LOCUS       DAYAOY010000001       114939 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYAOY010000001 DAYAOY010000000
VERSION     DAYAOY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 114939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.001"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(43..582)
                     /locus_tag="ACGI6B_000001"
     CDS             complement(43..582)
                     /locus_tag="ACGI6B_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749479.1"
                     /translation="MLNEDVVKKLKNVPNNTNTEIEKVNTNIETAKTELNTKIDQLIA
                     GGSNVASTQTITIDDWVDDTENGFKATVTHGLLTQRIVVNIIDATTKENVVTNFKIID
                     DNSIEIRSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFVNLEEK
                     VNGGLSNIATSVNELITYC"
     gene            complement(662..985)
                     /locus_tag="ACGI6B_000002"
     CDS             complement(662..985)
                     /locus_tag="ACGI6B_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HFG9749480.1"
                     /translation="MIKIDKEKIEKSRQRKELDDLDSSQKTQDNDITDLMLATAEICE
                     MVLSSQQTSTMSLKNIKLNEGGSSMAAIYVGLIERGLKTIDQVPVKYREEVRKMCEAL
                     EISLS"
     gene            complement(997..6276)
                     /locus_tag="ACGI6B_000003"
     CDS             complement(997..6276)
                     /locus_tag="ACGI6B_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749481.1"
                     /translation="MKKNKLLQRGSLFGNKEFKIEEFNKNFDTFNVKDIFTDMDSGSV
                     FIIKDDLKLYAAGYNVFGNLGLGNKVSPIRYFSKIDIDNVKYVSSSGTHSLLLKNNGD
                     VYSTGLNTDGELGLGDNIDRNTFTKINISNVKQIACGNGYSMLVTNDNELYVCGNNKF
                     GGIGLGNTSTVNTFTKIDNLSVKEIFAGVSCSYILTLNNELFSTGLNNYGQLGLNDTT
                     NRNIFTKVNLDNVIKISTAQGAVNFLLENGDVYGCGDNSSGQVGVGGGATVTVLTKLS
                     ISNVKNIESGRYHKFYIANNNDIYVTGANPDGRLGDKINCDRSTYTPRKSSISIKNIS
                     KIKCAHAISFILTSNNELYMTGDNNYSFSYLKRYDKFIYGFEKFEDFDIEECNHIESI
                     DNRLLINKNDERVLDIGLENNFVNISLVKANDTSTFIVVNGNELYAAGYNAYGGLGLG
                     HNRHCYEFTRVPLELEEGVTIKDIYLNLGSYFTFILLSDNTLYSTGYNVFGVLGLGDN
                     INRNTFTKVNISSVDKISVGDNHALLKTTTNELFSTGLNNYGQLGLNDNTNRNIFTKV
                     STVTYVNEIIAYSNSSYCIDNSRISYAAGYNVFGELSLNDTSSRSVFVKMKYLRYKTD
                     TTVSDVSNIAEIIPFNNGCLIRTTSNNYYVSGRHNYYSTSIKNSVTDDYYSRFGEFVG
                     SKNSNANEVAVLIDEPKLSKVLVSNYNLCLLLDASDGGYKEIKFTGRPSTFGASSQDK
                     NLGLRSLISSSMPYYNHYLGTDNLLYTTSKTRMESKFEIRGRNAGGQRGDSSYYSNSQ
                     KYTLSNAFFAYNSQVIYASRLRIVIKDYFGNIYCSGVNSHGCFGIKKPYDVRHDGFVN
                     ISKQFSKKYIISNIKEIKSDRQALYILLNNSEGYVCGNNAGNKLGTIKARSEFIKVPI
                     DNIKEFIPSNDCLFILTNNNQVYSIGTNDLGQLGLGDNIDRGTFTKVPIDNVKKVITC
                     YKSSYILTYDNELFSTGSNGYGQLGLGDNINRNTFTKVNISGTIKDIFCGRYVFIILL
                     ENDNIISLMGCGNRNYISSNTNSNSLTKINIEGLTDPSTISNLMLEEGKTIITMKNRI
                     FVTGYNSLGGLGVGTSYNNSSGYIEGYKDLAFNSNIKEVALGEYTIFVLLENGDLYSG
                     GNADDGIGFNESGTVVLKKLTSNIKHIYANGKNAYYIDNDNALYVFGSRQLGQINNYS
                     DVVQKTPVRNCLNVKQVYTQHNGLIVQYTNNKLEGKGNAVVGQVGIFNGHEENQIANT
                     SGDNPSDLSFSNEMSIIRNNYGLLVNKKIFLHNDDSINYRIPFGTNAGNYSTSLPFDN
                     IKKMSLSSTHSILILENGDVYGCGSNQFGELAINKSELTTTSEFIKINLTNIIDIACG
                     DNFTYFLKNDGSLFSIGKNSEYQLGIGHNDEVNELQEVLTISNIREIYAYSNYILAIT
                     TEGQLYVQGYNINGILGLNENTKNTIIKLFTKVLENVKYIKSYDDKHIFVIKNDKTLY
                     VTGINKETYKIKDVEIGSMLYTFSQIYIPRDVNDIVDALIKDETLYIISKVDSEKTCL
                     EIKNKSYSSIEIDLQNPNNELTKIEMFINNISSSIIEDLSTGTVTFEINPENLVLGEN
                     KIVFKAHSDIGNNLYINVYIYKKETGATIVKDSTVLIDGNTYNVSSIVDNSQDVVLTL
                     NKGLLEDLNSNNPIYHLVNKLKVQLKINESDTFKDMVKVETRKTENGYKEIYELKDMN
                     IQSAQPKVIVEEGNTNTTIKKPSMLFNLDVETL"
     gene            complement(6287..8365)
                     /locus_tag="ACGI6B_000004"
     CDS             complement(6287..8365)
                     /locus_tag="ACGI6B_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFG9749482.1"
                     /translation="MKNYEIKNHTIEVDFSNYRINKRLLDYFIYNENDVKTAYIEAIL
                     KNKDEIIDLSEYDRVLVSITKSDGQKVNGECEVVDAENGIVEIELSRQALASVGINTF
                     QLSLVKDGTLLNTTNLYYRVEEGMINDDDITSTDEYGVLLVIIAQAEEIIKNNKELTK
                     RVEQLEITILGNEEVRDKAEQIRIYNEDIRNIQEEEREFNELTRQNQEANREESIQNM
                     QIQVDDKLTDCQLQLDEMIDAKSKEIDNIVDDKMLDVQVQTDKKFQDLDTRANNTFDL
                     YDKTFEDKLTDNQEQIDYKLDEVNQAISNVEDCIDESTTKLDTKIKEVDDKIVEVNTA
                     KTDMTTTVSNKITEFENRFEELESLDARGELIQARESVDGTVKDTLKDRLTYDFEKVN
                     EKIAEMTSAATNVAFSKSYVESDWVADGEYFKLIVNHNLVTENIFVAILDEATKKSMT
                     NSYTTVDSNTIEIFNESNIDVKVTVVNGNTNKEVIQATINDNITTLDSTYSSIKIDAK
                     FDESLTKINENKSNIATNLEKINLIQSKVGSSELSTVSKNISDAVNELDASVKVLESG
                     GNTNEQLKILKENYNKMSVEILKILFELELDKEATADEAGYWYDTLYDSKNISNLDGL
                     KLNKFKQQLELLSNSGTVLFKNVTVPFLCNKIRYIHDLGSNYSETKTEESYQKGDTEI
                     KINKYSYEVR"
     gene            complement(8377..8532)
                     /locus_tag="ACGI6B_000005"
     CDS             complement(8377..8532)
                     /locus_tag="ACGI6B_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749483.1"
                     /translation="MEVNLQKAYTVAFEEIKSLYNELILYKALNMQQQEEIENLKKEL
                     EEQNKEQ"
     gene            complement(8534..10456)
                     /locus_tag="ACGI6B_000006"
     CDS             complement(8534..10456)
                     /locus_tag="ACGI6B_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFG9749484.1"
                     /translation="MILDNSPADSWIRITGVIVTYNNTLYQVVDTETNKKYIYWDADN
                     PGTLKVSNVRLPEGNTQFLVVVNDNGKHTEVPINSSIFDISFDGNSRKNTEEQIWGLY
                     ETDEKHNEKFVVIEKDIDGIHQTVLEVQEDASHIKENMSLIDQRAENVNILVKEVTKN
                     FGGSQENITLRENINKAIIKLNADLGTFSSNMSNYFNDNEVTDEEKEKIDIELNLLDT
                     DKASLYTELQKLIDRTTGVDLVAINTSKTALDAASTNLNSIINSVISDSIITPSDRIL
                     AINANAQYNLKINELKNTVDKIYITGMGGSISEEFSQINATAKEIKLEVAKVDGATKT
                     NAAEIKLTKDDITMMVTRNNSGSIVGIKPDKIEFGFNDISNYVEISRSGLTVNQGAIA
                     CDILTTPSGHEPIIRLFGSSRSGFAIDARQSDGSSQASAIRLKYDSNNYFWVGYDTAE
                     IYVDGEEHFIVERDDTFVRCGGATFTFTNGDSLGVGYSFYPERSVTDLGCERYKWRYL
                     YARSTLSESDKKFKENIVYIKDIKNRTRSSITPTPFLDFIRDDFKPATFDYIAEKDRT
                     IADSQIGFIANDFKDSYVGKTFLYDYGEENGLMFSPSGYTTVVATALQEEIQKREELE
                     MIINELNEKINNLGGY"
     gene            complement(10458..12173)
                     /locus_tag="ACGI6B_000007"
     CDS             complement(10458..12173)
                     /locus_tag="ACGI6B_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749485.1"
                     /translation="MHFNVNLKQIKSDYTLTIHKMNKSFLGQIPINFLNSIKRELGGV
                     DEIQLTIPKYITDRFLFNKIINPIFEEVKEERLICLNNKEYFVVKNVVTTDDKLKVVT
                     AKSKEVKLGKIDVNIEDYGLQMFTKDEEASIISLNDYLKQETGWKLGYVDDSIAYETD
                     SEGNKREKVRWQESINSNWLDYFNNELKEQFECIADFDTYNNLVNLYHIDSFGDNIQL
                     YLSHDNYIKSLERTTNSDDIVTRLKLEGSEDMDIIGATVTGYDYIENYSYFLDNKEMS
                     EELSRAIKKYQEMNEIREPIWRELIDTKLKKQRERDSKSNEWLHVIETIKAKKDIKKT
                     YDNPEHKDEVNSAKLAVEISELEDKKVILDVQIKHLEEEIAKLDESIKDINILCKRET
                     STDEDGYLIFNEALLDELNEFLYYDTYTNDAFLKVEDLIAEGKRQLSLKCIPTKEWTL
                     DVINFLDRIIDINFRQHWKGDLSLGDIIVLHSKESKEEELVYFTSFTQNFKNGKLDTL
                     ELTLSNKKVKEDDKRTIADYLTKAEHATRTLNSKRHLFIQQQKKRINLPDEYIPKKNI
                     QKELM"
     gene            complement(12526..12681)
                     /locus_tag="ACGI6B_000008"
     CDS             complement(12526..12681)
                     /locus_tag="ACGI6B_000008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749486.1"
                     /translation="MINNIEDCKLLIEAEIRMKDIDEKELISQRDILKDLNINEKDLE
                     DIEVNFE"
     gene            12965..13099
                     /locus_tag="ACGI6B_000009"
     CDS             12965..13099
                     /locus_tag="ACGI6B_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749487.1"
                     /translation="MIIMITRIINHLTKLIDAILNLKASIRKLKNHQTGGSSDSESNT
                     "
     gene            complement(13831..19905)
                     /locus_tag="ACGI6B_000010"
     CDS             complement(13831..19905)
                     /locus_tag="ACGI6B_000010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9749488.1"
                     /translation="MEDKRKRGGSVLHEYGVSLGDTVGMITATNEAIQNPEKVGNGLK
                     SVAINLAGMKVSADDGTLSLNKTAKGLKEIAGITIYKNKDTGELKNMVQLLDELKGKW
                     GELREDEQKGLAEAIAGKQQAAIFQSLMGNYETFKEIRSEFSQGLQFGSAEAENKQYL
                     DSLAGRLNELKTIWADIFQTVFNSNTTYGFLDGLISVSNGINSVIKTLERLKLTTPAL
                     IGSFMTLKTLFSGMGMTNGTRNVARLINPLNMIGTPIKATTTILQSFRHAVMAANVAG
                     ANGANVITRAGTGLGALRQSLSRTSIGIGTLNIATSLLNGAMIGLATFAITKGIQWID
                     DTIHKYEKLAEKQKEVIGTTTSEISSLQQKKKSLEGISEEYNNLASKTEKTADETERF
                     SELKQQIADIAPELVLGVDENNDPILNMSGNLDEVINKLRKAIEEKNKLLKSQEDELA
                     ITTSKTAPDTKKDLDKEKKKLDKVLEDEHNLFKNALSKSNFKEQTEALKKAYNQQETD
                     FAEHHQKLLTEYEKYNEEEEVMQKRAINRLTNSDISKTYAKQSEQKKSKMNELVANMN
                     WHDYSVGQQNSIISAFDKISESIPVEKLDQWNKKITDANINYQASGDMNQYTKELQGL
                     ANELQKLSGGSISADTWLIGLQQGFSGLDLASMKLHTFLKSYGSSLAELENKDSFAIK
                     LKNQFDALNDFSQDLEVNGKVNIKLLEDIQSGEAGQDLPQQLKDIINGFTFGEDSQIE
                     AQAQLALQVTTTLQNEGQLSNDVVKQLNDLFKGKLQLTDDILKNGLKISDKLPALPGN
                     MVQALNEQFANAFTGDNAPEITPSVNGEKAKKELKELEKFKKDTFKSDKIVTEINNKG
                     LDFAKKDLSVLKEQLDRFKNEDTKLSFTTDCADAFKDVNSLEEARKKISAPMSLKYDI
                     DVDKKGLTEAKKVYDILPDNLKKKIETEVIGIDKLDTLQNLIDKAPNKKETIFEIINK
                     NPDKLNEIDEILNNVPNDKKEAVIDFIVNNLDQLEKAKNVQEFINNLPPEIKKQLGIE
                     VEGGENAEKTVNTVQNADGTKADLLVNAEVNGESQVVECIETLDKNGNKTYTPNLNPQ
                     VNNSEKVVQCMRILDEYGNESYIPIVSPQVTNEEGLNSTKQKMDELDNKTVTPTVQIK
                     TEGETEVEQATNFIENTNPTKVIELSINNSGKDEIDNTKQKLDELNNEKATPTIEATG
                     AEQASEQLNQLSQKAQEIGQGNYHINVSTSTEQGAKNISGLIARVKQFLALKVSTLVF
                     RTETAQAAKNVSGLLAKVQSYVSRYGGKTIKTTFSALTATAAKNISGLIRKIESFKSN
                     YAGKTFTTTFVTNKVTNSSGGGGGGNSSDKTNKTERTSVTNGTPIPANLSQPRTSEPA
                     PISDETPVTKPSLFSRAVSRATSAFKVIKPKVTINNDTIDSSVKYSIELFKELENAIS
                     AVTNEIALLDKKMEHATGDEKIKYLQEQNKLFAQQKDLIKQQQDGYKAESNRLKNRLK
                     EKKMGFKFDDTGNLTNYEEKLISIEKELDKLNSKSGDKENKKDESRKKELEETKKVLE
                     EYIKVTFTELPKCEQEWEEYNNKIKEHNEELRKAQEEMWKLNKNSTWTSMYKDVEQVN
                     NEIAMIDVLLKNATGDEQIELLEKKKELMAKKAKELSETTAYLQETQKELQQKLQGYG
                     FTFRDNGDITNFIQHKNQLKKNMTDEEFKRMEDAAEGYLDLLINKIPDANKEIAESKK
                     EIEALNKEIQDAYKDQLKEAQSLQEKIRDMYKKELEERLKEIDKETKAKIDSLKKQQD
                     AYNDSRKEAKYKDDYEEQQDVISDLEKQIAIAERDSSLSGQKKLKDLQKQLKEEQKKL
                     QDLVQDHVDDQVNDMYDKESDRLQEEADKLKEELEKKYSDENLVDLINEAISSGKFVG
                     LDGEVEKLQDAIIEYINKYEDGMLAMGSVTKQEWLDTLKEGKETLEDINDILDELDLS
                     KFAMPNYNPPSNSRSRSAFPTSSVNFNSPFFVVQGNVTKDTMKDLEKFGRDLENRVYR
                     KIVENIKS"
     gene            complement(20509..21987)
                     /locus_tag="ACGI6B_000011"
     CDS             complement(20509..21987)
                     /locus_tag="ACGI6B_000011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749489.1"
                     /translation="MVEEFKIKTSIELDDKDAREKIEKLKNSGQDKPIKLNLKIGELD
                     KLSQQAEQLKGIFNKAFKIDNTALSNLKQIESTMKEINKLSRRSQESLLGGGSGNTGN
                     INNEQQDLIRQYKEISNVVSRLQNQIRTGLGDSSIERTTEQIERLKRQLDSVYAQMNS
                     SSQARMDMFDASRATRDLADMNRNLSRIEDMATQASARLNGLGDNIDTDKVNRLRNEL
                     QELKQTASRNIDLNLDVGNIVNRIQEINQEVRNLERVDSLRSNFSRIEDSIRQAFGNE
                     RVEAFRRSLEQLQGSAGNLNGDFNNIFDRLTREMRDARQEAERLNNTVSRGGGFFNDF
                     YSSVASYSIGDMLGDALTDGIRSMKDVILELDSSMTDLKKVAEPTDIDSTSELDRIRE
                     KAIQTAKDVGMSASQVQESIASSLQAGIGGMQESIAAARQSMILANVGDMDVEGASSA
                     VNTIINGFRIEPLKEMQVQVGNTTKKTTELTAAMDMLNCLAC"
     gene            complement(22067..22516)
                     /locus_tag="ACGI6B_000012"
     CDS             complement(22067..22516)
                     /locus_tag="ACGI6B_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749490.1"
                     /translation="MFKKILLILMISLLGFGVIGCSDSKEDTNEVVKKETEEKLDEES
                     TSEDIYNKKLQFLNDTKNQSEIIGDEIRLLTEKYNNYEIVKSDWKEDLISKKKIITDL
                     KSVLEEEKSNILDGVSDFQKTILQNKYNEIIEYYDYMLKGISQNLNI"
     gene            complement(22605..25832)
                     /locus_tag="ACGI6B_000013"
     CDS             complement(22605..25832)
                     /locus_tag="ACGI6B_000013"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFG9749491.1"
                     /translation="MEKYLFNQKLLKEKYNMEIDLDLYDYKQRRLILEKWRDIIDNKS
                     IDKLNEIQLKEIFVSDILNKVLRYKSLTDNIMDYNIKPEEKTKIDGTRADIVLGYFSN
                     EESDYRVAIELKKPTTNLDAKQLRKNNHNTPVEQGFSYLPKYGRNCTWLIVSNFKEIR
                     LYNANDATEYEFFSIKDIYEKDDVFKKFLYLLSSKSLMTKHGKSRVEMLWEENIKQEE
                     AIEKGFYDLYKNTRLNLFNDILLNNKDILDVKTCLTKTQKILDRVIFVCFAEHKGLIP
                     ENIFETCIQVGKKSVSRFGVWEQVKGLFESIDLGNQEKGINQFNGGLFAEDYILNNLK
                     IPNKCFENLYKLSLYDFNSDLNENILGHIFEKSISDLEELKLEINQVNIKKKDSKRNK
                     DGIFYTPKYITKHIVNNTIRRYLDEKKKELGEDKLPELKEEDYETVVVGRNIQHKRVQ
                     VVEYKTDKIQKYINFWKNYRDVVKNIKILDPACGSGAFLNEAFSMLKKEAESVNEILR
                     DLTDGEISFFDLDANILKNNLYGVDLNEESVEITKLSLWLKTANKYSLLTSLDNNIKC
                     GNSIIDNKEVDCAKAFDWNVEFEDVMMSGGFDIIIGNPPYVSTKMIPEKHREYYWKKY
                     KDLLISEMDLYELFLYEFCENKLKQDGFLGFITPNTYFTNKSFENLRKYLLKNVCVDT
                     ILDFPYRFFPFEDVNKETAIIIINKKLPNIKNNINLISINKENMKKIKIFNSNTYNSE
                     NKITIKSIINDLDNKIVIQSNPIILKMLKCKHKLGDYLNLHKGWMSVPNKTICDGIIY
                     DKKILSSNDIKDNNQLNGILNKCLEGKDIHRYYIDKVDKFVNIENMDDKTMRWHKSPK
                     IITQRIVGQNQNKIIATVDIEGTIIFPSGNIINLIKEDNLEDIYFYIGVINSSLINYF
                     YNKFYGESNTNLTADAVKNIPIPNLKNVDKSIVINNSKILINKYGKLYLYTNKFLKNV
                     FKILSIDNNPSNKLYNFYLLDFDEFYNELSKKKKLYFKNIDELEEYFNKYKDAINRII
                     YDINVCENTINTFVYDLYGITELESKSIEDICKTTKDTVVTSLAY"
     gene            complement(27327..27764)
                     /locus_tag="ACGI6B_000014"
     CDS             complement(27327..27764)
                     /locus_tag="ACGI6B_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749492.1"
                     /translation="MVFKSLDELVAYTKNKIAASMPEVGKEMKEIVKEEVNKQVYSDY
                     APSLYDRTYELLNAIDVSEITSDSVATEIKDSGSWFDIYSKAHAFPMERFENGGVWGH
                     GSTKYNPVFRPRTNIMEESNSKAQERVPKCFKSKMNELGVPVK"
     gene            complement(27765..28214)
                     /locus_tag="ACGI6B_000015"
     CDS             complement(27765..28214)
                     /locus_tag="ACGI6B_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749493.1"
                     /translation="MNKIQVDKLIQDEVRAIIPIIDENGKEEYIEVRNPDKETKEEIL
                     NKIWVGMENPDLALSQEDILKMLIDKLTNIELNIEIEDLVDGNISSELENVMYYIGQI
                     ENELTASLLMNTEIKLGQMKNEILQDRVLKETEEIEKMNNIKEKVVN"
     gene            complement(28228..29031)
                     /locus_tag="ACGI6B_000016"
     CDS             complement(28228..29031)
                     /locus_tag="ACGI6B_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFG9749494.1"
                     /translation="MKEGDKVVFLDDKFLFDNISSESMNIKLVTLGDDEILNEYGLPY
                     EEAIKSDSTFNKNPCYSEDETTIEPITLQFCLCSEHGEAYEWDDYTLENICNWFWQRE
                     FKPFISYDNIEEIYYLKARKIIKRYTKDKKGVLEIEFQPYTNYAYRSFQKVITVKDTR
                     EIKLNNVSNVDEEYAPVIDIECLKEGDITIRNSTISDNEEDNLVISGLELNEKITIDN
                     LYYTVLNSSGENRFNIVNRKWIRLRRGVNILKFTGNCKVSIKCKYPIIK"
     gene            complement(29110..29925)
                     /locus_tag="ACGI6B_000017"
     CDS             complement(29110..29925)
                     /locus_tag="ACGI6B_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749495.1"
                     /translation="MRFAIKDASNIIVKNKTTGEPLFYTADLNAFNFKLDSESVYAKA
                     KGANTIAFDGAITASLALEQEVIQMPQLAMLLASDMVEESAKVGKRKLLTSDGTKKVT
                     LENVKPVTNSISAYSVEKDGISLVKKLQFTSTVTGSNTEITISTADFNAGDKVAVFYL
                     EELPKAKVIKIKEESTAPNYVIEADVMAKTADGEYMVLYMTIPNAKAQRSIELNLTAE
                     NPSGFNMTLDVLPDENKEYVVFAFVGDEDVSPVRMASMLGAELIDEKDVKSKK"
     gene            complement(29968..30744)
                     /locus_tag="ACGI6B_000018"
     CDS             complement(29968..30744)
                     /locus_tag="ACGI6B_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749496.1"
                     /translation="MLENYYITGLPIKLGETLGTVYQPTIEELIKFDMANLEIVNPFL
                     VLEKSYSQLCNEKIFELKCKYDAIPILDSSEEIELLSDKIKKSLSILYKTDIKNIEYM
                     NNIKIGILIKFDDKKKNAFISREDFGLVSDLILEMFCIDKKNLFKDDEDKWIENTGSE
                     REKQLIAHFKEKERKKREKEAYHLCDYINVVQNIDGYVPIDVILKMTYWQLINAYKTK
                     IQFKNYDESLGFAMSFKYQVEADKIKHWSKEIKIQTSTVK"
     gene            complement(30744..31220)
                     /locus_tag="ACGI6B_000019"
     CDS             complement(30744..31220)
                     /locus_tag="ACGI6B_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749497.1"
                     /translation="MISNIGATLMSNQDFAKFMIYNDESQKDILSMPDIKNPVKELRN
                     KKVFLNRRVEKVLKEADISVFIIMSEYRPCFEGSRTIKKTKIEIGVVCHDECQSTANG
                     LRDVALVCCIVDIVTQNEEIAGIGKIKLENVYQMYNLNTDYNGFVITVSAESFGDM"
     gene            complement(31253..32242)
                     /locus_tag="ACGI6B_000020"
     CDS             complement(31253..32242)
                     /locus_tag="ACGI6B_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749498.1"
                     /translation="MSYLSDTKKRIGLGCSTPKEKRILQLRLSFKKYLKETPTCIEVP
                     ITDIDEICITEDTKRAIVAINDITNNDKRALDEKNLLVESDLDVDVGCYLFYDNCYWL
                     TIFKEHKEMDTYKHFIIKRCNQFFNYKYKGQMYKIPIAVENLTLYSDGMADNKYTSIS
                     DTKRQLYFGSNPVTKTIDIDTRIMLTGKTVFRVTSINDFEYNGRETGADGLIKAICLQ
                     DALISKDDTINNIAWNDLSENESVIIPFSKIMGDGFINLGEENEYKIDHSQGVEWLLD
                     KQYGYCNIINQDEKKCVIQANTLAKYSGFEALLLAKDKDTNKTIDTKKITLRG"
     gene            complement(32244..32873)
                     /locus_tag="ACGI6B_000021"
     CDS             complement(32244..32873)
                     /locus_tag="ACGI6B_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749499.1"
                     /translation="MGTPLVKIYKKFLDGINDEEMLLLSNEIIEKMMYSYLEDAIVDF
                     NQCKKDLTIKYVDEKGGEIIPAAQLSYTSNYSNKNAEITLMGKDTKEEYELDKDYTIS
                     FEDEKFIISFVVETTEEIIFKYKYLGEIVSDLDLDEIKILSYGMQIHWLQPKINREEN
                     LKQMLTDSDYNAKSGANMLAKLQAREEQLRTRFNKYQQRYMLKNFEGWN"
     gene            complement(32888..33520)
                     /locus_tag="ACGI6B_000022"
     CDS             complement(32888..33520)
                     /locus_tag="ACGI6B_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749500.1"
                     /translation="MARAKKENISKTEVKVNTLEKDIKSTLNKKEARLNRLELQRKLK
                     QKKQEIDIEIKNISTWDVDYIDPREKQQIFSLSKVGSKDSTEFIDLDTLYRIVRRSPG
                     FFEEHRLIISDVDSLDVECTPMDIIDFLGLNSLYEYIRNPNEDYLEYFLSDKVDINSF
                     EKILNKNNVELTRRLAERAIDLHKKKKFDSGLKAKLLAKRIGMEDLYLFS"
     gene            complement(33574..34584)
                     /locus_tag="ACGI6B_000023"
     CDS             complement(33574..34584)
                     /locus_tag="ACGI6B_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749501.1"
                     /translation="MATAKTLKRIAHELYTDTFREYTDRDGNTITYRDAESAIKNKLK
                     EMMGGEKYNYYKFQENKWGFYNLISELISDDINRLNEEVFSPFCDFENFDLGDKKEFT
                     VENTNLFKVANIADGVNSTRRQRLLNKKVPTTAFKLSVAIYEEAERFITGRINWVVFV
                     NRVSDSYHYDIARRIAKTFEGAYSTINAKFQATGNSDKVLLELIAKVEGATGKKPIIY
                     GTPLALSNLEGVQTDLEKEEKRKYGFIQTFRGGYKVLSLPNAYDENAAEGKEWALDNK
                     AIYVIPDGEKIIKLGTEGDVLVIENTDEKERDDQQIEYFMAQKIHLGVVTAAKFGVYK
                     IQ"
     gene            complement(34672..35169)
                     /locus_tag="ACGI6B_000024"
     CDS             complement(34672..35169)
                     /locus_tag="ACGI6B_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749502.1"
                     /translation="MKKDKAIIQTDKVKNPDVLTGKYVVPSEDTNPINLENGAVINIG
                     ELEDTEYGRDTHKIYKVTNDTLDWGIVDDPATMYDERLDERDYEVSPGQICRCRRLKK
                     GDEVTISLLHIADKSIAVKDKLQLKSDSFQFEKLPTADAKTPVAEVLELWNYEGQDSV
                     TIKVL"
     gene            complement(35184..36617)
                     /locus_tag="ACGI6B_000025"
     CDS             complement(35184..36617)
                     /locus_tag="ACGI6B_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749503.1"
                     /translation="MKKIKILKLPCNLKHYSDKKKLEELNSNLLPVYIYVMHEGTNPN
                     GTKFYEEAIDKAEPTLKNVPILGYVKINEDGKYDFDGHNVLTQVVQTDEGFILEEYYE
                     ERIIGVIPETNKYEKVEIDGQKYVKCKGYIYKSYSNHAYDIIMDSDEIEISMEIDIND
                     YQLDDSDGFYNIKDYVYHGITCLGSDVKGAMGSNCCLTKFSRKINYKEEISKICSEIY
                     ALEHGEGEKNLPNKNQKLNKNSEGYALAVSNLSTEIRNKLKEYKVETENWYGEKVEVQ
                     AFYYNDLIPEENIAIVEDEINWGYYYGIPYIVNEDAVILDYENKKSYIQTWREKQEGE
                     IVQVFSRQEKLKKEIIEKFTEKQKEISNLKASLEPLQAFKEEKEFELFKSKVDDVAQK
                     FELAEDEIKDIKIKAYNKEITLDEYKKELGYIFALKTLSNKQSEKENFSINDDKNNTI
                     KIPISNNNDSFSEPEEISFIKKYSDKE"
     gene            complement(36624..36815)
                     /locus_tag="ACGI6B_000026"
     CDS             complement(36624..36815)
                     /locus_tag="ACGI6B_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749504.1"
                     /translation="MSKFIYAFSEDDKKLLMEKGYRFICENKLNNKTLYVFENKSKLI
                     NNFSNEEMKRFIFTSKIRF"
     gene            complement(36818..38296)
                     /locus_tag="ACGI6B_000027"
     CDS             complement(36818..38296)
                     /locus_tag="ACGI6B_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749505.1"
                     /translation="MGKKNNNKSKSKDNNSTQNQINMLNAQIGKYASVKESTTLATEM
                     YKLRSISRDKLRKALSNPYQISNTDILQDASMILKATSGTYRRVLNLISNMNTFDHIL
                     YPKDISRLKTKEKITKAYMNSASQLEKYNIKFTSAWITEKVLELGEVYLYKIEDNSGM
                     VLQQIPAKFCMITMIENNVMRYAINIKKLTDKNIISFPEEIKNIYKKYKSNLLTREEL
                     IDNTYFQLSDKAVAFNYDLDSVKGVPFFCFIFDDLMELEDMKDLKSTNAVIESIKLIH
                     QKIPFGKNDEPLVDLNLIPIYHNSTKANLPKGTAITTNPLELETHTLSDGKSKINDYV
                     KEAKEFIFDNAGINTALLNSDKINNESILNGIIADSLIPMRIQQMIENWVNFELNKDK
                     KANLFNMKFIGTTHFNKMNLSKQYREDMGYGGSKSLFIASTGFTPLQAINTLQAEKLM
                     GFDEFLIPQQTSHTQSSGRPDKSDIGTDNGNSTQAKGNGEND"
     gene            complement(38313..40061)
                     /locus_tag="ACGI6B_000028"
     CDS             complement(38313..40061)
                     /locus_tag="ACGI6B_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749506.1"
                     /translation="MRTKYEKRELSSNDSSRNLLESIGEYWGAFYLANPHRFCMDYFG
                     FNLHLFQQILIYMMMKSDQFVFIASRGLGKSWLLGVFCCVIAVLKPGTCVLIAAKRKK
                     QAKLLITSKILGDLYLKSDTLKREIKSFQVNAQEVSIDFWNGSRIEAVVSNDDARGYR
                     ANVLIVDEYRMVDEGTVNDVLVPFLTNPRQPGYLQNPKYRYMQEENKEIYLSSGWYSQ
                     HWSYKKFMETVKGMLSGEDMFACSIPFTCSLEHGLLTKKRILKEMKKESMSDASFMME
                     YCGVFYNESDDAFFKSSWVNPCRVLESMFYPPSDIEYLENKKKRDKKYHLNKIKGEIR
                     IIGADIALARGVKNDNSIYTLMRMLPNEGTYKRCVVHIEAYNGMEAEKQAIRLKQLFS
                     DFQADYMILDTQGIGTTVWSYIQKANYDSDRDEWYDAYTCFNEDNTVDKSLAKKSLPV
                     VYSMKAYADENHKMAMSLRDVLTNRTLELPISDIEAKEMILEKEMIKADEIDKKAELE
                     AKYIAPYLQTTALVNELINLEYSADGGKIKIKEKSGARKDRYSSLAYTNFLADYLEEK
                     EKRKNRNNQKTVMIYW"
     gene            complement(40051..40950)
                     /locus_tag="ACGI6B_000029"
     CDS             complement(40051..40950)
                     /locus_tag="ACGI6B_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749507.1"
                     /translation="MAISKTKCLNCGEEKYTDRYFWKSHSEIFTLNKRLPVCKECFRA
                     RFLLLNGCYNGELVKALKHICFNFDVYFDEKLAKELADKKNKDELIDEYMKIINRNSK
                     YKGKTSLDNLLKEIYTGNNNDKNIVINDEIKLKWGRGFDDYEYKILERKYKEYKEYYE
                     PESLTERKLFEEICIIELEKDKSREKGDMKAFNDLSKLVSSKMQDAEIKPSQKKKAGD
                     SQDDTFGMKMMIYEKNQPVKDRLKEYEDVDGFEAYVNKHMKKPLAVALGLATGKYSIN
                     DGDKDIKFKDDVRDILEGNKNED"
     gene            complement(41081..42280)
                     /locus_tag="ACGI6B_000030"
     CDS             complement(41081..42280)
                     /locus_tag="ACGI6B_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749508.1"
                     /translation="MEDILKKKEDESLVDYRIRIRLAKVNKEIDLDWGEIVELLGLEC
                     SPDHCRKVSYGLKEAFDYLNSKIQNNSTQEEIDKINEKILELKKIKVQLSDERSLVNK
                     KIREYSRIDNIIDLFNNKIDDISLHKPFLSDSSYKCYESSNQEAIMLISDIHYGLETI
                     NAFNRYNSKIFKIRIQYLKDKIIEYSKLHKVKRLHVMLLGDLISGYIHNSIRLENREN
                     IVEQIIEVSEILSEFIYELSKEIDKIIIYSVGGNHDRVLPKKDENLDKDNFTLLIDEY
                     IKLRIKNINNVIFQENIYDNDIIVAKICGNTCFGVHGDKDKMSTAIPKLTSLIKLIPD
                     YIFMAHLHNCKEDNYGESEICVNGSFSGTDSYAKNLRLSSHAMQKLMIFNESGRICTY
                     NINLSKL"
     gene            complement(42357..42545)
                     /locus_tag="ACGI6B_000031"
     CDS             complement(42357..42545)
                     /locus_tag="ACGI6B_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749509.1"
                     /translation="MKNNKGTVLEKCEIIKQENNTYKIIEHLKDGDHEVLLSDVLDKY
                     TGDTDLNITIRLSETIVQ"
     gene            complement(42915..43532)
                     /locus_tag="ACGI6B_000032"
     CDS             complement(42915..43532)
                     /locus_tag="ACGI6B_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749510.1"
                     /translation="MVKYNVHKALAEKKLLEDKIDRCINNFKIVGTKKGSDKNVYETK
                     TSVEDFNVEVSSKYQQIEDLIYNYNALDKAINISNAITNVQISNKNYTVLEAIKRKNS
                     IELDKSLLRQMVSNYDCMMSEVNRRNEEVQKNTDRMFEEKEKSKDGAELISFYKKQQE
                     WSLVDPLKVREKIEKLRDEIEEFEKEVDFALSTSNALTIIDVDLR"
     gene            complement(43588..43678)
                     /locus_tag="ACGI6B_000033"
     tRNA            complement(43588..43678)
                     /locus_tag="ACGI6B_000033"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43643..43645),aa:Ser,seq:gct)
     gene            complement(43687..43760)
                     /locus_tag="ACGI6B_000034"
     tRNA            complement(43687..43760)
                     /locus_tag="ACGI6B_000034"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43724..43726),aa:Ile,seq:tat)
     gene            complement(43779..44609)
                     /locus_tag="ACGI6B_000035"
     CDS             complement(43779..44609)
                     /locus_tag="ACGI6B_000035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749511.1"
                     /translation="MNGEKLIENKELRDRNIDRVDVLEHVKEILTLANTDFSTVELVS
                     EYYEVDRDTIKKLIQRNRIELELDGLKLYKYNDIAEVLENTKKGLDVTFKIPPRGLIL
                     IPKRAILRIGMLLRDSEVAKEVRTRLLDIVHDAEEQTEIVIEEIRNEQNIKEDMLEAI
                     ISGDHNKLSVLQTELIGLKNKRISHLEDVITNSVTITESKAIINKCVRTIAVKKFNCM
                     FGKAWDEFYRFINYKLGINVRNRKGKGLARFSDEEILKMEKIAKSWLEENDIDFKLVI
                     "
     gene            complement(44897..45175)
                     /locus_tag="ACGI6B_000036"
     CDS             complement(44897..45175)
                     /locus_tag="ACGI6B_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFG9749512.1"
                     /translation="MKKVDFLKEVAEELGVSQIRVREILDVIEKKRNEVLQDGEELTL
                     MGVKYLTKIQKGKEGEITLKDGTKKPWKTEEKRVPKLKIAKSLKDAII"
     gene            complement(45309..46751)
                     /locus_tag="ACGI6B_000037"
     CDS             complement(45309..46751)
                     /locus_tag="ACGI6B_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749513.1"
                     /translation="MIYNNNNFYEEIDGKSAQDYIKNLDYSIDNSKDRIKYIEERLGV
                     KHTWFEESNRNYIDTFYNTLTKRNENIITRVKYQEKKQFNDKFWENIFEQTSDSPLDK
                     DGVYYVTIDGEEVVMDYNRFITWCGYNQINPIEYINVKNPFSNSNGTWEYTHHNTSKI
                     KLILNKDDSIYSTSNIAKTLEIMGSYILSVDDKPKETKIRIYNSKELFNRACQEEALL
                     NKIAPANGGNINSNTSNKDGFLEDNSFAFFQLPKNYKKIKDIKVKPKDIKKYPIIKTY
                     SDTYEWYKAKYKELGHKQLSKEELKLKRMVRKNLNALKNDMTDVKNSIERPIIWKAPL
                     ADAGSPEWDYLDMFDKSHVKELLRIQKGNDLQDDLTCIVMDLNNIISKIEFTTVQKDI
                     LNLYRKDKSLEFISNLMNITPQAINNQINKIVNKIIDAYEKNYEENYYYISVCKGKYK
                     KCSRCGKIKLLSRFDKNGKKGYRSNCKNCN"
     gene            complement(46754..47002)
                     /locus_tag="ACGI6B_000038"
     CDS             complement(46754..47002)
                     /locus_tag="ACGI6B_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749514.1"
                     /translation="MKEKKIELIYLEDLDESEKTGVHKNRYQILNEEYEQMIDTYTEM
                     SKRSSYDIVNERHKEDFKDELWDYDDTDYDDGLQGKEV"
     gene            complement(47180..48043)
                     /locus_tag="ACGI6B_000039"
     CDS             complement(47180..48043)
                     /locus_tag="ACGI6B_000039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749515.1"
                     /translation="MKRGEVLEIGKRKKSFSPEQRAIINNKKELPYHNFKLGGFVFCT
                     YVKNELLFNEVNISKANISRLIYLATYLNYNTGEENLLVRYSQFKEMIPIDRKNMREI
                     LNLKDRAFRNFLADVKKCELLYESNNKFYINPKYFTKGIPNFENKEYTRIYINTSRFL
                     FEHCTPRQHKQLSYIYQLIPYIHFESNILCLNPHETDISKIKKISLYKICQLLNISTI
                     KQDMNKFKKELLRFYIKVEDDKYYFFKYVIVEGNNKNTDYFIINPCVIWKGNNLNLAK
                     KTIDLCFFNCI"
     gene            complement(48315..48587)
                     /locus_tag="ACGI6B_000040"
     CDS             complement(48315..48587)
                     /locus_tag="ACGI6B_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749516.1"
                     /translation="MKKSSSFYLEEDIFGEIEKYQNDKNISSRNTALERIILEWKNLQ
                     EENKLLKQCLGNGVYRTNKKTNVTEEKKDIKRENPIIKNIFNNMPD"
     gene            complement(48600..49514)
                     /locus_tag="ACGI6B_000041"
     CDS             complement(48600..49514)
                     /locus_tag="ACGI6B_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749517.1"
                     /translation="MEENYQVIGLDIGRGYVKGYSKYNGMVKECLFKSVFGDGRNIDF
                     EKYENPIYIDFESVSYFVGSLAEKESITPIRNSDDSKVSFTMRILVAAALNEIAVADE
                     VKLMMGVPYKSFRKTTLKEVVDTYKGKTFKVKDKIKGGHKEIKISDISIFREGDAALF
                     HTLEGKVNEDKAVGMVSIGFRSTEMSFFEKGFVFNDKLSDTLEAGNQDALTMVQKQLK
                     DRGIIRELNEIDSSNDYDELKKVAYIMASESTAQRISSKWKNIDEMDVYVSGGTALHM
                     TFDNRFKVSKDAQMATAKGLFEVGMEQF"
     gene            complement(49936..50124)
                     /locus_tag="ACGI6B_000042"
     CDS             complement(49936..50124)
                     /locus_tag="ACGI6B_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749518.1"
                     /translation="MESGLFVSRILCVVLSFPHFKKIADGFTDEIALYFPHKTNIYLK
                     IFLRFLLLNYILNKENIK"
     gene            50620..50778
                     /locus_tag="ACGI6B_000043"
     CDS             50620..50778
                     /locus_tag="ACGI6B_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749519.1"
                     /translation="MRITKGKIKELIELCHQLEKLFVQVFKSLEHILISLCSCIGWLL
                     ILIYIIKH"
     gene            51031..51222
                     /locus_tag="ACGI6B_000044"
     CDS             51031..51222
                     /locus_tag="ACGI6B_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749520.1"
                     /translation="MKLEKLIDIRKEKKYSQEDIARMLDISLRNYHRKEKGENQFTVS
                     ELEKICSYLDIESKELLDI"
     gene            complement(51436..51747)
                     /locus_tag="ACGI6B_000045"
     CDS             complement(51436..51747)
                     /locus_tag="ACGI6B_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749521.1"
                     /translation="MNRLKEVRKERGYNIQEIADKLNVAYNSVLRWENGNRKIDIDKL
                     VELSNIYNVSIDYILCKTDLKENIQLKEEEVNLLDNYRELDTKSKTIVQEQINTLKKL
                     L"
     gene            52476..52799
                     /locus_tag="ACGI6B_000046"
     CDS             52476..52799
                     /locus_tag="ACGI6B_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749522.1"
                     /translation="MDEKILELLQKMDTSINDIQGEIKWINTRLDSMDGRFDGIENQL
                     SDLKEGQEEIKKKLDLTYNQVARNMEGITEVGEKIDTLKNDMNFVEMATSKNWNEIAK
                     LKSIK"
     gene            52811..53044
                     /locus_tag="ACGI6B_000047"
     CDS             52811..53044
                     /locus_tag="ACGI6B_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749523.1"
                     /translation="MDEKTLELLREIQESIKILNDKLDEFDYTQDKIKSNVEGLLECF
                     SRIDFRIEDLENGQTSLYTKLDIVQNETAKSIK"
     gene            53139..53327
                     /locus_tag="ACGI6B_000048"
     CDS             53139..53327
                     /locus_tag="ACGI6B_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749524.1"
                     /translation="MYEQRKAKVIFNKGAGNSRGESYTNRVTIPTTWIKHMDITKLDR
                     EVLLTFDGNKIVIEKIDE"
     gene            53448..54161
                     /locus_tag="ACGI6B_000049"
     CDS             53448..54161
                     /locus_tag="ACGI6B_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFG9749525.1"
                     /translation="MLNIYITNLGKYNEGELIGEWAEFPVNEEELEEILDRIGINEEY
                     EEYFITDFETDIEGLEISEHSNIEQLNELAKELEELEEYEIEQLKALLGCGYIDFYDM
                     LEGDICRLSGNYTFIQLDDRSPLDDENLGRSYIEEICCGDLSCIENIAYYFDIEAFSR
                     DLRFDRDMIIENLYEEDIEYYENMTDIEFAENYIERLGDIEELGQETLERYFNYELYG
                     TDLRYENICIASNYLAIVS"
     gene            54229..54369
                     /locus_tag="ACGI6B_000050"
     CDS             54229..54369
                     /locus_tag="ACGI6B_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749526.1"
                     /translation="MKKLIIKIKSLFKKDLDSSYWSTTESIILKCGNSNQLELLEQAN
                     KL"
     gene            54649..55344
                     /locus_tag="ACGI6B_000051"
     CDS             54649..55344
                     /locus_tag="ACGI6B_000051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749527.1"
                     /translation="MNKIIVFYDIEGHPGFQVLVRNVKKYTRQNIIFELVEDYIKNNI
                     GEIDLKDEKIYSSVSWKVWTISDVAIYNTLFDLRLDYNKLIESEEEDTDNKECVKELF
                     CMGKGCKWMETVLLLRQNKIDSSQYNILTINNYTYVKTWAEQCKKILLANNYSKVFKF
                     NEIMKDCDNNLIGDICFKYNNKEYKHKFTFNIKKDKLVNIDCEYKIPFLRYVFNDIEK
                     FLTRYSENISNLK"
     gene            55484..55669
                     /locus_tag="ACGI6B_000052"
     CDS             55484..55669
                     /locus_tag="ACGI6B_000052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749528.1"
                     /translation="MQELCSMGLTYPFDFGRGKGKYQTNIDEETIYKRAKENTKIQEY
                     SKKYLNYIAKIKKSISK"
     gene            55703..55999
                     /locus_tag="ACGI6B_000053"
     CDS             55703..55999
                     /locus_tag="ACGI6B_000053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749529.1"
                     /translation="MKKIINGYSIYKDGTWCEVIKEGKFIFAGSVNEKLSYEDIYIAL
                     TAKLEFRLHGKLLLSYSLYGTFQGEKQSTLELLAYENNCNINDIEVKQVVENIQ"
     gene            56136..56681
                     /locus_tag="ACGI6B_000054"
     CDS             56136..56681
                     /locus_tag="ACGI6B_000054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749530.1"
                     /translation="MKKIANLNKEELKIIWEKNSQLREDVRKACEDNDMYWIDEILCH
                     LNKSLDDWSIGFYNDNYIKIEDNHKFLLELKSVCEKFSFLSEEEQRPLEYAISLIDKL
                     HCMDFENKRFNMLENKINNMVKKLEEKVIEEFNKMTEPLDKEYLLENFIEFYVEAYLN
                     DNEFYIDNEYVLYEKIIKSYA"
     gene            56707..56970
                     /locus_tag="ACGI6B_000055"
     CDS             56707..56970
                     /locus_tag="ACGI6B_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749531.1"
                     /translation="MLANQIKGIKTSLETSSLRELKGYIKDLRKALSGYKYKNKIDEI
                     TEYQIDKLCYKIDNTDIFDLKYKEIEKYIDSLILILDFIFMEM"
     gene            57008..57592
                     /locus_tag="ACGI6B_000056"
     CDS             57008..57592
                     /locus_tag="ACGI6B_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749532.1"
                     /translation="MNEYILISYTKGEGTCDHCGRTIKNIATIKNNVTGEVFHVGLTC
                     VEKIMKLNVTFYKALSREIKKHYKCMEYYSKGLDIETNLNKIVKNNTKYKEGSYAYKS
                     NEQMLEDAIAEVAWSLARMIDSCMRMNKLSKSGLIDIDILNSLFIKYKEYMSKFDENY
                     NKNKYKYKSCYTMKPLKVILDENEDLKELYKSMQ"
     gene            57896..58417
                     /locus_tag="ACGI6B_000057"
     CDS             57896..58417
                     /locus_tag="ACGI6B_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749533.1"
                     /translation="MKFKNIVMGMLLGLIIVGVSGVTKVKAAELDNFYIESTDRVIEV
                     LEDESIVLYDTKEQVYNFYPICMGDWNYSFKNKKDLDSAVATYKELSSNINHLSNIYV
                     VNKLNDNGNIKVFLNDGSSIVYIKQDNKYYFYPSCMGDWYLTLDSKIYLTNCINTYIE
                     YYYSLSTGGGTNE"
     gene            58410..58640
                     /locus_tag="ACGI6B_000058"
     CDS             58410..58640
                     /locus_tag="ACGI6B_000058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749534.1"
                     /translation="MNNNDKLSILNQLEYIENDIDARNEMIEKFKDLLVKYPKSRDIR
                     RYIKQQTQFLNGIRYIYDNLDILKNMINNDKK"
     gene            58811..59620
                     /locus_tag="ACGI6B_000059"
     CDS             58811..59620
                     /locus_tag="ACGI6B_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFG9749535.1"
                     /translation="MNKLKVKGKQKFMNLEIPVIEGGFGEDKKCMTDKTISEIHNQPN
                     FKIRERIKDNIKRFKEDIDYIDLKVIRDTDDNLELLQTLGYSKMQISKADNIFLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFSMRKKLNDPFENLSPELKVIIKLEQRQAELES
                     GQHELEEKVNVLYDFSTIDYNQQETIRSLVNRMVIQVYQCLNFSAYEDAKLRKRVYFS
                     LWKDYRKKFGVNSYKNTAKKNYDNAIKFIENWEPNYDLSNEIYMFQKVENI"
     gene            59892..60614
                     /locus_tag="ACGI6B_000060"
     CDS             59892..60614
                     /locus_tag="ACGI6B_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9749536.1"
                     /translation="MEMNILIENKFIEKLKYTKNIPNSLYKDLRHNFLFHSNKIEGST
                     FTSESLQDLIEHNIVKGDHTLDDVITTVNSSYVFETVIDTLGEYLTEKYIKDLHGQLM
                     YNSNIHNMGLSGIYKKIPNRIIGTNSVLAQPFEVEPKMLELIEWYNNLKDITLKDLAE
                     FHYRFEIIHPFQDGNGRIGRFILLKQILENNLKLIIISSDTVDDYRQALKGCHNEYYK
                     PLEQYFESLDDYKEKNKDLWSF"
     gene            complement(60856..61155)
                     /locus_tag="ACGI6B_000061"
     CDS             complement(60856..61155)
                     /locus_tag="ACGI6B_000061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749537.1"
                     /translation="MVIKVNFGGNYSPDYIYVPNEISLKKYEIRNEFHIWIDNTEDEH
                     PFWCYENGVQIGLHYRGDAIVYWLNNYILKNANDKARLIESFSNKKLDYDLIINL"
     gene            complement(61351..61533)
                     /locus_tag="ACGI6B_000062"
     CDS             complement(61351..61533)
                     /locus_tag="ACGI6B_000062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749538.1"
                     /translation="MLKTLFILLAVFVIIKLSILIFKDALKFLNMFESKNESFIGWLF
                     LCILALVIYNLIDIIL"
     gene            61729..62301
                     /locus_tag="ACGI6B_000063"
     CDS             61729..62301
                     /locus_tag="ACGI6B_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFG9749539.1"
                     /translation="MTWNDLKNNDITYIVEYINNKLNNSKSLTKVAIGLGVSESSIRK
                     YLTKRGYKRINDEYVFIGDNSMTKVVNNRQQSNNDNTFNMTIEDDNNHNIVIDNQFKN
                     NIVSLAKDYDKIQDVLNWFENKEDTNVIEVVQDGIKIDLHSKDAIRTTVRLNKDAWNL
                     FDEFCGKFREFNKSDLMSMALLEYIKKYDK"
     gene            62434..63360
                     /locus_tag="ACGI6B_000064"
     CDS             62434..63360
                     /locus_tag="ACGI6B_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749540.1"
                     /translation="MNYNLKNENVLSELLQNEDEKTRIGILKVYKVLIFWFAKEGKLV
                     EEENDYIVEIVGNDKEEIVRDMSQNKTYNAIMAIIFKINKIFKTLNYDIHFSKPKNLV
                     AEFDLSKYLTQDEVLQVCSKLTNAQDKFIIYAAFCGLIGKDAEDLRGIKIKDIDLEKR
                     LIKLKDTEFKLDKFLFDFTKDTLEQKEYKQYLEQDSNAAEFYLFNSNSEYLIKTKPDA
                     RYGNGLKEMGYSTIRKRLLIINDYLDNKVQLNFTNLKISGIMHQMHLNEKKYTQSGYW
                     TNKSLKEFKEDNNLNFKIQEVLSIYKQKYIKE"
     gene            63365..64933
                     /locus_tag="ACGI6B_000065"
     CDS             63365..64933
                     /locus_tag="ACGI6B_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749541.1"
                     /translation="MSKDVKENISNEQKDLNKINLIYHIIYDNDRFKMITRKYFEIFR
                     LKGMSAEEISLKFEEFLYSLISDKFKKINTVNDVKKLRNQENSSSLKLKKRWSDMTLD
                     SLSEDTKSSRKKSSKYLKKELEIVESEYLKNGRIISAGVKNPKELKEQRKDFLDEWKS
                     SSSYNICDYPYLQELSTSKINSAFIHDILMCITEVLKDDYDFDINKIAIKTPPHISPG
                     IFLPFTKGKRYSHLNVKKNENEYMSDDYSAKNLSGKQMNFFYKFSIGQDENIENLKLE
                     LINPDTEKRALPKIYLDKQDLEIIRFVYTYSHMNSFSFYLGDLVKYLGLSDGKKNYIN
                     IKNRLLKLPYYTFYSNQTNDKGELKYEISFNLFSSTTIVNDENNNNRELVKITKSFIE
                     EVERVNTDIMYRNELEKLQLTQSINLAYFLEGRRSFLISCGEDIKNKSFRYDIEDLKF
                     DVHLNKSKTVKQNMEYLELGFNEIKENQFIIKDYKRGNSYFDVYFYEDFEKRKRLINN
                     TILSLPDYMIENKS"
     gene            65407..65673
                     /locus_tag="ACGI6B_000066"
     CDS             65407..65673
                     /locus_tag="ACGI6B_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HFG9749542.1"
                     /translation="MSLKYKDKEFVLVEKKNINELDNSNIKYIDLKDKQYYVVTQGKK
                     PKRFNLEDIKNIKKDLDNGISLRKCATKWNCSTRTIQDIKNNIY"
     gene            65749..66927
                     /locus_tag="ACGI6B_000067"
     CDS             65749..66927
                     /locus_tag="ACGI6B_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749543.1"
                     /translation="MQELKPVARISKSKLKDILDVFKTSIRKVEKIDYSACNSCAIKN
                     NPSFDSHKLNEFCSNCPKLKTISEFSYVTEKETELGTIEIVTNESKCMALSKSAIKQY
                     LAYHFIVSNKNYVRKAVSFQNIADICNVSIVTARHNHNVLLRVGLVYSTEIKRGKFDI
                     VIDEEYKNHLKKEDGGKGYLTMSLDTLNHLLSFENVNELKLEIKKLIWADAKGSKLGK
                     RIRFKKDNLISILPEYIKKSNKRTEAFLRSSKTLFDVSNDTIDTKRYETKETISEKYT
                     EPIRIQISKFFDNTGCSLSSNYSKVLSQINLDRYNLFEEDLRSKLDMEKDIIINDLVG
                     LSVQYGVTRVLAALDYMFNKYTVDEEDINYNEIRNPGAFIRTLIVQSINKNGSLAIAI
                     "
     gene            67536..69296
                     /locus_tag="ACGI6B_000068"
     CDS             67536..69296
                     /locus_tag="ACGI6B_000068"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HFG9749544.1"
                     /translation="MDRFEVLKYLKFNVWHSEWPLELLKGAITKDNATGNLLLQLKMC
                     NISDKEIKSAYFTIECFNDAGDLIDNNLKYVYQDINIKPHECLGDDIAIKINDNKTRA
                     INIILEQVVFSDDKVHKYEREEGLIIPKLDKIELLNIELLEELKKDYEEYDFQYEIKY
                     MPNIIEKKAWSCCCGKINKLEKDKCDNCKRSKILQIKKINKNYLESIYKKSLKKKKEI
                     EIKLEKDNRHKLKNINKNNRRTSILLISILLIFIIFIGKNIINKHAFLDEIKYKNEYS
                     KKISVEKDNVYMLKNNGKVTHRKVNGESSSIPGWENIISIASYDNSIIGIKKDGTVVS
                     KGLGNSDSDNISKWRDIIDIECDSGTFIGLKKDGTVVATGNNDYGQCNVNNWFDVVSI
                     EITKSTIEYMKNTTVIGIKKDGKVLLTGYDSLNHRKETDKWRDISKIKCGSYGIFGIK
                     NNGTVVVSGSAYDIKNWKDIVDIHIGLCGIVGIKRDGTIISTDEKFNEIIKNWKDIIS
                     AEIIDNHEGISIVGINSNGDILTSSRGQNLDNIKRLEDVEYINFDDSIDNFLVGIRKD
                     NVIFIRGINNTINEEYNEEI"
     gene            69330..69686
                     /locus_tag="ACGI6B_000069"
     CDS             69330..69686
                     /locus_tag="ACGI6B_000069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749545.1"
                     /translation="MKNYIARCLSNMGVLLAGIQVLAGIIIMCQKVEVPYDYLSDTYE
                     QYSPIMIGIGAGVIVSGIVICLVLKGLAELVENSADQTQYQKEILMLLKNNNVNEKIN
                     EVKIEQQFTNEELPKL"
     gene            69895..70608
                     /locus_tag="ACGI6B_000070"
     CDS             69895..70608
                     /locus_tag="ACGI6B_000070"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9749546.1"
                     /translation="MIITICENDDMQRDIIYDFVKIFLTKIKVKHYDILMFKNGEELL
                     EEYPNKVDLILMDINLGGRNGFDISKDIRKFDKDARIIFVTVSEKYAIKCYEINAFRY
                     MIKPIYYKEFEKYMLDFAIDWRKREAIITIKRKKQIIKIEEHNILFVEIVNRKLRVVT
                     KNEEHIINDKMDMFEEKLKEDIFFRCHKSYLINLLEVTNFNYNFVYLTSEKIKIKVAP
                     KKTKKLKEKLANFFREIIW"
     gene            complement(71072..71425)
                     /locus_tag="ACGI6B_000071"
     CDS             complement(71072..71425)
                     /locus_tag="ACGI6B_000071"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749547.1"
                     /translation="MNENLKELRIKLGLSQKEFAEKLLVSDATISRLEKGERKLTDRF
                     IFQICEVFNVNEDWLRNGNGEMFKNIDDIELAAMMGKTFSSNDEFLKKVFLTFAELTD
                     DEREVIQKVIDKLSK"
     gene            71605..71775
                     /locus_tag="ACGI6B_000072"
     CDS             71605..71775
                     /locus_tag="ACGI6B_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749548.1"
                     /translation="MYNNNDKKLINLFESFKKIDYTEQSLVYFLSLSFIVNRKCHKDA
                     NLYSRRGECNYE"
     gene            71768..72574
                     /locus_tag="ACGI6B_000073"
     CDS             71768..72574
                     /locus_tag="ACGI6B_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFG9749549.1"
                     /translation="MNNLNIKGKQKFMDLEIPIIEGGFGEGKKCITDKTISEIHNMEI
                     KHVRELINRNIRRFINAIDFIDLKVVVDNDYNLEVLELLSYRKMQISKANNIYLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFNMREQLIDSYMIQDSIERAKRWIEEEKERLLL
                     KETIQVQQPKVEYHDKVLNPDKLVTTTDIAKDLGMTAQALNKLLHLNRIIYPERINGR
                     IKCWKPYKNYLWLIPEYADFKITEHDTQLKWTEIGRKWLIEFVENIKKIS"
     gene            72638..72862
                     /locus_tag="ACGI6B_000074"
     CDS             72638..72862
                     /locus_tag="ACGI6B_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749550.1"
                     /translation="MIKQILNKRGSLLNLASEYLTEQDYLKFQGWYTHRYSKLDAYDA
                     SINEYLNYKNDKKLITYVFNLKFQEALIEI"
     gene            72886..73422
                     /locus_tag="ACGI6B_000075"
     CDS             72886..73422
                     /locus_tag="ACGI6B_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749551.1"
                     /translation="MNYNYVTKIDLKYGNTQFGFEKYSDALDKYRELKEEFKYVPCII
                     KLHSYVAHQDLYVKEIKADGFEKKYLDIKNLIKLFKEHKQNISDELSLCDKKKEEVLH
                     VIEEMNLDTASLNDKIDTIKELKNTLVKRRLVKYELNKYFAFCNTLYEIEEELKKYES
                     KYKQKENQGKSRFKKNKK"
     gene            73431..73571
                     /locus_tag="ACGI6B_000076"
     CDS             73431..73571
                     /locus_tag="ACGI6B_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749552.1"
                     /translation="MEEYHFNLIKKLCAERKIHKGWFRNIEKCGCGGKCNKKNCKMLE
                     KL"
     gene            73582..73815
                     /locus_tag="ACGI6B_000077"
     CDS             73582..73815
                     /locus_tag="ACGI6B_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749553.1"
                     /translation="MENQIELTEKDIENTLSALNIIKYICIKSKHCKNCPLGYMKNLD
                     EHNCMLYELIDKGLLPCDWSVKKVTRLLDTTEE"
     gene            73834..74247
                     /locus_tag="ACGI6B_000078"
     CDS             73834..74247
                     /locus_tag="ACGI6B_000078"
                     /inference="COORDINATES: protein motif:HMM:NF021182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFG9749554.1"
                     /translation="MELDFRIWNGFRMIHGEELRLLTEEMFIKSGYSEQYYNDICIPR
                     VEDLKIMQYIGLKNCFEKKIFQYDIVKFIFDGVWKVCLVNLSLPKGATVTFESIGGKY
                     TFNLSEIFSKDRKLEIIGNFYENKDVIKKYNLSIE"
     gene            74257..74451
                     /locus_tag="ACGI6B_000079"
     CDS             74257..74451
                     /locus_tag="ACGI6B_000079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749555.1"
                     /translation="MDEMIIRVKMGEPLPTLGKIIKCTTCLTNEEKCFKVNKICKLSW
                     INDRNSEGLIVECKGKYIKY"
     gene            74601..75212
                     /locus_tag="ACGI6B_000080"
     CDS             74601..75212
                     /locus_tag="ACGI6B_000080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFG9749556.1"
                     /translation="MARCIDSAEILAKEDEVKSMSKKDKLSIFISLILRHKPETIGIK
                     LDDYGYADVNELIEKINNTGRNINIEILEQIVKEDNKQRYSFNDDRSKIRANQGHSIN
                     VEVELKELEPPKCLYHGTATRFLENIKKEGIIRQSRLYVHLSNDIDTAVKVGKRHGVP
                     VVLKINTGKMYENGYKFYLSENNIWLCKYIPFEYVEKISRVVK"
     gene            75239..75565
                     /locus_tag="ACGI6B_000081"
     CDS             75239..75565
                     /locus_tag="ACGI6B_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749557.1"
                     /translation="MDFNFRKICEKDELTDDFIIKICQLSHEEFGGTIYVPCNIHPCG
                     WDELEVPNPKDLDSDKLMEEFLLEYKFECENISSNIYDSDHPFIGKTLREILMIVDCR
                     FIIGVI"
     gene            75580..76356
                     /locus_tag="ACGI6B_000082"
     CDS             75580..76356
                     /locus_tag="ACGI6B_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749558.1"
                     /translation="MENIELTPRDYFEDIKNKKQKNTNENLDIVYENSLILLNKYIVT
                     GQKKGAKKLMFLLECIEKERNVIELGIDTFVYKEDIEKYIDDISKNTVKIIELENYER
                     EIPDEIVEIIEKTKDIFDKMYVLFTDYTGKITKEISKERRDKDPILFGVFSDEKSRAI
                     VDRFYYLGDWEDEYCDLTLEKMINDYKKKGRQDILKHIITPKSLDELKKELDSLEKNN
                     DTYKQVANIKPLPTTKNSSVEPPKLNIFKKIKNRVFKNEK"
     gene            76346..76747
                     /locus_tag="ACGI6B_000083"
     CDS             76346..76747
                     /locus_tag="ACGI6B_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749559.1"
                     /translation="MKSNVDLTENRDFRDVRGRGINILGIEDAPNRMLSFSDFGIGRL
                     YGKNLEFEFNKNEVFLTGNANDREEKKFLILCEENKVCVRCGDELREGWDKVSIELCN
                     ECYEMLDVEYKNKKIIRELFFRDRDVNIELL"
     gene            77041..77256
                     /locus_tag="ACGI6B_000084"
     CDS             77041..77256
                     /locus_tag="ACGI6B_000084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749560.1"
                     /translation="MCKYCGQIVAGSTPVYHPNMKVNEYCVKCEFSACANNYEGYCNS
                     KDKEIEFKSICLKGNKEILQCKNFDLE"
     gene            77287..77472
                     /locus_tag="ACGI6B_000085"
     CDS             77287..77472
                     /locus_tag="ACGI6B_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749561.1"
                     /translation="MKINVHVKTNKIGSDCELEFEIDDDDLKDMSDEEREGYIDKIAW
                     DYVFENFIDWNWYEVED"
     gene            77527..77928
                     /locus_tag="ACGI6B_000086"
     CDS             77527..77928
                     /locus_tag="ACGI6B_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFG9749562.1"
                     /translation="MKELLEVKKIFDSLASTSSRKEKERILEKYKNNRMFVECLQFLL
                     DSNILTGISKNKICKNLNNTGHNELENIYDMLDYLIKNNTGRNIDVKTIQVFASKDEK
                     LKDFIFNLATKSIKLGITYKTVDKIMPGLII"
     gene            78030..78533
                     /locus_tag="ACGI6B_000087"
     CDS             78030..78533
                     /locus_tag="ACGI6B_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749563.1"
                     /translation="MTDKIIIASVDGEEMIFRTEMKKLEKGDSLIVSVNGVKRLALFI
                     RYISTCEEYIEDIAFQKVDEILYENTIAKEKERVKKVFKDKCMKNYKPIVNTEIDSYL
                     RECGFNCDGRQFFKSIDNTQILISRDCDKDVLTVSKGLTITKTDITNKNLITVIKIIN
                     NSLLIVR"
     gene            78593..80818
                     /locus_tag="ACGI6B_000088"
     CDS             78593..80818
                     /locus_tag="ACGI6B_000088"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HFG9749564.1"
                     /translation="MKNVIKRDGRIVPFDEKKIINAIEKSFLSLRNKGKSEVFDRELA
                     TLIAKKIKNKPCEKMHIEDIQDIIEFSLMASKKKDVAKEYITYREKRNIARGRKTYND
                     YMSIVNTESNDITKENANMNADTPAGMMMKFASEATKTFTDHVLLSEEAKEAMMDNYI
                     HIHDKDYYPTKSLTCIQHPLDKIFNNGFRAGHGSSRPTKRIETSSILGCISLETVQNE
                     MHGGQAIPAFDFYNAPSVRKTFIEEIKNIEIKDDIDLSHLYNIEIEDYIIKDLKELDS
                     EKRNVQHAINRTVWRVHQSMESFIHNMNTIHSRGGNQVVFSSINYGTDTSAEGRCIIR
                     EILKSTYEGVGNGETPIFPIQIWKVKEGVNYNPTDRNYDLYELACKVSAKRFFPNFLN
                     LDATFNKHVKWKMCDPNRYIYEVATMGCRTRVFEDLHGEKTSIGRGNLSFTTLNLVKM
                     ALEAKEKYPNNEANRVAEFIFLLDKYSDVVARQLYDRYLFQCTAKAKQFPLLMSGLWK
                     NSENLNPNDDVSEVLKHGTLGVGFIGLAECLIALIGEHHGESEKAQKLGLEIISLLKS
                     KVEKYKKKYNLNYSVLGTPAEGLSGKFTQKDKKIFGEIKNITDKDYYTNSSHVPVWYN
                     CNVEHKAKIEAPYHELEGGGHIFYVELDGDATHNPKAIMKVVDLMKKYNMGYGSVNHT
                     RSRCMNCGFENADSELKECPKCGSNSINIIQRITGYLVGTTDSWNNAKLAELKDRVTH
                     G"
     gene            80811..81308
                     /gene="nrdG"
                     /locus_tag="ACGI6B_000089"
     CDS             80811..81308
                     /gene="nrdG"
                     /locus_tag="ACGI6B_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFG9749565.1"
                     /translation="MDKDYIYILDIKHDTIVDGEGFRTSIYCSGCNHMCRGCHNPQSW
                     DIKNGTLTKVLDIYKEIISNKFSDVTFSGGDPLLQLQGFVHLAKLIKKNTNKTIWCYT
                     GFKFEELITDNEKLELLKLIDVLVDGKFEEDKKSLELIFKGSSNQRIIDVQKTLYEKQ
                     IVLYE"
     gene            81343..81474
                     /locus_tag="ACGI6B_000090"
     CDS             81343..81474
                     /locus_tag="ACGI6B_000090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749566.1"
                     /translation="MTKEEFITKFLKEQQRRIKVLYYEVENFKMDRRFNERKKSRRH"
     gene            81509..81916
                     /locus_tag="ACGI6B_000091"
     CDS             81509..81916
                     /locus_tag="ACGI6B_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749567.1"
                     /translation="MYFETDIIKNKNTVNKIIKYCGGNTEKLKDWLKEIENKSISSLE
                     ELKEYPLKRNYKFVFSKEEAPNLDKLIKMKLRDNCLEKCEICSLENLLSMLNDEILDL
                     NKEDISYEDNIEWIENCKEELIKLKVGSICIDW"
     gene            81937..82254
                     /locus_tag="ACGI6B_000092"
     CDS             81937..82254
                     /locus_tag="ACGI6B_000092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749568.1"
                     /translation="MRFWCENCNKYFNAEETLQEYNYFLNEEILICPSCKKDLIPIAR
                     ETELSLGFDSDTNQLTYVEYDSSDYSLLRKVNADVEDVVKPIIHYIRNLNKNSLDLNG
                     ITE"
     gene            82339..82689
                     /gene="tnpA"
                     /locus_tag="ACGI6B_000093"
                     /pseudo
     CDS             82339..82689
                     /gene="tnpA"
                     /locus_tag="ACGI6B_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            82707..84146
                     /locus_tag="ACGI6B_000094"
     CDS             82707..84146
                     /locus_tag="ACGI6B_000094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9749569.1"
                     /translation="MSNYILTLQLKTEKFQEDIICNAFDKYRKVYNSCLSELFKRYNH
                     MIESKEHQANCKYKGKDRNKIFSEINKKYNLTEYSLHKFVKFIGKYYKLHSAITQKIA
                     TRCFNAFNKYIYHQAKRVNYIRYNELTSIEGKQNSTGISYKDGIIKFNKMTIPVIIKN
                     NDNYAQRAIQDKIKYCRILKKEIKGKIKWYVQLVLEGIPPKKTTNQGKIKGQIGLGNV
                     GIDIGTQTIAISSKCDVKLLELAPNVNNIDRQIKLIQRKMDRSKRATNLNKFNDNGTI
                     KQGNRDKWIFSNHYLKLKRLRKELYRKQAEIRKQDHYILINKLLNLGNKFYVETMSYK
                     RLQTRVKDTTINEKTGRINKKKRFGKSLANKAPSMFLTMLDNKLKYNNERLYKIDTAK
                     CRASQYNHFSDEYNKKELKDRWNNGMDIQRDCYSAFLIMNVNEDLKSINRELCVKTYD
                     NFKILHDKEINRLKKLKLNGYKLISSMGI"
     gene            84347..84571
                     /locus_tag="ACGI6B_000095"
     CDS             84347..84571
                     /locus_tag="ACGI6B_000095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749570.1"
                     /translation="MVIYKNKKKDIIRDESMYDYGMSLSSIMKVILNHQEDIEKLKEE
                     EVAEFIKNKLENEHDCDELKIISEVFNEIY"
     gene            84558..84668
                     /locus_tag="ACGI6B_000096"
     CDS             84558..84668
                     /locus_tag="ACGI6B_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFG9749571.1"
                     /translation="MKYIKHVDNGVMRAIGIDNYKPLLNIIKEKVNLESN"
     gene            84697..85164
                     /locus_tag="ACGI6B_000097"
     CDS             84697..85164
                     /locus_tag="ACGI6B_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749572.1"
                     /translation="MGLDLGFYSVKTKVTDLDIYLNKTRGEKYKELNNDEEEFYKFEE
                     LLDIDISLEDWSVIRMIYKAKWEKGYESYEDEAILITKEDLEDYIIPFLKQKDINSKI
                     YNKDKEYYYKNDKKVTVDDRYTQEFWDLVLKEFQKMLNIIDFEKHSLIIMYWY"
     gene            85200..85580
                     /locus_tag="ACGI6B_000098"
     CDS             85200..85580
                     /locus_tag="ACGI6B_000098"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFG9749573.1"
                     /translation="MKKMYIAINSDLNMSPGKVGAQVAHAVYDYLYNKILDTVSCSYE
                     TEIIAHELIDLKCDLTSFKNNGDTICILKAKEAQLLKFKEKGYLTIVDRGLTEIPKNS
                     ITCVNLGIFNEDEIPSTIKRLRLY"
     gene            85596..85751
                     /locus_tag="ACGI6B_000099"
     CDS             85596..85751
                     /locus_tag="ACGI6B_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749574.1"
                     /translation="MYSKGDVVKVWCYQCERIEFQEFVGYFNSYFDNYKLKKRWKCSK
                     CDVINNF"
     gene            85778..86224
                     /locus_tag="ACGI6B_000100"
     CDS             85778..86224
                     /locus_tag="ACGI6B_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749575.1"
                     /translation="MKIKKIILGLDNLENVELGIGDVGGFYLDNIHEIIGGFALSYIG
                     KCKKADYLHIQIHKRVDEIQRLHDYKDITNVKIEYEDGSVEDIYVPYKQEGDFLGLDN
                     LYQTSFISKQGYLHIVISKDKTVEEVFNEEFEDDEGVEFSFNMYGI"
     gene            86502..86666
                     /locus_tag="ACGI6B_000101"
     CDS             86502..86666
                     /locus_tag="ACGI6B_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749576.1"
                     /translation="MTDKEMEEYIINLIKDKIKEEYGENILPEILMEKFIKLERKGSV
                     TVDVRDLIEG"
     gene            86710..87963
                     /locus_tag="ACGI6B_000102"
     CDS             86710..87963
                     /locus_tag="ACGI6B_000102"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFG9749577.1"
                     /translation="MEVQGKYNKAKIFTENVDSITIGQIINLCNQKEFKESSIRIMPD
                     CHAGKGCTIGTTMTISDKVVPNLVGVDIGCGMATIPLEKYINNINYTKLDKIIRDYIP
                     HGFKIHNKSREKLLKNLFNIDINNLRCEVNKNRAYMSLGTLGGGNHFIEVDKSEKGQM
                     YLTVHTGSRNLGKQIADYYQDKAIKYCIEKYKKSYDDAKEFLISTLKKDNKEHLINKY
                     LKDLSDRKLNKPHDDLCYLENDLMEDYLHDMSIAQKYAAANRMVIIADILFKYNSLEV
                     NYDHFIRDIKCDVIECIHNYIDMDSKILRKGAISANKDETVIIPVNMRDGIILGKGKG
                     NSEWNYSAPHGAGRILSRGKAKEKISLDEFEESMKEIFTTCVGQSTLDEAPQAYKPIE
                     DILNNIGDTIEIMEVLKPIYNFKSN"
     gene            88005..88907
                     /locus_tag="ACGI6B_000103"
     CDS             88005..88907
                     /locus_tag="ACGI6B_000103"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFG9749578.1"
                     /translation="MIKLITHNDLDGVSCCIIAKYILREQIDVNYCNYKNINDVVLDT
                     LKNHDKYEQILITDISVNEDVAKKLDRIKNKVQLLDHHPTALFLNNYDWANVEIECNK
                     GKTCGTQMLFNYLQRYYDDTSESTLKYFIELVRRYDTWEWKDKYNDLDAKYLNDLMYI
                     MGIEDFIMDMLTKIDSDEMCLFDKLSLKLLEIKQKEIDRYVEKKDENIMVKEILGYNA
                     GIVFAENHISELGNKLSELHPELDFIVIINREIISYRTIKDNINLSEIAKVFGGGGNP
                     KASGSQIDESCIDNYIKDIFNIKS"
     gene            89015..89293
                     /locus_tag="ACGI6B_000104"
     CDS             89015..89293
                     /locus_tag="ACGI6B_000104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749579.1"
                     /translation="MMIDKFNYELYNEFPHLSPIIELYNLNIEYIREVVSILINTDMT
                     FQRFKYSKSRVECSMKAYIISPNSKLSVVFAYDCELNGIDLDNLKAII"
     gene            89340..89750
                     /locus_tag="ACGI6B_000105"
     CDS             89340..89750
                     /locus_tag="ACGI6B_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749580.1"
                     /translation="MCKFCESIIDNTKDILLTSRSRLLEDNTCEIIAEDNCNNCYDGC
                     YEYFKLNGYKIRGNTYVNVDYYKKIRKIIIAPCSESVHINYCPYCGKQVSKDIKDFNC
                     IPEHIVDVRYKNGDVYDYDMEKSVQEVMDNANNN"
     gene            89781..89954
                     /locus_tag="ACGI6B_000106"
     CDS             89781..89954
                     /locus_tag="ACGI6B_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749581.1"
                     /translation="MNLWSKIEEPVMRAVSGIVFNIWGNVCFSIYYIEENKQIELEIY
                     DRPYRYEGKKNSC"
     gene            90064..90207
                     /locus_tag="ACGI6B_000107"
     CDS             90064..90207
                     /locus_tag="ACGI6B_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749582.1"
                     /translation="MNKGIKNYITAVNYLLDNLEYMMEDDEFNNEKLQKRIDVIRKFE
                     REL"
     gene            90209..90514
                     /locus_tag="ACGI6B_000108"
     CDS             90209..90514
                     /locus_tag="ACGI6B_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749583.1"
                     /translation="MGKIKNGEIDTEDYRLFKNIQKEKDRVYSKYINEEYIMEEFINY
                     LRLEDKDYTTEILERYARYIDENLDNRCPYCVYNICSEHDNCFDGILENLKEKIKIK"
     gene            90672..90872
                     /locus_tag="ACGI6B_000109"
     CDS             90672..90872
                     /locus_tag="ACGI6B_000109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749584.1"
                     /translation="MIKERFKAKDSFNMDEFTKIWLMDFKEDFDLSLKKMIENTIKKS
                     IKDTYEETERAITNIENLINKL"
     gene            91077..91361
                     /locus_tag="ACGI6B_000110"
     CDS             91077..91361
                     /locus_tag="ACGI6B_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HFG9749585.1"
                     /translation="MKKFLLTGLLGTMLIAMSGCTGIQSSMKDAESDVSGLNRTVNVY
                     SDNGEVLKTYTSKSMRVKDGDGGTITLDFDGKRVIICNAHVVIEENSSNK"
     gene            91373..91540
                     /locus_tag="ACGI6B_000111"
     CDS             91373..91540
                     /locus_tag="ACGI6B_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749586.1"
                     /translation="MFKILKTILIIIWVVDILNFPQFQFLDTTYPINTLAWLLIWILI
                     PSSSIYIDKKE"
     gene            91558..91692
                     /locus_tag="ACGI6B_000112"
     CDS             91558..91692
                     /locus_tag="ACGI6B_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749587.1"
                     /translation="MILNIATIFNLGCMVANYLQDNTNGILLNGFCLIALLLLNNDLD
                     "
     gene            91719..91871
                     /locus_tag="ACGI6B_000113"
     CDS             91719..91871
                     /locus_tag="ACGI6B_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749588.1"
                     /translation="MKITISIEEAMDIIKQHYENKGIEIKDLSFNLEKTGMVRNIKFK
                     GEIILK"
     gene            92082..92504
                     /locus_tag="ACGI6B_000114"
     CDS             92082..92504
                     /locus_tag="ACGI6B_000114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9749589.1"
                     /translation="MSYPTAIQEFSNQEISIDTVDIRDDSRANIKQDYLKFQPKEKYD
                     VIITNPPFNLSLDIINKALNDVREGGFVIMLLRLNYLGGKVRQRLWGNNMPKYIFVHN
                     RRMSFTDDGKTDSIEYAHFVWQKGYNPKFSQLKVLISQ"
     gene            92530..92742
                     /locus_tag="ACGI6B_000115"
     CDS             92530..92742
                     /locus_tag="ACGI6B_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749590.1"
                     /translation="MEINRRRCEYAARTGRYPNSLILWEGYYSLLNIQYIEDKTRGYI
                     TKFNGMDVIKCNDFLKIELFEKNINN"
     gene            92771..95842
                     /locus_tag="ACGI6B_000116"
     CDS             92771..95842
                     /locus_tag="ACGI6B_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9749591.1"
                     /translation="MRYNNYHSHKIYSNIRSLDVITKPQQYIDRAIKLGQNTYFTTEH
                     GYQGNVYEAKTLCDEYKLKMIIGAEFYYVNDINEKDRGNYHLIVIAKNNDGYKQINKA
                     LSLANKNGYYYKPRIDEKILFEIFKPGDVVITTACVAGILKLENREELILKLKNYFKN
                     NFFLEVQSHPHKTQALHNKDVLELSNKYNIDIIHANDSHYIYPEESKYRTKFLKAKGI
                     NYPEEDGFILDYPNSEDIFKRYEKQNVLTKQEVERALKNTLIFDECEEITLINDDIKL
                     PSISKNPNKELKEILNKEWLEKRKNIPKNRWNEYLDAIRYEFDIIEKTHMEDYFIIDY
                     KIVQRAKQEYNGLLTKTGRGSAPSFIITNLLGLTEIDRLNAPVPLFPTRFMSVERILS
                     AKSLPDIDLNAEDAEPFIQSTRDLLGEENCAWMISYKPLQDASAFRLWCKANDMKLSE
                     YDEVAKNLDKYTNDVFWKDVIKESKVFVGVVESVSFSPCSMLIYDRPIDEEVGLLKTK
                     DGVCCNIDGYYCDKYKYLKNDYLTVKVWSLIRKICELANISIPTIEELNILLDAKTYD
                     IYKDKLTCTINQVDSDYATNLASKYKISSVAETSAFVASIRPGFASLLDNFINRESYT
                     TNVKELDILLEDSYHYLMYQESIMKYLIWLGIEESESYDIINKIKKKRFKEKELKELH
                     IKLKNNWIKKVGTENNFEDTWQVVNDAASYSFNASHSLSYAYDSLYCAYLKSHYPLEY
                     YTVAFNLYNEDTERTRKLTDEIEYFSIKLENPKFRFSKSEYFFNRDTNSIYKGIESIK
                     FLNSDIGEYLYSLKDNKYDSFLELLIDLQGHINSKQLSILIKLDFFEEFGKSNKLLET
                     YDIYNSIYSKKQFKRDNLPCDINTMRKYSNKETEKIFKEVDTKELCSYLESQIENTDI
                     PTNEKIQAHFEFVGSCNIKDSNSNPRYCLVIDIDTKYSPKVTLYNISSGNIKIFKVSK
                     PIFEDNKIDVYDLVYLKSIKEKAKSKKVDGKWIRSNTETEWWIQEYWKIEN"
     gene            95945..96196
                     /locus_tag="ACGI6B_000117"
     CDS             95945..96196
                     /locus_tag="ACGI6B_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749592.1"
                     /translation="MISILIDEQRYYSLLEEKKDLSMRIDELKEENAFLKQENEEYEE
                     ILNEWECVRKEIVATCKKKEILGEKPSELEMVLQKIEDI"
     gene            96196..96516
                     /locus_tag="ACGI6B_000118"
     CDS             96196..96516
                     /locus_tag="ACGI6B_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749593.1"
                     /translation="MSVNKIYEKKEYIIIKSKSGYIVYNTNKEFKEGHTHLKSYNAAK
                     TAIDLVIKRRLPKSYSIYFLTSLMRISNDVQYIKHISDIVYIRNNKGLKEKYINSKNI
                     KKNY"
     gene            96591..97712
                     /locus_tag="ACGI6B_000119"
     CDS             96591..97712
                     /locus_tag="ACGI6B_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HFG9749594.1"
                     /translation="MKSYKEKQFLVFEFEDGKNVKYNLATGEYVGKSGRIVKDVCTQL
                     RGYNLHEVINSFEDENYKCFLNFVDGRVNRSKSSRAWGRRVERIRNIGSFLNEINNYS
                     EYEQLFSAGLKKIIYPIHCDIKSIPKGLISICRNYSITLSDRLIQGYSKNPNLYNNLL
                     SLELNSIRKTNLISILDSGYDRMIFDRLIDIYKYKPQSLINYIDNLMTYEALNGLEST
                     LNELYDYVVMMSVISDKYEKYPRHFLTTHKIASRNYNRLKTQFEEEVFNKRIDKSLEY
                     SYQDYKFIYPKNTNEIKDEAVQQNNCVSSYIQNVIDGKCHILFMRKKDNPSKSLVTIE
                     VRNGKIVQAKGKYNRDVNEREQEAIDKYNNRIERMKKIC"
     gene            97706..98149
                     /locus_tag="ACGI6B_000120"
     CDS             97706..98149
                     /locus_tag="ACGI6B_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749595.1"
                     /translation="MLIEGTKIRLMKEIQGFEMLKIGDIFVITSVGNNGAIHFKTNYG
                     MGFMNYSELKKYFEVAIETKKKEHIWSEWRTATVFGTNKTCTYRTNGKKVEVRMGDFK
                     ASATCHDSDEFELNKGVELCLARIEVKKANKQVNLVLDKINNKEI"
     gene            98159..98314
                     /locus_tag="ACGI6B_000121"
     CDS             98159..98314
                     /locus_tag="ACGI6B_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749596.1"
                     /translation="MSCKFKERCENKNRDCDYCCYNPNAYLEDAFEWNGEGRKPTDEE
                     LDDALEE"
     gene            98358..98918
                     /locus_tag="ACGI6B_000122"
     CDS             98358..98918
                     /locus_tag="ACGI6B_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749597.1"
                     /translation="MRWRNILVTSVLVMGSSIHIFANTKEMSTSRMDKNIVADTAKNI
                     NLMYKYEWEQHKNSSEYKLEIKRINLEKDLGIKIEKLIPVTWEVSYYTSLNCENSKYG
                     AITATGEKLQYGFVANNYLKFGTKILVDGNLKVVKDRGSNKYFGNSNAIDVFVPKLEG
                     ESDYKYYKRVNNMGRHYKEGYIIVEG"
     gene            98930..99598
                     /locus_tag="ACGI6B_000123"
     CDS             98930..99598
                     /locus_tag="ACGI6B_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749598.1"
                     /translation="MNVNGIKIPGIIDVSNVYVYGIKESIIASGYPMYEQQVIDMKKI
                     NISEKDLKRANQLGKSKQGSGHDCFSKGIIIQMDLQWSEYMWRQWDRYHHNDYVSSQS
                     KMHRLSKFNLDDMCNKYVDKNTINFLNELIHEYNNFEELKLNNSNNIILRNGEKVEVT
                     KETLWKIIISNCPSGLMLTARVTLNYLQIKSMINQRENHKMEEWRILCDFFKTLPKYK
                     ELMM"
     gene            99644..100537
                     /locus_tag="ACGI6B_000124"
     CDS             99644..100537
                     /locus_tag="ACGI6B_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749599.1"
                     /translation="MAEKRINYNQVFVSGEVLDILNIARMNQGTGQEAIRFTLKVETA
                     PNESVNVDYFTSMFKSDGSPNQMFLGIETIANEIKTRAEDGKGDIVRCVCSLDNNLYY
                     KDGEKKERFQISGTFCNREKYDDKGNPIDDKGEVVKSIKASQVWRVYTLIENIEEKED
                     DLGKYLEIKGLINKYGKQGCNMTFRIHNEDMIEGFKSLYKIGDVGLLEGTVKSMVVSK
                     SAGFGSRIKKSAFTFLEIEGGDEPLKDGDKIFSDKNYPFTDKNIEAMREKIKEKDKKE
                     KQRDIDKNGKTIEIRDDDVPF"
     gene            100577..101134
                     /locus_tag="ACGI6B_000125"
     CDS             100577..101134
                     /locus_tag="ACGI6B_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFG9749600.1"
                     /translation="MEVKEKTMNDGFKKVVNEEIKTYISNLYSDRIDGLKESDSQIEI
                     TKALIAFHLDIESIPKDNTNPFFRSKYANLDTILKAVNPLLAKHDLILMQSAEDAGND
                     KVYIKTKLKHSSGEYIESNSAPFKPAKTNDIQARGALETYLRRYAVQSVLALSFEEDD
                     DGNSLTNKSKGKSEEENSTIKKSRV"
     gene            101195..101746
                     /locus_tag="ACGI6B_000126"
     CDS             101195..101746
                     /locus_tag="ACGI6B_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749601.1"
                     /translation="MNCKCRFCKKKLNTNDAYKVEHITSGGNKQNRYYCNEQEYRKEQ
                     QDIYFWKQCQLGIDYIMGYTVINNQKNKMLQEIIKGGYTREELYDCIIEKKDEVIELL
                     NYRKDIEEEYPKLCYVFTILKGSIRDITIKNKQMKYEKENERIYKEVEKSEEYYEIIA
                     PKKVIYNKRQSLFDKVKGVYQDE"
     gene            101802..103313
                     /locus_tag="ACGI6B_000127"
     CDS             101802..103313
                     /locus_tag="ACGI6B_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFG9749602.1"
                     /translation="MLKSLTLFSEYKISESDFIIDKVKFFFSLGRIMSKTHNELDEIS
                     VAKFVSSNKLKSEYERYGGWNSLSSAMEYGKETNIAAYIDDLAKNNLLIALDKRGFNV
                     IKEMEHNGLKFIPFEIFQSMKCREVEEFYEGLISSCSVNSIKNNMKVENLLLTKDDRE
                     KLKQKTEAGTPYNIIFEYTEKEIGLSNNEESKYIYSLPILSNRTNGIGKGGGINIIAG
                     FSGIGKTTLLFFNYILAMIYRGEKIVIFANEQKSQYFKSMLVSFIAYNIFNYHDLDRN
                     KIDNGDFTDFEEDLMEKIEKFLRDRCFAENLKFIYMEEFEISEILRKSKELVTHQGFT
                     GIAVDTFKSEDSSDAHYTGKLIENSKLLDSFGNKYNVITMLSMQLLTAQENKSSYLSA
                     GDLSEAKAVKNVCGLLKLMRKVVNELELDSTNKKFFLKPYKLKYNKLKKTTEREYIQF
                     DAKDLQKEYRLLFLNKSRRGQDGDVILLRFYGKTGRLEEIGRCEKVYRGQLSY"
     gene            103323..104384
                     /locus_tag="ACGI6B_000128"
     CDS             103323..104384
                     /locus_tag="ACGI6B_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HFG9749603.1"
                     /translation="MEIKDLTNEQVINFMEYLGSDLSPKSNDRQLIFNTCLCHNGDSY
                     KLFYYTESKTFHCYSSCGHIGSLIDLLIHINKYEFKDAINEIKDFFGISNQPMLRKGF
                     RIKKKVEQIHDIKDIQIELLPTPKKPYVYKTFQQVPIEEWENENISFEVLKTYQVYYN
                     LYENQIVIPHFCWHDRARPVGIRVRNLDEDKAKSFGKYIPLWYDNRCYNHRLSLNLYG
                     LNVNKKSIKNSKKVIVFEGEKSVLQMATMYKNNPSVAICGSNFSREQKKILIDLGIEE
                     LIIAFDRQFKVKDDEEYVIWRDKIYKLVQDIKDVVNISVIWDKYNLLGYKDSPSDKGK
                     TIFEKLLKNRINIENFVKQ"
     gene            104489..104761
                     /locus_tag="ACGI6B_000129"
     CDS             104489..104761
                     /locus_tag="ACGI6B_000129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749604.1"
                     /translation="MIKFLIKNVHMGDKLYCINNIGNYKKGEIYTVSDTSFFGLFHKT
                     KGENCLPIECYFIHLPKKEELESNPKSMTKEEIEDELGYKIKIVSK"
     gene            104782..105633
                     /locus_tag="ACGI6B_000130"
     CDS             104782..105633
                     /locus_tag="ACGI6B_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749605.1"
                     /translation="MSIFNKKYGRNVKITEEEPPSPPPKQPIYNRDNIQYDKEMERKL
                     LDIDRLKIELEKEKKIEEERKKVERVKENEGKEWAWVEGYKCTDKNMKCKEFQYELNK
                     AYSMDGDDINIWNWGFHLCLDLGDVFQYYAPNLSNRFFKVKALVKKADKYKYGKEYGE
                     VTLLSGSVLRGHKINKIAAKEIIFLEELTFDKLKLHIQNSFPEVNTKSKWYAICKLGE
                     QEYYHRMFMNQMKKIRFSDTFSQLLFDECRNYCEILEILNKAIAYSKENLSKDMIIYL
                     LMKDIRK"
     gene            105657..106100
                     /locus_tag="ACGI6B_000131"
     CDS             105657..106100
                     /locus_tag="ACGI6B_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749606.1"
                     /translation="MDKINKIDLIKLKEMNIYDNYQLKKIASKMILHLRYEIMDFCGC
                     GSPEDISFMIKGVLTAIQNKEKNCNLEYTERCYIFEIEFNNVCGIIGSKNDLIQEFIL
                     NALNSYGLLEHGSSIWGSWLSDYGKQILCAFEIVGDCILDVSYLD"
     gene            106142..106786
                     /locus_tag="ACGI6B_000132"
     CDS             106142..106786
                     /locus_tag="ACGI6B_000132"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HFG9749607.1"
                     /translation="MSDLHGMYDKFISMLEQINFNSNDHLYILGDVLDRGDKSLEIID
                     YIRKYKNITLLKGNHELMFQESYTDVNSSFLWFYNGGKDTFYDLGMKSYEYKENFYKY
                     IKNLPYLEIIDNFILVHAGLYLPNNYENLSIEQIIELQEEDICVWDRTILNTNKHIKG
                     YTIIIGHTPTQNIKDYEKAEIYKQEGIINIDCGACFDNGKLACLRLDDMKEFYI"
     gene            106797..107324
                     /gene="yyaC"
                     /locus_tag="ACGI6B_000133"
     CDS             106797..107324
                     /gene="yyaC"
                     /locus_tag="ACGI6B_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HFG9749608.1"
                     /translation="MDILKISYNNLNAISKISEVLRDIVNEDTLIVCIGSDRVLADSL
                     APMIGSILEKSTIRNKIFGVLGDSIHALNLEQKIQAIKNNYPNSNIIAIDACISKISD
                     KGTIIISNKPVKPGLGVDKKLLEVGDYSIVGIIGRNKYDIYDTSDPELILDLADVISK
                     SLISILLEKEERMIV"
     gene            107321..107575
                     /locus_tag="ACGI6B_000134"
     CDS             107321..107575
                     /locus_tag="ACGI6B_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749609.1"
                     /translation="MIQEVRQEILLKNGLILYTGDLVEIKYKSDEDVIEHTCKGKIKE
                     AKELFIKLDTSKKYKTSERMIYSWELKEIKKVDDEDEKDN"
     gene            107559..107816
                     /locus_tag="ACGI6B_000135"
     CDS             107559..107816
                     /locus_tag="ACGI6B_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749610.1"
                     /translation="MKKIINSKYRALYLFIFFLIFNLIYSLYFGRNTEIGFYSSTSCI
                     EEYILDIITSIGLFFSMMLAGFDVTENFINSLRDYCEEDAK"
     gene            107818..108036
                     /locus_tag="ACGI6B_000136"
     CDS             107818..108036
                     /locus_tag="ACGI6B_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749611.1"
                     /translation="MNKLELKDYISKVELNDMPLTLDNKYMLNEISISEDSKIFAVKD
                     TSGITSYYTEIEFNKLFRGINSILENII"
     gene            108111..108950
                     /locus_tag="ACGI6B_000137"
     CDS             108111..108950
                     /locus_tag="ACGI6B_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFG9749612.1"
                     /translation="MEKELYSFSKLNAFNTCPFQYYLTYVKNLNREQSCYGYYGNELH
                     SLLEQLQQRKITNQEAIQKYNEVIEYANLMDYNFPTPNSRINYLECILHYIENFVPIE
                     CDRYYIEEYFEFDINGITMRGYIDLYYIIGNKIYVIDYKSSSKFSKKDLPKKSMQLIL
                     YAMYLKEKYPDKIIEYVAFDMCKYMNNEKGVLIERNKIDSINDYERAIVKIKYTKELE
                     KQLIDFVVDTVTQIKQLDSNDESVWCKSDDKSNQFFCKTLCSHYNKGCKHKENKSFRK
                     SKS"
     gene            108988..109308
                     /locus_tag="ACGI6B_000138"
     CDS             108988..109308
                     /locus_tag="ACGI6B_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749613.1"
                     /translation="MNIPKQVKVGGLFYKIEETEEPILVNNQLCYGLADYGTETIKLS
                     NNLQSERVKEATFLHELFHCIFRDRCIESEDEEYLVDVLSKGLHQVIIDNPNIFELKV
                     SIDG"
     gene            109301..109882
                     /locus_tag="ACGI6B_000139"
     CDS             109301..109882
                     /locus_tag="ACGI6B_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFG9749614.1"
                     /translation="MDKLKNIFVFLGYSGSGKDSIVTEISKQFNIPILISHTTRPPRN
                     YLEIINKTYYFVDNKFFKKEKNNFIEMRKYIVHDGSTWLYGIHKSELENKKYALTIVD
                     ASGYKALEKYFTGTKTKLVPFFINADEDTIRKRLIDRGDNLKEIERRLVDDKIKFKEF
                     LNSENYIVIANNLSITNAVEQVKIHMKEGIQWS"
     gene            109873..110367
                     /locus_tag="ACGI6B_000140"
     CDS             109873..110367
                     /locus_tag="ACGI6B_000140"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFG9749615.1"
                     /translation="MVLALDVSMSSTGWAVINRNKNILKYGKIVTKKDKFKSEDERMC
                     YICNAIQELIITHNIQIVLIEDQFTSRNSKTILSLRKLLGAIMRTVKLNNIEIEYMYP
                     VSIRKYLLNNGKAKKEEVAAYVRENIIDIGEYIDRTCKAKTSDIYDAIALGIAYLNKL
                     DTNR"
     gene            110391..110894
                     /locus_tag="ACGI6B_000141"
     CDS             110391..110894
                     /locus_tag="ACGI6B_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFG9749616.1"
                     /translation="MSINVKIKKLNQNAIVPSYAKEGDSGIDLYTLKDTVIPAKSAVA
                     ISTGISLEIPYGYEMQVRPRSGISLKGMFFTRYVTIYDKVQPKKQIRKIFVAHPTVRL
                     GTVDSGYRGEIKIITYNEEDFDILIPKHTKLAQGVFQQVPRAILKVVDELTSSERGSN
                     GFGSTGI"
     gene            110895..111035
                     /locus_tag="ACGI6B_000142"
     CDS             110895..111035
                     /locus_tag="ACGI6B_000142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749617.1"
                     /translation="MNKYIILEKYNYYYDDKCMTLYHLENIYTGLRFSIEKIELEYYN
                     IS"
     gene            111059..111232
                     /locus_tag="ACGI6B_000143"
     CDS             111059..111232
                     /locus_tag="ACGI6B_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749618.1"
                     /translation="MYRYDDYVNLINDLELPKEIRNKCVRVIMTLENMQDIMVVHKFK
                     TYVVNEKYIEKVA"
     gene            111352..112101
                     /locus_tag="ACGI6B_000144"
     CDS             111352..112101
                     /locus_tag="ACGI6B_000144"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HFG9749619.1"
                     /translation="MFKEIFEKVFMSEEVSKTVKEVNNIIKDTKIQVKGGFGELRLDF
                     ILSRLGKDYIIIKDIIVPGSNKTTQIDSIVISMYGIFVIECKNFSGYIYGNDKDKVWT
                     QIVGKTKNTFYNPVRQNYAHIKAIENIIGNKYNIYSIIVFSDKATLKNVMISGNNIIV
                     INESEILSTISKCKDVTIDKEEIKIIRDKIFDCMKTTNQNTREHVRNLKNITEEDKCP
                     RCQIGHLIKRKGQYGEFWGCSQYPKCKYTRK"
     gene            112390..113502
                     /locus_tag="ACGI6B_000145"
     CDS             112390..113502
                     /locus_tag="ACGI6B_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9749620.1"
                     /translation="MNAFIRKRNKNYVVYLEFRDDESGKRKQKNMGAFDKKRDANKRL
                     AEVKDSIYKDSFLVPNEITLAGFILDFLEKYKDNISASTYKSYIAICKNHINPSIGKY
                     RLQELRNIHIQNYIDDLAGNLNPQTIKVHINVLRLAIKRAYRIKLIKENIIDGIESPR
                     IKKFKNEIYDKEQMLKLLELAKGTNMELPISLAIGLGLRLSEVLGLTWDNIDFDENTI
                     TVNKITSRLDGSVILKEPKTESSVRKIFAPIELMNLLKNYRLEQNKKLLRSIVRNEYN
                     LLFFDRKGNPIAEDVMSKKFRKFLENNDLPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNINTTLNVYSHVLKEMDKEASDRISENLFKAN"
     gene            113739..113870
                     /locus_tag="ACGI6B_000146"
     CDS             113739..113870
                     /locus_tag="ACGI6B_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749621.1"
                     /translation="MENLMMSIIAGVIASYIYDKLKSHSNATKSGWELNIKFQRKSK"
     gene            complement(114000..114200)
                     /locus_tag="ACGI6B_000147"
     CDS             complement(114000..114200)
                     /locus_tag="ACGI6B_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749622.1"
                     /translation="MKLPELEINISISIPIFLVISYLAIVSIVMLGISALNNFTNITS
                     VPINALGSTVGFVIFQLLVSFF"
BASE COUNT        43117 a        14491 c        15570 g        41761 t
ORIGIN      
        1 taaccaaaat tccattcagt ctgcattttc tcactctcct ctctaacaat aagttattaa
       61 ctcatttaca ctagttgcaa tattagataa accaccattt accttttctt ctagattaac
      121 aaatctatct tcgattttct tagacgaata agtagtcatt tcagatactc tgttatcatc
      181 tacagttgca ttaataaaat gagtttctgc atttccattt atcacataaa cgtttaattc
      241 tgaccttgtt tcacttctaa tttctataga attatcatct ataattttaa aatttgtaac
      301 tacattttct tttgtagtag catctataat atttacaact attctctgtg ttaacaaacc
      361 atgtgttaca gttgctttaa atccattttc tgtatcatcc acccaatcat caattgttat
      421 tgtttgagta gatgccacat ttgaaccacc tgcgataagt tggtcaattt tagtattcaa
      481 ctctgtttta gcagtttcta tatttgtatt aactttttct atttctgtat tagtgttatt
      541 aggtacattt tttaattttt ttactacatc ttcatttaac aatttatcac cttctttttt
      601 atttagagag ctacattagt aactctctat tttgaacata aaaaaaagaa ctcttacagt
      661 tctatgataa tgaaatctct agtgcttcac acatttttct tacttcttct cgatatttaa
      721 caggtacttg gtcaattgtt tttaaacctc tctctatcaa tcctacataa attgctgcca
      781 tactacttcc tccttcattt aattttatat tttttaatga catagtagat gtctgttgac
      841 tacttaatac catttcgcat atttcagcag tcgctaacat taaatctgtt atgtcattat
      901 cttgcgtttt ttgacttgaa tctaaatcat ctaattcttt tctttgtctt gacttctcaa
      961 ttttttcttt atctattttt atcatttaat cacctcttaa agtgtttcaa catctaaatt
     1021 gaataacata gatggttttt ttatagttgt attagtgttt ccttcttcta ctattacttt
     1081 aggttgtgca gattgtatat tcatatcttt taactcatat atctccttat atccattctc
     1141 agtttttctt gtctctactt taaccatatc tttaaatgta tctgattcat ttatctttaa
     1201 ttgtactttt aatttattta ctaaatgata aattggattg ttagaattta aatcttctaa
     1261 cagaccttta ttgagtgtaa gtactacatc ttgtgaatta tcaactatac tagatacatt
     1321 ataagtattc ccatcgatta atacagttga atcttttact atagttgctc ctgtttcttt
     1381 tttataaata taaacattta tatataaatt atttccaatg tcagaatgtg ctttaaatac
     1441 aatcttattt tctccaagaa ctaaattctc tggattaatc tcaaacgtga ctgttcctgt
     1501 agataagtct tctattatac ttgaactaat attatttata aacatctcta tttttgtcaa
     1561 ttcattattt ggattttgca aatctatttc aatagatgag tatgacttat tctttatttc
     1621 caaacaagtt ttctctgaat ctacttttga gattatatat aatgtttcat cttttattaa
     1681 agcatccact atatcattaa catctcttgg aatatatatt tgtgagaaag tatataacat
     1741 agaaccaatc tctacatctt taattttata tgtttcctta tttatccctg taacatacaa
     1801 agtcttatca ttttttataa caaatatatg tttatcatca taagatttta tatattttac
     1861 attttctaat actttagtaa ataatttaat aattgtattt tttgtattct cattcagtcc
     1921 taaaatccca tttatattat atccttgtac gtataattga ccttctgtag ttatagccaa
     1981 tatataatta ctataagcat atatctccct tatattagaa atagttaata cttcttgtaa
     2041 ctcattaacc tcatcattat gccctattcc aagttgatat tctgagtttt taccaataga
     2101 aaacaatgag ccatcatttt ttaagaagta agtaaaatta tcaccacaag caatatctat
     2161 tatatttgtt aagttaattt ttataaattc agaagttgtt gttagctcag atttatttat
     2221 agcaagttcc ccaaattgat ttgaaccaca accatagaca tccccatttt ctaatatcaa
     2281 aattgaatga gttgatgata aagacatctt ttttatatta tcaaaaggca atgaagttga
     2341 gtaatttcca gcatttgtgc caaatggtat tctataatta atactatcat cattatgtaa
     2401 aaatatcttc ttatttacta atagtccata attatttctt ataatagaca tttcattaga
     2461 aaaacttaaa tcactaggat tatcaccaga agtgtttgca atctgatttt cttcatgtcc
     2521 attaaaaata cctacttgac caactacagc attacctttt ccttctaatt tattattagt
     2581 atattgaact ataagaccat tatgctgtgt atatacctgt tttacattaa ggcaatttct
     2641 aacaggtgtt ttttgtacaa catcagaata attattgatt tgacccaatt gtctactacc
     2701 aaacacatat aaggcattat cattatctat ataataagca tttttaccat ttgcatatat
     2761 gtgctttata ttacttgtta atttttttaa tactacagta ccagattcat taaaacctat
     2821 accatcatca gcatttcctc cagaatataa atctccattt tcaagtaaaa caaaaatagt
     2881 gtattctcct agagcaacct cttttatatt actattaaac gctaaatcct tatatccttc
     2941 aatatatcct gatgaattat tatatgaagt accaacacct aaacctccta atgagttata
     3001 tcctgtgaca aatattctat ttttcattgt aattatagtt ttgccttctt ccaacattaa
     3061 atttgatata gttgatgggt ctgttaatcc ttcaatattt attttagtta aactgtttga
     3121 atttgtattt gaacttatgt aatttctatt tccacaaccc attaaagaaa ttatattatc
     3181 attctccaat aatataataa aaacatacct tccacaaaat atatccttta ttgttccaga
     3241 aatatttact ttagtaaaag tatttctatt aatattatcc cctaatccta attgaccata
     3301 gccatttgaa ccagttgaaa ataattcatt gtcataggtt agaatgtaag aagatttgta
     3361 acatgttatt acttttttta cattatcaat aggcacctta gtaaaagtac ccctatcaat
     3421 attatctcct aaacctaatt gccctaagtc atttgttcca attgaataga cttgattatt
     3481 atttgtaaga ataaataaac aatcattcga tggaataaat tcttttatat tatctatagg
     3541 aacttttata aattcagacc gagctttaat tgtccctaac ttgttaccag cattattacc
     3601 acagacatat ccttcgctat tatttaataa tatatataaa gcttgtctat cacttttaat
     3661 ttcttttata tttgatatga tatatttttt agaaaattgt tttgatatat ttacaaaacc
     3721 atcatgtcta acatcataag gtttttttat gccaaaacag ccatgagaat ttactccaga
     3781 acaatatatg ttcccaaaat aatctttaat tactatcctt aatctactag catatattac
     3841 ttgtgaatta tatgcaaaaa aagcatttga taatgtgtat ttttgactat tagaataata
     3901 actactgtct cctctttgcc cacctgcatt tctacctctt atttcaaatt tagactccat
     3961 ccttgtttta cttgttgtat ataaaagatt gtctgttcca agataatgat tataataagg
     4021 catagaagac gatataagac ttcttaaacc taaattcttg tcttgggatg aagctccaaa
     4081 agtcgaaggt cttcctgtga attttatttc tttatatcct ccatcactag catctaataa
     4141 taaacataaa ttatagttag aaaccagaac ttttgataat ttaggttcat ctattaaaac
     4201 tgcgacttca tttgcatttg agtttttact tcctacaaac tctccaaacc ttgaataata
     4261 atcatcagtt acagaatttt taatacttgt gctataataa ttatgtctcc cagaaacata
     4321 gtagttatta gacgtagttc taattaaaca tccattatta aaaggaataa tttcagcaat
     4381 gttagataca tctgatactg ttgtatctgt tttatatctt aaatatttca tcttaacaaa
     4441 aacgctccta cttgaggtat cattaagact taattcgcca aaaacattat atcctgctgc
     4501 ataagatatt ctgctattgt ctatgcaata tgaagaattt gaatatgcaa taatttcatt
     4561 tacataagtt acagtactta ctttagtaaa aatatttcta ttagtattgt cattaagtcc
     4621 taactgccca taattgttta aaccagttga aaataattca ttagtagttg tttttaataa
     4681 cgcatggtta tctcctacag aaattttgtc aacagaagaa atatttactt tagtaaaagt
     4741 atttctatta atattatccc ctaatcctaa cactccaaat acattatatc ctgttgaata
     4801 taatgtatta tcacttagta atataaacgt gaaatatgag cctagattta aatatatatc
     4861 ttttatagta actccttctt ctaattccaa tggcactcta gtaaactcat aacaatgacg
     4921 attatgacct aaacctaaac ctccataagc gttgtatcct gctgcgtata gttcattacc
     4981 attcacaaca ataaatgtag aggtgtcatt tgctttcact aaagagatat tcacaaaatt
     5041 attttcaagc cctatatcta aaactctttc atcattttta tttattaata atctattatc
     5101 aatagactca atatgattac attcttcaat atcaaagtcc tcaaattttt caaatccata
     5161 tataaattta tcatatcttt ttaaataaga aaaactataa ttgttatctc ctgtcatata
     5221 caattcattg ttgctagtta atataaagct tatagcatga gcacatttaa ttttacttat
     5281 atttttaata ctaatagaag attttctagg agtatatgtt gatctatcac aatttatttt
     5341 atctcctaaa cgcccatctg ggttggctcc agttacatat atatcattat tattagctat
     5401 ataaaattta tgatatctac cactttctat attttttaca ttagatatag atagttttgt
     5461 taagactgtt actgtagctc ctccaccaac accaacttgc ccacttgaat tatctccaca
     5521 accatagaca tccccatttt ctaaaagaaa atttacagca ccttgagcag tagaaatctt
     5581 tattacatta tctaaattta ctttagtaaa aatatttcta ttagtagtgt cattaagtcc
     5641 taactgccca taattgttta aaccagttga aaataattca ttatttaaag ttaatatata
     5701 tgaacaactg actcctgcaa atatttcttt aacactcaaa ttatcaattt tagtaaatgt
     5761 atttacagta gatgtatttc ctaagcctat ccctccaaat ttattattac cacatacata
     5821 aagctcgttg tcatttgtaa ctaacataga atagccattc ccacaagcaa tttgttttac
     5881 attacttata tttatcttag taaaagtatt tctatcaata ttatctccca aacctaactc
     5941 cccatctgtg tttaagccag tagaatacac atctccgtta tttttaagta aaagagaatg
     6001 agttccagaa gatgaaacat acttaacatt gtcaatatct atttttgaaa aatatcttat
     6061 aggtgatact ttatttccaa gtcctaaatt gccaaaaaca ttatatcctg ctgcatataa
     6121 ctttaaatca tccttaatta taaacacaga accagaatcc atatctgtaa atatatcttt
     6181 aacattaaaa gtatcaaaat ttttattaaa ctcttctatt ttaaattctt tgtttccaaa
     6241 taaagaacct ctttgtaata gtttattctt tttcatataa ttccccctat ctcacttcgt
     6301 aggaatactt gtttattttt atttcagtat cacctttttg ataagattct tctgttttag
     6361 tttcactata gttgcttcct aaatcatgaa tatatcttat tttgttgcat aaaaaaggaa
     6421 ctgttacatt tttaaataat acagttccac tatttgaaag taactctaat tgttgcttaa
     6481 atttattaag ctttaaacca tctaaattac ttatgttttt agaatcatat agggtatcat
     6541 accaataccc agcttcatct gctgtagctt ccttatctaa ttctaattca aatagaattt
     6601 taagtatttc cacagacatt ttattataat tttcttttaa tattttcaat tgctcgtttg
     6661 tatttccacc agattcaaga actttaacac tagcatctaa ttcatttacc gcatctgata
     6721 tatttttaga tactgtactt aattcactcg aaccaacttt actttgtatt agatttattt
     6781 tctctaagtt agtagctata ttacttttat tttcatttat cttagttaga ctctcatcaa
     6841 attttgcatc aattttaata ctagagtaag tactatctag tgttgttata ttatcattta
     6901 ttgtagcttg tataacttct ttatttgtat taccatttac aacagttact tttacatcta
     6961 tgttactttc attaaatatt tctatcgtat tagaatctac tgtagtataa gaattagtca
     7021 tacttttctt agttgcttca tctaatattg ctacaaatat attttctgta actaaattat
     7081 gattaactat aagtttaaaa tattctccat cagcaaccca atccgattca acataagatt
     7141 tactaaaagc tacattagtt gcagcagaag tcatttctgc tattttctcg ttaactttct
     7201 caaagtcata tgttaatcta tcctttaaag tatcctttac agttccatca acactttctc
     7261 tagcttgtat taattcgcct ctagcatcaa gactttctaa ttcttcaaac ctattttcaa
     7321 attcagttat tttattacta acagttgttg tcatatcagt tttagcagta tttacttcaa
     7381 ctattttgtc atctacttct tttatctttg tatctaattt tgtagtactt tcatcaatac
     7441 aatcttctac attagatata gcttgattta cttcatcaag tttgtaatca atttgctctt
     7501 ggttatctgt aagtttatct tcaaatgtct tatcatacaa atcaaaagta ttattagctc
     7561 tagtgtctaa gtcttgaaac ttcttgtctg tctgaacttg aacatcaagc attttatcat
     7621 cgactatatt atctatttct ttagacttag catctatcat ttcatctaat tggagttggc
     7681 aatctgtaag tttatcatca acttgaattt gcatattctg aatggattct tcacgatttg
     7741 cctcttgatt ttgacgtgtt aactcattaa actctctttc ttcttcttgt atgtttctta
     7801 tatcttcatt ataaatccta atctgctctg ctttatccct aacttcttca tttccaagta
     7861 tagttatttc aagctgttca actcttttag tcaactcttt attatttttt ataatctctt
     7921 ctgcttgggc tattattacc aataatactc catattcatc agttgaggtg atatcatcat
     7981 cattaatcat accctcttca actctataat aaagatttgt agtattgagt aaagttccat
     8041 ctttcacaag ggatagttga aatgtattta taccaacaga agcaagtgct tgtcggctaa
     8101 gttctatctc tacaatacca ttttctgcat ctacaacttc acattcacca ttaacctttt
     8161 gtccatcact ttttgtaatg ctaactaaaa ctctatcata ttcagataaa tctattattt
     8221 catctttatt tttcaatata gcttcaatat aagctgtttt aacatcattt tcattataaa
     8281 taaaataatc tagcaatctt ttgtttattc tataattact aaagtcgacc tctatggtat
     8341 gattctttat ttcataattt ttcaaagtat cacatcctat tgttctttat tttgttcttc
     8401 tagttctttt ttcaagttct caatttcttc ttgttgttgc atgttgagtg ctttataaag
     8461 tattagttca ttatataaac tttttatctc ttcaaatgct actgtatatg ctttttgtaa
     8521 atttacttcc atattaatat cctcctaaat tattaatttt ctcgtttaat tcattaatta
     8581 tcatctctaa ttcttctctt ttttgtattt cttcttgaag tgcagttgct acaacagttg
     8641 tataaccaga tgggctaaac attaatccat tttcttctcc atagtcatat agaaatgttt
     8701 ttccaacata actatcttta aaatcattag ctataaaccc tatttgacta tctgcaattg
     8761 tcctatcttt ttctgctata taatcaaatg ttgctggttt aaaatcatct cttataaaat
     8821 ctaaaaatgg tgttggagtg atacttgaac gagttctatt cttaatatct tttatgtata
     8881 ctatattctc tttaaacttt ttatcagatt cactcagagt agaacgagca tataaatatc
     8941 tccatttata acgctcacaa cctaaatcag ttacagaacg ctctggataa aatgaatatc
     9001 ctacacctaa actatctcca tttgtaaatg taaaagtagc tccaccacat cttacaaaag
     9061 tatcatctct ttcaacaata aagtgttctt ctccatcaac ataaatctca gcagtatcat
     9121 atcctaccca aaaataatta ttgctatcat attttaatct tattgctgag gcttgactag
     9181 aaccatctga ttgtcttgca tctattgcaa atccagacct gctacttcca aataatctga
     9241 ttattggttc atgtccagat ggagtagtta atatatcaca tgctatagct ccttgattta
     9301 ctgttagacc actcctgctt atctctacat aatttgatat atcattaaat ccaaattcaa
     9361 ttttatcagg tttaattcct acaattgagc cagagttatt cctcgtaacc atcatggtta
     9421 tatcatcttt agttaattta atttcagcag catttgtttt agttgcacca tcaacttttg
     9481 ctacttctaa ttttatttct ttagctgtag catttatctg tgaaaattcc tctgatatac
     9541 ttccacccat acctgttatg taaattttat cgactgtatt tttaagttca tttatcttta
     9601 agttatattg agcatttgca tttatagcta aaattctgtc tgaaggtgta attatactat
     9661 ctgaaataac tgaattaatt atagagttta gattggtact tgctgcatcc aatgctgttt
     9721 ttgaagtgtt tattgctact aagtctactc cagtagttct atcaataagt ttttgtagct
     9781 ctgtatataa acttgctttg tctgtatcta ataaattaag ttcaatatca atcttttctt
     9841 tttcttcatc tgtaacttca ttatcattaa aataattaga catattagaa ctaaatgtac
     9901 ctaaatctgc atttaactta attatagctt tatttatgtt ttctcttaat gttatatttt
     9961 cttgtgaacc accaaaattt ttagtaactt cttttactaa tatatttaca ttttcagctc
    10021 tttggtctat aagagacata ttttctttta tatgagatgc atcttcttgt acttctaaaa
    10081 ctgtttgatg tattccatct atatcttttt caataactac aaacttttca ttatgctttt
    10141 catcagtctc atataatccc caaatctgtt cttctgtatt ttttcttgaa ttaccatcaa
    10201 atgaaatatc aaaaatagaa gaatttatag gaacttctgt gtgttttcca ttgtcattta
    10261 caactactaa aaattgtgtg tttccctctg gtaatcttac attagaaact tttaatgttc
    10321 ctggattatc agcatcccag tatatatatt ttttatttgt ctctgtatct actacttgat
    10381 aaagtgtatt gttgtaggta acaattactc cagtaattct aatccatgaa tctgctggtg
    10441 aattatctaa tatcaattta catcagctcc ttttgtatat ttttcttagg aatatattca
    10501 tctggtagat taattctttt cttctgttgc tgaataaata aatgtctctt agagtttaat
    10561 gttctcgttg cgtgttcagc tttagtcaaa tagtcagcta tagtcctttt gtcatcttct
    10621 tttacttttt tattacttaa agttaattct aaagtatcca atttcccatt tttaaaattt
    10681 tgagtaaaag atgtaaaata gactaattct tcttctttag attctttgct atgtaacact
    10741 ataatatctc ccagacttaa atcacccttc caatgttgtc taaaattaat atctattatt
    10801 ctatctaaga agtttataac atccagagtc cactctttgg ttggaataca ttttaaactt
    10861 aattgtctct taccttctgc tattaaatct tcaactttta aaaaagcatc atttgtataa
    10921 gtgtcataat agagaaactc atttaactca tccaatagag cctcattaaa tattaaatat
    10981 ccatcctcat cagtcgaggt ttctctctta caaagaatat ttatatcttt tatactttca
    11041 tctaactttg ctatctcttc ttctaaatgt tttatttgaa catctaatat gaccttttta
    11101 tcttctagtt cacttatttc tactgctaac ttagctgaat ttacttcatc tttatgttct
    11161 ggattatcat aagttttctt tatatccttt ttagccttta tagtctctat tacatgtaac
    11221 cattcattac ttttactatc acgttctctc tgtttcttaa gttttgtatc tattagttct
    11281 ctccaaattg gttctcttat ttcattcatt tcctggtatt tctttatagc tctactaagt
    11341 tcttcactca tttctttatt gtctaagaaa taagaataat tctcaatgta atcatatcct
    11401 gtaactgtag ctcctattat atccatatct tcactacctt caagcttcag tctagttact
    11461 atatcatcac tatttgtagt tctttcaagt gattttatgt agttatcatg agataaatat
    11521 aactgaatgt tatctccaaa actgtctata tgatacaaat taactaaatt attataagtg
    11581 tcaaaatcag ctatacactc aaattgttct tttagttcat tattgaaata atctaaccaa
    11641 ttagaattaa tactctcttg ccatcttact ttttctctct tattaccttc actatcagtt
    11701 tcataagcaa ttgaatcatc cacataacca agtttccagc ctgtctcttg ttttaaataa
    11761 tcattaagag atataataga tgcttcttca tctttggtaa acatctgtaa tccatagtcc
    11821 tcaatattca catcaatttt tcctagttta acttctttag atttagctgt tactactttt
    11881 aatttatcgt ctgtagtcac aacattctta actacaaaat attctttatt attaagacaa
    11941 ataagacgtt cttcttttac ttcttcaaaa atgggattta ttattttatt aaataaaaat
    12001 ctatctgtaa tatattttgg aatggtcagt tgtatttcat ctactccacc aagctcacgc
    12061 tttatagaat ttaaaaagtt gattgggatt tgacctaaaa atgatttgtt catcttgtga
    12121 atggttaatg tgtaatcaga ttttatctgt ttaagattca cattaaaatg catattttat
    12181 caccttcttt gtattagaaa agcccatcaa ttaagatgag ctaaaatatt ttattaatct
    12241 aaatattgta acaagtttaa tagcttgtta catatatgat aaagcaaaaa accactcaaa
    12301 cgccaataag agtagtttca aaagataaat tgatttttaa ttaactttaa ggaaacaatc
    12361 actctttgta ttaagtagat taaattcctt acttttatta taccacagat acgttttttg
    12421 taaaacagat tcgtttacag ttaataaata aaaaatcact agataaagac gattactgat
    12481 atcaactaat acatatttaa aataggtata ttataaatca tttcattatt caaaattcac
    12541 ttcaatatct tcaaggtctt tttcattaat atttaggtct ttcagaatat ccctttgaga
    12601 tattagttct ttttcatcta tatctttcat tcttatttct gcctcaataa gaagcttaca
    12661 atcttctata ttatttatca tttgtaacac ccttaaatat ataccagaat agaaaaatag
    12721 ccacttttta aggtggcata gataagtagt aattcttatg taaaattttt aaattttctg
    12781 ttatctgtat tatatcttta ttctactcct gtcgtcaata taatattaaa tttaacatat
    12841 tggaaaatta tggtataata tttttagaga ctacagttta gactattagg gtgatggctc
    12901 tttagttaaa atccccagta agaaaggggg tgagactata gagtacttag ttttgttttt
    12961 aataatgata attatgatta caagaataat caatcatttg acaaagctaa tagatgcaat
    13021 cttaaacttg aaagcatcta ttaggaaact aaagaatcac cagactggcg ggtctagtga
    13081 ttctgaaagt aatacataaa tctattcttt ttattgttaa ctttaagaac tacaccctac
    13141 agcaaatagt ttgtagtctc ttttttatgt ccctatgata taaatttata tcatttatat
    13201 tactattata ctccaatatt atcaaaatag tcaatgttgt cattatcttc attgaaaaat
    13261 aaaaaagcta tattattaat agaataactt cttagaacac aatattcttt tacaattgta
    13321 gacaatataa tattaaattt aacatattgg taatttatgg tataatattt ttagagacta
    13381 cataaaaaaa actattaggg tgaatcttca tatacataag ccttcccatg tcaaatcaag
    13441 aggggaggtg atatgatatt atgaaatatc taatttgttt gttaacgata ataacatttc
    13501 ttacaactat aattattagt ttgattaagt tggttcagaa tttaaataga ctctttaaag
    13561 aaatagatga gtttaagaaa acgttgcaaa aaattaagaa tcacccgaac ggcaattcag
    13621 gtgattcttc ctctaattaa cacttcgctt atttatgaag agacacccta gcacaaatag
    13681 ttgtagtctc tttttttatg tctttatgat ataaatttat atcatttata ttactattat
    13741 accatataca tattattttt tgaaataaaa tctggatttt attagagaaa tctggatttc
    13801 tacttaaaat cctttgtcta tataaataat ctatgattta atattttcta ctatttttct
    13861 atatactcta ttctctaaat ctctaccaaa tttttccaag tctttcatag tatccttagt
    13921 tacatttcct tgaacaacaa agaatggtga attaaaatta actgaagatg ttggaaatgc
    13981 acttcttgaa cgagaatttg aaggaggatt ataattaggc atagcaaact tacttaaatc
    14041 taattcatct aatatgtcat taatatcttc taatgtctct ttaccttctt ttagtgtatc
    14101 aagccattct tgcttagtaa cagaacccat tgccaacatg ccatcttcat atttgtttat
    14161 gtattcaatg atagcatctt gaagtttctc aacttctcca tcaagaccta caaatttacc
    14221 agaactgatt gcttcattaa ttaaatcaac taaattttca tctgagtatt ttttctctaa
    14281 ctcttctttt aatttatctg cttcttcttg tagtctgtca gactccttat catacatatc
    14341 attgacttgg tcgtctacat ggtcttgtac taaatcctgt aatttcttct gttcttcttt
    14401 taattgtttt tgtaaatctt taagtttctt ctgaccactt aaagaactat ctctttcagc
    14461 tattgctatt tgtttttcta aatcacttat tacatcttgt tgttcttcat aatcatcttt
    14521 atatttggct tcttttcttg aatcattgta agcatcttgt tgctttttaa gagaatcaat
    14581 tttagcttta gtttccttgt ctatttcttt aagtctctct tcaagttctt ttttatacat
    14641 gtctctaatt ttctcttgta gactttgagc ttctttgagt tggtctttat aagcatcttg
    14701 aatttcttta tttaatgctt caatttcttt cttagattca gcaatttcct tattagcatc
    14761 aggtatttta tttattaata aatccaaata tccctctgct gcatcttcca ttcttttaaa
    14821 ttcttcatca gtcatgttct ttttaagttg atttttatgt tgaatgaagt tagttatatc
    14881 gccattatct ctaaatgtaa atccatagcc ttgtagcttt tgttgtagct ctttttgtgt
    14941 ttcttgaaga tatgctgttg tttcagatag ttctttagcc ttcttggcca ttaactcttt
    15001 tttcttttct aacaactcta tctgctcatc acctgtagca tttttaagta atacatctat
    15061 cattgctatt tcattattta cttgctctac atctttgtac atagaagtcc atgtggaatt
    15121 cttattaagt ttccacattt cttcttgtgc tttccttaat tcctcattgt gttctttaat
    15181 tttattattg tattcttccc attcttgctc acatttaggc agttctgtaa atgtaacttt
    15241 aatatattct tcaaggactt tcttagtttc ttccaactct ttttttctgg attcatcttt
    15301 tttattttct ttgtcaccag actttgaatt taatttgtct aattcttttt ctattgatat
    15361 taacttttct tcatagttag ttagatttcc agtatcatca aacttaaagc ccattttctt
    15421 ttcttttaat ctatttttaa gtctgttact ttcagcttta tatccatctt gttgttgttt
    15481 tattaaatct ttttgttgag caaataattt attttgctcc tgtaagtatt taatcttctc
    15541 atcacctgta gcatgttcca tttttttgtc taatagagct atttcatttg ttactgctga
    15601 tatagcattt tctaactctt taaataattc tatgctatat tttacagatg agtctattgt
    15661 gtcattgttt attgtaactt taggtttgat tactttaaaa gctgatgtgg ctcttgatac
    15721 tgctcttgag aataaggatg gttttgttac aggagtttcg tcacttattg gtgctggttc
    15781 tgatgttcta ggttgggata gattagctgg aattggagtt ccattagtaa cagaggtacg
    15841 ttcagtttta ttggtcttat ctgaactatt tcctccacct ccaccaccag aactattggt
    15901 tactttatta gttacaaatg tagtagtgaa tgtttttcct gcatagtttg atttaaatga
    15961 ctctattttc ctaataagcc ctgatatgtt ctttgcagca gttgcagtta atgcactaaa
    16021 tgtagtcttt atagtttttc caccatatcg gctaacataa ctttgtactt tggctaacaa
    16081 acctgataca ttttttgctg cttgtgcagt ttcagttcta aatacaagtg tagatacttt
    16141 caaagctaag aattgtttta ctctagctat aagtcctgaa atattcttag ccccttgttc
    16201 agttgaagta ctaacattta tatgataatt accttgccca atttcttgtg ctttttgtga
    16261 taattgattt aactgttcag atgcttgttc tgcacctgta gcttcaatag taggagtggc
    16321 tttttcatta ttaagttcat ctaacttttg ttttgtattg tcaatttcat ctttaccaga
    16381 gttgtttatg gataactcaa taaccttagt tggatttgta ttttctataa aattggttgc
    16441 ctgttcaact tcagtttctc cttctgtctt tatttgtaca gttggagtta ctgttttatt
    16501 atctaactca tccatttttt gtttagtact gttaagtcct tcttcattgg taacttgagg
    16561 acttacaatt ggaatataag attcatttcc atattcatct aatattctca tgcactgaac
    16621 aactttttca ctattattaa cttgaggatt taaattagga gtgtatgttt tattcccatt
    16681 cttatctaat gtttctatgc attcaactac ttgtgattct ccatttactt ctgcatttac
    16741 taaaaggtca gctttagttc catctgcatt ttgaactgta ttaacagttt tctctgcatt
    16801 ttctcctcct tcaacttcta ttccaagttg tttttttatt tctggaggta aattgtttat
    16861 aaattcttgt acatttttag ctttttcaag ttggtctaaa ttgtttacta taaaatcaat
    16921 aacagcttcc tttttatcat ttggaacatt attaagaatt tcatctatct catttagttt
    16981 atctggattt ttattgatta tttcaaatat agtttctttt ttatttggag ctttatctat
    17041 taagttttgt aatgtgtcta atttatctat acctattact tctgtttcta tctttttttt
    17101 gagattatca ggtaaaatat catagacttt ttttgcttca gtcaatcctt ttttatctac
    17161 atctatatca tattttagac tcattggagc agatattttt tttctagctt cctctaaaga
    17221 atttacatct ttaaaagcat ctgcacaatc tgtagtaaaa gacaactttg tatcttcatt
    17281 tttaaatcta tccaactgtt cttttaatac actcaagtct ttttttgcga aatctagtcc
    17341 tttattatta atttcagtca caattttatc tgatttaaaa gtgtcttttt tgaatttttc
    17401 taattctttt aattcttttt tagctttttc tccattgaca ctaggagtga tttcaggggc
    17461 attgtctcct gtaaaagcat ttgcaaattg ctcatttaat gcttgaacca tatttcctgg
    17521 taatgcaggt aacttgtcac tgatttttag tccattttta agaatgtcat ctgttaattg
    17581 taatttaccc ttaaataagt catttaattg tttaacaaca tcattagata attgaccttc
    17641 gttttgcaac gtagttgtta cttgtagggc taattgagcc tgagcttcta tttgtgaatc
    17701 ttctccaaat gtgaatccat ttattatatc ttttagttgt tgtggtaaat cttgaccagc
    17761 ttctccactt tgtatatctt caagcagttt tatatttact ttaccattaa cttctaaatc
    17821 ttgagaaaaa tcatttaatg catcaaattg gtttttaagc tttattgcaa aactatcttt
    17881 gttttcaagt tcagctaaag atgaaccata acttttcaag aaagtatgta atttcatact
    17941 ggctaagtct aatccagaaa aaccttgttg taatccaata agccaagtat cagcacttat
    18001 acttccacca cttagctttt gaagttcatt cgccaaccct tgtaattctt tggtatattg
    18061 attcatatca ccggatgcct gataatttat attagcatca gttatcttct tattccactg
    18121 gtctaatttt tcaacaggaa ttgactcact tattttatca aaagcactta taatgctatt
    18181 ctgttgtcct acactatagt catgccaatt catattagct acaagctcat tcattttact
    18241 tttcttttgt tcagattgtt tagcatatgt tttagatata tcagaattag ttaatctatt
    18301 aatcgccctt ttctgcatta cctcttcttc ttcattatat ttttcatatt cagtaagaag
    18361 tttttgatga tgttctgcaa aatctgtttc ttgttgattg taagcctttt ttaatgcttc
    18421 tgtttgttct ttaaaattag atttacttaa agcattttta aataaattat gctcatcttc
    18481 aagaacttta tcaagttttt tcttttcttt atcaaggtct ttttttgtat ctggtgcagt
    18541 ctttgatgta gttattgcaa gttcatcttc ttgagacttt aaaagtttat ttttttcttc
    18601 tatagccttt cttaatttat taatgacctc atctaagtta ccactcatat ttagaatagg
    18661 gtcattattc tcatctacac ccaatactaa ttctggtgct atatctgcaa tttgttgttt
    18721 aagttcgcta aatctttcag tttcatcagc agtcttttcg gtcttactag ctaaattatt
    18781 gtactcttct gatatacctt ctaaactctt tttcttttgt tgcaaggagc ttatctcgct
    18841 tgtagtagtt ccaataactt ctttttgttt ttctgctaat ttttcatact tgtgaatagt
    18901 atcatctatc cattgtatac ctttagttat agcaaaagta gccaaaccta tcatagcccc
    18961 gtttaacaaa cttgtcgcta tatttaatgt tccaatacca atagaagtcc tagataaact
    19021 ttgtcttaat gctcccaaac ctgttcctgc tctagtaatt acatttgctc cattagcacc
    19081 agctacattt gctgccatta ctgcatgtct aaatgattgt agtattgtag ttgtagcttt
    19141 aataggagtt cctatcatat ttaaaggatt tataagtcta gccacatttc tagtaccatt
    19201 tgtcattccc ataccagaga ataacgtttt taaagtcata aaacttccta ttaatgctgg
    19261 tgttgttaat tttaatcttt ctaatgtttt tataacacta tttattccat tagatacaga
    19321 tattaaacca tctaaaaatc catatgttgt atttgaatta aatacagttt ggaatatatc
    19381 tgcccatatt gttttcaact catttaaacg ccctgcaaga ctatctaaat attgtttgtt
    19441 ttcagcctca gccgaaccaa attgtaaccc ttgtgaaaat tcacttctta tttctttaaa
    19501 agtctcataa ttacccatta aggattggaa tatagctgct tgttgcttac ctgctattgc
    19561 ttcagctaat cctttttgtt catcctctct gagttctccc catttgcctt tcaactcatc
    19621 taagagttga accatattct tcaattcacc agtatcttta tttttataaa tagttattcc
    19681 agctatttcc tttaaacctt ttgcggtttt atttaaagaa agtgtaccat catcagctga
    19741 aactttcata cctgctaaat ttattgcgac actttttaaa ccattaccta ctttttctgg
    19801 attttgtatc gcttcatttg ttgctgttat catacccact gtgtcaccta aactgacacc
    19861 atactcatga agtacactac ctccacgttt ccgtttatct tcaattaagt tcgctacact
    19921 taatccgttc tcttatgaac tgctatatgt ttccatacag acgagactat atcttcatcc
    19981 tataaggatg ctccccattt ccatatccaa tagcttgata tgtacggttt ataacctagt
    20041 cgttgaacct tactctattg agtcttggct gctgattgtc cctatctttt agattattac
    20101 acttagtact aaaagcctaa caggagtttc cagcagttaa aggagtttat cattatatat
    20161 tactatataa ggcgactaaa attaatcgct tcggcaacac ctgctgttcc aattgcataa
    20221 ttattccctg catagtttca aattgttatc ctacaagctt tttatcttgt agttcttaca
    20281 tttcttattc atgtaagttc agcatatatt ttcatcttca agattacttg ttaagatgga
    20341 ggacactcgt gggaaaatta tattctatac tattttaaac ataaaaatag cataggttca
    20401 tttcctatgc gttacggtga ttaaagtctt ttaattcttt aatttacctc ggtggtaaca
    20461 tctcagtctt caccgatttt gccccctcat aatattagat atttctatct aacacgccaa
    20521 acaatttagc atatccattg cagcagttaa ttctgttgtt ttctttgttg tattcccgac
    20581 ttgtacttgc atttctttaa gtggttctat tctaaatcca ttgattattg tgtttactgc
    20641 cgaagatgca ccttcaacat ccatatcacc tacattggca agtatcattg attgtcttgc
    20701 agcagcaata ctttcttgca tgcctccaat tcctgcttgt aaagaagatg ctattgattc
    20761 ttgaacttgt gatgcactca ttcctacatc tttagctgtt tgtattgctt tttctcttat
    20821 tctatctaac tcagatgtac tatcaatatc tgttggttct gcaacctttt taaggtcagt
    20881 catagagcta tcaagctcta atataacatc tttcatagac ctaattccat cagtcaaagc
    20941 atcgcccaac atatctccaa tagaataaga tgctacagat gaatagaaat cattaaaaaa
    21001 tcctccacct cttgaaactg tattatttaa tctttcagct tcttgtctag catctctcat
    21061 ttctcttgtt aatctatcaa atatattgtt aaaatcacca tttagatttc ctgccgaacc
    21121 ttgaagttgc tctaaagaac gtctaaaagc ttcaactctc tcattcccaa atgcctgtct
    21181 aatagaatct tctattctgc tgaaattaga ccttaagcta tcaactcttt ctagatttct
    21241 aacttcttga tttatctctt gtattctatt aactatatta cctacatcaa gatttaaatc
    21301 tatatttcta ctagctgtct gttttaattc ttgtaactca ttcctaagtc tatttacttt
    21361 atctgtatct atattatcgc ctaatccatt aagcctagca gatgcttgtg ttgccatatc
    21421 ttctattctt gaaagatttc tattcatatc agctaaatct cttgttgctc ttgaagcatc
    21481 aaacatatcc attctagctt gagatgatga attcatttgt gcatacactg aatctaattg
    21541 tctttttaat ctttcaattt gttctgttgt tctttcaatt gaactatctc ctagacctgt
    21601 tctaatctga ttttgaagtc tagatacaac atttgagatt tctttatatt gtcttattaa
    21661 atcttgctgt tcattattta tatttcctgt gtttccagag ccaccaccaa gtaagctctc
    21721 ttgacttctt ctacttagtt tatttatttc tttcatagta gattcaattt gctttaagtt
    21781 gctcaaagca gtattatcaa tcttaaaggc tttattgaat atccctttta attgctctgc
    21841 ttgttgtgat aacttatcta attcacctat tttcaaattt aatttaatag gtttatcttg
    21901 accactattt tttaatttct ctattttttc tctagcatct ttgtcatcaa gctcaatact
    21961 ggttttaatt ttaaactctt caaccattta ctcaactcct tttttgtata aaaaaaagac
    22021 agttcatata gaaactgtac tttttcttta taaaaaatat ttaagtttaa atatttaagt
    22081 tctgacttat tcctttcaac atataatcat aatactctat tatttcatta tatttatttt
    22141 gtaatattgt tttttggaaa tcagatactc cgtctaaaat atttgacttt tcttcttcta
    22201 atacactttt taaatcagta attattttct ttttacttat taagtcttct ttccaatcac
    22261 ttttcacaat ttcatagtta ttatattttt cagtaagtaa tcttatttca tctcctataa
    22321 tttcactttg atttttagtg tcatttaaaa attgcaattt tttattataa atatcttcag
    22381 atgtactttc ttcatctaac ttttcttctg tttctttttt tactacttca tttgtatctt
    22441 ctttactatc actacatcca ataactccaa atcctaacaa actaatcatt aaaatcaata
    22501 atattttctt aaacataata tcccccctat attctgatac attaattata tcaattctaa
    22561 aggaaaattt atactacaaa aggaaagtga cctccaaatc tttattaata agctaaactt
    22621 gtaaccacag tgtcttttgt tgtcttacat atatcttcta tagatttact ttctagctca
    22681 gttataccgt acaaatcata tacaaaagta ttgattgtat tttcgcatac atttatatcg
    22741 tatatgattc tattaatagc atctttgtac ttattaaaat attcctctaa ctcatcaata
    22801 ttcttaaaat aaagcttctt ttttttgcta agttcattgt aaaattcatc aaaatctaat
    22861 aaataaaaat tatataattt attagatgga ttattatcta tgctcaatat cttaaataca
    22921 tttttcaaaa atttattagt gtataagtat aatttaccat atttatttat tagtatttta
    22981 ctattattta taacaattga tttatcaaca ttttttaaat ttggtattgg tatatttttt
    23041 actgcatctg ctgtcaagtt ggtatttgat tctccataaa atttattata aaagtaattt
    23101 attaaactag aatttataac tcctatataa aaatatatat cttccaaatt atcttcttta
    23161 attaaattta tgatatttcc actagggaat attatagtac cttcaatatc tactgttgca
    23221 attatcttat tttgattttg accaactatt ctttgtgtta ttatcttagg tgatttgtgc
    23281 cacctcatag ttttgtcatc catgttttca atatttacaa atttatctac tttatcaata
    23341 tagtatctat gtatatcctt accttccaaa catttattta aaattccatt taattggtta
    23401 ttatctttta tatcattact acttaatatt ttcttatcat atataattcc atcacaaatt
    23461 gttttattag ggacactcat ccaaccttta tgtaaattta aataatcccc taatttatgc
    23521 ttacatttga gcatttttaa tattattggg ttgctttgta taacaatttt attatccaaa
    23581 tcatttataa tactttttat agtaatttta ttttcagagt tgtatgtatt actattgaaa
    23641 atcttaatct ttttcatatt ttctttattt atacttataa gatttatatt atttttaata
    23701 ttcggcaatt ttttattaat tataattata gcggtttcct tgtttacatc ctcaaaagga
    23761 aaaaatctat atggaaagtc taaaatagta tcaacacata catttttaag taaatatttt
    23821 cttaaatttt caaagctttt atttgtaaag tacgtattag gtgttataaa tcccaaaaat
    23881 ccatcttgct ttaatttatt ttcacaaaac tcatataaaa acaactcata caaatccatt
    23941 tcgctaatta gtaaatcttt atattttttc caataatatt ctctatgctt ttctggaatc
    24001 attttagttg aaacatatgg gggatttcca attataatat caaaccctcc tgacatcatt
    24061 acatcttcaa attcaacatt ccaatcaaaa gctttagcac aatcaacctc tttgttatca
    24121 ataatagaat ttccacattt tatattatta tctaagctag ttaataaact atacttgtta
    24181 gctgttttaa gccataaaga caatttagtt atttccacag attcttcatt caaatcaact
    24241 ccatataaat tattttttaa tatattagca tctaagtcaa aaaatgatat ctcaccatcg
    24301 gttaaatcac gaagtatttc gtttactgat tcagcttctt tttttaacat agaaaaagcc
    24361 tcattcaaaa aagctccact tccacaagct gggtctaaaa tttttatatt ttttacaacg
    24421 tctctataat ttttccaaaa gtttatatat ttttgaattt tatcagtttt atattctaca
    24481 acttgtacac gcttatgctg tatattccta ccaaccacaa ctgtttcata atcttcttct
    24541 tttagttctg gaagtttatc ttcgcctaat tcttttttct tctcatctaa atatcttctt
    24601 atggtgttat taacaatatg tttcgttata tatttgggag tataaaatat cccatctttg
    24661 tttctcttac tatctttttt cttaatatta acttgattta tttctagttt tagttcttct
    24721 aagtcagata tagatttttc aaatatatgc cctaatatat tttcatttaa atcactatta
    24781 aaatcatata aagataactt gtataaattc tcgaaacatt tatttggtat ctttagatta
    24841 ttcaaaatat aatcttctgc aaataaacct ccattaaatt gatttatgcc cttttcctga
    24901 ttacctaaat ctatagattc aaacagaccc tttacttgtt cccagactcc aaacctagat
    24961 acactttttt tccctacttg aatacatgtt tcaaagatat tctctggtat taatccttta
    25021 tgttctgcaa aacaaacaaa tataactcta tctaatattt tttgtgtctt tgttaaacaa
    25081 gtttttacat ccaaaatatc tttattattt aaaagtatat cattaaataa atttaatcta
    25141 gtatttttat ataggtcata aaagcctttt tctatggctt cctcttgctt tatattctct
    25201 tcccaaagca tctcaactct agattttcca tgtttggtca ttaaagattt agatgacaaa
    25261 agatataaaa attttttaaa tacatcatct ttttcatata tatctttaat agaaaaaaat
    25321 tcatactctg tagcatcatt agcattatac aatctaattt ccttgaagtt agagacaata
    25381 agccatgtgc aatttcttcc atatttaggc aagtaagaaa acccttgttc tactggtgta
    25441 ttatgattgt tttttcttag ttgttttgca tctaaattag ttgttggttt ttttaattct
    25501 attgctactc tatagtcact ttcttcatta gaaaaatagc ctagtacaat atcagctctt
    25561 gttccatcaa tcttagtttt ttcttctggt tttatattat aatccattat attgtccgta
    25621 agacttttat acctaagaac tttgtttaaa atatccgata caaatatttc cttaagttga
    25681 atttcattta atttatcaat agatttatta tcaattatat ctctccattt ttctaaaatt
    25741 aatcgtcttt gtttatagtc atataagtct aggtctattt ccatattata tttttctttt
    25801 aatagttttt gattaaaaag atacttttcc atacaattcc cctaattcta tttaaatagt
    25861 ttatattttt attatatcaa gtgtaaatgg taaaatatac caatttacac taaaatataa
    25921 aatcattcat tagataaaaa tatatattat ttgtaataaa tgttagataa aaggaattta
    25981 ttcttggtaa cacactaata aaataataac tatctgttat aataaacttt tttattctat
    26041 acaaaaaaga cagctatata agctgcctta atatttttaa ctttttcatc aatatgtttt
    26101 tcaaggtgtc agatacatct ttatatccta ttttttctac tacatctttg actacagatt
    26161 aaatctcatt atctatcatt ttttataaat aggtaaccct taaacttaag gctagttaga
    26221 agctacagaa tttaattcac atgataaacc attccagtaa atcatctacc ctcaagtagt
    26281 agctcttcaa tcctatcaca ctcaaagtag tgaccactca aacatcaaaa taagctctta
    26341 tggtggctaa acaccaaaca aaatgttcct ctcaggaaca cttgttgaaa ttccaatact
    26401 ttcaaccata aaaatcaacc tcctatattc gattctaagc aataaagata tatatgtctt
    26461 tcaatatttc caagtgtctt atatttgtca tacacatgtc atcattgaaa taactacaac
    26521 ataaatgatt caatttaaaa aacaatctaa attgattatc tttaatattt attaatacat
    26581 cttgatattt atattacaat agaataaacc ttctattatt ataagataat agaaggttta
    26641 ttctatctcc acaccttaaa ggtgtactat ggccactcgc cttatctata atattttaat
    26701 aataataaac gtcttataaa ttttacaatt taatctctaa aacctatctt ataacttata
    26761 cattcatcat ctacaaaata aattatatca ttgaacataa agaagctata atcatttttc
    26821 tttgcctgat tagccatttt tattatatct ccagtccaaa tatcatcatt ataattatat
    26881 attaaatgtt ttggaatatt gactttatta tttgtaaaac tatctttttt taattcttac
    26941 ttttctatta tcttaccaca agattcccta aaatgtatat taggataatc taattctctt
    27001 gtaacttcta atacttcctc gatatattca aatttaaatg ttaattgaaa caactatacc
    27061 tctctcttta tatcaatatg ttatatacaa gtatatttca caatatactt gtatatattt
    27121 ttgcatcaat caatgtatta gaacattgag aaacaattct aaaccactta tctaagcaaa
    27181 tcaactaaac tacctatcta ggctaaatta ctcactatat gttagagcat caccgctttt
    27241 taattttcca cttatgatat acatctacta gtggataaca tacaaccctt agaaaactct
    27301 ataacttaat agacactcga tattgtctat tttactggca cgcctaattc attcatttta
    27361 gacttaaaac actttggtac tctctcttgt gccttagaat tactttcctc cattatattt
    27421 gttctaggtc taaatacagg attatattta gtacttccat gtccccacac acctccattt
    27481 tcaaatcgct ccattggaaa agcatgtgct ttgctatata tatcaaacca gctcccacta
    27541 tctttaattt ctgtagctac agaatctgat gttatttcag acacatctat tgcattaagt
    27601 aattcataag ttctatcata aagtgatgga gcgtaatcag aataaacttg tttgtttact
    27661 tcttctttta ctatctcttt catttctttt ccaacttcag gcatagatgc agcaatctta
    27721 ttttttgtat aagcaaccaa ttcatctaat gatttaaata ccatctaatt cactaccttt
    27781 tctttaatat tattcatttt ttcaatttct tcagtctctt ttaaaactct gtcctgtaat
    27841 atctcattct tcatctgacc tagcttaatt tcagtattca ttaataaaga tgccgttaat
    27901 tcattttcta tttgacctat gtaatacatc acattttcta attcactaga tatattgcca
    27961 tctactaaat cttctatttc aatatttaac tctatattag tcaatttatc aatcaacatt
    28021 ttaagaatat cttcttgaga caatgctaaa tcaggattct ccataccaac ccatatttta
    28081 tttaatatct cttctttagt ctccttatca ggatttctaa cttctatata ttcctcttta
    28141 ccattctcat ctataattgg tattatagcc ctaacttcat cttgtattaa tttatctact
    28201 tgtattttat tcatatctat ctctccctta ttttattatt ggatatttac acttaataga
    28261 aactttacag tttccagtaa acttcaatat atttacacct cttcttaacc taatccactt
    28321 tctattaaca atattaaacc tattctcacc agaactattt aaaacagtat agtataaatt
    28381 atctattgtt attttttcat ttaattctaa tccacttata actaaattgt cttcttcatt
    28441 atcgctgata gtagaatttc taatagttat atctccttct tttagacatt caatatcaat
    28501 tactggagca tattcttcat ctacattaga cacattattt agtttaattt cccttgtatc
    28561 cttgacagtt ataacttttt gaaagcttct ataagcataa tttgtatatg gttgaaactc
    28621 tatttctaat acgccttttt tatcttttgt atatcttttt attatttttc tcgctttgag
    28681 atagtatatt tcttctatat tgtcataact gataaaaggt ttaaattctc tctgccaaaa
    28741 ccaattacat atgttttcta gcgtatagtc atcccattcg taagcctccc catgttcgct
    28801 acacaaacag aattgtagtg ttataggttc aatagtagtt tcatcctctg agtaacaagg
    28861 atttttatta aaagtactat cagatttaat cgcttcttcg tagggcaatc catattcatt
    28921 tagtatttca tcatcaccta aagtaactaa tttaatgttc atggattcac ttgaaatatt
    28981 atcaaataaa aacttatcat ctaaaaatac cactttatca ccttctttca tttttttaag
    29041 ttcaaatccg attaaaacca gatttaaacg taaaaaaata acccctactc ttttgagtag
    29101 ggaaattcac tatttcttag atttaacatc cttttcgtct attaattcag cacctaacat
    29161 agaagccatt ctaacagggc taacatcttc atctccaaca aaagcgaata ctacatattc
    29221 tttattttca tctggcaaaa catctagtgt catattaaat ccagatggat tctcagcagt
    29281 aagatttaat tctatacttc tttgtgcctt tgcatttggt atagtcatat ataagaccat
    29341 atattcacca tcagcagttt tagccataac atctgcttct attacatagt ttggagcagt
    29401 agactcttct tttattttta taacttttgc ctttggtaat tcctctaagt aaaacactgc
    29461 tactttatct cctgcattaa aatcagctgt agatattgtt atttctgtgt tagagcctgt
    29521 tacagttgaa gtaaactgta atttcttaac taaagatata ccatcttttt caacactata
    29581 tgcagatata ctattagtaa ctggttttac attttctaaa gtaactttct tagtaccatc
    29641 agaagttagt aattttcttt taccaacctt agccgattct tcaaccatat cagaagctaa
    29701 tagcattgct agttgtggca tttgtattac ctcttgttca agtgctaaac ttgctgttat
    29761 agctccatca aatgctattg tgtttgcacc tttagctttt gcgtacacgc tttctgaatc
    29821 taatttgaaa ttaaaagcat ttaaatctgc tgtataaaaa agtggttctc cagttgtttt
    29881 attttttact attatattac ttgcatcttt tatcgcaaat ctcattattt catcatcctc
    29941 tcattttttg cataaaaaat aacactgtta tttaacagtg ctagtttgta tttttatttc
    30001 tttagaccaa tgtttaatct tgtctgcctc aacttgatat ttaaaagaca ttgcaaagcc
    30061 taaactttca tcataatttt taaattgtat cttagtctta taagcattaa ttaattgcca
    30121 ataagtcatt tttagaataa catctattgg tacatatcca tctatgtttt gaactacatt
    30181 tatataatca cataagtgat atgcttcttt ttctcttttc tttctttcct tctctttaaa
    30241 gtgtgcaatc aactgttttt ctctttcaga gccagtattt tcaatccact tatcttcatc
    30301 atctttaaat aaattctttt tatcaataca aaacatctct aaaatcaaat ctgataccag
    30361 tccaaaatcc tctctactta taaaagcatt tttcttttta tcatcaaatt taattaaaat
    30421 accaatttta atattattca tatactcaat atttttaata tcagttttat ataatataga
    30481 taaagatttt tttatcttat cactaagtaa ctcaatttct tcagaagaat ccaagatagg
    30541 tatagcatca tatttacatt tcaattcaaa tattttctcg ttacatagct gagaatagct
    30601 cttttcaagt actaaaaatg gattaactat ctctaaatta gccatatcaa atttaataag
    30661 ttcttcaata gtaggttgat atacagttcc taatgtttca cctagtttaa ttggtaatcc
    30721 tgtgatataa taattttcca acattacata tcaccaaaac tttcagctga tacagttata
    30781 acaaatccgt tataatcagt atttaaatta tacatttgat atacattttc taacttaatc
    30841 ttacctatac cagctatttc ctcattttga gttacaatat caactataca acatactaat
    30901 gcaacatctc ttaagccatt tgctgtactt tggcattcat catgacaaac aacaccaatt
    30961 tctatctttg tttttttaat tgtcctactt ccttcaaagc atggcctata ttctgacatt
    31021 attataaaaa ctgaaatatc tgcttctttt aatacttttt ctactcttct atttagaaat
    31081 acttttttgt ttctaagttc tttaacagga ttttttatat caggcatgga taaaatatct
    31141 ttttgggatt catcattata aatcataaat ttagcgaagt cttgatttga cattagagtt
    31201 gctcctatat tacttatcat cttgttaggg aaagccatat aaacccatat atctaccccc
    31261 ttagtgttat ttttttagta tctattgttt tatttgtatc tttatcttta gccaatagca
    31321 aggcttcaaa gccagaatat ttagccaatg tattagcctg tatgacacat tttttctcat
    31381 cttgattaat tatattacaa tatccgtatt gcttgtctaa gagccattca actccttgag
    31441 aatggtctat tttgtattca ttttcttcgc ctagattaat aaatccatca cccataattt
    31501 tactaaaagg aataatcaca ctctcatttt cagataaatc attccaagca atattattta
    31561 ttgtatcatc ttttgaaatc aatgcatctt gtaaacaaat agctttaata agaccatctg
    31621 cccctgtttc tctgccatta tactcaaagt cattaatact agtaactcta aatacagttt
    31681 taccagttaa cattattcta gtgtcaatat ctatagtttt agtgacagga ttacttccaa
    31741 aataaagctg tctttttgta tctgagatag aagtatattt attatctgcc ataccatcag
    31801 aatataaggt taaattttca acagctatag ggattttata catctgacct ttatatttat
    31861 aattaaagaa ttgattgcat ctttttatta tgaaatgttt atatgtatcc atttctttat
    31921 gttctttaaa tatagttaac caataacaat tatcatagaa aagataacaa cccacatcta
    31981 catctaaatc agattcaact aatagatttt tttcatctaa agctctttta tcattattag
    32041 ttatatcatt aattgcaact atagctcttt tagtatcttc tgttatacaa atttcatcta
    32101 tatcagttat tggtacttca atacaggttg gtgtttcttt tagatatttc ttaaaactta
    32161 atctaagttg taatattctt ttttctttag gagttgaaca accaagtcca attctttttt
    32221 ttgtatcact taagtaactc atgctagttc catccttcaa aattttttag catgtatctt
    32281 tgttgatatt tattaaatct agttctcaat tgctcttctc ttgcttgaag tttagccaac
    32341 atattagcac ctgatttagc attataatca gaatcagtta gcatctgttt taaattttct
    32401 tctctattta tcttaggttg taaccaatgt atttgcatac catatgataa tattttaatt
    32461 tcatctaaat ctaaatcaga tactatttct cctaaatatt tgtatttgaa aattatctct
    32521 tctgtagttt ctacaacaaa acttattata aatttttcat cttcaaagct tattgtataa
    32581 tctttatcta attcgtattc ttcttttgta tcttttccca tcaaagttat ctctgcattt
    32641 ttattactat aattagatgt ataacttaat tgagcagcag gaataatttc tccacctttt
    32701 tcatcaacat attttatagt caaatctttt ttacattgat taaaatcaac tatagcatct
    32761 tctaaataac tgtacatcat tttttctatt atttcatttg atagtaataa catctcttca
    32821 tcattaatac catccaaaaa ctttttatat atttttacaa gtggtgtacc caaatattcc
    32881 accaccttta actaaacaag tacaaatctt ccattccaat tcttttagct aatagtttag
    32941 cctttagccc agaatcaaac tttttctttt tatgtaaatc tattgccctc tcagctaatc
    33001 ttcttgttaa ctcaacatta tttttattta atattttttc aaaagaattt atatcaactt
    33061 tatcagataa gaaatattct aaatagtctt cattaggatt tcttatatat tcatataaac
    33121 tattcaatcc tagaaaatct attatgtcca ttggagtaca ttcaacatct aaagaatcta
    33181 catccgaaat tattaatcta tgttcttcaa aaaaacctgg acttcttctt actattctat
    33241 aaagagtatc taaatctata aattcagttg aatcttttga acctacttta gataaagaaa
    33301 aaatctgttg tttttctctt gggtcaatat aatcaacatc ccatgtggat atatttttta
    33361 tttctatatc tatttcctgt tttttttgtt ttagttttct ttgtagttct aatctattta
    33421 atctagcttc ttttttattt aaagtagact ttatatcttt ttctaaagta ttaactttga
    33481 cttctgtttt agaaatattt tcttttttag ctcttgccat aataatctcc ctcccataaa
    33541 aaaaagaagg tggataacca ccttctaatt caattattgt attttataaa ctccaaattt
    33601 agcagcagtt acaactccta aatgtatttt ttgagccata aaatattcta tttgttggtc
    33661 atctctttct ttttcatcag tattttctat gaccaataca tctccttcag taccaagttt
    33721 gattatcttc tctccatcag gtattacata gatagcctta ttatctaaag cccactcttt
    33781 accttctgct gcattttcat cataagcatt tggtaaacta agtactttat atccacctct
    33841 aaatgtttgt ataaaaccat attttctttt ttcttctttt tctaaatcag tttgaacacc
    33901 ttctaaatta cttaatgcaa gtggagtacc atatataata ggctttttac cagtagcccc
    33961 ttctacctta gctatcaatt ccaataatac tttgtctgaa ttacctgtag cttgaaactt
    34021 agcatttatt gttgaatatg caccttcaaa tgtcttcgct attcttctag caatatcata
    34081 atgatatgaa tctgataccc tattcacaaa tacaacccaa tttattcttc ctgttataaa
    34141 tctctctgct tcttcatata tagcaacaga taatttaaat gctgttgtag gaactttttt
    34201 attaagtaat ctttgtcttc ttgtactatt aacaccatct gctatatttg caactttaaa
    34261 taaattagta ttttcaacag taaattcttt tttatctcct aaatcaaagt tctcaaaatc
    34321 acaaaacgga ctaaatactt cctcatttaa tctattaata tcatcagata ttaattctga
    34381 tattaaatta taaaatcccc atttattttc ttggaattta taataattat atttttctcc
    34441 tcccatcatt tcttttaatt tattctttat tgcactttca gcatctctat atgttattgt
    34501 gtttccatct ctatctgtat attctctaaa tgtatctgta tatagctcat gagctattct
    34561 ctttaaagtt tttgctgttg ccattaattt acacacttcc ttttctattt ttttgcataa
    34621 aaaaataaca gttatagact gttatctcct gtttgttaat tttatttgat tttataaaac
    34681 ttttattgta accgaatctt gaccttcata gttccataat tctaatactt ctgctactgg
    34741 agttttagca tctgctgtag gcaatttttc aaactgaaac gaatcagatt ttaattgtaa
    34801 cttgtctttt actgcaatac ttttatctgc tatatgtaat agagatatag ttacctcatc
    34861 tccttttttt agtcgtctac atctacatat ctgaccagga gaaacttcgt aatctctttc
    34921 atctaatctt tcatcataca tagttgctgg gtcatcaact ataccccaat ctaatgtgtc
    34981 atttgtaact ttatatattt tatgggtatc cctaccatac tccgtgtctt caagttctcc
    35041 aatatttatt acagcaccat tttctaaatt tattggattg gtatcctctg atggaacaac
    35101 atatttacct gttaaaacat caggattttt tactttatct gtttgtatta ttgctttatc
    35161 tttcttcatg taaattcctc ctattattct ttatctgaat atttttttat aaaagatatt
    35221 tcttctggtt cactaaaact atcattatta ttacttatag gtatttttat cgtattattt
    35281 ttatcatcat ttatactaaa attttccttt tcgctttgtt tattgctaag agtttttaat
    35341 gcaaatatgt aacccaattc tttcttgtat tcatctaatg taatttcctt attataagct
    35401 tttattttta tatctttaat ttcatcttct gctaattcaa atttttgagc tacatcatca
    35461 actttagatt taaataactc aaattctttt tcttctttga atgcctgtaa tggctctaaa
    35521 ctggctttta aattagatat ttctttttgc ttttcagtaa atttctctat tatttctttt
    35581 tttaattttt cttgtctact aaaaacctgt actatttctc cttcttgttt ttccctccaa
    35641 gtttgtatat atgatttttt attttcataa tccaaaatga cagcatcttc attaactata
    35701 tatggaatac cataataata cccccagtta atttcatctt caactatagc aatattctct
    35761 tctggtatta aatcattata ataaaaagct tgtacttcaa ctttttcacc ataccaattc
    35821 tcagtttcga ctttgtattc ttttaactta tttcttattt cagtactaag attactcact
    35881 gctaaagcat atccttcaga atttttatta agtttttgat tcttatttgg caaattcttt
    35941 tccccctctc catgttctaa tgcataaatt tctgagcata tttttgatat ttcttcttta
    36001 taattaattt ttcttgaaaa tttagttaaa caacaatttg aacccatagc tccctttaca
    36061 tcagaaccaa gacatgtaat tccatgatat acatagtctt ttatattata aaatccatct
    36121 gaatcatcta attgataatc atttatatct atttccattg aaatttctat ttcatcagaa
    36181 tccataatta tatcgtaagc atgattgcta tatgatttat atatatatcc tttacattta
    36241 acatacttct gcccatcaat ttcaactttt tcatatttat ttgtttctgg tataacgcca
    36301 attattcgtt cttcataata ttcttctaat ataaaccctt catctgtttg aactacttgt
    36361 gttaaaacat tatgaccatc aaagtcatat tttccatctt catttatttt tacatatcct
    36421 agaattggaa cattttttaa tgttggttca gctttatcaa tagcttcttc ataaaattta
    36481 gttccatttg gatttgtacc ttcatgcata acatagatat acactggcaa aagattacta
    36541 tttagttctt ccaatttttt tttatctgaa taatgtttta aattacaagg taattttaat
    36601 atttttattt ttttcacctc cttttaaaaa cgtattttgc tagtaaatat aaatcttttc
    36661 atttcttcat tactgaaatt atttattaat tttgatttat tttcaaaaac atataaagtc
    36721 ttattattca acttgttttc acatataaaa cgatatcctt tttccattaa taatttctta
    36781 tcatcttcag aaaaagcata tataaactta ctcataatta atcattctcc ccatttcctt
    36841 ttgcttgagt agaattacca ttatctgtcc ctatatcaga tttatctggt ctaccactgc
    36901 tttgagtatg agatgtttgt tgaggtatta aaaactcatc aaatcccatt aatttttctg
    36961 cttgtaaagt gtttatagct tgaagtggag taaacccagt acttgcaata aacaagcttt
    37021 tactccctcc gtatcccata tcttctcgat actgtttgga taaattcatt ttattaaagt
    37081 gagtagttcc aataaacttc atattaaata aatttgcctt cttgtcttta tttagttcaa
    37141 agttaaccca attttctatc atttgttgaa tcctcatagg aattaagcta tctgcaataa
    37201 taccatttaa gatagattca ttatttatct tgtcagagtt aagtaaggct gtatttattc
    37261 cagcgttatc aaatatgaac tcttttgctt cttttacata atcatttatt ttagattttc
    37321 catctgaaag agtatgtgtt tccaactcta aaggatttgt agttattgct gttccttttg
    37381 gtaaatttgc ttttgttgaa ttgtggtata tgggaattaa attaaggtct actagtggtt
    37441 catcattttt gccaaatggt atcttttgat gtattaattt aatactttct attactgcat
    37501 ttgtgctttt taaatctttc atatcttcaa gctccattaa atcgtcaaat ataaagcaaa
    37561 aaaatggaac tcctttcact gaatctaaat cataattaaa tgctactgct ttatcactta
    37621 attgaaaata tgtattatca attaattctt ctcgtgtaag taaattagat ttatattttt
    37681 tatatatgtt tttaatttct tctggaaagg atattatgtt tttgtcagta agttttttta
    37741 tatttatagc gtatctcatt acattatttt ctatcattgt tatcatacaa aattttgctg
    37801 gtatctgttg taaaaccatc cctgaattat cttctatctt atataaataa acctctccta
    37861 attccaatac tttttcggtt atccaagctg atgtaaattt tatattatat ttttctaatt
    37921 gactcgccga attcatatat gcttttgtta ttttttcttt tgtttttaat ctacttatgt
    37981 cttttgggta taaaatatgg tcaaaagtat tcatattaga tattaaattt aaaactcttc
    38041 tatatgtacc agatgtagct tttaatatca tacttgcatc ctgaagtata tctgtatttg
    38101 aaatttgata tggattggag agagcttttc ttaatttgtc tctgcttata cttcttaact
    38161 tatacatttc tgttgctaat gttgtggatt cttttactga ggcatatttt ccaatttgag
    38221 catttagcat attgatttga ttttgtgtag aattgttgtc tttagacttg cttttattat
    38281 tatttttttt gcccacttat tcacctcatc tcttaccaat aaatcataac agttttttga
    38341 ttatttctat tttttctttt ctctttttct tccagataat ctgctaagaa attcgtataa
    38401 gccaaactag aatatctatc ttttcttgct ccactttttt cctttatttt aatttttcct
    38461 ccatcagcac tatattctaa atttattaac tcattcacta aagccgttgt ttgaagataa
    38521 ggggcaatat attttgcttc taattctgcc tttttatcta tttcatctgc ctttatcatt
    38581 tctttttcta atatcatttc ttttgcttca atatcactta taggaagttc taaggttcta
    38641 tttgtaagga catctcttaa agacatagcc attttatgat tttcatcagc gtaagctttc
    38701 atggaatata caacaggaag tgattttttt gctagtgact tgtcaacagt attatcttca
    38761 ttaaaacatg tgtaagcatc ataccattca tctctatctg aatcataatt tgctttctgt
    38821 atatagctcc atacagttgt tcctatacct tgtgtatcta atatcatata atctgcctga
    38881 aaatctgaaa ataattgttt taatcttatt gcttgttttt cagcttccat accattataa
    38941 gcttctatat ggacaacaca tcttttatat gtgccttcat ttggaagcat cctcatcaaa
    39001 gtataaatag aattatcatt cttaactcct ctggccaaag ctatatctgc accaataatc
    39061 cgtatttctc cttttatctt attaagatga tatttcttat ctcttttttt cttattttca
    39121 agatactcta tatcactagg aggataaaac atactttcta ataccctaca tggattcacc
    39181 caagatgact tgaagaaagc atcatctgat tcattataaa acactccaca atactccatc
    39241 atgaaagaag catcactcat actctctttt ttcatttctt taagtattct ttttttagtt
    39301 aatagtccat gttctaaaga gcaagtgaat ggaatactac atgcaaacat atcttcacca
    39361 ctaagcatac cttttactgt ttccataaac tttttataac tccaatgctg agaataccag
    39421 cctgaactaa gataaatttc tttattctct tcttgcatat atctatattt aggattctgt
    39481 aaataccctg gttgtcttgg gtttgttaaa aatggaacta aaacatcatt cacagttcct
    39541 tcatccacca ttctatattc atcaacaata agaacattag ctctgtaacc tcttgcatca
    39601 tcattagata caacagcttc tattctactt ccattccaaa aatctataga tacttcttgt
    39661 gcatttactt ggaaactttt aatttctctt ttcagagtat ctgattttaa atataaatca
    39721 cctaaaattt tacttgtaat aagtaatttt gcttgtttct ttctttttgc tgctattaaa
    39781 acacaagtac ctggttttaa tacagctata acacaacaaa acactcctag aagccatgat
    39841 tttcccagtc cacgagaagc aataaataca aattggtcag acttcatcat catataaatt
    39901 aatatctgtt gaaataaatg tagattaaat ccaaagtaat ccatacagaa tctatgtgga
    39961 tttgcgagat aaaaagcacc ccaatactct cctatactct ctagtagatt tcgggatgaa
    40021 tcattagatg acagctctct tttttcatat ttagtcctca tttttattac cttctaaaat
    40081 gtctcttaca tcatctttaa atttaatatc tttatcgcca tcatttatcg aatacttacc
    40141 tgtagctaaa cctaatgcaa cagcaagtgg ttttttcata tgtttattta cataagcttc
    40201 aaatccatct acatcttcat attcctttaa cctatccttt acaggttgat ttttttcata
    40261 tatcatcatt ttcattccaa atgtatcatc ttgcgaatct ccagctttct ttttctgact
    40321 aggctttatt tcagcatctt gcattttact tgatactaat ttagataaat cattaaaagc
    40381 tttcatatct cccttttctc ttgatttatc tttttctaat tcaataatac aaatctcttc
    40441 aaacaatttt ctttcagtta atgactctgg ttcataatat tctttatatt ccttatattt
    40501 tctttctaag attttatact cataatcatc aaaacctctt ccccatttta atttaatttc
    40561 atcatttatg actatatttt tatcattatt atttcctgta tatatttctt tcaataaatt
    40621 atctaaagat gtctttccct tatattttga atttctatta ataattttca tatattcatc
    40681 tattaattca tctttatttt ttttatctgc aagttcttta gctaattttt catcaaaata
    40741 aacatcaaag ttgaaacata tatgttttag tgcctttaca agttctccat tgtaacaccc
    40801 atttaatagt aaaaacctag ctctaaaaca ttctttgcat actggaagtc ttttatttaa
    40861 agtaaatatt tcactatgac ttttccaaaa atatctatct gtatattttt cttcaccaca
    40921 attcaaacac tttgttttac taattgccat tcataacatc accaccatta aaaaagacca
    40981 ctagagaatt aaatctctag tggttaatag ggagattcaa ctcaattaag agctgaatta
    41041 aatttacaat ataaacttaa ctcagctctt aataaataac ttatagctta cttaaattta
    41101 tattgtaagt acatattctc ccactttcat taaatatcat taatttttgc attgcgtgtg
    41161 aacttaatct tagattctta gcataagaat ccgttccgga gaaacttcca tttacacata
    41221 tttcactttc tccataatta tcttccttac aattgtgtag atgagccata aagatataat
    41281 caggaattaa ttttataaga gaggtcagtt taggaattgc tgtagacatt ttatctttgt
    41341 ctccatgcac tccaaaacaa gtatttccac atattttagc cactattatg tcattatcat
    41401 atatattttc ttgaaatata acattgttta tatttttaat tcttagcttt atatattcat
    41461 ctattaataa tgtaaaatta tctttatcta aattttcatc ttttttagga agaactctat
    41521 catggtttcc acctactgaa taaataataa ttttatcaat ttctttagat aactcatata
    41581 taaattcact aagtatttca gacacctcta taatttgttc aactatattt tctctatttt
    41641 ctaatcttat agaattatgt atgtatccag aaattaaatc tccaagtaac attacatgca
    41701 gtctcttaac tttatgtaac ttactatatt caataatttt atcttttaaa tattgtattc
    41761 ttatcttgaa tatttttgaa ttatatctat taaatgcatt aatagtctct aatccataat
    41821 gtatgtctga aattagcata atagcctctt gatttgaaga ttcatagcat ttgtaactag
    41881 aatcacttag aaatggctta tgaagtgata tatcatctat tttattatta aataaatcaa
    41941 ttatattatc tattcttgaa tattctctta ttttcttatt tactaatgac ctttcatcag
    42001 atagttgtac ttttatcttt tttaattcta atattttctc atttatttta tcaatctctt
    42061 cttgagtaga attattctgt atcttagaat taagataatc aaaagcctct ttcaacccat
    42121 aggaaacctt cctacaatgg tctggagaac attctaaccc caatagttca actatctctc
    42181 cccaatccaa atctatctct ttattaacct ttgctagtcg tattctaatc ctatagtcaa
    42241 ctagtgactc atcttctttt ttcttcaata tgtcttccat atttaatatc tccctattta
    42301 tcacataaat atcattatca atgtattaag taaaaaataa atagtccaat attgatttat
    42361 tggactattg tttcacttaa tcttatcgtt atgtttaaat ctgtatcacc agtatactta
    42421 tccaaaacat cactaagtaa tacttcatgg tctccatctt tcaaatgttc tataatttta
    42481 tatgtattat tttcttgttt tattatttca catttttcta atactgttcc tttattattt
    42541 ttcatgcact ttcacctttt ttataatttt attaactcta ctgggaatat atttatttgc
    42601 aaattaggca gccttgtagc taacaatagc taatataagt taacgcattt agcatttatc
    42661 aaggattttc tataactttt ttattttatt tttactattt taataattta atcttttatc
    42721 cttgagattt tatatttgaa tttttaactt taagctttat atctattgtt ctcttttata
    42781 cagtacaatc gtttcgtttg tatgtaccca tcacttttat ataagcagtt tgatttttaa
    42841 cccaaaacca aatgggaact tataatttat gttattcgcc taacctgcaa attaaaaaat
    42901 agtccaatta aggactatct taaatctaca tctataattg taagtgcatt tgatgtactc
    42961 aatgcaaaat caacttcttt ttcaaattct tctatttcat cccttaattt ttctattttc
    43021 tctcttactt ttaaagggtc tactaaagac cattcttgtt gttttttata aaatgaaata
    43081 agttctgctc catctttact tttttctttt tcctcaaaca ttctgtctgt atttttttgc
    43141 acttcttcat ttctacgatt tacttcagac atcatacaat catagttact aaccatttgt
    43201 ctcaacaagg atttatctaa ttctatacta ttttttcttt ttattgcttc taaaactgta
    43261 taatttttat tactaatttg aacatttgtt atagcattag agatattaat tgctttatct
    43321 aatgcgttat aattatatat taaatcttct atttgttggt atttacttga aacttctaca
    43381 ttaaaatctt ctacacttgt ttttgtttca tatacatttt tatcagaccc ttttttagtt
    43441 cctacaattt taaaattatt aatgcatcta tctattttat cttctaataa cttcttttct
    43501 gctaaagcct tgtgtacatt atatttaacc ataaaaacat ctccctttta attaaaatat
    43561 tatttaaaaa taacaaactc tttttattgg aagagaggaa gggatttgaa ccctcgtatc
    43621 aaattaattg aactaatagt ttagcaaact atcctcttta ccgcttgagt acctctccat
    43681 atttgctagg aataatagga gtcgaaccta tgacctttcg ggtataaacc gaatgctcta
    43741 accacctgag ctatatccct tttgaaaaca aatttaaatt atattaccaa tttaaaatct
    43801 atatcatttt cctctaacca gcttttagct atcttttcca tctttaagat ttcttcatct
    43861 gaaaatctag ctaatccttt accttttcga tttctaacat taatgccaag tttatagtta
    43921 ataaatctat aaaattcatc ccatgcttta ccaaacatac aattaaattt tttaactgct
    43981 attgttctaa cacatttatt aataatagct ttactttctg ttatagtgac tgaattagtt
    44041 ataacatctt ctaaatgaga tattctttta ttcttcaacc ctattaattc tgtctgtagt
    44101 acagataatt tattatggtc accagaaatt atcgcttcaa gcatgtcttc ttttatattt
    44161 tgttcatttc taatttcttc tattacaatt tcagtttgtt cttctgcatc atgtacaata
    44221 tctagtaatc tagttctaac ttctttagca acttctgaat ctctaagtaa catgcctatt
    44281 ctaagaatag ctcttttagg tattaatatt agacctcttg gaggaatttt aaaagtgaca
    44341 tccagtccct ttttagtatt ttcaagtact tcagctatat cattgtattt atataactta
    44401 agaccatcta attctaattc aattctattt ctctgtataa gtttttttat tgtatctcta
    44461 tctacttcat aatactcaga aacaagttct actgtactaa aatctgtatt tgctaaagtt
    44521 aatatctctt tcacatgttc caatacatct actctatcta tatttctatc tcttaattct
    44581 ttattttcta taagtttttc accattcatt ttatcaaatc tcccttttag tatttattta
    44641 aaaggaagaa agacttactg ctatctcacg acatgtgctt tcttccagtt ttgaaatggt
    44701 gagtaattat cccacaaatt tcacgttgta gtttaatatt tttagtggca tcatcatctt
    44761 acaatataag atacctatta ccagccacta gagcttctca aaatgagcta tctctgctaa
    44821 ttgtattaat ttttagcata ttctaggttt gctaccatat gtcaccatat gaaagggctt
    44881 ttaacccata cgctctctat ataattgcat cttttaaaga tttagctatt tttaattttg
    44941 gaactctttt ttcctcagtt ttccaaggtt ttttagttcc atctttaaga gttatttctc
    45001 cttctttacc tttttgtatt ttagttaggt atttaactcc catcaacgtc aattcttcac
    45061 catcttgtag tacttcgttt cttttttttt ctatcacatc aagtatctct cttactctta
    45121 tctgtgatac acctaattct tctgctactt cttttaaaaa atctactttt ttcatatctt
    45181 cttaatctcc cttttcttat ataattttca tttgataata gtaaggaata attttattat
    45241 ttttattaac cccttacata agtaatatgt tttaagcgac ccttttttaa accacttttc
    45301 cccaccagct aattacagtt tttacaattt gagcgatatc cctttttacc atttttatca
    45361 aacctagata gcaacttaat ttttccacat ctgctacatt ttttatattt tcctttacaa
    45421 acagatatat aataataatt ttcttcatag tttttctcat atgcatctat tattttgttc
    45481 actattttat ttatttgatt atttattgct tgaggagtta tattcattaa atttgaaata
    45541 aactctaatg atttatcttt tctatataaa tttaaaatat ctttttgtac tgtggtaaat
    45601 tcaattttag atataatatt atttaaatcc ataactatac atgttaaatc atcttgcaaa
    45661 tcattaccct tttgaatcct taaaagctct ttaacatgtg atttatcaaa catatctaaa
    45721 tagtcccatt ctgggcttcc tgcatctgct aatggtgctt tccatataat tggtctctct
    45781 atagaatttt taacatcagt catatcattt tttaatgcat ttaaattttt cctaaccatt
    45841 ctcttaagtt ttaactcttc tttacttaat tgtttatgtc ctaattcttt atatttagct
    45901 ttataccatt cataagtatc tgagtatgtt tttataattg gatatttttt aatatcttta
    45961 ggtttaactt ttatatcttt tattttttta tagtttttag gtaattgaaa aaatgcaaag
    46021 gaattgtctt ctaggaatcc atctttatta gaagtgtttg aatttatatt tccaccattt
    46081 gcaggtgcta ttttatttag aagtgcttct tcctgacatg ctctattaaa taactctttt
    46141 gaattgtata ttcttatttt agtttcctta ggtttatcat ctactgagag tatatagctc
    46201 cccattattt ctaatgtttt tgctatatta gaagtagaat atatactgtc atctttattt
    46261 aatatcaact taattttcga tgtattatga tgtgtgtact cccatgtacc attactatta
    46321 ctaaaaggat ttttaacatt tatatattcg attggattaa tttgattata gccacaccaa
    46381 gtaatgaatc tattataatc cataactact tcttctccat caatagtaac ataatataca
    46441 ccatctttat ctaaaggact atctgatgtt tgctcaaata tattttccca aaatttatca
    46501 ttgaattgtt ttttttcttg atatttcact ctagtaataa tattctcatt tctctttgta
    46561 agagtattat aaaatgtatc tatataattt ctatttgact cttcaaacca cgtgtgtttt
    46621 acccctaatc gttcctctat gtatttaatt ctatctttgc tattgtctat gctataatct
    46681 aaattcttta tgtaatcttg tgcagatttg ccatctattt cttcataaaa attgttatta
    46741 ttatatatca atattatacc tcctttcctt gcaatccatc atcataatct gtatcatcat
    46801 aatcccatag ttcatcctta aaatcctcct tatgcctttc atttacaata tcatatgaag
    46861 accttttact catctctgtg tatgtatcta tcatttgttc atattcctca tttaatattt
    46921 gatacctatt tttatgtacg cctgttttct ctgattcatc taaatcttct aaatatatta
    46981 attctatttt tttctctttc atacttctcc ttttctatat tataagattt taaattaatt
    47041 ttcttggtca catttctgtg ccgttttttt gccttttggt cacatttctg tgccacccct
    47101 aaatttaatt ttttaactgt ttttcctata tttttgttaa tttttacagt tcattacctc
    47161 ttattcttag acagactttc taaatacaat taaaaaagca taaatcaatt gttttctttg
    47221 ctaaatttaa attatttccc ttccatatta cacaaggatt tataatgaaa tagtctgtat
    47281 ttttgttatt cccttcaacg attacatatt taaaaaaata atatttatca tcttctacct
    47341 ttatataaaa tctaagtaat tcctttttaa atttgttcat atcttgtttt attgtactaa
    47401 tatttaataa ttgacaaatt ttatataaac taattttttt tatttttgaa atatctgttt
    47461 catgagggtt taaacataag atattactct cgaaatgtat atatggtatg agttgatata
    47521 tataacttaa ttgtttatgt tgtcttggtg tgcaatgttc aaaaagaaat ctactagtat
    47581 ttatatatat tcttgtatat tctttatttt caaaattagg aattccttta gtaaaatact
    47641 ttggatttat ataaaattta ttattagatt catataataa ttcacatttt tttacgtctg
    47701 ctaaaaaatt tctaaatgcc ctatctttaa gatttaatat ttctctcatg ttttttctat
    47761 ctataggaat catttcttta aactgagaat atctaaccaa taaattttct tctcctgtat
    47821 tatagtttag atatgttgcg agatatatta atctacttat attagcttta cttatattta
    47881 cttcattaaa taataattca tttttaacat aagtacaaaa tacaaaacct cctaatttaa
    47941 aattatggta tggtaattct tttttattat tgataatagc cctttgctct ggagaaaaac
    48001 tcttttttct ctttccaatt tcaagtacct cacctctttt catcccttta tgctccaata
    48061 tttctcctgt tcttttgtct gttacaataa catcttttat aaattcatta cctctaacta
    48121 taaatatcta cctcttttct tataaatttt atttcaatta caagataaaa atcatcttgc
    48181 aattgttctt cgcaacatgt tgcttcagaa atttcattac cctctatgtt ctatttctat
    48241 tatatcatat accctctatg ttctcaatat tttttttaat atctacccaa aatttcttga
    48301 gtagatattt tcttttaatc tggcatatta ttaaatatat tcttaataat aggattttct
    48361 ctttttatat cttttttttc ttcagtaaca tttgtctttt tatttgtcct atatacacca
    48421 ttgcctaaac attgttttaa taatttattt tcttcttgaa gatttttcca ctctaatatt
    48481 attctttcca aagctgtatt tctactgctt atatttttat cattttgata cttttctatt
    48541 tcaccaaata tatcttcttc taaataaaag ctagatgatt ttttcatata atatcactcc
    48601 tagaattgtt ccatacctac ttcaaataat cctttagctg tagccatttg tgcatcttta
    48661 gaaactttaa atctattatc gaatgtcata tgtaatgctg tacctccact tacatataca
    48721 tccatttcat ctatgttctt ccatttagaa gatattcttt gagctgtgct ttcagaagcc
    48781 ataatgtaag caactttctt aagttcatca taatcattag aactatctat ctcattaagt
    48841 tctcttatta ttcctctatc ttttagttgc ttctgaacca ttgttaaagc atcttgattt
    48901 ccagcttcta atgtgtctga tagtttatca ttaaatacaa aacctttttc aaagaaagac
    48961 atctcagtac ttctaaaacc tattgaaacc atgccaaccg ctttatcttc atttactttt
    49021 ccttctaatg tatgaaataa tgcagcatct ccttctctaa aaattgatat atcagatatt
    49081 tttatttctt tatgtccacc ttttatttta tcttttactt taaatgtttt acctttatat
    49141 gtgtctacaa cttcttttaa tgttgttttc ctaaatgatt tatatggaac gcccatcatc
    49201 aattttactt catcagctac agctatttca ttaagagcag cagcaactaa tattctcatt
    49261 gtaaaagata ctttagaatc atcagaattt cttataggtg ttatactttc tttttctgct
    49321 aatgaaccaa caaagtaact tacactttca aaatctatgt atattggatt ttcatatttc
    49381 tcaaaatcta tgtttcttcc atcaccaaat actgatttga ataaacattc tttaaccatt
    49441 ccattgtact tactatatcc ttttacgtat cctcttccta tatctaagcc tataacttgg
    49501 taattttctt ccattttcat tcctcctaag taaatatttg ctaaatatat tataccatat
    49561 aaaaacttga taattacatt aaatttgcac aaaaattcaa ctataaaatg acatttttta
    49621 gttattagtt aaaattagca ctaaattaga actatatata atttaaattt aaaccaataa
    49681 tttattatta aaaattttta gttaatattt aaattatata tcaactattt ccaaaccaat
    49741 aaatcaattt ctttaactat actatttatg tttttttaga tgtcaatttt aattttaagt
    49801 aaaatattga aacttatttt cttacagacg acattttcat tctaatttaa taaatcttcc
    49861 tatatttcaa gttatttttt gtatttaata tttttatata tattagttgt ttttatgtaa
    49921 tcaatacata cacttttatt ttatattttc cttgttaaga atgtaattta agagtaaaaa
    49981 tctaagaaat atctttagat aaatatttgt tttatgagga aaatacagag ctatttcgtc
    50041 tgtaaatccg tctgcaattt ttttgaaatg tggaaatgaa aggactacac acaaaatcct
    50101 actgacaaac aagccacttt ccataaaata ccccctactt catttttatt tattttaata
    50161 tgtaaaacac tctatttact ttatttaatt aataaaagta ttacttttat cttattttaa
    50221 aaatctatgt ttttgtcagc aaaaaatggc ataaaattaa gtattttatg ccatttttct
    50281 aaaacctagt attttaaaca cttacaacac ctacaccttc tattttgtca gtaaaatgtc
    50341 agtcattata attcttagac tacaaacctg ttgtaatcac tgcatttcaa tttttagtta
    50401 ttgatatcta tataaaatat tatttgctat agaataaatt tttttgttat ggattataaa
    50461 gtcttatata catcatttta gcattaatct gacatcgtcg aatatcaact cacaacctca
    50521 ttgccatatt tttcttgaca aatttgtcat tattgtatat aatttaatta agataagaat
    50581 taatcttatc tatacaattc aatattaggg ggtgacgtta tgagaataac aaaaggcaaa
    50641 ataaaagagc taatcgagtt gtgccaccaa cttgaaaagc tctttgtaca agtatttaaa
    50701 tcacttgaac atattttgat ttctttatgt tcttgtatcg gttggttatt aatacttata
    50761 tacataatta aacattaata accaatgcaa tatacctata aaaatatatc tctataggta
    50821 tattgtaaca cacctaatat tgaaagtaaa cgaggtgacg acatgagtca acatattaaa
    50881 aacactttaa aaataataaa gatactaggt ttattattct tcttatattt tgcacttaga
    50941 gcaatatata ttgtttatct ttaatatctt tattgagttg cttaatgtat aagtaactct
    51001 tttttgttat aatattaatg aggtgatagc atgaaattag aaaaattaat tgatataaga
    51061 aaagaaaaaa aatattctca agaggatatt gctagaatgt tagacatatc tttaagaaat
    51121 tatcacagaa aagaaaaagg agagaatcaa tttacagtaa gtgaactaga aaaaatatgt
    51181 agctatttag atatagaatc aaaagaacta cttgacatat agtaatataa tctactcaag
    51241 gtagtactta acattaaata aaataattta ataatataaa atagactagc tataatctag
    51301 tctattttat tataataaca tattgtacaa gccatttaaa tccattctaa gcacatttca
    51361 atgcactcac cttatgttta tacctttaat ctttaaaatt aaatataaaa ataagctaga
    51421 gtatatatgt ctagcttaca gtagtttttt taatgtgttt atttgttctt gaactatcgt
    51481 tttactctta gtatctaact ctctataatt gtctaataga ttgacttctt cttcttttag
    51541 ttgtatattc tcttttaaat ctgttttaca caatatataa tctatactta cattgtatat
    51601 attagacaac tcaactaatt tatctatatc tatttttcta ttaccatttt cccaccttaa
    51661 gacactatta taagctacat ttagtttatc ggctatttct tgtatattat agcctctttc
    51721 ttttctaact tcttttagtc tattcatttt ctcaccttct tttttacatt tataatatca
    51781 tataagataa ctttaaacaa caccaaaaag gttagtacta taaaacattc aaaccctttt
    51841 cggtattatt ttttattttt tatattgaca tacacccttt ttggttgtat aattgaatca
    51901 tagcaaagga gaacaagaaa acacaaagcc acctctcaaa tgaagtaaca ggcaaagaac
    51961 cttcttaaaa ctctaaagct aatatcttaa aggattctat taaaaaatat taaactacta
    52021 ttaaaaaatt actaccttta agttgacctt ttaaatggtg tgaatggtga aaggcacaca
    52081 acacaaatat tacacatagt tttttcttca ttttttaact acaaaaatga attagtcata
    52141 ccagttggtg aggtgttccc aacattaagt aattaaataa ataaaattaa taaaggtttc
    52201 caattaaagc ctacaaaaaa ttgaggtatc aacttagttg cttctataat ggcactcaat
    52261 acaatttaat atgacatagc agttgtgagg tactgcgtta taaaaagtac taaagattga
    52321 gcattaaaat tatagtttgt actgcttgac acctacatgg ttataaacta tctaatattt
    52381 actaaaatta tttattattt attagattat tttagtaagt gttagaatac aacacttaaa
    52441 ttatgatata ataataaaat aggagtgtga acggcatgga tgaaaaaata ttagaattat
    52501 tacaaaaaat ggatactagt attaatgata tacaaggaga aataaaatgg attaatacta
    52561 gactagatag tatggatggt cgttttgatg ggattgaaaa ccaacttagt gatttaaaag
    52621 aaggacaaga agaaattaaa aagaaactag acttaactta taatcaagtt gctagaaata
    52681 tggaaggtat aacagaagtt ggcgaaaaaa tagacacttt aaaaaatgac atgaactttg
    52741 tagaaatggc tacatctaaa aattggaatg aaatagcaaa gttaaaatca attaaatagg
    52801 agtagataaa atggatgaga aaacattaga attgctacga gaaatacagg aaagtattaa
    52861 aattttaaat gacaaacttg acgaatttga ttatacacaa gataaaataa aatctaatgt
    52921 tgagggactt ttagaatgtt ttagtcgtat tgattttaga attgaagacc tagaaaatgg
    52981 tcaaacctca ttgtatacta agcttgatat agtgcaaaat gaaacagcaa aatcaattaa
    53041 atagcagtac ataaaaagac acttgaatta ttgagtgtct tttctacaag ctcttagtat
    53101 ggtattatta aacaataata tcattggagg tataacgaat gtatgagcag agaaaagcaa
    53161 aagtaatatt caataaagga gctggtaatt ctagaggaga atcatatacg aatagggtta
    53221 caattccaac tacatggata aaacatatgg acataactaa actagacagg gaagttttgt
    53281 tgacttttga tggtaataaa atagtaatag aaaaaataga tgagtagata aaaaatctac
    53341 tcaaaaaaat acttgacaac tcgtgacgag tcgattataa ttaaatcata aaataaaatt
    53401 ttttacaaaa tgactagtaa cgagtcagta aaaagggagg atataaaatg ctaaatatat
    53461 acataactaa cctaggaaaa tataacgaag gtgaactaat aggggaatgg gctgaatttc
    53521 cagtaaacga ggaagaatta gaagaaatat tagatagaat agggataaat gaagaatatg
    53581 aggagtattt cataacagat tttgaaacag atatagaagg gttagaaata agtgaacata
    53641 gcaatataga gcaactaaat gaattagcaa aagagcttga agagttagaa gaatatgaga
    53701 ttgaacaatt aaaggcttta cttggatgtg gatacattga tttttatgac atgcttgaag
    53761 gggatatttg cagattatca ggtaattata cttttataca acttgatgac aggtcaccat
    53821 tggatgatga aaatttagga cgtagttaca tagaagaaat atgttgtggt gatttatctt
    53881 gtatagaaaa tattgcttac tactttgaca tagaagcatt ttcaagagat ttacgttttg
    53941 atagagatat gattattgaa aatttatacg aagaagatat agaatattat gaaaatatga
    54001 cagatattga atttgcagag aattatattg aacgattagg agatattgaa gagttaggtc
    54061 aagaaacact tgaaagatac tttaattatg agctatatgg aactgactta cgttatgaga
    54121 atatttgtat agcatcaaat tatttagcta tagtttctta aataaaaagt tttagggcgt
    54181 tctttaaaaa cgccacttcc aataaaaaaa ttgaaagggg aataaatcat gaaaaaatta
    54241 ataattaaga taaaatcatt gtttaaaaag gatttagata gtagctattg gagtactaca
    54301 gaaagtatta ttttaaaatg tggaaacagc aatcaattag aattattgga acaagctaat
    54361 aaattataag gaggtaataa catggtcaat tattctgaat atatgacaca gatttaataa
    54421 atatatgtga ccttttagaa ggtgaaataa taggagaagc aggaacttat aattctttaa
    54481 gtactagact aggaaattta atcggattag attgggcttt aaaaggagaa gaattgagac
    54541 agcaattaat acaaaatatt aataatatga tgtatttaga taatgaaaat aaaattcaat
    54601 ataaagaatt ttaagtaaaa aataattaat ttataggagg aaaataaaat gaataaaata
    54661 atcgtatttt atgatataga agggcatcca ggttttcaag ttcttgtaag aaatgtaaaa
    54721 aaatatacta gacaaaatat aatatttgaa ttggtagagg attatattaa aaataatata
    54781 ggtgaaattg atttaaaaga tgaaaaaata tatagtagtg taagttggaa ggtttggact
    54841 atatcagacg tagcgatata taatacctta tttgatttaa gattagatta caataaatta
    54901 attgaaagcg aagaagagga cactgataat aaggaatgtg taaaagaatt gttttgtatg
    54961 ggtaaaggat gtaaatggat ggaaacagtt ttattattaa gacaaaataa aattgactct
    55021 tctcaatata atattttaac aataaataat tatacatatg tcaaaacatg ggcagagcaa
    55081 tgcaaaaaaa tattattggc taataactat tctaaagtat ttaaatttaa tgagataatg
    55141 aaggattgtg acaataattt aattggggat atatgtttta aatataataa caaggaatat
    55201 aagcataagt ttacatttaa tataaaaaaa gataagttag taaatataga ttgtgaatat
    55261 aaaatacctt ttttaagata tgtttttaat gatatagaaa aatttttaac aagatattca
    55321 gaaaatataa gtaacttaaa ataacaggag ggaaaatatg aataaaacaa ttgaaaacaa
    55381 tttaaatata gagcttaaca atactataga acacttaata ggaataggtt tttgagaaca
    55441 aaaatattat aaaccaactg tatggaaata tgcaaattta gtaatgcaag aattatgcag
    55501 tatggggctt acatatcctt ttgattttgg aagaggtaag ggaaagtatc aaacaaatat
    55561 tgatgaagag acaatttata aaagagctaa agaaaataca aagatacaag aatatagtaa
    55621 aaagtattta aactatattg ctaaaattaa aaaaagtata agtaaataaa attcaacttt
    55681 taatataatt ggaggtaata aaatgaaaaa aataataaat ggatattcaa tatataaaga
    55741 tggaacatgg tgcgaagtaa taaaagaagg aaaatttatc tttgctggtt ctgtaaatga
    55801 aaaactatca tatgaagata tatatatagc tttaactgca aagttagaat ttagattaca
    55861 tggtaagtta ctactttcat atagtctgta tggtacattt caaggggaga aacagtccac
    55921 attagaactt ttagcatatg aaaataattg taatataaat gacattgaag ttaaacaagt
    55981 tgttgaaaat atacaatagt ctgcaaattc aaaaaggaaa caaaatatta aatttaatag
    56041 attacataag ttactatcat tgtcgatata aagttatata ttaattgata atgatagtaa
    56101 tttgataaaa caaacatatt aggggaggta taaaaatgaa aaaaatagct aatttgaata
    56161 aagaggaatt aaaaataata tgggagaaaa atagtcaact aagggaggat gtaaggaaag
    56221 cttgcgaaga caatgacatg tattggatag atgaaatatt atgccatctt aataaatcat
    56281 tagatgattg gtctattgga ttttacaatg ataattatat aaaaatagag gataatcata
    56341 aatttttatt agaacttaaa tctgtatgtg aaaaattttc ttttttatca gaagaagaac
    56401 agagacctct cgagtatgcg atttcattaa ttgataaatt acattgtatg gattttgaaa
    56461 ataaaagatt taatatgttg gaaaataaaa ttaataatat ggtcaaaaaa cttgaagaaa
    56521 aagtaattga agaatttaat aagatgacag agccattgga caaagaatat ttattagaaa
    56581 attttattga attttatgtt gaagcctatc tcaatgataa tgaattttat atagataatg
    56641 agtatgtgtt atacgaaaaa attattaaaa gttatgctta aaagaatgta attggagggt
    56701 tttaaaatgt tagcaaatca gataaaaggt attaaaacat cacttgaaac aagcagctta
    56761 agagaactaa aaggatacat aaaagattta agaaaagcac tatcaggata taaatataag
    56821 aataaaatag atgaaattac agaatatcaa atagataaat tatgttacaa aattgataat
    56881 acagatatat ttgacttgaa atataaagaa atagaaaagt atatagatag tttaattttg
    56941 atacttgatt ttatctttat ggaaatgtaa tcaaaagaat gattttaata taactggagg
    57001 tttaagcatg aatgagtata ttttgataag ttatacaaaa ggcgaaggaa cttgtgacca
    57061 ctgtggaaga actataaaaa atatagctac aataaaaaat aatgttacag gagaagtttt
    57121 tcatgttgga ttaacatgtg tagaaaaaat aatgaaattg aatgttacat tttacaaagc
    57181 attgtcgaga gagataaaaa aacattacaa atgcatggag tactacagta agggtttaga
    57241 tatagagaca aatcttaata aaatagtgaa gaataatact aaatacaaag aaggttctta
    57301 tgcttataaa tctaatgaac aaatgttaga agatgcaatt gcagaagtgg cttggtcttt
    57361 ggctcgtatg atagatagtt gtatgagaat gaataaactt agtaagtctg gattgataga
    57421 tatagatata ttgaacagtt tgtttatcaa gtataaggaa tatatgagta aatttgatga
    57481 aaattacaat aaaaacaaat ataaatataa aagttgttat actatgaaac ctttaaaagt
    57541 aatattagat gaaaatgaag atttaaaaga actatataaa agtatgcaat aaatgtatgt
    57601 aggcgatgaa ttaatgacaa tgaattttat atagatgagg attatatttt atataaaaga
    57661 gtgtcatatc tttaatatag gacatgaagt aataaaagag tttaaaatta tatttttaat
    57721 atgatatatg agttatcaaa tcactatcat tatagctata ttattataat ttaaatgata
    57781 atgatagtga tttgataaaa aggttttaaa gattgtaaaa atacattaag atgattttca
    57841 aacagcttat agaggcttgt acggggtcat aattataaac taggaggata taaaaatgaa
    57901 attcaaaaat atagttatgg gaatgttatt aggtttaatc attgtaggag tttcaggagt
    57961 aacaaaagtt aaggctgctg aattagataa tttttatata gagtccacag atagagttat
    58021 tgaagtttta gaagatgaaa gtatagtttt atatgatact aaagagcaag tttataattt
    58081 ctatccaatt tgtatgggag actggaacta tagctttaaa aataaaaaag atttagatag
    58141 cgcagttgca acttacaaag agctatcaag taatataaat catttaagta atatatacgt
    58201 ggttaataag ttaaatgaca atggaaacat taaagtattt ttaaatgatg gaagttcaat
    58261 tgtttatata aaacaggata ataaatatta cttttatcct tcttgtatgg gtgactggta
    58321 cttaacattg gatagtaaaa tttatttaac taattgtatt aatacttata tagaatatta
    58381 ttattccttg tcaactggag gtggcacaaa tgaataataa tgataagtta tctatattaa
    58441 atcaattgga atacatagaa aatgatattg atgcaagaaa tgaaatgatt gaaaaattta
    58501 aggacttatt ggtcaaatat ccaaagagta gagatataag aagatatata aaacaacaaa
    58561 ctcaattttt aaatggtatt agatatatat atgataattt agatattttg aagaatatga
    58621 taaataatga caagaagtga gaacattact agacataaca atgtctactg aatgtcaaag
    58681 agattcattt agtaaataca aagttattat gtcaattttt aattaaattg taatttaaca
    58741 taaaaatgta gttactattt taaaactcat aataactaca tttaataatt taaaaaggga
    58801 gatggaaaaa gtgaataaat taaaagttaa aggaaaacaa aaatttatga atttagaaat
    58861 accagttata gaaggtggtt ttggtgaaga taaaaaatgt atgacggata agactatatc
    58921 agaaatacat aatcagccta attttaagat aagagagaga ataaaggata atataaaaag
    58981 atttaaagaa gacatagatt atatagattt aaaagttatc cgtgacacgg atgacaattt
    59041 agagctatta caaacacttg gatatagtaa aatgcaaata tcaaaagctg ataatatatt
    59101 tttattatct gaaagaggat attcaaaatt aattaagata atggatactg atttagcttg
    59161 ggaaatacac gataaaataa tggatgaata ttttagtatg agaaaaaagt taaatgaccc
    59221 ttttgaaaac ttatctccag agttaaaagt tataatcaaa ctagaacaaa gacaagcaga
    59281 acttgaatct ggtcaacatg aattagagga aaaagttaat gtcctatatg atttttctac
    59341 tatagactac aatcaacaag aaactattag aagtttggta aatagaatgg taatacaagt
    59401 atatcaatgt ttaaattttt cagcttatga agatgctaaa ttaagaaaaa gagtatactt
    59461 ttcactatgg aaagattata gaaaaaaatt tggtgttaat agctataaga acactgctaa
    59521 aaagaactat gacaatgcaa ttaaattcat agaaaactgg gagcctaatt atgatttaag
    59581 caatgaaata tacatgtttc aaaaagttga aaatatataa gaataatgat tggagattat
    59641 tatgatatgt tatgtcaaag atatacatac tataagccta tatgatgctt ttagatggca
    59701 tacaatgggg ttgtcattca ttattaaaga tggtaaatta aaaggtttta caatgtaact
    59761 aattaattga gaagagaatt atgataacat tcttttctca attaattatc attcctaata
    59821 aataaagttc aaaagatagt gtaaaagata atacattttg ttaatattag taatataaaa
    59881 taaatttaaa aatggagatg aatatattga ttgaaaataa gtttatagaa aaattaaaat
    59941 ataccaaaaa cataccaaac tctttataca aagacttaag acataatttt ttatttcatt
    60001 caaataaaat agaaggaagt acattcacaa gtgaaagttt acaagattta attgagcaca
    60061 acattgtaaa aggtgaccat actttagatg atgttataac aacagtaaat tcaagctatg
    60121 tatttgaaac tgtaatagat acattgggcg aatacttaac tgaaaagtac ataaaggatt
    60181 tacatggaca acttatgtat aattcaaata ttcataatat ggggctatct ggtatataca
    60241 aaaaaatacc aaatagaata ataggaacta atagtgtatt agcccaacca tttgaagtag
    60301 aaccaaagat gttagaactt attgagtggt ataataattt aaaagatatt acattaaaag
    60361 acttagcaga atttcattat agatttgaaa tcattcatcc ttttcaagat ggcaatggaa
    60421 gaataggaag atttatatta cttaaacaaa tacttgagaa taatttgaaa ttgattataa
    60481 tatcgagtga tactgtagat gattatagac aggctttaaa aggttgtcat aatgaatatt
    60541 ataaaccttt agagcaatat tttgaaagtt tagatgacta caaagaaaaa aataaagatt
    60601 tatggagttt ttaacctaat aattaaacaa atgaatggaa gtgaaaacta tgctttacag
    60661 aacggaagat ttaaatactt ttgcaggtat tggaagttct aaaggtgttt attgtcaact
    60721 gccactaccc taaagggtag tagcttgtaa aagctagttg aatagcctaa gtttacacta
    60781 cgttatgtaa gaatatatag ctactttaga atactttaaa cttaaaggaa tattccacat
    60841 attaaaatac tctttttaca aatttataat taaatcataa tctaactttt tatttgaaaa
    60901 actttcaatt aaccttgctt tatcattagc attctttaaa atgtagttat ttaaccaata
    60961 aactatcgca tcacccctat aatggagtcc aatttggact ccattttcat aacaccaaaa
    61021 aggatgctca tcttctgtat tgtcaatcca aatatgaaat tcgtttctaa tttcatattt
    61081 ttttaaacta atttcattag gaacatatat ataatcagga ctatagttgc caccaaaatt
    61141 tacttttata accataatta tagtctcctt tttaaataat tataacatat tttataaaaa
    61201 actggtaatt acacttgtag tttaattcca aaatatagca ttctacaagt gtatctaaat
    61261 ttattaaagc aaaaatataa aaaaataaag gatactatat atttaaatgc aataattttt
    61321 gtatttaaat ataaaaactc atatatttat ctataatatt atatctatta aattataaat
    61381 aactaaagcc aatatacata agaataacca cccaataaag ctttcattct tactttcaaa
    61441 catatttaaa aattttaatg catctttaaa tatcaaaata gatagtttta taattacaaa
    61501 aactgctaat agaataaaca atgttttaag catatatttc tccttaataa ataatttaaa
    61561 atcttattta atataaattt tgtttaaatt aatctgttat ttataaataa attatatcaa
    61621 agtttgttgc actttagaaa tatatgtaaa taaaaactat tagtcaactg gttaatatgg
    61681 tatgacaaca ataatctcat gtagtatact taatttagag gtgagtatat gacttggaat
    61741 gatttaaaaa acaatgatat aacatatata gttgaatata ttaataataa gttaaataat
    61801 agtaagagtt tgactaaagt tgctataggg cttggtgtgt ctgaaagtag cataagaaaa
    61861 taccttacta aaagaggtta taaacgtata aatgatgagt atgtgtttat tggtgacaat
    61921 agtatgacaa aagtagtaaa caatagacaa cagagtaata atgataatac atttaatatg
    61981 acaatagaag atgacaacaa tcacaacata gtaattgaca accaatttaa aaataatata
    62041 gtctctttag caaaagacta tgataaaata caagatgtat taaattggtt tgagaataaa
    62101 gaagatacaa atgtaataga agttgtacag gatggtatta agattgattt acattcaaaa
    62161 gatgctataa ggactacagt taggttgaat aaagatgcat ggaatttatt tgatgagttt
    62221 tgtggaaaat tcagagagtt taataagtct gatttaatga gtatggcttt attagaatac
    62281 ataaagaagt atgataaata aaaatgacaa taaaatcgta gttttaattt aaataacctc
    62341 attattttga ggttattttt tttattttct atttaaaacg gagaaggtaa atgttataat
    62401 atatatataa ttaaaaaaag gatgtttgaa tttatgaatt ataatttaaa aaatgaaaat
    62461 gtattatcag agctattaca aaatgaggat gaaaaaacaa ggattggtat actcaaagtt
    62521 tataaagtgt taatattttg gtttgctaaa gaaggaaagc ttgttgagga agaaaatgac
    62581 tatattgttg agatagtagg taatgataaa gaagaaattg tgagagatat gagccagaat
    62641 aaaacataca atgccataat ggctataatt tttaaaatta ataagatatt taaaacttta
    62701 aattacgata ttcacttttc taaaccaaaa aatttagtcg ctgaatttga tttatcaaaa
    62761 tatctaacac aagatgaagt acttcaggta tgtagtaagc taactaatgc acaagataag
    62821 tttataattt atgcagcttt ttgtggtctt attggtaaag atgctgaaga cctaaggggt
    62881 ataaaaataa aagacataga tttagaaaaa agattaataa agttaaagga tacagaattt
    62941 aagctggaca agttcttatt tgattttaca aaagatacat tagaacaaaa agagtataaa
    63001 caatatttgg agcaagacag caatgctgct gaattttatc tatttaattc taattctgaa
    63061 tatttaatta aaactaaacc agatgctaga tatggaaatg gattaaaaga gatgggatac
    63121 tcaacaattc gcaaaagatt acttattatt aatgattatt tagataataa agtacagttg
    63181 aatttcacca atttaaaaat ttctggtatt atgcatcaaa tgcatctaaa tgaaaaaaaa
    63241 tatactcaat ctggatattg gactaacaaa agcttgaaag agtttaagga ggataacaat
    63301 ttaaacttca aaatacaaga agttctatct atttataagc aaaaatatat taaggagtaa
    63361 ccttatgagt aaagatgtta aagaaaacat aagtaatgaa caaaaagatt taaataaaat
    63421 aaatttaatc tatcatatta tatatgacaa tgatagattt aaaatgataa caagaaaata
    63481 ttttgaaatt tttagattaa agggtatgtc tgctgaagaa ataagtttaa aatttgaaga
    63541 atttctatat tctcttattt cagataagtt taaaaagata aatacagtaa atgatgttaa
    63601 aaaattaaga aatcaagaga actcatcatc attaaaactt aagaagagat ggtctgatat
    63661 gactttagat tctctaagtg aagatactaa atcttcaaga aaaaaaagca gtaaatacct
    63721 aaaaaaagaa ttagaaattg ttgaaagtga atatttaaaa aatggaagaa tcattagtgc
    63781 tggagtaaaa aatccaaaag aattaaagga gcaaagaaaa gattttttag atgagtggaa
    63841 gtcaagctct agttataaca tatgtgatta cccatattta caagagttaa gtactagcaa
    63901 aataaattcc gcatttatac atgatatact aatgtgtata acagaagtac taaaagatga
    63961 ttatgatttt gatattaata aaattgcaat caaaactcca ccacatattt cacctggaat
    64021 ttttttacct ttcacaaaag gtaaaagata ctcgcacttg aatgttaaaa aaaatgaaaa
    64081 tgaatacatg tcagacgact atagtgctaa gaatctttca ggtaaacaaa tgaatttttt
    64141 ctataagttt tcaattgggc aagatgaaaa tattgaaaat cttaaactag agctaataaa
    64201 tcctgataca gaaaaaaggg ctttgccaaa aatatattta gataaacaag accttgaaat
    64261 tattagattt gtttatactt atagccacat gaactctttt tcattttatt taggcgattt
    64321 agtaaagtac ttaggacttt cagatggaaa aaagaactat attaatatca aaaatagact
    64381 cttaaaatta ccttattata ctttttattc gaatcaaact aatgataaag gcgaattaaa
    64441 gtatgagatt agttttaatt tgttttctag tacaacaata gtaaatgatg aaaacaataa
    64501 taatagagag ttggttaaaa tcacaaaatc ttttattgaa gaagtcgaaa gagttaatac
    64561 agatataatg tatagaaatg aacttgaaaa attacaatta acacagtcaa ttaatctagc
    64621 ttatttctta gagggtagaa ggtcttttct tatatcttgt ggtgaggata taaaaaataa
    64681 aagttttaga tatgacatag aagatttaaa atttgatgtt catcttaata aaagtaaaac
    64741 agtaaagcaa aatatggagt atttagaact aggttttaat gagataaaag aaaatcaatt
    64801 tataattaaa gactataaaa gaggaaattc ttattttgat gtttatttct atgaagattt
    64861 tgaaaaaaga aaacgtctta taaataatac tatactttct ttacctgact acatgataga
    64921 aaataaatct taaaaatggc catatattct ccgtacttaa caaaaaatgt cgaatttatt
    64981 ttaaataaac ttaataaaaa aagtaaaatg accgtatatt ctccgtactt aacaaaaaat
    65041 agatttttca aaaaaactaa atgaccgtat atcttccgta cttaacaaaa aatagacctt
    65101 tttttaatta tttccttgtc gaaaaatttt ttttgtaaca tattttttac ttttcttagt
    65161 aatttcaaat ctcacgtctt ctgaaatcac tatgtctgag ctaaatgacc gtatcagctc
    65221 cgtacttaac agaagacgtt gaaaccactc tatttgagct aaatgaccgt atcagctccg
    65281 tacttaacaa atatagacat aaatttattt tttgggtaag ataaatttga gtaaaaggaa
    65341 tcatcaccaa aatggctata tcagctccgt acttctgaaa ttattgattt ttcaaaggag
    65401 aaaattatga gtcttaaata taaagataaa gaatttgtac ttgtagaaaa aaagaatata
    65461 aatgaattag ataactctaa tataaaatat attgacctaa aggacaagca gtattatgtg
    65521 gttacacaag gcaaaaaacc taaaagattt aacctagaag atattaaaaa tataaaaaaa
    65581 gatttagata atggcataag tttaagaaag tgtgcaacaa aatggaactg tagcactagg
    65641 actatacagg atataaaaaa taatatttat taatatacag aggtatttat ttactttata
    65701 cctcattaag attataataa tcatatatag agaaaggagg attataaaat gcaagaatta
    65761 aaaccagttg ctagaatatc aaagtcaaaa ctaaaagata tattagatgt ttttaaaact
    65821 tctataagaa aagtagaaaa aatagattat tctgcttgta attcttgtgc aataaaaaat
    65881 aatccttctt ttgatagtca taaattaaat gaattttgtt caaattgtcc taaattaaaa
    65941 actatatcag agttttctta tgttacagaa aaggaaacag aactaggtac aatagaaatt
    66001 gtaactaatg aaagtaaatg tatggcttta agtaaaagtg ctataaaaca gtatttagca
    66061 tatcatttta tagtatctaa taaaaattat gttagaaaag cagtatcctt ccaaaatata
    66121 gccgatatat gcaatgtttc cattgttaca gccagacata accataatgt attactacga
    66181 gttggattag tttattctac agaaataaaa agaggtaaat ttgatatagt catagatgaa
    66241 gagtacaaaa accatcttaa aaaagaagat ggtggaaaag gttacttaac tatgtcatta
    66301 gatactttaa atcacttatt aagttttgaa aatgtaaatg agttaaaact tgaaattaaa
    66361 aaattaattt gggctgatgc taaaggaagt aagttaggta aaagaatacg atttaaaaaa
    66421 gacaatctta tttctatact tccagaatac ataaagaaat caaacaagcg tacagaagcc
    66481 tttttaagaa gttctaaaac tttatttgat gtaagtaatg acacaataga tacaaagcgt
    66541 tacgagacga aagaaactat ctcagagaaa tatacagaac caattagaat acaaatatcg
    66601 aaattctttg ataatactgg ttgctctttg agttctaatt atagtaaagt gttatctcag
    66661 ataaatttag atagatataa tttatttgaa gaagatttaa gaagtaaatt agacatggaa
    66721 aaagatatta ttataaatga cttagttgga ttgtcggtgc agtatggagt tactagagtt
    66781 ttagcagcat tagattatat gtttaataaa tatacagttg atgaagaaga tataaattat
    66841 aatgaaatac gtaatcctgg tgcatttata agaacattga ttgttcagag cataaataaa
    66901 aatggaagtt tagcaatagc aatttaatat atggagtagt tcaaaaaaaa tgattttggc
    66961 tactcctatt agtgtttgta ttcaaattat aaaatactta aatagagcta taaagagcgt
    67021 gaaatctcta ttagctctat ttttttatgc ctattttaat gaaattatat acacaataga
    67081 aagtttttta tacatattac taatttaatt ttccaataat aatatatatt tattcaattt
    67141 attaagaaac ttattaaaaa aaaataataa tacatcaaca atatattatt attaaaattt
    67201 atattttatt gtattcattg taattacttt gatgtatagt tttttcttat tttctaatta
    67261 ttgaatttaa tttttgtatt aattgctatt aataatttct gtattaaact aatgagtaag
    67321 ttataactat gaaacttatc ttacaaagtt aataaatata tattgcatta agtaataata
    67381 taattacatt caaagcgtta tataaatata tattatttaa tacttcatat cttatatatg
    67441 ttatcatttt tatttacctt atttttacaa attattccat aagtatatta taattatctt
    67501 gagggaaata atcatataga gggagaaatt agaaaatgga tagatttgaa gtattaaaat
    67561 atctaaaatt taatgtttgg cattcagaat ggccattaga gcttctcaaa ggggctataa
    67621 caaaggataa tgctactggt aatttattat tacaattaaa aatgtgtaat atatctgata
    67681 aggagataaa gtctgcatat tttacaatag aatgttttaa tgatgcagga gatttaatag
    67741 ataataattt aaagtatgtt tatcaagata taaacattaa accacatgaa tgtcttggtg
    67801 atgatattgc tataaaaata aatgataata aaacaagagc aataaatatt atattagaac
    67861 aagtagtatt ttcagatgat aaagttcata aatatgaaag agaagaaggt ttaattatac
    67921 ctaaattaga taaaatagaa ttgttaaata tagaattatt agaagaactt aaaaaagatt
    67981 atgaagaata tgattttcaa tatgaaatta agtacatgcc taatataata gaaaaaaaag
    68041 catggagctg ttgttgtgga aaaataaata aattagaaaa agataagtgt gataattgta
    68101 agagaagtaa aatactgcaa ataaaaaaga taaataaaaa ttatttagaa tctatttata
    68161 aaaaaagcct aaaaaagaaa aaagaaatag aaataaagct tgaaaaagat aatagacata
    68221 aattaaagaa cataaataaa aataatagaa gaacatcaat attattaata agtatattat
    68281 tgatttttat tatttttatt ggtaaaaata ttattaataa gcatgctttt ttagatgaaa
    68341 taaagtataa aaatgaatat agtaaaaaaa tatctgtaga aaaagataat gtatatatgt
    68401 taaaaaataa tggaaaagta acacatagaa aagtgaatgg tgaaagtagt tctatacctg
    68461 gttgggaaaa tataatatct atagcatcat atgataactc aataatagga ataaaaaaag
    68521 atggtacagt tgtttctaaa ggacttggaa attcagattc agacaatatt agtaaatgga
    68581 gagatataat agatatagaa tgtgatagtg gaacttttat aggattaaaa aaggatggta
    68641 ctgtcgttgc tactggaaat aatgattatg gtcaatgcaa tgtgaataat tggtttgatg
    68701 tagtaagtat agaaataaca aaatcaacca ttgagtatat gaaaaataca acagtaatag
    68761 gcataaaaaa agatggaaaa gtattactta caggatatga tagtctgaat catagaaaag
    68821 aaacagacaa atggagagat atatcaaaga taaaatgtgg gtcttatggc atatttggaa
    68881 taaagaataa tggcacagta gttgtatcag gtagtgctta tgatattaag aattggaaag
    68941 atatagtgga tatacatatt ggattatgtg gaatagtggg tattaaaaga gatgggacaa
    69001 taataagcac agatgaaaag tttaatgaaa tcattaaaaa ttggaaggat attatttctg
    69061 ctgaaataat agataatcat gaaggaattt ctattgtagg aattaattca aatggagata
    69121 ttttaacatc tagtagaggt cagaatttgg ataatataaa aagattagaa gatgtagaat
    69181 atataaattt tgatgactcg attgataatt ttttagtagg tataagaaaa gataatgtaa
    69241 tttttataag aggaataaac aatactataa atgaagaata caacgaagaa atataaaaat
    69301 aaatatttta atttggagga atagaaaata tgaaaaatta catagcaaga tgtttatcaa
    69361 atatgggagt attgttggca ggtatacaag tattagcggg tataataatc atgtgtcaaa
    69421 aagtagaagt tccatatgat tatttgtcag atacttatga acaatatagt cctattatga
    69481 ttggtattgg tgctggggtt atagtaagtg gaatcgttat atgtttagtt ttaaagggat
    69541 tagcagagtt agttgaaaat tcagctgacc aaacacaata tcaaaaagaa atattaatgt
    69601 tgttgaaaaa taataatgta aatgaaaaaa ttaatgaggt taaaattgaa caacaattta
    69661 caaatgagga attaccaaaa ttataaaatg ttttaaatta tatactaata ctcagtacta
    69721 attaaagtac tgagtatttt tatgtaacag taaattatat taatataaaa atatattcta
    69781 catacataaa atgataaaat aaccatttat aatacattaa attacattaa atcaaaaaag
    69841 tatatgtcga aaacatataa tattaaataa atatatactt taaggagtga tttaatgatt
    69901 ataactattt gcgaaaatga tgatatgcaa agagatatca tctatgattt tgttaagata
    69961 tttttaacta aaatcaaggt aaaacattat gacattttaa tgtttaagaa tggggaagaa
    70021 ctgttagaag aatatccaaa caaagtggat ttaattttaa tggatataaa tttaggaggt
    70081 agaaatggat ttgatatttc aaaagatatt agaaaatttg ataaagatgc aagaataatt
    70141 tttgtaactg tatcagagaa atatgctatc aaatgttatg agataaatgc atttagatat
    70201 atgataaaac cgatatatta taaagaattt gaaaaatata tgcttgactt tgcaattgat
    70261 tggagaaaaa gagaagctat tattactata aaaagaaaaa aacaaataat aaaaatagag
    70321 gaacataata ttttatttgt tgaaatagtg aatagaaaat taagagttgt tactaaaaat
    70381 gaagaacata taataaatga taaaatggat atgtttgaag aaaagttaaa agaggatata
    70441 ttttttagat gccataagag ctatcttata aatttacttg aggtaactaa tttcaattat
    70501 aatttcgttt atttaacaag tgagaaaata aaaattaaag tagctcctaa aaagaccaaa
    70561 aaacttaaag agaaattagc aaattttttt agggaaataa tatggtaaat taaagaaaaa
    70621 tttctataat cgaatatatg ttcttgataa aagtatgtgt acaagatata atattattag
    70681 taggaaaata ggaaaaatat taatattttt gaacattttg acattaattg tgattttagt
    70741 gttgaatttc tgttaaaaaa atcttattat ttaatcatag aaatctactg ttatagagta
    70801 gaagattgtt aattataaat gattttaaat tatctgagat aaaatttttt gtttctgaaa
    70861 agaacgaatg ttcgaatgag gtgtagagtg agaggatgtt tattaaaaga tattagatta
    70921 tcttacttaa ttcataatgc atatatgggg gttctataat aatgaaaaaa caagtcacaa
    70981 aagaaatact taaaatgcta gaaaaaatgg atttatctta tttactcata attaaaagtt
    71041 ttgtatctaa attaaaatag cccttattgg gctatttact aagtttatca attactttct
    71101 gtatgacttc tctttcatca tcagtaagtt cagcaaatgt taagaatact ttttttaaaa
    71161 attcatcatt acttgagaat gttttaccca tcattgctgc taattcaata tcatctatat
    71221 ttttaaacat ttctccattt ccatttcgta accagtcctc atttacatta aaaacttcac
    71281 atatctgaaa tataaatcta tctgttagct ttctttctcc tttttcaagt ctagaaatag
    71341 ttgcatcact tactaataat ttttctgcaa attctttttg tgagagacct aatttaattc
    71401 ttaattcctt taaattctca ttcatattta cacctcctat tatatagtat attaataaaa
    71461 attgacaatg tcaataaaat aggaatgata aattgcaaaa agcaaaaaaa ataattgaca
    71521 aatacagaat gtggtactac aatgtaaata tacaaaacgc aattaataat ttgacaaacg
    71581 caaataaata aggaggtaga ttatttgtat aataataatg ataaaaaatt aataaattta
    71641 tttgaatcat ttaaaaaaat agactataca gaacaaagtt tagtgtattt tttatcttta
    71701 agctttatag taaacagaaa atgtcataaa gatgcaaatt tgtatagtag gagaggtgaa
    71761 tgtaattatg aataatttaa atatcaaagg aaaacaaaaa tttatggatt tagaaatacc
    71821 tattatagaa ggaggttttg gagagggtaa aaaatgtata acagataaaa ctatatcaga
    71881 aatacacaat atggagataa aacatgttag agaattaata aataggaaca taagaagatt
    71941 tataaatgct attgatttta tagatttaaa agtggtcgtt gataacgact acaatttaga
    72001 agtattggag ttgcttagtt atagaaaaat gcaaatatca aaagcaaata atatttatct
    72061 tttatctgaa agaggatatt caaaattaat taagataatg gatactgatt tagcttggga
    72121 gatacacgat aaaataatgg atgaatactt caatatgaga gaacaactca tagatagtta
    72181 tatgatacaa gattctattg aaagagctaa aagatggata gaagaagaaa aagaaagatt
    72241 attacttaaa gaaacaattc aagttcaaca acctaaagta gaatatcatg ataaagtatt
    72301 aaatcctgac aaattagtta cgacaacaga tattgcgaaa gatttaggaa tgacagcaca
    72361 agcattaaat aaattactac atttaaacag aattatatat ccagaaagaa taaatggtcg
    72421 aataaaatgt tggaaaccat ataaaaatta tttatggttg atacctgaat atgcagattt
    72481 taaaataaca gaacatgata cgcagcttaa atggactgag ataggtagaa aatggttaat
    72541 agaatttgta gagaacatta aaaaaataag ttaaatcaag aagttaaaat gtaccttgaa
    72601 aagtagatat ttaaaaatat aaaagatagg tgattaaatg ataaaacaaa tactaaataa
    72661 aagaggtagt ctattaaatc tagcaagtga gtatttgaca gaacaagact acctcaagtt
    72721 tcaaggatgg tatacacata gatatagtaa gctggatgct tatgatgctt ctataaatga
    72781 atatttaaat tataaaaacg acaaaaaatt aataacttat gtatttaatt taaaatttca
    72841 agaagctctc atagaaatat aatattacta aacatggtga ttgaaatgaa ttataattat
    72901 gtaactaaaa ttgacttgaa atatggaaat acacaatttg ggtttgagaa atatagcgat
    72961 gctttagata agtacagaga acttaaagaa gaatttaagt atgttccatg tattataaaa
    73021 cttcattcat atgtagcgca ccaagattta tatgtgaaag aaattaaagc tgatggtttt
    73081 gaaaagaaat atttagatat taaaaattta ataaaattgt ttaaagaaca taaacaaaat
    73141 atatcagatg aactaagtct ttgtgataag aaaaaggaag aagtattaca tgtaatagaa
    73201 gaaatgaatt tagacacagc ttctttaaat gataagattg ataccattaa agaattaaaa
    73261 aacactttag ttaagagaag gttggttaaa tatgaactaa ataaatactt tgccttttgt
    73321 aatacattat atgaaattga agaagagtta aaaaaatatg aatctaaata taaacaaaaa
    73381 gaaaatcaag gaaaaagcag attcaaaaaa aataaaaaat aggtgattag atggaagaat
    73441 atcattttaa cttaataaaa aagctttgtg ctgaaagaaa aatacacaaa ggttggttta
    73501 gaaatattga aaaatgtggt tgtggaggta aatgtaataa gaaaaattgt aagatgttag
    73561 aaaaattata aggagataca tatggaaaat caaattgaat taacagaaaa agatatagag
    73621 aacactttaa gtgctttaaa tattattaaa tatatttgta taaaatctaa acattgcaaa
    73681 aattgtcctc ttggatatat gaaaaatctt gatgaacata attgtatgct atatgaatta
    73741 atagacaagg gtttacttcc atgtgattgg tctgtaaaaa aagttacaag attgttagat
    73801 acgacagaag aataaaacaa aggagctggt taagtggagt tagatttcag aatttggaat
    73861 ggatttagaa tgatacatgg agaagaatta agattactaa cagaagagat gtttataaaa
    73921 agtggatatt ctgaacaata ctataacgat atttgtatac caagagtaga agatttgaaa
    73981 ataatgcaat acatagggct aaaaaattgt tttgaaaaaa agatatttca atatgatatt
    74041 gttaaattta tctttgatgg tgtttggaaa gtatgtttag taaatttatc tttgccaaaa
    74101 ggagcaacag ttacatttga aagcataggt ggaaaatata cttttaattt aagtgaaata
    74161 ttttcaaaag ataggaaatt agaaatcata ggtaattttt atgaaaataa agatgtaatc
    74221 aaaaaatata atcttagtat tgaataggag aagaaaatgg atgaaatgat aattagggtg
    74281 aaaatgggag aaccattacc aacactagga aagataatca aatgtactac atgtttaact
    74341 aatgaagaga aatgttttaa ggttaataag atatgtaaat taagttggat taacgatagg
    74401 aatagtgaag ggttaattgt agaatgcaaa ggtaaatata ttaaatatta ataagaataa
    74461 tgaagttaaa atcaagattt taatagaaaa ggagtgatta aaaatgaaga aaaatccaat
    74521 aaaaattaaa aaatgtgact gtggtggaga tatatatgaa cttaatacac aattaggcac
    74581 tatgtatcaa tgtaaaagtt gtggcgagat gtatagatag tgctgaaata ttagctaaag
    74641 aagatgaggt gaagtcaatg agtaaaaaag ataagttatc tatttttata agtttaatat
    74701 taagacataa accagaaact attggaatta aattagatga ctatggatat gcagatgtta
    74761 atgaattgat tgaaaaaata aataatactg gtagaaatat taatatagaa atccttgagc
    74821 aaatagttaa agaagataat aagcagaggt acagttttaa tgatgataga agcaagatta
    74881 gagcaaatca aggacattca attaatgttg aggttgaatt gaaagaatta gaaccgccaa
    74941 aatgtcttta tcatggaacg gctactagat ttttagagaa tattaaaaag gaaggtatta
    75001 ttagacaaag tagactgtat gtacacttat caaatgatat agatacggct gttaaggttg
    75061 ggaaaaggca tggtgtacct gtagttttaa aaattaatac aggaaaaatg tatgaaaatg
    75121 gttataaatt ttatttatct gaaaataata tttggttatg taagtatata ccatttgaat
    75181 atgtagagaa aataagtaga gtagttaaat aaaattatag aaaataggag gttattgaat
    75241 ggatttcaat tttagaaaga tttgtgaaaa agatgaactt actgatgatt ttattataaa
    75301 aatttgccag ttgtcacatg aggagtttgg aggcactatt tatgtaccat gtaatattca
    75361 tccatgtggg tgggatgagt tggaagttcc gaatccaaag gatttagatt cagataaatt
    75421 aatggaagaa tttttattag aatataaatt tgaatgtgaa aatatttcga gcaatattta
    75481 tgatagtgac catccattta taggcaaaac attgagagag attcttatga tagtggattg
    75541 tagattcata attggtgtta tataaggagg gtattaaata tggaaaatat agaattaaca
    75601 cctagagatt attttgagga tataaaaaat aaaaagcaaa aaaatacaaa tgaaaatctt
    75661 gatatagtat atgaaaactc tttgattctt ttaaacaaat atattgtaac tggtcaaaaa
    75721 aagggtgcta agaaattaat gtttttactt gaatgtattg aaaaagaaag aaatgtaata
    75781 gagctgggaa tagatacatt tgtgtataaa gaagatatag aaaaatatat tgatgatata
    75841 tctaaaaata cagtaaaaat aatagaactt gaaaattatg aaagagaaat accagatgaa
    75901 atagtagaaa taatagaaaa aacaaaagat atatttgata aaatgtatgt tttgtttact
    75961 gattatacag gtaaaattac aaaggagata agcaaagaga gaagagataa agacccaata
    76021 ttatttggtg ttttttcaga tgagaaaagc agagctattg tagatagatt ttattatctt
    76081 ggagattggg aggatgagta ttgtgattta acattagaga aaatgataaa tgattataag
    76141 aaaaaaggaa gacaagacat attaaaacat ataattacac ctaaatcatt agatgagtta
    76201 aaaaaagaat tagattcttt agaaaaaaat aatgatacat ataagcaagt tgccaatata
    76261 aagccattac ctactacaaa aaactctagt gtagaaccac ctaaattaaa tatatttaaa
    76321 aaaataaaaa atagagtgtt taaaaatgaa aagtaatgtt gatttaacag agaataggga
    76381 ttttagagat gtaagaggaa gaggcatcaa tattttaggt atcgaagatg cgccaaatag
    76441 aatgttgagt tttagtgatt ttggaattgg acgtctttat ggtaaaaatt tagaatttga
    76501 atttaataaa aatgaagtat ttttaactgg aaatgctaat gacagagaag aaaaaaagtt
    76561 tcttatcctt tgtgaagaaa ataaggtttg tgttagatgt ggtgatgaat taagagaggg
    76621 gtgggataaa gtaagcatag aactatgtaa tgaatgttat gaaatgttag atgttgagta
    76681 taaaaataaa aagataatta gagaattatt ttttagagat agagatgtaa atatagagtt
    76741 actttaaaaa atatataaga aaaataagta gaggaggaat ttatatgaaa gatgtttgat
    76801 tatatagctg ggatgatgag tgctttggaa gtgatgaata cgaaagtaga gaggaagcta
    76861 ttcaaggagc taataaaact agtattttta gtgatgacaa cagtatgaca agaataatga
    76921 gaatataaag gagtgatgtt tattaaaagt tttagatgta tattcagaca taagttttca
    76981 aggataaaag attgtcaatt aattacaaca aggagtcagc aatatacctg taaaatttgg
    77041 atgtgtaaat attgtggaca aattgtagct ggttcaactc ctgtttatca tcctaatatg
    77101 aaagtcaatg agtattgtgt aaagtgtgag ttttcggcat gtgcaaataa ttatgaggga
    77161 tattgtaact ctaaagataa agaaattgaa tttaagtcta tttgcttaaa aggtaataaa
    77221 gagattttgc agtgtaaaaa ttttgatttg gaataaaatt tagcttaagt aggagaattt
    77281 tagaatatga aaataaatgt acatgttaaa actaataaaa ttggttcaga ctgtgagctg
    77341 gagtttgaaa tagatgatga tgatttaaaa gatatgtctg atgaagagag agagggttat
    77401 atagataaaa ttgcatggga ttatgttttt gaaaatttta tagattggaa ttggtatgag
    77461 gttgaagatt aagttaaaac atttgtttta atgtgatttt acctagtaga tacaaggagg
    77521 tttttaatga aagagctttt agaagtaaaa aaaatatttg atagtttagc atctacatct
    77581 agtagaaaag aaaaagagag aatattagaa aaatataaaa ataatagaat gtttgttgaa
    77641 tgtttacaat ttctactaga ttcaaatatt ctcactggaa tatctaagaa taaaatatgt
    77701 aagaatttaa ataatactgg tcataatgaa ttagagaaca tatatgatat gcttgactat
    77761 ttaattaaga ataatactgg cagaaatata gatgtaaaaa caatacaagt atttgccagt
    77821 aaagatgaaa aattaaaaga ttttattttt aatttagcta caaaatcaat taaattagga
    77881 ataacatata agactgtaga caaaataatg ccaggtttaa ttatctaata aattttgtat
    77941 tttttacaaa aaactattga gaacatagag gttatatgat ataataaaaa tatattgaga
    78001 acagagaagt taaaaagggg ggcaatgaga tgactgacaa aataataata gcaagtgtag
    78061 atggagagga aatgatattt agaactgaaa tgaaaaagtt agaaaaagga gatagtctta
    78121 tagtttctgt aaatggtgta aaaagattag ctctattcat taggtatata agtacttgtg
    78181 aagaatatat agaagatata gcatttcaaa aagtagatga aatattatat gaaaacacaa
    78241 ttgccaaaga aaaagaaaga gttaaaaagg tatttaaaga taagtgtatg aaaaattata
    78301 aacctattgt taatacagaa atagactctt atttgagaga atgtggtttc aattgtgatg
    78361 gtagacaatt tttcaaaagt atagacaata cacaaatttt aattagcagg gattgtgata
    78421 aagatgtttt gactgtatcg aaaggtttga caataactaa aacagatata acaaataaaa
    78481 atttaattac agttattaaa ataataaata atagtctatt aatagttagg taatagaaat
    78541 aaatattgaa cttttaataa gaaaaaagat tttaaataaa aggagtgatt taatgaagaa
    78601 tgttattaaa agagatggga ggatagttcc ctttgatgaa aagaaaataa taaatgcaat
    78661 agaaaaaagc tttttatcat taaggaacaa aggaaaaagt gaagtatttg atagagaatt
    78721 agcaacacta atagctaaaa aaataaaaaa taaaccatgt gaaaaaatgc atatagaaga
    78781 tatacaagat ataatcgaat ttagcttaat ggctagtaaa aagaaagatg tcgctaaaga
    78841 atatataaca tacagagaga aaagaaatat agcaagagga agaaaaactt ataatgatta
    78901 tatgtctatt gtaaatactg aaagtaatga tataactaaa gaaaatgcaa atatgaacgc
    78961 agatactcca gcaggtatga tgatgaagtt tgcaagtgaa gctactaaaa cttttactga
    79021 ccatgtactt ttatctgaag aagcaaaaga agcaatgatg gataattata tacatataca
    79081 tgataaagac tattatccta cgaaatcatt gacttgtatc caacatcctt tagataaaat
    79141 ttttaataat ggttttagag cgggacatgg ctctagtaga cctactaaaa gaatagagac
    79201 ttcaagtata ttaggatgta ttagtttaga gactgtacaa aatgaaatgc atggaggaca
    79261 ggcgatacca gcttttgatt tttataatgc tccgagtgta agaaaaacat ttatagaaga
    79321 aataaaaaat atagaaataa aggatgatat tgatttatct catttataca atatagaaat
    79381 agaggattat ataattaagg acttaaaaga attagacagc gaaaaaagaa atgttcaaca
    79441 tgctataaac agaactgtat ggagagttca tcaatcaatg gagtcattta tacataatat
    79501 gaacacaatt cattcaaggg gtggaaatca ggtcgtgttt agttctataa attatggtac
    79561 agatacttca gcagaaggta gatgtataat aagagaaata ctaaaatcta cttatgaagg
    79621 agtcggaaat ggagaaacac caattttccc aattcaaatc tggaaagtga aagaaggtgt
    79681 gaactataat cctactgata gaaattatga cttatatgaa ttagcatgta aagtatcagc
    79741 taaaagattt tttcctaatt ttttaaattt agatgcaacc ttcaataagc atgtaaaatg
    79801 gaaaatgtgc gacccaaaca gatatattta tgaagtagca acaatggggt gtcgtacaag
    79861 ggtatttgaa gatttgcatg gagaaaaaac aagtatagga agaggaaatc tatcttttac
    79921 gactcttaat ttagtaaaaa tggcattaga ggctaaagaa aaatatccaa ataatgaagc
    79981 taatagagtt gcagagttta tttttcttct tgataaatat tcagatgtag tagcaagaca
    80041 attatatgat agatacttat ttcaatgtac tgctaaagca aaacagtttc cactattaat
    80101 gagtgggtta tggaaaaact cagaaaattt aaatccaaat gatgatgtaa gcgaagtttt
    80161 aaagcatgga acacttgggg taggatttat aggtctagca gaatgtttaa tagctcttat
    80221 aggtgagcat catggtgaaa gcgaaaaggc acaaaaatta ggtttagaaa tcatatcact
    80281 tctaaagtct aaagtagaaa aatataaaaa gaaatataac ctcaattatt ctgtattagg
    80341 cacacccgca gaaggtcttt ctggaaaatt tactcaaaaa gataaaaaaa tatttggaga
    80401 aataaaaaat attacagaca aagattatta tactaatagt tctcatgttc cagtttggta
    80461 taactgcaat gttgaacata aagcgaaaat agaagcacct taccatgaat tagaaggtgg
    80521 tggccacata ttttatgttg aattagatgg ggatgcaaca cataatccta aagctataat
    80581 gaaagttgtg gatttaatga aaaagtacaa tatgggatat ggttctgtaa atcatacaag
    80641 aagtagatgt atgaattgtg gttttgaaaa cgcagattca gagttaaaag aatgtcctaa
    80701 atgtggcagc aatagtataa atattatcca aagaattaca ggttatttag ttggaactac
    80761 ggatagctgg aataatgcaa aattagctga gttaaaggat agagtcacac atggataaag
    80821 actatattta tattttagat ataaaacatg acacaattgt tgatggagaa gggtttagaa
    80881 catctattta ttgttctgga tgtaatcata tgtgtagagg ctgccacaat cctcaatctt
    80941 gggatataaa aaatggaact ttaactaaag tattagatat ttataaggaa atcatttcaa
    81001 ataaattttc agatgtaaca ttttcaggag gagacccact tctacaactg caaggatttg
    81061 ttcatttagc taaattaatt aaaaagaata caaacaaaac aatatggtgt tatacaggtt
    81121 ttaagtttga ggaactcata acagacaatg aaaagctaga attgttaaaa ttaatagatg
    81181 ttcttgtgga tggaaaattt gaagaagata aaaaatcttt agagttgatt tttaaaggtt
    81241 cgtcaaatca aaggataatt gatgttcaaa aaacattgta tgaaaaacaa atagtattat
    81301 atgaataaga gaggcttagc cctctctttg gaggtaaatg ttatgaccaa agaagaattt
    81361 attactaaat ttcttaaaga gcagcagaga agaataaagg ttttatatta tgaagttgaa
    81421 aatttcaaga tggatagaag gtttaatgag aggaaaaagt ctaggagaca ttaaaatgtt
    81481 tcttttaata taaggaggaa gtagctatat gtattttgaa acggacatta taaaaaataa
    81541 gaatactgta aataaaataa taaaatattg tggtggaaat actgaaaaat tgaaagattg
    81601 gctaaaagaa atagaaaata aaagtatcag tagtttagaa gaattaaaag aatatccatt
    81661 aaaaagaaat tataaatttg tattttccaa agaagaagct cctaatttag ataagcttat
    81721 aaaaatgaaa ctaagagata attgtttaga aaaatgtgag atttgtagtt tagaaaatct
    81781 cttaagtatg ctaaatgatg agattttaga tttaaataaa gaagatatct cttatgaaga
    81841 taatattgaa tggattgaaa attgtaaaga agaactaatt aaattaaagg taggaagtat
    81901 ttgcatagat tggtagaaaa ataaaggagt gatattgtga gattttggtg tgagaattgt
    81961 aataaatact tcaatgcgga agaaacatta caggaatata attatttttt aaatgaagag
    82021 atacttatat gccctagttg caaaaaagat ttaataccta tagctaggga aacagaatta
    82081 tcattaggtt ttgatagtga tacaaaccaa ttaacttatg ttgaatatga ttctagtgat
    82141 tatagtttac ttagaaaggt aaatgcagat gttgaagatg tagtaaaacc tataatacat
    82201 tacataagaa atttaaataa aaatagttta gatttaaatg gaattacaga atagtgcgga
    82261 aagcccactc ctttaggtgt gggatggata gcacaaacag tgtataaaaa gtattgacac
    82321 atacacgtat aatgttaata ctttgtattc tgccctagat ataaaagaaa aatatttaat
    82381 atagaaggtt tagaagatag atttaaaaca ttagttaaaa ataagtgtaa ggaaatggat
    82441 atcgaaattg ttgcaataga atgcgataaa gaccatacac atatgttttt aaatagtctt
    82501 ccgactctaa atccttctga tataatgcaa aatataaaag gctatacaag taaaatatta
    82561 agagaagaat ttattgaatt atcaaaaatg cctagtttat ggactagaag ctattttgta
    82621 tctacagcag gaaacgtatg tagtgaaact attaaaaaat atgtgaaaaa ccagaaaaca
    82681 agatactaga aagtgaggtg aacactatgt caaattatat attaacattg caattaaaaa
    82741 cagaaaagtt tcaagaagac attatatgta atgcttttga taagtataga aaagtatata
    82801 attcatgttt atcagagcta tttaaaagat ataaccacat gatagaatct aaagaacacc
    82861 aagcaaattg taaatataag ggtaaagata gaaataaaat atttagtgaa attaataaga
    82921 agtataattt aacagagtat tcacttcata agtttgttaa atttattggt aaatattata
    82981 aactacattc tgctataact cagaagatag ctacaagatg ttttaatgct tttaataagt
    83041 atatctatca tcaagctaaa cgagttaatt atattagata taatgaatta acatcaatag
    83101 aagggaaaca aaattcaact ggtattagtt ataaagatgg tattattaaa tttaataaga
    83161 tgactattcc agtaataata aagaataatg acaattatgc tcaaagagcg atacaagata
    83221 aaataaaata ttgtagaata cttaaaaaag aaattaaggg taaaattaaa tggtatgtac
    83281 aattagtctt agaaggaatt ccacctaaaa aaacaaccaa tcaaggaaaa ataaaaggac
    83341 aaattggttt aggtaatgta ggtatagata ttggaactca aactatagct atatctagta
    83401 aatgtgatgt taagttattg gagttagccc ctaatgttaa taatatagat aggcaaataa
    83461 agttaattca acgtaaaatg gatagaagta aacgtgcaac taatcttaat aaattcaatg
    83521 ataatggtac tataaaacaa ggaaatagag ataagtggat atttagtaat cactatctta
    83581 aattaaaaag actaagaaaa gagttatata gaaaacaagc tgaaataaga aaacaagacc
    83641 actatatatt aattaacaaa ttactaaatt taggcaataa gttttatgtt gaaacgatga
    83701 gttataaaag acttcaaact agagtcaagg atactactat taatgaaaaa actggtagaa
    83761 ttaataaaaa gaaacgtttt ggaaaatcat tagcaaataa agcaccatca atgtttttga
    83821 ctatgttaga caataagtta aaatataata atgaaagatt atataagata gatactgcta
    83881 aatgtagagc cagtcaatat aatcactttt cagatgagta caataagaag gaattaaaag
    83941 atagatggaa taatggtatg gacattcaaa gagattgcta ttctgcattt ttaataatga
    84001 atgttaatga agatttaaaa tcaatcaaca gagaattgtg tgttaaaaca tatgataact
    84061 ttaaaatatt acatgataaa gagataaata gattaaaaaa attaaaatta aatggttata
    84121 aattaatatc tagtatgggg atataaaaac tatataggtt gagaaacgag cctaatatta
    84181 atgttaatgg tgtcaacaga tactttgtta ataaaagtct tagggaatat gattagtgat
    84241 aatatgttgt aggttatgat ttattataac tgagagtatt gtagaaattc atgtagtacc
    84301 taagaacccc acgcctttag gcgtaggagt ttcagaataa caatgaatgg taatatataa
    84361 gaataaaaag aaggatatta taagagatga gtcgatgtat gattatggga tgtctttgtc
    84421 atctattatg aaggttatct taaatcatca agaagatata gaaaagttaa aagaagagga
    84481 ggtagcagaa tttataaaaa ataaattaga aaatgagcat gattgtgatg aattaaaaat
    84541 aataagtgag gtatttaatg aaatatatta aacatgtaga taatggagta atgagagcaa
    84601 taggtattga taattataaa cctttattaa acatcataaa agaaaaagtg aatttagaat
    84661 ctaattaaaa cagttttaat tagcgaggtg atacatatgg gattagattt aggtttttat
    84721 tcagtaaaaa caaaagttac agatttagat atttatttaa ataaaacaag aggtgagaaa
    84781 tataaggaat tgaataatga tgaagaagag ttttataagt ttgaagaatt attagatatt
    84841 gatatatctt tagaagattg gtcagttatt cgtatgatat ataaagccaa atgggaaaaa
    84901 ggttatgaaa gttatgaaga tgaagccata cttattacaa aggaagattt ggaagattat
    84961 ataattcctt ttcttaaaca aaaagatata aattctaaga tatacaataa ggataaagag
    85021 tattattata aaaatgataa aaaagtaaca gttgatgata ggtatacaca ggagttttgg
    85081 gatttagtat taaaagaatt tcaaaaaatg ttaaacataa ttgattttga aaaacatagt
    85141 ttaattatta tgtattggta ttaagataat aattttaatt tatttggagg tttataataa
    85201 tgaaaaaaat gtacatagca attaattcag acttaaatat gagtccaggc aaagttggag
    85261 cacaagttgc acatgcagtt tatgattatt tgtataacaa aattttagac actgtttctt
    85321 gctcttatga aacagaaata atagcacatg aattaataga tttaaaatgt gatttaacgt
    85381 catttaagaa taatggggat acaatatgta tattaaaagc taaagaggca caattattaa
    85441 aatttaaaga aaaaggttat ttaactatag tggatagagg attaacagaa attcctaaaa
    85501 atagcataac atgtgttaac ttaggaatat ttaatgagga tgaaatacca tctacaatta
    85561 aaagattgag attatattaa ggagaaagac aataaatgta ttctaaaggt gatgtagtaa
    85621 aagtatggtg ttatcaatgt gaaaggatag aatttcaaga atttgtagga tattttaatt
    85681 cttattttga taattataaa ttaaagaaac gatggaaatg ctctaagtgt gatgtcatca
    85741 ataattttta agagtttaat taatggagga atgagttatg aaaataaaga aaataatttt
    85801 aggattagat aacttagaga atgtagagtt gggtattgga gatgtaggag gtttttattt
    85861 agataatata catgaaatta ttggaggatt tgctttgagt tatataggta aatgcaagaa
    85921 ggcagattat ttacatatac aaatacataa acgtgttgat gaaattcaaa gattgcatga
    85981 ttataaggat attacaaatg taaagataga atacgaagac ggaagtgtgg aggatatata
    86041 tgtaccttat aaacaagaag gggatttctt gggtttagat aatctttatc aaacttcttt
    86101 tattagtaaa caaggttatt tacatatagt tatctctaag gataaaacag tagaagaagt
    86161 cttcaatgaa gaatttgagg atgatgaagg ggtagaattt agttttaata tgtatggaat
    86221 ataaaactta aaatggaagg agatgagcaa atataattaa ctcttttaag aaattaataa
    86281 agtcaaaata tataaaaaat aacgaatcaa taaaaccatt agaaagaatt tttgaatatg
    86341 aaataagaat caaagaaaaa agaacatatt ttaatttaga attattgatt tatgaaaaaa
    86401 gaattaagat aattaaaaaa atatttttaa aagatatact atgccattat cgaagagata
    86461 tagaacaact cgtcatttat gacctggata aatatcttaa tatgacagat aaagaaatgg
    86521 aggaatatat tataaattta ataaaagata aaataaaaga agagtatggg gagaatatac
    86581 ttcctgaaat actaatggaa aaatttataa aactcgaaag aaaaggttcg gtgactgtag
    86641 acgtacggga tttaatagag ggataataaa taacaagtta aaatacaggt tttaaaagga
    86701 gaataacaaa tggaagttca agggaaatat aacaaagcta aaatatttac agagaatgta
    86761 gattcgataa caataggaca aataataaat ttatgtaatc aaaaggaatt taaagaatcc
    86821 agtataagaa ttatgcctga ttgtcatgct ggtaaaggat gtacaatagg aacaacaatg
    86881 actataagtg ataaagtagt accaaatctg gtaggggtgg atataggttg tggaatggct
    86941 actattccat tagaaaaata tattaataat ataaattata caaaattaga taagattata
    87001 agagattata ttcctcatgg atttaaaata cataataagt caagagaaaa actattaaag
    87061 aatttattta atatagacat aaataatctt agatgcgagg tgaataaaaa tagagcatat
    87121 atgtcgttag gaactttagg tggaggcaat cactttatag aagttgataa aagtgaaaaa
    87181 ggtcaaatgt atcttactgt tcatacaggt tcaagaaatt taggaaaaca aatagctgat
    87241 tattaccaag acaaagctat aaaatattgt attgaaaaat ataaaaagtc atatgatgat
    87301 gcaaaagagt ttttaatatc tactctaaaa aaagacaata aagaacattt gataaataaa
    87361 tatttgaaag accttagtga tagaaaattg aataagccac atgatgattt atgttattta
    87421 gaaaatgatt taatggaaga ttatttacat gatatgagta tagctcaaaa atacgctgct
    87481 gcaaatagaa tggttataat tgcggatata ttgtttaaat ataacagcct agaagtaaat
    87541 tatgaccatt ttataagaga tattaaatgt gatgttatag aatgtattca taactatata
    87601 gatatggata gtaaaatact tagaaaagga gcaatttctg caaataaaga tgaaacagta
    87661 ataattccag taaacatgag agatgggatt atattaggaa aaggtaaagg taattctgag
    87721 tggaattatt cagcaccaca tggagcagga agaattttat caagaggaaa agctaaagaa
    87781 aaaatatctt tagatgaatt tgaagagtct atgaaagaaa tatttacaac ttgtgtaggt
    87841 caatcaacac tagatgaagc accacaagca tacaaaccta tagaggatat cttaaacaat
    87901 ataggagata ctattgagat aatggaggtg ttaaagccaa tttataattt taaaagcaat
    87961 tagaatcttg ttatttaaat aaaatcaaaa ggagaaaaaa gggtatgata aaattaataa
    88021 cacataatga tttagatggt gtaagttgct gtattatagc aaagtatatt ttacgagaac
    88081 aaatagatgt taactattgt aattataaaa atataaatga tgtagtatta gatacattaa
    88141 aaaatcacga taaatatgaa caaatattaa taacagatat atcagtaaat gaagatgttg
    88201 ccaagaagtt agataggatt aaaaataaag ttcaattgct tgaccatcat ccaacagcac
    88261 tatttttaaa taattatgat tgggctaatg tagaaataga gtgtaataaa ggcaaaacat
    88321 gtgggactca aatgttattt aattatttac aaagatacta tgatgatact tcagaatcta
    88381 cattaaaata ttttatagaa cttgttagaa ggtatgatac atgggaatgg aaagataaat
    88441 ataatgattt agatgccaaa tatttaaatg atttaatgta tattatggga atagaggatt
    88501 ttataatgga catgttaact aaaatagata gtgatgagat gtgtctattt gataaattaa
    88561 gtttaaaatt attagaaata aaacaaaaag aaatagatag atatgtagag aaaaaagatg
    88621 aaaatataat ggttaaagaa attctaggat ataatgcagg aattgtattt gcagaaaatc
    88681 atataagcga attaggaaat aaattaagtg agttacatcc agaacttgat tttatagtta
    88741 taattaatcg agaaatcatt tcatatagaa ctataaaaga taatataaat ttaagtgaaa
    88801 tcgctaaagt atttggaggt ggaggaaatc cgaaagcatc aggaagtcag attgatgaga
    88861 gttgtataga taattacata aaagatatat ttaatataaa atcttaattt taaatggagg
    88921 tatatatgaa atttgaaaac aacatgatta aatacaattt agatttaata actatgatat
    88981 gtaagatgaa actaataatt tatgaggtgt aataatgatg atagataaat tcaattatga
    89041 attatataat gagtttccac atttatcacc aataatagaa ttgtataatt taaatataga
    89101 gtacataaga gaagttgtaa gtatacttat caatacagat atgacatttc aaaggtttaa
    89161 atattccaaa tctagagttg agtgttctat gaaagcgtat attatttcac ctaatagtaa
    89221 gctaagtgtt gtatttgcat acgattgtga attaaatgga attgacttag acaacctgaa
    89281 ggcaattata tgagtataaa caaataaaaa taatacttta caagaaaggt aaattgaata
    89341 tgtgtaagtt ttgtgaaagt ataattgata atacaaaaga tatactttta acctcaagaa
    89401 gtagattatt agaagacaat acatgtgaaa ttatagcaga agataattgt aataattgtt
    89461 atgatggatg ttatgaatat ttcaaactaa acggatataa aatcagggga aatacatatg
    89521 ttaatgtcga ctactacaaa aaaattagaa aaataataat tgctccatgt tcagaatcag
    89581 tacatataaa ctattgtcca tattgtggta aacaagtttc taaagatata aaagatttta
    89641 attgtattcc tgagcatata gtagatgtta gatataaaaa tggtgatgta tatgattatg
    89701 atatggaaaa gtctgtacaa gaagtcatgg ataatgcaaa taataattaa ataaaatgat
    89761 acttttaata ggggataaat atgaatcttt ggtctaaaat tgaagaacca gtgatgagag
    89821 cagtaagtgg aatagtattt aatatatggg gaaatgtttg tttttccata tattacatag
    89881 aagaaaataa acaaatagag ttagaaatat atgatagacc ttatagatat gaaggtaaaa
    89941 aaaatagttg ttgagaagtt atttgattac aagttaataa atgcagatta taaagaaata
    90001 catgatattg tttctatgat agaaagattt ttactagaaa attattaaaa ggagatattt
    90061 aaaatgaata aaggaattaa aaactatata acagcagtga attacttact tgataattta
    90121 gagtatatga tggaggatga cgaatttaat aatgaaaagt tacagaaaag aattgatgta
    90181 attagaaaat ttgaaagaga attataagat gggaaaaata aaaaatggag aaatagatac
    90241 tgaagattat aggcttttta aaaacattca aaaagaaaaa gatagagtat atagtaagta
    90301 cattaatgaa gaatacataa tggaagaatt cataaactac ttaagattgg aagataaaga
    90361 ttatactaca gaaatattag aaagatatgc acgatatatt gatgaaaatt tagacaatcg
    90421 ttgtccatat tgtgtatata atatttgtag tgaacatgat aattgttttg atggaatact
    90481 tgaaaattta aaagaaaaaa ttaaaataaa ataaatattt taatatgaaa ggatgttgtg
    90541 ataataagta taagagaaat tatggaaata gatatagaaa atctatcaat gaagcagcat
    90601 gaagtgatta aaatatagaa ttattaaaaa aatatgatac atatatatat aagaaggggc
    90661 attatagaga aatgataaaa gagagattta aagcaaaaga tagctttaat atggatgagt
    90721 tcactaaaat ttggcttatg gattttaaag aagattttga tttaagctta aagaaaatga
    90781 tagaaaatac gataaaaaaa tcaataaaag atacttatga agaaacagaa agggcgataa
    90841 caaatataga aaatcttata aataaactat aagtcaaaat attactaacg acacctctaa
    90901 tataagatta gttatagagg aaatctatta ttagagaagg catagattgc gtcactggaa
    90961 caattcaagt taaaaactta gataatcata tcaaataaaa tcttttacaa caaatcaaaa
    91021 tttctaaaat aaattcttat taataattca attaaaacta aaggagtgaa attatattga
    91081 aaaagttttt attaacaggg ttattaggaa caatgttaat agccatgtct ggatgtacag
    91141 gaatacaaag ttcaatgaaa gatgctgaaa gtgatgtatc agggcttaat agaactgtaa
    91201 atgtatattc agataatggc gaggtattaa aaacatatac aagtaaaagc atgagagtta
    91261 aagatggaga tggtggtact ataacactag attttgatgg taaaagggtg attatatgta
    91321 atgctcatgt agtaatagaa gaaaattcat ctaataaata aaggagagtt aaatgtttaa
    91381 aattttaaaa acaattctta taataatttg ggtagtagat attttaaact tcccacaatt
    91441 tcaattctta gatacaacat atccaatcaa taccttagca tggttgctaa tctggattct
    91501 tataccatcg agtagtatat atattgacaa aaaagaataa aagaaaggag tattaatttg
    91561 attttaaata ttgctacaat ttttaattta ggttgtatgg tggctaatta cttgcaagat
    91621 aatactaatg ggatactact taatggattt tgtcttatag cattattatt attgaataat
    91681 gacttagatt aaaagtatta ttttaaggag attaaaagat gaaaataaca atatcaattg
    91741 aagaagcaat ggatattatt aaacaacact atgagaataa aggaattgaa atcaaagact
    91801 taagttttaa tttagaaaaa actggaatgg ttagaaatat taaatttaag ggagaaataa
    91861 tacttaagta ggagatattt atatttgtta aaaaaaaggg gaagatattt ataagtagta
    91921 caaatagaag tgatgctaga agtttccata taagtgatta ttatagaact ccaattacta
    91981 aaatcaaaga gtttttaact gagttcagta gatatgaaaa tatactcaat tcaaatataa
    92041 aaatattaga cccatgtgca ggtggagata atacaaatcc aatgagctat ccaactgcaa
    92101 tccaagaatt ttcaaatcaa gaaatttcaa tagatacagt agatataaga gatgattcaa
    92161 gagcaaatat aaagcaagat tatttaaagt ttcagccaaa agaaaaatac gatgtaatta
    92221 ttactaatcc tccctttaat ttaagcttgg atataattaa taaagcttta aatgatgtaa
    92281 gagaaggtgg tttcgttatt atgctgctaa gattaaatta tcttggagga aaagtaagac
    92341 aaaggctgtg gggaaataat atgcctaaat acatatttgt tcacaataga agaatgagtt
    92401 ttacagatga tggaaagaca gatagcatag aatatgctca ttttgtatgg cagaaggggt
    92461 ataatccaaa attttctcag ttaaaagtat taataagtca atgaaataga ggtgtaaagt
    92521 ttattagaga tggaaataaa tagaagaaga tgtgagtatg ctgccagaac aggaagatat
    92581 cctaattctc ttattttgtg ggaaggttac tatagccttc ttaatatcca atatatagag
    92641 gataaaacaa gaggatatat tactaaattt aatggcatgg atgttataaa atgtaatgac
    92701 tttttaaaaa tagaattatt tgaaaagaat attaataatt aaaatttgtt ttttaagttg
    92761 aggtgatgag ttgagatata ataattatca tagtcataaa atttattcaa atataaggtc
    92821 gttagatgta attactaaac cacaacaata tatagataga gcaataaaac taggtcaaaa
    92881 tacatatttt acgacagaac atggatatca aggaaatgta tatgaggcaa aaactttatg
    92941 tgatgaatat aaattaaaaa tgattattgg agcagaattt tactatgtaa atgatataaa
    93001 tgagaaagat agaggaaatt atcatttaat agtaatcgct aaaaataatg atggttacaa
    93061 gcaaataaat aaagctttaa gtttagctaa taaaaatgga tattactata aaccaagaat
    93121 agatgaaaaa atattatttg agatatttaa accaggggat gtagttatta ctactgcatg
    93181 tgtagctgga atattaaaac ttgagaatag agaagaatta atactaaaac taaagaatta
    93241 ttttaaaaat aatttctttc ttgaagtaca atctcatcct cataaaactc aagctttgca
    93301 caataaggat gttttagaat taagcaacaa atataatata gatataatac acgccaatga
    93361 cagtcattat atttatccag aagaatcaaa atatagaact aaatttctta aagctaaagg
    93421 tataaattat cctgaagaag acggttttat tttagattat ccaaattcag aggatatatt
    93481 taaaagatat gaaaaacaaa atgtattaac aaagcaagaa gttgaaagag cattaaaaaa
    93541 tactctaata tttgatgagt gtgaagaaat caccttaata aatgatgata taaaattacc
    93601 atcaatatct aaaaatccaa ataaagaact gaaagaaata ttaaataaag aatggttaga
    93661 gaaaagaaaa aatataccta aaaatagatg gaatgaatac ttagatgcta taaggtatga
    93721 atttgatatt atagagaaaa ctcatatgga ggattacttt attatagatt ataaaatagt
    93781 tcaaagagca aaacaagaat ataatggtct tcttactaaa acagggagag gttcagcacc
    93841 ttcatttata ataactaatc ttttagggct tactgaaata gatagattaa atgctccagt
    93901 accattattc cctactagat ttatgagtgt tgaaagaata ctatcagcaa aatctttgcc
    93961 agatatagat ttaaatgctg aagatgctga accatttata caatcaacta gagatttatt
    94021 aggagaagaa aattgtgcat ggatgataag ttataagcca ttacaagatg cttcagcttt
    94081 taggctatgg tgtaaagcaa atgatatgaa actatctgag tatgatgaag tagctaaaaa
    94141 cttagataaa tatacaaatg atgttttttg gaaagatgta ataaaggaat caaaggtatt
    94201 tgtaggagta gtagaaagtg tttctttctc accatgctct atgcttatat atgatagacc
    94261 aattgatgaa gaagtaggat tattaaaaac taaggatggg gtatgttgta atatagatgg
    94321 atattattgt gataagtata aatatttaaa aaatgattat ttaacagtga aagtatggtc
    94381 attaataaga aaaatttgtg aattagcaaa tattagtatt cctacaattg aagaactaaa
    94441 catcttatta gatgctaaaa cctatgacat atataaggac aaattaactt gcactataaa
    94501 tcaggtagat tcagactatg ctacaaacct ggcatctaaa tacaaaatat ctagtgtggc
    94561 ggaaacaagt gcatttgtag ctagtatcag accaggattt gcatctttat tagacaattt
    94621 tattaacaga gagtcatata caactaacgt taaagaacta gatatattat tagaagatag
    94681 ttatcattat cttatgtatc aagaatcaat aatgaaatat ttaatatggt taggaataga
    94741 ggaatctgag agttatgata ttataaataa gattaaaaaa aagagattta aggaaaaaga
    94801 gttgaaagaa ttgcatatca aactaaaaaa taactggatt aaaaaagtcg gaactgaaaa
    94861 taattttgaa gatacatggc aagttgtaaa cgatgctgcc tcatattcct ttaatgcttc
    94921 acatagtctt tcatatgcat atgacagctt atactgtgca taccttaagt cacattatcc
    94981 attggaatat tatactgtag cattcaattt atataatgaa gatacagaga gaacaagaaa
    95041 attaacagat gaaatagagt attttagtat taaattagaa aatcctaaat ttagattttc
    95101 aaaatctgaa tacttcttta atagagacac aaatagtatt tacaagggca ttgaatctat
    95161 taaattttta aattctgata taggagagta tctatattct cttaaagata ataaatatga
    95221 ttcattttta gagctattaa tagatttgca ggggcatata aactctaaac aattatctat
    95281 actgattaag ctagatttct ttgaggaatt tggaaagtcc aacaaacttt tagagactta
    95341 tgatatctat aactcaatct atagtaaaaa acaatttaaa agagataatt taccatgcga
    95401 tataaatacc atgagaaaat actctaataa agaaacagaa aaaatattta aagaagttga
    95461 tacaaaagaa ttatgttctt atcttgaaag tcaaattgaa aatacagata taccaaccaa
    95521 tgaaaaaata caagctcatt ttgagtttgt gggttcatgt aacataaaag atagcaattc
    95581 aaatccaagg tattgtttag taatagatat agatactaaa tattctccta aagtcacatt
    95641 gtataatata agttcaggaa atataaagat tttcaaagta agtaaaccta tttttgagga
    95701 taataaaatt gatgtttatg atttagttta tttaaaatca attaaagaaa aagctaaaag
    95761 taaaaaagta gatggaaaat ggattaggag caatacagaa acagaatggt ggatacaaga
    95821 atattggaaa atagaaaatt aagaatatga cctcgtatat gcgtttctaa gagtttacat
    95881 aagagtctgc ttgtatttat acattgattt cttaaaatgg catatacgaa gttggtgaaa
    95941 ggtagtgatt tcaatactta tagatgaaca aagatattat agcttattgg aagaaaaaaa
    96001 agacctaagt atgagaatcg atgaattaaa agaggaaaat gcttttttaa aacaagaaaa
    96061 tgaagagtat gaagaaattc ttaatgagtg ggaatgtgta agaaaagaaa ttgtagcaac
    96121 ttgtaaaaaa aaagaaatat taggtgaaaa accaagtgaa ttagaaatgg ttcttcaaaa
    96181 aatagaggat atataatgag tgtaaataaa atttatgaga aaaaagaata cattattatc
    96241 aaatcaaaat ctggatatat agtttataat acaaataaag agtttaaaga aggccacact
    96301 cacttaaaaa gttataatgc tgctaaaaca gctatagatt tagttattaa gagaaggctt
    96361 cctaaaagtt attctattta ttttcttaca agtttgatga gaatatctaa tgatgtacaa
    96421 tatataaaac atataagcga tattgtatat ataagaaata ataaaggact aaaagaaaaa
    96481 tatataaatt ctaaaaatat taaaaaaaac tattgagaac atagaaggta tatgatatac
    96541 tatgaatata acaagaacat agaaggtaaa aaggaggtag tgattaatat ttgaaatctt
    96601 ataaagaaaa acagttttta gtatttgaat ttgaagatgg gaagaatgta aagtataatt
    96661 tggcaacagg agaatacgtt ggtaaatctg gaaggattgt aaaagatgtg tgtactcaac
    96721 ttagaggata taatttacat gaggtaataa attcttttga agatgaaaat tataagtgtt
    96781 ttttaaattt tgtagatgga agagtaaata gaagtaagag tagcagagct tggggaagga
    96841 gggttgaaag aataagaaat ataggaagtt ttttaaatga aataaataat tattctgagt
    96901 atgaacaatt attctcagca ggattaaaaa aaataattta tccgatacat tgtgatatta
    96961 agagtatacc taaaggtctt atttctatct gtaggaatta tagtattact ttgagtgacc
    97021 gattgataca agggtatagt aaaaacccaa atttgtataa taatttatta agtttagaat
    97081 tgaattcaat aagaaaaact aatttaattt caattcttga ttctggttat gatagaatga
    97141 tatttgatag gttaatagac atatataaat ataaacctca atcacttata aattatatag
    97201 ataatctgat gacatatgaa gcattaaatg ggcttgaaag cactctaaac gaattatatg
    97261 actatgttgt tatgatgagt gtaataagtg ataaatatga aaaatatcca agacatttct
    97321 taacaactca taaaatagca tctcgaaatt ataatcgtct taaaactcaa tttgaagaag
    97381 aagtatttaa taaaagaata gacaagtcac tagaatattc atatcaagat tataaattta
    97441 tataccctaa aaatacaaat gaaattaaag atgaagcagt acaacaaaat aattgtgtaa
    97501 gtagttatat tcaaaatgtt atagatggaa aatgtcatat attatttatg agaaaaaagg
    97561 ataatccaag taaaagttta gtaacaattg aagttagaaa tggcaaaata gtccaagcta
    97621 aaggtaaata taatagagat gtcaatgaaa gagagcaaga agcaattgat aaatataata
    97681 atagaataga aaggatgaag aaaatatgtt aatagaagga actaaaataa gattaatgaa
    97741 agaaatacaa ggttttgaga tgttaaaaat aggagatata tttgttatta catctgtggg
    97801 aaacaatgga gctatacatt tcaaaacaaa ttatgggatg ggattcatga attatagtga
    97861 attaaaaaaa tattttgaag ttgcaataga gactaagaaa aaagaacata tttggagcga
    97921 atggagaact gcgactgtat ttggaacaaa taaaacttgc acatatagaa caaatggaaa
    97981 gaaagtagaa gttagaatgg gagattttaa agcatcagct acttgccatg attcagatga
    98041 gtttgaatta aataaaggtg ttgagttatg tctggcaaga attgaagtta aaaaagcaaa
    98101 taaacaggtg aatttagtat tagataaaat aaataataaa gaaatttagg aggttagaat
    98161 gagttgtaaa tttaaagaaa gatgtgagaa taaaaataga gattgcgatt attgttgtta
    98221 taatccaaat gcatatttag aagatgcttt tgaatggaat ggagaaggga gaaagccaac
    98281 agacgaagag ttagatgatg ctcttgaaga ataaataagt atttaaaatt atcattttaa
    98341 tatgattgga ggtctttatg aggtggagaa atatattagt aacaagtgtt ttagtaatgg
    98401 gtagtagcat acatatattt gctaatacta aagaaatgag tactagcagg atggataaaa
    98461 atattgtagc agatacagct aaaaatatta acttaatgta taaatatgag tgggagcaac
    98521 acaaaaatag ttctgaatat aaattagaaa tcaagagaat taatttagaa aaggacttag
    98581 gaataaaaat agaaaaattg attcctgtaa catgggaagt aagttactat acttctttaa
    98641 actgtgaaaa tagtaaatat ggagctatta cagctacagg agaaaaatta caatatgggt
    98701 ttgtggccaa taattatctt aaattcggaa ctaaaatatt agtcgatgga aatttaaagg
    98761 tggttaaaga tagaggttct aataaatatt ttggtaattc taatgctata gatgtatttg
    98821 taccaaaatt agagggggag agtgattata aatattataa gagagtaaac aacatgggaa
    98881 gacattataa agaaggatat attatagttg aaggttagga gttgataaat tgaatgtaaa
    98941 tggaataaaa ataccaggaa ttattgatgt gtcaaatgtg tatgtttatg gaattaaaga
    99001 aagcataatt gcaagtggtt atccgatgta tgaacaacag gtaatagata tgaaaaaaat
    99061 aaacatttca gaaaaagatt taaaacgagc aaatcaatta ggcaaatcaa aacaaggttc
    99121 aggtcatgat tgcttttcta aaggaattat cattcaaatg gatttacaat ggtcagaata
    99181 tatgtggaga caatgggaca gatatcatca taatgattat gtgtctagtc aaagtaaaat
    99241 gcatagatta tctaaattta atcttgatga tatgtgtaat aaatatgttg ataaaaatac
    99301 aataaatttt ctaaatgaat taattcatga gtacaataac tttgaagaat tgaaattgaa
    99361 taattctaat aatattattc ttagaaatgg agaaaaagtt gaagtcacaa aagaaactct
    99421 ttggaaaatc ataatatcaa attgcccatc aggattgatg ctaactgcaa gagttacatt
    99481 aaattatcta caaataaaat ctatgataaa tcaaagagaa aatcataaaa tggaggaatg
    99541 gagaattctg tgtgactttt ttaaaacact accaaaatat aaagaattaa tgatgtaata
    99601 gttttgttag caattaaatt ttaaatataa aggagaagtg ggtatggcag aaaaaagaat
    99661 aaattataat caagtatttg tatcaggaga agttttagac attttaaata tagctagaat
    99721 gaatcaagga actggccaag aagctataag atttacctta aaggttgaaa cagcaccaaa
    99781 cgaatcagta aatgtagatt actttacatc aatgtttaag tcagatggtt caccaaatca
    99841 aatgttttta ggaatagaga caatagctaa tgaaataaaa actagagcag aagatggaaa
    99901 aggtgatatt gttagatgtg tttgttcatt agacaataac ttatattata aagatggtga
    99961 aaagaaagag cgattccaaa tatcaggaac tttttgcaat agagaaaaat atgatgacaa
   100021 aggtaatcct attgatgaca aaggcgaagt agtaaagtca ataaaagcga gtcaggtctg
   100081 gagagtatat acattaattg aaaatataga agaaaaagaa gatgatttag gtaagtattt
   100141 agagattaag ggtttaatta ataaatatgg taagcaaggt tgtaatatga catttagaat
   100201 acataatgaa gatatgatag aaggatttaa aagtttatat aaaataggag atgttggcct
   100261 tcttgaaggt acagtaaaat ctatggttgt tagtaaatca gcaggatttg gttcaagaat
   100321 taaaaaaagt gcttttacat ttttagaaat agaaggtggc gatgaacctt tgaaagatgg
   100381 agacaaaata tttagtgata aaaattatcc tttcacagat aagaatatag aggcaatgag
   100441 agaaaaaata aaagaaaaag ataaaaaaga aaaacaaaga gatattgata aaaatggtaa
   100501 gacaattgag ataagagatg atgatgtacc attttaaatt ataaattaaa taatctatta
   100561 aaaaggagag atgtgaatgg aagttaaaga aaaaacaatg aatgatggtt tcaagaaagt
   100621 ggtaaatgag gaaataaaga cgtatatatc aaatttatat tcagatagaa tagacggatt
   100681 aaaagagagt gatagtcaaa ttgagataac aaaagcatta atagcctttc atttagatat
   100741 agaatcaatt ccaaaggata atacaaaccc atttttcagg tctaaatatg caaaccttga
   100801 tacaattctt aaagcagtaa atccattatt agcaaaacat gatttaatac taatgcaatc
   100861 agcagaagat gcaggtaatg ataaagtata tataaaaact aaactaaagc attcatcagg
   100921 cgaatacata gagtcaaatt ctgcaccatt taaaccagct aaaactaatg atatacaagc
   100981 tagaggagca ttggaaactt atttgagaag atatgcagtt caatcagtat tagcattatc
   101041 attcgaggaa gacgatgatg gaaattcttt aacaaacaaa tcaaagggta aatcagaaga
   101101 agaaaattca actattaaga aaagtagagt gtaaataaaa ttgctacctc ttaaatgagg
   101161 tagcaataaa tacataacat aaggagggga aatcttgaat tgtaagtgta ggttttgtaa
   101221 aaagaaatta aatacaaatg atgcttataa agtagagcat attacatctg ggggaaataa
   101281 gcagaataga tattattgca atgaacagga atatagaaaa gagcaacagg atatatattt
   101341 ctggaaacaa tgtcaacttg gaatagatta tattatggga tatacagtta taaataatca
   101401 aaaaaataag atgttacaag agataataaa aggtggttat acgagagaag aattatatga
   101461 ttgcatcatt gagaagaaag atgaagttat agaattactt aactatagaa aagatataga
   101521 agaagaatat cctaaattat gttatgtgtt tacaatattg aaaggaagta ttagagatat
   101581 aacaattaaa aacaagcaaa tgaaatatga aaaagaaaat gaaaggatat acaaagaagt
   101641 agagaaaagt gaagaatatt atgaaataat tgctcctaaa aaagtaattt ataataaaag
   101701 acaaagttta tttgacaaag tgaaaggagt gtatcaagac gagtagttat gaaaaaataa
   101761 tagaggatag gggtattata gaaacattgg ttttaggcac aatgttaaaa tcattaactc
   101821 tttttagtga atataaaatt agcgaaagtg actttataat agataaggta aaattctttt
   101881 tctcgttagg aagaataatg tctaaaacac ataacgagct tgatgaaata agtgtagcta
   101941 aatttgtatc ttcaaataaa ctcaaatctg aatatgaaag atatggtggt tggaatagtt
   102001 tatcatctgc tatggagtat ggaaaagaaa ctaatatagc agcatatatt gatgatttag
   102061 ctaagaataa tttactaatt gctttagata aaagaggttt taatgtaata aaagaaatgg
   102121 agcataatgg gttaaaattt attccatttg aaatatttca atctatgaaa tgtagagaag
   102181 tggaagaatt ttatgagggg ttaatatctt catgtagtgt aaattctata aaaaataata
   102241 tgaaagttga aaatctttta cttactaaag atgatagaga aaaattaaag caaaaaactg
   102301 aagcaggaac accttataat attatatttg aatacactga aaaagaaata ggattgagta
   102361 ataatgaaga atctaagtat atatatagct taccaatatt atcaaatagg actaatggga
   102421 ttggtaaggg tggtggtata aatataatag cagggttttc aggcatagga aaaactactt
   102481 tactattttt taattacata ttagctatga tatatagagg agagaagata gtcatatttg
   102541 cgaatgaaca aaaatctcaa tattttaaga gtatgttagt atcgtttata gcatataaca
   102601 tatttaatta tcatgattta gatagaaata aaatagataa tggagatttt acagactttg
   102661 aagaagactt aatggaaaaa atagaaaaat ttttaagaga tagatgtttt gcagagaatc
   102721 taaaatttat ctatatggaa gaatttgaaa tatctgaaat actaaggaaa agtaaagaac
   102781 ttgttacaca tcagggattt actggaatag cagtggatac atttaaatca gaagattcgt
   102841 cagatgcaca ttatacagga aaattaattg aaaattctaa attactcgat agttttggga
   102901 acaaatataa tgtaataact atgttatcta tgcaattact tacagctcaa gaaaataaaa
   102961 gctcatatct ttcggcagga gatttgtcag aagcgaaagc tgtcaaaaat gtatgtggtt
   103021 tattaaaact tatgagaaaa gtagtaaatg agttagaatt agattctact aacaagaaat
   103081 tctttttaaa accatataaa ttaaaatata ataaattaaa aaagactact gaaagagaat
   103141 atatccagtt tgatgcaaaa gatttgcaaa aagaatatag attattattt ttaaataagt
   103201 caagaagagg tcaagatgga gatgtaattt tactaagatt ttatggtaag actggtagat
   103261 tagaagagat aggaaggtgt gagaaggtct acagaggaca attgtcatac taggtgatga
   103321 ctatggaaat aaaggatttg actaatgaac aggtgataaa ttttatggaa tatttaggtt
   103381 cggacttatc tcctaaaagc aatgatagac agttaatatt caatacttgt ttatgtcata
   103441 atggtgattc atacaaatta ttttattata cagaaagcaa aacgtttcat tgctatagtt
   103501 cttgtggcca tataggtagc ttaatagatt tattaataca cataaataaa tatgagttta
   103561 aagatgctat taatgaaata aaagatttct ttggtatttc aaatcaacca atgctaagaa
   103621 aaggatttag aataaagaaa aaagtagaac agatacatga tattaaagat atacaaatag
   103681 aattacttcc tacccctaaa aagccatatg tatataaaac ttttcagcaa gtcccaattg
   103741 aagagtggga aaatgaaaac atatcttttg aagtccttaa aacatatcaa gtatattata
   103801 atctatatga aaatcaaata gtaattccac atttctgttg gcatgataga gctaggcctg
   103861 ttggtattag agttagaaac ttagatgaag ataaggctaa aagttttggc aaatatattc
   103921 cattatggta cgacaatagg tgctataatc atagattaag tcttaatcta tatggattaa
   103981 atgtaaataa aaaatctatc aaaaattcca aaaaagttat tgtatttgaa ggtgaaaaat
   104041 cggtgttaca aatggctact atgtataaaa ataatccctc tgtagctata tgtgggagta
   104101 attttagtag agagcaaaaa aagatattaa tagatttagg tattgaagaa ttaatcatag
   104161 cttttgatag acaatttaaa gttaaagatg atgaagaata tgttatatgg agagataaga
   104221 tatataaatt agttcaagat attaaagatg tagtgaatat aagtgtaata tgggataaat
   104281 ataatttatt gggctacaaa gatagtccta gcgataaagg aaagacaata tttgaaaaac
   104341 tattaaaaaa cagaattaat atagagaact ttgttaaaca ataaaaaagt aaagaaatta
   104401 attttatgag aaaattttta ttgtttaggg atttattagc agatgctaaa taaaatatgt
   104461 gttttaatat gaattggagg tagattacat gataaaattt ttaataaaaa acgtacacat
   104521 gggagataaa ttatattgta taaataacat aggaaattat aaaaaaggtg aaatttatac
   104581 agtgagtgat actagttttt ttggactctt tcataaaact aaaggagaaa attgcttacc
   104641 aattgaatgt tattttatac acttaccaaa aaaagaagag ttagaaagta atcctaaatc
   104701 aatgactaaa gaagaaattg aagatgaact aggatataaa attaaaatag tttctaaata
   104761 ataaagtgag gtgatattat attgagtatc ttcaataaaa aatatggtag aaatgtaaaa
   104821 ataacagaag aagaacctcc ttctcctcct cccaaacaac caatatataa tagagataat
   104881 atacaatatg ataaagaaat ggaaagaaaa ctattagata ttgataggtt aaaaatagaa
   104941 ttggaaaaag aaaaaaagat agaagaagaa agaaaaaaag tagaaagagt aaaggaaaat
   105001 gagggaaagg aatgggcttg ggttgaagga tataaatgta cagataaaaa tatgaaatgt
   105061 aaggaatttc aatatgaatt aaacaaagcc tatagtatgg atggtgatga tattaatata
   105121 tggaattggg gattccattt atgtttagac ttgggtgacg tatttcaata ttatgctcca
   105181 aatttaagca atagattttt taaagtaaaa gctctagtga aaaaagccga taaatataaa
   105241 tatggtaaag aatatggtga agtgactctc ctttcaggta gtgttttacg tggtcataaa
   105301 ataaataaaa tagctgctaa agagataata tttttagaag aattaacttt tgataaatta
   105361 aaactacata tacaaaatag ttttcctgaa gtaaacacta agtctaaatg gtatgccata
   105421 tgtaaattag gagaacaaga atattatcat agaatgttta tgaatcaaat gaaaaagatt
   105481 agattttcag atacattttc tcaattatta tttgatgaat gtcgtaatta ttgtgaaata
   105541 ttagaaatct taaataaagc aatagcatat tcaaaagaaa atttatctaa agatatgata
   105601 atatatttgc ttatgaaaga tataagaaag taacatataa aaagaaggga gataatgtgg
   105661 acaaaataaa taagatagat ttgattaagt taaaagaaat gaatatatat gataattatc
   105721 aattaaaaaa aatagcaagt aaaatgatat tacatctaag atatgaaata atggactttt
   105781 gtggttgtgg ttctcctgaa gatatatctt ttatgataaa aggagtattg actgccatac
   105841 aaaataaaga aaaaaattgt aatttagaat acacagaaag atgttacata tttgaaatag
   105901 aatttaataa tgtttgtgga ataattggta gtaaaaatga tttgatacaa gaatttatat
   105961 taaatgcttt aaattcatat gggttattag aacatggtag tagcatttgg gggtcatggc
   106021 tatctgatta tggtaaacaa atattatgtg cttttgaaat agttggtgac tgtattttag
   106081 atgttagcta tttagattaa aaagttagtt ttaataggag gtggtaatta ttaaatatgt
   106141 aatgagtgac ttacatggta tgtatgataa atttatttca atgttagagc aaatcaattt
   106201 caattcaaat gaccatttgt atattttagg agatgtatta gatagagggg ataagtcttt
   106261 agagattata gattatataa gaaagtataa gaatataact cttctaaaag gtaatcatga
   106321 gttaatgttt caagaaagtt atacagatgt aaatagtagt tttctttggt tttataatgg
   106381 aggtaaagat acattttatg atttaggaat gaagtcttat gaatataaag aaaattttta
   106441 taaatatatt aagaaccttc catatttaga gataatagat aattttatac tagtccatgc
   106501 tggactatac cttccaaata actatgagaa tctcagtata gagcaaataa ttgagttaca
   106561 agaagaagat atttgtgtgt gggatagaac aatattaaat actaataaac atataaaagg
   106621 atacacaatt attattggtc atacaccaac tcaaaacatt aaagattatg aaaaggctga
   106681 aatatataaa caggaaggaa ttataaatat agattgtgga gcatgttttg ataatggtaa
   106741 attggcttgt ttaagattgg atgatatgaa agaattttat atttaagagg tgttatatgg
   106801 acatattaaa aatcagttac aacaatctaa atgctatatc aaaaatatca gaagtattaa
   106861 gggatattgt aaatgaggac actttaatag tatgtatagg tagtgataga gttttagctg
   106921 actcattagc ccctatgatt ggcagtatac tagaaaaatc tactattaga aataaaatat
   106981 ttggagtatt aggagattca atacatgctt taaatttaga acaaaaaata caagcaataa
   107041 aaaataatta tccaaattca aatataatag ccattgatgc ttgtataagt aaaatttcag
   107101 ataaaggaac aattataatt agcaataaac ctgttaaacc aggtttgggg gtagataaga
   107161 agttactaga agttggtgat tattcaatag ttgggattat aggtagaaat aaatatgata
   107221 tatatgatac ttcagaccct gaacttatac ttgatttagc tgatgttata tcgaaatctt
   107281 taatatctat attattagaa aaagaagaaa ggatgattgt atgatacagg aagtcagaca
   107341 agaaatacta ttaaaaaatg gtttgatttt atatacaggt gatttggtag aaataaaata
   107401 caaatcagat gaagatgtaa tagaacatac ttgtaaaggt aaaattaaag aagcaaaaga
   107461 attatttatt aaattagata caagtaagaa atataaaact tctgaaagaa tgatttattc
   107521 ttgggaatta aaagaaataa aaaaggtgga tgatgaagat gaaaaagata attaatagta
   107581 aatatagagc attatattta tttatatttt ttctaatttt caatttaata tatagtttat
   107641 attttggaag gaatacggag attggatttt attctagtac atcttgcata gaagaatata
   107701 tactggatat tataacctct ataggactat ttttctcaat gatgttagct gggtttgatg
   107761 ttacagaaaa ctttataaac agtttaaggg attattgtga agaagatgca aagtgaggtg
   107821 aataaattgg aattaaagga ttatataagt aaagtagaat taaatgacat gccattgacc
   107881 ttagacaata aatacatgtt aaatgaaata agcatatctg aagatagcaa aatctttgct
   107941 gtaaaagaca catcaggaat aacatcttat tatactgaaa tagaatttaa taaactattt
   108001 agaggaatta atagcatcct agagaatatt atctaatata tatttttaag gaactgaggg
   108061 ttgagagcta ttaattaata gctctctatt tttttgaaag gggagaatta atggaaaagg
   108121 aactatacag ctttagtaaa ttaaatgcat ttaacacatg tccatttcag tactatctta
   108181 catatgtaaa aaatttaaat cgagaacaaa gttgttacgg ttattatggc aatgaattac
   108241 atagtttact agaacaatta caacaaagaa aaataacaaa tcaagaagct attcaaaaat
   108301 acaatgaagt tatagaatat gcaaatctaa tggattacaa ttttcctaca ccaaattcaa
   108361 gaattaatta tttagaatgt atattacatt atatagaaaa ttttgtacca attgaatgtg
   108421 atagatacta tatagaagaa tattttgagt ttgatattaa tggaattaca atgaggggat
   108481 atatagactt atattatata ataggtaata aaatttatgt tatagattat aaatcgagta
   108541 gtaaattttc taaaaaagac ctacctaaaa aatcaatgca gcttatctta tatgcaatgt
   108601 atcttaaaga aaaatatcca gataaaataa ttgaatatgt agcttttgat atgtgtaaat
   108661 atatgaataa tgaaaaagga gtattaattg aaagaaataa gattgatagt ataaatgatt
   108721 atgaaagagc tatagtaaaa attaaatata ctaaagaact agagaaacaa ttaattgatt
   108781 ttgttgttga tacagttact caaataaaac agctagattc caacgatgag agcgtttggt
   108841 gcaaaagtga tgacaaaagt aatcagtttt tttgtaaaac tttatgctct cattataaca
   108901 agggttgcaa acataaggaa aacaagagtt ttagaaaatc aaaatcataa taaaatgtag
   108961 attttaataa gattggagag tgtttaaatg aatataccaa agcaagtaaa agtaggaggt
   109021 ttattctata aaatagaaga gacagaagaa cctattttag taaataatca attatgttat
   109081 ggattagctg attatggaac tgaaacaata aaattatcta ataatctaca atcagaaaga
   109141 gtaaaagaag ctactttctt acatgagtta ttccattgta tatttagaga tagatgtata
   109201 gagagtgaag atgaggaata tttagtagat gtattaagca agggacttca tcaagtgatt
   109261 attgataatc ctaatatatt tgaacttaag gtgagtatag atggataaat taaaaaatat
   109321 atttgtattt ttaggttatt ctggctcagg taaagatagt atagtaacag aaatttcaaa
   109381 gcaattcaat attccaattc taatatcaca tacaacaaga cctccccgta attacttaga
   109441 aatcattaat aaaacctatt attttgtaga taataaattc tttaagaaag aaaaaaataa
   109501 ctttatagaa atgagaaagt atatagttca tgatggcagc acttggctat atggtataca
   109561 taaatctgaa ttagagaata aaaaatatgc tttgactata gtagatgcaa gtggatataa
   109621 agcactagaa aaatatttta caggaacaaa aactaagtta gtaccatttt ttataaacgc
   109681 agatgaagat actataagaa aaagattaat agatagaggt gataatctta aagaaatcga
   109741 aagaagatta gttgatgata agattaaatt taaagagttt ttaaatagtg aaaactacat
   109801 tgtaatagca aataacttaa gtataactaa tgcagtagag caagttaaaa tacatatgaa
   109861 agaggggatt caatggtctt agccttagat gtatccatgt cctcaactgg atgggcagtt
   109921 ataaatagaa ataagaatat attaaaatat ggaaaaatag taactaaaaa agataaattc
   109981 aagtctgaag atgagcgaat gtgttatatc tgtaatgcaa ttcaagagtt aattataact
   110041 cataatattc aaatagtctt aattgaagac caattcactt caagaaactc taaaacaatt
   110101 cttagtttaa gaaaactctt aggagcaata atgaggactg taaaattaaa taatatagag
   110161 attgaatata tgtatccagt ttctattaga aaatatctcc tgaataatgg taaagctaaa
   110221 aaggaagaag tagctgcata tgtaagagaa aacattattg atattggaga atatattgat
   110281 aggacttgta aggctaaaac aagtgatata tatgatgcta tagcacttgg aatagcttat
   110341 ttaaacaaac ttgatactaa tagataattt taaaaaggga gattaaatta atgagtataa
   110401 atgttaaaat aaagaaatta aatcaaaatg caatagtgcc atcttatgca aaagaaggtg
   110461 atagtggaat agatttatat acactaaaag atacggtgat tccagctaaa tcagcagtag
   110521 caatatctac aggtatatca ttagaaattc catatggata tgaaatgcaa gtcagaccta
   110581 gaagtggtat aagcttaaaa ggaatgtttt ttacaagata tgtgactata tatgataaag
   110641 tgcaacctaa aaaacaaatt agaaaaattt ttgtagcaca tccaactgta agattaggta
   110701 cagtggattc aggatataga ggtgaaatta aaataattac atataatgaa gaggattttg
   110761 atattttaat tcctaaacat actaaacttg cacaaggagt atttcaacaa gttccaagag
   110821 ctatattaaa agtggtagat gaattgacta gctctgaaag aggaagtaat ggatttggaa
   110881 gtacaggtat atagatgaat aagtatataa tcctagaaaa atataattat tactatgatg
   110941 acaaatgtat gacattatat catttagaaa atatctatac aggattaaga ttttctattg
   111001 aaaaaattga attagagtac tataatatat cctagactaa ctttgggagg agtattaaat
   111061 gtacagatat gatgattatg taaatttaat aaatgactta gaattaccaa aagaaattag
   111121 gaataaatgt gttagagtta taatgacact agagaacatg caggatatta tggtagtaca
   111181 taagtttaag acttatgttg ttaatgaaaa atatattgaa aaagtagcat aaaataatta
   111241 agtcatctat taatttaggt gacttttttg catttatgtc gaatatttca tctacttcat
   111301 attgtattag atgctataat taaaatatat gtcgaaaggt agatggtgag gatgtttaag
   111361 gagatatttg aaaaggtatt tatgtcagaa gaagttagta aaactgtaaa agaagttaat
   111421 aatattatta aggatactaa gatacaagta aaaggtggat ttggagaact tagattagat
   111481 tttatactta gcagattagg caaagattac ataataatta aagacattat tgtaccaggc
   111541 tcaaataaga ctacacaaat tgatagtatt gtgatttcta tgtatggaat atttgttata
   111601 gaatgtaaaa attttagtgg atatatttac ggaaatgata aagataaagt atggactcaa
   111661 attgtaggaa aaactaaaaa tacattctat aatccggtaa gacaaaatta tgctcatatt
   111721 aaagcaatag aaaatataat aggaaataaa tataatatat attcaataat tgttttttca
   111781 gacaaagcta cactaaaaaa tgtaatgata agtggaaata atattatagt tataaatgaa
   111841 agtgagatat taagtacaat aagtaaatgt aaagatgtga ctattgataa agaagaaata
   111901 aaaattatca gagataaaat ttttgattgt atgaaaacca ctaatcaaaa tactagagag
   111961 catgttagaa atttaaaaaa tattactgaa gaggataagt gccctagatg tcaaattgga
   112021 catttgatta aaagaaaagg tcagtatggt gaattttggg gatgtagtca gtatccaaaa
   112081 tgtaaataca ctagaaaata aaaaacaacc tcttaaattc gattttaagg ctttgtaaaa
   112141 attaatgctt ataattaatc ctgttcggaa aatcgattgc ttaaaatgga aaatatataa
   112201 aaataatagc gtgatagtgt aaggcataga attgtgtatt ctatgtctta tttctttaca
   112261 actaagtaat ttcaataatt atataaataa aagaattaaa aaagtcagtt gaaagtcagc
   112321 taaaatgact attttttgcc tcctctgtat ggtataataa gtatatgaat tttgatagga
   112381 gggatagtca tgaacgcttt tataagaaaa agaaataaaa attatgtagt gtacttagaa
   112441 tttagagatg atgaatcagg aaaaagaaaa caaaaaaata tgggagcatt tgataaaaag
   112501 agagatgcta ataaaagatt ggctgaagtt aaggacagta tatataaaga tagttttctt
   112561 gttccaaatg aaatcactct agctggattt atattagact ttcttgaaaa atataaagat
   112621 aatatttcgg catctacata taaaagctat attgctattt gtaaaaatca tattaatcct
   112681 tctattggaa aatatcgtct tcaagagttg agaaatattc atatacagaa ttatatagat
   112741 gatttagctg gtaacttaaa tcctcaaact attaaagtac atataaatgt attgagactt
   112801 gcaataaaga gagcgtatag aattaagtta ataaaagaaa atattataga tggtatagaa
   112861 agtccaagaa ttaaaaaatt taaaaatgaa atttatgata aagagcagat gctaaaatta
   112921 ttagagttag ctaaaggaac taatatggag cttcctatta gtttagctat aggtctagga
   112981 ttaagacttt cagaagtttt gggattaact tgggataata ttgattttga tgaaaataca
   113041 ataacagtaa ataagataac tagtagatta gatggttctg ttatacttaa agagccaaag
   113101 acagaaagct ctgttagaaa aatatttgca ccaatagagc ttatgaattt actaaaaaat
   113161 tataggctgg agcagaataa gaaattattg agaagtattg ttagaaatga atataactta
   113221 ctattttttg atagaaaagg gaatccaatt gctgaagatg taatgagtaa gaaatttaga
   113281 aaattcttag agaacaatga tttacctcat attagatttc atgacttaag acattcacat
   113341 gttactttac ttataaattc caaagtacct ataaaggtta tatctgaaag ggtgggacat
   113401 tcaaatatta atactactct taatgtatat tctcatgtac ttaaagaaat ggacaaagaa
   113461 gcttctgata gaatatctga aaacttattt aaggctaatt aataattatt atcaataaat
   113521 acaaaattaa taataaaagc aaaggtataa acataaaggg gaatttttac aaagccttag
   113581 aatggcatat agagcgtcag attttagaag atattaaaat tttatatttc caatatttat
   113641 gatataataa acatagcaag gaataaatat ttgaaaaagc tgtgagtggt gtttccatag
   113701 aaatttcctc actttcttat gaaaggaggt gaaaaactat ggaaaatcta atgatgagta
   113761 ttatagctgg tgttatagct agttatatct acgataaatt aaaaagccac tcaaacgcga
   113821 ctaagagtgg ctgggaactt aatattaagt tccaaagaaa atccaaataa actaatttag
   113881 tttggaaaca tcactcaaag ttaagtaaaa tatgtttcct tgcttttatt ataccacaaa
   113941 ttaagaatat taaaacaaaa tttcatgtaa ataacattta atctgtaatt tttctatttt
   114001 caaaaaaaac taactaaaag ttgaaatata acaaatccaa ctgttgaacc taaagcattt
   114061 ataggtactg atgttatatt agtaaagtta tttaaagctg atatccctag cattactata
   114121 gaaactatag ccaaataaga gataactaaa aaaattggta ttgatatact tatattaatc
   114181 tctaattcag gcaatttcat aatatcattc ctttcaacat tcttatattt aaccttatta
   114241 taccattaaa tataagaaat cataatgtac aaaataactt atttttaatt acatactatg
   114301 aaagagagtg cttttaatgg ggtctatgtt ttatatatac tattccttaa aaatctattt
   114361 aaagaatact atcatagaaa catatcttgt tagattttga gttaaaagct acattatttg
   114421 attcatttcc tatctgaaat tatattgtat gtaaatcccc tgaaaattta tggttaaata
   114481 catattcttg attatcaata gtaactattt ctgtaaaatc ttttttagaa gcattataag
   114541 tattgttttc agttagataa gtattgtttg tctttgtaaa attaacactc ctatctatga
   114601 atgataattc aacctaactt aatcatattg ttgtcacaca acctaatgca attaatctac
   114661 tctgtatttt tttatcttgt tataccctct acttcaattc tattttaata gaattttatc
   114721 atgtatagta acaattggca atataaattt taattagttt ataaaataaa aatttaaaat
   114781 atttaatatt taaaatatct aatatttaaa ataaaataca aaaatttgtt tttatttatt
   114841 ttattattaa attttctgtt tatttaatca caaatattac aatattaaga ttttttcgat
   114901 aaatatttat ttaaatcgat ttttagattg ttttttaat
//
LOCUS       DAYAOY010000002        86989 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYAOY010000002 DAYAOY010000000
VERSION     DAYAOY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 86989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.002"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..522
                     /locus_tag="ACGI6B_000148"
     CDS             <1..522
                     /locus_tag="ACGI6B_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HFG9749623.1"
                     /translation="ADIKNKDICLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKN
                     NIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNM
                     IDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNE
                     SNRFDTESYGNNS"
     gene            476..904
                     /gene="ytfJ"
                     /locus_tag="ACGI6B_000149"
     CDS             476..904
                     /gene="ytfJ"
                     /locus_tag="ACGI6B_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HFG9749624.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1152..1592
                     /locus_tag="ACGI6B_000150"
     CDS             1152..1592
                     /locus_tag="ACGI6B_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9749625.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            1608..3059
                     /locus_tag="ACGI6B_000151"
     CDS             1608..3059
                     /locus_tag="ACGI6B_000151"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFG9749626.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3150..3818)
                     /locus_tag="ACGI6B_000152"
     CDS             complement(3150..3818)
                     /locus_tag="ACGI6B_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HFG9749627.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            4087..4227
                     /locus_tag="ACGI6B_000153"
     CDS             4087..4227
                     /locus_tag="ACGI6B_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749628.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(4402..4722)
                     /locus_tag="ACGI6B_000154"
     CDS             complement(4402..4722)
                     /locus_tag="ACGI6B_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFG9749629.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(4842..5579)
                     /locus_tag="ACGI6B_000155"
     CDS             complement(4842..5579)
                     /locus_tag="ACGI6B_000155"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFG9749630.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            5794..7617
                     /gene="acd"
                     /locus_tag="ACGI6B_000156"
     CDS             5794..7617
                     /gene="acd"
                     /locus_tag="ACGI6B_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFG9749631.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            7832..12130
                     /locus_tag="ACGI6B_000157"
     CDS             7832..12130
                     /locus_tag="ACGI6B_000157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFG9749632.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            12334..12795
                     /gene="rimP"
                     /locus_tag="ACGI6B_000158"
     CDS             12334..12795
                     /gene="rimP"
                     /locus_tag="ACGI6B_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFG9749633.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            12825..13979
                     /gene="nusA"
                     /locus_tag="ACGI6B_000159"
     CDS             12825..13979
                     /gene="nusA"
                     /locus_tag="ACGI6B_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFG9749634.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            13986..14267
                     /locus_tag="ACGI6B_000160"
     CDS             13986..14267
                     /locus_tag="ACGI6B_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFG9749635.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            14257..14568
                     /locus_tag="ACGI6B_000161"
     CDS             14257..14568
                     /locus_tag="ACGI6B_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HFG9749636.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            14590..16530
                     /gene="infB"
                     /locus_tag="ACGI6B_000162"
     CDS             14590..16530
                     /gene="infB"
                     /locus_tag="ACGI6B_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFG9749637.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            16573..16953
                     /gene="rbfA"
                     /locus_tag="ACGI6B_000163"
     CDS             16573..16953
                     /gene="rbfA"
                     /locus_tag="ACGI6B_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFG9749638.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            16934..17917
                     /locus_tag="ACGI6B_000164"
     CDS             16934..17917
                     /locus_tag="ACGI6B_000164"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFG9749639.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            17930..18835
                     /gene="truB"
                     /locus_tag="ACGI6B_000165"
     CDS             17930..18835
                     /gene="truB"
                     /locus_tag="ACGI6B_000165"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFG9749640.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            18966..19895
                     /locus_tag="ACGI6B_000166"
     CDS             18966..19895
                     /locus_tag="ACGI6B_000166"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFG9749641.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            19999..20256
                     /gene="rpsO"
                     /locus_tag="ACGI6B_000167"
     CDS             19999..20256
                     /gene="rpsO"
                     /locus_tag="ACGI6B_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFG9749642.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            20496..21341
                     /locus_tag="ACGI6B_000168"
     CDS             20496..21341
                     /locus_tag="ACGI6B_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9749643.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            21466..23577
                     /gene="pnp"
                     /locus_tag="ACGI6B_000169"
     CDS             21466..23577
                     /gene="pnp"
                     /locus_tag="ACGI6B_000169"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFG9749644.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            23843..24586
                     /locus_tag="ACGI6B_000170"
     CDS             23843..24586
                     /locus_tag="ACGI6B_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9749645.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            24744..25991
                     /locus_tag="ACGI6B_000171"
     CDS             24744..25991
                     /locus_tag="ACGI6B_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9749646.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            26026..26271
                     /locus_tag="ACGI6B_000172"
     CDS             26026..26271
                     /locus_tag="ACGI6B_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFG9749647.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            26281..27504
                     /gene="dapG"
                     /locus_tag="ACGI6B_000173"
     CDS             26281..27504
                     /gene="dapG"
                     /locus_tag="ACGI6B_000173"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFG9749648.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            27617..28351
                     /locus_tag="ACGI6B_000174"
     CDS             27617..28351
                     /locus_tag="ACGI6B_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFG9749649.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            28474..30885
                     /locus_tag="ACGI6B_000175"
     CDS             28474..30885
                     /locus_tag="ACGI6B_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFG9749650.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            30888..31946
                     /gene="mnmH"
                     /locus_tag="ACGI6B_000176"
     CDS             30888..31946
                     /gene="mnmH"
                     /locus_tag="ACGI6B_000176"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFG9749651.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            31940..33274
                     /gene="rimO"
                     /locus_tag="ACGI6B_000177"
     CDS             31940..33274
                     /gene="rimO"
                     /locus_tag="ACGI6B_000177"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFG9749652.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            33261..33803
                     /gene="pgsA"
                     /locus_tag="ACGI6B_000178"
     CDS             33261..33803
                     /gene="pgsA"
                     /locus_tag="ACGI6B_000178"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFG9749653.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            34091..35137
                     /gene="recA"
                     /locus_tag="ACGI6B_000179"
     CDS             34091..35137
                     /gene="recA"
                     /locus_tag="ACGI6B_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFG9749654.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            35313..36854
                     /gene="rny"
                     /locus_tag="ACGI6B_000180"
     CDS             35313..36854
                     /gene="rny"
                     /locus_tag="ACGI6B_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFG9749655.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            37168..39504
                     /locus_tag="ACGI6B_000181"
     CDS             37168..39504
                     /locus_tag="ACGI6B_000181"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9749656.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            39886..41667
                     /locus_tag="ACGI6B_000182"
     CDS             39886..41667
                     /locus_tag="ACGI6B_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFG9749657.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(41749..42279)
                     /gene="lepB"
                     /locus_tag="ACGI6B_000183"
     CDS             complement(41749..42279)
                     /gene="lepB"
                     /locus_tag="ACGI6B_000183"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9749658.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(42319..43143)
                     /locus_tag="ACGI6B_000184"
     CDS             complement(42319..43143)
                     /locus_tag="ACGI6B_000184"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFG9749659.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            43400..44308
                     /locus_tag="ACGI6B_000185"
     CDS             43400..44308
                     /locus_tag="ACGI6B_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9749660.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            44452..45897
                     /gene="purB"
                     /locus_tag="ACGI6B_000186"
     CDS             44452..45897
                     /gene="purB"
                     /locus_tag="ACGI6B_000186"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFG9749661.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            46208..46660
                     /locus_tag="ACGI6B_000187"
     CDS             46208..46660
                     /locus_tag="ACGI6B_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9749662.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(46995..48191)
                     /locus_tag="ACGI6B_000188"
     CDS             complement(46995..48191)
                     /locus_tag="ACGI6B_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9749663.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            48576..49526
                     /gene="galT"
                     /locus_tag="ACGI6B_000189"
     CDS             48576..49526
                     /gene="galT"
                     /locus_tag="ACGI6B_000189"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFG9749664.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            49687..50439
                     /locus_tag="ACGI6B_000190"
     CDS             49687..50439
                     /locus_tag="ACGI6B_000190"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFG9749665.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(50531..51307)
                     /locus_tag="ACGI6B_000191"
     CDS             complement(50531..51307)
                     /locus_tag="ACGI6B_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9749666.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            51622..53520
                     /locus_tag="ACGI6B_000192"
     CDS             51622..53520
                     /locus_tag="ACGI6B_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HFG9749667.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(53648..54769)
                     /locus_tag="ACGI6B_000193"
     CDS             complement(53648..54769)
                     /locus_tag="ACGI6B_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9749668.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            54968..55300
                     /locus_tag="ACGI6B_000194"
     CDS             54968..55300
                     /locus_tag="ACGI6B_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9749669.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            55287..56345
                     /locus_tag="ACGI6B_000195"
     CDS             55287..56345
                     /locus_tag="ACGI6B_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFG9749670.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            56338..57408
                     /locus_tag="ACGI6B_000196"
     CDS             56338..57408
                     /locus_tag="ACGI6B_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFG9749671.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            57510..58046
                     /locus_tag="ACGI6B_000197"
     CDS             57510..58046
                     /locus_tag="ACGI6B_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9749672.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            58162..58869
                     /locus_tag="ACGI6B_000198"
     CDS             58162..58869
                     /locus_tag="ACGI6B_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9749673.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            58871..59587
                     /locus_tag="ACGI6B_000199"
     CDS             58871..59587
                     /locus_tag="ACGI6B_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFG9749674.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            59589..60347
                     /locus_tag="ACGI6B_000200"
     CDS             59589..60347
                     /locus_tag="ACGI6B_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFG9749675.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            60482..61870
                     /locus_tag="ACGI6B_000201"
     CDS             60482..61870
                     /locus_tag="ACGI6B_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HFG9749676.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(61946..62767)
                     /locus_tag="ACGI6B_000202"
     CDS             complement(61946..62767)
                     /locus_tag="ACGI6B_000202"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFG9749677.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            63018..64172
                     /locus_tag="ACGI6B_000203"
     CDS             63018..64172
                     /locus_tag="ACGI6B_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HFG9749678.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            64355..64555
                     /locus_tag="ACGI6B_000204"
     CDS             64355..64555
                     /locus_tag="ACGI6B_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9749679.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            64976..65404
                     /locus_tag="ACGI6B_000205"
     CDS             64976..65404
                     /locus_tag="ACGI6B_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFG9749680.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(65547..66302)
                     /locus_tag="ACGI6B_000206"
     CDS             complement(65547..66302)
                     /locus_tag="ACGI6B_000206"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9749681.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(67003..67509)
                     /locus_tag="ACGI6B_000207"
     CDS             complement(67003..67509)
                     /locus_tag="ACGI6B_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9749682.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            complement(67591..67911)
                     /locus_tag="ACGI6B_000208"
     CDS             complement(67591..67911)
                     /locus_tag="ACGI6B_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9749683.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            68080..68277
                     /locus_tag="ACGI6B_000209"
     CDS             68080..68277
                     /locus_tag="ACGI6B_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749684.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            68382..68822
                     /locus_tag="ACGI6B_000210"
     CDS             68382..68822
                     /locus_tag="ACGI6B_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749685.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            69071..69514
                     /locus_tag="ACGI6B_000211"
     CDS             69071..69514
                     /locus_tag="ACGI6B_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9749686.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            69519..70583
                     /locus_tag="ACGI6B_000212"
     CDS             69519..70583
                     /locus_tag="ACGI6B_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9749687.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            70597..71025
                     /locus_tag="ACGI6B_000213"
     CDS             70597..71025
                     /locus_tag="ACGI6B_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9749688.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            71151..71597
                     /locus_tag="ACGI6B_000214"
     CDS             71151..71597
                     /locus_tag="ACGI6B_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9749689.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            71624..71791
                     /locus_tag="ACGI6B_000215"
     CDS             71624..71791
                     /locus_tag="ACGI6B_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749690.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            71791..74244
                     /locus_tag="ACGI6B_000216"
     CDS             71791..74244
                     /locus_tag="ACGI6B_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749691.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            74244..74666
                     /locus_tag="ACGI6B_000217"
     CDS             74244..74666
                     /locus_tag="ACGI6B_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFG9749692.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            74948..76477
                     /locus_tag="ACGI6B_000218"
     CDS             74948..76477
                     /locus_tag="ACGI6B_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9749693.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            76493..76819
                     /locus_tag="ACGI6B_000219"
     CDS             76493..76819
                     /locus_tag="ACGI6B_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9749694.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            76819..77247
                     /locus_tag="ACGI6B_000220"
     CDS             76819..77247
                     /locus_tag="ACGI6B_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9749695.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            77240..78292
                     /locus_tag="ACGI6B_000221"
     CDS             77240..78292
                     /locus_tag="ACGI6B_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9749696.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            78289..78987
                     /locus_tag="ACGI6B_000222"
     CDS             78289..78987
                     /locus_tag="ACGI6B_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9749697.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            78988..79971
                     /locus_tag="ACGI6B_000223"
     CDS             78988..79971
                     /locus_tag="ACGI6B_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9749698.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            79988..85309
                     /locus_tag="ACGI6B_000224"
     CDS             79988..85309
                     /locus_tag="ACGI6B_000224"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749699.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            85326..85679
                     /locus_tag="ACGI6B_000225"
     CDS             85326..85679
                     /locus_tag="ACGI6B_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HFG9749700.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            85942..86202
                     /locus_tag="ACGI6B_000226"
     CDS             85942..86202
                     /locus_tag="ACGI6B_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749701.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(86423..86878)
                     /locus_tag="ACGI6B_000227"
     CDS             complement(86423..86878)
                     /locus_tag="ACGI6B_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749702.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILLNRQ"
BASE COUNT        34918 a         9383 c        15227 g        27461 t
ORIGIN      
        1 gctgatataa aaaacaaaga tatttgttta aagcttaata taaaatacat gtttaattta
       61 ataaatataa atagacaact ttatccagct gaaaatagta aaaataatga taaaaaagag
      121 gggatgaaga ataatataga ttcaagtata ttattagctg atgacctttt gagcatttat
      181 agattattaa aaaagataaa aatacatgaa ctttattcaa atattaattt tggaactgga
      241 aatattggct taacatcttc tgtatatgta ttgataaata ctttgtatgg aaatttattt
      301 aatatgattg atgcagaaaa aatgtattta aatgtaaatc cagatttcac aaaagattat
      361 gttttaggaa atataaggat acatataagg ccaagaataa aagctttatt taatattata
      421 ataatgatta ataaaattat gaataaaaat aaaggaaata aggaaggtga tagtaatgag
      481 agcaacaggt tcgatacaga gtcttatgga aacaactctt gaaacaatta aaggttctat
      541 agatgcaaat actattatag gagaccctat aaaaacagat actacagtgg ttgtacctat
      601 atcaaaggtt acaattggct ttggtatagg aggaggagaa tactcaaaag gatatgatga
      661 taaagatagg gaaattgaat taaaaaatga aaaatcagat acgaattttg ctggaggtag
      721 cgcaggggct atatctgtac aaccagtagc atttgtagta gttgaaagtg gagagactag
      781 aataatgagt ttagatagta atatcaatct agtggataat atattatcta taactcctag
      841 agtattggag aaaattcaaa atatatcaca aagtaataag aatcaagata aaaacaatat
      901 gtaagaataa aatattttta aatatgtata tagttttaag taaagtagtg gggttaatat
      961 aaatcccact attttgttgt gtactttaaa catttatatc taataagatt ttagtttaaa
     1021 acaatttaaa tagataataa agttgatata tagtataatt ataaaaagaa aagaagtatt
     1081 atattataga tatataaacg catttgagat ataagaggga gttataaatc attagttttg
     1141 aggtgaatgt tatgagcgat aaagaattat ctattttgtt gttagaaagt atgaataatt
     1201 ttcataattt aataaagatt attaataacg aaagatataa aaaagataag gttcttacag
     1261 aaagacagtt ctttgcctta gtaaaaataa gaaaacatga taaaatagag cttaaaaatc
     1321 taagtagaga tttgcatgta tctacttcta gtctgtgtat actacttaat aaattggttg
     1381 agcaagagta tgtatatagg gaagaagata gtagagatag acgaaatact ttttatggaa
     1441 taactaaaaa tggagaaaag atattggata atgaaatact aaagtttgta tctataataa
     1501 gtgataaaat ggattgccta gatataaata ataaagataa attatttact tctttggaag
     1561 agtctaaaaa tataatagaa caattatttt gaaaaggaga atttattatg aatataaagt
     1621 attctaaaga aatattaaaa agtttagaaa atgctataac agtagtagct ctatttgagg
     1681 gagaagatgt ttctacgaca tttgataaaa taaatatgat aagcagaaat tttaattcta
     1741 aagaaaatag tatagatact gtaacattgg ttgaaaataa caatttggta aatgtttgtc
     1801 ttgttggttt tggtaagaga gagttattga atagagaaaa aattagaatc ataggtggga
     1861 atttgtgtaa aaaattaaaa aatatattga aaaatgaagc atataagagt agtgatgtta
     1921 atgtactgaa tcttaattta aatagtgatg aaataggttg ttttgtagaa gggatattac
     1981 ttggaaatta taagtttgat aaatataaga caaaaagtaa ggattttgaa agaaaagaag
     2041 ttgatactat aactatattt actgaaaaag aagttgagtg tcaaatagaa aaggctaaaa
     2101 tattagctaa ttcaactata atagctagaa atctagttaa tgaaccatca aacgtaatat
     2161 atccaaaaac attagcagta gaaacagtta aacttggtgc agagtttggg tttaatgttg
     2221 atgtttatga agaagaaaaa ataaaggcat tgggaatgga tgcttttttc gctgtttcaa
     2281 gaggttcaat taataaacca agattcatag taatgagata ctttggagat aaagaaagta
     2341 cagatatatt aggtctagta ggtaaaggat tgacttatga tactggtgga tattctttaa
     2401 aatcaaatgc ttctatgcta gatatgaaga cagatatggc tggtgctgcg agtgttatag
     2461 gtgctatgtg tgctatatct caaagtaaat taaagaaaaa tgttatagca gtagtggcag
     2521 catgtgagaa tgctttatca ggaggttcat acaaacctgg agatataatt agttctatgg
     2581 caaagaaaac tattgaagtt ttaaatacgg atgcagaagg tagacttaca ttagctgatg
     2641 caatatacta cattataaac aatgaaaaag taacaaaggt ggttgatgta gctacattaa
     2701 ctggtgctgc tttaatattg ttaggtaatg tcgcaactcc tatagtaaca aataatgatg
     2761 atttttattg tgaattagaa aaagctgcca ctttatcagg ggaaagggta tggaagatgc
     2821 caatttatga tgaatttaaa gatatgataa aaggagaaga agcagacctt aaaaacactg
     2881 gtggtaaaaa tgctggatgt ataacagcag gagcttttat tggtgaattt gttgggaata
     2941 ctccttggat acatatggat atagcgggga caagtacatc atcaaaatca attggataca
     3001 aagcaaaggg ggctacagga gaacctgtta gaacattgta ctatttggca gaaatctaaa
     3061 aaacaattat aaaatataat ttataaagcc acgtggatat ttttgtaatt taagattatc
     3121 tatgtggctt tattttttat ttaaataaat tatttactct ttttcttttt ttcaagtata
     3181 aaagtatctc tattaaataa aagagcaggt atgtacataa tactagtaac aaaaataaac
     3241 gtaaaaatag cagttgtgct atgacttaaa aaaccataat agtacctaga gttggtaaat
     3301 gtgtttagta aaaatatgaa tgaaaaataa ataaaacata tagctaagtt atctctaagt
     3361 gcatatctgt taactctaaa ttttctaaca tcaaccatat atatatctgc ataaattatc
     3421 aagcacttag agaagataaa gataaaatat attaagctag gaaattcata gcccaagttt
     3481 ttagaaaaaa tgagttctcc aactgaacaa actaaactcc aagaaaaaaa tatattaaaa
     3541 agatgatact gcttaaaaat tagtatggct atacacagat atatacaaat ataattgatt
     3601 ccaattggta aagagtttag agtactataa ttacctgaag aaataagtaa taattgctct
     3661 agagccataa ttattacaag taatgtacaa attatcttct caactgtata gctataaggt
     3721 aaaatatttt tggttagttt tggacaatag tataaaaata atccaaatat aaagagactt
     3781 acaaattgtt cactagaaaa aagaaaaatt ttaaacatat aacattctct cctataataa
     3841 atttaatatg tatctatata aatatattca ttatatctca atttaatata taaaaaagat
     3901 aataatagtt agatttttta tatgaatttt aataggtaaa atatcctatt gttttgtaga
     3961 tatatcctaa ttatgtcaaa aaataaaaaa atttttattt attagagttt ttggaaataa
     4021 taataacaga tattataatt aattaacagt ttttgtaagt atttgacaat agagagggtg
     4081 taacaaatgg gaataataga gtcagcatca aaattggctg agatggttca tttattggct
     4141 gttgaaaagg ggataactga tatagaagca tgggatgaag ctgtgaaaga atacagcaaa
     4201 atttatgaag aaagaagaaa tgaatagaat gataaaaaat gaaaacttga tgataaaaaa
     4261 taactttatt taaagaaatt caatttttat agaagtatta aaaataatat aaataaaaag
     4321 atggtatttc aaatgtaagt aaaaaatcat tttgggatac catccttatt atttatttta
     4381 ataatatata agtaataaat cttacttatc catatttttt atgtaacctc ttttttctaa
     4441 cttttttata tgctccttac gtatttcatc aagattaata ccatattgtt cttctagctt
     4501 aaacatcata gtaacacatg tctgagctac atccattagt tccttagcag ttttctttat
     4561 aatatcaact tcatctaaat cattattttc tccacttaga tttctgaatt tacctataca
     4621 ttcagctaat tcaccttgtt cttcacccat tttgataaat gtagattcta gtgttgggtc
     4681 taaattatta agctctggta aagatatagt ttttagtttc ataaaagctc ctttcaagtt
     4741 attcgtattt atactaaaac atcataacaa aaataatact aaaaaaaaac tttaaattca
     4801 acataaaaac ttacatatat aataaaaatc gtgaaataaa tctatatttg tcttaataca
     4861 gcctcatata aaacttctcc aataggttta tttattacaa gagaagctga cttatcatag
     4921 actgtatttg ctttattaat aagagcaata taatcacctt tgtaataatc tataaaactt
     4981 gctgctgggt acacagctaa tgatgtacct cctataatca ataaatcagc attacttata
     5041 gcagaaattg ttttagtaat gacatcactg tcaagagctt cctcataaag tactacatct
     5101 ggttttacta tagaaccaca gttgtcacag taaggaatat tacctcccag atttaacata
     5161 gattctaagt caaaaaactt accacattta gtacaataat ttctatgtac agaaccatga
     5221 agttctagca catttttact tccagccatt tgatgaagtc catcaatatt ttgagttatg
     5281 acagctttaa gcttacccat ttcttcgagt tttgctaaag ctatatgagc attattgggt
     5341 tttgcattag gatatattaa tttatcctta taaaattcaa aaaattcttc tggatatcta
     5401 acaaaaaaag tatgtgaaac taattgttca gcagtaaatt gtttattgag tttttggcta
     5461 aataatccag tagaacttct aaagtctgga atattagatt cagttgaaac acctgctcca
     5521 ccaaaaaata caatattatt gtgattagct attaagtctt ttaagctatt tgcatccata
     5581 gttacacctc caaattatta tatataaatt gtactacata ttaggtcgtt tatgttttat
     5641 taatttaaat aaatatatag tttaataagt taaataaagg gaatataaat aaaatgtata
     5701 cagagtataa atagacaata tttactaaaa taacaagttg tcgtataatt atattaaaat
     5761 tgttttagtg tataagaagg gcgggggaag tacgtgaaga aaaaagcagc tttagcaaca
     5821 ttggctatgt tacccttagg tgtggtaaat gcacatgctg atggagatat aggtatagtg
     5881 actataaatt atttaaatgt aagaaatgaa ccaactgccg aaagtagcat agcctttgtt
     5941 gctaaaaaag atgataaagt tttgattaaa gactcttcta atggatggta taaaataaaa
     6001 gctgaatctg gacaagaagg ttgggcttca tcaaaatata tagcaaaatc aaatagtgac
     6061 tctttaagaa cgtctacaaa taaagaaaaa caagttattt caaatagttt aaatatgaga
     6121 aatggagcag gtacaagtta cagagtaata actgtactaa aaaaaggtca aaaagtagaa
     6181 gtaatatcag agagtaatgg atggtctaaa atcaaatatg atggaagact aggatatgta
     6241 tctagctctt atttaggaga cgtttcaaac tcaactaata aatcaaaaac taaacaagtg
     6301 aatacaactt cactaaatgt aaggagtgga ccaaatacaa gttatggttt gttaggcaag
     6361 ttgccaaaag gaagtaaagt agaagtaata tcagaaggta atggatggtc caaaataaag
     6421 tataatggaa aagatgccta tgtatctagt atgtacttat cggatgtaag tcaaagtaat
     6481 tcagacaatt ctagtcaaag caatgataaa aagaatactg ataaggtcgt aaatacagct
     6541 tctttaaatg taagaagtgg accaggttct acatatagta agttgggaaa agtttataaa
     6601 ggaagtaaag taactgtact atcagaaagt agtggatggg ctaagattaa ttttaacaat
     6661 aaagaagcat ttgtagtagg caattattta tctacttcag cagatacttc aaataataac
     6721 tcaaatagta actctgataa cagttctaat agtaatggca ataattcttc atcatcaggt
     6781 caagtaaatg gtatgtcagg tataagtggg gctaaaattg attataaatc tttgagttat
     6841 actttagaat ctcatataag taaacaagtt gagaaagcag catcaggagg aaatgtgata
     6901 gctccaagta ataggaaaag cactccaagt cctgaattta gtactttttc agctcaaaga
     6961 acaagctcat ttgtaaatgc aagttcaagt gatatagaat attatttaaa tcctaaaaat
     7021 tttacaaata ctactaaagg tatgatgcag tttttaaaga ttaacagtta tagagatggt
     7081 atttcagaat caagtcttaa ctcgtatctt aatggtttat cttctagtgt atttaaaaac
     7141 cagggagcag cctttataaa tgctgccaag aagtataata tagatgttgt atatctagta
     7201 tctcatgcta tgtgggaaac tgcttatggt aagtctacac ttgcccaagg tcaaacatta
     7261 acatcttata agggacaacc tcttagtaag ccagttaaag tatacaattt ttttggtata
     7321 ggagcaatag ataaaagtgc taatgtttca ggtgcagaag cagcgtattc caatggctgg
     7381 actagtgtag aagctacaat agatggctct gcaaagtgga tatcacaaaa ttacgtaaat
     7441 agttctaagt acaatcagaa tactatctac aagatgaagt ggaattatga ttatacatgg
     7501 catcagtatg caactgatgt gaactgggct aatggaattt ctggaattat ggaaaattta
     7561 attggtcttt atggaggagg aagtagtttg gtttttgaag ttcctcaata caagtaatac
     7621 ataaataaaa aatatagact atgggtagtt ttcattttat ttaaattatc tatagtcttt
     7681 ttgtatataa tttataatct gtaaatttaa tgctaacaaa acttttatgt tataatatta
     7741 attctaagta aatataagtg agtaataggt agaaatagat aaaaagtggt aataatatat
     7801 ataaagataa ttttgagaaa ggaggatata tatggaaagt attaaggaat atttggataa
     7861 gttagaaatt aataatagtg gtcttggaaa gcaacttaaa gaagtttata taaatagagt
     7921 agtttatttt aaggaagata agatagttta tttttatcta acttctaaag atattgtttc
     7981 tcatgaactt ttagataaat ttaaagaaga attgatgtat aaactcgatt attttaaaga
     8041 tatgaggatg aagataagat ttactggtct tgaaagaaaa tccaataaag atgttataaa
     8101 aaaatattgg aataatatat tgtatatatt aaagtatctt tgtccatcta ttgcagggtg
     8161 gtataaacaa gtagaatttc tttgtttaga agaagaatta aaaataaaac ttccaaaagg
     8221 tatattttat gagcgattaa tgaaaaaaaa tgtaacttat gtacttaaaa ccgtgcttag
     8281 cgaagagttg ggattagatt taaatataac aatagaaaaa gctgttgatg aaaaggtaaa
     8341 taaagaaaga ctcataagaa tcaatgatag agaaatggaa gaaaaaatta gggctttaga
     8401 aattggaaaa gttaataact gtgaagaaaa tgaagaggaa agttatgtca ttaagtctga
     8461 tgtagatgaa aatttaatct atggagataa tgcaaatgct atgattgaaa atatagttga
     8521 attaaatgca tcttctggaa cagttgcagt tgttggagat atatttgatg ttgacactaa
     8581 agaacttaaa aacggaaaaa tacttatgat agcttcaatt acagattata ctagctcaat
     8641 aagctgtaaa ctttttttaa cagatacaaa taaggatgga gtacttgaaa atgttaaaaa
     8701 aggagcatac ttaaaaatca agggtgacat agtttatgat acatatcaaa gagaaattag
     8761 tatgatgatt agtgggataa gaaaagaaac tagaatagag agagaagata aatcagagga
     8821 aaaaagagta gaactccatg cacatacaca aatgtcttct atggatgcta tttgttctgt
     8881 taaaaagctt gtagagcgtg cagccaaatg gggtcattct gctattgcta taacagacca
     8941 tggtgttgta caaggatttc cagatgctat gaatgcaggt aagtcaaata atataaaaat
     9001 actttatgga gtagaagggt acttggttga agatgattct ctcattatag aggatgcaaa
     9061 tgataaagaa ctttcgcaga catttgttgt atttgacata gagacaactg gtttttctaa
     9121 tacaaatgat aaaataactg agattggtgc tgtaaaaata gaaaacttta agattgtaga
     9181 tagatttagt gagttaataa atcctgaaaa agatatttct tataagatac aggaattgac
     9241 aggaataact aatgaattaa ttaaagacaa gccaaccatt gaagaagttc ttcctaagtt
     9301 tatggaattt gttggagata gtgtattagt agcacataat gcagagtttg atactggatt
     9361 tatatcgcaa aaatgtaggg aacaaggctt gttatataat aacaaaaaag tagatacact
     9421 tatgttagca agagtaatgt tgccaaactt aaaaagacat agattaaatg tcgttgcaaa
     9481 agaacttggt attcctcttt taaatcacca tagagcagtg gatgatgctg aagctacagc
     9541 acttatattt aataagtttt tgcagatgct tacagaaaaa ggtgcaaaaa ctctaagtga
     9601 tgtaaacaat atacttggaa agattgatta cacaaagctt agcacaaatc atataacctt
     9661 gatagcaaaa aattctatag gtataaaaaa tttatataaa ataatttcag atgcacatgt
     9721 aaatcatttt tttagagcac caagaatcct tagaagtgta ttaaatgaat ataaagaagg
     9781 attaataatt ggttcggctt gtgaagctgg ggtggttttt caagctgtaa agaaaaacgt
     9841 gtctgatgag gaaatgaaaa aaataataga tttatacgat tatattgaag taatgcctat
     9901 agataataat agatttatga ttgataaagg agaagtaaag gacgaagaag agttaaggga
     9961 gttgaatagg aaattaattg atacagccaa aaaatttgac aaaattccag tagctactgg
    10021 ggatgttcat tttttagaca aacatgaagc tgtcttaaga aaagttctta aatattctca
    10081 aggttttaaa gtagatgaag aagaaactta tttacatttt agaacaacag atgaaatgtt
    10141 ggaagagttt agttatcttg gagaagaatt ggcatatgaa gttgtagttg aaaatagtaa
    10201 tctgattgcc gatatggttg aagatataaa acctatacca gatgatactt atcctccaat
    10261 tatagagggc tcagatgtgg agttaagaga aatgtgttac aaaaaagcca aacgaatcta
    10321 tggagaccca ataccagaaa tagttcaaaa aagactggat agagaattaa attcaataat
    10381 aagcaatggg tatgcagtta tgtatattat agctcaaaaa ctggttgcaa aatctctttc
    10441 agatggatat ttagttggtt caagaggctc agttggctca tcttttgcag ctactatgag
    10501 tgatataaca gaagttaatc ctcttccagc acattatatt tgtgaaaacg aagactgtaa
    10561 atattcattc ttttatgaaa taggtgaatg gggctcaggt gttgatttac cagacaagga
    10621 ttgtccaaag tgtggaagaa aacttgctaa aaacggtcat gatattccat ttgaggtttt
    10681 cttaggtttt gaaggtgata aagaaccaga tatagactta aatttctcag gaaattatca
    10741 gccaacaatt cataaataca cagaagaact atttggagag gggtatgtat atagggctgg
    10801 gactataggt actgtagcag aaaaaacagc ttttggatac gcaagaaaat atgtggaaga
    10861 aaataatata agtgtaccaa atgcagaagt acttaggctt tcaaatggat gtactggagt
    10921 aaaaagaacc tcaggacaac atcctggtgg agttatggtt ataccagact ataaagatgt
    10981 atacgatttt acacctatac agtatccagc aaatgatact tcttgtggtg ttataactac
    11041 tcactttgat tatcattcaa taagtggtag aatacttaaa cttgatatac ttggacacga
    11101 cggtcctacc attataagga tgcttgaaga tatgactgga ataaatataa cagaaattcc
    11161 tttagatgat aaagagacta tgtctctatt tacatctaca gaagcattag gtgttacacc
    11221 agaagaaata aactgtccta taggttgcct tgctgtacca gagtttggaa ctaagtttgt
    11281 acgacaaatg ttacttgaca caaaacctac tacatttgca gaacttgtac gtatatctgg
    11341 tctctctcat gggacagatg tttgggtaaa taatgcacaa gacttagtta gagaagatat
    11401 agttggatta aaagatgtta tatctacacg agatgacata atgaactatt taatatttaa
    11461 aggattacct cccaaaatga gctttacaat aatggagagt gtaagaaaag gtaaaggttt
    11521 aaaaccagaa cacattgaag aaatgaaaaa aaataatgtt ccagaatggt atataggctc
    11581 atgtaaaaag attaagtaca tgttcccaaa agcacatgct gttgcttatg taatgacatc
    11641 atttagaatt gcatattgta aagttcacta tccagaagca ttttatgcga cttattttac
    11701 gacaaaagtt gaggattttg atgcagattt aattgttaaa ggattagatg ctataaaatc
    11761 aaaaataaat gaaattgagt cactgggaaa tgatgcaacc acaaaagaaa aaggtatgtt
    11821 gaccgtttta gaagttgctc ttgaaatgta tgctagagga ataaaattat taccagttga
    11881 tatatataaa tcagatgcaa ctgagtttat agttgtagga gaaaaaacat tacttccacc
    11941 aatggcagca atacaaggtc ttggaggaaa tgcggctata aacatccaaa atgaaagaaa
    12001 aaatggtgaa tttatatcaa aagaagattt gagaaagaga acaaaaatat caaaaacggt
    12061 aatagaaact cttactaatc atggttcact tgagaatatg agtgagagaa atcaattgtc
    12121 attgttttaa gttagcccaa aaagttgcaa ataaaagaat tttatgatat catagtatag
    12181 aaaatagtat actttgcaga aactaagagt gagacagctg ttctcactct tttattttaa
    12241 atatattatt aaaaagctgg tttattttta ttaaatatat tttatttaag attatataaa
    12301 ctggtcattt acaattgaat aggaggaact ttaatgaaaa aaaatttaga agctacaatt
    12361 gaggagattg taactaaaat aacggatgaa catggatttg aaatggttga tgttgaatat
    12421 gtaaaagaag caggagaata ctacttaaga gtatacatag ataaagaaga aggaatatct
    12481 ttaaatgaat gtgaattagt tagtagagaa ttaagtccaa tactagatga aaaagaccct
    12541 ataaaagaaa attacttttt agaggtatct tctccagggc ttgatagagc attaaaaaaa
    12601 gacagagatt ttgttagata tcaaggtaga gatgtagatt taaaattata taaaccatta
    12661 aatggatgta agcaatttga aggtgaactt gtaggattaa cagaagataa taatataaaa
    12721 atcattgcaa atggtaagga aatggaattt aatagaaaag atgttgctat tgtcagatta
    12781 gcaataaagt tttagtggta tattaaatag ggaggatata aaaaatgaat catgaattta
    12841 tggaagcatt agatgaatta gtagttgaca gaggtataga taaagaaata cttatagata
    12901 ctatagaaca agctctttta actgcataca agaaaaactt tggttcagca caaaatgtta
    12961 gagttgaatt tgatagagac aagggagata taaaagtatt ttctcaaaga gttgttgttg
    13021 atgaatcaga tttatatgat actttcttag aaatagagtt agcagatgct agagagataa
    13081 accctaatta tgaattaggt gatattatag aacatgaagt tacacctaaa aactttggaa
    13141 gaattgcagc tcaaacagca aagcaagtag ttgttcaaag aataagggaa gctgagagag
    13201 aaatagtgta taatgaattt atagaaaaag aaaatgaaat agttacagga gaggttgcta
    13261 gagcaaataa aaatgttgtt tatgtaaatc ttggaagaat agaaggtata atgactcaaa
    13321 gtgaacaaat accatttgag aattataaag caggtcaaaa aataaaattc tatatacttg
    13381 aagttaaaaa gactaacaaa ggacctcaaa tagttgtatc aagaagtcat ccgggtctag
    13441 taaagagatt gtttgaagaa gaagttcctg aaatatatga aggaatagtt caaataaaat
    13501 ctgtagcaag agaagcaggg tcaagaacta aaatggcagt aagatctata gatgaaaaaa
    13561 tagacccaat tggagcttgt gttggaccaa aaggacttag agtaaaaaat atagtggatg
    13621 aacttggcga tgagaaaata gatataataa aatatagtga agacccagca gagtttatat
    13681 cagcagcact tagcccatca aaggttgtta gagtagatat agatgaagat gaaaagtctg
    13741 ccttagtaat cgttccagat tatcagttat cgcttgctat aggtaaagaa ggtcaaaatg
    13801 caagactagc agcaaaattg actaactgga aaatagatat taagagcgaa tctcaagcag
    13861 aacaagaaat gttaaatctt gaaaattcca aagaaataga agctgaggat gttgaagctg
    13921 aatttatcga ccaagaagag ttagatactt cagaggataa ttttagtgaa gaagagtaag
    13981 gagggatgat tttgaaaaaa gtaaaaaaag tgcctcaaag aaaatgcata gcatgtcaag
    14041 atagagactc taaaaaagag cttataagaa tagttaaaaa taaagaagga aaaatatttt
    14101 tagacccaac aggaagagcc aatggaagag gagcatatgt atgtaaaagc tctgaatgtc
    14161 taaaaaaagc tataaagagt aaagcattaa ataaagcttt taaaatagat gttcctgatg
    14221 aagtctatga taatctactt atggagttag aagaatatga aaaataataa agaaaaagta
    14281 ctttcgttct tagggctcgc aactcgtgca gggaaaatag tgtctggtga tgattcaact
    14341 ttgcttgacc ttaaaaaagg aaaagtaaat ttgattttaa tcgcagaaga tgcatcaaat
    14401 aatactaaaa aactttttaa agataaatca acttttagaa atattccata tctatttttt
    14461 tcaacaaaag aggaaatagg atttgcaata ggaaaatctc caagagcagt tgtaggaata
    14521 aaagatgaaa atttttctaa aaagataatt gaactaatag aaatttaaaa taaaataggg
    14581 ggtgatcttg tgtcaaaaac aagagtatac caaatagcag aagaattaaa tatatcaaat
    14641 gaagagctta taaataagtt agctgaatta gatataaatg taactgacaa agattcagta
    14701 ttagaaggtg aagagttaga attagcactt gaaatgttag gtgaagattt atctcaagaa
    14761 aatggaaatg ttatagaaat agatggaaag ttgacagtac aagtattagc aacgaaatta
    14821 gataaatctc catcagaaat aattatgaag cttatgaaaa tgggaactat ggcaacaata
    14881 aatcaagaga taagttttga gattgctgct ttggctgcca aagactatgg atttgaatta
    14941 acagtagctg aaagtgatga tacagaagct ttagaaatag aagctttaat ggaaattgaa
    15001 gaagataaag aagaggattt aaaacctaga ccaccagttg ttacagttat gggacatgtt
    15061 gaccatggta agacatcttt actagatgca ataagaaaaa ctgatgtgat ttcaggtgaa
    15121 gctggaggaa taactcagca tataggtgct tcagaagtta aaatcaatgg acacaaaata
    15181 gtatttttag atactccagg acatgaggct ttcacatcaa tgagagcaag aggagcacaa
    15241 gttacagata tagcaatatt agtagttgca gcagatgatg gtataatgcc tcaaacagtt
    15301 gaagctataa accatgctaa agcagcaggt gtacctttaa tagttgctat taataaaata
    15361 gataaaccag gtgcaaaccc agataaggtt aaacaggaat tagcagacca aggattatta
    15421 gttgaagact ggggtggaga agtaatagca gttccagtat cagctaagaa aaaagaagga
    15481 atagatacac ttttagaaat ggtattattg gttgctgaaa tggaagagtt aagagctaat
    15541 ccaaataaaa gagcagtagg aactgttata gaagctgagc ttgataaagg aagagggcca
    15601 gttgcaacag tacttgttca aggtggtact ttaacagtag gtgacccaat cgttgcaggt
    15661 gttgcatgtg gtaaagttag agcaatgata aatgcaaaag gtaagagagt aaaaacagca
    15721 ggaccatcta cagcagtcga gatattaggt ctatcagaag ttccacaagg aggagaccaa
    15781 tttgtcgaag ttccaactga taagattgct agaagtgtag cagcaagaag acaacaaata
    15841 gttagagatg aaatgttaaa atcaactcaa agattatcac tagatgctct atttagtcaa
    15901 atgagtgaag gttctataaa agaccttaat atagttataa aagcagatgt acaaggttca
    15961 gtacaggcag ttaaacaatc tttagaaaaa ttatcaaatg aagaagttca agttaaagta
    16021 atacatggtg gtgttggagc tgtaacagaa tcagacatat tacttgcagc agcttctaac
    16081 gcaataataa taggattcaa tgtaagacca gttccaggtg cagaatctct aggagaaaaa
    16141 gaaaatgttg atataagaac atatactata atatacaagg caatagaaga tatacaagcg
    16201 gctatgactg gtatgttaga ccctgaatat gttgatgaag aaacaggaaa agctgaaata
    16261 agagaaatat ataaaatatc tggtgttgga acagtagctg gatgttatgt gactaatggt
    16321 aagatattta gaaattgtaa agtaagatta gttagagata gcataataat acatgaaggt
    16381 gagcttgcag cacttaagag atttaaagat gatgtaaaag aagttaattc tggatacgaa
    16441 tgtggtatga gttttgtaaa ttataacgac ataaaagaag gcgatatagt agaagcttat
    16501 ataactaaag aagtagaaag aaaattataa attcgtatca aagaaaatca gttagtaaag
    16561 aaggtcgatg gtatggcatc atataataga acaagaagga tagcagaaga aataagaaaa
    16621 gtcgtaagca ctatgcttat aaatggagta aaagacccta gaataacatc tatggtgagt
    16681 gtaactgatg tagaagttac aaatgaccta agatatgctt atgtatatgt aagcattcta
    16741 ggtggagatg aagaatccac actaacaggt cttaaaagtg ctggaggata tataagaaga
    16801 gaagtaggaa aaaatataaa acttagatat attcctgaaa tagtatttaa acttgatgat
    16861 tcaatagaaa aaggaatgta tatggatagc cttataaaaa gagttaatga aaaaaatgct
    16921 caacaaaatg aggatgaaaa ttatgatgaa taatatggat agtattataa actatattta
    16981 tgagggtgac tattttatag ttacctctca tatcagtcca gatggagaca atattggttc
    17041 tacactttca atgtattata cattgaagaa tttaggaaaa aatgtatatt atgtacttga
    17101 tgatgaagct ccattaaatt taaggttttt ggttgaagat gtaaaaatct ataagtctaa
    17161 tgaatttaat atgaagaact attctctaat tgcacttgat tgtggggata agatgagaat
    17221 atgtgtgagt gatgaaatca aaaataatgc atcaaaatta atctgtattg accatcatgc
    17281 tagtaatgat tcatatggag atttaaatta tgtagatatt gatgcatctt ctacatgtga
    17341 attagtttat aatttattag ttagatttca gcaaataaaa gatattaata ttataaatga
    17401 agatatagca acatgtttat atacaggtct tgtaactgat actggaaact tttcgtattc
    17461 aaatgtacat gcaagtagct ttgaaatggc aaaaaattta cttgttttag gtgcacaaaa
    17521 aaatactata atacaaaata tatatcaaag caattcttct ggttattaca aattattagg
    17581 agaagctcta aagggtttgg agatatttga ttcaaaggta tctagtatag ttttaactca
    17641 agacatgatg aatagaaata atattagctt taatgatgtt gatggaataa caacatatac
    17701 aagagacata aaaggtatag aagttggaat tttatttaaa gagaaaaaac aaaatgaaat
    17761 taaagtgagt tttaggtcaa aaaattatgt tgatgtaagt gaaatagcca agttatttgg
    17821 tggtggtggt catgtaaggg ctgctggatg tactattaga gatagtattg ataatgcaaa
    17881 gaaaatggta ctagaggcag tgttaaaatc aatttaaggt gacagtaaaa tgaataagat
    17941 tataagtata ttgaaaccca caggtatgac atctcatgat gttgtaagta gagtgagaaa
    18001 gattttaaat ataaaaaaag ttggtcatac aggtactctt gacccagatg cttcaggtgt
    18061 tttaccaatc tgtataggga aagctacaaa agtatgtgaa gttattttaa acaaagataa
    18121 atcgtatata tgtgagctta ctttaggtat aagtacagat acttatgatg cttcaggtga
    18181 aattttaaaa aaagttgatg attttaaatt tagtaatgaa gacatagaaa gagcttttga
    18241 cacacaaaga ggagagataa atcaacttcc tccaatttat tcagctttaa aggtaaatgg
    18301 aaagagaatg tgtgatttag taagaagtgg tagacaatct gaaattactt taaaaacaag
    18361 aagagtaaat ataaaagaca taaaaatact aagtataaaa ggtaataaag taatgtttta
    18421 tgttgagtgt tctaaaggga cttatgtgag aagtatttgc catgatatag gagaatatct
    18481 aggttgtgga gctcatatgt cgtttttgaa tagaacatct tctggtaaat ttgacttaga
    18541 taattcaata acattagaag aattagaatt attttatgaa aataaaactt tagacaaata
    18601 cttatatgat atagattatg tccttgattc gtttaattat gttgtattaa accctaatgc
    18661 tataaaatat tatagtaatg gtggctcaat agatgataaa aggtttttaa agaataattt
    18721 cgataaagat gatgaatttg taagagtata cagtacagat aatttcttag gattaggaaa
    18781 gttatcaaaa cacaataata caataagtgt taaaagtgat aagatgttca tataaccaat
    18841 gtatttaaaa aaacaacaaa agagatagta ctataataac ttctatagtc tatctctttt
    18901 acttatgcta taatagtttg tgaataagtt aagagtaaat tatattttag cggggaacat
    18961 ttattatggt gattataaaa tctataaaag aaatagcaaa cataaaagaa agtgtcatta
    19021 ccataggaaa ttttgatggt gtacacaaag gacatcaagt tcttatagga aaaacagttg
    19081 aacatgcaaa aaaagaaaat atacagagta ttgtatttac atttgcaaat catcctgtaa
    19141 attactttag accaaactca acaaaaaata ttataagcaa tgatgataaa ataaaattat
    19201 tagaagattt gggaatagat atagttgtaa tcataccatt tgatgagtat atgactaaaa
    19261 tacctgcaaa ggatttcgta gatgagatac tagtaaaaaa attaaaagct aaaaagatag
    19321 taattggaca tgactttaca tttgcaaggg caaaagaagg caatgttaat ctattaaaaa
    19381 gcttagaaca tgaatttggt tttgaggtag aagtaataaa acccatcaaa atcaatgatg
    19441 taagagtgag tagtacttat attagaagtt tagtatccca gggtgatatg gctaatgtca
    19501 aggaatattt gggtagaaat tacaagttag aaggttgtgt tatacattct aaacaccttg
    19561 gaagaactat aggattccca acggcaaata ttgaccttaa gaataatatg ctcgttccaa
    19621 agagaggtat ttatgcttct attgtacata ttggagatga gacttatttt ggtgctacaa
    19681 atgtaggcta taacccaaca gttaatggaa aaactttgtc tattgaaaca aatattttag
    19741 agtttgatag agatatttat ggggaaaata taattgttga atttttggaa agaataagag
    19801 atgaaagaaa gtttaattca atagatgact taaaaaatca attgtataat gatacaaatt
    19861 atgtgtatga aaactatgtt tgcaaaaaca agtaatgtat gataaaatga ttaaggaaaa
    19921 taaccattac tcggcatttg aatactccaa tcaatgctta gtaataggcg tttctaaaaa
    19981 aatacggagg taatctaaat gataaacaaa tcagaaataa taaaagaata tggaagagct
    20041 gaaggggata ctggttctcc agaagtacaa atagctttat taacagctag aataaacgag
    20101 ttaaatggac atttaaaaac tcataagaaa gaccaccatt caagaagagg actattaaaa
    20161 atggttggta gaagaagaaa cttattagct tacttaaagg aaaaagatct tgaaggatat
    20221 agagcattaa tagctaaatt aggattaaga aagtaatttc attctttata aaaaagcagg
    20281 tatagtgtat acctgctttt ttatagttaa ttcattataa agtaaaatag ttaataataa
    20341 aagtagttta tatgttataa aaaattataa aatgaataat attgggtaag aatattaaaa
    20401 tagtatacaa atacataatc aatttaaaag gaaagacagg ataaatatag aaattttata
    20461 acaggataat tagttggaat aaggaggata ttattttgaa aataaagtta gtatgcgata
    20521 gtttgtgcga tattccagat gaaatatcgg aaaaagatta tatagagata gttccactta
    20581 cagttatttt taatgatagg gaatatatag aaggagtaga tataaagaaa gaagagtttt
    20641 ataaaaaagt aaaagaaatc aagcaaatac ctaaaacttc acaagctaca tatatggagt
    20701 ttaaagaagt gtttgataag ttcataacag aaggttatca tataatctgt atgacagggg
    20761 ctgcaaacgc atcaggaact tttcaaagtg ctatgatagc taaaaatgat gtaaatgaaa
    20821 atgaaaaaat acatatattt gatactagaa atttatctct aggtagtgga caatatgtta
    20881 taaaggcatg tgaacttctt gaagaaggac tgggatttga agaaattata gatgaattag
    20941 aaaatactcg cagtagtgta agattgcttt ttgcaccata tacacttgat tttttaaagc
    21001 agagtggaag agttccagtt gcaactgctt tgataggaaa tatgttaaat ataaagccta
    21061 tatttttctt tgataatggg gaagctaagt tggtaaataa ggttagagga ataaagaata
    21121 tagcaagtaa attggtcgat ataattttag aaatgaatga agggtcttta gaaggaaaaa
    21181 tcgtcacaat aggttgtgga gataatttac acgattgtga aatattaaaa gatgaagtaa
    21241 ataaaagaat aaaagcaaga agagtattat ttactcgagg aggagtttct atttgctctc
    21301 atacaggacc agatatattg gctataagtt gttctaatta gtagtaatat aaggtttttg
    21361 catatatatg tgaagataat tttatattgt taatttatag tatttttgat aatatagtaa
    21421 atagagtgat atacgtgttt tttaataaga ggaggacaag cattaatgtt tgaacataaa
    21481 atatttaaaa tggattttgc aggaagagaa ctttctgtag aaataggaaa gatttgtgaa
    21541 atggctagtg gtagttgtat agttcgatac agtgatagta tggtaatggt aaatacaact
    21601 aaatcagcca aacctagaga cggaatagac ttttttcctt taagtgtgga ttatgaagaa
    21661 aaattatact ctgtaggtaa gatacctgga ggttttttaa agagagaagg caagccttca
    21721 gagaaagcta tacttacatc aagattgata gatagaccaa taagaccttt attcccaaaa
    21781 ggatttagaa atgatgttca ggtagttgca acagttctat cagtagacca agattgtaca
    21841 ccagatatag tagcaatgat aggttcttca atagcccttt ctatatcaga cataccattt
    21901 aatggaccaa caggttcagt atgtgttggc cttgtagatg gtgcatttgt tgtaaatcca
    21961 aacgcagagc aaagagaaaa aagttcaatg catctagttg tttcaggtac aaaggaagca
    22021 ataatgatgg ttgaagccgg agctgatgaa gttccagatg aggtaatgct tgatgctatt
    22081 ttatttgctc accaagaaat aaaaaaaata gttgagttta tagaaggtat agttgcagaa
    22141 gttggaaaag aaaaaatgcc tgttgaatta taccatgcag gagaagaaat aactcaattg
    22201 gttcgcgaat ttgcaactga taagatgaaa aaagcagttc agacttttga aaagttagag
    22261 agaatggaaa atatggatag agttaaagaa gaaactttag ctcactttga agaaacttta
    22321 gaagattttg aagattttgt tggagatata gaagaagtat tacaagatat aataaaagaa
    22381 gaagtaagaa aacttatagt acatgaaaat gtaagaccag ataatagaaa acttgaagaa
    22441 ataagaccta tatggtgtga aactggcatg atacctagag ctcatggttc agccatattt
    22501 actagaggac aaactcaagt acttaatgtt gctactttag gtgctttagg agatgttcaa
    22561 aaactagatg ggttagatga agaagaaaac aagagatata tgcatcatta taatttccct
    22621 gcatatagtg ttggagaagc tagaccatca agaggtccag gaagaagaga aataggacat
    22681 ggtgctttag cagaaagagc tttattacca gtaataccat ctcaagaaga atttccttat
    22741 gctattagat tagtttctga agtattaagt tcaaatggtt ctacatctca agcaagtgta
    22801 tgtggttcaa cattatcttt acttgatgca ggtgtaccta taaaagatat ggttgcaggt
    22861 atagcaatgg gacttataaa gcatgatggt aaggttgcag tgctttcaga tatacaaggt
    22921 atggaagacc atttaggcga tatggacttt aaagtagctg gaacagagta tggtataaca
    22981 gcaatacaaa tggatataaa aattgatggt atagataaag aaattctaca acgtgcttta
    23041 aaacaagcta aagagggtag aatccatata ctaggagaga tgagaaaaac tatatctcag
    23101 ccaaaaccag aactttcacc atatgcacct aagattgtta aaatgcaaat aaatcctgac
    23161 aagataaaag atgtaatagg acctggtgga aagataataa ctaagataat agacgaaact
    23221 ggagttaaga tagatattga acaaactggg gaagtattta tatcaggtat tgagattgat
    23281 atgattaaga aagctcaaga acttataaat aatatagtag ttgaaccaga agtaggaaaa
    23341 acttataaag gtaaggtaag tagaataatg aactttggag catttgttga gatacttcca
    23401 ggaaaagaag gattacttca tatatctcat atagctcatg aaagagttgc caaagttgaa
    23461 gatgtattga atatagggga tgaagttgaa gttaaagtta cagagattga tgaaaaagga
    23521 agagttaatt tatcaagaaa agttctttta ccaaaaccag aacataaaaa taaataactt
    23581 attatataat taaaagcaaa gtcttaggat tttgcttttt taaatacaaa aaagtagata
    23641 attttaaaat gatagttatg gatggcttat agttcttttt ctgtcagtct ttactcttat
    23701 aaagaagcta tttaaaagct gattaataga aagctttatt gatatatata gaatcactta
    23761 agttatattt agtgcataaa cagagcttat ttttaatagt attaaataag aaacaatttg
    23821 tacataatta gggggagttt taatgataat gatggtactt aataagagta agctcaaaaa
    23881 aatcataata ctaattttag tagtgttatt agtattatca ggaggggtaa tggtattaaa
    23941 gttatttaac aacaagccag tatttaattt atttaatggt gtagatttac catatgaaag
    24001 aggtacaaag gataaaagtg gatatgttgc attaacctgt aatatagacc ttggatggga
    24061 aacagagtac gtagaatcca ttttagaaac acttaaaaaa gaaaatgtaa agattacatt
    24121 taatgtaact ggaaaatggg cagaaaagaa taaggatgaa ttattaaaga taaaaaaaca
    24181 aggacatgaa ataggtaatc atggatataa acatttagat tattctacat tatcatatga
    24241 agacaattat gagcaaatag agacctctaa aaagataatt gaagaaatta taggtgaaaa
    24301 aactaaattt tttcaagcac cagcaggttc ctttggtcca gaaacagtaa aggctgctaa
    24361 agcattaggg tatacttcta tcaaatggga tgccgatact atagattgga aatacaaaga
    24421 ccaaccagag gtaatcattg atagaatgaa gaaaaaagac attaaagata gtagtataat
    24481 cttaatgcat ccaaccaacg caacgacaaa atgtatagat gatattatag ctattgtaag
    24541 agaaaaagga ctaaaagcag gaaaacttag tgatgtattt aagtagacaa agtataaata
    24601 tttggttaag attgtataag ttgtagttga attaattaaa agactgactt ataatgtaat
    24661 acatataaaa aataaatata attaaaacat agtgttttca ataatccaat atgaaacaaa
    24721 gaataaattg ttaggagaat gtaatgtata agacaaaaat actggaaaat gggcttacaa
    24781 taattggtga agaaatacca tatctaaagt caataacatt aggtatttgg ataaatgcag
    24841 gctctaggat agaagaagca caggtgagtg gaacttctca ttttatagag catatgatgt
    24901 ttaaaggaac caaaaacaga acatcaaaag agattgctag ttcaatagat aatttgggtg
    24961 gtcaaataaa tgcatttaca agtaaagaat gtacatgtta ttatgtaaaa ctaatagatg
    25021 agcatataga tacaggaatt gatgtattga gtgatatgat actaaattct aaatttgata
    25081 aaaatgatat agataaggaa agacttataa tattggaaga acttaagatg tatgaagact
    25141 ctccagatga cttatcttat gatttattag tggaaaatat atatgctaat gatggattag
    25201 gaatgaatat aattgggaca aaagaatctc tatacaatat aactagagaa tccatgttag
    25261 aatacttaaa taagtattat attccaaata acgctgttat ttctatagct ggaaacttta
    25321 actttgatga tatggtggag aaaatcaaat caaaatttgg acactgggaa aagaaaaatt
    25381 tgagtataga cataagtgag gctaagttta atccttgctt tatatcaaaa aataaagata
    25441 ctgaacaggt gaacttagct atgtgcctaa aaggtattcc ttttgaaaat gatgaagaag
    25501 tctattcaat ggcagtggta aataatattt tcggtggaag tataagctca aggctttttc
    25561 agaaaataag ggaagaaaaa ggtttagtat attcaatata ctcatcacag acattatata
    25621 ggaaatgtgg tgaattaggt atatttgcaa gcatgagtac agaaaattta caagatgtat
    25681 ataatttaat aaagaaagaa attgaaaata taagagaaaa ctatcttaca gaaaaagaaa
    25741 tatctgaaag caaggaacag ttaaaaggaa attatatatt ggatttagaa agtactagca
    25801 gtagaatgat gtctactggt aaatctatgt tacttagtaa aaaagtaaaa actacagatg
    25861 aaatacttga atgtataaat agtgttaata ttaattcaat aaagaaggtt gttgataagg
    25921 tattcaatat cgaaaatatt ggtacatgta tagttggaag agatgtagaa aagattctac
    25981 acttggatta atattgaaaa aagtaaatct ctggaggtat aaaatatgaa tttatctgaa
    26041 atagcaggta aggaaatagt taacttggtc acaggagaaa gattgggggt cattggagaa
    26101 tgtgacctta tactagatga aactacaggc ggtatattag cgcttctaat tccaaaagaa
    26161 aaaggatttt ttgccagaag aaaagataag tcatttttag aagtaccatg gagaaatgta
    26221 aaaaaaatag gtaatgacat gataattata gaatatgagg gatatattta ggaggaaaaa
    26281 atgagtattt tagtgcaaaa gtttggtggg acatcagtag aatcatatga aaaaatgaat
    26341 gaagtatgta agatagtaaa agcatacaaa aaaaatgatg aagaattaca attagtatta
    26401 gtagtttcag caatgggaag aaaaggagct ccatatgcta cagatacact tataaatttg
    26461 tgtagtgcag taaatgatga gccaagtaaa agagaacttg atttaataat gtcttgtgga
    26521 gaaataatat caggaacaat tcttgctaat ttattgaatg cacaggggat agattctgtt
    26581 ttctttactg gtcagcaagc aggtataatt acaagtgatg aatattctaa tgcaaagata
    26641 aagtatataa atcctaaaaa ggtaaaaaga gctttagatg atggtaaggt tgtagttata
    26701 gctgggttcc agggtgtaac agatgatgga gaaataacta ctcttggaag aggcggaagc
    26761 gatacatcag cagttgctat tggtaaagct ttagaatgtg aaacagttga aatttacact
    26821 gatgtggatg gtataatgac tgcagaccct agagtagaac caaacgctaa agtattgagt
    26881 tttatagact atgaggaagt attccaaatg gctgacaaag gtgcaaaagt aatacatcca
    26941 agagcagtac agttggctaa aagtggaaat ataactttag ccataaagaa tacaatgaat
    27001 ccaacttttg aaggtacaaa aataggttct ttatgtagac acttagaaga caatatagaa
    27061 tatgaacaag aaagagattt taaggttgct gttgcaaata aagatagcgt agcacaagtt
    27121 aagattaagt cagcagaaga agtgtttaca gaagtattaa atgagataga aaagaaactt
    27181 ataactatag atatgataaa tttctttata tcagaaaaag catttgttgt agaagatgct
    27241 gatataaaat cattaaagga aatactagat aaatttgaat tagattatga agtaaataga
    27301 gactgtgcta aggtaacatt gatatgttca agaattgctg atgaaatgtc tggtatcatg
    27361 tcaaaggtag ttagaggatt atcaaaagca ggggtatccc ttttacaaac atcagactca
    27421 aatatgacta tatcttgttt agttagtgaa gaagatatgc atacagcagt acatgctata
    27481 catcaacaat tttatttaaa ataaactgta taatacaatt attttttaat caaatttaag
    27541 aatttcatat atatcttaat atatttgatt tatttggtaa aactaaatta aatataattt
    27601 tgataggagt ttttttatga aagattatag agaagataat gaaagatttg ataaagataa
    27661 ttgtagtgaa gaatctagtt gcgaagagag agaatgtata aagcaatttg gtaccaatga
    27721 aatgccacct caacctccta aagatattca atgtataact ataattggag aaattgaggg
    27781 acattttata ggaaatcctc aaaaaaaagc tacaaagtac gagcatataa ttccaatgct
    27841 atactcaatt gaggaaagta atgatgttaa aggagtgtta gttgttttaa acactgttgg
    27901 aggagatatt gaagcaggtt tagctattgc agaactttta aacagtacat ctaaaaaagt
    27961 agttactctt gttctaggag gaagtcatag tataggtgtt cctcttgcta cagcaggaga
    28021 ttactcattt atagcaccaa cagcaaccat gattatacat ccagtgcgaa ctactggtct
    28081 tgtgattggg ataaatgaga cttttgagta ttttaaaaag atgcaagata gaataattca
    28141 atttataata agaacatcaa atatcaaaaa agatgtactt gaaaaactta tgcatgaaaa
    28201 agatgaactt gtaagtgatg ttggtagtgt attaatagga aaagaagctg tggattatgg
    28261 tcttatagat gaagttggag gactaaaaga agcacttaaa aaattaagag agcttataaa
    28321 agaatctgag gaagaaaaaa ataatgatta aaatttgttt aaataataaa atttatgata
    28381 taatgatgta ataaagtagg agtgttagtg ttaaggcatt agcacttttt gctgtgaagg
    28441 ggtaaaactt gctaaaaata aggagtgata ttaatggcca cgaaaaagaa gaaaaaaaag
    28501 aaggtaaata ccttaagttt taatgcagaa tatcataatt taataacaat atttataggt
    28561 gtatttttat tatatagttt aaattctaat tctatgggat ggatacctgt gttaatgcaa
    28621 aatcttttta agggactatt tggagggctt tcaatagcaa taccatttat agtaattata
    28681 acaggacttt taggattttt tgatggaaat gaatatatat ataggctaag aaagactaaa
    28741 ctatactaca taattatttt atttatattt gtattttatg gattattaaa tgcaggaact
    28801 cttcctgtag atagcccact aaaaggtaac atgtttgatg atgttatgaa attgggtgta
    28861 agtggacaag gtagtggttt gatagcaaca actattgcgt attatatgag taaaatattt
    28921 ggtatagcag gtggatggtt aataagtatc ttcgcactta tactttctgt aatgtttata
    28981 tttaatattt ctataaaaga tttggtgtca aatgcaaaat caaaatctaa agcatctaaa
    29041 gacagcaatt taacgtttaa agataaaatt gcaaatatga aaaaatctgc aatagatatg
    29101 atgactgatg aagtagatga tactactata aataaaaaac caggtttctt taaagggtta
    29161 atgtcaaaag gtcttaataa tgatgatgaa gaagatgaat attttgaaga agaaaatagt
    29221 gatggtgtag atgataaaac tataaaaata gttggattta ataaggcaga agatgagtat
    29281 ttagaaatat tagaaggaac tcaaagtatg ccagagttag atgttttaaa agaattacaa
    29341 aaagctacca atgaaaatcc agttgtagat actaaaccag aaaaaaaagt tgatatagct
    29401 aaacctaatt taaatataga aaaaactcaa cctatgagta ttgtagcaga gccagttaat
    29461 gaggattatt caaactataa aaagcctagc attgagcttt taaataaagt aaataagaag
    29521 tctgatgaaa atggtaaaaa gaaagtgttg aaaaatgctt ctcttttaga aaagacacta
    29581 tcagattttg gagtggaagc aaaaattaat caagttacag ttggacctac tataacaaga
    29641 tatgaaatac aaccaagtcc aggagtcaag gtaagtaaaa ttgtaaattt aactgatgat
    29701 atagctttaa gtttagcagc taaaagtata agaattgaag ccccaattcc tggtaaaagt
    29761 gcaattggaa tagaagttcc aaatgaagaa gctcaaatgg ttggtgtgag agaagttcta
    29821 gaaagtgaag aatttaataa ttttaactct cctcttgcaa tgggacttgg aaaagacgta
    29881 gcaggaaaaa ttataattgg agatataggt aagatgccac acttactaat agctggttct
    29941 acaggttcag gaaagagtgt gtgtgtaaat acattaataa gtagtatttt atataaggca
    30001 aatcctgatg aagtaaaatt actattaata gacccaaaag tagttgaact tgcaaattat
    30061 aatggaatac cacatctatt gataccagta gttactgacc ctaaaaaagc tgcaaatgct
    30121 ttaaattggg cagttacaga gatgaataga aggtacaaat tatttgcaga tgctcaagta
    30181 aaagatgtaa caagttataa tgagaaagcg gaagaaaaac ttcctaaaat agtaataata
    30241 atagatgagt tagcagattt aatgatggca agtgcaaatg atgtagaaga ttatatttgt
    30301 agattagctc aaatggcaag agctgctgga atgcacttga ttgtagcaac tcaaagacca
    30361 tctgttgacg ttataacagg ggtaataaag gctaatatac catctagaat agcatttgca
    30421 gtatcatctc aaactgattc aagaacaatc cttgatatgg gtggtgcaga gaagcttcta
    30481 ggtaaaggtg atatgttatt ctatccactt ggtgcagcaa aaccagttag acttcaaggg
    30541 gcattcatat cagagagtga gtcggaaaaa gtaatcgatt ttgtaaaaag tcaagttaaa
    30601 gatggaataa aatatgaaga agatataatt gaaactatat ctaaggttaa tacttcaaag
    30661 ggtagtgatg aggatgagtt tttgagtgaa gcgatagaat ttgtcgtgga aagtggtcaa
    30721 gcatctgcct ctatgctaca gagaagattt aaaataggat tcaatagagc agctagactt
    30781 atagattcta tggaagaaag aggaataatt ggagcaagtg agggaagtaa accgagaaag
    30841 gtattaataa gcaaacagga tttgcagaat ttagaaggtg aatagaattg tcagttataa
    30901 aaatagaaaa agcacttaag ctagataaag taatatttgt agatgtgaga actgaaggag
    30961 aatatgaaga agaccatata ttgaatgcaa tcaatatgcc tctttttaaa aataatgaac
    31021 ataatgacgt tggaacaatt tataagatgc agggaaaaca tgaagcaata caaaaagggt
    31081 ttgactatgt atcgtataaa ttaaaggata tatatttaca agcagcagaa cttgcattaa
    31141 attatgataa tattgtcata tattgtgcaa gaggcggaat gagaagtggc agtatagtaa
    31201 atttattatc atcattaggt gtaaatgtat atcaacttga aggtggatat aaggcttata
    31261 gaaattttgt attagagtat ctaaggcatg taatggatac aaaaaacttt gtatctgttc
    31321 atggattgac tggtacagga aagactgatt tgttacattt attagatgaa aaaggaatag
    31381 atgtactgga tttagaaggc atagctaaaa acagtggttc tacctttggt tttataactt
    31441 ttgataaaaa gccaccttct caaaagaact ttgaaacaaa actatttgaa agtatatttt
    31501 tttcgaaaga gaattatata tttgtcgaga gtgaaagcaa acgagttgga agtgttacag
    31561 ttccaaatga aattttcgag gcaatgacac gagaaggaca tcatatatta ttagaatgtg
    31621 atattgaaaa tagagttgat agactttgta gagattatat ttatgataaa gatgaaggta
    31681 atatattgat tttaaaagaa tgtataaaca agtttagaaa aagattgggt aataaagtag
    31741 tagatgatta tattgattta ttagaatcag gtgaatataa agaacttgta aaaagatatc
    31801 ttttagaata ctacgaccca ctttatatgc attcagtaga aaaatataag tatagtaaga
    31861 ttataaattt taatgataca caaaaagctt tagaagatgt tatagactta tatgagagta
    31921 tattggaagg agaaagtgaa tgttaaaaat agcattagaa tcattaggat gctcaaaaaa
    31981 tttagtagat gcagaaataa tgatgggaat tttaaataac aaaggctata aattaattgg
    32041 agattttgaa gaagctgatg taattatagt aaatacatgt ggatttatag agtcagctaa
    32101 acaagaatct atagatacga taataaactt tgcagagtta aaaaaaactg gcaatttaaa
    32161 gttgttgata gtaacagggt gtctagccca aagatattct gaagaattaa aaactgaaat
    32221 tcctgaaata gatgctatag taggaacagg aagctatcaa aatatagata aaatacttaa
    32281 agaattaagt gaaatgcata aaattgtaag ccttaatgat atagagtttg tatttaatga
    32341 agatttacca agatatatat ctacacctag ctatatggcc tatttaaaaa ttggagaagg
    32401 atgttccaat aattgtactt attgtataat accaaaacta agaggtaaat atagaagtag
    32461 aaaatttgaa gatataatta aagaagctaa gaaattagct gaatctggag taaaagaact
    32521 tgtggttata gcacaggata cgacaaagta cggttttgat ttatatggaa aagaaagatt
    32581 atcagaactt ttagaagaac ttgcaaaaat agatggattt aagtggataa gggttatgta
    32641 ttcatatcca gaatctataa cagaagaatt gatacaagtt ataaagaaat atgataatat
    32701 atgtagttac tttgatatgc caatacagca tgcaagtaac aatattttaa aattaatgaa
    32761 tagaaaaact actaaagaag atatattaaa taaaatcaat ctgataagaa gtaatatacc
    32821 agatgctata cttagaacta caataatagt agggtttcct ggtgaaacag aagatgactt
    32881 caaacaactt gtggactttg ttgaagaggt taaatttgat agacttggtg catttgcata
    32941 ttcaagagaa gaagatacac cagcagatag attgccaaac catatagatg aagaagtaaa
    33001 aatacaaaga agagatactt taatgatgat tcaacaaaaa atatctgaag aacttaatga
    33061 caaaaagata ggaaaaactt atgaggtgtt aatagaagaa cagatagaag ataatgtata
    33121 tacaggaaga acacaaggtg atgcagagga aattgacagt atagtgtatg taaaaagtgt
    33181 agacaatctt gaagtaggag aatttgtaag tgttcaaata aatgatgcaa tggaatacga
    33241 tttaatggga gatgttttat atgaacttgc ctaacaagtt aactttgttt agaatatttt
    33301 taataccagt atttgtttta attatgttgc tcaatatacc aaataaattt ttaatagctt
    33361 gtattatttt tataatagct tcaattactg atgctttaga tggaaaaata gctagaaaat
    33421 acaatctagt cactgacttt ggaaaattta tggacccatt agcagataag ttattggtta
    33481 tttcagcact tacatgtatg atagaagacc atcttgtaag tagttggatg gtaattataa
    33541 tagtggctag agagcttaca gttagcatat taagagctat agctgctgct gatggaaaag
    33601 taattgctgc tggaaacagt ggaaaactta aaacgataac tcaaatggta tctatagtgt
    33661 ttttattgtt aggtgctcag tttgaaaatg tattgatatt aaatattggt gaaattctca
    33721 ttcttatagc tacactactt acattatatt ctggatggga atatctatat aaaaataaag
    33781 agctttttat gtcttctaag taaaaaatta tgctttaata aataaaaact aatgattaat
    33841 aaataaaaaa taacaactta aaattttaaa aatgtaaaaa agggtatact attatagtat
    33901 acctcttttc attttaagaa tattttcttt tgtgtatata attattaata atggtttata
    33961 aataaaatgt aatgaataaa aatataatca ttagtagcgt ttttaaattg actaggtttg
    34021 atttatgtta tataattaat atataacaag agaacaaatg tttgtagata aatgaaggga
    34081 tggtatagag atgagtgtag atcaagaaaa attaaaagcg ttgaatgaag ctttaggtaa
    34141 aattgaaaaa gattttggta aaggttcagt aatgaaattg ggagaagcaa catctatgtc
    34201 tatagatgtt atatcaacag gagcgattgg tttagacata gctattggta taggaggtct
    34261 acctagaggg agaatagttg aagtatatgg tccagaatct tctggtaaga ctactgttgc
    34321 gcttagttgt gtagcatcag ctcaaaaaga tggaggaata gctgcattta tagatgcaga
    34381 acatgcactt gacccagtat atgcaaaagc tttgggtgtg gatgttgata acctaataat
    34441 atctcaacca gatacaggtg aacaggcttt agagatagca gaggcattga taagaagtgg
    34501 agcgatagat ataatagtaa tagactcagt agcagcatta gttccaaagg ctgaaataga
    34561 tggagatatg ggtgattctc atgtaggttt acaagctaga cttatgtcac aagcacttag
    34621 aaagttaact ggttcaatta aaaaatcaaa ttgtgttgct atatttataa accagttaag
    34681 agagaaagta ggaataatgt ttggaaaccc agaaactact actggaggac gtgcactaaa
    34741 attctattca tcagttagat tggatgttag aaaaatagat acaataaaac aaggtgataa
    34801 agttataggt agtagaacta gagttaaagt tgttaaaaac aaagtagcac caccatttaa
    34861 gcaagctgaa tttgatataa tgtatggaga agggatttca aaaattggag accttttaga
    34921 tatagctgct gatgtagata tagttaaaaa atcaggttca tggtatagtt acaatgatac
    34981 taaacttgga caaggaagag aaaatgttaa aaaattcttg gaagataatt tagatttaac
    35041 tactgaaata gatgagaaag ttagagcatt ttacaattta aatgaagaac atgaagaatc
    35101 aggtacttca gtatcaaaag aaattgtaga agaataagta aaaatacaaa aaaataacta
    35161 gtaattaaag acatgccacg ctccctatta gcttgacaca atgttgaaaa aattataaaa
    35221 ttaagtgagg ataaaatatc taaatttaat tttaattatt aggaatattt tttaatcata
    35281 gtgatgacat tttgggatta ttaacgaagg aggtggatgt catagatagt gtagtaatga
    35341 ttgtaatagg tgcagctgtt ggtattatag ctggatattt tgttagaaaa aatatatctg
    35401 aagcaaaaat tggacaagct gaaaacttgg ccaaagaaat aatagataaa gcacaccacg
    35461 actcagaaac agtacaaaaa gaaaaattac tagaagctaa agaagagatc cataagtgga
    35521 gaacagaagc tgaaagagaa aacagagaaa gaagaacaga agtacaaaag tatgaaaaaa
    35581 gagtagtaca aaaagaagaa gtcttagata gaaaattaca aaatttagaa tctaaagaag
    35641 taaacctaag tgaaaaatta aaggtagtgg aaaagaaaga agaagaagtt gaagtaataa
    35701 aaactcaaca gttagaaaaa ttagaaagta tatcaggaat tacttctgat aaggccaaag
    35761 aaatcatctt aaccaatgcg gagagagatg taagacgtga aatgtctata atgataaaag
    35821 agatagaaag tcaagctaaa gaagaagctg ataagaaatc aagagaaatt ataggatatg
    35881 caattcaaaa atgtgcagca gatcacgtag ccgaaactac tgtaacagta gtaagcttgc
    35941 ctaatgatga aatgaaaggt agaataatag gtagggaagg tagaaatata aggactttag
    36001 aaaccttaac aggtatagac cttataatag atgacactcc agaagccgta attttatcag
    36061 gatttgatcc aataagaaga gaaattgcaa gaattgctct agaaaaatta atagcagatg
    36121 gaagaataca tccagctaga attgaagaaa tggttgaaaa ggcgagaaaa gaagttgaca
    36181 atattataaa agaatatggt gaacaagctg catttgaaac tagtgtacat gggattcatc
    36241 cggaattaat aagattatta ggtagactta actatagaac tagttatgga caaaatgtac
    36301 ttaaacattc tatagaagtt gctcatatag ctggtataat ggctgctgaa ataggagcag
    36361 atattagatt ggctaagagg gcaggtttat tacatgatat aggtaaggct gtagaccatg
    36421 aaatggaagg tacacatgtt gaaataggta tggatttact tagacgttat aaagagtcta
    36481 aagaagttat acatgccatg agtactcatc atggagatta cgagcctcaa acagtagagg
    36541 ctgtattagt tacagcagca gatgcaatat cagctgctag acctggtgcg agaagagaaa
    36601 ctttagaagc atatataaag agacttgaaa aattagaaga aatagctaat tcatatgaag
    36661 gtgtagaaaa atcatttgct atacaagctg gtagagaaat aagaataatg gtaaaaccag
    36721 aagctataaa tgatgaagaa atacatctat tggctagaga tatgactaag aaaatagagg
    36781 atgaacttga atatcctgga caaataaaag taagcataat aagagaaaca agagctatag
    36841 agtatgcgaa gtaaaagaaa taatatctaa ataataagaa aatattttta gaaatttaaa
    36901 tttgccacaa gatatgataa tatatcttgt ggcttttaaa ttgtttaagt ttaattttat
    36961 ataatgcaaa aataatctaa gtgctaaaag tgctacttac aaaagattaa taaaataaag
    37021 ctttatatat gtattattta tataattatc caagtttgtt tgatataatg aagttgtaaa
    37081 ataaatatat tactacttgg aatgcttgta cttaaataag atgaaaatac aatgacgcta
    37141 aagtgaggat tagagagagg gtacactatg aaaaaaaata cacttttaca tacaaatatt
    37201 atggtttgca ttattattac tcttggtttt gtggctacct ctataattgg atatcaatct
    37261 aataccaata tttttaaaaa tgatattgaa catgtatcta cattggctgc tgaaggtatt
    37321 tattatcaga tagataaact actttcagaa cctataaatg tatctcttac aatggcaaat
    37381 gacagtctat taaaaaattt tttagatggt gagaaagaac atttaaatga tgaagaattt
    37441 atttataaat tacaagatta tctgaatgtg tatagaaata aatattcata tgattctgtt
    37501 tttttagtat caactaaaac caataattat tatcatttta atggacttga tagaaatctt
    37561 agcgctaata actcagagaa tcaatggtat tatactttct taaagaatga tgatgaatac
    37621 tcattaaatg ttgataatga tgaagcaagt aacaatagta ttacagtctt tgtaaattgt
    37681 aagataaaag atgataatgg agcaactatg ggaattatag gtgttgggtt aaaggttaat
    37741 tcattacaaa tgttgcttaa aggatataat gacaaatttg atgttgttgc acgcttaatt
    37801 gatgataaag ggtttgtaca attagcagta gataaaaccg gtcatgagaa tataaatttt
    37861 tttgaaaatt ctagtagcga cttgtctaat tcgaaacaat taatattaaa caacaaaaag
    37921 gaacaaaaga gtttttggta ttcatctgaa aaatcaaaaa gttatattgt atgtcagtac
    37981 atacctagtt taaaatggca tcttgttcta gaaaatgatt ttactttaat gattaaacag
    38041 cttcatctgc aattttttaa aggcattgca gttatagttt taattataat atttgtactt
    38101 tttactatta cacatgtgct taaaaaatac aaaaaccaaa taataaagct tagtgtttca
    38161 cagaaattgg aatatcaaaa attgttgagc aaatctactg aaggtcttta tgaaaatatt
    38221 tttgaaattg atattactaa caataaagct ggttgtgaag atgagaatag gtattttcat
    38281 gaatttggat tagggcttaa taccccatat gatgaagcct tacatacaat agctaaaaac
    38341 caaatcaaag aagaatatgc acaaggttat ttagatacat ttttacctga aaatgtaatt
    38401 aaacgttata atagcggaat taataatatc agctatgatt ttttaattaa aaaagatgaa
    38461 gaaagttatc attggattag aattaatgca cgaattttct tttggacatc agatgaatct
    38521 gttcgtatga tatcttatcg taaaaatata gatgaagaaa aaaatagaga attactactt
    38581 ctcgaacgtt cacaacgtga ttctcttaca gggctttaca ataaagctac aacagaggaa
    38641 tttattcgtt catttttaaa aaatgaaagt aatcaagatt tagaacacgt ttttctaatt
    38701 ttcgatattg ataattttaa aaatgtaaat gataaatatg gtcatgtatt tggagacttt
    38761 gttataagtg aatttgcctt agaattaaaa tctcaatttc gtgaggacga tattgtggga
    38821 cgtattggtg gagatgagtt tgtagtatta ataaaagact ttaataataa taactatgtg
    38881 cttatagaca aattagagcg tttttgttct aagattaatc agaaaaaatt tgttgagcaa
    38941 gcagaatttt ctattgcatg tagtattggt gtagcaatgt atcctgaaca tggatatgta
    39001 tactctgaac tatatgagaa agcagaccaa gcactttact atgcaaaaag ccatgggaaa
    39061 aattcttatc atatattttc agaggattct attgatagta tttcatttca tatagactct
    39121 agagatataa aagtattatt agaaatggct actgatggtg ttgtcaaatt tgcttgtatg
    39181 gaaaacttaa aattacttta tttcaaccaa aaaatgcttg aatttactgg atattcagct
    39241 caaactgtta cttctatagg ttttgatatt ttttcgcctg tattacaaga cgacttacaa
    39301 atggtactag atacattaag agaagcaatt tctaaaagag agacttttga aatagttttt
    39361 cgtttttgtc atacagatgg tcatattttt cctgtaagga tgcaaggtaa gtttgtaagt
    39421 gaattatatc aaaatcgtta tcctgttttt tatgcgattt atacagacat atcaagtatc
    39481 aatactttac tagaaaataa atagtaaata ctacagtaaa atctaaaaat aggatataat
    39541 agtattaata taagaagatt ataaataaaa cctgctaatg aaaatagatt atttttagca
    39601 ggtttttaaa ttttttataa taaatcttat ttttggctaa caagatttat tttttttaag
    39661 actttactta ttattttaag cattaattat gaaatttatc attttattat ttacagaaag
    39721 tattttataa caataaatat ctatacttat tgcaaaattt ttttagcagt tttgtattta
    39781 tttgtattta ttttgaattt atttactaaa tttgcctata aaaagtatat ataaaaatat
    39841 agttcaaaat ggtataatta agtataacag aaggaggtaa tgtaagtgag ccaatacata
    39901 aatataaatt ctcagttaga cgccctaaaa ttgaaggagt tcgagaaaat attttacgaa
    39961 aatatcaatg aggacgttaa agtagtgcca aaagttactc cttttaaagg aataaatacc
    40021 gatttgttgt atgttaaaga tggaagaatt ttatttataa aatttatgga tacaacagaa
    40081 gatatatttt ttattttaga agaagagtta cttgaagtta tgaatgagga atatgaactt
    40141 ttgaaactaa aaatgggaca aaagaataga aatataagtt ataactacgt ttacattatg
    40201 ccatacgttg aagttgagga aacatatgaa tttgaagaat ttgttaataa taatataata
    40261 gataaaaata agcttcaaga tataatgaat aagggttcgc tggaggaata tcttaatgat
    40321 gagaatgatg aaataaactt aaatttattt ttactagaca tatgttcaga atactatata
    40381 ataaatgata aacttcatct aaatgaaaaa tttaaaaaaa tttcattcta caatgatgac
    40441 tataagtata cagcaactat gatggaagac gttcaaatta aagatgttgt ttctattaaa
    40501 tatgggaata cacttattga aggtggttct ggtataggca aaacagcaat tatgctttct
    40561 agggctataa aattagcaaa agtatatcca catcataaat tagtcatatt tacacataca
    40621 aaacaactgt gcaatgagct tcgagagagg atagagttat tatacaaaga taataacaat
    40681 ttagaagttc acacatttag ttcttttata tttaaactag ctaaaaaatt caatttaatt
    40741 gtagattata acatgttaaa aaatgattac gaaaagacct ttaataatct tgtaaaacaa
    40801 gcacagaaca ttataaaaaa taaaaatatg ttcaaggcta tttttattga tgaagcagaa
    40861 agctttttag agtatgaaat agattttata agggagtttt tatataaaac aaaattcata
    40921 tttaatgtgt gttcttgtaa ctccttgaat atctctaata gattgaatat atttaaaaaa
    40981 ttatataatg gtatagaatt tgatgataag attatcttaa gtaaaaatta tagacaagct
    41041 aaagagattg tagattttac aaataaattt tcaaataatt ctaactcata tatcaacgaa
    41101 ttaagaccaa atacagaatt tagtactttc ttttatacta aagcacttag aggtggaaat
    41161 aagtctgttg atataataaa agtaagtgac cttgatgacc aaataagttc tgtaatctgg
    41221 gaaattgagt atttaataag taaaaaaggt cttgattatt ctgaagttgc tatcgtttat
    41281 ccttacaata agaagaaatt aaaaagtgga aaaaccattt actttcaata tatgttaaaa
    41341 aaagcattgg aagaggcaaa aattccttat atatgtgcag aagataattt gacaaatata
    41401 agtaagaagg taggaactac aatagcaaat atatatgcaa taaagaattt agagtataaa
    41461 gcggtaatag tatgtgaatt agagatgtta tacaatcaaa ctataaatga tattgaacaa
    41521 gattatcaag taaatgactt tgtaggagat ttaaataagg tttatttagc tatgagtaga
    41581 gcaacagact acctaagtat agtaacaact tttaatgaag aagcaagtga tataataaga
    41641 cttattacag aatcaaaaga tatttagtaa aaaaatattt gtaatttaag aaaataaaaa
    41701 gagactgaga tttgtaaaat aaactttacc aattcagtct cttatttttt agtctacttt
    41761 cccaatatta tcaagaggta atagtctcac cataacctta ccaagaactt catcttcatc
    41821 aaccatacct acatcaggga atctactatc attactattt tctctattat ctcccattgc
    41881 aaatagttta ccttttggaa caacagtatc tatatctcca gaagtatagt tattgtgtat
    41941 atatggttca tttaataatt ttccatttac atacacttta gaatttgata tttttattct
    42001 gtcaccttca gtagctataa ctctttttat taagtctttt ttctttcctg tttctccatc
    42061 aactaaattg gttttaaaaa ctacaatatc gcctttttca ggtttaccaa ccttgtatga
    42121 tatcctattt aaaatcagat aatctttatc atctaaagta ggatacattg attctccact
    42181 tacaatagaa ggtcttataa attgtagaat tacaaatgca agtacaatag ccatagctat
    42241 tgacttaatc caatcaaata tttctttttt aacactcata acttttctcc ttatcatttg
    42301 ttattaaatt agaatacatt ataatagaaa gaaataagca agaaccacta tacccaagac
    42361 tattctataa taaccaaata ctttgaagtc atgttttttg atatagtcca taaaaaattt
    42421 aataactaca attgaaacaa caaatgctac tacagaacca acacctaaaa taagccattc
    42481 aaatccagta aatccaaatc cagcttttag aagttttaat gcactagctc caagcattgt
    42541 agggatggct aagaagaatg aaaactctgc tgctacatat ctagaagtac caagtaatac
    42601 tgcacctatt atagtagaac cagacctaga tgtacctggt ataagagcta gacactggaa
    42661 taatcctata caaagagcta atttatatgt aacttgagaa aaatctttat acttaggttt
    42721 tttatttctc ttttcaagcc ctatcattat tataccatat acaataagag caatagcaac
    42781 tactgttgaa ttaaagaata atctatctat atcatcttca aacagtattc caataactcc
    42841 agatggtata actgcaatta taaccttaag ccataaacgg actgtttctt ttttctgctt
    42901 aatattcttt gcgctatcaa aaggatttaa ttttctaaaa aatataacaa gtacagctaa
    42961 tattgaacca aattgaatta caactaaaaa tgtacttata aatgtatttg aaaaattcaa
    43021 ttttataaac tcatcaacta atatcatatg ccctgtacta cttactggta gccattctgt
    43081 tattccctga actatgccca aaatgactgc cttaaaaatt tcaagcaaac taaatgtttc
    43141 caaatcaaaa tacctccaat acaaatttat agtagcatat tttattcata gattaataaa
    43201 aaaatgccac cttttactaa cttataatac catagtactg tgtagtgcac aatggaagat
    43261 tgatattttc ataaaacctt cataatataa caaaataaac ttaaaagtta tctattgaat
    43321 aaggggtata tttgaggggg aaatatatgg tatagtaaaa gtatagctat tttattttat
    43381 gaaaaggaaa ggagttatta tgtatagtac aaaaatacta gatgattatg agtatgaaac
    43441 ttttaatgtt ttttctacaa aattaaatcc aaacacgaaa cacgattatt tatcaaaggt
    43501 tatactgttt aaagaatttt taaaagggaa agaattgatt tatgctacga aagaagattg
    43561 taaaaatttt gtagactata tacagactaa atatgcgaaa tcaacttgtg aaaagatata
    43621 tagttattta catagttttt ataacttctt aaaaaaagaa ggatacatag atataaatcc
    43681 atttagatat gtagaaaaac ccacagtaac aagaataaaa acaaaagatg atgttctaag
    43741 tatacaagaa ataaataaac ttataggtat cctaccaaaa ttaaatataa gagatagagt
    43801 aataatagtc tgtctagtta ctactgggtg tttattaaat gaattagtta gcttaaagtg
    43861 gaaagacctt atggtagatg aaaacgataa ctcatatgta aggttaggta agggaagaaa
    43921 agaaagagta gttaaattac atccatactt ttttaaactt ttagaagatt acaggaatta
    43981 ttctgggctt ccagaggtaa taatacctag tgatgatttt atttttacaa ctcaaaagag
    44041 taattctata acagatagaa atgtcagact tatagtcaaa aaagcattag atttagctgg
    44101 actttctcaa tattcagcaa gagattttag acattcattt gcagcaataa gtttaaggct
    44161 tggggcagat gaaagtgatg taaaaaatca gcttggatgg agtgataaat actatgcaat
    44221 tagatataag tatgtattaa attttgttga tagtgaaata gtggattact taattgaaaa
    44281 agataatttg gatataaata aaaaatagta ataaataaaa aatactatat ataaatgtga
    44341 acaaaaaata aaaaagtctt gttatagttc gtaaaattat atataattat ttatgtacta
    44401 atgtgtacac agtaatcaca cgacaaaaga tttaaggaag gtgctaatat aatgatagat
    44461 aataaatata ctacttactc aaacccactt acagacagat attgtagcaa ggacatgagt
    44521 tatatatttt ctccacaatt taaattctct acatggagaa ggttatgggt tgcacttgca
    44581 gaaggtgaac atgagttagg aatcaatata actgaagaac aattagatga gctaagagct
    44641 aatatagata atataaattt tgatgaagct agaaaaatag aaaaagaagt aagacatgat
    44701 gtaatgtctc acgtaaaagc ttatggttta caatgtgaaa aagccaaagg aataatacat
    44761 ttaggagcaa catctgcata tgttggagat aatacagatg ttatacaaat gaatgaagct
    44821 ttaaaacttc taagagtaaa gcttgtaaat ttaataaaca atttaaaaga atttgcactt
    44881 aagtacaaag atatgccagc tcttggattt acacacttcc aagctgctca acttacaaca
    44941 gtaggaaaaa gagcttgttt atgggctcaa gatttaattc ttgatttgga agatttaaac
    45001 tatagaatag acaacatgaa gttaagagga gttaaaggta ctactggtac tcaagcaagt
    45061 tttttaagct tatttgaagg agaccacgaa aaagtaaaag agttagataa gaaaatttgt
    45121 aaaaagatgg gataccattc ttcctatgca gtaagtggac agacatatac tagaaagtta
    45181 gattatcaag taataagcat actatcaggg atagctcaaa gtatgcataa gatgactaat
    45241 gatataagac ttttacaaag tttaaaagaa ttagaagagc catttgaaaa aaatcaaata
    45301 ggttcatcag caatggctta taagaggaat ccaatgagaa gtgagagaat agcatcttta
    45361 tctaagtata taatctctga atctataagc cctgcaatgg tacaagccac tcaatggtta
    45421 gagagaacat tagatgactc agccaataag agattggcaa tacctcaagc atttatggca
    45481 gcagatgcta tacttgaaat agggataaat gtaactgatg gtttagtagt atatgaaaat
    45541 atgataaata gacgtgtaaa tgaagaactt ccttttatgg ctacagaaac aatacttatg
    45601 gaagctgtaa aaagaggtgg agatagacag gaattgcatg aaataataag agaatattct
    45661 atgaaagctg cctatagagt aaagcatgaa ggcaaggata ataacttaat tgaacttata
    45721 atgaatgatg attcatttaa aatgagcaag gaagaaatac tatctataat ggacccaaaa
    45781 aactttatag gtagagctcc agaacaagtt gtagaatttg taaatgaagt tgtagaacct
    45841 gctataaaag attataagga agacttagga aaagtagatg tagacctaag agtatagaat
    45901 tgtacagata aaatataatt aaaaacggct atcttattta taaataagat agccatttta
    45961 atgggctagc gtcttctctc caccgctgtg aagagttctt ctttacttac tataccatga
    46021 atttaataaa atagctatat aaataatatt taataggaat aatataaata aaaataaaaa
    46081 aatgataaga ttactaatta aatttaaaca ttcagcaaaa actattgtac ttgcaatatt
    46141 tttaatgtaa actgaaataa agttatttag ataatttaat aaattttaaa acaaccgagg
    46201 tgattatatg aactatatag taaaagatac cataagtttt ttagtaaaaa catttcctaa
    46261 gatttattct agcctgtact tagaagacct aaaaaaattt gcaccagatt ataatgtaaa
    46321 taaaacacaa ttaagagcat tagttttcat aaaaaattat ggagtaataa gtatgactga
    46381 tttatgctct aagttaaata tagaaaaagg cagtttaact agtatggttg atgatttaac
    46441 tgataagaag tatgtaacaa ggaaaagaga tttagttgat agaagaaaat atctaattga
    46501 cattactgaa gagggagata agatagcaac tgattttatg gataaattaa gtgatggttt
    46561 aggagaaaaa ctttctaaat taactgaaga agacagaaaa aaatatttag aagcaataaa
    46621 tactcttcaa tatatattaa acaaagaaga atttagatag aaaaaatata aaaatttttg
    46681 tatttaatgg tactaagatt aattctttgt tattcattta actatataat tataagtcta
    46741 aatacaatct aaaaagtaca tcttaaattt tcttgatgta cttttttata atttctctaa
    46801 ctaaggcaag acaagtttta gatgctttaa ttaaagtgaa taaagattac tggccaacac
    46861 tatataaaga aaatgaaaaa tagtcaggtc taagtcttaa ttaatatatt aattaagaaa
    46921 tgtccccttt tcaaataata tctgaaaagg ggacatttta tatcattaaa tttatataaa
    46981 ttaagtttaa aaaattattt ttgtaaagct acatattgct ctaaaccttc ttttaataat
    47041 gtagcagctc tatgaaggtc tttttcattt aagatgtaag ctaatcttat ttcatcacgt
    47101 cctaaacctg gagtagcata gaatccttca gcagggcaaa ccataactgt ttcattatct
    47161 ttattaaatt cttttaacat ccaaattgtg aaatcttctg cattttcaac tggtaattta
    47221 gcaactatat agaaagcacc agttggtttt ttacatataa caccttcaac cttcattaat
    47281 tcattgtata atacatctct tctttttttg tactcttcat taacttcttt gaagtaagaa
    47341 acaggagttt tgtataactc aacagctcct atttgctcta atgtaggaac acaaagtctt
    47401 ccttgacata atttaagtat ttgagcgata agacctttat ttttgcaagc gatagaacca
    47461 attctagcac cacaagcact gtatctctta gaaacactgt ctattattat aactctatcc
    47521 tgtacaccct ctaagttacc acagcttgta tactcaagcc catcataaac aaattctcta
    47581 taaacttcgt ctgctattat ccataaatct ttttctttag ctatttcagc taggatatta
    47641 agttcttctt ttgtataaat tgcaccagtt ggatttcctg ggttagaaag aattatagcc
    47701 ttagttttat catctacttt agacaatatt tcttctttag atggaaggtg gaaaccgttt
    47761 tctgcctttg ttgtaacagc attaacttct acgtttacag attgaccaaa tccattgtag
    47821 tttgtataga atggttctgg aactaataga ttatctcctg ggtcacaaac tgccatcata
    47881 gtgaaaagta aagcttcact accaccgtta gttactaata attcatcttt ttcaaaattc
    47941 atatcgtaag ttttatagta attttgaagt gcttctaata attctggaat accttctgat
    48001 gtagcatatt ctaagacttc gctatcaaag tttttaacag catcaaaaaa tcctttagga
    48061 gtttttatgt ctggttgtcc tatgtttaag tgatatactt tgattccttt atcttttgct
    48121 gcttgtgcaa atggaacaag ttttctgatt ggagaggctt gcattgcact aactctgttt
    48181 gaataattca ttttaatacc ccctatttaa tttaaatacg tatattgtat tatgtaaatt
    48241 gtatgtttga atacaagaca tctttacaag ataataataa catacttatg ggtaaaaatc
    48301 atcaaaaaaa attaatatag ctttaaaata aacttttaac aaattatagt aaaataccca
    48361 tgtttatgcc actaaattat gtattcataa attaatttat agtaagataa tattctgatg
    48421 tactgatagt gattttttag ttataatctt tcagctatag acaaaatata taataataaa
    48481 tatgcaaaat tataaaaaat attataaatt tatttaacag atacgataaa tatcatataa
    48541 ttatattaaa ggctattaac taagaggtgt taattatgaa agaattaaga atagatccaa
    48601 ttaccaatga tgttgttata tttgctacag ataggttaaa aagaccttta gatacagcag
    48661 atatcccaaa tgaagatgaa gaaactaatg aatatgatga agaatgccca ttttgcaaag
    48721 gaaatgaagc tcatgcaact gatgcattat ttgaaataga tagtgaagaa ggatggcttg
    48781 taaaatctgt ctacaataaa tttcctataa ttgatgatat ggcaagagat gtatatggtg
    48841 ttcatgaagt aatgatagag agcgataaac ataatcgtag tttttataac atgagtcaaa
    48901 aagaatttga agatgttttt tttatgtata gaaatagatt tagagatttg tcgaaagatg
    48961 ataaagtaga gtatgttagt atcttcaaaa acttcttgag aaaggcagga gcatctttga
    49021 tgcatccaca tgctcaaata ttgtctatgt cttttattcc acctgaaatt acgaatgaac
    49081 tgttggtttc aaaggagtat tatgacagca ataaaagctc tttgtatgat gatttaatag
    49141 aaaatgaaat aagtttagat agaagagttg tttataatgg agaagctttt ttggtactta
    49201 taccatatgc aaccaagtat agtggagaag taagaataat atttaaagat aaaatagagt
    49261 ttggtgagtt aaatgataat aatatacagg agctttctac aatatttgaa aagttgttta
    49321 agaagttata caatatcaat ggatatatgc catttaattt atgtattcac actcatccaa
    49381 caaagattga aacaaaatcg tattttaatg ttcatatgca cataatccca agaaaatata
    49441 attttggagg atttgaactt ggaacaaata tgtatgtttc ttcaatgagc cctgaagatt
    49501 taacaaaaaa gttaaaaatt gattaaaaca tatattttta ggtattaagt aaatagtaat
    49561 tttagatgaa attatagtat agcattgtta aaataaaagt aaattaagaa tttacaataa
    49621 aattgttgaa aagcccccat ctttagctat ggggagtgtg tcaatgttta gatgaggagt
    49681 ataagtatga aatttatatc atggaatgtt aatggaataa gagcttgtgt tgggaaagga
    49741 tttttagact ttttcaaaga agtagatgca gatatatttt gtttacaaga aactaaattg
    49801 caagaagggc aaatagagtt agatttacca ggatattttc aatattggaa ttatgctgag
    49861 agaaagggtt attcaggtac tgctatattt acaaaaaaag agcctttaaa agtaatgtat
    49921 ggaataaata tagaagaaca tgacaaagag ggtagagtga taactctaga gtttgaagat
    49981 ttttactttg ttacagtata tacgccaaat tctcaatctg aattaaagag actagaatat
    50041 agaactagat gggaagacga ctttatagat tatttaacca aactagataa tcataagcct
    50101 gtaattgtgt gtggagatat gaacgttgca cataaagaaa tagatttaaa aaatcctaaa
    50161 aataatatga aaaatgcagg ttttactaaa gaagaaagag agaagttttc taaattatta
    50221 gacagtgggt ttatagacac atatagatat tttaatcctg ataaagaagg tgtttatagt
    50281 tggtggtcgt atagatttaa tgcaagaaaa aataatgcag ggtggagaat agattatttt
    50341 tgtgcatcca aaaaattaga agatagatta gtaagtgcgg atatacacac tgaaatatta
    50401 ggttcagacc attgtccagt tgaattagag ataaaataaa aattaattat gataaaaatt
    50461 gggctgtaga tataaattta aagttaacta gttttttaat ctacatgcct attttttatc
    50521 taaataaact ctatttgttt tttctttgct taacattttc taatgtaaat tgatttgttt
    50581 tccacatatt ataagctaag ttaatgattt ttttctcgtc tttagttaat tcacgattta
    50641 aaattttcct aaattcatca aataacttat cataatcaac tctttttttg ttagaatatc
    50701 ttatagtttt aggttttata ctcgtataaa gatatatatc tgggactata cttggattgt
    50761 atatcttaat aaaaacttta gcagtataat atgcatcatt taaagcattg tgataagaca
    50821 ttttttcatc aagttccaat atagaaatag cattttgtag gccaatgctt tttcctgctg
    50881 gattattgaa atatttagat gcatgttgtt gtatattaat ataagattta ggtaaggaat
    50941 ttgatggtag cttgtgatag ttgatattcc tgtataattc cttcaaatct ccagctcccc
    51001 aaacacataa tatagggtct tgtgatgata taaattttat aaaattgttg tagacctcag
    51061 ggaaagtagg cgaatcatta aaatcagaac ttttaatatt agtcatacga ctaataaagg
    51121 gatgtatgtc tttataaatt gtaggtttta catagctatt aaacgtatct attatattga
    51181 atttagaatc caatttaatt gctcctattt gtataatttc aaaaggacac ttttcattgg
    51241 atacagtttt attgagtttt ttatcaaatc cttggttaaa ttccaaatcg aaaattatgt
    51301 attccataaa gtttatctcc taaatttttt tattatttct tttactatag attatattat
    51361 aaataaaatt aaattttcaa ataaaaatac ttttatttta gtatacccca tatttgtaaa
    51421 aatgttaaac tattgtatgt ataatatttt ttccaataaa aaatattgcg aactttttat
    51481 acttgatata taataatata aataaaaaca cgaataaaac acgaataatt tgcaagaaaa
    51541 attcataaat gtaaggaaaa tgaaatttat cttgtattta acaaaataaa atatacataa
    51601 aaattatggg agagtggaaa aatggatagt ctattatacg taatagagaa agataaacac
    51661 actaatgaag aattaagaga aatcctaaaa agcaataaaa atataagatt tgtttcctta
    51721 atgggggttg atttaggagg aaatgcaaca gatgaaaaga ttcctgtaga attatttttg
    51781 gaggatatag ataaattttt ggaatcagct atacagactg atggttcaag tgttgagctt
    51841 tataatatag ctacattaaa taatgcgaaa gtagatttaa tgccagataa aagttgtcat
    51901 tggtatgtag attacaatat ggaatatata gacgaagaag taggtctacc tgttggaaca
    51961 ttaaagatac ctgcattttt aatacatgat aataaaaaag tatgttctag aggagtactt
    52021 caaaaagcag ataagtactt taaaaagtca atgtatgaaa tatttagaga atatccacat
    52081 gtaataaata acataggaat agattcagta gatgatatag aggaaataat gttaacagca
    52141 gctacagaac ttgagttctg ggttaatact cctgaggata aagctgactt agagaaatta
    52201 tatgtatcac aaagcctaaa agagcaatac tggaaaagaa ctcatggtat tataagaact
    52261 tgtttagaga aaagtttaat tatacttcaa aagctaggtg taaaccctga aatggctcac
    52321 aaagaggtcg gaggaataca aagttctatt agtatagatg gaagaacaaa ccatgctatg
    52381 gagcaattag aggtttcttg gaagttttct acaccgttac aagcagcaga caatgaatta
    52441 ttagtaagag atgttataga ggatgtattt acatcacatg gactagaagt tacatttaaa
    52501 gcaaaaccaa ttcatggagt agcaggaagt ggtggacata cacatgtagg agtaagtgcg
    52561 aagttaaagg atggaagtat aaaaaatcta tttgcgccaa aagatttaaa agaagattat
    52621 ctaagtgaac taggatatgg ggctcttatg ggacttcttt acaattatga agtattaaac
    52681 ccaattgtaa cagcttctaa tgatggattt aatagattag ttcctggatt tgaagctcct
    52741 gtatgcatag ttacatcttt aggtcatagt tatgagattc catcaagaaa taggtcagtg
    52801 cttgttggat taataagaga tatgaaaaat ccaaagactg taagatttga gttaaggtca
    52861 ccaagccctc tatctaacac gtatttagta atagcaggat gttatcaaac aatgttggat
    52921 ggtataaaag cagcagctaa gagtggtctt agtacaaaag aacttgaaaa agagttatct
    52981 aaaaatgtag gtgaagaaag tttctattta gaaaaagata gagcatatag agatgaaaat
    53041 gatgtatttg aacattatag tttggaagaa agaaatgcaa gatttggaat accaccggct
    53101 acagtttatg aaaatatgaa aaacttagaa atatatgctt caaaattaaa atctttaaaa
    53161 caaggtgatg tatttactga tagtataata gaatcattta agattggagc tatagataag
    53221 tggcaaaaga aattaaaaac tagaataata gaggctggga ttcaaaaaat aagaagtatt
    53281 gtcaagatac acacaaaaga aaatatggat gcgcttgatg aggtagtttg gaactcaata
    53341 tcggatttaa agttcaatat tatgaaggat actttaacta gagaatcact attcactaga
    53401 gtgagagaag ctatagaaaa taaggattac cagactgctt cagatttaca aatagaatta
    53461 aagagaagta tggaagaaat acaacaactg tatatgcagt acaagaaaaa tatatactaa
    53521 tataacaatt tgattttgat atgataaagg caatcaagta ttattaaaca ctatgagtaa
    53581 attctcaagt gtaaaagcat acttgattgc ctttaatttt atgtttgaaa ttttttaata
    53641 gtacaattta actgtaatga ttatctatta attctgatat ataatcagca attttctctg
    53701 gatgaatgac tgttttaaat tcttttaaat tttttttcat aaagtttatt atagaatcaa
    53761 attcatttga tactaattta taaaatatgt ttttaaggtc taggtcatca ctataaatca
    53821 tgccaagacc cttatctatt ataaatttag cgttttcaat ttcttgtcca acttttggca
    53881 tttttacgag tactggagtt tgtgattgaa tagcttcaaa tatagttgct cctccaggct
    53941 tagttagcat taggtcacat tctcttatta gattatacat gtcgtttaca aatccaagta
    54001 ctttaatatt ttttagaggt tttttatgag ttagattgtc atataatttt ttgtttttac
    54061 cagtaactat tgtaatttct atagaattac tatgttcttc aataaattca tctatccaat
    54121 gcataaaatc ctcatctata tcaaatagac ctcttcctcc acctagaagt aatagtctat
    54181 atttatcaca acatagttct ttactttcaa tttgaaatct cttatcaaca ggaactccag
    54241 taacctttat ggagtctgga tttattcctt tctgaaaaaa tctatttttt atttcaggag
    54301 aaggaacaaa atacaaatct gtattttcaa aaacccattc catactatct acaacatctg
    54361 ttataactgt aagtgtaggt atatttatat caggattttt tatcttaaag ttatttacac
    54421 aagcagcagc tagtggaaat gtagagataa tcaaatctgg atttatatca gcaatatact
    54481 cagaaagttt aggtgtatat aatttatgag ataaatcatg tcttgaatca aacgatttct
    54541 taaagtaata atagtaatta tataatccag gagtatattt tgtattgttt tcataaagtt
    54601 ttaccatagg cttattcatc atgggaatac ttgcatttat aaagttttgt ataacaacat
    54661 tatagttact atatttttca ataatacatt ctttgactgc ttttgcagca cttatatgtc
    54721 cagcaccaaa ttgagcagtt aggattaata ttgttttatc atctttcatt ttagccaatt
    54781 tatcaccact ttcataaaat atatctgatt agagtattat acatttatag aaggatttaa
    54841 tcaacaagaa tatttaaaaa taactattgt gcagaacata gtactgtgtt attatgagga
    54901 tataagaagt tttaacatag taccttgtaa aacacaatac tgtgtattta aagaaaggat
    54961 ggagtatatg aacacacaat ttagaaaggg tgttttagaa atttgtgtac ttgctctcat
    55021 atctaagaaa gatatgtatg gatatgagat agtacataac atctctaaag taatagatgt
    55081 aaatgaagga acaatatatc ctattctaag aagacttaca aaagatttgt atttcgaaac
    55141 atatatacta gaatctaatg aaggtcctgc tagaaaatac tatagaataa cttcactagg
    55201 aaaagagaat ttatccaatc tgatggaaga gtggagagaa tttgtaaagg ctgttgagat
    55261 tttaataagt gaagatgagg gaggtaatga acttgaataa aactgagttt cttgaaattt
    55321 taaaggatta tctaaaaaaa gatttttctg agagcgaagt taatgatata ttgagagatt
    55381 atgaagagta tttcgtagat ggtcttatag agggaaagag tgatatggag ataatagcat
    55441 cacttggctc accaaaatct atagcaaaag aacttatctt acaaactaaa gaaaaaaata
    55501 ttgaagagga aaaaataata aatacagatg ttttgaatga taagtttagt aaatttaaat
    55561 cagatatgaa aaaaggttac tctatcttaa aagatgaagc aaaagaaaag tatagtaaat
    55621 caaaagaaaa tatagatgga aaaataatga atacagacat attaaatggt aaatttagta
    55681 gatttaaatc aaatgtaaaa aaggattatt ctatcttgaa agatgaagct aaggaaaagt
    55741 acaataaatc aaaagaaaat ataaatgata aacttacacc aagtatagaa gaaggtgtag
    55801 gaggattatc cagacaagtt ataaaggggt tattagtagt gttatcttta tttctaattg
    55861 ttccagcatt tgcgtttgta tgtgctattg tatctgctac tattgcatta gtatgctcat
    55921 taattgcttt tgttatatta attccatttg tgattaaatt tatagcaatg gttccagatg
    55981 ttatgatgtt ttgtattttc gcaaccatag catttgtagg gcttgagata cttgcatggc
    56041 aaattttatt atttgccata aaactaggta aacaattact aaataattat atacattgga
    56101 taaagagaaa aaatatttat ataaaggcaa atgaaaagat ggaaaaaaga aaaaaagcta
    56161 ctttaaatga tgaagatttg aataaagaca ttaatgaaaa ttcatataat aatgattact
    56221 acaatagaga agaagaaagc ataaaagaaa ctgatgaata taatgatgat agtataaatt
    56281 ttgaggcgat atataaggac cttgaagata tagaaaataa gaaaggagaa gataaacatg
    56341 aataaaaaac ttgctatatt tgctgcagtt ttattggtaa taggtataat agggacaaca
    56401 tggtcaggga ttttagttat gccaagccta ataaactttg gtttagaaaa ggaagctgaa
    56461 ttaaacaaag aaaacaaact ttatcaagaa aaagtaaata ttgacaagtt agatatagct
    56521 gttgacaata taaatgttat gataagaaaa agttcaaatg aagatgtgcg tgtgacaaca
    56581 agagggaata atgaatttta caaatacagt gtaaccctac aagataagac tttagttgta
    56641 aaaggcgaaa gaaaatctga aaataaaatt aaaaagatta gaagtttcga ccacttatta
    56701 aatacatcta taaatagtat gttttcacgt gattatagag aaattagaat atatgtacca
    56761 aataatgtag atataaatgc atcaagtata agttcgtact tatttatatc tgatgatgta
    56821 gattcaaata atataacata taaaacttct tctggaggtt ttagcagagc tataacagaa
    56881 aataaagtaa atagattaga aaacttaaac ttaatatcta atagcaatct tcatctatca
    56941 acaaagtcaa ttttaggcgt taaaaatgta aacatagaat cagagtcatt ggatatatct
    57001 tcagagaatg gagatgtatt tatcaataat atagaagagt atatacctga aaatgtaaat
    57061 ataaaagaga aaataggtag aaattcaaat tatgaaagtg aattttactt gtactctgat
    57121 atgccaattg ctaagttcct ggacatagaa gttccaaatt ctaaggtaag acttgatata
    57181 ccagtaaata agtataaatt taattgtgat ataaagtcaa aagaagcaat tgaagaattt
    57241 aataatgatg aagaatatga caatgattca tatgattatg aacataatga agaacatagc
    57301 aacaagtata gaaatacgag ggaaataaaa gggcttttga ataaaaatct gtcgaattta
    57361 gagaaagaat atactataaa aataaattca aatagcttag aattgtaaat aatttactat
    57421 aataatttat aaattgattt aaaagtataa aaatataatc tatactatgg tataataagt
    57481 atcgaaagag aattaggaga gggatactta tgaataaaat agcagtaagt tttttaataa
    57541 ttgctactac tttattgagt acggcatgta tggattatag catcagtgca gttgagctag
    57601 ttgattctaa agaatcagca gttgtaaaga aggatgaaga tgcaaaagaa gaaacaacta
    57661 gtaaaatgat taattctaaa aaaactacta aaatacctat agagataatt tcaaaggatg
    57721 agaaaatagt aaagtactta cagattgatg aagaaagttc acttaaggat aaactgcgat
    57781 taatcttaga tacattatca aatgagtatt ttaatggttt agacatggaa gtagaagtta
    57841 aggaaaaaga taatcttgtt aaaataaact tgatagaacc agataaaaaa agtagagttt
    57901 cttggaaaga tgattatttg aatgaacaaa atataatata tactataaat aatattatta
    57961 aaaatgtcat ccaagaagaa gataatagca tttggattga agaagtagag atatactaca
    58021 atggaaaatt aatagaatta agataaaaaa agatgtgttt ttaacacatc tttttttgtt
    58081 ttttaattct tataaaatcc ttatatttat gatttagaat gattcttgta aataagtttg
    58141 acaaaagaaa ggatgaaaat tatgagtaat tatattttgc aggtaaaagg agtttctaaa
    58201 aaatttaagg agcagatggc agttgataac atttcactag caattaaatc taattctata
    58261 tatggattat taggaccaaa tggagctggg aaatctactt tattaaaaat gataactggg
    58321 attttaaatc caacttcagg aaaaataatg tttgagggac atagatggac tagaaaggat
    58381 ttatcaaata ttggttctct tattgagtct cctgctatat atgagaattt aacagcacga
    58441 gaaaatttaa aagtccatac tactttactt ggtcttcctg actctcgcat tgaagaagtt
    58501 ttagagactg tagatttaag agataccttt aaaaagcgtt caggtcaatt ctctctaggc
    58561 atgaagcaac gccttggtat tgctattgca ttattaaatc acccaaaact tttaatctta
    58621 gatgagccta caaatggtct tgaccctatt ggtattgaag aacttagaga attgattcgt
    58681 tcttttccaa aacaaggaat tacagttatt ttatcaagcc atatcttgtc tgaagttgaa
    58741 caagttgtag atgaaatagg aataatttct aatggagtaa tgggttatca aggagaagta
    58801 tcaaaagaac aaaacttaga agaacttttt atgaaagttg tagctgaaaa tagaaagaga
    58861 gggcagtaat atggaaagat ttattagagt ggaatttcaa aagatgaaag gaacttttgg
    58921 aatgaagttg atatggatag ttccagtgtt ttgtatactt ttgtgtggac taggtggagg
    58981 gcaaaatgct gctttcaact ggtggtatac gacatttctt ccaggagcat taacattaat
    59041 atcttcttta atagtattaa aagatgaaaa aagtaaatat cgtggtattt taccattgcc
    59101 aattgaaatg aaagacgttt ggactggaaa aatgatttac tgtattattt gttgttttat
    59161 agcatcaatt atttctgcaa taggtgtagt ttttgttggg ataatctata aagaaactat
    59221 ttcaataggt caaaatatca ttgcaagtat attaattatt ttactattta tgtggcagat
    59281 tcctctttgt atgtttttag cttcaaaatt tggtatgttt gttacagttc taacaaatat
    59341 gttctttact attgttggag ttgctgtttt ctcaactaca gaaatgtggt atatgtttcc
    59401 atattcgatt ccttcaagat taatggctgt tgtattgcat attctgccaa atggtcttcc
    59461 tgtaccagag ggaagtgaat ttttaaataa tgctgttatt ttgccaggag tattaatatc
    59521 tattgtttta tttgtattat ttgctttagc tacaagggtt tggttcaata atagagaggt
    59581 gaagtaatat gagacagtta ataagaatat tttgttctga tttaataaaa ttaaagagaa
    59641 attttataat actaatgcac atttgcattg ctttaatggg gatgggattg tttttatggt
    59701 attataaggt ttcaggagct gataatatac taaagatagt agcatattta caagttatag
    59761 caatagcttt tcctttattg tcaagtgttg tgtgttcaat gtttgttgaa caagaatatt
    59821 atgcagggaa ttataaacat atgttaacaa catctaatcc aaaatatctt actcttataa
    59881 gtaaatatat aatcttagta tgtcttggtt ttgtagcaac attagtgtct gtattaggtt
    59941 ttaaatttgg tatatctaag gcttacttta catcaagctt ttatatgatt tcaattctaa
    60001 ttttaattgg gtgtaattta tttgaataca tattacattt attcttaagt ttaaggttcg
    60061 gaaaaggaac atcaattgga gtaggtatag ttgaaatgtt actttcagca ttattattaa
    60121 ctggattggg ttctggtatt tggcagtatt ttccttgtgc atggggtgtt agatttataa
    60181 ctatttggtc gtctttttca agttctaaaa cggttgaata tataaaggtt gaatcaataa
    60241 aagcatatca atcaacaggt ttaatttgtg gttttgtaac aattttagca tttgtaattt
    60301 tatgtatttg gttttctaaa tgggaaggta aaaaatcaga agaatagagt aaaaattgac
    60361 tgtttattta tattattatg ttaaaatata acttagcaat aaaaagatat aaaataatca
    60421 aataaaaaac tggttgggta ttaattatga attaattaaa ctattggagg acagggtaca
    60481 aatgcttgga ggtaaaaaat ctaacaacaa aaaagttgaa aagttaagaa ctatttttat
    60541 aaaatattta tctttattct ttattatgac aatttcaatt gtactccttt taatgttatc
    60601 attttctgta ttattatcat caggtgtaat tttaccagct aattatgcag aaaaacaatt
    60661 taataaatat aaagaacaga ttatttcaag tgaaaaagta actgaagata taattccaag
    60721 tatatatgag tatggggttt atactttgga tgggaattta atttcaggga cctttaataa
    60781 aaaagaatca aaagaagtat ggaatttaat gagagatatt gaagaaaggc atgcatattc
    60841 agaaagttat ataaagtttt ttaagaaaga tgaagttttt ataataaaat acaaaatagt
    60901 atcagagtat agttctccaa ttttaagagc atatttacca aaaccagaga cattgggtat
    60961 gattattttt agtataatat tttttattga aattgttata ttatcaaaag tatttggcaa
    61021 gaaatttaat attgaaatgg agcttttaaa aaatactaca gaaaaaatag aacaacagga
    61081 tttagatttt gttgtagaat ctagtaaaat ttatgaaatt aataatgtcc ttttttctat
    61141 ggataaaatg aagttggcgt taaaagattc tttagaaaaa caatggatgc tagaagaaaa
    61201 tagaaaggaa cagatatctg cactggcaca tgatataaaa acaccactaa caattataca
    61261 tggaaatact gacttattaa ttgaaataaa tgaaaatcct gagttattag aatacatgga
    61321 atatatctca aagggtgtca ctcaaataga aaaatatatt aatactttaa tagatatatc
    61381 aaaaacagag aatggctata tattaaataa agaagcaatc agtatgagtg agtttataaa
    61441 agatgtttta gtgcaaatag aagcattagc tagtactaaa aacttagatg tggagtttta
    61501 taaacaagat aatttgccta aaaatataac tatagataga gaactgttat tcagagctat
    61561 tatgaatata atttcaaatg ctatagatta ttcaccaagc cagagtaaac tatacataag
    61621 tgtatcagta cataataaat acttaaaatt tgtagttaca gattgtggaa gtggtttttc
    61681 taaagcagat ttgtcaaaag ctacagaaca gttttataca ggagatttga gtagaaattc
    61741 taaatctcat tatggaatgg gactctatat tgtaaataat attgtacaaa aacataatgg
    61801 gactttatgt attgaaaatt ctgtgaaaac tggtggtgct atggtaacaa ttgaaatacc
    61861 aatagtttag taaataatat ccattttgta aatacctaaa ttttatgatt tataggtatt
    61921 tataaaatgg atagtttttt tgattttata tattggttaa atctgataaa aagttttcaa
    61981 acataactcc atcatttcta tcagtatcaa attgagatgt gtaatttata gttttatata
    62041 taaaatctgt tgcagtatta accgctactc ccaaatcatg tttatttact aacataccac
    62101 aaagtataga tgtgaatata tctccagttc cactgtaaga acaattatta tatttgactg
    62161 atgtaaagaa tacatggtca ttatctctat tgtaagccaa atttaatata ttatcatctt
    62221 ctaatatacc agtaataacg actttgctag gtcccaaatc agatacagat tttgcaatat
    62281 atataagctc atctctatta taatcgcttt tggtataatc atttccagtt aagaaacaag
    62341 cttctgttaa attaggagta gttaaatctg aatgtttaac taattcttta atttcctgtc
    62401 tcatttcttc tgtgaatatt ggatacataa ctccattatc acccatcaca gggtcaacta
    62461 cgataaaagc attgggataa tcgtttataa aattagccac tatctcaatt tgatgctttg
    62521 aacccaaaaa accactatat atactatcaa aattgacttt taaatttttc caaacatttg
    62581 aatattttac catttcatca gtaaagtcta agaaagtaaa ttcaggataa ccagtttgac
    62641 ttgaaagtat ggccgttgga aatggacaac attgaacctt taatgctgaa agtataggta
    62701 tagcaaccga taatgagcat tttcctattc cagataagtc attaattgct gcgacttttt
    62761 tttgcataaa aaaccctcca ttagtatgta ttaattataa tgataccaaa atatttatat
    62821 acatatatat tactataaaa aaaagaaaaa agtagattat aaaataaaaa attgtaaaaa
    62881 aaacagtgta ttgatacaat taatttttaa tattggacat aataaaatag agaattatta
    62941 aatatgaaaa ttactatatg tgacagaata ttctttgtaa tttgatttgt aaggtgtact
    63001 atttgggagg tgtagctttg aaaatactga aaaataagaa taagtacatc aaaggtataa
    63061 tggcatttgt atttttgtct ttagctacta cagcaggtgt atttttaatc aatgatttaa
    63121 gtatgaaaaa tatggaagaa aaagtcatgg aatctgcaaa tgttgaaggt gatgatgtaa
    63181 aagaccaata taaactattg cttgaaaact tatttgacta cagaaataag gcaatattag
    63241 agaaaaatga aaatatttta aaaggtcttt atgaaacgga taaaaaattt ggtctgtggg
    63301 cttatgaaaa tgaagtaaaa aagatgaaat atttagaaaa ctggtctagt aaacaaggtg
    63361 taaaatttaa agacataaag acaagggtta agattaaaaa aataaaggaa aaagaaaaag
    63421 acctctatgg tataatctgt tcagtatcca ctgaatataa gtattcatat gaaaatcaaa
    63481 aggacaaaga aaatgccttt agaataggta catatcatta tctaaatgta aaaattatag
    63541 ataatcaata tgtcataact aaagagtggt atacagaccc ttttgcagac tctttaaatt
    63601 tagaaaatat aaaatctgat gatataagaa attatattgg tcaacaagat ggtgtagagt
    63661 tacagcttac agatgaacaa aaaaaagcta tagattatgc acataaatac tgtggagccg
    63721 cagctgatga agaacatggt atgaaattta atccaaacta tagggattat aatccagaag
    63781 gtggagattg tgcaaacttt gcatcacaga taatgtttga aagtggaagg ttcaaaaaga
    63841 actcaatttg gaattatagc gagagagcag gtacaaaagc atgggtaaat gcacaagctt
    63901 ttaaaaatta tgcactgtat agtggaagag gttcattaat tgctaaaggt tcctatgaag
    63961 aagtatacaa agaagcttat aatttacgtc caggggattt tgttggttat gagaagaaag
    64021 gcagaataac acatgtttcg acagtgacag gattagactc aagaggatat cctttggtaa
    64081 cttgccataa tactgataga atgttggtgc catgggattt aggctggagt gataagtcca
    64141 taagattcca tataataaaa ataaattatt aaaaaattat aaagaaaaat aaagtgtaaa
    64201 agataataat catttaaaat tataaagtaa aaaaattgat tattaatgta taaaaataat
    64261 aataattgac aaacatagct atatatgata gagtagtaat aaggttacag aaaagagtag
    64321 ctaaatattt gtgttttttt aggaggattt attaatgaaa actggtgtag ttaaatggtt
    64381 taacaacgaa aaaggatttg gattcatatc tgtagaaggt gaaggagatg ttttcgtaca
    64441 tttctcagct ataactggag atggatacaa aacattagaa gaaggacaaa gcgttgagtt
    64501 cgaagtagta gatggagcta aaggtcctca agctgctaac gtagttagat tataattaaa
    64561 aattcatata tattagtgtt ctagtaatat gagagataag ccgctaatca tttagcggtt
    64621 ttttcagttt ttgtaaataa aattaatagc aaaataaatt aaaattatta aaataaaatc
    64681 attaataatg agataaatat gttaaaatta atagtgtaga gtaaaattat tatggctaag
    64741 tttaagtcaa ttaattttta gtaaattttc taaaaagaga ataagtgata cttatagacc
    64801 atataaaaat attaataaag cataataaga ttaaaatgag caaaggaaaa acgcttgtta
    64861 cagcgtatag tattaagaat acttagaaaa taagaaagga gaaagttact gcaaaaaagt
    64921 aacattataa atattgagta atatttataa gttttcagta acggtgggta gtgtaatgaa
    64981 catattattc tttctaacac caaaaagtga agtagcatat atatatgaag actacactat
    65041 aaggcaagcc ctagaaaaaa tggaatatca caaatactct gcaattccaa taataagtaa
    65101 ggatggtaaa tatgttggaa ctataacaga aggtgatttt ttatggacat taaaaaatga
    65161 tttaaatctg gacttaaagg gattggaaga tgttccagtg acagatataa atagaaaaat
    65221 ggataattct cctgtttcca tcaacgcaga tattgaagat ttagttataa aatctttaaa
    65281 tcaaaatttc attcctgtta tagatgacca agacacattt attgggataa taaaaagaag
    65341 agatgtaata ggttactgtt atgagataat acgtggatat aaaaatttag ctaatgataa
    65401 ttaaaaatct tatttaaact tttaagctga gttctttaca ataaaaaaat gataatatga
    65461 aactgaatat ttaaacttta atataaaaag ctgtcttttc taataaatat tgtgaaaaga
    65521 cagctttttt atatatgacc ttatgattaa tcgttgtatt ttacaaggtt tccgtactta
    65581 gcattaagag catttatctt atttccataa acttcatttt gtttttgata taataaactt
    65641 ttttcaactt cagccttaac ttcatcaaat gtagactctg tactttcttg aaggtcttct
    65701 aattttatta tatggtatcc aaattgagtt ttaactggtt cacttacttc acctttagcc
    65761 atagagaaca cagcttcttc aaattcagga accatttgtc ctctaccgaa tgttcctaaa
    65821 tcaccaccca tatctttaga tgggcaagaa gaatgtttaa gagctgcatc ttcaaaagat
    65881 atttcttctg attttatttg agcaagtatt tctttagctt tttcatcact atctactaat
    65941 atatgtttag cagttgcact ttctggttta gagaaagagc tcttattagc ttcaaagaat
    66001 tttacttttt catcttctgt tacattaaca ctagataaaa ctttatttat agcaaattgc
    66061 tttaatacat tttcttctat ctttttcatt tctaatttaa atatttcttc ttcatctaat
    66121 ttttcttctt tagcatctaa gaagaataat tcttgattca ctaaatcatt taataagtgt
    66181 ttttttcctt cttcagtttt aaattgcata gcttgataag ggtctaatga ttttaatgca
    66241 ttttcaatat caatattagt tatctctttt tctccaactg ttgctaaaac ttttttttcc
    66301 ataatagtta actccttcat agtgtatttc attattaata taagactacc atcttaaata
    66361 atatcagaaa ataatgtaga tgtctaatat ttaaagccat attaagtcaa atagaattaa
    66421 atttagtaga acaaagttat tacaattaat aaaagtgagc tcataatacc cactacacct
    66481 tcgtcatctt taaatttaag agtttttact attgaataat aaaggtatat tccagtaaaa
    66541 ataatcttta aatacaagaa aaataaactc tttgggtata ttaaaaagct aaaaagtgta
    66601 aatataaaag caagtagagt acttatcctg taaaagaaat ctatttgtgt aatgtcttta
    66661 tattttatca taaacaccga atataaaatg atgaaaacaa ttgcgacgat tgcatatatg
    66721 gtaaataaca tattttcaag agtaccattt gaaattacta tccacttata ccacataatt
    66781 ggccaaaata atagtgctaa gaacttaaaa taattatcaa acaacttttc tttatacatt
    66841 catcaaacaa cctttcttaa caaaagcata tatttgtttt tagaatttta aataatatga
    66901 tatcattatt atatattaat attgaattta tagaaaccaa aatttgttaa aataaatata
    66961 tagattttac tgttaagcca gttaaaatta ctactatttt tattatgaaa ttggatcaaa
    67021 tatgtagaaa tacggcaaat tagttaatat taaatattta ttatttccaa gttgtaaaga
    67081 ctgttttttt aatgatagaa attctaatct tttttgaaag aaagtaatat ccacattaag
    67141 tatgtctgcc atttcataaa cgcaagtgat gccagagtta attatgttta ttatatcttc
    67201 ttcagtaatt aagaactcac aagcccattt taaggcttta ttttcgcact tatctataat
    67261 aatttttgta taataatcgt tataagagga tacatagtat ccaaggctag tgaaatgatg
    67321 tccaagttct tcagctaaga tggatgtcaa tttttttgag ttttgtttta aattactgag
    67381 taatgatata attttaatac catgtttgtt tatatatagt ccttctaaat cacctgcaat
    67441 ataagtggta taatgaatta ttatctcttc ttgagaagct aattcaaaaa gcttatccaa
    67501 attattcata aaaatccccc taaaatagaa tgtatgtttg cctttgaatt atattaaaag
    67561 agcagaaaaa tagactgctc atcatatggt ttattttttt ttatatttat ttagtaaaaa
    67621 ttctatataa tcattaagtt gttcttgtgc ttcttcaggt aactcttcat gtggattttt
    67681 tctatgtgca gctactgtat caatattttc cttaactaag gttcttccaa gaaggtaatc
    67741 aactgataca ttaaatacat cagccaattt atttaaaatg tgttcatcag gaaatctgtt
    67801 ttctgtttca tagtacccta agactctttg ggaaacgcct actttttctc caagttctct
    67861 ttgagtcaat ccaaattcct ttctaagttc tcttaatctt ttggcaaaca ttataacacc
    67921 accttatgta tagattataa caaattgttc taaaaaataa aactaataaa atataaaaga
    67981 atattttttt ctaaaatcta ttgataaaga acaaataatt ctatataatc taagtgagga
    68041 agaacaaaat attcttaata gtaatggagg tataaaacaa tgtttaaaaa taacttgaaa
    68101 tattatagaa aatgcaaagg tatgacacaa attcaacttg ccagaaaggc tggaattaca
    68161 aatgattata tatctcaaat agaaagaggc ataaaaaatc ctggacttct tatggctaag
    68221 aagatttcta gtattttaga acaaaatata gaagaagttt tttttataca gttatagaac
    68281 aatatgttct tgaaggttgt gagattagta aaaaactgtg cactaaagag attattgtaa
    68341 atttgaagct aataataagt atataaaaaa ggggaagtac tatggaaaac aaaaaagata
    68401 tattatttaa agaaacagat aaaagattac ataattataa gtatttggat ataaagataa
    68461 agaatattaa cttggacata aaaagatgtg agaatgaata ctctggatgt ggagcaatgg
    68521 tatatacaga aaagactagt aacacatata acataagctc ttctgtggaa aatgaggtgt
    68581 taaaaagaga ggaaagatta agaaaattaa aaatggaaaa agaagatata gaaatagaaa
    68641 aagagaagat agaaaatgct ctaacgtgtc taaatgatat agaaatggaa ttttttaatc
    68701 ttttttataa tagtaagaca aaaaacaata tgacatatat ttctatgaaa ctacacttag
    68761 atagaacatc ttgctacaat ttaaagaaaa agatgatatt taaattgagt gagatattat
    68821 aaaaaatagg acaattttac aacactttat atacaccatt acaacaatag gcaataaaat
    68881 atgtgagata atgttattgt gaaagaaatc catattgaag gaggtgatag attgaaaaga
    68941 ataatattac ctaaaaatat agaagatact tgacaggaat aaatgagata tatatttaaa
    69001 aatgacttat atcatttata gtaagattat cagattaagc aagaatattt agtgatagtg
    69061 tggtgattat ttgcttaaat acaaggaaat attagaaaca attattgaga ttctcaaaaa
    69121 aaactttact gaaagtattt ttattgatga tgaaagtgtg caaggctctg aagggtcttg
    69181 tttttttgta agtatactat cagttatttg tacacctgta atgttaaata cgaataacaa
    69241 agatattgtt atctctataa aatacttacc aaaaccacag tcaaagagta ttagaatgta
    69301 tgaagtttca gatgaattaa ataagttatt caacagaaat ataaaggtaa cagacagaaa
    69361 attaaatata acaaagctag aacaaagtat taaaaaagaa gagtcaattt atgtattgaa
    69421 ctttacattt acactaaact atctggatag tgtatatgaa gaagatgtag tatatgaaaa
    69481 tatggaagaa atcaatttaa atttaggaga gtgatagtat ggctatagga ttaccaagta
    69541 tcaacatatc atttaaggag ctagctacaa ctgttaaaga acgttcagct agaggaataa
    69601 ttgcaatggt acttaaagat gctaaggcac taggtcttaa tgaaatacat gaaaaagagg
    69661 atataccagt tgatttatct gctgaaaata aagaatatat aaatttagct ttgatgggaa
    69721 atgttaacac tccaaataag ttattagttt atgtaataga aggagaagca gatattcaaa
    69781 ctgcattaga ttttttagag actaaggaat ttaattatct atgtatgcca aaagcagtag
    69841 aagctgataa gactgctata aaaaattgga taattaaact tagagatata gataaggtta
    69901 aggttaaagc tgtattagga aaagttgtag gaaatcatga agggataatt aattttacta
    69961 cagaagatgt gttagttgga gaaaagaaat acagtgttga tgagtttaca agtagggtgg
    70021 ctggacttat agcaggtaca cctttaagtc aatcagtaac ttatactaaa cttagtgatg
    70081 tagtcgatat acctaagatg acgaaagttg atgcagaatc aaaggttaat aaaggagagc
    70141 ttatacttat taaagaagca ggagctataa gaattgctag aggagtaaat tctttaactg
    70201 aattaacagc agaaaaagga gaaatgttcc agaaaataaa aatagttgac actttagata
    70261 ttatacatag tgacataaga aaggtgataa tagatgacta tataggaaag gttactaaca
    70321 gttatgacaa caaatgttta ttgatagtag ctataaaaag ttatttagaa gaattagaaa
    70381 aatcagcact tatagaatct gattctactg ttgaaataga ttttgaagca caaaaatcgt
    70441 atttaaaatc aaaaggagta gatttatctt atatgacatt acaagaaata aaagaagcta
    70501 acacaggttc taaagtattt ttaaaagcaa aaataaaagt acttgatgct atggaagata
    70561 tagatttatc aatagaaata taggaggatt attaatatgg caaatatgga agctagaaat
    70621 gtaatgagtg gtacttgggg agaactttgg cttgatggaa acaaagtagc agaagtaaag
    70681 aaatttcaag caaagatgga atttacaaaa gaagatatta taatagcagg tcaaatgggt
    70741 actgatacaa agtatatagg atataaagga aaaggctcaa taactctata ccacgttagt
    70801 tcaagaatgc acaagttaat tggagaaaag ataaagagag gttctgaacc taggtttgtt
    70861 gctatatcta aattaaatga cccagattct tatggagcag aaagaatagc agtaaagaat
    70921 atagcatttg atgatttaac tttagctgat tgggaggttg gcgtaaaagg agagatagaa
    70981 gcacctttca catttactga gtatgatttt cttgatataa tttagtttta tatttagttt
    71041 tatatttggt tttatactga tatttagtaa atatatactt aataaattca gatagttaat
    71101 aagtaaaaaa gttagttgat tgaatttgat tgataaagga gcaaataata atgagtgaaa
    71161 atggattatc aaaaaatata aacatagtag atttactttt aaattcagat acagaaaact
    71221 tagaaagacc gagtactata gttgaactta agagattatc aactatattt gggcaggaat
    71281 ttaaagtaat gtgtagagct ttaacaataa gtaaagatga agaaatacaa aatacttgtc
    71341 ttaaaattga tgaaaatatg aaaacggata tagacttacc ggagatgcag atgcttacaa
    71401 ttatagaagg tgtttgtgat ttggatggaa agcttttatt taaaaataag gagctaatgg
    71461 ataaatttaa ggctccaaca ccaaaagaat tagcaagaaa attattatta ccaggtgaaa
    71521 ttaccaactt atatagaata cttcaagatg ttatgggtta tggtaaaaat gcagtgatag
    71581 aagaggtaaa aaactaatag ggacggatac caggactaca ataatgtact attattggaa
    71641 gaaaaaaggt ataagaccgt ccctttttta tgcaatggat aaaggcgaat taaagcttat
    71701 tgaagctttt ttcgccttag aaattgagga agaagttgaa aaaatgaaac atggatatgg
    71761 agtgtgtcct ttgacaggag gtggtatgta atgggaaatg tgagagaaga aggtataaat
    71821 atgtacctta cagataatta cacaccaaaa atgaatcaaa ttatatcagt aactgataat
    71881 tttaggagag caactgtggc tgtttcactt tccactaatg taatggctag tagcataaaa
    71941 aattctattg gaagtgcaag taatagagta aacagtttaa attcctcgtt aagaaaagtt
    72001 caaactactg ctagtagtgt aagttcaact atgacaaaat taagttctag cataaatgct
    72061 gtttcaggag ttattggaag tttaaatgga agtattatga tactagcaat aactatagct
    72121 atgattattg attattttaa taagttgatt caaaagaaaa atgagtttaa ttcaaatatt
    72181 atgattatat taatatttaa agctaaaagt gatgaagtag aaaaaactaa aaataaatta
    72241 cttggaaatt taaaaaagat tggtggcaag gtttggaata tcgtaataaa agcaaaagat
    72301 atgactaaga gagttataag tagtatcttg ggaaaattaa aacaagtaga gaaacgtcct
    72361 tatcaaggaa gtattaatct taaagatatg gtgagtagtg ctatgggtag aattttgcct
    72421 aagttaatgt tgtttaaaaa tactttttgg agtggtgtaa tagctataaa agatatggca
    72481 agtggcatta taagtaaagt atttcccaaa ttgagattat ttgcaggtaa ggtatggagt
    72541 ggtgcaatag ctgtaaagga tatggcaagt ggaatacttg gttcgataaa agggaagata
    72601 tctgatttga caaatggtgc tactataggt gtcgctgtga aaaagggtgt tgatttactt
    72661 ggtcaggaac aaaatcagaa agttgttcta gaaagtgtaa tgaaaagaaa tactggaaaa
    72721 gttaatcaaa tagatgttga tgattattat ggcagtttag taagaatggc aaatgatacg
    72781 ccttttgacc ctgaagatgt tgttgcaatg ggaactaaag ctaaaatgat tagtaatatt
    72841 actggtggca aaaaagaaaa agatataact caagctatgg tagatgttag agctttaaat
    72901 atgaatacaa gtagtgaaca agatgtatca gcagctttct taagtgcagc aaaaggaaac
    72961 atggaatctc ttaatactct ggtaggagaa aattataaaa cttttgatga agcattggaa
    73021 ggcataagtg taaagcagat ggggttagct aaagaaatga gtaatacaat accaggtata
    73081 atatcaggag ctcaaacaag cattaacaat ggcttgaaga gtattgttaa accttttgat
    73141 gatattttag gtcaaggact aaagaaaata aaaactttta tagaaagtgg attaggcaat
    73201 ttagctggct tatctgaaaa aatggctggt aaaataggca atgtaatgaa tggtaagata
    73261 attattggca acaaatatga ccagatgcaa tctagaagtg taaaaaatgg aaaagagttt
    73321 tctgattcta ctcaatatcg aatttctaat gaggctgaaa agcgtaaaat gatggttgaa
    73381 aataagcaag aacgttttga aaatcatgca gcaacaatga tagggaatgc accaaaagca
    73441 attgttaacg caggaagtac actattacaa aatatcgatt ttacagcatt aatagattca
    73501 ttacttccag tagtaaactt agtaaataat ttactagata gtataaacaa taaatcacca
    73561 attgcacaag gattaataag tatatttggt acaatagtaa ctacagcatt ccaactaatc
    73621 ggacctgtag ttgaagctgt tagtcctatt atcacaagaa tttttacttt tttaggtgaa
    73681 tatgcacctc aaataaacaa ttttatagag acactgggtg ttatttggaa aactgtatgg
    73741 gagaccttag gacctctgtt ggaaactgga tggaaaatta tagagccaat attgggagct
    73801 ttttttaaca tattagataa agtatgtaaa atagttaaag atatatgcaa gtggtggcaa
    73861 actatgatta ataagataaa aaatggaagc atcacaggaa cagttttaaa tctagtggaa
    73921 aagagtaaaa aaaattacaa agataatcca tatgctggaa caaaggctgg tgattctggt
    73981 aaagcttatt caggtaagaa aggtaataat gcatttggat tgaactatgt tccttataat
    74041 gactatcaaa ccagactcca tgaaggtgaa atggttttaa ctaaacaaga agcaaatcaa
    74101 tatagaagca gaaaaaatgg tggaaatata aacatagcta agttagctga tacaatagtg
    74161 attagagaag aagctgatat agaaaagata acatcaaaat tagttgcaag tatccaattg
    74221 gcacagttag ggggtgtctt ataatggaaa tgtggcttag acaagctgaa gatagattta
    74281 gatttccagt atttccatct tcctttagta ttaatggaaa agctgctgta aactcttcta
    74341 gtatactcaa aataggtgaa atagcaactt ttggtggtgt agctcttaaa agcatttcaa
    74401 tatcaagttt ttttccaaat aaagactata ctttctgtga ctatacaggt tttccatcac
    74461 catatgattg tgtaaataag atagaaaaat ggatgaagga aggttttata ttaagattta
    74521 caattacgga aacaaatata aatatggaag tcataattga agggtttagt tatgaagaaa
    74581 gagatgggac tcgagatgta tattttacat tagatttaaa agagtataaa agaataaaga
    74641 taccaaaagt aactccaaaa caataactat tatagataat aagttataaa taactgctta
    74701 tagaattaaa tgaaaaggca ggtgattttt tattattaag atttgggtac acataaaaaa
    74761 cggaagtata tatgacataa ctgacatagt agacaaggta tcatggtcag gtgattataa
    74821 atctccatca aggacactag agttttcaat aatacaatca tcatttgatg taaatttcca
    74881 acaaatagat ataccaatag ctagtacagt ctgtttctat gtagatgaga aagaactctt
    74941 tagaggaatg ataattaata ggtctaaaga ttcaagcagt aatgaaatta gttttgtatc
    75001 taaagatatg ggatttttac ttacacaaag tgaagtgtca tacaatttta aagataagtt
    75061 agttgaagac atagcaaagc aagtatttgc tgaaaatagg ctttcagttg gaacaatagc
    75121 aaagaccaat gtcaagtata caaagatgtt tataggagta aatggttatg acacaataat
    75181 gagtgcatat acagaagcaa gtaaaaagac aaagaaaaag tatatgatag aggccaattt
    75241 agataagttt aatgttattg aaaaaggaac tgttacatta aatgttatgt ttgaagaggg
    75301 atttaatatt ataaatacca ccttttcgga gagcatggaa aatgtaaaaa ataaagtaat
    75361 agtggtagac cagtatggaa gcaagattag cgaaaaaata gataatgaaa tttttaagga
    75421 agtaaatgta ataatgcaaa aagtaattca gcaacaagaa aatcaagatg tagatattga
    75481 tagcgagttt aatgggatag aaaaaagctg ttctcttaaa ggttatggag atgtaagttg
    75541 tataactggt agaggagtaa aagttaaaga ttcttataca aagcttgtag gactatttta
    75601 tatagataca gacaaacata cttggcaaaa tggagaatat caaattgagc ttgaacttaa
    75661 ttttcaaaat cttatggatg aaaagtcagc aggacaggat gaacctaagg aagaaagtaa
    75721 cttaggggga gaagattatg caggaggaaa agagtttaca gcagaattta cagcttactg
    75781 tcctagaaaa gaagaaggtg gagatacaga ttgtagaaag aaaaaacttg acccatctaa
    75841 aaaaacttgc gctgctccta tggttggtaa atatgaacaa acttattata caaaagagtt
    75901 tttaaataaa catcctttat taaactatgg agatgaaata caggtaatta caggagtttc
    75961 tggtcgtgat ggagtctata aagtaaatga cgtaggacct gcaataacta tagaaaagaa
    76021 tggaacatac catatagata ttttatttgg aaatgttgaa gaagctagta aatttggaag
    76081 aagaaaagga aaaattatta ttggtggtta ttctggtaat gtatctgata aagctaaaat
    76141 agtaatatca gaagcaaaaa aacatctagg taaaccttat aaatggggtg gaaatggacc
    76201 aagtagtttt gactgttctg gtttaatggt ctattgtttt aaaaaagtta atgttagttt
    76261 gccaagaacg tcaaatcaac aatctaaaaa aggcaagaaa gtagaacaaa aaaatcttca
    76321 agcaggagat ttagtatttt ttcataatcc agtcagccat gttggattat atataggtaa
    76381 tggagaattt ttacatgctc cacaaaaagg tgatgtagtt aaaataagta agttaagtag
    76441 tagaagagat tttaatacag ctaggagagt attataaaag gatggtgata taatggctaa
    76501 tccaataaat gaatttatag gaataataag agaagaagga aagtatcata atcaaccttc
    76561 tttttttatt ggaaaaatta aaagtaaatt accagattta aaaatagaga caaataacat
    76621 catattagaa aaagaagata ttttgataga tagttggatg attgatagac agctagaaac
    76681 atttgacaca gaaacaaatc aagaacacca gcatgaagta aaaaatcctt ttatagataa
    76741 ttttgaatct ggggatatgg taataatgtt tagaataggc gaaaaatttg ctgttgtaag
    76801 taagttggtg agcttataat gagtacaata tttcctttta taggtgtccc agaggattat
    76861 atcttaccta aaacagaaga attgccaatc tttcgtgaag tggcatggga ttttgaaaaa
    76921 gatgaaccta ttttagaaaa aggtgacttt aaaataattg aaaaaaaaga agccttaaaa
    76981 gtttggatat acaagtgtat aaagacaaat agatatgaac atgagatata ctctttagaa
    77041 tatgggacag agctttcaga actaatagga caaaaatata caaaaggtct tacagaaagt
    77101 gaagctagta gattcataaa agaggccctt ctaataaatc catatatatt agaagtaaac
    77161 gtaaaaagtg ctaactttaa cagagacgta ttgagtgcaa atgtaaaagt atccactatc
    77221 tatggggagg tggaaataaa tgtatagtga ccagacatat gaagtaataa aaaatagaac
    77281 tcttgaaaat attaatcttg atatttataa aggagaaggt tcttttctaa acaacatggt
    77341 atctggaaat aatctagaac tttcaaagat atatctagaa ctttcaaaga tacataaaat
    77401 ggcttttata caagacacat ataaccagtt tctcgataaa agagtcaatg aatttggtgt
    77461 atatagaaag ttaggtacag agtcaaatgg agaagttgaa tttattggag agaaaggaac
    77521 tgtaataaat aatggcacaa taatatcata tagagattta ctatttgtag taataaaaga
    77581 tgtaactatt ggtagtgaag aaggtgacaa tagcccagtt caagctctgg aagttggtaa
    77641 gaaatataat ttacctacaa attgtgaatt taaactagtt gataatatat ctggagtaac
    77701 aaagattact aacacaagaa gttttgaagg tggtacagat atagagacag atgaagaact
    77761 aaaagaaaga ttttataaaa tccaaagaaa tcaagctaca agtggaaata aagctcacta
    77821 tgaagaatgg gctttggaag tagatggagt ctataatgtt aaggtttatc caagatggga
    77881 tggtccggga acagttaagg tcttgatatt tgggaaaaat aatcaagctg ttgatacaga
    77941 aacaattgaa aggtgtcagc aacatataga tgaagagaag cctattggac caactataac
    78001 agttgtgaca ccattaccaa tagaaataaa tataagtgca gtaatgaaat tagaagatgg
    78061 atatacatta gacaatgtaa aagaatcttt cctagaaagt ataaatacat actttagaga
    78121 tattagagga gagataatct atacaaaagt aatgggaata cttataaata ctactggtgt
    78181 acacgattta agtaacctac ttataaatgg aagtacagat aatataacta ttaatgaaga
    78241 taaaatacct agtgtaacaa ctgttaattt tagtgaggtg gaaaatcaat gaagctaatt
    78301 gataaactac catcatttga tagaaattac attgtagagg agatacaagg tgcatacgat
    78361 acagaattaa atattcttaa agaagatatt gatgatacct ttaaccaatt atttgttgac
    78421 actgcaacat ggggattaga tatgtgggaa gacatactct gcattgaaaa aaaagaactt
    78481 gattttgaca caagacgtag caatataaaa gctaaaatga gaagcagagg tactagtact
    78541 attgaagtta taaaaagtat atgtgaggca tatacaaaat cagaaacaga tataaaagtt
    78601 tatagtgatg aatttacatt cgtattgagt tttatagcaa ataattgtga ctataaaact
    78661 cttttagatt gtagcgatat gattgaaaga gtaaaacctg ctcacttatt acactattta
    78721 gaaccaataa tactagataa aagtatggtc tattgtggtg gaggtatggt atgtagtgaa
    78781 gaagtaaaag ttcatccata ctttgaacca attataaaat gtagcgctgt tgtaaactgt
    78841 ggagctggaa tgttaagtag agaagaaata aaggtttatc ctttaagcat taaatgcatt
    78901 gaaaataatt gtaagattaa tatagctatt gcaaatgata caggcgtaga aaatgtagta
    78961 gtttatccta aatcggaggt ggtataattg gaagaaaaat tttatataat attaaccaaa
    79021 attggtagag aaaaaatagc aaatgcaact gcactaggag agcttgttgg attaaccaag
    79081 tttcaagttg gagatagtaa tggagaatat tatgagccaa cagaggaaca aactgcttta
    79141 aagaatgtag tttgggaagg aaatataaat tctctaagaa ttgatgaaaa aaatcctaat
    79201 tggatagtta tagagactat tttaccagga acagttggtg gatttatgat aagagaagct
    79261 gctgttctgg ataatgagaa taatataata gctataggta agtatccaga gacgtataag
    79321 ccacgtgctg aagatggcag tattaaagat ttggttgtaa aaatgatttt acaattgtcc
    79381 aatacttcaa atgttacatt agaagtagac ccgacgttgg tttttgtaac tcaaaaggat
    79441 attcaagatt tagatgataa ggttgataaa aatataaaag aaataaaagt aaaaattgga
    79501 gaagaactct tatctacaga agctaaaaac ttatcaggag ctataaatga ggtagtagaa
    79561 aaaattaaaa atatatctat tgatgatgta ataggaggtc aaatacaaac tgaactatct
    79621 gtattaaaaa atagttacaa taaattatct gaaaaagtat tagatatttt aatatactta
    79681 gaattagagt cagaaataga tgtagatgaa gctggatatt ggtatgatac cttaactaat
    79741 gctaaaaaca taatagctat agaaggcctt aagttagatt taaatagaaa gtgtataact
    79801 ggagaacttg gtagtgttac atttaagaat gtggttctac catttaatgc aaatagagtt
    79861 agatatatac atgaaatgga taataacttt gttgaaacaa aatctaatag ggcatattca
    79921 attggccaga cagatataac tttaaataaa tattcgtatg aaataagata attaggaggt
    79981 ttttataatg aaaagaacta aactacttca aagaggtaat ttctttggcg ataaaaatat
    80041 ggtagttgat gaatttgatg aagggtatga taattatgac tttattaatt tttttactgg
    80101 atgttgtaac tatacatttg gtctaaaaaa taataatatc ttgtatggat gtggagataa
    80161 tagtaacttt caacttggat tgggagaaga caatacaaca agaaaattat ttacgaaaat
    80221 accaaatata tctactaata ttaaaaaagt tgcatgtgga gaatctcatg cagttatact
    80281 tacttcagat ggagaattac ttgtcgcagg tataaataca gatggtcaaa tgggattggg
    80341 attagaaaaa gtaggaaaaa cagtttccac atttgagaag gttccagaaa taaaaggcgt
    80401 aaaggatatt gcatgtggac ttcaatcaac atatctttta tacaatgatg gaactttata
    80461 tgttgctgga aataatttgt atggtcaatt aggtctagga actaatggag catctgcaaa
    80521 tgtaaataca tttataaaag tagatgttga taatgtaaag gctgtattct catataataa
    80581 atcagctttt ataataaaga atgacaataa atgttattct actggtttta ataatcaagg
    80641 tcaactaggt ttaggagata agaataatag agatttattt agcttagttt ctattaatga
    80701 tgttaagact atagcttgtg gttctgaaca cactgtgtta atgacgtata ataatgatat
    80761 atatggttgt ggaaaggaaa aatgttttgg aaatgcactt caatcatcac tatttactaa
    80821 gatagaagaa gtaaatataa aaactattgc atgtggtaat ggtaatacta tgcttataga
    80881 taataaaggt attttgaagg ttgctggaaa taatgatata tatcagttag gtatagcaaa
    80941 ttactctgag aatatagata attcatttat agatttaaaa aatattgtag ctaagaatat
    81001 tttcattggt ttatcacata gcatactaat tgattcaaat aatgattcat attgtacagg
    81061 agataatact tatggacaat taggttcgtt ttttgatgat atgcacattg tagaatttaa
    81121 gaaaatggat agtgaaaaat atagttatag taattatata aatttaatta aatctgagga
    81181 taaattaact ttattaaaag aagaaatgga aataaaggat attgaacttc cactagatat
    81241 acattctgta agagatgtcg tttttagtcc ttattgtact ctggttattt tagggaatgg
    81301 agatgtatat ggtctaggaa ataatagata caaaggaatg ggttctgact taccaagtca
    81361 attaaatgag ttgacaaaat taagtatctc taatgtaaag tctatagtag catcaaaaaa
    81421 tatttctgga ggaatattct acattaaaaa tgatgacact tgttattatt ctgggccaaa
    81481 tagtaactca atagcaggtg ttcttccttc taattcagat atatttaaga aaatatctat
    81541 agataatgta aaaaaagttg ttataaatac tgatttatca aactggtttt cattaattgt
    81601 aactaataat aagcaaatat atacttctgg aaagagttca agttatgtta atggacttag
    81661 taataaatca gcaagtcaat atactgagat tggtgttagc aatgtaacag atgcatatag
    81721 ttcatataat gcaacattta ttgtggctga cgaaaaaaag gtatatgcaa ctggtataaa
    81781 tacaaactat ttattgggtg ttaatcctaa tgatggagtc aatataaatt taacattaat
    81841 aaataactgg ttttatgtaa atccttcagg agcaagttat agtacaaatt cttgtacaaa
    81901 tgatattact aaaattaata atattattat atatgaatat atggctatat tatgtacaaa
    81961 ttatggttct tttttaactg gataccatgg tactttatgg acaaagccaa gtgattctac
    82021 ttatagagtt caacaacaag gaattacaca tgctggatat attagtaatt acatatataa
    82081 ctattatccc acaaggtgta cacaatcatc atattctaca aattttgcct atttgtatta
    82141 tggagaatca acaagtaatt taaaaaatgt aaatccagat aatttactta tttctggagg
    82201 ttcatcttat atacatcaat atggaaggaa ttatcttaac aatcaatcat ctaataatat
    82261 tgcagcatct aatataaatt caggtcctat tacctctgat aaagccatat ttttatataa
    82321 agctctattg tatttatctt ctaacacgtt atatggtttt gggaatatat ctgaaagtgc
    82381 aaaagaacta gatgtttcag atacacaaga tggatataat gccactaatt ataaaaaggt
    82441 aatgaaaaat ataaaaaata tatttatacc tccttatgat ttaagtagag ataaaactag
    82501 atttgcaata ttaactgata agagcttatt tatatgtgga tataactcta agggtacgca
    82561 tggtatatca gttaatagta gtttaaattt aaataataag ataaattaca ataaaaagaa
    82621 tagcagtagt gaaatatctt ctaatataca agaaatatat agccattcga agtctacata
    82681 tttattaact aataataata tgctttacag tgttggttta aatgatgtag gtcaattagg
    82741 agtgggagat gagataaata gaaaggtatt tactaaaata aatattgata atataaaatc
    82801 tataaatgta aatagattta ctgacaatag taaacatgta tttgcgataa aaaatgataa
    82861 tacctgttat gctgttggtt taaataattc tggtcagtta ggaataggag ataatgtaaa
    82921 tagaaatgta tttactaaaa taaatgttga aaatgtaaaa tatgtagctg tatatggaaa
    82981 cacatctcta ttattaacta atgatggtct tttatatgga gcaggtaata atggaaaagg
    83041 acagttagga ttgggtgata ctacaagtag gaatatattt acacgtatac ctataaatgg
    83101 agttagagat gtgtatatat gtaatgatgt atcaataatt gttaaaaatg ataatacatg
    83161 ttatgtatgt ggatttgtaa atggatattt tgggtttact gaaggaagta taagtacatt
    83221 tacaaaaata aatattgaga atgtaaaatc tgttgtaaca gcaggaagtg aagctacatt
    83281 ttttataaca aatgacaatg ttatttatac tacagggaaa aaagagaggg tattcttttc
    83341 aacagaaact aatgatataa agggtatacg agtaattaat aatattataa atgcaaaaaa
    83401 aatagtagtt aatggatata cttcagccat tttaacaaat gacaataaac tatttgttgg
    83461 aggtcttagt ggatatggaa gtatagcaaa taataataat acaaatagtg tggaagatgt
    83521 taaagatgtt tttgtaacag ctaataatac actttatata gataataata acaatttgat
    83581 atcatcaggt agagatactt atggtatatc tgatgaatct tatagggata tgtcagttcc
    83641 atattataaa gtatctataa agaaagatgt tgatactgta ttttctagtt acaatactat
    83701 atttattaaa gatatatatg gaaaatttta ttcttcaaca ggagataata gatataatca
    83761 tttaggtatt caccatagat atgataatga taaaaatgaa gctcttgaag gttccctaca
    83821 ttcatatttt aaaacagata acacatcaga taaaatagtt tttaataaga aaaatgaaaa
    83881 gctagtaatg tttaatgata agtatataaa aacaaataat aagtatataa attataaaaa
    83941 catatttaaa gataatttta agtatacttc aataatattg ccatttgagg tatctgatat
    84001 tgatatatca aaaacacatt cattggctgt tgctaaggat ggcaagttat atggaatagg
    84061 aagtaattca tataaagaaa ttaatcaaac ccttgaagat atagaattat taactcttac
    84121 tgaagtaaat atatcagatg tcaaaaaagt tgcttgtgga gataactact cctatattat
    84181 taagacagat aatactctat ggtcatatgg aaagaatact gagtaccaat tgggagttgg
    84241 ccacaataat gatgtaagag agttacaaaa ggttactgga ttaccttctg ttaaagatat
    84301 aagtatatat aactcaatga cacttgtttt aactaatgag ggagagttgt acgctcaagg
    84361 gtacaataca aatggattat ttggactagg agaaagtgaa aaagataaga taataagaac
    84421 ttttactaaa gtattaacta atgttaaaga aattaagtca cataatgatg accacatact
    84481 agtaattaaa aatgataata gtctatggat aactggtaaa aataaatcta tgtataaaat
    84541 atctatatca attactgatt tatatgaatt tactaaaata ccaattcctg aacatctaaa
    84601 tgatatttta gatatagagc tttcagatga tacaatatac atgataacaa aagtagatac
    84661 aagtaaagca tctatagaaa tagttgaaaa atcaatatct caagtgagag ttgtagtaca
    84721 agaccctaat aatgttatag aaaaacttga aatgtttata aatgatgaat taatatctac
    84781 taagactaat ttggaaataa atagcattat atttgagata ccacaaaata aaatagtatt
    84841 aggagaaaat aagatactga ttaaagccag tagtcctaca ggcgatttat attcaagtat
    84901 gtttatattt aaatcagaaa cagggcttaa agtaaaaaag gattctattt taatgataaa
    84961 caataaagta tattcaatca taaacattac tgaaaataac actgatttaa tagtaacatt
    85021 aaatgaggga ttaaaggatg acatgatgga aaacaatcct atatatcaat taataaataa
    85081 aactaaagtt caagtaaaaa taaataaatc tgacttattc aaagacatga aactagttga
    85141 aatcaaaaaa tcagactcaa gttaccaaga aatctatgaa ttagaagaag ccaacataaa
    85201 aagtgctcag cctaaaatca tagtagaaaa aggagataaa tggacagcta taaaacgtcc
    85261 atctatgatt tttagatatg atgctgaaag caacgagcca caagcttaaa atggaggtgt
    85321 gaaaattgtt taaattcgat aaaaataaaa tagaacaaat caaacaaggt agaaaagtag
    85381 aaatgcagta taaagacatt tcagacataa gtataggtca agtaaagcaa gatgatgata
    85441 taacaaataa ttttatagca aatgcagaaa tatatgagat gttgttaaat caaagttctg
    85501 tcaatgaagc aagtaatata agctctttta gtgtaagaaa atctggaggt gagagtggaa
    85561 tggtagaagt atatgtagct ttaattttaa gaggcaaaaa aacaatagaa gaagtaccag
    85621 cagtaattag agagcaagtt agaattagat gcaaagaatt agaaatacca gttgaatagt
    85681 aaatttagaa taactatgta ttagttattt tttttatgta aagtacaagg tcttaacttt
    85741 aataagtaag ccttgtactt attttttgtt atattagaaa ttgtatatat atttattatt
    85801 tattcaatct ataaattgca cctacaattt aaagtacaga agattaaatt gataatcctg
    85861 aaaatataat attgcatgat gtaagaatat aacaaaaatt aaagctataa gtataaaaaa
    85921 tttagacaat aggaggctat aatggataaa ttaataaccg aattgagtag tctaggggca
    85981 ataggtatac tatgtgctct attatttaaa aatactatgc aggagaaaaa agaagataga
    86041 gacatgtata aaaaaactgt agaaaatttt atagaattat ctacacaaca acaagaaata
    86101 aacaaaaata tacttgttca aatgggtata atgaaaacag atgtagagga aattaaggaa
    86161 gatgttactg gtataaaagg tatgttacaa aacggtgtat aacatgaaag agtagcacca
    86221 gattatatat tgttaggaaa agataaagta gtattgtaga tagtacccta ttttattgag
    86281 aaggatttga tatttaaaaa tattaattaa aaaaagtaat aaaaataata tataaaaata
    86341 atatataaaa attaaaaaag gagttgagct taaatttgat tagaaaaaat caattttaag
    86401 acaactcctt ttttttatta aattattgtc tattaagcaa aatagctatt ttagcatctg
    86461 gattataact tatttgaacc atttgatttt tcttaacatg ttcaaggtct tcaccaccat
    86521 aagctatttg taacttaact ggtaacttac cttgttttat aatagcaacg tactcttttt
    86581 tacctttttt ctctaaacta atcaaattgc caacataagg tttaaagttc tgatactttt
    86641 tactagaatt tcttatgtag aagaaagcac caacagcaat aactaaattt atgccaagtg
    86701 taacccaaga attgatttta agcatagctc cagcgattat tatcacgaac attaaaacga
    86761 taggtaatat agctttctta agaagcaatt tacccattat ttcattagcc tttttctcag
    86821 ggccactcat agtttttgat ctagcaaatg attgcgcgaa tttgtctctt aagcccattt
    86881 tatcctccta attttaataa atatttagtt ataataacga gatattactt gaaactaaaa
    86941 atttactaca tttatattat gtttgacttt tgtataaata attacattc
//
LOCUS       DAYAOY010000003        78514 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYAOY010000003 DAYAOY010000000
VERSION     DAYAOY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 78514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.003"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            303..1583
                     /locus_tag="ACGI6B_000228"
     CDS             303..1583
                     /locus_tag="ACGI6B_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFG9749703.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1846..3237)
                     /locus_tag="ACGI6B_000229"
     CDS             complement(1846..3237)
                     /locus_tag="ACGI6B_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFG9749704.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(3248..4468)
                     /locus_tag="ACGI6B_000230"
     CDS             complement(3248..4468)
                     /locus_tag="ACGI6B_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFG9749705.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4482..5270)
                     /locus_tag="ACGI6B_000231"
     CDS             complement(4482..5270)
                     /locus_tag="ACGI6B_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFG9749706.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5287..6561)
                     /gene="larA"
                     /locus_tag="ACGI6B_000232"
     CDS             complement(5287..6561)
                     /gene="larA"
                     /locus_tag="ACGI6B_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HFG9749707.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6679..7392)
                     /locus_tag="ACGI6B_000233"
     CDS             complement(6679..7392)
                     /locus_tag="ACGI6B_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFG9749708.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7517..8776
                     /locus_tag="ACGI6B_000234"
     CDS             7517..8776
                     /locus_tag="ACGI6B_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HFG9749709.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            9056..9622
                     /locus_tag="ACGI6B_000235"
     CDS             9056..9622
                     /locus_tag="ACGI6B_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9749710.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9823..11028)
                     /locus_tag="ACGI6B_000236"
     CDS             complement(9823..11028)
                     /locus_tag="ACGI6B_000236"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFG9749711.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(11131..11237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11329..11697)
                     /locus_tag="ACGI6B_000237"
     CDS             complement(11329..11697)
                     /locus_tag="ACGI6B_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HFG9749712.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11749..13026)
                     /gene="obgE"
                     /locus_tag="ACGI6B_000238"
     CDS             complement(11749..13026)
                     /gene="obgE"
                     /locus_tag="ACGI6B_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFG9749713.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(13137..13427)
                     /gene="rpmA"
                     /locus_tag="ACGI6B_000239"
     CDS             complement(13137..13427)
                     /gene="rpmA"
                     /locus_tag="ACGI6B_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFG9749714.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13441..13758)
                     /locus_tag="ACGI6B_000240"
     CDS             complement(13441..13758)
                     /locus_tag="ACGI6B_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFG9749715.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13773..14084)
                     /gene="rplU"
                     /locus_tag="ACGI6B_000241"
     CDS             complement(13773..14084)
                     /gene="rplU"
                     /locus_tag="ACGI6B_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFG9749716.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(14185..15606)
                     /locus_tag="ACGI6B_000242"
     CDS             complement(14185..15606)
                     /locus_tag="ACGI6B_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFG9749717.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15624..16325)
                     /locus_tag="ACGI6B_000243"
     CDS             complement(15624..16325)
                     /locus_tag="ACGI6B_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HFG9749718.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16327..18171)
                     /locus_tag="ACGI6B_000244"
     CDS             complement(16327..18171)
                     /locus_tag="ACGI6B_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HFG9749719.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18490..19683
                     /locus_tag="ACGI6B_000245"
     CDS             18490..19683
                     /locus_tag="ACGI6B_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HFG9749720.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19895..20715)
                     /locus_tag="ACGI6B_000246"
                     /pseudo
     CDS             complement(19895..20715)
                     /locus_tag="ACGI6B_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(20845..21636)
                     /locus_tag="ACGI6B_000247"
     CDS             complement(20845..21636)
                     /locus_tag="ACGI6B_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFG9749721.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(21640..21969)
                     /locus_tag="ACGI6B_000248"
     CDS             complement(21640..21969)
                     /locus_tag="ACGI6B_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9749722.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22454..22867)
                     /locus_tag="ACGI6B_000249"
     CDS             complement(22454..22867)
                     /locus_tag="ACGI6B_000249"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFG9749723.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(22908..24038)
                     /gene="rodA"
                     /locus_tag="ACGI6B_000250"
     CDS             complement(22908..24038)
                     /gene="rodA"
                     /locus_tag="ACGI6B_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HFG9749724.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(24123..24407)
                     /gene="minE"
                     /locus_tag="ACGI6B_000251"
     CDS             complement(24123..24407)
                     /gene="minE"
                     /locus_tag="ACGI6B_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFG9749725.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24425..25222)
                     /gene="minD"
                     /locus_tag="ACGI6B_000252"
     CDS             complement(24425..25222)
                     /gene="minD"
                     /locus_tag="ACGI6B_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFG9749726.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(25246..25929)
                     /gene="minC"
                     /locus_tag="ACGI6B_000253"
     CDS             complement(25246..25929)
                     /gene="minC"
                     /locus_tag="ACGI6B_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFG9749727.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(26027..29005)
                     /locus_tag="ACGI6B_000254"
     CDS             complement(26027..29005)
                     /locus_tag="ACGI6B_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFG9749728.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(29017..29505)
                     /gene="mreD"
                     /locus_tag="ACGI6B_000255"
     CDS             complement(29017..29505)
                     /gene="mreD"
                     /locus_tag="ACGI6B_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFG9749729.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29520..30413)
                     /gene="mreC"
                     /locus_tag="ACGI6B_000256"
     CDS             complement(29520..30413)
                     /gene="mreC"
                     /locus_tag="ACGI6B_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFG9749730.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30423..31484)
                     /locus_tag="ACGI6B_000257"
     CDS             complement(30423..31484)
                     /locus_tag="ACGI6B_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFG9749731.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            complement(31554..32213)
                     /gene="radC"
                     /locus_tag="ACGI6B_000258"
     CDS             complement(31554..32213)
                     /gene="radC"
                     /locus_tag="ACGI6B_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFG9749732.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(32258..32842)
                     /locus_tag="ACGI6B_000259"
     CDS             complement(32258..32842)
                     /locus_tag="ACGI6B_000259"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFG9749733.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(33062..34033)
                     /locus_tag="ACGI6B_000260"
     CDS             complement(33062..34033)
                     /locus_tag="ACGI6B_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFG9749734.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(34049..34624)
                     /gene="rsxA"
                     /locus_tag="ACGI6B_000261"
     CDS             complement(34049..34624)
                     /gene="rsxA"
                     /locus_tag="ACGI6B_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFG9749735.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(34639..35232)
                     /locus_tag="ACGI6B_000262"
     CDS             complement(34639..35232)
                     /locus_tag="ACGI6B_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HFG9749736.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(35245..35814)
                     /locus_tag="ACGI6B_000263"
     CDS             complement(35245..35814)
                     /locus_tag="ACGI6B_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HFG9749737.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(35814..36791)
                     /locus_tag="ACGI6B_000264"
     CDS             complement(35814..36791)
                     /locus_tag="ACGI6B_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HFG9749738.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(36814..38151)
                     /gene="rsxC"
                     /locus_tag="ACGI6B_000265"
     CDS             complement(36814..38151)
                     /gene="rsxC"
                     /locus_tag="ACGI6B_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFG9749739.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38367..39311)
                     /locus_tag="ACGI6B_000266"
     CDS             complement(38367..39311)
                     /locus_tag="ACGI6B_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749740.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39422..40696)
                     /locus_tag="ACGI6B_000267"
     CDS             complement(39422..40696)
                     /locus_tag="ACGI6B_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9749741.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(40828..41199)
                     /locus_tag="ACGI6B_000268"
     CDS             complement(40828..41199)
                     /locus_tag="ACGI6B_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9749742.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41427..42026)
                     /locus_tag="ACGI6B_000269"
     CDS             complement(41427..42026)
                     /locus_tag="ACGI6B_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749743.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(42227..42439)
                     /locus_tag="ACGI6B_000270"
     CDS             complement(42227..42439)
                     /locus_tag="ACGI6B_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFG9749744.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(42512..44227)
                     /locus_tag="ACGI6B_000271"
     CDS             complement(42512..44227)
                     /locus_tag="ACGI6B_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9749745.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(44227..44781)
                     /locus_tag="ACGI6B_000272"
     CDS             complement(44227..44781)
                     /locus_tag="ACGI6B_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFG9749746.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(44774..45376)
                     /gene="coaE"
                     /locus_tag="ACGI6B_000273"
     CDS             complement(44774..45376)
                     /gene="coaE"
                     /locus_tag="ACGI6B_000273"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFG9749747.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45385..48033)
                     /gene="polA"
                     /locus_tag="ACGI6B_000274"
     CDS             complement(45385..48033)
                     /gene="polA"
                     /locus_tag="ACGI6B_000274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFG9749748.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            48185..48272
                     /locus_tag="ACGI6B_000275"
     tRNA            48185..48272
                     /locus_tag="ACGI6B_000275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48219..48221,aa:Leu,seq:caa)
     gene            complement(48459..48902)
                     /locus_tag="ACGI6B_000276"
     CDS             complement(48459..48902)
                     /locus_tag="ACGI6B_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749749.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(48905..49102)
                     /locus_tag="ACGI6B_000277"
     CDS             complement(48905..49102)
                     /locus_tag="ACGI6B_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749750.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(49294..50160)
                     /locus_tag="ACGI6B_000278"
     CDS             complement(49294..50160)
                     /locus_tag="ACGI6B_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9749751.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(50386..51255)
                     /locus_tag="ACGI6B_000279"
     CDS             complement(50386..51255)
                     /locus_tag="ACGI6B_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9749752.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(51761..51982)
                     /locus_tag="ACGI6B_000280"
     CDS             complement(51761..51982)
                     /locus_tag="ACGI6B_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749753.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(52483..53463)
                     /locus_tag="ACGI6B_000281"
     CDS             complement(52483..53463)
                     /locus_tag="ACGI6B_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749754.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(53627..54499)
                     /locus_tag="ACGI6B_000282"
     CDS             complement(53627..54499)
                     /locus_tag="ACGI6B_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9749755.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(54663..55214)
                     /locus_tag="ACGI6B_000283"
     CDS             complement(54663..55214)
                     /locus_tag="ACGI6B_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9749756.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(55234..56136)
                     /locus_tag="ACGI6B_000284"
     CDS             complement(55234..56136)
                     /locus_tag="ACGI6B_000284"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFG9749757.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(56151..58523)
                     /locus_tag="ACGI6B_000285"
     CDS             complement(56151..58523)
                     /locus_tag="ACGI6B_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HFG9749758.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(58744..59433)
                     /locus_tag="ACGI6B_000286"
     CDS             complement(58744..59433)
                     /locus_tag="ACGI6B_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9749759.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(59674..60795)
                     /locus_tag="ACGI6B_000287"
     CDS             complement(59674..60795)
                     /locus_tag="ACGI6B_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9749760.1"
                     /translation="MGYIKYSIFFYLFSVYYKCLDAVIYRILFFAITGNVISSKESLL
                     VPYLESIKIQIYSNLIIILIYLIFSFFRKSIKLISQDKRNYVYLLFALLVNTMNMLVN
                     YVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNK
                     LDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKY
                     IYNTGNMILDIIINEKSEVCLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEA
                     CDKIVDENINKYIRIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQ
                     SIKSSLQKYNGELLFENSIDKFILNIYIPLDQDTDSWGD"
     gene            complement(61047..61760)
                     /gene="rgbR"
                     /locus_tag="ACGI6B_000288"
     CDS             complement(61047..61760)
                     /gene="rgbR"
                     /locus_tag="ACGI6B_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HFG9749761.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(62010..62561)
                     /locus_tag="ACGI6B_000289"
     CDS             complement(62010..62561)
                     /locus_tag="ACGI6B_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFG9749762.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(62665..63468)
                     /gene="zupT"
                     /locus_tag="ACGI6B_000290"
     CDS             complement(62665..63468)
                     /gene="zupT"
                     /locus_tag="ACGI6B_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFG9749763.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(63619..64782)
                     /locus_tag="ACGI6B_000291"
     CDS             complement(63619..64782)
                     /locus_tag="ACGI6B_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9749764.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(64842..66029)
                     /locus_tag="ACGI6B_000292"
     CDS             complement(64842..66029)
                     /locus_tag="ACGI6B_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749765.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(66392..68092)
                     /locus_tag="ACGI6B_000293"
     CDS             complement(66392..68092)
                     /locus_tag="ACGI6B_000293"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9749766.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(68335..68610)
                     /locus_tag="ACGI6B_000294"
     CDS             complement(68335..68610)
                     /locus_tag="ACGI6B_000294"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9749767.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(68729..69301)
                     /gene="mobB"
                     /locus_tag="ACGI6B_000295"
     CDS             complement(68729..69301)
                     /gene="mobB"
                     /locus_tag="ACGI6B_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFG9749768.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(69285..70466)
                     /locus_tag="ACGI6B_000296"
     CDS             complement(69285..70466)
                     /locus_tag="ACGI6B_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFG9749769.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(70677..71411)
                     /locus_tag="ACGI6B_000297"
     CDS             complement(70677..71411)
                     /locus_tag="ACGI6B_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749770.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            71773..72678
                     /locus_tag="ACGI6B_000298"
     CDS             71773..72678
                     /locus_tag="ACGI6B_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HFG9749771.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(72838..73680)
                     /locus_tag="ACGI6B_000299"
     CDS             complement(72838..73680)
                     /locus_tag="ACGI6B_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9749772.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(73664..74422)
                     /locus_tag="ACGI6B_000300"
     CDS             complement(73664..74422)
                     /locus_tag="ACGI6B_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9749773.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(74439..74909)
                     /locus_tag="ACGI6B_000301"
     CDS             complement(74439..74909)
                     /locus_tag="ACGI6B_000301"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9749774.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(74909..75652)
                     /locus_tag="ACGI6B_000302"
     CDS             complement(74909..75652)
                     /locus_tag="ACGI6B_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HFG9749775.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(75656..76069)
                     /locus_tag="ACGI6B_000303"
     CDS             complement(75656..76069)
                     /locus_tag="ACGI6B_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9749776.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(76095..76670)
                     /locus_tag="ACGI6B_000304"
     CDS             complement(76095..76670)
                     /locus_tag="ACGI6B_000304"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9749777.1"
                     /translation="MRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSCH
                     RINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRID
                     YMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKVR
                     QSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(76864..77247)
                     /locus_tag="ACGI6B_000305"
     CDS             complement(76864..77247)
                     /locus_tag="ACGI6B_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9749778.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            complement(77352..77729)
                     /locus_tag="ACGI6B_000306"
     CDS             complement(77352..77729)
                     /locus_tag="ACGI6B_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9749779.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLIDKGYLARDIIKFQSYYKIIIDNEEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKNVSKEKLDSLEEYYKNLKE"
     gene            complement(78266..78415)
                     /locus_tag="ACGI6B_000307"
     CDS             complement(78266..78415)
                     /locus_tag="ACGI6B_000307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749780.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
BASE COUNT        24967 a        14275 c         8294 g        30978 t
ORIGIN      
        1 gtatatactt tttttttata ttatatcata tttctagtat ggtaaattgt gtatttcact
       61 aataatatat taacaagtta acttaatgta gattattctt tcccatttgt tataataata
      121 taatataaag agctaataaa gttattttaa taacatttaa ccagtttttt atgtaggtaa
      181 catttctaca tttttataaa tttttttacg ttttttaatt ttataattaa aaatttaaat
      241 tttatacata ttataataaa ttgtgatata tataaaataa ttaaagataa aaggagtatt
      301 ttatgaacat acttggatta atagttgaat ataacccatt tcataatgga cacatatatc
      361 atttatctaa atctatagaa aaaacaaatg caacacatac tgtagcaata atgagtggaa
      421 attttctaca aagaggcgaa ccagctttat ttgataaata cactagagca aaaattgctg
      481 tcgaaaatgg tgtagactta gttatagaat taccaacttt atttgcttgt caaagtgctg
      541 aaattttttc tcatggtgct ataacaattt taaattcact aaactgcata aattctatat
      601 gttttggtag tgaagaagga aatacagaaa ttctttataa tctagctgaa attttagcaa
      661 atgaaccaaa tgaatttaag ttacttttaa aaaaatattt agatgatggt ctgttatttc
      721 caacagctag agctaatgca ctttttgatt atatacatca agacaaaaaa aataatttta
      781 tacaaaatta ttcaaaggaa caattatcaa aaacattaaa ttcatcaaac aacatactgg
      841 gtatagaata cataaaaaac ctcatccatt taaacagtag tataaaacca tttactatct
      901 ccagggtcca atcagaatat aattccgaag aaattgagag tgatatttgt tctgctactg
      961 ctataagaac ttcacttaaa gaaaatacag acctttttca tttaaaaaat gttattccag
     1021 aaaaaactta taacatccta aatgaaaaaa tagataatgg ttttttacct atgtttgatg
     1081 acttcttctt tgatatttta aaaacaatta tacttagaga tatggacact ttaaaagagt
     1141 attttgaagt aaatgaaggc atagaaaaca gaatctataa agatatcttt acatctgaat
     1201 ttctacatga actacaatta gcaattaaat ccaagcgata cactttaacc aaaatcaaac
     1261 gaactctaaa caatatattg ttaggtatta aaaaaagcga cgtgcaactt ataaaaaaca
     1321 tagatactat accttatata agaatacttg cttttaatga taaaggtaga gaaattctaa
     1381 aagagataaa aaataaatct gaaataaaaa taataaacaa gttttcaaat atatcatttt
     1441 cattagatga tacgattttt aaaacactca taagttatga tataaaagct acaaatatat
     1501 ataattcaat atattataaa aacaataaag atttagtaaa aggccctatg gatttttata
     1561 ctcatccaat atatgtaaag tagtttttta aaataactat atttataaaa atcattttat
     1621 taactcttta ttatataaga tacaatagtc ttactttatt tatgtacaca tgcttttagg
     1681 atgacaacat caacttaaat attatgttac tttctaaatt taagtaaata ttgccatcct
     1741 aaaaatttta caatttagct tcagatttaa aagtaaagtt taaaatatta tatttaaatc
     1801 taatttaata ttttttgttt tataaatatt attaaattaa tatttctaat atattttccc
     1861 tggatttaaa atatttttag ggtcaaaagc taactttata tttttaatta acatcatata
     1921 agcatctgaa tttgattcat gtaagtactc ttttttagca tatcctatgc catgttcacc
     1981 agaaacttga ccactaagtt ctctagattt tttatacata tagtcaaaag tttcttttaa
     2041 tcttattttc catgtattat catccatacc atcttttaaa atatatatgt gtagatttcc
     2101 atctcctgca tgaccaaaac tttttattct tatttttaat ttgtcttgaa gctcgtgagt
     2161 gtatcttata aactctgcta tcttatctct tggaacaacg acatcacatt catccatttg
     2221 tgtcgtagat gcctttatag cttctaaaaa tgcacctctg gctgaccata tagaatcgtt
     2281 tctttcttgt gtatctgata taaatacatc taatgcacca ttttctaaac ataaattagc
     2341 tactttttca tattcttttt ctatatcttc agtgctattt ccatcaaatg taagcaaaag
     2401 ataagcatct gatgtattat ctggaaactt tttacctaaa aattcttctg ctgccaatat
     2461 cacatctctt tccataaatt ctatagccgt tggtattgat tttgatttta ttattttagg
     2521 aacagtttct attgccattg ataaatctgg aaatggtata agcaaactta tacttttctt
     2581 tggtaaagga agcaatttta atatagcttt agtaactatt cctaaagtac cttctgagcc
     2641 cactaataaa tcctttatac tatatccaga actattttta accacctttc cacctacatt
     2701 tataatttct ccatttggta gtacaacttc aagtcctctt acataatccc ttgtaacgcc
     2761 atattttact gctctcatac cacctgcatt agtgtttata ttacctgcaa ttgtagcact
     2821 tttttcacct gggtcaggtg ggtaaaataa atctctatct tgaacatatt gacctattgt
     2881 catcaataaa acacctggtt ctactgtcaa tgttaaattt tcataatcaa cttcaagtat
     2941 tttattcatt ttacatagat taatcattat tccaccattt atggcaacag cagcacctac
     3001 aagaccagta ccttgacctc ttggtgtgac tggtatatta tttttatatg cgtatttcat
     3061 tattttagat acttgttcag tttcaagtac atttactaaa acttcagggt acttctcaat
     3121 ccctccaagt tcatcatgtg aaaaatcttc atttatatca cttccaacta atacattttc
     3181 ttctccacaa gtatctatta aaaaatctat atctttttta tcgattaatt tatacatttc
     3241 tatcccccta tttagctgct gatgccatat atttttgatt attatctttg ttattttcta
     3301 ttttttcaat caatttagga ataatatcat aaatatcacc tattaaagct aaatgtgcta
     3361 catcaagtat aggcgcttta tcatctttat ttattgctac tatatagtca gcaccctgca
     3421 tccctgcaac aaattgaact gctccagata caccacaagt tattatcaat ttaggtttta
     3481 cagttctacc actaagacct atttgttttt tagcatctat ccaacctgct tctattacag
     3541 gtcttgttcc tgccacttga gcgtttaact tatcagctaa tttatatatc atctccatat
     3601 cttcttgttt ttttattgct ctacttgcca caactatcac ttcagcttct tccagaccaa
     3661 cttctttatt tttttctttt acactcagta ccttaatttt agattttagt tctttttttt
     3721 ctactttaca taaagttatc tttcctgtag tattctctat tttttctggt gctgaaaata
     3781 ttttatatct tacagtggca aattgtggtc tattattagg tgtgtttata tgagccatta
     3841 tatttccacc aaatgctggt cttatttggt ctaaatcagt atttgattga atatcaagta
     3901 ttgtacaatc tgctgtaaga cctgttctaa atctagcggc aagtcttgga gctaatgacc
     3961 tacctaatgt agttccacca actaatacta tcgttggttt tattttatct ataaagtttt
     4021 ctattgcttt tgaatatggt tctatcctaa aatctttaaa ttcatcttca tcatatacaa
     4081 atacttcatc caccccataa gaaatcaaat tactgcactt atcttttatg tcttttccta
     4141 caaatacaca atacacaggt tgttttattt ttgatgccat ctcacgagct ttacctataa
     4201 gctcgtacgt tacaggatgt atatttccat tatgatgttc aacatatact gcaattcccc
     4261 tccattcatc tttattaatt tgtactttac tactctctat aaattcaaat gcgccttttg
     4321 gtccactttt gacacacaac ttgcacatct tacagccagc acttatttct actatacctg
     4381 acttttcttc tatagcccca aaaggacata tttctaaaat tctctttaag tcagttattt
     4441 tatcttgatt tacaactatc tttgacattt attactcccc cttacaccaa ctttaattcc
     4501 tctaatttat ttacaagtgc aaaacttagt tcatctgcat ttcctcttac aatattttta
     4561 tctgtatttg gctttggagg aaatattctt acaacttgag taggagaacc attaagtcca
     4621 tagttatttt catctttatc ttcaaagtca tctaagctat atacaggtat ttctctatct
     4681 ttagtattaa gttttaattt aaatgaaggt agtcttggtt gaaaaatgtc tttttctaca
     4741 gttattaaac atggatattc aatctcacat acttgtaaat catttggcat atctatttca
     4801 acaactatac tttcatcttt aacttcaatt aattttgtaa cgttagtaac atgaggtata
     4861 tctaaaaatt cagctacttc aggtcccacc tgtgctgtat caccatcagt agtttgtttt
     4921 ccacaaatta ttaggtctac ctttcccatc ttcttaatac cttgtgatat ggtataggaa
     4981 gtagccaata catctgctcc tccaaatctc ctatcactta ttaatgctcc ttcatcagct
     5041 cccatcataa aactttcttc aattaccttc tttgcctgag gaggacccat gctaagtact
     5101 tttagattcg ctttcttatc ttcctttatt ctaagagctg tttctaaagc atataggtca
     5161 tagggattca tttttgaatc tactccatct ctttttagta ctcctgtagt ttcatcaact
     5221 tctactgaag tcgttccagg aacttgttta atacatgtca aaatttccat aaagtacact
     5281 ccttttttat ttaactataa ctgatactcc atcaggtata actactactt tgctctcctt
     5341 accaactata ctattcgcca tttctaaagc ttctttaaag ttaaatgcat gtttcatatg
     5401 catttttttt atcatttcag gattacactt atctgttacc attatcacag tatatttaga
     5461 taatattctc gctagtatct gaaattccca ttggtctgga atcgtatcta ctctatcgac
     5521 acttctaatt ttatctaaaa gttgttttgg agaatctgca tttgctacat tttcataaaa
     5581 agattgacct ccatgaccat cattacatgc tgaaatcatt attattactc caccgtcttt
     5641 acaagtagct tcagctgctg tcattccttt aacagattga taaatatttt ggtctagtgg
     5701 atatccacca tttgtagtta caacaatatc agattttata gaatttactt ttgataactc
     5761 tgttacaaat ctacatcctt tagtatgtgc tttttcgaca tcaccagcaa aggaagcaat
     5821 tattttttta tctttatcta ttacaacatt caatatgaat gctaattttg caactttagc
     5881 tgcatatacc atatcctcat gaataggatt attattcaat tttccagttc ttgaattatc
     5941 actatctata aagtcactac aatgattgta cattatagtc tttgcagatg cgattcctgg
     6001 aagtacactt tttctgccac ctgaaaaacc tgcaaagaaa tgaggttcta taaaaccttc
     6061 tgctatcaaa agttctgcct catatgctag tttgtttata tataaatctc cacctgaagg
     6121 caatatccct actttacaca tagatttatc atcagtagat atatgcataa ctatatcttc
     6181 attatctact atttcttctc ccattttata aattagctct tcccttgtac ttggcctatg
     6241 aaatccagtt gccactataa tcttcacatc aatttctgga ttaacattcc taatccttct
     6301 taatataatt ggcatagtta ttctacttgg tacaggtctt gtatggtcac ttgtaattat
     6361 gactacattc tttttcccct taaccagttc ttccagagaa ttagagccta taggattatt
     6421 catagattct tcaactatct cttcttgagt cttatgttct atataattat ccgattttga
     6481 ctccaaaaca ccgagaaaac gttcatcagg tatttctaaa atcattcctt gttttgaata
     6541 cggtactttt aactttacca tttttaatct cccattataa taaactataa aattggtatg
     6601 tggtcatacc agttttacat ttatatgata gtaccattat ctaccatttg caagaataat
     6661 ttattttata acaaactttt aattttcatt ttttctataa tttttactta taaaatccat
     6721 atgttccttt gctttctcac atgcaagttc tacatcattt tctacaactg cattgtatat
     6781 agcttcatgc tgtttaagca atatattttc attaaaaggt tctgcgatta ttttttctct
     6841 tgcatcttct ataaatccat caaataattt tgatgctgtc ataaaaagac tttgaattaa
     6901 ataattttta cttaatgttg ctagtttact gtgaaatttt tggtctaata acaccagtac
     6961 tcgattatta tcttttttat tagtttctac cctcatttca tctattatat gtttcaattc
     7021 tataacttca gaagaagtta ttctctttga agcaagtttt gcaatctcaa tttctaggat
     7081 tattctaagc tctaatatat cttcaagtgt accattatta agtttaaaaa tcatagataa
     7141 aggttctatt aaagtttttt ctatatttgt acatataaaa tttccttcac cctgtctgct
     7201 ttctatcact cccatagtct ctaataccct aatagcttcc cttatggaag ttctactcac
     7261 attcatttgt tcagacagtt ctctttctga tagtaattta tccccctttt ttaactctcc
     7321 atttagtatt ttatattgta tttgctcaat tacttgttca taaacttttt tactagctat
     7381 actattaaac attttttcct ccaatatgat aatcgttgtt gtaatatata tctattatat
     7441 atcttaagcc attacattca tatattttta ttacttttaa tataatatct tataactcaa
     7501 atttacaggt gatattatga aaaaaaataa aattattgct ttttttgctc catctatcat
     7561 agttggagtt ctagttttag ggattatcct atacccaaat gaagctataa aatctgcaag
     7621 agaaggcttt tctatatggt ctaatgtttt agttccatca ctactaccat ttataattgg
     7681 agcaaactta attgttgatt taaaaatagt ggatattata gggtttatca tcaatcccat
     7741 aactagattt gtatttaatg tttcaggaaa gagcgcttta gcttttgcta tatctacagt
     7801 atctggttat ccagtaggag taagtttagc ttctgaattt aggagtaatg gacaaatttc
     7861 taagtatgaa gcacaaagac ttgtgtcttt ttgttctaca tcaggacctt tattcataat
     7921 aggttcggtt ggtacaggta tgttccaaaa ttcaaactta ggttatttaa tgattctctg
     7981 ccattatcta ggaactatat tggttggtct tttctttaga aattatggta atgaaaatct
     8041 tccaaagaca aaattggatt taaaaacaaa tataaaaaat gtaatcaaca ttagaaattc
     8101 aagtgggaat ggtttttttg ttttatttgg aaatgctgta tttaatggag taaatacatt
     8161 acttacagtt ggtggatttg taattgtttt ttcagtagta tttaaaatac tatctctttt
     8221 taatgtaatc tcattaatag catcagttat atatttgcct ctttcatttt taggtgttag
     8281 taaagaactc tgtcaagcat ttgtaagtgg tctatttgaa attactatag gatgcaataa
     8341 agtatctagt ataacaagtt ctccagaaat tttaagagca tctttagcta gttttttgat
     8401 aggatttagt ggattatcca tcctagctca atgttgtaca tttctatcaa aaacggacat
     8461 agatttaaaa ttatacatac taagtaaatt tgcgcatgga gttttagctg ctatctttat
     8521 atttatactt tatccaattg caaactctgc tgtgttggtt tctagctttg ctgatacata
     8581 caacgttata tataataatt taatctggtt ttattatctt agctattatg atacaattct
     8641 tcaaattgta gtaataattt atctaatctc agccgtattt attgcaaaaa aacatatgaa
     8701 aactttatct acaaatggga aactaatgcg atataaaaat acttttttca ctaagaaatt
     8761 ttcacgaaaa aaataaaact aaaaataaaa ctaataaaac ttaactaaat acttccattt
     8821 ttttattata tttgtcataa ttttaataac ctttttaatt agtttaacag catagttatt
     8881 tcatcacaat ttaataattt tattagaaat atttttcaaa aaattctcct taatcacata
     8941 tatcacactt gtataagttt actgggaaat ttttccatta tataatattt catttatatt
     9001 atataataaa taccgaccta aacttaccaa atcaaactaa gaggagtggt tgaaaatggc
     9061 aacaagacct atagaaatag acgaatataa aaagataatg gaactattac atactggctt
     9121 tacttatagt gaaaatggcg ttgaaaaaag atttagaaaa aatcctaaag ttgcattggc
     9181 actaatgtta gaagctaatt taggtctaag aatatcagat attttaagat taaagatagg
     9241 taattttaaa ggtaatatgt tggaaataaa tgaaaaaaag acgggcaaat tacagtatag
     9301 accagttaat aaaaatataa ttgagtccat aaatgaacat gcaagaaagt ataatcttaa
     9361 atcaaatgac tacttagtaa atataaagac aaaggctatt caaaaacagt tgaggataat
     9421 ttgtaaatac ttaaatctgt ataatataag tacacatagc tttagaaagc tctatgctac
     9481 tactcaattt gaaaagagta acaataattt agagttggtt aaagaacttt taaatcacag
     9541 ctctgtagcc actacacagc gatatataag ggtaactcag caagctatta atgaagctag
     9601 tgaaaatttc tttattggtt aattataacg tatacatttt tctattatct tattaattca
     9661 ccagatatta aaaaagagtg tctcaaaatg aactttttag ttcatgagac actctttttt
     9721 tataaaatca aatatatttt tattatttca acactggaaa aaaggcttat ctctctattt
     9781 tgagacaccc tattaaattt tcataattct tgcataaaca tattaatcta tcttcaataa
     9841 ttttttagct gattcactgt aattatttct ttttacaatt tccaaaactc tatcaatctc
     9901 atgttctgac tttgcactta caaaaatcgc caattcttca tcatctataa tatcataact
     9961 atctacatat ttatctaaac attctaaaat tccatcttta ttattatacc caccaaatct
    10021 aactactgca ctacactcat cttctataag tccacttaaa tccaatttcc cttttgaaaa
    10081 agactttata tttgaacttc tattatctat tatatctata atatctgcat aaacagcact
    10141 tcctgttgca cctcttccag ctcccttacc tacaaaagaa agttcaccta ctgaatcacc
    10201 tactaaaata actacgttga actcattatc tatctttgaa agcatattat tattgtccac
    10261 taatgctggt ctaacaaatc cacttacttc tcctgattca tacttacttc tagcaaccaa
    10321 ttttatctta caatttaatt tttttgcata ttctatatct ttttcatcta ttgaagttat
    10381 tccttctaat aataaatctt tccaatatac tatttttcca taagataaag tagacaatat
    10441 agataacttt ctagcactat catatcctaa aacatctgca tctggattaa cttctgcaaa
    10501 tcccaactct tgtgcctgtt ttaaagcgac atcatacggt aaattttcat catacatctt
    10561 tgataatata aaattacaag tcccatttaa aatagcgttt atgctcttta tattgttgcc
    10621 ttccagtgat tctatcaatg gttttaaaac tggaatacca cctgccacag atgcttcaaa
    10681 cttgatgcta acattattta agttagctaa ttctataagg tcactacctt tttcagccag
    10741 taaatcttta ttagctgtca ctacatgtat ttttttattt aacgctcttg ttacataatc
    10801 aaaagctgga tttaatcctc caattacttc aactagtata tcaatatcct tgtcaaatat
    10861 atcttctata tcagtagtta tcttatcaaa atacttctta tacttgtgtt tttctttatt
    10921 tttaactaaa attccaacaa cttctatatc tctttttgaa gtattatttt ctattaaatc
    10981 aattaatcca cttcctactg ttccataccc taatattgct atcttcactg taacataccc
    11041 ccatcatttt gattatatta aattatttat atatataaat ataccaataa aaaaagcttg
    11101 agtataaact caagccatat gacgggttta tactcatctt tcagcataaa tgctgcagga
    11161 tttagcacca atgtataatt aaatatactg gttgccgggc ttcacagggc cagtccctct
    11221 gccactcttg ataagattgc taatttaaat ttatgtttaa tagtataata caatttcttc
    11281 caattgtcac tattttttta acttatttcc ctaaaaggat ataaactatt atctaacagc
    11341 tcttttagtt ggtttaccct tttttgtaac attattattt ttagtctttg ctttcgcctg
    11401 ttcatgtcct ggaaaaagta ttttaggctc ttcaatattc ctcttgtaaa tagtaaattt
    11461 aaaaccaata gcctgcacaa attcagctct taaagcttca catactgcat tagcagtttc
    11521 tttagtatca agacctgcat tttctaatat agtaactttt actaattctc tagttcttaa
    11581 cataccatct aattgctcta agaaactctc tgtaacacct tctttaccaa tttgtgtagt
    11641 aggctttaaa gtatttgcca aagatcttaa atacgccctt tgttttcctt ttaacatatt
    11701 ctcacctcta taattctata cttaaattat ctataatcta tatttaaatt aattgtaaaa
    11761 ctcaaattct aattcataaa ttttaaccac atctccatct tctattccca tttctctaag
    11821 cctatcaaaa acaccttgac tctccatagc tttttggaaa tattgaaggg attccatatc
    11881 atcaaaattt acagaataca ttattcttct aagtgcttta cctgtaacta catacacacc
    11941 atcttcaata tctatactta actcttcttc agtaccaata tcaagttctg gtctatacat
    12001 ttcttcttct gaaactagct ccacatcttc tacttctttt agcattttag aaacataggc
    12061 tattacttca tctatgcctt gacgagttgc tgctgacatt ttaaatactg tatatcctct
    12121 gccttctaat tcactcttaa acttctcaaa cttactctca tcttctaata tatcaaattt
    12181 atttgcaact acaacttgtg gtctctttga aagcttttca ttgtacaatt taagctcatc
    12241 atttatcttg tcaaaatctt caatagggtc tcttccctct aatccagata tatctacaat
    12301 atgtatcaat acctttgttc tctcaacatg tcttaagaac tcatgaccta atcctatgcc
    12361 ttcagaagca ccctctataa ttccaggtat atctgcaagt acaaaactat ctccaaactt
    12421 agtttgaaca acacctagat ttggtgtaag tgttgtaaaa tgatagtttg caattttggg
    12481 tttagcttta gttacaactg atagaaatgt tgatttacct acatttggaa atcctaaaag
    12541 accaacatct gctatcattt taagttcaag agttatccat ctttcttctc catctgtacc
    12601 agattttgca aaagctggag cttgtcttac tgcatttgca aagtgttggt tacctttacc
    12661 acctatacca ccttttgcaa ctatagctgt atctccttct ttttttaaat ctgctaatac
    12721 aagacctgta gcctcatctc ttattactgt acctgctggt acttttaata ctaaattttc
    12781 tccattttta ccagctcttt ttcctttgct tccgtcgcca ccattttgag cttgatattt
    12841 tttctggtac ttaaagtcca ttagagttct aagccccaaa tcaacttcaa atatgatgct
    12901 tgctccacga ccaccatctc caccgtctgg accaccagct ggaacgtatt tttctcttct
    12961 aaatgctacc gaaccatttc caccatttcc agccttaaca aatattcttg ccttatctat
    13021 aaacaaattc ttcacctact taattaaaaa cttttaatta gcattaccaa aaataccatt
    13081 ataaattcat cagtattata agaaaaagga gtgcctcaac actcctttta tgtatattac
    13141 tcagctattg ctactggata tatactaact ttttttctct tcttgtcttt tctttcgaat
    13201 ttaacagtac catcaactaa tgcaaataaa gtatcatcac taccttttcc aacattagtt
    13261 cctggatgta ttttagttcc tctttgtcta actattatac ttccagcagt tactaattga
    13321 ccatcgaatc tcttaacacc taatctttta gatatagaat ctctaccatt tttagaagaa
    13381 cctacccctt ttttagatgc taataattgt aaattcatgt ttaacatatg gagtcacccc
    13441 ctactttttt aaaattttta tattttttgg gtattcccgt ttaatttctt taaccgctaa
    13501 ttgaaagtga tttagtaata attgtgcgtc ttttacatgt gtatctttga ctatacactc
    13561 tatatgacct tcttctccta ctactttttc aaattcaatc ttctgtaatt tgtctaaaga
    13621 atttataact gcaatagcat tagatgtaac tgcagcacat actatatcat agcctatttc
    13681 agcaaaatca gcatgtccct ttaaacaaaa accttgcaac aagaaatcat cattataata
    13741 atatttaact tttatcataa cttaaccaat aactaagcgt ttattttttc tacaactatc
    13801 ttagtgtatg attgacgatg tccttgtttt cttctgtaat ctttttttgc tttatacttg
    13861 aaaactataa ctttcttagc tttaccttgt tcaacaactt tagcttgaac agatgctcct
    13921 tctactactg gagaacctaa ttttaattct ccatctttag agcaagctaa tacttcgttt
    13981 aaagtaacaa catcacctgc gttagcttct aatttttcaa cgaataatac atcaccttca
    14041 gaaactttat attgctttcc accagtcttt actatagcgt acattaaata cacctcctcg
    14101 gcctcaaact cgccagtaca aggtacttca tttgaagatt taaaccttta ctgtgcggca
    14161 ttacaattat taattataaa atatttacat tttattgtca actattttat cgaaaataac
    14221 attggtttta ttgtgcttta atgtactatt tgcgtttaaa gaaatcttta tattatattt
    14281 ttcaccaatt ttatcaatta tatccatata atattctttt atttgttcaa aaataaaatc
    14341 attaaattct accattatgt ttttgtaaac agtatgttca cttattctca taatttcttt
    14401 ttcaatacta tctagtatat aatgtacaga ttttattgtt tgttcaccat cacaacatgg
    14461 acaagacatt aaataatact tatcaatgga ttctttttct ctacgcctag caacttctac
    14521 taaaccaagt tttgtcatac ctaatacttc agcttttctt ttatctttat taaattcttc
    14581 ttctaggatg tgtaaaatct ccttttgatg cttcttttta tgcatatcaa taaaatcgat
    14641 tattattatc ccagctatgt ctctaattct aagctgtctt acaatttctt ttgcagcttc
    14701 aagattagtt ttataaacgg tttcatcaag tttaccagtt ccaataaact tacccgtatt
    14761 tacatctata accgtaagag cttctgtttt ttcaattatt aaataacctc cactctttaa
    14821 ccatactttt ttatttaata atttttctat tttagattct attctgtata aatcaaatac
    14881 atctctattt tcctcaagac acagtttatc aacataagtc ttatctatac ctcttaaaat
    14941 agacttaagt tcagagtact tatcataact attggtaatt atagattcaa tatcatcatt
    15001 cacattatct tttacatatt tagttgcaaa atctaaagac ttatataata atttcggacc
    15061 tataccaagt ttatattctt ttaaaatgtt ttcatacttt aatttcaact cttctatatc
    15121 tttctttatt tcatcatgag tgcaaccttg agcctcagtc cttattatta atcctagatt
    15181 ttccttattt actgccttag taattttttt taatctaaat cgttcttttt catcagtaat
    15241 tttatttgat attgtagttc tatcattgga aggtatatag acaatatacc ttccagaaat
    15301 actaatctca gtgtttaatt ttgcgccttt agttcctatt tcttctttat ttatctgaac
    15361 caaaatatcc tgaccagatt ttatagtact tccctttttt aattgtaaat aagcaagttt
    15421 cttaaatcca atatctacaa aacaagcttc tattcctggt ataacttttt ttacgattcc
    15481 acgatatata ttggatacag tcttcttatt taaattgtct tcaacaaaaa gttctgttaa
    15541 tctttcatct tcaagtactg ctgttttctg ggaccctatt agggactcaa taactatctt
    15601 tttcacaact tcacctctcg agcttacata ggagtcacta attccccatc ttctaataca
    15661 tataaatctc ttctcaatat atcaacatct aatgggtcaa tatcaagttc aaacatctct
    15721 aatatctttg gtataagtat attagtattt aaattagttt ttgagcctgt agctatagta
    15781 gcagtaagag taatatgttc atcttctaaa tttaaaacat caaaggttct tatcataggt
    15841 cttatatcta cttcaaccat cttacctttt ttattctttt ttgttatgat tatttcttta
    15901 ttattcataa agtcaattac ttttcttttt acaaactctt tcgtcatagg tcttttcaaa
    15961 tctatattaa acaaatattc tccatagtct atcacagaag aaagagaagg tgtttgtcta
    16021 tcaatttctt ttgctttgat aaaatctatc ccatctggaa gttgaattga tactttattt
    16081 aaaaattcct ctactgataa atcatcttct tctatttcaa tatctacata ctcaccttga
    16141 ctttctgccc ctaaagccaa agcattgcca taactcattt tagggtgagg attaaatcct
    16201 tgagaatgtg ataagtttat ttcagctctt ctaaacgctc tttgtaacaa tctctgaagg
    16261 tcaagatgtg atatatatat catgtcgcct tcctttttaa attttactct tattatctta
    16321 ctcatattag caaagccccc ttcctatatc atgtatattt attccacaac cattgcagtt
    16381 atgtctacaa tcagaagtga ttttttcttt tttagcattt tcgtcttctc taattaagaa
    16441 ttttttacta atacctacat ctatatggtc ccaaggaaat acctcttcat agtttctatt
    16501 tctatgtgca taaaaatcta tagaaagatt attcttttcc atagcttctt tccatatatc
    16561 taaattaaaa tgttctgccc atccatcaaa tttagcacct agtctaaatg catcatatat
    16621 aacttttcct attcttctat cgcctctagc tactactgct tccatcaaac tagtctttga
    16681 atcatgataa ttgtatttta tattgttatt tcttaattta tttactaaat gtctctgctt
    16741 attaataact tcttctgtag tatcttgacc atgccattgg aaaggtgtaa atggctttgg
    16801 tacaaatgta gaagtactca ctgtaacact aaaacttctc tttagctttc ctccatttac
    16861 ttttctataa atatcaataa ctttataagc caatttagct ataccatcta aatcttcata
    16921 agtctcagtt ggtaaaccaa tcataaaata caacttaaca ctattccacc ccatctcaaa
    16981 agctctttct gttgcatttt ccaaatcttc ttctgtaaca cctttattta taacatctct
    17041 aagtctttga gttcctgctt ctggagcaaa agtaagtcca gattttctta cttgttgtat
    17101 tttatcagca atttccattg aaaaattatc taatctaagg gatggaagcg aaatcccaat
    17161 attattagat gcatattcat ctaccaaata atcagtcaac tctgataact tactataatc
    17221 actcgtacta agtgatgata atgatatttc atcatatcca gtattcttaa ctaatttatc
    17281 taatatttct ttaagtcttt taacactctt ttctcttatt ggcctgtata tcatacctgc
    17341 ttgacagaat ctacatcctc tagtacatcc tctaaataat tctagtacta ttctatcatg
    17401 aacagtatct atataaggta ctattagttt ttctggataa tctacagtct caacatcttt
    17461 tattatcctt ttagtaggat ttttagtaat cccttctcta ttaggagtta cactttgaac
    17521 tgtaccatct tcattgtact tgacatcata aaaacttggt atgtatactc cttctatgct
    17581 agatatttta tataaaaaat cttctctagt cgttttattt ttcttccatt ctttatactc
    17641 atttactact tctaaattaa cctcttcacc ttcacctaat attactacat ctacaaaatc
    17701 tgctaatggt tcggaattat aagcacaagg accaccaacc attataaatg ggtcttccaa
    17761 agttctttct tcttttaata ttggtatatt ggctaaatct aatatattta agatattagt
    17821 gtatgaaagt tcatattgaa gtgtaaaagt gacaaaatca aagtttgtta taggctctct
    17881 tgactctaat gcaaataatg gtatactttt ttctctcatt atattttcca tatcaactgc
    17941 aggagaatac actctctcac aaaatacatc ttcatcctta tttattacat catataatat
    18001 atgactccct aagtgactca taccaacttc atataaatct ggaaagcaat gtgcatatct
    18061 tatcaattca ctattactag tatctttatg aattgaattt atttcatttc caagatatct
    18121 agcaggcttt tctacttttt ttaagatttt tttcaagtct actttattca tttattaaaa
    18181 ttcctccaaa caagctcttt atttttattc ttatttataa gtaatattat acacgataaa
    18241 atattttcta tcaaaaatat aactaaaata ttgaatttaa aatacatatt attcacaaaa
    18301 tagaatagct aaaaatattt aagaatattt tctattaaat ttttattgaa atacaatttt
    18361 tttacaaagt atgatatatt ataaacataa aactattgtt ttacatgact tatcatgaca
    18421 tcttttgttt cattcacctc aaaatggggt atgttactta tataatgata aattttagga
    18481 gggttaaaaa tgagtaaagt ttttgaagta aaaaaagata tctactttac tggagtagta
    18541 gatgaaggtt taaaagtatt cgacataata atggaaactg agtttgggac aacttacaac
    18601 tcttatttaa tcaaggacga aaaaacggtt ttatttgaca ctgtaaaggc aaattttaaa
    18661 gatgagtttt taagtaattt atcagaggtt actgatatag ctaaaattga ctatgttgta
    18721 attcatcaca cagaaccaga ccatgcaggt agtttaaaat acttattaga tataaatcct
    18781 aatatagaag tttattgtac taaagctgct aaactatact tagatggtca aataaataga
    18841 ccttttaact gtcatgtaat aaaagatggt gaaattttaa atatcggtaa aagaaatctt
    18901 agatttataa ctgctccatt cttacattgg gttgatacaa tgtttactta cattgaggag
    18961 gacaagactc ttttaacttg tgatgcattt ggttgtcact ttgcaagtgt tgatgcagag
    19021 gtagtgaata gtgaagatta tctaaaatca gctaaacact actatgattg tatagttaag
    19081 ccatttgcta aacatgtttt aagtgctgta gataaggtgg taggactaaa catagaattt
    19141 gatactatat tgacttctca tggtccaatg cttactaaag acccaatggc tgctgttaaa
    19201 agatatgttg agtggtctac agaagctgtt aatacaacta atcaaaatca agtttctata
    19261 ttctacttat cagcttatag taatacttta gagatggcta aaaagataaa agaaggactt
    19321 gataaagaag gtgcaaaggc tgaattatat gatttagaag atatgacatt aacagaaatg
    19381 cacgatacat tagtagtgtc aaaagtaata ttactaggct ctccaactat aaacaaaact
    19441 atggttaaac ctatgtggga tttattctct gttatagacc caatggctaa ccaaggcaag
    19501 atagcaggag tatttggttc atttggttgg agtggtgaag gtataactat ggctgagact
    19561 ttacttaaat ctatgtcatt caaaatgcct gtagaatctc ttaagaaaaa attcttccct
    19621 agtgaagaga cattaaaaga gtgtatggca tttggtgcag aatttgcaaa attagttaaa
    19681 tagccattaa tcaaacataa tattatttaa aataaatgat tttatattta aaaataaatt
    19741 aaaatggagt tgtctcaaaa ttaatttttc taatctaatt ttaagttcaa ctcctttttt
    19801 attttccaaa atgactaata taataataaa tgtacccttt cgcacagggt gcattattta
    19861 tagtctgttc tatttgtata ctagtatact tgtattaatt cacgtatcta gtatatgcta
    19921 ctagccctat tccagaatta gatgttgttt caccaatttc tactttaata attatattat
    19981 ttttttacac ttttttgata attctgctgc agaaaggttc ttaccagtaa aatctaaaga
    20041 aaatgcctta tcatactcaa aaggataact aacaggtttg ctctctggtg ctaatgcaac
    20101 cataccatat ggtgtattca taaaccctat tacagtaact gtatgctttg atgggtctaa
    20161 atttgaattt ttgcttgtaa acttaattgt atttattgat gagttgatta aactacacgg
    20221 aactccctca tacatttcaa tttttggacg tagattattt ccataaactt catcaagtat
    20281 tgccccttgt tgatctgcta tcatagtgtg attcccaggt ccaactggcg cagaaggctt
    20341 agctggcgta ggtttcttaa cagttggcgt tggattctgt gtctttacta cttcgccaac
    20401 agctggatat gctttcttgt caaatggtaa tgggtgagta agactcttgt atatgatgtc
    20461 tgctcctgct gatttgttgg catcaatatc tacatgatac aaacgtccat cctcaaaacg
    20521 aataagattc cactgatggt catttgttgt actatgtaca taataacatt caattcccat
    20581 agcatcacat aaagctttca agctcctaca cattccagaa caagcacctt ttttagtaac
    20641 caatgtataa gcagtattgg ctccctctgc tgcgttccag ttaacatatg ttgtgttgtt
    20701 tttaatgtga ttatgtatag atacaatcca ctatggagaa atataaaaga aaagaatata
    20761 acaaacaata aaaaataaga tgattcaaat tttattttga aacatcttat ttactctaca
    20821 aattaaaatt atttggtgaa ttatttaatt attatttttt attaaagaaa ttttaaatct
    20881 taatataaaa tacgctatta tacataataa tccaaatacg aatgctactg gaattaacat
    20941 ctccgattca aaataaaaga aatattctaa gaaaatatct ggatttacta tattcccatt
    21001 cttgtctaaa aaatcttttc cattatatcc taaatgctgt ataaaattac ttatgttatt
    21061 ataatctgct tccctctcat ataccatatt agcattcaat ccagaagaac ttaaattagt
    21121 gtttccatct ttcatatcta ttacatatag tctaagagct ttgactttac tatacttatc
    21181 tttatcaagt attttttcaa gttctaactt atattcacca atctttgtca tatcatgtct
    21241 ttctgccaat tgctttattt ctccatcaag ttcttttcct aaattataca tattatgttg
    21301 taaactatta ttgtaatacc acataaatcc agaaattaaa aatgcaatta tacttatgta
    21361 acctaatact tttttaaacc ccattacaat tgatttatgc ctatccaatg ataaagatag
    21421 ttcttttatt atattacata attcatactg catttcatca ccatcatcaa atctttcaat
    21481 tgctttttta catgcttctt cttcatctaa tccttctaat ttaaattcat ttgcagattc
    21541 tataagatgg catctcattt cctgttttaa ttctatagta cttttattgt cacatttttt
    21601 atatagttta tctaaatatt catctattag tttcataaat taatccacct ctcccaaaaa
    21661 ttgattcata acctttttaa taaatctcca ttcttgtttc tttatttcta aaaaatcttt
    21721 accttcatta gttaaattat aatattttct tcttccacct gaactttgtt cattgctata
    21781 ataagattta attaaatttt tagattccat tcttttaagt gcaagataca ttgttccttc
    21841 ttttaattca aactcacttc tttctcttac ttgctttgca atttcatatc cataacaatc
    21901 tttcttttct aatatagaaa caattaatat atcgatataa ccttttaaca cttctttatt
    21961 taactgcatt atgtaccacc gccttttcac ctactactct ataatgtata gtacttatat
    22021 ttactataat aatacatagt actctataat acaaggttta tattctacaa tagacttaaa
    22081 aataataaat agctgtatat taatatttaa tctataaata ctaatataca gctatttatc
    22141 aaattttaaa aataaatttt tgttttatta atgttgtcat aacaacattt acttggttga
    22201 aatagtatta cctataagtt taaatatata taataataga cttgattcta tttcatatta
    22261 ttaaaataga gtaatagttt aaattctact actctatcta taatctattt cctaattatt
    22321 taatctcaaa tttagctctt catcatttaa atctttcata agatttctac aggtactaga
    22381 tttattccaa cagaaaaact agggcattaa acacttatat atctgtttct ttagactgta
    22441 atattaatat tttttaaata cgttgacttt tgctttttct aacttctctc catgatagtt
    22501 ctcctctatc aagagctttt ataaatattt ctgctgaagc tagatttgta gctattggaa
    22561 catgataaac atcacaaagt cttattaacg cttgtatatc tgtttcatga gcctgagatg
    22621 tcaatgggtc tcttaaaaaa ataactaaat ctatttcttg tgttactatt aaagaaccta
    22681 tttgctggtc tccacctaaa ggaccagatg ctaatctatt tatatttaat tcagtttcat
    22741 ccattattct ttttcctgta gttccagttg catatagtcc atactttttt aaaattgatt
    22801 cataacctat acaaaatccg accatagtgt ttttcatttg gtcatgtgct actaatgcta
    22861 tattcatata tctaatctcc tcaatttatg tttagatgta tttaaattta gaaatttatt
    22921 ttctttcttc tcatacacac atttagtact aatcctaagt ttgctaaaga tgtcaataac
    22981 gaacttcctc cataacttac aaaaggtaat gttactccag ttactggtat taacgcaacc
    23041 gtcataccta tattttggat tatctggtaa ccaaacattc ccataacacc aaccactatg
    23101 agggttccat aaaaatcttt tgcatctctt gcaattgcta aaagccttaa taggaatatc
    23161 ataaataaga ttatcaacac tgccattcct attacaccaa gttcttcacc tacaacagca
    23221 aatataaaat cactatcttg tacaggtaaa aaatcttctt gattttgact tccattgtaa
    23281 agtcctttac cagttacgcc tccagaacca attgcaatca gagattgcat aacttgatag
    23341 tttcctttta gagttacatc ttctggattt agaaaagctt ctattcttac cttttgatgg
    23401 tctgccatca ttaaatacat cagtggcatt gacactaata gtatgattat tcctcttttt
    23461 atcaactttg aactaagacc tgctgtgaac agcattgcaa atatcataca acaaaataca
    23521 attcctgttc ccaagtctgg ttgtgcaatc aataatccga taaatggaag agaataaact
    23581 acaacaggca taacttcctt taacgtattt aatttatcct ttttggattc aagtatcttt
    23641 gcataactta gtacaaatgt taatttgact atttcagatg tctgcaagtc aagtggtcct
    23701 aagttaatcc acgacctagc tccacctttt actgtcccaa ttccaggtaa taaaactatg
    23761 gctaacaaaa taagacttat tatataaagc gctttataat acctgcctag aaggttatag
    23821 tcaaaaaata atatgactat aatcattcct ataccaagta caaaagctaa tccttgttta
    23881 atcaattgat tatatgaacc tgttgaatta gcatgtgttg cactactaag tataacaagc
    23941 ccaaaaataa agatagctaa tactgtaact ataagtttcc agtctaactg ttttatcaac
    24001 ttaattgtcg acttataatt aatattgatt tttttcaaag aatcaccgtc tttctatctt
    24061 taaacatata attaaataaa aggggcatat accaataagg atatccccct tttaagcact
    24121 aattacatac atctatcttt tatattcttt agtggtatat tagcaactaa tgcagataca
    24181 ggtttgtcat catgctcacc tctgcattta gacattttta tttcaagacc atcatcttct
    24241 atttctgcat aattagctat tactttcaaa atgtctgttt ttatcatctc taaaagttgt
    24301 ggtgaacaat ctactctatc atgaactaaa actagcttta acctctcttt agcaacagat
    24361 ttactagttt tagcctcgtt agaaaaaact ctaaataaat ctaacacaga tgtatcctcc
    24421 tttgctactt agccatgcta aacatttttt taagtctacc aaagaatcca tcttcaactt
    24481 caagatcaag tagaggaact tcttcattta gtatcctttt tgcgatattt ttgtatgctt
    24541 gaccagcaag tgacttagaa tcaagtatag ctggctctcc tttatttgtt gatataatta
    24601 tgctttcatc atcaggaaca agacctaaca aatctattgc taatatttct attatatctt
    24661 gtttatccat catgtctcca cgcttaacca tatcatttct aattctattt ataactaatc
    24721 ttatctcttt tatttcattt gcttctaata gacctattat tctatcagca tctcttactg
    24781 ctgatatttc tggattagta actacaatag ctctatctgc cccagctact gcatttttaa
    24841 agccttgctc aatacctgct ggacaatcta ttattatata ctcgaaagat tcttttagtt
    24901 tctcacacag gtcaatcatc tgctcaactg agacagcatt tttatctcta gtttgtgctg
    24961 ctggtaataa atatagatta tcaaatcttt tgtcttttat tagagcttgc ttaagcctac
    25021 aagttccttc aactacatct actatgtcat aaactattcg attttcaaga cccattacta
    25081 catctaagtt tctaagtcca atatctgcat ccacaactac tgttttttta ttttctaggc
    25141 ttagcgcagt tcctaagttt gctgcggtag tagtttttcc aaccccacct ttaccagatg
    25201 ttataactat aacttcgctc ataatactac cccctatttt tctttctatt tgtctaactt
    25261 tgacaaatag ctttcgatta caattcttcc attacttaca aaggctattt ctgggctaat
    25321 ttctgattca ctttcataat tttcttcatc atctggagct tctgcaatat tatctgctat
    25381 ttgtaatatt ttaggattaa gatttccagc aacaacatat gcaaattcat tgccttctgc
    25441 acctgcatgt acaaaacctt ctattttacc cattacaaaa acattacctc ctgctacaac
    25501 cttagcacca gcatttatat ctcccataac aactgtattt gattttgaaa ggacttttga
    25561 accaggtttc atatcattca atataactac atcaccttca aattcaatat tttcaccaga
    25621 tcttagggta ttgacatatt ttgttggaaa attactattt tcttcaattt tttgatcttc
    25681 aacaaattcc acatcaaatc tagaagtaat tccttcttta agttctagaa tctccatgtc
    25741 agttaaacaa tcactattaa ttttactaat tttagcccca ttaaaaaagc caacatatgc
    25801 ttccaatttg tttattattt tttcctgtat ttcctcaaat ggagcttctc tttttatatt
    25861 tactattatc cctcttttat tacccttgaa ctctactagt tcttgactac aaatttctct
    25921 taaagacata acaacctcca gagtgatata tcatttatat tcttcaaata cgctttaaac
    25981 ccttttattt tttctatata aagctaaagt ttatacatag attgagctat ctattaattt
    26041 gtgactcgaa atttatattt tcgtcagaag aattgttaac atcattcgtc ttgttgtcat
    26101 ttttactatt agttggctct aatcccatat aagttccaat aacttctcta gttggtaaaa
    26161 gtgagaatac acttgaatca ccttgaggaa ttacacatac aacagctatt tctgggtcat
    26221 ctgctggagc aaaagcaaca gtccacgtaa aagaaccata atcttgttta aatctatcta
    26281 tttggtcatc tgttattttt ggatttaatt cttttatagc ttttcttaga tatgaagaat
    26341 tcaccttatc agtgtcctct aatctaactt ttactccatc ttctaattct tcctctaatt
    26401 tctttctttc ttcgtctttc aaatctttat tttctaattt cttttttatt tccttttcct
    26461 tagctaatga aagctctttt tctttttctg ctttcatctt atcagctagt ttaatcgcat
    26521 catttaggtt gacattataa gatgacatat gactttttaa atattcatac tcgttatctg
    26581 taggtatttt cccacttttc tcagctgtac cagtttttgc tgctacatct attggaaaat
    26641 cagcaaaagc actcttcgct gtaccttgtc ttgcaaccaa tttcatacct tttgttaatt
    26701 cttttaagtt atctggattt ttaaaaggta ttttttcgac tttcttctgg tcattaattt
    26761 taactgatga ataatcactt gaaacagctc tatcaactac agataattct actaaattac
    26821 ctccatttgc tatagcagct acatatcttg atatttgagc tggtgtataa gcattttcac
    26881 cttgacctat agctaagtta aatgtatctg cagtactcca ttttgcaaag ttaaaataac
    26941 taaatactgc taaatctgct acttcttcaa ttctatcttc tttaacatcc aattttttta
    27001 gcctattcat tgtttcaact ctaccaggag ttttcttttc tgctgcccag cttacaatct
    27061 cattgattct tttctcatat tctttaggat ttttttctct agttatatca acaaaatcat
    27121 ttgccatttc tctttctaaa tttgacttta gaagagcttg tgttgatttc aatttatctt
    27181 ctgtgctagg aactttacct gctctttctt ctacttcatc ttgaagccct gtattttggt
    27241 caagcccaaa taattttgca tactcaagta ttttatttgc attcatatct ataccaatgc
    27301 tttttccatc tggccatgtt ttacctgtac caattgttgc catatatata ttacaagact
    27361 cttgtattgc tttatataag tttgtcatac catggtttcc tctaccatgg ttccatacat
    27421 agtcaccaaa agatttttta cctaacatta taaccccagg gtcatttata gtaaagtttg
    27481 gatccagacc attttctaac gctgccattg aagttaccat cttaaatgaa gaacctggct
    27541 gaaattcccc ttgtgtaacc aagtttaaaa gtggacttcc tgctagtaag tcatttggat
    27601 tttttggttg aagtgcttta taatcctctg tagaaatacc ttttgcaaat ttatttgggt
    27661 cataattagg ataacttgat gaagctaata cttctccatt tttaacatca atagctataa
    27721 gagctgctga ctgtgctttt cctgcatatg cactaattgg tttgtcccca aatttacttt
    27781 taaaagtacc tcctttagaa gcaacttcga ttatctgttt taatgcattg tctgaaactt
    27841 cttgtaaatc tttatctagt gtaagataca cagtatctcc tgatttaggt tttttagatt
    27901 caatttcttt actgattcta cccaatgcat caactttaac cattttataa ccatcagttc
    27961 ctcttaatct actttcatta ctcttttcta ctcctgcaag acctaccata tctccagttt
    28021 cataaccttt ttgaaggtat gattctattt gagtggatgg catttttcct acatatccta
    28081 acatatgaga agctaatgac ccattaggat aatatcttac tggttctact gctattgaaa
    28141 caccaacaag gtccatagca ctttcttcaa tttgagcaat cgtagtttct cctacatcct
    28201 tagcaatagt tactggatta tattgagaat atccttttga ttttatcaaa tcccttacga
    28261 ccattatttt tcttgcatct tcatcactca aacttttatc aatttctaat gcatcactat
    28321 ttcttacttt ttcaaacgct tctttggcac tcagtttagt ttcttttatc ttataactag
    28381 ccaaccaatc tctcttatct ctttctgctg tgaacatcca tttactaact aaaataggtg
    28441 gataatatcc attttcattt agttttgcct gtaatcttgt cgcatccaat cttttatctt
    28501 cttgagataa tattccagct gatataagtt tgtccaccaa gtaataaaaa ctttccttag
    28561 cattataatc atttggaata ccattttctg atttatattc cctaatatct ctgtcatatg
    28621 tataatagta ctttccattt tctacatata taggaaactc atctacatat tcttcaccat
    28681 ttctctccag caaatttata agttttaatg atatttcatt tgctcttgaa tgcttatttg
    28741 catctttctt attaatatcg ttacctgaaa cttgaacagt aaataagttt ttatttcctg
    28801 ccaacagtct tccatatcta tcttttattt ctcctcttgg agcttctatt gccaactttt
    28861 tatatgtttt attctcagct aactcattgt aatattcata tttaaatgta gtcatataga
    28921 ttatttttac taaaatagct aaaaatatca cgcccatgac tatcttaatt atttttagtc
    28981 tatcaacttg tttattttct tccatgaaat caccaactaa tcttctttaa gttttaaaac
    29041 tgctctttta gataagcgat atattactaa accaattaaa ctattcacta ttggtgctac
    29101 tagtagacct tttacaaaaa gtgttgatat gttgtagctt tgataaatta tactagatgt
    29161 acctatattg acaattgaat caattaaact gcctataaaa attaaaacga aagtaataat
    29221 ataactacct ttatatagct gatctctcat gtatccaatt gcatatgcaa taataaacaa
    29281 aattaatgca tttacaccaa aaattccacc aacggttatg tctttaataa gccctattat
    29341 tgctgctata atcccacctt ctaattcatc taaataaaga gagattatgg ttacatatat
    29401 aagaactaaa ttaaagctta tgccaaaaat atctatataa tttgttatac tattttctat
    29461 agtcataagc acaatcccca acagacaaag taaaactttt ttcattgttt ttatcccctt
    29521 tatcctatat ttcttggttc tatgactaca acgtcattta tattcttaaa atcaacataa
    29581 ggttttacaa ctacatattt taatgactta tctttatcat caaccacttt ttcaacttcg
    29641 ccaattggta taccatctgg gaagaatcca agacctgatg ttgttattat atctccttga
    29701 attacattat atgatgagtc aaacatatat ccttgcaaat atcctttact attataacta
    29761 tctttattat ctaaagtagt attttgagta attgtacctt tagcattggc atcctttgct
    29821 agtttaaagc ttacagatgc cttagaatcc aatagtgaaa ttgccttact ataattgttg
    29881 gaaacttcat aaactatgcc aactaatcca ctaccattca taactatact ttcttttttt
    29941 acacctgaat tttttcctgc acctatgaca aaactctcat accagtttcc atcattttta
    30001 ccaacaacac ttgctgaaat acttgtcgcc ttatactctt cttgtacaaa gttcagtgtc
    30061 ttttttaact cttctagcga ttctgtttta tctagtttgg catttaaagc tatcatattt
    30121 tttttaagtt tttcattttc ttcttctaat ttttttacct ttttagaatt atctttaaaa
    30181 tttgctacat ctttaaaacc atttttgata aaagaaaatc catcatttat tcccttacct
    30241 acacttgtaa tagcatctga agctactcct aaatttactg gactaccact agaaaattta
    30301 ccaattgata tacccacaat tccaattaaa gtgatagcaa ctacgcccgt tgctatcact
    30361 ttaacattaa tccttttttt ctcatttttg ttcttgtcaa atctcaaggc catcaccaac
    30421 ttctaattct tagtattcat cattaatact ttttcaaaga tttcttggtc ttcaacagat
    30481 ttacctgttc caagtgcaac acaatcaaga ggtgtttctg ctatttgtac agacatacca
    30541 gtttcctgtg taataagctt atcaagacct ctaagcaaag cgcctcctcc tgttagcatt
    30601 attccgtttt ccattatatc tgaggctaat tctggtggag ttctttctag tgtagatttt
    30661 attgcatcta ctattgaact tacaggttct tttaatgctt ctctaacttc tgtagagcat
    30721 atttctattg ttttaggtaa tcctgagatt aaatctctac ctcttatttg catatttatt
    30781 tcttcatcat ctttaaatgt agaacctatg ttaattttta cattttcagc agttctttca
    30841 cctatcataa gattatattc tttttttaca taagccacta tagactcgtc aaattcgtct
    30901 ccacctattc ttatagattt agcagtaacg attcctccta aagatattac agctatttct
    30961 gaagtacctc caccaatatc aactaccata ttaccttctg gttcttcaac ttttaatcca
    31021 gcccctatag ccgctgccat tggctcttct ataaggaatg catctttagc tcctgcttgt
    31081 cttgcagcct cttctatagc tctcttttct acttctgtaa ctccaaaagg aacacatata
    31141 gctattcttg gacttacaac acctttttta tcagccgctt tttgtataaa atagctaatc
    31201 attgattgag ttatgtcaaa atcagctatt actccatctt tcataggtct tatagctact
    31261 atatttcctg gtgttctacc tatcattttt ttggcttctt cacctactgc taatacctct
    31321 ttactatcat ctcttattgc gacaactgat ggttctctaa caacaatacc ttgtcctttt
    31381 atgtaaacta atgtatttgc agtccctaaa tctattccca tatcctttgt catcttatta
    31441 aaactaaaaa aacctttttt ttctttcttt ttttccttag ccatgatttt actccttcct
    31501 actattaaga atcatgttta aaacattctt ctattatttt atcacatata aagtcaaatt
    31561 atcatatttt ctttaaagct aaagtataat ccatctccaa taattatatg gtctattatt
    31621 tctattccaa ttatttctcc acatttaata agtcttgatg tgatattttt atcttctctt
    31681 gatggttcta cacttcctga agggtgatta tgcatcacta ttatcttact tgcacttctt
    31741 cttatagctt ctttaaaaac ctctcttgga tggactaatg acgagcttaa agtccctata
    31801 gatacatcta catcagatat tatctcattt tttgtgttta aaagaaccgc tttaaaaacc
    31861 tcttttttta aataccttag gctatccata taatacctct gtatatccca aggattcctt
    31921 attttatact ttataggttt aaaactagct actctagagc ctagttctaa agctgcttta
    31981 atttgggttg attttgataa cccaattcca tctatattac acagctcctc tattgtcata
    32041 tcttctaaat gtctaatacc ctgaatatct ctatttatta tatagttggc taattcaata
    32101 gcatttttat tcttgttgcc agttcttaaa agtatagcca acaactcagc atttgataag
    32161 ctttttacgc ctttggcaag catcttttct ctgggtcttt cctctggtgc catttctttt
    32221 accttaatgg ttgaattaaa tgactttttc agataaatca cactccataa aaaagattta
    32281 tactaaaata tttttttaat aggtcgccta accttgaaat tggaagacca actatattaa
    32341 aataatctcc tctaatttct tcaactaaca aagctccata accttgtata ccataggctc
    32401 ctgctttgtc aaatgactca ttagttttca gataatcctc aatatcctgc tctgataacg
    32461 ttttaaattt cacattgctg attacatagt caattacctt tttatcttcg cacaagttta
    32521 ttaagctaat acctgttatg acttgatgct ctcttccaga tagtcttttt aacatatctc
    32581 ttgcacaaga ctcatctttt ggttttccta aaatagcatt gtctaaaaca acaactgtat
    32641 cagctccaat aacaacatct tcattatgtt cagatgctac agacatactt ttttcaaaag
    32701 ctaatctcat aactaaatgt tttggtgttt caccttccaa tataatctca tcaatttcat
    32761 ttttaattat atcaaatctt acattcgtat tctctagaat ttctttcctt cttggagagg
    32821 ctgatgccaa gattatattc atttattcac ctcatttgta cgtaatttgt acctatttag
    32881 tctatcacta tattgtaaaa aattcaacag aaatgtcgaa ccttttttgt tacactattg
    32941 ttatatttat taccattaga ataaatatac ataaaataga ggtatggaaa ttattccata
    33001 cctctgtttt atgtactcat tagagatatg gaaattattc catatcctta ttttgtccat
    33061 attacaaaat tttaatagct ttctttggac atttttccat acataaatgg catcctacac
    33121 atttatctgc atctacctta tgtttttctt ttaattctcc ctctattgca tcaaacttac
    33181 attgtttttt gcacactgta catccaacac aaagttcttg gtcaatagta actttctttt
    33241 tcttagttat gtcacctgat attacttttg tagggcattt tgctacacat tgcatacaac
    33301 ctacacactt attagggtct atcttagcta ttttgtcttc aaatattatt gcatcaaatt
    33361 tacaagcttt tacacacatt ccacagccaa tacatccagc agtacatttt tctttaacag
    33421 ctttaccaaa ctctttgcta ttacactcta caactacttc ttgactttct ggttttgtga
    33481 ttattatacc cttaggacaa acttctttac attttccaca atttacacac ttttcttcat
    33541 ctattactgc tattccatca actatagaaa tagcatcaaa tttacaagca tctttacatg
    33601 ttccaagacc taaacatcca aatttacaca tcttagcacc tgagtttaaa acatttgctg
    33661 ctctacaatc tgatattcct tcatactcat atttatcttt tgcacttgaa caagttcctt
    33721 tacaaataac ttttgctact tgtttctcac ctgcttcagc acttattccc attatttctc
    33781 caacttttgc accaacatct gctccaccta cagggcaacc attaactggt gcattgcctt
    33841 caactatcgc atttgcaaga cctccacaac ctggaaatcc acaacctcca cagttggctc
    33901 ctggaagtac tcctagtata gcttccactc tctcgtctac ttctacagca aactttttag
    33961 atgcaaaatc tagtactatc ccgaagatta agcccataat tcctaaaact aatacagctg
    34021 taagtatcac cattttcacc cctctttttt atagctttat aagtcctgta aatcctaaga
    34081 aagctattga cattaaacct gctgatataa gcgttatagg gaaaccttta aatgcttctg
    34141 gtatatcagc taattctagt ctttctctta tacctgcgaa tattactatt gctaaagtaa
    34201 atccagctcc ggctccaaat ccgtttatta ttgtttctac taaattataa cttttttgaa
    34261 cattaactag agctatacca agaactgcac agtttgtagt tataagaggt aaataaactc
    34321 ctaatgcttg atataaagat ggactcatct tttgaattac catttcaacg aactgaacta
    34381 tagaagctat aaccaatata aaagctattg tttgtaagaa tccagttcca gtttttacta
    34441 ataaaatctg tataaaataa gttataattg aagctagagt taaaacgaac gtaactgcaa
    34501 ctcccatccc tactgctgta tcaaccttct tagaaactcc caagaaagga catataccca
    34561 agaattgaga tgttataacg ttattaacaa gaacgatact taaaaataaa agtattaaat
    34621 tcatttatta cacctccact atgctttttt acttcttaat ttattaaatc ctgcaaataa
    34681 gaatcctaat gttaagaatg cacctggtgg taatataaat attaatactg gttggaaaga
    34741 tgctccaaat aatgtcattc caaataaact accatttcca agtaattctc ttactgcacc
    34801 tattattgta agtgcaacag taaatccaag accttgacct acaccatcaa ctattgaagg
    34861 catagctcct gttttgaaag caaaactttc tgcacgagct aatattaaac agttaacaac
    34921 tattaatggt atatatatac caagagcctt atcaagagca ggaacatatg cttttaaaag
    34981 catacctact attgtaacaa atgtagctat aactactata aatataggta ttcttatctt
    35041 atctggtgta atttttctta ttaaagatat aactaaattt gcacatatta aaactgctgc
    35101 tgttgaaaga cccataccaa ttccatttat tgctgaagtt gtaacggcta gtgttggaca
    35161 catacctata acttgaacaa atgttgggtt ctcatctatt agcccatttt taaatacttt
    35221 agctaaattc ataattaacc ctccttattt attaagagca ctattgaaaa catctatagc
    35281 agcatttact cctgtagtaa ctgctgtaga tgtaatagtt gcacctgata ttgctaatat
    35341 ttgattatct gctgttgtac ttccttttgc tacttcaaga ggctcagcag tttttccttt
    35401 gtattgaccc ttaaatgcat catccttagc ttttgctcca agacctgctg tttctgacat
    35461 attaccaatg tcaacacctg taacttgacc ttcagatgat atacctacca taacttcaat
    35521 ttctccacca tatccacttg gtttagcttt taaagtatat ccaaccactt cagaaccatc
    35581 taaaccttca tacacttcag ctataagacc atctccaagg cctttgtaag tatcttcctt
    35641 catttcttta aattcttttg cttctggaag gactgctttt ctaagttcat tatttgcttg
    35701 tatatttctt tgttctatta ctggagctgt aacctgatta gtagctccaa gagctaatgc
    35761 agctattgca cttatagcaa gtaaagtacc acctaatctc accatactgt tcattattta
    35821 gccacctccc caaatactct atttctaaca aacttatcta taagtggagc aagacagttc
    35881 actaatagta ttgagtaaga tacaccttct ggatatccac catacattct tataacagtt
    35941 gttataagac cagctaatac agcgtatatt atttgacctt ttttagtcat aggcgaagtt
    36001 gtataatccg taagcatgaa gaatccacct aacataagtc ctccagcaaa cagttgatat
    36061 attgcgaaag ttatattaaa tccacctaaa aggaatgtta atataaatac agttgctatg
    36121 taaaatactg gtataacata atttattatg cctttataca ttaggtatac tccacctaat
    36181 aataataata ttgctgatgt ctcacctata catccaccaa tattccctat catcatgtca
    36241 gcaagactaa ttccattgct agaaaggtct gccagtcctg ctgctccatt tttcaaaaag
    36301 cttaatggag ttgctgtagt aactgcatct aaatttttac ctatccagtt aacacctggt
    36361 tgagtccaag cagtcattgc tactgggaat gatgctaata aaaatgctct tgcagcaagt
    36421 gctggattga caaagttaca tccaattcca ccaaatacca ttttaactac taatattgca
    36481 aatattcctc ctactagaca catccaccaa ggaagtgatg ctggtacgtt aaatgctaat
    36541 aataatcctg ttacaacagc tgaaaaatct cctatagtac ttttttgctt cgtaaatttt
    36601 tgatataaaa attcagtacc tactgtacca agtatacaaa aaaacatagc actcaaagca
    36661 ttaagtctaa agaagtatac tcctgctact gctgctggaa ggagtgctat aataacttgt
    36721 ttcattatat atgaagtatc ttcattactt ctcacatgag gagaagatga tactatcaac
    36781 ttattttcca tcgtttttcc cccttaactt tctttatttc tgagcagctt gctttcttct
    36841 ctttgctcct atttctctct ttgctgatct tatagattga agtaatggtc ttcttgctgg
    36901 acaaataaat gagcaagacc cacattctat acaatccatt gctctatgat attcagctgt
    36961 gtcatagtca tcttttaaag aatatgcact tatgaaaagt ggttgtaaga atgatggaca
    37021 tacatctgta catcttccac atcttaaaca gttttgaata tctggagtac gtgattcttc
    37081 ctcatccaaa cataaaattc ctgaagaacc tttgttagtc gctatttcag tagtatattg
    37141 agctattccc atcataggtc cacccattat aaccttacca atcttcttat cttctttaaa
    37201 tccaccacat tgttctatta tttctgaaac taatgttcca acttttgtta ttaggttctt
    37261 tggctctttg atgcaactac ctgttattgt agtgattctt tcaaccaaag gcatacctgt
    37321 tttaatagac ttagctatct gtgctgctgt ggcaacattg tcaacaactg cacctgctgc
    37381 tattggtaat ccaccagatg gaacttcttt gcctgtacac gcataaataa gttgtttctc
    37441 tgctccttgt ggatatttca cttgaaggcc aacaacttcg atttcgctat agtctttagc
    37501 tacattttga attgcttcta tagcatctgg tttattcgtt tcaattccta taaatccttt
    37561 tttgacatct aataccttca ttaatgctct taaaccaaac acaacatctt ctggattttc
    37621 aaccattaat ctgtggtctg cagttaagta tggttcacat tctgcaccat ttaatataac
    37681 tacttctgcc ttgctatctg gtggaggaga tacttttaca tggttaggga atgtagctcc
    37741 acccatacct actattcctg cctcttttat gatacctact atatcttctt tgctcaaatc
    37801 ctctaactga ccttttggtt gaacactctc atgtaattct tccttaaagt cattttcgat
    37861 aactacacat tgagctgaac ctcctggtac ttcatgttgt tcaatagcaa taactttacc
    37921 tgatactgaa gaatgaacgt tacatgaaac aaaaccttgt tgttcaccta ttttttgccc
    37981 aaactttact tcatcaccaa cttcaacaat aggtttagct ggtgccccta tatgttgctg
    38041 aagaggaatg taaactatct ttggagcttg agctttttca agtgcctttg tgttagagta
    38101 ctcttttcta tatggaggat gtatccctcc cttaaaagtt aagagtttca ttgttttacc
    38161 cccttatcta taaaaaaaca ttttttctgc cttctataag tatactatat atattaaaaa
    38221 atcttaacaa ttgttaaaaa aatatccgta tttttataat ttattttata aatcaacacc
    38281 tctatattaa gtcaaattga ggaaaaggat tgcttaatta aacaatcctt ttcatatagc
    38341 tttctaaagt ccaaattttc tgtattttat tttatagaat ttataaacat agagtactcc
    38401 tgcatggatg ataaaagcag acttttactt acactatatt tttcttcatt tgctgctttt
    38461 gaagctgtat caattttttc attcacattc tttacatact ctattaaatt atctttaaat
    38521 aaacgcattt cagaataatc aatcttttca gcttcttttg atatattttg tgatattgcc
    38581 tttctatttg tcaaaatagt attatatttt tccatatcta cattattttc agcattcgac
    38641 caaaaacttg attcttcttt aaagtttgtt attaatttat ttaattcttt agatatactc
    38701 tctatataag cgtagttact tgtatactct aaagacaaca taggtgcatc taagtgtgat
    38761 aaaaatgcat ctggaaacac ctttcttaca ctgcttatct gctttctaac atcattttca
    38821 tcaaaagagg aataggtctg tatttttttt actccatcct tctccataac tgaaaaaggt
    38881 atgtcattat taactaatga tgtctgtatt tttttcaaat catcattatt atctattgca
    38941 gcaacttgaa tagtatacat atcccagcct tctatagtag ctaatttgac ttcttctggc
    39001 tctttagtgt tatttaccgc tttttctgga ttagtttctt gtttatcatc tgaattggtt
    39061 tgtgatttag attttttccc tttttttatt gaatttaatt catcagatat atctttataa
    39121 gttatatctg atgaattatt tagaaatggt attttagccg atacatactc caatatcttg
    39181 ctatctttta tttttgtata agaataatag ccaattagac acaagcaagc aatcgttata
    39241 acatatttac gtaaatcaat cttatgtcta ttattattaa atcctctgta tatcgttttt
    39301 cttctattca ttagtttgtc cctcccaaaa ttatattatt aataaagtat atttttaaga
    39361 gtcacaattt atgaatataa ataattattt cttctaattt tttaataaga aattgtactt
    39421 actaaacaaa tcttcttgca ccaagtaata ctttagacca atatgaacta gttaaattat
    39481 ccactttaac cttattagga ttagtctgag taccactaca atggataaat ttactatttc
    39541 ctacgtatat acctacatga ttagttgttc catcaccatc agtgtcaaaa taaactaaat
    39601 ctcctggtgc ataatttccc atagaaattg cacttccaaa attagcctgt tctctagaaa
    39661 ctctaggaat agaaacgcct acagaatgtt taaaaacata ctgtgtaaat ccagaacagt
    39721 caaatttatc aggtcctgaa gctccccatt gatatggtat tccaataaga gtatatgcaa
    39781 aatctacgat tgttgaacct tttgctgtag atttatctga atttccagta aatgatggct
    39841 ttgagttact attagattga gaattttggt tagatgatga atttggtttt tcttttgtat
    39901 cctcattagt ctcagaaata taactggcac tcacccatcc aattgttcca ctcgataact
    39961 ttaccttata ccaaccatta cttttagctt ttaattcaac aacatctcca ccatttaatt
    40021 ttcctattat agaatagctt gtcccaggac cacttcttac atttaaactc acagcagatg
    40081 ttaccttacc attcttgcct tcaattgatt cttcgctaga tggtttttta tcagaattat
    40141 tagtactatt tgaattacta gaattgttcg ttccaacatc ttctgatgtt ttagaaatat
    40201 attggctact agcccatcca gtaactccat ttgatagttt tattttatac catccattgc
    40261 tttgttctag taactttact acatctccat tatttgcttt accaactaat gaatagttag
    40321 tacctgcacc acttcttaca ttcagtctag aactcacatt tactttacca tttccactaa
    40381 tagttgtccc acttgaagtt ggattatttt gacttgatgt accttcagac gaaccacttg
    40441 ttgatatgta ttttgcactt ccccatccaa caacagaact cgataccctt actttatacc
    40501 atccattaga cttttctaaa atctctacag tttttccctt gtataatttt gctaccttat
    40561 cgcaatcaga acttggtcca cttcttatat tcaaggcact tgctgttact gttccctttt
    40621 caagagcact tgcatttatg gagctaacag atactgctac tgccccaatt cctaaagctg
    40681 ctatagcttt tttcactact atcactcccc ccaacaaatt tttatagtca taaattcata
    40741 acattattat ccatttttag tccctacaat ttaatgataa cttattttct tattaattgt
    40801 aaagaaatta taaacatatc cttaaaatta gtgattttta ggaagtattt caatccaata
    40861 attatctggg tccgctataa aataaattcc catactttca ttttcaaaac atatgcaatc
    40921 taaatcttta tgaagttttt ttgcctcatc aaactcctca acttctaaag ccagatgaaa
    40981 ttcattgtct cctaaattat atggtctatc ccaatctcta agccaagtta attctaatgt
    41041 atgactagta atgccatctc ctaaataaac taatataaaa cttccatctt ctgcttcctt
    41101 gcgctttacc tctttaagcc ctaaagcttt tttataaaaa tctaaacttt tttctaaatt
    41161 agtaacattg aaattgttat gacaaaaatt aaacttcatt ttgaaatcac tctccaattt
    41221 ttattttttt tatattatat cattattata aatttattta aatcatatta agttaaaaat
    41281 ttgaatgatg atgaccaatt accaaatcat ggtaaattat ttaaaagaat atgtcaaaaa
    41341 aagaataagc tctttcaaat gcataaaaaa tttataccca agttatactt aatcatgcaa
    41401 attggcagaa cttattcttt aaaactctaa cttttaagtt tttctttaat aatataattt
    41461 attacttttt ttctaatatc ttcagcatta taatcatctt ctacttctat atccaaaagt
    41521 tttgctattg acctcatatc ttctctagtc tttgacctat agcttgatat taattcatct
    41581 tttattttct ctggttctaa aagatattct tcatctcctc ttttgaaaac aacgtatttt
    41641 tgagcatatg aacttctatt gtagtatata tatcttgata tttttcttaa aatttgatta
    41701 tatgaaagtc gcttgttaat atcaagtttt aagtactctg aaaaagcaaa tagctctttt
    41761 tttgatttaa ataatgcttc tttctttata gttcttgata ttgaagttac actttcttga
    41821 tatgcacact tttttaacat ttttaaatat actctattaa gaccaatact ttgattgaat
    41881 ttttttatat tattattaat aatcttatcc aagttacctt caaagtattt ttttgctcct
    41941 ttttgtgata cataactctc aagttctttg tgggccatat caagtatcat tttttttaaa
    42001 tcctctttat atcccctaga ttccatgata ctccctccct tagctattta tagtatattc
    42061 aaaaagctca agaaatgtga gtcattacaa aattttttta ctttttatgt atatattaat
    42121 ttaaaagggg agataatagt aatgtaaaat caaacatact caatctcccc catttttaga
    42181 cctctattac tcccccaata gaggtctttt tttagtttat caatttctat tcaattccct
    42241 ttagttcaaa tcctaattct gctatcactt cttttaattt ttcaatagat acatcatctt
    42301 tcatatctac agatgcacac ttattttcta aatctacatc taaaacttca ataccatcta
    42361 tatcttctgt taatgcagtt ttaagatggt ttacacaatg accacaactc ataccttcta
    42421 ctaatagttt cttcttcatt taattacctc cttataccct ataagggtat tctatcatat
    42481 agtttatact ttttaaatag aaattaatca tttaatttga ttcattcgat tttatatcta
    42541 aattagatat tccctcatat acatcaaaat catttggagt aattttacct tttaatctac
    42601 tattacttac aatatagttg tttctcacag ctaaacttat aaatgttgca ttatctttta
    42661 tatatgtctg tacatctttg tatgcttttt ttgtttcaat ttcagacgta gaacttgtaa
    42721 gtgcagacat atatccattt aacttatcat cagtatatcc actactcgct ataatactac
    42781 ttgcatctgg tataattgat agtttccatc caactaatgc taaatcataa tttttagaat
    42841 taagagcatt ttccatatcc ttatctgaga gctgatttac tgtactattt attcctatag
    42901 cttttaagtt ttcatttatt atgtttgcag tttttattct ctcactatta tctctgttta
    42961 ctattatctt taagttcatt tcagaaagac ttttctttac ttcctctttt tctctatttt
    43021 ttttctcttc agcttctttt ttgattctat cttcttctgc ttttttaact tttgcttctt
    43081 cttctggcgt aagcttcttt gagttctttt tagctgattt tttctgattt tttactttat
    43141 tgtcattttg atttactgag tttacatttg catattctac cttagcaaga cactctatcg
    43201 ccttatcttt attaaactct aaatccttca tttcactatt atagtatttt gattttgaat
    43261 taagtggaaa attaactgga gtagcatcat ccatataacc ttcactaata attctatctt
    43321 tatctataga gtgtgcaatt gcttttctaa aattaacatc tttgaaaaaa ggattattat
    43381 aattaaataa tacacactca taatctcttc cttgatattt tgttatatta aactcttttt
    43441 cttgaaactt acttaaatca tttaatgtaa cattggttat atcactatca agagccataa
    43501 ccatagatgt ccttgcatct tcattaggca ccatacctac ttttatgttc ttcatggttt
    43561 ttggaacttc ttcatagtaa tctttgttta cagataaaac cattccttct ctttctgtat
    43621 atgattctat tttatatttc ccatttccta tcatattatt attcttgtca tttacatctt
    43681 ttgaatctaa atgattttga gatacaattg gaaaaattaa actatcaatt gaaaaagcat
    43741 atctagcttt aaattttatt gtaaaatctt tatctgattt aatattcaca gaagctatat
    43801 ttttagtaaa ttcattatat ggactatctg cacttttttg aattaaacca atagtatagt
    43861 ttacatcatt tgaagtaaca ttttttccat catgccactt tgcatctttt agctttatat
    43921 ctatactcat tccatcctga gctatattat actccttaac taattgagga attacattgt
    43981 agttctcatc tatagtaaat aaactatcat aaactaaatt cattatataa ccaacagact
    44041 tatcagtatt agttatagga tttatagttt ttggagttac cattgttaag tttatatact
    44101 tagcatctat agaactttga ctttgtaaac ttgaatcact actactttgt tttgttttag
    44161 tattactaca accagctacc ataagagtta tagctaaaac gactgttaag acttttattc
    44221 tcttcattag ttttttcctt tcttgccata tatcttatta taatgttcgt aattattttt
    44281 tactttttct acatattttg aagtttgctt aaaaggtata tcatgtagat tttcgccatc
    44341 tttactatat tcattatttt ctaaccattt tttcacatta cctgaaccac cattatatgc
    44401 agctatcact aaatctaatt taccaaattc tttgtataac ttacttaaat accaacagcc
    44461 aagtcttata ttagtctctg ggtcgaaaat atccacattt tttaaattta gttcctctgc
    44521 tccccaatct ctagttatgt ctaatatttg cattaatcct tttgcttcct tttttgaaac
    44581 agcagaacta ttaaacttac tttcggcttt aataacactg taaactatat tttcatctaa
    44641 attaaattct ttcgaatact tttctacata ctctgaatat tttttaggat ataaaaattt
    44701 atgtattact ttgctttcca ttaatagtgc cccaaataag attataaaaa tagatagaat
    44761 caatactttc ttactattca cgccaattct ccttcatgta ctcaataaat ttatgtgctt
    44821 tactttctaa ttcagttatt gttcccgaat tatctattat ataatcacca tatttggatt
    44881 tttcttcctg actcatctgt gaatttatcc tactaagtgc ttcctgctca ctgcaattat
    44941 ctctcttttg tattctacta atttgtactt cttgtttaca agtaactact aatagcttgt
    45001 caaccatatc taaaaaacca ctttctacca atattgccgc atctaacact actatatttt
    45061 cgcctttttt tctcaatttt tctatttcac ttattatttt ttctcttatt cgtggatgag
    45121 tcaagttatt taattcctta agtttttctt catcactaaa aactattttc ccaagagctt
    45181 ttctatttag agttccatca tcatttttta tactttgccc aaaatggaca aatacttttt
    45241 ccaataacaa cttatcctca aatatcttcc tggatataat atcagcatcc actataggaa
    45301 tgttaagatt tctaaatata tttgataggc tgctttttcc acagcctatt cctcctgtaa
    45361 gtcctaatat caacataaaa tcacctactt tgtctcatac caagaactac ctacatttaa
    45421 atctacatct aagtgaacat ccatatttac agcattttcc atttcttctt ttactatttt
    45481 cttaactata tctatttcat catcaattgc ttcaactatc aactcgtcgt gaacttgtag
    45541 tataagtttt gatttcaaat tgttctcttc caatttttta tgaacattta tcatagcaat
    45601 ttttattata tctgctgcac ttccttgtat tggtgcattc attgcaaatc tctttcctct
    45661 atttctttcc ataaaattgc ttgactttat ttctggtata tatcttcttc tgtttaaaat
    45721 tgttacagca tatccatcct cactagcctt ttctactgtt gtgtccataa actcttttat
    45781 tttatggtat ttagcaaaat aactttcgat atattctttc gcctttggaa caggtatatt
    45841 taaatcatca gctagaccaa agtcactttt accatatata ataccaaagt ttacagcttt
    45901 agctgcacct ctcaattcac tagtaacatc ttccatagga acattaaaaa cttgtgaagc
    45961 tgttttggta tgaatatctt catgatgttt aaatgcttct atcatatttt catcctgact
    46021 catatgtgca agaactctta gctctacttg tgagtaatcg gcatcaacca acttacagtt
    46081 atcatctgca ataaacactt ttcttatttt tctacccatt tccattttta taggtatatt
    46141 ttgaagattt ggttctgtag atgaaattct tcctgtagtc gttatagttt gattaaatga
    46201 tgaatgtatt ctattacttt ctggatttat tatatttaaa agtccttcaa cgtaagtaga
    46261 atttaacttt actatttgtc tatactcagt tatcttatct attattggat gcttatctct
    46321 taatttttct aaaacatctg cattagttga atatccagtt ttagtttttt taattattgg
    46381 aagctctaat ttttcaaata atataacacc caattgtttt ggtgaattta tattgaatgg
    46441 ctctcctgat aattcataaa tttccttttc taatgtagct attatttctt taaattcaac
    46501 actcaattca tccaatatac ttttgtctac ttttatacct tcatactcca tatgacctaa
    46561 gacttctact aaaggcatct ctacatgata aaaaagacca tccatactca tatccattag
    46621 acttctttcc atcataggaa ctgtttcttt aactgtgcat attatttgac ccatatactc
    46681 tgctaaatcc tcaaattcta aatcctcata gtttttagat ttaacaccct ttcctaataa
    46741 ttcttctttt gatttaacct tttttgataa atacttcata gctattgcac tacaatcata
    46801 tgaggtagat gaagttgaat ctattaagta ctcagctatt gttatatcaa aatacatatt
    46861 ttctaagttt atattatatg gttttaaaga tatataatca tcttttaaat tatatccata
    46921 ctttttaatt tcattatttg aaaatatact atctaacttt gacacttcat ttgaatctac
    46981 ataatatatt ttttctccat ctacacttat aaaaatatat attatatttt tttcaagtat
    47041 attaccttgt cctctaacag tttttaaaat aacttgtcct tttttgttta cttcatctat
    47101 aaatttttct atatctttta attttaatat ttctattttt tcttctacgt gttctgtgtc
    47161 atctgaatcg accaaatcaa ctaatcttga taagagactt gtaaatccaa agtatctaaa
    47221 tttatctaaa agttcatctt tatcataatc gccaaaaatc atactatcta aatccacttc
    47281 tattggcaca tctcttataa ttgttgcaag tcttttactt tgaatagcta tttctttgtt
    47341 ttcctctatt ttctttttta cactaccttt aagcttatct gtattctcta ttagattctc
    47401 tatactagaa aactctttta taagctttat acctgttttt tcacctattc caggtactcc
    47461 aggtatatta tcagacttgt ctcccataag accctttaaa tcgataaatt gtgttggagt
    47521 catctcgtat ttttcaatta cagaattaaa gttgtattct tcaacttctc caactccttt
    47581 ttttgttata agagtagttg tattgtctga agcaagttga atagcatctt tgtctccagt
    47641 tactatgtat accttatatc cattttcttc tgctttttta gaaacagtac ctataatatc
    47701 atctgcctca tagccatcta tttccaatct atttattcta aatttatcta aaaggtcttt
    47761 taatggctgt aattgttctg ccagttcatc tggcattccc tttcttccag ccttatactc
    47821 tttatattct aaatgtctaa atgtaggtgc ttttctgtca aatgctacac ttatatgtgt
    47881 tggtttatat atatctatca ttttaaatag cattgttgta aatccatata ttgcattggt
    47941 ctttagccct tctttattat tcatttctgg aagagcataa aaagctctat ttattattga
    48001 atttccatct attataatta attttttttc caaattatat cactcctaaa aattatctat
    48061 ttttgtaaca tatatattat ataccaatat actgttattt aatattccca taataataat
    48121 agtatattaa aattaaatat gttgcaaaca atatattttt ttgctataat aatatacatg
    48181 acatgcccga gtggtggaat tggtagacgc agtggactca aaatccaccg gactaatact
    48241 ccgtgccggt tcgagtccgg cctcgggcac cattaaaaac tcctaaagct atttattcaa
    48301 tagattagga gtttttttat ttttgattta aattttaagt tattttttga aattactgtg
    48361 tctttttaaa atttatataa ttatataaat ttatcttcta gcattaattt aaaaactaat
    48421 ttatttttat ataattttct attttatatc ataacttatt acaacttttt atctaattta
    48481 ttgtatacaa caacactaca aataaattga aataaaataa gcaatccaag aacaactaaa
    48541 acccacttgc ttattaccat tacagaaaat acactcaata tagaaacaat aaatataaga
    48601 attacaagaa cataggacat gatttgatta gtgatacgtc cagctttatc acgtaacata
    48661 attcgtcttt catcattcat ctctatcctt tgatttttta tcttttcttc ataatctact
    48721 gctctttcag gtttactcca atacatatat tgaagaatca tacatacacc tggacctaaa
    48781 gcagctccag ataatcccca aataaaagcc tcaaatgaaa actcagtaaa tgtagccaat
    48841 aaaacaagta aaattccaca aataaaatat ataattccaa catataacat attctttttc
    48901 atttttactc ctcctcataa ataaatattt cttcaatact catatcaaag tatctagcta
    48961 tcttaaaggc taagatgata gaaggattat acctaccatt ttctaatgaa cctatagttt
    49021 gtcttgatac ttgcaaaata gaagcaagtt cttcttgttt tatgcctctt tcttttctta
    49081 tttcttctaa tctatttttc acatctatcc ccctttaata tctcaactac ggaaagtcta
    49141 ctttccataa ttatacaact atattccatt tatgtaaagc ttactttcca tatttacaaa
    49201 aaaataacag actattttca gcctgtaatt ttagaatttc atgttgttta ttaattttaa
    49261 tgttatttat taattccata ttatttatta attttaactt ggtttctgaa ttggaatcca
    49321 aacttctgta gtataatcag gagcattgat atcgccattg ggataaagtt caatatctgg
    49381 taaattagca tacacatacc cagaacttgg taaccactca gtaataattc ttttttgcaa
    49441 ttcctgtatt gccagagcat ttggcattgg agaaacacat tcaaatactg cccatgtgcc
    49501 tgatggtatt aagtattcat gagtatcttc taatacaggc ttatctgttg gagcagcaat
    49561 ataataatct aaacccttac tatttgtaca cacactaact ccaagcaatc cacaaggttc
    49621 attatttatt aactctgaaa gtttatcaat tgttccattt tttatgcatt tagcccacaa
    49681 cttaggaact ttctcaaagc actcttctac aatcatgttc atactttctt ttataccaac
    49741 aatacgaaac cctttctttt ctacaatacg atattgcatt tctatatttc cttttacata
    49801 aatgcttaat gtcatcttag gatatgcttt taaaacaaca ccctttgctc tagctgcaga
    49861 tggagaaatg ttatgtatat tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc
    49921 atacttctca gatagttcta aaattttgat gttactgttc tgaagttcaa atgcagcctt
    49981 agtcatacgt cttcgtctaa tatattcaga taatggtact tctgcaatat atgaaaacat
    50041 tctttgaaaa tgaaaggttg aacaacatgc aatttttgct gcttctgcat aatccactcc
    50101 actttcgaga ttttcttcaa tataagtaat agcttgattt aatttattta tccattccat
    50161 ttgatattac ctccaagcaa attatacagt ttaataaaat aatatacctc tctattcatg
    50221 cacaaaattg taagcattta aatttataag catttttcaa tcaatacaat aaatataaaa
    50281 attgtattat acaataaaaa agttcaataa cattatatta taaaatgtta ttgaactttt
    50341 tataaaaatt aatttaagtg tagttaaatt aagtattaca ctatcttata tattacaata
    50401 tcctactatc ttatagtctt ctggcacttt ataatctttg tctggagtac ctaaattcca
    50461 ttttaaatca aacatagttg cacttattat tgttgcaaaa taaagcataa ctataccaat
    50521 gtctattttt tcttcatata aattagtatg accatctttt ctaacagcta aaactacagt
    50581 gcctccatca actataaatc tccatggttg tctatttaat gtagatggag ctaatcttgc
    50641 atagtgaaat gcatcaagaa gtgctctttc ttctaagatt tctatacttg caacatttcc
    50701 ccattcgttc atataaacaa catcttctac acctagtcta gtagacccct tatctgttac
    50761 aacttgcata tcagattttg aataattttg cccagttttt gtagcattta atactttagc
    50821 attacttaca ttttctccat atccaatagc tataattcct gttacctctt tatctgaatt
    50881 aattccaagt ttttctttta ccttttttcc atcttcaaaa gtaacccaac aagaaccaat
    50941 tccaagttct gttactttaa agattatact ttctcctatg taccctgaat tttcaatgta
    51001 attatctttt atgtcagata atattataat ataacttgga gaatctatca tatttccttt
    51061 gtaccctgct actttatcta aattattaaa aacttcactt ctattcataa ttcttacatc
    51121 aacatctatt tctggaacta gtttcttaca agattctgag tatttcttaa tttcactaag
    51181 atattcttct ttaatatctg tgttcttata ttctctaaca gactttctat ttaatatgat
    51241 tgttttataa tccataattt tatcctcccc aaactagtat tgtcttaatt atatacccat
    51301 taatatatta ttttactcat ttatttaata aataaagatt acaattttgt aattattatt
    51361 tattaataat attccacttt tacattaata atgaccagtt gtgcaaaatc cattgccaac
    51421 caccaaataa aaacatatta taaaggtaat ataaaataac aaacaaatct taacaaaaaa
    51481 ttaaactttt tttacacgaa agaaggcttc ccgttgcctt ctttattttt ataaaaaaat
    51541 aagctgtttc taaaatgatt tcacattttt gaaacagcca agttattcta taatgacaac
    51601 tataatttga ggatgtgagg gagagttgtc attataaatt tagggtaatt atattatgtt
    51661 ttaagctagt tctttctata tttatataat acattttata tatgaatttt atatgagatt
    51721 ggtataaatg atatatgaaa ttacatatgt ttatattata ttaaatttta ttattacatt
    51781 ttccacaaca atactcatat gtctccacat tatttggaat atttacaaat acaaattctt
    51841 tacagttctc acaatacttt aatatagata tagttccaca agtgtaacat tcaaatatta
    51901 cacaatcctc attttcctct actatttcaa actttttact atgttcacca cactctgggc
    51961 acttgtgatt atttacattc ataaatactc ctccttatta gtatagttac atgtcaaaaa
    52021 cttaattaat gtattctata ttacttatta tatattctat aataaatcat attctaatat
    52081 aataaaacaa attaaataaa aaaaaaccat aaattttaaa tttatggatt aattcttgta
    52141 atgataactc tatttgagga tgtgagggag ggttatcatt acaatagggt attatattat
    52201 ggtttttaag ctagtctttc tacacctcat atgatacata tgctatatga attttatatg
    52261 atttttacat aaatatttta tgaaatttaa attttttagt cattttgaaa tttgtaaaaa
    52321 gttctctaaa aatccacagc atcataaaca gccaaaattt aagtagaaca tcataaaaaa
    52381 atttgtccaa taattttaac aagtaatctc acctcattat aattattgac acaaacttaa
    52441 ataaacaatc tataaatctt ctataaatct taaactttcc ccctattgat acacaatttc
    52501 tagtttgtca attttatttt gcatttctaa gaaaatttct tcaacagaat tgttaaatat
    52561 attgcctaaa tagattcttt tatctttttg aaatgaataa gattttacac taccatcaga
    52621 agaaatatat aaatgactcc actctgctcc atattttgaa aacctctcct ttgtatcttt
    52681 aaacctcata tcttctattt ttattgcttt ttctctgtct cctcttacta cagtgtgacc
    52741 cctttttatt aaactggtta tttttatatc ttcttttaat ttaatacgtg tatcatcatt
    52801 ttttgaatat actttccata tcactttatg acgataatta tctaattcct ttattaagct
    52861 tctatcaata gtaaaactat ctttagttac cacaattaaa tcaaaatggt ctaacatata
    52921 ttgaaatatt tctctcatac ttttattttt taaaatatcc tcaccaataa cctctataaa
    52981 tctacagcct ttctcataca tgcagttcaa taaacttgtg atttcagaaa aataatcatt
    53041 tgtattatta tctccatcta tttctatagt aaagtacaaa ggagtaatgt atgatttatt
    53101 tcctgttatc tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg ttaagtatac
    53161 attctcataa aacataaaaa attccataac tttttctttc atattatgga tatttctata
    53221 tctattggat aatatattta ttatatcgtc agtagtattt ttcccatcac attcagccag
    53281 tataacaatt tgcttccaac ttaagctatc cacatttccg atactttttt tatatccatc
    53341 ttcttcatca taaataggac caattataat gttttctttt ggccacagat gaaccctagt
    53401 tccatctttt aatttcggat atttattttt tacagactca taaaatctac ttgtatcacc
    53461 catgattctt tactaccccc catagtttaa atattctatg ctaaaaatag cactttattt
    53521 ttattaattc aacaattatt caaaattggt cattatatat gtttaaatgg atttaacatc
    53581 aaccttattt atatgttatt gatgtttata tatgttatat aaaatcttat ttctcatact
    53641 tcaatgtaat cttaactatc tctggtttac aattaagcct aattaaaact tttgagcctc
    53701 ctaagccttt atttaaaatc atagtgctat tatctttttc atattttccc cagtcatact
    53761 taggaaagaa ctctctattt ggagataata cacctccaac aaaaggtatt cttattatac
    53821 ctccatgaac atgtcctgct aaaaccaaat ctgctcccca ttttgcatat ttttcaaaga
    53881 aaaatggtgt atgtgctaaa agaatgttat attcattttc attaacttta cctagattgt
    53941 cttctacaaa attgtcactt agttctaatt ttttgctatt agcaaataaa tatggatagc
    54001 attccaatgg tataattaaa ccatataaat taagatggct attacctcta tatattctta
    54061 cagattcatt ttccatattt attattttct ttgaatatag ttccttaaaa taatccttat
    54121 atagatgttt atactttttt attaaagatt tttgttcatg atttccaatt atatgatata
    54181 cttcatattt ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa tttttatttt
    54241 ctccatctac taaatctcct gttataaata taacatctgg gtttaatgat tcaattttat
    54301 ctatcaatac cttattattt ttatcaaaca ccttattatg taaatcactc acttgaacta
    54361 ttttaaagtt atcaaattcc tttggaattt ttttgttgaa aatagtatat tctgtcgttt
    54421 ctatatgctg agtttcatac ttatacagaa tatacaataa tattaatact aatactgtca
    54481 ttgaaacctt tataatcata ttctcttcct ctttattcta ttttgttaat attattataa
    54541 tgcttttatg ttttttttaa aacaataaca tcatcacata catataaatc ttttcgacat
    54601 tataaaaata agttatcctt ttctaaaatt ttcatttatt ttagaaaagg ataactataa
    54661 aattatctat aaacctctat attgtgaaat atagtttttc ctttatttaa atttttatat
    54721 atatgctcta aagttccatc aaacctaata gaatgtccag actctaaaat ataagttttc
    54781 ccatccacat taaggtcaag tttaccttct attacgagta catactcttc taaaacatga
    54841 ctgtgtgtag atgaaacatg agtacagttt tcctcaagct caattataaa aagttcaaac
    54901 cttctttgag ggctaaaagg gtatattgga tataacttca tgttcccttt ttgctcaaca
    54961 ataggctcta cttcttcttt ctttataacc tcataggtat ttgtattttc atttaatagt
    55021 gtagtaaaat ttattttaaa tccagatgaa attttccata aagtagatac tgtcggactt
    55081 gattcacctc tctcaatctg tccaagcatt gctttactga cacctgtcat ctttgaagct
    55141 ttgtctaaac ttatgcccat ttcttgtctc aataacctta aattttctcc cacattagga
    55201 aatttattat ccatgttaaa atcctcctga tagctatttg tattctatga tttttactcc
    55261 tgtaagttct actatctctt ttaattttaa catatgattg ttacaaattt catcaaattc
    55321 atatatataa tcttcaagcc ctaatgcctt atatttatcg attccaaatt tatgatatgg
    55381 aagtaactcc atcttgcctc ctggaacatt ttgtttgaca aataatgcag tatttcttat
    55441 attttcttca ctatcattta ctccataaat aattggcact cttataacca tagatttatt
    55501 caactttgaa agcctacata tattatctaa tataatttta ttacttactc ctgtatattc
    55561 tttatgaata ttatcatcca tagattttat atctacaaat atgtggtcta ttttctcaaa
    55621 tacatcattt accttattcc aatcaaaata tccacaagtt tcaattgcaa tatttatgcc
    55681 tttatcataa aaaatatcaa ctaattctct taaaaaatca atctgaagtg taggttcacc
    55741 tccagaaaaa gttatccctc cattagattc aaagaaaaat ataaaatctt tttcaacttc
    55801 ctctactatt tcttcaacac tcatttcttc ggtcattata caaatggcat ctttaagaca
    55861 taccttgata cactctccac ataaatcaca tttattattt atttgcgctc tatcaaatag
    55921 tgaacttata ttttgaggac aaacatctat acatctatta caggatacac atttatcttt
    55981 cataacacct aattttacta tgttactcca ggaatctgga ttagaacacc acttacattt
    56041 taatttacat ccttctaaaa atatagtagt ccttattcca tctccatcat ttactgaaaa
    56101 atgttgtact tttacaactc tacctttttt actcataatc tacctcataa ttaaaaattg
    56161 ctatgtgaat ttctagctat tatagcatct tgcatttctc ttgataaatt aacaaattga
    56221 gtactatatc cagcaactct cactaataaa tctttatatt cttctggatt ttcttgagct
    56281 tttaatagag tatcatttga tatcgtatta aattgtacat ggaatcctcc taaagcaaaa
    56341 tatgatttaa tcatagatgt tagatttcta agtcctcttt cagtttccac caaatcttga
    56401 ttcagtctta aatttagaag tgttccacca gtatgaacat catgatttat ttttgatgca
    56461 gatttcattg ctgctagtgg acttgttgta tcagtaccaa caaatggaga aactccctct
    56521 gtcaaaggtt ttgtcgcttt tcttccacaa ggagttgcgc ctacgatttt tccagttggt
    56581 acatagtttg atatacccat aaatgcacta ttaaatttag agccaaaaat atccttgtat
    56641 tttctagttt cagtataata aaaatcagaa acttcaattg ctaatgaatc tacataatca
    56701 ttatcatttc catacttagg aacatccaac gcaagttttc ttaattcttc ataaccttcc
    56761 caatcattat ttagtgcttt tgttaattct tctaacgtag tcaccttatc ctcaaatact
    56821 aattttttta tagctgccaa tgaatttgaa actacaccta accctatacc agttaataca
    56881 ggacctatat tatattttgc tcctccttta cttaaatcaa ctcccttatc caaacatcca
    56941 tctacacaag tagataaaaa tggtcttggt accatttctt tatgaatttg ttgagcaaca
    57001 gtagttccta taacagaact ttttacaaga tatgctaatt gctttaaaaa ctcatcctta
    57061 agttcttcaa aagaagtaaa ttctgtgata ttttttgtat ctaaccccat tttttctcca
    57121 gttaatctac tcttaccttc attcatagca tattctaaag ctgctgcaaa attgatattt
    57181 acggctgaag tccactgtcc tgtttttcta aaatgaggaa ctacacaacc acagttactc
    57241 caatctctag catcttctgc atcatatcca tcttgtaata acatttgtgc ccctgcacta
    57301 tcgctgtgta tagcagggaa accagttcct tgttttacca atttagcaac tgccattacg
    57361 aaattgtctg gtgctccttg actcactcta acacttaatc ctggttggtg agtttttaca
    57421 ctttctgtag cctttaaaca catatatgag atatcattcg ttccatcagt accatttctc
    57481 tttttaccac caacagtaag attttggaat tgattatatc ctgcaaagta atttgctgta
    57541 tttgctgata tagtccatac ccattctgat aacttaatcc aaagtgcttc tatcaattct
    57601 tgagcttctg attcagttat tttaccttct ctagcatcat tttcataata tggatacata
    57661 tattgatcaa atctacctaa atttaatgcc aatggatttt ctgatagaat accacctaat
    57721 tgaacaaacc atacaaattg tattgcttca taaaaattag ttggagggtt tgctggtaca
    57781 ttcatacata tctctgaaat ttttataagt tctaactttc tcttttcatc tgtttcttgc
    57841 ttactcatct cttttgcaag atttgaatat ctttgtgcca atgtcataat accctgtgca
    57901 actatagaaa cagatttgta aaaatctatc ttttcaatat tttctgaaac tgatatatct
    57961 aattctttaa tttttttatc tgcatcttcc ttaatccctt tatatccttt tacaaacaat
    58021 atatcttgat aatctggtgt tacttcacca actgcctgtc tccacttaga atcattatct
    58081 attattcctg tatctactaa tatcttagct gtatcttctg ggattcttgc taagaaaact
    58141 tcttccaaag attttccctt ccaatatgga aatatttctt ttcttataaa ttctatttgt
    58201 tcttttgata ttacatatgg gtcttgagtt cttttatcaa aagtatccat ttctttgtct
    58261 acccattgcc aagaaaactc tggtgtcaat atccctgttc ttctaaattt tcctgctgta
    58321 cctactatta attcattttc aaagattttt ataggaagtt ctttacatgt ctccaaaaaa
    58381 gctttagctc ttcttataca gatagcttct ccctcagttt ttttatgtga ttctgtaaaa
    58441 attctagccc tttcacaaca tatcataggt ttagaattaa tatattcttg tctaaatgct
    58501 tctattctat tagtttgact catttaaaat ccccctttgg ataatatata ctacattttt
    58561 gtatgttata acatacttta attatattat actgtccttt agcataaaat ggaagcaaaa
    58621 taacttattt tttttcatct accttgaaca cctatatatt aatacttttt ttgttaaatt
    58681 tataaaatct aaaaaaattt tctatgatat attttctata ttgttatagt tatgtctaat
    58741 atactaatga tttgaaatat caaatttgta tactagactt agttttaaaa ccttagttga
    58801 ttgtttaact ttgttaactt caacatgtat atccttcata taaggaagct ctacaacacc
    58861 tactccctta ttaaagtctt cttcaaccat agaaaaaatt tctttctcag ttaagttcgt
    58921 atcagtcaat gatttaaata aattgtatat aaatttaaca tatttatcac tctcagataa
    58981 cttatcatct tctggatact ctttagcaca gagaatatct attgttactt ttccttcttt
    59041 atcgtcttca gacatagaat agaataaact tcgaatattt ttttcaggtg gtttttcagc
    59101 accatttata gatactacaa agctaccatc tgattttgag ttagtaatac tataaccact
    59161 gtctattata gctttcgaaa atctttgaga taaatctttt tttacttctt ctctaccttt
    59221 cgcatcatta atcttttgtg agtaattatt aaatagctca atagattcat cttttttatc
    59281 ttcaacttgt tctttcaatg aatctttact aatttgttct gagctactta agttcttatc
    59341 agatacagga gacatacacc ctaccatcaa tgctaagcta agatttaaag ccacaattat
    59401 tgccactcta tttatattcc tttttacttt catacacaat cctcccatca cactaaccgc
    59461 tctaaagtat tattcattgc actagaatat atttatattt tctttatatt aaagatttat
    59521 tattcaatac atatcacctt cctatccaat tcttataact tactattagt aaaattataa
    59581 gctccttaat acagaaataa ggatgttatc ttttgttatt aaaagataac atccttattt
    59641 ttttgtatca atacatatgt cccaactggt aacttaatcg ccccaactgt cagtatcttg
    59701 gtccaatggt atatatatat ttagtataaa tttatcgata ctattttcaa ataataattc
    59761 tccattatat ttttgtaaag aagatttaat actctgaata cctattccat gtacgaactt
    59821 atccattttg tttgttaaca atatattttt attaaaagta agctgattta gtttactatt
    59881 ttcacattta agtacaaaaa atgaccttgt tatagttcct ttaattctaa tatatttatt
    59941 aatattttca tccactattt tatcacatgc ttctattgca ttatccaaaa tattagaaaa
    60001 tatgcttgac acatctatag gttttacaaa atttactttt gaaaaattta tagagcaagt
    60061 aaatttaatt ccttttttta aacatacttc actcttttcg ttgattataa tgtctaatat
    60121 catatttcct gtattataga tatatttaaa tgtttttatt tcatctttta aattgttaac
    60181 atattcatta atttctttgc tattgtttct aaggttgtct atacaataaa tgtgattatt
    60241 tatatcgtga tatagtttct taactcgcat atgagattct tgaatactta aataatgcgc
    60301 atattgcata tctaacttat tttttattaa ttcagcttga gattttaatc tattctcttt
    60361 tattatttct ttaaacaata gtataagaaa tatactacta gctcctacca attttggatt
    60421 aacaaaatta tcaaaatagt atccttctga atgtaatgac ccaaaccgtt ctaatttttt
    60481 taatacataa tttacaagca tattcattgt attaactaat aaggcaaata ataaatatac
    60541 ataatttctc ttatcttggc taattaattt tatagacttt ctaaaaaagc taaatataag
    60601 atatataaga ataattatta aattactata tatctgaatt tttatagatt ctaagtaagg
    60661 tactaataaa gattctttag atgaaataac attacctgta attgcaaaaa atagtattct
    60721 atatataaca gcatccaaac atttataata tacactaaat agataaaaga aaatggaata
    60781 cttaatataa cccatcttat aaaaataatt atagtatcct ccccaagcaa taaatgctaa
    60841 tatataaaat aaatagtata tattactaaa gtaatttcct ccattaaata atctacttat
    60901 ctgaattata atataaataa gaaacaagac aatatagaat actatttttt catttttttt
    60961 actatccttt ttataaatta aaattccgtt cattatagaa aactgtataa atatagataa
    61021 aactactaat agctcagtca taaagtttag catattgtat caccaagaaa ttttaagaat
    61081 ttctccttaa cttctttata tctatatcta ctaataggta cctcttctcc actttccagt
    61141 attgcagtat taggctttat attttcaaca tatcttaaat ttactataaa acttttatgg
    61201 catcttataa atttatatgg atttaattct ttttctattt tgcctaaact atattttata
    61261 tcaaaattct tgtttattgt gtgtattagc atatcttttt tttgaacttc tatatatttt
    61321 atttcattta aataaatctt atatgtatta gacttatttt ttattgtaat atgactttcc
    61381 ttatttattt caatatcttt gatacaagtt agcacatgtt tttttagttc ttccaactct
    61441 attggcttta gtaaatacct ataagctctt acctcataac cttcttgcac ataatctatt
    61501 aatgaagtta caaatattat ttccatttca ttgtctactt ctctaatctt tcttgcagta
    61561 tccattccat ttaatttttc catttttata tcaagaagta atatatctat atcttttaaa
    61621 ttactttcca gtaactcttc tccagatata tacttttgta tctcatattc tatacttata
    61681 tcttttaata tattctcaag gtaatcttct attaaaagtt gtgtttcttt ttcatcttca
    61741 caaactgcta tcttaatcat aaaatgcctc cttacataat ttctagttaa tacaattaaa
    61801 atatttataa tatatttaaa gataattttg tatatctgat actttaggat aacattttta
    61861 aattattttt atcaatactc aatcccatac aactattatt tgatatttca atttaaagac
    61921 agaaattact ataattaaaa acagtactta ctgacaaaag aagatgtctt aaaatattaa
    61981 aaaaataatt ttaggacacc ttcttttatt tatttaaact ttttaagcct taaataatac
    62041 attaatctcc tatataattg tactgcatat aaaatcaaca atataatctg tagtacaata
    62101 gcagttacag atgctccata accactatat aaatttctta tttcaatatt caataaatat
    62161 ataaatatta ctactaacat aaagaatata aacatattca taaataaaat tgactttact
    62221 ttatttgttt tatcagaatc actatataat ttaggaggta tgtctttgta ttctttcgac
    62281 catatatacc atgcagatgt acccataaaa ctaccaatac tcctaaatac aagattccag
    62341 ccatttgatt tatgttcatc aaaataatta ttctctacat catagtagta atcaacagtg
    62401 tattttattt ttcttggact tcccttatga aaataaaata ttcctccaaa tttatttatc
    62461 ttatataaat tatatccttt agcttcaata tcttctaacc atttctctat cttatcaggt
    62521 gaactatacc agtcaaattt gaattttatt ttcttttcca tagcactcat tctccaaaat
    62581 gatattttta aatagcttaa aaatctattt aaataattat atcattttga tactaatttt
    62641 aggttacaat catatattta aaagttattt aaatagtaat aaactaatag ccataacacc
    62701 cattcctgca atcaatccat aaatagataa atgatgttcc ccgtactctc tagctgatgg
    62761 caataattca tctagtgata tgaataccat aattcctcct acaattgcaa atacaattcc
    62821 aagagttata tcatttaaaa aatttctaag aagtacatat ccaataatcg ctcctaaagg
    62881 ctcagacata ccagataaaa aggaataata aaaagctttc tttttatctc ctgtggcata
    62941 ataaactggt acagatacag atataccttc tggtatattg tgtattgcaa ttgcaattgc
    63001 tatcggaata gctattgtaa catcatcaag agctgatata aatgtagcta atccttcagg
    63061 aaaattgtga attgctatag ccattgctgt aaatatacct gttcttaaaa gagacttatt
    63121 ttttttgata ttcttttctt ctatattctc cattttcttt atttcatgtg gattttcttc
    63181 ttgtggaatt agcttatcaa taacagcaat gatagccata cctataaaaa aagctattac
    63241 agttgaccaa cttcctaatt tctctcccat agattttgtc aaagcatctt gtgctttagg
    63301 aaaaatttct atcatagaaa cataaatcat aacaccagca gaaaatccta agcttataga
    63361 aagaaactta gtatttgttt ttttagcaaa aaatgctata caacttccta taccagtaga
    63421 taaaccagcc aataatgtta tcaaaaatgc aaatattaca ttattcaaat aaatcacctt
    63481 ctttttaatt gatattgatt atcattttct ttatgctatc atatatattt tatatagtca
    63541 atatttataa aaaatataca caataaaaaa gcagagatat aaacttagtc tctgcttttt
    63601 ctttatagta tattaaaatt atttatttaa gaaatccact gcatattgca catataaagc
    63661 agttccaatt tctagagcat cttcatctat attaaagcat ccatgatgat gaggataaca
    63721 agcacctttt gactcatttc tagctcctac aaatgctagt gcaccaggtg ctatattcat
    63781 aaactcagct aaatcttctg ctcctgttac tttttcaaat agtgttatac agtcttctcc
    63841 aaatagctta attgctgcat ctgtagctat ctttgaacat tctttgtcat ttataactgc
    63901 tggtgttaag tacccatact ctaattcagc atctgctcta tatgcttctg ctgtagattt
    63961 tgctatacgt tctaaaattc caggaatttg ctttctaagc tctggattaa atagccttat
    64021 tgtaccttct aagatagctt cactcgcaat tacattaaat ctagttccag aatttaaaac
    64081 tccaacactc acaactaatg gttccagagg acttacttct ctactaacca tagattgtag
    64141 attcataact attgctgaac ttgcaagcac tgcatcaaca ccttgatgag gaagtgaacc
    64201 atgaccacct ctacctttta cagtaatttt aaagaaatca gcagaagcca ttcttggtcc
    64261 ttcttctacc gatatggtcc cagattctac atcagtccaa agatgtattc caaacacact
    64321 atctacacct tccatagcac catcttgaat catagctctt gcacctttac caacttcttc
    64381 tcctggctga aaaaataatt ttacagttcc attgatacta tccttaatat catttaacac
    64441 ttttgctgcc cctagaagca ttgatgtgtg accatcatga ccacaagcat gcataagtcc
    64501 ttcattttta gatttgtatt ctacatcagt gcattcaaca acttgtagag catccatatc
    64561 acccctaagt gctacagtct ttcctggatt atctcctttt atagttgcaa ttacacctgt
    64621 acctccagct gatacatatg gtatccctat tttatcaagt tcttctttaa ctctctttga
    64681 tgttctaact tcttccatac ttttctcagg attttcatga aattcccttc taagcttaat
    64741 tacatagtct ttataactct ttgttatttc tttaatatcc attctatacc tccaagtagt
    64801 acacattctt cttatttaaa atacttttta tcaattaaat actatattag atttacaaag
    64861 atacctgcta ttactacaga agctattgta actgtagtaa atcctccaac aagcatcttt
    64921 ggaagcattt tatccattaa aaattctttt tcttctggtg tttcagccaa agcctttact
    64981 gcctcttcag ttaatatata gtttggtgga aaaccataaa gtgatgtaag cgaaactgaa
    65041 aaagccattt ctttactttc acctaaaagc ttacctacta taatagagaa aattcccata
    65101 cctattacac caaatattat gattcctaca agaggaactg ccatttcaac aagcattgct
    65161 ggtgtagctt ttgctaaccc tgcaaatata aaagccatca atgatgtcat taaccatcca
    65221 aatgaaccag atttattaag tggttttcta tcaactattc caagttctga tgctattaca
    65281 ccaaatatta aacatataac atatcttgat acaacttcat ttgtaagatt tgtaaattgt
    65341 actgctaacc atgctacaaa tcctaatcta agtattatca tatatgtcgt atcatatttt
    65401 tctggtaagt ctggtattat cttaaatctg cttttttgtt caggtaaatc cttaactttt
    65461 tcttcatctg tatttacagt tacttttcca cttctaaagt ctgataaaag tctttttcct
    65521 tcttttttaa gcattaatgc tgtaatagga taacccgcaa atccttgtgc aacatacatc
    65581 aaaattgcaa gtactgataa acttgcaaga ccttttgctg tagcagcatc tgccatcatt
    65641 atagatgcaa ctagccctcc agtaagagga ggtgtagcta ttactgctgt atcccatcca
    65701 aatactactg tccctaatgc caaagcacct acacatattc ctactattcc tgccaaggct
    65761 attactattg ttttccattg acctgctaat tccttaatac tcatcatagt tcccatatga
    65821 gttattaaca aaagcatacc taattgagct aatggtgttc ctaaagcggc taagtccaca
    65881 atattttctg ggaaaaaagt ccaaaatcct actataaata taattgctga tacaaaaacc
    65941 gacggaacaa aagctttagt ttttgctgaa actatatctc ctatcgtcag tgatactaaa
    66001 actagacaaa aagctgttat tgcctccata caaaatcccc cctcataata aatatatttt
    66061 tataaaatgt atgtatagtt atccattatt ttactacttt tgaaaaacat ttcaagtcaa
    66121 aattcgattt ttttcataaa attcgataaa ttccataaat taaagttagt tttctaaatt
    66181 attctttttg taaaaacgtt atcccgacca ttggctattt aactctattc ttacaaagag
    66241 aattaaatgt aatttgcaaa aaataaaaca taggatacat tttattcata aaacatctta
    66301 taaatataaa atgaattgtc tttatgaatc ttaatatatc ctatgtttgt aagtttaatg
    66361 tgtatgttag ctatggtgta aaatttgtat actagttatt gtgtaaccta ctatatactc
    66421 catttttgtt tactagctct tcatgagtac ctctttcttc aattccttta tccgtcaata
    66481 ctatgatttc atcagcattt tttatagtag tcaatctatg tgcaacaact aaatttgttc
    66541 tatctttact taattcctct aaagactctt gaatttctct ttctgttaca ttgtctaaag
    66601 cagatgtagc ctcatccaat attattattg gaggattctt taaaaagatt cttgaaattg
    66661 aaattctttg tttttgccca cctgataatt ttacgcctct ttctcctata taagtatcat
    66721 atccatctgg caaagtctct acaaagtcat gtattctagc ttttttagca gcagctatca
    66781 tttcttcatc tgtagcatta gggttcccac aaagaatatt atctcttata gttcctgtaa
    66841 ataaaaatac atcttgttga actataccta tattttttct caatgatttt aaactaacat
    66901 cttttatact ctttccatca atttttatat cacccttatc aaattcataa aatcttggca
    66961 atagattaca taatgtagtc tttcctcctc cagaaggtcc aactaatgct atagtcttac
    67021 cagcttttat agataaactt aaattatcca aaacttgagt cttatcttca tatgtgaaag
    67081 atatattttc tatctcaatg tcacctttta cattttcaag ttcaatagga tttttagctt
    67141 ctttttgatg gtcttgttcc attatctcta tgaatctttt aaaacctgtc ataccatttt
    67201 gatattgctc tgtaaagttt attaattttt tcataggttg tataaacatt tttacatata
    67261 gtatataagc agcaaaatca cccaaagtaa tctttcctat ataagtaaaa taaccaccag
    67321 ctattacaac tacccactct aagatatcca tgtataaatt cataccagag taatattctg
    67381 ccattacctt ataagaatct tgtcttgctc ttctaaataa tttattgctt cttacaaact
    67441 tatttaactc ttctttctca gtacaaaaag acttagttac cttcatacct gctatactat
    67501 tttctaaagt agcatttaca tcacctatag taactctact cttcataaaa gcaatattca
    67561 tcttatcttt tttagctatt gcaaaccaaa ttataaacgg taatatagca aatattataa
    67621 gcgtaagagg aatatttata tctataagta taaagaatga accaaccaac attactatag
    67681 aaatgaataa atcttcaggc ccatgatgtg caagttctgt tatatccatt aaatcattta
    67741 ctattctaga catagttact ccagatttgt tattatcaaa atacgtattt ggaagtcttt
    67801 gtaagtgtgt aaatacctca tttctcatat cagcttgcat atctacacca ataacatgtc
    67861 cccaatactg cataaaatag ttaagccctg cttttgctat aaatataagt ataagtgcta
    67921 ctgcaaaaac tactaacatt cttaagtttt tatttggaac aacgtcatcc attatatttc
    67981 tagttatcat aggataaact aaatcacata aggaaaatag aaaagctgct ataaggtcta
    68041 gtgtaaaaat tttcttatat ggcttataat atttgacaaa ttgttttatc ataagtatta
    68101 aatccctcct ttattagtac ttctaaaaaa tacccataaa ttagtataac ataacaataa
    68161 ctttaaatct tttataaact tacaaagtaa taacaatctt tttcataaaa ttgaagattt
    68221 atatcatata gttgtatact tgaaacaaca taaaaagagc tatatctcta tatttaaaat
    68281 tttataaaga tacaactcta tatttaatac taaatttttt ttataaatac aaaactactt
    68341 caatgcattt aatatctttt tatctaatgc tgttgtattt atacctgtta tatatcctgt
    68401 tgcctttatt gtaggtatat cataagttgt tggtagtata gtagtagata cgattaaatc
    68461 agcaccttct tgttttgttg aacattcagc aattttacat tgctcaactt ctgcttgtat
    68521 attattttct ttaaccaatc tttctactct gtcacaaact attgttgatg ttgcgattcc
    68581 tgctccacag gctactaata tttttttcat atattgtcct cctacaattt ctttaatatt
    68641 ttataactca aaataaaatt gataaatttc ttatatcttt atttatataa ataattatat
    68701 cattttatat aagaagaaca tcttaaaatt attcttttaa tatgtttttt atttcaacat
    68761 tgcttatact taaaatttca ttttttatac aagactctat gtactttgat atatttttat
    68821 aatcatttaa attaaactgt tcaatattca atttatattc tatatcactg acaataccta
    68881 tcaaatcaga cttattagat aaaatatcct catacttacc ttttctagtt acttccaatc
    68941 ttttaaatct ataattttta tatccttcta tgataatcaa gtccatatct tcatataatt
    69001 gaatcatttt atataatgga ttcttctctt ttagttcttc aatatatgct actcgtttat
    69061 ctgatattat acagactttt ctcgccccag attttctatg tttataagta tcttttcctt
    69121 cctcatccat atcaaaatca tgacaggtat gtttaataac tccaacacga tacccttttt
    69181 tatttaattc ttttattatg ccttcaatca atgtagtttt acctactcct gaatatgaga
    69241 caactgacaa tatattatac ataaaattgt gtcctttcat aaaactatct aataacaaca
    69301 tccactaatt gattttcttt tattgtctca ttctttttaa tcttaatcat acaattactt
    69361 cttagagttg cagataatct agcttttgca tctttcttat catttagctc tacatacaat
    69421 ttaccttcac ttgtatatag ttttccactt acaaatctat ctttatttga aagtttatta
    69481 aattctcctt taaatatagc ttttatatga tgtgttccct tcttatatgt taaatactca
    69541 ataacaggaa ctatcacatt atcaaatacc actattgctg catgtggatt accagaaaga
    69601 ccaaataata gcttttctcc caatgtacct acataacaag ctccaccagg tttaatgttt
    69661 atcttccaaa ataattctgt tgcccctatt tttttgaata catctggaat taaatcttta
    69721 tctccaacag aaacaccacc tgtggtaatt aaaaaatcta cactctgtaa catattcttt
    69781 accttttttt ctaactccaa tatatcatca gaacaattgc atattgtatc tgcttgtatt
    69841 ccaagtttta ataatcttga atataaggta tatctattac tatcgtatat tttaccattc
    69901 tctaaagtct catttatatc ttgtagttct gtacctgtat tcaaaattcc aacttttaaa
    69961 tcatcataaa cagttacttt ggatattcca agacttgcta atactccaat accatcatat
    70021 cctatattct gatttttatt aataatcaat tgcccctttt ttatatcctc acccttaaat
    70081 atgtaattat cgtattcctt taattttata tttaaagtta tcatatcact tataactgtt
    70141 acatcttctt tttttattac acaatttgca ccctgtggta tctttccacc agtcattatc
    70201 ttcactgctt catttttatt taatacccta ttaaatacat ctcctgcata caaagtccct
    70261 ataaccttta aatttctatt ttcagaattt gcattatatg cataaccatc atagggtgac
    70321 ctatcaaatg gaggactatc aattgttgaa tatatatctt ctgcaactac cttatttact
    70381 gcatctataa gatttactat cttgaccctg tttattggta aaacattttt ttcaataatt
    70441 tttatggcat cttctaattc tatcatttta aatactcccc aatatatttt ttatttaaga
    70501 tatttctatg taatatatta tagattataa aagttggaaa tacttttatc ttatatttaa
    70561 ttataactaa tagttatcta taagtgcagt tcaaatttta taaatctaat aaaaaaactg
    70621 ttcccagcta gaaatttcct tctatcttga aacagcatca tatgctttag aatattttat
    70681 tctaataaag ttccttttat tatttttaca attttatcac tttcaatagt ttttatagat
    70741 tttattttgt tatccacaat acttattgta agtacctctt tttttaatat tttgttatct
    70801 tctttcaatc tataactctt aactacttta ctgccttcat gattatatgc tgttaaatca
    70861 taattcgcat ctcctacaat ttcatttatt ctggaatctt caacaccaac atactctccc
    70921 aggtcaatat gtctaaaatt ttctagtgat acagccgatt tttcatggtc acttttttca
    70981 atcactatac tagattcagc atcaatgact gatatacctt gtgatgagaa agaattggtt
    71041 tcaacattga caactttatt ttcgttaata tcaacatata aagcacttcc atctagttca
    71101 tcatttccag tatattttgg ataaactaat gtcatataac ttgatgtatt tatatcttgc
    71161 attgtatttg attcttttaa aactttaggt tttatataat aaaatgtatt ataaggtata
    71221 cccatattct ttataatgta cttataatct ttattcataa tttcattaac atcattttga
    71281 acttttgtca tagttttttg ctgtgttgct ttacttgcta cttgattttt ctctttagta
    71341 ttttctttat gacatccaac aagtataata cctaaaatag ttacacaaac taatgacctt
    71401 tttaacttca taaatcaatc ctcctttctt aaattttata gtatttatct atatctcaat
    71461 ttacagttat ctcttttttc aataattata tttattatat tataaaataa ttatataata
    71521 tttatttgaa tttaataaga ttaagaagct aattgtaatt tttctgtaat aaaaaacact
    71581 ttaaagttga attttgaaaa ttcaatttac gatattttta ttgtaagtac cttacaacat
    71641 tataccaaat tattcctaaa caatcaaaaa ctccatgaat atttaaaggt tttctatttt
    71701 aaaaatcacc tttgactatt aatttatcaa atatgttata atagttttag ataatttaag
    71761 gaggattaaa taatggattt taaacaactt gaagtatttg ttgctgttgc aaaacatcag
    71821 agtttttcaa aggctgcaag agaacttttt ttaactcaac ctactgtaag cgcacacata
    71881 cagaatttag agagagagct agaaactgtt cttataaata gaagcaacaa agttattaca
    71941 ctcacaaaat ctggtgaaat tctttatgaa catgctatat atatattgaa taattgcaaa
    72001 cgtgcaattt atgatataaa agaatattca ggaaaaatag aaggtattat agatatagca
    72061 tgtagttcca tacctgaaac ttatatttta cctgatttta tgaagagttt ttctatgagt
    72121 tatccagatg taaaattttc cataagccat tatgattctc aatatgctat atcagaaatt
    72181 ttaaatgaac gaataagctt tggattagtt ggttctaaaa tcaataatcc tcaaatagag
    72241 tacctagact tattagatga tgaacttgtt cttatcactc cttcagattt taaaattgat
    72301 aataaaaata attgtatcga tataggagaa ttggcttatc taaattttat tatgagaaaa
    72361 gaagggtcag gtacaagaaa cttaatacta aacacactta gtaaaaataa ctttccagta
    72421 agtaaattaa atgtaatcgc tcatgtagaa tcaaatgagg ctattaagga aatggttaga
    72481 ttaggtcttg gagtgtcctt tatatcgtac atttcagcta tcgattatct caatgcaggt
    72541 aaaataaaat gttataaaat aaaggatgtt gattttacta gaaagttttt cttcatttac
    72601 tctaaaaaga aaactttttc cccattagaa gataaatttc taaacagatt atgtgagtat
    72661 ttcgaaatta taatataata actttaataa agtagtgctt aattttgagt ttataactaa
    72721 ttaataagct aattataact atcacataaa tgcttataaa ttattatgaa ccatctttct
    72781 tttttattta atttagtcaa aaaatccctc gttcaaatga acttgggatt tttttattta
    72841 tactactcca atagcagcca atactataca aataatcata actgaaatta atatcgtagt
    72901 tgtctttacc ttttttccta ataaccaata cataataccg aatatcatag ctggaagcat
    72961 acatggcatt atttggtcta agtaagtttg aataggctct gcccattctc cactacctac
    73021 attaacacta agttttaaca ttacattaga tgcagtcata cctccaatta ccataagccc
    73081 taacattgaa gctgcttcca taacttttga aactatactt gaaccactca catctttaaa
    73141 gaattgagtt ccatacttaa atcctttatc tagacatata tagcgtataa taaaatgagg
    73201 aacatttatt attaaaagga ataaaatcgg tcccattatg ttaccctgag aagctaaagc
    73261 tataccaacc ccagctgcta taagctttaa tgttccccag aaaaatgaat ctcctatccc
    73321 agaaactgga cccattaaac ttgatttaac agcatttata ctattagcat caaattcttt
    73381 attctttgca ttttcttctt ccatagctcc agatatacct accaaaagag tacttatatg
    73441 aggagtaact gacataaatt ctaaatgtct ttttaaagcc ttagatttct tttctttatc
    73501 atctttatat aatctacgga ttattggtgc catcatgtat gcatatgcta tattttgcat
    73561 acgttcgtag ttccaagatg aatctaatgt caatgacctt ctaaaacatt tccaaaagtc
    73621 tttcctcgtt attttactat ctggtgaaat tgtctctaat ttcttagaac tcgttgtcat
    73681 ctactattac ctccttctct cctacaaagt taacatatat taaagctatt gctaatccaa
    73741 acaatgcaac tcctgttact gttatgttta ggtaagcaac caatataaat cctaataata
    73801 aaaatgctac tgttttctta ttaagcatca ttctagctaa catggcaaaa cctattgctg
    73861 gaatcacgcc tgctgcaaca tccattccat gagttacata tgatggtata aatgaaagta
    73921 ttttttctat tactggaaca cctaatgaaa atcctatacc tactattatt gctaatatta
    73981 atttatttcc tataccaaat aataattgca cacgttctac gccttcaata ttcccctctt
    74041 ctgcataacg gtcagcaata tgagctcccc atgtaagtac tactgtcatt aagaagttat
    74101 ctataagcaa tactagagtt gctataggaa ctgcaagtac aactgctgct gatacatctt
    74161 gacccgttaa tattacaaac gctgtggcaa taatagaacc agacacaaaa tcaggaggat
    74221 tacttgcccc aacaggaaat gcacccataa atactaattc taatgttgaa cctattacta
    74281 tacctgtatg aagattacct agtgctaaac caacaagtgg tgcaacaact ataggtctat
    74341 tatgcattgt agaacctaca aaactatgca tatatgcaaa gaatgcaatt agagttatca
    74401 aaataacttg tgtaaattcc attttcatcc ccctaaaatt atattaattt cattacattt
    74461 gtagcatcat catcaggaac taattgtaca aatacattta cgccttctga atctaactct
    74521 ttaatcatct ttatatcatc ttttgataca tgcactgctt tagatatatt ttcacgatta
    74581 tcatctgatt ttgttccacc aaggtttata gatttaatag cttttacctc tttagcaagt
    74641 ctgtaaacat cttctacaga ttcacaaagt atcaataagt tgtatttatc agtaacacct
    74701 gagttaagtg cttttataga atctgctatg cttttcatta ctaacttaac attagatggt
    74761 ttagccattt taagtcctgc cattcttaat tcatctttta ataagtcatc acttgctatc
    74821 aatatacagt ctgtacttaa aaactttgtc catgtaaatg ctacttgtcc atgtataagt
    74881 ctgtggtcta ctcttactaa tttaatcatt ataattcctc cctaattttt ttgatttcat
    74941 ccatgaatgt ttttacgtgt tctacactaa catccccaaa atctttgtaa ttatctttaa
    75001 agtaacttcc tattattgcc atatctccta atttaagctg tttttttgca ttttctaatg
    75061 taactccagc agctataacc aatgggaagt ctcctaaatt tttacgaaac agttctattt
    75121 tttccattga tgtttcttga cctgtagcat tttctgtaac tgctatagca tcacatcttg
    75181 tcattcctat ttttaaatct tcttcaacat cattttctga taatactggc tgatatttaa
    75241 aacgtacgcc tccaattaaa tacgctggac attttgaacg ttgtaattta aaaaactctt
    75301 ctaaagtagc ttcatctcta ggtttaacat gacctactac agaatcaacc tgtatataac
    75361 tagcattata ttttgttgcc agttcaaatc ccatagtatc aacatttaaa cagtttactc
    75421 catatggtat tgataaatta gcttttgata catattcaag tattctttct aaatcataat
    75481 aattaccata gtaattttct aacattattc catctacacc attttcttca aagattgtaa
    75541 tctctttttt agctctttca aagatatctt ctggagtatc tccttttaag tgtatcattg
    75601 ctataattgg ttttttactt ttaaatactg ataatattcc cttttttttc ataaactaaa
    75661 atacctcttc ttcttcttta atttgtaatg tcacgctttg tattcctctt ctagattctt
    75721 ccactaattg gtctatatct tcaactgtta attcatcaga aaaccttgta agaagttcta
    75781 ataccatatt caaattcatt ccagaaaata acctaacatt atgtagatta gttaatctta
    75841 acattgctgt acatacactt gctccatata agtcagataa aattatatat tcacattctt
    75901 tatcttctga tattgttttt tctaattcta aagcaaaatc tgaagcactt tgtccagggt
    75961 acaaaccata cgaactgaca caatctgcta aatctcctac tatcattttt actgcattta
    76021 aaagcccttt tgaaaagtct ccatgagatg ctaatattat ctttttcata ctcatcctcc
    76081 tactaaatta tagattatat ttctgctgat actgcatatt tgtacttatc acttcttata
    76141 aattgctcat tgtaatataa gacatcatta ttttctgtat acaccgattg tcttactttc
    76201 aatagtggca tattacttgc acatttaaga tggtcagaat attctttgtt tgaatgtttt
    76261 acttcaaata tattttctac tcgtttaggt aatgtgctta tattattaga tataacctcc
    76321 attaaagatt tatcttctaa ttcttctttt aataaaaaca tgtaatctat tctaaaatag
    76381 tcaacttcaa aaataacagg tacttcatct attaatctaa gtcttttaat acatattacc
    76441 ttctcttcta gttcaatact taaagcttca gctacttgct tatctgcttt ttttattaaa
    76501 acagagatta ttttggtact tggttttgca ttaatacgat gacaagaatt agtaaaacta
    76561 tttcctgcac atgttgcttc tattctctct ggtattgtta cataagtccc tttaccacgt
    76621 tttttacata atatatcttc ttttacaagt tcattaagtg catttctcac agaaattctt
    76681 atattttatt tatgtttatt tatatttatt tatgttttta tgagtacatt tatttacaac
    76741 ctatctaaaa caatttcttt attctatttt atttatattt ctttatttgt attttatttt
    76801 attaaactgt catggtggca aaaaaggtgg caaaaataat ttgccaccta aaataatctt
    76861 tatttattct ttctctttta aatttttaaa atgttcttcg agttcaatta atttatcttc
    76921 atccacttca tcatctttac tatgtaacgc tgatattaaa cttgaaattg aattcttatg
    76981 tatgttcttc aaaaattcct ttgtttcaaa tcttagatat tttttctctt ttaccattgc
    77041 attatagtgt gtaagtttac ctattttctc tgtacttaaa aactcccttc gctctaacct
    77101 ttttaaaact gtgaatgtag ttgattcatt ccattcgtat ttttcttcca ttgcagcaac
    77161 taattcccta gaagttaata ccttgttagc ttcccaaata tatctcataa cttttaattc
    77221 cgcttctggt aatttactaa ttttcatagt ctacacccct ttaccgattt atgtatatgt
    77281 taaattctat atcaataaag ttttacaaac aagagaatta aggtaaataa ttccatccaa
    77341 tttttaccat atcattcttt taagttttta tagtactctt ctaatgagtc taacttctct
    77401 ttacttacat ttttatgcgt agtggtaagt gaacgcatta tttttctact tttactagat
    77461 tttagtacct ttttcttaaa agcataatat tcttcattat ctattattat tttataataa
    77521 gattgaaatt ttataatatc cctagccaaa taacctttgt caattagcct ttttagtagt
    77581 atttttattg tacttttatg ccatccataa atctgtttta tttcttttac aatttctttt
    77641 ttaggcatta gagtgtcctt tttccataaa tactccatta ctattaattc tgctcttaat
    77701 aatttttcta ttaatatttc ttctttcaat ttatgtcact tccttaaatt cgtctaactt
    77761 aaattaataa taggagcttt ttaagacaaa aactagaggt aagttttacc aaatattcta
    77821 catatttagt ctgttttttt aagtgtaaaa taatgttcat atctataaat ttatttaact
    77881 ttcaaacaat cttgttgaat aataactaat tctattgtat tattttagtt gtagaataaa
    77941 actaaatcgg caaaactaga gaaatttagt ggcataaata tatatgaact aagaattgta
    78001 aaatgagtta taagttatat catccagtta tcaaaaacat atactctttt tgtttttgtg
    78061 aaagtttttt aggtggctaa taatatatat acttagagat aaaataaaat atatttctaa
    78121 tattcatagt tttatttttt ctcaaaatgc gatataataa agatgtaaat tcgtatcaaa
    78181 aagagataag agaactgcaa ttctcctacc tctttagaaa taaggaacta taatctattg
    78241 tcgagagtgt agtttccgaa aaaatttagt actttttata tttaattttt actattagtt
    78301 tattaatagt taaattaaat ataaagccac tcttagcttc cgactttgag tggcttttta
    78361 ctttgtataa atcaagtaaa aattttgcta tgtaataaac tgctactatt tccacacttc
    78421 ccacctcctt tcattagaaa gtaggttaga gcataatgtg gaaactgcca ctcttagata
    78481 tttagttcct tacatataaa ttataacata attt
//
LOCUS       DAYAOY010000004        77163 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYAOY010000004 DAYAOY010000000
VERSION     DAYAOY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.004"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(72..845)
                     /locus_tag="ACGI6B_000308"
     CDS             complement(72..845)
                     /locus_tag="ACGI6B_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HFG9749781.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(845..1252)
                     /locus_tag="ACGI6B_000309"
     CDS             complement(845..1252)
                     /locus_tag="ACGI6B_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9749782.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1256..1573)
                     /locus_tag="ACGI6B_000310"
     CDS             complement(1256..1573)
                     /locus_tag="ACGI6B_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HFG9749783.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1585..1845)
                     /locus_tag="ACGI6B_000311"
     CDS             complement(1585..1845)
                     /locus_tag="ACGI6B_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFG9749784.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1845..2141)
                     /locus_tag="ACGI6B_000312"
     CDS             complement(1845..2141)
                     /locus_tag="ACGI6B_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFG9749785.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="ACGI6B_000313"
     CDS             complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="ACGI6B_000313"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFG9749786.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="ACGI6B_000314"
     CDS             complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="ACGI6B_000314"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFG9749787.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6558..7673)
                     /gene="recF"
                     /locus_tag="ACGI6B_000315"
     CDS             complement(6558..7673)
                     /gene="recF"
                     /locus_tag="ACGI6B_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFG9749788.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="ACGI6B_000316"
     CDS             complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="ACGI6B_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFG9749789.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8034..9140)
                     /gene="dnaN"
                     /locus_tag="ACGI6B_000317"
     CDS             complement(8034..9140)
                     /gene="dnaN"
                     /locus_tag="ACGI6B_000317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFG9749790.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9382..10701)
                     /gene="dnaA"
                     /locus_tag="ACGI6B_000318"
     CDS             complement(9382..10701)
                     /gene="dnaA"
                     /locus_tag="ACGI6B_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFG9749791.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11360..11497
                     /gene="rpmH"
                     /locus_tag="ACGI6B_000319"
     CDS             11360..11497
                     /gene="rpmH"
                     /locus_tag="ACGI6B_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFG9749792.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11575..11919
                     /gene="rnpA"
                     /locus_tag="ACGI6B_000320"
     CDS             11575..11919
                     /gene="rnpA"
                     /locus_tag="ACGI6B_000320"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFG9749793.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11951..12202
                     /gene="yidD"
                     /locus_tag="ACGI6B_000321"
     CDS             11951..12202
                     /gene="yidD"
                     /locus_tag="ACGI6B_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFG9749794.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12234..12941
                     /locus_tag="ACGI6B_000322"
     CDS             12234..12941
                     /locus_tag="ACGI6B_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFG9749795.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12910..13539
                     /gene="jag"
                     /locus_tag="ACGI6B_000323"
     CDS             12910..13539
                     /gene="jag"
                     /locus_tag="ACGI6B_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFG9749796.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13651..15030
                     /gene="mnmE"
                     /locus_tag="ACGI6B_000324"
     CDS             13651..15030
                     /gene="mnmE"
                     /locus_tag="ACGI6B_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFG9749797.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15125..17020
                     /gene="mnmG"
                     /locus_tag="ACGI6B_000325"
     CDS             15125..17020
                     /gene="mnmG"
                     /locus_tag="ACGI6B_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFG9749798.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17013..17732
                     /gene="rsmG"
                     /locus_tag="ACGI6B_000326"
     CDS             17013..17732
                     /gene="rsmG"
                     /locus_tag="ACGI6B_000326"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFG9749799.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17895..18680
                     /gene="noc"
                     /locus_tag="ACGI6B_000327"
     CDS             17895..18680
                     /gene="noc"
                     /locus_tag="ACGI6B_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HFG9749800.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18815..19588
                     /locus_tag="ACGI6B_000328"
     CDS             18815..19588
                     /locus_tag="ACGI6B_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9749801.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19590..20453
                     /locus_tag="ACGI6B_000329"
     CDS             19590..20453
                     /locus_tag="ACGI6B_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFG9749802.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20486..21628
                     /locus_tag="ACGI6B_000330"
     CDS             20486..21628
                     /locus_tag="ACGI6B_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9749803.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21762..22337)
                     /locus_tag="ACGI6B_000331"
     CDS             complement(21762..22337)
                     /locus_tag="ACGI6B_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HFG9749804.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22597..23889
                     /gene="rstA"
                     /locus_tag="ACGI6B_000332"
     CDS             22597..23889
                     /gene="rstA"
                     /locus_tag="ACGI6B_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HFG9749805.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            23994..24596
                     /gene="yedF"
                     /locus_tag="ACGI6B_000333"
     CDS             23994..24596
                     /gene="yedF"
                     /locus_tag="ACGI6B_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFG9749806.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24669..24932
                     /locus_tag="ACGI6B_000334"
     CDS             24669..24932
                     /locus_tag="ACGI6B_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFG9749807.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24947..25132
                     /locus_tag="ACGI6B_000335"
     CDS             24947..25132
                     /locus_tag="ACGI6B_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFG9749808.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25235..26431
                     /locus_tag="ACGI6B_000336"
     CDS             25235..26431
                     /locus_tag="ACGI6B_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9749809.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26645..26923
                     /gene="rpsF"
                     /locus_tag="ACGI6B_000337"
     CDS             26645..26923
                     /gene="rpsF"
                     /locus_tag="ACGI6B_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFG9749810.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26960..27394
                     /locus_tag="ACGI6B_000338"
     CDS             26960..27394
                     /locus_tag="ACGI6B_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9749811.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27413..27640
                     /gene="rpsR"
                     /locus_tag="ACGI6B_000339"
     CDS             27413..27640
                     /gene="rpsR"
                     /locus_tag="ACGI6B_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFG9749812.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27783..28103
                     /locus_tag="ACGI6B_000340"
     CDS             27783..28103
                     /locus_tag="ACGI6B_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFG9749813.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28111..29067
                     /locus_tag="ACGI6B_000341"
     CDS             28111..29067
                     /locus_tag="ACGI6B_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HFG9749814.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29078..31072
                     /locus_tag="ACGI6B_000342"
     CDS             29078..31072
                     /locus_tag="ACGI6B_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFG9749815.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31073..31522
                     /gene="rplI"
                     /locus_tag="ACGI6B_000343"
     CDS             31073..31522
                     /gene="rplI"
                     /locus_tag="ACGI6B_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFG9749816.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31541..32869
                     /gene="dnaB"
                     /locus_tag="ACGI6B_000344"
     CDS             31541..32869
                     /gene="dnaB"
                     /locus_tag="ACGI6B_000344"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9749817.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33074..33382
                     /locus_tag="ACGI6B_000345"
     CDS             33074..33382
                     /locus_tag="ACGI6B_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749818.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33464..34753
                     /locus_tag="ACGI6B_000346"
     CDS             33464..34753
                     /locus_tag="ACGI6B_000346"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFG9749819.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35079..35154
                     /locus_tag="ACGI6B_000347"
     tRNA            35079..35154
                     /locus_tag="ACGI6B_000347"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35112..35114,aa:Lys,seq:ttt)
     gene            35161..35235
                     /locus_tag="ACGI6B_000348"
     tRNA            35161..35235
                     /locus_tag="ACGI6B_000348"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35194..35196,aa:Glu,seq:ttc)
     gene            35240..35315
                     /locus_tag="ACGI6B_000349"
     tRNA            35240..35315
                     /locus_tag="ACGI6B_000349"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35273..35275,aa:Val,seq:tac)
     gene            35349..35423
                     /locus_tag="ACGI6B_000350"
     tRNA            35349..35423
                     /locus_tag="ACGI6B_000350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35381..35383,aa:Thr,seq:tgt)
     gene            complement(35806..36798)
                     /locus_tag="ACGI6B_000351"
     CDS             complement(35806..36798)
                     /locus_tag="ACGI6B_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9749820.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36807..37865)
                     /locus_tag="ACGI6B_000352"
     CDS             complement(36807..37865)
                     /locus_tag="ACGI6B_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFG9749821.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            37990..39453
                     /locus_tag="ACGI6B_000353"
     CDS             37990..39453
                     /locus_tag="ACGI6B_000353"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFG9749822.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39555..40352
                     /locus_tag="ACGI6B_000354"
     CDS             39555..40352
                     /locus_tag="ACGI6B_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9749823.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40365..42467
                     /locus_tag="ACGI6B_000355"
     CDS             40365..42467
                     /locus_tag="ACGI6B_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9749824.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42535..43014
                     /gene="rlmH"
                     /locus_tag="ACGI6B_000356"
     CDS             42535..43014
                     /gene="rlmH"
                     /locus_tag="ACGI6B_000356"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFG9749825.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43163..43474
                     /locus_tag="ACGI6B_000357"
     CDS             43163..43474
                     /locus_tag="ACGI6B_000357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9749826.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43522..43839
                     /locus_tag="ACGI6B_000358"
     CDS             43522..43839
                     /locus_tag="ACGI6B_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9749827.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44052..44753
                     /locus_tag="ACGI6B_000359"
     CDS             44052..44753
                     /locus_tag="ACGI6B_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9749828.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            44977..46278
                     /locus_tag="ACGI6B_000360"
     CDS             44977..46278
                     /locus_tag="ACGI6B_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9749829.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46415..47509
                     /locus_tag="ACGI6B_000361"
     CDS             46415..47509
                     /locus_tag="ACGI6B_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFG9749830.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47511..48644
                     /locus_tag="ACGI6B_000362"
     CDS             47511..48644
                     /locus_tag="ACGI6B_000362"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFG9749831.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48662..49771
                     /locus_tag="ACGI6B_000363"
     CDS             48662..49771
                     /locus_tag="ACGI6B_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFG9749832.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49836..50576
                     /locus_tag="ACGI6B_000364"
     CDS             49836..50576
                     /locus_tag="ACGI6B_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFG9749833.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50581..51573
                     /locus_tag="ACGI6B_000365"
     CDS             50581..51573
                     /locus_tag="ACGI6B_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFG9749834.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51761..52642)
                     /locus_tag="ACGI6B_000366"
     CDS             complement(51761..52642)
                     /locus_tag="ACGI6B_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9749835.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52915..54303
                     /locus_tag="ACGI6B_000367"
     CDS             52915..54303
                     /locus_tag="ACGI6B_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HFG9749836.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54331..55284
                     /locus_tag="ACGI6B_000368"
     CDS             54331..55284
                     /locus_tag="ACGI6B_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9749837.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55344..55568
                     /locus_tag="ACGI6B_000369"
     CDS             55344..55568
                     /locus_tag="ACGI6B_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749838.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55561..56073
                     /locus_tag="ACGI6B_000370"
     CDS             55561..56073
                     /locus_tag="ACGI6B_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFG9749839.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56105..56959
                     /locus_tag="ACGI6B_000371"
     CDS             56105..56959
                     /locus_tag="ACGI6B_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HFG9749840.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57349..57477
                     /locus_tag="ACGI6B_000372"
     CDS             57349..57477
                     /locus_tag="ACGI6B_000372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749841.1"
                     /translation="MDYEIKRVIPKVVNTMLTLILQSLGDFVIVNRNTFNEHKEFI"
     gene            57493..58287
                     /locus_tag="ACGI6B_000373"
     CDS             57493..58287
                     /locus_tag="ACGI6B_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9749842.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58391..59197
                     /locus_tag="ACGI6B_000374"
     CDS             58391..59197
                     /locus_tag="ACGI6B_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFG9749843.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISNI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59259..59780
                     /locus_tag="ACGI6B_000375"
     CDS             59259..59780
                     /locus_tag="ACGI6B_000375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFG9749844.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60195..62282
                     /locus_tag="ACGI6B_000376"
     CDS             60195..62282
                     /locus_tag="ACGI6B_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9749845.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62297..62584
                     /locus_tag="ACGI6B_000377"
     CDS             62297..62584
                     /locus_tag="ACGI6B_000377"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9749846.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62611..63882
                     /locus_tag="ACGI6B_000378"
     CDS             62611..63882
                     /locus_tag="ACGI6B_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFG9749847.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63940..64539
                     /locus_tag="ACGI6B_000379"
     CDS             63940..64539
                     /locus_tag="ACGI6B_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9749848.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            64544..65449
                     /gene="dapA"
                     /locus_tag="ACGI6B_000380"
     CDS             64544..65449
                     /gene="dapA"
                     /locus_tag="ACGI6B_000380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9749849.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65555..66187
                     /locus_tag="ACGI6B_000381"
     CDS             65555..66187
                     /locus_tag="ACGI6B_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HFG9749850.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            66664..69171
                     /locus_tag="ACGI6B_000382"
     CDS             66664..69171
                     /locus_tag="ACGI6B_000382"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9749851.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69513..71675
                     /locus_tag="ACGI6B_000383"
     CDS             69513..71675
                     /locus_tag="ACGI6B_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFG9749852.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71680..72297
                     /locus_tag="ACGI6B_000384"
     CDS             71680..72297
                     /locus_tag="ACGI6B_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9749853.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            72486..72830
                     /locus_tag="ACGI6B_000385"
     CDS             72486..72830
                     /locus_tag="ACGI6B_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFG9749854.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73400..74266
                     /locus_tag="ACGI6B_000386"
     CDS             73400..74266
                     /locus_tag="ACGI6B_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9749855.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74379..74783
                     /locus_tag="ACGI6B_000387"
     CDS             74379..74783
                     /locus_tag="ACGI6B_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HFG9749856.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            74947..75396
                     /locus_tag="ACGI6B_000388"
     CDS             74947..75396
                     /locus_tag="ACGI6B_000388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9749857.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75552..75929)
                     /locus_tag="ACGI6B_000389"
     CDS             complement(75552..75929)
                     /locus_tag="ACGI6B_000389"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9749858.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76200..77108
                     /locus_tag="ACGI6B_000390"
     CDS             76200..77108
                     /locus_tag="ACGI6B_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HFG9749859.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        29528 a         9128 c        12867 g        25640 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata ccatctttaa aacagtaggt caatactttt
       61 tttatatttt tttataaatt tttttcatat tcttttttca gcttctctac tacctttttc
      121 tctagtctag aaacagtcat ctgtgatatc cctagttctt tagctatact agattgtgtt
      181 ttatcaaaga aaaatctatc tttaaaaatt ttaacttcta attcatttaa agtttcaata
      241 aacttattaa taaaatcttc atactctata cttccaaagt taccttcttc ttttccaatc
      301 ttatcaataa gagttatatc tttatcctct gaatcatcat tacttgatga atccaaagac
      361 attggttgat atccataaga agcctccata gcctctaaaa catcttcttc actaacgcct
      421 atataatcag ctatatcttt tacctttgga tgcttcttat tttcttgttc taatttaatc
      481 ttcgcatcac ttatcttttt gcttaattcc tgtattcttc ttgggactct aagtgtccat
      541 accttatctc taaaatactt ttttatttct cctactatag ttggtgttgc aaagctggag
      601 aactcaaatc ctttctcaac atcatatcta tcaatagcat aaattaatgc caatgaagca
      661 acttgatata tatcttcaaa atcaacacct ttgtttatat acttcttagc taacaatctt
      721 gctaaataca gatgtctttc aataagaata tttctaatgt ctacattttt atttttatta
      781 tataaattaa atagctcttt tgtatccata tttaggtaat gtgtagcatt agctacattt
      841 ttcattaaat atcaactcct aaatatttag tcatttttat ttttgtccct tgattatgtt
      901 ctgattcaat atctacatca tccattaaag ttttaattat aaacaatcca agaccactct
      961 cttttggatt ctctaaatct ggtgtactta ttgagctaac atcatatcct tttccattat
     1021 cttgaatttc tatattaatt gcattatcta aaatattaaa tactatatta aacacatcat
     1081 ctttactgtg ttttattgca tttgtacatg cttcagatac tgctaccttt atatcttcta
     1141 tatcatctat tggaaaaccc atcttattgg caattccaga tgctgttaat cttattatac
     1201 taacataatc tggatttgta gtaatttcca tctttatagt ctcacaagcc aaagtttatc
     1261 cctccacttt aaatatttta tctagacctg ttatagtaaa aatctttctt acgttactct
     1321 taggatttac tatgtatatc tctttttcat ttatttttaa tttttttaat acacctatca
     1381 tagctcctaa gcctgttgag tctatataat ctaaatcttt aagattaatc tttacatcta
     1441 acatattttt ttctattaaa gcatgtaaat gttcctttag tttatctgct gtagaaacat
     1501 caagttctcc atcaagacaa acattccaaa atttattttg tgaatctaaa tttgaatcaa
     1561 tattcataga catgtaaaaa cctcctattt ccttttaata attttaaata tatttcctat
     1621 actcaatatt ttattaatta cataaactat atcatctatt ccctttgata ttgatgatgc
     1681 attttcaaca atatcttgta tatgtctttc attataatct acaatcttat atgccttttc
     1741 aacaacctta tttatgctgt ttagagtttt agctaaataa attgctacta taagagcaga
     1801 gcttcctact aacactgctc caatttgcca cccccatgca ttcattagtt aacatctccc
     1861 tcatcatcaa agcttttact tattactata tcttcttctg aaatctttat atcttcttca
     1921 ttattatcat catccactaa atttattatt ctctctttaa catcattaaa tgtttcatgt
     1981 agaacttctt ttggattttc tttgacatca ttaaacttct cttttgtttc ttttcttagt
     2041 tcagaacctt ttttaggtgc aaaaaacata ccaaatataa accctaaaat agctcctaat
     2101 aataaaaatc cacctttttt acaacttctt ttattgcaca taacaattac tcctttcaat
     2161 tttaaatttt tatctaacta cattatacaa aaagaatagg atatttatcc tattcttctt
     2221 caatatttat tttggcaatc gaaactacat tgacttcatc attcgtcttc attaatttaa
     2281 caccacttgt aactcttcca aacagtgata tttcattgac tcttattcta ataagaactc
     2341 catcagaatt tataatcatt atctcatcat cttcgttgac catatctgca cctacaattg
     2401 taccagtttt ttcagatata ttataagttt taattccttt accagctctt atttgacttc
     2461 tatactcttc tatatttgtt ctctttccaa aaccattctt acttacaact aacacatctg
     2521 tacctttact gcaaaggttc atagacacaa caaaatcttc tttacttaaa gttatacctt
     2581 ttacacccat tgctgttcta cccatatatc ttatatcatt ttcatcaaat cttatagaca
     2641 taccttcttt agttactaaa agtacttctt gttttccatc tgtaagttct actccaataa
     2701 gctcatcatc atctcttaaa cctattgcaa taagaccaga tttatttata tttttgaact
     2761 cttctctctt agtcttttta acaatacctt ttttcgttgc aagtaataaa tattcatttt
     2821 catcattacc atcaataggt attagagtag caattttctc atctgctgac aattgaagta
     2881 aattcactat agcagtacct ttagcctgtc tcttaccttc tggtatttca tatgcattta
     2941 atttaaatac tctaccttta tttgtaaaga ataatagtct actatgagta gttgtagtta
     3001 ccaagtgcct tacaaaatct tcttctctag ttgtaagtgc tgaaatacct cttccacccc
     3061 ttttttgact cttataagta tcagatggaa gcctttttat atatccaaag tgagtaagag
     3121 ttattgctat ttcttcatca cttataagat ctctcatatc tatttcgcct tcagcatgtc
     3181 ttatttctgt tcttctctca tcagagtaat tttcttttat tattgtaatt tcatccttta
     3241 ttacatttaa aagtaatctt tcatcagcta aaatctcttt taatctattt atcttcttga
     3301 ttaaatcttc atattcagct tctatcttat ctctttctaa acctgtaagt ctttgaagtc
     3361 tcatatctaa tatagcttgt gcttggattt cagttaattt gaatttttct attaaaccta
     3421 gcttagcttc ttgcccagtc tttgaagctc ttatcaagct tataacagca tctatattat
     3481 ctaaagcaat ctttaatcct tctaaaatat gtgctcttgc ttcagcttta tttagttcaa
     3541 atttagttct tcttgtaaca acatcttctt gatgtttaat ataatgatat aatatttgtt
     3601 taagatttaa aactcttggc tgaccatcta caagtgcaag cattattata ctaaaagtat
     3661 cttccatttg agaatgttta tacaaattat ttaatactat attagcatta gcatccctct
     3721 ttaattctat aacaattctc ataccatttc tattactttc gtctcttagg tctgatatac
     3781 cttctattct cttttcttta actaactctg ctattctttc aaccagttta gccttattta
     3841 cttgataagg tatttctgta actattattt gctgttttcc ttttggtagc tcttctataa
     3901 aagctctaga cctaacttta acttttcctc ttccagttct gtatgcttct gctatacttt
     3961 cttttcccat tataattgca gctgtaggga aatctggtcc ttgaacaaat tttattaaat
     4021 catctacact acactctgga ttatctatca aatatacagt tgcatcaatt acttctgcta
     4081 aattatgtgg aggtattgaa gttgccatac caacagctat accatttgag ccatttacta
     4141 ataaattagg atatctagct ggcaatactg aaggctcttt taacgactca tcaaagtttg
     4201 gtttaaagtc tacagtttcc ttttcaatat ctctcagtag ttctaatgat aatttactca
     4261 ttttagcttc tgtataacgc atagcagctg gtgaatcccc atcaacagaa ccaaagttac
     4321 catgaccatc tactaaaagt gctctagttg aaaaatcttg tgccattctt accatagcat
     4381 aataaacagc agtatctcca tgaggatggt acttacctaa aacgtcccca acaatacgag
     4441 ctgacttcct gtatggttta tctggagtta aatttaactc actcattgaa tataatattc
     4501 ttctatgaac tggctttaaa ccatctctaa catcaggaag agcacgtcca gctataacac
     4561 tcattgaata atcaatatac gattttttca tttcttccgc tatttcaata gggagtattt
     4621 tgttattttc ttccatgtat actaatcccc tctctctata tatctaagtt tctaacaaat
     4681 cttgcatttt cttctataaa ttcttttctt ggagcaacct tatcacccat aagcattgaa
     4741 aatacttcat ctgcaattgc agcatcttct acagtaactt gtaataaagt tcttgtctca
     4801 ggattcatag ttgtttccca taattgctct gcattcatct ctccaagacc tttgtacctt
     4861 tgaagttcaa caccatttct tccaatttca tctagtaaga tatttaactc tttatctgag
     4921 taaacataat gttccttctt ttgctttgtc actttatata aaggtggctg tgctgcataa
     4981 acatacccct catctataag aggtctcata tatctaaaga agaatgttaa taataaagtt
     5041 cttatatggg ctccgtctac atcagcatcg gtcataatta taattttatg atacctagcc
     5101 ttatctatat caaaatcttc tccaatacca caaccataag ctgttatcat attttttatt
     5161 tcatctgaag atagtatcct atctagtctt gatttctcaa cattaagtat tttccctctt
     5221 aatggaagta tagcttgact atttctatct ctaccttgtt tagctgaacc tcccgctgaa
     5281 tccccttcga ctaagaatat ttcactttta gatggatctt tttctgcaca atctgcaagt
     5341 tttccaggta aagatgtact ttctaataca ctttttctcc ttgttaattc tcttgctttt
     5401 tttgcagctt ctctagctct ttgtgctctt aaagctttat caactatgat tcttgctgtt
     5461 gatgggtttt cttctaggaa agaccccagt tcatcaactg ttacactatc aactatacct
     5521 ctcataaagc tattacctag ctttgtttta gtttgacctt caaattgagg ttctggtaat
     5581 ttaactgata ctactgctgt aagaccttct cttatgtctt caccaagtag gttaacatca
     5641 ttttccttta aaaatttatt tctcttagca taatcattta cagttttagt aagagctgct
     5701 ttaaatccac ttaaatgaga accaccttca tgtgtattta tattgtttgc aaaagaatat
     5761 atgttctctg tataaccatc tgtatattgc atagctagtt caacaaaaca atcatcaacc
     5821 tttttatcta tataaactat gtcatcatgt ataccagttc tagatttatt taaatactta
     5881 atatattcta ccaaaccacc tgtatagtga aattcttttc ttttttcttg tccttctctt
     5941 ttatcttcaa atactatttt tattccttta ttcaagaaag aaagttctct aagcctatac
     6001 tccaatgttt catatttaaa ctctatttca tcaaatattg tctcatctgg cataaattga
     6061 atcattgttc ctttatcttg agattctcca actacttcaa gcttagatgt tggcaatcct
     6121 ttctcatatg tctgcttata tatctttccg tttaaataaa cctctgctac catccatttt
     6181 gacaatgcat taactacaga aacaccaact ccatgaagac ctccagatac tttatatcct
     6241 ccgcctccaa actttcctcc tgcatgaaga ttagttaata ctgtttctaa agttgattta
     6301 ccagttttag gatgtatttc aacaggtata cctcttccgt tatcttttac taaaacactg
     6361 ccatcttcat taatagatac atatatgtct gaacaatatc cttgtaaagc ctcatctata
     6421 ctattatcta caacttcata aactaagtga tgtaaacctc tagggcttgt actaccaata
     6481 tacatacctg gtctttttct aacggcttct aatccctcta atacttgtat ctgacttgca
     6541 ccgtattctt gtttcattta atttcctcca ttctctatgc taataacatc gccattttca
     6601 atattaaata ttgtaacatt tttttcatca aatatttttt tatgtgaaac ttcagcactt
     6661 gttataaaca tttgaacatt gcttaaatta tttactaaaa gtttttgtct tgcttcatct
     6721 aactcactaa aaacatcatc taaaattaat actggatatt cttcaacttc attttttatt
     6781 aattctatct ctgataactt taatgatatt gaagcagttc tttgttgacc ttgtgaacca
     6841 aaaagtcttg catctaaatc atttatgaat atatttaagt catctttgtg tataccatat
     6901 ctggtagtct tagattctat atcattaggt ctattcgatg acaacttggc taaaaattta
     6961 ttatatactg tatctatagt atcttcatca gttatattta tttgattttt atatcgaatt
     7021 gataatcttt caactccatt agtcaaattc atatgcatat tttcagatat attagctatc
     7081 ttttttataa agtctctacg caaaatataa atataacttc catactttgc taacgtatca
     7141 tcatatacat ctaataaagc ttcatcaaca tgtatatttt taagcaccct actcctttga
     7201 gaaagtgttt tattataatt ggtaagatat ttataatatt taggtataat ctgactaatc
     7261 tctttgtcta taaatgccct tctttcttta ggaccctctt taaccaacct taaatcctca
     7321 ggagaaaata taacaacatt taagttgcca agtagttctt gtatcttttg taaaggaact
     7381 ttatttattc ttatcccttt tttatctttt ccaataatta gctctattag actatattta
     7441 ttatattttg aaaaacttcc tccaatatat aagttttcac tattaaatct aaccatttct
     7501 ttatctttgt tagtcctaaa tgattttcca aaagaaagca tataaataga ttctactata
     7561 tttgtctttc cttgaccatt tttaccaaca agtagattta ctttcccatt aaattccaga
     7621 tgcaactttt tataatttct aaaattaaca agttgcaaac ttttaagttt cacaaaaagt
     7681 tctccttttt ttacaatact tttattttag ttccttctat ttcaactata tctcctgatt
     7741 ttatcttttt tcctcttctt aattctattt catcatttac tgtcactaaa ccttcttgaa
     7801 ttaaaaattt tgcatgacct cctgttgatg caatctctgc taatttaaga aattgatcca
     7861 actttatata ttctgattct atagttattt cagtcataat agttcctcct tgtcactatt
     7921 ttaaacaaat tcctattgat acactaaaag tatattatac cataaatatg cttataaaag
     7981 gagttgtcta agattagaaa aattttctac aaaagacaac tccttcatgt ctgctatata
     8041 tttgatgata ttcttactgg aagcagtaag taaatataat taacatcatc tgttggttta
     8101 attatacaag gatttacatt tgtagtaaat tctataaata tttcctcatt atctatattt
     8161 ttcaaacctt cgataaaata tctagaattg aaagctatat ctaggtaatc tccatctaaa
     8221 tctatttcaa cttcttcata cacatttcct ttttctgtat tagaagtaat tgccattact
     8281 ttatctctta tagataactt tataagatta tttttttctg attgagacaa taaagacgct
     8341 ctctctatac tatttaaaag ttccttagta tttaatttaa ctctgctgtt atgttctctt
     8401 ggcaataatt ttttataatc aataaaatct ccttcaagca atcttgtaat aattttcgta
     8461 tcattaatta taaaaatagc atttttttca ttaaatccaa ctttaacatt atcctctcca
     8521 gaaagcaaac tatttacatc tataagtgtc tttccaggaa ttatgacttt tatattttca
     8581 gttaaactat caaccgaaca actttttaca gctaatctat atccatcaat tgctactaaa
     8641 tttaagtttc tatctacaat ttctaaaagt tctcccatta aaacaggttt tgtttggtcc
     8701 tgtgagatag caaatacagt ttgtttaatc atatttttta ggatttcttg aggtatgcta
     8761 tataaatctt cttcatttaa ttctggtaat ttaggaaatt cttttgctgc ataaccttta
     8821 attttaaatc ttgaatttac acaatttatg taaatattat tttctgaatc agtttctatc
     8881 tcaacaaagg aatctggtaa ttttctaatt atatctccaa atagccttgc atctacaaca
     8941 acatcacctt tttctatgat ttctgcttgt acataagttt ctattcctat ttcagcatca
     9001 tatccagtaa gttttaattg accttcttct gtagatataa gtataccttt taataattct
     9061 attgttgttt ttccattaat agctttttga gcaattccaa ttctatttgc taggattttt
     9121 tgattacaaa ttattttcaa tgtggataac ctcctctggc tttttatata taaaaataag
     9181 ttaaaatcat agtagtaata gtagtaggtg ttgttgataa tgttaataag tatgtataac
     9241 attataaata caaagatatt aacatgtgta taagatgtta atatctttgt tattttagac
     9301 ttgataattt gttaataatt tttgttcaaa aaaagtatta acaggttatt caagagatat
     9361 aaacatacaa ttgtgtataa attatccctt caaatctgat ataattttgt ctattttagt
     9421 cttaatttct tcactttctt ctatatcctt agatactttg tcatgagcat gaattactgt
     9481 tgtatggtct ctacccccaa actcttcacc tattttagga agggataaat cagtaagctc
     9541 gcgcgttaaa tacatagcta tctgtcttgg ataagctatt gaacgtgttc tttttttaga
     9601 attaaaatct tccatcttta aattgaatac agaagatact ttttctttta ttctgagaac
     9661 atttatttct tcatttttag aagttgttat aatatctttt aaagcttctg tagcaagatc
     9721 aaaagatatt accctattgc taagagaaga ataagcaacc actctagtta atgcaccttc
     9781 tagttccctt atgtttgact taatattttt tgctatatat gacataacct catttggcac
     9841 atctatacgt tctagttgag ccttttttct aagtatagca attcttgtct caaagtcagg
     9901 tgcttgaata tcagttataa gtcccatttc aaatctcgat cttagcctat cttctagtgt
     9961 aggtatgtct tttggcggtc tatcactaga tataattatt tgtttgtttg cttcatgaag
    10021 agtattgaat gtatggaaaa actcttcttg agttctttct tttcctgcta taaattgaat
    10081 atcgtcgatt agtaatacat ctacgtttct atatttattc ctaaattctt cgtttttatc
    10141 gtcttttata gagtttataa gttcattagt aaatttttct gaagaaacgt aaactacttt
    10201 tgagtctttt ttttgactaa caatatgatg acctatagca tgcattaggt gagtcttacc
    10261 taatccaact cctccataca aaaaaagtgg gttataagct ttagctggag attctgcaac
    10321 tgcaacgcat gcagcatgtg caaatctatt actattacca ataacgaatg tatcaaatgt
    10381 gtattttgga tttagattgg ggtaaagaac tctataattt aaatctgtgc tatcagaatt
    10441 taaatcagca acctcttttt cgctcaatac aaatttaata ttatattttt ttaatgacag
    10501 ttgactaata gcatcttcaa ttaaatgtaa atatctattc tctaagatat ctttattaaa
    10561 atcactagga gccagtaaga ttaaatcatt aagatgtatt tttaatggta cgatgttttt
    10621 aaaaaaggta ttaaaactta ctgaagttaa gtcacctttt attaattgta gggttttgtc
    10681 ccataaagaa actatatcca taatataaac ctccgaacag aataattaat attttccaca
    10741 aagttattaa cagaaaagca aaaatatata taaattttac agaataacta aatttatgga
    10801 taatggtgtt gataaattaa taatagtata aattattaaa aaaatatgaa aagatataaa
    10861 caatatgtag ataaagttat aaataacata aacatacaat aaatggtgat tataactaga
    10921 gtattgtcag taatcttaaa agttatacac aaaagtatcc acaagctgtg aataatttct
    10981 aataagttga taacatttat gcacattatc aacaagtata tttataatat caaaatatat
    11041 gtcaaacttc aatgatttgt aactaattta tccacaatat taactaattg ttgataaaaa
    11101 tataaacatg ttaaaattac tttatttgtg tatataattg aaaaaaataa tttagaataa
    11161 aattaaatag atttttacta gtattttcaa taaaaaaaat acataatata tatatggtag
    11221 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
    11281 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
    11341 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
    11401 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
    11461 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggcc gctgcaaagg
    11521 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
    11581 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
    11641 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
    11701 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
    11761 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
    11821 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
    11881 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
    11941 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaagt atttatcgaa
    12001 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
    12061 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaga aatatggatt
    12121 tgtaaaaggt atgtatttaa cgataagaag aattttaaaa tgtcatcctt ttcatccagg
    12181 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
    12241 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
    12301 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
    12361 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
    12421 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
    12481 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
    12541 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
    12601 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
    12661 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca ccaaaagatc
    12721 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
    12781 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
    12841 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
    12901 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
    12961 agtaaagcta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
    13021 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
    13081 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
    13141 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
    13201 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
    13261 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
    13321 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
    13381 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
    13441 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
    13501 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
    13561 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aatctatatg
    13621 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
    13681 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aaaagctttg
    13741 aaggttgcag aagaaatctt taaatctatg tctggaaaga gtattgaaga atacaataag
    13801 agaacattaa tatatggaaa tatagtggat aatgaaaata taattgatga agttttattg
    13861 gcatatatga aaggaccaaa ttcctataca ggagaagatg ttatagaaat taattgccat
    13921 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
    13981 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
    14041 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
    14101 aatcaattag aaggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
    14161 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
    14221 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag acattaaaaa attatatgat
    14281 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
    14341 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagttact
    14401 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
    14461 ttaaaaattg ttgacacagc aggtataaga gatactgatg atattgttga aaaaataggt
    14521 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
    14581 tctagaaagt tgtcagaaga agatatagag atattagaaa aacttaagga taaacaaaca
    14641 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
    14701 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
    14761 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
    14821 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
    14881 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
    14941 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
    15001 atattccata atttctgtat tggtaagtaa tgtacaaaag taaacttgtt tttattatag
    15061 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
    15121 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
    15181 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
    15241 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
    15301 tcaattagtt aaagaaatag atgccttagg tggtcagatg ggattaaata tagacaagac
    15361 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
    15421 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgaaccaaa
    15481 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
    15541 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
    15601 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
    15661 ggggtatgca aaatatctta cagatagctt agttgagctt ggccttagaa tgagaagatt
    15721 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
    15781 acaagagggt gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
    15841 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
    15901 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
    15961 ttctatagaa gataaagtag taagatttag tgataaaaca tcacatcaat tatttataga
    16021 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
    16081 tgaagttcaa ttagatatgt acaaaactat aaagggactt gaaaattgta agataatgag
    16141 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
    16201 aataaagggt gttgagaact tatttagtgc agggcaattt aatggaactt ctggatacga
    16261 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
    16321 agagccattt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
    16381 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
    16441 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
    16501 taaaaaagat agatatgaaa ggttcctaaa taaaaaggct gctgtagaga aagaatttga
    16561 aagactaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
    16621 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
    16681 ttacgaattg cttgaagaat tagggaaagg cgctggagaa gatgtttcaa gagaagttaa
    16741 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
    16801 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
    16861 agaaggtctt agattagagg ctagacagaa actagatgat ataaaaccta tttcaatagg
    16921 gcaagcatct cgtatatctg gtgtttctcc agctgatata tcagttttac ttatatacct
    16981 agaacaaatc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
    17041 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
    17101 gagagatatt agttgactgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
    17161 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaagaatgga tatataaaaa
    17221 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
    17281 tatgtttaga tagcttagaa cttactttgt tagattcgtt aaataaaaga ataaactttt
    17341 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
    17401 aagattttgg taaagatgaa gactataggg aaaaatatga tgttgctacg gcaagagctg
    17461 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
    17521 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
    17581 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
    17641 atcataatat attagttttt aaaaaagtga tggaaacacc agttaagtac cctagaaaag
    17701 ctggtaaacc agccaaaaat cctattaagt aatgaaaaat ttattattgg taagcttaat
    17761 tatttctcag gaaataatta agcttactgt aaaaaaacaa aaataaagga attattaaag
    17821 tagatgcaga aataaatttg tatgtacata tagagaacaa aaataattta tatctaaaat
    17881 aagagaggta tgttatggaa gataaaagag ttatggagat acctatagaa gatgtagttc
    17941 caaatccata tcaaccaaga aaaatatttt cacaagtttc attggaggaa ttgagcaatt
    18001 ctataaaggt atatggtata attcaaccta taacagttag agcaaaagat ggcaaatatg
    18061 aactaattgc aggagaaaga agattaagag cagctaaatt agcagagctt aaaaccatac
    18121 cagcaattat aaataatatg aacgatgaat cttctgctgt tctagcgtta ttagaaaatt
    18181 tgcaacgaga agacttaaat ttcattgaag aagcaattgg ttatgaaaac ttaataaaag
    18241 aacatgcttt tactcaacag caattagcag aaaaattggg caaaaatcaa tctactgtag
    18301 caaataagct tagaatatta aagcttccaa acgatataaa aatgaagcta atagagaata
    18361 atttaacaga aagacatgct agagcatttc ttaaactacc aagtgaagat ttaatgcaaa
    18421 gtgtgttaga caaagtcata aaaaatgaat tgactgttaa aaaaacagaa aaattgatac
    18481 aagatgtatt agaagaaact aaagttcaag aagagcctga taaaaaacag aatataaagg
    18541 gtgctatgag tataagaatt tacataaata ctataaagca ggcctttgat gctatatcaa
    18601 atacaggaat tgatgctaag tataatgaaa tagataaggg agattatatg gaagttgttg
    18661 taaaaatccc taaaaaatag agattactgc ttaccagtaa tctttttttg taatataagc
    18721 cttgtaatat aaattgattt atagacatta tttatactta aaaaacacct attcaacatt
    18781 taaaattaat ttaattctta aaggggggaa catcatgggc aaagttatag ctgtattcaa
    18841 tcaaaagggt ggagtcggga aaactacaac aaatgtaaat ttaagtgcta gcttaggaac
    18901 tttgggaaag aaaatattag tattagattt agacccacaa ggaaatacaa ctagtggata
    18961 tggtattaat aaaaacgaag tagaaaatac aatctatgaa attatgttag atgggttaca
    19021 tattaaagaa gcaataataa gtactgaatt tgagaatgta gatgtagttc catcagctac
    19081 tgaactatca ggtgcagaga tagagcttac atctaaaact aatagagagt atattttaaa
    19141 aaattccata aaagcggtaa tagatgaata tgattatata tttttagatt gtccaccctc
    19201 actggggatg cttacaataa attgtttaac tgctgtagat agtgttttaa ttccaataca
    19261 atgtgaatat tatgctttag aaggtgttag tcaattaatg gagactataa aattagtaaa
    19321 atcaagatta aatgctgata tagaaattca aggtgttgtt ttaagtatgt ttgatggaag
    19381 agctaattta tcaatacaag tagttgaaga agttaagaaa tactttaaag gtagtgtata
    19441 tacaacttta attccaagaa atgtaagact tgcagaagct ccaagtcatg gaaaacctgt
    19501 aatttattat gataaaagat gtagagggtc tgtagcttat ttggagctag cagaagaatt
    19561 tatagattta gaagaggagg aatggtagta tggagaataa gtctaaaaga agtaatagat
    19621 taggtagagg tcttagtgca ttgataccag aaattaaagg agaaacttca gaaaaagaaa
    19681 tagtcaacat agatatagac aaaatatatc caaacgaagt tcaacctaga aagcaatttg
    19741 atgaagaaaa gataaaagta ttgtcggatt ctataaaaaa ttatggtgtt ttacagccaa
    19801 tagtggttaa aatggatgaa aataataaat atatgataat agctggagaa agaagattta
    19861 gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat aattaaagat atagatatga
    19921 aagatataat ggagatagcc ttaattgaaa acttacagag ggaagattta aattcaatag
    19981 aagaggctct agcatataag agtttaatag agcattacaa tgtaactcag gaagaaatat
    20041 cagaagctgt tgggaagagt agacctcata taactaatac actaagactt ttaaatcttg
    20101 gacaagatgt tatagaaatg atagacagtg gtagaatcac agcaggccat ggtaaggctt
    20161 tattgagaat tgcagataaa gatttgcaac ttcaaatagc gaaaaaaata gaagaagaag
    20221 aactttctgt aagagaagtc gaaaatatag ctaaaaaaat atctgaaaat aaacaagaag
    20281 aacctaaaaa atctaaacct aaggatgtat ttattttaga tgttgaagat aaacttagaa
    20341 atatatttgg aacaaaggtg aatatttcta aaggaaagaa aaaaggtaaa atagagatag
    20401 aatactataa tgatgatgat ttaaatagta ttgtatcaat gcttcttgaa tagaacaaaa
    20461 atataaaaca gagaggaaga ttgttatgat ttatttagat aatgctgcaa ctacatatcc
    20521 aaaaccagaa agagtgtata atgctgtttt agattgtatg aaaaattatt gtgcaaatcc
    20581 aggtagagca ggccataaac ttgctatgag agctgctagg gagatatatg atacaagaga
    20641 gaatatagct aagttgttca atgtaagtaa tcctatgaat atagtattta catctaatgc
    20701 aacagattct ttaaatttag cgataaaagg tgtattacaa gaaggagacc atgtaataac
    20761 aacgtctatg gagcataatt cagtaataag acctataaaa gcattggaaa aaaggggtat
    20821 tgaaaatact gtagtaaaat gcgattatga gggattttta gattatgaag atttagaaaa
    20881 atcaataaag agtaatacta aactgattgt aacaacacat gcgtctaatg tatgtggaac
    20941 tttaatagat ataaaaaaag ttggagaaat agctaaaaaa cataatatac tatttttagt
    21001 agatgcatca caaacagcag gtgtttatga tatagacgta aatgaatgta atatagatat
    21061 gttggcaatg ccaggtcata aatgcttatt tggacctcaa ggaacaggta tattatatgt
    21121 aagagaaggg ttgaacttaa atatattaaa agaaggtgga acaggtagta aatcagaaga
    21181 aatagttcaa ccagagttat tcccagataa atatgaatca ggaactcata atactccagg
    21241 tatagcaggt cttaaccaag gtatactatt tatatttgaa agaggaatca acaatataag
    21301 acaacatgaa gaagaattat gtcaatatat gatagacaaa ttagaggaag taccagatat
    21361 aaagatatat ggaccaaagg acagtaaaaa aagagcatca gtaatagctc taaatatagg
    21421 agatatggat tctggagagg ttactttctt attagatagt gattataata tagcgactag
    21481 atcaggaata cattgttcac cactagcaca taccacttta ggtactttaa aacaaggtgc
    21541 agtaaggttt agtataggat atttcaacac aaaagatgag atagataaag cagtggaagc
    21601 tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa taatatagaa ataaaaaaag
    21661 gggatatttc aaaataaaaa tttgaatcat ctcctttttt gtacaaaaaa gaaaaggggt
    21721 tcctaagaac ctctaatcta tttgataata gtttcaagaa attactttaa gtaatctcct
    21781 tcttcaagaa ctatatgacc actttcgtaa ttagaaccat caacagtaat ttttatttgt
    21841 tcaacattca tattttttat gtaagtacga gcaactgaat caagcattaa agtttctgca
    21901 tcactaccta agtttgactt tgtaaagtca gagctaacat ctaatatacc agtttttaca
    21961 ccatcaatgt cttttgtagt aaatgaattt aattttgcag attctggaac aactttaagt
    22021 ttttgaagtt cttcaaataa agtgttctca tcaattttat ttaaatcaac tttattttca
    22081 attaaatctg tcttatctac atcaaaagag taaatagtag ctgattgagt tttagtttct
    22141 tcttttggag taggtttagc tttgttatta ggaacctctt tttcagtatt gttttcttct
    22201 tttggttgct caacatttgt attgctctta gtttcttttt tagatgaatc acttccattt
    22261 tgagcattag tacaaccaac tgcgaatata gatatagata atatacttaa cactaacatt
    22321 attttcttat ttttcataaa aatttctcct ctcttaaaaa ctattcttat atataattat
    22381 agtcgttatg agcaaatata tgaatacaaa atagtaacaa atttaaaaaa tgatttattt
    22441 aaattaataa aactcaaaat aaactaaatt ttagtcattt ttatgggaaa tcatttaatg
    22501 tacagtgaaa atattgtaaa ataaaagtag tgtttttaaa ataaatataa gttataatta
    22561 tattataaaa aaatgtcttc aaaaggtggg aatagtatgg aaattttgag cttaggagaa
    22621 aagattaaaa aattaagaaa agaaaaaaac atgacattaa aagaattagc tggagatagg
    22681 ataacagcag cacaaataag ccatatagag agggataaat ctcatactag ttatgaactg
    22741 ttggaatatc taggcgataa gctagatgta agtgtagaat acttattaga gaccaaggaa
    22801 atgcaatcaa aaaagataac tgataattta atactacaga gcgaaattta tataaaagaa
    22861 aatgaattaa acaaggctga ggaccaaata aaagaagttt tagatatatg taagagatac
    22921 gatttaatgg agaactatgg cagatgtaat tttttaatgg ctaatattaa tttgaaaaaa
    22981 tctaattata atgatgctat agaaaacttt gaaaaggcat tgtacttctt tataaaaaat
    23041 agtgatagtg aaaatatact taagtgttat ttaaacatag ggaaaatata tatgcaggaa
    23101 gacttttata aaggtgctat aagccacttt aattttgcag aggaaatttt gtcagagaat
    23161 aaatttgagg atgttaacat acacaaggaa ctatatagta agatggcata ttgttacata
    23221 aaactagata atccaaatat gtcattgaag tacatagaaa agataaatga aatagataat
    23281 agaaatgata gaaaagaaga tgtcgatatt ttggtgttaa aagcaaataa tatgcttgag
    23341 attggaaaat atgaagaatc aagagattac tttaaaaaag cactaaaaat acttgagagt
    23401 gaggataata aaactggatt agcaaatgtg tatttaacaa tttgtgatgt ttacagaaaa
    23461 ataggtgaaa cagatagagt acttgaatat tcacaaaagg tatatgacat aaagaaaaat
    23521 gatgaagatg aatacatgat gtcaggatta tttaagataa tagaagcata tatagataaa
    23581 gaagattatg atttagctag aaaatattgt aagatagcct tagcttcatc aataaaaaat
    23641 aaaaataaat tcaatgaata taaagcattg agattctatg ctaatatgta taaaaatcag
    23701 aatgaagtaa ctcttgcaat tgaatatctg attaaatgca taaaaatagt atcagattta
    23761 ggcaacaata gaatattggc caatttatat atagacttag gtcaattata ttcaaatata
    23821 tcaaaagaaa aagagttaga atattatcaa aaaggtgtat ttatgtacaa aaatttagaa
    23881 ataatgtagg gtaaataaac ctataagggt atagaaacaa tctataacaa gcaagataat
    23941 atctatgata aaatataaat tgtatgaata tttttaagga gatgaatcag acaatgagta
    24001 taaaaataga tgcgagaggg cttgcttgtc ctaaaccagt tataaataca aaaagagaat
    24061 tggataacct agaagaaggt gttgtagtta caatagtcga taatgaaaca gctaaggaga
    24121 atatattaaa acttgctaaa tctttaagtt gtgaagcaaa tattgttgac gaaaaagaga
    24181 attttatatc tatagaaatt agaaaaggta aaaatatact agataaacaa aatgttgtag
    24241 ataataaaga aagtgaatta gacaatacat gtatatttat atcttcagat aagatgggac
    24301 ttggaaatga cgaattagga aaagtgttaa taaaaggatt tatatataca ttaacagaat
    24361 ctaaacctta tcctaagtat gtacttttag ttaatggagg agtaaaatta agtgctgaaa
    24421 atgaagaaac catagaaaat ctaaaaatac ttgagaatat gggtgtagaa gttttatctt
    24481 gtggtacttg tttagattat tataatttaa aagacaaatt acaagtaggt acagttacaa
    24541 atatgtatac tatagttgag acattaaaga atgcatcaaa taccatttct atataatgta
    24601 tgtttgttta ataatataaa attaataata aagattaact caaatagata gtcagaaagg
    24661 attaagtaat gaatcaaatg tatatagtgt catttaattc gacacatcat gcgataagat
    24721 cagaaaagtt atttggtgaa aatagtctaa aagtaatggc tcttccaaca ccaagagaaa
    24781 taacagcaag ttgtggaata tcaattaaat tttcttttga agatatggaa aagataaaaa
    24841 ctattttagt tgaaaataat gtagatataa aaggaattta ttgtataagt aaattagaaa
    24901 atggctcaaa agaagtagag aagttagatt aggaggctaa ttatttatgc caatggattt
    24961 aaaaataggt gatattgttg aattaaaaaa gcaacatgca tgtgggtgta aagagtttga
    25021 aatcataaga acgggaatgg atataaagat aaaatgtact aaatgttcaa ggctaataat
    25081 gcttgataga gagaccttag aaaagagagt aaaaaagcta ataaaaaagt gataaaaatt
    25141 taacacttaa aaaatgtcaa ataccgtata ttaatatcca aaattttggt ataataagaa
    25201 ttgaagaaaa cattttcatg ggggtgcttt aattatggca gttaaatatg caaaaagaat
    25261 gcaaggatta caaggatcag aaatacgtga gcttttaaaa cttactcaac aaccacaaat
    25321 aatatctttt gctggtggaa tgccggctcc agagttattt ccagttgagg aaatgaaaaa
    25381 agtatcagtt gcagtgcttg aagaaaatgg aagatcagct atgcaatata ctacaacaga
    25441 aggatatgaa ccattaagag aaaaaatagc agctagaatg aatgataaaa acaaaactaa
    25501 tgtaaacaaa gatgatatat tagttacaag tggttctcaa caaggtctag attttgcagg
    25561 aaaagtattt attgatgagg gtgatgtaat tttatgtgaa agtccatctt acataggagc
    25621 tataaacgca tttaaatctt atcaacctaa attcatagat gttccaacag attctgatgg
    25681 aatgataatg gaagaattag aaaagatact agaaacaact gatagaataa aaatgattta
    25741 tgtaatacct gacttccaaa atccaacagg aagaacttgg ccacttgaaa gacgtaaaaa
    25801 attcatggaa atagtaaata aatttgaaat accagtaata gaagataatc catatggaga
    25861 tttaagattt gaaggagaga ctttaccatc tttaaaatca atggatacaa aaggattagt
    25921 aatattctta ggaactttct ctaaaatatt ctgcccagga tacagattag gatggacttg
    25981 tgcttctcca gaaattttat ctaagtttaa ctttgcaaaa caaggtgcag acttacaagc
    26041 atcaactata tctcaaatgg aagtaagcaa atttatggat atgtatgacc tagatgcaca
    26101 tgttgacaaa ataaaagcag tttatgttaa acgtagagat gtaatgctta agactatgga
    26161 agaagagttc ccagaaggat tagtatttac tcatccagaa ggtggattat ttacatgggt
    26221 agagcttcca agtaatttaa atgctaaaga attaatgcca aaatgtttag ataagaatgt
    26281 tgcttatgtt gctggaggag gattcttccc taatggtggt agagaaaata ctttcagact
    26341 taactattca aatatgccag aagaaaaaat aatagaaggt ataaaaaata tagcagctgt
    26401 attaaaagaa gctatgggtg tagaagctta attaaaaata ataacttata tataatacaa
    26461 gggatggttg ttgccatccc ttgtttgttt gaaaagtata taattcacct tatactaaac
    26521 tttgtccaaa atttaaattg acagaaataa gaataaatga taaaatgtgt aggtatgaga
    26581 ttttagtctc atttctctgc tctaagagtg tagagccgtt aagtccaaag ggaggtgaat
    26641 tatagtgaga aattatgaat tagtttatgt ggtaaagcca aactctgatg aagaagtaag
    26701 agaggctata ctaaacaaag ttaaggaagt tgttgcaact gacggagaaa tagttaaagt
    26761 tgatacttgg ggaactaaaa aattagctta tccaatagct aagtttacag aaggtttcta
    26821 tgtattagtt aacttcaaat cagcagtaga cgttcctaaa gagatagaca gaaacttaaa
    26881 gataaatgaa aacgtaataa gacacatgat agttgttgct taataactgg ttttattata
    26941 aaaaataggt ggtgttttta tgaatcaagt tgtattagtg ggaaggttga ctagagaccc
    27001 tgaattaaga tacatcccag gtacaggaac accagtggca tcctttacaa tagccattga
    27061 tagagactat gtaaaaaaag atggatctaa agaaactgat tttatacctg ttgaagtaat
    27121 gggtaaatct gctgaatttt gtgctaacta cataactaaa ggtagattag tagcacttca
    27181 aggatctata agagtagaca attatcagac acaatcaggt gaaaagagaa cttttacaaa
    27241 agtaagtact agatcagtac aagctttgga tagtaagaat aaatcggaaa attcatatag
    27301 agagagtgct ccagcttttg agccaagctt tgaacctcaa ggtttagacc cacaaggttt
    27361 ccaagctata gatgatgacg atataccatt ttaaaaagga gggaaaaaag tcatgatgaa
    27421 taaaaaaaga cgtaagaaaa aaagagtttg tcaattttgt gctgacaaaa acgctaaaat
    27481 agattacaaa agtactcaaa gattacaaaa gtatataact gaaagaggaa agatattacc
    27541 aagaagaata tctggaactt gtgctaagca tcaaagagaa ttaacagttg ctataaaaag
    27601 agcgagaaat atagcacttt taccatatac attagactaa aaaaatgagc cttgtgctca
    27661 ttttttatta tatgtattta tatatctaaa tgaggcgagc tataaagctc gtttttttgt
    27721 ttatatactt aaatatatta ttatttatga tataatatta tatattaagg agggttgtaa
    27781 acgtgaaaat agataaaggt tctgagataa cgagaaacat aaagataata gaatggatga
    27841 aaacagaaat attaatgagt gtaagtgatt tgtttaattt attgtttaaa ggggtaaaac
    27901 cattagatga agcaattcaa gatactttag caaatataat aatgataaca tatctcttag
    27961 caaagagatt aggaattagt tttagagatg tggattataa agtcaaggaa aaaataaaaa
    28021 ttggtataga tgaggaccat agcgttgaaa gatggtatgg agatttatct aatttaaaaa
    28081 aacatatgga taatagggag tgatattaaa ttgaataata aaattagact atctaaggca
    28141 atgtctataa tagttttagc tataataatc gctttagtaa tagcctatat tccaatgcta
    28201 ggaatattta gtatgttagc agctgttcca tatgtaatta taggtgctat tactgataga
    28261 aagtattctc ttatatctat tttggttaca ttttgtgtac tgattctatt tgcagacatt
    28321 tcttatgcat taaatatatg tataatgtat tctttaccag gtatagtaat aggaaagatg
    28381 ctaaagagaa gctttgaaca agaggacagt aataaattcg aaccgatata tggtggaacc
    28441 ataatatttg tactttcaat gcttgtatat ttttttgtat taaagacatt tttaaatgtg
    28501 aatctattag atgaagtttc taagatgata tcagaaattg taaatattca aaaaaatagt
    28561 ttttctgcaa ctgaacttaa agtgtttgat aaaatgaagc cagatgagat tgtaagttat
    28621 tttacaaata tgctaccaat gatgttattt ttacaagggt tgttatcagc gtttgtcaca
    28681 tattatttga gtgtattttt tataaaaagg attacaaaga tgaatataag atttcctaag
    28741 tttgctgatt tttatttacc gggaaatgct atactcacaa ctttcttact ttatttatta
    28801 gtcttattta ttcaaattat tggttcaaaa ttatatacag aattaataat gactaattta
    28861 cagctagttt ttaatttaat gtttgtaatt caaggtatag cagtttgcat ttatttctta
    28921 aagaaatgga taagacaggg accaaataaa attatgtttt tttcagggat aattttatgt
    28981 ttatttggat ttatgggaat atcttttgtt ggtatggtag atagtataat tgactttaga
    29041 aaggtaagaa gctataaatc cacttaggag gatagaaatg agtaataaac aaacctttaa
    29101 attaaatatg ccagaaataa atttatatat aattgttatt ggtatttcta gtataatttt
    29161 gctttattac aacttgtatg tagggtgttt atttttctgt atttttgtat atatggtttt
    29221 tcacaattgg agaactacta atattagacg tcatgaatgg acagagtata ttcaaaattt
    29281 atctttagat atagatgaaa caactaaaaa agccataata aatcttccaa taccattatg
    29341 tattttagaa tttgatggaa atatatcctg gtataatgga aagttttatg atatgattgg
    29401 tcaaaaagat ttacttgata aaaatataga ggatatagtt aaaaatctta atttaagaaa
    29461 agtattaaat gaaaataaag agatgtacac agaaataaac tataaagaaa aagaatatac
    29521 aataatttat aatgttataa aaaatgatca agaaaaaaat cctaaatatt tgatgatatt
    29581 atactggata gacaagactg aatatttaaa agtaaaacaa aattatgatg atgaaaaaaa
    29641 tgcaatgatg ctaatacaag tagatggata tgatgaagta ttaaaaagtg cagcagaaga
    29701 taagagagct ttaattaatg ttgaagtaga gaaaatactt tctgctttag aattaaattc
    29761 aaatggtgca ttgagaagaa cttcaaaaga caaattcttc ttggtaatgc ataaaaaaga
    29821 gttaaaaaaa ttggaagctg agaagttttc tattttagat acaataagac atattgatta
    29881 tggaaataat cttcctgtta ctataagtat aggaattggt atagatggag atacattaaa
    29941 tgagaaccta aaacttgcta cgggtgctct cgatttagca cttggtagag gtggtgacca
    30001 ggctgttgta aaaacaaaag ataaatttgt gttttatgga ggaaaatcta aagctgtaga
    30061 aaagaaaacc aaagtaaaat ccagattaat aggtcatgct ttaagagagg ttatacaaca
    30121 aagtgaccaa gtttatataa tgggacataa atatcctgat atggatgcta tgggagcagc
    30181 agttggtgtt tatgatatat gtaaatcctg taacaaaact gcaaacatag ttttacaatc
    30241 tgtaaatgaa tctatagaaa tatttataaa taaaataaat gaaaataatt actataaaaa
    30301 gttgtttatt ggaaaagagg aagctattga caactgcact aaaaatacat tagttgtagt
    30361 agttgatact catagaccaa attatactga atgtgaagaa ctgttaaagc tatcagagaa
    30421 ggtagtagtt atagaccatc atagaagagg tgtagagttt ataaatgatg cagtgctttt
    30481 attccatgag atatatgtat cctcaacgtg tgaaatggtt acagagttag ttcagtatat
    30541 ggatgaagat gtaacaataa ataaacttac agctgaaggg cttttagcag gtataagtct
    30601 agatactaaa aactttgcat tcaaaacagg tgttagaact tttgaagctg cttcttacct
    30661 tagaaaagtt ggagcagata ctatagaggt aaagaaattc ttcaattctg atgtaaaaga
    30721 tttcataata aaagctgaaa taattcaaag tactaaaata attaataata gaatatgttt
    30781 agcatattca agtactgaga tagatagtat aaatgttata atagctcaaa ctgctgatga
    30841 gttattaaat atcaaagaag tagaagcttc ctttgtatta ggtgaaaaag atgatactat
    30901 atttataagt gcgaggtctt taggacaaat aaatgtacat gtacttatgg aaaaattagg
    30961 aggcggggga cacatagata tagctggagc acagttaaaa aatgtttctc taaaagaagc
    31021 ttataagatg gtaaataaaa taatagagga gtatttggag gaggaagaat agatgaaagt
    31081 tatattatta aaagacgtaa aaggaactgg taaaaaggga gaaatgaaag aagtaagtga
    31141 tggatatgca agaaatttct tatttcctaa aaagatggct gtgcaagcag atagtgtagc
    31201 aataaaagaa ttaaatgaaa agaataaatc taaagaaata aaagcacaaa aagagtatga
    31261 agaagcagtt ttattaggta agcaaatgga agaaattaat atagaaatat attcaaaagc
    31321 aggagaaggt ggaagattat ttggttcaat aacttctaaa gaaattgctg agcaattaaa
    31381 gaaacaaaaa gacatagatg tagataaaag aaaaatatta ttagatgaac caataagatc
    31441 tttaggttca actttcgtag aaataaaaat acatcaaaaa gtaactacta aaataagagt
    31501 ggatgtaaaa gaaaaacagt aataactaga ggtgattaag atggaagata tgacgagaat
    31561 tcctcctcat agtgtagaat cagaacaatc aatattgggt tctatacttc tagataaaga
    31621 tgctataata acggttacag aaactataaa gcctgatgat ttctataaag aggctcacaa
    31681 aataatatat gaatgtatga taactttaag taataaaggt gagcctatag acttgataac
    31741 attaacagaa gaactaagaa aacaagggca tcttaatgat attggaggaa tcagttatat
    31801 tacaagtctt tctactatag taccgacgac ttcaaatgta aaatactatg ctgatatagt
    31861 aaaagaaaag tctgtactta gaaagcttat aaaagcttct aatgaaataa taaatctagg
    31921 atatagtgga gcaactaaga ttgaagatgt tttagaacaa gctgaaaaga gtatatttga
    31981 tatatcacag gagaaaacta gtgatgattt taaatctata aacttagttt tgatggatgc
    32041 ctatgatatg atagaaaaat tatacactaa caagagtgat gtcactggta taactactgg
    32101 ttttaaagat ttaaacaaaa aaataaatgg tcttcaaagg actgatttaa tattaattgc
    32161 agctagacca gcaatgggta aaactgcctt ttctttaaac ttggttcaaa atgcagcctt
    32221 aaaaggtgat gcctctgtag ctgtatttag tttagagatg tctaaggaac aattagtaca
    32281 acgtatgtta tcttcacaat ctagtgtaga attaaaaaaa ataaagactg gaacacttaa
    32341 tgataatgat tggcctagaa ttatagatgc tatggcagta ttatcagatg ccaaaataca
    32401 catagatgat actcctggta taaaaatatc agaactaagg tcaaagtgta gaaagttaaa
    32461 aatagaaaaa ggactagatt tagtgcttat tgattatctt caacttatgg aaggtgaagg
    32521 taataatgaa agcagacaac aagagatatc taagatatca aggtcactaa aaatattagc
    32581 aaaagaatta aactgtcctg tagtagcact atctcagtta tcacgtgcac cagaacaaag
    32641 agcagaccat agacctatgt tatctgattt aagagagtct ggagcaatag aacaggatgc
    32701 agatatagtt atgttcttat acagggacga atattatcat gctgattctg agagtaaaaa
    32761 tataggagaa gtaataatag caaaaaatag acacggtgaa actggttctg tggaacttgt
    32821 ttggcttggt gaagttcaaa gatttgggga taagttgaga gacctataat ctaacgaata
    32881 tccacagaac gtcctttctt tctacaggaa aaatgtggat acatgtcact aaatatgtcc
    32941 aaacattgaa atttagctaa aaaactatcc acactatcca cagatggcct gttgataagt
    33001 gtgaataagt agataaagca agcttttggc ttgctttatc tgtgtataaa tattaattat
    33061 taggaggaat aatatggaaa aaataagctt aaaatatata tatcccaata taattaaagt
    33121 acttgatgaa ataaacttat ttagagtaat tgacaataat ttaagagaat ctattgttgt
    33181 ttatgctaat aatgtggata atcagtatca tataaacatg actaatacaa actttggaaa
    33241 tataattaac atatgtaaat tagaaaaact tttagatgtg gataaattta tggaaaaagt
    33301 tataaaatat gaaaaagaaa taatagagaa agaagagttt tcaaaaatag aagaatatat
    33361 gttaaatatt ggagaatact aaaaaagttg aaaaaaatac gaatgtttga tagaataagt
    33421 aagtaaagtg ttcgagagta tataaaatat agaggtgaat tacatgaaaa cagttgcaat
    33481 tgttggttct caatggggag atgaaggtaa aggtaaagtt atagattatc ttgctactca
    33541 agcagatgta gtagtaagag ggcaaggtgg aaataatgca ggacatacat tagttgtaga
    33601 aggtaaaaaa tacgcattac atctaattcc atctggagtt ttaaatccaa atacagtgaa
    33661 tataatcgga aatggaatag tgtttgaccc taaaggattt ttagaagaat tggaaatgtt
    33721 taaaacggat aatataagta cagaaaatat aaaaataagt gatagagcac atgtcatatt
    33781 cccatatcat aaagaactag atgcattgtc agaagaagcc agaggcgatt taaagatagg
    33841 tacaacaaaa aaaggtattg gaccttgtta tatggataaa acagagagat ctggtattag
    33901 aatatgtgac ttaatggata aagataaatt tgctataaaa ttaaaagctc aaatagatgc
    33961 taaaaatgaa atagttaaaa atatatatgg aaaagaagaa ttatttgatt ttgaaactat
    34021 atataacgaa tatctaggat atgcagagca aataagaaaa tatgtggcag atacgtctgt
    34081 tattgtatat gatgcagtaa gagcaggtaa aaaagtattg tttgaaggag cacaagggac
    34141 tttattagat ttagatttag gaacttatcc atttgttaca tcttctcatc caacatctgg
    34201 tggatttgca attggtgcag gtatagggcc aaatatgata aaagacgttg ttggtatagt
    34261 aaaagcatat actacaagag ttggagaagg accgtttgtt acagagcaaa taaatgaaac
    34321 tggagataag ataagagaac aaggacatga atttggggta actactggta gaccaagaag
    34381 atgtgggtgg tttgatgcag ttatagttaa gtatgcagct agagttaatg gattgactag
    34441 tatttcattt atgttacttg atgtattaac tggatttgat aagataaaag tttgtacgtc
    34501 ttataaaatg ggcgataaga taataacgga tttcccagct tcattagatg atttagcaaa
    34561 atgtgaacct gtatatgaag aattagatgg atggaatgaa gatataactc aaatagataa
    34621 ctttgatgac cttccagaaa atgcaaagaa atatgtagca aaaatagaag aactagttgg
    34681 agtaagtgta gatatggttt ctgttggtcc aaatagagct caaactataa taagaagaaa
    34741 tatatttgct taatatttta tcaattaaat aatttaaaat agttgaagaa gagtatctca
    34801 agataatgtt tatagttatt agagatgctc ttttatattt gacttacatt gattttttat
    34861 taagttaaag ctacttatac atattctaac tgaaattaaa gttgaattta tgtagataca
    34921 tataggtttt ttaaaaagaa ttttgttgaa aataaatggc taaagatgat tttaattata
    34981 ttctaaaaaa agtaaaaaaa actattgact aaaaattata tagacgctat aatataactt
    35041 gtgagtgaaa gaaacgacga aaaaagtcga aaaaatgtgg tctattagct cagtcggtag
    35101 agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaatat
    35161 ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg cggtaacagg ggttcaattc
    35221 ccctacgagt caccagtatg ggactatagc tcagctggga gagcacctgc cttacaagca
    35281 gggggtcaca ggttcgagcc ctgttagtcc caccaaattt tgaattagag ataaatacta
    35341 gattatatgc tggtgtggct caacggtaga gcagctgact tgtaatcagc aggttgtagg
    35401 ttcgattcct atcaccagct ccacaaagta cataaattca aattaatata attttctatg
    35461 ttacaggata cttttaagta tcttttttta atttcatttt tattgtatta aaaatgaaat
    35521 taaacataca acaaaatttt gaattacttg caatgtgata gttttaaata taaaatttag
    35581 aatcattcta gcataatatt atgtaatata gcaaatttaa tttatagtat caataaatat
    35641 agactactaa gaatgtaaat gatttgttag attaaggtac attagatatg taaatagaaa
    35701 aataaaaagt atgttttaat ttatgtgata taagttttaa acagagaagc aagtttgctc
    35761 ttacctctct atttaaaaat aaaaaaatgt tttttaaggg ctattttatt taaatctttc
    35821 ccatctcaaa tcatctccag taaattctaa aatcctaaac ttatctaata ttctagagag
    35881 agttctttga gtgtaagtat ttcctatttg agatggtgtt aagttggttg aaataatgat
    35941 ttttttacca gcaacaagtc ttgtatttac aatattgaag atttctccac tagtaaaaga
    36001 attattaagt tcagttccaa ggtcatctat tattaataaa tcacaatcaa acaagttttt
    36061 ataattatct tcacttattt gattatttgt gtctcttcta aatttataat cttcaagaat
    36121 atctaaaatt ctaaaagaag tttgatagat tacaacattg cctttatcta acaattcctt
    36181 tgcaatacaa ttgcacatat aggtctttcc tagtccagta gaaccataaa acagtagatt
    36241 ttcactatta tccttcttaa aatcatgaac aaaattttcg cagatactta aattatttag
    36301 catattttct ctaggagata tttcaccatc agagcctttc ttaggagaaa atatattcaa
    36361 gttaaaattc gaaaaatttt cctgacttaa aatcctatct aaattagaca ttttataagc
    36421 ttcatttaca atttcttgtt tgaggcaaga acatttttct ccattaggta aaaatccttt
    36481 atctttacaa gaaatacatt gaaactttat ttccaagtaa tcaagaggta tgttactttc
    36541 agctaaaagt ctttctttct ttacttttaa agattctata ttctctttag tcttctttac
    36601 aatttcatct ttagacttag gatttaaaag tactatctta gctaaactga gacctatttt
    36661 agaaatttca tcatctatag attttatctc agggattcta ttatatacct cattttttct
    36721 atgttctaat aagagttcat tatcatctct tctcttagca tatttagcaa gtatttttct
    36781 tattttatcc tcattcattt aatcacctac ttaaactttt ctttttgact ttttttaata
    36841 atctcatcga gctcatcaga ggtatactga gtgaatgttt cgttgaaatt atgaaactta
    36901 gtttttttgt aagtattgtt gttttgagta gtattaatct gcttcttatt aatattctcc
    36961 ttaactatac gttcttcctc tttttgcttt aaatcattta aattttttat atttttttca
    37021 ttccaatttt taattatacc atttatataa gatatacttg ggtttgaaat attcttagac
    37081 ttagaacatg catttattat caaatctata tccatattaa atttatcaaa ccaggtgtcc
    37141 ataagttctt tttctgattt tgatggttgt cttgaaaatc ccaattcatt gaaaatcgtt
    37201 ttatataaaa tatatcgttc acttcttacc aaaaagcttt cttcaacatc ttttggcgta
    37261 tataaatttg aatcatacca atttctaata atgccttcaa tgtatttaac tggctttgat
    37321 gtaccagttt tatctttgac gtattcatat gcgcaaagta tcatatcggg gctcatattg
    37381 tatttattca ttatatccat aatactcatc ttttcacttg ggtcgagata tcgcccaact
    37441 attttattta tagaattaaa catttttcta atacttggat tttcagaagt agatacaact
    37501 ttatctgtat tagattttat agatgaatta ttgcttaata tattctcaat atagaaattt
    37561 tttaaatcta aaaattctat tgaataatta aaattatcat attcttcatt gtcatgtatt
    37621 tttataatct tcttttcttc ccagaatttc caagcagata acacatcaga caatggtata
    37681 tttaggtttt tggctattga attattgtcg aacttaggat tagaagtaat atcacatgct
    37741 tgtctatatc ctaaaagata aacttttacg tataacccgt ccgccattgg cataaatata
    37801 tctataaaga tattgggaat agtgatttca cctaaatcta tttcattgct ttctttaaaa
    37861 aacattttat cacctcagat taaattatat cattttttat attatatttg tctgtaaaca
    37921 aataaaaggt cacaagatta gcatgtaaat attttctaaa agtattcatt ttgaaaagga
    37981 ggagatattt tggttataaa attcaaccaa aaagtaagaa gaacattatc agtagcgatt
    38041 ctatttatgg tattaataat aggttgcttt atagaaaagg atgacagttt agaaacatac
    38101 aaagaaaaaa ctaattcaaa aataaatcag tataatatgg atgttatatt tgatgatgag
    38161 actaagaggt taatgtgcaa tcagaatgtt gactacataa ataatacaaa atcaaacata
    38221 gataaaatat atttccatat atatccgaat gctttttcca agaaagattt tgctcctttt
    38281 gaaaaaagtg aaatgacaag ggcttatcct aatggattta acgaaggata tatagatata
    38341 aagaatatat taaataataa tagtaaaatg gagtataaaa ttaaaggaga caaaaatgac
    38401 atattagaaa tagaattagg gaaagagtta aaaccaaatg aaagaatgtc attagatata
    38461 aaatataacg taaaaatacc aaattcaatt ggaagatttg gatatggaga aaatactata
    38521 aatgttacta attggtttcc tatagcttgt gtaaatgatg aaaggggatg gaatttaaag
    38581 agttatgaaa ctattggcga cccattttat agtgagacta gcgattttca tgtgaaacta
    38641 ttgattccaa ataaatataa aattgctcat acaggaaaac ttattgatga taaacatgat
    38701 aataagaaaa tgctatatga aatagaagct gaaaacgtta gagattttgc atttatacta
    38761 agtagtaaat ttgatgtaaa caaagtagat tcaaagggag tagctatcaa tacatataat
    38821 ttaaataaaa agctttctaa aaaagcaact gaagtggcaa aagattcaat aaatatattt
    38881 agtgaattat ttggaaagta tccttacaaa gaattttctg ttgtagcaag tgactttttt
    38941 atagggggga tggaataccc tatgcttgtt atgatagacc aaagtttata taatgaaaaa
    39001 aatgaatttt tactagaata tgttattgcc catgagacag cacatcaatg gtggtattct
    39061 gctgttggaa atgatgaaat aagtgaaccc tggttagatg aagcacttac tgaatattct
    39121 acaatagttt attttgaaca aaagtatggt aaagatatgg caaacaagct tataaaaact
    39181 atggagatac agacaaaaag ttatttgagt gaaaatatat ttaagccagc aaatgagtat
    39241 aaaaattcta ctgaatacag cttgaatgtt tatacaaaag gcgcaatagc atttaatgaa
    39301 ataagaaaag aagttggaga taaagtattc tttgagactt taaatgagta ctataataaa
    39361 tataagcata aaaatgctaa tggaagagct tttgtggaac tatggaatag taaaggtgta
    39421 gatataaata agattataag tgaatgtaag taaatagtga atgtaagtaa attttgtgca
    39481 gagtattaaa ttttatttaa tactctgttt ttttggtata atataattta taaggcagaa
    39541 aaagaggtga aggaatgcta aagtattgtt cagtagggag tggtagtagt ggtaattgcc
    39601 actatatagg gtataaaaat actaatatac tagtagatgc aggattaagt ggcaagagaa
    39661 taactacagg tcttaaagac attggtgtag atgcagataa attaaaagga atatttataa
    39721 cacatgagca tgttgaccat ataaaaggag caggtatatt atcaagaaag tttgatatac
    39781 cagtatttgc taatataaaa acatggtgtt ctatgaagga caaattaggt gacatcaaag
    39841 ataaaaatat gaaagtattt gaaaatgata aaacctactc attaggtgat atcatagtaa
    39901 gacctttttc aatagaacat gatgcgtctg acccagttgg atataatttt tatacagaga
    39961 gtgatgaaaa aatgtctata gctactgata taggatgtat aactcaaaat ataaaaaaac
    40021 atctatataa atctaaatta gtagtattgg aatcaaatta tgaccctaat atgcttatga
    40081 tgggttcata tacatatgct ttaaagaaaa gagtaatgtc aagtactggt catttatcca
    40141 atgaagatgc agctaatttc tgcgttgaac ttattaatga aggtacagaa tctatattat
    40201 tagcacattt aagcaaagaa aataattttc ctgagttagc gtatgagact tcaaaaggag
    40261 tattagcatc aaatgatata gtagttggac aagatgtaaa actagatgta ctatcacgaa
    40321 atgatgtttc taatgtttat gagatgaagt agaggtgaat aactttgcta aagagaaatt
    40381 ttgaaaatgt tgaggataat aaaattttat actctataaa gcagggtatg attttagcga
    40441 tacctgctat tatgactggt tcaactgctc ttgtaatttt gaatcttcca ataaaggctt
    40501 atcaagaata tttatcaggc ttgtttaatg gagaggttac aaatattctt aactttataa
    40561 atgattctac tttaggaatt atttcattaa taatattatt aacaataagc tatagttatg
    40621 gaaaaatata tgggagcaag tatacagtac ttgttcctat agttgcaatg tgctcttttt
    40681 tagtattttc acatggtaat gagcttaatt cttatataga aatatttaaa acaaagtggt
    40741 tatttacatc tataattgtt tctatgacat cttctgtatt gtttgtaaaa cttacagaat
    40801 cttttgttac aagtgtaaag tttcatacag aaggggctga tgcagacttt aatatggtag
    40861 tttcggctat agttcctttc ataatagtag tatttttgtt ttctattttt agagtggtaa
    40921 tgataagtct aattggcagt tcaaactttc aagatatatt ttataattta ttttcaaatg
    40981 tttttaataa aatgggaaca aaccttatga gtgctctgtt gtttattttt ttaatgcact
    41041 ttatgtggtt ttttgggatt catggtagta atgtattaga taccgtcgca aaaaatttat
    41101 ttgaaaatag tatggcaatt aacataaatt tagttaataa taatcaatta ccaacagaaa
    41161 tttttactaa gacctttttt gatacaatgg ttttattggg aggatgtggc tctttactat
    41221 gtttagtaat tgcaattttt cttagtgaaa aacgtataaa tgtaagaaag ttagctaaaa
    41281 ttgcttcaat accagctctt tttaatatca atgagatgct agttttcggg ataccaattg
    41341 tttttaacta tattatgttt gtaccttttg taattactcc tataatctta accgttacaa
    41401 gttatgtggc tatgtctaca ggtattgtac catgtacagt ttcagctgta gaatggacct
    41461 cacctatatt tttaagtgga tatatgtcta caggttcaat aagaggtagt ttgttgcaag
    41521 tatttaattt atgtgttggt gttatgattt atattccgtt tataaaaatg tcgcaaaata
    41581 gatatacata tatgtttaaa aataatatag aaaatttaac aaacttatta aaaaggtatg
    41641 aattaacagg tgaacagcct aatcttctaa accataatgg taaaataggt tcaatttcaa
    41701 aaatgttggc atcagacttg aagtttgcta tgaaaagagg agaaatagag cttttttatc
    41761 aaccacaagt caattataat ggaaatgttg tgggagcaga gggattactt agatggaaac
    41821 attcaatagg agggttcatt tatccaccac tagttataat tttggctaaa gaagaagggt
    41881 tcttagatga gcttggagag tatattatta ataaagcatg tattgattta aagaagagtg
    41941 aaaagctatt gagaaatcct gttaagttct ctgtaaatat atctccagac caacttgatg
    42001 accctaagtt accagagcaa ataaaaaata tcattagtag taatgatata aatcctaata
    42061 tgttaggtat agaaataaca gagcaaattg cattatcagg ttcacaaata attattgaac
    42121 gtatcaatgc gatacataac ttgggaataa agttaattat ggatgatttt gggatggggc
    42181 atagctctct tatatatctt caaaataata actttgatat agttaaattg gatggttcat
    42241 tagttaaaga gatacttaca aataaaagaa gtagtgaaat tataatgact attgtaaatc
    42301 tttcaaaaaa tttagatttt gatatagttg ttgagtatgt tgaaaatata gaccaaaggg
    42361 ataagttata tggtttagga tgtgaaatat atcaaggata ttattatagc aaagcaatac
    42421 cttttgaaga gtttgtagaa tatgcaaatc aagaaagaga gatataattt tataagagct
    42481 tgtaaattct agtaatgcat agatttgtaa taaaattaag tttaggagaa atatatgaat
    42541 ataagtatag tagtagtagg taaaattaaa gaaaaatatt taaaattagg tattgatgag
    42601 tttaagaaaa ggctttctaa gtattgtaag ttagaaatta tagagttgga tgatgaaaaa
    42661 gctccagaga atttgagtgt taaggaaatg gaaattataa aagacaaaga aggaaagaaa
    42721 attttaggga agataaagca taatagttat gttatagcct tggctattga tggtaagggt
    42781 ttatcctctg aagagcttgc taagactatg agtgatttag cagttagagg taatagtagt
    42841 ctctgtttta taataggagg ttctttaggg ctttctgatg aggttttagg aagggctgat
    42901 tataggttat ccttttctaa gatgactttt cctcatcaga tgatgagatt gatattgtta
    42961 gaacagattt atagggctta taggataaat aatggagaac cgtatcataa ataaatgata
    43021 cggtttttgt taatttgaca cttaataata ttaaaataac aaaaaagtat tgaaagtata
    43081 ttgcatttaa ggtagaatta aataaaatat tataacatta taatgtttat atggttgtta
    43141 ataaaaattt ggaggaaaat atatgataaa aataatgttg gcctgttcag caggaatgtc
    43201 tacaagttta ttagttacta aaatggaaag tgcagctaaa gaaaatggta tagagtcgca
    43261 aatttgggca ataccagaat caactataca aaatgaaata gaaaaatgtg atgtattatt
    43321 actaggacca caagttagat atgtattacc taaggctcaa gaaattgcta agccatataa
    43381 tataccagta gaagttataa atatgatgca ctatggaaca gttaatggag aagctgtttt
    43441 aaatagagcg atagaactta acaacactaa gtaatataaa ttataatgta tttttaatat
    43501 ataaattggg agaatcaaag gatggatgaa aagactatag aaatatcttt taatataata
    43561 ggttatgcag gtgaaggtaa aggattagct tttgaagcaa taaaagaggc taagaatgga
    43621 aatatagaaa aagcaaaaga attattaaaa gagtctaggg aagttataaa taaagcacat
    43681 agatatcaaa cagaattaat acaaaacgaa gctagtggaa acaggactga aataagtgtc
    43741 atacttatac atgcacagga ccacttaatg aatggcatga attttcaaca gttagctgag
    43801 gaaatcgttg atttacattt gaaattacaa gataaataaa aattaaagat aataaattaa
    43861 gagagttctt aaaagataca ttatgtgtag cacactaatt gttagattat tgattcaata
    43921 tttagtgagt aatacattag gattattttg aggactcttt ttatatttag tttaaagtta
    43981 ataaatattt attattaata atataagatt atatgttata atattataac atataattta
    44041 agaggtgaat catgttaaga aatcaaccat tatatataca agttgtagat atattaaagc
    44101 aaaaaatatt aaataacgaa tataaaattg atgaaaatat tccaacagaa agggagttag
    44161 agcaagagtt tggagtcagt aaaattacta ttagaaaagc tattgagata ttagaatcag
    44221 aaggatatgt agagaaaaaa agtggaaaag ggacaaaagt aataagtaat agccttttta
    44281 ataagctatc aaaagcagag tctttttcaa gtatactttt atctaaaggt cataagttat
    44341 ctaaagagac tatttcaatt gaaaaaataa taaatgatac aaattcacaa gtatttagta
    44401 tatttggaga aaaatgtagc aagattacta gaatgtatta tttagatagt aagccatata
    44461 tattttttac tcattatatt ccttttgatg aaaatctaac tgttgaaagt tttaataatg
    44521 aaagttcttt atatatgtac ttatatagga gaaatataaa tataactaga tttgaagatg
    44581 aattttttat agaagaatca gaactagagg tatcaaaggc tttaaatcta gctaaagaca
    44641 tattattagg aagaaaaaga aagacctatg atgagaatga taatatagtt gagctatcat
    44701 ttgcaagata taatacagaa gtacaaaact atgtaattaa atacgaaata taataagaat
    44761 cctgagttta ttgaaatgat aatatgctca ggatttttgt ttagttgtaa aaataaaaaa
    44821 tattataaca ttataatgtt tacaaccaaa cattataatg ttataatatt tttaataaac
    44881 aagtgatatt atcaattaca taaaaattag tttatgttat gaaatcgtta ttgcattata
    44941 aataaaggat aatatcaata atacaggggg ataactatgg aaaaattcgc aagtttttta
    45001 gagcgcaaga ttatgcctgt agcagcgaag ataagtaatc agagacatat gagagctgta
    45061 agagcaggta taatagcaac attaccacta acaatagtgg ggtcattttt cacaatatta
    45121 ttaaacatac ctattgatgc ttatatggat tttatagctc catttaaagc aatattagat
    45181 ataccattta gatatactgt aggattttta tctctatatg ctacttttgg gatagcatca
    45241 tcacttgcta aaagctacga tttagataca acaggtgttg gtatgcttgc agttatggca
    45301 tttttagttt ctacaatagt tccaactcaa gtagttgaac cagtaggtgg agttatagaa
    45361 gctggtagat ggatggatat ggcaaaactt aattcgccat cattatttgg agctatcgta
    45421 acatcattaa ttacagttga aatatatcgt tttatgaagg aaaagaatat tacaattaag
    45481 atgccagcag gagttccagt agaggtttca aattcattta ctgcattatt accgacagca
    45541 gtaattttaa tgttattctg ggtaataaga catattctag gatttgatat aagtgttgca
    45601 ctaagcgcta tgttatcacc tttaaaagga ttcttatcag gaaatagttt aggtggtggg
    45661 ttactgacag tattcctaat aactttattc tgggtacttg gaatacatgg tccagctata
    45721 atgggaccag taataagacc tatatgggac atgtctatag cagaaaatat aactgcattc
    45781 acagaaggag tatcagctca tgcaatgcca aacatattta cagaacaatt tttacagtgg
    45841 tatgtatgga tagggggagc aggggctact cttgcattag ttgtaatgtt tatgttttct
    45901 aagtcacaat atctaaaaag tttaggtaga ttaggatttt taccagggtt attcaacata
    45961 aatgaaccta taatatttgg agcaccaata gttatgaatc caatactagg gataccattt
    46021 ataattggac ctataataat gacaattcta tcatatgttc ttactattac aggggttata
    46081 cctatgatgg tagcaagatt accatttgca atgccagcac caatagcagc agtaatgagt
    46141 actaactgga gtatactagc aggtatctta gtattaatca actttgtgat atcatttgct
    46201 gtttattatc cattcttcaa agtttttgaa aaacaacaat tacaaagaga acaagaagaa
    46261 atgagtgaaa atttataaaa atacatagat atagaggttt tgtactatat aaaatataga
    46321 aaaagcttat aagttattaa gggtgaaggt aggaggaatg acctttgcct ttaattttat
    46381 atattttaaa taatgtaatt tgaggaggag aaagatggaa agaagattag gaatatcagt
    46441 atatccagag catagtaaca tggaagatga taaggcatat ataaaaatgg cttcaaaata
    46501 cggatttaaa agaatattta tgtgcatgct ttcaattaca agacctaagg aagaagttaa
    46561 aaaacatttt aaagagatta taacctatgc aagagaatta ggatttgaag taatccttga
    46621 tatagctcca aacatatttg atatattaca aatatcttat gatgatttaa cattttttag
    46681 tgagttaaat gctagtggaa taaggcttga ctatggatat gatggaagta aagaagctat
    46741 gatttctttc aatccatatg atttaaaagt ggaactaaat atgagtaatg atgtagcata
    46801 cttggataat ataattactt acaagccaaa tgtaaataat atatatggtt gtcacaattt
    46861 ttatcctcaa aggggtacag gcttgccata tgattttttt gtaagatgtt caaatagatt
    46921 taaaaagtat ggaataagaa ctgctgcttt tgttaattca aaatacgcaa caatagggcc
    46981 ttggaatata aatgatgggc tgtgtacgct tgaaatgcat agaaacttag atattgctac
    47041 agcaaccaag cacttattcg caacaggatt gattgatgat gtaattgtag ggaatgcata
    47101 tgcaagtgaa gatgaattaa aaagaatgtc tgaagtaaat aaatatcaag ttgtatttaa
    47161 tgtagtaact gaagaaaaaa cttctgaagt agaaaagaca ataatggcaa atgagcaaca
    47221 ctttagaaga ggggatataa ccaaccaagt agcaaggtct acagaagtaa gaaaaaaata
    47281 tgctaaagaa aatttcccta agcatgataa tacgaccatc tttaaaagag gagatgtagt
    47341 gattggtaat gatgactttg gaaagtataa aggggaatta cagttgatac ttcaagattg
    47401 tgcagatgaa agaaaaaata aggtgggtag aataaaggaa gacgaaataa tacttttaga
    47461 ctttattgaa ccttggagta aatttaaact tattttagag ggagagtaaa gtgaagatag
    47521 acatattaat aaaatgtgga aaaacagtag ataaaagtat aattgatata gctatattag
    47581 acgataaaat aatagaagta aaaaatcaga tagatgattc taagtatcag gcaaatcaaa
    47641 ttataaacct agatggagaa aaatttatta gtgctggttg gatagatata catacgcact
    47701 gctacgaaag actagacctt tataaggatt atccagatga ggttggaata aaaagcggtg
    47761 ttactactgt agtagatgca ggaacaacag gagctttaga tataggtaaa ttttatgaag
    47821 atactaaaaa atataaaaca aatgtatatg ctttaataaa tattgctaag cagggaataa
    47881 catctcaaga tgaactttca agtatgatga atattgatga atatgaactt agaagagcag
    47941 ttaaaaaata caaagatttt gttgttggaa tcaaagctag aatgagtaaa agtgttgtaa
    48001 tttcaaatga tgttgaacct ttaaaagtag ctaaaagaat aaaaaatgaa cttaatctac
    48061 ctatgatggt tcactttggg agttctcctc caacgataga ggatatattt gactatatgg
    48121 aaaaaggaga tatattaacc catatatata atggaaagcc aaatggaata cttagaggca
    48181 atgaggttaa aaaagaaatc atagaagcta gagaaagagg tataatcctt gatgtaggtc
    48241 atggaaccga aagttttagt atggatattg caatgaaatc taaggatgca ggaatttttc
    48301 cagacactat aagtacagat atatatatta aaaatagaat aaatggtcca gtgtataatc
    48361 tatctacaac aatggaaaag tttatatata tgggctattc acttgaagat ataatagata
    48421 aagttactaa aaatgcagca gatgcaataa gtttaaaaaa taaaggttta ataaaagaag
    48481 gatatgatgc tgatttaact atatttgatg tggttaatga agaaaaagaa cttcaagatt
    48541 ctttgaataa gtcagtaatc acaagtacgt ctataaagcc aagagcagta gtagtcaatg
    48601 gagaatattt aaatataggt aagtctatag gagagaatga gtagaaggga gaaactattg
    48661 tatgagtagt atatttgaaa aatactctgt aagacgtgtt ataaatgctt caggcaaaat
    48721 gactatacta ggagtatcaa cagtagataa ggaagttatt gaagcaatag gtgaagggtg
    48781 taaaaatttc tttataatag aagaacttat aaataaaaca ggtgaatata tagcaaattt
    48841 attagggtgt gaaagtgcac taatagtttc tagtgcatct gctgggattg cacagagtgt
    48901 gggagcaatg atagcaaaag atgatatgag tctagtttac aatgtaaata atcctaagaa
    48961 acaagttaaa agggaaataa taattccaaa gggacataat gttgattatg gagttccagt
    49021 agaagtaatg ataagacttg gaggaggaga agttgttgag gccggttatg ctaatatgtg
    49081 taacaaggaa catataataa gagagataaa tgaaaataca gcagcaatta tgtatattaa
    49141 atctcatcat tgtgttcaaa agagtatgct aagtgtatta gatgcagtac aagttgctaa
    49201 agaatataat ttacctttga tagtagatgc agcagcagaa gaagatttaa atgtatatta
    49261 tgaattggga gcagacctag tgatatacag tggtgctaag gctatagagg gtcctagctc
    49321 agggttagta ataggtaaga aaaaatacat agataatgta aagcttcaat caaagggtat
    49381 aggaagagct atgaaaatag gaaaagaaaa tattgtaggt ctaacatgtg caattgagag
    49441 atatataagt aatgataaag taactttaaa agaaatggag gataagctaa atccatttat
    49501 agacaagata aatactataa agggagtgag tgctagtata actagggatt cagcaggtag
    49561 agaaatactt cgtggagaaa ttgactttaa tgaagatata ataaataagt caacacaaca
    49621 aattataaat gaacttagaa gtggagagat agctatatac actagagact ataaggctaa
    49681 tgaaggcaag attgagattg atataagaag tgtaactgga agtgatttag atactattta
    49741 taataggata aaaaatatag tagaaagtta gagtgtagag taagtgacat tataatgatt
    49801 taataaatta aattttacca atggagggga taaatatgaa ttgtatacca caatattata
    49861 aaaatagagt ttgtttaaat gttcttgcag gaagcataaa aaatgctaaa gaagtatatg
    49921 atgcttgtga cgggcatgta ttgataggaa tactatcaaa gaattatagc actgtagaag
    49981 cagctattga agatatgaaa aaatactcaa aggaaataga taatgcaata tctgtggggc
    50041 ttggagctgg agacccaaat cagtcaaata tggtatctca gatttctaaa gaacttcagc
    50101 cacaacatat aaatcaagtg tttacaggtg tggctacgtc tagagcattg cttggtcaaa
    50161 atgatagtat aataaatgga ttagtttctc caactggtaa accaggattt gtaaaaatat
    50221 ccacaggtcc attaagttca aaagaaaaag atggtatagt tcctatagaa acagcaatag
    50281 ctatgatgaa agatatggga ggaagctcaa ttaagttttt tccaatggga gggcttaaaa
    50341 caaaggatga atatatagaa gttgcaaaag cttgtgcaaa gcataatttt tatttagaac
    50401 caacaggggg tatagactta gataacttca aagagatagt tcaaattgca cttgatgctg
    50461 gtgtagaaaa agttatacct catgtttata catcaataat tgataaagaa acaggtgaaa
    50521 cgaaagtgga agatatagga gtactttata aaataatgaa agaattattg ggatagattt
    50581 atgaatatat tagcttttgg tgaaataatg atgagacttt cagtacctga ctacaagttc
    50641 ttaacacaaa ccaatgaact aaattatata actacaggca cgggattaaa tatattatct
    50701 ggacttaaaa actttggata caatacctat atgttgacaa agttgcctaa taataatgta
    50761 ggtaaagcat caagtgcaaa tataagaaag cttggagtaa aagatgactt tatcacgtat
    50821 ggagggaatc atataggagt gtattttctt gaaaatgggt atggagaaag accttctgaa
    50881 gtgacttatt tgaataggtt aaatagttca ttttgtgaga gtaaattaca agattatgat
    50941 attgataaat gtttagaaga aatggatata gtccatattt gtggtattgc tatgcaactt
    51001 acagattcag ttaaagagat tgctttagaa ctagctagaa aatcttatga aaaggggata
    51061 aaagtattct ttgattttaa ttttagacaa tcattaaatc cagaaaggac ttatgatgat
    51121 ttagtaaatg attataagaa aatactacca tattgtcatg ggctgtttgc tagttataga
    51181 gatataaata agatacttaa tatagatagt ctaaatataa aatctagttc agaattgatt
    51241 aaaaaagagt atgatataga tattgtagct ggaactatga gaagatttga tgatatgaat
    51301 aatagattta taaagggata tatatcaaat gatgatggtt attttgaaag tagggagtat
    51361 aagctagaaa tatatgacag ggttggtgcc ggagatggat atgtagcagg aattatatat
    51421 tcatatttgg aaaatatgaa gagtgaagat atagttgagt ttggggttgc tagttcggtg
    51481 ttagctcata caacttatgg ggataattct ttagtaagca caggtcatgt acttagactg
    51541 atgaataatg aaaaaataga tatattgaga taatacattt gtatataata aaatttttat
    51601 cttttttaga cacatttttg acttgcttat atttgttaaa cagagttatt tttaatgatt
    51661 ataaatatta aattaaaagt taaataataa aatagtagaa aatagcatta actatagaaa
    51721 aaatggttaa tgctattttt atcgttaata ttgattaatt ttatattata gaaatgaact
    51781 tttctaatgc tggatttgta ttttttttat tccatacaac tacaacttca attctatttt
    51841 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51901 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    51961 tatttgaacg ttgtaccaca ttaggtacaa accctgcttc acgatatcta ttctgtatca
    52021 ttgacttttg tccatactca tcccctgttt cctttacaaa aataaattta tcatgttgta
    52081 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52141 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52201 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52261 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaaatatt
    52321 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52381 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52441 cctcagataa aaaaactttt ccagctggtg ttagttcaac atgacgttta cttcttgtaa
    52501 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52561 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52621 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52681 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52741 aaaatatttg tataaaaata tatgttttaa gtatatcttt acattagatt ttataaattt
    52801 attaataaga ataacttata tattccaatg aaaacgatat tggatttaag taagtttgtc
    52861 tcataaaatg aaagtatatc aattgataat actttaaata agaagggaga aaaaatggaa
    52921 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
    52981 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
    53041 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
    53101 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
    53161 aaagagtatc ctacaacaac aactcccaaa attcaaatag cagatgattt aatgatagta
    53221 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
    53281 aagtgtgaag caggaattag aatatatagt ttaaaagaag accctttaaa tcctaagttt
    53341 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
    53401 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
    53461 gtcatagata taataaatcc tactaatcca gtggaaatag gtaaatggtg gagaccagac
    53521 caatatgcaa atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
    53581 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
    53641 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
    53701 ttaggtgaat tgccatttat gcctgcattt tctagtagac ttgcaggtgc aagaactcat
    53761 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
    53821 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
    53881 gatgttagtg acccaacaaa accaacatta attgctcaat ttccatatcc tgaagttcca
    53941 aaagatttcc cttatcctaa ctttaatgtt gcgggattag gaaaaccagg gccatttggc
    54001 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
    54061 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
    54121 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
    54181 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
    54241 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
    54301 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
    54361 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
    54421 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
    54481 gcgtgtgtta aaactttatt atctgggaaa ggtagtatgt gtgtattaaa agatgatttt
    54541 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
    54601 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
    54661 agaggaaata acattatagg aacaaaagtt gcagaagcta taggaggaaa aattccagat
    54721 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
    54781 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
    54841 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
    54901 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
    54961 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gccaggtact
    55021 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
    55081 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
    55141 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
    55201 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
    55261 caagctggag tttcattagg ataataatta aaatactaag actctttttt aaggtatgtt
    55321 agatgttata ggaggcttat tgtatgaatg aaaagtcaag ttttaaacaa attgaaatta
    55381 ttttagcagt tatttttttg gtacttacat attacaaggt tagattaact ccagcattta
    55441 ttgttatatt tataccacta tatttaagtt attttgcagt atctatgatt gtaaatcaaa
    55501 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
    55561 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
    55621 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
    55681 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
    55741 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
    55801 aatcaaaacc tagaattatt tttaacactt agtatattag gtttttttgc agttgtaatt
    55861 gcatccttat ttgaaaaaat aattttccta ccatcatggc tttgtggttg ggctataggt
    55921 ctaactatta tgacaggtga aaatattatt aatttacaag atatttgtat tcaaattgct
    55981 gtagctatgg cagtaggagt atggtacgtg ggtgcaggtg tagatttgtt tacaatatat
    56041 attttgagta aagataggaa aaagggaaat tgaaagcatg tgaatatact ttgattatag
    56101 gagaatggct atgggttttg aatttaaaat aatgagaagt ttaatatatg taggacttgc
    56161 caaggaagaa tatagaccta agctaatgga ctggttatat cgtcaccata ttccagatag
    56221 tattagcact tttggaccat attgtactaa atatgccttt tatcaagcat atcctacacc
    56281 aaatgaaggt gagcgttttg gtgcacgtaa gatgcaacta acagaacatt attggcttgt
    56341 agatgaacat atgcctgaga tggcaaatag aattatgaca gaatatatgc ctatggatgt
    56401 tctacgttgg caagggtgta taccagatgt agaaaataaa agggttcatg aaaatgcaga
    56461 aagtggagat gcaggacgtg cagtaggtgg agataatgga tgtccaccat ttatatttgc
    56521 ctttgttcca ataaactggg aagaagactt tagaggaaaa ggacgtactg tacaagatgg
    56581 accaaactat cgttggcaat ttatgattaa gtatccagat ggtatctcta aagaagaagg
    56641 agaaaaatgg ttctatgatg aggtagtgcc atactttaca aactgttgct atgttaatcg
    56701 ttttgtcagt agtaaaataa tgattaatta tggagcaact gcttttgacc gtgtatcaga
    56761 actatggttt gaaggggaag aagaatggta taaagctgtg gttgaagaga caaagtcgtt
    56821 tattaaaaaa ccagaatggg cacaagaaga ggagttccca tatttaaaac cacaattcaa
    56881 tatcgcatca gtattcttag gtgatatagc aactatggat gcatactcac agtatcgtgg
    56941 atatatacca atgagataaa aattatagtt tgtgataagt agaggaggca tttactctta
    57001 ctggtagaag aagacttatt gtatccttaa aagtaggtga atatatagtt ttatatttaa
    57061 atttatataa aactaagact aataaatatg aaacaataga attagaataa tttgataata
    57121 tttgttaaaa gagcatttag aatgtgttta aagattaaga tattcctttt atggtagcag
    57181 atagttgaaa taataaaact gttatcatga aaggaatatt tttatgtata ggaaattttt
    57241 aaaaataaaa gttaaatatg tttaagaaca tcttaaagtg aaagaatcat tgttagaaat
    57301 atgtagacaa tatagtcttt ataacgtatc atattaacaa gtttacactt ggattatgaa
    57361 ataaaaagag taattccaaa ggttgtaaat actatgctga ctttgatact tcaaagttta
    57421 ggtgattttg taattgttaa tagaaatacg tttaatgagc ataaagaatt tatctaataa
    57481 taggagaata taatgtatac aattggacaa gtatctaaat ttttaggaat atctagagat
    57541 acactaaaat tttatgagga caaaggatta gtaaatccta agaaaaatga tgaaaatgga
    57601 tatagaatat ataatcaggt tgatatctat gatattgcaa caataaactt ttatagagaa
    57661 attgatatag aaattaaaaa gattcaagaa ataagaaaaa gtaagagtat aaacaattta
    57721 gaactcttac tagaagagaa ggaacaaatc atactaaaag agatagaata taaaaagctt
    57781 ctcttaaaaa aggttcagtt agtcaaagaa gattgtgaca aaataaaaac atattttgga
    57841 aaatacacaa taaaagaaat gaagcctttg gaagtaaaag gtaaaataaa acatgttact
    57901 aactatgagg aatatgaagc cttaaaagag aatacaaaca acttaaaaaa agcagttaca
    57961 ttaactgatt taagaagagt aataaatttt aatgaagaag gtataataga agaaaaattt
    58021 ataattgtaa gggaagtaga tgactacaac aaaatcatag aaggtgaagt tctatctcat
    58081 cccaattgta tttatactat tattgaagat ggcagatggt caactggagg agaaaatatt
    58141 gataataaag tggaagacag attaagagaa atagcaatag aaaatgaatg tgaattactt
    58201 ggaatagtct atgttaatat attgcttact acttatgaag aagggattga gcgtatattt
    58261 ttagagattt atgcacctat aaaataatat aaaagcattg acctgggact tactcatagg
    58321 tttaaactca tgatataagc agttataact tatatagcaa ttaaatatta atttgaaatg
    58381 aggattactt atgagaacag agaaatcatt aaaaaggcct atagccagtt ttattattct
    58441 aacaaatata atttttttac cacttttttt attggtagta gtgactataa tgttaaaact
    58501 tccaacagtg atatctaaca ttgcgttgtg tatatcagcg tggtcatcca cttttgcatt
    58561 tatgatatta tttaaaaaaa tttatccagg taagaaattt ttagtacacg taaaagatag
    58621 atttagaaat aaacttaaat tttcaacagt atctataata attagtatac aagtcctaat
    58681 agctgtagta gttatattta tattagcatc taaaaatcat ggaataatgc ctaactttac
    58741 agtttcatca tggggagcat ttatatattt atttttaaaa aatttatttg caggatcatt
    58801 aggtgaggaa ttaggttgga gaggatttgt tcaaaatcat cttcagaaaa gacattctcc
    58861 tttaaaagca tccataatag taggtttttg gtgggggatg tggcatttac caatatggtt
    58921 tacaacagga ttcaaaggac ttgatttaat aaaatatatt atatttttta tgattgcaat
    58981 tatatcaatc agtattataa tgacaacttt ttataatcta aataaaactt tgataatacc
    59041 tattgtaata catcaactat ttaatttttt aatagggata attaatggag atttaataga
    59101 gcttataaat tactatgcaa tgttatattt aatatttgca attgcattaa tagttataaa
    59161 tccaggaaat gtattatata aaaatattag agagtaatat atatctttat ggtaagatat
    59221 aaagatatta ttagaagaac atccaaggag gaataaacat ggttttactg attgttgatg
    59281 cacaaaagtt aataacaaac gagaggttat ataaatttaa tgaatttgta gtaaatgtag
    59341 aaaatttaat tgatacagct agaaaaaata atatagaagt aatatatgta cgtcatgatg
    59401 atggagttga aaatgaattg actaaaggta aaaatggctt tgaaatatac gaaaaattta
    59461 aaccatgtaa tggagaaaaa atatttgata aaaaagtaaa tagtgctttt aaagaaactg
    59521 gtctattaga atacctaaca aataatggtg aaaaagatat tattattgct ggtctccaaa
    59581 cagattattg tattgatgcc actataaaat gtggatttga acatggattt aatataatag
    59641 ttccagctta ttcaaataca actgttgaca atttttttat gtcagcagaa caatcatata
    59701 aatactacaa tgaatttata tggaatgaaa gatatgcgaa gtgtatttca ttagaggaaa
    59761 cacttagaaa aatgaaataa aaatagagta tgaattgtat atgtacataa gttgatttca
    59821 taaaaaagga tgttaaattc caagttaaaa attagattaa atatattata ttatagataa
    59881 ggcacatcaa ttttatttta aattaatgtg ctttttaatt gtagtaattt atttataatc
    59941 aatatgattt ttattattta aatcaatttt tcttgagttt ctttgtctaa agaaactttt
    60001 tttatttata aaatctacag ttatattata gtatctattt tagagaagtt taatttttgt
    60061 atcaatctaa aacaaaagtt atacttaaat gaaattgaaa acgataaaat tctcaaataa
    60121 aataagtatt gtggaggtga tgaaagaatc caatgataat atgaagacta atcttaataa
    60181 gaaagtaggg gttaatggta gtggacaaag aaattgtatc aattctccag attttattaa
    60241 atagcacatt gattacaact aatggattac aagaggagtc taattcatca aagagacaaa
    60301 ttacttatag aataaataaa atcaatgaca tgcttaaagt aaaaaatgta ccacttatat
    60361 gtttaagagc tgataaagat attatcgtta gaaaagaaac aaaaaaagca attaaagaaa
    60421 ttttagaaaa aaactattct aagaatactt attattttag taagacagaa agacttttat
    60481 atatgtattt aatgcttttt attaacatgg atgatatttc aattaatcat tttataaatt
    60541 ctataaaagt aagtaggtct actgtaaatc ttgatttcaa ggatttgata ccagagcttg
    60601 aaaagaagaa cattaaagtt aagaacaatc gtataaatgg atattacata gttggcaatg
    60661 agatggaaat taggagagtg cttattaaaa atattataga aactttgtct aatgagaaaa
    60721 gttcaagagt atttgatatt tttattaaag aatataattt agatagtttt gaagaggcta
    60781 aaaaaattat attaaagcta ataaaaaaat atgaaattac ttttgttgaa gatagattag
    60841 tagaatttat atatatattt atctttctaa aggcaaggat gtattcaact agaatagtta
    60901 ctcaggaaaa tgtagatatt cctaatattt cagttatgag ttcaatgaag gagtataaat
    60961 ttgctaagga attattagaa gtatatggga gtgaagataa gataaagcct cacaatataa
    61021 tgtatattag tgcttggatt ttaggtatat cagtaggaaa ttatgaagaa gatacagaag
    61081 ataaggatgt aatttcaaaa attgtaaata aaatgatgac aaagtttgga tatttaagcg
    61141 gagtttacta tataagccaa gaaaaaatat ttaaacaatt atattctcat tttagacctg
    61201 cctactatag acttttattt aaattaccta tttataatcc gatttgtgaa aaagtaaaag
    61261 aagaatataa attagtttat agaattgtaa gtaatgcaat gaaagagttt tgcggattat
    61321 ttggagaaga aattcaggaa gaagagttag catatcttac aatgcacttt gcaacacttt
    61381 tttcaaataa aaaagaattt gatggtttta gaaaaagaat agcattaata gtttgttcaa
    61441 atggcgttgg tagttcagct attttacatg cagaacttac aaacttattt cctgacttac
    61501 actttatacc aatgtcggaa tcagttggat tagaaaatat aagtgaacct gtagatattg
    61561 tatttactac taattataac gcagagggtt taaaaacaga tgtaccagta attaaagtaa
    61621 gccctgttat gaccacaaaa gaaaaatata agattacacg tgaagtgtat attcaattag
    61681 gtgatatttt tttaagacaa cctaaaatag atgaggttat ggatataata aaaaaatatg
    61741 caaatgtaac ctctgaaagt attctttcaa gtgagctatt agcttatttt acacagatag
    61801 agaattttac atctaaggaa ggagaggggc ctatgctaag tgagattact aatgaaacac
    61861 tagtaaaaat aaaggtaaaa gctaagaatt gggaggaagc tattagacag tcagcttctg
    61921 tattagttga aaataacaaa gtaactgaag ggtatgtaga tgctatgatt aactcagcaa
    61981 aagcatctgg accatacata gtaattacaa aacatgttgc acttccacat gctagaccag
    62041 aagctgggtc aaaagagatt gcaataggca ttgctacttt agatagtcct attgagtttg
    62101 ggaataacga aaacgaccct gtaaaatatg tattttgtct aagtgcaata gacaataata
    62161 gccatttacg tgctatgtct gagctagttg aactattgga agaagatgag ttttttaagg
    62221 ttttagatag tgctaaagaa gctaaagaaa ttattgattt tataaaaaca cacgaattat
    62281 aaaggaggat ttaattatgt ataaaatttt aatcgcatgt agagcaggag ttggctcaag
    62341 tttaatgtta aaaataaaga ctcaacaaat tattaaggaa aataactttc caattgaagt
    62401 agaacatggt tcattggatt cactaaatgg attcaatgga gatgctgtag aaacattaat
    62461 tgatgttgct gaagaattaa aaaataaaaa tttgaagttt gatataattg ggattcacaa
    62521 tattgttgac cgtaatgaaa taaagacagc actagaaaaa ttccttgctt caaaagaggg
    62581 ataaatttaa gatgataagg aggaatattt atggagttta ttattagttt gctgagtaca
    62641 cctgctgttt tgcttggcgt agttgcatgt ataggattga ttcttcaaaa gaaatcagct
    62701 gttgaagtat ttacaggctc ttcaaaaaca cttatcggtt ttttgatatt tggtattggt
    62761 gcctctgcta tgacaggagc acttcaaaat ttcaataaac tgttccaaca tggctttgga
    62821 attactggtg ttatagcttc accagaagca gctacagcac ttgcacaagg tacatatggt
    62881 tttgcagttt catgtacatt aattcttgga ttcatattaa atttagtatt tgcacgtatt
    62941 acaaagttta agaatatttt ctttacaaca ggtcatagtt tgttcttttc ttgtgtatta
    63001 gttttaatta tgaaagcaca tggatttgat aatacaatca ctattttaat aggtggaact
    63061 atcttagggt ttatgtctgc tgcactacct caattctgtc aaccatttat gcgtgaatta
    63121 acaggtggcg acgaacaagc tattggtcac tttaacatga ttggttatgg tttatcaggt
    63181 tatattgggc gtttattttc aaaacataaa gatgatacaa cagagactat agaatttcct
    63241 aagtggttat caatattcag agacttctta atgggtctat ccatagttat gttaatctta
    63301 ttttatattg caacattaaa agctggtcaa gcatttactc aagaaattgc tggttcaaca
    63361 cattggttgg tttttccgct tatagaggca tttacatttg tggcaggtat gtcaatattg
    63421 atgtcaggag ttcgtatgtt cttagctgaa ataactgcag catttgtaat catttcagaa
    63481 aagtatattc caggttcacg tcctgcgcta gatgttccaa ccgtatttcc atatgcacca
    63541 aatgcagtta ttattgggtt tatatcagca tatgctgctg gtctattagc aattgctatt
    63601 atggcatcat ttcctagtat atttcctgta gtaattattc cagctgctca catatgtttc
    63661 ttctcaggag gtacagctgc aatatttggc aatacttcag gaggatggag aggggctatt
    63721 gctgggtcat ttgttgtggg gttattactt gcatttttac cagttatact atatccagtt
    63781 tatgggactc ttggtataga aggagcaact ttccctaata ttgattacaa cgtagttggt
    63841 tcgatattac ataatatttt acaatttata aaacctattt agactaaatt atataaaagt
    63901 atagtcatag ttactatgat ttcaaggagg atacatctta tggaaaatac attatctgaa
    63961 tttttagata atatatctaa tgaattgtca gaatttattg cctcaataga tgagacatct
    64021 ataaataaag catgcgaatt aattcttgaa gctgaaaaaa atcacggacg tgtacatgtg
    64081 actggtattg gcaaaccagg acatgtatcg ggatatattt catcattgct ttcatctact
    64141 ggaacgtcag catatatatt gcatggtaca gaagcagtac atgggtcaag tggtcaagta
    64201 gttgaaggag atgttgttat tgcaatatca aatagtggtg aaactcaaga gttaaaagca
    64261 actcttaaga ctctaaaagt aaatggagca aagataattg gtgtaagtgg taatgagagt
    64321 tcatttttaa aaaatatttc tgatatattc ctgtttgcag gtgttaagca ggaaggagat
    64381 tgtctcaata aagctcctcg tgcttcaatt ttagctgaaa caataatatt acaatcatta
    64441 agtgttgtat tacaatatgc taaaggtctt aatacacagg agtatttgaa gtggcatcca
    64501 gcaggaagtc ttggaaagtc tattagagag ggaatataat tttatgaaat ataaagggat
    64561 tatatgtgca atgataacac catttgatga aaatcaaaat attaattcac aagcaacatg
    64621 tcaattaatt gattatttga ttgaaaaggg aatttatgga ttatttattt tgggcactaa
    64681 tggtgaatgt catgtattaa cagatgatga aaaagttgaa tttgcaaaga ttgttattaa
    64741 tcatacaaat aatcgtgtac ctgtttttgt tggaacaggg ggtaattcaa ctcgtgaagt
    64801 tgtagattta tctaagagaa tggaagcgat tggagcaagt gcattatcta ttattacacc
    64861 atactttgtt acaccaacac aacaggaatt aatcttacat tacaaaacta ttgctaattc
    64921 agttaattta cctattataa tgtataatat gccaggtaag actggaatta atattgaacc
    64981 agagtcagta cgtgaattat caaagataaa gaatattgtt ggaattaagg atagtagtgg
    65041 taaacttgac aacatgaaag cttatattga agcaacgaag aatgagaatt ttagtgtgtt
    65101 ttcaggctca gattcactta ttctggatag tctaaaagct ggtagccaag gagctgttgc
    65161 agcaacaaca aacttcctca cagagattgg tatctctatc tatgaaaact ttatgaaagg
    65221 ggatttggaa gaagctcaaa aatctcaaag tagtatagaa gaacttcgac gtatactgaa
    65281 acttggaaca attccatctg ttatgaagaa aactattgtc ttgaatggaa ttaatgttgg
    65341 aactgcacga cttcctgtaa ctgaaccaac tggggaagta cttgaagaaa ttaaacgagt
    65401 agttgaaaat tataggacaa tacttaataa aagtagtgag aatagataat ataaataggg
    65461 aaattacact ggtatataat taatatatat attataaaac atgactaaaa ttaatgaata
    65521 agtaaagtgg tttgatgagt atagaggtga taatatgaaa aagatgaata taaccaaaag
    65581 aatgtcagaa tgtggggctc ttgcaattgt aagagaagaa aatcttaacc gtgcatgtga
    65641 aattgctgaa ggatgtataa aaggtggtat aacagttata gaaatgagtt ataccctaaa
    65701 taatgctgga gaaataattc aagggcttaa taaaaagtat ggtgaaatac tttgtgtggg
    65761 agcaggaaca gttttggata gtgaaacagc tcgtcatgct atattacatg gagcccaatt
    65821 tattattgca ccaaattata atgaaggtgt agcgaagatt tgcaatagat atcagattcc
    65881 ttatgctcca ggatgtacat cattgactga ggcagtagac gcactaagtt taggagcaac
    65941 ttttataaaa gctttcccaa tatcagattt ctatggacct aaactagtaa aggtatttaa
    66001 gacaccaata cctgatatgc cagttttagc aagtggaggg cttaatattg aaaacctaca
    66061 tatatggtta gaaaatggag ttgatgtatg tggatttggt ggtttattaa ctaaaggttc
    66121 tgttgaagat attgctaaaa atgctaacaa gattcgtgaa atcattaaaa atgtaagaaa
    66181 aatttaacat agggctgtat catagtatct tataattact atgatacagc ctttttaata
    66241 ttattcaaat ttaaaacagt ttacatttct attatattac atataatttc agtaatttta
    66301 tcttaatttt tctatctgga aaaaaatgat ttaaaactta agttatgata taatttcatt
    66361 aaaagaaggc gatttttagt aaatatgaga aaataatgtg tttttagggg aaaagttaaa
    66421 tacagattta agtgaacaag aatcgataat taagatatta aatggtgaat agggcatcaa
    66481 aaaaattact aaattaaaag ttttagaggg agaatcttag caagtgtgtg tgaaaattca
    66541 atttcaaaca aaaataattg taaatgaaat caatttatac acgttgaaga ttcactttta
    66601 tcattatatt aaaaagcaga gtgaattaaa agtgagtcta aagataggag gataattaaa
    66661 acaatgaaag aagacattag gttacatcga atgtgtaaaa aagaacaagc aacaaagaat
    66721 gaagttatat cagaggatat tctaaaatct atgagtattg gggtcagtaa acatttttta
    66781 gatgattcat ttacaataat atggggaaat acatcattct atcaattatt aggatataca
    66841 gaagaagaat ttttagttaa attttctagt ttaaaagatt attatgaaca gaatctttat
    66901 cattttgaat ctatgaaaaa tcatttttta gaggcttatc atcatggcga aaagagtata
    66961 gaatatcaaa tttgcatttc agcaaataat ggagaccctg tttgggtaat aatgactggt
    67021 atattaattg aatcacaaga taatgagaaa tcaatggtct atatattgta ttcaaaaatt
    67081 aatgagttaa aaataaagca agaagaacta tctgatatgg aaaaagagag tacagagaat
    67141 tttaaatgga tgatgtcaga gtatgctggg aatgtttata ttagcgatat ggatacatat
    67201 gaattactat acttaaataa gcactcatgc aatactttac aggcatcagc aaatgaactt
    67261 attggaagaa aatgttatga ggtaattcaa ggtagaactt cgccatgtcc gttttgtaca
    67321 aattcgtatt taaaaaaaga tgacatatat gaatgggaat tttataataa aaaactgaaa
    67381 cgaacattta taattaaaga ccgtatgttg aattggcaag ggcgaagagt tcgtattgaa
    67441 ttatcttatg atatgtatag tgctgaatat aaattagcaa aaaaagatca ggaaagagaa
    67501 tcaattttga aaactattcc agcaggaatg gttagattag atgcaagaga ttatcttact
    67561 atactttggt gtaatgatat atttttgaat atgattgaat atacaaagga acagtttgca
    67621 gaggaactta ataatcaatg tggatattta ttttctgaag attataaacg tagtaagaaa
    67681 ctggcacaag atttgaagga gtctggggat aatgttgtat ttgaagcaaa aatatataca
    67741 cgttcaaggg aagaacgtat atggactgtt acattgtgtt atatcagtgg ggaagacagt
    67801 tgggatggaa taccatcttt ttatagtata ggtattgaca taacaaagga aagaaaacag
    67861 attgagaaat tacagcatat atcagagaaa gatgcattaa caggaattta taatcgtgct
    67921 gagactgaaa ggcagattaa gaaatatttt gaaaaaaatt taaatgttat gggtgcactg
    67981 tttatgattg atacagataa ttttaaacag attaatgata cagaagggca tatgattgga
    68041 gatatagtac ttactgagat ggcatcaggt atgaaaaaaa taatgcgtga cagtgatgtt
    68101 gttggaagaa taggtggaga tgagtttaca atttttatga agaatatttc ctcagttaaa
    68161 gatgcagaaa agaaagcaga agaactattg tatatgttcc gtcatctatt tcagaaagaa
    68221 aaaagttttc taaaagtaac ttgtagcata ggaatagcta tatatccaaa ggatggtact
    68281 acattcaagg aaatatatgc aagagctgat aaagccctgt atcaagcaaa aaatatggga
    68341 aaaaataatt atgtaatcta caaccaagat tattttaaag aattagaaga atttgattgt
    68401 tcttctcttg gtacagttat tgattcagaa aaacaatacg ctgaatatcc tgataatctt
    68461 acacgttata tattccgtat gctttatcag actgatgata tagaccaggc gattaatatg
    68521 atacttgagg ttgttgggaa acagtttgat gtaagtcgtg tttatatttt tgaaaataca
    68581 gaagatggta gatatacttc taatacttat gaatggtgta atgaaggaat ctcttctcaa
    68641 atggcttatt tgcaaaacag caattatcaa gactatgagg attatgaaaa gctttttgag
    68701 gatgaattag ttttttattg tcgagatatc catacattaa gtcctaaact agaagaattg
    68761 ttttctagtc aaggtatcca ctctacactt caatgtgcat ataaagaaga tagagttttc
    68821 agtggatttg ttggatttga tgaatgcaca ggactacgtc tatggtcaca agaggaaata
    68881 agtacattat cattgatttc tcaaattata tcaatgtttt tacaacgtaa aaaaaataat
    68941 aaacttaatc aacagattca acaataccag aatattttag ataatattga ccagtgtatt
    69001 tgtgtgatta ataaagaaaa taatttttta ttatatgtaa ataaaaaatt taaaaataca
    69061 tattctaaat tccaaattgg acaaccttat tatcttgatt ctatagatat tcataagata
    69121 tccattatat ggaataaaaa agaagcatac ctatgtacta gcacgattta atggtaaagt
    69181 aaagtttata ttggacttaa attaaatttc gttttaaata agatattatt ttagttgctt
    69241 actgaaaaga ctagaatcta tattaatgaa ttatactaat tttataccac tattaaaata
    69301 ttgtttgcta aaaataaaaa atagccttaa aacagttgat tataaaaaat taactgcttt
    69361 aaggctattt ttatctttgt gacgaaatag acacttgcca tttagaatgt gacaatatag
    69421 tagttgttta gaaaaggcac aaagcattat actttaacta aagtaaaatg tattttgctt
    69481 ttagaataaa aacaattagg agggagctat aattgaaaaa gataatatat gtattattta
    69541 caattgaact attaatagta tctttattag gtctaaatat agttaaagat aatgaaatta
    69601 ataatatttt atataacgat acaacaagta tatctgtaat gtttaaagat tataaaaagt
    69661 taaataaaaa ttatagtaag tggattaaag atattgcaga tgaaaataat gtggctatat
    69721 caaagtatgt ttttaataat aatgaaaaat tatctatata tacgactgat acaagtttga
    69781 ataacaatat aaagttaaaa tcaggaaaat ttcctaatac taattcaaat gaatttatta
    69841 gtaatataaa cactaataat agtaatcaag taggtaaatt ctcaactatg agtaaagaca
    69901 tatcaatttt aattagggat tttggtaaaa taagtgaagt tggagaatca ggaatcttat
    69961 atatttcaac tcaaaatgaa gatacaataa ataaaatatt aagagaacta aatgtagaca
    70021 gaagtatttt tgtagcaaga ttacatgata gatatgttaa ctcaaattta tacttaaatc
    70081 cttcaataat tagagattta atactaatta tcctttgttt cttggctaca ataatacatt
    70141 attcaattag tgtgagtaga gaggcttcta ttttaagatt aaatggatac tctaagctag
    70201 atataattgc tagagttata aaaaatatac ttagaattat gattttatca tctgtcacag
    70261 cattattaat atatataatc tatattttaa aattaaatat aggtgttaga gcatgcttat
    70321 actttgctat gttttcgatt atatgtattt tgataaatat tttgatatct atattaatag
    70381 ttggttttaa tagtagaagt tctaaatatg tgctatctct aaatggaaaa aaatcttatg
    70441 gttttgtaaa tataatgcat ttcacattga aaattgtatt tgtatcattt cttatattat
    70501 ccataaataa ctgtatttta aattataata tgctacaaac tcagttagga aatctatctg
    70561 agtgggataa agcaaaaaat gtttataatc ttactcttaa agatacaggc gaacaatctt
    70621 taggaaagga agaagtaatt aaaaatgcca agattaaagg tttctataaa aatttggtag
    70681 aagaaaaaga tgctttctta atagattcaa ctaattttga aaaacttgaa gatggaagtt
    70741 acatgtatga aataaattca aagggtaaaa acccagaagt aagtcaatat ggtaaaaata
    70801 taaaaataaa taaaaactat cttaaagtta accctattta ctcaaatggc aaagaaatat
    70861 ttaatctaat cgattatgac gataagacta taaatttatt agttcctaaa aagttacaag
    70921 tacatgaaaa tgagattaaa aaagagttta gagaattatt ttattttgaa aaaatagaag
    70981 tagaaaatat gtacaataaa gagttaaata gagagttaaa taaaacaaag aagtccgatt
    71041 taaatgttaa tataatatat gtagataata atcaaagcta ttttacttat aactcccttg
    71101 ttatggatga caataggaat ctcattgatg acccaatagc aatagttgac atagataata
    71161 ttgatgattc tttttatctt tcttatatat ctagatgtgt atattttaat tctaagaaat
    71221 tagatgccct tgcagatata agtagcatta ttgaagctca aggtgtagaa gcgcatatcc
    71281 aaagtttaca tgctgtttat aatgagtatg gtttagaaat aaataaacta gaaaaatatt
    71341 tgaatagtga aatttttact ataataataa tagcaatatc aaacttaatg attacctaca
    71401 atatagtagc tagctactat gaaagaaata aatataagtt atatattaaa aaaatatttg
    71461 gttactcaac tacagctaga agtatgttgt taatagtctc attaatatta acaaacatta
    71521 ttccaatagg cattatatca acaagagtag atttaagtaa taatattatc ttatttggac
    71581 ttctaatatt agttatagaa gtaattgtat ctattgtatt agacaagatt atctcaaata
    71641 gttcatttaa taaaattata aaaggagaac attaaaaaga tgatttcatt aataaatata
    71701 tctaaaaaat ttaaagataa atctataata gacaaattta acctagaaat ttctaagggc
    71761 gagtttgtag cgataacagg aaatagtggt tcaggtaaga caactttact taatataatg
    71821 ggattattag aaaaacctaa ttctggtgat attataataa ataatataaa aaatccaaat
    71881 agtaaacaga taagaatttt acaaagggat ttttatggct atctatttca aaattatgct
    71941 ttaattgaga atgaaaatgt tgaaaataac ttgaaaattg ctttaaagta tcaaaaaaat
    72001 atcaatcata atgaaaaaat aaattacgca cttgagtctg taggtcttag aaaatacaat
    72061 aaaaaaaaga tatacgaatt gagtggagga gaacaacaaa gggtagcatt agcaagagta
    72121 atattaaaag aatgtgaagt aatatttgca gatgaaccta caggtaattt agataaaaaa
    72181 aatagagaca tagtatttga tatacttaaa agtctaaata agaatggtaa aactatcgta
    72241 tttgtaactc atgatttaga tttatcggag caggcagata gagttataaa attgtaataa
    72301 tataaactat aataaatata agaaagagat tacagctaaa ttaagtttaa taataatacg
    72361 aatagaattt aaacattaag taaattagta gtttgtaaat ataagtataa tatgatatca
    72421 taaaggctca aaagaaattt tatataataa gaaatctaaa aataacaaat ggaggacata
    72481 taaagatgga aaatttacca aattgcccga aatgtaattc agaatatact tatgaagatg
    72541 gaactctttt agtttgtcca gaatgctctc atgagtgggc attaggattg ggagctgaag
    72601 aagattcaaa tattatcaaa gatgtaaatg gaaatgtatt aaatgatgga gattctgtta
    72661 cagtaattag agaccttaaa gtaaaaggaa gttcatcatc tataaagatt ggaacaaaag
    72721 tgaaaaatat ccgtttaatc catgactcat cagatggaca tgatatagaa tgtaagatag
    72781 atggttttgg agctatgaag ctaaagtctt ctgttgttaa aaaagcgtaa tattataaat
    72841 aagctgtagt gtttattgtt tttcagtaca gtataaaacc ataagaatct attaagtaat
    72901 aaaagtttgt ttatttatct ttatattttc ttatggtttt attaaatata aattttattg
    72961 aacaccaaaa tattctctcc tctacaaata agccaatctt atttaaacta atatactttg
    73021 tactatttat actgaaattg taaatttaaa tgctatgtta tttaggtttg tatagttaat
    73081 ttatatattt ctgattttat aaattgtatt tattattata tcttgattct aaaatcatta
    73141 aaaatttcaa cttctataaa gatttacaat ttatcaaata cattatgagt atataaacaa
    73201 tttattatta cagtgctaca tttaaaataa gtcttagtat aaaggtattg ttacaagtat
    73261 aaagaaactt atgtatgcta ttggaataaa taatacctta aattataatt aagaataaga
    73321 actgttaaat aagggacttg attatcattt agttatagat aatataatat taaaaataaa
    73381 gtttaagagg agaaaaaata tgatagatat acataaaaat atatatgata ataagttatt
    73441 tgaggagcta aagattgact gtaagaagtg ctttggactt tgctgtgtag ctctttattt
    73501 ttctgcttca gatggatttc ctatcaataa ggaatcagga aaaccctgta ttaatttaca
    73561 accagatttt aaatgttctg tacataatag tcttattaag cgaggtttta aagggtgtac
    73621 tgcatatgat tgttttggtt caggtcaaaa agtagcacaa gtaacatata aggggataga
    73681 ctggatgcaa tcaccagaac taacaaatca aatgtctgaa gtgtttttaa ttatgagaca
    73741 attacatgaa atgttatggt atcttaaaga agcatctgta ttaaatatat cagatacaat
    73801 caaatcaaaa attgatttaa taattgaaga aactgaaaag ataactaata tgggtccaga
    73861 acagataatt aatttggata tcatatcaca tagaacaaag gttaatttat tattatcaca
    73921 agcgagtgag tctatgatgg gaaaggttaa atcatttatc aagacttcta ctcttaaaaa
    73981 catgaaaaaa ttaagtaaaa atatagattt aataggagca gaccttagaa aaataaatct
    74041 aataggagca gatttaagag gtagatttct aatagcagca aatttaagaa atacagattt
    74101 gagtggggca aatttaatag gagcagactt aagagattgt gatattagag gtgctaatct
    74161 tgaaaatagt atatttttaa cacaattaca agtaaatact gcaaaaggag attctagtac
    74221 gaagttacca gcttcactaa tacgccctaa atactgggaa aaataattaa attaatacat
    74281 aattttacaa aatatgaact tttttgactt taattcatat tatacattag agattcttta
    74341 caagaatttc ttaatataat atagggagag atataaaaat gagttatcaa aattataaat
    74401 gtgaaagaga ttatgtgtat gatgattata aatacaataa gtgcaacaag tgctataatg
    74461 attatgagga aatgacacat gttcatgaat attcagaaag tgttaagtta gcagaagaat
    74521 gtgaggatag acataaccac cgtgcagcag gggttactgg tgaagttata ccaataaatg
    74581 gaggtacaaa tcatgttcat aaaataaatg ataatgtaga tttccttgac catttccata
    74641 agatatgtgt tacaacaggg ccagcaatta gaatacctgg aactgataaa cacattcact
    74701 taatatgtgg agaaactact gttaatgatg gtcattgtca taaattcctt tttactactc
    74761 aaatagaagc acctttagta taagctgatg tgaattattg tagtgaattt gctagattat
    74821 agtataatat ataatagata atatttaaac ttaatgagga ctatgatatt taaccttcgt
    74881 agtcctttct aagttttgta taaaaaagtg ttatgcaatt aaaagtgata tagggaggaa
    74941 atttttatga aatctggaat aattcttgaa atgaaacaaa taaattcaac agaatatgac
    75001 gaaatgttgc aagtctggga atcatcagta agagcaacgc atgatttttt aacagaaaaa
    75061 gatattgaat ctttgatacc attagttgag attggactta aagaagtaga aaatatagtt
    75121 tgtataaaag ataatgatat aataaagggc tttataggaa tagataaaga taaaattgag
    75181 atgctcttta tagaagataa atatagagga aatggtattg gtaaaaaatt aataaaatat
    75241 gctatagata aatacaatgt aaaatatgtt gatgtaaatg agcagaataa aaaagctgta
    75301 ggattttata ttcacttagg atttaaagtg tttgataggt ctgaaataga tggacaaggg
    75361 aacccatttc caatactaca tatgaaatta atttaaagtt atacttccca cttattcgca
    75421 aaaatattta cttaaatttt tatacaaata aaagtcgtga cagtcttagg acacgacttt
    75481 tttactacac tattaaattg aaatttatct gttctatatt atgattatta gatgtagttt
    75541 ttttatttta actatgtact taaataccaa gtatacccat acctatcaag tacttcagca
    75601 gcgccatcat tttgatgtgg tggtgggtgt ggtaaaaatt cttgtacaag tgtaccacct
    75661 tcggcaagtt ttacagatat ttcttctatc gcttctcgag atggtagaac aacacaaatc
    75721 gccatgccag aaattacaac aggcttgtct gtatccgcac ccattataca accttcgtat
    75781 tttgtgaaag taacacttgc gtgcataata ggattgtttg gattgctgtc taatcgcgca
    75841 aatatagttt ttcctcctcc gaaagcctca gcataaaaat taaatgcttt ttcacaagta
    75901 ccattaaata ttaaatatgg agttagcatg accaatatat cctccttgat atagaataat
    75961 ttatagttgc acctgataat ctaaattatg atttagtaaa ttgtatcata gggaaacctt
    76021 gaagtatata attttttatt attatattat acaaatttat taatttaaaa aaatgttata
    76081 tgtacggaag gtttacagga taaaatataa taaaaagatt aaggtgacat tataaaaaat
    76141 gtatattgac attgacgcta cgtaattgta tatactttgt cttaaaggag atggtaaata
    76201 tgaatattat agaaattcgc tcagataaaa tatacaagaa gataatggat gcaccaataa
    76261 acaaaaaaga agatatatac agatatgaat taatgaagcc ttttgaattt aagtggaagt
    76321 gtatgaatgt tccaatagtt gctagacaga aaggtggata tgatgtaatt atagcaagtg
    76381 aaatgttagg ggttttatcg cctaaggata ttgatgaaaa gcaaaaaaag aatataaatg
    76441 tgttatctgc tgataaaatt tggggcactt gcaaagaaac catagaaaac tctataaatg
    76501 cttttataaa agaagggtat gatttaaaca ttaaggacta taaatattca atattattgg
    76561 cgaatccaaa tagtccttat acaatattaa gtgatggata ctggggtgat ggtgggattc
    76621 ctggatatat atttctatca ttggttccta atgaatatac tatcaataga ttaccagtat
    76681 taatagcaca tgaatgtaat cacaatatta gatttcagtt tatagagtgg aataataata
    76741 taacattaga agaaatgatg ataaatgaag gtcttgcaga aaattttgca acatggatgt
    76801 ttggagagga aatgttagga ccttgggtca gtagaacaga tatcgaaaca ttaaatactt
    76861 atataaagcc aataataaaa agtgctttaa aagaaactgg atttcaaaat ataacatctt
    76921 atctttatgg tgatgatata gctaaaatgc aaggatattt tccagtaggg ttgccttatt
    76981 gtgcaggata tgcttgtgga tattatatga ttaagtatta tttagaaaag acaaataaat
    77041 caataatcga agcgacttta ttgccttata gtgagataat cgaagcagta aaagagtttt
    77101 gggaataaat tttataaatt gcataatttg aaataaatta tgcaattatt ttatttttaa
    77161 gaa
//
LOCUS       DAYAOY010000005        73281 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYAOY010000005 DAYAOY010000000
VERSION     DAYAOY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.005"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            24..683
                     /gene="cprR"
                     /locus_tag="ACGI6B_000391"
     CDS             24..683
                     /gene="cprR"
                     /locus_tag="ACGI6B_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HFG9749860.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            894..1952
                     /locus_tag="ACGI6B_000392"
     CDS             894..1952
                     /locus_tag="ACGI6B_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFG9749861.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2203..4347
                     /locus_tag="ACGI6B_000393"
     CDS             2203..4347
                     /locus_tag="ACGI6B_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2617..2619,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFG9749862.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4396..5229
                     /gene="fdhD"
                     /locus_tag="ACGI6B_000394"
     CDS             4396..5229
                     /gene="fdhD"
                     /locus_tag="ACGI6B_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFG9749863.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5240..5413
                     /locus_tag="ACGI6B_000395"
     CDS             5240..5413
                     /locus_tag="ACGI6B_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749864.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5471..6022
                     /locus_tag="ACGI6B_000396"
     CDS             5471..6022
                     /locus_tag="ACGI6B_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9749865.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6047..7423
                     /locus_tag="ACGI6B_000397"
     CDS             6047..7423
                     /locus_tag="ACGI6B_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HFG9749866.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFSVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7423..7938
                     /locus_tag="ACGI6B_000398"
     CDS             7423..7938
                     /locus_tag="ACGI6B_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9749867.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8016..9299)
                     /locus_tag="ACGI6B_000399"
     CDS             complement(8016..9299)
                     /locus_tag="ACGI6B_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HFG9749868.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9487..9909
                     /locus_tag="ACGI6B_000400"
     CDS             9487..9909
                     /locus_tag="ACGI6B_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HFG9749869.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10011..10949
                     /locus_tag="ACGI6B_000401"
     CDS             10011..10949
                     /locus_tag="ACGI6B_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFG9749870.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11135..13159
                     /gene="ligA"
                     /locus_tag="ACGI6B_000402"
     CDS             11135..13159
                     /gene="ligA"
                     /locus_tag="ACGI6B_000402"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFG9749871.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13319..14668
                     /gene="rph"
                     /locus_tag="ACGI6B_000403"
     CDS             13319..14668
                     /gene="rph"
                     /locus_tag="ACGI6B_000403"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFG9749872.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14668..15138
                     /locus_tag="ACGI6B_000404"
     CDS             14668..15138
                     /locus_tag="ACGI6B_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9749873.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15332..16618
                     /gene="tig"
                     /locus_tag="ACGI6B_000405"
     CDS             15332..16618
                     /gene="tig"
                     /locus_tag="ACGI6B_000405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFG9749874.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16768..17352
                     /gene="clpP"
                     /locus_tag="ACGI6B_000406"
     CDS             16768..17352
                     /gene="clpP"
                     /locus_tag="ACGI6B_000406"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFG9749875.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17371..18621
                     /gene="clpX"
                     /locus_tag="ACGI6B_000407"
     CDS             17371..18621
                     /gene="clpX"
                     /locus_tag="ACGI6B_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFG9749876.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18692..19219)
                     /locus_tag="ACGI6B_000408"
     CDS             complement(18692..19219)
                     /locus_tag="ACGI6B_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9749877.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19207..19719)
                     /locus_tag="ACGI6B_000409"
     CDS             complement(19207..19719)
                     /locus_tag="ACGI6B_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9749878.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19899..22262
                     /gene="lon"
                     /locus_tag="ACGI6B_000410"
     CDS             19899..22262
                     /gene="lon"
                     /locus_tag="ACGI6B_000410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFG9749879.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22252..22863
                     /gene="yihA"
                     /locus_tag="ACGI6B_000411"
     CDS             22252..22863
                     /gene="yihA"
                     /locus_tag="ACGI6B_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFG9749880.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23128..24498)
                     /locus_tag="ACGI6B_000412"
     CDS             complement(23128..24498)
                     /locus_tag="ACGI6B_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9749881.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGSVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24673..24788)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25076..26155
                     /locus_tag="ACGI6B_000413"
     CDS             25076..26155
                     /locus_tag="ACGI6B_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9749882.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26546..27226
                     /locus_tag="ACGI6B_000414"
     CDS             26546..27226
                     /locus_tag="ACGI6B_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9749883.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27219..28598
                     /locus_tag="ACGI6B_000415"
     CDS             27219..28598
                     /locus_tag="ACGI6B_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFG9749884.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28769..29854
                     /locus_tag="ACGI6B_000416"
     CDS             28769..29854
                     /locus_tag="ACGI6B_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFG9749885.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29908..30267
                     /locus_tag="ACGI6B_000417"
     CDS             29908..30267
                     /locus_tag="ACGI6B_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9749886.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30320..31192
                     /locus_tag="ACGI6B_000418"
     CDS             30320..31192
                     /locus_tag="ACGI6B_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFG9749887.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31310..31636
                     /locus_tag="ACGI6B_000419"
     CDS             31310..31636
                     /locus_tag="ACGI6B_000419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749888.1"
                     /translation="MYVKSINELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEI
                     KKIYETIGINSITKISIDKNKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYY
                     RFRIKS"
     gene            31651..32235
                     /locus_tag="ACGI6B_000420"
     CDS             31651..32235
                     /locus_tag="ACGI6B_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749889.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQENSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32488..33525
                     /locus_tag="ACGI6B_000421"
     CDS             32488..33525
                     /locus_tag="ACGI6B_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFG9749890.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33606..33971
                     /locus_tag="ACGI6B_000422"
     CDS             33606..33971
                     /locus_tag="ACGI6B_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749891.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            33976..34293
                     /locus_tag="ACGI6B_000423"
     CDS             33976..34293
                     /locus_tag="ACGI6B_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749892.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34341..35945
                     /locus_tag="ACGI6B_000424"
     CDS             34341..35945
                     /locus_tag="ACGI6B_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9749893.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36069..37697
                     /locus_tag="ACGI6B_000425"
     CDS             36069..37697
                     /locus_tag="ACGI6B_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFG9749894.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            37962..39311
                     /locus_tag="ACGI6B_000426"
     CDS             37962..39311
                     /locus_tag="ACGI6B_000426"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFG9749895.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39467..40546
                     /locus_tag="ACGI6B_000427"
     CDS             39467..40546
                     /locus_tag="ACGI6B_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFG9749896.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            40891..41799
                     /locus_tag="ACGI6B_000428"
     CDS             40891..41799
                     /locus_tag="ACGI6B_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HFG9749897.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            41804..44173
                     /gene="hypD"
                     /locus_tag="ACGI6B_000429"
     CDS             41804..44173
                     /gene="hypD"
                     /locus_tag="ACGI6B_000429"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HFG9749898.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            44498..45301
                     /gene="proC"
                     /locus_tag="ACGI6B_000430"
     CDS             44498..45301
                     /gene="proC"
                     /locus_tag="ACGI6B_000430"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFG9749899.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            45899..48655
                     /locus_tag="ACGI6B_000431"
     CDS             45899..48655
                     /locus_tag="ACGI6B_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9749900.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            48787..49266
                     /locus_tag="ACGI6B_000432"
     CDS             48787..49266
                     /locus_tag="ACGI6B_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9749901.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            49282..49704
                     /locus_tag="ACGI6B_000433"
     CDS             49282..49704
                     /locus_tag="ACGI6B_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9749902.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            49744..50535
                     /locus_tag="ACGI6B_000434"
     CDS             49744..50535
                     /locus_tag="ACGI6B_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9749903.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            50528..51328
                     /locus_tag="ACGI6B_000435"
     CDS             50528..51328
                     /locus_tag="ACGI6B_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9749904.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            51376..52431
                     /locus_tag="ACGI6B_000436"
     CDS             51376..52431
                     /locus_tag="ACGI6B_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9749905.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(52610..54556)
                     /gene="feoB"
                     /locus_tag="ACGI6B_000437"
     CDS             complement(52610..54556)
                     /gene="feoB"
                     /locus_tag="ACGI6B_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9749906.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(54574..54792)
                     /locus_tag="ACGI6B_000438"
     CDS             complement(54574..54792)
                     /locus_tag="ACGI6B_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9749907.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            54998..55507
                     /locus_tag="ACGI6B_000439"
     CDS             54998..55507
                     /locus_tag="ACGI6B_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFG9749908.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            55585..55746
                     /locus_tag="ACGI6B_000440"
     CDS             55585..55746
                     /locus_tag="ACGI6B_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HFG9749909.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            55897..56562
                     /locus_tag="ACGI6B_000441"
     CDS             55897..56562
                     /locus_tag="ACGI6B_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFG9749910.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            56628..58412
                     /gene="pepF"
                     /locus_tag="ACGI6B_000442"
     CDS             56628..58412
                     /gene="pepF"
                     /locus_tag="ACGI6B_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFG9749911.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            58664..59506
                     /locus_tag="ACGI6B_000443"
     CDS             58664..59506
                     /locus_tag="ACGI6B_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9749912.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      59739..59822
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            60402..61079
                     /locus_tag="ACGI6B_000444"
     CDS             60402..61079
                     /locus_tag="ACGI6B_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9749913.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            61151..63166
                     /locus_tag="ACGI6B_000445"
     CDS             61151..63166
                     /locus_tag="ACGI6B_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9749914.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            63216..63896
                     /locus_tag="ACGI6B_000446"
     CDS             63216..63896
                     /locus_tag="ACGI6B_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9749915.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(64047..65165)
                     /locus_tag="ACGI6B_000447"
     CDS             complement(64047..65165)
                     /locus_tag="ACGI6B_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9749916.1"
                     /translation="MNIKSAFIRKRGEKFHVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFITPNKTTLVERCYKYIMTNEKNWSPYTVINRKSWVKNYIEPFF
                     KDTILIDVNPSLLQIFIDKNFDGSAPSTAKVRYNFLSSVLKEAYRLKEISENPCDFVK
                     LPAQNVTYEIEIYNREETLLLIEKLKDSLIEIPILLMLLLGLRIGEVAGLRWSDVNLD
                     NSIININQILIYANSKITFKEPKTAKSKRALSVPKELVEKLKIEKLKQNKMKLQGTLI
                     NENNLVCLNTNFNAWIPTALSKTFHNFIKRNNLRNIRVHDLRHTNASLLLLGGTNMKV
                     VSERLGHTDIKITMNRYSHVLEEMDKEASDNLSKLLFK"
     gene            complement(65679..67343)
                     /locus_tag="ACGI6B_000448"
     CDS             complement(65679..67343)
                     /locus_tag="ACGI6B_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFG9749917.1"
                     /translation="MNNENEQLKSDLLINDEKIKNLNFKIPWYYSLWTISILILSTFS
                     TYSISFIVAIIFLFKRNKIMKKHKDSISILLSDVEKINNKYILLNDEIKMKEKHFEDL
                     CESNENKLKELSNLLDKKKVEIDKFDSENQDKFKLIEELKIEKERLDNLIKDKNILKD
                     NINTLNSHLEELKNEREELRDINTTLKNKKEELKRLSEELIQTEDEVLLQSFGLYNPK
                     YDFENSDEYMEKLKEIREMQKLLIRNKTGVKYSDSWTVDGSVQKGRTMTNQNIKTALK
                     LFNSECDIAMSKVSFKNIDSIEKRIRKAFTDTNKLNTSNKVSIKENYLNLKIDELYLY
                     YEYLQMKEEEKEEQRALREQMKEEALVQKEIENQKRKLKKEELQFKNELLRLKSTIPE
                     DENDKLEWEQKINSIEEKLALLSKDLDDVLNREQNTRAGHVYIISNIGSFGENIYKIG
                     VTRRLDPTERINELSSASVPFKYDIHATIFSEDAPKLESALHKAFDNKRVNKVNNRKE
                     FFKVTLDEIRTEVEKNFDKTVEYTKLAEAQEYRQTLKIQELNNKLA"
     gene            complement(67614..69062)
                     /locus_tag="ACGI6B_000449"
     CDS             complement(67614..69062)
                     /locus_tag="ACGI6B_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HFG9749918.1"
                     /translation="MSILNLFRKNKNKNNVDCNEKSNESKQSYNDSTTLSLECNSNSP
                     HNIEVKNEINKVLNVSCNDTTNLYELEKQAREKSDALYDIHTNSINKFNPQNNILETD
                     TKPLTSIEKSFLKYIIGENIYEPYIATYWTYEYNINYSYLISKFFNMDYLKISNYIED
                     LTKLTVSELKEILKSNNIKSTGKKAELIERIEKEISCKDLSNFFNSSNKYYALTDKGK
                     ELLKDVRKSVTKNTDLEDQCLELIYIDKYEEAYDLICKYESSKNIQRGININWENHKI
                     TPMKIESYKAIKELDINLKDTLLDNIIKSSYILCDMLGNNSKTSILVKRLVGEKIDNI
                     EINNAINSINDVIYNHSTITYNANDSINNKNLHYMDIVKNNKSIPFEVNNKNYNFKIN
                     ENEIYFFNILKIKMYENELKNNFVFDRMSDGAFNVFDTSDNFIGKVKLQGRKKWIMYT
                     KNEFGSEHIYGELIHLIDGIDAWIKYSKEYLK"
     gene            69559..69699
                     /locus_tag="ACGI6B_000450"
     CDS             69559..69699
                     /locus_tag="ACGI6B_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749919.1"
                     /translation="MEKSKKLLIDTGDTLNKLRENNSEKLEKYIELLKEYYKQENKKE
                     RE"
     gene            complement(69714..70079)
                     /locus_tag="ACGI6B_000451"
     CDS             complement(69714..70079)
                     /locus_tag="ACGI6B_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749920.1"
                     /translation="MIGYRIKELRKEKDITQKELATFLGLTPKMISFYEKEERFPPHD
                     IILKLSDFFNVSTDYLLGKVNVKNIDNLSELELIENLNFSDDIKEALKLISELSPSSQ
                     EKMFKIAKVFLEEELNEKK"
     gene            70224..70460
                     /locus_tag="ACGI6B_000452"
     CDS             70224..70460
                     /locus_tag="ACGI6B_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9749921.1"
                     /translation="MNKLKDFRIQKGLSRNQLGAELGLTSRYIAFLENGERVPSLNTA
                     VKIAAYFNTSIEYIFLKNNCTKSTFKEKGAEKYE"
     gene            70453..71244
                     /locus_tag="ACGI6B_000453"
     CDS             70453..71244
                     /locus_tag="ACGI6B_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFG9749922.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            71314..71643
                     /locus_tag="ACGI6B_000454"
     CDS             71314..71643
                     /locus_tag="ACGI6B_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9749923.1"
                     /translation="MSVALQFIDTKDLVQELMRRDDTTDIIKMFLDREGIKRMELMTI
                     EEFCEYLKISDVTARNMAREAMITKDFIALKIGRKYMIDRISFEEFIMKNAMKDKDVM
                     KKRKGVI"
     gene            71745..71972
                     /locus_tag="ACGI6B_000455"
     CDS             71745..71972
                     /locus_tag="ACGI6B_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749924.1"
                     /translation="MKILKVKFPTKILKAGKAIRVTCARFGFESDCIITQSQEFELTV
                     VYFDKELDDLVQSSITIDDAISYDYCIEMLN"
     gene            71985..72302
                     /locus_tag="ACGI6B_000456"
     CDS             71985..72302
                     /locus_tag="ACGI6B_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749925.1"
                     /translation="MKSLIIVRNAVEQQLNRANLEINKNEQLYTKLRKKEERNISEEI
                     ELSNALREKSVNERLKIFAESLLEIIDTQIEIKEYEENEDCKIFQLILEELERDRPID
                     VQI"
     gene            72386..72895
                     /locus_tag="ACGI6B_000457"
     CDS             72386..72895
                     /locus_tag="ACGI6B_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFG9749926.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRMQELKKIKKNALDRLKSNIKECMELLGTKKVETFL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNSIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            72905..>73281
                     /locus_tag="ACGI6B_000458"
     CDS             72905..>73281
                     /locus_tag="ACGI6B_000458"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFG9749927.1"
                     /translation="MEINNIYIKLMNVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDAT"
BASE COUNT        29376 a         7955 c        12636 g        23314 t
ORIGIN      
        1 aacggataaa tggaggagtt attatgtcta agatattagt agtagatgat gagttagata
       61 tgttaaagtt gattgaaaat gtgttgaaaa gagatggaca tgaagtaaag gtaattagtg
      121 atgtagaaaa agctatgaac atggatttta attattttaa tctaataata ttagatgtta
      181 tgatgcctaa gatggatggt tttgaagtat gtaaaactat aagaaataag gttgattgtc
      241 caatactgtt tttgacagca aaaaatatgg aaagtgatat tatgtacggt cttggtatag
      301 gtgcagatga ctatataacc aaaccttttg gtgtaggaga attaagggct aggataactg
      361 ctcatttaag gcgagaaagt agagaaaaaa agaatttatt aactatctca aatgttcaat
      421 ttaacttttt aggcaaagaa gtttcaattg atggtaaaaa aattaatttt actaaaagtg
      481 aatatctaat atgtgaattt ctagcaaaaa ataagggaca agtattttca aaagaaagaa
      541 tatatgagtc ggtatatggt tttgatggag aaagtgatat aagtactatc acagagcata
      601 taaaaaatat acgaagcaaa ttaaaacaat atgatattta tacaatagaa actgtatggg
      661 ggatagggta taaatgggtg taataagtat aagtgttgtt tagtgaaaat attatatata
      721 tctacaaaat gtttgtaaaa tattaatcta tatattaaaa tttaatttat agattaatat
      781 tttttgtaaa tgacaaaatt gtaaggtatt ttgttataaa atattaagat tcatagtgta
      841 tactaataat atagtattta gtataaatct tgagcaaagg atggttattt ctaatggata
      901 gactaaagaa tattagaaag agtttactac agtatacatt tttatttttg ttaataggta
      961 taacaattta cctagtgttt aaaacattgg atattaaaat gctatcaaat gttatggtta
     1021 tggtagataa gaagttttta ttcataggtg ctttggcaat aatgtgtcac ataatgcttg
     1081 aaggagtagt aatgaagata attatagaaa gcactcacaa agttaatatt agatttattg
     1141 gttttaagct agccattatg ggattttatt ataatttaat aactcctttt gcttcaggaa
     1201 gccaaccagt acagatatat gtacttaaaa aatgtaaaat gccacttagt aaggcaagtg
     1261 cagttgttac caataagtct attatcttcc aaatagtagt tactttttat tgtacaatac
     1321 ttgttttaat gaattttgct atgttagaaa aacaaatgaa aatgattatg cctctagtgt
     1381 ttctagggat agccattaat tcatttgtct taattatgat aatattagtt attttaaatc
     1441 ctgaaaaaat aaaatatttt atgagaatta ctataaagca tttatcaaaa attaagtttt
     1501 taaaattttt agataataag attgaatcca tagaaagttt tatagatgat tatagtaaag
     1561 caataaattt ttttgcaaag aataaaaagg tattaatttc aactataata gtaactttta
     1621 ttcaattaag tgcttacttt agtgtttcat tttggatata taaagcattt aatttacaag
     1681 gttatagcta tatttatata ctgacattac aagcattttt atatatggca atatctccaa
     1741 taccaacacc tgggaatgta ggagctaatg aattagcctt tttcactata ttcaaatcag
     1801 tattccctca acctttgatg ggatatgcag tatttttata tggtggattt atgtactatt
     1861 tgattctgat tggaagtgga atatttactg taattactca ctatagaatg aaaaacagaa
     1921 ttggcagaaa tattgtttta agtgaaaact aaataagttt aaagaatata tattgagtat
     1981 attaaaaaat atgtcataag aactagaata ttttaattaa atggttgaaa tataaagatt
     2041 attatgacat attttttttg aaatttaaga ggaattgtga gaaaattgtt gaatttaata
     2101 gatagtattg caaaatattt atgattttgc atattataaa aacactcatt atctttaaga
     2161 taaattttac ttatcagggg ataaatctaa ggggggaatt gtatggagaa aaaaatttta
     2221 acagtttgtc catactgtgg agctggatgc caattatatc tagtagtaaa agataataag
     2281 atattaagag ctgaaccggc taatggtaga acaaatcaag aaagtttgtg tttaaaaggt
     2341 cgttatggat gggattttct aaatgaccct aaaattctaa cacctaggct aaaaaagcca
     2401 atgataagaa aacaaggtgt actagaagag gttgaatggg atgaggctat aaactataca
     2461 gcttcaagac taaatgaaat taaagaaaaa tatggacctg attctattat gggaacaggt
     2521 tctgcaagag gacctggtaa tgaagcaaac tatataatgc aaaaatttat gagagctaca
     2581 ataggaacta ataatgttga ccattgtgct cgtgtctgac atgcaccatc tgtagccggt
     2641 ctggcttact cattaggtag tggtgcaatg tctaattcta taccagagat agaaaatgca
     2701 gatgttttat ttatatttgg atataatgga gcagattcac atccaatagt ggcaaataga
     2761 atagtaaaag ctaaaaaaaa tggtgctaaa ttaattgtta ctgaccctag agtaactgaa
     2821 tctgcaagaa ttgctgatat acatctacct ataaaaggtg gaacaaacat ggttttagta
     2881 aatgcttttg gaaatgtatt aatagaagaa ggtctttaca ataaagaatt tgttcaaaat
     2941 catacacaag gatttgatga gtataaagaa attgttaaac catatacagc taaatatgct
     3001 gaaaagatta caggaatccc agaagagctt ataagaaaag ctatgagaga atatgcaaaa
     3061 ggtaaaaaag ctatgatact ttatggaatg ggtgtctgtc aatttggtca agctgttgat
     3121 gtagttaagg gacttgcatc aattgctcta cttacaggta attttggtag agaaagcgtt
     3181 ggaataggac ctgtacgtgg acaaaataat gttcaaggag cttgtgatat gggagctctt
     3241 cctaatgtat atcctggcta tcaaaatgta actgatgata agataagaga aaagtttgaa
     3301 aaagcttggg gggtaaaatt atctcctaat aatggttata gtttaactca agttccaaat
     3361 ttagtactaa aagagaaaaa gcttaaagct tattatatat ttggagaaga cccagtgcaa
     3421 agtgacccag atgcatcaga agttagagaa gcattagatg aattggaatt tgtcattgtt
     3481 caagatatat ttatgaataa gacagcactt catgctgatg ttatacttcc agctacttct
     3541 tggggagaac atgagggagt atatacatgt gcagatagag gattccaatt gatgagaaaa
     3601 gctatagagc cacaaggaga tgtaaaacca gactggcaaa ttataagcga aatttctaca
     3661 gcaatgggat atcctatgaa ttataaaaat actaaggaaa tatgggacga acttagacaa
     3721 ttatgtccaa gtttcttagg tgctacttat gaaaagatag aaactcaagg atgcgttcaa
     3781 tggccttgta agagtgaaag tatggaagat aaaggaacta tgtatctgta tgaaggtcaa
     3841 aagttttcta ctcctaatgg taaaggtaat ttgttcgctg ctgaatggag accccctatg
     3901 gaagttgaag atgatgaata cccatttagt ttatgtactg taagagaagt tggacactat
     3961 tcagtaagaa caatgactgg taattgtaga acacttagtt cattagaaga tgaaccagga
     4021 cgtgttcaaa taaattctaa tgatgctgaa aaattaggaa ttgaagatga tgaattagtt
     4081 agaattagtt caagaagagg aagtgtaata acaagagcaa cagttactga tagagttaag
     4141 gaaggtgcaa catatatgac ttatcagtgg tgggttggag cttgcaatga acttacaata
     4201 gcaaatttag accctatatc taaaacacca gagtataaat attgtgctgt taaacttgaa
     4261 aaacttgaag accaagaatt agcagaaaaa tgtgtaagag aagagtatca aagtttgaaa
     4321 gacaaaatga cagctacaaa tatctaatga tttattagat ttaagattta taaaaggcag
     4381 aaaggctaat attttatgaa caatatatac tcaaaaacta aattacaaga aaatagtaag
     4441 gataaagttt ttgaaaatag ttatagtcta gtggactaca aatatttaaa ttcttttaat
     4501 gtagaagtaa gcaaaataaa tgaacatgaa gtatcaatag ataatgaaga aataattgca
     4561 gaatatccac ttagttttat tttaaatgat aaatatgtaa atacattttt atgtacccca
     4621 tataatttag aggaacttat agtaggattc ttgaaaacaa aaggatacat agaaaacaaa
     4681 aaaagcttat taaaatttga gataaatgag agtgaacgtt ttgcaaaagt aactattcaa
     4741 gagaaacaaa aaaatcaaga tgagcaaatt atatttttaa attcactaga ctatataaca
     4801 tgtgctcctg taaagagcaa tataaaaata aattatgata cgatttacag tataatggat
     4861 aaaaatttaa attcatcaga attatttaaa aatactggtg gggtacatag cgtatctgta
     4921 tttaattttg ataaaaaaat agtcacttgt gaagatgtag caaggcataa tgcaatggat
     4981 aaggctattg gattttgtgt tttaaatgac ataacattaa aagataaaat tgtggctgta
     5041 agtggaagaa tatcattgga aatgatatta aaagcagcta tgactcaaat tcctattgta
     5101 gtatcaaaat cagcaccgac taatttatca atagagcttt ctaaaaaatt aaatataacg
     5161 cttattggat ttgtaagagg aagacggatg aatatttatg caaatccaca gagagttatc
     5221 aaaaagtgag aatgaatata tgtgtactaa aaaagaagag caaagatatt caaagtgtga
     5281 aatctgtgga gtattaattg atgatactga taaggtgtat aataacacat acaatttgtg
     5341 caaaaaatgt aaacgaaata taagtactga gaaaatagtg aaatatataa atatatatga
     5401 tgaaagtaag tagacatatg atgaaaataa ctagagggtt ggaaatatac aaattgaaag
     5461 gtgggataat atgaaaaata aaataggctc ttttgtaata gcagacccaa acaaatgtac
     5521 aggttgtaga gcttgtgaag tagcttgttt tacaatgcat aatcagcata ataatgtggg
     5581 ttatacagtt ggaacagttg atatacctgt tatacctaga ctttacttag taaaaggtga
     5641 caatttctgt atgccaatac agtgtagaca ttgtgaagat gctccttgtt taaactcatg
     5701 tccacaaaaa gctatagtta aagaaaacaa tattatgtct gtaaatgaag aaaagtgtat
     5761 aggatgtaaa acttgtctgc tagcatgtcc ttttggagct atagacttac ttccacaata
     5821 tgaagatggt aaagaagtag aacaagtaat tttagatgaa aataagaaga tagcttataa
     5881 atgtgactta tgtaaagaca atgaaaaaat agcttgtata agtgcatgtc ctcaggaagc
     5941 attaaaactt gttactccaa tagatgacaa aaaagctaag aatagacaag ctgcacttag
     6001 tttattgtta acaaataaat aaaggataca ggggtaggtg acgtttatga taataaatat
     6061 agataaggat ttatgtactg gatgtagaga atgttcaaag gtctgtccag taaatgctat
     6121 tgaaggtgaa gagggaaagc cacaagagat aaatttagat agatgtgtta tgtgcggtca
     6181 atgtgttcaa acttgtaaat cttatgcctc agttatagat gaaggatttg agtttttaca
     6241 agagaaaaaa caagaaagag agataccaga gtctataaat gaacctatat ttgcagcata
     6301 caatgtatgt gatatagata aggtgaagaa agctttaagt gacccaaatg tattcactat
     6361 ggtccaatgt gcaccagcag ttagagttgc tttaggagaa gattttggat attatttagg
     6421 atacttaggt gctgggaaaa tggcagcagc acttagagct ttaaattttg atagagtcta
     6481 cgatactaat tttgcagcag atttaactat aatggaagaa ggtagtgaat taattaaaag
     6541 agtgacagaa ggtggaactc ttccaatgtt tacatcttgt tgtccagctt gggtaaaatt
     6601 catggaaaaa aattatccta aattaactaa tcatttatct tcttgtaaat cacctcagca
     6661 gatgggtggt gcaatattta aaacttatgg tgcagagata aataatgtag atgcatcaaa
     6721 gatatttagt gtttctataa tgccatgtac atgtaaaaaa tatgaatgtg atagagaaga
     6781 aatggattct agtggatata gagatgtaga tgtagtatta actactagag aactagctta
     6841 tttaataaaa gacatgggaa ttgattttaa aaatttaaga gaagaaaaat ttgatagtcc
     6901 tttaggtagt tatagtggag ctggaactat ctttagtgtt actggtgggg ttatggaagc
     6961 tgctataaga acaggatatg agcttataac tggagaaagt attcctgatg tagaagtaaa
     7021 acaagtaaga ggagaaaatg gttttagaaa gtcaactatt aaaataggag acttagactt
     7081 aagagttgga gtagtaagtg gattaaaaaa tgtaatacca gttcttgaag acttggaaaa
     7141 aggaaaatta gatgtagatt ttattgaagt tatgacttgt cctgttgggt gtgtaagtgg
     7201 tggaggtcaa ccaaaaattt tactagaaga atataaggaa ttggcttatg aaaatagaac
     7261 aaaagcaaca tacgtgcatg atgaacattt agcaattaga aaatctcatg agaatcctga
     7321 tattaaaaaa ctgtatgatg aatatctggt agaaccatta ggagaaaaat ctcatcatct
     7381 attacacact acatattgta taggaaagga agtggctaag tgatgaacta ttttgttata
     7441 gcagaccctg ataaatgcat aggatgtaga acttgtatga taggatgtgt agttgcacat
     7501 tcagatgaag atatttttta ccaaaatcca gatgaaataa attttaatcc aaaactttct
     7561 gtcataaaga caaaagaagt aagtgctcca atacagtgta gacattgtga agatgctcct
     7621 tgtgcaaagg catgtccaaa tggtggtata gttagggttg gtaacacaat aaaaataaat
     7681 gaagaaaatt gtataggatg taagacttgt atgttagcat gtcctatagg tgcaatagac
     7741 atagttacat taaaagatgt agatgaagga aagctttgct ttagagaaag aatgacagca
     7801 aataagtgtg atttttgtat agaatcgcca gaagggcctg catgtgtaaa tgtttgtcca
     7861 actaaggcat ttactatagt taaggatgag gatatagata gtaaagttaa aaataagaga
     7921 aaattagcta tattgtaata taaaaaagat ggagttttat ttatgattaa aagctccatc
     7981 ttttttatta taatatttat atatctaaaa atcaattatt gcattagttt atctaatctt
     8041 tctttattca attttttcat tcctatagaa gttataataa gtaaagtata ggcaattata
     8101 actgataaaa ttataataga ccaagaaaat ctgtatccaa aattatcaac taacaatcca
     8161 aataatgttg aacctaatgc aaatcccact gcaaaaatta attgtacgat tccatatatg
     8221 ctaccaaact ccttttgacc aaagaaagag ccagttaaat aagcaggacc aatcatataa
     8281 gcaaatacag aaagaccttt caaaatggca aatacaaatg caaaagtaga agatttatct
     8341 accaatagta atgataaagt tgctataaga accaatacaa aagatataat aagtggcttt
     8401 gtttgaccga atttatcaaa caataatcct ccaaataaat taccaatgag agcaaataag
     8461 gcaaatgtgg agcctactgt tcctattaga ataggattta gataatttct aagataagtt
     8521 ggatattgta cagataatga tgaaacatat ataccaacaa atataaatcc aatagtcata
     8581 atccaaaaat atttaatttt tttagcttct tttaatgtat aaccccatac tgaaggagaa
     8641 attgtatcag atgctttctc ttttttacct ttagatttta ccttttcatt aatatcttta
     8701 ggcatcctga gtatgaataa tattacaggt ataccaacta ttaatgctat aattccaaat
     8761 attagataag aacgactgta tccttgagtt gctaataagc gaactgcaag tggttgtaga
     8821 aatatactac caatagaacc accagcaaaa gcaaagccca aagcttttcc tttgctttct
     8881 tcatcaaacc aagcacttat taaaacaggt attcctatag cagatataat tgtagcgcca
     8941 atttgaacaa tacatgatac agtgtaaaat tgccatagtt cagtacatat agaaaaagta
     9001 gcaaaagcaa tactagaaat tatacaacct aataaatata gtatcttaat attgacttta
     9061 gaaaataata cacctataaa aggtgatgca attgctgagg caatagtacc aatagtgaat
     9121 attagagaaa aagaagctaa actaaagcct tctccttcta caacataact tataaattga
     9181 ggctgtatac ttgctacaat actaaatgga acagcttgta ttaacataca accaagtact
     9241 ataacccaag catttgaaaa gtttttaatg ctaaatttat tgtttagtaa tgtattcatg
     9301 gtatctctcc tttaggttat gatttgtaga tattaatcta attattctaa ttttatatat
     9361 tagttttgga tagcctaact atttaaatat aatttgggta ttaatataaa aattaactat
     9421 aaaaattgat tattttaaat gtatggggta aattaaagta tataaataaa tgaaaggatg
     9481 gataaaatgt catttgaaaa aataaataat aataacctag ataaaataca caataggaag
     9541 tgtattatgc ttgtaaactt taataaaaaa gaattatcat taattaaaaa tgtaagtaac
     9601 tttgctggaa taagagacca tatcatctta agtgataaaa atggagagaa tataataaga
     9661 gacattttag atggaaatat atcaaatgaa tgtgaaaatg ggattaagag taagtctgta
     9721 atatttaata acactccaag tacacaagta aatgcatttt tagaagggct aaaaaaagtg
     9781 agaataaata gaccttttac agctatggta acagagacat ctattgattg gacattgaat
     9841 aatttaatag aaaatttagt tcaagaacgt aatgctatgt ctttaggaaa aacaattgaa
     9901 cataagtaaa tttaagatat cttatagtaa ttttaactaa tgtacattga gtatcatatt
     9961 atattttaga atagaaaatt ttaaatgtgg gtagatggag gagatttgag atgaaagttt
    10021 tatttacaat aaattatgga aaagaaaaat ttgataaaat aagagaatta ggctatgatg
    10081 tcatacatta ctctgaagat agtattgaaa ataatgagga tgtaaataca gcagatgtac
    10141 tagttacata caatccattt gagagattag atatttctaa gatgaaaaat ttacaatata
    10201 tacagacaac tagtgtaggt atagaccaac ttccaaaaga agaggtattg aagagaaata
    10261 ttaagatagc taataataga ggcggatata gtgtacctat tggtgaaagt attgtattgt
    10321 atattttaga aatatataaa aatagtgcaa aattttttaa acaacaacaa aataaagaat
    10381 ggaagatgga tttttctgta tcagaattgt atggacaaag agttggattt ttaggaacag
    10441 gtactatatc ttcagaagca gcaaaaagat taaaagcttt tggtgtagaa atatggggtg
    10501 tcaatactga tggaagtagt aaaaaatact ttgataagtg ttttgctact gaggatatga
    10561 atattgtatt tgaagaatgt gatatagtag ttgtagcaat gccttctaca aaaagtacag
    10621 atggaatagt aaacaaagat atgtttaaac ttatgaaaga tggttctgta tttataaatg
    10681 taggtagagg aaatacagtt aatcagaaag atttagaaga atgtgtagag aaatttagag
    10741 gggtagcatt agacgtattt gaaagtgaac ctttaaacaa ggacaataag ttgtgggagt
    10801 gcgaaaatgt catagtaact cctcataatt cttgggtgtc agataaaaac aaagagagaa
    10861 catttaatat ggtttataat aatttgaaac actatataat ggagaataaa cctgtagata
    10921 atgttgtaga catatcaaga ggctattaaa aataaaaaat tttgataaaa ataaaaactc
    10981 ttattagtcg tattaaagat actaataaga gttttttttg taataaattt aaagcccaaa
    11041 aagtgatata ctaaaaaata tataaatagg agataattaa ttatagggaa atttcaaaat
    11101 aatttaattt taaaaatatg aagaggtgca aaaagtgagc gtaaaagatg aaattaataa
    11161 acttatagag acaataaact accataatga aaggtattat aatcaagata gcccagaaat
    11221 atcagattat gaatacgata agttaatgaa ggagttaatt tctttagaag aagaacatcc
    11281 agaactaaag agaattgatt ctccaacaaa tagggtagga ggtaagccat tagataagtt
    11341 caatcaaatt gtacataaaa ctcctatgtt gagtttatca aatgcttttt cagagcaaga
    11401 tttgagagat tttgataaga gagttcaaga atatgtggga gaaaatgttg aatatgttgt
    11461 tgaatttaaa atagatggtc tttctgtagg tctaacttat aacaatggag agtttgaaaa
    11521 aggagcaact agaggagatg gagttgtagg agaagatatt tctcaaaact taatgacagt
    11581 aaaaagcata cctcttaaga taaatgataa aaaagaacta atagttagag gagaagtata
    11641 tatatcaaaa gataatttta gaaaagtaaa tgaacaacaa gaagaacaag aacagccatt
    11701 atttgctaat ccaaggaatc ttgcagctgg ttcattgaga caattagacc caaaactaac
    11761 agcaaagaga ccattagata tatttgtatt taatatggaa aatatagaag aatacgattt
    11821 agaaagtcac agtgaatcac tagagtactt agaaaaacta ggattttcag taagtcagag
    11881 ttataaagtt tgtaaaagta tagatgaagt tattgagcat attgaatact ggacagataa
    11941 caggggtaat ttagactacg aaatagatgg tatggtaata aaagtaaaca atataaaaca
    12001 aagagacgaa atgggatata cagccaaaag tcctagatgg gcaattgcat ataaattccc
    12061 agcagaaaag aagaaaacta aaatagtaga tataatagta gaagttggga gaactggtac
    12121 aataacacct tcagcaattc ttgaaccagt cagacttgca ggtaccactg taagtagagc
    12181 tacacttcac aatgaagatt atataagaga aaaagatata aaaataaatg atacagtatt
    12241 agttcaaaaa gcaggggaca taatacctca agttttagaa gttataaaag aagaacgtac
    12301 tggagatgaa atagatttta aaatgccaga aaagtgtcca gtttgttcag agccaacaat
    12361 aagattagaa ggagaagctg ctgtaaaatg tatcaatatg tcttgcccag cccaaataag
    12421 aaggggaatt atacactttg tgtcaaggga tgcaatgaat atagatggat tgggagaatc
    12481 tataattaca ctgctattaa atgaaaaaat tattcaagat gtatctgatt tatattatat
    12541 aaaaaaggaa gatgtagtcg acctagagag aatgggcgaa aaatctgctg acaatttaat
    12601 aaattcaatt gaaaaatcaa aagaaaatga tttatggaga cttataaatg gattaggtat
    12661 taaatttata ggagttaaag cagctaagat acttgcatat aactttaagg atttagatga
    12721 agtaatttct gcaactagtg agcaactaga agaattagag gaatttggaa gtataatggc
    12781 aaacagcgtt gtcgagtttt ttaaggaaga caaaaatatg aacgttataa acaagttaaa
    12841 aaatgtagga gtaaatacaa aatcattgga taatacaggt gaaaaagtag aaaaaatatt
    12901 tgaaggtatg aaaatcgtat tgactggtac acttcctaca ttaaaaagaa atgatgcaaa
    12961 agatatgata gaaaaaagag gtggaaaagc tacatctagt gttagtaaat cgacaacatt
    13021 tgttttggca ggtgaagaag ctggttctaa acttactaaa gcaaatgatt taggaattaa
    13081 agttatagat gaagagagat ttttagaatt attaaagtta tcatcgaaag atgaagtaaa
    13141 agctgtatta gagagttaaa aagtcgctta atttagtaga aaactactat aattaatgat
    13201 agaatagttc agtaagctaa taagattacg aaaaatgaca ataattaata tttgacaaaa
    13261 ttaaaaatta taaaaataaa tttataaaaa ataaaaaata tgagaaagga tataaaaaat
    13321 gaccagaagt tatgatagaa gtaatgaaca gataagacct gtaaagataa ctagagatta
    13381 tacaaagtat gcagaaggtt ctgttcttat agaaatggga gagacaaagg ttatatgcac
    13441 tgcatcagtg gaagaaaaag ttcctccttt tttaagaaat agtggtactg gatggataaa
    13501 tgctgaatat tctatgctac caagagcaac acaacaaaga aagataagag atgcatctag
    13561 gggaaaaatt gatggaagaa cacaagaaat acagaggtta ataggaagaa caataaggtc
    13621 tgttgtagac ttgaataaat taggagaaag aactatttgg gtagattgtg atgtgattca
    13681 ggcagatggt ggaactagaa ctgcatctat aacaggggca tttgtagcag ttgcagaagc
    13741 aatatataaa ttatataaag caaagacaat aaaagagatg ccaataacaa attttgtgtc
    13801 tgcaattagt gttggtattg taaatggaat acacatgcta gatttatgtt atgaagaaga
    13861 ttctaaagca caggtagata tgaatataat aatgactgat aagtgtgagt ttgtagaagt
    13921 acaggggaca ggagaagaga aacctttttc tagagcagat ttaaatttat tgttagagtt
    13981 aggtgaaaaa gggaataaag aattaataag gcttcaaaga gaagctttag gtgaaatatc
    14041 agatgagata ttgggtatgg attatggtaa tgaagtagtg attgctacta ataatgctca
    14101 taagctagaa gaaataggtg aaatcttaaa agattttgaa tataaagtgt actctttaaa
    14161 agatgttgat ttagcaggta tagagatagt agaagatgga aaaacatttg agcataatgc
    14221 gttgataaaa gctagaacaa tagcaaaaaa gactaaactt atagcaatat cagatgattc
    14281 tggcttagaa gtagatgctt taggcaaaaa acctggtgtt tattcagcta gatatgcagg
    14341 agaacatgca acagatgaag aaaatagaaa aaaactgtta aaagctatgc aaaatgtacc
    14401 aatgagcaaa agaaatgcaa gattcgtctc tgcaatagca gtagtttttc ctgatggaaa
    14461 agaatttgta gtaagaggaa tttgtgaagg aatgatagga tttgaagaaa aaggtaaaaa
    14521 tggttttgga tatgatagct tatttatagt aaaaggatat gataagactt ttggtgaaat
    14581 acctagtgtt ataaaaaatt caatcagtca tagagcaaac gcactaaaac ttatgaaaca
    14641 agagtttata aaaagggtag tgaagtaatg aaaataggca tagtaagcga cacacatatg
    14701 atgattaaaa atatggaaaa aacaatacca tatttgaaag aatgtgacct aataattcat
    14761 gcaggagata actttactga ttcaagatat attcatagca tgactaatgt aggtattatt
    14821 gcagttaaag gtaattgtga ctttgatgct gttgaggaag aagttgtctt tgaagtggca
    14881 aataaaacca tattcttatg ccatggagat aagtatggtg taaaatatgg aactaatatg
    14941 ttagaaaaaa aggcaactga agttgatgct gatatagtta tatttggcca tacccatacc
    15001 ccatttagag aaattaaaga tggtgtactt tacataaatc ctggaagcac ttcacttcca
    15061 agaggagtat cgtataaaag ttttgtcatt atggatatag aagaagatga cataaaaatt
    15121 gaagaaatac gcatataaag gtgtatttat ctaaaaaaat ggcaaaaaac gctcaaaaaa
    15181 ttaaatttta tcaaataagt acaacgatat taatggaaca cttgtaaata aaaaaactta
    15241 tgtgatataa ttaagtggtt gaattataaa tagaatgaat tataataccg ttatcgggta
    15301 ttacataaat atcactagga ggattagaag gatgaaagca gaactaatta aaaaagaggg
    15361 taataaagtt actttaaaaa taacagtaga taacaataaa tttgaagcag cagtaaataa
    15421 ggcttataat aaaagcagaa ataaatacaa tatacctgga tttagaaaag gtaaagctcc
    15481 aagaattgtt atagagactc aatacggaaa aggtatattc tacaatgatg ctatagaaat
    15541 attattccca gaagtttatc cagaagctat caaggaatta gacatagacc caatagacaa
    15601 tccagattta gatatagaag aaataagtaa agacaatggg ttggttatgg ttcttaatgt
    15661 agaagttaaa ccagagtttg aattaggaaa ctacaagggt atagaaatag ctaaggttga
    15721 gaatactgta agtgacgaaa atgttgatgc taaactacaa gaaatggttg aaaaaaatgc
    15781 cagattagta agtgtagaag acaaagcttt agaagatgga gatacagcta taatagactt
    15841 cgaaggtttt gaaaatggtg tagcttttga tggtggtaaa ggtgaaaatt ataacttagt
    15901 tataggttca aacacattta taccaggatt tgaagaacaa ttagtaggta aaaaagctgg
    15961 tgaagaagta gaagttaatg ttactttccc agaagaatat cactcacaag atttagctgg
    16021 aaaaccagtt gtatttaatg taaaaataaa tgatgtaaaa gtaaaagaat tatctgcact
    16081 tgatgatgaa tttgctaaag atactagtga atttgattct ttagatgaat taaaagcaga
    16141 tgtaagagct aagttagaag aagaagctaa aaatagagca gatgcagaaa ctagaaattc
    16201 agtagtagaa aaagttgctg aaaatacaga aatagaaata ccagaagtta tgatacaaca
    16261 tcaaatagat aatatgttaa atgaacttaa ctaccaatta caatatcaag gatttggatt
    16321 acaacaatta ctagagatga ctggtagaac tatggaagaa ttaagagaag agagaaaaga
    16381 agatgctaaa aaattagtta agtcttcttt agtattagaa gcaataacta aagctgaagg
    16441 aattgaagca acagaagaag aatttaaggc tgagttagaa aaaatggctt ctgcttacaa
    16501 tatggaagta gaaaaaatag aagcttcttt aagagatgct gacaaagaag atataaaagg
    16561 tcaaataaaa ataagaaaaa caatagattt attagttgat aatgcaacaa tagcttaata
    16621 aaataaacgc taactaatat agttgacgtt atgaaatgtt tagtatataa tgatgtgcaa
    16681 atataattta gttaaatgcg tataataatt tacagtagct taaaaaaagc tactgtaaat
    16741 tatatagaat aaagggggta tgaaaccatg gcattagtac ctgtagttgt tgaacaaaca
    16801 ggaagaggag aaagatctta tgatattttt tctagactat taaaagacag aataatcttc
    16861 ttaggagatc aagttaacga tgccacagca gggcttatag tagcacagct attattttta
    16921 gaagctgaag acccagacaa agatatacat ttatatataa attctccagg aggaagtata
    16981 acttctggaa tggctatata tgatactatg caatacatca aaccagatgt aagtactata
    17041 tgtataggta tggctgcttc tatgggagca ttcttgttgg cagcaggagc aaaaggaaaa
    17101 agactagcac taccaaatag tgaaataatg atacatcaac cattaggtgg agctcaaggt
    17161 caagcaacag atatagaaat tcatgcaaag agaattttaa aaataaaaga aactttaaat
    17221 gagatattat ctgaaagaac tggtcagcca ttagaaaaaa ttaaaatgga tactgaacgt
    17281 gataacttca tgagtgcact agaagccaaa gaatatggtt taatagatga agtatttaca
    17341 aagagaccgt agatagagag aggtgttaat atgtctaaat atgaagaaaa acgacaatta
    17401 aagtgttcat tctgcggtaa aaatcaagat caagtaagaa gattaattgc aggtccaaat
    17461 gtatatattt gtgacgagtg tgttgaactt tgtgacgaaa ttattcagga agaaatagaa
    17521 gatactatag atgaagatac tacatcttta ccaaaaccaa aggaaatgat ggagattcta
    17581 aatgattatg taataggtca agaaaaagct aaaaaagctt tatcagttgc ggtttataat
    17641 cattataaga gaatatacag taaaaaatct agtagtaagg atatagaaat tcaaaagagt
    17701 aatatacttt tattaggacc tacaggttct ggaaagacat tacttgctca aactctagca
    17761 agaactctaa atgtaccttt tgcaatggca gatgctacat cattgactga agctggatat
    17821 gttggagaag acgttgaaaa tatactttta aaacttattc aagctgctga ttttgatata
    17881 gaaaaagctg aaagaggtat aatatatata gatgagatag ataagattgc aagaaaatct
    17941 gaaaacccat ctataactag agatgttagt ggtgaaggtg ttcaacaagc acttctaaaa
    18001 atattagaag gtactgttgc aaatgttcca ccacaaggag gaagaaaaca tcctcatcaa
    18061 gaatttttaa agatagatac tactaatgta ttatttatat taggtggagc ttttgatgga
    18121 ttagagaaga taattcaaaa acgtggtgga gataaaacac taggttttgg agctaaaata
    18181 gaaagtaaaa aagaattaga tttagggaag ctgtatgaaa aagtacttcc agaggattta
    18241 ttaaaatatg gtataatacc agagtttata ggaagaatac cagttttagc tactttagag
    18301 ttgttggatg aagatgcatt aatgcagata ttacaggaac ctaaaaatgc tctagtaaaa
    18361 cagtataaaa agttattgga gttagatgat gttgagctag aatttgaaga aggtgctctt
    18421 cgtgcaatcg ctaaaaaggc catagaaaga aatacaggag ctagaggact tagaagtata
    18481 gttgaaagcg taatgatgga aactatgttt gaagtaccat ctagagataa tataaagaag
    18541 gttatagtaa cagagaaatc tgtgaacgaa gactcagtaa atcctatcat agtacttaag
    18601 gatcaagagg aaagtgcata agatgatgaa aaggagcttt aaaagctcct ttttttatgt
    18661 taatatgcta aatataaaat aggtatcatt attataaaaa tgtgagtaat atacctagaa
    18721 taccaaagac gaatacaatt gcaatggaac caatttttcc tttatataaa agataaaatg
    18781 atgctataaa tgcaattatt ccatataaat cccaattaac ttctttggca gatgaaaaag
    18841 ctgctgataa tatgaatcct agagtaattg gtctaagaac tgtcaataag ttttctacct
    18901 ttttattatt tttaaattta gaaaataact ttgatataat agttagacct attaatgcaa
    18961 aaattgtaac accaatagtt gcagctagag aaccaaagaa gccagcagtc ttaaatccta
    19021 caaatgtagc actatttatt gctacaggac ctggtgtaga ttgtgatata gcaagtaaat
    19081 ccaaaaactc ttgattagtt ataaagtgat atttagatat aaattcttgc tggataaatg
    19141 gtagcatggc ataaccgcca ccaaaactaa atgcccctat tttaaggaat attaagaaga
    19201 gtagtattat ttctgacata gactatatac acctcctcca attattaaat aaatagggct
    19261 tatattgaat acaccaacag ataaaactgc tataattaat agtagtatgt tagttttaga
    19321 ctttggtagt ttcttgaaca tgctcgtaaa agaatacaat ataagagcta ttacaacagg
    19381 cgcaactcct ttaaaaaatc catctaaaat tttagatgat gaatttttaa agtatagata
    19441 cgataagact aagactatac aaaatgatgg taaagcagag cctaaggtac atataagaac
    19501 tcctggtata ccataaagtt tatatcctaa taatacagat atattaactg ccagtactcc
    19561 aggataactt tgagctatgg ataaataatc aataaattct tcctgctcta aaagtttttg
    19621 tttgtttaca agttcatctt gtataagagg aaccatagcg tatccaccac caaatgtaaa
    19681 cgcaccaatt tttaaaaagg ttaaaaacaa tttcaacata atagaaacct ccaaaagata
    19741 aataacatct aagtatacta atattaatat tatatcataa taaaaagata atatagtatg
    19801 atagtattct aagaatttat cattaatgtt gaaaattaac ttgttcaaat atataatatt
    19861 aaaagcaaag caattagact agaaaaggag taatgttgat ggaacaaaat tatactaaaa
    19921 tagatcatga attaccatta attcctctaa gaggattagc tatatttcca tatatgatat
    19981 taaattttga tataggtaga gagatatctc ttaaagcttt agaccaagct atgatggatg
    20041 aagaacttat atttttaact tctcagaaag aagcagaggt agatgagcca ggagaagagg
    20101 atttttatca tgtgggaaca atctgtaaag ttaagcaaat gataaaatta ccaggagata
    20161 cagttcgtgt tttggttgaa ggtgtatcaa ggggtagagt aaagaaaata gaacaagaag
    20221 atgggtattt cagagcagta atagaagaaa tagtatttga tagtgataat ttggatagtg
    20281 aaacagaagt tgaaattgaa gcatttgtaa gaaatgtatt tgatgccttt gaagagtata
    20341 taaatattgg aaatagggta tcaccagaaa tattaatatc tttagctgat atagaagatg
    20401 tagatagatt tatagatacg atagctgcaa acatatatct aaaatcaagc caaaaacaag
    20461 aaatattaga agaatttgat attagaaaaa gacttgaact tatttattct atattattag
    20521 aggaaataga tatattaaaa atagaaaaga aaataacttt aagagtaaaa aaacagatga
    20581 ataaggttca aaaagaatat tatcttagag aacaattaaa agctatacag aaagaattag
    20641 gtgaagaaga agatataaac tctgaagcag atgagtatag agaaaagctt aaaaaaataa
    20701 aagcacctaa gacaactaaa gagaaaatag aaaaagagat agataaattt tcaaaaatat
    20761 cttctatgtc tccagatgta tctgtaagta gaaactattt agatactata ttctcattgc
    20821 catggaataa agagacaaaa gataaattag atataactaa ggcaaaagat atattagatg
    20881 aagaccatta tggattagaa aaagttaagg agagaatact agagtatcta gctataagaa
    20941 ctcttgctaa gtcacttaaa gggcctataa tatgtctagt tggtcctcca ggaacaggaa
    21001 agacatctat agtaaaatca atcgccagag cattaaatag aaaatttgta agaatatctt
    21061 tgggtggagt tagagatgaa gcagaaataa gaggtcatag aagaacttat gtaggttcaa
    21121 ttccaggaag aataattaat ggggtaaaag aagcccaaac aaaaaatcct gtattcttat
    21181 ttgatgagat agataagatg gcagcagatt ataaaggtga cccagcttca gcaatgctag
    21241 aagtattaga cccagagcaa aataaggatt ttgtggacca ttacttagaa ataccttttg
    21301 atttgtcaaa aatactattt gttacaacag caaatagtct aagtaatata ccaagaccat
    21361 tattagatag aatggaagta atagaagtat ctggatatat agaagaagaa aaattaaata
    21421 tagcaaaaaa atacctactt ccaaaacaaa ttaaagaaca tgctttaaaa gaaaacttta
    21481 taaaaataga tgatgaaact ttaagaagta taatcaacca ttatacaaga gaagctggtg
    21541 taagaacact agaaagaact attggtaaga tttgtagaaa ggttgccaaa aaatacgttg
    21601 aagacccaac tcttgaagag gttgttataa ataagtctga cctagagaca tatcttggaa
    21661 aagacatgtt taagtatcaa cttgcagaag ttaaccctca aataggatta gtaaatggtc
    21721 ttgcatggac agaagttggt ggagttacac ttgaagtaga agtaaacgta cttaaaggta
    21781 aaggagaaat tgtattaact ggaaaattag gagatgttat gaaggaatct gcgaagacag
    21841 gtatctctta tataaggtca atagtggaca agtttgatat agaccctgaa ttttataaga
    21901 cgaatgatat tcatatacac attccagaag gtgctgttcc aaaagatgga ccatcagctg
    21961 gtataactat ggctcttgca gtaatctcag cacttactaa aagacctgtg cctggaaata
    22021 tagcaatgac aggagagata actttaagag gtagagtctt agcagtagga ggagttaaag
    22081 aaaagttatt agcagctcat agagctggta taacaaaagt attaattcct aaagaatgtg
    22141 aagctgattt agatgaaata ccagaaaatg taaaagaaaa aatggagttt gtgcttgtag
    22201 aacatatgga tgaagtatta gaacaagctt tattaaagag tggtgaaaat aatgaaaatt
    22261 agaagttcag agataactat gagtgctgtg aataaatcac agtatccagc agaaggaatc
    22321 ccagagatag cattggctgg taggtcaaat gttggaaaat catcaataat aaatacattg
    22381 cttaacagaa gaaacttcgc tagaaccagt caaacaccag gaaaaactag aactataaat
    22441 ttttatctaa taaataatga attttatttt gtagaccttc caggctatgg atatgcaaag
    22501 atagctaagt ctgaaaaaga aaagtgggga ggtataatgg aacgatatct agaaagtaga
    22561 caagagttat gttctatatt cctacttgta gatattagac atgagcctac agcagatgat
    22621 aagcttatgt atgaatggat aaagcacttt ggatataact gtgtagttat tgcaactaag
    22681 gcagataaaa tatctagagg acaatatcaa aaacacataa gtataataag aaaaaaattg
    22741 caaatggaaa gtagtgaaaa agtaattcct gtttcttcac taaaaaagac tggtgttgaa
    22801 gaattatggg aagagattgt aaatcaatac aatcaacatg gatatgaaat aacagtagat
    22861 taaatttaaa atgttaaata ttgacatgag atttatttga ataataaatg taataatagt
    22921 tagttttaat tatattaata ttaaagcaaa atataggatg ccttaaaata gttttaatga
    22981 actttttttg gcatccttat tgtttataaa caataaatat cagtaatttt gttaaatact
    23041 aataaatttt agtaaggtat gggaaaatta gtataaatta gttcaactaa aaaattattg
    23101 acttatgtag tcagcaataa atagcattta agaaatagat ttatctgttt ttctcataaa
    23161 gaaaacagtt agtataagcc ctataaaaca tactgatgct gcaatccaat aagactgatt
    23221 gctagctata acataggctg atgtgtgact tttaaacttt tgattgtatg taagactttg
    23281 tgcaaaaaaa ctactaaaaa atgctgttcc aagagcaaat gataaattac gaatagttgc
    23341 aagaaaactg cctccgtatc cctgtttttg aggaggaaca tctgctaaaa tactactatt
    23401 gtttggtgaa ctaaatgctc ctagcccaag tccaaaaata actaaacata caattaaaag
    23461 atacaatgga gaatctgctt ttaaaagtgc cattccaact agtgagatgg tcataaatga
    23521 aaaagatgct gttagaatgt tttttgcacc gtgtttatca gaaagtgaac ctgcaatagg
    23581 tgctataagt accataataa gtggatgtac agtcattaca taaccagcca tcataggaga
    23641 taaatttttt aattgttcta aataaaatgg aagtagaaaa gatactgcca tttgaggaaa
    23701 ataacaagct actccaatga tattgcctac agtaaagttt ctatttttaa atagaggtag
    23761 gtcaataaag ctttgttcaa attttacttc tcttaatgcc aacaataaga agattataag
    23821 tgctgttata cctaaataca atcttgtatt acccttctca ttcattgcaa gtaatataag
    23881 cgtagtgcaa gatgcgaaaa gtagaagacc tggaatatca aagctcttgt tttgttcatc
    23941 caaaacaggt ttaggtagaa atttaatgcc aagaaagact gctataatac caataggtat
    24001 attgataata aaaatagaag gccatccaaa atgttctaaa attatacctc caattacaga
    24061 accactcata tttcctatac caactacagc tccagtgatt cctatagctt ttcctttttc
    24121 atttgctgga aatgcattgg aaactatacc aagcccagta gccattaaaa tacttgctcc
    24181 aactgcctga aagattcttg aagaaattaa tgttgataaa ttgtttgaca tactacacag
    24241 aaaagaacct attgtaaata taaaaaatcc taatgtgtat agacgatttg aacctacttt
    24301 atcgccaagt tttccgaaaa ataacatagt agctgttaca acaagcatat aagctgttac
    24361 aacccactga atttgtgaca tgttaatttt aaagtcagca gcaattgtag gatttgcgat
    24421 atttagtata cttccatcaa gagtagacat aaatacacca atacagatga taatcaatac
    24481 tatccatttt tgtttcatga taacactcca atcttaatag attttataag tacgatataa
    24541 ttgtaaaata aacaacaaaa aaagcacctg tatattaggt accttgaatg tgtctaattt
    24601 catgaataaa tacggtagtt gaaggatttg tatagattac tcatgtaaaa ttacataaag
    24661 gtactcatat actattttct tctaccatcc agattttact gtcggctttg gaatatgacc
    24721 aaattatgcc ataaggctcg cgggctttta ccgccgatag ggaatttcac ccgtttatga
    24781 agaaaaacag atgtattttg taatattatt ttaacataaa attataatgg aaacaattgg
    24841 atttaagtaa tatccagctg tattacaata caaatatttc aatatggaca cttaaattgt
    24901 atttatagat agaataagga acagtatgag aatctataat aattagataa aattaacctg
    24961 ttattagtat tagatggtaa gttttaaatt tatcaaaatc agaaagctgt aataaaattc
    25021 ttataatatg aatatcttaa ttaagagata ttgtaaatta tgaggaggcg ctaatatgga
    25081 aggaaaggta aagaaggcat tcccgggtgg aaatacatca caaggctcat attctttttt
    25141 tgactatata attcctgaaa atgtaaaaag agttttttgt ttaaagggtg gacctggagt
    25201 aggaaaatcc tcacttatga aaaaaattta taaagagttt ttgcatagag gatatgatat
    25261 tgatttatat cattgtcctt ctgacccaag ttcgttggat ggtttagtta taaagaaatt
    25321 aggcgtggta cttatggatg caactgcccc acatactatg gacccaaagc tacctggagc
    25381 tttggatgag ataattaatt taggtgatta ctggaataca gaagaattag aaaagaataa
    25441 acaagaaata tcagtttatg ataaagatat tagtaattca tttaaaagag catttaagtt
    25501 tttaaaatca gcagaaccaa tctttagaga catagaagaa aaatattctg attgtatgga
    25561 ttatggaaaa gtaaatttgg tcacagaaga atttataaaa aggttatttg atggagttaa
    25621 aagcacagga aatctaaaaa gagaaaagca tttatttggt tcagctataa ctcctgtagg
    25681 gtgtttagat tatacagaaa acattttaaa agatgtaaag aaagtctatt atttagatgg
    25741 agaaatagga actgggaaaa ctacattatt aaacaaagta tataagaaag ctattgaaaa
    25801 aggtttaaat gtagaagttt atcattatcc acttattcca gagaaaattg aaactgtact
    25861 attaactgat ttagatatag gcataactat aagtacgact tttaaagaag aagatacaat
    25921 tgatttaaat cagtttttaa atagagaaaa actacttaaa tatgaagaag atattaaatt
    25981 tgatgaaaaa gttttagatg atttaatttg ttgggcagta ctaaatctta agagagccaa
    26041 gtctaaacat gatattatag aaagttacta tagtcctaat atgagatttg atgaagtagg
    26101 gaaattaaga gataagttaa ttaaaagaat tttaaaatat gaggaaaact tataaacgca
    26161 tatgtttaaa attaacgtta gtaataatat aatttttagt aaattggtgt agctttgagg
    26221 ctatgccatt ttactttttt tgctaaaaaa tgatattaat aatctccaaa gccattattt
    26281 gtttttcata attattattt cctttcctaa tttatgtttt tttataatat cacaacaatg
    26341 tttattgaaa gtttatataa ttttaagaac atctaaaaat gcatacttga tattggtaaa
    26401 ttagtgatag gagttcaatg tatgatatta tatactaaag atgaatttta atatataact
    26461 ttgatataat aagcatatgt aaatgtttaa tatttaatta aagaaaatta tcatataaaa
    26521 ataaatttgt aaggagcaat acagtatgaa aaaacaatat gtgtttataa tactagcaag
    26581 tactatttta ggtatacttg ttggaaccat atttagagat tatagcaatg aagaaacagt
    26641 ttatacaagc aaagacaagt caataagaaa agaaataaaa atgacaagaa aatctataaa
    26701 agaattaacc aaagagaaag acaagttaga taaagaaatt aattcaatga aacaaaatca
    26761 aaaaaattct gaagaaatat cagaaataga aacacttaga gatagtctat catattcaga
    26821 tgtaaagggt agtgggataa tattaagtat agatgctata gatgaaaaaa cagggaatat
    26881 agctaatagt atagactata ataagattct tgtaaaagtt ataaatgaat taaaagtaaa
    26941 cggaggaaaa tacatagaaa taaatggtca aagaattaat caatattcag aagttgtact
    27001 tgcaggaagc catataaata taaatggagt tcctgtagca caaccatatg aaataaagtg
    27061 cattggagat aaggagaaac tatctaatta tgtagataaa ggtagtaaat atttagatag
    27121 catacaaacc aattatccaa tgaaaataag cataaaaact gaagaaaata tctctatgaa
    27181 aaaaatgaat atacctaata aattaaagta tataaagggt gattaagatt atattagagg
    27241 aaagcagttt gccaatgtta ataccagaaa aacttgcaaa aaaatataat gtatttccag
    27301 ttgagtttca agatgataat ttagttgtag aaatagaaga tgaggatatt tatgctctac
    27361 aggacttgag attagctaca ggtaaagaaa ttatattgaa gaaggagaaa aaagaactta
    27421 taagtgaaaa gattgaaaaa tattataata gctttgaact tgatgaggat tatgcaaaaa
    27481 aattatttga aaatctttta gaaaaagcta taaaagaaaa cgcaagtgat atacatatag
    27541 aaccatttaa agaacattta atagtgagaa tgagggtaga tggagaacta aaggaagtat
    27601 caaagttttt agttgatgtc tatccatcat tgtctacggt tataaaatta aaggcatcaa
    27661 tggatataac agaaaaaagg cttcctcagg atggtagggt ggatataaag ttaggtggaa
    27721 atttaattga cattagagtt tcatcaatac caactgtcta tggtgaaaaa atagttttga
    27781 ggatacttaa tagagatact tttttaaaag acaaattaga acttggtttt tcagaggaag
    27841 ctataaaatc tataaatcgt ataataaata aaagagcagg aatattatta gtgacaggtc
    27901 ctacaggtag tggaaagact acaacagtat attccttatt aaatgattta aaaggaataa
    27961 ataaaaatat tatgactata gaaaatcctg tagagtataa gatggatggc ataagtcaga
    28021 ttcaagtaaa ttcaaaagta gggttgagct ttgatgtagg tcttagagcg atattaagac
    28081 aagacccaga tataataatg gtcggagaaa taagagatgc agaaacagct aagatagcag
    28141 taagagcagc tataacaggt catttagtta taagtacttt acatactaat gatgcagtat
    28201 cttccatagc tagattacta gaaatgcaaa taccaccata tcttttaaat gcctctctta
    28261 taggtgttat atctcaaaag ttggttagaa aagtctgcaa tcattgtagc catgagatta
    28321 tgataaaaga taattttagt ggagatgtaa atactaaggt agctgtaggg tgtgaaaaat
    28381 gtaatgataa aggatacttt ggaagaacag caatttatga aatcttagaa attaatgatg
    28441 atataaagac atgtataaga aatatggagg attctagtac tattaaagat gtagcacaaa
    28501 ataatggaat gataacattt gaagatagtt gtaaaagact aataaatgaa aaaataacga
    28561 cattagaaga gtgtttagta gtaaatgaaa tgatttaact gaattgtata gttaaacttc
    28621 gtttggtcag ttgtctttat ttaatctatt ttaattaaga tgtttgagta taaattatat
    28681 ttaagtgaaa taaaattaaa tgcattaaat tagaaaatat taagtttatt taattgtaat
    28741 taatataatt tggaaggtat tatagtacat gagattatct aaaaatatat tttataattt
    28801 agaaaagttt tatttagtat taaatagaat cagtgataaa gaaattagag taatttgcaa
    28861 agagataggc atattgctgg aatctggatg tgaaattaca aaaatatttg aaatcattga
    28921 gagtcagtca agtaaaaaag taaagaactt gcttagtata gtatcaaatc atattcaaaa
    28981 aggtaactca atagcagaat catttcaaat aacaggtata ttttctaaat tctttataag
    29041 tatgataaaa gcaggagaaa ctagtggaaa cttagatata ataatgagtg atttatctaa
    29101 ttattatgat aaagaataca aattaaaaat gaaaataata actatttcaa tatatccaat
    29161 aatacttata atattatcta tcttatcaat gttgtttata ttcgtatttg taatacctaa
    29221 ttttcaggtg gtttttacca acaatggaat tgaaccacct ttaataacta gagttttaat
    29281 gggaatatct acagtggtta caaataactt agcttatata atatttagtt ttatattgtt
    29341 ttcaatagga agcatttatt tctttataac aaatgataat ataaaaaaat tgattaattc
    29401 gttaaaattc aaaatacctt ttataaaaaa aattaatcag ttagtagcta caacaagatt
    29461 ttcaagggca ttgtccgttt taattagcag tggaatacaa attgtggaag ctatagatat
    29521 gtcagctagt gttatagaca acgaatttat ttatgaaagg ttaaagattt ctaataatta
    29581 tataaaaaaa ggaaatagca taggtaaatc tttgaattta gcaaatattt ttccaaaatt
    29641 atttatttca atggcaaata caggagaaga aagtgggaaa ctagataaaa gtctaaatgt
    29701 aataaataaa ttttatgaaa atgaattaaa taacaaaata gagcaaataa tgaaatttat
    29761 agaacctatg ctaattgtat ttatgggatt aattattggt acttttattt tagcaatggt
    29821 tgttcctatg tttgatgcaa ttactaattt ttagagagaa aaataaattt tattagatga
    29881 aacttttgaa aaggagagaa tatttaattg ataaatttga taaataaaaa acgaaaagga
    29941 tttacacttg ttgaaatgat tgtagtagta actattttag gcgttatatc tagtatagca
    30001 ttagttaagt atagtaaggt tcaagaaagt gccaaattaa atgcagacta tacgaatgct
    30061 gctaatatag taaccgcagc tagcatggca attaatgatg atgaaaagac aatagactct
    30121 ctaagtgtag agacattgaa ggaaaaggga tacctaaata ctgttccagt tcctcagagt
    30181 acatcaggta aattcgaact tgtcataaat gatagcggaa cagatataag cgtaaatata
    30241 aattctaagc aattttatcc aaaataatat tttatattga ttgctgtaag ttttaaaatt
    30301 atttttattt aagaaggaga tggctataat taaaaaaatt ctaaacttag aaataaatga
    30361 tgattatata aggcttgtat ttttaagaaa aagtcataaa aaagttttta tagataaatc
    30421 aattataaaa gagattaact ttgatataaa aaatgatagt aattcgattg agaagattaa
    30481 atatatagaa gatgtagtta gatttgtaaa agaggagatt ggtaaatcaa aaatattata
    30541 taaaaattta tactttaaca ttcaaattca ggatatagtc attagaaatg ttgaagttct
    30601 aaatactaag aaaaaaagag acatattaag catgattgaa tttgaaataa cacaatacat
    30661 tccaataaac ataaataact attcaataaa atataaagtt ataaattcta aaaatgataa
    30721 attgaatata caagtaattc ttttcccaaa gaatattatt caattaatta agaacatttc
    30781 agaaaatcta aatatgaatc caaagactat aaatataaat tttgatatat tacaaaagct
    30841 tataaactta aatttaatag agaatttttc agaagaaggg gtatttatag aatgtaaaaa
    30901 aagtcagtta attataaata taacaaaaga taaaaaaata agagaaacat acttgctatc
    30961 aaaagaggga aaatcatatg agttcataaa aagattatta aggggtttta agtatgttta
    31021 ttactatggt atagaaaatt gttttattga agaatacttt aaatatacag ataaatctat
    31081 tgaagtaaat ccattaagat taaaagataa agtaaaagta gaaaataatt ttgatgcaga
    31141 tttagaagac aataggataa attatataaa tagtatagga atgattattt aagtgaaagt
    31201 ggataaaaca ataaattttc ttaatggatt taaaaaaact aaaccatgta ataaaatcta
    31261 taaaataata atagtcttaa ttttaacgtg tattttattt aaatcattta tgtatgtgaa
    31321 atctataaat gaacttgaag attatataag tcaaaataag ctagtggata aatcaaagaa
    31381 cactttacca acaacagata aaactagaga gaaattttct aaaataaaac ttaatgaaat
    31441 aaaaaaaata tatgaaacaa ttggaataaa tagtattact aaaatatcaa tagataaaaa
    31501 taagctagag atggaaggtg tgtgtagtga tttagatgtt ttaaacaatt taaggaaaat
    31561 aaagtacata aaatattatt acatagacag tattgtaaga gatggagaat attatagatt
    31621 tagaataaaa agttagatgg gtgattattt ttgagattaa ataatagaga aaaaaatcta
    31681 cttaaaatat tgttaatatt agttattata ttggcaagtg atatagcagt aagttatttg
    31741 gaagaaaacc taactagttt gaagaatgaa aagcaaaata taataaataa aataaataat
    31801 caagataatt atcaaataaa ggataataag catatagatg ataaatatat agaaagtgtt
    31861 gaattaaata aaaatgaaga attgaaaaat aattttaata actcattttt agataaaaaa
    31921 acaaataaag atatagtgtt attagtagaa aatgaactga gagatattgt gaatgtagaa
    31981 tatattaatc aagaaaatag tttagataat cacaataaaa aacaagttaa tttagaaata
    32041 aaagttaatg gagaacttgc aaatgtgttg aaacttgaag gtgtaatgaa gaagttaaga
    32101 ctaaataata aacttgaaaa gatagaaatt aacaaattaa aagtcaataa ttttgatgtt
    32161 caaaatattc caaatgaata taaacaagaa aaagacaata ttgttgaatg taaaatgaca
    32221 gttagagtca tataacaaaa aagaatagat aaagtaaaaa actattatca attaataaaa
    32281 ttttatagag gaattagttt tacgataatt catttctaca agagggattg ttgatgattt
    32341 tatcgaaatc ctaattaaag ttaatatatt gtaccaaaat ataagtgaat ttaatagaat
    32401 atttacttat actatagatt gattaataaa aaaatggtat aataacaaat tgtactttaa
    32461 tttaataata agggagtgga agtcatattg ttagggagaa atgagctatg cccttgtgga
    32521 agtggcaaaa aatacaagaa atgttgttta aataaagata ttgtttcaga aagagcatgc
    32581 agaaaaactg aactttcaca aaaacaatat acagatttat attcaaagtt atatgattat
    32641 tcaagacagg ataagtttaa agaagaatgt gaaaaagcta aagaaatgtt ttatataatg
    32701 caaaatgata gtgtaaatga aaaatttgaa agatttttta atacttattt aatacaagac
    32761 cacataatgg aaaataaaaa ggttatgaca gttggttttc ttgaagaaaa tggtgcaaac
    32821 ctaagtcaat cagaagtatc aatattaaaa agtatgtttg aatcatacgt aagtatttat
    32881 gaggtaaaag aagtttctgc tggaaaaata gtattaaagg actgtttaaa tggaaatgaa
    32941 ctttgtacag aagatgtaaa acttctgaga agttttaaaa tcggaagttg tatgatagct
    33001 agaatagttg atattgaagg aactaacata ttgatagata taactataag tatatcaaat
    33061 gaggttaaag atataatttt aaaggatatt atgaatctat tcaatcaata tcaagatgta
    33121 tacaaagata tgaagtcgtt cttaatttat catacacata tactatacaa gtatatacaa
    33181 cagcttttag acccaagtat agctgaatat ttaaagagag aaagagataa aaacgataca
    33241 aaagttaatt cagaaaatga attagctgaa gaagaatgta aagttttaga tactcttaaa
    33301 gaaaatgtag aaaaagaaaa tatagatgca tgtatagagt tttggaatga atataagaaa
    33361 aatcatgaaa atataaaagg agctgaaaat ggttgggcag ctgctgttga atactatgtt
    33421 aaaaaagaag caggacaacc tataactcaa gttcaaatat caaagaaata cgatataagc
    33481 tctagtacac taggaaagag atacaaggat ttaaaaatat cttaattaat tataaaaagc
    33541 tttaagtcat agataaagaa tctatagtat aatatatgta taatgtttag aaagaggtat
    33601 taataatgag tcttagttta ggaaatgcaa ttgctgtatc aacgaacttt ataaaatcta
    33661 acccagctgt acctgtgtac aacggagaag aaatatatgc agttatttat catatagaaa
    33721 taggatataa taatggtgta tcacctgata ccaaacaata tataataata gactataaag
    33781 agtttgattt atctatggat aaaaaaaatg aactaataaa tattgctaaa gagtactgta
    33841 aatctaaagg tattgtagaa aacgaaaacc ttgaaataaa ccttttagac aatgaagaag
    33901 cagagaactt cttagaaaag gtgtttaata atatgaaggt tataagaggt cttataacta
    33961 aaaaaggata aataaatgga ctatatagga atagaaaata taacacctta tgaaaataca
    34021 tatgaattta gtgtatatga atatgatgat gaaatcacct taggtagtga aaagttatat
    34081 gtatgtgaat taagggttgt attgattaaa gttaattctc tgtatgttga aagattgcat
    34141 aaatcagttg aagcaatggt cttagtaaaa aacttgaaaa aagatttaga taaaacactt
    34201 gttgtaaaca aaataaagaa ttttgtgcta gatgagattt gggtagaaaa tctagtaaaa
    34261 gagaatatag aagttatatt tgtagaaagc taggaataac cctagctttt ttatattaca
    34321 atctaagaga ggaaaacaat atgttaagag tatctaatgt aaaacttgga attgataaag
    34381 atatatcttt attaaaagat gttatactta agaagctaaa aataaaagaa aaaaacttag
    34441 tcaaatatag tatatataaa gaatctatag atgctagaaa aaaaggaaaa atggaatttg
    34501 tttattctgt agatgtagaa gttaaagatg aaaataaaat acttaactca aaaataaaag
    34561 atgtaacaaa aataaaggaa atcagatacg taaatgtacc tgtgggtgat aaaaaattaa
    34621 agaataatcc tttggtaatt ggtagtggac cagctggctt gtttgcaggt ctgcttttag
    34681 ctcaaatggg ttatgaacca atcatacttg aaagaggtct tgatgtagac agtagaacca
    34741 aagatataag tgatttttgg acaactagaa aattaaaaaa taattcaaac gttcaatttg
    34801 gagaaggtgg agcaggaact ttttcggatg gtaaacttac aactagaata aaagatatac
    34861 gatgtagaaa agtattagaa gagttagttc agtttggagc accagatgaa atattatact
    34921 cacataagcc acatgttgga acagatatat taaaaaatgt agttaaaaat ataagaaatg
    34981 aaataataag tttaggtggt caagttagat ttaattcaaa agtaacagat atagttgtat
    35041 caaatgattc tatacaatct gtaataataa atgatactga aaaaatagat acagatacaa
    35101 ttatattagc tgttggacat agtgcaagag atacttatga aatgttgtat tcaagaggta
    35161 taaaaatagt tcaaaaacct tttgcaatag gagctagagt tgaacatcca caagatttaa
    35221 taaataaagc acagtacaag gatttttata atcatcctag gttaggagct gcagattata
    35281 gacttataga gcatgtttca aatggaagaa cagcatatac tttttgtatg tgtccaggag
    35341 gtagtgtcat agcatctgca tcaaatgatt ttgaaatagt tacaaatggt atgagtgaac
    35401 atgcaagaga taaaataaat gcaaatagtg catttttagt aaatgtaacg ccagaagatt
    35461 ttaagagtga taatccacta gcaggtgttc atttccagca gaagtatgaa aaattagctt
    35521 ttgaattagg tggaaaaaat tataatgcac cagttcagct tgttggagat tttataaaca
    35581 ataagacttc tttgaattta ggaagtgttg agccatcata tagaccagga tatacactta
    35641 caaatttaag tgaatgtttg cctagcttcg taacagagac tatgagagaa gcgtttatat
    35701 ctcttgataa aaaattgaat gggtttgcaa tgtatgatgc tgttttgacg ggagtagaaa
    35761 ctaggtcatc atcaccaata agaatagtaa gagatgaaaa tactttagaa tcagtgagta
    35821 ttaagaatct atatccatgt ggtgaaggtg ctggatatgc aggtggtata gttacagcag
    35881 cagttgatgg tataaaatgt gcagaaaaaa taatacaaaa atacaccaat aatacaaaaa
    35941 tttaacataa acttaatgat tttttaatat ttaacatgaa tttaacatta tcactcctat
    36001 aatttgataa aatacaatag gttataaaat ttttagatac agtaatctct ttacaaggag
    36061 gaaataattt gaacattata ataagtctaa tgggtggatt aggtctgttc ttatatggta
    36121 tgaaccttat gggagaaggt cttcaaaaat ctgctgggac aaagctaaaa aagataataa
    36181 aactacttac tagtaaccta tttatgggag ttttggtagg tacaggagtt acagcagtaa
    36241 tacaaagttc aagtgctact actgtaatgg ttgttggttt cgtaaatgca ggaataatga
    36301 ctttaaaaca ggcaatagga gttataatgg gtgctaatat agggactact gttacagccc
    36361 aattggtatc tttcgactta actggtatgg ccccagtagc attaggtgta ggtattatac
    36421 tttatttatt tggaaataag cctagaataa aaaatatagc cgaaatactt ataggttttg
    36481 gtatattatt tacaggtatg gattttatga agatggcagt ggagccacta agagactatc
    36541 aaggatttac tgacttactt gttacatttg gtagatatcc actacttgga ttattattgg
    36601 gatttgggat tacagcaata atacaaagtt caagtgcatc tatgggtatg ttggttgcac
    36661 tggcagcaga aggtctagtg ccattatcag cagcactgcc aattctttat ggccaaaata
    36721 ttggtacatg tgtaacttca ctgttatcaa gtattggtgc taacaaaaat gcacgaagag
    36781 cagctatgat gcatctaatc ttcaatgttt taggaacggt gatattctta atattcttga
    36841 ataaaccagt agtaagcatg gttacaagtt gggacccaag caatgttgca agacaaatag
    36901 ccaatacaca tacattgttt aatataatat ctgtattgat acttttacca tttacaaatc
    36961 ttattataaa actagctata aagcttgttc ctgatagagc aggtgatata gatgaagatg
    37021 aaactaaaac tattaaatat atagatgata gaatgataga aactccttct atagctcttg
    37081 caaatacgat aaaagaagca cttcgtatgg gtgagaaggc gaaggaaagc ctaaatgcat
    37141 ctatggaagc tttagtagaa cattcaactg aaaaaataga taagacatat aggcgagaga
    37201 gattaatcaa tgatttacag aaagctattt taaattattt acttaaactt tcaaaagcac
    37261 ctttagatga tgattcaaga gaagttgtgg atactttgtt taatacagtc aatgatatag
    37321 aaagaattgg tgaccacgct aaaaatatag ctgaattatc gcaagttgca atagattcaa
    37381 atatttcttt ctcagaagaa ggacaatctg aacttgatgt tatgtataat agagttgttt
    37441 cagcatatac ttatgcatta gaatcaatga gaactgataa tgttgattta gcttgtaagg
    37501 ttataaaaat tgaagaacaa gttgatataa tggagaaatc ttgtagagct aatcacatgt
    37561 atagattgaa caataatcta tgtagtatag aaaatggagt tatttattta gacgtaataa
    37621 gtaatttaga gagaatatct gaccatgctg tcaatatagc acaacaagtt atagcaaaga
    37681 gattaggaaa tgattaaaaa taattaattt aaaataatat aaaatttaaa atagaggata
    37741 aataaaagag aaactttaaa tgatacgttt agagtttctt tttttgtaaa tttatttgac
    37801 taaaattttt aaatttatac atagttatag catttagaag aatcgatttc ctgataataa
    37861 atgatgagat atgtatgatt ttgtggcaaa cttttaaaaa aaacacctat attttgtgtt
    37921 ataatctctt taaaacgaat atgctgaaaa gagggattta aatgggaaaa ataaattttg
    37981 attatagtaa agctacagag tttttttgtc aaaacgaaat agatgtcatg caaccatatg
    38041 tagatgttgc gcacgacatg ttacataata aaactggttt aggaaatact tttttaggat
    38101 ggatagacct tcctaaaaat tatgataaag aagagtttga cagaataaaa aaatctgctg
    38161 ataagataaa atcagactca gatgttcttt tggtaatagg tataggaggc tcttatttag
    38221 gttcgagagc tgctatagat atggtaagtc attcttttag gaatggactt aaaaaagagc
    38281 aaagaaaggc tccagaagta tattttgtag ggcataatat aagctcaaca tatattatgg
    38341 atttattaga tataattgaa ggaaaggata tttctgtaaa tgtaatatct aaatcaggaa
    38401 ctactacaga accagctctg gcttttagaa tatttaaaga ttacttagag aaaaagtatg
    38461 gtaaggaaga agcaagaaag agaatttatg caacaacaga tgcaagtaaa ggtgcactta
    38521 gacagttagc tacagaagaa ggatatgaga cttttgtaat accagatgat gtaggtggtc
    38581 gtttttctgt attgacagca gttgggttac ttcctattgc tgctgctgga cttgatattg
    38641 atgctatgat gaaaggcgca aatgatgcta gagaagcatt tcaaaatcct gacttaaaaa
    38701 gtaatgatag ttatagatat gctgttgcta gaacaatttt acatagaaaa ggaaaagatg
    38761 tagagttatt agtgaactat gaacctcaac tacattatgt aagtgagtgg tggaaacagc
    38821 tttatggaga aagtgaagga aaagaaaata aaggtttatt ccctgcttct gtagatttct
    38881 caacagattt acattctatg ggtcaataca tacaagatgg aaaaaggctt ttatttgaga
    38941 cagtacttaa tgttgaaaac tgtaagagaa atataactat aagttcagag gaagtagact
    39001 tagatggttt aaactattta gcaggtaaga ctgtagattt tgtaaatcat aaagcttttg
    39061 aaggaactct attggctcat acagatggaa aagtacctaa tttagtaata aatatacctc
    39121 aactagatga gtataatttt ggatatttag tttatttctt tgagaaggct tgtggaataa
    39181 gtggatattt attgggagtt aatccatttg accaaccagg agtagaagct tacaagaaaa
    39241 atatgtttgc tttactaggc aaaccaggtt atgaaaaaga aaaggaagag ctagaaaaaa
    39301 gacttaaata attttaaata gttaaggttt aattaagatt aaaataatat aatgaaatca
    39361 tcgaaaagga gtttactcaa aatagatgat ttcatttttt ttatgaaaaa ttgggaaaaa
    39421 tatattttga taatttatat tatattaaga tagggagtgg tatgagatgt caagaagaaa
    39481 gaaagggata agtttagtaa tattagttgc tataataagt tcaatactta gtagttttct
    39541 aacaataatt ttagttaagg acaatttagt aagtaaatct acaggaagtt ctacgccgat
    39601 agttgtaaat gatgatggta agagtcaaaa tatttatcaa gcagttgcag aaaaagctac
    39661 gccatctgtt gtaggtatta caacaactag tgttgatact agtaatatgt ttgcaatacc
    39721 aactgaaaca caaggtgtag gaactggaat catagttgat tcaaatggat atatacttac
    39781 taattctcat gttatatcag atggtcaagc tacaagtgta aatgtattat tcaatgatgg
    39841 ttcaacaact tcaggtaaag tagtatggtt tgaccaacaa cttgatttag ctatagttaa
    39901 ggttgataaa actggactaa caccagcaga atttgctgat agtgacaaag taaaagttgg
    39961 agatatatca atagcaatag gtaatcctct tggattagat tttcaaaaaa cagttactca
    40021 agggataata agtggattag atagaactat acaaacagaa aaaactaata tgactggttt
    40081 attacaaact gatgcaagta taaatgcagg aaatagtgga ggaccattgt taaatcaaaa
    40141 aggtcaagta attggtataa acacagcaaa agcttctcaa gcagaaggat taggatttgc
    40201 aataccaatc aatacagcta agagtatagt tgaagaagta atcaaaaatg gaaaatatga
    40261 aaaagtaact ttaggcataa aaggtacaga tgtatcaaat tatgaagcag caacaggtac
    40321 aaaattaagt actgataaag gtgtttatgt tgcagaagta atatctggtt catcagcaga
    40381 aaaagctgga gtaaaggtag gggatattat aactaaagta ggagatacag atataacagg
    40441 tatgaatgat ttaaataaaa agttgtatac tttctcaaaa ggtgctagta caaaaataac
    40501 agtaaataga ggcggaaaag cagtaacaat aaatgtgaat ttctaaatat gaccagaaag
    40561 atagagacta catggtaaag tgtagtctct atttttattt aatttttata gtatatacat
    40621 ctaaattaat taaaatgatg tattgtttat agtttattgc tatattttct tataaaaata
    40681 aatttaataa atatttaaat ttaatttaca aaaacaatat aaatatcata ataaaataaa
    40741 attttttaat attactaaaa atatatagac aaaataataa cgaatatagt attatgtaat
    40801 tgtagaaatg cttttcatag gtatattcta tgaagatgaa ttgcataaaa atgaaaaaat
    40861 ggaatggata ttatttaggg gtgaaaaatc atgaatccat tagttataaa cttacaaaaa
    40921 tgtagcatac atgatggacc agggataaga agtacagtat tttttaaagg gtgtccactt
    40981 gaatgtgttt ggtgtcataa tccagagagt caaacatata caaaacaagt tttgtataat
    41041 gaagaaagat gttcaaaatg tgaagcatgt ataaatatat gccctcataa ggcaatttac
    41101 aaaggtgaaa caaaaatatg cttagaccaa gataagtgtg agttttgtga aacttgctta
    41161 gactattgtg taaacaatgc aagagagatt gttggtcaag agtattctgt aagagatttg
    41221 gttaaagaga tagagaagga tagaattttc tatgaagaat caggtggtgg agtaactcta
    41281 tcaggtggag aagtaatggc tcaagatatg gacttcattt gtggagtaat aaacatgtgc
    41341 aaatcaaagg gaatccatgt agcaattgat acatgtggct atgcaaagtc agaaaattat
    41401 gaaagagttg ctaaatgtgc agatttgttt ttatatgata ttaagcttat tgatgaagat
    41461 aagcacataa aatttacagg aaaatcaaat gacttaatac tgaaaaatgt taagatttta
    41521 agtgaattag gggctaacat aaatataaga ataccattaa ttgtaggagt aaatgtagat
    41581 gatgaaaatt tagaggttaa aaaaatgata gagtttttaa agcctttgaa tatacaagca
    41641 gtaagtcttc ttccatatca taatattggt aaacacaagt atgataaaat ttataaaaag
    41701 tatgaaggag aagaactaca aagaccatca gaagaaaaat tagaggaaat aaaaagactg
    41761 tttgaagcaa gtaatttcaa tactaagatt ggagggtaat aaaatggcaa gaggaacttt
    41821 tgagagaact aaaaaattaa gagaagaaag tattaatgca gaaccacaca tatctattga
    41881 aagagcagta cttatgacag aagcttataa aaaatatgag ggaagtgtag agattcctgt
    41941 acttcgtgct ttatcattta agcattatat tgagaataga actcttagca taaatgatgg
    42001 agaattaata gtaggtgaaa aaggtgattc tcctaatgga gcaccaacat atccagaaat
    42061 ttgttgtcat acaatggaag atttagaagt aatgcacaat agagatataa taaattttag
    42121 cgtatcagaa gaagctagaa aaatacataa agaagaaata attccttttt ggaaaaagag
    42181 acaaacaaga gataagataa taaatgcaat gacacctgag tggttagcag catatgaagc
    42241 aggtatgttt acagagttta tggaacaaag agctccagga catacagttt gtggagatac
    42301 aatatataaa aaaggatttt tagatttaaa gaaagacata gaagcaagat taaaagagct
    42361 agattttctt aatgatttag atgcatataa taaaaaagca gatttagaag ctatggcaat
    42421 agcttgtgat gcaatggtta tattaggtaa aagatatgct gagaaagcaa gacaaatggc
    42481 agaagaagaa acagatgaag ctaagaaaaa agatttatta ctaattgctg aaacttgtga
    42541 tgttgtacca gctcacaaac cagaaactta tcatcaagct atacaaatgt attggtttgt
    42601 tcatataggg gttactactg aacttaatat atgggatgct tttactccag gaagacttga
    42661 ccaacatcta aatcctttct atgaaagaga tgtagaaaat ggaatactag atagagatag
    42721 agcacaagaa ttacttgaat gtttatgggt taaatttaac aatcagccag caccaccaaa
    42781 agttggtata acattaaaag aaagtagcac atatacagat tttgcaaata taaacactgg
    42841 tggaataaat ccagatggtc aagatggtgt taatgaagtt agttatataa tacttgatgt
    42901 tatggatgaa atgaaattga tacaacctag ttcaaatgtt caaataagta aaaagactcc
    42961 tcaaaaattc ttaaagagag cttgtgaaat atcaagaaaa ggatggggtc aaccagcatt
    43021 ttataatact gaagcaatag ttcaagagct tatggaagct ggaaaaacta tagaagatgc
    43081 aagacttggt ggaaccagtg gttgtgttga aactggatgt tttggtaaag aagcatatgt
    43141 tctaactgga tatatgaaca ttccaaaaat acttgaatta acattaaata atggatatga
    43201 cccaattagt aaaaaacaaa taggtattga aactggagac cctagaaatt tccaatctta
    43261 tgaggaacta tttgaagcat ttaaaaagca attacattat atgattgaca taaaaataga
    43321 aggaaatgct gtgatagaaa atatatgtgc aaaacacatg ccttgtccac ttatgtctac
    43381 aatagttgat gattgtatag aaaaaggcaa ggattaccaa agaggtggag caagatacaa
    43441 cactagatat atacaaggtg ttggtatagg aactataaca gatagtttaa cagcaataaa
    43501 atataatgta tttgataaga aaaaattcga tatggataca ttgcttaaag cacttgatgc
    43561 taattttgaa ggatatgaag ctatacttaa tttagtttca aataagactc ctaaatatgg
    43621 aaatgatgat gattatgctg atgaaataat gcaagagata ttcaatgcat attacaatga
    43681 ggtaactggt agaccaactg tttgtggtgg agaatacaga gtagatatgt taccaacaac
    43741 ttgtcatata tactttggag aaattatggg agcaagtcca aatggtagac tttgtgcaaa
    43801 accagtttca gagggaattt cacctgaaaa aggtggagat actaatggac ctacagcagt
    43861 tataaaatct tgtgctaaga tggaccacat aaaaactggt ggaacattat taaatcaaag
    43921 atttgcacca tcagtagttc aaggagaaaa agggttagat aatatggcta atctagtaag
    43981 agcatacttt aatatggatg gtcatcatat acaatttaat gtatttgata aaaatgtact
    44041 gttagaggct caaaagaatc ctcaggatta taaagactta atagttagag ttgcaggata
    44101 tagtgaccat ttcaataatt taagtagaac actacaagat gagataatag gaagaactga
    44161 gcaaacattc taggtttgct tgtaaataaa acttcttact atataaaatc gtaactgata
    44221 taataaagta taaatggttt gattccattt ttaaaatttt agattaatta tgagctaact
    44281 ttctaaatag gaagttagct cgttaagttt aatgaaaaaa tcgttaaatt tatatttagt
    44341 aaatcataca catattagta agtaatacat acaaaagaat aatttattat agttataata
    44401 aggttatatt ttataaaaat aagataattt taagcagtta aaataaagtc aatttagcta
    44461 ctttaaaata aaactaatag aaaaaaggag atttgatatg aaaactttag gatttattgg
    44521 ttcagggaat atgggaagtg caatgatagg tggcatagta cagtcaggat tagtaaaggc
    44581 agaagatata actgtatcag atttaagcca agttgcctta gataaagtaa aagaaaaatt
    44641 tggagtaaat actacaactg attcaaaggt agctgttaca aattctgatg tggtaatagt
    44701 agcacttaaa cctaatatat gcgaaaaggc tttaactcca cttaaagaat taatagatgc
    44761 aaataaaata atagtatcta tagcagcagg taaaactata gaaagtctgg aaagttttat
    44821 aggttcagat aaaaaaatag taagagtaat gcctaatact ccagcccttg ttggcgaagg
    44881 aatgtcagct ctttgtaaaa atagtaatgt aacagatgaa gagttaaata tgattaaggc
    44941 tctgtttgaa tcttttggtg aggcagaaat agtatcagaa tatttaatgg atacagttac
    45001 aggtattagt ggttcttctc ctgcatatgt atttatgttt atagaagcta tggcagatgc
    45061 agcagtatta gctggaatgc caagacaaca agcttataaa tttgcatctc aagcagtaat
    45121 ggggtcagct aagatggttc ttgaaactgg aaaacatcca ggtgaattaa aagatatggt
    45181 ttgttctcca gcaggaacta ctatagaagc tgttaaggta ttagaagaag aaggctttag
    45241 agcagcagtt ataaaatcta ttgttgcttg tatagataag tctaaagata tgagcaaata
    45301 acagatgtaa ttaagtttac tatatggttt cttgaattgt ttttttagtg cttatttata
    45361 tctatacata aatgtatttg gtataagtaa aataagagta atttactata aaaaggaatt
    45421 taatgattga gtgataattt attacttaaa tattaataat taaatagata tatttttaac
    45481 tatatttgag atatatcatt gtgaaaaaat aaaatttata agtattattg tgaggttata
    45541 agtagattta tttaaaaata aatttattta tagccttttt aaatttgaaa taatattcat
    45601 aaggtaataa aagttacaaa tattttcttc gctaataagt tttaaaatac ctataatgtt
    45661 ttacttttta ttataataaa tacaataaat atattcttat ataaattcta tgtcagatat
    45721 atggaaagat aagaaccttg agattatgtt aaaaaatcta gattttatac ttataaaaag
    45781 tttgatttta caaacttaca aaagagaatc aagataggaa tataattatc tttttcataa
    45841 atatataaat gatatactaa tattatagta ttattgatat ttgtattgga ggttgaacat
    45901 gagggatgaa attttaagtt ttgtaaatga atgtgtatta tttaacaact ttgatgaact
    45961 taaaaaagaa agtggcataa taacagaaga tatagtaaaa aagtttaatt taagtagaac
    46021 tcaatcaagt agattattaa atgatttagt aaaatctgat aatcttataa agataaacag
    46081 tagacctgtt atttttttac ctaaaaataa aataaatgat gtagtaggac ctacaaaaaa
    46141 aagtatttat gaaaatttag atgaacttaa aaatgagtgt aaattattaa agcaagagac
    46201 tgtgtttaat agtatcatag gatatgataa tagcttaaaa gaagtaatcg aacaaataaa
    46261 aaccgctgta ctttatccaa atggcggact tacaataatg ctgacaggtg aaagtggagt
    46321 aggaaaaact tttttagcca agataatata caaatatagt gtaagcagta atgtattaga
    46381 aaaaaattct ccatacaatg tgttaaactg tgcgcagtat tataataatt tggagttatt
    46441 gtctagtcta ttatttgggc atgtaaaagg agcatatact ggagcaaatg aatctagagc
    46501 aggtctaata gaacaatcag atggtggtgt tttattttta gatgaagtac atagattaaa
    46561 tagtgaaggt caggaaaagt tatttacatt tatggataca ggtacgtttt ctaggattgg
    46621 agagaatgga gtaattagaa aagctagggt aagattaata tttgctacaa cagaaagtct
    46681 atctgaattt ttacaaacct ttttaagaag aatacccata aatatttatg ttcccaatat
    46741 agaagaaaga gggactattg aaaaaagaaa gattgtagaa cattttattt atgaagaaag
    46801 taaggtactt ggtttgccta tagagataac tcaaattaca ttaaatgcga ttttaaagat
    46861 aaaattcaag gggaatatag gtgaatgtaa aaatgtaata aaatatgcat gtggacgagc
    46921 ttatgcaaaa aatcaaagaa gaaatggaaa tatttttgtt actcttcaag accttcctag
    46981 aaatctttat atagaaaatg tagatatatt tcaattccaa agtggtaaaa gcaaaagcat
    47041 tatcttttct cctaaacaat ttagtattga aatgtcaaat ggaagaaata aaagaaatat
    47101 aatctatcaa agtttcataa aatgtttaga ttactttgat agactagaaa aaaatgaaat
    47161 aacaaaggaa aaatttagta aaaagagttc taatctaatg attagactaa tggacaattt
    47221 agtcttttca aataatgatg aaaataataa cgtacttatg gagcttataa tatcgataat
    47281 gcaggatata tttagaaatt tagaagttaa taacaatata aaatatgatg ggaataatgt
    47341 attggcattt tcgtcttatc tttatttgaa ggacgaatct tatcaattgt ctgatgagta
    47401 taaagcttta gaaaataaat tactaatata tttaaaaaaa gagagttaca aagaatactc
    47461 tataataagt aaaatatcaa cattaataac gaatagattg gatatgtatc ttaataatgt
    47521 agatataatc ataatgatta tatttattaa atcaacaatt ataagaatga aatttaatca
    47581 agttaatgcg actattatag cacatggata tgctactgcc agtagtatgg cgaatgtatg
    47641 taatcggatg ttgggtaaaa atatatttac agctattgat atgcctatag atgctactat
    47701 tgcggatgta tcaaaacaaa tgtctagata cctagaggga gataggataa agaatggttt
    47761 aattgtgtta tttgatatgg gctctcttaa tcttatatat gaagatttaa agaataagat
    47821 aaatagccca atgttattta tagaccaaat atccactatt tctgcactag aagtaggaaa
    47881 tttattaatg caaggtaaaa acattgaaga gattgcagaa attatggatg aagaaactaa
    47941 acctaatata caattgtatt atcctcaaaa aaataaagaa tatgctataa tatcatcttg
    48001 ttttacaggg ataggtactg ctataaaaat ccaacatcta ctttccgaaa gtttaattgg
    48061 aatagttgat gtcaaaataa taccttatga ttatgatagt cttgtttcta tgggagataa
    48121 agaaccagtt tttgaaatgt atgatgtgat ggctatagta ggaactgcca atccaaatgt
    48181 gaatggggta gattttatat tattagagga tataatatca ggaaaaggag aagatgatgt
    48241 ttttagaata tttagcagag tagtagaaaa tgaaaaaata aagaacataa atgattctat
    48301 tgtaaagaat ttttcattaa ttagagtaat agatagtcta actattttag attcaaaaaa
    48361 gataatagag agaatagaag aaggaattaa tgctatagaa aatattcaaa agaaaaaact
    48421 ttcaaatgat aaaaagattt ctttatatgt tcacttaagt tgtatgattg aaagattagt
    48481 tagacaaact gaaattgaag agtatactga tatggatttg ttaatcaaaa accatcatag
    48541 tgaagtaaaa ctaataaaaa atgcatttag tgtgctagaa aagagttata gtgtgcaaat
    48601 accaatttca gagataggat atatttataa tattatctat ggtctaccac agtaaaatac
    48661 aagattacaa aataagtgaa tgaaatttaa aaaagtatgc cagtatttgg catacttttt
    48721 gcttttatag ataaatgaga gtataaacaa taaaaatttt aatgtatgat ggaggatttg
    48781 gggaaaatgg tatctttgat gagaattgat gacagattga tacatggtca ggttgcatat
    48841 ggatgggcaa catcactagg tataaatgta atactagttg caaatgatga agcaaaaaat
    48901 gaccaaatga aagctatgtc actgaactta gcgaaaccta gcaatgtaac tttatatata
    48961 aggggggtag aggaatctgg agaaatagta gaaaaatttg cttcttcaaa gaaaagcaat
    49021 gtattagtgt tagtaaaaaa taccaaagat gctttagttg tagctaaaaa ttcaggagga
    49081 gtattaaaag aaataaatgt aggaggatta agatatgagg aagggaagaa gaaacttaca
    49141 gatttagttg ctgttgatga tgatgatata aataacttta aacaaatagg tgatttaggg
    49201 attgatatag aatttagaat gttacctaga gacaaaaaga aacaactatc agatttaata
    49261 aaataattta ggagaaagag aatgaaaaaa tttctaattg caacacatgg agaattgtcc
    49321 agagagcttg ttgacacaag taaattaata attggttcat taactaatat tgagtatttt
    49381 tgtatgacaa cggataaatc agcagatgat gctgagaaag aaattagaag tatattatca
    49441 aataataaag atagtgaaga atttgttgtc ttaacagatg tttttggtgg aagtgtggca
    49501 aacatatgta caaacttact tttacaaggg ttcaaatttg aattattgac aggagtaaat
    49561 ttaccaatgc ttttatctat acttttatta gttgaggagg atacaaaact aatagttaaa
    49621 tcaggattag aggaagcaaa aaatggaatt gtttatgtaa atgagttatt agaaaatcaa
    49681 aacaaaatca aaaatgtaat gtaatttaat gtaaattaaa ctaagttaaa atggaggttc
    49741 aatatgttag tacaagctac tatgttagct attatagcag gagttggtat actagatggg
    49801 cgtatatttg gtcaatctat gcttgatagg ccaatagtta caggtatgtt agtaggatta
    49861 gttttaggag acataaaatc aggaattatg attggtgcac agcttgaatt aatttggatg
    49921 ggaattgcag gaataggtgc agctactcct ccagatgtag taacaggagg tgtattaggt
    49981 acagcttttg ctatattatc aggtaatggt gcagaagttg ctttagcagt agcagtacca
    50041 gtagcagtct tagcacaatc tcttggagta ttagttagaa taataaattc gtacttttct
    50101 caaaaggcag ttttctatgc taaacaagct gattttaaga aagttaccat aatgatgtgg
    50161 ttgccagtaa tattattctt tttaagtaca tcagttccaa cctttttagc aattatgatt
    50221 ggtgctgata aagttacagc attgataaat gcaataccta aagttatatt agacggactg
    50281 ggtgttgcag gaacattgtt gccagcagta ggttttgcat tgcttatgga tatgttactt
    50341 tcaaaaaaga tgtctgtatt tttctttata gggtttttgt tagcatctta tggtggatta
    50401 gatataactg ctatagcttt atttggagta tgtgtagcat ttgtactaaa cttttatatc
    50461 aataacaaag aaaataatca aaatcctcaa ttagcaatca atactttaac ggaaggagag
    50521 attgactttg aataaattaa caaaaaaaga attaagaagt atgttttggc gttcatttgc
    50581 tcttcaaggt gcatttaatt acgaaagaat gcaaaactta ggttactgtt attcaatgtt
    50641 acctgcaata aagaagctat acaataagaa agaaaatcaa gcaaaagcaa tagaaaggca
    50701 tttggagatt tttaatacaa cgacagtagt agttcctgct atattaggta taacagcagc
    50761 tatggaagaa gagaatgcta ataatccaga atttgatgaa agttcaatta gtgctgttaa
    50821 aacagctctt atgggacctc ttgcaggaat tggtgattca ttattctggg gaacatttag
    50881 gattatagca gcaggagtag gagtatcact tgcaaaagaa ggtaatatct ttggaccact
    50941 attattctta ctattataca atataccagc atttgccctt agaatatttg gacttaagta
    51001 tggttatcaa gtaggggtaa attctcttga aagaatacaa agagagggac ttatggagaa
    51061 aataatgtct atggctacaa ctgttggttt attcgttgta ggaggaatgg ttgcaactat
    51121 gttgagtata actactcctc tgaaattcaa cttaaatggt gcagaagtaa ttttacaaga
    51181 tatactagat aaaataatac ctaatatgtt acctttaacc tttgcatttg taatatacta
    51241 catgttaaag agaaaggtaa gtgtaacaaa attaactata ggcactatag taacaggaat
    51301 cgctttacat gctataggtt tgttatagaa attaattata atttattgaa tatatagata
    51361 aggaaggtgt ataaaatgac aatacaagat tatatgttag aaacaccagt tagaatgaga
    51421 gaaattatat ctaatgcaga tagcttattt aatgaagtta agagaacaaa tctaaaaaag
    51481 attataataa caggttcagg tactagctat cactcaggag tgcaagtaca accatattta
    51541 caaaatttat tagatataga tgttgtgaaa atgtatccat ttatgataac tgaagataca
    51601 ttcaagtttg ataatgaaaa taccctagta gtaggtgtat ctcaaggtgg gagtagttat
    51661 tctacataca acgctatgaa gttagctgaa gataagggct gtaaaatagc atctatggca
    51721 ggttgtaaga atgctttaat agatgagatt tctgattata tattaacagt aaattgtggt
    51781 gaagaaaaat ctggagctaa aacaaaagga tattattgta caaagttaaa cttaatgtta
    51841 ttaggtcttc aaatagctag agaaaaaggt ataatatcta gtgaaaagta caatgaggaa
    51901 ataaataaga tattagacgc aattaataga tttgaagcag tgtataagct ctctaaacaa
    51961 tggattgaaa gaaacaaaga aaagttagtt aattctaaag aaataagaat aataggtcac
    52021 tctgatatat atggagatac attagaagca gcattaaaat tattggaaac aatgagaata
    52081 cctgtaactg ggtatgagtt tgaagagttt atacatggta tatataatgc tataaatagt
    52141 gattctacta tatttatact agatacagga aaagaaccta gagtaactaa gatgattgat
    52201 gtactttctg gttggacgga aaatgtattt gctataggta gagatgttac agaaaatgat
    52261 aaaaatttaa agattgatat cacagataat ccttactatc aaacatttaa ttttatagta
    52321 cctatacaac taatatgtgg ggaaatacct acattaagag gtgtagaccc aagtgttcca
    52381 aaagatacta ggttccacat gaagttaggt agcaagaaac tgaataagta acaaaaaaag
    52441 aggttgtctc aaaatagaga taagcctctt ttttactaga taagctcttt tttactgttg
    52501 aaatgatgaa aatatatttg attttctata aaaaagagtg cctcatgaac taaaaagtta
    52561 attttgagac acccactttt atcttatttt aaactaaaat atttatatat tagtgtgcat
    52621 tatttttttt attgctgtct tccttacttg agcaagaatt agcacttgga catccagaac
    52681 aaatagaaca tccactacag acatttccgt tttttgtaga cttgaattta ttaaacagaa
    52741 taaagagtgc aaggactata attattccac ctatcaaagc ttctattatt gaattaccca
    52801 taaatagtac tccacctata cttttaacta taaaagccat tatccatgct agtataaatt
    52861 gatatgcaaa tgatgtaaac atcattttat tgccatattc ttttttcata gttgcaagtg
    52921 cagctataca tggagtatat aaagcagtaa atatcaagaa tgtatatgca gtaactccat
    52981 taaatattgt tggtaagaca acagttagat taccatatag tatatttaaa gagtttacta
    53041 cgatttcttt tgctccaaga ccacttaaaa tagcaactga agttctccaa tcatcaaaac
    53101 caagaggttt gaatatagga gatataatat gacctagagt agccaagaag cttacattta
    53161 tatcttctgt atatccaaaa acattgaatg aagaaagtcc ccaaattacg actgacattg
    53221 caaacatgac agtaacaact cttatcaaga aaccttttga tttattccaa gtatttttaa
    53281 taagtgcatc tatagtaggg attctatatt ctggtaattc taatataaat ggttcagcct
    53341 ctgttttaaa ggctgtttta tttaagaata atgcaactac aattgccata actataccca
    53401 ccaaatacag tgatgtagtt actaaagcag cattatgtgg aaagaatata gctacaaata
    53461 gtgcatatat aggtagcttt gctccacaag tcataagtgg tgcaataaga gctgtaactc
    53521 ttctatcttt ttcgctttcc aatgttctag ttgccattat agctggagaa gaacatccca
    53581 tacccataac tataggtata aaagctttac cagatagacc aacctttctc ataactttat
    53641 ccattaaaaa tgctgttctt gacatatatc cactatcttc aaacaacgat ataccaaaga
    53701 ataatgtaaa tattaaaggg aagaatggta aagtaccacc aacacctcca attattccat
    53761 caactataag tgatttaaac catgggctag agttagataa catatcacta acaggtgcag
    53821 aaatataagc ctctataaga ctagcaaatc cctcttgtag tggtcctccg acccagtcaa
    53881 aggtaaattt aaataagagg aacaatacac caataaatat cggatatgct aatataggat
    53941 tcaagactat attgtctatc ttatcactga tagatttctt ttgtgtatat gtagtttttg
    54001 tacatctcga taaaatggat tctaattttt tatatgtttc tgtttcactt ccaaagtttt
    54061 tctgatatat aaaagctttt tgcgtagctc ccttaatctc ttcttctatc ctatcaacac
    54121 catttttctt ttttgctata attggaacaa caattactcc aagttcttca cttaattttt
    54181 tggcatctat attaactcct ttagattctg caatgtctaa catattaagc aatataacta
    54241 taggcttgtt aaattgcata agttgagttg ttaagtaaag gtttcttgat aaattagaag
    54301 catcaactac atttactatt acatcaacat cttcattttc taaatatgat ttagaaacct
    54361 tttcttcatt tgagaaagta tccattgcat atataccagg taaatcgaca acctttatct
    54421 cttttccaag aaatccttct tttttttcta tagtaacacc aggccagtta cctacatatt
    54481 gatttgaacc tgtaagtaga ttaaaaacag tagttttacc gacatttggg tttcctaata
    54541 atgctacatt aatcacgggt agctcctcct ctattattgt aaagaaatat ttttagcatc
    54601 agattttcta atcgcaaggt caaagcctct aaatctaatt ataattgggt ctccaagtgg
    54661 ggcaaccttt ttaacttcta tttcagtact atttatacat ccaagtgcaa gcaatctttt
    54721 agataattta atatctccat ctatactatt aataaatcct ttttctccta tttttaaatc
    54781 gtaaacagtc attattaaca cctcctaatg aaattcatta tcattaaaat tgtaacaagt
    54841 atgatacaaa aagtcaagat ttatagaggt aattgcacat tgaaataact aatatttatt
    54901 ctaaatagat taaaaatatt ttagtctaaa ttaggtgttt ttatatattt tgtattataa
    54961 tgtaagatga tagataaaat ttggaggagt taacttaatg aaaactagaa aaatgacatt
    55021 tttaggtctt atgatagggt atagtttagc tttatatgta ttagagacat atataccaaa
    55081 tcctcttata gttatatttc caggggcaaa attgggtttg agcaatatta taactttaat
    55141 ttcattaatg ttgttaggtg tcaaagatac tgttataatt gttactatca gggtaatttt
    55201 gtcatcaata tttgcaggcc ctttatcata tctactattt agtataggag gagcttatct
    55261 gagtctttta tttatgtatt tagcgagtaa aataaaagat ttatcactaa tagggataag
    55321 cattataggt gcaatagggc ataatatagg tcaattaata gttgcaagtt taatcataga
    55381 aaatatactt gctatagggt acttaccatt tatgttaatg gcatcattag taactggtat
    55441 gtttgtaggc ttagtatcca ggtatacaat acctaaatta aagcattttt atagtaagtt
    55501 ttattgacat aatattgaca aaatataata ttgtttgata aaatgtatat aaaaatatac
    55561 aaatttgtaa tagtggtgat tattatggcg aacaatgaat tagaagcgtt aaaaaatgag
    55621 attgaagcag ttagggaaga gataaataca tacatagaat atccagaaat atttcaagaa
    55681 gagttagtag aatcaagtaa gaaaatagat atattaataa ataaatacat gtacctaagc
    55741 aaatagccca cagttgtggg tttttttgta tcaaaaatta acagatggct atattttaca
    55801 tatattaata ataatattaa gatataaaat agtatttctg atgtgtaaat tatctgtagg
    55861 acataagttg taattaagtc aggagggttt tatattatga ttgatatagg aagtaaggag
    55921 atagcaataa ggttactatt agcactaata ataggaggaa taataggctt agagagagaa
    55981 aaaataagac agtttgcagg atttaggaca catatactaa ttgcaatagg ttcttgtatt
    56041 gtttcaataa cttcgattca attattcttt gattataatt tgtatacaaa tgctgaccca
    56101 gcaagaatgc ctgctcaagt attatctgga attggttttt taggtgcagg agcaatttta
    56161 aaaaataaag gtggagtaaa agggcttact acagctgctg ggatgtgggc aactgcatgt
    56221 attggtatag cagttggata tggttattat gaattggcaa ttctaggtgg attatttgta
    56281 atgatagcat tatttgtgct taggataatc aatacgtttt tatttagcca agaaaagaat
    56341 gtattgatat taaaattaag caatttggat tctatacctt ttttatatga taaatttgaa
    56401 aaaaatcaac ttatcgtaag aaatatggaa atagaatgtc gtaatgaaga atactggaag
    56461 gttaattttt ttgtatctta tgctaagaga atacacttac acgacgttat gaaagaaata
    56521 aatatgatag aaggagtaat aaatgtagat tatttggact aaaatatata caatatattt
    56581 tgtaaagtga ataaaatata aaacaaatta aaatagaggt gtatgatatg ggaactgata
    56641 gaagtacaat agaagaaaag tacaaatgga agatagataa gatgtactcc agtaaagagg
    56701 aaattgaaaa agacataagt aaagtaaaaa atcttatcca agaagtaaaa gaatataaag
    56761 ggaagctttc tgagagtaag gagaacttat ataaagtatt aaatatctct gaaaatgcat
    56821 ctaggattgt tcaaaattta tatgtatata ctcatatgaa acaacatgaa gatactagaa
    56881 ttaatgataa tcaaggaaga gccactaaga cagaaatgtt atctactgat ttaggtgtgg
    56941 ctacttctta tatagttcca gaaatactgg cgatagaaga gaataagtta agtgaatact
    57001 taaaagatga gaagctatcc ttttatgcaa aatatataaa ggatatattg agagataaac
    57061 cacatacttt gagtgaacga gaggaagaaa tacttgcagc tacatcagaa ttatcgacaa
    57121 ttccagaaaa tgtatacgat atgttagcat atgcagatat ggaatttcct gagatagaag
    57181 atgaggaggg gaataaggtt aagctttctc attcaaacta ctctttattt ataaaaagta
    57241 agaatagtag agttagaaaa gacgcttttg aaggtgaatt ttcaacatat gagaaatata
    57301 aaaatacatt tgcctcaacg ctttatggtg gaataaaatc agagatattt tacgcaaaaa
    57361 ctagaaagta taattcagca atagaagcgt ctttattttc agatgatgta agtttagatg
    57421 tttacaataa tcttatatca gctatagata aaagtattcc taatctaaat aaatatgttg
    57481 agcttaaaaa gaaattttta ggattgaatg aaatacacat gtatgatttg tatgttcctt
    57541 tgacagataa atttgacatg gatataccat atgataaagc acaggatatc atattagagg
    57601 cacttaaacc tttaggagaa gaatacttga aggtcataaa aagtgctttt gatgaagggt
    57661 ggattgatgt atatgataat gaaggtaaaa aaggaggagc ttattcttgg ggaagttatg
    57721 attcacatcc ttatatatta atgagctata aaaatgacct taattctcta ttcactttaa
    57781 ttcatgaatt agggcactct gttcacagtc attattcaag aacaagtcaa ccatttttat
    57841 attcggacta taagatattc gttgcagaag ttgcatctac attaaatgaa ttacttctta
    57901 taaattattt attagaaaat tctaagtcta aggatgaaac tatatactta ttaaattatt
    57961 atttagaaca atttagaact acagttcata gacaaactat gtttgctgaa tttgaaaaaa
    58021 ttgtacatca aagggtagag agtggagaac cacttactgc tgatgaattt actaatatat
    58081 actacaaatt aaatgaaaag tattacggaa aatctgcaat agtagataaa caaataggaa
    58141 tagaatgggc gagaatacct catttttatt caaacttcta tgtatataag tatgccacag
    58201 ggttttctgc tgcaagtgct ttaagtcaac agatactttc agaaggaagt actgcagtag
    58261 atagatatat aaacttctta aaaagtggag ggtcagaata cccactagaa caattaaaat
    58321 ctgctggtgt agatatgacg aaaaaagaat ctatagagga agccttaaat gtatttgctg
    58381 aattagttaa taaactagaa aaagaattat aggatgtgat aggttgatag cttcaatgaa
    58441 gacttctgat aatatgagtt gtattatgtt tatatctgcc taaagtaact ttaaaaagtt
    58501 aatctaatta agaataaatc atataaaatt aagtttttac atgtatttaa caatatttac
    58561 atttacttaa ccctgctcta atattcattt aacaaagaat ctgtattatt aaaatataga
    58621 aaaatgacaa caaaaaatta aaatataagc gggggtaagt aaaatgttta aaaagaaaat
    58681 aggcgttctt ttagctagta caatgttagt tggagcttta gcagtaggat gtagttctaa
    58741 ctcaggaggt tcaggtgatt caggagattc aagcaaagtt tctgtttcag gttcaacttc
    58801 aataggtcct cttatggaaa ctatcggaga aaagtttcaa gaaaaaaatc aaggtgttag
    58861 tgtagaagta caacagattg gttcttctgg aggtataaaa aatgctatag aaggaacatc
    58921 tgaaatagga atgagttcaa gagatttaaa agatgaagaa aaaagtgctg gattaaagga
    58981 aactaaaata gcaatagatg gtatagcatt gataactaat actgcaaact cagtaaaaaa
    59041 tctgacaatg gaccaagtta aaggtatata tacaggaaag attactaact ggaaagaagt
    59101 aggaggaaat gatgcaccaa tagtaatagt atcaagagaa gaaggttcag gaactagaga
    59161 tggattccaa gaaattgtag ggtttgattc agaagaacta actaaagatg cacaaataag
    59221 tgatggttca ggaaatataa aaactacagt tgaaggaaat gaaaatgcta taggatatat
    59281 atcatttgga tatgttgatg agaaagtaaa tagtttaaaa ataaatgatg ttgaagcaac
    59341 tgatgcaaat gtaatagcta ataaatatcc aatatcaaga ccatttatat tagttaataa
    59401 agaagatggt ttgtcagaaa aaggtaaagc tttaatagat tatatattaa gtgatgaagg
    59461 acaagcactt gtttcagaaa aaggatttat taaagttaat caataacaag agttataata
    59521 taaagtaaac atataatacc ctgtgagata agctcataaa aatgatgctt taacttgcag
    59581 ggtattgtct attattgaat gtagaaatat aaatatcttt agataaaata ggtattttag
    59641 tatcttatct ctaagaatat ggaaatattg acaaaataat attacaatgt tagaataaaa
    59701 aagaaattta tgaaaatata taaaagattt ttaataaaaa tattttagaa actgagaagt
    59761 atatcttatt attgggcatc tagagatata tggagttagt ggtgcaaccg gctatgaata
    59821 taatttttat attgcatagt ctttttttgt tttaaaatta acaaatatgt tgcaaaattt
    59881 gaaaataaga gtattagtcg ttaagatttt tattgatagg taaaattttg gcttttaaag
    59941 tagccatttg ttggaaaagg gaaatttttt taaaagaata atagtattat aataagaaaa
    60001 taaagttttt ataaacattg agatgcagaa tataaacctt ttgtaacaat atatttacca
    60061 aaataataca atttatttta tattataacc atataaatca tataaatcaa tactcaaact
    60121 agccaataga caagtttcta gaaaaataat aaatttatac gtgaaaatga ctttaagctc
    60181 cattctccaa caatcggaga ctttaattag tatttaataa tgaatcttaa tgtttaatac
    60241 aatattcaag aattattcaa aaatacttaa gtattatagt ctagtttgta aagatattat
    60301 aaaataataa taccagttat atatgaaaat aaaacattat aatttgtaat aactataaaa
    60361 cttggaatta taattaaaat acctttgaat ggagtggtgt aatgtataat ttactggttg
    60421 tagatgatga tgttgatatt ttaaacataa atcaaattta tttttctaat ataggtttta
    60481 atgtatatac atcagagact gctaaagatg ctatggatat agtagagagt atagttttag
    60541 actgtataat tttggatatt tcattgccag atatagatgg atataaaatg tgcaatgata
    60601 taaaagaaaa ggttaacata cctataatat ttttatctaa ttatgagcat gaagaagaaa
    60661 aagttcgggg atttttagct gggggggatg attatataac aaaaccttat agtttaaaag
    60721 aacttgaact tagaatatat gctcgtatga ggcagtacaa aaatattaca tcagttccta
    60781 atgtgttaaa gttttcagcg ttagtaataa atgtaaattc aagaaaagta acttttgatg
    60841 gagaaagtat agatttaact actatggaat ttgaaatatt attgtttcta gctgaaaaca
    60901 aagaacaagt attttcgaag atagagatat ataaccaagt ttggaaaatg cctgatattg
    60961 gagatcaaca tacagtgcag gttcatattg cacagatgag aaaaaaaatt aattctttaa
    61021 gcagggagca tcaatacatt caaaccgtat ggggaaaagg atataagttt gtaccataag
    61081 tgagatactg taattaataa atagttcttg ttttatttaa tactactatg aaagagatgt
    61141 ggttgataca atgaaaaagt ataatatact aaaaggaact ctcattgtat tgatagtctc
    61201 tctgttggca ataacaggat taaatcttct gtataaatat gataataaat atacatataa
    61261 gtctattaga gcaagtaatg gaattttaac tatagataat gatactttca ctaaaggtaa
    61321 acttgtcttt ttgatagatg actgggagtt ttataatcat aaaatattta aaccgaatga
    61381 ttttacagag caagatataa aaccagaata tgtttttata ggtcaatatc cagatttttc
    61441 tatgagaaag gggactcagt caccatatgg gaatgctact tatcgtatga gaataaaaaa
    61501 tgaaggcaag gataagctac taagtttaga gcttcctgag attttttccg caagtgaagt
    61561 atggataaat ggagaaatgg tttctaaatt aggagatgta ggaacagaaa aatatagacc
    61621 caaaattaaa aattcagtag taagttttat tgcaaagaaa aatacagaaa ttataatcaa
    61681 tgtatcgaat ttttcacatt attatagtgg tttgtattat ccgcctgcat taggagaaac
    61741 aaaggacata agtaatatga tgttttatcg attacttttt tatagtataa tttgcttcac
    61801 aacttttgca atagctatat tttcattgtc tgtatggttt ctttcaaaca aaagtaaaat
    61861 ttacttatat tttggatgta tgtgtgtttt ctttgccatc catgtatcct atccctttgt
    61921 acatttgtta ggactgcctt tggtaagctt tacatatgcg gttgaagatt tgtcatattt
    61981 tatggtagtt ttatgtatta tttgtataaa tggaagtata tccaaaatag aagaaaataa
    62041 agtgtacaga tttttaattt taccattagg aattacaatg agtatcatat ctatagtgat
    62101 accaattatt cttcttccat atgatatgta ctttgtcaac atatatggga agttaataga
    62161 catatataaa tatattgtat ttatatatat attaatgtct tcgctggttt caatatataa
    62221 aaagacatgc tctgagcata taagttctta tattttagtt tcagtaaaca ctgtttttgg
    62281 aattagcata ttatttgatg cagttacgag taatagattt gagcctatat ttacagggtg
    62341 gcaaacagaa tattgtgggt ttgtaattgt tattttattt tctgttatga tgataaacag
    62401 gaatagaata ataataaaag agaatgaaaa acttacaagg aatcttgaac atgaagttga
    62461 agtgagaact aatgaactta caacattgtt aaatgaacgt aaaagattct tggcagatgt
    62521 agcacatgat ttaaaggcac ctgtttcggc tatacaagca tttatagatt tgataaaggt
    62581 tggtaatatt catgtagatg aagagacaag aacctatctt tatgctataa atcaaaagtc
    62641 aaatgaagta caaaatagag tacgtagttt gcaagaattt acatcacaag ataaaagcat
    62701 tggtattagt gaaaaaatat gtctgaataa ttttattaaa gagatatatg aaaataattt
    62761 gccagataca caagcatgtg gtgtaaactt tgaaacttac gtattggaga aaaaaatata
    62821 tgcaaagata aataaagaga tgcttacaag agcatttgag aatttaattt ataatgcttt
    62881 gacctttact ccatttgatg gaaagataat tttatcaatg gataaagaag atggatttgc
    62941 aattattaaa atttctgata atggaaaagg tatacctcca gaaaatatac caaaggtatt
    63001 tgacagattc tttacagaaa gagacaataa tgagccaaaa gggcaagggt tagggctata
    63061 tattgtcaaa tccattataa gagagcatgg tggagaaatc tttgtagatt cagaactagg
    63121 aaaaggaact gtttttacaa taaaattaag tctatatttt ttataataat agcttattta
    63181 atataaaaga ataatgatat ttgggagtga ttataatgca aactaagata ttgataatag
    63241 atggagacaa ggataattgc aagaagctaa aaggatttct ggaggaaaaa ggaataagta
    63301 tagatttagc atataattgt gaagaagcta ttggaaaaat atttagcaat aaatatgatt
    63361 taatattttt agaaataatt ctaagtgatg gtgatggatg gactctttgt aaaaaaatta
    63421 gaaatgtaac tacttgtcca attgtttata tgacttatat aaatgaagac caaagcattt
    63481 taaatgcttt aaattctggt ggagacgatt acttaataaa gcctctaaat ttagaaatat
    63541 tatacgcaaa agtaaaagct attttaagaa gaatgaactc atatgtaaat aataatgaaa
    63601 ataatacaaa attagaggaa tataatagaa ctagtggagt aataaagtta gaagataaaa
    63661 taataaaatt gactccaaca gaaaataagt tgttaaactt ttttatagag aatcctgaaa
    63721 agactttgac tactaaagag atatatgaac atgtatggat gaatgaatac ttagaggata
    63781 actatagtgt ggtagttgca gtgaatggat taagaaagaa aatagagaaa gattataaaa
    63841 atcctcagaa aattataaca ataagaggag caggatatta ttttaataaa gaataataaa
    63901 tacatataaa caaacataaa taagtattaa aacgtgcagt tttagcggta tttaaaagaa
    63961 attattataa actaactgac tgacatttaa ctgacaaaac ctattttttt aggttcaact
    64021 gacatttaac tgacagtcag tttttattat ttaaatagaa gtttacttaa attatcagaa
    64081 gcttctttgt ccatttcctc taacacatga gagtacctat tcatagttat ttttatatct
    64141 gtatgaccta atctttctga aacaactttc atatttgtac ctcctagcaa aagcaaactt
    64201 gcgtttgtgt gtcttaaatc atgtactcta atatttctta aattatttct tttaataaag
    64261 ttatgaaaag ttttacttaa tgcagttggt atccaagcat taaaatttgt atttagacat
    64321 actaagttat tttcatttat aagtgtacct tgtaatttca ttttgttttg ttttaatttt
    64381 tctattttta gtttttcaac taactctttt ggaacagata acgctctttt tgattttgca
    64441 gtttttggtt ctttaaaagt tattttgcta tttgcatata tgagaatttg attaatattt
    64501 attatactat tatctaaatt aacatcagac catctaagac cagctacctc tccaattctt
    64561 aaacctaaca gcaacattaa gagaatgggt atctctatca aactatcttt caacttctct
    64621 attaacaata gcgtttcttc tctattatat atttcaattt catatgtaac attttgagct
    64681 ggcaatttta caaaatcgca aggattttca gatatttctt ttagcctata agcttctttt
    64741 aaaacagaag atagaaaatt atatctaact tttgcagttg atggtgcaga accatcaaaa
    64801 tttttatcta taaagatttg aagaaggcta gggtttacat ctatcaatat tgtatcttta
    64861 aaaaaaggtt ctatataatt cttaacccaa gatttcctat ttataactgt ataaggagac
    64921 caattttttt catttgtcat tatgtactta tagcatctct ctacaagagt tgttttgttt
    64981 ggagttataa acttattatt gtttatagta gactttattt caattaaatg tttttcagca
    65041 tcctttttct tttcatagct tccataactt ttttgcttta ttttgccagt tgtttcttct
    65101 atgtactcca catatacatg aaatttttcc cctctttttc ttataaaagc tgatttgata
    65161 ttcatatatg tacactcctt tataagtatt attcatgttt aaaatatatt gaataactat
    65221 ggttttatta tatcaaatct aagttataaa ttctattcga tgatagagta gatgtataca
    65281 gaaatatata gacgaatctt taaaaaagta atatataggt aaaatatgta agaattatat
    65341 gttataatat ttttagcaag gaaaataaaa ttgaaaagcg aagggtgatt ttctcatata
    65401 aacgccaaat tccaatatag gaaggaggtg aatttatatg ttaattaact ttttattgag
    65461 tatagtagct ggtattgtat cagctttcat atatgacaaa ataaaaaatc acccaaacgc
    65521 caataagggt gatttaaaga aataactttt aaattctaat caattagaga aattcacctt
    65581 ttaaaaagta aattttatct ccttgctatt attatatcac aaattggtat agatattcaa
    65641 aaataatatt ttattaaagt ataattgttt gaatatattt aagctaattt attatttaat
    65701 tcttgtattt ttaatgtttg tctatattct tgtgcttctg ccaattttgt atattccaca
    65761 gtcttatcga aatttttctc aacttctgtt cttatttcat caagcgtaac tttgaagaat
    65821 tcctttctat tatttacctt gttaactctc ttattatcaa aagctttatg taaagctgat
    65881 tctaatttag gtgcatcttc actaaatata gtcgcatgta tatcatattt aaaaggaact
    65941 gatgcactac ttaattcatt tattctctca gttgggtcta atcttctagt tactcctatt
    66001 ttatatatat tttctccaaa actaccaata ttggagatta tgtatacatg tcctgctctt
    66061 gtattttgtt ctctatttaa tacatcatct aaatcttttg atagcaaagc tagtttttct
    66121 tctatagaat taattttttg ttcccattct aatttatcat tttcatcttc tgggatagtt
    66181 gattttagtc taagtaattc attcttgaat tgtaattctt ctttttttag ttttcttttc
    66241 tgattttcaa tttctttttg aacaagagct tcttctttca tttgttctct caaagctctt
    66301 tgttcttctt tttcctcttc tttcatttga aggtattcat aatataaata aagctcatct
    66361 attttcaaat taagatagtt ttcttttata gaaaccttat ttgaagtatt taatttattt
    66421 gtatcagtaa atgcttttcg tattcttttt tctatagaat caatattttt aaaactaact
    66481 tttgacatag caatgtcgca ctcactattg aagagtttaa gagctgtttt aatattttga
    66541 ttagtcatag ttctaccttt ttgaacactt ccatcaacag tccaagaatc agaatatttt
    66601 acacctgttt tatttcttat taatagtttt tgcatttctc ttatttcttt taatttttcc
    66661 atatattcat cagagttttc aaaatcatat tttggattat ataatccaaa tgattgaagc
    66721 agaacctcat cttcagtctg tattaattct tctgataatc tttttagctc ttctttttta
    66781 ttttttaaag ttgtgtttat atctctaagc tcttcccttt cattttttaa ttcttctaaa
    66841 tgagaattta aagtattaat attatcttta agtatatttt tatcttttat aagattatca
    66901 agtctttctt tttctatttt tagttcttcg ataagtttaa atttatcttg gttttctgaa
    66961 tcaaatttat caatttcaac tttctttttg tctaatagat tggataattc ttttaactta
    67021 ttttcatttg attcacataa atcttcaaag tgtttttctt tcattttaat ttcatcattt
    67081 aataaaatat atttattatt gattttttcg acatcagata atagtatact aatgctatct
    67141 ttatgttttt tcattatctt atttctttta aataagaata ttattgcaac tataaatgat
    67201 atagagtatg ttgaaaacgt agaaagaatt agtatggata tagtccataa agaataatac
    67261 cagggtatct tgaaattaag atttttgatt ttttcatcgt tgatgagtaa atcacttttt
    67321 aactgttcat tttcattgtt catatcaaaa ctcctttttt attttttgtt ctactttatg
    67381 ataataataa tttaagtata taaaatttta tgtgcaattt ccatatattt gcaaaaaaag
    67441 gtaaaaataa aacaaaatgg tagttattta aaagaattta gttggattat aaaggtatgt
    67501 taacagaaat tataaaaata atataacatt cttataaaag tatgttatca gtattttgaa
    67561 cataaggaaa agctacttaa aattagtttt aatcatttta agtagctctt ttattatttt
    67621 agatactcct ttgaatattt aatccaagca tcaataccat cgattaagtg aattaattcg
    67681 ccataaatat gttctgaacc aaattcattt ttcgtgtaca ttatccattt ttttctacct
    67741 tgcaatttaa ctttaccaat gaagttatct gaagtatcaa atacattaaa tgctccatct
    67801 gacattctat caaagacaaa attatttttt aattcatttt cgtacatttt aattttaagt
    67861 atattaaaaa agtagatttc attttcatta attttgaagt tgtaattttt attattaact
    67921 tcgaatggta ttgatttgtt atttttaaca atatccatat aatgtaaatt tttattatta
    67981 atactatcat ttgcgttata tgttatagta gagtgattgt atattacatc atttatacta
    68041 tttatagcat tattaatttc gatattatca attttttctc ctaccaaacg ttttactaat
    68101 atagaagttt tggagttatt tcctaacata tcacaaagta tataggatga tttaattatg
    68161 ttatctaaaa gtgtgtcttt taaatttata tctaattcct ttatggcttt gtaagactca
    68221 attttcatag gtgtaatttt atgattttcc cagtttatat taattcctct ttggatattt
    68281 ttagaggatt catatttaca aattaagtca tatgcttcct catatttatc aatataaatt
    68341 aattctaaac actggtcttc taggtcagta tttttagtaa cagatttacg cacatctttc
    68401 aacagttctt ttcctttatc tgttaaagca taatatttgt tggagctatt aaaaaaatta
    68461 gataaatctt tgcatgagat ttctttttct atcctttcta ttaattcagc ctttttacca
    68521 gttgatttaa tattattaga ttttaatatt tctttcaatt ccgaaactgt taacttagta
    68581 aggtcttcaa tgtaatttga aatcttaaga taatccatgt tgaaaaactt agaaattaaa
    68641 tatgaatagt taatattata ttcatatgtc caataggttg ctatataagg ctcgtatata
    68701 ttttctccaa ttatgtattt taggaaagat ttttctattg atgtaagtgg tttagtatct
    68761 gtttctaata tattgttttg tgggttaaac ttattaatac tattagtatg tatatcatat
    68821 aaagcatctg atttttctct tgcttgtttt tctaattcat ataaatttgt tgtatcatta
    68881 caacttacat tcagcacttt attaatttca ttttttactt ctatgttatg aggtgaatta
    68941 gaattacatt ctagtgaaag tgtagtacta tcattatagc tttgttttga ttcgttcgat
    69001 ttttcattgc aatcaacatt atttttgttt ttattttttc taaatagatt caaaatgctc
    69061 ataaaaaatt actccttttg tatttaatta atttctagtg tattctactt tgtgacaata
    69121 acaatttaag tatataaaac tttctgtata aatgctatgt atattaggaa aaaacataga
    69181 attaaatagt tttgaaaaga atactatatt tatatggtat aaaaagtaaa acgaagattt
    69241 taaaattaaa taataaaaaa tattatataa acaagaaaat acaaaaaatt ataattaaaa
    69301 agacgattaa taatacacag gaatggaaaa aataaccaag actctacaaa attcgatttt
    69361 tttgttacaa ttcccctttt tttattgcat atatttaaca aaagtattat tatgtaagta
    69421 agataattat ccaagaaaat tttgaataat ctaaaaatat ttttagaaat gaaaatacat
    69481 agttatattt tattaaaatt cattagggaa ttttgactaa aaataagaac gtaagttctg
    69541 atagtagggg gaaggcgtgt ggaaaaatca aaaaaactac taattgacac aggggataca
    69601 ttgaacaaat taagagaaaa taatagtgag aagttagaaa agtacataga attattaaaa
    69661 gaatattata aacaagaaaa taaaaaagaa agagaatagt tttcttctct ttcttatttt
    69721 ttctcattaa gttcttcttc aagaaatact tttgctattt tgaacatttt ttcttgacta
    69781 gaaggactta attcgctaat tagttttaaa gcttctttta tatcatcaga gaaatttaga
    69841 ttttcaatta gctctaactc actaagattg tctatatttt ttacattgac ctttccaagc
    69901 aaataatctg tagatacatt aaaaaaatct gataatttta aaattatgtc atgtggagga
    69961 aatctttctt ccttctcata aaaagaaatc attttaggag taagacctaa aaaagttgca
    70021 agttcttttt gagtaatatc tttttctttt cttaattcct ttattctata tccaatcatg
    70081 ttatttagcc acctttttaa tttatattga actattagtt cttaattatc aattatagct
    70141 catttttttt aaaaaagcta ttgacaatgt actaatagta ctgtactatt agtacataag
    70201 ataaagtaaa agaaggtgaa aaaatgaata aactaaaaga ttttagaata caaaaaggac
    70261 taagtagaaa tcagttagga gctgaattag gtcttacaag taggtatata gctttcttag
    70321 agaatggcga aagagtaccg tctctaaata cagctgtaaa aatagcagct tatttcaata
    70381 cttcaataga gtatattttt ttgaaaaaca actgtactaa aagtacattt aaagaaaaag
    70441 gagctgaaaa atatgaataa cttacaagta atagaaagaa ataacgaaag agttttaact
    70501 acacagcaac tagcagatgt atatgaaaca gatgcaagaa atataagcaa caattttaac
    70561 aataacaaag atagatttat tgaaggtaaa cattattttt tattgcaagg tgatgattta
    70621 aaaaatttta aaggtattca tacagaatat gaaaacctaa aatttacttc aaaaatgtat
    70681 ctttggactg aaagaggagc aaatagacac tgcaaaatat tggatactga caaagcttgg
    70741 gagcagtttg ataacttaga agaaacatat ttcaaagtta agcaacataa gccaacttgt
    70801 atagaagatg tattaataga aagtttaaaa gaaatgaaag atttaagact tcaagttaat
    70861 caagcaaata gcattgcttt agaagcaaag acagaggttg aaacaataaa agatgtagtt
    70921 tcattagact caaatagttg gagaacaaat acacatcaac taattgcaag aatagcaaaa
    70981 aaacaaggtg gttttgaaca tataaatatg cttagaacag aaagttatga attattaaat
    71041 aagagatttg gagttgacct acatagaaga ttaatcaata aaagaagaaa aatggcagaa
    71101 gaaggtgtat ctgaatctaa aagagagaaa gttaacaatt tagatgtaat acaagatgat
    71161 aagaagctaa tagaggggta tgtggctatt gtaaaagata tggctttaaa atatggaata
    71221 tcaagtgatt taagcaaaaa ttaggttaaa tcatgacagt accttgaaaa ctaaatacag
    71281 gatattcaat taatatgaga aggaggtttg aaaatgagtg tagcattaca attcatagac
    71341 acaaaagact tagtacaaga gttaatgcga agagatgata caacagacat catcaagatg
    71401 tttttagata gagaaggaat taaaagaatg gagttaatga ctatagaaga attttgtgag
    71461 tacttgaaaa tatctgatgt aacagctaga aacatggcaa gagaagccat gataacaaaa
    71521 gattttattg ctttaaaaat aggaagaaag tacatgattg atagaatatc atttgaagaa
    71581 tttattatga aaaatgcaat gaaagataaa gatgtaatga aaaaaagaaa gggggtgatt
    71641 tagttgaatg taagagtact gatagcttat gtacaattct gtaagcaata caataagaaa
    71701 gcaagttttg aaggtcttaa aaaatacaat aaggggatag tgatatgaaa atacttaaag
    71761 taaaatttcc tacaaaaatt cttaaagcag gtaaagcaat cagagtaaca tgtgcaagat
    71821 ttggttttga aagcgattgt ataataactc aatcacaaga atttgaatta acagtagttt
    71881 attttgataa agaattagat gatttagtac aatcgtctat aactatagat gatgcaataa
    71941 gctatgatta ttgtattgaa atgctaaatt aagggggatt aatcatgaaa agtttaatta
    72001 tagtgagaaa tgcagtagaa caacaactaa atagagctaa tttagaaata aataaaaacg
    72061 agcaacttta tacaaaactt agaaaaaaag aagaaagaaa tatatcagaa gaaattgaat
    72121 tgagtaatgc tttaagagaa aaaagtgtaa acgaaagatt aaaaatattt gctgagtcat
    72181 tactagaaat tatagataca caaattgaaa taaaagaata tgaagaaaat gaggattgca
    72241 agatatttca attaatttta gaagaacttg aaagagatag acctatagat gttcagatat
    72301 aagaaaagag ccattgccgt ggctcaattc aaataaatat atcaaaattt aattaagcta
    72361 attatagcac aaacggaggg aaattatgag tactttatat gaattaacta cagatttatt
    72421 agaaatagaa gaaggtttaa cagaaacaac aggaaatgaa actgaaaaac tagaggaaat
    72481 aaaagaaata ataaaacaag agatacaaaa taaaaacact aggatagttt cagtaatatt
    72541 aaacattgac agtgatataa actctataga ttcagagatt aagagaatgc aagagttaaa
    72601 gaagattaag aagaatgctc ttgacagatt aaaaagcaat ataaaggaat gtatggaact
    72661 acttggtact aaaaaagtag aaacattttt aggaaatata agtataagaa agtcagcagg
    72721 tagcttagtc atagaagatg aagaaaagat acctgctata tataaaacag tagagcaagt
    72781 tgtaaaagta gataaaaata gtataaaaga ctttattaaa aaaggtcatg aggtcgaagg
    72841 ttgcaggatt gaatatggaa ctacactaac aattccaaaa gctaaaaaag agtaggtgag
    72901 gaccatggaa attaataata tttacattaa attgatgaat gtaagagtta aatttagtaa
    72961 gttgaattta aagaaaagtg gagaaaataa gttcgctaac ttcaagtatt ttgagttagc
    73021 agactttcta ccacaagcaa ctggattact tgaagaagct aagctatgcc ctatagtgac
    73081 ctttacaaat gaatatgcaa ctctaacatt aattaatgga gaaaacccat cagagcagat
    73141 tgtattcact tctccaatga gagacttgca acttaaaggt tctaatgaat tacaagcact
    73201 aggaggtata gaaacctatc aaactagata tttatatatt cagttactta atataactga
    73261 aagtgacact tttgacgcaa c
//
LOCUS       DAYAOY010000006        71682 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYAOY010000006 DAYAOY010000000
VERSION     DAYAOY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.006"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(130..324)
                     /locus_tag="ACGI6B_000459"
     CDS             complement(130..324)
                     /locus_tag="ACGI6B_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749928.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI6B_000460"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI6B_000460"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFG9749929.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI6B_000461"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI6B_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFG9749930.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI6B_000462"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI6B_000462"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFG9749931.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="ACGI6B_000463"
     CDS             complement(3870..4640)
                     /locus_tag="ACGI6B_000463"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HFG9749932.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="ACGI6B_000464"
     CDS             complement(4659..5249)
                     /locus_tag="ACGI6B_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9749933.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="ACGI6B_000465"
     CDS             complement(5432..6400)
                     /locus_tag="ACGI6B_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HFG9749934.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="ACGI6B_000466"
     CDS             complement(6393..7370)
                     /locus_tag="ACGI6B_000466"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFG9749935.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI6B_000467"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI6B_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFG9749936.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI6B_000468"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI6B_000468"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFG9749937.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="ACGI6B_000469"
     CDS             complement(11260..11688)
                     /locus_tag="ACGI6B_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFG9749938.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI6B_000470"
     CDS             complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI6B_000470"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFG9749939.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="ACGI6B_000471"
     CDS             complement(12713..13198)
                     /locus_tag="ACGI6B_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFG9749940.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI6B_000472"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI6B_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HFG9749941.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="ACGI6B_000473"
     CDS             complement(14053..14733)
                     /locus_tag="ACGI6B_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9749942.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI6B_000474"
     CDS             complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI6B_000474"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFG9749943.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="ACGI6B_000475"
     CDS             complement(15725..17275)
                     /locus_tag="ACGI6B_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HFG9749944.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="ACGI6B_000476"
     CDS             complement(17369..17635)
                     /locus_tag="ACGI6B_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFG9749945.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI6B_000477"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI6B_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HFG9749946.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="ACGI6B_000478"
     CDS             complement(18727..19665)
                     /locus_tag="ACGI6B_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFG9749947.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="ACGI6B_000479"
     CDS             complement(19775..19936)
                     /locus_tag="ACGI6B_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749948.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="ACGI6B_000480"
     CDS             complement(19946..20443)
                     /locus_tag="ACGI6B_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFG9749949.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="ACGI6B_000481"
     CDS             complement(20558..21268)
                     /locus_tag="ACGI6B_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HFG9749950.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="ACGI6B_000482"
     CDS             complement(21444..21974)
                     /locus_tag="ACGI6B_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749951.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="ACGI6B_000483"
     CDS             complement(21991..22221)
                     /locus_tag="ACGI6B_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749952.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="ACGI6B_000484"
     CDS             complement(22236..23630)
                     /locus_tag="ACGI6B_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFG9749953.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="ACGI6B_000485"
     CDS             complement(23756..25009)
                     /locus_tag="ACGI6B_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFG9749954.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="ACGI6B_000486"
     CDS             complement(25060..26214)
                     /locus_tag="ACGI6B_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9749955.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="ACGI6B_000487"
     CDS             complement(26774..27343)
                     /locus_tag="ACGI6B_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9749956.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="ACGI6B_000488"
     CDS             27546..27773
                     /locus_tag="ACGI6B_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9749957.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="ACGI6B_000489"
     CDS             complement(27992..28735)
                     /locus_tag="ACGI6B_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9749958.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="ACGI6B_000490"
     CDS             28903..29775
                     /locus_tag="ACGI6B_000490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9749959.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="ACGI6B_000491"
     CDS             29775..30440
                     /locus_tag="ACGI6B_000491"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9749960.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="ACGI6B_000492"
     CDS             30461..31318
                     /locus_tag="ACGI6B_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9749961.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..34020)
                     /locus_tag="ACGI6B_000493"
     CDS             complement(31474..34020)
                     /locus_tag="ACGI6B_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9749962.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34020..34706)
                     /locus_tag="ACGI6B_000494"
     CDS             complement(34020..34706)
                     /locus_tag="ACGI6B_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9749963.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="ACGI6B_000495"
     CDS             complement(34789..35778)
                     /locus_tag="ACGI6B_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9749964.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="ACGI6B_000496"
     CDS             complement(35765..36451)
                     /locus_tag="ACGI6B_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9749965.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36448..36570)
                     /locus_tag="ACGI6B_000497"
     CDS             complement(36448..36570)
                     /locus_tag="ACGI6B_000497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749966.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(36951..40157)
                     /gene="carB"
                     /locus_tag="ACGI6B_000498"
     CDS             complement(36951..40157)
                     /gene="carB"
                     /locus_tag="ACGI6B_000498"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFG9749967.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40350..41399)
                     /gene="carA"
                     /locus_tag="ACGI6B_000499"
     CDS             complement(40350..41399)
                     /gene="carA"
                     /locus_tag="ACGI6B_000499"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFG9749968.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41656..44862)
                     /gene="carB"
                     /locus_tag="ACGI6B_000500"
     CDS             complement(41656..44862)
                     /gene="carB"
                     /locus_tag="ACGI6B_000500"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFG9749969.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44915..45958)
                     /gene="carA"
                     /locus_tag="ACGI6B_000501"
     CDS             complement(44915..45958)
                     /gene="carA"
                     /locus_tag="ACGI6B_000501"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFG9749970.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="ACGI6B_000502"
     CDS             complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="ACGI6B_000502"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFG9749971.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47076..47711)
                     /locus_tag="ACGI6B_000503"
     CDS             complement(47076..47711)
                     /locus_tag="ACGI6B_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HFG9749972.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47735..48649)
                     /locus_tag="ACGI6B_000504"
     CDS             complement(47735..48649)
                     /locus_tag="ACGI6B_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9749973.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49250..50479
                     /locus_tag="ACGI6B_000505"
     CDS             49250..50479
                     /locus_tag="ACGI6B_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9749974.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(50684..53065)
                     /locus_tag="ACGI6B_000506"
     CDS             complement(50684..53065)
                     /locus_tag="ACGI6B_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HFG9749975.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(53037..53762)
                     /locus_tag="ACGI6B_000507"
     CDS             complement(53037..53762)
                     /locus_tag="ACGI6B_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HFG9749976.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(53752..54207)
                     /locus_tag="ACGI6B_000508"
     CDS             complement(53752..54207)
                     /locus_tag="ACGI6B_000508"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFG9749977.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(54489..55670)
                     /locus_tag="ACGI6B_000509"
     CDS             complement(54489..55670)
                     /locus_tag="ACGI6B_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9749978.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(55660..56352)
                     /locus_tag="ACGI6B_000510"
     CDS             complement(55660..56352)
                     /locus_tag="ACGI6B_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9749979.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(56486..57202)
                     /locus_tag="ACGI6B_000511"
     CDS             complement(56486..57202)
                     /locus_tag="ACGI6B_000511"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9749980.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            57327..58061
                     /locus_tag="ACGI6B_000512"
     CDS             57327..58061
                     /locus_tag="ACGI6B_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFG9749981.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(58346..59101)
                     /locus_tag="ACGI6B_000513"
     CDS             complement(58346..59101)
                     /locus_tag="ACGI6B_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9749982.1"
                     /translation="MKKYNISENGALIKNDSIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(59223..60161)
                     /locus_tag="ACGI6B_000514"
     CDS             complement(59223..60161)
                     /locus_tag="ACGI6B_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFG9749983.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(60264..60914)
                     /locus_tag="ACGI6B_000515"
     CDS             complement(60264..60914)
                     /locus_tag="ACGI6B_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9749984.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            complement(61063..61251)
                     /locus_tag="ACGI6B_000516"
     CDS             complement(61063..61251)
                     /locus_tag="ACGI6B_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFG9749985.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(61358..62542)
                     /locus_tag="ACGI6B_000517"
     CDS             complement(61358..62542)
                     /locus_tag="ACGI6B_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9749986.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(62684..63601)
                     /locus_tag="ACGI6B_000518"
     CDS             complement(62684..63601)
                     /locus_tag="ACGI6B_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HFG9749987.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(63623..64351)
                     /locus_tag="ACGI6B_000519"
     CDS             complement(63623..64351)
                     /locus_tag="ACGI6B_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9749988.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(64547..64747)
                     /locus_tag="ACGI6B_000520"
     CDS             complement(64547..64747)
                     /locus_tag="ACGI6B_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749989.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(64872..65252)
                     /locus_tag="ACGI6B_000521"
     CDS             complement(64872..65252)
                     /locus_tag="ACGI6B_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9749990.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(65525..66883)
                     /locus_tag="ACGI6B_000522"
     CDS             complement(65525..66883)
                     /locus_tag="ACGI6B_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9749991.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            67331..67783
                     /locus_tag="ACGI6B_000523"
     CDS             67331..67783
                     /locus_tag="ACGI6B_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HFG9749992.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(67940..68557)
                     /locus_tag="ACGI6B_000524"
     CDS             complement(67940..68557)
                     /locus_tag="ACGI6B_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HFG9749993.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(68980..69792)
                     /locus_tag="ACGI6B_000525"
     CDS             complement(68980..69792)
                     /locus_tag="ACGI6B_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9749994.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(69868..71286)
                     /locus_tag="ACGI6B_000526"
     CDS             complement(69868..71286)
                     /locus_tag="ACGI6B_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFG9749995.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT        22663 a        13138 c         7738 g        28143 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgttgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttgc tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttc ttataactat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaatta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaagcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtacctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag ttaatacatt cctaacaata
     6661 tctactctgg ataacatata tccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggggct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctataatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgct ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcacc atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa gccttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcagtttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaataata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatatttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttccca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcatt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggca caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaagaaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgccata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccga ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gtattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaaataaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtatcc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gagttcatta atcttctact agcttggtct cttgtcaatc cactgatacc
    25261 atccttaagt ttaaatgaaa ctacgcctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgtgatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tctccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatgcct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcaat acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcattt tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agatatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagagaca cctatgaatc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttgtg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc ccctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttatc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gttcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agtaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttccac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactcccc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 catagaactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 cagtgcgtaa ttttgctctc taatgcgtaa cgtcatttta gttatttcac tttgtagaat
    35521 agaaagctca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc agttgctgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggtt cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc ttcgctgttc cttctgtagc aacaaaagta
    37261 tatcctaatt ctttcatatc ttttgctatc tctataaact catctttatc atgattattt
    37321 acagtagcaa gtactactcc tctttcatca gacatatgtc tacctgctgc caagaaccct
    37381 ttatataagg cttcttctaa attttctcct acacctagga cttctcctgt agacttcatt
    37441 tcaggtccta gacttacttc aactttagat aatttagaca ttgagaatac tggtactttt
    37501 actgatacta attttggctc tttgtaaact cctgtaccat atcctaaatc ctttaattta
    37561 gctcctaaca tacacttagt agccaagtca actattggta ctccacttac tttacttata
    37621 tatggaactg ttctactagc tctagggtta acctcaatta tatataattc attttgaaat
    37681 tctataaact gtatattaac cataccaagc acattaaggt ctaaagctat ttttttagtg
    37741 tattctacta tcttttcttt tatttcatca gatatatttt gacttggata cattgttgta
    37801 ctatctccag agtgaactcc agctctttct aaatgctcca ttataccagg tatcaatata
    37861 tcttctttat cacatatagc atctacttct atttctctac cagttagata tttatctata
    37921 agtactggat ttttatggtc tctatcaaat gcatcatcca aatactgaat tagtttttct
    37981 tcgttataag ttatttccat accttgacca ccaagtacat aagatggtct aactaatact
    38041 ggataaccta actccttagc aatttcaaca ccttcattag ttgtccatac accttttcct
    38101 tttggtctat ttatatctag tctctctagt aaatcatcaa atttttctct atcttctgct
    38161 gcatctatat ctctaaagtc tgttcccagt ataggtatat ttttttcatg taaaaactta
    38221 gccaatttta tagctgtttg tccaccaaac tgtagtatta caccttctgg tttttctttt
    38281 tctattatat ttaaaacttc ttcttctgtt aatggttcaa aatacaactt atcagatgta
    38341 tcaaaatccg tacttacagt ttcagggtta ttgtttacaa ttatagtttc tattcccatt
    38401 tttcttaaag attttacaca gtgaactgaa caatagtcaa attctattcc ttgacctatt
    38461 cttattggac cagaacccaa tactacaact tttctcttat cactaactac tacctcatca
    38521 tattgttcat aagttgaata gtaataaggt gatagagctt caaattctcc tccacatgta
    38581 tcaaccattt tataaactgg ttgtatatta tataaacttc tcaattcata taatttttct
    38641 gggcttatct tcattaagtc agatatacct ttatcagaaa atcccttttt ctttaattca
    38701 tgtaaatatt ctttatctaa gtcttctatt tttagaactt ttaacttttc ttcttgttct
    38761 actatccatt taaatttatt tataaaccac ttatcaacac cagttatttc tgatatcatt
    38821 tctactttat agcctcttct tatcatttca gctaagtcaa atagtctctc atcatcaggt
    38881 actactactc tagcctttaa ttcttctaat gttctacctt ctgatggagc atgaaccaat
    38941 gaatattttc ctgtttcaag tgatcttata cctttaagaa tagcagcctc aaagttactt
    39001 cctatactca tgatttcacc agttgccatc atcttagttc ctaattttct attggcatgt
    39061 ctaaatttat caaatggcca ctttggtatt tttactacca cataatctaa tgttggctca
    39121 aaacaagcat aagttttttt agttactgca ttttctatct catccaaagt ataacctaaa
    39181 gctatcttag ctgctacttt tgctattggg tatcctgtag cttttgatgc tagggctgat
    39241 gacctactaa ctcttgggtt tatttctatt atcgcatatt ctaaactatg tgggtttaaa
    39301 gctatttgaa cgttacatcc accttgaacc tctattgcat ttattatgtc tattgatgcc
    39361 tttctaagta gttgatactc ttcatcactt agagtttgac ttggagcaac aactatactg
    39421 tctcccgtat gaactccaac tgggtctaca ttttccatat tacatacagt tatacagttt
    39481 ccatttccat ctctcattac ttcatattct atttctttcc aaccttttat actcttttca
    39541 agaagaactt gtccaacagg acttagctgt agtccatgtg aaagtatttc tcttaattct
    39601 tcctcagtat cagctatacc tccaccagtt ccacctagcg tatatgctgg tcttacaact
    39661 actggatatc ctatcttatt ggcaaactca agacctgctt ctaaatttgt aactatatca
    39721 ctaactatta ctggttgatt tatttctttc attacttctc taaataaatc tctatcttcg
    39781 ccctttttta tagattctat agatgttcct ataactttta cattatattt atctaatata
    39841 ccagcatcat gtaattctac agctaagttt aatcctgtct gaccacccat acctgcaagt
    39901 aaactgtctg gtctttcttt ttctattatt ttttctataa attctattgt taatggttct
    39961 atatatattt tatctgcaat ttctttatca gtcattatag tagctgggtt actatttact
    40021 aatacaactt caattccctc ttcttttaat gcttggcagg cctgtgttcc tgaatagtca
    40081 aactctgcag cttgacctat tattattggc cctgacccta atactaaagt tttctttata
    40141 ctatctaatt taggcataac tctttctcct tatctaaaca attttcacag ttcttatctt
    40201 catcatctat acaatcaact ttaattactt tattttatct taagttcaaa tttactcatc
    40261 aatacatcta tttaatacat ctatgttaat taaaccaaac tttttgattt aagttattct
    40321 tgttttttag ttagctattc taatgttgtt tattttacta attctaaaaa tttatcaaaa
    40381 atatagtcat tatcttttgg tcctgggcaa gcttcaggat ggtattgaac actgtataca
    40441 tctagcttct catgtcgcat tccttcaaca gtattatcat ttaaatttat atgtgtcact
    40501 ttcatttgtt caggaacttc tgaaacataa taaccatgat tttgagaagt tataaatact
    40561 tttccttctt ctaaatcttt aactggatga tttccacctc tatgcccaaa tttaagttta
    40621 gcagtttttc ctccaagagc taaagctaaa agttgatgtc ctaagcatat tccaactata
    40681 ggttttttac caataagagc ttttatattt tctataacat cttctaagtc ttctgggtct
    40741 ccaggtccat ttgacaagaa tattaagtca gggtttatac tcaatacatc ttctggagat
    40801 gttgtagctg gaaatattgt tatatcacat ccacgagcta taaatgatct taatatattt
    40861 ctttttactc caaaatccat aacagccact tttggaccat ttccttttat atgctctact
    40921 tcttttcttg taacagttct aactgcttct gtatttgaaa acttatccaa tttatgttta
    40981 acatcttcta atttactgtc ttctaatgta attatacctt tcattgttcc attatttcta
    41041 agtatcttag tcaaagctct tgtatctatt ccttcaagtc cgataacttt attttgcttt
    41101 aaatattggt ctatatcaat ttcacatcta aaattacttg gagaatcact tttctccctt
    41161 actatgaatc cctttacttg aactttttca gactctgctg atgataaatt tataccataa
    41221 tttcctacaa gaggataagt catagtaact atttgaccat agtaagaagg gtctgttaaa
    41281 acttcaccat atcctatcat cgaagtatta aatactactt ctcctacact ttcttctaaa
    41341 tatccaaatg cctttcctat aaaaactgtt ccatcttcta atattaacct tgctttcata
    41401 ctacagtacc tcctataatt ctctttagac aaactaaaga acattaacta aatataagct
    41461 tttaaatatc tatatctatt tttcactttt acattctagt tttatactta tttacattta
    41521 ttttttatct ttttctatta tactacaaat ttcttaaaaa aaagaagtta tctcaaaagt
    41581 aaaaactcct agaaaaaata acctcttttt tttatttaat aattaaattc ttaaactata
    41641 caatcaactt aatttctatt cagctatatt gtaaactttt aactcatctt tatttaaatg
    41701 tttcttctta acttctacta aagcttttag tgtatccaaa gatgtcatta tttcagtaga
    41761 aaattcaata gctgttcttc ttatcttaaa tccatctctt gtagagtcat ttcctttagt
    41821 tggtgtattt attactatat caacttgttt atttcttata gcatctaata tgtttggtct
    41881 agattcttct actctattta ctatatcagc ctctattcca ttttctctta agctattagc
    41941 tgttccttct gtagcaacaa aagtatatcc taattctttc atatcttttg ctatctctat
    42001 aaactcatct ttatcatgat tatttacagt agcaagtact actcctcttt catcagacat
    42061 atgtctacct gctgccaaga accctttata taaggcttct tctaaatttt ctcctacacc
    42121 tagaacttct cctgtagact tcatttcagg tcctagactt acttcaactt tagataattt
    42181 agacattgag aatactggta cttttactga tactaatttt ggctctttgt aaactcctgt
    42241 accatatcct aaatccttta atttagcccc taacatacac ttagtagcca agtcaactat
    42301 tggtactcca cttactttac ttatatacgg aactgttcta ctagctctag ggttaacctc
    42361 aattatatat aattcatttt gaaattctat aaactgtata ttaaccatac caagcacatt
    42421 aaggtctaaa gctatttttt tagtgtattc tactatcttt tcttttattt catcagatat
    42481 attttgactt ggatacattg ttgtactatc tccagagtga actccagctc tttctaaatg
    42541 ttccattata ccaggtatca atatatcttc cttatcacat atagcatcta cttctatttc
    42601 tctaccagtt agatatttat ctataagtac tggattttta tggtctctat caaatgcatc
    42661 ttgtaggtat tgagataatt tttcttcgtt ataagttatt tccatacctt gaccaccaag
    42721 tacataagat ggtctaacta atactggata acctaactca ttagcaactt caataccttc
    42781 gttaagtgac catacaccct ttccttttgg tctatttata tctagtctct ctagtaaatc
    42841 atcaaatttt tctctatctt ctgctgcatc tatatcatca aaatcagttc ccaatatagg
    42901 tatatttttt tcatgtaaaa atttagccaa ttttatagct gtttgtccac caaactgtag
    42961 tattacacct tctggctttt ctttttctat tatacttaaa acttcttctt ctgttaatgg
    43021 ttcaaaatac agcttatctg atgtatcaaa gtctgtactt acagtttcag ggttattgtt
    43081 tatgattata gtttctatgt ccatttttct taaagatttt acacagtgaa ctgaacaata
    43141 gtcaaattct ataccttgac ctattcttat tggaccagaa cctaatacta tcaccttttt
    43201 cttatcacta acaactacct catcatattg ctcataagtt gaataatagt aaggagatat
    43261 agcatctatc tcacctgcac aagtatcaac cattttatat gctggtttta tattgtatag
    43321 acttctcaat tcatataatt tttctggact tatcttcatt aggtcagata tacctttatc
    43381 agagaatccc tttttcttta attcaagaag atagtcttta gttaaatctt caattttcat
    43441 aacttttaat ttttcttctt gctctactat ccatttaaat ttatttataa accatttatc
    43501 tatacctgta atttgttcta tcatttctac tttatagcct cttcttatca tttcagctaa
    43561 atcaaatagt ctctcatcat caggtactac aactcttttc tttaattcct caatgcttct
    43621 ctcctcagat ggtgtgtgaa ctaatgaata ttttcctgtt tcaagtgatc ttatcccttt
    43681 aagaatagca gcctcaaagt tacttcctat actcataatt tcaccagttg ccatcatctt
    43741 agttcctaat tttctatttg cttttttaaa tttatcaaaa ggccattttg gtatttttgc
    43801 caccacataa tctatagttg gttcaaaaca agcataagtt tttttagtta ctgcattttc
    43861 tatctcatct aaagtataac ctaaagctat ttttgacgaa actttagcta ttggatatcc
    43921 tgtagctttt gatgctaaag ctgatgacct actaactctt ggatttatct ctataactgc
    43981 atattcaaaa ctatgtggat taagtgcaaa ttgaacgtta caaccacctt gaacttctac
    44041 tgcatttaat atatctaaag atgctttttt aagcattgca cattctttgt tacttaaagt
    44101 ttgagttgga gcaacaacta tactgtctcc tgtatgaact ccgactgggt ctatattttc
    44161 catgttacat acaactatac aatttccctt tgaatctctt attacctcat attctatttc
    44221 tttccaacct tttatactct tttcaattaa tacttgtcca acaggactta attgtagacc
    44281 atgtgaaagt atttctctta attcttcctc attatcagct atacccccac cagttccacc
    44341 tagtgtgtat gctggtctta caactactgg ataaccaatt gtaagtgcat aatcaagacc
    44401 tgcttgtaaa tctgtaacta tatcactaac tattacaggc tgatttattt ctttcattac
    44461 ttctctaaat atatctctat cttcaccttt tttgatagat tctactgaag tacctattat
    44521 ctttactcca tatttatcta gtatgccttt ttcataaagt tcaactgcta ggtttagccc
    44581 tgtttgtcca cccatacctg caagtaaact gtctggtctt tctttttcta ttattttttc
    44641 tataaattct attgttaatg gttctatata tattttatct gccacttctt tatcagtcat
    44701 tatagtggct ggattactat ttattaatac aacttcaatt ccttcctctt tcaaagactg
    44761 gcaagcttgt gttcctgaat agtcaaactc tgcagcttga cctattatta ttggtcctga
    44821 ccctaatact aaagttttct ttatactatc taatttaggc ataaattttt ctccttatct
    44881 aaatggtttt tactatctgt aaataatact aaatctatat taatgctaag aatttattaa
    44941 atatacaatc taaatcttct tttgttgtgt attctggaat gtattgaaca ctatatattg
    45001 gtaattcctt atgtctcatt ccttctatag tattgtcatt taaatttatg tgagttactt
    45061 ctgtattttc tggcatttct gaaacattat atccatgatt ttgagaagtt ataaatacct
    45121 ttccagtttc taaacattta actgggtgat tacctcctct atgtccaaat acaagctttg
    45181 aagtctttcc tcctaatgcc aatgctaaaa tttgatgtcc taaacctatt cctgtcaatg
    45241 gctttttgcc tattaatttt tttatatttt caactatatc atttatatcc tctggatttc
    45301 ctgggccatt agataataag attaagtcag gatttatatt taaaatttct tctactgtag
    45361 tcattgctgg tactatagtt atatcacagt tacaagcctt aagagactct agtacactat
    45421 ttttaacacc aaagtctata actacaactt tagctccagt accttttata ttttcaattt
    45481 cttttcttgt aacatttttt actgaatctt tgttacaatg ctcttctaat ttagactgaa
    45541 catcttgtaa tgatacattt tctaaagtta taattcctct cttagttcca ttgtttctaa
    45601 taattttagt taatgctctt gtatctattc cttcaagtcc tattacctta ttttgcttta
    45661 gatatgtgtc tatatccatt tcacatctaa aattatttgg gttatcactt ttttctctta
    45721 caataagtcc ttttacttgc accttatttg attcaacatc ttctaaattt attccatagt
    45781 ttcctataag tggataagtc atagtaacta tttgtccata ataagttgca tctgtcaata
    45841 gttctccata cccagccatt gaagtattaa atacaacttc tccaatagct tcctttagat
    45901 atccaaatgc ttttccttca aatactgttc catcttctaa aattaatttc cccttcatgt
    45961 aagctacctc ccaaatattt tatctaagca ttttctattt ctcttacaat attaatagct
    46021 gattctcttg gattttctgc ttttgttata ggtcttccaa caactaaata atgagcacct
    46081 ttttttattg catctgatgg agttacaact cttttttggt ctccaacctc tgctgattta
    46141 ggtcttattc ctgggcaaac tgttataaaa tcagttccac atgcttctac tattttatct
    46201 acttcttgcg ctgaacaaac aaccccatct attcctgatt cttttgccag tttagctctc
    46261 tttatagcta actcttctgt tgtcatatta catcctatgt cctgtaaatc ttggttgctt
    46321 aatgaagtta aaactgttac tccaactact aatggctttt ctatatttaa tctttctgct
    46381 tcttccctag caattagtgc acattgtctc attccttcca tatctgatac atgtattgtc
    46441 attaaccaaa cattatctct cactgcagct ttcactgcac ttttcatagt atttggtata
    46501 tcatggaatt taaggtctaa aaatattttt ttcccttttt ctttaagata atctattgtt
    46561 ttaccttttg tggcaacgta ttgctctagc cctaccttaa atatgtctac actatcttct
    46621 aatctgtcta ttagttcttt agctttatca aattcgtcag tatcaattgc aactatcaat
    46681 ttttctttac catttatcat ttttacatct cctttttaat aaagcctaaa taatcataaa
    46741 aaaatcctca caccaaaaga ggcataagga ttttatctat acataactct attcattagc
    46801 tatgagatat ctttcccagt ctctctggac tagtttaaaa gactttatta tatttaaatt
    46861 tttattttat actcatcaaa aacctctaga agatttacta gaggttatat actaacatta
    46921 tctataaccc tttagtctct ctggactact ttaaggaatt tagctgtaat atttttcttt
    46981 atttatataa ctttatgtat ctataggata atttaaagtt ttatcttttt tataattaaa
    47041 ccactaattg ctttttatgt caataagtct ttgaattatt cttcatcttt atcaaacaat
    47101 tttttctcaa aatatgattc tgcattatat attgcagcaa ctggattatg cccagttact
    47161 ctatctttga caactaaagt tgttatagga gcttccatat atttcatggc taaagtatcg
    47221 tgaccaacac ataatccaag aattacatta aggtcaacct tagcttcatt taaaaataaa
    47281 gcttgtccta ttggattgca cattacctca tctgaacact ttgatattgt ttgagatgct
    47341 tctagtccaa ttgtactctt aggcattgcc ccatttttac atataacaga aaatacttca
    47401 aacccatgat gttcaaatat tttttgcact gtttgcattt ctctttttag tccaatacaa
    47461 aatgctatgc caatcttttt ataaccacac tttttagcaa actctataat ctcttttagt
    47521 ctagtatatt gacaataccc ctctgcttca actaatccag cattataagc tatttttcta
    47581 ttctcttctt cttcataaag ttgctttgct ttttgttgta gttcaatttc tctagttgga
    47641 catatcttca ttaatttatc catatttcca ttagaacagc ctctaagatt acattttcca
    47701 cacttataca tatccaatat ccccttccaa aattttattc atcttgcgat tcttcttttt
    47761 ctagagttac attttcttct tgggtttctt cttctggttt cacatgctcg actacagtta
    47821 taaaatatga ttttcctatt ttattcctta taggaagttt ttcatatgaa acatctattt
    47881 tattttttat acaagctata tcaaaatgtc ttaaggctac acctaagcta accttattca
    47941 tatcttcaca gtgtttctta ggtctctttt caaataaatg aagacctcgt tcatccttat
    48001 ggaaatacca cggttgagaa ttttttacag atggagctct tcttgtcaat tctaagatat
    48061 ctttccactt tctattccta tttatcttat ttatcatatc ttttactggc tttctatcag
    48121 gtctagcata tctcgtccta aacaactctt cattttttgc tggatatcca aatgctatta
    48181 ttatataagg attttctatt ttgttttcat catcttcttc tgtttttatt ccagctagat
    48241 aatctaaatc atcttcctca tcttcttcaa aattcttatt cccaatatct acaaattcca
    48301 agatatcttc tctctttaag tttccttcca accagcaagt agctattccc aatgttgtaa
    48361 ggcgcaaaac tacaccttct gtagcaaatc ctatattctg taaatagtcc tcacctttgt
    48421 ctgatgtaac tactatataa tgaggtgcct ttactttaca ttccttcccc atcaaaaaat
    48481 gtattatatg acctctatca acaacatgtg ctttaatatt taaatcttca tttaagtatt
    48541 ctaaattttt acaacttctt tttatttctt ccatcaatgg cttttttatc tttttactgg
    48601 aaaaatttct tacagatttt ctataaaaaa tggcttcata aagctccatt tatgccctcc
    48661 taactgaaat cttctgacgt ataaactcta taaatttata taaatacttt atatttttta
    48721 tagttttatt tcctttttcc tcctgcccta ttttgaaatt cataagaata tctactctag
    48781 tttaatatag cattcaaaag gcttaaattc taccttatca atgtctatac attagcataa
    48841 tttttttatt tgattttcta tgctaattta aataacaaca ctagctatta cctttttaat
    48901 taagctttgc cagaaaaatt ttttttcatt tataaatatc tatctcatta ttatattata
    48961 agtttttttc ggtattcttg tatagctttt cactaaaaaa gtatttttat ttctacttta
    49021 atattattat caacaaaaaa catactactt aaataagttt gttatcctca taatttgttt
    49081 taattaaaat atttttttta tttctcaatc tataaagtat aaatattttg aatatgaccc
    49141 aaaatctaag taaagttata atattttata agttgttcaa tattaaaaac ataagtaatg
    49201 ttataatact ttataagtta ttcaatatta aaaatatagg aggataaata tgaatttaaa
    49261 tagaaattta gaaaaatacg ctgaattagc aataaaagtt ggagttaaca tacagccagg
    49321 tcaaattcta cttataaagt caccaataga atgtgctgat tttgcaagaa atgcacttaa
    49381 agaagcatat aaatgtggtg ctaaaaatgt ttatatagag tggtctgatg aagaaagtac
    49441 tcttattaaa tacttatatg ctccagatga agcatttcat gagttcccca aatggacagc
    49501 agaacaatat gtagatatag caaaagaagg tggggcattt ttatcagtct atgcccaaaa
    49561 tccagattta ttaaaagatg ttgaccctga aagagttgca aattttcaga aggcatctgg
    49621 aaaagcttta aaagaatggc gttcttacac tctaactgat aaatgtaaat ggagtatagt
    49681 ctctgtgcca acaaaagatt gggcaaaaaa agtgtttcca aatttatcag aagataaagc
    49741 aatagaaaaa ctgtgggatg ctatatttaa atgttcaaga gtagatggtc aggaccctat
    49801 aaaagcatgg gaagaacata atgaaaatct taaatctaaa atggactttc taaataaaaa
    49861 taactttaaa actttaaaat ataaatcctc aaaaactgat ttgactctag acttgccaaa
    49921 gggtcatgtt tggctaagtg gagcatccaa agaccctaat ggaataagtt ttaatccgaa
    49981 tattccaact gaagagattt ttggaatgcc acataagttt aaagtaaatg gaactgttta
    50041 tagtacaaaa cctcttgttt atggtggaaa cataattgac aatttcttct taacatttaa
    50101 agatggtaaa ataatagatt tctctgctga aaagggacta gatacactag aaaaacttat
    50161 tgaaacggat gaaggctctc attatttagg agaagttgct cttgttccat acaactcacc
    50221 aatatccaat acggatataa ttttttataa tactctatat gatgaaaatg cttcttgcca
    50281 ctttgcaatt ggttctgctt ataagacttg tctggaaggt ggaaacaatt taaaagatga
    50341 agaattagat gaacatggtg taaatgacag tttaacacat gtagatttta tgattggttc
    50401 tgctgatatg gatattattg gtgagactta tgatggaaag caaatacaga tttttaaaaa
    50461 tggtaattgg gctttttaac atatgatttt aactaaaaag ggctgatgga agttatacca
    50521 gacttcccag ccctttttaa tgttgatgat aaatattttt tatattaatt aatctcatta
    50581 ttatagatta attcctattg ttttaaacat aagtatctaa aacttatatt tatattatat
    50641 ataaaatatc ctatttagta aaacctttat tttaatttat atgctaaaaa tttaactttg
    50701 ctatttcaac agcagacttt gcatcctttg aataataatc agcacccatt tcttcagcgt
    50761 attcttctgt caaaactgca cctcctacaa atacctttgc atttatctca ttatctctca
    50821 gtgcctgaat tgtatctttc atactttgaa cagtcgtagt cataagtgca cttaatccta
    50881 ctaattttat atttctcttt ttagcaactt ccactacttc ttcaataggg acatctttac
    50941 ctaaatctat aacttcatat ccataatttt ctagcattat ttttactata tttttcccaa
    51001 tatcatggat atcacctttt actgtagcaa ctattatttt tcctttggat acattattgc
    51061 tacttttact taataaagtt tcctttattg tatttaatga aacttttacc gtttctgctg
    51121 attgaatcaa ttgtggtaaa aatatatctc cactatcata tctttttcct actttatcaa
    51181 gagcaggaat taaaatctca tctaatatat aattttcatc cttctctttt aaaagattca
    51241 gtgttacatt ttttgcttca tcttttaatc ctctttcaac taaaatatcc aagggcaaat
    51301 ctttatcaat ctttgtagat gaatccgatt tttttgaatt atttatattg ctatattgat
    51361 ttatatagtt tatacatcct ttatctgtgt tatttaatat tttaaaagaa ttgatagctt
    51421 ccatcatact atattcattt ggatttataa ttgctaaatc aagccctgcc cctaaagcta
    51481 atgttagaaa agaagcatta agtgctttcc tatttggaag accaaatgaa atatttgaaa
    51541 cacctaatat agtttttact cctagtgtct ttaccattgt tatagctttt atagtttcta
    51601 ttgcttcttc ttgttgtgct gaaacagtta atgataaaca gtctataaat atatcttttg
    51661 gttttatccc ataagaaact gctctattaa ctatcctttt tgctatttca aatctttctt
    51721 cagccttttt aggtatcccc ttttcatcaa gtgtaagtcc aaccacacat gaaccatact
    51781 ttttaactat tggtaatata ctttctaaag actcctcttt gccatttaca gagtttacta
    51841 ttgttctacc attataatat ctaagtcctt gctctaaggc ctctacattg gaagaatcaa
    51901 cttgtaaagg tgtatctact actgcttgaa tctctcttat taattttggc attagttttt
    51961 tttcatcaat ctctggaaga ccaacattta ctccaagtat ttgtgctcca gcatttactt
    52021 gttctaaacc taaatttata gcataatcaa cattttcatt ttttaatgct tcttgtagag
    52081 attttctacc tgtgggattc aatctttctc caacaactgt aggcgattgt acttccacaa
    52141 attttgatgg tgaacagact acacatttat caattttctc gatacatccc tttgtaacag
    52201 aatttatatt ctcagatatt tcttttataa aacttggatt cgtaccacaa catccaccta
    52261 ttatagtagc accaacttca acgaactttt taaccccttc aaaaaattct tttgcatcaa
    52321 catcatatat agcctctcca tcaactatat ttggaagacc tgcatttgct tgaaccataa
    52381 cagggactgt agctcttgat gctatttttt caactattgg taaaagttgt ttaggtccta
    52441 atgaacaatt aactcctatt gcatctacac ctagaccttc tatagtagcc accatacatt
    52501 ctggcataca tccagtaaaa cttcttccac cttcatcaaa tgtcatagta cagaaaatag
    52561 gtaaatcaga gttttctttt gctgctaata cagctacttt agcttcatat aaatccatca
    52621 tagtctctat tactattaca tcaactccag atttaacgcc ttgtagtact tgtcttttaa
    52681 atatttcata tgctttatca aaacttaaag tacccatagg ctctagcatt tctcctatag
    52741 gaccaatatc taaagctaca tatcttggtt tagtattatc tacattttct atagcttttc
    52801 tagccaccaa aacagcattg tcaatcactt cttctacagt atacttacta tctaatttta
    52861 gttcattaca tccaaaagta tttgtaagaa ttacattcga acctgcctct aagtatgctg
    52921 tatgtatttc tataagttta tctggatttt gaagaccaaa tacttctgga ttttcaccta
    52981 actttaaccc tttttgttga agcattgttc ccattgcacc atcaaatatt aatatattat
    53041 ttttcagata ttgtcttatt tccacaagag ttcgctcctt ttctatatgt acagtttcta
    53101 tagtttttac aattttcaca cgacttttct ttcttagatt tatttattct tgtattagaa
    53161 atacctataa tagccactac tgactttctc ggtatcataa ttccattaga agtaattgtt
    53221 agacctattt gtttttgagc attcaaaacg tttaatatat ctatattttt ttctattggt
    53281 aaatctccat atcctgggct atatcgcata gttataaatt tatcttgctt taaaaaaacc
    53341 ttttctaaat tattttgaac aatgtcacac acttcttcta ttgctgttgt tccacatgaa
    53401 tcaattatta gtccttttgt taaatttgaa taagagtgtc gtcttatttc tttttctata
    53461 tctatcccta tagtagctgc cataattaca cactcattac attcttttaa aagttcatat
    53521 acatctttac tctctaatgt taaatttgtt ccctctaatt ctacaacatt atctttattt
    53581 attttaatag gataaatcct agataaatat ctaggattta ttttattttt agttatagct
    53641 acacattcat caatagtata tttaaggtct ttatctatct cttgacctct atattgtaaa
    53701 tatcttaaaa cttcactttg atttatgctt atatcttctc ttccaattaa attattctcc
    53761 atatatattt aaagaaaatc ctttatctaa aacatctttt gcatattcct ttgccccaaa
    53821 taaagataag tctctgtagt ttccacattc tatagctgta gctgctggca ttttatctga
    53881 ttcaagaact ttttttaatg cttcttctaa accaatctta actgttttgg catctacatc
    53941 tccccatagt attaaataaa atcctgttct acatcccatt ggagataagt ctattacacc
    54001 atctaatgta tctcttaaat aagtagctaa aagatgctct aaaccgtgca tagcagcagt
    54061 tccaaaactt tctacatttg gctgtaaaaa tctcaagtca aatttagtta ctttatctcc
    54121 ctttactcca tcaagtacac tacactttct tacaaaagga gcttttactt ttgtatggtc
    54181 tagcttaaaa ctttctactt tttccattat ttaatcctcc attatacttt aaaatttgtt
    54241 acattaattc taaaaaaaac tcttcctatc tattattgat aagaagagta tattatttca
    54301 agtcatctca tcgttgttag caggaccacc tgataaaagg ttggtatagg atcactgagc
    54361 taactctctc ccctaactct tgataagtat cttattaaac tgacttaaat tatatattac
    54421 ttttttattt ttgtaaacac tttattgtta taatatttat atcacataat cgtactttta
    54481 ttttataact aagctgattg tattattggc agttgtactt caaacacagt ttgatattta
    54541 tcgcttgaaa cagtaatttc tccatcatgt aaatttacaa tattttttgt aatcgcaaga
    54601 cccaatccag aacctccaac atttctattt ctcgattttt caactctata aaatctatca
    54661 aatatatttg gtaaatctaa cactggaatt ggttccccat aatttatcac ttttacaacc
    54721 gccatatctt cctttttctc tgtgactata tctacataat gcccatcttt tccatatttc
    54781 acagcattag ttataagatt ttcaaatgct cttaccaatt tatttggatc agcttttaca
    54841 attaattttt cttcattaaa attaattcta ctaaccataa attcttgttt aaaataaatt
    54901 tcaaattgag atactacttg acttaataac tctactaaat ttatttctat tttgtttaat
    54961 tttattgtat tattttgcat tttagtcaat tcaaacaaat catttataag aacatttaaa
    55021 tttttcgatt tttcatatgc aatatcaaca taatacatca tctgaacttc atctttatac
    55081 ttaccttctt ctatcactcc taaataaccc attatggatg tcaaaggtgt tcttaagtca
    55141 tgtgacacat ttgttattaa atctgttttt gtctgctgag cttgtctctc ttcaatagtt
    55201 atatttctaa gttgatggac tatattattg attttatgtg ctagttcatc aatatcatcc
    55261 ttttctttat cctttaatat atctaaatca tactcaatat ctatactttt ttctaagtta
    55321 ccttcatcca ttaaatttac agcactttta atgatttcta atttttgatt ttttctatta
    55381 gtctttctta taaaatctac aatttgctta tttctcttgt acgatatcat ggaataaatt
    55441 gctaatgatg tgattgtaaa taaaaatgga taaaataaat cataccctat aagacttctg
    55501 aaatctcttg cttgatatac aagttgatta actacagaca tatcactatt gacatataat
    55561 gattttatta tatacatact tgttaaaata agcattgaag ttaaagcaca gcttattata
    55621 agatctaata tgggtcgaaa catatgattt aataaacttt tatttttcaa ttttatatcc
    55681 tactccccat acagtatgaa ttattttttc tccatctgta tatttttcaa ttttatccct
    55741 tattctgctc atatgaacca ttacagtatt attagattgg taatatttct ctttccatac
    55801 cttttcaaat atttcttctg tactatatac tctgccttta ttatttgcta gtaaatataa
    55861 tatatcaaat tctcttgatg tcaaaattat ttcatttcca gccactgtta cactatgttt
    55921 ttccttatct ataattacta ctccaacctt tatgacatct ttatcttcat tattagcacc
    55981 actaaaataa tatgctcttc taatcagcga cttcactctc acagtaagtt caagtgggtt
    56041 aaatggtttt gtcaaataat catctgcacc agtcattatc ccttgtattt tatccatgtc
    56101 ttctgatttt gcacttaaca taagtattgg tatattcaaa ctttctctaa cttttctaca
    56161 aacttctatc ccatccattt taggcatcat tatatccaat atcattaaat gaatattttc
    56221 cttttttaaa atatctagag cctcttttcc atcttcagct ttaattacat tatatccact
    56281 atttaaaaga tatatctcta taaggtctct tatttccttt tcatcatcca caacaagaac
    56341 tgtattattc atttttacac atcctaactt aatctttgta tatagcttta ttcatataaa
    56401 attatactat attttagtta gtgtaatcta taaatttatt aatttctaaa tttccaatat
    56461 aatacaaatt atttacacat ttatattatt tattttgtaa atattcttct aaagttaatc
    56521 cttttgaata aatttcctcg gcagcatcct taccaacata tctaatatgc catggttcat
    56581 atgctgtacc tgttatatct tctttacctt caggatatct tattataaat ccaaacttgt
    56641 gagcattttc tgccaaccat ttagcttctt ttgtacttcc aacaaaccat ctttctggat
    56701 ttgttaaatc aatacaaagt ccagtttggt gctcactgta accaggcttt gctacatacg
    56761 catctgcctt ttctcgcccc tgagatttta cccttcttgt ataagtatcc agttgagtat
    56821 cataagatct gtatgctgaa gaccctaaaa actgtattcc ttctgaatta gcttgcttaa
    56881 ctaaatcttc aactgccttt gcaggttctc ctgccatttg tttttcttcc tctgttgctt
    56941 cttcaacaaa aggtatgttt accttagtaa tattttcagg agtatagttt ttgctaattc
    57001 catttgtctt gtttaccaat aatatatttg atatactatt tgagtgattt ttattttgag
    57061 aatttgcctc aacatcttgt tctgaatcat gttgagcagc tactattgga tctttatctt
    57121 tcttaagtaa tctatcttta gatataaata cacttataaa tactactaaa ataatggctc
    57181 ctattaattt aaattttttc ataattaaat cttctccttt ttgttatgtt tgctataatg
    57241 ttaattttag agtataagac ttaagtattt tataagtaaa tcttaaataa atcttaaatt
    57301 taatattttt aaaggatggt gataaaatgt ctatacaaaa acttctaagt aaagctcgta
    57361 aggctataca agattttgat atgatacaag aaaatgataa aattgcggtt ggactttctg
    57421 gtggaaaaga tagtttaact ctacttcata tacttaaaag ttatcaaaga ttttctccac
    57481 aaaactttga attgatagcc ataacattaa atactggagg tgttgataat tctccactag
    57541 ataaattgtg taaagaaata aatgttcctt tttatgaatt tcaaactgac ataaaagaaa
    57601 ttgtatttga tataagacaa gaaaagaatc cttgtgcatt gtgtgctaat ttaagaagag
    57661 gtgctttgaa cgataatgct aaaaaacttg gatgtaataa agtagctctt ggtcatcata
    57721 aagatgatgc aattgagaca tttttaatgt ctatgtttta tgagggtaga gtaaattgtt
    57781 tttccccaaa gacataccta gacagacagg atttaacagt tataagacct atggtttaca
    57841 tagaagagta tatgactaag aaaatttcaa aagattctaa ctatcctatt atcacaaatc
    57901 cctgccctgc aaatggtcat actaggagag agtacataaa aaatcttatt gctaatttaa
    57961 ataaagagat gcctgatttt aaaagaaatg tatttggtgc tttaaataat tctgagaaat
    58021 tgtttatatg ggataaagag aagataaaaa attttaaata ataattaaaa tttttaaata
    58081 aaaaagcaag attgtattaa ttgtaaataa tctttatata atctacacaa tcttactttt
    58141 ttatttatta tatgttatta tctttaatct atattttaat agttcttaat agtatttatt
    58201 ttgtctaata cattaattat aacttaaata ttttatatat taatttaatc tattaattca
    58261 ttcaatctac tggtttaatc catttttaca gtatatacat ctacatatta taaaaagcaa
    58321 aattatattt tctaaatatt ttatgttaga ttataatatt aaattctatt atctctggta
    58381 tattaaaaaa tctaaatggt aactttgtac ttccaagacc cgaactcaca tataaatgat
    58441 ttccatttat gtcataaaac cctttagtat atttccttcc atatggagga gtaactaatg
    58501 ctcccttaat aggtaatctt atttgaccac catgactatg cccagataga actaaattca
    58561 ttgtatcttt actaagcata tctactaaat ctggttcatg tgctaacaat aaattaaaac
    58621 ttttattatc tatttgactc tccagatatt ttatattagg atttccattt aatatttcat
    58681 caacacctaa tatattaata tatttatcat cttttaattt tattttttta ttttcattta
    58741 ctaaaagatt gaagttggaa tttttcataa tctgcctata atacctagga agtttataca
    58801 tataatcatg attgccaaat actgaaaatt ttcccaacct agcattcata cttgataaaa
    58861 tagttgtaat ttcatctaca cttggatttt tattggagta atcaattaaa tcaccagtaa
    58921 atactattat atctggattc aaagtgttaa tcttattagc tacttttttg agattcttag
    58981 ttgaataaaa actacctatt tgagtatctg aaatttgaac tacttttaca ctttcagatt
    59041 tactctcaat tgaatcattt tttatcaatg ctccattttc acttatatta tattttttca
    59101 caattaatag actcggctct atcgcaactg agtatataaa aatcaaaata aatattaaga
    59161 taaaaaattt taaaaaatac ttaatttttg agttcaaaat atctacctac ttatttattt
    59221 ttttaaacta tttgcattac agactcaact ttgttcattt ttctgtcatg catttttggc
    59281 ttatttgctg aaaaccattt atatgttgct gtaagacctt cttttagtaa cacgtttggt
    59341 atataaagcc catgttcaat aagcttattt atatcaagat taaagtctat atttctaaat
    59401 gggaaatacg ttctttctct aaactctact ttttccatat ctacatattt tattataggt
    59461 tctttgccaa taatttctcc acatgtatat ataaggtcat cccaagaaat taactgtgga
    59521 tttgtcacat tataggcctc tcttacatga ggatttttca ttatgctttc caataccttt
    59581 accaaatccc caatatatat aaattgatta ctaacttgtt ttccttttgg tactggtatc
    59641 ttttcatttt tttctatttt ttcaaagaaa taatactctc tatataaatt attattttca
    59701 ccatatatgt aagttggtct aataattata tatggtatag ggctatttat aataaaatct
    59761 tcagcctctt ttttatttaa cccatatttt ccccagtttt cattttcacc tttctcattt
    59821 tcttctttta tatttcttcc actatcttta tatactgctc ctgctgacaa tactatatac
    59881 ttttttaagt tatccatgct tataaaatca atcagattac ttacatcttc ttttgtatat
    59941 gctgtcatat catatatgta atcatatttt cttcctgtga taatgttttc catatctttt
    60001 cgaacttttc tatcacaaat tagatgttct ttaaaaccat tataatctat ttccttttta
    60061 ccatttgtaa gtatatcaat ttgataccca catttaatca agcgcttagc aagtgcagaa
    60121 cctatgaagt cgcttccacc cataattaaa atagatttca ttttaagtgc atcctcctct
    60181 tttttaaatg acttccttaa ctataatatc atatttttaa atatgcgaca ccattttgta
    60241 atatttttgt ctataaaaga tatttataac aaaatatctt ttatagactc tacattttcg
    60301 acaatataag taggttcact ttcactaatc tcttcaaaag aaccataccc atacaatact
    60361 ccaatagaat ctatacctat ttttttagct ccaattatgt catactttct atctccaacc
    60421 ataataactt tatctttatc ttttacatta cataggtcta gtacatattg tataacttca
    60481 tttttattca ctcttgtacc atcaagattg ctacctgcta tatatttaaa atatctatct
    60541 atatcaaaat atctaagtat agtttctgca aaaactgttg gcttagaagt tgctactaat
    60601 aatattttac catttttata gagcatttct aatatctctt tcatattctc ataaatttta
    60661 ttttcaaata tccctttatc agcaaaatat tctctatact tttcaacagc ttctttagct
    60721 tttttatctt caaatttata atactccttg aaagtatcat gtaatggtgg tcctataaat
    60781 tggtctaagt tttccaaatc ttcttttatg ccaaagctat ttaaagaata ctgaatagac
    60841 tttgttattc cttctttagg gtctgtaagt gttccatcta aatcgaataa tactatttca
    60901 taattttttt tcatatttca gtcctccata tttgtaaaaa gtaatatctt atatattaag
    60961 tttatctatt ttaaaaagct atagtaatag aaatttctaa tactatagct taagtaccaa
    61021 ttaaatctta tatatttatt ttaaaaataa atttaaacag ctctatttaa ctgttatagc
    61081 tgaaactgga catccttctt cggcttcttt acataaatct tgtaaatctg ctggtatttc
    61141 tccttcaatt gctactgaat gcccttcatc gctcatacta aaaacgtctg gacatgttcc
    61201 tatacatgct ccacaagcta cacatacatc cttatcaaca aatgctttca tactaaaata
    61261 cctcccctaa aattattatt ttttaattgt tgtttcctta gatatttatc caatatatac
    61321 caatttaaac atatatttat tattttatca ctattgttta gtttattata gtattttcgt
    61381 atccatgtct ataaacttca ctgcttttca tccatgattt tacagtatat tgcaatctta
    61441 attttttatt taaaacaagt tcattattat tgtagtcttt tataatatca ttacactcct
    61501 ccattgtaaa tattgtaaaa tcaccttctc caccaatctt tattctgcct ttatttccat
    61561 gtaaatcgaa aatctttgtt gggtattcag ttactttttc tatacacttt tctagggata
    61621 tcccaagttc cataatttta tttataacat ttaaaagaga atagtttacg tctttcatgt
    61681 tttttatatg catatcagta cttattaggt caggctctaa accttgcttt attgcttttt
    61741 taaaaacttt aaaatcaaat tggtccactc catgacatac atcaaacaat actcctctat
    61801 tctttgcatt tattactgat tttcttaatg tgccagattc tttaaggatt ccatttgtac
    61861 tatttccata taccggtgta accacatctc cttttcccaa aatattcatt acttcatcta
    61921 tatatgatgg atactctcct atatatacca ctaagggtaa atttaatttt ttagcaactt
    61981 gctttgattt cttaattata tctataccac tttcattaga ttgaaattta ctcgctttta
    62041 cttttatacc ctttatcttt tctatatttt tttctacaat tgactcaacc ttgtcaaaat
    62101 ttataacatc taaataagtt gattcattca atatatttag tccctcattt gtaacattta
    62161 aaaaagaata tacttctgtt ttacactcat ccatatactt ttttttaaaa tcatagtagt
    62221 ttgagggacc agctgtacct gcatcaaata taactgtaga acctttttca attccaattg
    62281 aatcagaatc catacctgta ggtaattttc tcttataaca atgggtgtga acatctataa
    62341 atcctgggga aatatacata ttatcggcat ttattatctc gaactcttta tttgtaagta
    62401 atttttcttt tcttattttc tctttaaggt tcaatccaat agcatatatt ttcccatgta
    62461 aaaccaagat atctcttttt tcaaatttga taccatctgt tggagatatt attgtcccat
    62521 ttcttataag taaatttttc atttattcca catcctaatt acaatataat ttctctaata
    62581 gcttttctta cataaagaaa ataagcctaa aaataaaaat attttagact tattcactat
    62641 attatactat attaattata ttaaattaaa taaaatcttt aatttacttt aagaagcaat
    62701 taagtatttg ttgctcggct tcttcttcag taagacctaa tgtcatcatc tttagtattt
    62761 ggtctcctgc aatctttcca atagctgctt catgtactaa aagagcatct ggatgatttg
    62821 cagcaatttc aggtatagca ctaatttgag aatttcccat aattattgaa tcacattgaa
    62881 catgtgcaga acaaagggaa tccccaataa cacaaggatt aaacttttgc ttagaattat
    62941 cttgcccaac tgtacgagaa actacttgag cagaagagtt ttcaccctct aagataacct
    63001 tcatattaga ttctgcaact tgattaccat gagttaataa tttctcaaat acaactattt
    63061 tagctccttt acctaggttt gcatccattt ctcttttagt tgaatctaca cccttaattt
    63121 gaattgtttc catttcacaa tatgaatcct catccatatt gacaatagta actggattta
    63181 aaattctctt tccttctcca tcaccttcac cataatgttt ttctacatat tttagtcgag
    63241 ctcctttacg taagaaaaat ctatgtatac cattatgttc actatcatca caaccacagt
    63301 tatgtatacc acacccagca attataacaa catctgcatt ttctccaatt tcaaaatcat
    63361 tatataccat atcctgtaca ccagcctctg ttactattac tggtatatgt acattttctc
    63421 cttttgtatt tggctttact ataatatcta ttccaggttt atcagtttta ggaattatag
    63481 ttacattttc agttgaatgt ctctctactc cttgcccatt tttacgtata ttgtatgctc
    63541 ctaatggagt tccacgaagt tcagcaactt tttctaaaag atttaaatct attttgttca
    63601 tttaatttgc cttcctttct aattaattca tgtctttcat taattcacaa cttgaattaa
    63661 cttgacacat gatttcaggt aatatagcct cttttgttcc atggcttttt actgaaccat
    63721 cttgtagaac aattatttca tcagctaact ctaatattct ttcttggtga gaaattatga
    63781 ttatagtctg attagactct tcatgtattt tcttaaaact ttcattgagt tttccaaagc
    63841 tccaaaggtc tatccctgct tcaggttcat caaatataga aacctttaaa tctctagcaa
    63901 aaagagttgc tatctcaatt cttttcattt cccctccaga aagagagtta tcaacttcac
    63961 gatttaaata gtcttttgaa cacaatccaa catctgtaag gtattggcaa catacatcag
    64021 tagacaatgg ttttccatga gcaagtgtaa gtaaattttc aactgtcata ccttttattc
    64081 ttggtggttg ttgaaatgcg tatcctattc ctttttttgc tctttctgta atactaagat
    64141 ttgttatatc ttctccatct aaaattatct gaccactcgt agctttttca attcccataa
    64201 taagcttagc aattgtagat tttcctccac catttggacc tgtgataaca ataagttttc
    64261 ccctctcaaa tgaaaggcta acatcattta ttattcctag ttcctcattg ttagactcaa
    64321 cattaaagga aagatttttt atttcaagca taatacccct cttttctata aaatattaca
    64381 ttaatatcca ttcttttatt atatcagtaa tttttatata tcagcaactc taaggagata
    64441 aaatgtatat taaataataa aacaaagtta ttgaaaagta aaaaagatga gttttatgta
    64501 aactcatctc agaaatttag tatatcaact tatatgtatt taaaatttaa ccttctatat
    64561 actctaatat tattttttga ttatctttta acataatttc ttctttttta gattctattc
    64621 cattcttttt tatctttacc tcagctacaa aggcatcatt accttcaacc atttctaata
    64681 gttcatacgc ttttttacga cctattttag ttgctatatc ttttagtact gccattttct
    64741 taaacatttt atcacctcta ctcaaaattt acacttaatt tttaattata taattctaat
    64801 cctagactag tttataataa ttattgtctc taattatatc agatattgag taaatttaat
    64861 actttgtaaa attatttatc ctctctgacc aaataatcat ttccatcttg gtctttaaaa
    64921 gaaaacatcc ttccatatgg catctccatt aattccccaa cctcaacctc gttatttgcc
    64981 atttgttcat aagttccatt aatatctctt gtacttaaaa ttattgatgg atgagataca
    65041 tttatctctt tgttttgctt catcattaga tttttatcat acaacacaaa tgttgtaaat
    65101 tcatttttac ttggtgctac ttccacccat ttcatatttg gacccatttc cttttcataa
    65161 gttactacaa agttcaattt ttctgtccaa aataactttg catcctcttg attatttaca
    65221 tagagagtta tttttcctat tttatttatc ataacaatac ccacctttat tattaaataa
    65281 atacctaata ttttttatat ttaataatat acccatatat attattttct atagatatat
    65341 atttctaata tttatcgcca aattatgtaa ttctgctcaa tatataattt ataatatcca
    65401 tactattata gcctttattt atgtaattta atcaccttat atatcatact tataattatg
    65461 ataataacta cataaaaaat cacttctaaa tagagttatc catctaaaag tgatttcata
    65521 tattctatgc aactttgtgt tttagccact ttgttcctct taatcttaaa caatataaag
    65581 tccctctaac aagccaatca gtatacatac ctatccaaat tcctaacact ccaaattcta
    65641 atacaacccc caatacgtaa cctgtaaaaa ttctaaaaat ccacatacct attactgcag
    65701 tcatcattgt atatctagta tcaccggctc cctttagccc tgctgataat acaaaggata
    65761 taggccatat taccatagct aaacaatcac ttcgaattaa ttgtgttgag atgtcaatta
    65821 cactagcatt ttgagtatac atacttgaaa tccagggtgc aattggtata aatatagctc
    65881 ctactataaa taaacaaatt gttgcaaatt tagttaaata taataaagta ctcttagctc
    65941 ctttaatatc tccacgtcct atatactgtc ccaccaaggt tgtagcagac aaagctaaag
    66001 cattcccaat tacatttatt atggatgcaa ttgacattcc tattgcattg gatgcaattg
    66061 ctgatgttcc catagtaact ataaatactt gaacaatcaa tttacctgca ttaaaaagta
    66121 gttgttcaac tccagctggt attcctatat taaatataga cttttgagtt tcaacatcaa
    66181 tcttaaatgg aaatatttta cccatagata taaattcgtt tcctttaaat aaagctctta
    66241 taattactat acatccaatt attctagcta tagcaataga tatagctgca cctgttatgc
    66301 cataggatgg tgtttgcaaa tttattccta gaggatttaa atcaatacca tatataagta
    66361 tatatcccaa tgttacattt actatattca taaacatagt cacatacata ggtttcttta
    66421 catctccaca ccctcttaaa ataccatttg ctatttgttg tgtcgcaata aatggatacg
    66481 taaataatgt aaactctatg tataattttg catttgattt taccaattct tctgcggaac
    66541 cataaaataa attaattaca ccagttctaa aaacccacat caatattgtt attgtaaagg
    66601 ataatattac tccagacact agagcctgct taatagtttc attcaccttt tcactatctt
    66661 tttttcctat ttgttgagct acaacaacag tagctccaac agatagtgca gcaaatacag
    66721 atataaatat attgtttatt gaatcaacca ttccaactgc agatacagct tcttccccaa
    66781 tatgacctgc catcattgta ttaaatactc ctaaaagcat gacaaacgtc tgctctatca
    66841 ttataggaat tgctaacttt ataacatttt tccttataaa catccctatt cctcttttcc
    66901 ctctttatat aaatttaatt tttaattagt ttatcgaact gtaaattgac aatgcaaagt
    66961 ttagaattgt attgttcgca ttataatata ctgctttcaa ataatataaa tcttgtttaa
    67021 tatcatttga acacagtata tctttataat tatcttatgt ttattatttt ttatggatac
    67081 cacatttaaa atatttatta atttaatata aatattttcc gttattatta aaagtagcta
    67141 tatttcgtta atattgtatc actatcgtgt agcaataaca ataagtacat tttatatatt
    67201 ttttataata ttatttaatc atccaaactt ttaccacttt ttcaatatat aaaatattac
    67261 actttagatg taacaaaatc gcatccattt aaatattgta ttttatagac taattttagg
    67321 atgtgatact ttggttaatt taaatgcaaa taatctaaat gtaaatatgg tttatcctat
    67381 ttcttccaca gcacccatac gtttcagaag atatactgtt tcatatttag aaaacatgga
    67441 aaatatttat ttaactatag caacatactt tgatacactt ggtttaggca caatgaatat
    67501 tgacaaagtt tatggtcaat gggcttggtt tactgataat atatatgatt taaatctatt
    67561 tgtttttgta ggaaattatc cttatcaaat tgctaaaaac agatatgata catttgtaga
    67621 tattttgcca gtagctatta cttctattgt caatggagat agagtatttt taatttcaaa
    67681 tccttgtttg ttaaattcaa aaattacagt tagatttata tctagtcatc ctactcttaa
    67741 tagaacagtt gattatggca atattagaga ttttattttt taaaactgca acatgatgtg
    67801 ctattaaaaa ataccctaaa tttaatgata ttattaaata actagaatat ttttaattgg
    67861 cacatcatgc ctagctatta taaattcata atattatttt acttaagata agtactgtat
    67921 tttatctatt tgtatatttt tatttaccta gtttctcagt tctatatcct atttctttat
    67981 tagctagctt ataaccttct tgtatcattt cttctctcgt taatgcactc cctaataaat
    68041 tgtcaatact cagttgcttt ctatcactaa aatacttatc caataatgat tcctctatat
    68101 cttcctgata tgccaaattt ccttcatgca ttatatattt tacatcaaaa ccttctttat
    68161 ttaatacttt tcccaccaag atacttctat gacatcttat aggttcttgc atagctccta
    68221 ataaaactat cctataaccc atttgacaac ccttttttaa tctttctatt ccgtttaaaa
    68281 aaatatcttc ttttgctact ttctcaaaat ctgcataacc ttcttgcgta tatacatctt
    68341 tattagttct tttagctcca aattcttttc ccatgtatat atataaaaat ccggaatttc
    68401 taagagattc gttaaatgcc tctttattgt attgaatatt atatttagaa tatggagttt
    68461 ctctaatgtc aactacacaa tttatgtcat atttttttat catattaata agcttatcat
    68521 atggataatt tgaatgtcct attgcgaata tttccatata aacaccctcc tattctttga
    68581 aattagtatc tatatatgta gatacccgat ttacttaaaa ttaaataaag tattacttta
    68641 aatacccaat atcttttttt cttgatattg tatatttttt tactttatat ccttaaaaaa
    68701 atatacaact ttatatataa aacatcaata tttatatata ttttgtatat taaatacaat
    68761 tatttataaa gtctttaata aaaatttata aatagagcat ctttagaaaa ctattataat
    68821 aaaaaaataa gaggctgtct aaaaatatag ataagccttt tttcataatc gtaattataa
    68881 aaatatactt gatttcatat gaaaaagagt gtctcataaa ctaaaatgtt tactttgaga
    68941 caccaccttt tttattctta tttatacttt gtagacatat tataacatgc gtttcactgg
    69001 tatctgaact tctgtcaacc agtcttcttc actctcttta ttccatatac catctatata
    69061 agattctcta ggatttccaa ccactttata tttattatcc tctatccatt taaatgcatc
    69121 tgcgtaagtt tttcctaatg ttgaatatgg accattgtgt aatatacaaa ctgcattttc
    69181 aactgcatct aattctttaa attttaatat atccgtatct tcctttaaat catttatagc
    69241 ttgacacatt tcaacatcca tatcagtctc tctatattca ccatcatgat atatattaaa
    69301 acaataatca ggaactgcac atgtacaccc tgttttcatc atttcttcag ccattacatt
    69361 tggaaataga ttaaaaagtt catcataccc ctttaacact tttctcatag aagcaacttt
    69421 aacttttggt aaagacttaa taattacatc attcatattg acttctcctt tcaaattcat
    69481 aaggtaatta cgtagtttaa ttattttata ttcttcactt ttaattacat ccaacatttc
    69541 tttttcttta ttcattaaaa tattttcaac ttcttcagga ttttctacag ctttttttat
    69601 ctcagctaat gataaaccca tttgttttaa agttattatc tgatgtaatt taggtaattg
    69661 ctctgttgta taatacctat attttgtaaa ttcatctaca tattctggtt ttaacaaccc
    69721 tatatcttca taatacctta atgtctttgt agttatttta tttattttag aaaacattcc
    69781 tatagaaaac atctcatcac ctgcgcatta gatttgatta atttaagttt aaactttccc
    69841 cttaggttaa agtcaataca aaataaatta taattgtatt aattcttcaa ttctatctcc
    69901 actcaatacc aaaatatccc ctgtttttat aatggttgaa cctttaggta ttaaaacctg
    69961 ttcctttctt tttatcataa ctattaatat atcgtctggt atattagaat ctattatact
    70021 cttattagcc catttattac cctcatcaac atttacttcg ataagtttag agctaatttc
    70081 tccatcataa tcattaaatg tttttaatac aggtgtatta tcatcaacta aatccaactt
    70141 tctggctaca gttggtatca atgaaccttg tattcctact gaaagcaacg ctataaaaaa
    70201 tattatatgg aatatgtcat tctctatggc tactccttga gtaacagcat aaattgcaaa
    70261 aactactgaa gccgcacctc taagacctac ccacgatatg aataattttt ctttatttga
    70321 gaatttaaat tttgctagaa ctataaatgt tgctaatggt cttgcaataa ctatcatgaa
    70381 tatagatatg gccattgcta taccaaatac atttggtaac ttagaaggga aagataacaa
    70441 tcctaacata aagaataaca ttatttgcat aatccatgaa attccatcaa agaaatgaac
    70501 caaacttttt ttaagaggta tcctactatt tcctataata atacctgaaa tgtaaacact
    70561 taaatatcca tttcctccaa gatactcgct aaatgcataa gaaagtattg caatagctgt
    70621 tataaatata gtataaaatc cttctatctc gaagtttgaa tgtcttaaaa tataaacaga
    70681 taattttgct agtataaatc ctatagctaa cccaaaagct atttgtttta ttaccattgg
    70741 tgcaataagt tctaccccag aattattcat caatgtcaaa agaattagtg tcagcatata
    70801 cgctattggg tcattacttc cactttcaac ttctaataat gatgcaagtg aaccctttaa
    70861 gtttagtttt tgagaacgca acacagcaaa cacagatgca gcatctgttg aagctgcaat
    70921 tgccccaatt aataaacctt caagtaaact agttcctatt accatgtggc aaaacatacc
    70981 tgtaacccca gccgtaatta ttacacccag tgaagacatt aatatagaag gtatagcaac
    71041 aggtttagcc atattccagt ttgttccaaa ccctccaaaa aacattataa atattagtcc
    71101 aatagaacat aattgttttg tcagttcata gttatcaaaa tagattccta ccatcccatc
    71161 agacccaaat aacatcccta gtaagataaa aatcaataat attggtactc caaatttata
    71221 caataccttg ctagaagtta tacaaatcaa tagtactaac ccacttataa tcattaattc
    71281 tgtcatagca tcaactccat ttcatataaa ttttgtagat taatttacat ctacaatttt
    71341 attacttatt caaatataac atgacataaa ttttataagt tatggtacat aaagttcaac
    71401 aaaaatttca caatacatta aaatgtgagt gttatactta aatttatata tttgttttaa
    71461 ttatattcat ttaatattta taatttgaat ttttttttat taacatttca tttttcagtt
    71521 gattgtttaa tatatttcat tttttatagt aattttatat ataactacga aatataaagt
    71581 aattagataa gtaacaagaa actaacatgt aatgttagct actctagata tgaaatctta
    71641 aaacttttaa atattgaaat atttctttat atatattatt gt
//
LOCUS       DAYAOY010000007        65582 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYAOY010000007 DAYAOY010000000
VERSION     DAYAOY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.007"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            408..3050
                     /gene="adhE"
                     /locus_tag="ACGI6B_000527"
                     /gene_synonym="adhC"
     CDS             408..3050
                     /gene="adhE"
                     /locus_tag="ACGI6B_000527"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFG9749996.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3410..3751)
                     /locus_tag="ACGI6B_000528"
     CDS             complement(3410..3751)
                     /locus_tag="ACGI6B_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9749997.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3778..4449)
                     /locus_tag="ACGI6B_000529"
     CDS             complement(3778..4449)
                     /locus_tag="ACGI6B_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9749998.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4461..7037)
                     /locus_tag="ACGI6B_000530"
     CDS             complement(4461..7037)
                     /locus_tag="ACGI6B_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9749999.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7245..8180
                     /locus_tag="ACGI6B_000531"
     CDS             7245..8180
                     /locus_tag="ACGI6B_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9750000.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8199..8936
                     /locus_tag="ACGI6B_000532"
     CDS             8199..8936
                     /locus_tag="ACGI6B_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9750001.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(9121..14178)
                     /locus_tag="ACGI6B_000533"
     CDS             complement(9121..14178)
                     /locus_tag="ACGI6B_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFG9750002.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14203..14622)
                     /locus_tag="ACGI6B_000534"
     CDS             complement(14203..14622)
                     /locus_tag="ACGI6B_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750003.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14856..15107)
                     /locus_tag="ACGI6B_000535"
     CDS             complement(14856..15107)
                     /locus_tag="ACGI6B_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750004.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15196..15927
                     /locus_tag="ACGI6B_000536"
     CDS             15196..15927
                     /locus_tag="ACGI6B_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFG9750005.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15986..16897
                     /locus_tag="ACGI6B_000537"
     CDS             15986..16897
                     /locus_tag="ACGI6B_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9750006.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16948..17760
                     /locus_tag="ACGI6B_000538"
     CDS             16948..17760
                     /locus_tag="ACGI6B_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFG9750007.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17869..18561
                     /locus_tag="ACGI6B_000539"
     CDS             17869..18561
                     /locus_tag="ACGI6B_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9750008.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18648..19589
                     /locus_tag="ACGI6B_000540"
     CDS             18648..19589
                     /locus_tag="ACGI6B_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750009.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19626..20783
                     /locus_tag="ACGI6B_000541"
     CDS             19626..20783
                     /locus_tag="ACGI6B_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFG9750010.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20935..21495
                     /locus_tag="ACGI6B_000542"
     CDS             20935..21495
                     /locus_tag="ACGI6B_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9750011.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21523..22014
                     /locus_tag="ACGI6B_000543"
     CDS             21523..22014
                     /locus_tag="ACGI6B_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750012.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22243..22794
                     /locus_tag="ACGI6B_000544"
     CDS             22243..22794
                     /locus_tag="ACGI6B_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750013.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22880..23812
                     /locus_tag="ACGI6B_000545"
     CDS             22880..23812
                     /locus_tag="ACGI6B_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFG9750014.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23867..24730
                     /locus_tag="ACGI6B_000546"
     CDS             23867..24730
                     /locus_tag="ACGI6B_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFG9750015.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24977..26839
                     /locus_tag="ACGI6B_000547"
     CDS             24977..26839
                     /locus_tag="ACGI6B_000547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9750016.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26842..28308
                     /locus_tag="ACGI6B_000548"
     CDS             26842..28308
                     /locus_tag="ACGI6B_000548"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9750017.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28332..29168
                     /locus_tag="ACGI6B_000549"
     CDS             28332..29168
                     /locus_tag="ACGI6B_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9750018.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            29734..31317
                     /gene="asnB"
                     /locus_tag="ACGI6B_000550"
     CDS             29734..31317
                     /gene="asnB"
                     /locus_tag="ACGI6B_000550"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9750019.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(31382..31978)
                     /locus_tag="ACGI6B_000551"
     CDS             complement(31382..31978)
                     /locus_tag="ACGI6B_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HFG9750020.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(32005..32532)
                     /locus_tag="ACGI6B_000552"
     CDS             complement(32005..32532)
                     /locus_tag="ACGI6B_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HFG9750021.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            complement(32894..33892)
                     /locus_tag="ACGI6B_000553"
     CDS             complement(32894..33892)
                     /locus_tag="ACGI6B_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFG9750022.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            34569..35768
                     /gene="hadA"
                     /locus_tag="ACGI6B_000554"
     CDS             34569..35768
                     /gene="hadA"
                     /locus_tag="ACGI6B_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HFG9750023.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            35807..36607
                     /gene="hadI"
                     /locus_tag="ACGI6B_000555"
     CDS             35807..36607
                     /gene="hadI"
                     /locus_tag="ACGI6B_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HFG9750024.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            36622..37848
                     /gene="hadB"
                     /locus_tag="ACGI6B_000556"
     CDS             36622..37848
                     /gene="hadB"
                     /locus_tag="ACGI6B_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HFG9750025.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            37848..38975
                     /gene="hadC"
                     /locus_tag="ACGI6B_000557"
     CDS             37848..38975
                     /gene="hadC"
                     /locus_tag="ACGI6B_000557"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HFG9750026.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            39023..40156
                     /gene="acdB"
                     /locus_tag="ACGI6B_000558"
     CDS             39023..40156
                     /gene="acdB"
                     /locus_tag="ACGI6B_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HFG9750027.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            40189..40974
                     /gene="etfB"
                     /locus_tag="ACGI6B_000559"
     CDS             40189..40974
                     /gene="etfB"
                     /locus_tag="ACGI6B_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFG9750028.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            40993..42030
                     /locus_tag="ACGI6B_000560"
     CDS             40993..42030
                     /locus_tag="ACGI6B_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9750029.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            42513..44144
                     /locus_tag="ACGI6B_000561"
     CDS             42513..44144
                     /locus_tag="ACGI6B_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750030.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(44279..45205)
                     /gene="fba"
                     /locus_tag="ACGI6B_000562"
     CDS             complement(44279..45205)
                     /gene="fba"
                     /locus_tag="ACGI6B_000562"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HFG9750031.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            45535..45864
                     /locus_tag="ACGI6B_000563"
     CDS             45535..45864
                     /locus_tag="ACGI6B_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HFG9750032.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            45864..46586
                     /locus_tag="ACGI6B_000564"
     CDS             45864..46586
                     /locus_tag="ACGI6B_000564"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFG9750033.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            46613..47974
                     /gene="rlmD"
                     /locus_tag="ACGI6B_000565"
     CDS             46613..47974
                     /gene="rlmD"
                     /locus_tag="ACGI6B_000565"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFG9750034.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            48366..50435
                     /locus_tag="ACGI6B_000566"
     CDS             48366..50435
                     /locus_tag="ACGI6B_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9750035.1"
                     /translation="MQLTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSAL
                     MKLFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFF
                     KNMVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYE
                     LDQIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSII
                     LPNEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNV
                     LLYTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKD
                     KYISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGS
                     TYTEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMID
                     DSGGGRK"
     gene            50432..52297
                     /locus_tag="ACGI6B_000567"
     CDS             50432..52297
                     /locus_tag="ACGI6B_000567"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9750036.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            52421..52615
                     /locus_tag="ACGI6B_000568"
     CDS             52421..52615
                     /locus_tag="ACGI6B_000568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750037.1"
                     /translation="MKKKTKISKKIVSFIMVFITIVTTILTSSVSAYASELDMSEQTG
                     FNYLGISPITGKKLIITFIV"
     gene            52618..53235
                     /locus_tag="ACGI6B_000569"
     CDS             52618..53235
                     /locus_tag="ACGI6B_000569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750038.1"
                     /translation="MDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRKAE
                     ILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENTLK
                     ITANKNSNSGVISYIKIPKNEVGISIVYRKHNYQSLSEFHMESSMSANLNINVIKLGI
                     VKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            53227..53748
                     /locus_tag="ACGI6B_000570"
                     /pseudo
     CDS             53227..53748
                     /locus_tag="ACGI6B_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            54262..54489
                     /locus_tag="ACGI6B_000571"
     CDS             54262..54489
                     /locus_tag="ACGI6B_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9750039.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            54486..57425
                     /locus_tag="ACGI6B_000572"
     CDS             54486..57425
                     /locus_tag="ACGI6B_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9750040.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            57425..58099
                     /locus_tag="ACGI6B_000573"
     CDS             57425..58099
                     /locus_tag="ACGI6B_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750041.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            58260..58676
                     /locus_tag="ACGI6B_000574"
                     /pseudo
     CDS             58260..58676
                     /locus_tag="ACGI6B_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            58636..61083
                     /locus_tag="ACGI6B_000575"
                     /pseudo
     CDS             58636..61083
                     /locus_tag="ACGI6B_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            61223..61645
                     /locus_tag="ACGI6B_000576"
     CDS             61223..61645
                     /locus_tag="ACGI6B_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750042.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            62183..63217
                     /locus_tag="ACGI6B_000577"
     CDS             62183..63217
                     /locus_tag="ACGI6B_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HFG9750043.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            63304..63507
                     /locus_tag="ACGI6B_000578"
     CDS             63304..63507
                     /locus_tag="ACGI6B_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFG9750044.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            63678..64838
                     /locus_tag="ACGI6B_000579"
     CDS             63678..64838
                     /locus_tag="ACGI6B_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HFG9750045.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            65097..65237
                     /locus_tag="ACGI6B_000580"
     CDS             65097..65237
                     /locus_tag="ACGI6B_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750046.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT        25227 a         7491 c        11124 g        21740 t
ORIGIN      
        1 tacaagtcaa aaataagtgt tattttataa aaatacgaca aaaattttat ttactactta
       61 tacaaataac atgcaaataa catataaaat ttaataagta aaaaaaatga aagatataaa
      121 ttatcatttt agtattttat ttagtcgaag taaattattc aaatgaaata tatctcattt
      181 agataaaaaa ttttagtaaa agtaaaaaaa ataatatatt tgttaaaaaa taaattgaca
      241 aaatattcag aaaaaaactt cttgacctaa gagagtaaaa taaataaaat ctaaggtata
      301 gagatgaaat ctataaaatt attttaagta taagttaaaa atatagatga tgcatttaag
      361 atttataaaa attagagatg cataattatt ttagggaggt ttttagtatg gagaaaaaag
      421 aaaaggtagt agaaaaatca aatgttgaag tatgtagtcc tgaaattgtc aacagtgttg
      481 aaacattaag aatgagatta gaagaaatta gattggcaca aaaagaattt gcaacattta
      541 cgcaagaaca ggtagataag attttcttag ctgcttcaac tgcagctaat caacaaagaa
      601 tacctctagc aaaaatggca gtggaagaga ctggtatggg tattgttgaa gataaggtta
      661 taaaaaacca ttttgcctca gaatatatct ataatgctta taaagatact aaaacttgtg
      721 gtgtcataga aaaagatgaa gcatttggtt ttacaagaat tgctgagcca gtaggtgttt
      781 tatcagcagt tataccaaca actaatccta catcaactgc aatattcaaa tccctaatag
      841 cactaaaaac tagaaatggt ataattttct ctcctcatcc aagagctaaa aactgtacta
      901 tagaagcagc tagagttgta catgatgcag cagttaaggc aggagcacca aaaggactta
      961 taggttgggt agatgttcct tcgattgaat taactaatgt tgtcatggca gaggcagacc
     1021 ttatattagc tactggtgga cctggaatgg ttaaatctgc atactcttca ggtaaaccag
     1081 ctgtcggagt tgggcctgga aatgtacctg caataataga tgagagtgca gatataaaaa
     1141 tggctgtaag ttcaatctta gtatcaaaat cattcgataa tggtatgata tgtgcttccg
     1201 agcaagctgt aatagtacca gaaaaaatat atgaagaagt taaaaaagaa tttaaatatc
     1261 gtggagcaca tttcctaaat aaagaagaaa ctgaaaaagt aggaaaagta gtaataattg
     1321 atggttcatt aaatgccaga atagttggac aaccagcaca tgtaatagcc aaaatggcgg
     1381 atgtagaagt tccaaaaaca gctagaataa taattggtga agtagaatct gttgaattaa
     1441 atgaaccttt tgcacatgaa aaactttctc ctgtacttgc aatgtacaag tctaaaagtt
     1501 ttgaagatgc agttgctaaa gcagaaaaat tagttgcaga tggaggctat ggtcatacat
     1561 cttcattata tgcagactct ataaatcatc cagagagagt agaaaagttt gtaaatgcta
     1621 tgaagacttg tagagtatta gttaatactc catcatcaca aggtggtata ggtgatttat
     1681 ataactttaa attggcaccg tctcttactc ttggttgtgg ttcttggggt ggaaactcag
     1741 tatctgaaaa cgttggagta aaacatttgc ttaatatcaa gacagtagct gaaaggagag
     1801 agaatatgct ttggttcaga gcaccagaaa aggtttattt caaaaaagga tgtttaggtg
     1861 tagcagcaag agaatttaag gatgttatgg ataaaaagaa agcatttata gtaacagatt
     1921 ctttcctata taataatgga tatactaaga aattaactga tttattagat gagatgggaa
     1981 ttaagcatac tacattcttt gatgtagctc cagacccaac attagcttgt gcaagagaag
     2041 gagctaaagc tatggctgat ttccaaccag atttaataat agctgtaggt ggaggaagtg
     2101 caatggatgc tggtaagata atgtgggtaa tgtatgagca tccagaagta gatttccaag
     2161 acttagctat gagatttatg gatataagaa aaagagtata tgtatttcct aagatgggtg
     2221 aaaaagcgta ttttgctgct atcccaactt cagcaggaac tggttcagaa gtaactccat
     2281 ttgcagttat aactgaccaa gattcaggtg taaagtaccc attagcagat tatgaattaa
     2341 tgccaaacat ggctataatt gatgctgata tgatgatgga gatgccacca agattaacag
     2401 cagcatcagg tgttgatgct ttaacacatg cattagaagc atatgtatct atgttaagaa
     2461 ctgaaccagc tgacggtcta gcactacaag caggtaaaat aatatttgaa tatttacctc
     2521 gtgcatataa aaatggaaag aatgataaag aagcaagaga aaaaatggca atggcatcta
     2581 ctatggcagg aatgtctttt gcaaatgcct tcttaggaat atgtcactct ttagcacata
     2641 aattaggtgc attccaccat gttcaacacg gtgttgcaaa tgcattatta ataaatgaag
     2701 taatcaagtt caattgtgca gaagctccaa ataagatggg tgcattctca caatacagat
     2761 atcctgattg catacaaaga tatgctgaat ttgcttcttt tgcaggtatt aaaggaagta
     2821 cagaccaaga aaaagtagat aatcttataa aagcaataga tgaactaaag gcaaaagtag
     2881 gtcttcctaa gactataaaa gaggctggag tagaagaatc taaattctta gaaagattgg
     2941 atgctatggt tgaacaagct tttgatgacc aatgtacagg tgctaatcct agatacccat
     3001 tgatgagtga attaaaagag atatatctaa aagtttatta tggtaaataa tatctattgt
     3061 gcaacatagg ttctattggg tttgtatata aattattaga tttgaatata aatagagttt
     3121 tattagagac acgtagaagt aactataaat aatagattat aatttaaatt ctaaatggaa
     3181 atgataaaaa gtcagtaatt taatggtaat ttcgaatatg tgtatttaat caattaactt
     3241 taaaattagt cggaaaattt ttttattaca tataatttta aagcataata tataaaaaac
     3301 tttatgaaaa aattataaaa atattcagaa aattctattg actaatttat ttatcatgat
     3361 ataataagtt tagaaaagaa aaaaattggg gcctattaag gcccccttac tattttttga
     3421 tagatttatt taaaattact ttctcatttt gattttttat atttattata gagtcagtat
     3481 aatccacttc aaagttggca aaagcagaga ataaatctat atctcttaac aaatgaattg
     3541 tcatacattg attgttctta tttagcctta gaatcaatgc tcttacaaat ggaaattgta
     3601 ttctagaatt aaataagtta gaaccatcag tatctatatc taagctacga taattaatat
     3661 ctataaatat atctacatct tcattttctt ctcttattat tctgtagtca aaatccaagt
     3721 ttaaatttaa attacctaaa atcaataaca tttaaatcct cttttctata aatagtttta
     3781 gagtgttaat ttaagctctc catctattat agatataatt ctatctgctt ttctagcaat
     3841 attttcatta tgtgttatta ttataagtgt ttggttatat ttttttatag aactttttag
     3901 taaatccatg acttcttctg tagttttact gtccaaattt cctgttggtt catctgctag
     3961 gattatactt ggttttgcag ataaagcacg tgcaatagca actctttgct gttgaccacc
     4021 agataattgg ttgggaagat gatttttcct ttcattaaga cccaataaat ttataaggtc
     4081 atcaatatat tctttatcta tttttttatt gtctaacttt agtggtaatt ctatattttc
     4141 ctttacattt aatacaggaa ttaagttata ctgttgaaag acaaatccta ccttttgtct
     4201 tctatatctt gctaattctt tatctgaaag acaacatata tctttaccat ctacctttat
     4261 agtaccatta cttggtcttt caagaccacc tactaaatgt agaaatgtac ttttcccact
     4321 tccacttggt ccaattatag caacaaattc gcctttattt atttttaaat ttgcattctt
     4381 tagtgcatgt acagatgttt ccttttttcc ataaatctta cctaagttaa tagtttccaa
     4441 tataggcata attattcctc ctattctgta atgtttatag cttcaactat actaatttta
     4501 tttatctttc ttgatggaat atatgtagct agaataccta caattatatt tgctacaaca
     4561 actagtatat atattttgta atctattgaa aatcccaatc catatgatac aaagccctgc
     4621 atataataca ttttaaattg tattataatt cctgacacga tagtaattat acttgagagt
     4681 acaccatata atattccttc ataaagtatc atatttttaa agcctctctc agagatacca
     4741 atagctctta acattccaaa ttcactagtt cttgaagtta agttataact tatgttgttt
     4801 attatgttaa gtacactgat tatgaacatt attgctacta taccataact ataaatcata
     4861 gttctagatg tcatcttatc agaattttct ttttctacca tcatatctac tgttgtagtt
     4921 ccaggaactt cacttccaat tttacctaaa aggttattta tttttttctt gtttgcattt
     4981 tcattaaggt ctgcataaac taagttatac ttatctacac cagttaattt ttttaggtat
     5041 tcgctactcg ttattacatc tacaccatca tctccagagt acataccatt atctgcaaaa
     5101 ggataactta taatagctcc aactttaaat tcatattcta agtattcata attatctttt
     5161 agactttcat aaagacctgt atccatccca ccctttggat atttgacttt tatagtatca
     5221 cctactttta tatctacagc tggtgttcct tcaccaggag taaaagtctt tgtgtatttc
     5281 tgtgttttag aaatttctgg tacatataca atagctaaat tttctttttt catttttttc
     5341 aagtttatat ttccacttac taagtatttt tctaatgact ttatcatctc atcattataa
     5401 ccttttagct tttgctttag tgtatatttg ccagtagtct tattttttat gaacagagga
     5461 tttagcttat aataaggagt tgaatttata gcttcgtagt aatctaatct atttatttta
     5521 tttacctcta ttcttgtgtt taagatacta gcagttttga ctttgtcaat tccatctata
     5581 ttttttattt tatctatctg attatcatta aaccctttta tatctttaaa agaagtattg
     5641 ttaccaattt cattgagata aacatttgat agaataaaat ctgcattcat atatgtttgt
     5701 tcttgagtag atttgttttg cacatctaaa aaagaatatt tataattgtc ttttatgacg
     5761 attatacctc ctatactcat agataataca attataatga accccttttt atttctaaag
     5821 atgtttttcg cagatataga ttttgttact gacatattta aagttaattt tgatattaaa
     5881 taattaactt tggttacatt tttatcagct ccaaaagttt ttctgatagc atcaatagga
     5941 gaaattcttc ttatttttaa gtatgtaaag aaacttataa ttagtattat tagtattgta
     6001 catgcaattg ataagagaat tattttatct ggaataacaa aaggtgtaac atttatattt
     6061 ccttcaaatt gaatatttcc tacagaccta ttaaatattt gtgcacctcc catacctata
     6121 catataccta taggcattgc tattagagca agtatgaaaa gttcataaag cataagtttg
     6181 aaaatcctaa agttggtgga accaatagct cttaagacgc catattcctg tactctttgg
     6241 tatattgata taacatagat actatatatt acaataccag caaatagact catagcaatt
     6301 gcaacgtatt tacttgaatt atctaaagta ccgtttttca tagtagattc tataagcatt
     6361 gagtttcttc ctatactttt ctcatctaac atagcatttt ttattatatt gtctatgttt
     6421 gtatttatat cactgccttc attaaattca atataagttg taaaatcatt cagttctgat
     6481 tcattgagat tagaaaaaat ttcacattgt cccttttttt tctctataaa tctatcttca
     6541 aggattccaa caactttaaa agtttctgga ttttcttttt gatataattt aaaagtaagg
     6601 ttttgattta tctctggttt taagcccata ctatttaaaa tccatttctc gactaccacc
     6661 tcattgctag cttttggaaa tcgacctttt ataagtttag aatgactagt caaataattt
     6721 ttatttgtat atagtatgtt tataggtaat ttttcattaa catctgtaga cgcatagtaa
     6781 gatgttatac ccagattttt aatatctttg ctagatttta tggactttaa ttgatttttc
     6841 tttatatttt taaagtatac atggtcagtt ccaagctctc ttttcaatct atctacatct
     6901 gcttgctgag cacttcttga gagagtacca ataccaacaa cgagtgatgt agcaagaatt
     6961 atactcacaa caattgctaa agacctagct ttataacttt ttatatacct tagtactatt
     7021 ttcaaattat ctttcatatt taccacttcc tttcttaact aaatcaatta tatatttatc
     7081 taaaatatat agcaaagtaa gtattttcaa attttaagta gttaattgta caaattcatc
     7141 tgtattttta caagtttaat taatatatat gtaaatttta actttttaca taattttttt
     7201 aaaatgatat aatccctata taaattgtag aggggagggg aatcatggat aacaattttg
     7261 tatttcctat agtaatacta actttgttta tatttagcaa tcttataatg aataagtata
     7321 aattttacaa agaattagac atattaaaaa gaggcatatt aacatttttt atatcgtgtt
     7381 ttgcattgat tttatttatg gagggcacta aatatataca attaaggtta ggatatagtt
     7441 ttttacttga aggagtatta aatattatac tttttttggt tttaattatt ttaagttttt
     7501 atacaggctt taaagttata agattgataa ataataataa tgaacaggaa tataaattac
     7561 aaaaactaga tagtaaaata aatcataagg ataatataat tatcaaatta agaagtcaaa
     7621 aacatgacta tttgaaacat ttgcaagtag tgtattctct attaaatact gggctagatg
     7681 aagatgccaa agaatatata atgaatatat caaatagctt taaatcaaaa aattcaaggt
     7741 atggaaaagt atcttattta gatgctatag cttcattaaa atacgaggaa tgtcttgaaa
     7801 aggatatatc ttttgatgtc tacattcaag attttatgga taatttagag attgaaccta
     7861 atgatatgag ttcagtacta ttaaacataa tagataatgc aatagagtct ttgtctacta
     7921 taaaaaaaga aaagaagtat ataagattac atgcatatga agatgaattt cagtatatta
     7981 tagctataaa aaataatggt cctaaaatac ataatgttga aagtatattt tggaatggat
     8041 ttagtactaa agaaggacaa gataggggat atgggctaca tttagttaaa aatatattag
     8101 ataagtatga ttatagtata cttgtaaata gtgatgattt tttaactgaa tttgtaatct
     8161 taatcccaaa atattattag ttaggaggag aaaagtctat gataaaagtt ttgatttgtg
     8221 atgatgataa gattgtaaga gatgatatag caaatatagc taaagagtct ggatatgtag
     8281 attatgttaa aaaagttaag gatggtatag aggcaattga atttataaaa aatgagaaaa
     8341 tagatttatt gattattgac attgatatgc catataaaaa tgggattgat tctgctgaag
     8401 aaattctttc tatagataag gatatacata tagtatttgt gacgggtttc gctgattata
     8461 gtcttgaaag ctttaaagtg catcctatag attttgtagt taaaccattt aaaaaagaaa
     8521 aaatacttga ttcaattaac atagcaatag accatattaa ctcccataag attgctaata
     8581 ataattttat agaagacacc ctttttgtat ataaaataag gaagcagata catatgatta
     8641 attttgatga tatagttatg tttgagaaaa attcaagagc aattaatcta cacactaaag
     8701 atgaagatat aataaagttt tatgagaact ttgatgattt aaaaaaacgc ctacctccta
     8761 atttctttat gacccataga caatacatag taaatctaag atttatacat aaggttatac
     8821 ctataaataa atcttcatta gagataaggt ttataaattt tcaagaaaat gccatattaa
     8881 acaaatctct agaaaaagat tttttatata gattttatag ggttaaaaga ttctagtgaa
     8941 gatagaagaa aaatactttt tgtgtgatat aataagtacc gtaaagataa tgtataagag
     9001 aagtttatat aaaaattatt tgtaattatg atttaatcta tataaattac agtatagtat
     9061 atattggact ttctaatttt tttaagaaag tccaatatat tttagagata gtttttatac
     9121 ttataaatta cttaagatac tagggtcttt aatcttacta tcttcagcaa gtaatagtat
     9181 cttagacata atctcagcag ttttagggtc ttcatcagcg aatggaagga atatcttacc
     9241 tctactttga gaatgtatag gaagtatatt caaagcaccc atcccttttt tatgtacaat
     9301 accagaaccc atatgcacag aatactcacc taaattacct gctataagag catgagagcc
     9361 ttcaaaattg acattgttaa tttttaataa taataaaagt tctttagcaa ttgctatacg
     9421 catctctaca gtagaatggc tagtttcagg gtcaacacca ccagcatgag cgacacttac
     9481 aacaaggtct atatcacgca taacttctga gaatagaatt ggaggtatag aagctaattc
     9541 taaactttta taagtttttc tatcaaaaaa ttctactgtt tcaatagtag gaggctctat
     9601 atctgctggt gaaaaccaat ctgcaagtgc aaacatcttt gccactatat tttctttata
     9661 aaatactttc tgtaatccct cttcataatc gacagtccaa agctgatttc taagaagtgc
     9721 tacagttttc tttggctgaa tttggtgacc agcatatctg cgagatatag ttttttcaga
     9781 taattcatca ctgtttattc tataatactc acggaatact tgcttaaatg gttgaacaat
     9841 ttgcttacta aataaatcat gttggaactc actccactta ccagattcaa acaaatgaac
     9901 tggatgtgca agttttaatt tattttttgc cgtcaactta taagtttcac cttctatatt
     9961 gcaaataaag aacttagact tatcattata aacatatcca atattatcat cacataccca
    10021 tactagattt ttaatcaaag ggcagataac tggattttta tagatattgg caatttcttt
    10081 aactgtaaat actatttcat ttttcatagc ttgttctaaa ctctgttttg cacgagaata
    10141 ctgactcttt aaatcttttt ggacagattt aagttcaagt acatatggat gtttagcaat
    10201 agcttttggt atactcttta aagacttgtt gcctttttga actttagtag atgcttttcc
    10261 gttttcatca acatctatcc aaattgaagt accatcaata tcttttggtt taaatagatg
    10321 agcaacttca tttagttttt cagtctcaag agcccacatc atacgattta catctccaaa
    10381 tccagcactt ctagctagat tttctagcgc tatagtacaa gcttttgatt cactttcacg
    10441 acgttgtgca ccaaattgct tgctttcttt agcaaacttt tgaataaatt cataacggaa
    10501 agttgcttct tttaggtctc cttttttcat aggaagtaaa gagaaacttc tcaatttatc
    10561 ttgatttcta tttgtttcaa tttctttcat caacatgtct ttatcaagct taccaagtgt
    10621 agcatcagca tagagttgtg cacgtctatg agagttacca ccactagtta tatatttagc
    10681 tgacttgtaa agtatattga atctttcttc cccaatagtt ttatatgaat ctaaaaacca
    10741 atctacatca aatgcaccat caatgaaact acgtggtgaa ataggggaga atagagcaac
    10801 ctctgattct ttttgtgcag agaagccttc atttatatgt gcatggaagt accaaattgt
    10861 acttttcaga cccttccagt ttaaatattt ttcaattatg tttacccatt gaggggcata
    10921 catagcagat tctatcaatc ttaactcagt aatgtcggta tttttaagta aactttttaa
    10981 tttttttgaa tcatcttctt tagtaggata gcaattttta agtagagaac ttaaaacatc
    11041 tttttttgaa taattgctac catatgaata tcctctctgg aaagtttctt taccaagagc
    11101 gaccaaaata tttacaaaat aatttatacc ttcaaagtat tgtatattat tagctagatt
    11161 agacacttct gttggcatat cacctctacg tgattcaatt tctataattc ggtttgtaac
    11221 agtttttaaa ttatctttaa gccaagggta cttttctaat gtgtcgccag cccatctagg
    11281 atttgtcaat gttcttatta tatttgatgc gttgtatcca cttatcattt ctctattaac
    11341 ttgctcttca gtcattaatc ctaattcatg tgcacgagca aacacattta tgctagtata
    11401 gaaaggaaac ttatattcta gcaactgata atttgaaaac gtagtgtaga aaaacttagt
    11461 aaaagaaata tcatcataaa taaacgaatg tgctttatca agccaatagt ttaaaatagg
    11521 ggcatttaaa gcagggcagt attcattttg tctgtaatag tttttagtaa attcaactct
    11581 ttgtttttta cattgttcta tagtcaataa gtttctaagt ttatagtata tttctaaaca
    11641 atagttaaat atttctttat ggtcaaactc atttagtaaa actcttaaaa gttttcttgt
    11701 aagtccatta aatttaagtt tttcaagttt tgtatatatt ttattttgtt ttttagagtc
    11761 aaggacattt gaatatatag atgaaaacca acttttttct gtttcattat atccacatgt
    11821 acttatacta taaaataaga tatcaagtat taatttgtaa tctaagtcaa gttttttact
    11881 agtttctctc caaagctctg ctaatggata attatcaaga gtatcattag aaactttata
    11941 tttaatagga gtcaagttat aagtagaata acctaaaagt actttgtcta cattacctga
    12001 ccatctttca acttcatatt cataatctgc attttcagta ataagttcat caaatttttc
    12061 ataaactttt attattttat ttatatccgt atcgagaatt ttttttgctt ctttttttgt
    12121 tgctacagaa acagaatcct cacgagtaga taaacttata tttgagataa ttttattaag
    12181 ctttgatgag ctattagaat catctttaaa atcaaattgt tctggaattt gaactttgtt
    12241 ttcagggtta tatattccaa aaccattttc tttgcagtaa tcattttttg ctttacctaa
    12301 caaattatca attagtattt gaacttgaga tgtattcttt ttagatttat caatatcctc
    12361 tataagtaaa ataagcttat tatatagttc ttgtctagca gtattgtctt ttaataagtt
    12421 tacaatttca agacctccaa ggcatttttg agaatttgaa gattttatca gtgaagaagc
    12481 aatattttcc aaatttgaat catcttgttc aagtaaaatt tttattactg tttgtctaat
    12541 agagcttgtc tttaaagaca atagattggt tatcttatct aattctacat ctgttagctc
    12601 aagctttcta agtagactca ataccgtttc tctattggaa acactcttat cacttaaaca
    12661 ttccaataaa aattcacgtt gtctatctgt ctttggttca tttacaaaat tgactataag
    12721 tgaagctctt aaatctgaac tcataagatg acaattttcc ataagcacat ctataaattg
    12781 agcattcata tcgtatgctg ttaaagtcat catacattta agtatttcat gagaagaaat
    12841 ttcaatccaa gtccatggaa atacacttcc tgaaaatgat tttgtctttg aatttaaaat
    12901 ttctaaagta tgttttaaac tttcaaattg ttcttttcta gcatcaaagt ctttgtcaat
    12961 aggagtgttt gacaagtcaa aattagtttc tctgttttgc caactatagt agtagccaac
    13021 agaaccttct acatagaaat ttgtaattac ccatgctagt atctctaaat catcaccctt
    13081 aatgttttta gatgctatag aatgtttaaa atatgtactt tctgtatgcg ataaaaagta
    13141 aagagctaca agttttttat atttttcctg acaagaaagt aagttttcaa taagagagta
    13201 agagtcatat atgttgtaag ttgcagtagc ccataaggca aaataaattt caagattatc
    13261 attgctatta attgattcta tacggaactt ttcatcagtc aatgatttat atgaaagttc
    13321 taaacattta cgtattattc caggtttttg gtctgaaaaa ccaagtcctg tccatgtatc
    13381 caaggctctt ataacagaac taaatcgctc taaattgttg tccaaaatag tttttaacat
    13441 gtatattgat gcagaaattg taccctcatc taaactctct acaatttgct gtctaagtcc
    13501 ttcttgttgt ttggctgcca aaagaagttt agaaagcatt tcatagcatt tatcactatg
    13561 actcttcatg ataccaatta tcatttgtct tgtaagtaga gcagtttgat tttctccaaa
    13621 tattacttct tctaaagcac ttattacttt ttgatttcct ttgtctattt ctagtgctat
    13681 tttatcactc attatagagt catagtacaa gaccttattt ggaagagtaa gataatctat
    13741 aactgaaaaa tcataataaa ttaaatcaac atagtttgaa ataaacctta taatacctct
    13801 aaaataatcc acaagtttat ttgagcgata ccctttacga taatatcctc ctgaatatgt
    13861 ataatccatc tttcttttaa ttaattggca aagttcttct acttgttcag aagtaaatat
    13921 taattctaat gcatttttta aaggtccaga aaggagctcg tccatatttt taatttcgta
    13981 gtagtggcaa ttgtagtatt tctttatcat atttaagaat ttgtcttcag gttctctata
    14041 atagtatgaa ctattttcta tgcagtccca aagagacttt gctaagtcgt agtactgttt
    14101 tgaagaagat ttagtaaaat tatttttaaa attttcaatt attttaggat ttttttctac
    14161 tctagtacgt tctaacattt atgtacctcc aaattattta ttttaaaaca ttcttccagc
    14221 aagcaaggta agtcttataa aagtaaatat atctccactt ttcagattta tagaggaatc
    14281 gagtttttca ataacatcct catttataag cactttgtag attccatctt caaatgcctg
    14341 taacgctacc tctattgcct tttctacaga ttgtttattc tcattgtaaa tagagccaaa
    14401 tccaactttt ccaatatcag cttgagattc aatttcttca ttagttaaga agtttattat
    14461 tgatttttct gggattttct cattatattc aagaacattt ttagatacaa tttcagagat
    14521 agcttctttt aggcaattga tgttgtcaga tacatcaaaa ggggtaagtt ctagcatagg
    14581 tttgcgtttg ccaacagcct taactctaaa ataaatttgc ataaaatcac ctcgcaaaat
    14641 attaattatg tatttagaaa tttaaaaatt tatctatttg ttatattata tcattttgtg
    14701 aattattaat gtgaattatg gaattatttt acaaaaaata gaaaaaaagc tatcttaaag
    14761 tctaaaaaag caataaaaga taacttttaa ctataattat atagaattat aactgtacat
    14821 ttattaaatt ttaattttag aattattttt gataactatc tttttttatt tataaatttt
    14881 gctcctaaat attttttgat atcttcttca ggagaaaagt catcaataag tatttctttc
    14941 ccagtgtctt caaatactac aagaacttca cagtcacctt gtaagaaaaa ttttctaact
    15001 gtatcttctt taacttctct aacttttatt acattctttt tttctttttc aaagtctttt
    15061 gtgtaataac ttcccatcat attaattatt tttgtatatc tattcataaa atacctccat
    15121 atgcgattaa atttaaaaaa tatgttataa taattgtttg tattaactta ataattataa
    15181 caaggagtaa taataatgac tagtataaaa aataaagata ttttaaattg tatagattat
    15241 tccattaaaa ataagctctt tgacaaacta aatgatgtat ataagtcctt gccagtagga
    15301 aattgttctg gatgtggaaa ttgttgtatg gagtcagtag gtataaattt aattgagttc
    15361 ttgaatatat tttgttatct tgaagataga gttgatttaa aaaaaagctg tataagtaaa
    15421 atattagatt attattttga ggagtataca agaaagaacc catgtccatt taaagatact
    15481 aataatagat gtttaatata tgaagttaga ccattaaatt gtaggatgtt tggacattgg
    15541 aaaaaagagg attataataa aaatttagat aatgttattg agaaaaataa taactataga
    15601 gagttaatga aaaagcaata tggatttgaa ataaatgatg aagtagtaaa ttacaggata
    15661 gattattgtg atagatttat tccaaataaa gattatttaa gtaaatctaa gagattaagc
    15721 ttttttgatg agcttatgat attagactca aatatttatt caagtggaaa tatagatata
    15781 gactttaggg atagaggtat agttgaatat tttatagagt cattacttta tagaaattta
    15841 gcatataatg tgaaaataag gatatctaaa gaatacaaaa taagaaaaag aactatagat
    15901 agaataaaaa ggattgttct tgtatagttt tatattagta agtaataaat tgtatcataa
    15961 atattgtcaa aatgaggtga agaaggtgaa tcttacaaaa gtaaaaggaa ataccttttt
    16021 tataaaaggt ggcacgaata caggaatata tgtatttgaa gataacacag cacttatgat
    16081 agacccaggg ttatgtggtt caagacctag gagagtaaat aaagtattag atgaaaataa
    16141 tatacaggtt agatatatta taaatactca tggtcatgat gaccactatg gagcttgtaa
    16201 tcaatttaaa gaatattata aagatgtgtg tataatgtca tctgaatacg ataaattata
    16261 tatagaaaat ccagaattgt tttcaaagta tattgttggg ggaaagagca atgtatttat
    16321 ggataatatc ttaaaaaata aaatgttgga tgatataaaa atagataaat ctatagaagc
    16381 tggaaaaatt gaattaaaca atgaaatgtt tgatataatt gaatttcagg gtcatacacc
    16441 tggtagtata gctgttttga cgaaggataa tgtgattttt gttggcgatt tattgatagg
    16501 agatgaaatt ctatcaaaat acgatttctt gtttctatat gatatacaag aacaaataaa
    16561 ttcattagaa aaattaaaaa atatagattt tgagtactta gttttagggc atggcaagca
    16621 agttatatcg aaagaagatt cttataaatt aatagaaaaa catttgaatg ctttaaaaaa
    16681 atatttatat caaataagag agttgcttgt agaaccgaaa tctttagaaa ttttattaaa
    16741 aaacattata aataacaata atttaagcaa taattataaa gagtatcatt tttttaaatc
    16801 ttcgttagta tctgtcataa gttacttagt tgatttagaa gaaatagggt atatattgga
    16861 aaatggagaa ttgctttact atacaaaaac aaaataaatt atgataaaat aaagaaggtc
    16921 ataaaaaaca aataaagggt gagcaatatg gaatacgaaa aatggaatga gatactagca
    16981 ccttatgaac atgcagtaga ggaattgaaa ataaaattta aaaatataag aaaagaatat
    17041 ttagcaaaag gtgaatattc tcccatagag tttgtcacag gtagaacaaa aaaaatatct
    17101 tccataatat ctaagttaaa aaggataaat gcaaaagata tagaaactga aattgatgat
    17161 attgctggta taagaataat gtgccagttt gttgaggata tatatgctat agttgatttg
    17221 ataaaagtaa gaaatgatat gactataata ggagaaaaag attatattac aaattacaaa
    17281 gatagtggtt atagaagcta tcatgttata ataaaatacc ctataaactc cattgcgggt
    17341 tcaaaggaaa ttatatgtga aatacaaata agaactcttg caatgaattt ctgggctaca
    17401 atagaacatt cattaaaata taaatatgac cattacatac ctgagactct tgctgaaagg
    17461 cttagaagag catctgatgc tgcctttctt ttagaccaag aaatgagtga aataagagag
    17521 gacataatga aagctcaggc tatgtaccaa atgaagtcta tagctattag agatacttta
    17581 aatagaatac aagagttata tgacttgggt gatacacata aagctatgca gtaccaaaga
    17641 aaacttgata gaactgaaac agataaaaat ataactgaaa ttttggaatt aaaacaagaa
    17701 atagatatat tattagagca atataaggat actaaggtag atgataaagt agttcagtaa
    17761 aaactacttg tagaaaattt gctaaatatt aatggggctg cctcttaatg gaaaataatt
    17821 tctaaaaaga gacagcctat ttagtgtaat taagaaagga atgatattat gagtttgtat
    17881 gctataggtg atttacattt ttcaacatca gtcaataagc caatgaatat ctttggaagt
    17941 aattgggatg gtcatgaaaa gaaaattata gataattgga aagaagttgt taaggaagaa
    18001 gatatggtct tagtactagg agatacatct tggggaatta atttatccga agctaaaaaa
    18061 gatttagata taataagcaa acttccaggt cagaaaatac ttataaaggg aaatcatgat
    18121 tactggtgga caaccgttac aagtttaaat aagctatatg aagacatgcg ttttattcag
    18181 acaaattttt atgaatataa agattatgca atttgtggag gtaggggctg gatatgtcct
    18241 aatgatgtta aatttgatga gactgatgaa aaagtttata aaagagaaga acatagactt
    18301 agactatcat tagaatctgc tcgtaaaagt ggtcactcaa aaataattgt gattactcat
    18361 tatcctccta cgaatgataa attggaagaa tctttattta caaagttatt tgaagaatat
    18421 aatgttgaaa aggtgattta tggtcacttg catggtaaag aatcttttaa aatgggattg
    18481 aaggggattc gaaatggagt agaatataca ttagcatctt gtgactatac agaatttaat
    18541 ttaataaaag ttcacgatta aaaaacataa attggtaaat actataagat acatgattaa
    18601 aaataaatat ttgagggatt tcggttttga gaaaggagac acgacttatg aggtggatat
    18661 tagtttttat atttttatat cttattcctt tagtagtgat attcagaaat tacaagaatt
    18721 ttaagaggtc ttgtatctac agtagtattt atgtggtatt agctacaaca ataatgataa
    18781 gtaatattta tatgagtgga cttaataaaa taaaagagtc tatgtattat cacaattata
    18841 tttctgatga gacatatgat gaaaagtatg tatcaaattt taaggaaaaa gaaagtactg
    18901 atttaaaaca ggataataaa aagaatgaag aaatatcaaa taataaaaat ttaaacaaca
    18961 aacaagatag aaatatacaa aataaagaaa gtaatgaaga aggaagtaat gatataaaag
    19021 aaaagaataa ttctgatagt aatgtttcag tgaataataa atcagaaaaa caagaagata
    19081 atacaaaaga aaataataaa tatacaagta aagaaaaaaa tagtgaagat ataaaaaaaa
    19141 tagatatgga aagtatagga atttttaaga aagaaattta taatatagaa agagcagctt
    19201 tagttccaat gagagaatgt ataccttaca ctaaggatat agcaaaaaat ataactaaat
    19261 tatctagtat aaaaaaagat gttacaaatg ccagaaacaa gtgtaaagaa gttgtaaaag
    19321 aatatgaaaa tatgcagatt ccagaacttt ctaaggagaa gtatacagaa acattgagta
    19381 atgctagaga tgacgtaaag aaagcatatg agcttagaga aaaatctatg gaaagtgctt
    19441 tgaaattaat aaatactaaa agccctaaat atatagggaa gataacagaa tatcttgatt
    19501 tatcagataa gcagatagaa agttttaaaa atagattgaa cgatttaaaa gaagaaataa
    19561 ataattcaga aacaaataat attaattaat aagatttaaa gatataagtt aaaagggaga
    19621 ttatgatgaa taaacagagt ataggaaaac taaatatgtt ttttatgggg attactcaaa
    19681 gaaaccttga taatagggat ttttttattg agttagaggt gatttttaaa tctggaagaa
    19741 aagagtttaa aggaattgca atagaggaag ataataaata taaatttaat tttaaaggaa
    19801 aatcagattt atatacgttt aatgagttgc ttaagaatat ttctaaagag gcagaaaatt
    19861 atgatggatt agtatttaaa tatgttgagc gaggaactat tgttgtaatt gaaggagaca
    19921 ataagaaggt aaatgtaaaa tatttagata ataaggaaga agtacctaaa atagatgaat
    19981 ttacggcatc tcaaataaaa aacagagatt attatgtaaa agtaggacaa gcaaatgcac
    20041 ttttaaaaga aattggagta cttacaaaag atggtaagat aaaaaatgat aaaataagaa
    20101 aatacaatca gatagaccat tttgttgaat tgatagatag tattttaaaa gaaataaagg
    20161 ataaagactg tataactatc ttggattgtg cttgtggaaa atcttactta agctttgtct
    20221 taaattttta tataaaagaa gtattgaaga aaaactgtta ttttatagga atagattatt
    20281 cagatgtagt tatagaagca tcaaaaaata tggctaaaaa cttgggttat aaaaatatgt
    20341 catttataaa agaggatttg actaattata cacctaatag ggatgttgac cttgtaataa
    20401 gtctacatgc ttgtgataca gctacagata tggcaatagg tctaggaatt agagcaaaat
    20461 ctgaagctat agtagtagta ccatgttgtc ataaggagtt attagggcaa tatagatatg
    20521 aagctatgga accaatatta aaacatggtg tatttaaagc tagatttgca gatttaataa
    20581 ctgatggttt aagaacattg ttattggagg gcaatggata tgatacttct gtagtagaat
    20641 acatatctcc acttgataca ccaaaaaatt taatgataag agcgataaaa actaaaacga
    20701 ataatgacaa agcattaaaa gaatataaag aactgaaatc acaatttgga gtggaaccaa
    20761 ccttagaaaa acttatatat tagttaaaaa tgtaaaattt acgaaaagct tgcttaaata
    20821 tgcaagcttt ttggtattat ataggtatag tcatatccag agttttgatt tataattaaa
    20881 gctgtgctat aatatttgat taatcaaatc aataaatgtt acatggaggt aaaaatgaac
    20941 aaattgaata tatgtattga tatagatgga acaataacaa gcccttatca ttttcttcca
    21001 tacctaaatg atatctttaa taaaaatata actgaagcag agtgtataac acataattgg
    21061 gaagaattgt atggttcggg aataagagat gtgtatgcgg agtttaataa taagtatata
    21121 cactcatatg atgaagcaaa gattgtagag ggtgctacag aggtaattag cacattatct
    21181 aaaggacata atttatcttt tgtaacggct agacatgagt gtttaactga tgtaactaaa
    21241 aattggctat cgagacaagg attttcagat atagatgttt acttattagg aagtgattat
    21301 aaagttgaaa aggcaagaga actgagttgt gacatattta tagaagataa tcctttaaac
    21361 tcagtgcaac tagctgatga tggtgttaaa gtaatacttc tggatactaa ctataataaa
    21421 gatgttaaac atcaaaatat agttagagtt agtaattgga tagatattga aaagattata
    21481 aaacaaggta tttaaagatt tgtagattga aagggggagc atatgataga tttaaaaatt
    21541 gaaaatagaa aaggaaaaat ccatgtaaag agcagtgaaa taaaagacat tttagaatta
    21601 cgtccagact tcgaatatgt acaagatata tcaaatacta taaatcaaga aaatatattg
    21661 gtatttgatt gccagttgac aggaaattct tttgatatgg atgatttaga tatagaagaa
    21721 atattggaag agttaggtga agaaatagat gagtcttatt tcagtgtttt atttgaagat
    21781 gtaagagcgt atttaaaaga tgctactgat gagatagaag cagacttaca ggataattac
    21841 ttatttgata atttgagatg ttattttgat atatacaata taaatcaaga atttacagat
    21901 tttaagtttg tatttttagt ttcttttaaa gatataaaaa tttcttcatt agcaaaccta
    21961 gctaagattg tttctaagag acagcttata ggtgcatcta agttttatag ttaaaatgaa
    22021 atattattat aataaaagct agtaaccttg taaaaatata ggttactagt tttttattta
    22081 ttcattttca caaaatttca gaaaactatt tacattctaa aaaaagtaat gtatattaaa
    22141 cataatgtac attataatgg ataaaaagtt tctattaaat tactaagttt atttaataat
    22201 caaaataatt tatcttatat tagtatgtat tagggggtat ttatgcaaag tttaaatgaa
    22261 ataattgctg aaaatttaaa aaagataagg aaagaaaaac atcttagttt agacaagatt
    22321 gcacaactta gtggtgtaag taagagtatg ctatctcaaa ttgaaaagtg tgaggtaaat
    22381 cctactatat ctactttgaa gaaaataact aatgggctaa aaatttcatt tacatcattg
    22441 atggagaggc aggaaagtga catagaattg attcaaaaaa gtgatataga ccattttatt
    22501 gaagataatg gaaagtatat aagttatcca atatttccat ttgactccaa aagaagattt
    22561 gagatattta tgattgaaat agaagaaggt ggaaatttag attcaaatgc tgaacttcca
    22621 ggaacacaag agtttataac tgtttttagt ggcgaagtaa ccattaaaat aaatggagaa
    22681 gattacattg tatctagtgg aaattctata agatttaaag ctgatgtatc acatatatat
    22741 agaaactcag gcaaaggtat agctaaaatg agtatggtag tatattatat ataaataatt
    22801 taatggaatt agagtgatat ttagatgatt ttaggtatga agatatatta tatttaagtt
    22861 ataggagtgt gatttatgga tgaataataa aaaagcagtt tttttctttt gcagtgattt
    22921 aaagaaagac ccagtagcca gtaatgtgat aaaatgttgt gaagaaataa tggatttaaa
    22981 tcaaactgat attgttattg atgataattt tgtattagag tttaaggata aaaacaataa
    23041 cttatttcat tttgtaaaaa caaaagatgt aataagtcat aattataaat attatcttcc
    23101 aattttaaat agacatttta gagattatga ttttgcagga gttgtaaatt ggcatgaggg
    23161 agaaaatgct ccagaacata tcctaacagc tcatacaatt ggagatgttc caacaggaga
    23221 atttggaaat tcaaatccaa gatactttaa aaatctaatc aatgctattg aggatataaa
    23281 aaatgaaaat ctacttgatg aatttactac attaacagaa gcaactcact ggtctggaac
    23341 aacatatggt gaagaatcta aattaattac agaatattct gtacctatgt tggatattga
    23401 gataggaagt agtagtgata gttttaataa tagtatagca atacaggtat tggcaaaatc
    23461 attaattaga gtatttgatt gtgatgaacc tttgaagact ttgttatgtg ttggaggggt
    23521 acattttgag aaatctttct cagatattat aaaaaataaa gaatataaca tatcaattgg
    23581 acatgtattg ccaaatcaat ggattgtaag tggaatgtat gatgatgaat ctggatttga
    23641 gaaactagaa aagtgtataa atagcattga aggaggaatt gattgtatag tttttcatga
    23701 taaattaaaa ggtacctata aagagcaatg ccgtaaacta ggtgaaaaac ttaatgtacc
    23761 tgtatttaaa cataaaatat taaaaaatcc aaaagactta cctatatggt agtcttatac
    23821 agttaatata acaataagtc ggattaattt ttaagcgagg tcaaaaatga tattatctta
    23881 ttttgcatta ttaattagag tagtgctatt agcttttgaa agagtagttg taagattatt
    23941 aggtgatgaa gaaggagata tttataaaaa tatagcttca tcatttctat ttttctttat
    24001 agggggagta tgtttacttc catttagcat aatggataga gttagtgatt ggtcgttttt
    24061 aataccatgt tatataagta gtttagtcta ttcaattggt tcagtagcgt atgtaacgtc
    24121 tttagcaact ggggaagttt cacttgttac tccaataaat agtttaaatt cattgttttt
    24181 actgctttta tcagttatat ttttaggtga aggtttaagt ttagcaaaag ttgcaggaat
    24241 cataattatg attggtggag tgtttatatt aaaaaaagta agttcttttt taaagaatgt
    24301 ctctgtaatt tttaacaatt taccatgtag attaatgttt ttatacatat ttttgcagtc
    24361 agtaggaaga gtattggata aggctttttt tgtacaagta tctcctatat tatattcaac
    24421 tatactgtat tttttcgtag gtttaaattt atttatcttt ttagcattta aaaagaaaca
    24481 aaaaataatt tgtgagattt ttactaataa aaaggggctt tcaattttaa gtggtgctat
    24541 taatggatat tcttatttag cattattaat tgctttaaat aatctagaac ttagtatagc
    24601 agagccattt tctcaagtat ctatgataat aactttaata cttgctcatt ttatattcaa
    24661 agaaaatata aaagaaaaaa taccaggttc aattttgata ctaattggtg ggtggctact
    24721 gttactttaa ttgacataaa aaatgaatta taaaggtgct attagttagt atcagaaaat
    24781 ttaaaaaagt attgacaatt cttttaggca aatgttaata taataataca aaatagtata
    24841 aatagaagac atgttactgg taatgcaggc aggatcgaat atattgcttt gatttttcaa
    24901 ggtgtttata taggttctgc cttttttgca tcttgaatgg ccattcataa aaaattatgg
    24961 agaaggagat tacaagatga attataaaaa aactgccatt gacattattg aacaagttgg
    25021 tggttcaaaa aatattttag ctttagaaca ctgttcaact agactacgct ttaagttgat
    25081 tgatagaagt aaagtcaatc aagatgcttt aaaaaaagta caaggtgtta tgagtatagt
    25141 taatggggca caatttcaag ttgtcattgg aaatagtgtt gttgatgtgt atgatgaaat
    25201 tttaaaagtt tgccctttag atacaaatga tagtactgta aaaactgaag aaaaaataaa
    25261 cttaggaaca agatttatag aatttattat taatattttt caaccactga tttttgctat
    25321 tgcaggagct ggtattttaa aatcactgtt gatgttatta gcaatgttta atattttaaa
    25381 atcagattct actatatata cattattagt tgctatttca gatgcaacat tctatttcct
    25441 acctcttatg gtagctgtaa ctactgccaa tgtattgaaa tgtaatcgtt tagtagctat
    25501 tgccgctgta ggatacttat tgcttccagc aacaacaact gctttaagtg aaggtgttga
    25561 actatttgga tttacaattc caaacattgc ctataatgca caggttttcc cggctatctt
    25621 atgtgtgtct ttcttggcaa caatggagaa aatttttaac aagtattcac caaaaccaat
    25681 tcgaactttc tttgttccta tgatgtcttt ggcaattact gttccaatta ctctaactat
    25741 tttaggtcct ttaggatata acgttggtac aatttttaca accattattt tattcttata
    25801 taacaaaatg ggattcttag ttgtaggatt acttgctgct tttcttccat ttatggttgc
    25861 aacaggaatg cacaaagcac taatacctta ttcaattaat acaattggaa agttgggtta
    25921 tgaagcttta tatatgacat cttcattagc ccacaatatt tctgaaagtg gtgcttgttt
    25981 tgcagtagcc ttgcgaacaa aagatgaaac attaaaacaa acagcattat ctgcaggaat
    26041 ttctgccttg atgggaatta cagagccagc tttatatggt attaccttac aacataaaag
    26101 agcaatgtta ggtgttgtat tctcaagtgc aatttctggt acctttttag gattatttgc
    26161 agtaaaagga tttgtaattg ttggaccagg gcttgccagt atgagtatgt ttattgaccc
    26221 aaacaatgga aacaatttga tttttgcgag tattggtttt gttattgcaa tattaggctc
    26281 gtttattatt acactgatta tttggaagga agatacaaat acagtagagt catcaattgg
    26341 agatgttgtt gaagaagagc tagaagaaga aaaagaagta aatatcttag taactccagt
    26401 agaaggaaaa gtaatagatt taagtaaggt taatgatgaa ctatttgcaa gtaagacttt
    26461 gggagatgga gttgcaatta taccaaccaa tggaaattta tacgcacctt gtgattcaga
    26521 agttgtgatg ttatttgaaa caaaacatgc aattggttta agaacaaaaa atggggctga
    26581 gattttgatt catattggaa taaatactgt aagtatgaat ggagatggat ttaaaacatt
    26641 tgttaagaca ggcgataatg taaaagaagg agatttatta attcaatttg atttagacaa
    26701 aatctcacat gctaatttag attcaactgt tatgattgta aacaataatg gagcagatta
    26761 tgcttataaa gtgttaaacc agtcttatgg caatgtaaaa aaaggaagta tcttgtttga
    26821 tgtaaaggga ggaatataaa tatgagaaaa tttccagaag gatttctttg gggaggagct
    26881 acagctgcta atcaatttga aggtggttgg aatttaggtg ggaaagggtg gtctgtatca
    26941 gatgtagcaa aggcacactt tgatgcagat gtgaaagatt ataaatctaa taatgaaatc
    27001 acaacaaaag atatagaaga aggattagca catcctgaag atgaagtaaa ttatccaaaa
    27061 cgtcatggta gtgattttta tcatcactat aaagaagata ttgctctaat ggcagaaatg
    27121 ggatttaaga catatcgaat gagtattgct tggtctcgta tttttccaaa tggagatgat
    27181 aaagagccaa atgaagaagg tttacagttt tatgatgatg tatttgatga gttgataagt
    27241 tatggaattg aaccacttgt tactatgtca cattatgaac caccattaaa tatcgtttta
    27301 aactatgatg gatggtattc acgacaagtt atcaatatgt ttgttaggta tgtagaaact
    27361 atctgtgaaa gatataaaaa caaagtgaaa tattggctta catttaatga agttgattct
    27421 atgattcgtc atccgtacac tactggtggt ttagttcgag accgttttaa ggataaaaac
    27481 tttgaggaag ttatttttca agctatgcat catcaatttg tagctagtgc cattgcaaca
    27541 aaaatttgcc atgagattat tccaaattca aaagtagggt gtatgttaac aaaattaacg
    27601 tattatccat atacatgtag accagaagat gttttggcaa ctcaacaaaa aatgagaagt
    27661 atttatgcat atagtgatac gcaggttttt ggtgagtatc cagtatattt actgtcgtat
    27721 tttaaaaata ataacattca aatagtaaaa gaagaacatg atgatgaaat tatgaaaaag
    27781 tatccagtag attttatttc attctcttac tatatgtctt cttgtgaggc agctgataca
    27841 actggattag atattacacc tgggaatacg ttattagctg taaaaaatcc ttatttagaa
    27901 atgagtgaat ggggttggca aattgattca ataggtctac gtatatcttt gattgaatta
    27961 tatgatagat acagaaagcc tttatttata gttgaaaatg gattgggagc taaagatatt
    28021 ttaactgaag ataaaaaagt tcatgaccaa tatagaatta attatttaaa agaacacttt
    28081 aaatgtatgt tagatgcgat tatcgaagat ggcgttgaac tttggggata tacttcatgg
    28141 ggttgtattg atttggtcag tgaatcaacg aaacaaatgt caaaacgtta tggatatatt
    28201 tatgtggatg ctgatgacta tggaaagggg acatataatc gatataagaa agatagtttt
    28261 tattggtata aaaaggtaat tgaaaataat agtatagatt ttaaatgacc aatagaaggt
    28321 gagcaaataa aatgaactac ataataaaga aagttttgaa ctcgagcgtt gttttagttc
    28381 atgatgcaaa gtataatgaa tttattttgc tagggaaagg aattggatat ggtaaaaaaa
    28441 caggagaata tatttgtggt tccaatgata atcaaatgtt tgtccctgtt gaaaatacaa
    28501 aatcaaaaca atttttagaa ttaatggatg atattcctgt cgacatttta aagataacac
    28561 aggaaataat agtagaggca gaaaagttat tgaactcatc ttttaataaa aacttatact
    28621 taatattagc agaccatttt aactttgcaa ttgaaaggat gcgtaaagga ataaaaatta
    28681 caaatcgagt attttgggaa ataaaaaatt attatccaaa tgagtttaag gttggaatgg
    28741 ttgcaattta tatggtagag gagagattgg gcattcaatt accagaggaa gaagcagcta
    28801 acattgcttt ccattttgca aatgctatgg cttcagatgg aaattcatat gatacgatta
    28861 agtatgccaa agtaattgga gaaataatta atatttttgt tttttctttg aatagaacct
    28921 tagataaaaa gagtatgcat tatatgagat ttataacaca tataaaattt tttgtggaaa
    28981 gatttttttc agatagtatg ttaagcagtg gtgatgattt attatttaat caaatgaaaa
    29041 ggtcaaatcc aaaagaaatg ttaattgcat taaaagttag agatttttta gaaaagaaat
    29101 atggaaaaaa gttaacaaat gaggaaatag ctttcttggt tgtacatatc gcacgagtta
    29161 agcaatagag tgaattacta atattatctt atacgtctac atggtagaaa attgaaagaa
    29221 ggtaaagtat ataaagaaac tgagacatat tattttgttg ttcattatgt ttattttatg
    29281 agtgtatcct gttaaaaatt taagttaata tttataatta aaatatacaa atagttaaaa
    29341 taagtttatg tggttatgaa tttattttaa ctattttttt gtttaatgga aaatatgttt
    29401 tataactaga attttatttt taattaatta taatacaaaa aactttataa aatcttcaga
    29461 atttcttaaa gaatatgtaa gtaacataag atatattata tttagttggt aatataaagg
    29521 gggcaatatt tatgaagaat aaaaaattca atgttataac actgataata cttgataatc
    29581 atatttatgc cattaataaa gagcagatta gtactaattc aaaaagaatt tactttgaaa
    29641 aaagtcgatt tttcagtgat tataagtgaa taaatatata taaacattta ataaatttag
    29701 aagttaaatg ttttaatacg aaaggataca catatgataa atttagaaga aaagtattta
    29761 acacgaaaag acacatatat gataaaaatt ctagaataca atgggcatat tcgaaactgg
    29821 aaagttcttt gtgaagaact tggaatcgat tcttcattat gtagagatga gcgtgaacga
    29881 gcaattttag tggaagcata taagacatgg ggatatgata tggcaaatca catgcatgga
    29941 atgtttgctt ttgcattatg ggatactgtg gaaaagaaac tcttctgcct aagagaccag
    30001 tttggggtaa aacctttcta ttattatgag actgaaaatg ggaagcttct ctatggttca
    30061 tctattcgta agattatgga gcaaccagga tttgtgaaag agttaaatga agacatgtta
    30121 cagatttata tgtctttcac atatgtagca ggtgaaaata cattcttccg tggagtaaag
    30181 aagcttatgc caggacatta ccttgtctat cagaataaaa cagtatgtat tggtcgttat
    30241 tggaaacctg aatttcatcc tgaccacagc aagtctattg aggaatggac ggatgaaatt
    30301 catacaacac ttcagaaaat tatgccagag gtaaaatcag agaatgaaac tgcagagtta
    30361 ttcctatcag gaggtgttga ttcatcttat atattggcaa tgtcagatat agaaaaaaca
    30421 ggttcatgtg gttatgagga agagcgtttt gatgaatcaa aattagcaca acagacagct
    30481 aatattcttg ggtgtaaaaa ttcgagatat ataattacac ctgaagagta ttttgcgtca
    30541 gtaccttatg taatgtacca tatggagcaa ccaactggag atgcatctgc tatcgttttt
    30601 gctattggat gcaaggcagc agctgaaaac acaaatatct gttactctgg agaaggagca
    30661 gatgagtttt ttggtggata taatatatac cataaggcag agtgttatgg agagaatttg
    30721 aagacatttt atattggaaa tacaaacatt atgaatgagg gtgagaaaag aaaaattctt
    30781 aagaaataca atcctgatgt gcttcctatt gaagtggtaa aaggaattta tgaagaaaca
    30841 gaagaactag atacacttac taacatgtca aatgtagata ttcaaatatg gttggaagga
    30901 gatatttatt ttaatataga taaaatgagt actgcagtag gattggaagt cagaatgcct
    30961 cttacagata caagaatatt tgatattgca tccagaatac catcagaata taaagtgaaa
    31021 ggaaaagaga ataaaattgc ctttcgtaca gctgcatcaa aaacattacc agaagagatt
    31081 gcttttagaa ataaattagg atttattgtg ccaattcgta tatggatggc tgatgaccga
    31141 tataataagg atatccgaga taaattcaat agtgagatag cagataaatt ctttaacata
    31201 gatgagatta atgagatttt taatgactac attagtggaa actctgataa ttggagaaag
    31261 atttggatga tttacacatt cctagtgtgg tatgaaatct attttgtaaa atgttaatac
    31321 aagaggaaga atcacctgaa ataccattca ggtgattctt aatgttttat aattttactt
    31381 gctaattctt atgtctaact cttaatggta tatcaagttt ttttgccagt tgtttagatt
    31441 cttctatttc ttctggagat atttcatcta ctactgaaaa agctacttct tttatatatt
    31501 ttttgcaatc tcttgcaaaa tctaacatat aatcaaatga tttttctcca aactttggtt
    31561 gagtaactct attataagct tctttatttg gggcattaag acttattgat actgcatcta
    31621 taacattttt taacagaata gctgtttttt tgttatgtat taaatcactt agaccatttg
    31681 tatttattct tatctttatg ctactttttt cttttataaa ctttgctatc tctatcaatt
    31741 catttattct cattaatggt tcgccatatc cacaaaatac gatttcatca tagtcgtcta
    31801 aattgtattt attgaaggct tcttttactt catctacact tggctcatgc tccaaccata
    31861 aactaccact atttgcaact tcatcttttt cgtttcttat acaaaaaaca caactacatg
    31921 gacatgtatt agttatattt acatagatac tattttcaat tgtatataag atattcataa
    31981 ttttccctcc ataaatttaa attcttaatt aaaataacgt tttttaaaat caagtttatc
    32041 aagtgccata cctaagatta taaaaacaat aaaacttcct attggctgta ataagccaag
    32101 taagctcatt acaaatgcac ttgtaaaact tccaataatt ataccttcag caaacatgta
    32161 taaaactaat cctactatac ctgctacaac cgaagcaact atatttctct tatttattat
    32221 cttgtcactt ttagaagtga aaaataatac aagtattggt tttatgatta tagttggtat
    32281 tacccagata gcagcacctg tagataaatc agctagacca gcaccaattg cagaagcagc
    32341 tatagcatag tttgttggca aaatagttgc agctagataa ataaaagcat ctcctatatg
    32401 aacatatcca ttgtttccac caactggaat atgtaatata tatgctattg tcaggcatat
    32461 tatggatgca aataatgctg aagtaacaat ataggtagtt tttttagaag taactttatt
    32521 cattttttcc aatatactcc ctccctaaaa atccagagct aacataattg ttatgttttc
    32581 ttataaatat attaatatta atcatacaat tttcatagtt tgttaggtgt tttttttgta
    32641 aaatacagat tgtacccatt caaatgtata taattagaaa agtaaaaata ggtaaaatgt
    32701 atagagtagg agtagttaga ataagttaat ataaatttaa tttggtatga ataagaagag
    32761 tacaagttta atataaaaaa taatataaga gagtctactc aatatatact gagctgactc
    32821 tcttatattt gggaaattaa aggctatttt gttagataat agctaattaa tgaaaaaata
    32881 tatttataaa atactaattt actctattag tagcagttcc tgtttttata acttctacag
    32941 cactacttaa actagaataa actatatttt caatcgctgt actagtgaag aaaccaaggt
    33001 gatgagtgaa tataacttgt tccatagaaa gaagtttatt tatttctggg tcagttaatt
    33061 ctccaggatt catttttttg tttaagaaca atccttcagt ttcaaatgta tcaagggcag
    33121 cagctctaat ttttcctgac tctagtgctt ctattaaatc ccctgtatta attaaaccac
    33181 cacggccagt atttactatg taagctccat ccttcattat agctagagta tctttattta
    33241 tcatatgttt tgttccttca agtaaaggag tatgtaatgt tataaggtca gcttctttta
    33301 gaaggtcttc taaagaatct ttgtaagtta atatatcatt taaatcacta tttggatatt
    33361 ggtcaaatgc aattacatta gctcctaggc ctttgaataa ttttgcagaa gtagcaccta
    33421 tttttcctgt acctataaca ccaactgtaa tagatctaac ttctgttcca agaagttcag
    33481 ctatccaacg gtaatcacct tcacagactt ttttctttac tagaggagtc tttcttaaaa
    33541 ggttcatagc ttgagtaaca gctaattcag ctattgcatt aggtgaataa gaaggaacgt
    33601 ttgtaacgat aagtccattt tcattaacta aatcaaaatg tatcatgtca acccctgcag
    33661 ttcttgaagc tataacttta accccagcat cttttaaagt ttcataaata taaggattat
    33721 ctatatagtt agtttgttgt atagatatac catcataccc tttagcttta actacatttt
    33781 cttcagtcat gttttcagga taaatatcca cttgaacatc cttatgttct tcagaccatt
    33841 tttttattac tggttcttca taatcgcgtg ctccaaatac tagtattttc atatcaatac
    33901 cctcctgtaa ttattaaatt attatggtaa tttttcattt agaaataata tgtagcatat
    33961 aaggttatat tttttataca aaatttttaa ggttaaaaat ttagttttga aattgttttc
    34021 cctgtgttat tttgatattt agtaattttt tatttaatgt aataattact ctaaaattta
    34081 tcaaagaaat tgttattttt tagattaaat attattaaaa aattatctaa gtttgaaatt
    34141 agttaactaa cttgctgatt aattacaatg ataacatgtg tttttgagtt tagcaatgaa
    34201 aacttttttt gaaatgtcaa gctttttcat aaaaaaatat ataaaaaaaa cttttataag
    34261 catagaaaga atagcaaaat gcaatggata gtggcaagat gccacgatat gaaaaagatt
    34321 attcaattgc atattgttaa aaaacattca aatcaagcga tattttgatt aaatataaag
    34381 ttggcacgat aaatgcttaa caatataggt gtaagacaat aaaacaataa aaaataattt
    34441 taataatttt taagttattt tagataagat taggaacttt gacatcagac agttaatatc
    34501 agaaaaaagt attttatgag tgatttaatc gaaattttat aattgatact attaaggagg
    34561 aaaacgttat gcttttagaa ggagttaaag tagtagaact ttcaagtttc atcgcagcac
    34621 catgttgtgc aaaaatgtta ggtgactggg gtgcagaggt tattaagatt gaacctatag
    34681 aaggtgatgg aataagagtt atgggtggaa catttaaatc tccagcatca gatgatgaaa
    34741 accctatgtt tgaattagaa aatggaaata aaaagggtgt aagtattaat gtaaaatcaa
    34801 aagaaggagt agaaatatta cataaattat tatcagaagc agacatattt gtaactaatg
    34861 ttagagttca agcattagaa aaaatgggta tagcttatga ccaaataaaa gataagtatc
    34921 caggattaat attctctcaa atattaggat atggtgaaaa aggaccttta aaagataaac
    34981 caggatttga ctatactgca tacttcgcaa gaggaggagt tagccaatct gttatggaaa
    35041 aaggaacatc tccagcaaat acagcagcag gatttggtga ccactatgca ggtctagcac
    35101 tagcagcagg aagtttagca gcattacata aaaaagctca aactggtaaa ggtgagagag
    35161 taacagtaag tcttttccat acagctatat atggaatggg aacaatgata acaacagcac
    35221 aatacggaaa tgaaatgcct ttatcaagag aaaatccaaa cagcccatta atgactacat
    35281 ataaatgtaa agatggaaga tggattcaat tagctttaat acaatacaac aagtggttag
    35341 gcaaattctg taaggttata aatagagaat atatattaga agatgataga tataataaca
    35401 tagattcaat ggttaatcat gttgaagatt tagttaagat agttggagaa gctatgttag
    35461 aaaaaacatt agacgagtgg tcagctttat tagaagaagc agacttacca tttgaaaaaa
    35521 ttcaaagctg tgaagattta ttagatgacg aacaagcttg ggcaaatgac ttcttattta
    35581 agaaaacata cgatagcgga aatacaggtg tcttagttaa tactccagtt atgtttagaa
    35641 atgaaggaat taaagaatat acaccagcac caaaagtagg tcaacatact gtagaagtat
    35701 taaaatcttt aggctacgat gaagagaaaa taaataactt taaagatagt aaagttgtaa
    35761 gatattaatt agtaattcta gattttttaa aaggagaatg ctaaagatgt acacaatggg
    35821 attagatata ggttcaactg catcaaaggg agtaatctta aagaatgggg aagatattgt
    35881 agcttctgaa acaatatcct ctggtactgg gactactgga ccatcaagag ttttagaaaa
    35941 attatatggc aagacaggtc ttgcaagaga agatattaaa aaagttgtag ttacaggata
    36001 tggaagaatg aactattcag atgctgataa gcaaataagt gaattaagct gtcatgctag
    36061 aggggtaaat ttcataattc cagagacaag aaccattatt gacataggtg gtcaagatgc
    36121 aaaggtatta aaattagata ataatggaag actattaaac tttcttatga atgacaaatg
    36181 tgctgcaggt acaggaagat ttttagatgt aatggcaaaa ataatagagg ttgatgtatc
    36241 tgaactcgga agtatatcta tgaattctca aaatgaagta tcaataagca gtacatgtac
    36301 agtatttgca gagtctgagg ttatatcaca tttatctgaa aatgcaaaaa ttgaagatat
    36361 agtggcaggt attcatactt cagtagcaaa gagagtttct agcctagtaa aaagaatagg
    36421 agtacaaaga aatgtagtta tggttggtgg agttgctaga aatagtggta ttgtaagagc
    36481 tatggcaaga gaaatcaaca cagaaattat tgtacctgat atacctcaat taactggtgc
    36541 tttaggagca gcgttatatg cttttgatga agcaaaagaa tcacaaaaag aagtgaaaaa
    36601 tatataaaaa gagggatgaa aatgtctgaa aaaaaagaag ctagagtagt aattaatgat
    36661 ttattagctg aacaatatgc aaatgcattt aaagctaaag aagaaggaag acctgtaggt
    36721 tggtcaacat cagtatttcc tcaagagtta gcagaagtat ttgacttaaa cgtattatat
    36781 ccagaaaacc aagcagctgg agtagcagct aaaaaaggtt ctttagaatt atgtgaaata
    36841 gctgaatcta aaggatattc tattgaccta tgtgcatatg caagaacaaa ttttggtctt
    36901 ttagaaaatg gtggatgtga agctttggat atgccagctc cagatttcct actttgctgt
    36961 aacaatatat gtaaccaagt tataaaatgg tatgaaaata tttcaagaga attagatata
    37021 cctttaataa tgattgatac aactttcaat aatgaagacg aagttactca atcaagaata
    37081 gattatatta aagctcaatt tgaagaagct ataaaacaac tagaaattat atcaggaaag
    37141 aaatttgacc ctaagaagtt tgaagaagta atgaaaatat cagctgaaaa cggaagacta
    37201 tggaagtatt ctatgagttt accagcagat tcttctcctt ctccaatgaa tggatttgac
    37261 ttatttactt acatggctgt aatagtttgt gctagaggta aaaaagaaac tacagaagca
    37321 tttaagttac ttatagaaga attagaggac aacatgaaaa ctggtaaatc ttctttcaga
    37381 ggggaagaaa aatacagaat aatgatggaa ggtatacctt gttggccata tataggatac
    37441 aagatgaaaa cattagctaa atttggagtt aacatgacag gtagtgttta cccacatgct
    37501 tgggcattac aatatgaagt taatgattta gatggaatgg cagtagcata tagtactatg
    37561 tttaacaatg taaacctaga ccgtatgaca aaatatagag ttgattcttt agtagagggt
    37621 aaatgtgatg gagcattcta tcatatgaac agaagctgta aacttatgag tttaatacaa
    37681 tatgaaatgc aaagaagagc agctgaagaa actggattac catatgctgg atttgatggt
    37741 gaccaagcag accctagagc tttcactaat gctcaatttg aaacaagaat tcaaggttta
    37801 gttgaagtaa tggaagaaag aaaaaaactt aatagaggtg agatataatg gaagctattt
    37861 tatctaaaat gaaagaagta gttgaaaatc caaatgcggc tgtaaaaaaa tataaaagtg
    37921 aaactggtaa aaaagctata ggttgtttcc cagtttattg cccagaagaa attatacatg
    37981 cagctggaat gcttccagtt ggtatatggg gagggcaaac agaattagat ttagctaaac
    38041 aatatttccc tgcatttgca tgttcaataa tgcaatcatg tttagaatat ggattaaaag
    38101 gtgcttatga tgaattatct ggagttatta taccaggtat gtgtgataca ctaatttgtt
    38161 taggacaaaa ctggaaatca gcagtacctc atataaaata tatatcatta gtacacccac
    38221 aaaatagaaa acttgaagct ggtgtaaaat acttaatcag tgagtacaaa ggcgtaaaaa
    38281 gagaacttga agaaatttgt ggatatgaaa tagaagaagc aaaaattcat gaaagtatag
    38341 aagtttacaa tgaacataga aaaactatga gagactttgt tgaagtagct tataaacatt
    38401 ctaatactat aaaaccatca ataagaagct tagtaattaa gagtgggttc tttatgagaa
    38461 aagaagaaca tactgagcta gtgaaagatt taatagcaaa attaaatgct atgccagaag
    38521 aagtctgttc tggaaagaaa gttttattaa caggtatatt agctgattct aaagatatat
    38581 tagacatttt agaagacaac aatatatcag ttgtagctga cgacttagca caagaaacaa
    38641 gacaattcag aacagatgta ccagcaggtg atgatgcgtt agaaagatta gcaagacaat
    38701 ggtcaaacat agaaggatgt tcattagctt atgaccctaa gaaaaaacgt gggtcactta
    38761 tagtagatga agttaaaaag aaagatatag atggtgttat cttctgtatg atgaaattct
    38821 gtgacccaga agaatacgat tatcctttag ttagaaaaga tatagaagat agtggaatac
    38881 ctactttata tgttgaaatc gaccaacaaa ctcagaataa tgaacaagcc agaactcgta
    38941 ttcaaacttt tgctgagatg atgagtttag cgtaagttca ttaaaataaa tttactataa
    39001 aatatgaaaa ttggaggaag ctatgttata taacaaagaa caagaacttt taagaaaagc
    39061 agtaagagat tttgttagta aagaattaga tactttacca gcagaaatgg ataaaacagg
    39121 tgtaatgcct aaggaattaa tcaaaaaatt agctgatgca aaatttataa gcagtaatat
    39181 cccagaagaa tatggtggtg ggggagcagg atatgtttct tacgctatag taatggaaga
    39241 aatagctaga agatgtgctt caacagctac ttttgttaca gctggttctt ctcttgcttc
    39301 attaccaata ctttacaatg gtactgaaga gcaaaaacaa aaatacttaa aaggtatagc
    39361 aacaggtgaa ttaataggag catttggttt aactgagcca ggagctggtt ctgatgcagg
    39421 tggacaacaa acaacagcag aattagttgg agaccactat atattaaatg gtagaaaaac
    39481 tttcataact aatggaccat tctgtgatgt agctatagta atagcagtta cagatagaag
    39541 taaaggactt agaggaacat cagcgtttat agtagaaagt aaatgggatg gtttctcaac
    39601 aggagctcat gaagataaga tgggtataag aggaactgaa acttctgact taatatttga
    39661 aaacgttaaa gttccaaaag aaaacttact tggaaaagaa ggtcaaggat ttaagatagc
    39721 aatgggtact ctagaagttg gtagaatagg tgttgctgcc ttagctctag gaatagctca
    39781 aggtgcttta gatgaagctg ttaaatatac aaaacaaaga gttcaatttg gtaagcctat
    39841 agctaaattc caaaatactc aatttactat agctgacatg gaaacaaaag tttgtgcagc
    39901 tagaggatta gtttatgatg cagcacaaaa gagagatgca ggaatgagag ttgctcaaga
    39961 atctgctatg gctaaatact atgcatcaga aattgcaaat gaagttgctt ataaagcatt
    40021 acaacttcac ggtggatatg gatttataaa agattatgaa atcgaaagaa tgtacagaga
    40081 tgctagaatc gtatcaatat acgaaggaac atcagaagtt caaaaaatgg taatttcatc
    40141 aaacgtatta aaataataaa taatctaatg aaattggagg tatagacttt gaaaatatta
    40201 gtttgcgtaa aacaagttcc agatactaac gaagtaagaa taaataaaga aacaggtact
    40261 cttataagag atggagttcc aagtatatta aacccagatg atgcaaatgc attagaggaa
    40321 gctttaaaaa ttaaagatga gcatgatgat gtaactataa cagttataac aatgggacct
    40381 cctcaagcag actttatgct aagagagtgt ttagctatgg gtgctgatga tgcaatatta
    40441 ttaagtgata gagcatttgg aggagctgat acttgggcta catcaaatac tattgcagcg
    40501 ggaatatcta aagtaggtga ctatgacata atatttgcag gaagacaagc aatagatgga
    40561 gatactgcac aagttggacc acaaattgca gagaaattag acattcctca agttacatat
    40621 gtacaagatt tcaaaataga aggaaaagat ataatagttc aaagacaatt agaagatgga
    40681 tacgaattaa taaaagtaag tacacctgta cttttaacag cagtaaaaga gttaaataca
    40741 cctagataca tgtctgttga taaaatagta aaagcatata aacatgatgt aaaagtttgg
    40801 acaattgatg atttagatgt aaataaagaa gaagttggtt taaaagcatc accaactaaa
    40861 gtatttagat catttactcc tgaaccaaga ggaaaaggag aaatcttaga aggtaaagct
    40921 gatgaaatag ctagtaaaat aataatcggt ctaaaacaaa agcacattat ataagttgga
    40981 ggagtacaaa ctatgaatga tataaaagat ttaagttctt ataaaaacgt atggatattt
    41041 gcagaacaaa gagaaggaaa aatagctcca gtagttatag aattattagg agaaggaaga
    41101 aaattagcta aagaagtaga tgcagaactt tgtgcaatat tattaggaaa agatgttgat
    41161 ggattagcta aagaattaat cacttttgga gctgacaaag tttatgttgc agatgatgct
    41221 cttttagaaa aatatacaac tgatgcatat acaaaagtaa taaaagatgc aatagatgaa
    41281 ataaaaccag aaataatgct tttcggagca actcatatag gtagagactt agcacctaga
    41341 atagcttcaa gagttggaac tggattaaca gctgactgta ctaagttaga aatagaccca
    41401 gaagataaga aaataaaaca aactcgtcca gcatttggtg gaaacataat ggctacaatc
    41461 atttgtccaa accatagacc tcaaatgtct acagttagac ctggtgttat ggataaagct
    41521 gaaaaagacg aaacaagaac tggtgaagtt atagcattag actacaaaat aactcaagat
    41581 gatataagaa ctactgtttt agaaacagtt aaaactaaga aagatttagt atctcttaca
    41641 gatgcaaatg ttatagtatc aggtggtcta ggattaggtg gaccagaagg atttgaaatg
    41701 cttaagaaat tagctgacaa attaggtgga gtagttggtt cttctcgtgc ggctgttgat
    41761 gctggatgga tagaccattc tcaccaagta ggtcaaacag gaactactgt taaaccaaac
    41821 ctatatatag cttgtggtat atcaggagca atacaacatt tagcaggtat gcaatcatca
    41881 gatttcataa ttgctataaa caaaaaccca gcagctccaa ttttagaaat cgctgactat
    41941 ggagtagttg gagacttaca tgaaatagtt ccaatgctta tagaaaaatt agatagcgtt
    42001 gatgatttat tagaagctat aaaagcttaa tagatacaaa ctatataaat attactgatt
    42061 gaataagtct tattagttag taacataatt attaaccctt tgataagtta ttgctagtgt
    42121 gtatttgtca gttattaaaa agtctgtttg aataaaattt tataatacaa gataatctgg
    42181 ataatcctta tatgtatatt gcttacaaat tgtgagttat attctgtaag gattatcttt
    42241 gtttttttaa ataaatctta aatttaatta aattgtatta aatagacaaa taattactaa
    42301 atatgtgata aaatacaatt aaaagcaaaa tgaaattttt aacaaactat aatcattaaa
    42361 attatatctt agttacgttt taatgaaata aactacttga aaagacgaat attataagga
    42421 gttgattata tgtgctgatg gtatttattg taagcaaaca ataaattttg tatgtagttc
    42481 aataaattac ttaatgaaag gtgcgaatat taatggataa taatttaata gggcttatag
    42541 aacatgtaga aaatccagcc attttatgta aagaatcagg cgaaataata tactgtaatc
    42601 atctaataga tagtattttt agttttctag atataaaaaa gcctagaaat ataaatgaat
    42661 tagattcaaa ttttgacaaa acagaaatac tgacagatag taaaaagaaa atagctttca
    42721 gagagttaag gatgactgca catatataca atatgaaaga taataataat gaaaataata
    42781 tagtttattt atttgagaag tctctaatat ctgataaagt aattgaagat ataatagagc
    42841 atatagatga agttgttgtt gtgtttaaca aagatggggt aattgaaaag atgaacactg
    42901 tcagtgatga gatattacct tttaaaagaa ctgaggtact tggtagaaat ataacagacc
    42961 ttgtgagaca aggattagtt gaagaaccaa taatattaaa catgcttaaa gtgaagaaaa
    43021 agatttatag aaatatcgtt tacccagatg ggaagcttat tgcatatacg gcagttccaa
    43081 gatgggattc taagggaaag cttacaggag gagtactaac agggagagat atttcaaggg
    43141 ttattaagct tgaatctcag ataaaatata gtgatatatc agaagacaca gagtatataa
    43201 gtcaaagtaa gattatggac aatataaaaa aagttgttaa gagagctgca gcctcagatt
    43261 cttctatatt tataaatgga gaatcaggtg taggaaaaga aataatagca agaacaatat
    43321 ataagtatag ttcaaggaga gacaaacctt ttatagctat aaactgtgga gctataccaa
    43381 atgaattatt agagtcagag ttttttggat atgaagaagg ttcttttact ggagctaaga
    43441 aaaaaggtaa aaagggactt tttgaagagg cgaatggtgg aactattttc ttggatgaga
    43501 taggtgagtt accaatgcag atgcaaaaaa aattacttag ggttatacaa gaaaatacta
    43561 ttactagaat aggtggtagt aagcctataa aaatagatgt tagatatata agtgctacta
    43621 atatttctca tgaagacctt agaaataacc ttaaatttag acaagatttg tactatagat
    43681 taagtgttat tccagtaaaa atacctcctc ttagagaaag aaaagaggat atagtaccat
    43741 tagtaaatta tttcttaaaa ctttacaatg aaaagtacaa tagagaagta gaagtttcgc
    43801 ctaaagttat tgagttatta gaagaatatt cttggccagg aaacataaga gaattaaaaa
    43861 acatcattga aagatttgta gtattatcag ctaaaaatgt aattggagaa gatgaattta
    43921 acatgctgat aaatttagat atgattgata atgaaacaga tgatttatca ccaatagtgg
    43981 taaatggaat tatgaatctt aatgatgcat ataaaatagt ggaccaaata atgataagta
    44041 aggctataaa taagtatggt tcaataacaa aggctgctga agttataggt atatatcctt
    44101 ctaccatcca tagaaagata aagagtggac atattcacgt ttagctgtca gtttaaaaag
    44161 acaatataat taaaatatta taagatacat aatttataca acttaaaaga gatggcttaa
    44221 aaataaattt aattacttta agtcatctct tttcttttat aaatatttaa aagttatatt
    44281 agtttataga gttagcagaa cctaaaacat catctatctt gctttcaact acagcttgta
    44341 tagcatctct accagcacca atgtattttc ttgggtcaaa ttcttttgga ttttcagcta
    44401 agaattttct gatagcagca gtcatagcta gtcttaagtc agtatccatg tttattttac
    44461 aaacagccat agaagatgct tttcttaaca tatctacagg tacaccttta gcaccagcta
    44521 tatttccacc aaattcatta catgtagcaa cagcgttttg gtcaacagca gatgcaccat
    44581 gaagaactat tgggaaacct ggtaatttgc tttggatttc ttctagtata tcgaatctta
    44641 atttagcttc tcctttgaat ttgaaagcac catgagaagt tccaatagct attgctaatg
    44701 aatcaacacc tgttctttca acgaactcaa ctgcctcagc tggttgagta tacttgtgaa
    44761 catcagaagt aacatcatct tctgttccag ctagaacacc aagttctgct tcaacaacaa
    44821 cacctttaga gtgtgcatat tcaacagctt ctttagttat tctaacattt tcttcaaagt
    44881 caaaatgaga accatctatc ataactgaag agaaaccagc atcaatacaa gttttaatag
    44941 catccatatt tggaccatgg tctaagtgaa gagctacatc tacacctatt tcatcagaag
    45001 cagccttaac catttcaact aatgtatgag gtccagcata tttaacagct gacatagaag
    45061 cttgtatcat aacataagaa tttttagctt tagcagcttt taaaacacct tgtaattgtt
    45121 ctaagtcact tatgttaaat gcacctatag caaatccacc ttcgtaagct tttttaaaca
    45181 tttcttttgt agtaattaat gccattttaa actcctccta ttaactttta aaatttaccc
    45241 atcacttata ttataacgtt aaatcaaaaa aagtatatag taaattgtaa taactataaa
    45301 ttacaagtat aatgttaaat agaagacaat aggattaaat aaaataataa agaatataaa
    45361 caatttagga aaactagcta tataatgcta actataaggg taggaactac cttattagtt
    45421 tggagatact gataacaata gttgccttga ccaagaagtt accacttcaa acgttatcga
    45481 aaggaattaa gtggatgata attcactaaa aatttaaaat aaaggagata aactatgcca
    45541 cagataaaaa taagaggaat taatgaaaat gatatatgta aaataagtga aaaaatgata
    45601 aatgatttag ttgaagctgt aaaatgtcca agagactact ttgaaataga atgtataaag
    45661 tcagttgcaa taagagatgg taaaatagca gatgtatatc cttttgttga agtagcttgg
    45721 tttgatagag gacaagaagt acaagatata gtagcaagaa taataactga tagtataaga
    45781 aataatttag atgtagaaag tatagattta gcatttacag tatttgaaaa agaaaaatat
    45841 tatgagaatg gagagcattt ttaatggcta aaaagcaacg tatagataaa attctttcaa
    45901 atttaggtta tggaagtaga agtgaaataa agaagtattg taaacaaggg tctgtagtag
    45961 taaatggaag tgaagtttca aatccaggaa ctcaagtaga cactgaaaat gatgagatat
    46021 tatttaatgg agaagaagtt atatatagag aatatatata tttgatgatg aataaacctg
    46081 atggatatat atcagcaact acagataaat atgacccaac agtattagat ttaattgatt
    46141 tatcatactt agcttttgaa ccatttcctg ttggaaggtt agataaagac acagagggat
    46201 tattagtttt aactaatgat ggaaagcttt ctcatagagt tttatctcct aagaagcatg
    46261 tgcctaagac ttattatgca aaaattgatg gagtagttac tgaagaagat gtagaagctt
    46321 ttttagaagg tgtagtgctt gatgacggat ataaaactat gccatcacag ttaaatatat
    46381 taaaaagtga tgatgaatca gaaatagagc ttacaataca tgaaggtaag tttcaccaag
    46441 taaaaagaat gtttgaaagc gtaggcaaaa aagtagtata cttaaaaaga ttgtctatgg
    46501 gcaatttaaa attggatgaa agtttagagt taggtgagta tagagagtta acagatgaag
    46561 aagtaaaatt gattgaagaa agataataaa gatactatag taaattggag gaatgtctgt
    46621 gaagaaaaaa gatgttatag aatttgaagt agataaaatg gaatttggtg gtacatcttt
    46681 aagccaagtt ggagatagag ttatccatat gaagggtgga ataagtggtc aaaaggtgag
    46741 agctggagtt aaaaaagtta gaagtaagaa agctgaagtt aagatgatag aattattaga
    46801 gaactcacca cttgaaactg aagaaacttg caagcacttt agagagtgtg gaggatgtac
    46861 actattgtct gtgccatatg aaaaacaatt agaaataaaa gaaaaacaag ttatggattt
    46921 atttttaaag caagatttat ttggatttca gtttttaggg atagaaggaa gtccagaaaa
    46981 caaatattat agaaataaga tggaatatac ttttggagat gaagtaaaaa atggaccttt
    47041 gacattaggt cttcataaaa aaggaaagca tatagatata caaactgttg aggagtgtat
    47101 gttggttgat gaagacttct caaagatatt agtttctagt gtagagtttt tcaatgaaaa
    47161 aaagcttcct tattatagaa caatgaatca caaaggatat ttgagacatc ttgtagttag
    47221 aaaagggatt catactaatg agattatggt taatatagta acttcttctc aagaagattt
    47281 tgatatgtat gagtttaaag atatgctttt agggattgat ttaaaagctg agttagtagg
    47341 tgtacttcat actattaatg atggtttagc agatgcagtc caatgtgatg agttaagagt
    47401 attgtatgga agagattata tacaagaaga aattttaggt cttaagttta agatttcacc
    47461 attttcgttt ttccaaacca atactaaggg agcagaggtt ctttatagta ttgctaggga
    47521 ttttattgga gattacaatg ataaagttgt atttgacttg tatagtggta ctgggtctat
    47581 tggacaggtt atggctggtg ctgctaagaa agtgtatggt attgagatag ttgaggaagc
    47641 tgttgttgct gctaatgaga atgctaagtt gaatggactt actaattgtg agtttattgc
    47701 tggggatgtg gctaaggttg ttaaggattt aaaggacaag cctgatttga ttattgttga
    47761 ccctcctaga ccaggcattc ataaggatgc tattagggat atttgtggat ttggtgctaa
    47821 ggagattgtt tatatttctt gtaatcctaa gtctttggtt gttgatttgg ttgattttaa
    47881 ggggtatggt tatgagatta agatggttaa gtgtatggat atgttcccaa atacgccaca
    47941 ttgtgagact gtagtgttgc tacgtcgaaa ataatgctag aaatcttgag ctttagacag
    48001 tttcatagac atataatatt tagattagag ggtaataaaa ttaggaataa agaattgaaa
    48061 aggtgttttg atgtgtctgt agttatatat atggagtgtg ggaatacgaa ataaaaaaag
    48121 ttttaattat agaaatagat attaaattta taaatttaat atctattttt tatgtgaaaa
    48181 tatatttaat ctaaagaatg atatttcata aaaaaaatta aatatatttt taaaatattt
    48241 acaatagagt tattttttat tcgtataata atagaaatat tttagcataa gatgtataat
    48301 aaaggaaaaa atacagaatt aaaaaacata agatagataa gtggacttag gggagagaaa
    48361 ttattatgca attgacaaaa ataattctta gtaactatag gtgttttgga gaagaaccta
    48421 caactattga aattaataat ttaactggat ttataggtca taatagcagt ggtaaaactg
    48481 ctttattaag tgcacttatg aaactttttg gagaaaagtc tagtgataga attattgaaa
    48541 gatcagattt ccatatacct gaaagtattg atccagatag tatttctaag aatgaattat
    48601 atattgaagc acaatttgag tttcctgaat tggagagagg taaagacagc atatctatac
    48661 cagtattttt taaaaatatg gttgttagtg aaccaggtgg gaaaccttat ttaagaatta
    48721 gacttgaagc aaattgggag aaaagctcaa atcctgaagg aagtattgat tcaaatgtat
    48781 actatataac atctacaaca gaggagataa atcctgaaga taagcgtaat atcaaaagat
    48841 atgagcttga tcaaataaag atgatttatg tacctgccat taggagacca gatgaacagt
    48901 taaaaaatgt ctcaggaaca ataatttcaa gattattaaa tggtataagt tggtcagaac
    48961 aaacaaaggc aagaattaaa acgaaagcaa gtgaaacgga ggaaattttc tttgaagaaa
    49021 aaggggttga atcattaaag aaatcactcg aaactcaatg gaatagttat cataaggata
    49081 ttagatattc taatccaaag ataaacttca atagtactga tttagagaca attttaaaaa
    49141 aagttgaagt aactttttca cctacaggga cacatcgaga ttatcgggtt gaagagcttg
    49201 gagatgggtt acgttcttta ttttatcttt cacttgtaaa tacattttta gaaattgaag
    49261 aagaaattct aaaatctatt aatgatggta aagaagaaga tatatttaca ttaataccgc
    49321 ctgtattaac aattgttgcg gttgaagaac cagaaaatca tatttcacct catcttttag
    49381 gaagggtagt tgaaaaatta aattttattt ctacaaaaga aaattcacag acaatattga
    49441 catcacattc accagcaata gtaaaaagaa ttgaaccaac agacataagg tattttagga
    49501 tttgtaagga atatgaatgt acaaaagtga agtcaattat attaccaaat gaagaaaagg
    49561 aagcagaaca atttaaattt gtaaaggaat cagtattagc atatcctgaa ctttattttg
    49621 caaaacttgt tgtattgggc gaaggtgata gtgaagagat cattattaag cagatgattg
    49681 aattaaaagc agaatctgta gattcatcag aaatttcaat agtaccatta ggtggtaggc
    49741 atgttaatca tttttggcgt ttattaaatg atttagaaat tccatttatt actttgttag
    49801 atttagatag agaacgagat ggaggtggtt ggggacgtat aaagtatgca atacaacaat
    49861 taataaatat tggtcatgat aagaatgtat tactttatac atgtgaagga atttcacaag
    49921 ataatgaatt taaagagatg catacttggg atgtaactga tactgaaaat atggctaaat
    49981 ggattgaaat gctaaaaggt tatgatgttt atttttcagc tccgttagat atagattttg
    50041 caatgttaca aagttttaaa gataaatata tttcagcatt aacaagtgcg gaaggtccta
    50101 ggataaaaga tcatggaaaa atacaagata ttgtgatcaa tgaagatgct gaagatgaat
    50161 cattgaaagc tgcttataaa gatagaatag atagtgatat taaatcaacc ttaaaacaag
    50221 aaggtggtga tggatctact tatactgagg gagagaaaga actaatgata tggtattcat
    50281 atttcttttt aggaagaggt aaaccaacta ctcatttatc tttattttct aataatactg
    50341 atatagattt tgaaagtttt ccatactgtc taaaggagtt tgtaaatagt attattgaaa
    50401 agatgataga tgattcaggg ggtggaagaa aatgaagagg aattgggtta gtaaagaaga
    50461 ttggagtcca gcggctggat tcagactaga agttgaagcg atgaatacag ttattaatga
    50521 taagaataca cttgttgtag ctggtcctgg cgctggaaaa acagagttat tagctcagag
    50581 agcctgcttt ttactagaga ctaatacctg tagatatcct aaaaaaatcc ttgctattag
    50641 ttttaaaaag gatgctgctg aaaatttaaa agagcgttta gaattgagat gtgggaaaga
    50701 attagcttca agatttgaat caaagaccta tgatgctttt gcaaaagaaa tcgtagatag
    50761 atttagaaat agtttagatg aagcatatag acctttaaat aattacaata tagctgaaaa
    50821 tcgtgatgtt agaagagcat ttgagatagc aggattaaat ttcagaggaa atcaaactga
    50881 atttaataaa gtatatggaa ataaactatc gataaataaa ttgcctttac atacagattt
    50941 tgtagataag atttttatag atgcttggaa tgtactactt aaaggcaaca aagatactaa
    51001 ttttgtaagt tcacttactt ttggtatgat atcacgttta gccgaatatt tgataagaac
    51061 caataggtat attaagaaag caattgaaat gacttatagt catgtgtttt tagatgagtt
    51121 tcaagatact aaatctattc aatatgatct tgttaaagct tgttttttta aaacagaaac
    51181 aataattact gctgtagggg atggcaaaca acgaattatg ttatgggctg gtgctagaaa
    51241 gtcaatattt gaagattttc aaaatgattt tttaagtcaa aaaagaacat taattatgaa
    51301 tcatcgttca gcgccaagac ttgtagagat tcaaaaagca atgtataaca gtttgaatga
    51361 agaggcaatt gatttaaaaa ctaatgaaaa gtggaattat gaagatggag aagtgtattt
    51421 aagacttttt gaggattata taaaagaagc tgatatactt agtgaggaaa tagtcgaatt
    51481 ggtggaaaca ggtatagaaa ttaatgaaat atgtatatta gtaaaacaga gcgtagatat
    51541 ttattcaaaa gagattataa ataaactttc tgaacgtaat attaaggcgc gtaatgaagc
    51601 aatgtatcag gagatattaa aagaagatat aataaagaca ctggtttcgc tttttaggtt
    51661 gtcgttaagt gatagaaatc ccgatgattg ggattatatt tacgatattc taggtgaatt
    51721 atatggaata gacgaagggt ataaccctaa aatattaaat gatttcatta ccaaagtaga
    51781 attaatgatt aatgatctca aggtaaattt aaatattact gatgaaaaac aattttcaga
    51841 tttagttgat tctaaggttg aatatatttc atatgaaaaa tttgcatcaa agtttcaaaa
    51901 atataaatct aaagattatt ttgatgattt ggtagataaa tttaaaaagc tggtatggat
    51961 tgaatataat gaaactaaag attggattaa agcgattgat aactttgaag gtaaaaattc
    52021 aattccaata atgacaattc ataaaagtaa aggacttgaa tatgatacta tcttttttgt
    52081 tgggttagaa gattcagcat tttggaattt taaaaatcaa ccagatgaag atagatgtgc
    52141 attttttgtt gcattatcaa gagctaaaag aagaattgat tttacattta gcttagatag
    52201 accagcagga ttttcgaatt cacagaagca ttattgtatt aatgaattct acgaattgtt
    52261 tgataatact gagattgtaa aaaagataga gtattgatta agatgataaa aactttagta
    52321 tattatattt taagatatta gttatataag tagacattga atttatgtat ttgatgtcta
    52381 ttttttatat aaaaatatat taaagaaagg atgaatttgc atgaagaaaa aaacaaaaat
    52441 atctaaaaag attgtaagtt ttattatggt ttttataact atagttacta ctatacttac
    52501 atcatcagtt tcagcttatg ctagcgagtt ggatatgagt gaacagacag gctttaatta
    52561 tttaggtatt agtccaatta caggaaaaaa attaatcata acatttatcg tatgaagatg
    52621 gataataaaa ttgtattttg tgtagagcca ggaattaaaa cttcaagtgg aggaggatat
    52681 atatcggagg agttaggaga taaatttata tctacaacac ttccaaatta tcataaaaga
    52741 aaagctgaaa tacttgagaa ggtaaataga cataatatat taccttcatg gaataatgaa
    52801 aaagttgcag ttaaaagtgg agaaacaatt gagttagaag ataagaatgg tattattaga
    52861 gaaatgcaat tagaatcaaa taatactgga acagatatat tacttgaaga aaatactttg
    52921 aaaattacag caaataaaaa ttctaattca ggagttatta gttatataaa gattcctaaa
    52981 aatgaggttg gaatatcaat agtttatcgt aagcataact atcaaagctt atcagaattt
    53041 catatggaaa gttcgatgag tgcaaattta aatataaatg taatcaagtt aggaattgtt
    53101 aaagtaaaaa agattgatga aggcacagga aaggaattag caaatgcaaa acttagattt
    53161 gagtatgatg gtgatagtaa agaaattata acgggttcta atgaaattgc tagtatagaa
    53221 gatattattg gttgattaaa aagtgaagta tacttttgta tctatgaaaa agactataag
    53281 caatatataa aattagatat acctattgat gaagcaaaaa taaaaaatag atttgaaata
    53341 aggttaaaaa atgaaagagc atatcatgca gcagttgatt tgttagcaaa tagagatgaa
    53401 gaaaagacag cattttcaat tattaatcgt tatatacgtt ttgttgataa agatgatgaa
    53461 aaaattagaa gtgaatggaa aactaatgat cagtggagtt attttattgg tgaaggtaga
    53521 gcgaagttaa aactaacaac aaatccagaa ccatataata ttcaaaaaac tttaaattgg
    53581 ttatcaagac aagttgcacc aacgttaaaa acaattaaaa ggttagatag tttgaacaac
    53641 tcaacattaa ttgagggctt aataaggtat gcaagactaa cagataaaca taaaaaaata
    53701 attgaacaaa attcagtaaa tgttgaagac attatttatg atgaataaaa aaatgtaatt
    53761 agtattgcag attttaatat aaaagaaggg agatatacga atgaattttg gtcaaaattt
    53821 atataattag tttttatcaa atgcacaatc attggtatta atggctatag ctataattgg
    53881 tgtatattta ggattttaga gagagttttc aaaacttata ggatttttag tagttgcatt
    53941 aattgcagta gggcttgtgt ttaatacagc aggagttaaa gatatattat tggagctatt
    54001 taataaaata ataggtgtgt aaggattaaa agagaataga aaattatgag tatatgatga
    54061 agggttctaa taagttttat taaaatatat attgaaagac aagtagagct atttcgagtt
    54121 attacatgta atgctaatat atataactgc attttggaaa ttaaatacat aattagaaat
    54181 agcaaatttt ataggtgact tattaatatg tttacttatt gtatactatt tgatatattt
    54241 aatatgaggt gattcttgaa aatggacata gtgaaagttt ttgctaacaa tgtgagaaaa
    54301 tatagaaata aaaaaggtct atcacaagaa gcttttgctg aaaaagctgg attgcataga
    54361 acttatattt gtgctattga aagaaaaaag agaagtattg ctttagataa tgtgcagaaa
    54421 attgctgatg cgcttgagat tgagacttac ttactattta tagaaggtga ggagaaagaa
    54481 tgtctatgaa aaaattacca atagaaaagc ttatagaaaa tttaggtttt tcagggtcaa
    54541 agaatcttgt gaatatagat atgcttacaa aattagatat atctaaacat gatgtaaaaa
    54601 ctttaaaagt tataaatcct tatgcaattt atttagtaaa tagtaagcca tttatattat
    54661 ttatagagtc ttcttttgaa gaaaaaaaat tacaacaaat tagtaagctt atatggaatg
    54721 ctcaaatacc aattgctttt atatgcgatt ctaattctgt taaaatattt aatggaaaag
    54781 ctttggattt aaagtcatat ttaattgagg aaatatataa tcaatctatt gaggattttt
    54841 cggaagaatc tgagttttct tatttgaaaa tatctgatcc attattttgg gatcaataca
    54901 ctaaacaata taccaaaacg catttaaatg attatttgct agataatatt acttcattga
    54961 caaataaatt aaagaaaaaa tataaggtga aatttgcaac gaagcttgtt ctaagattaa
    55021 tatttattag atatttaatc gatagaggtg tcgatatagc ttataaaaat tttaatcatg
    55081 atattaaagc ttcacaaaaa gaactgctaa agattactaa aaacaaagtt gaattatatg
    55141 aactgtttaa atacttaaaa gaaaagttta atggtaattt atttgagtta aatgacgaaa
    55201 ttaataatac ttgtttaaca gatgaagtgt ttattttact atctgatttt ttatcaggta
    55261 aaataagtat tgacaatgga caaatgtctt tattttcaat gtatgacttt aatatcatac
    55321 cagttgaact aattagtaat atatacgaaa ttcttttagg aaaagaaatt cgtaataaag
    55381 ataacgcatt ctatactccg aattacttag cagaatatat tttagataaa acaacacttg
    55441 aatttctgaa aaataatagg caatacaaaa ttctagatcc ttcttgtgga tcaggagtat
    55501 ttttagtaaa tagttttaga cgtattattg atatgaattt gggtgataaa cttttttgtg
    55561 aagatgataa acttttaaaa gaacttttat tatctaatat ttatggaatt gatatcaatg
    55621 aagatgctat cgatgttact attttctcgt tatatctaac tattttagat tacaaagacc
    55681 ctaagatatt atcagaattt aaactgccta atttaaaagg gtgtaattta atagcgagtg
    55741 atttttttga tgataataaa ttggagagtt taaagaaaat taactttgat tttatcattg
    55801 gaaatccgcc ttggggaagt gtctcagatg gattacattt agattattgc aataaaaatg
    55861 gctataatga tatgcaacaa aacaaagaaa tcagtagaag ttttattttt agagcaaaag
    55921 attttagctc tagaaataca gtatgttctt ttgtagtgca ttctaaaata ctatatactc
    55981 aaaaaaagcc atcaaaaaaa ttccgtgaat ttttgcttaa aaaaagtcaa atatttagtg
    56041 tagttgaaat gtcatctgta agaaaattag tgtttaaaaa tgcagacgct cctgcagcag
    56101 ttatatcatt taagtataac gaaagtgaag aaaatttaaa taatagaatc atctatacat
    56161 ctttaaaacc aaatgtattt tttaaattat ttaacattat tgtaattgaa aaatatgatg
    56221 ttaaatatat ttcacaaaaa ctattattgg agaatgattg ggcttggaaa actattgtgt
    56281 atggattcag taaagatata gatataatta tgaatatcaa aaaaaaatat caaactgtaa
    56341 atgaaattat tttgaaaaac agttttgttc atggaacagg gatacaaatc aatggtaatg
    56401 aaagccatga tgcgacacat ttaaaaggaa agtggtatgt tgattcaaag aaaggaatag
    56461 attcattcta tgttgatata aataaaggga aagaacttac tcaagataaa atgagaagac
    56521 ctaaggctaa tcagcaagaa atatttattc ctccatatgt attattaaaa aagggagttg
    56581 acacgaaaac atatagatat agagcagcat tttcaaatga tgcatttgta tattcagatg
    56641 cagttacagg aatatgtgga aaaaaagata atttaactac actttattgc ttgactggta
    56701 tgcttaactc ttctttttac tcatacttaa atataatgct aggtacatca attggaatag
    56761 aaagagaaca aggattttca acggaaattt ttacgtatcc agcaatagaa agtgatgaaa
    56821 ttgctaatct ggtagtaaaa atacaagaag cgactaaatt agaaaaaaca actatgttct
    56881 tgaatgaaaa aaatagttgt aagtatatca aacaattaga taagttaatt ttgaagtctt
    56941 ttaatttaaa tgatgataaa tttattgact atgcaataaa tattcaaatt cctgagctta
    57001 caaattctaa gtcaactaac atatatagaa aaataactaa ggaagattta attaattata
    57061 gtgaatgttt caaagaacag ttttcatcga tatatcgtag ggttgggaaa agtatttcaa
    57121 tttcggtata tccaaaaata ttaaatcgtt ttacagtatt tgaactagaa gtaacagatg
    57181 aaaaaacaga tagagagatt atggttaaag cagaagctga taataatatg gagttgttta
    57241 cgaaaataat tagatatgat tataatgatg tttttcatca aattagagat attattcatt
    57301 ttggagaaaa ttcattttat atcataaagc ctaattgcta taaatattgg catcctgcta
    57361 tcgcagaaat ggatcttgct gatgttttgg atcagatttt atctgataca gggggtgaag
    57421 aataatggga ggatttaaaa attcttcact aaacttttat ttaaagcaag ggctttatga
    57481 aataattata gatggattaa tagactgttg tttacttatg agcaaatcat gtattgaaca
    57541 atctagaaaa atattaaatc acgaagaaaa gataagaacg catttagttg aaaattattt
    57601 agaaaatgat attgaaagaa gaaaaacatc tttatctaaa caacatctaa gatttgaagt
    57661 agaagcacaa gaaaattatg acaacaaaaa tgaatcttat gagggtaggg ttgatataaa
    57721 agttatatct aaagacactt tagcggatag aagggcttat tatatcattg aatgtaaaag
    57781 gatagatggt aaggaaactt taaataaaaa atatataacc gaaggtgtac aacgatttat
    57841 tactgagaaa tattcttcgc attatggcaa aaatataatg cttggattta tagtaaaaga
    57901 tataaatatc cagagcaata tacaaaaaat taattcactg aatcagaaca tattatatac
    57961 atatatagat acagatatgt tgacagttag aagtgattct gaaaataatt atgctgtttg
    58021 tgaaagtaaa tataaagtaa aatctaaact actattgtta aatcatattt tttataactt
    58081 ttcgagtata ataagataat cttatgaata taaaatatta ctatgttaaa attatagttt
    58141 ggttttgata atattataaa aataagttta agaataaaat ataaaggaaa aaaggggcgt
    58201 agcactattt tttagtgtga cagccccttt aattttaaaa tcggtaagga ggagtgtatt
    58261 tgaaaaaaat taagtcatat acagggattt tgaatgtgga gaaggttata tatgcaataa
    58321 atgattttca gcttccattt ccagttactt ttacgcagat gacatggttt gtattaacac
    58381 tttttataat tatattattt ggtaagttac ctccattatt tatgatagaa ggagcatttt
    58441 taaagtatct tggcatacca atttttataa cttagtttat gtctcaaaag acttttgatg
    58501 gaaagaaacc atatggattt ttaaagtcaa taatactata ttcattaaga cctaaaataa
    58561 catacgcagg aaaaaacatt aaatttaaaa ataaaattat tgatgaaaat ataactatag
    58621 taaggagtga attatatgtt cccgataaaa tatatagaga acaatcttgt atttaataag
    58681 gaaggagaat gttttgcata ttatgaatta ataccatata attattcatt cttataagct
    58741 gaacaaaaat ttattgtaca tgataatttt aggcaattaa tatcacaaag tagaaatggt
    58801 aaaattcatg cacttcaaat agccactgaa agttctataa gagttacaca agaaaggtta
    58861 aagaaaaata ttacaggaaa attaaaagaa attgcttatg aaaaaataga cgagcaaaca
    58921 gaggcacttg tttctatgat tggcgataat caaattgatt atcgtttttt tattgggttt
    58981 aagcttttag ttacagagaa agaaatgaat ataaagagta taaaaaagtc aatattagct
    59041 aatctatcag attttatata tgatgttaat aataaattga tggatgactt tgtcagtata
    59101 aataatgatg aaattatgag atttttaaaa atggaaaagt tattagaaaa taaaatctca
    59161 agaagattta aagttagaag gttagataaa aatgattttg gatatttaat tgaacataat
    59221 tatggaaaga aaggcacagt ttttgaagat tatcaatacc atttacctac aaagaaactt
    59281 aaaagagaaa cattagtaaa gaaatacaat cttataaagc caactagatg tcttatagaa
    59341 gagaagcaaa gatacttaaa aattatacat gaggattcag aaagttttgt agcttatttt
    59401 actattgaaa ttattattgg agaaatgaat tttccatcta gtgagatatt ctattatcag
    59461 caacaacagt ttgcatttcc tattgatact agtatgaatg tggaaattgt aacaaataaa
    59521 aaggctttaa ctacagttag aaataagaaa aaagaattaa aagatttaga caatcatgct
    59581 tggcagtcag ggaatgaaac agtaacaaat gtagtagaag cattagaggc tgtaaatgag
    59641 cttgtaaata atttaaatca aacaaaagaa tcaatgtata agttaagcta tgtaattagg
    59701 gtatcagcag atagcattga tgaattaaaa cgccgttgtg atgaagttaa agatttttat
    59761 gacgatttaa atataaaatt agtacgccta atgggagata tgataggatt tcacaatgaa
    59821 tttatacctt caagcaaaag gtatataaat gattatattc aatatgtaac tagtgatttt
    59881 ctagcaggat tagggtttgg agcaactcaa tagcttggag agaacgaagg aatatatatt
    59941 ggatatagtt tagatacagg aagaaatgtc tatcttcaac catctcttgc aagtcaagga
    60001 gtaaaagaat ctgtaactaa tgcgttggta gcagcattct ttggattttt aggaggtggg
    60061 aaatcatttg ctaataactt acttgtttac tattcagtat tgtttggagg gcaagctgtt
    60121 ttggtggatc caaaagctga acgtggcaaa tggaaagaaa atttaccaga tatatatcat
    60181 gaagtaaata ttgttaattt aacaagtgat atagaaaata agggaatgct agatccttat
    60241 gtaattatga aagaagtaaa agatagtgga agtctagcta ttgatatatt aacttttctt
    60301 acaggaataa gtagtcatga tggagagcag tttcctatac ttagaaaatc aatcataaat
    60361 gttactcaag acaaatgcag aggacttttg aaggtaatag atgaattaag gaaagataat
    60421 tctcaaatat caaataatat tgcagatcat attgaaagtt ttacagatta tgattttgca
    60481 caattacttt tctcagatgg aaatattaaa ggctcaatta atctagaaaa acaaatgaat
    60541 ataatacaag ttgcagatct tgttttaccg aataaagata ctttatttga agaatataca
    60601 acaatggaaa gtctttcagt agctatgttg atagtaatta gtacttttgc attagatttt
    60661 atccatacag atagaagtac ttttaaaata gtagatttag atgaagcttg gacatttctt
    60721 aatgtagcac aaggaaaaac tttatctaat aaattagtta gagcaggatg tgctatgaat
    60781 gcaggagtgt attttgtaac tcaaaatgca ggtgatgtat cagatgaaaa tttaaaaaac
    60841 aatattggat taaaatttgc attcagaagc acagatatca atgaaattaa gagaatactt
    60901 gaattttttg gagttgataa ggaagatgag aataatcaaa aaaggttaag agagttagag
    60961 aatggacagt gtttaataca agatttatat ggacgagttg gggtaataca aatacatcta
    61021 gtttttcaag aactccttac tgcatttgat acaagaccac ctataagaaa agaggtggat
    61081 taatgaaatc gaaagtattt agaaatttaa gctggataac tattttaata gtagtacttt
    61141 tactattttt tatttatata aatttataga tttagagaaa caaatagata cattagataa
    61201 gaaaagaaaa aaactcatag atatgagatt agaagatata attgataaag aatcatatga
    61261 agaaaagtat gatgatttaa cagttgaaat aaagaaactt agtaaagaga aaaaaactct
    61321 taaagcatca ttaataaatg aaaagagtat aaaagcaaga cttagagaat ttagaaagat
    61381 acttgaaaga aatgaattgt tagaaaaatt tgatagatat gtatttgcaa gtatagtaga
    61441 taaggttata gttggagtta ttaatgaaga tggtatagtt gatccttata aaataacttt
    61501 tgtttataaa actagctttg atgataaaca agatggacat aaatttataa ataagagtcc
    61561 gttagaaacg tattcaaaag ataacaacga taataaaaag aagtgttcat atactaataa
    61621 tgagacattg cgagactgta gttaaactta ttagagaata acaaggtttc agacttttat
    61681 attgtgtctt aaaagtagat ttgccaccta tttgccacca taattatttt tgggtggcaa
    61741 atttattcta caatgatttc aaaaatattt accgtctggt ttctcatgtt gttagttaca
    61801 tgtaaatatg tatccatagt tgttgaagtt ttagcatgtc cgagtatatg ctggatatct
    61861 tttatattag taccattttc taataacata gtatcagggt ttttaaaata aagtgtgtat
    61921 tcttctaaag tagtgataat taaaatatca aatatcacta tacaaaggaa agtgcacgct
    61981 ttttatgttt aacaaaaacg aaatattaaa aaacttattg aagaatatga tgtcaaaaca
    62041 actacagata ttcaagatat gctaaaagat ttatttacaa atacaattca tcaaatattt
    62101 acattgtcga attttagcgg tttaaacata atatattgac tgatataata aaatcataga
    62161 atattttagg gggaaataga ttatgtttaa gaaaaagtta atagctgttg caatgagtat
    62221 aactatgatt agtgtaggga gcttttcgta tgctcattca ggaagaactg attcatcagg
    62281 aggtcatcgt gataataaaa ataaatctgg attaggaagt tatcactatc attgtggtgg
    62341 ttatccagct catttacata ataatggtgg ttgtccttat actggaggag gaagcagttc
    62401 tggaacaaca acttcggtta ataatgaaga aaagcaaaag aggtctgttg gagaaaaagg
    62461 atataatcaa gggtatgaag atgggtataa aggaaattat agttcatcaa attatagtgg
    62521 agattatagt gatacatatg aatcaaagta ctcagaagga tatgaaaaag gtaaggctaa
    62581 attagaagag gaagaaaagg tggctaaaga gactggatat aatttagggc ttacaggtgc
    62641 taaatctaat aatacatatg aaaaagaagt tttaaaaaat gcttatgata ccggatattc
    62701 aactggatac aatgaatata agactaaaaa gatagaagaa tataaagcta aagggataga
    62761 ggatagcaat aaagataagg aaaaaatgac atttgaagaa aacatagatt ctgaatttat
    62821 agatgcttat aataatgctt atgacgaaat acaagaacaa ttaaaaaatg attacactac
    62881 tcaaggattt gagagtgcta tcaaaggaga aagatttgac acatcaacta taggtaatgt
    62941 taaatatgct aattggttta aagaaggata tgataatggt aaggttaagt tacctaaagc
    63001 aaaagaaagt gtatataatc aaggttacaa tgaagaagat tttagtatac cagatgaaat
    63061 gaagtctatt gaaactaagt taaaaggtgt atatgatgaa ggtttagaaa aaagagaaga
    63121 agagaaaagt agaaatgcta cttatggggt tggagctgga acagcagctg tagttgctgg
    63181 agtagtatat aagaagaaaa aacgtaaaaa gatatagatg ctagatatac caatgatgac
    63241 aagtgtataa caaatataat tttaagtata aatatttaaa atatagaagg gaaggttata
    63301 tctatgggat ttaactttag gaaaagcata aatctaggag gaggactcaa attaaattta
    63361 ggtaagaaga gtgtaggtat aagtgctggt gttaaaggtg ctagagtgtc agttaattca
    63421 aaaggaagaa aatcagctac attgagtata cctggaactg gaatatcata tacaaaaact
    63481 agtacaaaga aacgtaaacc taaataaata tttgatatac taagaaatag actgttacaa
    63541 atagtctatt ttttagtata tagatttaaa aagtgacaaa attttgaaat ataaactaat
    63601 ttcataaagg aaaatattat tttatgtaga attataatat ttaagtaata ttataaaatt
    63661 tataggagga tttcattatg aaattaagca aaatactatt ggcaataggg atgtctgttt
    63721 cattattgat tgctagttgt ccaacatcaa acgctcttag ttctattaat tcaataaaag
    63781 gtacaaataa gtatgaaaca gcaggtttaa tagcagataa acaaaattat gatattgcaa
    63841 tacttattaa ttctgaaaat ggattatctg atggattgtc agcaagtgga ttgtcaggag
    63901 ctaaaaatgc cccaatattg ttaactaaga aagatacaat cccttcagaa acattaaaaa
    63961 gattagaaaa ggtaaaagaa gtatatctaa ttggaggaaa aaattcgata ggagcatcag
    64021 ttcagaaaat attaagtgat aaaaaaataa gcataaatag aataagtgga actgatagaa
    64081 tagatacaag tcataaagtt gcaaaagaaa taggaagagt aagtacggtt aaaaaagtaa
    64141 tattaactaa tgcttttaaa ggcgaaccag atgctatgag tgttgcttct atagcagtta
    64201 gagataaatc tccaatagta ttaactgatg gaaatactac aagttttgct acagatggat
    64261 tagaaactta tgcaattggt ggaaaagtca gtatgagtga taagttaata aatgaaacta
    64321 aagcaacaag aataggtgga gtagatagat acgatacaaa taaacaaata ataaataaat
    64381 tttataatgg ctcaaaagaa ttttacatag caagtgggac tgatttagtt tattctttag
    64441 ttggttctac aattgcacaa aaaacaccta tagtgttagt tggaaatgga agtaataaga
    64501 gtatattaaa aaatgcaagt aaaataactg ctattggaaa tattgatgat gttgttataa
    64561 aacaatgttt aaatataact aataatgttg gggattcaaa tacaggtatt gtaaaagata
    64621 ctaattcaaa taatacgaac aaaccaagta atgagcctag ctctaataat cctggattaa
    64681 ctgataaaag tacagtatat gtaactccta atggaaaatc atatcatcta acaaaaaatt
    64741 gttctacact aaaaagaagt aaaacaataa ataaagtaac attaaaacaa gctaagtcac
    64801 aaggtaaaat agatccttgt aatgtttgtg taaaataatg tattagccaa atatatatta
    64861 aaaaagacaa ttaattaaga ttgtcttttt tattgaagaa atataaattt atattatgta
    64921 ttattaaaaa aaaattatta attataaaaa ataataataa agacgataag tattaaaatt
    64981 ttatgtttcc aatatttatg atataattaa tatggcaagg aataaatatt tgaaaaagct
    65041 atgagtggtg tttccatgga agtttcttca ctttcttatg agaggaggtg aaaactatgg
    65101 aaaatctaat gatgagtatt ataactggtg ttatagccag ttacatttac gataaattaa
    65161 agagccactc tgagcgacca actaagagtg gctgggaact taatataaag ttccagaaaa
    65221 gtaaatcttc aaaataattt gttgaaggaa acatcactca aagttaagta aaatatgttt
    65281 ccttgctttt attatatcac aaattaaaaa tattaacaca aaattttgtg aatatatact
    65341 tattattgtt ctaaaaaaat atgtaaataa ttgatttgat tgcatggaat tattgatgta
    65401 atatttaagt taaatataca caatctcaat taaagaagat aagtactata aattcatatc
    65461 aaaaaaatac ataaactttt aagttataat tttataaaaa gtcttttagt atcattattt
    65521 caagaggaat tatcatatga aaaaataact tcattagaaa agtggataaa aaactggtaa
    65581 ga
//
LOCUS       DAYAOY010000008        65253 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYAOY010000008 DAYAOY010000000
VERSION     DAYAOY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.008"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            107..1264
                     /locus_tag="ACGI6B_000581"
     CDS             107..1264
                     /locus_tag="ACGI6B_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9750047.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(1411..2046)
                     /gene="lexA"
                     /locus_tag="ACGI6B_000582"
     CDS             complement(1411..2046)
                     /gene="lexA"
                     /locus_tag="ACGI6B_000582"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFG9750048.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(2150..3277)
                     /locus_tag="ACGI6B_000583"
     CDS             complement(2150..3277)
                     /locus_tag="ACGI6B_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9750049.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(3588..4433)
                     /locus_tag="ACGI6B_000584"
     CDS             complement(3588..4433)
                     /locus_tag="ACGI6B_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750050.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(4463..4834)
                     /locus_tag="ACGI6B_000585"
     CDS             complement(4463..4834)
                     /locus_tag="ACGI6B_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9750051.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(4987..5247)
                     /locus_tag="ACGI6B_000586"
     CDS             complement(4987..5247)
                     /locus_tag="ACGI6B_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HFG9750052.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(5436..6380)
                     /locus_tag="ACGI6B_000587"
     CDS             complement(5436..6380)
                     /locus_tag="ACGI6B_000587"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFG9750053.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(6401..7255)
                     /gene="accD"
                     /locus_tag="ACGI6B_000588"
     CDS             complement(6401..7255)
                     /gene="accD"
                     /locus_tag="ACGI6B_000588"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFG9750054.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(7270..8631)
                     /gene="accC"
                     /locus_tag="ACGI6B_000589"
     CDS             complement(7270..8631)
                     /gene="accC"
                     /locus_tag="ACGI6B_000589"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFG9750055.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(8647..9099)
                     /gene="accB"
                     /locus_tag="ACGI6B_000590"
     CDS             complement(8647..9099)
                     /gene="accB"
                     /locus_tag="ACGI6B_000590"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFG9750056.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(9369..9815)
                     /locus_tag="ACGI6B_000591"
     CDS             complement(9369..9815)
                     /locus_tag="ACGI6B_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750057.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            10090..10368
                     /locus_tag="ACGI6B_000592"
     CDS             10090..10368
                     /locus_tag="ACGI6B_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750058.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(10497..11228)
                     /locus_tag="ACGI6B_000593"
     CDS             complement(10497..11228)
                     /locus_tag="ACGI6B_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750059.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGKA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(11372..12016)
                     /gene="trmB"
                     /locus_tag="ACGI6B_000594"
     CDS             complement(11372..12016)
                     /gene="trmB"
                     /locus_tag="ACGI6B_000594"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFG9750060.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(12154..12474)
                     /locus_tag="ACGI6B_000595"
     CDS             complement(12154..12474)
                     /locus_tag="ACGI6B_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HFG9750061.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(12927..13175)
                     /locus_tag="ACGI6B_000596"
     CDS             complement(12927..13175)
                     /locus_tag="ACGI6B_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9750062.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(13206..13667)
                     /locus_tag="ACGI6B_000597"
     CDS             complement(13206..13667)
                     /locus_tag="ACGI6B_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9750063.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            complement(13705..14538)
                     /locus_tag="ACGI6B_000598"
     CDS             complement(13705..14538)
                     /locus_tag="ACGI6B_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750064.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(14812..15498)
                     /locus_tag="ACGI6B_000599"
     CDS             complement(14812..15498)
                     /locus_tag="ACGI6B_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750065.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(15513..17990)
                     /locus_tag="ACGI6B_000600"
     CDS             complement(15513..17990)
                     /locus_tag="ACGI6B_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9750066.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(18209..19561)
                     /locus_tag="ACGI6B_000601"
     CDS             complement(18209..19561)
                     /locus_tag="ACGI6B_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9750067.1"
                     /translation="MIFICLILTVIGIFLSLITIQKPNGEFIRSDWPKKFTENFNEQI
                     VFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPKEHKDYYSNSELLALANQ
                     NNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGKFIGGKSIVFSIIGIIFL
                     IILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHKS
                     DEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSSTNYSPEKVYKYASIMLKN
                     IEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSIDILNYPEYESNIVEFKSN
                     IDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLNERIEIIISDNGKGMSRE
                     EASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI
                     "
     gene            20111..20971
                     /locus_tag="ACGI6B_000602"
     CDS             20111..20971
                     /locus_tag="ACGI6B_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9750068.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(21210..21767)
                     /locus_tag="ACGI6B_000603"
     CDS             complement(21210..21767)
                     /locus_tag="ACGI6B_000603"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9750069.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(21957..22844)
                     /locus_tag="ACGI6B_000604"
     CDS             complement(21957..22844)
                     /locus_tag="ACGI6B_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9750070.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            22963..24162
                     /locus_tag="ACGI6B_000605"
     CDS             22963..24162
                     /locus_tag="ACGI6B_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HFG9750071.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(24356..26911)
                     /locus_tag="ACGI6B_000606"
     CDS             complement(24356..26911)
                     /locus_tag="ACGI6B_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9750072.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(26908..27594)
                     /locus_tag="ACGI6B_000607"
     CDS             complement(26908..27594)
                     /locus_tag="ACGI6B_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750073.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(27671..28579)
                     /locus_tag="ACGI6B_000608"
     CDS             complement(27671..28579)
                     /locus_tag="ACGI6B_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9750074.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(28581..29252)
                     /locus_tag="ACGI6B_000609"
     CDS             complement(28581..29252)
                     /locus_tag="ACGI6B_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750075.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            29514..30578
                     /locus_tag="ACGI6B_000610"
     CDS             29514..30578
                     /locus_tag="ACGI6B_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HFG9750076.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            30736..31422
                     /locus_tag="ACGI6B_000611"
     CDS             30736..31422
                     /locus_tag="ACGI6B_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750077.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            31412..32443
                     /locus_tag="ACGI6B_000612"
     CDS             31412..32443
                     /locus_tag="ACGI6B_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9750078.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            32525..33190
                     /locus_tag="ACGI6B_000613"
     CDS             32525..33190
                     /locus_tag="ACGI6B_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750079.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            33203..35620
                     /locus_tag="ACGI6B_000614"
     CDS             33203..35620
                     /locus_tag="ACGI6B_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9750080.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(35801..36592)
                     /locus_tag="ACGI6B_000615"
     CDS             complement(35801..36592)
                     /locus_tag="ACGI6B_000615"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9750081.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(36625..37212)
                     /locus_tag="ACGI6B_000616"
     CDS             complement(36625..37212)
                     /locus_tag="ACGI6B_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9750082.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(37389..38780)
                     /locus_tag="ACGI6B_000617"
     CDS             complement(37389..38780)
                     /locus_tag="ACGI6B_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFG9750083.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(38964..40268)
                     /locus_tag="ACGI6B_000618"
     CDS             complement(38964..40268)
                     /locus_tag="ACGI6B_000618"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HFG9750084.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(40627..41502)
                     /locus_tag="ACGI6B_000619"
     CDS             complement(40627..41502)
                     /locus_tag="ACGI6B_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFG9750085.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(41706..42392)
                     /locus_tag="ACGI6B_000620"
     CDS             complement(41706..42392)
                     /locus_tag="ACGI6B_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFG9750086.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(42441..42974)
                     /locus_tag="ACGI6B_000621"
     CDS             complement(42441..42974)
                     /locus_tag="ACGI6B_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFG9750087.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(43018..43650)
                     /locus_tag="ACGI6B_000622"
     CDS             complement(43018..43650)
                     /locus_tag="ACGI6B_000622"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFG9750088.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(43740..44819)
                     /locus_tag="ACGI6B_000623"
     CDS             complement(43740..44819)
                     /locus_tag="ACGI6B_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFG9750089.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSDVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(44952..46298)
                     /gene="pepV"
                     /locus_tag="ACGI6B_000624"
     CDS             complement(44952..46298)
                     /gene="pepV"
                     /locus_tag="ACGI6B_000624"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFG9750090.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(46367..47647)
                     /locus_tag="ACGI6B_000625"
     CDS             complement(46367..47647)
                     /locus_tag="ACGI6B_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9750091.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(47774..48034)
                     /gene="hfq"
                     /locus_tag="ACGI6B_000626"
     CDS             complement(47774..48034)
                     /gene="hfq"
                     /locus_tag="ACGI6B_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFG9750092.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(48148..49083)
                     /gene="miaA"
                     /locus_tag="ACGI6B_000627"
     CDS             complement(48148..49083)
                     /gene="miaA"
                     /locus_tag="ACGI6B_000627"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFG9750093.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(49121..51088)
                     /gene="mutL"
                     /locus_tag="ACGI6B_000628"
     CDS             complement(49121..51088)
                     /gene="mutL"
                     /locus_tag="ACGI6B_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFG9750094.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(51099..53942)
                     /gene="mutS"
                     /locus_tag="ACGI6B_000629"
     CDS             complement(51099..53942)
                     /gene="mutS"
                     /locus_tag="ACGI6B_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFG9750095.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(54102..54836)
                     /locus_tag="ACGI6B_000630"
     CDS             complement(54102..54836)
                     /locus_tag="ACGI6B_000630"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750096.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(54805..55818)
                     /locus_tag="ACGI6B_000631"
     CDS             complement(54805..55818)
                     /locus_tag="ACGI6B_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9750097.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(55840..56610)
                     /locus_tag="ACGI6B_000632"
     CDS             complement(55840..56610)
                     /locus_tag="ACGI6B_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750098.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(56585..56875)
                     /locus_tag="ACGI6B_000633"
     CDS             complement(56585..56875)
                     /locus_tag="ACGI6B_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFG9750099.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(57006..57105)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      57725..57814
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            57867..58043
                     /locus_tag="ACGI6B_000634"
     CDS             57867..58043
                     /locus_tag="ACGI6B_000634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750100.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(58158..58514)
                     /locus_tag="ACGI6B_000635"
     CDS             complement(58158..58514)
                     /locus_tag="ACGI6B_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9750101.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            58891..59271
                     /locus_tag="ACGI6B_000636"
     CDS             58891..59271
                     /locus_tag="ACGI6B_000636"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFG9750102.1"
                     /translation="MTNADGLSESQMQKIARELNNSETAFIFNNLAKNHQENYDVHIR
                     FFTPTNEVPICGHATIAAHYVRAIENQFNNKTVFQKTGAGILPVDIIKKDNDYEIIMT
                     QGKIEVSPPLQQTYQKRLLNAFGI"
     gene            59331..59729
                     /locus_tag="ACGI6B_000637"
     CDS             59331..59729
                     /locus_tag="ACGI6B_000637"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFG9750103.1"
                     /translation="MIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQ
                     VHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKM
                     LVKVIILDNKPVEVKIIGKAVIAFSTNIEI"
     gene            59960..60571
                     /locus_tag="ACGI6B_000638"
     CDS             59960..60571
                     /locus_tag="ACGI6B_000638"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFG9750104.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            60869..61207
                     /locus_tag="ACGI6B_000639"
     CDS             60869..61207
                     /locus_tag="ACGI6B_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HFG9750105.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(61614..63050)
                     /gene="miaB"
                     /locus_tag="ACGI6B_000640"
     CDS             complement(61614..63050)
                     /gene="miaB"
                     /locus_tag="ACGI6B_000640"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFG9750106.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(63298..64734)
                     /locus_tag="ACGI6B_000641"
     CDS             complement(63298..64734)
                     /locus_tag="ACGI6B_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HFG9750107.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT        22343 a        10145 c         7415 g        25350 t
ORIGIN      
        1 attattgtac tataatctat tatttaaaat attatgtaaa aaaatatcat aaaaacgcat
       61 atcatgttat aattactaga gtattatgaa tatggaggcg tagtttgtga tagaaataag
      121 tataaaaaat aaaaaaaatt ctaaaattaa aactttgata cttttattga tagtatctat
      181 atcgatttat ttaactggtt gtactacatc agataatgga aataatcaat caaataataa
      241 tgattctttg tctagtagta tatcaagtga taagaatgta aaaattcatt ttattgacac
      301 tggaaatagt gatgcaatat tgattcaaga tggtaaaact tttacattga tagatggggg
      361 agataatgat gatgagggat taatggtaga ttacctaaac aatcaaggtg taaaagatat
      421 taaatatctt atagctaccc attctcatgc agaccattta ggtggacttg attctgttgt
      481 caaaaacttt aatatagaaa atgtttttgt atcgaatggt tcagcagaaa caaagagtta
      541 tagggacttt ataaatgctt tagcaaataa gggtttatcc ccaagtgtac cgcttgaaaa
      601 taataagttc tatttagaag attcttattt tgaagttcta aatacaaatg ggggagatac
      661 tactaatgaa caatctctag tccttgtata tactaatggg aatgataaag ttttatttac
      721 aggtgatgct gaagaaggta ccgaaaaaga aatactacct aagcttgaga aagtagacct
      781 tttaaaagta gctcatcatg gttctcgtag tagttcatct caagagtttt tagataaagt
      841 aaatcctgaa tatgcggttc ttttagtagg caagggtaat tcatatggtc atccacatca
      901 ggagacaatg aataaattaa aaaaaatggg agtaaaagtg catagaagtg atgagtgttc
      961 agatataata tttgaatcaa caggtgatgg agtatttaca agttgtaaag atggaagtta
     1021 taataaaggc gttagagaag atggaaactc tggaaaatca aacagtaata caagtaaaaa
     1081 tgattctttt aaaaataaac aaaatacatc caattcagaa gaaatagtgt attttactcc
     1141 aaaaggtaag agctatcact ctacaaaaaa ttgtagtggg ctttcaagaa gtaaaaaaat
     1201 attatcaggc acaattgcag aaagtaagaa aaatgaccca tgtgatatat gttatggaaa
     1261 ataacaaaaa aacaaccaat ttatctataa agcttctgaa ctttaaatca tttaaaaaat
     1321 aaaataaatg cataaaaaaa taagctataa tcaatagata acacatttta tctatacgaa
     1381 tagcttattt ttttaagtat tttttaatag ctatttaata actctaaata caccagttac
     1441 aatacctaca atttcaactt ctgagtcatt gagcataatt ggttccataa aatcattttc
     1501 tggctgaagt cttattaaat ttccttcttt aaaaaacctc ttaaccgtag cagattcacc
     1561 atttataagt gcaactacta tctgagaatt taaggctgta ttttttttgt ctactacaac
     1621 gtaatcttca tctaaaattc ctgcattaat catactttcg ccttttactc ttaatacaaa
     1681 attgttactg ccctttacta aactggcagg gaatggaatg tattcctcta tgttttgttg
     1741 agctaaaatg ggttctccag ctgttatttg acctactaaa ggaagctcta ttatttcttg
     1801 attagctcca gaaacatcat ttactttact acgttctaaa acttcaatag ctcttggttt
     1861 agtagggtcc tttcttatgt atcccagttt ttcaagttta tttagatgag agtgcacagt
     1921 tgaagtagat cttaacccta cagcagtaca tatttctctt acagcaggtg gataaccttt
     1981 taatatgatt tgagacttta taaattccaa tatcaagact tgcttttcag ttagatctaa
     2041 atacataaca tcactcattt ccaaacatat tttgttttta tttccataca ctttatattt
     2101 ttaattataa cataatccat ttatatctgc aaacatttgt tcctgatttt taatcgtcct
     2161 caatccattc aaaattcttg actatatcac gttttacatt tttcttatga gctgcataaa
     2221 gctgagtagt tgtgacatta tcatggtcaa gtaagttttg aacatcatat atagaaaatc
     2281 ctgtttggat tagagaagtt gcagccgttg ctcttaattt atggggacta tatccattgt
     2341 ctctagtagt atcaagtgga atagatgtat atttttttac cagtgttctt atagcctttg
     2401 ctgtaattct cttattttga agagacaaaa ataaagcatc tttttcttca tcatttaata
     2461 gttcatctct tgttctttcg ttttgcaaat aatcttttat tacatgttca caagtatgat
     2521 ttataggcat taaaacttct ttcccacgtt ttctatatat tttaaattca cctcttgaaa
     2581 aattaaaaga ggagatattg agttctctaa gctcatttaa tctaagcccg tatgtaacaa
     2641 ataatgctag tatagctttg tcacgtaatt tagtttttct ccaatatacc ttttctttct
     2701 ctgtaagccc ttcacctgtt tctactgatt caagcatctt agcaacttca tctatctcaa
     2761 ggcgcttaat agcatctggc tgaggtttag gaagttttat tggattgaat ccatcagtta
     2821 tattattatc tatctgagag tttctgtata agaatttaaa tagagtagag atggatgatt
     2881 tttttcttgc taaagctcta ttgttatttt caaaaatcaa tgtgtttttt tctgtatttt
     2941 tgtaatatct actgcaataa tcacctaaga ataaatttat atctcttgat tttattgtat
     3001 taaaatcatc ttctgtaatg tcttttacac aatctgcatt tgttaattct ttggtttcaa
     3061 tcaaatatga acaaaaaaaa ttaatatctt ctaaataagc aagtcgtgta gaaactgcaa
     3121 cagaaccttt taaatatata aaataatcct tcataaatac aggaagttta ttttccaaaa
     3181 gtagacattt ttctatgagt tcattttctt tgataacaat attatcaggt ttatctgatt
     3241 tattatgtac ttttataatt ttcttatcat tattcaaaat taaacctcct aaaactaaaa
     3301 tataaatctt ttatattatt ttttgatatt tatactaagt gagtataaat atttaattta
     3361 atcatttttt acatttttaa gagattagca gtgctttaga tgaaattatc actaactatt
     3421 ccctaaatcc acatttaaag actagttgaa tttaagtact cttttttatt tctcaatata
     3481 tttatataaa tatattgaga aattttttat gctctactag aatctttttt atataatatt
     3541 tttatatgtt tctaacgaaa ccaaatatta gtaaaatatg tatttattta actaaagttt
     3601 ttaaatgggc tgactaagct taaaatatag gatattatga gagttactat tgaaaaaata
     3661 aaccctattg ccataaagaa acttataaat atgttatact taactattaa actggatata
     3721 ttcaagttat caaatccaaa taagtatgta attgaactta ttacaaaaat aactataact
     3781 ataaagctca ggccatttaa gctatttttt aaatcagcac tactaagtga catatgtgta
     3841 gatatagaat acattaaaaa cataaatata aaatacttca atgaatatac actaggtgtt
     3901 aacaacatta ttcttataaa attggaaata tcacctaaaa taatgtgtat aaaaccattt
     3961 atatttaaat tattaatcat gcttacatat aactttaaat ctatattttg agtcactttc
     4021 atataactat ctggcagtaa tatcttaaaa attaaaataa tcattattgt tccacctata
     4081 ataggtgcaa ttcctataaa gaaatttcct atactttgat aaagattatc ttttttatag
     4141 ctatgactta catatcctag tataccatca tttttacttt ctattggtct gaaaaactta
     4201 actgaattta ttttatgcat gaaaattaaa cacattatca aatgactcaa ttcatgaaca
     4261 aatgttccta tagctccagt tacaataatt ccagtttttc caaaagatga aaatataaat
     4321 ttattattta gattttctgt aatgctaaaa atcaatccaa atacaataaa tattcctatg
     4381 aaacaaaaaa tatttattac acttattttt aaaatttcga ttatataaat cataagttaa
     4441 atcctcatgt aaaagtattt aactactcta cttcatatcc attacagctt aaaattttac
     4501 aagcattttc tatatccttt tcttttacta agatatagtc tgtatcataa gtagaaattg
     4561 caaatatgct tattttgttg tctgctaaca aactacttaa cttagataaa attcctacaa
     4621 gtgaaaagtc taaaggacct agtatcttaa gtattctcca ctccttttct gacttaacat
     4681 cagaaggaat attattattt aaacatacaa cacttaattc atcatcagtt ctagttatag
     4741 agtaaaactt ctctgtatct atccatgttg gtattttact gtcattattt aatctacata
     4801 ctgcatactc ttctaccaat aattttaatt tcataatgta tatttcctcc aatcttattt
     4861 gtttacttaa gattatacat taaaagttac ataaaataaa acatataaag taaaaaaggt
     4921 tcactatagt gtcctatttc tatagccaac ctttaaaact taattaaatt ctaataaaga
     4981 gatatattat ctaggttcaa ttataagttt aatggctgtt ttcttatcac cttcgatttc
     5041 tatatcagta aatgcgggaa tgcaaactaa atccattcca ctaggtgcaa caaaacctct
     5101 tgctattgca attgatttta tcgcttgatt tagagctcca gctcctatag cttgtatttc
     5161 tgcaacgcct cgttctctta gtactccagc caaggctcca gctacagaat taggattaga
     5221 ttttgatgaa acttttagta cctccatttc aacatctcct tgttaatttg ttaaagtatc
     5281 cattacctaa agtactaaat cataattgcc atttactaat tacaatcatc atttaaagta
     5341 tacttttata aaaatatact tctataatcg ttttaagaaa acctttattt tttttaacca
     5401 ttttattatg aaaaaactac aaatttcttt atattctata agcattttcc aatatttcta
     5461 aattttttat atctttgagc tataatttcc tctttatcca tatctttgta atgatttaat
     5521 ctttctaaaa tgtaagcttt tatttctttt gaaacagctt ctacatcttt ttgtgctcct
     5581 ccaagaggtt cttttataat cttatctatt atttttaaat catataaatc attacttgta
     5641 agcttcataa catcacaagc ttctttagct ttagatgaat ctttaaatag aatactagaa
     5701 agcccttctg gagaaatcac agaataaact gaatgttcta acatacatac atcatttcct
     5761 actccgatac ctaaagctcc tccactacca ccttcaccaa ttacaaaagt gattactgga
     5821 acagaaagtt tactcatttc aagtaagttt cttgcaatag cttctccttg tcctctttct
     5881 tctgcttcta gaccacaaaa agctcctgat gtatcaataa acgttattat aggtctatta
     5941 aatttttcag cttgtttcat aagtcttaat gcttttctgt atccttctgg atgaggcatt
     6001 ccaaaatttc tttttatatt ttcttttgta tctcttcctt tttggtgtcc aactatagta
     6061 acattaaact ttcctatctg accaattcct ccaaccatag aagggtcatc tttaaaaagc
     6121 ctatctccat gaagttctaa aaaattagaa caaatatgtt ctatgtattc caatgtcgta
     6181 ggtctcttta tatctctact taatgttacc ttttcatatg ctgatagatg tgaaaaagca
     6241 tcttccttaa gcttttccaa tttctcatta agtgaattta ttttatcact taaatctata
     6301 ttatttgcct tagaaatagt tattaattgt ttaatatcat tttctaagac ttttattttt
     6361 tcactattat tttcaatcaa aacagttcct cctttatgcc ctatttcttg tgcatagcaa
     6421 gaatttgata taatacttcc ttcatttttc ttctatctac tatcatgtca ataaaacctt
     6481 tttcaagtaa aaactcagaa gtttgaaatc cttcaggaag tttttgattt attgtttgct
     6541 ctattactct tggacctgca aaacctatca acgtatttgg ctcagaaatt ataatatcac
     6601 caagcatagc aaaactagca gttactcctc cagtagtagg gtcagttaga actgatatat
     6661 atggcaatga tttttcttca agcttagaaa gtgcttgaga agtctttgcc atctgcataa
     6721 gagataccat accttcttgc attctagctc ctcctgaagc agaacaaata attataggta
     6781 aattattttc agcagcatac tctattgaat aagttatttt ttccccaaca atagaaccca
     6841 tacttcccat cataaaatca ggattcatta cacaaataac tgttttaatt ccatttattc
     6901 ttccataacc tgtgacaaca gcttctgact cttttgtttt ttccttataa gagtctactt
     6961 tttctttgta tttaggaaaa tttagtggat tttgaaattc tacatcagaa ttaaactcca
     7021 taaaagttcc tttatcaatc aataaattta ttctttttct tgctcttaag ccataatgtc
     7081 ttgaacattt tggacataca aatgaatttt cttctatgtc ctttctaaat acatgactat
     7141 cacaaccttt gcaataggtc caaaatttat catctacact attcttttta aagttgtcat
     7201 ctaaagaaat cgtaacatac ttagattctt ttgatgataa aaattttttt atcataatct
     7261 atttactcct tataatgtag caaattcttt cttcataaag acatcttcta taaatttggt
     7321 atgatgagta tcttctctaa actcctcact tgttacaagt gccttttgaa attctacatt
     7381 tgtattaata ccttctacaa taatttcgtc tagagctcta tatattctac ttatacattc
     7441 ttctctagtt tcagcccaac atattaactt acctatcata gaatcatata aaggtggtat
     7501 agtatatcct gcatatacaa aagtatcaaa tctaacacca aaacctccag gtaaatttaa
     7561 tgattctatc ttacctggtg atggagcaaa attctttgat gaatcttctg catttattct
     7621 acattcaata gcatgacctc taaaagtaat atcttcttgt ttaaaaggaa ttttatcccc
     7681 attagctata gatatttgaa gttttataag gtcaagattt gttaccatct ctgtaactgg
     7741 atgttcaact tgtattcttg tattcatctc tataaaataa aagtttttat ctttgtcaac
     7801 tataaactca atagtacctg caccgatata acctactcct tttactatat ctactgcaat
     7861 tttatgaagt ttttgtcttt cttcatctga aaggtatgga cttaaagatt cttctattac
     7921 tttttgattt cgcctttgca tagaacaatc tctatcacct aaatgtattg catttgaatg
     7981 tttatctcca aatacttgta cttctatatg tctaggattt tctataaact tttccatata
     8041 taaatcacta ttagaaaatg ctgctagagc ttctgaactt gccatatcaa aatttgaaaa
     8101 aagttcttct tcttttctaa cgattctcat accttttcca ccgcctccac ttgaggcttt
     8161 tatcataaca ggatatccaa tttctcttgc aacttctaaa gcttcttcct tagttttcaa
     8221 aacagattct gaaccaggaa caacagggat attcaattcc atcatagttt ctcttgctct
     8281 agatttatct cccatcaaaa tcatttgttc tcttgttgga cctataaaaa taatattatt
     8341 atcttcacat tgctttgcaa attcaggatt ctctgataaa aatccaaagc ctggatgaat
     8401 agcatcacac tttaatttaa gagcaagata gattatattt tctatattat tataactctt
     8461 acttatatta tttggtccta tacaaataga ttcatcagct atatatctat gaaaacaatc
     8521 tttatctatt tcagaataga tagcaacagt ttttatacct agttctttac atgttctgat
     8581 gattctaact gctatatcgc ctctatttgc aactagtatt ttttttatca acttaaatac
     8641 ctcctactac aatggtttta tcttaaataa aggttgatta tattctacta gttcttcatt
     8701 actaactaat acttcaatta tttcaccttt aatttcactt gttatctcat tcataagctt
     8761 cattgcttct aatatacata aagtgtcgcc ttcttcaact acatcgccaa ctttaacaaa
     8821 gctatctgca tctggacttg gagaatcata gaatgttccc atcaaaggag ctactactac
     8881 tgatagattc tcattagatg tgacaggtgt atcgtttaca actggctttt ctaaagaaag
     8941 atccactact gattcttgtt gtacacttaa aacaggtgaa cttgtagatt gagctttttt
     9001 agatacttct aatcttaaat cgctactttc aagttttaca tattctaagt tagttgagtc
     9061 tatggccttt aataattcct taatttcatt gatgttcatt ttgttacccc cttgtattat
     9121 tacctggtgc taatattata tcacaaaaaa caaatttata aaagaaaaag tgctgactag
     9181 agaatcaaca catcaatata tcttttaatt ttaaattaac aaccagtaac ttatatttgt
     9241 tttaattttc cattattaat ataaattata agtataaaat gaataaaatt caatattttt
     9301 tgcaaaatac aagagaatac aaattaatac ctcgggaatt tttaagacaa ataccaatat
     9361 tatgcaattt agacttcgct ttctttcaaa ttagtatcat tttctttatt tttacatagg
     9421 atgtacttta atttagctat caaaacattg ctatataggt taaaagttat tagaattgca
     9481 ggaattataa agtaaactct agcacctata aaaccaaaaa ctactgtaat aagtattaat
     9541 cttttattta aagtaaaaaa aggtatatta aaaatacttt tgtcggatgt ttctggagta
     9601 taagaatata taactccaaa aatcaaagta agcatacaaa gtggaagtag tgtataactt
     9661 gaaattattc ccaaaaatcc tatactttca aaatatttgc tagcactatt tgtataaaca
     9721 aacatgtatc catttaaata tgcgcatata catgatataa tgccaaaagt tccccctaag
     9781 gattgagtgt accaattaaa aaatttagat tccatatacc tcacctccta aaactgtata
     9841 tataatgata tgcatttaaa tagtaaaata tatctttata tgtttctgaa taaaattttg
     9901 ttaatttcat taatttatgt ttaagaaatt tgaagtatag tgtagtatat attaatgtag
     9961 atgaagtttt atagtactta tttcatagtg cttaatcaag ttatcttatt atggattcaa
    10021 aagttgtttg tagggtttta tattttgttg agctgaaaaa tttattataa atgaagggat
    10081 gttttcgctt tggtagatac attagaattt ggtcttaaaa ttttattttt tatattatca
    10141 ataatttgga tgggtaaaat aatgatttta agaactgata agcaaatagt aatcaatcct
    10201 ttgcttattg gtatatctgc aatccttgtg atgttgcata cctcacagag taacattgaa
    10261 ttttttggat tagatgtcca atatataaga atagttttgt atataatata ttcactgata
    10321 attttaatag gaatatgggc tactaacaga agaaatggaa ttttttagat aatctatttc
    10381 tataaaattt aaaggttgct agtaacaaat cactaatatt agcgttctgt tactaacaac
    10441 ctttattttg attatacact taaattgaaa atagaatgat tgaataagtt gacagattat
    10501 tttacattga cagtaattag aacttcttga gatgtattta aatcattttt gtcgcttagt
    10561 ataactttta ccactgcttt acctgtttta ttacctttaa ttatcatatc ttcacatgaa
    10621 agtacactct tatcacttac agatacaata aaatttaaat cttttggaag gttatcagta
    10681 acttcccagt atttataata actatcaatt ttcttcgtaa ttatttttct caaatcaata
    10741 ctttcacctt ttgctatatc aacattatga tatttagagt ccagtttata ctgattatct
    10801 tttgtatcaa taaaatttaa atcgcaattt acatccacac cattatgaga aagagaattt
    10861 actacatcgt catcaatcaa ataattattg gcaagattta ttgaatccat atcttttaga
    10921 cttaaagctt ccttaggaac ctcttttata ttattattcc aaaaaataaa attgtctaca
    10981 ttttcaagat atgatatacc ttttaaattt gaaatactct caccagttgc ataaaattct
    11041 ccatctaaag cctttatatt gcttatagtc atctcatctg gatttatata tctttttaca
    11101 actgctctta aatttgcatc aggaaaaata tctttaagtt gattttcgct caaaacctta
    11161 tcatttgaat ttattggact taaagctgct gcagaaataa taaatggtat taaattagct
    11221 aaattcatag taccctccca cataaatttt ttattataat aatttaattt ctatgagagg
    11281 gttctgtttc cttgttatat atacctactt tcgtacactt tattaatata aattaaaata
    11341 aatttctact attaaactta taaacatatt actagttacg ttttttagct tcacatctaa
    11401 atattcgcat accttgtgac ataaatttgt cttcatactc tgttgtaaca ttattttcat
    11461 actcactatt tcctaaatca agagaaatat tctttaatag ccaattattt gcagcaattt
    11521 catttaaact aaactcgaat aatttttcat tatctgtttt aaagtgaatt tcgccatcat
    11581 cttccaaaac cctattgtac atttctaaaa atccagaatg agtgagtctt cttttactcc
    11641 atctattttt aggccatggg tcacagaaat ttatataaat tctcgataat tctttagctt
    11701 caaagtaatc caaaatattg cttacatctc cccataaaaa tagtatatta ttcaaattag
    11761 aagcatctgc tttttcaaca gcttttaaaa gaacttcttc cttaagttcc atggcaatat
    11821 aatttatgtc agggttctgc ttagctaaag ttgttataaa cttacctttt ccagtcccaa
    11881 attcaacatg tattggatta tcatttctaa actttaaatt ccacttgcct tttaatttgt
    11941 ctatatcgtc tcttaaaaca tattttgtat aacttaaaag cttttcatct gcaccttttt
    12001 ttcgtcttct tctcacaata attctcctta catataaaaa attagtaaaa tacattttta
    12061 ttatacatta tctcaccaaa aaataaaaag aactctctta aaatagaaat ttatttctat
    12121 aaagagagtt ctaaacttac taaaaactta atcttagtgg caccctgaac aaccagagca
    12181 tcctcctcca ccttgatctt tcatattttc aggaataact ctaaatccaa atcctgtatc
    12241 ttctattatt atatctccat actgactata ttcatcagag ctcattacaa attgtaattc
    12301 tccaacttca taagttaagt catcttcttt tttctcatct aaagctaatc caaaagaagg
    12361 acctccacaa ccaacacctg caaagtatac tcttatatta ttaggtttat cttgattgtc
    12421 ttttaagata ctttttaaag tatcaatagc tgtttctggt aaagtaattt tcatcaaatc
    12481 attccctttc aaatatttta ttaaaacaat atctattata tacccataca aggtacataa
    12541 tcaattagtt tttgcatatt ttactaaaac ttctacctaa tattacctaa ggctttaaaa
    12601 tattttatat aatattttat taccttaaat aaatacccta tcataaactt agtaaacaaa
    12661 taaaattatt attctaaaaa atataataat tttatttgtt gttttaatat tataacacat
    12721 ttgataatgt ccaattcata tatgcaaaat ttatatactt taaaatatct aagaaattat
    12781 tcaataaaaa aatactattt tttaaaaatt atcattattg ataaagctct actatttata
    12841 ttttttagtg tattcatgta taataagtaa aagcacaacg gtatatactg ttgtgctttt
    12901 ttgtaattaa taaacaatat tttggtctat aacaatgaaa aatggctact ttccataatt
    12961 gatttcatct gacataagca aaaagggtgt ccagctgggt caatcataac tctccagtca
    13021 tcagtaaatt gctcctctgc gattgtagct ccacaatgaa ttgcatattg aactgcttct
    13081 tctaaatcct taacagcgaa atccatatga gccatttgct gttgagattc aggtttttct
    13141 ggttctatta tgccccaagc ctttgttttt tgcaatataa agaaaaaatt atatatttta
    13201 atattttata cttgttgaaa ttgtgtaata aattcaactg gatacatagt gcgttttgaa
    13261 atttcttcta aagctaaacc ttgttttaaa agcctcttta tgacgctttc catactttct
    13321 gaaatttcta taatattttt atcaaaatca taaaacctaa aagttctttg accccatggg
    13381 tattctttta tataatgcaa aaattcaata tttcctattc tatttatctt actttcccat
    13441 tgtttgatgt cattaacctc aaaatacaac tgaaagttat ttgattgttg atatattggt
    13501 aaatcatatc caattatagc tttataatta ctttgtaatc caaatccatt ttcaaatgat
    13561 acatgttctc ctaaatctaa tgtgattttt tgctccataa catcttcaaa aaactttttt
    13621 gatttattta tgtctttgac aattagcaat actcctccaa atttcatctt atcactatcc
    13681 tttctttgtt ataagtatta taacttaaaa ttttgcaatt tcactgtaaa aatcagacat
    13741 attagctcgg tatttttgag gagttattcc acagacaatt ttaaaatctt taataaaatg
    13801 tggtatatca tagaatccta attcattaca tatagacatc catgaagttt tattattgtt
    13861 tagaagtttt atagatttat taatacgtac caaacgagaa aatgacttca tactcaaccc
    13921 tagatactga ttaaagagtc tgtttaaatg cctagaacta taaaatactt ttttagaaat
    13981 ttcttgtgaa gatatatttc cagagttttg aataatcaac tgaattgcca aaataaactc
    14041 atatgagtat tcaattttaa tattagaaat caataattct tccaacttta aaatcaattt
    14101 atctatagat aaagattcat taaatatttt tttaatttct aaatctaatg aagtattaat
    14161 tatagaaaat ggaacaactt tatcaataag ttcactttgc tgtataccta taagcgggaa
    14221 aaaaccagca ggttgaaatt ctataattag tataacatca catctattag caatatttcc
    14281 tactgttttt ggtttagtag ttggaccaaa taatagacta tgtaaccctg tggcatcata
    14341 aaaaaacacc aaagttacac ttccatgagg aataatcgtg taattatcag aaattattgt
    14401 ttgatttgga aacgatatag taaaattcga tatccaattt cttaattgta tacaaggcaa
    14461 gagataaaca aaattgtctt cacaaactat aagtgaccta tttttattta taaaatgaat
    14521 actttctttt tttaacatat attatatcct gtcctatcgt gaatatttat taaagtaaga
    14581 agaatccata ttatctctat atatcattaa ctgcttttaa taatagagat agtatgaata
    14641 ctcattagat atttaataaa caaataaaat taaaataatc aataaaaaat atagtacttt
    14701 tatctgttat tttaatatta tagcacattt atagtattta acttatatcc actttacata
    14761 tgtctaattt atataattaa aaatgcttat ctcaaataca ataattagtc cttatacaat
    14821 taaaccatct gaaactgtaa tagtacgact actacttttg gctgcttctg gtgagtgagt
    14881 taccataatt atagtttgtc cattgtctct gtttatttta ttcagtaaat ctattatttc
    14941 tatccctgtt ttactatcta aatttccagt aggctcatca gcaaatagaa tctctggttt
    15001 tccaattaaa gctcgagcta tagcaacacg ttgttgctga ccaccagata actctcgtgg
    15061 tgtatgttta cgtctatcag ttaaaccaac tatatctaag atttcatcta attgattttt
    15121 ataatctttt aaatttttac catctaataa caaaggaagc attatatttt cctccacatt
    15181 taaatttggt attaagttat aaaattgaaa tacgaatcct atatttctac gacgtattat
    15241 gctcattttc tcatcattaa aatgtgatat atcagctcca ttcatataca cttttccgct
    15301 cgttggagta tctaaaccac ctaaaatata taaaagagta cttttaccag aaccagattg
    15361 acccatgata gaaacaaatt cacctttcat tacttgtaaa gatacctctt ttaacactcg
    15421 agttgttgta tttccaattt tatattcttt gataatattt tttgcttcta cagctaaact
    15481 attatgtgta attttcatat taacctccat aattattcaa acttaatttc ttctatcagt
    15541 ttcattttct tcccttttat aataggcact atcgaaccta ttaaagttac tataattccc
    15601 aataacccaa caataataaa tgttttaaaa tctaaatctg gtttcattga aatttttggt
    15661 cctgcaacta taaagatagt ttgaatctca agtatagata tcacaatacc aataacagct
    15721 ccaagtaaac ctgaagtaac cccttcaatc aatgttactt ttatattttg tttattactt
    15781 agtcctatag atttatacat agctatagaa cgtctttttt gaatataatt aatcagtaga
    15841 ttattaataa tacctactgt agcaagaaga aaaatgaaat aggtcatctt attcatagga
    15901 gacaaaaaac tacttatagt atttaaagaa tcattattaa attcttcaac tgtacgactc
    15961 caattatatg tatctccaaa taaatatctt atctgaatca taacagcatc tggattgact
    16021 gctgtatata ctaaaaaacc ataattagtt ttatcaaaat cattaactgc ataatgtgat
    16081 ggtataacag cttctacatc atttgcacgg gatttaaaac tacctactat tttataagaa
    16141 aaatcatact tatcattaga tagagttatt gtatctccta tagataagcc tacttttttt
    16201 agagtatctt cattcaaaat aacagaacgt ttatcattaa aagcttctat tacttgtttt
    16261 tttatctcaa aatttgtata attgattcca aacatagagt tatatagttt tatattatct
    16321 gtaccctcta gtcttgataa tgttacacca ttaccagaaa tctcattatt cataacataa
    16381 agaggtaata tttttttaat tccttccata tgtctaacat cttcaataaa ctcttcattc
    16441 atatttccat ctgcaaaacc ttgtaattct gcgtcacgaa acacatctgt tatataagtt
    16501 ttgacaaagt taccaacaac acttattgca ataactgcag aaatacttat aaatagtaaa
    16561 gtgatattct gtattatatt tttgttattt ttcatatttc tagcagctaa tttaccctca
    16621 ttccctaaaa tatttttata aacaaactca aaaactatag acatgatatc agtaataaga
    16681 ggaatcaaca caatagtagc tacaattaat cctagtaatg aaaatccacc agctagatac
    16741 aatgtatttt caggggatat tcttggaagt aaaatagata aaataaacat tatacttcct
    16801 ataaaaagta tagaacgatt tgaaacattc ttttcttcta ctgttcctag tacaatattt
    16861 tttatcggca aatggctagc ttttttaaca ggtatatatg cactaaatga agaaacgatt
    16921 attgctacta taacagatat aatcactcca atgggtgata ttactactgg aattgatatt
    16981 ccttgttcga gagaatttcc taacccatga agcattaaat ttaaaacaac tacaccaata
    17041 ggaatagcaa ttaatcctcc tatacttccg tataatatac tttcaagcat caatatacga
    17101 gttacagttt tttcattggc accaatactt ctaaatgttc caataaaagg aagacgttct
    17161 aatgtaataa ccttataact gctgtatatt ataaatatac taagggttaa tgcaaaaaaa
    17221 cttattaaaa agaaaggcat tgtcttttgt ctagcatctg aaattatctt agtttcattt
    17281 atagtatttg ataccatata tttttcagta gaaagttctt cggataattt atttactaga
    17341 ttttctgtaa tttcttcttc tgactctata agaactcttg tatatccact gcctttattt
    17401 ataatttttg atagagtctc tttgggtagc agtgcattta cacctcttgt gttcctcaaa
    17461 aatactgtat cataagatgc aatatcacta acttgaaaag tataagactt tccataaatc
    17521 tgcaaagtaa tagaatcacc tttttttatt ttatattttg atgtaaagcg atttggaaga
    17581 ataattttat caccagaaaa atcagtaata ctatcgccat tttctaatcg tggcttgttg
    17641 attttattta gttgacttaa gtctgctgat ataagactaa attcttcata gtatccccct
    17701 ttattataaa tagcagagct ttccaataca ccaacctttg attttatagt atttaaatca
    17761 ggtatgtctt ctaaattggt taatatatct ggatttttac tttggacaga tatagtcgct
    17821 gtaccataca taccttttgc catttttctt tgtgcacttt cataagataa tccaattgag
    17881 aatgaaacaa aaagtaaaac tgttgataaa actatggaca ataacataac tgctgttcta
    17941 atttttcgtt ctttaatatt tgtgaaaata tactttaata taattttcaa acttacacct
    18001 tcttccctta tctaagatat ttcaatctaa tatttttata ctttaatcta aattaaatct
    18061 ctaatagact cctccatact tttatcattc tatttataat atttttaatc aaatcacccc
    18121 ttaaataaaa aagattgata aactactata taaatataat aatctatcaa ccttaatata
    18181 ttttttattt aaccttaatt aaccttaatt aaataggaaa ggtgatataa aatgagctac
    18241 ctaatccagg tatactttca acagatatat tccctccatg aacttcaata atttgcttcg
    18301 ttattgctag tcctaaacca gttccttcta ttttttgttt tgtattgctt cctctataat
    18361 aacgattaaa caaatttgat gcttcttctc ttgacatccc ttttccatta tcagatataa
    18421 tgatttcaat tctttcattt aatgttacat gtacacaaac tttagtattt ttatctccat
    18481 atgtaaaagc attgacaata agattactaa aagctcgttt taatagtgta caatcaaaat
    18541 caaagttaat attatctata ttactcttaa attcaactat atttgattca tattctggat
    18601 aatttaaaat atcaatggat aattctttca agaatcttac taaatcataa ttatttttat
    18661 ttaaagggat tattccattt tttaattgat aggttaattt taaatcgttt ataagtgtat
    18721 ttgtatattc tatgttcttt aacatgattg aagcatattt atatactttt tcaggtgaat
    18781 aattagtact actatctgta agaagctctg catacccttt tattggtgaa agtggagttt
    18841 ttaaatcatg agttatattt gaaatccact cttctctcat aatatcagtt ttattttgta
    18901 tctcatcact tttatgaatt tcttcattta aaatatttaa actattatat atatcagcaa
    18961 aaacaccttt aatattaact ggaagatagc ttcgttttga aatatcacta acagcagatg
    19021 taatatttga tatgcacttt gttacccagt aaccataagc aattccagaa accaaaatta
    19081 ttaaaaatat aatccctatg attgaaaata caattgactt accacctata aatttacctc
    19141 catcaaaaaa catattcact ttagatatat ttaatggaaa atatataata taaatatagt
    19201 catttttatt atttttaatt gtattaaagt atacagttcc attacttgaa ttattttggt
    19261 tagctaatgc taataattca gaattagaat aataatcttt atgctctttt ggttctaaaa
    19321 acccataaac tctatttcca ctagaatcta aaatttgcaa ccacaattga ttttctcgaa
    19381 gtttttctaa tccagactgt tttacttgtg gcttattatc tataaatacg atttgttcat
    19441 taaagttttc tgtaaatttt ttcggccagt cactacgaat aaattcccca tttggtttct
    19501 gaatagtaat aagtgataag aaaataccta ttactgtcag gattaagcaa ataaaaatca
    19561 tgaaaaaaat aatgtatgaa tgaaatactg ttttataact aggtttatgc atttaatcaa
    19621 ctcttttctt aaacttttat tttagtttga ttgttgctta tataaaatag acaataatta
    19681 ttgatatgta aaaatttatt taaaaataca actatctaca taaattatat ttatataatt
    19741 acatgttatc aagctgtctg ttgtatttaa taaatcattt tgataacata taaaagtaag
    19801 ataaattttt ccattaaata ccttataatt atacacaaaa tctctatata cgttaaacaa
    19861 tatagagaaa attttcatag aaccatcttt ataggggata ctttataaaa gaataaaaaa
    19921 attataatat ataaaaaact tcataggagt gtaacaacat cagtaaagat atgaaaacac
    19981 tatataaaaa ttcaaaaata ttaagaataa tgaaatactt gaaattgctt atatcaagaa
    20041 attttatata atataaactg gtcaaatatg ttataatatt aaattgtgac aagttcatgg
    20101 aggtgtttat atgaaaaaac aaaagtcgcc aattttactt gaaatgcttg gactattttt
    20161 tggttgtata tcaatgagta ttggtataaa tatgttttta aaaccacata caattgctcc
    20221 tggaggactt agtggtctat cattagtact aaacaaggtt acaggacttc cagtatcagc
    20281 tatcatgtta attataggtg tcccattagt aatacttgct tttagaatta tgggtacaaa
    20341 aaattcatta aaaactttat ttggaacagt tgtattttca gctatagttc aattaacaga
    20401 cccattatca aaactaagat ttactaatga cttattattg tcttctattg caggaggaat
    20461 cctagttggt ataggtttag gaataatgtt taaatcagat gcttctactg gaggaacaga
    20521 cttaattgca ctcatactta gtaagaaatt cccaggaata aaagctacaa aattcatgtc
    20581 atgcctagat ggaatggttg ttatatcttc tggtatagta aatcgtagtt tagaaactgc
    20641 tctttactct ggtatagcac tttgtgtatt aataaaaata gcagatatga taatggaagg
    20701 atttgaccat tcaaaagcat tttttattat ctctgatgag cctgaaagtt taagacaagc
    20761 tataactgaa gaacttgata gaggtcttac tattcttgat ggtaaaggtg gatacactag
    20821 agaaaataaa gaagttctat tagtcgtagt ttctaaaaaa caagaacttt atctaaagag
    20881 attagttaaa agaactgacc ctactgcatt tgttattgtt acagatgtac atgaggttct
    20941 tggagaagga tttaaaaatt tagagaacta ataaaaatta atattttaag tatatatact
    21001 attttaatta aaatacgatt tattcaaaaa gagtgtctca taaacttttt agttcatgag
    21061 acactctttt ttgtataaaa tcaaatgcat tttcagtatt ttaacaataa aaacaggatt
    21121 atctatattt tgaggcaacc actttattga aattttattt agaaaacaat cattactaaa
    21181 taaccagcat agatattaaa tgattatatc tattgttttt tcaattcata ttgataacaa
    21241 ggaacatttt ttacagtatt tccagtatga atttttttga tatactttcc tgtacattta
    21301 aatcctaata aatctaatga ttttgtagaa ttttggtttt cttcataggt tattgcaatt
    21361 attttattta agtttagttc cctaaatcca tactgtatta ctctttttgt tatctcagta
    21421 gcaaaatcat gtccccaata gcttggattt atacatatac ctatttctgc ttctaatgct
    21481 ttaggattta tattattaaa ccccatattc ccaataactt tattgtctct tttatatact
    21541 atagcaaagt ggccaatttt ccctttttta tatggattta gaaaaaatga ttttataaac
    21601 tttcttgtat cttctaaaga tttatgctca ctaaatggta aatgttttac tactatttta
    21661 tttttataac agtcaaaaaa atcttcaaca tcatctaatg atgcaggtct tagtatacaa
    21721 cttctagttt ctaaggtttt catttgagga tattcagaat cagacatttc taatccccct
    21781 ttatacatta atctaaatat actgttcata tttaaaatac gatattaatt atataaatgt
    21841 tctcaaatta accttatttt tatttttaat aaaaactaat tactatatga atctttcaaa
    21901 tttacgaatt tatctctatt tttatattaa attacttgtg aaatatgtat tctaaattat
    21961 atattattag atatcatgct taaaaattct ttggttgcta aggataaaga tatatctttt
    22021 aaatggcaat aacctatatt tctcttaggt attggctctt ttatatctaa tttaaacaat
    22081 ttctcatttt ccaaataatc tatgctaaat tcttctataa cacaagacac tccaagattt
    22141 atatacgcaa attctaaaag taattcatga gaacccaatt ctatatctgg gtttagtttt
    22201 ataccttttg ataaaaaata ttcatttaca taaagtcttg aatttgcttt attttctagc
    22261 attattaaag gcaatgtgtt taattcttcc aatgatattt tcctaccttt gtattctatg
    22321 taatcatttg cacatacaaa tatatcatgt atttcaatac actcaacaat atttagtgta
    22381 tcatcttcta ttggcatgtt tataattgct atatctatat tcccatcttt gagtaaagat
    22441 ataagttctc tactcgttct atttattgta gatacatgta tatgtggata cttcttatga
    22501 aaaatttcta aatattttaa caagaaaaaa cgtgcagctg tatctcctac tcctattttc
    22561 agagaaccgt attctaattt tttaaattta tttattctat cttctcctga tgatattaac
    22621 tttaatgcag tagtgacatg ttcacttaga attttcccct ctggagttaa ttttactcct
    22681 ttattgtttc tataaaataa taaagtatct aattgttctt ctaattgctt tattgactgg
    22741 cttacagctg gctgtgacat aaacaattcc ttagcagcaa gagaaaaact cttattattt
    22801 acaactgcat taaaaacctt ataaagttca agttttatat tcatatataa ttactcctta
    22861 tgtcaaatat tagtattatt tattttactt ataattatat ttgaattata atatacatgg
    22921 tactaatatg acaatattaa ttctttagga ggattttaga taatggatag attagttgga
    22981 actgtttcaa gaggggttag agcacctatt ataagacaag gagatgactt agttaaaata
    23041 gttgttgatt ctgttttaaa tgcgtcaaaa agtgaaaact ttgaagttag ggacaaagat
    23101 gtaattgccg ttacagaagc tgttgtagct agagcacagg gaaattatgc tcacgttgac
    23161 aatattgcaa aagatgtaaa agataaattt ggagatgata ctgttggagt tatattccca
    23221 attttaagta gaaatcgttt tgctatatgt ttaaaaggaa ttgcaaaagg atgtagaaaa
    23281 gtcgtattaa tgttaagcta cccttctgat gaagttggaa atcacttaat aagtattgat
    23341 gaacttgatg ataagggaat aaatccttgg tcagatgttt taacagaaga aaaatacaga
    23401 gaactatttg gatataacaa gcatacattt actggtgtag attatgttga ttattataag
    23461 aatttaatca aggattgtgg tgcagaggta gaaatagtat ttgcaaacaa tccaaaaact
    23521 attcttaact atacaacaag tattttaact tgtgatatac atacaagaca aagaacaaag
    23581 agaattttaa gacaaaatgg agctaaaaaa gtatattcac ttgatgatat aatgacagca
    23641 agtgtagatg gtagtggtta caatgaccag tatggacttt taggttcaaa caaagctaca
    23701 gaagaaacag taaaattatt ccctataaat tgtgatgaag ttgtaaacaa aatccaaggt
    23761 aatataaaag aaataacagg gaaagatgta gaagtaatgg tttatggaga tggagctttt
    23821 aaagaccctg taggtaaaat atgggaatta gctgacccag tagtatctcc tgcttataca
    23881 aaagggctag agggaactcc aaacgaagta aaattaaaat acttagcaga taatgatttt
    23941 gctgacttat ctggtgatga gttaaaagaa gcaatttcaa aatacatagt tgaaaaagat
    24001 aataaatctg atgatttaac aggaaacatg gtttctcaag gtactactcc aagaagatta
    24061 acagacctta taggttcttt agcagattta acatctggaa gtggagataa gggtactcct
    24121 attatttaca tacaaggata tttcgataac tatacaaaat agtatgtttt aataagattt
    24181 aatttttttg gattatatat attaacatga aaatttatta aaacctctta atagatatga
    24241 cttttcaaga gttatatcta ttaagaggtt tatttaatgt tcacagataa gatatattta
    24301 aataatttaa acaagattgt cagaaagtta tataatctta aaaatttata taactctaat
    24361 tcttatttat ttctgaaata atatcactct tcaaaatttg tcttactgga ataattacag
    24421 caactatact aatagtgata tttaaaataa tcatacctat gtatagaggt gtatttattt
    24481 taagaattgg atttatatat aaatatacac gttttaccat aaaataaatc ataaattgtc
    24541 caataacact tccaattacc attataatac ttgcatagaa tccatataaa aaaccttctc
    24601 ttatcatcat aagtaaaaac ttattatccg taatacccat agctcttaaa attccaaact
    24661 catgtcttct tgaaaaaatt aagtagctta ctgtattgat tatatggaac aaacttattg
    24721 caagaagtac aaatacaata ccatatataa ataagagttt ttgatttaag taggtattat
    24781 ttttttcaat agcaatggta taatctgtta cgatacatct ttttacatca cttacaacac
    24841 tttttatgtt ctcagacaat tcctcactgt tactatcttc tgtctttttt atacttactg
    24901 aattataatt ttcgatatca aagttatttt tcatttgccc attcgtcata actatatcca
    24961 taccataatc accaatgaaa tatatgttat ttgccatcac acgttttaca gtagcagcaa
    25021 ctacaaattc ttttgttgta taatcattct tattttcttc tttaaattta acaatttcat
    25081 ctgttggttt cattgtcttt ggcactttaa cttttattgt atctcctgga tttatatcaa
    25141 ctgcatcata catcccagtt ccatccatag gtagtctaac tataacctta ttatttttaa
    25201 ccatatcatg ggtattaatc tccccatcta taatataaga atttaatcct tctaacatac
    25261 cattgttata accataaata tttgttttta aaagatagcc accttcattt tgctttgtac
    25321 aaataccatc aaaaaaagtc tttatcctgt aatctttttc aagaggttta aagaaatttt
    25381 tccaaaatag ttgttcttct ttaatttgca tagctccaaa taaatatctt actggataga
    25441 cttttgatat tccctcaaca ttgtttaatc ttgaaatttt atcttttggt atacctacat
    25501 caaaatctgt tgtttgcata gttatttggt agtcagaatt taaaccatca tcagctttca
    25561 ttgtaagttc attattattt ttagtaagct gtatagagta actagaacat aaaaatataa
    25621 ttccaccaag tgataatgaa aacaaaattc caataaacat tccctttttt tgagtcatgt
    25681 atttatgaga taatactttt gtcatgttag agtgttttac actttctaat attctctttt
    25741 tcttattagt aatcttaaag tttttcatag attctatact agagtattta ttcaactgat
    25801 gtacaacacg tacagcaatt aaaattaaca acaataagat aaataaaaag cctaaataaa
    25861 tagatttttc acttataaaa aatgctcctg gaacaatatc actactcaaa aatatatgtg
    25921 gaaattgttc atatattgta gacgccactt caatacctaa gaagcaacct ataggaaatc
    25981 caacaataaa aagtaaaaat aactctaaaa atagtagaaa taaaagttgt ttattattag
    26041 cgcctaatac ttctaaaacc ccatattgtg atattctctg aagtattgaa acatggaaaa
    26101 tactatatac tgctgctgca ccaaacactc caacaagtaa tataattgca tttgtattta
    26161 gttctaaatt ttttaagaac tcaaatatat tatatttttc tatatttttt ggtggtttta
    26221 ctccaaattc aactgaaaga ggccagttta tataagtttt ttcttttgat atgttgaatt
    26281 ttttcataaa tttataattt tgctttttaa tatttttatt ttcatcatat tttacataaa
    26341 ctatataaga atccttttct ttaggctgaa aataatcgtt tacaaatccc tccagagcag
    26401 tttctgggtt tgaaagactt gataaaatac cacataactt atagatttta ccatctaaat
    26461 tgatttctgt tccaatttct ttgtttacat ttaaattgtt tagagaatat gtgtcaagta
    26521 ctatctctcc atctttacta ggaagagttc cttttaataa ttttctacct gtcatgtcta
    26581 aatatgaaga atcagcattt aaaaattcta taataaaagg ttcatctatc tgatttttag
    26641 gttttgtaac gccaagtcta tctacagtgt attcctttga ttttttattt tttttaattt
    26701 tattaagttg ttccttatct actttataga aataatgata atctccacta tcttccctta
    26761 tcttttcaat tctacttttg tctgcactat gaactaaaga accaattccc gtcaataatg
    26821 ctactgatag tactatactt aaaactatag atatagattg ttttttataa tgctttatat
    26881 attgaattga aaattttact agagacatca tagtacttca ccaccagtat tcaccacaac
    26941 tccatcttca atatggataa ttgagtcgca tacttgtgct atattttcat tgtgtgttac
    27001 cataagtatc gtttgatgga attttgcact actttctttt aataaacata ccacttccat
    27061 tgtagtttta ctatcgagat ttcctgttgg ttcatctgct aatataagcg caggtttatt
    27121 tgccaaagct ctagcaattg ctaccctctg ttgttgacca ccagatagtt cgcttggaaa
    27181 ttttaaacgc ttatcccaaa ttcccaatgt cttcatcagc atttcaatat attcatggtc
    27241 tatatggttt cctttatcta atgtaacagg aagagctata ttatcataag tgtttaatac
    27301 gggcattaaa ctgtagtttt gaaatataaa tcctatattt cttcttctaa acactgtcaa
    27361 atcttttttt cctattttag atatgttctt tcctttaatt attacatcac ctgaggttgg
    27421 attatctaat ccacctataa tatttaacaa tgtgcttttt ccagaaccag aagttccaac
    27481 tatagcagta aatgtatctc gttcaatttc tatacttact ccatttaagg cttttacttt
    27541 agtctcacct tttccatagt gttttactaa attattcgtc actaatatac tcatttaatt
    27601 tctcctttca atacagatat atgttctata aaatattgta agtgacgata attacactca
    27661 tcctacaaaa ttagtttaaa ctcttacaat tctgtaagaa gatactaaaa caacatccac
    27721 ttcctagttt agattctact ataatgtttc cattttcttt ttctaaaatt aatcttgtaa
    27781 gatatagacc aatccctgtg ccattttggt tttcaacatt tttacttctg taaaatcttt
    27841 taaaaatatc atttagttct ctactatcaa tacctattcc ctcatcttta atagtaatct
    27901 tcgtatatag ttccattttt attacagaaa tagtaatcgt gctatctgat ggtgagtatt
    27961 ttattgcatt ttctaaaaca tttacaattg cttctatggt ccacttttta ttatgaacta
    28021 attttatgtc tgaaaatggt tcgagattta tccttatatt tttagcttct gctttattaa
    28081 agatagtact gatactttga ataagagtat ctttaattag tagttcttct acttcaaatt
    28141 ctataactcc atcctctaat ttagtcattt taaataagga ttgtaacagc cagtcaatct
    28201 taaaagtttc atttttcatc ttttgtaaaa actccttctt ctgtaatgtg gaaatattta
    28261 tattctccag aagttcacaa taaatggtta tatttgaaag tggagttttt aattgatgtg
    28321 acatatttga tattaaacct ttaatcgctt ctttttctga ctttgactgt tctacctcta
    28381 tttctatcat atccttaatt ttttttactt ggtgagaaat ttttgacgct cttgtatcct
    28441 ttgtatctgg ataagtaact tctttacctt ccaatatttg atttaacatc aaatttaatt
    28501 catcatatct ttttcgattt attttagtag atactataaa tataacaact gtacatatta
    28561 taaaggctaa tataaacata ttattctacc tcattccata aataacccat tccataagct
    28621 gtttttatat attttggatt agatgggtca tcctctactt ttactctaag tcttcttata
    28681 tttacagata caatattatc atcaacataa ttttcttcgc tgtcccaaat atggtgtaaa
    28741 atttgttcct ttaataatat ttgaccttta ttttcaatta gataacttag aagcttatat
    28801 tctacagagc taagttccaa acattcttta tttttatata cttttagtaa cttttggtcc
    28861 aattttattc cattggaata tattacttta ttattcgatt tttttcttat tgctactttg
    28921 actcttgcct ttaatacaga taaagaaaat ggttttgtaa tgtaatcatc aagccctatt
    28981 tctaatcctt ttacttcatc aatttctgag tctcttgcag taagcattat aattggtata
    29041 tctgactttt cccttattat ttgacataca tcaaatccat ctccatctgg aagattgcag
    29101 tctaagatta taaaatctat atttttacta ttaaatatat ttaatccttc ttgttttgta
    29161 taagccttaa atacattaaa tccatcattc tcaaatgcat aaaaaagacc ttcatttaag
    29221 tttatatcat cttcaataat taataagttc aattttactc tcctttagat tttttagata
    29281 aataattatt ttcttgcatt atcatactaa aaatatttta attttattta tttcactaaa
    29341 aagattgtat ttaaatttgt tttcaaaata aaaacattat aatcaagcat atagttatat
    29401 atttcccaat ttaattacgc ttaaattgaa aatataactt tattcttata taattaaatt
    29461 atacaacaat aaagttatat tttttaaata tatttttact agggggaata gaattgaaaa
    29521 gggttgatga gagaaatact atgtttgcaa gagcaaatta caaaaaagat agtactgctt
    29581 ataatgacta ttataagaaa aatcctgaca aaaaagaaat tgatgatagt ataagaaata
    29641 gaccaaattt atgtagtgaa ggaacaatga cttacaatga gttaaactct ccaatggcaa
    29701 gtagtgcatt cgatttttta tctgatatca aatctttatg cgaaggaaaa gtttctgata
    29761 ctaaagtaga tgtagatgca aagtcaatga caaaaaaaat taagggtctt gcaaagcagt
    29821 atggagcttc tgtagttggt ataactaaat taaaagacta tcatatttat acaaataggg
    29881 gtagacatga agaaaattac ggtgaagaaa ttaatctaac tcataagtat gcaattgtat
    29941 ttggatgtga gatggataaa gaaatgataa atagagctcc aatgatatgt gaagttatag
    30001 aaacatcaaa gtgctatgtg gatgcatcta tcgtcggcat gattttaagt tactatataa
    30061 gaaatttagg atatgatgct agaaatcata tggatgctaa ttatttagta atgcctgttt
    30121 ttatagctcg tgatgctggt ttaggagata taggaagaaa tgctatactt acaaataaag
    30181 attatggctc tagacttaga ttaggtgttg ttactacaga tattcctctt ttagaagatg
    30241 aatatgttga ttttggacta gaagattttt gtaaggtctg taaaaaatgc tcttttaact
    30301 gcccttctca ttcgctttct aatgatatta aaattggtga tgatggaaaa tataattggg
    30361 taattgaaca tgagacatgc tatattaaat ggagatattt aggtacagat tgtggtatgt
    30421 gtatatctgc ttgcccattt agccaaaatc tcgaaactat aaagaatact acttcattta
    30481 agggtaataa tgagcttatt caaaaagctt tagatgagta tacttcaaaa tttggtaaaa
    30541 gaatatttat acctggaaat ccatcttggt tgaaataatt ttatttaata aagaatctgt
    30601 actagaattt atcataggac aggttcttta ttaaatatat aaatttttaa atactttttc
    30661 tcgtttataa caattcagca atgtttttag tctataattg acattataaa atattaaact
    30721 aaaaggagta actgtatgga aaaaatatta gtagtagaag atgattctat attaaataaa
    30781 actctatcat ataacctaat tgaagatgga tatataatta cttcaaaatt tacagctaaa
    30841 tctgcactaa agtctatttt tgaatgtgaa tttgatttaa taattttgga tattaattta
    30901 ccagataaaa gtgggtttga attatgtaat gaaataaagg aaaattacaa tataccaata
    30961 atttttttaa ctgctaatga tatggaatgt gatatgatta aaggctatga acttggagct
    31021 ttggattata taacaaaacc atttaatatc aatatattta aacaaaaaat taaagctttt
    31081 ttaaatcatc ttacaataaa aacaaaacaa gattactata gggatggata tcttgaaatt
    31141 aacttttctg agttatctgc caatataaat ggaaatcaaa ttatatttac accattagaa
    31201 tatcgtactt taaaactgct tactgaaaat cctaaaagta ttttaacaag gaaagtactt
    31261 ttagaaaaac tctgggatat agatgctaac tttgtagatg aacatacttt aacatctgtt
    31321 ataagtagga ttagaagcaa aattgaaaaa gacaatctta agtatatcaa aacggtatat
    31381 ggtatgggat atatgtggtt aggtgaaaga aatgaatttt aaaaaatttt cttttagttc
    31441 tatttattta ataattgctg ttggtataat ttttacagct attttattta gtatctcaat
    31501 ttatctaata actgatgatt taaaactaat tgctctatta ttaatatata caatagtact
    31561 ttgtggattt atagcattaa taatattttt acttcataaa aaaatagtta tattttgttc
    31621 tagaatatgt cacactttag atagtatgat gaatagtgaa gataatattg aaattgaatt
    31681 agaagaagaa actattttat cacgtatcaa tcacagatta attagaatgt atgaagttat
    31741 tcaagaaaat cgcaatagta ttgctataga gaaagctgat ttacaagaac tagtttctga
    31801 tatatctcat caaattaaaa ctcctattgc aaatttaaaa atgattaata ctactctact
    31861 aaatcaaaca tttaaaacag aaattcaaaa ggatttttta atggatatgg aaaaccaatt
    31921 agataaatta gattttttaa tgcaatctat ggtaaaaacc tccagactag agacaggtat
    31981 aatcacacta tctaaaaaga aaaattcaat ttatgaaaca attgctacag cacttagtgg
    32041 aattttgttt gatgctgaaa aaaagaatat tgaagttaca gttgattgtg acccaaattt
    32101 atatataatt catgacaaga aatggacttc tgaagctatt tttaatatat tagataatgc
    32161 tgtaaaatac acaagttcta atggtaagat acgtgtagct acagaatgtt gggttatgta
    32221 tacaaaaata gatattattg ataatggaaa aggaatatca gaaagtcgcc aagcagaaat
    32281 ctttaaaaga ttttatcgtg aagaagatgt ccatgacata gaaggtattg gaataggtct
    32341 ctatctttca agaaaaatta tatctcttca aggtggctat attaaagtta catcagatat
    32401 tggaaaaggg tcgacttttt ctattttctt acctaataaa taaattttta aatatttcat
    32461 ttgtaatgat tctgcaacat tttggtttta tactgtattt acaagatgtt aaggaggtat
    32521 tattatgagt attttacaag taattaattt aaagaaatat tatggacaaa gttcaaacat
    32581 tacaaaagct cttgatggtg taactctatc tattgaaaaa ggcgaattta tttctattgt
    32641 aggtacatct ggtagtggca aatccacatt attaaatatg atgggaggat tagataatcc
    32701 tacatctgga aaggttatta taaagggtaa ggaattatct actatgtctg atgaacagct
    32761 tacaatcttt agacgcagaa atataggttt tatatttcaa aattataatc ttgttccttt
    32821 acttaatgta tatgaaaata ttatcttacc aataggattg gatggagaga ccatagataa
    32881 ggattttata aatgaaatta taaatatgct ttctcttgaa gaaaaattat atgatatacc
    32941 aagtaatctt tctggtggtc aacagcaacg tgttgctata gctagagcac ttgtaacaaa
    33001 accagctatt atactagctg atgaaccaac aggtaacctt gatagtagaa ctagttcaga
    33061 tgtactgagt ttacttaaag tgacaagtaa aaaattcaac caaacaattg taatgattac
    33121 acacaataat gaaattgcac aactaactga tagaattatt cgtattgaag atggaaaaat
    33181 tgttaactag gaggtgtagt atatgtttag aaataataat cgcacattta taagaaaaat
    33241 agctttaaat gatttaaaaa ttaataaact taaaacatat ctttcaggta tcattataat
    33301 gatttctaca tgtttattat taacagtgac cttagtttca tataatgcct ctgtagattt
    33361 agttaatgca tctccatatc atgctatata taaatctgta gatgaaaaag caaaaaatat
    33421 actatataaa gataaaaact ttagtggcgt aggaacttac aaattaattg gaagtaataa
    33481 aaagacagat tgtattatgt cgattgttta tgcagatgat acagcaatta aacttatgaa
    33541 ttttcaacca ttgaaaggca aactacctac aaataaaaat gaaattgcta tttcagaaaa
    33601 atatttacag gaatttggtt tagataaaga tattggagat agcattaaat taaattatta
    33661 tgatgatatt acgaataaag aagttcaatg tgattttatt attgttggat ttctagaaaa
    33721 ttattatcaa gataatgcca agcaatacta tacagtcgtt tcaaatgatt attttaagaa
    33781 tatagcttct acacctttac aaaataaaag taatactttt gacgagtcaa gacctgatac
    33841 tgtagatgta ttagttaaac tatcagaaga aaatacagag aaagattcag cttcaattaa
    33901 gacacagtta aataaaattg gtttatcttt aggtattaag aaatataata tatacttaaa
    33961 tgataactat attgaatcaa atttaattga tggtgagcag attattacag ttttagtagt
    34021 tggaatagtt gttctttttt ctagcgtttt tgttatatat agtatttttt atatttcagt
    34081 tgttaattca gttcagatgt atgctaagtt gaaatcgtta ggtatgactt cttttcagtt
    34141 aaaaaaaatt atttctctac aaggaaatat tttgtcaatt atttttattc cattaggggt
    34201 tatagcttct tgtataattg cttacataat tcaaccatta gcatggcaaa tgatagctga
    34261 tttatttata attttaattt tatcactggt aatgttttta actgttagaa tttctttatt
    34321 taaaccagct agaattattt ctaaaatatc tgctattgaa gccatgcaat atacagaaac
    34381 taaatttaga aaaaaagcaa aaaattttag ttatataaat ataaaaaatc tagcattaaa
    34441 gaatatcgag tcaaatagaa agaaaaattt gattgcatta atatcgctaa gcattagtgg
    34501 agttttattt atttcaatcg ctaatcttgc taattcaata gatttcaaaa aacaactatc
    34561 tcaacaattt gtctataacg aagattatat aattagcatt aagagtgata acttatatga
    34621 gcatattaca gaaatccaac aaaataatcc actaacagat tctcttaaga atgaaattaa
    34681 atctattcat ggagtaaaaa aattaattga atccaaatca attaaaagta atattataga
    34741 accttacatc aaagatagag acgaaaaaca atttattact ctaattaaag gtattacgcc
    34801 tgaactttca aaatcacttg aagaatatat tgtcagtgga aaaatagact atacacattt
    34861 agattcagat agtttaatta taaataaata tagaacaaaa tattatggat taaatttaaa
    34921 agttggagat acagttacat ttaatatctt tagtggaaat gatattatta aaaagaaaat
    34981 gaaaattata ggaatcattg acaattccaa tacaggaggt atgttttttg cttcagacaa
    35041 aactttagaa tctctaacac catataatac aaatttagat ttttctattt taatagataa
    35101 aaagcattct aaagaagttg aagaaaagtt gttaagtata attcagaaaa attcaaattt
    35161 aatgctttat tcatatgaag atgattatag aatgattact agagcatttc aatatataat
    35221 tatagcttca tacatttttg ttggtttaat ttcttgcttt ggtatactaa atatgaccaa
    35281 taccctaata aacagcgttt taattagaaa aaaagagttt gcattacttc aagccgttgg
    35341 aatgacaaga aagcaactgc gaaatatgct ttatagagaa ggtctaaaca taagtatcaa
    35401 agctatatgt acttcaagta tactaggtta ttttggttca aatctacttt gcacttttat
    35461 aaaagatgta attagattag actttattaa ttttaaattt tctatattta ctattttaat
    35521 tttttcattc gtattaattg gtatacaagt tttagtgact gaattacttg taagaaatat
    35581 agaaaaaaaa tcacttacag aacgcttacg ttctcagtaa gaatgtatat aaaaaatagc
    35641 cattttttat acaagtttta acataatttt ttaaaagcta aaaggataga aaattttgac
    35701 tttacataat tttctatccc tatacttgtt tttattttat acagatacac ttacttatat
    35761 ctccgagcaa catcaccaga gacataaaca agcatattcc ttattttcta aatatgtcaa
    35821 ttgcatgact tgtaacccct actaaatctt cacgttcaca agtagcaagg tgatatttta
    35881 aatataatgc aaatgcatca tcatccatag agtctactgc ttcacgcata tacaaagcca
    35941 aaccatctga tgcgacataa tgcagtctag ttacattaaa tgcagacatc aaatcatcaa
    36001 tgttttcttt acgtacaagc tcaaacaaat cctttggttc agacttagca gcaaatgtct
    36061 gtggatcaat caaacctttt tcaatatatt cagatacatt aatatttcct ctatggaacc
    36121 cctcgtcaat aagacagcca tcagatataa catatgcagc aaaaaccaca ccaccaggtt
    36181 ttgttacacg aattgcctca tgtaaagctt gttgtttatc ttctttgtta taaagatgat
    36241 atagtggtcc taacagtaac gtaatatcat atttattatc tgggaaaaca gataaatcca
    36301 tagcattacc ctgagtaatg cttatatttt catttgattg agtattttta tggaaaacct
    36361 ctatattatg ttcgactaat tccactgcgt caacatcata tccctgacgt gccagtgtgt
    36421 gagaataacg accagttgca gcgccaattt caagcacaca atcacctgat tttatgtact
    36481 tatcaatgta atgcatagta gtgagaaatt ccaccattcc atgttttaat gcaagtcgac
    36541 tatcttcatc ataagtatta taaaagtcaa ttaagtattg ttttgtttcc ataatttcac
    36601 ctcttctata ttagttaata aacatcaaat agacaaattc caatctgatt tttcttgtct
    36661 tataaaagct gttaatatat ttttcaacat tttttcagca ctatatctat ctaatgttaa
    36721 tttttttgtt gctgttaaga cagctatgcc atgagtataa agaaataaat ctaaaaatat
    36781 tacttttgca cattccaaat ctattccaat gctttccgaa aaaagtcttg cctttttttc
    36841 attgtcaatt tctttataaa aatcattcgc ctctttcatc atcaaatcta aatcatttat
    36901 aaataacaac ttaaataaat acgtttcttc ttgagcaaat tcaatgtatg aaagcggaag
    36961 cagtaaataa gggcttacac ttttagaatt acgataatta acaacatact gattataata
    37021 ttcatatgca aaagcaagaa attcattttt taattcattc atgttttcat aacaagtaaa
    37081 aattggtcga gtggaacact gtaatttact tgcaatactc ctcgcattta cagcttcaaa
    37141 tcccacttct cttgtaatat ccaagactgt attcaaaatc atttcttttg ttatctttac
    37201 tttaggtggc atatatgttc acctctccat taaattttta taaaacacat gtgttatata
    37261 actatagtaa catatcaaga aattatgtca agaaaaagct ttgcattttt ttgcaattcc
    37321 taaaacttat aaataaaaca tgagttgcaa ttaatctatt aatagataac agcaactcag
    37381 attttccttt aaatactctt ctttttgtct aatatttcat atacacaatc ttctattgaa
    37441 ttaatatctt tttcttttct ttttttagaa tcaattaaaa tgaaatacag tataaaacct
    37501 acagcaagtc cccatgttcc gcctttagca gcaattacac ctcccataag tccaatcaat
    37561 cccttctcaa cacctgtatt acatagctcc attgatagct ttgtacataa atatccttga
    37621 actaaaagtg ttactgacaa tgctaatggt ataagaggtg tagcaatctg cgcaattgga
    37681 taaaaacaga taaaaattgc tgaaacccat actaatgtac ccatgccact aaaaacagac
    37741 tgcatggctt cctttccaac cttgtacctt tgagcaacac ttcctgtaag tgtcgcagct
    37801 aatggaccgc acattggtat atatggagaa aagattgcca taaaaatatt tcttatccca
    37861 cttataacat tagacctatt tgaattgaaa tctatatatt catcttttct attttcactt
    37921 tcagaaagta ctaagttttc acctgttata aaatcaccaa aggcaattat gtatatcata
    37981 aaagccattg gtatcccttg tataaataac attacacttg gaaaacctat tccaaaagga
    38041 gatactgtat ttatcagatt tttaaaatct actatgtaaa ctaaattgtc aaaactaaat
    38101 ctaggaattt ctatttcttt actaaatata cctactatta aactcactaa aatagctgat
    38161 actacaccat attctcctaa atgaaataaa aatttattct taacttttag agtatcaaat
    38221 ctttcagaaa acattacaaa acaagcaacc aaaattccta ctgttataga tataggataa
    38281 gaatcaaatc tccctttagg attaaactct gatattatag tcgatatacc gacacccatt
    38341 agtattccac ctttaataga atttggtatc ttactaacta actttccagc tacaccagtt
    38401 atacccatta aaataaatat tattcctaaa tctatttgta aagctgttaa tgcttgtatt
    38461 ctttcaatgc ccatgctgaa ctttaacaaa tatgcagttg taagagttat agaagctgta
    38521 atccagccag gtacaactgg gtctcccata aaaacatgca aacagtataa tattgagtta
    38581 atgatagcca tactccatgc cacttcatag ggtaatccca aaatttgtgt actaaggcca
    38641 attgtgccta aacttgtcat acttgatatt ataccttgaa tagcttctgg tttctcaaat
    38701 ctgtaatgta taaatggaat tcttatctta aacattccaa attttatatg aggttgttcc
    38761 cttctagcga gtctttccat aacttcactc cacctttcaa taactcttat aagtttacaa
    38821 ctttataatt gtttataaat tatcaatgtc attaaaatta gtatataaca tgcaaattat
    38881 ataaacaata aattaaatct ataaaattga tagattttgt gcatatatag aaagaccgct
    38941 tcatttttag ttacttaaat cacttaatag tagttattta aatcacttga taaaggtttt
    39001 ttataaaatt caatagttct attccatgat tcaaactgtc cttttgaatt tccttctaca
    39061 gttccacctg aagaaaaaat ccctccactc atactcatac ataaagtctc ctttagaggc
    39121 ataatatatg gaattggtaa cggatgaccc atattattgt atgttaataa ttctacgtta
    39181 tatggataat tattttcttc aaagatttct aatgccttta aacaagcttc ataactattc
    39241 catatgttat cttcttttcc agcaatcata agtatatctg ctttggaatt ttctattttt
    39301 attctagcct cttttctatt ctctgctctt ttaagacttt tcttataagt actattaaat
    39361 ccaaatggaa taccttttga tatatttttt ttctgttctt caaagaatat atcattgtct
    39421 actttgatat atggaaatgg ctctccttta tatgaccatg atgaagtatc atttccactc
    39481 atcaatccat taagagcttg gaaacaataa atgtgaggtt gtgatactac aaccttttta
    39541 atttgttcat atcttgaagc aagtaaaaag gccaattctc caccctttga tgtcccatgt
    39601 acaaatattt ctttagtact ggtaatttca ttttcactaa tccactcaaa tactttttca
    39661 aaatattcta atggaacctc ttctaattta tctggtaacc cttctaaagc aaagtatgca
    39721 actgttagta cattgaatcc tctagatgca agtggagctg ctaaaaggtc tagagcgtct
    39781 aaatttccat cagaaccacc tagcattaaa attgttttat tattagaatt ttgtttataa
    39841 aataattttc ctataaactc atcagatata tattttgtgg taatatcttc tgatttaaat
    39901 attctcttta attcaacttc ttttctttca atagaagttt caaatatcat attcataatt
    39961 attggtttat caatagatat attttctgca aaatttttac cttcagtttt agatttttcc
    40021 agcgaataaa ttaaccccat acaattatca gccttataag taccttctat aggttcaact
    40081 aaatctaaat ctacttctcc tttttcattt gaacaaaaca ctccataaga tgaaaattct
    40141 tctcccttgc accatggaaa ttccattttc acacttattt taagtttagt atttggaatt
    40201 aagtccgaaa ccataatctt aagtctttca tccacaaatg attctacatt gtatacattt
    40261 actttcataa tctatttctc cttataataa ttatttatgt tttatataaa acagctttat
    40321 ctaatacaac ataaataaat ttttaaatta agacaccata ttgactattt cattttatca
    40381 acatattcta tttttgtcaa catattgtct tttttagcaa tttaaaatta tgtagtctat
    40441 atgaaatcca gttgattttt ggctaattaa agataggaat gctattaggt ttagaatata
    40501 tatattcaaa ataaaaaacc actcatttta agaatggttt ttaatataaa ctatttgtta
    40561 ttaataagga tattttttta tttaaattta tataatcgaa catctaatat ttaatgttta
    40621 ataaaattat gcatttgcaa aagtatatat ggcattaact cttgctacat ataaattctc
    40681 atcactacat ccaatatatc tagcatattt ttcaatcttt tcttttgcat tttcaacatc
    40741 attttcatcg ctctccatta actcatttat ataaatatcc attaatgacg ctttatattc
    40801 tcttatatca tagcctaaaa attcagtcaa tctctcaatt tccttactaa aatctccatc
    40861 ttcttttacc aaaaattcgt atacatcttc cctatatgga tttaaagtta ttatttgttt
    40921 tactactttg cattcgtcag ccttcccaat aaaaccatct ttataattat taaataaggc
    40981 attagatttt tttatagatt cttcatcaat ataagaatgt accgcagtta tacgattttc
    41041 tataagggca tctacaacag cataatgaat tttaaaacaa tcattgtata aataattgct
    41101 cattttcttt attgtcgatt ctatttctaa gaagctcgcg tggccatttt ttttattttt
    41161 atttggtata ataaaagttt tattcaagtt attaaaaagt ctcttaaaat ctaaatattt
    41221 ttcacaataa acacttttaa aggtattgat atctatagtt ataattccaa aacttactat
    41281 agtctcaatt cctttcttaa aagactcttc tatatatttt tctcctaaac ttaaacctct
    41341 atcagatatt tgctttagat tttgaaaact atttaaacaa tctctctcaa agtttttaga
    41401 tatttccaaa gcatataatt taaagatttt ccttatctta ttataattat tttttgtatc
    41461 atcaaatctg ataatagctc catttacatc aaaagtaatc attgagtttt aacctcccca
    41521 ttagtttcat caataaaata aatttaatat aaactttata atcataatat taaacttatt
    41581 tatactataa ttattaattt gatgataaaa gctattttcc ttctaaataa aaaatttgtc
    41641 taaagtttct aaaaaattta gacaaatctt aggttattac tctttaaatt atttttaaat
    41701 tatttttaaa ttattccaaa tacgccaaat aatattggta taaatatagc tggtaacaaa
    41761 ttggctactt ttatattcgt taatttaagt acatttagac caagtcctat aattaataaa
    41821 cttccagctg ctgtcatttc tgtgactaca ggatcactta aaaatccatt taaaaatgat
    41881 gctcccacac atataatacc ttcatacaaa aatacagata ttgcagaaaa cattacacct
    41941 atacctaaag aagcagtaaa tataattgaa gctattccat ctataacaga ttttacaaat
    42001 aaagtatcat gacttcctgt aagaccactt tctaatgaac caactactgc catggctcct
    42061 atacagaata ataaactaga tgatataaaa ccctctgcta tagaatcttt tttatttcct
    42121 ttagaaaatt tactttgtaa aaaagcaccc aacttattta cccatttgtc aatatctatt
    42181 aattctccta tcagagcacc tacagctacg gatattattg tcactaatgt gttttttcct
    42241 tctagtgctc ctgatatacc aatgtacaat acacataagg caattccatt cattatagta
    42301 tcgcttactc tttgaggtaa tcctcctttt attataagac ctactataca tccacctgca
    42361 attgctagac tatttgctat tactccaaac attttttctc ccctcgatta gctaattgaa
    42421 atttatccta ttattaaaac ctaaacatct atactttgta ttggatattt taaatattcc
    42481 aaaaattctt caaaaccttt tctagatata tttacagttt tagtatttat gttaggatga
    42541 aaatttaagt aatcttcttt aattacatct ttatctatgt aaagctcaac ttttctttct
    42601 gtatcattta ttaatgaaaa aggatttaca cttccaggaa gaagtttaag tacattgtat
    42661 agtctttcag gtgaagcaaa cgataatcta gaacttccta tcttatcttt aagtgagttt
    42721 aaatcaactt gtttttctcc tttaactaca ataagataat acttatttcc tttggcattt
    42781 cttaaaaata aatttttaca ttgacacccc tttgttattc catctaaaat gactaattgc
    42841 tctgctgtat atacagcttc atgctctact acatcatatt caacatttaa tttatccaaa
    42901 atatcatata ttttagtttc attcatgcta gattcatttt catcttgttc ttttttatta
    42961 tttaaatcac tcatactttt ctccttaaac acttcatcat aatatcattg tgaggtatta
    43021 ttctactata gctattgctc ttactggagc tccatcagca tcattatact taaatggtgc
    43081 tgctataaat agaaattttt ctggaacatt ttctaaattt gtaagatttt ctatgattat
    43141 ttttcctttt tcaaataaaa tatgatgcac ttcaaatact gatgtgtcat atctatccac
    43201 tgatagcata tctattccta tgccttttat attagtatta gctatatagt ttgcagcttc
    43261 ttttgttaat gtaggataac ctacatagta ctgcttttta tcccaaaatt ttgaccaacc
    43321 tgatttaaat attataaaat cactattttt tatttcatct tcatattttt ttagatattc
    43381 taattctata ttctcatttc ccttttcaag ttccaataca taagctttcc caacaaaatt
    43441 ttctatatcc aacttatcta gggtctcacc tttagtatac atatgtcttg gtgaatccat
    43501 atgagttcca ttgtgagaaa atacactaat taatgtttct tgataaccat tttcttcaat
    43561 aatagctact ttcttgatat ctggtctatt aggctctgca tatacaggca tgtcattatg
    43621 tgtcacatga gttaaatcaa aaaccttcat ctttatatac cccctatgtt tttattttct
    43681 attatatacc taaattcatt ttatttctat cagattttat tccattatat accatactac
    43741 tatatatttt ttataaacct caagggattt accgaaaaat cttggaaaga attatcagta
    43801 aatataacat cttcacccaa aaaacgttta taccttcctt cttctttctt taaagtaatt
    43861 ctctgttttt gtgaatgatt attaaaaacc attatcttaa gaggaggttt ttttctatta
    43921 gtctgcactc tttttaacat gtattttatg tgcatatcag cttcaggaaa tgaatatcca
    43981 caaaatatta aaaggtcagt atctcttaaa gttttttctg tttcattcca aacatcactt
    44041 aaaaatatgt ttgacatatt cttaaaatat gttggtggca ctataatagg ttctgttaac
    44101 tcaccacact ctaaacattt tgtctctgaa ctattttcaa taagcttcat gacaccacct
    44161 tcatgtggtg taagagttac actattacaa atggggcaat aaagccaatt caatgaccca
    44221 tgtatcttat ataatttgat taatggttct tttggttttc tccaatttaa ctcctcttta
    44281 aaatttgtaa actccacacc ataatcaagc ataattggca aatcattctt atctcttaat
    44341 ccagtaactg tattgtctat atgtatatca taatttgcac taataaaagt agtatcaaga
    44401 agcaaatcat tttccaaaag attttcaact aaaagtttat gatatttatt atctgttcta
    44461 ggtgtattat gaattgcttc agccattagc agtattaaat attgtcttaa aaaacgtata
    44521 ctatcacttc tgttatttag attttctagt ccaaaatttc taaatgcttc ttttctttgt
    44581 tctgctaagt ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc attatctata
    44641 ttatctttta ctatatcaat attgaacatc tgtttaaaga aaatatacaa ttctctattc
    44701 attttggtat tataatttgt gggatttagt tttttaaaat attcgctaaa aagttcattt
    44761 tgaataggta gattctctgc tgctgaagct cctgctccta aaaatattgc tattttcata
    44821 tagttcctcc taatataaat attactccta tttttatatc cattaaatat ccatatcatt
    44881 tattttatga tacaacaata aagctgtctt ttactagaaa aaatcctatt acaagacagc
    44941 cttatattat attaaatctt taataattcg taaatagaaa gagcatatat ttgagtagct
    45001 tttataatat cctctactat taagtattca ttagcttggt gttctgtttc tggttttcct
    45061 ggaaaaatag ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt tgctcctcca
    45121 gatgaaagtg gtgttccatc aagaccagtt tcttcttcat atacttttct aagtgtctta
    45181 acaagaagag tatcttctgg aacatagatt gaatccaaga aatcatattc ttcataattt
    45241 aaattgtatt tatctgtcat tttctttaac tcttttacaa aatcatcctt tttgtatgtt
    45301 actggaatcc ttacatctat tcctgcaaat tctttttcgt tatcaatagt tactctacct
    45361 atatttactg ttagtttacc tgatacatca tccttgcagt taggaattat attgtttcca
    45421 ttagcatctt caccaataac ttctgctaaa aacttaatta tattagaatc tatacctata
    45481 ttattaagag ctatacaaag tctagccact gcatttatac cagtatcact tgctgctgaa
    45541 tggacactct ttccaattat acagatttta ttaccttcaa cagtatactc aaaatttaat
    45601 ttattaagtt ctttttttaa tttatcagag tattttccaa tatagattgc tttacctgga
    45661 actgcattta aagcctttcc aactgacaac tctatatcac ttttgctatc acaagtcaaa
    45721 tgaacttgta aaagaccttt ctctgcattt gttattggga atctagaatc tggagtaaat
    45781 ccatagtttg gtatttcttc attattttct ttatatttat ttatacatct ccaaagattt
    45841 tcttcatcag ccccaaaaat gaatcttatc ctcttattaa agtctacatt taaatccata
    45901 agagctttca ctgcataaat tgctgatatt gtaggtcctt tatcatcttg tgttcctctt
    45961 ccatacaatt taccatcttc taaaacagct tcaaatggag ggtaattcca gctttctaaa
    46021 tcaccttctg gaaccacatc aacgtgacca agtataccta tcattttttc accttgtcct
    46081 atttctgcat atccataata tccatctttg tatactgtct taaatcctaa agatttacat
    46141 aaatctaata tacctcttaa agctttatct acattttcac caaatgggca attttcagtt
    46201 gcttcagata taacactagg tatttttaca ctttcttgta ttgaagaaat catttcatct
    46261 tgtagagaat ttactttttc tttaatttgc tctttcatat tatcaccctt caattttcat
    46321 cataatatac taataaatat tttgaccttt taaaaaaaaa atatgcttat tttttcagtt
    46381 tttttataaa ttcaatcgct tttaaagtcc ccatttttga atggtcaaat gtaagtccac
    46441 cttggaaata aacattataa ggaggtctta ttggagcatc tgcacttaat tctatagaag
    46501 aaccttgtat aaatgctcct gctgccatta tgacttcact ttcgtatcca ggcatgtccc
    46561 aaggaacagg ttttacataa gaatcgactg gtgcagcttc ttgtatacct tcacaaaaac
    46621 ttattacttc atcagcatta ttaagtctaa tacattgaat aatatcactt cttaagtcat
    46681 catattttgg taatacatca tatcctaatt tttcaaaagc tcttgagcaa aagatagctc
    46741 ccattacagc ctgagatact atataaggcg ccaagaaaaa accctgaagt acatttctag
    46801 ttgttccaaa agtaagacca cattcttttc ctattccagg agaagtcatt ctataagata
    46861 taagctcaat caaatctttt ctaccagcta tatatccacc tgtcaatgca agcccaccac
    46921 caggattttt tattaaagag ccagccataa catctgctcc aacatcagta ggctctttgg
    46981 tatctaaaaa ctcaccataa caattatcaa ccattactat agcttctggt ttaacagact
    47041 ttattacttc tatagcttct tttatatcac taattgaaag tgattttctc caagaatatc
    47101 cttttgacct ttgtatcata acgagtttag ttctatcatt tattttgttt ttaattcctt
    47161 ctaaatctaa gtttccatcc tctaaaaaat ctacatcatc atatgtaaca ccatactctt
    47221 tcaatgaacc tttttcttct cttatgccta taactccctc taaagtatca tatggtctac
    47281 cagtaacaga taaaatttca tctcctggtc taactatacc ttgtatacat aaagtaagtg
    47341 catgtgtacc atttacaatt attggtctta caagagcatc ttctgtatta aatactttag
    47401 aataaatttc ctctattttt tcacgaccaa tatcattata tccatatcct gtagtccagt
    47461 taaagtgcat atcacttagt ttagattctt gcatagcttt taaaacttta tattgattat
    47521 attctcttat ttcttttatt tcttcaaact tatcttttat ttcttccatt atttcttgcg
    47581 atagtttaaa agtatcatca tctattccat agtagtcttt taataattct tttgtctcat
    47641 taagcatttt atcacctgtc tctttattat tttatttaaa catgtaaagg agactatttt
    47701 actaaaacaa ataaattata gtttataagt tagtataagt ctcctctcaa tcatcttaaa
    47761 ttaaattaat tatctatctg ttgttattat tattgttgtt ttggttttga tttgttagat
    47821 taatatactt tccaggttgt atagttgata cagcatgttt ataaatcatg ttttgttgtc
    47881 tattatcccc ttctattaat attatataac tatcaaaccc ctttacaagc cctttaactt
    47941 gcactccatt aactaaatat atagtaacag gtatcctttc tttccttgca ttatttaaaa
    48001 acaaatcttg taaatttaaa actgtatttt tcattagatt tgtaccctcc caagattaaa
    48061 ttgaacttct aaaatattcc taccttatat tctatataag ttatgacaaa ttttcaatat
    48121 taagttgtat acttaaaaat ttatatatta ttttatagaa tctttaatat ataagattat
    48181 ttcatttttt aattcgtcta tattttcata ttggtctaaa tcaaaccatt ttgctgtttt
    48241 atatctttta aaccatgtta tctgacgttt tgcatacctt ctagaatctc ttttaattat
    48301 ttcaatagct tcttcgtatg tatattctcc atccagatac tttaatatct ctttataacc
    48361 tataccttgc attgatatca tatctttttt aaatcccata cttaaaagtt tttttacctc
    48421 ttcaaccaat ccatttttaa gcataatatc aactctcata tttattcttt gatacaaatg
    48481 ttctctatct ctatttagaa ctattattat tggttgatat tcttcattaa attttaaatc
    48541 acttgaaaaa tcattcattt tcttaccact tagacaaact tcaagagctc ttatgaccct
    48601 tttagtattg tttttatgga ttctattagc agattcttca tcaagttctt ttaacttatt
    48661 atgcatgtaa tctattccct tttcagcaag ttgtttttga agttcttctc ttaattcatt
    48721 attggcatca gattgtgcaa aatccatatt atatattaaa gagtttaaat aaagtcctgt
    48781 gcctccagtt actaaaacac atttttcctt tttatttatt tcatgtatat atttttttgc
    48841 tctttgttga aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat ctataaggta
    48901 atgcttcact ccttgcatct cttcctctgt aacttttgca cttccaatat ccatatattc
    48961 atatatttgc atagaatcag ctgaaattat ctctgcattc atatcttttg ctaattttat
    49021 tgacaaatct gtttttccaa cagcagttgg acctgtaagt attataagtg gtattttttt
    49081 cataacattc ctttccatat attttaaata tccaaatatt ttacataatt cttttaaaca
    49141 ttttttctat ttctgtctta gatatttcga ccattatagg tcttccatga ggacatgtaa
    49201 atggattctc acacttttct agctgttcca ataaactttt tatttcaata tcgtatattt
    49261 tatcatttgc ttttatagca gacctacatg ccatagatgc aaatctctct ccttttaaat
    49321 cataattact tgtaatttct tctatattat caattatttg aagtataaat ttctcggttt
    49381 caggtactcc aaatatagta ggaacacatc ttaccattat atgattattg ccaaaaattt
    49441 ctagctcaaa accaaatttc ataaataact ccaagttatt ttcaatttga agcatatcta
    49501 catttgatac ttcaataacc actgggtcta aaagtatttg catatttata tcttgtctat
    49561 agaacttctc catatatctt tcatatagta ttctttcatg tgcagcatgt tggtccaaca
    49621 aatacataga atcgtctttg ctcaaaatta tataagtatt aaatacaact ccaattacag
    49681 aatatccgta taaagaaaac ttgcgttttg aactacacaa gtattcttct tcactgtcct
    49741 taatactatc tcctaagtaa taatttcctt catttttaat tccatcaact tgaaactctt
    49801 cttgtatatt atcatttgca ctatcttcac ttagataatt aatgtttttt acatcatctt
    49861 ctacacttgc atttaggact tcacttactg attgaaattc atttattggt ttttcacttg
    49921 ataaagtaac aacttcaatg acttcatcct tacctttagt aatattacta tttttaggag
    49981 tagattctaa taaatcatta tttcttaatt tataatctgt acttttttct ctactgttaa
    50041 tagcgattct aggttgtgtt tttttatcac tataagtagc atattttcca ataagatttg
    50101 agtgtattaa tttaaccttt aaaaaatctc taagttctat atacacttct tgttcttttt
    50161 caaatttaat ttcaagttta ttggggtgta tatttacatc tatacatgat gggtctactt
    50221 ctatatttaa gaagcacact gcatgtttac ctattggaat tatagactta taactttctg
    50281 ttattgcatc tatgattatt ttacttttta caaatctttt atttatatag atatgttgta
    50341 agtttttatt tgaacggtat atattgttat tacctatata tccattcatt ttaaagtgat
    50401 tgcacttaaa ctctacatct attatatttt ccgttatttc ttttccatat atacttctaa
    50461 tagtgtttac aagctttcca tcccctggag tatttagcat ctgtttattg ttatttgtat
    50521 atttaaattg aatgtttgga ttacctattg caagtttatt tatcaaatca cttatattta
    50581 tagtttctgc atgagtagat tttaaaaatt tctgtcttgc tggtgtatta aagaaaatat
    50641 cttttataat gatggtagtt ccattagtag aacctattgg ttctttcgat attatttttc
    50701 caccttcaac atatatttta gttcctatta tttcatcttt agttttagtt gtcatttcta
    50761 attttgatac agctgaaata gatgccaatg cttctcctct aaaaccaaga gagtataaat
    50821 catacaaatc atcaattttt ttaattttac tagtagcatg tctcaaaaat gatttttcaa
    50881 cttcacttga aggtattcct attccattat ctgtaatctt aattagactt tttcctccat
    50941 caattatatc tatggaaatt ttatttgcac ctgcatcaat ggagttttca ataagttctt
    51001 ttactacaga agaaggtctt tctacaactt cacctgctgc tatcttattt atagtcaaat
    51061 catctaggat gttaattata tttttcattt ttacacctct aagattttat ttcttttgct
    51121 tttctttgaa gattataaag agagtttata gcatctaatg gagtcatatt taaaatatcc
    51181 agatttaaaa tttcttctga tagtatatct ctatttacac tatcaaaaga gatttgagtt
    51241 aaggctactt cttttacaga atccacttga ttaattttct ctttatcatc attttgcttt
    51301 attaaagcaa cttcatcatt taatgcttta aatttagaat ttaatttatc ataatccttt
    51361 tttatatgtt tgtagtcttt tactataagt tcatgttcta tttttagaga ttcatattta
    51421 ttcttaaaat tactttgatt taccttaact aattcttcgt ccaaattact attaattata
    51481 ttattgtttt ctttatctcc atttattgca acactgttgt aaacatgatt tttttccaag
    51541 tctttaagta tatattttgc tctttctatg acttcgtctg gtaatttagc taatttagca
    51601 acatatattc cataactctt gtctgctccc tgaggaatta tttttcttaa aaatattatt
    51661 ccttccccat cttctttgac tgctattgaa taatttttaa cctctttaaa ctcttcttct
    51721 aaatcagtaa gttcatgata atgtgttgca aataatgtct tacatctaat atttttttga
    51781 atatattcca ctattgacca tgctaaactt ataccatcat aggtactagt tccacgacct
    51841 atttcatcta atataactaa acttctctct gttgcatttt ttaatataag ggatacttcg
    51901 ttcatctcta ccataaaagt agattgacct tgagataaat cgtcactagc tccaactctt
    51961 gtaaatattc tatctaatat tggaatatca gcagactctg ctggtacaaa tgagcctata
    52021 tgtgccataa gagctattat agcagtttgt ctcatatatg ttgatttacc tgacatatta
    52081 ggaccagtta ttatgttaat aatgttttct ccacgattta aataagtatc atttggaacg
    52141 aaattttcct ctcctactat attttcgaca acaggatgtc taccatttct tatatcaagt
    52201 ttattatttt catttattgc tggttttaca tagttattta tatgtgcaac tgttgcaagg
    52261 gacacaaaaa catctatatt agctatagtc ttagccactt tttgtattct atctatgttt
    52321 ttatatattg tatctcttat ctctacaaat atctcgtatt ctaaagattt aatcttttcc
    52381 tctgcatgta gtatcttttc ttctatttcc tttaattctg gagttatata tctttctgca
    52441 ttactaagtg tttgctttct tatatatgtc tcatctagct ttgcctgttt aaagttagct
    52501 tttgttattt ctatataata tccaaaaact ttattaaagc ctatttttaa tgatttaact
    52561 cctgtttttt ctctttccct gttttctatt tcctttacaa gaaaggctcc attcttagaa
    52621 atctctctta gttccttaag ttcatcacta aaatctgatt taattatgtt tccatctttt
    52681 atggttatcg taggttcttc tagaatagct tcatctatta aattgtatat atcatctaat
    52741 ttatccattt ctgaaacata ctcttttagg atttttgcat ttgataaatt tatagtatct
    52801 tttaaattag gtaacttttc tatcgaattt tttaagtgaa ttaactcttt tggtgttact
    52861 ctctcaaatg ctatctttcc acatattctt tctatatcgt atatattttt aagtatatca
    52921 tttaggtctt ctcttaatat aaagtcatct tttatttctt ctattacatc taatctattt
    52981 tcaattttag atttatttat caaaggttct tctacatatt ttcttaaaag tctacctccc
    53041 attgctgtag aagttttatc aagtacgtgt aataatgaac cttttttctt attccctctt
    53101 atagtttgag taagttctaa attagttcta gtaaacatat ctagaaccat atattctgat
    53161 gaattgtata tatttatatt attaatgtta gatgttattt gtttttgagt attgtatata
    53221 taattcagaa gaattgataa gcttgacttt attagacctt tatcatcaaa ctttagtttt
    53281 tgtagataaa catctggaaa atattctttt aatatattta tatccaaata gttatcacta
    53341 aaactttcat ttatgtatat attacttaca gtagctatat ctctaagatt ttctataaag
    53401 tctaaatcat ttataattat ttcagttgga tggatttttg caatttcttc aattacctta
    53461 tcttcattta aacatgttgc attagtttct cctgtactta tatcaacata agttagacct
    53521 atattagtac catccttata caaagataat aaataattat tctttttatt ttcaagtaaa
    53581 tttccatcta aaactgtacc aggagtgatg actcttataa cctctcttct aacaattcct
    53641 tttgctgtag atgggtcttc catttgttca cctattgcta ctttatatcc attttcaact
    53701 aattttgata tatatgaatt ggctgagtgg aaaggaacac cacacatagg agcacgttct
    53761 tctaaaccac aagcttttcc tgtcaatgct atttcaagag cttttgaagc tactaatgca
    53821 tcttcaaaaa acatttcata aaaatctcca agtctaaaaa acagtataca gtctttatat
    53881 ctatttttta cttcaagata ctgtttcatc ataggagtta atttattcat atctatttgc
    53941 attatcaaac ctccttcttt tctaattaaa tattatatca tagtagatga ttattattaa
    54001 cacaatatat aaaattttga catataacta aaaacaatcc aaaatgtata ctcacatttt
    54061 ggattgctaa gtttaatgct tattaaataa aaatattttt tttacaataa agatattatt
    54121 tcttttttaa gtttaatgat attttcaagg ttatcttcat ttaactttct actatctata
    54181 acaaattctt tctttactat tgatggtttt tttgaaataa cataaattct gtttgatagc
    54241 attatagctt cgtcaatatc atgagttata agaagtattg ttgaatttac ttttttctta
    54301 acatctaaaa gccatttttg catggatgtt ttagttaatg aatcaagtgc cccaaaaggc
    54361 tcatctaaaa gcattatgtc attagaattt ataaaagttc tcaagaagtt agctctctgt
    54421 ctcattcctc cagaaagttc tgaaggatat ttatcttcat atccgcttaa tccaaacacc
    54481 tcaaaataag gttttaccat tgacctagct ttactctttt tttctttttt taaaattagt
    54541 ggtagtgaca cattatctat tatagtttta tatggcaaga gtaaatcttt ttgatacata
    54601 taactatact taccatttat attcactttg ccactatcat aatctgtaag tttagttata
    54661 atattaaaaa tagtactttt tccacatcca cttggcccta atatagaaac aaactcacct
    54721 tcataaacat caatacttat atcttttaaa acttcaatat ctttataagt ttttgataca
    54781 ttttctatag atacttttac ttttttattg tggtaaaaat tcatttgtgt aagcatcact
    54841 tgccttcata tccttatcta ttaatttata ttcttttaag aatgatgtat agttgttcca
    54901 tacactatct ttcatttctc cccatcgtgg tgcatcatct ttgtacttgc ttgctaaata
    54961 ttcttgactc tttaaagcta gtttttcatc tacttcaggt gcatgtttta ctaaaatttt
    55021 tgctgattct tctggatttt ttattgcata ttcataacct tttgttgtag cttttaagaa
    55081 ttttttagca agctctggat tatctttaag cacagtttca ttagaaatta taagtggtgt
    55141 ataataatcc aatctctcat ctaaatcttt tactggtata aagtttaagt catatccaat
    55201 taaatcagct ttaactgcat cccaaccttc aaatatccat gcaaaatcta cagttttcat
    55261 agctgcaaag aaatcatctt gaccagtatt aactatcttt aacttattaa aatcagctcc
    55321 atttttcttc ataactgctt taaaaacagc ttcttctgat ggagagcccc aaccaccata
    55381 agtttttcct tcaaaatctt tagctgttgt tatattcttt tcttttggtg atgcaaaccc
    55441 agaagtatta tgttgtataa cagttgctac agcttttatt ggaagtggat cttcactagt
    55501 ctttgcataa gtaacttgtt cttgatagct tataccaaag tcagcttttc ctgttgctac
    55561 taaagtttct gctccacttt caggtggttg tactatttca acatccaaac cttcttcttt
    55621 atagtaaccc ttatcaagtg ctacaaataa tccagtatga tttgtatttg gtgtccaatc
    55681 aagcaccata gttacttttt tagaagaagt atctttatca ttgtttttgt ctttattgtt
    55741 atttgaagaa catccagtta ttgttgccaa tgtaaaaaca cttactaaac ctaatattcc
    55801 taacttttta aaattcataa ttgcctccct caaatacctt taatctatta atctatgtct
    55861 tataacaact ttttttacag cctgtacaat tcctacaaac attaaactaa atataactac
    55921 taatatagta gatgcaaata ctttatcaag tgcatatgca ctttttgacc taagcatata
    55981 aacaccaagt cctttcgttc cacctagcca ttctgcaaca gttgctgcca ttacagcata
    56041 tgttgcagat attttaagtc cagaaaaaaa tttatccatt gccataggaa atttaaggtg
    56101 aatgaatgta ttaactttat ttgagtccat agtcttaaat aaatttaaat aatctttatc
    56161 aatattctca atgccatcta caaaacttat taatatagga aaaaaacaag taagcattac
    56221 catcaatact tttggcattg ttccaaatcc aaaccatatc ataaagagtg gtgctataat
    56281 tattgtaggt atcatctgtg taaccaacat aataggataa agacattttt ttattattga
    56341 tacaaaatcc atcaatattc caattaccaa tgatattata attgcgacta taaaaccaag
    56401 tattgcttct cctatagtaa ccacagcatg catggataaa ttttgataat ctttaaaaaa
    56461 cacattaatt atatctactg gtgtgggtag tatatactga ggaacttcta aaactcctac
    56521 catagattgc caaagaataa gtataaacaa aaatgttgct attggatata tactatcttt
    56581 gactttatct atgatttcta agtttttcat ctatagtcag acctccagct ttttcatcta
    56641 ctctagctac tgttatgact ctttcacatc caagattaaa agggatttta tgtacctttt
    56701 ttaataattc aaaaacttca tcaaaatcac cttcaacagt agtgctcatt gctcctattt
    56761 cacatttaag accacttttt tcaatttcag caatacaagc atctactact ttatacatct
    56821 cttcttcacc tttgtaaggt cttagtggca ttactgctat gtctgcgatt accatttaaa
    56881 tcactccttt aaaaataatt aatacctact aggtatattt tgttttttat taaaaaattt
    56941 tattactaaa aagatattat atttaaataa aagaaaagtc ttatccaaaa taggataaga
    57001 cttattttta tcttaactgg tctccctaca ctggcattat ccaaacaggt tcaaagggtc
    57061 gagataaaat ctcctctcag cacattagct ccccgaaata tattatatct tttctacttg
    57121 attatactac acttatcata ttttaacaaa ttttttattt atattagata ttttatccgt
    57181 tcaaaaatat ctaatataaa ttgtacatta ttacaccaaa taaattatct taaaatggta
    57241 ttttatcaac atcttccaaa actagattaa ctgaattttc tagtttttat tttgctattt
    57301 caagcatatt tggatgtata tcaattccat acaaatattg tgctaaattt ctgttttcac
    57361 tcatctaatt tgcaatcgtt acagattaca gattagaaac agtttttaga ctctacttat
    57421 ctttatctac aaaaaatctt tcaataaatt cattaataat attcagtaat ctaaaaataa
    57481 acctctatct ggatttttaa gatacaaacc ttttaggaag tatcttttgt gataatataa
    57541 aaattactcg tcattatcat caaaatcctc agcaaccaac attttatata agaatcaaac
    57601 tctataatat aattatttta tccaatttat ttttgagaaa taaaatattt ttttaatttt
    57661 taaatttttt aatatgcacc ttgaaaaaag aaacgttttc ctatattatg gtaatagtag
    57721 aataaaacta aataggcaaa actagagaaa tttagtgacg caaagctata gggactaaga
    57781 cttatgaaat gagttatgtc agccagttgc caaagagata ttttcttttt gtttttatga
    57841 atgtttttta gggggatata tgatatatgt ttatggataa aatggataga tgtacacata
    57901 tactaactgc atatatatgc agttcacgcg attactgcaa ttttatagat acacaattaa
    57961 atgattttat actagaatat ggagagaatg tggtagaatc ttgtttgcat caagtgatgg
    58021 tattggtaag cagatataat taaagaaatt ttattttcta gaaaaccctt tttagtttta
    58081 tctacgtttt ataattcatt aaagaactaa ttaaaataag cttcattata tatttttcat
    58141 gaagtaaaca gttaagttta tctcatatac tgtttacccc aattacataa ttctaataat
    58201 atagattcca atgatttacc acgtttagta gacaaatatt caacttttgg aggaatagtc
    58261 ggataagaaa tcctcttaat aagtccatct ctttcaagat actttaattc ttgtgacagt
    58321 atcttatgac ttatatgaac aacttctttt tttaattgac tgtaacgtat tggctcttgt
    58381 tttaaaatat gccataaaat aattgccctc catttacttc ctatcacagt catggtaaaa
    58441 ccaattgaac aattaaaatc tttttgtctt tcttcaatat ttggaaattc ttcaaaatta
    58501 ttatctatat ccatctcaat cctctctttc aaaatagtaa gtatctaact aaaaagtgca
    58561 ttcttgttta gcgtactaat tcgtaatata atcttgatta aaaaatagct atgcacactc
    58621 gtgactttag tcatgggagg ttcagtgtag catagagcct tacataatac tactttttta
    58681 gatgtatatg tttaaatttt aatatagaca tatgttattt tattaatttt atttaaacag
    58741 ataataataa ataataattt atctcaagtt ttctatgtag ataaactaat ctaatgttta
    58801 gaaaggatta tataatatga ataaaaaatt tcgtctttat caagttgact cattacaaaa
    58861 gaaaaattta caggtaatcc agctggtgta attacaaatg ctgatggctt atctgaatca
    58921 caaatgcaaa aaatagcaag agaattaaat aattcagaaa ctgcttttat tttcaataac
    58981 ttggctaaaa atcatcaaga aaattatgat gtacatatca gattttttac accaactaat
    59041 gaggttccta tatgtggtca tgctactatt gctgcccatt atgttagagc aatagaaaac
    59101 caattcaata acaaaacagt atttcaaaaa actggtgctg gaatattacc agttgatatt
    59161 ataaaaaaag ataatgatta tgaaataatt atgacacaag gaaaaattga agtatctcca
    59221 ccattacaac aaacctatca aaaaagatta cttaatgcat ttggtattta aaggaagata
    59281 ttcgtacaga ttgtcctatt gccatatcat ctacaggaca ttcaaaagtt atgattggaa
    59341 taaaggaatt atcaacatta aatagtttat ctccaaattt atatgaatta tcgaaaataa
    59401 gtcaagaaat aaaatgtaat ggatattata tttttacatt acataataaa gaaactctac
    59461 aagttcatgg aagaatgttt gctcctgcaa ttggtatttc agaagaccct gttacaggaa
    59521 atgctaatgg acccttaggt gcgtaccttg tacattataa agtaatacct acacccaaca
    59581 cagagtttat atttgaagca gttcaaggag aagcaataaa acgcaaaggc aagatgttag
    59641 taaaagtaat tattttagat aataagccag ttgaagtaaa aataattgga aaagctgtaa
    59701 ttgcattctc aacaaacata gaaatctaat tatagattcc ttctaagtaa atttttgaac
    59761 tttttaatac taatttaata acaaactaat ttatatacaa tcaaattaat ttttttatta
    59821 tttgttaata tatatttatc attatattat ataataatat agatagttac tatatcttat
    59881 aattagataa atcataatta tatattattg ttaaaactca attatataat ttttaataat
    59941 attattttag gaggtaaaaa tgataaacga tattaaaaat aaattcaaaa attacaaacc
    60001 atatataaat ggatacaaga atatgaagag agcatctgtt ttaattccaa tagtagaaat
    60061 aaataacaca cactatatat tatttgaagt tcgctcaaaa aatttgaagc atcaacctag
    60121 cgaaatttct tttccaggtg gtaaaataga atcaggtgag actccatatg aagctgttat
    60181 tagagaaact tgtgaggaac ttggaacttt tagtgacaat atagaagtaa tatcaagtct
    60241 tgacctcctt ataacaccag taaagtttat catacatcca tacttaggat atataaaaaa
    60301 tattaataat ttaaatataa acaaggatga agttgaccat acttttttgg ttcctataaa
    60361 atatctttta gagaatcctc caaatactta tagtaatact gtaaaagttt ttccagatga
    60421 aaatctacca tatggttcaa ttccaaacca aaaagattat aaatttgaaa gtggaagtta
    60481 tcctattctg ttttataaat ataaagatta tgtaatttgg ggaatgacag caaaaatact
    60541 agaaaacttt ttaaattttt tatcagaata acactcataa taaattatca aaatttttac
    60601 ataagaaatt tataaaagtt tctaaattta acaattctaa aatatcaata actatatttt
    60661 gtagcacttt tttattttat tattgtttga atatttgatg gttttcaatg caatttcaac
    60721 ataaatacta tatattagtg tatactgttg atgaattaaa gtatgataat ctatttaata
    60781 aagtatgaag taaatgattt taatgtgcct ttatggtaaa attactaata agtaataaaa
    60841 ttaattaaaa tatatggagg tccttataat gtttgctgta ggatttgttg ttataattgc
    60901 acttgccttt ttcattttaa gtgtactatt atttaatgga aaagccagta tgctactagc
    60961 aggttataac tctatgagta agaaagaaaa agcacgatac gataaaaaaa aattatgtag
    61021 aagttctagc attgttactt ttattgtctc tataatgcta tttattatgg cgtttttggg
    61081 atatagaata gagagtggtc aaatggacga aaaaaaaatg gttccctttt ctataatatt
    61141 tataatagtc atactttcca gtgtattttt taatatcctc tactctaaca aaaaatgtaa
    61201 aaaataaata gttgtaagtt gagcagttaa ttataaaaga ttgactgctt tttcatgtat
    61261 attgactact cttattaaat attttaacat ttaaaaatct atgtaaaaat aatgaatata
    61321 gtacaaactt ctaataatga ttttaccttt gtggttacag tccactatat ataatttaat
    61381 atgataacag ggaattatta atgacaacta agtaaacttt tataaaatat aataaaacaa
    61441 aaatagatgt acaatgaaat tatatcttat atcaaattta tttttattaa tagcaaaaac
    61501 ttacattagc ataacatatg acaaattcgc ttaaaataaa aatttttaat ataatttgtt
    61561 ctatagtaca tctattttga ttagtttaca taattttatt attttgtaca atattaatta
    61621 tttactaata tcccatttaa tgaaaaagtt ctaggttcag taattttgac cataactaat
    61681 ttaccaatta aatcatcatt tccaccttcg aaatttacga gtttattttg tctagttcta
    61741 ccagcaaact tattttcatt atttttactt cttccttcaa ctagaacttc aactattcta
    61801 tctttatatc catcatttat ttctgcagaa atcctattta cagcttctaa caccctatta
    61861 aatctatcat gctttatatc ttctggtatt tgattttcca ttttagcagc aggagttccc
    61921 tgtcttttag aataaataaa tgtaaaagct gaatcatatc taactttttc aaccacatct
    61981 aaagtgtcca ataaatcttc ttctgtttct cctgggaaac caatcattaa gtctgtagaa
    62041 aaagcaatac ctggaacttc ctttttagct ttttctatta tttctaaata atattccttt
    62101 gtataatgtc tattcatttt tttcaaaagg ctactgcttc cgcattgtat tggtagatgt
    62161 aaaaactcac acaccttatc acaatctcta attgcgtata ttacttcatc agatatatct
    62221 tttggatgag atgtcataaa tcgaaccctt tcaattcctt ctatatcatt cacttttctt
    62281 aaaagttctg agaatgaaac tggattttca agtgtcttac cataagaatc tacattttgt
    62341 ccaagtaaag tgatttcttt cgttccattt gcaacaagct cttttatctc gttaattata
    62401 tcttcaggag ttctacttct ttctcttcct cttgtgtatg ggactataca atatgtgcag
    62461 aaattattgc atccatacat tatatttaca aatgctttta attcaaattt tctattactt
    62521 ctaagacctt ctattacttc tccatccaca tcccatacat ccactaacat tttgtcagaa
    62581 tttattgatt ctgtaagtaa ttgcggaaat ttatatagat tatgtgttcc aaatataagg
    62641 tctacatgtt tatatttttt tcttaattcc tctacaacat gtggttgttg catcatacat
    62701 ccacatacag ctattttcat atctggattt tttcctttta gatgttttaa ttgtcctaag
    62761 ttaccatata cttttagctc tgcattttct ctaactgcac aggtgttata tattattaaa
    62821 tcactttctt caaccatcat tgacatttgg tatcccatct cttctaacat tgaacataat
    62881 ttttctgaat catgttcatt catttgacac ccaaaggttt gagtaaacac gagttttctc
    62941 tttccagttc tgttgaagta ttcttcattt tgtctgtgta tttgttctat atatttatct
    63001 tgttcttgta ttttctctat aggtattgtt acttgttttc ttttgctcac gtctattcct
    63061 cctagttaat ttcttatatt acacataacc ttaattatat caaataagaa aaatctttta
    63121 aagatattat tatttttcta gttgtgtttt aatagtaaat ctaatgattt atatatacta
    63181 tatttctaaa atataaaatc atataattat tataataata aaaagatgtt ttaaattaaa
    63241 acttaaaaca cctttcctta aataaaatat atgatttact attaaataca attacatcta
    63301 ttcattttca attaaataac aatcatttaa tattaaatat tgaccttcct caaccgttat
    63361 atatcttctt cctgaaaaag tatcattagt aactattccc tctgcattac ctaagctatc
    63421 atttctaact tcataatatg catcagaaga atttgacatt ataatatatt ctccagcagg
    63481 aatttctaat ccaattttat acataccatt tccaaactta ccattcttgg ccttctgggc
    63541 tttagcatac tttgctaatg tcattgttga atcctcaatt ttaatatatt gaccattctt
    63601 taaggtgata tatctagtcc cactaaaaat atcatttgaa agtatactat cagcattacc
    63661 tgtactatca cttgtaactt cataataaga agcataactt cctgagtttg aagttattaa
    63721 gtattcacct gctgagatat cttttccaac cttatacatt ccatctttta tagtctttgt
    63781 agtaggttta acatttttta caaccccagt attaatatct ccaattccat ttgttatatt
    63841 taaacactgt tgtactatat ttgaatctat atatcctata gaagttatct tcttagcatt
    63901 ttttaaaatg cttttattgc taccatcatt aactaaaacc attggaccat gttttgataa
    63961 agaagatcct acaagtgcat ttgttaattc ataagctcca gctatataaa actctctagc
    64021 atccttataa aacttattta ttattgcttt attagtttca aaacgagtac tcccccctag
    64081 tctagtagat ttagtattgt ttactaaagt ggtactcatt gaagctgttc ctcctattac
    64141 ataagacttt aaaccactag ttgaaaaagg tatactttgt ccattagtaa gaattattgg
    64201 tgctttatct cttgctgcta cagatgcaat acttatagca tctgcttctc ctttatatgc
    64261 attggttagc ataacagtat tgactttttt tattgaattt atttcttttg caacattata
    64321 acttgttttt attctgtcat ttcccttaat tcttacaact ttcattttct tagattttaa
    64381 tgaattttcg acagatgtac taatagaata tgttcctcca attatataaa cttttgatac
    64441 attttttaat cttgcagatg tctcagttgg tatagtattt ttttcagtta atagtatagg
    64501 ggcatttaaa gctcctgcta gtccacttgc acttaatcca tctgcaatag agttactagt
    64561 attaattaat atagcagttt tatatgcatt tttatctgct attttacctg cgatttcata
    64621 cttatttttt ccttgtattt tttctacaga tgatattgca tctactaatt gaatatttgc
    64681 aaatacaaaa gaaatactta aagtcaatgt taatattctt ttatagaatt tcataataaa
    64741 attgtccccc tcataataaa tttttaatat ataaatacat attcttaaat tagttatgta
    64801 cttaacaaac ttatgtaaaa ttgcaaatta tattaagcat attacaaata aatattaata
    64861 caattattca tttagctata attgtattta catttatatt aaaaatcaac tagatttgta
    64921 taaatactta taattatatt tttattctta atattttttt tattatgtta ttttaataga
    64981 tttatttttc ttttttaagc atagagatgt atttttccat atattctatt tctattatag
    65041 tttcgcttat ttaattcacc aaaaatttca cttaatatta gaattttaaa tcttttacta
    65101 aaacagactt ttatagttct attaaaattt tttactaaac tttatttttt attatgaatt
    65161 atctattttt tttggctaaa tttaagcaag tttttcacaa aaaatatata cattactata
    65221 tttttaaaac tttataaata tataattgat tta
//
LOCUS       DAYAOY010000009        64320 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYAOY010000009 DAYAOY010000000
VERSION     DAYAOY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.009"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(359..871)
                     /locus_tag="ACGI6B_000642"
     CDS             complement(359..871)
                     /locus_tag="ACGI6B_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9750108.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="ACGI6B_000643"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="ACGI6B_000643"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFG9750109.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            1803..2501
                     /gene="purC"
                     /locus_tag="ACGI6B_000644"
     CDS             1803..2501
                     /gene="purC"
                     /locus_tag="ACGI6B_000644"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFG9750110.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="ACGI6B_000645"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="ACGI6B_000645"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFG9750111.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /gene="purM"
                     /locus_tag="ACGI6B_000646"
     CDS             3874..4950
                     /gene="purM"
                     /locus_tag="ACGI6B_000646"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFG9750112.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /gene="purN"
                     /locus_tag="ACGI6B_000647"
     CDS             4944..5537
                     /gene="purN"
                     /locus_tag="ACGI6B_000647"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFG9750113.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="ACGI6B_000648"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="ACGI6B_000648"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFG9750114.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="ACGI6B_000649"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="ACGI6B_000649"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFG9750115.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="ACGI6B_000650"
     CDS             8348..12154
                     /locus_tag="ACGI6B_000650"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFG9750116.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVAIVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI6B_000651"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI6B_000651"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFG9750117.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI6B_000652"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI6B_000652"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFG9750118.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI6B_000653"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI6B_000653"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFG9750119.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI6B_000654"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI6B_000654"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFG9750120.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="ACGI6B_000655"
     CDS             15867..16535
                     /locus_tag="ACGI6B_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFG9750121.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="ACGI6B_000656"
     CDS             16544..16972
                     /locus_tag="ACGI6B_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HFG9750122.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="ACGI6B_000657"
     CDS             16996..17310
                     /locus_tag="ACGI6B_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9750123.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI6B_000658"
     CDS             17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI6B_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFG9750124.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17741..18157
                     /locus_tag="ACGI6B_000659"
     CDS             17741..18157
                     /locus_tag="ACGI6B_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HFG9750125.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI6B_000660"
     CDS             18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI6B_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFG9750126.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI6B_000661"
     CDS             19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI6B_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFG9750127.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI6B_000662"
     CDS             20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI6B_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HFG9750128.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI6B_000663"
     CDS             20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI6B_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFG9750129.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI6B_000664"
     CDS             21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI6B_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFG9750130.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI6B_000665"
     CDS             21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI6B_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFG9750131.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI6B_000666"
     CDS             21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI6B_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFG9750132.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23370..23705
                     /locus_tag="ACGI6B_000667"
     CDS             23370..23705
                     /locus_tag="ACGI6B_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFG9750133.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23807..24679
                     /locus_tag="ACGI6B_000668"
     CDS             23807..24679
                     /locus_tag="ACGI6B_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFG9750134.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24772..26913
                     /locus_tag="ACGI6B_000669"
     CDS             24772..26913
                     /locus_tag="ACGI6B_000669"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9750135.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26937..28814
                     /locus_tag="ACGI6B_000670"
     CDS             26937..28814
                     /locus_tag="ACGI6B_000670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9750136.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28807..30921
                     /locus_tag="ACGI6B_000671"
     CDS             28807..30921
                     /locus_tag="ACGI6B_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9750137.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30930..31925
                     /locus_tag="ACGI6B_000672"
     CDS             30930..31925
                     /locus_tag="ACGI6B_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HFG9750138.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32017..33249
                     /locus_tag="ACGI6B_000673"
     CDS             32017..33249
                     /locus_tag="ACGI6B_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFG9750139.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33269..34213
                     /locus_tag="ACGI6B_000674"
     CDS             33269..34213
                     /locus_tag="ACGI6B_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HFG9750140.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34286..34714
                     /locus_tag="ACGI6B_000675"
     CDS             34286..34714
                     /locus_tag="ACGI6B_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFG9750141.1"
                     /translation="MNRIDKFMDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVY
                     YIYDVPNNVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGV
                     WHEMFDFDEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34792..35892
                     /locus_tag="ACGI6B_000676"
     CDS             34792..35892
                     /locus_tag="ACGI6B_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFG9750142.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      36201..36291
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            36700..37017
                     /gene="flgB"
                     /locus_tag="ACGI6B_000677"
     CDS             36700..37017
                     /gene="flgB"
                     /locus_tag="ACGI6B_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFG9750143.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            37025..37429
                     /gene="flgC"
                     /locus_tag="ACGI6B_000678"
     CDS             37025..37429
                     /gene="flgC"
                     /locus_tag="ACGI6B_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFG9750144.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            37439..37756
                     /gene="fliE"
                     /locus_tag="ACGI6B_000679"
     CDS             37439..37756
                     /gene="fliE"
                     /locus_tag="ACGI6B_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFG9750145.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            37781..39331
                     /gene="fliF"
                     /locus_tag="ACGI6B_000680"
     CDS             37781..39331
                     /gene="fliF"
                     /locus_tag="ACGI6B_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFG9750146.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            39344..40414
                     /gene="fliG"
                     /locus_tag="ACGI6B_000681"
     CDS             39344..40414
                     /gene="fliG"
                     /locus_tag="ACGI6B_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFG9750147.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            40398..41138
                     /locus_tag="ACGI6B_000682"
     CDS             40398..41138
                     /locus_tag="ACGI6B_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HFG9750148.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            41142..42461
                     /gene="fliI"
                     /locus_tag="ACGI6B_000683"
     CDS             41142..42461
                     /gene="fliI"
                     /locus_tag="ACGI6B_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFG9750149.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            42493..42939
                     /gene="fliJ"
                     /locus_tag="ACGI6B_000684"
     CDS             42493..42939
                     /gene="fliJ"
                     /locus_tag="ACGI6B_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFG9750150.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            42967..44172
                     /locus_tag="ACGI6B_000685"
     CDS             42967..44172
                     /locus_tag="ACGI6B_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFG9750151.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            44190..44798
                     /locus_tag="ACGI6B_000686"
     CDS             44190..44798
                     /locus_tag="ACGI6B_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFG9750152.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            44825..45808
                     /locus_tag="ACGI6B_000687"
     CDS             44825..45808
                     /locus_tag="ACGI6B_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HFG9750153.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            45821..46024
                     /locus_tag="ACGI6B_000688"
     CDS             45821..46024
                     /locus_tag="ACGI6B_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HFG9750154.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            46024..46842
                     /locus_tag="ACGI6B_000689"
     CDS             46024..46842
                     /locus_tag="ACGI6B_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HFG9750155.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            46832..47527
                     /locus_tag="ACGI6B_000690"
     CDS             46832..47527
                     /locus_tag="ACGI6B_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFG9750156.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            47539..47982
                     /gene="fliL"
                     /locus_tag="ACGI6B_000691"
     CDS             47539..47982
                     /gene="fliL"
                     /locus_tag="ACGI6B_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFG9750157.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            47997..48371
                     /locus_tag="ACGI6B_000692"
     CDS             47997..48371
                     /locus_tag="ACGI6B_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HFG9750158.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            48372..49037
                     /gene="fliP"
                     /locus_tag="ACGI6B_000693"
     CDS             48372..49037
                     /gene="fliP"
                     /locus_tag="ACGI6B_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFG9750159.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            49050..49319
                     /gene="fliQ"
                     /locus_tag="ACGI6B_000694"
     CDS             49050..49319
                     /gene="fliQ"
                     /locus_tag="ACGI6B_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFG9750160.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            49331..51145
                     /locus_tag="ACGI6B_000695"
     CDS             49331..51145
                     /locus_tag="ACGI6B_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HFG9750161.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            51154..53229
                     /gene="flhA"
                     /locus_tag="ACGI6B_000696"
     CDS             51154..53229
                     /gene="flhA"
                     /locus_tag="ACGI6B_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFG9750162.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            53204..54220
                     /gene="flhF"
                     /locus_tag="ACGI6B_000697"
     CDS             53204..54220
                     /gene="flhF"
                     /locus_tag="ACGI6B_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HFG9750163.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            54217..55095
                     /locus_tag="ACGI6B_000698"
     CDS             54217..55095
                     /locus_tag="ACGI6B_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HFG9750164.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            55109..55810
                     /locus_tag="ACGI6B_000699"
     CDS             55109..55810
                     /locus_tag="ACGI6B_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9750165.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            55826..56248
                     /locus_tag="ACGI6B_000700"
     CDS             55826..56248
                     /locus_tag="ACGI6B_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFG9750166.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            56233..56418
                     /locus_tag="ACGI6B_000701"
     CDS             56233..56418
                     /locus_tag="ACGI6B_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFG9750167.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            56459..57232
                     /locus_tag="ACGI6B_000702"
     CDS             56459..57232
                     /locus_tag="ACGI6B_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFG9750168.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            57253..58014
                     /locus_tag="ACGI6B_000703"
     CDS             57253..58014
                     /locus_tag="ACGI6B_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFG9750169.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            58037..58918
                     /locus_tag="ACGI6B_000704"
     CDS             58037..58918
                     /locus_tag="ACGI6B_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9750170.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            58921..59295
                     /locus_tag="ACGI6B_000705"
     CDS             58921..59295
                     /locus_tag="ACGI6B_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9750171.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            59327..60730
                     /locus_tag="ACGI6B_000706"
     CDS             59327..60730
                     /locus_tag="ACGI6B_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9750172.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            60846..62783
                     /gene="htpG"
                     /locus_tag="ACGI6B_000707"
     CDS             60846..62783
                     /gene="htpG"
                     /locus_tag="ACGI6B_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFG9750173.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            63171..64301
                     /locus_tag="ACGI6B_000708"
     CDS             63171..64301
                     /locus_tag="ACGI6B_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9750174.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT        26477 a         6606 c        11779 g        19458 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca actggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta gtcaaagcta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt aatggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaacgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac gtagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca ataatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaatgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaggatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taattgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggtgcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat agaaaaaata gttggtggaa ttgcagatgg ttgcaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtggga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacgatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctgtagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag gattaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgattat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtgggggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aatgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attacggagc taaagtaact ggtgctacag
     5341 ttcattttgt tgatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacata aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag ataatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtat gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tagttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtagtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 aaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaaa aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttagga ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actggacaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaacg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tatagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtagag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga aactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttagtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaaactag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgctgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggaggaca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataaa gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgtttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtaaatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttaggg gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aaggtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaagttatc tgtaagatta
    17281 tctaaaatag aagattcaga tgtaaattag cattttaaat atatcccttt agtctattct
    17341 gtatgagttt agctaagggg atatttttat aaaaaattta aaatatgtgt gaattatata
    17401 aatattttta ttttatgagc gataactaaa ttataagtat gtaattttgg aggtgaaact
    17461 atggatatta aaaatgtaag ttcaaatatt gtagtgaata attatttaaa gaatacatct
    17521 ggtataaaaa aagtggaaga ggttaaaaaa attaataaaa cagacaagat agagttatct
    17581 aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg atgagaatat tagaaataaa
    17641 aaagtcgatg aaataaaaaa tgcattagaa aatggaactt acaaaataaa accaaaagaa
    17701 attgcgaaaa aaatgataga ggatatgcga ggataaagtc atgattacag acttaaaagt
    17761 tataatatat gaagaaaaaa atataattag taagatgatg gaattattaa aagaacaatt
    17821 tgattatata gttgaaaaag acttagaaaa tttaaatatg ttaaatccta aactagaaga
    17881 tattagcagg gagcttgcat ccatagaaat aaagagaaga caactttttg gtaatgatat
    17941 ttcaatgagt gaagttgttg aaaatagtaa tgatggatat ttaaaagaaa tatatacaga
    18001 cttgaaacgc attcttgctt tagctaaaaa tcaacaagaa tcaaatgatt ctctaataaa
    18061 gaaagaactt atttttacga aaaaaatgat taatttcata aaaccagctg ataagcaaac
    18121 tacaacttat aattcttatg gaaatattag aaaataaata ttaggaggag aaatatggca
    18181 ggattatttt ccattttaaa tacagctaat tcaggaatga atgcgcacca aaaagcaata
    18241 cagacaattt ctcataatat aagtaatcta gatacagacg gttattctag gcaaaggacc
    18301 gagtttgcta caaatagtcc aatgtatatg cctagtttga gtaattcaat tgcaagagga
    18361 caattaggga ctggagtaca tgtaacggat gtaacaagag ctagaaatag tttttatgat
    18421 taccaattta gggcagaagc tcataagtat ggtaagacag tttcaaaata tgattattac
    18481 aatactatag aaactatact aaacgaacca tcagactatg gaatatctgc aggtatagat
    18541 gattttttta aaggatggga gtcattatca aaggatataa atagtgacag taaaaaaagt
    18601 ctagtggtag aaaacgcatc taatttagca actttaataa gtgaaagtta taagaagctt
    18661 gataagttaa aagaagatat agattctaat atagacactg agataaaaca aataaatgac
    18721 atgttaaaaa gtcttgaaga tttaaataaa tctattgata ttatatctgg ttctggctct
    18781 acaccaaatg atttgcttga tgaaagagat agaattttag ataacttgag ttttaaacta
    18841 gactttgaaa atagtgatgt taaaaatatg cttgcagatg gtaaattaga acttaatgaa
    18901 ttaaaaaatc cagatggaac ttggaaaaca ggtatatcag ggaccttaca aggcttgttt
    18961 gaaatgcatg gtaagattga tacatataaa agcgatttaa aagatgtatc tgatggatta
    19021 gctaaacaga taaatgatgt ttataatacg tctgcaggaa taacagtaac agattttttt
    19081 ataacttcaa atgtagatgg tgaagatatt ataaagataa atccagctat aaagagtaac
    19141 ccaaatgaat taaagcttac tgctgatgaa gcttcaaaaa ttgctaagtt aaaggatgaa
    19201 aaaatagata ttggggtggc tggtggaaaa gtaagtacaa taagtgacca ctataaagcc
    19261 tttgcagaat caatcggttt agattctcaa aaagtaaatc aagatgaagc aaatcaacgt
    19321 aaaattataa ataatattga taactcaaga atgagtgttt caggtgtatc tttggatgaa
    19381 gaaatgacag agcttataaa aatacaacgt tcatatcaag ctagtgcaaa agttatgtca
    19441 acagctgtgc aactactgga tgttgtaata aatggaataa tttaatagaa gggggaattt
    19501 ataatgagag tcagtacagg tatgatgtct agcagctatc taaactcact acaagataat
    19561 ctacaaagat tggataaggt taatagacaa gttaatacaa caaaagaaat aaacaagcta
    19621 tcagataatc catataaagc aataaagata ttaaactcaa aaagtgaaat aaagactatg
    19681 gagacttatg ttgagaattg taaagataca gggaattggt tagaaactac tgatactgca
    19741 ttagatcagc ttggaaaatt attatcagac ataaagaaaa ctttggtggc aagtggtgat
    19801 ggtgcatact cagatgatga attaaagact cttagtaatg aaactaatga aaaaatgaaa
    19861 gaaattgcaa atgctttaaa tgcaacacat gaagggaagt acatattttc aggttcaaat
    19921 acgggggtac cacctgttga atgtatagat aatgctgatg gttcagtgag tttaaagttt
    19981 aattctagtc taaatcttaa taaattaaat gactctttga gtatggatat agctcaagga
    20041 ataagtgtag attataacgt taaattgagt agccttggat ttgacccaac aaaaactgac
    20101 ccatttgaag agttaaataa catatcaaaa aaattagcaa atccagatgc tgatgctata
    20161 aaagagctta caacaacttg tttgggcgat atggaacaac taatagaaaa tacagtaaat
    20221 gttaggtcta tatatgggac aaaagcaaat acagtagagg ctatgaagga aaaaaacgat
    20281 gatagtttga tacagttaaa ggatattttg tcacaaaatg aagaaataga ttatggtgaa
    20341 aaacttgttc aattaaaggc agcagagtta acttatcaag cttcactaca aacaggagga
    20401 aagttattta atgtatctat attagattac atataataaa ataacgcaga tatgatgaag
    20461 gttacattaa aaaaaggtat attaggtttt gaaaatttaa aagaatatga gcttttagat
    20521 atagaagata atgattattt aaaagaattt aattctactg aagaagaagg tataggattt
    20581 gttgttgtat ctccatttga gattatcaat gaatatgaaa tagttttaaa tcaggaaaca
    20641 atagaaaaac tagaagtgaa atctccaaag gatgttgtat tatttaatat aattactctt
    20701 ggtcagacat ttgaagaatc aactgtgaac atgaaagctc ctatagtgat aaatataaaa
    20761 actaattttg gaatgcaaat aattttacag gatgataaat attcaataag gcatccatta
    20821 ttgagaggtg ataatggatg ctagtaattt caagaaaaaa agatgaagca gtacttatag
    20881 gagaaagcat agaagtaaaa attgtaggag tagatggaaa caatgtaaaa ctagctatat
    20941 cagcgcctaa caatataagt atattaagaa aagaaatata cgaaaaagta aagagtgaga
    21001 atataaaagc tacaaataaa aatataaaaa ttttaaagtc attaaaataa taagcttgga
    21061 ggaagtaata tatgtatgga gaaaatcctt ataattctta taaacaaaat gctgttttta
    21121 tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg agcagtaaaa tataccaaaa
    21181 tagccagagc agctatctta gataaaaata cacagaaagc gcatagagaa cttgtaagag
    21241 ttcaggatat atttacagaa ctaatggtta cattagacca aaatgcagga caatgggcaa
    21301 aggatatgta caaagtttat gattttgtaa gatatgaact ttcaagagcc aatattagaa
    21361 aagacatcca ggtaatagat aatgtgttac ctgtaataga acaaattaaa gatacatggc
    21421 atgaagctga taaaaaaagt aaagaagaaa gaagtaggta caaataatgg aattaaattt
    21481 agctgaatta gataagttat caaaagagga attactattg atgcttgtag atggcacagt
    21541 aaaatatacc aatatatcca aagaagctct tttaaataac gactatttga aagcacataa
    21601 tgaacttgta agagttcaaa atatatttac agaactaatg actacattag accaaaatgc
    21661 aggacaatgg gcaaaggata tgtataaagt ttatgaattt ataaaatctg aattagcaat
    21721 agctgatgaa aataaagata ttaaaataat tgatgatata ttgccaatag taaaacaaat
    21781 tagagataca tggcatgaag tttacaatca actaaaaata taattagatg gagggataaa
    21841 ggtatgtcaa gtataagtcc tataagagtt acaggtcttt caggaaattt tgatatggaa
    21901 ggcataatcg aagctagtat gattagagac aaggaaaaag ttgataaagc aaaacaagaa
    21961 caacaaatcg ttaaatggaa gcaagaaata tatagaaatg ttatacaaga atcaaaagat
    22021 ctttatgata aatatctaag cataaattct cctaatagta tagtaagtga aaaagcatac
    22081 tcttctacaa gaataaccag ttctgatgaa agtattatag tagcaaaagg ttcagctggt
    22141 gcagaaaaaa taaattatca atttgcagtt tctcaaatgg ctgaaccagc aaaatttact
    22201 attaaattaa attcaagtga acctattgtt cgacagttcc ctccaaatgc cagtggagcg
    22261 agttctttaa ctataggaga tgtaaatata ccaatatctg aacaagatac tacaagtact
    22321 attgtaagta agataaactc cctttgcgca gataatgata taaaggcttc ttatagtgag
    22381 atgacaggtg aattgattat ttcaagaaaa cagactggtt cgtcatcaga cattaattta
    22441 agagtaattg gaaatgacaa tttagctcag caaattgcta atgataatgg tatcacattt
    22501 ataaatgatg ctggtggaaa caaagtggca aatgtatatg gaaagaatct agaagctgat
    22561 gtaactgatg aacatggaag agtaactcat ataagtaaag aacaaaattc atttaatata
    22621 gataatattg actataatgt aaattcaaaa ggaactgcaa agttgacttc tgtcactgat
    22681 actgaagaag ctgttaaaaa tatgcaagca tttgtggatg attataataa actgatggac
    22741 aaggtctatg gtttagttac tactaaaaaa ccaaaagatt atccgcctct tacagatgcc
    22801 caaaaagaag atatgacaac tgaagaaata gaaaaatggg aaaagaaagc taaagaaggt
    22861 atacttagaa atgatgatga gttaagaggt tttgttgaag atattcaatc tgcatttttt
    22921 ggagatggaa aaaatattat tgcattaaga aaactaggta tcaatgaaag tgaaaattac
    22981 aataaaaaag gtcaaatatc atttaatgca gatacttttt caaaggctct tatagatgat
    23041 agtgataagg tatacaaaac actagcaggt tattcttcga attatgatga taagggaatg
    23101 tttgaaaagc taaaagatat tgtatatgaa tattctggaa gttcaacttc taaacttcct
    23161 aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa atgtatattc aaaacaaatt
    23221 gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa tgaatgataa agagaaaaga
    23281 ctttatgcta aatattcagc cttagaatct ttgttgaatc agtattcttc ccaaatgaat
    23341 tatttctcac aagcacaggg taattaatta tggataaatt aaaagaaaaa ttaaatttat
    23401 ataaagatat atctttacaa attataaatt tgattgaaaa ggaagaatat ataaagataa
    23461 gtattaagtt aggtgaaaga caagagatta taaatagtgt aagtgaaatt gatagaaatg
    23521 attttataca gctttataat agaatggaat taattgaaat tgatagcagg ataagagata
    23581 ttttacaagg acaattatta gaagtaaaaa aagaattaca tgagtataag cttactaaac
    23641 aagtgaacac aatgtattat aacttaaata gagaaaaagt aaatattttt aataaaaaag
    23701 tataaagtta tgtaccatct tgtccgatta tataaatgaa gaattaataa aaaagattaa
    23761 aagtagaaat gacaaggatg tcaactatac taaggagggt aaaataatga gagttaatac
    23821 aaatgtaagt gctttgatag caaataacca aatgggaaga aacgtaaatg cacaaagtaa
    23881 gtctatggag aagctatctt ctggtgttag aattaagaga gctgctgacg atgctgctgg
    23941 gcttgctata tctgagaaaa tgagagctca aataaaaggt ttagaccaag caggaagaaa
    24001 cgttcaagat ggtatatctg ttgtacaaac agcagaaggt gcattagaag aaactggtaa
    24061 catactacaa agaatgagaa ctttatcagt acaatcttca aatgaaacta atactgctga
    24121 agaaagacaa aagatagctg atgagttact acaattaaaa gatgaagttg aaagaatatc
    24181 aagctcaatt gagtttaatg gtaagaaatt acttgatgga agttctacag aaataagatt
    24241 acaagttgga gcaaactttg gaacaaatgt tgcaggaaca tctaacaaca acaatgaaat
    24301 aaaagttgct ctagtaaata cttcaagtat aatgagtaaa gcaggtataa caagttctac
    24361 aatagcaagc ttaaatgcag atggaacttc tggaacaaat gcagctaaac aaatggtatc
    24421 tagcttagat gtagctctta aagaattaaa tacatcaaga gcaaaattag gggcacaaca
    24481 aaatagacta gaatcaactc aaaacaactt aaataatact atagaaaatg ttacagcagc
    24541 tgaatcaaga ataagagata cagatgttgc ttcagaaatg gttaacttat ctaaaatgaa
    24601 tatattagtt caagcatcac agtcaatgct tgctcaagct aatcaacaac cacaaggagt
    24661 tttacaatta ttaggataaa ataattttgt atattggata tagaagaaag ggtaaggctt
    24721 tgctttactc ctttcttttt ttacaaatat acttttgaaa ggaaaattta tatgttactt
    24781 agtatagtta tgattgttaa aaatgaagaa aaaatacttg aaaaaacttt aaaatcatta
    24841 accacattaa gaaattctat agaaagtgaa cttataatag tcgatacagg ttctacagat
    24901 gatactataa aaatatctaa gaagtacacg gaaaaagtgt attttcataa ttggaataat
    24961 gatttttcta gtatgagaaa tatatcaata tcctatgcta aaggtgaatg gttacttata
    25021 ttagatgcag atgaaatact aattgattct tcaactattg taaaattttt taaagatggt
    25081 cttgataaaa aatttaattc tgcttcaata caattaaaaa atctttactc atatgataaa
    25141 aaattatatg gatattgttc agtgcttaga ctttttaaaa acgttgattt tagataccat
    25201 ggtaaagtac atgaacaacc aatatacaaa aaccctatat ttaataatgt tgctgatttt
    25261 gaacattatg gatatttgtt tgaagatgaa gaaataagat taaataaggt gaaaagaaat
    25321 gaaaaactat tatttcaaga gttaaaagag aatgataatt ctccatatac aaactatcaa
    25381 ttagggaaaa attttattat attaggaaaa tatcaggagg ctttatacta tcttgagaaa
    25441 tcttatgaat tatattctaa actagaattg gcacctggtt acttgataac aagccttgta
    25501 aaaacatatt tatatttagg aaaacataag aagtgtgaaa gattgtgctt aaagtatatt
    25561 aagaaagaca tgaataatat tgatatatat tattatattg cacaagcaca agtagattta
    25621 ggaaagtatg aaaatagtat agattcgtat aagagatata tttacttgtt agataattat
    25681 gaaataagta cacaagcaaa tagtttattt tcagacactg atactgtagg gcttagagat
    25741 gaggctatta ttacactgat aaagatttat tataagcttg aaaaatatga gtttgtaatt
    25801 tcagagtata ataacattga agatgtggaa aagaaaaaga atgtgtactt tagcttattt
    25861 atgtcactat ataaattgaa tagatttgaa gatataaaaa attattataa agaagtttca
    25921 aattctaaaa ttgaaaaaaa tagtttttat agaaatatag aagaagttat aaataatatt
    25981 aaagaggatg aaaaagagta tgtatataaa atcctttgtg acattgaagg taattatggc
    26041 attttaaatc aaataaggat aaataaatgt gtatctatag ataaatgtaa agaaatactc
    26101 aatgaagaaa aggaaataat ttatgcatat attataaaaa tagcttttga agagaagatt
    26161 gatttactag atatttttta tgaaatggaa tatgtatgga tagagaaata tctaaaatat
    26221 cttttagcat tagaaagaag ttttaattta gtactatata agtacataat tagtcgtcca
    26281 aatacatcag atatggaaaa gataagagta tataaagcta tatctaaagt agttttagaa
    26341 agtataagct taagtgatga aaaatataag gagatttttt atctttatat aatgtattct
    26401 tataagtaca taagatacat atatagcaat ttaagtgatt ttgaattaat aaaatttgtt
    26461 tgtaatgatt tagataagtt cactatagaa tttaagcatc tacttgatat taaaaataaa
    26521 gtaaataatg ctaatgaaaa attagagtat atacatagta tgaggaaatt actaaatgag
    26581 tatccttttt ataataaaat aataaaaata cttataggtg aattagaaga aaatataaaa
    26641 gaaagtgaag aatttaagga tttaaaagat agttttatta ttagtataga aaatatgatt
    26701 gaaactggca aaataaatga tgcgaaatta ttggtagatg actattcaaa aacatttgtg
    26761 gatgatataa agattttaaa tataaaggga attttataca tgtttgaaaa taagcttgat
    26821 aaagctgatt ttatgttaaa aaaggcatta tcattagatg tagaaaatga agatactata
    26881 tataatatag aatacttaaa aagtttaaga taagtcaaaa ataggagaat tgaagaatga
    26941 atataccaat agtggttaat gaaaaatcta aagacataat aaaagattgg caatatacat
    27001 gtacaaatga agatataaaa tttacaagca taataatatt gacatataat caaatagaat
    27061 atactaaatt atgtattgaa agtattagga aatttacccc aaagaataaa tatgaaataa
    27121 taataattga taataactct tctgatggaa cagttgagtg gttaaaggga caatctgata
    27181 taaaatctgt gtataataac aaaaacttag gatttccaaa aggatgcaat caaggtattg
    27241 agttagctac tggtgaaaac atattgttgt taaataatga tgtaatagtt actccaaatt
    27301 ggctctataa tttgaataaa gctttatggt ctcaagataa tataggtgct gtaggaccta
    27361 ttacgaatgc atgttcatat tatcagcaaa ttgatgttga ttataagtca attgaggaca
    27421 tgtttatatt tgcacacaaa ataaataaat ctaatattaa cctatggaaa tatagattga
    27481 agttagttgg attttgcatg ttaataaaaa aaactgttct ggataatgta ggggttttag
    27541 atgaattatt cacacctgga aattttgaag atgatgattt atcttttagg atgatagagt
    27601 caggatataa gttattatta tgccaggata cttttataca ccattttgga agtgtgtctt
    27661 ttaaagaaaa tcgtaataaa tatacagata taatgtatat taatagaaga aaatttgaaa
    27721 ctaagtgggg atttaatcca gaatatagtg gaatgataag aacagatttg atttctaaaa
    27781 tagatgatag tttggaggca aatattaatg ttttagaaat aggatgcggt ataggggcta
    27841 ctcttttaga aataaaaaat atatataaaa atgcatcaat atatggaata gaaattagtg
    27901 agtcagcagg taaacttgca aagtatagtg cagatgtact tatagcaaat atagaaaaag
    27961 taaaattgga ttataaaaaa gatttttttg attatataat attgggagat gtacttgaac
    28021 atttgattga cccatggaag atagtcaatg aattgaaaca atatctaaag aaggatggtt
    28081 atctggtatc tagtatacca aatattatgc atgtttctgt tttaagaaat cttgtgttag
    28141 gtaattttac atatgaagat tcaggaattt tggacaagac tcatttaagg ttttttacat
    28201 taaatgagat aacaaaaatg tttaatgata gtaattacat aattaatagt attacagcta
    28261 ctagtatgcc actttcggaa attgataata gcttcattga tacaatatgt aaggaaagct
    28321 gtaaagaaat gagacaacag tacgaatctt atcaatatat agtaaaagcc tctaaaatcc
    28381 tagatactaa aagatattct agtattagta tgattaattt aaaatacgct ttaatgagaa
    28441 ttgataatga attagatgta gatagtaatt taagttatat atttgataat tataatataa
    28501 ctgaaccatg ttttgttgat gatattgaat atctaattga tacatttgtg ataaataaag
    28561 caactctatt aaatagatta ggagttgagg catttaacag aggtctagga gattttagta
    28621 taaacatgtt tatgacagta ttagagatgg atagagatga tattgactca atatataata
    28681 taacaagtgt attatgtgaa ttagaagaat ttgatactgc ttataattta attaaaaata
    28741 gtagcgaaaa agtacagagt gaaagtgata tacaagagat actttgtttt attgagggga
    28801 agaaatatga ataatgataa gatatgtttt ataacctgtg taaatgatga aaggctttat
    28861 gaagaatgtc ttgtatatat aaatagccta gacattccta aaaactttag tatagaaaca
    28921 gtagctttaa gaggagccga ttctattgcc tctgcttata ataaagctat gagagaaaca
    28981 gattctaagt ataaggtata cttacatcaa gatactttta taattaataa aaatttcata
    29041 catgatattt tagagatatt taaaaaaaat acagatgtcg gaatgattgg tgtagctgga
    29101 gtaaaagata tgcctgtgaa tggaatttgg tgggattctg ataaaaaagc agggaaagta
    29161 tatgatagtc atacaggtaa aatggatatt tatgaattta gtaactttga agaatgtttt
    29221 aatgaagtta tggctataga tggattaatt atgattactc aatatgatgt attttggaga
    29281 gaggataagt ttgatgggtg gcatttttat gatttgtcac agagtatgga gtttagacaa
    29341 aataaataca aagttattgt accaaaacaa aataagcctt ggtgtatgca tgattctgga
    29401 gtagtaaata taaataatgg atatgatgaa tataggataa aatttgtaaa agagtacata
    29461 aatcttgatt tatatagaga aaatttttat gaatttgata agaagaaagc aagtaccata
    29521 cttaagagta ataagcatgt aatggattgt atgaaagagg agcttacaga acaattaaga
    29581 ttaaagaatt acaaggatgt tttaggtatt gtttctaaaa ttgctcattt ttctgttttt
    29641 aatcatactg gtttgtattc ttgtgaagaa tgtgagagag cattgctaga atgtgcaaat
    29701 aatttaccag aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa aagaagaaaa
    29761 gtattacatg ttttaagtga aggatattca acaggaggtc atacaagact agttaaaaat
    29821 tggattgaaa ttgacaaaga ctctgttcat tcagttgtaa ctacatggca atcagataca
    29881 ctgccagact ggttattaaa agcaataaat gatagtggtg gcgaagttat atctttggat
    29941 ttaatttcag atgattattt aaaaagagct gtagctttga ggaaaatagc ttatgactgg
    30001 gctgatatag ttattttata tgttcatatg tgggatacaa tacctgttgt tgcttttgga
    30061 gttgatggga aaatacctat tttacatgtt aatcactatg atagtacttt ttggttaggg
    30121 acaagtattg cagatattgt cattgatttt agcgaagcta gtcaattaat tacattagat
    30181 aaaagaggca tagataaatc tgaaatatta ccaatgccat taaaagaaaa agaagtattt
    30241 tcaaaatctg aagttagaaa acgatataat ttagaggatg atgtgacaat aatattgact
    30301 attgctagtg aaagaaaata caatagtgta aatgatatta attactttaa aattgctaaa
    30361 acattgattg aaaatacaga taatacaatt attattgttg taggagttga tacagagaat
    30421 ctctattgga aaactttaag taacgttaca gataatagag ttatatgttt tggaacaaca
    30481 gcaaatatag acgattttta caagatttcg gatatatata tggagtcttt catggtagga
    30541 agtcatactt caaaactaga tgcaatttta tatgatttaa ttcctgttaa gtttaaaaat
    30601 gaagtttcac caatttttac aaatatgtgg aattttatag ataagtttga ttataagaat
    30661 ataaaagagg ttatagatat gataaaaagg tacaatacta gtgaatttga cagggatagg
    30721 ataaaagaaa aacaatcgga aataaaacat tttgttatag ataaacactg tattgaaata
    30781 aaaaactata tagacaaaat atataatgaa gctaatcatc ataaagttaa gtatgattta
    30841 tataaaaatg atgaaatcga ggactatcag ctttttgtag cattatatgg ctatatggta
    30901 aaaaataaaa acatgtcata ggtggtaaaa tgaacaatat tcatatcaat aaatttgaaa
    30961 aaagctgtga aagaaactgg gattattttg tagaaaataa atctataaat ggtacatttc
    31021 tgcaaactag aaatttcctt aattatcatg aggagggaaa attcactgac cactcattaa
    31081 taatatgtaa aggtgaagaa atactagcag tcgtacctgc atgtgaaact tatgaagatg
    31141 gtgaaagaat atttttttct catttaggga gtacttttgg tggtattata ataagaagtg
    31201 gttttaatga tattaggcat gtaaatgcca ttacaaataa gttagaaata tatttacggg
    31261 aacaaaattt cgataaagta tatttaaaaa atacatcaag aatattttgt aaagaggata
    31321 cttcattact agaatatttt ttatttaaga ataattacat gttttatgat gaattaagta
    31381 tgtttttaga attaagtaag tataaagaag atatattagc caattttaaa tacagtaaaa
    31441 gaagagattt taaatattct ttgaagaata atcttacctt taaaagactg tatagtgatg
    31501 aagatatagt ttgttttttt aatttacttt ctaattgttt agatagtaaa ggagctaaag
    31561 taatacattc agtagaagaa cttttggagt ttaaaaataa tagattaaaa gatattgtag
    31621 aattttatgc agtatatatt gatgacatta tgatttgtgg gagcatggta tttaactttc
    31681 aagaaagagt ttatcatact caatatctag catcaaattc gacatataat aaatattatc
    31741 caatgatatt tttatactat aatctaataa aagatgcttt agagagaaat tttaagtatc
    31801 tttcttttgg aattagcact ctaaatagag gcaaatactt agacgagggt ttagctacat
    31861 ttaaagaagg ttttggttca agtgcaattt gtaacagaac atactataaa aatataaata
    31921 agtgagaatc atgttttata gagtttttag aatatagtaa atatctagta ctctaacatt
    31981 acataaaaat aattagttta taaagaaggt ttgtatatga aaagattaat gattttaggc
    32041 tcaattaagt attttgagaa tattgttata tcagctaaaa aagagggaat atatacgatt
    32101 gtttgtgata atagaattga tactcctgcg aaaaagattt gtgatgaagc aatagatgtt
    32161 gatgtatttg attttgataa aataaaaagt atagctatag ataaaaagat agatggaatt
    32221 ttaactggat ttacagattc tttaatgaaa ccatatgttt atattgcaaa tgaactaaat
    32281 ttaccatgtg tgatttcttg caatcaactt gaatctgtaa ctaacaaggc tattatgaaa
    32341 aaatatttta aaaataatgc aatacctaca actgattact gtattattga aagtctaaaa
    32401 gatttagaaa aaatacaagg attaaatttt ccacttgtaa taaaacctgt tgatggttat
    32461 ggttcaagag gtgtatattt tgtatcaaat atagaagaat taatagaaaa atttgaatat
    32521 tctaaggaag cctcacaaga tggacaagta ataattgagg aattttatga aagtgaggaa
    32581 atacaaggac tagcttgggt tcataatgga gagtcacatg tattttatat tggagataga
    32641 gagcttgtaa acatacatac aggaagagct ggaaaaccag atagattact ttatccatca
    32701 aagtattgtt ttcaatatga ggaggaaata aaatctatat atcaaaatat aacacaggct
    32761 tttgatataa aaaatggacc tctttatgta caaatgttag tgggagcata tggaataaag
    32821 gtatctgaag taatgccaag attacctgga ggttgtgact atatggctat ttctcaaata
    32881 acagatttag acataggaag tttatttgca aactttagtg tgaataatga agtaaatttt
    32941 gatgaaataa agaaatataa tatgcatcta tcaaaatgtg tatatgcatt accaatatac
    33001 ataagacccg ggacaataaa aaagataaat aatatagata aaatagaaaa cttagattat
    33061 gtcactcaat ttctattact agtaaaagaa ggagatacaa tagaattata tgaagatgag
    33121 agacaggatt gtggaagagt ttatggaata gcaaataata tatttgatgc aaatataaaa
    33181 aagaaatgta ttcatgaaat gatagaaata cttgatgaaa ataatgaaaa tatgatagaa
    33241 aatttttaaa taagggagta tgatagatat gaagctaata actcatgaac aaattatgtc
    33301 attaaacatt tctcagaaac aaggatatga gtggataaaa gaatctttct taacaaaaaa
    33361 agattgtcaa ttaccaccaa aaataagcat ggtagaagaa ggacatgtat tttacaatgt
    33421 aatgccatcc ctaaatcata atcatatgat tggtggaata aaaatggtta caagattgcc
    33481 agggagaaat ccaagtttaa actcagagat atttctatac aatatggaag atgctaaatt
    33541 aaaggctatt ttggatggta attatattac agcttttagg actgctgctg ttgctgcctt
    33601 ggctatcgat acatttgcta aaaaagattt taatactatt gctattatgg gtttaggtgt
    33661 aactgcttct gctataatga atatgctttc atatactctt aaagataaaa atataaatat
    33721 aaagttactt aaatataaag accaagcaga gttgtttgta aaaaagtatg aaaaaaatga
    33781 acaatttagt tttgaaatat gtgatacaca tgaagaactt attgtagatt cagatgtagt
    33841 tatttctact gtgacatata cagatgaata tttatcagaa tttaaatggt ttaaaaaagg
    33901 tgctttatta ttacctgtac atgttatggg atttggaaat gttgattatc tatttgataa
    33961 agtatattta gatgattata atcatttgaa acatctagaa aatatagata gatataaata
    34021 ttcttgtgaa ttttatgatg ttttaagtaa acaagctaaa ggaagagaaa atgatgatga
    34081 gagaataata tcttacaata taggtttgtc aatacatgat atattatttg caaataaaat
    34141 ttacgaattg tgtgaaacac taagctgtga agaaatagaa ttaaaagaac caaaagaaaa
    34201 atattggata tagtttattt cattcatatt tacaatagtt tattgtaaat atgaatatga
    34261 agtaatatat tgttagaaaa ttagcatgaa taggatagat aaatttatgg ataaatataa
    34321 tttaattagg ttcgtatcta ttggaaacga atatatagga aatttagttg cattagaatc
    34381 aaataaagta ataccttttg atataaagag agtttattac atatatgatg taccaaacaa
    34441 tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa gtactagttt gcctaaatgg
    34501 aagtgtgaag ataaaatgtt atgatggtgt aaaagagact atatttaatt tacataaaaa
    34561 tgatgttgga ttatatatag gaacgggagt gtggcatgag atgtttgatt ttgatgaaaa
    34621 aactgtttta atttctatag catctgaaca ttataatgaa gaagattata ttagagacta
    34681 taaagaattt ttaaagtatt taaataatgt gtagttatta tacaattagt gatgtatttt
    34741 cttaattaaa taaatttaag atagccacat agagaataga tagattagga tgtgaattta
    34801 ttgttggtta aatttaataa tttattaaaa cagttccaaa ctcataaaga agaatatgaa
    34861 cagaagatta aggatgtaat ggaaagtgga tattatatcc ttggaaatga agtaaagcat
    34921 tttgaagaag aatttgctaa ctatgtaggt agcaaatatt gtgttggttt agccagtggt
    34981 ttagatgcac tgataattgc ttttagagca ttaggaataa gcaaaggtga tgaagtaatc
    35041 gtgcaatcaa atacatatat tgctagtgtt atgggaatca gtataaatgg agcaacacca
    35101 gtatttgtag aaccaaatga atattataat atagattcat ctaagataga agaaaagata
    35161 aatagcaaaa caaaagctat attagtagtg catttatatg gtcaagcaag taatatgaaa
    35221 gatatacaag aattatgtga taagtataac ttgtatttaa ttgaagattg tgctcaatct
    35281 catggagcta aatatgatgg aaaaatgaca ggaacatttg gtgatatagg ttgttttagt
    35341 ttttatccaa gtaaaaattt gggtgcattt ggagatgcag gagctatagt aactagtaat
    35401 gaggaaatag ctgataaagt gaaaattctt agaaactatg gctcagaaaa gcgatattat
    35461 aataaatttg tcggatataa ttcaagattg gacgaaattc aggcaggttt attaagagta
    35521 aaacttaaat atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga tagatatctt
    35581 aatgaaatta ataatcccaa agtcatactt ccaaagatag aaaataattg tactcatgta
    35641 tggcatttat ttgttttaca agttgaaaaa cgagatacat ttatggaata cttaaagaaa
    35701 agtggtatag agtctataat acattatccg ataccaccac atctatctga agcatatgct
    35761 tatttgggat ataaaaaggg gagttttaat atagctgaga actattcaga taaagttgtt
    35821 agtatacctt tatttattgg tatggaagat gaagaagtta gttatataat taaaattata
    35881 aatgaatatt aaactactca taataatatc aaaaataaat tttttatata tcatttattt
    35941 aaatttcgtt aattgacatt tttaaatatt gatatattgt acaaataaaa ttgaaatata
    36001 tggaaaaata tagaattaag atgagatttg tcttatattt ttataaatta aaaatatttt
    36061 caaagagttt attataaaat tgtaaactat agtgtttata atctttgtaa ttattggtaa
    36121 ttaaatttaa aaaataaaat atttaattat acacaaaaaa gatatttaaa aaatttgtta
    36181 tttattgtat acttaagtta aaactaaata ggcaaatcta gagaaatcta gtgacgcaaa
    36241 gctataggga ctaaggttta tatacataaa ctatgtcagc cagttgccaa aaagagtcct
    36301 aggtgtattg tatacctaag aaaagtctat aatgactgcc tttttggcag tcattttgtt
    36361 atggaaaaat cactttattt cagtagaaaa tacacataat aacatatttt accaattata
    36421 acataaaata aagaaaaatt atctcaaaaa gttactattt tacaaaaaag agcaactttt
    36481 cgaagaaata tttaaataca tattaaaatt tttttatatt ttcatgggac ttcattaaaa
    36541 aaatattagt tttcttacta aattgataca ttattgtatt aatgtaatgg aaatgcgatt
    36601 aatttaaata aataaagaag tcattttttg taatatagca acttcatttt ttttattaat
    36661 aaacaaaata gataaaatat attttgagga ggggtgagtg tgaatacata tgacattata
    36721 aaaatgggat tagatgcaac taatctaaga agtcagacaa tagcaaataa tatagcaaat
    36781 ataaatactg aaggatataa aagacattat ataacttttg aagaaacact aaatgatgct
    36841 atgcctaaaa tagaagaaaa aacagacaat tctaaaggtt taagagtaga tggtaataat
    36901 gtagacatag aagaagaaaa agtaaatcaa gcaatggcaa cattacaata taatgctttg
    36961 ataagttttg caaatggaaa aatagcaatg accaaatcaa taataagtgg gaggtaatta
    37021 gaagatgagt gtgttcagtg gaatgagaat atctgctagt ggattaacag cagaaagttt
    37081 gaaaatggat atcatatcat ccaatgtagc taatataaat gctacaagag gtccaaatgg
    37141 agaagcatat aggagaaaag ttgtttcatt tgaagaaaac tatgataata aattaggtat
    37201 gctaggagta aaagttacat ctgttgaaga tgataaatca cctcttaaag aagtatatga
    37261 cccatctcat ccagatgcaa agccaaatgg atatgtagaa tatccaaatg taaatatatt
    37321 ggtagaaatg acagatttaa taacagcttc acgagcatat gattctaacg tagatacatt
    37381 gaatgctcag aagtctatga tatcaaaagc tttggaaata ggaaagtagg gataaattat
    37441 gccaattaca caaattaaca atttaatgca aggtgcaaag ttagataatt tgattggaga
    37501 agcaaagcca ttaactctta ataataaaga agaaaaaaag aattttgaag atgtaataat
    37561 agatactata gataaagtta attttcaaca agtaacagca gatgatatga aagaaaaatt
    37621 tataaaaggc gaagatgttt ctatgcatga agttatgtta ataggtcaag aagcacaact
    37681 atctatgcaa tatttaatag aagttagaaa taagattcat gatgcatatc aagaaatgaa
    37741 taaggtgcaa atttagcctt ttgaacagga gtaataatca atgataacca gttttaaaaa
    37801 taagattaag gagcaagttg ctaaatctag gacaaagata agttctttaa aaaagggaac
    37861 taaaatagca ttaggtatag gggttattgc tatatgttta gcgctagtat ttacagcaat
    37921 atctgcgagc aaaaataagt atgcagtctt attttcagaa ctagatgctc aagatgctgc
    37981 agatatatca gcagagctag agtctaaaaa gatagatact aaggtaaaag gaagtacgat
    38041 ttatgtacct agaacagaag ttgataagtt aaggctggag ttagcgccaa gtcttacagg
    38101 tggttctaaa ggatttgcac taatggatga tagttcatca atagggctta ctgatgaaga
    38161 atttcagatt aagaaacaaa gaatgataca aggtgaaata gaaaaaacaa taaagagttt
    38221 tccacaagtg gaagacacta gagtacatat aacatttgga gagtcatcag tatttgagaa
    38281 ggaggctgtt ccagggagtg cagcagttta tttgatgcta aaaccaggga cgactttaga
    38341 aaaaaaccaa ataaagtctg ttatggcact tatatcagga agcgttacaa atataccaga
    38401 gaaaaatgtt caggttattg ataacaaaat gaatttatta tctgaaggta ttttggagga
    38461 tgaaaatgtt gatgaaaata atcaaccaat aaatagtggc attagtgttg ataaaaataa
    38521 ggcatctgaa caagcattga ataaacaact agaaaagtct attttagaaa tgctagaacc
    38581 gatatttggt aagggaaagg tgaaagctac agtaaatgct gatttaagtt ttgatacagc
    38641 tgaaaaaact gagataaaaa ttgaccctga taaagttgca ataaaggaga caaggtcgaa
    38701 aaattcatct aaatctgctg caaataatgt tcaaggtgta gatgctaaca tgaataacca
    38761 aggaaataac aatggtacta atacagaaga tagtttagat gagagttttg agtatgaaac
    38821 aggtaagatt gaaactcaca ctatagttgc gcctggagaa cttaatagat tgacagcttc
    38881 tgttgcaata gatggaaaaa tagctaaagg tgttcaagct gatgtagagg atattgttaa
    38941 taatgctata ggtatggatg gagcgagggg agatactctt agcgttgttt caatggtatt
    39001 tgacccagaa ggtaagaaag aggctaaaca agagctggaa gagattaaaa aagaaggatt
    39061 taaaaagaac ataataaaag cagttattgg agtagtagca gtaatagcag ttggagctat
    39121 tggatactta atatataaga aaagaagtga acgagaagat atggattatg aagatgaaga
    39181 ttatatttct aaatctgatt ctgttgatag gaaggataat cctctattag gagatgaccc
    39241 agaactgaca ttagaggaag ctgttaaaat gtatgcagaa gaaaagccag accaagtaac
    39301 agagataata aagacttggt taaatgagta agggggatag aaagtgtcca cggaaattac
    39361 aaatgttaat gcgtttgact taagtgatat aaatgaaagt gataagaatg agaaggtaga
    39421 aggtgagggt ttaaaaaaag ctgctgtatt attaatgaca ttaggcccaa atgtgtctag
    39481 tgatataata aaaagcttgc ctgataaaca agttcaaaaa ataggtttgg aaattgcaaa
    39541 tatttcttca atagcttcaa aagaaagaag agaaatctta aaagagtttg tagaaataaa
    39601 tagggcaaaa gattatgtta tagaaggtgg gcttgaatat gcaagagagt tattaagtaa
    39661 tgctttagga aatgttaaag caaacaaatt acttgaaggt atatcaataa atacaagtac
    39721 aaagcctttt aaacatatta taaaactaga tgtaaagaag atattagaaa tacttaggaa
    39781 tgaaagtgca caaactatag ccgttttact tgcacatatg gaaccagaaa atggagcaga
    39841 agttttatca tacttagatt ttgatttaca aaatgaagta gctatgagaa taagctcaat
    39901 gggttcaata tctccagaag taataaaggt aattgaaagt tcattagaag ataaattaca
    39961 ttcaataagt ggtgatgatt ttaatgatta tggaggggtg tctaccttag ttcaaatatt
    40021 gattaattct gatagaaaaa cagaaaagaa tattttggga actattgaag aacaaaaccc
    40081 ggatttagca gctcaaatta aatctcatat atttgtcttt gaagatattg tcaagttaga
    40141 tgatataagc atacaaaaag tgttaaaaga agtgactatg aaagatttag tatatgcctt
    40201 aaaaggtgta gatgatgaga tttcagatgc tatatataga aatcaatctt ctagagcagc
    40261 agatgcactt aaagaagaaa tggaaatggt tggtgttata aaggttacac aagttgaggc
    40321 ttctcaacaa aagatagcta atatcgtaag aaaacttgaa agagaaggtg ccataactat
    40381 tgtaaggaat tttggtggtg actttgttga ataataagat tattaaaagt aaaaaagcat
    40441 tatataaagg agtgctagat ttaaccataa tagataaagt tagagtggga gaatcaaatg
    40501 taaataaaga aaatgattgt agatttaggg atgaggtaat aaaagaagct atagaatctg
    40561 ctgagataag aaagaatgag atattagaag aagcaaaaat gaatgcacaa aatatagaaa
    40621 agcaagcata tgaaaaaggg tatagtcaag gtcaaaaaaa tggatatgaa gatggatata
    40681 aagagtcata tgatgaatat acccaaaaag ctaaagatga agcagatttt ataaaaaatc
    40741 aggcagactt aatactatta caagctaatg agaagatgac tgagtacata aaagaaaaaa
    40801 ataaagagat tgtaagactt gctattaata tggctagttc tgttctaaaa aagcatttcg
    40861 aagatgaaga atctatgagt gatttgttaa agcaaattat tcaagattat gaaggaagta
    40921 gttcatttat agtaagatgt aattctttct ataaagataa tatagagaaa gagtttgaat
    40981 tattcaaaga ggaaacatca ctaaaatcaa gtgtatttgt tattgcagat gatagtatag
    41041 ataaaggcaa tgctattatc caaaaggaaa atggaagaat aatagttggt attgattgtg
    41101 ctatggaaaa actaaaagaa gaattgcttg gtgaataaag tatgtataaa actgatatat
    41161 ctatagattt tgacaaaata aataagaaaa tagaaaattt agaactcact atatcagaag
    41221 gtagagtaaa gaagataata ggacttactg tagaagttga aggaataaaa gcttttgttg
    41281 gggagttatg cgttatatac aaccaagtta ataaaccagt gaattgtgaa gtagtaggtt
    41341 ttaaagataa agaagtcata ttaatggcgt tgggagaatt aacattgata gcacctggtt
    41401 gtaaagttat ttctaagggg atacctttaa gtgttatgtg tagtgataat ttactaggta
    41461 aagtacttga tggtcttgga aatcctatag acaactcaga tgctgtttta ggagatagat
    41521 acaacctgaa caatgaacct cctgacccaa tgaagagaaa aaagatacga aatataatgg
    41581 aaacaggtgt aagagcaata gatgcattta ctacttgtgg tgaaggacag agaatcggca
    41641 tatttgcagg aagtggagta ggtaagagta cgactctagg tatgattgct aggaatgcaa
    41701 aagcagatgt aaatgtaatt gctcttattg gagaaagagg aagagaagta ctagatttta
    41761 ttgataaaga tttaggtgaa gagggaatga aaaaatctgt tgttgtgtgt gcaacatcag
    41821 ataaagcccc tctagttagg ttaaaaggtg ctcttactgc tactgcaata gcagaatatt
    41881 ttagagacca aggcaaaaaa gtaatactta tgatggattc agtaactaga tttgctatgg
    41941 cacaaagaga agttggtgta gcaataggag agccaccagc tcaaaaagga tatacaccat
    42001 ctgtatttgc aatgttgcca aaacttatgg agagaacagg gacatcagat aaaggttcta
    42061 taactgcatt ttatacagta ttagttgatg gagatgactt taatgagcca atagcagata
    42121 caacaagagg gatacttgat ggtcatatag ttttatctag agacttggca aataagaatc
    42181 actatccttc tatagatgta ttaaacagtt taagtaggct tatgaatgaa attgcttcaa
    42241 aagaagacat aaaaatagcg tcttttgcaa gagacatgct tgctgagtat agagaagcag
    42301 aagatttgat aaatataggt gcatatgctt caggtacaaa caaaaaaata gatgaagcaa
    42361 tctattatca tgagcatatt attaattttt taaaacaagg aataaatgaa aagagttctt
    42421 ttaatgaaac tataagtagt ttaagaagaa tctttgaata aacaatttaa aaaaatattt
    42481 gaataggttg tgatggaatt ggataaaaat tttaagttta gattacagaa agttttagac
    42541 ttaaaaataa aagatgaaga agagataaaa atggaatttg ctaagattca acaaaaaaaa
    42601 atagatatag aaactaattt agaaaactta gaaagtaatt acagcaaata ttctataagt
    42661 aaaaataatg ataatgttca aaatcaaaaa ataactatta actatttgct agctttaaac
    42721 aactctataa tggatttatc agaagaactg gataagtcga caaatgaact tgaaaaagct
    42781 agaaaacagc ttataagcaa acagatagag agaaagtcat tagaaaaatt aaaagaaaaa
    42841 aaatatggac agtattataa agaagaacag ttaaaagaac aaagcactaa tgatgagttt
    42901 gcaagcatga gttatttgag aaatagacaa gttctataaa ataataggaa gtgaggatat
    42961 tattgtatga agatacaagc agattcttat aatattatta aagatattgg gaatagaaaa
    43021 aactcatcta agaataaaaa tataaaaaat aataaatttg aagataatct atataagaca
    43081 acagaccata aaaatgttaa agatagaaga gaaacaagta agcaaaatgt tagttcagat
    43141 agaccgggaa atagtgataa tgagtgtcat ttagatgaag ataaaagtaa tcacttagaa
    43201 gatgaaaata catatgaaaa agattgtagt aagaataatg aagctttaca gcaattactg
    43261 aatcttttaa aagagaatta taaaaaagat aaagatataa acactatatt gaataatata
    43321 aaaaaactaa gtatatcaga tgaattaaaa caagaactta agcttaataa aaatatattg
    43381 aataaaattg caaatcaaaa tttagagtta aatggagcta atacaaataa ccctaatgat
    43441 atagaaaatt tagacataat gaagttaaga gaacttttag aaaataatgc tagtgaagat
    43501 attcaagtaa ttagtaaaac tacagaaaat gatactaaaa gcatagactt atacagtttt
    43561 gatagtaata gaatggatac tcttaactca aataaacaga aagatgatag ttctgatatt
    43621 ttggaaaaac tagcagggtt aaatggaaat aagagtggaa attttggaca agtaataaat
    43681 aaaagcgttg atatgaataa aattaaaaat gataatgtta atttaaaaca gatagaatct
    43741 aatgtaatgg atgattctat aaaagctatt aagcatatga aaacaaatga tatacaagaa
    43801 ttgacaatta aattaagacc aaaagaatta ggagatatga acatacaatt attaaaagat
    43861 ggtgaaagta tgagagctgt tgtgactgtt ttcaataaag atgtctttga ctctataaat
    43921 aaaaacatag cagatttaaa gcaacatctt gaacttacaa atgtaaatat aaaagatgta
    43981 tcagtgcaaa tgcatagcga taatacaaat acatctgata cctttgataa agcatttgaa
    44041 caacagaata gacaaaataa ccaagaaaac atgagtaatc aaaatacaaa tgataagcgt
    44101 aacaatgtag tcatagaaga tgagattaaa gaaaatatta ttgatgatga tagagtagat
    44161 ttactagctt agtaaggggg aagtattgca tgtacggaat agcaacggct ttggcaggaa
    44221 ctcaggtaaa ctataggtca aataacttaa gccaacttga aagaaatatt aatgatattg
    44281 ccaacaaaca agttagaagt aaaggaaatc agacagtaac agacaatggg actccaatta
    44341 taggacaagg tgatgatgct gataaagact tgtttatgaa gattcttgta gcacagatgg
    44401 gtaatcaaga cccaatgaat ccacaagacc caacggagta tgtatcacaa ttagcacagt
    44461 ttgcaacttt agaaaaaatg tcagctatga atgataagct agatatactt ttagccttaa
    44521 gtaactctac tctaataaac tctgctttga gtatggcaac atcattaatt ggtaaagaag
    44581 tagaagtatc tggggaagaa gatgaagatg aaaacattgt aggagtagta aagtccacat
    44641 acataaaaga tggggaagta cacttagaat tagaagtaga tggaagtgac aaaactgtag
    44701 atgttaaata tgaaaaatta cttaaaatag gagaggtaga ggcaaattca ggagaagaaa
    44761 atgactcttc agaacaagaa tctccagaag aagtataata ttaaatggga aaagaggtaa
    44821 atttatgata aaagcaatgt attcaggtgt aagtggaatg aaagcaaatc aaacaaaact
    44881 agatgtaatc ggtaacaatg tagccaacgc tggaactact tcttttaaga aaagttcagc
    44941 tagaataaca gattcatttt atcaaactgt actatatgca agtgctccaa cagcggcatt
    45001 agggggaaca aacttaggac aagtaggtgt aggtagtaag atatcaagta taaacaaaga
    45061 tatgacacaa ggaaatgtac aaccaactaa tagaccatct gatttaatga tagatggaga
    45121 tggatatctt ccagtagtaa gacaaggaac agttatgtat acgagagatg gttcttttaa
    45181 tttagataca ggatttaaag cagctgatgg tgctttgaag aatattaaag gtggaagact
    45241 tgtaaatggt gatggatata tgttacaagg tatgatttat gatggggaat ataaagctga
    45301 tactggagaa tttgttaaaa agggtgatgc taaaggaaaa gagcctattg taataccact
    45361 tcaaagtata agtcctaagg atggaacagt tcaaaatgta ctagagtata atatatcaaa
    45421 ggatggaaca gtagaatttt tattgagtga tggtcaaaga actcaatttg tctatgatat
    45481 acctgatggt gctaaggtag gagatacacc tgctggaata gataaattaa aaggtacaat
    45541 acaaaaagtg caaacatatg cattccaaaa tccagcagga ctagatgcag aaggaggaaa
    45601 cttgttcaag ccatcagcaa actcaggaga cccacaaata gcaggagcta caggcacaat
    45661 aatacaagga gctatagaag cttccaatgt agatatagca gaagaattta cagaaatgat
    45721 aatagcaagt aggtctttcc aagcaaacag taaaataata tcgacatcag acgaaatact
    45781 tcaagaaata ataaatctta agagatagta ggtacatatt atgataagag taacggattt
    45841 aaacggaaga aaatacatat taaattcaga tcttatagtg agaattgact ccgtgccaga
    45901 aagcaggatt ctccttacaa caggagaaaa aaatcttgta aaagagtcag tagatgagat
    45961 agtagataaa gtaataaagt ataaaagaaa aataaatgta ggttatatta ggagtgaaaa
    46021 ataatgaaaa aaacagattc actaacattc attggactaa tattatctac agtattagtt
    46081 ttatttggag cagtaaaagg aagtagttca ggattgaaaa gttttttcga ttttgcatct
    46141 atattaatca cagtatttgg gtcatttgga gctttgatga ttacatttac tattgacgat
    46201 ataaaactta taaaagatgc tctacaatac tcattcaaga caatgaatat atcgaaatta
    46261 gacttattaa atcaatttaa aacactttct aaaaaagcaa gaagagaagg acttttatct
    46321 atagaaagtg atgtaatgaa aatacaagac ccatttatga aaaaaggatt agagttatgt
    46381 attgatggtc ttgaaataaa tgaaataagg tctatactag agctcgaaat agattctgtt
    46441 gaagaaacca ataacagata ttctaaacta tttaaaactt gggggacttt cgcacctgca
    46501 tttggtatgt tgggaactct aataggttta atacaaatgt tatccgacct tacatctcca
    46561 gatttaatag catctggtat gggaaaagca ttaattacga cattttatgg gtctttactt
    46621 gccaatatcg ctttgaaccc aattgcatat aatatagatg agaagactga aaaagaaatt
    46681 tatgtaaagg aaatgatgct ggaggggata ataagcatac aatctggaga gagcagcata
    46741 gtagttgagg aacgtttagc tacatacctt tcaaataatg agaagttaga aattatgaaa
    46801 tccaataaaa atacagaaag agcaatgagt aatggcgcgt aaaaaaaata agaaaaagga
    46861 tgagataaat ccagatgcat ggttggctac ttatgcagat acgataacac tgattcttac
    46921 attttttgtg ttattgtatg ctacatcagt tgtagatatg gaaaaattta aaaatatagc
    46981 agatgcgtta aaaggtcagt tttctgggac aagtatgttt agtacaggtt caagtacaag
    47041 tgagaaatct ccaatagaga gtgttttagt ggataaaaat gataagtcaa atttagaaca
    47101 ggatttaaaa gataaagtta ataattctaa cttacaaaat agtgtaacag tcaaagatga
    47161 tagtagaggg attttactag agctagatga ttcaatttta tttgactctg ggatttctga
    47221 attaaaagaa gatagtaaag ccactcttaa taaagtatac gatatgataa agatgatgaa
    47281 aaataagata gttatagaag gccatactga taatgtacct gtaaaatcaa gtacgcatga
    47341 atccaattgg gatttatctt ctagtagagc agtaagtgtt gtaagatact ttgtagaaga
    47401 aaagggcatg gaccctaaac tattttcggc aacaggatgt ggagagtacc aacctttggt
    47461 atcaaatgaa acagcaaagg gaagagctga gaatagaaga gttaatatac ttataactat
    47521 tgactaggag gaagaggttt gttcaaaaat aaaaaaatta ttataattat tttgatactg
    47581 attattttct cagcaggagc aggttttttt atattaggag aaggtggaaa taaacaaatt
    47641 gacaagattg caggtttgtt tattccaaca gggaaaggca aagatgaagt aaagaccgaa
    47701 aatcttgact tagaagagat ggtattgaag ctagctgatg aaggtgtaag atatttaaaa
    47761 gtatcaataa ctgtatctta tgattcttca tataaagata tagaaaaaaa tacgccttta
    47821 attagggata atattataaa ttgttttatg gcaaaaaggg cagatgattt tactgctgat
    47881 aatttaaaga acataaaaga agaaattaaa aatacattaa atatacaact aggtgaaaat
    47941 gtaattcaaa atatatattt cacaaatata gtagtgcaat aaaaggatgg taagacatga
    48001 tactttattt actaaaaata ttaatatgta tcccaataat tttaatattg atagtcttat
    48061 ctttaaaaat tagccaagga actttattaa aaaaatataa tgataaatat ataaaaatac
    48121 tagatattgt aaatataagt aaaaacaata gcataataat tttaaaaata ggtgaagaag
    48181 gatgcattat ggcatcttct tcttctagca tgagtaaaat aaaagatttg accaaagaag
    48241 aaatattaag aatagaaaac gacattaaag aaagaaattt taaaattcaa gattttaaaa
    48301 tggaaagaat taaaaagaaa tatgatttca gtagatatat tggaaaattt acaaaaaagg
    48361 aagataagta aatggaacta ttatcaaaat tacctttggc tgatgtacca tatacaagtt
    48421 caatgcaatt gtttttattt ctaactgcat taatgttttt accatttgta atgttcatga
    48481 tgacaagctt tgtcagaata gttataagtt tatcattttt gaaatcagca ttaggagcac
    48541 agcaagcaat tccaagtcaa attttggtag ggcttgcaat agtattgaca atatttataa
    48601 tgagaccagt attaaatgag ataaacgaaa aggcattaca accatatatg aaagaagaag
    48661 ttactatgga agaagctatg aaggaagcag aaggacctat taaagaattt ttattaaagc
    48721 aaaccagaca gacagattta gatttatttg tagagcaagc tggtttaaaa gaaaagaaat
    48781 taactcgtga aaatatacca ttatctgttg tagttccagc atttgctata agtgaactaa
    48841 aaactgcttt tcaaataggt tttttaatat atataccatt tttgataata gaccttgttg
    48901 tggcaagtgt tttgatgtct atgggtatgt ttatgttacc tccagttatg atatcattac
    48961 catttaaatt attattattt gtaatggtgg atggatggaa cttgatagta aaaacattga
    49021 tattgggatt tggataggag aaaaatagta tgagtgaatt tatgataatg agtgttctta
    49081 aaaatggcat gtttacagga gctatgattg cagctccagt gctaattgtt tctcttgtag
    49141 tggggctttt aataagtgta tttcaagcca caactcaaat ccaagagcaa actttgactt
    49201 ttgtaccaaa actaatagca attcttttag tagtagctat tggtggaagc tggatgttac
    49261 atactcttat tagctttact tcggagatat tcaatatgat gtctctcata actaaataag
    49321 taggatatat atgatttttg tatttattag aatagtagca ttctttggaa cattaaggat
    49381 actatttcca gctggtacac ctgctttatt taagtcgctc tttgcaatca ttatatcaat
    49441 cttgatttca agtactatgc aaattgaata tgcaacaaat atagacaaca ttgtattgtt
    49501 taccttatat ggtataaatg agaccataac tgggattatg cttggatata taaccaatct
    49561 atgtttttat agtatcagaa tggctggttc tatgatggac caacagatgg gattatcaat
    49621 gattaatatg tttgacccaa attcaatgac tcagacaact ttaatcaata atcttatgaa
    49681 ctggacagca cttatgatat tttttagcat ggatggtcat catgttttaa ttagaggcat
    49741 aaggtatagt tttgagttaa ttccaatagg gaaatcattc atagataata atatagatta
    49801 tataataaac atatttgtgc agtgcttttt aactggattc aaaatagcaa tacctattgt
    49861 actttgtctg cttatggcag attttatatt agggcttata tcaagaagtg tacctcaact
    49921 aaatgtaatg ataattggta tgccattaaa aatactggtt ggtattgcat tatttatcat
    49981 atcaatacct ttaattgtca atcaaataag tcatttatta agtcaaatac caaagatgta
    50041 tgagggaact tttgcactag ctcctatgtt ttttatgggt tcaacagata aaacagaaga
    50101 agctactcca aagaaaaagg gagaacaaag aaaaaaaggt aatatagcaa agagtaaaga
    50161 attacccatt gcaatgactc tacttgcatt cactttatta gtacctacat tattttcata
    50221 tgtagtgaat acacttaaat cttctcttaa ttattttttg agtctagatt tttatatgaa
    50281 cataaattat tcaagtatag agaaattagt tatagcagga ttaatggatt tttttaagat
    50341 atttttacca atagctattc cattcttagt tttaggtata gtcgcaaacc tatttcaagt
    50401 aggaatactg tttactggtg aaactttaaa acccaattta tcaaaattaa atccaattag
    50461 tggattcaaa aacatgtttt ctatgagaag tttgtcaacc ttaataaaag atacagcgat
    50521 tatatctatt ttggcttaca taggatatac attcttccaa gataattatt tagatatatt
    50581 aaagttaggt aacatatacc tgccaacttt aatgtacaca gttaaagatt tagtatattc
    50641 aatattaagt aagatttgtg tagctatgat agttatagca gttgcagatt atgtttatca
    50701 aagatacagt cataaaaagc aacttagaat gacaaaacaa gaagttaaag atgaatataa
    50761 aaactcagaa ggagacccag aaataaaagc taagataaaa caaaaacaaa gacaaatttc
    50821 gtctcaaaga accatgcaag cagtaccaag tgctactgta atagtaacca atcccaccca
    50881 cttatctata gcagtgagat atgaaaaagg gaaagaccaa gctccagtag tggtggcaaa
    50941 aggtgcagat tatttagctt ttaaaataag ggaaattgca aagggaaatg acatacctat
    51001 aatagaaaat aaacctattg ctaggttgtt atataagcaa gtagaaatag accaagaaat
    51061 tccagaggac atgtatcaag cttttgcaga gatattagta gcagtatata aaatcaaaaa
    51121 tagatacaaa gtccccaaaa ggtaggataa gtaatgaata agtttaattt aaaggaaaat
    51181 atggacgtaa tagtggcatt tggtgttgta gggattgttc ttatgatgat agtgccgtta
    51241 cctacattta tattagatat tttactggca attaacataa gtttatctgt gctaatttta
    51301 ctaatgacac tatttacaac aaatgtactt gatttatcta tatttccaac agtgttactt
    51361 gtgacaacac tatttagatt gggattaaat ctttcatcaa ctagattgat tttaacacag
    51421 ggatatgcag gtgaagttat agaggcattt ggaagttttg ttgtaggtgg aaattacgta
    51481 gtaggaataa ttatattctt aattatatta attgttcaat ttgttgttat aaccaatggt
    51541 tctggaagag tatctgaagt aacagctaga tttacgttag atgccatgcc aggtaagcag
    51601 atgagtatag atgcagattt aaatgcagga gttatagatg acaaaactgc tagaaaaaga
    51661 agaaaagagc ttcagcaaga agcagatttt tatggctcta tggatggagc aaataagttt
    51721 gtaaagggag atgccatagc aggtcttata gtaacagcaa taaacataat aggtggaatt
    51781 gtaataggag ctgtaatgct gaatatgact cttatgggtg cagcacagac atatgtaaga
    51841 cttactatag gggatggtct tgtaagccag atacctgctc tattaatatc tacatcagca
    51901 ggtataatcg taacaagagc ttctactgat gaaaactttg gtggattagt aggtaaacag
    51961 ttaacagcaa taccaaaagc agtaatgatg actggctgtg ttctaatcgt acttggtatg
    52021 atgcctggat tacctaaacc agcatttttt tcactaggta taggtgctat agcagcaggt
    52081 tactttttag gaagagaaaa gaaagatact ttagaagact tggagtttga aaaacctgat
    52141 atggtaaatc cagataaaaa tgaagtagaa gatgtaacaa acttaataaa tgtagaatca
    52201 atagaagtag aaattggata tggtttaata tctttagctg atgaatcagc aggaggagat
    52261 ttacttcaga gaatagcatc aataagaaga caatgtgcaa tagacatggg tatagtcgta
    52321 caacctataa gaataagaga taatttacag ttaaatccaa accaatacaa tataaaaata
    52381 aaagggaatg tagttggtgg gtatgagata atgcctaata tggttttatg tatggatcct
    52441 atgaatcaag gatttagtat atttggaata aagacaattg agcctacttt tggaatagag
    52501 gcactttgga tagaaagtga caaggtagaa gatgcagagc taaagggtta tacagttgta
    52561 gattcatcca caatacttat aactcactta ctagatataa taaaatcaaa tgcacacgaa
    52621 ttactgggaa gacaagaagt taagactatt atagatgcag caagagaaaa ttatagtgct
    52681 gtagtagatg aattgatacc agatttaatg acacttggtg aaattcaaaa agtacttaaa
    52741 aatctgttaa gagaaaaagt aaatataaag gatagagtta caatacttga gacacttgca
    52801 gaccagtcta gaaatacaaa agatatagag cttcttactg aatatgtaag aatagctttg
    52861 gctagaagta tttgtaataa tttagttgat gaagataagg caattgtagt ggctacattg
    52921 tctccagaaa ctgaagaatt ggtatctaat aatttacaaa ggtctgtaaa tggaacttat
    52981 ccagcagttg acccagaaaa tacaaataaa atatttgaga gtatacaaga agtaatgaac
    53041 aatgtatatt tcaatcataa tataccagta atagtagtat caccaaagat aagagctcca
    53101 tttagaaaat tagttgaaat tgtatatcct aatttgactg tattatcatt aaatgaaata
    53161 cctaacgata ttaaaatcaa agcagaaggt gtggtgagca tatatgatga cgaaaaagta
    53221 tacagctaac acaattcaag atgctatgaa tctagccaaa atggaattgg gtgacaatat
    53281 tacattaata gataaaaaag aggtaagaaa atctggaata agaggcattt tttcaaagaa
    53341 agatattgaa ttgactattg gatgggaaaa gaaagataat ttagaacaaa aagatttaaa
    53401 acgagagata gaacaactaa agtcaattat aaataatatg gggtttgata ataaaaatga
    53461 taatgatata gataaaatat gcaagaattt actaaactta gatttaaatc aagaaattgt
    53521 agaatttata aaagctgatt tacaagaaat gaagttcaat ggaattgata caagtaaaaa
    53581 tttagtagaa agtctaaaga aaaagattaa aatagaaaat caagcaataa atggtaaaat
    53641 tgctttagtt ggaccaccag gagtaggtaa aacgactaca atagcaaagt tggctgccaa
    53701 attagttttt gaggaaaata aaaaagtagg agtaataact atagatacat acaggattgg
    53761 tgctgtagaa cagttaaaga tatatactga tataatgaat attcctttta aaggggtaat
    53821 aagccctgat gagatggaat tggcacttga tgagatgaaa gattgtgatg ttgtattaat
    53881 agatacaaca ggtagaggct ataaaaactc tatgcaaata ttagaaatca aaaatcttat
    53941 agataaagct gaaactgata atatacattt agttttgaac tgtacaacaa gagaaagtga
    54001 tacaaaagca attatagatt catatagaaa tgtgaatttt aaaagcttaa taattacaaa
    54061 gttagatgaa acaataacat atggctcaat atttaacata atgaattatg cacaaaaacc
    54121 aatatcatat attacaacag gacaaaatgt tcctgatgat attataaaac ctaatgaaga
    54181 taagattata agattactat taggagttga aagtatatga tagaccaagc tcaaattttg
    54241 agaaaaatgg caatggaaaa aagaggttct gatgagatta tagtagagaa tgagaatatg
    54301 cctaagataa ttacaatagc ttctggtaaa ggcggcgttg gaaagagtaa tctcgctaca
    54361 aatttgtcta tatgcttaac caaacttgat aaaaaagttc ttatattaga tgcagatata
    54421 ggaatgtcta atatagatat aataatgggg gtaaatgtaa aagggactat aattgatgta
    54481 attaatggcg aaaaaaatat agaagatata atttcacaga ctaagtatgg tgtaaatata
    54541 atatctggag gttctgccct taatcatata gaagacttta cagaagctca aagaaataag
    54601 tttatacata gcatagaaca gattcacaat gtggatttta taataattga tacaggagct
    54661 ggtatgagca aaagcctatt gtcatttata tactgtagta ctgaattttt cttaataaca
    54721 actccagaac cgacatcatt gactgacgca tatagtttat taaaagctat aagtaatttt
    54781 ggcataaaga aaagtgcaaa tataataatc aatagggtaa ttgatttaga agaagcgaaa
    54841 tctacataca aaagaataaa aatggttgtg gacaaatttc taaaccttaa tctggaattg
    54901 tatggatata taatagatga caaaaaagta ggtgtatgtg taaaaaaaca gataccattc
    54961 attttagaat atcctacaag tattgcatca aaatgtatta tagctatagc aaagagaatg
    55021 gtaagtcagt caagagaaga tattgttgat aataaaagtt ttctaagaaa gatgtttagt
    55081 atatttaaaa gctaaggagg cgtagttaat gaatagagaa gaattaataa aagagaatat
    55141 gcctcttgta aagagtatag caagaaaatt ttttatacca gggaaaggtt ttgaatatca
    55201 agacttagta aatagtggag tcattggatt gattgatgca ataaataaat ttgatactga
    55261 aaaaggagca aagttttcta gttactctta tataaagata aaatctgcta tattagatga
    55321 aattagaaat caaagtccaa tttctaaaca taatctaact aaagtaaata agtataatag
    55381 agtggtagaa aagttgcaat ctaaattatt aagagagcca acttcatatg aaattgcaaa
    55441 agaattaaag gtttcagaaa aagaacttca tgatatcgaa agtaatatag atatgttaaa
    55501 tattgtatca ttaaactatg taatctttga agatacaaat gaaactgtac aagatgtaat
    55561 aagtgataga gaagaggaag ctccagaaaa tataatagaa gaagaagaaa agttagagat
    55621 tctatccaaa gcaattagta atttaaatga aagagaaaaa ttaatacttt cactgtacta
    55681 ttatgaagat ttaaatctaa aggagatagg gaaagtgcta ggtgtttcag aatcaagagt
    55741 ttcacaactc cacagaaaat caataagaaa cttaagaaac aagataaaag aacttaaata
    55801 ttctatatag atggtgattg ataagatgat acaaattata ttaatagtag tgtctatttt
    55861 aataatagta actatagtta taaatttaca gaaggaatca aaaaataaag ataacagata
    55921 ctttgataaa atatttgagc aagaatatga agcaaatgat agattaataa ttgcagaaaa
    55981 aagaagattc tttttagaca aagtacttgc agaaaaaaga attaatagtc atataaaagt
    56041 ttatgaaaat tataacctaa taaaggctaa tgaagaaaga aaggaaaaca tatttataga
    56101 atcaaaaaag gaagtaagac aagaaaatga gttatctctt aaagtgaaac atcttataag
    56161 ttcaggttat gatgatgtgg aaatatgtaa gatgctagat attggaaagg gggaactgtt
    56221 attaataagg agttgttaca aaatataagg atattattat gtgataaaaa agtattctta
    56281 ggaataggta ttggtatatt aatatcaact ttatgtataa tgctttttag taataatgat
    56341 atgagtaagg cagagataga aaaaaaggcc agagcgttag gcatgaaata tacaagtgag
    56401 atgaaagttt tagaatagat gatactgttt agacatttac aaaaaaggag gtcataatgt
    56461 gataagagga ttatatacag cagtttctgc tatgataact aatcaacaaa agcaaaatgt
    56521 tgttgtcaat aacctagcta atatggatac aaatggatat aaaagtaaac aacttttgac
    56581 taaaagtttt gatgaattaa agttggaggg ttacaataac tacgcaaatg gaactaaaca
    56641 aaagcagatt ataggcgata taagtccagg tatttctatg gatgaaacta tcacaaattt
    56701 taatcaagga cctataaaaa ctactgataa taaaatggat gtagctattc aaggtaaggg
    56761 attttttcta gttagtgatg cagctggaaa tcaattttac actagaaatg ggaacttcag
    56821 aacagacaat caaggtgacc ttataacaag tgaagggtat tatgtacttg ggacgaatac
    56881 tgctactggt gctgtaggtc atataaatgt aggaaatcaa aaatttgaag tggcaaaaga
    56941 taatacaata tcactagacg gaaatgctat gtataaattt aatattgtag attttaataa
    57001 ctacaataac ttaaaaaaag aaggagataa cttatattct ggaggaggag ctatgaaagt
    57061 aaataatgca cttacaatac aaagtgcgat agagtcctct aatgtagata tggtatctga
    57121 aattaataat atgatgacaa tatcaagaga atatgaggca aatcaaaaga taattcaggc
    57181 tatggattct aaattagcaa aaatagcaag tgagattggt tcagtaagat aggtaatagg
    57241 aggtgaattt ttatgtacac aatgttttat actagtaaaa cagctttatc agcaaatcaa
    57301 agtaaactag atataatatc taacaatata gcaaattctg attcaacagg atataaaaaa
    57361 ataaatatgg aatattctga tttagtggat gaagtactaa atagaccatc ataccctaca
    57421 aatggtaaag atatatcgac tggaacagga gctaaggcaa cagctcccat gagaaattat
    57481 gaacaaggtg cattggctcc tacagatagt aagagtaatt tggcgataga tggagaagga
    57541 ttctttagag tgataagaaa agatggtaca tatgcatata caagaaatgg tggatttaat
    57601 gtagatgctt taggtaagat tgtagatgat aatggaaata tattagatgt ccaatttgat
    57661 gcaggatata actataataa tacaaatata gatagtacaa acctgacagt ttcaagaaat
    57721 ggagagctat tcgtagataa taaaaaaata ggaaaaatta acttatatca acctatagga
    57781 actcaaaatt ttatatctga aggagatagt ttatttgtcg caactgatgc agcacagatt
    57841 aaacaagttg agaaagtcga tatagttcaa ggctatagag agaggtcaaa tgtttcttta
    57901 caagaagaat ttgttgactt aatagccact caaagagcat ttcaaatgaa ctcaaaggga
    57961 ataaagacag cagatgaaat gtggcaaata gcgaataatt taagagctaa ataattatgt
    58021 aaggagagga atattcatga atgatatgaa agagtacgat tttggcaaac ctcagagtta
    58081 ttcaagagaa aatttaactt gtttaaattt tttattagct gaattttgta aaaaatataa
    58141 aaactatata atttatgaac ttaagtgtaa ttctagtatg gttgttgata gtatagacca
    58201 agtaaattat cagacttttt tagatagagt aacttcttca tgcgtagtag tacaaaatgc
    58261 aatggaacct gttatgaaaa acttttcttt tagaatagat agaagtgtag ctgatatgtg
    58321 gattgatata acttctggag gaactggagt tgtaaaggat aatgatagag agcttacaga
    58381 acttgataaa aaagtgcttt tacaccttat ggatgattta gtaagaaata tgcacttatt
    58441 tgagggtttt gaaaatatac aagtattgga tacacataca catattaatt tgccacaatt
    58501 atgttctcca actgcaccag tatgcgttgt agatttaaaa gtgctaaatg ataatgagtg
    58561 tattggaagt gtttctcttt gttttccata caatggttta gaggctctgt tagaaagtat
    58621 atctgttata gaattttttg ataatgatgt aggagatgat tcagaggaat ttacccaaaa
    58681 aatctataat agtgtatcta atatagaatt atcagttatt gcagagatag gaaaagtatc
    58741 aattaatgtt gaggatttac ttaaactaga agttggagat gtaatagtaa caaataaaaa
    58801 aataaatgat tatatagata tatttgtaga aaattcaaaa tcatacacag caacaccagg
    58861 atttatatca agtaaaaaag gtgtaaaaat aaatgatgct gttggaaaag aggtgtagtt
    58921 atggacaata gtaatcttat ttcagatgaa gaagcaaaga tgttattaaa tgataaagtt
    58981 gaaagtgaag ttgatggaga tgatgagtta gccttagatg tctctgaagt taatcgaaat
    59041 aatatacaaa ggatacttga ccttaagtta gaattatctg tagttatagg aagaactaag
    59101 atgactctaa gagatatttt aaatttacat aaaggttctc ttattgaact ggatacttta
    59161 gcagaccaag atgtggaaat actaatagat aataaggttt tggcttatgg aaaagtagtg
    59221 gtggtagatt taaattttgg tgtaaagata acaagtatag taagtgaaga agacatggta
    59281 aaatctcttg tttagagata acatcaatta atacttaagg catgaggtgg tacttatttt
    59341 aaacttaaat gatataaaaa aagatatact aaaactcaat aaaaaagact catgttctct
    59401 atataatgaa tctattaata atataaataa taattgtata tcaactggat taaaattttt
    59461 aaaacaagca agagatttaa acccagatga tgttgatata ttaaatctca caggtcttgt
    59521 aaacttacta aaatgtaatt ttgatgatgc tgtagaaagt ttctataaga gttcactttg
    59581 tgaaaaaact gctctgtgtg aaaagtatat aaacatgctt acatctgagg agtttttaat
    59641 tttctttgaa aagtacaatc aacttataag gttaataaat aaaggaatgt ataaagaagc
    59701 tatacaaggg tttaaactca taactgacca atactgtgat ttaatagaac cttatgagtt
    59761 gcttgcctta ctgtatgaca aggaagaaga atatataaaa tttgatgaat gtttagaagt
    59821 attgaagatt attgataagg aaaattattt attaaatcaa gaaaataaca cattagatat
    59881 gtataatagt gctaatatac aaactaaaag taatataaaa gtagatgatt ataagaatga
    59941 gttcttacat aaaagtagta gtaaccataa aattaatgat tataatgatg taactaaaga
    60001 aaagagttca agcaaaaacc agaaaagttc agattctaat aagaaaaaaa gtattaaaaa
    60061 aaataagtac ctatatatag ccataatatt aggaatatta ttagtaggac aaagtatata
    60121 taattcttat aagatgaata atttaactga taaaatatct aaaaataatg aaactaaaaa
    60181 tgaagagtca atagattcta acttgactaa gaaagaaaat aatatagaag aaaatgaaga
    60241 tatgagtaag gcttctaata taaataagag taataaaaac attaataaag aggatgatgt
    60301 aaaatctaaa aaaaatactg tagaaaatga agatgataaa gattctgact tatttacaga
    60361 ggatgaattg atgtctaagg cgaatacttt aaaatcagaa aagaaaacta aatcttctat
    60421 acaactatat aagaaagttg cagatatagg tgaaaataaa gggaatacat ctgaagcaac
    60481 ttatcaagtt gctattctca gtgaaaagtt aaaagactat aaaactgctg aagaatatta
    60541 taaaatgtat gtagaaaatt actcagaaaa agatgcttat tttgatgaat cttattataa
    60601 tttaggtatg atgtactata acaatggtga tttaaaaaat tctaaattga cattaaagaa
    60661 gttggttaat aaggtaccaa atagtatgta taataattca aaagtaaaag aaattttaaa
    60721 agaagaatag ttaaattaga taagaaaaat aaattatttt attttaaaat aatatgatta
    60781 taatatattg acataccatt ttaaaaaatt taaaatatta attataaaat gaggagggat
    60841 aaaaaatgga atttgaaaaa gggagtattt caattcatac agaaaatatt tttcctataa
    60901 ttaaaaaatg gttatactct gataaagaca tatttataag agagcttata agtaatggat
    60961 gtgatgcagt aagtaaacat aaaagacttg tatcactagg agaaatatca gaaaataagt
    61021 catcagatta taaaattaca gtatctgtaa ataaaggaga aggtacactt aaatttattg
    61081 acaatggaat tggtatgact gaagaagaga taaaaaaata cataaatcaa gttgcttttt
    61141 caggtgctga agatttcttt aataaatata aggataaaat ggaagagtca aatgatataa
    61201 taggtcattt tggattagga ttttattctg catttatggt gtctaaaaag gtacaaatag
    61261 atacactttc atatacagaa ggagcgactc ctgtaagatg gatatctgaa ggtggtacag
    61321 aatacgaaat atctgaatca gatgctagaa atgatagagg aactactata acactattta
    61381 ttgatgatga tagtaaagaa tttttagatg aatttactgt aagaggaata ataaataaat
    61441 actgttcttt cttaccagta gaaatatatt tagaagatgt tgaaagatta gaaagagaag
    61501 ctaaagaagc agaagaaaaa gctaaaaaac aaaaggaaga tggaaaagaa gagattgtag
    61561 atgcaaaagt aattgagcct ttaaacgaca ctaatcctct atggttaaaa tctccaaaag
    61621 actgtactga tgaggagtat aaagagtttt atagaaaggt attcaatgta tttgatgaac
    61681 ctttattttg gatacattta aatgtagatt atcctttcaa tttaaagggt atactttact
    61741 tccctaaatt aaaaaatgag tttgaactga ctgaaggaaa agtaaaatta tacaacaatc
    61801 aagtgtttgt ggctgacaat ataaaagaag ttataccaga atttttactt ttattaaaag
    61861 gagttataga ctgcccagac ttaccattaa atgtatcaag aagtttctta caaaatgaca
    61921 gggatgtaag caaaatttct aaacatataa ttaaaaaagt agctgataag ttaaaatctc
    61981 tttgtaaaaa tgaaagagaa gaatacaata agttttggga tgatatacaa atatttatta
    62041 aatatggttg cttaaaagat gaaagtttct atgagaaggt aaaagaatgt atattattca
    62101 aaactattga tgatgagtat ataacattac aagattattt agaaaagtgt aaggacaaac
    62161 atgaaaataa ggtattttat gtaagtgata aagaacagca atctcaatac attaaactat
    62221 ttaaagaata cgacttaagt gctgttgttt taaactcatc aatagatact cattttattt
    62281 catttatgga atataaagag aatggagtta agtttaatag aatagatgca gacctttctg
    62341 atgtattaaa agataaaaat gaaaataaag atagtgaaga aaacaaagaa gagatagcta
    62401 agatagaagg tctgtttaaa gaagctgttg gagaaagagt gaagaattat tctgtagaag
    62461 gattgaaaaa tgaagacact ccagctatgg tgttagtatc agaacaatct attagaatgg
    62521 cagaaatgca gtcaagattt gctggaatgg atttaggaat gaattttgaa gaagaaaaaa
    62581 ctcttgtaat aaatgagaac agtccaataa tcaaaaaatt ggtttctctt aaagatgatg
    62641 aggaaaagaa agataagatt acgcttattt gtaaccagat agcagattta gctttacttt
    62701 ctaataaaga attaaagcca gatgagttag attcatttgt acaaagaagt aataagctta
    62761 tgagtctttt gatagaatta taagattgaa tttgaataca ttgaagcaaa tttataaaaa
    62821 gtattcaata tcagtgataa gaaagaagtg tctcaaaatg aacttttcag ttcatgaggc
    62881 gcttttttta tgtgaaatta aatttatttt aatcatttca acaatgaaaa atatatttat
    62941 ctctattttg agacagaccg ttctttattt ttttatcttt ggttaatttt taaatattta
    63001 agtaggttct aataaaaaac tctatttagt taaatatagt taacttgata tataataaga
    63061 taatacatat aattaagtaa aagattgttt tttagtttat gtttaaatac ataattacac
    63121 tttgttaaaa acataaaatt attactaaag tagaaatagg aggttttagc atgaatttta
    63181 attataattt gccagtaaat atcttatttg ggagcggcag aattaatgat gtgggaaaag
    63241 aagtatctaa atatggcaaa aaagtattga tagtaacagg taaaaatagt acaaagaaaa
    63301 ctggattact tgataaaaca atagatttac taaaagactc taaaatagaa tatgaagttt
    63361 ttgatagagt agaacaaaat cctcttataa ctactatata ttcaggagta gaaattataa
    63421 aatcaactgg atgtgattgt gttttaggtc ttggtggtgg aagcattatg gatgctgcca
    63481 agagtatagc tttttcttat aaaaacccag gagatttaaa tgattatata tttggaatta
    63541 gacaaggtga agaagcttta cctataatct tagttccaac aacatcaggt acaggtagtg
    63601 aaggaaattg ttttgcagta cttacaaatt cagaaactaa agataaaaaa tctataaaga
    63661 agaatagtat gtatgcaaaa gcatcaataa ttgacccaga acttatggtt acaatgccaa
    63721 aacatataat tgcatcagtt ggatttgatg cacttgccca taatatggaa gcatatcttt
    63781 ctaatggaag aaaccctctt gcagatgtac aagctattta tgggattgaa cttatatcag
    63841 aaaacctaat aaaggtatat aatgatgtta atgatttaga agcatgggaa aaaataacac
    63901 ttgcaagtac tattggagga atgacgattg gtacagcggg tacagccttg ccacatggaa
    63961 tggagcatcc tgtaagtggt cttagagatg taatgcatgg aaaaggactt gcagctttga
    64021 ctcctactat tatagaaagt tcatgggata gtgatattga aagatttggt aatatttcaa
    64081 aggtattagg aggaacttgt gcagaagatt gttcagatgc aattagaaat ttccttaaaa
    64141 aaatagattt agatataaag ctaagtgatt taggtattga aaaatcagat atagattggt
    64201 taactgataa ttgtatgaaa atatcagttg caaatattaa gagacatcca aagtatttta
    64261 ataaggaaca aataaaggaa atgtaccata agtctttata atttgacttt acatatttag
//
LOCUS       DAYAOY010000010        62120 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYAOY010000010 DAYAOY010000000
VERSION     DAYAOY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.010"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            89..1285
                     /locus_tag="ACGI6B_000709"
     CDS             89..1285
                     /locus_tag="ACGI6B_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFG9750175.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1378..1902
                     /locus_tag="ACGI6B_000710"
     CDS             1378..1902
                     /locus_tag="ACGI6B_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFG9750176.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1927..2103
                     /gene="rpmF"
                     /locus_tag="ACGI6B_000711"
     CDS             1927..2103
                     /gene="rpmF"
                     /locus_tag="ACGI6B_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFG9750177.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2310..2867
                     /gene="fapR"
                     /locus_tag="ACGI6B_000712"
     CDS             2310..2867
                     /gene="fapR"
                     /locus_tag="ACGI6B_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HFG9750178.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2878..3900
                     /gene="plsX"
                     /locus_tag="ACGI6B_000713"
     CDS             2878..3900
                     /gene="plsX"
                     /locus_tag="ACGI6B_000713"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFG9750179.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3926..4912
                     /locus_tag="ACGI6B_000714"
     CDS             3926..4912
                     /locus_tag="ACGI6B_000714"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFG9750180.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5021..5950
                     /gene="fabK"
                     /locus_tag="ACGI6B_000715"
     CDS             5021..5950
                     /gene="fabK"
                     /locus_tag="ACGI6B_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFG9750181.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5966..6916
                     /gene="fabD"
                     /locus_tag="ACGI6B_000716"
     CDS             5966..6916
                     /gene="fabD"
                     /locus_tag="ACGI6B_000716"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFG9750182.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6916..7665
                     /gene="fabG"
                     /locus_tag="ACGI6B_000717"
     CDS             6916..7665
                     /gene="fabG"
                     /locus_tag="ACGI6B_000717"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFG9750183.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7719..7943
                     /gene="acpP"
                     /locus_tag="ACGI6B_000718"
     CDS             7719..7943
                     /gene="acpP"
                     /locus_tag="ACGI6B_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFG9750184.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7972..9210
                     /gene="fabF"
                     /locus_tag="ACGI6B_000719"
     CDS             7972..9210
                     /gene="fabF"
                     /locus_tag="ACGI6B_000719"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFG9750185.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9436..11154
                     /locus_tag="ACGI6B_000720"
     CDS             9436..11154
                     /locus_tag="ACGI6B_000720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9750186.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11185..13185
                     /locus_tag="ACGI6B_000721"
     CDS             11185..13185
                     /locus_tag="ACGI6B_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750187.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13536..13781
                     /locus_tag="ACGI6B_000722"
     CDS             13536..13781
                     /locus_tag="ACGI6B_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFG9750188.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13816..14541
                     /locus_tag="ACGI6B_000723"
     CDS             13816..14541
                     /locus_tag="ACGI6B_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFG9750189.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14563..15876
                     /locus_tag="ACGI6B_000724"
     CDS             14563..15876
                     /locus_tag="ACGI6B_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9750190.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16082..16621
                     /locus_tag="ACGI6B_000725"
     CDS             16082..16621
                     /locus_tag="ACGI6B_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9750191.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16710..18695
                     /locus_tag="ACGI6B_000726"
     CDS             16710..18695
                     /locus_tag="ACGI6B_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFG9750192.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18919..19887
                     /gene="spoIIIAA"
                     /locus_tag="ACGI6B_000727"
     CDS             18919..19887
                     /gene="spoIIIAA"
                     /locus_tag="ACGI6B_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HFG9750193.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19877..20398
                     /locus_tag="ACGI6B_000728"
     CDS             19877..20398
                     /locus_tag="ACGI6B_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HFG9750194.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20415..20612
                     /gene="spoIIIAC"
                     /locus_tag="ACGI6B_000729"
     CDS             20415..20612
                     /gene="spoIIIAC"
                     /locus_tag="ACGI6B_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HFG9750195.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20637..21014
                     /gene="spoIIIAD"
                     /locus_tag="ACGI6B_000730"
     CDS             20637..21014
                     /gene="spoIIIAD"
                     /locus_tag="ACGI6B_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HFG9750196.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            21099..22304
                     /gene="spoIIIAE"
                     /locus_tag="ACGI6B_000731"
     CDS             21099..22304
                     /gene="spoIIIAE"
                     /locus_tag="ACGI6B_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HFG9750197.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22364..22981
                     /locus_tag="ACGI6B_000732"
     CDS             22364..22981
                     /locus_tag="ACGI6B_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HFG9750198.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22993..23547
                     /locus_tag="ACGI6B_000733"
     CDS             22993..23547
                     /locus_tag="ACGI6B_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HFG9750199.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23574..24263
                     /locus_tag="ACGI6B_000734"
     CDS             23574..24263
                     /locus_tag="ACGI6B_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HFG9750200.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24373..24735
                     /locus_tag="ACGI6B_000735"
     CDS             24373..24735
                     /locus_tag="ACGI6B_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFG9750201.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24820..25326
                     /gene="nusB"
                     /locus_tag="ACGI6B_000736"
     CDS             24820..25326
                     /gene="nusB"
                     /locus_tag="ACGI6B_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFG9750202.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25310..26380
                     /locus_tag="ACGI6B_000737"
     CDS             25310..26380
                     /locus_tag="ACGI6B_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HFG9750203.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26377..27582
                     /gene="xseA"
                     /locus_tag="ACGI6B_000738"
     CDS             26377..27582
                     /gene="xseA"
                     /locus_tag="ACGI6B_000738"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFG9750204.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27584..27799
                     /gene="xseB"
                     /locus_tag="ACGI6B_000739"
     CDS             27584..27799
                     /gene="xseB"
                     /locus_tag="ACGI6B_000739"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFG9750205.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27802..28689
                     /locus_tag="ACGI6B_000740"
     CDS             27802..28689
                     /locus_tag="ACGI6B_000740"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFG9750206.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28719..29156
                     /locus_tag="ACGI6B_000741"
     CDS             28719..29156
                     /locus_tag="ACGI6B_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HFG9750207.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29190..31055
                     /gene="dxs"
                     /locus_tag="ACGI6B_000742"
     CDS             29190..31055
                     /gene="dxs"
                     /locus_tag="ACGI6B_000742"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFG9750208.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31188..32000
                     /locus_tag="ACGI6B_000743"
     CDS             31188..32000
                     /locus_tag="ACGI6B_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFG9750209.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32020..33699
                     /gene="recN"
                     /locus_tag="ACGI6B_000744"
     CDS             32020..33699
                     /gene="recN"
                     /locus_tag="ACGI6B_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFG9750210.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33913..34779
                     /locus_tag="ACGI6B_000745"
     CDS             33913..34779
                     /locus_tag="ACGI6B_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750211.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34872..35363)
                     /locus_tag="ACGI6B_000746"
     CDS             complement(34872..35363)
                     /locus_tag="ACGI6B_000746"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9750212.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35424..35864)
                     /locus_tag="ACGI6B_000747"
     CDS             complement(35424..35864)
                     /locus_tag="ACGI6B_000747"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9750213.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36025..37083
                     /locus_tag="ACGI6B_000748"
     CDS             36025..37083
                     /locus_tag="ACGI6B_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFG9750214.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37225..38049
                     /gene="spo0A"
                     /locus_tag="ACGI6B_000749"
     CDS             37225..38049
                     /gene="spo0A"
                     /locus_tag="ACGI6B_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HFG9750215.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38462..39583
                     /gene="steA"
                     /locus_tag="ACGI6B_000750"
     CDS             38462..39583
                     /gene="steA"
                     /locus_tag="ACGI6B_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HFG9750216.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39601..40476
                     /locus_tag="ACGI6B_000751"
     CDS             39601..40476
                     /locus_tag="ACGI6B_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HFG9750217.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40500..41192
                     /locus_tag="ACGI6B_000752"
     CDS             40500..41192
                     /locus_tag="ACGI6B_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9750218.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41200..42024
                     /locus_tag="ACGI6B_000753"
     CDS             41200..42024
                     /locus_tag="ACGI6B_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFG9750219.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42029..43096
                     /locus_tag="ACGI6B_000754"
     CDS             42029..43096
                     /locus_tag="ACGI6B_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HFG9750220.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43098..43634
                     /locus_tag="ACGI6B_000755"
     CDS             43098..43634
                     /locus_tag="ACGI6B_000755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9750221.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43702..44307
                     /locus_tag="ACGI6B_000756"
     CDS             43702..44307
                     /locus_tag="ACGI6B_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HFG9750222.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44319..45233
                     /locus_tag="ACGI6B_000757"
     CDS             44319..45233
                     /locus_tag="ACGI6B_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9750223.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45275..46447
                     /locus_tag="ACGI6B_000758"
     CDS             45275..46447
                     /locus_tag="ACGI6B_000758"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFG9750224.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46494..47306
                     /locus_tag="ACGI6B_000759"
     CDS             46494..47306
                     /locus_tag="ACGI6B_000759"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFG9750225.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47510..48835
                     /locus_tag="ACGI6B_000760"
     CDS             47510..48835
                     /locus_tag="ACGI6B_000760"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFG9750226.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49048..50127
                     /gene="mltG"
                     /locus_tag="ACGI6B_000761"
     CDS             49048..50127
                     /gene="mltG"
                     /locus_tag="ACGI6B_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFG9750227.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50299..50973
                     /locus_tag="ACGI6B_000762"
     CDS             50299..50973
                     /locus_tag="ACGI6B_000762"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFG9750228.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50960..52207
                     /locus_tag="ACGI6B_000763"
     CDS             50960..52207
                     /locus_tag="ACGI6B_000763"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFG9750229.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52279..53949
                     /locus_tag="ACGI6B_000764"
     CDS             52279..53949
                     /locus_tag="ACGI6B_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFG9750230.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54046..54330)
                     /locus_tag="ACGI6B_000765"
     CDS             complement(54046..54330)
                     /locus_tag="ACGI6B_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9750231.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54353..55870)
                     /locus_tag="ACGI6B_000766"
     CDS             complement(54353..55870)
                     /locus_tag="ACGI6B_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9750232.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55995..56816)
                     /locus_tag="ACGI6B_000767"
     CDS             complement(55995..56816)
                     /locus_tag="ACGI6B_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9750233.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57007..58434
                     /locus_tag="ACGI6B_000768"
     CDS             57007..58434
                     /locus_tag="ACGI6B_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HFG9750234.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            58584..58736
                     /locus_tag="ACGI6B_000769"
     CDS             58584..58736
                     /locus_tag="ACGI6B_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750235.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     repeat_region   59390..59682
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=59390..59418
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(59969..60193)
                     /locus_tag="ACGI6B_000770"
     CDS             complement(59969..60193)
                     /locus_tag="ACGI6B_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9750236.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            60904..61059
                     /locus_tag="ACGI6B_000771"
     CDS             60904..61059
                     /locus_tag="ACGI6B_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFG9750237.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            61338..61556
                     /locus_tag="ACGI6B_000772"
     CDS             61338..61556
                     /locus_tag="ACGI6B_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750238.1"
                     /translation="MKILQQFKPYKYDKEKNIEYDEKIIANQNTEVYIVYPRISDEEN
                     RRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            61864..61989
                     /locus_tag="ACGI6B_000773"
     CDS             61864..61989
                     /locus_tag="ACGI6B_000773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750239.1"
                     /translation="MRFEMVEVNIKIKGEVSEIINSIRGIFNSFDEELILDIKSE"
BASE COUNT        24924 a         6413 c        11243 g        19540 t
ORIGIN      
        1 aataaaagat agagttttat ttagacattt attacaaatc aaaatttatt acatattatt
       61 tataaaaaaa tctaaggagg aaataataat gaaaatatta gtattaaact gtgggagttc
      121 ttcattaaaa tatcaattaa tagatatgaa taatgaagaa gttttatgta taggattggt
      181 tgaaagaata ggaatagaag gttctatatt aaaacatgaa aaagcaggta gagatgataa
      241 atacgttgtt gaacaaccaa tgaaggacca caaagatgca atagcattag ttttagaagc
      301 tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa gaaatagatg cagtaggaca
      361 tagggttgta catgctggag aaaaatttgc aacttcagta gtaataactc cagaagtaga
      421 agaagcttta aaagaatgta tagatttagc tccacttcat aatccagcaa acataatggg
      481 aatagatgct tgtaaagcta tacttccaga tgtaccaatg gtaggtgtat ttgatactgc
      541 tttccatcaa acaatgccta aatcttcata cttatatggt ttacctcatg agctatatac
      601 taaatatggg gtaagaagat atggattcca cggaacttct cataattatg tatctcaaag
      661 agcagcagag atattaggaa aagatataaa agatttaaaa atagtaactt gtcacttagg
      721 aaatggtgct tctatagctg ctgttgatgg tggaaaatgt gtagatacat ctatgggatt
      781 cactccatta gaaggattaa taatgggaac tagatgtgga gatatagacc cagctatatt
      841 accattctta atgagaaaag aaggtttaga tgctgatgga ttggataaat taatgaataa
      901 agaatctgga gtatatggaa tgactggaat ttctagtgac ttcagagata tagaagatgc
      961 agctaaaaat ggagacgaaa gagctcaagc tacattagaa gcgtatgtta aaaaagtaca
     1021 aaaatatata ggcgcttatg ctgctgaaat gaatggattg gatgttgtag tatttactgc
     1081 aggcgttggt gaaaatggaa aggctataag agcagatatt gcttctaaca tggagttttt
     1141 aggaatgaaa ttagataaag aagctaatga tgttagagga aaagaaacag taatatctac
     1201 tgctgattct aaagtaaaaa tgcttttaat accaactaat gaagaattaa tgatagctag
     1261 agatacatta agattggtaa aataattaaa ttaaacctat gcctatcaag taaaaatact
     1321 tgacaaacaa atgctagatt tgtataataa atttggtgat atgttgaggt gaacttaatg
     1381 aaagttagca tagaaaaaat aaatagaaaa gaaactgaca aaatagactt gaatttttgt
     1441 gaaaaaattg atactattag ttattgtgat gagatttaca aattagtatc acctgtaaat
     1501 ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc tagatataga tgtcaatttt
     1561 acaattgttg acaattgttc aagatgtctt aaagaagtag aaataccact agagtattct
     1621 atacaaggtt ttttagtaaa agaagaagat tatgatgaag atgagtttga ggaatttgat
     1681 ccttttatat tcgatggtga agaaatcgac cttattgata taatagaaca gacattagat
     1741 tttaatgtac ctcataaagt tctttgtagt gaaaactgta aaggtctttg tcaagtatgc
     1801 ggggcaaact taaatgaaga agagtgttct tgcagtgaaa ttacaaacga tgaggaatac
     1861 atagatcctc gttttgctaa attaaaagat ttatttaatt aaattttgac taaggaggtg
     1921 gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa acacaaaaat gagaagagca
     1981 gctaactcaa agatggaagc aacaggattt gtaagttgcc cacaatgtca tgagccaaaa
     2041 ttaccacata gagtgtgtcc agactgtgga tattataaag gtaaagaagt tgtgtctaag
     2101 taatagcttc taaaaagcat gtagagtata ctctacatgt tttttttgta catataaaaa
     2161 aataagataa tttactagag aaaataagtg gattttatgc atattaaaaa atagaaaaat
     2221 aataatataa gtattgaaaa tttttgtgtg ttactatata ctactattag tagctggtac
     2281 taattacagc tataaaattt ggagggaaca tgaaaaagaa aagtaaagcc caaagacaaa
     2341 aagagcttat agatatgcta aagacagatc ctttttatac tgatgaagaa ttatcaagtc
     2401 tgtttgatgt aagtatacag accataagat tagatagaat gagtctaaac ataccagagc
     2461 taagagaaag agtcaaatca atagcagaaa ctcagagttc aaaagtaaag actttaggag
     2521 taaaggaaat tacaggagaa atcattgatt tatctgtagg gcgattaggt atatctatgt
     2581 tagaagttac acaggacatg atttactcta agactaatac tttaaaagat acgtatattt
     2641 tttctctagc agattcattg gcaatggcta taatagatgc acctaaggtt attatgagag
     2701 ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga taggcttata gcaaaagcag
     2761 aagtgtatag aaatatagat aaaaagcatt atgtaaaagt tgttatcaac aataaagccc
     2821 aagagcaaat atttagagga aagtttatct ttgaagaatt agattaggag ggaaattatg
     2881 aaaatagtaa ttgatggaat gggtggagat aatgcaccta aatctaatgt agaaggcgca
     2941 gtaaatgcta taaaagaata tcaggttgat ttaataatca caggagataa agatttactt
     3001 gaaaaggaat tttcaaatta tgaatttgat agaaataaat tagagatagt tcatactact
     3061 gagattatag aaaacgaaga taaaccagta aaggctataa gaagtaaaaa agattcatct
     3121 atggtagtag cattaaatct tgtaaaagaa ggaaaggcag atgctataat atcagctgga
     3181 aatacaggag ccttacttgc tggtggatta tttgttgttg gtagaataaa aggaatcgat
     3241 agaccttgct tatgttcagc aataccaaat gtaaagcgag gaatgacact tatagcagac
     3301 tgtggcgcaa atgcagattg taaacctaag aatctagttg aatttgcagc tatgagtaat
     3361 atttattcaa gaaaagtttt aggtcttgaa aatcctaagg tagcattagc aaatgttgga
     3421 ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat atgaagaaat taaaaaatta
     3481 gatttaaatt ttatagggaa tgtagaagct agagaagtta taaatgcata cacagatata
     3541 atcatatgtg atggatttac aggtaatata ttacttaaat ctgctgaagg tgtagcactt
     3601 tcagttatga gtcttataaa ggaaactttt atggccagta caaaaagtaa aataggagca
     3661 ttacttataa aggatgattt aagaaaatta aaaagtttta tagattattc tgagtatggg
     3721 ggagctccac ttttaggtct aaatggagga gttataaaag ctcatggtag ttctgatgct
     3781 aaagctataa aaaatgctat aaatcaagga ataaaatttt caaagggaaa agtcgttgaa
     3841 gatattaacc aatttataag caaatacaat gaagaaaata aaaataacga agatgaataa
     3901 actcgttttg gaggtttaat atataatgaa tacaaaggct ggaatattag gagtaggaag
     3961 ttatctacct gagcaatcat atgataattt tcacttcgaa aaaataatgg atacatcaga
     4021 tgagtggata agtactagaa ctggtattaa ggaaagaaga tttgccaaag agtctgaagc
     4081 aacttctgac ttagcatcta aagctgcctt aaaagctata gaatgtgcaa agttaaacgt
     4141 agaggatata gagctgatta tattagctac aattactcct gatatgtctt tgccttcaac
     4201 tgcatgtata gtacaagatg caataggagc agtaaatgct actgcatttg atatatcagc
     4261 agcttgttct ggatttgtat atggagtaac tattgcaaag caatttgtgg aaacaggttg
     4321 ctataaaaat gtgcttgtaa taggagcaga aacttgttct aagtttttaa attatgatga
     4381 tagaacaaca gcagtattgt ttggagatgg agctggagca gctgttattg gccctgtaaa
     4441 tgagggtgga atactgtcta cacatatggg ctctgatggt aaaggaaaag attgtttaaa
     4501 agtacctgct ggaggttcaa gacttaaggc aagtaaagag actgtagaag ctaatctaca
     4561 tacaatagaa atggcaggta gtgatgtgtt taaatttgca gttagaaaga tggcagaaac
     4621 ttctttaaga gctcttgaaa aagcaaatct aaatacaact gatatagact acttagtgcc
     4681 acatcaagct aatataagaa tcatacaagc ttcttctaag agattagagc ttgatatgaa
     4741 aaaagtttat gtaaatatag acaaatatgg aaatatgtct gctgcatcta taccagtagc
     4801 attagatgaa gcttatagag aaggtaaaat taaaaagggt gataatgtag ttttagttgg
     4861 ctttggtgga ggtctaactt ggggggcttc agttgtcaag tggactttat agtataaaat
     4921 atctaaaatg atttaattaa caaaattaaa cttatacaca aaataaagtt gattaataat
     4981 aaaaagttta cttttatgat ttcaagtgaa ggagaattgt atgaataaaa tttgcaaaat
     5041 attaaatata aaatacccag ttatccaagg gggaatggca tgggtagcta ctgcatcatt
     5101 agcaagtgct gtatctaatg caggaggact tggcataata gcagcaggaa atgcaccaaa
     5161 agaagctata aagaaagaaa ttgttgagtg taaaaaatta acagataagc cttttggagt
     5221 aaatgtaatg cttatgtcgc catttgttga tgatataatt gatttgatta tagaagaaaa
     5281 agttcaagtt attactactg gtgctggaaa tcctgcaaag tatatggata gattaaagga
     5341 agctggaaca aaggttattc ctgtagtacc tacaatagct ttggcacaaa gaatggaaaa
     5401 gctaggagct acagcagtaa tagcagaagg tactgaaggt ggaggacata taggagaact
     5461 tactactatg gtcttagttc cacaagttgc tgatgctgta aacatacctg taatagctgc
     5521 tggaggaatt gtagatggta gaggaattgc agcatcattt gcattaggtg ccagtgcagt
     5581 tcaagtagga actagattta tttgcagtga agagtgttct gtccattcaa actataaaaa
     5641 cttagtacta aaagcaaaag atagagatgc aattgtaaca ggaagaagta ctggtcatcc
     5701 agtaagaaca ttaaaaaata aactatcaaa agaattttta aagatggaac aaaatggagc
     5761 tactcctgaa gaactggata aaaaaggtac aggagcttta agatttgcaa cagtagatgg
     5821 agacatagaa aaaggttcat ttatggcagg tcaaagtgct gctatggtaa aagaaataac
     5881 accttgtaag gaaattatag aggctatggt aaatcaagca agagagatta tgccagcaat
     5941 agaactgtaa atcaggagga gcaagatggg aaaagttgca ctagtattcc ctggtcaagg
     6001 agctcaatat gtaggtatgg ccaaggattt atatgaaaat aatgctgttg ctaaaagtgt
     6061 aatagatgag gctagtgatg ccttaaatat ggatttaaaa aaccttatgt ttaatggaaa
     6121 tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgct atggttacac atagtgtagc
     6181 agtattaaaa gcggtacaag ctcaaataga tttaaaatat aatgcatgct taggattatc
     6241 cttaggagag tatagtgcgt tggttgcagc agatgcaatt gactttaaag atgctgtatg
     6301 tttagttaaa aaaagaggta agtttatgca ggaaacagtt ccagcaggtg ttggagctat
     6361 ggcagcagtt ttaggtttag atagaaatgt acttgaaaat gtggttaaag atatacaagg
     6421 aggtattgta gaagtagcaa attacaattc tcctggacaa atagttatat ctggtgaaaa
     6481 tgaaaagata gaagaagcaa tggtaaaatg caaagaagca ggagcaaaaa gggcagttaa
     6541 attaaatgta agtggacctt ttcactcatc aatgttaaaa gaagctggtg taaaattggc
     6601 cagtgaatta gaaaaagtta acataactaa acctaaagtt gatgtggtgg caaatgtaaa
     6661 tgcagattac tataaaaacg aaggtaaaga cctgcttata aaacaagtaa gttcatctgt
     6721 tctttgggaa gattctatag aaagactttt aaatgatgga tatgatactt ttatagaaat
     6781 gggacctgga aaaactttaa aagcatttat taagaaaata gcttccaata aaaaagcaaa
     6841 tgtcaatatt tataatatag atggtataga ttctcttaat gaatttgtac aaaattatag
     6901 aaatggagag atataatgat aaatcttaca ggacaagtag cagttgttac tggtggttct
     6961 aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt ttggagctga tgtagtaatc
     7021 aattatactt ctaaagaaga tgaagcacta aaaactaaaa atgaaataga aagtatgggg
     7081 gtaaagtgta cctctataaa atgtgatgtg tctaaatttg atgaagtgaa tcaaatgata
     7141 gattctgttg taagcgaatt tggaaaaatt gatatattgg ttaataatgc aggcataact
     7201 aaagatggtc tgcttatgag aatgaaagaa gaagattttg atagagttat agatataaac
     7261 ttaaaaggtg tctttaattg tacaaaagca gttactaaac ctatgatgaa aaagaagtat
     7321 ggaagaataa taaatatgac ttcagtagtt ggaattatgg gtaatgcagg gcaaactaat
     7381 tattgtgcat caaaagcagg tgtaattgga tttacaaaag cttctgcaag agagttagca
     7441 tcaagaaaca taaatataaa tgcagtagca cctggattta tagaaacaga tatgactaaa
     7501 gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca taccaaagaa atcttatggt
     7561 aaaccagaag atgtagccaa tgccgtagca tttttagtta gtgacatgtc aagttatata
     7621 acaggacaag taataaatgt agatggtgga atggtaatgc aataataatt taaaaagtag
     7681 agaaatctat taaaataaaa aattttagga ggtacacaat gtttaataaa gttgtagaaa
     7741 taataataga gcaattagga gtagaagata aagaaataac tatggaaaca tcattaatga
     7801 aggatttaga agcagattca ttagatgcag tagaaataat aatggcactt gaagatgaat
     7861 ttggtataga aataccagat acagaagcag aaaacttcaa atctatagga gatatagtta
     7921 agtatataga agctaataaa tagtcgagct ttaaaaaagt ggaggtatcc gatgaataaa
     7981 agagtagtaa taacaggact tggatgtgtt acgccgttgg gaacaggaaa agaagagttc
     8041 tggagtaata taaaatctgg agtatctggt atagataaaa taactaattt tgatgctagc
     8101 acatatcaaa ctcagatagc aggagaagtt aagaattttc atccagaaga atatataagt
     8161 aaaaaggaat tgaagagatt agataaattt gctcaatttg caatagtatc tgctaaatta
     8221 gcagttgaag atgcaaatct tgatttagat aaagttgata gagaacgttt tggtgttata
     8281 ataggttctg gaataggtgg agttgaagct atagaaactc aacataaaat tcttttggaa
     8341 aaaggaaata aaagagtaag ttcacttttt gtacctatga tgataggaaa tatggcagct
     8401 ggtcaagttt caatattttt aggagcaaaa ggacctaaca ctaatgtatg tacagcttgt
     8461 gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa ttcaaagagg tgatgcagac
     8521 cttatggtag cagggggttc tgaagctgct gtaacaggtc ttgcttttgc tggattctgt
     8581 aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa ctgcatcaag accatttgat
     8641 aaagacagag atggttttgt aatgggagaa ggagcaggta ttgttatctt agaagactta
     8701 gaacatgctt tagcaagagg tgctaaaata tatgcagaag tagttggata tggtttaact
     8761 gctgatgcat accatatgac aactccagct gaaaatggag agggagcagc taggtctatg
     8821 aatatggctt tgaaagatgg aaatgttcca ttagaagaag tagattatat aaatgctcat
     8881 ggaacttcaa cttattacaa tgatttatat gaaactatgg ctataaaaac tgtatttgga
     8941 gaaaaggcat atgacttatg tgtgtcatca acaaaatcta tgactggtca cttattagga
     9001 gcatcaggtg ctatagaggc tgttgtttgt gctatgagta tagaagatag ttttgtgcct
     9061 ccaactataa acatacaaga agttggtgaa gatttggatt tagattatgt tccaaatcaa
     9121 ggtaaagaaa aaaatattag atatgcacta tcaaattctt taggatttgg tgggcataat
     9181 gctacaatag tactaaaaaa atatgtataa ataaatatta aatataaaac taaaaaacaa
     9241 gcacttgttt taactgtata aatttatatt agcttaaatt taaagtctcc taaattattt
     9301 taggagattt ttttattaag atttaagtct aggatatgtt gtaaatcatt ttgatatatt
     9361 gtattattaa ttattaagag gttatttata tatgcaagtt ttcattctac aatatacaaa
     9421 aggaggacta aaattttgaa ttttagacta ccaaagaaaa ggttacccaa aaagataatt
     9481 tttatatgta taatacttat tagtattatt ttgtttataa agtatgacca aaatggattg
     9541 aaagaaagag aaattataaa taagggaaaa tatatgtctg aaagccttgt ggcagataag
     9601 gagtatgatg aagcaaaaaa agtggttaag acttcttttg aatctattcc agaaaaaaaa
     9661 tacaataaag tatataatga agtttgggat atgttaaaga ctatttcata tgttccagat
     9721 ggaacgaaga tagtcattga ttctttagaa aagatgagag aatcagatgt gaaattatct
     9781 gatgaatgta gatttaatat agaaaaaaga ttagcatcag tgtatataat ggataataat
     9841 tattctaagg cagtagattt aactgtaaag tctataaagt tggctgagaa attggggaat
     9901 aattatgaaa aagcaagact agatgttgat ttggcgagca tatttttaaa gataggtggc
     9961 tatgaaacag gagagaaatt aataaaggat tcttttaaaa tagatattga tgaaggtgaa
    10021 aaaaatggaa tggttagatt atatgctatt ataaatctag cagaaatata tgtagaagag
    10081 ggcgaatatg ataaagctat tgaaataagt agcaggataa aagattataa aaagtttgta
    10141 aatacggata attacaatga ttttgatata atggcctcta taatgcagtc aaatgcgtat
    10201 gtgggcaaaa ataatataca aaaggcaaag acttttttag aaaaagctga ttatcttata
    10261 aaatcagata gaacagttta tatattagat aaagatatgt actattatat ggcaatggga
    10321 aacttagaat ataaaagcga aaattataat tctgcaatag ataattataa aaaaagttta
    10381 gagatatcta ctaaaagaaa gttaacccag gagaaaataa aaaatttaaa taagatatta
    10441 aatatatatg aaaaacaagg aaatatagaa tctttaaatg agtatcaaaa gatgttaata
    10501 aatgaatata aagagaacaa aagtataaga gattcagaga ctagtttcta tataattgat
    10561 aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa aagaaataaa atattataaa
    10621 atattattta cagtaatttt aattgctata ttagggtcta cttttttata caaccgattg
    10681 aagtatttta aagcacaaaa tttacatgat ggtcttacaa atgtttataa tagaaaaagt
    10741 tttgatatta tgtatgaaaa gtacagagag aaagatgata attttgcact tgtgatgata
    10801 gatattgata atttcaagct cataaatgat aattatggac ataaatttgg tgacactgtt
    10861 ataaagggaa taacaaatac catttgtgtt atgttagaac aagatgataa agtctttagg
    10921 tacggaggag aagaattttc tgtattaata agaaataaat ctggtgatga agtagcagat
    10981 ataattgaca gtataagggt tgcaattgca aataaaaagt ggaatgatga tgtgactgtg
    11041 actataagtg caggtgtagc tcatgtttca gatagcaaag atgtactaga agaagctgat
    11101 aaaaatttat ataaagcaaa acaacttggt agaaataaag tagtatataa gtaattatat
    11161 actatataaa taagaggtat aaatatgaaa caatcaatag ctatagtaac tgataaaata
    11221 actaaactat catctttctt agaagaaaat atacgtttgg ttttaggtaa ttatattgat
    11281 ataaaccact attatctgga agttttaaaa gagaatgata aaatacaagg tgattttgta
    11341 ctagtaatga atgatgatag attaaacagt atgagaaagc atttagtaca agatagtaag
    11401 attatagtga taaggagaac acttaaagaa aatgaaatat atggattatt ttcaatacca
    11461 aatggaactg aagtattggt tgtaaatgat acaaaagaaa ctaccttaga aacgataagc
    11521 ttattttaca aaataggagt aaaaaattta aagtttactc catatattga aggaaaaatt
    11581 tataaaaata tagacatagc tataacacct ggagtaaagg gaaaagtacc aagttatata
    11641 aataaagtaa tcgaccttgg aaatagatat attgatatat ctacctttat agaaataata
    11701 aatggtctta gaatagatgt taaagaaata cgtaaaaatt taatggcata ttctgagagt
    11761 ttaataagtt tagataatgg agttaaagat aattataggc aactgttttt aaaaatagaa
    11821 gaacaagatg ttatattgaa tctatcaaaa gatggaataa tatttacttc agcagatggt
    11881 ataataaaca catttaataa agaagcttta aaaattttag gtataaatga aaatccacaa
    11941 ggcaaaaaca tagaagatat catttctgaa gatttaaata ttttacttga agacaaagaa
    12001 ataattgatg aagtggtatt tacaaataaa aaatatataa atgtaaataa aaaaagtatt
    12061 ttttctatgg ggaataaagc tgggatatat tataacttac aagaaataac gtatataaaa
    12121 aaattagaac aaaatcttac taataaatta agagaacaag gtcagatagc aagatataca
    12181 tttaaagata taaaaactaa aaataaaaat atggtaaagt gtgtagaatt ggctaaaaaa
    12241 atatctaaat cagacctttc tgtattaata ataggagaaa gtggtactgg taaagaatta
    12301 ttatcacaat caattcataa tgcttccaat agaagtaatc aaccgtttat agcagttaat
    12361 tgtgcagcag ttccagatag tctattagaa agtcaactgt ttggatatga aaaaggctct
    12421 tttacaggag ctttaagaga aggaaaaaag ggtctatttg aacttgctaa taatggaact
    12481 atatttctgg atgaaattgg agatatgcct ccattgctac agacaaagtt actaagggta
    12541 ttacaagaaa agcaggttat gcctataggt tcccataatg ttataaacat agatgttagg
    12601 gtcatagcag caacaaacaa aaacttacta aagatgatta gagaaggaaa atttagagag
    12661 gacctgtatt atagactaaa tgtattgcca atagatgtac catcattgag gaatagaaaa
    12721 gaagatataa taacacttat gaatttcttt ttgaaaaaca atataagact ttcgccagaa
    12781 gttataacta ttttagaaaa atataattgg ccaggaaata taagagagtt acaaaatgtt
    12841 gcttcttatg taagtctaat gtgtgatagt atggttttta gagatgactt accaccatat
    12901 ataagggtag aatgtgctaa caattatgaa gatggatttg gttatacaag aaaagaattt
    12961 gaggaaatac tgaggattct atatgaaaga agagagtcat cctctggaat tggaagaggg
    13021 tttataatcg aaaagcttat acaggaaggt attgagattt ctgaaagcaa agttagaaag
    13081 gtgctatctt atttaagtga atgtggatac atatcatcta aatctggtag aggaggaagt
    13141 caaataacct cagaggggat aaaactttat aataatataa attaagctaa gtatttgata
    13201 ttgaaatcac atattttgaa tttagatatt taaataataa taaactttta tatttataga
    13261 gattaattta tattatgtca gaaagaatat tgtaatttat aattaaatat taataggttt
    13321 ttaaaatagg attagatagg taataggtta agttagaata acccattacc tatttttatt
    13381 tgtaagaaaa gtgtacatag aattagaagt aaaaaataca actaataaaa ctattgaaaa
    13441 tactagataa ataataaata aaaataatta tagattttgg catacatatt gctaattata
    13501 gagataagac gacaaaataa ttgggaggtt taatgatgaa atacaaaaca acagggactt
    13561 gtgctacaga aattgaattt gaagtaaaag aaaataaagt tacaaatgta aactttatag
    13621 gtggatgtga tggaaattta aaagggctaa aagtattagt tgaaggcatg aatatagaag
    13681 atgtaataca aaagttaaaa ggaatagagt gtaaaacaaa gccaacatct tgcccagacc
    13741 aactgtctct agcattggaa aattatatga atataaagta atacataaaa caatcgatta
    13801 gttaggagtt taattatgaa accaataata gggattttag gaaatttaat aataatggaa
    13861 aatggtatgt ttccaggtct tgaaaggtca tatgtaaata atgattatat aaatgcagtg
    13921 ctaaaaggtg gcggtagtcc agtaataatt cctgtaaata cagataaaga agttataaaa
    13981 aaacagattg aaatggttga tggagtctta atatctgggg gatgggatat caatcctcaa
    14041 ctgtatggag aagaaacaag agaagaaacg acatttatat atccagaagt ggatgaattt
    14101 gatttaattg caataagtat agcattagaa cttaaaaagc ctatattagg tgtatgtagg
    14161 ggactacaaa ttttaaatgt aagtttagga ggaactttat atcaagataa taatcttata
    14221 gaaggaagtt atataaaaca tacacaatct tctaaaagac atgtagctac tcataaagtt
    14281 gatgttaaag aaggaagtat tttagaggga attttaggaa aacaattact tacaaatagt
    14341 tatcatcatc aaagtgtcaa tcagcttgga aaaggattaa aagccatagc atactcaaaa
    14401 gatggtataa tagaagctat tgagaaagag gatgaaaatt ttgtagtagg agtacagtgg
    14461 catccagaaa tgatggtaga ttactgtgat aaaatggaaa aactatttaa atattttata
    14521 aatatttgtt cgaatttata atataccttg gggggattga acatgaacga aaagaacaaa
    14581 atgggattaa taagtattat acttttaggt attaatgcag tagtaggagc tggagttttt
    14641 ctattaccag gagatgcaat gaaatcattt ggtgtagcaa gtatatttgt atatatattt
    14701 gatatgttgt tagttttatc tatggcattc tgttttgctg aggttgcagg taaattcaat
    14761 aaaaatggtg ctgcctatgt atatacaaaa gaagcttttg gagatttttg tggttttgaa
    14821 gtaggtctta tgaaatgggt aataggatgt atttcttggg gagctttaat agtaggtttt
    14881 cctacatcat tatcagcagt atgggctcca gcaggtgaac cacatataca aaaaattata
    14941 attgtagcta tgatagttgg gcttacaatt ataaacttat taggtgtatc tctatcaaaa
    15001 attgtacaaa atgttataac tgtaggtaaa ctaattccat taatactatt tataggaata
    15061 ggtatatttt ttataaaagg agttaatttt acttcttcaa caatggtacc tccaggagct
    15121 ggagcaacag agtttggagc agcagcactt ttaatgtttt attcatttac aggatttgaa
    15181 tctatagcag ttgcagcaga agatatggaa aatccacaaa aaaatatacc aatagcaatt
    15241 ataagtgtaa tagtaattgc atcaataata tatatattaa atcaagttgt atgtgttggt
    15301 atactaggag attcgttatc tagtacatct acaccagtgg cagatgctgc aagaatatgc
    15361 tttggaaata tgggagctgg attggttacc tttggaacat tggtttctgt tggtggaatt
    15421 tgtatgtgtg gtgcttttgt aaatccaaga agttgtgtag ctttagctga tgataaaatg
    15481 ttaccgagaa tatttgcaag aaaagataaa aaaggtacac catatgtagc gataatagct
    15541 actatgttaa tcactatacc aatagctcta tctggaagtt ttgctgaatt agcagcaata
    15601 agtgcagttg ctagattcat acaatacata ccaacatctt tatccgtatt agtgtttaga
    15661 aagaaaagac cagaattagt aggtactttt aaaactcctt ttggagcagt gattcctcta
    15721 attgctgtat gtgttggaac atggttgtta ttccaagcat ctatgcatca attaattatg
    15781 ggattaggag ctttggcaat tggagtacca ttatacttta taatgaagtc ttataataga
    15841 aaaatctatg gtgaaaattt aaagaaaata gtttaattat gttataacaa tttattgagt
    15901 taaaattaga cattggcaga aaattaaata caaataagta tctacaaggc tatattaaaa
    15961 tattattaat tttttatttt aatatagcct tatattgtta tcaataaatg aaaaatattg
    16021 taacaacaga agaacaatag tattatgttt atagacaaaa aacttattag gaggtaaatt
    16081 tatgccaaca ataacattaa aaaatggtgt agacgtactt attagagaag gtgtaagaga
    16141 agatgctcaa agcattatag atttttataa tgaagtgggt ggagaaactc attttctgtc
    16201 atttggaaag gatgaatata aaatatcttt ggaggaacaa gaaaatgcta tagaatcagc
    16261 aaaagcatct gataattctg ttaaactgat tgcatttata gatggtgaaa tagttgggat
    16321 tgcaacgatt gattctaatc aaaaagcaaa aggaaagcat gttggagtat taggaattgt
    16381 tgtaaaagaa aaatattggg gaattggatt gggtaaaagg cttatgttag accttataga
    16441 atggtgtaaa tcaaatggaa taaccaagaa gataactctt gtcactaatg aagaaaatta
    16501 taatgctata ggattataca aaaaagttgg atttgaagta gaaagcatac taaagaaaga
    16561 atgttattat aatggtgttt atactgattt aataggaatg tcacttttat tgggaatata
    16621 attagtgtac attttatctc gaaaaaagta tattaagtat ggtataatta aaaaagaata
    16681 taacttaata taattttgga ggacgaatta tgaaaaatct gtgtaaaata tcagatgatt
    16741 atttaaacag caagcttaag tatttaaagt tattatcaaa acaatatcca agtatatcaa
    16801 aagcaagtac agaaataata aatttagagg ctatattaaa tcttccaaag ggaacagagc
    16861 attttataac agatgtacat ggtgaatatg aaccatttgt acatgtatta aaaaatggtt
    16921 ctggtgtaat aaagagaaaa atagaagaat tgttttcaaa tacaataaga gacagcgaaa
    16981 aaaaaatgtt agcaactctt gtatattatc ctgaacaaaa gttagaccta ataattaagc
    17041 aagaggaaaa tatagatgat ttttatagaa taaatattta tagacttata gaactttgta
    17101 aatatgcttc tagcaaatat actaggtcta aagttagaaa actgttacca gaaaatttta
    17161 aatacataat agaagaactt ttgcatgagc atgttaaaag tgaacataaa gaagagtatt
    17221 ataaaagtat agttgaaaca atagttgaca taggaatagc taaggagttt ataattgcta
    17281 tttctacagt tatacaaaaa ttggttgtag atagacttca tgtaatagga gatatttatg
    17341 atagaggtcc aagaccagat ataattgtcg ataagcttat tgaacatcat tgtgtagata
    17401 ttcaatgggg aaatcatgat atattgtgga tgggtgcagc ctcaggtgaa aaaacttgta
    17461 tagcaaatgc actgaggata tcagctagat atgcgaattt agacattgta gaagacatat
    17521 atggaataaa tctattgcct ttagccactt ttgctataga aatgtataaa gatgaccctt
    17581 gtaaagaatt tattccaaag gttaatgacc aaagtgtaac aacaacagaa aagtctttaa
    17641 tggcaaaaat gcacaaagca ataagtataa ttcaatttaa gcttgaaggt gaggttatca
    17701 gacgaagacc tgaatttgaa atggaacata gactcttgct taatatgata aactatgatg
    17761 aaggtacgat taatttaaaa ggtaaaacat ataaattaaa agatacttat ttacctacaa
    17821 tagacaaaaa agacccatat aaattaacta tggaagaaag aaatgttatt gataaattag
    17881 tatcttcatt tagaggtagt gaaaaactac aaaaacatgt ttcatttttg ttttctaaag
    17941 gaagtatata tttaaaggct aactcaaatt tactgattca tggatgtgta ccgttaaatg
    18001 aagatggaag ctttatgtct atgaatataa tgggaaaaga gtataaaggt aaggctctta
    18061 tggataagat ggagtctcta gcaagagaag gatttttctt taaagataag gcagaagaaa
    18121 aactgtatgg catggatatt atgtggtatt tatggactgg gaaatgttca tcattatttg
    18181 gtaaagatga tatgactaca tttgaaagat actttatagc tgaaaaagag actcataaag
    18241 aaaataagaa cccatatttt aaacttagag aaaatgagat ggcttgtaaa agattatttg
    18301 aagaatttga tttagaactt gatgaatctc atataataaa tggtcatgtt cctgtggaga
    18361 gtaaaaatgg agagagccca ataaaagcta atggtaaaat tctcgttata gatggtggtt
    18421 tttctagggc ttaccaaaaa actactggta tagctggata cactttgata tacaattcta
    18481 gaactttgca attagtatcg catgaaccat ttaattctgc tgaagaagcc attgcaaatg
    18541 aaagtgatat cttatcgaca actgttgtag tagagcataa ggcaaagaga aaaatggtga
    18601 gagatactga tgaaggaata aagatacaag aggaaataga agatttaaaa ctacttttaa
    18661 tggcttataa gaaagggcta ataaaagaaa tgtagatgtt atagaggcac ttcatgattg
    18721 aagtgtcttt ttgttaatac ttattaaata tattataaag attaataata gtagtttata
    18781 ttatacttac taagtgaatc caagataaat gggttcactt tttttattta taaacattat
    18841 taaaaaaata tagatgtttt ggttatattc cttgtccttt ataaatagaa ttatataaac
    18901 aaaggaggag gacaagagat gaataaactt tctgatgaaa taataaactc tctttctaca
    18961 actataagag aaaaggtaga aaaagtctca aacaataatc tgaacataga agaaattagg
    19021 ttacgctcac aaaaaccatt gatactaaat gcaaactcta aagattattt ttacaatcaa
    19081 aaaactatga cacttgattt aaatcaacaa aactcatatg tagtaacaag agaagatgta
    19141 gagcaaactt ttcaaataat atgtaaatat tcaatacatt catttatgga tgacataaaa
    19201 aaaggattca taactttgag aggaggtcat agagtagggt tagttggaaa agcaatagta
    19261 gaagatggac aagtcaaaaa tataaagcat atatcatctt taaatataag ggtatcaagg
    19321 gaaattattg gatgctcgga taagatttta agtcatatca taaagggaaa aaatcagatt
    19381 aataatactt tgataatatc accacctcaa tgtggaaaaa caactttaat aagagatata
    19441 gtaagaaatt taagtaatgg taatgaagat tatggattta aaggtttaaa agtggcttta
    19501 gtagatgagc gtaatgagat agcaggagct tatctaggtg ttccacaaat ggatgtggga
    19561 ataaggactg atataataga aacttgccca aaagacttgg gtattacaat gcttttaagg
    19621 tctatgtcac caaatgttat tgtgacagat gaaattggaa gtgaaaaaga aataaaggca
    19681 ttatacacag cattaaatgg aggtatagga ttaataacta ctgtgcatgg agattcaata
    19741 gaggatatac aaaatagaaa agaattaaat agacttctgg ataaagagtt atttaaaaag
    19801 gttataattc tttctgcaaa aagaggagca ggaacaattg aaaaaattta tgatttagaa
    19861 gaaaagagat ggtattttgc aaattaaaat aattattata gctttcttga taggaagtag
    19921 ttatttgata ggtgaacaaa tatacaaaac ttatactcgt agacataaac aattaaacga
    19981 tttaataaga gttttagaaa ttttgaggat ggatttatct tttggacttt atacattaga
    20041 agaaatcttt aataggatag gtggaaataa agagttttgt ttttggaaat ttttctatca
    20101 aatatcagaa ggacttcaca atgaacaaag taaaactttg gaaataataa tatcagaaaa
    20161 tattgatgtc ttaagtaaag aaacttatct aggcaataaa gaaattgagg aactgaaaaa
    20221 tctaatactt actttaggga aaagcgatat agagtcacaa caaagaatga tagatttatc
    20281 tatagaaaat ttgaaaaaac aaacctatga gacaaaggaa gatataaaca aaaaaggcgt
    20341 attgtataaa aaattagtga cattcatagg tataggtata tgtattatat tgatttaata
    20401 tggggaggtt tattatggaa atatcattga tattaaaggt tgcaggtgtc ggtattctaa
    20461 tatcagtatt gaatatgata ttggaaaaaa cagatagaaa agattgggca ggtcttacta
    20521 cattagcagg tgtaataatc gtattgggta tggtaataac agaaataagt gacttgttta
    20581 atactgtaag aacaatgttt caactttact aaagaggagg gagtaatttg gaaataatgc
    20641 aattaatagg aattgcaatc atttcaacta cactctgttt agtaattaaa aaagatagac
    20701 cagagatagc aaatttcata gctataataa ctggagtaat aatactattg tcagttatgt
    20761 ttaagctaaa ttttatagta gatagtatac aagatttggc taacaaggct aacatcccaa
    20821 ctatgtatat atcattaata ataaaattaa ttgggatagc atacttaatg gaatttgcga
    20881 tacaactttg taaagattgt ggggaaggaa acattgcttc aaaactagaa tttggaggta
    20941 aaataatagt tatgtcaatg tctttcccta tacttttgtc cattgtagaa atggtggtga
    21001 atataattcc atagatgtgt atcttggaat gaatataagt atattagaga gctttcgttg
    21061 tattgaggtt tatagagtga agagaggcat ggttgcttat gaaaaaaaga tttttaccaa
    21121 tgattatagg ttttttattt actttctttt ttgtaaatat ctttgctatt atgatatttg
    21181 ctaatgaagt accaagtagt gaagataaag aatatggtga aacaaaaaat agcatagata
    21241 agtatataga tggtcaatta gacaagctag atataaatga gattcaagac tatataaata
    21301 aagaaatagt tattaatgat gttaatttaa aatcatttgt aaaagattta attagtggag
    21361 aaaaaaatat cttagattta ttcaataaag atggattgaa gatattgatg tttgatgaat
    21421 ttaaagcaag tttaaaggta gtagcagtaa ttttggtact ggctctgtta tcatctattt
    21481 taaagagtct ggaaaactct ttttcatcag gtgcagtaag tcaaatagca acatatataa
    21541 tatttatcac aatggtatca cttacactta ttggttttaa agatgtatta cagatttgct
    21601 atgatgcaat agaccatact gtaggtctta tgcaagttat aatgccaata ttaataacat
    21661 ttttactttt aattggattc ccaataacgt cgactacttt gaatcctatc ttcataggag
    21721 gagtaacatt tataaatgta ttttttaaaa attttctatt tgtatctatt actgttgcat
    21781 ttggaatttt aataataaac aatttatcaa aaaatataag gctaaagaga tttttctcct
    21841 ttgtaaaaca aataaattat gtttctattg gagctatgtt tactgtttac ttaggtcttg
    21901 tatccataca aggattgtat gtaaccagtt ttgataaatt cagtgtaaag actgctaaat
    21961 ttgctatagg taattttatt cctgttgtag gtggatttgt atctgactct gtggatattt
    22021 tattatcttc atctcagctt ataaaaaata tatttggagg aataggactt atcttacttg
    22081 ttggaatttg cttacttcca gtaataaaaa ttttatcagt aatagtagtg tacaaattag
    22141 cagcaataat tgtagagcct gttggagagg atggaatatc taatttttta aatgaagtgg
    22201 caaatttaat gataataatg ctagcatcag taattgctat aacagtaatg ttctttgtaa
    22261 ctgtagctat cttgacctcg ataagtgtcg taagtcaagg gtaatttata tatcagtgaa
    22321 aaataattac atttaatcta atgtttttag agaggaggat gctatgttag agggcataaa
    22381 ggcatggata gttagtgttt tgataggagc atttatagta aatatagtag atatgatttt
    22441 accaagttct aagataaaac cttatgtcaa cctagtttta aactttatgt ttgtatttat
    22501 tgtaataact ccagtagtag gatttttttc aaaagatatg agtttagaag atagaatatt
    22561 aaaatctatg ggaaactaca acaagcagta tgtagatagt acaaatgctt tggcaaaaga
    22621 gactggaaat aatagtctat ccaagggata tgaggatggg ttaaaagaag tacttaaatt
    22681 aaaacttgat gaatatggtt atgatttaga agatatagag cttaatgggg caaatataaa
    22741 taatataaaa ataaaagaaa agaataatag tgcaaaaagt aataacagtt ccaatgaaga
    22801 aaataaaaat aatagtacta aaagagagga agaaaacata aactctaatg acaaagaaaa
    22861 ttctaaacaa gtcttcaaaa aaggtacaga gtatgggttg aacctaaatg aagaaaaatt
    22921 aaaaaacgac ttaataaagg tgctggatgt ttctatagaa gatatacaaa ttgataaata
    22981 ggagttggga gaatgttgaa aaacttaaat gacaaagata agagaaaggt atattcacta
    23041 ataactatag caggaatatg tgtggtttca ttagtattat tatcttgttt tcctagtagc
    23101 aaaagcgata aacaagttgg aaaaacagag actaataaaa atgctgaaaa gcaagtcact
    23161 aaagaacaag aaaaagatga tttagagtca aaattaacag caatactatc taaaattgat
    23221 ggtgcaggag acgttgatgt aatggttaca tttgaatcaa gtgaagaaat acaacctgct
    23281 tttaattcaa ataatacaac agaaacaaca gaggaaaaag atgcacaagg tggagaaaga
    23341 acagttacga cttctagtga aaataaaaca atgataactt caaattcaag tgaccctgta
    23401 gttataaaaa ctacagagcc aaagataaag ggagtgatag tggtagcaag tggagcaagt
    23461 gacccaagtg ttaaagagac actttatagt gcagtacaaa catcattaca agtagctggt
    23521 catcaagtag aaatatattc taaataaata catataaccg gggggataaa tgaatgaagt
    23581 ttaattataa gggaagagga tttgtaataa taactttaac tgcaatgtta gtggtagtag
    23641 gaacagtgaa ttatcaacta agtaagaaat ctttattaga aacatcaaaa gagtttaagg
    23701 catatgaaga agcacagttg caaaaaaata ctgatgagag tgatagtagt aagacagagg
    23761 attctagtaa tgtagataaa caagatggta aggaaagtgc tgatattgat atagttgata
    23821 gcaaagctag taaagtaaaa gaaaaggcaa ctgagacaag taaggaaata aaagctcaat
    23881 tatcatcaga gaaaaatatg aaaaaggcat cttatatttt agatatgaaa atgaatagag
    23941 aaaaacaaag aaatgagtta gttcaagatt taaatgaaat gataaataat ccatctacaa
    24001 cagaagaatc tagaaaagaa gcttctaata tgaaattaaa tatagttaaa attcaagaaa
    24061 aagaacttca aatagaaaac cttttgagta caaaagggta tgaagaagcg ttagtttata
    24121 taagcgataa caaagttaat gtagttgtaa atgaagctaa attagaaaag aaagatgcag
    24181 caaaaatatt tgatttagtt gcagaacaag ccaatgtgaa gtatgaaaat attaaactta
    24241 caaataataa tagtaataag taaagtataa acctcagaat taactgaggt tattttcttg
    24301 caaagtagac ttgtatttgg tattattata cttataaaaa tagttcctta aaatcaggag
    24361 gtaagtttta atatggaaga taataaattt ggacaagtaa aaatatctaa tgatgtaata
    24421 gctacaatag ctggtttagc agcattagaa gttgaaggca tagaaactac agcaacatta
    24481 acagataagt tgttaaaaaa taatggagta aaaatacaaa tcgaagaaga ggatgttaat
    24541 ttagatgtaa tggttacgat aaagtatgga atgtcaatac cagatacagc ttttaaagtt
    24601 caagaaaatg taaaaaatac tgttgagaca atgacaggac tgaaggtttc tcaagtaaat
    24661 atacacattc aaggaatcag ctttaagaag gataaagttg ataaagaaga agcaaaagca
    24721 actaagaaaa attaatatta gccctctgaa aagtcagagg gttttttgct ggtaattgat
    24781 tttatatgga taattaaact taaaggagaa ttttgattga tgaaaaaaga tagggcacaa
    24841 aagagtacaa caagagaata tataatgaaa cttatatacc aaataaatat taataaagaa
    24901 gattttgaaa ctttagaaga taaagtagat aattttttaa aagataattc agaacatatt
    24961 ataaatagat ataaagaact tgcacttcaa tactcaaaaa atacaaattt aaagttagaa
    25021 gatactgaaa ttgaagatgt tatagacaaa aaatatataa atacagtatg caaagcattg
    25081 aaagaaaatc atgacaaaat agatgagtta ataaataagc atgctaaaaa ctggacagta
    25141 gatagaatgc caaaggtaga tgtttctata cttagattgt ctgtgtgtga aatactttat
    25201 ttagacactc ctaataaagt atcaataaat gaagcagtag aacttgcaaa aatatattgt
    25261 gatgataagt ctcctaaatt tataaatggt atattgggaa gtgttgttga tgaaattggg
    25321 aaataatata ataattggaa ttgatactag ctgctatact acttctatag cagctatctc
    25381 tttagataag aaagttatat tcaatgaaaa aataatgtta gaagtaaggg ataattcaaa
    25441 aggtttaagg caaagtgaag ctgtatttca acatatcaat aatttaggga ttcttagtga
    25501 taggataaaa tcttttaaag ataagtttaa tgtagaagga gtttgctcat caaaaaaacc
    25561 gagacctgtt gaaaactcat atatgcctgt attcaatgta ggtcataatt ttggaaaatt
    25621 attgtctagt atatatggtt gtaggtttta tgagactact catcaagaga atcatattga
    25681 agcaagttta ttaaatagta aattaaaaaa taataataaa tttatttctg ttcatatgtc
    25741 tggtggtact acagagatac ttttaacaag caaacaagat agtcatcaca atgtatgtga
    25801 tactaattta ggtaaaattg ccaaaataag tattaaaaaa gatgataaaa gtaagttata
    25861 caataatttt ggatataata tagatataat tggaggaagt aaagatatta gctttggtca
    25921 acttatagat agagtaggaa ttaagcttgg atataaattt ccttcaggaa agtatttgga
    25981 tgaaaatgct ttaaattgta atcttaaaat agaaagtggt ctgaaaactt ctgtaagaga
    26041 tgggtatatg aatttatcag gattagaaaa tcaggtaaat aagattataa atgataatgg
    26101 agataatact aatcaaaaag aatatatatc taaattggta ttagattcag tagttagaaa
    26161 tatgtttaaa tctttagtgt atttatgtga aacttataat gtaaatgaag tagtatttgc
    26221 tggtggagtt tctgcaagta agtatattct tagagaatta agcatgaagt taagaaaaaa
    26281 gcatatagaa gcttatttta cagaaccaca atattcaact gataatgctg tcgggtgtgc
    26341 tataatagga ttgaataact ttttaggaga aagagtatga aactaagagc tttagatata
    26401 agtgaagcaa actcatatat taaaagaatt ttaataaacg acccaatact ttctaatctt
    26461 aaagttaaag gagaaatttc taattttaag gttcatagta gtgggaatgt atatttatca
    26521 cttaaagatg aaacatcaaa gttaaactgt gtaattttta aaagtaattt taatcgcaat
    26581 ttaaaattag ataatggtgt aaaaattata gctaatggat atatatcagt ctatgaaaga
    26641 gatggtgcgt atcaattata cattaatgaa attgaaatag agggaatagg aaatttacac
    26701 atagagttta atagattaaa ggaaaaactt aataaagaag gattatttga ccctaaatac
    26761 aagataccta tacctaaaat gccaaactca attggcgtta taacatctcc aacaggtgct
    26821 gttataagag atataataaa tgtaataaaa agaagatatc cgaaagttaa tataaagctt
    26881 tatccagtaa cggttcaagg agataaatca gctgaagaaa tctgtgaagc aattagattt
    26941 ttcaatcata tgaagaatgt tgatacactt atagttggta gaggtggggg ttcgatagaa
    27001 gaactttggt cttttaatga tgaaatggta gcaagagagg tttttaattc tcaaattccg
    27061 ataatatcag cagttgggca tgaaacggat tttactattt gtgactttgt gtctgatatg
    27121 agagccccaa cgccatcagc agcagctgag attgctactc catcattgga tgatataaac
    27181 tataaacttg gaaacataaa aagtaggatg agtaagtctc ttacaaatca aatagagcta
    27241 gaccaatata ggttagaaac agtatttaat aagataaata actatttaga ttcgtacaca
    27301 ataaaagata aagttataca attagacaaa atatatgata aaataatttt tggaatagaa
    27361 aataatttaa aattagaaga tgaaaaatta gtaaaaattg gagcattact ccataattta
    27421 agtccattag ctactatgga tagaggttat agtataactc aaaaaaatgg aaaagtaata
    27481 aatagtataa aaggattaaa aataaaggat agtatagata tagtattaaa agatggaaat
    27541 ttagaatgta tgatagataa aattgaaaat aaagagggat agtatgaatt taacctacga
    27601 agaagcttat aaaagattag aaagtatttt aagtgaatta gaatctaaaa atgctagctt
    27661 ggatgaatct ttaagcctat atgaagaagg tataagcctt tataaacatt gtaataagct
    27721 tttagatgat gctaaattga aaataagtaa atttaatcaa ttgggaatag aagaagactt
    27781 taaaatagag gaggaataaa attggaattt aaacaatgtc taaaagaaaa agcaagtttt
    27841 gtagaaaaag tacttaaaga atatatgcct aaagaagaag ggtatcaaaa aactgttatt
    27901 gaagctatga actatagctt aagtgctgga ggtaaaagat taagaccaat acttacattg
    27961 gaggcttgta aaatagttgg aggtaatgaa gatgaagcta ttccttttgc tattgcaatt
    28021 gaaatgatac atacatattc actaatacat gatgatttgc cagctcttga caatgatgat
    28081 ttaagaaggg gaagaccaac aaatcataaa gtatatggag aagctatggg gattttagca
    28141 ggagatgctt tgttaaacta tgcttttgaa gtaatgcttg caggctcaat caataaagaa
    28201 aatccagaaa agtatctaaa agctataaat gaaattgcca aaggtgctgg tatatatgga
    28261 atgataggtg gtcaagtagt agatgtagaa agcgaaaata aacaaattgg aaaagaaaaa
    28321 ttagattata tacatatgaa taaaactgct gcaatgatgg taggctgtat gagagcagga
    28381 gctactatag gtggagcaaa ttctgaacaa atggaagaaa taactaaata tgcaaaaaat
    28441 atcggattat cattccagat agtagatgat atactagaca tagtaggaga tgaagctaag
    28501 ttaggtaaaa aggttggaag tgatatagaa aatcataagt caacataccc atctctttta
    28561 ggtttagata aatctaaaga aattgcacat aatttgattg atgaagcaaa gaaaagtata
    28621 gaaaaattat ctgatgatgt agatttttta aagggattgg ctgaatatat aatagataga
    28681 gaatactaaa atatacagct aaaatcgagg agggaaaaat ggattttttt tcggagattt
    28741 ttaacaatgg ggctttggga ataagtctga ttgcttgttt cttagcacaa tttataaaaa
    28801 tatttactgg taaggaaaaa agaattgagt tatcaagaat acttatttca ggaggtatgc
    28861 ctagttccca tagttctttt gttacaagtt tagctacagt ggtaggtatt gaaaaagggt
    28921 ttaattctac tgattttgct ataattaccg tgcttgcctt aattataatg tatgatgcag
    28981 caggtgttag acgggctgtt ggtaaacaag ctactatatt aaatcaaatg gtggcagata
    29041 tacagcatgg aaagcatata gagcagaaga aattaaaaga attaataggt catacaccac
    29101 tagaagtttg gtttggagct ttacttggaa tagtaacagc tttaatattg atgtaaattg
    29161 aaaatttaca gaataaaggt gaggataata tgtataaata tttagataaa gtaaattctc
    29221 caaaggatat aaaaaatatg agtatagaag aaatggattt acttgcaaag gatataagaa
    29281 agttcttagt aaaatctgtt tcaaagacag gaggacactt agcttctaat ctaggagtgg
    29341 ttgagttaac attagcactt cataaagtat ttgatagccc taaagataag attgtatggg
    29401 atgtagggca tcaatcttat gttcataaaa tagttactgg aagaaaagat tgttttgtat
    29461 ctttgagaca gtttaatgga ctaagtggtt ttccaaagga gaatgaaagt cctcatgata
    29521 tttttgatac aggacatagc agtacatcta tttctatagc tacaggtatt gcctgtgcta
    29581 gggatataaa aaaagaaaac tatagtgtta tatctgtgat aggagatggt tctataacag
    29641 gaggtatggc acttgaagct ttaaatcaat taggatatat agataccaat atgatagtaa
    29701 tccttaatga taatgagatg tccatagata aaaatgttgg aggaatgtct aaatatctgt
    29761 caagtattat aagaaactca acagttgaga aaatgacaga tgaagttgat aaaattttaa
    29821 atgttacaca aacaggtgaa attttatcta agactgcaca tagatttaaa gataaactaa
    29881 tgtatagctt ttctcctcag gactgttcat tttttgattc attaggtata agatattatg
    29941 gacctataga tggccataat actaaagaac taatagatat attgagaaaa gctaaacaca
    30001 aaaaagggcc tgttctttta catgtaatta cgaaaaaagg aaaagggtac agatttgcag
    30061 aagaacaacc tgataaatat catggagtat caaagtttga tataaagaca ggtgttacat
    30121 ctgcaaaagt taagtctatg tcaattagtg ttggagaaaa acttgttgat atggctaata
    30181 gtaatgaaaa tatagtagca ataacagctg caatgccatc tggtacaggg ttaaacctgt
    30241 ttgaaagtgc atatccaaaa agatattatg atgttggtat agctgaacaa catgcaacag
    30301 gatttgcagc aggtcttgca aaaaatggta tgaaacctta ctttgctgtg tactcatcat
    30361 ttttgcaaag ggcatatgac caagttattc atgatgtatg cataacaaaa aagccagtta
    30421 cttttcttat agatagagct ggattagttg gaaatgatgg agaaactcat catggtatgt
    30481 ttgatttaag ttacttaaat tctattccaa atattgtagt aatggctcca aaagacacaa
    30541 gagagatgga gcttatgatg gatttgtcat taaaattaga ttgtccacta gcaattagat
    30601 acccaagagg aagtagttat tacctagata agggagagta tggagagatt gtactgggta
    30661 aatatgaggt attagatgac ggtcaagata cagtcatact ttgtattgga agtatggtaa
    30721 aacatgcttt agaggctaaa gaaatattat caagagaagg aataaatccg acaatagtta
    30781 atgctagatt cttaaagcca attgatgaag gtatgttaaa agcattatta aaaaatcata
    30841 aaaatgtagt tactatagag gataatattg taactggagg atttggaagt agaataaata
    30901 agtttattat agataatgaa tataacgtaa atatacttaa tatagcaata ccagaagaat
    30961 ttgtaaaaca tggcaatata gatgaattat atgattttgt aggattatcg ccaaaaagta
    31021 ttgctgataa aataaggaaa ttagttattg agtaaaaatc taatgtagat attgtaaatt
    31081 aaaatttata taaaatatag aatttatcaa ttagaataaa tatcaagtgt gttacttcaa
    31141 gctactttag ttatcattaa agtagctttt aacagaaagg taattgtatg aaaaaaagaa
    31201 tagatttatt attagtagaa caagggtact ttgaaagtag agaaagagcc aaaaaagcaa
    31261 taatggctgg attggttttt gttgataatc aaaggtgtga taaggctggt acagaagtaa
    31321 aagaagattg tagtatagaa gtaaaaggaa atccaatacc atatgtcagt agaggtggtt
    31381 taaagcttga aaaagcaatg aaaaactttg atttgactat tgatggaaag gtatgtatgg
    31441 atataggggc atccacaggt ggttttacag attgtatgct caaaaatgga gcaataaaag
    31501 tattttctat agatgttggt tatggtcaac ttgcatggaa attgagacaa gatgataggg
    31561 ttgtgtgtat ggaaagaacc aatataagaa atgtgacaat agaagataca aagcagtttg
    31621 ctgattttgc gtcaattgat gtgtctttta tatcgttaaa gttagtactt ccaaaggcaa
    31681 aggagttagt acgtcatgat ggggaagttg tcgctttaat taaacctcaa tttgaagcag
    31741 gaagagaaaa agtaggtaaa aaaggagtag ttagagaaaa atctacacat atagaagtta
    31801 taaaaatgat ttctgatttc tcagtcgaaa atggatttga gattttaggg ttagattttt
    31861 ctcctattaa aggtcctgaa ggtaatatag aatatctaat tcatttgaga aatggaaatg
    31921 aaggatatga atttgatggt gagacataca ataacaaaat agtagaagtt gttgaagctt
    31981 ctcataattt agacaaatag gttaaaaggg ggcttagatt tgatcctcga attatatatg
    32041 aaaaattgtg ccttagttga agaactaagg ttgaatattg ataaaaactt aaatatactt
    32101 acaggtgaaa ctggttcagg aaaatctata ataatagatg cacttggact ttgtttaggt
    32161 gataaatatg ataggtcttt tttaagaaaa ggaacagata agggacttgt tgaggctgta
    32221 tttttttcag acaatagata tcttaaaaaa atattagaag aaaatgatat aagtatggaa
    32281 gatgataatt tattggtaat aactaggttg atatactcag atggtaagag tactgctaga
    32341 gtaaatggaa ggactgttaa agtgtctctt cttaaagaaa ttgcatctac acttatagat
    32401 atacatggtc aacatcaaaa tcaagctttg tttaataaag atactcattt aaaatttcta
    32461 gacctttttg gagaaaatga attggaagag tttaaaattg cttataaaaa tgtgtaccat
    32521 aagtatagtg aagtaaaaaa agctttaaat tgcttgacag aaaataaaga tgagatgcaa
    32581 attcaacgag aaatagattt attaagattt caaataaatg aaattgaagc tgcaaatcta
    32641 aataagaatg aatatgaaga tttattaaaa caaagagaag tttatagaaa tagtgaaaag
    32701 atatataata atctaaattc gagttatagc aagttacata atggtgagta taatgttatt
    32761 gacctaatag gtcttgcatc taaggaactt aatgacatat ctaaatatga cagtgtttta
    32821 agtgaatata gtgatactgt agaaagaatc atgtatgagt tacaagacat atctggtgag
    32881 ataagaaatt acaaagataa tatagacttt gaaccttatg agctagagca gattgagctt
    32941 agaatcgacg agattaataa cttaagaaga aaatatggag acagtataga tgatatattt
    33001 gagtattatg gaaaaattaa agatagatta gatgaaatat taaacagaga tgaaagagtt
    33061 gaacagttaa gaagcaaact gatgaatata gaagaagatt taaaaataaa agccagtaaa
    33121 ttgactaaag caagaataga agtagcaact actttagaaa aaatacttct tgatgaatta
    33181 aaaagcttaa atatgaaaaa tgtaatgttc aaggttaatt ttgaaaaagg tccatttact
    33241 ttagatggag tagatgatat tgaatttatg atatctttta acttaggaga agatattaag
    33301 cctatatata aagttgcatc aggtggtgag atgtccagat ttatgttagc gtttaaaacc
    33361 atattggctg atatagatga tattgataca ttagtgtttg acgagataga tactggaata
    33421 agtgggatag cagcacagat agttggtgag aaattgagtg atatagcaaa gaaaaaacag
    33481 ataatatgta taactcattt acctcaaatt gctgcaaatg cagacactca ttattgtata
    33541 gagaaagata cttcaaataa taggacgttt accaatgtta gtaagttaaa tgaatctcaa
    33601 cgaaaaaatg aaattgccag acttatagca ggtaacaata taacagaaaa aactattgaa
    33661 catgcaagtg aaattattga gttggctaaa aaatgttagt gaaaaacaag cattttaata
    33721 ggtgtaaaga atttttatag aaatgcttta catctatttt ttatgatgaa attattttaa
    33781 aatatctagt gcctatattg aagaaaagta gatttaaaaa tagaagaaaa tttgtataaa
    33841 tttgtaactt tgaaatttct aaatgcttaa gtataatatt atttattaag gtagtagtga
    33901 gggggacata aaatgaataa aaaagatact atgcacttaa tttttacaat tgtaattttt
    33961 attttattat ggtatattgt ggtagcagta agtgagatta ataaaaatat tgaaagttgt
    34021 agtaataaat tagattattt gtcacaagag attatgagta ctaaatcgga tattagtaat
    34081 actataaatg aagaaatgtc aaaaagttat ataacaaaga gtattgactt aaaagtaaaa
    34141 aaaatcgaga aaaaagagtg tgttattgat gtagatgttc aattaagtaa acttggaaaa
    34201 aatggaaaag tattttttat gtataaagaa gatagtgata aatggaatga aactgagatg
    34261 acaaaacatg gagaattatc atatgcatgt gaaattaaga taaatacagg aagtgaatat
    34321 gactataaag tagttacaag tggagctata tctgaaagtt ctgatgtgca aaaattgact
    34381 aaatctgatt atatgcctga acaacccata tttaattctg ggataagaga taatagagaa
    34441 tactttattg aaatagtaga aaatagtaat cttttcaatc atgagagtga aaagtcaaaa
    34501 aataaagttt atgataatat tagattagaa aaagtagata ttattgtaaa tgaaggaaaa
    34561 aaagacacta tatacaaagc caaattagct gaaggcttag atgatggcaa aactaataat
    34621 tcaaatagaa atcaagtgaa ttatgaagta agttttccta aaagggatat tgatgacata
    34681 atttatatta aagctaaact tacatacaat aatggagtag aatatacaaa ggatataact
    34741 gaagatataa ctatgggatt acaagattta gaaaaataaa tacatattta aataaatatt
    34801 caaatagatt tgttcaataa aatagagtat aaataaaact gtagtagttt tacttatact
    34861 ctattaaaaa attataattt taattccata tatctagccc cttcaattgg attataagta
    34921 taaggttcta tatcataaaa accaaacttt tcatataatc cttgtgcact tttcatgctt
    34981 ggaagagtat ctaatctcat gtgtgtatat ccaaattttt tagcttcttc aatgatttct
    35041 tccaatagta tttttcctat ttttaatcct ctaaattgat ttcttacata taatcttttt
    35101 aattcacata ttttaccttc aagttttttt aaagcaacac aacctgccaa attttcatca
    35161 acaaatgcca atatcaatga gcctgaaggt tttttatatt ttcctggaag tgtttttaat
    35221 tcattattaa agtcttgaaa acataagtct atatttagag aatttgaata ttcagtaaat
    35281 agaagtttta cgttttcaag gttttctaaa tctttttctt caacaaattg taaagttata
    35341 ttttgagcca ttttatccgc cattatataa acatgtttat ataatgactt cacctccttg
    35401 atatatgtat atttttctta atatcaatta gattttaata ccattacttt ttcaccacca
    35461 tagtcaagct ctccattaaa ctcaaatcca aaatctttat aaagcttaat agccatttta
    35521 ttgtcttcga atatactaag atatagttca tcacagttat attcattttt aagatgattt
    35581 acaagaaaat ctaatgatgc ttttccatat cctttacctt gatattttga atcaatcata
    35641 aatctatcaa gccaaactct atcatcttct aaacgaccat acatagcaaa tcctattaaa
    35701 gaattttcat catatattcc aacaggtacc caagctgtta taaatttaga ttcagcaata
    35761 gaaagtgcat tgctttctat ataatctaac tgacttttat ctacagacaa actaactaaa
    35821 tcatgccaat tatttatgtc tgcaattctt acatttaatt tcaagatatt accgcccttc
    35881 aaactttttg ttacaatcta atactatcat attatgtatt aaaaatcact tattaagatt
    35941 ttaaataaac tttttaaatg aataaattct caggaaaaag gaattataaa tacataataa
    36001 tacagcatag gagtagatgg atatatgaac aaaataaata aaaatttcaa aaatatttgg
    36061 ataatattag gagctgtatc gatttttacc atggttataa tttttaataa aaattgtaat
    36121 gttaataaag agactcaaat agaagctttt acaattaaga atcatgataa aaaatttgta
    36181 tatcctatgg gaaatataat tggagtaaaa gcaaatacag atggtgtatt ggtcttagga
    36241 tatgaggaag aggatgtaga ctatattgga ggcatacaaa tcggtgataa tatagtaaaa
    36301 atagataata aaaggataaa aaatagtcaa gatgtatcag agattttgaa taagattaaa
    36361 aaaagtaaag ttgaagtgac ttttgagaga aaaaataagt ataaaacaga agtaatagag
    36421 actaaaaaag aaaatggcaa gtataaactt ggattatggg taagagataa aatatctgga
    36481 attggaacta tgacttttta cgaccctagt atggaaaagt tcaaagcaat cggtcatgcc
    36541 ataaaagatt cggatactaa tgaactatta aagataaagc aaggatatat atataagcca
    36601 gaacaattaa aaatagtaaa agctagtaat gaaaaagtag gtaaaataaa aggagatttt
    36661 aatgacagca atttaatggg taatttctca aataattcag agttaggtat aagtggtaat
    36721 attacagaaa accataataa agagtttaat gtagcaaata aagaaaagca attaatagaa
    36781 gttggaagac cgcaagatgt aaaaatagga gatgcagtaa ttctttttga ggataaaaac
    36841 aaaaatataa caagctatga tataaaaata gaaagtatag tatatgataa aggaaattat
    36901 agagatatgg taataaaagt agtagatgac aagttattgg aatacacagg aggtatcgta
    36961 caggggatga gtggagctcc aataatacaa aataataaaa ttattggtgc aataactcat
    37021 gtttttagag ataatccgaa aaaaggatat ggtattttta tagatgaaat gataaaattg
    37081 taggtgaggc attaaaaaat tttattattt tatcaattat ctaggaggaa tataattttg
    37141 gagtgtcgaa tatgctttag agtagataat taggaagcaa ttgtgtaaaa agtttagttt
    37201 tctgtaataa gaagatgttt tttaatgggg ggatttttag tggaaaaaat caaaatagtt
    37261 ttagcagatg acaataagga tttttgtcag gtattaaaag agtatttgtc taatgaagat
    37321 gatatcgata tattaggcat agctaaggat ggaattgaag cactagactt agtaaaaaag
    37381 acacaaccgg acttattaat actagatgta ataatgccac atctagatgg attaggtgta
    37441 attgaaaaat tgaatactat ggatatacct aaaatgccaa aaataatagt actatcagca
    37501 gtaggtcaag ataagataac tcaaagcgca ataaatctag gagcagatta ctacatagta
    37561 aagccatttg attttgttgt gtttataaac agaattagag agctagtttc taatagagtt
    37621 acacaagttg agccaaaacc tagaccagtt caagagactc aaatgactag aagtgatttt
    37681 gttaagaatg taggaaatat agagacagaa atcacaaata taatacatga aataggagta
    37741 ccagctcata taaaaggata tttatattta agagaagcaa taaaaatggt tattgataat
    37801 gttgagcttt taggtgcagt aacaaaagag ttatatccaa gtatagctaa aaaattcaat
    37861 acaacaccaa gtagagttga aagagcaata agacatgcaa tagaggttgc atggagtaga
    37921 ggaaaagttg acacaataaa tcaattattt ggatatacgg tacacaatac taaaggaaaa
    37981 ccaactaatt cagaatttat agcaatgatt gctgataaat taagattaga acatagtatg
    38041 gttaaataaa caagacataa aaagtaaggc tttttaatta aggcattggc tataaatgcg
    38101 tattacaagc agcgaaacgg tataaccact agggttatac cgtttcgcta ttttaataaa
    38161 tataaaaatt ttctttatta tttgcttact atatcaatat aataatttta ttatactatg
    38221 gatatagtat gtgtctttac aagttgtaaa ctgacagtgg tttatttttt aatataaata
    38281 ttgactttga tgcaggtaaa ctttgtattt ttaagcgtat tatggaatat gttaaataaa
    38341 aaaatgatga aatatagtat tgtaaatgcc aaagatgcaa aacaaactta aaacatttat
    38401 tttattgtta agtaatgcta taatataatg tgattttaat aatgatagtg gaggtttaaa
    38461 tatgagagtc gaggccccta taaaagtaga tcgaaaaacc aaaaaacttg ctaaaagagt
    38521 tgaaagtggg gaaatagcag ttataaatca tatagacata gatgaagttg ctgcaaactc
    38581 tttagtagaa gctaaaataa aacttgtcat aaatgcggct ccttctataa gtggtaggta
    38641 tcccaataaa ggtccaggga tattaactga aaataatata ctaataattg ataatgttgg
    38701 agaagagtta tttgaagaat taaaagaagg agaaactata gaagttgtag atggaaagat
    38761 atatagaaaa ggaaaatttt taggtgcagg agaagtttta gacaagtatg aggtttcaca
    38821 ccaaataaag gctgcctatg agaatttagc tgtagaattg gatagattta tagataatac
    38881 tatagattat gctaaaaaag aaaaaggatt tatacttgga gaagtagaaa ttccaaaagt
    38941 gaaaactaat tatgctaata aacatgtatt gatagttgta agaggtcagg actacaaaga
    39001 agatttaagt actatgcttt cttatataga agaggtaaag ccagtattgg ttggtgttga
    39061 tggaggagca gacgctttaa ttgagtttgg atatactcca gatgtgattg taggtgatat
    39121 ggatagtgta agtgatgaag ctttaaaaaa ggctagtgaa atagtagtac atgcttatac
    39181 agatggaaga gcaccaggtc ttaagagggt tgaggaatta ggcttagatg cagttgtatt
    39241 tcctgcgcca ggaactagtg aagatatagc tatgcttata gcctatgagt ataaggctga
    39301 gttaatagta gcagttggta cacattcaaa tatgattgat tttcttgaaa aaggaagaaa
    39361 aggtatggca agtacttttt tagttagact aaaaataggt tcaaagctta tagatgctaa
    39421 aggcgttaat ttgttataca gaagtaaatt aaaaataaag tacatatggg cattaatagc
    39481 aacagcatta tttcctgtgt tggtagttgc atcattgtca ccaggagttc aacagttcat
    39541 acaactaatg caattaaagt ttagagtatt attacagatg taaattttag gaggataaat
    39601 atgcatatta atatgaaata ttatatagtc actatagggg ctatatttat agctttgggt
    39661 ataggcatgt tagtaggatt taatttaaat aataatcaac aattaagtga acaacaagca
    39721 aatataataa atgatttgga tgataagttt aatatcttaa aagaaaaaaa tgataaactt
    39781 gacagtgact tagcaagtgt aaatagagat tatgaagagg cagttaattt tataaataaa
    39841 aatgtagata agattttagc tggttcgctg aatggtaaga gtataggaat aatatctgct
    39901 aatgaaaatg acgattatac aaaaaatata gaagacataa taaataaatc taatggaagc
    39961 attgcattta atataatatt aaaagaaaac ataacaaatc cagaaaagtt aaaggaaatg
    40021 tcaactaaac taggtacaga tgtaaaaaat gcaaatgatg cagttaatta tataattgac
    40081 actcttaaaa aggaagatgc aagtgacatt ttaacatatc ttcaagaact agatgtaata
    40141 aagtttaatt ttattggaga tacatatcta aaatatgatt ccgttgtaat agctggtgga
    40201 aatgatgcaa aagacagtac aaaacagttt gaaaaaatag aaaaatttgt agtttcaaaa
    40261 cttaaatcag agaataaata tttagtagaa gttcaaaata caggagttaa aacttcttat
    40321 gtagaactat attctaaaaa caaagttgca actatagaca atattgatga aggtatagga
    40381 agtatatctc tagcgattct tctacaacaa ggtaatatag ttggtaattt tggtagattg
    40441 gatactgcaa cttcattgtt gccatctatt aaatagaaat aattttaagg agagaaaaaa
    40501 tggattctta tataagtata ataataccag cttataatga agaaagtaga ataaaatata
    40561 cattagaaag catattggac attaagcaaa taaatgaaat aatagttgtg gatgatggtt
    40621 caattgacaa tactgctaag gttgtatctg agctagaaaa tgaaaagatt aaatttttta
    40681 agttagataa aaatagaggt aaaggatatg ctttaaatta tggtatcaag ttagcaatga
    40741 aaaatgcaga tattataggt tttctggatg gagatttagg aagttctgct aaagaagttg
    40801 aaaaattaat agtacctata ttaaatggtg aggcggatgt tacaatagcc aagtttcctc
    40861 ctgcaaagag aaaaggaggt ttgggatttg taaaaggttt agctaaaaat tctgtgtttg
    40921 agatgactgg tgttgagtta gatgcaactt tatcaggtca aagaatattt aaaagagaag
    40981 ttttagaaaa gtttgatgaa ataccttttg gatatggagt agaagttgga atgaccattg
    41041 atatattaaa gtatggattt aaaattaaag aagtattagt aaatatgact cataatgaaa
    41101 caggaagaaa tttaaaaggt tttattcata gagggaaaca gtattatcac ataaaaaagg
    41161 ttttaggaca aaaaaagaaa gaatggaggt aatatcttag tgaaagatta tatactttat
    41221 accattttat tattaactgg attatttgga acctatgctg taattccact atttaaaaat
    41281 ttattgataa atggaaatgt acttagacca aactataaaa aagatatgat accagtaagt
    41341 atgggaattg tatttttgcc tatgattgta atcaatggaa taatacttgc tttctttact
    41401 acagaattta agaatttagc gtatatattt atgtttatgt ttggtatgat atccatgttt
    41461 tttgcaggga tactagatga tgtcattgga aatagagatg taagtggttt aaaggggcat
    41521 tttaaaagtt tatttaaagg aagtcttaca acaggaggtt ttaaagcttt atttggtgga
    41581 tttgtagggc ttatagtttc tgttgctata tcaaaagaca taattgatat aatagtaaat
    41641 acactaataa tagctctatc aactaattta atgaatcttt tggatttaag accaggaagg
    41701 gcaataaaag catatttatt cataatggta attgtatttt taacattgac aggatttgtt
    41761 caagttcttc cattacttat agtaccaaat gtacttgcat actttaatta tgatttaaaa
    41821 gcaagggcta tgatgggaga tactggttca aacgtccttg gaatatctat tggaatgctc
    41881 atttcttttg ggtatccatt taacattaga ttaggatggt taatatttgt agttattatg
    41941 catatattca ctgagaaata ctcattaact aagataatag aaaaaaataa actattaaat
    42001 tttatagata aattgggaag gtagtattat gataagcaaa agagtgggga ttgttgaatc
    42061 aatagtaagt caaactgaaa ctctagatga tataagagta aatataaatg gagaaataca
    42121 aaaggcatat aattatccaa aaatatctgg aactataaat ataggtgacg aagtggtttt
    42181 aaatacaaca gcagtagaac tgagcttagg tacaggtgga taccattttg taataactaa
    42241 tttaaataat atagaatcta ctttaactga aggtggacat ataatgaaac ttagatatac
    42301 gcctcttcaa ataaaagtgg attctgttga ggaacaagaa agtatttatc atgataaatt
    42361 tgcgaatttt aaaggattag aaggtctttc tgttgtagta gggactcttc atagtatgtt
    42421 aactccattt gcagcttcat ttaaaagaaa taatccaaat aaaaaattag tgtatataat
    42481 gactgatgga gcatctcttc caatttattt aagtaaaaat gtggatactt taaaagaaaa
    42541 aaaattaata gatagtacaa taactatagg aaatgcgttt ggtggagatt atgaatgtat
    42601 aaatatatat actgcattaa ttacagcaaa agaaatacta aaagcggatg cagtatttgt
    42661 cagtatggga cctggaattg ctgggacagg aacaaaatat ggatttacag ggattgaaca
    42721 aggctctatt cttgatgctg taaaaaaact tgaaggaaga gcaattgcaa ttccaagaat
    42781 tagttttgct gacaaacgag aaagacatca aggtatttca catcatagta tgactgtatt
    42841 taaagaaatt gtaaatgtaa atgtagacat accaataact atatatgatg atgaaaaatt
    42901 gaactttata aaagagcaaa tcaatttaaa tggtttagac aaaaagcata atataattta
    42961 tatagataat aataaaacga aagaggattt agactatttt aatctaaaag taaagagtat
    43021 gggaagaaat tatgaacaag atgaggcatt tttcaaagct gcaagcacag cagcatatta
    43081 cttaatggag gtttaagatg gtattagaag aaaaaactat tagtagtgat agggtttata
    43141 caggtaaagt aattacttta aaggttgata ctgttgaaat tccagggcaa ggatatcaaa
    43201 aaagggagtt agtagaagtt ggtggagcag taggaatagt tgcaatcaca gatgataata
    43261 aagttgtgtt agttaagcaa tttagaaaac ctatagaaaa gccaatcttt gaaattccag
    43321 ctggtaaact agaaaaaaat gaaagtccaa aagaatgtgc tgaaagagaa ttaaaagaag
    43381 aaactgggta tagtgcaaaa aatattaagc taatacataa atttttcact tcagcaggtt
    43441 tttctaatga gataatgttt gtatatttag caacaggtct tactccagga gagaataatt
    43501 tagatgcaga tgagttttta gatgtttatg aaattgagct tgaagaagca tataacatgg
    43561 tattaaaaaa tgacgttgaa gatgcaaaaa catctattgg attattatta gtaaaagata
    43621 tgtttaaaaa ttaatattta taaaatgaat aaccctttgt cctaaagcat attattttat
    43681 aaaggacaag ggggaattaa attgagaaga acatatagaa aaaatgattt tagagaatta
    43741 aatagacaaa taattattat aggattgttg tttatgatgt ctattgttgt tgggtcttac
    43801 ataaataaaa tattgccagg ttctagtaac aatatattaa acaatataaa tcctgctgta
    43861 gagtattata atctaaatat aagcataaaa gatacagtga tacaaaattt aaaatcagat
    43921 gcaatcttta tgggctcaat agctctatta agtctatttg tagtaacaat tccagcagtg
    43981 ttggtagctt ttgtattaaa agggatgtca attggttata caataaatag ttgtatattg
    44041 gcattaaaat ttaaaagtat aaaaatgatt ttaataatat tatttaaaaa tttgattatt
    44101 attccatgtg caatcatatt agcattaatt agtttaagtt attttaaaga aatggtttat
    44161 gaatttaaaa agaaaagtag aaaaaatatg caatttttaa ttaagaggta tatactaaat
    44221 attataatta taatagcact atcattgggg ttgcaattaa tattaaatac tgcaagtatt
    44281 ggtattatta agttcctagc taaataaagg agtttataat ggatattata gagggatata
    44341 tagattacat aaaagataaa aaaaaattat cagagaatac agtggcatca tactttatgg
    44401 atataaaaaa atatatggaa tatataaatc aaaaaagcat taaactagta gatatagtag
    44461 aaaatgatat tataagttac ttaataaagt tggaaaaaga taatgtatca atagctacaa
    44521 tagcgaggat gatatcctct ataaaatctt ttcatgatta tttattttta aatcatatat
    44581 gtacaaataa tcctgcaaag gatataaaaa aacctaaaat aaaaaaagaa aatataaata
    44641 tacttacaga agaagaaatt gaaagactat taaattttcc aaagttaaca acacctaagt
    44701 tgattaggga caaggctatt tttgaagtat tatatggtac tggtataaaa gtatctgaat
    44761 tagtagagat gaatatagag gatattgacc tagatattga ttatatatat tgtaattcat
    44821 gttgtaaaaa tcaaagagtg ataccacttt gtgatattac aaaactttac ttagaaaagt
    44881 atctaaaaga agcaagacct aaaatggctg cagaaggtga aaaatcatta ttcgtaagtt
    44941 cattaggtca aaggtttaca cgacaaggtc tttggaaagt cataaaaaaa tattctaatc
    45001 tagccaatat agataaaaat ataaatccta caatgcttag acattcattt gctattcatt
    45061 tgttaaatga aggtgctaac attgctgttg taagtaaaat tttaggaaat gttaatttat
    45121 ctagtttgca agtatattta aatcatatag ataaaaacgt gagaagagaa ataaaggaga
    45181 aacatcctag aaatgatgtt gatgtagaat tacaaaaagc agaagctaaa tagactatat
    45241 agcaaatttt ataatttaga aggagcatat ttatatgagc agagtaattt ggattgtaat
    45301 tgatagtgtt gggataggag cattacctga tgctgaaaaa tttggtgata gtaaggatgt
    45361 aagtaccctt ggaaatatat ttaaggaata tccagatatt cagattccta atatgagaaa
    45421 tttaggtata ggaaacatag aaggtataga tttttttgaa agtataaaag aaccaatagg
    45481 ttgttttgga aagtgtaagg aaatgtctca gggaaaagat actactacag gtcattggga
    45541 aatgactggt ataatagttg acaaaccatt taagacattt gaacatggat tctctaagga
    45601 gataatagaa gagtttgaga aaaaaactgg aagaaaagta gtaggaaata aaccagcttc
    45661 aggaactgta ataatagatg agtatggaga gcatcaaata aaaactggag atgtaatagt
    45721 gtatacatca gctgatagtg tatttcagat tgctgctaat gaagaagtaa ttcccctaga
    45781 agaactctat aatatgtgca aaatcgctag ggaaataatg atgggggata atgcagtagc
    45841 tagagtaatt gctagaccat tcataggtaa gaaaaaaggt gagtttgtga ggacttcaaa
    45901 tagaagagat tattcactag acccatttga accaacagtt cttgataata taaaagaatc
    45961 aggacttgat gttttagcag ttggaaaaat agaagatatt tttaatggaa aaggaattac
    46021 agatgctatc cacactaaaa gcaatatgga tggagtggat gaaactttaa attatatgaa
    46081 acaagataat aaaggtctaa tatattcaaa tctcgttgat tttgattcta aatatggtca
    46141 taggagagac ccagaaggat ataagaaagc tcttgaagag tttgatagta gacttcctga
    46201 aataatggct aatatgagag aagatgatat tttaataatc aatgcagacc atggaaatga
    46261 cccaacatat aaaggtacag accatacaag agaatatata cctgttatga tttatggcaa
    46321 taagattaaa aaaggtttta atttaggagt aaaagatact tttgctgata ttggagcaac
    46381 tgttgcagat atactaaatg taaagttgcc taaacatggg tcaagcttta aagaagattt
    46441 attttaaact ataaaatgat aaaaacttag cttaatagga tggagagatt ggaatgtttg
    46501 aaaagatagc acaaagtagt aaatttataa attctaaaag taatataaaa cctaagattg
    46561 gtttgatatt aggttctgga ctaggtgatt tagcaaatga tatagaagac cctgtcacaa
    46621 ttaaatatag tgaaattcca aacttccctg tatcaacagt agcaggacat gctggacaac
    46681 ttgtaattgg aaaacttgaa ggaaaagaag ttattgctat gcaaggtaga tttcattact
    46741 atgaaggata ttctcagaaa gaagctactt ttccagtaag ggttatgaaa gaattaggag
    46801 tcgagattct tatagtaact aacgctgctg gaggcgtaaa taaagaattt aaagcaggag
    46861 atttgatgct tataagagac catataaact ttagtggaag taatccatta gtaggaaaaa
    46921 atgatgacag atttggtgct agatttccag atatgtcaga tgcatattct tctaaatatg
    46981 tagatgtagt taaaaactgc gctaaagaat gcaatataga tgtcaaagaa ggcgtttata
    47041 tgttttttag tggtccaaac tatgaaacac cagcagaagt gagaatggca caaattttgg
    47101 gagctgatgc tgtaggtatg tctactgttc cagaagtcat agtagcttct cactctaata
    47161 taggagttat tggtatatct tgtataacaa atatggctgc tggcatctta aatcaaccac
    47221 taagtcatga ggaagtaata gaaactactc aaaaagttaa atcagaattt atgaatttag
    47281 ttaaaacaac ggttaaaaat ttataagtta aagcttatta tagatttttt gaggaagttt
    47341 ataataaaat agttaatgtt taatttgaaa agaattaata aaaagctgtc ttgatataga
    47401 aagttatttc taataaagga cagttttttt ataatactac aacatttgaa agtgttttaa
    47461 agtttaatat ttatttaaat tattataggt tagaatggag atttcaatta tgagaatgta
    47521 tgatattata aaaaaaaaga gagataacct agagcttagt aaaaaagaga taaactactt
    47581 tatagaaaac tattcaaagg gagatatacc tgactatcaa gcttctgcac tattaatgtc
    47641 gatttattta aataagatga ataaacagga aactgtacat cttacagaag caatgatgaa
    47701 ctctggagac atgataaacc tatctgagat agatggaata aaggtagata agcatagtac
    47761 tggtggtgtt ggagataaga ctaccatagc tcttatacct ttggttgctt catgtggagc
    47821 accagttgca aagatgtcag gtagaggtct tggtcatact ggtggtacta ttgataaatt
    47881 ggagtctata ccaggttttt cgacagaaat ggagataagt aaatttattg attctgtaaa
    47941 taagtctaaa atagcagttt gtggtcaaag tgcaaaggta gcaattgcag ataagaaaat
    48001 atatgctctt agagatgtta cagcaactgt tgataatata tcattgatat catcaagtgt
    48061 aatgtctaaa aagttagctt caggagctga tgcaattgtg ctagatgtaa aaactggtga
    48121 tggagcattt atgaagacat tagaagagtc ttttgagtta gctaaatcta tggttgatat
    48181 aggtactggt atgaatagag atacaatagg gattgtaaca gatatggatg aacctttagg
    48241 ttttgcagtg ggaaactctt tagaagttat agaggcaatt gagacattaa aaggaaatgg
    48301 acctaaagac cttgttgagc tatgcgaagt attaggagca tatatgttgg ttttatcaaa
    48361 ggtagcacat gattttgaag atggtgttga aaaaataaga gaatcaataa aatctggtac
    48421 tgctatacaa aaactcaaag agtttataga gaatcaaggt ggtaataaag atgtagtaga
    48481 tgattattct ttattcaaaa aatcaaagta taaatatcca gttaaatcaa ctaaatcagg
    48541 ttatataagc aaaattaaag ctgaagatgt tggtgtatcg gctatgattc ttggagcagg
    48601 tagagaaact aaagatgata ttttagattt atcagcaggg ataattttag aaaagaaagt
    48661 aggagattat gtaaatgaag gtgaagtttt agcttacatg tattataatg atgaacaaaa
    48721 gctttcattg gcaaagggaa gatttattga ttcctactct attgtagatt caaaaataga
    48781 aaaaaataaa ttaatatatg gtattgtaac taaagaagag attaaaaagt tttagaaaat
    48841 tttagaaaag tgattataaa aggcctaaca tgtcaaaaat aatgttaggt tttttatttt
    48901 ttgataaact ataaattata ccaaaaatat atattttaaa acatttttta caatataaat
    48961 aagtttattc tacaagatga ttgaaaatta aagtatctag ttttataata gaaaatggat
    49021 aaatatacat aaggagtaga ttatattatg aactttaaag aaaatagatt gaaaatagca
    49081 gtattaataa ttgttatttt gattatctta gcaggtatat ttgtgtttat acaaataggt
    49141 ccttatgata aaaataacaa aaaagatgta attatcgacg ttccaagtgg agcatctgtt
    49201 ggaaaaatat ctgacatatt atatgaaaat aaattaataa agaatgaact attatttaaa
    49261 cttttggtaa aggttagcaa taaagctccg tctataaaat caggaacata cttacttaat
    49321 caatcgtatt caaacaatga tataataagt cttttagttt caggtaaaat atatcaggat
    49381 ggtataaaag tgactattcc agagggagct acatctaaag aaattatagc aatgttagta
    49441 agtaaaaatt taggagataa agctactttt gaaaatttaa taaaaaaacc tcaagaattt
    49501 tatgataaat tttcatattt gaaggaagat ggaataacct ctttagaagg gttcttgtat
    49561 ccagaaactt attattttaa ttcaaagaaa caatcagaag aagatatttt aagcgaaatg
    49621 ttaaaagtat ttgacagtaa atacacggac aagtttaaaa agaaacaaaa agaattaaat
    49681 atgactcttc aagaagtaat ggaaatggca tcaataattg agaaagaagc tgttcttgac
    49741 aaagacagac ctataatagc aagtgtgttt tataatagac ttaaggtagg tatgcctctt
    49801 caatcagatg ctacaataca atatatattt gaagagagaa agaaaattgt aacatatgat
    49861 gatttaaaaa ttgattcacc atataatagt tataaaaata agggattacc tcctacacca
    49921 atatcaaatc ctggtataaa atctattgaa gcagcattat atccagagaa aacagactat
    49981 ttatattttg tagctaagat tgatggtgga aataattata gtacaaacta tcaagaccat
    50041 ttaaaatacg ttaaagaata taaagaagca agggataaac aatcaaaaga tacaaaagca
    50101 acaaataaag aaaatacaaa aaaataaacc atgattataa aaagtctaac agggtaagaa
    50161 taatgttggg cttttttatt gaaaattgtt attcttagat ttataataaa gaaagtgtaa
    50221 atatatataa caatatgaat ttaaaaaata ataaattaat ataaaacttg gatatttatt
    50281 attttaggga gtgtttttat gagtaatata gttaatgatt tggttgaaga ttatataaga
    50341 gtgacattaa aagaaaaaga aggattttta aaagatttag aaatttatgc agaggaaaat
    50401 agtgttccta taatacataa agaagtttca gatttattaa aagtcttgtt aaaggttcaa
    50461 aaaccaaaga gaattttaga agtaggatgt gcaattggtt attcttctat tttttttgca
    50521 actattatag gaaaagatgc agatataata actgttgaga gaaatgaaaa aatgatagaa
    50581 aaagcaaaag aaaacataaa attagctgga tttgacaata acataactat actagaagga
    50641 gatgcagaag aaaaacttag tcaagtccaa ggtgaatttg accttatatt tatagatgca
    50701 gccaaaggtc aatataagct attttttgat ttagttatag ataaattaaa agatggggga
    50761 cttttagtat ctgacaatat tttgtataaa ggtatggttg ctcatgatga ttttgttgta
    50821 agaagaaaaa agactatagt taaaagaatg agaaagtatt tagattatat atgtaattgt
    50881 gactatttaa gcacatcatt ggtaccaata ggtgatggtg tagcactaag ttataaagaa
    50941 agtaaaagag gtgatgtaga tggaattaaa taaagtagaa cttttagctc cagcaggtga
    51001 tttggagaga ttgaaaatag ctataacata tggagcagat gccgtttata taggtggaga
    51061 aatttttgga atgaggtctg cagcgaagaa ttttagtaag gaagatatgg cagagggagt
    51121 agcttttgca catgaaagag gcaagaaagt attcgtaaca gtaaatataa tacctcataa
    51181 tgaagatttt ctacaattag aagactattt gttggaacta gaagagatag gaatagatgc
    51241 agttataata gctgacccag gagtgcttag tgtcattaaa aaagtaattc cgaatatgga
    51301 aatacattta agtacacaag caaatacaac caattatcta agtgcaaact tctggtacga
    51361 gcatggaata aaaagagttg tagtggcaag agaattatct tttgatgaaa tatatgagat
    51421 aagagcaaaa acacctttag atatggatat agaagcgttt atgcatggtg ctatgtgtat
    51481 ttcttattct ggcagatgtt taataagcaa ctatatgact ggaagagatg caaacaaagg
    51541 ttcttgtgct caatcttgta gatggcaata tcatttggtg gaagaaaaaa gacctggtga
    51601 gtattttcca atatacgaag atgaaagggg tacattcttc tttaactcaa aagatttatg
    51661 tatgatagag tatataccag agttaatcaa atctggtata acaagtttaa aaatagaagg
    51721 tagaatgaag actgcatact atgtagctac tgttgtaaga gcatacagaa tggcaataga
    51781 tgaattttac agagacccag aaaactggaa gtttaatcca atgtggatgg aagaactaaa
    51841 aaaaggtagt catagacatt ttacatcagg tttttacttg aataaaccaa caacagaaga
    51901 ccaaaattat caatcagcat catatgttag aaactatgat tttataggta tagttagaga
    51961 aacagaagat gaagatggtc ttattgttgt tgagcaaaga aataaaatgt gtgttggtga
    52021 tgaaatagaa gttatgggac catataaaga aactatgttt actaaaattg aagaaatgta
    52081 taatgaagaa ggagaagcta tagaatcagc tcctcatcct agacaaatag tgaagttaaa
    52141 gctatcagta aaagttggta aagattatat gcttagaaaa gttatagaag aaaaggttga
    52201 agaataataa aaaattgata aaaatagtat tagcccctta gaaattgtca ataatgaaat
    52261 tatctaaggg gtgatttcat gtcaaaaaag aagactccat ttttgaaaaa agtgggaaag
    52321 agaagctggt gtatatttac tataatttta ataatttata gtgttttaat ttataggcta
    52381 gttgatatac aagtattaaa aggtgataaa tacaaacaaa gtgttgaatc acaaagcgtt
    52441 gagaaagtcg agttaaatag tggaagagga ataatctatg atagaaacaa caagaagctt
    52501 acagatacaa gtaaatctca ggtattaatt gttgaaaaag aaaaattaaa taataactat
    52561 aaaattttgg agcttattaa gaaagctact aagatgaatg atttagatat atataaagct
    52621 gtacaagagc aattgacacg ccctataata cagattcaga ctaaaaatat cgatatcgat
    52681 aagagtatga aaaaagaact tgagaaaaat ggaataatgg tagaagaaaa aactatgagg
    52741 tacgcaaaag atggtttatt atcacataca ataggatata taaaagaaga tgataaatca
    52801 ggtcaatcag gaattgaaaa gagtatggat agtgtactta gaaattcaaa tgaaaagtat
    52861 ataagtgctt ttaaagctgg agatgcagga aatgaaaaat ctctaaacat actaaaggga
    52921 agtgtaaaaa ctgttgataa taaggataaa gatagacatc taaaaactac tattgattat
    52981 aacattcaaa aaaaattaga acaaatacta aataaagaag aaaatcctac agcagcaata
    53041 atctcggaag catctacagg tgaaattctt gcaatgtgct ctagacctaa ttttgaccaa
    53101 aatgacatat caaaaagttt aaaaggaaaa aatggagaat ttgaaaatag agttataaaa
    53161 gcgacatatc caccagggtc tgtatttaag atggtagttt tattttctgc acttgaaaat
    53221 ggtgttatag atgagaacta tacttacaac tgtacaggta agactaaagt tgggaatact
    53281 aatgaaatac tgcgatgtaa taaaagagat ggtcatggtt tccaaaactt aagacaagct
    53341 ttttctaatt catgcaatcc tgcattttta gatatagcta tgaaacttgg aaaagaaaag
    53401 atactcaaat ctgctgaaaa attacattta tttgaaaagg ttgatattgg tcttgatgaa
    53461 gaaaaaatta gagaagctcc aaaaaacata tcaataagaa atttagctat aggtcaagaa
    53521 aatatagaat ttacaccact tcaaataaat caaatgactc aaataatagc taataatgga
    53581 acattcaaac cactatactt atataaaagt cttgtggaca ataatatgaa tacaataaaa
    53641 acttataagt cttcaaaaaa agaagaactt atttctcctt atgtgtgtac acaagttaaa
    53701 gaatatatga agtctgtatc taggactggt acagctaaag acctaaaaga tattgaaggt
    53761 ggttgtggag ttaaaactgg tacagctcaa agtagtttaa ataaaaaagc tatagaccac
    53821 ggatggataa caggatttta tcctgaagaa agacctaagt atgtaataac tgtgttagta
    53881 gaaggaactc aaaaaggaaa taaatctgca acacctatat ttaaggaaat atgtgaaagt
    53941 ataaaataaa ttatgtacta taaaatttaa ggatatatcg taactttgat tttagataga
    54001 aaattttgtc taatgatgta cgatatatcc ttttaaaata tagtattaac tttcttgaac
    54061 aagctctttt tctagttttt taagagcttt tttttcgatt ctagatacat atgaccttga
    54121 tatatcgagt ttttgtgcaa tttctctttg tgtcttatag ccataaggag ttaacccata
    54181 tcttaactgg acaatttctc tttctctagg agtaagaatt ttgtttagtt gttcgtaaag
    54241 ttttccaact tgaactttta gttcaacttc atcaagaaca tagttagcct ctgtaccaag
    54301 tatatctaaa agacttattt cattacacac tactctttat atgttattgg tattactggt
    54361 cttcatgtaa tttcaaatgg accattaatt caaaagaatc tagtttttgt tctttagact
    54421 tataataaac tgctttatca attacagatt ttaggagttt attttttcca agggaatctg
    54481 aatcatggta aagtgataac acagttttaa tatcttctat tattttagaa ttgtcttttt
    54541 taacagtact caatctattt tttactgtct taattgaatc tttaatttct tcagttcttg
    54601 aacttatatt gttggacctt tcaataaatg tatcttcatc atatattcct cgttctagta
    54661 aatcatataa cttatttttt tgcttgccta attcttttaa ttcagtttct aagcccttta
    54721 agactcgttt ttgaaattct atattactat ctttattatt tttatctaag tctatatcag
    54781 atgcttttaa ttgatatttt tttacagtgt cttccaaaga ttttaaaatg gcagcttcaa
    54841 taaattcaaa tcgagaactt ttattgcaac caggatgata acaaattata tagtcgtggt
    54901 ctgcatatgg tctatatact aatggtctac cacaattttt gcaaatcact attcctgcaa
    54961 gtggattttt tatttgatta ccatagctga catgaccatt ttttttgagt atattttgag
    55021 cttttttcca tgttatttca cttattaaag gttcatgctt tccttttgcc tcaatccatt
    55081 catctttagg tcttagttta actgttttta ttttatttgg atttttagat tttttgtagt
    55141 cctttttttg ccactgaatg tagccacaat atgccttatt ttttattata tttgtaattg
    55201 cagaattgta ccaattagca cctgtagcag ttttaatgcc taagttattt aggtattttg
    55261 atattttact acaacccata tcttcattaa tatataagtc aaagatagtc ctaactacat
    55321 cagcatcttt atttggtaca agtattcttt cttttctacc agttgttaca gcatcatatc
    55381 caaatggagc agaggttcct atgaaattcc cttcttcaac tgattttatt ctacctcttt
    55441 gcatccttct tgatataagt tttaattctt ttcttgccat aaatgcttca aattctgaat
    55501 actcctcatc aaattcatct gttaggtcgt atgtttttct aggtgtgatg attttggtct
    55561 tactttcttt gaatgtttct aatatgatac cttggtcttt catgtctcca cgaccaagtc
    55621 tgtctaagtc cattactaag actgcatcat atttattttc ttcaacttct tttagaagtt
    55681 caagcatttt gggtctatgt attatacttt caccagaaac taattcttct tttatctcaa
    55741 ttacatctaa gttttgttct tttgctagtt ttagtagggt tgatttatgt ctacttaaag
    55801 tttcaaattc gccttgtttt tctgcctctt catcagcacg ggattttctt aagtatatgg
    55861 caacactcat aataagtaat cacactcctt atgcactatt atatagtaaa tcaatagagt
    55921 ttaaaatata gaaaataagc atgtcaaaag ttaatttaac ttttgacata cttattttct
    55981 ataatattaa tgccttataa tcctttaata tcatttagat tagtttttgc atattcatta
    56041 tccgggttaa gttcaagcgc cttattgtaa gattctctag ctttattaaa ttctgattta
    56101 ctcataaaaa taacacctct taaattgtaa gcatcactag cgtctatttt ataagcttca
    56161 ttatttttat ctagatattc taagtattta tcaattgact ttaaagcctc atcatatcgt
    56221 tgtaactcta tataaatata tgattgtgta taatatgcaa gtggattttt atcatcaatt
    56281 tctatagctt ttttgatatc aatagcagct ttattataat ccttatcttc atctttttct
    56341 gtttctgacc tagcgatttt gacataatta gagctaagag ctcttttaaa gtctttatgc
    56401 cataataatt tatattcatt atcttcgatt ataaaatatc tatcaaaatt tgcagtatct
    56461 tctttatttt cagtgatata ctttatagta tcagtttctt ttattttaac aagatttgtg
    56521 tactctttat tagaatattt gtatttctct actttatttt cttcaccaag tttataatct
    56581 tttagggttt gaagtttaaa taaagtatct tgatattctt caaatttttc tagactgaca
    56641 ctttccttac tgtctttact taataaatta tatgctactt cgtagtcttt gatttctata
    56701 ctatcaaggt actttttagc tacttctgta gcactttctt ttggagcaca tccaaccatt
    56761 gatatcataa ttaaaaatga tactaatatt gctgttattt ttttactgat tatcataata
    56821 taatccccct tatattgtct ctcattaatt ttattttatc accatattct accaaagtca
    56881 ttaatttaag agtgatattt ttcattaata ttatattaag taataataac cttttaaatt
    56941 tatggtaaaa ttttcctgaa aagtattata taatttaggt gaaaatattt aagagaagga
    57001 gatattatga aaattaaaaa attattaaca atagggttat ctttatctat atttattgct
    57061 agttgcccta tatctgtaaa tgctcttgat aaaattgaat caattaaagg tgcagataaa
    57121 tacgaaactg ctggtataat tgcagacaaa caaaactata ctacagctat attaattaat
    57181 gcagacagta caatggctga tggattaagt gcaagtggac ttgcaggagc tataaacgct
    57241 ccaattcttt tgactaagaa aaataacata ccaaatgcta cacttaaaag attagaaaag
    57301 gcaaaaaaag tatatataat tggaggagaa aactctattg ataaatatac tgaaactgtt
    57361 ctaaaaggca aaggaataga gattaaaaga cttcaaggta gtgatagaat taagacaagc
    57421 tataatgtcg caaaagaaat aaactcaatt aacaaagtaa ataaagtcat attgactaat
    57481 gcatttaaag gagagcctga tgcaatgagt gctgcacctg tagcagttag agataaagca
    57541 gctatagtat taactgatgg aaaaagtgtt ccatttaata caactggtgt agaaagttat
    57601 gtaataggtg gtacttcatc aatgaatgac aaaatagtta aggatactaa ttcaactaga
    57661 ttaggtggga cagatagata tgatactaac aaaaagataa taaataaatt ttatagtgga
    57721 gtaaaagaat tttatatagc aagtggtact gatttagtgt atgctcttgt tggttctact
    57781 gttgctaaaa ataatgctat tgtgttagtt gataatgata gtaataaaag tgtattgaaa
    57841 agtacaacta aattaactgc tataggaaat ttgagtgata gtatattaca gcagtgttta
    57901 aatgtaacaa aaaatatagg cgattctaat actggaagag aatatacagt agaagatgct
    57961 gctaatgcag tgaaaaaact attaggtgtg aagaaatttt attacgaacc taattattca
    58021 gaagatgaag atttaatatt tctactagat aaaaattttg atttttcagg aaaagagtac
    58081 tatgtatttg cttacattaa acagggtgca gagggtgatg ttagatactg tgtagctaaa
    58141 aatgatatga gcaaagttta tacatatgat gttaatggta atatgaaagt atgtaatcca
    58201 gatgattata aaaataaagg aagtataaca gaggaacaag caaaacagat agcattaaaa
    58261 gaatgtgcta agagacataa agtcagtatc aataacttag tagttgatat gatagatttt
    58321 agagatgata tatatagtgg taatgtctat gctgttaaaa tcaattcaaa aaatagtaat
    58381 ggtacttttg gttggttttt tatagatata gaaactggga aagtttataa ttaaaattag
    58441 aacttttaaa caagtgtttt taattatata atttaccata atatgttata atatttttag
    58501 caagaagatt taacttacaa tctatagagt ggagttcatg tacaaaagat tatcctccca
    58561 acgattcgaa gggaggtgag ttcatggata actttttgtt taatgtttta gctagtttaa
    58621 cagctagtgt ggtagtttac ttaattagta aactattcaa aaaagcaaaa agccactctc
    58681 gcacaaagag tgacttacga gttgaattta aatttatatt taaatccaaa aaataaactc
    58741 tgtttatgat gaactccact ctaacgcaaa atagattgta gttcttcttg cttttattat
    58801 accacaaaac attataaata aaactgttgt ttataaaaaa atgcaaagta aaatttttta
    58861 taaacaatga taaaaatttt attatagagc tattaactta ttaaatatct gtaaatgagc
    58921 agatatttca taagttaata tttattgttt acataaaata tttggtaaaa ataagtaagt
    58981 tttatatgtt ataatagttg tagcaagaat aataatctaa agtggcaagc atgtcagctg
    59041 gtgttatatc agtttatatt tatgataaaa taaaaatctc ccaaacgcca ataagaatga
    59101 tttaaaaaaa taatattttc acttaaaaat aatcactctt tataggagta aattattttc
    59161 ttgattttat tatactacaa attggtacag atatttaaaa ataatatatt cataatatta
    59221 taaatagttt tgcagtgagc gatatttttg ataaaatagg gcttaacagt tgaaacataa
    59281 ggcattgagg gtatatgata aatattatca tttgcactac tcgtggttca ctgcaaattt
    59341 gagagaattg tatagatgta agtcttggaa atgctcaatt tattttgggg ttttatatta
    59401 actaagtggt atgtaaagaa gtgttgatat ttatattgtc tatccccgct agcttgtttt
    59461 atattaacta agtggtatgt aaagtcgtaa attacgaccg tacctttcgt aaaataacaa
    59521 tcgttttata ttaactaagt ggtatgtaaa gctttttgag tatgagtaca agcataggaa
    59581 aattaatagt tttatattaa ctaagtggta tgtaaagaac aaaaaagata catctgtaac
    59641 taatttttat actgttttat attaattaag tggtatgtaa agacagatat aatatcaaaa
    59701 taaggttagg acttttaaaa gcatagccat atcagtaaaa atttaattat catttaaaaa
    59761 ataaattttt atttaaagaa tacacataaa tatttccaga ctcaactctc ttaaactatt
    59821 ttcatatctt ttagtagata cctttgttgt tttttaatct ttagcaactt gttcttgagt
    59881 gaacctctta ttttttctaa gttatttcaa gcttttcaaa aaattctcta tttatattca
    59941 tcacacatat aatacaatta attcagcttt aattgccaat atttagtttt tctgtatctg
    60001 ataaaccaag aatataatca gtatataaac caaaaatttt agcaaatatt attaacccat
    60061 cgtctcttgt tggtctttca ccagactcta ttctattcat aacacttgta tttatatttg
    60121 ttttttcaag caattctttt tgagaactat tcatattttc cctaatatat ttaatccttt
    60181 gatttacatc catacataat tatctcgaaa agtttctgtt ttggaaatat tgttattgaa
    60241 atatagcaat taatgtgaat atatgaaaaa gatacactat gattttataa aaaagaagac
    60301 atacataaaa tttatactta caaagtgttg caaatgagtt aggttttaaa aatgcatcaa
    60361 tttattttaa gtacaggaaa ggggaatatt caattagagt tgatatgtta cctataaact
    60421 atctaagata cttgattatg atatagaaaa tttttttaca aattaaattt ctaagataga
    60481 aatttaatta agaggatgat gaggatgaat aatttacaag taatataaag aaataataag
    60541 agaattttaa atgcatagca actagcagat atatatgaaa atgatgtaaa caatatataa
    60601 actaacttca atagaaataa agagaatgta aattatgttt tattacaatt cgagtatttg
    60661 aaagaattta agaactaatc tattaatagt cagttgattt caaaacattc tagtcaatta
    60721 tacctttgga tcgaaagatg tgcaaataga cactgcaaaa tattagatat tgataaaact
    60781 tgggaacaat ttgacaattt agagaaaata tatttcaaga ttaagcgaga gaaactaagc
    60841 tgtatagaag atgtattaat agaaaattta agatttaagg atacaaatta ataaagcaat
    60901 aatatggcat tagatgtaaa aatagaagtt aaaataataa aagatgttgt tatattaaat
    60961 ataacacatt ggaaaaaaga aacaagtcta ttaacagtta aaatagctag aagtcaaggt
    61021 gatttaagtt atgtaaaata ttttgctaat gagagctaag agcttcttaa caagagtttt
    61081 ggtgtgtctc ttgaaacaag gttaacaatt aataaaagac gaatggcaga agaaggcgta
    61141 tgtaagttta aaagagataa gttaaacaaa ctagatacta tgtaagagga taagaaactt
    61201 atagaagaat atgttacttt tgtaaaggat ttagctttaa aatatagaat ataaagtgat
    61261 tttaagtaaa aattgtaaca attaaataag tataagacaa gtttatgagt tcatatttta
    61321 taacaagggg tgatgttatg aaaatactac aacaatttaa accatacaaa tatgataaag
    61381 aaaaaaatat agaatatgat gaaaaaatta tagctaatca aaacacagaa gtttatatag
    61441 tatatccacg tatatctgat gaagaaaata ggagaagatg ggaagagttc aatgaagtag
    61501 ctaaagaagt agctattaat atagctatga agaaagttga gacagtagaa agttaagaga
    61561 atagagaagt tgctaatact tagcactttc tcatagaaga aaattggaca actattttta
    61621 aataattagg aataaaaaag ttaaatcata tttgaaaaaa ggcgataaaa ttgttgagta
    61681 ttgttattag taataagaat atattaacta tgtattaaaa gaaattataa gtttttagta
    61741 aatatagtat ttaaggatat taaaactggt aaaaaatatc atttagacta ttaaataaaa
    61801 aagtgctggt caaagtagca acacaataaa taaaaaaact tatagaaatt ataagataaa
    61861 aagatgagat ttgaaatggt cgaagttaat ataaaaatta aaggtgaagt aagtgaaata
    61921 attaactcaa ttagaggtat atttaatagc tttgatgaag aattaatatt agatataaaa
    61981 tcagagtaag aatataaagg tagatacgta gaaagttata agtaattcaa gacttaaatt
    62041 aatttctagt ttttttaatg caaaaattta ataaatttca aaatataata attctgacat
    62101 ctaaaaagag ctattaaaaa
//
LOCUS       DAYAOY010000011        61060 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYAOY010000011 DAYAOY010000000
VERSION     DAYAOY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.011"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(101..862)
                     /locus_tag="ACGI6B_000774"
     CDS             complement(101..862)
                     /locus_tag="ACGI6B_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9750240.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1081..1557
                     /locus_tag="ACGI6B_000775"
     CDS             1081..1557
                     /locus_tag="ACGI6B_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9750241.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1655..1807
                     /locus_tag="ACGI6B_000776"
     CDS             1655..1807
                     /locus_tag="ACGI6B_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HFG9750242.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(1967..2155)
                     /gene="rpmB"
                     /locus_tag="ACGI6B_000777"
     CDS             complement(1967..2155)
                     /gene="rpmB"
                     /locus_tag="ACGI6B_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFG9750243.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2516..2866
                     /locus_tag="ACGI6B_000778"
     CDS             2516..2866
                     /locus_tag="ACGI6B_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFG9750244.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2885..4528
                     /locus_tag="ACGI6B_000779"
     CDS             2885..4528
                     /locus_tag="ACGI6B_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFG9750245.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4609..6666
                     /gene="recG"
                     /locus_tag="ACGI6B_000780"
     CDS             4609..6666
                     /gene="recG"
                     /locus_tag="ACGI6B_000780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFG9750246.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6725..7279
                     /gene="rsmD"
                     /locus_tag="ACGI6B_000781"
     CDS             6725..7279
                     /gene="rsmD"
                     /locus_tag="ACGI6B_000781"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFG9750247.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7281..7778
                     /gene="coaD"
                     /locus_tag="ACGI6B_000782"
     CDS             7281..7778
                     /gene="coaD"
                     /locus_tag="ACGI6B_000782"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFG9750248.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7782..8306
                     /locus_tag="ACGI6B_000783"
     CDS             7782..8306
                     /locus_tag="ACGI6B_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750249.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8650..10569
                     /locus_tag="ACGI6B_000784"
     CDS             8650..10569
                     /locus_tag="ACGI6B_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9750250.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            10582..10869
                     /locus_tag="ACGI6B_000785"
     CDS             10582..10869
                     /locus_tag="ACGI6B_000785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9750251.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11002..12261
                     /locus_tag="ACGI6B_000786"
     CDS             11002..12261
                     /locus_tag="ACGI6B_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFG9750252.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            12264..13004
                     /locus_tag="ACGI6B_000787"
     CDS             12264..13004
                     /locus_tag="ACGI6B_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9750253.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            13327..14637
                     /locus_tag="ACGI6B_000788"
     CDS             13327..14637
                     /locus_tag="ACGI6B_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9750254.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            14735..15652
                     /locus_tag="ACGI6B_000789"
     CDS             14735..15652
                     /locus_tag="ACGI6B_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9750255.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            15668..16477
                     /locus_tag="ACGI6B_000790"
     CDS             15668..16477
                     /locus_tag="ACGI6B_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9750256.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            16535..18283
                     /locus_tag="ACGI6B_000791"
     CDS             16535..18283
                     /locus_tag="ACGI6B_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HFG9750257.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            18518..19567
                     /locus_tag="ACGI6B_000792"
     CDS             18518..19567
                     /locus_tag="ACGI6B_000792"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HFG9750258.1"
                     /translation="MNNIIWKITLFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDT
                     VKMQRGINIGNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFK
                     IDEEFFKKIDKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKY
                     DKKLVFELLNEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELN
                     IPKDSNIVVSFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENW
                     ANKNKVKIFLGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIE
                     GTWDRDILSALIEKR"
     gene            19572..21878
                     /locus_tag="ACGI6B_000793"
     CDS             19572..21878
                     /locus_tag="ACGI6B_000793"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9750259.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            21889..22965
                     /locus_tag="ACGI6B_000794"
     CDS             21889..22965
                     /locus_tag="ACGI6B_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750260.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            22983..24401
                     /locus_tag="ACGI6B_000795"
     CDS             22983..24401
                     /locus_tag="ACGI6B_000795"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HFG9750261.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            24416..25624
                     /locus_tag="ACGI6B_000796"
     CDS             24416..25624
                     /locus_tag="ACGI6B_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750262.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(25698..27086)
                     /locus_tag="ACGI6B_000797"
     CDS             complement(25698..27086)
                     /locus_tag="ACGI6B_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFG9750263.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            27427..28761
                     /locus_tag="ACGI6B_000798"
     CDS             27427..28761
                     /locus_tag="ACGI6B_000798"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HFG9750264.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(28903..29820)
                     /locus_tag="ACGI6B_000799"
     CDS             complement(28903..29820)
                     /locus_tag="ACGI6B_000799"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFG9750265.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            30028..30912
                     /locus_tag="ACGI6B_000800"
     CDS             30028..30912
                     /locus_tag="ACGI6B_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HFG9750266.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            31125..32966
                     /locus_tag="ACGI6B_000801"
     CDS             31125..32966
                     /locus_tag="ACGI6B_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFG9750267.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            33271..33978
                     /locus_tag="ACGI6B_000802"
     CDS             33271..33978
                     /locus_tag="ACGI6B_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750268.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            33972..35282
                     /locus_tag="ACGI6B_000803"
     CDS             33972..35282
                     /locus_tag="ACGI6B_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9750269.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            35399..36082
                     /locus_tag="ACGI6B_000804"
     CDS             35399..36082
                     /locus_tag="ACGI6B_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750270.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            36075..38462
                     /locus_tag="ACGI6B_000805"
     CDS             36075..38462
                     /locus_tag="ACGI6B_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9750271.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            38742..39884
                     /locus_tag="ACGI6B_000806"
     CDS             38742..39884
                     /locus_tag="ACGI6B_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFG9750272.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            39903..40427
                     /locus_tag="ACGI6B_000807"
     CDS             39903..40427
                     /locus_tag="ACGI6B_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9750273.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            40527..41345
                     /locus_tag="ACGI6B_000808"
     CDS             40527..41345
                     /locus_tag="ACGI6B_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HFG9750274.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            41423..41734
                     /locus_tag="ACGI6B_000809"
     CDS             41423..41734
                     /locus_tag="ACGI6B_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFG9750275.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(41911..42594)
                     /locus_tag="ACGI6B_000810"
     CDS             complement(41911..42594)
                     /locus_tag="ACGI6B_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750276.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(42687..43337)
                     /locus_tag="ACGI6B_000811"
     CDS             complement(42687..43337)
                     /locus_tag="ACGI6B_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9750277.1"
                     /translation="MSFLGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDG
                     LSRGAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHN
                     KQDIFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMF
                     GFLLNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(43502..45505)
                     /locus_tag="ACGI6B_000812"
     CDS             complement(43502..45505)
                     /locus_tag="ACGI6B_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFG9750278.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(45658..46296)
                     /gene="recX"
                     /locus_tag="ACGI6B_000813"
     CDS             complement(45658..46296)
                     /gene="recX"
                     /locus_tag="ACGI6B_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFG9750279.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            46572..47261
                     /gene="nadD"
                     /locus_tag="ACGI6B_000814"
     CDS             46572..47261
                     /gene="nadD"
                     /locus_tag="ACGI6B_000814"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFG9750280.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            47262..47834
                     /gene="yqeK"
                     /locus_tag="ACGI6B_000815"
     CDS             47262..47834
                     /gene="yqeK"
                     /locus_tag="ACGI6B_000815"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFG9750281.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            47951..48301
                     /gene="rsfS"
                     /locus_tag="ACGI6B_000816"
     CDS             47951..48301
                     /gene="rsfS"
                     /locus_tag="ACGI6B_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFG9750282.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            48655..51075
                     /gene="leuS"
                     /locus_tag="ACGI6B_000817"
     CDS             48655..51075
                     /gene="leuS"
                     /locus_tag="ACGI6B_000817"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFG9750283.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            51928..52596
                     /locus_tag="ACGI6B_000818"
     CDS             51928..52596
                     /locus_tag="ACGI6B_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9750284.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            52636..52716
                     /locus_tag="ACGI6B_000819"
                     /pseudo
     CDS             52636..52716
                     /locus_tag="ACGI6B_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(53079..53351)
                     /locus_tag="ACGI6B_000820"
     CDS             complement(53079..53351)
                     /locus_tag="ACGI6B_000820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9750285.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(53381..54196)
                     /locus_tag="ACGI6B_000821"
     CDS             complement(53381..54196)
                     /locus_tag="ACGI6B_000821"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFG9750286.1"
                     /translation="MNIKSAFIRKRGEKFYVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPKDMTFVERCYKYMDMNKNNWSPYTVTNTKSWVKNYIEPLF
                     KNLKLTEVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVIREAYRLREIQENSCDFIK
                     LPSKEKYSTNEIYNRKEVLLLIEKLLSNNIEIPILLMLLLGLRMGEATGLRWSDVDLD
                     NNLINVNQILVYSNGKISFKEPKTPKSKRALSAPKELIEKLKIKKLRQSKLKL"
     gene            54412..55782
                     /locus_tag="ACGI6B_000822"
     CDS             54412..55782
                     /locus_tag="ACGI6B_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HFG9750287.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            56076..56234
                     /locus_tag="ACGI6B_000823"
     CDS             56076..56234
                     /locus_tag="ACGI6B_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750288.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   56520..57078
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56520..56549
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaatt"
     regulatory      complement(57219..57316)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            57642..57926
                     /locus_tag="ACGI6B_000824"
     CDS             57642..57926
                     /locus_tag="ACGI6B_000824"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9750289.1"
                     /translation="MEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHYRVLIKEKE
                     YLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED"
     gene            complement(58104..59099)
                     /locus_tag="ACGI6B_000825"
     CDS             complement(58104..59099)
                     /locus_tag="ACGI6B_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFG9750290.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(59220..60848)
                     /gene="aspD"
                     /locus_tag="ACGI6B_000826"
     CDS             complement(59220..60848)
                     /gene="aspD"
                     /locus_tag="ACGI6B_000826"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HFG9750291.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        23484 a         6968 c        10258 g        20350 t
ORIGIN      
        1 ttcattgata aaatgataaa aatatatctg atttcataca aaaaagagtg tctcatgaac
       61 taaaaagttc attttgagac accaccttta ttattttata ttatttatct cttgtatata
      121 ttactatagc atcccttaaa aacttagcta atccaggttg ttctctatca taatatgatg
      181 taaaacgttc atcatataca tacatctgtg ctagagagga atgagcttct ttagagtatg
      241 tgttccaagt gtaacacaac catttatgat gtaattttgc aacttcctga gccagttcac
      301 tagaggcatc accagtttta aaagcttttt taagtttact aattatttcg atatttaggt
      361 tttgccaatt ttcataatct tgcttagaca tatttttaag ttttttattt gatgtatcta
      421 taatatcttt accatatttc tctctaattt cagttccata atttttttca ttttcatcaa
      481 tcatcttttc tttaaaacct tcaaattttt ctatatctgt catagtgtat tttccctcct
      541 tcagagctat agttttagtt acattttcta ttagtaaatc aatttgcttt cttttggcta
      601 gtaatttatt atggtgttct tttaacgcac ttaaactatc gaagtttggt gaatttataa
      661 tgttttttat attttctaag ttaattccta attctctata aaaaagtatt tgttgaagtt
      721 tattaacttc attttgacca tatatacgat aacctgaaga atttatttta agtggcttaa
      781 gaagacctat ttcatcataa tatctaagtg ttctagtact tataccagct agtttactta
      841 atttttgaac tgtatattcc ataagtagta cctcctataa attaaatata cacttttacg
      901 caacgtaaat gtcaatacat taaaatataa aaatttatat cttttaataa aaatataaaa
      961 aaatatcttt actggatatt ttacacacaa ctgtcaatac tcactagtga aaattaaaaa
     1021 ataaaattta tagactatct ttactaaaaa atagaaggtc tatagtctag gaggaactgt
     1081 atgaatttaa aaagaagcaa tgaaaaacga gaagagtaca gaagaatgta ttcagataaa
     1141 gaaagttttt taagtttgat tcaaaatttt acaagtgtct caatagcaaa agaattaact
     1201 cttaaaaata tagagttaga aacatctttt atatgtgaat ataaaggaaa agaagttgat
     1261 ataatttata aggtattttc taaaagcggc aaagtatcac attatatagt gttagaattt
     1321 caaacagaaa tggatactga aattgtccca agattgaaat cgtatagaga acaaatttgg
     1381 aaaagtttta ttatgaaaaa aagccttgaa gaaatagaag ataaaaattt taaacttcct
     1441 aaagttatac cagttgtttt atatagtgga cctgaaaggt taagtagaaa aaaaggaatt
     1501 ttggatattg tagaatctat atcggagttc agtaataatt ctagtatgaa aaattaaaat
     1561 tacataatgt gtttattggt ctaaaatgga aaactgtcgc ataaacttga atatgaaaat
     1621 atttatagat acaggttcta tgtggaggtg ttccatgaga aataacaagt ctaaggtatt
     1681 gctaggaata ctttgtctag caacaggaat ggctgtaatt ctgtcaatgt tgcttcctgg
     1741 ctggatttgg tcagcactta ccgcattaat acttattgga tgtggagcat tattattttt
     1801 ttgttaaaat agttttaact ataaagtgtg gggtgagtat tgatgaaagt agtaacaata
     1861 aaattaccaa aatggatttc aagtataatc ctaaaattta tgagaaagag taaaaatgat
     1921 taaattaaaa agcctactta cggaagtagg cttttaaacg tggttactaa gctctttcaa
     1981 ttttaccaga acgtaaacat ctagtacaaa cttttactct cttaggagct ccatctataa
     2041 tcgctcttac gcttcttaag ttagctgacc atgttctttt attatgttta tttgagtgtg
     2101 atacttggtt tccagaaacc ttacctttac cacatacgct acatactttt gccatctacg
     2161 cacacctcct taaaatgata actatttaaa gactaaaaac atatttatat tatcataaat
     2221 aacaaaaatt tgcaatagaa atatatattt gccttacaca tattgaagaa tttcttatta
     2281 tcatataaaa ttatatatat aattttatat aaagtaaata tatttggaaa ataattatta
     2341 tttttctttg aatatatata ctatagaaga aattttatcc aaaaactttt ctaggtttaa
     2401 attataccat atttatagta tgatatgatt ataattttat ataataaata agtaaaaata
     2461 gtaaaaaata aattaagaaa tgtttttaat aaaaataagc ttaagggggt caaccatgag
     2521 tgcaaaggta acgaatcaat atggaaatat agaaatagat aatcaagtaa tagctcaagt
     2581 cgtatataga gctgcaatgg aaagttatgg cttagtaggt ttagcatata agtctaaagg
     2641 aatagtagag ctattaaaag gcgagaacgc aactaaaggt gtaagagttg aggaagtaga
     2701 agaagatgcc atagcaatcg aactttatgt tataatacaa tatggtacaa atatatctac
     2761 tgttgccaat aatataatag atagagtaaa atatgtagtt gaaaaaatga ctggcgtaag
     2821 agttactaga attgatatta atgtacaagg aataagagtt aagtaatttc ataggaggaa
     2881 acaaatgatt caatatatag atggtaaaag gttaagggag atgtttattt caggtgcaaa
     2941 caatctccaa aacaataaag agttagttga caagttaaat gtattccctg taccagatgg
     3001 agatacagga actaatatgt cattaacaat atcttatgcc ttaaaagaat tagcaaaagt
     3061 tgaaaatgat aatatatcag atatcggaaa agcattatct aaaggttctt taatgggagc
     3121 tagaggaaac tcaggagtta tactttcaca aataatcaga ggtatagcta aatctataga
     3181 aggaaaaagt aaattatcaa cagaagattt agcaaaagct tttaaaaatg gttcagacac
     3241 agcatataaa gctgtaataa aaccaattga aggaactata cttactgtag ttagagaaag
     3301 tggagagttt gcaataaaaa ctgcgaaaaa agagaaagat gtagtaaagt ttttaagtat
     3361 gcttgtgaag gaatcaaatt cttcattaga aagaactcca gaccttctaa agaatctaaa
     3421 agaagctggt gtggttgact ctggtggaaa gggtcttgta cttatatatg aaggaatgtt
     3481 agcttctata aaaggcgata acatagaaat aaaaaatgtt gacctagata ctaatatttc
     3541 aacaagtatg gattttgcaa agagtactac tagcacagat gatataaaat attgctattg
     3601 tacagaattt attttagaaa gttctaaggt agaagatact aaaataagag acataatgat
     3661 ggcttatggt gatagcctag cagtagttgg agatgatgga gtcataaagg tacatgtaca
     3721 cactaatgac ccaggaaatg tacttcaaga agctctaaag tacggtcaat tattaactat
     3781 aaaaatagaa aatatgaagt tacaacatga aaatacatta ctggatgtag aagaaaagaa
     3841 agaaaatgat agtgaacctt tagaagaaga aaaagagttt gggtttatag ctacttctat
     3901 gggtgaaggt ttggcaaata tatttaaaga tttcggagtt gaccatataa tagaaggtgg
     3961 tcaaactatg aatccaagta ctgaagattt tatgaatgct ataaagcata taaatgctaa
     4021 aaatatattt attttcccaa ataacagcaa tataataatg gctgctaatc aggcaaaaga
     4081 attgagtgat aaaaatataa tagttatacc tacaaaaaat acgcctcaag gatttgctgc
     4141 attagtaact tttaatggag aattaagcga agatgaaaat aaagaagcta tgatgaatgc
     4201 tttaaactca gttaaatcag gtcaagtaac ttttgctgtt agggatacag tgatgaatga
     4261 aattgatgtt aaagaaggta atataattgg aattgcagaa ggaaatttat tatcagcagg
     4321 agattatgtg gatgaagtaa catcaaacct tattgaaaag ttggttgatg aagatactgc
     4381 tataataaca ttattctttg gtgaagatgt tacagaatct caagcaaatg aactacgtac
     4441 ttctttagaa gaaaagtttg aagatgtaga tgtagagtta tattatggag gacaacctct
     4501 ttattattac ttaatttcag ttgaataatg attataagaa cctgtatgac caagtagctt
     4561 ttataaaatt ggttgtacag gatttttgtg tagtaggtga tatatttgtt ggatttatat
     4621 aaagatgttc aatatgttaa gggaatagga cctaaaaaag cagataaatt aaataaactg
     4681 ggtatattta cactaaaaga cctattgtat tattttccaa gacaatttga agatagaaat
     4741 aatctaaaga aaatagcaca attagaagat ggtgagaaag ttacgataaa agcagtaata
     4801 tcaagtataa atacatttag cccaaaagaa ggtatgactt taacaaagat tgatgtaaaa
     4861 gatgagacag gctcggctaa attagtgttt tttaataaga gttatataaa aaatactttt
     4921 cgtccaggag attcaatatt agtttttggg aaggttaaga aaaaatttaa taacttagaa
     4981 cttacatcat gtgaattaga atatcttact aattcaccta agaatacatg tagatttatg
     5041 cctgtgtacc agcttacata cggtgttaca aataaagaaa ttatgagtat aattagaaca
     5101 gtacttgaag ataaagaatt aattatacaa gaatatatgc cacaaagaat tatagaaaaa
     5161 tataggctat gtagtataga ttttgcagtt agaaacatac attcaccaag tagtaaggaa
     5221 tcattaaaaa tagctttata tagaattgta tttgaagaat tacttatact acaactaggg
     5281 ttgtttgtat ttaaaagtgg cagaaataaa gaagatggaa taaagtttga aacaagtaaa
     5341 gacttaaaaa aaataataag tgctttacca tttaaactga ctaaagctca aaacagggca
     5401 ttagatgaaa ttatacaaga tatgaattta gagaaaataa tgaatagatt ggtacaaggt
     5461 gatgtaggga gtggtaaaac agtagtagct ctcttagcac ttgcaaattg tgttttaaat
     5521 ggttatcagg gcgctttgat ggcacctaca gagatattag cagggcaaca ttatatttca
     5581 cttactgagt ctctaaaaga ttttggaata aatgtaggtc tattaatagg tagtcttaca
     5641 aaaaaacaaa aggatacagt attggaacaa ataaagaata atgagattga tatattaata
     5701 ggtacacatg cactaattga agataaggtt gaatttaata atattggttt agttataaca
     5761 gatgaacaac atagatttgg tgttatgcaa aggagcaaac tttcattgaa aggagcaaat
     5821 cctgatatat tagtaatgac agcaacacca ataccaagaa ctttagcact tatactctat
     5881 ggagatttag atatttctat tatagatgaa cttcctccag gaagacagcc aatagaaaca
     5941 attgctattg aaaaaagtaa gcgagataga gcatataata atcttgtaag aagagaggtt
     6001 gaatcaggaa gacaagtgta cattgtatgt cccttggtag aagaaagtga agccatagaa
     6061 gccaaatcag cagttgaact tgttgaggag ttaagagcgg aatattttca cgacttaagg
     6121 cttgggttgc ttcatggtaa aatgaagtca agtgaaaaag atgaagtaat gagacttttt
     6181 aaagataaag aaatagatat tttagtttca acaacagtaa tagaagttgg tgtcaatgtg
     6241 ccaaatgcaa cattaatgat aatagaaaat gcagaaagat ttggacttgc acaattacat
     6301 cagcttagag ggcgagttgg aagaggtagt cataaatcgt attgtgtact aatttatgat
     6361 tctaaaacag atgtatgtag acaaagaatg gctataatgg aagagaccaa tgatggtttt
     6421 aagatttcag agaaggattt agaaataaga ggacctgggg aattttttgg gacaaggcag
     6481 catggtcttc cagaacttaa ggtagcaaac ttatttaagc atataaaaat actgaagtta
     6541 gcacagcaag aagctcgata tatattgggt gaagataata atctacaatt aaaagaaaac
     6601 atggcactca aaaaagaaat tatagataaa tttaaagata cattgaaaga aatttcatta
     6661 aattaatttt aaattgtgtt aaaatattac taatgtaata atgtgctgaa gggagattgt
     6721 catattgaga gtaatttcag gaaaagcgag aggattaaaa ctaaatactc caaaaaatga
     6781 agatgtaagg ccaactacag atagagtaaa agagtctcta tttaatatga taaattcata
     6841 tataatggaa agtgaagttc tggatttatt tgctgggact ggttctttag gaatagagtg
     6901 cttatctagg ggtgccaagt catgtacttt tgtagatatt agtaaggaaa gcatagatat
     6961 tgttaagtcc aatataaaaa aggcaagagt tgaaagtgaa agtattattt taaaccttga
     7021 ttttaagact gcaatagata aacttaaatt acaaaatagt aaatttgaca taatttttat
     7081 ggaccctccg tattataaaa atatgtttat agaagctatt gaaaaaattg ataattctaa
     7141 tctattgaac gaagatggaa taatagttgt agaacatgat acgaatgatt tatttccaga
     7201 taagatatgt aaattggaga aaactagaga taagaaatat ggaaatacta cattaacttt
     7261 ttataagatg gaggcataat atggaaaata aacctagaaa agctatattt gcaggaagtt
     7321 ttgacccaat tactaatgga catttagata ttatttgcag ggcttcgaag ttatttgacg
     7381 aattacaaat aggagtatta aataatccta ataaaaaggg tctatttagc tttgatgaaa
     7441 gagtaaaact tatagaaaaa agtacaagcc atttaaataa tataaaagtt gtgacttttg
     7501 atggtttatt aataaattac tgtcaggaaa atgggattgg agcattagtt agaggtgtga
     7561 gaagtggtgc tgatgttgat tatgaacttc agatggcaca tatgaataga gagcttaatc
     7621 cagatataga gactataatt ttaccaagtt gtacaaaata ttcatttata agttcatctt
     7681 taataaaaga ggttttgctc tttgatgctg atataaaaaa tttggttcca aagattgttt
     7741 tggaagaatt aaagaaaaaa actagtgggg gtaattgata tatgaagata gatttagaaa
     7801 tgattgaact atttgagcag ttagaggaga tatttagaag tgcttctaca atccctttct
     7861 cacataaatg cacagtggat aaagatgagg ttttagcaat aattaatgat ataagaactt
     7921 taataccaga agaagtaaca caggcagtat ggattaataa agaaagaaat aaaataataa
     7981 gtcaagctaa gcaagatgct acaaatatag ttgaacaagc gaaaaaagaa gctgagagaa
     8041 tacaacaaga atatcaagat aatatagagg aattaaagaa aaattctgaa gatgtagtaa
     8101 aggcatatgt agaatccagt gaacctgttg ttcaagcaga ccaaagagca aaagatatag
     8161 ttgatagagc tgagagaata gccaatgaga taagaattgg gtcaattgaa tatgcagagg
     8221 atgtgctttc tagtgttgag tataatttaa gagaaatatt agaagaagta aaaagagata
     8281 aagctgaact tagacctaaa aaataaattt taaataaata aaaatggagc tatcacacaa
     8341 aaaattccaa tttatgtgtg atagctctct tttaatatag caattttttg ttatataatt
     8401 gtgcttttta gattacttat atcttatttt gatattataa tgtagtttaa tcttttattt
     8461 gttgaaatca acttatttat ttgtataaat aatataggtt attttcaaat ttttttaata
     8521 attttaccac tgtcttcttt tagattagag ttagactgtg caaaattata aaataatact
     8581 aaatcatgtg caattatttg gtatggcact acatgtagaa aatatagata ataaaattaa
     8641 ggaggtagta tgaaggaaag ggctaaaaaa atattagatt ttattattaa aaataacaat
     8701 gtaacatcac aagaactaca agaaaagttt aatgtttcaa agagaactat ttattacgat
     8761 attctagcta taaatgaaca actaggaaaa agtggtaata taaaaaatgt aaaacacaaa
     8821 tttatatttg aaggtaattt atgtgatgca aggaaaataa tttcaactga agaagataaa
     8881 tttttagata gtgattacag aaaaactttt atattaaaca agatattact tggcgaaaaa
     8941 atatcaatag aaaaactaac aaatgaaatg ttactttcaa aaaacacagt agtacaaaca
     9001 ataactgatg taaaaaaata ccttcaaaca atggggttaa gattagagta taagggaaag
     9061 tacaaaatta taggtgatga atacgttatt agagagctgt ttctgataat tgttcaagaa
     9121 aatatattgg aaattaattc tataagtgag gaagtaagta gtttcgatac aaaaggacat
     9181 ataaaactta ctgattattc tttattaaat ctaactaagt ttgtggaatt tctaaataaa
     9241 aggataagag atgggaaaac attatactca tataaatatc taaatgaagc taaaaaaatt
     9301 agttacttta gtaattgtaa agaactttta tgtgaagaag ctaatgaaaa tgaacaagcc
     9361 tatatatgca cttacataag ttcacttccc agcttaaata gtgaagttaa ggaagatgtt
     9421 gttgaagaat atgtagataa acttatagat aagtttgaag taaatacagc aataaaatta
     9481 gaaagtaagc atgagtttaa gaaaaatatt ttaaggcacc tccattcttc ttacaacaga
     9541 atacgattta agtttcctat tagaaatcca atgttagatg agactaagta taaacatgag
     9601 tcactttata aaattataaa atcaattata gaaaatgaag aagagtttcc tgtatttgag
     9661 gggataagag aagaagaaat aggatttata gcagcgtatt ttggaggata tttaagaggg
     9721 tctagggata atggtctaag aagaaataaa gtcttactag tttgtcctaa tggtcttatg
     9781 gtatcaaaga gtttggaaat acaactatat aaatacatac caaccataga aatagtaggt
     9841 atagtatcca ttaagcaact aaaagaagta aatgtatact acgattacat aattacaact
     9901 atagatatac aaaatgtaaa caacgtaatt gttgtaaatc ctcttttaac atcatcagat
     9961 gttcaattac ttatgaacaa actaataagt gtaaaagaaa acgagaagta tttcaatctt
    10021 gaattaataa tacaagctat aagaaaaaat ggagttatca ataatgaaga agcactaaaa
    10081 gctgatttat taaacataat acacaagata gatgaaggag agatgtatca accaatgtta
    10141 aaggaattaa ttaatgcaga aagggtcaat ataataaaaa gtgttagaga ttggaaagaa
    10201 gcaataaaaa tagcctcaaa gccattgtta gaagataact ctatagaaga gttatatata
    10261 gaaaatatga taaagtcagt tgagaaatat ggaccatata ttgttttagc tgatagattt
    10321 gctctaccac atgcaagttc taaagaagga gtaaacaagc ttgctatgtc tttactaata
    10381 gtagaagatg aagttgattt attaggaaaa ccagtaaata tatttatggt attagctgct
    10441 gtagataaca ctactcacat tagagcatta gcatcactat cagagatgat gtacgaggaa
    10501 gaaaatgtaa aactaataat taatggtgat aaaagttcaa tactagaact tataaataag
    10561 caaaattagg aggaatgaaa aatgaagata ttagcagtat gtggttttgg tgtaggttca
    10621 tcaatggtat taaaaatgac aatacaaaag gctctaaagc agttaggtat agaatgtgaa
    10681 gtagaaaata cagatataaa ttcagctaga ggtgctgatt gtgactgtat attcacaagt
    10741 caagaattgt taaatgaatt aaaaggaact tgtaatgtag atgtatatcc agttaaaaag
    10801 tacatggatt tagcagaagt cacagaagtt gttcaaactt ttttaagtga taaaaatgat
    10861 aagaaatagt ataagtttta aaaagtaaat ttaagttaat cagaataaaa taaaaactta
    10921 ctaaattcaa aaatagtaag aaagagagga gggatttagt ggtagaaaac ttaaacagtt
    10981 gacctatcac taaaaaatat aatgcttgat tttatttcaa ataatatttt acgtaatcca
    11041 tctatgttaa ttggattaat agcagcattt ggtcttgtac tacagaagaa gaaagcatct
    11101 gatgttataa aggggtcact acttgcagct ttcggaatta taattatgga gactggaaca
    11161 ggtatgttag ttagttcaat agcaccaatt aatggagctt tccaaagtat aagtggtggt
    11221 gcagtaacaa aaggtttaag tgatgttact tttactgcaa gttatggtgg aattataggt
    11281 ttagcaatgt ttgcgggttt agctattcac ctaatgatag caagatttac tcctattaag
    11341 acaatctttt taacaggtca catgctttat tggttcccat tcatatttgt agcttcagga
    11401 gttgaagctg gattaaatgg tggagtatta atagcttttg cagcagtatt atcagcactc
    11461 tattggtcaa taatgccttg gttattaaga aaatatgtgt ttgatgttat aggagatgat
    11521 tcttttacat taggtcatcc atctggttgt ttagcactta tatcaggatt tgttgctaaa
    11581 ttagtaggaa ataaagaaaa atctacagaa gatataaaca tacctcaaag tttatcattt
    11641 tttagagaaa tatctataag tggtggaatg gcagtgtttt taatgtttat agttgttggg
    11701 atagtaatac caggtgcgtt taaagatagt aatgagttta ttggaacgta tgcaataaag
    11761 caaggtcttt cttttggagc aggtcttata ataatgcttc atggggtaag aatgctaata
    11821 aatcaaataa tgccagcatt tcaaggtata tctgaaaaag ttgtgccaaa tgcactacct
    11881 gcttttgact gtccaatatt atttaattat aaaccaaatg ctgtattaat gggattctta
    11941 gttgctatgc taacatctac agctatgatt ttaatatgta actactttaa tgtatttaat
    12001 ttgctactta taccacttgt tataacttca ttctttgagt gtggagcagc agcagtaata
    12061 ggagaatcac aaggtggtat acgtggagct ataataggta cagctatagc tgcaatattt
    12121 atggtagttt tagtaggaat atctgctgta atttactcag gaactattca aaattggata
    12181 ctaatatttg gaggtaatga cctttcatta tttggtataa tcagtaggtt tataggttca
    12241 ctatttggag gaatattata gatatgaaat ttaaatacat agatgagata agaagattaa
    12301 ttgatataat agaaaacgaa gaaaaagtca atatggaaaa agcagtacaa atctttgttg
    12361 aagcaataga aagtaaaaat gctatatttt catttggagc ttcacatgca ggaatattaa
    12421 cagaagaatt attctataga gcaggtggct gtgttcttat aaatccagta tttgcaaaat
    12481 ctctaatgtt agatacagcg cctataactc acacaagtaa gatggaaaga ttagaaggat
    12541 atggtacagc tctagctgat aaaactccaa ttaaaaaagg agatgtagtt atagttcatt
    12601 cagtatctgg tagaaatcca gtaagtatag agtttgctat gaaagctaag gaaaataagg
    12661 caatagtaat atgtataact aatttaagtt actcaaaaag tgtatcttca agacattctt
    12721 caggaaaaaa actttatgaa gtatgcgatt tagtgataga taatcatggt caaaagggag
    12781 atgcctgtgt agagatagat ggattaaacc aaaaagttag tccaacttct actgtaatag
    12841 caactactgt gatgaactct attatagctc aagctactca ggaattagtt aataaagggc
    12901 taaagaatcc tccgattttc tatagtgcaa atattgatgg tggagatgag ttaaataaga
    12961 agatttttga tgaatataaa agtgtaattc attatgaata ttaaggttaa aaaaatatgt
    13021 taatgaactt tgaagtttat caacaaagaa tattaataat atataataaa tgctatcatt
    13081 taaaagctgt aggtaaaaat ctacagcttt ttattattgt agattaaaaa gatgcgtaat
    13141 taaattttaa attggagaaa ataaattgga aggtatcaat tataaatatt agaaacgttg
    13201 tcatagaaaa aaactatatt aaaaaaaagt acatagaaaa aactgattag acaataaata
    13261 catgctttga taatatttta aatggagtga aaatgtgtag aattataatg aaaaaagaaa
    13321 ataaaaatga aaaaagaaaa taaaatggag ggaataaaag tgggtatgtt aaaaaaatta
    13381 tgttcactag ctatggtagc aattatgact acaacactta taacagggtg ttcaagtgga
    13441 aatggaaaag attcaaaaaa ggatggcgaa aaagaaaaac tagttctttg gatgccacca
    13501 gtagaaaata atatgaagga agtttgggac cctattttag ataaatttga ggaaaaaaat
    13561 aactgtgaag tagatttaca gataattcct tgggataatt attctgaaaa atttgcaaca
    13621 gctataagtg ctggcgaagg tccagatgta ggatatatgt atgcagaaat gtatccacag
    13681 tttatagaaa gtggagcagt agaagatttg actaactatg caactaaaga ggataaagag
    13741 caatctattt atataaaaac atctgaaatg atgggtggaa tgtatggtat gccattccaa
    13801 gcagcaaatc caggggtatt atactataac aaggatattt tagagaaact aggagaaaaa
    13861 cctcctaaaa catgggaaga ttttaaaaga atttgtaaaa aagcaacgca agatactgat
    13921 ggtgatggaa agatagacca atggggatta gctcagggat ggggagctaa aacttttggt
    13981 aacttaaact ggaactggta tccttattta tggcaagctg gtggagatat atttaatgat
    14041 gatttaaaat cggttaaatt taatgataag agtggtttag aagcagctaa gttcttaaaa
    14101 gaattacaag catatgtgcc tgaagattct ttatctaaag atagtaatga aatgatagaa
    14161 agtgtatttg gaccagggaa agcagcattt acaataatgt tatcttctgc tgcaacaagt
    14221 gtatttgatg aatcattccc ggatttaaat tggggatttg taactggact agaagataag
    14281 aaagcagcaa cttttggagc agtagaccat ttatctttaa tctcatctgc gaaaaataaa
    14341 gaacttgcat acaagttaat acaacatatg ttaagtgttg aatcaatgac agaatttcat
    14401 aaagcaatac caagagcacc aataacaaaa ggtgaaccat atcaaggtga tgaaagattc
    14461 aaagaaatgg tggaaaatga caaaaatgta tatagaccat tagtagtagg acctcatgga
    14521 gttgaaatat atgaatattt atggaaagaa cttcaaacta tgatttcagg agataaaact
    14581 ccaaaacaag cgttagatga tgctgcaaaa tattcaaatg atttattagc acaataaaag
    14641 tttaaagctg taatatgttg taaagtactt tacagattaa aattagcatt gtatttataa
    14701 atacaatgct aatatttata ttggagagga taaaatggaa aacttagtta gagagtttga
    14761 agaaaattca aaaagaaaaa aaacaaaatt aaatattaaa ccatatatgt atatattgcc
    14821 attgggaata attttagtgg cgttttatgt gataccaata ataatgtcaa tatactttag
    14881 ttttacaaag tataatatta ttagtccagc tacttttata ggtttagaaa attataaaaa
    14941 attatttaca gatgaaacat taaaagtatc tattataaat actattaaat ttactgttgt
    15001 tgtagtacct tgccaaacta tattatcatt gattttggct gtttggatta caggaaaagg
    15061 aaatagtaaa atagcttcat ttgctaaagg tgctatattt ataccagtat tatcatctat
    15121 ggttttgata ggtatggtat ggagggcttt attgaatgga gaaggttcta ttatatatca
    15181 ggtattaggt gcctttggaa tagaatcatc taaactttta ggagatagta agacagcatt
    15241 acctacactt atgtttatat ctatgtggaa aaatataggg tatttcatgg taatttatat
    15301 ttcagctata atgaatttac caaaacactg ctatgaagta gcgaaggttg atggggctac
    15361 aaaacttcaa gaatttataa aaataactgt acctttattg aaaccaacta caataatggt
    15421 tgtattttta gggtcgatat ggtcattaca agtatttgac ttaatttata ctgtgacagg
    15481 tggaggtcca ggtatatcta ctatgagtat agttatgcat gcatttaact taaactttaa
    15541 aaactttaat tctggatatg caatgactgt agcaaacgta ttatttttgt taatagcagt
    15601 agtttcgatt ttacaaaata agcttgtaaa acgagacaat tcagattttt agaaaggtag
    15661 gtataatatg aaaacagcaa gaagattatt aagtgatggt ttacttttat ttatagcgtg
    15721 tgcaagtttg gtacctttta tatacatgtt gataatatca ttgaagataa catataactc
    15781 atatagtttg gatatatctt tttcaacagt aacccttcaa aactatatag atatttttac
    15841 taaaaaagga tttgcacaat atttttttaa tacagcaata gtatcttttt caggtgtatt
    15901 attaaattta gtatttagta cattagcagg atattctttt gcgaagatgg attttaaagg
    15961 tagtgataag ttatttttat ttatgataat gactttaatt ataccttccc aagttacaat
    16021 gataccacta tatataataa tgaaacactt aggatggata aatagttatc ttgcacttat
    16081 tatgccaata ccaactgcat ttggcgtatt tataatgaga caagctattt taggagtacc
    16141 aaaagagtta ttagaatcag caaagataga tggttgttcg gattttagaa ttttaataca
    16201 aatagtgttg cctttaatta aaccagcatt aattacacta gcaatattta cttttatggg
    16261 agcatggaac gagtttatgt ggcctcttat agctacaact aaagatgcaa tgagaacatt
    16321 aacagttgga ttatctactc ttaagacatt ccagataact aattatggac agatgatggc
    16381 aggggctaca ataacatttt tacctccatt tattttctat ttgattttac agagtaaatt
    16441 tgttgaaggt gtgtctttgt ctggtataaa aggttaaata gtctaattta ataatatata
    16501 aagaaactta tataaatatg gaggaattta aactatggaa atttcatatg tactgaaaga
    16561 ttgcacattc aaacacaata aatactcagt tgtagaagct gaaacttgga aagataagga
    16621 tttggatatt gtagtagcta aaggtgaaaa atttgcattt caaattatgt taaaagcaac
    16681 agaagaattt aattgtacaa ttgataaaag tagtgctata tcatggaaag gcctgggaaa
    16741 tagagtaagg ttagcattaa atatgcctaa tagtttagaa aataatttta gtataaatat
    16801 attaggatat gttcaagatg atacaaaggc atttgtgaat gatgctattt taagggataa
    16861 agatgtgtta gtagagcaat atttgcctca aacattttgg attgaaggtc aagtaccaga
    16921 agattttgaa gaaaataacc ttgatatagg tattgatata tttaaaagct ttggatatga
    16981 agatgaagaa aaagtttgta caattgatgt tcctgtaaaa gtaagaaatg ttgtgctaaa
    17041 gcctttagat gaaagcaagt tctttttaga tttatggcaa catccaagct gtttagctag
    17101 aatgtataaa gttgagttat ggtctgatat acattttgaa atagtcgata actatttaaa
    17161 agaattagca tctttaggag aaaaagttgc aacagttata gtttcagatt atccatgggc
    17221 agggcaaagt tgttataaag tttataaaaa tccatcaaac ttatatgaat ataacatggt
    17281 aagtgtatca aaaggattgg atggaaagat taaatgtaac tttgagagta tggataggta
    17341 tataggcata gctgataagt ataaaatggc taaggagata gatttatttg gtctattagg
    17401 taattggtgt gctggtgagt ttggaaatcc tgtagaaggt tataaggacc ctataagagt
    17461 aagatatttt gatgaaaagg ataaagtatt taaatttata aacaatacaa atgatttaaa
    17521 agaatatata ggattagtat taaatcattt aatagagtgt ggattatggg acagagtaag
    17581 gattatagct gatgaaccaa ataatccaga agtagtcaaa gagtgtatag aatttataaa
    17641 ttctactgta ggtactcatc aagttaaata taaatcagca actcacgacc aaaacttttt
    17701 agacagagct aaagatgaaa ttgatgatat gtcaataaat ttaaaactta ctatacaaaa
    17761 ttataaagat atagaaagtc ttaagaaaaa aatcaatgac aagggtggga tattgacttg
    17821 gtttgtatgc tgttttccag aaaaaccaaa tagtttttta agttctcctt ttgtagaaaa
    17881 tagaataata ggatggtata cttattactt tggtcttgat ggatttttaa gatgggacta
    17941 taatttatgg acagaagacc cttggaaaga ttcaagttac aaattcccta tttggaaagc
    18001 tggagatatg ttctttgttt atcctggaaa agatttaaag ccagttagaa gtgtcagaat
    18061 ggagaattta agatttggaa tacaggattt tgagttattt acaatgttag aaaaagaaaa
    18121 ggtaagagag tatatagaag ttgagcttat gcaagaatta ttaaataaaa aagaaaatgc
    18181 tgaaattaaa ggttttggtg acatagaact aggatattca cttgataatt ggagatatga
    18241 taaagttaaa aaatatgtat tagagttgtt agacagagta taaaaaatat aagagtgtct
    18301 caaaatgaac tttttagttc atgaggcact ctttttgtat gaaatcaata tagtttcatt
    18361 gtttcaacaa tgaaaaatag gattatttct attctaagac agccctttag ttttttagtt
    18421 gtattcaatg ttttaaaact aactataaac gctataaagt ttataaatga caataattat
    18481 atataacatg atgtacttta atttgctata atagggattg aataatatta tatggaagat
    18541 aacattgttt ggggggatta atataaaaag aataggtatt attataatgt tttttttaag
    18601 tatatctttt attggatttt atcttgtaat aagagataac agtgatacag ttaagatgca
    18661 acgagggata aatataggta atgcactaga atcacctaag gattttccgt gggatgtaaa
    18721 aatgtcaaat aagttttttg atgatataaa agatgcaggt tttgacacag tacgtatacc
    18781 agtaaggttt tctgattata cgtcagattc tgataatttt aaaattgatg aagaattttt
    18841 taaaaagatt gataaatatg tagattatgc attggataaa gacttaatag ttgtgttgga
    18901 tttacaccat tttgaagaaa ttatgaaaga accaagagtt cataaagaaa agtttttaaa
    18961 aatatggcaa cagatagcta ataggtatca aaaatatgat aaaaaattgg tatttgaatt
    19021 attaaatgaa cctaaagaaa atttatcttc acaattacta aacgaataca ttgaagaagc
    19081 aataaagata attagaaaaa ctaaccccaa aagaactatt atagtaggac catataattt
    19141 ctatcaaatt gattatttga atgaattaaa tattcctaaa gattctaata tagtagtttc
    19201 ttttcactat tacgagccaa atgattttgc atttcaagga aatatctatc ataagggatt
    19261 tgagcactta agcaatatta cttgggaagg aacaaatgaa caaatggatt atttaaaaaa
    19321 aaggtttgat actgtggaaa attgggcaaa taaaaataag gtaaaaatct tcttgggtga
    19381 atttggaata acaaaagagg caccagaagt ttcaagaaga gcatggataa aagctgtaag
    19441 agaagaagct gaaaaacgaa acttttcatg ggcttattgg gagcttgcat ctggatttgg
    19501 tatatacaat caaattgaag ggacttggga tagagatata ttaagtgcat taatagaaaa
    19561 gaggtgattt tatgaagaaa aattcaaaaa agatacttat aactttaact attattacta
    19621 atgtcatata tatattgtgg agaatttttt acacagtacc taaagaagaa gggaagtttg
    19681 ccttaatatg tgccataata cttctttttg tagagataat gggtatgatg gaaatgtttg
    19741 tgcattatta tggtatgtca aatattgagt atcctgaaaa accaatcata agtgaagaac
    19801 tttatccaca tgtagatgta tttattgcaa catataatga atctgttgat ttggttagaa
    19861 aaactgtaaa tggatgtatt catatgcagt atccagataa gaaaaaggta catatataca
    19921 tatgtgatga tggaaatcgt gaagaaatgc gtattctagc agaaaaaatg ggtgtaaatt
    19981 acataactag aactgaaaga gaaggagcaa aagctggaaa tttaaataat gcaatgcaac
    20041 atacaacttc tcctttgatt gctacatttg atgcagatat gataccaatg catgactttt
    20101 taattgctac agtaccatac tttttaaaaa atgagcaagc taaaaaagat ggagaaaaag
    20161 aggagtatga aaaggttggt tttgtgcaaa ctccacagag tttttataat ccagatttgt
    20221 tccaatttaa tctgcactct gaaggaagga ttccaaatga gcaagattat ttttacagag
    20281 atattcaatt ggctagaaat aggacaaatt ctgtaattta tggtggtagt aatacagtta
    20341 tttcaaggga agcacttgaa gaagttgatg gattttatac ttattctatt acagaagatt
    20401 ttgcaacagg tatcttaatt caaagtaagg ggtatagatg ttatgctatt ccagaagtac
    20461 atgcatcagg tttatctcct acagatttaa aaagtcttat taagcaacgt gaaagatggg
    20521 cacgtggatg tatacaaact ggtagacgtc taaatatttt gtttaggaga ggtttaggtt
    20581 tttggcagaa aataagttat atttcttcaa ttacttattg gtatgcaagt ataaaacgct
    20641 ttgtatatat aatggcacct attttatttt ctgtgtttaa tgtgattgta gtaaaatgta
    20701 cgttacttca agtattggta ttttggttgc caatgtatat tttaagtagt ttatctctta
    20761 aaatattttc tcaaaatata agaaatacta gatggaccaa tatatatgaa actataatgt
    20821 ttcagtcttt aatgccagca gtaatacttg aaacgtttgc aatatctaaa aataaatttt
    20881 cagtaacaaa taagagtaaa ttagaagaaa atagaatgta taagttttta cagggaatac
    20941 cttattttat ttacatggtc ttatctatca taggaatttt aaaaatgttt gtagcaatat
    21001 ttaaaatgag ttcaatgaca tattcagttg tattattttg gttgattgga aaccttttta
    21061 accttgttat ggctacatta tttatatcag ggagacagca gttaagaaag tctgagcgtt
    21121 atattgctga aattgatttt aagttaaagc aaaattctta tgttctttct tcaaaaacaa
    21181 ttgatatatc agaaaatggg tttgcatttt tgctagaaaa tccagaatat atttcaccag
    21241 aagaagagtt tgaggtagag tttagagaaa aatctggaaa tgaaatgtat atagccaata
    21301 tgaaagcaaa gattgttaat gttgtggaag taaattcaaa gtggaaatat gcagcttata
    21361 ttacacatat tgaagatagt gaaatagata attggatgtg tattgttcac gatagaatac
    21421 ctactttacc aatgacaata tctaatcaac taggtttctt tgatgatttg caaattaatg
    21481 tgaaaaaacg tattgaaaaa actagaacat tatcacgaag aagtccaaga atcaatatga
    21541 attttcagat ggatataaaa aatacaggta aattaagaat agtgaatttt aattatcaat
    21601 atgtattatt aaattttgag aataaaagta tttatcctaa agaaatagca ttggaaatta
    21661 atgaaaatat tgtgttagaa tgtgatttgt gtgagggtaa aattgatgaa agaggtatct
    21721 tatataaagt aaataacatt gacagcataa tgcaaaattt ctttttaaga gatgaaatga
    21781 tggattggat attacaaaat aagacaattc ttgtttcaaa gcctagtgaa aagaaggaaa
    21841 aaagtataga tgagtttgaa cccatggaat atatttagga gagatgaaat gaagacttta
    21901 aaagtgtttt tttgttctac aaaatatctt attttggtgt ttattttttt tataaatact
    21961 acttttataa gttcagcaac aagtgataaa attttagatt tgtcctttaa aaagatagag
    22021 actgacttaa attctaagat tacatatgaa gatacaggtg taaaaataga aacggatagt
    22081 tctaaaagcg ataaggaacg ttatttatat atttaccaaa atataaaaga aaattggagc
    22141 atgtataata atttttatat tgaaattcaa aataaaaata aaagttctca gagaataaat
    22201 ttaagcatac aaagtaaaaa tatgcttgaa tttagattaa aagaaggtag tgaagtattt
    22261 ttagaaggta aaaatataat atattcagat aaaataaaag aaggatgcat agaagtacca
    22321 ggtgaatttg aaggcaaaat ttatgtgaac tttaatagcc ttataaatga ggaaagtaat
    22381 gttgtgctgg actcaaatat gctttcaaac attgttagtt ggggaattac gtttattcca
    22441 tctgatgaag aacataatat tgttattata aaaaagatat ctttattgtc tgaagagaaa
    22501 ttaagatttt taaataatat taaaataatt ggcgatgaag aagtacaaat accagtattg
    22561 ggtcaaagta taagtcagta tgaagtatta ggtcttaaaa gtgattctaa aattaagtac
    22621 tctcttatgg gaaaacaaga taatgtaagc atatctcaaa aaggtaaatt aacattaagt
    22681 aataaagcca aacctggaca aattatttta caagttaatg ttgatgataa gtttaaaatt
    22741 ggaaagaaaa taacattaac tgagtcttgg agtgttaata aaaaagataa ggatggtgtt
    22801 ccatatacat tagtatatcc tgagcaatca ccaacagtac aagatatgaa aaaaataaac
    22861 tttatgaata acatcataac gtttgttcga attttatttg tatctctagt aataatatgc
    22921 tttggaatat atttatattg gaaaaaatgt tcaaaaacta agtaaagaga ggaggttaga
    22981 ttgtgttatt ttcaagttta atttttttat tttatttttt acctataact ttggttttat
    23041 attatgtatt taggtttaac cgaacgatac agaacatgat attgcttgct gttagtttat
    23101 ttttttatgc ttggggagag ccaaaatttg ttgttatcat gatagtttcc ataataatga
    23161 actatatatt tggtttactg gtagatagat atagggaaag taaaataaaa gtaaaagttt
    23221 ttttagtttt gatgtgtgca tacaatatag gtgttttatt tatatttaaa tatctagctt
    23281 ttgcgctaag aaatattagt gaacttatta gtactgaatt gactatacct agtattgttt
    23341 tacccatagg aatttcattt tttacatttc agggaatgtc ttatgttata gatgtatatc
    23401 gtagacatgg agaagtccaa aagaaccctt tttatgtggg attgtatata gcatttttcc
    23461 cacaattaat agcaggtcct atagttagat atgagagtgt ggcagaacaa attctcaata
    23521 gaaaagaaac ttggaataaa ttttctattg ggacttgtag gtttataaca ggtctaggta
    23581 aaaaagtatt aatttcaaat aacatggcta tagtagcaga ctatatttac actatgaata
    23641 gtcaaggaga aatagctgca tcacttgcat ggcttggttc aatagcctat actctgcaaa
    23701 tattctttga tttttctgct tattcagata tggctatagg cttaggtctt atgtttggtt
    23761 ttaaatttga agagaacttt aattatccat atatatcaaa atcaattagt gagttttgga
    23821 gaagatggca catttctttg ggaatgtggt ttaagagcta tatatacttt ccgcttggag
    23881 gttctagagt agcaaataaa gatattatga ttagaaatat gtttatagtg tggttattta
    23941 ctggaatatg gcatggagca gaatggacat ttgtaatttg ggggatttta aactttgttt
    24001 tcctaattat agagcgattt ataatgtttg aaaagattga aaatcacaat tttataaaac
    24061 atatttatgc tttattagta gttaattttg ggtgggtatt atttaggtca cctaatttaa
    24121 aagaagcata taattatttt aaagctatgt ttggaggcaa tggagctata tggagtgatt
    24181 atacatatat gtttttaaaa gaatactggg ttttctttat atttgcattt atttttagtg
    24241 tacctattgc aaagaaaatc aataagtttg ttgttgaaca agctaaatat agtatgatat
    24301 ttaacgtatt ttatccaatc tcagctattt tattgttttt tatttctgtg acatatttag
    24361 taactggaag ctataatcca tttatttatt ttaattttta gttgggagga aaagtatgaa
    24421 tagatttttt aaagtaagat taataactgc aattatgttc ttaattattt tattcgcttt
    24481 ctctatcttt aatgtttgtt attctggaaa agatacgata caggaaataa aaaattcaat
    24541 agctgaaaaa agtagtttaa aagatacaat atcatctatt gataatacag tgaatgacaa
    24601 agtaatatac aaaaatacgt tcattgaaac ttatggctat ttacaaaaat taatgggtaa
    24661 aaatgagttt tcaaaattta gtgttgtaaa agatacttct ggaaagatgc attatacata
    24721 ttttgctaaa gctccaaatc cagttggtgt gatagcagat agggtaagaa aattttctga
    24781 tgaaatggaa aagcaaaaca ctaaattggt ttacctaatg actccagata agtatataaa
    24841 aggagtgaca aaatttccaa aaggaatacc ttattcttat agtaatgaga cagcagataa
    24901 ttttttaaat caactaaaaa agtataatgt ggactatatt gattttagag aaaatatttt
    24961 aaagagtggt attccaaaag atgatttatt ttttaaaaca gaccatcact ggaaagtaga
    25021 aacttctttt tgggcatttg gagaattagt tgagcaactt aataaaaaat acaatatgaa
    25081 tttagatgaa aatcattatt atagagataa ggacaattat aattcaattg tatatcctaa
    25141 gtcatttctt ggttctatgg gaagaaagac tggtatctta tatggtggta tagatgattt
    25201 tacattaatt tatcctaaat ttaagactaa ttatacttat tatacagatt ctaaatctca
    25261 aaaatttgaa ttaaaaggaa ggtttgaaga gagtttaata ttatcatatc cttttaatgc
    25321 agaccttgat ttaatggatg gtcaatcaga taagtatttt acatatttat tggggaacag
    25381 accattagtt aaaataaaaa atatagaaaa tccagatgga ttaaaagtgc tgtttgttaa
    25441 agattctcta attgtaccaa cagcagcatt tttttcctct gtttgttcat ctatagatat
    25501 gatagaccct agatattatg atggagatat actagaatat gcaaaatccc atgactatga
    25561 ctttgttttt atatctgtat atccccaaaa tcttacaaaa gaattttttc cgttttgtga
    25621 gtaaatttct aaaaaagttt tctgataaaa taaatggcta actggtaaaa agttagccat
    25681 ttattttgag tgttattcta agaaatattt tttttactaa atacttcttt atctattttt
    25741 ccaagtgatt ttcctacagt aattgatgat accatagcac cattaacatt tatcatagta
    25801 cgtcccatat ctagtatagg gtctattgca agtataccac caactaatgg aaaataagaa
    25861 ccaagaccaa ctccagagat aacaactgaa acggccattg tagcagttcc tggaagacca
    25921 gctattccaa acgaactaat tgttataaca attaacaaca taacataaaa gctcatatcc
    25981 atgtttactc cagccatatt ggcaagtgtt acagccatta aagcaggata tatacctgca
    26041 caaccattca tacccatatt tgcacctaaa cttgctgtaa agcttgatac accctcatca
    26101 actccgtgat tttgtactag actttctatc gttacaggca atgttcctaa actagaacgt
    26161 gaagtaaatg ccagaagtaa agcttcagtt gaatttttta tataagtaat gggatttaga
    26221 ccatttaaag ttataaaaat taaatgtata ataaataata ttgcaactga tatataaaga
    26281 gctaatataa actgtaaaac agatactatt gaactaatac cttgtgaagt tattgtattt
    26341 gcaagcagag caaccacagc ataaggcata aatttaataa cagtcataga cacacttaca
    26401 attattttgt aagatgcttc tataaaacct ataaatggtt ttattgtttc aaaatgtttt
    26461 ttacttaatc tctttattga ggtagctata aatgtagcaa atattattgt agctacaata
    26521 tttccatttg ccatagccat tacaggatta gatggtagta atcctcttaa agtatccaca
    26581 agagagttta tttctttaag ttcttgattt tcaactaaag taggcatatt agctcctaca
    26641 cctaacttca ttatatttgc aacgataatt ccaacaattg cagctatagc agtagtagca
    26701 agaagcatac ctaaagattt aaatgtcaga gttccaagat tgttatcttt catatttata
    26761 attactttta taatggataa gaaaactaaa ggtactacta acatttttaa taagtccata
    26821 aatccagaac caaataggct ataccactta cttacttctt gtagccaaat gacttgagtt
    26881 gtatcattag gaaaaccagc aaaagcttgt attatgattc ccaatatgag tcctagtact
    26941 gtagcatata ttgttctcaa tgaaaatttt atttttttct tttcaaattg tctaacaata
    27001 aagaacgtac ctagaagtaa tattatgaaa aaaattgttt taaaatctgt aatcataaga
    27061 aattgtgata aaaatgaact ttccaatatt tttctccttt tatttaaaaa taaatttttc
    27121 taagtttaaa tgatatcaat atattaaaaa aatgtcaata ttatatcaca atttacttaa
    27181 atagagtaaa acaagttgat tttgaaaaat gtgattaagt acagaaaaat agggtataat
    27241 aaatgtttaa agaatttgaa ttgtggtaag aaaataaaaa atagctttta taagatttga
    27301 tttcaaatta aaatgtgtta aataaataac taaaaattat tataaaaaat gttttcatag
    27361 aatatatatc aaatgaaaag cttaaaataa gtgaagaaat taactagaca tcaaaggaga
    27421 gataaaatga gagtaataat aattggtggt gttgcagctg gtatgagtgc tgcagcaaaa
    27481 ttaaaaagaa taaaacctga gtatgaagtt gttgtttatg aaaaaactga aatagtatct
    27541 tttggtgctt gtggattacc atactttgtt ggtggatttt ttaatgatgc tgatgaatta
    27601 ttagcaagaa caccagagaa acttagagaa gctggaattg atttaaatat atttagagaa
    27661 gtcgttgagg ttgatagtga atctaagaaa ataaaagtaa aaaacataaa aacagatgaa
    27721 atatatgaag attactatga taaattgatg atagcaactg gagcaagaag tattatgcct
    27781 cctataaaaa acataaaatt aaagaatgtt tctacattaa aaagtttata tgatggagaa
    27841 tatttaaaaa aacttttaag taatgaagat aataaaagag tgacaataat aggagctggt
    27901 tttatagggt tagaagcagt tgaggcatgt aagaaattag gtaaagatgt agatgtaatt
    27961 cagttagaag atagaatatt gccacaagta tttgataagg aaatgacgga tgtattagaa
    28021 gaagaaataa atagacataa tgttaatttg cacttagatg aaatggttgt agaactttgt
    28081 ggagaagata aagtagaaaa agttataaca aacaaagggg aaatagatac agatgtagta
    28141 ataattgcta ctggcgtgag acctaatacc gagtttttgt caaatactaa tataaaaatg
    28201 ctaaaaaatg gagctatagt agttgatgag tatggaagaa cttctgtaga agatatatac
    28261 tctgctggag attgtgcaac tataaagaat atagtaagta atgaaaatgt atatgttcct
    28321 ttagctactg gagctaataa attgggtagg atagtaggag aaaacctagc aggaagagaa
    28381 atatcttatc aaggttcact atcttcaagc tgtataaaaa ttatggatat ggaggcagca
    28441 tcaactggaa ttacagaaag gcaagctaaa gatttaggca taaatgtcaa atctaaattt
    28501 atttctgatt ataatcaaac tcattattat ccaggaagaa ataaaatata tgtaaagctt
    28561 atttatgatg cagatacaaa agttatttta ggcggacaag tggctgggtt taaggatgct
    28621 gtccaaagag caaatgtatt ggcagctgct atctttggaa aaatgaccac aaatcaactt
    28681 ggaatgttag atttatgtta tgctccacca tttgcaagaa catgggatgt tttaaatgta
    28741 gctggaaatg tatctaaata atatttagat tatagctaat atagttgaca agcaattaag
    28801 ggaatgaatt tacctaaact atttttatca tggtaaaatt cattcctaaa tttttattcc
    28861 aaatttttat tccaaatttt tagaaataag gattccatta aattattcat caaaatttaa
    28921 acgcaagctt aatctatctt ctccaagaat agtattttca aaaacattag tagcattttc
    28981 taaatacata ctaggtatgt ctaaagaagg actaaaatga gtcagtaaaa gttgtttgac
    29041 atttccaagt tttgcaagat tagcagcttc tctaaaagtc atatgtttgt ttctaacagc
    29101 ttttgatata tctaaatcat caccatacat ggcttcacat ataaataaat cactatcttt
    29161 tatgaaatct ggtattgatt ctataggtct tgtatcggtt gtaagactta tcttaactcc
    29221 ctttctcaat tctcctaaga ccatatctgg agtgtattgt tttgaatcta aaactatatt
    29281 ttgaccttct tggagtttgt tccataatat tttaggaact tcgtttttag ttgccttatc
    29341 tatatcaaat ttagggtttc ttctaaaatt aaaactgtat cctaaacact ctatagagtg
    29401 ttctaaactg attgttgaaa tttctaaatt tcttaaaact ttattatcta aagaaaaatt
    29461 gccttgaggg ttttctataa tttttaaagt gtatggtaca tattctacta aatttctcat
    29521 agaacgtata caatcgacta ttccaactgg accaactata gttaaatcac ttgtacgacc
    29581 actattccca attgttgata aaagtcctaa aagtccaaat atatggtcac catgtaaatg
    29641 ggttatacaa atcaagtcta tatctttaaa gccacatttt tttagcttca tagatacctg
    29701 agtaccttca ccacagtcaa ttagtatttt tctaccttta tagtttataa atacagagga
    29761 tagaaatctg tttggcatag gtacatttcc accacatcca agtaaagtta aatctatcat
    29821 tttttcccct ttctaaaatt aacacaaatc tgttgtttgt ttttattatg atatcctatt
    29881 ataattattt taacattaaa tataacaata aatgtgtatt tgagtcaaga aatattgtca
    29941 aataaagttt ttatatagca atgtataaaa atttggaaaa cgaaaagtga aaattatatt
    30001 taggagtatt taaaggagga aaatttaatg aaagtattaa agaaaattac tattttgtca
    30061 ttaattacat taaccttagt tggttgtagt aacggagaag agaaaaaagt ggttgaaggt
    30121 tctaagaaaa ctcaaaattc taaaaaagat gaacagccta taatagtaca taaagatgca
    30181 aatatattga aagataggta tgagccacca aaaggattta aaagagttga tgtagaaaaa
    30241 ggtagttttg aatcatttct taggaatact aagctaaaaa attatggaga gaaagtaaag
    30301 tattatgatg gaagaactaa ggaatcaaaa ggtatttatg atagtgtatt tgatgttgat
    30361 attggaaata gagatttaca tcaatgtgca gatgcaatta tgcttatgag agcagaatac
    30421 ttgtatcaga ataaactata tgatgaaata agttttgatt ttgttgatgg ttttaaggcg
    30481 aagtattcaa aatgggcaca aggatataga atttcagtaa aagatagtgg ttcaagctgg
    30541 tataaagcaa ctgaatattc aacatcttat gagagtttca gaaagtttat ggatatagtg
    30601 tttgcatatt caggaacgtt atctcttgaa aaagaattgg agcctgtaaa agttgaggat
    30661 atgaaaatag gagatgtgtt tatagttggt ggaagtccag gacatgcaag tataattatg
    30721 gatatggcag aaaatgaaaa gactggtgaa aaggtattta tgatagccca aagctatatg
    30781 cctgcacaac aaacgcaact tcttattaat cgtaaaaatc caaatttgag tccttggtat
    30841 agtttagatt ttgaaggtga tttgacaata cctcaatgga cttttaaaag agaccagttg
    30901 aaaagatttt aaaataataa ttttatataa taaaagaaaa atatagatgt gaaaaaatgt
    30961 aattagatta aaactaattg gatatggaca aaattaaatt gatagataaa tattaaaaat
    31021 tacactaatt aatcttacta tagtttaata tttatctaaa aagttttatg taaaatataa
    31081 attttatatt aaagctaaaa ctaatttgag gaggctaatt tatattgaag attagaaggt
    31141 atttaatttt ggtaattata attctaatta caacaagtca gagttttatt tcttatgggc
    31201 tagagcgatt tgaagaaata accatttttc acactaatga tatacatgga agatatgctg
    31261 aaggtgatga ccatatacaa ataggaaatc tagccacatt gaaaaaagaa actccaaact
    31321 caattttagt agatgcagga gattgtttgc atggacttcc aatagtaaat atggatagag
    31381 ggaaaagtgc aattgaatta attaaatgtg ctggatatga ctatattact cctggaaatc
    31441 atgactttaa ttatggaaaa gatagactgt ttgagttatc taaaagtgct aatttaggag
    31501 agaatacttt aaaattttta tcatcaaata tttttgaaaa tgggaaaaaa gtttttgata
    31561 gtaatgatat aaaagaaata gatggaataa aaataggatt ttttggactc tcaacccagg
    31621 aaacaaagag caaaactgga tttaaaaatg tggaaggatt agagtttaaa aatcctatag
    31681 aaagtgcaac agaacaagta aatgaattaa aaggaaaggg tgccaatgta atagttgcaa
    31741 tatgtcatat tggtacaaat agtttaagta agccaaatag tatagatata gcaaatgagg
    31801 ttcaaggaat tgatttgatt attgatggac acagccacac taagtttgaa aatggtaaac
    31861 aagtgggtga tacattgatt gtaagtgcag gacaatattt agaaaatatt ggcgagataa
    31921 aactaacttt ggatactaat gatgtagaag atataaaaat agaaaataaa tcagctagat
    31981 taattggaaa agaagaagca ttaaagtata agcttgactc cgttgtaagt aaaaaggttg
    32041 ataaaataaa agaacaacaa gaaaaaatat tagataaagt tataggggta actacaaata
    32101 ctctagatgg ttcatatgaa aatgtaagga caaaagaaac taatttgggt aatttaataa
    32161 gtgacatttt actagataaa actaaggctg atatttcttt atttaatgga gggaatattc
    32221 gagatacgat agaaaaaggc aatataacaa gaagagatat tgtagatgta tttcctttta
    32281 gcaatactat agttaccaaa gaacttacag gagcacagat taaagatgta ttggagcatg
    32341 gagtaaaact ttatccagaa aaaagttcag cctttttgca agtgggagga atttcatatt
    32401 actttaatcc taaacaaaaa gaaggcgaaa aaataacaaa tattcaaaaa gaaggaaagc
    32461 cattagattt aaataaaaag tatgtagttg caacgaatga ttatatagca tcaggtggag
    32521 atgaatttcc ttgtttttca aaagagccag tactaaagga atttggaaat ttagagagtg
    32581 cagtcattga atatatagag tacaaaaaag aaatatcaaa aaatgtagat ggaagaattg
    32641 gtattaaatc taaagatgag ttaataagca agaatcatga gagtgatttg atattaagtg
    32701 aaaatgaaaa aaatatcaat gataatgaag agaatctatc taataatata aaaagctctg
    32761 atagtgataa aaataaaagc ttagataaaa caagttctag agaaagttct aatctaggaa
    32821 tatctaaaag agaagctaaa gaaaaatctc ctaaaacagg agatttaggt ttttcaaata
    32881 gtataattat atttatagtt tcaagtacat tgatatgttt attgaatttt aatcaaaaag
    32941 agttaaagga taaaaagtca aaataaaaat taatgagcaa aaattagtag atagtaattt
    33001 aattttagat aatagtaaac agcaaaggaa attagttata taaatactaa tttcctttgt
    33061 tttatgtttt aacatgatta cttttaaata aaattgtatt ttatgaaaaa taattatact
    33121 aaatggcata gtattatggg gatttttagt ttttattcaa gtaaatgaaa tcatattaac
    33181 aacttctgat atatttacaa attgataaaa aaatgatttt attagtatga tataatttag
    33241 ctagtatgta tgataatgga ggatataggg atgaataata tattactttt ggaagatgac
    33301 aaatctttaa atagaggaat tagttttaaa ttaaaaaaag aaggatacaa tgtttttagt
    33361 gcatttggta tagaagaagc aaaaaatatt ttcgcaaaag aagagatatg cttaattatt
    33421 tccgatatag gtctacctga tggtagtggt tttgactttt gtgaggagat tagaaagaaa
    33481 agtgatgttt acataataat gttgacagct cttgatgaag aggtggatat tgtcacagga
    33541 tatgatttag gtgcagatga ttatatcaca aagcctttta gcttgatggt attaatatca
    33601 aaagtgaatg cgcttatgaa aagagtaaat acagtaaaaa attatactct attagtttgt
    33661 gatgatttat tcttttatta tatagaaaat aaattaatta taagaacaga tgataaggaa
    33721 gaagagataa ttttaagtaa gacagagaca aaactattaa aatatttgat ggaaaattct
    33781 atgcagacat tgactaaaga gcaactgtta gaatctttat gggatagtag tggtaatttt
    33841 gtagatgata atacgattgc agtaaatata agaagactta gacaaaaggt tgaaaaaaat
    33901 ccatctgaac ctaaatatat aaaaacagta agaggggttg gatatatgtg gggagaaagg
    33961 agtataaaaa aatgctaaaa aatatgaacc tcaaagatgg tttaagtgtg aaagatgatt
    34021 atataatgag aagagttatt gtatattctg ttttatctgt actaatagta acccttgtat
    34081 ttatactttt tgaatatagc agattacata aattgtatga acatcaaata aaaaatactc
    34141 aaaatgttgc tggaatattg attgaaaaat atcctaatga tgaaatagat atagtaaatt
    34201 ctatttataa ttctgattat agtaaggtgt ctactggaga aaaggcattt aagaaatttg
    34261 gctatggatt ggaaaataaa atgtcaaatg ataaaaattt tagcttatac ttaaaatctt
    34321 tttttaagga atcatttttt atttttttaa tgatgtttat agtagttata ggtataatat
    34381 attattttat aagatacata aataaaagat tgagtaaaat ttattttata gtagataaga
    34441 tgtcacaaga aaactacttg aaggacacta ctgagtcaga agaaactggt tatctagtag
    34501 aatataatga atactttaaa gaaggcatat ttagtaagat aaataactgt ctatatgagc
    34561 ttaatagaag tttaaagata aagtttatta aattagaaaa agaaaaagaa agtgtaaaat
    34621 cattagtaac agatatttcc catcaattaa aaactccttt agcatcctta aaattataca
    34681 atacattact tatagaagaa gaattagaag aagatgaagc caacgagttc ttattaacca
    34741 ataaggcttc tataaataaa ttagaaaatt taataaattc tttagtgaac atatcaaggt
    34801 tagaaattag catgataaac ataaaaaaag aagataatga tataaaaaat actattctaa
    34861 atgcagtaaa aagtgttacc cctaaggcta agagcaaaag tataacatta aatttaaatg
    34921 aatttaacag tagaattata ccacatgata aaaagtggac tgaggagagt atttttaatg
    34981 tattagacaa tgctgtaaag tatacacagg taaatggaga aataaatata tctgtagaag
    35041 aaactgctaa tttctttaaa attataatag aggataatgg catagggatt gaaaaaagtg
    35101 agtttaataa tatatttaaa cgtttttata gaggttctgc tgatgaaatt gaatctgtag
    35161 aaggtagtgg ggtaggattg tatttaagta gaaagatttt agaagaacaa ggtggaaata
    35221 ttatagtttc ttcaaaagta ggaataggga gcaagttttc tttgtttctt acaactatgt
    35281 aatattaaat gaaaggaaaa tgtaagcctc tacatttatt atataagtat aaacagctcc
    35341 agaaataaaa aattaataaa atatggagta atataactta gaatgagagg ataattttat
    35401 gaaaatacta tatactgaaa atttatcaaa gcattatgga aaaggagaaa gcttagtaag
    35461 agctttggac aatgttgact tagaaataaa tgaaggtgaa tttgttgcaa taataggaaa
    35521 atcaggttct ggaaaaagta cacttttgca tatgattgga ggtcttgata tacctacatc
    35581 tgggaaggtg tatatagata ataaaaatat atttacatta aaagaagaag aacttgctgt
    35641 atttagaaga agaaaaatag ggtttatatt tcaaagctat aatttaattc catctcttaa
    35701 tgtatgggaa aatgtagtgt tgccaatcgg tttagatggt agagaggtag atgaaccttt
    35761 tataaaagag cttttaaaat cattaggact tgaaaataaa cacgacgttt taccaaatac
    35821 attatcaggt ggtcaacaac aaagggtggc aatagcaaga gcattagcta caagaccagc
    35881 tataatactt gctgatgaac caactggaaa cctagattct aaaacatctg atgaggttat
    35941 gagtatatta aaatctatgt ctaagaagta cagtcaaact ctagttatga taactcatga
    36001 tgacagtata gcccaaatgg ctgatagagt tatattcata gaagatggca gggtgtcaaa
    36061 ggttggtgat aaaaatgatt aatactacta aaatagcaaa aagtaattta aagcaaaata
    36121 aatcaaaaag tattctaata ataattacta taatactttc aaccttaatg ctttcttcta
    36181 ttggaattta tattgttgat gcaggagcat atcaaaaaga aaatactata aagtattcag
    36241 gtaattatca aggtatatta gcaaatgtag acgaaaaaca agctgatata ttgagtaatc
    36301 atgcagatgt tgaattaact ggtaaaatga atggagtagg tgttgaaaaa ctggaagatg
    36361 attcaaatat ttctctagca tacatgaatg aagatgcttt gaaattaaat agttttgaat
    36421 ttatcaaagg aaaattgcca tcaaaagaga atgaaattgt gttagatagt ggagctttaa
    36481 aggcacttgg ttataaaaat aaagatttag gtgaaaagat aaaaatatct tataatgact
    36541 ataaaagtga taaaaaaatt gaaaaagagt tcattataag tggaatctta aaaactagcg
    36601 aaatatctga agcaggtaaa tattattatg ctattatatc agaatcatat atgagaaata
    36661 ctagagatat gtcacaagaa gattttaata tatatgtgaa attaaatgat aaaagtaatt
    36721 tatcgataga acaagcaaaa tcaaaattaa ataagattgc taatgatgta ggattggaca
    36781 ctattaatgt agctgtaaat gaaaactata taaatgcttt aaaaccagat atggaaacaa
    36841 ttatgggtgg agtatttgta gggttagtaa ttgtattgtc tagtatttta gtaatataca
    36901 atatatttta tatttcaata gttacaaagg ttcaagaatt tggaaagctt agagcaattg
    36961 gtgcaactaa aaaacaagta aaaaatatag tatttaagga agggttcata ttagccggaa
    37021 tagcaatacc aataggaata attttaggct atgtacttgc aaatattata ataaaatcat
    37081 ttatggatat agatgtaaaa tcatcacgat taccagttat attatcggtt gtggtaataa
    37141 gctttatatc tgttgtttta tcacttttaa aaccaatgaa agtagcatca aaagtatcca
    37201 ttgttgacgc agtaagatat acaggaaata aaataagtaa taagagtaag agaaaaggat
    37261 ataaaaatat aaatctaaag agattatcac atgccaattt agaaagaaat aaaaaacgta
    37321 cttacatgac attggcatca cttatactta gtggaactat atttataaca gtatcaactg
    37381 ccttagaaag ttttgatgct gagaaaatgg caagagaaca ctttccttat gatatagaag
    37441 ttagactatc tggttatgaa atgaacagtg ataaaaatcc taaagataat ttgaatattt
    37501 tacaaatgga taatcctcta agtaaagatt tttttaatca aataaagaac atggaaggaa
    37561 ttaaaagaat agaatctgca agaagtgtaa aaattggtat ggaggactat gatgttgaat
    37621 ttaagtatga ccttttacaa agtataaatg aaaatgatgt aaaatcatta aacaagaata
    37681 taatagatgg aaaaataaat cttgaaagac ttcaaactgg agatgaaata gtaattactc
    37741 atgttgatac agcaaaagaa atgggagtaa aagcaggaga taaaataaga ttaactttat
    37801 atgatggaga taaaaagata aaaaaagaat ttaaagttca agcaatagct atgggggttc
    37861 caagttttgg tatagggaag gattttattg atagaacttt gaaatacgat tcaactagta
    37921 gcttgggaat atatacagaa gaaggaaagt atcaagaaat caaagatagt atcaagaaga
    37981 ttgctaaaaa taatggattt ttagagactg attttataga tagtagaatt gagtcaaata
    38041 aggctactat ttcatttata aagataatgg gatacacgct aacaggcata ataggtgtga
    38101 ttggatttat gaatcttgtc aacacaatga taactagcat agttactaga aaaaaagaac
    38161 ttggtatgct tcaagctatt ggtcttacta ataaacaatt agtgaaaatg cttaattcag
    38221 aggcaatgta ttacacttct ggaatgatga taggaagtat tctatttgga ggtatattag
    38281 ggtatattgc tgttatattt ctaaaaaaga ctggattatc ttatgcaaca tattctttgc
    38341 caatagtacc aatcttgttg atgattgtgt gtattcttat agctcaattt ataacaactt
    38401 atttgatagg acgaagtttt aataaagaat ctcttattga tagagtaaga tacagtgaat
    38461 aaaatacaaa tatttatggt tagtttaact agcaaaaaac atataaaaaa atatagttaa
    38521 agagattcat aattaaatag gttaaagcat agtacagagc aaacaattta agcagatgtt
    38581 tgctctgtat tttattatgt aatcaataat atatctaata tactgatatt attcatgtta
    38641 gaaaatgtac cggtacaatt ctaaaaaagt ataaaattgt accatataaa atgaaaattt
    38701 tgtataataa tcgtatagaa aataaatagg agctgatata tatgaataag aagattaatt
    38761 atgcaccagg accaactgaa actagggaaa atgtaagatt agcaagagca gaaaaaacaa
    38821 caaatccaga tatagatatt gattttgttg aattttataa aaagacatgc gagaagtttg
    38881 gtaatataat tggaacaaaa aacgatgttt acatattgag tggtgaagga atattaggtc
    38941 ttgaagcagc atgtgcatca cttacagaaa aaggagatag agtacttgta attgacaatg
    39001 gtatatatgg agaaggtttt aaagattttg taacaatgta tggtggggag tatgtgcttt
    39061 tttcaagtga atatactaaa agtatagata ttgatgaatt gaaagcattt ttagatgagg
    39121 atagtaattt taaatatgca acagtagtgc attgtgatac tccaactggc gtattaaatg
    39181 atgtaagcaa aatatgtcca ttacttaaag aatatggaat attaactgta gttgattctg
    39241 tagcaggtat ggtaggagaa agattaagtg tagatgaatc taaaatagat ataatattag
    39301 gtggttcaca aaaagctatt tctgcaccag caggacttac tatagtcgga ataagtcaag
    39361 atgcaaaaaa ttgtataaaa aatagaaaaa ctgatgtaat aggtttttat tgtaatttaa
    39421 atatatggga aggctattat gaaaaaaaat acttccctta tacaatgcca ataagtgaca
    39481 taatggggct tgataaagca cttgataata tattagaaga aggaatagaa aatgtattaa
    39541 atagacatga aaagatagct tatagtgtta gaaaatctat tgaggaatat ggtctagaac
    39601 tatttttaga agaaggatat tctaatacag tcacagctat aaaaattcct gaaagtatag
    39661 gagctttaga acttactgat tatatgctta aaaattataa tacacttgtt gcaacatcac
    39721 taaatcaata tatggatact attttaagaa taggtcatat gggagagaat gcaaatctaa
    39781 ataaaataga acatgtacta aatgttttgg ataaaagttt aagtgcatta ggattcaaag
    39841 gaaatggaaa tttgttaaat ttatttaata agtattattt ttaatagata gggggatata
    39901 gtatggaagt tacagctaag caaaaattta caacgaaaga catggtggaa acctcactat
    39961 taatagcatt agtatttatt gcaactaaat ttataaacat aagattacca atatctataa
    40021 atggtgggct tgtgcattta ggaactgcaa tgttgttcat atcagcaata gtatttggaa
    40081 gtaaaaaagg tgctttatca ggagctattg gtatgtcact ttttgatttg atatctgggt
    40141 ggactttatg ggcaccgttt acctttatag ttagaggagt tatgggttat ttacttggaa
    40201 agattgcatg ggcaaatggt aataatggga ataatttttt aatcaatgta ataggaatat
    40261 gtgtttcatc aatttggatg ctatttggat attatgtaac agaagttata ctttatggta
    40321 attttatagt acctcttact tcaataccag gtaatttaat gcaagtattg ataggtctta
    40381 taatagcgct tcctatttct aaggtattga aaaaatgtat aaaataaata taaagtgaac
    40441 taaaatttat gatagcataa attatactgt ataatattag gatggagtac ataattaaaa
    40501 aattttagta tagaggttag ttcacaatgt tatcagttac aagatattca atcaaaaatg
    40561 ttcgtttaaa gccgtgggta aagtttattt ggtattttga gacaaaatca aatatactgt
    40621 taaataataa gttacttccg acagacagta tagatattat tctcaatctt tctgatgtta
    40681 tggaatataa aattgaaaat caagactata ttgctagtaa tattcatttt aatggaataa
    40741 gggataaaca tggatttatt attcaacatg gaaatatacg tgttataggg atttcttttt
    40801 atccatttgg tctctatcca tttttaaaga taccaatttc agagtttaac agacaaattg
    40861 ttgacctaga agcagtatca aaactatttg caaaaaaatt agaagagtca ttaaatccaa
    40921 caaaagcaat agaaaaagtt gttttatgtt tagaagaagt attattatcc atattagaag
    40981 aagatttaat ttcaaataaa tatgtaaagc ttctaaattc atttatatat gttaatagat
    41041 atagcaatat aaaagcattt tgtgatgata caaatataaa tattaaaaca ttggaaagaa
    41101 tctgtttaaa atatacaggg tatacaccta agattttaaa gagaatttac agatttaaaa
    41161 tggcaagcaa tcaacttatt tataattata aagatgatga actttttgat tttatttatg
    41221 aaaatgagta ctatgaccaa gcacacttta taaaagaatt taaaaaattt tcagggacgt
    41281 caccaattaa atttagtggt gagaataaga cgattaaaga aaatataaag tacagctatt
    41341 tataattata ctgtcgattt tttacaatac ctccaattta ttttttgata tgatatagga
    41401 aaataaattg gaggttttac atatgaaaat tattacagaa atagttgagt ttaatgtgga
    41461 agaaaattta tcaaaagaag agtttataga aattgtaaat gacttagaat taaaatttca
    41521 ttctattcaa tctggtttta ttgatactga gcttttatat gatgaaaagt ctcaaaagtg
    41581 gattatgata caacattggg aatctataga agacctcaag agtgtttcta gtaaaatgtt
    41641 taaagattct tcaactgaga aatttagaaa agctttaaag aatcaaactg ttaagatgaa
    41701 tataatacca caaattaaaa gttggaaatt atagaactga atgtaaaaga tagttaaata
    41761 gtatcaaact aaattagcca aataacattt tgtatttaaa ttgttatttg gcttttagtg
    41821 tttattttaa tatgagtgtt atatactaga aaaaccaaca aactgttaga gtgtggatta
    41881 tcataatgtc atattcggga gctaaaaggc ttaatactga tttgccaatt ggtcaatcca
    41941 tgcatgtcca tctggatatg ctgttattaa agcatttaca agccaatccc tttgctcttg
    42001 tgataatagt gggactgtta catcgaagtc tttttgataa ccaagcctat ctatgtatat
    42061 tgatttgcaa aacaaaacca cttcgggagc catatatggg atattatctg atgttatcag
    42121 tctggcctta tccatatctc ttataattct ttgattttgg gaaaaaagaa attttccatc
    42181 tttctgtgga ttatatatca attcaataaa atcgaactgt aactgttcgt cacactgtat
    42241 attcatttta tagttatcat cctcgacttt ttttaattca ataaatgagc aatcaggctt
    42301 aatcgcaaaa acacaaattt gctctatatg ttgcttgtta aggtcgataa taggtcgtaa
    42361 taaataatta cccaagggct catatatatc ccatccttga ttaagcatat actgaatgct
    42421 cttttcttta tctgtgtcag aaatccatat gtcaatatca ctgtgtgggc gaatatgcat
    42481 attcgcaaat aattctaggg catatccacc acatattgaa tatgaaaagc cacaatcatt
    42541 aaaaaagttt acacaatctt taattagttt gttcatagca acctcctttt gcatgtactg
    42601 aaataaatta aattttttaa aataccaact aaatgtttgt tttgtgtaaa tataaaactg
    42661 ccgatattaa ggcagtttta tatttactat ttagaaaatt tattaacaaa tttttgatat
    42721 tcacacatta tcttatcatt cataaaagaa atacccataa aagcagcttg ctcaccaaac
    42781 aaagtaaaac gttctatcat ctgttgtgga ttacctggat aaactgttgg aatacaccat
    42841 gtgtttagaa gaaaaccaaa catttgagca gcaatctctg gatttttaac ttccatagaa
    42901 ttatcttgat ttccttcttc aatcaatgct tttatccaag gcacaatatc atttataatc
    42961 tcatcaaact gcgtagctaa atacttagga ttacgcaata gtgatgatga cattaaaaaa
    43021 atgtcttgtt tattgtgctg ttcataagta tgaagcaacc tatattgtaa tttttctaat
    43081 gcggtctttc cttcatcatt ttttacctca tacccataaa atccatcatt atacaaccga
    43141 cttgtgattg ccttaattat ttcgtctttt gatttaaagt gatgataaat agcacctctt
    43201 gaaaggccat ctaaattatc tacaatgtct tgaatcgttg tctgctcata ccctttttct
    43261 aagaataact tatgtgaaat atcaagtatt ttttgtatag tattatcagg ttcttttttt
    43321 cttcccaaaa aactcactcc tcgaaacaat catttggtat gtttatctta gcaaatacat
    43381 aatgatttgt caatgtgtct ttttagcatg ttgaacaggt atattgccat gctttcatta
    43441 ttttattttt ttgccttata ctggactata tttgcagttt cctaatatat ttggctcata
    43501 tttaaatacc attatctaaa agcactattg ctgtacgaga ggcaataggc attttagaag
    43561 aaggaggagt tgagctaaag tatacatcat aatctcttaa gttttctttt tttgtgtttc
    43621 ctccaaactc taccatatca gtatttaata tcgttttgta gtttgtattt tgaaactttg
    43681 aaatatcaac tgattgtatt agattagggt ggaaattaaa tataaataaa tatttgccct
    43741 tactgtacac caaaatcttt ttatcattat ccatatgtat aagttcatga ctattttgga
    43801 taaaaatatc actgtatttt gtaagttcga gcatagattt atcaaaattt aataattgtt
    43861 tatactttaa gttgttatct tctgaaaggc tccattgtct tctggcatac ttatagctcc
    43921 aattgttgcc ttctcgtgga aaatcaatcc actcaggatg tccaaattca tttcccataa
    43981 aatttagata tccttcacct gctagagaaa aagttattaa tttaattaat ttaataagag
    44041 atattgctct attgataaca tggttatttg agtttatttc catattccag tacatttcct
    44101 tgtcagctag ccaaaaaata attgttttat cgccaactaa tgcttggtca tgggattcac
    44161 aatatcctat atttttttct ccaggtcgtc tagtagtaag ttcataccac attttgccca
    44221 aatcccagtc ttcatcagat aagttagaga tagtttttat ccaaaaatca ggtactccca
    44281 ttgcgagtct atagtcaaat ccaattccgc cgtcttttat agggatacac ataccaggca
    44341 ttccactcat atcttctgca atacttatcg aattaggttt aatttctttc attaattcat
    44401 ttgcaaattg gagatatgtt atagcttcaa tatctgtatt catactaaaa tatttttcat
    44461 aactatcaaa tgaaactcca agaccatggt tatgatataa cattgaagtt acaccatcaa
    44521 acctaaagcc atcaaaatgg tactcattta accaaaattt tatatttgaa agcaaaaaat
    44581 gtattacttc aggtttacca tagttaaaaa gttttgtacc ccaagcagga tgatttccct
    44641 ttgaacctga gtggaaaaat tgatgttcac ttccatcaaa ctcatttatt ccttctaaag
    44701 tattctttac agcatgagaa tgaactaaat ctaataaaac agcaatcccc attgaatgag
    44761 cagtgtttat gaggtttttt aagtcttcag gagtaccaaa cctagatgag attgcataaa
    44821 agtttgaaac ttggtatcca aaagatgcat aatatggatg ttccataata gccataagct
    44881 gaatggtatt gtatccagct ttttttatct taggaagtat tttttcagta aattcattat
    44941 aagttccaat agattcgctt tcagtagaca tacctatatg acattcatag attagtggag
    45001 aagttatgtt ttttaagtca aaatcattgt cagtccagat aaatggagtt tttggttgcc
    45061 atatttgtcc attaaatcca ccaaaatctt tttgaattac tctttttata tataatggta
    45121 tacggtcaaa tgtttttcca tttgcagtaa cttgtacctt tacctctgat ttgtgaggta
    45181 agctattttg accaggaata aatatttccc aattaccaga cccaattttt cttagaggat
    45241 gagattttct attccaatta ttaaagtcac caattagtga caaactatct gcatttggag
    45301 cccattccct atagaagaat ccatcatttg tctgatgaaa tccatagtaa agatgagcat
    45361 ttgcaaaaga actaaaatct ttatagtttt tcaatgtttt tgatttcata tcctcataat
    45421 gtttcattcg cttttttata tcagattcaa atggttgtag gtatacatca atatctaaaa
    45481 ttttaaatct tttttgactt ttcattgtat ttctcctcca taataaatat tacatctaaa
    45541 aaataatatt tatctcatac tatcttctct gtataattga tatatattat ataatcctaa
    45601 taaaaagctt aagccaccaa tgattaattt cataatttac ctccctagta tttatactta
    45661 tatatcgtat tcatcaaaat ttaaaagttt gtttaaaact cttttaataa tatcataatc
    45721 aaagccttta taggaaagat gttgagaaat tttttgatat attttctttt tatcttcttt
    45781 ctttactctt tcataccttt tttttgctag atacatagca ttttcaaatt cagtgtcctt
    45841 gtctaattca gatactgctt cattgattgt acttctattg atacctttat tatataaatt
    45901 ctgcttaatt ctgtttttgc cacatttatt taggttaaca tttgtgttaa caattttttg
    45961 agctaataca ctgtcattta cgagattata acttttaaga aaatctatga ccctctcaat
    46021 tatattttca tcaaattctg atgatagttt ttcttttaat tttttttcag attggtcagc
    46081 atgagataaa atatttaatg ctttattttt agcttttata tacatttcat catctaaaat
    46141 aggttttaat tcttcttcat ttatttcatt cccttttttt aaattaaatg tataaaccag
    46201 ttctttaaat atagcaataa aaaatttatc atctacataa atattaactc tatcatcatt
    46261 tcttttttgt tgttctattt ttgtaattat acccataagt cctccttttt tatatgtaat
    46321 tatattaaca aaagcaacta ataaaattta acaaaaaact tatatttgta tgttatattt
    46381 tatcaaaatt aagacaaaat agtaaaaagt tatttttatt aaaaagtaaa gcagaattat
    46441 gttaaataat tattttttaa agtgtttttt agcatataaa tataattata tacccaaaaa
    46501 aacaaattat aagctttaag aaataaaaaa taatggtaca atagtattat aacattaaaa
    46561 ggagacaaaa tatgagaagt gaaaatctag ttaagatggc agagttgaaa aatactgaaa
    46621 aactagagaa atttaatcgc cataaaggca aaatcaaaat aggtatattg ggaggtactt
    46681 ttgaccctat acactatgct catttagcaa cagcagaatt tattagagac aagtatgaca
    46741 ttgacaaaat aatctttata ccatcaggaa atccaccaca taaattatgt atcactacag
    46801 ataagtatga tagatataat atgacacttc tagcaactga aagcaacgaa gattttttag
    46861 tttcaaaagt agaaatcgaa agaaagaaaa ggacttatac aatagataca ttaaaatact
    46921 taaagaaaaa atacaaaaat gcagacattt attttataac aggtgcagat gcaatttgta
    46981 gtgttgaaga gtggaaagat gtaaagaaaa actttgagtt agctactttt atagccgcaa
    47041 ctagaccagg aattagtttg ttaaggtcac aagagactat agaaaaacta actaagaagt
    47101 ataatgctga cattataaca gtttatgttc catcactgga tatatcgtcc acatatataa
    47161 gggaacagct aaatgaaggc aagtcaatac gttacttagt acccgaaaat gtagaaaatt
    47221 atttatatga aaataaacta tatcaatatg gagatgatta aatgaactta gaaaatatta
    47281 accaaagact aaatcaaatg ctaccagtgg ggaggcttag ccattcaaaa aatgttgcaa
    47341 aatgtgctga aaaattatgt gagatttatg gatgtgacaa agaaaaagct tacttagcag
    47401 gaatgattca tgattgtgca aagtatttaa gtgacaagga aatagaggat tatgtaaata
    47461 agtatgaaat atatttagac ccaatagagg atggaaatcg ttccttgtct catagtgtaa
    47521 ttggggcgta catatgcgag tatgaatttg aagtggaaga tgaagatatt ataaatgcaa
    47581 tcaaatatca tacaacaggg agagaggaca tgtctctttt agaaaaaata atttacatag
    47641 ctgatttaat tgaagaagga agaaaattcc cagttgtaga tacattaaga gaattagcat
    47701 atggagggaa gcttgatgaa gcacttttaa cttcttttaa taatacttta atgtttgtca
    47761 tcaataaaaa agaagagata catccaagaa cagttatggc aagaaactat ctaattaaag
    47821 aaaagttatt ataaaaaagc tatataaagg caaacatatt aataaattta aatttgacta
    47881 tgttacaaaa tcgtattaat atgttacaat aactaagtgt attttaatta ggaaagtgag
    47941 gaatttttat atgacagtgg aacaaatgac aaaaatagca tatgatgcca tagaagataa
    48001 actgggtcaa gatacagtta taataaatat aggaaaagtt tctagtttgt gtgattattt
    48061 tattattact acagcatctt ctcagagaca ggtaaaagct attgctgaca atgtggaaga
    48121 tgaattagca aaactaggat tagagccaag aggtaaagaa ggacaaggaa ctcaaacttg
    48181 ggtactcctt gattatggag acataatggt tcatgtattt aatgaggaaa atagagggtt
    48241 ttataattta gaaaaattat ggaaagatgc accttatata gatattgaca cattagcata
    48301 aacatgataa tatatgttat tataattaga aaatttcaaa aattatatta aattgaatag
    48361 actagagcag taaaagatta tattttttca gagagttagt ggaaggtgag aactaatatt
    48421 atataacttt gaacttacta gattaaaatc tattctccat ggctggatta aaagctatct
    48481 aagagagtct atatatgtag taggctaatt agggtggtac cgcgaagatt tatcctcgtc
    48541 cctaaacgta agtttagtga cgaggatttt ttattttcaa agaattattt ggaaattata
    48601 agtaaataat actaaataag gtagttatac acaattaaaa ataggaggca aaatatgagc
    48661 gtatataatt ttaaagaagt agaatcgaaa tggcaaaaaa tctggaaaga taataatcag
    48721 tataaaatgg atactgcaca aactgaaaaa cctaattatt acacattaga aatgttccct
    48781 tatccatctg gaaaaataca catgggacat gttagaaatt attctatagg tgatgttgtc
    48841 gcaagattca aaaaaatgga aggatataat gtacttcatc caatgggatg ggattctttt
    48901 ggattaccag ctgaaaatgc agctataaaa catggaatac atccacataa atggacaatg
    48961 gaaaatatag aagaaatgaa agagcaatta aatttattag gtattagtta tgattgggat
    49021 aaagaagttg caacttcaac gccagaatac tatagattta cacaagaaat atttttgaag
    49081 tttttagagc atgggcttgc atataagaaa aaatcatatg ttaactggtg tccttcttgt
    49141 gagactgttc ttgcaaatga gcaagttgtt caaggagcat gtgagagatg taaagcaaca
    49201 gtattaaaga aagatttaga acaatggtat tttaagacta ctgaatttgc agaagaatta
    49261 cttaatgact tagatatatt agatggatgg ccagaaaaag ttaaaacaat gcaaaaaaat
    49321 tggataggta aaagtacagg agcagattta gtttttgata tagatggaac tgataaatct
    49381 atgacagtat ttacaactag accagacaca acttatggag ttacttatat ggtacttgct
    49441 ccagagcatg aattagttaa agaattggtt gcagggactg aatatgaagc agatgttgaa
    49501 gcttttgttc aaaaaatgca tactatgaca gaaatagaaa gaacagcagc tgatgtagaa
    49561 aaagaaggaa tgtttatagg tagatatgtt ataaatcctt taaatggcaa gaaagttcct
    49621 ctatggatag ctaactatgt attagttgag tatggaacag gtgctataat ggcagttcca
    49681 gcacatgatg aaagagatag agattttgct gaaaaatata atttagacat aattgatgta
    49741 ataacagaag ataacaaaat gataaattca gaagaattta atggattaga tgcttcagaa
    49801 ggatttgaag gaataatcga taagttagaa aaagaaggta gaggaaagag aacaattaat
    49861 tatagactta gagactggtt agtttcaaga caaagatact ggggttgtcc tatacctgtg
    49921 gtttattgtg atgaatgtgg aatagttcca gttaaaaagg aagatttacc agttctttta
    49981 ccaacagatg ttgaatttac tggaaaaggg gaatctccac ttacgacttc aaagcaattt
    50041 atgtcaacaa cttgtcctca ctgtggaaaa ccagctagaa gggaagttga tactatggat
    50101 acattcgttg attcttcttg gtatttctta agatatgtgg atagcaataa tgaaaatgaa
    50161 ccatttagta aggaattagt taatagatgg catccagtag accaatatat aggtggagta
    50221 gaacatgcta taatgcattt actttatgca agatggtttg taaaggcatt taagagtatg
    50281 ggaatggtag acttcaatga gccatttaaa aacttactta ctcaaggaat ggtacttatg
    50341 gatggttcta aaatgagtaa gtctaaaggg aatacagtat ctcctatgga tataatagat
    50401 gagtatggag cagatactgc aagacttttc gtattatttg cagcaccacc agaaagagat
    50461 ttggactggt cagagcaagg tgttgatgga tgctttagat tcttaaatag ggtttataga
    50521 ttggttgacg agttagcaga tgtggttaaa aaggatgtag agtttggaga attaaattcc
    50581 caagataagg atatgagata tactatacac tctacactta aaaaagttac tgctgattta
    50641 agtgaaaaat tcggatttaa tactgctata tcagctctta tggaattaat aaatgatatg
    50701 tataaatata aagagttaga taatataaac gaagcagtta taaaagaagg tgtacaaact
    50761 atagtaacta taattgcacc atttgcccct catttaggtg aagaactatg gactatgata
    50821 ggtaaagaag gaagtgtatt tgacatagac tggcctaaat atgacgaaaa agctttagta
    50881 aaagatgaaa tagaagttgt tgtacaagtt aatggaaaag ttagaggaaa attaactgtt
    50941 aactcaaata tatctaaaga tgagatggaa aaggtagctt tagaagatga aaaaataaaa
    51001 ggattagttg aaggtaaaac tatagttaaa gttgtagctg tacctaaaaa actagtaaat
    51061 atagttgtta aataactcaa aaatatgaac aaaaatataa atttataaaa taaacgaggc
    51121 tgtctcaaaa tagagataag ccttttttca ttgttgaaat tataaaattg ttgaaattat
    51181 aaaaatagat ttgattttat acaaaaaaat gattaaagaa ctaaaaagtt cattcttata
    51241 taccctagtt gttgttttaa atattattat aattaatgtt aaatcgagtt aatgtattaa
    51301 gtggtatgta aatcattcag tttccaaagt gttgttcatc aaggaactgc tctaagctta
    51361 aacgaaaaaa atataattat tgtattttga aatttttttc tatataatga aaacaggcaa
    51421 aaaaggtgcg tatctatgct ttatatgaaa cacccagaca acgaaattca gattcatata
    51481 atagctacca tgtttctaaa aaggaacttg taaaaaagat agcgtttcaa cttcccagtt
    51541 gattaaaatt atcagcagga aaacaagtat ggtcagcagt ggtattctaa tgggtgttgt
    51601 cgatatatcc aagttgggat tgtctgagga tgttatcaaa taacttgtga taggtgcagt
    51661 agatgtggag attcctgata cggatttatt ctcaggggat ggttgcttcg ggagatatgg
    51721 agcgtaacca gtttattgat atggtaacca ttttttgtct aatttgatga aagaatccca
    51781 agaataccat acagaagtaa gacaggaatt aactggtggt ataaactgat aaaggcaaat
    51841 ttaaaaattt tgataggatg attttaagat ttatcttcta ttttgtgttt tagaaataat
    51901 tgaaatgtta tgaggaggta tgtacaaatg gagactgtaa caaataatca atcaggtgta
    51961 gaaattcaac tcagtctgtt tgaagctatc aaagatttat tgttaattaa tcctattttc
    52021 ttgttgggaa ttatcttgat tggtattttc ttagttgtaa tattcaagaa aaatagtgat
    52081 attccaaagg taaaaacatc tattttatca ctggcaatgt attactactt atgtttaaca
    52141 ctatcgcata ttgttggtat ccctacattc agtgaatata taagactgtc acagttaggg
    52201 gaaacgttct ttaatccaaa cataaatctt attcctttta gtgatggatt tagtttgagt
    52261 tttattttga atattttact ttttgtccca ttaggttttt tatgtccgct tatcagcaaa
    52321 acctttgaac gtttgagaaa tacattcttg attggttttg gattatctct ttttatcgaa
    52381 actattcagc tttttacgtt acacagagca accgatatag acgacttact cacgaatgta
    52441 attggaacaa taataggcta tttctgcttc aaactgattg ctaaattgcg aattgtaaaa
    52501 ctacattcag atgatgactc tatggaacag gattacacag tatgtattcc gattgccatt
    52561 ataatagttg cccttatatt aggatttttt agttaagttc caaatagcta atttgggaat
    52621 tatggaggtc attaagtgaa taaaattact tttatatgtt taggtgtaaa aagtatggag
    52681 aagtcaatta aattttatag agatggatta ggattttaga ttacaaactt ttagtttacc
    52741 aaggagatag caaagttgtg tgccaaaggg taaccatttg gaaatccaga caacataaca
    52801 gatagtacgt taccttttac aggagcatac tatctgttat attgtcaaca tcctatttta
    52861 tggtttgtgt gtagtttcct gtaaactgaa ctaaataatt aaatagaagc tattagaatt
    52921 aataataaca gatagatatt aataagcata aacaaatagt aaaatgtgta gatatagacg
    52981 tttttaagtg aaactattat aaaataattg actgataatc aactgacaaa acctattttt
    53041 ttaggtttaa ctgacattta actgacagtc agtttttact atttaaatag aagttcgctt
    53101 agattgtcgg aagcttcttt gtccatttct ttaagtacat gagaatatct attcatagtt
    53161 atttttatat ctgtatgacc caatctttca gaaacaactt tcatattagt tccccctaaa
    53221 agcagcagag tggcacttgt atatcttaaa tcatgtattc taatcttttt taaattattt
    53281 tttttgtaaa attgtgaaac gtttttctta gtgaagaagg tagaaacgga tttaaattgg
    53341 tgtttagaca aactaaatta tttctgtttt ccaggatacc ttacaatttt aatttgcttt
    53401 gtcttagttt tttaattttc aatttttcaa ttaattcttt tggagctgat aaagctcttt
    53461 ttgattttgg tgtttttggc tctttaaaag atattttgcc attagaatag acaagaattt
    53521 gattaacatt tatcagattg ttatctaagt caacatctga ccacctaagc ccagtagctt
    53581 ctcccattct taaacctaga agtaacatta aaagaatagg tatttctata ttattactaa
    53641 gtaacttttc tattagcaat aatacttctt ttctattgta tatttcattt gtagaatatt
    53701 tttctttaga aggaagtttt ataaaatcac atgaattttc ttgtatttct cttaatctat
    53761 atgcttccct tattacagaa cttagaaagc tataccttac tttagcacta gtaaaggtag
    53821 aattattaaa gttataattt acaaaagctt gtactgaact aggatttact tctgtgagtt
    53881 ttagattctt aaaaagaggt tctatgtaat ttttaaccca agattttgta tttgtaacag
    53941 tatatggaga ccaattattt ttattcatgt ccatgtattt ataacatctt tctacaaatg
    54001 tcatatcttt tggagcaaca aacttattat tgtttatagt agattttatt tcaattaaat
    54061 gtttttcagc atcctttttc ttttcataac ttccataact tttctgtttt atcttgccag
    54121 ttgtttcctc tatgtactcc acatatacat agaatttttc ccctcttttt cttataaaag
    54181 ctgatttgat attcatatat gtacgctccc tttataagtt agttttataa aagaacagtt
    54241 gtactactct aattatgtca ttatcttttt aattctttac agttacatta taacacgtat
    54301 ttgtatacac ttaaattaac aggaagataa atatcgttaa aggaggaaaa gaaaatttaa
    54361 tgtagaatta ttaagatata tagtatttca tatataaaag ggggagatat tgtgaaatta
    54421 aagaaattat tatcactagg gttagtgtca atgtttgttg ctggttttcc tatatcagct
    54481 aatgcacttg ataaaattga taaaattcaa ggagcagata aatacgaaac tgctggtatc
    54541 atagctgaca aacaaagtta tactactgca atacttataa atgcagatag tacaatggct
    54601 gatgggctaa gtgcgagtgg acttgcagga gcaacaaatg caccaatcct tttgactaaa
    54661 aagaataata tacctaatgc aactcttaaa agagtagaaa aagctaaaaa ggtatacata
    54721 ataggaggag aaagttccat tgataaagca acagaaactt ttctaaaaga taaaggaata
    54781 gagactagaa ggcttcaagg taatgatagg attaagacaa gttataatgt agcaaaagaa
    54841 ataaattcca ttaataaagt aaataaagta atattaacta acgctttcaa aggtgagcca
    54901 gatgctatga gtgctgcacc tgtagcagtt agagataaag cagctatagt attaactgat
    54961 ggaaaaagtg ttccatttaa tacaactggt atagaaagtt atgcaatagg tggcacttca
    55021 tcaatgagtg atagtttagt aaatgatact aattcaactc gattgggtgg agtagataga
    55081 tatgatacta ataaaaaaat agtaaataag ttctataatg gcgcaaaaga attttatata
    55141 gctagtggta ctgatttagt ttatgcttta gttgcttcac caatggctaa aaataatcct
    55201 attgtactag ttgatattgg aagtaataaa gatatactta aaaatgctgc aaaagtaacc
    55261 gggataggaa atctaagtga taaaattatg ctagagtgtg aaaataccat caataatatt
    55321 agtcttccat cttcaaataa aaatataata tgtggattaa agattgattt tgattcaaat
    55381 cacataattt ggcaagataa aggaagttat cttgaatgtt atgatgataa ccttgaatat
    55441 tatactgaaa aatataaaga cccaacagtt cctataaata tagaaaatac aagtacacaa
    55501 aatttagatg taacaaaatt aaaagtttat ggagaagcta attcaacatt atatatgggg
    55561 caatacgcta aaataaaaat ggattttaat agtttcggtg tattaaagcc aggagaaaaa
    55621 tgtactattt atttagatat aatttcatta gatccatcaa gaagcatgac attaacattt
    55681 tcttatgtag gtgataaaga aaattataaa tatgaacaag gattggcaag tagaatatta
    55741 ttttataacc cggatggaga agattttttt gaatataaat aactagtggg tatgtaaatg
    55801 tctcgatatt gctcatatgt tgagttatta tatttagttt catatgaact atatggattt
    55861 taaaattaaa aatacttaaa aacatttatt ttaatagtag gtgttttttt gattaaaaga
    55921 atattattat aatgtaaaaa gtaataaata ggtaaaatat gtaagaatta tatgttataa
    55981 tatttttagc aagaagatgt aatctacaat ttatagagtg gagttcatgt atcaaaaaat
    56041 tatcctcccg acgttaagaa gggaggtgaa tatacatgga taatttttta caaggcatac
    56101 tagcaagtct atctgctagc ttaatagttt atatagctag caaactattt agaaagcgta
    56161 aaaaaccact caaagcggca actaagagtg gttgggaatt tgatttaaaa atcagattcc
    56221 ataaaactaa gtaatttcta aattatgaac tccactctac agcaaaatag attgtagttc
    56281 ttcttgcttt tattatatca caaattggta cagatattca aaaataatat ttttatgata
    56341 taataaagtc atagaaattt tgcagtgttc gattttttga agaaattaag gcttaacagt
    56401 tgaaatataa ggcattgagg acttgtgata agtgttatca attgcactac tcatggttca
    56461 ctgcaaattt gagagatttg tatatgtgta ggcattggaa atactcaatt tattttgggg
    56521 ttttatatta actatatgga atgtaaattt catattccat gcattctgct tacacttaat
    56581 agaaaagttt tatattaact atatggaatg taaattattt ctagcgactt ggtcggttgc
    56641 tccatttagc ttgttttata ttaactatat ggaatgtaaa ttttttcaaa tctgatatat
    56701 cgaccttaaa tttcgtgggt tttatattaa ctatatggaa tgtaaatgat ataaactttg
    56761 ggagttcaga agaaggatta aaaagtttta tattaactat atggaatgta aattagagat
    56821 gaactcagat agaaaaacat gtagatgtgt gttttatatt aactatatgg aatgtaaatt
    56881 cttcaacagt tgtgttacca tctcctacac gcacaggttt tatattaact atatggaatg
    56941 taaattgcta cgtaaccagt ttcaccgctt tcagtatata acgttttata ttaactatat
    57001 ggaatgtaaa ttgttggtat agtttcagaa aagaaattcg aaacacttgt tttatattaa
    57061 ctatatggaa tgtaaattgt atccaaaaaa ttacaataat catacgaact gctaatataa
    57121 gcagtcaata tatgtgtaca tttatctatt ttatctctaa atatataaat tattatcccc
    57181 ctaaaaaact ttcataaaaa caaaaagaac aatatctctt tggcaactgg ctgacataac
    57241 tcataagata tttatataag tcttagtctc tatagctttg cgtcactaga tttctctagt
    57301 tttgccgatt tagttttatt ctataactaa ataatacaat agaattagtt attattcaat
    57361 aagattgttt gaaaattgaa taaatttata ggtatgaacg taatttcaca cttaaaaaac
    57421 agactaaata cgtagaatat atagaaaaat ttacctgtag tttttgtgat aaaaagctcc
    57481 tattattaat ttaagctaga taagtttgag gaagtgacat aaattgaaag aaggaatata
    57541 ttatgttgaa taaaaaatta ccagatgcag aattaaaaat tatgaaatac atatagaata
    57601 ctggttataa aactgtaata tcaaaagatg ttgcagatga aatagaaaaa acatatgctt
    57661 ggaagcatac aactacaatt acacttttag ctagattaac taaaaaaggt tttttcatat
    57721 cgcagagaat tgggaaacat gtacactata gagtattaat aaaagagaaa gaatatctca
    57781 aaatagaggg aaaaagaatt tttggaggtc tacataacaa tccattatca gaattaattt
    57841 caaaattgca tgatggagaa gaaataactg aagaaaaaat aatagaaata ggaaattgga
    57901 taaaaagctg gaaagatgaa gattaataaa taaaaggtag ctactaatat aagtgctacc
    57961 ttttttcttg tggataatat tggggataag ttgtggaaaa cttgtggata aaatattttg
    58021 ctgacattta actgacataa gtattagatt ttgatttaaa aatgttgaaa tatcaataaa
    58081 taattaaata gaagttatta gaattaacaa tagattcctt tttcaaagaa atctttgatt
    58141 tcatcaaggt tatcagtttt tccaagtgct aagataagtt ttattctagc tttttgacct
    58201 ggtaagtccc caccaaatat acaaccaaga ttttttaaat ctctaccaga accaaagtaa
    58261 ccataactat cgaatactct acctgaatga catctagaaa ctataactac aggaagtcct
    58321 ttttctctag catattcaac tccagaaagc atttgaggag gaatattacc tctacccatt
    58381 gcctcaataa ctataccttt ataaccacta tcaacagcaa atcttataaa gtcagcattt
    58441 tctcctacat atgctttaaa taaggcaact ttagattcta ccttatcagt atctataatt
    58501 gttctattta caatatctct tgtaaggaca agttcgttgc agtcaattat tcctaatggg
    58561 ccactagtta gagatttaaa tgtatttaga gaaagagtat ttgttttagt agcttcagaa
    58621 gctaataata cttcattgtt taaaactaca aggactcctt ttcccatagc atctttggat
    58681 actgcagtac aaactgatgc agataaatta cttgaaccat catatccaag ttcagaacta
    58741 cttctcatag cacctgtgac tattacaggt ttgatgttgt caattgtaag gtctagaaaa
    58801 taagctgttt cctctaaact atctgtacca tgtgttatta caacacctgt aatatctttt
    58861 cttgctaata aatcatttac ataatttttc aattccatca ttctataagg tgttatgtga
    58921 ggtcctggaa tttcatcaaa attaaaaact tcaacatctg caactttatc aatgttggta
    58981 accattgata gtatttgttc acctgataaa gttggtatag ctgctccgac tttatcatct
    59041 actgtcatag atatagttcc accagtaaaa actatggcaa cttttttctt aatactcata
    59101 ctattctctg cctttcataa ataaaattta taattccgat aggattatat caaaaaaagt
    59161 agttaaggtc tacataaact cttttattca tataatttag atatttcagt gtaatataat
    59221 tatttcttaa ttttttgcca ttcaacgata taaccattta atatctctct tatggctttt
    59281 cctatcttag tataagcttc atcatataga tttgctagag atattctaat tgaccattca
    59341 ggaccataga atccatttcc actaagtaaa actactgaat agtcattagc taatttataa
    59401 agaacatcta ctggagatgc agttttactt ataaatttaa ataagtcttc tccgtagttt
    59461 aatttagccc attcttcaat atcaaattga gtgtaatatg cagcattgtt tttgttttca
    59521 ttaattggaa gctctaaagc ttcaaataat agtttttgac gtctatggca gatactcata
    59581 ttgagttttt tgtaggcatc tactttgtca atcaatgcaa aagcagagaa aaacgccatt
    59641 tgtacttgct gtggagtaga aagaccagca gtatggttga gagcaacaag tctgctatca
    59701 gcaacaactc tttccataaa tgaaaggcta gaagggttaa gactcatatc actgtaacgc
    59761 ttatctactg atttttttaa ttctccagtt aaatcattta ttttcttatc aaaaacattt
    59821 ttcttatgaa gggcaaatgt accaagtctc catcctgtaa ctccaaaata ttttgaataa
    59881 gaatatactc caacagtgtt gtaaggtagt tttgacatta aggatgaaaa atcttctaca
    59941 aaagttccat atacatcatc tgatattatc attaaatctt tattataatt ttcaattaca
    60001 tctattagat tattacaaga tgtttcatcc atagctatag aagcaggatt attaggatta
    60061 acaacgaata aagctttaat agatttgtca cgaagtttct ctaattcttt ttttgtatat
    60121 tgccatgtat gagcaccttt ttcatcaact tcattagcat ttatatttac cactttaaag
    60181 tcgtaacgag gaaggttagg tatttcaaga taaggtgtaa atattggagt cattaaggct
    60241 atggtatctc ctttttttag aagttcattt gccattaatg aatcaaatat atagcacata
    60301 gcagctgtag caccttcaac agcaaaaatt tctacatcac catatttacc tgatatatca
    60361 tatttcattt cttgaactag gtaatcgtgt actatttttt ccatatgagg taacattcta
    60421 tctggaagtg gatagttatc accaataatt ccatcagcta gttcatgaac aaattcatct
    60481 ccatcaaatc caagttcatt tataccaaag tttataatat ctctagctaa atatgctcca
    60541 ggcatatcta tattttcatt tataaattta aaaaatcttt ctttaatacc ctttttttga
    60601 ggcattccag ccaaatgacc taaatcccaa ttagaacgag tttcagtaat agcgaaatga
    60661 ccaaaagtaa aaaaggcttc tctagcagtt gaacaagtcc agtttggatt tccacgacct
    60721 gcatcaagta attctctgtt attttctttt gcagtgtatt ttgcaatatc tattaatttg
    60781 tccttaaatt caaaagggct tatttttcca tatatgtttt tgatttcttt ataagtatga
    60841 tttttcatgt taaattcctc cttatgtaat aagtttcaca tttttataat aaaataaaca
    60901 agttgcaaaa atgattatac tttaattata ataggtttat agaaaaagta aagtatgaaa
    60961 ttaaaataat aactctattt agccttatta aatatatgtt aaaataaaaa atacctttta
    61021 ataaaaaatg attgttatat aaaataaata ttcaatttaa
//
LOCUS       DAYAOY010000012        58970 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYAOY010000012 DAYAOY010000000
VERSION     DAYAOY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.012"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            183..1259
                     /locus_tag="ACGI6B_000827"
     CDS             183..1259
                     /locus_tag="ACGI6B_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9750292.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1362..2204)
                     /locus_tag="ACGI6B_000828"
     CDS             complement(1362..2204)
                     /locus_tag="ACGI6B_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9750293.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2388..2960
                     /locus_tag="ACGI6B_000829"
     CDS             2388..2960
                     /locus_tag="ACGI6B_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9750294.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3123..3395)
                     /locus_tag="ACGI6B_000830"
     CDS             complement(3123..3395)
                     /locus_tag="ACGI6B_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750295.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3522..4394)
                     /locus_tag="ACGI6B_000831"
     CDS             complement(3522..4394)
                     /locus_tag="ACGI6B_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9750296.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4690..7797)
                     /gene="ileS"
                     /locus_tag="ACGI6B_000832"
     CDS             complement(4690..7797)
                     /gene="ileS"
                     /locus_tag="ACGI6B_000832"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFG9750297.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7890..8122)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8297..8824)
                     /locus_tag="ACGI6B_000833"
     CDS             complement(8297..8824)
                     /locus_tag="ACGI6B_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFG9750298.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8859..9644)
                     /locus_tag="ACGI6B_000834"
     CDS             complement(8859..9644)
                     /locus_tag="ACGI6B_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HFG9750299.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9654..9911)
                     /locus_tag="ACGI6B_000835"
     CDS             complement(9654..9911)
                     /locus_tag="ACGI6B_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFG9750300.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9930..10388)
                     /locus_tag="ACGI6B_000836"
     CDS             complement(9930..10388)
                     /locus_tag="ACGI6B_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFG9750301.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10428..11132)
                     /locus_tag="ACGI6B_000837"
     CDS             complement(10428..11132)
                     /locus_tag="ACGI6B_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFG9750302.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11142..11777)
                     /locus_tag="ACGI6B_000838"
     CDS             complement(11142..11777)
                     /locus_tag="ACGI6B_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFG9750303.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12158..13198
                     /locus_tag="ACGI6B_000839"
     CDS             12158..13198
                     /locus_tag="ACGI6B_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFG9750304.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13661..15019)
                     /locus_tag="ACGI6B_000840"
     CDS             complement(13661..15019)
                     /locus_tag="ACGI6B_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFG9750305.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(15042..15311)
                     /locus_tag="ACGI6B_000841"
     CDS             complement(15042..15311)
                     /locus_tag="ACGI6B_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9750306.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15601..17556)
                     /locus_tag="ACGI6B_000842"
     CDS             complement(15601..17556)
                     /locus_tag="ACGI6B_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750307.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17739..19214)
                     /gene="spoIVA"
                     /locus_tag="ACGI6B_000843"
     CDS             complement(17739..19214)
                     /gene="spoIVA"
                     /locus_tag="ACGI6B_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HFG9750308.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19386..20681)
                     /locus_tag="ACGI6B_000844"
     CDS             complement(19386..20681)
                     /locus_tag="ACGI6B_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFG9750309.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERCKELIEMNNNSNENTTKVS"
     gene            complement(20865..22310)
                     /locus_tag="ACGI6B_000845"
     CDS             complement(20865..22310)
                     /locus_tag="ACGI6B_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9750310.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGC"
     gene            complement(22326..23465)
                     /locus_tag="ACGI6B_000846"
     CDS             complement(22326..23465)
                     /locus_tag="ACGI6B_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9750311.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23784..24434
                     /locus_tag="ACGI6B_000847"
     CDS             23784..24434
                     /locus_tag="ACGI6B_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9750312.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24591..25610)
                     /locus_tag="ACGI6B_000848"
     CDS             complement(24591..25610)
                     /locus_tag="ACGI6B_000848"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFG9750313.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25641..26255)
                     /gene="plsY"
                     /locus_tag="ACGI6B_000849"
     CDS             complement(25641..26255)
                     /gene="plsY"
                     /locus_tag="ACGI6B_000849"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFG9750314.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26290..27615)
                     /gene="der"
                     /locus_tag="ACGI6B_000850"
     CDS             complement(26290..27615)
                     /gene="der"
                     /locus_tag="ACGI6B_000850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFG9750315.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27643..28971)
                     /locus_tag="ACGI6B_000851"
     CDS             complement(27643..28971)
                     /locus_tag="ACGI6B_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HFG9750316.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29209..29409)
                     /locus_tag="ACGI6B_000852"
     CDS             complement(29209..29409)
                     /locus_tag="ACGI6B_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750317.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29411..30343)
                     /locus_tag="ACGI6B_000853"
     CDS             complement(29411..30343)
                     /locus_tag="ACGI6B_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFG9750318.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30388..31320)
                     /locus_tag="ACGI6B_000854"
     CDS             complement(30388..31320)
                     /locus_tag="ACGI6B_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFG9750319.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31375..32664)
                     /locus_tag="ACGI6B_000855"
     CDS             complement(31375..32664)
                     /locus_tag="ACGI6B_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9750320.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32693..33385)
                     /locus_tag="ACGI6B_000856"
     CDS             complement(32693..33385)
                     /locus_tag="ACGI6B_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750321.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33400..34161)
                     /gene="pgeF"
                     /locus_tag="ACGI6B_000857"
     CDS             complement(33400..34161)
                     /gene="pgeF"
                     /locus_tag="ACGI6B_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFG9750322.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34158..34619)
                     /gene="nrdR"
                     /locus_tag="ACGI6B_000858"
     CDS             complement(34158..34619)
                     /gene="nrdR"
                     /locus_tag="ACGI6B_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFG9750323.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34641..34904)
                     /locus_tag="ACGI6B_000859"
     CDS             complement(34641..34904)
                     /locus_tag="ACGI6B_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFG9750324.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35008..35781)
                     /gene="sigG"
                     /locus_tag="ACGI6B_000860"
     CDS             complement(35008..35781)
                     /gene="sigG"
                     /locus_tag="ACGI6B_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HFG9750325.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35873..36595)
                     /gene="sigE"
                     /locus_tag="ACGI6B_000861"
     CDS             complement(35873..36595)
                     /gene="sigE"
                     /locus_tag="ACGI6B_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HFG9750326.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36620..37465)
                     /locus_tag="ACGI6B_000862"
     CDS             complement(36620..37465)
                     /locus_tag="ACGI6B_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HFG9750327.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(37782..39056)
                     /locus_tag="ACGI6B_000863"
     CDS             complement(37782..39056)
                     /locus_tag="ACGI6B_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9750328.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39340..40497)
                     /gene="ftsZ"
                     /locus_tag="ACGI6B_000864"
     CDS             complement(39340..40497)
                     /gene="ftsZ"
                     /locus_tag="ACGI6B_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFG9750329.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40687..41040)
                     /locus_tag="ACGI6B_000865"
     CDS             complement(40687..41040)
                     /locus_tag="ACGI6B_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HFG9750330.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41139..41813)
                     /locus_tag="ACGI6B_000866"
     CDS             complement(41139..41813)
                     /locus_tag="ACGI6B_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9750331.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(41818..42516)
                     /locus_tag="ACGI6B_000867"
     CDS             complement(41818..42516)
                     /locus_tag="ACGI6B_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9750332.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42539..43279)
                     /locus_tag="ACGI6B_000868"
     CDS             complement(42539..43279)
                     /locus_tag="ACGI6B_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HFG9750333.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43549..44775)
                     /gene="murG"
                     /locus_tag="ACGI6B_000869"
     CDS             complement(43549..44775)
                     /gene="murG"
                     /locus_tag="ACGI6B_000869"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFG9750334.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44793..45923)
                     /gene="spoVE"
                     /locus_tag="ACGI6B_000870"
     CDS             complement(44793..45923)
                     /gene="spoVE"
                     /locus_tag="ACGI6B_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HFG9750335.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(45975..47330)
                     /gene="murD"
                     /locus_tag="ACGI6B_000871"
     CDS             complement(45975..47330)
                     /gene="murD"
                     /locus_tag="ACGI6B_000871"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFG9750336.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47349..48317)
                     /gene="mraY"
                     /locus_tag="ACGI6B_000872"
     CDS             complement(47349..48317)
                     /gene="mraY"
                     /locus_tag="ACGI6B_000872"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFG9750337.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48358..49731)
                     /gene="murF"
                     /locus_tag="ACGI6B_000873"
     CDS             complement(48358..49731)
                     /gene="murF"
                     /locus_tag="ACGI6B_000873"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFG9750338.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49898..51877)
                     /locus_tag="ACGI6B_000874"
     CDS             complement(49898..51877)
                     /locus_tag="ACGI6B_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HFG9750339.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51889..52236)
                     /locus_tag="ACGI6B_000875"
     CDS             complement(51889..52236)
                     /locus_tag="ACGI6B_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750340.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52717..53652)
                     /gene="rsmH"
                     /locus_tag="ACGI6B_000876"
     CDS             complement(52717..53652)
                     /gene="rsmH"
                     /locus_tag="ACGI6B_000876"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFG9750341.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53682..54428)
                     /gene="lgt"
                     /locus_tag="ACGI6B_000877"
     CDS             complement(53682..54428)
                     /gene="lgt"
                     /locus_tag="ACGI6B_000877"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFG9750342.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54822..56108)
                     /locus_tag="ACGI6B_000878"
     CDS             complement(54822..56108)
                     /locus_tag="ACGI6B_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9750343.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56123..57388)
                     /locus_tag="ACGI6B_000879"
     CDS             complement(56123..57388)
                     /locus_tag="ACGI6B_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9750344.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            57704..58801
                     /gene="ychF"
                     /locus_tag="ACGI6B_000880"
     CDS             57704..58801
                     /gene="ychF"
                     /locus_tag="ACGI6B_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFG9750345.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        18506 a        10670 c         6349 g        23445 t
ORIGIN      
        1 ttatagaatt ttcacaataa aaagtcattt tacttccgta tttaatgcaa aagtagaaat
       61 taaacttaat ataaaaaata tataacaaat tttttttata tttctagaag aaaagtatac
      121 tcttttttgt ttaataatgc taaaattgat ttgtattgaa ttttgattag gaggaaatta
      181 ttatgaaagc tcaaagatta aatgctgtac ttgaacaaat gaaaaaagat gatatttctc
      241 aaatgttagt aagtgaccct acttctatat tttatcttac aggagtacta atccatccag
      301 gcgaaagact attagcatta tacttaaatt taaatggcaa taataaatta tttataaatg
      361 aattattccc agtttcagaa gatttaggtg ttgaaatggt ttggtttaat gatactcaaa
      421 atccagtaga aataataact gaacatatag acaaaaatgc tactatgggt gttgataaaa
      481 actggccagc tagattctta ttaaacttaa tagaattagg tggaggaagt aagtttgtaa
      541 attcatctta tataatagat actttaagaa tgtgtaaaga tgaagaagaa aaagagttaa
      601 tgagaatagc ttcaaaatta aatgacaagg ctatggaaca acttaaagct actgtttctg
      661 gtgaacttac agaaaaacaa cttgttggta aattatctaa aatatatgaa gacctaggta
      721 ctgatggatt ttcatttgac cctattatag gttttggacc taatggagct aatccacatg
      781 gtgaacctgg aaatgcactt gtgaaaccag gagatgcaat aatcttagat ataggttgta
      841 taaaagataa ctattgtgct gatatgacaa gaactgtatt ctataaagaa atacctgaaa
      901 aaggtagaga aatatttgaa atagttcttg aagctaataa gagagctgaa gctattgtaa
      961 aaccaggtgt tagattctgt gatatagatg ctgctgctcg tgactacata actgaaaagg
     1021 gatatggaca gtacttcact catagaactg gtcactctat aggacttgaa gttcatgata
     1081 aaggagatgt atcttctata aatacagata ctgtacaacc aggaatgata ttctctatag
     1141 agccaggtat atatttacca ggtgagttcg gagttagaat agaagattta gtacttgtta
     1201 ctgaagatgg ttgtgagata ttaaacaaac atgacaaaga aatatgtgta gttggttaat
     1261 tctaaaatca atttgataaa attaaaaagc catataaaaa tgttttaaac atttttatat
     1321 ggctttaatt tctcaatcta tgatttattt caatccatta tttatattgc agttattaag
     1381 attgcaccct ttgaagtata tgtccccatc actgaaccaa tttcacagat tacaacatct
     1441 ttgtatttta catccttttc cataacttcc cttacctttt gagccttttc tgggcaatta
     1501 gcatgtccta taaataaaac cttttcttct ttatctttaa tttttgactc tactaaagta
     1561 atcagctttt tcaaactgtt tccttcacct cttgccttat ctataggagt tacaacgcca
     1621 tctgcaactt gtattatcgc cttaaaattt aaggtattta taatttttcc tgctattgga
     1681 tttattctac cacccttaat agcattttct aaagtttcaa gtgttccaaa aaatggagga
     1741 ttatctttat atttatttat tattgaaaca atctcttcaa tttcttttcc ttcttttatt
     1801 aatattgctg cttttaacac taattgtcct attccaattg acccactaag tgaatctata
     1861 acctcaactt tcttgttatt tccttccttt tcaaacatat cttttgctaa aagtgcacta
     1921 ttataagtcc cagataatcc tgaacttata ttaatcacta atacactttc ttcctgacaa
     1981 tggtatgctt ctaaaaattt atcaggagaa ggacatgatg tttttggtag tactttactc
     2041 tcactcattt ttctatagaa agtttcatta tctatttcct ttccagatat ataactttct
     2101 tctccaaaag atacatttaa accaacaact tctatgttgt atttttctat tatatcatca
     2161 ggtaagtcac atgcactgtc cactatcaat tttattttat ccatattaac ctcactataa
     2221 gatattattt tatttattca gtcggtcgta cgaccgattt aattattatt ttatatattg
     2281 aatgaaaaaa agtcaataga tatagattct cgctttattg acaataaata tccataagct
     2341 tataatgtta ttaatttgtt ataaatatac atggaaaggt aggttttatg aaaacaaata
     2401 atatgaaaca ggctatttta tcatctgcaa ccaatttaat agccaaaaat ggagttcaaa
     2461 atactagcct tgctgatatt gcaaaagatg taaatataag taaaggtact ttgtattatc
     2521 actacgcatc taaagatgat attatatatg atattgcgga tactcattta gaagttataa
     2581 ctagtgctat actaaactgt gtaaaaaatg tcaaatcaaa aaattctcaa atagaaatgg
     2641 ttaacttaat cttagagaaa atatctacta tagaaagtcg tggtagggta catatgtatc
     2701 ttatttgtga ggcaattact tcaaataatg atttaaagga aagaataaga ttaaaatata
     2761 ttgaatggag aactactctt aaatctgaaa ttgttgaatc actagaaaaa tataatagta
     2821 gcgaaaatga acaggatgct gaatcatttt cttttttact catgtcaata gttgatggtt
     2881 tagttgtcca aagtttatta aaaacagaaa aaatacctta tgaaaatata gcttcttttt
     2941 tagttaatca ttggatttaa acttaatact tagataatta ataaaagtga actatttaag
     3001 ttatctactt taacagttca ctttcttatt tattctcaat aatttttact taaaacattc
     3061 aatttttatt taaaccttca tggaatcaca aaataaaaat ctcttataaa tttaaaatgt
     3121 ttttatttat gttcatgtgt atgcccatct gaatgttcat gaccatgtgt atgtgtatgc
     3181 tcatggctat gctcatgagt acctgaaaaa ccatcatcat gattatggtc gtgacctaca
     3241 ccatttacat gctcatgttc atgcccatga ctgtgagaat gttgatgtga atgttcatgg
     3301 ttatctcctc cacagcatcc ttctcctcca gtcccacaac acccacttgt atgtggatta
     3361 tgatgatttc ttactacaaa tctctctctt aacataattc ttatacctcc tagtatatta
     3421 ttgagtactt catgtacccc attaactaca gtatacatct aactaggtat aatctcaata
     3481 catatggcat tttttaacat atataattaa catatataat cttaaagaaa tttatcctta
     3541 aaacctccac cttttacttc ttcaacgtta ttgacagaaa taaaagcttc actatccata
     3601 tcataaatta aatctttaaa tttacctatt tcatttgcag atattatgca ataaatcatc
     3661 tttttcttat tttgtgtata tgcaccttct gcctccaaaa aagtgactcc tctacccatt
     3721 ttattcatta tcactttgga tatttcatca tatttatttg atattaaaag tatagatttt
     3781 tttctatcaa agcaatcttt tcctatatcc atagtaaaag aaattatata cattgtaatt
     3841 aaagtgtaca tcaccaattt agctccaaat aaaaaagctc ctgcacatac tacaaaaaaa
     3901 ttaataaata aaaatgtatt tttcataggt atatcatact ttcttttcat tatggctgct
     3961 attatatcaa gtcctccagc agtagctctt gctttaaaag ttaaccccat acctatacca
     4021 gttaatgcac caccataaat acactgtagc atagtatcat ctattgggaa atatttagca
     4081 atatcttgtg ttagccccag tgctagagaa aatagtaaca tattgacaaa acttattagc
     4141 aaaaatttat tatcaaaata ctttctagct ataataaata ttggtatgtt catcaagaaa
     4201 cttgatattc cctgattaat accaaataat ttgtataata taacacaaat tccagctact
     4261 cctccactta ataacttggc tggtgctaga tataaattta ttcctaaggt tgatagaatc
     4321 atacccaata taataagaca atattgtaaa aatatattat tatcaatact tagcttttta
     4381 ctttcaccat acatcaaaac gcaccccttt gcataataaa taaaccaccc tgcataacac
     4441 acgataaatt tacaaataaa tttatcagaa gtagtttcct aaaaatatta ttcatcctca
     4501 cttaaacgtt tattcattaa tattctatca catgacataa ataatttata tgatttatat
     4561 agatttattt attgtaaatt ttggaatatt aaaacatttt attgaataga taattatatt
     4621 acataaaaaa tccctataga aatctataaa ctaatttcta tagggatttt ggttaactaa
     4681 ctattatttt tattttttta taacttttat acctgtcata tggtcattta agttttgttc
     4741 ctctaaagat ttatcatcta ctctgtttat ttctaaagct aaagtttcag acttgatata
     4801 ttcattaaaa tggtcaacag ccttagcaat ttcatcatca ccacagtatt ctattactat
     4861 attatctaat acttcaaagt tattagattt tcttaattgc tgtactttag atataaattc
     4921 tctagcataa ccttcattaa ttaactcttc agttagctta gtatctagta taacaaaaag
     4981 attgttttca acagatacat taaatccttc cttagcagtt atacctatta atactaaatc
     5041 cttattaaat tcaaaagctt caccatctaa atcaactgtt aaagtctcac ctttttcaag
     5101 tttattagca gtttcatgag catttaattt acttaatgct ccaacgaata aattcatctt
     5161 agaacctaat aatggccctg cttccttaaa gtttggtttt aaactaaagt tcatatactc
     5221 atctaaatcc ttagcaaata ttacctcttt aacattaagc tcttctttta ttaaatcaac
     5281 tacatcactt attgtatctt caaacttacc atctactaac actttttgta atggttgacg
     5341 aacttttatt ctttctactt ctcttgaagc tctacctaaa gtaactaagt ttttagctaa
     5401 atccattttt tcttctaatt tagtatctac taactcttca ttacatttag gatattcagc
     5461 taaatgaaca gatacttctc ctgttaagtt tctatacatt tcttctgaca tatatggtgc
     5521 aaatggagct atagctctac aaagaccatg taacaattca taagttgtat tgtacactgc
     5581 ttttttatct tctgttaatt cagttgccca gaatcttctt cttgaacgtc ttatatacca
     5641 gttagataaa tcatcattta taaaatcttg tatcattctt aaagttttat ttaactcaaa
     5701 tatagctaaa ttttcttcta catctttcat taaattattg aacttagata atatccatct
     5761 atcaagttct ggtctatctt tatattctac aaaaaattca gttggattta tatcatctgt
     5821 gtttgcatat aaagtaaaga agttatatac attcttcata gtacctataa atttactttg
     5881 gatttctttt aatccatcca tatcaaattt agttggagtc catggtggag aaacatatag
     5941 taaataccat cttaatgcat ctgctccata ttggtcaaat aattccattg gatttactgt
     6001 atttcctctt gatttactca ttttcttacc atctttatca agtacaagat ctggaactaa
     6061 aacactctta tatggtgctt ttcctaatac aaatgttgat actgcaagta atgagtagaa
     6121 ccaacctcta gtctggtcta taccttctga tatatagtca gctgggaaca attggtcaaa
     6181 gttttcctta ttttcaaatg gatagtggtg ttgagcaaat ggcatagccc cactatcaaa
     6241 ccaacagtct ataacctctg ttactcttgt cattggtttt ccacattttt cacattttaa
     6301 gtgtatatca tctacatatg gtctatgaag ttcaactgtt tctggattta catcttctat
     6361 agctttttca gctaattcag ctcttgaacc aactgaatct gtatgaccac attcacatct
     6421 ccatatagga agtggagttc cccaatatct agttcttgat attgcccagt catttagatt
     6481 ttctaaccag tttccaaatc tcttctctcc tacaaaactt gggaaccatt ttacaccatt
     6541 attattttct attaatttat ctttaagttt agtcatctct atataccagc ttggttttgc
     6601 atagtatact aatggagttt gacatctcca gcagtgtgga taattatgaa ctactttttc
     6661 tttgctatat aatttatttt cagaagctag ccattttatt atttcaacat ctagcccatc
     6721 ttccattaca aatctacctt cccaaggagt agatgtaaat ttaccagttt catctactgg
     6781 ttgaataact ggtaaatcat atcttttacc tgtgttgtag tcatcttcac caaatgctgg
     6841 tgctgtatga actataccag ttccatcttc agtagttaca taatctccta ctgtaataaa
     6901 aaatgctttt ttgtcaactt ctacaaaagg cattaattgt tcatattcta aaccttctag
     6961 gtctttacct ttcatttctt ctaaaacttc ataatcttca cctagtactt tatcagctaa
     7021 aggtttagat acataatata cctcatcacc ttttttaact tttaaataat ctacatcagc
     7081 atttactgtt aatgaaacgt ttgatggtaa agtccatgga gttgttgtcc atgctaagaa
     7141 gtattcatca gcatcttttt tcttaaattt agctataact gtattagttt ttacttcttt
     7201 atatccttgt gcaacctcat gactagcaag accagttcca catcttgggc aataaggaag
     7261 tattttatgt ccttcatata tatatccttc tttgttgaat ttgtttagta tccaccatac
     7321 agactctata tagttattat ctaaagttat atatggatta tctaaatcaa cttcataagc
     7381 cattctctca gtcattattc tccattgttt ttcaaatgag aaaactgact ctctacattt
     7441 ttggttaaat tcagctattc catattcttc tatttgttgc ttgctagtaa gacctaactc
     7501 tttctctact tgtatttcaa ctggtaatcc atgtgtatcc caaccagctt ttctctttac
     7561 ttgataacca gacattgttt tatatctaca aactgaatct ttaagcgttc tagataaaac
     7621 atggtgtact ccaggatttc cattagctgt tggaggtcct tcatagaata cgaaacttgg
     7681 gtcatctttc cctttttcta aagttttttc aagtatattt atgtctttcc aataatcaaa
     7741 aacttgtgct tctgcttgtt ttacagaact gtctaccaat ggcttaaatt tcgccatgta
     7801 aatcaccctt tcctactaaa tattataatt ccaaaatttt ttattctttc acataaattt
     7861 aaatactaaa aaactcgccc cctttaaacc aaagggacga gttataaatc gcgttaccac
     7921 cctaattcta tatgtaaact aaatactagc tacatataac actctaatag tataacggta
     7981 ctaaccggca cagcttagta gaatttaatc gttcagcctg cagctcaaga gtgattttta
     8041 tctttataaa ttcattggtt ctcagcatac ccaattctct gtaaaattat ctaaagataa
     8101 atgtctctat caacgctttt aaaaatttat ctgtgtttat atgaaattct ataacaaata
     8161 atttgaatag tcaatatatt tttgaaaaat ataataattt ctatttttct tacattttta
     8221 tcctattttt aatctttgtt atagatttct caaactcaca caattattct acataaaaaa
     8281 taaacaaatt acaagcttat tctaaagttg tagcagcttc atcattacta taattataca
     8341 ctgttgttcc ttcaaatgca tttacacatt tttcatctac atcataaaaa atctcatcaa
     8401 cacttcttat ttcatcttct aacagtgatt taaatttatt tctaaatatc ttaaattctt
     8461 tgactaaagc atcatactct tttcttatct ctataacacg gttattagct tgttctataa
     8521 tttgtctagc ttttaaatca gcttcttcaa caattatctt agccttttta ttagccgcgc
     8581 tacaagtgtc ttctgctgca ctttgagcag ttattaaagt agcttttagt gtttcttcaa
     8641 tattttcata cttgtttact tggtcttgat ataaccctaa tttttcttta agagctgtat
     8701 tttccctaca tagagattca taatcttcct tgatgatatc taaaaattca tcaacctctt
     8761 catctctata ccctcttaaa ccttttttaa actctttatt ttctatctca attggagtta
     8821 gcattacatt tcctcccagc tataatttaa attagtcctt atacaattaa tcttgcttga
     8881 acttttattt ttcctttttt agtaatatct cctatttgtg ttaccttagc tcttcctttg
     8941 cctctaatag aaatcaaatc atcgtttttt actattttag aagttgaagt tatcttttca
     9001 taattgacat ggactttttc tccatttatg tattttgcac tatcttgtct agatatattg
     9061 taaataccac ttatcacaca atctaaccta tctgaagata ctgtaaatga tacttcttta
     9121 tattttgaag aactattaac aatattttct ctggaaattt cctttacaat tacattattt
     9181 cttgaaacct tttctaagtt tacaattata aaatcgcaaa tatcactact tacaactact
     9241 tgacaaaagc tatcatgtat aattatatct ccaatttttt ctctttttat acctagaccc
     9301 agtattgacc ctaagtaatc tttatgagat atatttttaa atttgaaatt cccttctatt
     9361 tgtaaaaatc taagagtatc ttctatatct tcatattcca tgtaaaatgg ataaataaat
     9421 acagtgcttc tttcagcttg ttcataacct ccatccacac tgtattttat ttcatttgta
     9481 gaatttaata ttgctacagc atttttcact tcatatggat taagaaactc cgtagactta
     9541 acatcatagt tttttataca actattagcc ttgtctatca ctttaaacat tttatttttc
     9601 aagtctatat cctttatgtg atttgttagt tttaatttat ccatcctatc accttagaat
     9661 ataaccaata ctaattgtct taggaacatc aataatacta ttgcaattaa aggagaaaaa
     9721 tctataggtc catttgtata tttatacatt atagacctta ttggcccttc tattggctct
     9781 gtaagatttt ccaaaatatc aaaaatttta ctttgtcttc catttggaaa ccaagtcatc
     9841 aagcttttta taactatcat ccatgctatt atatctaaaa gataataaag tgcaattctt
     9901 attgttccca tatattaacc gcctttttac tatttatttt gccaagggaa gaatgcttta
     9961 ctttctaatt cttttttcat atttgcatct atatccacat tattaggagc tagtataaat
    10021 attgatttag aaaccttttg tatacttcca tctaagacat aaactgcccc attcataaag
    10081 ttaaatatag atttacgaac ttcagaatta gtcaatcctt ccaaattcac tataactgcc
    10141 cttctttgtt ttagatggtc tgctatagtt gcagcttcat cataaacctt aggttcaact
    10201 attacaactt tcatttggct agttgtatgt aaatttacaa ttttatttgc ttttgcagta
    10261 ctaaagccac tgttcatttc ttcttcttga actatatcgt ccatgccact ttcatattca
    10321 tcttcaatgt aatcttcttc ttcatcaact atccaattct taaatttaga tattattcca
    10381 tttgacatta ttaacttcct ccttaaaatt tattcaaatt tgagtaatta ttttttattg
    10441 tagtttcttt ctccaaagat tgatgttcca actctaacaa tggtagcccc ttcctctata
    10501 gcaatttcat aatcgtgact cattcccatt gacaaaacat ccatccctac attagggatg
    10561 tttagttctt ttatttgttt tgataaatct ttaagacctt taaatacacc tcttatctca
    10621 tcttcatctt ctataaaagg tgccattgtc attagaccct ttatttttat atttttatat
    10681 ttttctgaca cttctttgac aaagtctaca acttcttctc tatcaagtcc atgcttagac
    10741 tcttctttag atatattcac ttgtactaag caatctattt ctctgtttat ttgttctgca
    10801 cgtttttgaa tttcttcgca taatgcaatt ctatctaaag agtgtatcat acaaacttta
    10861 tcgattatgt acttaacttt attggtttgc aaagttccta tcaggtgcca gtttaattta
    10921 tcacctataa tatcatactt tctagctaat tcttggggtt tattttcacc aacagaagtg
    10981 attccttccc tcattgcctc attaactaag tctacatcaa cagtctttgt gactgccaaa
    11041 agggtaatct ctttgctgtc tctatctact ttttttgcag ctttatcaat tttgtttctt
    11101 atctcagaca aattatcttt aatagaaccc attattatca cctactttat ccttatattt
    11161 gtgtttatac tgttaggtct taaaattatt ttgtcatata gattaacact tgtaactcca
    11221 tttatgtcat ttttataata atccacaaat acagaagatt cattctcata agttattcct
    11281 tctaatatga caaattttac actttgtgat tgttcatcaa ttacatatac accacgttta
    11341 ttgtcttttg tttttataga agttttaggt atttttaaac attccatctg attatatatt
    11401 atatcaaatt cttcgacact tgtatcctga tttgcaacaa tttggttact tatttttaat
    11461 tttaccatta ttttattatt attttcatat attttgtaaa cttttgcatc tagtttttgc
    11521 tcatgatatt caattattac attttgattt ttttcaaagt tattagaatc ttctttactg
    11581 atgtatgtag atagataatt actttggtca ttggcatctt tttctatagt tccttttaca
    11641 tgaatctttg catcaacttt ttcatttttt aacaccaacg tatctacatt tttgctaatt
    11701 atcattacta atatattaaa tactaaatat ataagaatac caagtatcac taatctggta
    11761 tattttacct ttttcaaaga ctctccccct ataaaattac tttcattata agtacttctt
    11821 ctaaaaaatg tttattcctt ttttataaga taatatttta cgttctaata attatttttt
    11881 ccatattaaa tttatttttt atcaatcttc ctatacaaga ctgcattatt tatatataat
    11941 tgtattataa agtataagtt taatttacat tttatacgtt aagcttactt tatatatttt
    12001 tatagttatt tttattttta gatgcaatat aaaaccaatt aagatttaaa ataacttttg
    12061 ttatgttgac atttatttag gtaatcattt atgaattagg ataacgttat cacaacaaaa
    12121 gtttatgtcc taaaaaaaat acaaggagga ttttcttatg tcagaaattt tattaggaac
    12181 aggtttactt ttattggttc tatgttgttt cacaattttt agttacaagg ccccatatgg
    12241 tatgaaggct atgggagctc tagcaaacgc tgcatgtgct actttcttag tagaggcatt
    12301 caacctttct ttacttggtg atgttttagg tatggaattt tttagaagtg ttggagctgc
    12361 taatggtagt cttggtggag ttgctgctgc tatacttgtg cctttagcct taggtgtatc
    12421 tccagtttat gcagttttaa taggattatc ttgtttagat ttttctatat tatctggatt
    12481 cgttgctgga tactttgttt ctttcttaat taagtttata gagaaaaaag tgccacctgg
    12541 agtggattta gtggttgtaa ttcttctatg tgcaccacta tctcgtctta tagcatcaaa
    12601 tgtagaccca ttagttaata gtacactact acaaattggg caagttttga tagaagctac
    12661 caacattagc cctataataa tgggaattat actaggaggt ctaataacag tagttggaac
    12721 gtctccactt agttcaatgg ctcttacatc tataattggt cttactggtc ttcctatggc
    12781 aataggagca ttaggtgctt ttggttcagc ttttatgaac tcaacactat ttgcaaaatt
    12841 gaaattaggt tctaaaaaag atattatatc tgttgctatt gaaccattaa cccaatcaga
    12901 cataatttca gctaatccaa tacctatata ttctactaat ttctttggag gagccttttc
    12961 tggtatcatt gtagccttga tgggtcttgt aaacatgaca ccaggtacag ctacacctgt
    13021 ggctggtttt gctataatgt ttgcttataa tccagttcaa caagttctta taacagctgg
    13081 tttatgtgct ttattcaatg taattgcagg atttttaggt tcaaaaatat tcagtacctt
    13141 taaagttctt actgctggtg aaatacgtgg tactgatgta gaagaagaaa ttgcttaaaa
    13201 aacaataaga tttataaatt tatatctcaa gtagtagagt tttatattgt aggcaatttt
    13261 attaattgca aacgttttat tttttattta tgtttatgat tattatgtgt gttgaatatt
    13321 tagataaatt tttatttatt atttttattt agatttttgt ttagtttttt atttggttga
    13381 tatattagga ataaaaatta gtatagttta ttactagtta gatggcataa ctttttagat
    13441 acataaaaaa ttttatatta attagatatt attaaaataa aagcgataca tgaattttta
    13501 aatgtccatg tatcgcttta tttttgccat aatcttaata actgattttt aaagtttatt
    13561 atcttaacaa agttcattat cttatataat atttaaatgc ataggcttct ttaatttaac
    13621 catctaaata tttaattaaa tattattata tgtaatataa ctaattttta aatgaatgta
    13681 taggagctgg aatccttcct cctctttgag taaataattc agatgaattt gtatttatag
    13741 gcattactgg tgctcgtcct aaaagtcctc caaattctac catatcacct acatcacatc
    13801 ctggggctgg tataattcta acggctgtag tcttattatt aatcatacct attgctgcct
    13861 catcagctat catggctcca agcgtacctg ctgtagtatt tcctggcaca gctatcatat
    13921 caagacctac agaacaaata gcagtcattg cttcaagctt atctatactt aaagcacctt
    13981 taataacagc atcaatcata cctgcatctt cagatactgg tataaacgct ccactaagtc
    14041 ctccaacatg tgaacatgcc attactccac cttttttaac agcatcattt aaaagtgcta
    14101 atgctgctgt agtcccatga gtaccaacaa cttcaagacc catttcctct aatatatttg
    14161 ctactgaatc accaatagca ggtgtaggag ctagtgataa gtctattatc ccaaatggaa
    14221 catccaatct actagaagct tcctttgcca caagttgtcc aactcttgta attttaaatg
    14281 cagtcttttt aactgtttct gaaacaacat caaaaggctc tccctttact ttctctaaag
    14341 ctctcttaac aacaccaggg ccacttacac caacatttat aattctatca gcctcaccaa
    14401 caccatggaa agctcctgcc ataaatggat tatcttctac tgcattacaa aatactacta
    14461 attttgcaca accaaaacct tttgcatctt ttgttaattc tgcagtttct tttatgattt
    14521 ctcccatttc tttcactgca tccatattta taccacatct tgtagaacct acatttactg
    14581 aagcacatac tttattagta cttgctagag cttttggaat tgacttaatt agtatcttat
    14641 caccttttgt atatcccttt tgaactaatg ctgagaatcc accaataaaa tctataccta
    14701 aatcctctgc agccttatcc aatgtctgtg caaactttac atagtcattt tcatctgttg
    14761 cacctgcaat aatggaaatt ggagttactg ataccctttt atttacaatt gggataccaa
    14821 attcacgctc tatatctttt ccaaccttca ccaaatcttt agcagaactc ataatcttat
    14881 tgtatatttt ttctctagct ttatctccat cagggtctat acaatctaat aaagaaatcc
    14941 ccatagttat agttcttata tccaactgtt cttcatctat catctttatt gtttcaagta
    15001 tatttcctac attcactatt atcacctccc cttactgtct actacagatt atgcattgaa
    15061 ttaaatattt cttctcgctg aatttttaca tccactgata atttcttacc tctttcaacc
    15121 aagtcctcaa ccgttttctc aaaagaattt aatgatttct ctaaatcaac tatcataatc
    15181 atagtaaaat aatcctgtaa tattttttgt gatatatcta ctatgttgat attttgcttg
    15241 tataattcat cactaattcc tgccactata cctggtttat cctttccaat tactgttaaa
    15301 attgctttca taataatatc ctcccttatt tttttataaa tcttatattt ttacaaattg
    15361 aactatgttt taacatatat ttcatttatt tgttagtaaa ccacttttta tgtatatatt
    15421 taatatattt taatacctat acattcattt gtcaattttt tttcaatatt ctattatttt
    15481 tatactattt agaaaattca tatattatat atgttttctc aacaataaat acagttttca
    15541 taataatgaa aggtattact tagcaatcgc atgattttct aaataatacc tttctaaaaa
    15601 ttattttttt aattttatga atttaattat actttttcta gtatcaggat ttacaattaa
    15661 tatagaacca atcataattg gtgttagtat acaaaataaa aaaataatat ctttttttag
    15721 attatattct ttagattcta atttataatt attatttatt attccatcat aatcacttat
    15781 atttgactta tttttatttg tatcttgatt tttgttagaa atactttccc tataaggaaa
    15841 agaatgttca ttggtaccaa atactaattt tgagcttaaa tcataattat catcattttc
    15901 tgtaattgaa tttttattgt aaaatccttt ttctttatca gaattatttt ctttattacc
    15961 gtctttaggt atatcatgag gatttttatt ttcattgcta tcagtacttg attctacatc
    16021 atctttagga gtatttggtt gtttctcatc agaattatca ttttcattat tattattttc
    16081 attattattg ttttgattgt tattactatc attattttca tttccattat tcccatcttg
    16141 attgttatca tcctcatctc tatccgggtc gtctctacct cctcctccac ggtcaccatc
    16201 aatatcatca ttttcccgtt catcatctgg tgtctcttgg ttgtctccgc ctcccattat
    16261 aaatcctggt ttaaaaacta tatttgaata cccttctttt aatcctccta ccacttttac
    16321 tctaaaacaa cgcaacttaa aatcttcaaa aaaaagtata gcttgatctc taatatcctc
    16381 ctgttcttca gatatccaat tgattccatc gcttgaatat tcaacaaaaa ccttacttgc
    16441 actatacggc atatcataaa ataaaacagc tacatatcca ttttttgtat tttgaaattc
    16501 cagttccaaa ttgtctattg gttttttctc tataacagat acattcaaaa taggaactac
    16561 atcatcattt attataattt tttctttatt taacatttct gttttaaact ttcctgttat
    16621 attaaaattt tctccctttt ccaacttttc ataataatca gtaaaatccc attctacatc
    16681 tatatcaagt cgattaccac tagatgtttc taataccata tatttgggaa attttatatt
    16741 ttctatttta tcatctttac ttacttttat ttcatcacta aatacaccaa ttcccctatg
    16801 acaacatttc acaatatcat agccagaatc ttcagctata gagggtataa gctcattata
    16861 atcatcaatt atataaactt tcttactttc tgatataatt gattgtgcca acttattatt
    16921 atcttttagt aacccacttt cattgttgct actatatgct tttattgaag tttcttctat
    16981 ctcaacatca cctttagaat aaacacctat agcatgttct ccattcactt caatatcctt
    17041 atttagtatt ctaatatcat tttcagtata aattcctatt gcatctttcc catttgcctt
    17101 tattttttca acagattcgt aggatgattt aatatgtaag tctcctcctt cttcaacgta
    17161 taaggctgtc ccatcagtag caattatgtt gtttatacca atacttatac ttccaccatt
    17221 ttctatatgt attactcctt cttttcctcc ttcacctatt atattcaatt cattatcatc
    17281 aataataaat ctcccatgat tttttatcaa tattttatat ttgttggtat ctattgtaaa
    17341 attactgtca taaggaatat aaaatctaaa ctcttcatct attaccatat cacaagaaag
    17401 agtataaact atttcttctt tattattttt attttcttga acccaccttt caaaatcttc
    17461 aagacaattt tcctcttgtc caaaaacttt atgtggaaaa cttataaaac acaaaactat
    17521 tatactcata aaaataaata tctcatactt tttcatctta cactcctaga aattttttag
    17581 ctttgaaaca atctgtcgaa acatacactt cacccactat aatttttcca caattatcaa
    17641 ttattgtcaa tttataaaag tatctttatt aattgttata aataaaaagc tacttactaa
    17701 agtaaacaga cgtttcactt taataagtag ctatattttt ataacaaaat agttataata
    17761 ttagaacttc cttcatttac aattttttgt aaagtctttt gcattttaac tcttatctct
    17821 tctggcatag tatataattt actttgtaat tgctctttta ctaagtcatg tagtgattta
    17881 ccaaacatat ttgattccca taaatctgct ggttgctctt caaatacttc cataagatat
    17941 tttatcatct cttcaccttg attttgattt ccaactattg gagaaacctc tgtagatata
    18001 tctgctttta taatatgaag tgatggtgca ttggctttta acttgatacc atattgtttt
    18061 ccttgtttca ttatttctgg ttcttccaga ctaagttctt ctaaggaagg agctactact
    18121 ccatatcctt ttatcttggc atctattaaa gcactttcta ttttatcata ctcattttta
    18181 actttagata gtctagttat cagactaagc agttgataat ctccttcaat tttaaatcca
    18241 cttttttctt caaggacatt atagaataaa tcttgctttg tctgtaaatc aatatttata
    18301 acaccttctc ctagctctac attatcaaca cctgtatctt ccaaaaactc taactcagaa
    18361 aatccttgta taataccttc tatatccctt attttaccta tatctataat actttgtttt
    18421 aatgttgtta ttatgctact ttttatccaa tgatttcttt caagaccttc aacccatttt
    18481 ggtaagttga ttcttatttc tgtaagtgga aaatcatata atacagcttc cataacctcc
    18541 tctatatctt cttcttccat ttcaaccaca ttcattggaa gaactggaac ttcatacttt
    18601 tcttccaatt cacttctaag catactcgtt tcctcacttt ttggacttaa cgtatttaaa
    18661 accactgcaa atggtttttt aagatttttc aattcttgta taactctctc ttctggttct
    18721 acatagcttt ttctgtcaat tccagtaact gaaccatctg tcaagacaac tatccctatt
    18781 gtagaatggt cttttataac ttttttagtt cctatctctg ctgccttttc aaatgtcata
    18841 gcttcttgtg accaaggagt ggatactaat ctttgttttc caccttcctc atgccctaaa
    18901 gctccttcaa ctatgtatcc aacacaatct accattctta cttttaaaga tactgtgtct
    18961 tttattttta tttctacacc atctgctggt acaaattttg gttctactgt cattatcgtt
    19021 ttacctgaac cactttgggg tatctcatct cttgttctat cctttttaaa ttcattgtct
    19081 atattaggta taaccaactt ttccataaat tttcttataa aagttgattt tcctgttctt
    19141 acaggtccaa ctactccaat atatatatcc ccctgagtcc ttttggatat atcttcgtat
    19201 atgttattat tcatattatt cctcctatac atctaatctt attaaattat atttagagtt
    19261 aagggttaat attcttaaaa tagaaaaata aagaccagca atattaattt aatttatata
    19321 tagaaatttg taaatttcaa tgtaaattaa attaatattg ctggtccaaa tagtagtgta
    19381 ttatattaac tgactttagt agtattttca tttgaattat tattcatctc aataagttct
    19441 ttacatcttt cttttgtcat gttcatccat ataaatgtaa acaccaatcc aattatggct
    19501 actatagtaa gaactatgaa gtagtaccta tatccagtta cacccggaaa tgcatccaat
    19561 atcttccctg ctgctaaagg acaaagtact tctggaagat atcctaacgt agcaactatt
    19621 cctatagcag ttcctgatat tgataatgaa taattacctt cttcaagtaa cgaatagtgt
    19681 aaaccttgaa ttgcatacat agatacatat attgcaacac atccaatcaa tagtatataa
    19741 atcatagatg attttgttgg tgtaaatatt acacctaaaa gacctgcaat cataagtata
    19801 aatccataag atataatctt tgatgaacca actttatctc caagtaatcc agaagcagct
    19861 gatgctactg gtctacaata ctgagctaat attgagattg cagatgcaaa caccacagta
    19921 gctccaaatg cttcagttga ataaggcgta aaataataaa atgacatatt agctgaatat
    19981 gaacaaaaca ttattattgt tattatccat gtatgaggca tttttattac tgcaaaaaag
    20041 gcttctttat caaattttgc tgatacctct ttttcattat cctttaattt gaaaaatact
    20101 aatactccaa ctattacatt tattgctgag tataatacta taatagctgt aagaccagat
    20161 tcatcactta cttttgatga aatataccca aatagtatca atactagaga tagatgaatt
    20221 gcattggtaa ttccacgacc actttccata aatccaaaag cttttccttg ttcattttca
    20281 gatgctaaca ttctaacagc ttttacaaga gctggccaaa aagtaagtat tgttgttatt
    20341 ccccataagg catggattaa aaacacaaca atatatggag gataaagtgt taatacaaga
    20401 ccagatattc ctgtaataat taaagaacct gaaagcaatt ttctggctga aatacggtct
    20461 gctaaaaaac caccaaaaag gtatgatacc attgcaagta gaccataaat gcttcctaag
    20521 cttcccattt gtgtatttgt aagattaaat gcttgtgcaa aagtatcata ataataattc
    20581 tttaaatatg gtaaagcata tataaatgct cctgctacaa agagtattat caagactttg
    20641 aaatttttcc aaatgcttgg tttatttact tcaactgaca tacttcctcc ttatgtaaat
    20701 acctgtaaat tttcataata attaattatt gcttatttta ttgtttattc aatatttagg
    20761 aatatttatt aatatattag gttaatctat caataattta tggtgagtta tgagctaaaa
    20821 gtaaactctt ttagctcacc ataataatta tatttttatt tatactaaca tccagagtta
    20881 ttagatatct ttttttcata gttcttccat agcattaaac ctgttattat aacaaaaaga
    20941 cctcctccta tcatttcaat ctcatagatt atattatctt ttaaagtctc cataactggt
    21001 gccgttgaga aaagtatgcc aactacagtt actacaagta ccattctggc aatattgata
    21061 caaactgaat cactttttgc aattgaatat ggtctttctt catttggtct tgtctttcta
    21121 agtctaatat aagatgtaaa aagcaagaca tatggaaata atgctgttaa tgctgtcata
    21181 gtaacaagaa cattgtatat attatctact gatggtaata agtttgttcc tacaagaagt
    21241 agactcacta ttatagcctg aactattact gcatttgatg gtatattgta tttatttgta
    21301 acagtcaatt gcttaggaaa tacaccttct cttacatttc caaaaagcat ctttactgga
    21361 cctgctatat aaagtattaa tgctcctagt actgataaag ttattccaaa tgctactatt
    21421 cttataaacc aacttcctat acctaaatct tgtgcaactt tagctaatgc atctagtatc
    21481 cctgtagatg ctgcaatttt atctggagga agtatcattg ttattgctac tgaacctaag
    21541 atatagattc ctcctattaa aacagccaca gttaatatag ctttaggaaa gtttttctta
    21601 gcattatcca tttcggttat gaaattagca gtcgtttcag ctcctgataa tgcaaatatt
    21661 atagcagata tagaaactaa tgaattggca tctatttttg gtattatatt ttggattgta
    21721 tatatcgatg ctgatggagc ttttttgaga ataactacag acataaatgc catcactata
    21781 agtaatatag caggaattgt agaacctaat gctcctgtat tcgtaaaaaa ttttccaaat
    21841 gccatcccac gagtacttac taaggacaac agccaaaata ttatgagtga aagtgctaaa
    21901 ataaacatct tattgtttga taaatctgga tttccaagca tatatgcaat atttatcgct
    21961 aaaaaagtca agaaagaaga ataccaaaat atctttgatg tccaatttag ccatgacacc
    22021 ataaatcccc acttctctcc ataagcttgt ttaacccatt ctccaagtcc accatctttt
    22081 ggatatgttg cagccaactc tgcacatata agtgatgcag gaacaaagaa tataaaagca
    22141 aacaataacc aaactggtat agaaccaaga cccaatccaa aactagaggc agtagattta
    22201 gctatccatc taatcccaaa tagtgctgat atattcataa atactaaatc ccaaaatcca
    22261 attttctttt tcactgtaat tcctccaatt tcaaatttat tcagactcat acacaatgta
    22321 ttgacttaaa tatcaagtat aagttcttca acacttcttg gataattagt tataattgta
    22381 gcaccattct tacctactat tgctgtatct gagtgtctaa atcctccaac tccaggtata
    22441 tatatccctg gctccataga aaataccatt ccttcttcta atattaattc attgtcaaat
    22501 cttaaatatg gctcttcatg ttctgataaa cctaaaccat gaccaattct atgatttgaa
    22561 tataactcta gaccatactt tgcaacaaca gctcttgctg cttcatccac cattcttgct
    22621 ggtataccag cttttattgt atctaatcca gcttgttgag cctctactgc aatcttaaac
    22681 acttcttttt gtctttctgt tggtttgcct ataataaaag ttctttcatt ttctgctcta
    22741 tagttttcat accaaacctg tctactatgt attactatat ctcctctttg aagtattcta
    22801 gtattggaat ctaaatgagg ttgtgctgtt ctatcaatcc ctgaacaagt ccagtttgca
    22861 aatcctatat atgtatcagg ataattttca gaaacatatt ttagtaaagc attatcccct
    22921 gcaacgtcaa actccaattc actcatccca actctaacat tttctaaaga cccttttatt
    22981 cctatatcag ataggtaacc tgctatcttc aaaaattcta tttcttttgc atcttttaca
    23041 tatctcattt caaatatttt acttccaaca tctttgatag caaatgactt atcagttata
    23101 tactctgaaa agcttgcaga tactatatct ttttctattc caacagttgt tcctttaggg
    23161 tatttattta aaattatatc taagtaatgt ttatgtgata ttccatgttc tttcatttct
    23221 ggtatttcat aatacacata tacattatca acttttgcaa cttctttagc atgtaattct
    23281 tctaatccag gtactattaa ttctatttta tcttgagtta ctactgttac aattggtcta
    23341 gaataagtta ttgctttaaa tccactaata taaaattgat tgtctggttc aaatattaaa
    23401 gcaatttcaa tgcttttttc attcatgtac tttctcaatc tttctagtct catattattt
    23461 gacataatgc acctctttct taatattcag ataatttatt caatacaaca aggatgaata
    23521 tattatttta aaattcttaa tatttttagt acttgtcgta tttgtatgta aattaatttt
    23581 atttgtgatt attttatcat aatcatgatt attttgtcaa tcatgatttt attttaaaaa
    23641 attttttata ttctagtgat tttaaattat ctaaaatata acgagtatac attgtatttt
    23701 gtaatattat tcttgattta tggtaagttt aatgataaaa tcacgattat ataaactata
    23761 ttgattattt gtaggtgaat aaaatgacaa ataaagaatt acaaaagaaa aggatattga
    23821 tgtatttcat agaagctgct caaaatatta tggaaaacga aggtatagaa aatattacac
    23881 ttcgtaaggt tgcagatatg gctggctata atagttcaac tctatataaa tactttaaaa
    23941 atttagacca tttaatatca tttgcatcta taaaatattt taaagattat aacttgaata
    24001 tttcaagatg tatagaaaat gtcaatgatg aatataagaa atatataatt atgtggaaat
    24061 tattttgtaa gcattctttt gataatgctc aagcttttta ccaggtattt tttaacttgt
    24121 ctagtgatga acttagctat ataacaaaac aatattatga tatgtttcca gaagatttag
    24181 gtattcatga tagtgacata tctttaatga ttacaggaga aagtataaag gaaagaaata
    24241 agatactatt aaacaatctt gtagatactg gatatatacc agctaaagat ttagacataa
    24301 tcaatgatat aattatttct ttttatcaaa atctattatt cttaaaaaag aacacaggaa
    24361 aaaattctga tgatgatgaa ttaactatga aaatgatttc ttctattgaa tacataataa
    24421 aacatgctaa ataaaattaa aatatggctg tctcaaaata gagataagcc ttttttattg
    24481 ttgaaataat aaaaatatat ttaatttcat ataaaaaaga gtgcctcgta aactaaaaag
    24541 ttcattttga gacaccccta aaataaattt attatttatt atacaatagt ttatatatca
    24601 tcacctaaaa ctacttcttc catttcatgc tttttacttc tcatcattag ttctattcca
    24661 acttctttag gatttgaacc tttctttatt acagaatata tagcattagt tataggcata
    24721 tctatatcta atttttcagc aacatcatgt gctacttcag tagcagttat gccttctaca
    24781 accatcttta cttcttttaa agtatcttct aaactcatcc ctttacctat gagtatacca
    24841 gctcttctat ttctactatg catacttgta caagtaacta ttaaatctcc aatacctgaa
    24901 agacctgcaa aggtagacat attagctccc atagcaattc caagtctgct catttcacta
    24961 atacctcttg tcataagggc agcttttgca ttatcaccat accctaatcc gtcacatatc
    25021 ccagctccaa atgctataat attttttaat gctcctccaa gttctactcc aactatgtct
    25081 ggattcgtat aaactctaag tttagggctc ataaataaat cttgtatcat ctgtgctatt
    25141 tttaaatctt tagaagcaac tactacggta gtaggaatat cccttgctac ttcttcagca
    25201 tgagatggcc cagatagtat tacatatgga ttttgtggta attcttcttc acagacttga
    25261 gataatctaa gcccagtccc tttttcaaga cctttagcta cattaacaat aacttgtcct
    25321 tcttttataa atggttttat tttttggcaa gtacttctta cagcctgtga tggaactgct
    25381 aaaactatta ccttacaatc ttttatagtt ttttctatat cagtacttat agttatatta
    25441 tttggaaata atactcctgg taaatagtct gtattttctt tagttctgtt tattttttta
    25501 gcttgttcct cttttcgagt ccacatgctc acatcattac ctttttttgc aagccctaaa
    25561 gctaatgcac ttccccagct tccagtacct aatacacata ctttctccat gtttaacacc
    25621 acctgagtat aatatattta ctattctttc ttttgaccaa tttttctctc tgtcccattt
    25681 aataatcgct ttatatttgc tctgtgatta tatattactg atgcagttaa gaacagagct
    25741 actattaaac cagctttatt ttttaccata atcataaata ttggtgaaca tcctattcct
    25801 acaacagaac ccaatgaaac atactttgtt atagctacca ccaatataaa tactgctaga
    25861 cacattagtg ttattactgg atttacagct agcattgccc ctagtgaagt tgcaacacct
    25921 ttgcctcctc taaatcctaa aaccgctggc caattatgac ctgctacaac acatataact
    25981 gccagatatc cagctaaaag agtatctatt ccaaccaatc ttgctgcaaa ttctgatatt
    26041 aaaacagcca ttacaccttt aagaacatca cctaaaaatg tcattgctcc tgctcttttt
    26101 ccaagtgtcc taagtacatt tgtcgaacct gcattaccag aaccttgagt ccttatatca
    26161 accccagcaa ttcgctttgc aactatgtat gaggtagata tatttcctaa aagatatgca
    26221 actacagcaa taattatata actaaatatc tccactgaat ttcccccctt tagttatatt
    26281 ttaagatatt tattttttat ttttttgtct atactcaaac tttatggaag ttccttcaaa
    26341 tccaaaattt tctcttatct tattctcaag gtatctttga tatgagaagt gagttaaatc
    26401 cttatcattt ataaacaatg taattttagg aggtctaatg tcagtttgag tcccatagta
    26461 aatttttaat cttctacctt tgtcagatgg tggttgattt aacattacag cttctccaat
    26521 tacttcattc agtgctgaag ttgatattct cttagaatgt tcatttgaca catattcaac
    26581 tgtatctagt atcttgttca ttctttgatt agtttttgca gaaacaaata cgattggagc
    26641 atacatcata aatggaaatt tttcttttat atcttttgtg taattgctca tagtcttatt
    26701 atctttctct attaaatccc atttattgat tacaaatatg catccttttc cttcatcatg
    26761 tgctatacct gcaacctttg tatcttgttc tgttacacct tctaaggcat ctattactat
    26821 caatactaca tctgctctat ctacagcact catagccctt attacactgt atttttctat
    26881 tgtctcatat attttacttt ttcttctaag ccctgctgta tctattagaa gaaatttctg
    26941 tccatttttt tcaaaataag tatcaattgc gtctctagta gttcctgcaa ttggacttac
    27001 aattactctt tcttctccaa gtatcttatt gagtatagaa ctttttcctg catttggttt
    27061 acctgttata gctactctta ttatatcttc ttcatactct gtatctaatc ctgctggaaa
    27121 attttcaact atctcatcta ataaatctcc aaatcccata ctattggctc ctgaaactgc
    27181 aaaaggcgtt ccaaatccca actcataaaa atcgtatata ttatcaaatt gactttgact
    27241 atcaatctta ttaaccacta agattactgg tttttttgtc tttctaagca attgtgcaat
    27301 ttctttatca gcagctgtta ttccagcttt accatcaact ataaaaagta ttacatgtga
    27361 catgtccatt gcaagcatgg cttgatttct catttgagat aatattatat cttcgctatc
    27421 aggttcaatt cctcctgtat ccactaatgt aaaatattta tctaaccatt ctacttcagc
    27481 aaatattcta tctcttgtaa ctccaggtgt attttcaact attgatatcc tttttcctgc
    27541 aaatttatta aatattgtag acttaccaac gtttggtctc ccaactacag ctacaactgg
    27601 cctacttata ctcattttat ttctccttcc atcttttgtt ttctatttta aaattttatc
    27661 cacaaaatct tttccttcat tcaaacaagg gataattttt attctcataa gttcttctaa
    27721 ttgatttaat tcaatattat ctaagaaaat ttcttcatct gcttttagca tacttcttgt
    27781 tatataaaga gcttctccta attctttatc ttcaagttgt tcttttaaat ccttagctgt
    27841 taaaagacct gatacagtta ttgtctctcc aaaaaacttg tttttgattt catatacatt
    27901 tacttgaaga tttttaaact tgtccatcat agcatctgcc atactctgta taaattcata
    27961 tgcagaatga ccagtagcta ttgatacttt cttctctctt tttagaatct tctcaggcaa
    28021 tatatttaga tactcttcaa tctctgtttt taacttacgt atcataccta caccatcttc
    28081 aaactgtaaa aaaccttcat attcttcata tccaggcaat tcactattag atagtatata
    28141 aaattcatct gataaaaatg caaatcgagt acctaatttt tcaagatatt tctgttgtat
    28201 ttgatgtatt tgctctatag ttttacttgc gctcttatca ttaaatatat ttaattctac
    28261 tagattttct ctatgctttg taatcccaac tggaactatc gcaacactgt ttacatacgg
    28321 ataaagttga gctagttctt ttattgttct atccaattcc tttccatcat ttatacctgg
    28381 acataaaaca atttgacagt tcatttctat atgagcatct gccaatctct tcattatacc
    28441 atataatttt cctgcaaatt tattgcttat cattttttgt cttaactctg gattagttgt
    28501 atgcacagat atatttatag gacttattct atattttata atattattta tatcttcttc
    28561 actcatattt gtaagtgtga caaaatttcc ttgtaaaaaa gataatctcg aatcatcatc
    28621 tttaaaataa agagtttctc tcataccttc tggcaattgg tctataaaac aaaacataca
    28681 cttatttcta cagcttttag cttggtcaat tatcggattt gtaaattcaa cacccaaatc
    28741 ttcatcatag tctttttcta tctcgtatat gtagacttcg ccatcttttt tttgtatttc
    28801 tacatctaaa tattcatcac ttagtaagaa tctatactct attatatcat gtattggctg
    28861 ttcattcaca gatataagta aatctcctac ctctataccc atttcttctg caatactatc
    28921 tttataaacc ttacttataa tattatttac tttttttaca tttacttcca atattttcac
    28981 cactcttttg tattcatcaa tgcttattat aatgtatttt aatatgcaat ttatttattt
    29041 attatatttt atgatagcat ttttaagcat ttcatgtcat aataacacat tttaattcta
    29101 atagtcaata taagtaaata gctataaaat agattagaac aaataatatt caaatttaag
    29161 tttcttaaaa atttaaatat tatttgttct taaaaaaatt ttataaattt aatgttttac
    29221 tatgattaac gtcccatttg tcatatcatg aaccattcca cctacacact ttgacatcaa
    29281 caatgcctta ttttgtaagt ctttctcatc atttgcataa aatattggac aacctccacc
    29341 atttttcata ttcttatcaa gtgtaactat agctagggta tattcattta aacctatatc
    29401 cattcccatc tcatgcactt cctttctttc ctattttata attttttagt gataagtttt
    29461 ttcctttaga gcttgaaagt attgggcatg actttacagc ttccattact ttttccacat
    29521 tcttatctac tggtaaataa gcaatcatta tactttcatc ctctttattt cttctaggta
    29581 atggtacgaa tgaaggttct ccataatctc catataaacc taaccttgaa tatatattgt
    29641 atactattgc ttgtctttga ccggagtcaa ataatattcc tgcattagca taatccttat
    29701 ttttaggaat tatttgtatt cctacacctc tattaatata tacttctcta tcgcttttta
    29761 gacctatatt tgttatacct tttaaatctc ccacctttag tatagattcg tccacaaaac
    29821 taatttcagc aggcattaca tcagctatgt ctccaatact ttttctagta agaagttttt
    29881 ttaatataaa ggctaacact aatccacaaa ttgaacttat taccaaactc atcccttcac
    29941 ttattttcgt ttctgcaagt attacataat acaatcctat tgttaaaaaa gatgtaacta
    30001 tacacatata atttcttact tcataggttc tagctatctc ttctatgaat gcagctcctc
    30061 tctttacaag ttgaacttcc tctaaatttt gcaatgtatt tcttctattt gctctcactt
    30121 gtctaaattg ttctgctgct agagataaaa aagttataga tgtataagaa cgatttataa
    30181 gagctggaac caataatgct cctaatgaag aagcgacgaa tgctaatata agttgagata
    30241 gtaaaacatt tggttgcgtt ggatactgtt tttggtctaa attcaacatt ataattctag
    30301 atgttatacc aataataaca gcaacaaaaa atactctatc cattaaatca ctcctaaaat
    30361 atttaatttg aaattttaca tgtaaatcta caaagtcttt ctagaaaaat cactctgctt
    30421 tccttttgat gtttctatga taggtgtact tttcactaca tctatcatga catctatatc
    30481 tttgagtatt ggcatatatg gtataaccac agtacttttt tctaaattgg tcttagatat
    30541 agcaacaata tcaacctcat ctacatctct atctatcccc atatgaataa atagattatg
    30601 tattatagct tgtctttgcc ctatatcatt tactattcca aatgcaccta aatctcttgg
    30661 tataacttct atggccagac attcatttct atacttttct cttgtacttt ctagacctat
    30721 gttagttatt attacaccat ttaccattaa aataggtcct tcaaaactta tttttgcagg
    30781 aactatatct gctatatctg ctatactgtt ccttcttaaa aatcttctaa aaatcaaacc
    30841 aacaatagct cctgacacaa tagctaggat tgtgcaataa aaaaagctaa ggccatactt
    30901 tcttgcaaaa acgatataaa ctatcgaaga taccagtgca gaaaaaagac ttatataact
    30961 tctagattca tacgtagatg ctatttcttc aacgtaagcg tctccttttg gtactaattc
    31021 ttcattatcg atattcttta gtgttattct ttcttgttct gcaagacctt gaaactgttg
    31081 tattgctact gcaaaaaatg tcaaggctgc aaactcctta tctataagtg caggtaatgc
    31141 tatagcacct aacgaagctg aaagcccaga tataattatc tgttctaaat aatcttgtgg
    31201 tcttgatggg tattgtttgt ctgtaactct taaaactaat cctctacata atattcctac
    31261 aactaatgca actataaaag agtgtctaaa taaatttgca ttcaataaac tttcttccat
    31321 tttctcagct ccttctctta tatagtttgt gcaaaaaaca aaaaaaattc ccttctattt
    31381 aaggaaattt ttttgtaata ttgtaactat aaattcgcta ccaatgccaa gttcactttt
    31441 tacttttata catccattta gtgatttgac tatatgcttt gttatagcca atcctaaacc
    31501 cgttcctcct acatccctac ttcttgcttt atctacccta taaaatcgtt caaatatgcg
    31561 ttctacatct tcttttggta tccctacacc attgtctata actttaatta cgattttttc
    31621 ttcatctttt aacactacaa cttgtacaat cccattatct tgggtatact ttatagcatt
    31681 atcaactagg tttaagaaca cttgctttat atagtcttta ttggatagta tacttattga
    31741 tttatcatca catttatata aaacttctat atttttttca ctagctgaat gttgagttat
    31801 ttcatatacc tctttaaata catcatatac tacaacattt tccataagaa gagtatcttt
    31861 attttctaca aatgataaca gcaatatatc atcaatcaac ctttttaatc tatctgattc
    31921 gctttctatt atttctaaga atctatttct agtattcaca tctatatttt catttgactt
    31981 tagtgtttca acaaacccac ttatagatgt aagtggtgtt ttaagttcat gacttacatt
    32041 tgcaacaaaa tcacttctca tattttcaag tttaactttt ttagtaatat cttctatatt
    32101 tactatcgaa cctattatta cgttgttagt attctgaaga taaactgggt ctacttttat
    32161 cttatatacg atatcatctt tagtagtaat attagcagtt tcattatctt tagaaccaat
    32221 aaattgaagt atttgtttta atatttcttc ttcttttatt acaacattaa tattatgacc
    32281 ttcaactaat tgatttggtt cactctttat catttctttt gcttcatcat ttatcagaag
    32341 tatatttcca tttacatcta tagccaaaat tccatgagat atactcttta atatagacct
    32401 taactgtaaa tgtttatact gtacttcttc taaggtttta tccatattct ttatcatatg
    32461 attaaaattt ctagctaatt cgccaagttc tcctttggct gatatgttaa gcctagcatg
    32521 atattcatta ttacttactt tttttgagac gtatataaac tcttttaaat atttccttaa
    32581 tgttattgtg tatcttacag ctaatataac aatggctatg gacaacataa caataaaaaa
    32641 atatatacta tttaaatctt ccacaagata acactcctaa aatataacct ttttaatcta
    32701 ttttgtatcc tacacctctt atagtctcta tatatttttc cgatttatct tcatcttcta
    32761 ttttttttct taaatatctt atatgaacat ctactgttct agtttctcca tagtattcat
    32821 acccccatat tttatccaaa agataatttc ttgaaagtac ttttccttta ttttgaatta
    32881 ataattttaa caattcaaat tccttaagag ttaactctat cttttcagag cctttggtaa
    32941 cttcatgttt tgataaatct agttttaaat tacctgtagt aagtatattg ttttcttcac
    33001 ctaagctagt caaattatat ctccttaaaa cagaactaat cctagctaac aactctttta
    33061 tactaaatgg cttagttata tagtcatcag cacctatttc aagcccttca accttgtcat
    33121 tttccatatt tttagcagta agcattataa caggaatatt ttttaagtcc ttatcacttc
    33181 ttatttttct caaaacttct attccactta tatttggtaa catccaatct aaaagtatta
    33241 aatctggttt aatttcctta gcctttataa atccgtcaaa tccatcatat gaataactaa
    33301 cttcgtaatt tgacacctcc aaattgaatt ttaatagctc aactatatgc atttcatcgt
    33361 ctataacaag cactttagta ttcattaaaa agctcctctc tattttatct gtagtaccgt
    33421 tcctattcgt tctgatgcct tattatgttt tctataagag tggaatttat ctgctctaca
    33481 actagtgcat aaatttaaat ttattatatt ttctttttta actccactac atctaagcat
    33541 atactcatta attttccata aatctaaata gtaacttccc tcttttatta tatagaactt
    33601 ttcatcatta tttgtaagaa ttgtgttaaa tttttctact aaatcttcag acacttcata
    33661 acaacatggt ccaattgatg gcccaattat acatactaaa tcttcaggaa ctgttttata
    33721 gcattcagac attaatttta ttgttttatg acctatatta ctaaaagtcc ctctccaacc
    33781 tgcatgagcc agtcctatgg ctttattttt agcatctata attgatatag gtacacagtc
    33841 tgctgtaaac agtaaaagtg gtactttttc caagtttgtt ataagtgcat ctccatctct
    33901 tcttttccct atagtatctt tattgactat attaactata tctgaatgta tttgagaatt
    33961 actagttaga ttttcaaatg tcaaatcaca ttctttacat acttttaaca tatcttctct
    34021 attttttgca tctatattct tacatgttat aactagattt acagaattta atttttcatc
    34081 aagttcttca caattaccac ttaaattaac atactcttta atatcttgta cattttttat
    34141 atttatataa tctttcatca tttgtctcct ttttctatca gtatactttt aagttcattt
    34201 acaaaagtat ttatatcttt aaattgtcta tacacagaag caaatcttac ataggcaacc
    34261 tcatctaact ctttcaattt atccattacc atctcaccga tttttttggt ttcaatttct
    34321 tctataaaac atttatttat ttcattttca atatgtacaa caatactttc aatctcgtca
    34381 atagaaacag gtcttttttc acatgctttt agcaatccat attttatctt ttctctatca
    34441 aaatattctc tactattatc ctttttaatg accattaaag gagttgtttc tattttctca
    34501 tacgtagtaa acctcttttt acatgattca cactcacgtc ttcttctgat agattttaaa
    34561 tctgtatgtc tagaatctat tacctttgat tctttataat tacaataagg acattgcatc
    34621 tatattcctc ctttgtttaa ctatatctca aactcatcta agcttaattc tgaacctata
    34681 tttactatta ttgtatcaca acctatttta agtatatcat tccaaaatat aacttcttca
    34741 tcccctcctc ttgaaaaaaa acttctactt ccatctccaa aaatggtaaa tgagactacc
    34801 ttaccttcgt gaatatccat gtctatgtct attataaacc ccatcctttt tccatttttt
    34861 acatttataa tttctttttc cattatatca gaaactctaa tcatattgta ctcacccttt
    34921 ctttaaaaat aaatatgtat cgaagtaaaa aaaaatgacc tatataggtc atttaaatta
    34981 aaataatgtt tacagtccat ggattactta tacatatttt ctcatatttt ttaaagcatt
    35041 tttttcaatt cttgatactt gtgcttgaga tataccgatt tcatcagcaa cttctatctg
    35101 agttcttcct ttgtaaaacc ttaaatctaa taccaacttt tctctactat ttaatttctt
    35161 aatagcttct ttaagtgata tttcttgcaa ccaattttca tcagtatctt ttttatcttg
    35221 aacctgatcc ataacgaata ttgcatctcc attgtcttga tatactgggt caaacagaga
    35281 tattgggtct tgtatggcat caagtgccat tacaactgat tctacttcta attctaattc
    35341 ctttgctatt tctgatactg taggttcttt agaatttgtt ctaattaatc tttctctaac
    35401 ttgtagtgcc ttatatgcta tatcttttaa tgacctactt actctaattg ggttattgtc
    35461 tcttagatac cttctaattt ctcctataat cataggaaca gcataagttg aaaaccttac
    35521 attttggctt aaatcaaagt tatctatagc ttttataaga ccaatacaac ctatttgaaa
    35581 taaatcgtct atattttctc ctctgttgtt aaactttttt attacactca agactaatct
    35641 taaattccct cttacaaact gttgtctggc ttcttcatca ccatttttta tttgtaataa
    35701 aagttccttc atttgcttat ttttaagaac tggaagttct gatgtattaa caccacatat
    35761 ttcaacctta ttaacttgca tatattttca gtcctctctc aaaagtattc atctattgaa
    35821 attatttaca tttccaattt ttttatactt tttaagaaga attaatttag cattatacaa
    35881 attttttcat ttctttttgc aaccttgaga ttattttctt ttcaagtcta gatatgtagg
    35941 actgtgatat tccaagcatt ccagcaactt ctttttgtgt tttttcaata cctttatcaa
    36001 taagaccaaa tcttaattcc ataatttgct tttctctgtc acttaatctg tcaagtgcca
    36061 taaccaacaa atctctatct atttcttctt caattatttt gtatatttca tcattttctg
    36121 tacccaatac atcagataat aaaagttcgt ttccatctaa atctatattt aaaggttcat
    36181 caaatgatac ttctgttttc tttttattat tttttcttaa gtacattaat atttcatttt
    36241 ctatacatct cgaagcataa gttgccaatt ttatattttt attcagttta aaagtattaa
    36301 ctgcttttat caaacctata gttcctattg aaatcaaatc ttctacatct atacctgtat
    36361 tttcaaattt tctcgatatg tagactacta atctaagatt tctttctatt aaaattgact
    36421 ttacactttc atctgtttct agcttttgta aaagctccat ttcttcttct ggtttcaatg
    36481 gaggaggaag tatatttgcc cctcccatat agtatattcc ctttggcttt attattttta
    36541 atttaatacc taacatagta ataaagctta ttattctttc tttcaatcgt aacattcctt
    36601 gtccccctta aattttcacc taaaatccag gcaatatatt tggattcatt attgctccat
    36661 attctctatc attaaaattt gaaataccaa gtattatatt tcctatcttt tgctcatcta
    36721 cttctatata gtcagctttt aagcctatta ttatgcttgt cttattactt ccagcatgtt
    36781 tataaggtat tattctaact ctacctgaag ttttatcatc taaacttcct ataatttctt
    36841 gtgctttctt catatctata tcttcataat taatatttaa tagtttttct ggaataaatc
    36901 cttctaacac actagactta actattatta catcactttg acttattggg tcttttaaca
    36961 aattaccact atctactaaa gccttacagc aaaaactttt ttctagtaag tttatattta
    37021 tagtctttgt aagctcctta atgtacttaa gcattttcaa atctttataa atgtatttta
    37081 ataattcaca actcacataa gtacatgcaa ttaagaaaga tatttttaag tgacttattc
    37141 ctgtaaaata aattataaaa taagtgcttc cagaaataaa aatgtttact agataaaaaa
    37201 ctaacgctac atgaacataa tcttttttgc tcttacttct aaatgaaatt aaagttatta
    37261 tactcatgat tattatttta aatggtacag taaatagtat attcattgat ggatacaaat
    37321 aagcaacaga ataggcacct cctaaactag cccccaataa cttatttttt ttaggattat
    37381 atcttttagt aagaatagaa gtacaactta taataatata atttattact aggttttcta
    37441 taacatagta ctcaatatac accatgtatc cccccttaaa acctttaaac atattataag
    37501 atagtatgta caaaatttta tgtcactttt gatggtcgaa tccaaaacaa attctattta
    37561 aaaagaacaa tgatatatgt cttatacttt atgtcgtcaa aagatatttt tttaatacca
    37621 aacaaataga cttttttgta gaatccaata tatctaggta gaagttattc actctacttt
    37681 tggtatattt acaaaaaaag actacataat ttaataatat atcaagtaaa tcttttactt
    37741 atataaaatc aaattatata gtctgtaaat tcaaatataa attattgtgc tagtaatcca
    37801 tttgcatatt ttgcagattc atttagtgct tgttctggag ttatgtctcc agctatcatc
    37861 atttgaagtt gtttccataa atattcatat atctctactc catgaggtcc aactgtcaat
    37921 ggtctataca tatgtttatc tcttgtcacc atttcttcaa atcttgggtc tccttggtat
    37981 ttttcacctt tagcaactgg agctctaggt atttctttgt ggaaagcttc cattgattct
    38041 ttgcttagca tatactcaag aagtttaaat ccaagttctt tatttttaca tgctgacatt
    38101 aatgttaaat catctactga agcaaaactt ccaactgtct tatcttctaa tgatgttaca
    38161 tatccccagt ttaaatccgg gaatgattta tcaaaagtac ttgtagcagc agaggatagc
    38221 attatagtaa atgcagcttt tcctggtcca aatacacttt caatcatttc attactatct
    38281 tttgataaag catcttctgg aatatacttt ttcatatcat ttataaagtt tgcagctttc
    38341 aagccttctg cattattaaa tttaactgat tttaaatcat cattatatat atctccacca
    38401 gcttgccata aatacggata ccagttccag tttaagcttc caaatacttt agaaccccaa
    38461 ccttgtgcaa gaccccactg gtcgattttt ccatctccat cagtatcttt tgttgctttt
    38521 tcacagattc ttttaaaatc atcccatgtt tttggaggtt tttctcctaa tttttctagt
    38581 atatctttat tgtagtacaa tacgcctgga ttagcagctt ctataggtaa accataaata
    38641 ccacccatca tttttgcatc atctaagtat agataattat ctgaagttcc tgatttttct
    38701 aaataaggtg ttaaatcttc taccgcaccc atttctataa attgaggaaa catctctgca
    38761 tacatgtatc ctatatctgg accttcacct gcacttattg ctgttgcata tttttctgcg
    38821 tagttatccc atggtactat ctgaaattca atttcacagt tattttcttt ttcaaatttt
    38881 tcaaatatcg gcttccatgc atctgcatca tggtctgtta atggtgcaga ccatacaact
    38941 aatttttctt taccctcttt gcctttagaa tcttcttttt ttgaacatcc tgcaagaatt
    39001 gatactgata gaatccctac cataactaaa gaaagaacct tttttagttt aatcatgctt
    39061 aatccccttt cagactttaa tttgtctttt tcctcaaatg tttacattta attcagattt
    39121 aatattaaca aataagatta ctcttgtcta atcataaaaa actatactaa ctcctataac
    39181 tatatttttt ttctatacct attagattta attcatacta aaaacttact ttttaaattt
    39241 tatcaatatt taaaaatttt tttaagtcta gccacaaact aactatctaa attgagcaaa
    39301 ataaaaagaa gtctattgac ttctttataa gcttacatat tatcttcttc ttcttaggaa
    39361 tgtaggtatc tccatgctct catcatcatc ttcttcaatt atacttgcct ttttaggagg
    39421 ctctattttt tcctcagcaa ttacttcttc ttcaatttct tttacagctt gttttactgt
    39481 tgtatttaat gaagaacgta tgcttgattt aggtttagta tctaaatcta aggcaccatt
    39541 ttgtaatcct tcaaatcctg tcgcgattac tgtaatcatt atttcatcac ctaaatcttc
    39601 ttttatagaa gctccaaata taacatttgc ttctgggtca caggattcca taactaaagt
    39661 tgatgcctca ttaacttcaa ataatcctaa gttaggacca cctgtaacat taagaagtac
    39721 tcctttagct ccttgtattg atgtttctag aagtggagat tgaatagctt gtcttgcagc
    39781 atcaattgct ctagtttcac cactagcact acctataccc atatgagcaa gtcccttgtc
    39841 tttcattata gtagtaacat ctgcaaagtc caagtttatt aacccttcaa ctgctataag
    39901 gtctgaaatt gattgtatac cttgttttaa tacatcatct gcaactgcaa aagcatctaa
    39961 cattgatgta tttttttgta ctatctgtaa aagtctatca tttggtatcg ttataagagt
    40021 atctactttt gatttaagtt ctgctattcc accttcagcg ttcttcattc ttatcttacc
    40081 ttcaaatgca aaaggtttgg taactacacc tactgtaagt atccccattt cttttgccaa
    40141 gcctgcaacc actggagcag ctcctgtacc agtaccaccg cccattccgg ctgttacaaa
    40201 gaccatatca gctccttgaa gtaactttac tatttcatct ttactctcct cagcagctct
    40261 tttccctact tctggatttg ctcctgctcc aagtcctcta gttaattttt ctcctatttg
    40321 aactttatat tcagcttttg atgtatataa agcttgttta tctgtattta ctgatataaa
    40381 ttcaacgcct tttagttgag cttctaccat tctattgaca gcattgtttc caccgccacc
    40441 tacacctatt actttaattt gtgcacattc ttctaattct acgtcaaagt ttagcatcat
    40501 aaaccccctt ctctcatgat tcaaagttat cgtatgtatt aattatatca ttttcttctt
    40561 ttattttgtt aacctttaag taacatattc cacaaaaacg taaattttcc tttttaaatt
    40621 tcttacttat attgtcaaat attactcttt aagtataaaa taatttacgt atatattaaa
    40681 tgctctttaa tttttacgtt ttttgttcat aatattacta ataatatatc gtctaattat
    40741 agataagtta ttaaacaatc tcataccaaa aactaataca gcaacatagt aaagagaaac
    40801 tcctaacttt tctcctatat aagtcaataa tatagccaat atagcattac ctatgaaacc
    40861 agtaataaaa attacattat catagttatc ttcaaaattg gattttatcg ctccaaaaat
    40921 agaatccatt attgcaagaa tagctactga catatatatg gaataatcca tagaatatgt
    40981 aaatggaata tagtaaccta ctactacacc aaatgctaat cctcctaaaa cccataacat
    41041 aaatcttcac ctctttattt tactttgaaa cacaactaca ttacaatcct ttttattaat
    41101 tttttttact ttttctttat ttttttgtat ttttcgtttt attttgcaaa taaatccact
    41161 ttatttccat tattttcctg aacttttata ttaattccat atatatcctt aagagattta
    41221 gcatatgatt ctggagagtt tatagctgca ctcaataaat ctatatctcc tatagctttt
    41281 attataaatg gttgtccata tgtagtatca tttactgtaa tagtagctcc tgaacatttt
    41341 atcttacttg ttgctagtac tctttctcca tttatagaaa ttgctcttgc tccagccttt
    41401 tttaaatcat ttaaaaccct caaaatatct atatcatgta taatcaaatc gtttggattc
    41461 tgtccgtctt ttaactctct ctcactatcc ctcatagtaa tagttatgcc tggccctgta
    41521 acagttgaat aaccacttaa ttctttaact tcttcaagct ctttttgcat aagtgtttgg
    41581 atagattcat ttccattctt ttttgcttct ttatattcat tcaaagtttt tttgttgttt
    41641 ttaagagttt cttttaaatt ttttatttct ttttttgtat tttctacctc actttcaata
    41701 ctttcagttt gacctaatgt tatgtatact ctatttgggt ctagtgattt tatataagtt
    41761 gatatgaaaa cgcctaatat gaatgctcct aatataacta ttttcttttt catactatta
    41821 tttcccctct gctttgttta tatcactatg accatttatt tctatattgt cttctttggt
    41881 cacttttact ttggcattat aatatgtttc tatttcccag acaataccat actttatatt
    41941 aagtgccgaa gccaatgtat cagaatcacc aattgctttt attataattg gttcttttat
    42001 gacagtgtca ttcatataaa gcttgtcttc ttttaaatac atatatgtat tagaaactat
    42061 tctctggtcg tttatagata tggcacttgc ttgtgctgaa tttaatttat ttatcattga
    42121 caataataaa tcataattat atagtatact atcattttta tcagagtttc ctaagtcttt
    42181 tgtagaattt atctcaattt ttatgcctgt tccttcaaca tctgtatatc cagctaatgt
    42241 ttcatatttt tctatttctg acttaagtat gtcttgttct ccttcattgt ttttgcctct
    42301 ataagtctct atatctttct tcacactatt aacttctgat tgtagattct ctttttcttt
    42361 ttttaaagat ttaagttctt ctgtaagttg ctctcctctt ttaaaagttg tcatccctct
    42421 attttctata ttaataattt taagttgtaa actaacaaga acacccaata caatacttcc
    42481 tatgattata cttttattta taaatctttt tttcaatata tcacttcctt acaattgtct
    42541 atccttctgg actatacact gccaactttc catagttaag atttatagtc cctccattag
    42601 tatttttatt ttgcaggtca atcagaattt tacttaccct agatatatta taatctaagt
    42661 tatcatcatt agaaagtaag atttttatat tacttcttgt aagcatttct atattgcttt
    42721 ctttttttaa gtttacataa tttatttttc tataaattcc ttcttctttt aaataattta
    42781 aagttttaaa tacattctcc ttagttttat aatttttaaa ttttatagat ttattatcat
    42841 caatgctata atctacatct actatcaagt cttttttact ttcattagaa cctcttagtt
    42901 cttccaataa atttcctttt ttatctatat aacaataatt atcttcatct tttaatactg
    42961 caaatatttc tttttctttt aaacttataa ttatcttatt gggaagtttt ctttttattt
    43021 ccacattttc tatatatgga ttttttataa gtttattttt catatcttta aaattgtatg
    43081 caaaaatatt ttcatttaga tttacattta gttctttcat tatatttgat ttggtcaccc
    43141 tcttatttcc aattacatct attttcttta catcaaataa atcacttttt acaattatac
    43201 atatacaaaa agataacata agtataaaaa ctaaaactat catgacatta ttcgtattta
    43261 actttcttct ttttttcaaa tataatcaac ccctttggtt aataaataca cctttagagc
    43321 ttgtttttcc tacaaaaaaa ttataatatt taaagtcaaa tttttgcatt ttattacata
    43381 acatccaaat aaggtattaa attttataaa taaatcaata tttcatcaaa ataaacatag
    43441 catactacat actatttata tatttaaatc tgtatacata caaataaacc cctaatttat
    43501 aataatttag aaaatctaat atattataaa ttaggggtaa atatttactt atcttttctt
    43561 tattcctatt accttagctt gaccttctat acttggagtc gtttcttttt ttatttcttt
    43621 tacttcctta ataacttttt ctttttttgt ttttttagaa gttgactttt gtgttgaatt
    43681 gtatactttc attatctcat catatataag gtctatggct tcaggcttac ctatagtttt
    43741 acttgcattt gccatatcaa ccaaaagttc tctatctcct aaaagtttaa atactgctgt
    43801 atttaggctc tctggagtta gatttttttc taaaatagca attcctgccc cttgtttttc
    43861 tatactcttg gcattatact cttgatgatt ttcagcagta taagctttag gtattattat
    43921 agatggcttt ccaagagccg ttatctcagc taatgaaatt gctcctgcgc tacctataac
    43981 caaatcactt gctgcaagtg catttgccat atcttctaag taaggcacaa ctttttggta
    44041 tggtttaaga tttatatcgc taatacttcc cataaattca tcataatagc ttttgcctgt
    44101 agcaaatata aaagctatat cttcatttac catattcttt attacaagtc tcattgcatc
    44161 atttattttt cttgaacctc cactaccacc atagcaaagt accattcttt tttcgtcact
    44221 tattgataga ttctttcttg caatattttt tcttgataat aaaatttctt ttctaactgg
    44281 atttcctgta aaaactaatt tatcttctgc tgcctctgga aatcttttat gcgaatcttc
    44341 aaaacttgta agtacctttg taactgtttt agataaaatc ttattagtta caccaggaaa
    44401 agaattttgc tcatgtatta tggctggtat tttccccata gatgcattaa ataaaacagg
    44461 tccactaaca tatccaccag ttcctataac tatatctggt ttaaatttct taacaatctt
    44521 tcttgattgc tccaaacctt taaaaagctt aaaaactctt tttacattat cgaaatctat
    44581 ttttcgctta aaaccttgaa ctgttacagt ctttaattca aatccatact taggaactat
    44641 ttctgactct attccctttt ccgtcccaac aaaaattatc tcagcgtctg gatgctcatc
    44701 tcttattttg tttgcaatag ctatagctgg atacacgtgc cctccagttc caccgcctga
    44761 taataaaact ttcatcaaat catcatctcc tgttaattaa ttttgacatg ctttgagatg
    44821 tttaacacta ttcctacagc tcccataaag atggtgagtg aagttcctcc ataacttata
    44881 aatggtagtg ctacacctgt agctggcatt gatgatgtcg caactgctat atttaaagct
    44941 gcttgtattc caatttgtgc acctatacct ataacaacca tacaagcaaa tacattagaa
    45001 gtcttcaaag ctattcttac acatctgtag actaaaacta caaatagcat aataactatt
    45061 atacaaccaa tcaatcctaa ctcttctcct attatagcaa atataaagtc attttgtggc
    45121 tctggtatat aaaaatactt ttgtttactc tttcctaagc ctagaccaaa caatccacct
    45181 gaacctagtg catatagacc ttgtattacc tgatacccct taccaagagg gtcttgaaaa
    45241 gggtctaaaa atgatgtaac acgactaagt ctatagggtt cagcaattac taatgcagca
    45301 aataaagcag ccccactccc tatcatagca aatacaattt tcatatccat tccagcaaca
    45361 aatatcatta caaaagtaac tagaattaca gtacctgctg ttgacaaatt tggctgaagt
    45421 attatcaacg caaaaaatat ccctggaact acgagaagtg gtacaacacc ttttgtcaaa
    45481 gattttattt tatcataatt tttttcaatt aattttgcag ttaatattat tgtagcaaac
    45541 tttgcgattt ctgcaggttg aaatgttaaa gctcctattc ccaaccatct ttttgctccg
    45601 tttgcttcta tccctaaagg ggtcaaaact aacaataata atactactgc tatagctcca
    45661 attgctgtag catttttctt ccaaaaacta taatctattc ttgatgtaat aatcataact
    45721 ataaatccaa gtacagcata tattacattc ttttttagaa aatagtacgc atcattgtgt
    45781 ttaaatgaag attgtataaa acttgcacta aacaccataa ctattcctac aaatactaaa
    45841 agcatagtag tgtaaaacac tactccatca aattcagtct tcacccttat gtcaatttgt
    45901 tttttcaaat tttctttagg catccaacta atctacctcc attcttccat ttaagtaagt
    45961 ttagaggtaa gagattattt taaattgttt acattatctt tgaagtcttt tcctcttact
    46021 tcaaaacttt catacatatc ccaacttgca caagctggtg aaagaagcac tatatctcca
    46081 ctttttgaaa tttcataaga agtcttaact gcttcttcca tatcttttac tatatgtatg
    46141 tcattaaatc ctttattttt agcacaattt tctatatttg atgcagtttc tcctaataag
    46201 accaatgatt taacatatga ctttgcagtt tcaagaagtt catcaaaatt acttccctta
    46261 tccatccccc ctgctataag tattataggt ctatcataag actgtatagc ttttatagtg
    46321 gaatctggat tagttccctt tgaatcattt acatatatta cattgtctaa atttcttaca
    46381 aactcttgtc tgtgttccac tcctttaaat gtcataagta catcctttat aacttctaaa
    46441 tcaattttgc agacatatgc tattgctata gctgccatac aattttctaa gttatgacct
    46501 ccaggtaaac ttacatcatc tttatttaga aatttgattt tatcatctat atctattatt
    46561 atgtcattat tttctaaata tactcctcca tttacttttt tagttcttga aaagtaaatt
    46621 actttggcat tgcatttatc agctaaagat tttactatct catcatcata gtttaataca
    46681 cagaaatcct caacagtttg gttcataaat atatttgcct ttgcttctat gtatttttcc
    46741 atagtatggt gtctatttaa atggtcctct gtaatattta atattgcgct tactttaggt
    46801 ctaaattcat ctatactctc aagctgaaaa ctgcttagtt cagttactaa cacagactct
    46861 tcacttgaag tttctatagt atctattaca ggatttccta tattaccaac tacatatgta
    46921 tctcgttttg ctcttgaaaa aatttctcct accaaactag ttgtagttgt ctttccattt
    46981 gtacctgtaa tccctataaa aattggatta tccgatagtt taaaagctaa ttctacctca
    47041 ccaattatat acttgcctga attttttaat ttcaatatga atggtaggtc tagtggtaca
    47101 cctggagaca ctacaaccat atctatatca tctacatctt ctgggtgatg tcctaatatg
    47161 tattttatgt catttataga tttcaattca tcaagtatat ttctcaattt attttcatct
    47221 tttatatcat ttacgattat acttgcaccc aatttatcta aatgtttaat tgtagatata
    47281 ccagtttttg caagtcctac taataaaact ttttttcctt ttaagtccat ttttcattcc
    47341 ccctagccct aaaatactgc tattatactt atccaagcta aaacaactgt tataatccaa
    47401 aaaataaata caactcttgt ttctggccaa ccacactgtt caaagtggtg gtgtattgga
    47461 gccatcttaa agaatctctt tcttgttgcc ttaaaatatc caacttgaat caaaactgat
    47521 agagcctccg caaaataaat tcctccaatt ataggtatta taagcactga gttagttaga
    47581 actgaaaatg ctacaactgc acctcctaat gccattgaac ctgtgtcgcc cataaacact
    47641 ctagccggat atgaattaaa tcctaagaaa cctaaacatg ctccaacagt tgctgctgct
    47701 aaaactgcga cttctgtatt tccagcaata gaacttgcaa atagcataaa gaatacagaa
    47761 actattaatg ttactcctga tgctaatcca tctaatccat cagtcaagtt tacagcattt
    47821 actattgcta cgattataaa catcattatt ggtatatata atattccaac atttatcaca
    47881 aaatctgtaa atggtatcat aagttgtgaa gcacttgatg atgatgtata ctgataaaaa
    47941 gcaacataaa aagataatgc aacttgaagg attatttttt gatatgcttt tagtccaagt
    48001 gatcttttta gctttatttt tataaaatca tctaaaaagc caataaaacc aaaacctgca
    48061 atacatataa gacctactgc catatcatga ctaactttta ctcttgtaag tccagttatt
    48121 agaattgcaa caatcattat aattccaccc atagtaggtg ttccgttttt agctagatgt
    48181 gtttggggtc catcatctct aactgtctga ccaaatttaa attttctaag cattggtata
    48241 aatattggtc ctattataat tactattaaa aacgcaatca atgcggtgta cgtaagctct
    48301 gttattccta acatcataaa actcctctcc cttgtcaatt aactcattag tttaagctta
    48361 tttatttaga tattcgacta ttttttctaa tctcatgcct cttgagcctt ttactaaaat
    48421 agtgtctcct gtttttacta attcatttaa cttgttaaac acatcttctc tattctcaaa
    48481 gtgatatata ttggctgaat tgaatcctaa ttgttttgct tcttctccaa taaacactga
    48541 attttcacct atagttatta taatatcagt attattcata gatgattttc caactaatct
    48601 atgaccatac tctgaaattt cacccatttc tagtatatct ccaagtatcg ctacccttct
    48661 acctttatat cttccaagta tctttaaagc agcatccata gaatctggac tagcattata
    48721 tgaatcatta attattgtta atttttcatt ttttattatg tctagtctca tttttgtccc
    48781 tttaaaattt tttaaacctt tttttatata atctaaagaa atatttagac acaaaccaac
    48841 taatatagca gacattgcat tatatatatt atgttctcct attgttggta taaatatttc
    48901 ctcttttttc ccatctatta cacatataaa tgttaaacta tcctcttcca tagtataact
    48961 ttcacaataa acatcattgt ttttattaaa gccaaatgtt tttaacttat atacatgttc
    49021 tttttctttt aatgtagata aaaacttatc atctccattt acaattaaag tatttgtttc
    49081 atcaaagttt gaagtaatct ccatctttgc tttcaaaata ccctcttgtg aacctagttt
    49141 ttctacatgt gataatccta tattcgatat gactgcaatt ttaggattta ctatatccac
    49201 taaatatttt atttcattga atccagacat acccatctct attacagcac actcatgttc
    49261 tttatttaga ttaaataaag tcaatggaac tccaaaatga ttatttaagt ttttttcatt
    49321 ttttagtata tttaactttg cagatatggc agagtaaatc atatctctag tagtagtttt
    49381 tcccacacta cctgtaacac ctataaatgg aatatcaaat ttttctttat agtattttga
    49441 aatatctcct agagccaaag atgtatcttt tacctctatt aaatttatat ccgaactttc
    49501 taattttatt ccattacttt cattttttat aaaagtttta catccactat catatgcaga
    49561 cttcataaat gtatgcccat ctaaattttc tccaactatg gctacaaatg cattttgggc
    49621 atttgcctcc ctgctatcta taactatatc acttacacag tcattataat ctccatatat
    49681 taacttccca ttagttgcaa gtactaactc ttttattgtt aagcactcca ttattaaact
    49741 cctcctttaa aaccttagct atattctctt agcaacaaaa ggcaatgtat accgttatat
    49801 atacattgcc tacgataact cttaacttta taattatata ctatatttct gtttttttga
    49861 agatgtttat gggtatatgt taattatctg ttacttttta gttttcaaaa tatagggtta
    49921 tacttgagtc ttcattaact tttactccag gttttgggaa tatatccttt acctttgtat
    49981 cttctggaag ttctatatct gcatctaagt ctggttttaa cttcgcatcc tccaatgcct
    50041 ttacagcatc tcctattttt agatttctta catctggcac tttaacttgc ttcttttcat
    50101 actcagcttt ttcttcttct ttatatactg gtttaactcc caaatatttt aaagagtcat
    50161 tcattatttc tttcactatt ggtcctgctg tagtactacc aaatgcactt actcctgttg
    50221 gttcgtcaac aatagcaaga actactattt gcgggtcatc acaaggtgct attccaacaa
    50281 atgaacatat atactttcct ggagcatact taccatctat aactttttga gctgtacctg
    50341 ttttaccacc aagtctataa cctggtatat acgctatctt tcctccacct tcagttacaa
    50401 ctgattctgc aatttctaac atttttttag atgtttcttt agatataaca cttcttactt
    50461 tttttggttt tactgtttca gttatatttc ctttattgtc agtataagat tttaccactc
    50521 ttggttgcat taaatctcca ccattagcta ttgatgatat agctgttata agttgtattg
    50581 gagttactga tatagattga ccaaatgata ttgtagccaa ttcaactggt cctacatttt
    50641 tttcgttata cagtattccc ttagcttctc ctggtaaatc tatacctgtc ttatccataa
    50701 gaccaaatga ttctatataa tcatacattt ttccaacacc taatctgctt cctaactcaa
    50761 caaatactgg gttgcaagag ttttgtactg cttgtttaaa ttcttgtgtt ccatgaggtc
    50821 tgtaatgtct ccaacattta atctttcttc ctccaacagt tacactacct gtacatgtga
    50881 atttttctcc atctttaatt actccttctt caagagcact ggaagatgta ataagcttaa
    50941 atgtagaacc tggttcatat gtatcactta ctgctggatt tctccacatt tgataatagc
    51001 cttttatttt atctttatca ttatattttt caagttcttc ttgataatat ggatatattg
    51061 gcgtccttga gtcattaggg tcataatctg gttttgatgc taaagcaagt atatcacctg
    51121 ttttagggtt catagctatt gcagtaacct tttttgcatt atttaactca taagctttct
    51181 gtactgcttt ttcagtatag tgttgaatta cttcatctat agacaacaca agaccattac
    51241 cttgtacagg ctcataatat ttttccattc cttgtggtat ttctcttcct gaagcatcag
    51301 tacttactat aagttttcca ggctttcctt ttaacttttt atcatattgc atttctacac
    51361 cagatatacc tgttgcatct gaagaagtat gtcctaacac atatggtgca aaattcccat
    51421 atgggtaata tctttgatta tcttctgcca cccatattcc acttaattta gcatctctta
    51481 tttgacttgc cttatcatca tctatccatc tttttacttt tacaagagcc atattttttt
    51541 tgcttattaa agcgtaaata tctttataat cttcatccaa aatctcagct accttttcgg
    51601 ctgcttcttt tttatcttct acttccacag gtttacacca aacagtatac ttagttacac
    51661 ttactgctaa ctctttcata tttctatcat atatagttcc tcttttgggt tctattggta
    51721 tatctcttgt ttgttgttct agtgcttttg tactcaacca atttcccttc ataagttgta
    51781 gatacccagt cctaataaca aggcaaaaaa acaatgcaca tgctagaata agaacgagta
    51841 ccagcctttt cttacttatc ctctttactt ttctcaaatc tattccccct agttatccac
    51901 atctatatac tttatttgtc catcttttgg atagtccata ttgagcaatt ccttagcctt
    51961 tagctcaatt tcttttattg atgttttttc agtttctttt gcctttagtt cctctaatac
    52021 tatctctttt tttcttaaat ctttttgtaa ataatagata tcgtacttca tttccgagat
    52081 ttttgaataa ccacatagat tcaatattat tactgaaaaa gttgcaatca gtaaaatgca
    52141 tttagctcct atttttttct tatttctctt tttataatta ttcttttttt tatttttata
    52201 ttcatttaga ttatgtattt tataattttt tttcaaattc tcaccctgat agtatatagt
    52261 ttaaattttc ctctatacta tatttgttat taatcatatt tcttcctcta aaatttcatc
    52321 gctaaattaa tacactatta cacaatctaa ttgtattttt ttctatacac aacaaaaacc
    52381 tataaattca cttatacttt tatatgaatc tataggttag atattaattt ctatttattt
    52441 ttatcacttc tagtattttt agataatttc ttccataaat ataatatgta actaactatc
    52501 tactctctga ttcctaagtt ctccattata ttacttaata ccatctatat ctatattttt
    52561 atatctaaat tttcattata tatctcaata acaaagaaca tttcttatag tgttaattgt
    52621 tttttaattg ctttaccagt gcaacaaaat atataatact ttttaattaa ttataataat
    52681 cttatatata agattattct ttagccttat aactacttat attttttctg ctactcttag
    52741 cttagcactt cttgaacgtg gatttacttc tatttcttcc tcactaggta atataggttt
    52801 tctagttatt attttcacct cagattcttt atcacattgg catataggta aatgctgtgg
    52861 acatatacaa tctgatgcta aatgcttaaa tgcatttttc actattctat cttctaatga
    52921 atggaatgtt attatacata ttcttccacc ttcattcatt atagaagaag catcatttat
    52981 catctttgta attactccaa gttcattatt tacttctatt cgtattgcct gaaaagttct
    53041 tttagctggg tgaggtccat ctattctggc tttcttaggg atagcttttt taattacatc
    53101 tactaattct cctgtagttt ctattggttt gtttgctctt tcttcaacta taaatttagc
    53161 tatacgctta gcccagttat cttctccata atcttttatt atcttagtta attcatcttc
    53221 tgtataagta tttacaacat catatgcaga aaactcacac ctaacatcca ttctcatatc
    53281 aagtggtgca tcttgcatat aagaaaatcc tctatcagct tcatcaagtt gatgagatga
    53341 aactcctaaa tcagctaata caccatcaat tttgtaaact cctatttttt ctaattcctc
    53401 ttttatattt tcaaaattgc tgtgaacaaa cttcactcta ctttcatatt cactcaatct
    53461 ctcttttgct gtgcttatag catttttatc ttggtcaaac cctataaata atcccttatc
    53521 tgacagtttt tttactattt ctttagaatg tcctgctcct cccatagtac agtcaacata
    53581 cacgccatct ggctttatat ttaaattctc tatacactca tttaaaagaa ctgatacatg
    53641 atgaaattcc atagttacct cccttaaatt cactttttaa actattcttc actaatatta
    53701 tttttatttt tctttgataa ataaacatgt gcaattatgc caccaataac tccaattaga
    53761 cttataactt gtgccattct aagtggtcct aacattaaac tatctgttct taatccctct
    53821 ataaaaaatc ttcctattga atataaagtt atataagtaa ctataacttg accttcatat
    53881 tttttatttt ttctaaatag taataaaact ataaatattc caaaatccca tatagactca
    53941 tataaaaaag ttggatgtac ctttactcca tcaaccatta ttccccaagg taaattggta
    54001 gcacctccat gtgcttctcc attaataaaa tttccccatc ttccaatagc ttgtgctaaa
    54061 ggcattccaa gtataactgt atcagccatt tttaagaagt taatattttt aatcttagtg
    54121 tatatataac cagctaatat tccaccaatt aaccctccat gtatagcaag tccaccacct
    54181 ctaaaattaa aaatttgaga catattttga gcatagtaac tccaattaaa tacaacataa
    54241 tagattcttg cacagattag tccaactgga attgctatta tagctatatt taatacatca
    54301 tcttctttaa ttcccactct ttttgcttct tttattgcta ttaaagttcc taaaatcatt
    54361 ccacatgcca taagtattcc ataccacatg atgtctatgc caaacagtgt aaatgctact
    54421 ctatccataa cgtcacctct ttgtattaat tgaaaatatt tccaaagcaa tatgctataa
    54481 ttacaattaa ttgtaattat actaaattat gataacacat taatgttaat tctacaaaaa
    54541 atattaaaat ttattctaca actccctaca ctatattata tctaattact ataaaatttt
    54601 ttaaactaaa tctctcatga ctaaagttac aagtgtacat agatattttt taatcaaaaa
    54661 gattgatgca tatatacatc aacctcttct aattcttatc tatagctctt aaatttttac
    54721 ctcaatttat ataaatatat tttataaatt tgttttacca aaataaacat ttaaaaacat
    54781 aaactaatac ttaattagtt attcttatta tgattaagtt tctatttagg ctctacaata
    54841 gaaataacac aatactcttc tttaaaatgc tcttttaatc tttcttcaac ttcctcaaaa
    54901 gtaatatctt taataatatc taaataatcc aatagattta tatccttaaa aacataagat
    54961 ataaaactat tacctataaa gtttatagaa tcaaagcact taataaatga ccctattttt
    55021 ttctttttag ttctctcaaa ctcttctttt gataatcctt ctttttttga tttttctata
    55081 taatcaagaa ttatttcttt tactttttta ggctctttag aatctccagc tattatgcta
    55141 aaagcataat ccacttgaga cgaaaatcca gctccaaaat tttcatttat taaaccttgc
    55201 atatacaaat cttcataaag cttacttcct tttttaaata acatccctac aagtatatca
    55261 gttacaatct ctttacgtaa aagttctttc ccttttaaac caacattgct atctttaaaa
    55321 cctatgttga acataggcat agatataggg aatttttcta taacctcttt ttcttttaca
    55381 ctttctggtt cttctggata aaatctttct atactcttag agagtctgtc tactttgtaa
    55441 ttgtttgact ttttagttac atctataact ttttctacat ctaagtcacc aacaacaaat
    55501 aaagccatat ttcctggatt atagaaagta ttataacatt tatatagctc ttccttagtt
    55561 atcttatata tactatcaac agtacctgct atatcaattc tagcaggata atttacatac
    55621 attgccttca agcaattaaa gtatacattc caatcagggt catcattgta catttttatc
    55681 tcttgtgcta ttataccttt ttccttttca acattttcat ctgtaaaata aggtgtttga
    55741 acatagtcaa ttagatgttc taaactctca taaaaatttt cagtagcaga aaataagtag
    55801 gctgtcattg taaaattagt aaatgcattt gcatttactc ctaattttga aaatttatca
    55861 aatgcatctc ctccatcagg ttgctcaaac atcttatgct ctagaaaatg tgcaatacct
    55921 tcattcactc taattttctt atcttctcca attggtacaa attctaagtc atttgaacca
    55981 taattagtag ccaaaatagc atactttttc ataaaacccc ttttaggcat aaaatataca
    56041 tctaatccat tttgcaattt ctcataataa acttcttctt tcaatatatc attaactatt
    56101 ttttccatag acatctcctc cattaatttc tcaagaagta tacagtatca agctctatat
    56161 tttttatagc ttcaactata tctttttttg taaccttttc tatactagat attatgtctt
    56221 gtacttcaga atttgtcttt gccatagact gagagaaaac aaaatcagac ataccaccta
    56281 tattatcagt tatagatttc atagaattaa ctaaagcaag tttactgttt tctaattctt
    56341 catcagatat atcacctgct tttacacttt ctacttgttt ttttatcaat tctacagctt
    56401 tatcataatc tttagtttct ataccagatg atataaacat gatactttta tacttctcta
    56461 ttgaagagaa tatatagtaa cataaacttt ctttttctct tacattaaca aataatttag
    56521 agtgtggacc accacctaag acattgctcc caactactaa agcataatat ttatctatgt
    56581 ctgcataatc cacatttgct ctataaccca taactaactt accttgagtt atttccattt
    56641 gttcatctat aacttttacc tcatccacat tttttataaa atctgctctt ggtatattta
    56701 taatttcttc tctttcaaat tttagatttt tacttattat atctacaact ttatcttcat
    56761 caaaattacc ttctacaact atatctatag gtgatgtttt aagtatgttt ttataatgtt
    56821 catataactc tcttgaagtt atactatcta tttcatcttc ataaccaaat tcacttacac
    56881 tatatctctc tcctttacac atagcctcaa tacatttatc ttgcgcatat tcctttttat
    56941 catttattat tgattgtatc ttttctctta aattctcttt ttcaatattt aaatattctt
    57001 ctttaaaccc accatctaca acaagcgtat tatttattac ttcattaaaa aattctacta
    57061 cttcttcaaa tatactctca tctaaatact tttcacttat gttaattact ttaaaactca
    57121 atacttgacg ttctccacgt tttacagcat cagctcccat agatgaacca tataaatcat
    57181 ctaatttatt ggatattgct ctagcactag gatatttagc gcttccactt gctattattg
    57241 atggtattag agcatttttt gtagtttctt tttcatccaa tagtctctga acatataaac
    57301 ttataagatt cgatttaaac ttttctgttt ttatcaaagt aagattaata ttattaccta
    57361 aatttattat ttttttagta ttaaccatat gtacctccta tttttattta aacatcaatt
    57421 ttaaaattga aatttattta ttttttaatt acatatactc acatataaaa accttctatt
    57481 taattatatc ctttttatta ttttctaaaa gcaatatttt tatttaaatt aaatacaaat
    57541 attatttaaa tcaatatgct ttaaagtcta gtttacaagc ttgatatata tgatgtataa
    57601 tcaatatgta tgtctataat taaaacaatt ttgcaatcct atagattgat tgtttaaagt
    57661 tatataaaga atataaaaaa gaataagaga gaggacgatt aatatgaaat taggtatagt
    57721 tggtttacca aatgtaggta aaagcacact atttaacgca ataactcaag caggtgcaga
    57781 atctgcaaat tacccttttt gtacaataga cccaaatgtg ggagttgtat ctgttccaga
    57841 tgaaagatta aacaaactac aagaattata taattctgaa aaaatagttc ctactgctat
    57901 agaattttgt gatatagcag gtttagttag aggagcttct aaaggagaag gtttaggaaa
    57961 taaattctta tcacatataa gagaagttga tgcaatagtt catgttgtta gatgttttga
    58021 ggatgaaaat gttgtacatg tagatggttc tgtagaccca cttcgtgata tagaaactat
    58081 aaacttagaa ttaattttct cagatataga gattcttgaa agaagaatta ataaaactca
    58141 aaaagctgct aaggctgata aaactttagg ttctgaactt gatttattaa aatcaataat
    58201 gtctacttta gaagaaagta aatgtgtaag aactatggaa tttacagaag atgaacaaac
    58261 attcgtaaat tcattggatt tacttacatc taagcctgta atatatgctt caaatgtatc
    58321 agaagatgat ttagctgata atggagaaaa taataaatat gttcaacaag ttaaagcatt
    58381 tgcagaaaca gaagatgccg aagttgttgt agtatgtgca caaatagaag cagaaatttc
    58441 tgaacttgat tctgctgaag aaaagaaaga gtttttagaa acattaggtc ttgaacaatc
    58501 agggcttgat aagcttataa aatcttctta tgctttactt ggtttaattt cattcttaac
    58561 tgctggtcca aaagaagtta gagcatggac tataaaagtt ggaagtaaag ctcctcaggc
    58621 aggaggaaaa atacattctg atatagaaag aggatttatc agagcagaaa caatagcctt
    58681 taatgatttg gttgagcatg gtactatggc tgctgctaaa gaaaaagggt tggttaaatt
    58741 agaaggtaaa gaatacatag ttaaagatgg agatgtaata ttatttagat ttaatgttta
    58801 aaatgattaa tcgtatacac tttgactatt atatataata actaaaaaac taagattata
    58861 ataatacaat attatcttat aataattata tagaatttaa aaataaaaga agagtatttc
    58921 aaatgatttg tataaattat tatgaaaaac tctttttttg atataataaa
//
LOCUS       DAYAOY010000013        57602 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYAOY010000013 DAYAOY010000000
VERSION     DAYAOY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.013"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     regulatory      141..226
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            388..909
                     /locus_tag="ACGI6B_000881"
     CDS             388..909
                     /locus_tag="ACGI6B_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9750346.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            1060..2736
                     /locus_tag="ACGI6B_000882"
     CDS             1060..2736
                     /locus_tag="ACGI6B_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFG9750347.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2748..3956
                     /locus_tag="ACGI6B_000883"
     CDS             2748..3956
                     /locus_tag="ACGI6B_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFG9750348.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            3971..5683
                     /locus_tag="ACGI6B_000884"
     CDS             3971..5683
                     /locus_tag="ACGI6B_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFG9750349.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            5676..6584
                     /gene="pilO"
                     /locus_tag="ACGI6B_000885"
     CDS             5676..6584
                     /gene="pilO"
                     /locus_tag="ACGI6B_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFG9750350.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6611..7177
                     /locus_tag="ACGI6B_000886"
     CDS             6611..7177
                     /locus_tag="ACGI6B_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9750351.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            7214..7741
                     /locus_tag="ACGI6B_000887"
     CDS             7214..7741
                     /locus_tag="ACGI6B_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9750352.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7762..9300
                     /locus_tag="ACGI6B_000888"
     CDS             7762..9300
                     /locus_tag="ACGI6B_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750353.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLYMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9400..10482
                     /locus_tag="ACGI6B_000889"
     CDS             9400..10482
                     /locus_tag="ACGI6B_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFG9750354.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10463..11245
                     /locus_tag="ACGI6B_000890"
     CDS             10463..11245
                     /locus_tag="ACGI6B_000890"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFG9750355.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            11527..11997
                     /locus_tag="ACGI6B_000891"
     CDS             11527..11997
                     /locus_tag="ACGI6B_000891"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFG9750356.1"
                     /translation="MVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNIVQ
                     FDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFILA
                     FLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            12011..12571
                     /gene="pth"
                     /locus_tag="ACGI6B_000892"
     CDS             12011..12571
                     /gene="pth"
                     /locus_tag="ACGI6B_000892"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFG9750357.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            12588..15974
                     /gene="mfd"
                     /locus_tag="ACGI6B_000893"
     CDS             12588..15974
                     /gene="mfd"
                     /locus_tag="ACGI6B_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFG9750358.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            16005..17000
                     /locus_tag="ACGI6B_000894"
     CDS             16005..17000
                     /locus_tag="ACGI6B_000894"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9750359.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            17124..17663
                     /gene="spoVT"
                     /locus_tag="ACGI6B_000895"
     CDS             17124..17663
                     /gene="spoVT"
                     /locus_tag="ACGI6B_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HFG9750360.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            17783..19396
                     /locus_tag="ACGI6B_000896"
     CDS             17783..19396
                     /locus_tag="ACGI6B_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFG9750361.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19503..20951
                     /gene="mazG"
                     /locus_tag="ACGI6B_000897"
     CDS             19503..20951
                     /gene="mazG"
                     /locus_tag="ACGI6B_000897"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFG9750362.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            21040..21318
                     /locus_tag="ACGI6B_000898"
     CDS             21040..21318
                     /locus_tag="ACGI6B_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFG9750363.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21390..21632
                     /locus_tag="ACGI6B_000899"
     CDS             21390..21632
                     /locus_tag="ACGI6B_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFG9750364.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            21694..21948
                     /locus_tag="ACGI6B_000900"
     CDS             21694..21948
                     /locus_tag="ACGI6B_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFG9750365.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            21949..22482
                     /gene="yabQ"
                     /locus_tag="ACGI6B_000901"
     CDS             21949..22482
                     /gene="yabQ"
                     /locus_tag="ACGI6B_000901"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HFG9750366.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            22488..22772
                     /locus_tag="ACGI6B_000902"
     CDS             22488..22772
                     /locus_tag="ACGI6B_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFG9750367.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            22885..23805
                     /locus_tag="ACGI6B_000903"
     CDS             22885..23805
                     /locus_tag="ACGI6B_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFG9750368.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            23895..26267
                     /gene="spoIIE"
                     /locus_tag="ACGI6B_000904"
     CDS             23895..26267
                     /gene="spoIIE"
                     /locus_tag="ACGI6B_000904"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HFG9750369.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            26618..28510
                     /gene="pepF"
                     /locus_tag="ACGI6B_000905"
     CDS             26618..28510
                     /gene="pepF"
                     /locus_tag="ACGI6B_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFG9750370.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            28624..29589
                     /gene="galU"
                     /locus_tag="ACGI6B_000906"
     CDS             28624..29589
                     /gene="galU"
                     /locus_tag="ACGI6B_000906"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFG9750371.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            30089..31693
                     /gene="rho"
                     /locus_tag="ACGI6B_000907"
     CDS             30089..31693
                     /gene="rho"
                     /locus_tag="ACGI6B_000907"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFG9750372.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            31846..32046
                     /gene="rpmE"
                     /locus_tag="ACGI6B_000908"
     CDS             31846..32046
                     /gene="rpmE"
                     /locus_tag="ACGI6B_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFG9750373.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            32191..33084
                     /locus_tag="ACGI6B_000909"
     CDS             32191..33084
                     /locus_tag="ACGI6B_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HFG9750374.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            33102..33950
                     /gene="prmC"
                     /locus_tag="ACGI6B_000910"
     CDS             33102..33950
                     /gene="prmC"
                     /locus_tag="ACGI6B_000910"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFG9750375.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            33994..35058
                     /gene="prfA"
                     /locus_tag="ACGI6B_000911"
     CDS             33994..35058
                     /gene="prfA"
                     /locus_tag="ACGI6B_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFG9750376.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            35187..35867
                     /locus_tag="ACGI6B_000912"
     CDS             35187..35867
                     /locus_tag="ACGI6B_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFG9750377.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            35896..36936
                     /locus_tag="ACGI6B_000913"
     CDS             35896..36936
                     /locus_tag="ACGI6B_000913"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFG9750378.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            36953..37402
                     /locus_tag="ACGI6B_000914"
     CDS             36953..37402
                     /locus_tag="ACGI6B_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HFG9750379.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            37419..37871
                     /gene="rpiB"
                     /locus_tag="ACGI6B_000915"
     CDS             37419..37871
                     /gene="rpiB"
                     /locus_tag="ACGI6B_000915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFG9750380.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            37907..38536
                     /gene="upp"
                     /locus_tag="ACGI6B_000916"
     CDS             37907..38536
                     /gene="upp"
                     /locus_tag="ACGI6B_000916"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFG9750381.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            38831..39646
                     /gene="yqeB"
                     /locus_tag="ACGI6B_000917"
     CDS             38831..39646
                     /gene="yqeB"
                     /locus_tag="ACGI6B_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFG9750382.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            39872..40309
                     /locus_tag="ACGI6B_000918"
     CDS             39872..40309
                     /locus_tag="ACGI6B_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFG9750383.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            40494..40721
                     /locus_tag="ACGI6B_000919"
     CDS             40494..40721
                     /locus_tag="ACGI6B_000919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HFG9750384.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            40726..41112
                     /locus_tag="ACGI6B_000920"
     CDS             40726..41112
                     /locus_tag="ACGI6B_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFG9750385.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            41115..41819
                     /gene="atpB"
                     /locus_tag="ACGI6B_000921"
     CDS             41115..41819
                     /gene="atpB"
                     /locus_tag="ACGI6B_000921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFG9750386.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            41888..42148
                     /gene="atpE"
                     /locus_tag="ACGI6B_000922"
     CDS             41888..42148
                     /gene="atpE"
                     /locus_tag="ACGI6B_000922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFG9750387.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            42271..42768
                     /gene="atpF"
                     /locus_tag="ACGI6B_000923"
     CDS             42271..42768
                     /gene="atpF"
                     /locus_tag="ACGI6B_000923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFG9750388.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            42765..43310
                     /locus_tag="ACGI6B_000924"
     CDS             42765..43310
                     /locus_tag="ACGI6B_000924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFG9750389.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            43327..44829
                     /gene="atpA"
                     /locus_tag="ACGI6B_000925"
     CDS             43327..44829
                     /gene="atpA"
                     /locus_tag="ACGI6B_000925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFG9750390.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            44857..45735
                     /gene="atpG"
                     /locus_tag="ACGI6B_000926"
     CDS             44857..45735
                     /gene="atpG"
                     /locus_tag="ACGI6B_000926"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HFG9750391.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            45748..47142
                     /gene="atpD"
                     /locus_tag="ACGI6B_000927"
     CDS             45748..47142
                     /gene="atpD"
                     /locus_tag="ACGI6B_000927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFG9750392.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            47145..47405
                     /gene="atpC"
                     /locus_tag="ACGI6B_000928"
     CDS             47145..47405
                     /gene="atpC"
                     /locus_tag="ACGI6B_000928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HFG9750393.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            47867..48247
                     /gene="acpS"
                     /locus_tag="ACGI6B_000929"
     CDS             47867..48247
                     /gene="acpS"
                     /locus_tag="ACGI6B_000929"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFG9750394.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            48262..48723
                     /locus_tag="ACGI6B_000930"
     CDS             48262..48723
                     /locus_tag="ACGI6B_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFG9750395.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            48744..49334
                     /gene="gerS"
                     /locus_tag="ACGI6B_000931"
     CDS             48744..49334
                     /gene="gerS"
                     /locus_tag="ACGI6B_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HFG9750396.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            49349..50506
                     /gene="alr"
                     /locus_tag="ACGI6B_000932"
     CDS             49349..50506
                     /gene="alr"
                     /locus_tag="ACGI6B_000932"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFG9750397.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            50539..50826
                     /locus_tag="ACGI6B_000933"
     CDS             50539..50826
                     /locus_tag="ACGI6B_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFG9750398.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            50795..51178
                     /locus_tag="ACGI6B_000934"
     CDS             50795..51178
                     /locus_tag="ACGI6B_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFG9750399.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            51349..52176
                     /locus_tag="ACGI6B_000935"
     CDS             51349..52176
                     /locus_tag="ACGI6B_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFG9750400.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            52169..53110
                     /locus_tag="ACGI6B_000936"
     CDS             52169..53110
                     /locus_tag="ACGI6B_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFG9750401.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            53533..54627
                     /locus_tag="ACGI6B_000937"
     CDS             53533..54627
                     /locus_tag="ACGI6B_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750402.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            54853..55269
                     /locus_tag="ACGI6B_000938"
     CDS             54853..55269
                     /locus_tag="ACGI6B_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750403.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(55451..56020)
                     /locus_tag="ACGI6B_000939"
     CDS             complement(55451..56020)
                     /locus_tag="ACGI6B_000939"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFG9750404.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            56174..57331
                     /locus_tag="ACGI6B_000940"
     CDS             56174..57331
                     /locus_tag="ACGI6B_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFG9750405.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        23134 a         6125 c        10742 g        17601 t
ORIGIN      
        1 cataattttt ctagaaattc tgtttcaata tgtaaaaagt tggtatatat aaatagtata
       61 aacagtgttg ctaaaatatg gtagatatta tataatataa gtggatatgt atatacatta
      121 tgagttatat aatttgaatt taataaaata gaaacgttga tttatgttct gtaatgtggg
      181 caccttggag catattgagt tagtggtgca accggctatg aaattgtatt tatttataaa
      241 tactattatt ttcatagctt tttttattta aaaaatggtt atatgttaga aataaatttg
      301 gttagtacgt ttaaataatt ttacttagat ttttaaccag atatattgta agtaagtatt
      361 aattaaaaaa tttaaggggg aataaaaatg aagttaaaaa agaataaaaa aggtttcact
      421 ttagtggaat tattggtagt aattgcaatt ataggtatat tagcagtagt ggcagttcca
      481 gctttattta gtaatataaa caaggctaag gtagcaagtg ttgagtctga ttatagttca
      541 gttaagagtg cagcattatc ttattattca gatactaata agataccagt tacaccagat
      601 ggtcaaactg gtttaagtgt tttagagact tatatggagt ctctgcctga taaagctgat
      661 ataggtggaa aatataaatt gattaaagtt ggtaataaat tggtattaca gataggtaca
      721 aatactgaag gagttacatt aacagaggca caatcagcaa agttattgag tgatataggt
      781 gaaaataaaa tatatacaag cgctacaaca aatagtttgg gaaatccatt aacaagtaat
      841 acaaaaatag ataataacgt tctatatata gtacttatag ataatactgt gatggacaca
      901 acaaaatagt taaaacaata tttttataaa aatattgtaa atttaatgaa aaatataata
      961 taaaagacag gaagaaaatc ttcctgtttt tttatactat tttctttaat gttcaattat
     1021 gaatgatgat ataattgaga tagtaaagga gtgaaacctg tggctaaaaa agttagaata
     1081 ggagacaaac tggtcgaaaa aggatatata acagaagaac agcttaaatg ggcactatca
     1141 gaacaaaaaa acagtggaaa aagactagga gaatttctag tacaagaagg gcttatagat
     1201 agtaacttac taataagtgt attaaaagaa ctcttggata tagaaagtat atttttagaa
     1261 gggacagaaa tagacacttt agctacaaaa atggttccag aaaatatatg caaaagatat
     1321 actgtatttc catttaaaat tgatggaaat aaaatatgtc tagccatgtc agacccacaa
     1381 gatagagaag cagttcaaga tgtaaggcgt atgtctggta aagacgtaga gatatttata
     1441 tcaagtacag aagatataaa caaagcaata ggtcatgctt atgcacattc agaaataaat
     1501 aaggcaatga ctgagtacaa caaaaataga actggtggag taagagaaac agtaatactt
     1561 gaagaagatg taaatgctgc tccaatagta cgattagtaa ataacatact agaaaatgct
     1621 gttagaatgg aagcaagtga tatacatata gaacaaagtg aaaattacat gcgtgttagg
     1681 tttaggatag atggaatgtt aagagaatat atgaggatga actcagcacc atataaggca
     1741 gtaatcagcc gtatcaagat aatgtcagat ataaatatat ctgaaaagag aataccacag
     1801 gatggtagaa tctatttaaa agtagataat aaacccatag attttagggt ttctacaatg
     1861 ccaactaaca gggatgaaaa gatagctatg agagttcttg ataagagtaa ttttatggtt
     1921 agtaaagaag tactaggaat agacgaacat ggctctaaga tatatgatga attgataaac
     1981 actccttatg gtcttatatt ggttgttggc cctactggaa gtggtaaaac cactacattg
     2041 tattcaatgt taaatcaact aaatacagaa aatagaaatc tattaacaat agaagaccct
     2101 atagagtatg aattacctgg tgtaaatcaa tctcaaataa atgaaaaggc agggcttact
     2161 ttcgccagcg gacttagagc ttttatgcgt caagacccag atataataat ggttggagag
     2221 ataagagata ctgagacagc agaaatagca attagagctt ctcttacagg acatttagtt
     2281 ttaagtacat tacatgcaaa tacagcagtt ggagcaattt cacgtctttt agatatggat
     2341 gtggaaagtt ttttaataac ttcatctgtt ttaggtgtaa tatctcaaag acttacacga
     2401 aaaatatgtg agcattgtaa ggtttcatat gaagcagata ttggagaaaa aaaggctctt
     2461 ggtattgatg taaatgaatc agtaactatt tatagaggaa aaggttgtga aaggtgtaat
     2521 aatactggat ataaaggacg tctaggtata tttgaaatgc ttgaaattac tccagaaata
     2581 aaagagttaa tagattcatc tgcaaatcaa agagaaattt taaagatggc tagaaaacaa
     2641 ggtatggtta gcttaaaaga ggatatagtt aaaaaagttt taaatggaaa gacaacagta
     2701 gaagaaatga ttagaataat tttgatgact gattaaagga tgatattatg aaaagtttta
     2761 aatacacagt aataaaagag gacggaaaaa agcaaactga tgtgattgaa gccaacgatt
     2821 ttaatgaagc tagaaatctt cttagaaaga gaaatcttag agttattgaa atcaaagaaa
     2881 gcaaaaataa aaatataaga ttatctagta gaaagagaaa aaaagactta ggagctgacc
     2941 agataagcca cttttgtaga cagtttgcta ttatagtatc ttctggtata aatagtattt
     3001 ctggattaga aacattagct agaaggtcaa ctaacattac attaagagaa gaaattaatc
     3061 gtatagtggc agaaataaaa gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat
     3121 caaagtttcc aaagttactt ggtgctatgg tagctacagg tgaagctaca ggtaaattag
     3181 atgaagtatt aaaaagtatg gcttcatttt atgcaagtga acacagggtt aaacaaaagc
     3241 ttagaaatgc tgcaacatat ccattaatag ttttgatttc ttcatttata atgattttta
     3301 tatttacaac atttatggtg cctaagatga ttaattcaat tacaacagta ggagcaactc
     3361 ttccacttat aacaagaatt gttatggggt ttggtatgtt tatgaagaag ttttggataa
     3421 tagtactttt agttattata ttatttatct atcagtttaa aaaatatcta aaaacgtcag
     3481 ttggaagggc tcataaagat agagttataa ataagatacc tgttttagga aaaggaataa
     3541 attgtatggt agctacacga ttttcaagag cattgtatct atttgtatca acaggatacc
     3601 cattagttca agggcttgat tatattatag atagtgtaaa taacaccatg gcagagaaga
     3661 ttttagcatc agctaaagat ggaataacaa gaggagaagg cttagcagaa aatttagaga
     3721 gatatacgta ttttgactct gtgttggtac agatgatagc tataggggag cagacagggg
     3781 aattagagaa tataagtaga cagatggcag aattttatga gtatgaatcc gagatttatt
     3841 taaataggat ggcctcaatg atagagcctg ttttaataat tgcagttggt attatagttg
     3901 cgatactggt tgtatcaata ttcatgccaa tgcttagtat atatgatgcc atgtaggagg
     3961 gaaaggacaa atgaacaagg tttatgtatc atattatggc gattctattt ccatagttga
     4021 gggcagatat aaacacgata aggataagtt ttatgtaaaa aattttaatg tgttttcaat
     4081 agaggatatt gcacaagatt tttctaagga aaagtatgct ctattgagat acgcattaga
     4141 aaatgttaag ttaaaatcaa gacatgtagt tttttgttta aatacaagag atgtaatttt
     4201 aaaaccccaa aaacttccta atatggacaa aaaggattta gacggattta tgaagctaga
     4261 gatggacgaa atgatggctt tggagtcaga tgaatatgta ttttcgtatg aagtatctag
     4321 tgaaacacct gataattcag atggcgatga atcaagttta aatcttatta ttggagctat
     4381 acaaaaggaa gaggtaaatg caattgtaga gataatgcat gaatttgatt tagtattaga
     4441 tagaatagat acattatcaa cagcttactt aagaatgctt aaaaatttag actacaatga
     4501 tataatggta accaatataa gtgacaatag cacaattata aatatatata agaaagatac
     4561 tttatttatt agtgataata tacctataaa gataactgct aaagatatgg acatgcaatt
     4621 attaaatgta gtagaagaat caaaaggtct tatgaattac tattcgtcaa gaaactttgg
     4681 taaggttacg gatactatat taatattagg aaaaagatat tccaataaat taatttatga
     4741 aacttttagc tctgcatttt caagctatgt ttctcaaggc cttactgaga taatggatat
     4801 atctgatatg ataactggag atatagatga agatgatata aattcaattg tagagctttt
     4861 aggaagtatg atagaaccta aagggaaaag agattttgaa aatataaact ttttaccaat
     4921 ggatatactt agaaaacaga taaaagctaa acaaatgaaa aacatactga aaattgctcc
     4981 aatagttatt ttgattcttg ctatacctta tttatgttta attttttcaa ataatttagc
     5041 aaataaggaa cttgaaaatg ttaatggtga aataagaaat attgaatctg catattatca
     5101 gattggaaat atagaaaaaa tgatacagag taaaagcgaa gaaataaagg tatatgatat
     5161 gttaattggg aaaaaggtaa agtgggaaaa tttattggat gatatagata aaaatactcc
     5221 aagtaaagtt gaactaacaa gtttatcaac aacttatcaa aatcttggaa ctaataataa
     5281 agctgataat tcaaaaagta ataagactac aacagataaa tcaagtaaca atgaaacaaa
     5341 atcaagtacg aaaagtgata gtaatggaga taaaacagat aatgatttaa atgaagataa
     5401 gactacaaat gtgtcagaag cattaactgg aaatagtgag actaatagta ataacacaga
     5461 taaagatgat aaaactccat tgtatgataa gataccaaat gttataaata tagaaggaaa
     5521 tgctaaagat tcctcttatg taggtcaatt tttatataaa ttaaagggat tatactattt
     5581 caaggatgtt aaattacata gcataaaaag taatgcagaa gattctaact atacatttag
     5641 tatgacatta tacctgaaag aaggtgtatt aactaatgaa taaagatata tttaaaaaag
     5701 atataaaaga tatatttaaa atggaagtta gtctaaaagg gattcttata aaggttataa
     5761 ttttattagc tattttatca tatggttatt ttggacttta ccctcaatat cagaaatata
     5821 ttgctacaaa aaattctgta aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa
     5881 ctgcacttat gccaaaatat gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa
     5941 aaagcaaaaa tcttttatat gatatgcaag atggtctttt tttaataggt ttagataaaa
     6001 aaatgaaggc ttataatatt gacttaacca gttatgacgt tggaaatgtt aaagaatacg
     6061 aacattttta tggtatatca acaacaataa gtgttagagg tgattataga aatgtcaaaa
     6121 aattaatggc ttatttagaa gaacaaaaaa atgttactca aatactagat tatacaatga
     6181 cttcatatat agaaccagaa aaagaagagg aagaaacaac aaacaataag gatttaatta
     6241 aaaatgtgaa cataaataaa gatacaaaaa tatactgggt ggaaggcgaa aataagtggc
     6301 atttatataa ggattgtcca ttgataaaag agggagagat tctaaaaaat gacaaacttt
     6361 tagataggtc tactaattat ctatgtaata cttgtgtact tagagctgca acagaaaaag
     6421 aagcaaaaaa agaagtcgta gctccaaagg cagaaggtgt agtagatgct acatttaaat
     6481 ttataatgta cactaaagag aatactacaa tgatacttga tacagataat ccatcttcat
     6541 ggagaccagg aaaatataat ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta
     6601 aagataggtg atgagtttgt acaaaaataa tgaaaaaggt cttactttat tagaagtaat
     6661 aatagctgtt tttatactga caatagtttt aagtatttct tataaagtgt tcaatggtat
     6721 aacatcagca gtaaaaaagc aacagattat tacagatgct caagtaaata ttaatctaat
     6781 taataagtat ctaaatagag atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg
     6841 taataattat gaatacaata tagagatgcc agataatgtt gtaaaatatg aagtttctat
     6901 agaaactaaa aaaaatacag aggtatattc tgtaacgaga atccaaaaca atacaattga
     6961 tacagaaaat gaagttagag aagagataat ctataatcaa ccactagttc aaaacaataa
     7021 agaaatgaag gaaacaccat ttaaaataga aaagcaaact ggtaaatcta tttatactgt
     7081 aagtatatac tataatgaat cagtgcaaga aagtcataaa aatagcaatt taaataacaa
     7141 aacttataca tttgatgtta tgtcaagaat aggataataa gaatttatta aaaaataagt
     7201 aggttgataa aatatgagta aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt
     7261 agtagctatg gctattatgg gaattgtact atttgcattt tttaatatta tcaatactaa
     7321 taacaaagca aacactaaaa atgacacaga tataacttcc ttaaactatg tccaaagtga
     7381 aatagaaaat ctaagagaaa agataaaaag tggagaattt gattttgata gtttagataa
     7441 actagaagat ggaactgtcg tatatgaaaa attaatagat aaatcaaaaa aagttgtata
     7501 tgataaagtt cttagtgaag gtgatgtgag cttatatgat actccatatg aaaagattac
     7561 aacaataaaa gatgaagatg gtaatttaat cgacaaagga aatattacaa ataagataaa
     7621 gactattgta gaagataaaa gtggtcagat atataaaata gctgtaactg ggaaaagcat
     7681 gaatgactat tcaagtaaaa aagaggttaa aattgtaact gaaatattta aagataaata
     7741 attttacttg ggggtgagat tttgaggaag tggaataaat ttaaaagtga aagaggggca
     7801 gctttagttt tagttttggt tgtagtagca ttgctttcta ttgttggatt gatattttca
     7861 aatcagatag ctaatagaat aaaatctaca aagactacta atgaaggtat acaagcaaag
     7921 tatttagcag agacatgtgt ggaaaatagt attgataaag catatgaaaa attgtatgat
     7981 gagcttgaga aaatggataa tgagtttaaa agtgaaaatc aagaaaaatc catatctagg
     8041 tcaaaattaa gagatatatc agatgaagat tttaataatc aagatgagga aaatattgaa
     8101 gcagaaagac ttggatatat gaataatata aatttctatt taaataaagc ttcttcagac
     8161 ttggaaaaag ctagtatgga attgaaaaag ctatatgatt tatatatgtt ggactataga
     8221 gacattgaat atgtagatgc aaatattatt tcacatagag attctatctt agaaatttgc
     8281 aagaattata cttctggaga tattagtaaa ataaatgaat atattctgaa agaagatata
     8341 gactcaacta cattgattga ggcaaagtta gtgaataatg atattctttt aaaaatgttt
     8401 ttagaagaaa ataaaattga aaatgaacat ttaaattcag ccttttcaca tacttataag
     8461 gcattagata acatttcttt ggcgatgcaa aatatgattg agtatagaca tacgtttcat
     8521 atagatgagc caaaagtaga agtatctaat gggattccag attctcaaca atattatgaa
     8581 ctaatacaaa atcctataat aaatagtatg gagtatatct ggaatagtaa atgggataca
     8641 ttagaaaaac tattggaaat tttaccaaat caaactcaag gatttaattc tttaagagtt
     8701 catttgagaa ataatgtaag aaaatttgag aagttaagcg ataatatatc tagtggtaaa
     8761 aaaaatacag caaaaaattt cttaaagtat aaagagttat tgtatgaaat atcagaccaa
     8821 tgtaatcaac taaaaagtat gtcttatgag aaaatacctg ttaaatatga taacatggct
     8881 ttgataacaa cttttgacta tatacaaaat gagcttttag cagagataaa atgcagatta
     8941 aaagaattaa aaccacaaga aattgataaa acagaaggta ttactataaa aatacctttt
     9001 tataaggctg actacgacat gacaaaagaa ggatggccta aattaaaaga aaatggttca
     9061 ggggcggagt taagcttaat ggtaactggt gataaagatg gaataaaaga agtagaagtt
     9121 acagatggta aaaaaaatat tattggttta ggtgttgaag aaaattcaaa ttcaaagtat
     9181 aaggttgacg ccattgtaaa ctttaatcta aatatagata caaatgtagt tggaaattat
     9241 gatataaaag ataaaatttt gattaatcat gatataagtt catacaaaaa agtaaactaa
     9301 atataagaag caacttagta taaataaata ataaaataat tttattattt actactaaaa
     9361 aaataagtaa actaatagaa gatatggaga gtaaatatta tgtatattta tgatttatta
     9421 gagcaaggta taagactaaa cgcatcagat atacacataa cagttggaac aaatcctgta
     9481 gctagagtaa aaggtggatt tgtaaaacta agtgaacaaa tattgacatc agaagtaaca
     9541 atgcaaatgg caaaagacat agcaggggaa tctatgttta aagtaataga agaacatgga
     9601 gaagctgatt tttcagcttc tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg
     9661 caaaaaggaa attacgcaat agcaataagg actataacag cagaaattcc aacatttgaa
     9721 aaattaggcc ttccagagag tataaaatct tttactgaaa agcataaagg attagtttta
     9781 gttacaggtc caacaggaag tggtaaaagt acaacacttg caagtatgat aaatataata
     9841 aatgaaaaac aacaaaaaca tataattaca cttgaagacc cgatagaata tgtacatcat
     9901 cataaacaaa gtttagtaaa tcaaagagaa gtaggaactg atacagaaag ttttcattca
     9961 gcccttagag ctattttgcg tcaggaccca gatgtaatac taataggaga gatgagagac
    10021 cctgaaactg tttcaatagc attaacagca gcagaaacag gtcatttagt attttcaaca
    10081 ctacatacag tgggggcagc taagacaatt gatagaatag tagacatgtt tcaaccatcc
    10141 caacagcaac aaataaaaac acagttatct acagtttgtg aaggtgtggt atcacagcag
    10201 ttacttccta cagcagatgg gaaaggcaga atagcagcaa ttgaattgat gtttgctact
    10261 ccagcaataa aaaacctgat aagggaaggc aaaacatatc aaattccaaa catgatacaa
    10321 actggagtta aagtaggtat gaaaactatg gaccaagatt taatggaact ttataaaaat
    10381 ggaaagatta caaaagatat ggcacttagt aggtgtactg accaagagtt tatgacaaga
    10441 atgataggtg gagtaaacta caatggatat tataatcgtt aatctatatg tatttatggt
    10501 gggtatagtg tttggaagtt tctttaacgt ctgtattttt aggattccag aaggtatatc
    10561 tatttctaga cctccttctc attgtatgag ttgtaatact agactaaagc caatagactt
    10621 aataccagtg ttaagctatt taatgagtgg cagaaagtgt agatattgtt atgagaaaat
    10681 ttctagtaga tatgctatag tggaattaat tacaggactt ttatttttaa cagtatttat
    10741 gttatatgga atatctacat caactattta ttatttggtt cttgtgtctt tactgattat
    10801 aattacattt atagatattg accactttat aataccagat aaaatattgg tttttggagc
    10861 tatattttct atgattttta accttttatt taaagaaatt ccagttaagg attcaattct
    10921 aggtgttttt atttgtggag gaagtgtctg gattatagtt cttttgattg aatttgtaat
    10981 aaaaaaagag tgtatgggtg gaggagatat caaactattt gcaatgctag gtttttacat
    11041 gggtgttaaa aatggtcttt tgacagcctt actaagcgtt tatgtgggag cagtctatgg
    11101 aatctgtgta ataattataa gtagaataaa aggaaaggaa tataattctg ttataccata
    11161 tggacctttt atatctattg gagcattaat aacaatctta tgtggaaagc aattacttga
    11221 actttatttt tctatggtta tatagatgat agtcaagtta aataaagttt taatttaaaa
    11281 aaatgaatta aagtatttaa taaggacaat attattatta accctattac taaagtgcta
    11341 caaactatgg agtatataat aataatttcc ttattttttg cacatttgat aaataaatct
    11401 ctatgtgttt tttgtgatag agaaaagaaa aatttataca atacgttttt tatttacata
    11461 ataactgtag ttttatctgt attaatatat ctaaaatttg ggattagtgt agaatgtgta
    11521 caatatatgg ttttaattcc atttttaata attatatcca ttgtagatta ctacacaata
    11581 tatgtatatg atataacaat agttagtggt ataatagttc aaggtataat ttttttggtc
    11641 tttttcaata tagttcaatt tgattatata aatcatatat ttggactatt gataggcttt
    11701 attatacctt atattattgc taaactaaca aaaggattag gctatggaga tgtaggtctc
    11761 tatagcttat gttgctttgc attgggaaat aattatgggt tttatgtgat agttttatca
    11821 tttattttag cgtttttgtt ttttattatt ttatacataa ctaaaatatt taaagatgta
    11881 gatataagaa agcaaattcc ttttacacct tttatttcct tgtcaacaat tttaataata
    11941 tttacagagt ataatatatt taatttttat tataatttgt tatacaaatt tatataacag
    12001 gaggtaagaa atgtacgtag tagtagggtt aggtaatcca ggaaagaagt atgagaagac
    12061 tagacataac gttggatttg atgttataga tattcttgct aaagaataca atattagtgt
    12121 tactaaaata aagcataaag ctttgattgg tgaagggaga gtaggtactg aaaaagtatt
    12181 attagttaaa ccacaaactt atatgaattt aagtggagaa acattaatag atatatataa
    12241 atattataag gttgatttaa gtaatatagt agtagtatat gatgatatag atttagaagt
    12301 tggaaagata cgtataagaa aaaaaggaag tggaggaact cataatggca tgaaatctat
    12361 aactaagtgc ctaggttcaa atgactttcc aagagtgaga gtaggtgtat ctaaaccaga
    12421 agctggtcaa gacttagcag actttgtact ttctagattt agaaaagaag aatctgataa
    12481 cataaatgaa gcattagaaa aagcggcaga tgctattgac tcaataataa gagaaaatat
    12541 agatatgtct atgaacaaat ataatggata gtgagaggga gatttaaatg aatgatgttt
    12601 ttttatatcc tttgcaaaat tcaaaagagt ataaagatat aataaactgt ataaaaaata
    12661 caaaaggctc attattggtt aatggacttt taccagtaca aaaacctcat atatcgtatt
    12721 ctatatttaa tgatttatca cgacaaatga tttttataac aagtagtgat ttagaagcaa
    12781 agaaagtata cgaagattta agtttttata tggaagataa agttgaatat ttaggttttc
    12841 aagatattta tttttatcac ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa
    12901 aagtattgtt aaaactagca aataaagaaa aaatcatttt agtaacttct attgaggcag
    12961 ttttaagaaa atacatacca aaacaagttt tgctcgatag tgtatctcat tataaggtgg
    13021 gagatagctt agacttagaa aagttaactg agaaattagt ttcattggga tatgaaagag
    13081 tttcaaaaat agaggggttt ggtcaattta gtataagagg tggaattatt gatgtatttt
    13141 cacttgaata tacaaatcca attcgtatgg aattatttga tgatgagata gattccatac
    13201 gaacttttga cgtatattca caaaaatcaa ttgataaatt gcaacaattc tctataactc
    13261 catctaggga atttatttat cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag
    13321 aaacaactaa aaatactgat gaaggtacat ttcaaaatat agattatata tcaagtaaga
    13381 cttattttga aggtgtagag aactatatag attatattta tccagaagaa aacaaaagta
    13441 tatttactta tttagcagat gatgcaatag tatttattaa tgatataacg agacttaaag
    13501 aaagatgcga aaattatata aatgagttta gagaaaatta taaattaaat cttgaaagag
    13561 gtctggctat taaaaaccaa ggtaaattgt tatatcacta tacagattta gagtacttag
    13621 taaaagacaa gtctgtggta ttaaactcgc tattgccaaa gtctataaat aacttttcta
    13681 taaaatctat aataaatttt gaaagtagag aagttcctac atttaatgga aaggtagatt
    13741 tgcttgtaga agaattaaat cggctgaaat ataatggata caaaataatt ttagcaacaa
    13801 ataccttaga aagagcaaat aaattaggaa aagatttatt ggacaaaggc ttagaaacga
    13861 ccatttctaa agatagagat attgaaatta aatcttcaca agttataatt gtacctgcac
    13921 acataaacag tggttttcag tataaatcta taaagtttgt tgttataaca gataacgaaa
    13981 tgataggtgt ttataaaaga gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga
    14041 aaatagaatc ctttttagat ttaagtgttg gagattatgt agtccatgaa aatagtggtg
    14101 taggtagata cacaggaatt gaacaaatta cagtcaatgc tattaaaaaa gattatatga
    14161 aaatagtata tcaaggtgga gataatctat atgtgccaat agaccagatg gacaaagtac
    14221 aaaaatatat tggtgcagaa gtagaaaaag ttaagttaaa taagcttgga acaaatgagt
    14281 ggacgaaagc aaaagcaaaa gttaaaaaag aaattgaaga tatgacaaaa gacttgattg
    14341 agctttatgc aaaacgtgaa aaaatacaag ggtataagtt ttcaaaagat acaccttggc
    14401 aggcagagtt tgaatcgttg tttccatatc aagaaacaga agaccaatta aaggctatag
    14461 aagaaactaa aaaagatatg gaatcaagta aggtaatgga taggctagta tgtggagatg
    14521 ttggttatgg aaaaacagag gtagctatta gaagtatttt taaagcttgt atggaccaaa
    14581 aacaagttgc tgtattggtt ccaacaacaa tattggccca acaacattat aatacattca
    14641 aagagaggtt tgaaaattat cctttgagag tagaagtttt aagtaggttt aaaacaccaa
    14701 aacaacaaaa acagattatt gaagatgcta aaaaaggatt agtagatatt ttaattggaa
    14761 cacatagaat aatatccaaa gatataaatt taccaaactt aggtcttgta gtcattgatg
    14821 aagaacaaag atttggagta aaacataaag aagctttaaa gaaaattaaa tctacagtag
    14881 atgtacttac actttcagca actcctatac caagaacatt acatatgtct ttaagtggaa
    14941 taagagacat gagtgttata gaagaacctc cacaagaaag acatcctgta ataacatatg
    15001 ttacagagag taaggaaagc gtaattcaag atgagataga gagagaatta agtagaggtg
    15061 gacaggtatt ttttgtatac aatagagttg aacacattga agaaatggca agtatgattc
    15121 aaaaactcgt accagatgct agagtggctg ttgcacatgg aagaatgact tctaaatcat
    15181 tagaaaatat aatcctagga tttttaaata aagattatga tgtactagtc tgtactacaa
    15241 taattgaaac tggtatggac atatcaaatg caaatacaat gattatatat gatgcagata
    15301 aaatgggatt ggctcaactt taccaattga gaggtagggt tggaagaagt tcaagacaag
    15361 gatatgcata tcttttgtat gaaaaagata aaactttaag tgagatagcg gagaagagat
    15421 taaaagctat cagagaattt acagagtttg gttctggttt taaaatagca atgagagacc
    15481 ttgaaataag aggagcagga aatattttag gttctcaaca acatggtcat atggcagtta
    15541 taggttatga tttgtacgtt aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc
    15601 ctattgtgga ggaaattgat gttgaaatag atttaagtgt aaatgcatac attccagata
    15661 attatatcaa agatgagttg attaaaattg aaatgtataa gaaaatagct tctattgaaa
    15721 acaaagaaga catgttggat atacaagaag aacttgagga tagattctct gatataccta
    15781 aaccagtcca gacacttctt acaatagctt atataaaatc gttatgtaag atattaaaaa
    15841 tagaaaagat aagacaatta aaagatgaaa tattattagt tccaataaca aagtatagaa
    15901 ctaaacagaa aataggttat aatatagtta cagaattaga agaattatta gaaaagatgt
    15961 gcaaggtaaa ataacttgta aatttaagaa gggatgtttt aaatttgaaa aaagtaataa
    16021 cattggtaat agctatgatt ttggtagtat ctgtgactgc ttgcagttct agtaaaggag
    16081 aaactgtggc aacagttgaa ggtacaaaga tatcatcaga tgaatttaaa aaaactatag
    16141 ctctttataa agattctatg gaacaaactt atggaaaaga tatatgggat aaggaagttg
    16201 aaaagggtgt aaaatacaag gacaagttta aagatttaat tcttgaccaa cttataacta
    16261 cagaagtaat atattctcaa gctaaaaaag ataacttact tcctaaaaaa gaggatgttg
    16321 aaaaaagttt taaagaatta aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac
    16381 ttaagaaatt aggtatagat gatgagtttt taagagacca acaagagaaa gatttagcaa
    16441 tgcaaaacta tcaaagtaac tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa
    16501 aatactatga tacacataaa gatgaattta aaaaagatga ggtagaagct tctcatattt
    16561 tattgaaaac agtagatgat aataacaagc cactttcaga taaggaaaaa gctgaagcta
    16621 agaaaaaggc tgaagaggct cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag
    16681 caaaaaaata ttcacaagat acttcagcaa gtgatggtgg taaattagga ttcttttcaa
    16741 ggggtcaaat ggttgctgaa tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat
    16801 ctgatttagt tgaaactcaa tatggatacc acataattaa agtgacagac agaataaatg
    16861 agcaaacatc atttgaagat gctaaagaaa ctataaaaga ccaactgttg aaaaataaat
    16921 atcaagaaca aatagaaaaa ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa
    16981 taaataaaat cacaatctaa gacttaaaaa aagagtaata tgcttaaaaa agctatatct
    17041 ggataagata tagctttttt tgtatatttt tcttaacaat ggtaaaaata aacttaaagt
    17101 aatttatgga gggattaaaa aatatgaaag caacaggtat agttagaaga atagatgatc
    17161 ttggaagggt agttatccca aaagagataa gaaagacatt aagaattaga gaaggagacc
    17221 ctttagagat attcacagca aaagatggag aagtcatact taaaaaatac tctccaatag
    17281 gtgaattaaa tgaattttcg caagaatatg ctgaaacatt agctgaagta ctaggatatg
    17341 gagtgttgat aacagactta gattctataa tagctgtgtc taagcttcct aaaaaagatt
    17401 ataaagaaaa aagtataagt gatgagttag aagcaataat aagtgaaaga acagtaaaat
    17461 attcaaaaga taaaaacatt ttaccattat tcaaggaaga ccaaaaagaa tatactagtc
    17521 aagttatcat gcctataatt agtgcatctg gtgactgtat aggttcaatt gtagttgttt
    17581 caaaggataa agatacaatt gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact
    17641 ttttaggaaa acaagttcaa taattttatc ataaagacct tttacatgaa ggtcttttga
    17701 agtttttgtg taaaatattg aatatagcat taaaaatatg gtatattaaa gtttgttata
    17761 taaacagtat ggaggttatg ttatgaacaa taacaaaaat aaagactcct ttctaaaagg
    17821 agcccttata cttgggctag caggtgtaat cgtaaaaata atgggtggat tttttaggat
    17881 tccccttgga aacatgattg gttcagaagg aatgggatac tatcaggctg cttatcctgt
    17941 gtatactcta tttttaacat tagctactgc tggatttcca acagctgtgg caaaattggt
    18001 ttcggaaaaa gttgcaattg gaaattttaa aggtgcaaat aagatattta aggtatcaca
    18061 tacagtttta ttcattaccg gtataatatc attttgtata ttattttttg gagccgacta
    18121 catagtaaca aacgtaatga aaaatccagg tgcgttatac tctatgaaag ccattgctcc
    18181 agcattacta tttgttcctg caatgtctgc atatagagga tattttcaag ggagacaaga
    18241 tatgactaag attgctgtgt ctcaagttgc agagcaattt tttagagttg tattaggtct
    18301 tactttggct tacttgctta tgaagagtct aggtcagact tttggtgcag caggtgctat
    18361 atcaggggct acaataggtt caatagcatc tatgctttat ttggtgtttg cttatatgct
    18421 agggagaaaa gagcgtagag cagagataga tgcaagccaa agatttaaag atgaaagagt
    18481 gtcttatatt tttaaaaagc ttttaactgt tgccattcct ataacaatag gagcatcagt
    18541 aatgccactt gtaaatatga tagataatgt tattgtaata agaagactta tggaagcagg
    18601 ctttacatac aaagtggcaa attccatgtt tggtcaatta acaggtatgg caatggccac
    18661 aataaattta ccagcagtca taacaactgc aatgagtatg agcttagttc cagcaatttc
    18721 taaagcatat gcattaggaa ataagtcaaa agctagaaaa gatactaaga gtgcagttaa
    18781 ggtaacttta cttatagtat tgccttgtgc gtttggtatg gcttctttag caataccaat
    18841 aatgggtctt ttatttccac atgaaccatc ttctgtagga actattttgt ttactcttac
    18901 tccatgtgta ttatttttag gattaataca gacattgact ggtataattc agggaatggg
    18961 aaaaccaata gtacctgtaa ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag
    19021 ttatacttta acaggtatac cagatattaa tgtactgggt tcagcatttg gtacagttac
    19081 agcatacttt gtggcagcca tgattaactt actatatgtt aaaaaacaca tgaatgtaaa
    19141 cttttctaaa aaagaattta taataaagcc atttataaca gttatgacaa tgtttataat
    19201 ggttaagttg agttatggtg ctttagttgg attcttagga aacagcatat ctacaataat
    19261 tgcaatatgt gttggtggaa ttgtatatgt agtagtaata cttggaatag gtggaattaa
    19321 aaaagaagag attctaacta tgccaaaagg tgataaatta tataaattgt taaaaaaagt
    19381 taaacttata agataatata ggaatatata tgcaaataaa ggaaatacta tacaaatagt
    19441 aaatctcctt atttgtataa taatataaaa ataaagtgaa atttgctaat aggagtgatg
    19501 ttatgggtaa gataagtatt gtaggtcttg gaccaggaga ctattctctt ataagccaag
    19561 gtgtgttaga atctttaata aatagcccta aagttttttt tagaacagag aaacatccaa
    19621 taattgataa acttaaagaa actatacaat acacttcttt agactacttt tatgaaaaag
    19681 aggaaaattt tgataatgtt tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa
    19741 agggtgacct agtgtatgct gttcctggtc atccaagagt tgcagagaag actgtaagta
    19801 taatagagag attagccaat gaaaagcaca tagcagtaga aatattgcct tctatgagct
    19861 ttgtagacgc tatgtttaac tatttggcta tagacccatc tgagggattt aaactggtag
    19921 atgcttttga aatagaaaat tcatatatag acttggatac aagtatgata ataactcagg
    19981 tatatgataa atttatagca tctaatgtaa agttaaaact tatggaacat tatgactata
    20041 atcaagaagt atgtatagta aatggagctg gtgtcaaaga tttagaaagt aaaaagttta
    20101 taaagctttg tgaattagac agaaatgaaa acttttttga ctatttaact agcttatata
    20161 tacccaaaag ttctaaaaag atgtataata cagtacatga cttagaaata atagttaata
    20221 cacttagaag tccatctgga tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa
    20281 attctgttat agaggaagca tatgaacttt gtaatgcaat tgataataat gatatagatg
    20341 aaatggtaga agagttaggt gatgttcttt tacaggttat attccattgt cagataggaa
    20401 atgaggaggg ctactttgat ttaaaagaag tagtaaatgg catatgtaaa aaattaattc
    20461 atagacatcc tcatatattt agtggtgcaa aattagatat gtctaagttt gagaaaactt
    20521 gggaagaatt aaagaaagaa gagaaaggcg aaagtactat aacagatggc cttagaagga
    20581 taccaaaaca tttaccagct cttatgaagg ctagtaaagt tcaacataaa gcagcacttg
    20641 tcggctttga ttgggacaat gtagaagatg tatttaaaaa aattgaagaa gaatatcagg
    20701 aattacttga cgaacacagg gtcgggaata taaaatacat aaaggaagag ctaggcgatt
    20761 tattattttc gatagtaaat ctagctagat ttttagacat agactcagga gaagccttga
    20821 actgtactac agaaaaattc ataaatagat ttgactttat agaaaagagt gcaatgaact
    20881 tgaataaaaa attagaagac atgacacttg aagagatgga tgagttttgg aatcaggcta
    20941 aaaaacaata aaattgtgga aggaaatttg tcaaaaatct agaactttta tcacaaagat
    21001 agaagtctat attataaaca tttttaggag gtaaatatcg tgaataaagc tgaattagta
    21061 tcaaagatgg cagaaaaaag tggattaaca aagaaggaag cagaagctgc gttaaacgca
    21121 tttatgagtt ctgttcaaga tgcactagta aataatgaaa aagttcaatt agttggattt
    21181 ggaacatttg agacaagaga aagagctgct agacaaggaa gaaatccaag agatccagag
    21241 caagttatag atataccagc ttctaaagca ccagttttca aagctggaaa aggattaaag
    21301 gatataataa atggataatt agaaatcttg tgaaaaagtg tggataaatt tgccacactt
    21361 tttctataga accaaggaag gtgataatta tgagattaga taaattttta aaagtatcta
    21421 ggattataaa aagaagaact gttgctaaag atgcatgtga taaagggatt gtcactataa
    21481 atggaaaagt tgctaaatct tcttcagagg tgaatattgg agatacatta gaaatacaat
    21541 ttggagagaa gaagatgaag tttaagataa atgaagtaaa agagcatgta ttaaaaaatg
    21601 atgcaaaaga aatgtacgaa attttagaat aaaattaata gtcctctagt atattaatag
    21661 tagtattatt gaaatattta ctggggggat attatggaac agaacataac tttgaaagat
    21721 agatcaaagc tggttatttc gggagtcgag cacatatact cttttaatga caaaagagta
    21781 gagctcaaaa cttctgtagg cgagatggta attgaaggcg aaaacctaga tatgagcaag
    21841 ctaagtatag acgaaaatat aattagcata gatggaacta taaattctat ggtatatgca
    21901 aagcctaaaa agccacaaga gagtttcttg aaaaaggtat ttagatagat gatacctttt
    21961 actcaagatg taagcatttt ttatgctact atttatggtg gaatactcat aggggtgttg
    22021 tttgattttt atagagggct aagagggaat tttaaattca tcaattattt tgctataata
    22081 tttgatgtat tgttttggtt tctagctacg gttataatat tcgtaacaat aaaccttaca
    22141 gaattttttg atttaagata ttatcatttt gtggctttgt ttattggttt tattttatac
    22201 tataacacca taagtaaaat tgttttatca attatcaata aaataattcg ttttgtgaga
    22261 aattctttta aaaaagttac acattacata gtcagttttt taaataattt gtattacgtt
    22321 ataatctatt cactacacct actatttgat attatatttt atataccaaa catatttata
    22381 gcaacaagaa aaagtataaa aagaaaatca aataaaaaat taaagaacaa gaaaaaatca
    22441 aaacctaaga agaaaaaaaa taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa
    22501 aaaaattttc aggacagatt attgttatca gtttattttt aggaatatca atttttagta
    22561 tgatgacagg atttgtattt gagtatacaa aagctaagga atataaaaaa gaaatagcaa
    22621 gtctaaataa acaattaaaa aagactgaaa tacaaataaa cagcttaaag aaagatgaaa
    22681 aatcatatga aggagactta gaggatatag caagaaaaag attgaatatg gtaaaaccaa
    22741 atgaaacagt ttatgtagac ataaatcgtt gattaaaact tgctaataag tttacagatg
    22801 tattgatttt aaaatttaag atttaataaa aaagtcattc agttaaggat ggctttttgt
    22861 ttttattgag gagggaattt cataatgaaa gtaggagcta tagatattgg gacaaactca
    22921 atgagattat tgatagcaga ttatagagat gggaatttta taaacagaaa aaaatttgtt
    22981 aatacaacta gaattggtta tggtgtggat agtaaaggat atataaaaga agatgctatc
    23041 aaaagaaata taaaagcatt aaaagaattt tctgataagt gtaatgatga aaagtgtgaa
    23101 aaaatttatt gtatggggac atctgctctt agagacagta aaaatggaga ttactttgtt
    23161 aaattagcta aagaagaaac tggaatagat gttgatatag ttacgggaat agaagaatca
    23221 aatttgggct ttaagggtgt tttagaagga atacaaggtg atgatactat tattgttata
    23281 gatattggag gaggttcaac agaatttatt gtaggtgatt cagaaggaat tatatttaat
    23341 aaaagtgaga atgtaggtgc tcttaggatg acagagaggt ttttgaagca cgatattatt
    23401 gaagatgaag agtttgaagc tatgagttca tttatatatg gtgagattga taaaacgtta
    23461 aatcatataa aagacaaagg attaaaaact cttataggta taggaggtac gattacatca
    23521 ttatcagcaa tgaatcaaga attagagatt tactctatgg aaaaaataca taatagtaaa
    23581 atacacaaaa aagaaataga agaaattcta caaaaattaa taaagatgac attaagtgat
    23641 aaaaagaact taaaagggct acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa
    23701 atattgaata taataatgga aaaattagaa atagaagaaa taaccgtaag tgaatacgat
    23761 aatttggaag gcttaatatg tcaaaagtca aaaaaaatgt attaatattt tttgattttt
    23821 aaaaccgatt atgacaaaaa aaattactcc tctttgttac aattcttaat ataaacaaaa
    23881 gaggaggttt ttttatgcaa agaagtgtag cagttgtgaa gaataagact gtaaaaccaa
    23941 atgtcataaa attaataagc ggatatattg attttactac ggcggcattc gctgtgatgg
    24001 gatttttact tagtaggtct attatagtag actctgtagc tcctttagga attgcctttt
    24061 ttatatgtgc atcaaagatt gataagtaca agatacctgt attcatatca acattattag
    24121 gaatattgtt atcatctaat gattttggaa atgtagcaaa atatgctgtg tgccttacaa
    24181 tatttatggc tatgaataaa aaattaaaat tatcagattc aattgctaag gttgcattta
    24241 taggtgcaat aatactactt ccaatctcca tgggacaagc tttcttttca aataggtatg
    24301 tatatggtct tcttatagca ggagtagaag caataataat gtttatatct atatatgtgt
    24361 tttcttatgg gataggttta atggccaatg tcaacaatag gatgtctgta cggacagaag
    24421 aagccatatc aataagttta ctgctaacct ttagcataat gggaatagga gatatatctc
    24481 tgtttggaat atcaataagg accattttat cgaccgtatt gatattgctg acctcaatag
    24541 taggtggagc tactatggga gcatctagtg gtgtaattat tggtgtagca tccattttaa
    24601 ataatatgac atcagctgtt tatatgggga tatattcttt ttcaggatta ataagtggag
    24661 catttaataa aataaataaa tatttctgta tactaggata tattctaagt tggacaataa
    24721 tatacctata tacttctggt attacttcta atatgatgca attaagagat atactattag
    24781 gttgtttgat agtactagta ttgccagagc gattatttaa taagattgaa aaattaataa
    24841 aaagtaatgt ggcttcaaat gaaatcgtat acgattatat tatgagaagc aaaaacctaa
    24901 ctaatagcag attgaacagt atttataaaa cttatgatga ccttgcagat acatttgata
    24961 agataagaga aaaagacaaa gtgctagacc aaagagacat agctaatgta atagatatga
    25021 ttcataatga tgaatgtaaa agttgtagta tgagaagaat gtgctgggag tcaagattta
    25081 atcatacata cactatgata tataacattc ttgaaaaaat tgaggagaaa ggggagttaa
    25141 gtctaaacga tatacctaaa aattttagaa aggaatgtat gaaaccagag tcaatagtga
    25201 aaatatcaaa tcactattac aaaatgtttg tattagatta tgattggagt gtgaagtttt
    25261 cagaaagtag aaagttaata gcaaatcaga taagaagtat ttcaaaatcc attaaatcct
    25321 tatctcaaga tttagaaggc gacataatgc tagatataga aaaggaaaaa aatatatatg
    25381 aacagttaga aagatatgat ataacagttg ataaagtaag ttacttaaca aaaagtaaca
    25441 gtgagtttga aatatcaata gaaaagaaaa cttgccatga tggatgtatg tgtgaagata
    25501 aaattgtaaa tataatttca gacctagtag gggaaaatat gtctgttaga aaaataggat
    25561 gtcactgttt aggaggtaaa tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg
    25621 ttacagaagt ttcagcaatg tcaagggatg ggcatatcct ttgtggagac aattacacat
    25681 acatggagat aaatgatggt aaatacatga tggctataag tgatggtatg ggaaaaggta
    25741 aaaaagcata tgaagaatct tctgccacta ttgatatact ggaaaaaatg atagatgcaa
    25801 aaattaaaga tgagatagtc attgatacta taaataatat gttgttgtta aagtcttcag
    25861 aagagatgtt ttcaactctg gatttgggaa ttttagattt aaaacgaggt tgtttagaaa
    25921 ctataaagat gggagcgtgt tctacttata taaaaagaga ggatggagaa gttgacttaa
    25981 tatcgtcatc atcattacct gtgggtatat tatcagatgt aaaaattgat agaaaaaatg
    26041 taaaagttaa ggaaggagac tatgttataa tggtttctga tggaattgta gatgcaggaa
    26101 gaaataacaa tttaggagac aattggttaa tatacttctt aaagaatata gagactacaa
    26161 atccaaagga aatttcaaat ttaatattgg atagagcatt agagttacaa gcgttacaaa
    26221 ttgaagatga catgactgtt ttagttacga aaatatgtac aaaataaggt ttacaagatt
    26281 atgtgtataa gatgtggata acttgtgtat aatatgttaa tagacttttg aaaaaagtat
    26341 agttattaac aaaaataaca tataaatcaa caaaatttgt tgataagtat ggcagaaatg
    26401 ttaataaaat gtaaaaaaag aaaaaacaaa agctattatc aaaagagatt catgttgaaa
    26461 taactaaatt tctaagtaaa gaaaacctgt gaatatgttg attattatgt ggataactat
    26521 taattcgata tttatttata tagtatgaat aattgaataa tatatctaaa aaaagccatc
    26581 ctattattaa gtaaacttat tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc
    26641 aataatacct ttatttattt ctattttttg catgttaata ataagtataa aagctgacca
    26701 aggatatcaa aagaataatg atagagcaat tactgtcatg aataataatg atactgaaga
    26761 tgatgctgaa aaaaaacaag aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt
    26821 gtttaaaagt gatgaccaat ggaaaaaaga acttaagagt tttgataaag atactaaaga
    26881 gttgaaaaat tacataggta aagtaacaaa atcgccaaca catttgtctt ttgccttaaa
    26941 cattaaagaa aaacttgata taagactaaa taaactttct gcctatgtaa aattaaaaca
    27001 agatactaat aaaaattcgt ataagtactt agatatgaat gatagtatga gtaaatccta
    27061 tgggaattat atggggattt gttcagattt agagttagaa atactaaaat tatcaaacaa
    27121 agattacaag aaaattattt caaataaaaa tataaatagg aaatatggtg catatttaag
    27181 agatataaga aggaataaag tacattattt ggatgataaa tcagaagata ttctaagcaa
    27241 agtatcttca ataagtagtc ttccaagcca agtatatgaa ttatttagaa atatggatag
    27301 aaaaactaat ttgacaccag ctcaatatgc gagtgaatta gagtcatcag atagagaaaa
    27361 tagaaaaaaa gcttatcaag atgaacttat aacttacaat gataatataa atacaattgc
    27421 aggtttaatt actggtcaag ttaaaaagaa tattttttat tctcaggaaa gaggttatga
    27481 gtcttcttta gaaatgtacc tggaatctga taatgtagat gaaaaagtat acaataattt
    27541 gataaggact gtaaatagta atatggacag tctacacaaa tatatagatt taagaaaaaa
    27601 agtattaaaa ttagataagg ttcattcata tgatatgttt gtgcctatgg ttaaggctgt
    27661 tgataataat attaattatg aaaaggcaca aagtatagta tattcagcgt tgtcaccttt
    27721 aggaaaagaa tacaatgatg tagtatataa agctttcaat gaaaaatgga tagatgttta
    27781 ttcacatgaa aataaagtaa gtggtggata ctgcttatct gtttatgaaa atcatccata
    27841 tatactttta aattataata attcattagg ttcagtttcg actttatcac atgagcttgg
    27901 acatgctgta tatgaatatt taagttctaa gaatcagaat tattttaatt caaatccgtc
    27961 tatatttact cacgaagttg catctacaac gaatgaagca ttactttacg agatgcttat
    28021 aaaagaagct aaaggcaatg atgaaaaagc ttattatata acacagtatt tagacttgat
    28081 aaaagatact ctttatacac aaactatgta tgctgaattt gaaaaaacta tacacgagtt
    28141 agtagaaaaa ggaaaaagtg taaacgcatt agtattaaat gatgtttggg gacaacttct
    28201 taaaaaatat tatggacaaa gctatgaatt agaccaatta tctaaggtcg gatgggctag
    28261 aatcccacat ttttataata gtttctatgt ctataaatat gctacaggtt gtagtgcagg
    28321 agtaagtttt gcccaagaca tcctaaagaa tggttcagat aactatatga gctttcttaa
    28381 aaagggaagt tcagattatc caataaactt gttaaaatca agtggcataa atttaactaa
    28441 tacaaaaccc atagaaaata caatcaaaaa atttgatagt ttagtagcag aacttgaaaa
    28501 gctattataa acattattgt aattcacatg gtaaatatat cgaattaatg ataaaatata
    28561 ctaaaagttt aaagtcctat caagatagga ctttttattt attaattagt gggagatgat
    28621 tctatgcaag ttaaagttaa aaaggctgta ataccagcag caggtttagg aactagattt
    28681 ttgccagcta caaaggcaca accaaaagag atgttaccaa tagttgataa gccaactcta
    28741 caatacataa tagaagaagc tgtggcctca ggaatagagg aaatactaat aataacaggt
    28801 agaaataaaa aatctattga agaccacttt gataaatctg tagaattgga acttgattta
    28861 gagaaaaaag gtaagaaaga attgttggag gtagtacaaa acatatctaa catgattaac
    28921 atacattata taagacaaaa agagccaaaa gggttgggag atgcaattta ttgtgcgagg
    28981 cattttatag gtgatgagcc gtttgcagta atgttagggg atgatattgt agataatgat
    29041 gtaccttgtc taaaacaact aacagatgca tatgaggaat atagaactac aatattgggt
    29101 gttcaaaagg taaatcaaga agatactaat aaatatggga taatagaagc aaaaaatata
    29161 gaaggtagag tttacaaggt aaaagacatg gtagaaaagc cagagtcagg aaaagctcct
    29221 tcaaatattg ctattttagg tagatatata ataactcctg aaatctttga tattcttaag
    29281 gatttaccac caggtaaagg tggagaggta caacttacag atgctttaaa aatattatct
    29341 aagaaagaag ctatgtatgc atacaatttt gaaggcaaga gatatgatgt tggggacaag
    29401 ctaggatttt tagaagctac tgttgacttt gctctaaaga aagaagacct taaggaagat
    29461 tttataaaat atttgaaaca tgtatgtagt gaatttgata aaaataacaa tgtgttatac
    29521 aataatacag atgaaaaaat agatttaatt gaagtcaatg aagctagaga acttgaaatt
    29581 caaaaataat taatgaaagg tgatgtatgg tggcaaaatc aagtcagaaa agaattaata
    29641 gaataatagc tgttgggatt gtgggaatcc tcgtaataac gagtataaca gctgttttga
    29701 gtatgattgc aactatgtaa taagataaat gacagcagtt gatatacata aatataaaaa
    29761 ccatatataa cttgatgtag tcaaaaactt acaatatttt agttatatat ggtttttaga
    29821 atttataaat gtaaaaaaaa atttttataa tagttgcata ttttaaagga aaatattatt
    29881 ataagatata aatatagtga taaatttata aaaacgtgct aagattggac ttgaaataca
    29941 gtaatatagt aggtaataat gttttaaatt ttatttattg accataatat ataaataatg
    30001 atattattag acttaactgt ttaaataaaa cttttctttt tcatgccttc aatttccaaa
    30061 ataagtatgt atataaatga atatatagat gaggaggatt tttttgattt tagatgataa
    30121 agtattagaa ggattaggta gtaagacctt agttgagcta cgagagatag ctaaagaatt
    30181 aaaaataaaa tcaattacta catataagaa aaatgagtta attgagatta taaattccaa
    30241 aagtaaaaat gaggaaaaag taaacgaaga gactaaagaa aaaaatgaag atattaaaga
    30301 agacataaag aatagaaaag aaaatataga gcgtgaaagt atagaaaaag aagttgaata
    30361 taagagccct accaaatcat acaacaagaa agaaatagat acccaaaata tttcccaaaa
    30421 ccaagaacat agaaatcaaa gaaatgacta taataagaca acgagtgtaa atgatagtaa
    30481 taagtcatca aatatatcta atagaatggt cagaaataat aataaaaact attatatgcc
    30541 taaacaagtt gatgaatcta agatagtaga tgaatttaac acatcaaaag aggatgaagt
    30601 agtaggtgta cttgaaattc tgccagatgg atttggtttt ttaagaggtt caaattattt
    30661 gtctactgaa ggagatgttt acgtttcacc atctcaaata agaagattta atatgaaaac
    30721 aggagacaag attaagggaa taactagaca tccaaaaagt ggagagaaat ttagagcact
    30781 tttatatgtt caaaaaataa atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt
    30841 tgagacatta acaccaatat ttccagaaga acgacttaca ctggaaacta atagaaatga
    30901 aatagctaca agaattatag atttaatttc acctatagga aaaggacaaa gaggattgat
    30961 agtagcacct ccaaaagctg ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc
    31021 taaaaatcat ccaaatgttg aacttatagt actccttata gatgaaagac cagaggaagt
    31081 tactgatatg aaagaatcta ttgaaggaga cgttatatat tccacttttg accaagtatc
    31141 aagtcatcat gtcaaggttg cagagatggt gttaaataga gctcaaagat tagttgaaca
    31201 tggtaaagat gtagtaattt tactagatag tataaccaga ttggcaaggg catataacct
    31261 tacaatatct cctacaggta gaactttatc tggaggtatt gaccctggtg cacttcatgg
    31321 acctaaaaag ttctttggtg cagcaaggaa tattagacag ggtggttctt tgacaatatt
    31381 aggaacagca ttggttgaaa ctggttctag gatggatgat gtaatatttg aggaatttaa
    31441 aggtacagga aatatggagc tacatttaga tagaaaatta gcagaaaaaa gaatattccc
    31501 tgcaatagat atttacaagt ctggtacaag aagagatgat ttattgcttg atgatgaaga
    31561 aaagactgct ctttggagat taagaagaga aatgagtaat aattcagtaa tggaaattac
    31621 agataaagtc attgaactta taaagagaac taaggacaat aaagagtttg taaaatcaat
    31681 aaagaatttg taaaatcaat aatacaaatg caaatatgga ttatataaac taatagaaaa
    31741 atcacttgaa tggcataata agttgtgcta taataatgta gttgataatg taaaaagaaa
    31801 gttaatataa ttaatttttt taaataaaga gaagaggtga ctataatgca aaaggaaata
    31861 caaccaaaat acaatccagt tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt
    31921 tcaactaagg acgaaataaa agttgaaata tgttcagagt gccatccatt ctatacagga
    31981 aaacaaaaga acatagaaaa aggtggaaga atcgacaaat tcaagaaaag attcaaaatg
    32041 gactaatttt tataaaagtg gagttggagg aagccaactc ctattttatt acaaaaaaac
    32101 attcatattc tactttaaat tgtctttttg tattataata ataaatatgt aaaattgagt
    32161 attctaaata caataaaatg aggtgagtgg atggcaaaac aagctgtagg tgggcaagct
    32221 gttattgaag gtgtaatgat gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt
    32281 gacggagaaa tagcactaaa agaagataga ataaaaagtt gggtaagaga taagaatata
    32341 gataagatac cttttgttag aggttctttt gttatgatag atacaatgat tcaaggcata
    32401 aaaagtttaa acttttcttc agagtttttt atggaagaag ctgaagagga taaatttgat
    32461 ttatttataa agaaaatttt taaagataag gcaaatgata taattataat attttcattg
    32521 gtcatagcaa tgttactttc tgctggatta ttcattttta tacctacatt ggtaggagga
    32581 gctttttcta aagtaatgcc taatgatttt atgcttaatt taatagaagg aatcataagg
    32641 atagctatat tatttgcata tatagtattg atttcacgaa gtaaggatat agaaagagtc
    32701 tttcaatatc atggagcaga acataaatct atatattgct atgaaaatga tttagaactt
    32761 acagtagaga atgctagaaa atttaaaaga ttacatccta gatgtggtac aaatttccta
    32821 tttatagtta tggctgtatc aataatattg tttgcatttt ttggttggcc aaatccaatt
    32881 cttagaatat ttatgagaat tatatgtgtg cctatagtag caggtttatc ttatgaggta
    32941 attagagttc ttgggaaata tgataatgga tttactaaaa ttattgcata tccaggaatg
    33001 atgttgcaat attttacaac aaaagagcct gatgatgagc aattagaggt tgctttggaa
    33061 gcattgaaag cagtagtaga ttagagaaaa ggatatttgt tatgacgata aaagatataa
    33121 ttattaaata ttcagataaa ctaaaagata taagtgatac tccaagatta gatacagagt
    33181 tattgttaca aaaaacattg ggtgttgata gattatatat tcacttaaat ctgaataaag
    33241 agctaactga agaacaaaaa acaaaattta tgggttttgc agaagaaaga ttaaatggaa
    33301 gaccaatagc atatatagtt gaaaataggg agtttatggg attagatttt tttgttaagg
    33361 aaggcgtatt gattccaaga ccagatacag aaactttagt agaagaaata attgaaatat
    33421 gtagagaaaa aaaagatgta agtatattag atataggaac tggttctgga gctattacaa
    33481 taagtttagc aaagtacatt gaaaattcta aaatcatgtc ttttgatata tcagagacag
    33541 cattagaaat agctaaaaaa aatgctatta taaatgaagt tggtgaaaaa ataaagtata
    33601 ttaattctga tttatttaca gcaataagtg atagtaacat aaaattcgat atcatagttt
    33661 caaatccacc atatattaaa aaacaagata tagaaactct gcacaaacaa gttaaagatt
    33721 atgaaccata taatgctctt gaaggtggag aagatggatt agacttttat agaagaataa
    33781 cagaacaagg taagaaatat ttaaataagt gtggaatatt agcatatgaa gttggtcata
    33841 atcaagctga agatgtaata aatattatga aaagtaatgg atataaaaaa atatacacaa
    33901 agaaggatat tcaaggcatt gatagagttg ttataggtta caatatatga tataatcaac
    33961 aattgatatg ttacatttaa tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa
    34021 gatacatata aagacttaag tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa
    34081 gtttggcaga aatatataaa agaacatgct gacttggaac ctattgttat gaaatacaga
    34141 gagtataaaa gcgttttaga tagtataaaa gaatcaaaag aaattttaca agaagagtca
    34201 gatgaagaat taagagaatt agctaaaatg gagttagctg agatggaaga aaaagtagca
    34261 cctctggagg aagaaataaa aatactatta ttgcctaaag accctaatga tgataagaac
    34321 gttatagttg aaattagagg tggagctgga ggagatgaag cagctctatt tgctggtgat
    34381 ttatttagaa tgtactcaag gtatgctgaa agaagaagat ggaaaataga attattgagt
    34441 gcaagtgata ctggagttgg tggatataaa gaagtatctt ttatgataaa aggtaaaggt
    34501 gcttattcaa gattaaagta tgaatctgga gttcatagag ttcaaagaat accatcaact
    34561 gaatctggtg gaagaataca cacatcaact tctactgttg cagtattacc agaagttgaa
    34621 gatgtagagg ttgaaataaa tccaaatgat ttaagaatag atgttttccg ttcttcaggt
    34681 aatggaggac aaagtgtcaa tactactgac tctgctgtta gggtaacaca tatacctaca
    34741 ggtgaagtag tatcttgtca agatggaaaa tcacaattaa aaaataaaga gcaagcttta
    34801 aaaatattaa aagctagact ttacgataaa gcattggcag aacaacacaa agatatagca
    34861 gcagaaagaa aaagccaagt cggaacagga gacaggtcag aaagaataag aacatataat
    34921 ttccctcaag gtagaataag tgaccataga ataaatttaa ctttatataa gttagatgca
    34981 ttcttagatg gagatataga tgaaatgata gatgctttaa ttactgttga ccaaacagaa
    35041 aaaatgactg caatataagt aatgttttaa agaagctgtt tcttcctaga aattattttc
    35101 tattttgaaa cagctatttt gcattttagg tcatgttatc atattctatt gtatataatt
    35161 gttatataca agtataggag ggttatatga tactaaaagt aacaataatt gggcttttgg
    35221 caggtgttat agggacagga ttaggaggag taatatcagc catttttaaa agagaggtcg
    35281 ataaatatct aagttttttt atgggattat ctggtggaat aatgttggct gtagtggtgt
    35341 ttgatttaat gaaagaatcc atggacaaaa tggggataat caatacagtt atatttacgt
    35401 ttgttggagc tctcataact atgtacataa aaacaaaatt agatgtgtct ggaaatatgg
    35461 catctggata tcttatattc ataagtatat tactacataa tttaccagaa ggactagcca
    35521 ttggttcttc ttttatgtca actgaaagtt taggtattac acttgctata gttataggtc
    35581 tacataatat accagaaggg ttagctatgg cattaggctt agtttgcaat aaaatgaaac
    35641 taagtaaagt tattttgttt actgtaatag ctggattacc tatgggtcta ggaagttttt
    35701 taggtgttta ttttggagga gtatttactt ctcttatagg agtatttctt gctacagcag
    35761 gaggtactat gatgtatgtt gtattggaag aaatttttcc acattcaaaa agtgtgtatt
    35821 gtataattgg tttcttgtta ggtacaatga ttgtaaatta tatataaaat taaaatgaaa
    35881 gcaggcatac aaaagatgaa aactattata agtaatattg atataaataa tattgattat
    35941 gaggaaataa aaatacaagc aaaacttctt agagaaggta aaacggttat ttttccaact
    36001 gagacagtat atgggttggg tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat
    36061 gaagctaaag gaagacctag tgataatcca ttgatagtcc atatatatga aaaagaagaa
    36121 gtatatgact tagccaaaga tataagtgat aaagctaaac ttgttataga aaagctttgg
    36181 ccaggaccta taacaattat attaaataaa aaagatataa ttccatacaa aactagtgga
    36241 gggcttgata cagttgcaat aaggatgcct tctaatgtta ttgccagggc aataataaaa
    36301 gaagctggta tacctattgc agctccttct gcaaatatct caggtcgccc atctccaaca
    36361 aaagcaaaac atgtatatga agagatgaat ggaagagtgg atggaatagt tttaggagga
    36421 gacagtaatt ttggattgga gtcaactgta ttggatttaa cagaagagac tcctatgata
    36481 ctgagaccag gaagtataac taaagaagtt ttagaggaac ttcttggaga agtaaagctt
    36541 gacccatcat taagtaaaaa agaagataat caaaaagcta aagctcctgg aatgaaatat
    36601 aaacattatt ctccaaatgc agatgtatat ataatatctg gaaaaagaga aaatgtagca
    36661 acaaagatta atgatatgat aaaatctaat agagacaaag gtctaaagac aggtgtaatg
    36721 tgtattagta gaaaccgaga gttttatgat ggagaagtaa tcgatttagg aaactctctt
    36781 gaagaagttg caagtaatct gtttgatgct cttatagaaa tggacaaaaa gggtgttgat
    36841 gtcatatatt cagaagagtt ccccaagact ggagttggac aagcaattat gaatagacta
    36901 ctgaagtcag caggatacaa agtgataaag gcttaagagg gagttgtaaa atatgaatat
    36961 acttattgtg tgtacaggta atacttgtag aagccctatg gctgaggcta tactaaggaa
    37021 agcaattaaa gaatctggaa gaagtattga agaatattct atatcatcag caggtatatc
    37081 tacagcaaat ggaatgggtg cttctgaaaa ttctatagaa gtactaaaag aaatcggtat
    37141 agacttatca aatcatagaa gtaaagtaat tactaaaaaa ttaattgatg aatcagatat
    37201 aatactaaca atgactaaat ctcataaaga aattttagta caagcagttc caaaatgcaa
    37261 agaaaaagtg tatactttta aagggtttgc aaataaaaat gaggaagata tatctgaccc
    37321 atttggtgga aatttggata tttatagaag cactatgaga gaaataatgt attctgtcaa
    37381 cgaaatagta aaaaaaatat gaaacggagg ataaacaaat gaagatagga ttaggctgtg
    37441 accatggagg atataattta aaaaaagaaa taataagcta tttagaaagt aaaggtatag
    37501 agtgtgtaga ttatggaact aataatgcta cagattctgt tgattatcct gtttatggag
    37561 aaatagttgc aaattcagtt ataaataaag aagtagatta tggaatatta tgttgtggta
    37621 cagggatagg aatatcttta gctgctaata aggttcctgg gataagatgt gcagttgtat
    37681 cagatgtatt ttctgcaaaa atgtcaaaag cacataatga tgcaaatatg ttatcattag
    37741 gcgaaagagt tttaggaaaa ggtctagcac ttgagatagt tgaagcatgg attaatacgg
    37801 attttgaagg tgatagacat gctagaagag taaacatgat taaatcaata gaagaaaaac
    37861 ataataaata ataaaaatta cagttttatc aggaggaatt atcaaaatga gcaaagtggt
    37921 agagacaaac catccattaa tacaacataa attaacttta atgagagata aaaatactgg
    37981 ttctaaagac tttagagagc ttttaactga aattgcaatg ttaatggggt atgaaataac
    38041 aaaagatata ccattaaaag atgtagaaat agaaactcct attcaaaaaa catcatctaa
    38101 agtagttgca ggtaaaaaat tagcaataat tccaatttta agagcaggtc ttggaatggt
    38161 agatggatta gtgagcttaa tgccagcagc aaaagttgga catgtaggac tatatagaga
    38221 cccagaaaca ttaaaaccag ttgaatatta ttgtaagctt cctcaagaca taggagaaag
    38281 agatataata gtagttgacc ctatgcttgc aacagggggt tctgctgtag cagctataga
    38341 tttacttaag tctaaaggag ctaaaagtat aaaattagct aatttagtag ctgcaccaga
    38401 aggtatagca gaagtgcaaa aatatcatga tgatgttgat atatatgttg catcagttga
    38461 cgaaagatta aatgaacatg gatatataat tcctggtctt ggtgatgctg gagatagatt
    38521 gtttggaact aaataaaaat aaataactat aaatatttat tagggacttt cttatatagt
    38581 gtatataatt ttatgtaaga aggttctttt ttatatattt tttctcaaaa taggaattat
    38641 tgaattgata aatttctcga attgagaata aaaagttttt accaattaaa aaagattgct
    38701 tattttacaa ggttttatag aatagtagtc tattttaatg tggcatgtta gttgcttacg
    38761 attataggta ttaagtgtat tagttaaatt gagaattgaa tattaataat attattggga
    38821 gtggtaatac atgaagaatt gtatagtagt aagaggggct ggagatttag caacagcagt
    38881 tattcataaa ttacacaaca gtggttttaa agtagttgcg ttagaaatag aagagccatt
    38941 agctataaga aggcaaattt ctttttgtga ggctgtttat gaaaaaaaag taactgttga
    39001 aggtgtagaa tgtatatttt gcaatactga aaaagaaata gaggaagttt tatctcaaca
    39061 taaagtagcc ttgattatag acccaaaagg agaaaaatta tctaaattaa atccaatcgt
    39121 tttggttgat ggaatactcg ctaagaaaaa cttaggtaca actaaagata tggcaccact
    39181 tacaatagct ttaggtcctg gattttgtgc aggagaagat gtagatgttg ttatagagac
    39241 aatgagaggt cataatctag gaagaataat aactaatggt agagcagaaa aaaatacagg
    39301 agtaccagga gcaataaaag gtttttctaa agagagagtt atgcattcac agtatgatgg
    39361 cataatggaa aatgtatgta agataggtga tatagttgaa aagggagacc tactttgcta
    39421 tattaataat tatgaaaaaa aacatgaagt tagagcaaca atatctggag tccttagagg
    39481 tttattaaag gatggagcaa aggtaaacaa aaatttaaaa atactagaca tagaccctag
    39541 aaaagaagaa gttaagaatg cttttacaat atcagataaa gctagatgta tagctggagg
    39601 ggttttagaa gccatactaa acaatgggat ttatccccaa aattaattta tattagataa
    39661 agctattaat attttattaa gatgatataa taaatttaat agattaaaga gagcatatga
    39721 cgggtattta aaatttaaat ggtaaaaatg ctcttttttt attggacatt caattaacat
    39781 gtgtatataa tataaaaata agtatttatt taggttgaaa tctaataaat atataatacc
    39841 agtacataag caaagaggag aaaagtaaat tatgagacca tcatgggatg aatacttcat
    39901 ggaaattgca gaagtagtga aaaagcgttc aacttgtatt agaagacaag taggagctgt
    39961 aattgtaaga gacaaacaaa tattaacaac tggatataat ggttctccga gaaatcttga
    40021 acactgtgaa aatattggat gtaagaggca ggaattaaat attccctcag gagaaaggca
    40081 tgaattatgt agagcattac atgctgaaca aaatgcgata atacaagctg ctcataatgg
    40141 tattagtgta gatggggcaa ctttgtatgt tactacaaga ccttgtgttt tgtgtgcaaa
    40201 aatgtgcata aatgcaggta tattgaaaat tgtttatgaa ggtgattatc cagatgatat
    40261 gtctacagaa ttattaaaag aagcaggaat agaactcata aaattttagt ggataaaatt
    40321 tatatgacaa taaatgtcaa attatgacac tgaaatacaa tggtatcaaa tgattaaaag
    40381 aaagtatttg aaaaatcatc agattagtag taagatatat tttataatta aataaattta
    40441 tgagatagat taattacatg ttatttattt tataataatg agggatacta tttatgagta
    40501 aaagaaatac acatacagaa attgcaaagt tgtttagttt aataagtcaa ataggattga
    40561 tgattgtaat atcaatatta ggatgtactt tcttaggaaa atttttagat agtaaattga
    40621 atactactcc agttctgaca ataatatttt tattgttggg tgttggaggt gcttttagtg
    40681 gtgtttataa aactctaata gtatatacac gaaggaagtg atatattgga tataaaatta
    40741 cttaaccagg taaggtcagt aacaaaaggt atagtaatat ttgatgtagt tttaattgtg
    40801 ctaatgttga taacatcaac attaagtaaa ccagtattat taggacttat atttggctct
    40861 ataatagcta tgttaaattt tagattattg gcgatatcac tggaaaaatc agtgacattt
    40921 cctgctggga aggcacaagc ttatgcatca gcacaatatg ctgttagatt atttataatg
    40981 gcagttgttc taattgcatc agctacagta ccacatttaa atataatcgg aacagcattg
    41041 ggattattga gcacaaagtt tgtcatatta tcaaaaaact ttataaataa attaaagagg
    41101 aaggaggcat aagtatgaat caagcgcatt attatctcat aggaaacttt aagattagtg
    41161 aaacaatact agtaagttgg ggaataatgg cctttttagc gatagcatct tattttttga
    41221 ctagaaactt aaaaaaagtt ccaactagta aagttcaaat attcttagaa tttgcagtag
    41281 gtggtcttgc taaaatggtt acagatgtta tgggtaaaaa tacagtaaaa agaatgccat
    41341 atatgatacc atacatagga agcttgtttt tatttttcgc atgttcaaat ttaagcccat
    41401 tacttggatt aaggtcacca acaaccgact tagacacaac tgctgcatgg gcaatgatta
    41461 cattttttat gatttatttt gcaggagtta aatttaaggg gttatcatac tttaaagaat
    41521 tggctcaacc acttgccata atgttaccaa tcaatattat aggtgagtta gctagaccaa
    41581 tatcattaag tttccgtcca tttgggaata ttttaggtgg aacaataata atgggtctgt
    41641 tttatcaact tttaggtttt atatctagtt taataccagg attatcaata ccaattggag
    41701 aaatattaat accagtccca ttacacttgt actttgactt atttgcagga ttgttacaag
    41761 catatatatt tataatgtta acaatggtat ttatatcaag tgcaggagag ttagattaag
    41821 aaatcaactt atattaagat aaaaataaaa taatttaaaa aattaaagtt ttaaggagga
    41881 aataattatg gaaacagcaa tagtagcggc agcatcagca ataggagcag ggatagcagt
    41941 tgcaacaggt ataggtgcag gtataggtca aggtatagca gcagctaaag ctgcagaagc
    42001 agtaggaaac caaccagaag ctaaaggtga tataacatca actttattac taggagttgc
    42061 tatagcagag tcttcagcaa tatatggttt ggttatatca ataatacttc tattcgtaaa
    42121 tccattcttt aaatacttag gaatgtagat ttagtcctta aatattttga gataggttta
    42181 taggtaaatc atctcaaact tataatcatg agatgaactt tatcatctcg tgattataag
    42241 tagttatgat ttatggaaag gaggataatt atggaaaaag cactagtagg tataacgtgg
    42301 gaatttgtgt tccaaattgt taatactttt ataatatttt tacttctgag aaagctttta
    42361 ttcaagccag tattaaatat aattgaatca agagaaaatg atattaaaag tgatttggca
    42421 gaaggcgaaa aagcaaaaaa tgaaggttta gctctgaaaa aagaatatga gtcaaaaatc
    42481 aattttgcca aggatgaagg tcaagaaatc ataaaacaag caactataag agcagagcag
    42541 aaatcagatg atatagtaaa tactgcaaag aaagatgctc ttgatataaa agaaaaagca
    42601 aacaaagata tagaacaaga aagacaaaag gttataaatg aaatcaaaaa tgatatatct
    42661 aacatagcac tacttgcagc atctaaagtc attgaaaaag atttagataa atctaaacat
    42721 gaagaattga tagaaaactt tataaaagag gtaggggaag ctaaatgata aatgtaatag
    42781 caaatagata tgcagaagcc ctatttcaat taggagaaga agaaaattca actgatgtct
    42841 tattcaaaga actagaaaaa gtagtagata tgatgactaa agttagtaaa gatttctaca
    42901 aagttttaaa atcaccatta gtttcaaaat cagaaaagaa aaacttggta gaaattattt
    42961 ttagtaaaga agtaagttct aacataaaaa actttttaaa agttttagta gataaagata
    43021 gaatatcata tttagaagat atcgaattag cttataaaga attattaaat aagaaaaaca
    43081 atgtaattga tggagtagca ataagtgcta ttccaatgag tgaaacagat attaaagaac
    43141 ttgaagtcaa actttcaaat aaatacaaca aaaatgttac tatagaaaat gttgttgaca
    43201 agactatact agggggagtt ctagttagaa ttggaaatga acaaatagat ggcactgtga
    43261 aaactcgtct tgataagatg aaagaaaagc tatctgaagt aatatcttaa gaatggcggt
    43321 gaacccatga acttaaaacc tgaagaaata agttctataa ttaaacagca aataaaaaat
    43381 tatgagaata aagttgagtt gacagataca ggtagtgttt taacagttgg ggatggtata
    43441 gcgagtgtat atggattaga aaaagctatg tctggtgagt tgctagagtt cccaggtgaa
    43501 atatatggta tggcacttaa cttggaagaa gaagtagttg gagctgttat ccttggagat
    43561 gacagtgaaa taaaagaagg cgatatagtt aagagaactg gaagaatagt tgaagttcca
    43621 gttggagaag ctttaatagg tagagttgta aactcactag gtcagccaat agatggaaaa
    43681 ggacctatag cgtacacaaa aactagacca gttgaatctg aggcacctgg tataatagat
    43741 agaaggtctg tttatgaacc attacaaaca ggaatcaaat caatagattc aatgatacca
    43801 attggtagag gtcaaagaga gttaataata ggtgacagac aaactggtaa aacatctata
    43861 gttattgaca ctatactaaa tcaaaaagga aaagatgtta tctgtatata tgtagcaatt
    43921 ggacaaaaac gttctactat agctcaatta gttagctctt tagaaaaagg tggagctttg
    43981 gattatacaa tagtagtttc agcaacagca tctgaatcag caccacttca atatatagca
    44041 ccatatgctg gagcagctat gggtgaagaa tttatgtaca atgggaaaca tgtacttata
    44101 gtgtatgatg atttaagtaa acaagctgtt gcatatagag aaatgtcatt gttacttaga
    44161 agaccaccag gacgtgaggc atatcctgga gatgtattct atttacattc aagattactt
    44221 gaaagagcag ctaagttatc tgatgaattg ggtggaggtt caatgactgc cttgcctata
    44281 atagaaactc aagctggtga cgtttctgca tatataccaa caaatgttat atctataaca
    44341 gatggtcaaa tatacttaca accagagtta ttctattcag gtgtaagacc agcagttgac
    44401 cctggtatat cagtatcaag ggttggtggt tctgcgcaaa ttaaagcaat gaaaaaagtt
    44461 gcaggaacat taaaacttgc atattcacaa tatagagaac ttgcagcatt ctcacagttt
    44521 ggttctgact tagatgaaga tactaaaaag agacttgctc aaggtgaaag aatcgttgag
    44581 atattaaaac aaggtgagca tcaaccaata aaagttgaaa atcaagttat gataatatat
    44641 gctgttataa ataatcattt agaggatatt ccgatagata atatagctag atttgaatca
    44701 gaattgtatg catttgtaga taataattat ccggaaatat caagaaaaat attaggtgga
    44761 gaagacttca ctcatgattt aacagatgca ataaatgaat ttaaagaaaa gtttgttgtt
    44821 gaagtataat cctttttaaa aaaggaggta aattaattgg caggagctgg aatcaaagca
    44881 atcaaaacac gtatgggaac tgttgaaagt acaaagcaaa taactaaagc aatggaatta
    44941 gttgcatctt ctaagcttag aaaagcaaaa gaaaaggctg aagattcaag agaatatttt
    45001 aatactttat acaatacagt aaaagatatt gcccaaacaa cacgtggaat tagaaatgaa
    45061 tttttaaaag aaagagaagt aaaaaataaa tgctatgtag tagtagctgg agacagagga
    45121 cttgcaggtg gatacaattc taatgtatta aaggaatctg tctctcatat gaaaggtaaa
    45181 aaggaaacag tgattactgt tggaaagaaa gcacatgaat ttttttctaa aagaaactac
    45241 aatatagcaa aagatataaa tagtgttgaa gcttgtggat atgaagatgc gacagaaatt
    45301 tcacaaacta ttattgacct gtataaaaaa ggtgaagtag atgaagttta tattgcttat
    45361 acaaaattta aatctgcttt atcacaagaa gttgaaattt tgaaattgct tccattagtc
    45421 tttgaaaaag atacagacga acaaggaatg gcagtaaaag aagaaaaaca aaatagatca
    45481 aaagtacaat atataccatc agcagaagca gttttaagtt atattgtacc tcagtttata
    45541 tcaggtacag tatatggtgg aattttggaa tcttttgcat cagagcaagg tgctagaaga
    45601 actgcaatgg aatcagccac agacaatgcg aatgaaatgc tatctaattt agagttaagt
    45661 tataacagag caagacagtc agctgtaact caagaaataa cagagatagt tggtggagtt
    45721 gaagctctaa aatagggagg ttaggaaatg gcaaatgtag gtaaagtagt acagattgta
    45781 ggagctgtac tagatgttaa gtttgatagt gaacaaagcc tacctaactt attaaatgcg
    45841 ttagtaataa agttaggaga taaagaaata gttgcagaag ttgctcaaca tataggtgat
    45901 gatactgtaa gatgtatagc tatgagtgca acagacggac ttgtaagagg tatggaagtt
    45961 gtagatacag gagggccaat aagtgttcct gttggagatg aaactttagg tagaatattc
    46021 aatgttcttg gaaaaccagt tgatgggaaa ccagctccaa aatctgcacc taaattacct
    46081 atacatagac cagcaccagc atatgatgaa ctagaaacta cagcagagat actagaaact
    46141 ggtattaagg tagttgactt actagctcca tatctaaaag gtggaaaaat aggtctattt
    46201 ggaggagcag gagtaggaaa gacagttcta atacaagagc ttataaacaa tattgctaag
    46261 caacatggtg gtatttctgt gttttcagga gtaggagaaa gaacaagaga aggtaacgac
    46321 ctttatggcg aaatgagtga gtctggagtt ataaataaaa cagctctagt atttggtcaa
    46381 atgaatgaac cacctggagc gagaatgaga gttgctttaa ctggacttac aatggcagaa
    46441 cattttagag atgagcaagg acaagacgtt ttacttttcg ttgataatat attccgtttc
    46501 acacaagctg gttcagaagt ttcagcactt ctaggacgta tgccatcagc tgttggttat
    46561 cagccaacat tagctactga aatgggtgca cttcaagaga gaataacatc aactaagaaa
    46621 ggttcaataa catctgttca agcagtatat gtaccagcag atgacttaac tgacccagca
    46681 ccagctacaa cattctctca cttagatgca aaaacagttt tatcaagaca aatatcatca
    46741 ctgggtatat atccagcagt tgaccctctt gaatctacat caaggattct tgaccctagt
    46801 atagtaggaa aagaacatta tgaagtagct agaggtgtac aatcaatact tcaaagatat
    46861 aaagaacttc aagatataat tgctatactt ggtatggatg aattatctga tgaagataag
    46921 ttaatagttg cacgtgctag aaagatacaa agattcttat ctcaatcatt cacagttgct
    46981 gagcaattta caggaaatcc aggtcaatat gttcctgtta aagagactgt aagaggattt
    47041 aaagaaattc ttgaagggaa acatgatgac ttaccagagt cagcattctt atttgtagga
    47101 acaatagaag acgctgttag aaaagcgaag ggaagtatgt agtcatggcc agtgaatttt
    47161 cattgaaagt tgtaactcca agtaatactt tttatgatgg agatgttgaa atgatagtag
    47221 ttagaactac aaaaggagac aaaggggtgt tgaaaggtca tataccattt gtttcaggta
    47281 tacctgtagg aactttaaag ataaaaaaag agggaagttt taaagaggcg aaaatagcag
    47341 aaggattcat taatgtaact caagataaaa ctgtcatatt gacagaatct gctgagtgga
    47401 tttaataaat taatttagag tataaaaagt cattttgacc aaaaaaactt ttgggtaaaa
    47461 tggctttttt gcgttttggt gttaataaaa taacgataaa attgtaagtt tttagtgata
    47521 ataaattcac aaaatgttta aatttactgc caaattatgt tgtaattaat aagataaaga
    47581 agatttagat agcttttatg taagtgaaaa agtcttcata taagaaatac atataaaatt
    47641 tttataaaat ttaaaaaatt tgcttagttg tgtagtataa taaataatat aatagatttg
    47701 tatataatat tcattacaaa taattttaaa agttacagtt ttattataat gattattaaa
    47761 ttaagataag taacataaac taaataataa tgtgtattat cgatagtaaa tttataaatt
    47821 cattattaat tattgaaaga agtattataa attttctgga ggaacaatga atatatttga
    47881 tattggcgta gatataattg aaattgatag aataagaaag gctgtagaca aaaataatag
    47941 gtttttggag aagatattta cagatagaga aatagagtat tttaattcta agaattttaa
    48001 agcagaaagt atagcaggta atttcgcagc caaggaagct ataagtaaat ctataggtac
    48061 aggaattaga ttgtttaatt ttaaagatat agaagttctc agaaatgaaa tgggaaaacc
    48121 aatagtaaaa acttataata atctagcaaa aatgtgcata gattataatg ttttagaaat
    48181 aaaagtttca atatcacatt caaaagatta tgctatagca aatgcgataa ccataattaa
    48241 ggactaagga gtgattacat tatgatggat aaaacagcaa aagaaataat gacaacagat
    48301 gtaatagtag caaaacaaga tgatagtata gcagatgtag ctaatatgct aattgcagaa
    48361 aaaataggag gtctgccagt tgtagattca gagaatagag ttgttggaat aatttctgaa
    48421 acagatattc ttaaaaaaga aaaatatata gaagcacctc tctatataaa tctattacaa
    48481 ggattgattt tcttagatga cttaaaaaaa gttgaaaaag atataaaaca agtagctgct
    48541 tataaggttg gagaacttat gtctaaagat ataataaagg ttcatgaaga tgataagttt
    48601 gatgatgtag ctaatgtaat gattaaaaaa tcaataaaca gagttcctgt agtagatgat
    48661 gacaacaaac ttaaaggtat aatttgtaga tatgatataa taaaagcttt atacaatgaa
    48721 taaactgaag taggaggtgt gatgtgagaa aaaagtggac catagtatgt attatgttct
    48781 tggcactggt tataattgta ataggatgcc aaaaacgaca gtccacaaaa gaagaagtat
    48841 acaaagattt tcaaaagcag atttctgata tgaattatta cagttgtaaa gctgaagtag
    48901 aagtagttgg aaataaaagc ccacacaatt atgttttaat tcatacttat aaaaaaaccg
    48961 ataactataa attagaagta atttccccaa aacatttaaa agggaaaagt atagaatatc
    49021 aagatgataa aatattagtt aaaaatccaa agattagtga tgtagtggag ttacctaata
    49081 caggaaaaaa taatcaatat ctttttgtag gagactttat aaaaaactat cttcaaaatg
    49141 aggagatgaa agtaaagtta tccaaaggac atttggtttt agagactttt attcctgggg
    49201 ataataaata ttttaacaag caagtattat atgtaaatgc tgacacaaaa aatcctgaaa
    49261 aaatggaagt gttagataaa gagggagtgc caagatttac agtaaaatac aaagattttg
    49321 aatacagaaa ctaaggggga ctaaagacat gcaaaaaata acagtgccta catgggcaga
    49381 gataaatcta gataacttaa gatttaactt aaataatatt aaaaatttat tagaagaaga
    49441 tattaagatt tgtggagtaa taaaagctga tgcatatgga catggtgcag tagaagttgc
    49501 aaaattgcta gaaaaagaaa aagtagatta cttagcagta gcaagaactg ctgaaggaat
    49561 tgaacttaga caaaatggca taacacttcc tattttgaac ttgggatata ctccagacga
    49621 agcttttgaa gattctataa aaaataaaat aactatgaca gtttattctt tagaaacagc
    49681 acaaaagata aatgaaattg caaaatcttt aggagaaaaa gcctgtgttc atgttaaaat
    49741 agactcaggg atgactagaa taggtttcca acctaatgag aagtcagtac aggaaataat
    49801 agaattaaat aaattagaat atatagattt agaaggtatg tttactcatt ttgctacagc
    49861 tgatgaagta agtaaagagt acacttataa acaagctaat aattataaat ttatgtctga
    49921 taaattagat gaggctggtg taaaaatagc tataaaacat gtatcaaaca gtgcagctat
    49981 tatggattgc cctgatttaa gattaaatat ggtaagagca ggaataatat tatatggtca
    50041 ttatccatct gatgatgtat ttaaagatag attagaatta agaccagcca tgaaattaaa
    50101 atcaaaaatc ggacatataa aacaagttga accaggtgta ggaataagtt atggactaaa
    50161 atacacaact acaggtaaag aaacaatagc tacagttcca ataggatacg cagatggatt
    50221 tactagaatc caaaaaaatc caaaggttct tattaaggga gaagtgtttg atgtagttgg
    50281 tagaatatgt atggatcaaa taatggttag aattgacaaa gatatagaca taaaagttgg
    50341 agatgaggtt atactatttg gagaaggcga agttacagct gagcgtatag ctaaagactt
    50401 aggaactata aactatgaag tgttatgtat gatatcaaga agagttgacc gtgtttatat
    50461 ggaaaataat gagcttgtac aaataaacag ttatttgcta aaataaaacc atgatattat
    50521 aatctgggag gcgattttgt gtacgataag agtaaagaaa aaatagaagt tactttacct
    50581 gactctctga tttctgaagt tgatagtata gttcagatgg agaacagtaa cagagaagat
    50641 tttgcaaaag ctgcttttca gttctatata actcagaaaa agaaaattca tattaaagaa
    50701 tctatgaaaa cgggttatag agaaatgggt caaattaatc tgtcactagc tgaattaggt
    50761 atgacagttg aagaagtatc ttctgatgat aaaaatgaag ggaagattgc ccaaggtgag
    50821 tactaattgt gaaataaagc gtggtgattt atattacgct gatttaagtc cagttgttgg
    50881 ttcagagcag ggaggcgtta gacctgttct gataatacaa aatgatgtag gtaataaata
    50941 tagtcctaca gttatagtat cagctataac atctcagatt aataaagcca aattacctac
    51001 tcatgtagag ataagttcca atgaatatgg gcttaataaa gactcagtaa ttcttttaga
    51061 gcaaattaga actatcgata aaaagagact gagggaaaaa attggttgtc tagatgaaaa
    51121 tatgatgata aaagtagatg atggacttca aataagttta ggattgttta ctttttaaag
    51181 agggatttgt cttttattag acaatccctt ttttctttta aatatctctt aaggtttaat
    51241 tttaatgtac tatgttaata aaattaaaaa taacgcaaaa aaatagcata aaagtgatat
    51301 aataatatgg aattgatata aaattacgaa ctcaagggag gtaattgcat gagagaccat
    51361 aaaggattaa atgagattgc gcgtatcata agaagagata tagtttcaat gatacataga
    51421 gccaaatctg gacatccagg agggtcatta tcagtagttg aaatacttac tgcgttatac
    51481 tttgatgaaa tgaatgtgga ttcttcaaat cctaaaatgg aggatagaga tagatttgta
    51541 ttatcaaaag gacatgctgc accagcatta tatgctacat tggctgaaaa aggctatttt
    51601 gataaggaag aactgaatgg attaaggaag ataggaagga tgttacaagg tcatccagac
    51661 atgaaaggca cgccaggtgt ggaaatatct actggttctt taggtcaagg tttttcagtt
    51721 gcatgtggta tggctatggc atcaaagttg gataatgctc cgtggaatgt gtatactttg
    51781 cttggtgatg gtgaggttca agaaggaatt gtttgggaag ctgctatgag tgctgcacat
    51841 tataaacttg ataatttaat tgctttttta gataataatg gacttcaaat agatggagat
    51901 atagaaagcg taatgagttt aggttcaata gttgataaat ttaaagcttt tggttggaat
    51961 gtaattgaga tagatggaca tgattttgac caaatatttg cggctcttga tatagctaaa
    52021 tcaacagtta aaaaacctac aatgatagtt gcaaaaacta taaaaggcaa aggaatatct
    52081 tttatggaaa atcaagcagg atggcatggg acagctccaa gtgatgagga gctagaaaaa
    52141 gctttgcttg aattaggagg tgctgacaat gagtaaaatg gcgacgagag aagcatatgg
    52201 taaagcatta gtaaaattag gtcaaataaa tgacaatgtt gtagtattag atgcagattt
    52261 atcaaagtct acaaaaacac atgattttta caagtcattt ccagatagat tttttaatat
    52321 gggtatagct gaacaaaatt taataggcgc tgcttgtgga ttatcaactg ctggtaaaat
    52381 accttttgca agtacttttg ctatgtttgc tacgggaagg gcttttgaga ttataagaaa
    52441 ttcagtttgt tatccaaagc taaatgtaaa aatatgtgct actcatgcag gtcttactgt
    52501 tggagaggat ggagcatctc atgaaagtgt ggaagatatt gctataatga gagctatacc
    52561 aaatatgaca gttcttgttc cagctgatgg agttgaaaca gaaaaaatta tttttgaaat
    52621 agctaagtat aatggacctg tttatgttag attagggaga agttcagtac cagttttatt
    52681 tgatgaagat tataaatttg aaataggaaa aggtacagtc ttaagagaag gaaaggatgt
    52741 atcaattata gcttgtggaa ttatggtaaa tgaagctcta ttagcacaag aaaagttgca
    52801 agaagaaggt ataagtgcta gagttataaa catgtcctcg ataaaaccta tagacaaaga
    52861 tttaatatta gaatcagcaa aagaaacgaa tgctattgta actgtagaag aacatagcat
    52921 aattggggga ttaggttctg ctgtaagtga ggtagtagga gagtcttgtc caaccattgt
    52981 aaagaaagtt ggaattaaag atacatttgg agaatctggt actccaaatg aattgttaaa
    53041 aaaatatgaa ttaacttgtg atgacataat aaagactgtt aaagaagtta ttattgcaaa
    53101 aagaatgtaa gagatataaa ttattcatgt ttgttaataa tataatatat ttaaaagaga
    53161 ggtataaagt gttatattaa tttatatctc tctttttttt atatgatatc aatatatcaa
    53221 aaatgtaata aaatattcaa atttttacaa aaatagagaa aatattcaaa tatatattga
    53281 ctattcagaa aaatatgact aatatataat ttatagagaa actaatttta tatataatga
    53341 ctacttagat aaaagaatct aaaaaaatta tgagaggagg agtagggtat aaaatcagat
    53401 tataagtttt aagttattat actaaaaagc tataaaacag ttatcatgta ttttatgtta
    53461 aaggaaattg taggtagttt atcgaattgt ttaatcagaa gaaaaaattt cacatgggga
    53521 ggtaactgag atatgtcaga aaaagtaaat gttaaaacag tcatcagttt cgcaggtgcg
    53581 tatgtagcaa cagtaatcgg ttctggattt gcaacaggac aggaaataat gcaattcttc
    53641 tctttttatg gttttgcagg tattattggt gggataattt caatggtatt attctcatgg
    53701 atgggtgctt cagttatgtc aaaaggtaaa gagcttcaac ttaaagaacc tatcaaaatt
    53761 tatcgtgttt attgtggtaa gtatttagga atattctttg aatggtttgg acctttattc
    53821 ttatttggtg ttttcgtagt aatgatttca ggagcaggag caactttaac tgaatactat
    53881 ggattaaatc catttgtagg tagagtaggt atggcagtag tagcattatt aactgtttca
    53941 ctaggtcttg ataaattatc aaagatacta ggtggaatag ggcctataat aataatcttt
    54001 acacttttag ttggtggtat aagtttagct aaaaacatag gtaatatagg agaagctgca
    54061 caagttctta gcactgtaaa gatagctaaa ccagttccaa atgcctatct atctggtgta
    54121 atatatacta cttataacac aattgttgtt atggcattct taactggatt aggagcaagt
    54181 gctgctaaca aaaaagaagc tatttgtggt ggtatattag gtggagtagc acttatggct
    54241 gcagctataa tgatgcactt agctattctt tctgatatag gaaacttata ttcaaaagca
    54301 ataccatcat tattcttagc agataagatt tcaccagtaa ttggagtttt attctcaata
    54361 attcttatat taggtatata tacaacagct gtacctttat tatggtcagt aactaataga
    54421 tttgtagatg ataaacatcc taagtttaaa ttaataactt taatagcagc tatattaggt
    54481 cttataggtg gcttcctacc atttgataag ctggttggta ttttatatcc atatactgga
    54541 tatatgggcg tcataatact ggtttgtgtt ctttatagac agattacaaa aacatctgga
    54601 tatcaagaag gaatcagtga caaataagta ttttttaata ttgtaaactc aagagctata
    54661 tgaaaacaat gaaaatgttt tcatatagct ctttttattt tttagctcgt ttattatgaa
    54721 atagaaaaaa catgtttttt agttacatta catatattta taattatgta taaatatata
    54781 ctaatcaata atttagagtc taagataagt ttataaatca tatatttaga atatatggga
    54841 ggagtgataa gattgaatgt aaagtttaat attaaaggta taatttatgg agttatagct
    54901 cttgtattcg ttataggggg atttatattc ataccaagta tgtttgtaga gaaatctaaa
    54961 cccatagatt atacagtatt acaaagaaat tcaataccag aaaagatatt agatgtgatg
    55021 gataagtata caaatgaaga aagagcatta gcggctaaaa tcgataacaa aatatacatt
    55081 atagtgacta gaggaaataa taaatatggt atagaaatgg acaaaataga aagtgtggtt
    55141 gaggaaggaa aggatgtttt gagagtcaag ataaagtata aagataagga agattctttt
    55201 ccatacatag ttgttgaaac aaatatgagt gaacttccag atagaataga attaaatgcg
    55261 acgaaataag aaggtatttt ataattaaat aactgcatat tgtacaataa agtcatggtt
    55321 tttattaatg taagtaaatt gtatatggtt cttattgttg taaaagccat gacttaaaat
    55381 gcttataaag taagatatta acagtagtat ttattactat attaaatggg gtctggaaat
    55441 acttaatatt ctaattattt ggagtgataa ttctagtttc agtaactatg tagtctaatt
    55501 gtgcatcatg agtttctttt ggaatagaat cgaatatctg gaagtcaaat gctatgccga
    55561 tagttatagc atcttttctt aagttttcta aaaatctatc ataaaatcct ccaccatatc
    55621 ctaatctata gcaatgtata tcatatgcta cagctggtac aattacaaca tctaaggcct
    55681 taatatcaat tgtaggtgat ttttcgttag gttctcttat tccatatgct ccaattctca
    55741 atccctcttt taaatctgtt atttgagtag ggattaatgt atgattttct tttattgtga
    55801 tgggacttga tactattttc tttagagaca aaagtttttt gattaaatta tctgtttgaa
    55861 cttcattgtt aaaatttaga taaagcatta tattagttgc atttcttata tcttccattt
    55921 gaagtaattt ttcagtgatt aaatttgaat tactaagtac gaagtttgca ctcttatttt
    55981 ttcttgattc aataactttt tttctaaatt cttttttcat aaaataattc ctcccttaat
    56041 atgttataat gtgtacagca attgtattta atataaattt tagtataatt tattgtaata
    56101 aagcaatata tttgggtaaa tgtaataaaa ataacagcta gtcatataat aataatagtt
    56161 tatgtaaaag gagatgtatc ttgtgatttc aaagaagaaa gctatttttt taggggtgat
    56221 tttagtaatt ataacagcta tggttacatc agcatttcag ttgacattgg ggaataaagt
    56281 tgtaatatcg aaagaactct atgaagacta taaaaaatat gataagttgt taggactaga
    56341 atctattata aaacaagatt tttataaaaa agtatcagat actgatttgg taaatggagc
    56401 agcaaaaggt ctatttctag gaacaaatga taagtattct ggttattata ctaaagatga
    56461 aatggagaat ctaattaatg atagtgaagg ttcttatgtt ggtgtaggta tgtatatagg
    56521 ggcatcaaaa gatggtggct tagtagtagt tcccatgaaa gattctccag cagagaaagc
    56581 aggtgtaaaa tcaggggata aattagtaaa agtcaatgga aaatctgtct catataaaaa
    56641 ttcagatgaa gctgttcgta tgatgaaggg taaaaaaggt aaaacagttg aactgactat
    56701 attaagagaa gataaacaat tgaactttaa agtaaaaaca gaccagataa ttgagaagag
    56761 tatagaaagt aaagtaattg ataatgattt aggatatata gaaattaccc aatttatatc
    56821 aagtacatat actgactttg ataaagcttt aaaagaatta aaagcaaaaa atatcaaggg
    56881 attagtaata gaccttagaa ataatcctgg tggaatgcta gatatatgta aagaagttgc
    56941 tgatgagtta attggtgaag gaactatagt atatacaaaa gataataagg gaaatactga
    57001 gtacttgaaa tctgataaag aaaaattggg acttccaata gtagtgttaa ctaatggaga
    57061 aagtgcttct gctgctgaaa tattaacagc tgcaatcgtt gataataaag aaggtatttc
    57121 tgtagggact acaacttttg gtaaagggtt ggtacaatca gttgtaagat tgaaggatgg
    57181 aacaggatac aagttaacta ctgctcaata ttttacacct aatggagatt atatcaatga
    57241 aaaaggtata aagcctacta ttgaagaaaa agatgaaaat aaacagttag atgtagcaac
    57301 taagtggctt agagaacaga ttgataaata ggatttaatt tttataaact tatactataa
    57361 aataaagaac attataaaag agatgtctca aaatgaactt tttagttcat gagggagtct
    57421 ggaaatattt atgtgtattc tttaaataaa aatttatttt ttaagtgata attaaatttt
    57481 tacagacagg gctatggttt taaaaatcat agccttattt tgatattttt taaaaacaca
    57541 ttgcaaccca gttgttccaa atattatatc atttactttt aaaatatcgt aaactgcata
    57601 ct
//
LOCUS       DAYAOY010000014        54985 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYAOY010000014 DAYAOY010000000
VERSION     DAYAOY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.014"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            219..1061
                     /locus_tag="ACGI6B_000941"
     CDS             219..1061
                     /locus_tag="ACGI6B_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFG9750406.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            1076..1540
                     /locus_tag="ACGI6B_000942"
     CDS             1076..1540
                     /locus_tag="ACGI6B_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750407.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1554..2699
                     /locus_tag="ACGI6B_000943"
     CDS             1554..2699
                     /locus_tag="ACGI6B_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9750408.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2743..3678
                     /locus_tag="ACGI6B_000944"
     CDS             2743..3678
                     /locus_tag="ACGI6B_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HFG9750409.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3952..6645)
                     /locus_tag="ACGI6B_000945"
     CDS             complement(3952..6645)
                     /locus_tag="ACGI6B_000945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFG9750410.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6859..7557
                     /gene="yunB"
                     /locus_tag="ACGI6B_000946"
     CDS             6859..7557
                     /gene="yunB"
                     /locus_tag="ACGI6B_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HFG9750411.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7769..8776)
                     /locus_tag="ACGI6B_000947"
     CDS             complement(7769..8776)
                     /locus_tag="ACGI6B_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFG9750412.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            9004..9888
                     /locus_tag="ACGI6B_000948"
     CDS             9004..9888
                     /locus_tag="ACGI6B_000948"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9750413.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            10090..11640
                     /locus_tag="ACGI6B_000949"
     CDS             10090..11640
                     /locus_tag="ACGI6B_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9750414.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11669..12316
                     /locus_tag="ACGI6B_000950"
     CDS             11669..12316
                     /locus_tag="ACGI6B_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFG9750415.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12458..12718)
                     /locus_tag="ACGI6B_000951"
     CDS             complement(12458..12718)
                     /locus_tag="ACGI6B_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HFG9750416.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12935..14218
                     /gene="hflX"
                     /locus_tag="ACGI6B_000952"
     CDS             12935..14218
                     /gene="hflX"
                     /locus_tag="ACGI6B_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFG9750417.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14226..14903
                     /locus_tag="ACGI6B_000953"
     CDS             14226..14903
                     /locus_tag="ACGI6B_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFG9750418.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14989..15432
                     /locus_tag="ACGI6B_000954"
     CDS             14989..15432
                     /locus_tag="ACGI6B_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9750419.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15737..16381
                     /locus_tag="ACGI6B_000955"
     CDS             15737..16381
                     /locus_tag="ACGI6B_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFG9750420.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16467..16844)
                     /locus_tag="ACGI6B_000956"
     CDS             complement(16467..16844)
                     /locus_tag="ACGI6B_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFG9750421.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16844..17230)
                     /locus_tag="ACGI6B_000957"
     CDS             complement(16844..17230)
                     /locus_tag="ACGI6B_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFG9750422.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17409..18164
                     /gene="nadE"
                     /locus_tag="ACGI6B_000958"
     CDS             17409..18164
                     /gene="nadE"
                     /locus_tag="ACGI6B_000958"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFG9750423.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18247..18966
                     /locus_tag="ACGI6B_000959"
     CDS             18247..18966
                     /locus_tag="ACGI6B_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9750424.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19133..20137)
                     /locus_tag="ACGI6B_000960"
     CDS             complement(19133..20137)
                     /locus_tag="ACGI6B_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9750425.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20403..21299
                     /locus_tag="ACGI6B_000961"
     CDS             20403..21299
                     /locus_tag="ACGI6B_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFG9750426.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            21420..22682
                     /locus_tag="ACGI6B_000962"
     CDS             21420..22682
                     /locus_tag="ACGI6B_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFG9750427.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22758..23951
                     /locus_tag="ACGI6B_000963"
     CDS             22758..23951
                     /locus_tag="ACGI6B_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFG9750428.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24580..25374
                     /locus_tag="ACGI6B_000964"
     CDS             24580..25374
                     /locus_tag="ACGI6B_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFG9750429.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25469..26809
                     /locus_tag="ACGI6B_000965"
     CDS             25469..26809
                     /locus_tag="ACGI6B_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9750430.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26946..28178
                     /locus_tag="ACGI6B_000966"
     CDS             26946..28178
                     /locus_tag="ACGI6B_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFG9750431.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            28218..29360
                     /gene="acrC"
                     /locus_tag="ACGI6B_000967"
     CDS             28218..29360
                     /gene="acrC"
                     /locus_tag="ACGI6B_000967"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HFG9750432.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29371..30165
                     /locus_tag="ACGI6B_000968"
     CDS             29371..30165
                     /locus_tag="ACGI6B_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFG9750433.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30179..31198
                     /locus_tag="ACGI6B_000969"
     CDS             30179..31198
                     /locus_tag="ACGI6B_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9750434.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     VRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            31297..32673
                     /locus_tag="ACGI6B_000970"
     CDS             31297..32673
                     /locus_tag="ACGI6B_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9750435.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(33005..33349)
                     /locus_tag="ACGI6B_000971"
     CDS             complement(33005..33349)
                     /locus_tag="ACGI6B_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750436.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            33837..34847
                     /locus_tag="ACGI6B_000972"
     CDS             33837..34847
                     /locus_tag="ACGI6B_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9750437.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            34983..35471
                     /locus_tag="ACGI6B_000973"
     CDS             34983..35471
                     /locus_tag="ACGI6B_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9750438.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            35647..37575
                     /locus_tag="ACGI6B_000974"
     CDS             35647..37575
                     /locus_tag="ACGI6B_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9750439.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            37752..38162
                     /locus_tag="ACGI6B_000975"
     CDS             37752..38162
                     /locus_tag="ACGI6B_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFG9750440.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            38184..38486
                     /locus_tag="ACGI6B_000976"
     CDS             38184..38486
                     /locus_tag="ACGI6B_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HFG9750441.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(38703..39395)
                     /locus_tag="ACGI6B_000977"
     CDS             complement(38703..39395)
                     /locus_tag="ACGI6B_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFG9750442.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(39421..40608)
                     /locus_tag="ACGI6B_000978"
     CDS             complement(39421..40608)
                     /locus_tag="ACGI6B_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9750443.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            40944..42821
                     /locus_tag="ACGI6B_000979"
     CDS             40944..42821
                     /locus_tag="ACGI6B_000979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9750444.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            43178..44584
                     /locus_tag="ACGI6B_000980"
     CDS             43178..44584
                     /locus_tag="ACGI6B_000980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9750445.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            44612..45460
                     /locus_tag="ACGI6B_000981"
     CDS             44612..45460
                     /locus_tag="ACGI6B_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9750446.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            46121..46813
                     /locus_tag="ACGI6B_000982"
     CDS             46121..46813
                     /locus_tag="ACGI6B_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750447.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            46810..47745
                     /locus_tag="ACGI6B_000983"
     CDS             46810..47745
                     /locus_tag="ACGI6B_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9750448.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            47893..48816
                     /locus_tag="ACGI6B_000984"
     CDS             47893..48816
                     /locus_tag="ACGI6B_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750449.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            48800..49570
                     /locus_tag="ACGI6B_000985"
     CDS             48800..49570
                     /locus_tag="ACGI6B_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750450.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            49580..50362
                     /locus_tag="ACGI6B_000986"
     CDS             49580..50362
                     /locus_tag="ACGI6B_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750451.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            50739..51275
                     /gene="rbr"
                     /locus_tag="ACGI6B_000987"
     CDS             50739..51275
                     /gene="rbr"
                     /locus_tag="ACGI6B_000987"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9750452.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            51307..51696
                     /locus_tag="ACGI6B_000988"
     CDS             51307..51696
                     /locus_tag="ACGI6B_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFG9750453.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            51733..52119
                     /locus_tag="ACGI6B_000989"
     CDS             51733..52119
                     /locus_tag="ACGI6B_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFG9750454.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            52246..53688
                     /locus_tag="ACGI6B_000990"
     CDS             52246..53688
                     /locus_tag="ACGI6B_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFG9750455.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            53985..54800
                     /locus_tag="ACGI6B_000991"
     CDS             53985..54800
                     /locus_tag="ACGI6B_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9750456.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        20362 a         6597 c        10065 g        17961 t
ORIGIN      
        1 ttattcaatt atttgaaaat ccgttatttt aacaatataa attaataaat tatttgtatt
       61 ttatatcatg aatctataat atttttttga ttcataacta aaagtatata aaaatgatga
      121 ataaatatat attatacatt atcaatgata ggttatataa taaaatcact aacaattata
      181 ttataagaaa attataaaaa ttacaaggag gtatagaaat gaaagaaaaa gcagggatta
      241 gaaatgccat aatagcagcc tttataatca caatattggc agaattaatt ggacctattt
      301 catttaaagt tatgggaatt aatgtaactt tacttagtat actatgggct atattcatag
      361 gaatggctgt ttctcctcat ttattaggga gagttatacc agccttaaaa aagtttatag
      421 gggataatga aataaatgta tctccatatt tattatcttt gacactatat ccattaggga
      481 taatgtttgg aattaatgca gggcctaaag ttggtatatt acttcaagca ggaccagcac
      541 ttttattaca agaatttggt catatgggta caatgttgat agcattacct gttggagtag
      601 cattaggtct tggaagaagt gcattaggtg gtactttttc attatgtcgt gacacagcac
      661 taggaattat tggagataag tatggattaa actcaccaga aggtatagga acactaggaa
      721 cttacataag tggtagtata tttggaacat tattattttc atttttagca ccagtgggtg
      781 tacttttagg atttcatcca tatgctttag caatggcatc aggaatgggt agtggttcaa
      841 tgatgggagc tgcaacagca tcattaatga atacagttcc acaaatgtca gagcagattt
      901 tagcatattc agctactagt ggattgatta cagctgttac tggaatatat atagaattat
      961 tcttatcttt accagtagcc aactactatt atagcaaagt agctccagtt attgaaagat
     1021 ttagaggaag aaaaacaaga agaacaagta ataaattgta aaggaggata tatatatgga
     1081 aaaaactttt aagactatta tggagcagat aaaaatagta ttgtttatat atattattat
     1141 ttgctttgga caaatgatag cacttaagac tcaggctcaa gatttgttga ttggaagtgt
     1201 atctggattt ttgttggtga taattgctat tataataaag aattttgtta aaaaacctaa
     1261 tttaccaggt tttgcatggg ctacattagt tgcatttgtt ttatcgcttc caatatctcc
     1321 agtgggggat atcatagcaa caaatgtagg gaaaatcaat tttatgtcta cagtaacacc
     1381 attattagct tttgcaggaa tctctgtagg taatcaatta gatatattaa agactttgag
     1441 ttggaagtta gtagttatat catttatagt tatgacatct acttactttg gttctgcttt
     1501 tatatctcaa acagttttaa gattaaatgg aatgctttaa ggaggtagta aatatgaatg
     1561 aattaaaagc aaaaattaaa gaggaaataa agttattatc tgaagagaaa aagacatcat
     1621 ttgaaaaagt ttctgactat ttattttcaa atccagaatt agcatttgag gagtataagt
     1681 cacaaaaagc attatgtgat ttgttagaag aaaatgaatt taatgtaact aaaggtgtag
     1741 gaggattaga aacatctttt gaagctgtgt attcaaatgg aactaatggg aaaacagttg
     1801 cttttttagc tgaatatgat gcacttccag gaatgggaca tgcatgtggg cacaatatca
     1861 tagggacaag ttctgttggt gcaggtatca tattaaaaga aatcatgaaa aaacataata
     1921 tagaagggac tgtaaaggtt tttggaacac ctgcagaaga aagagtaggt ggaaaaataa
     1981 ctatgattaa agaaggtgta tttaataatg tagatgctgc attaatttta catccaagtg
     2041 atgcttcaat gcctgatgat atatcatttg ctcaagttaa tttaaaattt gattttactg
     2101 gtaaggcatc acatgctgct gcttttcctt gggaagggaa aagtgcctta agtggagtaa
     2161 tagcactatt taacagtgta aatagcatga gattacatct taaagattat gcaagagtac
     2221 atggaattat aactgatgga ggtagcatac ataatataat tcctgaaaaa tctacagcta
     2281 tatttaatgt aagagcctta agtatagaat atttaaatga gatttgtgaa atgcttaaaa
     2341 attgtgctaa aggagctgca atttctactg gaactaatgt agaaatagtt caattagatg
     2401 aaatatacaa agagattaag aatgattctg aattagtgaa tatagttaga caaaattttg
     2461 aagttttggg tgaagactat gttgaaagag atttaagtca aggtataggt tcaactgata
     2521 caggaaattt aactcatgag atacctgcaa tacaagcata tataaaatta aaagaaaata
     2581 ctgcaactca tacagatgaa tttgctgttg cagctggtgg tgaagaaggc agagtagcac
     2641 ttataaaagc aattaaggtg ttagcaatgt gtggagtaga tattttgtat agtaaataat
     2701 atataatttt aaaatatatt aagaaaggaa atcggattag acatgatatt aaaacaagta
     2761 atagaggttt atgacatttt agataaagca aatgctaatg gggaagaagt aaaaacttac
     2821 ttacaaggtt atggtgaagt agatgtaact gtaaaagaat tgtcttcaag taaaggttca
     2881 acagatttag taaagcttac aattcctgga aaaaatggaa aattaaaagg tggagatgct
     2941 cctactcttg gtattttagg tagactagga gggattggag caagaccaga agttataggt
     3001 tttgtctcag atggtgatgg agcattggta gcaattgcag tggctgctaa acttttggat
     3061 atgcaacgaa aaggggatat attagatggt gatgttgtaa tttcaactca tatttgccca
     3121 gatgctccaa ctaaagaaca ctatcctact ccttttatgg attctccaat tgatatgatg
     3181 actatgaatg aaaatgaagt tgatagtagt tgtgatgcaa tactttctat agatactact
     3241 aaaggaaata gagtaatcaa tactagagga tttgcaatat cgccaactgt taaagaaggc
     3301 tacatattaa aaactagtga taacctttta gatattatgc agactgtaac agggaaatct
     3361 ccatttgtat tccctttgtc tattcaagat ataacaccat atggaaataa tctgtatcac
     3421 ttaaatagta ttttacaacc agcagtagct acagatgcac caatagtagg agtagcaatt
     3481 acaacagaga tgcctgttgc tggatgtgct acaggtgcaa ctaattttag tgatttagat
     3541 tcagctggga gatttgcaat tgaagttgcc aagctatttg gtagaaataa gtgtgatttc
     3601 tatgataaag aagagtggga aatgcttatt aagagatatg gcaaattaaa tagatttcaa
     3661 acatttggta tacagtaatt agaaaaatat taagtttata taatttgtga tataactatt
     3721 taagaatata taaacaatta ggaataaaag taagaataaa gcacttagtt aaaaacttta
     3781 aaaataaaat atttatttgt ttatataaaa aggaggattc tggttattgg gattctcttt
     3841 tttatgaata caaataatat attgtaatta taaaaaaagg agcgtttcaa agtgattttt
     3901 ttaatcacct tgagacaact ccttatattt ttatttaaat tctatttttt attaagttgc
     3961 tggagttcca ccaccatcag gaggagtagt agagccacca ccattatttc caccaccatc
     4021 aggaggagta gtagagccac caccattgtt tccgccatta tcaggaggag tagtagagcc
     4081 accaccatta tttccgccat tatcaggagg agtagtagag ccaccaccat tgtttccgcc
     4141 attatcagga ggtgtagaac cgccaccatt atcacctgtg ttattattgt tattattatt
     4201 ttcttctggt tggttattgt ctggtttttt tgtatcttca gatgatggtt gagaacttac
     4261 tttagtagga gcagtacctt caataaatgc tgctttagag ccagaagatt gctttcctcc
     4321 atcctctaaa ttaatttttg taaaatatac atttttagga acttcaaatt cagtaaccgt
     4381 taaattagca tgtatttttt ccataatttt ttcccatagt tttgcagtac tagtacttcc
     4441 atgtggtact tctctacgtt ttatagtgtt accagaatca tcttttctac cagcatcatc
     4501 tccaatataa gttgcaccaa cataataagg agtgtaaccg acaaaccaag catcatatgc
     4561 actatttgta gtacctgttt tacctgctac aggcattccc tttggaagtg tagcaacacc
     4621 accagtacct tcagtaatta cgctttctaa catatctgta acaacataag ctacttcagg
     4681 gtctacaact ttatgttctt caggagcatt tttaactaat aattgaccat caaaggtagt
     4741 tatagtagta aaaatagaag gttctacata aataccgtga tttgccaatg taccataagc
     4801 agcagccatt tgaagtggag taataccatt cgccatacca cctagtgtca atgatgggaa
     4861 ttttctatca cttgcttcag atgaacctga gtgattattc tttaaagttg ttataccaaa
     4921 gttctttaag taatccatca ttatatctac acattcatca taagagtctc caagcatttc
     4981 agcagttttg acagctatcg tgtttgaaga tttagcaagt gctttacgta aactcataga
     5041 accatgacca gcagtagtag ttcttggatt ccatttatta ttttcttcaa acttatatcc
     5101 tcctcgagaa tcacttagag cagttgcttg tgttattttt agagtatcaa tagctggcgt
     5161 atatattgct aaaggtttta tagaagaacc tggctgatga gggttagtag cacgatttaa
     5221 agtttttcta ccacttatat ttcttcctcc agctagtgct cttatttcac catttttata
     5281 atctaagatt accattgcag cttgcggttg gctaactcca tcaggaccga ccatatgtcc
     5341 tgggaaattg ctatttctat caaattcttc ttctaaagca tcttgcattt tagggtctat
     5401 agttgtgttt acaattagac caccattgta gaataagttt tgagcttcat catttgtatg
     5461 tccttgttcc attaaagcat ttataacttc actttcgata aggtcttcta cagatgaaga
     5521 aactttatct gatgattgag gcaattttat ttctatttta gctttgattg cttcatcata
     5581 ttcactttgt tttatatatt tcaattctaa cattttcttt aaaacaattt cttgtctttt
     5641 tttagcttca ggattactta cagcctttct aacaactaat gtacctgctt ttaattgtct
     5701 ataagtatca ttagatataa gctcccaaga ttttattttt tcaatcatat caaagtccac
     5761 ttgagtaggg tcatctaaat catcatcagt tgtgttaaca aaaaataata atttattctc
     5821 taaatcctcc ttagtttcat ttccctctaa cttagaagtt ttataggctg aaaatcttga
     5881 tggattttta gttgaaccag caagaagtgc tgactctgct agagtaaggt cagctgcaga
     5941 cttatcaaaa taaccacgtg caccagcctc agcacctgct agacctctac caacgaagaa
     6001 gttatttaaa taagtttcta gtattttatc tttacttact gttttactca tctcatgtgc
     6061 atagtaaata tcctttattt tacgaggtat agtttgctgt tcagttgtta acagcatttt
     6121 ggatacctgc ataggaatag tactaccacc ttgcttagta cctgtaaggg tctttaatac
     6181 tgacctaaat aaacctttaa tatcgacacc cttatgctca taaaaacgct catcttctat
     6241 tgcaattaca gcatgttgaa gataagggga aatcttatct aatggaacgg gtgtcttttt
     6301 atttacactt ggagctgtag ataatgtttt tccattagcg tatttaatag tagttgtttg
     6361 gttcgtctgt ttatctaaaa cagcttctgt aactggagtt acatctctta gtgacgcaaa
     6421 tactagacca gcagtacctg ctgtaccaac aactagtaaa actaaaaata ctatcccaaa
     6481 tactcttagt tttttaaatt tatcgctttt tttagatttt ttcttgtttt ttgttgaacg
     6541 agtcttgtta gctgaagttc tattaattgg tttatttgta ttaccagatg agctaacttt
     6601 ttttctcctg attttatttc catttttatc attatcgtta ttcataagat tttaacctcc
     6661 ttacttaatg caatattata acacaaatag gagtagtttt gtaattaata gaaaaaatga
     6721 atttgctata atattctgta cattgtatat gtataaacgt aatatattaa aaagtctata
     6781 tttttatttt atttattttt gacaaatatt ataaaaatta tgccaaagct acatataaat
     6841 aaataggagg ggataatttt gaatagaata gtagatagaa aaacaaaaaa tgaatttaga
     6901 aaaatcatag ctattttttt agtaattctt ttcttgtctg tatttatagg cagttttata
     6961 tatatagata aaactttaag accgacaata acagttttag cagagactaa agctcttgaa
     7021 ctagcaaata ggtcgataaa taaagctgtt gctgagatgg tagaaggaaa aataaattat
     7081 gaagatttaa tggatataca acttgataat aatggtaaaa taactatgat acaagcaaac
     7141 actataatga tgaatgaaat tgcatctgca atagctttag aaatacaaga tgaattaaaa
     7201 aaggataaga cagcaagttc ttacatacct ataggaacag cattaggaag tcctatatta
     7261 gctaaatacg gtcctaaact tgaagtatct attgaaccta taggaacagt atcagtaaac
     7321 tttaaaacgg aatttgagtc ttcaggtata aatcaaacta gacatagaat atatttagaa
     7381 gctcaaacac aagttaaagt agtaatacca cttataactt caacaaaaca aattaaagct
     7441 caaataccta tttgtgaaac tataatagta ggtgatgttc cagaaagtta tgtaaatatt
     7501 ccagaaaaaa atctaggaaa tgttttacct aacactggaa agaataccaa caagtaatgt
     7561 aaagttgtat atttttaaat aaattcaaaa taatagttta taaataaaat ttttggaaat
     7621 aatattaata taattttaat aaaaatttat attttatact atgtaaaata tctaatatat
     7681 ttggtctgta ggttaaataa aagtaccttt agaccaaata tattattaat aacttaataa
     7741 catcttaaaa cttatttatt tatattattt acaacatcca tttaataaat acaccataac
     7801 ctacagctgg gtcgttttca attgcatgtg acactgctcc gattatttta ttatcttgaa
     7861 ctattggagt accactcata ccttgtacaa taccaccagt ttgagctaaa agctttttat
     7921 ctgtaatttt aattttaaaa cttttacttt ctggatgttt ttggttttct atatctataa
     7981 tttcaatatt atatttttta cacttattaa agttatcttg taaaattatt tgagcttttc
     8041 ctaatttaac ttcatctaaa gatgctactt tatatttttt taacttggaa atatcaaagt
     8101 tattaatttg acctcttatt ccaaaatcag tattttctgt aaagctacca ataatatttt
     8161 ctcgttttgc atttatacat ccaacactac ctttataaga tttttctata ttaaggtgct
     8221 tagttgtaga aattgaacct gttttaatag gtatttttct tgcattacca acacttattg
     8281 gatgacctac agctccaaac tctgatgtat ctggattgat atacgttaaa gtagcaaatc
     8341 cagaaagtaa attgttaaaa ctaatttttt ctaatatgtg tttggtagtt ttcattgtta
     8401 taagttgtcc acctctattt aataaaatag atacttgttt ttcttctggt aaagcattta
     8461 aggtattggc aaagtcacta tatccatcaa cttcagcatt atttaccttt actatagcat
     8521 cacccaatct aaatggagat gcttctccag gatttctaac tatgatattt tttaattctg
     8581 cgtcaatttg aagtacgttt ccaattggta ttaaatattc aggttcattt ttttgtaaat
     8641 tttgtgcata aattaaatta tttgagaaaa aatataataa aaataaaaat gttaaaacaa
     8701 ttgtaaattt tgacttagct aagttggttt ttttacttaa agtattattt ttaaaatgaa
     8761 aatttttaag ttgcataaaa cacctctttc ttattaaatt attttaataa tagaattgaa
     8821 gttttcttgc tgcttctata ttatgttgtg caagaatcat gttttgatgc ttggaaaact
     8881 agctcaataa caccttagtt ttcataaaaa taaataaaat aaatagtgta tatgataaaa
     8941 taaatacata ttcatataaa taaaaataaa caataactgt ctagcataag aaaggagcaa
     9001 aaaatgaaaa aatatagatt gttagttact tgtataattg ccatgggaat cataattata
     9061 ggaacatcaa atccagttag caatttttac aagactacaa atttgattag ttcaggaaat
     9121 atcacaaatg aaaaagcaga taaagatgaa aattcaaaca aaaataataa agataaaaat
     9181 gaagacaaat ctaattctaa aagtaataaa gataagtcat cagaggacaa atcgtcagat
     9241 aataaaaatg aaattcaaaa aaataaaaaa cttttaattt gtatagaccc agggcatcaa
     9301 ggaaaaggag atagcaattt agaaccagta gctccaggtt cttcctctaa aaaagcgaga
     9361 gtatcttcag gaacagaggg tattgctaca aaaaagccag aatatgtttt aaacttagaa
     9421 gcatctcttg ttttaaagag tatattagaa tctaaaggtt ataatgtaat aatgactaga
     9481 gagacacatg atgtaaatat aagtaattca gaaagagcaa tacttgctaa tgacaaaaaa
     9541 gcagatatgg ttgtaagaat acatgcagat agtttaaata attcttcaaa aactggagca
     9601 tctatattga ttcctgaaaa agatggaaaa tatacagctc ctatatatga agaaagtaat
     9661 aaatgtgctg aatttatcaa acagaatatg gaacaatcag gtatacaaat caatggaatc
     9721 gttcaaagag gtgatttgac tggattcaac tggtcaaaag taccagctgt attagtagaa
     9781 atgggattta tgagtaacta taatgaagac caaatgatgt caaatccaga ctaccaaaga
     9841 aaaatgatgc agtgtatagc tgatggattg gatgcgtatt ttaaataaga tatgaataga
     9901 gaaaaagtta ggttggtatt atttgcctaa tttttatctc tattttttat tgtacataac
     9961 acaaagaaga agtttatttt aggttaaatt gtcgtaaata taactaaaaa tattgggaat
    10021 acttgattta agtttaaaaa gaatatatta tagtatagaa tgtgtaaaat ttttttgaga
    10081 ggtgttgaaa tgctagtagt agaaaatgtg agtcatggat ttggtgcgag aacaatatta
    10141 gaaaatgtat cctttagact tagaaaaggt gaacatattg cactagttgg tgctaatgga
    10201 gaaggaaaat cgagtttttt aaatataata acaaagaagc ttatgccaga tgctggaaat
    10261 ataaaatggt cttcaagagc tacagtaggt tacttagacc aacatacggt attaagcaag
    10321 ggaaaaacta taagagaagt gcttagagag gcttttaagc atatgtttga cttagagcaa
    10381 gaaatgattg ctatgtatga caaaatggga gaagctagtg atgatgaaat gagtaagctt
    10441 cttgaagaaa cagcagagat acaaactata cttgaaaata gtggttttta tatgatagat
    10501 gcaaagatac aagaagttgc aaatggtctt ggtcttggag aaattggtct tgataaagat
    10561 gtaactgact taagtggagg gcaaagaaca aaagtacttc ttactaaact tctacttgaa
    10621 aatccaacta ttcttatatt ggatgagcct acaaactatt tagacgaaga gcatataaca
    10681 tggcttacta agtaccttca agagtatgag aatagttttg tcttagtttc tcatgatatt
    10741 gaatttataa ataatacatg taatgtaata tatcatatgg aaaatggaga gttaaataga
    10801 tataaaggaa attatgatga atttgtaaga cttaatgata taaagaaacg tcaagaagaa
    10861 caagcttatg ataaacaagt tgaagaaaga aaaagactag aggattttgt agctagaaat
    10921 aaagctagag tagcaactag aggaatggct aacagtaggc aaaagcaact tgataagatg
    10981 gaaatattag aaagaccaaa agaaaaaata aaacctactt ttgcttttaa agatgctaga
    11041 gcagctagta aaataatttt tgaaacagaa aatttagttt tagggtatga tgaagcattg
    11101 acaaaaccac ttaatttcca tttagaaaga ggaaaaaaga ttgccttaaa aggaatgaat
    11161 ggtattggaa aatctaccct attaaaaact ttacttggaa taataaaacc atttgaagga
    11221 aatgttaaat taggtgacta tttggaagtt ggatattttg aacaagagag ttcaagagaa
    11281 aatagtaata cacctatgga tgaagtttgg agtgaatttc cagggcttac aaactttgaa
    11341 gttagacagg ctcttgcaaa atgtggactt acaaatgaac atataacgag tcaaatgaga
    11401 gtattaagtg gtggagaagc tgctaaggtt agactttgca aagtaatgtt aaaaaatata
    11461 aatttcttag tattagatga gcctacaaat catttagatg ttgaggcaaa agatgaattg
    11521 aagaaggcta tcaaagagtt taagggaaca gttcttttag tatgtcatga gccagaattt
    11581 tattcagaaa ttgttgatga tgtatggaat atagaagatt ttacaactaa aatcgtgtag
    11641 tgatttaact tattgctggg gggatttcat gaaaaagttt ctaaatgtac ttttttcaat
    11701 ttgtattagt attgttataa tagtaggtgt tattaatttt actgttggat ttaaacaact
    11761 ttattatttt gacattgatt acttaaatat atcagagtta tctggtttat ctaaagatga
    11821 tataaaattg aactacgatt atctaattga ttataactta aataagaatg tgtctgagtt
    11881 caaattacca acattaaaat cttctccaca agggaagata cattttgaag aagtcaggaa
    11941 tatatttcaa aatataaata aattagctaa attattattg gtagtttctt tagtaggaat
    12001 tatattgagt gtaaaaaata aaaatataaa aatcttaaaa actacatcaa taacacttat
    12061 aattatgcca ttattattaa cagttccaat tttattaaat tttgaaaagt catttattat
    12121 ttttcataaa ttattattta gaaatgatta ttggatattt aatcccgatt tagacccagt
    12181 tataaatata ttgccagagg aatttttttt tcactctgga atgatgattt taatattaat
    12241 tttgttagta agtatagtac tgtttgtaat gtataaatta tgtaaatctt tatcaataaa
    12301 aatatttcaa tgttaaatag agggatattt ttatttccaa aataagataa taaaaaagga
    12361 ttagattaaa agttatacaa taaaaatagc tgtgcaataa tatttagact atgaaagtag
    12421 ttgaaaaata tttatgcaca gctatttata atagaattta ctttttacta gtgattttaa
    12481 ctaaatcttg aaatttactc ataatgtaat tgtaattatc ttttttatga ggtaatatac
    12541 aactactgca atctttgata cctttatctg tatacctaaa gtttccacca cagttttctc
    12601 ctaaagcata caaaggacaa tagcaaaata gacagttaaa ctcttctggt ttattagttt
    12661 tatgacaagg gaaaaattcg caatctttgt gattaaaaaa cttataattt tcgcccatat
    12721 tttcctccta attaaataaa atttaaattt tttatactat ataagatttt ggtgataata
    12781 taattatact aaataatttt atgaatagtc gataatatag tattattaat catagtatta
    12841 atggtataat tagtcaacag aaaaataatg taaaaataat gtaagtgtat tttgtaatta
    12901 gaatatgttt tataacaaaa aatgaggtga ttaagtgaaa gaaattcaag aaaaagcact
    12961 tcttgtaggt ctgaatttga ctacaatagt gaaaaaaaat gatgatatag acactaatga
    13021 atctatggaa gaattaaaag aactaacaaa agctgctggg gcagaagttg taggtagctt
    13081 aattcaaaat aaacactctg tagatgcagc ttattatata ggaaaaggta aagtagaaga
    13141 aataagagcc tatagtgact ctttagatgc tactttagta atttttaatg atgaattatc
    13201 tggtgctcaa atcagaaata tagagaatgt tgttggaaga aaagtaatag atagaactac
    13261 tttaatttta gatatatttg ctcaaagggc acttagcaaa gaaggtaaat tacaagttga
    13321 attagcacaa ttaaaatata gattgccacg actttatgga atgggtggtg agatgagtag
    13381 aacaggtgca gggattggta ctagaggtcc tggagaacaa aaactagaaa tagataagag
    13441 acatatactt aataaagcag cagatataag acgtgaactt aaagaagtaa aaaagaatag
    13501 agagacacag agagttaagc gtttaaagtc aaatataccg attgtagcac ttgttgggta
    13561 tacaaatgca ggaaaatcta ccctgttgaa cgaattaata aagacacaca aggattatga
    13621 acaagaaaaa gaagtttttg taaaagatat gctttttgca actttagatg taactttgag
    13681 aaaggctctt ttgccgaata aaaaagaatt tttagtagta gatactgttg gatttgtaag
    13741 taaacttccc catgatttag tagaagcgtt taaggcaaca ttggaagaag tacagtatgc
    13801 aaatttaatc ttacatgtaa ttgatgcaac aaatactagt tatgaattgc aaaaaagtac
    13861 gactgaaggt gtattaaaag aacttggtgt aaatgataaa aaacatatac ttgtatataa
    13921 taaagtggac aagctagaat tggatatata ccctaaaagt caagaagata ttgtgtatat
    13981 ttctgcaaaa caaggcataa atatggataa gttattaaat atgatagaaa ttgctttaat
    14041 ggaaaatact tattcagtaa gtttgatgtt accttatgag agaggtgata tatttagtag
    14101 aataaaagat aaatataatg tggagaattt tgagtatggg gaaagtggta taacgctaga
    14161 tgcagattta gatgaagaag attttaatat ctatagagag tacatactag aaaaataggt
    14221 tgaatatgga tataaactgg gaaaatatag ataatcttga agattatttt ataacctatc
    14281 ttctttataa agaatctaaa acagtatctc aaattagtaa aattagaaat atttcaagta
    14341 ctgaagcaag tgagcaactt atacaagcaa agttaaaaat aaaggaaatg caaaaagatg
    14401 attttgaggc atctaaggat attttggata aatttctaga actagataaa attaagagac
    14461 ttgattttat ggatagctta gatgacgaaa aaatggtata ctttaagaga aaggttttca
    14521 aaagaatcct tgtagaaaaa aatgctgaag atttaattgt tttaatttgg gcgacaggtg
    14581 agctaaaaga tgataggttt ttgaaattgc ttcatcagct cacaaaccac agacactcag
    14641 atattagaag aataacttat tcagcaatca gaaagattga atcaccaagt agtagagaag
    14701 tactgcaaaa aggtctctac gacaaaaatg cacagactag gcagtactgt gcaaaagctc
    14761 tttcaaaatt gggagatgaa aacagtttaa agattttaca gcagttgaaa gacaagaata
    14821 agaattttga aaaagaatat gtgcttagag cttatgacga agccattaag tcattggaaa
    14881 atgcaaaaaa tttgaaaaag taatttttta ttaaagcaat ttgtttactt aatgatgtat
    14941 ttctaaagtt taacaaggta ataaataaat taatgggagg ggtttcaaat gatagtaaga
    15001 cgttgtgcag gtggagtagt gttttatgct aataaagtac ttattgtaaa aaatgatagg
    15061 ggtgaatgga cacttccaaa aggaaagata ctaggagggg gacttccata tgagagtgct
    15121 gttcaaagag ttaaagtaga aactggcata gatgctaaaa tgatagatgt ggctggcgat
    15181 actatgtatg aatttttctc aagaagtaga caacaagaag tttgtaatgc aataatgtgg
    15241 tatgttatgg aagcttgtaa cactgattgt gtacttgctc ctgaatttca agaaggtgga
    15301 ttttataaag taaaggatgc tttagaaatg ttatcacatc ataaagagca agctttagta
    15361 gaagtatctt ataaaaagtt taaggaatta aagaaattag tttcagaggg cgatgatgtt
    15421 ccagcaaatt aattgtgaat ttagaggatt ctgaggaagg gatagcccct tcctttttta
    15481 attgaagcag ttttaagcgt aaattatata gtctctttaa actatgattt atcaatttag
    15541 agatttagta gtttatattt aaaatcagta agatatattt aaaataaaaa ttaaaaagta
    15601 tacaatttta ttatataatt gtcttatgat gttgattaaa gaagtattaa aagtccattt
    15661 atttttaaat agaatgattt tataaatata tatttgatgt gtatattaga aaatgattaa
    15721 aataggagga aatacaatga gcaattacag gacattacat gaatttggaa cggatgaaat
    15781 aacgattgaa aagtctgttt ttattggata tgctaagcct ataaagagtg aagaagaagc
    15841 attagagttt ataaatgaaa taaagaaaaa gcataaagat gcaactcata atgtgtgggc
    15901 ttatactgta ggtaaaaata tgaatataca aagatatagt gatgatggag aaccacaagg
    15961 aacagctggg atacctacat tagaggttat aaaaaaagaa gatttgagag atgtagcggt
    16021 tgttgttact aggtattttg gaggaacaaa attaggtgca ggtggtctag ttagagctta
    16081 tacaaaaggt gctaaactgg gacttgaagc aggtaagata atttataagg taatgtatca
    16141 agaagttaag gttaagatag attatactca acttggaaaa gtgcaaaatg agttaatgaa
    16201 tttggggtat tttataaaag atactgtata tgaggataat gttgaaatag tagtatattc
    16261 aagattagaa gatgtagaga aattatcaga aaaaatgatt gatataacaa gtggaacagg
    16321 aaaaatagtt ttaggagagg aattttattt gtctgaacaa aatggagaaa ttttaatata
    16381 atctaaaagg ttattagaat agttaatacc tcaaatagcc aattagtatt tttaagtgaa
    16441 aagccattaa attgatttaa tctaaatcaa tttaatggct ttttatcgga ttttgcacag
    16501 aatccttttt ttacttcaaa taaaccgact aaaagtaaaa atatcccaaa tatttttttt
    16561 aatatatctg aagacagata attagctaat agtgaaccaa atacagctcc taaaacccca
    16621 aacacagcta taggtataac caatttaaaa attacatttt tattttttat atgtatagca
    16681 agtgctataa gtgtcatagg gatagatgaa agtaaattta cgctttgtgc tattttagtg
    16741 tctatgccag caagtaaaac gagtgcaggt ataaggattg ttcctccacc cattcccatt
    16801 cctccaataa ttcctgcaaa aaatccaatt agagcaaata acattaaaac accatcctta
    16861 atgctgcaat tatcataatt attccaaaag aaattctaag aaactttcca gtaaccttat
    16921 ttagtaattt agctcctata ataccaccaa gaatacttcc aactgcaact ttaattgtta
    16981 agttaacatc ataagtacct ttagatacat ataaaacaga acttgctgtc gttaagaata
    17041 ttattataga aagagcagtt gcatgagatt tatgttcttc taccttaacg atgtcattta
    17101 atattggaac taaaagagta cctcctccag aaccaaaaac tccatttata aatcctgtaa
    17161 aaactccaat tatcgtattt tttaaattaa aatcaaagta ttttttcttt aaatcatcag
    17221 tttttttcat aaaaatcacc ttctgaaata gttttaccaa aataggcaaa ttatatttat
    17281 ataaaataag attaaatata atctttcatg tttgtttaat tttgcataaa aaaaaacata
    17341 ttaaaacaac atattaatat atggtataat atattaaaac aaagtagcaa atttgagagg
    17401 agataacaat gtcaaatata aaaatacaaa tagacaaaac tgtagaatgg cttataaata
    17461 aggttaatga ggcaaatgca aaaggattaa ttgttggtgt atctggagga attgattcgg
    17521 cagtagttgc taatttaatt aaaaaggcat ttccagaaaa ctctatggga gttataatga
    17581 gcataaaaag taatcctcaa gatagagaag atgcacttaa ggttatagag ggatgtgata
    17641 tagaatacct tgatttagac cttatagaac cacaaagtgc tatattagac atggtagtag
    17701 gaaatttaaa agataaacat ttatatagag aagaatattt aaagatgaca gatgcaaact
    17761 taagagcaag agtaagaatg agtacaattt atacaattgc taacaatcta ggatatctgg
    17821 ttgttggtac agacaatgct gcagaaatac acacaggata ttttactaaa tttggtgatg
    17881 gtggagttga tattttacct attgcaaatt taactaaagg agaggtgtat gaatgggcaa
    17941 aagagcttgg tgttcatgaa gatttaataa acaaagctcc ttcagcaggt ctttgggaag
    18001 gtcaaacaga tgaagatgaa atgggaacta cttacaatat gatagatgct gtattagaag
    18061 gtagattgga tgaagttcct aaaagagacc aagagataat tgaaagactt catagattaa
    18121 gtgaacacaa gagaaaaacg cctgcacaac ctcctaagtt ctaatttact atttataaga
    18181 tttgtgataa aatttacaag tgatgaaaat tagaataatt aattgtacgt attaaggagt
    18241 ggaaatatgg gacgtatagg taatataatt aatagaaaag gtaaacaaga tgctcaaaga
    18301 gcaaagatat ttactaaaca tgctagagca atagcagttg cagctaagga aggtggagca
    18361 gacccagagt acaatgcagc gttaaaaaca gctatagaaa aagctaaggc agataatatg
    18421 ccaaatgata atatagatag agctatcgct aaaggtgctg gagcaggtgc tggagaagac
    18481 tatgaaacta tagtttatga aggatatgga cctggtggag ttgctgttat agtagaaact
    18541 ttaactgaca acaaaaacag aactgctggt aacgtaagat actattttga caaaaacgga
    18601 ggaaacttag gaactagtgg atgtgtgtct ttcatgtttg ataaaaaagg acaaatatta
    18661 gttggtttag gagatggagt ttcagaagaa gaattgatgg atgttgcatt agaagcagga
    18721 gctgaagatt tcataacaga agaagatggt tatgaaataa taactacacc agaagatttt
    18781 tctagtgttc gtgatgagtt aaaagctaaa ggatatgagt ttatatctgc tgatgtgaag
    18841 atgatacctc aaactacaac tgtattgact gaagaatctc atttaaaaat gatgaataaa
    18901 ttagttgata tgcttgaaga agatgatgat gttcaagata tatatcataa ctgggaagta
    18961 gaataaatag tataagcata ttgaaattta taagtattga atcaatttta acatgtgata
    19021 aattttatag aataagaaac actaaaatag acatacgcac ctctaaccta tatttgcata
    19081 ataatatgta agcataggta aggggtgttt ttatatttat aggttaagaa atttaaagta
    19141 aattttgagc tggactcgat tgtctataaa taattgatgg gtatataact tgttgctgaa
    19201 taccatttat acgcccagtt actgtatcaa taatcatcct aagactacgt tctgctgtct
    19261 cttccatttt taaatctata actgtcaaag gaggaatact tcttgccatt agtcgtgaag
    19321 gtccattact tgctgcaaat atttgaatat cctctccaat tacaattccc gattcatgaa
    19381 atgcattcat cataccaaga gctaccacat catgattaca aaaaattact ttttttagtg
    19441 aattttttgc aagcattttt tgagcaatat catatccact atctatttta ttagaacaat
    19501 aatatataga atctgataga tgtattccat gttctttaca tgtttttaaa aaagatttac
    19561 cccttaaagt catacatgta aaggaagttg ggttgaatac aacagcaata tcatttttac
    19621 cattttgaat agcatgtaaa gctgctatac gaccagtttc ctcattatca atagatacac
    19681 ttgaaaaacc aggtatttca cgatttacaa caactacagg aattttaggt ttatttttta
    19741 ctagatattc tatatcatca gcattgatac ctgttaaaac aattccatca taataattat
    19801 ttgcaaaaaa gtattcatca tcacatagat gaccaacttt gtaaggtcgt atgatgatat
    19861 ttatgtttaa atgttctaaa gaaattatgt ggtttattgc ttgaagtata ttggttgatg
    19921 ccaactcaaa tccctcatcc tgccaataaa aagctatttt aatattatta tgtttaatag
    19981 gagtagagcg tttatatcca agcttatcag ctgaatttaa tataagctct tgtgtatgta
    20041 gcggaatcct gcgttcattt cctttaccac ttaagacgta cgagacagtt gctgaagaaa
    20101 gaccacagtc ttttgcaata tctttgattg ttgccatgat aaaatcctcc ttaaatagtt
    20161 gcttaaattt gcttatacca ataatcttaa catatattca actaaagaac aatattctac
    20221 aaggtgcata ttttacagga ttagaataaa taaattaaaa aaaacttctt acttatgttg
    20281 acataattta gtttatagga ttataatatg cttaaataag cttatgatta acttgaatta
    20341 agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgcaaa gggggaaaag
    20401 gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa aacctgaaag
    20461 agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct tctgggatta
    20521 aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg aaagatgcaa
    20581 aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat gcattagttc
    20641 caaatttata tggtgctaaa gttgcatggg agtgtggtat aagagatata tcctatgtta
    20701 tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa gagtctattg
    20761 atgagcttaa aaaaataatg aatacttatc cagacatgaa tatatgtatt ggaatggcta
    20821 ctgtatttgg atgtcctttt gaaggtttac caaaaattga agatgccata agatttgcta
    20881 aacgcctttg ggaaaacggt atcagggaga taaatctagc agatacaata gggattgctg
    20941 accctaagca agtaagagaa actattagtg cattagttga agctttacca gaatgcagat
    21001 ggcaagtaca tattcatgat actagaaata tgggaatggt taatactctt gtggctattg
    21061 aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc ccatttgctc
    21121 ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa cgtatggggt
    21181 atgatacagg agttgatttt gttaaacttc ttgaattatc aaaatatcag aagagttttg
    21241 taaatggagt atatagtggt catcatgtaa atataaagtc tgatagttgt aattgttagt
    21301 tctattgatt aatcgattaa aaaaatatgc tgattggtaa aatagattta ttgataaaaa
    21361 atataaatta taaaatacat tatctaataa aacaaataaa aaaattgagg gggattagta
    21421 tggatattat agctttactt ttatttttag gagttattat tattgcattt gtacgtaaga
    21481 ataatgcagg tattttggct ctagctattg gtgtcatagc tgttagaatt tttggaatgg
    21541 atgataaaga actttatgga gctataagca actcaatgtt tactacactt gttggtataa
    21601 cactactctt tgcaatagtg aactcaacag gagcattaaa gcttgttgca gaaaaaattg
    21661 tagctgcttc tggtaaacgt gtttggctta tacctatagc agtttttata gcaggatttg
    21721 ttgttgctgg tgttggacct ggggcaattc cagcacttgc aattatccca gcaatggctg
    21781 ttcctgtagc cataacagtt ggttataatc ctgtaatgct tgcacttata ggtgaatgtg
    21841 gattaatggc aggtcgtatg actcctatta ctccagaagg tcaaattata acaaccgttg
    21901 cagaaggggt tggaattgaa aatgttatgc ctactatctt agcctgtcaa actctttcta
    21961 caattgtatt ttcaatagta ttgtttataa ttttcaaagg atataaactt aaaaaaccta
    22021 taaatgttat agatagtaag caaatagaaa aattcaatat aaagcagata atttctctaa
    22081 taagcatagt agttatgatt gcttttatca taatttttga tatgaatgtt gggcttgcag
    22141 cttttgcagt ttcagctgta cttatttttt tcaatattgc tgatgaagga gaatgtatca
    22201 aatcaattcc atggagcacg atagtaatgg ttcttggagt aggtgcaatg atgacaatcg
    22261 tagatgcagc aggaggtatt gacttgatga gtaatgcatt atcatcactg atgaatagta
    22321 aaacagctac tccaattatg agtatttcag caggacttat gtctatggtt agttctgctt
    22381 tagcagtagt ttatccaact atgatgccta tgtgtgttga catagctaag gcagttggag
    22441 gagtagaccc actagcactt attgctgcag ttggtgttgg aggttctctt gctggtgtaa
    22501 gcccactttc tactggtggg gcattgattt tagcagctat gggttcaagt aagaaagact
    22561 ttaataagga agaacaaaat aaggtttttg tacagctttt cattatgagt gcagttggct
    22621 tattggttat tgcagtagta tcaatattat catttaatgc aataacacat ttaatgaact
    22681 aattagaata actgaaaata aatcattaaa tttaactttt aatattaatc aatataatat
    22741 aatttggagg ggaagttatg aaaaaaggag cattaagcaa tataaaggta cttgacctta
    22801 caagagttct tgcaggtcca tattgtacaa tgatgttggc ggattttgga gctgatgtta
    22861 taaaaataga gatgccaaga aaaggtgatg atacaagagg ttttggacct tttgtaaatg
    22921 gagagagttt atactatgca aatataaata gaaataaaaa aggtatcact ctaaacctta
    22981 aatcagaaga aggaaaaaat atatttttag aaatggtaaa acatgctgat gttgtagtag
    23041 aaaattacag accaggtgta atggaaaagt taggtctggg ttatgatgag ttggctaaag
    23101 ttaacaatca aataatttat ggagctgtat caggatttgg atgttatgga ccatattcag
    23161 aacgtccagg ttatgatatt attgcacagg caatgggtgg tcttatgagt ataacagggc
    23221 aagaaggagg agaaccaacg agagttggta atgctatggg tgatgtacta ggtggaatga
    23281 atttaactat tggtatactc acagcactca acgctagaaa aataatagga aaaggacaaa
    23341 gagtagatgt ttccctcgtt gattctgttg tatcgtctct cgaaactggt atacagagat
    23401 actttgattc aggacaacct gaacttatgg gtaatagata tgcttcagcc tatccttatg
    23461 actcttttaa ggcaagtgat ggaagttttg taataggctg tggtaatgac aaactttttg
    23521 aaaagttatg taaaaaagtt ctttttagag aagacctttt aatagatgag agatttaaaa
    23581 caaatcttaa tcgttgtgaa cattacaagg ctcttaaacc agaaatagaa aaatggtctg
    23641 ttaagcatac aataaaagaa gcagttgact taatatcaga ggctggagtt cctgctgctc
    23701 ctatctataa tcttgattgt gttacaacag atgaacatat agcaaaagca agagaaatga
    23761 ttgtagatat tagtcatcct attataggaa atatgaaagt caatggaaat ccagttaagt
    23821 tgatgacaac aaaagctaaa attgatatgc cagcaccatc tctaggggaa agtaacgagg
    23881 agatttataa tggttggctt ggttttgata gtgataaaat aaaagattta aaagaaaatg
    23941 gagttatttg attaaaaaat agctaaacac acttgtgact ttagtcatgg gaggttcagt
    24001 tagaataata tcaaaaaata ttaaatcaat actgtaaata aaatatagaa ttgtcagaag
    24061 catttaagat gggaaaagct acttagacat tgagtctttg tggcttttcc tattttcatt
    24121 aagaatttta tattatcaga atagatatgt actaaactaa agtattaaca atttaaatta
    24181 atagaaaata aactacctaa ttatgttgaa taaagactac tttagtgaaa ataaacttat
    24241 agagatgcta tttaaattat tttaatggaa atatcaaaaa tgagactgca ttctcaaaaa
    24301 tgagactgcg ttttttaagt attttctcga aaatgagaca aaaatattgt tttatgggtt
    24361 ttattgaaga aaaattcttc tgtgaattac aatttaatca attttaaagt tgaaaattat
    24421 taaaaattta gtgaaattta tgtatatttc gtttatttta ttaaaaataa caatttttta
    24481 ctaaaaataa agaacttcaa tggtgattgg catagtactt gctattttat attaacaaga
    24541 aatgtagaaa aaataacaat gtataatggg gggaatatga tggaatctgt aatggaaaat
    24601 ttgaacaatc ttaaggtgga ggttaaggac aaagtctgtg ttattactat aaatagacct
    24661 aaagctttaa atgctcttaa tagtgacact cttagggaac tatcacaagt tattgatgta
    24721 gtatcagaaa atgaggcaat attaggagtt attattactg gagaaggtaa agtttttgta
    24781 gccggtgctg atatacgcca gatgcaaaac tataaaagtg aggaaggcag aaaatatgca
    24841 ggttatgctc agggaatttt tgataaaatt gaagctcttg agaaaacagt aatagcagca
    24901 gttaatggat atgcattagg tggtggatgt gaacttgcta tgagttgtga tattagaatt
    24961 gcatcagaaa aggctatatt tggacaacct gaagttaatt taggagttat tccatgtttt
    25021 ggtggaactc aaagattatc aagacttgtt ggaactggaa tagctaaaga gttaattttt
    25081 acggggagac aagtaaatgc agaagaagct aaatctattg gacttattaa taaagtggtt
    25141 ccaagtgatt tacttttaga agagtctatg aagatgatga accaaatagt tgaaaaagcg
    25201 ccaatagcaa taagatatgc aaaagttgtt attaacaaag ggatagatat ggatttaaag
    25261 aatgctcttg aactagagaa agatgttgca ggtttaactt ttgctacaag agataagcaa
    25321 gaaggaatga atgcatttat agaaaagaga aagccagttt ttgaaaataa atagctatta
    25381 taactaaaat tggaacaatg tagaatatat tcaaaatcta aatttcgtga aaaaatacat
    25441 atagtttaat tttaaaagga gttaatttat gaatacggga gtaattataa gtttagtagg
    25501 gattatcata gctctatctc ttctagttgt aatggttatg aagggaataa atatttttat
    25561 tatagcaatt gtatgttcat cattagttgc tataacagga agcctaaatg tgtatgatgc
    25621 acttaaagtg gattatatga cagggtttgt tggatttctt caggcaaatt tctttatttt
    25681 cctaacaggg acattaatgg gaaaaataat ggagattact ggtggagcaa aatctattgc
    25741 taagatgatt gtaagatgga ttgggaagga taaagcacta ctttctattc caatagcttg
    25801 tggaatatta gcctatggtg gagtaagtgt tttcgttgta agttttgcag tgtttccgat
    25861 agctttagaa atttttaaag aagctgattt gccacgaaga tttataccag cagctttgac
    25921 ttttggatgc tcaacttttg ctatggttgc accaggggca ccacaaattc agaatattgt
    25981 tccagcatca acattaggga cagatattat ggctggagca gtaaatggtt ttatatcatg
    26041 tgcagtaatg tttatagttg gaagtacaat tttgtacaaa atggtttcaa aagaaaaagc
    26101 caatggtggt cattttatag cacatgaatc agatatattt gatgaagcag aagcaactaa
    26161 ttctaagaga gaaaatggtc ccaatggtgt aattgcctta attccattga tagtgtcgat
    26221 tttaataatt aatgtaaaag ttaatggaaa agcgatagta ccaattgagg tgggagtatt
    26281 tattggagct ttattagtat atttattgtt aaataaatat caagataata ctaaaatagt
    26341 tggacatgtt ggtgatgcat gtaaaactac agtagtagca atatgtaata cttgtgctgt
    26401 agttgcattt ggttcagtag taaaatcagc agttggtttt gacttcatag ttaatgctat
    26461 gactaatatt cctggtcctc ctattgcagg ggctgcagtt ggaactacag ttattgcagg
    26521 tatttgtggg tcagcttcag gtggactagg aattgcagct ccacttcttg gaccagtatt
    26581 tttagccaag ggtgtttccg ctgcagcatt aactaggact atggcaattt catcagcagc
    26641 attagattca cttccacaca atggatatat tgttactgta acaaatggtc tttgtaaaga
    26701 aacacataag gatgcctata tgccaatatt ctgggtaact gttttaaccc catttattgg
    26761 aaccatagta gcagtcctac tgtttacaat gttcccggga ttgccatagg agaaaagata
    26821 aaaggtataa attatatttt gatattataa aatcaatttt caaaatatat acaataaatt
    26881 tcatgtttta atactgtaaa tatgtattta aaataacaaa ttgaccatat aaggaggaat
    26941 tgaaagtgtt aaataaacca ttagagggag ttaaggttat tgacctaacg tattttgtag
    27001 caggtccagg gacttctaaa atattagctg actggggtgc tgacgttatt aaggtagagc
    27061 caagctttgg agacccagga agaaagacag gggcaacaat gacaatgcca atagatgatt
    27121 ataataatcc tttttacagc acatataatt caaataaaag aggtttgtct ataaatctta
    27181 agagtgaaac tggaattgaa attatggata agcttttaag cgaagcaaac gtatttgttt
    27241 caagttatag aacaggagca ttaaaaagac taggtcttga ttatgaatca ttaagtaaaa
    27301 agcatccaca ccttatctgg ggtcagataa atggttttgg cgattttgga cctgctaagg
    27361 ataatgcagg atttgataca gttgcctttt gggcaagaag tggggctatg cttgatatag
    27421 cagaaaaaga tacaagtcca ataaatccac ctatagcctt tggagatgca acaacttctt
    27481 gtagtttatc tggaggaata tgtgcagctt tgtaccagca agctaagact ggaaaaggac
    27541 aaaaagtaat ggcatcttta tttgggcaag caatatggaa tgcatcatct ttattagcat
    27601 ctacacagtt tagtgatgag tatccaaaaa ctagaaaaaa tgctatttca ccagtaataa
    27661 attcttatcg ttgtaaggat ggaaagtgga tattcttaag tattttagag catgaaagat
    27721 attttgaggc actttgtaga ttatttggaa gagaagactt aataaaagaa gagaaatttg
    27781 catcttcagt agaaagtaag aaaaatgcta cagaacttat taatatttta gatggtgaat
    27841 ttgcaaagtt tactcaaaat gagatggttg aaaaacttac tgctgctgat atagctcatg
    27901 aaaaaattca acatgttaat gatgtagtga ctgatgaaca ggctattgct aataactata
    27961 tatatgaaca tacaagtaaa gatggaaata agacaatgct tgcatctact ccagttaaat
    28021 ttggaaatat tgaagttaac atgacttgtg atgctccact tataggagaa cattcagatg
    28081 agatattgag agaacttgga tacagtgatt ctgatataga gactctaatt gaatctggaa
    28141 ttgtaacaat gaataaagaa gcatgtgaaa tatgttaata aacatgtgaa gtatgttaat
    28201 aaataggagg gaattttatg gattttagat taacagaagc acaattaatg cttcaaagag
    28261 tagcaaaaga atttgcagag aatgaaatag caccaatagc tgctgaaaca gataaaacag
    28321 gtatttttcc aagggagctt tttagcaaaa tggctaaaat aggatttaat ggaattggaa
    28381 cacctgtaga gtatagtggt tctggtgggg cagatataga aaaggtaatt gttgttactg
    28441 agattgccaa aaaatgtgca gcatctgcag caatattatc aatacacaca atatatgctc
    28501 aagctatttt gaaatttggt actgaggaac aaaagaaaaa ataccttcca atgatggctg
    28561 aaggtgggtg tgtaggtgct tttgccttaa ccgaacctaa tgctggttca gatgctgcga
    28621 gagctgcaac aactgcaatt attgatgaag aaactgatga atatgtttta aatggaacta
    28681 agtgttttat ttctggtgga ggtcaagcag aatcattaat aatatttgca ttgacagacc
    28741 catcaaaggg aataaaggga atgtctgcta tcattgtcga caaaggtact ccaggttttt
    28801 caataggaaa aattgaagaa aaaatgggta ttcatggttc agaaactgca gagttaatat
    28861 ttgataactg tagagttcca aagtctaact tattaggaaa agaaggtaaa ggatttaata
    28921 tagctatgac ttgtttagat ggagcaagaa ttggagttgg agctcaagct gtaggaattg
    28981 ctgaaggggc tttagaagaa agtatcaaat attctaaaga aagagtccag tttggcaagc
    29041 caatttcagc tttgcaaggt attcaatggt atatagcaga tatggcgaca atggtggaat
    29101 cagcaaaatt attagtatat tatgctgcag atttgaaagc tagaggagaa aaacacacta
    29161 aagaagctgc tatggcaaaa tacaatgcgt cacgtactgc aagagaggtt acaaatttag
    29221 cccttcaaat ccatggtgga tatggatata tgaaggatta tccattagag agaatgtaca
    29281 gagatgccaa aataacagaa atttacgaag gtacttcaga aatacacaaa gttgttattt
    29341 caagagcagt actaggatag gaggaaaaga atgaatatag ttgtttgctt aaagcaagtt
    29401 ccagatacta atgaagttaa aataaataaa gagacaggta ctctaataag agatggtgta
    29461 ccaagtataa tcaatccaga tgatagaaat gctttagaag aagctctaaa aatgaaagat
    29521 gaactaggag cagtgattaa ggttataagc atgggacctc ctcaagctaa aagtgcttta
    29581 aaagaagctt tggcaatggg agctgatgaa gcttatttaa tctcagatag agcttttggt
    29641 ggttcagata cttgggctac atctacaatt attgctgctg ctatagagaa ggttggaaag
    29701 tatgatgtaa tattctgcgg aagacaagct atagatggag atacagctca ggttggacca
    29761 gaggttgcag aatttttagg gataccacaa gttacttatg cgaaagaagt aaaggttcaa
    29821 gatgacaaat tactagtaac tcgttataca gagacagggg attatttaat agaagcaaag
    29881 atgccagttt tattaactgc tataaaagag ttaaacaatc caagatatcc aagtgtcaag
    29941 ggaatcttag aagcttataa taatggagat gcaaagatta ctgtattaac acttgcagat
    30001 ttagatgttg atacaactca aattggatta aagggttcac ctacaaacgt ttataagtct
    30061 tttgtaccag ttaaagacaa gcataatgaa ataatagaag gtataaataa aaaagaaaaa
    30121 gctgagaaac taatagaaat actattcgat ttgaaactag tataggagtg ttaagatgat
    30181 gagagcaaaa gttaatcaag gtataaattt aaatgattat aatggtgttt gggtaatagg
    30241 agagcaaagg gaaggtaaaa ttaatcctgt tacgatagaa cttattggtg aaggaagaaa
    30301 gcttgctgac caattaggaa aagagcttgc agttgtgata gctggatatg aagtagagaa
    30361 agaagtgaag gaattacttc actatagtgt tgataaagta tattacatca atgaccctct
    30421 tcttaaagat ttcactactg atggatatgc aatttcaatt gcaaatttaa tcgaaagaaa
    30481 aaagccagag gttgtattgg ttggtgctac ttctatagga agagatatag cgcctagaat
    30541 tgcaggtaag gttggaacag gtctaactgc tgactgtaca aaacttgaaa tagattcaac
    30601 agataataag ttattacaaa caagaccagc atttggtgga aatttaatgg ccactattgt
    30661 ttgtccaaaa aatagaccac agatgtcaac agttcgtcca ggagttatgg caaaagctgt
    30721 aagaaacgaa tcagagactg gaattttaga agttgttact ccagaactta ctgaaaaaat
    30781 gattagaact agattagtag agatacttcc acaagaaaag aaatctgtaa atctaactga
    30841 tgctagaata atagtatcag ggggaagagg attaaaaaga gcagaaggat ttgagcttat
    30901 aaaagaattg gctgataaat tgggggcaga gattggtgct tcaagagcag cagtagattc
    30961 agggtggata gaacattctc atcaagtggg acaaacagga actactgtta gaccagaact
    31021 atacattgca tgtggaatat ctggagcaat acaacattta gcaggtatga gtgattctaa
    31081 atatatagtt gctataaata aagatgcaaa agcccctatt tttagtatat gtgactatgg
    31141 aatagtagga gatttatatg aaattatacc tgaaatgata gaatcattaa ataggtaact
    31201 tttagaaaac attgaatatg tagaatgata agtcttataa tattgtattg taagacttat
    31261 tatttttgct gaaggttata gtggggagat gaaacaatga aagagcagtg gtataaggat
    31321 atatttgcaa gagtattatc tatgactgat gatggtttta ttgttgtaaa tacaagtgga
    31381 gtaataatag atattaatga taaatattgt gactttttag gaaaagaaag aaaagatata
    31441 ataggacaaa atatacaaag tattatacca aatacaaaaa tgctagatgt tatgaaaaat
    31501 aagtattgtg aagaaggagc tatacatcat tattcaggtg gaaatacaaa agaaaaaagt
    31561 gtaatagtaa gtaggtctta tgtggaaaat gacaatggag aagtagttgc aggagtagct
    31621 caagttaaat ttagattgca atcatttgat gtagctaaaa agttaatgtc agaatatatg
    31681 gagctccaat attataaaga gcagtttaag gataactgtg gttttgacaa gctcataggt
    31741 gaaaatagag acttcataga acttaagaaa acaggtgtga aagcttctaa aacaaacttt
    31801 ccagtacttt taacgggtga aacaggcaca ggtaaagaag tttttgctag agcaattcac
    31861 aataatagca gtaggtcaga taaacctatg gttagtataa attgcgctgc aataccagaa
    31921 gaattattag agtcagagct atttggctat gacgaaggag cttttacagg agcaaaaaag
    31981 ggtggtaaaa aaggtaaatt tttagttgcg aataatggta caatcttttt agacgagata
    32041 ggtgacatgc ctttgaccat gcaagcaaaa cttttaagag ttcttcagga aagtgagata
    32101 gaaccagtgg gaggtcttaa aactataaag atagatgtaa gagtaatctc tgccacaaga
    32161 aagaatttaa gtaaaatggt tgaagaggga ttatttagag aagacctata ctatagatta
    32221 aatgtaataa acattcatat gatggaattg aaggacaggc aagatgatat tttattgcta
    32281 gcaaattata tattaaacaa attaaatgtt gaatacaagg aattgaaggt tttaagtgat
    32341 aaggttaaaa attgttttat taactatact tggccgggaa atataagaga actacaaaat
    32401 gtaataaaaa gtgcctatgc tgtaagtgat gatatggtca ttatgatgtg tgaccttcca
    32461 tctaagatgg ataatatcag cagagtagct caatgtaatg tggatagcaa ttgttcaatt
    32521 catgaaatgg ttgagaatta tgaaaagagc ttaattattg atgtgttaag gaagtataat
    32581 tggaaatgtt ctaaggcagc agaggttatg ggaatacaca agagtctttt atataagaaa
    32641 ataaaaaagt atgaaattga attaaataat taagataact tgaattaagg caatgctaaa
    32701 aaagcgccct tattcaaagc gaactgtata aactgtttta tgcagttaaa acgtgtaaaa
    32761 gtagatgtga agtactaatg tcaatcatta gtactttttt attatattta taattactat
    32821 ctatatttca gtatcttttg atatctaaga ttattatgtt taatttagta tataacaaaa
    32881 ccctcttagt tatggacagt tttttatatt ttaactgtct actaagaggg gatagtttag
    32941 tttaaattta gagtaaattt tatctagcat ttataaaaaa tattttgaat agcttttttg
    33001 ttatttagtc ataattttct acttctttat caagttcttt gccacaagaa tcacacttat
    33061 atatagcatt gtatccgtaa tcatattcat caactatttt cttttgtcta aatacaccac
    33121 cacactcaca taggaaacca atttttataa gtgtaacgat aaattgtgat tttataaatt
    33181 ttatatcatt gcatttatcg ttagtgtaaa atgtataaat ataagagtta tcgcactcat
    33241 ctcttaattc aaatctttca atatatgtgt tattttttaa ttcctcaaat tcttctttag
    33301 ttaaatatga tttttgttgc aattcatgta aacgcttaat ttggtccatt atttatatcc
    33361 tcccaataat tttagtattt taagcttaga taaggaatct aagttttatt aaatttatcc
    33421 caaggaatta tttaaatcat atttagtttt attaattaca acatatataa attacacgaa
    33481 aataaaacta attgtaaaaa taatatcctg tgaaaaccta aactactaac cccttactaa
    33541 cccgaaaact aaaaaaagaa aatatatcca aatataagtg atatataaat aaagatgttt
    33601 catataaatt taactttaaa tattctgaca ataatatata taaatatatt atagttaaaa
    33661 tataccacat attttgggta ttgtatactg gaattagagt aattaattta ttttttaaca
    33721 tacaaatgat tttatgaaaa aaaacaagac tcaaaaattt ttatattctt tgatagaata
    33781 aaattgagag taaatttatt gaattttata aaatttataa ggggtgagtc taagatatgc
    33841 atgaagataa aaatgatata tttgaatcaa tagatactga ggattttgat aataaaacag
    33901 aagaagaatg tttagagcta gtaagaaaaa atggattgaa tttaatatac ataaaaaatc
    33961 aaacagaaga gatatgtcta gaggctgtta gacaaaattg taatgcaata aaatgtgttc
    34021 agaatcagac agaaaaaata tgtatagaag cagttaaaca agattggaga atgttagaat
    34081 ttgtggaaga gccaacagaa gaaatttgca tgttggctat taaccaagat ggaacagcat
    34141 taaaatatat agaaaatcag acagaggagt tatgcttaag agccgttgaa aaaaatggtg
    34201 cagctttgga gtatgtaaaa gaacaaacag aagaaatatg tatagaagca gttagaaaca
    34261 gcgaatttgg attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg gaagcagtta
    34321 agcattgtag ctataatcta aaatgggtag aaaatcaaac agaagaaata tgtatggaag
    34381 cagtgagaca aaatgggctt gatttgaaat ttgtaaaaaa tcaaacagaa acaatctgtt
    34441 taagagcagt aagacaaaat ggaatggcat tagaatttgt aaaggaacag actgttggta
    34501 tatgcttaaa ggcagtaaga caatatggaa tggcattaaa atttgtaaag gaacaaacag
    34561 aagaaatttg tacagaagct ataaaacaag ataaaagagc acttagtttt gtaaaaggag
    34621 ataaggaaaa atataaggct ctatatgata ataatgaacc atttgcaaag agatatgtta
    34681 gaaatgtaat agaaaaagaa aacagagcat tgattaaaaa aggagaagaa aatgcaaaaa
    34741 tcaaaattgc aggaaaactt tatgatagag gcatgtcttt tgaagatata tcagatatag
    34801 ttgagattga aataagcaag ttaaaagtga gtttaggtgt tttttaaagt aataaatgta
    34861 ggtgtttttt atgggaaata gttaataaaa aaataatcga ctagctcttt gtaggacagc
    34921 aaaattagtg atataatttg atgtataata aattattttt attagataag ggggaattaa
    34981 agttgaagat tacagtatta taccaaacaa gaagtggaaa cacagaaagg gtcgctaagc
    35041 tgatagaaga gggcgctaaa aaggtggatg gagtagatgt aaagcttatg agacttgata
    35101 atattgattt agattattta aatgaaagta aagcagtaat atttggaaca cctacttatt
    35161 tagcaaatac aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa aaagttaatt
    35221 tagctggaaa attaggagct gtttttgcta catgtgatta tatatgtgga ggtccagatg
    35281 ttgcgatact tactatagta ggacatttga tggttaaagg gatgcttgtg tattctggag
    35341 gtggttcttt aggaaagcca tttatacatt taggacatgt acattcaatt gaaggtccag
    35401 agttacaaga tgagaaggct ataatatttg gtgaaagaat tgctactaag gcaaaagaac
    35461 tatttgctta atttctatat gcatagtaat gattagagta aatttagggt tttaatggag
    35521 atttgaagta aaagttataa aataaaaaat tttccataaa attactcaag atgaattata
    35581 atataattat aaataaatta tattattaaa atatttaaaa tcaaacgtca atagcgaggg
    35641 aagtttatgg tatggcatat agaatatgag attttttctg caattatagt tattttttta
    35701 atggtatact tttttagaag taaatttata cctactttac aaaacaaaat atattgtgca
    35761 cttttaatat tctcattttt atttataatt tcaaatattt taggttcatt ttgtctaaat
    35821 aatatagaca aaattccaat ttttattact tttttattga atcagatata cttattgtta
    35881 ttgccaatac cagcagcact tgtatcattt tatgttatgg ctattattta tcaagatata
    35941 aggtatatga aaaaaagatt attattttta tctataccac ttataatttg tataatctta
    36001 tctattacaa atccttttac gcatattcta tttggattgt caaaagaaac aggttatata
    36061 cgaggttgtg gatatgttgc aacttttgca tcttttttct tttatgcaat atattcagca
    36121 tttttatctt ttaaatatag aaaagcaatg catgaatcaa aaatatgggc tattagaggt
    36181 tttcttatag tttcaattgt ggcaattatc ttacaggcaa tttttttaca gtatcttctt
    36241 acaggaactg catgtgcatg tagtttactt ttgacatatc tttctatgca gaatcgaggt
    36301 cttattattg atgaccttac tggtgtactc aatagacaat cttttattca agagctagat
    36361 ttgaatatca atactgaaca atcaggattt attattacaa ttgcgcttga tgattttaag
    36421 tttatgaatg aaacttttgg aacaaaaaat ggagatattg cactaaaaga agttggaaaa
    36481 tatttgattc agatacatga tgtaaaccat gtatatagaa tagggggaga tatattttct
    36541 attgttgtca atagtcaatt aggtatagaa ccagatgata ttatcagaaa gatagaagaa
    36601 agatttaaaa agccttggct agtagaagag ataagtttta atttagcaac tagtattgct
    36661 gttgtttatt acccagaaaa tgcagaaacg acagaagatg taattgttgc tatagatttc
    36721 tctatacatg aagcaaaacg ctctgagtca aggcgagttg tttatgcaga tgcgtctata
    36781 agtgaaaaga taaagaagaa acatgttatt aaaaattgtt taaaagaagc attaaaaaat
    36841 gatgggtttg aagtgtacta ccaacctata ttttctaata gcaggggcag gtttacttca
    36901 gcagaggcat tacttaggct agaacacaaa gagttaggtt ttatacctcc tgatgaattt
    36961 attccaattg cagaacaaac aggattgatt aactcaattg gattagtagt ctttgagaag
    37021 gtatgtagat ttatagctag tgatgagttt gaagatttaa aattggataa tatagctgtg
    37081 aatctttctg tagtacaatg tatgcaaaaa aacttagctg atgatttact ttatcttatg
    37141 aataaatata atgtatcacc aactaaattt aaacttgaaa ttactgagac tgtggctatg
    37201 ggctctttta atataattaa agaaacaatg gaaagattaa ttgatttagg tgttaagttt
    37261 gcacttgatg attttggaat tgcttattct ggagttacta atatgctttc tttaccattt
    37321 tcagtaatta agttagataa gagtttgatt tggtctatga atgaagatag taggcataaa
    37381 cttacagttg agacgattat agcacttata aataaattga atatgaaggc tgtagcagaa
    37441 ggtgttgaaa ccattaagtg tgctgaagat ttaatagcta tggagtgtga atatttgcaa
    37501 gggtattatt tttctaaacc agttccagaa gatgttttta aaaatttgtt gaaagaaaat
    37561 acttttacaa gttaacaccc tttatttttt taattacaac tttgttcatt gacactgcca
    37621 gcgatatatt ataaaattaa cacaataata aatattaaat ttattataca tattattaat
    37681 attaattaat ggagtaaaga tttattatat attttattta attatcctaa agtgagaaag
    37741 gaagatgtga catgaataaa aagaaaatat tagtgccaat tgatgggaca gaaagaagta
    37801 tgcattcttt ggaatttata aaaggaatat ttaaaaaaga tgaagttgaa gtagaaataa
    37861 tgaatgtaaa agaactagta tttattgatg gcatatccct ggctgaagag ataaaaaatt
    37921 ctgaaaattt aggaagaaga atcttggata aagcagcaga gataatggga gagtatgatg
    37981 ttaaagtaca ttttactttt ggatatccag gagatgagat aattagaaaa gcaaaagaag
    38041 ataatgtaga ttttatagtt atgacaaaat ctactaaaaa aggtcttaca agaatgatag
    38101 gttcagtaac agcaagtgta gttaaacaag caaaatgtat agtgatgatt gtaccggagt
    38161 agaattagaa aggtatttga aatatgaata aaaagatagt agcagtttgt gaaagtgaaa
    38221 gaagtttaaa ttctcttaag acagctgcaa atgatttagg atttgattta gtttatgaaa
    38281 ttcaagaaaa tgataatata aaaaatgaac tttctattta tgatatagaa gatgcatcca
    38341 tagtgttatt tgttacaagt gattctatag aaagcataga gaagattgat agatttatag
    38401 atagagaatt ttatgaagtt gatccaaagt atataataga ggatgcaaaa tctatagtaa
    38461 gtgaaatttt aatagactta aattaatcgt gggtgtcgca ctaagaatag agcgacgcct
    38521 ttttatatat tttacacaag aaataaagag gttgtatcaa aatatagata agagtctttt
    38581 tcattgttga aatgatgaaa atatatttga tttcatacaa aaaagagtac ctcataacta
    38641 aaaagttcac tttgagacac tccattttta tatgttagtt gcatttaagg taaaatagaa
    38701 gtttaatcat gttgaaataa ctgaattaca tcttctaaat gattgtacat tgatgtataa
    38761 cctaagtaag agtctttact atcaaatgca atcattatct tgttatggtg gctaatcata
    38821 tccttgagag aatttctata tttaaaacca aagtattggt tagaagaaac tgttttaggt
    38881 attaactcat aatattttat aaaaattgga ttatgagaag ctaaaattat ctctttgtga
    38941 aattgcaagt cagcatcata acattcttct atcaaaccat gagaaacctt atccaattcc
    39001 tctattgact ttaggtaagt atattttatt ttttgaagtt ctttagatgt agcatttaaa
    39061 attgctagtt tagtagcttc gctttcgata agttgtcttg ctcttaaaga ttctacataa
    39121 aattcttgta tgttatgatt tttatagatt tcaccatcat caaataggtc aactttttta
    39181 aatccttcaa ctataggact tgaataaggc acagctctaa gtattccttc cttacagagt
    39241 atatttatgg catttcttac aggtattcta cttatgttaa ataggtcagc aatctttctc
    39301 tcacttggta atttactatt aattttaaaa gttccattta gaatgtttaa tttaatataa
    39361 tcaataattt tttcagtggc atttaagctt ttcataaaaa ctcctaaaca tataattttt
    39421 ttatgattgt attatatcat agttaagagt taaatctact tttttataat atgtataaga
    39481 caggatagag gccaaaatag ctataatagc caagataaag tatccatatt gtattgaagt
    39541 atattccgat attttaccaa ctatcattgg aaatacagac attcctatag cataaattgc
    39601 ttgaaaaaaa cccattgcag tagatttctg atgttttggt acatttttca tagactcgct
    39661 agtaaggtaa gaaaggagta tacctgtaga tatacctggt agtacttgta gtacaaatat
    39721 tatattaata ttttttattg ttggaactaa aaagcaatag gctgaaacta aaataaaaac
    39781 taaaggaatc caaaattttg gtccatactt agtacaaaat ttagtagaag caaagccaga
    39841 agaagcaaca gcacacacca tgtaaaatat agaagataga cctatgaggg tagaactaga
    39901 acctaagttt ttaagaattt gagtagtaaa tgacattgtt gtagacattt gaataccctg
    39961 ttgaattaat gccataaagg aaaaaaatac aagtctttta tccttgcaga tagataaata
    40021 ttttttaatt gatatttttt caggtatttt tttagaatta tgatttttta tatttaaggc
    40081 tagaatcgtt ccaataactg aagacaatac actcatgata cataaggctg tcattcctaa
    40141 tttttcataa aataaagtac tcataacaaa tccaatcaac attcctagat tgtttaccaa
    40201 tattatcgta cttgtagctt tttgttgatg ttctgtagaa aaaaagttgg tatagtgaac
    40261 catgtaagaa atccaagttg atgatgatat tccagataaa atgttagcta taagaaaacc
    40321 attgcctcca ggagaaaaga ctcttataag agatgctaaa ccagcaaaga aaattccaga
    40381 cagtataaat attttatgtt tatttatgga atcagcaaat actccaacag gaagccttaa
    40441 taaacattgt gatattccat atgcacctat tattatacca atcatatttg tagatacatt
    40501 acatgcactt aaatatggat tttggaaagg tatatataca tattgtgaaa accagaaaaa
    40561 tgtaacaata gaaagtaaaa gtaatttttg attagatatt tttttcatat tatcctccat
    40621 gtggtatttt tataatgcaa ataatatacc atttagattt tttaatcaag tactttctct
    40681 attaaaaaaa atttaaatat ataattatta tttacaaaaa aattatactt tatagttgaa
    40741 ttaagaaaac gcttatgata ctataaagtt aacaaatttg aatatataac agtagacgtg
    40801 ttactggtaa tgcaggcaag atcgaatcaa tttttataga ttatctatag agtactatgg
    40861 gtataatcta tttatttggt tcgatttttt tgtttaattg gccataaaac aaaaagccta
    40921 tagttgaaaa tggaggattt tttatggatt ataaaaatat agctcaagaa attctactaa
    40981 atgtaggagg aaaagagaat gtaaatgaag tgactcattg tatgacaaga cttagattta
    41041 aagtcaaaag cgcttctaaa gtgaataagg ataaattatc taaaactgaa ggagtaataa
    41101 ctgttgttga gagtatgggt caaatacaag ttgtaattgg aaataaagta aaaaaagtat
    41161 atgatgaggt cataaaaatt gtacctcaat caaataacat aggtaaaaag aatgaaagtc
    41221 aagaaaagca aggtataatt aattctatat tatcagcagt agctggtata tttacaccta
    41281 caatacctgc tatagcaggg gttggtatga ttaaaggtat tttatcagtt cttgctatgt
    41341 attatatgaa taaaaatgga gtagatataa aagaaacgca aagctatatt atcttaaatg
    41401 ctatggctga ttctatattc tattttatgc ctattatact aggatatact gcggctaaag
    41461 tatttaatgc aaataaaata atatctatgg tattaggagc tacactttgt tatccaactt
    41521 ttactgcttt gatggcaggt gaagaatctg ttagattttt aggattggca gtaacaaaag
    41581 ctacgtatac atcatctgtt attccaataa ttattgctat atgggctctt tcatacgttg
    41641 aaaaagtatt agaaaagtat atacctgagg taattaaaat aataatggtt ccaactttat
    41701 cattggtagt aatgttacct gcaacattat ttttatttgg accaatcgga atttatatag
    41761 gaaatgtcat aaacttttct tataagtata tttatgaatt aagcccagca ttgtgtggag
    41821 catttgtagg tgggttatgg tgtgtgttag ttatatttgg tgctcataga gcattattac
    41881 caataggtat aagtgatgtt gctcaaacag gaagacaaaa tttattggcg tttgcaggag
    41941 ctgcaaactt ttcacaagca ggggctgctc taggagtatt ctttaaaact aaaaatcaag
    42001 gactaaaaac aatttctatg tctgcaacta taacagcttt atttggtata actgaacctg
    42061 caatatacgg tgccaatctt agattaaaaa aacctatggt atgtgccgta atatgtggtg
    42121 catttggagg tgcgataatg ggaatgggtg gagcttatgg taatgcattt gcaaaccaag
    42181 gtgttttaac tatacctgta tatgctgaag caggtgcatt aggtttctta agttatctag
    42241 gtggttgtgc tatagcattt tttggttcag ctatttcaac ttatctagtt ggatttgaag
    42301 atttggaaga gtcttctaat aaggaaaatt catctataaa agtagaaacg aaggatggtg
    42361 ttattgatat aacttcacct gtagagggcg aatgtataga attgtcagaa gtaaaggatg
    42421 atgtatttgc ttcaaaagct atgggagaag gtatagctgt gttacctaca aaaggagtaa
    42481 taacagcccc tactgattgt gaagtagctt ctttatttcc aacattacat gcaataggat
    42541 taaaattaga taatggagca gaaatgttaa tacatgttgg aatcaataca gtagagttaa
    42601 atggtaaata ctttacaaaa catgttaatc aaggagattt agtgaaaaaa ggtgataaac
    42661 ttatatcgtt tgatatagat aaaataaaaa aggctggtta tgatgtaact acgccagtta
    42721 tagtaaacaa tacatttgat tttgggcaag tagtttcttg taaatctagt tatgtttcaa
    42781 ccaatgataa tataatttca ttagtattag ctggtaatta agttaatttg actagagata
    42841 agtaaattga tttaaaagga aataagtaaa tcaacaatta aatacatcta aaagtaagtt
    42901 tattgcaatg aattgttaaa gaaaataatt aatatggagt aaatctattg caatgaagta
    42961 acaaagaaaa taattaatat agagtaagtt tattgtaatg aattgttaaa gaaaagagtt
    43021 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
    43081 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
    43141 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
    43201 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
    43261 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
    43321 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
    43381 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
    43441 agagataaac cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg
    43501 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
    43561 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
    43621 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
    43681 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
    43741 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
    43801 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
    43861 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
    43921 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
    43981 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
    44041 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
    44101 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
    44161 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
    44221 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
    44281 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
    44341 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
    44401 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
    44461 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
    44521 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
    44581 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
    44641 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
    44701 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
    44761 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
    44821 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
    44881 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
    44941 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
    45001 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
    45061 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
    45121 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
    45181 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
    45241 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
    45301 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
    45361 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
    45421 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
    45481 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
    45541 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
    45601 ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta
    45661 catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga
    45721 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
    45781 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
    45841 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
    45901 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca
    45961 ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt
    46021 tcattgagga acatattcta agtgatgtca agatactaca atatgactaa attcctagtg
    46081 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
    46141 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga
    46201 agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca
    46261 gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
    46321 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
    46381 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
    46441 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
    46501 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac
    46561 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc
    46621 aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
    46681 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
    46741 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
    46801 cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt
    46861 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
    46921 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
    46981 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
    47041 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
    47101 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
    47161 cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga
    47221 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
    47281 gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa
    47341 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
    47401 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
    47461 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
    47521 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
    47581 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
    47641 ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag
    47701 attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc
    47761 atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa
    47821 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
    47881 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
    47941 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
    48001 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
    48061 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
    48121 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
    48181 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
    48241 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
    48301 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
    48361 ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt
    48421 cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa
    48481 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
    48541 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
    48601 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
    48661 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
    48721 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
    48781 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
    48841 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
    48901 cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt
    48961 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
    49021 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
    49081 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
    49141 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
    49201 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
    49261 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
    49321 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
    49381 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
    49441 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
    49501 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
    49561 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
    49621 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
    49681 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
    49741 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
    49801 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
    49861 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
    49921 tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt
    49981 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc
    50041 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
    50101 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
    50161 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
    50221 tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
    50281 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
    50341 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
    50401 gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt
    50461 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
    50521 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
    50581 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
    50641 aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat
    50701 agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa
    50761 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
    50821 actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
    50881 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
    50941 cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata
    51001 tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
    51061 ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
    51121 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
    51181 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa
    51241 aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
    51301 ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg
    51361 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc
    51421 ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt
    51481 agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata
    51541 tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa
    51601 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
    51661 tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa
    51721 tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt
    51781 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa
    51841 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat
    51901 ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat
    51961 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
    52021 ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct
    52081 tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa
    52141 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa
    52201 gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa
    52261 tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
    52321 agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
    52381 actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa
    52441 ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat
    52501 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
    52561 gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
    52621 caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga
    52681 aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca catgtcattt
    52741 ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca
    52801 gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat
    52861 atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
    52921 gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc
    52981 gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata
    53041 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    53101 cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa
    53161 aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt
    53221 ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt
    53281 gctctttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    53341 acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca
    53401 gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt
    53461 tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    53521 atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    53581 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    53641 actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga
    53701 aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt
    53761 gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt
    53821 tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt
    53881 tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa
    53941 gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga
    54001 tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag
    54061 taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg
    54121 tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt
    54181 tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt
    54241 tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga
    54301 aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta
    54361 aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa
    54421 atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg
    54481 attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta
    54541 caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag
    54601 atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag
    54661 atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg
    54721 tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag
    54781 ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag
    54841 atattttcta tagaaaatat cttttttgtt acgtttattt taagagaaat caatatactt
    54901 ttatatgaaa tattgtaaaa aaatttattt ttaatgagaa tatattttca ttaataaaca
    54961 attttattat gtattaataa tgtta
//
LOCUS       DAYAOY010000015        53397 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYAOY010000015 DAYAOY010000000
VERSION     DAYAOY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.015"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(301..618)
                     /locus_tag="ACGI6B_000992"
     CDS             complement(301..618)
                     /locus_tag="ACGI6B_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750457.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            complement(725..1228)
                     /locus_tag="ACGI6B_000993"
     CDS             complement(725..1228)
                     /locus_tag="ACGI6B_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9750458.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            complement(1280..2479)
                     /locus_tag="ACGI6B_000994"
     CDS             complement(1280..2479)
                     /locus_tag="ACGI6B_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HFG9750459.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(2739..2927)
                     /locus_tag="ACGI6B_000995"
     CDS             complement(2739..2927)
                     /locus_tag="ACGI6B_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750460.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            3262..4230
                     /locus_tag="ACGI6B_000996"
     CDS             3262..4230
                     /locus_tag="ACGI6B_000996"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFG9750461.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(4347..5477)
                     /locus_tag="ACGI6B_000997"
     CDS             complement(4347..5477)
                     /locus_tag="ACGI6B_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9750462.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(5630..6583)
                     /gene="msrA"
                     /locus_tag="ACGI6B_000998"
     CDS             complement(5630..6583)
                     /gene="msrA"
                     /locus_tag="ACGI6B_000998"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFG9750463.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(6707..7612)
                     /locus_tag="ACGI6B_000999"
     CDS             complement(6707..7612)
                     /locus_tag="ACGI6B_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9750464.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(7984..9576)
                     /gene="hcp"
                     /locus_tag="ACGI6B_001000"
                     /gene_synonym="priS"
     CDS             complement(7984..9576)
                     /gene="hcp"
                     /locus_tag="ACGI6B_001000"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFG9750465.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(9636..10361)
                     /locus_tag="ACGI6B_001001"
     CDS             complement(9636..10361)
                     /locus_tag="ACGI6B_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9750466.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            10526..11242
                     /locus_tag="ACGI6B_001002"
     CDS             10526..11242
                     /locus_tag="ACGI6B_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9750467.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            complement(11367..11942)
                     /locus_tag="ACGI6B_001003"
     CDS             complement(11367..11942)
                     /locus_tag="ACGI6B_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9750468.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(12116..12538)
                     /locus_tag="ACGI6B_001004"
     CDS             complement(12116..12538)
                     /locus_tag="ACGI6B_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9750469.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(12552..14729)
                     /locus_tag="ACGI6B_001005"
     CDS             complement(12552..14729)
                     /locus_tag="ACGI6B_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFG9750470.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            15010..18189
                     /locus_tag="ACGI6B_001006"
     CDS             15010..18189
                     /locus_tag="ACGI6B_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFG9750471.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(18474..19238)
                     /locus_tag="ACGI6B_001007"
     CDS             complement(18474..19238)
                     /locus_tag="ACGI6B_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9750472.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(19394..19681)
                     /locus_tag="ACGI6B_001008"
     CDS             complement(19394..19681)
                     /locus_tag="ACGI6B_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750473.1"
                     /translation="MLCYRNEVAKNTIEVVKDLKAEDMKRKVSPIDLERILVTGGVIK
                     HEDSIWLLDFWGNKDIAGILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(19701..21194)
                     /locus_tag="ACGI6B_001009"
     CDS             complement(19701..21194)
                     /locus_tag="ACGI6B_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9750474.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(21522..22064)
                     /locus_tag="ACGI6B_001010"
     CDS             complement(21522..22064)
                     /locus_tag="ACGI6B_001010"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9750475.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            complement(22354..23301)
                     /locus_tag="ACGI6B_001011"
     CDS             complement(22354..23301)
                     /locus_tag="ACGI6B_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750476.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(23329..23820)
                     /locus_tag="ACGI6B_001012"
     CDS             complement(23329..23820)
                     /locus_tag="ACGI6B_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750477.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(24263..25156)
                     /locus_tag="ACGI6B_001013"
     CDS             complement(24263..25156)
                     /locus_tag="ACGI6B_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9750478.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            complement(25381..27666)
                     /locus_tag="ACGI6B_001014"
     CDS             complement(25381..27666)
                     /locus_tag="ACGI6B_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFG9750479.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            complement(27663..28343)
                     /gene="pgmB"
                     /locus_tag="ACGI6B_001015"
     CDS             complement(27663..28343)
                     /gene="pgmB"
                     /locus_tag="ACGI6B_001015"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFG9750480.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            complement(28567..30450)
                     /locus_tag="ACGI6B_001016"
     CDS             complement(28567..30450)
                     /locus_tag="ACGI6B_001016"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFG9750481.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            complement(30708..31529)
                     /locus_tag="ACGI6B_001017"
     CDS             complement(30708..31529)
                     /locus_tag="ACGI6B_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFG9750482.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            complement(31720..32073)
                     /locus_tag="ACGI6B_001018"
     CDS             complement(31720..32073)
                     /locus_tag="ACGI6B_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750483.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            complement(32115..33557)
                     /locus_tag="ACGI6B_001019"
     CDS             complement(32115..33557)
                     /locus_tag="ACGI6B_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9750484.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            34233..35444
                     /locus_tag="ACGI6B_001020"
     CDS             34233..35444
                     /locus_tag="ACGI6B_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9750485.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            35536..36051
                     /locus_tag="ACGI6B_001021"
     CDS             35536..36051
                     /locus_tag="ACGI6B_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HFG9750486.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(36318..38417)
                     /locus_tag="ACGI6B_001022"
     CDS             complement(36318..38417)
                     /locus_tag="ACGI6B_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HFG9750487.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(38541..39080)
                     /locus_tag="ACGI6B_001023"
     CDS             complement(38541..39080)
                     /locus_tag="ACGI6B_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9750488.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            complement(39083..39823)
                     /locus_tag="ACGI6B_001024"
     CDS             complement(39083..39823)
                     /locus_tag="ACGI6B_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9750489.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            complement(39825..40874)
                     /gene="vorB"
                     /locus_tag="ACGI6B_001025"
     CDS             complement(39825..40874)
                     /gene="vorB"
                     /locus_tag="ACGI6B_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9750490.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            complement(40891..41109)
                     /locus_tag="ACGI6B_001026"
     CDS             complement(40891..41109)
                     /locus_tag="ACGI6B_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9750491.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            complement(41142..42245)
                     /gene="hisC"
                     /locus_tag="ACGI6B_001027"
     CDS             complement(41142..42245)
                     /gene="hisC"
                     /locus_tag="ACGI6B_001027"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9750492.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            complement(42348..43778)
                     /locus_tag="ACGI6B_001028"
     CDS             complement(42348..43778)
                     /locus_tag="ACGI6B_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9750493.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            44436..45800
                     /locus_tag="ACGI6B_001029"
     CDS             44436..45800
                     /locus_tag="ACGI6B_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFG9750494.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            45824..46477
                     /locus_tag="ACGI6B_001030"
     CDS             45824..46477
                     /locus_tag="ACGI6B_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9750495.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(46596..47378)
                     /locus_tag="ACGI6B_001031"
     CDS             complement(46596..47378)
                     /locus_tag="ACGI6B_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9750496.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(47495..48661)
                     /locus_tag="ACGI6B_001032"
     CDS             complement(47495..48661)
                     /locus_tag="ACGI6B_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9750497.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            complement(48800..50164)
                     /locus_tag="ACGI6B_001033"
     CDS             complement(48800..50164)
                     /locus_tag="ACGI6B_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFG9750498.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            complement(50476..51000)
                     /locus_tag="ACGI6B_001034"
     CDS             complement(50476..51000)
                     /locus_tag="ACGI6B_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9750499.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            complement(51050..51547)
                     /locus_tag="ACGI6B_001035"
     CDS             complement(51050..51547)
                     /locus_tag="ACGI6B_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9750500.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            complement(51780..53051)
                     /gene="hflX"
                     /locus_tag="ACGI6B_001036"
     CDS             complement(51780..53051)
                     /gene="hflX"
                     /locus_tag="ACGI6B_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFG9750501.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
BASE COUNT        17767 a         8963 c         5708 g        20959 t
ORIGIN      
        1 acattcctaa acgttccctt gaaaatagcg taaattggta catctctaaa taaaattatt
       61 cttataatca ttaaaatata taccaaaaat attatgtata taattgacag taacaatttt
      121 tctgactttt tactttctat cactgataag tctcctttcg ctattaatat tttaagtata
      181 tcaataaata tttaagacga ttttaaaatt tccttaaaat ttccttaatt ttcaaacaaa
      241 aaatcaaaaa agcctacact taaatgtgta gactttaata aatttttata caaatacatt
      301 ttattgaata acgaacaatc ttaatgtaga aaaaactata actacagtta aaagaagtct
      361 agcatataaa aaattttttt ctttcaacat aactcctgca actccaaata taaaaaatag
      421 agaagcaata aattttccaa taagattatt attaggataa taactaaaaa gcataaaaca
      481 aagtacaaat gtaagtactc ctattgcatt aaacacaatt ttattagatg ctatttttcc
      541 tttattgaaa aagaaacaga atattgacac taaatctaaa aataaaaata cataaaacat
      601 tccttttaaa acgcccattt ttataccccc taaaaattct aattataata tccagtttat
      661 ttcatataat cgtcaatata atcaatactt tataacatca gtataacagt attacaaatt
      721 tatattacga atgtactact tcgtattcat acatattata atcacactca tacatattta
      781 aagactcata tgttgctttt gcatttatgt tttctttttc tacatataat ctcattccaa
      841 ctatattttc atctttatca catatatttt ttatataatt aaataaataa ttaaagatac
      901 cttttcttct atattcctta tctacatata cactctgaat ccaaaggaaa tttccatttc
      961 tccaatcact ccactcatat gtatacataa tctgtgcaac aactttatca aatactgtat
     1021 atacatgata ttttcctttt ctctcatcta ataacaatgc ttttacacct ttagttaaaa
     1081 catccatatc aagttctttt ccttcagttt ctttagccaa attataatta aattttgcta
     1141 ttgtatccaa atcatccata cgtgcttctc ttatctctat tagttcttca ttttcatata
     1201 actctatgta gtccttttgt tgctccatac ttattcctcc atatctattt tatctactaa
     1261 ggttttttat ataatatatt tatagagttt tttctatctt cataaatgca taatcaagtg
     1321 tagcctctaa atttccatta ttcttacctc ctccttgagc taatacttta cttccaccac
     1381 ccttgccatc tataagattt attgcatcct ttaataaaac tcccatatta atatctttta
     1441 aatttttaga acatgcaaaa actaaattta ttttatcatt aaatttaaca gctaataatg
     1501 caatactatt ttcatattct gcaattttat ttgctatctt atttacatat ttcaaatctt
     1561 catcatcata aattttttta atgaccgaaa tattgccaac cttatttgct ttttcaatca
     1621 tttctttgac ctgatatgta gatactactt cttcaagcct tcttttttta attaaagcct
     1681 cttccacttt actatttaga tttacaatac catttatagc atcttcctca ctacagctta
     1741 aatatctaca tattttagtc aaatatatat ccctcttcaa tacttcatcc actgcccttt
     1801 tacctgctag aaactctatt cttgtacttg acttatgttt ttcccatttc tttattttta
     1861 tcattctcaa ttcaccagtt gattttggat gcactccaca acatgcattc gtatctaaat
     1921 cacctatttt tattactctt atctcttcat cagtatttgg caaatcacgt cttaaccaaa
     1981 tcttttttag ttcttttttc gttggaagta atgtctctac ttgtatattt tgacgaataa
     2041 tttcattggc aaacaactct atctctctaa ttttatcctc ttctaaatta ccttctatat
     2101 ctactgtact atactcattt cccaaatgaa aacctgtcgt atttgcatta aaagttttaa
     2161 aaaaacatcc agacaataca tgttgtccaa aatgttgatg cattccatct tctcttctat
     2221 cccaatcaat actacacttt accttatgta tctttatagg ttttttatct aatacatggt
     2281 atattttttt tccatcttca tacacattta ttacatcatc attctctatt tttcccaggt
     2341 ctccaaactg acctcctcca ccaggaaaaa aagctgtctt atctaataaa acatgatact
     2401 tattttctac ttcttttatt tcaattattt ctgctgtaaa atctcttata tactgatttg
     2461 tataatataa tttttccatt ataattctat ctccattttc ttaattctag attactttac
     2521 tttttagata caaatttcaa actaagctta atttaattgt attaaaatag aactcatcct
     2581 attatttact aacatcttct actatatttt aacatataga tattaatgtt aacaaattta
     2641 taattgtata ttatttcaat attttataac atgtatgttt acttattttc ttatataata
     2701 aaatgtatat taaaacatgt taaattgcaa aataagcttc aatctctttt actaatttca
     2761 tccattatta atgattttga aatatacatt acttctataa tattcttaaa acgtgtatga
     2821 tgagatgtct cctcagaaaa tttcttctgt ccttttccac aattaataat acttgaagat
     2881 actactctca gtgcttccac caattcttct tttgtatgat aattcataac acatttactc
     2941 ctttattttt taatttagag aaacagtata tctcaattta atatcattat aaactgacat
     3001 cattattatt tatacacatc ttttagcact taaatattct aactatcagt gacattacat
     3061 attttaataa ttattaatat acatataata ctttaatatt cactaataga gagtatatta
     3121 acattgcttt tttaaaaatt ttagtataaa atcatattaa ataaattttc tgaaataata
     3181 gaatattaaa atatccaaaa acagctttta ataacatcta taattttcaa tgtatcgtta
     3241 ttttaggggg agtgatttaa aatggcaata aagccaagaa aaatatctat tgtaggttca
     3301 gggcatgttg gttctcattg tggatttagt ttaataactc aaggagtttg tgatgaactc
     3361 tttatgattg atatagatga atctaaatca aaagctcaag ctttagattt agctgatgca
     3421 gtttcatatc tgccacataa agtacatata gaaaaaggaa ctttttctga ctgtaaagat
     3481 tctgacatag tagtgataag tgttgcagac tcttcagaag ggcctttacg tagacaaaac
     3541 acaactagat tagatttact aagaccaact attggaatga taaaatctat agtaaaaccc
     3601 atagttgatt ctggctttga tggtatattt gtagtcatat ctaatcctgt tgatgtagtt
     3661 acaaattata tttgggaaaa atctggcttt cctaaaaaca aagtcattgg aactggaaca
     3721 gctttagatt caacaagact tagaaggatt ttatctgaag aaactggaat cgctcaacaa
     3781 tcaattcaag cttattctat gggtgaacat ggagattctc aaatggttcc ttggtctcat
     3841 gtttcaattg gtggtaaacc aatcttagac atgataaaag acaacccaag tacttatagc
     3901 aatttagact taccatcaat agttgaaaaa aataaaaaaa cagggataag tataataaat
     3961 ggtaaagatt gtacagaatt tggtataggt actgctttag tagaaattgt aaaagctata
     4021 ctacacaatg agaaaaaagt tcttccagtt tctacattac tagaaggtca atataacgaa
     4081 agaaatgtat ttgcaggtgt tccatgtgtt ataggaaaag atggtattga agaaattatt
     4141 gaaatcaata tgactgaata tgagcaaaat gaatttaata aatcttgttc tgttttaagg
     4201 gaatatattg cactttcaaa aacactttag attcaatatt ctttcttaca ctaaaagcag
     4261 gtaaatgact tattgaaaag acctttaact aagttaatgt ttaaatcatt tgtacctgct
     4321 tttaatttta atactaaaat ataaacctat tttttaccat caaattttat aactttactt
     4381 tttttattta cctcttgaga taacaatcct tttttcaatc ccatcaataa ttctaagtct
     4441 tctggataaa gatttattcc aaattcctct aaacttgata ctatactatt tacactcatt
     4501 attttatttt ctatcaataa ttcaactgct ctttttaaat acgttgggct tgaacctttc
     4561 attttgtcta gtggttcttc tatcaaccag ccttttttat ccatttcatt cataagataa
     4621 ttgtattttt tgtaatttat cttttctaga ttatatgctc taaaaatcat agcagatata
     4681 ggtacaatcc attttttctt tagttctaca taataatcta aatcttctgg atgagtcaaa
     4741 tcttttaaaa aactctcttt tggcaataaa aatgcacagg catactcatc ttctgaaaat
     4801 tgcttacttg gtattcttaa ttcacttgat attatatatc caagctcaca tgccaaagta
     4861 taatttctaa ttgatgctga ctttttgtca ttacccagag atattagata tttagattct
     4921 ttactaattc tctgtttttg agtaaagata gttgcccctt ttctatctac attcatacct
     4981 gatattataa tccctttaga ctctaacaaa cttaccatat ttactattgg agtactattt
     5041 aaatcccaaa actctctagt cttatatgca agagattcta tatcatttat attaatatct
     5101 gacattactt ttctatttgg caagttttct tttggaaact taatataatt ctccataaat
     5161 gaatatattt tatgtattat aattattttt tctctataag aaatctcttc tactctaggt
     5221 agtttagatt gagggttaaa gtggctatcc tctacaacga tatttatatt ttcattttta
     5281 taaaaatact cttttggaaa atttaatata ttagataatt ttaatgtatt ttctatattt
     5341 ggtacatact tattttcctc aaaagctttt aaatcttttt tatttatttt agtttcttta
     5401 gacaactgtt ctatcgtctt tgctctaaaa attctagctg attttaatct ctttccatta
     5461 aagtctttta aattcatttg ttttctctct ttcaaaatta aattcttaat ctattggtat
     5521 aaataagatt acaattaata atttatcata ttattgacaa aaaaacaaaa ggtgaaaata
     5581 tttttttata ttttcacctt ttagataagt ttataataaa tattcctaat tactcaaaaa
     5641 gttttaaata ttctccatat cctaaagctt ccatattttc tcttggtata aatttaagag
     5701 aagcagaatt tatacaatat cttaaacctc caagttcttt tggaccatca tcaaatacat
     5761 gacctaaatg agaatttcct ttagcacttc ttacttcaac tctgtacata ccttgtgaga
     5821 aatcacttct ctcaatgata gatttatcta taactggctt tgaaaaactt ggccacccac
     5881 atccagaatc aaatttatct tttgaagaaa ataacacttc tccacttaca atatcaacat
     5941 aaattccctc tttgttattg tcataatatt cattttcaaa aggtacttct gtcatatcat
     6001 tttgagtgac ttcaaattgt ataggagtta gagtcttttt taactcttcc ctatttatat
     6061 tgtttccatc ccaatggctt tttacaaaat tatatcttcc agacccttta taatacatat
     6121 tgtagtttaa tggtcttttc ttataataat cctgatgatt ttcttctgct tcgtagaatt
     6181 tagttgctgg aattatatca gtcactattt tactatcaaa tattttacta ttttccagat
     6241 gttctcttga ctctaaagct atattttttt gttcttcatc atgataaaat atagcagtct
     6301 tatattgagt tcccctatca ccaaattgtc caccatcatc tgtaggatta atctgtttcc
     6361 agaagatatt tagaagttca tcataactta ttatatcatc atcatatgtt atttgaattg
     6421 cttctaaatg accagtagtt cctgagcata cctcttgata tgtagggttt tcaacatgac
     6481 cacctgtata tccagaaact actttaataa ctccatcata cttatcaaaa ggttttacca
     6541 tacaccaaaa acatccacct gaaaaagtag caagttttat cattttcatc actccttaaa
     6601 taaattatac tttatttatt ccctaagaag taacttaata atctgcattt tattattttt
     6661 acctactcaa tgaaatcatg tgtaaaaata caattgcaaa ttgaatctaa tttctataaa
     6721 agctcttttt ctttatccat ccaaaagcat cttttatatc acaattttct cctaaagaca
     6781 ttagatattc taatgcatca caatcatcca tagctacatc tttttccatc ttaggattta
     6841 atctatagta attatctcca agaagttttt gagaaaataa ctgactcatt tgagaatttc
     6901 cttctaaggt aatagatata attggtaaat cgggttcttt attaattatc cagtcaatag
     6961 ctccccattc ggtagtatct gcctttatac tgttatatac atatccagtc cctattgaaa
     7021 aaagtcttgt attcttaagt ttaaatccac tgtcaatggc atgaattata cacgctaaac
     7081 tcgggtcagt tgctattatt cctccatcaa catgacgatt atatgttgga aaaaataccg
     7141 gagcagcact acttgccatt gctacatcta caactttgct agtctttgta aaagaatttg
     7201 gcatattgtt ataaaacact ggtttccatg cattttcctc atttcctata taaaaacttg
     7261 gtatcattac taatttattt aaatctttta attctagttc ctctggaaat atacttaata
     7321 aaacttcttt taagtttttg ttttcatact ttggtctgag tatttctgca taacttttat
     7381 taaaaatata ctttgaattt tcaattgagt aaagttcttt tatctcttta gggcttacac
     7441 catacgcaag ccctagtgca attagactcc ctgtagatgt acctccaaac atatttatat
     7501 tgtttaatag atttggatac tcttttacta tcctctcaag tatggatata ctcaatgccc
     7561 cttttaatcc accaccatca aatgccaata tattaaaatt agaatttttc atatacatat
     7621 acccccacat aaaacacctt atattttaaa ttttaatcaa aataatttca ctatagtata
     7681 tatattttat gcaagttaca tattacaatg ttcctctttt aatatttaag ttacattcaa
     7741 acctttaata cttaaaataa aatatataag atattatata tctaagtatt ataaatttaa
     7801 tacaaagatt cttaaaaaga atatactgaa agcaggttta tttttcaatt attacaaaaa
     7861 ataaataaag ggtcatctca aaattgattt tttaatcaaa ttttaagttt aaccccttat
     7921 cttaattgtg gcatacttaa aaatatttta actataaata tctttaagta tagatatgtt
     7981 tatttatcct aaaatcttct tcatatcttc ttcaggagta gatataggtg tcaatgcaaa
     8041 tttatcaacc aatatatcta atacattctt agataagaat gctggcaaag aaggccctat
     8101 atagatattt ttgataccta aatgaagtag tgtaagaagt atacaaacag ctttctgctc
     8161 ataccatgac agtaccatgc taagaggcaa atcatttact ccacattcaa aagcatttgc
     8221 caatgaaact gccactttaa ttgcactata agcatcatta cattgtccca tatccattaa
     8281 acgaggtaag ccacctatat ccccaaggtc taaatcatta aatctatatt ttccacatgc
     8341 aagtgtcaat ataacagtgt ctttaggagc ttgttttaca aattcagtgt aataatttct
     8401 tccaggtttt gcaccatcac aacctccaac caataaaaag tgtcttatag ctccactctt
     8461 tacagcatct actactttat cttgtacaga tgaaactgta cctcttgcaa aaccagtagt
     8521 taatgtatct cctccattaa taccagtcat atgaacatct tctctatatc ctccaagttc
     8581 aagagctttt tctattacac tagaaaaatc tttatcttca tcaatatgaa ccatacctgg
     8641 ataagcaact acttctgtag taaatactct atctctataa ctatctttaa caggcattaa
     8701 acagtttgtt gtaaataata ttggagcagg aatattatca aattcttttt gctggttttg
     8761 ccatgctgta ccaaaattac cttttagatg tttatattct tttagttttg gatatgcatg
     8821 tgctggtaat aactcaccat gagtatatat atttataccc ttatctttcg tttgttctaa
     8881 taatttgtgt aaatcataaa ggtcatgtcc tgtaactaca ataaatggcc ctttctcgat
     8941 tttcatagat acctgagtcg gttctggtgt tccataagtt tcagtatttg ctctatcaag
     9001 caattccata cattttaagt ttatttctcc agttttcata actagaccta aaagttcgtc
     9061 aatagacaag tctcctccaa ctgctattaa tccttcatag aaaaattcat ttacgtcttt
     9121 gtctgtataa ccaagtacca ttgcatgata tgcataggca gccatacctc ttaatccaaa
     9181 taagattaat gattttaatg aacgtatgtc ctcattatca ttccataaat ccttcatatc
     9241 atagttatca tttctattac aagttgttga acaatctgaa catctaggta caagtttact
     9301 tttttctacc tcagtttttt caatttgtct ttttatagct tcatcatcaa aacttacatt
     9361 cgttatagta gtgaatagcc cctctatcat aattttatct gtttcacttg ttggtctatt
     9421 cttatttcca atagttgctc ttgaaagacc aatcaacact cctgtaagtt cgtcttgtaa
     9481 gttagctgta cccccctttt taccacagac accatttata gtacaacctt tacctcctgc
     9541 tgtttgttca cattgaaaac aaaacatttt ttccattttt tattcctccc tttttattta
     9601 ttaaaattta ttatattttt ctataattca cttaattaaa tttctccatc tacaattttt
     9661 ccatctacag atatagtaac tatttgccat ggtatcattt ttccactatt ttgtagagct
     9721 gtcttaacag catttactat accaccacag caaggaactt ccatacgtgt tactactata
     9781 tttttaatat cattttcttt taaaatctct gtaagttttt cagcatagtc aacttcatca
     9841 agtttagggc atccgattaa tgtaacctta tttttcataa atttattatg aaaatttcca
     9901 tatgcatacg ctgtacagtc tgctgctatt aaaaaacttg cattctttaa atatgatgca
     9961 ttagttggta ctaattttat ttgtactggc cattgattaa gttgagaacc ttttatctct
    10021 actatatcat tgcttataga cgtactattt gaaacctctc tatttattga cttagactga
    10081 gaacctggac atccacatgc taaagttttc ttttgtgctt tctttgcttc catatttgct
    10141 tttactgcag cttcatcata ttctgctgct tctctatact caaatgaaat cgcatttgtt
    10201 ggacaagttg gcagacaatc tcccagccca tcacaatagt catctcttaa aagttttgct
    10261 tttccattta ccattcctat agcttcttca tgacaagctt caacacatag tccacaacca
    10321 ttacactttt cctcatcaat tttaattatt tttctcatca tttatatcac ctcataaaat
    10381 ttttttaatt taaagtattg atttcttata tatttatttt taatacctat atttctttat
    10441 ttcaaatttc ttgtagtagg atttttatat ccccttgctt ttataaactt agtatagtac
    10501 aatgaaaaca aattatcggt tgcatatgca acaaggaagt gattatttga aaaaattttt
    10561 agaaaagcat atagacttac ttaaaaaaat tcctctattt gaaggaatta aaagtgatga
    10621 acttgaagaa atgttggaat gtttaggtgt tgtagataag acttacccaa aagatagtat
    10681 aatattctct gttggaagtg aagttacttc tattggaatt atgcttaaag gaagtgctca
    10741 tataataaag gaagatatcg aagggaatag gaatattgta gctgaactct tgcctggaga
    10801 tttatttgga gaggtattcg catgtactag actacataaa agtaatgtta cagtaaccac
    10861 aacatcttct tgcgaagttt tatttataaa gtttaaaagt gtaactggta tttgttcatc
    10921 tgcatgtgta tttcataata ggcttgttga aaatatgcta caattaatag ctgaaaaaaa
    10981 cattttactt cacaataaaa tagaattact ttctcgtaaa acaactcgcg agaaattgct
    11041 tatgtatttt tcaaaacaga tagaacaaac aggtagccac cagtttacaa ttcccttttc
    11101 aagaaatgaa atggctgatt ttttatgtgt tgacagaagt tctatgtcaa gagagcttgg
    11161 caagatgcga gatgaaggct tgttatcttt taacaaaaat aaatttgaaa tcttttattt
    11221 tcaaaacaag ttagatatct aggcttgtat aatatctgta ttcaaataat attgtttttt
    11281 attagttatt ttctagaaac ctttatacaa aaaaggagta ttaataatta atgctccttt
    11341 agctatacta taaattaaac ttttatctat ccattgatat caatggctat tttcattcca
    11401 agtttatagg cttttttcaa taccttaaaa ttttttttag tggaatttct ttcaaatgct
    11461 ttggttacaa ctatagtttt gtagtgattt aatcccatgc cctttaaatg ttttgcagta
    11521 tacttaaaat attttttaaa taaaaacgaa ataggagctg cttgagtata gaccatgatt
    11581 aacttttttt gaccaaacct aggtatatgt ttagacttat ttatctctgt taaaggatac
    11641 aatctatcta aaagtagctt tgtttgagct gatacctgac atatatatac tggcgaacca
    11701 attattatat attcagcact ttttatatct tcatatatgt caatcatatc gtctttaaat
    11761 gcacaagtag gaacttttct acagtataga cagccttgac atcctttaat attcatatca
    11821 tttagataat attttttaac ttcaacatta ttatcattta caccttttat tatctcatca
    11881 agtattgtgt ctacattccc tttttttctt ggactgccca taaaggctaa tacttttacc
    11941 atattaagtt ctcctgtcta atttattttt ctaaatttaa tctaaagcac actttattct
    12001 aaaaatatat tttatatact ttgtatttgt tttatttaca ttttatatta caaaagtata
    12061 tcaaaaaata attcttaaca caattattta aaagtagtat agcaatttaa tacaattatt
    12121 tattaaattt taatgctcct gtagggcata ccattacaca aggtgaatcc ataccacact
    12181 tatatctctc aatacaacca ttacacttat ttgccttacc atcatttcca taaattaagg
    12241 ctccatattc acaagctttt tcacaaagtt tacaccctat acagttttga ttgtctaata
    12301 caatgaatcc ttcttctttt ttaaaacaat ttttaggaca tacacctaaa cattttggct
    12361 cattacaatg tatacatgct aatgaaaaat attctatatt ttcaccttcc tctattgtaa
    12421 atacatgtct aaaaagttca ccattgtcta tattattatc attttggtct atacatgcaa
    12481 gcaaacatgc tccacatgct gtacataatt ctttattaaa tttaaataaa cttttcatta
    12541 tatctctcca tttatctatt cattttttca atcttacact tacaacttct atacgcaggg
    12601 aatccactat aagggtcaaa attatcacaa ataatactat ttatatcagc ttctctatat
    12661 ccatgataca tatgtatatt cttatataag actctatcag taacattgac ttttacatcc
    12721 aaacttccta tcttagtagt tatttttata taatctccac tgataacacc caattctttt
    12781 gcatctaagc tagaaatatc acaattagct tcatttttta atattcttga aagtttgatg
    12841 tcatgaagtc ttgaatgtag tgcatttgaa attcttgctc ctgatgtcaa taaatgagta
    12901 tattcatcat ctatattatc attaaatcct ctatatgttg gtagtgcatc cagcccaaaa
    12961 tctttatatt tttcaataat tgtagatttt aattcaaacc tagaagtgct agtatcatac
    13021 ccttctcttg tatatgaacc tactatatat ggcttaaaat ctttacattt taatggtaaa
    13081 tcactttctt taagtttatc tacatctaca catgtatttt taaacatatg tcttacactt
    13141 gcttcataac ctgcttttaa taaatcatca tctatattca tagcatttgc taaatctgaa
    13201 agtatttcta catctgattt tgaatcgtat aatttatcaa taactttttt ggtatatgta
    13261 atataccctc ctggataact cttacattct tctctctcaa atgatgtaca tgcaggtaaa
    13321 agtatatctg catattttga tgtatctgtc ataaataaat ctatattcac aaaaaaatct
    13381 acactattca acgctttaaa aagtctccct gaattaggaa acattctagc atttatccca
    13441 tgacaaaaca aagctcttat ttcatatggt gtcttttctt ctatttgcct aggtaaatcc
    13501 atagcttgaa attgagtcac catttcattc catagaggaa atctttctcc accaattctt
    13561 tcatttatat ttttaggtag aacttctttt acaaactctt tttctagtgt ttggaatcca
    13621 cctatcttac cagcaaagta atcttgtttt ggtatgtttc cacctattct atcataatta
    13681 cctgttatag cggataaaga catcattgct ctataatttt gaaatccatt ttgatgatga
    13741 actattggag ctgcactttc atttatacaa aatggacctt gtgacaacat ctcaacagct
    13801 tttactatat tatctttaga aactcctgta attctactta cagtatctaa atcaaataat
    13861 tttacatatt ctttatattc attaaatcca tgtacatgct tttctatgta ttctctatct
    13921 attaaatcct tttgtataaa tagattcgcc attccaagag ctaaagctcc atcagttcca
    13981 ggtttaattc ttaaatgtaa atctgctagt ttttgagttg tcggtgttat tctagggtct
    14041 acaattataa attttagacc tttttctttt aattttatta gattcacact atttaaaaat
    14101 ttagagtagt atggattaaa tccccaccct aaataagttt ttgcattctc catatcttgc
    14161 attgtaaagt ttcttccagt agcaacattt ccagccatct gtgctgatgt tgaacatgaa
    14221 ctagattctg taccataatt aggtgaacca aatgaagatg caaacctttg taaaaaaggt
    14281 ctataccact ttccataacc cgtaaaaaaa gctacatgct ttgctccata ctcagatttt
    14341 actctattta atttttctgc tatttctcta taagcttcat cccatgatat tggtttaaat
    14401 tttccttcac ctctttttcc aattcttttt agaggtgttt ttattctatc ttctctatag
    14461 atatattctc tattgcaaag acctttggta cataacaacc ctttattcat tggatgttct
    14521 tttgtacctt ctatttttat tactttacca tctttaacat atgcatcaat tccacaatgt
    14581 actcctggcg aacaaatatc acataaagtc ctctttattt ctatacctgt ttccttacag
    14641 ggtattttag ctttaagttg tttataacta tccatattaa cattttcctt tcccaataat
    14701 aaatacaaat tattatactt attatacatt ctttttattc aaatatcata ctttatattt
    14761 tatacagtgt aatttctaag aatattttta ataataagat tttatattta ttaataatct
    14821 atttatataa ttatttttca ttcatagttt ataagataat tcaataaaaa tctaatagta
    14881 aattgttatt tacacatata tcgtgataga taaaagctat tcatatctgc cttaatttta
    14941 gtcttaaatt tagagtgtaa attgaatgca tttagattat aatatataca gtgaggtaag
    15001 gtgaataata tgaaaataga tatgatatat aaactaataa gaagaaatac agaaccatca
    15061 agatggtcaa gaggtgtaaa atattatact aatcaattag ttgattatat agacatagat
    15121 attgatgacc tacaaaataa tctgttgaca tttcgtggtg gagtcaattc tgaagataat
    15181 tatagctttt attttaattc aataactata gatataaagc actttaagat aattgaaaat
    15241 gattgtagtt gcgctgactg tcaatcaaag ggcaaaagta tttttatatg taagcatata
    15301 gttgctattg tgataaaaag cttaaaacaa ataaaaaata aaaaagatgt tcctcctatt
    15361 ctaatagata tattaggtgc agataaggat aagctttcta atacaaattc taaaaccagc
    15421 ttagatttaa gcaagcaatt actttctttg ttagacaata ctaaaagaga aagggttaat
    15481 ttagatgtat tgataaatgt ggattcaaat aaagcaattg ctgaatttaa aataggaatt
    15541 aataaaatgt acattttaaa aaacattact gaatttgcat attcaaaact taatggatat
    15601 gaattagatt ttgggaagaa atttacttac aaccccaaca ctcattattt tgctgatgaa
    15661 gatgagaagt tagtagatat tatagaagaa tatgggacaa atctttcata caacttttct
    15721 tcatcacatt caaaaaaatt tatggagata aatgcaagct ctttaaaaca atttttaaat
    15781 gctattaagt acaaggattt tacccttatt ttttctaaag aggaatatca tccaaagata
    15841 ttaaatgata ctctacctgt aaggtttgat tttaaaaata tagaagataa aatagtctta
    15901 tattctaaag attcactacc tgttccactt actaaaaaaa atgatgtact tttatatgat
    15961 ggcaatatct atttattatc acataaaaaa gctcatgatt attcaaaaat atatcaaata
    16021 ttatcaaaga ctaaagagtt aaaatttgat aaagaggatt ctaaggatgt tttaggattg
    16081 ctagttccta gattaaaatc tatatctcaa gaagttcatt tagatgataa cataaaaaat
    16141 aatataacaa aagattttaa atctgagttc tactttgata tgattgatgg caacatatgt
    16201 tgtgatgtaa agtatatata tgatgatgaa gataaaaaat ttgtgctccc caacatacaa
    16261 aaggaagcta ctattgaaaa taaacttact tcttcacaat ttacaaagga aaataatcat
    16321 tatgtgttta aaggaactga ccatgaccta tttacatttt tagatttaca attagaatct
    16381 ctaaaggaat ttggagatat atattattct gagaagttta aattgaaaaa aatatataac
    16441 gcttcatcaa ttcaagcttc aatcaatcag actaaccaga attacctaga atttaatttt
    16501 aatatatctg atataaatcc aaaagagtac aaggatattt tgaaagcttt tcaagaaaaa
    16561 cgtacttttt ataaacttaa agatggaaac tttatagatt tatcagaaag agaaacaaaa
    16621 gatttttttg aattagttga aaatctagat ataatggata aatcaaaaaa taataaaatt
    16681 cataaaaata aagctctata tataaatgat gtaatacgta gtaaaaattt aaagtttata
    16741 gatggtaaaa aagaactaga cagtatatgt gaaaaattta gaaattttga tagcataaat
    16801 ttagaaattc ccgcaaattt aaatgcaaac cttcgtgact atcaaataaa tggcctcaat
    16861 tggtttaaag ttttagatta ttataaattt ggtggtatat tagctgatga aatgggtctt
    16921 ggtaaaacaa tacagactat tgcattttta ttatcattat ctaataaaaa aagtcttatt
    16981 gttacaccga catcactcat ttataattgg aaaaatgaat ttgaaaagtt tgctccagat
    17041 ataaaagttt tattgataca tggaaataaa agagatagag aaaaatgttt tatggaatta
    17101 gaaaattttg atgtaatact tacaacatat ggtactctta gaaatgattt agacaaatat
    17161 agtgaaataa agtttgatta ttgtatatta gacgaagctc aaaatataaa aaatccagtg
    17221 gctttagtaa ctgagtctgt aaaaagtata aatgcagaaa ataaatttgc actcactggt
    17281 acacctatgg aaaataatct actagaatta tggtctatat ttgattttat aatgcctggc
    17341 tatctatata gtaaagcaaa gtttcaggaa ttgtttataa ataaagaaga taatgttaaa
    17401 aacctgaaaa aattgataaa accttttatt cttagaagaa gtaaaaaaca agttatgaaa
    17461 gaacttccag ataagattga aaaaaacttt tttgttgaat tgaataaaga gcagaaaaag
    17521 atatatagtg tatattcaaa agatatacaa gataaaatga aagataaaaa tctaaagaaa
    17581 gataaaatag ttatattctc ttatttaact aaactaagac aactttgttt agacccaagt
    17641 atagtagtaa aagattacaa taaaaaaagt tcaaaaattg aaacatgttt agaaatactt
    17701 agggatagta taaacgaaaa ccacaaaata ttattgtttt cacaatttac ttctgtgctt
    17761 aagaacataa gtaaggaact tgataagtat aaaattaaat accattatat agatggtaaa
    17821 actaatgcca aagaaagact tgaacttgta gatgagttca ataatagtat ggataaaaaa
    17881 gtttttttaa tatctttaaa agcaggcgga actggtttaa atcttacatc tgctgacatg
    17941 gtaattcact ttgacccatg gtggaatcca tcagtagaaa atcaagctag tgatagagct
    18001 catagatttg gtcaaaaaaa ttcagtacaa gttataaaat tgattgctaa agggactata
    18061 gaagaaaaaa taataaaatt acaagaaagt aaaaaagagc ttataaatca atttataaat
    18121 ggagaacttt ctaatgaagg tgtattaaaa agtctatctg atgaggagat aataaactta
    18181 tttaactaaa ttatttaatc ctacagctta actatatgaa tttgttctaa catttatata
    18241 gttaagctaa aatatctgaa taaataaaat ttaatattgt tatttaatcc caactttaca
    18301 atctaatgat atctctttct atacatattg catatacaaa tgtaataact tgactattag
    18361 aatattttgt tttaacaatc ctatttcatc ataataatga agtatatgta cactcacttt
    18421 tgtaagtttt gcaatccatt ttacagtttt cattattgta aattttggtt aaattattcc
    18481 tctaaaaatt tctttgctac atttgaaaaa tctaatccat tcgataaaat tgcaggatga
    18541 ccacctttat caaacaaata cattttacca tttttaatct tttcaagaag taatgaaaaa
    18601 gtattttcaa aaaaatcact agatattaga gatacaaact catcttctct acttcctgtt
    18661 aaaagcacct ctggttgcat agtttctaga ggcttatgaa aaaactttcc tatagtttta
    18721 taatgttcaa aaatagcttc tgtatcatta tctacaactt tttcccagtt ttctccttga
    18781 ttataaatat aaaaagcttt tgcaccatca tcttgcttag atgcttcacg ctctattttc
    18841 acattttgta caaatgactc tagaggaact tctccttcaa aactatctgc aattacctta
    18901 ttcactaaat caggtctctc aagtgcaaca tttaatgcaa caagagctcc tccactacta
    18961 ccaattatgt ttactttttt atagttcatt gcttctaaaa aacatatcac ttgcatagcc
    19021 tcatcaaacc ataagtcagc agaaaattta tcgaccattt gagattttcc atgacctaca
    19081 aaatcaatta atattacttt atattcatct ttataaaaat tgattatatc attaaacatc
    19141 ttggaagagg ctgtatttcc atgtaaaaaa agtaatggtg taccatcccc aatttcatta
    19201 tagtagcact gtttgtcgtt atagttaaaa tatgccatat atttcccccc tttaatatcc
    19261 ctataggtat atatttatta cttatattta aaatgactat tcccttagcc acttaaaaat
    19321 agtataactt agaatgtaga taaaatcaac attccttgct aatttatcta ttgtttgtct
    19381 aatttattat aatttaactt ctgtaaaatt tcttttttgt acgaaaatat tccttccatt
    19441 tacaacaatc attcaaatga agtataacat gacgagtagg aggcataagc aaaattccag
    19501 caatatcttt attcccccaa aagtccaaca accaaataga atcttcatgc tttattactc
    19561 ctccagtcac caaaatcctt tctaagtcaa ttggagagac ctttcgtttc atatcttcag
    19621 ctttcaaatc cttaacaact tctattgtgt tttttgcaac ttcatttcga taacaaagca
    19681 attcatcaat attgactaaa ttatcgtata tctcttttca ctttaaccat ttttttcttt
    19741 ataggtatct ccttatattt ttttaatatc aaatctccct tataatttaa tatatctaca
    19801 tatgaacata aatcttgaac ttcaattaca cctatatcat cattagttat accttttata
    19861 ttactaaatg caccaactat atctaaaact cttattttct tcttcttacc agcatttaaa
    19921 tataatttaa caacttcact ttctatattt agttttatat ctttactttt gtaagaattt
    19981 ttatcaactt tttttctctt tgatactttt tttaggattc cttttgaata ttcttcaaac
    20041 aaaactttgc catgtgtaat tctacttttt tctatatctt ttaattcatt tattttatat
    20101 cctatatacg tttctatatt ctctatatat ttttcatcct tttcagttac tatagtaatt
    20161 gcctttccac tatttccttt tctcccagtt ctacctattc tatgtatata gttttcttta
    20221 tcttgtggca catcataatt tattactaaa gaaatatcat caatatgtat tcctctggac
    20281 gctacatcac tactaactaa tatattaaat ttttggtttt taaaatcctt aattacaaaa
    20341 attcttcttt cttgtgataa atctgcatgt aactctctta ttaaaaaacc ctctttactc
    20401 atcttgttat ataatttaga tactttctct ttggtattac aaaatattat cacactcttt
    20461 ggattttcgc tgtatattaa ttcttttaaa aggatgtact tctctcttcc atttgccttg
    20521 attattttgt catctatttg tcttgttttt tgattagtat cgctctcatt ttcttctata
    20581 tttataacac tgtaatctag catatatttt tcacatatat attttatctc ttcatctatt
    20641 gtagctgaaa aaagacttac tattttctcc ttaggtaaat ttaataatat tttttccatt
    20701 tgttcaacaa aacctttatt aaacatttta tctgcctcat ctattactaa atactttaca
    20761 ttttccaact taatagaacc tctatttata tggtcaagta ttctgcctgg agtagctaca
    20821 acaatatgaa ccctttgttt caattcagct atttggtctt ttatagattg tttgccaaat
    20881 atagcactac atctcacctt ctttagtctc cctatatctg atatctcatc ttttacttgc
    20941 aaggctaatt ctcttgttgg aaccactata agagcttgaa tattattgta atctacattt
    21001 atattctcgc ataaaggtat tgcaaaactt gctgtctttc cacttcctgt ttttgaccta
    21061 actactaagt tttgaccttt taataatttt ggaataactt ctctttgtac tctagaaggt
    21121 atattatatc ctaaactctt caatgactta agtatctttt catttaactt aaatttttca
    21181 aatgtatatt tcatatatta ccaccttatt atatttatct tatctatata tttttctaca
    21241 ttatattatt ataatacaag gttctactat ttttatatta tttattagac tgaagataaa
    21301 agtatatact ctataaacct atagtttagt agcaaattaa ttaaattttt atatttaaat
    21361 acctccttaa atatactgat aaatcaatat tttcaaggag gtatctcttg tcaataatca
    21421 aactaatcta tcttttgaaa gtttaaatta tactaataca ttgtattttt atttatatct
    21481 aatattaaaa ggtattaatc aatacctaaa tcaccataat ctcacatatt tatatcttta
    21541 ttcaatatac cttctgtaat caatcgtgct atataatcat aacctgattc atttgctttg
    21601 ttacaatcat tcacattatc acagaaaaaa ctttcaatct gtattgcaac tggatttgta
    21661 tttctcaata tatataaatt ttctcttttt ttagctcctc tattttcaaa taattctcca
    21721 agtctattag atactctttt agcatattct aatccttcag tgccatagta aagcacttct
    21781 gtaccacatt gtgttccatc agctttattt aaatgtagct ctactaataa atcgtattta
    21841 ccactgttta cttttggaac tctatagaaa aattcttctc tttctgacat aaagtgcctt
    21901 tctggacaaa ttattacatc tactgtatta ccttgactta taagcatctc agctaacata
    21961 ggagctaatt ttttattata attgtattca tcaactatac catttacacc tgtactttta
    22021 ccacctttta gtatactatg acctactgtt atgcatattt tcatttactt ttttcacctt
    22081 cttttaagcc aatttttata taatagtaat ataattttaa agttattact aataataata
    22141 tattaattga agaaggtaaa agttctccgt gattatcatt tttttaacat atttgtctta
    22201 ttttatcata aatctatatt ttttaaatat tatactatac ttattattta taattgttta
    22261 attaatcatt tatatatatg ataaatatat caatatctat tttcctttaa ccaaatgcta
    22321 ttttaataag tttaagtttt agctttaaaa tacttaataa tacaataaaa gtgatgtatt
    22381 tacaaataat tcttctggat ttaattcatt tattgatgta tgttcaattt ttatatttat
    22441 atttttcata tcttcataaa taattccaac tggagaacaa atgaaacaag aaaatccaaa
    22501 agtttttgtt atttttttta ccttattatc accaaaactt atttgctttt tacaatcgtt
    22561 tatattttca ttgttatttt gtatatcatt tttataatac ttatcttttt taatgtcatt
    22621 agacttattt actgttttaa aattactcct atttttacaa ctatagtcta tttcaacttc
    22681 tatataccca aaaactatca agttacataa tttcaaatta ctacctttgt ttataagatt
    22741 tttcataggt ttaacatcaa atattttact atttacattt acttttaata tatctcttag
    22801 atttttagga gcttttaaag tattacttat attcaattgt ttgaatacaa ttttatttac
    22861 attagtataa actctattac tttctattga tattcttgtt gggaatttat ctagattaca
    22921 cttataataa tagtatatat tagcactatt ctttataatc ctatgtgcag aatttggtaa
    22981 aactacactc ttaaaagata ttattttttt acaacttgca tctattctcc ctatgtaaaa
    23041 accttgctgt gggtctaatc cacgttttat aatgccatta acttttaccg tattatttat
    23101 aaactctata tatttagaaa tataatcttt gaaaaatata tttccagttt tgtacctact
    23161 tgtatttgta acaataatag tgtaggtcaa aatctcaaat acagatgcac attttttatc
    23221 aacatatttt tctactttta taatacttcc atttgcatca tttgaaaata catcattaaa
    23281 agtagttttc gttattctca ttgcatccac ctttctataa tttaaagatt actctatctt
    23341 ggaactattt tcataactta taattgcagt aacactaata aataaattat catctaaaat
    23401 agatgaagta atatcttgta taagatactt taaattaatt ttatctttat tttctatctt
    23461 tctaggcatt tgtataaatg tactaaaggg tatttttaat ttaaatgtat tttttttact
    23521 agaatttcta tttcttttat tgcctgcata atctaatatt gcatcaatat ctatatcacc
    23581 aactaaaatc aacttctttc ctgtaagttg ttcatcttct aatgatatac attctatagt
    23641 atcaacaaga cttatattgt taatattcat attgtaaaca tatgtctcta atgaatttat
    23701 tacagttttt ttagaagcta atttaaaatt tttatatgta ttaaattgag ataaataaag
    23761 agtcttttct agtttttcac atctttcaag gttgtatatt accttattat tttcatacat
    23821 aatcgcatca atctccctgt atctatatat taatactatt cataaaaatt atttcaaatt
    23881 accatttttt attattcact atctagttgt tatttacttt taagaaagaa tcctttttta
    23941 cttcattgat ttcaatttac aagtaaaagt ctttctgaaa aaaaaatata tatatctcta
    24001 ttcacttttt tataagtaat tataattttt atagactaac ggtcagcctt aagggaaaag
    24061 gtgttcttta agtgcagaat ttagataaag taaataatcc ttttataaat aattccaagt
    24121 tttaacttaa ataatttgat ttacagtata aagtaaattt tgaaagataa tgatatcgat
    24181 ttaataatta caagtaaaag catgtcaaaa taaggctatg attttaaaaa tcatagcctt
    24241 atcaataaaa atttaatcaa agttacattt cactttgaat ttcactgtct attaattgaa
    24301 gattatactg ttgaattaat tctattagac gagctgcata tatactattg ccattttcat
    24361 ccttcgcagt actataacct gccttttcta atgctttagc ttgatatata tacgtttttg
    24421 catcaaatac accattgttt ttataccttt tatttgtggc taaaaatttt gcatggtctt
    24481 ttatagattc atttttatca tcatatattc taaatttatc atctataatt gtatctaaat
    24541 gttctttcgt ttctaaagtc actgacttgc ctttccaata agcgtcagcc tttataccaa
    24601 atagattgtt atattgtttt gccaaagttg acttgcccca gctagattct attatagctt
    24661 gtgcaatagt tatagatgga agtattttat aatccttata attttgtatt gcttctgttt
    24721 taatttcatc aataaacttc atatattttg tatcagactt caatctttct ggttttatac
    24781 cataatcctt aagattgtct atataattat caaccaatct tttttgtcta ttactaaatt
    24841 ctaactcatc caaaatatca cttaacttat ttattttatt atttttagaa aaattcttaa
    24901 caaataaatc tgatacttct tgtatctgtg aatccttaac gttttttaag ttatttttat
    24961 ttaacacagc aacaatggaa gctacatact tccagttaac ttgtgcatta tttttacttg
    25021 ctaaatcagc atattttata tacttactga catttatatc atcctttttt atctctctag
    25081 atgaatattc cttcactaat acacctatta aaattgatat tatcagtatt ataacaatga
    25141 attttttatt cttcataaat attgtcctcc cttatactat ctgctgaata taatatacct
    25201 tacttttgtg acatattctt tacgaaattt tgaaaatttt atgaagattt atgaaatata
    25261 tttaattaaa agcactacta ttgttataat atatagtttt ctatcacaca aaatagttaa
    25321 aataaataaa taattagata gcttatttta actatttata taataaaaat attattaaat
    25381 ctatttatca atttttatac atttttctaa tatatatttt ttcccatata caataatttt
    25441 tattggttta ccttttaaat tttctatttt aattaaatca ctacatacat ctacattaat
    25501 aatattatct ctataattta tattgaaaga ataatttttc cactcttttg gcaacttagg
    25561 gtcaaaagat agagtttcat tccatgttct catgcctgca aatccatgta caatggaaag
    25621 ccatgaacca gacattgaag ttatgtgcag tccatcattt gtatcattat tgtaattatc
    25681 taaatcaagc ctagctgttc ttgaatataa ttcataagcc ttttctaggt tatctatttc
    25741 tgaggcaata atagaataaa tactagaaga taaagaagat tcatgtacag tatatttttc
    25801 atagaagtca aaatttctct ttttttcttc tttagtaaat ctatttccaa aatagtaaat
    25861 tccctgtaat acatctgctt gttttataaa acacgaccta agaatcttat cccaagacca
    25921 attttgatta agaggaaaat tttcttttgg taaatccttt acttctacaa attctttatc
    25981 taaaaaatta tcattttgaa ttattatttg tagttcctca tcatacagca gatacatttt
    26041 atttgatatt attttccaaa actcaacttc ttccctagtc acattatttc tgtcaataca
    26101 ttcattacac tctttctcta atcgtaagat attttctaca gcataatcta aacaccattt
    26161 agctatataa ttagtatacc aattattatt gatattgttt tcatactcgt ttggacctgt
    26221 tactccatgt atcatataca aatctttttt tgtagacaaa tgaactctac ttgcccaaaa
    26281 tctagctatt tctattatta catctattcc tttttctttt atatatttat agtcacctgt
    26341 ataatttgta tagttatata ttgcatatac tatacttcca tttcggtgga tttcctcaaa
    26401 tgttatctcc cattcattat gacattctac tccatcaaat gttaccattg gatataaagc
    26461 acctttaaga cctagttttt ttgcattatc ctttgcctgt tctaactgat tgtacctata
    26521 ttctaaaaga tttctagtta cctccttatc tgctacacct agatatactg gaacacaaaa
    26581 cgcttctgta tcccaataag tagctcctcc atacttttca ccagtaaatc cttttggacc
    26641 tatatttaat cgcgaatctt ctccataata tgttgaaaat agttggaata aattatatct
    26701 tataccttgt tggctacttt tatctccatt tatttttaca tctgcacttt cccatctttt
    26761 attccataac tttatatgtt catttaatat atcatcataa cttttagata gttctctatt
    26821 taaaatgtct tcacattttc ttaatagctc actttcctta taatctctag ttgaggttat
    26881 agatacatat ttttctatac ttgcaacctc attttttaaa acatccattg ttatcgaatt
    26941 ttcacagtaa ttataatcac tctcatgaga tacagcttct acatcaccat ttgtcttaat
    27001 atacatcatt gaacccacag tgaatactgg tgtattaaat gcattctcct ttgttcgaga
    27061 aagaatactc cctttatatt ctaaagaact tgaagaaatt ccattccaaa acttttcctc
    27121 atagtttgaa tcttcatttt ttatatttga atctaaatat ggtacaaatt ttattttagt
    27181 attagaattt aatgatttta tactgtattt aattactgca agttccttaa cagatatact
    27241 taaaaaccta gtaaccctaa cttcaaattt tttatcattt atatttacaa taaacctacg
    27301 atttaaaata ccatttttca tatctaattc tctatgaaaa tcttctactt tacattttgc
    27361 taaatctaat tctacatcat ctatgaaaat tcttacacct atatagttga ctgaattagg
    27421 aattttacca aagtattctg gatacccatt tttccaccaa ccaacacgag ttttatctgg
    27481 aaaccatact ccagctatat aagacccctt gtgtgtatct ccactataat tttcctcata
    27541 gtttcctctc atacccatat atccattgcc tatactcatt atggactctg ctaatctatt
    27601 ttctttgatt tcaatcttat cttgtataat cttccactca tctacattac taaataaaca
    27661 tttcatataa ttctcctaac tatatactca ttatactttt cttgtatata ttcaaacttt
    27721 aattgatttg ttgaatctac aactaaattt gcttttttta agttttcata atttccaaca
    27781 ccaacagaaa acatattagc tgagtttata gcgtctatac ctgcacttgc atcctctatc
    27841 cctatgcaat tttgtggatt tacattaagc cctttcgctg acataagaaa tatctctgga
    27901 tgtggtttat tgtttttaca ttttcctgca tcagctataa aatcaaattt gtcacttata
    27961 cccaaatgat ttaatacatt tattgcattc ttacttgcag aagatagacc tatctttata
    28021 ttattagatt ttacatcaat taacaagctt tctatgcctg gcaaaatatc atttgaagtt
    28081 atctcatcta tcaatgatac atagtaatta tttttttctt cagccattct cactttttct
    28141 tcttctgaaa atgaatattt cttatttcca aattctaata tcctgtccaa tgactccatt
    28201 ctacttatgc cttttaaaga ttcattaaat tttgtgtcta tatctatccc aactttatga
    28261 gctagttttc tccaagccat atagtgataa tatgcagtat ctgtaattac accatctaaa
    28321 tcaaatataa atgcttcaat cattcttatt tcctctttct acaagaaatt tttagactaa
    28381 taatcaattt attttctatc ttgtatttaa gatattattt ataaaattat ctactctaac
    28441 tcagtcttta atttaaaatc tactttcaat tcactcactt gtagaccata ttttttatcg
    28501 tttataaaga cttgtttaaa ctgtataata atttaacatg aaatctaaaa taaatacttt
    28561 tttgtattat tttaaaataa tgaacttgta tttctctaac attagttctt cttttaaatc
    28621 aactatttct tcttcatata aatcttttac ttttttattt gctaaataat ctggtaacgt
    28681 caaaattata tttttagatg aattatttat tattatagaa atagattctt ttttcaatat
    28741 aatcgttcca ttttccttat ctgctaacac ccattcattt gatatagacc taagttcttt
    28801 attttcttta cgaagtctaa taatcttctt cataaaatca aatatatcct tattccattt
    28861 ttcttcattc cattccatac attttctttg accttcagat attgaactta attctccttc
    28921 cattccgatt tcatcaccat agtatataca aggacatcct gtttgtacaa acataaacat
    28981 atatgcaagt ttaaatctat caatatttcc attagcataa gtcaatattc ttgtagtatc
    29041 gtgacttcca agtaaattaa aaataacttc actaacttgt attggataac ttaccattac
    29101 atcatttatc atatacttaa attcttcggc gtttatctca tctgtacaaa agaatttttt
    29161 catggcttct gacatcaagt aattcataac tgaatcaaat tggtcaccca ttaaccatgg
    29221 taaactacca tgccaaattt ctcccaatat ataaatgtct ggttttactt ttttgacttc
    29281 ttctctaaac tttctccaaa aaacatggtc tacttcattt gaaacatcta atctccacgc
    29341 atctatatca aattcctgta tccagtactt gcctacatcc aataaatatt ttattacttc
    29401 tgaattttct gtattaagtt taggcatata tttttcacac ccaaaggttt cataaggtat
    29461 atttttttca tctatttgtt ctattggagt atcaatttta ctcatatctt ttatataaaa
    29521 ccagttttta tatcgagaat tttccttatt ttgaactaca tcttgccact gtttagaata
    29581 atatcctata tgattaaaaa cagcatccaa cattattttt atatttctct tatgcgcttc
    29641 ttctattagt ttttttaaag tctctttatc tcctaatgta gggtctacct ccatataatc
    29701 taccgtatca tatctatgat tggtttttcc aattgtaata gggcaaaagt aaattccgtt
    29761 tattcccaaa tcactcaaat aatctaagtg ttctatcaca ccttgtaaat ctccacctgt
    29821 aaaattatct ctggttggaa tagctcccca tttatctact ccttctgggt ttatagacgg
    29881 gtcaccattt gcaaatctat ctggaaatat ctgataccaa acagtatctt tcacccaatt
    29941 tggcacttta ggtatatcta tataatttaa atatggatat ccaaaaaaat tacctatttc
    30001 acataattcc ttttcatcat tttcatttcc caactctatt attctttttt ctgtaaataa
    30061 aagtttttcc tcttgacctt ctattataaa gccatatcgt gagcgtttat ttaaaggctt
    30121 acacttagct atccaatagt caaataattc agtttctact tctttactca ttactatatt
    30181 tgttccacca gaccaaccaa ctccagatgc atttaaattt cctcctcctg cacctccttt
    30241 atcccacaca tattggtctc ctattcgtaa aattactctt ttcgcttcat tttttttagt
    30301 tctaagtctt atatgtagtg tttctctatc ataaccatag gcataattgc tcattggtat
    30361 atgtaatata gcttcttttg ttaaactatt ttgaattgtt cctttgattt gattacttaa
    30421 agttatattt tctttatttt tattagacat tttaaaattt ccctccttta taataattta
    30481 tataatctaa agataggtta atatatttta ctattatatc acaataaaaa tatataatag
    30541 taaaatatat caacaataaa ctattcaatt taaaataagc tatactattt aaatttaatt
    30601 ctatttagtt attttagcaa aataaaaacc tctagtattt ttctagagga tacaatacta
    30661 taactattaa ctttttaact atctattaat ttacttcaaa acacactcta agctatattg
    30721 ttagatttaa ctttttctat atttacaaaa taggtttctc tttctttaaa taaagataca
    30781 tatttaaagt caagatactt aagcatatca taggccacat caaaatcttt gcatatatct
    30841 tctaccctat gagaatcaga acctacagta actataaatc ctccaagttc tttatatctt
    30901 tttagaatat cttcatttgg atgtatagca tttagtccat atctataacc agaagtattt
    30961 agctctatac cactcccatt attgattatt gactttaaga cctcatcaat aacatcttta
    31021 aagtcattgt atataactct attatctgaa tatattccat acctagatat ataatccaaa
    31081 tgaccatata tattgtaatc tccaaattca cgattagttt caagtatgct attaaaatat
    31141 ttataatacg catcttcttt gctaagacct ttaaaatacg cctttgatgg aacaggtaca
    31201 ttgtcaattg tatgagttga gcacaataca aaatcaaaag gattagaatt tattatttct
    31261 tctatttcat tttttagatg ttttctgtaa cccacttcaa cacctagtaa taactctatg
    31321 tcctttttat atttttcttt aaacttatta aatactggaa catagtcatt ataatttatg
    31381 caaaatcctc tatcttcatc atgttctaaa tggtctgtaa aagtcaaata tctaacacca
    31441 atacttatag ctttttttat tgtatcttcc atggaatcct ttccatcact tgaaaaatta
    31501 gtatgaatat gattatctat taaattcata ttatccctcc ctttatttta tctatcttaa
    31561 ttatacaaca agtttattgt atacatttgt taaaagaaaa ttataatggt taaattttat
    31621 ttacatactt ttatttttac aaaaaagaga agcacctatt tctgatttta gaaaatcatt
    31681 aaggatactt ctcctaatgt tacttttata tacttcaatt taatttttaa aagtcttaat
    31741 tttgtaatag agtttagctg ttatatgtcc aatcatataa aaagctgtat aaagtaaaac
    31801 atatatatat gcagaatcat ttaaaaatat atacattaat actatataag ttactaaaga
    31861 aacaataaga gataaaatcc aattagtctt atttaataca caaattatcc cttgtatcaa
    31921 aaaagaaatt ggaaaaatca aaaccattcc cacaacaaaa atacttttag attgaatatc
    31981 tggaatatta aatatatctg tagcaaaagt taaaaacatt agcaccatta tgactgtagg
    32041 aaatatgata ctaaaaatat tactcttttt cataataatc aatccttatt tattggatat
    32101 ttgataatct tcaattattt tgttgcctca atttctccca tcattcttac taaatcttgt
    32161 ccatattgac ctccagccca attattagat aaactttcaa ctgttttagc cttaccaaat
    32221 aaagattttt catgtctagg gtctactgga tttgaaagtg ggtaaccatc ttgtcctgca
    32281 tataagcata aatgttgaat ttgagctaat attccttctt cccaagtatc aaatcttgaa
    32341 tgggcattct tatcagtatc agaaccacct acagaagttt taagtccaca aggattctta
    32401 aatgatgcat ctaatactcc accaaaatta caaaaaccag tttcttttgc agattgagct
    32461 actgccaaaa caggattaac accttcctga acagcagtat catataatat tggtattaac
    32521 tctataaata aatcattagc ttttttagat tttgcccatt ttaagcacgc ttctaaacta
    32581 gctgttggct gacctaatat gcttgtctca tttttcatag gtgactcacc ttttgtagac
    32641 gaatcgccat tattttgtgc agcagaaaca cacttattat acacactgtc acttattgaa
    32701 cctaatctag taatcttatc tgctcctgct aatatagact tatcactact ttctgacacc
    32761 aatacaattg gattttcctt agcaattgta gaacctgtaa gtgcatttac taaatcatat
    32821 ccatcactca aataaaattc tgatgcatct ggataaaact cttttataac tttcttattt
    32881 gtatcatatc tatctgaacc accaagtctt ttagcattag ttttattaac taaactagtg
    32941 ctcattgata tactcccacc tactgcatat gtttttacat tgcttgttga aaaaggcaca
    33001 ctctttccat ctgtaagtac tataggagct atatctctta ctgatacggg tgcagcactt
    33061 attgtatctg cttctccttt atatgcatta gttaatatca cttcatcaac ttttttgata
    33121 ccatttacct ctttagcaac actataacta gtctttattc tgtcatctcc acccaatcta
    33181 gttacctgtg cccctgtgtt ttttagttca gtttctactg acttacttat tgataattct
    33241 ttacctatta tgtatatctt tttaacattt tttaatcttt gttttgtttc atttggtatc
    33301 ttattttttt gtgcaagcaa aattggagca tcaacagcac ctgcaagacc acttgctgaa
    33361 agaccatctg ctatagaatt atccatattt accataattg ctgtatcata actcttttta
    33421 tctgctatta atccagccgt ctcatatata ttgttacctt taattgtatc tgatgttaat
    33481 gcatctacat taggtacatt tattagaaat atagctaatg ttaaacctaa tgctaaaact
    33541 cttctaattg ttctcatatt cttccccctt gatatatcaa gtttaattca aaaatgacta
    33601 taacatagta ttacatctac gctatagtca tttattataa tttaataatt atatatttgt
    33661 tattatatca taaattacaa aaagtttcca taatattgta tagattaaag gaaaaattta
    33721 cattttagtt acaatttact agatttttgt tctaatgtac ctatatttag taacaatttg
    33781 taataaaaat cgatatctat attacatatt taattcactg ttttacaaaa tgaaatttta
    33841 ttgtatacaa taaatagatt atatgtttac ataatctata ttatttttaa gtaaatcttt
    33901 tatcgatatt attaagaata tatattacat caagtttatt acgtgcaaac cactatcaac
    33961 ttaaatacct aaggtaacac ttgaagtagt agattcttgg taacaataat tattgctatc
    34021 attatctttt tttaattaag tatttactat ttatatatca atctactttt cacttttaat
    34081 ttaattctaa tattttttta tatgttttat gatatataat gtgatatatt ctaattaatg
    34141 atattttcaa gtaaaaaaat gttactctat tataaggtga tagtatactt gaaaataaat
    34201 aaaacactta actttattta aggagattta tgatgagtaa caaacaaggt tctaacatca
    34261 aagatgtaaa aattaaaaat aagttaataa tattgaaatt actttctaca aatacaccta
    34321 tgtccagagt tgatttagct aagtctacag gacttactaa aatgacttta tcaaatctag
    34381 ttactgaact tattaatgaa aatatgatta gggaaataga gtcatcaact ttaaatgcac
    34441 attcatcagg tcgtaaacct attttattag atatttctaa tatatcgcct tgtatttgtg
    34501 gaatcctaat aagaagaaat atatgtaagg taattatttc agatttaaaa ggtaatattg
    34561 taaaacaact ttctgaaact taccctaaat ttctatcaaa tgatgtttta aaagatataa
    34621 taaaaagaat gtttactaaa ataatgaaaa tcaacaagag agatataatt ggaattggta
    34681 tttcttcaat aggtccagta gatgatataa atggtaccat actaaatccc ccagattttg
    34741 gaaatgtatc aaacctaaat atagtagatt ttataaaaga aatgtctgat ttgccaatat
    34801 ttttgataaa tgatgctaat tcgggtgcat tagcagaaaa aatgtatggt ttaggtaaaa
    34861 atatatcaaa ttatatatac ctacacataa tgaatggtat tggagctggt ctagttctag
    34921 aaaataaact tcatactggc aacctaggac aaagtggaga aataggacat acatcaatta
    34981 attttagtgg accattgtgc agttgtggta acaatggatg tttagattat tatacaagtg
    35041 tcagtaatct aattaaaaga attgaatcat tgagccatat ttatcctaac tccccattaa
    35101 ttaactgtaa agatttttct ttagtcaagt taatagatga ggcaaataat aaagatagtc
    35161 ttgccatgtt tttgcttgat gaattttgta catacgtatc atatgcatta gttaatacac
    35221 ttacattgat tgattgtagt tctataataa taggctatga ctttaatata cctggtacat
    35281 ttatagaaaa tacattatta aaaaaattga ctgcttcagc aagtttttct aaatataaaa
    35341 aaataagtgt aaggcattca aattttggag caaatgcacc attaattggc tctatagcca
    35401 ttgtaagtta taattttttt aatggtagta taaattttta ttaaatatgt aaaatattta
    35461 aacactttta ttgaatattt ttccatttaa tattattata ttttataaga gcatttaaaa
    35521 tttggaggta taaaaatgat ttcagctaaa atggaaaaat tacttaatga gcagattaac
    35581 catgaattat attctgcgta tctttatctt tctatgtctt cttaccttga agcagaagga
    35641 cttaaaggat tttctaattg gttttatgtt caatataaag aagagacaga ccacgctatg
    35701 tttttttaca aatacttaca taatgtagga ggaaaggttc agcttgatgc tatcccaatg
    35761 ccagattcag actttacttc tgcaatggat attcttgagc gtacgttagc tcatgaaaaa
    35821 aaggttacag ctttaataaa caacttagca gctgtagcaa actctgaatc agactttaga
    35881 acttctcaat tcttactttg gtttatatct gaacaagcag aagaagaaac aaattgtgaa
    35941 gacaatataa aacgtgttaa acttgctgga gagggtgggc tattctttgt tgaccaagaa
    36001 tttgctaatc gtacatatgc agctccaaca aacccacctg tgacaatata acgtttaaaa
    36061 atttttaata taataaagaa ggtgttttaa tgaaaatcaa gattgattct tatcattaaa
    36121 acacctttta ttattcgttt tatacgtagt attttattgt ttatacagtg ctatttcaca
    36181 atgaaactta aaactatact ttcattttaa aatattttta aattaaattt ggggtgactg
    36241 aaaatcacac tatctaactt acaaaatgtg attttcagtt ggtgctttaa ttatggttga
    36301 ataccaaaac ttataaacta tttatattta actattaagg catctactgc acatacgcct
    36361 tttcctttag gatatacaaa cactggattt aagtctattt ccttgacttc atccttattt
    36421 tcataagcat attttcctaa cttgaccatc atatctgcaa gagcatcaat atcacatggc
    36481 tcacttcctc tatagccatt taaaagctta aatgatttta gttttttaag catcataata
    36541 gcttcatcat gatttatagg taatggatat aatgtagtat ctttaaatac ctctacaaat
    36601 actcctccta aaccgactaa taacattggt ccaaattggt catcattagt tataccaata
    36661 ataatttcta taccactttc taccatttct tgaactaata taccatcaat attagcatct
    36721 ggttttgctt tttttacatt agttaaaatc tcattatatg catctactgc ctcatctcta
    36781 ttcttaattc ctattttaac tcctccaaca tcagatttgt gcaatatttc agaagaattt
    36841 atttttaaaa caacaggata cttaatatat tttaagattt catctaattc atcaatactt
    36901 tttgcattac cttgtcctgg aactggtact ccatattttt tcatctcaat cttactatct
    36961 aattctgata atgctactac attatttgac tctcttttct taacagcttt aaattctaaa
    37021 gttctttttg aataatcata atctataaac ttagccattt tattaaaaca agtaaatgta
    37081 gttccaactg atgacattat aggcacttga ttatcttcta atctagtcct ataatctaaa
    37141 tatctatttc cttcaagtgt aggaactgca aacacaggtt ttttcaatcc ttgttctcta
    37201 gcttcactta cagctttaca aagggtttca tgcatttcat tttcttcatc tcttatattt
    37261 gcacctacca tagtaaatcc aacactatca tccttttcaa aggtatgtaa taaacctact
    37321 atcatatcct tttcatggaa taatgctgtt gtagcatcta gtggattttt aggtgttgca
    37381 aatcctggaa gatatttttc catttcttct ttagtttcag aagatatttc tgcaagttct
    37441 acaccatttt cttctgctaa atctgcacaa atagtatttt ctcctcctga gaagtttatt
    37501 gctgcaaaat tagaatttgc aggtaaatta ccatctaaaa cactaaaagc ttgtgctaaa
    37561 cacataaact gttctaaatt ttccacagaa attactccat atttttcaaa aatactttca
    37621 aaagctttac tcgaaccagc taaatttcct gtatgagaag cagcagaaat agcacctttt
    37681 tctgaccttc ctgatttcaa tattattact ggctttcttt tttgtgctgc cctttttaaa
    37741 gcatctataa atttttgagg attttttaaa ccttcaaggt aaattgctat tactgatgca
    37801 taatcatcat ctacaaggaa atctactgca tcttctagtg taactatatt tccatttcct
    37861 gttgaaaaac cataagatat attaaaataa tcacttgaaa caatttcagc agttataaat
    37921 ccactttgac ctacaattcc tataccaggt tttttagtat ctaaatccca tttagaacct
    37981 cctgcccata gattaacctt atctatacaa tttagcaacc ccatgcagtt tggacccaat
    38041 attttcatat cgtatgtatt tgatatttct ataagttgat tttctaaatc tgtaccttcc
    38101 tctgtacctt cctctgcaaa tccactagca tataccactg ctgcctttgt tcctaactct
    38161 ccagaatctt ttaatacaga tggaactaca tttctaggcg tacatactac tacacagtct
    38221 actacttctg gaacttcttg tatcgtctta taacattttc tcccaaacaa ttcttctctt
    38281 ttgggattaa cataatatac tctatctgtt tccttacttt ttactatact aagtgctgca
    38341 cttcttccaa aaccaagttt atcagtcact cctactatag caatactttt tggtctaata
    38401 agtttttcca aactcataaa ttcacgctcc tatatgttta tagttgtgat tttaagtgaa
    38461 ttttatctca agatgaagtt tctcttggca ctaaagatta ttaaatcttg agataaatca
    38521 cttaagataa gggtaattta tcaaatggcg ttattatagc cttccatgaa tgctgcttca
    38581 ttttgaacat taaactttcc ttttcctttt tcctcaaaga aatcacacat agtttttaaa
    38641 gctaattctt tttcaaataa cccagtttta gcaattaatg ctccaagtac tactatattt
    38701 gctgcttttg gattgtttac tttttgagct aattcctgac aatttatttt aactacactt
    38761 atatcttctc ttaatgttac attctcatct attgcagatg tattaataac tactgttcca
    38821 ccttctttaa ctaaatttat ataatctaaa gatggtttat tcattgctac taaaacatct
    38881 atttcttcca tatcaggccc acctatactc tcatcagaaa actttactac actataggct
    38941 tttccacctc tcatagttgg accatatgca ggcatccaag ttacttgttt ccccaattca
    39001 agtgctgact cagctacaat cagaccaaga gttaaaactc cttgaccacc aaaccctgca
    39061 aatactagct ctttttccat aactattcac catccctttg cttaaattcg ccaagagcat
    39121 aatatggcac tatttcttct tccatccatt tgatagattt ctctagtgat acaccccagt
    39181 tagttgggca aggtgctaat atttcaacta aggaatatcc ctctccattt aattgagctt
    39241 ctattgcatt ttttatatat ttttttagat taattatttc ctttgggcta tgaactgaac
    39301 ctcttgctac atatgctaca tcaaagaagt ttgccaccat ctcaggaact ttcagtggac
    39361 taccagtagt ctttatatct cttccattta cacttgttgt tgtgacttga cccggcatag
    39421 tggtccatga catttgtcca ccagtcattg caaaattatt attgtttatt acaaatacag
    39481 tcacattatg atttctatat gctgtattta aagtttcaga aagacctata acataagcat
    39541 ctccatcacc ttggtaagtc attactaatg tatctggaag tgctacttta actcctatag
    39601 ctgtagatga agctctacca tgtgcagttt gcatcttatc accattccag ctaaaattca
    39661 tattgcagac acaaccaact cctaatgtta atacatgatt tttttcatat ccatgttctt
    39721 ctattacctc tgctattata cgatttacta ttccatgacc acaacctggg caaaacttgt
    39781 ttggtattga aacaacgcta ggtgttaatt tttctgtagc catattagaa cacctccttg
    39841 atttcgccag attttatttt gctataagtg tccattactt gcaatgtttt tggtatatga
    39901 tttccactaa ataaaccata cacaggtaca ttttctttac atactttctt tgaggctaaa
    39961 gcaacatctt ctactaattg acctgtgtct gttgattcta ctgagataaa tccttttaca
    40021 tctttactta ccttttcaaa tgctttatat gggaaaggcc atactgttat tggtcttatt
    40081 attccaactt tttcaccttg ttctctaagt cttctaacag cattcatagt tgttcttgat
    40141 ggtataccaa acgctacaaa cacatattct gcatcttcta cttgataatc ttcccattgt
    40201 tgttcatttt cagttattct catacgtttc tccatacaaa aagttggctt ttggttatct
    40261 gcaacctttg catggtctct attacttcca tcataagtcc aacctaaatc tccttctctc
    40321 ttcttaaact ctggaaattc tactggttcc atcatttgac ctaaagctgc ttctgtcaaa
    40381 atttcgacag gcactctgta cttctccgct acatcaaatg agttatacat caaatctaca
    40441 gtctcttgaa tactgtttgg tgcataaact atcaaatgat aatctccatt tcctccccct
    40501 ctagtatctc ttagatagtc tgtctgtgct gagtccaatg agccaagacc aggcccccat
    40561 ctctgtacat ttacgattac acaagggtaa tgatttgcac ataagtaaga aatcccttct
    40621 tgttttaaag atattcctgg tcctgaagaa ctcgtcatag acctcattcc acttgcatat
    40681 gctccatata ccatatatat agcagccatc tcattttcgg actgtataaa agttcttcca
    40741 agttcagaca ttctatgtga taaaaattcc attacctctg tttgtggtgt aattggatat
    40801 cctgcataaa attcacaacc tcctcttagg gctgcttctg caatggcgtg gttccccttc
    40861 cacatctttt tcatattatc ccaactcctt ctaagcctct tgacatgctt cttctttaat
    40921 tatttcaaaa acattatctg gacacacctg atagcaaatt ccacaaagta tgcatttact
    40981 ctcatcaact tctacatgag cataaccttc tttatttatc ttacttgata tttttaatgc
    41041 tcctttttta caagatataa cgcagtattc acaactttta catctctctg cgtttatttt
    41101 taagttcata tctacacccc gtttcataat atttctatct attatccaat acttggctct
    41161 cctttaatat ttaatatttc ctttaaaact gctacaaatt tcctattttc ttccatagtt
    41221 ccaatactta cacgtgcatg tgtagcactt ggtcttatta caaaccctct ttttttcaag
    41281 tcttcaaata acttaggtat atctgttttc atatctacat aaaggaaatt tgattgtgaa
    41341 tctacaacat caaatcccat ttttgtaaac tcttctttaa agtaagccat tccaactcta
    41401 tttaattcat atgtttcttt tataaactct tcatcatcaa gtgctgctgt tgctccactt
    41461 atagcaacac gatttgcaga gaaaggctct tttactgtaa ataaagttct tattatctca
    41521 tctttagcaa taccatatcc tactctaagc cctgcaagac catatatttt agagaaagtt
    41581 cttgttacta taacgttata attttccctt acatacttaa gtccatccac ataattagga
    41641 actgttaaaa attctatata tgcttcatcc aatatagtta taatattttc agggactttc
    41701 tttaaaaatt cttctaactc tttatcatcc acaattgttc ctgttggatt attagggtta
    41761 catacacata ttaattttgt tttatctgta atcttatcta atataccttg taaatcatac
    41821 ttataatctt ctgttaaagg tacttctact ggtatccctt cattttttat agttgcagtt
    41881 ctatatgatg gaaatgttgg gttacaatat ataacttcat ctcctctatt tataaaagcc
    41941 tcacctatta gagtgataac gttatctgca ccattagcaa caataaaatt ttctggtttt
    42001 aaatctagct tttcagctaa ttttcttcta agttcgttag cttctggctc tggataaagt
    42061 tgaccttgtt ttaacatttc aatcatagct tctattgctt ttggtgatgg tcctagagga
    42121 ttttcatttg aagctaattt tattatttct tttacaccat atttttcctt aactttttct
    42181 attggttcac ctggaacata ttgatgtaat tcttttacgg tatttctcaa taatttttct
    42241 atcattttta aaagccccct ctgtatatgt attttcattt atataaacgc tattgcgtct
    42301 acttacttta atagcttaaa ttaaccatct actcaacttt cttttaacta agcatttctt
    42361 tgatttaact cttctcttat ttggtttaat ttcttgtcac ttactggata tagtattgct
    42421 ataacaacag ctattattaa aggtattgcc atagaataag aaatcatatt tataaaacct
    42481 gatttaactg cttctgtgac taccatatca gcttgatatc ccattgagtt cattaaaact
    42541 gttcttatag tagttgcaag taatactcct acttgtattg caactggata tagtgacata
    42601 cttaagccag gtaagtcttt tcctgatttc caatgcccat aatcacaaga accggcaaag
    42661 aaattaagat ttattattga tactacattt gaaaaacctc caccaattgc caacaacata
    42721 gttactccta aagtagtttt cccccaagga gtccatgcaa ccaataagtg gcatagtgca
    42781 catatagaaa aagaaattat tgaagctagt ttttgctctc tacagatttt tataaacact
    42841 ggtgcgaaaa acatgaatgc tacacttacc aaaggcatga ttgataaaaa tactgctaat
    42901 ccacgttcat cttctattac atatttaaaa taatatggag ccaaaggtcc taatcctata
    42961 gatcttatac atataagaaa ttctaaacaa aatgccatca taagtggtac attagtgaat
    43021 aaagcttttg ccatttcact tgctttaatc tttggcttct cttctttttg aagaacttct
    43081 tcttctgctc cagattttat tatctctgtt gaagaaaacc aaaatactaa aaggcatcct
    43141 gctgcaaaga ataagtaggt acctaaataa cccattcctt ctccacctaa tacatctgca
    43201 aataaaacta ataataacgg atatgaaaaa cctactaaga atttaccaac ttctttaaat
    43261 atattcgtag tagttgagcc tataactcgg tctctaggtg tcttagcaac tacagggtaa
    43321 atagcataaa ttatagtgaa aactacttgc tgagcacaag atgctattaa taataaagct
    43381 gtcatcaaag gtaaaagtaa tgctggactt attttcatta atggaactat gaaaaatatt
    43441 ccatatgtta atgctaacgc tgctggtgca tatctaagcc atggataata ctttcctttt
    43501 ttgaatggtt gtttgtcaaa tactgtacct gttataggag ctacagcaat ttttatgaaa
    43561 gattgtataa tttgcatacc acctgccata attacaccaa gttttgccac atcagttaaa
    43621 aaaatatttg taacagttga tacaaagtaa cctataaaat tgtacgctaa atttatagaa
    43681 cttgaatatg tgtatattgc tcttttctta gggtctctca tatcatctct aaaatttgat
    43741 ataagatttc cttttggttt gctttcttga ctactcacat ctacccctcc cttataaaat
    43801 ttacttactt tttattattc acgtataata atatatattt tgaatcttaa atatctcatt
    43861 cttgattcag gagtatattt ttatatgtgt ataattttaa tttaattata ttctcaaaat
    43921 tcataattgt caaacacttt ttaagtttta ttttttacta ctaaaattaa ctaaaaagct
    43981 atttagactt aatttaggtt tgttttttat tattttcttg ttttgaaatg tcattgaaat
    44041 gcgtatttta aattatttta tcatatttct agtgatttta attttttata attttattca
    44101 agaataattt attcaagata tttctatact acatccagat attaaaaata tctttttctt
    44161 atttaaattc cttttatttt tatacatgta ctattgattt aaaaataaaa gcgtgttaca
    44221 attacaaaaa agaatctatg agataaatat caatatgcaa aaatttcaga attttcaatt
    44281 ttctattttt acgtaatttc catctaatat cataaattat tatagctata ttaatctaaa
    44341 accaataaaa caaatatcgt actactttta tataaaatat attttaaata tattttgtat
    44401 attttattaa aatattttat aaaggagaaa agactatgtt acttttaatt gttacgtgtg
    44461 caatatacat atggattggt ttttattttt caaaaaaagt taaaacagca tccgatttcc
    44521 taatcgcagg tagaaatctg cctttgccta tactagctgc aaccattgct gctacatctt
    44581 ttggaggagg gtgcttagtt ggtggagttc aatggggagc tgaaagagga ctttgggttg
    44641 gaatgtattc tactataggt gcagctttag cttgctttat aaatgcattt ttcgcaggaa
    44701 aattaagaag cttatcttgt gatataacac ctgctgatta tattgaaact agatatggtc
    44761 atagtgtatt tttaagagct tatcaaagtc ttgtaacacc aatatctata ctagctatta
    44821 tgggtagtca attgatttct tttggttctg tatctactgc atttggtatt ccttatgata
    44881 tagctgtaat tataggagtt atagttatca ttatatatac ttatacatca ggaatgtggg
    44941 gagttgcagt atcagcattt attcaacttg ctatttgtat agtgtttttg ccaatagtag
    45001 cttatataag cttaaaatta gttggcgaca accctttact tatgttacaa tcaatggtaa
    45061 aagaaccatt ctttccagga aataaatcga ttagtgattt tatgtataca gttttaccat
    45121 tagttttagg ctcaatattt tcttatgaaa gttttttaag atatcaatgt gctgataatg
    45181 ctaaaaatgc agttaaaagt agtgtaattg caggatttat actattattt cttgctttcc
    45241 ctataggaat aattggagct attggtggta gtttatttcc aaatatatca tctgttcagg
    45301 ttttacctca aatgatatcg actactttgc cacctttaat atctgtatta ttcttagctg
    45361 ctatattggc tgccataatg tctactgccg attcattaat gacctcaatg agtgctatta
    45421 tatcaagaga tatttataac aaattattac atcctaatat agaatttaat gatttaaaaa
    45481 atactctaaa gtattctcaa attgcatcag ctttgacagc tataataggt gcaatcatag
    45541 cacttaaatt tgatagttta ttagaattat tgttttggcc agctccatta tgtacaggtg
    45601 taatatttgc accatttgta attggtcttt gctggaaagg tgctacaaaa aaaggagcta
    45661 tctatgctgt tatagtaagt gttatattgg cattattaaa tatgattggt attcttactg
    45721 tatttgacag aattttggtt cctataatag gtggttctat tacaatattt atagttagca
    45781 aattttcaaa aaaagattga aaagagaggt atacacagta aatatgtcaa aatcagaaaa
    45841 gggcaaagaa acaaaagcta gtattataag tgctgctaga gaattatttt ttgagcaagg
    45901 ttatcataag acaacaacta ggcaaattgc agaaagagct aatataaatt taggtctaat
    45961 aagctattac ttctcaagta aaagcgaaat aggagctata atatatgaag atataagaaa
    46021 tgaaatgcag tctttaatct ataactacca tgaagaaggt actatgatga tgttctttct
    46081 tgctatgaat gctgaccttt gtttccttct aaaaaacgaa gcatatagaa atttttattt
    46141 ggatatagct tatgaagaag ctattttaag ctatcaaaga agtgtaaccg aaatggtcat
    46201 gaaaagatat gttaatgata aagaatctat agaacatgat gaattaatac tttcttgtat
    46261 tggtatactt gctatgaaac ctgaaataat tagagtttat acttcacaca agtacgatat
    46321 atcttctcaa gctgtaataa agcattttac aaaacagttt cttattaatc ttggtcatag
    46381 tacggattta tgcgaagaga ttttagagga attgagtaaa tactatatag atatagttga
    46441 caattttact ccaataataa ctcgtgtaag aacttaataa aattttatta aattcttaga
    46501 gactgaaatt ttaaaatcat aatttgattt taggatttca gtctttatca tatttaaaga
    46561 tattttatag tatacctgtt aataccataa gtgtactatc caattatatc tataaatttc
    46621 tttatccata aattccaaag acgccattca tgccccccta cacactcttc atattcaaca
    46681 tctacaccca gagaatctgc atatttttta aacatctgat tatttttata taaaaaatct
    46741 ctatcaccac aaaattgata aattttagga atatcttcat ttctttcttt aaggttctta
    46801 agtatgtaaa gtaaatcatt ctcactattt ctagcgtctt ctggtctacc aaaatcgtga
    46861 aatagaatgt ctgcattggt ttctatttgt tgattttcta actcatgaat catatcaggc
    46921 gaaccagaaa aacttgcaac tgccgaaaac tcttttggtt ttgaaagcgc taattttaaa
    46981 gctccatatc cccccataga cataccagct acaaagttat cttccctttt atctgataaa
    47041 ggaaatatag ctctaacaaa tttaggtaat tcctcgctta caaaagtaaa atattggtgt
    47101 ccatacgcca tatcagtata aaaactagtt tctactcttg gtattactag ggctatattt
    47161 ttatttttag cataatgctc tacattagta tatctaatcc atgagctttg gtcatcttct
    47221 aaaccatgaa ataagtataa tgttttaaat ttaccttcaa aatcatatat ctctttacca
    47281 ctaaaaagtt cttttccatt tgctacaggc aaaattacaa ttatatcagt ttctcctcca
    47341 gtatatttag atttaaattt acattctata aatgccacaa aaaacacctc ttctataata
    47401 attttatatc taaaaagcac caagagttta actttctcag tgctttaaat attgcttaat
    47461 ttatatattt ttaattatac ataatatttt ttagctacat ataatcggaa gtatctgtcg
    47521 gtaatccctt tttgtccaat ttactttcaa caatatcctt aattatagaa taaattactg
    47581 caaacattgg aactcctata accattccaa taaaacctaa taattttcct gcaaccaata
    47641 aggagaacaa aatccaaaat gcagatatac caattgaatc tccaagtatt ttaggtccta
    47701 taatatttcc atctatctgt tgtattatta atataatcaa caataaccag aaagctttta
    47761 taggagatac aaacatcact attagcgtag atggtatagc tccaaataaa ggtccaaaga
    47821 aagggataat atttgtaata ccaattataa ctgatattaa taatgtatat ggcattttaa
    47881 ctaacgtaag tattacaaat gttagaatac ctataatgaa tgaatctaat attttgccac
    47941 ctaaaaatct tccaaatgta tagttacttc tatgagttaa ttctaaaatt ctggcagctt
    48001 gtttttctgt aaatactgca taagttattt ttttgcttaa tccatagaat ttttctttat
    48061 caattaataa gtacactgaa acaattaatc ctagtacaat attccatata ctagaggcta
    48121 cattctttaa catacttcct aatattggga ttaaatctgt tacaaacttt ataatatatg
    48181 taacaaattc attccactta tcaactgcca aattaaagta ttgctcatca agatttaaat
    48241 cagtcattaa tttatcaata agcttagttg cattgtttac atacataggt atatcatttg
    48301 ctagtccaac aatactctca attacttgtg gcaatacgaa atgcataaat aagtaaagta
    48361 tcaatgtagc tgttgcatat gttaataaaa gacccaagcc tctttttgaa ctttgtttta
    48421 gtttttttaa tttctcactt tttataaaaa ttctgtcttc ataaaacttt aaaataaaat
    48481 taagaagata tgcaatagca aatcctatta taaaaggttg taatgttgta aatatagcat
    48541 ctaaattatt agaaaataca tcaatttgtg atgatactag ataaaatata atactactag
    48601 cagctattat aaatgtataa attgctattg ttgtgtattt tgtattccaa ttaattttca
    48661 ttttttcctc cttatttcta tataattaaa ttttatcaca aaatcaccac ttttataaca
    48721 aatataccaa attaaaaata aaatatattc agaatacaaa tactcaattt tatttatatt
    48781 tattattcta caatataacc tacattatct ttttaagaac cttaattaat ttattatcgt
    48841 atggagcata tctaataggg acatccaaat ttaatttttt taacacactc ttatagtgag
    48901 taaatgtatc gaatccagct tttccatgat atgagcccat accactttca ccaataccac
    48961 caaaaggtaa gtaattcgta gctatatgag taactgtatc attgatacaa catccgcctg
    49021 ctggaatcat ttctaaaagt ttattttcta ggtacttatt ttttgtaaaa atataaagtg
    49081 ccaatggatt tggcatttgt ataatttttt gtattacttc atctaaatct ttatatgtta
    49141 aaatgggaaa tataggccca aatatttctt ccttcattat attatcatcc caattaacat
    49201 tgtctactat agttggctct atctttaatg tctccttatt ataatttcca ccatagacta
    49261 tcttatcttt atcaataaga gataatatcc gattaaaatg tctctgattt atgattttag
    49321 gaaattgctc attattacat gcattatccc caaaatactt attagtataa tattttattt
    49381 tctctattaa ctcatttttt ctatcttcat gcactaaaac ataatctgga gccacgcagg
    49441 tttgtcctgc atttattagt tttccccaag taagtctttt ggcagtaagg tctatattag
    49501 catcttttaa aatgatacaa ggactttttc ctccaagttc taaagtaata ggagttaaat
    49561 gctctgatgc tgatttcatg attattttac caacattagt acttcctgta aaaaatatat
    49621 aatcaaatct ttccaatagt aacttttcac tttcttcttg acttccttga acaactgcaa
    49681 catattcttt tgaaaaagta tcttcaacta tgtctttaac tacactagat gttgacacag
    49741 aatattcaga aggttttaat ataacacaat tccctgctgc taatgaccaa actaatggta
    49801 taagtgctaa ttgaaatgga taattccaag gtgacattat gagagtaaca ccataaggtt
    49861 gtggaacaac tttggatata gatggaaaat taaccatact tgatttaact ctttttattt
    49921 tgctccattt tcttatattc tttatagcaa agtttaattc actataaaac atgcctacct
    49981 ctgtcataaa accttcaaaa tccgatttat ttaaatcttt ctttagtgct aaaagtattt
    50041 tttcttcatt ttgtgataca actaacttaa gtttcttcaa ctgttctata cgaaacgata
    50101 tatctcttgt tttacctgta ttataatatt ttctttgcat tttaactaac tcttttatat
    50161 ccattgttaa aaatcctttc tttaagaatt atactaatac atttttagaa aaagatttaa
    50221 atttatccta acattatttt ttatatcata ataaattcat ctcaatataa cataaatctt
    50281 ccaataaaaa aagcccctta ttaagtataa gtaagcttat aaaatagttt attttaaaat
    50341 aaacattttt taaaattata accttattga aacaattaaa ttatccatct ttaataatat
    50401 tattttataa ataatgtcta gtaagtttct atgaaatcta actttagata ataaataatt
    50461 acaaatatat acctcttaaa gttctcttat ataatcaagt attggagtta aattttcttt
    50521 attacaccaa tcaaaggctt ctccagacaa taaaggtgct tctgctggtt taagaccttg
    50581 acgttctttt ttagctatag attttttaaa aaaactacaa agagtataat ctataaaaga
    50641 catttttgtc tcatcccatg ccccacgaca ataaaagcaa ggaattttgt ttaattcatc
    50701 cttaaaattt acttctctta atttctttat tacttcatca tcatatggag cagctcctac
    50761 gccaaatact actaaatttt ttttctgcaa ttctttataa ttttttttta agaaagaaat
    50821 tcccgctata ttacaagcat aaagacctcc tcctaaaata atcgtatcat acttcataac
    50881 cttttctata ttaatattct tagtttcaaa aatatcacaa gacaataatt ctgaaagcca
    50941 tttagcatat ttttttgttg aaccatactt actcttataa atcactacta catttttcat
    51001 ataaattatc cccctctctg atgtcataca aagttgattt gatacttgct tattctccaa
    51061 cgtcattatt ttctacataa tttttcacat tttcaattcc tatacttaat ttcataattc
    51121 caaaaaataa ctgttccact tctttctctg taaaatcttc aaataaaagc tgtaatactt
    51181 tttcctgcat acctcttact tttttagaat actcttgcac tctatctttt gtcacaagac
    51241 agatagctct tgaatctttt tcattctgct caatttgtat aaaacctttc ttttctaaag
    51301 caatagctag tttttttaca ttttgtcttg agcaccccat taattctcca agtttggtta
    51361 atgtaagagt ttcatctaca gagtccgcaa tagctaaaag taaccattgt ttcattgtta
    51421 attctgggtc taatttatca catgtagttt gaagtttatt ttgcattaca aatatacttg
    51481 caaatatagc ttgttttcta ctatcatcat ctaccatata ttttttcatt aaactttctg
    51541 catccattat tatacacttc tctttatctt agattttaat tttttgataa ttttaaatag
    51601 taactaatta ctttatatta tagtaattag ttactattat gtcaataaat ttatttttat
    51661 tataaacggc aaaacggcat agcattatgg ctataccgtt tcataaaatc atatttacaa
    51721 tcaatttata ataaattata aattaaatat attttaggct ataataagtt tttcaaaatt
    51781 tactctaaag catacttttt atacttatta tattctatat tgctacattc tacatctaaa
    51841 atagctccat cttctctgta ttctgtattt ataatgctcg tattttcgtt aaaataagaa
    51901 actacatgac ctttatcata tggaattaag aacttacatc ttacataatc tttaaatatg
    51961 ctatcacaaa tacattttat cattttatct atatttatat cttttttaac tgaaacaaat
    52021 ataccttctt tatcgatact atctaaaatt ctatccaata cttctacatc tattaaatca
    52081 attttattat acacatgtat cataggtata ccatctgcac ctatttgttt taaagtatct
    52141 tctgtcactt taatatgact tttatagctt ggatttgaaa tatcaataac atgtaacagt
    52201 acgtctgcct cacatgcttc ttctaatgtt gacctaaatg cttttactaa gtcatgtggt
    52261 agattactta caaaaccaac agtatcagat aataaaaatt ctttgttatt tgcaagaact
    52321 atatttctga cagaagtttc aagtgtagca aataacatgt ttttctcaaa cactttcttt
    52381 tcttcagagt ttttaaatat atctactagt ttattcatta ttgaagattt accagcattt
    52441 gtatacccga ctagagctac atttggaaga tttgattttc ttctcatact tctttgagtt
    52501 tctctttgga attttaaatc atccaactcc ctatttaaag atgttatctt ttcttctatt
    52561 cttctacggt caagttctaa ttttttctca ccagaacctc tatttttagt accaactcca
    52621 ccactttgac gtcctaaatt ttcatttgaa cctataagtc ttggtaacat atattttaaa
    52681 cttgctactt ctacttgaag tttagcttct ctagtttttg ccctttgtgc aaatatatca
    52741 agtatcaatg ccgttctatc tataatttca caattaagct tttcttcaag attttttagt
    52801 tgtgatgtag aaagttcatt gtaaaatata actatctcta tattttcttt ctttattaag
    52861 tttaatattt cctcaacttt acctgtccca atgtaaaatg ctctattaac ctgctttgca
    52921 ttttgagtta tactccctac aacatcaata tcacatgctg aacataagtt ttcaagttca
    52981 atcattagtt cattaaagtc ctcagattta ttatttatgt ttaatcctac tgttattcct
    53041 tttttcgcca tatccatatt ctccttatct gctatttttt atttaatcta tagcacgaga
    53101 ttatggctct ataattaaat tcatcaagaa ttttgctttt tatcttatcc ttaaatgaca
    53161 cttcttattt actattataa taaccataat cgtgcattat tttctcaatc ttatataaaa
    53221 aatcactttc ttacacctcc tcccaaatat attatgcagt tttttcaata aaaaaactcc
    53281 ttacaaatct aagtaataca aaacaagcta cttatatcat aagaaggatt aaaattctaa
    53341 atatatatta aaaatacatt agtttaatta acttatatgt aattatcatt ttatatt
//
LOCUS       DAYAOY010000016        51977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYAOY010000016 DAYAOY010000000
VERSION     DAYAOY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.016"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(6..2069)
                     /locus_tag="ACGI6B_001037"
     CDS             complement(6..2069)
                     /locus_tag="ACGI6B_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFG9750502.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2450..5785)
                     /locus_tag="ACGI6B_001038"
     CDS             complement(2450..5785)
                     /locus_tag="ACGI6B_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFG9750503.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5835..6263)
                     /locus_tag="ACGI6B_001039"
     CDS             complement(5835..6263)
                     /locus_tag="ACGI6B_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750504.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6253..6633)
                     /locus_tag="ACGI6B_001040"
     CDS             complement(6253..6633)
                     /locus_tag="ACGI6B_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFG9750505.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6666..7838)
                     /locus_tag="ACGI6B_001041"
     CDS             complement(6666..7838)
                     /locus_tag="ACGI6B_001041"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFG9750506.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7842..9305)
                     /locus_tag="ACGI6B_001042"
     CDS             complement(7842..9305)
                     /locus_tag="ACGI6B_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9750507.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9815..10684)
                     /gene="gatY"
                     /locus_tag="ACGI6B_001043"
     CDS             complement(9815..10684)
                     /gene="gatY"
                     /locus_tag="ACGI6B_001043"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFG9750508.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10704..12620)
                     /locus_tag="ACGI6B_001044"
     CDS             complement(10704..12620)
                     /locus_tag="ACGI6B_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9750509.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12623..13543)
                     /locus_tag="ACGI6B_001045"
     CDS             complement(12623..13543)
                     /locus_tag="ACGI6B_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFG9750510.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13645..14397)
                     /locus_tag="ACGI6B_001046"
     CDS             complement(13645..14397)
                     /locus_tag="ACGI6B_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9750511.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14789..16234)
                     /locus_tag="ACGI6B_001047"
     CDS             complement(14789..16234)
                     /locus_tag="ACGI6B_001047"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9750512.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16256..16609)
                     /locus_tag="ACGI6B_001048"
     CDS             complement(16256..16609)
                     /locus_tag="ACGI6B_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9750513.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16638..17870)
                     /locus_tag="ACGI6B_001049"
     CDS             complement(16638..17870)
                     /locus_tag="ACGI6B_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9750514.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            complement(17893..18210)
                     /locus_tag="ACGI6B_001050"
     CDS             complement(17893..18210)
                     /locus_tag="ACGI6B_001050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9750515.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18452..20365
                     /locus_tag="ACGI6B_001051"
     CDS             18452..20365
                     /locus_tag="ACGI6B_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9750516.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20488..21642)
                     /locus_tag="ACGI6B_001052"
     CDS             complement(20488..21642)
                     /locus_tag="ACGI6B_001052"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFG9750517.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21921..24536)
                     /gene="mngB"
                     /locus_tag="ACGI6B_001053"
     CDS             complement(21921..24536)
                     /gene="mngB"
                     /locus_tag="ACGI6B_001053"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFG9750518.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24680..26605)
                     /gene="mngA"
                     /locus_tag="ACGI6B_001054"
     CDS             complement(24680..26605)
                     /gene="mngA"
                     /locus_tag="ACGI6B_001054"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFG9750519.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26735..27490)
                     /locus_tag="ACGI6B_001055"
     CDS             complement(26735..27490)
                     /locus_tag="ACGI6B_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9750520.1"
                     /translation="MNNKIDGGETMEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVK
                     LSIQSLADKFFTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKT
                     LELIDYEKINKVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVL
                     HDIGKLGEQDMFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLY
                     CYSTIKQVNGYNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27723..28493)
                     /locus_tag="ACGI6B_001056"
     CDS             complement(27723..28493)
                     /locus_tag="ACGI6B_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFG9750521.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28495..29943)
                     /locus_tag="ACGI6B_001057"
     CDS             complement(28495..29943)
                     /locus_tag="ACGI6B_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9750522.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            30313..31035
                     /locus_tag="ACGI6B_001058"
     CDS             30313..31035
                     /locus_tag="ACGI6B_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9750523.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31232..32899
                     /locus_tag="ACGI6B_001059"
     CDS             31232..32899
                     /locus_tag="ACGI6B_001059"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFG9750524.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(32995..34314)
                     /locus_tag="ACGI6B_001060"
     CDS             complement(32995..34314)
                     /locus_tag="ACGI6B_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9750525.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34355..35101)
                     /locus_tag="ACGI6B_001061"
     CDS             complement(34355..35101)
                     /locus_tag="ACGI6B_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFG9750526.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35345..37354)
                     /locus_tag="ACGI6B_001062"
     CDS             complement(35345..37354)
                     /locus_tag="ACGI6B_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750527.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37792..39225)
                     /locus_tag="ACGI6B_001063"
     CDS             complement(37792..39225)
                     /locus_tag="ACGI6B_001063"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9750528.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39312..41228)
                     /locus_tag="ACGI6B_001064"
     CDS             complement(39312..41228)
                     /locus_tag="ACGI6B_001064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9750529.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41302..42162)
                     /gene="licT"
                     /locus_tag="ACGI6B_001065"
     CDS             complement(41302..42162)
                     /gene="licT"
                     /locus_tag="ACGI6B_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9750530.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42513..43700)
                     /locus_tag="ACGI6B_001066"
     CDS             complement(42513..43700)
                     /locus_tag="ACGI6B_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9750531.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43714..45105)
                     /locus_tag="ACGI6B_001067"
     CDS             complement(43714..45105)
                     /locus_tag="ACGI6B_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFG9750532.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(45249..45428)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(45700..47352)
                     /locus_tag="ACGI6B_001068"
     CDS             complement(45700..47352)
                     /locus_tag="ACGI6B_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9750533.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(47372..48775)
                     /locus_tag="ACGI6B_001069"
     CDS             complement(47372..48775)
                     /locus_tag="ACGI6B_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9750534.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49039..49530)
                     /locus_tag="ACGI6B_001070"
     CDS             complement(49039..49530)
                     /locus_tag="ACGI6B_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9750535.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49556..50368)
                     /locus_tag="ACGI6B_001071"
     CDS             complement(49556..50368)
                     /locus_tag="ACGI6B_001071"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9750536.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50381..51334)
                     /locus_tag="ACGI6B_001072"
                     /pseudo
     CDS             complement(50381..51334)
                     /locus_tag="ACGI6B_001072"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51458..>51977)
                     /locus_tag="ACGI6B_001073"
     CDS             complement(51458..>51977)
                     /locus_tag="ACGI6B_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9750537.1"
                     /translation="ENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHI
                     ANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIRQLEYKA
                     NWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINIL
                     KEGLRLITIQNK"
BASE COUNT        16596 a         9549 c         5498 g        20334 t
ORIGIN      
        1 tttcattatt ttatggaaaa tattacttca tttaactcta tatcaaactc attaagtaaa
       61 aatctaatta tatttaattt agcctctgta tttaaatttg tttctaaata tatattttct
      121 gatattttaa taccttttct taatttatat gcatctctag acttaatatt atctataaaa
      181 tctcttttat aagttttgtc tacaatactg ttaaacctta cagaatccaa atcatataaa
      241 gtaagagcaa cttgttcata aaattctgtc caatttttag ccttatatga ctctcctaat
      301 atactaaatg atactatact ttcatttgta aaattaacat catcatcctc taacgtaaat
      361 atattttcac tgttacttgg aggttcatat gtaacacttg gatatttcca aatttctaca
      421 gctctatcta gtaatatctt agttctattt tttatttctt cttcattcca tttatctata
      481 gatgaaatat atttatttag ataaagccta ctatctttaa atccatcttc catttctttc
      541 ttttcttcaa atgttttatt acttagtttt gaattatatc ctgtcaaagt taaatttcca
      601 attgtatgta aatatttatc atgtatctcc ttatagttat ctcctagtga ttccttccat
      661 ttatttgtta atgtctgtgg cattatatgc tctatattta gcatattatt attgattaaa
      721 ttttctaaat caacaatttc tttattacta taattctcta acctctcaag tatatataac
      781 ttattctttg acttagtatt atatatatct ttagtttcaa aagcatttga aaattcactg
      841 tcagtaggaa atctttgaga tgatttctta tttataaaaa tatatcttaa tatattagaa
      901 tagttttctt tataatcact aaatttttta atttcccttc ctaagttcat aaatacttta
      961 ttcagtgcat tcggaggtac atcacatact attcttctta atataaaaca ttctactaaa
     1021 ttaagggatt ctacaagtac ttttttattt attatacctt cattataatc atcaaataat
     1081 tctaatataa acggatatga tacaaatatt tcaagtttgt ttatcctttt caaaactttg
     1141 tttacatcat tatcatcagt acatgaataa gatatctctc tataatagtt agaaaattta
     1201 agtaaatctt ttaataaaat ttctatatct ataccattat ctgatacata ccttttaaaa
     1261 ctaaaatata ctttattttt attcggtata cttctctcct ttatagtcaa atagtctctt
     1321 ataaaactac ttacgttgct acttgtattt ttttcaattt tataccaata attattataa
     1381 aattcctctt gtaacttaga gcttctaccc attaacacaa agtttcttac tctatcagct
     1441 tcacttaaat ctaagccagt tgaatttaga ctttcaaaga ttaattgagg gtcatcttca
     1501 ccatttttta actctatttc tactatagtt aatttctgta tagcatcaaa caaatcatct
     1561 attgatatat tatcttcttt tattctatta taaaaatata aataatttga aactatatta
     1621 gaatttctat ctgtattcat ttcttcttca aatagttcat agtacgcttt tctatcttct
     1681 gctattggtt ttaactttat ttttccatct ttatctcttg agtgcttgtt tataagataa
     1741 cactccttaa tctgttcttc tgttgtaata attccttttg attccccaaa ctcttttatt
     1801 atttgacaca tggcaatcaa taatattgac acagtagtca ctctttgttg tccatctatt
     1861 attaaatatt cttttcccat actatatata gatacaactg accctaagaa atgagttcta
     1921 aatccagaat ctattatatc ctctaaatca tcaaacaatc ttttacattg ttctttcttc
     1981 cagttataat ttctttgata tacaggtact acaaaacttt tgtttgaccc ctctaaataa
     2041 ctaagaaatt caatttctcc tgctttcata ttaactcccc ctataagtta aataaattcc
     2101 attcataact ccatgtatct atatattata tcaaaatttt gactcataaa tgtatatgaa
     2161 agccttatta tatacctttt tatacctgtt tttattttat taaagtaaat acatgattat
     2221 tctataaaat atacaaatac ttaattttaa aaattattat ttaatcttct tcatgagtaa
     2281 ttcatagtta tttatcaatt aaaaagagat attgcataat tattcaatac ctctctttgt
     2341 atatttacat tatccatatc tagttatcta tttcaaccca atattagact acctactaat
     2401 aaatttattc tctacatcat taacaataat tttgcactaa ataacatcat tagcctatct
     2461 ctgatgcatt ttctttaatt ttatttatag ttttaatcaa tttatttctc tcttcaatat
     2521 gattttcaaa taaatcaatt atacttccaa cagttctaaa tgggtcttcc atcaacactt
     2581 tgttatcctc tataaatcca ttcttgacta cataatcgac tataagttta acaaaatgta
     2641 tttgcttagt attaaagctc ttattattta aaaactctga aaaagcttca tttgcagctt
     2701 ctctatccaa tcccactagt cttctcacta attgacttac tggagtatct ccaaactcct
     2761 tcttataatc atcacaatta cctaactgtt ttaagagtat ttcctccaac gtttcaacat
     2821 cttgctttgt tatcttttta ttatttctta atttaaatat tgctaattca tctttatggc
     2881 tatttaaata atactctacc ttctttttat aattcttaag attgtttgca ttatatacag
     2941 aatcatttct atcttctgct acaatcatat cttcaaaact tgtataatat atcttttgac
     3001 tagacttttc aagatactta ataagctctc ttaatgatat acgtacttct tctacctcaa
     3061 atatatctga atcttcccaa aaattctctg tcattgcttt ttcaatagta tatttttgtt
     3121 gctgaatctg tggtatagtc ccaagttttg aaagtttttc tgctgtagcc attacatgcc
     3181 ttataccttt acttgcatta ttattttgta agtatgcaag ttgtatatta tacataacta
     3241 catcaaatct tttagccaat tcatcatcac tatatgaagt tatgagtggt gatatatatt
     3301 ctcttatctc attttgtgct actgctccaa tattgctcca aacattttca tttttatact
     3361 tatgtacata acttaaattc atcctaacta tataactatc ctcatttaaa gcttttacag
     3421 aagttataag ttcttctaac aattcttttc taagttctgc atactcacct tctatatatt
     3481 taatatcttg aagttcttta accatactta cctttatatt aaataatttt tcagtaagtg
     3541 tttctgcttt atttccctca aatcctttag gattaaattt aaaaaattca aaattattac
     3601 aaaaatcaaa tattaagaat ctctccttat ccatatcaac acctaataaa tctggacata
     3661 atctagttcc tctacctatc atttgccaaa acttagtctt acttctcact ttcttgaaaa
     3721 aaacaagatt tagtatttca ggtatgtcaa ttcctgtatc aagcatgtct actgaaactg
     3781 ctatttgagg taatttattc ttatcagaaa aatcatctat tatagaatct acataattta
     3841 tactatagtc aactgctttt acaaagttcc caccatactt tggatacagc ttattaaatc
     3901 tttcaactat tgccctagca tgctttgtat tctttgcaaa aattatagtc ttaccaatct
     3961 tttcttctcc ttctattttc aacccctctg tcattagctt atttaaaact aaatctattg
     4021 tgtcagaatt aaatagccac tcatttactg cattattccc tatttcgtct ccaatactct
     4081 catcatcatt aaatgtattt tcatattctt ccttctcttc atcagaaagc tcatcatact
     4141 taattccatc ttccataatc ttagtcttaa actcaatact agtataatct actaaatatc
     4201 catcctcgac tgccttatta tactcataag catatgtagg tactccattt tccatgtcaa
     4261 aaactgaata agtattttta tctatctcat cctttggtgt agcagtaaga cccattaagt
     4321 aagaatcaaa ataatcaaag attgacttat actttttata aatacttcta tgcgattcat
     4381 ctattataat taaatcaaaa tgaccaggtg taaacaatct ctttccatcc ttagacttcg
     4441 tatcatcaat tgcattcatc attgttggat atgtagaaaa tatcatccta gcatcttctg
     4501 ggctgtcttt gctatccaat aaattacata acgccaagtt aggtaatagc ttagtaaaac
     4561 tttttttagc ttgctttacc aatgcttttc tatctgctag aaataatata tttttaatcc
     4621 aattatgtct tgaaagtaca tcaactatag atatagcaat tcttgttttt cctgtaccag
     4681 tagcacatac caaaagagtt ttgcgttgct tattttcaag ggcctcacat acagcccgaa
     4741 tagcttcttt ttgataatat ctattggata tttcatcatt tattgttaca gaacttaaag
     4801 acttcttact ctttcttcta tccatcataa gttgtaattc tgatttttta taaaatccat
     4861 aaacttttct atctgaatag tcatcccaaa tatacatatc aaaaccatta gtcaaaaata
     4921 ttactggtct ttgactatat ctcttttcca aacaatctgc atataattta gcttgttgtt
     4981 gaccaatttt tgggtctttg ctcgttcttt tcgcctcaac tactgctaat ggcttaccat
     5041 tttcaccata aagaacataa tctacatatc ctacaccact ttcattaggc ataccttgta
     5101 cttctatttc ctcccctata tcttttccaa attcccaacc tgcaagcttt aattctatat
     5161 ttatatatct ttttcttgtc tcaaattcgc ttacttcgtc tatcttgaaa tcatagttta
     5221 cagtattaac ttttctcttt tcagttagtg actttcttaa ttcctcattt tcttttatta
     5281 cttcttcaag ctttttatct tttgaactta atctctcgta aagattttta agttcgtctg
     5341 gtctcgtcct tttctcttct ccatgaagta ataaatcttc attaaagtct tctgctgtat
     5401 attcatctga ataacaatag tctatccaac taacaaattg atgcaaattg ttaagtgaaa
     5461 gtattgcttc tcctctctct atgttcgcac tagtatgtac agataaatta cctaacttaa
     5521 ctatatattt taataaaggt aacatatcat agtctataag ttctataaaa ctattattat
     5581 gaatcaacgt actgagatta tcttgataag gcaatactaa atcactatca ttagcatata
     5641 gccattttac tgcaagctct aaagctcttc ttgttatagt tgcacaagta gatggactaa
     5701 ctagtatact tttttctgct tctaagcaag cagtattaaa actattgaaa tttttcttct
     5761 tttgtaaaaa atcaaaatta cccaaggctt ttatccccct tttgtccctt agttaaaata
     5821 aatctctcta aaaactattt cttacagctg tttcttatac taacaaatac ttcttcattt
     5881 tctgtaaaca attcgtacat atcttcaaac tgtggtatat catatccatg agaatcatca
     5941 tttctcaatt ttctaagtgt ttctataact atagaagaat ttttaggtaa taaattgtga
     6001 tactcacaag ctaaaaggta gtcataaaag tttttagatt ttatgtttaa atcaattgat
     6061 ttataataca acattgcttt tgttaattct agaaatgata ccatatagct tttaaagcct
     6121 tcttttatgt atctagccca taactcaggc atttcttcct tatcatataa gattttgaag
     6181 ttttgcaggt catacaagca agaaccaaaa tccctattta cttcttctat tgagttccaa
     6241 gataatttgt ttctaattac catacatgtc cctccaatct aagatatcta ttacatcact
     6301 gttttcaaaa tagaatttat ttatataatc cttaaaatct ttagattcat aaataacttc
     6361 accttctaaa atagcttttt taagtgctat tgtataatta tctgtaacaa tttttaggtc
     6421 tacatcaatg tctaatatat ttcttagagc ttctgtctta gttgcaagtt ctgtaattgg
     6481 tacttttttg atgggaatcc aagctatgtc tatatcactt tctgatgtat gtttatctgt
     6541 tccataacta ccaaatatta tgattgctag agtattttct tttggaaata catttattat
     6601 tgagttttta atctcatttg gtatatttct catttaatca cctcttgttt attaggtaat
     6661 attgtttagt taaataattc tcctttgaaa gctttttgca ttaatgaatt aaagttatct
     6721 tctaactctt ttaagttggt ttctattttt aattttaatt tatccacttg cttaacaaac
     6781 tcagaaaact gattttgaaa ttcaattgga ggaaccatta ttaaatattc acctaactta
     6841 ctaactgata actcaatttg attcgttgaa ccattaacac attttctata tattgtgtct
     6901 tgtatatttg taaattcaaa taacgctgca aaataatctg aatttaagtt atttgaaata
     6961 cttcttaaaa ctgtaacatg actatctgct atatattcag aattatcaat tttgttaaat
     7021 tttacacatc ttcctaatgt cccagttcca gtagaattta ttaatatatc attttgtttc
     7081 aaaaatatat cttctatctt ctcttttaag ttttcatcat aatatttaca attttctacc
     7141 ttcaaatttc tccagtatat acatttttga tttataactt ttatatttga ttgctctaca
     7201 tatttaggag atacacctct ttttaaatat atagaaatct ctttaatctt cttcatatca
     7261 aatttttttg tattattaat tggctcccca aacatctcaa taaatcttga tttaactaat
     7321 tcatccaatg cttctatttg ctcttttctt ttatttatta actcttgagc tttatttaaa
     7381 acttctacca ttttattttg tatacaaatt gtgaaatatg gtatttttat atcatttaac
     7441 ttgcttttat ttaaagttgc acccataact gctctatcag tattattaaa atttaaagtt
     7501 ctcaaagcat aatataaata ttcagatgtt ataagttcat tattttttat ttgaaagtta
     7561 gcaattgctt catttgtaaa catgtcttct tttaatatgg caactctacc tatagacaac
     7621 ttaaaactca ttacaacggt atttttaggt actaatttca tattagcttt ttcaacagct
     7681 aaatctgtta tctcttcttt tgattttgat atatattttt ctttcaaatc agaaattgat
     7741 aaccatctat taccacttcc ccaataatca gaagtatttc tagatggtgt tttgccaata
     7801 tttatatagc acaattcatt taattttata tactccatac cctatacctc taacattctt
     7861 tctaattctt ctaatccaac agctatttcc ttctctaact ccctaactct acctataatc
     7921 acactaggag catcataaac tacttcctca tacactacct ctttatactt attaatagat
     7981 aaatcatacc cattctctct tatctcttcc acatcaacaa aaaagctttg ttctgttctt
     8041 tttctatcta cttccccatc aagattatca aatctcttaa taatatcctc tatatcacta
     8101 tcctttatct catttctctt atcatccaaa gaataaccat cagctttcat atcatagaac
     8161 cataccttat cagttccacc tgtccctgtt ttagtaaata tcattatagc tgtagatact
     8221 cctgcataag gcttaaacac tccacttggc attgatatta tagcttccaa tttatgatta
     8281 tcaactattt cttttcttat cgatttatgt ccacctgttg aaccaaacaa tactccatct
     8341 ggcactatac atgcgcatct tccaccaatt tttaaaactc ttaaaaaaag tgccaagaat
     8401 aataactctg ttttctttgt cttagtcact tttaataaat ctgctgacac tgcttcataa
     8461 tctaaacttc ccttaaaagg aggatttgct aacaccaatg taaacttatc tttatctgta
     8521 ttaacctccg ataaagaatc cttatactct atattaggat tgtcaacgcc atgcaacatc
     8581 atattcatag cacctattct aagcatagtt ctatccatat caaatccata gaacatacca
     8641 ttattaaaat gctccttcag cccttgcact aagaaaagac ttgaatgatt atcccttaag
     8701 tattgttgtg atgatactaa aaaacctgct gaacctgctg ccgggtctac tataatatct
     8761 tctggtgttg gtttaataag tcttactatc atatctatta tgtgtcttgg agtcctaaat
     8821 tgaccattag tacctgctgt agccactttg gataataaat actcatacaa atctcccttt
     8881 gtatctctat ccttacttaa ttctaaatta ctaataccat caactatttt agataacata
     8941 agaggcgtag gaattttaaa tattgcatct cccatgtact ttgcataagc tgagtcttta
     9001 tttccatgta atttttttat gaaaggaaac acttcatttt gtactatttc atacattttt
     9061 tctgaatttc cttcattaga aaacttactc catcttaaat gttgatgttc atcatcaaat
     9121 atcctttcaa aagaaactcc caaaagagtt gcttctgctt cattttttgt ttctacttca
     9181 tccaatcctt taataaataa aagatatgta aattgttcta taacttctaa tggattagtt
     9241 aatccaccag tccaaaatgt ctcccaaatc ttgtctactt tgctccttaa ttctcctgta
     9301 atcatatatg actttccctc cagtgttcat aaaataattt aaatatatat tttagtcgta
     9361 cgccttaata attagtgtct tatattaaat tcaaaatttt actacacagt tattaaatta
     9421 tttttcctat ttacatgttt tagttaaaaa actaatgcaa tactcttatt ttatgattat
     9481 tatacatccc atacaaaatt attttatcat atatccagca tagaagtcat ctacctggat
     9541 aactcttacc atattataac tttttaatat taatttaaac ttagaaagaa attttttatt
     9601 taattatatc atcttcctta tttcaaattt ctcaattaca atctcaatca aaaaataaaa
     9661 taagatgctg tctcaaaata gatataatct tcttttttat tttcggaatg atgaaaatat
     9721 atttaatttc atacaaaaaa gagtgcctcc tgaactaaaa agttcatttt gagacacccc
     9781 ttaatttaat cttacttatt ctctcccttt attattaaca cttcactatc tctattgctt
     9841 ttcctacaca tccacaaact tccatcctac tcttaacaaa atctttaact gactctaaac
     9901 cttttttacc atatttctta gggtcaatta cctctgggtc agaattaagc acatccttaa
     9961 ccccattgct ataagcaatc cttaattcag ttgcataatt aaccttgcaa atccctcttt
    10021 ttatagattc cttaataact tcatctggta ttccagaccc accatgtaat actaatggaa
    10081 ctgatacaac atctctaatt tcagataatc tatctaaatc aagctttggc tctcccttat
    10141 ataatccatg tgccgtacct attgctactg ccaaagatga tacccctgtt ctctctacaa
    10201 actcttttgc ttctaatggg tctgtatatg ctcccccatc accaccatct aaatcgtctt
    10261 ccttacctcc aactttacct aattcagctt caacaggtat atctgaaggc atacatgcat
    10321 ctacaacaga tttagatatt gaaatattat cttcgtaact ttcatgagaa ccatcaatca
    10381 taatagaagt atatccaact ctaagtgcct gcatagctag tccaaagctt gaaccatggt
    10441 ctaagtgcat tgctactgga acacttgcct tctctgatgc tacctttaca ttcgctaaat
    10501 aataatctag ccctgcatac tttattgttg aaggtgttgt ttgcaatatt actggtgatt
    10561 taagttcttc agcagcctct attactgcca taaccatctc catattttca acattaaatg
    10621 cacctactgc ataatgtcct tcttgtgcat ctaataatat ttgtttagtt gtcactaatg
    10681 ccataatttc tcctcctata tgtttatact gatttgattt cttcgttatt taattttgct
    10741 actatttctc tacttgtttt gctatttaat aactcattta aaaattcttc attcattaat
    10801 tttgaagata gttgagataa tgtttttaag tgtaaattat caccattatc tgtagctgct
    10861 atcataaata taatatgtgc taagcttcca tcttctgatt caaagtcaaa cccttctttt
    10921 gatacaccta ctgctacacg tggtaccttt actgcatctg tttttgcatg tggtatcgct
    10981 atccccatac caaaacctgt agatgatatt tcttctcttt taaggatttc ttctttaaac
    11041 ttctttttat ctaaaagaac actagattta tcaagtattc caatcatttc atcaattaca
    11101 tctgttttat tttttccctt aacatctaaa attactgttt cttcgctata taaagagata
    11161 ttattagata cgttttccga ttctgcactt tcatttttat gttcatcttt tgttacattt
    11221 gtattttcta tagtattttt ctcatttact actttaggtg taaccatcat ataaagtaca
    11281 gcacctacta atgaacctac aaatatacac cctacccata aaagtggctt attgactaat
    11341 cctagaacca aaaaccctcc atgtggagct ggtacttcta ttttcattat ataagttaaa
    11401 attgatgata ttgatgatgc aatcataagt actggcaaca cttttaatgg attttttgct
    11461 gcaaaaggta ttgccccctc tgttatatga gtactaccta gtatataatt gattaatcct
    11521 gctgctctat cttcttctgt aaatctattt ttaaataaag tagatgctat tgctattacc
    11581 aatggtggtg ttatacaagc tgcagataca cctgccataa ataaataatt tccctgtcct
    11641 agaagcatag ttccagttac atatgctgct ttatttactg gacctcccat atcaaatgct
    11701 gacatacatc ctattactat tcccaaaatt atagggcttg aattttgtaa ccccgataga
    11761 aagttcatca tagcattgtt aagtgctgaa catggcccac caagtaacaa cattgctaca
    11821 cccacaagac ctacgctgac aataggaatt ataaaaatcg atttaagacc ttctaattgt
    11881 cgtggtaatt ttcttaacaa atgtacaaat agtttaacta agtaacctgc taaaaatcct
    11941 gatatgattc cacctaaaaa ccccgaacct gtatcaaatg atatcattcc tccaacaagt
    12001 cctgctaaca taccagctct tcccgatata gattgagcta taaatgctgc caatacagga
    12061 accatcaagt tcatagaata tccaccaaca gattttaatg tagctgctat ttgattatat
    12121 tgttctgaat ttgggtcaaa tgaataaatc ccccataaaa atgatatagc agtaagtact
    12181 ccacctgcta ctactaaagg taacatgtga gatacaccat tcattaagtc tttatacaac
    12241 ttttgaccaa acgataaatt tccttcttct gcaattttac tgcttgataa gtttcctttt
    12301 ctaatagcta cttttccatc aatacatctt tgtataagtt cattagcttt atgtatacct
    12361 tctttgacag gtacttctat tactggcttt ccattgaatc tgtccatatc aacattttta
    12421 tcacaagcaa ctattattgc atctgccatt tcgatatctt ttgctgttaa cttattttct
    12481 acacctatag ctccattcgt ttcaactttt atgtcacacc ctaattccct ggctgaattt
    12541 tttaatgctt cttctgccat aaaagtatga gctattccag ttggacaacc tgttacccct
    12601 agaatcattt tctttcccat ttttatacca cctttctgac tcttatattt tttatataat
    12661 cttccacatt ctccaaatta cctataccat tactttcagc cacacttgct cctgttgctg
    12721 ctgacctttt taatgcttct tcaatatgtt ctggattttc caaccatata cttaaaaaag
    12781 cagctaatgc agaatcacca gcacatgcag agctgactac tgtaactggt tgagcactag
    12841 cataataaat tgattttcca ttataaaaat aagaaccttt ttctcctaaa gttagcaata
    12901 tattttgagc accttttaaa tgtagtaatt taagtacatc cttaatatct tcttcatctc
    12961 ttacaataat tccaaatata tctttgattt cttcatcatt tggttttatc aaatatggat
    13021 tatactgtag taactccttt aattttggtg aacttatatc gagtattgtc tttatacttt
    13081 tatttttaca aattttaaat atatcctcat agtaattatc atcaatacca ggaggtagac
    13141 ttccacttat tgataagtaa cttatatctg ctaatgactg tatcttatct agcatatcta
    13201 gtttttgttt ttcatttaca tatgacccag agtttacaaa tttaaattca tcacttccat
    13261 catttaaaaa tatatttata cgagttgtat cttctaccca agtaggcaat acgtcaaatc
    13321 ctctcttttc gctttcttca actatatatt ttcctgaaaa tcctccaaaa aatcctaata
    13381 tcttagattc tataccataa tgacctagta caaaagttac atttaatccc tttccattag
    13441 gagaatatac agcatttgat gtacgattta ctaattttct ctttatccca ttagtagata
    13501 tattcatatc tattgcagga tttgtagtta atgtatatat cattgtcttc ccccttgctt
    13561 gtgctgtgta tgttttcatt ataataaaaa agtctatgaa aatcttttca caaatagaaa
    13621 atcgattttc atagactttt caatttaatt ttcagaatta ttatatctga ccatataatc
    13681 tataattatc ctagatataa tatacaaggg caatctggaa gatacttcat attcagaaat
    13741 tgatatatgt tcatgtttgt atcctacaat atataaatcc gaatacttta taagttttgc
    13801 actttcacta caacagcaag ttataacctt agctccaact aaattggcat tctgtgctga
    13861 ttctacaagc tctatggtat ttccattcaa tgaaaatata attataagtt cgtctttttt
    13921 tattgattta gattttattt tcattatgtt ggggtcattt ataaaaacaa cattaaaatc
    13981 aagtaattgc aatttaagac taaactcttt tgctacatat tcagtaagcc ctcttgctaa
    14041 aatatatatc ttactagatt tatttatagt ttttattata ttgattagtg tagttaaaga
    14101 tatttgttct ataactctct gtgcttctat aaaggattta tttattatat cccccatatt
    14161 ttgtatatca tcattttcat cttttactgt taatctatac ctaagttcat tgaatccatt
    14221 taaaccacat tttctaattg ctcttgaaac ggttgctgga gatgaatatg tttcatgagc
    14281 aatctctact atagataatt ctggtaatat tgattcattt tcatttataa atttaattat
    14341 ttcaagttcc gtcttcgtta gattttcaga attacttttg tttagctcaa ttatcatatg
    14401 ttatctcccc ttatataagt tttatttgtc taaatttatc gagtaatata atatattacc
    14461 tcttaactac ttatttttat atacaattaa gatttagtca atgtctatat atgtaaattt
    14521 ataatataat aattttttgc ttaaagttta ttatgttatc ttatagcatt ttaaaataaa
    14581 aaagaattgt acctctacca aaatttggat gaagtacaat agatattcct catactgata
    14641 tgtttatgat taaaagtata aatataaatt tcgctatgaa tcatataatt aataaatcat
    14701 tttatttaca attataaccc cccttataaa actatattaa taatatatac agtatttata
    14761 aggggggttg tttatattta aaaactattt acttttctct taattcttct ccattagttc
    14821 ttattacatt ttgataccaa taaaaactat cttttttata acgttcttta cttccatttc
    14881 cattgttatc tatatccaca tatataaatc catatctttt ttccatttct tgagatgaac
    14941 aacttactat atctattggc gaccacatac aataagctaa tatttcggtt ccatcagcta
    15001 tagcttcacc tatttgttct atatgttttc ctaaataatc tattctgtaa ctatcatgaa
    15061 cttttccatc ttcaaattta tcatgcattc caaatccatt ttcagctatc attattggct
    15121 tttggtatct atcataatat tgagataaag tattgtataa tccttttggg tcaattgccc
    15181 acccccaagg atttgctttt aaatatggat tctttatatt gtcagttgga ttcatggtgt
    15241 ttttacttgc tgatactgtt gtagaataat agtatgataa agctagaaaa tccatagtat
    15301 tttcttttaa gattttatca tcaccttcct ccataactat atttatgtta ttttctttga
    15361 aaaatctctt aatatagact ggatactctc ccctaaaact tacatcagtg aaaaaatatt
    15421 gtagcctatt tctttttaaa gcaaatacta cattttcagg gtcacaatct tctggataag
    15481 atgtacaatc tgccatcatc atacctatca tcatatctga atttatttct tttccaagct
    15541 tcttagtcaa agctgtagct atgaattgat gatgaatagc ttgatatcta gtctcttcta
    15601 cattatcaac ttggtctaca caaaatgcaa ctgaattaaa tgattcataa tgtattaaat
    15661 ttatttgatt tataactatc caatatttaa ctttattttt atatctattt agtattaatt
    15721 ctgcatatct aacaaagaaa tctaccactt ttctattaga ccatccttta tattttaata
    15781 ctaaattcaa aggagtctca tagtgaagta gtgttacaat aggttccata ccattatcta
    15841 ttacttcatt tataagatta tcataaaact ttaatccctc ttcattaggc tctaaatcat
    15901 ctccatttgg aaatattcta gcccaattta ttgaaaatct aaaacacttt aaccctagct
    15961 ctttcaaaag ctttaaatct tctttgtatg tatgataaaa gtctattcca ctacgcttag
    16021 gataatatcc attattatct ttcatagcaa actttattct ttctatagtc atatcagact
    16081 cataaggttt agatatatct ctatctttat catacatatg tacatcagct aaacaccaac
    16141 ctttgccacc ttcattccat gcaccttcta attgattagc tgctacagct cctccccata
    16201 aaaaaccttt aggaaactta cccaatttaa tcactcctta ttttatttat ttaaatcatt
    16261 tactattatt taccttatat tctatagact ttactattcc tactaaagac atagttatat
    16321 ttaactcgct gaatatggtc ataagagtat cttgagcatg tgcaaataac agcgaatgtg
    16381 ctcttttctc tccaccagct tccccttgta taatcttggt ttgagctcta tgtgcttctg
    16441 tcataaaccc ttttgcttta gctagtttta aatcagcatt tctataatca aacttttcca
    16501 aatcattaag agcatgttta atttctgttc tagcctcacc tgcacgaagt attatttgca
    16561 ttgctacttc tactaattct tcatctatta tatgttcttc aattgtcata tttataattt
    16621 cctttcaatt tctaaattta aacctcttga ctctctaatt ttatctcttc tttctcataa
    16681 gctttaaaaa atggatacca tatcataaat agcaatacta tatttattgc taaaacaata
    16741 agacctctaa catcttgatt taccataaaa gtagatatac caaaaggaac ataccataaa
    16801 ttaaacacct tacttggaat tgtaacaaat cccatactca ttgataaata agtagttatt
    16861 gatattacta ctgttcctat ccacataggt aacattaaaa atgggttcca tgccactgga
    16921 gctccaaata ttactggttc atttatattc attatagaag ggacaataca tgctcttcct
    16981 attgctttta ttcttccaac tctggataaa cacatcatta taactaatgg taatgtagca
    17041 cccaaccctc ctaaagttat ccagcctgtt tgaataactt cgtaaacata tatattagtt
    17101 gctactcctc ctgatgctac tgctgctgca ttagctgcaa ttgcaccaag ttgaattgaa
    17161 taagttatag gagatagtac ccacccactt attcctaaag aatatataaa tactactaag
    17221 aagtttaaga gtacaaatcc aggtaatgtc tgtgctatat ctgatactgg tttaaatact
    17281 ctaactatta tttcaaacat gtcaaagttg agtccataaa ttaatcccca gcctacaaac
    17341 attatcactg ttataggtaa catattatca aaccacttgc ttacaaaatc cggaagtgct
    17401 gaatcttctc cgaaaaatga aaaagaacca aataaagaca ttattaatcc agagaataat
    17461 cctgcaacta gcgatacaag cattccttct cctccaaacc tgttgaagtt aaatatagct
    17521 ccttcattcg taatctctgg acgaatcatc attaacatta atccagcacc tgcaagacca
    17581 gctactacac tcatactttc aaatttctta ttcttcattg tttccatagg caataaaaat
    17641 gctataaata atcctaaaag tccaaatgta tattgattta ttggacttaa atcaggtaaa
    17701 aagcttatgt aattcttcaa gacagaaact atagttacta atgagcctac aaataccatt
    17761 ggcaaaactt tcattatagt tgattgaact gattgtaccc atatattctt agtcatatta
    17821 tttacaacag gagtaaattt ttcacttaaa aaatctataa atttattcat aaacactctc
    17881 cccataaata ttttatttgt ttgatattag ttctagtgct tcttccaaag cctttgcacc
    17941 atctagtctt ccatatgcag ttttagatat acatgctact ggaacgttat attcattagc
    18001 cttttgttga atttcttcct tcatataatc taaatgtggt cctagtaata aacaatctat
    18061 ttctgataaa tacttatcta tctctgactc acttctcgct tttatatctg cttctatacc
    18121 ttttttcttt gcactctttc tcatactagt tgccataaaa cctgtacttg ctcctgaacc
    18181 acacgccaaa agcactctta tatttttcat aataatacct ccaatatttt tatccattaa
    18241 tttatttctt aatgaacttt ttattttatt atgtttatat tttacttccc aaataagttc
    18301 atttcacata aatatatttc ctaataatag gaaatcattt actcttaatt agacctatta
    18361 atatagtata atgtatctta taatacgtac attcaattat ataattatca tatcatacag
    18421 attttatttt aaaactatgc gagggaatct aatgaaaaca tctaaaaaag agctattttt
    18481 aaatttcata aaaacgcaac atgattggat agattcatcc acattagcta attaccttaa
    18541 tgtatcaaca cgttcgatta gaaaatatgt aaacgagatt aattctaatg gtgcatttat
    18601 attatcttct aaaaagggct ataaagtaaa tctcaatact aattgtcaaa ctaagattga
    18661 tagttcagag aatatatcac ctgataacag attaaattta attttaaaag aacttatagt
    18721 taatagtaat ggaattaata tatttgattt aagtgaagaa ttatttgtta gcccagctac
    18781 tattgaaggg gatatagtca aagctaataa atttatagga agttataatt taaagataaa
    18841 gcaatcgaaa tttttattga aactagttgg aaatgagtct gcaaagcgca agttaatgag
    18901 ctctataata tttaaagaaa cgggaagtga ttttttaagc ttatttgatg tacaaaaaat
    18961 atatcaagag tataatttga ctaaactaaa agaaaatata atatatatat taaaaaaata
    19021 caatttattt ataaatgagt atgctataaa caacatactt cttcatttaa tgataactat
    19081 agacagaata aaaaagaata attacataga ctctgttgaa gtagtaaact acatagacaa
    19141 taacgtagat ataaatattg caaaagacat atcaaatttt ttagaatctg aatacaatat
    19201 aactttaact agtgcggaat tatactattt agtatttcag ttaacaaaca aaactacagt
    19261 cttaaactac aatcaaatgg atactaaatc attgtcaaat tatatagatg aacactttgt
    19321 taaattaact aaaaaaataa ttaaaaatgt atatgactta tatcttatag atttatctga
    19381 tgaggaattt gttgtaaaat ttactcttca tgtaaaaaat ttaatcagta gagccaaaaa
    19441 taatcaagtt ttaagaaatc aaatacctca aaagttaaaa gattcctacc ctttgatata
    19501 tgatatatct gtttatattt gcaatcagat tcaaacttta gaaaatgtag atatagacga
    19561 agatgaaata tcttatatat ctcttcatgt aggttcattc tttgatagac aaaaattatt
    19621 agaagataaa gttctatgtg cattaattac tccaaattac tatgatttac aattcaaaat
    19681 agtaagagac cttgagaaga gatttaatga atccatagaa attattcaga ttttttcaga
    19741 tactcataat cttgattttg acaataaggt agacatggtt attactactt taccaattaa
    19801 cgatagatgc cctatacctt ttgtttatgt aaatccttac ctaaatagaa aggattatga
    19861 taatattcaa agtaaattta ttcagattaa agatagaaaa aatatactta ctgtacaaaa
    19921 tcatttagaa atgtacttta gtgaatcttt gtttatgaaa aatatatatt tagatagtgc
    19981 taaagattat ataagattta tgggaaatat tctgtacgaa aataaatatg tcaaacccaa
    20041 ttatatagat gatgttctta taagagaaaa aatgtcttca actgcattta ataacaatgt
    20101 agcaattcct catagcatga aaatggatgc tttaaaaacc ggagtatgtt taattgttaa
    20161 cgataaacca gttaaatggg gtgaggaaaa agttcaaatt atagctatga tacctataaa
    20221 tgaaaaagaa aaagaaaaat tcaattacat atttgaaagc tttattgaaa tattatctga
    20281 atggaataat gttaaagaac ttactaaggc agataactat agtagtttta tgaatagaat
    20341 agcttatctt attcaaaata tctaaatcca ttaatttatt tccaatatat gtttcttaag
    20401 ttattaacat aaaatagata agctgtcaca ctaaaaagtc taaattaata tgtgacagct
    20461 ttttacctaa tataccttgt tttaatacta catatttttt gactgttgta tactagttat
    20521 aagattgaat atttgtgttg ttgtagctgt catattctca ctagcagttg ctttatccat
    20581 tgcttcttct attgtcatag ctctatttac tatagaaaaa taagcatcta taccctcttc
    20641 attacattta actgcatctt ctgttgtggc tccagccaaa cctattactt ttaccccata
    20701 tttttttgcc agttttgcaa ctcctattgg agccttgccc atagcagttt gattgtccaa
    20761 tctaccttca ccagttatga caaaatctgc atcttttaac tcttcctcta atttaatttc
    20821 ttctaatatt atttttattc cagattctaa ttttgaatct aaaaatgcta aaaaagcaaa
    20881 acctaatcct cctgctgctc ctgctccttc tacattagct acatctttac ttaagtaatt
    20941 tttaactact ttggaaaaat ttctaagccc attatctaac ttctcaatta tttctgatgt
    21001 tgcacctttt tgagacccgt atatgtatgc agctccattt ttcccaaaca gcggattatt
    21061 tacatcacag gctattttaa acttacattc atctaattcc tttaatctat tttctatttt
    21121 tatatgtctt atttcattta aaacttttcc acctaatcct actaacttgt tatttttatc
    21181 ataaaactca aaccctaagg cttgaagcat acctatccca gcatcattag tagcacttcc
    21241 acctatccca actataaaat ttctatagcc tttatttatt gcttccttta ttatttcccc
    21301 aacaccataa gttgtagtgt ataaaggatt tctaagttca gtcggaacca atgttaatcc
    21361 tgatgcttga gctatttcta ttattgctgt acttgtattt tttaataatc catatgttgc
    21421 atccactttt ttagctattg gtccagtaac ttctatagtc tcttttttac cattcatccc
    21481 ttgaactaaa gcatctactg tcccttcacc accatcagca agtggcaata tctttacttg
    21541 cgcatcttct ttaaccttca atatcccctt ttttattgca tttcctgcct ctattgaaga
    21601 taagcttcct ttcaaagaat ctatagaaat taatattttc atacttatct actccttttt
    21661 attactcctt attttaaaca tataatttta taagcttaat ttatctttta ttttatactc
    21721 tacatctctc tttgtataca acttatacca ttaatattct tttatcttat aaaaagggag
    21781 tgtctcaaaa taaatttctt agttcatgag acactctttt gtataaaatc aaatatatgt
    21841 ttattatttc aacaatgata aggaggatta tctctatttt aagataattc ctttttagca
    21901 ctatctaatc atttttagca ctatttaacc agtatagttt taacttggtt cattaagcac
    21961 tctacattta cagagttatt atctattgat atagattcta attctttttc atttaggtta
    22021 atccatttag ctgttttaat atttttgtta aatactatgt tagctgattt tgtttcttct
    22081 ttagaattat aaaatctaac tactaatgcc tcttcttttt cagccttttt tacaacactt
    22141 aacacaaggt ccttattagt ttccttaagt aaactatatg aataatctac ttctatttct
    22201 gattgattca atttcatagc attatatggc attttattat aagtttgtat ttttgttaaa
    22261 tactctttag ccatatttgc aacatttgct tctagtgtag atttttcatg agtaactatt
    22321 gctaagtcta tttttatatc tcctatcatt tgagaatctg gagtaggcaa ctttattcct
    22381 gatggacgac ctgggcgtct tgccatttct tctttaccta ggaatcctac acttctaaat
    22441 aatgttatag ctattgtgtc aaatttttcc cctactattt caaactctct tgtactgttt
    22501 gtaagaacag ctactccatg ctctttgtca gacaatccaa cgaatgttag cattggatat
    22561 atagaatctg gtctttcact ccagtcttcc tgttgccaaa cttccatagc atcatcatac
    22621 acacttctct ttatatgtcc aaactgatta tcagacacag aaaactttga agctatattt
    22681 gttggtatat aagttctaac cctacaatct cttgcttggt tatttatgtt gaatactaca
    22741 tctattatag ctttatcatt tggaacattt ataactatat caaagtcaat aaatgaatct
    22801 gctctattat tttttctagc ttgtatatct tttggtactt ttaatctata atttatcttt
    22861 atctcattat taaatctatt tttctttata gtggaatttg ctactatatt atcgttaaat
    22921 attaatttct catttggaag tggtgaaaaa tcatactcat ctccttcatc tcctccattt
    22981 tccatcaata atacattttc aaaagtttta tttaattttt tatcaaatat ctttaatgtc
    23041 ccgttttcat taacttgaat agtataaaag tcattatcta ccatgtcaca tacttcataa
    23101 gctttttcta acatatgttc aacttctttt atcatatacg ccttataccc cattgcaggt
    23161 attttatctt taaagtttat tgtatatacc ataaatgaat catagtttcc ataatgaaca
    23221 atttgacggt ctattaatcc tgcatctatt acttctttat gtaccacttc aaaatccaac
    23281 ttattattat ctttatcaaa tatttcaaat gctttcattt tagttataac ttgggcgctc
    23341 actatttcat tcctatcgta aggcattaag ttaaatgcta ttaacttatc taaagccatt
    23401 tcacaactca ttgcatcaac tattcttctt ttgtaaaatg ttattaattg gtctactttt
    23461 tcctcagcta agaaaaatct attcattatt tcctggtgaa ctttatctga acaacaacat
    23521 ccaatgctgt catgagcatg atttttcatt atttctttcc aaattaactc tattaatcca
    23581 tgatgatatt caaatcctag agaatatgca atagaagcta gtggctccaa tatgttagtt
    23641 atctttgatt ctattctagc atttgctgat tttatatcca ttcttgttga gaatatactt
    23701 ctatgaaccc tcatatattt cccatctaaa aattcccctt ttattgtatc tatatcctca
    23761 tttttttcta gctctttaaa tatgttttca tatctactta agaaaaattt tctgtctgga
    23821 taacattctt ttaatttata tataacttca aatatattct tttgaattgg catttggtca
    23881 tgtccatttg gaagtattat atggtcagta gttgcaccct tatcaagaac cggtaaatat
    23941 ttatccattc ttgttcttag ctcatcttca ttcattggta gatattttcc tattgcataa
    24001 cctaatggaa gtatttgaac caaaacagaa gacccatcat cactcttcca attaaactct
    24061 gttttattag ttccttttct ttcagaacat cctctccaaa acattgaata ttttatatca
    24121 aacccattta gtatttgtgg catttgagca ctttgaccaa atgaatctgg taaatatcct
    24181 attttcatat attctccaaa ttcatcacaa tcttttattc catatagtaa atttctaagt
    24241 atggattctc caccaactac catttcatca gtttgagtat accaaggacc tattattagc
    24301 cttccttctt gaactaattt ttttattctc tccttacatt caggcttaac tgctaaataa
    24361 tcttctaata tagcagtttg accatccatc acataatatg gatagttatg attattttct
    24421 aacatatcca ttatttcttc catattattt acaagtaata ttctagattc ctcagttgag
    24481 aagtaccatt ctctatccca atgcatatga ggaactatgt gtacattttt tttcattgct
    24541 tatcctccaa aacttatctg ataatatttt ctgtctaatt aaaaatccaa taaaaatact
    24601 actgagttta caaaattttt taatatttat tttatcacta taattaaacc tttgatttaa
    24661 atatctaata acgtatcttt taatgtatct ttctttgatt tttttgtttc ctagttatta
    24721 ttaataatat tgctgatatt atagctccaa gtactgccgc aaagaaccat attgatgcta
    24781 taataaatcc tgacatactt ccctctaaaa gcgccaatga gaatatcccc gctccaggca
    24841 cttgaagacc tatattgaag aatgctacta tagacccagt tatggctgaa ccagctacca
    24901 atgatggtat aacacggatt gggtcctcta tcataaatgg tattgcacct tcagttattc
    24961 cagccaatcc taatatccaa gtttgctttc ctatttcctt ttctgattct gaaaataaat
    25021 ctctgcctaa aacagtagct attgtaactc caaaagctga aaccatctta actgatgcaa
    25081 atattgcata aggtataaaa tttccacttg ctattgctcc tattgcaaat gtataggctg
    25141 ctttatttat tggtccacct aagtcaaatg aaaccatagc tcctattatt gcacctagta
    25201 gtattgcatt tactccagtc atcccattta accaatttat aagaccattg ttaagtgctg
    25261 ctacaggctt tcctactaca aataacatca ataaacctac taccaaagaa ccaactactg
    25321 gatataccca aaaacttacg aatccagcta atgtgccttt ggctggtata ttcttcttta
    25381 aaaattttac tatataacca gcaagtaaac ctcctaacat acctccaaga aatccactac
    25441 ctattaaatc agctgctaga cctgctgcaa atcctggtcc aagacctggt ttttcagcta
    25501 gtgagtaaga catatatgct gataatactg gaaccatcaa tgtacctaac atacctccag
    25561 aaagctttct taattgccat aaccaagaac cttcttgatt gtatagttct tgtagaccaa
    25621 atgcttgaga tgctaatact gcaaatgcaa gtatcatacc tcctgccaca ataagtggta
    25681 ttatatgaga tactcctgta agtattgctg attttacatc ttttttccat gatgattttt
    25741 cacaatatga tatatcagat gatttttcta tataatcacc tttaccttgt ttttgtgcta
    25801 cttctaaagc ttcgtatatt atttcttttg ctctttttag tggagctgct actgacgttt
    25861 ttatgtttgg caaatgttca aatctttctg tatttttagg tgctacatct gttgcatata
    25921 ttattgcatc tgcccttttt attatatctg gcgtatgagg gtccaccata ccattagctc
    25981 cttgcttttc tacatacaca tccacaccta tttctttacc agctttaatt aatgcttctg
    26041 ctgacatata tgtatgtgct ataccggcag ggcatgctgt tattgctaat ataatttttt
    26101 ttgattcatt ttgaatccta atctcttctt ttatttgttc tttcttttct tttatatcta
    26161 agttattaaa taactctttc ttagtttttg aatttaacaa catattcata taatcttcat
    26221 ttgacagtct agtaactaac tcagaaagta tttgtatgtg tgtatttcca ccttcacttt
    26281 ctggtactgc tattaaaaat attagctcta ctttattatt tgggtctacg ctttcccaat
    26341 tttctattgg tgtctctaag gtcatagctg caaatgcagc ttctttaact gacttagatt
    26401 ttccatgagg aattgcaagc cctctttcaa aacctgttgg agataaagtt tctctgtaca
    26461 ttacagcttc taaatattcc tcataagatg ttaacttgcc ttcatcatat agctttcttg
    26521 ctaagaactt tattgcgtct tcctttgttt ttattttttg ttttaaaaaa acaaggtttt
    26581 catttgtgat tttcttaaat tccaaaatta cgccctcctt aaatcattta atgctatgaa
    26641 tatagcataa aataaattat gaaaatgttt ttttgaaatt atacacacaa tttcttctta
    26701 aaaaataaag atttgtgtat ttatacacaa atctctatct taaccaatat tgctctatta
    26761 ttgctctcat aatcatcata attggaacct tatctgatat attgtaccca tttacttgtt
    26821 taattgttga atagcaatat aaacttatat ctgcaatttg ttgcaattga ttttcacata
    26881 tatgtgttaa agctactacc ttacaaccct tattaactgc tgtctttgct gcttctataa
    26941 cttctttcgt ttctccactt aaactaagtg caaaaaacat atcttgttca cctaattttc
    27001 ctatatcatg aagtacttca tgtctttgac tatagtaagt tatattatca tgcccacctc
    27061 taatttcttt tactaatatc tcacataaag gaatgttttg acctattcca taaactataa
    27121 tactcctaga tttttgaatt atatctgcta ctttatttat tttttcataa tctattagct
    27181 ctaatgtctt ttttatatct gattcaaaac cattttttgg ctctttatcc tcatttttta
    27241 actcttcttt taaattattt tttagttgag agaaaccatc atactccatt tttttacata
    27301 gcctaactat ggtatttggg acagtaaaaa atttatctgc taaagattgt attgataatt
    27361 ttactatttc ttctttgttt tttctcatat attcaattat catgtcttct gtatctgtaa
    27421 atttatactc atattttttt gctctttcat aaaattccat agtttctcct ccatctattt
    27481 tattattcat aagtaaaatt caaaaatatt agctaaaatt ttcgttaata taattataat
    27541 ttaatatttt taatttatta atgcatacta ctctttttat tatacataaa aaatgtgtac
    27601 ttcctatatt gatagagatg attcaagttt ttattttgat acatctctat caatattttt
    27661 ttagaaaata cacatttttt tcaaatactt atttttaatt aaaactttag gtaatctatt
    27721 ctttatatat aattattaga cttgaaaata tctttaaaat caaccaattc aatattattt
    27781 ctttttaacc acattttaac ttccttatca catagcattg catgttcata tactctctct
    27841 tgaataagag aagatgattt tataagacta taatctataa atcctggatg agtaaccatc
    27901 aatgttatat ctttatctaa atttatatat ttttcttcaa ataatttaac aaaagattta
    27961 tttaacttgt aaaaatcatt ctgaggatat ttacttggca tgctaataca tgaatccaaa
    28021 taatagggac aacttggtat accgtattct tttgcagcat cacacattgc tgatattgat
    28081 acatcttctt ctataccatg tatatcaata tactcaggca atttactcat atacaacttg
    28141 taagtttcaa tttgagcaca aacttcttca tatacttctt cataattaaa aacctcttta
    28201 cctgtcagta gttgcgttct tcttattttg ctagaaacaa attttccacc ttcatctaac
    28261 aaattcttta tcttaatatg ttctttgcaa caaggttctc ctattactaa attaacatgt
    28321 aaaccaaaac atacatcatc attttttacc atttctatag cttgtcttga aaaaggcata
    28381 ttcaccatga ttccagcact tgtaacaata ccgttcttat aagaagatat tatccctagt
    28441 gtaacacctt ctgaatatcc tatatcatct gccctaacta ctaatttctt cattttatgc
    28501 atctgcctgc tttctcaact catcaagttg catttccatc tcatccttta ctttaacaac
    28561 atccgttcct atgatgattt gaactgatgt attactcaat ttaattaccc ctttggctcc
    28621 tgttttttta atattatcat cattcaaaat agtggcatcc ttcacttcta aacgtaatct
    28681 cgtcatacaa ttattaagag tcactatatt ttcataacca ccacaatttt taattatctg
    28741 tttagccata tatgaaaaat taccatgaga aagttttaaa ttcttttctt tttcactaac
    28801 tttatcacct atctctatct caacttcacg tcctggtgtt tgcacattat cttttattat
    28861 tatatattta aatgttacat aatataatac aaagaataca actcctacag gtataactaa
    28921 ccacgcttta tctccataac taaaacttaa gacatagtca attatacttg ctccattaaa
    28981 tgaacccaag cgagcaccca ataaatatag tacaactcca cctagaccag aataaaaaga
    29041 atgaattata tataactttg gagatgcaaa taaaaacgaa aattctattg gttcagttac
    29101 tgttgccata aaagaagtca atgctccagc ggtcattgca ccttttactt cttgcttctt
    29161 tttatcttgt gctgttttat atatagcata agaaatagca ggtattgaaa acatcataat
    29221 tatgtaaaat cctgtccaga agaatcctgc atttgggtct cctgccataa atctaggaat
    29281 ttctcctctt ataacttctc ctgttgatgt tgtatattca cccatttcaa ataatatgta
    29341 agcatttaaa acttgatgta acccagttgg tattagtgca cggttacaga ataaaaacaa
    29401 gaatattcct attgcaccag ctcctcccaa ccaaaaacca aattgattca ttgcagcttg
    29461 tggatatatc cataataaac taaatatata tgataaaatt aaagcaacca ctggtgatag
    29521 tgtaattaca catttttctc ctgcaaagaa gcttaaaaat gctggaactt taactttata
    29581 tgttttattg taagtccatg ctatacaagc tccaacaatt atcccaccaa atacacctgc
    29641 atttaatgta tcattcaata actctaaatt tgacttaaat atcatatatg caactacggc
    29701 tgcaaccatt acacttgtct tgtctttaga ctttgagtag gcactagctg ctcctaataa
    29761 aaataagtag tccacatttg aaaatactat attagatata ttagatatag ctggcacatt
    29821 gagcatattt ggaccaaata ataatcctaa tatacctgct gctggcatta cagctattgg
    29881 caccagcata gtctttccta aaagtgacag ttgttgcatc attttgttta agaatctctt
    29941 catatccaat cccctttcta tttcggttta attttttgtg aaaacgattt attcacattt
    30001 tatattttaa atattatatt tgtttaaaca aattgtcaat attttttatt cattttattt
    30061 ttatcattta gtttaacata taaatccttt taagtgtatt gctatatcta gtctattttt
    30121 ttaattcatt ttttttattt taaaaatatg tttaaccatg tatttgaaac aacccacaaa
    30181 ataattctat tttgtccttt ttatggttga tatgattcta ttttcactat acatgtctaa
    30241 acacatttga tataatttgt tcatatcagt tcaataatat tactaaagac ctttaaggag
    30301 ggataggggt atatggctaa aaataaattt tcagagatat atgaagtatt gaaagaggaa
    30361 atacttgatg gtaaatatac aagtaatatg atgctgccta ctgaacttca acttattgaa
    30421 aggttttcat gtagcagaaa cactgtacgt agagcaattt ctcaattaaa cacagaagga
    30481 tatgttcaaa gtataaaagg caagggtgtt gtagtgttag aaaatagttg ttctaatgat
    30541 ttttttctaa atatgcacaa tttcaaaggt gtagaatcga ttgtagaaga taaaaaggtt
    30601 aatactgcta ctagtgttct ccacttcagc aaaatcttaa ttgataataa gttatctaaa
    30661 aagacaggtt ttaaagttgg tagtgaagta tattaccttc acagactaag atatattgat
    30721 aacataccta aaattctcga cattaactat tttctatgta gtatagttaa agaccttgat
    30781 gtttctatag cacaaggctc tatttataaa tatatagaag agtgtctagg tacaaaaatt
    30841 gtttcatcaa gaaagatatt taaaattgaa aaagctacag aacttgagtt aaaaacgcta
    30901 cctctaaatg attataattg tgttggtgtt attaaaaata gtgtatatac tgatgatgga
    30961 aagctttttg aatatactga atcaaagcat acacctgaaa catttgtatt catggatgtt
    31021 actcaaagat attaaaaaaa ttatgtttaa ttaaacataa tttttttacg tttagacact
    31081 ttatcccata ctaacctcac tattctatat acaattatat gtttatacaa ataattattt
    31141 attgtatatt ttagctttaa tattagttta acgcttgaca atttgtttaa acatattata
    31201 attaacttat aaattacagg aggtatcaag tatgaaaaat tggtggaaaa aagcaactgt
    31261 ctatcaaata tatccaaaaa gtttcaaaga tagcaacaat gatggtatag gtgatataaa
    31321 tggtattata gaaaaattag attatctgta ttctttaggt gttgatttat tatggttgac
    31381 tcctatgtac gtttctcctc aaagagataa tggatatgat atagaagatt attataatat
    31441 agacccaaaa tatgggacaa tgagtgactt tgaaaaactc ttaaaagaag ctcataaaag
    31501 agacataaaa ataatgatgg atatggtcct aaaccataca tctactgaac ataaatggtt
    31561 taaagaatct aaaaaaagca aagacaatcc atatcgtgat tattatttct ggaaagatgc
    31621 taaacctgat ggttctgttc ctaataactg gatatccaga tttagtggaa ctgcttggaa
    31681 atatgatgaa accactaacc aatattattt acatttattt gaagaaactc aagctgattt
    31741 aaattgggaa aatgaaaagg ttcgtgaaga atgttacaaa atactagaat tttgggctga
    31801 taaaggtatt gatggttttc gtttagatgt agtcaaccta ctctccaaaa cacctggatt
    31861 acctgatgac cctataacag gaccaaaagg agatggaaga actcactacg ctgatggtcc
    31921 tcgtatacac gaatatttac ataatatgaa tcaaaaagtt ttcaaaccta aaaatatagt
    31981 tactgtaggt gaaatgtcat ctactacacc tgaagagtgt atcaactata ctagagaaaa
    32041 tcgagaagaa ctatctatgg tttttagctt ccatcatatg aaaacagatt ataaaggagg
    32101 cgcaaaatgg actaatgaaa tgttctctct ggataaatta aaaaaagcac aatctgactt
    32161 tcaatataaa atgtatgaag gaaaaggatg gaatgctcta ttttactcta atcacgacca
    32221 acctagagct ttatctagat ttggagatga tagatttttt cgtcaagaaa gtgctaagat
    32281 gttagccata acattatttg gtctacaggg tactacatac atatatcaag gtgaagaaat
    32341 aggtatgaca aatgcctact ttactaaaat agatgaatat gatgatgctg aaagtaagaa
    32401 tgcatactat catatgatta aatctggtat tgatgaaaag gaggcattgc ttatacttca
    32461 acaaaaatcc agagataatg cccgtactcc tatgcaatgg gataatacta tttataaggg
    32521 gttttctaat cataaaccgt ggttaaaagt taataatgac aatattagtg tagaaaatga
    32581 gcttaatgat tctcgttcag tattttatac ttatcaaaga ttaatcaaat atcgtaaaca
    32641 atatgatatt tttacagaag gtacttatag attgttagat gaaaatcata aaaatctatt
    32701 tgtttatgaa agagagtata aaaaccaaaa tcttcttgtt ataagtaatt ttactcatga
    32761 taatgtagta tataaattac ctgaaactta tttgaataaa ttaaactaca caatacttga
    32821 aaccaattac actagagata taattgaaaa taaaatggaa ataaaacctt acgaaagtat
    32881 aatgatatat tataaataaa gttatttatg taaaaatcgg tataacaccc tggtgttata
    32941 ccaattttac taaattcaaa gtaatattta tagaatatct atagattaca ataattattt
    33001 gacttctaca tcattatatt tctttgaata tactttagtc atatagaaat atataggaac
    33061 tacaattatc atcccaccta atccccaaac tatcttcata ggttcatcaa tacctgcttt
    33121 tatcagtaat aatatactta taataactgc aaatattggt ataattgctc ctccaggtat
    33181 tctaaaattt acatctttac cagcatactt ctttctcaat attagtacag ctaaacatgt
    33241 aggaatatat tgaataaatc tagatacaac acttatttga gataatgttg tgaatgaacc
    33301 cgaccatgct attagtaaag ttcctatgac agatactata attgctatat aaggagctcc
    33361 atttttatta gtttttctta tagacttagg cattaatcct tgttcaacca aagcagcagc
    33421 agaccttggt gtaaatatgg atgatgcaat atttatacct ccaacagata ctatagttcc
    33481 tattgtaatt atatacccac ctataggacc taacatctta atggcagcat ctgcaacagg
    33541 agtagctgac cctgctaagc cattacccaa cacccctata cttattccca gtataagcat
    33601 atatattact gatactacaa acattaccat taccaatgct ttaggtacat ttttcttagg
    33661 gttctccata tcttttgctg caacagctaa agattcaaat cctgtaaaag cataaaataa
    33721 agttatagat actgctacaa aagctgatga cgcactttga cctttttgta ttattacaaa
    33781 tggggtaaaa ttagaaccct ttataaagaa taatcctaca gctataaata aaactattgg
    33841 taataatttt cctatagtca tgatattatt taagtgctta gattgtttca tccccataaa
    33901 atttataatc ccaagtccta caactaatat accaattatt atatttttag taagtggttc
    33961 tgctgcttgt ggccatacag atgatagtgt tacagcaaag aaatttgcca tagtagccca
    34021 agcaatcata ctaactatcc acttcataaa tccaacttca aatccaacaa aatctccaaa
    34081 tgcctctttt gcataaagat agggccctcc atcagtatca aaccaactac ctgcttcagc
    34141 aaaacataaa gcaatagaaa cagctaaaag catacaaaaa actaatacta atatacttgc
    34201 tggacctata tctgtataag catcaccagg taacccgaat atacctgacc ctattattgc
    34261 attgattcct aatagtacaa tactccaaaa acctaactta atgccttttt ccattgttat
    34321 tccccccaaa gccaacttaa tatatttgta ttttttattt agatgcttct ttaactattg
    34381 ccataaatat ttttttcata ttatcataat ccctagtcat catttctgga tgccattgta
    34441 tccctattac aaactcactt ccttcctttt ctatagcttc aattaaaccg tctttagatg
    34501 tagctgaaac aatatatccc ggtgctactt tgttgacaac taagtgatga aaactattaa
    34561 ctaaaacttc tttttctcct aatatttcat ataattttgt cccctccttt atctgtactg
    34621 tatgtgtagg tacattagat agatgttgct gattgtgttt aatataagca ccttcaatta
    34681 aagataaatc ttgatacaaa ctacctcctt cagttacatt tataatttgt tctcctctac
    34741 atatacctaa aactggtttt ttcatatcta aagcatgttt taataatttc atatcaaaac
    34801 tatctctctt tggtgatatt gcccccaact tattatgagg ttcttctttc catatgagtg
    34861 gatttacatc ttgtccccca gatagtataa gcgcatctat attagatacc tgctccttta
    34921 taatctcatc atcatataca atcggtacaa tatatggtat agctttacac ataactactg
    34981 attgtatata atcattattt acataagccc tttcatagcc aggaaacatt cctccttcat
    35041 caattagtaa attacctgat attcctatta ttggatattt ctttttagcc aatttcttca
    35101 ccccttaact tatttgtgaa caataaaaaa tgctacttct cttaaagagc aagtagcata
    35161 ccaagatttt atttatttga acattataat ttattttaga aatacttttt ttgaatacga
    35221 gtaatataaa taactttatt tacttatatt ttattttaat cctgtttagt ttgacttttt
    35281 taaattgttt taagtacaac taataggtaa aataggcatt tagattttcc tttttatcaa
    35341 cctattaaat actgtttaat atattttttc cccttctatt taattcactt ccctttctac
    35401 cagattcaca tattatcaaa tccagctctt ttaacatatt aagtattccc cttacctcac
    35461 cttctgttat attgtagcct aattcattta ttgaattaac aataccattt ctacctatat
    35521 ttttattaag attattggca ttttttaaac actccattac taactttact ttctctatag
    35581 atgttctatc ctttaatata cttatttctt tactaaaatc ttcagttata tttaataagt
    35641 tatgaggtaa atcatttatt aaaataacat cctcagaact cataatgtta atatacatag
    35701 cagtattttt taattctctt atattacctg gccattcata tgcattaata atgtcttgaa
    35761 catcttgact aattgctctc ttccttttca taaaatgctt taacataact tctatatctt
    35821 cttttcgctc tcttaaaggt ggtatattta tgggcaatac atttatccta tagtataaat
    35881 ctgctctaaa tctatctcct tttatcattt ctaataaatt tttatttgta gccgcaatta
    35941 ctctaacatc tatatctatt acattttctc ctccaaccct cattacttga ttctcttgta
    36001 atactctaag taatttgctt tgtaagtata taggcatgtc tcctatttca tccaaaaata
    36061 tagttccatt gttagcctgt tcaaataatc cttttttacc tccttttaaa gctcctgtaa
    36121 aagcaccttc ttcatatcca aaaagttcac tttcaagtaa attttctggc atagctgcac
    36181 agtttatcgc tataaatggc tgactatgtc ttggtgatgc attatgaata gactgagcca
    36241 tgagttcttt tcccgttcca ctttctcccg ttataagaat tgtataatca gattcagcta
    36301 ttttcatacc taatttctta gttctttcca tcttcgttga aactgttttt atatcatcaa
    36361 aagtatatct agcaacttgt cccttttctc taagcttctt tgataggttt tgctccaatt
    36421 ttcttatgta agttatttct tgaatacaaa aataatatcc agtaaccctt ccaaaactct
    36481 ctatattata cttatttacg tttaagtact tattattaaa ttttataact tcatcatgtg
    36541 ccttactcaa actaaaaatc tttttaatat tctcattctc taattcatct atatttttcc
    36601 ctataatatt actttgaata tctagtatat cccctaaact attattgcaa ataattatgt
    36661 ttcccttatc tgataccatt gccataccta agtttgataa atttattata gaatttaatt
    36721 catctgcttt tatagtaagt tctttatatt tagtatttat tcctgaatat aaactaatta
    36781 tttcatctgt atacttaatt agcctttcag taatttcttt atcatccagt ttgagttttg
    36841 ctattatttg aataaacgtt gaactatcaa tatacctatg gcctaaatca ataacttctt
    36901 ctatatattt tggaactaaa cttgcctctc ctggcgttat agctatctta atatttctat
    36961 attcttcatt gggattgtat ggtataaaat tcagatgatt tattcctata ttgtataaat
    37021 tagatattgt ttccaataca gtatgcttat tatcgtttac tactaataca tctattcctt
    37081 ctggcaatga aaataactta tatatatcct tttgctttat gctcctattt atagttatta
    37141 tctttgagac atcttctaca tattttttta tttttaatac tctatcatct atcattacca
    37201 atattatgtc atcttttatt aattccttct ctgttatttt atttaaataa taatttttta
    37261 tcattacttt atctttaaat atcatttcta gatttttatt taagaaacat tctaatttag
    37321 acaacccatc agttactact gctattgttt tcatttgatt actcctttta aaatatactc
    37381 tactctttta taatatacat ctgattttca ctttaacagc acaaaaaatt gtacatttag
    37441 tatactaaca tattccttta ttagttttat gtccctaaat atcatagttg caatatctat
    37501 catatttctt aaacttctat aaagcaaaaa ttgtgccaaa ttaataattt tcttaatttt
    37561 attctaaagt tcctcaatag tatttacaga cttagcactt atagcttata agtctttata
    37621 ttattaatct atttttatag gttaattgta caaatcatat atcactttta cccattaaat
    37681 gcattataaa aaagatggaa taacgattcg tcactccacc ttttttgtaa taaaaaattg
    37741 tatatataaa cactatttac tactcttcta agtacaaaca ttatctacat actataacct
    37801 ttcaccatta gattctatta cttgtttata ccagtcaaaa ctctttttct taatcctaga
    37861 caagtctcca cttccatcat catacttatc cacgtatata aacccatatc tcttggccat
    37921 ctcaccagta gatgcactta ctaaatctat acatccccac ggagtatatc caaataaatc
    37981 aacaccatct tctacagctt ccttcatttg ctctatatgt gcttttaaat actctatacg
    38041 atagtcatca tttatcactc cattagattc ttttttatca taagctccta aaccattttc
    38101 aactaccatc ataggtattc tatatctatc ataaatttca tttagtgcat atctaagccc
    38161 ttcagggtct atttgccatc cccattcact agacttaaga tatggatttt tcactccacc
    38221 taatatgttg ccacttgtat tatcttcttt agaatcacta cttacacatg tagacatata
    38281 ataactaaat gtataaaaat ctacacaacc ttcttttaaa atttctaaat ctcctttttc
    38341 catttttatt tcaatattat tttctccaaa atatctattc atataagttg gatattcgcc
    38401 acgcacttgt acatctccac agaaataatt cattactcta ctatctcttt gagttttaac
    38461 tgcatcatct ggattacaag tataaggata cattgttgca aatatttgca tacatccaac
    38521 ttgataattt gaatctattt catgagctaa cttaacagcc ttagcacttg ctacaaattg
    38581 atgatgtaac ccttgaaaac gaagttgagg tatatctact tggtttataa aatctgtagt
    38641 cccttcattt aatattcctt gtgataagta gcctcccata ggcattgtag ctgaatttat
    38701 ttcattaaat gtcagccaat attttacttt tcctttatat cttttaaata ttgtttcaca
    38761 gtaattcata tacaagtcta ttacatctct tgaaacccat ccattgtatt tctctgttat
    38821 tgcaaatggt atttcatagt gagatattgt tattaatggt tctatattat attttaaaca
    38881 ttcatctatc acattttcat aaaatttaag tcctgcttca tttggttctt tctccattcc
    38941 agttggaaat attctagtcc atgctattga aaatctaaat accttaaatc ccatttctgc
    39001 aaataaagct atatcttctt tgtatctgtg ataaaaatct attgcttcat gacttggata
    39061 aaatgttcct ttttctataa ctctcgttat tcttttactt actttttgag aacctccagt
    39121 acacatatct gaacaagaag gacctttccc atctaaattc catgctcctt cacattgatt
    39181 tgctgctgtt gctccacccc ataaaaaatc ttttctaagt gccataattc tacctccaat
    39241 aatttattta tagtaacttt attttaaaat aaaaatacat atatataatt cttaattaat
    39301 agaactctct tttacgctaa aacagttatc aattcctgac tattatttac atgcttttgt
    39361 gtattttcta aaatatccac ataatcagaa taattagtaa caactactgg tgtttctaag
    39421 caatatcctt cttttgaaat actttctatg tcaaaagtta ttaatatctg acctttttta
    39481 atcttatccc cttgattaac aaaagatttg aaatatttac cttccaactg aatagtattt
    39541 aaccctatat gtatcaatag ttcacaacca ttatcagata taattcctat tgcatgtttt
    39601 gttggaaata aagtcattac agtcccatca aatggtgcca ctacttttcc ttctttaggt
    39661 gttattgcta caccttttcc caatatacct gttgaaaacg ctgcatcttg tatctgctcc
    39721 aaagccttta cttcaccttt tattgggcta tatactactt ctcttttgtt tttttctata
    39781 acgccttgcc cattatcatc tttagaaatt tctactatag catcatcttt ccaaaaaaat
    39841 aatgttaaaa caaatcctac tacaatagct actattattg ctattatagt tgcataaaca
    39901 cctttcatat ctccagttgt tggatttata taattaggta taccaaatat tccaagtcca
    39961 cccattacat aactttttac attcattatc cccattacag ctcctgccac accaccacct
    40021 acacagctat atatgaatgg tgtttttctt ggtaaagtta ttccatatat tgcaggctct
    40081 gttacaccac atatccctga aataactgca ggtatacaca gctcttttat cttcttatct
    40141 tttaatttaa agtacattgc tgctactact gctgtttgtg caaaagatgg tacaaatgct
    40201 cccactaata ttgtgtcaaa ccccatagtc ataaggtttg acatagctat tggtactaat
    40261 ccccaatgta gaccaaatat tacaagcact tgccacaata aaccagttaa tgcaccacat
    40321 aaaattggtg aaaagttaaa taaagctaaa aatccatttc caactatatt agaagcatag
    40381 gtagctatag gccctataac cataaatccc aatggtaaag aaactaataa aactgccatt
    40441 ggtacaaaga aattctttac tacatctggt attattttag caaacacttt ctccaactta
    40501 gctgctaata atactattaa aattactggt actactgttg aagtgtagtt cattgctatt
    40561 actggtaatc ctaagaaagt tatatatact ggtgattcaa atattgttcc agtaaataga
    40621 ttgtataaag gttgacctgt agatgctaga gtatctaatt gaatacctga atagcataaa
    40681 actgcaccta taagcattcc aataaatggt tttaatttga atttcttagc agctgtatat
    40741 ccaagaatta ttggcatata ctggaacatt gcatctccaa ttgcatttaa cataatatat
    40801 gcaccatcag tttctccata caaacccaat gctgtaaata ctgcattaaa tccttttatc
    40861 ataccagcag cacacagaac tcctaatatt ggttgaaaaa ctcctgatat tatgtctata
    40921 aacttattaa gtaatccttt tggcgcatct gagtctgaat tatctattgt atctcctcct
    40981 aatccagcca aatcgcacac aacttcataa acatctggaa catgattacc aataactact
    41041 tggtattgac ctgcactttt cattacagta actataccat ccattttttt tagcacatca
    41101 tcatttgcta ttgactcatc ttttaattta aaacgtagcc tcgttataca gtgagataaa
    41161 ctgctaatat tatcctttcc tcctacattc gcaatgattt cttttgatag ttgttcatac
    41221 tttctcattt tttctcctcc attttctttt tgaaatatga aggtttggcc aacttaatgt
    41281 caccacccaa tatatataaa cctaattatt aggatttata tctgacttaa tacttttact
    41341 taccactctt tcaacgtgaa tagttaagta taatttttcc tcatctgtta aatcgtagtt
    41401 gtatttttta tgtataaatt catccaactt ttctatgaat ttataagcat ttttatattt
    41461 aacttttatt acatttaata aatcatcttc attattttca tgtaaattat tactaacaat
    41521 tcgttctgca aaatacttta agtgagtaat aaatctatga taataaactg aatcttcatc
    41581 aaatactatt ctgtaatggt atttaaccat atttgaaatt tcttgtatta tttgtgtaat
    41641 ctcatatata gtttttatac tttgatccat ttgtgcatta actatatgca gtgctataaa
    41701 tccagcttca tcttctgata gtctaatttc aaattttttt tctatataat ccaatgcctc
    41761 taaccctatt ttaaattcac tcttataaaa tctttttata tcccaaagta atacattttt
    41821 tacacttact ccttcacgag ctctttctat tgcagtatac atatggtctg ttaaagaaat
    41881 ataaatacta tcatttaatc ttttccctag tttttcttta gcatataaaa ttatttcatc
    41941 tgataattcc atatatctta taggaatatc tgctattagt tctttaaatt tatcagatat
    42001 atttgggtct gaaagtttga atatcttatc aattttatcc tttgatatat gctctcctgt
    42061 acgtttttta taagctaaac ctctccccat aactactatt tccttatgat tttcatcagt
    42121 tgtaataatt acattattat tgagtatttt ttcaataatc attgtgatgc cccctttatt
    42181 agcattaagt taaaataaat cttttttaat tatttagaat atttaaaatg taatttatta
    42241 cataaaaaaa aacctattta ttacacatta aatagactcc catgagtcct gagtaataaa
    42301 taggtttagc ttgtatttaa acaatcacta tccgtttgta ttttatttta attattattt
    42361 attatatgtt agcaaattta ttttttagtg tcaacttttt ctcaatattt taattttaaa
    42421 taaattatat aaattgaatc ttcaaagtac tatttttatc aataaacaaa ataaaaaagg
    42481 tattactttc tttaagtaat acccatttat ttttaattta agtggttttt cttaatctca
    42541 tcaagtagtc catcttctaa ataaatttct aatgcacatc ctaccattgc ctttactgct
    42601 ttgtgaagtt tatcataagc ttcctcagaa tttgcataat ttagaaattc tttttcatga
    42661 acatatacca ttggacttat atctaaggct atttctgtat acatagtagg gcaaacttga
    42721 cttacattac ctatatctgt agaacctact ggaccatctt taccttctaa tatatctgtt
    42781 atacctgcct caatcaaatt attctttgtt atcttttgaa gtactttgtt atttattatg
    42841 ttgtcaaaag aattttcaaa gtatctataa gaaagcttag ctcctgtcat taactctgca
    42901 ccttttgcac agtttataac ttttttagtt acttcatcta aataacttct ttcagcagct
    42961 cttacgaaaa acttacaagc tgctttttct ggaactatat ttggagcctc tcctccattt
    43021 gaaactatac catgtattct aacatctgat tttacatgtt gtcttagtgc atttatccca
    43081 gcaaaagtaa gttgtactgc atctagtgca ttgactccca tatgagggtg actagctgca
    43141 tgtgctgata ccccttcaaa actaaactct aatgaatcta tcgccaaagc cttacagttt
    43201 atattattaa aagcttctat atgcatttga taacttacat caacatcatc aaaagctcct
    43261 gccttaacta aatcacattt tcctcctgtt gtttcttcag ctggtgttcc tattagtaca
    43321 atttttcctt taaaattttc ttttaactta cttaatacta tacaagctcc tagtgtagat
    43381 gctgcaatcc agttatgtcc acaagcatga gctggttctt tattttctcc atatccagga
    43441 agtgcatcat actcagctaa aaatgctatg gtaggtccat cattatctcc taattcagct
    43501 ctaaaagcag tctccatatt acaatatgga taaactgtat caaatccata ttccttcatt
    43561 ttttctacta agtattttga tgatatgtat tcttcttccc ctaattcagg attgttaaat
    43621 ataaaattac aaacttcttc aatttctttt ccaagttcat tctctatctc ataagctctc
    43681 ttctttatac tctctatcat aatttacctc ctactagaat cctagatata atcctattgt
    43741 caaaaatgcc attacaagaa ttgttaatac taccattaaa ggaattacga atctaaccca
    43801 ctttgaccaa ggaatattgg ctactgctag tgctcccatt aaaacacctg atgtaggtgt
    43861 tatcaagtta gttacaccta taccaaattg ataagctgta actatcattt gtcttgatac
    43921 atctactaag tcgccaagtg gtgcaagtac aggtattgta agtgatgcaa gccctgatgc
    43981 tgatggtatt aaaaatgcta ttaatccttc tattaagaaa gttaaattta taaatatagt
    44041 ttttggtaat ccatttaata aatttgctgc agaatttaaa attgtatcta ttataaatcc
    44101 attttctgct attattacga ttccacgtgc aaatgctata cacaatcctg ctgtaattaa
    44161 atctcctgct ccacttataa atgaattaac tatctcatct tgtttaagcc ctgatgctat
    44221 tcctgatatt atacctatag ctgtaaatat cattgcaatt tcagttatgt accattctag
    44281 tcttaaaact ccatatatca taatcgccat acctattgtg aaaatggcta ttaccatggc
    44341 ttctcttttt gtaaattcct ttatatttgt atcactatta accattaatt cttgatttag
    44401 tgatatatca tgtactaaag attttttagg gtctttttta actttattgg catacatcat
    44461 aacaaatcct atagatatag ccatatatat tataaacata acaactctaa actctattcc
    44521 agaacctggt actatttgtg ataatgcttg tgctattcct acagagaaag gattcgtagt
    44581 agctgctcca aatccagctg ttgctcctat aaatactgtg gccactgctg tcaatgaatc
    44641 atatcccata ctggtcataa gtggaataaa tatcatataa aaaggtagtg tctcttcact
    44701 cattccaaac gtcgtcccac ctagtccaaa tagaatcatt gaaatcggta ttattaaaaa
    44761 ttgaaacttc ttcattttat ttacagtatg agctataaca gcttctatag ctcctgtttt
    44821 attaactatc ccaaaagctc ctccaactat taaaacaaat ccaactactt cagctgcatc
    44881 tataaacccg tttataggtg ctgtaaatac atcagtaatt ccttgtggat ttgatggtgc
    44941 tttttcataa gttccagcta caacaactga ccttccgtct atatctactc tttcaaaatt
    45001 tccacttggc actatccaag taagaattgc cattaacact attagcaaaa atattattgt
    45061 aagtgtctgt ggcatagtaa actttttctt ttttaaactg ctcattttta atctccccac
    45121 tttttaaatt aaatatttta ttattgcatc cctttatcct attttgtata gatgtaaaaa
    45181 agctcaacat ggatacatgt tgagctaaat gtgctaaata caacatacct gcatcttccc
    45241 tttatcttgt ttaagatagc tctccatcaa aattcgggat aattttgatg acagtactgt
    45301 acctattaaa tacagtccca gcactagctt cggacactag cacttcggca cttactcctt
    45361 ttatattgcc tcattggttt ccaacctctt caatattaat cttagtaaat gcaacctcta
    45421 cctcaatctg tttgttagat gaggtaattc ttattaattt ttattaattc aaagtaattg
    45481 tagcataata tgtcgagatt gtaaatattt tcagtataat aattcttact ttatgatttc
    45541 attccatttt tcacaaataa ttttttctcc atataattta aatactgtag tatattttaa
    45601 atacacacaa actttccaca aattttaaat tattataata cctttataat aaaaaataaa
    45661 agctatgtaa acttatcaat ttacacagct ttatatacat taatctactc caagtacagc
    45721 tttaagcttt gatgctattg taataactcg tggaccaaat ataacttgaa ctgagttttt
    45781 ctttttaaca actccacttg cacccaattc atttacccag aagccatctt catagaatcc
    45841 atcaattgaa tttagctcta ccctaagtct agttgcacag tttgtaacat tttttatatt
    45901 attacttcct cctaaacctt ctattataga atgtacaagc tttgtatctt tatcatctac
    45961 ttcctcttct actgcattgt tgtatgctgt tttaccagca tctacagatt gtctttctct
    46021 atattctttc ttactataaa gcttaacttc ttctgaactt cttcctggtg tttttatatc
    46081 aaacttcttg attgcccatg taaatattat ataagtaact ataaaaaata ttggtatcaa
    46141 aaataataat ggtaacgcat ccactttttg tggttgtaat aaattaggaa acatatttct
    46201 aaatgattct cccattatag aaacattaaa catttctgta aatacatacg tcaatcctgc
    46261 cagtggtaca tatgctacaa aatacaataa tggtgcaaca aataggaatg tatactctat
    46321 tggttccgtt acccctacca acatggctgt tactattgct ggtatcaata tacttattgc
    46381 ttttttctta ttagcattat cagccaactt atacatagct aaagctgctg ctggtaatat
    46441 tgcaaaatgt attaataaac gacctgttgt gaagttacga gttatataag atagtgtatc
    46501 tgggtctccc atttgagcta atcttatatt attaacaccc tcgtaaattg ctccacctac
    46561 ttccattgtt ccacctactc ttgtatattc tatagggaat gcaattaagt gatgtatacc
    46621 aaatggtaat aatgctttat ccattgctcc aaaagtaaat gttcctaata atccactaga
    46681 acctatccaa ttaccaagtt tagataatgc caaccctact ggtggccata taaagtacat
    46741 tgctgtaccc aatactattg atgctataaa catcataatc attgaaaatc ttggacctga
    46801 gaaaaaagat aatgcactag gaagttctat gtcaacaaat ttgttatgta ctgcactagc
    46861 tacaaatcca gctattatag ctccaaatat actacaatca tatgaaaaga ttcctaagaa
    46921 agaaccatat aaagaacttc gactatatgc ttctgcctct cctaatccag atgccataaa
    46981 cgcttctgct gtaactgtat ctgaatttat accatttgct gctaacaatg tacttataac
    47041 tgtgtgcatt cctataaata aaacaattcc tcctaatgca ccccatcctt tttccttttt
    47101 agataatcca aaggctatac ctgtaacaaa gaatattggt aagtatctca ttaccataaa
    47161 tcctaaatcg tttattactt taaagaatac atttataaat gacccttctt ttattagtcc
    47221 gctaagccct aatgcagaaa cgttttccat atttgcaaaa gctgacccta atcctataaa
    47281 tactcctgct acaacaagta atataacagg tatcatcatt gctccagaaa gtacttgtaa
    47341 tttatccttc atagtattat ttcctccatt tttattgaaa tgccaggcta tataatgcat
    47401 atatatatac ttctattgct ttgtataatg tatctatact tatattttca tttgcctgat
    47461 gtgaatttgg attatccccc tctattgtgg gtccaaatgc aactccattt tttaacactc
    47521 ttgcataaga tgcagctcct tttgtaaaaa gttcggcttc ttttccaaaa ccttgtttat
    47581 atgcatttga taatttattt attaattcac tatctggttt tacatacagt aattttaaat
    47641 ccttatatat atcaactatt aaattttctt tttttccaat ttcattaatt ctattagtta
    47701 taaactccca acttataccc ttaggatatc taaagtctat tttcatattc aattcatttt
    47761 tctctaagaa gatactcatt atacatattg tagaaactcc catatctaca tcttctttat
    47821 acatccctaa tttttttcca tgattatcat ctgtaaagta tgaatttaaa atatccctta
    47881 ttataacgcc tttatcattt agttttttaa ttttctctaa atcttttgct aaactaaatg
    47941 cacagttata tgacctgtgt ggatttctcg ataaagccct ttctcctgta tatcttacta
    48001 atactttacc ttcatctaat tcttctatct ctgtataata agctaatgat tctactaaat
    48061 ctttcttatc attagtttta aaaacagcct caattttgtc acatacaaat ccatcttctt
    48121 tgtttgactt tatatcaact aaattgtgat ttctaaattt atctttatct attttctttc
    48181 ttagattgaa gtataaaata cctttttctc catttactat tgggaagttt ccatctggag
    48241 aaaatgcaaa ttttggctgt tcattgacac taaagtaatg ctccatacat tcccaagtcg
    48301 tctcttcagc tccacccact atcaatctaa tctttctctt cggcttttca ttaagttctc
    48361 ttaagaaaag taatgcatat aaaatcccta tcaaaggtcc tttgttgtca ttaactcctc
    48421 taccataaag atagttatct ttttgacata ctttaaaagg tttactcttc caaagttctt
    48481 tattatatat tggcaccaca tctatatgat taagcacccc tactacttct tctccttctc
    48541 catattctat atgaattgca tatccatcaa agtcttttac aacaaaatct ttgtcttttg
    48601 ctatttgaat aagcttatcg aaggcatttc ttatttctat cccaaatggt tgatttatat
    48661 ctgttgtact ttcatctcta attgaaggta tttcaactaa acttctaata tcatttatga
    48721 tattttcttg attggatttt aaataatcaa taattagttc tttgtttact atcatcttaa
    48781 ttatttatca cccctttata ttaattttac aaagcatttc agttcatatc tacattataa
    48841 attttgaata ttattttttc aatatttcaa atacttctct aagttaccat aatgatacgg
    48901 taaacaatca acagattatg acaaaatcat actataccta ttactaatat tttaacttat
    48961 ctattacgtc ttttttaatg taagtttatt ccaaaaatac ataaaaaaag atgtagcctt
    49021 agctacatct tcttatatct attcaacttc cattattatg ctattaatat ctacatttcc
    49081 aggttctatc ctatctttta ctgttctctt aacagaatca gcatcatcca ttataactac
    49141 aggagttatt atatttaaat catttgacgt tataaaatct aaatctactt ccattattaa
    49201 atctttagct tttaccttat ctcctgcctt taccttaaca tcaaacggct ttccttctag
    49261 tgacattgtc tctaaaccta catgtattaa tatatttgta ttatcattac tcttaatcat
    49321 tattgcatgc tttgtatcaa atacattgac tatttcacca tcaacaggag aatataactt
    49381 accttcactt ggtattattg ctactccatc tcccatcatc ttttgggcaa aaactgcatc
    49441 tggaacatct tctaagttca ctaaatttcc cttaaaaggg ctatatattt tctcaatctt
    49501 attttctttt ttcttaaata tattaaacat aacacactct ccacattata aaattttatg
    49561 ataatatttt tcttaaagct tttgctacac catcatttac attagtatca gttacaaaat
    49621 ctgccataga ttttacttca tctgtagcat ttcccatagc aataccaaga cctgcaaatt
    49681 taatcatgga tatatcattt tcattatcac ctatacaaat aacttctttg ctatctatac
    49741 ctaatatatc acaaaatttt ttaactgcaa taccttttga agtaccttta ctcataactt
    49801 caaagtttat ctttccagaa cttactactt ctaaatcttc ttgtttttta aattcttcct
    49861 ttgcttctct caaagaatct acgttttctg agaaaagtat acattttaaa atttcattat
    49921 cagctttttc taataaagtt tcacaacttg cattttctat tatttcaatt ctgtcttctt
    49981 ctttattttt actatttatt aatctatatg gatgttcttc acctatttca ttgttggata
    50041 taactatttt acagccttta aaatgtttgt ttaaattata cttttcacct attgtataga
    50101 ttttctttag agcttctttg ctcaaaacct tcttatactc atatcccagt aatttaaaat
    50161 atgtcccatt agttgatata atatatatat catcccctaa aacacttgta aaatatttca
    50221 tagcattata aggtcttccc gtaactaagg ctaccttgat acccttttcc atggcttctt
    50281 ttagtgcttt aatattttct tctgatactt caaatccttt acccatcaaa gtaccatcca
    50341 tatcagtaca aattaattta tatcgcattt gtatcacatc ctagtaagat ttttctaata
    50401 tttcaacaag gtcttctttc tttccaaaag gtaaaaatcc taaaactgga cagcttgtta
    50461 tttgttctaa ttgttccttt gttacattaa actcagacat ttttgtctta agccccactc
    50521 tctttaaaaa actttctatt tcatttccta aagttttagc tgcttcatca tcttccactt
    50581 tttctaattc ttggtttagt attctcgcta ctttggcaaa tttctctata ttatccttat
    50641 agaacttttc tgcaaatttt gggaacacta tagccaaagc ctcaccatga ggaatatgtg
    50701 taactcctcc tattatttca cttagtggat gtggtgctgc tgctccagca tttgccagtg
    50761 aacttcctcc taatgtatct gctactgcta actttgttct gtattctata ttatttttat
    50821 cttccattac tttaggtaga tagttaatga ctaattccat agatttttca gacattatct
    50881 ctgtaaaatc attagcattt ggatttatat aactttcaaa tgcatgagta aaagcatcaa
    50941 atgctgttga tgcagtaatt ctctctggca gagttaacat aagctctggg tcaactatac
    51001 attcttttga gaaacaattt tgatgaaata ttgtattttt ctcgcttcct ctcgatatta
    51061 ctgctgcttg tgtaacttgg cttcctgttc cagaagtagt tgatacagct attaatggta
    51121 attctttttc acttacctta tcgtatattt taaatggatt tgtatatgta ctaaacatat
    51181 aatcccaatc tattttttct aaaccaaaag tcaatgctaa tacttttgca gtatctatac
    51241 ttgaacctcc tccaacagct aaaacaacgt ctacagattc ttttttagct aattcaaacc
    51301 cttcgtttat taattctaca gttgggtttg gctctactct ctgaacctcc catgactaaa
    51361 gtcacagggt tcctgcttca cagaattttg catactaaaa tatgtcttac taattctcca
    51421 caggccatcc ccgtagttcc tacggttctt acataagtta tttattttga attgttatta
    51481 gtcttaatcc ttcttttaat atatttatac ttgcatttat atctctatcg tgagtttcat
    51541 tacaactagg acaaatccat tctcttatat ttaagtcctt cacttcctcg tttttatacc
    51601 cacatttatt gcatatttgt gaacttgcaa agaatttacc tacctttaca atttgtctac
    51661 catgccaatt agccttgtat tctaattgac gaataaattc tgaccatgat acatctgaaa
    51721 ttgagcgaga tagtttacga tttttaatca tattctttac ttgtaaatct tctatgcaaa
    51781 taatatcatt ctcttttatt aatttagtag ataatttttg cagaaaatca tttctttgat
    51841 tagctatatg ttcttgaagt cttgcaactt ttaatcttgc tttatttcta tttaaactac
    51901 cgattgtttt tctcgataat tctctttgta atttagcaag tttgtttaat gacttcttaa
    51961 gatacttagg attttct
//
LOCUS       DAYAOY010000017        50685 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYAOY010000017 DAYAOY010000000
VERSION     DAYAOY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.017"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(58..1461)
                     /locus_tag="ACGI6B_001074"
     CDS             complement(58..1461)
                     /locus_tag="ACGI6B_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9750538.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(1445..1972)
                     /locus_tag="ACGI6B_001075"
     CDS             complement(1445..1972)
                     /locus_tag="ACGI6B_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9750539.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            2252..2767
                     /locus_tag="ACGI6B_001076"
     CDS             2252..2767
                     /locus_tag="ACGI6B_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9750540.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            2816..3319
                     /locus_tag="ACGI6B_001077"
     CDS             2816..3319
                     /locus_tag="ACGI6B_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9750541.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            3316..3966
                     /locus_tag="ACGI6B_001078"
     CDS             3316..3966
                     /locus_tag="ACGI6B_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750542.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(4101..4880)
                     /locus_tag="ACGI6B_001079"
     CDS             complement(4101..4880)
                     /locus_tag="ACGI6B_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9750543.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(4992..5243)
                     /locus_tag="ACGI6B_001080"
     CDS             complement(4992..5243)
                     /locus_tag="ACGI6B_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFG9750544.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            complement(5572..6441)
                     /locus_tag="ACGI6B_001081"
     CDS             complement(5572..6441)
                     /locus_tag="ACGI6B_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750545.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            complement(6779..7540)
                     /locus_tag="ACGI6B_001082"
     CDS             complement(6779..7540)
                     /locus_tag="ACGI6B_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9750546.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            complement(7556..9091)
                     /locus_tag="ACGI6B_001083"
     CDS             complement(7556..9091)
                     /locus_tag="ACGI6B_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9750547.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            complement(9505..11745)
                     /locus_tag="ACGI6B_001084"
     CDS             complement(9505..11745)
                     /locus_tag="ACGI6B_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9750548.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            complement(12273..13073)
                     /locus_tag="ACGI6B_001085"
     CDS             complement(12273..13073)
                     /locus_tag="ACGI6B_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9750549.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            complement(13464..14339)
                     /gene="hslO"
                     /locus_tag="ACGI6B_001086"
     CDS             complement(13464..14339)
                     /gene="hslO"
                     /locus_tag="ACGI6B_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFG9750550.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            complement(14624..15370)
                     /locus_tag="ACGI6B_001087"
     CDS             complement(14624..15370)
                     /locus_tag="ACGI6B_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9750551.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            complement(15357..15602)
                     /locus_tag="ACGI6B_001088"
     CDS             complement(15357..15602)
                     /locus_tag="ACGI6B_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9750552.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            complement(15733..16851)
                     /locus_tag="ACGI6B_001089"
     CDS             complement(15733..16851)
                     /locus_tag="ACGI6B_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFG9750553.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            complement(17057..18250)
                     /locus_tag="ACGI6B_001090"
     CDS             complement(17057..18250)
                     /locus_tag="ACGI6B_001090"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFG9750554.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            18573..19454
                     /gene="dapA"
                     /locus_tag="ACGI6B_001091"
     CDS             18573..19454
                     /gene="dapA"
                     /locus_tag="ACGI6B_001091"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9750555.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     regulatory      19532..19706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            19879..20880
                     /locus_tag="ACGI6B_001092"
     CDS             19879..20880
                     /locus_tag="ACGI6B_001092"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFG9750556.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            20997..21884
                     /gene="dapA"
                     /locus_tag="ACGI6B_001093"
     CDS             20997..21884
                     /gene="dapA"
                     /locus_tag="ACGI6B_001093"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9750557.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            21951..22700
                     /gene="dapB"
                     /locus_tag="ACGI6B_001094"
     CDS             21951..22700
                     /gene="dapB"
                     /locus_tag="ACGI6B_001094"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFG9750558.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            22924..23640
                     /gene="dapD"
                     /locus_tag="ACGI6B_001095"
     CDS             22924..23640
                     /gene="dapD"
                     /locus_tag="ACGI6B_001095"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFG9750559.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(24092..25081)
                     /locus_tag="ACGI6B_001096"
     CDS             complement(24092..25081)
                     /locus_tag="ACGI6B_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFG9750560.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            25521..26276
                     /gene="dapB"
                     /locus_tag="ACGI6B_001097"
     CDS             25521..26276
                     /gene="dapB"
                     /locus_tag="ACGI6B_001097"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFG9750561.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(26783..28459)
                     /gene="bclA2"
                     /locus_tag="ACGI6B_001098"
     CDS             complement(26783..28459)
                     /gene="bclA2"
                     /locus_tag="ACGI6B_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HFG9750562.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(28837..29376)
                     /gene="hpt"
                     /locus_tag="ACGI6B_001099"
     CDS             complement(28837..29376)
                     /gene="hpt"
                     /locus_tag="ACGI6B_001099"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFG9750563.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(29704..31011)
                     /locus_tag="ACGI6B_001100"
     CDS             complement(29704..31011)
                     /locus_tag="ACGI6B_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HFG9750564.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(31436..33211)
                     /locus_tag="ACGI6B_001101"
     CDS             complement(31436..33211)
                     /locus_tag="ACGI6B_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750565.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(33208..33345)
                     /locus_tag="ACGI6B_001102"
     CDS             complement(33208..33345)
                     /locus_tag="ACGI6B_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750566.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(33423..33992)
                     /locus_tag="ACGI6B_001103"
     CDS             complement(33423..33992)
                     /locus_tag="ACGI6B_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFG9750567.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(34176..34811)
                     /locus_tag="ACGI6B_001104"
     CDS             complement(34176..34811)
                     /locus_tag="ACGI6B_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9750568.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(35479..36360)
                     /locus_tag="ACGI6B_001105"
     CDS             complement(35479..36360)
                     /locus_tag="ACGI6B_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFG9750569.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(36480..37487)
                     /locus_tag="ACGI6B_001106"
     CDS             complement(36480..37487)
                     /locus_tag="ACGI6B_001106"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HFG9750570.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(37507..37980)
                     /locus_tag="ACGI6B_001107"
     CDS             complement(37507..37980)
                     /locus_tag="ACGI6B_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9750571.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK"
     gene            complement(38007..38474)
                     /locus_tag="ACGI6B_001108"
     CDS             complement(38007..38474)
                     /locus_tag="ACGI6B_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9750572.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(38489..39247)
                     /gene="prdD"
                     /locus_tag="ACGI6B_001109"
     CDS             complement(38489..39247)
                     /gene="prdD"
                     /locus_tag="ACGI6B_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HFG9750573.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(39327..40052)
                     /gene="prdB"
                     /locus_tag="ACGI6B_001110"
     CDS             complement(39327..40052)
                     /gene="prdB"
                     /locus_tag="ACGI6B_001110"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(39600..39602),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HFG9750574.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(40090..40374)
                     /locus_tag="ACGI6B_001111"
     CDS             complement(40090..40374)
                     /locus_tag="ACGI6B_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HFG9750575.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(40455..42335)
                     /gene="prdA"
                     /locus_tag="ACGI6B_001112"
     CDS             complement(40455..42335)
                     /gene="prdA"
                     /locus_tag="ACGI6B_001112"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HFG9750576.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(42764..44524)
                     /gene="prdR"
                     /locus_tag="ACGI6B_001113"
     CDS             complement(42764..44524)
                     /gene="prdR"
                     /locus_tag="ACGI6B_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HFG9750577.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(44931..47105)
                     /locus_tag="ACGI6B_001114"
     CDS             complement(44931..47105)
                     /locus_tag="ACGI6B_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HFG9750578.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            complement(47260..47388)
                     /locus_tag="ACGI6B_001115"
     CDS             complement(47260..47388)
                     /locus_tag="ACGI6B_001115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750579.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     regulatory      complement(47687..47772)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(48177..49490)
                     /gene="prdC"
                     /locus_tag="ACGI6B_001116"
     CDS             complement(48177..49490)
                     /gene="prdC"
                     /locus_tag="ACGI6B_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HFG9750580.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(49879..50643)
                     /locus_tag="ACGI6B_001117"
     CDS             complement(49879..50643)
                     /locus_tag="ACGI6B_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9750581.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
BASE COUNT        16017 a         9090 c         6083 g        19495 t
ORIGIN      
        1 cctaaattaa tttattatat tttttaaatt aattttctat acttcttaaa tattgtctta
       61 tacctctgca atcttattat ggttactcat caaattattt tgctttatag tcaatgattt
      121 catttcatga actgcaaagt atatacatac cattgccgct gctgcatctg ctattggtcc
      181 tgcaaacatt actccatcaa ttcccataaa cattggtaaa attactatca atggaattaa
      241 aaataaaatt tgacgagtca aagaagtaaa aagtcccaac actgatttcc caattgatgt
      301 aaagaaagtt gatgatacta tttgaacacc atttataaat gttaagaaca agaaagttct
      361 aaaataatgt tctgcaaata taaaataagt ctcatttccg ttaccaaata tagacgtaat
      421 ttgtcttggg aagaattgga aacataagaa tgcaataaca gatataatag ttgcacttcc
      481 cactgcaaac ttatatgctt cttttacacg attatacttt tcagccccat aattataccc
      541 tataattggt tgcatacctt gtgatatacc aataattata gatataaaaa tcatatttac
      601 tttaataatg ataccgacac aagcaagtgg aatctcactt ccataaactg acctagcccc
      661 ataatatgcc aatgtattat tcataactat ctgtgttaca gtcattgcta attgattaaa
      721 acatgagctt gaacctagcg caaatatatg tttataaatt atactatcta atttaaaact
      781 tttcttatgt aatttaatat gttgaaaatt ctttatataa tgaacactta ttccaaaaga
      841 tataagttgc ccaataacag ttgctaaagc agacccagca attcccatat taaaaacaaa
      901 aatgaataat ggattcaaaa ttgtattaat aactgctcct attaacatac aaaacataga
      961 agcttttggt ctcccatctg caagtatcaa cttactcatt ccagtggaca ttataagaaa
     1021 tggtaatcca attgatgtaa tttttgtata tgtgtgtgca taaggtagta tttctgatgt
     1081 agagccaaag aattttaata gtggtgttaa aaatattgat actactataa ataaacttat
     1141 tccaaaaaca gccatcaagc atatagaatt tcctgctgct tttgtagctt tttctgaatt
     1201 tttagcacct aattccaatg aacacttaga cgctcctcca attcctaaca ataatgcaat
     1261 tgcagtacat attgttgtta gtggaaaagt gacatttgta gcagcattac ccaatacacc
     1321 aacaccttgc ccaataaata tttggtcaac aatgttatat aaagcactta ctaacattgc
     1381 tataatacta ggaattgcgt acttagttaa taactttgaa accttttcat attccaatgg
     1441 ctccttatat ttttcttcca ttttccttcg cctccaattt tgcttcttca ttcatatcaa
     1501 tagccaaaaa tgcaactttc tttaacattg acataaacaa ctcttgctct tctttctcaa
     1561 atgaactcaa caactcctca acatacccat ttgttatttt tagtatttca tcataacatc
     1621 ctaaagattt ttctgtagga tataaatgcc atgttctttt atcctttaca cctggttctc
     1681 ttgttataaa gccttctttt tctaaatttg ccaaagctct tgttatatta ctaggattca
     1741 caagaatttt ctcaaatata gtgtcctgtg taatacctgg attattacat atgtgtaata
     1801 cataaaaata ttggctatta ttgattccaa tatccgccaa tttcctgtca atataatttg
     1861 tattgtatct atttataatg gaaacccatt ttaatagctc tctcatattc tctcctttaa
     1921 tatctattgt ccttattttt ttgcgttcgc aataatctat ttgctcatgc aattatttta
     1981 ctgctaattt tatactttgt caatagcacg caagtttata tttaaaccat aaagttacta
     2041 taaattttat atattagact cttactttga tttaactatt tttaaatctt cattgatatt
     2101 taattacctt ttatattaat ttttttgcat atttatcaat aactcctaat tctttttagc
     2161 ttatcttaaa aagcatcttg acgtaagatt tattatctgt aataataaat atatcgaatc
     2221 gatatatatc taattgatac caggaggtaa tatgttagaa tacattattt taggattttt
     2281 aatggaaaaa gaattaagtg gatacgactt aaaacaaata atgtcagaaa gtacctcgta
     2341 cttttttgac gccagctttg gaagtatcta tcctgcactt aaaagattag aaacaaaagg
     2401 atatatacac tatcacgaag ttatagatgg tagcaaatta aaaaaattat attccataac
     2461 taatactggt aaagaagtat ttcttgaatg gttaaagaaa cctataaatt tttctaaaac
     2521 aaagcaagac caccttgtta atatattctt ttacaagtat ctacccaaag aaataattga
     2581 aacaaactta aaaatgttaa taactgaagt agaattactt ctaaataaat taagtaaaca
     2641 aaaaaatgat gcagaagaaa atcattgcgt tgaccaatat acatatatgt atgcaacaat
     2701 gatttatgga gttgactatt ataattttgt tattaattgg tgtaaagatt tactgaaaaa
     2761 tctataagat ataattttta gaagtaaaca tacataataa aaggagatgg actttatgag
     2821 gcttttaata catgatttaa ctgacaaaga attagaaaag ttattaccca aacttgatga
     2881 caatataaaa atacttccaa acacaaaaga aatccacaaa tgtattggtt gttttggttg
     2941 ctggattaaa actccttctg aatgtgttgt aaaagattat tacagcaaca tgggtaaact
     3001 tataaatgac tctgatggaa ttattataat aagcagatgc tgttacggag ggtttagccc
     3061 ttttgtcaaa aacgtacttg ataaaagcat taattatatt cttccatact ttgtaataaa
     3121 agataatatg atgcgtcata aaagcagata tgataaacag ataaatttaa aagctatatt
     3181 ctatggagat tgtattacag aagctgaaaa agaaactgct acgaaattag taaatggaaa
     3241 tgctataaat ttcaatataa aagaacactc tgtttgtttc tatgaaaata tacaagatat
     3301 aagaggtgaa tccatatgaa tatagtaatg ataaatggta gtccaaaagt aaataaaaat
     3361 aattcacagt attttttatc tgaattaaaa caacttattc aaaatgataa taaagttttt
     3421 acatttaaag tagatagcac atctaagtat cctgacgctc ttaatgccat acttaattgt
     3481 gatagattaa taatcgcatc tcctctttat gtagatggta ttccatctca tttacttcat
     3541 tttttagaga aatgtgaaaa atttttaaaa acacaaaata aaaaacctat tccagtgtat
     3601 gcaatagtta actgtggatt caatgaaggt gaacataatc atttggcaat tcaaatgata
     3661 gagcattggt gtaaaaaaat caactttaca tgggttcaag gtattggaat tggagctggg
     3721 gagatgtttg gttcactaga atccgtacct ttgggtaaag gacctaaaaa agatttagga
     3781 attgcattaa aagaactttc aaaaaatata cttagtgata atatacatat agatgattcc
     3841 aacaaaagta ctatttttat ttcacctaac ttccctagat tcctatataa acttatgggg
     3901 aatcatcaat ggaatactca agctaaatca aatggattga ctaaaagtga tatactaaag
     3961 aaataaaatt ttacttcaac aaatactaaa aaccgtcaag cacgaataaa ctattcatct
     4021 tgacggtttt ttatgttatt tatattcact agacatggga taattcacta taacattgat
     4081 tatctagtct aatatttatc ttattctttt tgtaaaatcc aagttgctcc aacaaggtcc
     4141 tgctgtaaat cagtatattc atttttttgg ctaataacat attgttcaaa catatctttt
     4201 tgagaaggat attttttaat tagctcattt gcacgagtta taatccaatt catttcctct
     4261 tcaacatcat tttcaagttc taaacatggt atacattcaa ttagcttcag acctaattct
     4321 ttgaacaata tacaccactc attgtaagtg ggatacaccc tctcaaaatt aagctgttca
     4381 tttgtagatt tatctactac atatgcatca tcaattaaaa tgaacccctt actttttatt
     4441 gtaggtaata gcttacttat agtttcttta taattaccta aaatatcccc tacagctcca
     4501 aaaatcacac aatcataatc atgctcaatt tttacagaac tattagcatc attcatttca
     4561 aaatcacaca aagcatcaac cttatattca atagcctttc ttctagcata attaataaat
     4621 tcaggtatca aatcaatacc tttgatatgt atattcaact cttttgcaag cttcacagat
     4681 acagcacctt ttccacaagc taaatccaaa acttttgtat cctcattaaa aggtatatgc
     4741 tttctaaata attgtaacat ttgttctggt gaacttccta attcccaaaa atcttgtaat
     4801 aaataaggca aataaggtaa aatatcagta gtatcagaag ttagtgactc tgccaatttt
     4861 tcttttacag tatcattcat tttttcaacc tccaaaattt aaattattat tcaaaagtaa
     4921 tattatttta tatacttctt cataggtact acaaacagtg gaaaatgctt ttgacctttg
     4981 tattttgcaa ttcatttctt ttctttatga gctgtaataa ttgtataact gtgagcgttt
     5041 actgttataa tacaatcata tacacttata taccagtttt ttccctttct attaataatg
     5101 gcatttggag attctatttt agttttacac cagttcacta catcatttgt ctccaaagaa
     5161 agatttttct taattcttac aacgcctaac tctgttgtat gcaatctatc taggttttta
     5221 agtaattcgt tttctacatt catttttatt aaaacacctt atcctttgca aaattaaatc
     5281 tattatttag tactatttta tcatagtatt gtgtatttta tcataatctt atagtaaaac
     5341 tatttctttc ctacagatta taaattattt atgaatacac accattatag aacttttcct
     5401 atgcaaaatt aaatctatta tttaacacta ttttgtcata gtattgtgta ttttatcata
     5461 atcttatagt aaaattattt ctttcctaca aactataaat tatttataaa tacatactgc
     5521 tgtaaaactt tgcctatata aatccatcta ctttatacat aataccttct tttaaatgat
     5581 tggaacccaa aactgaattt cactcaaacc attctcatca gttttttctc catattttac
     5641 aaagtcatat aaattatttt cattaaactg gcaacttgaa ttagggaacc attcttgata
     5701 gatatattct acaacatttt gcatactatc ctcatttttc ccaataaaag taaaaacaat
     5761 ataattactt ttcggtaata cgaatttttc cattccatta ggtaatttgc caaaagtagc
     5821 tacttgagca cctgcaatgt atgtaaatgt aggagtattc tcaatctctt taaaattgct
     5881 ataatcacta actccaatta aaaaacctaa atctacctta ttttctatca tctctttatt
     5941 tttatgcaaa tccctccagc attttccaat aatatgaaat ccttttctta ctttaccaga
     6001 atagccaaca atatttatat ctgcacaatt aactttttta acatctaaaa ccatatctcc
     6061 acgtaatttt tttctattat gaatatcaaa ttttaattgt agtggtaaaa aatcttgatg
     6121 tcttctaaaa aaattaggac tacgtttaaa cattttttta aaccctcttg aaaaagagcg
     6181 ttgtgtatca taccccgaga ttaaagcaat ctcaataatt ggtttttctg taaaaattag
     6241 cattctcgct gcttcagtaa gtctacgtct ttgtatataa ttatgtacac taaatccgac
     6301 aactgatgta aacattctat gtaaatggta ttttgaatag cctatctctt tagaaattac
     6361 atttaaatct aacatctcat ctaaaagatg tttttctata taatctaaag ttttttcaat
     6421 ggtaatcaaa ttgttatcca tatagcatcc ctcctatttt aaagatagca tattatttta
     6481 ttttgttttt cacattcttt gctaatattt tgatttattt tatggcacac aaaagacact
     6541 tctatttgaa gtgcctatct actctgtata ttgagcttct tatttcaatt tattataatc
     6601 ttcaacagat acaggttcta gccatttatt gaaagcacct tcagcagtta cctcgtctac
     6661 agccaactgc taaatgccct tctatacctt aatctaactt ttccacttca agtatacaac
     6721 ttgaagttag ctctaaatca atagttttta tcacaacaat ttgattttaa atttaaattt
     6781 attcttttaa attatcatca tctattatag attttcttat attcaataac tggtctacaa
     6841 attggttagc aggagtattt aaaatattct caggagtatc atactgttga acaactccat
     6901 gatttaatac caaagtcttt gttcccaaaa tcaaagcttc ataaatatca tgcgtaataa
     6961 agataatcgt aactcctgtt tttttatgaa tttcttttat ctctttttgt aattgctttc
     7021 gtgtaatctc atcaactgca ccaaaaggtt catccattaa taaaatttct ggagaagaag
     7081 ctaaagctct tgcaatccct acacgttgtt gctgaccacc tgacaattct gatgggtaac
     7141 gcactttcat atcatttggc aatcccacta tttccatcca cttgtttaca gcacttttta
     7201 cttctaattt attcttttta cttaggagat ttggtacata tgaaatattt tcttcaacag
     7261 tcatatgagg aaaaagaaca cttccctgta cacaatatcc tattcttctt cttaatttaa
     7321 ttaagtcctc gtttttgata tctgtttggt ttatcaaaat atttcctcca tctggcaaaa
     7381 tcagaccatt aatcattttt aagagtgttg tcttaccaga accagacgaa ccaattacag
     7441 tcaaaaactc tcctttttct atatctaaat taagattatc tattatagtt ttatcattat
     7501 attgtttttt aatattttta aattgtataa taggtgtcat tatatttcct ccaatttata
     7561 aaagaccttt cttattcaga aactcacgag caacttctct aggttctaaa ttgttgtttt
     7621 ctacgtcata attcatttta gccatttctt tatcagtcaa aatatctcca accatcttaa
     7681 atacctttga tagttctgga ttttcctcta atacttccaa acgaacaaca tttccacatt
     7741 gataagatgg gtagaaattt ttatcatctt ttagaactac aacatctgaa acactatact
     7801 gaccatcagt tgtaaatata ggcattacgt caatcttacc ttcattgata gcttggtatt
     7861 ttaatccaat atctaaatca actgtatctt taaagttcag tccatatgta ttacacaatg
     7921 gagtatatcc atcctctctc tcataaaagt catattcagc tccaaatgtc aatttagaag
     7981 atacatcttt taagtccgtt atatttttta aattgtattt atctgcaact tcttttctaa
     8041 ctgctaatgc atagctatta gaaaaaccat acataccagt ccattgcatt ttaagtttag
     8101 ttttgtattc cgactgtagt ttactaaaca tactttcagt atataattca tttttcttaa
     8161 gtaccatatt ccagcctgta cctgtatact ctggataaat gtcaaattct ccattctcca
     8221 tagctggctg gatattagat gttccccctc ctacaccttg tgtaatttcc acgtttagac
     8281 ctgtgttttt ttcaattaaa atatccaaca tctcaccaag aatatattgt tcagtcatag
     8341 gtttagtagc aatatgaata gtttgagtct ttgaaggatt aaataaataa gacgtaaaga
     8401 taattactat aagaataagg cttcctagaa tggcaaaaac ttttttccta tttttctttt
     8461 ttctttttcg ttgaaaaaat ttttctacca gccctaaaat aaaatcaaat attaaagcta
     8521 atagagcaat aagtatactt ccagtaattg tcattattgt gttatttgtc gtgattcctc
     8581 tatatatggc tacacctagg cctccagcac ctataaatga agcgatacca gctaatgcaa
     8641 ttgtcataat caccatattt ctaataccag acataataac aggtagggct aacggaagtt
     8701 ttattttata caatatctga aaatctgtac ttcccattcc cctagctgct tcaagaatat
     8761 ttttatccac attagtaatt ccagtatatg tatttctaac cattggcaat aatgcataaa
     8821 tggtaagtgc aataacagca gtagtatttc caattccgga aaatggtatc agaaatccta
     8881 gcatagaaat agatggaatg gtgtatagaa aattgatagt tcccaatata ggtttagaag
     8941 aattatgaaa ttcacttaca ataataccaa ttataccacc caaaacaatc gcaattatta
     9001 tagaaataat agaaatcatc aaatgttcta tggatagctt tataaaaaaa tcacttcttt
     9061 cagataataa ggttagaata tcatttatca tccatctctc tccttttata agttttactt
     9121 aacaagattt aaattttaat gtagttaatc tttattgttt atacatcagc aaatcgtcat
     9181 caaattattt cacaaattat atattaagat tataatctga acctcccatg actgaagtca
     9241 caagtgtgta tagccatttt ttaatcaaac ttgcaagtag acacaaaaaa gtaactatta
     9301 gagactccat cctcgatatt ctccataaaa ataagaaaaa aggtcagaac cgttaaaata
     9361 ctaaaattta aacaaatttc tgacacttta tttaattaaa tttgaatatt tctcacatta
     9421 tttattactg ttaagtttaa ttatcatttt atatacccat tatatttatt aaaatatcat
     9481 tataatttta ttatatttta tttattatac tatatatttt gctgttaatg atattttatt
     9541 gttacataat tgttttggaa taccttcaaa tattactctc ccgccttttg ttcctccttc
     9601 aggtcccagc tcaataatcc aatcagctct tttaattaca tctatattgt gttcaattat
     9661 tatgaccgta tttccattat ctacaatctc ttcaattata cttataaaat tctcaatatc
     9721 agccatatga agacctgttg ttggttcatc taatatatac actgaacttt ccttatttaa
     9781 ttcactagca agctttaatc tttgacactc tcctccagac aaagtatcaa gagtttgtcc
     9841 taaagtaagg tatccaatac ccattttttc aatacattgt aatttcgttt taatctgttt
     9901 taaattaaaa aactcaattg cctctaaaac agacatttca agaacttcaa ttatattctt
     9961 cccttgaaac ctatattcta aaacttcttt tttaaatttt ttaccttcac aaatattgca
    10021 ggtactcttt atactctcca taaaagcaag attcatttct actattccag aacccttaca
    10081 gttatcacaa gctccttctg aatttgaact gaataaagaa gcatttacgc catttgtttt
    10141 tgaaaatatg cttcttatgt tgttcataat tccactatat gttgccaaac tcgaccttga
    10201 atttgctgaa acagggcttt ggtctataag tacagcacta ggattttgct ttaaaaattc
    10261 atccttaatc aatgtacttt tacctgctcc tgctactcca gtaactaaag tcaatacacc
    10321 tttaggtatc ttaatagata cattttttaa attgttttta ttacaatttt taacttctaa
    10381 atatccacta tgttctctta cactttcttt aataggaaga aatttactta aagcattccc
    10441 tgtcaaagta ccacttgtaa gtagattttc ataactgcct tcatacacaa cttctccacc
    10501 atacttacct gcctttggtc ctacatctac aatatggtct gcaatcttaa taacatctgg
    10561 gtcatgttca acaactataa ttgtattccc tttatcacgc aattttttta aaagattatt
    10621 taatttatac acatcttttg gatgaagtcc tacacttggt tcatcaaaaa tatacattaa
    10681 atcaacaaga ttactattta aatacttaac catctttata cgctgagatt cccctcctga
    10741 tagagaagat gtctctctat ctagtgttaa atatccaaga cctatatcta tgatactgtt
    10801 taacttttct attattcctt taattaaagg ttgtgcttct ggctcatcta tacttttaat
    10861 aacttcagcc aattcatcaa tttgcatact agttaaatct gctatattat aaccattaat
    10921 taaagaccta tatactcttt cattaagtct tcttccatca catataggac atctatcttc
    10981 tataagaagt ttactcaatt ttttcttagt agcttcagat atttctccct ctctacttaa
    11041 gtataggcga ttaaatttaa ccattaatcc ctcatatgtc atgttagttt gaccagcatt
    11101 ttctatctta atcttttctg ctttaccatg caaaaacttt tccaattcta cttctgaata
    11161 atcacatatc ctcttattaa tatcaaaaaa tcctgattca gcgaatatat tccaatgcca
    11221 agaacccact ccaaaccctg aaagcaatat agcaccttca tttaatgatt tattcatatc
    11281 aagaatttgg ttcatattag gaactagctt ttttccaagc ccatgacagt gtgggcacat
    11341 accatcaata ttattaaaag aaaacatatt ggcattacct atatactcac taccaaacct
    11401 tgaatataaa gctcttaaaa aggaatttac atctgtaata gtacctagag ttgaccttga
    11461 atttccacca agtctacttt ggtctatgat aattggtgtt gacaaatttt ctatacaatc
    11521 agctttaacc tctccatact taggtaaaaa acttcttaca aatgtactaa acctttcatt
    11581 tagttggcgt tgtgattctt ttgcaattgt ctcaaataca atagatgact ttccagaacc
    11641 cgacacccct gtgaatatgg ttatttttct ttttggtatt tttaaggaaa tattgttcaa
    11701 attgttctct ttgcaacctt ttaaaattat aaattcattg ttcatatttt acctcctgca
    11761 ttatttttct ttaaattatt tagagaaaat cgtaataata gttgttagtt atacataagt
    11821 gaaatactat tttattagat tactcctttt tttataaaca caggtcaaat tatagcatgt
    11881 agattttatc tggtagtata gtgtagggtt tggattttta taaaaaatac agttatttta
    11941 tttaagcgta attacatcta ttctacctaa atatcatcat tatatatgag tgttaaatta
    12001 tataaaattc ttaagttaat aatctcaaaa tggtattagt tacataaaaa aagtatcttg
    12061 cctatgattt taaaaccata aataagatac tatgttttac actgtttctt ctttcacaaa
    12121 taattctata caattattca acttaacttt aaaattttaa acactaaaaa cctctatctt
    12181 taaatcttta aataaaacta aatttccaat accttcaaaa ctatagaaaa ttccaatcaa
    12241 cttatcaaac ataacttact acttataaga aattaatctt cacttaatac agacgtaagt
    12301 tcagggtcat ttagaagatg ttcaaaataa ctatgtgccg aaatatatcc tataccatac
    12361 attttacgct ttgaatttac aaccttatat tcataacaag cactcttaac cttattgtag
    12421 tcatcaaaat ccattgcttt atagatagct ctttcatttc ttggtatata ctttacatat
    12481 tttggcttaa caaaatacaa gtcatctttt atcttacctt cacgtttaag tttctttaaa
    12541 acttccccat tctttctatg cattatatgg tcatcatact tatagtgatg ggctatatgg
    12601 gtcacattgc ctttaaactt atgttcaaaa tccaatatag tcttttccat taattcataa
    12661 tgtgtgcctt cctttttatc tatatctgca agtataatca cattttgagg tttcactcca
    12721 agctctctca aagctgattt aaactcgtta tttctaagtt cttccttttg cttcctagtt
    12781 aaatttctaa attttgtgtt agctttgaaa acgttaactc cagaaccatc tgatactaat
    12841 accacatata cattatcaac accacactgt tttatagcat ctacaatggc acttcctccc
    12901 cataaaacct catcatcttg atgttgagga taaaacactg ctgctgattt aaattttcta
    12961 ctattactct caggaatagg tatttcaagt tttccgttaa aattctttgc aattaaaaat
    13021 atcatcaata atattaatat acttattcct atgtacttcg ttttactttc catatttaaa
    13081 attttctcct tcttctgaac ctcccatgat taaagtcaca gggttactac ttcatagaat
    13141 ttcgtataat caactcaaat atcttataaa cctttgtgtc aatcaggtct aaatatacaa
    13201 attattttac aatattttat tgtttcatac aatctttgac acgaactata ataaaataaa
    13261 aaagccttct aactgcaaca ttgcaaacat tacagccaga aagcttctta attcttaaaa
    13321 tttattaagc ttaaaaattt attaaactta ttttttaata taaatatgtc tattattaat
    13381 aaattatact ttaattacta tatcaatacg actttactat attactataa cattacttat
    13441 atcaatacaa ctatttttat agtctacatt tcctttaaaa tttcaagcaa ctcttcttca
    13501 ctatacatat acttcttatt acagaactgg caaccaactt ctgctttttt atcttcttct
    13561 attatttgag ccaatgattt tttaccaatt gcaaccaaag cagtttttac tctttctttt
    13621 gaacattcac acataaaccc aacttcacag gtatcaagta ttttaggttc caaaccatcc
    13681 aaaacaatat ttaatatatc ctctggtgat tttccttctt ttaacatgtt agttatagaa
    13741 ggtaatttag ctacattttc ctctaactta gcaatagtct cttcttctgc atctggcatt
    13801 aattgaacta taaatcctcc agcaaactct acttcatctt cttttgtaag aacacctaaa
    13861 gctactactg atggagtctg ctctgataca gcaaaataat aagtaaagtc ttctgcaatt
    13921 tctccactta ccaatggata agtcccatta tatggttctc taagacctaa gtcttttata
    13981 acttttacaa caccttctgt acctactgct gctgctacat ttaactttcc atttggatag
    14041 tcttcaacct ctacttgtgg atttcctaca tatcctttta ccattccctt agagtctgat
    14101 gtggcgataa tagttccaat tggacctcca ccttttatta ttacagtcaa tttatcacta
    14161 tcatttttca tcattaatcc cattatagat gtggctgtta atgttcttcc aagtgctgct
    14221 gttgctactt ttgtagtttc atgtaatctt ctagcttctt ctactgtatt tctagtagtt
    14281 gcgacaaatg ctctaacttg accatttcct gatgtcgctc ttagtacata atctctcatt
    14341 aatattcctc catgtcccgt taattgtctt atttttatta tattactacc ttaattttaa
    14401 tctataaata cttatctatc ttaaccttgg gcaaaaccca ttttatttta attttactaa
    14461 gcaatagtta ttatatttta atttttatct ataaaacttt ataattatta gaaaataaaa
    14521 atcataaaat atcatcactt tacttttaat ctataaaact gcattttata tatttttata
    14581 tcatggttat ttttaataat tatcttatat agtcaagaat atcttatttt ttacatacaa
    14641 agaagattct ttgactttct tcactaggtt tgtcaaatgt aaaatcccca taatatttta
    14701 tatccttaaa tccagatttt tcaagcatat caactatctc atcttcagta tatgctcttt
    14761 gttggtgtat ctcttcaaat ctttcaaact ttccacattc atctttaata aagaatgtaa
    14821 gttccatttc aattagattc tcttcttcat caaagtaatt ttgccacata tacgctatat
    14881 cttctctatt ttctccatac atgttatttc ccaatatgtt ggctaattta taaaaagaac
    14941 ttatatcaaa tatgaatatt ccatctttct ttaataattc atatgtcttt gaaaatacat
    15001 tttccaaatc atcatcataa gttatataat taaatccatc acaagcacaa agtacacagt
    15061 ccaaatcaga tatttcaaaa tcaagctctg atatatcttg ttgaagtaaa actaattcta
    15121 ctccttcttt ttctgccttt tctcttgcta cactaagcat ctcatcagat atatctatac
    15181 cagctatatc gtaatttttt ttagtcaatg gaattgtaag attacccgtg ccacatgcca
    15241 attccaatat attttttact tttacacctt cactatctat tatatcttct atatatttaa
    15301 cccaaccatt ataatcaact tcattcataa gttcatcata tacaaacgca aaatcactat
    15361 attgactcat cctttttatt ctgctcctcc acaatctttt taagcctctt tttgcgtgat
    15421 gtaagcatac ctccaattct tcctgcttcc ttagctgtaa gtcctgccca tccacgttca
    15481 ccaactttat ccataagacc tagttctgag gctatctcat atttcattat atcatcattt
    15541 gtcaatattt tagtattctt gccattagtt atcttctttt ctctttgtac tttttctttc
    15601 aaaaaaatca ccccttctta tttgtatttt tgtcaattaa aataaattta tacaaatatg
    15661 taaggggtat ttattaatac acttttttta aatttaggta tttatgcttt ttaatcttta
    15721 taacttatct tcctaatcta ttgcttcttt gtattttaat aattttgaat aaacattcaa
    15781 tgcatttaca catatctttt catcaaaatt aaagtttata ttatgaagac catttacaaa
    15841 tcctttttct tcgtttcttg aacccaacat aaaaaataat cctggaactt ctttttgata
    15901 ataagaaaaa tcttctgata tcataagtgg ctctaactct acaacagtat cacttccaat
    15961 agcctctaca aactcttgat acaaatcctt gtcattttta acagcaatat aatcatctat
    16021 aatatttata ttaaccttac agttataagc caattcaaac cctctagcaa gttctctaac
    16081 tctaagcttc attttctcat acacttctgg gctgaaacat ctcatagtac cttccatctt
    16141 tatattttca gctattatat ttcttctagc tcccccttca attctaccaa tagtcaacac
    16201 tgcattgtct attgggctta tatttctaga cactatagtt tgaagtgagc ttacaaagtt
    16261 tgaagctatc actataccat caataccatt ttgtggcata gccccatgtc cacttttaga
    16321 cactatttct atgtcaatat ctcctatttg agccataaaa tagccctctc ttgtacctac
    16381 ataaccttct ggtaactctg gatatatatg aagcccatat atttcatcaa tattgtattt
    16441 tctaagcact ccttcatcta ttaaagcttt tgctccccca ggaccttctt ctgctggttg
    16501 gaatataaat actatattat catttaagtc ttctttatta tcatttaaat actcaattaa
    16561 tccaagtaat atagtagcat gtccatcatg accacataaa tgttttaccc cttcttcact
    16621 atgaagacca tctatgtcag ctctaaaagc aatatctttt ttaggtcttt taccttctat
    16681 tacacctact atacctgttt ctaaataaac ttcatactca attcctaatt tatcaagata
    16741 atctcttata tattttgaag tcttatactc ttttctccca atctctgcaa taggatttaa
    16801 tgacttccta tgtttataaa gttgttcttg taaatatgat gcctctctca tgtaatctcc
    16861 tccaatttct aatttattaa aaaatatttc tatatttctt tataattaaa atattatata
    16921 gcatgttata ctaattaaaa ataaaaagct aaattgaata taaaaatatt ttgcattatt
    16981 aaataattca ccattaataa taattcccct gtttcataaa tcaaaacaga ggaattatta
    17041 ttggaaaatt ttatttttag caagagaaga aatgtttagc aagtccaccc ttagctgttt
    17101 ctttgtactt atccatcata tctataccag tttctttcat tgtttcaaca acttggtcta
    17161 aggagacaga atgtgagcca tcagttaata atgcataatt agctgcatca taagctcttt
    17221 gagctgccat tgcatttctt tctatacatg gaatttgaac atatccatat acagggtcac
    17281 aagtcattcc taaatgatgt tcaagtgcta tttctgccgc atattcaata tggtctatag
    17341 ttccaccttt taagtatgct actgctgctg ctgccataga acaagcggct ccaacttcac
    17401 cttgacaccc tacctctgct ccagatattg aagcgttagt ttttattata ttacctatta
    17461 ttccagctac aagtagtgct tcaattattt tttcgtcatc ataattatag aaatcctgca
    17521 ttgcaaagaa tattcctggt attactccag cagaaccaca agttggtgct gtaactatta
    17581 tattaccaga agcgttttgc tctgaagctg ctagtgcata tgcatataca agtgttgaga
    17641 agctcttatc ttttttatat gcattataaa atgtacttgc tcttcttttt aataataact
    17701 tgcctggtat tatttcatct gtacttagtc catcatcaat agattttctc atagcatctt
    17761 ttatagtttt tatataatct tttatgtctt ttggttcaca ttccaacaca aaatctacca
    17821 aagtcttatt attttctttg caccatttta ctatctcatc catagtatct aagtgatata
    17881 tactattgct tttactgttt ctttgacctt cttctgctat agtccctcca cctacagaat
    17941 atacagtcca ttcagctgtt acatttttat ctttatctaa agcttcaaac ttcatcccat
    18001 ttgggtgaaa atcttttata atatcttctt cccaaacaat ttcgactttt ttaggtgcta
    18061 tagttttttc aattatatag tcagttaagt gacctttccc tgtagctgcc aaactaccat
    18121 ataatattgc tctaaaactt tctgcatctg ggttttcatt cttaaatctc tctgctgctc
    18181 tttgtggtcc catagtatga gaacttgaag gtccacttcc tattttaaaa agttccttta
    18241 aagtatccat attattcctc ctaaatttac ggtattattg cttattgtta gattttacgc
    18301 acatatttat tataacctaa atatattaat tattagttat taaattctca taaaatttat
    18361 tttttatttc cattttggta tattttatta aattaatatg catattttga gaataaaaca
    18421 tttatttaaa atttttctaa tattaatata atataaatat cttatttaaa cagttaagat
    18481 attaactata cctattaaaa ttaattaatt tgtactataa ttattattgt acttaaaaaa
    18541 ttaaatttag ataaaaaagg agatttgatt atatgttatt taaaggttct gctgttgctt
    18601 tagttacccc atttacagag gataataatg tcaactttga aaaattaggt gaattaatcg
    18661 aatatcacat tgagaatggc acagatgcac tagttgtttg tggaacaaca ggtgaggcga
    18721 ctactatgag tgaatctgaa atttttgctg taataaaata tacagttgaa aaagttaata
    18781 agagaatacc tgttatagca ggtactggtt caaacaatac tatgttgtct gttcatatga
    18841 gtcaagaagc tgaaaaatta ggggtggatg gtcttttaat aataactcct tactataata
    18901 agactaatga gaaaggtctt aaacttcact ttgaaacaat agcaaatagc gttaagttgc
    18961 caattatttt atacaacgta ccaggaagaa ctaaagtgaa tataaagcca tctgttgtgg
    19021 ctgaacttgc taaaatagat aatatagttg ctgtgaagga agcaagtgga gacttagctc
    19081 aagttgcaga aatagctaaa ttagtaccta aagactttgc tatatactcc ggtaatgatg
    19141 atactatact accactatta tcattaggtg gaagtggtgt tatctctgta cttgctaaca
    19201 tatgtcctaa agaaacacat gatttagtaa ctaaattctt tgaaggtgat attgaaggct
    19261 caaaaaaatt acaacttgat atggatgctt taattgctgc tttatttata gaagtaaatc
    19321 cagttcctgt taaaactgcc atgaacattt taggttttaa tgttggtgat ttaagacttc
    19381 ctctagctga aatggaagaa actaatctta atgtgttaaa acaagaattg acaaattttg
    19441 gatttaaatt ttagcaatat ttatataaat aagttgattt tttcttaaat atgtattatt
    19501 attagaataa tcaaatttta atatcctatt aacctagggt agaggtagct gtaactaaga
    19561 gtatccaata gtagctggca ggtgttaatg attggaaaaa ggatttacag ccgaagaaaa
    19621 tatttcttgc aataatattt tctgggattg cacataacat gtgtaatact gtcacttatt
    19681 tttgtaggtg atgagctaca gggtttactt tgagtgataa attagacgta caaagtatac
    19741 ctttgtacgt tttttgtata taattaaagt tgttaatata atattaaagt ttttggatat
    19801 ataaaaatat taacatatct tacttgtata actaaaataa aataatgttt taataattta
    19861 aaattggggt gaatttatat gaaaaaagta aatgtagcaa tagttggtgc tactggaatg
    19921 gtaggtagaa catttttaaa agtattagaa gaaagaaatt ttccaataga aaatctattt
    19981 ttattctctt cttctaaatc agctggttct aaagtaatgt tttgtggtaa agaatatata
    20041 gtagaagaat taaaggaaac ttcatttact gatagaggca tacaaatagc attattctct
    20101 gctggtggaa gtattagtga aaagtatgct ccaatagctg cttcaaatgg aatcttagta
    20161 gtagataact ctagtcaatg gagaatgaat ccagatgtgc ctttagtagt tcctgaggtt
    20221 aatccagaag ccataaaaaa tcataagggt ataatagcta atcctaactg ctctactata
    20281 caagctatgg ttccccttaa agctcttgat gataaataca aaattaagag agttgtttac
    20341 tctacttacc aagctgtatc tggttctggt gttaaaggtg ttgaagattt agagaaagga
    20401 atgaacggta atcctactgg attctaccct catcaaatct cttacaactg tcttcctcat
    20461 atagattcat tcactgaaaa tggatacact aaagaagaaa tgaaaatggt aaatgaaact
    20521 atgaaaatac ttgataacta cgatttaaaa ataactgcaa ctactgtaag agttccagta
    20581 aaaaatggtc atagtgaatc tataaatgtt gagtttaaca atccatttga gttagatgat
    20641 ttagtaaaaa cacttgaaga agctccaggt gttgtagtag ttgataatcc tgctaaaaac
    20701 gaatatccaa ctgctgtaga atttgatgat agagatgaag tgtttgttgg aagaataaga
    20761 agagatttta gtgttgataa tggtgtaaat ctttgggttg ttgctgataa tataagaaaa
    20821 ggtgctgcaa ctaacgcaat tcaaatagct gaaatggctt tatcatacga tttagtataa
    20881 tatacaaact tgtataaata tataattttg taaaatatta atagtaactc aaaaacaagc
    20941 attagaagta actgtaatat aaaacacaaa aataaaaaag aggggattga ttttatatga
    21001 tatttaaagg ttctgctgtt gctctagtta caccatttac aaaggataac aaagttgatt
    21061 ttgacaaatt aggtgaatta gttgaatacc aaatagctaa tggtactgat gctatagtat
    21121 cttgtggtac tactggtgaa gctaatacta tgactgatga agaacaactt gctactatta
    21181 agtatgttgt tgaaaaagtt aataaaagag ttcctgttat agctggaagt ggttctaatg
    21241 atactatgca ctcagttaac ttaagtcaag aagctgaaaa attaggagta gatgctctat
    21301 taataataac accttattac aataaagcta ataaagctgg acttaagcgt cattttgaaa
    21361 caatagctaa cagtgttaaa cttccaatta tactttacaa tgtccctgga agaacttgtg
    21421 taaatataag tcctagcctt atagttgaac ttgctaaaat tgataacata gttgctgtaa
    21481 aagaagctag tggagaccta ggacaggttg cagaaatagc tagtttagtt ccagatgact
    21541 ttgctatcta ctctggtaat gatgatacta tacttccttt attatcttta ggaggtcaag
    21601 gagttatatc agtacttgct aatgtttgcc ctcaagaaac tcacgatttg gtagctaaat
    21661 tctttgaagg cgatattgaa ggttctagaa aattacaact tggtatggat gctctaatcg
    21721 cagctttatt tatagaagta aatcctatac cagttaagac tgcaatgaat ttattgggat
    21781 tcaatgttgg agatttaaga ttacctctag cagaaatgga cccagcaaat ttagaagtat
    21841 taaagaaaga attagttaac tttgggctaa aagttaatgc atagtaaaga ttaatgcatg
    21901 gtaaaaagta agtttggtca aaagttagcc cttaatcata ggaggaaatt atgttaaaag
    21961 ttatagcaac tggttatgat ggaaaaatgg gtaagatttt agccgatact ataagagaag
    22021 ataatgaatt agaattggtt tgtgttgcag ctagaggtct ggatagctat gaaggcgacc
    22081 ttaaaattta tgaagatatg agtactatag ttgaggaagc tgatgtagtt atagattttt
    22141 ctcatcactc taatttagat aatatcttat cttacgtttt aaagactaaa acacctttag
    22201 taattgctac tactggatat aatgatgacg aaatgaacaa aatacgtgaa gcggctaaag
    22261 ttattcctgt attccaatca tataatatgt ctcttggtgt aaatgtatta gtaaaattgg
    22321 ttaaagatgc agctaaatta ctagaaggat ttgatataga aataatagaa aaacatcaca
    22381 acagaaaagt agatgcccca agtggcacag ctgttatgat tgctaacgct ataaaagagg
    22441 ttttgccaaa cgttgaaaat aattatggta gatatggtcg tagtgcaaaa agacaaccaa
    22501 atgaagttgg tattcatgct ataagaggtg gaaatattgt tggcgaacat gatgctctat
    22561 ttgctggaga caatgaggtt cttacaataa gccatcaagc tcaatctcgt ggtatctttg
    22621 caagtggttc tatagctgct gccaaatact tagttaagca aaaaccagga ttttataata
    22681 tggacagcat gttgggataa tagcataaat ataataatac acaatataaa ttaaacatat
    22741 gcaatataga tataataata cgcaatacaa atcaaacata tataataaat ttatttaata
    22801 ctatgtctat acaataaaaa ctaactaaaa taaaatataa ataataagaa gtaagtatat
    22861 actaaaattg acaaataagt atataataaa agaaatataa tgaaatacaa ggagaattaa
    22921 caaatggtaa atttaaacga tgcttatgaa atagcaaggt atataaaaga agttaaaaag
    22981 aaaacacctg ttaaggtata tgtaaatact aactctttac acttccaatc aactgataaa
    23041 tttaaggtat tcggaagtaa tggttcttat atatttgtag gtgactatga ggatataatg
    23101 gaagtattag ataaacatgg aaatgatata acagatactc atgtagaata tgatagaaga
    23161 aattctgcta ttcctctaaa aaatacttta agtgaacatg caagaattga gccaggtgca
    23221 ataataagag acatggttac tatagaaaaa aatgctgttg taatgatggg agctgttata
    23281 aatataggag ctgttattgg tgaaggttct atggtagata tgaatgctgt tattggagct
    23341 agaggaactc ttggtaaaaa tgttcactta ggtgctggtg ctgttgttgc tggtgtttta
    23401 gagccacctt ctgctactcc tgttatagtt gaagatgatg ttttaatagg agctaatgca
    23461 gttattcttg aaggtgttag aatcggtaaa ggtgctgttg ttgcagctgg tgctgttgta
    23521 actactgatg tagaagctgg tgccgttgtt gctggttctc ctgctaaagt tataaaaatg
    23581 aaagatgaaa aaacggctga caaaactaag cttatggaag accttagaaa tttagattaa
    23641 ttaaatagta ctatactatt aattaaacaa taatatacta atttaaaaaa ttaattaaaa
    23701 aggtgtctct gtgtgaattt aattcaccag aggcaccctc ttttttacaa tatttttatc
    23761 ttttatttat aatcatttta tttttttaca attctcttat tcattcttat atcattttta
    23821 tttcttgctg acaagtattt ttatttcctt caatttttca atatatcaat gtaataaact
    23881 attaaacttt ttataattta ataaagtgaa aaccattaat ctttatataa tttaataaaa
    23941 tgaactacta aattttctat atatcaatag aataaattat taaacttttt ataattcaat
    24001 aaagcaaacc attaatcttt ctataattta ataaaatgaa ctactaaatt ttctatatat
    24061 caataaaata aactattaaa ctttttataa tttaataaat agaactgctg tttaccactg
    24121 gttgtgcatc tttattgcca caaagcacaa ctaacagatt acttaccata gctgctttac
    24181 gttcttcgtc caaagtcaca acattatttt cacttaaatt agtcaatgcc atctctacca
    24241 tacttacagc cccctcaaca atctttttac gtgctgctat tatagcctct gcttgttgac
    24301 gttgtaacat tgctgctgca atctctggtg cataagaaag atgagtaatt ctcacttcac
    24361 acacttcaat tcctgcaata tcaacccttg attgtaattc atcttttaat ctatctgcta
    24421 tttcctgact actaccacga agagattttt catctccatc ttcagataca tcatatggat
    24481 aaagtcttga aacattgcga atagtagaat cacactgaat agataagaat gtattataat
    24541 tatctacatt aaatactgcc tttgttgcat ctataacctt ccaaattaca accactccta
    24601 ttataattgg atttcccaat aaatcattga ctttctgctt ttcattatta agagtcattg
    24661 tttttaaaga tacttttttg ccccttgaat tagttgatat atctaccttt tcatcagatg
    24721 aagatttcga tacattaatt cttgatactg ctgggtttat tgctgaacaa aatggattca
    24781 cccaatagta cccttctttt tttatagttc cataatagtt accaaacaaa actagaacta
    24841 tagcttcttt aggattaatc atttttagcc caaaaaatag gataaaatct actactataa
    24901 atacaagtcc caaaattaac ataagtgaac ctattccagt aaatccacta tcaagtttta
    24961 ctatcccaaa aaccatgata cctattgaaa ctactaaaaa taataaaatc attatcaaag
    25021 ctgccactcc attgaatact tttgcgtctt tctcttgata agttttttct tcttttaaca
    25081 tatctatcac tcccatctca gtatattaag ttaagatatt tctaatttaa taataacact
    25141 tttaacaatt aaattgttga atttaaaatt aatactatat gtttatatat tttttataca
    25201 tctttatgca tacatgtatt tagttatttt aatgtattaa tattgatttc caaataaatc
    25261 atatgatata tgaatatggt atttcaaaat taacttttta gttcatgggg cactcttttt
    25321 ggtatgaagt caaatatatt tttatcattt atacaatgaa aaagaaattt atctatattt
    25381 tgaaaaatcc acttaaattt tttaatgtaa gataaattgt aagcttatta tcactaatat
    25441 tattttatat aaattatatt agtgataaaa taaacttata gaaatataac aaatttttat
    25501 taatttcata ggaggaattt atgttgaaag ttataattag tggctataat ggaacaatgg
    25561 gaagagtttt agcagaatgt gtaaaaaaag ataatgaatt agagttggtt tgtggtgtag
    25621 ctaggtctgc tgatgaaaaa ggtcatcata gtgatacaaa gctttacaaa acaatgactg
    25681 atgtaactga aaaaactgat gtaataatag acttttcaca tcatgagact ttagagccaa
    25741 tattgtcata tgctctaaaa aataatacac ctgtagtatt agctactact ggatatgatg
    25801 aaaatgactt aaataaaata gaagaagcat caaaaaacat tcctatatta ttatcatcaa
    25861 atacatctct tggagtgaat atattaataa aattagttaa ggatgcttca aagatgttag
    25921 aaggttttga tattgaaatc atagaaaaac atcacaatag aaaagaagac gcccctagtg
    25981 gaactgctac tatgatagct aatgcaatta gagaagtctt gcctgaaagt gaaaatacac
    26041 atggtagata tggtagagat tgtaaaagaa aaccaaatga aattggaata cactctataa
    26101 gaggtggcaa tataatcagc gaccatgatg ttgtatttgc aggtgataat gaagttctta
    26161 cattaagcca tcattctcaa tctgatgaag tatttgcaaa cggttctata gttgcagcta
    26221 aatttttaat tggtaagcaa tctgggcttt ataaaatgtc agatatatta ggataagttt
    26281 tgtacataat aaatctcaat ctatttaggt attcatattc atcaatttga atatctaatt
    26341 gattgagatt tctattctaa aagtttataa attttatgtt taccttttaa atataatcat
    26401 ttttcttaaa gattatctta tttattggta tatggaatta tattgtttat tattttataa
    26461 tctcttcatc atataaacct attaaatatt atcctattta ttggtatatg gaatcatatt
    26521 gtttattatt ttataatctt ttcatcatat aaacctatta aatattatcc taatttattg
    26581 gtatatgaaa tcatattgtt tattatttta tagtcttttc atcatataaa cctattaaat
    26641 attatcctat ttattggtat atgaaatcat attgtttatt attttatagt cttttcatca
    26701 tataaaccta ttaaatatta ttctatttat tggtatatga aatcatattg tttattattt
    26761 tataatcttt tcatcatata aactattgta ttctataaac tgatacatat ccagctgcag
    26821 aagtacctgt cgtgcctgaa ccaaatgtaa aacttaaagt aacagctgct gctctagcat
    26881 caactaaaaa gcttaaactt acacttacat ctgatgaagt aagtgtattt actgcttttg
    26941 caataaagtt attttgagct actccattaa agttagtagt aattgacata aatcctgcaa
    27001 gtgaaagtgt ggcacgtata ccgaaagata cattgtatat ccctccaact gcaagattta
    27061 tagtactact tgttggaaaa gttatattag ttccatttat aaatgatgaa ttaaatgtca
    27121 ctaatgtatt attcactaaa cttgaagagg acactgtaaa ttgtgcattg tttgctgcta
    27181 acactcctgt tgctccggtc gctccggttg gacctgttgc tccatttgct cctgttgctc
    27241 cggtcgcccc tgttgctcct gtcgctccat ttgctccagt tgggcctgtt ggtcctatcg
    27301 ctcctgctac tccatttgct cccgttgctc ctactgctcc ggttgggcct gttgctccat
    27361 ttgcccctgt tgctcctgtc gctcctgttg ctcctgctac tccatttgct ccggttggac
    27421 ctgttggacc tattggacct gctactccat tcgctccggt tgctcctact gctcccgttg
    27481 ggcctgttgc tccatttgcc cctgttgctc ctgtcgctcc ggttggacct actgctccat
    27541 ctgctcccgt tgggcctgtt gctcctactg ctcctgttgg acctattgcc ccagctactc
    27601 ctgttgctcc tgtcgctcct gttggaccta ccaaaccatt tgctccagtt gctccggttg
    27661 ggcctgttgg gcctaccaaa ccatttgctc cagttgctcc ggttgggcct gttgggcctg
    27721 ttggacctac caaaccatct ggacctgttg ctcctactgc tcctgttgga cctacctctc
    27781 catctgctcc tgttgctccg gttgctccta ttgggcctgt tgccccagtt gttcctgttg
    27841 ctcctgtcgc tcctatactt ccggttggac ctgttgctcc tgtatttcct gttgggcctg
    27901 tagcacctgt tactccattt gctccggttg ctcctgtatt tcctgttgga cctgttattc
    27961 catttgctcc ggttgctccg gttgctccag ttagtccggt tggacctgtt gctccagttg
    28021 ctcctgtatt ccctgttgga cctgttattc catttgctcc agtcgctcct gtatttcctg
    28081 ttggtcctgt agagcctgtt attccatttg ctccagttgc tcctttgttt cctgttggac
    28141 ctgttattcc atttgctccg gttgctcctg tattccctgt cggacctgtt attccattcg
    28201 ctccagtcgc tcctgtattt cctgttggtc ctgtagagcc tgttgttcca tttgctccag
    28261 tcgctcccat atttcctgtt ggtcccgtta ttccattcgc cccggttgct cccgtatttc
    28321 ctgttggacc tgttattcca tttgctccgg tcgctccagt tgcacctcta ggccccgtcg
    28381 gtcctgttgg accagtagca cctgttggcc ctgttggacc tacatcttga tataaacttg
    28441 gacctgaaat atcactcata taattaatcc tcctttttta aagttagagt attactctat
    28501 aaatattgag tattactcta tataattata tgaatcgaca atttcttatg tgataatgta
    28561 tctatttgct tatatcaaca tatatgcttt gattcttgaa tttcattact tttttgtaaa
    28621 atcttttaca attattagta ctcttttaca attattaata caattaaaaa actcattttc
    28681 catcaattat ttaaaatttt tttattatgt tataaaaaat agataattaa attgcataaa
    28741 attaatttaa tactacttta attactttgt aaataaaaaa acctcaacac gattaataaa
    28801 aatcatatta aggcatccac aaaattatat aattgcttat ctatcttcat cagtaacatt
    28861 gaatatataa ggaacatttc tataataatc accaaaatca aacccatatc caaccacaaa
    28921 cttatcttct atttcaaatc cacaataatc tggcactaaa tcgacttttc ttctgcttgg
    28981 tttatccaaa agaacacaac attttaaact agcaggattt ttagctttta aatgttttaa
    29041 cacaaaatcc atagtaagtg cagaatcagt aatatcgtct acaaccaaaa catcatatcc
    29101 tgctatatca gtagtcacat ctgaaactac tttaaccctt ccagtacttt cttcattgtt
    29161 tccataacta gatgtagtca taaaattaac tctaagtggt aaatttatat ttctaactaa
    29221 gtctgcacaa aatataaagc taccttttaa tagagaaact accataagat ttttatcttt
    29281 ataatctttt tctataattg ctcctaattc ccttaattta tctttaattt tttcttctga
    29341 atacaatact tcccattttt ttgtttctat atccataaaa agacctccaa ttgtaataat
    29401 ccttcttaat tttataagag attgctcact ttttcaatac tcttattcaa tattagtcaa
    29461 tattattgag tttttatgtt cattcaacaa ttattaacaa tgtatcttat attttaagta
    29521 gttcatatat ccacactaaa ttacatacta tatattttag acaaaaaaag aaaaaacttg
    29581 cactaacaag ttttttctat tatacataaa tttatacata atcatatttt attagagggt
    29641 actaattaaa tttatttatg ctatttttgt tatacctcat aatacaaaac atattataaa
    29701 gtactagtca actttattca tatcattcat tacatttaag attatctcat ctgttataga
    29761 catacctttg tcacttactc tacctaagtt ttgtatactt tgctcaacac ttgaaccaac
    29821 tataccattt agtggtggta caaaacaatc ttgcttagct attatagcac tttgtatagc
    29881 tgcagatgct gcagaagata attttaatgc acaaccagct ttcgcaccgt cacatatcat
    29941 tccacttaag tcagctatca tattttctat agctccttct atttgctttt cagtaccatt
    30001 cataagccaa gagataccag cagttgctcc agtagaagca gcaacaccac atccacatac
    30061 agcagataat cttcctgtat agttcttaat ataaccagtt actagatgag atatagctaa
    30121 agcttttgct agtctttcat cagattgtgg gaatttttta ttataagcaa cgattggaag
    30181 aattgctgtt aatccatgat ttccacttcc atttgaactc ataacaggca tttttacccc
    30241 tgccattctt gcatctgaag ctccagcagt taacatcatt gcataattta taacatcatt
    30301 acctaaaaga ccttcttcta tagacttttt aattccatac ccgacaccaa taccagtttt
    30361 ttgttttaat ccatattcag ccatctcttc attcattttt actccatcta gtaaaaattc
    30421 tatatcttta aaatcaattt cttctacatt ttttattaat tcttgtatag ttactgtatc
    30481 cattaaactt tcagcttttt ctgccgcatc atttgaagct gatacttttg gttcattgtc
    30541 taataatact tctccatctt tttctaagaa cgtaaaatta tcatgtttag ttcttattct
    30601 cactttagca atatgatttt ttccttttaa cacaacttct ataaatactt tctctcttgt
    30661 atcagcaggt gttattgata gtggagtatt atccatataa tcctcagcta ttttaacttc
    30721 ctcttttgtt agagtttcta acacacttaa tccattttca gaatgtccac ctactatacc
    30781 tagagctgct gcaattttta atcctaatct ttcagtacct ggtataccta cacacatacc
    30841 atttttatag atactaggac taactaacat acgattttca actatttctt ctcctaataa
    30901 ttcctttgcc tttgcacatg ctagagctaa ggctactggt tcagtacacc ctactgctgg
    30961 tttaacttct gcctttaaca tttccactaa ttcttttctt atatctctca ttatatataa
    31021 acctcccaat taagtttaga tttagtactc aaataattag ttaaataatt tcttatctat
    31081 atttatagca aaatacatgc catatttttt atatcttttt atatttttag tcttatattc
    31141 tataaccatt ggtatgactg cctctattta tgtatttttt ttatttgtat agagtattta
    31201 tattctttaa aatggagaaa catatgacaa tttcgcaaaa agacaaaaaa aagtctcaaa
    31261 atgagattct atatcatttt gagacggctc aaatatatta ttttttaact tataaaaacc
    31321 attttttaat tcataaaaaa agattctgca aaacacttat agtataagtt taaagctttc
    31381 tttaaagtta tgaatttaga aaaatttaaa ttttatatag ctttttatta tattactatg
    31441 aaattatatt gtatttttca atttttctat ataatgtagc tcttgatatt ccaagtgctt
    31501 tagctgttaa ttctttatct tttttataat ctttatactt atttaaagct tttattatct
    31561 caatcttctc tagttcatca agtggaatta tcctatcgct tttaacttct aactcttcta
    31621 cagaggtatt cttaattttt tgaggaatta cattcattgt gattattgaa gaagaagaca
    31681 tattcacact atattcaata atattctgca actctcttat attccctggc cacttatatt
    31741 ccatcatagc atcaacaacc ctgctatcta tgtcaataac atttttatct aatttttttg
    31801 aatactcttt aaccatatgc ttgattataa gtgggatatc ttcttttctc tctcttaaac
    31861 ttggtaattt tataggtata acatttaatc tataatataa atcttctcta aaagtgccat
    31921 tttgaaccat tctttctaaa tctttatttg tagcagcaat aattcttaca tctattgaaa
    31981 catttgattt acttccaatt ttattaagtt ccttttcttg taatactctc aataactttg
    32041 actgtaaatg caaactcata tctcctattt catctaaaaa tatacttccc ttatgagcta
    32101 cttcaaacat tccaagcttt ccaccttttt tagctccagt aaaagctccc tcttcatatc
    32161 caaataattc actctctaat aaattatccg gtatagcagc acagtttaca gctataaaag
    32221 gattatctac tctattgcta tggttgtgta tggctcttgc aaatagctcc ttacccgttc
    32281 cactttcccc agtaataagt actgttgatg ttgatttaga agctattagg gcttcctttt
    32341 tagaatttct tatagctaca ctttctccaa ttatgttttc caattttatt ctataatctt
    32401 tattcatctt attgtagttt tttatagcat ctcttttatc tataaaatca ataacatatc
    32461 cctttaactc attttttaat actattttat tgatgttgta tattcccttc gaatcatatc
    32521 cgtatctttt ataaaaaaaa ctacaggatt tgtccttctt aaagttattt atagaaggtt
    32581 tctttataaa atctaactcg acaaatatgt ttttgccagt tatactatct tttatattga
    32641 aaagctcttt aaattttgaa ttatatttat caatttttcc atccacatca atagacacaa
    32701 cagctttgtc catattgttt aatagtgtct ctaatttttt cttttctaat tcaagctcat
    32761 aagttttaat ttgtgctttt aatttattag atattaggtc tgccattttg cctaaaaagt
    32821 ttataagacc acttttatta ttttctataa tttgtgcttg ttcttttgta aaggctataa
    32881 ggcctataac accatgagaa tcgccatcac atataatagg acaacacacc tctgcaaact
    32941 ctacacaagc cccactttta tagcattccc tacatatatc actactcttt gggtcatcaa
    33001 ttagtattgt aatctgttct gtaagtgatt ttttaaaagc cgtatattta tctactttct
    33061 cacctatttt gtctatatat actcctgtac ctgcaatcct aaataactcc ttatttacta
    33121 tagttacatc tatattgagt actgatacta tagcttcagc tatattttga atatcagatt
    33181 ttatatattc taaacctatc ataatcatca ttccttattt ttctttagca taagtatttg
    33241 tcttatttct ttaagaatca agtatatctt atttaaagta aattggattg acatcaggat
    33301 tacaaagagc ataacaatta ttattaactc ataatttatt tccatctaaa catctcctta
    33361 taacaaaaga ctaggcttaa cctagtcttt tatattatat ccatatttat attttaactc
    33421 atttatcttc tttttcccac ataaatatat tttaatttat ttgactcttt aatcatacca
    33481 tcaaagttta atcttgaaaa tatattaaat acttcatgtg gatagtgaat ctttgcaaca
    33541 gaaccacaaa atttatgttt aagatagttt aacttaactt ttaaattttt taaatttaaa
    33601 tcctctaata taacctctcc attgcccttt aacactcttt gtatttcaga tgaagctttt
    33661 tcacaatctg taataacatt aaattggtca actattatta tcttgttgaa ctgattatca
    33721 tcaaatggta gaaactctaa cttaccttta actacattgc aattttcatt atacactaga
    33781 gaataacaga catcttcata attactattc tccagtatag ttacagaagt tccaagacct
    33841 ctcaatctct tgcctaggtt tcctatatga ccaacaagca atattttatc acctacatta
    33901 tactcaattg tatccaaaaa acattctaat tcatcagatg tatatttagc tttaactggc
    33961 tgtttattta tctgacataa tatgcttttc acaatgcttc acctcttcat ttcctcaatt
    34021 ataaattatg tacagattac attataataa atggcgagca actattcaag agatatttca
    34081 ttagtatttt tttaaacaat ctttatacat tttaatatga tattatacca aatttattag
    34141 gattttattc ataaaaatca gtctattttt tatgtttatt cattgttatt ttcatccaat
    34201 ttttttattt tcgatatgga tacttcatat gccatttttt tgaccacatt attttcatct
    34261 atttttttct catattctct actttgaacc ctaccccaaa gctgtattct atcaccaacc
    34321 tttagattct ttgcaaattt agcatttcta ccccaaacta ttgatggtat ataatcagat
    34381 ttattataag gtctgtttat agcaactaat aaatcggtta tttcccttcc taaaggtgtc
    34441 ttcctataca caggttcctt acatacatat ccatctaaaa atatactatt agggtcttta
    34501 ttttcctcgt ctatactttt tatttctctg acaaacactg taagcactaa tctattttta
    34561 ttatttaatg ttttgttata tgatcttaat tggcctacga cattgactaa attttgttta
    34621 tcaaggtcaa tatcttggag aagtctttct gatacagtca ttggaagtat atcaaaagaa
    34681 tcgcttagtc tgtttatttt tattttcata ttataaaatt tttctccaaa tatctcatgg
    34741 ctaaattcta gcttattgtc taactcccct cttaagttta ctacgttatt ctcatcttta
    34801 acagctatca tattttatcc ctccacaatt gttaattctt atatttctgg tatcagctta
    34861 tataataaat atatttataa gggatgtatt ttattacaaa tctttgattg ctgtttaaaa
    34921 gtttttattt agttataaat ttcatttaat atttcacttt tataattata aaattattat
    34981 ctttttgtac cacatatact aattaaacta cctatttcag ttttgtatta attataaaaa
    35041 tcctattata taattaataa gtcatatata acttaaattt attcactcta tataagcaac
    35101 ttcaacgaat taaatttatt gaaataattc agatgaatat tgagtcatta taaacttatt
    35161 tttcttaatt ataagttata ttagacttat tacaggttat atttttatta atctgtatta
    35221 atatatgaac atttattgaa cttattgatt ataaaattta aatatttagc tttaaattat
    35281 gctttattct ttaaaaatac aacgtttttt agattaaatc tactaaatca ctatatatat
    35341 tacataattg gcatgttaat tgctttatat taatttagta aataaaaaac tgccctaaaa
    35401 tattaattca atattttaaa gcagtttata atctttatta ttagctgtaa tatttaataa
    35461 acactaagta gtctttaact aagcagtctt tttgttttta tttgcagcca ttatcattgt
    35521 agcagaagta taagctataa ctactattac taatgctttt aatatatcca ttggtagagt
    35581 ttttactata tatgcagcta cgaaaactcc tattaaacca catatagcta ttataactga
    35641 tgctttttta gtatatgcat cttcttttat gaatttcata cttgcaactg gcattaataa
    35701 tgcacaagaa cccataacta ttgggaatgc aacttttgca gacattccta agaaataaac
    35761 cattgccata cctggagcat atagacctat accagctgtc attaaagctc ctagtataaa
    35821 gttacctatt acacctatta ctaatttagc accagtaaga cctaattcat ttcctccacc
    35881 tggtaatacg ttaatccaag gatgacctaa taacattaat attgctgtta atgctaaagt
    35941 tacacccata acaagttgta tttttctctc gtccatctta gctattattc ctgcacctat
    36001 atatgaacca actgctgctg ccacaaccat agatactaaa gtaacaccat ctactgggat
    36061 aattgatgta aatatgaatg cttctgtaac aactggaata gtatgtgcta cgtttaatgt
    36121 accaggcatt tttttatctg gtatatttaa ctttaaagcc tttgacattg ctattgttgg
    36181 agcaaatgac cctattccta atgtatcaaa gaagtctgtt ataaatcctg ttacagcaac
    36241 ttttggagta ctttgttctt ctagtttccc ctctttgttt gctttagcta cgtctttacc
    36301 aaacacaact gcaaagattg ctgcgaatac tacatataat gcctttaaga ttgctaccat
    36361 aatccatcct cctatttttt tattttttca tatattctga atcttatagc ctaaaagcta
    36421 tagaatagca accgacaagt ggctactatt ctattttaat ttcctaattt atttattact
    36481 tatttaagaa taaatccatg tttaagtggg tcttcttcat ctattacaaa atgattaaat
    36541 ccagttatat aagcagaacc agttatttta ggtactacag cattgaaatc tgcaacttta
    36601 gtttcttcaa ctatttcacc tttgaataaa gttcctaata tactttcata tacgaatttt
    36661 tctcctactt ttaattcacc tttagcatgt aaagtagcta atttagcact tgttccagtt
    36721 ccacatggag atctgtcaac ttgaccttga ccaaatataa ctacattttt gtaagtagct
    36781 tctggatgag ttggctcatc atatatttca actaagtcta cagtttttat atgagctaaa
    36841 gttggatgtt gtatttctat tttctcattt attatatctc taagtttcat agctagttca
    36901 gttaatttac cagcattttg aggttcgatt tttaaaccta attgacttgc atgtattata
    36961 gcaaagaagc ttccaccaaa tgatatgtca aatttaacag ttccaacacc tggtaagtca
    37021 acttcaacac cttctttata taagaaagct ggtacgttta agaatgatac ttctttagct
    37081 tttccatcta caactgtaac gtctcctctt attattcctg ctggagcttc cataactaca
    37141 tgagttacag gctctactgc tggaactaca cctgtttcta tagctgctgt catagctcct
    37201 attgtaccat ggccacacat gttaaggtat ccgccaccat ccatgaagat tattccaaag
    37261 tcagcatctg ggcaacaagg ttgagtcatt acagaaccaa acatatcatt atgtcctctt
    37321 ggctctaaca ttatagcagt tcttaaataa tctagatttt cctctaaata ttcttttttc
    37381 tcaggcatag aatttccttt tatgttaggt attccaccta caactattct agtcgcttca
    37441 cctgctgtat gagagtctat agcttgtata cttctgctaa atttcatatc aaattccccc
    37501 ttaggtttat tttgcgtctt tacgcatttt taattttagg aaagccaatg cttttttaac
    37561 atcaacttca tccatgtgat tgtctccaac tacttctgtt tctatgtagc ctgttgtctt
    37621 tttataatca attatggtag ctttatccaa atatggattg gtaattacta atccatctgc
    37681 tcccataata gtaagaccta ctgtaggtat atctgatttt acaaactctg ctatagcatt
    37741 tgtaaaatca atatgctggt ttcctgccat atctaaagtt ataatcactc catcaggtcg
    37801 caatgattca atccatcttc caagtctagt tgctaaaaat gttttccatt cattgtgctc
    37861 tgatgtccca actacaacca ctcctaacaa gtctatgtca gaatcattat ctatcaaatc
    37921 tagtaacggg tctctaaata tatgaagtga tgtgtttttc attgatgtag aaattcccac
    37981 aaaactcatt cccttactta aattttttat ccatgttttc tcattttaag ttttaaaaat
    38041 gccaaagctt ttttagcatc tatttcattt gtgttgtttt ctccaactac ttctgtctca
    38101 ataccttctt ttgatttgtt catatcaact atggtatcca tatattgatt gcttacaaca
    38161 aacttagctt gagtcccatt aaatgtaact ccaactacag gtatatctct ttttcctatt
    38221 tcctcaaatg tgttagcgta gtcaacatgt gagtttcccc aaccatctga tgaaactata
    38281 gctccatcta ctctcatggc ttccagccaa gcggccgttc tttgtcccac aaaggtttta
    38341 ttttcatttc catctggagt tcctactact attactccaa gtaaatctac atccttatca
    38401 ctttcaacta tgtctaaaag tgggtctcta aagtgatgaa gagatgtttc ttttgttgaa
    38461 ggtcctattc ccataatatc cacctctctt aagtcatagc tctaagggca ccatctctgt
    38521 attcattagg agatataatc ataggtacat ttcccatatc tataattgat gttcctcctt
    38581 caaatccact aggttccttt gagaataact gattgtcata catagcacct tgacctgcta
    38641 tttgttttac tatgacaacc ttttttgcat ttggtcgaat tttgtcaaaa tattcacgaa
    38701 cttgagtagc atttcttccg tcttccattt tcaaagacgc tcttatttct tgtataaaat
    38761 catcacatgc tctgaatgct gcaagtggaa gtgttctttc aaaaggtaaa cctcccttta
    38821 aagttacatc tacatggatt atataatctt caactgatgg agttccatat ctcccaaata
    38881 ccatttggtc tttcaaaatt ccttctgaag aaccaaattc atgcatttga tttccatctt
    38941 catctgcacc agttaacatt acatatacac ccgttaaagt atgtgtaata ccctctccca
    39001 gtctgcctaa aacttttgtt gatattggta taatgtccat aatagtatta atttctctgt
    39061 cataatctcc aggttttata atgtctactt ttatgtctgt tattaactct tctctttctt
    39121 ttatttcatc tatacttgat aaatctattg taagtacatc tttctttata gaagtttttg
    39181 aaccgaaatt aacttcattc atatgaaacg gttttataac aagtcttctc attatttttt
    39241 cttccataat atccaccttt gttcttaaaa cccctctcct aaccgtgtag gagaagggtt
    39301 ctatattttc aaatataaat tacattttaa acgtgagctt tatattcgta tggtaatgga
    39361 actatcttac cagcagatgg tattgcaact aattgctcta atgtatctct taatatatgt
    39421 gtttgcattt ctatattatg tggttcacca gcattagcac ccattggaac taatggagca
    39481 actgctctag gagttccgtt ttgtctaact actggaggaa gagctgctat tattattgta
    39541 ggtattccag cttcctctat tgctctctgc acgatcacgg cagttctatg gcaagtacct
    39601 cagccagctg tcataactac accgtctact ccctcttcct taagtttagc agcgatagca
    39661 ggaccagttt cttcagtgaa gacatgttgg tttcctccac cacccatgaa tgcatagtgc
    39721 ataggagcta ctcctttgat aaatccttct gcagctaatt catgtaatct atctataggg
    39781 aacatacagt ttatatcttt gtttacatct ccattgtcat atccaccatg tgataccatc
    39841 atttcatcaa ctgtagctgt attaggtata gctctaaatg tagtatctcc tgctagatta
    39901 aatctcttgt cagattttaa atgaacacca gctgctgtag ctaaagctat agtcatatct
    39961 tttaattctt tagttacagg agtccaaact ggaggaggtg ttattggaac gaatatttca
    40021 gattgaagtc cttgtactgt tgtaaggctc atatatttcc tctcccttct attttcaaac
    40081 gattatttat tatttttctt cttgttttga ttgcatttct gcttgttttt ctttttctct
    40141 cctaatattt tctacatact cttgattagg ctctggactt aatacctcag gatagcttag
    40201 catgaatcca acttcttgac ctatcttaag ttcgtagtca gttataagca ttctattagg
    40261 tattttaaat tgtccaagtc taccttttag gtctatagtc actccaccgc ctttgatgtc
    40321 aactataata ccttcataaa aagtctctga tgagatatat tttaacctat ccattataat
    40381 tctacctctc tatactgttt taaacttgga tctaaattta tgttattgtc taaatcttag
    40441 ttagtatcta aattttagtt ttcgtctttt tcgtatattt ctcttctctt cttactcatt
    40501 ggtagagatt gctcattctc ttctaatact attttttctc ctgtaacttt ttctatagct
    40561 tctatgttat ttaatttaac atttggattc cattttcttt caggagcttt aacttctaca
    40621 ccttctatag catttttaag catagccatt attctaacag catcttctgg agctaaagtg
    40681 ttgttagata atatttcatt ctctatacct tgcttagact tattgttgtc taccatgtga
    40741 gtcatgtatt tattaccaac aactagagca ccttgaacag ctgagaaact tacaccaact
    40801 actggtatac ctctcatacc tatttgctct atgtgagatg cgaaatctat atggttgttt
    40861 ccgaaacctt cagtagttac tacagctcca tcaacttcca tagcttcaac taacatacct
    40921 aatctcttag atacatagaa tttctcagca tttacttgtg gagaaccaac aaatacaaca
    40981 cctactaaat ctatttcttc gtcttccata gctcttatta ctaatggctc tctccagtaa
    41041 tgtcttgaca tttcttttga tgcaggtcct atacaagtta atgcatggat accaccatct
    41101 aatacttcaa gtggagataa tacaactggc atgtttccta agtcaacgtt tggttgagct
    41161 cctaatgtac caactggctc aactggtaat attaggttat catgcattgc accttgtccc
    41221 attatttctt ttataacaac aactttcttt ttaccttctc ttctgtattg ctctattact
    41281 tcttcatcaa ctactaaaga gttgtcaact ttctttaatg cttctcttat ttcttgagtt
    41341 acatagtcaa atgcacggtg agcagctaaa ggtcctggtc tttccatgtt agttcctgct
    41401 tttattgtta cttgaccttt gatgaatatt tcacctttgt cagcagcacc tggtctaccc
    41461 cacattatag tagtatttaa ctcaccttct gaagaaccaa attcacctat ttgaactcca
    41521 tcttcatctg taccagttaa taccattaca gctccatcta taactctagt tatacctgaa
    41581 cctaattcgc cttcttcttt agtagcgatt ggttgtatat ctaatacagc ttcactgtaa
    41641 gtttcatatt tatcaggagt tattatttct aacttcatat ctacaactaa ctcttgagtt
    41701 tcattagctt ctttacatat ttcttctgcg tttctgatgt ataaagtagt tccttctatt
    41761 ttagtttcat cagcaaattc aactttatct attttaaagt gttttttagt taagcttctt
    41821 aaaagtttct tttcttcaac ttttggagct acttctgctg ctgcacttgc cactggagca
    41881 gcaacgtttg ctactggagc agcaactcct gcttgaccag ctactgataa aggtatctct
    41941 aagcttatgt tcttaccttc acctatgtgt attttaactg tttcacctgc aactcctgtg
    42001 cttactggaa ctactgctgc ttctgatact ggagctgctt cttctactgt ttcttctact
    42061 acttcttctt tagcctctcc tgctttgtat ccttctacgt tatctgtagt cattggagaa
    42121 agtgcatcta aagtttcttt taacttagct cctagtactt gacctatagt taatccattt
    42181 tctggtattg ttaataatcc agaatcctct aagtctgcaa atattgctgg atcttctaag
    42241 ttttctggcg ctataattgt tcccgcttca aatctacaac aacaaactgc tgggtcattt
    42301 gcatgggctt gagctgtttc taaagttatt gacatagtat aaaatacctc cttacattat
    42361 tttcttattt attattttaa atctcaattc ctcaacaggt aaccccttaa ctgagaaatt
    42421 aagtaaatta aaacctacat aatttgttat gattaatatt atttttttct cacttaaagt
    42481 cactttatat tataagcaat tcctatgcca acttattaac aaaaagattt tttctataat
    42541 agattcttca tctatcgcta tttttagcca aatattatat aaatatttat ttaaaatatg
    42601 ttattttttg tatttgtaaa cattttctca aacaaaaaaa gtgtcaatta atcaacactt
    42661 tttcattttt tttaacacat ctatatacac aacacttata taagcgttgg ctttactagg
    42721 ttttaaatta aagtttatca ttttgtcaaa atttagtcat gtgtcatatt ttagacacat
    42781 ctattttgta ttggtctatc ttataataca aagttgttct gggtatattt aatatcttag
    42841 ctgctttagc tttatttcca ttagacattt taagtgcctt ggttatattc tttatttcaa
    42901 ttttttgagt tgccttcgtt aaatctaaag gatactcttc ttcttcagta ctattatttg
    42961 tagaatccat catataactt ggtacatcat ccacatctat tttagaattt tgtgacaata
    43021 caacaatatt ttcaatcgta ttttttaatt ctctaatatt acctttccat ctataattct
    43081 gaaatatatc tattacatct ttgctaattg taagaacagg cttattattt tgtttacata
    43141 tctcctctaa aaagctatga accaataatc ctatgtcttc ttttctctct ctaagtggag
    43201 gaatctttat ttcaacaaca tttaatctat agtataaatc ttctctaaat ttttctgcct
    43261 ttaccatttt actcaaatct ttattggtag ctgatattat tctaggattt atttttattg
    43321 tggtatctcc accaactctt cttatttctt tttcctgtaa tactctaagc aactttgctt
    43381 gcatactaag aggtaaatcg gcaatttcat ctaaaaatac agttccatct tttgcaagtt
    43441 caaatatacc tattcgaccc tttttacttg ctcctgtaaa cgcacctgat tcatacccaa
    43501 aaaactcact ttcaaataac tcatttggta tggcactaca gttaacaggt ataaattgac
    43561 cttttctctc actataatca tgaatagctc ttgcaaatac ttctttacct gtaccacttt
    43621 cgccccaaat aaatatgcta ctatttgttc tagccacttg ttttgcaatc gctttcgatt
    43681 tttccaattt ataactttta cctaaaatct tatcaaaact acctttagat aaatttttga
    43741 cttcgttctt tagatatttt aatgtatcat ttactttttc aagttcaaga gctaatatct
    43801 tagcttctgt aacatcaact tctgtacata caacaccgat aaattcttct ttataaaata
    43861 taggatttgc atgaactaat gcatacatat tttctttttt atctgtaaaa tacaagtcac
    43921 tcattccaac tttactattt agtacttttt ctgaaatagt attttccaaa aattcattca
    43981 tcggtcgacc aactatatct tcagacttta tatcatacct ttcctccata aacttattcc
    44041 acaagataac aacaccttcg ttatttatag cacaaattcc ttctttaatt tgacctataa
    44101 tatacttcaa agttaatcca taatcttcta aatgcatata tagatagtcc ctaatgtgct
    44161 cttgttttag aactccaata attttattat ctctaagaac aggaagaatc cctatatttt
    44221 ttaatatcat tatatctcta cattcatcta aggttaaacc ttcgttgaca tatattacat
    44281 ccttcttcat tataagtttt actggttgac cttcatactt tttatgcatc tcataaagat
    44341 tatgtatatc cgtcatggat ataatgcctt ttaattggtc agatgaatct attaccatta
    44401 gagtttttgt gttgcttttt atcatttctt ttattgcatc ttcaattctt gtgtcttcgt
    44461 ctatagttgt aaattttgta tccattacct cttctacttt ttttacttct ggtatcgaaa
    44521 acataaagtc ccccctttat agtagtacct catcaaaact tatttatata tatttattct
    44581 agcatattat aataattatt tattaaataa ttattattaa aatttattaa gtaattagca
    44641 tttaaattta tataatcact ttaaaattta taattaaatt tatacaaatg aaatcaaatg
    44701 aattataccc ttagtatatt attttttcaa tactattttc accttcaaaa catttttcta
    44761 ttcatcaaac aattaatgaa tgcctgtatc caatcataaa tttaagaata taattaaaat
    44821 atatattttt aaacaaacta tatttgcata ttatatttac ataaaaaagc cccaaaatga
    44881 cttataaatc aatttgagac tttttatttt aaacttaata ataatctaga ttaatttctt
    44941 tttattttac attgatacgc taatccacaa attgcaatga cactaataaa tacatatgac
    45001 attagctgag tttcatcacc tgtttttggt gattttatta ttgtatcatc tcttgatagt
    45061 tctggagttt tttccactgt atcacttact gatggtactg gtggatttaa cactgtatcc
    45121 tctgttggtg gcactggtgg atttagcact gcatcctccg ttggtggcac tggtggattt
    45181 aacactgcat cctccgttgg tggtactggt ggatttatta ttgtatcatc cattggtggt
    45241 actggtggat ttattactgt atcatctatt ggtggtgatg gttttggctt atgtccattt
    45301 gtatcatctg ttggtggcac tggtggattt acaacatcat ctgttggtag acctatatta
    45361 aagttaactt cttgaccatt ttttttctct attgtataac ttaataaatt ttgcttacta
    45421 gatggtaatg aagatgaatg gtctttttgt cctaaatcaa gcactgcctt ataatattga
    45481 atagcttttg gaaaaatata atcataagat acagcagaaa tattagcttt ggcatctggt
    45541 tttacaacac tcttaagttt aaatttctgt tgtgttgaaa tttctacttc acctgtacct
    45601 attatattac catctccatc ataagcagtt actccatctg gtagtcttaa ctttactaca
    45661 ccactataac ctactaaggc tacagcttct gtttcataag taccatcttc atttttttca
    45721 aagttaatct tagttaaacc attactcaaa ctaggttttc ctgtatcatt tggctttaac
    45781 tcttcacgac ctgcttttaa caatgcttct aaataagctt ttatatttat agctacttct
    45841 ttcttcttct gatactcagc tctttcctct tcagtttttg ctctttctat aagtttatca
    45901 caaaggttta tagaaccttg atactgctta agtctttctt gttcatccat ttgtccaaga
    45961 ataatccaaa ttgcactttg agtagctgag taagtcatca attcattttc ttctccacct
    46021 aaattaaatt gttgcttata tccaaatcca tcaaattcag aacctgcata tagtatagat
    46081 ttaactgctt cttcattttg gttttcttga ccattaaaat cttctgctgg taaatatgga
    46141 ctctttccat tactacttgg tggtcttaaa tgattattgt aacaaaatgc agtttgctct
    46201 ccatcttcac cttctattac atatttgtcg tcttttgagc ttgctcctac aagacccttt
    46261 attacatagt caaaagttaa agtctttgta atatcatcaa atcttaaatc atgataaggt
    46321 ctatctgaac catctatttc ttttagtaaa caagaatttt ttacttttgc ttttttgtca
    46381 taccataagc taataaatga gtcttctgaa gtaaaaactt ggttaatttc tgattgttca
    46441 tctgccttta catcttcttc atcttcccaa aagttatata ctattttatt tatttgagat
    46501 aaagtcatat caagaatttt atcaatatct tgttttaaac tcatttcctc tataactatt
    46561 tcttcttcca atggtttttc ttcttctgga gtttgctcat ctaatggttt ctccccatct
    46621 ggcatctgac catctgatgg tttctctaca tctggcgtct gactatctga tgtcttctct
    46681 ccatctggca tctgaccatc tgatggtttc tccccatctg gtatctgacc atctgatggt
    46741 ttctctccat ctggtatctg accatctgat ggtttctctc catctggtat ctgaccatct
    46801 gatggtttct ccccatctgg tatctgacca tctgatggtt tctccccatc tggtacctga
    46861 ccgtctgatg gtttctcccc atctggtact tgactgtctg atggtttctc cccatctggt
    46921 acctgaccgt ctgatggttt ctctacatct ggtgtctgac catttgattg ttcttctcca
    46981 ttaagtattg tatcattgct agtcacttcg tcagcataag atactattgg tgtaaatgca
    47041 gtaaacacca tactaaaagc aattactaaa ctgattatac ttgatttttt aatttttgtt
    47101 ttcaaaattc tcctgatact aaaaacatat attgtattca cttaatataa ttaaaattat
    47161 taaatattat atgaatctta ttcaataata catctttagt gtgttcaatt agggtcgtta
    47221 ctcctaacca gttctcctat gaacttctta atatttctat taagcacaga ctatatcatt
    47281 acctaaccat aaattaggta ctccccattt ccccacactt gtaggtacga gatttctctc
    47341 tagtcgttga agtttcttct tttcgaagct tacctgctga ttatccattt tcaaggcact
    47401 taggatttaa ccttatgcta tctaactaat tttttctact ttcgtaacct caagtgctta
    47461 ttaaatgcac actctatcac acttatcctt atttcatgat tatgttgtgg tttagttagc
    47521 tttaggaact tccagcaatt aaaggagttt tgggcagata tctgccactc tacactcgtt
    47581 atgaatgcag ggagcatttc aaaaatatat ttatatctat cttctattaa ttacttattt
    47641 atgtatttat tttatatatt tttgacaatt aaaagttact cctatttata tttatagcag
    47701 gttgcactac taactccata tatcttttaa gtgcccaggt ttaagatata cttcacagtt
    47761 tctataaata ggttttttaa tttttctttt ttaacttttt aagtgcactt ttttaacaac
    47821 tcaataaaat ataaattagt atcctagata cttatctaat aaaagacaaa aattagtttt
    47881 tataataaaa aagatactat agagtatctt caaatatcac aaaaaccttt tactctatcg
    47941 gatagttgac ttaatataat atgcatatta atacaaacca catacttgag tatcattatt
    48001 tttgtccaat attaattgta atagctatta ttatcattat acaatatcta gcatattttg
    48061 tctatttttt ccatttattt tacaatatat atattgccct caaaatacat taaaaaacat
    48121 ccttagaagt aacttttaag ttttacttct aaagatgtct aacttctatt tatctattat
    48181 tttattttta atcttacgtc accttttatt cttcttatta atgggtgatt aactgttctc
    48241 attacatggt cagtaatatg atatacacct aattttaatt ttggtgcaga acccattact
    48301 gtgttactac agtcagtaca gtttcctatt aatataactt ctgctccagc tttcttaaat
    48361 tcaatacatt tttgagcttg tcctggacag tgtcctggat tttcacattt taatgcacct
    48421 ttaatttctt gagcttgttt acatttttga actgatacta tatcagtaac tcccatttta
    48481 gttgctatat ctctcattct ttcttcatta ggtccacatg cacaaactaa aagacccatt
    48541 tttctctttt cattaacaaa tggcatagta actattatac caccactagt tttagttatt
    48601 ggagcatcta aagtagtagc tttaccagtg aaaggtcctc ctaggattat ttcaccatat
    48661 tcacctttta ttccaccagc tctttcaatt aattctccga cagttgttcc aattggcaca
    48721 tcttcaaaca ctatagattc ttttccactg tttaattgac caactacagt tatattttta
    48781 gatataactg gtcttttttg ctcaacagcc tctactattc tagttagtgt ttctacgttt
    48841 attactactg cattagcctc tgaaggtaat tgagttggct caagtaattt tcctagaacg
    48901 tctcttacta ttgctctctc ttctcccatt gggtacatat caactagttc tgcaacttct
    48961 atgttgtctc ctggctttat tacttttttg aaagcttcta ttgcttttgg atgcttactt
    49021 tttattgcta gcataccttt tccagcatta gtaacttcca tagcatattt tataccttga
    49081 taaactattt ctggtctttc ttctatttct tttatattat gagctaataa aggttcacat
    49141 tcagctgcat ttgctaatat tgttccacca tttaaatcta tgtttaattt tacatgagta
    49201 gggaatcctg ctccacccat tcctactact ccagcttctt taattaagtc aagtatacct
    49261 tctcctttta atggttcaaa agtatcttct tgtacttcat cagcttcaat tactatcgct
    49321 tggtcattta tttcacttat ttttccacta acactggcat aaatatttgc ccctaatcca
    49381 gttggttctg cgattagagt tccttttttt actacatctc caacacttac tactggctta
    49441 tctggaccac caacgtgttg ttttaataaa atagaatata gtttgttcaa ctagtacgcc
    49501 cccttaagta tattaatttt tttataagct gttcaaataa tgttaatgct atttttctat
    49561 tataaggtct taacatattg tttatttcta tagaacatat cgcttattaa caaagcaatt
    49621 tctatgccaa cttttaaaca aacttatacc ctttggaaat actgtcttac agcattcatt
    49681 aattcttcta aatttttaaa cccatcaaaa aaaagtcaat tattagacac ttttttaatc
    49741 aatttaatct ataattattg atattgcata catgtataaa aagtgtcaaa aaatagacac
    49801 cgtcttttta tcgacacttt tctaacatat actatataat ttatcacaac tttacttaaa
    49861 atatacattt attacttatt aattcttagt tttttgcact ccattggaat caactacata
    49921 attatcttta tacaacaaat cagatatttt gactagactg tatccatctt tttttagtcg
    49981 acttattata gtatccaaat agttatctac atcatttata ttagcatgga ataaaactat
    50041 tgaaccaggt tgagattctt ttattacttt ttcaattaca tgatttggtc caagctcctt
    50101 ccagtctata gaatctacat cccacttaat tactttataa cctaaatcac tacaaatgtc
    50161 catagcttta ttgtttacgt ccccaaaagg aggtctaaat aaattagtcc tttttcctgt
    50221 tatattatat attttttctg atgtagtttc tatctctttt gttatgtctt cttttgatat
    50281 ttccttcaaa ttggcatgag tatttgaatg atttccaatt tcatgtcctt tttcatctat
    50341 agcttttaca atttcttcat tatcatccac ccaactacca actaaaaaga aggtagcttt
    50401 tacattatgt ttatctaaaa tttttaaaat atcatccata ttatcagttc cccatgctac
    50461 atcaaaactc aaagctattt ttttctcatt tgtatcaact ttatatatag gcatttttga
    50521 attgtcagat ttagaacata tgtattttat tccataaaca aaaacaaata ttaacactaa
    50581 taataatgaa aaaaataaaa cactatatat caaagcttta gtccttttaa cttctctttt
    50641 catactattc cccctttgaa ataatttctt ctatatttaa aattt
//
LOCUS       DAYAOY010000018        49778 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYAOY010000018 DAYAOY010000000
VERSION     DAYAOY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.018"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(229..1245)
                     /gene="tsaD"
                     /locus_tag="ACGI6B_001118"
     CDS             complement(229..1245)
                     /gene="tsaD"
                     /locus_tag="ACGI6B_001118"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFG9750582.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(1251..1727)
                     /gene="rimI"
                     /locus_tag="ACGI6B_001119"
     CDS             complement(1251..1727)
                     /gene="rimI"
                     /locus_tag="ACGI6B_001119"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFG9750583.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(1717..2433)
                     /gene="tsaB"
                     /locus_tag="ACGI6B_001120"
     CDS             complement(1717..2433)
                     /gene="tsaB"
                     /locus_tag="ACGI6B_001120"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFG9750584.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(2433..2885)
                     /gene="tsaE"
                     /locus_tag="ACGI6B_001121"
     CDS             complement(2433..2885)
                     /gene="tsaE"
                     /locus_tag="ACGI6B_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFG9750585.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(3024..4064)
                     /locus_tag="ACGI6B_001122"
     CDS             complement(3024..4064)
                     /locus_tag="ACGI6B_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9750586.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(4393..4998)
                     /locus_tag="ACGI6B_001123"
     CDS             complement(4393..4998)
                     /locus_tag="ACGI6B_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9750587.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(5123..5240)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5300..6466)
                     /locus_tag="ACGI6B_001124"
     CDS             complement(5300..6466)
                     /locus_tag="ACGI6B_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9750588.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(6614..8755)
                     /locus_tag="ACGI6B_001125"
     CDS             complement(6614..8755)
                     /locus_tag="ACGI6B_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFG9750589.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(8899..10006)
                     /gene="prfB"
                     /locus_tag="ACGI6B_001126"
     CDS             complement(join(8899..9930,9932..10006))
                     /gene="prfB"
                     /locus_tag="ACGI6B_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFG9750590.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(10021..12696)
                     /gene="secA"
                     /locus_tag="ACGI6B_001127"
     CDS             complement(10021..12696)
                     /gene="secA"
                     /locus_tag="ACGI6B_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFG9750591.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(12828..13379)
                     /gene="raiA"
                     /locus_tag="ACGI6B_001128"
     CDS             complement(12828..13379)
                     /gene="raiA"
                     /locus_tag="ACGI6B_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFG9750592.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(13519..13644)
                     /locus_tag="ACGI6B_001129"
     CDS             complement(13519..13644)
                     /locus_tag="ACGI6B_001129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750593.1"
                     /translation="MWIEIGVKIALNKQKQELLTVLSTLSTGVCNNINPHKYQCN"
     gene            complement(13932..14606)
                     /locus_tag="ACGI6B_001130"
     CDS             complement(13932..14606)
                     /locus_tag="ACGI6B_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFG9750594.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(14609..15661)
                     /locus_tag="ACGI6B_001131"
     CDS             complement(14609..15661)
                     /locus_tag="ACGI6B_001131"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HFG9750595.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(15710..16669)
                     /locus_tag="ACGI6B_001132"
     CDS             complement(15710..16669)
                     /locus_tag="ACGI6B_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HFG9750596.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(16762..17283)
                     /locus_tag="ACGI6B_001133"
     CDS             complement(16762..17283)
                     /locus_tag="ACGI6B_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HFG9750597.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(17294..18628)
                     /gene="celB"
                     /locus_tag="ACGI6B_001134"
     CDS             complement(17294..18628)
                     /gene="celB"
                     /locus_tag="ACGI6B_001134"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFG9750598.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIQFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(18667..18993)
                     /locus_tag="ACGI6B_001135"
     CDS             complement(18667..18993)
                     /locus_tag="ACGI6B_001135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9750599.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(19017..19325)
                     /locus_tag="ACGI6B_001136"
     CDS             complement(19017..19325)
                     /locus_tag="ACGI6B_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9750600.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(19466..21379)
                     /locus_tag="ACGI6B_001137"
     CDS             complement(19466..21379)
                     /locus_tag="ACGI6B_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9750601.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(21711..22493)
                     /locus_tag="ACGI6B_001138"
     CDS             complement(21711..22493)
                     /locus_tag="ACGI6B_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFG9750602.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(22496..24715)
                     /locus_tag="ACGI6B_001139"
     CDS             complement(22496..24715)
                     /locus_tag="ACGI6B_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFG9750603.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(24979..26076)
                     /locus_tag="ACGI6B_001140"
     CDS             complement(24979..26076)
                     /locus_tag="ACGI6B_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750604.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(26391..27584)
                     /gene="metK"
                     /locus_tag="ACGI6B_001141"
     CDS             complement(26391..27584)
                     /gene="metK"
                     /locus_tag="ACGI6B_001141"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFG9750605.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(27721..27824)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            28013..28522
                     /gene="yyaC"
                     /locus_tag="ACGI6B_001142"
     CDS             28013..28522
                     /gene="yyaC"
                     /locus_tag="ACGI6B_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HFG9750606.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(28633..29061)
                     /gene="fabZ"
                     /locus_tag="ACGI6B_001143"
     CDS             complement(28633..29061)
                     /gene="fabZ"
                     /locus_tag="ACGI6B_001143"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFG9750607.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(29077..30096)
                     /locus_tag="ACGI6B_001144"
     CDS             complement(29077..30096)
                     /locus_tag="ACGI6B_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFG9750608.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(30293..30553)
                     /gene="spoIIID"
                     /locus_tag="ACGI6B_001145"
     CDS             complement(30293..30553)
                     /gene="spoIIID"
                     /locus_tag="ACGI6B_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HFG9750609.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(30651..31319)
                     /locus_tag="ACGI6B_001146"
     CDS             complement(30651..31319)
                     /locus_tag="ACGI6B_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFG9750610.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(31385..32449)
                     /gene="spoIID"
                     /locus_tag="ACGI6B_001147"
     CDS             complement(31385..32449)
                     /gene="spoIID"
                     /locus_tag="ACGI6B_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HFG9750611.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(32536..33789)
                     /gene="murA"
                     /locus_tag="ACGI6B_001148"
     CDS             complement(32536..33789)
                     /gene="murA"
                     /locus_tag="ACGI6B_001148"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFG9750612.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(33810..34547)
                     /locus_tag="ACGI6B_001149"
     CDS             complement(33810..34547)
                     /locus_tag="ACGI6B_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HFG9750613.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(35003..36298)
                     /locus_tag="ACGI6B_001150"
     CDS             complement(35003..36298)
                     /locus_tag="ACGI6B_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFG9750614.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(36749..38581)
                     /gene="glmS"
                     /locus_tag="ACGI6B_001151"
     CDS             complement(36749..38581)
                     /gene="glmS"
                     /locus_tag="ACGI6B_001151"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFG9750615.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(38848..40194)
                     /gene="glmM"
                     /locus_tag="ACGI6B_001152"
     CDS             complement(38848..40194)
                     /gene="glmM"
                     /locus_tag="ACGI6B_001152"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFG9750616.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(40364..40921)
                     /locus_tag="ACGI6B_001153"
     CDS             complement(40364..40921)
                     /locus_tag="ACGI6B_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9750617.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(40922..41674)
                     /locus_tag="ACGI6B_001154"
     CDS             complement(40922..41674)
                     /locus_tag="ACGI6B_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9750618.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(41674..42753)
                     /locus_tag="ACGI6B_001155"
     CDS             complement(41674..42753)
                     /locus_tag="ACGI6B_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9750619.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(42782..42997)
                     /locus_tag="ACGI6B_001156"
     CDS             complement(42782..42997)
                     /locus_tag="ACGI6B_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9750620.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(43040..43717)
                     /locus_tag="ACGI6B_001157"
     CDS             complement(43040..43717)
                     /locus_tag="ACGI6B_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9750621.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(43707..44786)
                     /gene="buk"
                     /locus_tag="ACGI6B_001158"
     CDS             complement(43707..44786)
                     /gene="buk"
                     /locus_tag="ACGI6B_001158"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9750622.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(44808..45710)
                     /gene="ptb"
                     /locus_tag="ACGI6B_001159"
     CDS             complement(44808..45710)
                     /gene="ptb"
                     /locus_tag="ACGI6B_001159"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HFG9750623.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(46014..47195)
                     /locus_tag="ACGI6B_001160"
     CDS             complement(46014..47195)
                     /locus_tag="ACGI6B_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFG9750624.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(47192..48031)
                     /gene="cdaA"
                     /locus_tag="ACGI6B_001161"
     CDS             complement(47192..48031)
                     /gene="cdaA"
                     /locus_tag="ACGI6B_001161"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFG9750625.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            complement(49015..49088)
                     /locus_tag="ACGI6B_001162"
     tRNA            complement(49015..49088)
                     /locus_tag="ACGI6B_001162"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49052..49054),aa:Arg,seq:tct)
     gene            complement(49098..49172)
                     /locus_tag="ACGI6B_001163"
     tRNA            complement(49098..49172)
                     /locus_tag="ACGI6B_001163"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49138..49140),aa:Gly,seq:gcc)
     gene            complement(49183..49259)
                     /locus_tag="ACGI6B_001164"
     tRNA            complement(49183..49259)
                     /locus_tag="ACGI6B_001164"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49223..49225),aa:Asp,seq:gtc)
     gene            complement(49265..49340)
                     /locus_tag="ACGI6B_001165"
     tRNA            complement(49265..49340)
                     /locus_tag="ACGI6B_001165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49305..49307),aa:Val,seq:tac)
     gene            complement(49346..49419)
                     /locus_tag="ACGI6B_001166"
     tRNA            complement(49346..49419)
                     /locus_tag="ACGI6B_001166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49385..49387),aa:Gly,seq:tcc)
     gene            complement(49434..49508)
                     /locus_tag="ACGI6B_001167"
     tRNA            complement(49434..49508)
                     /locus_tag="ACGI6B_001167"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49473..49475),aa:Glu,seq:ttc)
     gene            complement(49516..49591)
                     /locus_tag="ACGI6B_001168"
     tRNA            complement(49516..49591)
                     /locus_tag="ACGI6B_001168"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49556..49558),aa:Met,seq:cat)
     gene            complement(49607..49692)
                     /locus_tag="ACGI6B_001169"
     tRNA            complement(49607..49692)
                     /locus_tag="ACGI6B_001169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49656..49658),aa:Leu,seq:taa)
     gene            complement(49698..49772)
                     /locus_tag="ACGI6B_001170"
     tRNA            complement(49698..49772)
                     /locus_tag="ACGI6B_001170"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49738..49740),aa:Asn,seq:gtt)
BASE COUNT        14805 a         9492 c         5353 g        20128 t
ORIGIN      
        1 acccactttt taaaataatc tttctataga aaaaaataaa cttgaagcaa atctaagcca
       61 aagacaatct gtatatccta aaatttgttg caattcattg gaatataaag attataaata
      121 gttttacttc aagttttaat atatactata tttattttta ttatatttaa tttcacttct
      181 acttactttt ttgaaacaat acttttatat ttacaaattc atctaactct actgatttat
      241 ctttaaattt ggaactgcat ttaaactcat atcatgcgtt ttcccattta taaaattata
      301 atatcctgca caccctatca tagctgcatt atctgtgcat aaaattagag gaggatactt
      361 aacagtaata ccattgtctt tagcaagctc tgttatttta gctctaagcc ccgaattaga
      421 agcaacccca ccagaaagag ttatgatatt gtatcctttg tcttttactg cttttagtgc
      481 tttagtagat aaaacctcta ctacagcctc ttggaaagat gctgctacat cttcaactac
      541 tatttcttca tttttcattc tttttccatt tagataattt aatacagaag attttaatcc
      601 actaaaacta aaatcatagc tatcttcttc taaataggct cttggaaatt ctatagcatg
      661 tttatttcca ttcttggcca gattatcaat aataggacct cctggatagc ctaaattcat
      721 agctcttgaa atcttatcaa atgcttctcc agatgcatca tctcttgttt ttcctaaaat
      781 ttcatacttg ccataatctt ttacttcaac caagtgagta tgacctccag atactattaa
      841 tgttataaaa ggaggtttta aatccttatg ctctatatag tttgcactta gatgcccttc
      901 tatatgattt actcctacaa gtggaatatt caaagtatat gcaagtgctt tagcataaga
      961 tagaccaacc aataaagcac ctactagccc aggtccatat gtaactgcga tatggtctat
     1021 atcattaaaa cctatatttg ctttgtctaa agcttcttga acaactatat caatattttc
     1081 cacatgtttt cttgaggcca cttctggaac tacaccacca aattttttat gtgtctcgat
     1141 ttgagtagag attatgtttg aaagtacttc tctaccattt tttaaaactg atgcagctgt
     1201 ttcgtcacaa ctactctcta tagctaatgt tattatatca ctcataaact ctacacctct
     1261 tttaattggt tccacattat aattgcatct tctttattat cactgtagta ttcttttctt
     1321 attccaccca ttttaaatcc atattttcta tataaatttt gtgctatttt atttgatgct
     1381 ctaacttcta atgtcataga tgcaatatta ttatctttac ataaatcaac tatgcctttt
     1441 atcaacttgt cccctatttt tttccctcta tagtcactat gaacagccac attatttata
     1501 tgcccttcat caagtataag ccaaattccc acatatccga caatttttcc atttaactta
     1561 gctactatat accttgctac ttcattagac aactcttttc taaaagactc ttttgaccaa
     1621 taatcttcaa aacaattttt ttctacttca aaaacttcat ctatatcttc tgtagtcatt
     1681 tcttctattt ttatatcatc tactattagt tttgtattat cttccattat ttagcctctt
     1741 cattttttca tcatattgta cttcagcttg agattttctg atatacatag gatttatcgt
     1801 ataacaagta tgtacatcta tatttttatt atatttattt aatgctaaag agcataagct
     1861 tcctgcgtta gatacattat tagctggtga aggtattttt atatttttta tattctctaa
     1921 cttgtcttta tatttgtata cagcttctcc aactattata aattcttcat tagttgatac
     1981 aatttcatct actaaagcat caaactctac aacatcaaca ccatctaatt ctatcatctc
     2041 tccattttca aacttgtatt tagctgaata aacttgattt ttctgtgcat ctaatataca
     2101 acatatcttt ctatcacaga aatttatgtt atttgctaga gattccaaag aatttactgc
     2161 tattattggt atattattta catgggccat agcttttact gtagccatac ctattctaag
     2221 tcctgtaaac gaacctggac ctatacatac agctaataaa tccatatctt ttatacttaa
     2281 atcactcata ctcaacatat tttctatcat tggcataagc ttttgtgaat gagttttttt
     2341 gttgtttact gtaaactcac atattaagtt atcatcttca acaactgcta cacttgctgc
     2401 catagaagac gtatccatac ctagtatttt cattatttaa tcaactcctc aactatctct
     2461 ttataaaact ctccttttgg agttaatatc atttctcttc cttcatcttt atatctcaat
     2521 tctatattca aatattcttt tggaagtata tcttctatta gattagccca ttctattata
     2581 cagattccat ttgaatttac atattcatca taaccaatat catacatttc atcactacta
     2641 cctattctat aaacatcaaa atgatacaaa ggtatttttc cttcatattc atttattata
     2701 gtaaaagtag gacttgttat ataatcttct atccctaatg aatctgccaa actttgtgtc
     2761 attgtagttt taccagctcc taaatctcct actaaacaga taacactgcc ttcttttaaa
     2821 agtttaccta atttatatcc tatttctcta gttttatttt cattttctaa atatattttt
     2881 gccatcaatt catctccatt ttgatataat cgtcatgtga atttatatca ctgcttaatt
     2941 aattatagta taagcctaga ttattataaa gtacttgtct ttatcttttt ttatctttca
     3001 ctattgatgg ctgtatctac accttatata caatcatcaa tagcaatttt tacatactct
     3061 tttgctttat ggttttgctt caatgcttct ttacatattc tctcagattg ctcttttaca
     3121 tacttaagag acttatagtt ctcttttact gcttcaatac acattttttc tgtttgattt
     3181 tttatataca taagcatttt tccattttgt cttacaatct ctatacatat atcttctgta
     3241 tacttatcta tgtaacttag tacatcttta atattatatt ttttatttaa atatcttata
     3301 gcatttatac atatttcttc tgtcttattg ttgacaaatt gtatacttct tccatcttgt
     3361 ttgacagctt ctatacatat tttatcagtt tgtttattta catattgtaa tgccatacca
     3421 ttttgtctta cggattctat acacattttt tccgtttgat tttttacata ttttaatgct
     3481 ttataatttt gcttaatggc ctctatacat aattcttcac tctgttctct aacgtatgca
     3541 agacacctgc catctttttt aattgcttct aaacatatcc ttcttgtttg agtctctaaa
     3601 gcctttagta tacttacaac atctatattg gaagaatgac ttattgcttt tataataagc
     3661 ccttctgttt tatttcgtac atacttaagt gcattacaat ctttttttat agcttcaaga
     3721 cacaagtatt ctgtttgttc ttttacatat tcaagtgctt tataattata ctttattgct
     3781 tctaaacata attcttcggt ttgatttttt acatactcta atgccttgta attctgcctt
     3841 attgctaaaa tacacatacc atcagtttga ttttctatat tttttaattc acgataatcg
     3901 tattttaccc tttccatatt ttcaatatat tcattagtga tatactttaa agaatctcct
     3961 aattttgtat cataaatacc aatatgaata tctttccaat tactattaaa ataataccct
     4021 ttatcatcta aaaataattt ttttattttt ttagataaat gcatgaatat acacccctat
     4081 cattattaaa ttagttatta ttaaaagttt gattattaat ctttatagtt ttttaataat
     4141 aactttattt tttgtttatt ataaattcgc tctgtatatt gtagatacct ttttcatatt
     4201 ttaatagtta atcgttttaa ttcttccttt attttaaaat ttaaatttgt ctaaatattt
     4261 cttattttat atacattttt tgataaatta ataaagccat aatatattta atatatcaat
     4321 cacaatatag gactgaagtt ataaataaat tatggctatt caatacattt aattttatat
     4381 tttcttgtct aattatttct ttattatatt tcccattttt ttataaagtg gcaatgttac
     4441 taaagatatt ataacagcct tcagcacatt aaatggagct atcatccaaa ttacaaaagt
     4501 gaataaatca tgaacttgag gatttatagc tgaacctaaa cctattatag catctagtcc
     4561 cataagttga ccataaagtg gcaacattac aaaataattt actaatgaac caattatagt
     4621 catagatatt gttcctaata caaaaccaat cagtacacct tttaaatctc cacttctctt
     4681 atatgaataa caaagtatca ttacataaga accaccaatt aatatatttg caaattctcc
     4741 tattcctcca gttgttgttg ctgttattgc ttgtaacaca ttttttacta caactattcc
     4801 aaatccaact aatggtccta gagacattcc tccgaatatt gctggaatat ctgataagtc
     4861 aatttttaag aaatctggaa atatacctgg tattggtgct tgaataaaca ttaataaata
     4921 agatattgca gataaaattg acattactac taaagttctt gttgaaatca tattgcctct
     4981 ttttgctgtg ttttgcataa ttattttccc cctaatcaat atgtttatat tttttaggaa
     5041 aaacaaaaaa ctcgaagacc ctatcttcga gtttttatgg atatagttta aacaaactaa
     5101 aataaactta tttatcttcc ataaatatct tctctcatcc agactttact gtcggctttg
     5161 gaatctcacc aaatcaacca cctaagtagt tcgcgggcta taccgccggt agggaatttc
     5221 accctgcccc gaagatcttc tttgattttt taattttcct tacactctta tattagttaa
     5281 ttttttaata attgtcaagt tattttttct catacactac tttgccattt attatagtgt
     5341 aaagtacatt actttgaatc tctagtggag aattatccca aattactagg tctgcatctt
     5401 taccaacttc tatagagcct actctatctt ctattcctaa agccttagct ggatttatcg
     5461 taattgattc tattgctttt tcttctttca taccatgttt tactgcaata cctgcacata
     5521 ttggaagata ttgaactggt attactggat ggtctgtcat tatacataca tcaagacctg
     5581 catttgaaag tattcctgct gtattaaatg tcaaatttct aagctcaaat ttagaccttt
     5641 ctgacaaaga tggccctact attacaggaa acccttcttc cactaattca tctacaataa
     5701 gatgaccttc tgtacaatgg tctaatgtaa gctttaaatt aaattctttt gcaattctta
     5761 ttgctgtaaa catatcatct gctctatgag catgtgcctt aaaaggaatt tctcttctta
     5821 aaactggtat taaactttcc atttttatat catattcagg tttttcacag tcttcatcat
     5881 catgactctc atacatatca atatcctcta aatattcttc tgcttttttt aggttttctc
     5941 taagtaaagc agctatagcc attctagttt gaggagattt atcatcctga ccataacagc
     6001 tctttggatt ttctccaaaa gcaattttag atgcaactgg attttttatt accattttat
     6061 caattctttt tccacatgtc tttatagcta tacattgacc acccataaca tttgcacttc
     6121 caggggttgt acaaacagaa gttattccac cttcaaaagc ttctttaaaa gttctatcca
     6181 ttggattaat tccatctata ggatttaatt gtggtgttat tgggtcagtc tcttcatttc
     6241 catctgctcc ttcaaaccct ataccatctt cccataaacc taagtgagta tgagcatcta
     6301 taaatccagg aaacacaagt ttattactag catctattac ctctacatct aaaggagcta
     6361 tcaaatcttc tccaatttct attatttttc cttcatctat aagtatatct ccatgaatta
     6421 tcccatttgt tatggtattt attttaccat tttttataaa aatcattcat tttcctcctg
     6481 attttcaaga ctatcgttct tattttaata tattttctta ctatatactt ttacaaattt
     6541 ttaacattta tacactcgag taatcttatt tacaaatata gaatctaagt actataacca
     6601 tacataaact tatctatttt ttcattgata aggcaactct ttttttgttt aagtcaattc
     6661 caatgacttt tacatctact atatcaccta ctgtaacaat actcataggg tctttcacaa
     6721 aactattact catttctgat ttatgaacta atccgtcatt ttttattcca atatctacaa
     6781 atgcaccaaa gtctacaaca tttcttattg taccttttaa agtcataccc tcttgtatat
     6841 cttcaatttt taagacatca gttctaagta taggttttat accttcttct cttgggtctc
     6901 ttcctggttt tttgatttca gctatgatat cttttaacgt aacttgtcct acttccagtt
     6961 tttcacttaa ttccctcaag ccaatttctt ttattttttc atctatttca cctatattct
     7021 tattctttac atcatctaaa gaatatccaa ttatctcaat cattttttta catatatcat
     7081 aactttcagg atgcacacct gtattatcta gtggattttt accctctaag attctcataa
     7141 atccagcaca ttgagtaaat gcttgaggtc ctagtctttt tacttttttc aattgtgctc
     7201 ttgacgtaaa atctccattt tcttctctat atgctattat attttttgct atagctttac
     7261 ttattcctgc aacatgttct agtagggagt atgatgcagt atttaaatct actccaacac
     7321 tatttacaga atcttcaaca actccatcta gtacttcttc taatctcttt ttgtttaaat
     7381 catgctgata ttgaccaaca ccaatacttt tagggtctat ttttacaagc tctgctaatg
     7441 ggtcttgtaa tcttctagct atagatatag ctcctcttat tgatacattt atatctggat
     7501 gttcctcatt tgcaagttca gatgctgaat aaacagatgc tcctgcttca ctaactatca
     7561 catactgcac ttcgctatct atttctttta tcatttctga tacaaaagtt tctgattctc
     7621 tagatgcagt accatttcca attgaaatta tatctatatc gtatttttca atcaatcctt
     7681 ttaaaacttt ctttgctccc tctacatcat tttgagggtc tgttggatat actgttgtat
     7741 aatctagtag tttaccattt ttatctacta cagctatttt acatccagtt ctaaatgcag
     7801 ggtcaaatcc cataactact ttgtctttaa ctggtggttg aagcaataaa cttttaacat
     7861 tctttccaaa tacacttata gctctttctt gagctatttc tgttaaatga tttcttattt
     7921 ctctttctat tgatgggaaa attaatcttt tataagaatc ttctattgaa cttacgattt
     7981 cttctttatt tttgaaattt ttatcattta catattcgtt tattatgtaa tttaaaactt
     8041 tatcattatt tatttccaac ttaaccttta ggaagctttc tttttctcct ctgtttattg
     8101 ccaaaactct atgtggagcc atacttttta ctgcttcgct ataatcataa tacatatcat
     8161 aaaccgattt ctcatcagta gcacttttgc taactatcat accttctctt aaagctaatt
     8221 ctcttatata ctttctaatc ttagcatcat ctgagactag ttctgcaatt atatctctag
     8281 caccttttag agcatcttca atagaaagaa cttctttctc ttcatcaaga taattctttg
     8341 cctctatttc tatattatct aaattattct ctagtattga caatgctaaa ttttctaatc
     8401 ctttttcttt tgctattgtt gctctagttc ttttcttttg tttatatgga gcatatatat
     8461 cttctacttc ttgtaatgtt tttgcttttt ctatattttg tgttatttca tctgttaatt
     8521 tcccttgttc atctattagt ctaactacat catcttttct actttgaaga tttctcaaat
     8581 acattaattt ttcataaaat tctcttagtg taacatcact catttcgcca gtcatttctt
     8641 ttctatatct agctataaat ggtattgtat ttccttcatc aataagtttt aatgtattat
     8701 ttatttgttc atctctaaga ttaaactctt tttttaagat ttgatttata tccaatttat
     8761 tcactcctaa tgttagtttt tcataagtat aacaaaacag aacgaaggct gagggttatc
     8821 gtcctcagcc taagtgctgt aagtgcatat taagcactaa ccttcttata catatatctt
     8881 aattacagtg catatttatt aaactatttt attcattttt aagtattcat taataaacaa
     8941 gtctatattt ccattcatta cactatctac atttccaaac tcagcatttg ttctatggtc
     9001 tttaaccaat ttatatggtt gaaaaacata tgacctaatt tgactacccc aagttatttg
     9061 tgagtatttg ccttgtatat cttctatttt ttccttttgc tctaactctt ttaattctat
     9121 caatttcgcc attaaaagtc tcatagctct gtccttattt agatgctgag acctttcatt
     9181 ttggcactga actactacac cagtagggat atgtgttatt ctaactgctg aatcagtcgt
     9241 atttacatgt tgccctccag ctccagaagc tctatatgtg tctatcttta aatctgatgg
     9301 attaatttct acttctatat tttcatctaa ctctggtgtt acatctatag atgcaaatga
     9361 agtatgtctt tttcctgatg ggtcaaatgg agatattctc actaatctat gaactccttt
     9421 ttcactcttt aaatacccat aagcatttgt tccttctaca agaattgtag ctgtctttat
     9481 ccctgcttca gggtctgata ttatatctaa aagcttaact ttataaccct tactttctga
     9541 ccatcttata tacattctca aaagcatttg agcccaatct tgagcatcta agccaccagt
     9601 accagcattt atagataata tagcattatt tttatcatac tctccattta gaagagtctt
     9661 tattttcatt tcagataatt tttcttccat tgattctatg gacttttcta tatctctttc
     9721 tacagaatca tcatcttctt ctaaacctat ttcaattaat acttctatat cttctaataa
     9781 gctttttaag ctttcatatt cttctaatgt ctcttttaaa gatttatttt cttgaagtac
     9841 tctttgagcg acttcattat cattccaaaa atcttgttga ttcatctttt cttcattaat
     9901 ttgtatattt ttcatcaatg aagctatgtc aaagagaaac cctcaattct tctaaatttg
     9961 aagccatctg ctccatacta tgtctatact cttgtaactt taacatttaa tcctccgaga
    10021 ttatcttcca caacagtttt tatatttttt accacttcca caagggcata agtcatttct
    10081 tccaaccttt tcatcttttt taacagtttt acttgttgga agtgttccat ctgatggaga
    10141 agatagattt tcaacatcta caactgcttt tctttccact ggagtttcta ttgttatatt
    10201 aaataaatat cttacagtat cttctttgat atgtttattc atttcatcaa acatatcaaa
    10261 accttccatt ttataagcaa taactgggtc ttgctgacct acagcacgaa gaccaatacc
    10321 ttggcgtaat tggtccatag catctatatg gtctatccaa tggttatcaa cagcttgaag
    10381 taagattact ctctcaactt ctctcattct ctcatagcca acttgttctt cttttgaagt
    10441 atatatcttc atagctattt cataaacttt ttctgttatt tcaactggat taagtttttc
    10501 tatttctggt atttctatgc taccttttgg caagaacaga ttatacatat gttctttgaa
    10561 tccttcttca tcaaatcctt tatcttgtgt ataaagagta actgcttctt ctattataga
    10621 atgagtcatt gattgtattt gttcttgtaa atcttcacct tctaatacac gttttctctc
    10681 tgcatatatt atttctcttt gtttattcat aacatcatca tattgaagta catgctttct
    10741 tattccaaag tttttacctt cgactttctt ttgtgctcct tcaatagact tagataatat
    10801 tctatgttct atcggcatgt cttcttcaag acctatttta tctacaatac cagatattct
    10861 atcactacca aacaatctca taaggtcatc atcaagacct atatagaatc ttgaactccc
    10921 tgggtcacct tgacgtccag cacgacctct caactggtta tctattcttc gagattcgtg
    10981 tctttctgta cctattatag caagtccacc tgcttctaca acttgttttt gttcttcttg
    11041 tgtttgttgc ttaaactttt caaatagttt ttcatactct tctctagctt caaatagaat
    11101 ttcattacct tttacaggta taccttctat tggagtatct actctattta ctatttcttc
    11161 tttaaatcca tttcttctca tttctctctt agttaaaaag tctggatttc cacctagaac
    11221 tatatctgta ccacgaccag ccatattagt agctattgta acagctccaa gtctaccagc
    11281 ttgtgctatt atttctgcct ctttatcatg atgcttagca tttaatacct cgtgctttat
    11341 gccctttttc tttagtattt gtgataataa ttctgatttt tctattgata ctgtacctac
    11401 aagtattggt tgattgactt tgtgcctttc aattatttct tgtgctacag cattaaattt
    11461 acctatttca cttttgtata cacagtctgg taagtcttcc cttatcataa gcttattagt
    11521 aggtacttgg aaaacatcca ttttgtagat tgctttaaac tcttcttctt ctgtttttgc
    11581 tgtaccagtc atcccagaaa gttttttgta cattctaaag taattttgga atgttacagt
    11641 agctagtgtt ttagattctc tttgtatttt aagaccttct ttagcctcta ttgcttgatg
    11701 taaaccttct gaatatcttc taccaaacat aagtctacct gtgaactcat ctactattac
    11761 tatttctcca tctttagcaa cataatcaac atctttcttc attattacat gtgctcttaa
    11821 agcttgattt atatgatggt ataattctgt atgagatata tctgttatat tatcaacatt
    11881 aaaatacact tctgcttttt gtatacccga tgctgttaga gatacagcct tatctttttc
    11941 ttctaattca taatcatctg gttttaaggt taaaacaaat gtgtttgcat ctgaatataa
    12001 gtgagttgat ttgtctccag gtccagatat gataagtggt gttctagcct catctattag
    12061 tatagagtca acctcatcca ctatagcata atttaatcct ctttgaactc tttgctcttt
    12121 gtgtattacc atattgtcct ttagatagtc aaatccatat tcattatttg taccataagt
    12181 tatatcacaa tcatattgtt cttttctaac ttttggattt tgaccgtgta taataacgcc
    12241 tacactcatt cctaaaaact catagatttt tgccatttgg tctctatcac gttttgcaag
    12301 gtaatcattt actgttacaa catgtacacc ttttccagta agcgcattca aataaacagg
    12361 agcagttgca accaaagttt taccttcacc tgttttcatt tctgctattc ttccttgatg
    12421 tagtactata cctccaatca tttgaactct gtagtgtcta agtcctaaca ctctcttaga
    12481 aacttctcta actactgcaa aagcttctgg aagtatatca tctatacttt caccatttgc
    12541 taatctttct ttaaatatat ttgtcatatt tttaagttcc gagtctgcca tagattcaaa
    12601 tttaggttct aatgaatcta ttatatcaac agttttattg aactttttta attctttttt
    12661 atctgccatg ttgaataaat tatccataaa gctcatattt tacatctctc ctcgttcttt
    12721 attgtactat tattcatttt accataaaaa ctatactttt aatattaatt taaaaagcta
    12781 tcgataattt atttatcgat agctaatatg attaaatcta aattgcttta ttcatgttct
    12841 attataccat atccaccatt attacgctta taaactactg ctatttcatc tgaatctatg
    12901 ttcttgaaca tataaaaatc atgtcctatt aattccatct gtaatacagc ttcttcttca
    12961 ctcattggct taacactaaa tttcttacgt ctttgaatta ctaattcttg attttcatcc
    13021 ccataattct cttgttcatc agctagattt atctctgcat cttggaatct tatactcttg
    13081 ttgtctttat gtttatcttg taatctcttt ttatattttt ttaattgttt acttaattta
    13141 tcatatacaa catctatagc tgtgtataaa ttttcctgca tatcttctgc tctaattata
    13201 ggaccattta cagtagctat tgttacttct attttttgtc ttgcttttct cgcacttact
    13261 gtaactttta catcagtgtc tggatgaatg taattatcta acttacttag tttagtttcg
    13321 attttatttt ttattgcatc agttaattcg atttgttttc cagatataat tatgttcata
    13381 aatacatctc ccttcaatta tgccagtata agaaaattta tactttaata atatattcta
    13441 cacgaactct cataatcctc tttttattat ttacttttat gattttttct attcaattga
    13501 tagaattatt tatcattttt agttacattg atacttatgt ggattaatat tattacaaac
    13561 acctgtggat aatgtggata aaactgttaa taactcttgt ttttgtttat ttaaagctat
    13621 ttttactcct atttctatcc acaaaaatct ccattaattg tttattaata tgtgaattca
    13681 tgtcataaga tggtaatttt aggaaattat gtgtattata tgccaatata cctttataat
    13741 tttttaataa atataaaccc tctaaaacct ccataatcta tcttttcttt cattatttat
    13801 atttaaaact aattaaaagt tataaagcat actcaacaat aatttcatat ttaataccta
    13861 ttaacattca tatacccaaa ttataattaa cttactctct ttatttaaac taagtatttt
    13921 atattcatat actatcttga aattatattt acgagttgtt taagcttttg tctatctata
    13981 tcttcaaaag gactgttatt tattctaatc gctgtaccaa aatgaaaatt acttgcctta
    14041 gttaattcct ttatcttttc tatattatta aaattaagac ctccacctaa aagaattttt
    14101 atgtgattag aactaagcat catattcttt atcatttgta tgttatcaac tatacttcct
    14161 ttccctcctg atgttaatat attcgtaatt ttatcataat ccttcaaaat tttaacacta
    14221 tctattgtat ttgattcatc tattgctcta tgaaatgtaa catcaagatt atcacaacat
    14281 ttcaataaaa catttagatt tttctcatct atactattgt ttttatccaa tacaccaaat
    14341 acaactccat tagcaccaat ctctttaacc acacttatat ctttttgcat tatacttata
    14401 tcttctttac tgtatacaaa acttttcgca tgatgtctta tcattacgtt cacaggtatc
    14461 tttacactgt taacaacact ttctataaga ccaaaacttg gtgtcaatcc accttcagtt
    14521 aaagcactaa ctaactctat tctatctgca ccacaatctt ctataatttt agcgtcttct
    14581 actgacattc ctattatttc taacataact actcctttgc taattcatat attgcatttg
    14641 caaatatctt tgcatttaat attatatctt ctatatccat gtattcatca ggattatgag
    14701 ctattccttt ttgtcctgga aatgaaggtc caaacggaac tatatttggc attacttttg
    14761 catacgtacc tccattagtt gttacgggag ttccatctaa tgatgtaacc ctttcataag
    14821 ctagctgaag ctttttaaca agattactct ctttatcaaa atatacagga ttaaaattag
    14881 aaattacctc tatatcgata ctatttggaa aactagcttt tatttcatcc aatacatctt
    14941 tcaatttata actaaaatcg tattttacct ttatatctaa tatagtatca ctattttcta
    15001 tattcagctc tttatatgta acaagcacat tttcagacat attttctata aaacctaggt
    15061 atacatcttc ttcataattg attttacttt ttattctaat ctttgccatg cctttttcac
    15121 catatacaac aggatattta caatctggtg taaatcccat aataggtgct ttttcttttt
    15181 ctaaataata tggtatatct tcaaatccac tttcttcatt tgtaccaaat attattctaa
    15241 ctctcttatc taattttaag tttaattctt taatagcata taatccatat aaggcagaca
    15301 ttataggtcc tttattgtct agtactcctc gtccataaat tcttccatta gtttcttctc
    15361 ccttataagg ctgatgcttc caaccatctc cttcatgtac aacatctaca tgtccaatta
    15421 cacaaacata atcatcacta tctccatgct ctgcataacc aatataatta tccaagtttc
    15481 taaccttaaa tcccaatttt tctgatattt ctaatgcttt attaagtgcc tcccctactt
    15541 tttcgccaaa agggaagcca ttttctgact cccctttgac actaggtatt tttacaatat
    15601 ctataatatc acttaataaa tcttctttta actcatctat tttattactt atgatattca
    15661 taaaaatctc cttattataa aactctattt taattatgta accactactt tactcactta
    15721 cttctattgg tgcttttatt ctagcctcat atgcttccat ccactcattt gatataacct
    15781 tatgtatagt ctttccatct actctatttg aaatatatac agtaggagat tcttctttag
    15841 ttactactga aaaagtaaaa tcagctatat ttgtagctac tccccattca cctttattat
    15901 tcatggcaac taatgataaa tcaccagctt ttcctcttct tttgataagc tgtttatcaa
    15961 gttcatttac tgcaatatca catgcttctt gtggatgctt cccttctttc ataagtctaa
    16021 ctatctcata agatatacat cccttcatta aatcttctcc caaaccagta gctgtagctc
    16081 ctcctatact actatcaaca tagaatcctg aaccagatag tgcagaatct cctattctac
    16141 ctcttttctt cataaacaat ccacttgttg atgtagctgc acacattttt ccagttttgt
    16201 ctaaagatat cataccaaca gtatcatgac cagcatatgg gcttaatcct ttgtctaaag
    16261 tctctttttt tctcttttta taatgtaact tcgctctatc agtcaacata ttttttcttt
    16321 caaatccatt tttatgagca taatcttcag cacctattcc aactaaaaaa ttatttacct
    16381 tctcataact taacttccta gctatgctga caggattaga ataatctctt ataccagcaa
    16441 ctgcgccaat atctaatgtg ttaccatcca taaatgcagc gtcaagttct acctcacaat
    16501 tttcatttgg aagtccacca tatccaaccg atttatagaa tggatagtct tcaactcttc
    16561 ttacagcaag ctctacagca tcacctgctt ccatgccttt ttctaaatgt tctgatgcct
    16621 cagttattcc ttctagggcc attctccatg tcgctatcat tccccacatg ttaaaattcc
    16681 cctccttaaa ttatatagat tttactaatg tatgcttatt ataaatagta taatatttaa
    16741 acttgctaat aattaatact cttattgata acaaaactct tctttcatca aagttatatc
    16801 ttctttattt gctattgctt ttgccaattt acatatgtta tttaatgaat cattaagacc
    16861 aattccacct ttttttggag tcttaactat acatatgtca tttttatatt cgtttatcac
    16921 atctatattt tcttctgaca tagctgcaaa agctggtata tctgttttat catttatatt
    16981 tttagacagt attgctgaca tcttagaaaa gttttcgtaa ttaaagcttg gtccacagat
    17041 tactacatca gggtttaatt tttttaccat agctatcatc ttactcgtta cttcttcttg
    17101 attatttaca aaaaattcat ccccacaaaa taaggtagct attaccttag attcatttaa
    17161 aaattgctcc atcattacac ctggtcctaa tgggcttgat tttccagcag gaggtatatt
    17221 acttttctct tttcctccag ctccagcttg tatttggtcc aatatcataa ctattttctt
    17281 cataatcttc ctcctataat actaaatcat caaaagatat atcatcatct tcctcttcaa
    17341 cagtttcatt ttcttctctc aagtattgct tatctagcat ttttataaat tgtatataca
    17401 taaatattcc tagtataaga agtactaatt gtaagactgc accttgccat cctgttgtta
    17461 aaaaaccaga gaatataatt ggagttgtcc acggtaactg aactccattt gtaagaggca
    17521 ctaatcctgc tgacatacag aagtaagcta ttattatatt tattgttgga actattgcaa
    17581 atggtatcaa tagtattggg ttaagcatta ttggtagacc aaatatcaat ggctcattta
    17641 taccaaatat tcctggtaaa attgatagtt ttcccattga ctttatacgt ttacttttac
    17701 atattaataa catagctatg actaatgata gtgtactacc tgctccacca aatgtcgcaa
    17761 acatatcttg gaattgacct gttattatgt ttggtatttt tgaaccagct tgaaaagcag
    17821 ctaagttttc agctgataga atttttaata ttggattata cacagcccca acaacactac
    17881 ttccatttat tccaaagaac cagaataaat gtaagaatat ataagcgatt gccatagcac
    17941 ctaaactatt tcctaatttt aataatggca tttgtaaaaa cttaaatata aattcatgta
    18001 tacttccata tggtgtagct tcaaaagcta tcttaactaa aaagaaaact accataacta
    18061 ttgcacttgg tattaatgct gcaaatgatt ttgatacagt tggaggaact ccatctggca
    18121 ttttcaatgt ccatcctttt tttacaactg cagcaaataa ttttacagag gttattgctg
    18181 ttatcatacc tacaaacatt cccttagaac ccatccaacc tagtggtata cctgttacca
    18241 tgtaaactga ttctgttcct tctggtgtat atggtattac aaatggtgtt attataaaaa
    18301 aggcaactat tgcaactgct gctgatgcta ttgggtctac atctatttgc tttgcataag
    18361 aatatccaat ccctaaaact gcaagtagag tcattacttc aaatgttgct acagttggtt
    18421 gttgcaattt tacagcccaa tctggaccaa aaatttgacc tacaaattca ttccatcctt
    18481 ttataggaaa atttgctact aataaaaata atgaacctat tattaataat ggtgatgata
    18541 ctaaaaaacc atctctaatt gcaattaata atttattttt acctattttt tcagccaatg
    18601 gcataagtat tctttccaat ctattcatct atccaccctc atttcctatt attttttact
    18661 ataattctac gcttgtttag attttattag ctttatagca agttttaata ctttttcccc
    18721 attcatagtt ccataatcaa cttcattgat tatatctata agttttcctt gagcttcaaa
    18781 ctttggtttt aactccttta gcatgtattt aacttgtggt cctaataata tacaatctgg
    18841 atttttttct tcaactattt tgtcaatttc tgctatcgga aatgcctcta cttctattgg
    18901 taaattgtgt tcatctgcta ccttctgcat tttatctgca agtaaacttg ttgacatacc
    18961 aaagctacaa aataaatata cttttctttt cattttttaa atccccctta atttttttat
    19021 tttaattttt gaatttcttt tcttaacata atcatttctt ttataaggtt tctctcactc
    19081 atacaagtca ttaaatggtc ttgagagtga atcaataaaa tagatatatc tgatttatct
    19141 cccccagctt ccttttgtat aagttcagtt tgtactttat gtgcttttaa aatttcttca
    19201 tttgcatttt caattaattc atctgctttt ttaaaatcgt tttcctttgc atagtcaagt
    19261 gcttcataaa gcatagattt tgcatttcca gcatgaatta taatctttaa aactatttct
    19321 tccatttttt cctcctaagt tgcttctcta cttttagatt atttatacct tacaaacata
    19381 taatatctta taaaaacaaa aaaacatgca caactttttc catcacatgg aaaattttgt
    19441 gcttatccat agttcactat gaattttata taagaatatc ttttaccttt tcaatgtcac
    19501 atttacttag aatatctagc atattttcat cctctgttaa attagatatt tctttaaata
    19561 attctctaaa tacttttcta tcatcatctt ttttatacgc taacaaaagt ataatcttta
    19621 tatcctttcc atttctaatt tttattggtg tttttaacct tgcaaaacat aaaacagatt
    19681 gttttatata tttcatgtct ccatgaggga ataatattcc ttttccaaca ttggttggat
    19741 atgacttttc tcttgttata atagattgta aatactcttt ttctgcataa tgtttttcaa
    19801 ttaataaatt agttaaatat gttatagctt cataaatatc atcatgttct aactctctta
    19861 ttaaataatc agacatcatt aatctgttca taagtttata tgaagtgtct ttttctttta
    19921 ttttgtttgt gaactcttta agtttattct catcctcatc agtaaataat ggtgttgtta
    19981 taaaaattgg tttttggata ttttcatact cttgaaatgc aattataaaa tctatagtct
    20041 tattaaagtt atctcttttt aaatcttcct ctcttaatgt ttcaataatt tttatgttat
    20101 caaatctctt ttctatcttt acttttaata gcatacttac tccaaaacta aatggacata
    20161 caatacttgc ctttttattt gattcccttt tgctgtattt tctttctaga gaggtttgaa
    20221 agtgaagtgt aagataacca atttcatctt ccgttatttg ttttcctttt aattcaattt
    20281 ctattgaact tgatattact gtaaataaaa aacaaaattt agtttttatg ttatctaaaa
    20341 gtggattttc taagtaaaca ttgcttttca tttgatgcat tgtaacattc aaatgacata
    20401 ttaattgttc ataaagtaat atatcattat taaaatttat tcctgattct tctgatacta
    20461 aatttataat tttttttgta taacttgcta attcttcatt tagatataaa gatttcttaa
    20521 tattattact atttatttgt ttgttcatac ccaatattaa agattctata aacaattttt
    20581 cttcataatt taatactatg gataaattag atttcaattc atcttctata tccaatataa
    20641 gcatttttag cttaagctct ttcttagtat ttgatacttt taaagaattt cccagcctta
    20701 ttcgtattat atttataagc aaaaatagca tcaattgctt gaatgatata tctgtaagtc
    20761 ttatatccat atcctcttct atagtaagca ttatgttttt tacaattatt atatctacta
    20821 tatggaagta ctctatttca tctatgttta atctgtattt atctatatat ttaaataaga
    20881 atgatactaa cattattctt atgtctttct catccccttc aatatatata ccactgcctt
    20941 ttttagtcga gatatttaaa ttataattat ttaatgctac tgataattta cttatttctt
    21001 ctctgacaat atttttattt atgtacaact tattagataa atcatttaaa gtaacttttt
    21061 tattattaca aagtagtaaa tttagtatct caacttgctt atgaagctca ctatttactc
    21121 ttttaacttc acctcgcaac ttctgattta ccagtatttt gtctgttcca tctccactaa
    21181 atttaatccc aatattaggt tttcttttga tataagcatt acaaaaagta gataaccatt
    21241 catcaatata tttgcaatca tttcttattg tcctttctga acactcaaaa tcattggcaa
    21301 tcttattaac atttataaac tcttctttta atagcaatgt tgttaataaa ttttcttgtc
    21361 ttttattcaa acatatcacc tctttatgac tcaacctaaa tttattatat ataaaaaaga
    21421 gctatacata aaggtatgct agctctcttt taatatgata tttagctgac ctggtttttg
    21481 accccacact cgcctactgg agggttcgct tgggttcgcc gcactactac tctctctcga
    21541 ttttattatc ggtaggtctt acttctaagc cgcaccatga tcattaaccc aatttttagt
    21601 gttaacttac gtggatccct ttgcctgcga ctggcttgga ctttcaacca cagacctaaa
    21661 tatcacttat tcattttaat attttaaact aaaattgtca atatataaga ttattccata
    21721 atataattat catttgcttt tgtaagtaga gtcaatacaa aaaccttatt agcacctgat
    21781 aactttaaaa gtttagaaat ttcgtttgtt gttagaccag tagtaaatat atcatctatt
    21841 aaaagtatat ttttattatt tattaattta acattttctt taacaacaaa tacatttttt
    21901 aattcttctt ttcgttcttt cttattcaat ttatatagca ttcttgtata tttttttcta
    21961 cttatgcaat ctaataatgg aatatttact attttactta aattaaatgc tattttttgt
    22021 gcctgattaa aacctctttt atttagcctt tttttatgaa gtggaacaaa taatatataa
    22081 tcaaatttta tgttttctaa atataatttt tctttcatta tttgagctat atacttagcc
    22141 ataaaggttt tttggttata cttcagccct aacaccatct tttttgtcac atcattgtat
    22201 tcaatacaag atattgattt atcaaaataa aagtctttat taaaacaata actacactct
    22261 tcaataaatt ctttttcaat tgaatgacgt attattggtt ttccacactt catgcaacca
    22321 tcttgtataa aattcatttc tttaaaacaa cttttacata ttgaataagt atttgttttc
    22381 tttatggatt tatcacaaat tatgcaactt atattctcag gatatataaa atccaaacat
    22441 ttattaatca tatttttaaa attcttaata aaattatcat tgcatatttt catatttatt
    22501 ctatcaacaa cccttcttgt ttaaatttat ttagcttata tcctaaattt gaataccttt
    22561 gattaactct attatttttt atcatatatt caagatattt cacatctcca actaatacaa
    22621 ctaatttttt tgctcttgtt actgctgtat ataataaatt acggctaagt aacataggag
    22681 gagcccacgc tattggcaat actacaactg gaaattcact tccctggctt ttatgtattg
    22741 ttgtacaaaa actatgatca atttcatcta attcatcata taggtatgat acaattttag
    22801 tctggtcaaa taatatataa atagttttct tatctttatc tatgtgataa acatatccta
    22861 tatctccatt atagatacct tctccacttt ctttttggtc ttctgtttcc catttttttg
    22921 tataattatt ttttacttgc attaccttat cgccaactct aaataatctt ttagaaaaat
    22981 tttcttcaac tttaaatttt tcttttttgt taagatactt ctgtaactct atatttagat
    23041 tagtaactcc caattcacct tttctcattg aagataaaac ttgtatatct tttaatttat
    23101 caactttata aaatttaggt aatctttcat taaccaatcc tattatttca tttaaaattt
    23161 cttcattagt tgattttctt ataaaaaaga aatctttacc ttttgtattt agataaagtg
    23221 gttctccatt gtttatctta tgggcattta caattatcat actttcttgc gcttgtctaa
    23281 atatttcatt taatttaact acatttatga tattagaatt aatcatatcc ttaagtacat
    23341 ttccagcacc tacagaaggt aattggtcac tatctccaac taaaattact cttgttccta
    23401 actttatagc tctaagtaaa ttgtacatta agataatgtc taccattgat acctcatcaa
    23461 caattattac atctgaattt attgggtctt cttcatcctt aaaaaaagtt aaatcatcat
    23521 ctgttgaaaa tcccatttct agcaacctat gtatagtttt tgcttcttta ttagaagttt
    23581 cactcattct ctttgctgct cttcccgttg gagctgccag agtcacactt ttaccattat
    23641 tttcaaatat atctatgata gcattaatgg tagttgtttt accagttcct ggtccaccag
    23701 ttataattaa tactccacta ttaacagatt ctttgactgc taaaatttgt ttttctgcta
    23761 acttaatttt cttatcttct tctaaaacat taatctcgtt atctatatct atttttaagt
    23821 cttcaaattc atactgtgat aattttacta tttgactaca tactccattt tcagctaaat
    23881 agtatggcat aaggtatata agattttcat tgtttacttt ctctatatgt attttttgat
    23941 tataaactag catcattaca cactctttta ttatttctcc atttagctcc aataattttt
    24001 ccgaatcttg tattagaaca tgctctggta aatatgtatg cccattactt aaagatttat
    24061 ttaaagtgta taagattccc tgacaaattc tatctttaga gtttttatct ataccaattt
    24121 tacttgctat actatcagct actttaaatc caattcctct tatatcttca gcaagttggt
    24181 atgggttttt atttataact tctattgcac tgcttttata ttttttatat atttttaggc
    24241 agtaatttgg tgttattcca aaaggagata gctgtattat tatatttctc aattctctgt
    24301 cttcttcata actttttact atttgtttta cttttttact ccctatacct tctacttctt
    24361 gtagtttttc tggtgaattt tgtatcacct ctaacgtatc tacaccaaac ttatcaatta
    24421 ttctttttgc cattttttca ccaataccat gtatcattcc agaagataag taaacataaa
    24481 ttccttctaa ggatgatggt gttactggca taaaactatt cacttcaaat tgagtcccat
    24541 aaatcttatg atttacccac tttccttcaa cttctatgct ttctccaatt gctaaagtag
    24601 gcatacatcc tacaaccaca atttcatcat tttcatttac caggtgagct attgtataac
    24661 cattatcttc gtttttaaag actatttcac ttatcattcc ttctaatttt tccattttta
    24721 tctcctaaaa atactaataa tcgctccatt taaatgttag tataatttta tatattaaaa
    24781 ctatgataat cgtagaaaag ttttatattt ttatacttaa cgtaatttcc aactattata
    24841 cattttatca gaaacaaaaa aaatctgcat ccataaaata tggtaccatg aaatacaggt
    24901 accatattta aatagctatt ttattttaat tactctatat ttagagtata aattaaattt
    24961 cataagtata ttacagtact aacttatttc agatttattc tccttgtacc cctcagtttt
    25021 agtaattctt ctataaagca tacatagtaa aattaaaatt cccatgtaac ctgtatatgg
    25081 atatagtgta cctactaact tatcaaatgg cagtaaacct cctatacaag ctaatatact
    25141 taccactatt gttataattt taaacttagg gtggtcatct tctacaaatc ggtttgttac
    25201 tgaccataac aatggtacag ctgtagtgta tattcctagt aaaagtacta ctgagaataa
    25261 aatacctatt attggagata tcttatcagc taagtaaagt gctggtatct cttttgtgta
    25321 tatatttcct atgtctgaaa gtaatgccaa attcatcatt atagctgctg ccataagtgc
    25381 tactccacct actataccac cccatacagc atctttctta tttactgcag aagctcccat
    25441 tcctgtcaag aatgttatta ctattattac attgtaacaa gtataaagta ctcccgagaa
    25501 atatccattt gatgtagcag ttttaatatt taaactatca accatatttc cagcatttga
    25561 taaagcatca atattgctaa ataaactaat tgctcctact aaaagtgtaa atattattat
    25621 tataggtcct atattaccta gtattttaga aagccttgtc aaacctaatg aaacagttat
    25681 tagggaaacg attgccatac caatcctacc tacatatgga tttaatccat agtactctga
    25741 taatgtagct cctgctcctg caatcattat tactaatgtt ccaaataaaa acaatggacc
    25801 aaaccattca aaaaatgttc caagatattt tccacaatat acttgataaa tttttattgg
    25861 ctccttaagt ttcaattctc ttcccttatc tataacttct gctccaaacc atgaaaatag
    25921 taccatagag attattccac ctataatacc cgcatatcca taaaacgtaa aaaattgcag
    25981 tatttcttga ccagttgcaa atccagaacc tattacagta gcaacataag ctccagcaaa
    26041 acttataacc gttttaatat ttactttgcc tgacatacac tactcccccc aattaaaatt
    26101 atttttaaag tgtaagttta ttaagattat attagactca ttattgtgat atttcaatat
    26161 atttttaatt atttaacatt agttaacaca ttcttaacca tcctttaaca tatatcccat
    26221 aatatgtata aatttagtag agtattatat aaaatgtatt gaccagatta aatactgttt
    26281 attcattata tgatgataaa ttaacattta ttaaaaatat ttaactcata ataaaaaagc
    26341 tgttagaaag taatcgtaaa attacattct aacagctttt tagttaaaaa ttattcacct
    26401 aaagcatctt ttcttaactg ctcaaccttg tcagttcttt cccaaggtaa atctatatca
    26461 gttctaccaa agtgaccata agcagcaacc tttttataaa gaggctttct taaatctaag
    26521 tctcttatta tcgctcccgg tcttaaatca aagtgtttat ttactaattc aacaagtttt
    26581 tcttctgaaa ccttgcctgt cccaaaagta tctataaata tagataatgg tctagctata
    26641 cctatagcat atgcaagttc tatctcacat ttgtctgcaa gaccagccgc aacaatattt
    26701 tttgcaacat atctagctgc gtaagcagca gatctatcaa cttttgtagg gtcttttcct
    26761 gagaaagctc ctccaccatg tctagaatat ccaccataag tatctattat tatttttctt
    26821 cctgtaagac ctgtatctcc ttgtggtcct cctataacga atcttcctgt tggatttata
    26881 taaattttag tctcttcatc aagtaattct gctggtataa cttccttaat aacatgatta
    26941 attaaatctt ctcttatttt atcatttgat actgtttcac aatgttgagc tgatataaga
    27001 actgtatgta ctcttacagc tttacttcct tcatattcaa cagtaacttg agtttttcca
    27061 tctggtctta aataatcaac taaacctgtt tttctaactt ctgtaagtct tcttgataat
    27121 ttatgtgcca aagatatcgg aagtggcatt aattctggag tctcattaca tgcaaatcca
    27181 aacattatac cttggtctcc agctccaact ttttctattt cttcttctat ctcttctcca
    27241 gtcttacttt ctagaccctc atcaactccc atagctatat ctccagattg ttcatcaata
    27301 gaagttataa ctgcgcaagt atcacaatca aatccatatt ttgctcttgt atatccaatt
    27361 tctcttactg tttctcttac taatttagga atgtctacat aagcagatgt acttatttct
    27421 cctgctacta aaactaaacc agtagtaact gtagtttcac aagctactct agattgtggg
    27481 tctttttcta ataatgcatc taatattgaa tctgatattt ggtcacatat cttatcagga
    27541 tgtccttctg taactgattc tgacgtaaat aaatgtcttg ccatttttat tcccccttat
    27601 ttattcttaa tttttattat aggttttacc ttgtttttaa ttttagaaat ttactcaaaa
    27661 taaaaaaacc tctcctttat aagaagaggt taataatcgt aattacatac taattaactg
    27721 acctcatctt tcaagacttt tgtcttgtag gaattagcac cttttcccat ttgggtggtt
    27781 gctgggcttc acagggccta tccctcagcc tctcttgata aggtaatcaa gatttaagtt
    27841 ttgttttact ttactagctt actatttttt taaaattttg tcaatatttt tagtcatatt
    27901 ttttctattc ctttttttgt aagacaaata aaattttcta tctatctgaa atttaaagtt
    27961 aggtcttttt aatgcttttt attcatatat ttttttgaag ggggtgttcc tattgtattt
    28021 agctaaagta aactacagcg atgatgatgt tatacagatg ctaagttcag ttctaaaaac
    28081 tataattaat gaaaatacga ttgttgtttg cataggaaca gacagggcta taggtgatac
    28141 tttaggccct ttagttggaa caatacttaa gaacagtaac tttaaatacc ccgtttacgg
    28201 tacactagat aaccctattc acgccttaaa tatatatgag tctttagata ctataaaaaa
    28261 tacacatata caaggtaact tcttggctat agatgcttgc ttggggtcgc agagtaacat
    28321 aggtaatata cagattagag aaggacctat cctgcccgga aaaggtgttg gtaaaaaact
    28381 acctcaaata ggaaattatt ctatagtagg aattgtagat aaaattgatg aaaataataa
    28441 actttcattt aataatatac gccttagctt tatattagat ttagctgaaa caatagcctt
    28501 ggctttatta gtatccacct aatctaaata taaactaaat gataaaattc tttatataaa
    28561 aaggtagata ttttagagta tctgattaaa tactccaaat tatctacctt tttactttta
    28621 tatttcaagt ttttattttt ctactaaagc aaacattaat tcacctttac agacaagttc
    28681 ttcacctaca tatgccttag catctgcctt agctatattt tttctaagag ttgtcatttc
    28741 aacttccata gttaatgtat ctcctggtct tacttctctc ttgattctaa ctttatctat
    28801 acctgcaaat acacctagtt ttcctttgtt ttcctctaca gacatcattg ccactccaca
    28861 tacttgtgct agagcttcta ctattagtac tcctggcatc aaaggatatt ctggaaagtg
    28921 accttggaaa aatggttcat taactgttat attctttata ccaacagctc ttttcccaac
    28981 ttctagctct gtaatcttgt ctactaataa aaatggatat ctatgtggta ttaatttttt
    29041 tatttcatct atatttaaca ttctaaactc tcctaattat tttcttttga aagacccacc
    29101 agtttcaagt aagtctattg aacctaaagc tattcctgta ccttttgcaa cacaagataa
    29161 tggttcatct gctattgtaa cagttattcc tgtcctctgt tctattattt tatctaatcc
    29221 atataataat gaacctcctc cagtcattat tataccgaca tctcctatat ctgctgctaa
    29281 ctcaggaggc gttttttcca aaacagcatg agtagcaact actatttgct caacacattc
    29341 tctaagtgct tccaacattt ctgttgaatt tatagttata gaactaggaa gtcctgtaat
    29401 taaatttctt cctgttatct tcatatattt ttcttcttct ctcttaaatg cagaacctat
    29461 ttcaaacttg attttctctg ctgttctttc tcctataaga agattgtgtt gttttttcat
    29521 atatttaact atagcatcat caaatctatc tccaccaacc tttatagatt cacttacaac
    29581 agctcctcct agagatatta cagctatatc tacagtacct ccaccaatat ctataatcat
    29641 attaccatta ggctgtgata tgtctacacc agctcctatg gcagctgcta ttggttcttc
    29701 tatgatatat acttctctag ctccagcttg tctcgtagca tcttctactg ctcttttttc
    29761 cacttctgtg acacctgttg gtacgcatac cataattctt ggcataaaaa atcttccaaa
    29821 tcccttttta tcaactatct tatctataaa agattttaac atcttctcag ttacatcata
    29881 atctgaaatt acaccatctc ttaaaggtct tatagcgact atattacctg gtgttctccc
    29941 tatcatcttt ttagcttctt ctcccacagc taaaacagaa tctgttcttt tgtctatcgc
    30001 cacaacagat ggttcttcta aaacgattcc cttaccacta acataaacaa gcacatttgc
    30061 agtacctaaa tctatgccta tatcagctcc agccattttt tcaactcctt tatttataat
    30121 attagattat attataagcc tattttaata ctcctataat aaagcatata caaaaatctc
    30181 gctttaatca agagaaaata aatcttttga gagtattatt attttgctat ttataattac
    30241 ttaatatatt tctttttata atttaattca ataaaaaaag taggttttta acctacattt
    30301 ttttatgttc cttctcatat tttaatttcg ttgctaatcc ccctctaata tgtctctctg
    30361 atttattttt gtccagcaca tttttaactt ctttggcaag ggatggattt atttccttca
    30421 atctttctgt aacatcctta tgaattgtac ttttacttac tccaaatgtc ttggcggttt
    30481 gtcttactgt agtgtttttt tcaagtatat attttgctac cactatggcc ctttcctcta
    30541 tatgagatct caaagattac ccccctcaca taatagcttt aatgatatat atgtaaaaac
    30601 accaaaaaat ataacaagct taaccaaata tgattaaact tgttatacta ttacttaatt
    30661 agactcattg ggtctataga tttttctcca ctatacgcct ctaaatgcac atgtatacct
    30721 tcttcacttt ctatttgaga agtactacct acagtaccta gagattgtcc ttctgttacc
    30781 ttctgttctt tcttaacact aacattttta tctagatttg aatatactac tacaatgtta
    30841 ttgtcacttt ttattttgac tgattgtcca tattcttcat catcaaatac atctactact
    30901 attccgttta atagagattt tacttctttg cctttagtac aactaatatc tacaccttta
    30961 tgagtttccc aaacatcaag tgttttagaa taactaggct ctttttctga atatcctctt
    31021 attactttat ctccaatgta atttaatttt gttgtactcg atttattttc ctcttttgac
    31081 ttagcttttt ctaagttttg ttttgaatcc gttgcagtag ggatagcatc tttcttgtct
    31141 ttttcaatta aatgtatttc ctcttcacta tctttattag cattttccat aattccttta
    31201 ttagaagcca atttatctac attattattt gtaaaccaaa ctccacctac tgctaataaa
    31261 caaacacata caaacaaaga taaataaaaa ccatcttttt ctaacagctt tttcttcatt
    31321 ttgcacctcc aaatatattt ctacatttgg attattgacg tgtttttggc attttataca
    31381 attattagta tatatctttt atttttgtat ctgtgtagta atgtgataat atatcataat
    31441 atttataacc ctcttctgcc attccttctg ctccccactg gctcatacca acaccatgac
    31501 catatccttt taccacgaaa tctatgtatc cttctccaaa ttttatgtca aagtttgcag
    31561 agttcaactt aaaaacagtt ctaatgtctt ttccagtcaa ttctttattt cctaatttta
    31621 ttttttctac agttccggca tcacttcttt tagttattga aacttgttta cttaaattat
    31681 tcacatctat cacaatagtg ctatatgccc ttctaagact tttaacaaaa tctgtgttac
    31741 ttattttaag tgttgacgca aatttagggg aatacttatc ataaggactt tccacagact
    31801 ttaaataagg atattttgca gaaaatactt cttcactatt ttctgttttt cctgaagatg
    31861 tagaaaaata aagaggaagt atcgccttat cattataagt tattatttga cctttagttc
    31921 ctctaactgc ttcttggatt ttggaatatt tattcttaat ccattcttca ccatttaatt
    31981 ttttaagagt atcataactc ttatattctt gacaatgttt ataatcagta catactactg
    32041 catttttatg tttactagat ttaccatgtt cctgtttata tactacatat gttctagctg
    32101 ctacagattg agcttttaaa gcttctatat caaactctga agacatctca ccagctagta
    32161 ctccacataa atagttctcc atgtccatct tttcggtttt ccctgttata tgattatata
    32221 cattgataat tggtgctttt ttatttacag tttcatagtt cactgtattt ttttcttctt
    32281 tattaccttt gtcttcaata tcactttttt taatagtttt gtttatagat actgggctct
    32341 ctggtttttc agttaattct acatttttat aagaaactaa agttataagt gatggaacta
    32401 acacagagca agtcacaaat cctaacaaaa caaccaatgg gttcttcata tactctcctc
    32461 cttagctttt aaaaccttgt atttaagtat atgaacaacc ctattaattt ataactttct
    32521 tctataaacc aattattaat cttctactat ttcgatttga ccacctagtg ctgttatctt
    32581 cttatctata tcaacataac ctctttggat atggtatatt tctcctattt gagtttctcc
    32641 ttctgctata agaccgcata aaataagggc tgcccctgct cttaaatcag tagcgtttac
    32701 tgctgcaccg tgtagctcat ctacaccatt aacaaccgca cttcttccat ctatttttat
    32761 attagcaccc attctattaa attcagcaac atgcataaat ctgttttcaa aaacagtttc
    32821 tataactaca ccagttccat tggctacagt aagcatagcc ataaattgtg cttgaacatc
    32881 tgtaggaaat cctggatgtg gtaaagtttt tatatctatt ggttttaata cttttggacc
    32941 tacaactctt acagaattgt ccatttctgt tatttcacaa ccagcttctc ttaatttagc
    33001 tacaactggt tttaaatgct ccattaaaac attttcaaca gttatatcac cttttgtcat
    33061 agctgctgca accatataag ttgctgcttc tattctgtct ggaattacat tatgttctgc
    33121 tccttttaac tctttaacac cttttatttt tatagtatta gtacctgcac ctttaacatc
    33181 tgctcccatt tcgttcaaga agtttgctaa atctactatt tctggctctt ctgctgcatt
    33241 ctctattatt gtagtccctt ctgctaaaga agctgccatc attatgtttt cagttgctcc
    33301 aacagatggg aaatctaaat ataatttatt tcctactaac ttttctgttg ctgcttcaac
    33361 aaaaccatgg tccatttcta tctttgcacc taaagcttta aatcctttta aatgtaaatc
    33421 tataggtctt gttcctatag cacatcctcc tggcatagaa attttcgtac tattaaatct
    33481 agcaagcaat ggtcccataa ccaaaaaaga agctctcatc tttcttacaa gctcatatgg
    33541 agcttcacaa gtttttatat tgcttgcatc aacagtaagt gtattttctt tatattcaac
    33601 ttctgctcct acatgtctta ataaatctga tataacatga acatctctta aatttggtac
    33661 tccatttaat gttgattttc cgtttgctaa taaagtcgct gctattattg gtaaaactgc
    33721 attttttgca ccatctattt taacactacc tttaagtgga ttactctttt ttactattat
    33781 cttagccata ttttttgatc tcctcataat taataatcta attttataat tggtgtggca
    33841 atatatatga gtgtttttcc gtcatcctca ttatatttaa ttcctatatt taaattaatt
    33901 ttttccccta aatattctaa ttcataatca tttaatactt tactatatgc tgttatagat
    33961 aaaaagttta cgtctttaac ttcatctatt tgcttagcac tcatattata taatattttt
    34021 tgtaaaatat catcatattt gttgttttgt aacctttttg tgtattctcc aacaatacaa
    34081 gcagacacat ctacttgaga tgaatattta tttaacacat tttctacgtt actataaata
    34141 acctctttat gtttatatac tttgttatcc aatatgtcaa ctactatgta actttctttg
    34201 ttatttttat ttattccttt tatagaaatt tctttatcgc taccttttat agtagcacat
    34261 aattgtattt tatcgccaga ccctttttga ttccaattta ttcctttttt atccaacttt
    34321 agattgttaa ttatttctat aaaaatatta tgaaattctt tatttgataa attatgattt
    34381 ataacagaat taattttaac attataaaat ttaaaatttg cttctgatat ttcaagagct
    34441 ttacttaatc tattgtatct agtatttata cttgtatctg cataagatat tgtcattcct
    34501 ataatcatta atataaaaaa gcttatagct aattttactt ttttcatagt aaaaaatccc
    34561 ccttaaatat gtttttctgt ataatagaat tattaacaaa tttaagagag atatactttc
    34621 tcaatatgta tttttataca tttttgtatt tttcatctaa taatctcttt caaactcttc
    34681 aatctcaatt ttctctatcc ttctcctgga cttgccaatt tcttatatgt atcaaataca
    34741 tcttgcatta attcttatat attctcttct gtaatataaa ttacttagtc tatacttaaa
    34801 tctataatct ataatctata atctataatc tataatctat tcatatttta ccaacaaaat
    34861 aaacagataa tactttttta tcaatttcca tagctccaaa tgaacatacc taagtacact
    34921 tcccaataca caatttacac actcttccta ataaatcaaa aacacctttg ttttaaacaa
    34981 ctctattgca ctttcaaaaa ctttatttgt gttctcccac ttcaattaaa tttgaattat
    35041 caagactcca gcccatccat ccatcactat aaagggaggt attttccact cctatgacat
    35101 ttgcatatgt taatacttca gcagcacgcc atccacttcc acacataaat ataagctgtt
    35161 tattcacatc tatattttcc ttgtcccaca tttctaaaat ctcatattta tttctcattg
    35221 tattatcaat attacgatat tcttctagtg aaacactatc actacctgaa tgtccatata
    35281 aagctcctgg tatacgccct tttttatcat aatatgtata tccagatact tttcctatat
    35341 gttcatccca agtacggtta tctactagta taaacttatt tttttctttt agtttttgtc
    35401 ttaattcaga tgtagtaaca attaattgtg agttaactgg tatatctgct ccaaaattag
    35461 tacatgaatg ttttggatta ctaataaatt ctaattcata tcctgctgat aaccatgagt
    35521 ttgttccacc attcaaaact cttacatctt ttactcctat atatctcaat ataacagcta
    35581 gtctataaga tgccataggt gtactacttg aaactattac agtatcatcc tttgttaatc
    35641 catagtctaa agcaaactta gttaagttat catcattatc aagcatccat gttggaggag
    35701 gttctataat atcagtattt acatgaacac ttgtaggtat atgccctttt gtatacgatt
    35761 cttctctcca actcgcttca atcattttta tattctttga atcttcaaat gtctcaggaa
    35821 tttttccatc taatatatct tttattataa aagcaggaac tatcatttga taatttttat
    35881 atctttccat tggaagattt tcgtcgtctg cccattgttt aatatcatat ttatatagat
    35941 atttatatcc ttttttagat aaataatctg ctacaaccaa agcatctttt ccatttgcat
    36001 catacaatac tatattttta tctaaatcaa ttcttttagt tttcaatgct tgttctaaca
    36061 cttcatcttt tctatctgag tatacactta accaattagc agaaaaatct actgctcctt
    36121 ttatatgtcc acctcttttt actccatcta acttccagcc attataagca tcatttaagc
    36181 gagtatcaac tataacccaa gcgttatctt gcactttttc ttgtaaaact tcagtagata
    36241 ttgccttaag attaacaaaa taatcttctt tcttttcttt tttaaaatca tttgtcataa
    36301 tttatactcc caatttaaaa aaatttttat ttgaaattat tttactaaaa taaagtaatt
    36361 ttcttatact ctattagctt cattatattc ttttataaat agttccttat attcttattt
    36421 taaactactc actttatctt atcacaaggt tactaatacc ttgtatttta atttttctca
    36481 taagctatta tttttttatc tgatattgac tatatgttta ttaagtactc actatatttt
    36541 atatgaacta tatttatcgt tccctatacc tggtatggtc tatatattta tcattttaca
    36601 gatgatttaa tatcaatctt tttataaaat ttattatagc aaaaacaaaa caaatttaat
    36661 atttatctaa aaaactcatc ctataaaata caaataacag gatgagttct tttcaataaa
    36721 tattatctat ttaattttta atctgttttt attcaactgt tactgattta gctagatttc
    36781 ttggcttatc tacatcgcat cctcttagta cagagacatg ataagcaagt aattgcattg
    36841 gtacaactgc taatattggt gataaaatat catctgaatt tggtatatag attattttat
    36901 cagcagcttt ctcaacatcc ttattatggc tttgagctat tgctactaca taagctcctc
    36961 ttgctcttac ttcttccata ttagaaacca ttttttcaaa taacttttct tgagtagcta
    37021 tggcaattac aggagttcct ttttctatta aagctattgt tccatgcttt agctctcctg
    37081 ctgcaaaagc ttctgcatgt atatatgaaa tctcttttaa tttcaatgaa ccttccattg
    37141 ctaagctata gtctatacct cttcctaaat agaatgcatg ttcagaacta acaactgttt
    37201 tagcaacttc ttttatatat tcttcattgt ctagtatttc ttgtattttt gatggtattt
    37261 ctttcatctt agaaatcata ctatcataaa attctctagt tattgttcct tttttgatag
    37321 caaagtctaa agctatcatg tataaagaag ttatttgagt agtatatgcc ttagttgatg
    37381 caactgctac ttcaggtcct gcccaagtat agaatacatc atctgattct cttgctatag
    37441 atgaacctac aacattagtt attgaaagta ttcttgctcc tttagccttt gaatctctta
    37501 atactgctag agtatctgct gtctcaccag attgactaac taatattact aatgaatttt
    37561 catctacaaa tgggtcgcta tatctaaatt ctgatgctat atcagttatt actggtatat
    37621 ttacaaattt ttctatggca tacttaccaa gtaatccagc attatatgct gttccacaag
    37681 ccactatata aactttattt attttttcta agtcttcttt agaaatattt atactgtcta
    37741 gaattatatt tccattatca tcaagtcttc tttctaaagt ttctcttact cctgttggtt
    37801 gctcatgaat ttcttttgac ataaagtagt catatccacc tttagacgca gcttctacat
    37861 cccaagttac atgaaatact tctttttcaa caagatttct attagagtca taaactgtta
    37921 cattttcgtc ttttagatga actacttctc cattttctaa gaaataaaca tctctagtat
    37981 attttaataa tgcaggtata tctgatgcta taaagttttc accttcacca acaccaacta
    38041 caagaggact atcttttctt actgcaacta attcatttcc atgttcttta catataacac
    38101 ctaatgcata agctcctctt aattttgata tagttttgta tacagcatct aataaattac
    38161 cctcatagta tttatctact aaatgtgcta ttacctctgt atctgtttga gattcaaact
    38221 taacaccttc agaaattaat tcttctttta tttccatata gttttctatt atcccattat
    38281 gaacaactgc tattgttttg gcttgattaa aatgaggatg tgagtttaca tcagatggtt
    38341 ctccatgagt tgcccatctc gtatgcccta tacctaaatt tccatcaata ggattctttt
    38401 ctaagtcttc tgcaagtaca gaaagtctac ctttaaactt tctaatatta agttcttttt
    38461 cattagaact attaactgct actcctgctg agtcataacc tctgtactct agcttagaaa
    38521 gcccttctac tataacttct gctgcctttc tacttcctaa atatccaact attccacaca
    38581 ttatttgtgt cctcctaaaa atattattta tatattttaa gagttaggtc atataagttt
    38641 actttggctt ccctttgtcc ataggattgc tcctattatt taaagaagtc tttcgatggt
    38701 aaacacaccg cagagcatcc gccgacaatt cgattaactc tgacctcgtc aacttagttc
    38761 ctatattact caataattat gtctataact aagttctggc gcttagaaag gtgtcctcag
    38821 tatttatatc tgagaatacc ctttaaatta cgataatttt tcttgtataa gattagctaa
    38881 gtttgttgct aattctttta tttgaccttc ctctttaccc tctaacataa ctctaactaa
    38941 tggctcagtt cctgatggtc ttatcaaaac tctaccattt ccgtctaaaa tagattctat
    39001 tctttctatc tcagttttta tttctggata ttccatatat ttgttcttat tttcattttt
    39061 tattgttgca tttactaata cttgtgggta ttggctcatt attgatgcaa tttctgataa
    39121 aggtttcttt tcttgcaaga ttatatttgc aagtattaga gaactaagca ctccatctcc
    39181 agtagtgttg taatctaaaa atatcatatg tccagattgc tctccaccaa gattatatcc
    39241 atttttaacc atatcttcta aaacatatct gtctccaact gctgtagtag ataaatttat
    39301 tccattttcc ttagcagcta ttgtcaaccc tatattactc ataactgtaa ctaccaaagt
    39361 atcttgtgcc aatttattat ttttctttaa atttaaggca ctcaatatca ttatatggtc
    39421 tccatcaaca atttgaccct tttcatttac tgcaattaat ctatcagcat ctccatcata
    39481 tgcaagacct aagtcagcat tatgttccaa aacagccctt tgaagttgtt ctggatgtgt
    39541 agagccacat ttatagttta tattatttcc atctggtgaa ctatttattg aaattacact
    39601 tgctcctagt tcatcaaaca ctattggtgc cactttatat gacgcaccat tagcacagtc
    39661 caaaactact tttaatccct taaagtcagt gcttattatt gattttaggt agtctacata
    39721 gtctctttgt gcacaatgtt catgtaattt tcttcctaca ttttcaccaa ttggaagaca
    39781 atcaattttg tctatatcgt ctatatattc ttctattttc aattcaattt catcatctaa
    39841 tttatatcca ttcttattaa agaatttgat tccattatat tcaacaggat tgtgagatgc
    39901 agatataacc acaccacaat cagcaccata ttttctagtt aaatatgcca ctgcaggtgt
    39961 tggtattatt ccaacagtaa taacatcaca tccaacagac ataagtcctg ctattaaaga
    40021 tgcttccaac atatcacctg atattctagt atccttacct actactactt ttactctgtg
    40081 gtctccttga gctaagacaa atccaccagc tctaccaagt ttatatgcta aatcacatgt
    40141 tagttctgta tttgctactc ctctaactcc atctgttcca aaatactttc tcactaaaga
    40201 ttctcctttc ataattgaaa agcttgtaat ttgtattata tttttatagt tcataaaata
    40261 tcattatagg cttaatttca gtcataaaaa tattttaata cttatcagca aaaaagacaa
    40321 tagaaataat tctattgtct tttttttgac atctttttct ttattagtta gttgctttag
    40381 aacatgtagc atccatacct ttttgtaaag cttctctgtt taatggaacg aatttttctt
    40441 ttctaggtcc gaaaactttt ttgaatgctt ctagtacaga ttctatatct acagtatcag
    40501 atacttttaa gtaagcgcct aacattatca tgttagcaac tttatcattt cctaattcag
    40561 ctgctaattc atttgctggt atataataaa catctacatc tgttctatca actttttctt
    40621 ttattaaaga actatttact attatttttc caccaggaat aacatcatct ttgaattttt
    40681 caaatgctgg tatatttaat aatatagctg cagttgcatc atctgttata agtggagaac
    40741 caactggctc attagataca gttactgcac agtttgctgt acctccacgc atttctggtc
    40801 cataagatgg taaccaagaa acttcttttc cttctagcat tcctgcataa gtaagtaatt
    40861 gtcccataga cataacacct tggcctccga agcctgcaca tattactcta gctgtagaca
    40921 tttacttcac ctcctcaact tcaacatttt tgaaatttcc taatggatag tatggtatca
    40981 tgttgtctct taaccattgt aaagcatcgt ttggagctag tccccagttt gtaggacaag
    41041 tagataatac ttctactatt ccaaatccta atcctgcttg ttgaacttgg aatgctttct
    41101 ttatagcttt tttagcttgt ctaacatgag ctggagtatc aactgctact ctttctacaa
    41161 atacagctcc tgttaatgta gctaacattt cacttactct tattggataa ccttgagttt
    41221 cagcatttct tcctgattga gcagtagtag ctctttgtcc aactaatgta gttggagcca
    41281 tttgtccacc agtcatacca taagttgtat tgtttacaaa tatagtagtt attttttctc
    41341 ctctagcagc tgcatgaact atttcagcag taccaattga agctaagtct ccatcacctt
    41401 gataagtgaa tactgtattt tcaggatgaa ctctttttat tcctgtagca gcagctggcg
    41461 ctctaccatg agcagcttct tgagtgtcac agttaaaata tttatatcct aaaactgaac
    41521 atccaactgg aacaacacct acagcatcac ctaatactcc tagttcttct aatacttcac
    41581 caactaatct atggattata ccatgtgtac atcctggaca gtagtgagtt tgagtatctt
    41641 gtaatccttc agttttctta aatacaacag ccattatcta gcacctcctg caaccaattc
    41701 tccggcaata atttttttag ctttttcaac tatttctgct ggtgttggag tcattcctcc
    41761 tggtcttcca tggaagtaaa ctggtaattt accttctact gccattttaa catcttccac
    41821 catttgtccc atactcattt caacagtcaa tatgtttcta gcattaggaa tttggttgaa
    41881 tgcttcgaat gggaatggcc ataatacttt aggtcttata agacctgctt ttattccttc
    41941 ttctcttaat atatcaattg cgctttttac tactcttgaa gtagttccat aagctgcaaa
    42001 tacaaattca gcatcttctg ttttgtacat ttcgtattgt acttcattct tttccattgc
    42061 atcaaatttt tcttgtaaat gccaacaatg gtcttctagt acttctggct ctaagtatag
    42121 agagtttatt acattaggtt ttctttttcc tttagttcca acagttgccc aatctttagg
    42181 aggcaattct cttttcttct caggagctct aaattcaact ggctccatca tttgacctat
    42241 cataccatct gcaactacca taactggtga tctgtaataa tctgcaacgt caaaagcttc
    42301 cattatcata tcaacagctt cttgaactgt agctggtgcg aatactggag ttctgtagtc
    42361 tccatttcct ccacctcttg tagacataaa gtagtcagct tgtgaaggtt gtatacttcc
    42421 aagtcctgga ccacctctca ttacattaag tacaacacaa ggaacttctg ctcctactgc
    42481 atatgttata ccttcttgct ttaaagcaac tcctggtgaa gatgaagaag tcataactct
    42541 tgctcctgca cctgctccac cataaaccat gtttatcgca gaaacttctg attcagcttg
    42601 aacgaacgct ccacctattt taggtaactc tcttgataaa tattctggta attcactttg
    42661 tggagttatt gggtaaccga agaaatattt gcaaccagct tctatagctg cttttccaaa
    42721 tgcttcgtta cctttcataa gtatcttagc cattaaaatt cccccttaaa tattaattaa
    42781 attaatctct ttctactgtt ataactacat ctggacacat agttgcacaa cttgcacatc
    42841 ctatgcattt ttccatttca aaaacagttg ctgggttata cccttttttg tttattacat
    42901 ttgagtctaa ttgtatgatt tttactggac atgcttcaac acacaatcca caacctttac
    42961 aacgttcttg attaaaagat acttttcctt tagccattta cctaagcctc cttaaacttt
    43021 ctatatattt gagctcaaac tacatccagt cttctctcat atataatttt ataggaaaaa
    43081 tttctccttc taaatctttt ggtaactgtt caacaagatt ttctaaacat gcaacatatc
    43141 ttatcggtat attacatttt tcagaaactt cttttgcaac tttctgacct cttaaaacat
    43201 catcaatagt agtaaaccta ataagatgag tattattaat tagacctgtt acctttaatt
    43261 tagaagattt ttctatttct tttatatatt ggataacttc ttcagaagtt tgagttttct
    43321 ccctatttgc atttattaca aaaagcatat cataatcgcc ttcttttaac aaatgactaa
    43381 atcttcctat aaccctagca cctacattat ctccacctaa atctataaca gaattgtatg
    43441 aatggtctac cataggagac attacctctg ctgatactgc tggtaaatct aatgttggag
    43501 cattaattga gctatctatt ggttgtatac ctaaggattt cattaactct ttcttttctc
    43561 tagttctaaa gtatacattt accacatcta aatcggctat agcaactttg ccagaaacca
    43621 tatctcttaa cttaacaaca tagttcatcg caaattcact tttaccactt ccatagtgac
    43681 cagttatgat tcttattctt ttatcattag ttatcataaa cttgagcctc ttcttcacca
    43741 gttaaaactc taagtccacc ttgagctaat gcaatcattt catcttcacc tggataaact
    43801 tttacatctg ctataaattt aactctatct gcaatcattt ctgtaaacat ttttgaatat
    43861 gcgattccac cagttaataa tattgctttt acatctccct taagaactgc agcactagct
    43921 cctatttctt tagagatttg atatgccata gcttcatatg ctaatttagc tttttcatca
    43981 ccagcttcaa ttctttcttc aacttctcta gcatcattag tgtttaagta tgcaactaat
    44041 ccgccattac cttttatttt ctttttaatt tcatcttgag tatattttcc actaaagcac
    44101 atttttacta atgcacctac tggtagtcca ccacttcttt ctggagagaa aggtccttct
    44161 ccatctaatg cgtttgcaac atctactatt ttaccatttt tatgagctcc aacagaaact
    44221 cctccaccca tatgtgcaac tataagattt atatcttcat atttcttgtt tatttctcta
    44281 gcatatcttc ttgctattgc cttttgattt aaagcatgta ctacacttgc tctacttatt
    44341 tcaggcatac cagaaattct agcaacatct tctaattcat ctacaacaac agggtctact
    44401 atgtatgaag gaacatttac ttcttcacct atttgttttg ctattattcc acctaggttt
    44461 gaagcgtgtt ctcctaaaac tcccactttt aaatcttcaa tcatagcagc acttactgaa
    44521 taagtaccac cttttatagg tttaagaagt cctcctctac ctactacagc atctaattca
    44581 gatgttttta ctccaccttc ttttagagct tcttctatta cttgtttacg aaattcaaat
    44641 tggtcagaca ccttctcata ttttcctatt tcttctgaag aatgtcttaa agttttttca
    44701 aatactaaat cctcattatc aaatacagct attttagttg atgtcgaacc aggatttatt
    44761 gttaagattt taaatatttt gctcatttgc ctatttcccc cttaaaatta tgcctttgct
    44821 gccattaaaa cacctaaagc tattgagttt agtttagttt cttcactgtc tgctctagaa
    44881 gttaatatta ttggtgcttt agccccaact ataactcctg cattttttga ttttgagaag
    44941 aataccaaag ctttatataa tatgttacca ccttcaatat ctggggctaa taatatatca
    45001 gctcttcctg ctacaggatg atttatacct ttatgtttag ctgcttctaa agatactgca
    45061 ttatcaattg caaaaggtcc accaaccata caacctttga tttctcctct ttcatacatt
    45121 tcttctagtt ctttagcttc aactgtatct ttcatttttg gatttacttt ttcttttgcg
    45181 catattgcag caacttttgg ttcacttata tctaatgaat gtgctactgt gcaagcattt
    45241 tctatgattt gctttttagt atttgtatca ggagctaagt tcatagctgc gtcagttacg
    45301 aaaaataatc tatcatatcc ctctacatca aatactgcta cgtgacttaa tacatttcca
    45361 gttctaagac ctacttcttt atttaaaact gcttttagta ttattgatgt gtctactaag
    45421 cctttcatta ccatgtcggc ttttccttgt gaaactaatt caacagattt tagagatgct
    45481 tctgctaaat cttttatatc tatcagttca taattttcga tatccatgtc tatgcttttt
    45541 gcaatttctt tagtcttttc tatatctcct actaaaatgg catttgctat tttttctttt
    45601 ctagccattt caactgccat taaaacttct ttatcttggc aacatgctac tgatatagtt
    45661 ttaggtcctc tttcttttgc aaacttaatt acttcttcaa aacttctcat gcatttccac
    45721 ctcatcttga attttttgaa atagaacttt ttatatctca attaaattat aactcgttca
    45781 gcaacgtttt cctagagata attgaaatat ttaattcaaa atatttattt aaatctattt
    45841 atcagtcttt tttcatatat ctagcgcttt ttagatttaa caaatatctc attgtcctta
    45901 ttttataata taactaaaat aaacaagatg caacaaaaac ataaaattaa caaacttaat
    45961 tttataggtt tttttgtcgc atcttgtaac aactaaatat tcgaaatatt tgttcattcc
    46021 acttcaacaa tattaggttc tatatttatt tttttaaatt gatatgttgt ttcatatttt
    46081 attttatatt tactatcttc ctcaggacct tgtgataaat caatatataa tgaaatatct
    46141 tttttattta acttactcaa atcctccatt gactctacaa caacttttat atctacatca
    46201 gaaattacat ttggattatc catatttgtt gggatatttc ttaattcaat atctcccgca
    46261 ttatataaaa attctgcact caattgtttt gtatcattta atttaattgt tatagagcta
    46321 tcctcaagag ttataccctc tggaatttct aagctaactt ctttttcttc accattagcc
    46381 aaatcagcta agtcaatagg ttttgtctta atataactta ttttgtctat atcttctttc
    46441 tttcctttta ttgtaacttg ttccttactt aatttatagt ttttcagtac atcattatct
    46501 ttattatcat ttagacttac tcttattggg acaatttttt ccttcaatag ttctatcttt
    46561 gcagtcacaa aggatgaact taatgttact ccttcaacct tagttccatc actatttaat
    46621 ggtattaatt gcaatttttt agaaaaatca tctgtctcat taccaacatc tagattagct
    46681 cttacacttt tgacttcttt aactagagtt cttggacctg atacatcgac aaattcctta
    46741 ctaagttcaa ttttatttat atttcttttt gaatttccac ttacttctac ctttatatct
    46801 cttttttctt ttatgttttt ttcaagattt actactaaaa catcatgctt aaattctaga
    46861 gtaacacctt gaggaatatt tgcttttaaa tatacttcct tctgtccttg tattgggtct
    46921 tcaatggttc catatacatg tatatcttct tttttaacac tttgtacctt tgataaacta
    46981 ccttttatat atacatctgc tgttaatttt tcgtctggat atataacaag atttttatcc
    47041 tttaaatcac tcatattact tatagataca ggtatatctt caaacccttt agtttcttca
    47101 gggtcaacta cagccataac atacatccat aatgcaattg cacttaaaaa tgatattatt
    47161 tttattttgg tattattttt tagtctattt atcatttaaa tataccacct ttgaaaaaac
    47221 ttcttgtttc tgtatttgtt tttaaattac tacgtaatat attcatcatt ctttctctag
    47281 aaatatctct atataactta cctgctttag caattgatac gccacctgtt tcctctgata
    47341 caatcaaagt tatacagtca gacacctcac ttacccctat tcctgctcta tgtctagtac
    47401 ccaaatcttt acttaaatcc ttactttctg tcaatggcaa aaaacatgca gctgctttta
    47461 ccttagaatc tctaattaca acagctccat cgtgtaatgg tgtatttggt ataaatatat
    47521 ttattaatag ttgcctagaa atttcagcat caataatagt tcctgtattt attatatcac
    47581 ttattctagt ttctctctcc attatgataa gagctcctat tttttgtcta gaaagtgaat
    47641 ataaagcttc tacaatttct tctatatttc tattgagagt ttcttcagaa gtatttacat
    47701 tctttccaaa taaattaaac tttgctcttc ctatatgttc aagtcctgct ctaaattctg
    47761 gttgaaaaat tataagagca gctataactc cataatctaa agctttaaga gatatccaat
    47821 aaacggtatg caatttaaag gtgttactaa gttgagttgc taataaaaga aatattatac
    47881 ctttaaacac ttgttctgct cttgtatctt ttataaacat aaatattttg taaaaaatat
    47941 acgctactat agatatgtca attaaatcat atatactcat tcttaatatt atattaaaaa
    48001 atccatttaa aaaagaattt atatctaaca cagttttatc ctcctgtttg ataacttctt
    48061 aattatttac actaagtatt atactatata aatctaactc tatacaaact atttaacttt
    48121 tatgtaaata attctagttt tatatttgga gattgttaaa taatctctaa acacattaaa
    48181 aatatcaact taataaatac atatttctta ttaaagttag tttttactca aataactcaa
    48241 accatcaagg cgcgaataca aaatagtact cgcgccttaa ttcagtttta tgttctaaaa
    48301 tatcttaata tatctttgtt taatatatat taattattat aaattctata atccacttat
    48361 taactataaa caaatacaaa atctaaatat tacttatcat caatattatt ccattagatt
    48421 catttttata ttaaagtaat aacttatttc atatctagat tagataaatt tattttaata
    48481 tcttactata aactatcatt aaaacttatt atgtataact agatataatc tcctacagtg
    48541 aaaataatac acaataccta aagttatata tccatttcac agtataataa tttatttagt
    48601 ataacttata aatattacat taaattgtaa attcactata gtctctaatt ttttataatt
    48661 tcttatactt attgtttaga caaaaatatg ctcatgaatc tctttataaa taatttcata
    48721 agttatttca atatataaat accataaagt atacaatata atgtatataa ctcttactaa
    48781 ttagttatat ttattatatt taactgatgc cttcataaac atcttgtaga ctattataca
    48841 ttaccctccg tatgtttaca atattaatga atatactgta actctattag aatatactgt
    48901 aatttttaca tagtttaaag tttataaata aaaagacaca ggtaatatac atgtttcctg
    48961 tgtcttttta aacaatataa aagtttacta taatttatag taaacttttc ttaatgagcg
    49021 cacagggatt cgaaccccgg acacacgcct tagaagggcg ttgctctatc cagctgagct
    49081 atgcacccaa gttattatgg agcgggaaac gagattcgaa ctcgcgactt caaccttggc
    49141 aaggttgcgc tctaccactg aactattccc gcttatttaa tttggcgacc tggaaggggc
    49201 tcgaaccctc gacctccagc gtgacaggct ggcattctaa ccaactgaac taccaggccg
    49261 cgaatggtgg gaccaacagg gctcgaacct gtgaccccct gcttgtaagg caggtgctct
    49321 cccagctgag ctatggtccc atttttggag cgggtgaagg ggatcgaacc ctcacagctg
    49381 gcttggaagg ccagaactct accattgagc tacacccgca tatttaattg aattggtgac
    49441 tcgtagggga attgaacccc tgttaccgcc gtgaaagggc ggtgtcttaa cctcttgacc
    49501 aacgagccgt tttaatggtt gcggaggcag gacttgaacc tacgaccttc gggttatgag
    49561 cccgacgagc tgccaactgc tccaccccgc gatattgttt tataagtgcc gaagaccgga
    49621 atcgaaccgg tacgagaggt aagtcccgca ggattttaag tcctgtgcgt ctgccagttc
    49681 cgccacttcg gcttttttgg ctccaagggt ggggctcgaa cccacagcct accggttaac
    49741 agccggttgc tccaccgttg agctaccttg gaacattt
//
LOCUS       DAYAOY010000019        49209 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYAOY010000019 DAYAOY010000000
VERSION     DAYAOY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.019"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..672
                     /locus_tag="ACGI6B_001171"
     CDS             <1..672
                     /locus_tag="ACGI6B_001171"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFG9750626.1"
                     /translation="FMENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKS
                     RNGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKK
                     NGFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNND
                     IYKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNN
                     NKLKV"
     gene            831..1337
                     /locus_tag="ACGI6B_001172"
     CDS             831..1337
                     /locus_tag="ACGI6B_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFG9750627.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1465..2481
                     /locus_tag="ACGI6B_001173"
     CDS             1465..2481
                     /locus_tag="ACGI6B_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFG9750628.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     regulatory      2677..2771
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            2835..3539
                     /locus_tag="ACGI6B_001174"
     CDS             2835..3539
                     /locus_tag="ACGI6B_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFG9750629.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3834..4313
                     /locus_tag="ACGI6B_001175"
     CDS             3834..4313
                     /locus_tag="ACGI6B_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFG9750630.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
     gene            4951..5328
                     /locus_tag="ACGI6B_001176"
     CDS             4951..5328
                     /locus_tag="ACGI6B_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9750631.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            5321..6469
                     /locus_tag="ACGI6B_001177"
     CDS             5321..6469
                     /locus_tag="ACGI6B_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9750632.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            6486..7088
                     /locus_tag="ACGI6B_001178"
     CDS             6486..7088
                     /locus_tag="ACGI6B_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFG9750633.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(7181..8203)
                     /locus_tag="ACGI6B_001179"
     CDS             complement(7181..8203)
                     /locus_tag="ACGI6B_001179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFG9750634.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(8532..8714)
                     /locus_tag="ACGI6B_001180"
     CDS             complement(8532..8714)
                     /locus_tag="ACGI6B_001180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750635.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            9027..9446
                     /locus_tag="ACGI6B_001181"
     CDS             9027..9446
                     /locus_tag="ACGI6B_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HFG9750636.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            9577..11418
                     /locus_tag="ACGI6B_001182"
     CDS             9577..11418
                     /locus_tag="ACGI6B_001182"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9750637.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            11502..13332
                     /locus_tag="ACGI6B_001183"
                     /pseudo
     CDS             11502..13332
                     /locus_tag="ACGI6B_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            13340..15340
                     /locus_tag="ACGI6B_001184"
     CDS             13340..15340
                     /locus_tag="ACGI6B_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750638.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            15602..16861
                     /locus_tag="ACGI6B_001185"
     CDS             15602..16861
                     /locus_tag="ACGI6B_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9750639.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            16922..18709
                     /gene="iorA"
                     /locus_tag="ACGI6B_001186"
     CDS             16922..18709
                     /gene="iorA"
                     /locus_tag="ACGI6B_001186"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HFG9750640.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            18711..19286
                     /locus_tag="ACGI6B_001187"
     CDS             18711..19286
                     /locus_tag="ACGI6B_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HFG9750641.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            19339..20409
                     /gene="buk"
                     /locus_tag="ACGI6B_001188"
     CDS             19339..20409
                     /gene="buk"
                     /locus_tag="ACGI6B_001188"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9750642.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            20627..21328
                     /locus_tag="ACGI6B_001189"
     CDS             20627..21328
                     /locus_tag="ACGI6B_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9750643.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            21388..21894
                     /locus_tag="ACGI6B_001190"
     CDS             21388..21894
                     /locus_tag="ACGI6B_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFG9750644.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            22069..23091
                     /locus_tag="ACGI6B_001191"
     CDS             22069..23091
                     /locus_tag="ACGI6B_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9750645.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(23238..23546)
                     /locus_tag="ACGI6B_001192"
     CDS             complement(23238..23546)
                     /locus_tag="ACGI6B_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFG9750646.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(23606..24034)
                     /locus_tag="ACGI6B_001193"
     CDS             complement(23606..24034)
                     /locus_tag="ACGI6B_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750647.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(24257..25714)
                     /locus_tag="ACGI6B_001194"
     CDS             complement(24257..25714)
                     /locus_tag="ACGI6B_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFG9750648.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            26132..27046
                     /gene="nadA"
                     /locus_tag="ACGI6B_001195"
     CDS             26132..27046
                     /gene="nadA"
                     /locus_tag="ACGI6B_001195"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFG9750649.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            27059..28360
                     /locus_tag="ACGI6B_001196"
     CDS             27059..28360
                     /locus_tag="ACGI6B_001196"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFG9750650.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            28341..29177
                     /gene="nadC"
                     /locus_tag="ACGI6B_001197"
     CDS             28341..29177
                     /gene="nadC"
                     /locus_tag="ACGI6B_001197"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFG9750651.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            29433..29765
                     /gene="saoD"
                     /locus_tag="ACGI6B_001198"
     CDS             29433..29765
                     /gene="saoD"
                     /locus_tag="ACGI6B_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HFG9750652.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            29855..30112
                     /gene="saoT"
                     /locus_tag="ACGI6B_001199"
     CDS             29855..30112
                     /gene="saoT"
                     /locus_tag="ACGI6B_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:29888..29890,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HFG9750653.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            30139..31224
                     /gene="saoE"
                     /locus_tag="ACGI6B_001200"
     CDS             30139..31224
                     /gene="saoE"
                     /locus_tag="ACGI6B_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HFG9750654.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            31217..31675
                     /gene="saoC"
                     /locus_tag="ACGI6B_001201"
     CDS             31217..31675
                     /gene="saoC"
                     /locus_tag="ACGI6B_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HFG9750655.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            31710..32801
                     /gene="saoX"
                     /locus_tag="ACGI6B_001202"
     CDS             31710..32801
                     /gene="saoX"
                     /locus_tag="ACGI6B_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HFG9750656.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            32815..33603
                     /gene="saoB"
                     /locus_tag="ACGI6B_001203"
     CDS             32815..33603
                     /gene="saoB"
                     /locus_tag="ACGI6B_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:33028..33030,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HFG9750657.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKVLKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            33627..34370
                     /gene="saoP"
                     /locus_tag="ACGI6B_001204"
     CDS             33627..34370
                     /gene="saoP"
                     /locus_tag="ACGI6B_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:34191..34193,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HFG9750658.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            34370..35122
                     /gene="saoA"
                     /locus_tag="ACGI6B_001205"
     CDS             34370..35122
                     /gene="saoA"
                     /locus_tag="ACGI6B_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HFG9750659.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            35135..35776
                     /gene="saoL"
                     /locus_tag="ACGI6B_001206"
     CDS             35135..35776
                     /gene="saoL"
                     /locus_tag="ACGI6B_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:35618..35620,aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HFG9750660.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(35912..36733)
                     /gene="yidA"
                     /locus_tag="ACGI6B_001207"
     CDS             complement(35912..36733)
                     /gene="yidA"
                     /locus_tag="ACGI6B_001207"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFG9750661.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            37226..38920
                     /locus_tag="ACGI6B_001208"
     CDS             37226..38920
                     /locus_tag="ACGI6B_001208"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFG9750662.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      39252..39362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            39466..39837
                     /locus_tag="ACGI6B_001209"
     CDS             39466..39837
                     /locus_tag="ACGI6B_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HFG9750663.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            39932..40879
                     /gene="trxB"
                     /locus_tag="ACGI6B_001210"
     CDS             39932..40879
                     /gene="trxB"
                     /locus_tag="ACGI6B_001210"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFG9750664.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            40983..41300
                     /locus_tag="ACGI6B_001211"
     CDS             40983..41300
                     /locus_tag="ACGI6B_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFG9750665.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            41569..42855
                     /locus_tag="ACGI6B_001212"
     CDS             41569..42855
                     /locus_tag="ACGI6B_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9750666.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            42967..43440
                     /gene="grdA"
                     /locus_tag="ACGI6B_001213"
     CDS             42967..43440
                     /gene="grdA"
                     /locus_tag="ACGI6B_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:43099..43101,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HFG9750667.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            43472..44782
                     /gene="grdB"
                     /locus_tag="ACGI6B_001214"
     CDS             43472..44782
                     /gene="grdB"
                     /locus_tag="ACGI6B_001214"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:44519..44521,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HFG9750668.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            45038..46570
                     /gene="grdC"
                     /locus_tag="ACGI6B_001215"
     CDS             45038..46570
                     /gene="grdC"
                     /locus_tag="ACGI6B_001215"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HFG9750669.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            46581..47726
                     /gene="grdD"
                     /locus_tag="ACGI6B_001216"
     CDS             46581..47726
                     /gene="grdD"
                     /locus_tag="ACGI6B_001216"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HFG9750670.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            48036..49115
                     /locus_tag="ACGI6B_001217"
     CDS             48036..49115
                     /locus_tag="ACGI6B_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9750671.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
BASE COUNT        19529 a         5149 c         8728 g        15803 t
ORIGIN      
        1 tttatggaaa atataactag ttattttaca acaatactat gtgtatttat atgcttatca
       61 tctgtattta tatttactca actagctaga gtatttatta ataagaagaa gattaatcaa
      121 aaaattaaat caagaaacgg atttaggtat gatagagatt ttattgaagc cagaagagaa
      181 gaaatacata taaaggataa caataataat aaaaataaga gtaacaataa gaaattaaag
      241 ggagaaaaag tatttaaata cgataatggt gatttatata aaggtgagtt tgttgatgga
      301 aagaaaaatg gatttgggat atatattttt tcaagtaagg aaaagtatga aggattatgg
      361 aaagatgaca agatgcatgg aataggaaaa tatacttata gagatggttc aatatataca
      421 ggtgaattta aatacggatt aaaaaatggt ttgggaaaat taacatatcc caataatgac
      481 atttataagg gatatttttt agataataag aaaaatggta agggtgtatt atataaaaat
      541 gatggaaata aacaagcagg aatatgggaa gatgatgaac aatgtaaaag tttagatttt
      601 aaagatttaa ataataataa aataaataat gtttatcaaa atagattaaa taacaataaa
      661 ttaaaagttt aattattcta ataaaaaaat ctttatttat gatggaatat aggatatatc
      721 tatgttgtaa tttgtaaaat cttcctatat catataaaat taagtattga ttagtctatt
      781 acataaatat agaatataaa tatatccatt taataataag gagaagagat atgaaagagt
      841 atgtagttgt ggatttagaa actacaggat tagatccata taaaggttgt gaaataatag
      901 aaataggaat aacagaaata aaaaatgaac aaattgtaaa aaactattca aggttgataa
      961 aacctaaggg gataatttct tcatttataa cagaattgac acatataagt aatgaaatgg
     1021 tagaaaatga agaaccacta gaattagttt tacctagatt tagaaaatac ataggtgata
     1081 gaactataat tgcacacaat gcgaaatttg atttaaaatt tttaaattac tatcttagaa
     1141 tgttagattt agaaccaata aacaattata tatgtacagt agagttatta aaaaaatgta
     1201 aaagttataa aggaaaaaac aagaaacttg aaacagcttg tgcttattac aacatagaga
     1261 atataaatgc tcatagagct gacagtgata cattagcaac tgcaaaacta tttttgaaaa
     1321 taaaagatga atattaatta taaatataga gaaaattagt gaaaatatcc tttataaaat
     1381 tgacccttaa gcttacaatt ttgttctttg atttattttg tagaacaatg tataatgtac
     1441 cttatatgaa gaagggagac taaaatgggt tattacaatg ataatgataa taataaaata
     1501 agacgtaaaa aaattagtaa cagcaaagat agtaataaaa aatctaaaca ttcttatact
     1561 ccggcaaata aaaaaaataa aagttctaag acaagttcta gtaaaagaat gagtgaaaaa
     1621 gtagattcac agaatcttat aaaaagagat agagatagtt ttacaagaga taatgtaaga
     1681 cctttaaata cagattacaa tcaaagagaa agaagaagag tcgagtataa acaaagagag
     1741 aaaaaattaa aaagtaaaaa aagaaaaaaa caaaaaagga ttagaatagt tagagcatta
     1801 ttatctatta tgttagttgc actaattata ttttcagtgg caggtgcaat atttacagtt
     1861 tcagctataa aagggtcacc agaagttact aaaaagttaa ttaggtctaa atatataagt
     1921 agtgaggttg taagtataaa acaaatgcca aatgatttaa aacatgcaat agtttccata
     1981 gaagatgaga gattttataa gcataaagga gtagatgtta tttctctagt taggtcagta
     2041 ttaaacaatg tgtttacaga tacaacacaa ggtgcaagta ctatagatat gcaagtttca
     2101 aaaaacctgc ttactagtaa tgataaaact atgaagcgga aaataaggga tatgtataat
     2161 gctaagcaga tgaataagat tatgactaag gatgaaattc ttgaagcata tttaaataat
     2221 atgtatctag gtaaaaatgc atatggagct gcaaagggag ctgaagtcta ctttggtaaa
     2281 aaagttagtg aattaaattt agctgagtgt gctatgttag ctggtatgac aaataatcct
     2341 gcaaggtata ttgaccatgg agaggctaag aagagacaag tgattgtact gtataagatg
     2401 catcagcttg gttatataac tgataaggag tatagaatag caaaagctga tgacactcca
     2461 tttaaatcag aaattgatta aaattgtaag aatatagtat tttcaatagt ttaaacaaac
     2521 aaaaatgtaa taaaaaaggt gatttgccac ccatttgcca ccataattat aatctaatgg
     2581 caaattgttt taatgtacaa actagataat agaatattgt cttaaatgct atattatgct
     2641 aaaatttaat tgtattttgt aaattaaaaa acttataatt tagttttcta gggttccgca
     2701 ttttataatg gtcaggtcca agagaaaacg cgcaattgtt gtgtacacgg aaggataaaa
     2761 gcctgggaga tatttaagaa tatttctata ggctttttta tttttacata ttttaaattt
     2821 gaggaggttt tctaatgaaa aaatttgtta ttgaggatgc attttggaat ctatttccta
     2881 gtgcaaaaat tggagttgtt atttgtaatg atatagataa ctctataaaa gatgaagact
     2941 ataagaaaat aattttgaag ggagaagaag aagcattaaa atacttggag gactcagaat
     3001 ttagcaacaa ccaagtgatt aaagtatgga gagaagcatt taaaaaattt aaaacgaaaa
     3061 aaggagcaag gtcttctatt gaagcattat taaaaagagt tcataatgga aataatatag
     3121 gaactattaa ccctttagtg gatatctaca attttatctc attaaaatat gcattacctt
     3181 gtggagggga ggatatagat aaatttatag gagatattag gctaactaga gcaattggta
     3241 atgaggagtt tatcccacta gggacagatg aaaattcatt tccatatgaa ggagaaataa
     3301 tttataaaga tgatggtgga gcaatttgta gatgttggaa ttggcgtgaa gcagtgagaa
     3361 caatgcttac tgaagatact aacaatgcct ttttatgcat tgaattagta gatgaaagta
     3421 gatttaaaga atttgaaaat gctttaaatg aattagctaa aatagttcaa gaaaaattgg
     3481 gtgggaattg taaaatttca attttagata ttgataataa agaggtatca gtggattaat
     3541 atggaggtgc tgttgtaatg gactattcta gttataaaga tacttatatt tagggtaaga
     3601 aggtctattt tagatataac gattatcaaa ggatataata tagaaaataa aaatgtagaa
     3661 tataaaaagc ctttaaaata agaaaaaaat gtattttaaa ggcttttatt atactctttt
     3721 ttatttgcat aattaacaat aaaaatatat tgttaattat aatattttta ttgaaaaacg
     3781 atataatata atgtattata ctatatataa aatattttaa aggagttggc atgatgtcta
     3841 ataaacttat aaaaaattta ctaagtggta tattacaaat tttatttttt cttttaggat
     3901 tagtaattgt tgcaggagga tttaaaagtt ttatgtacct ctgttttagt ggagaagcaa
     3961 ctctacaagg tactatttat ggaatattaa tgtttatttt aggtgtatca tattttatca
     4021 ttataaaatc attgatagaa gtattaggta gttctgaata tagcctattt gtaaaagaga
     4081 atgtaaaaag atttaggatt attggatatc ttttgctttt aaactctcta attgagttta
     4141 tttcaacttt tggtactact ggaaaaggaa tgagatttct agatttagga tttggatttt
     4201 attttactgt tccagtattt gtatatttta taacttctct tatgagtttt gtaattgcag
     4261 atggatttgt aaaggcaata aagataaaag aggataatga tttaactatt tagatgtgac
     4321 actttaatat ataggagata aataacccga aaagagtgac taactagtaa tttaaaataa
     4381 aatatattag ggtataatac ataaactata atgctaaaat taaaagatta taatactaaa
     4441 atataattat gttttaatta aaataaagaa ctttattagt agatattaaa agcacatttt
     4501 tttataaaag tactttttta taatttatga aaggtataat tataaaagtc ttattcaata
     4561 aaatttataa ttaatttaga tatatttgta aataatcagt ataaaataga tgttttaata
     4621 gcataaaggc ataattagaa tgttattaaa atttattaga tattactcta gtttatttta
     4681 aaaaaaagta ttataatatt ccttgtgtga aaatatagga atttttatta aatagtttta
     4741 atgtgtaaat aacattctac actattgtaa ttaaatatgt aaaaagtaaa atatttaaat
     4801 agatatattt taaaatctaa cttttaatat tgtaaaaaaa ttagaaattt aacaaaaagt
     4861 acaaaaattc acattttttt cataaagtgt ttgactaaaa aattcacatg ttgtagaatt
     4921 aaggtagcag gagttttgag ggaggtgtaa ataatgataa gaaaaatacc acaagcagaa
     4981 ctaaaagtaa tgaaattcat ttggaaagta gatgttacag tgacatcaaa agatgttatt
     5041 gaagctatgg aacaaaaata tggctggaaa caaaccacca cactgacact tttatctagg
     5101 ctggtaaaaa gaggattctt agatgctcaa aaaatagaca gatatacaca ttacacaata
     5161 agagtaaaac acaaagatta tttaagtttt gagacaaagg atttcctaag taatatacat
     5221 gatgactctt taagaagctt aatttcagca ttacatgatg atgaaaatat aggtaaagat
     5281 aagctagatt ttctagaaag ttattttgat aatttaaagg atgaataatt atatgctata
     5341 tgtttttgaa aatcttataa agacaactat tgtatgtagt cttggtattt gtctattatt
     5401 atttttgaaa agatatttat ttaagaagtt tagtaagaga ttcaattatt atatttggtt
     5461 gataatagtc tttagaatgt tactttttct atttaattat acaatagtgt atgaagtaaa
     5521 agaacccaaa gaaaatgcat taggaaataa tattacacaa attgatattt ctacagataa
     5581 taatcttatg ctgtatgtag cttatttatg gttatttgta actatagtca ttgcagttta
     5641 tacatttata aagtatacaa gatttaaaaa tctagttgtt gatgtatctt atgatattga
     5701 agataatgat ataaactgta tctacaaaaa cttgttaaaa gaattaaata taaaaaagaa
     5761 aattgaatta agaggttctg atgaattgat aagtccagca ggtatgggat tatttaaatc
     5821 ttacatattc ttaccagact atccttatag taaagatgaa ctaacttgga ttttgaaaca
     5881 tgaattaatg cattttaaaa ataaagatat tttaataaag tttttagttt tgtctgttag
     5941 aataatatat tggtttaatc cacttgtata tgtaatgagc aataaagtaa atctagattg
     6001 tgaattatgt tgtgatgaaa gtgttttggt agaatgttct ttaaaagata aaaaagaata
     6061 tgcactagca cttataaagt caattaagtt tagtaaaaat tataatagta agattttgac
     6121 aaccgagttt gataagacaa atttggaaaa gagacttgag agtattgtta aaaagaaagg
     6181 caaaagtgga atactaattg ctgttttagt atttatgact tctataacat cttttgttga
     6241 agttgatgct caagttagaa ttgatgaggg taataatgga atgaataaca atattaatga
     6301 atttttattg aataagacga ctgataaaat tataaatatt tattataaag atgcaccaaa
     6361 tgaaattagg aaattttatg aagataaatg cgaattagaa ggtaaaatac ctagagacta
     6421 tgacataata gaaatggata tgaaagatta taatcaatta ataaaataat tataaggagg
     6481 attttatgtt aacaaaatta agaaaaatag tagctattat gtgttgctta ttgcttactt
     6541 ttacaagtgt ggcatttgct gacacaagta aagagaaagg aggaagtaaa gagactgttc
     6601 cagagaaaaa cctagattct tttactcttt ctgggtatat atatacatat gaaaatgcgc
     6661 cacaagaaat aagagatgaa cataaacaaa attgtgaaga gataaatata gaccctaagc
     6721 cagatgatga gatatttgtt ccagaatcag ctaatctaat gaatgaggac aatagtaaag
     6781 gtgtttatgc aagttatact gtttcttata atataggtgc taaaactata acaattatga
     6841 gtaatgagta tttgactata agtacaacaa aagttatacg aaagggtaat agcggaaaag
     6901 aagtaaaagc tgcacaaatc atgttaactt tacttggtta taatgtaggt atagatagtt
     6961 cttttggaag taagacatat aatgcagttg taagtttcca aaaaaaatac ggtttgtctg
     7021 cagatggaat aattggtcca gctacttggg ataaattggg tagactaact gaccctactc
     7081 tttcatagga ttaaaatcta aaaatatatt aaatagtgtc tcaatatgat ttttcaattt
     7141 aaatgagaca ctatttaaat ataaatgtta taaaactatt ttacaataaa ttccatataa
     7201 ctctaccatc tatatcctta acattttgac aaccagtcaa aatcatagtc tcacataact
     7261 cattcctaac cttctctata aaaaactcca caccatcaag accaccgcca aaagaagcag
     7321 taacaaaagg tcttcccatc aaaacagcat cagcacccaa tccaagcatc ttaactacat
     7381 caacaccagt cctaactcca ccatcaacaa gtattgttat ctttcccttt acagacttag
     7441 ctatatctgg aagtacctca caagtaccag gagtataatc aagtactctt ccaccatgat
     7501 ttgaaacaat aatggcacta gcaccagact ctacagccat caaagcatca tctacagtca
     7561 taattccttt tataataaaa ggtaatctag tggattcaac caattctcta atctcatcaa
     7621 ttgttttagg agaaaaagga ttttcttgaa attgattatt aataagtcca caagcatcaa
     7681 ggtctacacc aactgcaaaa gcacctgctt cttcagacaa tctaatttta tctattatct
     7741 ttgaattatt ccaaggcttt ataaatacaa ttccatttcc cctattatcc ttcaatacat
     7801 ctaaattatc cagtaaaaaa gtatctacat tagtatcgcc aaccatagca taaattccag
     7861 aattagaaca acctctaaca acaggctcta tgtattcttt ttcggaaact ttaccaccca
     7921 tgttcaatat agtacctgat acaggtgcag cgaatatagg agaactcatt tttctaccaa
     7981 ataattctat ggatgtatct ggctttgaaa cattatgtat gactctcata tttatcttta
     8041 ttttttctag actttttcta ttttcaatga aagacgaacc acttccttta ccacccatac
     8101 caggaacatc tccagcacaa gctaatccat tacaaatttt acatacttta catgttccat
     8161 taaagttctc tctagctgat tttagtaagt cattgtaatc catacaaaat ctctcctttt
     8221 tataatatta aaattgagta ttactatatt gaaaatatct taattgtaaa tatagggatg
     8281 ttattatgat gagctaaact agataataca tattagatat tttttattgt tgtagatagt
     8341 taaattatat catattagat agtcaaattt tgaaaaaaac taacttattt aagtatacat
     8401 tttgaaaaaa ttaaaaaagc tatatataga tttttaaaga aatctatata tagcttttaa
     8461 gtttatgtca atcttttttg ttaatgaata aataatatag aatacaatat acaaaaaagt
     8521 ttcttgaaat gctaagataa tccccattta caaagcaaca tcattatact gtgtaaattt
     8581 gaatcaacaa tgttttcacc atatttcatc ataaaatcct ctaattgtgt gtcaataaat
     8641 gaataatatc cacttatact atcgatgtat gcagtcatta agtgtataca ttcatcactt
     8701 ttatctttat acatatatat ctccccctat atacaatata taaatttaga taccaaatcg
     8761 tatcatatta aatagttaag tgtgtaacat ctatgcagtt atatatttat atcattatat
     8821 taataactaa agtgagtttt gacaatagat tttgggaaac tggatgttca aagtgcaaac
     8881 ttggatttta aaatcattaa ttaagatagc gcataggaat atatgtatac ataacttaat
     8941 tataaagatt ataaacaatt acttttatta actttattta aatggtagaa taacttggag
     9001 attaataatt ataaggagga tataatatga gtttatataa gtttatatat gatgataagg
     9061 aatatttatt aaaagaggag aactgtagtg ctttaataaa tgatgaagaa aatccagttc
     9121 aaggagtaag tatttcaaaa ataattgata tacttaatga agcagaagaa gttgattttg
     9181 atgtagaata ttatcaagaa gcatgtccat tatgtttaga aggagtaaaa gaaaagaaaa
     9241 aattctttcc ttttttagaa tatcactttt atattttcag taaagatgga gaatatgtaa
     9301 taagcaatat atctgttgat tataaaggac tatcttttaa taaactatca agggcaaata
     9361 aagttgataa tagttatatt gtaagtgtga taatttgcga aaattgtcag gattatatag
     9421 ttcagataga aaattgtata gtttagtgta aagtatgtct taatttaaga gaaatttcta
     9481 aattgagaca tcaacttttt tcaaaaatag tatggaaaat tactaaattt atgagataat
     9541 gtaagtcgga ggtgagttac aatatatagt aaatatttgg aagagataga tgatacaatt
     9601 ttagattcaa tgtttaaact aaccttgaat gaaagattat tgactaataa cgattttatt
     9661 gaaaataaaa ttgatgtagt aaagaattta attgattgct ataaaactga acaaaatagt
     9721 ttaatgttat taaaatcaaa ttttttgcta gctttattat atggtattca agggtttagt
     9781 gaaaaaatga aagagtatat tttgatttcc tgtaaatata ttgataaatg cttacccaaa
     9841 gattataaat ttttagcacg attttattca caaatagcta ttttaagttt aaaaaatgaa
     9901 gatgtaaata aggcaaattt atatatggat aaatttaatc ttatttgtga tgaaaataat
     9961 tttctaattg aagaaattat ttttaaatca caacttattt acattaaggc aagtaagtgt
    10021 atagaatctt ctgtaataat taaagaaata gaaacgttgt atataaagat aaaagaaatt
    10081 aacgatttta attgtaaaaa aatatatttt tttattattg gtaaaatata ttttttgttt
    10141 ttggatgatg ctctaattgc taaggtaaat tttttaaaag caagaaaata tgcagattta
    10201 ctaaaggata tgcaagtatc ttcactatgt aatataaaga taggtgagtg tgaatgttcg
    10261 tttgaaaatt atgaatgttc catggaatat ttcaatgaag taataagcaa taaaaagtat
    10321 agaaatgtaa atataattca aaaatataga gcatcaaaca acataactaa aatattaata
    10381 aagactagaa attatcctgt cgcaataaat aatttagcaa aatctgaagt atatctagaa
    10441 aaaattaaaa atcatagctt aaaagaagta gagaagtttg atttatttat aagtttagcc
    10501 atgtattatg caaaaagtaa tgaaaaatct tttgaacaat caatatgcta tttagataga
    10561 gctaaaaaca tattaaatat gataaaaaat atagaagaat atataatata tgatttagaa
    10621 ataatttatc accaggcaaa gatatattat atctttggat attatgacaa tgccttaaat
    10681 acaaataaaa aactccttga aaaatctaaa aatgctaaaa attataatta tgtgaagtta
    10741 gcatacaaga gtatatattt gtgttttgaa aagattcaaa attatgaaat gagtatgaaa
    10801 tactttaaaa tgtattatga gttaaaaatg atgtatataa aagctcgaaa tagaaattat
    10861 atagattcct taaattatag acatgagtat atagaaaaag aaataaataa catgagaaaa
    10921 gtaaaacttg atttagataa aaaaaagtat gttgaccatt tgacaagtgc atataataga
    10981 acttttttga acgatttttt agaaaaacaa gaagttagtg aatattgcac agcatttatg
    11041 atagatgtag attactttaa agaatacaat gatacttatg gacattataa tggagatatt
    11101 gctcttaaaa atataactat aataataaaa aaatatctaa aaaaagacat gcttatgata
    11161 aggtatggtg gagaagaatt tctgatatta tctgtatgta aagattataa aaaaagtaaa
    11221 atatttggaa gaaaattatg taggattgta aagaaatttt taaataataa tttgacagtt
    11281 agtattggca ttgatacatg taaaaatagt agtatagata taaaagaaat aattgaaaat
    11341 gctgataaag ctttatataa agcaaaggaa agtggtagaa atagatgttt gcattatcat
    11401 gattttaaga atttttaact gttgaaatag gattggtgat ttacttgaaa aggagtttaa
    11461 tagaagattt aaagagaaat ataaacaaga tagaatacga tatgttgact aaattctatc
    11521 taaatttgac agatttaaac tacatcttgg attatcacaa agacctttta aatacaaaat
    11581 tagaagataa tagtagagtc tatactttat atttaattag tcttataaac tatataaaaa
    11641 aagatagaga agcatcatac aactatttga tagagtcatt aaagtatatt aaaaaattgt
    11701 cattaaaaga tgattcagat ataatatcta aaatttatat ttatcttact ttatcagcta
    11761 tttctataag aaaatatgat aaggtaaata atttttattt ctcagcaaaa aaaattataa
    11821 ggcaaaatca tttaaatgag ttgttggtat tgttaaatat gagtttatgt gtggaaataa
    11881 gtgctttata tcagaccaaa acggatgtaa tagctttagt agaagaagtg tcttttttta
    11941 aaactttaaa aaataaagct cttgtaagta gggcaaatta tatatttggg agagtttatc
    12001 tatatttatt taataacttt ataaaaagta tggaatactt aattgaatgt ctaaaattgg
    12061 ctcaagaaaa taatttatac tctatagagg ctttttgtaa atgtataata agtttgtgtt
    12121 atttagagag tggaaataat gtagaagcta taaaatacat aaataatgta ttagatgttg
    12181 cacacagtaa taactcaata tcagtaactg aaagagtgtc tataaaaata gaccttatat
    12241 gggcatattt aaaagaagat atgaataaag aagcagaagt tattttatta aagactataa
    12301 aattaatgaa agttgttgaa ggtgtatata aggattattt atattcattt atatttttat
    12361 attcagctga aattgagtta aagaaagata attgtagttt tgaaaaggta aacacctatt
    12421 taattttatc aaaaaatata tataaaagtt ttggagatgt ttttatatat aattctaaac
    12481 tagattacat atataagttg tatggagatt tatatataaa atttggtaat atagatgaag
    12541 gtataagttg ttatataaaa ggtttaaatt tagttaaaaa gagttcattg aatcttagaa
    12601 aaatatctat atttatatgg gctaatagca aatggatatg agctaaaaaa agattttaaa
    12661 ctggcaatag aatattataa aagtatggat tggtattctg ataggtggga aaaaaataat
    12721 tcctataaaa catctcaagc aattcacaaa caatatgaac tcagacaaaa acaagagagt
    12781 ttaagattac tagtggatga taataagaaa ttagaaaatg atattttaaa agatggactt
    12841 actaaactat ataatagaag atatttggaa cagcagttgg atttatttaa tggaaccaga
    12901 gacaaaaata tggtcgcaat actggtcgat atagattgtt ttaaaagtta taatgataat
    12961 tatggtcatc tagcagggga taaagttata gttaaggtaa ctgatattat aagaagtgta
    13021 tttatagata ttaccgaaca cataatcaga tatgggggag aagaaatttt aatattagta
    13081 gattctatag aatcaatcgg tgttgaagag attgataata tagaattata tattaaaaga
    13141 gttttcaaac ttcttgaatt tgagggtatt gaacatatat attctaaaat aaaaaactat
    13201 ataactataa gtgctggagt aagtataaaa aggtgtcgta gtagaaaaga tgttgaaata
    13261 ttaattgaag atgcagataa gaacctatat aaatcaaaaa aagcaggtag aaatcaatac
    13321 attataattt aaaattgagg tgattttgtt gcgaaaaaaa ataggaatta tagcctctga
    13381 tattgaatta aaagaaagaa tagaagaatt atatagagaa gatgtagaaa atggaactat
    13441 aataatcgac atacttaacc tagacttaat ggaaaatcaa ggtcgtatac ttgttgaaaa
    13501 aggtgcccaa gctataattg gtagaggtgg aggttatagc ttagtaatag atacagtaaa
    13561 tgtacctgtt ataccaatga atatgaagtc aactgacctt ttaagagcaa tagaaatagc
    13621 aaagaaatat tctaaaaaag tagtattgat tcttggagac aatgaagttt cttttgacta
    13681 tgttggctgg agaaatgtca taagtactga aataactgaa gaatggtttg aatctaagta
    13741 tgagataaga agtaaagttg taaaatatat agaccaaaaa gatgaagttg taatcgtagg
    13801 aggaggtttg gcatgttctt ttgcaaggca atatggaatt gatagtgtat ttgcaactgc
    13861 tagtgatgaa tctataagag aagctgtaga atattgtaaa aaattattag atacattagg
    13921 tgaagaaaag tttaataatg aagtgttacg aaatatactt gatggtataa aagatggagt
    13981 aattgctata gatagtaatg gcagtataat tttgtacaat gaaagtgcaa aaaacatgct
    14041 taaagtagag agaaaatgtg cacttaataa atacatctta gatgtatttc ctaaaatgga
    14101 gtggatgtta gattgtttac atgaaaaaga ggacgtagaa gatagaaaga taagaaatat
    14161 aaataatcta attgttaata ctaggacaac attgataaaa gttgataata gtacttatgg
    14221 agttttagga ataatacaag atataaccaa gctacaaaac ttagaaagaa aaataagatt
    14281 tgacttgaat caaaaaggtc tctatgctag atatactttt gatgattttt tatttaaaga
    14341 taaattaact aaagaattta ttgaagaagc taaaaaaatt ggaaaaagtg attatacaac
    14401 tttactttat ggagaaagtg ggtctggtaa agaaattata gctcatagta tacacaacat
    14461 aagtaaaaga aaagataggc cttttgttgc tataaattgt gctactatag cagaaaattt
    14521 acttgaaagt gaactttttg gctatgaaga aggtgcattt actggtgcta gaaagggtgg
    14581 aaaaagagga ctatttgaac ttgctcatgg agggactctt tttttagatg aaataaatag
    14641 tctatccttt aatattcaaa caaaactatt aagagtaata gaagaacgtc aaataatgag
    14701 aattggttct gattatataa tacctctgga tataaggatt atagcagcaa cgaatgaatc
    14761 tttaaccgaa aaaatagtta tgggtacatt tagagcagac ttattttata gattgagtag
    14821 tttagagata aatatacctc ctttaagaga cagaagagaa gatataatac ctttgtttaa
    14881 caattttgta aatgaagtat taaaggatga tggtctcaat ggaataaaca gtatagatga
    14941 aaattttgtt ttgactaaag atgaaatgga taagctatat aattatagct ggcctggaaa
    15001 tgtaagagag ttaaagacaa tagctcaaaa atatgttgtg acaggtaaaa ttaaattgag
    15061 acaagataga gattttaaaa ctaaaaaatt acttcctaac tctgaagtag acaaatttaa
    15121 ttctgaaacg acagcaagtg tagaagtcca agatgaatct ataaatataa gtaagataaa
    15181 tgatggtaag ataagtatag atataaaaga agtaaataag tatgttgaag aaaaaataat
    15241 aagtatgctt tttgctcagg gactttctaa aaatgaagtt gcacaagttc ttggaataag
    15301 caggacatct ttatggaaga aatacaataa aaatatttaa atataattac atataaatta
    15361 ttttgaagta atgttaattt tttaaaacaa gtgttaagaa aaattaacat tacttttttg
    15421 caaattttta tttttttaga caggcatata tatttataag aaaaatagtt aaaacactta
    15481 atttaataat gaaaaaaata ataattctaa acaaaatcaa ttttacataa agtgggattt
    15541 attggcatag taattgctta atatataatg taagagtaaa tacaatttta ggggtgatgg
    15601 tatgagtcaa agtatggttg gtaagcatgc aatgtggcca aaagaaaatg atgttatatt
    15661 tagcatatct ggcagagctc aagcagcaga gaaggctttt ggtatggata atgtaataaa
    15721 tgcaacaata ggcgctctaa tggatgattc aggaaaattg ataacaatga aaactgtata
    15781 tgaagaatat aaagcattag ataactgtga gataggtgca tatgcagctc ttgaaggtca
    15841 accagattac ttagaagcag ttaaaaaagt atttttcaga gattacttac cagaaggaca
    15901 tataagagta ttagcttcac caggaggaag tggtgcaata aaattagctg tatggaacta
    15961 tactaatgaa ggagatgagg tattaacatc tgattggttc tggagtccat atgtaagtat
    16021 agctgaagag gctaatagaa aagtagttaa ttaccaactt tttgatgaaa atagaaggtt
    16081 taattttgag tcgtttaaag aaaaatttgt aaatatagca gaaaaacaag gtagagtctt
    16141 tacaattata aacacaccag cgcataatcc aactggatat agtgttgcag atgatgagtg
    16201 ggataaaata ttagacttat caaaagaagt cgcaaaagat aaagacaaaa agataatatt
    16261 tttcgtagat tcagcatata ttgactttgc aggtgatgat gatgtatgta gaaagttttt
    16321 taagaaattt tctaatttgc ctgaaaacgt tctcgtattg gttggattca gtatgtcaaa
    16381 aggatttaca gcttatggta tgagaatggg agctataatt tgtatatctt caagtgaaga
    16441 tgtagctgaa gagttccatt actcatgtgt tcattcatgt agagcaaatt ggtcaaattg
    16501 taatagaagt gctatggcag tattatctaa cattgtaaat gaccctaaaa aattcaaaga
    16561 atatgaggat gaaaaagaaa tatacaaaaa catgcttact agaagagcag atgtatttgt
    16621 aaaagaagct gaaagagtag gattagaaat tcttccatat atagcaggtt tctttgtaag
    16681 tataccatgt gataatccaa aagaagtatg tgaagaattg actaaacata atttatttgc
    16741 agttccatta aaaatgggct taagatttgc agtttgtgca gtttctgaag ataaatgtaa
    16801 gaaagcacca agtattataa aagaagcact tgaaagttta gaagtaaaaa taaataatta
    16861 atttcattga acaaattggg tggactatga atttaataat tgtagatgga gggaatttta
    16921 tatgaaacaa ttgatgacag gtaatgaagc aattgcacgt ggagcctatg aggctggtgt
    16981 taaatatgca tctgcatatc cagggactcc aagtacagaa atattagaaa atatagcaac
    17041 atataaagat gcaatagttg cagagtgggc tccaaacgaa aaggtagcac ttgaggctgc
    17101 tatagggggt tcaattgcag gagcaagaac aatggcttct atgaaacatg ttggtttgaa
    17161 tgttgcagct gatccaatat ttacttatgc atatacaggg gtaaatggtg gtatggtact
    17221 tattacagca gatgagccgg gaatgcattc atctcaaaat gagcaagaca ataggatgta
    17281 tgcaaaattt gcaaagatac ctttatttga accatcaact agtcaagaag ctaaagatat
    17341 gataaaagaa gcttttgaag ttagtgaaaa atacgataca ccagttttat atagagttac
    17401 aacaagactt tgtcattcaa aaggattagt ggagtgctat gatagagaag aagttgaaat
    17461 taaagagtat gttaaaaatg ctaagaaaat ggtaactgtt ccagcaaatg ctcaaataag
    17521 aaggggcgtt gttgaagaaa gaatggaaat tcttaaaaaa ttctcaaatg aaactgattt
    17581 aaactactat gaaataaatg atacaaaaat aggtgttata gcatctggta tgtgttgtaa
    17641 ttttgccaaa gaagtttttg gaaaaaatgc atcatatatg aaactaggat ttacaaatcc
    17701 attaccttat gaaaaaataa aagaatttgc agaaaaagtt gataaaatat atgttataga
    17761 agaaaatgac ccatttatag aagaacaaat aaaagcatat ggaatagatt gtattggaaa
    17821 agatgtaata ccaccatatg gagaaatgac accagatgtt ttaagaaaag ctatatttgg
    17881 aaaaacaaat gatactatag aatacaaatc tgaattagta acaccaagac caccaagttt
    17941 ctgtgcagga tgtccacata gaggattctt ctatgaactt ggaaagagaa aaaatttaat
    18001 agttggtgga gatataggtt gttatactct tggatttgct cctccatata atggaataga
    18061 ttatgttgtt tgtatgggtt ctgcttttgg aactgcacat ggagctcaaa aagttcttaa
    18121 tatgaaggat gataatgaaa aaagattggt aggtgtactt ggagactcaa cattcttcca
    18181 tacaggaata aatggacttt tagatgttgt ttacaataga ggtaattcta tttcggttat
    18241 attagataat agaataactg gaatgacagg tcaccaagaa aacccaggtt caggatatac
    18301 tttacaaggt gctaaaacta aagaagttga tatagaagga ttagttaaag cttgtggaat
    18361 agaacatgta agagttataa accctaataa tttaaaagaa gtaaatgaag cactagattg
    18421 ggctttagct atagaagatg aaccatcagt aataataaca agatggccat gtgtacttaa
    18481 aaaattctct aaagaagata tagaagaatt taataatcca tttaagacta agtgtaaagt
    18541 tgaccatgat aaatgtatag gatgtaaatt atgtttgaaa actggatgtc cagctctatc
    18601 atttgataaa gagaataagt tatcaaatat agatagaaat cagtgtgtag gatgtggagt
    18661 atgtgcacaa gtttgtccaa aacaagcaat agttaaggag gaaaaataat atgactaaga
    18721 gtatactttt ggtaggtgta ggaggtcaag gaactatact tgctagtaaa ttattaacta
    18781 tgggattgat ggaagctgga tatgatgtaa agatgagtga aatacatgga atgagtcaaa
    18841 gaggaggttc agtttcctca caagttagat atggagattg tgttcattct ccagttatag
    18901 aaattggtgg agcagatata ttagtatctt ttgaaaaaat ggaagcttta agatggttca
    18961 attatttaaa accagaagga aaagctttag tgaataatca taggatagat tctatgactg
    19021 ttttaatcgg aggagctgaa tatcaagaaa gtgatataga tgcagagtta agtagattaa
    19081 atgcaaaagt tataaatgca gctgataaag ctgaagaact aggaaattca aaaataatga
    19141 atgttatttt attaggatgt ctagttaaat ctatgcaatt agagtctatt gattgggaaa
    19201 aaattataag tgaaaatgtt aaaccaaaat ttgttgaatt aaacataaaa gctttccatg
    19261 aaggtatgga aatggttagc aaatagttta taatagatat aaatagacta tagaaattaa
    19321 ggtgaaagga agaaaattat gacttacaga atattagcca taaatccagg ttctacttct
    19381 acaaaaatag cagtatatga tggagaagaa caaattcttg tgaagacgat agaccatcca
    19441 gctgaagaga ttgcaaaata taatactata caagaccagt ttgaaatgcg taaggaagca
    19501 gttttgaata ttcttaaaga aaatagtata gacttaaaat ctcttagtgc aatagtagga
    19561 agaggtggag ttttaccacc agtaaaatca ggagcatatt tagtaaatga agaaatgatt
    19621 gatgtactaa gacatagacc agtacttgaa cacgcttcca atttaggtgc tgttgtggca
    19681 catgcaatat cagaacctct tggaatcaac tcatatattt atgattctgt tgcagtagat
    19741 gagcttatag atgtagcgag aatatctgga ctttgtggaa tggatagatc aagtgcaggg
    19801 catgcattaa atactagagc aatggcttta aaatatgcta aggataaagg aaaagattat
    19861 aagagcttaa acttaatagt agctcacatt ggtggaggag taagtattta tcttcatgaa
    19921 aaaggaagaa tggttgatat gctatctgat gatgaaggac cattttctcc agaaaggtca
    19981 ggaagagtac ctgctacaaa attagtggct gcctgttatt caggtcaata ttcagaaaga
    20041 gaaatgacta aaaagataag aggtaaaggt ggtatagttt catacctaaa tactgtagat
    20101 gctagagaag ttgaaaaaat gatagcagaa ggaaatgaag aagcaaaaat tatttatgaa
    20161 gcaatggctt atcagttagc aaaaggtatt ggagagttag caactgtagt agatggaaag
    20221 gtagatgcta taattataac aggtggaatt gcatattctg aaatgtttac ttcaatggtt
    20281 aaaaagaaag ttgagtttat agcaccagta gaaattatgg caggagaaaa tgagttggaa
    20341 tcacttgctt ttggaacttt aagagtacta aatggagaag aagaagctag aatttatagt
    20401 gaaaattaat aagaaaaact aaaaatgttt atatactaaa aaagtggtat atccgttttt
    20461 tatacacgaa agcttagagt ataggtgaaa atgtataaaa tttggattgc cactttttat
    20521 aattaaaatt ttatcttaac ctaataaata attctgtaaa atttcgtatt tatatatata
    20581 ttttattggt atttattgtt attattatta tgaggagtga tttttgatgg gaaaaataag
    20641 tttaaaaagt atgataagat tattttcagg tttttttata tttgcaattt cttctgtgct
    20701 tatgattaat gcacatgtag gtcttatgcc ttgggatgta cttcaccaag gtttatctat
    20761 aaagctaggc ataactatag gtcaggcttc tataatggta ggagttgtaa ttgttatatt
    20821 ggatgcagtt tttggtgaaa atataggatg gggtacttta ctcaacatga catttatagg
    20881 aatttttatt gatttagtaa tattttctgg agtgatacct catgcaagca acacttatat
    20941 aggtgtgttt atggttgtta taggaattat tttggcagct atagcaagtt ttctatactt
    21001 aggtgtttgt ttaggaagtg gaccgagaga tgggcttatg attgctcttc agaaaaaaac
    21061 taataaatct gtacgtcttg ttagaacaat tcttgaaata ctagcattag ttgttgggtg
    21121 gttattagga ggttctgtag gtattggtac gcttgtaagt gctttaggtt taggatatgt
    21181 attacaaata gtgtttagaa tatttaaatt tgacacaaaa ttgttgaaac atagatttat
    21241 tatagatgat ataagagaat ggaaggaaaa gaaaagcaat gaacataaat gtaaatcaag
    21301 tattgtaata aaaaatgagc agaattaatt atgctaaaat atatttgtag tttatctaat
    21361 ttatagtaaa gtcaaggaga ttttgaaatg gaattatggg atttatataa tgctgatgga
    21421 attaaaacag gaaatgttat aaaaagaggt aattctattg aagaaggtta ttaccatttg
    21481 gcagttgaag tgtggatttt aaatagtaac tctcaaattt taattcagaa aaggtctaaa
    21541 agtaaaaaga cattgccaaa tatgtgggga atgacaactg gatgtattgt ttctggggaa
    21601 gacagtttag aaggagcaat tcgcgaagca aaggaagaaa tagggataga tataactaaa
    21661 gatgagatga aagtttttag aagtatgatt catgaagata ctctttggga tgtatactta
    21721 gttaaaaaag aatatgatat atccaaagct attttacagg aggaagaagt aagtgatata
    21781 aagtgggtaa gcacagatga aataagacaa ttactaaaag agggtttgtt ttttgaatat
    21841 ccagagatat acgaattgct atatgagata gataataata aattaaatat atgatgtaga
    21901 gcagatttca gtataagtaa gattatttaa aatgtatctt ctctaaattt taatattgtt
    21961 ttatgaaaat taataagtta tttttagtat ttttctactt gttttttgag attaaaataa
    22021 aaagggcagt cattataaag tcataaaaat ttggggagga tttaaactat ggaagataac
    22081 aaaggattgg cacgtgacag ttcaaaatat aaaattgttt ctatatctgt cgtagtaatt
    22141 attctaatta cattttggta tatgttaaat atggtgcttt taacatttat tatgactttt
    22201 attttctata acttgcttgt agccacaaga aaacgaatta agaagttttc ttctttaaat
    22261 attccagatt cattaataat tatagtgtta tatgcactat ttgctatatt gttagtattg
    22321 attagctatg cagttgtacc aataattata gttcaactta cagagttgag tagggttttt
    22381 agtgattttg atgtaaatca atttgctcaa tctttaggac caaaattgta tcctattgtc
    22441 tcaaagctgg attttaataa atatatttca caagcagggc tacttatagc atcaacagca
    22501 acaaaagttg gaagttttgg agtaaatata ttattggcat ttttactgag tttactcctt
    22561 ttacttgaaa aaaatgaaat taaaaatttt ggagataaac tttctgatag taagatttct
    22621 tttatttata attcgcttgt attttttgga aagagctttg ttaaaaattt tggtgaagta
    22681 atgaaagtac aagttatgat tgcctttata aattctgttg tttctatgat tttcttaggt
    22741 ttcatgggat tccctcaaat atgggcttta ggatttatga tttttgtgtt gggtcttatt
    22801 ccagtagcag gagttatagt gtctttgatt ccattaactg taatagcatt taatacagga
    22861 ggaataacaa aggtttttgg agttttactt atgatttgta tagtgcatgc agtagaaaca
    22921 tacatattaa accctaagtt gatgtcgaat agaactaaac ttccagtttg ttttgttttt
    22981 attattttac ttgtaggaga acattattta ggtgtatggg gattactaat tggagttcca
    23041 atatttatgt tccttatgga tattctgggt gttaaattta cttctaggta atatattaaa
    23101 ataataattt atctttatat ataaattata aattgtttta aaaaagaacc tataaataga
    23161 catcattaat aaagacatct atttgatagg ttcaattttg ctttgtataa atactataaa
    23221 actaaataaa aagaaattta tttcatttca aaagtttcac aacttgcatt tctttcattt
    23281 atatgaactt tttcagcttt gcaaagttca tttttattgt atttacattt tacagcttca
    23341 caatgaatgt tatctgtatc aacttggtta gtttcattag aacgactagt tataccagat
    23401 gtatatttgt cttcaaaaga agcacaataa gtattttctg tagtagttgc ttgcattcca
    23461 tctactttta tactaccagc ataacaatat ccactagtat tataagcaca attagtagca
    23521 gcacaattta aattattatt tttcataaat aacctcctaa gttttattta aacttattat
    23581 gtgaagttat ctaaatttta tacacttatt tacaattaca tttatcacat ttaacaacta
    23641 catctttgga agtttccctt cctaaatttt tattaacatg atacacatca acattttcag
    23701 ttgatccaca tatagaacat ttaaaattgt tttctagtag ggttctttct ttaaataact
    23761 tccaatacat agaattgatg taatcattat aatttacctt gaggcatgtc tcaagaaagt
    23821 ttatgtatag attgtctaaa tctcctgtat ttaattctgt atattctggt tcaaaatatt
    23881 cacaacatcc tctaaaatcg ggctttgtat ctgaaataac acatctaata tcatatctat
    23941 agtctaggct aggcccctta aatgaaagat gtgtgcaaaa agaacaagat gtttctggta
    24001 gatttttttc aagttcatgt aatttattat tcataagtat tcctccaaaa aatttatttt
    24061 aagtatataa acgaatagat tattttaaaa cttttattta tttatattag agatttagga
    24121 taaatattcc aataaggagt aaatacttaa gtatttatta ctgtattaaa taaaaaagag
    24181 gcaaatctaa cttgagattg cctacttttt tatcattttt taataaaatt tattctaagt
    24241 attattttat ttagatttag tcagccaact tagtatctgc tttgcttttt aactttctac
    24301 cagagcgtac agttaaaata gcatataata cagtaaatat ggcagctaaa acatatgata
    24361 tatttatgtt taagttaaat ccaatttgag cattaaatat ataactgaat ataacaaagg
    24421 aataaaacat accaggaatt aagcttataa tataattctt ttcttttatt ataaggtaaa
    24481 ctgtaatcat tgcaaatgca aatatggcaa tagtttgatt actccaagag aagtatctcc
    24541 acaatatatt aaatccagaa gcactaagct ttgcaaatat aaggatggct atgacaggaa
    24601 taaatataca gattgacaat ataacacgat gttttttttc cttttggtca taatgaaagt
    24661 aatctgcaag cattaatctt aaagatctta aagctgtatc tccagaagtt attggtaaaa
    24721 caattacacc tattatagct atgataccac caatagaacc aagtaaatct cttgcaacca
    24781 aacctattac atcaggagaa cctactaatt ccttaggtat tcctttattg taaataccca
    24841 tagcagctgc agcccaaatc attgcaatta aaccttctaa aatcatcata ttataaaaag
    24901 tagtttttcc ttctttttca tttgaaactg atcttgctat aagagtagct tgagttgagt
    24961 ggaatccaga aacaatacca caagcaactg taacaaagaa tgtaggaata agaggaatac
    25021 catcaggatg aatacctttc caatttgcaa gacttaaatt agctaaatca taaccttgtg
    25081 taaagattcc aattccaaca ccagcagcag ataaaagtag aatagctcca aatattggat
    25141 atacctttcc tatgatttta tcaattggaa atagtgttgc acacaagtaa tatagaaaga
    25201 taagtccata aactatccag acaactggat tatttatagt cgatttcata tttaaaattt
    25261 gagtaacaac taaatcccca ggtgtataaa taaaaactgc accaactaaa agcattaaaa
    25321 gacaaaggaa tatattatag acttgaaata ctttattacc taaaaatcta ctgataagag
    25381 aaggcatttg tgctcctttt tctcttatac taatcattcc actcatatag tcatgcaaag
    25441 ctcctccaaa tacacagcca ataggtatta gtatgaatgc tattggacca aacaatatac
    25501 cttggatagg tcctagaatt ggtcctgtac cagctatatt aagcaactca attagactat
    25561 ttttccattt tttcattggg acatagtcta ctccatcact ttgagcaagt gcaggtgttt
    25621 ttctgtcatc tgggccaaat actttttcac aataagcacc gtataaagca cctcctatta
    25681 gtaatatagc caagccgatt aagaatgttg tcataatatc accccattta taaaattttt
    25741 tggtctttgt atacatatta tctaaatgtg tagatttata ttgaatttaa ttctggacaa
    25801 cctacaaatt ctataaagta ttacaactaa tataatttca gaaataaata catatacata
    25861 accatgtact tagtatacag gtatttggta atattttcaa tgttatagca ataaagaata
    25921 tagacaaaat tctgataata ttttacaaaa aaattaaaaa atataaaaat tagtttaaat
    25981 agatggagaa aaaaggaaaa tcaattgaga atataaatgt ctatatctta acaatattaa
    26041 gaggtttaaa attcttgaag aaagaatagt caagtgatat aatgagttta caggtgtata
    26101 tacactagta catatagaaa gcggtgcaat tatggataaa gatttaacat atcaaatcaa
    26161 agagctaaag aaagaaaaaa atgcaataat acttgcccat ttttatcaac caccagaaat
    26221 acaagaatta gcagatgcag ttggtgactc atattattta agtgagattg ctagagattg
    26281 taaagaagaa gtggttgtat tttgtggagt aaggtttatg ggagaaagtg ctaagatatt
    26341 atcacctgaa aaaactgtat tgatgccagt aagtaacgct gggtgtgcta tggcagatat
    26401 ggtagatgaa gaaggcgtga taaaattaaa acaacagtat ccaaatgctt tggttgtatg
    26461 ttatataaac tctacagcta aagttaaggc acattgtgat gtttctgtta catcttctag
    26521 tgcaattaaa atactagaaa acatagataa taaagaaatt atattccttc cagataaaaa
    26581 tttaggagga tatattgcag aacaatttcc agataaaaac tttatatttt gggacggata
    26641 ttgtaaatac cataacaata taagagctga agaaataatt gagttaaaag ataagtataa
    26701 aaatgcagaa gtattagtac atccagaatg taaaaaagaa ataagagatt taggagatta
    26761 tgtaggtagt acatcaggaa taataaaata tgctactaat agtaaaaaca aagactttat
    26821 aatagcaact gaagaaggta tattacatga attaaagaaa aataatccta ataaaaactt
    26881 ttatattcct ggtggaaaaa tactatgtac agatatgaaa aagacaacat tagaaaattt
    26941 atatagtaca cttaaaaata tggaaaatga agttattgta gaagatgaaa tcatggaaaa
    27001 ggctttaaat tcactgctta acatgcacaa attagcagaa ggatagaggg atagacttat
    27061 gaatttagaa caagatgttt taatagttgg aagtggagtt tcaggattat actgtgcatt
    27121 aaatttagat aaaagtttaa atgtgctagt agtatcgaaa tcaacaattg aaaacaacaa
    27181 tacatatctt gcacaaggtg gaatttcaac tgctagaaat attgatgata tagaatcctt
    27241 tgtagaagat acaatgaagg ctgggcagta taaaaataga gttgaagcag ttcaagtact
    27301 tgcagatgaa tctatagaaa atgtagggca gatagtagaa tatggtatgc ctttagacaa
    27361 agaaaatgga gagatagatt acacaagaga aggagcacat agtgtaaatc gaatagttca
    27421 tagtaaggat aatactggag aagttgtttt taaaacttta ttaaaagaag ctaaaactag
    27481 agaaaatata actcttatag aagatgccta tcttttagat atattaaaag atggcaataa
    27541 atgtatagga gctagaattt ttaaatctaa aaaagagatt catgtttttt caaaaatagt
    27601 agttttagct acaggtggaa ttggtgggct ttttaaaaat tctactaatc aaagacatct
    27661 tacaggtgat ggaattgcaa tagctttaag aaataatata aaaatagaaa atttagaata
    27721 tattcaaata catcctacag ccttttatga agaaaataat gaaggtagga gaatgcttat
    27781 atctgaatca ttaagaggag agggagctaa acttttaaat aaaaataaag aaagatttgt
    27841 ggatgaactt cttcctagag atgtagtttc aaaagctatt tttgagcaaa tggaaaaaga
    27901 taaattacct tatgtgtatt tagatgcaac acatttagat agtgaatatt taattaatag
    27961 attttctttt atttataatg aatgtttagc tagaggaaca gacataacga aagaatgtat
    28021 aaaggtatct cctgcacaac attattttat gggcgggata catgtagatt tggattcaaa
    28081 aacatctatg gaaaatttat atgcagttgg tgaaattagt tgtacaggtg ttcatggagc
    28141 caatagattg gcaagtaatt cattacttga agggcttgta ttttcaaaaa gagcagctaa
    28201 aaatataaac tcagttatag ataatgtaaa agttaagttt atagatgtac cagatatgga
    28261 tatagatata gaacaggtaa aaaaagaaaa taaaatatta gtcataaaag aaattgaaag
    28321 aacaagtgag gattttggcg atgaattatt tgattattga tagaatgata caggatgctt
    28381 tgatagaaga tgttccaagt gaagatataa caactaattc tattgtggat gaaaattcta
    28441 aatctactgt agatttaatt tgtaaacaag atggtataat agctggctta ggtgtattta
    28501 agagagtttt tgagatactt ggtgatgtag atgtaaaatt atataaaaat gatggtgata
    28561 gagtaaagaa tcgtgaaaag atagctgtat taacaggtag tactagaaac ttacttgtag
    28621 gagagcgtgt agcattgaat tatttacaga gaatgagtgg tatagctact attacaagta
    28681 ggtatgttga aaagcttgaa ggtacaaata caaaattatt agatagtaga aaaacgatac
    28741 ctaatcttag aatattagat aagtattctg ttaaagtagg tggaggatgc aatcatagat
    28801 ttaatttatc tgatggaata ttgcttaaag acaaccatat tggggctgct ggtagtgtta
    28861 agaaggcagt tgaattagct agaaaaaata cttcttttgt tagaaagata gaagtggaag
    28921 tggaaacttt agacatggta aaagaagcaa tagaagctaa tgcagatata ataatgttgg
    28981 ataatatgag tttggaaatg gcaaaacaag cagtagatat tataaatgga agagccattg
    29041 tagaattttc cggaaatgta aatttagata ctatagaaga tataggaaaa attggtgtgg
    29101 atgttgtttc tgtaggagcg cttacacatt ctgttaaagc tctcgatata agtatgaaga
    29161 atttgagaaa tatataaatt ttatattgaa ataaacttaa ttaaaataga ctccttaaat
    29221 ttgttttata aatttaagga gtctattttt tattcattaa aattaataaa aatagaaaaa
    29281 aatcaatcaa taatttaata aaaatagata aatactatat acacttttaa taatatagtt
    29341 aatatctatt ttacagataa ataagaataa acttataata taattgtatc tgtaaaattt
    29401 tgtaaaatat aaaaagtata gggggaataa aaatgaaagt agcttatatt tttagtagta
    29461 ctaatacaca taaaattcta gacaaaatga taataccaca acttgaacaa ggaactcatg
    29521 gagtagacgt tctagggatg tttttcttta tggataatac cttattttta tctaaaggta
    29581 atcctgtagg cgagagacta agtaaattac atgaaaaaac tgatatgata ataatggctt
    29641 gtgaccaatg tgctatagaa agaggtatag agaataattt ggttgatgga gctacaattg
    29701 gatgtttccc aaacctatat gcttgtttag gcagtgcagg tgtggaccaa gttataacgt
    29761 tataaattag tataggatac aatccataga aaaatttatt ttctgtggat tattttaagt
    29821 aaaagataaa ttgtttaaag taggggatta acaaatgtct aaaattttag ttgaatttat
    29881 aaacacttga ccatcttgtg atgaacatgg agtgtttata aaaaaaatgt cagaaaaata
    29941 tgctgataaa ttggaaataa aattatatca agcagggaaa gatttttcgt atataaaaaa
    30001 atatgggatt ataacaaaag gaactttaat aataaatcag aaaaagaaat atgatagatt
    30061 aaataaagat acaattgaaa gggcaatagt agaagctata aacaataatt aaaagtttaa
    30121 aaattaggag tatcaaaaat gaatatatta gaaattgttt taaatggagt taaagatact
    30181 atagtagctt ctatagttac actgaatgga gcatcaggat ggcttatgat aagttttata
    30241 ttggcaggtg tacttcacaa tgttttaact ccaagaaggt ttcaaaaaca acttggaaat
    30301 aagaaatttt cttctttaat taaaagtact atctcaggaa tgtttttacc aatatgtagt
    30361 tgtggagtaa taccacttgg aataagtatg tattattcag gagcatattt aggaccagta
    30421 cttgctttta tgacatcaac tccaataata aatccaatag ctgtagttct ttgtctaggt
    30481 cttttaggtc ctaaagttac aatcatttat ctaatagctg gttttttagt accattttta
    30541 gtaggatttt taggaaattg ttttggaaaa gaagaattat cacttcataa tgattcagag
    30601 gaagaagtaa tagaattaga agaagaaaaa agaagtttta tgggaaatat gattgatggt
    30661 cttaaatggt cttttggtga attagccctt actattagta aatatgtagt aatgggtatg
    30721 gtttttgcag gatttataac aactattttt ccaaatagta taattcaaaa atatttagga
    30781 aatccaggtt tgctatctct tggaagtata gcaatacttg catgtgttat gtatgtatgt
    30841 gctgtaggac atataccgtt tatagcagcc cttgttgcaa gtggagcatc tcctggtata
    30901 gcgataacat ttctaatagc tggagcagct acaaatctac cagaacttat aagtatgttt
    30961 aaacttattg gaaaaagaac tgttattata tattctgtaa ctttgacact gtcttcaata
    31021 ataattggat atataacaaa tttactttta gtgaataata actcagtaaa tttagatgca
    31081 acaaattctt caatacagtt ggcaaataaa ctaatgttaa atatacctga tagttttgag
    31141 tatgtatgta ctattttaat tattttattt gcatttaaat caataatacc aaagatgaaa
    31201 gaggtcatag gaaatcatgc ttaaaaagat agttataata tcctgtatag tagttgtgtt
    31261 aatcatttta tcaaaaatag ttgatgataa catcaaagaa gatgcttcta ttccaaatgt
    31321 gaataaagaa acattagaat attttagaaa aaattataaa gaagacataa taacttgtgc
    31381 tgaagaagac ttaaataatg atggaaaaaa agatttagta gttatatata aaaaaagcaa
    31441 taattcaaat gaaatggtag tcgtagtttc tgataaaaat agccactata taacaaagcc
    31501 aataccagca cctatagaga atcaaacgat aacctttaaa aatattgatg ataaagcacc
    31561 aatagaagta attgtatcag ggtctaaaaa tggtaatgta ggatatgcta tttacagagt
    31621 agagggtaaa aaatttgttg atttatttgg cgaagatatg gacaaatgtt gctaaaaata
    31681 aaaataatac ataaaggtgg gaaatttata tgaaattatc taagaagatt caagcattaa
    31741 tcttatttgg tgtaatgggt acatctatat taacaggatg tagtagtaaa ccaaaagaaa
    31801 aagaagaagc aaaaactgga aaagatgatt atacaataac agtaggttat tataattgtg
    31861 accatatgac tgcgggacca gtagcagaat cggcaggaat atataaagat ttaggtctta
    31921 atgtaaaaac tgttggaaat ggtaaagttc ctgaagctat ggcagcagga aaaatggatg
    31981 caggttacat aggaactaag gggttagttg gagcaatacc taagggttcg ccaataacaa
    32041 tagcagctaa caaccatact ggtggttctg aatatttaat agtttcaaaa gatataaaag
    32101 aacctaaaga tttaataggt aaaaaaattg caacagatat gagtgatttc ttatggacaa
    32161 gtgattatgg tccagaaaca ggtctaccaa ctgacccatc aaaatatgaa gttgtaaata
    32221 tggattcaga caaagataaa tatttagctc ttaaaactgg gaaaattcaa gcttttacat
    32281 cttgtgaccc ttggggttct gtagctgaaa atgacggtgc aggaaaaata atagcatcta
    32341 ctcaatataa agaaaaagct aatggcaaag aatataactg ttgttcattc tcattaaaca
    32401 aaaactttat aaaagaacat ccagatttag caaaaaaatt ggttctagca catacaaaag
    32461 caatagaata tatatataca aatccagctg aagcagctaa aatatttgca aaatattata
    32521 atgtagaaga agaagttgca cttagaacta tctataagaa gactgttgga gaaggtagaa
    32581 ctcttacttg gaaagttaca ggtgaagaat ataaaaataa tttacaaatg tataaagatt
    32641 taaaggcttt agatgatata cctaaatatg aagatacgat tgatacaagc ttattggact
    32701 cttgtggagc tgatgatttt gacaaattta taaaagaaaa agttaaccca gagtttcctg
    32761 aaggtatgag ttatgaagac tggaaagcaa aggtattata aataaatgga gaatatgaaa
    32821 aatataataa tactttttat cattattttg ggaagtatat ttataatatt ttttaatttt
    32881 gaagataaag aagaaataaa aggaaaatta agaatagggg tatctgatga tacctctggt
    32941 tttgtaataa attatatggt aaatagagac tactttgaaa atataaaagt aagtgatata
    33001 atggatattt ttacaattaa tgattgctga gcaagtacaa tgcagtgggc tctgagttca
    33061 gaggaaatag atatagcaat catatgtaaa gatgctgcaa aacaatatgt aaatgtaaat
    33121 agtggatttg aaattgttgg tactgtagtt caaaattctg atatatttct attaagtgat
    33181 aataatcctg aaaagatagg agtcattcaa aatagagatt accagtcaga attggttaaa
    33241 aaatactaca agaatgtgga agtagctccc ttattaggga ctgcactagc atatggatta
    33301 gaaagtaatt tagtagatgg tgttgtaata gattgcataa agtcaattgg gttagagggt
    33361 aaaagaaaat ctacaactaa attaggagat tatgatacct atgtattagt tgtaaataaa
    33421 gagtttaaaa aaagtaaagc atatagtgat tttataacac tttacaataa aagtgttgat
    33481 gctttaaaga ataaagctac atttaagaaa gtattaaaag agtacaggaa tataagaaat
    33541 atagatatgg aggaaataag caattggaaa ttaaaatttc taaagataaa agcgatagat
    33601 taactaagaa aaaaactgat ggtagtatgg atattttata taaaataata ctattagtta
    33661 ttatattttt agtctggcat tttgcagcaa aagatatagg gagttctttg ttacttccaa
    33721 tgccagtaga tgtaataaaa ggtttctttt tctgtgtgac agatgcagag acagtcacta
    33781 atctttttat aacattgcag agagtactta aaggttttat gtatgcatta ttgtttgggc
    33841 ttccaatagg atttattatg ggattttcta aaacttttga aagggtttta tctccagtag
    33901 tagattctgt aaggcaagtt ccaataatgg catgggttcc tcttactata gtatggtttg
    33961 gtataggaga tggtccaaca atatttttaa tagctttttc aggggttttc cctataattt
    34021 taaatactat tcaaggagta agagcaatat caaaggatta ttataatgca gctagaagta
    34081 tgggagcatc gccaattgtg atatttacaa atgtaattgt tcccgcatct cttccagata
    34141 tactaactgg ttcaaggatt gctataagta caggatggat gtctgttatc tgagccgagt
    34201 ttatagctac gagtgccggt ctcggttact caatggtaca agctcaaaca ttaatgcata
    34261 cagatgtatt aattgggctt atgatatttg cagctttaat aggatttata atagataggg
    34321 tattaaagtt tataaataaa atcctttgta gatggaggtt tgcagattaa tgaagttaag
    34381 tgtaagagat ataaataaga catttgtaaa taatagagta catacaaagg ttttagaaga
    34441 tataagtatg gatatagatg atggacaatt tgtctgtctt ttaggaccat caggttgtgg
    34501 aaaaacaact ttacttacta taataggtgg gtttcaaaaa tcagaacgtg gagatgtttt
    34561 tataaatgat aaaagagtta aaaaaccggg catagataga gcttttatat ttcaaaatta
    34621 tgctttattt ccttggaaaa ctataagagg taatgttcta tttcctatga aacagcagaa
    34681 aataccaaaa gaaaaaagag aagaaatgct agaagaactt ttggttatgt cagacttgaa
    34741 aggaaaagaa aaactgtttc cgcatcaatt atctggagga atgaaacaaa gagtggccat
    34801 gataagagct ttagcatgta atccagaggt attactaatg gatgaaccat taggagctgt
    34861 agattttcaa atgagacaaa atttacagga agagctagaa cgaatatgga taaagaagaa
    34921 aataactgca ttaatggtaa ctcatgatgt tgatgaagct gtatatatga gtgatagagt
    34981 tatagtaatg tcgagagata aaggaagaat tattgatgat ataaatatag atattccaag
    35041 acctagaatt agaggaagcc aaaaatatga agagtacaaa aacaaattaa ctgatacttt
    35101 atcaaagtgt tatgaagttt aaagaggagt agacatgagt caaatagaaa aatacaatag
    35161 tttggataat acacaacaga aaataagaat ctctattatg gatatgataa tagataaagg
    35221 ttctagtgtt acacttcaag aagtgactga atatgtaagt aaaaagttga atatagaaaa
    35281 agaatatata gaaaggacac tacaatattt tatttataaa aatataatgg ttgttgatgg
    35341 gaatagtata aattttatat atcctgtatc agcattgcca acaaatcata aagtaacttt
    35401 gagagataat agaagtttca gtgcaatgtg tgctatagat gctataggaa cctcttgcac
    35461 attcaaccaa gatataaaaa taaactctat ttgtagtgct acaggtaagg aaatagaagt
    35521 aattataaaa aatgaaaaga ttgagtatgt gaataatcca aacttaagag tgcttcatat
    35581 aaatcttgat aagcatctaa attgggctgc tagttgctga aatataatga atttcttctg
    35641 gacgaagagt gattttgatg cttggacaaa cgaagcggga ttatctgatg atgaagatat
    35701 atattgttta gatattaatg aggcaatagt agaatcatat aaaatattta aactcaaaca
    35761 aaaggtacta tcatagatat acatatatga aaaactaaaa atgaccttta gataaaaatg
    35821 ataggtaata tccatactat ataaattagt atagagtagg gatgtgaaaa gtatttaaat
    35881 ctaaaggtat atttttagtt tttttagatg attagatagc cttattaagt atatatttat
    35941 ttataacctt tgcaactcca ttttcattat tagtatcagt aacataatct gcaatttcct
    36001 ttatactatt acgtgcattc cccatagcca caccaagacc tgcatacttt atcatatgtt
    36061 ggtcattttc ttcatcgcca acagcaatag cattttctat aggtatattt agattcgtac
    36121 ataatgtttt aagagcagtt cctttattag tagttttacc taaaaattct agataaaaag
    36181 gagcagacct tactatagta tatttattga ataaattttt aggtagttgc tgtatgattt
    36241 cttccaatct ttctggttca tcaactatca taattttaca gattttaata gattcatcaa
    36301 ttttatcttt ttgtatatat ttgacttcta tattatttaa tttcgcttca tgaagtgtat
    36361 atttgccagg cgtttcattt ggtgtaatta aatttgtcat tgtatttatg tgtattttag
    36421 tcttatattt tttacaaacg ttattatata taaaatcaaa atcatctaaa ctcatttcta
    36481 catcatgtat gattttctca cttttaactt cttgaataat tgctccatta aagcatataa
    36541 cataatcacc ttcatctgtt aaatttagtt gatttagaag ttcttttaca ccaggaagag
    36601 gtctgcctgt tgttataact acttttgccc cagccctttt tgcctcctga atggactcaa
    36661 agacttcagg agtaatcctt ttttgtgtat ttaatattgt tccatcaatg tctagtgcga
    36721 ttaatttgta catatcaacc tccaataaat tatatactca aatttcctgt gaaaacgttg
    36781 acacagctag tgttgaacaa agtttcagtg ggaaatatgg tttgttttgt atactattat
    36841 atcaagagat aagtaattta gaaagtttaa taatattaat tttttgataa aaatattact
    36901 tatgtgagat gagaatgtaa aaactttgta atcaataatt ccaaaattct taattacaaa
    36961 taatggtatc gtttgcattg ttaatataaa taaatgtata ttatatagtt agaagtttga
    37021 ataaatagac tgatggtaat ttgttatatt agttagtttt gtgttataaa atattgtaat
    37081 tccattaatc atatgttatt attcaacatg attataatat aaagaatatt tgatatatat
    37141 aataatttat aaaagagtat tacatagtac atataaatct atatttataa atacaataaa
    37201 gtaaagttgt aaaggagtgc tttaaatgaa gttttcagag tttaagtatg aaagacccaa
    37261 ttataatagt atgaaaaaag agtttttgag ttgtgtagaa gatataaata attctcgtaa
    37321 ttataaagag caacaaaaaa atatacataa gattaattta ttaagaaata aaatagaaac
    37381 gttgtcaaat atagcttcga taagatatag tacagacaca tttaataaat tctacaaaga
    37441 agaaaaaaat tattgggatg aatatatgcc tttgtatgaa gaattaaatt catattttta
    37501 taatgccatt gtaaattcaa aatttaaata tgatttgata aaagagtttg gagagcaatt
    37561 tttcacaata gtagagtatt ctttaaaaag tttttccaaa gaaataataa gtgaattgca
    37621 ggaagaaaat aaattgtgtt cagagtatac tagactttta gcttcagctg agataatgtt
    37681 tgatgggaaa ataagaaatt tatctggtat ggggaaattc atgtattcta agaatagaaa
    37741 aacaagagaa ttagcaaaca aagcatatta taatttcttt gaagaaaatg aaactaaatt
    37801 tgatgatata tttgataaat tggttaaact tagagataag atgtctaaaa aattaggttt
    37861 tgaagatttt gtagagcttg ggtacattag aatgatgaga agtgactaca gagaatatat
    37921 gataaaaaat gttagaaaac aagtactaaa atatgttgtt ccaatggcaa atgagcttta
    37981 tgaaaaacaa gctaaaagaa ttggtctaga atatttaagt tacatagatg aaggcgtgga
    38041 atttttaaca ggaaatgcaa gtttaaaggg agattcaaga tacataatta aaaatggtaa
    38101 aaggatgtat tctgaactgt caaaagaaac aaatgaattt tttgatttta tgttagaaaa
    38161 tgaacttatg gatttagaaa cgaagaaagg aaaaggagct ggagggtatt gtacttatat
    38221 accagattat aagtcaccat tcattttttc taattttaat caaactgctg atgatataga
    38281 tgtattaaca catgaggcag gacatgcatt tcagttgtat atgtctagat ggattgatat
    38341 gccagaaatt aattttccaa cgcttgatag ttgtgaaatc cattcaatga gtatggaatt
    38401 tataacatgg ccgtggatgg atttattttt taaagaagat acagataagt acaaattcac
    38461 tcacttatca tcttcaatta aatttatacc ttatggagta attgttgatg aatttcaaca
    38521 ctatatttac aaaaatccta atgtagataa gtcaaaacga aaagaaatct ggagattttt
    38581 agagaaaaaa tacttacctc atagaaaata tggtgataat tcttttttag agagaggttg
    38641 ttggtggttt aaacaaggac atatatttaa aaatccgttt tattatatag attatgtatt
    38701 agctcaaatt tgtgcgttac aattttggaa aaaaatgatt caagatagag atgcaggatg
    38761 gaaagattat ataaatatct gtaaagttgg aggaacaaaa tcatttttag atattgtgag
    38821 tatgggaaat ttgtattctc catttgatga tggctgtatt gagtctatta taggtgatgt
    38881 taaatcttgg tttgatgaaa taaatgacag caaattataa aaaattatga gaattttatt
    38941 gtataaaagt aaaaaattaa aaaatatata aaaagtgatt atttgttgtg aaaatagtct
    39001 tgtttttaac taaattgata aaataaacta aaaaagattt gtacttaaaa aaaaaagaaa
    39061 atacaagaaa tatgcaatat caacatttgg aaaaaaaagt aaaaatgtat acaaaataca
    39121 tataaaaaaa ctattgcaaa ttttatagaa tgtttatata cttaagtcaa cagagataga
    39181 aaaaagctat ttatagaaaa caaaggataa attgcttaat tttaaatttt ctgaaaaaaa
    39241 taataatccg gatgaagagt atgggagact gctttataaa aagcgaccga agggacaagt
    39301 ttaatcttac ttacccaaaa gattagatga aattctcagg taaaggtgac tgtacttgga
    39361 cggggctctg aaaatgattt taacagggaa gacaaattgg gagatttgtt tttcctgttt
    39421 tttatttatc aagtaataaa taaaagtttg aaagagagta aaaaaatgat aataattacg
    39481 aataatccaa aagtaaaaga agaagttcaa ggcagagaag ttttgtttaa agacacaact
    39541 tatattggaa tcttagaagc aagtagagat ttgatacatg aaggttatga acttttatct
    39601 catccactct atgggagtgt aaagccaaat gaaacacctt acaggactgt tatactaaag
    39661 aaaggaaatc gtttggatat aaactcttta accttaattg aagaagcaat catcactgca
    39721 agtaaatttc agaataataa aaagactcca aaatggacag aaagtgttca agatgatttt
    39781 agagtaattg attatgatat attttataat acaatacaaa gaatgcagta tgaataaaaa
    39841 aattgttaaa aagttattaa aacagcctaa atattaaatc taatttaata ttaaataaaa
    39901 tgagaaattt aattggggag gataagtcaa aatggaaaat gtgtatgact tagtaataat
    39961 tggttcagga ccagcagggc ttgcagcagg tctttatgga gcaagagcaa agttaaaaac
    40021 tctaatccta gaaaaagaca agactggtgg acaaattgta ataacacatg aaattgcaaa
    40081 ttatccaggg tcagttccaa atgcaacagg accaagttta atagctagaa tggttgaaca
    40141 gtgtaaagag tttggagcag aaatgctaag agacaacatt gttgatactg agctagatgg
    40201 agatataaaa gttttaaaag gggaaaaggc agaatataga gcaaaagcag taataatagg
    40261 aacaggagca acaccaagaa agattggttg tcctggagaa aaagaactta ctggtaaagg
    40321 ggtttcatat tgtgcaactt gtgatgcaga tttctttgaa gattttgaag tatttgttgt
    40381 aggtggagga gacagtgcat tagaagaagc aatgtattta actaaatttg ctagaaaagt
    40441 aacaattgta catagaagac aaggatttag atgtgctaag agtgttgaag aaaaagcaaa
    40501 agctaatcca aagatagagt ttttattaga tacagtaatt gaagaaatca aaggtgatgg
    40561 aatattagaa tcagttgtat ttaaaaataa agttactgga gaaactcatg aatactttgc
    40621 agatgaagaa gatggaacaa tgggagtatt tgtgttcgtt ggattagatg ctcaaactga
    40681 tttattcaaa ggtaaagttg atatggatga aaaaggttac ataataactg atgaagatat
    40741 gagaactaac ataccaggcg tatttgcagc aggagattgt agatctaaga ctctaagaca
    40801 ggttgtaact gcaactaatg atggagcaat tgcatctata gtagctgaga aatacattga
    40861 tgaaaaattt gggaactaga atctgtaaat taagaaatag tgtctgtaaa tttaatgaat
    40921 aatatttaaa attaaaatta atatttaaaa taaaaataaa aatttcaagg gggaataaga
    40981 aaatgttaga tttagataag gctacatttg aagaagaagt tttaaatgca gaaggttttg
    41041 tatttgttga tttttggagt gaaggttgtg agccttgtaa agctttaatg ccagatgttc
    41101 ataaattagc tgagacttat ggggataaaa taaaattctg taagatggat acaacaaaag
    41161 ctagaagatt agctataaaa caaaaagtat taggtcttcc aactatggct atatacaaag
    41221 atggagaaaa agtagacgaa gttactaagg atgatgctac agttcctaat atagaaaaca
    41281 tgattaaaaa atatctttaa tatgttgata aattattaac attaaaagaa aaaatctatt
    41341 tttatctgaa gataagaaga aaaaggtttt tatgtccaac tttgttaaaa aaataatttt
    41401 aaataaaata atttttttac aataaatatt ttttagtaat aaaataaaga tttaaggcat
    41461 aaaaacacaa taatatatct ttaaaaaaat aagataaatt gtaaaatgat ataattttga
    41521 gaaaaactca aatgcatata cacaaattaa ataacaggag gtgcaagtat gcgtcttgaa
    41581 ttagggaaaa tctttataaa agatattcaa tttggtgatg taacagaagt aaaagatgga
    41641 gtattattca tcaataagca agagatgcta caagaaattg gtggagatga gcatattaaa
    41701 tctatagata tagaattagc tcgtccaggt gaaagcgtaa gaataacacc tgtaaaagat
    41761 gttattgagc caagagtaaa agttgaaggt aatgggggaa tcttccctgg tatcatgtct
    41821 aaagttgaca ctgtaggaga aggaaaaact catgctttaa aaggtgtagc tgttgtaact
    41881 actggaaaaa tagtaggttt ccaagaaggt ataatagaca tgactggaga aggagctaag
    41941 tacactccat tttctaaatt aaataatgta gtagtaatag ctgaacctat tgatggttta
    42001 aaacaatacg ctcatgaaaa agctgttaga atgataggat tcaaagcagc aatgtactta
    42061 ggagaagtag caagaaatct tactccagat gaagtttcag tatatgaaac taaacctctt
    42121 cttgaatcaa taaaagaata tccagagctt ccaaaagtag gatatgttta catgcttcag
    42181 actcaaggtt tattacatga tacttatgta tatggagtag atgcaaaaca aatagtacca
    42241 actttattat atccaacaga attaatggac ggagcaattg taagtggtaa ctgtgtttct
    42301 gcatgtgaca aaaacccaag ttatgttcat ataaacaatg gggttgttga agacttatat
    42361 gcaagacatg gtaaagatat aaactttgta ggtgtaatta taactaatga aaatgtttat
    42421 ctagcagata aagaaagatc ttcaaactgg acttctaaat tatgtaaata tttaggatta
    42481 gatgcagtta tagtttcaca agaaggtttt ggaaatcctg atactgacct tataatgaac
    42541 tgtaagaaga tagaaatgca aggtgttaaa acagttatag ttacagatga atatgctgga
    42601 cgtgatggag gttctcaatc acttgcagat gcagatgtaa gagctgatgc tgttgtaaca
    42661 ggtggaaatg ccaaccaagt tgtagttctt cctaagttag ataaagttat tggacattta
    42721 gaagtagtag atgtaatagc tggtggttct gatggaagtt taagagctga tggaactatt
    42781 gaagttgaaa tccaagctat aacaggtgca actaatgaaa caggatttgg tcatttaact
    42841 gctaaaggat attaagaagt attttattaa acgattgagt aatgttaata aaacatatta
    42901 tagataatta taataagtgt tatagatata taaaaatata ttagaaatat aataaggagg
    42961 attattatga gtttacttag taataaaaag gttcttataa taggtgaccg tgatggtata
    43021 ccaggacctg cgatagaaga atgtgtaaaa acagtagaag gagcagaggt tgttttctca
    43081 tctacagaat gctttgtctg aacagctgct ggggctatgg acttagaaaa tcaaaacaga
    43141 gttaaagatg ctgctgataa attcggagct gaaaatgttg tgattttact aggtgctgct
    43201 gaagccgaag ctgcaggtct tgcagccgaa acagtaactg ctggagatcc aactttcgct
    43261 ggaccacttg ctggagttgc cttaggatta agtgtttacc acgttgttga ggaaccaata
    43321 aaatcattat ttgatgaaag tgtatatgag gaccaaataa gtatgatgga aatggtttta
    43381 gaagttgaag aaatagaaga agaaatgtct ggtataagag aagaattttg taaattttaa
    43441 tgcctaaaag caaaaggggt gaaactaaga gatgggaaaa ctaaaagcag tacattatat
    43501 taatcagttc tttgctggta taggtggaga agaaaaagct gatactaagc cacatgtggc
    43561 agaaacttta cctccaataa gtttacaact tgataaatta ttaggtgaag atattgaaat
    43621 agttggaaca gtagtttgtg gagatagcta tttcaatgaa aatatagata gtgcaagtga
    43681 agaagtttta tcaatggtaa aaggttttga accacaactt ttcatagctg gtcctgcatt
    43741 taatgctggt agatatgggg ttgctgctgg aacaataact aaagttgtaa aagatgcttt
    43801 gaatatacca gctttaacag gtatgtatgt tgaaaatcca ggagcagata tgttcaaaaa
    43861 ggatgtttat gtagtagaaa cttctgattc tgcagcaggt atgagaaagg cacttcctaa
    43921 gatagctaaa cttgcagtaa aacttgctaa tggagaagaa attggaactc ctaaagatga
    43981 aggatacata gctagaggaa taagagttaa ctacttccat gaagacagag gttctaagag
    44041 agctgttgac atgttagtta agaaaataaa aggtgaacca tttgaaactg aatacccaat
    44101 gccaaacttt gatagagttg acccaagtaa agctgtaaaa gatttatcaa aatgtaaaat
    44161 agctttagtt acttctggtg gtatagttcc aaaaggaaat ccagatagaa tagagtcttc
    44221 ttcagcttct aaatatggaa cttattctat agcaggagtt atggatttaa cagaagagac
    44281 ttatgaaact gcacatggtg gatatgaccc agtgtatgca aatttagatg cagatagagt
    44341 attacctgtt gatgttctta gagatttaga aaaagaaggt gtaattggaa aattacatga
    44401 gactttctat acaactgtag gtaatggtac atctgttgca aactctaaaa aatatgcttc
    44461 tgaaattgga gcagcattag tggctgatgg tgtagatgca gtaatattaa cttctacatg
    44521 aggtacttgt actcgttgcg gtgcaacgat ggtaaaagaa atagaaaaaa ctggacttcc
    44581 agtagttcat atgtgtacag tagtacctat ttcattaaca gtaggggcta atagaatagt
    44641 tccaactata gcaatacctc atccacttgg aaatccagcg cttgacccta cagaagaaaa
    44701 agccctaaga agaggccttg tagaaaaagc attaaatgct ttaactacag aagtagatgg
    44761 tcaaacagta tttgaaaaat aaaaataaca cacacatata ttatatagat aaaaatataa
    44821 acacacacta aaaataaaaa ataactatac acaaaaaaat aatatagatt tcatgaatgg
    44881 ataaattgga gttattcatt catgaaatct taaaaaatgc caaaatacat aaacaataat
    44941 agaatcaaat atacaataaa atacaatgat tagagctttt aataagttta ataagattaa
    45001 aaaataagtg ttcataaaat ttatggaggt gcacaaaatg acttatccag ttttaaaagg
    45061 ggcaggctat gtacttattc atactccaga tatgatagtg caaaatggaa gtacttgtac
    45121 agttgaaaga gcaacaaatc cagattcaga atttttaaaa gaagtaagta atcatataag
    45181 aagttatgaa gatgtagtta actacatgcc aaatcaagtt tatataggaa atagaagacc
    45241 agaagaatta agagatttac caatgccatg gtgtgaacaa aagattgagg gaactagaaa
    45301 tggtaagttt ggagaaataa tgcctcaaga tgagttcata gctttaatgc aaataagtga
    45361 tgcatttgat ttagtaaaat tatcacaaga atttattgat gaagtaaagc caaaaatcga
    45421 aaataactac cctgaaattg caccatttgt aggtaaatta aagggtgatg atatagaaga
    45481 aggtaaagaa ttagtagcaa cacacatagc tgaaggatta taccatgatg gaaaatttgt
    45541 tggttatgta aaaagagcac atgatgtaga tgttaactta aatgcccata caatgtttga
    45601 aaatctagta gtaaaagcat ctggagtttt atctgctatt caaatgctaa gacatagcaa
    45661 aatagaccct gctgaaatag attatgttat tgagtgttca gaagaagctt gtggagatat
    45721 aaaccaaaga ggtggaggaa actttgctaa atcaatagct gagatagcag gacttcaaaa
    45781 tgcaactggt tctgacacaa gaggattttg tgcagcacca actcatgcat taattcaagc
    45841 agcagcttta gtaaaagctg gtatacataa aaatgtaatg gtagtagcag gtggagcaag
    45901 tgctaaactt ggaatgaatg ctaaagacca tgttaaaaaa ggtcttcctg tacttgaaga
    45961 tgttgttgga ggatttgcag tacttgtatc tgaaaatgat ggtgtaaatc cagttataag
    46021 aactgattta acaggtaagc atactgttgg aactggttct tcaccacaag cagttatgac
    46081 agcacttata acttcaggtc ttgatagagc taatttaaaa ataacagatg ttgatgtgta
    46141 ttcagttgaa atgcaaaacc cagatataac taagccagca ggagcaggag atgttccaga
    46201 agcaaactac aaaatgatag gggcacttgc tgttaaacgt ggagacttag aaaagaaaga
    46261 attgaaagat tttgtttcta acaaaggatt accaggatgg gcaccaactc aaggacatat
    46321 accatcagga gctccttata taggattttt aatagatgat ttaactacag gaaatagaaa
    46381 tagagctatg atagttggaa aaggtagttt attcttagga agaatgacta acttatttga
    46441 tggagtatct tttatagctg aaagaaatac aggtgttact gaagagactt caggaatatc
    46501 taaggatgaa attaagaaga taatagctga atctatgaaa aaactagcat tagatatgtt
    46561 agaagaatag gggtgaggat atgtcaaaaa aagtaatcgc agatgtattc ttagaagtag
    46621 ctaatgcaat agaaagcggc gaatttggca aaaaagtaaa aattggtgtt actacacttg
    46681 gaagtgaaca tggtgtagaa aacatggtta atggtgcaca attagcaaaa tctaatttat
    46741 ttgatattgt gcttattgga ccaaaagtgg aaactgacct tgaagtagtt gaagttaatg
    46801 atgaaaaaga aatgcatgca aaaatggaag aattattaga ttcaggatat attgatgctt
    46861 gtgttactat gcattataat tttccaatag gtgtatctac agtaggtaga gtaataactc
    46921 cagcaaaagg aaaagaaatg atacttgcaa caacaacagg aacaagtgct actaatagaa
    46981 tagaggcaat ggtaagaaat gccatttatg gtattgctac agctaaatca atgggtaaca
    47041 aatgcccaaa agttggaata cttaatgttg atggagcaag acaagttgag aagtgcttaa
    47101 aagaattaaa agataatgga tatgacatgg agtttgcgga ttcaattaga gcagatggtg
    47161 gatgtgtaat gagaggtaat gatttacttg taggtgcacc tgatgttatg gttactgata
    47221 cattatctgg aaatatattt atgaaagtat tttcttcata tactacaggt ggagattatg
    47281 aagctcaagg ctttggatac ggacctggag ttggagagga ctatgataga aaggtactta
    47341 tagtatcaag agcatcaggt tctccggtag tagcaaatgc tttaaaatac gcttatgatg
    47401 ttgtaaaggg agatataagt aatgttgcaa gaaatgaatt tgcaaaggtt aaaaaagcaa
    47461 aatttgatga cattatatcc tctcttacta aaaaagaagt taaagctgaa aaagtagaag
    47521 ttaaaatgcc agataaagaa attgttactc gtcaaatagc tggtgttgat ataatggatt
    47581 tagaagatgc agtatctgag ttatggaaaa acggaatcta tgctgaaagt ggtatgggtt
    47641 gtacaggtcc aatagtttta gtaaatgaag ctaaaggaga cttagcagtt gaaactttag
    47701 ttaaagctgg atatactgca aaataagtac attttttata tgtaaataag aacatatagt
    47761 tataatttta ttgattataa ttatatgttc tttttatttt atatcttgtc aatatttata
    47821 tattaacaca atccatttat ttgattctct agctaattta ttatatttat tcagttaaaa
    47881 ttaagctttc taactaaata aaaactaatg aagtattaaa atgaaattga taaaatatga
    47941 aaaaatttta ggaataaagt atctgttaga taaaaataag attaattagt gatataatat
    48001 aattaagata aaatttatta tttggaggca aaatcatgag cagagtaaaa aatgtagtag
    48061 aattgttgga aactaaaggt gttgatgcac tatatctaac aaagaaaact aatgtaaact
    48121 atataagtgg ttttcctgat gaagaagcat atgcagtgat ttgcaaagat ggaaactttt
    48181 tagttactga tagtagatat atggagcttg cagaaaaggt ttgcaaagat tttgaaatta
    48241 taaattggca taattttgat agaagtgtgg caaaggcagt taaaagtgta tgtgataaag
    48301 tgggtataaa gaaacttggg tttgaaagaa ctaatatagt gtttgataaa tatgaagaac
    48361 ttaaaaatct gattgagaag gataatggag aattaatacc tacagaaaat attgtagaga
    48421 ctttgagata tgtaaaagac aaagatgaaa tcaaaaatac aagaaaagct tgtgaaatag
    48481 cggataaggc attagaagaa ttgattccac atataaaagc tggagttagc gaaattgaac
    48541 tggctacaaa gttagagtac tttatgaaaa tgaacggagc acaaaatata gggtttgaaa
    48601 ctatactgat atcaggagca aagacttcat tgcttcatgg taagcctagt gataagatta
    48661 tagaaaaagg tgattttgta cttatagact atggcgctat gtataatggc tatatatctg
    48721 atacaactag aacttttata gttggaggag cttctgaaaa gcaattagaa atatataatt
    48781 tagttaagga agctcaaaat gtaggggttg agaacatgaa agctggtgta catgcgacaa
    48841 ttccagatgc tgagataaga aaagttgtca aaaaatatga agactattac tatcaaggta
    48901 taggacatgg agtcggtaga gatgttcatg aagaaccttt cattggtaat tatggtgata
    48961 aaatcataga agagggatgt ataataacaa tggaaccagg tatttatttc cctggatggg
    49021 gtggagttag aatagaagat actgttttga ttactaaaaa tggtccagaa agacttacta
    49081 aattccctaa agatttgatg attttagaca aataatttaa tttatatata tttttttaca
    49141 taaaatataa cataatctca ctattaagct ctatttacat attatagtaa agagggctta
    49201 attttattg
//
LOCUS       DAYAOY010000020        48431 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYAOY010000020 DAYAOY010000000
VERSION     DAYAOY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.020"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(95..1549)
                     /locus_tag="ACGI6B_001218"
     CDS             complement(95..1549)
                     /locus_tag="ACGI6B_001218"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFG9750672.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            1713..2582
                     /locus_tag="ACGI6B_001219"
     CDS             1713..2582
                     /locus_tag="ACGI6B_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9750673.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(2795..4867)
                     /gene="ptsG"
                     /locus_tag="ACGI6B_001220"
     CDS             complement(2795..4867)
                     /gene="ptsG"
                     /locus_tag="ACGI6B_001220"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFG9750674.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(5170..6012)
                     /gene="licT"
                     /locus_tag="ACGI6B_001221"
     CDS             complement(5170..6012)
                     /gene="licT"
                     /locus_tag="ACGI6B_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9750675.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(6273..8315)
                     /locus_tag="ACGI6B_001222"
     CDS             complement(6273..8315)
                     /locus_tag="ACGI6B_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9750676.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(8467..9438)
                     /locus_tag="ACGI6B_001223"
     CDS             complement(8467..9438)
                     /locus_tag="ACGI6B_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750677.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(9439..10416)
                     /locus_tag="ACGI6B_001224"
     CDS             complement(9439..10416)
                     /locus_tag="ACGI6B_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750678.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            10635..12194
                     /locus_tag="ACGI6B_001225"
     CDS             10635..12194
                     /locus_tag="ACGI6B_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9750679.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            12300..13259
                     /locus_tag="ACGI6B_001226"
     CDS             12300..13259
                     /locus_tag="ACGI6B_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750680.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            13262..14074
                     /locus_tag="ACGI6B_001227"
     CDS             13262..14074
                     /locus_tag="ACGI6B_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750681.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(14290..15192)
                     /locus_tag="ACGI6B_001228"
     CDS             complement(14290..15192)
                     /locus_tag="ACGI6B_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9750682.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            15547..16725
                     /locus_tag="ACGI6B_001229"
     CDS             15547..16725
                     /locus_tag="ACGI6B_001229"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFG9750683.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            16734..17390
                     /locus_tag="ACGI6B_001230"
     CDS             16734..17390
                     /locus_tag="ACGI6B_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFG9750684.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            17390..18055
                     /locus_tag="ACGI6B_001231"
     CDS             17390..18055
                     /locus_tag="ACGI6B_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFG9750685.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            18160..18936
                     /locus_tag="ACGI6B_001232"
     CDS             18160..18936
                     /locus_tag="ACGI6B_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFG9750686.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            18998..20332
                     /locus_tag="ACGI6B_001233"
     CDS             18998..20332
                     /locus_tag="ACGI6B_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9750687.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            20828..21760
                     /locus_tag="ACGI6B_001234"
     CDS             20828..21760
                     /locus_tag="ACGI6B_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFG9750688.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(22152..25691)
                     /gene="nifJ"
                     /locus_tag="ACGI6B_001235"
     CDS             complement(22152..25691)
                     /gene="nifJ"
                     /locus_tag="ACGI6B_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFG9750689.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(26209..26778)
                     /gene="pduL"
                     /locus_tag="ACGI6B_001236"
     CDS             complement(26209..26778)
                     /gene="pduL"
                     /locus_tag="ACGI6B_001236"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFG9750690.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(26850..27458)
                     /locus_tag="ACGI6B_001237"
     CDS             complement(26850..27458)
                     /locus_tag="ACGI6B_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9750691.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(27466..29229)
                     /locus_tag="ACGI6B_001238"
     CDS             complement(27466..29229)
                     /locus_tag="ACGI6B_001238"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HFG9750692.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(29272..29733)
                     /locus_tag="ACGI6B_001239"
     CDS             complement(29272..29733)
                     /locus_tag="ACGI6B_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750693.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            29923..30384
                     /locus_tag="ACGI6B_001240"
     CDS             29923..30384
                     /locus_tag="ACGI6B_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750694.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            complement(30477..30647)
                     /locus_tag="ACGI6B_001241"
     CDS             complement(30477..30647)
                     /locus_tag="ACGI6B_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750695.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(30836..31057)
                     /locus_tag="ACGI6B_001242"
     CDS             complement(30836..31057)
                     /locus_tag="ACGI6B_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9750696.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(31141..32367)
                     /locus_tag="ACGI6B_001243"
     CDS             complement(31141..32367)
                     /locus_tag="ACGI6B_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9750697.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(32429..33361)
                     /locus_tag="ACGI6B_001244"
     CDS             complement(32429..33361)
                     /locus_tag="ACGI6B_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFG9750698.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(33450..33977)
                     /gene="hpt"
                     /locus_tag="ACGI6B_001245"
     CDS             complement(33450..33977)
                     /gene="hpt"
                     /locus_tag="ACGI6B_001245"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFG9750699.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            34206..35282
                     /locus_tag="ACGI6B_001246"
     CDS             34206..35282
                     /locus_tag="ACGI6B_001246"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9750700.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(35368..36576)
                     /locus_tag="ACGI6B_001247"
     CDS             complement(35368..36576)
                     /locus_tag="ACGI6B_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFG9750701.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(36737..37126)
                     /locus_tag="ACGI6B_001248"
     CDS             complement(36737..37126)
                     /locus_tag="ACGI6B_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HFG9750702.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(37230..38231)
                     /gene="asnA"
                     /locus_tag="ACGI6B_001249"
     CDS             complement(37230..38231)
                     /gene="asnA"
                     /locus_tag="ACGI6B_001249"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFG9750703.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(38290..38545)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(38679..39869)
                     /locus_tag="ACGI6B_001250"
     CDS             complement(38679..39869)
                     /locus_tag="ACGI6B_001250"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9750704.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(40150..41271)
                     /locus_tag="ACGI6B_001251"
     CDS             complement(40150..41271)
                     /locus_tag="ACGI6B_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HFG9750705.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(41332..41817)
                     /locus_tag="ACGI6B_001252"
     CDS             complement(41332..41817)
                     /locus_tag="ACGI6B_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFG9750706.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(41819..42487)
                     /locus_tag="ACGI6B_001253"
     CDS             complement(41819..42487)
                     /locus_tag="ACGI6B_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFG9750707.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            42877..44901
                     /locus_tag="ACGI6B_001254"
     CDS             42877..44901
                     /locus_tag="ACGI6B_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750708.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(45131..46318)
                     /locus_tag="ACGI6B_001255"
     CDS             complement(45131..46318)
                     /locus_tag="ACGI6B_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HFG9750709.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(46520..47791)
                     /gene="brnQ"
                     /locus_tag="ACGI6B_001256"
     CDS             complement(46520..47791)
                     /gene="brnQ"
                     /locus_tag="ACGI6B_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9750710.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(47932..>48431)
                     /locus_tag="ACGI6B_001257"
     CDS             complement(47932..>48431)
                     /locus_tag="ACGI6B_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9750711.1"
                     /translation="VSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIY
                     RVEFKNSKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVK
                     GDNLVFEYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLM
                     GIIIK"
BASE COUNT        16646 a         7975 c         5877 g        17933 t
ORIGIN      
        1 atctatttta caattattta catataaaaa aatgtaatat caaagtgaag cttgatatta
       61 catttttgtt cttgtatttt gtttaatttt tatgctacat cttacttaaa cactcatttg
      121 caacttcttt atcatcaaaa tgatgcttaa ctttacctat aatttgataa gtttcatgtc
      181 ccttacctgt tataattaca acatcatcct tttttgccat acttatagct tctgctatag
      241 cttgttctct atcaacaaca acatggtaat tttctttttt cttgtcaatt ccttcaagta
      301 tatcttttat tattgcctct ggttcttcag ttcttggatt atcagaagtt ataatacata
      361 aatcagaata tttttgtcca attgctccca tcaaaggtct cttttcagta tctctatctc
      421 caccacaccc aaacacagaa attatttttc cttctgcaaa ttgttgagtt gtctttagta
      481 cattttcaag tgcgtcagga gtatgagcat agtctacaat tacgcttata cctttatcat
      541 ttggaactgt ttcaaacctt cctgatactc cattagaaag tctaaggcct tctttatata
      601 ttggtttagg tatgccaagt acatagcaag cagctataac tgctaaagta ttatatactg
      661 taaacatccc tggaactggt ataaatatta cttcttcata tgaaggtgtt attaatctat
      721 aagatactcc atccgaatct gattttatat ctctagccat aaaatcagcc ttagtatcaa
      781 caccataagt gtaacaaggt acatttatac cttttatatt ttcatatatc ttttttccac
      841 cttcatcatc tatgtttatt atatttgcca tagtagtctt aaaaaataat ttttcttttg
      901 cttttctata atcctctaaa tctttatgaa aatctaaatg gtctggtgtt aaatttgtaa
      961 atatgcctag tttaaagtct gtttcatcaa ctctatttaa tgccaatgaa tgtgatgaaa
     1021 cttccattgc acagtagtca catccattat ccaacatatt attaaaatga tattgtaaat
     1081 ctatactttc tggagttgtt gaattggaaa caatgtcttt ttctccatca aaaattttta
     1141 tagttcctat aagtccaact ttattcttat ttaaagttaa tatctcattt agtatagtag
     1201 ttatactagt ttttccatta gtacctgtaa ctcctataac gttaaatttt tgagatggat
     1261 gattgtaaaa attatcagct acttttgcca tatcttttct tgtatctttt acctttataa
     1321 atgtacatcc ttttattgct acatcttctt caacaagaaa tgcacttgct cccttttcta
     1381 tggcatcttt tatatactta tgaccatctg acacaaatcc ttttatacat ataaataatg
     1441 taccttttgt aacctttctt gaatcatatt gtacattatt aatatctata tttaattcac
     1501 ccttgacatt gattatatcc aacccctgta taatatcatt taatttcata aatgctttcc
     1561 tccctttaaa ctctttctgc taaaattaca ctacatataa tattatatta agaatttttt
     1621 actatgtgaa tggttaaaaa tgcttgaaac attgcaaaaa atgctctata attttctata
     1681 atataactga ggaaaggtct tctataaaaa ctatggataa taaaatcggg tacttaaacg
     1741 ataactttaa aatatttaat attagagata aaaaagatat acaatttgaa taccataatc
     1801 atgattttaa taagataatt atatttataa atggaaatgt aacttatttt gttgatggta
     1861 ttccctataa actaaagcct tgggatatac tctttatcag caataatgaa atccataaac
     1921 cagttataga ttcaaatgtc ttttatgaga gaattgcaat atggattagc ccagaattta
     1981 tgaaaaaaaa tagtgatgct agtagtgact tagaaaaatg ttttaaaata gctacagaga
     2041 ataaatgcaa tagattgcgt ttaaatatgc atgatattga atctattaag agttttattg
     2101 agcaaataaa tatttccgaa tccaatactg aatttggaaa tgaaatattg agaaatacat
     2161 tatttataca acttatgata ttcttaaaca gaattttcct aaaaaatgaa aatatggaat
     2221 caacagaaga tgttttttat gatgaaacta ttaagaaagt attaaactat ataaattcaa
     2281 atatagatga taagctttca atagatagca tatcgtctga attttttata agtaagtatt
     2341 atcttatgag aaaatttaaa tctcatactg gaacgtctat acacaattat atagttcaaa
     2401 aaagattaat tttggcaaag tcactaataa ccaatggtca ccttatgagt gaagtttgta
     2461 ataaatgtgg attcaacgat tattctagtt ttgtaagggc atttaaaaaa aactacggtg
     2521 tatctcctaa aaattatata aacaataaaa atcctttaga tattactctt ttagatgaat
     2581 agataattta tgtaaatttt attttaataa aacttgaagt attagtgtat ttatcttact
     2641 aatacctcaa gttttttaat gttttaaaat atatcaaaat ataatgatat atatttctat
     2701 tacttaaaat atatattaaa taaattgcat gttaactgaa cctatttata tatttattaa
     2761 atctcacata acttttagtt acactatcat atagctatcc tattttttca atatcatctc
     2821 ttaatatact tactctattc tcctcttttg ctgtggttac tcctttttta atgcttaatt
     2881 tctgattttg ttctaaattc gtaaatacta caggacttat tgttgataga ccattttttt
     2941 caatatattc taagtccatt ttaactaatt tttctcccgc ttttacttca tctcctacat
     3001 tcacaaatac ttcaaatcca tttccttcta agtttacagt ttctatccct aaatgtataa
     3061 gtatttctac tccacttttt tgggatttta ttattattgc atgcttagtt ggaaatataa
     3121 ctccaatagt tccatcaact ggtgaataaa tcaatccatc actagattta attgcaaatc
     3181 catcccccat cagtttactt gaaaatacag aatctggcac ttcttctata tctaataact
     3241 ctccatttgt taatgaatca atcttttcta ctaaagtatt ttccatattt gcaacttcca
     3301 tatttttttt cactttattt ttatcaacat ttttagtaac agtactttca acctttgttt
     3361 ttccctctat tatatctttt atatcagact ttattgaatc tgattttggt ccaaatatag
     3421 cttgaacatt gttgccaata accataacac ctgctgctcc aagtgatttc aatttttctt
     3481 tatctacttt atttatatca tttacaacca atctaagtct tgttatacac gcatctagac
     3541 tctttaagtt gtctttattt cctaaagcaa tcaaaatctc tgatgctaat tcattcttag
     3601 acatttcaat gacttcagcg ttagtttctg actcatcttc tcttcctggt gtctttaaat
     3661 taaattttct tatcataaat ctaaatacac tatagtataa tactgcaaaa ccaataccta
     3721 ccaagatagc caagtaccat ggagttctat taggaagaac accaaataat aagaaatcta
     3781 ttaatcctcc tgaaaatgtg aatccaattt taactcctaa taattgcata attgcgtatg
     3841 ataaacctga aaataaacaa tgtattgcaa ataatactgg tgcaacaaat aaaaacgcaa
     3901 actctattgg ctcggttatt cctgttaaaa aagaggttaa tgctgctgat gccattatac
     3961 ctgctacaaa ttttttcttt tctggctttg cttcatgcac catagcaaga gctgctgctg
     4021 gaagaccaaa catcataaat ggaaatgcac ctgtcataaa agtacctgct gtgaaattta
     4081 ctccatcttt aagttgagca aagaatatct tttggtcacc cattacaagc tcaccagctt
     4141 tatttacata ttctccaaat tgataccaaa atggattatg gaaaacatga tgcaaaccaa
     4201 atggtattaa tagtctttcc aaagaacctt ccataaatgc tgctagtgtt tgatttgctt
     4261 ctataacatt ttttgagaaa ctaagtaatc cctcttgtat tggaggccaa ataaaagaca
     4321 atataattcc tattgctata gcaactaatg aagtaacgat tggtacaaat cttttacctg
     4381 caaaaaaacc tagatactga ggtaattcaa ttttataaaa ctttttataa agataagcag
     4441 ccactatccc tataataata ccaccaaata cccctgtttg aattgttggt attccaacaa
     4501 tatttgcata catagaattg tctaccatat cttctgtaac acctgccata acacccatag
     4561 atgtattgag aattaaaaaa cctactatgg ctgccaatgc tgcaacacct tctccttcag
     4621 ttaaaccaac agctacccca actgcaaata taagtggtaa gttattaaat ataatttgac
     4681 ctgaactttc cattatactt attataagtt gaaatgtagg attcgccaaa gctggaaaat
     4741 gagccaacaa tgcgtctcct gctaaagcat tactcacgcc aagtaatata cctgctgcag
     4801 gaagtaatgc aactggtagc attagagatt ttcctacttt ttgtaaaaca ccaaatactt
     4861 ttttcattta cttttctctc cttattattt tatatttttt taaataaaaa agacttaagt
     4921 ttataaaaag ttacattagt atacttaatt ctaaaattaa acttatgtta atataaacaa
     4981 acctatatta acctaatttc agaattaaaa ataaactatt ataatttttt tataaacata
     5041 agtcttgcct gcattatcag taacaatctt aatgttttaa aattattatt ttatattaaa
     5101 ttttaaataa tatattaaaa ttaaacataa tatattaaat ctatacattc tgtattaaac
     5161 tactctttat tataaattca gtacagatat aactcttttg atatgtaatg ttaaataaag
     5221 tagttcatct tgacttacat agtaatcata attcttttct acatgagttt ttattttata
     5281 tgcacaatca taatcctttt catactttac tttaattata tctataattt cattatttgt
     5341 atcattgatt ttttcttttt ttactaatcg ttttgcaaag aattttaaat gagtcagaag
     5401 cctatcataa tttatatcat cttctttaaa ttcaactcta taataatatc ttataatatt
     5461 tagtatgtct tttacaattt tagtaattaa cagtgattcc tttgaacttc ccttatagtt
     5521 tgaatttact atatgcattg ctataaaacc agcttcatct actggaaatt tcacatcaaa
     5581 ttctttattt atataatcta cagcccattt cccaatctca tattcttttt tatgtattct
     5641 ttttatctca tgtagtaaat cattctttat agttatgttt tcatgatatc ttttaattgc
     5701 aaatgcaata tggtcagtaa gtgctacata aatatattca taaagtttag tattcaaatt
     5761 ctcctctgca taagcaatta tttcagtact aacctcaaaa aatttctcat caacctggct
     5821 tgctagttta ctaattttat ctaaaaagct ttcatcttgt attacaaaag ttctttcaat
     5881 tttactttta tctatttctt gacctttctt tttttgaaac ccaattccac atcctgttaa
     5941 tattaattct ttttttatat tttcatctaa aactaataca acattattat tataaatttg
     6001 ttgaataatc ataatcaata tctctcccaa aatacaattt cccttattca taaaaaaata
     6061 cctaaattaa gatacaattc acctgtatac cttaacctag gtattgcctg cttaccagta
     6121 acaatccagc ttgttaacgc ttatatattg tattcaattt atggttttat tctattataa
     6181 ataaatatat atgtcaagta aattattgaa aactatttcc tattttttag tattaatttt
     6241 aacatcaaaa atcaaatttt attccattat atttatgatt ctatcatcat catttcatca
     6301 tagagaatat tttccaataa ctctttagcc acttctcttg atatttgctt agtttcaact
     6361 aattgttgaa cactgtctct ttgactttgt atcgccatta ctctgtagta ttctactctt
     6421 gcctcaaact tatcatcatt ttgaatcttt ctagtaatcc aattcaaata acgattatag
     6481 aatctgattg ctattaaggc ctcatgtctg ttatcttcat cagtattttt atttataaat
     6541 gatatagcag ttttagtaga aagtgcatat gcatggtgaa actctttgat tatttcttta
     6601 ttctcagata ctatttttgt aattttttct tttacttctt tctttacttt tctatttata
     6661 atccacatgc ttatttttaa ttttacaatt ttaaatatag atttttgaac agattttctt
     6721 gattttgtaa tataaaatct atacaattta aaggctctat cagaaatttt accatcttct
     6781 aatagtttca aaacagctaa taatccctgt tggtctgctt ctgcaatcat catacttata
     6841 agtttattat ctggagtatt tagtattcct ttttctgagt ctaccaactg ttcatttaaa
     6901 tcatgtataa ctggttgtac ttcttgtgct ttttcattac taagttttaa aatggcttct
     6961 ttcaaaatta gtttatatgc ctctttatca gataataaag gttctgactc ttgtacgtta
     7021 ttttttaata atcttggcag taaaactgtt gccaatacta aacttataag aataacttct
     7081 gatgatataa ataaaacagt atctctcatt ggaaatacat ctttatcagc caaataaaaa
     7141 ggtatagata aggcagttgc tagtgtaaat gtaccatgaa caccacaagt agcaactatt
     7201 agtgagtatt tacatttaga aatactctct tttacaactt cttcatcctt aaaacctaaa
     7261 aaacctacaa aaaaattatt taaaggattc tttttaggtt ttataaaaga attatgccat
     7321 aaatataccc atataaatct tataacaaat aaaataagta caattaaagt agaaataaaa
     7381 gtcaattcca atgccatatt ttcattttta ctactaagtc ctgtatatat acttggtaac
     7441 aaaaaaccaa gcagtgtaaa gacaaatcca ttcaatacat actccaaaac ataccaagta
     7501 ttgtctgata ttatccttaa tttagtagtt gtattttgta aatgctcttt ttcaattcca
     7561 tgagctatcc ctgccattac aacagctagg attccagata ctcctatttc ttctgctaca
     7621 aaatatacaa aaagtggagt cattatttgt ataactatta ccattggaat ttcttccaaa
     7681 ttccacttgt gtaaactaag tcttattttt ataattatgt atcctaaaat acttcctact
     7741 attattcctc caaaagctgt aatcataaat tgaatccctg cctcttctat tgaaaaaact
     7801 cctgtaactg tggcaagtac tgcaacctta aatgctacta ctccagcagc atcatttaat
     7861 aaagattcac cttcaagtat actcatcata tttttaggaa aatttaatcc ttgtgtaatt
     7921 gattttactg ctactaaatc agttggagat attactgctg ccaaagcaaa tgaaacagat
     7981 aatggcatcc caggaataag aaaatgtata aagattccac ctgcaaacac ggttattaaa
     8041 actaaaccaa atgcaagaag caaaattggt tttctgagct ctaatagctc ttttctcgat
     8101 acattttgtc cttcactgaa tagtaatgga gctatgatac atattacaaa tatttctgga
     8161 ttaaatgaaa atgagaattt aaatggcaaa atagacaata taatccctag tacaatttga
     8221 tatattgcaa gaggaatatt actccacttt ttgctaaata cagttgaaat cgttgtacaa
     8281 accaataatc ccaacaatgt tactaaaatt gacataacta cctctctttt caataataat
     8341 attatagatt aaacttatat atcaactaac ttaatattaa cagcatgaaa tcccatctta
     8401 atcaaaaaaa ttttcagttt attctattat tcttattata tacatcaaat ttaatttttg
     8461 aaatatctat actagatgac atgcaacaaa atgatttctt gatatttctt ttaaaactgg
     8521 aatctctttt ttgcatattt ccttacaata agggcatctt gtatgaaact tacatccact
     8581 tggtggattt gttggagatg gtatgtctcc ttttaatata attctatttt tcttaattgt
     8641 agggtcttgt attggtactg cgctcataag cgcttttgta tatggatgtt gagaatgtaa
     8701 gtaaagttct cctttagttg caatttctat tatagaacct agatacataa cacaaactct
     8761 atgtgcaata tgatttacta cactcaaatc atgagatata aataaatatg aaagtttata
     8821 tttatcctgt aaatccataa gtaaatttat aatttgagat tgaatagaaa catctagtgc
     8881 agaaacaggt tcatcacaaa ctataaattc tggatttaat gctaatgctc ttgctatacc
     8941 tattctctgt ctttgacctc cagaaaactc acctggtttt ctatttaaat ggtacttaga
     9001 aagaccacaa ctttctacaa catttaatat attatctttt atttcattct ttggcactaa
     9061 attatgttct ataactgcct cggatattat ttgttccaca tttaatcttg gatttaatga
     9121 actatatggg tcttgaaata tcatttgcat tttgggtctt aactttctaa gttcttcctt
     9181 ctctaactta tatatatcta caccattaaa ttcaacttta ccttcagttt tttcaattaa
     9241 tctaagaatg gttcttcctg cagttgattt tccacatccc gattctccaa ctatagccaa
     9301 agtttctcct ttttttaaat caaaagaaat tccatctact gcctttacac atttaatttc
     9361 cttatcaaat aatccagatt taacttcaaa gtacttttta agaccttcta cttttaataa
     9421 actctcttct tttttcaatt atatctcctc ctttttatat aaccaacatg caatactatg
     9481 accactattc aattctatta aaggaggtat ctgagttcta catttatcac atactttttc
     9541 acatctatca ctaaaataac atgaatctgg catcccaatt ggatttggta cttgtcctgg
     9601 aatcgaatac aatcttttat ctttgttttt tccaagagca ggctttgatt ttagaagtcc
     9661 aatagtataa ggatgttttg gatttttaaa taattcaaca acctctgcca cttctataat
     9721 tttgccagca tacatcacta atactttgtc acacatttct gcaactactc ccaaatcatg
     9781 tgtaatcatc attatagatg tgtttaattt ttcttttata tttttcatta tatctaaaat
     9841 ctgagcttga atagtaacat ctaaagctgt tgttggttca tcagctataa gtaattttgg
     9901 attacatgat aaagccatag ctatcattat tcgttgtctc attccacctg ataattcatg
     9961 tggatatgaa ttatatattt cttcagctct tggtatttct accagtttta tcatatcgat
    10021 tacctttttc ttagcttctt cctttgataa atcttgatgt atcataatac cttcaatgat
    10081 ttgacttcca atagtaaacg ctgggtttaa agatgtcatt ggttcttgaa aaatcattga
    10141 tatctcattc cctcttattc ccataagttc atcttcattc atctttaaaa tatctttgcc
    10201 tcgaaagatt atttctccac cttctatttt tgatgttgga agtaacttca tcaaagacat
    10261 ggctgttaca gacttaccac atcctgattc tccaactatt ccaatagttt caccttcatt
    10321 tatattaaaa cttacttcat ttacactctt tacaatccct ttatctgtat gaaaataaac
    10381 ttttaaatct ttaacctcaa ttagtgcttt actcattatt ttctcctctc agcaaaatta
    10441 cttaagataa catacacctt ttgattgtat tttataaaaa tttaaattac aacattttct
    10501 tacattttat tatttttttc tattaattat aataatataa tagttaattt ctatatatat
    10561 tgatttaata atgattacta ttatacaata aaaaatatgt gttaaatatt attttatgga
    10621 ggttttataa gaatatgaaa tttaaaaaat tggcatcatt aatcttagtt tcatcattaa
    10681 tgcttacatt tacagcctgt gcttccaatg ataaagacaa gtctagtgga agcaaatctg
    10741 gtggaactgt tgtaatacct atggcaagtg acccagatat aatcaatgga gcttttgcaa
    10801 acgttaaaga agactctctt gcatcaaata tagtttatgc tcctctttat acttatgaaa
    10861 aaggaaattt agtaaattat ttagcagaaa aagtagattt taaagatagt aaggaattaa
    10921 ctataaaatt aaaatccaat ttaaaatggc atgatggaaa gccaataact gctgaagatg
    10981 tcttatttac atttaataca gttttagacg aaaaacaaaa ttcaccatca agacaatact
    11041 tattggtggg tgagaaacca gttaaagtag aaaaaattga tgatttaaca gttaagatta
    11101 ctttaccaac tgcaagtgaa tctttcttat atggtatatc taaaatttct ccaataccaa
    11161 aacatgtgtt tgaaggagaa tcaaatatag ctaagtcaga aaaaaataat aatccagttg
    11221 gttctggtgc attcaagttt aaggaatgga aaaaaggtga aagcatagta tttgaaaaga
    11281 atgcagatta ttttggtgga gaatctaagg cagattctat agcgctaaaa ataattccta
    11341 atgaagcatc tcaagaagca gctttaaata atggcgaaat ttcacttatg aagacatctg
    11401 ctgaaggata tgaaaaggca aaatcaaact caaatttaca aacttatact tatagtgaag
    11461 aaagattaaa ctacattgta tttaaccaaa atatatcaaa catggctaac aaagaagtaa
    11521 gacaagcctt atcatatgcc ttaaatagaa atgaaatgat agaatcagca tatggaaaag
    11581 aaggttctgt acctgctaaa tctatattag ttccagaagc agacttctat acagaagaag
    11641 gtgttgaagg atatgaccaa gatacaaata aggctaaaga cttattggat aaaagtggtg
    11701 ttaaaattga taaattaaaa ataggttata acacaggtag atttgggcat aaaaactatg
    11761 ccttagtagc tcaacaagag ttgaaaaaaa ttggtataga agctgaaata gttccttatg
    11821 aaagtaaggc attcttcaat atactattta gtaatagcac tgaatgtgat atgtatgtaa
    11881 atggttatgc ttggggatta gaaccaaacc catatagagg tatgtttgaa actggtcaat
    11941 attgcaacca aactaaatat tctaatgctg aaatagatgc tttatgggaa aaaggattta
    12001 ctgagttaaa caaggaaaaa cgtgaagaaa tatataagca aatacaacag gatatatcta
    12061 aggatgcgcc tatttacact atagattatg aacaaaattt aatggcagct caaaaaaatc
    12121 taaaaggaat aaaagatgct aaaccatctc ctgcaatatt atttgaagat tggtctaaac
    12181 tgtatgtaga ataattgatg taaaatttta tattattaat tagaaaagga gatgtctaaa
    12241 aataatattg ttttattatt ttaaaacgag acatctcttt taattaggag aatttactta
    12301 tgttaaaatt aatattgaat agagtaaaag ttttaatacc tatgttaatc ttaatatcta
    12361 tattatcatt tggactttta gaattagctc ctggtgaccc agcagatgca tatataaatc
    12421 ctttaatgtc ttcacaggat attgaaaata taagggttaa tatggggctt gataaaccag
    12481 catatataag atatttaaac tggttgaaaa acactttaag tggaaactta ggtatttctt
    12541 atataaacca tatgcccgtt acagaacaaa ttatggaaaa aatgggaaat acatttattc
    12601 ttatgggtac ttctttgata ttttcaatat tggtagccat acctcttgga atatttttag
    12661 ctgtaaataa aaattcaata actagtaaag tatcatctgt ttttaattat ataggtgttt
    12721 ctataccttc tttttggata ggtatgatac tcatttcaat tttttcagtt aaacttaaca
    12781 ttttcccatc aggaggtatg catactattg gaaatgatag tattggagat ttagcaaaac
    12841 atttagtttt gcctgtaata actttaggtc tatataatac tgctattttt acaaattatg
    12901 tagaagctag tgtaaatgaa caattaaaaa aacaatatgt tattacagca agagcaaaag
    12961 gattatcaga aaaagtcata ctatttaaac atgtcttgaa aaattcactg acatcattgg
    13021 ttacaatcct aggcatgagt atacaaaaat tagtcactgg tgcatttgtt acagaagtag
    13081 ttttctcttg gccaggtatg ggaagattaa tgattgattc aattttttca agagattata
    13141 ctgtaattat ggcaataact atgttatcag cactattttt aattttaggt aatttagttg
    13201 ctgatatact atacttatta attgacccta aaataaaatc tagcaaagga ggattctagt
    13261 tgtgtttaca agtatcttaa aaaaattaaa aaaagataaa tcctttgctt ttgcatgtat
    13321 tactcttagc ataataatca tattaattat agcttctttt atattaaata tagactctaa
    13381 tgcaattgat actacttcta aacttcaaga acctagctta aagcatatct ttggtacaga
    13441 cgaacttggt agagattact tctctagagc tctttatggt ggaaggattt cacttagtgt
    13501 tggtatttta tcaatgctta tttctgtagt atttggtact gctgtaggtg ttattagtgg
    13561 atatattgga ggtaagctag accaattctt gatgagatta gtggatatgc ttatgagtat
    13621 accaagtttc ttagttttga ttgtattaaa tacttatcta acacctaaag tttccactat
    13681 tatactaatt attggattct taagttggat ggaagtttca agaattgtac gtggcgaaac
    13741 attaaaaata aaggaaaatg aatactgtct agctgctaaa gctactggta tcaaaactag
    13801 aaatatacta ttaagacata tagttttaaa cttaaaagag actattgtag ttgctggttc
    13861 gttaaatatt gctaatgcta tcttaactga atctgctctt agttttttag gtcttggaat
    13921 ccaattgcct cttgcatcat ggggtaatat gcttcaagat gctcaaagac atatttttga
    13981 caagttatat ttagctgtat ttccaggatt acttatattt ttaacagtca tgagtttgac
    14041 tataataagc aaaaaatttt ctagtaatac ttaacaaatt ttatatagaa aataaaaaca
    14101 gtgattatct aaagatatac actgttttta tttttcgttt aaaaaaatac atattttgta
    14161 tattataact ttaaatacta ctctaccatg ttatgcaaag cgtattattg ctctcaaaca
    14221 tatagatgaa aaactaatag tttattataa taaaaattga tttatttatt tgttatttta
    14281 ataacatttt taagagttct ttttactaaa gaaatctata ataaatttaa atacatgttg
    14341 gtctacatgg ctataacgtt cttttttagg tctacataat ataactttcc atgtaatagg
    14401 atgattaaaa ggcttttcta taattttgct attttcaaaa aaatcatgaa ctggtgatgg
    14461 taaaattgtt ataaaatcag aatttgtggt tgatagcaac aaaaaatccc atgaaccaga
    14521 cattatatat ctttttaact taacattttc atcagtaaat ttttgcatta gcttatgatg
    14581 tatcataaaa gtaggattaa atattgcaag taactgctca ttcaaatcgt tccaatctat
    14641 cttatccttc ttagcaagtg gatgatttat atttaaaaat gctgtaagtt catcttcttg
    14701 aagcacatgt tcagttacta cacttttatc aattttatgt ggatgtaaca atactgcata
    14761 atctaattcc tgtaagataa gcattctatt taagtcatat gcacccttct ctactatatc
    14821 aaattctata tctggattat ttgcaactaa ctgtgcaact acatctgaaa atgctattcc
    14881 aagtataagt ggaggtattc ctattctaac ttttccttta aattgaactg aatcttctct
    14941 taaatcttcc atcatatttt tgtattcatt tatgatatgt tctgcattta cataaaatct
    15001 ttctcctgct ggtgttaacc catttaacct gcctttatac ctttcaaata aatatacatc
    15061 ttcactttct tcaaaatttt ttataatttg acttaatgct ggttgagata tgtgaagtgt
    15121 cttagatgct attgataaat taaaaccaga tttcactatt tcaacaaaat atttcaactg
    15181 ttttatatcc attttaaacc cccttcccca aaagtataat ttagattata ttttttcttc
    15241 taatgatatt ttataggaaa atcaacataa tttcattaat attttaacaa aatatttttc
    15301 taaatctact ataaattttt atcctataaa tttttcttat acactatata aaaaattaga
    15361 cttagacaaa acgctttttt cgccgtaatc tataattaga aacaggaaaa cctaaagcat
    15421 taggaataaa agtaattgaa atacttaaaa acaagcttgt attaaaaaag tactttatta
    15481 tagtgttccc taatttcttt attcagaaaa tttttaatta tcattcaaga aaggtaacta
    15541 ttagatatga aagatgtagt aatcgtatca gcagtcagaa cacctatagg aagttttgga
    15601 ggtgtattta aaaatacttc agctgttcaa cttggaacta tcgctgttaa agaagcgata
    15661 tcacgtgtaa gacttaattt atctgaaata gatgaagtaa taattggaaa tgttcttcaa
    15721 acagggcttg gacagaatgt tgctagacaa attgctataa atgcaggtat accaaattct
    15781 gttccaagct acacagtaaa taaattatgt ggttcagggc ttaaaagtgt ccagctagct
    15841 gcccaatcaa tcacatctgg tgaaaatgat gtggtaattg caggtggtac tgaaaatatg
    15901 agccaagctc cttatatcgt tccaactgct cgctttggta gtaaaatggg tgacataacc
    15961 atggtagata gtatgctaac agatggcctt attgatgctt tcaatcagta tcatatggga
    16021 ataactgcag aaaatattgc aactaaattt gaatttacta gagaaatgca agataaattg
    16081 gcattagaaa gtcaaaataa ggctgagaat gctataaaaa acaaccgctt taaagaagaa
    16141 atcgttccag tagatgtttt aatacgtcga ggaaaaatag aaactattga taaagatgaa
    16201 tatcctaaat taggtatgac atttgaaggt ttatctaaac ttaaacctgc attcaaaaaa
    16261 gatggtactg taacagctgg aaatgcctca ggtattaatg atggtgcggc tatgttaata
    16321 ctaatgagcc aacaaaaagc agacgaacta ggtattagac cacttgctaa aataaaatcc
    16381 tatgcttcag ctggtgttga acctgaagtc atgggtactg gtccaatccc tgcaactaga
    16441 aaagcactga aaaaagctgg acttagcatc aatgatatag acttaataga agcaaatgaa
    16501 gcgtttgctg cccaagcatt agctgttaaa aatgaacttc aaatagattc atcaaagtta
    16561 aatgttaatg gtggtgcaat agcattagga catccaatag gagcaagtgg agctagaata
    16621 cttgttacct taatttatga aatgcaaaaa cgtaaagttg aaactggact tgcaacactt
    16681 tgtattggtg gtggtcaagg aatatcaatg gtggtttcac gataggagga aatatgaata
    16741 aaatagttag cattgatgaa gctctatctc atgtaaaaga tggagataca ataatggttg
    16801 gtggattcat ggccaatggt tcaccagaaa atttaattga ttatttatgt agtaaaaata
    16861 taaaagattt gacactaatt tgcaatgata ctggctttat tgacaaagga gttggcaaaa
    16921 tggttgtaaa taagcaattt aaaaagataa ttgcttctca tgtaggatta aataaagaaa
    16981 ctggaagaca aatgaataac aatgaaacag atgttgagtt agttcctcag ggaactcttg
    17041 ctgaacaaat acgtgcagct ggttatggtc taggaggtat actcacaccg actggtattg
    17101 gtactcttgt tgaggaggga aagcaaaaaa ttgttgtcga tagcaaagaa taccttcttg
    17161 aaaaaccaat ttatgcagat gttgctcttc tatttgcatc aaaggtcgat aaagcaggta
    17221 atctagttta taaaggctct atgaacaatt ttaataattt aatggcatct gctgccaaaa
    17281 ttacaatagt tgaagcagat gaaatcgttg aaattggtga tattgaccca aatgaagtta
    17341 atacaccagg tatttttgtg aattacatcg ttgaaggagg taatttataa tggataaatt
    17401 agaaatgcaa gaatatattg caaatagagt atctaaagaa ttaaaagacg gagccgttgt
    17461 aaaccttgga ataggtctac caaccaaagt agcaaattat attccggata atgtaaacgt
    17521 tatcttacaa tctgaaaatg gctttttagg tttaggttca gccgaagatg gaaattcaag
    17581 tgatgaaact attgtaaatg caggtggtca accagttact atacttcctg gaggatgttt
    17641 ctttgatagt gctacttctt ttggaataat cagaggaggt catgttgata taactgttct
    17701 tggcgcactt caggttgaca aatatggcaa tatagcaaac tatatgattc caggtaagat
    17761 ggttccaggt atgggtggag ctatggacct agtaactgga gctaaaaaag ttattgtagc
    17821 aatggaacat acttcaaaag gttctgcaaa aatattaaat aattgtactc ttccacttac
    17881 tgcaacaaat gctgtagatt tgatagttac tgaaatggga gttatggaag taacatcaga
    17941 tggaatttta cttaaagaaa taaatccagc tttcacatta gatgatgtta tttctgctac
    18001 tgaagctcct ttaatattgt cagattcatt aaatggaaaa atatctgttg tttaaaataa
    18061 ttaaaactag aaaataaaaa tctcttagac gtattacata taaatttgta ttgcttaaaa
    18121 tattaatata aaaataaaat aaaattggag gaaatgaaaa tggttaaaga taaagttgta
    18181 ttcgtaacag gagctgcaag tggaataggt aaacaaatag ctgaatcgtt cttaaaaaat
    18241 ggtgctaaag taatgttctc tgatataaat caagaagctt taaataatac cataacagag
    18301 ttacaaaaaa aaggttatga ttgcatgagt gtaaagtgtg atgtaactaa ggaagaagaa
    18361 attaaccacg ctatagacaa aactgttgaa aagtatggca gattagatat attaataaac
    18421 aatgctggac ttcaacatgt ttctatgata gaagatttcc caactgataa atttgaattt
    18481 atgataaaaa taatgctgac agcacctttt atagctacta aaaaagcatt tcctataatg
    18541 aaaaaacaag gctttggtcg tataataaat atggcctcaa tcaatggtgt aataggattt
    18601 gctggaaagg ctgcttataa ctcagcaaaa catggactta ttggactaac aaaagttgct
    18661 gctcttgaag ctgccgattc tggtataaca gttaatgctt tatgccctgg atatgtagat
    18721 actccacttg tcagaggtca gttcaatgac ctttcaaaaa ctcgtaatat accattggaa
    18781 aatgtcttag aggaagtttt atacccactt gtaccacaaa aacgcctatt agatgttcaa
    18841 gaaattgccg actatgctat gttcttagca agtgataatg ctaagggtat tacaggacaa
    18901 gcttgcttac tagatggagg atatactgca caatagttat tatatttagg gcttgaagaa
    18961 agtattcttc aggtccttct actaaggagg gatatttatg agtattatcg cgccaattgg
    19021 tataattatt ggtatcatag ctattatata tttttcagtt aaagaaattc atataactat
    19081 agctgctcca atagcaactt taatagttgt tattttaaat aaaatggatg ttgtaacgtc
    19141 aatgctaggt gctgacaaaa acaattatat gggtgcactt ggaaactata taatgagtta
    19201 ttttgcaata ttcttattag gctcaatact tgctaaattt atggaagaaa gtggagctac
    19261 tgtatcaata gctgatttta ttttaaataa atttggctct aatcatcctt atagagtttt
    19321 agttgctata tttatagtaa gttttatatt aacgtatggt ggtataagct tatttgtagt
    19381 tatgtttgcg gtcattccac ttgcaagaac tctatttaaa aaacttgata tttcttggaa
    19441 ccttattcag ataccacttt ggcttggtat tgcaacaatt actatgacta taattcctgg
    19501 aacaccagcc attcaaaatg ttatacctat tcaatatcta aatacgtctc ttactgcagc
    19561 tactatacca agtattgtag gtagtatagg ttgtgctaca tttggacttg tctatatgaa
    19621 atatgctcta aacaaaagta ttagaaaagg tgaaacttat gcaacttaca cttcagaatt
    19681 agaagaagaa tctttagata gagaattacc aggatttttt gcaagtattt ttcctctatt
    19741 agcactcgta attatagcta taactggaag tacttttggt tcagaattta tgaaaaaaaa
    19801 tgtaatttac atagctttaa tagttgcaat tcttttatct gcttttttat tccataaatt
    19861 tctatcatct aagatacaga cattgagcat aggtgctagt ggttctatag gtcctatatt
    19921 tgctacatct tctgcagttg catttggagc agtaataatg gctgcacctg gttttgatgt
    19981 atttagcaaa cttataatgt caataccagg tagtccactt ataagtctta ctgttttgac
    20041 ttcaacaatg tcagcaataa cagggtcctc ttctggtgca ttaggaatag taatgccaaa
    20101 ctttgcagat tattatttaa gtactggtct aaatccagaa ttaattcacc gtgttgctac
    20161 aatagcttca aatatactaa ctatagttcc acaaagtggt gtttttctta cattcttaag
    20221 cttatctaag ttaacacata aaactggatt taaagaaact ttcattgtag ttgcagttgg
    20281 ttcactaata gcagaaatta ttgtaattac attaggatta ctaatgtatt aatataaaac
    20341 aagttaaaat tcataatatg aaaggtggtg tctcaaagtg aactttttac ttaatgagac
    20401 actcttttta tatgaaataa catatgccta tcatttaaac aataaaaaag gccttatctc
    20461 tattttgaga cagacccttt ttatttctta aagatttaaa taattataat ttgttgctaa
    20521 tacgaaagca tctactgaat aaatttaacc aaataaaaaa aaatttatca taactataaa
    20581 tttacgttat ggaattatta taaaaatatt attttctcta actaagtaat tccatactat
    20641 acaggctttt aatacaaatt gtcaaacaca ctgaaatata cctatttatt ccatatatat
    20701 cttttttaca tatctttaca gtaaaaaaag tcttaatctt atgttagtat agattcacgg
    20761 gtaaccccct acactaaagg ttacaaatca gtaacatttt tagaattttt agattaagag
    20821 gtgtttaatg atgagaagaa atataccaac aaaattaaaa actatattag ctttaggagt
    20881 gttaacttta gcaacagctt caagtgctgt tactgcaagt gcttcatcac ttgaaaataa
    20941 tgtaaatata agtaacaaaa gtttagtata tagtaatact aaatacaagt tttctgcaaa
    21001 accaaattgt tctgtaaaac cagaacttaa gcctgataaa gataactctt gtaaggataa
    21061 gaatcataat gacaagaatt gcaacaacac taataaacct gaggacaaca ataattcaga
    21121 ctctactaat aagcctgata acaataaacc tgagtctaat aaacctgaag acaacaataa
    21181 ttcaggctct actaataaac ctaataataa taaacctgag tctaataaac ctgaagacaa
    21241 caataattca ggctctactg ataaacctga taacaataaa cctgagtcta ataaacctga
    21301 agacaacaat aacaataatt cagactctac taacaaacct gatgataata acaactcagg
    21361 ctctacaagt gaaagttttt cagcttacca aaaagaagtt gttgacttag taaatataga
    21421 aagagctaaa gctggattaa atcctcttac tcttgattct tctatatcaa atgttgctac
    21481 aaaaaaatct caagatatga ttgataacaa ctatttttca cacaattctc caacttatgg
    21541 ttctccattt gacatgttga aaaaatttgg aataagctat aagacagcag gtgaaaatat
    21601 agctatgggt caaaaaactc ctaaagaagt tgtaaacgca tggatgaatt ctgaaggtca
    21661 cagaaaaaat ataatgaatc caaatttcag taaaataggt gttggagtag cacaaaaaag
    21721 tggtggttca atttattgga cacaaatctt tgtaggataa aatactcttt ttccatacat
    21781 atcaaaagag tatctctaag tgataaaaaa cacaaatgag atactctttt gtatctattt
    21841 taaaattatc aaatccaaat atacatacta taaatattat ttgttctctt aattttagtg
    21901 tgtagaacaa tatataaaaa gtattggcaa gatgaaaatg gaaaacttaa aaaagatatt
    21961 taataggtaa aaacaaaatc ttaatatctt cataaaaaaa ggctgtctca aaatagagat
    22021 aaggcctttt tcattgttga aatgattaaa atatatttga tttcatacaa aaagagtgcc
    22081 tcatgaacta aaaagctcac tttgagacac tctcttttat caatttatat aagcattata
    22141 atactttaat attattcagc taattttttg tatccttcat atctttcttt agcatcagct
    22201 tcagctttag caaataatcc atcagcttct tctgggaatt gtttagctat agcagaatat
    22261 cttacttgtg caagtatgaa atctttgaag cttgcagttg gttcttttga atctaaagtg
    22321 aatgggtttt taccttcagc ttttacttct ggattgaatc tgtataaatg ccagtatccg
    22381 caagcaacag cttgtgcttc gttagctaca gttcttccca taccttcttt taatccatgg
    22441 tttatacatg gagcataagc tattattaga gatggcccat catgtttttc agcttctata
    22501 acggctttga taaattggtt tttatcagca cccatagcaa cttgagctac atatacgttt
    22561 ccatatgtca tagccatcat tcctaaatct ttctttctag attttttacc tgaagcagca
    22621 aacttagcca tagcagcagc tggagtagct ttagaagctt gacctcctgt atttgaatat
    22681 acttctgtat caaatacaag tacgtttaca tcttcaccag atgcaagaac atggtctaat
    22741 ccaccgtaac cgatatcata tgcccaaccg tctccaccta gtatccattg agatctctta
    22801 actagataat cttttctatc ttttatagct tttactaact ctttagcttt ttcatctgta
    22861 caatcacaag ctggtttttc taataattca acaaccttag cacttgctac tttagaagct
    22921 tctccatcat ttctagaatc taaccactct gtaaatactg ctttagcatc ttcacagata
    22981 cccatagaaa ctaactctgt catagcatct actattttat ttcttatttg cttaacagct
    23041 gtgtacatac caaatccata ttcagcatta tcctcgaata aagagtttgc ccaagatgga
    23101 cctttacctt catggttaca agtataagga attgatggtg cagaagctcc ccatatagaa
    23161 gaacatccag tagcattagc tatcatcatt ctatctccat ataattgtgt aactacttta
    23221 atgtatgcag tttctccaca tccagcacat gctcctgaaa actccattaa tggttgtcta
    23281 aattgacttc ctttaacagt gtttacaggc ataacatcac cttttggagc tacctcatca
    23341 ggatttactg caaatgccca attatctaac tctgtatcaa gttgagtatc tattggcttc
    23401 ataactaaag ctttttcttt agctggacat atgtcagcac agtttccaca tcctgtacag
    23461 tcaagtggac taagttgcat tctatatttt aatccttcta atcctttacc taatgctttt
    23521 ttagcttcaa atccttctgg agcattagct aattcttctt cagtaactaa tactggtctt
    23581 atacatgaat gaggacatat aaaagaacat tggttacatt gaatacagtt atctattatc
    23641 cattcaggaa catcaacagc tataccacgt ttttcataag ctgcagtacc acaagggaat
    23701 gttccatctt ctataccatt gaatgtactt actggtaagt tgtttccttc ttgtgcagtc
    23761 attggtctta gtatattttt tataaattct ggttctttaa cttctccttt atctgcatca
    23821 acagcatttt cccaagaagc tggaacttca actttaacta aagcatttat tcctgcatca
    23881 acagcagcat agttcatgtt aactattttt tcaccttttt taccataagc tttaactata
    23941 gaatctttta agtatttaac agcatcttct tctggtatta tttcagctaa tttaaagaat
    24001 gcagattgca ttatcatatt tattctgttt ccaagaccta tttcttgtcc tagtttaaca
    24061 gcgttaactg tatagaattt tatatcattt ttagctatat attgcttcat atgagctggt
    24121 aaatgttctt caagtcctgc ttggtcccat atagtgttaa gtacaaatgt accacctttt
    24181 ttaagacctt ttaataaatc atattgataa acatatgatt gtttatgaca ggcaatataa
    24241 tcagcttcat ctattaaata agtagctctt attggagtat caccaaatct taagtgagac
    24301 atagttatac caccagattt tttagagtca taatcaaagt aagcttgagc atatttttca
    24361 gtatggtcac caattatttt gatagcttgt ttattagctc caacagtacc gtcagaacct
    24421 aatccccaga acttacatct tatagttcct ttagaagcta tttttattgg ctcagatgga
    24481 gttaaagaag tattagttac atcatcaact atacctactg taaatccatt tttaggttgt
    24541 tctgaaacta agttatcaaa aacagttttt atatctgaag gagtagtatc ttttgaacct
    24601 aatccgtatc ttccacctat tatcatagga gcattttctt ttccatagaa tacatcacgt
    24661 acatctaagt ataatggttc accagttgaa cctggctctt tagttctatc aagtacacat
    24721 attctttcaa cagtagatgg catagcatct aagaaatgtt tcattgagaa tggtctatat
    24781 aaatgaactt taactaaacc atatttttct cccttagcat ttaagtaatc tatagtttct
    24841 tctattgttt ctgttactga acccatagct attaatacgt atttagcatc ttctgctcca
    24901 tagtaatcaa ataaactatg ttttctacct gttaaattac tcattttttc catgtatttt
    24961 tcaactatac ctactatatt ttgatagtat ttattagaag cttctctagt ttggaaatat
    25021 atatctgggt tttgagcagt accacgagca actggatgat ttggagataa agcattatct
    25081 ctaaatgctt gaactgcttc aaagtttaat aaacttgcat aatcttcatt ttctagtaat
    25141 tcaacttttt ggatttcatg tgaagttctg aatccatcaa agaaatgtat aaatggtaat
    25201 ctaccttcta ttgcagctaa atgtgcaaca ggtgctatat cagctacttc ttgaactgaa
    25261 ccagaagcta ataatacaca tccagtttgt ctagcagcca taacgtcttg gtgatcccca
    25321 aatattgata acgcttgaga tgctaaagca cgagcactta catggaatac tcctggtaat
    25381 aactctcctg caactttata catgttaggt atcattaata ataaaccttg agatgctgta
    25441 aaagtagaag ttaaagctcc tccttgtaaa gaaccgtgga atgttcctga tgcacctgct
    25501 tctgactgca tttcaacaac attaactgtt tgtccaaata tattttttct tccttgtgat
    25561 gcccactcat caactacttc agccatagtt gaagatggtg tgattggata tatagctgct
    25621 acatctgtaa acgcataggc aacatgagct gcagctgtat ttccatcaag tgttttcata
    25681 aacttagcca taatatgtat cctccttgat agctacaatt gtacttgttt ataacaagtc
    25741 tattttttta tatttacata taatttacac aacaaaacta taaacacaca tatagtcgcg
    25801 caaatactct atcttctaat ataatactac agattagata aaattccttt gaaaattgta
    25861 aattaattac atatttaagt agattttttt taaaattaaa tatgttaagt ttttttcata
    25921 attttgtata caatatataa taaattaagt tagatttttc aaactttttt atctatttgt
    25981 tcatattagt ataaaaagta aaatgcttat ccttattaca tgtaaatttt tatatatata
    26041 attttaccat attacatcat atttttgtaa gtttttatta cattttttat aatacccctt
    26101 tattttataa attaatctaa tcgattaaat ttatcctata gaaatttatt acatttaaaa
    26161 atatgctagc aaataaaata tctactagca taaagtctat ttataatatt aatcaactat
    26221 taattctact aaatctccat ttttaacgcc tgctgcattt ccctcttcca tatcaacatg
    26281 catttctaga ttaaagctgt cactagctct tactaataca ttttcaaata ctaatgctct
    26341 tggtccagat gttttaactt ttactatttg cttatcagtt acaccaaatt tagcagcatc
    26401 ttcattgctc atatgtatat gtctagaagc aactataact ccttctttca tttcaacttc
    26461 tccagcaggt cctactaatt taacacctgg agttccagct atatcaccag attcttttat
    26521 tggtgctttt actcctaatg ggaaaccatc tgctaaagat atttcaactt gagtatgagc
    26581 tctagctggt cctagtactc taactccttt taaagttcct tttggtccta ctaaatccac
    26641 tttttcttca caggcatatt gccctggttg agataatggt ttaaatttag ttaactcatg
    26701 acctgcccca aataatacgt ctatatcagc ttgactcaaa tgaacatgtt tattagataa
    26761 tgctattggt aatttcattg aaattccctc cttttaatta tttaaattat tgcaatacaa
    26821 ttatacataa tattacctac aaaatcaaat tatattgaat caatatatat ctttaaattt
    26881 ttatatatta tatctttaaa ttcttgatta tctatataag atacatcatc tgtattgttt
    26941 aaatatatat tgtgtaaaag tttagtattt atactcttaa agaaaaaatc catcacaatc
    27001 tgtccacctt taaactgtga atcatatgat ttactccccc ccactgcaat atacattcct
    27061 atccgtttct tacttctatc aattgaaggt ttatttaata catatttact tgcatatata
    27121 gcttgagttc tatcaacaac agtctttaat ttagctgata ttgaatcaaa atgtacagga
    27181 cttattacta tagttccttt tgaatcatca aacattttat ataatggctt catatcatca
    27241 ttaaacttgc aataccctgt tttttcacaa aatccacatg ctgtgcaata ttcaatatcc
    27301 atattgtaga tattaaatat tttacaactt aatccacact cttctagcat agcttttact
    27361 tcatttgcta taaaactgca atttttatta attcttggac ttccatttat tattataatc
    27421 tctggacttt taagtccttt tttatcgttt gaatccataa tcaatctacc tatcctttaa
    27481 cataactatt aaaatatcat catcataatt atcttttgat gagaagttct ttaaatccaa
    27541 ttttagattt tctactatta actgttgatt taatctaaaa ttttctttta gaaagttttc
    27601 taatcggttt ataccatact cttcttttga tgaatttttt atctctaaaa ttccatcagt
    27661 gtacatacat accattgcat aatcttccat tgaaagagta ttattttcat aagtagcatc
    27721 ttccattatt ccaataggta tgcccttttt acaatttaaa ttattttcta ttgtgccatc
    27781 tttctttaca actacaggac tatagtgacc tgcattagac acagtaatca tattttcttt
    27841 agtatctata atacctatca aacaagttgt aaatactccc atcttgtcaa attcatcata
    27901 taacatccta tttaaatttg tcattatttc tcttggtgta ttgtattgat tgcataatac
    27961 tttaaaagca ccttttatca ttgcaacaac ataatttgat accattccat gtcccattac
    28021 atcagcaaca atataaacta tatgttcgtc atccactttt gtagcataat aaaaatctcc
    28081 acctactacc cttgctggtt tatggtaaaa ataaagttct ttctcatcat tacaatccat
    28141 tttaccttta tccataataa gctgttgttg tttatttaat atgttcaact cgttttcaag
    28201 aactttgtgt tttacttcct tttcattatg ttcatataat tgcataccaa cagaaacttg
    28261 tttggacaat atactaagaa tatttaaatc atcactagta tagtttttgc tatttgcaca
    28321 aactatacat cctatagtat catttttatc ctgaagtttg taatatatat aactttccaa
    28381 atttattccc ttttttgagc atggttttaa attatttaga taccccccca atgactcttt
    28441 ctttaaaaac atatcaatca taggatataa atttgttttc tttttttgct caacattata
    28501 acaactaact atagcatcct tattatgatg actatttata attattaaag cttctttact
    28561 attagtaata ttacacgctt gtttacttaa attttccaaa aagccatcaa tactcttatt
    28621 aaggtatagt tttttagtag cttcatttat ggcacttatt gagcctctta gtttatcaat
    28681 taaaatttgt acattattat ttttattagt tatttctatt gataaactta agagtgatgc
    28741 aacagatgat ataaaatcta tatcttgttc tgttatataa tcatcctctt ttaaaaaaca
    28801 agtcataaat ccaacaacta cgttatttat tataaaagga aaaactattc tcccaatata
    28861 accttcagct tttgctaaat ctcgttcacc ttctgctcta gaatcttcaa atacattttc
    28921 cacatatatt atcttttttt catttacagc ctcatgtata tactctggat atgtgtcaaa
    28981 atcaattttt gcacctttaa gactatttat tggaaaaatt tgaggtacat attctaaggt
    29041 ttgcgaacat actaagtagg catatttaga atcattttta taaaatagat tcacacatgc
    29101 ctttgtaggg tgaacaactt ctaacatttt ttctattatg tcatctttta tattaaaaaa
    29161 atctgtagat tcagtcacca tagcggatat ttccactaaa ttcttcattt tataactgcc
    29221 attagtcata ctcaatttct ccccctatat attaaactat ttattagtat tttaacatac
    29281 attctttata gtaaaaacaa aaacaacgac aaaactagca actaatatcc agttaaatcc
    29341 tgtcaataat cctataagaa gtaatgtaat taatgcattt agacctgttc tccctttttc
    29401 atccataaaa gttattttag agatgtcgat attatttttc acattattta tatgctcttt
    29461 cacttgttct tccatatttc ttttatatag tatataaact acaccaacta aaaacactcc
    29521 tatacctaaa accatgttta aattaaaagc taccataagc aatattgaac caaaagctaa
    29581 ctttaaaagc ttaaaaaata cactatctat ctttagagtt atatttctga tttctttaca
    29641 ttcatctctt aaagaatatc cacttgaatc taagttatta atctttgttt ttacaatttc
    29701 agaataaaaa agctttatta tcccatttaa cattttcatc actcctaaat attttacata
    29761 tatttgtaag ttttacaatt ataatatttt ttatacttgg taataataaa aaataacaaa
    29821 tattttatta tttagtattt attgtaaata agttattgaa gcttgattag ttaagtatag
    29881 atttttaata aatattttta taatttagaa ggaagtggtt ttgtgcaaat agaacaactc
    29941 gatttagaaa cgagaaataa aatatactgc tatactaaaa aaattcttag aaaatatcaa
    30001 aaaggcataa catctggaaa attaacagca gataaatttg cagataatat attgtctcaa
    30061 gattttatca gtagtatact taatgaaaaa atagtcaatg agacaaactt taagatatct
    30121 tatagaaact atatagaaac tttaataaat attcaaaatg aaaatttatc taacttaaga
    30181 aaaaaatcta ctaaaactgc aaaatgtttc aatatctctc aaataaccca attaaaaaac
    30241 ttactaagta atactggtta taatttatta attccatata aatacttaac tgctagagat
    30301 atagaaggaa tagttactct tattaatact gggtctattg aacttggaaa tgagagaatc
    30361 tacaattata tatcaaaagt ataataattt aaacctatta taaaaaggca atttatttaa
    30421 taaaaaatat ttttaatatt aaataaattg cctttttaat tttggattaa atactgctat
    30481 ccaagtgctt tttctagttt agataaatgt tgtattggat aatattttag tagctcttta
    30541 aattgttcta ctgataaacc ttctgttgtt gtatatattc ttattttttc atctaagtca
    30601 ctattaataa atttgttttt taaatcctca aaatttacac tactcatttt acatcactcc
    30661 ttatgtaaca tattcatttg tattatttta tccaaataca taaaatttat tacataaaaa
    30721 tatatctatt taataattta ataaaaaaaa gctatctcaa tttagaaatt actttctata
    30781 tttgagatag ctctagctaa actaatagcc aatagttata aataattatt tataactatc
    30841 tgttgctttt tccagccatt tgttgttcag ccatttcaac taatttttta gtcatgtatc
    30901 cacctacata accattttct cttgcagtaa ggtttccttt atcaacttgt tgataattac
    30961 tcataccaag ttcattagct atttctaatt tcatttggtt taaagcctct tttgctcctg
    31021 gtactactgt tctgttgttg ttattgttac ttgccatgtt gattaccttc cttctgttta
    31081 atttattttg tgttgctgat aatattatgt gattttaaac atttttaatt caggtaattt
    31141 ttagcaatta aaacctgcca aatatccagt agaaaaagct atttgcaaat tgaatccacc
    31201 tgtatatgca tctatatcta ttacttctcc agcaaaaaat aaaccttcta ctaattttga
    31261 ttccattgta cttgaattta tttcgtttac ttttacacca ccagaagtta ttatagcttc
    31321 ttcaataggt ctgtatctct ttactgtaaa ctttaaattt tttaataaat ggactaagtt
    31381 ttttctctgc tctctagaaa tttggtgaac aaccgtattt ggattaattt cacttaagtt
    31441 tattattata ggtattagtt tttttggaag taaatcatct aatgcttttt caaatttttt
    31501 atttgtatac ttctgaaaat ctttttgaac tcttttatcc aatttttcct catctaatgc
    31561 aggttttaaa tctaataata tagtgtaatt ttctttcctt gtatctttca ttctacaact
    31621 tgcagacttg attataggtc catctaaacc aaatttagta aattcaagtt ctccaaaatc
    31681 actataaact ttcttttgtt tgctattaaa cacccttatt tcaacatttc tcaatgataa
    31741 tttttctaaa tcttttgtaa aactttcttg tacttctata ccaattaaag atggttttgt
    31801 atctataatt gtgtgcccct gtgaaattgc aaatttatat ccatcaccag tagaaccagt
    31861 aagtggataa cttaatccac cagttgctac tacaactgaa tcacacttaa tttctttttt
    31921 atcgtttaaa attacttttt caattttatt atttttagat attatttttt ctactttcga
    31981 atttaataat atatttactt tcttactttt aagttgtctt tcaagagcat ttacaatatc
    32041 aaatgcttta tcactctctg gaaaaaccct ttttcctctt tctgttttag tctttactcc
    32101 aagattatta aacatactta ttacatcatc atttgtaaaa gtataaaaag cactataaag
    32161 aaattttcca tttgtaggta cattttctat taattcctct atttcacaag cgttagtaat
    32221 attacatcta ccttttcctg ttattgcaag tttttttcct aatttatgat ttttctcaat
    32281 taatgttaca tcatagcctt tctcacatgc agttgatgcg gctatcatcc ctgctggtcc
    32341 accacctatt attactacct tcttcaaata atcactcttt tctaccctta atttttttaa
    32401 tttaaactat tgactctata ccttaaaatc aatttaattt taaaagttta aataatgctg
    32461 gagctcctgc tattccataa aaaacaacta ctgtattctt aataaaattc ataaaaatta
    32521 tactcatagt agagtcactt atatttaaag tgactattaa cattgaaata aacaacttaa
    32581 tgccaatata taacattcct attgttacta tcccaaccaa aaacctacat attcttgaaa
    32641 aaattatatt tttactcccc ttatttttat tgctaaaatt tatttttttg ttattattat
    32701 ctgtattaaa tttaacaaat ctatcctcaa ccatatatcc aaatacaaat cctactatta
    32761 taccaaattt ttcaaaatat tcagggctat ttagaaatat agccactatt ataaatggta
    32821 taagtaaaac tacaagtaaa atataatttt tattatcatc aatatagtca aatattttta
    32881 caaatacaac tgtaaaaaat attcctaata tccaacccgc tatcacatct attggccaat
    32941 gaacagctaa atacagccta gacaatccta ctcctaaaac cataactgca cctattaaat
    33001 gaagatatct ctttttaaat atagtcataa tactaaccca aaatgttgtt gcagtttgag
    33061 tatgagcact tggaaaagaa taaccaccag cagtagaaac tctcaatgat tcaagacctt
    33121 ttattcctat tggtctggga gcttttacaa actctttgat gcctaaattt acaacaaaat
    33181 ttccaattat agcaaacagt attttctgtc cacatttttt atttatacac cagtatataa
    33241 ttgttgtaat taatattaat aacggtgctt ctgtgcttat tgtaaagaca agaaatataa
    33301 agttcaaaat tggatttctt aaattttgga aaaataataa tatatttaac tgaatatcca
    33361 tatagatgtc tcctttacta aaattttata taattaacta aaaagcacta actcccttag
    33421 aaattagtgc ttttgattca catttaaatc tattctacat cctcaactat acctatataa
    33481 ggtaaatttc tatacttttc agcatagtct attccataac caacaataaa tttgtcttct
    33541 ataacaaatc caacatagtc tacaggtata ttagcttttc ttctctgagg tttatcaagt
    33601 agagtacata gttttaaaga ttttggattt ctagttttta aagctgcaac tagattactt
    33661 aatgttaaac cagaatctat tatatcctct acaatcaaca cattctttcc ttcaatatct
    33721 acatctaaat cttttaatat tttaactgtt ccagaagact ctgttgagtt tccataactt
    33781 gtaacactca taaaatctat atttacatcc aaatctatac atcttattaa atctgaaaca
    33841 aatacactag ctcctttaag tatacctacc actagtaaat cttcgccttt aaagtcttct
    33901 tctatctttt ttcctaattc ataaactttt tcttttattt gttcttctgt caacattttt
    33961 cctgtaactt tgtacatttt gtatcctccc gctttccttc tattaactta tatattatag
    34021 cataccaaaa tatcaattaa aatctttttc atcattttta aacctaaatt aacagcttat
    34081 ccatactttt ttgcttttta cattaaaaat ttggaataac gattcctttg tattataatc
    34141 tatatgtaca tatattaatc agttattttt atcgattata taattttatt tttggaggta
    34201 cttttatgag cacaaaccat ggagctaatt tatatagttt atctagtaag tatggctttt
    34261 caaaggaaga atttatggat tttagctcaa atataaatcc ttttggtaca tcaagtttag
    34321 ccaaacaata cattgtaaac aatatagata tggtatctat gtatcctgac cctgattaca
    34381 tagacttaaa aacttctata tccaattact gcaaatgttc tatagataat atagtacttg
    34441 gcagtggagc tacagaacta atttcatcat ttatacatac aattaaccct aaacaggctc
    34501 ttttactctc tccagcctat tcagagtatg aaaaagagtt atctaaaatt aattgttcta
    34561 tagaaaaata ctttgcaaaa gaagaagata attttcatat aaatttagaa aatcttataa
    34621 aaactatcaa tgctaaagat tatgatttgg tagtaatctg taatcctagt aatccaactg
    34681 gatttgcatt tactaaagta gaagtgagag aaattttaaa aaatacagac tcatttttaa
    34741 tgattgatga gacttatgta gagtttactg atacagatac atactcttgt actcaattag
    34801 ttgatgatta ttctaattta ttcgttataa gaggtacatc aaaatttttt tcaacgcctg
    34861 gaataaggct tggatatggt cttatatcta acactaatgt caaaaatgaa ataaataaaa
    34921 atctagattt atggaatata aacataatag cttctaaaat gggtgaaatc atgtttagtg
    34981 atttagattt tatatcaaat actatttcct taatgaacac tgaaagagat tacctgctta
    35041 aagagcttaa gaatataaaa agcttagata tatacaatac taaaggtaat ttcatattgt
    35101 gtaaaatcaa gacaaaggaa ttgactgcta aatctcttcg agagcaactg cttccacaaa
    35161 aaataataat aagagattgt tgttcatttg aaggtctaga tgagtatttc tttagagtat
    35221 gtatattaaa acctaatgaa aataaattat taatatcatc tcttaaagca atttttaaat
    35281 aaaaaaatca ctttatttta taatttttta taccataaaa aatgagctat ctctatttga
    35341 gataactcat ttttgattat taaaaaacta agcactttgc tcttctttat ttcctttttt
    35401 actcattcca tcatttattt tatctactac tactgctatt gcattatctc ctgatacatt
    35461 acatgctgta ccaaaactgt cttgagttaa gtataaagct atcattaaac ttgcaagccc
    35521 accatctgat ggtattccaa ccattggtag gaaaggaagt gcactcatta tagccccacc
    35581 tggtgctcct ggtgctgcaa ccattgctat acctaacatt gcaatataag ggaataatac
    35641 acctattcca tgtggcatac cattcatcat taaaactgca gttgtaaatc ctgttattga
    35701 tatcatacta cctgataaat gtattgttgc acaaagaggt acaacaaatt ctcttatttg
    35761 cttacttact ccattttttt ctgcacaacc aacattaact ggtatagttg ctgctgaaga
    35821 ttgagttcct atagctgtta aatatccagg tacttgattt tttaacattc ttactggatt
    35881 ttttcctgaa aatacacctg ctgctgtgaa ttgacatgcc atatatacaa aatgtaaagg
    35941 tattactact aagtatactt tccagaatac ccctaatatt gtccatactt cccctgctat
    36001 agttatattt gcaaaagttc ctgctatgta taaaggtaat aaaggtataa tcactgttga
    36061 caacgttttc gttacgattc cttgaaattc ttggaagaaa ttaaataaag tctctccttt
    36121 tccatgattt cttaaagctg atattccaag tcccattaca aatgcaaata cgattgcagc
    36181 tgttacatct accataggct ctaatggaat tgtgaatatt ggagctaaca ttcctgcctc
    36241 tgggtctcca atttgacttg caacacttgc atcaataaaa tttgggaaga ttaatgtaga
    36301 tactgcaaat gataataatc ctgctattaa ggtagaacca tatgctattc ccgtagttat
    36361 ccccaataac tttcctgcac ctgttgctaa atcagctata cctgctgtta caaaacctat
    36421 aattataaat ggtataacaa attttaggaa tgctgagaat aaagtactaa ctgttactaa
    36481 tatttgtact attatttctg gtgcataact tccgattaaa ataccgacta tgataccgat
    36541 gattagtttg ggcactaaac ctattttttt cttcataata tttaccctcc ataacttttt
    36601 taaattaagc attcttgcac attgaatact tagttaaata ttggatatcc acttattatg
    36661 ataatagagt tttacctctc cgtcaacaat ttaaccattt tttgttaata aaaaaaagaa
    36721 taacatatat aaattgttat tcttcttcat cttttataga aaattctaca ttttcaatta
    36781 tatttgaatg tttatctttt tttgcgataa ctctatactt attaatttct tcattcacta
    36841 tacctatact ccaaattaaa actatagcat catctataaa accaaatgcc cctgcaatca
    36901 tctctggaat tatatcaacc ccagatacaa agtaggatat agtaaaaatt aatacagaaa
    36961 ttacctttat tttatcaact atattgattc ttttatctgt cataaaatca ggtagtttaa
    37021 gtaaatcaaa tacaaaaacc aaaccaaatt tagcattttt aaatctgacc aaaatttttt
    37081 ttaaaatatc taaaactata ttaatgatta tttttaaaaa ctccatattt atcacctctt
    37141 ataattatag gttattctac attataaccg atataattaa tttgatgaac atattagata
    37201 taaattttat caattagttt atatttaaat tataataatt caattcctgc actactacaa
    37261 ttttgaatca tatcctgtgg ccatactcca acttgtactt ctcctatatg agctttttgt
    37321 aagaaataca tacatattct tgactgacca attcctccac caattgtata cggaagttcg
    37381 ccatttaaaa gcatcttatg atagtcaaac tcttttctct cttcacatcc agcaagtttc
    37441 aattgtctat caagagattc ttcatcaact ctaattccca ttgaagataa ttctaatgct
    37501 gaatctaaaa caggattgta aaataatata tccccattca attcccaatc atcataatca
    37561 ggacttcttc catcatgttt ttctcctgat attaatgttt ttcctatttg agttataaat
    37621 actgctttct tctcttttac aattttatct tctctttcct ttggtgataa ttctggatac
    37681 atatcttcta actcttgtga tgttataaaa gttatttctt ctggtaatat ttcttttata
    37741 ccttcataca tactacatac aaaaatttca gtatctttaa atactccata caatcttttt
    37801 acaacatctt ttagagtctt ttcatttcta ctacttttat ctataaccaa ttcccaatcc
    37861 cattggtcta cataaagaga atgtatattg tctaattctt cattttttct tattgcattc
    37921 atatctgtat ataaaccact accaacttca aacccatatt ttttaagggc taatcttttc
    37981 cattttgcaa gtgattgaac aatttctgca tttttattca taaatggtat atcaaagctt
    38041 acagcctttt ctccatttaa attatcattt gcacctgttt ctggcaatac aaataacgga
    38101 gaagaaactc ttataagatt taattcatta cttagtctca tttcaaaaaa atcttttaat
    38161 ttttttatag ctatttgtgt atcaattacg ttcaaactac ttttataatc ctttggtatt
    38221 attgtgctca tatcattcac tccctatatt tatatatttt tccattaaaa aaacccttca
    38281 tccctatata aaagggacga aaggttgctt ttccgcggta ccaccctgat tagtttgaaa
    38341 taaattcaaa cccagctcac aatgtacaga tattactcga tacaccccaa tatgataacg
    38401 gtttggttcc gactaagcct actcttttaa aatttcggtt agcaactcca ggatgttctt
    38461 cactatagac ttcatattgg tctcacacca tcaccaactc gctttaatta cattctataa
    38521 ctactcttcc tttcaaagtc tttaagagtt taattcaata aaatttagct ctcttatgtt
    38581 ttctatttat tcataatagt atgataagtg attagttaaa atttgtcaag tttattattt
    38641 ttttattttt aaacacttta taaattttta cctatacttt ataaattttc aaatatagtc
    38701 tttaatctgt ccatacattc ttttaataaa gactgtggac aagctacatt tattctttca
    38761 aatcctattc cttcttttcc aaatatataa ccttcatcaa ataatacatt agctttttta
    38821 aacattaaat cttcaagttc tacttcattt aatccataag cagtaaaatc taaccatgct
    38881 agatatgttc cttctggata aacaagtttt acttttggta ggttttcttt taagtattca
    38941 tcaataaatt gtatattatt atctaagtaa tcatttaatt cattaagcca gtcttcactt
    39001 tcattatatg ctgctattgt agctacaata gcaaagggat ttggtgaagt tgcaactcca
    39061 acagctgtaa cctcttcttg atatttcttt tttagttcat cattattcac tattatatta
    39121 gacaactgca ttcctgccag attaaatgtt ttacttggag ctatacatgt aactatttta
    39181 tccttgtact ctgggcataa tgactgtaaa ggagtatgtt taaatccttt tctaattaaa
    39241 tcagaatgta tctcatcagc tattatccat aggtcatatt ttttacatat ttctacaacc
    39301 ttttttaatt catcttctct ccaaactctt ccaacagggt tgtgaggact acaaagtatt
    39361 agtactttat tgttgctaca cttagctttt tcttctaaat catcataatc catcctataa
    39421 gtaccattct catatatcag aggattgttt acaacgtccc tattattgct ctttatctta
    39481 gcttcaaatg ggtaatacac tggtttttgg actattacac cttcattaga attagttaat
    39541 attcttacta aaatagcaac agcaggtaca acaccaggac taaaaataat atcttttcta
    39601 cttatttccc agttaaatct ccttttatac caatcacaaa ctgcattaaa gtatttatca
    39661 ctatcatttg aactatatcc aaatatttta tgttcaactt ttttacgtaa tgcttctatt
    39721 attggttctg cacattcaaa atccatatca gcaatccaaa aaggcaaacc atctttttgt
    39781 gcttttgttg aacaattagt tctaaaatcc cattttacac aatctgttcc tactctatct
    39841 attactctgt caaaattata cttactcatt ttttccctcc taagaataaa atttttataa
    39901 ttgttaagtc tattactaga tgcactttgg tattatttta caattcttaa tattataata
    39961 atatcacttt tttgtccaat agatttaaaa tataattact tttccatcat attttttcaa
    40021 tattccaaaa tcagtttcat tatatcaaaa taagattgct catattaatt attttctaat
    40081 taacataagc aatctttttc tataacttct ataattataa ttttaaatgt agaattatta
    40141 gtttggatgt tatcccaatc tcttttcaac caatctacac catatatcta caccatattc
    40201 taaagctttc gtatcaaatg ttacatttgg attatgaaat cctggtgtag catcagctcc
    40261 taatccaata tatgtagttt tacattttag tttttgtgta taataatgga agtcttctcc
    40321 accagaattt ttaaatatac ctatagatgc atcagctccc aatacatctt ttatagtttc
    40381 ttcagccaag tttacaagtt cttcatcaaa ttctgctgct ggtactccat ctttggaaat
    40441 tagtacagct tctgcattta tagaacttac aacagttttt acagcatgta ttgctttttc
    40501 ttttaattct tctattatat cattactttc tgcccttata tctaaagcca taaatgcttc
    40561 atctggtata ttattataag tttctccctt aactgatata tttgttactt ttatagaatt
    40621 tggtatagct ggatttactc ttattgcatt tattgcattc actgctaaac agacagcatc
    40681 tactgcattt actcctaaat gtggtttaga accatgacaa gtgatacctt taatcgtaaa
    40741 ttccattata cagctagcac aatggtacat tgcaggtatc gcctttccca acctacaatc
    40801 ttgtccaggt cttaaatgca tccctaccat ttcttctaca tcatccaaca aaccagaatc
    40861 aattactctt aatgctccat ataatttttc ttcacctggt tgaaaaagta tttttaactt
    40921 acctcttttt actaaatttc tctcttttat taactttgca gctgttaaaa gcatagaagt
    40981 atgaccatca tgaccacaag catgtatcat tttcttttct ccatctatga taaattccaa
    41041 tgcatccata tctgctctta tagcaaatac tggtccttct tttttagaat ctatagttac
    41101 tataatacca gttcctgcaa ctcctttatt aatattttca aaccccattt tttcaagctc
    41161 ttttaaaaca aactctgatg tcttatattc ttcaaatcct ttttctggta tagtgtgtag
    41221 ataatcatac actttatgta catactcaat ataatctttg ttagttccca taatctagct
    41281 cctccttaaa atttaaactt tccatttata ttaaattttt attataatta cctatactac
    41341 gaaataattc attataagca ttgcaataca accatttaaa gttgctataa caaacatatg
    41401 tggataatat ttagttttta actcagctgt tcctaaaagt ctccccataa attgtatctg
    41461 cgctcctgaa agcaataaca ttggcaatag aattgcaaca tgtgcttgac ttatttgacc
    41521 tgctgtaaat aaacctgcga ttactccaga tgctcctccc attgatataa atattgtcat
    41581 tatagctgtt gctgcaacac ctggaagtcc aaagatactc attacaggtg taaatattgt
    41641 ccctattaca tccaaaagac ctgttaagtt gaacacagaa attatagcaa aaccgaagat
    41701 gacatttgga gccattgaat ttgtacttat ttggaatcca tttctagctc ctccaataaa
    41761 cgcatctatc acatttcctt ttttaacttt tacttctttt tttgctacct cactcatgtt
    41821 aagcttcccc cttgtcaaat ttatttaaat ataatctaag taagttcgcc ccaacaaact
    41881 tcataattaa tattaaagca agtggtataa atattggtac atctcctata aatggaaata
    41941 gtgctgctcc agaagcaaag aaattcgtaa ttgcagacgc acctgaaaat tgaaatgcac
    42001 aaaatacagc tttttgtttt tcatctatat acccatcatc tttaagttct ttagtcatag
    42061 atgcagctgc atctgtagac tgtaaactag ctatcaaagt tagtcctgca aatccaggta
    42121 accctaataa tggctttaat agtggactaa gaagcttttg agctgccttc attccatcta
    42181 agtggtctat aactctaact actccaagtg caaacataac tgatggtatt aatgtcaatg
    42241 catatagcca tccatctcga gggccagtac ctccatcacc tctaaagttt gcagctaatg
    42301 tacctacacc atcattaatt gttcccaatg ttccaaaaga acctaataca ttgttaaaat
    42361 ccaatacttt taatggtcct ggtgcatctt taaatattcc tgaaaaaaat aatgctccca
    42421 atattaatgc tacataccca taccatttta cctcaacttt ttctgttgtt gattctaatt
    42481 tactcatata aattctccct cctgtttaaa tgaattttat tagtatactt gtttaataag
    42541 caaaacttat gccaatacct taatgataaa gcctttgtat ttattttttt attgtaaatt
    42601 tattccagtg attaaatagc ttataattac ttacttttca agtttaaaaa tataatattt
    42661 ttttattaat tatttaaaat atacattaca atataaaaag ctattttcct tatcttgcaa
    42721 aaataatttc aaaataggtt tttataggat aaaatcctaa aaaaacctat tatttaatca
    42781 aatatataaa tttatactac tatttgttgt ataatataac ttgccttata ctttaaatac
    42841 tttaaaaata aatactattt tgttaggaga aataaaatgg ataaaaagaa gttaatcctt
    42901 ataacatatg ataaattgaa tagtgaccac tacaaagaag aattaacaaa cttttttgga
    42961 gacgaaataa tcatagaaac acaaaatata cttgatggaa taaaagaaaa tttagaggga
    43021 gatgtagtgc tttctctatc acctttaact agtaattttt taataaaaca ttttaaggaa
    43081 gatattgaaa ttattcatgg aacgaaagca ctttcaaaac ttggatatga aaaaatgatg
    43141 aaactgcctc caggtacaaa atcgttactt atgacaacca ataaaacttc agcatttgag
    43201 atggctactt atctatataa aattggtata aatcatatag attttgtacc gacatatcca
    43261 gattgtgatg aaatatatga tttagataca gctataacac caggtcagat tagatttata
    43321 ccaaaataca taaaaaatat tgttgattta ggttggagaa aaatctcatt agatacatat
    43381 atgtcattat tagtagtatt aaaactaaaa aatgagaaat ttatagaaaa attatacaaa
    43441 ttgtccaagg agacactaag tcatgatttc ttaaatacat cattagataa tatatctaaa
    43501 ttaaaaacta tactttacat gaccattgat gaaataggtg atggtctaat atttttcaat
    43561 acctttaata aggttacttt tgtaaataag tctttactta atatgttgga actagatgaa
    43621 aaacttataa aatcaccttc cttaatggaa tatatgccaa agagtttctt agataaaatt
    43681 acaaagaact taaatataga taatatgatt atatacatag acgaaataga taaaaaattc
    43741 atactttcta aaaaaccatt ttatctttat aaaaatatag agggttgctt aattacatta
    43801 aaagatgtaa acaatataga aatattagaa caaaaaataa gaagtgattc tgtcaaaaga
    43861 ggttatgtag ctaaatataa atttaataat attattggaa atagttctat tataaaagat
    43921 tgtattaaaa aggcaaaaaa aatggctctt acagataatc ctatattaat aacaggtgaa
    43981 acaggaacag gtaaagaagc ctttactcag tcaatacaca atcattcaaa tagaaaaaat
    44041 aaaccttttg tagcaataaa ttgtgcttca ttaccaagtg aattacttga aagtgaacta
    44101 tttggatatg aagatggttc ttttactgga gcaaaaaaag gtggaaaaaa aggattattc
    44161 gaattagctc atactggcac tatattttta gatgagatag gagatatgcc acatgattta
    44221 caggttaaac ttttaagagt cttacaagaa aaagaaatta gaaaaattgg tggtacaagt
    44281 attattccaa ttgatgtgag aatattagct gccacaaata aagatttaga gaaattaatt
    44341 atagaaaata aatttaggat ggatttattt tataggataa gtatgttcac tttagattta
    44401 cctcctctta gaaagagatt agaagatata cctcttcttt tagaatcatt tttaaaagaa
    44461 cttccctata aaaacataaa actcgataag agtcttttag aagctttaaa ttcatacact
    44521 tggatgggga atattagaga actgagaaat tgtgttgaat atatggctta catgggttcc
    44581 aattatctta caataaatga tttacctcaa aacatatctt caaaattaaa taataatcat
    44641 atgtcaagta atatgtccat attcaatgat ttaaaccaat atgacaaaaa catatgcatt
    44701 tctatcctaa aatcattgca catgaaacca atggggagga caaagttaat gaaatttatg
    44761 gaatataatg tcacagaata cgaagtaaga aatatgctag aatatttgac acgcaatgga
    44821 tacttaattt ctagtaaggg aagaaaaggc tcatcaataa ctgagaaagg cataaaaata
    44881 atagaaaata atataattta aacctatagt aattcatctt tactttatca aaatttatat
    44941 ataattataa ttttttattt ttctataaat tttttataaa ataataaaac ttatttatat
    45001 taaaaacgaa gctatctcaa catatggata agcttctttt taattgttaa aaagataaaa
    45061 atatatttga tttaacagaa aaagagtgcc tcataaacta aaaagttcat tttgagacac
    45121 gtccttaata ttaataaatt aattggctta aaattactga ttgataatta taaagtacct
    45181 ctgttaaagg agtaaattct gaaccgattc caaatcttaa ttctgtaaac gcctctaaat
    45241 aatctgggtc acatacatct tcttgtgtta aatcttgttt caccatttca gtagcaagat
    45301 aaattgctac tgatggtaga aaagcatcca ttggcatagg atatccacca agtctatttg
    45361 acatgccaaa agccttagcc ttttcactta tcatgtcgtt tattttatca taattttgag
    45421 catctttttc agatattttt agacccataa cactaggata tgtttgtatt ggtgatggat
    45481 tgctttgctc tgcaactata tactttaatt ctaaagcctt agtcaatata acttcatcca
    45541 tatactcatt tacaccaaat acatttatat cttttccata ttttttaact tgtttttcaa
    45601 tatcttcatt taaaaactgc ttaactttgt ttttgtcttc ttcagtattt atatttggag
    45661 tattaacttg tacaaatggc aatcctatat ttttgcaagt ctctttaatc atttctaatc
    45721 ttttggctat attaacatct ttcaaatcat ctgtagaggc ataatgaata aaagcttttg
    45781 ctcccatttc ttttgaacgt tcagctaata cttttcctct ttcctctgca ctaaccccta
    45841 gattcacatc aacaaattgg cttaattggt ttttatcatc tcccataggg gcagaaatag
    45901 ttattatttc tggacgcttt tcctttacct tttgtaaagc aggtaaaagc cctgcttggt
    45961 cagttgaaac tacaatagcc tgaacttctt tatcgtcagc caactttact atcttattaa
    46021 tagcactgtc tatattagaa gtaaaattct ctggcaatac aacatgcttt atagtttgtt
    46081 taactttagt ttgaccatct ttgtctttat caattttatt ctcttcctca tattcttttg
    46141 ccatctcttc caccatattg tattgtactt tattttcaga taatggttga gttaccactg
    46201 caactttaaa atcatctata attttctctt tatctttctt gcctacaagt aaactacaac
    46261 ctgtaagtcc aataattaaa catatggata gcataaatgc tgttattttt ctaagcatta
    46321 attaaaacct cctaaatttg tacattctta tcactattac taattttatc atacttcaaa
    46381 gaaaaattat attataaaaa attatataca tgctattata atccaatcta aaaagtacta
    46441 tgggtagatt aggtttagat taaacctacc catagtacta aaaattaata tattataaaa
    46501 ttaaattaat tacaaattat tatgctactt gtttgttgct ttttataaag ttaccaacta
    46561 ttccacccaa tatagctggt attacccagt taaagcccaa acctgcaagt ggcaagcttt
    46621 gtataaatgg aacagctcct gtcattccat ttatagctga taataaacta atcactaatg
    46681 taacatatgc tgcaccttta aaagcattat cattctttat atgtttacca gccaactcca
    46741 taacaactat tgctaataaa acaggatata tagtttgtag gattggaact gatatggcta
    46801 ttatagaatc aacacctaaa tttgatataa gcatactagc tacacatata cctataacca
    46861 gttgtttata actcgcctta cttattcctt caaaataagt tgatattgat gctgttaaac
    46921 ctgtagaagt agtcagacag gcaagtgcca caattacacc taaaattacc tttcctactt
    46981 gaccatataa caatatacta gttatttcaa ctaataaaga tgcttgaggt atactagtat
    47041 catataaagt tgaaactgtt gcacctaaat aagtaagacc accatatact actgctagaa
    47101 atacacaggc tacaaaacca gctttaatag tcattcctat ttggtcttta tctgatgtat
    47161 atcctttagc tattatagac atcaacatta tagttgccat agatgctgca cctagagcgt
    47221 ccattgtttg ataaccttgt gatatacctt caccaaatag atttttatct atcatatgct
    47281 caggactcat aggtcctgct ggagaaatta taccctttac tattaaaaca aataatacta
    47341 ataataacgc tggagttaaa tacttaccta ttatgtctat aaccttagaa gacctaatag
    47401 taaatgctaa agttagccca aagaagatta ttgaaaatac agctttagct actggaccat
    47461 ggtctccaaa tataggcata atacccattt catatgttgt tgcgcaagtt cttggtatta
    47521 caagaagtgc taaacatgct agagcagcta cactcattac tcttgctaac cctttaccag
    47581 ctctcctaag tacatcatcc atgcttccct tacatcttgc tgttgctata ataacaagca
    47641 ttgctatacc agcatctgca acaacaaatc caataagagg aattaaccag tctttgccgg
    47701 attccatccc taaaaatgga ggaaacataa gatttcctgc accaaagaac atagaaaata
    47761 aagcaaaacc aactataaaa atatctctca tctgcttact cacactaaat tacccccttt
    47821 gaaaatattt tgaatattaa gataatctta ctttattttc agaaaaatgt caatctggta
    47881 aaaattcaca cattgttaac gttttcccaa tttattttat gaatattttt ttcattttat
    47941 gataattccc atcaatttcg ttaaatcaat tgcttgtgac acatcaaaaa ttgctcctct
    48001 aatatcatct ataccaatct caattccctc aatattatta gtagtaaaat ctatgccaga
    48061 aagtttagtt ccattaaagt ttgctttttt taaatttgta tactcaaata ctagattgtc
    48121 accttttact tcttgaaata cagaattttc aaaatttgaa tctaagatag aaactatctt
    48181 aaatttagag aatgaaaagt tagaatactt acctaaaaca tccttgaaca aaacgttttt
    48241 aagtatacaa tcatcaaatc taactcctat caatttacta tttttaaatt caactctgta
    48301 tatacttcct ccatcaaaaa atgtatttga taaattgcag ttttcaaata ccacatcaag
    48361 taaatcaatc ttttttaaat tacaattttc aaaagacaca tttctaaata tacaagattc
    48421 aaagctaact c
//
LOCUS       DAYAOY010000021        47390 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYAOY010000021 DAYAOY010000000
VERSION     DAYAOY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.021"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(64..336)
                     /locus_tag="ACGI6B_001258"
     CDS             complement(64..336)
                     /locus_tag="ACGI6B_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750712.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            555..1373
                     /locus_tag="ACGI6B_001259"
     CDS             555..1373
                     /locus_tag="ACGI6B_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9750713.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1506..3626)
                     /gene="feoB"
                     /locus_tag="ACGI6B_001260"
     CDS             complement(1506..3626)
                     /gene="feoB"
                     /locus_tag="ACGI6B_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9750714.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3652..3879)
                     /locus_tag="ACGI6B_001261"
     CDS             complement(3652..3879)
                     /locus_tag="ACGI6B_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9750715.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4206..4646)
                     /locus_tag="ACGI6B_001262"
     CDS             complement(4206..4646)
                     /locus_tag="ACGI6B_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9750716.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4819..5787
                     /locus_tag="ACGI6B_001263"
     CDS             4819..5787
                     /locus_tag="ACGI6B_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HFG9750717.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    5939..6191
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6264..7472
                     /gene="tyrS"
                     /locus_tag="ACGI6B_001264"
     CDS             6264..7472
                     /gene="tyrS"
                     /locus_tag="ACGI6B_001264"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFG9750718.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(7533..8414)
                     /locus_tag="ACGI6B_001265"
     CDS             complement(7533..8414)
                     /locus_tag="ACGI6B_001265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9750719.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            8800..9744
                     /locus_tag="ACGI6B_001266"
     CDS             8800..9744
                     /locus_tag="ACGI6B_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9750720.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            9967..10512
                     /locus_tag="ACGI6B_001267"
     CDS             9967..10512
                     /locus_tag="ACGI6B_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFG9750721.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            10603..11751
                     /locus_tag="ACGI6B_001268"
     CDS             10603..11751
                     /locus_tag="ACGI6B_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9750722.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            11804..13069
                     /locus_tag="ACGI6B_001269"
     CDS             11804..13069
                     /locus_tag="ACGI6B_001269"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFG9750723.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(13355..14587)
                     /locus_tag="ACGI6B_001270"
     CDS             complement(13355..14587)
                     /locus_tag="ACGI6B_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750724.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(14590..15264)
                     /locus_tag="ACGI6B_001271"
     CDS             complement(14590..15264)
                     /locus_tag="ACGI6B_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750725.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(15272..16501)
                     /locus_tag="ACGI6B_001272"
     CDS             complement(15272..16501)
                     /locus_tag="ACGI6B_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFG9750726.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(16613..17617)
                     /locus_tag="ACGI6B_001273"
     CDS             complement(16613..17617)
                     /locus_tag="ACGI6B_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9750727.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            complement(17641..18315)
                     /locus_tag="ACGI6B_001274"
     CDS             complement(17641..18315)
                     /locus_tag="ACGI6B_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9750728.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            18603..19370
                     /locus_tag="ACGI6B_001275"
     CDS             18603..19370
                     /locus_tag="ACGI6B_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750729.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            19357..21294
                     /locus_tag="ACGI6B_001276"
     CDS             19357..21294
                     /locus_tag="ACGI6B_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750730.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            21519..23132
                     /gene="ggt"
                     /locus_tag="ACGI6B_001277"
     CDS             21519..23132
                     /gene="ggt"
                     /locus_tag="ACGI6B_001277"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFG9750731.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(23265..24116)
                     /locus_tag="ACGI6B_001278"
     CDS             complement(23265..24116)
                     /locus_tag="ACGI6B_001278"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFG9750732.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            24742..25635
                     /locus_tag="ACGI6B_001279"
     CDS             24742..25635
                     /locus_tag="ACGI6B_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HFG9750733.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            25635..27029
                     /gene="gltA"
                     /locus_tag="ACGI6B_001280"
     CDS             25635..27029
                     /gene="gltA"
                     /locus_tag="ACGI6B_001280"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HFG9750734.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            27395..29614
                     /locus_tag="ACGI6B_001281"
     CDS             27395..29614
                     /locus_tag="ACGI6B_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9750735.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            29761..30507
                     /locus_tag="ACGI6B_001282"
     CDS             29761..30507
                     /locus_tag="ACGI6B_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750736.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            30507..32387
                     /locus_tag="ACGI6B_001283"
     CDS             30507..32387
                     /locus_tag="ACGI6B_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750737.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            32506..33897
                     /locus_tag="ACGI6B_001284"
     CDS             32506..33897
                     /locus_tag="ACGI6B_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9750738.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            34004..35044
                     /locus_tag="ACGI6B_001285"
     CDS             34004..35044
                     /locus_tag="ACGI6B_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9750739.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            35533..36186
                     /locus_tag="ACGI6B_001286"
     CDS             35533..36186
                     /locus_tag="ACGI6B_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFG9750740.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            36262..36471
                     /locus_tag="ACGI6B_001287"
     CDS             36262..36471
                     /locus_tag="ACGI6B_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750741.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(36611..37177)
                     /locus_tag="ACGI6B_001288"
     CDS             complement(36611..37177)
                     /locus_tag="ACGI6B_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFG9750742.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            37338..37991
                     /locus_tag="ACGI6B_001289"
     CDS             37338..37991
                     /locus_tag="ACGI6B_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFG9750743.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(38149..38325)
                     /locus_tag="ACGI6B_001290"
     CDS             complement(38149..38325)
                     /locus_tag="ACGI6B_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750744.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
     gene            38853..39797
                     /locus_tag="ACGI6B_001291"
     CDS             38853..39797
                     /locus_tag="ACGI6B_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFG9750745.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            39819..40445
                     /gene="hisG"
                     /locus_tag="ACGI6B_001292"
     CDS             39819..40445
                     /gene="hisG"
                     /locus_tag="ACGI6B_001292"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFG9750746.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            40479..41528
                     /gene="hisC"
                     /locus_tag="ACGI6B_001293"
     CDS             40479..41528
                     /gene="hisC"
                     /locus_tag="ACGI6B_001293"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9750747.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            41531..42109
                     /gene="hisB"
                     /locus_tag="ACGI6B_001294"
     CDS             41531..42109
                     /gene="hisB"
                     /locus_tag="ACGI6B_001294"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFG9750748.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            42111..42716
                     /gene="hisH"
                     /locus_tag="ACGI6B_001295"
     CDS             42111..42716
                     /gene="hisH"
                     /locus_tag="ACGI6B_001295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFG9750749.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            42713..43435
                     /gene="hisA"
                     /locus_tag="ACGI6B_001296"
     CDS             42713..43435
                     /gene="hisA"
                     /locus_tag="ACGI6B_001296"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFG9750750.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            43417..44175
                     /gene="hisF"
                     /locus_tag="ACGI6B_001297"
     CDS             43417..44175
                     /gene="hisF"
                     /locus_tag="ACGI6B_001297"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFG9750751.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            44203..44871
                     /gene="hisIE"
                     /locus_tag="ACGI6B_001298"
     CDS             44203..44871
                     /gene="hisIE"
                     /locus_tag="ACGI6B_001298"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFG9750752.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
     gene            45673..47001
                     /locus_tag="ACGI6B_001299"
     CDS             45673..47001
                     /locus_tag="ACGI6B_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9750753.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT        18111 a         5260 c         7696 g        16323 t
ORIGIN      
        1 agaaatccta ttaccttttt attattaatt ttatcagtgt tttattataa atagcttaca
       61 taattacaca ttttttctga ttaaataata tgatgctatt gttataatag atactaatgc
      121 taatgatgtt attgcagtat ctaatcttct tattaataaa ctcatttcta tatattcaag
      181 ttctattaaa tactgataaa atatttctag tttaacagct ttttctctat ctatatttcc
      241 gtactttaac tctaatttat ctaaagattt tttattacaa taattaactt tatgtttaat
      301 taaatatctt attgtagcac ctattacaat tttcactcta acaagtataa atataatatt
      361 ccatccaaaa gttaagaggg gatatctttt ttatgatggg aaagggtaaa atatttataa
      421 ctgacatcta actgatattt aagctttaaa atcaaaacca tattgatttt ctaaaataat
      481 aactacttga tttagtaaat aataaagaat atagtaacta tatagacaac aaaaagaaaa
      541 ggagtgtata acccttggaa aaacagtatt ttacaacagg tgaatttgct aaattgtgtg
      601 gtatatctaa gcagacatta attttctatg acaaaatagg tattttttct cctgaatata
      661 aagataaaaa taattataga tattactcag tttatcaata tgatacactt gatatattac
      721 aatcattaag agaaatagga atgagtttag aggaaataaa agagtatata caaaatagaa
      781 ctccccagct ttgtgtgaaa atgcttaaag aagaagaaaa aaagataaga gaaaaaataa
      841 aaaaactaag aaagataagt tctaagatgc aaaatagaat aaatataaca gttgaaggaa
      901 tttctaaaat gaatagtgaa gaaatttata tatccaaaaa gtttgaagaa tatttagttc
      961 tatcaagcga tttgagtaat atgagtaata gtgattttat gatggaatta atagatttta
     1021 ccaattattg taaatcgaaa aatttgtatc aaggatatga gttgggagta atagtaagta
     1081 atgaaaatat aaaaaatggg gactatttaa gtatatcaag tttttatttg aagatagata
     1141 aaaaaataaa ggataaaaag ctatatgtaa agccagaagg aatgtatgct tgcataaaac
     1201 atattggaaa atatgaggat tcatataaat cttacgaaaa attgaaaaaa tatatatatg
     1261 aaaatgaata taaaattgtt ggaaattcgt atgaggagag tatacttgac tttttttgtg
     1321 aaagtaatga agataattat atgactgaaa tttcaatcca agtatccaga taagaattaa
     1381 aatagagata tattcaaatg ttaagtaatt ttacttatca ttgaatatat ctctatgctt
     1441 tataataaag tatataaatt tttaaatggt ttctctaata tatgtctact gtcagttaat
     1501 actaattata taaataaatt gtaaactgta gttgttaaaa aacaaacaac aaaagcaagt
     1561 atagtaggca ttaagaatcc taaggcagtc cattttaaac taccagtttc tttttttatt
     1621 gttaaaagtg ttgttgcaca aggccaatgc agtaaactga acagcataac atttaaagct
     1681 gtcaaataag tccatccatg ttctctaaga acttgaccta aagcactaaa gctatctaat
     1741 tctatcatag aaccagttgc taggtaagcc attaaaagta taggtacaac tatctcattt
     1801 gctgggaatc caagaataaa agcaagtaat ataaaaccat caagaccaat taatttagct
     1861 aaagggtcta aaaagtttgc aacatgtgat aatatactta aatctcctat atatatattt
     1921 gcaaatatcc aagttatgac accagcaggt atggctacca ttacagccct tcctaaaaca
     1981 aatattgttc tatctattat agaggtatat aaagttcttc ctatttgagg aactctataa
     2041 ggaggaagtt ctaatgtaaa tgtagacgga actcctttta atagcgtctt agaaagagtg
     2101 taagaaacca acaatgttat tataacacct agaattatca gtaaagttat gcaaagtgca
     2161 gttgctacgc ttgatacgaa agagttggtt ataactgagc taaaaaatat tgtagatata
     2221 gctatcaaag taggaaaacg accattacaa ggaacaaagt tgtttgtaag tatagctata
     2281 agtctttccc ttggagagtc aattatccta caaccaataa ctccagctgc attacatcca
     2341 aatcccatac acatagtcaa acactgtttt ccatgtgcac aagctttttt aaataggtgg
     2401 tctaagttaa atgcaactct tggcaaatat ccaaaatcct caagtagggt aaataatgga
     2461 aaaaatattg ccataggagg tagcattaca gagattaccc atgctaatgt tctgtataat
     2521 cctaaaacca acatatcatt taaccatgat ggacaattaa tactatttaa tatacccgaa
     2581 atacttggct caaatccgag taataaatta gataataaag tggatggata gttagctcct
     2641 tctatagtaa tccacaatat agtacctaat aataagagca ttattggaag cccaaatatt
     2701 ttagatgtta atattttatc tactttttct tctctatcag ttgatttttt agcaacatta
     2761 gaacaacact catcactcag ttttttagca taatcatagt tgattttagt aaactcatct
     2821 cttattttct gtttatttat attgtctgga atctttttct ttacttcatt aatagcatct
     2881 atagaatttt tgtctatata attactcata gattcaaata tactttcatc gccatcaata
     2941 agtctaagtc ccaaccatct agaatttata cctggtataa tattatccaa ttctggttga
     3001 attgatttta ccacattctc tatattttca ttgtatctaa caggtttatt atttaattta
     3061 tatttgtcaa aagatacatc atttaaagta tccaatagtt catccatacc agagccattt
     3121 cttgcagcag ttaaaataac tggaatacca agagaatcct ctaaaagttt tttatcaata
     3181 gtaatgcctt tttttctagc ttcatctatt aaattaatac acaaaataac tttatctgta
     3241 agttccatca cttgaaatac taaatttaaa tttctttcaa gacatgtagc atcacatact
     3301 actattactg catctggatt accaaaacat ataaaatcac gagctacaat ttcttcttgt
     3361 gataaggcaa ataaagaata tgttcctggt aaatctatta atgcatattc ggtattttta
     3421 tatttaaaat taccacgagc tgtagctaca gtttttcctg gccagtttcc agtatgctgt
     3481 ctaagtcctg taagatgatt aaatacagta cttttacctg tatttggatt gccagccaat
     3541 gcaattacaa actggtcttc cttattgtct atatcaaaca tgtcttttaa agaactcatt
     3601 ttagttgagt tatgtgtcaa ccccatatta atatcctcct gctagaaatt tttaaatgtc
     3661 agaaactaat attaagctac tttcttcttg tcgtagagct atcttgctac ctctgatttt
     3721 atagacagtt aaattatttt ttggaccttt tctaactaca tctataacag ctcctctagt
     3781 aagaccaagc gctaacattc tttctcttaa attaccacta gataatatat cttcaacttt
     3841 gacagttttt ttcacttgaa tatcgttaag gtttttcata atagcacctc catgcttgaa
     3901 atttagcctg ggctaatctt cttattttaa actatgcatt atattattac cttgttactt
     3961 ttacaaaaaa aagataggca agatgcctat catacaaagt tgggtataat tcttactttt
     4021 ggggaaagta gaatatagaa taaaaattaa cactttgaat ccatataatt attgaatttt
     4081 gataataaaa aagtcagaaa gcaaaagcct tctgactcta tgtaaggtat tttcaaatac
     4141 ctaaatatca attcaatatt taatttatac ttaaattata tattaacaat attagcttgt
     4201 caacactata atctcatttt aatatgttca atacctgctt cgtcgtatac ttctgagatt
     4261 tctttaaaac cacaagataa gtaaaggttc tttatataag tttgtgctga taaggttata
     4321 ttattttcgt gtaagttaac ttttataaaa tctatagcac aatccatcat ttgttttgct
     4381 aatccttttc ttctatgact agaaagaact aaaactctac caatggaaac atcattataa
     4441 gagctgtatt ctttaggaat tattctagcg taagcacaaa tcaatccatt ttcttttaaa
     4501 aatatgtgat aagagttttt atctatatca tcataatcgt ttaatgaaaa tatcttctgt
     4561 tcacaagcaa atacctcata tcttgatttt gctatttcat aaaattcatc taaactcaaa
     4621 tcattaaagt gtttaatttt aaacatagta attacctcct agcatcttaa aaaactgaat
     4681 attaagatac attataccat ttttaacata aataccttta ttaaaattta tatttattaa
     4741 aaatattttt aaaaagtgat atactatatt aaaaacaaaa ctttagttag aaaatttatg
     4801 atttatggag gcagtgtttt gaatactact aaaaaattaa ctgaagcagc tttattgtca
     4861 tctttattca tagtagtaac aattatagct gtgagtactg gatttggata tgctatttac
     4921 ttagatttta tagtgccagt atttttttgt ataatctgct tgaaatgtga tttaaagtat
     4981 accattttat ctggaattac ttcgctttta ataatttctc tggtacttgg aaatctagga
     5041 actgctattt gggcaagtca gagtgtatta cttggtataa tgtgtggcta tctaataaat
     5101 aagcctacaa tggttatgga tgatttagta tatggttcag tatttgggct tattgtaatg
     5161 gtttttattg atatatatgc ttcaacatta ataggttata gttttatgaa agaatttcaa
     5221 ggatatagca aatggattta ttttaatggg tatacaaatt ttatttatta tttaatgata
     5281 gcattgtttc cttttggtat ggtgttttgc atatatttat taagtttaat tttagggaat
     5341 aaattgcata ttttagattc taattctcta aaaaaatttt taatatttaa gaattttaga
     5401 agtttaaacc aatttttatg ttgctcaaaa aaagcttttt acatatgtgt ttcttatcta
     5461 gccatttttg aagttctaaa actgttgaat gttaaagttg attctgttta tttaaaaaca
     5521 atctttatat caataaccta tataagtctc tattttatca ttagagactc atgtatgtca
     5581 ctacaaaact ttataatagc taagtttaga aaattattat atgctagaat tttatttttg
     5641 ataataattt tattgctgtt ttttatattt gatgtaacag ttattggttt aataattata
     5701 aacgcatttt taaataagaa aataaatata agattgagtc agagtaatat agtcaataaa
     5761 caaataaatc ttttgattga aaaataaatt ttgggtattg gtaaaaaata taaaaaagta
     5821 gtataatgta aaaaaacatg tatataataa taaaaaaggt tgacaaaata taaaaataat
     5881 aatatattat gagtaagtta agaaaaattt cttgtaattg atgaaataat ctgaatatat
     5941 aaatgctatg aagagaagaa gtagggctag attttattta acagagagtt ccagtttggt
     6001 gaaatggaat aaattgattt agtttgaata catctcagag cactatctct gaaagataac
     6061 aaccattaag agatatcgga ttagtacacg ttatagtaca cgagtatatg taattgtact
     6121 ctaagagatt aatatcgtga gatattgatg aactaaggtg gtaacacgta agcaatgctt
     6181 tcgtcctttt aagaggatga aagctttttt tattataaaa ataaaaatta tagattatat
     6241 aaatatttta ggaggaatga aaaatgaaat cgatagacga gcaaatgcga ataattatga
     6301 aaggtgtaga tgatttaata gatgagaaag agttaaggga aaagcttatt aaatcagaaa
     6361 aagaaggaaa gcctatgata gtaaaattgg gtctagaccc aagtgctcca gatatacatt
     6421 tagggcatac agtagttctt agaaagatga aacaattaca agatttaggt catcaaatag
     6481 taataataat tggagatttt acaggaaaaa ttggagaccc aactggaaaa tctaaagcta
     6541 gaaaagcttt aacaacagag caagttcttg caaatgcaaa aacttatgaa gaacaaattt
     6601 tcaaagtttt agataaagaa aaaactattg taagatttaa tagcgagtgg ttagctaagt
     6661 taaattttga agatgtaata aaattagcag caactattac tgttgcgaga atgttagaaa
     6721 gagaagattt taaaaagaga tatgaaggac aaatgccaat atcagtacat gagttcttct
     6781 acccattaat gcaagcatat gactctatag cattagaagc tgatatagaa cttggtggaa
     6841 ctgaccaaag attcaattta ctaatgggaa gatcattaca aagagaattt ggtatggagt
     6901 ctcaaatagt aataatgatg cctttaatag aaggattaga tggtaaagaa aaaatgagta
     6961 agagtttagg taactatatt ggaatagatg aagaagcggg aataatgtac caaaaatcta
     7021 tggaaattcc tgatgaactt ataattaaat actacaattt agtgacagat gttcatcctg
     7081 atgaagttaa taaaatagaa tcacagttga aagagggttc agtaaatcca agagatataa
     7141 agatgaactt agcaagagaa atagtaacat tataccatgg agaagaatct gctaaagaag
     7201 ctgaagaaag atttaaatct gtattccaaa aaggtcaaat acctgaagat atacaaacaa
     7261 ttcaagttaa ggaagatgga tttgacttaa tcgaagtact agtttctaat gagattgtta
     7321 agagtaagag tgaagttaga agattagctt ctcaaggtgg agtaaaggta aatggtgaaa
     7381 aagtagagga tttaagcact attgtaaaag agagtgaatt agttgttcaa ataggaaaga
     7441 aaaaatttgt aaaaatcgag ctagtaaaat agagtaaagt aaaagcaagt ctattgagta
     7501 taaaatgtac attcaataaa cttgcttttt tattatatta aaattttaaa ttgataacca
     7561 ttgtctttaa aatatgttat aatttggtcc aatgcattta ctgtttcttg ttttccataa
     7621 gtatcatgca ttaaaagaac aaccatttct ttgccttcag aagatttctt tgcatgttca
     7681 aataattctt gtgcattttt ctttttacct tctgcatcag catttaaagc gttccaatca
     7741 attgatgcca tgtttttctc ttttaagtag tttcctagag gttccatatt tttccatgac
     7801 atatacccgc ctgggcatct aactacattt gaggaaaaat tctttcctaa gacttttttc
     7861 atagcttcat cagttttgtt aagttcattt acgaatgtat cgagatttag gcttctgtta
     7921 ggatatagtt ttttatagtc atgagtataa gagtgatggg ctattgcatt tccttcgtca
     7981 aatgttcttt ttaaaatttc attagctcct tttctttcaa gagaatcacc cttaataaag
     8041 aaagttcctc taacaccatg tcttttcaag atatctagaa cttgtggagt attagtagta
     8101 gatggtccat catcaaatgt aagaaagact atcttttctc cattgtttga atagtcgtat
     8161 ttgttaagtt tgtcccaaac tatttttgcg tcatatccat attcttttac agcttctttt
     8221 tttgcaccaa taataggttc atttttttct ttttcaattc tttctttttc taatctttca
     8281 gctttaagtc tttcttgagt ttctttcatt tctttttttc tagacatgtc cgcccatgcc
     8341 atagaaaaaa taccaataac tattacaaca agtatagtaa gtgtaacagt tttctttttt
     8401 ctttttttta ccataattac agcattcccc cttttgtcga attgtatcaa aaatatatta
     8461 taattatatc gaagaaagta gaaaaagtaa accgacaaag ttgtaactaa gataactaaa
     8521 gtgtaactta tatgacatta tttgcataat attaaagtat catatatatt aaaaatatct
     8581 ataaaaaaat ttagtatagg taaaaaaata taaaaaatat aaaaatttat taaaaaggtg
     8641 tttacaaaaa atgattaata tattactgtt aaatagtgga tataaatttg agctcaaaat
     8701 gtatgtaaat ttagatttaa aatactgaaa aaatttacta tattaattta tagtaatgct
     8761 tatagaaaaa ctctcttgct gtttaggggg tttataattt tgcaaaaaaa taaaaaggag
     8821 ggggctttat tgaaaaaata ctttggagaa ataggactaa tatttatagc aattatatgg
     8881 ggaagtggct ttgtagcaac ccaatttgca ttagacggtg ggcttacacc tctacaaatc
     8941 ataactttaa gatttttctt ggcagctata attatgaatt tactgttttt taaacagatt
     9001 cgtgctaata tgggtaaaaa attattaaaa gcaggaggga ttttaggaat atttttattt
     9061 ctagcattca cagtacaaac tattggactt atgtacacta caccatctaa aaatgcgttt
     9121 ataacagcag ctaatgttgt aatagttcca ttcataggat ttatacttta tagaagaaaa
     9181 cttgataaaa taggaataat aagtagtttg gtagctctta taggaattgg gatactttct
     9241 ttagaagctg atttctctat taattttgga gattttctaa cacttatatg ttcattcgga
     9301 tttgcatttc atatattctt tacaagtgag tttgcaaaag ataacaaccc aatggcattg
     9361 acagctattc aatttacagt agcgttttta atgtctgttg tagttcaaac atttgcaggt
     9421 caattaaaga tggaagctga attatctggt tacatgggaa ctatgtatct agctgtattt
     9481 agtactacaa ttggattttt atttcagact atatgtcaaa aaagagttga tggaactaga
     9541 acagctataa tattatccac agaggctgta tttggtacaa tattttctat aataatttta
     9601 aaggagctta ttacagcaaa attggttatt ggtagtatct tgatatttgt tgcaatcata
     9661 actgcagaaa ctaaattatc ttttttaaag agcaaaaaag tgaaacttaa agattcagag
     9721 gagtcttcat tagaaagtat ttaatatgac aactatttcc taattaatca aataaattat
     9781 gtattatgta gaaatttgac atcaaaactt ggttgatact ggtgtttcaa agtgataaaa
     9841 ttaattagga aataaatttt atttttagtg tttaagctat aaagtctggg gtatatatat
     9901 tcttgtagca agttattaaa cttaaaaata aaaatttaat tatataaaat tgggaggaaa
     9961 ttaattatga aaaaatttgt ttgtacagta tgtggatata tacatgaagg agatgctgca
    10021 ccagcacaat gtccagtatg taaagttgga gctgataaat ttgaagaaat gaaaggcgaa
    10081 atggtttggg ctgatgaaca tagaatagga gtagctcaag gtgtagatgc agaaataatc
    10141 gaaggattaa gagccaactt tactggtgag tgtacagaag taggaatgta tttagcaatg
    10201 agtagacaag ctgatagaga aggttatcca gaagtagctg aagcgtataa gagaatagct
    10261 tttgaagagg ctgaacatgc tgctaaattt gcagaacttc ttggagaagt tgtagttgca
    10321 gatacaaaag aaaacttaag agttagagtt gatgctgagt atggtgcaac tgatggaaaa
    10381 ttaaaattag ctaagagagc taaagaatta ggattagatg ctatacatga tacagtacat
    10441 gaaatgtgta aagacgaagc tagacatggt aaagcattct taggattatt aaacagacat
    10501 tttggaaaat aataattaaa acatagatta aaaggagaaa ctttggtttc tcctttttta
    10561 aattatacaa ataaactgtt gtgatagaag gatggagata atatgaagtc ggtatctgtt
    10621 aggagttgta aaacttacaa ttatgaagaa gtaaagttat ctattgaaaa aaatttagag
    10681 gatattggtg gtatagataa gtttgttaaa aaaggaagca aagtacttat aaaaccaaat
    10741 ttattaatga aaaaaaagcc tgaagaagct acaactacac atcctatggt tgtaaaagtt
    10801 gtatgtgaaa aacttttaga gttaaattgt gatattacaa tagcagatag ccctggtggt
    10861 ccatataatc aaagttctct taagagcatt tataaggcat ctggaattga agaagtagca
    10921 aatgaactgg gcattaagtt aaattatgat gtttcagatg ttaagataca tagtgaaaaa
    10981 gcacatgttc taaaatatat ggatattatt aaaccaatag tagattcaga ttatataata
    11041 aatctttgta aattaaagac tcatataatg gcaactttta ctggtggtac aaaaaactta
    11101 tatggatgca tagctggatt gaaaaaagca gagatacatt atagatttcc tacagaagag
    11161 ttgttttgcg aaaatgtact tttagatatt tgtgattata taaaaccaac attgacaatt
    11221 atggatggta tagtaggtat ggaaggtgat ggaccttcag ctggtactac tcgagaaata
    11281 ggcgttttat tagcatcaca aaatccatat gctatagatg ttgttgcatg taagattata
    11341 agtttacttc caaatagagt tgctacaatt agaggtgcta ttaagagagg atatatacaa
    11401 gaagattttt cagatattga aattttaggt gaagatataa aagatttaat tataaaaaat
    11461 tacaagattc caaatgtaag taaggattta agattgtttt ctgtaaagct accaaaattt
    11521 ttaaatgaac ctatttcaaa gcttataaca ccaaagcctg tggtaagatt tcaagattgc
    11581 ataaaatgtg gaaaatgcaa ggaagcttgt cctgcatcca ctatagagat agggactaag
    11641 ggtgctatta tagacttaag taaatgtata agatgttact gttgccatga actttgtcct
    11701 aaaaaggcta ttgatgtcaa acgtaatttt gtatttaagt atgtaaaata atgtataata
    11761 ttatatgagt ctatataaat taaaaatata aaggagaaat aatatgattt taattaaaaa
    11821 tggtagagta atagacccta aaagtcaaag agatgagaat attgacttaa ttataaaaga
    11881 aaataaaatt tataaaatag gcaagtttga tgaaagtgat gaatatgaaa aaataataga
    11941 tgctagtggt aatgtagttg ctccaggttt agtggatgta catgtacact ttagagaccc
    12001 tgggtttaca tataaagaag atatagaaag tggagctaaa tctgctgcta gaggtggatt
    12061 tacaactgtt atatgtatgg caaatactaa tcctattgta gataatgaag atacttttaa
    12121 ttatgttaaa gaaaagtcaa aaaatgcatg tataaatgtt ttacaagcag cagctataac
    12181 taaaggtttt gaaggtaaag agttagttga tatggaagct cttaaaaaag ctggtgtacc
    12241 agggtttact gatgatggat tacctttaat ggatagcaat ctaattatgg aggcaatgat
    12301 taaggcaaaa gagttggatg taccacttag ttttcatgaa gaagaccctt ctttagttgg
    12361 aaatccaggt gtaaatgcag gaaaggtagc ttcagaactt ggattaaaag gtgcatcaag
    12421 tgtagcagaa gatgttatgg tagccagaga ttgtatgttg gcactaaaga ctggtgctaa
    12481 agttgatata caacatataa gttcaggtgt atctgttgat atggttagat ttgcaaaata
    12541 cttaggtgca aatgtagttg cagaagcttc accacatcac tttacattga ctgaggaaga
    12601 tgtattggag tttggaacaa atgcaaaaat gaatccacct ctaagaagta aatgggacag
    12661 agataagatt atagagggat taaatgatgg tactattgaa ataatagcaa cggaccatgc
    12721 acctcatagt aaagaagaaa aagatagaga gtttataaaa gctccaagtg gaattattgg
    12781 gcttgaaact tctttagctc ttggaataac aaatttggtg cataaaaatc atcttagtat
    12841 gatgcagtta atagaaaaaa tgtctataaa tcctgcaaag ttgtacaatt taaatattgg
    12901 atttatagaa gaaggggctg tagcagacat tgttatattc aatccagaag aagaatggac
    12961 agttgaaagc tttgtttcta aagctgataa ttcaccattt aaaggtaaat ctttatatgg
    13021 aaaggttaat tatacaatat gtaatgggga gattgtatat aacgcctaat ttaagattat
    13081 tttttttagg cggagtgtac aatgattgaa agtaaaagat tgtatattag gagaatgaga
    13141 ttgattaatc aaaattggaa gtattagttt gaacaattag aacataacat cttggtaatt
    13201 tagatatagt aaggaagaga atccttttta tgaaagaacg cactattata tttggaaaga
    13261 ttaagttcct aatctatcat atataatagt gcgttatata tttaagtatg ttttatataa
    13321 actaatctaa aaatcgaaaa tctttataca atttctactt ataaattgcc tttatagggt
    13381 ctagtttaga ggctttaaag gctggaatca gaccaaatac aactccagtc aatatagtag
    13441 ctacaattgc ataaaataag ctatttaagc taggtatagc ttcaaaacct atatatttag
    13501 aaacatagtt agtagcagta aatccaacta tagttcctaa aattccacca catactgtta
    13561 taaaaacagc ttcaactaaa aattgaaata atatagacct aggttttgct ccaatagcac
    13621 gtcttatacc aatttctctt tgtctttcca taacagatac atacattata ttcattacac
    13681 caataccacc aacaaacatt gcaactacag ttattataga tacatatttg tttacattgg
    13741 aattaatgct ttcaagataa gctgtctgtt cagttgggtc tggtgtagta taagaaccat
    13801 tgataccagg gtgcatctca tataatttag ctataacatt gtttgcaact tcatttacat
    13861 tatagccttt agaagaaact aagtctaact ggtaaatttc atttgaataa gaattttgac
    13921 tcattaaagt gtcaaaagcc tttttaggta ctagggaagt agtaaattgc atatcttggt
    13981 aacctccaaa gaatcctcca gcttggtttt gctgagaatc atctaatacc cctattattt
    14041 caaatatagt tccatttatg ttgataccat gacctaaggc atcttctgga ttttcaaaaa
    14101 tttcacttgt actttgtaat gtaagtaaaa ttacttttct tttttcatca tctaaagaaa
    14161 aatctctacc acaaattaaa tttattttag aatctttttt tactggtcca acatcaacat
    14221 acgtagtttt tttatcaaaa gatgcttgtg aagagtaaac tgaatcaagg ttaaacccat
    14281 ctcttgaagg agctattctt tctactcctt caacaaatga taattcttct aaatcttttg
    14341 cattaaatgg ttttaaaaaa atactcatat catcagtaag acctgtgtta tcggctgatt
    14401 caaacgaaat agtagtttta ttagggttta cattgtttac agatttctta atttcttttt
    14461 gaaatccatt acctatagaa ctaacaagta ttactgaagt tattcctatt ataatccaga
    14521 gtaaagctac aaatacacga agcttgtgac cttttaaatt ggccaaagaa ttttttaata
    14581 aactcatgat tacacctccg aagtaaacac accatcttta agtcgtataa ccttagttgc
    14641 atacttagtt aaatcttgat catgtgtaac cattattata gtcttacctt gtttgttgag
    14701 ccttgttaaa agctccataa tttctctgct agtttcacta tctaatgccc cagtcggttc
    14761 gtcagcaaag attatctgag ggtcatttgc taggcaacgt gcaatggcta cacgctgttg
    14821 ttgaccacca gataattgaa gtggtttctg cttaagcttg tccaaaagcc caacagaact
    14881 taatttatct ttaacaattt cttctctatt agattttttt agtgctttat tgtaaattag
    14941 aggcaactct acattttgat atacatttag agtttctatt aaattaaatt gttggaaaac
    15001 aaatccaata ttaatatttc taaaaacaga acgttcattt tcactcaaat tagttacatc
    15061 tgttccatca aatatatatc ttccttcatc aaatacatct agaaaaccta agatattaag
    15121 taaagtagtt ttaccactac cagacttacc cattatcatg acaaattctc cagattcaat
    15181 ttctaaattt aaggatttta aaacatgtaa ttcatctttc cctactttgt aatatttttg
    15241 aatattttct aatttaatta acatatgtaa attaattcac atccatagta acttcttcta
    15301 cttttttgtt tggagtagta tctccattat tagaaccagc agttacgcca tcgccttgaa
    15361 ctgggtctcc ttctttcatt tctttagtag ggtgttttat tattatatcg ccttgttcaa
    15421 gaccgcctct aactacagca aaatcatcat tttgacttgc tacatctaca atttgctttt
    15481 tcaatatacc atctaaatcc ttaaatacat atgagtgttt gttgtcttta agtacacaag
    15541 atgctggtat tttaaagctt gaattagcta cttcaagtga tgcttgaaga tggaatccat
    15601 ttactaatcc ttcttgatta tcaaatgcta tgtttatatc ataataagat agtgtattct
    15661 gctgagctcc catttcagta tttggagtag atggtttatc agatataaaa cttattctac
    15721 cagtaagctt ttggtcagtt gaaaaaacta gtatattgac catttggtca atcttcattt
    15781 taggtaaatc ttgttcactt gcttgtcctt tcatataaaa ctctagacct tgaatagtca
    15841 taaatgaaac tggttggtct ggatttccag tttggtcatt caagtatact gtgccatcaa
    15901 aaggagcagt aactctgtca taagcttttt tatccagaga agctatctgg gagcttaaag
    15961 cagttatctg tgcatttata gaagcaatag catctttatt ttcagcaata tcagaaagtg
    16021 atattttttg ttttttgtat tgactaagtt gtgattttaa actatctatt tctgctatta
    16081 tagttggatt tttagcagta aaaagaaggt ctccttgatt tactttttga ccatttgtaa
    16141 cattaacatc acttaactca taatctccag atatattgaa gtccttagtt tgcttaggaa
    16201 caaccatccc atttataaat attttttcat tttcaggaat agtatatgtt tcgataaaat
    16261 tttcattagc ttgtaattca gtgtttttcg aatcaacata agcaagaact cctagaatta
    16321 aaagtatgat tactgctatt cctgcatata ttttgaatct cttattttta aagggattgt
    16381 ttttaaaacc tttattttta gacttgaagc cactacttcc aaaactgcta tttctaaagt
    16441 tgttactttt aaaactgtta tttctaaatc ttttattttt aaattttttt agaaaagaca
    16501 tgtcttcctc ctttttataa aaactactta attatatact gggatgtaca tatagaatta
    16561 ctataagaag ttgaatataa gttaatgaaa ataaactatc aataataaga tactataaat
    16621 cacatttgaa tgtaatagta aattttgtat attggttgta ctctgaatct acatcaattt
    16681 tatgaccaag cttatcacaa agttctttta ctaaataaag cccaagacct gtagatttag
    16741 aattgtttct tccattatta cctgtaaatc ccttattgaa tattcgattt aaatcttcct
    16801 tttttatacc acaaccagta tcttccactc ttaattgtaa ataatttttg gttcttttgc
    16861 aatagataga tatattatct cctaatgagg agtattttag ggaattactc aataattgct
    16921 cgaatatgaa gtatagccat ttttcatcag ataaaacctt aaaattcaaa ttatttaagt
    16981 ctaaaataat tttttttgaa ataaaatatt ttgagtattt ttttataata tttctaatga
    17041 ttttttcaat atttacctct tgaattaaaa aatccttatg aaaatcttcg agtcttgata
    17101 aatataaaat tttttcaacg gagttttcaa ttctaaatat ttcactatct atgtcatctg
    17161 aaattttttc atcaattaaa ttttcatttt gttcaagaat gagttttatt atagctatag
    17221 gtatctttac atcatgtacc cacatattta aaatctcttt gttttcttca aatttctcga
    17281 tattattatt tattatgtta ttacattgat attgatgatt tttaataatg ttcaagtata
    17341 ttttttcttc atttttacat ctagctaatt ctatatcatt catattaatt ttgaatgtat
    17401 cattttttat cttatctaaa aatttgttct tcttgatgta gctaataaat agatataaaa
    17461 ataaaaacac aacatttaca actaatatat ataaaatagt atcagtcaga aaaacatcta
    17521 caggagaaaa tataataact gatactgtaa aaattaagaa agtaaaattg tatactaaaa
    17581 aacctagttt atccttgaga aaatctttaa agctcataaa ttcacctact tagaattgtt
    17641 ttaactgatt atataaccca atcctctttt tgtttctata aaatcttcta aaccaatttc
    17701 atttaacttt gagcgtattc tatttacatt gacagttaat gtattatcat ctacaaacca
    17761 atcattatcc cataattttt tcatgagttt attcctactt actatctgcc ccttatattt
    17821 cataagctca tgaagaattt tgatttcatt ttttgttagc tcaatagtat tatcttcata
    17881 gtttattgtt gcagttgata aatctaaagt aacaccattg tgaataatct gatttgagtt
    17941 attatcaaca aaattataag ttcttcttaa aagggcatta actttagcta ctaagacatc
    18001 tacagaaaaa ggtttttgta tataatcatc tgcacctaaa tttacaccca ttattacatc
    18061 catattagaa tttcttgaag aaataaaaat tataggaact tttgaaattt ttcttatttc
    18121 attacaccaa tgaaaaccat cataaaatgg aaggtttatg tccaataaaa ctaattctgg
    18181 gttatagttt ttgaactctt ctattatatt ttcaagattt tctatttgat gaacatcatg
    18241 tccccattta gtaagacact ttgctatgat atttttcaat gagacgtcat cttcaactac
    18301 cattatttta aacattctta cctcctaatt caaaatagtt atattaacat aactatattc
    18361 tacttgataa attaaaatat ttctacctta ttacctaaaa taattatatt aatataacta
    18421 tattttactt gataaattat attttaaaca taattgcaat aaatttgaag aaatataatt
    18481 aatacttacg aaaatgtaac cttaaattaa tataattaaa tgtttaaaag ccattttaat
    18541 atatataata tatttgtgag aattgataaa taactaattg tgatgaaagg tgattattaa
    18601 ctatggaaga aatattatct gttgaaaata taaaaaagga gtatggaaga aaaggttcaa
    18661 aacatgaagc tctaagagga ataactttca aggtttataa aggagaattt gttggtatta
    18721 tggggtcttc cggtgctggt aaatcaacac ttttaaatat tatttctaca atagatttac
    18781 cttcatcagg agatatttat ataaatggaa agaatactat taaaatgaag caaaatgaat
    18841 tggcagattt tagacgtgat aatctaggct ttgtatttca agattctaat cttttggata
    18901 cacttacaat aaaagaaaat ataatgttgc ctctttcttt aaaaaatgaa agagtatcag
    18961 taatagaaaa tagaataaaa gagatttcaa aagaattaaa tatagaatct atattagata
    19021 aataccctgg cgaagtttct ggtggacaaa agcaaagagg agctgtatgt agagctatag
    19081 caacaaaacc atcattagta cttgcagatg agcctacagg agcattagat tctaaatctg
    19141 ctagggattt gttgaattgt ctattaaaat taaacaaaga cagcaataaa acaatattga
    19201 tggtaactca tgatgctata agtgcgagtt tttgcaatag aatactcttt ataaaggatg
    19261 gaataatatt cacagaaatt gtaaagggag aaagtaacag agagttctat aataaaatag
    19321 taaatacagt ttccttaatt ggaggagtta ataaaaatga ctttatttag aattgcaatg
    19381 caaaatgtaa agaatagttt ttttaattat ttaatgtact ttatttcaat tgtatttagt
    19441 gtatttgtat tttttagttt taaatctata gagtataatg aagcgttgag ttcacttgga
    19501 gagaaaacta ggatgagtat taatacaagt tcaattgtaa tagtggtatt tgtattttta
    19561 tttatatatt attcaaactc attttttttc aatagacgta ggcgagaagt aggaacttat
    19621 agtttacttg gaatgaggaa aaatcagata ggaaaaatat ttttatatga aacttttttg
    19681 atgggtatag ttgcaatact aataggtata tttttaggac tgttattttc aaagcttatg
    19741 actatgatgc ttgtaaaatt aatgaatgaa atgatagtag taaatatgaa cttaagtata
    19801 aaggccttag ttcagacact agcggtattt ttaataatat tcatagttat tggaataaga
    19861 aatactatag ttatcagaaa taagagaatt atagagttat ttaataaata tccagaaaag
    19921 tataagttta aaaaatttat aaagttaaaa tgtgtacttg gtgttttatt aatagctatt
    19981 tcttatttaa tgtcaattag ttattttatt gctgaaaata ttatgctttc agtttttata
    20041 ttagttacaa taatacctgg aactttttta tttttttcat caacgatgta tatgattatt
    20101 gatgctgtta agaagagaaa aagtttttat tataagggac agaatttaat tgcattttct
    20161 gaacttggat ttaaattaaa gagcaatagt agtgtattgg ctgtaatagc aatattaata
    20221 gctacaagtg taactatgct agggtttacc atatcacttt attacgatat agataggaac
    20281 ataagtgaaa attataaata cagttatacc ataaatttag gaaattctta tgtaaataac
    20341 gaaattgaca aattattata taaatataaa aaagataata agataatctt tgataaaaca
    20401 atagaactta tagacaaaga tataaatatt gaaatgtatt ataaaaatgg aaatagttat
    20461 atagaagata gaacgtctgt agatattatt agagaaagtg attttaaaaa attaagacaa
    20521 tatcagaata acaattatga agaattaact tcaaaggaat ctgtgtacta tgtttctgat
    20581 tcatacaaaa aaatattttt taaagacttt gaggttgaaa aaataaactt gcatattaaa
    20641 gatacaaatg agtatgatat aaatctgttt aaagtacaaa aaagcatctt ggagccagaa
    20701 ataaattcac aagctacgtt tgatttaatt gtagcaaaag atgacgtatt taatcagtta
    20761 aaattacatg gaaattctag attaataaga ttaattgata taaaaaatga aaaaaaagaa
    20821 ctggatttga ctttaaaatt aaaaaatata gtaagtgaaa atatgaaatt tacttatcct
    20881 tttaatttta catcaagtat tgaaagttat aataacttga taaaattaag tggtctaatg
    20941 ctgtttatag gcatattttt gtctgtagta tttcttttat gtacaggtag tataatctta
    21001 tttaaacaat tatcaaatat atatgatgat aaggaaagat atattatgtt aataaaactt
    21061 ggagcaaata ataaagatat tgagaaaata atatcaaaac agttaaaagt tatattttta
    21121 atgccattag ttgtaggtac agttcataat ttatttgcta tgtctatagc acaaaagttt
    21181 ataccaaggt cattacttgt accaattatc ataactctag tcatttattt tataggatat
    21241 tttatatatt attttttaac tttaaaatat gctctaaaca tgataaaaga ataaattatt
    21301 tttaagtcaa aagaattaga tatatgtttt gaataaaata acttatttaa gtgcagatag
    21361 aaaatactag aattgagttt aagatattta tttaaagaca aaattaaagt taggaaaaag
    21421 tctatatttt atatcatttt gtatcagtta cattacttga tgttatataa taaatctaag
    21481 cagaatatca aataaaaaat gacgaggagc tgaaatttat gaagtttaat gcctaccaac
    21541 ataaatactc atcaataaga aatgtcgtgt atgctaaaaa tggagcagtt gcaacatcaa
    21601 caccattagc atctcaagct ggacttgaaa tactaaaaaa gggtggaaat gctgttgatg
    21661 cagcagttgc aacagccgcc acattagcag tagtagaacc tacaagcaat gggattggag
    21721 gagacgcata tgcccttgtt tggatagagg aagaaaaacg attgtatggt ttaaattcta
    21781 gtggctttgc tccagaaaat atggaattaa aaaattataa taatatgaaa gaaatgccaa
    21841 aatatgggtt tggagcagta actgtacctg gaataccagc agcttggtca gaattaaata
    21901 aaaaatatgg taaacttagc ttaatggaat gcttatctcc agcgataaac tatgcaagag
    21961 agggatacgt agtatctcct aatgtagcta aggtatggaa aaaatcatat gaactctatg
    22021 aaaaagaact tattggagaa gagttcaaac catggtttga tacattttct aaagatggta
    22081 aagctccaga ggctggagat atatttatat gtgaagaaca ggcaagtaca ttagaagaga
    22141 tagcaaatac tcaggctgaa agcttttata gaggaagact tgctgataaa atagatgaat
    22201 attctaaaaa atttaatggg gctataagaa agagtgactt agagtgtttt tatccaacat
    22261 gggtagaacc tataagtaca gaatataagg gatataaaat atttgaaatc ccaccaaatg
    22321 gtcatggaat aacagtttta atggcactta acatattaaa agaattggaa ttagaaggaa
    22381 atatagaaaa tgtagaagac atacataaaa ttatagagag tttaaaatta gcttttgcag
    22441 attctaaaac atatgtaact gatatagagc atatgaaggt aaagattcag gaattactaa
    22501 gccaagagta tgctaaaaaa agaagtttat tgattgacaa taaagaggct ttgtatccaa
    22561 ctgcaggaga gccatattgt ggtggtacag tatacctttg tactgcagat aaggatggaa
    22621 atatggtttc atatatacag agcaattaca taaattttgg ttcaggaatt gtgataccaa
    22681 gaactgggat tgctttacat agtagaggaa ataattttaa tttagaccct aaacaccaca
    22741 atgtagtaaa accatttaaa aaaccttatc atactataat acctggattt ttaggtaaag
    22801 aagataaagc aataggtcca tttggtgtaa tgggagcttt tatgcaacca caaggacata
    22861 ttcaagtgtt aacaaatatg attgattttg gattaaatcc acaagaggcg ctagatgctc
    22921 caagatggca gtggataaaa ggaaaagaaa tagaagttga accagagatg ccaaagcata
    22981 taatagattc attaattgaa aagggtcatg aaataaaagt aattcatgat actgtcgaca
    23041 tgggacgagg tcaaataata tttaaaacag aacaggaaag ttatatttgt gggacggaat
    23101 caagatgtga tggtcatatt gcagtttatt aatattatca actaaaatat atataataaa
    23161 tttaaagagt atatgaaata ataagtaaag gcgaaagtct acaatgactg acgcctttac
    23221 aaaatttaag ctatcatatg ctgattttat atagattatg agccttattc ttgaccagtt
    23281 ttgatttcat tgtcatcata actaatccag tcactaaagc taccaatata aatcttgtga
    23341 ggtatatcca gttctctaag agcaagactg ttaacacaag cagatatccc tgaaccacaa
    23401 gataaaatta cttcatcata attatttaaa tctttaaaga atttttttaa aaattctata
    23461 tctttaagtg agccattttc aaagtcactt tttattaaat ccttacaaaa ataatttttt
    23521 gcacttggta tatgaccagc ttttgagtaa gcaggttcaa ctaaaccctg gtatctctca
    23581 ttagcacggc aatctattat tacagtatct tttttgtata atttagattt aacatattcc
    23641 attggaacaa ctaaatcatt gtttatattt ggtctaattt ccttacctgt acaacttggt
    23701 atattcactt tctcttccaa ttctccacct tcctttacga aagatgttat tcccccatct
    23761 agtacataga catttttaag ccccaaatgt tttaattgga aaaataatct gcaagctcca
    23821 tttaaatctc catcatcata agttacaatc gtagtgtcat tatcaacacc cattttttct
    23881 aatttttctt ttagtattaa agggtcttgt aaaggatttt taccaccatg ttcttttgta
    23941 ggagatgata aatccttgtc tatatctaag ataaaactgc ctttgatgtg tcctttttta
    24001 taagaatcaa taccataagt tctattgata agatcaaatc tacaatctat tacaactaga
    24061 ttatcatttt tttctagttt tgatataagt tcttttgctg aaataatgtt tttcaaagaa
    24121 tgtacctcct gtgtttatat tatctttatt ttaatttata ttaattatta actttataaa
    24181 ctaatattat tataatttat tacataagta aataaatctt tagaaattgt aaaaatttta
    24241 tataaaaatt caaataatta atgaatattg aaaattttag atatataata aaataagagt
    24301 atattttata caatatgaaa gctggtattg caatagatta aagccttata cacgtttagt
    24361 ttttaaagaa attagtgtta ttatgaaaac ctaagttaat ttattaaata aataattgaa
    24421 taaaaaaatt ttattatgta tttataaaat ttttgttaca aatttggcat aatatagtat
    24481 actttaagtg tgtattaggt taaatctata acaaattaga cgtatgatat agacttagtg
    24541 tttattggca tttacaagaa atttgttaat atcaactatt tgtagtttgg gcagttataa
    24601 ttattatagg ggggatgtcc aatacaaaac aatcaaccaa gaactaatat tatattataa
    24661 agagttatag ttttgtgtgt ttatgttttt aattaaattt aaagtgggaa aaatatattt
    24721 gttagaatgg agaatagcga gatgagtaat aaaatagtta gtaagagaca attaacagat
    24781 agtatttatt taatggaaat agaggcacct agagttgcaa aatcttctca accaggtcaa
    24841 tttataataa ttaaaaatga tgaaaaagga gagaggattc ctcttacaat agctgactat
    24901 gatagagaaa aaggaacagt aactattgta ttccaaacag ttggtgcttc aactaaaaag
    24961 ttagcaatgt ttgaagaaaa cgattttgta atggattttg tagggccatt aggacaagca
    25021 agtgaattta tacatgaaga tatagaagaa ttaagaaata aaaagatatt atttgtagct
    25081 ggtggagttg gttctgctcc agtttatcca caagttaaat ggtttaaaga gcatggatta
    25141 gatgtagatg taataattgg tgcaagaact aaagaattaa ttatattaga agatgatatg
    25201 aaaaaagtag ctaaaaatgt atatgtatca acagatgatg gtacatatgg atttaatgga
    25261 agagttacag acctattaaa agatttagtt gataatcaag gtaaaaaata cgaccaagca
    25321 atagtaattg gaccaatgat aatgatgaaa tttatgtgcc aattaactaa agaactaaat
    25381 atacctacta tagtaagttt aaatacaata atgatagatg gaacaggtat gtgtggtggt
    25441 tgtagagtta gtgttggaaa tgaaactaag tttgcatgtg tagatggtcc tgaatttgat
    25501 ggtcatttag ttgattttga tcaagcaatg agaagacagt ctatgtacaa gactcaagag
    25561 ggaagagcta tgctaaaact tgaagaaggc gatagtcatc atcatagtaa ttgtggatgt
    25621 ggaggtaaca aataatggat gctaaaaaag taaaagtacc agttagagaa caagaacctg
    25681 ctgtgagagc aacaaatttt gatgaagtat gcttagggta taataaagaa gaagctatgg
    25741 cagaagctaa tagatgttta gcttgtaaaa aaccaaaatg tgtgggtgga tgtccagtag
    25801 gaatcgacat accagggttt ataacaaaaa ttaaagaaga tgatatagaa ggtgctgcta
    25861 aagtaatagc taagagcagt tcattaccag ctgtttgtgg tagagtatgc ccacaagaaa
    25921 gtcaatgtga aggtgtatgt atacttggaa taaaatcaga tgctgtatca ataggtaaat
    25981 tagaaagatt tgtagcagat tggtcaaaag aaaatgacat aaatttatct gatacagagc
    26041 ctaagaaaaa tcaaaaagta gcagtaatag gaagtggtcc agcgggtcta gcttgtgctg
    26101 gtgatttagc taaaaaaggc tatgatgtaa caatctttga agcaatgcat gagccaggtg
    26161 gagttttaac ttacggaata cctgagttta gacttccaaa acaagctgtt gttcaacctg
    26221 aaatagacaa tataagaaaa ttaggtgtaa aaatagaaac taatgtaatt gttggtaaaa
    26281 caattacagt agatgaactt atagaagatg aaggatttga agctatattc ataggttcag
    26341 gagctggtct tccaatgttt atgaatatac caggggaaaa tgcaaatgga gtattttctg
    26401 caaatgagtt tttaactaga gtaaacttaa tgaaggctta tagagatgat tatgatacac
    26461 ctattagttc tggtaaaaaa gttgctgtag taggtggtgg aaatgttgct atggacgctg
    26521 ctagaactgc attaagatta ggttcagaat catatatagt ttatagaaga agtgaaaaag
    26581 agcttccagc aagagctgaa gaagttcatc atgcaaaaga agaaggcatt atatttaata
    26641 cattaacaaa tcctaaggaa attttagtag atgaaaatgg atatgttaaa ggtatggttt
    26701 gtataagaat ggaattagga gagccagatg attctggaag aagaagacct atagaaatag
    26761 aaggttctga atttgtactt gatgtagaca cagttataat gtcacttgga acaagcccaa
    26821 atccactaat atcttctaca acaaagagtc ttgatataaa taagaagaga tgcttaataa
    26881 cagatgaaaa tggtcagact tctaaagagg gtgtatttgc aggtggagat gctgttactg
    26941 gagcagcaac agtaatatct gcaatgggtg ctggtaaaac agcagcagca tcaatagatg
    27001 aatatttaaa agcaaaagtt aatgcttaat ttgtaaatta aagagagggt gtctcaaaat
    27061 gaacttttta gttcatgagg cactcttttt gtacaaaatc aaatatgttt taatcatttc
    27121 aacaatgaaa atttcattcc tgtagaaagt ctaactttta ggtcttagta caatctctat
    27181 tttgatgtag ccactttttt agatggttta ataatttatc agttattatt gtaatatttg
    27241 atactaagta acataacttt gtctaactat gataaaatag ttatacaata aaggtcttgt
    27301 aaggagtgaa taatgtgtgg ctaataaaat cagatttgtc aaatctaaca ttaaataata
    27361 gaaaaataaa ttgtgtaatt aatggtattt taatatgaaa aaaagagaaa aagtatttga
    27421 tgggctagtt tttaagcata tattaatctt attaatacta ttagttttga ttgatttaac
    27481 aagtaccatc atatatgcaa gtaactctat ggaaattact tctagaaata tgattgaggc
    27541 atcaaaaaga gaacttgaaa actatttaga agtaaacata tctttattaa aagctcttag
    27601 tcaagatgat agattttcta atggtgaaac atcactaatt gaaaagggaa aattattaag
    27661 tccatatcaa aaggaatata atctttttat gattggtata acagatacaa aaggaaatgc
    27721 ttcaagtaca tatagggaaa atgttaactc aataaaagat aagcctagtt ttgagaaagc
    27781 aattaaaaca aagcaagtag tagtttcaga tattgaagtc agtaatgtaa caggtgacaa
    27841 agtatttatc atatatgtac caattgtaaa aaataatgag atgattggga ccatttttgc
    27901 ttctttttat tttcaagatg taaataattt aataagtaga agcaattttg atgattctat
    27961 taaatttcta atgatagata aagattatac tattatttct catccaaaca aaaaatatgt
    28021 aaatgataaa agtaagatgc ttgatttaga agggaatatt ataggaacca ctaaaagtga
    28081 aatcttaaaa aatataaata aaaaatgtca aggtgatttt ttatcttggg ataattggag
    28141 attatataat gtaaaatata cgaacataaa gtggactaat tggactttag tatcaaaatg
    28201 taatatattt aagaacttta aaaatctaat agttaatttt atgataaagt tgtattttta
    28261 tatagtaata tttatgatat tgtggaaatt aagtaatgct aaattaatag aacaacttaa
    28321 aaaattggct tattatgatt ctttaagtgg aataaaaaat aaagaaaagt ttagaaaaga
    28381 ttctatgtat attttaaaga attattatca agataatttt tacttagtac aattggatgt
    28441 caataaattt aaatatataa atgagatgtt tggatatgct gaagggaata agatattaat
    28501 tcatatatca caggttttaa ataataatac aaataaatat gaaatttgtg ctaggatgga
    28561 taatgaccat tttatattgt tgattgcttg taacactgaa gatgaacttt taaatagatt
    28621 atctaaaata aataaagaaa tttgtaactt aaatactact aactcatcta agtataaaat
    28681 tgttatgtca agtggaatat ataagattac taaaaaggat gatattaaaa agattgactt
    28741 gttaattgat agagcaaata ttgcagcaaa aagtaaaaag gaaaaatatg aacattcata
    28801 ttcatttttt aatgaagata caagaaatcg attgtacaag gaaaaacgat tggaagataa
    28861 tatgaataaa gctttagaaa aaggtgaatt tatagtatat tatcaaccta agtatagttt
    28921 agatgatgta aatgaaattg aaggagcaga agccttgata agatggaata gtccagaatt
    28981 tgggtttatt tcacctatag attttgtacc tttatttgaa aagaatggtt ttattgtaaa
    29041 tattgatatg ttcgtgtttg aagaagtatg caaaacatta aacaagtgga taaataaagg
    29101 gtatactcct gtaccaatct ctgtaaatat gtctagagtt catttatata gggataattt
    29161 tatagaaaat ataactgatt tgataagtaa atacaatata tctcctgagt ttattgaact
    29221 tgaactaaca gaaagtgttg tgtttgataa tttaaatata ttgattgata taatgaaaaa
    29281 gattaaagag ataggattcc ttatatcaat ggatgatttt ggttcgggat attcatcttt
    29341 aaatctgtta aaagaccttt cttttgatat tttaaaatta gatagaggat ttttaatcga
    29401 aacaacagat acaaaacgtg gaaaaattat tatttctaag attgtggaaa tggcaaaagc
    29461 gattgatatt aaagtaatct gtgagggcgt agaaacttat gaacaagtag agttcttgaa
    29521 agagataggt tgtgataagg ttcaaggata cttatttgct aaacctatgg tattagatga
    29581 atttgaaaaa cacttaaact ttaaatttga ttaatcatta ataattatta gactataata
    29641 taccctaaca acttacttac aaatatgtaa gttaattgtt agggtatttt tatggttaaa
    29701 gtagtatatt cacgatatta tggtattgta gtaaaaataa aaaattatgg agaatataaa
    29761 atgaacatat tgagtattag aaatatatca aaaacatatt gcggaaatat tccatttaaa
    29821 gcattagata aggttagtct aaatatagaa aagggagaat ttgtgtctgt tatgggacca
    29881 tctggaagtg gtaaatctac tcttttaaat ataatatcta cagttgatag acagagtgaa
    29941 ggtgaggttg tattagatgg atatgatgta agtaaattga aaggagaaaa acttgctgaa
    30001 tttagaagga aacaacttgg ttttgtattt caggatttta atttgattga cactttaact
    30061 gttggcgaaa atattatgtt gccacttaca ctagaggggg caagtatcaa agatatgaat
    30121 atacaaacta aaagtatatc taaattttta ggaatagata aaattttaga tagaaaaacc
    30181 tatgaaattt caggaggtca agctcaaagg tgtgctattg ctagagctat tataaataaa
    30241 cctgccatac ttcttgcaga tgagccaact ggaaatcttg attcaaaatc tactgatgat
    30301 gttttgaaat tatttactag gataaacaaa gaacaaaagg taacaacact tatggtaact
    30361 catgaagcat atagtgcaag ttattcagat agagtaattt ttataaaaga tggttgtata
    30421 tatacagaaa taaaaaaatc tgaaagcagt aattctttct attctgatat actagctgtt
    30481 ttgtctcaaa taggaggtgt aagatagtgg attttagaaa gtttgcttat aataatatag
    30541 ttaaaaattt cagggcatat ttagcatatt ttttaagttc tgttttttct ataatgatat
    30601 tttttgtttt tgcagtgtct atgttccatc caattataac aaaatctgga atgcagagtg
    30661 gttctactgc atttatggct cttatgactt cagaattaat aatattcggt tttagtctac
    30721 tttttatatt gtactcttta ggaaatttct tgaaatatag attgaaagat tttggagtat
    30781 tgatgataat tggaatgagt aacagacaac tgaaaaaatt gatattaatt gaaaatttga
    30841 taataggatt tttagcagtg atgttaggaa tattacttgg tttatcaatt tcaaaattgt
    30901 ttttattgta tttaagcaaa ctgttttata tgaatttaac tgaattttat tttccagtaa
    30961 aagcaatgct attgactatt gtatcattta tgattttatt tttgataaca ggaccaatga
    31021 gcttaaaatt attgagtaaa aagagcattt tagagctttt agtaggtaca aaaaagccta
    31081 aaaaagagat aaaatcttca aagctatttg gaataattgg tgtaatatgt cttatattag
    31141 gatatgctat gatactattt ggcaataagt taaaaataaa tggaggtatg tatacaacag
    31201 cactattaat tactttgggg atatttttat ttttttctca atttactatt ttgatattaa
    31261 agtatttaaa aaataaagaa aaattttata agaacaaagt aaatatgtta cttataagta
    31321 atttgatata taaattaaag gataatacac gattgttatt tttaataaca atacttttat
    31381 cggctacttt agtagcattt acaacaactt caacacttgt aagttctcaa ggagaagctt
    31441 ctaaaaataa ttttccaatg gtttactcat atttttcaga gcataataat gaaaaagaat
    31501 atgaagaact acaacaaatt agagaagcaa tcaaaggata tgattataag gagttaagtt
    31561 ttcctgtact taaatataat agggaagtct tactaagtgt aaagaattat aacaaattat
    31621 caaaggaatt gggattagat aaaattagtt caaagttaga aaaatatgaa ggggttataa
    31681 ttcctagtag caattcacaa gaacatataa attctttaaa acaattaaaa aattatagta
    31741 taaaaaattt aaatatagaa gtaaaaaaat caaaaaataa aataattttt cctacaggac
    31801 tgtttagtaa agttgtagtt ataagtgatg acttatacaa tgaagtaaaa ggtgaatttt
    31861 caaaaattaa ttttcatgga tttgattata aaaactggca aagcagtgca cagataactg
    31921 aaaatttaaa agaaaaacat tttaatttgg atagagaata taacaggtca tattttttaa
    31981 cattaccaga tatgtattta gtagagttac aacaaagtaa aattcttcaa tttatgggtg
    32041 tttttatagg agtgatatta tttatagcag tttttagttt tttatacttt agattatata
    32101 cagacgagat agaagataat gtaaagtaca aaaaactatc aaaaataggt ctatctttta
    32161 aaaatatgaa taagattgtt agtatagaga taggtacatt gttttttata ccgtatttta
    32221 tagcaattat aaatagcata atttctttac tgtttttaaa tcaagtatta aataatagtt
    32281 ttaagttaga aaatttgggt atattactac tgatatcatc tatatattca ctttattttt
    32341 acttattaaa aaaaatttat actagaaaag tttggttttt tgattaaagt ggtatataat
    32401 agatatataa attaatattt aaaaagtagt taatattagg ggagggcatt tgcacctccc
    32461 ttgtaatatg tggaaaattg aatattagaa gaaggaggag acttgatgga aaatcaacaa
    32521 ttattaggaa cagaacgaat tagtaaatta ctactaaaat attctatacc agctataata
    32581 ggtatgttgg taaatagttt atataatgta gtagatagaa tttttattgg aaatatacca
    32641 ggggttggac cacttgcaat aacaggtctt ggtgtaacaa tgccaataat gacaataata
    32701 cttgcatttg ggatgttgat aggtatagga actactacta ccatatcaat aaaacttggg
    32761 caaggtaaag tagaagaggc aagaaaactt ataggaaatg caatgacgtt atctgtaata
    32821 acaggaatta tcataatgat acttggaata ttatttgcaa ataaaatact tacattgttt
    32881 ggagcaagtg aaaatacttt aatatatgca aaatcatata tcaatattat attattaggt
    32941 acagtagtaa atcttctttc cttttcttta aaccactcaa taagagctga tggtagtcct
    33001 aaaatttcag caggaataat gatagtagga tgtttaacta atatagtttt agactggata
    33061 ttaatatttg gattcaattt gggaatacaa ggagctgcaa ttgctacagt aacttctcag
    33121 gctcttacag ctattttaac tatagggtat tatataagtg gaaaatcaaa cttacgattc
    33181 agtaaatcta atctaaaatt agataaaaag ctaataaaag cagtgtttgc aataggtatg
    33241 tcgccatttg ctatgcaact tgcagcaagt ttagttcaag taatatcaaa tattgcactt
    33301 aaaactcatg gtggagattt agctataggg gctatggcta ctatttcttc tattgcaatg
    33361 gtatttttga tgcctatctt tggaataaat caaggagcgc aacctattat aggtttcaat
    33421 tatggagctg aaaaatatga tagggttaaa aaagcatatt taggttcatt agtagtagct
    33481 actataattt tatgtatggg tatggtagta gttatgcttt tcccagaagc tattataggt
    33541 atatttaata aagaccctga acttatgaat atatctgtta atggattaag aatatactta
    33601 cttatgttgc caattgtagg cttatcagtt acaggaacaa actttataca atctatagga
    33661 aaagcaaaaa tggctatgtt attgagcctt ttaagacaag taattttatt gataccagca
    33721 gtattaattc ttcctacatt cttaggttta cagggtgtat ggacagctca accagtgtct
    33781 gattttatag ctacagtaat tacaggtata gttgtattta gagaattaaa aagatatact
    33841 cctaaaactg aaaaattaaa tgaaaatgaa agactaaacg agataactac tgagtaaaaa
    33901 tataaattca gataattgag caagaaattg caaaaaatac gattatatga taaaataaaa
    33961 ccgctgtcaa ggataagaga aattctaatt atggagtgat tttatggaat ttacaaagaa
    34021 aagcaaatta aaaaatatgt atgtaaacat gaatgtagct agaatgttgt ctaagataaa
    34081 tgagtataag ggtagacaac tgctttacaa aaaacaacca aaagaaattt tagaaaactt
    34141 agaaaaaaaa tctttagtag attgttctga atctacatac attggaaatc aaagagatag
    34201 tagtaatttt aatctagaaa agttgataag taatgaagtt acacctagaa gtagagagga
    34261 attgagtatt gttgaataca gagatgtggt aaaaactatt aacagtgctt atgagagtat
    34321 tcccatcagt tcacaaacga ttcttgagct tcatggatac ttatataaat tttcatctac
    34381 tagaggagga agttataagt cagataatga ttttattgaa cacaatttaa aacctagcga
    34441 atttataagt attgattcaa gtgtcaataa agttgaaaaa gctgtagaag aaatatgtga
    34501 agcttataat acattgatag aggaagatga aattgatata ttaatattaa tttctgcatt
    34561 tgtgttagat ttcatattga tacatccatt caaagaaggt aatataaaaa tggcgagagt
    34621 acttatccta ttgttactaa ataaaaatgg gtatgaagtt ggaagatata taagtttagg
    34681 aaagatattt gatgatagtt catatgaata ttatagtaat ttaaattatt tgaaagcctc
    34741 aataggtagt gaaaaagcag atatgaatgc atggattgag tattttttag agacgatatt
    34801 aactgcatat gagaagctgg atgatagttt aaatatatct gataagaaaa gacaaacaaa
    34861 aactagtaga atagagaaaa taataaattc tacccttgga tattttacaa aagaagatat
    34921 aagagattta tgtcctgaca ttccagaacc tactataaat agagttttta ataatctaag
    34981 aaaacaagat aagatagaag ttgttgcaag aggaagaagt gcaaaatgga agaaaaaata
    35041 ttaaaaaatg tttagataac taaaatggta tagtgtttta cactatacca tttttaatgt
    35101 cattagcatg agcaataatt taacaactta agcaatatca attttattta ttgattattt
    35161 ttgaactgaa aaatggcgtt atatggtata cctcagtggt aattaatttt ggatgagaag
    35221 atataatatt acagaatata ttaggagtat atgtagaaaa aatataataa aagtgtgagg
    35281 cttagtaatt aacaaattat gtggcttcac acttttttat aaaataaaga tacttagcag
    35341 aagaatattt tagttgatat gtttataaga tggtacttgt ttgggtaaaa gtataaatag
    35401 taaaaaagta gaagtaatgt gtataaaaac gtaagttaca ttctgtaata atagttattt
    35461 tataattcaa caaaagaata gaatgggtat atttgtgaaa taataatata aaataatctt
    35521 aggagtgagc ttatgaagaa aaaattatta tatataaatg taaattcaaa acctgaagac
    35581 ttatcttcaa gtaaaactgt agcaagaaaa tttataaata aatttatgga aaaaaataaa
    35641 gattttgagg ttgaagagat agacctttat aaagaacata taccaagact tgagtatcag
    35701 tactttgaaa aaagaaatag catagttagt gaagaaaatg ctaaaaattt agacgataag
    35761 gatagaaaag aacttacaaa gattagaaat ctttgtgacc aatttgtgag tgcaagtgta
    35821 tatgtaatag cagcacctat gtggagttta tcttttccag caccacttaa agagtatata
    35881 gattgtataa ttcaagatgg aaaaactata tcttttgaag gcaatgataa accacaagga
    35941 attttaaatg atattgatag aagtatggta tatatacaat cttctggtgg acacatacca
    36001 tgggtgttga agccagttat gaataaaggg ttaaattatg tggaaagtat tatgaaattt
    36061 ataggaatta aaaaatttga tgaattgtta gtagatggaa ctggaactag tgaagaagaa
    36121 agacaagctg ctatagaaaa agcagctgaa aaaatagatg gtataataga tgggatgaaa
    36181 ttttaattat aatattttat taaaaatata aaggaaaaac ctcttttaat gtagaaactt
    36241 atattgaagg aggcgagaat catgcgagaa gaaaaaagta atgaaaagta tgattgttat
    36301 tggtgtaatc aagagaataa tttctgtgta gaaataaaag ataatatagt catgatagat
    36361 gatggcactg gtacgttaaa acaagccgtt ttcatagggt ataaacaaat ccaaattaat
    36421 ttaaactgtt cacattgtca aaacttgaat agaataaaat taaatttgta gaattttaaa
    36481 atggaaatta ttgaaggaat taaaaataga aagtgttaat atttagactg aaaattatat
    36541 tttatacaat aaaagtttat ttaaataaca ttgtatttta atatttattt tcagtcttac
    36601 tttattaaaa ttattttttg gttatgtctg gaaaatatct gtctactaat ttttctgaaa
    36661 tattttttaa ttggtcagcc attgaagtca gacttaataa ggctatcaat aaatcatctt
    36721 tattttctgg atttatacta atattttcaa aattagactc tataaagtcc ggtatattct
    36781 ttctttgatt ttcttttatt aacatgaatt tttcgtaaca tgaaactaaa tctttttcac
    36841 tgaaattatc cttttcatac acaccatgta agattctttt aatttcttgt attgtaagtg
    36901 tatttttcat agaataaata attatcatct gtaaaatttg ttctttagta tacttttttc
    36961 ccttaactgg tttgattaat ccctctttgc tgtaattatt tatcatggtt ttagtaagaa
    37021 ttttatcact ttcaaatctt ttattggcag aaagcttatt gtcaattaga gaaataattt
    37081 gatccatata taagtcaata gatggaatat cgtttgaagt tatatctgca ttatttagtg
    37141 tttctagtat tatagtatta agttcattat tcatcatatg ttacctccat aatatatata
    37201 atacactaaa ttagaaagta aaacaatatt aaggcttgta aaaaaaacat aaagtggtat
    37261 aattaaatat ataatatagt aatggaaact atatttgtga atctgatttt taaaatacat
    37321 aattgtagga ggacgaaatg tatcaatttt ttttaaaggg tagagaccca ataagcagtt
    37381 taactcattt tataggtgct tgtttatctc ttttagcaac tataatttta gtttttcaat
    37441 ctgttacatt acaggaaact tctctcttga tgatagtatc tgtatcggta tttggatttt
    37501 cactaattgc tttatatagc gcaagttcgt attatcattt tttaaagggg acaccagaac
    37561 aagaattgtt ttttagaaaa gtagaccatg caatgattta cgtattgatt gctggctctt
    37621 atacaccaat atgtctgaat tttatggaaa aaaaagaagg cataatattt gttacagcaa
    37681 tatggatagt tgcatttatt ggtataataa taaaaatatt ttggatggat gcacctagat
    37741 ggttatctac cagtatatat ttacttatgg ggtgggcaat tgtttttgat ataaatgcat
    37801 tcaattcaat tcctaaggat tgccttagac tattaattat ggaaggtgta tcttactcta
    37861 taggtgcaat tatttatata attaaaaagc ctaatataag tcctgagttt ggatttcatg
    37921 aaatatttca tatttttatt atgataggtt ctttatttca ctttttagca gttttattgt
    37981 atgttttata gttttaaaag atttgattaa ttgatactaa aattctacaa tgaataaaat
    38041 ggaactgcct tatatagatt atatcttcga caataagaca gttccttatt ataaatatta
    38101 aattctactt ataataaaat tttgataaaa atagtccatt caaaatgcct attccataat
    38161 tcctttaatg tacttttcat ttacgacata tagtttgaat ttatgtacta taattatatc
    38221 ctctaaattc tctgaaacac taacaattct gacacatttg tttctaattt ctacaggtaa
    38281 atctaaatca tttgttaaat tcacatagtc atcatattta tacataaaat catcccttta
    38341 cattaagata tacttattat actatatttt aataagtttt tttaaattat tacaattttt
    38401 cccgaaattt cattttttat acataaaatt tccaaattag gtaaagatta aattttttat
    38461 tgtataaaca aaagttacat ataaaatatt ttattcaatt ttttaaataa ctattgccaa
    38521 tttttgaaaa atatattatt attaatagta acaaaaaact gtaaatttag aatattaata
    38581 aaaattattt gggaggaaat tatcatggtg agattaaata tagtaaaatt aaatacaaaa
    38641 ttaaatcatc accatcatca tagattaaag ggattgtaat atgaaattaa ttcatagaca
    38701 caagccctgt ttttagtgta attggtcttg tatctatgat gggaagtaat tttaagagaa
    38761 aattatagcc atataggtat aaagcctatg tggctttttt attgttttaa atttgcaaat
    38821 ataagagtaa atttaatgaa gagggtgaga atgtgaaatt tttgaggatt gaagatgagg
    38881 ttatttattc taaaaaaaga tataaattaa aaagggaaat agataaactt tttttggatg
    38941 aagaatattt tcaaatagaa ccacaattat ttgaagaata tgatgaattt acaactataa
    39001 ataataaaat tccagaagaa tctatggtaa aggttgtaaa tggaaaagta atggtactta
    39061 gagctgatat aactacaagt ataataaaaa gccttgtacc aagatgggaa gatggtttaa
    39121 agcttaagct tttttataat tcatcaatat ataaaaataa aaatacagtt ggaataaaag
    39181 agataagaca aataggttgt gagtatctag gagaagcctc tgtagaagca gatagagaag
    39241 tagtgaaatt agctcttaag atacttgaaa aatacaataa taattttatt ctagaggttg
    39301 ggagtagcaa gtatattcat ggtttgttag aagaattaaa tttaaataag aattgtgaaa
    39361 atcaaattaa aaatttacta tatacgaaga acactcatga actaaaagta tatattgaag
    39421 aattaaaaat taagcgtgaa gtaaaagagt tattatcaaa tattttaagt ttacaaggga
    39481 atttatataa tgttattgaa aaatcaaaca agttctattg taataataag atgaagcaag
    39541 ctcttgaaga attaaagcaa gtaaataatt taattgagga atgtaatttt ttagataagg
    39601 ctagatttga tttatcaatg attactatgc ttgactatta tgaaggaatt atgtttagag
    39661 gatactaccc aaactcatat aaagaaattc ttagtggcgg aagatatgat tctttaacta
    39721 aagagtatgg aaaagaaatt ccagccattg gatttacact aagtgttgat gagttaatga
    39781 agtatgtata taagtaaagt ttagaaggag ggattgcttt ggattatttg actatagcac
    39841 tagcaaaagg acgaattgaa ggggaatcat ttaaaaaatt taaaaaaatg ggtctaggag
    39901 acagtataga cacagatacg agaaaactaa tttttaaaga tgaagaaaat aaaattatat
    39961 atatacatgt aaagccatca gatgtagtta catatgttga aaaaggagtc gctgacctcg
    40021 gtatagcagg taaagatact attttagaaa atgagacaga tgtatatgaa atatatgatt
    40081 taggttttgg aaaatgtaaa tttgctgtag caggattaaa gggagatagc atatatagag
    40141 aagatgaata tttaaaagtg gcaacaaagt atcccaatat agcaaaaaag tattttaaag
    40201 agaaaggtca aaaaattgaa ataataaaat taaatggttc tgtagaacta gctccaatag
    40261 tagggttgtc agatgtaatt gtagatatag ttgagactgg caatacactt aaagcaaatg
    40321 gtcttgaaat attagaagac atatgtaata taagtgctag aattatatca aatagagcta
    40381 gctatagatt taaatatgaa caaattcaga atataattcg tttatttgaa gaacttgata
    40441 attaaaatat aattgagctt tataaggagg gatttaagtt gagagaaaaa gaaagcataa
    40501 gagagttaag gggatatgaa ccaaatcacg taaactgtaa agtaaaactt gatgcaaatg
    40561 aaggaagtaa gaggttgttt aaatacctta ttaaagaaat ttcagatagt gatatagatt
    40621 taaatttata tcctgaagat tcttatagtg atttgaaaga atctataata gattacataa
    40681 atatttctgg agtaaataag aaaaatcttc tagtaggaaa cggttcaagt gaaataattg
    40741 atttaataat acatactttt gtagataaag atgaagtaat attgagtttc tctccaagtt
    40801 ttagtatgta ttctatatat agtcagataa atgggtctaa atttataggt gttgaaagtg
    40861 atgaaaattt agttataaat atagataatg tgattgaaaa agtaaaggag aataatccta
    40921 aaatagtaat tgtatgtaat cctaataatc caactggaac aatattaaag agggaagaga
    40981 taataaagct tttagattca actaatagtc tagtggtttt agatgaagca tatatggatt
    41041 ttggagaaga aagcatgtta agtgatgttt ttaagtatga taatctaata gttcttagaa
    41101 ctttatcaaa ggcatttgga ttggctggaa taagaacagg atatatgtta tctaatagta
    41161 gtttaataaa ttctgttgag aaagtaagac ctccatataa tttaaattca ttatcagact
    41221 ttattgctac aagagcactt agaaataaag atgtggtaaa ggcttatata aaggaagtaa
    41281 aagaggaaag agaagtttta tatgaagaaa tgattggtat gggaattaaa gcatacaaat
    41341 cacaagcaaa ttttatatta ttttattctg aaatcgaaaa tttatcacaa aaacttattg
    41401 atagaggtgt tttaataaga aaatttggtg gaaagttaga aaattattac agagttacaa
    41461 ttggtgataa agaagaaaat tctatgtttg ttggagctat aagagatatt ttgaaaaagg
    41521 agaaataggt atgagaattt ggaaggtaga aagaaatact cttgaaacac agattttagt
    41581 agaattaaat attgatggtt ctggtaaagc tgaaatagat actggaatag gatttttaga
    41641 ccatatgctt accttaatgt catttcatgg caagttcgat ttaaaagtga tttgtaaggg
    41701 tgatacatat gtagatgacc accatagtgt agaagatata ggtatagcta taggtgaagc
    41761 atttaaaaat gcactaggtg acaaaaaagg aatacgaaga tattctaata tctatatacc
    41821 tatggatgaa tctctatcaa tggtagcaat agatataagc aatagacctt atcttgtatt
    41881 taatgctaaa tttgataccc aaatgattgg tagtatgagt actcaatgct ttaaagaatt
    41941 ttttagagct tttgtaaatg aatcgagagt aacattacat atcaatctct tatatgggga
    42001 aaatgaccat cataagatag aaagtatttt taaagcattt gcaagagctt taaaagaggg
    42061 tagtgaaata gtttctaatg agatagcatc ttcaaagggg gttttgtaga atgaatataa
    42121 tagtagacta tggtcttgga aatatagatt ctgtgtctag gggttttaga aaagctggaa
    42181 tagaaactaa aatttctagt gatatagatg aaataaaaca ggcagattca cttatacttc
    42241 caggtgttgg agcttttaga gattctataa gtgccttaga taaactagga ttgataccaa
    42301 taataaagga gcatgtttct aagggtaaat ttatgatagg tatatgtcta ggaatgcaat
    42361 tactttatga aaaaagttat gagtatggag aatatgaagg attaggactt ataaaaggaa
    42421 gtattgataa actggatata agtttaaaag tgccacatat gggatggaat aatctaaaat
    42481 ttaataaggc aaatgatgat atacttaaat atataaatga agatgattat gtatacttcg
    42541 ttcactcata ttatgcaaat tcatcaaatg aagaactaat tgcattttca gaatatgaaa
    42601 agaaaatccc tgctattgtt agaaaaggca atgtatatgg tatacaattt catcctgaaa
    42661 aaagtggaga agtagggtta aatatattaa gagcatatgg ggagatgata aaatgataat
    42721 atttccagca atagatataa aagataataa gtgtgtaaga ctcacacaag gagaatttga
    42781 caaagtaaat gtatattatg ataatccatt agaagtagca tataaatgga aaaatgaagg
    42841 agcagaatat atacatatag tagatttaaa tggtgctaga agtgaatttg gtgtaaatac
    42901 aaaaataatt gaggacatag caaacaatat agatatacct attcaagttg gcggtggagt
    42961 tagggataag gaaaaagtca aaagtttaat aaatgctggt gttactagag taatattagg
    43021 tagtatagcg attgaaaatt tgaatttagt tgaagaattg gtcaatgaat ataaagagaa
    43081 aattgtagtt tctatagatg ctaaagatgg aaaagtagca gttaggggat gggaagttgt
    43141 aagcaatgta gactcattaa cattatgtaa acaacttgaa aagataggtg tacaaactat
    43201 tgtttataca gatatatcaa aagatgggat gttgcaaggt ccaaattttg acatatatga
    43261 aagaatagca aaggaaacat cactaaatgt tattgcttct ggaggcgtaa cttctataga
    43321 agacgtaaaa agactaaaag ctatgaactt atatggtgca ataataggta aagcgcttta
    43381 tgataaaaag atagacttta aggaggcaca acaattatgc ttactaggag aataatacct
    43441 tgtctagatg tgagaaatgg aagagttgta aaggggaaaa aatttaaaga tatagtagat
    43501 gttgatagtc cagaagtact tggaaaattt tatagtgatt gtggtgcaga tgaacttgta
    43561 ttctatgata taactgcatc aaatgaagag agaaagacat ctttagaatt tgtgacaaaa
    43621 gttgcagaaa atataaatat accattttgt gttggtggag gagtaaataa attagaagat
    43681 ttcactgata ttttaagaaa aggtgctgat aaagtttcta taaattcatc agcagtcaaa
    43741 aatcctgaat taataaggga agcttcatta aaatttggag cacaatgtgt tgttttatcg
    43801 atagatgcaa agaaaaatga agagggttca tggagcgtat acgtaaaagg tggaagagaa
    43861 aaaactaatc tggatgcaat tgaatgggct gttaaaggtg tagaacttgg agcaggagaa
    43921 atcgttgtaa atagtatgga tgaagatgga atgaaaaatg gatatgatat agaattatta
    43981 tcaaagataa cttctttagt aaatgtacct gttatagcat ctggaggcgc tggaaaaaag
    44041 gaagattttt atgaggcggt taataaatcc aatgtagatg gaattttagc agcatctgtt
    44101 tttcattttg gcgagataaa aataaacgat ttaaagaaat atttaaaaga catgggtgta
    44161 gaagtaagat tataagttaa atactaaaag gagattagaa atatggataa taagtgtaac
    44221 aatgtataca gtgatgaagt agagaaattt ataagaagta taaagtttga tgataaaggg
    44281 ttagtccctg ttgttgttca agaggtagtt agcaaagatg tactgatgtt agcatatatg
    44341 aataaagatg tgataaagaa aaccttaaaa gataaagtag cttgctattt tagtagaagt
    44401 agacaagaac tttgggttaa aggtgagaca tctggtaata ctcaaaaagt agttaagatg
    44461 tcttatgatt gtgatgtaga tactatacta ctttttgttg aacaaactgg tgtagcttgt
    44521 catacaggca attattcttg tttttacaga gacttgtttg atgatagttc aaaaatggag
    44581 cttgaagtac aaacaaatat attgaaagaa ttatatgatt taatcaatga aagaaaaaat
    44641 aatccagttg aaggttctta tactaattat ctttttgaaa aaggaataga taagattctt
    44701 aaaaaagtag gtgaagagag tagtgaggta attatagcat caaaaaatac tgataaaagt
    44761 gaacttattt acgaaattag cgatttagta tatcatacat tggttttaat gatagaaaaa
    44821 ggtgttgaaa ttgatgaaat aaaaaaagaa ttacttaaaa gaagaaaata gaataagata
    44881 gattaatggg taataagaaa atataccact tttaataaaa agtggtatat tttttatttt
    44941 atattttgat ttatagaata atatttaaat tacttgataa aattaactat atatataatt
    45001 tattgataaa ataattctag tacattattt attttagaaa aaagataatt atctaattat
    45061 atttaaggat aataatatga aaataatttt tatattaaaa aatatgaatg aaaatagtag
    45121 gttttataaa aaaacatatt tagattttaa catctcaaag gagttttcaa agctaatatt
    45181 tcatttttaa ataaaaaaat aaaatattag ttttgatttc tggtttaaat tgggttataa
    45241 taaattgtca aattatttat taatataaaa tagactataa aacaagcaaa aatcatacta
    45301 ttatataaaa gaccttaaag atagaaaact acagaaagaa gccaaaatgt ttttgaaaaa
    45361 aatgaaattt tattgcaaaa aaatcaaaat gaatttaact taatagttat aatctatttt
    45421 tagtgttaca aatatacaat gtatattata caatataaaa aatacattta cttagaagta
    45481 ttattatatt tggatttttg gtttttataa tttttttata tttaaacata actaaaaata
    45541 aaagattact aaagctaagt agagtttatt ttattttaat aaataataaa gctatttttc
    45601 tatatattgg cataatatat gcttatttaa ctatgaaatt aaaattaaat aggattaatg
    45661 aggaggaaaa aaatgggaag taatgaactg aaaaagactc ttggcgtatc agcagcatta
    45721 tcaactgttg ttggttccgt tataggagcg ggcgtatttt ttaaaccaca agcagtctat
    45781 acattaactg gaggagctcc aggtcttggt atattggcat ggttaatagc tggaatcata
    45841 actataacag ctggtcttac agctgcagag gtatcagttg cgataccaaa aactggtgga
    45901 atgatggtat atataaaaga aatttatgga gaaaaattag gttttttaac tggatggatg
    45961 caaattgtat tattttatcc agggatgatg gcagcattag gtgttatatt tggagaacaa
    46021 gcatcggcac taattggtag tccatcactt ttacttccaa tagcaattgg aattataata
    46081 atagttgcag ggcttaatat gttaggttca aaaactggag gagtcataca aactgtttct
    46141 acaatttgta agttaatacc acttatttta ataatggttg ttggatttat taaaggtggt
    46201 ggaaataatc caatacttac acctatggtt ggtgaaggat taagcctggg tagtgtacta
    46261 ggtcaagttt taattgctat attatttgct tttgatggat ggatgaatgt tggtacttta
    46321 gctggagaaa tgaagaatcc tggaaaagac cttccaaaag ctataatagg tggattgtca
    46381 gttgttatgg ctgtttattt tataataaat ttagcgtatc tttgggtatt accagctaat
    46441 gaattagcaa attatgcttc tcctgcatca gcagttgctg aagttatatt tggttcaatg
    46501 ggtggaaaaa taatatctgt aggtatatta atttcagtgt ttggagcatt aaatggattt
    46561 ttactaactg gttcaagggt ggcatatact ttagctacag ataagacttt accaaagtat
    46621 ggtatatttt ctaaattaaa ttctgctcaa gttccagcaa atgcaatagc attagtatct
    46681 gttattgcat ctatatatgc tttgtcaggt caatttaatt tattaactga cttagctgta
    46741 tttgctactt ggatattcta tgtattaact tttatagggg ttatgaaact tagaaagact
    46801 catccaaata taccaagaga gtataaagtt ccattatatc ctattgttcc tataatagct
    46861 atagcaagtg gtatatttgt tgtagttaat caattgtgtt ttgcaggtat gaaaactact
    46921 atgatatcta taggaggtct tgtaataaca gctataggct taccagtgta tgcttatatg
    46981 actagaggag taaaaagata aaccaagtag tttacaaatg cttcataatt aaaataaaag
    47041 agactgtctc aaatagagat aagtctcttt tttattgtta aaatggtaaa gatatgttta
    47101 attttacaaa aaaagagcgt tccatgaact aaaaagttta ttttaaggta ttcattttat
    47161 ttttagtttt ttgattataa aattaaacat ctaaaaaatt atttatttta aaatacttta
    47221 ttttaaagtg ttttttatca aactaagtgg tatctgtaca acaaatatta tacatagctt
    47281 ttatgaaaat taaatctttc tgttatatat aatagaatat tgtattatat agaaaatatt
    47341 acctgattaa tatagtgatt tataaatgag attagaaaat tttatttgat
//
LOCUS       DAYAOY010000022        46315 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYAOY010000022 DAYAOY010000000
VERSION     DAYAOY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.022"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            465..587
                     /locus_tag="ACGI6B_001300"
     CDS             465..587
                     /locus_tag="ACGI6B_001300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750754.1"
                     /translation="MMDLEKEKHEIIEIIKKLNIDKDKRNLLMIKNYILGLLKK"
     gene            complement(602..988)
                     /locus_tag="ACGI6B_001301"
     CDS             complement(602..988)
                     /locus_tag="ACGI6B_001301"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750755.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            1136..1279
                     /locus_tag="ACGI6B_001302"
     CDS             1136..1279
                     /locus_tag="ACGI6B_001302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750756.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            1284..1508
                     /locus_tag="ACGI6B_001303"
     CDS             1284..1508
                     /locus_tag="ACGI6B_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750757.1"
                     /translation="MKRLKISDFNKNQIGSAVQLQDIRFDTKADCIITKVEKNEILVM
                     YYEKETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            1527..2267
                     /locus_tag="ACGI6B_001304"
     CDS             1527..2267
                     /locus_tag="ACGI6B_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750758.1"
                     /translation="MITLDLLKDARKKEWFWLENDLIDREDLSIYEKMIYIVLARYSN
                     EESSCFPSYKTISEKAGCSIRQVSNILNELENKELIIRENRNHEGKKEKNSNLYFLIS
                     SKAKVQNEVPNVRHEVPNPMAPGALHVRHEVPSKKTYNKKTYIKSNTTTQKESKTDYL
                     DLSFLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALL
                     TWGNGDASKGIVPEVTKTKNPLSGFKEL"
     gene            2295..2480
                     /locus_tag="ACGI6B_001305"
     CDS             2295..2480
                     /locus_tag="ACGI6B_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750759.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            2506..2724
                     /locus_tag="ACGI6B_001306"
     CDS             2506..2724
                     /locus_tag="ACGI6B_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750760.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(2743..2949)
                     /locus_tag="ACGI6B_001307"
     CDS             complement(2743..2949)
                     /locus_tag="ACGI6B_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750761.1"
                     /translation="MCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNKNY
                     ICKIQIMTANSNCPLFKKARQLQY"
     gene            3118..3378
                     /locus_tag="ACGI6B_001308"
     CDS             3118..3378
                     /locus_tag="ACGI6B_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750762.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(3371..3559)
                     /locus_tag="ACGI6B_001309"
     CDS             complement(3371..3559)
                     /locus_tag="ACGI6B_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750763.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            3697..4014
                     /locus_tag="ACGI6B_001310"
     CDS             3697..4014
                     /locus_tag="ACGI6B_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750764.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            4036..5367
                     /locus_tag="ACGI6B_001311"
     CDS             4036..5367
                     /locus_tag="ACGI6B_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFG9750765.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            5742..6128
                     /locus_tag="ACGI6B_001312"
     CDS             5742..6128
                     /locus_tag="ACGI6B_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750766.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            6181..6885
                     /locus_tag="ACGI6B_001313"
     CDS             6181..6885
                     /locus_tag="ACGI6B_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750767.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            6942..7124
                     /locus_tag="ACGI6B_001314"
     CDS             6942..7124
                     /locus_tag="ACGI6B_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750768.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            7121..7498
                     /locus_tag="ACGI6B_001315"
     CDS             7121..7498
                     /locus_tag="ACGI6B_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9750769.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            7515..8063
                     /locus_tag="ACGI6B_001316"
     CDS             7515..8063
                     /locus_tag="ACGI6B_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9750770.1"
                     /translation="MRSLEERNKLVEENINFVWFIIRRYFKVFISKYPYLQDDTFSEG
                     CIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRADIDSFEKCIYKNT
                     HGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREKGYTQKEIGKILR
                     TNQMTICRKLGKLKSEINILGK"
     gene            8093..8251
                     /locus_tag="ACGI6B_001317"
     CDS             8093..8251
                     /locus_tag="ACGI6B_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750771.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            8254..8580
                     /locus_tag="ACGI6B_001318"
     CDS             8254..8580
                     /locus_tag="ACGI6B_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750772.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            8580..9116
                     /locus_tag="ACGI6B_001319"
     CDS             8580..9116
                     /locus_tag="ACGI6B_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFG9750773.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            9128..9934
                     /locus_tag="ACGI6B_001320"
     CDS             9128..9934
                     /locus_tag="ACGI6B_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750774.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            9959..10219
                     /locus_tag="ACGI6B_001321"
     CDS             9959..10219
                     /locus_tag="ACGI6B_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750775.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            10243..10728
                     /locus_tag="ACGI6B_001322"
     CDS             10243..10728
                     /locus_tag="ACGI6B_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750776.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            10748..11005
                     /locus_tag="ACGI6B_001323"
     CDS             10748..11005
                     /locus_tag="ACGI6B_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750777.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            11114..11557
                     /locus_tag="ACGI6B_001324"
     CDS             11114..11557
                     /locus_tag="ACGI6B_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFG9750778.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            11700..12245
                     /locus_tag="ACGI6B_001325"
     CDS             11700..12245
                     /locus_tag="ACGI6B_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750779.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            12249..12893
                     /locus_tag="ACGI6B_001326"
     CDS             12249..12893
                     /locus_tag="ACGI6B_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9750780.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            13114..13566
                     /locus_tag="ACGI6B_001327"
     CDS             13114..13566
                     /locus_tag="ACGI6B_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750781.1"
                     /translation="MNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIGVN
                     RKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVMLA
                     ANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            14121..15860
                     /locus_tag="ACGI6B_001328"
     CDS             14121..15860
                     /locus_tag="ACGI6B_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750782.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            15876..16064
                     /locus_tag="ACGI6B_001329"
     CDS             15876..16064
                     /locus_tag="ACGI6B_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750783.1"
                     /translation="MANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRARSKSY
                     ISKQDIPIKVLKFIESTG"
     gene            16077..17354
                     /locus_tag="ACGI6B_001330"
     CDS             16077..17354
                     /locus_tag="ACGI6B_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_process: GO:0099001 - symbiont genome ejection through
                     host cell envelope, long flexible tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9750784.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            17332..18093
                     /locus_tag="ACGI6B_001331"
     CDS             17332..18093
                     /locus_tag="ACGI6B_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9750785.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            18152..18478
                     /locus_tag="ACGI6B_001332"
     CDS             18152..18478
                     /locus_tag="ACGI6B_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750786.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            18504..19106
                     /locus_tag="ACGI6B_001333"
     CDS             18504..19106
                     /locus_tag="ACGI6B_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFG9750787.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            19135..20058
                     /locus_tag="ACGI6B_001334"
     CDS             19135..20058
                     /locus_tag="ACGI6B_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750788.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            20072..20344
                     /locus_tag="ACGI6B_001335"
     CDS             20072..20344
                     /locus_tag="ACGI6B_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFG9750789.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            20337..20708
                     /locus_tag="ACGI6B_001336"
     CDS             20337..20708
                     /locus_tag="ACGI6B_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9750790.1"
                     /translation="MLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELSTAL
                     EGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENSISS
                     SSFLTDTDKKFLSPFCIVSMY"
     gene            20720..21067
                     /locus_tag="ACGI6B_001337"
     CDS             20720..21067
                     /locus_tag="ACGI6B_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750791.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            21067..21423
                     /locus_tag="ACGI6B_001338"
     CDS             21067..21423
                     /locus_tag="ACGI6B_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9750792.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            21432..21881
                     /locus_tag="ACGI6B_001339"
     CDS             21432..21881
                     /locus_tag="ACGI6B_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9750793.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            21882..22937
                     /locus_tag="ACGI6B_001340"
     CDS             21882..22937
                     /locus_tag="ACGI6B_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9750794.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            22953..23393
                     /locus_tag="ACGI6B_001341"
     CDS             22953..23393
                     /locus_tag="ACGI6B_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9750795.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            23408..23824
                     /locus_tag="ACGI6B_001342"
     CDS             23408..23824
                     /locus_tag="ACGI6B_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9750796.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            23851..24000
                     /locus_tag="ACGI6B_001343"
     CDS             23851..24000
                     /locus_tag="ACGI6B_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750797.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            24004..27393
                     /locus_tag="ACGI6B_001344"
     CDS             24004..27393
                     /locus_tag="ACGI6B_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9750798.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            27440..27916
                     /locus_tag="ACGI6B_001345"
     CDS             27440..27916
                     /locus_tag="ACGI6B_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFG9750799.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            27974..28609
                     /locus_tag="ACGI6B_001346"
     CDS             27974..28609
                     /locus_tag="ACGI6B_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9750800.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            28850..30367
                     /locus_tag="ACGI6B_001347"
     CDS             28850..30367
                     /locus_tag="ACGI6B_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9750801.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            30381..30713
                     /locus_tag="ACGI6B_001348"
     CDS             30381..30713
                     /locus_tag="ACGI6B_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HFG9750802.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            30706..31140
                     /locus_tag="ACGI6B_001349"
     CDS             30706..31140
                     /locus_tag="ACGI6B_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9750803.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            31133..32185
                     /locus_tag="ACGI6B_001350"
     CDS             31133..32185
                     /locus_tag="ACGI6B_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9750804.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            32187..32795
                     /locus_tag="ACGI6B_001351"
     CDS             32187..32795
                     /locus_tag="ACGI6B_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9750805.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            32812..33594
                     /locus_tag="ACGI6B_001352"
     CDS             32812..33594
                     /locus_tag="ACGI6B_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9750806.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            33611..35056
                     /locus_tag="ACGI6B_001353"
     CDS             33611..35056
                     /locus_tag="ACGI6B_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750807.1"
                     /translation="MKIWKYNRTVKKRRGVYTELDILAQRYEFEADGKLYTAYFHLYT
                     NKNIDIFRDKDLYKESYMMSYRVGNVTTDIDDVVQQTTTVRFIPDPVIDIVIDNISRG
                     CEINYRIIDNEPSVKFIVTEKLNGTIIETKTNSSDNNYKLKLTDEQIVPLAFNSQNSI
                     VIEISTEDGGLVTSKTVTFTRTNNKPTILVNSYSSNSAKFTASDLDNNLSKIEWYLDD
                     VLKETITTDLTAEKTINYEFTDNAIHTLKIVATDAENATVEKVLSISKEIMPLQSDAS
                     LSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQ
                     DLQNRITELNNQLSRRKKWATGRYTFTREEDTINFRMVDGDSLSAVKKLIIPINLDFV
                     PSVIIIDKMFLGSYVPAQDVQFNCVSNLADFTLGRRFYNKQWICTSSIKLDSVTQNSF
                     TLSLQKVVPFDKVSSTIFSIINTGFAWRAYE"
     gene            35080..35427
                     /locus_tag="ACGI6B_001354"
     CDS             35080..35427
                     /locus_tag="ACGI6B_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750808.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            35439..35609
                     /locus_tag="ACGI6B_001355"
     CDS             35439..35609
                     /locus_tag="ACGI6B_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750809.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            35639..36208
                     /locus_tag="ACGI6B_001356"
     CDS             35639..36208
                     /locus_tag="ACGI6B_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750810.1"
                     /translation="MTFKELVSKVRNLVLEAKNVTIEDTENKFTSENVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATSEQTFQEYANYIAGFKNTISQLNSKYKYTSGTFT
                     YYTGAYPNGLNIGFRPDIISYTAINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNR
                     YGITFTDNGWIPRNPVNNGVYSYIAAKCR"
     gene            36267..36695
                     /locus_tag="ACGI6B_001357"
     CDS             36267..36695
                     /locus_tag="ACGI6B_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9750811.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            36699..37529
                     /locus_tag="ACGI6B_001358"
     CDS             36699..37529
                     /locus_tag="ACGI6B_001358"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9750812.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            37601..38707
                     /locus_tag="ACGI6B_001359"
     CDS             37601..38707
                     /locus_tag="ACGI6B_001359"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFG9750813.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            38914..39180
                     /locus_tag="ACGI6B_001360"
     CDS             38914..39180
                     /locus_tag="ACGI6B_001360"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9750814.1"
                     /translation="MSKKEIIRICNEKHNWRKSATKTLLKRLVKKRVLLKRIKIFHIY
                     YIPIVTEKEYFKYKINDLEPDYLDEYFIRLMSTVHKFKNLDEED"
     gene            complement(39191..39706)
                     /locus_tag="ACGI6B_001361"
     CDS             complement(39191..39706)
                     /locus_tag="ACGI6B_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750815.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(39789..40694)
                     /locus_tag="ACGI6B_001362"
     CDS             complement(39789..40694)
                     /locus_tag="ACGI6B_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFG9750816.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(40714..40917)
                     /locus_tag="ACGI6B_001363"
     CDS             complement(40714..40917)
                     /locus_tag="ACGI6B_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750817.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(41166..41624)
                     /locus_tag="ACGI6B_001364"
     CDS             complement(41166..41624)
                     /locus_tag="ACGI6B_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750818.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(41659..41961)
                     /locus_tag="ACGI6B_001365"
     CDS             complement(41659..41961)
                     /locus_tag="ACGI6B_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750819.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(42349..42501)
                     /locus_tag="ACGI6B_001366"
     CDS             complement(42349..42501)
                     /locus_tag="ACGI6B_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750820.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(42621..43355)
                     /locus_tag="ACGI6B_001367"
     CDS             complement(42621..43355)
                     /locus_tag="ACGI6B_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9750821.1"
                     /translation="MYYVYKYINLDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RFEYIELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(43375..44562)
                     /locus_tag="ACGI6B_001368"
     CDS             complement(43375..44562)
                     /locus_tag="ACGI6B_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFG9750822.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            44982..45671
                     /locus_tag="ACGI6B_001369"
     CDS             44982..45671
                     /locus_tag="ACGI6B_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9750823.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(45820..46203)
                     /locus_tag="ACGI6B_001370"
     CDS             complement(45820..46203)
                     /locus_tag="ACGI6B_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9750824.1"
                     /translation="MKIKKIPESELLVMMFIWDSEDKEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
BASE COUNT        18599 a         5392 c         7720 g        14604 t
ORIGIN      
        1 aaaaaaaaat taagcaggag taaaaaaaat aaaatattat caaaatttat caatataaaa
       61 agaaatataa gtataaattt ttctctttgt atattgattg tatacaattc tatttttgta
      121 aaattaaatt ttaaaaaata atcaaaaatg gtaaattttg acatttgctt aatgtcaaat
      181 taggtgttac aattgtagca taaagaagat atatatcgaa ataatacata taaaactacg
      241 tacgtaaaat atttttaaaa ataatcaaaa atcttaattt tcaatattga atacaattaa
      301 attttcaaac tcatctaggg catgacatac aaaagcaaaa aacattaatt tagtatctga
      361 taaaaattta aaaattgtaa aagggggaag tctgttattt ttcagaagca atgtaattgt
      421 aaaaatataa atcagaatat tttgaaaata tgggggataa acttatgatg gatttagaaa
      481 aggaaaaaca tgaaataatt gaaattataa aaaaattaaa tattgataaa gataaaagga
      541 atttattgat gataaaaaat tatatccttg gacttttaaa aaaatagaag aatagcaatt
      601 gctattcttc tatttctagt aaggaatcta tcattttatc aaatacagtc agatattttt
      661 tattcaattt gaacatttta gttataattt ctctttctac ttcattttcc attttactta
      721 gttcaccaac aagatgggca aattgctcat ctaattcttt gataaaaaac atctctccaa
      781 ctccatgctt aagccattct tcagatatgc tccaagtctt gcaaatgtcg ttcaaagttc
      841 tttcgcttgc atttctttta ccattttcaa ttaaagatat tgtgtttctt tttaaattta
      901 gtttattagc aaactcttct tggcttaaat tgagatttaa tcttagttct cttattcttt
      961 tgttttctgg tttcatttca tctatcatta ttatccctcc atatgaaata ttatatttta
     1021 aaagtgctta caagtcaaca aaaataaaag aaaaaggtat tacaaaatag ttgacttgta
     1081 agcagacatg taatataatt gtattacaca atcaacaaaa gcgaggtgat tgaaaatgac
     1141 tgatataaat aaaattagag aagaaatata tagaaaattg gagttacttg gccctgcaaa
     1201 actaaaatta gcattgacta aagtagatat attaaaagaa atacaagaca ttgaagatga
     1261 aaataatata caaaattaaa ggggtgaaaa gattgaaaat ctcagatttt aataaaaatc
     1321 aaataggatc agcagttcaa ttacaagata taaggtttga tacaaaagct gattgcatca
     1381 ttacgaaagt agaaaagaat gaaatactag tcatgtatta tgaaaaagaa acagaggaaa
     1441 tagcatataa aactttgact aaagaagatc taatacttga tgactataaa cttaagttat
     1501 tatcttaaag taatttcaga agggaggtga taactttgga tcttttgaag gatgcaagaa
     1561 aaaaagagtg gttttggctg gaaaatgatt tgattgatag agaagatttg agcatctatg
     1621 aaaaaatgat ttacattgtc ttagctagat atagtaacga agaaagttct tgtttcccaa
     1681 gttataaaac aatatcagaa aaagcaggtt gcagtataag gcaagtatct aatattttga
     1741 atgaattaga gaataaggaa ctaataataa gagaaaatcg taaccacgaa ggcaaaaaag
     1801 agaaaaacag taacttatat ttcttaatat cttcaaaagc aaaggtacag aatgaagtgc
     1861 ctaacgttag gcatgaggtg cctaacccta tggcaccagg tgccttacat gttaggcatg
     1921 aggtgcctag taaaaagact tataataaaa agacttatat aaaaagtaat accactacac
     1981 aaaaagaatc taaaactgat tatttagacc tatcattctt ggacttagat atagaaaaag
     2041 taaaactaac taaagatgaa tatgacaaac ttataagcaa gtttgggaag aaatacatac
     2101 acgataaaat tgttagttta gaaaactata tcataaatgg taaagggagt aggtataaga
     2161 gccactatag ggctttgctg acttggggga atggagatgc tagtaaaggt atagtaccag
     2221 aagttacaaa gactaagaat cctcttagtg gatttaaaga actttaaaaa ctaaatagta
     2281 aggaggtaga taggatgaat gaaaaagaag agactgttta tttgaatagt tctaatgtga
     2341 agttattctt aggcgttagg gatattaagg aatttgagga attgatagaa aatgtcaatg
     2401 agcgaatttg tcaactccaa gaatcgatta ataaacttgc ccaatttaat attgagttcg
     2461 atattaagtc agatatttag ttaagtataa attaggaggt tgcctatgaa agatgaaaag
     2521 ttagaactta ctgaaaaaga tttacactgt attgcaagac atttacaaaa tgaagttatg
     2581 gagatggtgt ttagagggaa tatagaagcc cctacatcat gcgaagtttg cgattacttg
     2641 caagaatgca aggactattt cacccatata gacactttta taaaattaag tgaaatgaca
     2701 ggtgtagata tttttactaa ataaatttag taaattaaaa ttttagtatt gtaattgacg
     2761 tgctttttta aaaagaggac agttgctatt agcagtcatt atttgaattt tgcaaatgta
     2821 atttttatta cattcgcatt ctaaaagttt tgatccagtg aatccaggaa aagttttata
     2881 ttcaccagaa aattcaactt tatcgtcaat aagagagcat ttttctctta aggttttaat
     2941 tttgcacata ttcatcacca ccctataatt aatttgaaca tactgtcaat atgctccgat
     3001 taattataac atgcaaatta tcatgatgta gaattaaagc ataggtcaat taaatttagt
     3061 tataaaatca atgaaacaga taaataatta aatgttaaat aaattagggg gttgttcatg
     3121 aaagaggaaa agttagaact tactgaaaaa gaattgcatt gtattgcaag acatttgcaa
     3181 aatgaagtta tggagatggt gtttagaggg aatatagaag cccccacatc atgtgaagtt
     3241 tgcgattacc tgcaagaatg tgagggagat tttacctgta tgtatcattc ttttactaaa
     3301 ttaagcaaaa taacaggtgt aaaagtgact gcatttaagt attgttttcc agatgaaaat
     3361 cccaaagcta ctatctaaga agttttttta gaataggaca ttcttcatta gaagaagagt
     3421 cgcaaatatg acttccatca catctagttg acattaactt agatccaaaa gtacctggag
     3481 gagctgaata ttcgcaagta agagaaatgg tttcgtttat tacaggacat tctttgctta
     3541 taactcgata ttggctcata ctctcaccac ctttctaatt aatttaaaca tattggaagt
     3601 atgtttaaat taattatagc atgtaaatta tcatggtgta gaatagtttc aaatcaaaag
     3661 aagcaactaa atagttaaag acaggaggta tgctaagtgg aaagaaaaaa acatgaattt
     3721 acagatcaag atatacaaag acttattgaa ggattaagaa ctgatccaaa aataagagaa
     3781 atgatattta aaagtaaagg ttgtgaaact agaacaagta caagaagtaa atgcgaacaa
     3841 tttgttgaag aaaaacttgc ttatagaagt tatggatttg aaactccaaa atactatgca
     3901 aaacttattg ccgacataat tgatatttta gtttacaaaa atgttccaag agatcgtatt
     3961 tgtggggtat tgtatgacgt cgaaagggtg attcctttag ttgtgacatt ttaataataa
     4021 gctgggggtg attaaatgaa ttactcttta gaagcagagc aaaacattct aggaagtttt
     4081 cttataaatg actctatagg ttacaaaata agggaattaa aagaaaatga tttctacttt
     4141 gagtcacata aaattatttt atcttgcatg aagaaaataa ttgactcaaa taaaccacta
     4201 gatcttttgc ttttaaaaaa tgaattagaa aaaatagata gattagtaga tattggaggc
     4261 gttagttaca ttaccagttt aacaactata gttataacta cttcaaacat agatcactat
     4321 ataaaaatca taaaagagaa gttacttaaa agacaaattt cagagttagc aaatgaatta
     4381 attaataatt caaattcaga tacaagtata gatgaattga ttatagatat aaacgattta
     4441 aaagcacttg tgacgacgag cagtagtgta aatgataatt acattgacgc atcaaaaata
     4501 aaacgtgaaa aaggcgtaca caagtccata gacacgggtt ttaataaact aaacaatatg
     4561 ctagatggat ttagatatgg aactcttaca attttaactg gtaaacctgc atctggtaaa
     4621 tcaactatag ttaaccagtt tatagcacaa gcaattacga atggagagaa agcatttttg
     4681 tattctggag aattaccctc tttcatgtct atggattggt ttagaaaaac tgtagctaat
     4741 gattatcaca taaaagaata taaaagtgtc tatggaggaa catacacaga tattccagat
     4801 tacgccgttg agcttatatc agactggata gaagataaat tttttctcta tgatgaagat
     4861 gcaataagtg atgaagtaaa tctactaaat accatagagc atttatattt aaaaaaaggg
     4921 gttaggttct ttgttcttga caatctaatg acaataaaaa ctggcaataa ggcagataaa
     4981 tatgagagac aagagcagat agtaagcaac ttaaaaaatc tcgctaaaaa atataactta
     5041 gtaatagttt tagttgcaca tcctcgtaaa aatatgggtg acatgaagcc aactatgtat
     5101 gatgtgtctg gagcaagtga gattgttaat tatgcagatt atattttatc aacttacaga
     5161 gttgttgatg aagaagaaga gacagacgac acttatttat taattttaaa aaatagaata
     5221 acaggaaaac agaatataag ttttaaaatg aatttttcag aaagaagaaa aagactttac
     5281 acgactagtg aggagttaaa cagagattac aaatatgatg taaataaaca atatgttcaa
     5341 gtagaaattc cagaagatgt attttaaaaa aagtataaaa aataggaaat tttattaagt
     5401 aatgatttta taagaaaaac caatattgaa taatttaaaa attcaatatt gaaaaaaatc
     5461 atttttcaat attgaaaaac ggattttgaa aaagtattga atttcaatac taaaaaaagt
     5521 ttgaaaatat agcatttaca aggtttatat aaattaattt cctgacaatg tacacacagg
     5581 tttagatatg ctagcaaaaa aacttaaaag tagtgtaagg ataaaaaggc tatataaatt
     5641 tataaaaaga tttaaaagtg ttattaaatt agagctaaag tttattaatt aatttcctcg
     5701 tagaaaaata ttattttaaa taaataaagg agtgatttta catgatagca ttagaaaaat
     5761 ttgcaggtgg agttctaaaa gaaaagttta atacagagtt acaaaaagtt ttagataaca
     5821 ttgcagatcc aaatacagat tttaaaaaaa caagaaaaat tagtttagag atagtgttta
     5881 aagcaaatga agatagagat cttgctgaag tagatataaa atccaaagca actatagtag
     5941 aagcaaaggc tacaacaact aaagttctga taggaaaaga tttagagaca ggaagagttg
     6001 aagcgtctga atttaaaaat caagtagcag ggcagttggt catggatatt ccagatagtg
     6061 aagaaactaa tgaaacagaa gaaaatagtg ctggtgtaat tgatttcaga aatgcaagtt
     6121 caaagtagtt taagaaagta agtaaaatat aaatttaaat aaaaaaggag aataaaaaaa
     6181 atgataagaa atgcaatgga atatttagtt aagttaggaa aaagagaaac agtatgtgta
     6241 gatgggttta agtacacaac agatgcatta gaaataataa aagaaccaaa agctaatgaa
     6301 ttagaaataa ctacattgag tggtttagta gattatataa aaagtggcat agatcataag
     6361 gcagatgagt tgttattaat acaagttcta actccaagaa aagtgctttt aaaatctcct
     6421 ttgagaaaaa atagagatag agagacatat attgaatgtg ttgcactact accagagaaa
     6481 agatttgaca gacctttaga cttagatagt tttaacataa tgctacaatc agcatttata
     6541 aagaataatg atagagatac acttttaaaa attgcaggaa atatccaaga atctacagta
     6601 aaaaatgtgg gagatgatgg agtttctcag tctgtaacaa taaaaacagg cgtagctagt
     6661 gtatcagaag caatagtccc aaacagagta aagttaattc cttatcgcac tttccaagag
     6721 atagagcaac cagaaagtga gtttattttc agaatgagtt ctggaccagc agcaggatta
     6781 tatgaagcag atggtggagc atggaaaaat caagcaatgt taaacattaa agcgtacttg
     6841 caagaagaat taaaagattt taaaaatgta aatataattg cttaattgaa aatctaagag
     6901 ggcttacaat aagtcctctt agtaaaaaag gaggggatta gatgaaaatg atttctcata
     6961 tgaacatttt agaaagagca gaatttacag ataaaatagc taatgcaatc atgtctgcaa
     7021 ataaagaaat tgaaagaggc tgtgccttta atgaagcagt tgagattgta aaacaaatgg
     7081 aatatagaga aaataaatac aatgaaggag atattgcaat atgaataatg tagttttagt
     7141 tggaagatta gcaagagatc cagaattaag atatatacca gagtatggta ctccagttgc
     7201 tacttttgca ttggcagttg ataggggtta tacaaaaaag gatggaacta gagaagtcga
     7261 ttttatacca attgaagtta tgggaggatc cgcagaattt tgtgctaatt atctcgcaaa
     7321 aggaagaatg gtttcaattc aaggccaaat aaggatagaa aaatatgaaa aagatggaga
     7381 gaaaaaaact ttcacaaaag taagaacaaa aattgttaat gcgcttgatc gcaagccaaa
     7441 ggatgaagaa aaagaaatta gttttcaagt tttagatgat gaagatattc cattttaaat
     7501 tggatggtga ttatgtgaga agtttagagg agagaaataa attagttgaa gaaaatataa
     7561 attttgtatg gtttattata agaagatatt tcaaagtatt tatttcaaaa tatccatatc
     7621 ttcaagatga tacattttca gaaggatgta ttggattatg taaagcagca gattattttg
     7681 atagtaaaaa aggtattgaa tttaccactt ttgcagctgt gtgtataaaa ggtcaaatat
     7741 ttaaatttat aagtaggtat gttaaaaaac attacagagc agatatagat agttttgaaa
     7801 aatgcatcta taaaaatact catggtgaag aaataagaat agaagataga ttggctggta
     7861 atgaaaatgt agatatagaa aatgttcatg ttattagatc ttgtataaaa agaagtaaaa
     7921 tagaagatat acagaaaata acatacttaa gggaaaaagg atacactcaa aaagaaatag
     7981 gcaaaatact taggactaac caaatgacaa tatgcaggaa gcttggaaaa ttaaaatctg
     8041 aaattaatat actaggaaaa taatataata atttaagttc attggaggca aaatgaagac
     8101 taaaataatt ggtggtaggg aaaatcagtg cccactttgt aatggtagtg tatttaagat
     8161 tgaaacatca attggaatag tatggcaatg taaggattgt ggatgtatgt atcaagatat
     8221 gaattgtaaa gaaaacaaaa ggaggcatta agtatggctt gtgttataaa gtgtgatttt
     8281 tgtggtgaaa caatagcaga aattctaaac ataaaggtat tcagaattga tgatgaaggt
     8341 aatataatga gtatcggaaa agatgtatgt gacaaatgtt atgacagatt atttagaagt
     8401 aacttgaata agaagatgac taattatgaa aagataaaaa atatgagcaa aatagaaatg
     8461 gctgaatttc ttgcaaatgg cggaagtgga tgtactagtt gtgcttatga ttttcaagaa
     8521 tgcttaggcg gatgtttaga aggtagaaaa aaatggcttg aaagtgaggc gatgaaataa
     8581 tgaaaaagac gttaataatc gtaggagtag ttatttcaat attaattatg tttgcttgct
     8641 tgttcttagt aactaaaaat aaagcaattg atttagaaga gcaggttcaa tcagcaactg
     8701 catctgtaga ggtgcaagaa aaaaggagaa ttgatttaat taagaacata gtagactgtg
     8761 taaaagacta tgctaaatat gaaaatagaa cattaaaaga agtaactgac atgagagtaa
     8821 gtgcaaatga tgaatctatc aaggaagcaa aattaaatat acaagcagta gcagagaagt
     8881 acccagattt aaaagctaat gaaaattata agcaactaat gagtgagttg agtataacag
     8941 aaaatatgat tgcacaacat agagaaaatt ataatatgca agttcgcaat tacaataaat
     9001 atataagaaa atttccagca aatttaattc taaatagtat gggatatgaa aaaatagaat
     9061 ctaaatatac tgaatataaa gcaaaagagg atgcacctca aaatttattt gattaagagg
     9121 tgcacatatg aaatttaaaa cttttgaaat aactaagaga gaatttttag tgagcataat
     9181 aattgcttgc atgatgattg tgcttggatt tttcattgat gataaagttc aatcaaaatt
     9241 tttagaagat aatgaaagat attacaaagc aactaaaata aataatgaca aagatctctt
     9301 tgattatgct atggaaacaa gtgcgggaga ttctttgata agtggaagat ttaaaagtgt
     9361 agattctgta tctatagaag aattaaaagg ggactattgg tttatagaaa gaacggaaga
     9421 aaaatacact atgcatacta gagtggttac cactaaagac agtaaaggcc attcacataa
     9481 atatactgaa acatattgga cttgggatta ccatgattca atagagaaaa aatgtaataa
     9541 atttaatttc atgggtaaag agtttgatgc gaaagtaatt actaatttat ctaaagagag
     9601 attggcatta aagaaagata tatttaaaaa taataatctt aaaataaaag gtaattatgc
     9661 ttacattaat aataagagaa ggtactatta tgaagttata ccgaaagaat ttaatggtac
     9721 tatgtatgct aatttaaaaa ataatactat agcgaatcca gctgataaaa acataaaagt
     9781 ttttaagggt gagtctatac agaatgtttt agaaaataat aattttaata cagggttttt
     9841 taaagtagtg ttttggatca tctggatatt aataactaca atcattatat atgtatttta
     9901 ttatttaaaa aatgattggc ttgaagataa ataaagatgt aagaggagaa gatcaggtat
     9961 gaaatgtaag gtgtaccatg ctattaaaaa tataaaagat gcaaatgagt taattgatag
    10021 atttgaaacg gaaaatatat tctttgaatt aaatgacata gataatgaaa atgtcttaaa
    10081 tggagaaatg aatttaactg atatagaaga tattgcatca ttgcaattta tattagatgg
    10141 agatttattt atatttagta gttgtgatga aatgatattt atgaataaat acactcaaaa
    10201 agagttggga cttcaataaa tgaatgaggg gagatactaa agatggattt aaagttaatt
    10261 agcagtatac ctaattggaa ggtagaagat cagttggcaa aaattgatga agaggtaact
    10321 gagtttaaaa atgctataga tacaggaata actaaagata taattgcaga aggattagat
    10381 gtttgtcaaa ctattttaac aatgtttcaa gttttagaaa tagaaaacta tattactgaa
    10441 ggagtagaga cacataataa gaaactaaat ggaagaggat gggatctcaa agaaatagga
    10501 gacgatatac ttaatttaat taaaaattca atgattaatg aattgattaa tatgaacaag
    10561 aaattaaaat gccttgatga aaaagaattt aatgcaaaaa tacaattttt agatgtagat
    10621 aaactatgga atttgtgcat gcctttagtt aacttaagta ctataaaagg agttaatgaa
    10681 agcattgatg aaatgaaaat gggagagttt ctaaaaaata ttaattaaag taaggagata
    10741 atataatatg ttgatagaag taactgaaaa taatagagaa ttaaaagtag gagatattgt
    10801 tgaaattaat ggtggtaata agttatatat gattctttct ttaaaggaaa caataggtgg
    10861 gtatgttatt ataaatatgc agaatggata tggttcattt ggtgcttata aaagtttaag
    10921 tcaactagaa ttagacttga aggtaagagg ttataaatta tatagttctg atgattacaa
    10981 gttgcaatta gtgcctagag aataagatat atttcaaaaa agaaaaaaag gagcattact
    11041 tcacgctcct gcttgtcaaa attctaaaac cgtcatcaca acatcattat aacataatta
    11101 ggagtgtgag gtaatgcaat ctagtaaaaa agacaatttg tttagtagtg cagaaggtaa
    11161 gttatatgat tataaaaaaa taaaagcgga aatagaaaaa atagacattg atatgcagat
    11221 gataaaaaac gactacagag gatgtagtgg agttgaaatt aaagaaaaga caggcaagac
    11281 atatgatatt aaaagtgtaa ttgaaataga gactgaagaa aaagaaaaga aaattgcctt
    11341 aaaagaaaaa gagaagagac ataaagagtt gataatagaa aaaatagata atgcaatgaa
    11401 aatattaagt gaagaagaaa aaaagatagt acagtataaa tacttttcaa gtagcaggac
    11461 atcttgggaa tatgttggca gaatgattgg tttttcagcg agtaagtgta agcaaatgag
    11521 gtttgatatt atagataaga taaaaggatt gctataaaaa tgtccgattt gtgacgattt
    11581 taaaccgatt tatgtctgtt ttgtgtctaa aaaatgtccg gtttgtgacc aatttatgtc
    11641 tgttttgtgt cttttaatca tgataagatt gtattgtgag aaaaatatat ttaataaaaa
    11701 tggcttggaa tttacgttct aagtcttttt tattgtctag agaggtgttg aagattaaac
    11761 gtgatataac agaagaaatt tcaagtgctg catatgtacc agacaatcta aagtattacg
    11821 acaatgtaat gaaagaaact cctggtatgt atggaagaga atgttcttta gattttataa
    11881 agaaaaagca agaaaaactc ttgaaactaa aaaagaaggt taagaaaaat tatgattcta
    11941 atattaataa gatagactca tatctaaaaa ttttagagaa aagtataatt gatgagtctg
    12001 atcacgttga gttggttgta tttaaattat atttgcaatt agaaaatgtt gtaaaagtaa
    12061 ctgaaactgt aaacgactta ggatttagaa taaagactaa cacttatgca agagaaagaa
    12121 aatatagtac aaatgatata acctctataa tcacagatcc ttttactgat gtagttgagg
    12181 acttaaaaat attagttcaa gagaggcaaa gaaaaaatta ttatggaaat aaggagtgtt
    12241 tttaatgaat ggaagcacat agtggacaaa aggttaatcc cataaaagat atagaggatg
    12301 tatttagact tttaaatttt ctagaagaat ggaatgaaag aaactattta ttagccctct
    12361 ttggaatgtg tactggactt agggtagggg atatattagc acttaaagtt gcagatgtaa
    12421 cagacttaaa actcgataaa aaaggaaata aaataagagt atctaaggat tggattagag
    12481 ttatcgaaga aaaaagagac tataatagag aggttttttt atctgatgta ataaaaagtg
    12541 ctatagaaaa ttatactcaa gataaaccag gagaagaatt tttatttaaa agtggcaaga
    12601 ggaagaaatt taatagacct atccaaacaa gacaagcagg aagaataata aaaggagcag
    12661 caaaaaaggt aggaataaaa gaaaatgttg caacacactc tttaagaaaa acatttgcaa
    12721 ggcatattta tgatgaagaa gagaataaga cttatgcatt agaacttata aggaagattc
    12781 taggacataa gacaatagaa atgactagaa gatatatagg aatagataag gatgaagaag
    12841 taaaagcaat aactaaattt actaataagt taaagaaaag aaaaagaaat taaaatatct
    12901 tgtattttat ctaaaattta atatgttacg tttttcaaca acaggacatt attagaatgc
    12961 aaagagcctt aacaatagag tttacaagga atagaataga tttattaaaa tgtctgaaaa
    13021 tatatattaa aggtcattta ttcaagggat ttatagggat gtagatattt aaatgtttat
    13081 ttagaagctg agtaaagagg tgattaattg gatgtgaatg aagttgaaat aatcgatagt
    13141 agctatgaaa aaggtaaaga gttgacagaa gatcagtgca atatggtaac tctattgatt
    13201 acaggtgcta cagtaacaga aactgccaga attattggag taaatagaaa aacaatctat
    13261 aattggatga ataaagagca tgttaggaaa gaaatggaca gacggaaaca ggagctaaca
    13321 aaccaaggaa atttaatgat attaaaggat ttagatagtt atatcaacaa tatcaaggaa
    13381 ttagctagtg acaagagcga taagagggtt atgttggcag ccaatcagta tcttataaat
    13441 cgtatatatg gaactcctac aagtacagtt atacaagcag aggataatag tgttggcatg
    13501 ggtatggatg caacagagat agaagcagct atagctaaga taagaataag aacaagcaag
    13561 aagtaattag atatgaaata aatcctgcaa gaatatgttt taattatgtt tgaaatcaat
    13621 taaagcaaaa catatattat atatatattt actttaataa ttaaatcata tattgtattt
    13681 aactttagtt atagctaatt taataaaatt tagtgagact taataattaa agtttcatac
    13741 agtgaagtga tgtggattat tataggatat gtgaacctat tggaaatgct aggttctttt
    13801 taactatgcc gaaaatacat atttaaggaa tagttgaaag ataattaata attctaatca
    13861 aaaaaatatt tcttgcaaga aaataattca aaatacttta atatatttat tttttttatt
    13921 aatagggggt gtccttctat tttgaaaaaa ttatttttta tggaaaatga gttctagaaa
    13981 ttttctagca aaatttaaaa accagagatg tatttcaaag agggtgattt tattagagaa
    14041 aaatttacat tgtttatttt attaattgca ttactcactg agcctaaaaa aatagaagaa
    14101 gtaagaggaa gtaaatacga gtggataatc tagaaagaaa tatacaatta atatttagtt
    14161 atctagcaaa gacttttata agaaatggag catctataaa agatgctgaa aaagaagcaa
    14221 atgagattat aaaagccaat tcaaacaatc tttggggagt taatggtctc gcttatcaac
    14281 ttggaaaaat aaacctagaa tttttctgta tgtattttat gcaagacaca tatttaccaa
    14341 aagaagataa tgctgctgct cctatagcta aagttcatca tgagctatgg gaagatatac
    14401 aagaatctat aattggagat ggttcacagc aattaggaag aatttatcca agagggacag
    14461 gtaaaagtgc atttggagat ttagcgacaa ctgtatggtc gcattgctat aaacataaga
    14521 catatacttt gatttgctct gacataggat ctacagcaga gaaatttgta aaagatataa
    14581 aaaatgcatt acttgaaaat gagtatatta aaaaagcatt tggtgtcctt ttaaatgata
    14641 atgatagaaa atacatttgt aatagtactc aactagagct aacaaataaa acttttattg
    14701 aagctatatc atcttcatca cccatgagag gaagaaaata caataataac cgtccagatc
    14761 ttatcatact tgatgattat cagtcagaag aaaatgttag aacagaagat gctagagaga
    14821 aaaaatttaa aagattttct gatgatgtaa aatatgctgc tcaaaaacca gtcataagaa
    14881 atggtaaaac tataaaaaga ggaacaactt ttatagcatt aggaacctta caacacaaag
    14941 aatgttttta tagtagacta aaaaatttac caacttggaa gtttaaatgc gaaaaaggag
    15001 ttttagttga taatgtagat gaattattta attctggttt atggcttaag ttcaaagaga
    15061 tcttatttga ttttaaaaat acaaatcatc ttgaagatgc taaagaattt tactgggaaa
    15121 atgaaaaaga aatgaaattc cctatattat ggccaagttt ttgggattgt ttagatatgg
    15181 ctctaagtta ttatgaaaat ccaacatctt ttaagcagga agtgcaaaat gatgtcaatt
    15241 ctataggaga aaaatggttt aaaactgtta gaacagaaac tagagaagaa attgaatcgc
    15301 atacttttaa aaagacaatg ctgttatgtg atccagcatc tgcgggtggg tcgaaacatg
    15361 actatagcgc ttttcttgtt ggaagtgaat cagaaaatgg tttactatat ggaagattgg
    15421 cagaactagc taaaataaat gctagaactg attttgataa atatatagac catatgattt
    15481 atttattaaa agtgtattca gatataacac atatttatat agaaaaaaat acatttaatg
    15541 gcgcagatgc caaccaatta gagtttaaaa taaaaaatga taatgttctt agttatagag
    15601 atatagaaat cattaatgag caacaaaaga aaaataaaga tgataaaatc tctactttaa
    15661 tacctgtttt aagcaaaggc caaatgattt ttgcagaaga agataaggaa tttatacagc
    15721 aaatacttga ttttacagga caaaaatact ctctacatga tgatgctccg gatatatcat
    15781 cggaatttat taatcgaatt aacaacataa aagtttctga gacagtaaat atctttgata
    15841 gaaaattatt aggtttttag ttgggaggta atttattggc taatagatca aagtttgaag
    15901 aagttcaaaa aactttaact aaaaaaggaa agaagttcaa tgttggaata ggaaaagatg
    15961 aaaaaggata ttttgcatat actcatagag caaggagtaa aagttatatt tcaaagcaag
    16021 atattccaat aaaagtttta aaatttatag agtctacagg ataggatgtg ttgttaatgg
    16081 aattagattt agacttaata aaagacatat atgacgattg gaactctaat aaatccgaat
    16141 atgatactat gtataagtat tataaaggtg aaacggatgc tattagcaac tataaaatgg
    16201 ttaccaagag atctaataat aagataaaca ctaatttctt aaaaaagttt attaatgaag
    16261 aagtcgctta ttctcttgca aataagatta cctatacaag tagatctggt gatgagaaga
    16321 ttataaatga tttagaatac tatacttgcc attggagtaa aaaacatgac tcagatttat
    16381 tgaggtatgc actattattt gggctttgct atgaacttta ctatgtaaaa gatgatgaaa
    16441 tacaagtaaa gataatcaag ccaacagatg gctgtcatta tgaaaatgaa aatggagaaa
    16501 tagtatgctt tattagagag tttaccaaag gtttcgaaga tgacacgtat gtagatgtat
    16561 atgacaaaga atatatatat cattttgatt caaatttcaa agaagttgaa agtcctacag
    16621 taaacaatat atttgatgga aatgttccaa tatcaatttg caaaagaagt gaagaactag
    16681 gaaaaaatac aatatttaat gatataaaag ggttacaaga tgcttatgaa actaatttaa
    16741 gtgatataag taatgaaatt agtgatttta gaaatgctta tttaaccttt tctggatgta
    16801 atattaaaga agatgatttg ccaaggatga aagaacttgg aatacttcaa gttaatggag
    16861 atggaaaaat agagtggctt attaaagata tgaatgatac atttgttcaa aatacactat
    16921 cttcaataaa agaaaatatg tatgaaatta catctcatat aaatcacaat gaaaaaatgc
    16981 aaagtaatac atcaagtcta gcagttatag caagattaat cagtacagaa tggatttgta
    17041 gccaaaataa tgatagtatt gcagatactt tatttaatag gtacaaatta ttgtgcattt
    17101 ggcttaataa aaaatatggt tttgactatg attataaaga tattaaggct aaatttaccc
    17161 ctaagatacc acgtgatgat ttagttgtag caaatatatt aagccaactt ggagataaat
    17221 tatctactga aactggctta tctcaactaa gtttcattga taatcctcat gctgaaatag
    17281 aaaaagctaa aaaagaacag gaaatagttt cagagggaga gattttatta gatgaatcaa
    17341 aagattataa ctaaattgac tgaagatata tataaatctg ctgaaaatag gacaaaacca
    17401 ttttataagt atcaaagaga aaatagggat aaacttttat tagaaatagc aaagatattg
    17461 ctaacacacg atattattaa tgaatactta aatattaata ataaagataa gaaaaatcta
    17521 aggtcaaaac ttaataaaat tataaatgag tattctaata gttatgatga agaagtcaag
    17581 gatatagaaa ctattttatc tgattcatct aagaataaat attcaaattt gataatttta
    17641 ttaggagtaa tactgaagat aaaagatgtt agtaatataa acaataaagt tataaataag
    17701 atagtgaata ataaggttga tggaaaacat tggagcgata gactctggaa gaataaaaaa
    17761 ggtatagaag agtccttaaa aaaagaaata aagagttttc taaatggaaa aacttctgta
    17821 aataagatag aaaaagcaat aagagataaa tattcaacag gtgcaggaca aaccagcaga
    17881 ctaatagaaa atgaagttgc cagatgtcaa agtgaagtaa atgagtattt tttcaaggtt
    17941 caagggatta taaaagtaat gtattgtgca gatttagatt ctcgcacatg tagtgattgt
    18001 tctatgcata atggagtgat attttatgtc aatgaagata ggccctcttt gccaagacac
    18061 tgtcactgta gatgtcaata tatacctgtt taacatgtat taattaattt aataggtgtt
    18121 tttattatgt ctaaaaaata tgaggtgaaa tatgaaagtt gaaatattag gaacagaata
    18181 tctagtgata gagaaacttg aaagcgaaga tgtgctatta aaggaaagag ctggatattg
    18241 tgatcactct gttaaggaaa ttgttataga gaaaataaat tctgaagaag gttcattgaa
    18301 agatttaagt gtatataaaa atgaagttgc taggcatgaa ataattcatg catttcttag
    18361 tgaaagtgga ttgaagagtt gtagttcttg ggctacaaac gaagaaatga ttgacttctt
    18421 tgcaattcaa tttcctaaaa tagtaaaggt tatggataaa atagaatgtt tgaattaaat
    18481 tttcaaaaag gagaacttta tacatgttaa aaaaagaagt gttggaatta ctaaaagaaa
    18541 tagaagatga tgcagaaata gattcattgc tgcaatcaac agatttattc aaatctgcat
    18601 ctgataaaaa gcttacaata gaagagttta gagaattaat taataatgat gcaaacttta
    18661 aagctattat agataatgaa aaaaataaat atcacagcga agcacttgaa aactttaaga
    18721 aaaaagatat gcagacactt ataagtgctg aagttctaaa aagaactgga gcaaatgaaa
    18781 cagaagagca aaaagcaata agggaattaa gagaaagcct agacaaacta aaaaaagaaa
    18841 agcagtacgc agaaaaagtt tctaaatata aagatatttt agttgaaaag aaaataccta
    18901 caaacttaat tgaatatcta ttatctgatg atgatgataa aacaaatgca aatatagaga
    18961 tatttgaaaa ttcaatgaag caatatgtgc agtcaaaggt agatgaaaga attaaagatg
    19021 gttcatacgt tccacctggc aaagatagtg caggttcgct atcagaaatt agaaaacaaa
    19081 taaagcaagg gctaaatagt ctttagtata aaaaaagaaa ggatgatttt ataaatggct
    19141 aatgtattac aatatgtaac actttttcaa caagaactag atatgcaggt aacagttggc
    19201 tcaactactg gatggatgga gaaaaatgct ggtcaagtta tatataatgg aggaagagag
    19261 gtaaaagtac caaaagtaac aatggatggc ttaggtgatt atgacagatc taaaggatat
    19321 acgcaaggcg cagttacact tgagtacgaa acaatgaaaa tggagatgga tagaggtagg
    19381 acttttatgt tagattcaat ggatgtaaat gaaagtaact tcgttataaa tgcatcttct
    19441 gtaatgggat tatttcaaaa gcaacatgtt ataccagaaa tagatgcata tagatactca
    19501 aagatatttt ctgaaacagc taaagtagat gagcgtgtaa aatattcata cgaagctaca
    19561 gtaactacaa tattaagtga attgaaaaat caacttgcat caatacaaga tttatctgga
    19621 gatatacctc ttattattag tatgtctagc ataattgcaa gcatattaga acaatcatct
    19681 caacttgatg atattgaatt tggcgatgga gatataaaaa gaattgtaaa gtctatagac
    19741 aatgtaccta taataaaagt tccatcaaaa agaatgaaaa cagaatacgt atttagagat
    19801 ggtaaaacat ctggacaaac agagggtggg tttactgaag gagcaaaggc tttagatata
    19861 aactggttaa taactccagc atctgctcca attgcagtat ctaagactga taagccaaga
    19921 atatttactc cagatcaaaa ccaaacagca gatgcttatc aaatagacta tagaaaatat
    19981 cacgatttgt ggatattgga tgaatattta aaactttgta gagcatctgt gagacaagct
    20041 aaagctgcac aagaataagg agttgtttta aatgtttgca ttgataaaaa ataacataat
    20101 taaagttgtt gaagatgaaa attcgagaga taaattgatt tcaaaaggat ttaaaatata
    20161 ttcagaagaa gtgactaaag agaatattga gaaaaaagaa gaaaaagtta aatcaacctc
    20221 taatgattta tttaatctat ctaaaccaga gcttatggaa attgctaaag gcaagaatat
    20281 aaagggatat tcaactaaaa ctaaagagga attaattcaa ctactagatg agtacaatgc
    20341 ttgataatgt taaattagtt ttaggaatag aaaatgatag atatgacaaa ttaataatcc
    20401 tttatataaa taaaataagt gagatggttt tagaatattg ttctaggcag gaactaagta
    20461 cagcactaga agggtttgta gaagaaaaag taatatcaat attatcttca agatttgaag
    20521 aacttaagaa aaatagtgga cttgataatc tagaaaaaag cttaaaagct ataactagag
    20581 gtgacactaa gatagaatat aatgctttaa cattacaaac tgctgaaaat agcatcagtt
    20641 catcatcttt tttaactgat actgataaaa aatttctaag cccattttgt attgttagta
    20701 tgtactaagg ggtgattaaa tgactgaagc agatatatta gaaatgacat accttgataa
    20761 aatgactata attagaaaat taaaaggctg gaatgaagaa acatgttcta atgattttag
    20821 taatgttgtt atagctgaaa atatcccatg tgcaatttct agaaaagaag aagccattgt
    20881 aaatggtgat attggatcta tagttgtaaa tagaaagtta ttttgtagac cagaagtaga
    20941 tataaaaata ggtgatacta taaatacaac ctataatcat ggagaagaag ctgtatttaa
    21001 ggtttctaag gcaaatatat atccaagcca tatggaaata cctattacag aattggagag
    21061 gatataatgg gaatagataa tttaggagtc tttgaagcat tagccttaga tttaggtcta
    21121 aatgcatcaa actttaatag aaaaatcaag aagtttacaa aagaaactgg gcaagaacta
    21181 gcagaaaata taagagatga aactcctgta aaaactgaaa agttaaaaaa gggatgggaa
    21241 gaaaagcaac cagaatatga tacttatatt gttaaaaatg aagttgatta tgctacttta
    21301 gtaaataatg gccattcaac taaaaatggt ggcattgtcc ctggttcttt tatggttgaa
    21361 aagggaattg aaagaacaaa agaaaatatg acaagtgcaa aagcaaattt atttgattgg
    21421 taggtgatta gttgcttaca aacagagaca ttataatagc agttaataaa agaatcgaaa
    21481 atgagtttag atatatagat atttttatta atgaggataa tatacaaggc tttgaggagg
    21541 cttgtttttt tgtgcaactt ttacctgttt ctaataatat tgcaaataaa gttttagata
    21601 ctaaaaagat attcatagat attgaatatt accaaaaggc tagaaaaaat aaattaaatc
    21661 tatacgacat acaaagtaga ttagaaaaaa tatttaacag aaacattaga gttaaagata
    21721 gaagtttaat cattgagaat atatctccat caatcgcaaa agatcctgta ggatataaac
    21781 taacttttct aatgactgta tcttattttg aagaagtcta ttttgaaaat gaaacatttg
    21841 aaattatgga aagtgtagat ttagagatag gaagtgatta aatgggttta ccagaaatta
    21901 atatttcttt tatccaagca ggaaaaactt ttgttaaacg ctctggtggt attgtagcgc
    21961 ttattttaaa agatacaaaa aatatagggt tagttgagat aaaagaaata gaggatattc
    22021 cagaaaatta ttctgctgct aatttagatt atataaaaat ggctatgaag ggaaatgtat
    22081 atcctccaaa taaagtatta gtttatacat tagatacaga tgaatcaata gatgatgcac
    22141 tagattttct tgaaacttgt gagtttaatt atctttgtat gccagatgag acagaacaag
    22201 atttgcctaa aatcaaaacg tggattaaga aaatgagaga agataataaa ataaaggtta
    22261 aagctattac tgcaagtaat tcagcggatt atgaaggtat aataaacttt acagcaactg
    22321 atattgaagt tgaaggtaaa aaatacactt caaatgaatt tctgccaaga atagcaggtt
    22381 ttattgcagg aactccatca actcaatctg taacatatgc agaaatacca gaagttacta
    22441 atatacctaa gctaacaaga gctgaagcaa atacaaggat aaataatgga gagctaattt
    22501 taataaaaga aagtggagct atagtaatcg ctagaggagt tacatccttt acaacaatta
    22561 ctgattcaaa aggtgatata ttcaaaaaga ttaagttggt tgatacacta gatcaaattc
    22621 ataacgatat taaaaaaata attgtaaaaa attatatagg aaaaacccaa aacacttatg
    22681 ataataagtg tttacttatt gttgcaatac aattatatct tcaagaatta gaaaaagatg
    22741 gtttaattga tgaaggttca actgtagaaa ttaatttaga tgctcaaaga gcatggctaa
    22801 aaaaacaaaa tatagatata tctaatatgg aagaacaaca gataaaagaa tacaatactg
    22861 acactcttgt atttttaaaa gcaagaatta aattaataga tgcaatggaa gatgtatatt
    22921 tagacatctc tatgtagttt ggaggttaaa atttgaaagc taaaatagat gcaaaaagaa
    22981 taatatctgg tacttttgga gagtgttggc tagataataa gcaacttaca tcaacaaaag
    23041 ggatggaggc caaggttgat atagataaag aagaaataaa attatgtggt caaataatga
    23101 ccacatataa aatgattggt gcaactggaa aaggatctat aactctttat aagataagta
    23161 ccttatttgg cgagtatgtc gaaaaaatgt taaatgaagg taaagaattt aaagtaacta
    23221 taataagtaa attgcaagac ccagattcat atggttctga gagaattgca ctttatggat
    23281 gtatgtttga cgacttaact ttggctgatt gggaagctgc gaaagttgga gaaatagaag
    23341 ttccattcac atttgaaggc tttgaatact tagataaaat aaaagaaaaa taggaggatt
    23401 ttattatatg aacacagtag aaaaattatt aaatatggat gcaggtaaat taaaaatgcc
    23461 atctagcgca tttgaattat attgtaaaaa actagatgat actttagaaa tagagtgcaa
    23521 ggctattgat ccagaacgtt ttgacgaaat aagaatgaac tctatggacg ttaatagtgg
    23581 agatgtagaa aacattaaca tatatgaact taaagtaaat acaatattag aatcatgttc
    23641 tatatttagc aatatggaat tagttaaaaa atttagtgca attacaccta aagaattaat
    23701 taaaaagcta ttacttagtg gtgaaataga taaattatac gaggaggtta ataaagtaaa
    23761 tggaatagat gcagagggag ataaaaaaag acaaaaagaa agagctaaag atataaaaaa
    23821 ctaattaaaa cggatggaga agttaacttg atgtacttat tatttaagta taaaggtata
    23881 atgccatccg tttcttatca tatactaaga ggcgaaaaag aaattgttag tgtttttatg
    23941 gatcaagaat tagatgaaag aaaagaagaa ataaaatctg gtgttgggag gttgttttag
    24001 tatatgagtt ctaatacaaa ggtcctacaa gcaataataa aaatgagaga tgaagcaagt
    24061 tcaacattaa aaagagtagc agcaaatact agagctctac atgattcatc ttcccaagca
    24121 tcaagagcat taaataatgt tcaaagtaat ttaaaaagtg ttggaactag tgctttaaaa
    24181 gcaggaggtc ttatcactgc tggagttggt ggtgcatttg cagctgcaac taaagttgga
    24241 acagaatttg aaagcgctat gtcgcaagtt aaggctacat ttggaaattc cttaaaacca
    24301 ggagattttg aagctctgtc taaggctgct agagatgctg gaaaagctac aactaaaact
    24361 gctgctgatt ctgctaatgc acttcagtat atggggcttg ctggatggga tgttcaaaag
    24421 tcaatgcatg ccttaatgcc agtgcttaat ttatcagaag cagcctctat ggatcttggt
    24481 ctagcatcag atttagtaac tgatagcatg agttccatgt gcttagaagt tgataaggtc
    24541 agtaaaagtg gaaaagtact agctggtgca ggtttaagtg agtatttaga taaagttgca
    24601 aatgcatcta ctaaatcaaa tacaagtata caacagttga tggaagcgtt taatgtcgca
    24661 ggtggtacat ttagcaattt aaaagttcca ctttcagaag caactgctat catgggaata
    24721 cttgcaaata gaggagttaa gggtacagat gcagggcatg ctttaaactc tatcatggta
    24781 aatcttacaa caggtgcagg tcaagcaggt aaggcaatga aaaagttagg tgtaagtgca
    24841 tttgataatc aaggtaaatt taaaggtatg tcaaatgtgc ttaaagaggt tgcaaataaa
    24901 actaaaggta tgactgatga acagaaaaat tattacttat ctgctattgg tggaaagact
    24961 caattaaaaa cgttacaaaa gttacttgat ggtgtgggaa atgaatatga tagcttaaag
    25021 aaaaaaatag ataattcaaa aggtgcttta aaaacaatgt ctgatgtaat gaaggataat
    25081 actaagggtg gacttgcaac cttaaaatca gcccttgaag aaactggaat tactatttac
    25141 tatcaactaa agccagcaat agcatctgta gttgataatt taactaaaat gactgtttgg
    25201 tttaataatc taagtgaacc aataaaaaga gtcataacta atgtaatgct ttcagttgga
    25261 gcatttggag tacttttatt aactataggg gctgtgtctt taattgtttc aaaagctata
    25321 ggtatatttg gcttttttgt tccaataata ttaagagcaa aagttgcttt cagagctttt
    25381 gtaattggta tgaaaccatt aagtgcagta tttttagctg taatgggtcc tgtgggatgg
    25441 actataacag ctgtaattgc attatcagta gcatttacca ttgcttatgc taaatctgaa
    25501 gtatttagaa ataaggttaa ttcattagta aaatcattta cagaatttgc aaagcctata
    25561 gtaaattttt gtatgcctgc acttaaacag ctaggagaga aatttaaaga gttgttatca
    25621 gcatttggtc cactatttgc atctatttta aacctaggaa gtgtaataat gaaggtacta
    25681 ggacctgttt taatcggcgt aataggtttt catatagcaa aattagtttt gattttatct
    25741 actctggcaa ctgcttttaa agctgtagtt gatactataa ctaatgtatt aaatggtcta
    25801 gcatctattt taaatggagt atttgattta attataggta ttgtcacagg cgatagtaaa
    25861 aagatgatgg atggagttaa acaaatattt gatggtggaa ctaaaattat aaaatcaatt
    25921 tggaaaggtt tcatagattt tgtaactgct cctatacaag ctatagctga tatagctgac
    25981 agcttattta aagataaagc taaatggctt acagataagt ggagagattt aagaacattc
    26041 ttagaaaatc caattgaagc aactgccaaa atatctacta aaacaaatgt aaatatccca
    26101 ggaagtaaag cagcagatgc agctggtaaa tctacaccaa atacaggtgg attaaaacaa
    26161 acagtagcaa atctaaaagg ttcatttagt gattttaaaa cttttattaa gcctatgaca
    26221 gactctataa gtcaaggagt tgaatctgga aaaactaaat ttacagaact gcaaaataaa
    26281 atattaaatg atgttaaacc tgcactaaag gtcctagagg attctggagt aaattttaag
    26341 gataagttta aggagtcaat aacttcaata aaagataaat ttaatgaatt aaagaacata
    26401 ttagctggaa atattaaaac aaatatagat aatttagtaa aagcatttga acctttaaaa
    26461 cctcatttag ataatttgaa agattgcttt gataaggtta aaaaagcgat tactgatttt
    26521 tttactccag ccaaacaggt tggagataca gtaaacaaag taagccaacc aatggatagt
    26581 gttaaaaagt ctactgatgg aattaaactt tctttccaaa gtttaactca agcacttgct
    26641 cctgttaaag cttggtttca aggatttatg cctttcctat cacaaatagg acaacaactt
    26701 ttaccatcta taggaattgc aatagctggt gttgttggcg gaataatttt agcatttaca
    26761 tgggttttaa atacattgac cgcaataata acaacagtat ctggaatcat aaatggaatt
    26821 atgtctttta tatctggaat tattaatgtt attatggggc ttataacagg aaatgctgat
    26881 caaactatgg atggacttaa acaaatattt gatagtggag taggtgtaat aaaatcaatt
    26941 tggaatggcc ttgttgatct agttagtggc gtactagaac ctgtaataga tcttgctgat
    27001 tctcttttca aagataaagt taaaggggta aaagaagctt ggaataaact taaagatttc
    27061 tttaaacatc caattaaggg aacaatagaa atatttaaaa aaggtcctag tttacaaatt
    27121 ggaaaaggtg cagatgctgc tggcaaaagt ccaagaaaag catttggtat aaatagagtt
    27181 cctagaaatg attatttaat aagagcacac gaaggcgaga aacttttaac caaacaagaa
    27241 gctaatcaat acaaaaggaa tagttcaaat ggaagtttat ttcttgataa attggctgat
    27301 acaataattg taagagaaga ggctgacata gataaaattg taagaaaaat aaacaaaaaa
    27361 atatctcttg caaaaattgg aggtgttttg taaattatcg tgctataatt gtagtatata
    27421 atttacaggg ggttatgtta tgaataaaaa aatactagta ttaacaatat caattatact
    27481 atctttaagt ttagttggat gcagcacaaa taattcaaaa aatagagata gtaaatctaa
    27541 gcaaaacaca gaggaagtta aaaatcataa aataggcgaa gttgttgatt tagatggagc
    27601 aaaagtatct gttaaaaata ttaggaaatt agaaacaaat agtaatgatt atgctgttga
    27661 aattgaaata actaatacag gtgataatga tttacaaagt gattatctag aaaactatat
    27721 attaaaatca agtgacaata ctactggaga agcaataaat aaccaagaat taaatggaga
    27781 gttaatgttt gatacaatac gcccaggaga tactttgaaa ggtgaaatag cattcgaact
    27841 tacaaaagat gcaacaccaa aaacacttga aattgcttat gaaaatgtag gcaaatatac
    27901 agtatttgat ttatagtaat tatcttaaaa gacgcttatt tacttaagtg tcttttattt
    27961 taggaggagt ttaatggaaa tatggttaag acaagcaaat aatacattta ggtttcctgt
    28021 tattccaccc tcttttgaga taaatggaag tgcgacaata aatacatcta atattttaag
    28081 tgttggagat atcgcagtat ttggaggatt agggcttaaa actatagaac tatcctcttt
    28141 tttccctaat caagaatata gtttctgtaa ttataatgga tttccaaaac catatgattg
    28201 tgtaaattta atagaaagtt ggatgaaaga aggatatata ttaagattta tcattacgga
    28261 aactaatata aactttgaat gtataattgc agattttaat tatagagaac aagattattc
    28321 aagagatatt tattttacct taagtctaaa agaatacaga aggatacaaa tatccaaagt
    28381 aaacattaat aatgatgaaa aattatcttc tgaaaagaat gttcctctaa caaaaggatt
    28441 tgatactaag caaaaaacgc ataaggttgt tgaaggagat actcttttta agatagctaa
    28501 aaaatattat agcaatggag atttatggga gaagatttat aaagcaaatg aagatgagat
    28561 taaagatcca tctgtaataa aaaatggttg ggtattaata attccttaaa aatggtggtg
    28621 atttataatt aataaaatca aaatacaagt tcatataaaa aacggaaata tatataatgt
    28681 aactgattta gtggataaat gcacttggtc tggtgattat agatcaccat caagaacttt
    28741 ggaattttct ataattcaat ctgcaagtga tattaatttt aggcagatag atattccagt
    28801 agcaagtaca gtttgtttct atgtagatga taaagaactt tttagaggaa tgataataga
    28861 tagatctaaa gactctagca ataataatat agattttaca gctaaagata tgggtttttt
    28921 acttttacaa agcgaagtgt catacaattt taaagataaa ttagtcgaag atattgcaaa
    28981 acaggttttt gtagataata aattcccact aggtaattta cctaaaacta acgttaaata
    29041 tacaaaaatg tttattggtg taactggcta cgatactata atgagcgttt atacagaagc
    29101 aagcaagaca actaaaaaaa agtatatgat agagtctaat cttgataaat ttaatgttat
    29161 tgagaagggt gtagttactt taaatataac atttgaagaa ggctttaatc ttatcaatac
    29221 aaatttttct gaaagcattg agaatgtaaa aaataaagta ctagttgttg atcagtatgg
    29281 taataaaatt agtgagaaaa taaatgactc tatatttaaa gacgttgggg taattatgca
    29341 aaaggtaata caacaacaag aaaacagtac tatagacata gatggagagt ttaaaggaat
    29401 cgaaaaaact tgctctttaa aaggatatgg tgatataact tgtgtaactg gtagaggtgt
    29461 aaaagtaaaa gactcatata caaaattaat aggtcttttt tatatagata cagataagca
    29521 tacatggcaa aatggagatt atcaaattga actagagctt aattttgaga atataatgga
    29581 tgaaaaatct gctggccaag atgagcaaaa agaagatggt tcagagtata ctggaggaac
    29641 agaataccct gctgaattta cggcttattg tcctagaaaa gaagagggtg gaaatacaga
    29701 ttgtagaggt aaaaaactag atccttctaa aaagacatgt gccgctccta tggttggagc
    29761 atatgaaaaa tcttactata caaaagattt cttaaacaaa catcctctta tgagatatgg
    29821 agatgaaata caattagtta caggtgtttc aagccgagat ggaacgtata aagtaaatga
    29881 taatgggcct gcaataatta tagaaaagga tggaacatat catatagata ttttatttgg
    29941 aaatgttgaa gaagctaata actttggaag aagaaaagga aagattataa ttggtggtta
    30001 ttctggaaac atgacagaaa aagcaaaaac agtaatttca gaagcaagaa aacatcttgg
    30061 aaagccatac aaatggggtg ggaatggacc aagtagcttt gattgttctg gactaatggt
    30121 atattgcttt aagaaggtta atgttaattt accaagaacc tcaagtcagc aatctaaggt
    30181 aggtaagaaa gtagagaaaa ataatttaca ggcaggagat ttggtatttt tccataatcc
    30241 agtcagccat gttggactat atgtaggtaa tggagaattt ttacatgcgc cacaaacagg
    30301 agatgtagtt aaaataagta aattaagtaa tagaaaagat tttaatacag ctagaagagt
    30361 tttgtaaaag aggtgatatc gtggcagatc caatcaatga atttatagga ataattaggc
    30421 aagaaggtaa atttcataat gaaccttctt tttttattgg aaaaattaaa agtaaattac
    30481 cagatttaaa aatagagata aataacatca tattagaaaa agaagatatt ttgatagata
    30541 gttggatgct tgatagacag atagagttat ttgatacaga aacaagtcaa gaacataaac
    30601 atgaaataaa aaatcccttt atagatactt ttgaatctgg aaacacagta ataatgttta
    30661 aaataggtga aaaatttgct gttgtaagta aattggtgag tctagatgaa taatactata
    30721 ttccctttca tgggtgttcc agaagattat atttctccag acaatgaaga attacctatt
    30781 tttaaagaat ttgcttggga ttttaataaa gatgaaaaga taattgaaaa tggagacttt
    30841 aaaatagttg aaagaaatga agcaattaaa gtatggattt acaaaacaat aaaaacagat
    30901 aagtatgttc atttaatata tgattggagt tatggaacag atataaaaaa tctaataggg
    30961 caaaagtata ctaaaggtct tactgaaagt gaggcaaaaa ggtatataca agaagctcta
    31021 ttaactaatc catatatatt agaagtcaat ataacaaatg cagaatttga agatgataat
    31081 ttatctataa gtttaaatgt aaaaacaatt tatggagagg aggagattac ttttgtataa
    31141 tggccaatca ttcagtacct taaataatag aactttaaat aacataaatc tccctcttta
    31201 taaaggccaa ggatcttctc tttataatat agtttctcca ataaattcag aacttgcaca
    31261 attatatata gaactttctt atatacataa gagagttttt attcaagata attttgacga
    31321 ttttcttgat agaagagtca atgaatttgg cgtatataga aaattaggta cagaggcaat
    31381 aggtgaggta acatttgagg gcaaaattgg aactcaaata ccaaatggaa caataatatc
    31441 tcatagtgat ttactatttg taattattaa agatattaca atagatgaaa atagtaaatt
    31501 aaatgtaagc cctatacagg ctttagagat tggcattaag tataatttat cagctaacac
    31561 agaatttaaa cttatagaag aaataactgg agtaactaaa atttataatg aacttgacct
    31621 aaagggtgga acagaaatag aaactgatga agaattaaaa gaaaggttct ataaaataca
    31681 aaaaaatcaa gcgacaagtg gaaataaggc gcattaccaa tcttgggctt tagaggttga
    31741 aggtgtttat aatgcaaaag taattcctcg ttgggatggt ccaggtacaa ttaaaattct
    31801 aatttatggt caaaacaatc aatcagttga caatgaagta ttgcaaagat gtactgaaca
    31861 tatagaagaa gaaaaaccta ttggacctac tgttacaatt gttacaccaa gtacttttga
    31921 tattaatatt agtgcaactt taacacttga aaatggatat gatattgaat ctataaaagt
    31981 tgtgttttta gatattataa attcttattt gatagaaaat tcaagagaga ttgtttatat
    32041 aaaagtaatg agtttgttag caagcataga aggtattcat gatataaaaa atctccttgt
    32101 aaatggtgat gttaaaaaca taatagttga tgaagaaaaa gttcctgctg tttcaagtgc
    32161 tatatttgat atcgaggtga gttaaattga agttaattga ttacctgcca agttttgcaa
    32221 acaatgaaat tgacatacaa atccaagaag cacttgaaaa tgaactttta acgctaattg
    32281 atgaaaaaga tgatttatta gaacagtttt ttatagatac agcaacttgg ggacttgatg
    32341 attgggagaa ccttttaagc ataaaagtaa attataagtt agacttcgat actagaagaa
    32401 gcaatataaa agcaaagatg agaggtaagg gtacaacaac aatagaggtt ataaaagcta
    32461 tctcggaagc ttacacaaag accaatgttg atgtagaagt atttagtaat ctatttagtt
    32521 ttacacttag ttttataaca aataattgta gttataacac tattttagaa ttagataaga
    32581 aaatagaaga aataaaacct gcacaccttg aacacaaatt cgagaggata ttatttaata
    32641 aaaacgagct ttatacaggt gcagcaatta gtacaggaga aacagttaca atatatcctt
    32701 atgtacctag aaatttagaa agttttggag aaatagctat ttgtagtgga aatgatagag
    32761 cattagaaaa agtaacattg tatcctaaaa aatagaaatg aggtgataaa attgacagaa
    32821 caacaatatt ttactctagt aactgacatt ggtaaggcag caatagcaaa tgcaagtatt
    32881 acaggtgaaa aagtagattt tgcaaagata aaagttggag atggaggagg gagttcttat
    32941 actccaaatg agagtcagac agcactaaaa aatgtggttt gggaaagtac acttgaacat
    33001 gcacaaggag ataaagataa tcctaactgg gtagtaatac aaaaattcat acctggtgat
    33061 gttggaggat ttgaaataag agaagttggt ctatttgatt ctaaagacca attattagcg
    33121 atttctagtt acccaacaac atataaacct actgcggatt cagggactgt aaaagaacta
    33181 ttaataaaag taatattagt tgtatctaat gtagctaata ttaatttgaa agtagaccca
    33241 accgtaattt tagcaacttt aaaagatata caagaattag acactaaaat agatacaact
    33301 aaaacagaat taacaagcaa catagaaact gctaaaacag agttgaacac taaaatagga
    33361 gatacaacac aacttactac aacagataaa acaaatatcg ttagtgcatt aaatgaggta
    33421 aaaactagtg tagatagtat agaaacaaca gcagataaaa caagtataaa agatacagat
    33481 aatttatttg agagcgacaa cgtagaggga gcattaaaag aagtgatgca agaagtaaaa
    33541 ggtaatagaa gcagtattat atcaacaata aatagtaatt taataccaat gtagaaagtg
    33601 aggtgataaa gtgaagatat ggaaatacaa tagaactgtg aagaaaagaa gaggtgtcta
    33661 tacagaatta gatatacttg cacaaaggta tgaatttgaa gcagatggga aactttatac
    33721 agcttatttt catttatata ctaataagaa tatagatata ttcagagata aagatttata
    33781 taaagaatct tatatgatga gctatagagt tggaaatgta acaactgata ttgatgatgt
    33841 agtacagcaa acaactacag taagatttat acctgaccct gttattgata ttgtgattga
    33901 taacattagc agaggatgtg aaataaacta cagaattata gataatgaac caagtgtaaa
    33961 attcattgtt acagaaaagt taaatggcac aataatagaa acaaagacaa attctagtga
    34021 taataattat aaacttaaat taactgatga acaaatagta ccattagcat tcaattccca
    34081 aaattcaata gtaatagaaa taagtacaga agatggtgga ttagtaacaa gtaaaacagt
    34141 aacatttaca agaacaaata acaagccaac aattttagtt aattcatata gttctaattc
    34201 tgcaaaattt acagcatctg atttagataa taatttaagt aaaattgagt ggtatttaga
    34261 tgatgtatta aaagaaacaa taacaacaga tttaacagca gaaaaaacca tcaactatga
    34321 atttacagac aatgcaatac acacattaaa aatagttgct acagatgcag aaaatgcaac
    34381 agttgagaag gttttaagta taagtaaaga gataatgcca cttcaatctg atgctagttt
    34441 aagtgatata tcaactaagc tgatagagat tggagaaggg tttagaaatg gtaaaacaag
    34501 tattataaac actttagcac ttaaaaatat agaagcaagt ttaaataata cacttgtgga
    34561 gttatcagag aaaataaaaa caagttttga tagttcagac gctagtgtgc aggatttgca
    34621 aaatagaata acagaattga ataatcagct tagtcgaaga aaaaaatggg ctacaggtag
    34681 atacacattt actagagaag aagatactat caatttcaga atggttgatg gagatagtct
    34741 ttccgcagta aaaaaactaa ttatacccat taatttagat tttgttccaa gtgttattat
    34801 aatagacaaa atgtttttag gtagttacgt acctgcacaa gatgtgcaat ttaattgtgt
    34861 ttcaaactta gccgatttta cacttggacg tcgtttctat aataaacaat ggatttgcac
    34921 ttcttctata aaactagatt cagttacaca aaatagtttt actctttctt tacaaaaagt
    34981 tgttccattt gataaagtta gtagtacgat tttttctatt attaatactg gttttgcttg
    35041 gagagcatat gaataaaaat ataaattaga ggtgattaaa tgaatagaaa taatagaata
    35101 atttacgacc aaacaggcaa gatatggctt caaactggtg aagcaatagg agatatacaa
    35161 gagttgtcag aaataactga attaaatttc atagatgttg aatttggtag tatagactat
    35221 agtaaacaat atatagagtc tataaatcca gttacaaaag aattaaatat aaaaaatata
    35281 gaagttgttt tgactgatga acaaaagaga ttagaagtat tagaaaaaga actaaatatg
    35341 ttaaaagaag aaaataaaaa tagagattct gagatagtaa atacagcatt tgaagtagga
    35401 aatatcaaat taaacaataa tttataggag gaaattatat gtataactta ttaaagttaa
    35461 tgatagaaca aaaaaactat agcactaagg aggatttaca acataagatg gatgtattct
    35521 atgcagtaaa taggattaca gaagaacaat atttagagtt aacaggttta ttaaataaag
    35581 aagaaacacc agtagaacca acaatatagg ttctttttta ttgaaagaag gtgactaaat
    35641 gacttttaaa gaattagtta gtaaagttag aaatcttgta ttagaagcaa agaatgtaac
    35701 tatagaagat acagagaata aattcactag tgaaaatgtc gagggagcat tgaaagagtg
    35761 tatagataga gcagatttgg cttttcaaag tgccgatagt ggaaaagagt taattgcaac
    35821 tgctatcggc tctcctgcta catccgaaca aacatttcaa gagtatgcta attatattgc
    35881 aggatttaaa aatactataa gtcagttaaa ttctaaatat aaatatacta gtggaacttt
    35941 tacctattat actggtgcat atcctaatgg gctaaatata ggttttagac cagatattat
    36001 ttcctatact gccattaatg ctgtagagga aaaatattat tgtgtataca cacctttatt
    36061 ctatcttttt tataattata caactggaga aaaatatctt gatgttaatc ataatcgtta
    36121 tggaataact tttactgaca atggatggat acctagaaat ccagttaaca atggtgtgta
    36181 tagttatata gcagctaagt gccgttagtt gtcaaagact tagataaatt tctaagtctt
    36241 attttaatac aaaattagga ggaagtatga acgtaacaat agttttttta gcaacaaata
    36301 tatttataaa attagtaata ttagcaatag catttgatac attgctaggt tgtctaagag
    36361 caataaaaac acatcagttt aatagctctt ttggaataaa tggaggaata agaaaagttg
    36421 caatgatagc atgtatattt tttctagcag tagttgacat acttacaaag tttaactttt
    36481 tatttatgtt gccacaagat tgggttgatt tcttacgact aaatcatcta ggaatatctg
    36541 aatttttctc tattttattc attctatatg aaagtgtaag tatattaaaa aatatgtatt
    36601 tatgtggatt accagttcct aaacgattaa aagagaaaat aggtagttta ttagatacta
    36661 tgacagatga attaaatgtt aaaggaggaa aataaataat gaaaatatgt attacagtag
    36721 gacacagtat tttaaaaagt ggagcatgta cttctgctga tggagtagtt aacgagtatc
    36781 aatacaataa atctcttgca ccagtattag cagatatatt tagaaaagaa gggcataagg
    36841 tagatgtaat aatatgccca gaaaagcagt ttaaaactaa gagtgaggaa aagtcttata
    36901 aaatacctag agttaatgct ggaggatatg acttactcat agaactacat ttaaatgcaa
    36961 gtgataggca aggaaaaggc tcagaggttc tatattacag taataaaggt ctagagtatg
    37021 caactagaat atgtaagaaa ctaggtacag tatttaaaaa tagaggagct aaattagata
    37081 aaagtttata tattttaaac agttctaaac ccactgcaat actaattgaa agtttctttt
    37141 gtgataacaa ggaggattat gagaaggcta agaaacttgg atatgaaggt atggctaagt
    37201 taattgtaga aggtgtatta aataagagta ttgaaagtaa tgaggtgaag caaatgtata
    37261 aacacacaat agtgtacagt ggtgatgata gagtatcagc agatatttta ggattatatt
    37321 ataagagagc aaaagaaagt tatctagtta tagatataaa agactataaa ccacatagaa
    37381 cacaaaatct atatgtaatt ggtggagtaa cttgtaataa aatgaaagaa atgagtaaga
    37441 ctacaggaga aaaatttact caactatatg gtaatgatgt atggtcaaca atggataaag
    37501 ttatagaatt tgtaaaagaa aaattataaa ttaaaagaca acctttaaaa attaattgtt
    37561 aataaaacta ataaaatttt caactcagaa ggagcattat atgttaacat ttttagattt
    37621 gtttgctggc attggaggtt ttaggctagc aatggaaaga gcaggacaca aatgcgtagg
    37681 ccattgcgaa tacgataaat ttgcaaactt aagttacaaa gcaatgcata acccaaagga
    37741 ggatgaatgg tttgaaaaag acattagaga aattagagca gaaaatattc ccaaagcaga
    37801 tgtgtggtgt tttggattcc catgtcaaga cattagcatt gcagggaaca aacttggatt
    37861 caaaggaaat cgttcaagtt tatttttctc agttacaaaa cttattagag aacttaaaga
    37921 agaagataga cccaagtact tacttattga gaacgttaaa aatctactta gtgttaatag
    37981 aggatttgac ttcgccaaac ttctcattga gttggatgaa atcggctaca atgcagaatg
    38041 gcaaaatctc aattctaaaa acttcagagt tccccaaaac agagacagaa tatacattgt
    38101 tggacatctt agaagaagaa gtacaagaga agtatttcct atcacaggaa cgagtagaaa
    38161 aactactctt gagcaattaa ataatcctaa tcacagttca aacaggattt ataattcaaa
    38221 tggattatca aagtgtttat taagtaaagg tgggaacaat acaggtcttt atagagttgg
    38281 caatatcaat cctagtggaa atggtgcagg cggacaagtg tatagtagcg aaggcttttc
    38341 tcccacactt acaactggaa aaggtgaaag cccaaagata ttaattaaag agaatactaa
    38401 aaaaggatac atcaaagcat ctataggaga tgggataaga ctagatcata tagaaggtaa
    38461 aactggccgt ggaagagttc agcacaaaaa aagttgtact ttgactactg caagtaatgc
    38521 tggcgtaatt ggtaaaaaat ttagagtaag gaggttgact ccaaaagaat gttttaggtt
    38581 acaaggattt ccagacgatt attacgatag agcggcaagt gtctgttcag aaactcaact
    38641 atacaagcaa gcaggcaata gtgttactgt agatgttgtt gaagaaatag caaagaaatt
    38701 aaaatagaat attaatacaa tgcaaaatgt ggtaggtttt tccttccgca ttttgcattg
    38761 cattagcttt tttggaataa cttacctgta ctatcttaaa taaaaaaatg ctactatatt
    38821 ataaacattt acaaggagtg cataatgtta atacaaagct taaaacgtgg cgaattaatt
    38881 gtaatgaaaa tcttttagaa aagaaattca agaatatcaa agaaagaaat tattcgcatt
    38941 tgcaatgaaa aacacaattg gcggaaatct gcgacaaaaa ctctacttaa aagacttgtt
    39001 aaaaaaagag ttctacttaa gagaattaaa attttccaca tatattacat acctatagtt
    39061 acagaaaaag aatatttcaa atataaaata aatgatttag agccagatta tttagatgaa
    39121 tattttatac gattaatgtc cactgtacat aaatttaaaa atctagatga agaagattaa
    39181 atatctccct tcatctactc ttcacatatt ttaatagact ttcaagcaat aattgactta
    39241 acaaatcatg cttatcataa tgtttattct catctgcaaa tttattaaac atttcccaca
    39301 ctgtattatt tattctaact gtagttcttt ttatagattg atctggaaga tctatagata
    39361 tatcattttc tttaatgaca catatatgat aactatcatc tttagtttta aaccattcca
    39421 gcacattctt tatattatca atttcactag ataaatattt aatatcgtca tcaatcttac
    39481 tgctaatgct atctatatga tatttatcat cataactttt attatcttct acaaacttgt
    39541 ttccagttct tttatatcct ttcttattaa gccacttcct tatagtactt tcatttgccc
    39601 ctaattcatc tccaactttt ttaagacttt caaattcact taatttatta ttaatacaat
    39661 caacaacttc attaatattt ttttctttca aatcactcca agtcatataa atcctcctca
    39721 aaaaaataat agattacaaa tatcatatat ttgtaatcta ttatacatac tataaactaa
    39781 atcctttttt atttcatcat ttttttgtat ccttttgcat ttgcaaaaac atgtttatct
    39841 acatcactca taactattat gccaggtata ttctttttaa atgcttcacc taacaattta
    39901 gcgcctccac ccataacaac aaccgcagct gtactaactg gataatttaa tttcaattca
    39961 ttgtatattt ctttaaatag agaaatagat gcatctatat ttttctttat atcttgtttt
    40021 tcaccattta ggtaaaatcc tttatttatg tagtcttgta ttttttctct ttttatctca
    40081 actttaccta aaaatctttc gtccatctca agagatttct ttattgcatc atatatattt
    40141 attgttccca catttataga atatggatca gtcgctttat tcccttgtac aagcgcaata
    40201 tctgttgtct ttccaccaat atcaactaaa acaacatctc ttcctgcaat ttggtcaact
    40261 atgtcttttg cataatataa gtatcctgta acaccttctg gaaatgtttg aatagatctt
    40321 attgttatat ctcttttagt agtctcattt cctcttgtaa actcaacatg atattctttt
    40381 ccttgtaatt tttcaataag attttttctt aatccggcaa attgtttcac tggcagacct
    40441 agccctagat caacaatttc atcatctgta tttctacata tagcccctaa tagtagtggt
    40501 aagaagtttt ctttctcata tttaactata tttatttcct cgtcaccttc acctaggtaa
    40561 tatgttttat tgcctatttt aatgctatct gaatctgaac caaagttttt tacctctgta
    40621 actttacttt caaaaatatc atctgtactt gttttaactg cataatttcc aatatctata
    40681 cctaatttac tcatttatgt tatttccccc ttattattct aaacttccaa aatcaaaatt
    40741 gttagctgga tcttctattt cttttttctg ttcaactaca gcattatcct ttttttcttg
    40801 cattttttcc caaacaagct cttttaagta agctgtagct gataatttct catctaaaaa
    40861 cttttcaatt gctttttctt tatcattgtt tttaaatgtt agaactatcc tactcattca
    40921 accacttcct tttttataaa atattataaa atttattaat atttatatat attttactaa
    40981 attttacaca gtaattcaat agatttataa gatatttttt ataatatttt ataatttttt
    41041 acgtaatttt ataatttttt ataaaattta tcattttgta tataattaaa aacacacttt
    41101 ttataatttt gcataaaaaa agagtgtttc atatatttct aaacactctt ttctactttt
    41161 tataattatc taattgtaaa agaagcttca gctggactag agaataatat aatacctcct
    41221 gtacacacac cttttccttt aaatttagca atatatgaac ccttttttag ccctttaaaa
    41281 gttactacac cagttttagt agttccagct aatctaaagc ctacatactg agatgataca
    41341 attttgccag ccttatatac atctatattt ccttcaaatc ctaaaatatt tccaagcgta
    41401 gatgttactc tccaagataa ctgcccacca ctcattgcag ttaaattagc agtaccacct
    41461 ggaacaacaa cagatttagt cgaaggcgaa tctgagtttg gttggccaat taattcaact
    41521 gtttgtgaat ttccagtacc ttcataatca tttccatcag caaaagctgt aaatggaatc
    41581 attactgcgc aaagcgttag taataaagca attgtttttt tcatttgttt tccccctatt
    41641 tttttatatt attctcaatt acagtttcct ttctttacgt gccttaataa tcatattgta
    41701 attatgctca gcagtttcaa atgctcctac caaaggagtt ataattacac tttctattaa
    41761 gcacaattca tcatagttta aactagcttc tccagcatag aaattattta acttatctaa
    41821 atctattcta gttaaaacgg aaactgattc cattgagaat ttgtaaacat caataagttt
    41881 atataatcca cctatatagt tttttgctac ttcatggctc ttaaaaaatt catcttttcc
    41941 tgtatgtagg tttacttcca taattccctc cattgttctt atatttttaa ccttcaaata
    42001 atatttactt cttcttcaaa acagaatatt ctatcacggt agaatattct gtttttttca
    42061 ttctatcatg atagaataat attttcaatc attttttcta ttctatttta tttaaataga
    42121 atagaaaaaa agaacactta atatttttca agctaatata ttaagtgttc tttttatatt
    42181 tttacaagat tatataaaat tattctttaa tcaattaaag tgtttttcta tttcttaata
    42241 ataagaaatg atataaaatt gtatcatttt aaagattttg tgatataata aaagcaagaa
    42301 gaactacaat ctatttaggc gtagagtgga gttcataata aacgtagttt attttttgaa
    42361 tttaaatata aatttaaatt caacctgtaa gtcactcttt gcacgagagt ggctttttgc
    42421 ttttttgaat agttcactga ttaagtaaac taccacacta gctgttaaac tagctaaaac
    42481 attaaacaaa aagttatcca tgtaactcac ctcccctcga atcgttggga ggataatctt
    42541 ttgtacatga actccactct atagattgta tattacgctc ttcttgctaa atttaattat
    42601 accatatttt gtaatatcag ttaagagttt gcactacaat aatcagaaag cttgttccac
    42661 tcacaatcaa cccctattat tttaagaata tcattgactt ggctatttaa atctttaata
    42721 tctgatttag acattaaagg atctttcatt atttcattta gaaaaatact atcaatgatt
    42781 aaacatacat tttctgataa atattcacgt tttactgaga ttactgttcc aactgtactt
    42841 tcttttagtg tcttatatgc tacactcaat atgcatggtt ctaaattaat tccttctagt
    42901 actgtttcaa agtaatattt tattcttata ttttcatttc caataaatat ttcttcattg
    42961 ctgcttatct tctttaattt tctcattatg cctaacatct tagcattcac ttcatagttt
    43021 attccacatc tctttgatgc taaactcgac atcaagtctc tttctgaata aattacccat
    43081 tgaccattat aggaaaatga tgttttagcg atatccattt ctccttttcc agtaatatta
    43141 aatttaggct ctagtttatt tattaagtaa atctcataaa aatccatagt atatttatta
    43201 tctaattcta tatactcaaa tcttaaattc ttattacacc aattttcttt tttattactt
    43261 aaatgactag cgtgtctatc gcagatattc tctgttttcc caacgtataa acattcttcc
    43321 gtatctagat taatatattt ataaacataa tacatttttt attttctcca attattattt
    43381 atagtaatat ccttcttcat ttactagacc ttgatcaaat aaatttctcc taacagtttc
    43441 aagtctgaca ggatctttgc tataatcaac ttctctttct tcttttttaa catcttcatc
    43501 atcccaatac ttatccaatt cttctttaaa ctttaaatca tctttttgat gaattataac
    43561 ttcacttatt ttatttgaaa gtgttgattt aaaaaaatca taattctttg tatttataat
    43621 atcagtatta tctctttcaa atgctatagc aatagactta ttaaaagcat tcagataaac
    43681 atctttctta aaatcaaact ctttaaaatc ttctttaaat agtttaatcg tccctgttgt
    43741 aaaaatattt tcatttggta tataaaaact aaaagacact tcattgtcct gcttcttttt
    43801 aggtatataa tcttcaacca atatatcttt atcaaaatat tttctaggtt cataatctat
    43861 cacagaaaaa attatttcat ctatttttct attttttctt ttttcttctt ttattgaaac
    43921 aaccatgttg ccttttttat ttatttcatt cattgctctt tttagaacat tttgtttaaa
    43981 atatttatac tctggatata cattatcttt taatttcaaa taaaatctta attcatctaa
    44041 cttatatttt atctccactt ccttattttc tctgctccat agtctaaata aagtgtataa
    44101 cctctgagta tatgcacctc taaaattaaa taacactgat aaattaatag ctgtatatcc
    44161 atttttcttt ttctgcattt ttacaaaatc agttatatga ttgtatagaa cttcatgcat
    44221 cataattgta taaatttggt ctgtatgatc caattcatat gtattaatta aaccacttcc
    44281 aaaagttttt aacttgccag ttgtctcttc tatatagtca aattccaaaa cactttgttg
    44341 aaacatgttc aaaatttctt ttatattttt atgctcataa tcattattgt ttttcataaa
    44401 tacttttagt tcttcttttg atatagttgt aacataagat gaattttgct gtttctgagc
    44461 attaaaaagt attttgtaaa ataatttatt ttccacattt gtaaaatcat atttactttt
    44521 tattaaatta tttggttgca ttaaaatttc ttttttttcc aaaatctatc acctcatata
    44581 aattataata cctaatttat aaaaaggtca accatttttg aaagacgctc acctttgacc
    44641 cttaaaatga tgacctttga cccttaaaat gatgaccttt gacccttaaa atgatgacct
    44701 ttcgaccctt aaaatgatga cctttgaccc ttaaaatgat gacctttgac ccttaaaatg
    44761 atgacctttc atctctgaaa cctagatata ccaattatta tagcttgtct aaaaactatt
    44821 aaagacttta aaaactgttt taaaaactat taaagactag aaaagatgtt ctgaactatt
    44881 ctttttttat attaaaaaac tttagtattt atttttctag tatactttaa acacttacta
    44941 aaaataattt cttataaaag attggagatg aagaattttt tatgcttgaa aacagaaaac
    45001 aaattcagaa agaaatttac ttaccaatag caacatattg tttaaaagat aagcaatatt
    45061 atataaaaga atatggagca ctattaataa agagtgctga aaatagtgac atttttaata
    45121 cccataaaaa tagaattttg tataagaata aacttgatat tcaagaaata gcaaatgaac
    45181 tgaatgtaaa taaagcaaca atccaaagaa atataaaaaa actagagaag ttagatttta
    45241 aaattctaaa aatagaaaat accctaaatg gaattgtata ttgtttacca tcagaaaata
    45301 atattgattt aaacaaattt gcattgataa attatgaaat gcttaaaaaa atggtcaata
    45361 aatttaaatc aaatacaata aaagtatatt ttttacttaa aacaatcact acagaaacta
    45421 attttaaacc tgcaactaat agttttatag cagaaaatat aggactaagt tctaaaagta
    45481 aaaataattt agatatcata acttcttctg ttaaaacttt agaagaaaat aaatacattg
    45541 aaacaaaaaa agtaaatgta tatgaatatg acaaagaaaa acttagagaa gttccaaaaa
    45601 taagaaaagt ttatcggatt tgtaattttg atgagtggaa aaaggaaatt gataaaaata
    45661 aagtttatta aatatagctt aaaagcattt aaaggcttgt ctgacgtgtt ttaattacaa
    45721 acttgatgtt atttacctaa aaagatacaa atttgcttaa aaatggaaat agaaggtcaa
    45781 atatataaat agagaaccaa ataaaattat attggctctc tatttatctt cttcccaatc
    45841 ctcaataaac ttttctaaac tttttaattt attttcagac acttcttcat catcatgcag
    45901 agcacttata aaactttcaa aagaattttt gtgaaagaaa ctaaagaaac ttttagtctc
    45961 aacttttaaa tattcttctt tttctattaa tgctttataa tatgtaaatc tatctttctt
    46021 aacaacctct aaaaatcctc taccaactaa tctcctcaag aaagttaaca tagttgattt
    46081 tttccattcg tacttttcgc ctagtgtctt caaaatttca gtagatgcaa cttctttatc
    46141 ttcgctgtcc caaataaaca tcataaccaa caattcactt tctggaatct tttttatctt
    46201 cataaagtac accaccttct aaaagtatat ctaaaatact ataacaatag agttaactac
    46261 acaatactaa atgtctaaaa atgttttacc aatttttacc aattaatttt tcttt
//
LOCUS       DAYAOY010000023        46239 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYAOY010000023 DAYAOY010000000
VERSION     DAYAOY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.023"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(25..2154)
                     /locus_tag="ACGI6B_001371"
     CDS             complement(25..2154)
                     /locus_tag="ACGI6B_001371"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFG9750825.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            2378..2668
                     /locus_tag="ACGI6B_001372"
     CDS             2378..2668
                     /locus_tag="ACGI6B_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750826.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            2834..4225
                     /locus_tag="ACGI6B_001373"
     CDS             2834..4225
                     /locus_tag="ACGI6B_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFG9750827.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            4340..5809
                     /locus_tag="ACGI6B_001374"
     CDS             4340..5809
                     /locus_tag="ACGI6B_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFG9750828.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            5874..6467
                     /locus_tag="ACGI6B_001375"
     CDS             5874..6467
                     /locus_tag="ACGI6B_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9750829.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            6598..7995
                     /locus_tag="ACGI6B_001376"
     CDS             6598..7995
                     /locus_tag="ACGI6B_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9750830.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCV
                     LIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEP
                     EREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKL
                     LGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPD
                     SMYNNSKIKEILSEK"
     gene            8177..8548
                     /locus_tag="ACGI6B_001377"
     CDS             8177..8548
                     /locus_tag="ACGI6B_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFG9750831.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            8541..9230
                     /locus_tag="ACGI6B_001378"
     CDS             8541..9230
                     /locus_tag="ACGI6B_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFG9750832.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            9750..10616
                     /locus_tag="ACGI6B_001379"
     CDS             9750..10616
                     /locus_tag="ACGI6B_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9750833.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            10858..11667
                     /locus_tag="ACGI6B_001380"
     CDS             10858..11667
                     /locus_tag="ACGI6B_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFG9750834.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            11811..12875
                     /locus_tag="ACGI6B_001381"
     CDS             11811..12875
                     /locus_tag="ACGI6B_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFG9750835.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            12903..13622
                     /locus_tag="ACGI6B_001382"
     CDS             12903..13622
                     /locus_tag="ACGI6B_001382"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9750836.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            13646..14230
                     /locus_tag="ACGI6B_001383"
     CDS             13646..14230
                     /locus_tag="ACGI6B_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFG9750837.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            14232..15581
                     /gene="pabB"
                     /locus_tag="ACGI6B_001384"
     CDS             14232..15581
                     /gene="pabB"
                     /locus_tag="ACGI6B_001384"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFG9750838.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            15571..16314
                     /locus_tag="ACGI6B_001385"
     CDS             15571..16314
                     /locus_tag="ACGI6B_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFG9750839.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            16348..17049
                     /locus_tag="ACGI6B_001386"
     CDS             16348..17049
                     /locus_tag="ACGI6B_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750840.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            17075..17647
                     /gene="folE"
                     /locus_tag="ACGI6B_001387"
     CDS             17075..17647
                     /gene="folE"
                     /locus_tag="ACGI6B_001387"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFG9750841.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            17711..18502
                     /gene="folP"
                     /locus_tag="ACGI6B_001388"
     CDS             17711..18502
                     /gene="folP"
                     /locus_tag="ACGI6B_001388"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFG9750842.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            18513..18878
                     /gene="folB"
                     /locus_tag="ACGI6B_001389"
     CDS             18513..18878
                     /gene="folB"
                     /locus_tag="ACGI6B_001389"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFG9750843.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            18871..19377
                     /gene="folK"
                     /locus_tag="ACGI6B_001390"
     CDS             18871..19377
                     /gene="folK"
                     /locus_tag="ACGI6B_001390"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFG9750844.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            19358..20143
                     /gene="aroF"
                     /locus_tag="ACGI6B_001391"
     CDS             19358..20143
                     /gene="aroF"
                     /locus_tag="ACGI6B_001391"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFG9750845.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            20398..22188
                     /gene="dnaG"
                     /locus_tag="ACGI6B_001392"
     CDS             20398..22188
                     /gene="dnaG"
                     /locus_tag="ACGI6B_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFG9750846.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSSDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            22206..23372
                     /gene="rpoD"
                     /locus_tag="ACGI6B_001393"
     CDS             22206..23372
                     /gene="rpoD"
                     /locus_tag="ACGI6B_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFG9750847.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            23537..24226
                     /locus_tag="ACGI6B_001394"
     CDS             23537..24226
                     /locus_tag="ACGI6B_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFG9750848.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            24240..25337
                     /locus_tag="ACGI6B_001395"
     CDS             24240..25337
                     /locus_tag="ACGI6B_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFG9750849.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            25400..25972
                     /locus_tag="ACGI6B_001396"
     CDS             25400..25972
                     /locus_tag="ACGI6B_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9750850.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(26032..26499)
                     /locus_tag="ACGI6B_001397"
     CDS             complement(26032..26499)
                     /locus_tag="ACGI6B_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9750851.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(26885..27361)
                     /locus_tag="ACGI6B_001398"
     CDS             complement(26885..27361)
                     /locus_tag="ACGI6B_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9750852.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(27481..28572)
                     /locus_tag="ACGI6B_001399"
     CDS             complement(27481..28572)
                     /locus_tag="ACGI6B_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HFG9750853.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(28565..28954)
                     /locus_tag="ACGI6B_001400"
     CDS             complement(28565..28954)
                     /locus_tag="ACGI6B_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9750854.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            29146..29592
                     /locus_tag="ACGI6B_001401"
     CDS             29146..29592
                     /locus_tag="ACGI6B_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750855.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            29856..30581
                     /locus_tag="ACGI6B_001402"
     CDS             29856..30581
                     /locus_tag="ACGI6B_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750856.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            30559..31812
                     /locus_tag="ACGI6B_001403"
     CDS             30559..31812
                     /locus_tag="ACGI6B_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9750857.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            31892..32569
                     /locus_tag="ACGI6B_001404"
     CDS             31892..32569
                     /locus_tag="ACGI6B_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750858.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            32570..35131
                     /locus_tag="ACGI6B_001405"
     CDS             32570..35131
                     /locus_tag="ACGI6B_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9750859.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            35352..36626
                     /locus_tag="ACGI6B_001406"
     CDS             35352..36626
                     /locus_tag="ACGI6B_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HFG9750860.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(36787..38799)
                     /locus_tag="ACGI6B_001407"
                     /pseudo
     CDS             complement(36787..38799)
                     /locus_tag="ACGI6B_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
     gene            complement(38901..40742)
                     /gene="ltrA"
                     /locus_tag="ACGI6B_001408"
     CDS             complement(38901..40742)
                     /gene="ltrA"
                     /locus_tag="ACGI6B_001408"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFG9750861.1"
                     /translation="MYNTDTKSIIKQDLQPLKKDKLRYNEYYNLQNTFDNLYKQSLEG
                     SKFTKLFNLISSEENILLAYRNVKTNTGSKTAGTNRHNINYWKTKDTKIFVQYIQNRL
                     MNYKPQKVRRVEIPKPNGKIRPLGIPCIEDRIIQQCIKQVLEPICEAKFHPHSYGFRP
                     NRGTEHALAYVVKKINFEHMYTVIDIDIKSFFDNVKHSKLLKQLWSIGIHDKKLLSII
                     SSMLKAEIDKVGIPNKGVPQGGILSPLLSNIVLNELDWWISNQWQTHKTHYQYSTTSH
                     LYRALRKTNLKEMYIVRYADDFKIMCKNVSEANRIFIATQKWLKERLGLEISQEKSKI
                     TDVRYSNSEFLGFKIKAYKKRNKYIVYTKMTDKAKEQVSHNIKEQLIAIQHFPTTKNI
                     NMLNRIIAGSHNYYKIVANVYQDFKNIKYNLSFSLYNRLKNLRTSTGNLTHEYESKYS
                     KYNLKKQYILNEIMYPLTGIKTTPPFLFNQTVSNYTKEGRKIIHTRLGCIDNNILNYI
                     AHNPILNKSVEYNDNRLSKYSAQKGTCIISGYPLVENMEAHHITPVNKGGDDKFRNLI
                     LVIPEIHKLIHATNIEHIQYYIKELNLDNQEIKKLNKYRKKIGNEII"
     gene            complement(41181..41426)
                     /locus_tag="ACGI6B_001409"
     CDS             complement(41181..41426)
                     /locus_tag="ACGI6B_001409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750862.1"
                     /translation="MKRIKKVFRDLVENYKEEKFEKQEAIEMQEAIEAIRQEIGRHDP
                     TINTPKDSAGNVGDEPPFISNPLVANQPIKNPKYKGN"
     gene            complement(42140..43171)
                     /locus_tag="ACGI6B_001410"
                     /pseudo
     CDS             complement(42140..43171)
                     /locus_tag="ACGI6B_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
     gene            43377..43676
                     /locus_tag="ACGI6B_001411"
     CDS             43377..43676
                     /locus_tag="ACGI6B_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFG9750863.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(43915..46143)
                     /locus_tag="ACGI6B_001412"
     CDS             complement(43915..46143)
                     /locus_tag="ACGI6B_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFG9750864.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        17949 a         5275 c         7613 g        15402 t
ORIGIN      
        1 actataaaat tttactacat tctattagaa ttgcccataa ataccttcaa gttcttcttt
       61 taatacttca gggtcattta taccaataga catggttcta gccagtatat ttgcttggtc
      121 ataagttgat gtccatgaga atacaccacc tagacaattt ctaagaacat attgagtttt
      181 taagaatatt gataaagcat catcatatga cattgcaaaa tctccatcct taactaaata
      241 cggaacttga gcagtattat caaatctata ttcatatcca tttttattta tataatccct
      301 tctaagctca tcataagttc ttgttatagt agcacctaat cttccataaa atggtatacc
      361 aagaaggatt ttttcagaag gcattccagc attctcaaga tttcttacca ttgcatcaac
      421 actatatcct ggcaaagata agtctgaatc aaaaagattt gcttgatgtt ttctaccatt
      481 tgggcctgtt tcacctgctg taaaatcata actcataaga ttaaaataat ctattatagg
      541 agctatttta tctatttcag cacttgaatt tatatatcct ctatctcctg ttccagctac
      601 actaagccat ttatcatctc ctataacatc tcttatggca gttagtaaga gtgtaaagtt
      661 ttctctatct tgaggtcttg atgttattcc agatgcacta cttccaggat attcccagtc
      721 tatatctatt ccatctaaag catattcatt tatcatctga ttgacctgtc ttgcaaaatt
      781 atatctagat gtaggtgtta atgctgcatc agagaatcct tcagctcccc aacctccaat
      841 agctacaatt acttttaagc taggtttttc acctttaaga gcaagtaatt gatttaaaaa
      901 tctaggataa ggaataaaca aatttccaga ctggtctatc tcagcaaaag caaatataac
      961 tgcatctaat agttgagcat ctacattagt tggatttcca agaacatatt ctgctacaat
     1021 tggatttata tctggacagt ttgcattttc aactattgga ttgtatgggt ctgttatttg
     1081 tggtctaact aattttttag aatcttttaa agttttcata gctatttttt tagtattttt
     1141 ttcgctagta taatcgtagt ttttataatt atgtttatag aaatcttcat aactactatc
     1201 ttctttgtta tagctagaat ttattttgtc acaagattca tctttatcat agttaaaatt
     1261 catttcatca caagacttat ctttaaactt atcttgtttg gagttgtgaa ctttgttagt
     1321 atttttagta tgttttttct catgattgca atcatcttta atatcagtct tgtgttcttg
     1381 tttacagtca agagctttgt tcaggtagtc atgatgttca tgttcacagt tacattcatt
     1441 ttcaatgttt ttagtatgtt cttttttcca gctacaagta ttatcaattt tcttgctcac
     1501 ttcttcatca ttataattat ctggtacaaa acataagtac cagtccatac atgaatattt
     1561 cttattacaa ctgttaactc tattttttaa ttccttatag tttttaggat aaggtaaaat
     1621 cactggcatt ccaggaaacc agtttgcagg agtaactata ttatctctat cactagtctg
     1681 aagtgcatct actattctaa gtatttctgg aatatttctt cctgtagtta gtggataata
     1741 aagaatcgtt cttagaattt gattattatc tataataaat acagagcgaa cagttgatgt
     1801 atcactcatt ggttttgata tcatgccgta taacttggca attctcatat ctctatcttc
     1861 tataatagga aatggaattt ctacacctgt aagtaaagaa atattataca tccaagctaa
     1921 atgtgaactg ttactatcaa cacttagacc aattagttct gtatttcttt ttttaaattc
     1981 gtcataatat ttagcaaaac ataaaaattc tgtagtacaa actggtgtaa aatcaccagg
     2041 atgtgaaaat aaaacaatcc aattaccctt atagtcagag agtctaatag gaccatttgt
     2101 tgtattggct ttaaaatcag gagcctttga ccctaaactt ggcaaatttg gcatgtaaat
     2161 cacccctcaa taaattatca tttattttat tctattaatt aatacatatt agtgttacta
     2221 ttttattaaa agttaagtaa tccttatgtc aaaaaaagat atttattaaa aagatttaat
     2281 attttcctaa tataaatgac aatagtaatt gctatttttt tactaatttg gtatagtaat
     2341 ggataagggg tagtgtcaat tttagtgatt atacgaggtg agtggattga aaaaaattct
     2401 aagaatttta gttatctcca tgatgatatg ttttgcaatg acttttgcat tagtgttaat
     2461 aaattcagtt atgggagtac atataggatt ggattattac tttttgatgg gaagtgtgct
     2521 tacagtttgt ttaacaattg gaattgtttt atgcaatgct agaaaaatat ttgatagcaa
     2581 agctatacac aagacaagaa cgaaaactgt cagtaataat aaaaaacaaa agagtcattc
     2641 tagggatata aaaagaaaga tatcataatt tatgtaaagg aaatatttat gtataaaata
     2701 atgattgata ggtaaaataa aaacacgatt taacttgtgt ttttattttt tttcaagaat
     2761 tatgatataa taaccaataa gaaatttagt ttaccataaa atataaagat tattaaatgg
     2821 agagggaact ataatgaatt ttagagattt atttgagaag gctgtagatt ttattgatga
     2881 aaaaaggaaa tctatagttg cgatttcatt atcaacatta ggtgtaattg ctttgataat
     2941 agtatttttc ttaagtagta acgagtttag tgtaggaaat gaagctaatg agttgttaaa
     3001 gattatagag aaaagacagt attctatagc tgttgattac tacacaagta tagaaaaaaa
     3061 gttttctgat tcaaaaatgg aaaggtttaa taagtctgtt tctaaaaaaa taaataaatt
     3121 gctattaaat agtggtgata aatatttaga tggagacata agtcaagaaa gttttattgg
     3181 attaataaat accgtgaaag aattaaacaa aataagtata gatgtggatg acttattagc
     3241 tcaagctgat agagttagag aaatgtataa agaagaaaat actacttatg atattgccat
     3301 taactacata agtactgttt caatattaaa tggtatgggt agtaacctag atgtatacaa
     3361 acagaatata gaaacaatca aagagtctag agatgtttat gactcagcag taaaagacca
     3421 aaaagcatat aagtattatg aagctattga aaagtataat aaagtattaa aagaagatga
     3481 aaagtattac agtatggctc aaaatgggaa agaacaatgt attgaagaaa tgtatgatta
     3541 ctatataagt agagctgaag aggcaaatac ttctggagat tatgaaagag cattacaata
     3601 tattgagtat ttaaaagacg attattcaga tgatgaaaag gtacagagtt tagaaaaaaa
     3661 atataaaaag aatttatcat tgtatacttt aacatctgat gacattataa atgtaatagc
     3721 taaaaaaagt ggcaaagaca aggcaaatct atctataaat tcacttccac aaatgataaa
     3781 aaataataaa tactattatg ctgaagtata tgagtatgat aaattaataa atgaagtttt
     3841 ggtaagtgca aaaactaaag atttatattc atataaagat ggtaaaaaag attacaaagt
     3901 agattatggt gatggatact ttagaatatt agaagatgga agttatcaat ttggtattac
     3961 aaaagataaa gctaagtttg tgttaactaa tactcttgat gaaaaagaaa ataagtataa
     4021 aaagattgag atattagata tagagaaagc agatagatat gtaaaaagta aaaagagttt
     4081 agaagaattg tttggaaaat ataaaaatat ttattattat gctgtagtaa ataaaggctt
     4141 atttagaggc aaagaagttt atgctattaa tatttacaat gaaaaaatat atgccatatc
     4201 agaaaatgga ctaaatgaat attaattaat aatattaaat taatatataa ttaaaaagtt
     4261 tttggggttt taaggcgcat tatgtagtaa tataataata gggatatata aataatttta
     4321 ttgtaaggag aagtatggga tgagtaatgt gaacaaaaag ctagttatag ctattggagc
     4381 tgtagcatta gtagtaataa tttttatagg cataatgtgc atgcaattta aaggggagaa
     4441 aatagcaaaa aacacatatg ttaatggagt aaatattgga aaattaacta aatcacaagc
     4501 aaaacaggaa ttagctaaaa aatacaaatt agagaatgtt gattttaatt acaatgataa
     4561 aagctggaaa gtaaagagca aagatttaaa tttatctact gacctggata aaacagtaga
     4621 aaacgcatat aatttaaata gaaagagtgc tttctttggc aatctatcaa aaactatttc
     4681 agcaaatttt ggcaaaaaaa gcaacttagt agttgcgata aattatgata aaaataaatt
     4741 aaaagcagaa atggaaaaga tagctaaaga aatagatgta gatgtaaaag atgctacact
     4801 tgatataagt ggtgaaaaag tcaaggttat accagattct gatggtctta aaatggatat
     4861 ttctaagagt atggagaatt ttgataatca aactaaaaaa ggtaactata aaaatgagtt
     4921 agtagtaaaa gctactccag caaaagtaaa aaaagagcaa cttgctaata tagatacaaa
     4981 tttaggtacg tattcaacaa catttaaaac ttctcaaata aatagaagta taaacataaa
     5041 attagcaaca gataatataa gtaatgtatt acttatgcca ggagaaactt tctcatttaa
     5101 taaacataca gggaaaagaa gtaaggaaaa tggatataaa agtgcacctg taataatgga
     5161 aggtgagatg gaagaagatt atggtggagg agtttgtcaa gtttcatcta cgttatacaa
     5221 ttctgtgcta tacgcaggtt tagaaattgt taatgtaaaa aatcatacta taccatcaag
     5281 ttatgttcca aaaggaagag atgcaactgt tgcagatagt ggaatagatt tcttatttaa
     5341 aaataatcta aagcatccag tttacataaa aaattacgtt tcaggaaatc aaattgtttg
     5401 caatatatat ggaagtgcag aagataaaca aaatataaca atatcaacaa aattagatgg
     5461 cgtttcacaa actacaatga aaagagttaa tgaccctaca atgcctaaag gtaaagaaaa
     5521 agtagacaaa agtggaagaa atgcatattc tgtatcaaca tatagaacat ttaatgatgc
     5581 aaatggtaaa aaaattaaaa ctgaaaaaat agctaattca tattatccta agaaggaagg
     5641 tattatatta gttgggacta tggaaccaaa accagaggaa aaaccaaata cagacgaaaa
     5701 taagaataat caaaatacaa acaatcaaaa cccaaataat caacaaaaac cagaaacacc
     5761 accaactgat aataagccta atgagacaca accacaaccg caagcttaaa gttctcaaga
     5821 ataggattta tgtagagtaa taaaaaatat aaatctaaat aggagggatt actttgaatt
     5881 taaataatat aacgaattac gttcaaagta tttgcgagaa tatttctaat gtcttggatg
     5941 tagatgttac tttagttaca aaagatttaa ctaggatagc aggtacagga atattcagag
     6001 gtaaaatagg agagacaata tcagagaagt cagtgtactc tcttgtttta aaagatggga
     6061 aatcctatgt tataaataaa gaaatagaag agaactgtaa taaatgtgca aatagagaaa
     6121 attgtaaaga gttagctgat atatgtactc ccgttaaaat gggagagcat atacttggaa
     6181 tcttgggtat agctgcgttt aatgaagaac aaaaagaaaa gatactttca aaagataagg
     6241 agttaactgc ttttgtaagg agtatgtctg atttaatatc ttttaaacta gatgaacttt
     6301 ataaacaaga agttaagact atagatgacc ttgagaggga agctatagaa aatgccataa
     6361 agaaatatgg aagtaataca gaaggtatga aaaaagtagc tgaggcatta aatataggta
     6421 tagctacact ctatagaaaa gtaaaaaaat tcaacattaa aagttaatag tattgaaaaa
     6481 gaggtaacct ttttatgaaa agaagaggat tacctttttt attatgagaa tggtggaaaa
     6541 atatgtatag ttttgacttg acatattaaa tatgttatct taaatatata ttttagagtg
     6601 gaggtgagta ttattttagt taaagatata aaacaagaca tagaaagcat gcctagaaaa
     6661 attttagata tatctttgat agaagataat aaaagtgatt tgactttttt taatatagaa
     6721 gaagaagacg gtgatgaaac attaaaagca ttaaaacaga gttatgtgac tacaaattat
     6781 aaagaagaaa ctctattaaa cttgcaagga gctttaaatt caaagtcaga tacaacgtct
     6841 aaggagttaa aattagaatc atataaaaga tataacaatg cattggattt agcatataaa
     6901 aattatataa ctagtgccat taatatggtt aataaagctc tggagataaa tcctaaagac
     6961 attgatatac taaatcttag aggtctttta aaattattaa aatgtgattt tgctaaatca
     7021 tttgagagtt tttatactgc tttatgttat gaaaataatc atatatctag aaaatatgta
     7081 aatctaattt catctgaaga ttttaaaact tttttaggaa gatacaatca ttctataaga
     7141 tttataaatg aagaattaaa ttatgagtca atacatattt tagagaacat aattgaagaa
     7201 gaacctgaac taatagagcc atatgtaatt ttatctttgt tatatgataa actaggtaat
     7261 gtaaaaaaga gagaaggata tttagacaaa cttaaagagc tagataaaga taatcctgta
     7321 tttgaaaaaa atgaagaaga aaaagaagat acaagtaaaa aggaagaaat aaaaaaagta
     7381 aaaagaaaga aaagtatttt gccatatatt atagttggct gtgttttaat aggtatgggg
     7441 atatatttga ttcaaagcaa aaagagaata gaaaacttga ataaccaaat aagtagtaaa
     7501 gaggaaaagt taagtgaaac tgataaaaag ttaggagaaa cgagtaaaaa attagaaaaa
     7561 acgaataagg aattaaatga ggttaaaaat aaagaaccag aaagagagat aactgtagct
     7621 gatgaggaag atttatatta tcaagctcta aatttaaaga aaaacaaaga atatgaaaaa
     7681 gccatagata attttaaaag tgtagtttct tctggtaaaa ctaaaaaata tatttctgag
     7741 tcaatatatc aattagcaat aacaaataaa ttattaggaa ataaggatga agctataaaa
     7801 tattacaaaa aatatataaa tacttatact aaaaatgacc agtattatga tgattcatat
     7861 tacgagttgg gtatgcttta ttatgataat ggcgatttaa aaaatgcaca acagactttt
     7921 tatagtttga gaagtgaagt tccagacagt atgtataaca attcaaaaat aaaagaaatt
     7981 ttaagtgaaa aataggttta tatagttaag ttttttgaaa caaccaagta tgtattttat
     8041 taaattaaaa taaaaataaa tttaaaaaga gattgtgtca aattataact ttctatttta
     8101 aagaaagata tttgcttgtt gataaaacta caaatatatg atttgaagca atctctttaa
     8161 tttttggagg tggaaaatga aagttttcaa tcaattggta ataattttat ctatctgggt
     8221 aataggagag tatataagtt cctttataca aggaattata cttattcctg ggagtatagt
     8281 tggtatgcta ttattatttc tactactaca atttaaagtt ttagacttga gtagtataga
     8341 aaatgtaagt ggatttttcc tcgataatat ggctatattt tttattccag caggtgtatc
     8401 acttataaag tcattagacc taataaggga aaatgtatta gtgcttttat tagtcatatg
     8461 tttaagtaca ctaatagtaa tgtatactac tggaattatt gtggagaaaa tgataaaaaa
     8521 gaaggaagaa ggacaaaaaa atgtataatg tattacagac accagtattt ggtataatag
     8581 taacaatagt ttttttcaat ttaggaagat acattcaacg aaaaacaagt aatcctatat
     8641 gtaacccttt attaattgca atcgtaggaa taatattgtt tttaagcata tctaaaatcc
     8701 cttatgagaa ttacaagata ggagctgata gtctaaattt ctttttaggt cctgtcacag
     8761 tagtacttgc agttccactt tataaacaat ttgaattatt taaaaagcat atgtttgaga
     8821 ttattgtagg aataggttgt ggtatagtga tttcttttgt atctgtattg ataattggca
     8881 aaatagctaa ttcagatgta agtataataa attcactaat tccaaaatct ataacaacac
     8941 caatgggaat atctttagca aattctttaa atggtgtaga atcaataaca gttgtagcca
     9001 taatttttac aggtattttt ggtggtatgg ttgcttctac agtattcaaa ctggggaata
     9061 taaatcatcc agttgctaaa ggaattgctc ttggaacatc tgcacatgct ttaggaacta
     9121 caaaggcttt tgaacttgga gaaatagaag gagctatgag tggtgtttct attggagtct
     9181 ctggtacaat tacagtaata ctaattccaa ttataatgaa ttttatataa aaaataaaat
     9241 taagacgagt aacttgaata cttaaaacaa aataaaataa aaaaaattta aaataaaaac
     9301 tgaacgaaaa aaatgcaaat aatgaaataa aaaaatcatg tactgaaatt ataaaaagaa
     9361 aaatttcatt taggaattaa ttataaagag acaattacta aacttgaatt tgctaaaata
     9421 aaataaatta ctgcgaaaat agatatttag aaaaaaatac tataaattta ctatattgca
     9481 agttaaataa taagcagtat catattatgc tagaaaaagt ttcatttaag gcataaatga
     9541 gatgatttga acgtttttaa agattagaat ataaaagatt gcactagaca tatatgaaaa
     9601 agataatata atagatgatg taatagatga cagttcaaga aattataaaa aaaatttatt
     9661 tgaaatatta tctattacat caggaataat atatgtaaga gtaagctttt aaaattctaa
     9721 agatttagaa agattgaagg gagaaagaaa tgagtgaagt taagaatacc ctacaaaaac
     9781 atccagtttt aagatattca acaatgctat ttgggatgtt gatgacatct ataggaataa
     9841 atgggttctt aagaccagca catctactta gtggaggtgc tacaggtata gcaacatcta
     9901 taaactattt gacaaatata aatgtaggtt tattaacatt tttaatcaat attccaatat
     9961 ttatcttagg gttcatatac cttgaaaaag agttttgtat ttcaagtttg gtaaatatga
    10021 ttgtattttc attattatta ggagcaactc aggaaataag taatattata cctatacatg
    10081 atatattgct tcaaagtgta tatggtggta tattatctgg tttaggagtc ggagttgtat
    10141 ttagaactag gtcttctcaa ggtggtacag atataatagc tgcaatacta aaaattaaga
    10201 agaatataga aatgaaagac actgctttag caataaatgg cttgatagta cttacaggaa
    10261 gttttttatt tgggttggat ttagcattat acactcttat aggtctattt ctaaatgcgt
    10321 attcaatgag ttttattaaa gatgcaatga attaccaaaa atctgttatg gtaatgtcaa
    10381 atgaagtcga tttaatagca gaagatataa tgaagagttt agttagaggt gtaacttttt
    10441 tagatgcaga aggtgcatac actcatcaaa aaaagaagat tatatataca atagtttcat
    10501 caaatgagat accaaagata aaagatatag ctttgaagta tgataaaaag gcctttatat
    10561 ctgtaaatga tgttacagaa gtaaaaggaa gagggtttaa agcaaaagat ttatagaaaa
    10621 ttttacaagc tgggagttta taatgtaaat ttgtatatta tatctctcag cttatttaaa
    10681 tttagtatac gaaaataaag gttttattaa tgatttgtgt atcactttaa aagatatcat
    10741 tttaaaatta aaattgaaaa aattacttaa tatgtagaaa acatgaccac tattaggata
    10801 taataaattt aatagattta gtaaaatatc taggaataac gttggaggaa tggcattatg
    10861 aatttatcta aagaatatgt taatttgtat gataattatt tgaaatatat aaatgaagta
    10921 aacaaagaga ttagaagtat aaagaacatg aagaaacgtg ttttaaaaag gggcgatttt
    10981 gctccagaaa atgaagtatt agaattggaa ggaaaaacaa tacgcgatat agatgatgtc
    11041 gatacaaaaa aaccaaaaaa taaaatctat aaattttcaa atggggatgt atatgtaggg
    11101 aaattcttag agggaaagat ggaggggaaa ggctcatata ctttttttgt agatgaagga
    11161 acagttatgg agtacatagg agaatttaaa gaagataaaa aaaatggaaa aggaaacttt
    11221 atattttcaa atggaaatga atatataggt tattttgagg aagatatgat gagcggtatt
    11281 ggaaatatgt tatatagtag taaagatgaa tatattggaa cttggaaaaa cggaaaaaaa
    11341 gatggaaaag gtatttataa gtggagtgat ggatgtatat atgcaggtga atttaaaggt
    11401 ggaaagatgg aagggtacgg tatttgctat aatagtaaag gggaagtact ttatgaagga
    11461 gaatggaaga ataatttgat tcatggtaag ggaacttata tatgggaaaa aggtaaaaag
    11521 tatataggtg aatttatgca tggtaaaaaa catgggcaag gaacttttta tctaaataat
    11581 gaattggttt atgaagggac ttggaaattt gataagccat ctatatttga tagaacatta
    11641 gatgaaatat tttccttgag actgtagctt tatctaatta gtagataaag cttttttcta
    11701 atacttactg taaataggta tgaatgatat tgattagtat acactatcaa tattaactta
    11761 ataaatgcaa tattaaaaaa ttttgattga ttttaagaag gagagatata ttgaagagat
    11821 taattgcttt agtgttagta gtacttattg ttttatttta tagtcctgaa ttattaaaag
    11881 aaggatatga tttaaaagaa aagttttttc aaagtgaaat atggaatgaa tttttagaca
    11941 aaataaatat aaatgataat tctaagaaag ataaaaaaga aaaaagtcaa aatttacaat
    12001 caaataatac tgaaggggag aacttaggag agtcagatat aaagaatttt gaaaaaataa
    12061 gcttaggaga taatttaagc tatgttttat caagtatcgg caaacctggt agaattgata
    12121 ttagtgaata tggttttgat tggtatgtat ataatcaata tggaaaagaa tttgcaatgg
    12181 ttggacttga aaatgatgaa gtagtagcac tttatagtaa ttctattaat tcatgtgaaa
    12241 atcaagatat aaaactaaat caagatagac aaacagtgag aactaagatt acaccattaa
    12301 aatataagag aaaaggaaat accagatata taataaactc agaaaatcaa tatgatataa
    12361 taagtaagga aggtaagtat attacaattt tctacgatat tcatgaagaa aatagagttt
    12421 gttcatattt aataattgat aaaagcactg aagatgagtt taaaaattta tatccagatg
    12481 atagcgaaga acttaaaaaa tgttttgaac ttgaagttat agaccttgta aatagtgtta
    12541 gaaatcaaag agggttaaac tcattgagat acagtgaaca ggcaactttg agttcaagaa
    12601 aacatagtga agatatgaga gataataatt tttttgacca tgtaaataag aagaatgaaa
    12661 ctccatttga cagaatgaaa agagaaggta tagtatatac ttcagctgga gaaaacatag
    12721 cagcaggaca gattaatgca atatatgcgc atgaagcttg gatgaactct gaaggacata
    12781 gaaaaaatat tttggggaac tataataata ttggagttgg agtaatattt ggaggaagct
    12841 ataaaacata ttacactcaa aatttttata aataactaaa atatttgaaa ggaagttaaa
    12901 aaatgacatc atggaaaaaa aagacagtat ataagtgctt aatagcagta gtcttgtttt
    12961 gtggaatagt gttaatatct aattttagta aagtttctgc tttgatgatg gatactaatg
    13021 gaaatgtttt gattaaacat ggttcaaggg aaaaaaagct aatagcaatt acctttgatg
    13081 atggtcctca tcctaaagaa actagtcaag ttttagatgt attaaagaaa tacaatgtaa
    13141 aagctacttt ttttatagct ggaaaacatg caaaatggta taaagaacca ttagtaagag
    13201 caagtaaaga aggtcacgag ataggaaatc atactttcaa tcatcctgat ataagtaatt
    13261 taagttctag tcagatagaa gaggagattg ttaagtgtga agacattttg aaagaagtaa
    13321 ctggaaaaaa accgacatta tttagaccac catttggaag ttatagagaa aaagaattaa
    13381 tagaaattgc aaaaaagcat gactataaag ttgttttatg gacaggtgta gatgtgaaag
    13441 attggaaaaa tccaggagct aatagcattg cagataaaat aattaataaa gtacaaaatg
    13501 gagatataat acttttgcat gattatgcaa ctaatgatac agttgaagct ttagatatgt
    13561 ttattccaaa aatgattgaa aaaggtttta aatttgtaac agtttctgag ttaataaaat
    13621 aatttttagg ggggagattt acagtatgat tcttatgata gacaattatg attcttttgt
    13681 atacaaccta gtccaatata tagaagaatt aggggaaaca gttgtagtaa aaagaaataa
    13741 tgaaatcaaa ataagtgaca ttgaagaact aaatccagag gtaattgtgt tatctccagg
    13801 accgtgttct ccaaaagaag ctggtatttg catagatatt gttgagcatt ttaaggggaa
    13861 aaaacctata ctaggaattt gtttaggtca ccaaactatt ggacatgttt ttggaggaga
    13921 tattataaaa gcacaacagc ctgtgcatgg aaaagtatat agtatcaacc atactaataa
    13981 aggagttttt aggggactta aaaatccttt aaatgtcact aggtatcatt cactaatagt
    14041 tgactccaat acagttccaa aagaattaga aattacagca attactgata aaggggaaat
    14101 aatggggatt aggcataaga aatatttaat agaaggagta cagtttcatc cagaagctat
    14161 tttgtcagag tatggtcatg aaatgcttaa aaattttata acagaagcta gagaaagggt
    14221 acatatgtaa tatgataaga gagataaata ctaaattaaa ttcttttgag atatttacta
    14281 tttttagaaa tgaacatgac agttttatat tagacagtgc tatggacaaa gaaaaattgg
    14341 gaagatattc ttttataagt agccaaccat ttaaggtatt aaagtataaa gatactgatg
    14401 aaaatccttt agaagtttta aaagaagaac tacataagta tagggttgta aatgatacta
    14461 atcttccatt tgtaggaggt gctgtgggat atttatctta tgatttaggt aattacatag
    14521 aaaatttacc tagaactgct gtagatgata ttgagatgcc tgatatgtat tttggatttt
    14581 ataatcatgt aatagttata gaccatcttg tgcaaaaaac ttatatagct actccaaaca
    14641 tagatatcga attagaagaa aagataatag atgatataga acaaagaata ttaaaagaag
    14701 agaaaaaagg tatagatagc atatgttatg aagaaaaaga agtaacacct ataagactta
    14761 aatctaattt tacaaaggaa gaatttaaaa atgcagttca aagtgttaga gagtacataa
    14821 gacaaggaga tatatatcaa gctaatttaa cacaaaggtt cagtggagaa acagaactta
    14881 caagctttga attgtatagg gacttaagaa gatttagccc agcaccattt ggggcattct
    14941 taaactttga agatgcacat atattatcaa attctccaga aagatttata agatgtgtta
    15001 ataagagaat agaaactaga ccaataaaag gtactcgtcc aagagggaaa gataaagaag
    15061 aagatttaag acttcaacaa gaacttagaa atagtgaaaa agatagagct gaactactta
    15121 tgatagtaga tttagaaaga aatgatattg ggagaatatc taaaactgga agtgttaaag
    15181 ttccagagct atttgtaatt gaaccatatg caaatgtaaa tcacttagtt tccacagttg
    15241 taggtgagtt aaaagatgat aaagatgcta ctgatgtaat aaaagcaact tttccagggg
    15301 ggtctataac aggagcacct aagattagag caatggaaat tatagacgaa ttagagccaa
    15361 cgcaaagaaa tgtatatact ggttcaatag gttatatagg ttttaatggt gatatggatt
    15421 ttaatatagc aattagaaca ataatcaaaa atgacaaaaa agtctatttt caagttggag
    15481 gaggaatgac ttgggattct gacccagatg aagaatatca agaaacactt gataaagcta
    15541 aatccataat gaaggctttg aggggatatt atgaagaata atgtaggttt tgatagtagt
    15601 ttaagtaaat ttggaattgg tttgtttgaa actattagag ttaaaaatgg aatagcagta
    15661 gatttagata tccatataga aagaatgcta agttcaataa attgtttgga tttagatata
    15721 aaatataaaa aagatttctt gataaatgaa attgttacat atataaaaaa agaaaatgtc
    15781 attaataaag cacttagaat tactgttttt gatgaaggtt ataatatatc tattagggat
    15841 attccatata atcaagagac gtatgagaag gggtttaaat tagctatatc tccaatagtt
    15901 agggggaata gtttaattca tagacataag actactaatt attttgaaaa catatatacc
    15961 aaaaatatcg caaataagac tggttataat gatggtatat ttttaaattc agaaggtgta
    16021 atattagaat gttctatgag caatattttc tttgtaaaag gagaaagagt gtacactcct
    16081 agtgatagat taccgatatt aaatggaata ataaaaaaaa ggattataga gatttgtgac
    16141 gaattacata tagagttgat agaaaatgaa attaatatat cagaaattag tagttttgat
    16201 tttgtttttg tcacaaatag tcttatgggc gctataaagg ttacagaaat tgacaaaata
    16261 aaatttaata aagaaaatgt tgtttttgat aaaatagtga agttattaga ataaattaaa
    16321 atattaacaa tttgttggag acaaaagatg ataggttgta tttatgaagt atacaatgaa
    16381 gagataacta aaataataga aaaccaaaac actttggctg tatatttagt gggttctagt
    16441 aaagacgtag attttactct ttatgatgta gagataaacg atattgatgt gttcgttttt
    16501 gtaaaagaag gggaaaaaca agagagaata ctgtttaaaa aaaagggaat agaatttgat
    16561 gtaaattact tttcaaaaga tggtgtaaaa aaattattat caaataggga atatttcttt
    16621 gtgaaagaaa tgaaagatgc taaagtactg tttgataggg agaatatatc acatattata
    16681 aaagatataa gtagaaatat ttacttagaa ggacccagta aaatgtcatt agaggaaaag
    16741 tcttttataa agcaagatat tggagcaaaa atatcgaatt taaaaaataa agaaaaattt
    16801 gatgtttttg aatatcattt tttgacgaat ttatatttaa aagatataat aattgggtac
    16861 tttaatataa atgataaatg ggtaccaaaa gacaaaaaat tgctaaaagt tttaaaaaaa
    16921 gaaaataatg agctttttga actagtgtca aaagtaagtg aaaattatga ctatcaaaga
    16981 cttttagatg tttataatta tatttttaaa gaaattgaga caaaagaggt tataaaacta
    17041 atcttctagt cacaaatcaa tggggggagt ttaaatgaat aaagttgata aagaaaagat
    17101 acaacatgca gtaagagaaa tactagaggc aattggggaa gacccagata gagaaggtct
    17161 aatcgaaact ccaaatagag ttgctaggat gtatgaagag atattctctg gtcttagtga
    17221 agaaccaaga gaccatttaa aagttctttt tgcagatgaa aaacatgaag agttagtatt
    17281 ggtaaaagat attccatttt attcatgttg tgagcatcat ctagttcctt tctttggaaa
    17341 agctcatatt gcttatttac caaaaggagg aagacttaca gggctttcta aacttgctag
    17401 agtaatagat acattggcaa aaagaccaca attacaagaa agaatcacta aaaatgctgc
    17461 agatataata atggaagaat tacaacctta tggtgtctta gtagtagtag aagctgaaca
    17521 tatgtgcatg acaatgagag gcgtaaaaaa accaggttca aaaactgtta catcagcagt
    17581 gagaggaata tttgaaaagg acattgcgtc tagagccgag gctatgagtt tgattactat
    17641 gaagtagcaa tttattatag atataatcat ttattgagat aaaatttaat ttaagggagt
    17701 gtcattaatt gtgtttgatt atggtaaaag aacttatata atgggaatat taaatgtaac
    17761 tccagatagt ttttctgatg gaggagattt taataacttg gacatagcaa tacaacatgc
    17821 aaaagatatg gttgaccaag gtgcagatat aatagaccta ggaggagagt ctacacgccc
    17881 aggtcatagc tatgtagact cagacgaaga gctaagaaga gtaatccctg taataaaaaa
    17941 acttaaacag gaattggata taccaatatc aatagacact tataaggcag atgttgcaga
    18001 agaagcatta aaattaggtg tcactatggt aaatgatgtt tggggtctta gaaaagataa
    18061 aaatatggct tctgtaatag gaaaatatga tgcagaagtg tgtataatgc ataatcaaga
    18121 tgggacaaac tatgacaaag atataatgga gtcaataaaa gactttttta aggtaagtat
    18181 agaaatggca atgagttgtg gtgtaaaaaa agaaaaaata gtacttgacc caggtgtagg
    18241 atttggcaag gattttgaac aaaatattga agtattgaga agattaaatg aattaaaaga
    18301 tttagggtat cctattttgc ttgggacatc cagaaaatca gtaataggaa aagtacttcc
    18361 agttgagcct aaaaaaagat tagaaggtac aatagctact actgttcttg gtataagaga
    18421 tggcgtagat atagttagag tacatgatgt ttatgaaaat ttaatggcag ctagaatgac
    18481 tgatgcaata tatagaaagt aggtataata taatggataa aatattactt agtaatctag
    18541 gattctatgg atatcatggt gttcttaaag aagaaaattt tctaggtcag aaattctttg
    18601 tcgacatgga actttatatt gattcaagag aagcaggtct tagtgacgat ataaataaat
    18661 ctgtaagcta tgccgaggtt tacaatgttg ttaaagatat tactgaaaat aaacaattta
    18721 atcttttgga ggctttagcg gaaaatattg ctgaagaagt cttgaataaa tttatactta
    18781 taaatggagt tatggtcaga gttagaaagc ctgaggctcc tgtaaatggt atttatgact
    18841 attttggtgt tgagataagg agaacaagag atgaataagg cttatttggg aataggtact
    18901 aacatgggtg atagatttga taatttatca agagcatgtg aacttttgaa aaatagtgat
    18961 tctatatata aagtaaaaga atcaagctta tacgaaacaa aaccatgggg atatacagaa
    19021 caagcagatt ttctaaatat gtgtgtagaa atagaaacag agtttgaacc ttatgagttg
    19081 ttagaatatt gtcaagaaat agagagagaa ctacacagag agagaatagt tcattgggga
    19141 ccaagaacta tagatgtaga cgtactattt tttaatgatg tagtttccac tgatgaaaga
    19201 ctaacaatac ctcatccaag gattcaagat agagctttcg ttttgatacc acttatggat
    19261 ttaaatgaag aacttataat aaatgaaaaa acaataaaag aacatttaaa cctcctatct
    19321 gctgaagaaa gagaagaagt aaaggagctt gtaggttatg aaagaaaacc tatttaataa
    19381 taattcaaag atagagataa atacaaataa caaaaagata actgtaaaaa aagataaact
    19441 tataatagct ggaccatgcg caatagagag ttatgagcaa cttttggaga ctgctaaatt
    19501 tgtaaaaagt caaggagcaa acattttaag aggtggagca tacaagccaa gaacatcgcc
    19561 aaattcattt caaggattaa aaaaagaagg tcttgaaata ttaaaagcag ttaaagacga
    19621 agttggtatg gctgttatta cagaacttat ggatgtaaga gatatggacg aactgtatag
    19681 tataagtgat ataattcaaa ttgggtcaag aaatatgcaa aactttactc ttttaagtga
    19741 agtgggaaaa caaaataaac ctgttatgtt aaaaagagga atagcatcca caattactga
    19801 atggataggt gcttctgaat atatagcaat agaaggaaat agtaatatta taatgtgtga
    19861 gagaggtata agaacgtata atgattatac tagaaataca ttagatttag ctgcggtacc
    19921 cattattcaa aaggaaacag gtcttcctgt tgtggtagac ccaagccatg ccactggagt
    19981 tagatatttg gtaaaaccta tgtcactggc atcttttgca tgtggagcag atggaataat
    20041 ggtagaagta catcctgacc cagaaaatgc tttgtcagat ggtgctcaat ctctttgctt
    20101 taatgaattt gaagatttaa tgaaatcaat taataattat taaaagaggc aagagcctct
    20161 tttttattcg taatcgttgc taaggtaaat ttataagtta taatttgata gttagattat
    20221 ttaatataaa taaagattta atattgatag aattaatata aaaaataaaa attttttgat
    20281 taataagaag gatatcaata aaaaaagtcg aattgtgtat attgagtgat tgacaaattt
    20341 agaatatagt gtagaataga aatgttaaca caaaaaatta caccagaagg tgataatatg
    20401 aatgatataa aagacattat tgaagaaata aaagctagat gcgatatagc aagtataata
    20461 tctgaatata tgaatataaa acaatctgga gctaattaca aaggattatg tccatttcat
    20521 ggcgaaaaga caccttcttt ttatataaat acatcaaaac agatttataa gtgttttggt
    20581 tgtggtgaag gtggagacgt aataaatttt gtcatgaaaa tggaaaattt ggattttatg
    20641 gatgctgtaa aaattttagc aaataaatgc ggtatagaaa ttaacactaa catgaatgaa
    20701 gaaactagga ttaaaataga aaaatctaag aaatttcaag atattcatac ggaagcagct
    20761 agattttatc tttcaaactt attgggaagc aaaaatcttg ggtatgaata tttaagaata
    20821 agaggactag atgataaaat tattaagaaa tttggtttag gattttcttt ggattcatgg
    20881 aattcactta tgaatactct aatcagtaaa ggatataaaa aacaagactt attagagtgc
    20941 ggtctaattg caaaaaatag agatggaact aattgctatg ataaatttag aaatagagtt
    21001 atgtttccta tattcgatta tagaggaaat ataataggat ttgggggaag agtattagac
    21061 gattctttgc caaaatactt aaattcttca gacacattaa tttttaataa aaaacagaac
    21121 ttatacggat taaattttgc tagaaaaaat ttagaaagta aaactatagt cttagttgaa
    21181 ggatatatgg atttaatttc actttatcag tatggtataa aaaatgtagt agcaaccctt
    21241 ggaacagcct tgactgaaca acaaggcatt ttaataaaga gatatgcgga cactgctatt
    21301 atatcttatg attctgatga agcaggaata aaagcgactt taagagctat agatatactt
    21361 actaaattag gtatcaatgt aaaagtgtta gacttaaaag atgctaaaga cccagatgaa
    21421 tttgtaagaa aatacggact tagtgattat aaaaaagcta tggatgtttc tactcactac
    21481 ataaaatata aaatagatca tcttaaaaaa gaatttaaca ttcaaaaaga tgaagaacga
    21541 gtgaagttcg caaaagaagc ttctaaaatt ataaaacaat tgacaagtcc tgttgaaatt
    21601 gatttttata caaagtattt aagcaatcaa atagatatta atgtcgagtc tataaagaga
    21661 gaagtttatg gtaaaaatta caataagcca tataataaca aaaatcaaaa gaaaatagaa
    21721 gaaaaagtaa ttgaaaaagt cgaagtcaga caagacggaa aacaattagt tgaagaaact
    21781 ctaataaaaa taatgttaga agacaaaaaa attagagaaa tagcgttact taaagtagaa
    21841 gaaagtgatt ttttattgaa agaaagtaaa gaaattttaa attacatgat taaaaatcaa
    21901 gaattggaca aaataactat tgacaaattg aaaagtttaa acatatccga agaatactta
    21961 aaagaattaa attcaatttc tttgaatagt ataaacttag agaacacaaa agaaatagaa
    22021 ggaataataa caaatatcag aaaaaactca ttagaagagc aaattaatag cttgttgaga
    22081 gaacaacaag aattagaaaa taataatgat atgaaagagg tagatggtag agttatggaa
    22141 attgctttaa aaatagttga gataaataaa attttaaaga gcttgtagag aaaggaggtt
    22201 tgagtgtgga aaataaatcg aataaaaaag agttaaaaaa ggttactgct aagacattaa
    22261 tagaaaaagg aaaaaagcaa ggttctctga cacttgcaga gataatggaa gctttttcgg
    22321 agactgaact tgataaggat caagtagaaa atctttatga gactttagga aatttgggaa
    22381 tagaaataac agaaacaaaa aactataaag ctgatataga tttttcggtt gctgatgacg
    22441 atttaagtat aggccactta gatgaagatg cagaggcaat ttcacatgat gactcttctg
    22501 caatagaaat agaaactgtg gatttatctt taccaaaagg gataagtata gatgaccctg
    22561 taagaatgta cttaaaagaa ataggaaaaa ttcctctact taaaccacat gaagaagtag
    22621 aatttgctag aaggatgcac gaaggtgatg agatagcaaa acaaaggtta gttgaagcaa
    22681 acttaagact agttgtaagt atagcaaaaa gatatgtagg aagaggtatg cttttcttgg
    22741 atttaataca agagggaaat ttaggtctta taaaagcagt cgaaaaattt gactatacaa
    22801 aaggatataa gtttagtact tatgcaacat ggtggataag acaagctata acacgtgcta
    22861 ttgcagacca agctagaact ataagaatac cagttcatat ggtagagact ataaataagc
    22921 taataagagt atcaagacaa ctacttcaag aacttggaag agatccaaaa ccagaagaaa
    22981 ttgcaaaaga aatggaaatg acagaagata aagtaagaga aataatgaaa atagctcaag
    23041 accctgtgtc tttagaaaca ccaataggag aagaagaaga tagccattta ggtgacttta
    23101 ttccagatga tgatgctcca gccccagcag aggcagcagc atattcttta ttaaaagaac
    23161 aaatagaaga tgtacttggt tcattaaatg atagagaaca aaaagtatta aagcttagat
    23221 ttggtcttga ggatggtaga gccagaactc ttgaggaagt tggaaaagag tttgatgtaa
    23281 ctagagagag aataagacag atagaagcaa aagcactaag aaaactaaga catccaagta
    23341 gatcaaaaaa acttagagat tatttagact aaaaagatac atcattgatg tttttgtgat
    23401 aaatgccatg agataagtga tatctcatgg ctattttgta gtaaagattt aattttattt
    23461 ttgtagttat ttcagtctaa tatctataac ttggattaaa tcctataaat aaagggaatt
    23521 gaaaggagaa ttatatttga aattaacaga tagattattg aaaatagcat cattagtgtc
    23581 agatggtaaa aaaatagctg atataggaac ggaccacgga tacataccag tatatttgtt
    23641 aaaagaggga aaagtaccat ttgcagtgct agcggatgtt aataaaggac ctcttgacaa
    23701 tgcacataaa gaagtcatac agaacaatct tttagataaa gttgatttac gattgggttc
    23761 tggtatagag atacttgaaa taggggaagt agaagaagtg ataattgctg gtatgggtgg
    23821 aatcttgata agtgagcttc tcgaagctaa aaaagaagta gcacataatg tagaaaaatt
    23881 aatattacag ccaatgcaag cacaagaaga acttagatat taccttttaa acaacggata
    23941 tgaaatttta gaagaagttt tagtgagaga agattttaga atatatgaga taattgttgc
    24001 caaatataca ggtaaaaata ctataataga agatgagata tattatgagg ttggaataaa
    24061 gcttttagaa aacaaagatt ctctttttaa tgattttata gaaaaaaaga taaagacata
    24121 tagttctata gttaataaat tagaagggaa aaatggggaa gctatagata agaagagaaa
    24181 ggaaagcgaa gttgcaataa aaaaacttga aaatttaata aaataaatag gaggtatata
    24241 tgctattaaa gagtctaact agaaaaattg aaaaaaagta tcctctaaac ttagcagagg
    24301 attgggataa tgtaggtctt atagttggag attttgatat ggatgtaaaa aaggtattgg
    24361 tatctttaga agctaatgaa gatgttattg atgaagctat atccaaaaat atagatttaa
    24421 ttgtgacaca tcatcctttt atatttggga aaattaataa aattaatagt ggagatttaa
    24481 aaggcagact tatccaaaaa cttattaaaa atgatatatc actttattct atgcatacga
    24541 attttgatat agcatttgat ggtctaaatg actattttat ggaaattatg gaatttggta
    24601 attcaaaggt tttagatata actaaaagcg aaaatttgta taaattagca gtatacgttc
    24661 cacataacta ttcagatgaa cttagaaagg ttttaagtaa ttcaggagca ggtcatatag
    24721 ggaattatag tgattgtacg ttttcaacag aaggtgaagg acaatttaag ccattagaag
    24781 gctctaatcc gtttcttgga agtgtaaatg atatagaaac tgtaaatgaa gttaaaattg
    24841 aaactgtagt accacagaag cttttaggtg gggtaataag ttctatgtta gatgcacatc
    24901 cttatgaaga agttgcatat gatttgtaca agttagaaaa taaaggtgag atatttggac
    24961 taggtagaat aagtaaactt gataaaagta tgacattaga aagtctttca aggaaaataa
    25021 aagagaaatt aaatatgaaa cacataagag tagttggaaa ccttagtaca gacataacga
    25081 aagtagccgt tgtaacagga gctggttcag aatttgtaaa aaaagctaag agacaaggtg
    25141 cagaagtact aattacgggt gatgtaaagt atcatgaggc acaagatgca cttgatattg
    25201 gaatgtgtat agtggattgt ggtcattttg atacagaaga tatattcaaa aatgttatga
    25261 agagattttt agatgaattt agtgaaattg aagtaataaa gagtaatgta tatttgaatc
    25321 catttagtac tatttagcag tgtttgataa taaaaatact atataaaaaa taaatttgag
    25381 tttaattagg gggataatga tgaaagtagc agtaattttt catagtgttt gtgggagtac
    25441 atatttatta gctagagaat ataaagaagc gcttgaagaa atgaatatcg aggtagatat
    25501 atttagagta agtgacgaag ttgcaaaaac acttcctcaa tattatttga taaattccaa
    25561 agaatacaaa gacgaatttg aaagtataaa tgtaattaaa tctggaaaag aaatattaga
    25621 ttatgatgct atatttatgg gttcaccaac atattatgga aatgtatctg gaccaatgaa
    25681 gatgtttatg gatagttttt ctgatatttg ggtaggtgct cctttgagtg gtaaaatatt
    25741 tggttgtttt gctacagcag gttcacaaca tggaggagga gaattagctt tgcaagctat
    25801 gaatatattt gcccaacata tgggaatgac tcttttgtca gttccatgtt cagtaagagg
    25861 aggttatcca gcgtatggaa tacttcatat agcaggagat aattctgata taaggccaaa
    25921 tgatgatgcc aagataggta taagagatta cctaaaaaga cttaacatat aagaatatca
    25981 tataaaaata cctaaaagcg actgtatata cttttaggta tttttactta attataaacg
    26041 tgaagcttta ccagaaatat ggtcaatttc tattttaaca acagttgtta agttcatatc
    26101 cttctctatg tacttcatgc cttcttcaaa gaatccttta gaatattttt ttattatttc
    26161 tacaagagca tttttctttt cttcattttc tacagtgcaa gcttttccaa aaactatagc
    26221 actagaataa gtagtactaa atttatctgg aataacactt tcattagcaa ctactaaaaa
    26281 agaaactttg ttgtttttcg atatattatc taacttatgc ccatttcttg cacaatgaaa
    26341 gtaaatagaa tcattaaagt atacataatt tacagcaact ccataaggat aaccattttc
    26401 agatatagtg gaaaaagtac caaattcacc attttttaat acttcaacag tatcttcttt
    26461 tgtcatttct ctttttttca atctcatttc tttaaacata atatcatcct ttctaatttt
    26521 gatgcttaaa ttaaatttta gaacatttat aaagattatg ctatgttttt tacaaatttt
    26581 aagaaaaaaa tatagtttaa gataaaaggt agtgaaagag aagctatttg gaggtttatt
    26641 tatgatatta ttgttagagt agtacaaata atactaataa atttaatagc tggtttcttg
    26701 ttatttaagg tagtaagcat gtctataaga aggggaagtt atcgagggta gaagttgttg
    26761 tttataacga tattataaag aaaatattat tatataaaat aataaatagt aaagaggtat
    26821 caagttgaat ataatactta atacctcttc aaaattaagt tataaaagtg ttgtgggaat
    26881 tgtcctatac atggtaagga gataataaag aatgtatctt taaagtctca actaaacagc
    26941 aaaatttatc caacaaacat acaataaatg cttctttgta tctaggtggc ttaacagtaa
    27001 gtggccacat gtgttttaaa attatgtccg tttctttttc atttatttga aaaaataaag
    27061 ttgcattttt taatgcttct ctagggtgag taaatccatg taatcctttt ctatcacctt
    27121 tgtaatgcca atcataaaga aaaaagtcgt gtagtaaagc tcctctaata acactttgaa
    27181 tatcaacact taagtgcaat tttttacata atagataact ataatatgct actgacaaac
    27241 tatgttcaag acatgaaaca ttgccatgat gaggaaatgt tctcataagt tgaacttttt
    27301 cagaagaaag tatctcagaa gcacattcat taaaatattt gatttcagct tttgacaaca
    27361 ttatgaaaat ctccattctt agtaatatat aaatactata cttcaacgat aatatgaaat
    27421 atttcttatc ctacaaaata taatacccaa taatacaagt aagatacctt ttatggtatt
    27481 ttaaagtaag agagatttta ttaggctaaa caatgaaaaa attaaaagta aagctatagc
    27541 aatatatagt atttttccaa gaggattgcc aaaatttata gtccatccaa taccaaacct
    27601 tttattaacc attaatgatg ggtcatttgg attattatac atgatgcctg ctatccaata
    27661 cttctcatct tcttctggag aataagatga gttgaatttt aaatttgggg aattaatata
    27721 tgttgctatt aaataaatta taactaaaag cgttgtaata ttcatagcag ttaatagttt
    27781 tgtttttatt gataaaagag tagtaaaaaa ctgtgttgtc attataatca tcaatattaa
    27841 aaacaaataa ccaattttat ttaaatattt aatgttttca actctacttt cttcaattct
    27901 atttgtatct atcttaatcc ttgactttat ggatccaatg gaagttatat ataataaaac
    27961 cattaaacaa aagtcagtac cgattaattt aaatacatta agaaatgatt tatctataaa
    28021 aacatcagga gacccattta tgttccaatg agtggcaatt gagtctggca attgattata
    28081 attaaatgct ataaaaatac tcattcccaa agtaaatagt atcggtatta aatagattat
    28141 tttaaatttt tttattattt tatttttctt atttataaaa tccatatcta ctattaattt
    28201 tgaatctata tctatatgac ctaatttagc ataaatttct gatttaagct tcttagcttt
    28261 tttatgtgta tgaatatata gtatactttc atataataaa aaacctataa taccaataaa
    28321 ataggaaagt tccaatttat taaatgtaaa gaccagaata aaaactaata taaagtctat
    28381 aataaaacct aaagataata gttttttaaa ttctttatct aactttataa agtaattata
    28441 atttttatgc tcttggtcta tagaaactcc atagatgtgt tttttaccta caaaataggg
    28501 tataaaataa aatgtagcat acatcaaaat tagaactgga aatattgtaa atagacattc
    28561 aatcttactc ataatatttc acctcatact tgtcaaataa acttgaactt agtgacaaaa
    28621 attcttcttt actaatacct gataaataag attttgccac caataattcc aagtcaagtt
    28681 tatttttttg acgagttgta ttatctattt tcattggtag ttgagctaca atagcacctt
    28741 ttcttctatc aatatttaag aatccctctt cttttaattc attgtaggat ttgttcactg
    28801 tatgtaaatt tacaccaata ttttcagcca tacttcttac agatggaagt gattcattta
    28861 ttttcaattc tcctgaagct atagccttag ttatttcttc tttaatttgt acataaatag
    28921 atttatcact attaaaatct aaatttaaaa ccataaaaaa cctccattat gctttataaa
    28981 tgaaaacgat tgttatatac atagtataac ataaaaaata ataacaaaca caaaatataa
    29041 gtttatattt tgtaaaaatt gtaatttaaa aaataacata atttttatta aaataataca
    29101 gatactattt atagatagaa ataaaaatta gaaaggagaa aaaatatgtt aacatattta
    29161 atgaaaagac aaatgcataa aaaagatgat ggaatgagta aaccaatgat gattacagga
    29221 gctgtaatta caggtgtagg agcatatgct gtatataaaa tggtaaaaag ccataaatct
    29281 aattctcaaa tggtcaatac aaattattat ggaaatagcg attatgatga ttataaatat
    29341 gacgaatttg attatgactg tgattgtaga gacacaaaac aagattatga atatgaagaa
    29401 ttaaaaaaaa aagtaaaaga atttaattca agaagaataa attgtgaaaa ttgtccacat
    29461 gtttctcaag aagaaatgat gcaatatgta aatagtgtta aagatgtaaa ggaagagctt
    29521 gatttacatg aagatgctaa aaaagataat atttacaaag aagatgaata caaaaaatat
    29581 gaagaagaat aagtaatatt ttgtataact gaacaaatag taatgtcaaa ttatttaata
    29641 tctagtttta atatgtaagt tgtaacttaa cttgaaagaa tcagtatcat tctttagata
    29701 gttaagtaac aacttacatt ttatttttat aaattttaat agttctttgt gactattttg
    29761 taattttaga aatattttag tatccagggt aattgtgtat aataaaactt gtatagatag
    29821 atagtatgaa attaagcaga tagtttaaaa ttaaaatgaa aagaaaggaa atagttatgt
    29881 taaagttgtt gatagttgaa gatgatagta ctatatcttt tggaataaag tatgctttag
    29941 aacaagaagg atttagtata gatatatcaa aggatttatc tagtggaaaa gaaatgattt
    30001 cttctaatga atatagcatg atactgttgg atgttacatt gccagatgga actggttatg
    30061 agctttgtca atatataaga ggattctctc aggttcctat aatattcttg actgcttgtg
    30121 atgaagaagt aaatattgta atgggtctcg atataggtgg agatgattat ataacaaaac
    30181 cttttagaat tagagaatta atatccagaa taaaagctgt tttaagaaga aaaggtaata
    30241 cttcagaaga aaataagaaa atacttaaat ttggagattt aagtatatat acattagaag
    30301 ctagagtata taaaaatgat aaagaaatat ttctgacatc agttgaatat aaacttcttt
    30361 taattcttat acagaataaa aatactgttt taagtagaac tcaaatattg gaaaaacttt
    30421 gggatgtaac ttatgatttt gtaaacgaca atactctaac tgtttatata aaaaggctta
    30481 gagaaaaaat aggtgatgat ttatgtgaac caatctacat agaaactgta agggggattg
    30541 gttataaatg gataggaagt gaaaatagtg tttctatata atccagaagt gaagaagttt
    30601 ttaagtaagt atgttacttt gatgtttatt gttatcataa tatctattgg atttagcata
    30661 atcaacgtgt ccttaactaa agagatggtt gtaagaaata atcaggctat aataggaact
    30721 ttatcaagca agtatccaaa cttagaaagt cagatagtag acataataac tcaagggaag
    30781 tctatggaga acatagatta tggtcagaaa atattaatta agtataatta tgataaatct
    30841 attaggataa actctgagcc tattatttct aaattagttt tagagactat caaaataaat
    30901 ataatattag tatgtataat atttattttg atatttgtgt tgattgttag atattttacg
    30961 tcaatttaca atgatttatc agatatgact aagtatgttt actatagttc agagggtaag
    31021 ccatttgata tgaaaaacaa gaatcaagaa gggcagatag gtcttctaaa aactgaactc
    31081 ttaaaaatga cgacaattct taatgaaaag gttgaacttt taaaaagaga aaagatattt
    31141 ttaaacaata caatttccga tatatcacat caattaaaaa ctcctatgac ttctttaata
    31201 atgttgaatg atttattata taatgatgta ccatacgaag taaaaatcga ttttttaaat
    31261 aagataaaaa atcaattaaa tagaatggat tggttgatta aaagcatgct taaattatca
    31321 aaggtagaag caaaagtgat aaattttaaa aaagataagg taaaatttag tgaactcata
    31381 catagagcaa tgctatctat gaaaatacct atggagatta aaaatcaaaa actgactata
    31441 gagggaaatg ataatgtatc ttatattggt gatattgatt ggtccgtgga agcattagtg
    31501 aatataatca aaaattgtgt agaacatact cctgaatttg gaaatataat tataacctat
    31561 aaagaaaatc cattattttc tgaactaata ataaaagatg agggtgaagg tatagataaa
    31621 aaagatatac cacatgtatt taaacgattc tatagaggaa gaagtagttc aaaagaagat
    31681 agtgtaggaa taggtcttgc aatgtcaaaa tctataatag aaagccaaaa tggcgatata
    31741 tatgtaaaca gcgaaaaagg taagggtaca gaatttcata taatatttca taaaatgtat
    31801 gatagtgact aatctgtaat atttaattca ctttatagta atagccaaaa cctaatatat
    31861 gaagtataga atataaatat tggaggtagt tatggaaatt ttaagagtag aaaatctaac
    31921 taaaagttat ggtaaaaatg aaacaaaagt tgatgctata aaaaatgtaa gtttatcagt
    31981 tgaaaaagga acgtttatag caataacagg tccaagtgga agtggtaaaa gtaccttatt
    32041 acatttatta ggaggagttg acagaccaac tagtggtaag gtttatataa atgatgttga
    32101 tatatataat ttaaaaacta aggatttggc gatatttaga agaagaaata taggtcttat
    32161 atatcaattt tacaacttaa ttccagtctt aaatgtgaag gaaaacatat tgcttccagc
    32221 agagcttgac aatagagata ttgatagaga atattttgat gatttaatga aaacattagg
    32281 tctaattgat agagaaaagc accttccaaa ccaagtatca ggaggtcaac aacagagaac
    32341 ctctatagga cgtgcattaa ttaatagacc ttctataata ttagcagatg agccaacagg
    32401 taatttagat agtaaaaatt caaaggaaat attagaattg ttaaaattgt cagtaaaaaa
    32461 atacaaccag acactaataa tgataactca tgacaaaaat atggcacttc aagctgatag
    32521 aattataagc atagaagatg gaatcataaa aagtgatgag gtgatgtaga tgaatttata
    32581 tacatcacta actattagat acttaaaaca aaataaaaga agaaccattg taaccataat
    32641 cggtataata ctttcaacag ctttaatttg tggtatagga aatatatttg aaagttttat
    32701 ggattatcaa atgagagaaa ctataaagaa tgatggaagt ttccatgtta cattttatga
    32761 tgtaaataga aaaaatgtgg aatatgtaac gaaatcagct gaaatagaaa aacatgcatt
    32821 taccaaacaa cttggatatt ctaaattaga aaatagtgaa aatgctatat taagtataaa
    32881 gcaatatgat aaaaatgcaa taaatggata taaagtttct ataaaagaag gtagatttcc
    32941 agctaaggaa ggagaaattg ttttaagtga aagtattatt aatcttatag atgataagtt
    33001 aaaaatagga gataagataa ctttaaaagt aggggatatg tttgactcaa gcaagaaaaa
    33061 gattgatagt atgacatttc atgaaggtga ttatatagca aatgaaaaag atagaacttt
    33121 taaagttgtt ggtataattg aaaaacctgg gtttgaacgt tataatggaa ttgcaacagc
    33181 tatagcctac tttaatccta tgaataatta caatgataca attaatgtat cagtagcagt
    33241 aaaaaatcct aaagatgtgt ataagataag taataaaata agtaaaaata tatattcaaa
    33301 taaagataaa gaagcagttt ctgatatgat aaaatacaat gaacatttat tgaggttaca
    33361 aggtgctagt aagtattcaa atataaatag ctctataaag tctataatta tagcagtaac
    33421 tattttagta atcgtatgta caattgctac agtgtacaat tcctttagta tttctataaa
    33481 tgaacgaaaa aaacagtttg ggatattaaa ttctataggt gctacatcta gtcaaattaa
    33541 aagattagtt tttatagaag gtattataat tagtttaatt ggtataccaa taggactaat
    33601 atctggaaca gtagctatag acttattgtt taaaattata aataagtatt ttactgaatc
    33661 ggttgttact aagatgagtt tgcagatagt gtataatcca ataataatta ttgttagtat
    33721 aataatagtt ttgcttacaa tctttatatc aattctacta cctgcaatat cggcttctaa
    33781 tatttctccc cttaatgtga ttaaaaatac tggagagtat aaagtgggca aaatcaaaag
    33841 ctcaagactt attaaaatga tttttaaaac tgaaggagta cttgcatata aaaatattag
    33901 gagaaataga aaaaaattta taataactct tttttcttta atggtaagta taataatatt
    33961 tatatctttt agtggcttta cattactttt actaagaggt gaagaaatta gaaattctca
    34021 aagaaaatat gatttgtatt taactgcaaa gaacacagct aggtctgtag aggatactat
    34081 aagtgaacta gaagatattg atgggataaa aaactttgaa ttggcaacag gacaatatgt
    34141 gtctataaga gtaagtgaaa ataaaataaa taaatccaag gaagatttaa tcaggaaata
    34201 ttatcaaaaa tataagattg gagatagtta tgaatatgat ttcagtaata atgaattaat
    34261 ttttccagga gattttgcag taaaaaacaa aataaataac ttagtacagg gctcttttaa
    34321 taaagagaga gcaattgaag aaaatggtgt tatattagtt agaaaaagtg caattgaaga
    34381 aaatgggaag aaaggtgtag tagaacttac caactataaa gtaggagata cagtaaattg
    34441 tgaatatctt gatgaaaatg ccttaagtaa aaaagtaaag gtcaagattc ttgctataac
    34501 tgacgaagaa cgtctaggtc ttggttatca aaatatgggg ttacaattta taacttatga
    34561 tgaagttgct aaaaatttaa atttaaaatt aagtaaaagc cttatattta ttgattcagg
    34621 aggaagcatt aagactaaga aggaaataga agctttggca aataaaaata attttaactt
    34681 ttatgatgaa agtgcaagca atgaaagtga aaaacaagat ttaaaggtta ttaaaatatt
    34741 tgtatatggt tttatagtag tgatatcttt agtgagtgtt acaaatattc taaatacagt
    34801 aagtacaagt ataaatctaa gaaaaagaga acttgcagta atacaatcta taggggttac
    34861 tcctaaagga tttaggaaaa tgatatattt agaaagtttt atatatggta tactgtcatt
    34921 gttgtttgga ataccaatta gtattggtat aattctaatt atgaataaac ttatttcagg
    34981 tgtaattgaa ttttctccga ttataccgtg gacagccata gttatctgta tagtaagtgt
    35041 ttttataata acttttatag cagggtatat accaatgagt aaattaaata aagaaaatat
    35101 aatagataat ataagaagag aaagtatata gatataaaaa taattcttaa ctatataaaa
    35161 tattattaaa atcatttaag tggtataaaa tattattata tcacttaaat aattttttat
    35221 ttatataaga aatctaataa taaattacta gaggtattta actatttaga aagaattggt
    35281 attatataaa gtaagagaat atataataag aaagaatatt tgattttatt ggaaaacaaa
    35341 agaaaaagaa ggtgattact atttctcata taactgcgaa gaatatgtat aaaagtttag
    35401 aggaaagaat aaacaagttt ccacaaggtg ctccaccatc tgatacatta tacaaaatac
    35461 taaatgtact atatacagaa caagaggcga agttagttgc tcagcttcca ataaagcctt
    35521 tcagagtaaa aacagcagct aaaatttgga gtgtaagtga aagtgaagca tatagagtat
    35581 tggataaact tgctagtaag gctctgatac tagatataga agataataaa gggaagaaat
    35641 atataatgcc tcctccaatg gctggttttt ttgagtttgc catgatgaga acgagacacg
    35701 atatagacca aaaacttttg gcagagcttt actatcaata tatgaatgta gaagaagatt
    35761 ttatcaaaga tttattttat tcaacagaaa cgaagcttgg aagagtatac gttcaagagg
    35821 aagttttaac taatgataat gaggttagta ttttagatta tgaaagggct actcatataa
    35881 ttgatgaatc aactcatata ggtataagta tgtgttactg tagacataga atgcaacatg
    35941 ttggaaaagc ttgtgatgct cctatggata tatgtatgac gtttgacaat gtagcaaatt
    36001 ctttaataaa taataaattt gcaagacgtg tggataaaat agaatgtaaa gagctacttc
    36061 atcaagcata tgaacataac ttagttcaat gtggagaaaa tgttaggaaa ggggttacgt
    36121 ttatatgtaa ttgctgtgga tgttgctgtg aagcaatggt agcagctaaa agatttggaa
    36181 atcttcatcc agtacagaca acaagtttta taccaaatat aaaccatgaa aattgtgtaa
    36241 agtgtggtaa atgtataact gcatgtccaa tagatgcgat aagcaaggtt aaggaagatg
    36301 gtaaagaata tataaaaatt gatgaggata gatgtttagg ttgtggagtt tgtgttagaa
    36361 attgccataa aaatagcatt atgttattaa agagagatga aaaaataata actccagcaa
    36421 attcagtaca tagagcagtt cttatggcaa ttgaaaaagg gcagttacaa aatttaattt
    36481 ttgataataa tgctctggct agtcatagag ctatgggagc tatactgtca gctatattaa
    36541 agcttgaacc agctaaaaag attttagcca gtaaacaatt aaagtcagta tatttagata
    36601 aattgttaag tatgaatgat aaatagaaac taacttaaaa aaggcaaagt tttagactta
    36661 tctatatctt aataaactta tcatttaata tttaaattta atttttacac atataaaact
    36721 tataacttaa ataaaaaaag gctatattca attaatatta cttgaataca gcctttttta
    36781 gttatgttaa gctatttctt ttatttgctt aaatgagttt tcatttccat ttccaccaat
    36841 ttgtgttaca gatttaaatc tcatagtttt aaatagttct ttttgattat aatctagtga
    36901 gtttcctact agcattacag gagatttagt ttttccagcc aaaactccaa cagataatcc
    36961 gtctattaaa tcatcttgtt tattcattcc atttttagca acatataaat tgtctatctt
    37021 agaatccttg taaaattctt ttattacttt tgcattagtt tcatttctag tgctacctga
    37081 tattctctgt ggattttgaa gtttactttc tatattttta gaaactgtat attctccacc
    37141 gattacataa gatttatcaa tctttttgtt tttaaacaag ttatttattt cagttatatt
    37201 actattttca ttagtaagta ttataggcat atcattttga gctgatacag ctccaatgct
    37261 tactgcatcg gctaatcctt tttcgccatt aactactgaa atctttgata tattgctaat
    37321 attgttcaat tctttagcaa tgttcataga agtttgatat ctatcacttc ctgaaattct
    37381 agatacagag atattgtttg attttatagt atctaaagat ttttcattta tagaagcttc
    37441 tccaccaact acatatacat ttttaacttt taatctcttt aattcagcca aagttttttc
    37501 atttatttga gagcttccag tcaataatat aggagcgttt ttcttgtatg caaatggtgt
    37561 tgcagccaaa gcatctgcaa ttgctgaatc gtttattaaa actgcatttt cagcattttc
    37621 ccaacctgct tgacttatct taatagaagt ttcatatcta tcagttcctg taagtgcact
    37681 tgcatttact tcatcttgat atgtaacctc aaatctagca tttggacttc ctaagaaaac
    37741 aattacgcct ccttttggca atctaactct attgttcttt gtaactaaag aatagtttac
    37801 aggaacacct ttatcatcat aaacagcaaa agctgaattt tgaggaagat taacttctat
    37861 ttttttattt gcgatatcgt caccaatctt ataccatttt gcataaccat tggattctag
    37921 ttcacaagta aaagattttt ctgctggcaa attagttata gaatcttcac taacatacgt
    37981 tagtgttcca aaagataaat actctgtacc attttcttta tgaagtttaa tgtcggataa
    38041 atcacgacct attacaccag gtatttctat aaaagcattt gagttattct catccattat
    38101 tttagtgtta actatataac ctggagtttc atcagaaaga gcaagagttg ccttcacaga
    38161 accaaacata tagctttgag aagtatattt ttcatctact agatagtaat ctttaccatt
    38221 tctttttttc catgcttctt ttacactatt tgatatattg ttgtcatcta tcttttgagc
    38281 tacatagtat aaacttgctg tctgacctaa tccagggaca tcatcataag agctcatatt
    38341 taaatatgtg atattgtttt tttcttttac aaattttaga cagctgttac ctttttcaga
    38401 tacaaatcta tcttgaccta tgtatacgta tttatcttca ggtccatttt ctatataagg
    38461 agaagatact gttaaagtac cattatcatt aacatctact tttatcatat ttgaagaagc
    38521 ataaagacca ctattttctt ttagagagct aggcatttta acttgttgtg gtttactaaa
    38581 tgttttatca ggttttattt ctttaatttt acctttttct tttaatgcag atagtaagat
    38641 ttcttgtcca attatttcat taagttgact acttccacca gaagaaagaa ctgcaactgc
    38701 catattttca tctggaagaa ctataagatt actgtggaat aataaagagt ctccaccttt
    38761 tgtaagagct tttaaattat actgattaaa tggataagat agcaataggt aagagtttga
    38821 tgctatctcc gtcttttccc ctgctccaca ccgtacgtga gactttcacc tcatacggcg
    38881 ttcccccaag aatctatcaa tcatattatt tcatttccga ttttcttacg atatttattc
    38941 aatttcttaa tttcttgatt gtctaagttt agttccttta tataatattg tatgtgttca
    39001 atattagttg catgaataag tttatgtatt tctggtataa ctaaaatcaa attcctaaat
    39061 ttatcatcac ctcctttgtt gactggtgtg atgtggtgtg cttccatatt ttctactaat
    39121 ggataaccac ttataataca tgttcctttc tgtgctgaat attttgataa cctattgtca
    39181 ttatattcta cacttttatt aagtataggg ttatgtgcta tatagtttag tatattatta
    39241 tctatacaac ctaaccttgt atgtattatt ttccgacctt ctttcgtata atttgatacc
    39301 gtttggttaa atagaaacgg tggggtcgtt ttgattccag ttagaggata catgatttca
    39361 tttagtatat attgcttctt taagttatat ttactatatt tactttcata ttcatgcgtt
    39421 aaatttcctg tagaagttct tagatttttc aagcgattat ataaactgaa agaaagatta
    39481 tatttaatgt tcttaaagtc ttgatataca ttcgctacta ttttataata gttatgagaa
    39541 ccagcaataa ttctattcaa catattgata ttcttagttg ttggaaaatg ctgtatagct
    39601 attaattgtt cttttatatt gtgactgact tgctctttag ctttgtcagt catttttgta
    39661 taaacaatat atttatttcg ctttttataa gctttgattt tgaatcctaa aaactctgag
    39721 ttagaatatc taacatctgt gattttggat ttttcttggc ttatctctaa tcctaatctt
    39781 tcttttaacc atttttgtgt agctataaaa attcgattag cttcgctaac atttttacac
    39841 ataattttaa aatcatctgc atatctaact atatacattt cttttaagtt agtttttctc
    39901 aatgctctgt ataaatggct tgtagtagag tattgataat gagttttatg tgtttgccat
    39961 tggttgctta tccaccagtc taattcgttt aaaactatat ttgatagtag gggagaaagt
    40021 attccaccct gtgggactcc tttgttagga atcccaactt tgtcgatttc agcttttaac
    40081 attgaactga taatacttag gagtttctta tcgtgtatac cgattgacca aagttgtttt
    40141 aataatttgc tatgcttaac attatcaaag aaacttttaa tgtcaatgtc tataacagta
    40201 tacatatgtt caaagttaat tttcttaact acatatgcta aagcgtgttc cgtaccacga
    40261 ttaggtctaa aaccataact atgtggatga aactttgctt cacatattgg ttctagaact
    40321 tgctttatac attgctgaat tattctatct tctatacagg gtattccaag aggtcttatt
    40381 ttcccattag gttttggaat ttcaactcgt ctaacttttt gaggtttgta attcattaac
    40441 ctgttttgta tatactggac aaatatttta gtatctttcg ttttccagta atttatattg
    40501 tgtctatttg tacctgctgt tttacttcct gtattagttt taacgtttct ataagctagt
    40561 aatatatttt cttcactaga tattaaatta aataatttgg tgaacttact tccttcaagg
    40621 ctttgtttat acaaattgtc gaatgtattt tgcaagttat aatattcatt atatcttagt
    40681 ttgtctttct ttaatggttg taaatcctgt ttaattattg acttcgtatc tgtattatac
    40741 atcgtatagg tcgattatct cctttgtata tatttataag gaatataatc tttcttaatc
    40801 gtactcgaac cctaatgtaa tttgaaataa tagataattt ttagattgtt ggctaaagcc
    40861 catccctcca ccacttatta tcatggcttc aacggtacta tggcttcgct ttcaggagac
    40921 attaacagaa gttatattag atttgtacat attttgatgt tgtataagga ggatagagaa
    40981 ctcatcctcc ctaatatgct ttagcattat atagtgtgta ttctttatat aatgttttgt
    41041 aatatatatt aaaagtaagt ttttgagaat tatgtgtttg tttccttttt tttttagtta
    41101 gggtttagag aaaatattaa atattttctc tatttttttc cctatgtact attacaaaga
    41161 tttaactatt aatataaagg ttaattacct ttatatttgg gattctttat gggttggtta
    41221 gcgaccaacg ggttggagat gaagggtggt tcatctccaa catttcctgc tgaatccttt
    41281 ggagtattta ttgttgggtc atgcctacca atttcttgcc ttattgcttc tatagcttcc
    41341 tgcatctcaa tagcctcttg tttctcaaac ttttcctctt tgtaattttc cactaaatct
    41401 ctaaaaactt tctttattcg tttcatggct ttaaccttcc ttattatatt tttgcataaa
    41461 aaacccatta aagcatttta tacttaagga tagattaaca tatttaccaa aatttgtcaa
    41521 tatattacat caaaatattc taaatttcct tctgaacgct tcattgactt tgtattaggc
    41581 ttatctcagc gttgctccct tcccaagttc caataccttt atcttttacc atatagtttt
    41641 aggtttctcc tctgaccctg acctcttgat aatgccttta acattataaa gtatttcata
    41701 tcaacaaatt ttactctact tcaaggtcta ctgtttaagt cccattacat ttctataatg
    41761 tttgtgttta gagccgtaca ttcggagatt cgtcagtagt ttttacctac tattctaacc
    41821 atgactattt tatagacgcc cagcctatta tgtacttaac tacctctagt tacatttcct
    41881 gtttctctat agattctata agtaaggttc agacctgtag aacctagtca catgacatag
    41941 cgaatcatat agagccttag ggtacactct cagctgtctt tatcgagctt ataaccttca
    42001 attcccaatc aattgaacgc tattcgaagt atcagcgact gcctttcaag acgttactct
    42061 atcattcgac agttcaaggt atcagttcaa taattaataa atcattattg gctaatcttt
    42121 tcaattagca acttgtcgca ctgttgacac aatcccaccc taaaccatat ccaagtatag
    42181 agtcttctcc ttcaggccat agaccattta aatattcttt gttttccata gcttttactg
    42241 aagcaggaga aagaatacca tttgaatttt tcataaatgt ttgtgcaaat gtacatagat
    42301 tttctgcact tgaatataaa ccacctgcac ctatagcatt aaggttatct tgcggaactg
    42361 catcttccca ataaggcacg tatgcttttg ctagttttga actatcaaaa ctattttctg
    42421 tagtttttgt attttgtaag ttaagtggat tatttatgta tttatcaaga aaattagtaa
    42481 atgacatacc agatactctt tctactaaaa tttcagctaa agtaaatcca tcattacaat
    42541 aaacggaaaa agctcctggt ttagctttta atctttgttt ttgtaattct tttaagaaat
    42601 tatcatgacc ataagaatca ttgtctgcta ataaaatagt gtttttaaaa ctacttccca
    42661 tcaaccctga tgaatgattt aataacattc ttggagtgat ttccttgtat ctatcatcag
    42721 ccattttaaa ttcaggtata tactttacaa caggagtatc taaatttaat tttccatcat
    42781 caaccaactt cataacagct gttgtagtaa acattttact gatagatgcg atactataca
    42841 tattatcctt atttaagttt ttgttatcct gtttgctgta aactccacca ttaccagaaa
    42901 tctcaatttt gccattatca atcaaagcat attgagcact tacagcacca tactttgata
    42961 gcaaagtttg gattttctct ttagcaagag ctttagtttt tgtgtaagat tccgcgtttt
    43021 tactataatc atgtaattct atatcagaac tttgatattt ttcggaatta tcttttgtat
    43081 tatcagcaaa agtaatattt ggagcagcag taaataacat actcaatact aatgtactag
    43141 ataataattt agagagcctt tgcttaaaca tattacatta cctctttccc tttttaataa
    43201 tttataatta gcataacatt cttaattatt ttaatcaaca gattattatc aataaaaaaa
    43261 gcttttataa gtatagcttt ttatataaca gagaaaaata taaaaataat atttgttgtt
    43321 gtataaagaa taaagggtat aggtaatata taaataaaat ggaggaattt tagacaatgt
    43381 ataagaatat aaataaaaag caacttaaag aaatgatgaa aaatgaaaag gatgttttat
    43441 tattagatgt aagaactaaa gatgaattta aggaatataa aatagaaaat gctataaata
    43501 tatctttaca agagttaatc aataatatag atgaaattta cgattataaa gataagaaag
    43561 tagtagttta ttgcagaagt ggtcatagaa gtgttactgc atgtaatttg ttagctgaag
    43621 aaggttttga gcatttatat aatttaaatt caggaataat tgattatatg agttaaataa
    43681 tgtaagttat acattaaata ctatgtatag ctctgcttat taagtaggaa tatatctaaa
    43741 tatttataag ataaatttag gaaagaacga attttttcgt ttttttctat gacagttttt
    43801 gtagtaaata ttagttttta agttggaaaa aggtagtagc tttttagcta ctacctttta
    43861 atatgaactt agaatattat taaaaaaaat atatttaaag acagaattaa ttttttaatg
    43921 accagttaaa ttactatcgc cgaatttatc attaaatggt tttataactc tatttatagg
    43981 tccttttaga agtacattta aacaaataga cagaatcata aatagtaata gaactagtat
    44041 atctttagtt agatttggag catatatacc accaactgct tcccttgtag cacctattgc
    44101 ataggtaaat ggcattatag ggtttattct ttggaagaat gtaggtgtaa cctgtatagg
    44161 gaatgtacca ccagaagaac caatctgaac gactaatagt actattgcaa ttgcttttcc
    44221 tacatttcca aatacagaaa ctaaagaata cactataaag ttaaatacca tactagtaaa
    44281 cataagcaga actatcaata aaattggatg ttttgctgtt actcctaaca ataatatatc
    44341 tccagttcct attataaaac cttggacaag agctatactt aaaaaagtaa ggcctcttcc
    44401 aaagtaaatt tcataaggtt tatagtttcc atgtacagat gtagacaata gtgctgaaat
    44461 aagtaatata ccaacccatg tagaaagaac actataaaat ggtgtcattg cagaaccata
    44521 attttcaatt ggatataatt tctctgtttt cacctcaaca ggttctttta agaaattaga
    44581 ctttgtcaat atatcacttt caagcaagct gactaatttt ttcatatctt caccattact
    44641 aattttgctt aaagtatcaa tcaaatcatc caacattcct tttgctaaag gaagtctttc
    44701 tcttatcaat gatatacttt cctgtgcatt tcctgaaagt tttagagaag tagtaagtat
    44761 ttcttcaacc tttggaagtt ttgcttctgc cttatctaaa acagtaatta tatcatttgc
    44821 tacttttata ctgtttgcaa atatactatt tataggtttt gatatttttg agtcaaaatt
    44881 atttaaaata tttaaattaa ttcttccaat accatttgat agacttaaaa cattattcaa
    44941 agcacttata gatggttgtt gtccagatat aactttgttt ttaatatcat ttaatgttga
    45001 tatagatgaa tctattttat tgctagagtc ttcaagatta tctattacat catccaatct
    45061 attatttgat gtaagttggt ttaatttagt taaaaagtcc accaaagtat cgtttaaaga
    45121 ttgcaaatta gaaagttttt ctgataaatt atctatcaac ttaggtacat cttcactgcc
    45181 actatttact gcatcaatta aatttaatgt aagagaagaa gcagaagatg aaaggtcaac
    45241 cattaaattt aagtcagact taataagagg agaaagttcg tctaaattat catttgtatc
    45301 ctctaagaat ttttttaagt cacttgataa tagttttgta tcattcagag tctttttaat
    45361 tgtaggtaaa tcagatttaa tatctttaac tgtatcagat aattttgaag tagcatcaga
    45421 agctaaatca acagtcttat ttatatggtc aaatctcttt tgaaggtcaa taagggaatt
    45481 ttcaactttt gtcaattttg gaagactgtg ttcaattcct gcaccaactc cgtttgatat
    45541 accaagaacg atttcactaa ctgtttttac tatagtctga ttaacctgta gttgtattgt
    45601 agaagcacct ttatctgtaa tctttggtgc aactgcattt attttttcat taacagtata
    45661 gataatttcg ccttttttaa catcatctgt aattaaagat gtcaaatctt ttgtaaaatt
    45721 ttttggaatc ttaagtgatg cataataagt acctttttta acaccttcaa gagcagtttt
    45781 ttcatctaca aactgccaac ctaaactctt gttatctttg agttgttcaa ttaattggtc
    45841 tcctacatta atcttttgtc ctgctatttc atttccttta tcttcatttg ttactgctac
    45901 aagcatatta gcagtgcttc cataagggtc ccaagatgcc tttatattga accaagcata
    45961 cagagatggt aaaaaagcaa gacctaaaac tattacaagt acggcataat tgccacgtat
    46021 atcttttagg tcacttttaa aaatttctaa tatatttttt aaaccctttg aaggtcttaa
    46081 attcttgata ttattccagt ttaaaatttc ttttaaacta ttagtttttt tcaaatgtct
    46141 cacctcctaa aatattttga taaagaaaaa agttagattt ataatcaatt ttaaattata
    46201 ttacttttta cacaaatagt aaagttaaaa ttttgtctt
//
LOCUS       DAYAOY010000024        44577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYAOY010000024 DAYAOY010000000
VERSION     DAYAOY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.024"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..830)
                     /locus_tag="ACGI6B_001413"
     CDS             complement(<1..830)
                     /locus_tag="ACGI6B_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9750865.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYG
                     "
     gene            complement(802..1518)
                     /locus_tag="ACGI6B_001414"
     CDS             complement(802..1518)
                     /locus_tag="ACGI6B_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750866.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(1932..2588)
                     /locus_tag="ACGI6B_001415"
     CDS             complement(1932..2588)
                     /locus_tag="ACGI6B_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9750867.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(2643..3272)
                     /locus_tag="ACGI6B_001416"
     CDS             complement(2643..3272)
                     /locus_tag="ACGI6B_001416"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9750868.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(3403..4044)
                     /locus_tag="ACGI6B_001417"
     CDS             complement(3403..4044)
                     /locus_tag="ACGI6B_001417"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFG9750869.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(4053..4724)
                     /gene="rpe"
                     /locus_tag="ACGI6B_001418"
     CDS             complement(4053..4724)
                     /gene="rpe"
                     /locus_tag="ACGI6B_001418"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9750870.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(4729..5613)
                     /gene="rsgA"
                     /locus_tag="ACGI6B_001419"
     CDS             complement(4729..5613)
                     /gene="rsgA"
                     /locus_tag="ACGI6B_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFG9750871.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(5711..6448)
                     /locus_tag="ACGI6B_001420"
     CDS             complement(5711..6448)
                     /locus_tag="ACGI6B_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFG9750872.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(6556..8571)
                     /gene="prkC"
                     /locus_tag="ACGI6B_001421"
     CDS             complement(6556..8571)
                     /gene="prkC"
                     /locus_tag="ACGI6B_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HFG9750873.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(8588..9340)
                     /locus_tag="ACGI6B_001422"
     CDS             complement(8588..9340)
                     /locus_tag="ACGI6B_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFG9750874.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(9360..10391)
                     /gene="rlmN"
                     /locus_tag="ACGI6B_001423"
     CDS             complement(9360..10391)
                     /gene="rlmN"
                     /locus_tag="ACGI6B_001423"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFG9750875.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(10399..11724)
                     /gene="rsmB"
                     /locus_tag="ACGI6B_001424"
     CDS             complement(10399..11724)
                     /gene="rsmB"
                     /locus_tag="ACGI6B_001424"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFG9750876.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(11786..12487)
                     /locus_tag="ACGI6B_001425"
     CDS             complement(11786..12487)
                     /locus_tag="ACGI6B_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFG9750877.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(12556..13308)
                     /locus_tag="ACGI6B_001426"
     CDS             complement(12556..13308)
                     /locus_tag="ACGI6B_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HFG9750878.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(13318..14247)
                     /gene="fmt"
                     /locus_tag="ACGI6B_001427"
     CDS             complement(13318..14247)
                     /gene="fmt"
                     /locus_tag="ACGI6B_001427"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFG9750879.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(14262..14702)
                     /gene="def"
                     /locus_tag="ACGI6B_001428"
     CDS             complement(14262..14702)
                     /gene="def"
                     /locus_tag="ACGI6B_001428"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFG9750880.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(14720..17209)
                     /gene="priA"
                     /locus_tag="ACGI6B_001429"
     CDS             complement(14720..17209)
                     /gene="priA"
                     /locus_tag="ACGI6B_001429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFG9750881.1"
                     /translation="MKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVIKIGHRILVPFGK
                     GNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECI
                     NLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVD
                     KLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKIN
                     VGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIY
                     KSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGL
                     DSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGAR
                     SALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIE
                     EYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEE
                     NNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKE
                     CPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEA
                     DVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKE
                     GKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLV
                     IKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLK
                     GIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(17484..18683)
                     /gene="coaBC"
                     /locus_tag="ACGI6B_001430"
     CDS             complement(17484..18683)
                     /gene="coaBC"
                     /locus_tag="ACGI6B_001430"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFG9750882.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(18688..18954)
                     /gene="rpoZ"
                     /locus_tag="ACGI6B_001431"
     CDS             complement(18688..18954)
                     /gene="rpoZ"
                     /locus_tag="ACGI6B_001431"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFG9750883.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(18957..19574)
                     /gene="gmk"
                     /locus_tag="ACGI6B_001432"
     CDS             complement(18957..19574)
                     /gene="gmk"
                     /locus_tag="ACGI6B_001432"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFG9750884.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(19604..20485)
                     /locus_tag="ACGI6B_001433"
     CDS             complement(19604..20485)
                     /locus_tag="ACGI6B_001433"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFG9750885.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(20489..21319)
                     /gene="dapF"
                     /locus_tag="ACGI6B_001434"
     CDS             complement(20489..21319)
                     /gene="dapF"
                     /locus_tag="ACGI6B_001434"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFG9750886.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(21606..22640)
                     /gene="ltaE"
                     /locus_tag="ACGI6B_001435"
     CDS             complement(21606..22640)
                     /gene="ltaE"
                     /locus_tag="ACGI6B_001435"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFG9750887.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            22936..24711
                     /locus_tag="ACGI6B_001436"
     CDS             22936..24711
                     /locus_tag="ACGI6B_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HFG9750888.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(24926..26578)
                     /gene="cplR"
                     /locus_tag="ACGI6B_001437"
     CDS             complement(24926..26578)
                     /gene="cplR"
                     /locus_tag="ACGI6B_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Contributes to intrinsic pleuromutilin, lincosamide
                     and streptogramin A resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein CplR"
                     /protein_id="HFG9750889.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(26877..28088)
                     /locus_tag="ACGI6B_001438"
     CDS             complement(26877..28088)
                     /locus_tag="ACGI6B_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9750890.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(28372..28905)
                     /gene="pyrR"
                     /locus_tag="ACGI6B_001439"
     CDS             complement(28372..28905)
                     /gene="pyrR"
                     /locus_tag="ACGI6B_001439"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFG9750891.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(29265..30212)
                     /locus_tag="ACGI6B_001440"
     CDS             complement(29265..30212)
                     /locus_tag="ACGI6B_001440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9750892.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(30181..30627)
                     /gene="lspA"
                     /locus_tag="ACGI6B_001441"
     CDS             complement(30181..30627)
                     /gene="lspA"
                     /locus_tag="ACGI6B_001441"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFG9750893.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(30937..31716)
                     /locus_tag="ACGI6B_001442"
     CDS             complement(30937..31716)
                     /locus_tag="ACGI6B_001442"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9750894.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(31771..32253)
                     /locus_tag="ACGI6B_001443"
     CDS             complement(31771..32253)
                     /locus_tag="ACGI6B_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFG9750895.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(32519..33940)
                     /locus_tag="ACGI6B_001444"
     CDS             complement(32519..33940)
                     /locus_tag="ACGI6B_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFG9750896.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(34086..34265)
                     /locus_tag="ACGI6B_001445"
                     /pseudo
     CDS             complement(34086..34265)
                     /locus_tag="ACGI6B_001445"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(34278..34856)
                     /locus_tag="ACGI6B_001446"
     CDS             complement(34278..34856)
                     /locus_tag="ACGI6B_001446"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750897.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGI"
     gene            complement(34861..36063)
                     /locus_tag="ACGI6B_001447"
     CDS             complement(34861..36063)
                     /locus_tag="ACGI6B_001447"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9750898.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            36929..37675
                     /gene="cdtR"
                     /locus_tag="ACGI6B_001448"
     CDS             36929..37675
                     /gene="cdtR"
                     /locus_tag="ACGI6B_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HFG9750899.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
     gene            38064..38589
                     /locus_tag="ACGI6B_001449"
                     /pseudo
     CDS             38064..38589
                     /locus_tag="ACGI6B_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ia"
     gene            38582..40149
                     /locus_tag="ACGI6B_001450"
                     /pseudo
     CDS             38582..40149
                     /locus_tag="ACGI6B_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(40801..41802)
                     /gene="trpS"
                     /locus_tag="ACGI6B_001451"
     CDS             complement(40801..41802)
                     /gene="trpS"
                     /locus_tag="ACGI6B_001451"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFG9750900.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(41825..42523)
                     /locus_tag="ACGI6B_001452"
     CDS             complement(41825..42523)
                     /locus_tag="ACGI6B_001452"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFG9750901.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(42566..42806)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(42897..44300)
                     /locus_tag="ACGI6B_001453"
     CDS             complement(42897..44300)
                     /locus_tag="ACGI6B_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9750902.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT        14658 a         7843 c         4568 g        17508 t
ORIGIN      
        1 taaccatact ctacactatc acctttactt attactttag gtacagcata tactaatgtt
       61 atatttttta ttggagataa cttatatgaa attgaatttc ctcctaaact tgtatccatt
      121 aaatcttctc ttgctttaga taacaacatt tgattaataa ttttttgatt tactccataa
      181 gaatccacaa tatttacttt tccatcttta tcaaaattca ttacaacgta gaatctatag
      241 ttttctttta tatctgtaat ctgcttttgt ctttcctcat cattcatttc tgtatttttt
      301 gactgatttt catcagaatt ttcttttgaa gcatcatttt ttgttgtatc aagtactcct
      361 aattctttat tatcattaaa aaattcaaaa ttgctttctt tatcaactaa atagtacttt
      421 aaattctttc ctgacgcttt aatgaaatgc tcccaatttt caaagttttc attaaaatct
      481 gcattaatac tatcatctgt atagtaagtt tctgaaccat cataagtatc tttttcttta
      541 cttacatttt ttatatagtt catagtttct tcattgataa atagttttga tggttgtatc
      601 atcttacctt cttgttcttc cttcatatta tgatataaaa cataacttcc tttatatata
      661 ctatctacaa agctttcctg ttcaaatgga ttctctgctc cacctttagc acttgcttgt
      721 atagtagaat attgactaat catacctaat gatgctgata acataagtat aaaaactata
      781 atagtaacta tatatttatc tttaaatttt ttcaattttg tatccaacac cccacaccac
      841 ctttaaatat ttaggatttt ttgggtcaat ttctatcttt tcccttatat tcctcacatg
      901 taccataact gtatctgtat ttacagcatt ctcattccaa actctttcat atatttcatc
      961 agcagaaaaa actcttcctg catttctcat aagtaaactt agtattttaa attctatagg
     1021 agttgttttt atatactttc catcaatact aacttctttt gtattttcat ttaattctaa
     1081 tccgcctatt gcaaacacac tatcgtcact ttctaccttt tgctctttat ttccaaccat
     1141 gtttagatac ttagtgtacc ttctaagttg tgaattaact ctagccaata gttctaatgg
     1201 cttaaaaggt tttgttacat aatcgtctgc tcctatatta agccccatta ttttgtcaac
     1261 ttcctctgat tttgcagata gcattattac tggaaaatta tagttttctc taagcttcat
     1321 tgtaagtgtt ataccatcca ttttaggcat cattatatca actatagcta aatgtatttc
     1381 ttggttttct ataaccttaa gaccttctaa tccattgtat cccttaaaaa cattatatcc
     1441 ttgatttaat agatatattt ctatagcatc tgctatctcc ttttcatctt ctaccaccaa
     1501 aatattatat gaattcattc taatctcctt tatattaatt tataaatttt ctttacatat
     1561 atgattatac aatatgatag aagtttttcc atttatatat atatttatat cttttgttct
     1621 ggttatttgt atttaatgtc catcttagaa acctccttct agtttaaatt taatattttt
     1681 tcattttgtt ttatatactt aatattaaaa ttaaaaactt aagatattac atatactttt
     1741 ctaaagattt tctaaagatt taaatatatt taaaattaat atacaaaaaa agtagttctt
     1801 attgtattaa ttaaatcaat gatttgaaat ataaaattaa tatcaataaa acctactttt
     1861 taatttttta aatatttatt tttaatttat tttgttttta atttacttcg attttaaaat
     1921 gtccttgact tttattttat aaatgtacat atttcttcta ctggtttttt agatggactc
     1981 ttcaaattgt cctctggcac tccaagagct aacattgcac ctaaaaaata ctctcccttt
     2041 tcaaaaccag tttctgcttc taaaacagct tctatctcat ctgctgcata gttttgactt
     2101 gtcaaccaac aagaaccata tccaagttca attgctgata gtgtaaagtt ttcaattgca
     2161 gcacctagac tctgcatacc tggatttctt ataaataatt tatctatagt ctccttaggt
     2221 gcatctataa gctctaattc ataatatcct gatggattat atacctttgt aaaaactaat
     2281 actaaaactg gagcttttaa gtaaaatagt gtaaaatttt taacaaattt tctaaatcta
     2341 ttagccttat ccactgatac tttatccatt tctacaagta tttcttgttg ctttttagtt
     2401 atcacatctg ctatcttttc cattaaatca cggcgtttta taacaacaaa atgccagttt
     2461 tgaatattct tgcctgatgg tgctgcacct gctgctttta tcatttttaa aatatcttca
     2521 tctgatacat cttgattttt aaactttctt acactttgtc ttttaaaaat agtatcttgt
     2581 aattccattt ttaaatcccc ccataacttt tttattattt taatataatt tttaaactca
     2641 tcttatttta atatatcatg ttctaacaaa tctttaaatg aatctataaa ataatcatat
     2701 acatctacat tcccaaaccc atatttggca tatataaaag gtatacctac aaacttagca
     2761 ctatttgcat caccttgcgt atcaccaaca tagattggat ttattaattt atttctttca
     2821 attataagtt tattattttc tcctttatgc aaaccagtat taccaggaca ttcaaaatct
     2881 ataaaatact gttctagttt atgtgctttt aaaaaagatt ctatatatcc agcttgacaa
     2941 ttacttacta taaataattt atatttttta gatagtaatt taagagtatc ttctatatca
     3001 tcaaataatt tagctccatt ttcagataaa tagtcacatt ctttcaaaga acattcattc
     3061 aataaattca ttctttcttc ttcacttaaa tttggaaata aacgatttgc tatttcctca
     3121 aaagaaagcc ccataagact tctaaataaa tcttttgtga cacttaattc tatttcctta
     3181 tgctcactta aaacatcctg ccagactttg catacttctt cagttgaatc ccacaatgtt
     3241 ccatctaaat caaaaataat actatctacc acaaaaaatc ccccttatgt caaagtttat
     3301 tatatattta taattatata ctaaaaagca gatacaatca taaatatatt ttacttgatt
     3361 gtatctgctc tacaatataa aaccaattgt caaaatcaaa ttttataaat ttctaactac
     3421 aagtacacag ccttgctcta ctttaatctt acaagaatta ctcaacataa catttgatat
     3481 tccaataggt acagagtatt tcatataata attatcgaga gggtattcta agccagtaag
     3541 tgttacaccc ttagcatcac catttatagc tattacagaa atagtgtccc ctgggtttcc
     3601 atacaatgaa atttcctcat tttcaactat atacatctct tctttatcag aaagtatcct
     3661 agaatatata ccatcttctt ttaaataata taatagcttt atatttgcaa gagtgtggtc
     3721 aatccttccc cctaaagcac caaaaaaatc aatatgattt gctttaagtg tcttagctaa
     3781 aaaaagacaa agctctgtat cagtctcatc ttttttagat ggaaattttt caaatttaac
     3841 acctttattt ttatagaagt ttatcttttc ttcttctact gaatctaaat ctcctaatat
     3901 ataatctggc attatttcca ttttataagt gtgatttgcc cctccatctg cacatattat
     3961 gcagtcataa cattccttaa ttataatatc tcttgtaact tcgtaatctt caatttcgcc
     4021 atttaaaaca atacagattt tcattattaa atttaaacta attcttcaag agaagcattc
     4081 tttctaaata aattaactgt ttcttgaatg tcatcacttc caaatattgc agaacctgct
     4141 actattacat tagctcctgc ttgtacaact tcggctacgt ttttagggct tataccacca
     4201 tcaacttcta tatcaatgtt taatccttga ctatctataa tagcttttaa ctctttaatc
     4261 ttatctaaaa cacaaggaat aaatgattgt cctccaaatc cagggttaac agacattaat
     4321 aataccatat ctaaatcagg tagtacatgt ttaatcgtat ctactggtgt agctggatta
     4381 agtactacac cagcttttat tccatgtgat tttatatttt gtatagttct atgtagatga
     4441 acacaagcct cctggtgaac agttattata tcacatcctg cttttacaaa ttcttcaata
     4501 tactggtctg gattttctat cataagatga gcatcaaata ccatatttat attttctttc
     4561 tttaaacttt tgactacaag tggtcctaat gtaatgtttg gtacaaagtg tccatccatt
     4621 acatcgatat gaagatattc acatcctgca ctttctactt ttcttacatc ttctaataat
     4681 tttgcaaaat ctgctgataa tattgatggt gctaatttaa tcatgtttct aatacctcct
     4741 actattattt tgtcttattt catgtagcag ctgtatataa ctatcatatc tttctttaga
     4801 tatttctcca tttgtaacag cttcttttat agcacaatta ggttcatttt catgaataca
     4861 ctttgaacca aacttacaat cattaaattt atcaaattct ataaaataat ctttaagttc
     4921 aacttcctct atatcttcaa gggcaaggga actaaaacct ggtgtatctg caaccattcc
     4981 accaaattct aattttaaaa gttcagcgtg acgagtagta tgttttcccc tttttatttt
     5041 atcacttaca actcctgttt gaagtttaaa attttcatct atttcattta ataagcttga
     5101 ttttccaact ccagatggtc ctgcaaacac tactacattt tcttttaatt cttcttttac
     5161 tttatctata tttaacttag taatattgct tacaggaatg actttatagc cactaagttc
     5221 atatatattt ttaacagttt ctaatgtatc attatcatct aaatctgctt ttgtaaggat
     5281 tattacagtt tctaaatttt ctttttctgc caaaactata aatctatcta aaagagataa
     5341 gtttggttta ggatttttta ttgcgaatac tattaatgct ttatctacat ttgcaatagg
     5401 aggtcttata agttctgtat ctctactgtc gatttcttct aagataccct ttttttcatt
     5461 ttcatcaaca atgcttattt tgactctatc accaaccaaa ggtgttattt tctgctttct
     5521 aaatatccct ctagccttac attcataaat accattataa gtatctacat aataaaatcc
     5581 actaatcccc tttataattt ttccttctag catcaaatgc ctccctttat aaaatttata
     5641 taaacacata ctgtttatga gtagtatatc gtgttattct ttcctatttt tatataatac
     5701 cacatttttt ttaaataaca aatcctccgt ttggactaat tacctgacca gtgataaaat
     5761 ttgatttgtc tgatgccaag aaaatagcac aatttgctat atcttctgga gttcctagcc
     5821 tcattagagg ggtttcttcc acaagagcgt ctatatcttc ctcattatat ccagataaca
     5881 tgtcagtatt aatgacacct ggagctatgc tatttactct aatatttgat ggtcctactt
     5941 ctttagctaa tgcctttgtc atacctatta tgcctgcttt tgttatagaa taatgcactt
     6001 cacaagacgc acctgatata ccccagattg aagatatatt aattatattt cctttttttt
     6061 ctgatatcat gtattttaaa gcaacttgtg aacaataaaa gcttcctttt aaattaatat
     6121 tcatcatatt atcccaatct tcatcagtta tatctgtaaa caacttatct tgacttacac
     6181 cagcattgtt tacaagtaca tccaatcctc caaattcttt tatacaataa tctaccatat
     6241 cctctacatc ttctctattt gaaatatcag ccttaaaaag ttttacagaa aaattttttt
     6301 catttaatat agtgtacaac tctttagctt cattttctga cttattaaaa tttactaata
     6361 cattgtaacc ttcttttgca aaagctttag acatagcttt accaatccct ctagctcctc
     6421 ctgtaattaa aactgttttc ttcttcataa atacttttct ccttatatta taaccaattc
     6481 agtattttat acaaaacaaa ctgcccttta aagaaaatat tttctataaa aggacagctc
     6541 ttattttatt aatatttatt ctgttttacc aatgtttgcg ccagttcctg atgaagttcc
     6601 tccattatct cctgagtttg agcctcctga actatctcca ttgtctcctg agtttgaacc
     6661 tcctgaactt cctccattgt ctcctgagtt tgagcctcct gaacttcctc cattatctcc
     6721 tgagtttgag cctcctgagt ttccaccact atttcctgag tttgaacctc ctgagttact
     6781 tccattattg cctgagtttc caccactatt tcctgaattt gagcctcctg aatttccacc
     6841 actgtttcct gagtttgaac caggttctgt tggtgtatta ttctctggtg ttgttggttt
     6901 tttatctggt actgttggag tctgtgtatt tgatttttct gaacctttgc ttacatatag
     6961 gtttacctca tcaccttcct gagctcttga tgagccagca cccggactct gacttaaaac
     7021 tgtaccttct ttatatattg agctgtattc atacttaaca gttcccactt ttaagttatt
     7081 ttcttcaata agttgttttg cattactcaa agtaagtccc actacattag gaacagatgc
     7141 ctgacttgaa cctttactta cgacaacatt tatagtatca ccctttttga catttgtact
     7201 tccaccttca ggtgtctgtg atattataca attttcatca acttcactat tatactcttc
     7261 actttttaca ctcaggtata atccttcttt ttcaacagca ctttgagctt cttctaatgt
     7321 catatttaca agattaggaa cagtcaaaga tttattgcca aaccctccag caaataaaaa
     7381 cttatatgct aagaatactt gagcacacaa tatcagtatc aaaacagccg caattacttt
     7441 taatcttctt ctagattttg ggctttcctg ttttttcttc tttttcttag ttctcttact
     7501 tggagtactc ttaggtctct gattcttttt agctctcatt atctcttcct cttcttcatc
     7561 ttcatcatca tcttcttcat aaaaatcatc atagtaatct tcatcatcat ccaaatcagc
     7621 tacttcaaca ggcttaacca ctttttcagg agctggtttt gcaagtgttg gatttacaac
     7681 tttattaatt tctttttcat caattttctt agttgcaaaa tcatcatatt cttttataaa
     7741 atctaaatca atattttttt ctatatactc tatatcttct atcaattctt cagcagtttg
     7801 atatctatct gcactagatt tttctgtaag cttttttatt gtagttctta cactttgagg
     7861 aatcctaact ttttcttctg atgtaaagtc tatatcatca ttaatgtgtt gaagtgctat
     7921 agaaattgga ctatctcccc taaatggaac ttttcctatc aacatttcat ataaaactat
     7981 acctagagaa tacaggtcag cattattagt tacaaattta cctttagctt gctctggtga
     8041 aaaataatgc actgaaccta ttatactacc aatattagtc attgttgaat ttgaaacagc
     8101 tttggctata ccaaaatcag ccacttttac tactcttcct tcatttgata tgagaatatt
     8161 atgaggtttt atatctctat gtataatccc ctttttgtgc gctgcactaa gtgccatagc
     8221 tatttgtttt gttatatcta atgcagtata ttcatctaat ataccttcat cttgtataat
     8281 ctcttttaga ttcttcccat ctacaaactc cattacaatg tagtgaactt ttccatcttc
     8341 accaacatca taaacattta ctatatttgg atgtgaaaga cttgctactg cttctgcctc
     8401 tcttttaaac tttgttagaa attcatcatc atctacaaat tcaggtctaa gtactttaag
     8461 tgcaacagtc ctgtttaata atctgtcttt agcctcatat acaaaggcca ttcctccatc
     8521 accaatcttc ctgatgattt catatcgatt tcctaaaatt gtatctccca caatatcacc
     8581 gtcctaatta tacttttatc actataaccg aaacattatc tttaccagaa acatcattgg
     8641 ccatacttat caattcttca cttatattac tagttctctc atattcataa tctaatatta
     8701 aatctcttat atcttcatca tcaataaacc cagttaatcc atctgtcgca agaagtatta
     8761 tatcactttt tttgatttct ttcttaaaaa tatctacaac aaccatatca tctgttccaa
     8821 ttgctctagt tattctattc ctctgtggat gtcttcttgc ctcttcttct gtaatgacat
     8881 gggccatcat aagttcttca accacggaat ggtctatagt tattttatca aatttttcat
     8941 ctgttaacaa ataacatctg ctatctccaa catttgccac atataagaca ttattataca
     9001 ctatagccgt aactaatgta gttcccattc cttcaaattc tatatcttcc atagattttt
     9061 tgtgcacaat tgagtttaca ttattataag cctgttttaa tatatcatct atatagtcta
     9121 tttttacatt atcatgttgt aataagtttt cttttagaaa atcaattata ttttctactg
     9181 ctaatttact ggcgacttca ccttttttat gtccacccat tccatcagct atggcaaata
     9241 ttcctattgg accatgttct gtatctataa cttctccctc acaatagtct tcattgtttt
     9301 ttcttatttt tcctatatgg gaggcacaac tataaaccat acgacttcct ccttacaaat
     9361 tacttgtatt ttcttctcaa ttgcccacaa gctccactaa tatcagaacc catagacatc
     9421 ctaactgtgg caggtatatt gttcttctcc aaactatccc taaatttata aataaatgct
     9481 ttatctggtt tttcgtattc tctttcttca actttattta ttggaattaa atttacatga
     9541 catagcatac cttttaggag cttagctaat gcctttgctt ctttttctga gtcatttact
     9601 ccttttataa gagaatattc aaatgtaacc cttctatttg tcttttttat ataatatcta
     9661 catgcattca atatttcttc aatagaataa gcatttgcta caggcataat ttttcttcgt
     9721 tcttcatcat atggtgaatg aagtgataaa gctaaattta tagctatttc taagtctgcc
     9781 aactcataca ttttaggtac tattccacaa gttgaaagag ttatatgtct ataccctata
     9841 ttaagaccat ttttttcgtt aattattttt aaaaattgtt ttgtattttc aaagttatca
     9901 agtggctcac cactccccat caatacaaga tttgataccc tcttaccagt atcttcttga
     9961 attttaataa cttggtctaa aatttcccat ggctctaagt ttctaattaa tccatccatt
    10021 gtagatgcac aaaaattaca tcccatccta caacctactt ggttagaaac acagacagta
    10081 actctactat cataatccat catcacagtt tctattatat ttccatcatc taataaaaac
    10141 aaatattttt ttgtattatc taccttagat tctaacttta attctatatc tatatgccct
    10201 atacatgaaa cctcttctaa tttatttctc aaactttttg gtatattgtt catatcatca
    10261 aaagtttttg cacccttata tatccaagag aatatttgac ttcctctaaa gggcttttca
    10321 tctatagttt tcataaactc tttaagttca tcttcagtaa aattttttaa tactatcttt
    10381 ttcttttcca ttctctcact accttattct ctttaatttt gctatgaaaa aaccatctat
    10441 accatgaata ttaggatata tctttaaata tcctttatct tgattgtcta agtctacatt
    10501 tactgtatca attggaacaa attcaaagtt gtgattttct tcaacaaaag aagtaactat
    10561 atttatattc tccacatctt gaattgtaca tgtactatat actaaagttc caccaacttt
    10621 gacatattta gatgcatttt ctaaaatttt cttttgtata gaagtgatgt cttttaactc
    10681 ttcttctttc ttatacttaa tttctggttt acgtcttatt ataccaaaac cagagcaagg
    10741 aacatctgct aaaacataat caaatttact tatactattt tcatcaatct cagaagcatc
    10801 aaatctttct acaaacacat ttttaagccc caatctattt acagtagagt ttataagctt
    10861 tagtttatgc tcaaaaatat ctctagctac tacttgacca gtattgttca ttaaagtagc
    10921 taagtgagta gatttccctc caggagcact acatacatcc aaaacaaatg aaccttcttt
    10981 tggattaatt actttcccta ctatcataga acttatatct tgaattgtaa ataaaccagc
    11041 cttaaataat tcattttttt ctatattttt cattttttcg actttaatag cttcttctac
    11101 cataggaact tttaaacata caatacccat ttcacttaat ttttcaataa gttcttctct
    11161 acttatttta agtgtattcg ttcttatata gatactaggc ttttcattat ttgcttccaa
    11221 caaatcttca gcaaattctt gtccaaattt atctatccag ttttttataa tccaagagtt
    11281 ataggaatac tttatagata agtattttac actatcttcc tcagttatgt ccattatagt
    11341 ctctttagct cttatttcat ttctaagaat agcatttatg aaaccagaag attttttatc
    11401 atatttctta gataatttta cagtttcatt aactgctgca taatctgata tactatctaa
    11461 aaataaaatc tgatatgttc ccattcttaa aaagatttta acataagttg acattttttt
    11521 tacctttatt tttgaaagtt tattgattat ataatctaaa taatatttat tttctattac
    11581 tccataaatt aactctgttg caagtcctct atccatatca cttatttcta aatttttaaa
    11641 atgcttattt attgcaatat ttgaataatt gtcatttttt tctatatcac ataatacttt
    11701 aaatccaact tctcttgcat ccatataaat ttctcccttt tattaaacat aaaaaagggt
    11761 tgtttctaaa caacccactt tacttttagt catttcttct ttgagctatt agtaacaatc
    11821 taagtaattg aagtattgaa accaatgctg ctgcaacata tgttaaagct gctgctgaaa
    11881 gtactcttct actttgtctt gactcacttt catctataat ccctaaattc ccaagttgaa
    11941 cgattgctct acttgaagca ttaaattcta caggcaatgt tattatttgg aataacactg
    12001 aagccgaaaa caatagtata ccaattttta aaaatggacc agccataata aatcctaaaa
    12061 atattaaaaa ccaagatata cttgaagcaa aattcactac tggtactaaa ctacttctta
    12121 tttgaagagg tgcataacct ttagcatgtt gtatggcatg accacactca tgtgcagcta
    12181 ctgcaacaga agttatagat gttccataat atacatcttc tgacaacctt acagcctttc
    12241 ttcttgggtc ataatggtca cttagatgcc ctctaaccat ttctatcttt acatcataca
    12301 aaccatttga atcaagtact cttcttgctg tttgctctcc agtatatcct cgacgtgtat
    12361 ttactcttaa atacttattt gttgttgaac tcactttaaa ttgtgcatat atagtaagta
    12421 atatagctgg aattaataca accattgttg ggtcaaatcc ccaaaaaccg ccatatacag
    12481 ggtacataaa taacactctc ctaaaatata tttttattat acatcctctt aatattcact
    12541 tcacttaaaa acagtttaat tacaaactaa tatatttttt cctgttaaaa agcctatagc
    12601 tttttcaata tccataatgt ctacattagt atttacacaa ggtccattag gtcttttgtt
    12661 aaatacacct aaaacaggaa ttttcttaat atcttgtatt ccagatgtca aatctctttc
    12721 acatgcaaca gctacaacag ctttaggttt attatccatt ataatttttc tagctagggt
    12781 tcctccagta gctacaaata tttttacatt agttttttcc tttagcttaa ttaattctcc
    12841 tatgttgcat cttccacatt ctttacaatt atctatatca ttagtaactt ttaatttaca
    12901 actattctct tgaatacaat gaggtattaa aattattata tcctctggat taaaattata
    12961 tttattactg tatatatatt cattatttaa ctttatatat attcttctaa tatcactttt
    13021 tgacatacca aatgatgatg caatgaaaga tataatagga aataatccac tgacaatatt
    13081 aaaatttatt ttcattatat ttgagttaac atactttcct ttaattactc tataagtaac
    13141 tgctgttgaa catagtatta taagtattag tgatattatt ataatattta tcatcaagga
    13201 gaatatttga gttaaactta ttataaaaaa attaacaact cctatcaaaa ttcctaatat
    13261 taaaatcaaa atcccaacac ttgttaaata cttctttata tctgtcatac ttttcaccta
    13321 acccaatact aaaccagttt ctatagtatt cccttttata tattcaccaa ccaacattct
    13381 ctttttattc ggcatctgaa tttcttcaat taaaagtaca ttatctatag tacttacttt
    13441 tatcccatct ttgcttacat caattattgt acctacatct tttgtgcttt tttcatctaa
    13501 cactttagtt ttccaaactt tcattatgac atcattatag gtagtatagg cacttggcca
    13561 tggatttact cctctaacta aattgtgtat ttctctggca ctttttgaaa aatctatgtt
    13621 acctaatgat ttattcatta ttggcgcata ggaaaattct tcatgatttt gtacttctct
    13681 tggagcattt ccttcttcaa ttaatcttaa tgtttctttt aaagtttctg cgcctatatt
    13741 catcatttta tcatgaagtt ctcctgctgt tatattttca tcaagattta cttctgtctt
    13801 tagtatcata tctccagtat ctagcccttc atccatatac atagttgtaa caccagtctt
    13861 ttcttcacca tttattatta cccaatttat aggtgccgca cctctatatt ttggaagtaa
    13921 agaaacatga acatttatac atccaagttt aggaatctct aatatttctt ttggaagtat
    13981 ctgtccaaaa gctacaacta ctattacatc tggatttaaa gatttaattt tatctataaa
    14041 ttctttatct ctagccttta ctggttgata tacaggtata ttattttcaa ttgccagttc
    14101 ttttactgga ctcataccta gttttttacc tcttcctttt ggtttatctg gttgagttac
    14161 aacacccaat atttcatact tctcatctat tattttttgt aaacatggaa ctgctatatc
    14221 aggagttccc ataaatacta ttttcatcaa atcaactcct actacttttc tattttgtca
    14281 acaaacaaaa ttccatctaa gtggtctatc tcatgacaaa atgctctagc taaaagttct
    14341 tctccctcta actctattgt ttcaccattt ctatttaaag ctctgacctt tacataattt
    14401 ggtcttctaa catttcccgc ttctccaaca acacttaaac atccttcctc atctatttgc
    14461 tccccagatg tttctattat ttcaggattt ataagttcta taagctcttc tccaatatct
    14521 attactacta ctctttttaa tattccaact tgaggtgcag ccagtccaac accatctgca
    14581 tcatacatag tttctgccat atcatctaat aattgtatta tcttttcatc aattttttca
    14641 accttttttg atttttttct taatactggt tctcctattt gaactatttg tcttaaagcc
    14701 attataattc ctcctaaatt tataatacac tgtttggatt aatatctata gatacattga
    14761 tattcttatc aaatatcaaa tctcgttttg ttatacatat atattttatt atacccttta
    14821 agagattaat ttcaatattt tcatctttaa agagtatttg ccatctataa ttttgattta
    14881 tttttgatat tgaacatgga tttggaccta atataaatcc taaatcattt ataccccttt
    14941 tttctaataa ataaatcaat gatgcatgca tattttttat attttttatc actagtcttt
    15001 catcttcacc actaacaact acacttaaca tattattaaa aggagaatat ccaaatgctt
    15061 ttcttatttt tatctcatct tcataaaaac cctcataatc atattcaata gcatgcttaa
    15121 tagcatagtg ttcagtgtca taagtctgaa gtactacttt tccctcttta tcagctctcc
    15181 ctgctcttcc agagacttga gttatcaatt gaaatgtagt ttcaaaactt ttaaagtctg
    15241 gaaaatttaa tatcatgtct gcagacaaaa tacctactaa agttacattc tcaaaatcca
    15301 gccctttact taacatctgt gttccaataa gtacatctgc ttctttatct ttaaatttat
    15361 ttagtatctc atctaaagca cctttttttg aagttgtatc tttatccatt ctcaaagttt
    15421 ttatatctgg aaaaatatat tttaattcct cttcaatttt ttgagttcca actccaaatg
    15481 gcttaacata agtactttta cattcaggac actcttttgg aatttctttc tcatatccac
    15541 aatagtggca tcttccagta ttgcttttct tatgataagt taaagatata tcacaatttt
    15601 cacattgaaa cacatatcca cactttctac aagatacaaa atttgcataa cctcttctgt
    15661 ttaaaaatag tattacttga ttattttctt caatcgcata acgtatttct ttttgtaatt
    15721 tcaaactaaa tatacttcta tttcctttat ctaactcatc tttcatgtct accacttcaa
    15781 tgtttggaag aggcttatca tttgctctac tttttatatt tataagtttg tattctccag
    15841 attttgccct ataatactct tcaattgagg gcgttgctga acctaaaata agagttatat
    15901 tattttttaa tgccataaat ctagcaactt ctatagcatt aaattttgga ttcttctcag
    15961 acttatatga tgactcatga cattcatcaa ttattattaa gcctaaggaa ttaaaaggtg
    16021 caaataaagc tgacctagct ccaattaaca ctcttacttt acctgctttg acagctttgt
    16081 aaacatcatg tttttctcct tcagatagtt tactgtggaa cactccaacc aaatctccaa
    16141 acctactctt tagcctatct attgtttgag gagttaatgc tatctcaggt actaagaata
    16201 tactgtctaa gccttgattt agagcaaatt ctattatttc catgtaaact tcagtttttc
    16261 cacttccagt tactccatga attaagtatg gttttttatc atctacaaac atattcgact
    16321 ttatctcatc aactgcttct tgttgctcat tgtttaattt tatacttttt agtacggatt
    16381 tgtaaatact ttttggctct ctataataat ccttaaattc tagggttatt agctttttct
    16441 tacttagaga atttatactt tgttttgaag catttaataa atccaataaa tcatttatct
    16501 ctacattttc attattttta agaaaactta atatctcttt ttgtttactt cccacattta
    16561 ttttattttg ctctatataa tcgtcaatct tatcactttc taaactcaaa gatatataac
    16621 ataccttctt ttcatttttg tgatttttat attcccaaca taattttata attccattct
    16681 tattcattgt gtacaaagaa ctatttaagt ttggtatata ttttaactta tcaaccttaa
    16741 ttttaccttt actatcaatc actttctcta ctatttctct atttttatca cttaaagttg
    16801 atattttttc cttcaattca atatcattta atccacttaa actttcacct aataggacta
    16861 ctttataatt atttaatttg tatccctttg gatatatcaa atttatacat tctatataag
    16921 tacacaaata tctatttttc atccaataaa ccaactctaa atcttctttt ctaaatatcg
    16981 gattttcatc taatatatca ataatttctt ttatttttat tttttcatct aagctatctg
    17041 taaattgaaa tacaaaggct tctgttggtt tattaccttt tccaaatggt actaatattc
    17101 tgtgcccaat ttttattaca tcagataaaa atacaggaat ttgatatgta aataaattat
    17161 ctgtatatat agtattactt ctaactatta cctttgcata ttttttcatt ataaaacctc
    17221 tacttaaatt taatatattg ttcatatttt aatatacttt acaagatatt aaaatctcag
    17281 aatttataag caatttcaag ttattaatat taactttatt ttaataatta actattaatt
    17341 atattttata ctttatgaca aaataaatgc tgaaaactac aatttaactg acatttgcga
    17401 gtttttactc gctaaaatta aattgcagct ttcagccact ttagctgtga taaaatacac
    17461 aaatatcata tttaaaaaag gtcttatttc tcaagcaacg ctttaacttt atctaaaata
    17521 acattggcta cttcttcttt tttcatctta ggatactcag taatatttcc ctctacatct
    17581 attattttta caatattagt atcagctcta aaaccagcac cttctttagt taaatcattt
    17641 gccacaataa aatctaaatt tttctttttt atttttcctt tggcatgttc tataaggtca
    17701 ttagtttcag ctgcaaatcc aattagtatc ttatcttctt ttatttttcc tatttcataa
    17761 gcaatatcat aatttctact tagttctatt gataaatcat cattcttctt tttaattttt
    17821 ttgtcagaat aagtttttgg tttataatct gcaactgctg cactttgaat tactatttta
    17881 ttttcactta aatttgatac tactgcttca tacaaatctt gtgctgactc tatttgaaca
    17941 aattttttaa gattttgtgg aatagaaaga tttgtaggac ctgatattaa tgttacatca
    18001 gcacctctag ctatagcttc tcttgctata gaatatccca ttttaccaga agacctatta
    18061 gttaaatatc ttactgggtc tatactctct acagttggac cagctgtaac tatcatactt
    18121 tgacctttta aatcttgctc tttagtaaga ttagtcaaaa cttcctctac tattatatct
    18181 ggattagcta gtttacctgt tccaatatct ccacatgcaa gccttccact ttccggttca
    18241 ataaatttat atccaaagct ctttaaagtt tgtatatttt tttgaacaat tggattttca
    18301 tacatattag tattcatagc tggtgctata agaacctgtg cttttgttgc cataacagta
    18361 gttgtcaaca tatcgtcagc tataccattt gcaatcttgc ctataacatt tgcacttgct
    18421 ggcactatta agaataaatc tgcttttttt gctaatgata tatgttcaac ttcccaagtt
    18481 tttggttctt caaacatatc ttctactaca taattttgac tcagcgattg aaatgtaagt
    18541 ggtttaacaa attctgttgc tgattttgtc attataacat ggatatttgc atttagtttt
    18601 tttaacttac ttacaacatc acaagcctta tatgcagcta taccgccact tatgccaatt
    18661 acaacagttt tatcttttag catgacctta ctcctcttct gaaatttgtt ggtgttgctc
    18721 agcatgatgt ctagcttctt ctatctctat ttcctcttct gtcaataatc tatatgttat
    18781 ttttccactt gcaacttctt tagttgctac acatacaggc ttttccttag ttttatttga
    18841 aacatatggt tcttctccat caattaattt tcttgctctc tttgaaacag tccctactaa
    18901 atagtatctg ttgtctattt tttctagtac ctcattgata gatggtttta acataattat
    18961 aattcctcct taaatttctc tattataata tttttatatc tgctaacttt attcttttca
    19021 gatgatatta taccttctat ttctttagca gatgttttta catcttcatt aaatataaaa
    19081 taatcataat ccttaatcat tttaatttct tcatatgcac agctaaatct cttctctatt
    19141 tcagcttctg tttctgtacc tcttccaaca attctatttt ttaattcttt aagtgatggt
    19201 ggtagtacaa atataaatac tccctctggg caaacttctt ttatttgttt tgctccttgc
    19261 atttcaatct ccagtaatac atcttgtcct ttttctaaac attccattat tgccgctttt
    19321 ggagttccat aaaaattatc ataaatttga gcatactcca aaaactcacc tttttctatc
    19381 atctcttcaa acttttcctt acttatgaag aaataattta ctccatcaac ttctccagtt
    19441 ctgggttttc ttgtagtagc tgatacagaa agttttattg tatcattttc tttaagtagt
    19501 tctttgcaaa tagtaccttt tcctgcacct gatggtcctg atacaactag caataatcct
    19561 tttttcacaa acatttaagc attccccttc taatattgta ttcttactct atattttgta
    19621 tttgttctct tattttttcc aattcgcttt ttacttctac tactaaatta gttatattta
    19681 tatcagatga cttcgaacct atagtgttag tttctctatt catctcttgt attaagaaat
    19741 ctatttttct acctatagag tcgtctttaa atatagtatt tttaagttgc cctatatgac
    19801 ttttaaatct aactatttct tctgttatac tgcttttatc tgcatatatt gcaacttctt
    19861 gtgctaatct attctcatct attatacttg ggtcatccag taattcactt attctcaaat
    19921 tcaatttttc tctataatct tctacaacac tacttgaata tttttcaatc tcttctatat
    19981 gattttttaa taaatcacat ctcatggcaa tatcttcagc taatttttta ccttcttcac
    20041 ttctcatttc tcttaatttg attaatgcat cttcaactgc ttgattcaac attgaccaca
    20101 ataaatcttc atcttcttcc ttttcttcta ttttcacaat atcaggaaac tttgcaacat
    20161 tcataacgct aatatcatct ataatatcaa atttattttt tatttctttt agtatgtcta
    20221 tatactgaga tgctaatccc tcatcaaaat ttaagtttac atcttctttg cctaataagt
    20281 caaactttat gtataaatct actcttcctc tttttacata attttttact aaatttctaa
    20341 ctttatcctc taaaaatgaa atttttcttg gaagacgtat gtttatatct gaatacttat
    20401 gatttatcgt tttacactct actaaaaagt aataattatc atccttgtat tctcctcttc
    20461 caaaaccagt catacttata gccattaatt aaacctccaa aataccttca catattttta
    20521 ctgctggacc tgtcatataa gagaaatctc ctacatctat tttaactgtt ccaccttgag
    20581 acgttacatt tacactactt tctaccatat taagtaaatt gcacactatt gctgatgcag
    20641 tcataccagt accacaacct aaagtatatc cacaacctct ttcccaagta taaacatgta
    20701 tattatactt atcttctacc tttacaaaat ttacattcgt tttttcagga aatattttat
    20761 tattttcaat ctcttttcca tatttacaaa catcatctac atctaattca tctactataa
    20821 ctactgtatg aggaaccccc atcaacatag aagtaacttt aagctcccta tctaaaacct
    20881 ttatactttc ttgcacaaaa cgttctttat ttgtaagtac aggaacgtcc ttagctataa
    20941 aacttccttt tcccatattg actcttatag agtttatttc tctattagta agatttactt
    21001 ttatatcttt tataccatct aatgtatcta ctgtaaaact ttctttttta actatacaat
    21061 tatcataaac aaatttacaa aaacatctaa ggccatttcc acacatagct gctcttgaac
    21121 catcagaatt ataatatacc atttctacat ctgcaacttt tgaatctttc acaactaaca
    21181 atccatccgc tcctacagaa aatcttctat ggcatacttt ttttgctaaa gcagtataat
    21241 cagatggatt tatctggtcg aatcttccat ctattgctat aaaatcattt cctattccat
    21301 gtagtttcca aaatttcata agtatcgctc cttcgtaaat taacacttaa actattataa
    21361 cataaaaaaa ttcccataaa cttataattt atgggaaaag ttttgtaatg acaactataa
    21421 tttgaggatg tgagggagag ttgccattac aattagggta attatattat gtttttaagc
    21481 tagttctttc tatgtttata tattaatatt tctatatgaa ttttatgtat gttttattta
    21541 aactgtctat gaatttttta attaaacatc tggtaactag ctataatcct tctagtagat
    21601 atctattaac atacacctat taattcttta agtgttttta aaacatagtc taaatcttcc
    21661 ttagtaatat ctacatgagt tacaaatcta tattcaccat tttccatttt attaattttt
    21721 atatttcttt caaaaaattc ttttattaaa acatcctctt ttataatatc ttcactcatc
    21781 ttaaaaaata ccatacttat atctggatta acattgttta cttcaatgcc atcaatttta
    21841 tttaattcac aagctaaata ttttgcattt tcatggtctt cattcaatct taaagtcatt
    21901 ttttcaatgg caattatacc agctgctgca actataccta cttgtctcat tcctccaccc
    21961 ataacttttt gctttcttct agcttttgct ataaattctt tatttccagc aagtatagaa
    22021 cccattggtg cagctaaacc ttttgacaca cagaacataa cactatcaca acactttgct
    22081 atttctttag cttctacacc caaagttaat gctgcgttaa ataatcttgc tccatctaaa
    22141 tgtactggta agttatgttt ctcagctatt tttttaattt ctcttaaatt atcaagactc
    22201 acaactgttc cacctgaata tgcattttct acacatataa gccctgtctg tggttcatgt
    22261 atgtcatctc ttctaattgc cttttcaaca agttcaggat ttagagctcc actttcgtct
    22321 tcatctatag ttctaagctg aactccagct attacagcag gtgccccaac ctcatgagtc
    22381 acaatgtgat aacctttacc aataattatt tcttgccctc ttaggcaatg agtaaataga
    22441 gctaattgat tcccaaaagt tccacttgga aggaaaatag catcttcttt tccaacttta
    22501 gctgctgcta attcttcaag tttatttaca gtagggtcat ctctaaagat tgaatctcca
    22561 acaggagcct ttgccatagc ttctctcatt tcgtcagtag gcatagtaac agtatcactt
    22621 cttaaatcga taaatctcat tttttcaacc ttctttcata tttatatact atagaacctt
    22681 aaaattgatt ttttctgtaa agttcatata ctttcatagc ttatactcta cacaataatt
    22741 atactatatt tactttatat ttaatctact ttaaattatt aattttaaaa ataaagtttc
    22801 ctctctaata aatataaata attatacaat ttaaatatta atctctattg tttaaattcc
    22861 ccctagtcac aaagcgtata ttatgtgtat aattaattta ctgactcatt ttggtcaaag
    22921 aaaggggtgc tataaatggc attagatgga ttagttatac attcaatagt cgatgaatta
    22981 tcatcaaaac ttacaggtgg aaaaatagat aagatacatc aacctgaaga tgatgaggta
    23041 atttttaata taagaaataa taaagaaaat tttagattgg ttttaagtgc aagtgcctca
    23101 aatcctagag tttatcttac aagtaattat caaaaagaaa accctctaaa agcgcctatg
    23161 ttttgtatgt tacttagaaa atacatacaa ggtggaaata tagtagaaat ttctcaaata
    23221 ggttttgaaa gaataataaa aataagtgtt gaatctttag atgaattaaa agaaaagaca
    23281 gttaaaaata ttatgattga aattatggga agacatagca acataataat aactcatggt
    23341 gaagaaaata aaattataga ttctataaaa agagtccctt ttagcataag tagagttcgt
    23401 caagttttac ctggtcatga ttattcattg ccaccagaac aaaacaaact caaccctctt
    23461 gatgatatat ctaaggattt gtttattaaa aacctagagg aattggaagg acctatcttt
    23521 aagtctatat attctaaatt tctaggtata agccctatta tagctaaaga aatttgctac
    23581 agagcaggtg taaatcaaaa tgctatcata aaggatatat ctgatgaaca atttgattct
    23641 ttacacaaag tattttgtaa tttattcaat gatataaatt ctaacaaata ctctccttgc
    23701 attattatag ataaaaaggt ggataaggta gttgatttca gttgtataaa ccttacatta
    23761 tttagcgatt tatcatatat aaataaggat agtatgtctc gtattctgga agatttctat
    23821 agaactaagg atattaaaga tagaattaat caacgttcat ctgatttaaa gaagagtata
    23881 tctgttaagc ttgatagact ttataataaa cttaaaaaac aagaagaaga attatcagaa
    23941 tccgaaaatg cagacattta taagataaaa ggagaattga ttacttcata tatttatatg
    24001 gtagaaaaag gtatggaaag tattgaagtt gctaacttct atgatgaaaa ttgtaatgat
    24061 gtaacaatag aattaaataa aaacttgaca ccatctgaaa atgctcaaaa atactttaaa
    24121 aagtataaca aaatgaaaca tgctaaagtc gagatatctc atcaaatttc cttaaataag
    24181 gaagaaatag attacttaga aaacataatt ttaagtattg aaaactgtga aaatttagct
    24241 gaattacaag atataaaaga agaacttgct aaggttggat acatcaagac tcaaaagaaa
    24301 aacagtaaaa aggatactat cccatctaca aaacctcatg aatttgtttc ttctgatgga
    24361 tttaaaatat tggttggtaa aaacaataag caaaatgact atcttacact aagacttgct
    24421 gataatgatg acttatggat gcatacaaag aatattcctg gttctcatgt aataataaag
    24481 tgtgctggta aggaagtccc tgataatact gtatttgaag gagctatgct agcagcattc
    24541 tttagtaagt caaaactttc ttctcaggtg cctgtagact atactaagag aaaaaatgtg
    24601 aaaaaaccta gtggttctaa accaggtatg gttatttatg aaactaacag tactatttat
    24661 gttactcctg aagaagaaac agtagccaag cttaaggtta aatctgaata aattctgcac
    24721 taaatacaaa ataaatgtta gacaactaca cattatcatt ctaaataaaa atagtggcct
    24781 tcttataaag tacaataata aggccacttt ctaattagct tacttttaat tttctaacaa
    24841 aatacttttc tgttttaata actatactta tattcccaac aaatattttc agtcttctta
    24901 tatagatgta attaaaaagt taacactaaa agctattttt taatttattg atttgtttta
    24961 gtaattcatt atattctgat tcatacaatt ctttttttag attatctttt tctatagata
    25021 acatagatat aacagttgta agtctatttt ctaatactag aagattttct tttttgattt
    25081 ggttttctct actaggagtt tttttatttc tgctaattgt atattcgtca taattacaat
    25141 caaactccct tattttagca tcttttatct ctataatata gttacaaata tgtgatacaa
    25201 atactctatc atgagacact ataagcatag ttttatcagt atttatcagt gccttttcca
    25261 gtgcttccat agattttata tctaagtaat ttgttggttc atcaaatact aaaaaattat
    25321 tatcttccaa tattatttta catagtgcta tttttacctt ttctccacca ctaagtactt
    25381 ttactttttt gtagacatca tcacctttaa acccaaatag gcttaagttt atccttataa
    25441 aagattcatc aaatgaacta ttaactttag tattgtatag aacactcttt tcctcatcta
    25501 ataaactttg attttggtca aaataaccta cttttacctt gttatttatt tttatattgt
    25561 catttttatc agccaatatt tcttttatca gagtactttt tccacaccca ttatcaccta
    25621 ataatgctat ctttttacct ctttttatct taaaggaaac attatctaac aggagcttat
    25681 tttctaactt tagagtcata tcttttacct ctactagatt tttactaatt atctccatac
    25741 catcttgaat cttaatgttc atttctttag aaactttagg tttattcttt acctcaagtt
    25801 tatcaattct gctttttata gccttcacat ttgagtccaa ctttttctta ttagtctgac
    25861 cacccatttt gtgcaaccta gcttctgaat tacccattct ttttggtgtt tttcttatgc
    25921 catcacttaa agctttttta tataaaagag ctttttcaag cctttttttc tctttaacat
    25981 attcttgata ctcaaaatca gctcttttta cttcttcatc tttttgcatt aaataatcag
    26041 tgtaattgcc tttgtaagct tttagttttc catcttctag ttctaatata gttgtacata
    26101 actcatccag aaaacgtcta tcatgagata ttaataataa tgctccctca tgccttttaa
    26161 acatatcttc caaaacccca atgctttttt tatctaagtt tgaagttggc tcatcagcta
    26221 ttatgatatt tttcttatta ctcagcgcat ctgctatttt taacttaacc ttttcccctc
    26281 ctgataagtg ttcttcaaat ttatctggag catttaaaag actcttctcc ttactatgac
    26341 tacatgtttc aatattattt tcactctgac ttatataaga atagctttct gtaaggtaag
    26401 tatatccttc atcaatttct atttctccca atattgcttt taataatgtt gtttttcctt
    26461 gaccatttga accaactaga cctattttgt cattttctaa tatttcaagt ttatctatat
    26521 ccaaaattaa tttatctgca taatatttct ttaaattctc tacttttact aacaacataa
    26581 aaaatccctc cttaacacac ttaaatataa ggagagattc tataaatagg cataacaaat
    26641 aaacgtatag actataatct actctcctta atttaagcat tgcaaataaa gccaccaaaa
    26701 taggcttttc aaaaattaca atactttaaa ttataaaaaa tagattatat tctcattgtc
    26761 ctagttacct tgccctttct ttcatttatt tttaatcatt atatcaacat taattacaaa
    26821 tgtctataga aaattaacta attttagtta ttcaatatac aacttattct ttaaatttat
    26881 tcttcttcaa ctttaaagtc ctgattattt aaaattctgt ttaatacaat tccaacaatt
    26941 gcagctaaac taagaccagt tatctttact gtacttgtta tagggattcc aacagaaata
    27001 cctttattag ataaatatgt tgttccaaga cctattaata atataactgc tatgattaat
    27061 atatttattt tactttctac acaagaactg tcccttatag tttttaatcc tacataagtt
    27121 atcatcgaaa atagcattat actaattcca cccataactg aacctggtat actttgaaga
    27181 aatccaccaa acttgcctac acatgcaagc aatatagcaa atattgctgt acgtctaagt
    27241 attgaagggt catagttttt agttatagca agtactgctg tattttctcc ataagttgta
    27301 tttgctggtc ctcctaaaca ccctgcaact atagtcgcaa atccatcccc aagtaatgtt
    27361 ctatttaatc caggattttc tataaagttt ttacctacta cagcaccatt agtagtcata
    27421 tctcctatat gttccataaa tacagctaat acaactggag atataatcac tattgctcct
    27481 aaactgaact taggaactgt aaagttaggt attgcaaata aacttgcttc tgtaatagta
    27541 gttatatcta ctaatcttaa tatcaaacag attatatacc cagaaaaaac tgctattaat
    27601 attcctaatt gttttataaa tcctctacca aacttattgg ttaaaattgc tattgctaaa
    27661 gttataaatg ctatcacaaa attggctgaa gccatactaa aagctgttgg caataaattt
    27721 aatcctataa ctactatcat agctcctgta acttgtgcag gaaaatataa tttaatttta
    27781 tttacaccaa ctacttttac tataatcgac attataatat aaattaatcc tgctactatt
    27841 ataccacctt gagcatatgc taaatctcca ccatacgcct ctttagctgc tagtataact
    27901 ggtataaacg caaaacttga acctaaaaag gcaggtactt ttccttctgt acaaaaatga
    27961 aatatcaatg taccaacacc agcacagaaa attgcaactg acggattaaa acctgtcaaa
    28021 atgggaacta atactgtcgc tccaaacatg gctaatagat gttgtaaaga cattatcgtt
    28081 tttttcataa aaacctccaa aattattata tataaataaa tcctctgttt aattaatacc
    28141 ttttaattaa ttatcattcc ctagattcta ttttttaacc tctatatact tattatataa
    28201 tttatcatta ttttacattt tacgacatca aaaactcaaa taaaggagaa taaaaactac
    28261 attaagactc acatccataa aattataata tttattttgt gcaatctatg taagctttat
    28321 cctcctccct gactattcaa tgctaagctt ttatataata cttaaaaata attattcttt
    28381 gattgtaact gaatcttctc catcaatttc taataaactg acagatataa tttcatgtct
    28441 agaagtaggt acattttttc ctacataatc tgctcttata ggcaactctc tatgccctct
    28501 gtccactagc actgctaatt gtattgattt aggtcttcct atatctataa tagcatctag
    28561 tgaagctctc acagttcttc cagtgtataa aacatcatca actaaaacta ctgtcttatc
    28621 atttacatta aaatctaaat aagttccttt aactacaggt tcaacatgaa ttttttccaa
    28681 atcatctcta tacaaagtta tatcaatatc tcctgtatct acttttgttc cttctatttg
    28741 ttctattttc ttagaaattc tatttgcaat tggcacacct ctagtcttta ttccaactaa
    28801 aactagattt tctacacctt tatttctttc aattatttca tgacttattc tagtaattgc
    28861 tcttgctatt gccttttcat ccattaattg agctttttct accatatcat aattattgtc
    28921 aaagatataa tatatctatt tgacaaatct actcctttct tttaattttt tataaattta
    28981 tttttattta aaatttcaat ttaaacaaca aaaaaacttc tcatcacaag acaagaagct
    29041 cttatataaa aaatatacag tgtaatttag caattgtctt acgatatctc tgtatcgttt
    29101 aaagataatt ttttaatcta tcaactcaaa ttatatattt atatttcaaa tttttcaagc
    29161 ttttttaaac aaaatacttt aatataagat attttattta aatttcacta aatttattta
    29221 cttatataaa aacaaaaaat gatttataag tattatacaa atggttattt tatcttttta
    29281 ataacttcct taaaatactc aggtaattct gaattaaatt cgatatattc cttagtagta
    29341 ggatgtataa atcccaactt cttagcgtgc aaagtttgtc cttttaattt aaatttagtt
    29401 tcttttggac catatacata atcacctaaa agtgggtgat ttatagacaa agcatgaact
    29461 cttatttgat gagttctacc agtttcaagc ctagctctca tatgtgtaaa cttatcaaat
    29521 ctttctacaa cttcaaagtg agtgattgca ttttttccat cttttactac agccattttt
    29581 aatctatcct taggatttct ccctataggt ttatcaacag ttattttatc ttctttaacc
    29641 acaccatagc atataaattc atactcccta gttatagtat gttcctttaa ctgttctgct
    29701 agaaaattat gtgcataatt gttcttagct atcataagaa gaccagaagt atctttgtct
    29761 attctatgaa ctataccagg tctaataact ccatttattg atgacaactt atctttacaa
    29821 tgatataaaa tcccatttac taacgtattt tcatagtttc caggagcagg atggacaacc
    29881 atatcctgag gtttatttat tattaagaca tcatcatctt catatacaat atctattgga
    29941 atatcttgag gtaaaacttc taatacttta ggttctggta tctctataac aatattatct
    30001 tcctctttaa ccagatatct agctttttct actttattat taacctttac ttttccatct
    30061 tttataattt tttgtatata acttcttgac atatcaccta attgttctgc tagataaata
    30121 tcaagtctaa cttcttcttc ctcttcaagt actaaaaact cttttacttc atccattata
    30181 tcacctactt ttactttcaa aaaataaaac atatatacat aaaaacacag ttcctacaac
    30241 tacaaataca tcagctacat tgaatacata ttcccatata actctaaagt cgaagtaatc
    30301 taccacaaaa cctaatcgta ctctatcaac tagatttccc aatgcaccag atataattaa
    30361 tataattccc aaccttccaa atatatgtac tttccttgta ttaagatagt ataatccaaa
    30421 taccgttgca agtaatgcaa caattataaa tatccattga ttattttgta ataatccaaa
    30481 tgctgcacct ctattttcga cataagttaa atgaaataca ttatttatta ttggtattgt
    30541 tgatacatcc accaaattat tcaataccca tattttagac agttggtcta aacctatgag
    30601 tagaattatt attaatatat atagcaatga tttttccccc tataaattat ttaaaaacca
    30661 attttatttg aagcacacta ttattattat acaaaaaaaa tgctctaaag agcaacttta
    30721 ttaaaattgt attttcttaa taaagctaga acaaaattta aaatagtact acttttttta
    30781 tattgtagat ttctacatat ttaaaatcat atcattatgt ataaaaaatt taaaaatacc
    30841 atactaatca atattttttg tgtattttat agattaaaga ttaatgatgt ctgtaattta
    30901 aacttacaat caccactaat cttcaaacaa atatttttac ttttgcactt gcttattttt
    30961 atctaaatgt tgtataagtt cagatactgt tacgaattta tatcctctct cttttaattt
    31021 aggaattact acttctaaag cttgtatagt ttgtgattta tcatttcttt tagtgttata
    31081 atcatgtaaa agtattatat taccattttg gacctttgta aggatagtat ttataattgc
    31141 atctactcca gggttagacc aatctctagg gtctaagttt gaccaaagaa ttatattcat
    31201 tcctttagaa actataatat cacagacact tttactcatg gctctatatg gaggtctaaa
    31261 tatctttggt tctactccta tcacactttt tataatattt tgcgtatctg ttatttcttt
    31321 ttcaacacta ctataactat tttttgcaac atttatatgt gtaaatgtat gattccctat
    31381 ttcatgcccc tcatcatatt gtctttttaa taattctttg ttatattcta ctttttctcc
    31441 aactacaaaa aaagttgctt ttacatcatt tttctttaat atatcaagta cttgtggtgt
    31501 aaaatcctca tctggaccat catcaaaagt caatgctaaa attttttcat cctcatttcc
    31561 tttttttatt attacatctt cataatcgtc ttttatattg tcatttaaac tatatgcttc
    31621 ttttgaacta tcttgattat gtgtattatg attatatgta tatacaccta ctatagataa
    31681 taaacatata catataccac atattatttt cttcatgtca ttacctcttt tctaattcat
    31741 tctagcaata tattttataa tgtcagtttt ttaatctctc aatattccat ttttatttaa
    31801 atcagttaaa tttgttaggt cttctgaata aaagttagaa tcttctacat cagggttttg
    31861 cattgcatca cctaataaat tattttcctt ctttgcacaa tcattacata aacttgtatc
    31921 tggcaatata tccaatcttt ctaaatctat ttgattatga cataaatcac aaatcccata
    31981 tgttccattc tctattttag ataatgctaa gtctatttga tacaatcttc cctcttcatg
    32041 atttattaga ctgttttgca tattttgcat atataaatct gttcccatgt ctcctatatg
    32101 attatcataa ctagataatt ctcctgatga atacttttca cttgtatgtt tagttgtatc
    32161 tccaaataaa gtattatctt tcatatcctc tactaaatta gttaaatttt ctctttcttt
    32221 caataattta tctttatatt ttttagtatc catttttaac acctctaatt ttaatattta
    32281 catcataaaa atagtatgtg gaaaagtaaa ttaaacatac aataaaaata agatttgatt
    32341 cgaaaaatgg aatgtctcaa aatgaaattt ttagttcatt taaccctctt ttttatatga
    32401 aatcaaatat atttttatcc tttcagcaat aaaaaagaga cttatctcta ttttgagata
    32461 gtctcttctt tgaacataat ttaaatacaa ttttttaatt tttatagtta ttattttact
    32521 aacttgccaa tttatgatta ttttgttctt taatatgttt tgctttttta aagaataaaa
    32581 gaccaagaat tattgctaca ataacaccta ttccattaga tatattaact ggtagtctaa
    32641 atccttcttt tgcttgcatt atataactaa atgttactac agtcataaaa agtgctggta
    32701 atgtagttat ataatggttt ttatttgctt tgactaaata tgcagaacca gtccacaaca
    32761 ttatcattgc aagtgtttga tttgcccaag agaaatatct ccaaattata ttaaaatcta
    32821 taaatgtaag agcaacacca actgcgaata atggtaaagc tattaaaaat ctatttttga
    32881 ctgcatcttg attataattc attgcatctg ctattgtcag cctagcactt ctaaatgctg
    32941 tatctccaga agttatagga catgccacaa ctccaagtat agctaaagct cctccaatag
    33001 gccccattaa tccaacagat actgtattta ctactgttgc tgctccacca tcattaaata
    33061 tcacattaag ttgaggtatg ccatggaaat atgacatggc tgctgctgcc caaactaaag
    33121 ctattatacc ttctgcaacc attgacccat aaaatactgg tcttgcctca ctctctttct
    33181 caacacatct cgccataagt ggtgattgag ttgcatgaaa acctgatatt gctccacatg
    33241 ctattgttat aaataagtat gggaataaag gtgttccatt tggattcata ttttgtaaat
    33301 ttatatttgg tatgttatac ccctttatta ttaatcctcc agctacacct actgccataa
    33361 ttaataatgc tgctccaaat aaaggatata ttttaccaat caatttatct attggtaaaa
    33421 cagtagccat aatataataa agtattatca ctccaaccca tatttgtgcg tttacacctg
    33481 tcaatgtctt taataatcct gctggacctg ttacaaatac aactccaacc aatactaaaa
    33541 gaataacact aaatattctc attgctattt ttgcaccatt tcctagataa aggcctacca
    33601 actctgatgc agatgtacct ttgtttttca ttgatagata tcctgaagca aaatcgtgta
    33661 ctccaccagc aaagattgtt ccaaacacaa tccataaaaa tgctgatggt ccaaataatg
    33721 ctccttgtat agctccaaat atcggaccta accctgctat atttagaaac tgaattaaaa
    33781 agattctctt ccatttcatt ggcacataat ctactccatc cctacaagct atagctggag
    33841 tttgattttt gtcattggtt ccaaaaacct tttctacaaa agtaccatag acaaaataac
    33901 ctactataag tactactatt gaacctaaaa agcttaccat aaattcatcc ccttagtttt
    33961 tatataattg ctttataaat ttttatgagt ttaagaaaac tatttccagt tcaaatagta
    34021 atactttttt atgctaatat aaatattaat tacattaagt gcatatttaa gtacatgaaa
    34081 tgactttata tatgcattaa cagcctaaat tcttttactt tgcttctact tacagttact
    34141 tcataatcac tattttttaa ttttaatatg tatgtattat taaaccatgg tataatttct
    34201 tttactttgg ttaagtttac gatataagac ctatgtgttc taaaaaatgt tttatcattt
    34261 ataagatttt cgacttctta tatgccttct tttaatataa acttttcttt ctcagtatat
    34321 ataaatgtct gcctttcatt agcttcacag taatatatat catcaatatc tattacaact
    34381 aatttatcac ctttccaaag gctaatttta tgtgttattt cttctatctc ctgatttact
    34441 gcttccttct tatatttgta taaattacta tttacattaa atagttcaat atcactcatc
    34501 tcactttttt ctaatttatt cagcatagaa ataattctct cttcagaata tggtttcaat
    34561 atataatcaa atacatccaa agaaaaagca tctactgcat aactttcata tgctgttata
    34621 aaaacaattt taggtcttga tttaaactga tttaatgttt ttgcaagtag cattccatca
    34681 agatgaggta tatttatatc taaaaaaatt acatctattt tattttcctg aataaagtct
    34741 aacacctcta taccattggt aaattcactt actacctcga ttccactttt attttcaata
    34801 aaatatctca attcctttct tgctggaaat tcatcttcca ctattatagc tctcatactt
    34861 ctactccttt acataaaata ctatcaaagt cccattatct attcttctta tattaagccc
    34921 tcttccatac atcagtttaa gccttaaatg tacattataa agcccaatct tattttctgg
    34981 catattatct tgatatacat tatttataat ttcctcactt atacctatac cattatcctc
    35041 tattgatatt ctcacagtat tttctaatga atacggtaac ttttttatgg atattttcac
    35101 tacaccagct ctaccacttt ctaaaatacc atgtattatt gcattttcca ctaatggttg
    35161 tataatcaaa cttggaatct ttatatttac atcttctata tcataagata cagttagtaa
    35221 ttctccaaac ctagcttttt ctatctctac aaaagattta acttgttcaa tctctttatt
    35281 tatatcaatt aagttatcac tatattcaag attatatctt agataagttg ataaatttat
    35341 tattaactct ctagccttat ctggactaaa tcttataaat gaagctattg tgtttaggga
    35401 attaaacaaa aaatgtggat taatttgtct ttgaagtgct tttatttctg ctttatttct
    35461 cgcttctata tcttttttat ctttctcaat cccctctact atagatataa caaatcctat
    35521 tccaatctgc ccaactatta aaggtaaata tattccaccg attatttgaa ttgctaaaga
    35581 atgtggatat gatattaagt atataagtaa tatagtaaaa ctttcagaaa tcatgcctac
    35641 caatattcca tacattactc tatgttgttt tggtatcctc ttatatagaa atcctgataa
    35701 tacacctgct agtatacttg ataaaagaca tggaacagat gttatccccc caatatccat
    35761 aaaatatcta tgaatccctg aaaaaactcc tgctgtaata cctaccatcg gaccaaataa
    35821 aatgccacct gatactatag atattattct agtgtttact attgacccca tataatttat
    35881 accactataa gttccaaata tggctaaaaa agtaaaaact aatgctatac agaccaagtc
    35941 attaaaacta tattcttcct tttgaaatat ctgcttaaat atttttaact ttgttattag
    36001 aagaaacaat actacaataa ggcttgtctt ttctattaat gcgattaaaa ttgatgtttt
    36061 cataatcccc ctttaaaaaa taaattttta cacactctaa atttattatg gttgttataa
    36121 attacatatt tgaagtaatc ctatcacaaa actttccaca tttaatcttt taattaaata
    36181 agaaatagtt gtacttctat taacaactaa tactagctag cataagcttg taataacttg
    36241 tagttattat taactttaca attctagtat ttaattttat actttagaaa tctcttttct
    36301 tagaaataat actttcgaat atcatataat acaatattat gtagattctt actcatacaa
    36361 catatttctt taaaaatttt atactattct ttgttcaaat atctcatttg atttaattgt
    36421 gttaaacaca cattatcatc tctctgttat atatttatta tatagccgta tatcgataaa
    36481 gtaaataaat cccatattta aaataatatg tttgacaata aattattata atgtatattt
    36541 accataataa tttgtcaacc ttgaaagtta cttctataat tattgttaaa taattcttct
    36601 aaaaaaaata cctataaatt ttattttctg aaatacatag ttataactat ttttatcacg
    36661 tataagaata aaaattccag aaaattttta ttatctaagt taatataaaa catattattt
    36721 aatataaata aattttttat aataatatat tgatttaatt tactttatta ttgtaaacta
    36781 taagtataag atatactata taaacaagca taaatatacc ttaattctaa ctatccttaa
    36841 tcttatttat ctcctagtgt tattttatgt attttctata aaatactttt gtttttttac
    36901 ttttgaattt tttataggag ggtatttagt ggatatatta atctttgata atgatgtttg
    36961 ttttggaatg aaactaaaag aaaaaataaa taatatttta ataaaagaag gttttgataa
    37021 tgatgttgta agattatatc ataatgcaaa tttgttatta aaagagctta ctgaaaaaaa
    37081 taaagtaaga atatatttta tagttgtaga cgcaaaatat aagataagta atgaattatg
    37141 tgatggatta tggatagcac aaaaaattag agaatcagat tatattagtc caatcatatt
    37201 tcttacaaat catatagaaa tgattttagg gatcttcgat tataggttag aagttatgga
    37261 ctttatacta aaacatgata tggaaattgc tgaaagtaaa ataaaagctt gtattaaaat
    37321 tgctcacaaa agatacatta aagaaaaaaa ttatcgctct aattttttta ctatctattc
    37381 agatttctca ctatggaaaa tttcatttga tgaagttata tattttgaaa caagcactat
    37441 tccacataaa ataaaattag tgactacttc tagaatattt gaattctata aaagtttaaa
    37501 gtctttacca gatttagatg catgctttat ccgtgtacat aaatcttttg tagttaataa
    37561 atatcatatt gtttctctcg acctaaagaa aaataatatt aaaatgagta atggccatat
    37621 atgtcgtata tctaatacat atagaaatat tttaaagaat attattaaaa cataataaat
    37681 agaaaaagga gatgtctcaa gataaaaatt tgaaccatct cttttttata caaaaagagt
    37741 aatccctaag aatcctctat ataataatca tattgtgaaa aaacacatac tgactatatc
    37801 atatataact actattagat attataacaa ttgttcctag attatgattc gataagatag
    37861 ctcattaaat cataaaagta cgaaatagtc tctttaaaat attcagttgt tattttgtac
    37921 tgacatatca tataaataca tatttttatg atatatacct acatatttta tgaaatttat
    37981 ataaaaaatt cttatttaga ttatataatc taaataaatt aaaattcaaa agttaactaa
    38041 actaatattt gtagggagaa taaatgaaaa aatttaggaa acataaaagt attagtaatt
    38101 gtatatctat attgttgata ttatatctaa ctttaggtag tttgttacct aataacattt
    38161 atgcacaaga tttacaaagc tatagtggaa aagtttgtaa tactacttac aaggctccta
    38221 tagaaagatc agaaggattt ttttaaagat aaagaaaagg ctaaagaatg agaaagaaaa
    38281 gatcaaaaac ttgaaaaatc tgaaaaagaa gtattaggat catataaaaa agattctata
    38341 gagataagca aatattctca gacaagaaat tatttttatg attatcaaat agaagcaaat
    38401 tcttgagaaa aagaatatag agaacttcga aatgctatat caaaaaataa aatagataaa
    38461 cctatgtatg tatattattt tgaatctcca gaaaaatttg catttaataa agtaataaga
    38521 acaaaaaatc aaaacgaaat ttcattagaa aaatttaatg agtttaaaga aactatacaa
    38581 gacaaattat taccatacaa cagataattc aactgctgtt caaaatagca atggataatc
    38641 atgaaatact ggattaagta taaacaaagg agaatctgca tatataaatg caaatattag
    38701 atactacaac acaggtactg cacctatgta caaagtgaca ccaacaacaa atttagtgtt
    38761 agatggagat gcattatcaa ctatcaaagc acaagaaaat caaattggca ataatctatc
    38821 tcctggaaat acttatctca aaaaaggatt ttcacctatg gctcttaaca caatgtatca
    38881 atttagttct agactgattc ctataaatta taattaatta aaaaaattag atgatggaaa
    38941 gcaaattaaa ttagaaacaa cacaagtaag tggaaatttt ggtacaaaaa atggttctgg
    39001 acaaatagta acagaatgaa atagttggta agacgatata ggtcaaattg acagtatttc
    39061 tgtatctatt atattagata cagaaaatga atcttacaaa agaagaatta ctgctaaaag
    39121 tttacaggtc ccagaagata aaacacctga acttacaatt ggagaagcaa ttgaaaaaac
    39181 ttttggcact actaaaaaag atggtttgtt atattttaat aatataccaa tagatgaaag
    39241 ttgtgttgaa ctcatatttg atgataatat aggcaataag attaaagaca gtttaaaaac
    39301 tttgtctgat aaacaaatat gtaatgttaa acttgaaaga ggaatgaata tacttataaa
    39361 aacaccaact tactttacta attttgatga ttacaataat taccctagta tatggagtaa
    39421 tgttaatact acaaatcaag atggtttaaa aggctcagca aatagattaa atggtgagac
    39481 gaagattaaa atacctatgt ctgaactaaa accttataaa cgttatgttt ttagtaaaat
    39541 tcaaaaaatc ctttaacata taattcaata attgtaaata taaaagtaaa agaagaaaaa
    39601 acggattatt tggtactaga acaaggatat acaaaattta gttatgaatt tgaaactact
    39661 gaaaaagatt cttctaatat aaatatagca ttaattgata gtgatacaat atacttagat
    39721 aacttatcta ttacagagct aaatagtact cctgaaatac ttgatgaacc agaagttaaa
    39781 attccaactg ttcaagaaat aatagttaca cataaaatat attctgcaga ttttaaattt
    39841 aatccaagta caataaaggc ttatataaat ggtatgtatt ttacaccaac ataaactaat
    39901 aaagaagctc tcgattatgt ccaaaaatat agagttgaag ctactttaca atattttgga
    39961 tttaaagata ttgaaactaa agataaagaa ttgcgtaatt atttaggaga tacaaatcaa
    40021 cctaaaactg attgtgttaa tcttaggagt tattttacag gtggagaaaa tattatgaca
    40081 tacaagaaat taagaatata tacaattact caagatgata gagagttatt ggttcttagt
    40141 gttgattagt atcaaatgat ttaaatttat ccccacaata caatatgtat ttaccttata
    40201 aagtattaaa aaattaactt tataaggtat tttttattta aatattatct tcttctaata
    40261 tattcttttt tagaactata ttgtattaaa aagataatta tctgtatcat atcacctata
    40321 tataaacata aattcattta attatataaa tttaaaaaat atttttttga tatattattg
    40381 acaattttca ttataagagt taaaattaac tcataaatta catattgggt ataaaataca
    40441 atgtatgtta gaagttgttt cagaaggcta tggttttctg atttttttat gctcagtagt
    40501 gatatgcaaa aaagacaaag ttgacatcag ttaactttta cctttattct actttcccca
    40561 aagtaagaat aatctaaccc ctttgagatg agcaaaagct catctttttt tattatcaaa
    40621 agatgctgtc tcaaaataga gataatcctc tttttcattg ttgaaatgat taaaatatat
    40681 tttatttcat acaaaaaaga gtgcctcatg aacaaaagtt cactttgaga ctctctcttt
    40741 catatcatat acctttttaa taactttaaa atttatttat caagtaaaga tagctaataa
    40801 ttagtatctt ctctctataa atccaacttt tttataaact tttctaagag tttttctagc
    40861 ttgtttttcg gctttttctg ctcccatact atatattttt tctaaataat ctttattatt
    40921 aagaaggtca acatattttt ctcttatagg tcttaaagct tctgatacta cttcagccac
    40981 atcttcttta aatatgccat aaccctttcc ttcatacatg tttacaatct cttctacact
    41041 ctttttactt aaattagaat atatatcaat tagattttta atacctggtt gttcatcatt
    41101 gtattttaca acacctaaag aatcagtaac acttcttttt atctttctct ttatagaatc
    41161 tgaatcatct gctaatagta taaatgcatt ttcatttgaa tcggatttac tcattttttt
    41221 agttggctct tgcaaactca tcactcttgc tccacctttt gggtagtatc cttctggaac
    41281 tacaaaagta ggtgaaaatc tattattaaa tctatttgct aaatcacgag ctaattctaa
    41341 atgttgtttt tggtcatctc caactggaac taggtcagtt tgatacaaca atatgtcagc
    41401 agccataaga actggatatg tgaataaacc tgcatttaga tttgcttcac tcttttgtga
    41461 tttatctttg aattgagtca ttctacttaa ttcacccata taagtcatag tattcaatac
    41521 ccacataagt tcaacatgct ctttgacatg tgattgtata aatatagtat ttttttctgg
    41581 gtctaatcca caagctatat attgagcaag taattctata gtattggctc ttaaaacttt
    41641 agggtcttga ggtactgtta ttgcatgcaa atcaactata ctataataac aatcataatt
    41701 gtcttgaagt tcagtccagt tttttatagc tcctaaataa tttcctaaag tcattttgcc
    41761 tgatggctgt gcaccactaa atataacttt tttttcactc atttttttgt acctccttat
    41821 atatttatat tgattttaac atatttaaaa ctatgtcttt agagttattg gcagcatcat
    41881 gtacaaatac atcataagta acatcagcac ttccatctgc cttatcagac attgctctta
    41941 ttattacaaa tggagtatta tttaaatagc atacatgagc tatagctcca ccttccattt
    42001 ctccacaata tccaccaaaa tcatttacta attcttcttt taattcttta gaatttataa
    42061 atttatctcc tgtaactact cttcctttta atactttatg agtctttact tcttccacag
    42121 aagatttgta agcagcatct ataagtcttt catctgcttt aaatacagaa gtcttcattc
    42181 ttggtataac tcccttaggg tctccaaatg ctgtagtgtc tacatcatac tgtatagcat
    42241 cagttgatat tactatatca ttaacatcaa gtttttcgtt taaagctcct gcaacacctg
    42301 tattaacaat agcatcaacc ttaaattcgc ttattagtat ctgggcacaa agagcagagt
    42361 taaccttacc tatgccacac tttactaaca ctacattctt accttccaaa attcccttat
    42421 aaaattctaa gctagctttt tttatagtct ctctaatatc cattaaatca actaaaatac
    42481 taacttcttc atccattgca cctataattc ctattacgtt catttttcct cctaaatttt
    42541 ctatattaaa aaatccgtcc taaatcaata ggacggatat attttccgtg gtaccaccta
    42601 aatttatata cactcaaaat tgaatatata tatcttaata tatgctataa cgtgcataca
    42661 cgatatcaaa tactctgtaa tattaaatta catttcattt tactcctcaa agacccattc
    42721 aataaatact ttttattaag ttctcagcat ccttaattct ctgtgcaaaa atatatttat
    42781 ttactatttc ttatctcagg atttaattta ttaatttctt tattttataa tattccttac
    42841 actataaatt gtcaactata tattataata tccaaaagaa atttttaaaa tacattttat
    42901 ttagctgttt tcttttcata tctattccat aaccatagac cagataatat aactagaaga
    42961 cctccaccaa tcatctctat ctcatatata acattttcag caaatgtagg cataactggt
    43021 gctgcagata ataatatacc cactacagtt acaactaaaa ccatttttgc taaacctagg
    43081 caagtacttg ttgattttgc catagtataa ggtctaattt catctggtct ctcttttctt
    43141 agtttaatat aagaagcata taacaataca tatgggaata atgcagttaa tgctgtcata
    43201 gttacaagaa cattgtatat agcatcaaca gaaggcatta atgtagtcac caatagtatt
    43261 atagtaacta ttattgcttg aagtataact gctttagaag gtatcttgtg ttcatttacc
    43321 tctactaaac tatctggaaa tattcctttt ttaacactac caaataacat ttttattggt
    43381 gatgctatgt aaagaactat tgctcctaat actgaaaaag ctataccaaa agctattaat
    43441 tgaaccaacc aagaaccaat tcctaaagac gcacaaacag cagaaagtgc atctaataca
    43501 cctttagaag cagtaatttg gtctggtggt attatcattg ttattgcaat tgaacctaat
    43561 acatataatg aagctactat tccagctgat attaatattg ccttagggaa gttcttttca
    43621 ggcttatcta tttctgttat gaagttagca gcagtctctg ctcctgctaa accaaacatt
    43681 atagctgata tagcaacaaa tgcatccata ttaagttttg gagtcattga agctattgtg
    43741 tatgtagaag ctatatcatg ttttttcaac acaaatactg ctacaaatga aaatattata
    43801 agtatagcac ttggtattgt tgaacctaat gcacctgtat tagtaaatat ttttgtaaaa
    43861 cttattcctt tagttgctat aagagataat gcccagaata ttattaaaga taatataagt
    43921 acaaacatct tattatcagc aagggctgga tttcctattg tataagaaac atttactgct
    43981 aagaaagtta aaaatgaaga ataccaaaat attttagctg tccagtttaa ccaagaaact
    44041 aaaaatccat acttttctcc aaaggcttcc ttaacccaat cgtaaagacc tccatcacct
    44101 ttttgatatg tagaagctaa ttcagcacaa attaaagctc ctggtacaaa gtatataaat
    44161 gagaataata accacatagg tattgcacct aatcctaaac caaagcttga tgctgtagat
    44221 ttagctatcc atcttatacc atataaagct gatacattca taaataccaa actccatagg
    44281 cctatttttt tcttttccat cctaatttaa tttcccctct ctattaatta ttttaaattt
    44341 aatttcaaaa ttaataaaat agattcatta ttattaaact gatatttttc aaaatatttg
    44401 catattcaaa aacagtttta ataataaacc ttaaatactt tgaaatcaaa tcttattaaa
    44461 aatgataaat ttaaaagttt taccacttta attctaatga tactaaattt ttaaaattgt
    44521 ttcaatatta ttttattata aaattttatt tattattttt cattagtata taaaaag
//
LOCUS       DAYAOY010000025        43086 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYAOY010000025 DAYAOY010000000
VERSION     DAYAOY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.025"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            273..1490
                     /gene="ilvA"
                     /locus_tag="ACGI6B_001454"
     CDS             273..1490
                     /gene="ilvA"
                     /locus_tag="ACGI6B_001454"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HFG9750903.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1585..1965
                     /locus_tag="ACGI6B_001455"
     CDS             1585..1965
                     /locus_tag="ACGI6B_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9750904.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2249..2731
                     /locus_tag="ACGI6B_001456"
     CDS             2249..2731
                     /locus_tag="ACGI6B_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9750905.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2752..3567
                     /locus_tag="ACGI6B_001457"
     CDS             2752..3567
                     /locus_tag="ACGI6B_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9750906.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3618..5168
                     /locus_tag="ACGI6B_001458"
     CDS             3618..5168
                     /locus_tag="ACGI6B_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9750907.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5265..6617
                     /locus_tag="ACGI6B_001459"
     CDS             5265..6617
                     /locus_tag="ACGI6B_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9750908.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6748..7506
                     /locus_tag="ACGI6B_001460"
     CDS             6748..7506
                     /locus_tag="ACGI6B_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9750909.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7553..8272
                     /locus_tag="ACGI6B_001461"
     CDS             7553..8272
                     /locus_tag="ACGI6B_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HFG9750910.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8396..9796)
                     /locus_tag="ACGI6B_001462"
     CDS             complement(8396..9796)
                     /locus_tag="ACGI6B_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFG9750911.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9800..10690)
                     /locus_tag="ACGI6B_001463"
     CDS             complement(9800..10690)
                     /locus_tag="ACGI6B_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9750912.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10969..11616
                     /locus_tag="ACGI6B_001464"
     CDS             10969..11616
                     /locus_tag="ACGI6B_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9750913.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11941..14580
                     /locus_tag="ACGI6B_001465"
     CDS             11941..14580
                     /locus_tag="ACGI6B_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFG9750914.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14638..15741)
                     /locus_tag="ACGI6B_001466"
     CDS             complement(14638..15741)
                     /locus_tag="ACGI6B_001466"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9750915.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16101..16922
                     /locus_tag="ACGI6B_001467"
     CDS             16101..16922
                     /locus_tag="ACGI6B_001467"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9750916.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    17001..17212
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17299..18615
                     /gene="argH"
                     /locus_tag="ACGI6B_001468"
     CDS             17299..18615
                     /gene="argH"
                     /locus_tag="ACGI6B_001468"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFG9750917.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18864..19202
                     /locus_tag="ACGI6B_001469"
     CDS             18864..19202
                     /locus_tag="ACGI6B_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HFG9750918.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19329..20618)
                     /locus_tag="ACGI6B_001470"
     CDS             complement(19329..20618)
                     /locus_tag="ACGI6B_001470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9750919.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20698..21402
                     /locus_tag="ACGI6B_001471"
     CDS             20698..21402
                     /locus_tag="ACGI6B_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFG9750920.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21494..22540
                     /gene="selD"
                     /locus_tag="ACGI6B_001472"
     CDS             21494..22540
                     /gene="selD"
                     /locus_tag="ACGI6B_001472"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:21542..21544,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFG9750921.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22591..23970
                     /gene="selA"
                     /locus_tag="ACGI6B_001473"
     CDS             22591..23970
                     /gene="selA"
                     /locus_tag="ACGI6B_001473"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFG9750922.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VEELRKAFN"
     gene            24084..25967
                     /gene="selB"
                     /locus_tag="ACGI6B_001474"
     CDS             24084..25967
                     /gene="selB"
                     /locus_tag="ACGI6B_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFG9750923.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26072..28810
                     /locus_tag="ACGI6B_001475"
     CDS             26072..28810
                     /locus_tag="ACGI6B_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HFG9750924.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28929..29873)
                     /gene="manA"
                     /locus_tag="ACGI6B_001476"
     CDS             complement(28929..29873)
                     /gene="manA"
                     /locus_tag="ACGI6B_001476"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFG9750925.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            30046..31407
                     /locus_tag="ACGI6B_001477"
     CDS             30046..31407
                     /locus_tag="ACGI6B_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9750926.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31495..33384
                     /locus_tag="ACGI6B_001478"
     CDS             31495..33384
                     /locus_tag="ACGI6B_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9750927.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33399..33854
                     /locus_tag="ACGI6B_001479"
     CDS             33399..33854
                     /locus_tag="ACGI6B_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9750928.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33913..34230
                     /locus_tag="ACGI6B_001480"
     CDS             33913..34230
                     /locus_tag="ACGI6B_001480"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9750929.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34255..35517
                     /locus_tag="ACGI6B_001481"
     CDS             34255..35517
                     /locus_tag="ACGI6B_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9750930.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35622..36686
                     /locus_tag="ACGI6B_001482"
     CDS             35622..36686
                     /locus_tag="ACGI6B_001482"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9750931.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36740..37918
                     /locus_tag="ACGI6B_001483"
     CDS             36740..37918
                     /locus_tag="ACGI6B_001483"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9750932.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38109..39167)
                     /locus_tag="ACGI6B_001484"
     CDS             complement(38109..39167)
                     /locus_tag="ACGI6B_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFG9750933.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39234..39605
                     /locus_tag="ACGI6B_001485"
     CDS             39234..39605
                     /locus_tag="ACGI6B_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFG9750934.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39642..40316
                     /locus_tag="ACGI6B_001486"
     CDS             39642..40316
                     /locus_tag="ACGI6B_001486"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFG9750935.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40565..41404
                     /locus_tag="ACGI6B_001487"
     CDS             40565..41404
                     /locus_tag="ACGI6B_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFG9750936.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41648..41744
                     /locus_tag="ACGI6B_001488"
     tRNA            41648..41744
                     /locus_tag="ACGI6B_001488"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41682..41684,aa:Sec,seq:tca)
     gene            41894..42601
                     /locus_tag="ACGI6B_001489"
     CDS             41894..42601
                     /locus_tag="ACGI6B_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFG9750937.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42743..>43086)
                     /locus_tag="ACGI6B_001490"
     CDS             complement(42743..>43086)
                     /locus_tag="ACGI6B_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9750938.1"
                     /translation="TFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSS
                     SLEFIKKISPDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEI
                     IFTKYNYEIDT"
BASE COUNT        16884 a         4740 c         7471 g        13991 t
ORIGIN      
        1 taaaataata taaaatataa attttaataa aaataataaa gtatatatta ttgctttaat
       61 aagaatgtaa aaaatatttt taaattatca tttaatgtaa tttaaatgaa ttgaagatat
      121 atgtttattg tatgcaattg ttgtattttt aggttttttg tgattaatta aaaaattata
      181 aaaatcgttt acatgttgat ttttaaaaaa aaaagatata gaattatata gaaagtaata
      241 aacaaaaatt ggatttacgg gagtggaatg aaatgatgaa tgttacctta acagatgtga
      301 aagaggctag agagaccatt aagaacatag taaaaagaac tgacttactt gaaagtgtta
      361 aacttagcga aaaaacagga gcaaatgtgt tctataaatg tgagaatctt caaaaaacag
      421 gttcttttaa attaagagga gcttgtaaca aaatagcgag tttaacagat gaagaaaagg
      481 caaatggagt tatagcttca agtgcaggga atcatgctca aggagttgca ttgggagcaa
      541 aaatgactgg tataaaatct acaatagtaa tgccagcaac tgcgccttta gcaaaagtta
      601 gtgcaactaa aggttatgga gcagaagtag ttttaaatgg tgcggtttat gatgatgcat
      661 atgcaaaagc tgttgaaata caaaaagaaa caggagcaac tttcttacat ccattcaatg
      721 ataagtatgt tatagcagga caaggaacta tatctcttga aattttcgaa caattagaca
      781 ataaagttga tactatacta tgcccagttg gtggaggagg aatcatctct ggtgttgctg
      841 ttgcagcaaa agcattaaat cctaatgtaa aaatagtcgg tgttcaaact gcaaatatac
      901 cttcaatgaa agagtcaata aaaaatggaa aagtaacaac agcatttaat gatactacaa
      961 tagctgatgg tatagctgtt aaaactccag gagatttaac ttttgaaata ataaatgaat
     1021 tggttgatga aatagtagtg gttgaagaga cagaaattgc agaatcaata ttatttatga
     1081 tggaaagtca aaaaatagtt tcagaaggtg ctggtgctgt atgtacagct gctatattaa
     1141 gtggtaagta tgtaccagca aaagatgaaa atgtagtttg tataatatct ggtggtaata
     1201 tagacataaa tacgttatat agaataatag gtgttgcttt agctaaagaa ggaagaagat
     1261 attcatttag tacaattatg gaagataagc caggaaactt tgctgagtta actagaataa
     1321 taagtgagaa tggtggaaac attttaagtg ctaatcaagg taaattatca gcaggggaag
     1381 ctctaggaaa acaaagtgcg gaatttatat tagagacaat agattatgac catatagcta
     1441 gaataaagaa agctatagaa gaaaaaggtt ttaaaattat agaattataa taaaaagtgt
     1501 atataatata caagaaaaat agtataatag tgtatgaaga tacattttat gtgtattaga
     1561 ttaacgaatt aggaggattt taaaatgaaa catgaagtaa tacatactaa tgatgcacca
     1621 gcagctttag gaccatattc tcaagctata aaagctggaa acttgttatt tgtatctgga
     1681 caagttcctc ttgtaccaga gactatggaa gtagttgaag gagatgtaca agctcaaact
     1741 gctcaatctt taaaaaatct taaagctata ttagctgaaa gtggagctga tttttcaaat
     1801 gtagttaaga ctacagtatt tataaaagat atgaatgaat ttggggctat aaacgaagtt
     1861 tatgctgaat attttggaga aaataaacca gcaagagctt gtgtagaagt tgctagatta
     1921 ccaaaagacg ttaaagttga aatagaagta atagctgtat tataaaaata caatattttt
     1981 aatttgaaaa ggctgaaaaa ctatattcta gtgattcagc ctttttataa aaaaataaat
     2041 cacaataaag aacatgataa atattgtcga attaaaaaat aaaattaaaa taaatattgc
     2101 aatatttttg agaaggtttt ataatgagta tataatttga aacgtgtaac tattaaatta
     2161 ggcatgagtt cttggatatt tttatcctga ggattcatgc cttttttgta ttgaaattca
     2221 aaaatctatt taataaggag gaagtactgt gttaaatttt ttcaagaaaa ataaaagcta
     2281 taagttacat gcagtagtga gtggtaacag cattaatatt gagaaagtaa acgattctgt
     2341 gttttcaaaa aagttaatgg gagatggagt tgcaattatt cctaatagta atgttgttgt
     2401 agccccttgt aatggtaaag taactgtgct tacagaatca aagcatgcct ttggtatggt
     2461 ctcagatgag ggtgttgaaa tattagtcca cataggaatt gatacagtta gtttacaggg
     2521 tgaggggttt aaaaatgaag tgtctcaagg agatactgtt aaaaaaggta gtccaataat
     2581 aagttttgaa agagaaaaaa taaattctca gggaattgat tgtacaacaa taataattgt
     2641 attaaatcat agcgaatttt cagaaataaa ttgcatggta gaaaatgaag ttgttgcagg
     2701 gcaagatact gttattgaaa ttatgaaata ataggaaggg attaattcaa tatgagagta
     2761 gtaaaaatct ttaataataa tgcattatct acagtaacta atgataggaa agaagctatt
     2821 ttactaggtc ttggtatagg ttttaataaa cgtccaggag ataaagttaa tgaagataaa
     2881 atagaaaaga tttattatgt tcaagaccac atgcaaacaa aatttttaga attattaaaa
     2941 aatgttagtc cagaagtgat ggatgcttca caacaaatta taagtcttat accaggaaat
     3001 gagggaaaat ttaataataa agggatatta tctttagtgg aacatattag ttttgcaata
     3061 gatagaatga aaaataatgt ttttttacct aatttaatgc ttttggatat aaagatgatg
     3121 tattcaaaag agtttgaatt aggagtaaaa gctttagaaa ttatatatag agtatgtcat
     3181 atccatttgc ctgaagatga agcaggttac atagctttac attttgtcaa tttacaatca
     3241 aatgataatt tggcatatga tactttaaaa tttgtaaaag gtagtattga tttgattaaa
     3301 gaatgttatg gattagaatt agacgaatcg agtttaagta cattacgttt tagaactcat
     3361 cttaaatttc ttgctcaaag aatttttaag aatgaaattt gtcaggatga taaaatgatt
     3421 gaaatgtatg attatttaat taacaatcat cctaaaaata aagaatattt agaaaagtta
     3481 aatgtatata ttgaaaagga gtttaggtat aaattagata aacctgaaaa aatttatcta
     3541 ctactacacc taacaaaaat cttataagaa tgagatgaat tagtaataga aatatataat
     3601 tttagggggg gataagattg aaagaaaaac tacttggagg actggaaaca tttgcaaagg
     3661 caatggttca gccattaatg tatttatcga gtgcaggaat attaatggtg gtaggtgtac
     3721 tacttacaaa tgatatgtta ttaggagtac ttccattttt taaatgggag ccaattcaaa
     3781 tatttggaag cctaatttat aactgtataa tggctattat taataatctt gctgttatat
     3841 ttgctatagg tattcctgct gcactagcaa aaaaagagaa gcacaaagca gcgttaatag
     3901 gatttatgag ttatataata taccttacag cttccaacac tatgttaaca tcactaggtc
     3961 aactagcaaa agaaagcaag atgcttggac tgatagggac tggacaagct actattttag
     4021 gtattcaagt tgtagataca agtgtatttg gtggaattat tttagggtgt atagctggat
     4081 atgtatttaa tcgtacaagc aacaaaacat ttaaaggagc tttacagata tactcgggag
     4141 caaattttag ttttatatgc atgttctttg tatccatttt aattggagtt gtgagtaact
     4201 ttatatggcc attagctcaa caaggaatag cttctcttgc aaatgtcatc aaagatacag
     4261 gtacatttgg tttattctta tatggttttt tagagagaat attagtacca actggacttc
     4321 atcatttaat ctatacacca tttcagtttt ctgatttagg aggagtgctt aaagttggtg
     4381 cagatacagt ttcaggagcc tatgctatag ttatgacaga aatgaattta ccagttgagc
     4441 gtttctcaga tagtatatac tacatggcaa ctggatttac aaagatgttt ggttatatag
     4501 gtataggagc tgcctttatt tatactgcat ataagcaaaa taagaagaaa gttaaggcaa
     4561 cgattattcc actaataatt acggcagtat tagcctctgt aacagaacca ttagacttta
     4621 tgtttgtatt ctgtgcacca tttttattct ttgtacatgc agttatagct ggtcttttta
     4681 tagctttatt aaaaatattt gatgttactg cattatgtgg agggaactta ttagtatcaa
     4741 ttataatgaa tactacagta ggcgtagaaa aaacacactg gccagtaatg atgatattag
     4801 gacttgcaca aattattgta tattttgtag tatttagttt tttaattaag aaatttaatt
     4861 ataaaactcc aggtcgtgaa gatgagagta cagctacagg cgaagaacaa tcaaaacaac
     4921 ttaatttaga tgcagggata gaaaatataa ttgatgggtt aggcggaaaa gaaaatatta
     4981 atacagtaga aaattgcatc actagattac gtgttaatgt aaaagatgaa agtaagatta
     5041 atgaagatat aattaattta acgccaaata gcgggattgt aaggaaagga aaagatatac
     5101 aaataatttt tggacttcat gtgcatgagg taagacgtgc agtagaagat tttttagaag
     5161 cttattaaag atatatattt atatagcaag ggtaaataac agctttaagt gctgtttact
     5221 atttattaaa gatacatata atttatagag gaggaataat tattatgatt attactattg
     5281 ttggtggagg aagcacattt actccaggta ttgttaagtc aattgcatta agagaaaaag
     5341 agttagagct tgaggaaatt cgcttatttg acattgataa agaacgccaa gataaagtag
     5401 ctgtagttgt caactggata ctaaaagaag atgttaaaac agatgttaaa ctaactgtaa
     5461 ctaattgtga aaaagaagcg tatacagatg ctacatttat atttgcacaa atgcgtgttg
     5521 gtaaatatga aatgcgtgag caggatgaaa aaattccatt gaaatatggt tgtgtaggtc
     5581 aagaaacttg tggttgtgga gggatggcat acggactgcg tacaatacaa ccaatgataa
     5641 aggttattga tgatgtagag aaatatgcta aaaaagatta ttggatatta aattactcta
     5701 atcctgctgc aatcgtttct gaagcttgtc gtaagttaag accaaatgca aggattataa
     5761 atatctgtga tatgccaata gctattatag atatgatagc ggggtcatta aatatcaatg
     5821 acgttcataa tatacgctat gattattttg gtctaaatca ttttggctgg tttacttcaa
     5881 tagattataa acatagagac ttaatggaag agattaagga atacataaaa gaaaataaaa
     5941 tattattacc aaaatcttat atagaaaact taaataaaag tcgtagtaca ggaaatcgcc
     6001 atgctgtaag tagctggata catgtttggg aatctgttta tacaatgtta gaaagcttcc
     6061 caaaatatct gccaaacact tatttaacat actatctttt aagtaaagaa acagtggaac
     6121 attcaaacat aaatcgtact cgtgcaaatg aggttatgga aagccgtgaa aaagacttat
     6181 ttgaaggtgt gagacattac ttagaaactg gagaaatttc tgaaaaagca ttttatgcag
     6241 gttcacatgg agattggatt agtgatttgg cagtttcaat taaaaatgat acacgtagtc
     6301 gttttttagt tataactgaa aatcgtggag ctataccaaa catgccatat gatgcaatgg
     6361 tagaaattcc agcatatatt ggtaaagatg gtccagaagt tattgctcga ggtccaatac
     6421 ctctatttca acaaggatta atgatgcaac aacttaacag tgaaaaattg atagtagaag
     6481 cttatataga aaatagttat gaaaaagctt tacaagcatt cacattaaat aaaactattc
     6541 caagtatgaa tgttgctaaa gaaattttgg atgaaatgat agaagttaat aaggaatact
     6601 ggccagaatt aagatagtag gataaaggaa agggttttta tttaataatt agatatgttt
     6661 tcagagtctt ttatcaaaat gtattcacaa acatatggca atatattaaa ataaagagtg
     6721 atatttttag aagaatgggg tgatgcaatg aacattataa ataaaatttt ttgggaagaa
     6781 gataggattg gcatgattac aaataattta gaggcagata cacatagcca ctgtatgtta
     6841 caactgttct tagggataga agacagcatt gaaataacag tcaatgaaaa attagttaaa
     6901 tgtaattgta tcatagtaga taaaaatatc tctcattcat tttcagcaag aaaaaaagtt
     6961 tattactctg ctattattga acctacatct atatatgcag aacagcttac ctcaaaaatg
     7021 aatgattttg gctactggat atgtgacaat gatggattag agaaattacg tcaacaagga
     7081 acttttttga tagataactc tagtaaagaa caatatttaa gatttatgga aatgcttaat
     7141 aactatttga atatacctat aactctcaaa cattatgatg acagaattac tgaattgctc
     7201 aatttattac gtacttgtaa ttgtgataat catactattt caagttttgc agataaagtg
     7261 tccctatcag caagtagatt atcacatctg tttaaagaac aaatagggat acctttaaaa
     7321 agctatatct tatttcatca aatggagtgt gcttttagag aattactttc tgggaaaaat
     7381 gttaccgaag cttctatgac tgctggattc gatactccat cacactttgc agggacagtt
     7441 aagcgaatga tgggaatgcc tgtttcttta tctttgaaag atagcgaatt tttgaaagta
     7501 tattaaatat tctcgttata taatgaagtt gtactaagaa aggggctttg ttatgaataa
     7561 agaatattta gaggaaacaa aaatgttaaa ttataatgaa ccacaactta agcttttggt
     7621 atcatcaaag aattggttgg aattagatga ttttcataaa ataaaatcta tttatgagtt
     7681 tgtacagaat gatattttat ttggatataa tgcttctgat atgttaagcg caacacaggt
     7741 attaaatgat ggtatgggac aatgcaatac aaaagctact cttttaatgg cattattacg
     7801 tgcagtgaat atcccttgta ggcttcatgc ttttgatgta acaaaagatt ttcaacgagg
     7861 agctacatcc aaattgattt cactattagc accaaaatat attcttcata cttgggtaga
     7921 ggtattttat caagataggt ggattgctct tgagggtgtc ataactgata aaaaatattt
     7981 agaagcaatt cagaagaaat tttttaatca tggtggaaca tttaaaaagt atgcaatagc
     8041 tacaaatgat ttgaaaaata catctattga ttgggatggt aaagatactt ttatccaaaa
     8101 agaagctatc gtgtatgatt atggtatttt tccatcacca gacgtatttt tttctactca
     8161 ttcacaacat atgtcaaaat taaaaaattt catatatgta catcttatca gaaaaataat
     8221 gacaaaaaat gtatgtaagg ctagaaacaa ttatattgat aaaaacgaat gagtgtttaa
     8281 gaggtaacta tataggaaat atatagaata attaaaactg tactggttaa aatgaataaa
     8341 aaggagttat cacagagcaa acttaaattg ttcataggat aactcctttt ctgtattaac
     8401 ttctaataat ttgatgtctt tttataaaac atatagatac aattaaacca gtaaatgcaa
     8461 gagtagctgc tattgaaaat gcaacatcta ttccatgaat gttagacagc atgtttgaac
     8521 tcatatgagc tttcttagta gcacttgtca ttatagtaat aagtatagca gaacctatag
     8581 ctccagatat ctgacggagt gtgttattaa ttgctgtggc atgagatata agttctctat
     8641 ctaatgtttt aattccccat gtagttaatg gcattaaaac cattgaaata ccaatcatac
     8701 gaatacaata catcaatgac acatgaatta aagatgtatc tctgcctaaa aaagcgaagg
     8761 ataaagttcc taaaaacaaa catcctgttc ctaatattga tagagcatga ggtccatatt
     8821 tatctaaatg acgtcctgca attggattga gtacaaccat caatatagca ccaggtaaca
     8881 ttaatgaacc tgaagataat gcagaaaatc ctcttacaga ttgaacgtac agaggaatca
     8941 ttaatgtagc agacatcatt gaggcatata caatcataat taatattgta gaaataagaa
     9001 atggtttatt tttaaaaacc ctcaattcta aaaaaggttc tttagatttt aattgacgca
     9061 atgtaaataa aattaatgac ataattccta ttaatattgg taaatatgtg gcaatattag
     9121 tccacccata attaccttga ttagtaaatc caattaataa accaccaaaa cctagagatg
     9181 atagtactat tgaaggtata tctaatttta gtttttgagt ttccccaaca tttcttaata
     9241 gaataaatga tagtataaca tctaatatag caatagggga aagaaacaga aataaactat
     9301 gccaaccaaa tgaatctact aaccatcctg ataaagttgg acctattgct ggagcaaatc
     9361 ctactgtaat accaactaaa ctcatagctg caccacgttt ttcctcaggg cataaataga
     9421 gtataataac ttgtaaaagt ggcattagag aaccagaacc cattgcctgc aacacacgag
     9481 caatcaacat agttgaaaaa ttattagaaa aaagtgcaat aatacatcct atagaaaaga
     9541 tagacattga tgtaataaat aactttctag tactaaatcg atttattaaa taagctgttg
     9601 tgggtacaat aatacctgca ataagtagat aaattgttgt taaccactga ccaatatcag
     9661 cagtaatttt aaaatctgac attatattag gtaatgctgt tgacaatagt gtttgactga
     9721 gagaacttat aaaagcacca gtcattacaa ttgctattat taaatttcct tttttattgt
     9781 tactttgggt ttccataatt tataacgtct ccttttttaa ttgattttta atgctgttca
     9841 ataaaagtac ttttggtctt aaagtgtctt ctttttctaa atatgcaatc attgcatcca
     9901 taattaaaga atcatattgt tgattttttc cttcgacgta taattttttt attgcttcta
     9961 gtaatttagg attttcaagg agagaagtat cagttttgat tagttcttct atatcttgta
    10021 tcgttttaaa aaatagcgat ttattatgaa gggaaaagta aacttcatct tcttgtttat
    10081 gtaaaaacat tgatttttca attaaaggat ttagattatc catattttct acaactaaat
    10141 ctagaatttg tatagtaaat ttggcaataa agtatacatt tatttctctt ttttgtacat
    10201 gaataatgta tataaactga tgaattagag catccaataa gaaaactaaa tcaaatataa
    10261 ttttttctat tttatcacca tagttatcaa ataagcttag tttgtattca ttcaataagt
    10321 tagctcttga ttttgtttgc aatttacttg tagccttacg ttgctcttca gaaaagtttt
    10381 ctcttatata ggtatttatg gtagttctag aaaaaatatt tttccataca gcaattatct
    10441 ttctgtttaa cttttctgat gatggaatgt cattattttc atttatggaa tgtataattt
    10501 ctaaaaactt ttcatcataa taattgatta tttcgcagat aaaactttcc ttagtttcaa
    10561 aatgcttgta aaaagtagct ttagacattt tacactcttt tacaacatcc gatattgaag
    10621 tattttgaaa acctttttgg tcaaaaattt tttgtgcaat agttataatg agcctttgtt
    10681 tttctgtcac tttattatct cctttcacgc aaaaacgttt agtactatat agtacctaca
    10741 agttttgata aatatattat acatcatttg gaaaaaaaat caatatatag actgtaatat
    10801 gaaatttttg aaaaatttgt tgttattttt agtcgtataa aaaagaaact taagagtttc
    10861 ttaagaattg tttaaagaag ttgcaataaa ttagtggtat gattatagta tgaaagagaa
    10921 tgtcttgaaa tttttctgta tcaaattcca attatggagg tgtagtatat gaaaaagaaa
    10981 atttgtgtag caataatttt tgctctattt atagtttttg tagaaatgtt tattgatagc
    11041 tctagcgaag gaagtaaaat tatgacagct gaattaagta aatacaggaa aggcgtaaat
    11101 gtattatcaa taaacaatat tatagtagcg aataaaaaat atagtttgcc caaagattat
    11161 tctcctcagg aaagcagtga agcgagagat gctttttata aaatgaataa agatgctcaa
    11221 aagtcaggat taaatttaaa agcttttagt acatatagaa gttatgaata tcaagataga
    11281 ttatttaagt catatgtaaa agaacatgga gaaaaagaag caaatagatt ttcagctaaa
    11341 cctggagaga gtgagcatca aactggactt gcttttgata ttggaggaga tgaccagtct
    11401 tgttgggcaa ataaaaagtt caacaataca aaagaagcta agtggttgta tgaaaatgca
    11461 tataaatatg gttttatact tagatatcca gaaggtaaag agaatataac aggatatatg
    11521 tatgaatctt ggcactatag atatgttgga actgaacata gcaaaaactt tgcaatgaat
    11581 aatttgactt tagaagaata tttacatata aattaagaga tggtttgtta aaattaggtt
    11641 aaaataaaaa ataacctaga ctattagctg tgtaaagtga tataatgtaa atacaattta
    11701 agataactac tcgaagggga gtagcttaca cgaaagtggt aattaatcgt caagacagta
    11761 gaaatactcg gttaattagc aagggattcc ttgatagcaa gaccttcaaa ataagcatta
    11821 ggtatgtttg ttttggggtc tttttttatt caaggaattt ttatttaaaa aaatttaaat
    11881 ttcaagtata cataagagcc cccaaacata aaataaattt attgaagagg agggcaacaa
    11941 atgtcttaca aaagtactta tcaagaagta attaaaaatt tagactcaag tttatctggt
    12001 ttgagtaaag aaagagcaga agagttgtta gaaaaaaatg gagctaatga attaaaagaa
    12061 gcagataagg ttcctactta taagcttttc ctagaaagtt ttaaagaccc attagtaata
    12121 attctattga tagctgcttt agtacaaata tttttaggtg aaacagtgga gtctataata
    12181 atttttgcag ttattattat aaactctgtt ctgggtgttg tacaaacaaa aaaagcagaa
    12241 agttcattag aaagtttgaa aaatttatct gcaccaaacg caaaggtaat aagagacaat
    12301 aaaaaaatga caattccagc aaaaaatcta gtagttggag atattgtatt cttagaagct
    12361 ggcgattaca taccagcaga tggaaggctt atagaagcac aatctctcaa ggttgtagaa
    12421 gggatgttaa ctggagaatc agaaccagtt ttaaagcata tagaaaaaat aagtgaagat
    12481 gtaagtttag gtgaccagaa aaatatggta tttagtggtg ctgtagttgt ttatggtaga
    12541 ggttcttttg tagtgactgg aacaggtatg aatactgaga tgggtaaaat agctgggctt
    12601 ttagaaagtg ctgaaaataa acttacacca ttgcaacaaa aaatagagga ttttagtaaa
    12661 aaacttggtg taggtattgt aatacttgct cttttaatat ttataattca agtagcaaga
    12721 aattacttta tggctggaaa tggagaaatg acacaaatta ttttagattc attcatgttc
    12781 tctgtagcag tagcagtagc agctatacca gaagctctat catcaatagt cactatagta
    12841 ttggctgttg gaacaaatag tatggcaaaa aaacaagcca taataagaaa attaccagca
    12901 gtagaaactt taggttcaac aagtgtaata tgtactgata aaacaggtac tcttacacaa
    12961 aataaaatga cagtagttga ttattatatg tatggaacta ataatgatga gttagataaa
    13021 gaaaaagtaa actattctta tcatgctaaa ttattgacta tggtttctac attgtgtaat
    13081 gactctcata taactagtga tggtaaagaa gttggtgacc caacagaagt ggctttaata
    13141 aattattcaa gtaaaaatga tttagattat gataaattaa gaagtaagta tatcagaata
    13201 aatgaaatac catttgactc agacagaaag cttatgtcta ctgtaaatag tatatatgga
    13261 gattatataa tgtttacaaa aggtgctcca gatattgtgt ttagtagatg taaatatgct
    13321 ttgaaaaatg gttcaaaagt agatataaca gatgagataa tagaagaata taaacataaa
    13381 aatgaagagt tctcaaatag agcacttaga gtattagctt ttgcaataaa agatgtccca
    13441 gaagaagatt ttataccttc aattgatgac gaacatgaca tgacattggt tggaataatg
    13501 gcaatgatag acccaccaag agaagaagta atggatgcag taaaagaggc aaagagtgct
    13561 ggtataaaaa ctgttatgat aactggtgac cataaaacaa cagcagcagc aattgcaaaa
    13621 gagattggta taatggaaga aggagacttg gctcttactg gaaaagagct tgatgctttg
    13681 agtgaagaac aactatatga aaaactagaa aatataagtg tatatgccag agtctcgccg
    13741 gaaaataaaa ttcgtatagt aaaagcatgg caacataaga ataatataac agcaatgact
    13801 ggtgatggtg ttaatgatgc acctgcatta aaacaagcag atataggaat cggtatggga
    13861 agtggtactg atgtagcaaa agatgcttct gctatggtat tggttgatga taactttgca
    13921 agtatagtaa atgctgttga agttggtaga acagtataca gtaatataat aaaatcaatt
    13981 acttatctat ttgcaggaaa cttaggagct atagtatcca tagtatttgc agtgtttgca
    14041 ggttggtcaa atccatttac agctttacaa cttttgttta taaacttagt aaatgactct
    14101 ttaccagcaa ttgcattagg gtttgaaaag tcagaaaaaa atataatgaa taaacctcct
    14161 agacacccgg atgaaagtat actagcagga ggaacattga aggcagttgc tataagaggt
    14221 tgtataatag gtgtagttac aataattgca caatatatag gattacaagt atctccagaa
    14281 cttggagtag caatggcatt ttcaacattg atattagcta gaataatgca aacattacct
    14341 gcacgttcta ataatcaaac agtaataaaa ttaggttttt tcagcaataa atacgttata
    14401 ggagcagttt tagtctgtct tgggctttac tctttaacac taataccagt gctaagagga
    14461 atattctcta taccaacaac gtttggattg taccagttgg ggatttgcct agggttagca
    14521 ttaatttcat caatattaat ggaacttaac aaattaataa atagaagtga agtaaaataa
    14581 tcaaaaaatt aaagggcata ttgattttaa caatatgcct tttataaata gtctaattta
    14641 ttctatatta tcgattattt ggaatgcttt tataacttca ggtaattctt tttcttcagg
    14701 cattctaaat cttataaaat ttttatcaag tccagtaaaa tcacttccag atataactct
    14761 tatttttaat ttagcaaact cttgttgtaa atctatgtcc ttgtttttat gttcaactgt
    14821 cataattgat acagtatcac tagtttcagc tatattaagg tttttccatg gaattaataa
    14881 ttgatttttt atatcttttg ttttttcttt taattcttct ataaattgaa catcatctaa
    14941 taaatttgct gcaatactac gagatatttc agacacttcg taaggagtag aaatcttttt
    15001 aatagggcta acaagttgtt ctggaagaac tgcatatcct gctcttagtc ctgctaaacc
    15061 aaatcctttt gaaaatgttt ttaaagcaat tacattatca taattattta gaagcttgac
    15121 agctgaattt tcttttggca tgtattcacc ataagcttca tctaccataa ctgctatatc
    15181 atatttagct gcttccctta ctatattttc aattgatgat aaaggtataa tttgtcctgt
    15241 aggattgttt ggattgtcta tgtaaattac tttatattct ggatttattt tttcaataaa
    15301 ttctttttca ttaaacttga aattatcctc tttctttaaa agaacataat cataagttgc
    15361 accatgcatt tttatatctg tctcatattc tgagaactga ggtgaatatc ctaaaacttt
    15421 atctcccttt tcaataaata gtctatttaa aaggtaaata acatgtatag aaccatctgc
    15481 caaacaaatt ctgtcagtat ctaatgctat aaaatctttc caataatcga ctatattatc
    15541 ttttaaagca attgaatgag gatatcctct tatcatatca aattttaaag cattaaaagc
    15601 ttctacagct ttgtttgagt aagacacggt atttatacct tcaccacagt ctatctcgaa
    15661 tacatcttct gaatgtccag attctattgc ataacttttt ttcaactgca tttcaatagc
    15721 tttgttaatt tttagtttca tactttatca ctccttattt agtcaataat aaatgttata
    15781 aaagttgcta gatttaaaat attatgaaaa tatataatag taaaaatgaa tataaatatt
    15841 ataatagctc actttattta atatgattat atatcatata ttgtaaaatt tctagcatta
    15901 taagactata aattgaaaat attcagaaaa atactacaag aaacgattta ttttgctctt
    15961 aaaataagtt aaatgaatgt tattaatttt gtaaattgaa atagtttaag tataaagtta
    16021 tgatataatc taataagggt aattctttag attatattaa ggaaatatag gtatacaata
    16081 aaaagttatg gagttggatt atgggatata aattaattgt aacagatatg gatggaactt
    16141 tattaggcaa taatcataaa gttacagatg aaaataaaac tgcactacaa aaagttataa
    16201 aaagcggtat taatgtaact ttagcaacag gaagagcctt cgattcagct aaatgcaatg
    16261 tagattttct taaagaggac atgccaataa tagcttgtaa tggttcatta ataagagagc
    16321 aaaatggaaa tataatatat tcaaataaga ttgatacaag aacatgttta aacattttag
    16381 atgtattaga taaatacgat atttactacc aatgtaatag tatagattct atgctttcga
    16441 aaaaaataga aggacgtgaa gatagattaa gtgtattttt aggctctgaa acagaagtaa
    16501 ttgtaaaaga tgatttgaga gaagaaatct ttaaaaaaga tatactaaaa tttgtaatca
    16561 tagaagaaaa aaatccatct attttggatg aaataagaaa agaattgaga aaagttcaag
    16621 gaataaagat aacctcttca tggccaaaca atattgaagt catgaatgaa ggtgttgata
    16681 aaggtaatgc agttaaaatt ttagctgaaa aaatgaatat agacagagaa gatataattg
    16741 catttggaga caattacaat gacatagaaa tgataaagtt tgctggtctt ggggtagcta
    16801 tgggaaatgc agaagaattg attaaacaag aagctgatta tgttacagat acaaatcaag
    16861 acagtggagt tgcaaaggca atttataaat tcctagagtt aaaagaatca cttagctctt
    16921 aaaacagtat ataataggaa aaaataggtt gactattttg tagatagtaa ttataataca
    16981 aatagataaa atataaaaat ttagattcag tgaaaaagag agtagatata gagaaaatct
    17041 cagcgaattg ggggatggtg tgagcctaat gataacactt tatattgaaa agaactttgg
    17101 agaatactac ttgaaattca gtatggtagt aggtaatctc gccttaagag aatagagtgg
    17161 gtaaacattg tttaccaact agagtggtaa cacggaatat ttcgtctctt ggtctttttg
    17221 accaagagat tttttagttt cattgagtta caagacttat aattaaaata caaaaaatta
    17281 aaatatgagg tgaaagttat gaaattatgg ggtggacgct ttagaaaagc ggaaaatcaa
    17341 cttatggaag aatttaataa atcttttggc tatgattgtg tactgtacaa aaaagatatc
    17401 gaaggaagtg tagcacatgt tcatatgcaa gtaaaatgtg gacttttaac agaagaagaa
    17461 ggaaagtcta taacagaagg tttaaaaggg atacttgaag atgtagaaaa tggaaagtta
    17521 gtattggatg gagagtatga agatatacat agttttactg agataaattt aattcaaaga
    17581 attggagatg ttggcaagaa gcttcataca gcacgtagta gaaatgacca agttgcagtt
    17641 gatatgagat tgtatgctaa agaaaaagca aatgatttgg tggatttaat atctgaattt
    17701 aaagctacta taaaagatgt tgctgacaaa aacccagtga tgatgccagg ctatactcat
    17761 cttcaaaggg cacaagtagt gacatttaag catcacttaa tggcatatta tagtatgttt
    17821 gatagagatg agaaaagaat aaaaaatgct atagaaatat tagatgaatc acctttaggc
    17881 tgtggagctt tagcaggaac tacgcatgat atagatagaa gtataacttg tgaacaatta
    17941 ggatttaaaa aagtagtaga taattttatg gatggagtaa gtgatagaga ttatttacta
    18001 gagctaatga gtgatttttc aattataatg atgcacttaa gtagattaag tgaagagttg
    18061 atactttgga gttctcaaga atttggattt gttgagatag atgatttata tacaactgga
    18121 agtagtataa tgcctcagaa gaaaaatcca gatggtgcag agcttataag aggtaagact
    18181 ggtagagtat atggaaactt atttggtctt tttacagtaa tgaaaggaat accattagca
    18241 tataataaag acatgcaaga agataaagaa ggttttttcg atagtgtaca tactcttgaa
    18301 atgtgtatac aaataatgga tagaatgata gccacactaa aggtaaatga ggataaaatg
    18361 aaacaagcag taaaaaatgg ttttttaaat gcaacagaag ttgcggatta tttggttaaa
    18421 aataatgttg cttttagaga tgcacatggt atagtaggaa gtatcgttat atattgtgaa
    18481 gataataaaa aagcaataga agatttaaca ttagaagaat tacaaaagtt ttcagatgct
    18541 tttaaggaag atatttatga ttttatcgac tatgagagta tattaaataa gggaataaaa
    18601 aagaatttaa aataatgtat ttatttttaa aagctgtaaa tttgaatgtt atcatttaaa
    18661 tttacagctt tttattgtgt aagattttat tatataaaag taagaaactt aaaaggagta
    18721 gtatttaaaa taaatacaaa taaaaaatta ttattaaaat gataattaat atcaataagt
    18781 attgacaaaa ttatttctaa atactattat atataatata aataataatg aaaatcaata
    18841 tcaaataaat gggaggaaat ctaatgaact tattagtgat tggtggtcat gaaagaatgg
    18901 aaaaagatta tatggttatg gccaagaaaa aaggttataa gacaaaggta tatactacca
    18961 tgtcatctaa attaaataat tctataggaa aaccagatgc agttgtcata cttacatcaa
    19021 ctgtttcaca taagatgtct agaacggttg agtctcaagc taaaaaacaa gatatattaa
    19081 tcgtaaggca taaaaatagt agtaaggtag catttaatga atgtctagat atggttgatg
    19141 aatgtctggg aaattgttca aaatgtaagc ttaaagacaa acaaaaacaa aaaaatatat
    19201 aggtataaaa aatacataaa cataaaatta aaagtactga gataatagtt ataattatta
    19261 tctcagtact tttagattta taaggataag tatagtataa attttttatt ataatttcca
    19321 actacttgtt actttttcat atttttttta cttctagctt tttttctttt tttcttcttt
    19381 atatttgaag ctattataaa taaaactaaa agcactagaa tacctgcaat aatcttaaca
    19441 aaagtcatta aaacattatt ttcagtcaaa aatggaagac tgctatcaag attatttttt
    19501 gcaattaagt ctatactgtt ttctagttta ccattattat atacatttaa agtaccaacc
    19561 ttagctcctt tttttatagg caaatcaatt ttatttaact ctgttttggt atcataattg
    19621 ccttgagatt catctttttc taagaatatc ctataactat tttttggttc ataaatcaat
    19681 tcatgttgtt ttgtaaacaa tactttttta gtatctgtgt atttttcttt atcaacaata
    19741 gtagcacact taaagttatc aaatccataa tcaatcagaa tacgtgagtc taaatataaa
    19801 tcagcattag ttgaattaaa tacagcaacg ataactcttc tgccatcttt tacagcactc
    19861 gataaaagac atttaccagc agcatcagta tatccagttt ttataccatc aactatatca
    19921 tattttatat ttataggttg acctttgtat gtaatcttat catgtgatgt caaaaactta
    19981 ttagtgttta caaagtatct ctcataagga tatgcttttg tagcttcaaa tttaagtgac
    20041 ttagttttta caatttgtct aaaagtatca ttattcatag cttctctagc tatgagagcc
    20101 atatcatgag ctgttgtgta gtgatttggg tctggtaatc catgaggatt attaaaatga
    20161 gtattagtag ctccaatttt ttttgcctca gagttcatga ggtcaataaa tttttgtaca
    20221 ttgccaccac caacatatct agctaataca aaagcagcat cattagcaga atgaaccaaa
    20281 agagcatcta gtaactgctt tacagtaaaa gattccccat tttttagata catactagag
    20341 ccatctattt gaggtaaatc tttaatttct attacttgat ttaaatcttt aacctccttt
    20401 agaactaaac aagctgtcca tactttagtt gttgaagctg gataaagctt agaatttcca
    20461 tccttattat atagtatttt gccagactca tagtccatta aaactgcatg tttagcaact
    20521 atagctggtt cattatctgc aaaagagata ttagagcttc ctaaaaatga agtaaatatc
    20581 aataaacaga ttaataaaag tgataaattt cttttcattg tgagcctcca aattttaaat
    20641 tcatctttta gtatagcata tatttgtcaa taattaaaag aaaggagtga ttaatatatg
    20701 aaaaaaaagg taaaatttat ttcgtttttt gttttattta ttttaataat atcatcaata
    20761 atcgtttata aatcgcttat tttcaaagat gatatttatg ttgtaagtaa aaaaaattta
    20821 cctaatgaaa aaacacatgt tgtagagaat gaaattaaag gtgaagataa taatcacagt
    20881 aaagtaacta aagacataga taaaaatttt caaaataata aaataactat atacataagt
    20941 ggagcagtaa atagacctgg aattgtaact atagagtcag acaagagact ttatgatgct
    21001 gtagaattac taggaggaac gacaaaagaa gcagatttaa atggagtcaa tctatcagta
    21061 agattagaag atgaacaaca ttacataata cctaaaatag gtgaagcaac atctgttaca
    21121 agtaatgatg attccaataa actaaatcag aagaatgagt caagaggtga gcctaaaaat
    21181 aaatccaata aaaatatatc taacgaatca aaagtaaata taaatgtagc gacgatagaa
    21241 gagttggatt ctctacctgg ggttggagaa gctacagcca ataaaatttt acaacacaga
    21301 gaagaaaatg gacagtttag ttcaattgag gaaataaaaa atgtaaatgg tattggagat
    21361 aaaaagtatg aaaatataaa agatttaatt tgtgtagact aaaatataat acaaaactta
    21421 catatagata tgttattata aatatgtatg tatatgtgtg tacatgtgga aatttagtaa
    21481 aggaggcact tttatgaaaa aagaacaaaa aaaacttaca gagatgacaa cagcaggtgg
    21541 ttgagccgct aaaatagggc cagaggttct ggcatcagtt ttatctcaat tgcctaaaaa
    21601 tgataatata gaaaatttat tagtaggact agatacagca gatgatgctg cagtttataa
    21661 attaaatgat gatatggcac ttatccaaac tttagacttc tttacaccaa tggtagatga
    21721 cccatacgta tttggtcaaa ttgctgcatc aaattcacta tcagatgtat atgctatggg
    21781 aggcaaacct ttggttgcta tgaatattgt ttgttttcca tcatgccatg atatggatgt
    21841 acttgcagaa atattaaaag gtgggtttga taaagtgaaa gaaagtggag cacttttggt
    21901 tggagggcat actgtagatg acaaagagcc taaatatggt ctttcagttt ctgggattgt
    21961 aagtccaaat aaagttttat caaatgctac tgcaaaacct ggtgataagt taattataac
    22021 aaaaccaatt ggagtgggtg tattaaatac agccatgaaa gaaggtatgg tagaacaaca
    22081 tatagcagat aaagtaatag aaataatgat acatttaaat aaatatgcag caatgagttt
    22141 tgagaaattt gatgtaaatt cggttacaga tataactggt tttggtctac ttggtcatac
    22201 tttagaaatg gccaaagctt ctgaagtaag tattgaaatt gaatcaaaac atgtaccaat
    22261 tattgaaggt gcaatagaaa tggcacaaat gggaattata cctgctggta tgtataaaaa
    22321 tatgcattat gtatctaaag atgtagaagt tgtaggaaat atagaagttg cagttcaaga
    22381 tattttatac gacccacaaa catcaggagg gttgttaatt tcagtaaaag aagaacttgc
    22441 agaggagctt gtaaaagata tgaaattaaa tggagcaata gaagctaaaa ttataggttc
    22501 tgtagttcca aaaggggaaa aatatattac agttttataa aatattatag agaaagagtg
    22561 attgttttga gcaaaagaga attgtttgca atgttgcctt cagttgatga agtattaagt
    22621 gataatagaa ttgtagaaat aataaatgaa tatcctagaa gtttagtttt agaatctgtg
    22681 agagaagtaa tagatttaaa aagacaattt atattaagat taaaagaaga tgcatcaaac
    22741 tctgtaacaa tagagtttgg tgaaataata gaatctgcaa tagagagagt taagttaaat
    22801 tactcattat ctttaaaaaa agttataaat gcaactggta cagttattca tacaaattta
    22861 ggaaggtctt tacttagtga agatataaaa gatgaattat ggtgtgctgc ttcaaggtat
    22921 tcaaacttag aatacgattt ggataatgga gaaagaggtt caagatactc tcatttaact
    22981 agtacaataa aaagacttac tggagcagaa gatgtactag ttgtaaataa taatgcagcg
    23041 gcagtacttc ttgtacttag cacaatggct aaaggaggag aagcaattgt atctcgtgga
    23101 gaattagttg aagtaggtgg ttcatttagg attccaagta taatggctct tagtggtgca
    23161 gaattagttg aagttggttc aactaataaa actcatttga aagattacaa ggaggccatt
    23221 acagaagata caaatgtatt gatgaaggtt catacaagta attatagaat aatgggattc
    23281 acagagagtg tttctataga agaattggtt aatttaggta aaaaatataa actacctgta
    23341 atagaagact taggaagtgg agtttttata gatttatcaa aatatggttt atcttatgaa
    23401 ccaactgtat tggattctat tcgacaaggt gcagatgtag taacttttag tggagataag
    23461 atgcttggag gtccgcaagc aggtattata gttgggaaaa aagagtatat agaaaaaatg
    23521 aagaaaaatc aacttactag agcacttaga gttgataagc ttacaatatg tgctcttgaa
    23581 gcaacattga gaatgtattt agatgagaca aaagctatag aaaatatacc tactttaaaa
    23641 atgttgacat ataaaattga agaattagaa gtaaaagcaa ataaactatt tgaaaaaata
    23701 actgctttaa acttaaatgc aaacataaat atagaagatg gattttcaca ggtgggagga
    23761 gggtctatgc ctcttgaaac tataagtaca aaggtaatat caataactcc tgaacatatg
    23821 aatgtttcat ctttagaaaa aaaactaaga cttagtgaag cacatataat agccagagtg
    23881 tatgataata aatatgtact agatgtaaga actatatttg atgatgaatt tgatgtaata
    23941 gttgaggaat tgagaaaagc atttaattaa ataaatttga gttaataaat attaaattta
    24001 gaagtataag tatgaagttt tgattaaaaa atagctacac acacttgtga ctttagtcat
    24061 agcaggttta gagggggcaa aaaatgaaaa atgtagtaat tggaacagca ggtcatattg
    24121 accacggaaa aactactctt ataaaagcat tgactggaag agagactgat acgttagatg
    24181 aagaaaaaaa gagaggtatt tcaataaact taggatttac ttattttgat ttaccaaaca
    24241 atagaagagc tggaattgtt gatgtcccag gacacgaaaa atttataaaa aacatgttag
    24301 ctggagcatc aggaattgat atagttttac ttgttgtagc agcagatgaa ggggttatgc
    24361 cacagacagt tgagcattta gatattctaa gctttttaaa tatcaaaaat ggaatagtgg
    24421 ttctaactaa gtctgatact gtagatgatg aatttagaga gctagtaaaa gaagatattt
    24481 gtgaaaaaat aaaaggaact tttcttgaag atgctgaaat aattgaagtt gattctatat
    24541 ctaaaagagg tatagacact cttattgaaa aaatagataa aatgagtgat gatatagaag
    24601 ataaaaatga aaactctcca gcaagactta atatagacag agtattttca ataaaagggt
    24661 ttggtactgt aataacaggc actttaatag aaggaaagat aagtatagaa gatgatttgg
    24721 ttatatatcc acaggtatta aaaacaaaaa taagaagtat acaagttcat ggagaaaata
    24781 aagatacagc ttatgcaggt caaagaacag caatcaatat atcaaatgta aaagtggatg
    24841 atataaaaag aggagatgtt ttagctgcac caaactcatt ggaggaatca atgatgttag
    24901 atgtaaaatt atcattggta aatcatttag ataaaggatt aaagcactgg gatagattaa
    24961 gattgtatca tggaactaga gagatacttt gtagagcagt tccactagat aaagaattaa
    25021 ttgaaagtgg agaaagtggc tatgttcagt tgagacttga agaaagcata gtatctaaga
    25081 aaggtgatac gtttgtagtg agacgttatt ctccaatgga aactataggt ggaggtgtaa
    25141 taattgaccc atctcctaaa aaacataaga aatttgatga aaaagttata gaagcattaa
    25201 aaataaaaga aaaaggcgaa ctaaaagata taatagaaga gtacttaaaa aggaatttaa
    25261 aaaattatcc aaatataaaa gaaataatga gctatagtgg tgctcatgaa gaagatgtaa
    25321 aaagagcgct tgaaacactt atatcagaag ataaagtatt tattatagga aatatgtata
    25381 tgcatataaa tcaatacaat aaattaaaag aaaatacaat aaagctttta agtgagtacc
    25441 ataagaagta taggcttaga aaagggatac taaaagaaga agtaaggtct aagatagaga
    25501 gtaattttaa gactagggag atggatattt tattagaaaa attgtctaca gaaaatgcta
    25561 taaaaataga aaataatata gtatctctat tagattttga ggttatttta aatgataagc
    25621 aaaaagaaat agcaaaaaaa atagaaaaaa gattaaaatc atgtggagtt tcttctatac
    25681 tgacaataga tgaagtttct gaaggaaatc ataactatgc tgaagtattg gagtcaatga
    25741 taggtaataa agtagagaaa ttagatgatt tgtatataat ggacaaagat atttatgaaa
    25801 atgcaaagaa cattttgata aattatataa aagaaaataa agaaataaca ttaggagaat
    25861 acagagattt gattgattct agcagaaaga attgcatgat tatattggaa aacttcgata
    25921 gaaacaaaat aactaaaaga gtagaaaaca aaagaatttt gttttagcag aaaatgtctg
    25981 tttttgcttt aaggtataaa gaaggaatta taatgtcgag tatcgtataa ttaattagta
    26041 taaaatatta attaagagga agtgtaaagt attgaagaat ataatacctg atgtaaaacc
    26101 agatataaaa gaaagagaag acatgagcct tttatttata ataatgggct cagtactttt
    26161 attatttatt ataaaagata tagagaaata tgaaacttta ttacatgcca tatatacgat
    26221 atttttagta gctacaggag taattatatt taatactaca aaatttaaag tagattcatt
    26281 ttctacattt ttaggattaa tttttatagc tactggagta ttggagtgta tatatttatt
    26341 taattccttg gggattaaaa ctaagtctat aatggagatt aacatcacaa tatcagcaat
    26401 aacagattta tttcctatac tgggtgtata cttaagtttt aaatttgtta aggataacaa
    26461 acaaatttat tcaagtgtaa ttttatttat agcaactgct acattaacaa taacatcttt
    26521 attcataata tcaggaattt gtgactatct gggaaatggt aaatcacaat atgctttagg
    26581 agttataata tctatattca taataattat ttctatcatt agtggaattg aattaaattc
    26641 aagttcaaat aaaggtaaat gggaatatga tgaaaaaaag atttttaaca gaataatagt
    26701 aataataatc ttatcaagag ttccaaatct attgcatata gttattgata atagaaatgt
    26761 agagaggata ttatcacaaa taataataaa tgtagcctta tattatttat ataattacat
    26821 agtatctaag aatattaaga aaacagcact ggaattgcat gatacgaatg aagaattaac
    26881 aaaaaaaacg gaaagtttaa aagaaaagaa taaaaaatta atctatgaga cagaaaagat
    26941 agaggaactt aagggaatac ttatgaaaag agaatctagg ctacaatcta cattagatgt
    27001 agcagtaaat agcatagtag tatttagcaa agatggagaa attacatatg caaataagag
    27061 ctttagaaat acttttggag actataaaga acaagataaa ttgactgata aaataaaaaa
    27121 cttcaatgat ttgatagaaa gcattcatta tgtgtttgtt catgagaaga atgcagagaa
    27181 acttattagt acaagcgata ataaagttta tcaagctata tttgcacctt taattattgc
    27241 ttcacaaaca gaaggagtat tgtgtgtact agaagataag actaagaaaa aggagtacga
    27301 acgcaaactt attgaagcaa ataagagaag taaagatttt ttagaaagta ttggagatgg
    27361 catagtagtg cttgaaggta ataacaaaat atatgttaat aatgcatgta gggaaatttt
    27421 taaaaataat ttagagagta ttgatttttc attagtatgt aaaagtgaag aaagtataga
    27481 aaaaagatat gtaatagatg gtgaagtaaa gtatgtagag atgagttttt ctcagtatac
    27541 taatgctggt actaataaaa ctattattgt aatacgtgat acaacctctc gaaaaatatc
    27601 tcaaataaaa cttgaggaaa gtcaatcatc atattcaaga tttatagata tattacctga
    27661 tggcatatgc ttattaaaag aagatttaag tataaactat gcaaataagt cgtttttaga
    27721 tatgctagct tttacaaaca tagatgatat aaaagattct aatattaaac tattaatgaa
    27781 tgcgaatact gaagaaaaaa tgaaatttac ggataatatg gctaaagttt taaatgaaaa
    27841 tagaagtata cttttactgg aacatgaatt gataaatagt tatgatgata tagtagaagt
    27901 agaattaagt gcattacctt ttgctatata caatactaga tacataatgc ttattataaa
    27961 agatttagta cataagaaat attccgagca agcagaaaaa gaactgcttg aaagatttaa
    28021 aacagataaa atcaaaacgg agttttttgc aaatatgtct catgaactaa aaactccact
    28081 aaatgttata tctagttcaa atcaactggt agattctttc tatagaaatg aaaagataaa
    28141 agattacaat aacaatatta aatctcatgt ggatttagtt agacaaagtt cttataggct
    28201 tcaaagatta ataaataaca ttatagattt aacaaagatg gagtctgggt tctatacact
    28261 aaaacttgcg aaatacaata ttgtatctgt aatagaagac ttatttatga atatagaaga
    28321 gtatgcactt agaaaagaca taaaaattct ttttgataca gaccttgaag aaattaatgt
    28381 ttatatagac aaggttgaaa ttgagagaat tatgttgaat ctattgtcaa actgtattaa
    28441 gtttactgat aatggaggct ggatatatgt aagtattcat tataaaatag ataaagtaat
    28501 cataagtgta aaagacactg gagttggaat accacaagat aaattagagt tgatttttga
    28561 agaattttca caagttgata agactttatc cagaaatact gaaggaagtg gaatagggct
    28621 tgctatagtt aagaatctag taagtcttca tggtggagat atagaagtag taagtgaagt
    28681 gaataaaggc acagagttct taatttcact tccaatgaag ggatttagta gtgaacatta
    28741 cacagaagat aagagaatat ataatattca agaaaaaata aaaatagaat tttctgatat
    28801 atattattaa aaatgattat gagcaaaaat aaaataaaaa tactatttgt ttaaatattt
    28861 aagttgtaag aaataggcta tcataaggta aattattatg atagcctatt tttagctagg
    28921 ttttatatct atatgtgaga acatataatc tctaaattac cttcaaaact aaaatctcca
    28981 aagtcgtttg gcagtataaa atgcataccc ttttttaaat tatattctac agaattgtgt
    29041 ataagttttc catttccatc caacacactg tataaagaaa aaggtgtatt gtgtgtaaaa
    29101 ttacattttc caaaaacatc taatttataa acactaaaaa attcgtttga cacaaatgtt
    29161 gtgcatttaa agtctgatgt tgatacgatt ttatagtcag tatcgaaatt tatatgagga
    29221 acattcgtaa catctattga tttttgaaca tgaagttcac gcttattacc tgaattgtca
    29281 gttctatcat aatcatagac tctataagta gtatctgagt tttgttgagt ttctaaaatc
    29341 aatgttcctt tgcatatagc atgtatagtg ccacttggaa catagaaaaa gtctcccttt
    29401 tttatattaa cctttctaag tagtttgtcc cattcattat tatttaccat atttacaaat
    29461 tctttatgag attttgcatt atgacctatt ataatttctg catcatcaga acaatctatt
    29521 acataccaac actctgtctt acctaactct ccattttcat tttttttagc atattcatca
    29581 tttggatgaa cttgaactga taaattatca tttgcatcca atatttttgt aagtaaagga
    29641 aacttatcac caggtgtgtt tccaaataat tctctatttt tattccacaa ttcagatagt
    29701 tttttacctt tatattttcc attttctatt aagcaatctc catttttatg agagcttata
    29761 gcccaacatt caccagttgt tggcgaatct atttcataat taaatttatc tttaagtgca
    29821 gtaccacccc atattctatc cataaaaata ggttttaaaa ataatggttc cataatcctg
    29881 ttcctcctat gatattaaat ttatcctaca aataattata tataaataga ttataaaatc
    29941 aatagtaaaa aagttttgtc ccttgaaatg acataaagta taaggagtta ttaaataaga
    30001 taaactatga tatataatat aaagttctaa attggagtga taattatgga gttgtttaag
    30061 gcagaaagca aaaaatataa aaaagaattt ttaaaattat tggagcaatg ggttagtata
    30121 ccttcatttt atgatagaaa aactgtatct aaagacatgc cttttggaaa aggagtatat
    30181 gatgcattaa actggtttga aaatctaggt agagaaaaca atttcaaagt aaaaaatata
    30241 gataatcatg ctgttcaaat tgaatatgga aatggtaaag agtatgtaga tatatttgga
    30301 cattgtgatg ttgtaaatcc aggtgaagga tgggatagtg aaccatttaa actaaatatt
    30361 ataggtgata aattagttgc tagaggtgta tctgataata aagggcctat gatagtgaat
    30421 tttttagcac taaaaatgat aaaggattta gatattaatt taaaaagaaa ggttagatta
    30481 atagctgggg gaaatgaaga gagtggattt aaatgtataa agcattatta tagcaaagag
    30541 ccatatggtg tatgtggatt tactccagat gctaaatttc ctgtattaaa tggagaaaaa
    30601 ggtggagcaa taataaagtt aatttcaaat atagacgata aatctttata tataagtggt
    30661 ggaatagagt ttaatacaat accagataaa gtttatataa agaatgtaga aaaattgggt
    30721 aaagataata tatgttttga tattaacaat atatcgataa attatgataa tggcaattat
    30781 attgtgtatg gaaaaggtgg tcattcatct aaacccgaaa aatcaataaa tcctatatta
    30841 gctacaatta aacttttgtc agaaaatatt gatgaaaaat ggacaaaaga tttatataag
    30901 ttgataaatc aagataatat aaatggaaac ttatttggtt taaatattga ggggaaatgt
    30961 ggaattttat caatggtgcc aacaataata aatatagtag atggaaaatt agaagtagtt
    31021 ttaagcgtta gatatccaga aatattaacc attgaagata taataaaaaa atttaatttg
    31081 tatatggagc aaaataatat taataaattt gagcttattg gggaaaattt aaagcaagca
    31141 aattatatag atagaaattc aaaacttgta agaagtttgc atgatatata tataaagtat
    31201 tctggagatt taaaaaatga tgttagagta actagtgcag gaagttatgc ttctgaaatg
    31261 aataattcag taatatttgg ttgtgaattt cctgatggta gctttggcaa tgtccatagt
    31321 gcgaatgaat ttgctagctt agatagattt ataactgcaa taggtatata tgcagaggca
    31381 ataattacat tatgtaataa aatataaata caaaatgtcc cttatataga catattatat
    31441 atacaattaa aattttactt gatatataat tgaaatcaaa gagaaggtga tacaatgata
    31501 actaagaaac atgcaataat agtaaagagt ttaagcaata aagatggata tatgacatca
    31561 aacgaattag ctatcaaatt ggatgtgtct actaaaacta ttaaaagata tatagcagat
    31621 ttaaatagtg ttttaagcaa atatgatttg gtaatagctt cttctagagg tataggatac
    31681 aagttaagcg gttctaaaaa caatatagcc agagctgtta aagaagcaaa taaatatatt
    31741 gatggttttt tagatgactc tgaagagtca agaatgtcaa atataatatg tatgcttatt
    31801 aatagaaatt atatgagtat agaagcaatg gcagaagaat taaatctaag tatagccgct
    31861 ataaataaat tgtctagtaa gcttaaaaga aaacttgaaa agtatgactt agttataaaa
    31921 tctaaacctt actatggaag ctatatacat ggtgaagaaa ttaatataag gcaattaatt
    31981 actgactatg caataaaatt agatgaaaaa agtaaagtga aggtgttttt agatgacatt
    32041 agtgaaaacg atatacactg tatagaaagt attttagaaa agcacttaag agaaaaagat
    32101 acaataattt cagataaaga ttttaatctt ttgctttcaa aaataatcgt ttctgtattt
    32161 aggagtaaga gaggtcatag taacaatata aacttaatgg atacaagtta tagatttcat
    32221 aactactact ttatagaaaa tctaatgaaa gaaatatctg ataaaattgg atttaaactt
    32281 attgaagatg aagtaattta tatttcaaat tattctggtg taatagccta taaaggtaca
    32341 caaggaagaa aaaacacaag tgaaattgag gaacgaataa gtagtgttat aagtagtgca
    32401 cttcaagata tattccttat ttcaggtagt gattatacaa aagatgatga gtttatggtt
    32461 gcaatctctg accatataaa gagattttta aatagagcta gagctaatgt aaaatcaaat
    32521 aatcctttac ttcatcaaat aaaagagaag tttcctatag cttttaattt agccgtgttt
    32581 ttatcaaata aactagaaac tgagtttaat ttaaaacttg atgaagatga gcttggatat
    32641 atagctatcc actttgctgc ttcaaatgaa agaatgaaaa aaaatactag taagaaaatt
    32701 tgtatagttt gtcattatgg gataggaaca ggacaactta tttctgaaaa gctaaaacag
    32761 aatataagtg atttaagtgt tgtaggtgtt tatccagtta gatatctcga tatggctata
    32821 agtcaagatg ttgatttgat agtatctacc gttgaattaa aaggatatga aaaacctgtc
    32881 ttatatatag aaaatatatt tgatgatagc ttaattgaaa acgtaaacaa agccttttat
    32941 gaaaaggaag aaagaagaaa gataataagc aacatgtttg atgaaaaagc ttttttcagt
    33001 ataaaggcta atactaaaga agaggtcata atttcacttt caaataaact aaaagaaaga
    33061 gattttatag aggaaagttc tataaaaagt ataatagata gagaaaatat ttcatcaact
    33121 gaaataggaa atctagtagc aataccccac acaatagtga agggagataa aaaatctata
    33181 ataggagttg gaatacttga aaatccaata atttgggata agcaagaggt tcagctagta
    33241 tttatggtat tttttaatac taaagaaaaa cataatttct cgatatttaa atacctttat
    33301 aattttataa aagatgaagg cggagtaaga ggaattatta aaatatgtga ctttaataaa
    33361 ttaatggcac tgataggtaa ttaaggagga aattaataat gggaacaaac atatttaaca
    33421 aagaatatgt atttttaaat gttgatgcta agtcaaaggt tgaagtttta aaatttatat
    33481 caaaaaaagc taaagactta aacttggcag aagatgagag cttagtatat gaaggtttaa
    33541 tggctagaga agaacaattt actacaaatt taggagaatc tatagcaata cctcacacta
    33601 aaaatgatgc aattgaaaat ccagcagtag tagtattgaa gtttaatgaa gatgttgttt
    33661 ggaatgaagg tgaggataag gttaaattag caataagctt acttatgcca ggaaaaagta
    33721 aagagaatat tcatcttaaa ttattatcat ctctttcaag aaaattaata aataaagaat
    33781 ttaaagattc tcttttaaaa agtgataatg ttgaagaaat atctaactta ataaatgaag
    33841 ctttaggttt ataattaaac actaaggtta taaaataaaa aaataattaa ctaaaaatag
    33901 gaggaatttg aaatgaataa aaaattagta gcattatgtg cttgtccaat gggactagct
    33961 cacactttca tggcagcaga agcaatagag caagcagcaa aggcattagg ttatgaagca
    34021 aaagttgaaa ctcaaggtgc tgatggtgta caaaatgagt taacaagaga tgatatatta
    34081 ggagcaacta tgataataca tgcagtagca ataacaccag aaggtatgga aagatttgat
    34141 ggttgtgaag tttatgaagt tgaattacaa gaagctataa aaaatgcaga aggtgttata
    34201 aaagaaatag aagaagattt aggaatataa taaatttatt gtggaggaaa tagaatggca
    34261 attaagaaaa aagttataaa cagttcaggt gcttctacaa caggaggaaa taatgtagga
    34321 aaatctactc catctagtcc aactcaatca aagggaaatg gaaaatggaa agaaataagt
    34381 aaacatataa tgacaggtat atcatatatg ataccagttt tagtaatggg tggacttata
    34441 ggggccttat ctcaattaat accatatgct atattaggac ttgacccatc tgtaggtata
    34501 gttgatgcta tgaactcagg agaatttaca gggttcaagc ttagtctttt aaatatagca
    34561 caattaatgt caaatttcgg atttactttg tttggatttg cgataccact gtttgcagca
    34621 ttttgtgcaa actctatagg tggaaaaaca gctttaattg caggatttat aggtggatat
    34681 atagctaata aacctgtagg tgtaccacaa ttcgttgatg gacaatggac tgaagttgtt
    34741 ccagttgcat caggattttt aggtgcaata ctaatagcat ttataatagg atattttgta
    34801 aaatggttaa ataaatctat aaaagtttct cataactggt tagcttttaa aacaacattt
    34861 ttaataccat taatagcgtc attagcttgt atggtattga tgatatttat aataactcca
    34921 tttggtggat taattaatga aagtatgaaa aacttcttaa cagcagcagg agcagctgga
    34981 gaatatgtat atgcaacagc tttggcagca gctacagcat tcgacttagg aggaccaata
    35041 aataaagcag caggattcgt tgcacttggt ttaactacag agaatgtact tccaataaca
    35101 gcaagaacaa tagctatagt tgttcctcca attggattag ggttaacaac attattagat
    35161 aaaagattag taggaagaag agtatatgat agacaattct atcaagcagg aaaaacttct
    35221 atattcttat catttatggg tatatctgaa ggagcaatac catttgcact tgagagacca
    35281 ggttttgtta tacctttaaa catagttgga agtgtaatag gagctattac aggtataatt
    35341 ctaggagcaa ttcaatggtt cccagagtca gctatatggg catggcctct agtagataac
    35401 ttatttggat atataatagg tatagcagtt ggtgctatat tcatagcagt aggaaatata
    35461 ttctatagaa ataaattaat aaaagatggt aaacttgttg tagattatat cgactagtat
    35521 ttaattttta ctatctataa ataactttgg ctggtaatat ggttttaatc aatattacca
    35581 gccttaccta tatgattaat taaaggcggt ggagataaga tatggataag atagaaaaag
    35641 taagagagta ttttagagaa tatgatatag atggatttct tataaatagt cctacaaaca
    35701 agttttatgt tggaaattta tttagctctt caggttatgt ttttataacg aaagaatcac
    35761 aatatataat agtagatttt agatattttg aagaaattaa aagaaaatct agtttgttta
    35821 atgtagtatt aatggacaaa actagaactc attttgatat aattaatgat atttgtagag
    35881 agcagaatat aaaggaaatt ggatttgagg gaaatgaagt atcttttgat ttatatagaa
    35941 gtatgagtaa taagttaagt gcaaccttaa aatcagttga tttatctaca ttaagggaaa
    36001 caaaaaatga agacgaaata aaatatataa aaaaagcttg tgaaatagtt gatgctacat
    36061 tttatcatat agtagacttt ataaaggttg gtatgactga aaagcaagtt gaaaatgaaa
    36121 ttgttagaat tataaaagat ttaggtggtc aaaaagaatc tttcgataca atagttgcat
    36181 ctgggcttag aggagcatta cctcatggaa aagcaagtga aaaagttata gagtatggag
    36241 attttgttac ctttgacttt ggtgctaagt ataataatta ttgttcagat attaccagaa
    36301 ccatttgtat gggtactata aataaagagt tagaagaaat atataatata gtaagaaaag
    36361 ctaatgaaga gtgtataagg gttttaagac cgggaatgac tactggagag atagataaag
    36421 ttgcaagaga cataattggt tcatatggat atgctggtaa ttttggtcat aatctgggtc
    36481 atggtgtagg aattatggtt catgaatatc ctgcattagc tcctgaatca aatgaagtat
    36541 taaaagaagg aatgattgta actatagagc ctggtatata tgttccaagt cttggaggcg
    36601 taagaataga agatgatgta cttataacac aagatggatg tatgagattg actacatcta
    36661 ctaaggattt aatagttgta agctaaaaat aaattagata aatattttat aacaataaga
    36721 aaagaagagg taaatttaaa tgtataattt tgatgataaa ccagatagag taagtgaaaa
    36781 gtgtagaaaa tgggatttaa atattataag agataaattt ggagatatta gagaagattt
    36841 tataccaatg tggattgctg atatggattt taaaatacca acagaaatag aaaacaaatt
    36901 tattgaagct gtaagaagag gtgtatttgg atatacatac tgttatgatg aattttatga
    36961 ttcagttatt ggttggcaaa aaaatatgca tgaggtaaat gtagaaaaag aatggattac
    37021 attaacttat ggaactgtat caacacttca ttatgtagta caagcttttt gtaagaaagg
    37081 tgatagtata atattaaata caccagttta tgaccccttt gaatcatcag caaaaaaaca
    37141 aggtgtaaat gtaatttgta atacattaga tgttgtaaat gatagatatt atataaattt
    37201 tgataaatta gaagttcaaa ttaaagaaaa taagcctaaa ttaatgatgt tttgtacacc
    37261 acataatcca tctggaagga tatggacaat tgaagaaatg acaagggtag caactatatg
    37321 taaagaaaat aatgttattt tggttgcaga tgaagtacat gcagagcata ttcattatgg
    37381 tacatttaat tcaatactta agattggaaa agagctttta gaaaatgtta ttctattgac
    37441 atcaccaaat aaaggattta atcttggtgg attaaagact tcttattcaa tagttataaa
    37501 taaggatata agggacaaat ttagaaataa attaaagcaa aattctataa cttctccaaa
    37561 tgtgtttgga ataataggat tgataaccgc ttataatgaa tgtcatgagt ggttaagtgg
    37621 tgtaaatgaa tatataaaat caaattatga acttttagaa acttgggtaa ataaatataa
    37681 taagataaaa ttgatgaaaa tggaatcatc ttacttagct tggatggata taagtggatt
    37741 aggtattagt gcaagtgagt ttacagataa attggctata gatacaggag tattattaga
    37801 agatgggagt cattttgtaa aagatggtga gaagtatgtt agaataaatt taggaactca
    37861 aaaggaaaat gtaattgaag ctttaaatag aatggattta tttttaaagt ctttataaaa
    37921 atttggttga attagtattt tatataaatt taaactatta atatggtata tacataagta
    37981 gaatttgttt tagattactt aagtatatac catattttat ataaaaatta aatttgcatt
    38041 agtcatgtga taactattta aattcatatg tattttgtat gtataataga tataattatt
    38101 atttaaactt attgtttatt taatttttca atagaaaaag atttatctgt aagtttaaaa
    38161 ttatctttta tacccttagt taagtatact ttcttatcct tagttatgaa tatagcatct
    38221 acattatcaa ttttattaat taaattcata cctttttcaa gaccaagacc aaatgtagaa
    38281 gtggataagg catcacaatt aatagattta tcagagatta ttgtaacact acttaaattg
    38341 ttttcaaaag gataccctgt aaatggattt aacatatgat gatatatttt tccgtttttt
    38401 tcgataaatc tttcataaat tccagaagta acaacagact tatttgtagt ttctatgttt
    38461 cctatggatt caccattttt actagttggg tcttgaatac ctattttaaa aggtttatca
    38521 tttttaccat tttctaaagt aaatatattt cctcctaagt ttactaaacc tttttttacg
    38581 tcctctgatt ttaaataggc tacaatttta tcagcagcat atcctttagc tatggcacct
    38641 aaatcaattt tcatattttc tttggttaat tttatagatt tatttttttc atccaatatg
    38701 atgttcttat aatcaataag aggcagaagt ttttgtatct cagaatcact tggaacttta
    38761 gctttatctg ttccaatagc ccataaattt gataatggac caattgttat gtcaaaatat
    38821 ccatctgata atttggaata ttgtatagct tcttttacga cgaaaaatgt atcatcagaa
    38881 actctgacaa attttttacc agcattatca tttattttag aaacatcact tcctggtata
    38941 tgagtgctca ttttgttgtc aatatatctt aaaattgaat cacattcatt taagatttta
    39001 tcacttttgg attcttttac attaaataca gtaacttcgt ttactgtacc aaggtagtag
    39061 ctagttttac tatatttagt attctggctt gaactcctaa aaaatgaaaa taaaattaat
    39121 gctaaaatca atatcacaaa aataaatgtg atttttttct tattcatggt atattcctcc
    39181 tatgaaattg tgttattatt ttattataac ataatttcat aggaggacaa aaaatgaaga
    39241 aaaaagacat tcttcttatt ttaggcattt tggttgttat tacagcatgt tatgggataa
    39301 taaatgttat aaattctaaa aatgctggaa atattgagat ttatgtagat aataaattgt
    39361 ataaaactgt ttcaataaat gccaaagaag aatttaaaat agagaataga ggggggtata
    39421 acatagtaaa gattcatgat aaaggggttg agatagtaga tgcttcatgt cctgataagg
    39481 tttgcgtaca tactggattt ataaacaagc caagtcaaag catagtttgt atacctaata
    39541 gagtgagcat aaaaataaat acaaatgaaa agaatgataa tcaagaagat ataatttcta
    39601 aataaaataa taaaaataat tgaaaggaat tggtgataat tatggttaac ttaggaaacg
    39661 attgggatga attattgaaa gaagaatttg aaaaagatta ctacttaaac ttaagaaaat
    39721 ttcttataga agaatataaa acacgccaaa ttttcccaaa tatgcacaat atatatgaag
    39781 ctttaaaaca cacttcatac aaagacacaa aagtcttaat attaggacaa gacccttatc
    39841 atggagataa tcaagctcat ggtttagctt tttcagttca accacaagtg aaaactccac
    39901 catctttatt aaatatgtat aaagaattaa aagatgattt aggttgtttt atacctaata
    39961 atggatattt aatgccttgg gcagaccaag gagtgttact tttaaatact gctcttactg
    40021 ttagagcgca tgaagctaat tctcataaga acaaaggatg ggaaattttc actgatagag
    40081 taatatctat tttaagtgag cgtgaagacc cagttatttt tgtactatgg ggaagtaatg
    40141 cacgtaaaaa agtagagttg attgatacaa gtaaacatta tattttggaa gcacctcatc
    40201 caagcccatt gtcagcaagt aaaggctttt ttggatgtaa acacttctct aagataaatg
    40261 aaattttgaa aaagctagga aaagaaccta ttaattggca gatagaaaat atataaaagt
    40321 aaaaggtaac aaaatgataa caaaacaaca ataaacaatg acattgttgt aactaataga
    40381 aatatgctta aatagttgaa aatactatgt atgcatattt ctattttttt gtcaagttga
    40441 caactcttga aaaaatagtt acaatttagt tacacaaaaa acacaaataa aaaaagaaac
    40501 ttacaaagca gaattttatg aagcaaaata tatagtgtaa aaaatcttag ggaggaaatt
    40561 ttatatgaat tttaaaaaat tgagtaatgc taaagtctcg gtaatggcag ttgttttatc
    40621 agttggaata ttatcgggat actctgcgct taataaacaa gtaacattag tagtaagggg
    40681 agaagaaaga actatatcaa catttagttc taatgtagga gaccttttag aatctcaaaa
    40741 cattagctat gatgcaaatg atatagtaac tttagatgaa aatacaaagt tatctaatgg
    40801 agataaaata gaagtaattg atgtaaagga aaaaacagtt gtagaaacta aagacattcc
    40861 atttgaagtt acagttgttg aagatggctc tttattaaag ggtgattcta aggttaaaga
    40921 agaaggaaag tctggtaaaa gtgaattaat atataaagaa acttatcaca atggaaagaa
    40981 agtagaaaag gaatttgtga aaaaggttgt taaagctgag ccagtaaata aggttgtaag
    41041 caaagggaca aaggtagaaa tgcaagtagc ctcatcaagg ggagaaagtt caagaagaat
    41101 ggcttcatca tcatctagta gcagtagtag taacaatggt aaaaatatga gagtggtagc
    41161 aactgcatat actggtcaca gtataactgc aacaggaaca aaacctaagt ggggaacaat
    41221 agctgtagac cctaaggtta taccatatgg aactaaagtt tatatacctc aatttgacat
    41281 ggtgtttact gcagaagact gtggtggagc aataaaagga aataaaattg atatctacat
    41341 gaatgattca aaaacagttt ataactgggg aagaaaaact atagatatac gtatattaaa
    41401 atagttaaat aatttatatt tttactggaa attatatttt gattatatag agaagaaact
    41461 ctttatagat tctaaaaata taatttccag ttttttattt tggatatgta ataaaacagg
    41521 taaattttaa ccatgtatag ataaaagcta tttaaattat aatttactat catataaatc
    41581 tataaaaatt gttgcaaaga gtcaaaatat atgatattat agatttcgtt gagaaggatt
    41641 tcattatggg agtggatggg tgccggtgtg ccctctagtc ttcaaaacta gtatgagggg
    41701 ttaagagcct cttaggtggg ttcgattccc acgcattccc gccaatatgt aaaaggtatc
    41761 ttttaattaa aagatacctt tttaaatttg ttttaagata atttagtttt aatgatatat
    41821 aatattttgt attatataat gtaaatttta taatactatt attgttcaga taaaagtcta
    41881 ggagagtgaa aagatggaaa ataattttac aacgagaact agttttctag ttggagatga
    41941 tgggatagaa aaacttaata attcaaatat aatagtattt ggagttggag gagttggtag
    42001 ttttactgtt gaagctttag caagagctgg agtaggcaat attactattg tagattttga
    42061 tgatgtagat ataaccaata taaatagaca aataccagcc ctacattcaa ctgtaggaag
    42121 atacaaagtt gatgtcatgg aagaaagaat attagatata aatcctaata tcaatataaa
    42181 aaaaataaga agtctttata ataaagatac tagtgacgaa atactaactg aaagatatga
    42241 ttatgttgtg gatgcgatag acatggtaag ttccaaaata catctaattg aaacttgtga
    42301 aaaaaaagga ttgaaaataa taagttctat gggtatggga aataaattag acccaactaa
    42361 aatagtcgtt acagatatac acaaaacaag tacttgtcct ctagctaaag ttatgaggaa
    42421 agaacttagg gatagaggta tcaaaaagtt aaaagtagta tattctactg aacaaccaat
    42481 agaacttaag aagaaagtaa tgaatggtag aaaagtaaca cctggtagtg tatcatttgt
    42541 cccatctgta ggaggtttaa taatcgcatc tgtaatagta aatgaactat taggacaata
    42601 agttttaaat attttaattt ataaatttta tatacaaatt tgaaggctag aaggatgaat
    42661 ataaagcaat tttatattca tacttttagc ctagttattt tatattacct tattttatgt
    42721 agttgtagct ttatttttta gattatgtat ctatttcata attatatttt gtaaatataa
    42781 tttctccatc actataaaaa acaatatctc ctattctgtc tgttctataa attgatacat
    42841 ggttttgttt tagattatta ataacctccc tatgtggatg accatattga tttttatatc
    42901 cacatgatat tgcagctata tctggactaa ttttttttat aaattctaaa gatgaagaag
    42961 aattacttcc atgatgacct atttttataa aattaatatt atttaattta aaagaatgta
    43021 aaatttcttt ttcattttct tcttcagcat cacccataaa taaaaaagaa ttatcattaa
    43081 aagtta
//
LOCUS       DAYAOY010000026        42614 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYAOY010000026 DAYAOY010000000
VERSION     DAYAOY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.026"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(125..790)
                     /locus_tag="ACGI6B_001491"
     CDS             complement(125..790)
                     /locus_tag="ACGI6B_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750939.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            1134..2564
                     /locus_tag="ACGI6B_001492"
     CDS             1134..2564
                     /locus_tag="ACGI6B_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9750940.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2932..3510
                     /locus_tag="ACGI6B_001493"
     CDS             2932..3510
                     /locus_tag="ACGI6B_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFG9750941.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            3564..3686
                     /locus_tag="ACGI6B_001494"
     CDS             3564..3686
                     /locus_tag="ACGI6B_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFG9750942.1"
                     /translation="MKFASSLALSTAILSANSTCPWIIHQPKVPKEISNLKKTN"
     gene            3982..5361
                     /gene="scfB"
                     /locus_tag="ACGI6B_001495"
     CDS             3982..5361
                     /gene="scfB"
                     /locus_tag="ACGI6B_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HFG9750943.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            5390..5887
                     /locus_tag="ACGI6B_001496"
     CDS             5390..5887
                     /locus_tag="ACGI6B_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFG9750944.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            6185..7849
                     /locus_tag="ACGI6B_001497"
     CDS             6185..7849
                     /locus_tag="ACGI6B_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HFG9750945.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKILNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            7862..10312
                     /gene="recJ"
                     /locus_tag="ACGI6B_001498"
     CDS             7862..10312
                     /gene="recJ"
                     /locus_tag="ACGI6B_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFG9750946.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            10325..10837
                     /locus_tag="ACGI6B_001499"
     CDS             10325..10837
                     /locus_tag="ACGI6B_001499"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFG9750947.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            10911..13118
                     /locus_tag="ACGI6B_001500"
     CDS             10911..13118
                     /locus_tag="ACGI6B_001500"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFG9750948.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            13143..13592
                     /gene="dtd"
                     /locus_tag="ACGI6B_001501"
     CDS             13143..13592
                     /gene="dtd"
                     /locus_tag="ACGI6B_001501"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFG9750949.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            13607..14221
                     /locus_tag="ACGI6B_001502"
     CDS             13607..14221
                     /locus_tag="ACGI6B_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9750950.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            14312..15868
                     /locus_tag="ACGI6B_001503"
     CDS             14312..15868
                     /locus_tag="ACGI6B_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HFG9750951.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            15858..17120
                     /gene="hisS"
                     /locus_tag="ACGI6B_001504"
     CDS             15858..17120
                     /gene="hisS"
                     /locus_tag="ACGI6B_001504"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFG9750952.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            17194..18981
                     /gene="aspS"
                     /locus_tag="ACGI6B_001505"
     CDS             17194..18981
                     /gene="aspS"
                     /locus_tag="ACGI6B_001505"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFG9750953.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            19440..20672
                     /locus_tag="ACGI6B_001506"
     CDS             19440..20672
                     /locus_tag="ACGI6B_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFG9750954.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            20686..21492
                     /locus_tag="ACGI6B_001507"
     CDS             20686..21492
                     /locus_tag="ACGI6B_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HFG9750955.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(21604..22473)
                     /locus_tag="ACGI6B_001508"
     CDS             complement(21604..22473)
                     /locus_tag="ACGI6B_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9750956.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            22845..24272
                     /locus_tag="ACGI6B_001509"
     CDS             22845..24272
                     /locus_tag="ACGI6B_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HFG9750957.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(24349..24618)
                     /locus_tag="ACGI6B_001510"
     CDS             complement(24349..24618)
                     /locus_tag="ACGI6B_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750958.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(24621..26063)
                     /gene="nhaC"
                     /locus_tag="ACGI6B_001511"
     CDS             complement(24621..26063)
                     /gene="nhaC"
                     /locus_tag="ACGI6B_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFG9750959.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(26088..27254)
                     /locus_tag="ACGI6B_001512"
     CDS             complement(26088..27254)
                     /locus_tag="ACGI6B_001512"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9750960.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(27479..29599)
                     /locus_tag="ACGI6B_001513"
     CDS             complement(27479..29599)
                     /locus_tag="ACGI6B_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9750961.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            29832..30233
                     /locus_tag="ACGI6B_001514"
     CDS             29832..30233
                     /locus_tag="ACGI6B_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750962.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            30276..30569
                     /locus_tag="ACGI6B_001515"
     CDS             30276..30569
                     /locus_tag="ACGI6B_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFG9750963.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            30584..31024
                     /locus_tag="ACGI6B_001516"
     CDS             30584..31024
                     /locus_tag="ACGI6B_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HFG9750964.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(31116..31589)
                     /locus_tag="ACGI6B_001517"
     CDS             complement(31116..31589)
                     /locus_tag="ACGI6B_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9750965.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(31589..32377)
                     /locus_tag="ACGI6B_001518"
     CDS             complement(31589..32377)
                     /locus_tag="ACGI6B_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9750966.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            32705..33949
                     /gene="glyA"
                     /locus_tag="ACGI6B_001519"
     CDS             32705..33949
                     /gene="glyA"
                     /locus_tag="ACGI6B_001519"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFG9750967.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            34272..35369
                     /locus_tag="ACGI6B_001520"
     CDS             34272..35369
                     /locus_tag="ACGI6B_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9750968.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            35360..36058
                     /locus_tag="ACGI6B_001521"
     CDS             35360..36058
                     /locus_tag="ACGI6B_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9750969.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            36061..37125
                     /locus_tag="ACGI6B_001522"
     CDS             36061..37125
                     /locus_tag="ACGI6B_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFG9750970.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            37308..38012
                     /locus_tag="ACGI6B_001523"
     CDS             37308..38012
                     /locus_tag="ACGI6B_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9750971.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            38005..39327
                     /locus_tag="ACGI6B_001524"
     CDS             38005..39327
                     /locus_tag="ACGI6B_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9750972.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            39514..40887
                     /locus_tag="ACGI6B_001525"
     CDS             39514..40887
                     /locus_tag="ACGI6B_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750973.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(41259..42197)
                     /gene="pdaA"
                     /locus_tag="ACGI6B_001526"
     CDS             complement(41259..42197)
                     /gene="pdaA"
                     /locus_tag="ACGI6B_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFG9750974.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        16399 a         4863 c         7193 g        14159 t
ORIGIN      
        1 ttttatatag aattaaatat attttattat ttcaacaatg aaaaagaagc ttatctctat
       61 tttgagacag cctctttgta tactattatt aatttaatca ataacataat ttaaaactat
      121 attattagtt aagatttcct gctttattag taaataattc tttaatagca ttgtttctgg
      181 aaactaaatc gttatcagag ttgtttgttt ttttcataat cactgaataa cgttttttat
      241 ttataaaaaa tattaaaatc ttatcatcat caaaattgta ttctttatat ctgtcaatag
      301 actcataacc tagatttaga atgtttttgt cttttatact atctacatct aaaatatatg
      361 atgggtcagc ctttagtaat atattaatta aacatttttc ttcaccagca cagaataact
      421 ccatcttact tgatatagat ttagattgct ctgaagttat ttttgcagga ataaggttgt
      481 cattgtcatc tcttagaaga taaccatgtg tgtcttgttg atatactaaa ttaacctttt
      541 tcttaggaac actaatcatt ttatctaaat gtacataatg atgattttca acaaaacctt
      601 catttaatat ttcaacaaag ttattaattt tacctaggca aaaagattct atagattctt
      661 tacagaattt atttgtaaat ggtattatgt ttacgttttc ctcgtctgta ttccatgcta
      721 tagcataatc caacgaatat acttcttcct ctatatctgc aaaattatat tgtactatgt
      781 ttcctttcaa aattactcga cctccatctt ataatatatt taatatactt tataaaactt
      841 atattatatt taaataatat cataaattga ataaatatga tatagtaaat aagaaaaaat
      901 ttgaaaaaaa tcactataaa tgagtaataa atataaatag tgtatactaa tgggatggaa
      961 aatgtccact attaacttta ttaccccaca tatataagat aaaattatta atataaaata
     1021 taatctatcg acaatgaata gacttttaaa taaacttaat ataaaattaa ttaaataagt
     1081 gcttaaaaac gatttacttt aatgatagaa tcatattaaa ggaggataat tttatggata
     1141 taaagaataa aattaaatct atatttttac cagaaaaaaa tgactattta gatgatgaag
     1201 aatttgagta tgatatagaa aataatgaag aagatttaaa ggaagataac aaagtacatt
     1261 taaatgattt aagcagagta aaatatgatt acagagagtt aaaagacata gaaaaccaaa
     1321 cagaagagat gtgtctgcta gcagtaaagc aggatggtac tataattgag tttgtaaagg
     1381 acaagactta taaagtatgt atggaagcag taaaacagac atataaatct cttaagtata
     1441 taacaaatca aaatgaggat atttgtatag aagcagtaaa acagaactac agagctttgt
     1501 actacataaa taataagaca gaaaatgtac taattgaggc tataaaaaat gcatcaactt
     1561 atgatgtcat ggaagtattt aaatttgtgg aagagcaaac agaagatgta tgtctagcat
     1621 ttatagaaag ggcatctaaa aatgatgttg cagagatatt aaaaggaata aaagagcaaa
     1681 ctccagccat atgtttagaa gctgtaaaaa aagatggaaa atctttagct tatgtaaagg
     1741 aacaaagtaa tagtatatgt ttagaagctg taaaagaaaa ctacagtgca ctatcttgtg
     1801 taaaagagca aactgaagaa atatgtatag aagcagtaaa acagaatgac tttgcattgt
     1861 attatgtaaa tgaacagaca gaaaaaatat gcatggaagc agtaaaaaga agttatatgg
     1921 ctctacagta tgtaaataag caaacagaag agatttgttt ggaagcagta agaattgatg
     1981 gaagagcatt acagtacgta aaagagcaaa cagaagaaat ttgcttggag tcagttagac
     2041 aaaatggaaa agtactgcaa tatgtaaaaa aacagactga aaatatatgt atagaagcag
     2101 taagaggttc atttgaagaa cttgaaataa aagaaatatt aagttatgta aagataccaa
     2161 cagaaagaat atttgtagaa gcagtaaagc aaaatggaaa aattcttaaa tatgtagaaa
     2221 atcagacaga attaatatgt ttagaggcag taagagaaaa ctataatgct ttagcttatg
     2281 taaaggaaca aacagaaaag atttgtttag aagcagttaa tcaaagttat gaagcgctta
     2341 aatatgtgaa agaacaaaca gaagaagttt gtctaaaagc agtaaaacaa gattatagaa
     2401 tgttgaaata tgtaaataat caaacagaaa aaatatgttt agaagcagta aagcaaaatt
     2461 atagagcttt agaatttgtt gataatcaaa cagaaaaagt ttgtcttgaa gcagtaaaac
     2521 agaatagaaa agcactacag tatgtaaaac aaaaacaaag ttaaaaggct aaaatatatg
     2581 gggaatatat gcaaataaat aaaatttgat attttgtaat aaataaaatg ggtatctcaa
     2641 aaggaacttt ttagtttgtg aggtactctt tttatatgaa atgaaatata tttttagcct
     2701 tttaacaaag gggaaaaggt ttgtttctat tttgacatag gctttttatg catataaagc
     2761 ttgataaaat tgttttatta taattgtttt gttatataaa taataagaag aaaattatat
     2821 aggtcttttt ttgaccagtt ttacattttt aacaactagt tttgtaaagt atattgaatt
     2881 ttctcaaaaa agaatgtact ctatatatgt aaaatttttt tggaggtaaa aatgtttaaa
     2941 agatatgctg aaaaaatgac atctgtttta atttgtaata atatgataga taataatgaa
     3001 tctaaagtct attcttatgg atttgaaata ttaatagctt tcatagttaa tataaccacc
     3061 atgctgttta taggtttttt atttggtaaa tttacatatg tattattttt tttaatgtgt
     3121 tattgtccaa taagacaatt ttcaggaggg tatcatgcag acaattattt tagatgctta
     3181 ttaactttta tttttataat tttatccaca atcttaatta tagaaaatat aaatatagac
     3241 ttatttaaaa atattattat gattattgca agtgttagtt gggttggaat ttgtgttctg
     3301 tgcccaatag aacataggag taaccctata agtgataggg aaaagttggt ttataagaaa
     3361 actgctatat ttatatctac agtagtgtta ttgataacaa tttcaagtct ttctttgagt
     3421 atatttgtag attatttcac atatagcgca tttgccatgt tttggatttt tgtgatgtta
     3481 gtattaggta agttaaaagc taaagtataa gtaaaatatg tatgagggag agttaagtta
     3541 tgaaaaagtt tattgtaaga tttatgaagt ttgctagctc attggcactt agcactgcta
     3601 ttttgtcagc taatagtacc tgtccttgga ttattcatca gcctaaagta cccaaagaaa
     3661 tcagcaatct aaagaagaca aattaaatac aagcacttat ttatacaaaa ttatcaggaa
     3721 aaaagaaatt attatatttt aataagatta aaacaatata aaatcagata caaaatttat
     3781 tttataggag cctaatacct atttggtaat atattagtgc gccaatttag ggggatatag
     3841 tgtatttaat gaaatgactt acggagagta agtcattttt gtattgtatg tattttatgt
     3901 aaacttatga tataatgaag ggtaaagttt aattaaaata ataaaaaact ttagtataga
     3961 tagtaattta ggaggtaggg tatgagtatg atacataaat tttctatgaa tggatataat
     4021 atcgttttag atgtgaatgg tggagctgtt catgtgttag atgatgttgc atacgatttg
     4081 ttagattttt ataaagaaaa aagtaaagaa gaaatattag agatattaaa atcaaaatat
     4141 caagaagaaa aaataaatga agcatatgaa gaaatattaa atttggaaaa agagggtctt
     4201 ttatatacag aagatacata tcaatatcat ccaagctttg tacatagaga gcctgtagta
     4261 aaagctttat gtttaaatgt agctcatgat tgtaacttaa aatgtaaata ctgttttgca
     4321 gctcaaggtg attttggagg agaaaaagaa cttatgagtt ttgaagttgg taaggcagca
     4381 attgactatt tgattgctaa ttcaggaagt agaaagaacc tagagataga tttctttggt
     4441 ggagagcctt tgatgaactt tgaagttgta aaacaactgg ttgactatgg aagaagtgta
     4501 gaaaaagatt ataataaaaa tataagattt acaataacta caaatggtgt actgctaaat
     4561 gatgagatta tagattatat aaatgaaaat atgcataatg tagttttaag tttagatgga
     4621 agaaaagaag ttaatgataa tatgagacca actttaaatg ataaaggaag ttatgatatt
     4681 acattaccac gatttaagaa acttgtggaa aaaagagcaa aagataagta ttattatata
     4741 agaggtactt ttacaagaga taatttagat ttttcaaaag atgttatgca ttttgcagat
     4801 ttaggattta aattaacctc tgtagagcca gtcgttggag atgagagtaa tccatatgca
     4861 cttagagaag aagatttacc taagatattt gaagagtacg aaaaatttgc tgtagagtat
     4921 gcagatagac aattacaagg agatggattt aaatttttcc attttatgat agacctaaat
     4981 caaggtccat gtgtaataaa gagaataact ggatgtggag ctggaaatga gtatctttct
     5041 gtaactccaa atggagatat ctatccatgt catcaatttg ttggaaatga agaatttaaa
     5101 atggcaaata tatttgatga agaaatagta cttccagaaa atttaaaaaa tatgtttaga
     5161 gaagctcatg tatatactaa agaagagtgt aaacaatgtt ggaataagtt ttactgttca
     5221 ggtggatgtc atgcaaatgc gataaacttt aataatgaca tatcaaaacc atatgaatta
     5281 ggatgtgaaa tgcaaagaaa acgtacagag tgctctataa tgatacaagc taagttaatg
     5341 ttagaaggag caaccaacta aatttatatt ttaaaggagt tgtttatata tgattagaga
     5401 ttatttagaa gataaacctt taattgacga aagtgtattt gtggcaaaat cagcagatgt
     5461 aattggaaat gttaaaatag gcaaagattc aagtatatgg tacaatgcag tagttagggg
     5521 tgatgaagga ccaataacta ttggagaaaa cactaatata caagattgtt ctatagtgca
     5581 tggagataca gaaactataa ttggtaacaa tgtaacagtt gggcataggt ctatagttca
     5641 tggttgtaaa attagtgata atgtgttaat tggtatgggt tcaataatat tggataatgc
     5701 tgaaatagga gaatatactc tgataggagc aggtacacta attacatcta ataagaagtt
     5761 tccgccaggt gttttgataa tgggttctcc tggtaaggtt gttagagagc ttacagaaga
     5821 agataaaaaa tatattgatg aatcttatga atggtattta gaggcagcac aaaatcaaaa
     5881 atattagttt aaagagtggt tgtttcaaaa catatttaaa tctgttttga ggcaaccact
     5941 aatttatttt attagatttt gttgatattt tagttttcaa aaagtatttg attaaaccag
     6001 atataaaatg tcaaaaataa aaattaactg agttaaataa ttttaataaa attggtagta
     6061 tatgtgataa aattaaatat taaagaggat ataggatatt agaatatcaa ttggtgaaat
     6121 aatgtcatat acgataaatt aaggtgtaag tttttgggta tacctatctt aggagtgaaa
     6181 agatttgaga ataagttata gaaatttaaa gcgatataga gaaattggat atgttttaat
     6241 aaagtatggt tttagtttta ttgtagaaag actaaatata gaaggaattg cctataaaat
     6301 accactattt gacccaccag aagaaataaa aaatatgaca actggtgaga gaatgaaaag
     6361 agttttagaa gaactaggac ctacatatat aaaaatagga cagatattga gtacaagaaa
     6421 agacttgtta gaccaggata taattgatga aatatcaaaa ctcagagatg atgtagagaa
     6481 atttgattca aatatagcta ttgatatatt taaagaagaa gtgggtttaa gtatagaaga
     6541 aatattctta gaatttaaag aagaagcaat agcagcagct tcaattggtc aagtttatga
     6601 aggtgtactt aaaacgggag aagaagtaat cgtaaaaatt caaagaccaa atatagagaa
     6661 aataataaaa tctgatttag agattcttag aactattgca aatactttaa aagatttaaa
     6721 aaaggatttt aatttagatt tagtacagat gattgaagaa tttcaaactc aattaatgag
     6781 ggaacttgat tatacatttg aagctataaa tgcaactaaa ttctctagga tatttaaaaa
     6841 tagtgatgaa gtatatatac ctaaagtata ttcagaatat aatactaaga aaattttagt
     6901 aatggagaaa gttaatggta caaagttaag tgatgtagaa aaaataagga gactaggtta
     6961 taacacaaaa actattgttg aaataggcgt taggtcattt tttacacagg tattatctca
     7021 tggatttttt catgcagacc cacatccagg taatatattc gtagttgcta aaaataagat
     7081 agcatatata gattttggaa tgattggaat tattgataat aaaattttaa accaattaaa
     7141 tgaaattgca ttagcaggtg tagaaaagaa tgttgacaag ataatatatc tattgataga
     7201 aatggatgca ttaaatggag aggctgatat taagggactt agacaggatt tactttatct
     7261 aatacattat tactatgata tttctataga aaaaataaat gtaacagata tattaaatga
     7321 actatttagg ttttttagac agtataaaat agtcatgcct gcacaatttg taactcttgc
     7381 aaagacagtt ataacattag aaggaacaag tagaacttta aacacagatt tttcttttgg
     7441 ttctatggga aaagagttta tgaaacatca ttataagagt aaatttaacc ctaaaaatgt
     7501 tgttttaagt tcaagacaaa atgtagaaga aatattactg gacataaaaa ctattccaaa
     7561 gcaattaaaa gcaattttaa aaaatataga aagaaataat ataaaaatgc aaattgaaga
     7621 tgttaaaatg acgaggttag aaaattgtat aatagagcta acatctcaaa tatctttgag
     7681 tttagtgttg gcatctatta tagtaggttc ttctcttata attgcatctc caaatataga
     7741 aaataatata tggataaaat ttactgcaat agcgggtttc tttatatcct ttataatagg
     7801 cctttgctta gttataagaa gtattaggtc aaaatacaaa aaagattagg gggaattaaa
     7861 tttgaaaaaa tggatattga agtataaagg taagatagag gaaagtgaat ttagcaaaaa
     7921 acttaatatt tccccggaaa tctgccaaat tttaaaaaat agagggatat gtactgaaaa
     7981 agattcagag atatttatga atccatcttt agattattta agagaccctt ttttaatgaa
     8041 agatatgcaa aaagctgttg atagaataaa gttagcagtt gagaagaacg aaaaaatttg
     8101 gatttacgga gattatgatg ttgatggtgt atcatctaca tctatcttgt gcttatattt
     8161 taagagtata ggttacaatg ttgattacta tattcctaat agattagaag aagggtatgg
     8221 tataaatgaa gatgctatta agcttataag ctctagggga tgtgacttga ttataagtgt
     8281 tgactgtgga attacatcag tgtctgaggt caatacagcg aatgatttgg gcatagatgt
     8341 tataattaca gaccatcatg aatgtcaaag tgaaattcca tcagcctttg cagtaataaa
     8401 tccaaaacaa gaagattgta attatccatt tgattcacta tgtggatgtg gtgtagcatt
     8461 taaaatgatt caagccttga ctccaaaaga agaatttaaa actagtatgt ataattattt
     8521 ggaaatagta acactagcaa ctatttgtga tattgttcca cttatagatg aaaatagaat
     8581 aattgtaaaa aatggtttaa aatcaatgaa agaaggcaaa aatattggat taagggagct
     8641 tataaaagtt tgtggtgtag aaagtgataa gataggttct tctcatatag gttttgcaat
     8701 aggtcctagg ataaatgctt caggtcgatt gggatattct tacttaggag tagagctatt
     8761 tactactcaa tcacaagaag aggcagtaga aatagctagt atattggaag aaaaaaataa
     8821 tgaaagacag atgatagaag ctaagatgta tcatgaagct gaggaaatgc taaagtctaa
     8881 tagcagatac aatgatgaca aggtgttggt tttagctaaa gagggttggc aacatggaat
     8941 aataggaatt gtagcatcaa agttaactga aaaatactac aaaccaacaa ttcttctagg
     9001 tatagaaaat ggggaggcta ctggttcagc gaggtcaata aaaggattta atatatttga
     9061 agctctcata aagtgcaaag acttgatgac taagtttggt ggtcatgaac aagcagcagg
     9121 gttatccttg gattcagaca atgtagaaat tttagcaaat gaaataaata aatttgcaga
     9181 ttataattta actgaagatg atatgataga gaatgtaaat gttgagtttg aattacaaga
     9241 aaatgtaata aatttaaatc ttgtagaaga attacataag ttagaaccct ttggattaaa
     9301 taatcctaat cctagattta tagttagaaa ttatatttta aaagatttga aagttatagg
     9361 taagaatcaa caacatctga aattaagcat agaaaaagag aaaagctatg aatgtatagg
     9421 atttaatatg agtcatttaa aatcaatgta taaagtagga gacaaggtag atgtactatt
     9481 ccaattagat gaaaataact atatgggaaa taggaaagta caatttttac ttaaagatat
     9541 acgattggct cgacctaaaa gtgcaagtaa tgacaaactt tcattgaaat taatgtcaaa
     9601 aataatacca aaagatactc aaagcctgta taatatatct gtgtctgatt ttgaattatt
     9661 tgatggaaat acagatataa atatatttga ttattttgaa aaagatacac taataatatc
     9721 taattctata aatggatttt atagagcaat gtcagatata tcacttatag atttggattt
     9781 taacataaat tacaatatta ttgaagatgt tagcaaaaat actgataaat tagagcttat
     9841 attttctcct aatattgata aaatagactt aaaaagatat aataatatta ttctttatga
     9901 ttatttatac aataaaggcg aatattcgta tatatatgag aataaaagag aagaaagtga
     9961 aattataaaa tattataata aaacagacct attatattta aagaatgtag ttagtaatat
    10021 agttccatca agagatgaat ttataacaat ttataaacaa gcactcataa aaaaagaaat
    10081 tgatttggat atggtaaata taagggaaac ttttaatgta attccattaa agttttttac
    10141 gatattaaat gtatttagag aattaaattt acttgacttt aatttaaatt atgaaaaaaa
    10201 ttctgtactc ataagaatat tgccaaaacc acagaaaaaa ttagacttaa atgagagttt
    10261 aatactcaat aatctaaaaa atttagaaaa acaatacaat agcagttatt aggaggcgtt
    10321 tcccatggat ttaaaaaact ttataaggaa tattgatgat ttcccaaagc caggcataga
    10381 ttttaaagat gtgacaactc tatttaaaga tggagatgca tttaaatatg cagtggattc
    10441 tatagttgaa gaattgaaag ataaagatgt agatttagta ataggaccag aagcaagagg
    10501 atttttgatg ggtacacctg tagcatatgc tttaggagta ggatttgttc caattagaaa
    10561 acctggaaag ttaccaggtg aagttgagag ttatgagtat ggattagagt atggtacaga
    10621 tactttagag atacataagg atgcaattaa aaagggtcaa aaagtagcaa tagtagatga
    10681 tttattggct actggtggaa ctatggaagc cgctgccaag cttgtagaaa aattaggtgg
    10741 agaagtagtt tctatgcaat ttttgattga gctaaaattc ttaaatggga gagaaaagtt
    10801 gtctaactat gatgttaatt ctcttataaa atactagtac ttttataata gttggtataa
    10861 caccaactat tttcatatat attaaaattt tatacaaaat aggtggttat atgcatgata
    10921 aagaattaca agaaataatt gaaaaaataa aagtttatgc acctaatgca aattttgaaa
    10981 tggttgaaaa agcctataat cttgcaaaaa aagcgcatga aggacaatat agaaaatctg
    11041 gggaaccata ctttattcat ccattagcag ttgcaaatat tttaatagag atggaattag
    11101 atattgaaac tataacagca ggtttacttc atgatgttgt agaagataca gaatatacct
    11161 atgaagacat aaaaaaagaa tttggcaaag aagtagcgga tttggttgat ggagtaacta
    11221 aacttggtca aataaaatat cgttcgaaag aagaaactca atctgaaaat ttaagaaaaa
    11281 tgtttttggc aatggcgaag gatataaggg ttatacttat aaaacttgct gatagattgc
    11341 acaacatgag aacattaaaa tacatgcctc cagaaaaagc aaagtataaa gcaaaagaga
    11401 cacttgaaat ttatggtggt atagctcata gattaggaat ttccaagata aagtgggaac
    11461 tagaagacag agcattaaga tttatggacc cagaagggta ttatgattta gttagtagag
    11521 tatcaatgaa gagaagtcaa agagaggatt atattcaagg aattgttgaa cttcttaaag
    11581 ataagtttaa agatttaagt ataccatgtg atgtatatgg tagaccaaag catttttaca
    11641 gtatatatag aaagatgcaa aaaaaacata agacttttga agagattttt gatttaacag
    11701 cagtaaggat attggttgat aatgtaaagg attgctatgc agtacttggt atagttcata
    11761 cattatggag acctattcca ggaagattta aagattatat agcaatgcca aaacccaata
    11821 tgtatcaatc attacacacc actgttgtag gtcctgatgg agaaccttta gagatacaaa
    11881 taagaactca tgagatgcat aatatagctg aaaatggtat agcagcccat tggaaatata
    11941 aagaaggtat ctctagcaaa gaatcaaagg ttgaagaaaa acttcaatgg ttgagacaga
    12001 tgatggagtg ggaaaaagat ttaaaagacc cacaagagtt tatggatgca cttaaagaag
    12061 atgtatttaa tagtcaagtt tatgtattta cgccaaaagg agacgtaatt gaattaccag
    12121 cagaatcaac acctatagat tttgcctata gggttcatac aaatgtaggc aataaatgtg
    12181 taggagctaa aattgatgga agaatagttc ctattgatta taagcttcaa aatggtaata
    12241 ttgtcgaggt acttacatca tctaattcta atggaccaag tagggattgg cttaatatag
    12301 tgaagacccc aaatgcaaaa agtagaatta gacaattttt caagaaggaa agaagagaag
    12361 aaaatgttga ccgtggaaat acaatattag aaagagaatt taagaagcat gatataccac
    12421 aaaaagaccc tcttgttgaa aaatatatgg cattagttgc taagaaattt aatcagccaa
    12481 gtgttgagga cttaattgcc actgtaggtt atggtggtat aatgtcatct caagtggtat
    12541 ctaaggtaaa agacctatat ttaaaagagg ttaaaaaagc taaaaaggaa caacagatac
    12601 aagaagaaga tttaaataaa cacaatataa gtgaaacaga atacagtaaa aagagaaaga
    12661 aaaacgcatc acaaggaatt atagtaaaag ggcttgacaa tatcttagta agatttgcta
    12721 agtgttgtaa tcctcttcca ggagatgaaa ttgtaggata tattactaag ggtaggggag
    12781 tagccataca cagagctgat tgcccaaatg caaacaataa tggtgatttc ttccaaaata
    12841 gattggtgga ggtttcatgg agttcagcag gaaaaggtaa gtttgaagcg gaaattcaaa
    12901 taaaagctat tgatagaaga ggaattgtta atgaaataac tcatattgtt gcaatggata
    12961 aacttagttt aaacggaata aatgcaagaa aaggtaagga tagtattgta agtataaact
    13021 tacttgtaga ggttaatgac acagaggaat taaaaattct aatgaaaaaa ttaaaagcaa
    13081 ttcctggtgt agaaagtatt tatagaatga caaattagag ttaaaataat agggaggaaa
    13141 ctatgagagc agtagtgcaa agagtatctt catctaaagt tacagtagat gaaaacacta
    13201 taggtcaaat aaataagggg ttactagtat tgttaggagt gactcatgat gataaatcaa
    13261 gtgatgtaga ttatatgatt gataaaatat tgaatctgag aatttttgaa gatgaaaatg
    13321 acaagatgaa tttatctcta atggatatag gtggagagct tttagtagta tctcaattta
    13381 ctttatatgg agattgcaga aaaggaagaa gacctggatt tagtaatgca gctaggccag
    13441 aacttgcaaa taacctttat gaagaatttg taaaaaaagc aaaagataaa ggtgtgacag
    13501 ttggtacagg tcaatttgca gctcatatga tggtagagtt aactaatgat ggtccagtaa
    13561 caatactatt agactcaagt aaatcttttt aagggggaaa actttaatga ttataaaaaa
    13621 atttgtagat agttattttg gagtaaatac ttatgtatta ggtgatgaaa agactaaaaa
    13681 atgtgcagtt atagacccag gtggaagttt agtagagaca ttaagttttg taaaagaaaa
    13741 tgaattaaat attgaatata ttatccttac tcatgggcat ggagaccata taggatatgt
    13801 taaggatatt aaagaaaaaa ctggtgctaa agtagttgca catgttgatg aaaaagaatt
    13861 attaaatgac aaaaataaaa atctaagtta tacaatgaga tgtggagcgc aagagtttga
    13921 tgcagatatc tatgtaaatg ataaagacaa gcttgaactt ggtgaattaa aaatgacttt
    13981 cttgcatact ccaggacata ctcaaggatg tatgtgtata agagttgaag atgaaatgtt
    14041 cacaggagat actttgtttg ctggaagtat tggtagaact gatttatata gtggagactt
    14101 taaccaaatg gaaaaatcac ttaagaaatt atgtaaatat gaagatgctg ttagagtata
    14161 tcctggacat ggaccaacca gtactctagg ggttgaaaag aagacaaatc cttacatgta
    14221 attttatatt agtatttaaa aaagtataaa atatttaata actatattag tgagggggtt
    14281 cttctactgt taaatagaag gagttaagtt gatgttgtat gtttttttaa aaggacatga
    14341 ttataagtat gaagtagcag aacttataaa attgtttact agtgaattta agtttaaaga
    14401 ctctaatgct aacaataata tagagtcaaa gcatttaata aatagattga tttatgagaa
    14461 tggtgtatta ttctcaagta ctgaatacta tgaaaatgga aacttaaagt acgagtcaat
    14521 acagaatata gaaaatatga atcttgaatt tttagatgga tttgatagca gcaaaacgat
    14581 tgaagaattt cttaatagcc tagatagtga aagtaagagg aactttaaaa agctgtgtaa
    14641 agaaaatatc aaaaaaagca tgtttttagt gcttaaacaa gtatttaatt cttatgtacc
    14701 atggggaatt ttgactggta ttagaccagt caaaattgtc cacaatttaa tggataaaaa
    14761 gctagatgat aatagcataa gaaccattct aaaagataat tattttataa tagatgagaa
    14821 aatagattta gctcttgaaa tagctaaaag agagcgatta tttatgtatc ctatagataa
    14881 aaataaaatt tctctttatg taagtattcc attttgtcca actagatgtg tatattgttc
    14941 atttccttca aattcattaa aacaatttgg acatttaaaa agagaatatg tttataaatt
    15001 gattgaagaa attaaggggt ttgccaaagt tattaaggat actaagaaag aagttgaaac
    15061 tctatatatt ggtggtggaa ctccaactac acttgatgag gaagaattgg atttgcttat
    15121 aaattcattg ttcaatgaat tagatttaag taaaattaaa gaatttactg tagaagcagg
    15181 tagacctgac actattacag aacaaaaact aagagtgtta aaaaaacaca atgttagtag
    15241 aattagtata aatcctcaaa ctatgaatga tgataccttg gtaaagattg gaagagagca
    15301 tagagtatct gatttagtag attgctttaa tatggctcgt agaatgggat ttgataatat
    15361 aaatatggat ataattttag gtcttataga tgaagactta aatatggtaa agaacacatt
    15421 ggaggagata aaaaaacttt ctcctgaaag ccttacagtt catacattag ccataaagag
    15481 agcatctaac ttaaatataa atatggatag gtataaagaa catctaacac aatatgaaga
    15541 aatggtaaaa atgatagatt tatcaatgaa atatgcaaaa gatatgggat taaatccata
    15601 ttatatgtac agacaaaaac atatgttggg aaatttagaa aatataggtt atgctaaaga
    15661 aggatatgag tgcatttata atatacagat tatggaagaa aaacaaagta actatgcatt
    15721 aggtgcaggt gctatatcga aatttgtcta tgtagatgaa gatagaattg aaagagtaga
    15781 aaatgtaaaa aatgttgaac aatacattga aagagtagat gaaatgataa aaaggaagaa
    15841 agaagaggta tataaaaatg ttgactaaag ctcctagagg aactaaagac ataactccaa
    15901 aagaggctta caaatggcgt tatgtagaaa ataaatttag agaaatatgt gctttatatg
    15961 gatatgaaga aatggtaaca cctatatttg aacatacaga acttttcaag agaagtgttg
    16021 gagatactac agatatagtt caaaaagaaa tgtattcttt taaagataaa ggtgatagag
    16081 aaattaccct aaagccagaa ggtacagcag gtgtagtaag agcatttata gaaaataaat
    16141 tatatgcaga tactcaacca acaaaattat tttatgtgac accttgtttt agatatgaaa
    16201 gaccacaagc tggtagacaa agacaatttc accaatttgg aatagaagca cttggaagtg
    16261 atactccttc aatggatgct gaaataatcg cactagctgt tcaatttttt aatgaagttg
    16321 gattaaatga tttggtagtg agtataaatt cagttggctg tccagtttgt agaaaagagt
    16381 acaatgcttt attaaaagag tatcttgact caaaagctga tatattgtgt gatacttgca
    16441 atgagagaag agaaaaaaat ccaatgaggg ttatagactg taaaaatcct acatgtaaag
    16501 aaaatatcaa agatatacca tttattgcag accatttgtg tgatgattgt aaatctcact
    16561 ttgataaact tcaagaatac ttaaaggaaa tgaatataaa ctttgtaatt gataaaacaa
    16621 tagttagagg cttagattac tatagaaaaa ctgcttttga aattatatct aatgatatag
    16681 gagctcaaag tactgtatgt ggtggaggaa gatatgatgg tcttgttgaa caattaggag
    16741 gaccaaaagg tataagtgga attgggtttg gactaggaat tgaaagactt ttacttacac
    16801 ttgaaggtaa tggaattgaa atagaaaacc ctcaatctac ggatatattt atagtaacta
    16861 taggtgaaga agcaaataca agaagcttta aattgttaaa agacttgaga caaaatcata
    16921 taagtgcaga taaagaccat atagagagaa gtgttaaagc acaatttaaa tattcagata
    16981 aaataaattc aaaatttaca atagtaattg gagatgatga acttaaaaat gacactgcga
    17041 cattaaaaaa tatgaaaaca tcagagcaaa ctactgtaaa attaagtaca ttagttgaag
    17101 aattaaagca aaagctgtaa aataagaaag tatgatgttt tgtgtattag ataaatatat
    17161 agcattaaaa ttaaataaaa tggaggtagt atcttggaaa ctttaaaggg tttaaagaga
    17221 actcactact gtggagagtt gagagaaaaa aacataaatg aagaagtcgt acttatggga
    17281 tgggtacaga aaaaaagaaa tttaggtggt cttgtatttg ttgatttaag agatacaagt
    17341 ggattatgtc aaatagtttt tgatacagat gtagataaag aagcttttga aaaagctgaa
    17401 aaattagggg ctgaatttgt aatagctgta aaaggaaaag tatgcgaaag acaatctaaa
    17461 aaccctaata tgccaactgg tgatatagaa atatttgcaa ctgaacttag acttttaaac
    17521 aagtcagaaa ctccgcctat ttatataaaa gatgatgatg atgtttctga agcactaaga
    17581 ttaaaatata gatacttaga tttaagaaaa ccatctatgc aaagaaattt aaaattaaga
    17641 cataaagtaa tgaatattac aagaaattac ttatcaaaca atagattttg cgaaatagaa
    17701 acaccatttt taatagctcc aacaccagaa ggagcaagag attatctagt accaagtaga
    17761 gttaatccag gaaaatttta cgcattacca caatcaccac aattatataa acaattattg
    17821 atggtaagtg gtatggatag atatttccaa atagttaaat gctttagaga tgaagactta
    17881 agagcagata gacaaccaga gtttacacaa atagactgcg aaatgtcttt tgttgagcaa
    17941 gaagatgtta tgagtatgat tgaaggtttg cttgaagcga tatttaaaga agttttagat
    18001 gtagaattgg cacttccact tcctaagatg acttatgcag aagctatgtc taaatatggt
    18061 tcagataaac cagatactag atttgggtat gagttaacag atatatctga tgttgtttgt
    18121 aactgtggat ttaaagtgtt tgctgatgct acacaaccag gaaaaagtgt tagaggtata
    18181 aacgtaaaag gcaaagctga cgactttact agaaaacaaa tatcatcact agaagaacat
    18241 gctaagacat atagagcaaa aggtcttgct tggatgaaag taggacaaga aggagtaact
    18301 tctccaatag caaaattctt caatgaagaa gaaatgaacg ctatacttac aagaatgaat
    18361 gctgaagtag gagatttact tttatttgta gctgataaaa actcaatagt atttgatgct
    18421 ttaggacaag ttagacttga agtagcaaat aggcttaatc tattggataa aaatgtatat
    18481 aacttactat gggtaactga gttcccagtt tttgaagaag atgaagaaac aggcacattc
    18541 tcagcaatgc atcacccatt tacatcacct atggatgaag atttagacaa gttagaagaa
    18601 ggtgataagt catctttaag agcgaaagct tatgatattg tattgaatgg ttatgaaata
    18661 ggtggaggaa gtgttagaat atcaaattct gatgttcaat caagaatgtt taaagcactt
    18721 ggttttacag aggaaagagc taatgaaaag tttggttatt tattagaagc atttaaatat
    18781 ggtactcctc ctcatgctgg tctagctttt ggtcttgata gacttgttat gttacttgca
    18841 ggaactgata atataagaga agttattgca ttccctaaaa atcaaaatgc tgtttgccct
    18901 atgacaaatg ctcctacatt ggcggaagat gagcaattag aagaattaag tataaaagtt
    18961 gatataaaag ataatgaata atatgtagtt gcaagaaact aaaatgtaaa aagtctggat
    19021 ataaaatata tttatattca gacttttttt attagaatta ttttatattt tttctctata
    19081 tattttcata aattatactt atattcttaa ccaatataat taaaatacta tgtttaaaaa
    19141 ttttacataa acttggttgt tttagaaact acttctgtaa cgagtacacg attttaatta
    19201 aagaatatat aacaaatttg aaataagtct aatatatgac ttaatagatt aaattaaaat
    19261 aataaatttg gactatttat ctatttataa cttaaattat atcaaattga taacaaacta
    19321 gatattatac ttttttgtag aatagaatta caattatata aagaaaaatt ctattaatag
    19381 tgaaaaaaaa gtagaaaaaa gtagaaatta ttcttttata aagaaaggga gggataatat
    19441 tgcaagagag agaagaatta gttttaaatc cagttccaaa agaggaaaga gttggatggt
    19501 tagctccatt gtttaatatg cttggttcta acatagctat atctgaacta atggttgggg
    19561 gaactttaat tttaggaatg actctttcta atatgattat tacatcaatt atagggaact
    19621 taatattagt tgcaattatt atgatacaag gttatatagg ttacaaagaa gggttaaata
    19681 cgtatgtatt agctaagggg gcatttggag agataggagg aaagtatctt atatctctat
    19741 tacttggtat aactagtttt ggatggtttg gtgtacaagc aggggttgca ggactatcag
    19801 tacaaaagat attcccaagt gtaaacctaa cattagttac agttatactt ggattattta
    19861 tggttgtttt tgcactgtat ggttttaaag ctatggctaa atttaattac ttagttatac
    19921 caccacttat gatattaatg gtgtggggag tttttaaagc tttttctact tatggagttg
    19981 aagctattta taattatact cctcaaacaa ctatgagtat ggttgaaggt cttaatattg
    20041 tagtaggtct tgttatagtt ggtgcaatta tatcacctga ccagttaaga tatacgagac
    20101 gagtaaaaga catttggatt atcagttttt taggtctagg gattatttca ttattccaac
    20161 aagttgcagc tggtgtaatg tctatgggag cacctacttg ggatataaca gaagtattgg
    20221 ctaatttagg ttttggatgg gtagcgtttg ttattttaat acttgcatct tggtctacaa
    20281 acgtatcaaa tgcttattct ggtggattag cattaaaaac tatttttcca aatgtaaaaa
    20341 gaaatgtttt gactttagtt gcaggtctaa taggtacaat aatagcagct actggtataa
    20401 tatttaaatt tcagtcattt ttatctttct tgggaatcgc agttcctgca atagcaggaa
    20461 ttatgtggtg tgagtattat tttatacaag gaagaactta taagcataga gaaggaataa
    20521 attggattgc agtaataagt tggttaatag gttttgcagc gtcgtattat agttcaaaaa
    20581 taaatttctt aatacctccg ataaatggaa ttgtagtgag tatggttatt tattatatat
    20641 taatgaattg ttttggaatc aaagataaat aaagggggca attacatggg tcgtaaggtg
    20701 aaaataggta taattcaaca acacagtgta cttggaaatg ttaaaaaaaa tatagaaaaa
    20761 gcagtagaga tgatagatga cttaggaaaa caaggggcag atataatttg tttaccagaa
    20821 ctatttgcta caggatataa tttagagtct ttaggtggag ttaaaacatt agaattaata
    20881 agagaacata ataagtacat agaggaatca atgtcagaag ctgctaaaag aaataatgta
    20941 tacctgatat ctccatatgg aaccttagaa aaagggtcta cacatgttta taattcagca
    21001 gttatatttg atagaaaagg taagattatg ggagaatact gtaaaaatca tctgtggtca
    21061 ttagaagcag tatactttaa aggaggggaa aaagtagagg tatatgatgc tgattttggt
    21121 agatttggtg taatgatatg ttatgatgca ggttttccag aagtatccag agaattgaca
    21181 ttaaaaggtt ctgaaataat atttatacca tctgcatgga gaatacaaga tgaagatatg
    21241 tgggatttaa atgtatctca aagagcttta gagaacactg tatatacagt aggtgtaaat
    21301 ttagtaagta atgactctaa tttaatacta tttggaaaga gtaaaatatg caatcctaga
    21361 ggtactgtaa taactcaatt agatacatac agggaaaaat atgtattatc agagattgat
    21421 ttagatgaaa taaaaaagta tagggaagat atcccctatt tgaaagacag aaaaagtagc
    21481 tataacatat aattctatac tataatacaa gttcaatata aaatgataaa aagaggatgt
    21541 atcaatatta agattaaatt tattatactt tgatatatcc tctttaatat tatcaacttt
    21601 gatttaaata tttttaaaat agttgataag aaagtttttg aacaacaaca cagctttaga
    21661 ttcatagttt tcgctaaacc atgaaagatt taaatatctt ttaaattcaa tatctttgat
    21721 atgtaacaac ttaatctcag ggtcttgaga taatccccat gattttccac atataaatcc
    21781 aactccttga agtgatttta taagagcata aattgtatat ggtgaatcag actcaaatgc
    21841 tattttgggt ttgaaattag ctgattcgca caggatatca attacctctc tataattctc
    21901 acccttcgtt ataactataa agttttcatc tgaaacttcg cttagatata cttcatcctt
    21961 taatgataaa ggatggttta ttgaaactcc taatagtatt tcttcgcatg ttaaagttat
    22021 gctattttct gagtttagct ttgtaaatga agaagatatg tacaaatcaa aatcactttt
    22081 tttataagat gatggcaaag tatgtgacac attaaaagta atattgggat atagttttct
    22141 gaaattactc aaaagtgatg gtaacacatt agccgctgca ggagataata tctttacttc
    22201 tcctgaattt tccaaagaag tgtcttgtaa ctcctttttt gaactttcta ctaaatctaa
    22261 aatgctatct actcttttta gaaacaatct tccatactca tttaaaacaa tatatcttcc
    22321 ttttctgtca aataactgaa ctccaagttc tttttctaag gaagatatag ttttgcttaa
    22381 agctggctgt gctatattta acttgtgtga tgcttttgtt atgtgctcca ttctagcaac
    22441 agtttgaaaa tacttaagat gcaataaatc catagtataa aatacacctc ttttaatatg
    22501 acttgagtta tatcaatttt ataatcaact ccacattata cattataata taaagtttat
    22561 ataataaact acaatatatt aaaaagttat ttaaattgtt atttatattt tgtacataat
    22621 ctaattaaaa aaagtaactt taaatggtta actaaggaaa tattgttaca aactttagtt
    22681 atataaaaaa ttcgtttgat gagcaaagta ttaatgtcaa gtaagaataa aagcttatta
    22741 gataagaaaa ataatatttt ataataatga tgttacttaa attattatgt tatactattt
    22801 taaataggaa acatttagat actataattt tagggggagt caagatgaaa ttaactaaat
    22861 caatatcatc aatgctgtgc ttatctctag ttttaagctt atcaccagta gcatatgcaa
    22921 atgaaataag tagaacaaca gttgagaata ctttagtagg tcaggataga tatcaaactg
    22981 ctgttgaagt aagtaaaaaa ggatggagta gtgcaaatga ggctgtagtt gtaaatagtg
    23041 aatctattgt agatgcatta gctgtaacac catttgctaa attaaaaaat gctcctatat
    23101 tattgacaga aaaaaataat ctaaattcac aaactgagaa agaattaaaa agattaggag
    23161 ttaaaaaagt ttatataatt ggtctatcta gttctgtatc aaatgatgtg caaaaacagt
    23221 tagaatctaa taatttaact actgatagag ttggtggtag tgatagatac caaacagctt
    23281 tgagtatagc taaaaaaata gaaggattaa aagatatatc agaaatagct gtagtaaatg
    23341 ggtacacagg tcttgcagat gcagttagta tagcttctgt agctgctact aatggaatgg
    23401 ttatacttcc agtatctgat gcatctggta tatctagctt taaagatttt atagaatcta
    23461 agaatataaa taaatcatac ataataggct ctactaatgc tgtatcagat aaaataaaac
    23521 aaagtttacc taattcagaa agaataggtg gagcagatag aaatgaaact aatggtaaag
    23581 taatagaaaa gttttataca tctaataaat taaacaatgt atttgtggct aaggatggaa
    23641 tgaaaaatca aaatcaactt atagacgctt tagcagtagg agtactagca tcaaaagaaa
    23701 attctcctgt actaatcgta ggagaacaat taagtgcaac acaaaagagt gtattttcga
    23761 ataaaaatgc tacctctata actcaagttg gtagtggagg aaatgaaaat gcatttaaag
    23821 aattaaaaga tttacaggca aatagtaaca ataagagagt aatgtatgta actaatacag
    23881 ataaggtaaa tataagaaat gatgctacaa tagaagcttc tgttatagga tatctaaata
    23941 atggtgatga agtagaagtt ttagatgtgt taaaaactgg atgggtagaa ataaaataca
    24001 atgaaggtat tggatatgtt agtgggtctt atttgacaaa taataagcct gataatagta
    24061 atgaaaatat aaaaataaag tatgtaaaag aaaaagatgg tctaaatgtt aggaaaggac
    24121 caagtacaga agatgaaaaa ataggtcacc tttcatatgg aagcaaagta gaaacaatag
    24181 aaatgtttgc tactggatgg gtaaaaataa agtataatgg tggatatggt tatgtaagta
    24241 atgattactt atctgataca ccagttattt aattataagt aacaataaaa aaactgcttt
    24301 aatttgtaaa gcagtttttt tataaaaatt taagtctaat aactatttct attttgatac
    24361 cttaatcctg ggatttatta ttatggatat tatcaacata caaacactcc aaatccagtg
    24421 tctaattcca aacttagata ttaataaatt cagtcctgct atggtaaata agcagtattt
    24481 aattacaatt aaaagtgtat ttatagaaag ccccttcata gaaagtatcc taaatccaat
    24541 aatagacatt ctaagtccta taaatgccaa taatatacct gcaatatcct gaattaagta
    24601 atagtattca aatttcatat tcaatcaact cttctctgag cagtatcaaa tatttctaac
    24661 tcttcaattt catcatctgt gtatgtaagc atagtaaaac cagttacacc ataaataaat
    24721 gaaattatag gtgtaatcca acttaagaaa caatatggaa taaattctag tgggcttaca
    24781 ccaagagtct gagcacagaa aacagcattt gaattccatg gtattaatgg accaccaagt
    24841 gtagctgtat cttctataat tctagataga ttttgtggtt ttaatttaaa ttctttgtat
    24901 attggagaca ttagagttcc gacaaataca gaagtgaaca tcattgatga actaaatgca
    24961 tttgcaaagt aacttactat aaaagtagac ccaaccaatt tagttcttga ttttcctatt
    25021 ttagttataa gaggttgaag cagtacttct aagaatccag ttttttggag aataccagca
    25081 ataacgaaaa ctacaaatac tagtaaaacg gtttcagcca tactcattat tccaccacga
    25141 tttaatagtt tgtcggcata gacaaatcca gtatctatag taaatcctga tagcatgtaa
    25201 tttaaaacat cacctatttt ttctccttct tgaaatactg ctataataag tcccatgata
    25261 gctgaactta gtatagatat aatgggtggc ttttgtagca ataaaagtaa taataaaaat
    25321 attattggaa tcattgctac taaaccaata tgaaagtttg aatttaatac atccttgatt
    25381 tgatttattt gaacatagtc tatagtatta tttgaatact taaatccaat tactgtatac
    25441 agaactatac atattacgta tgcaggtcct gtggtataaa acatatgctt catatgtgta
    25501 attacatctg tcttacaaac tgctgcagct aagtttgtag aatctgatag aggtgacatc
    25561 ttatctccaa agaaagcacc agaaatgact gcaccagctg ttaaaccaag aggaataccc
    25621 atactagtac caatactcat cattgctata ccagaagttc caagagtccc ccaagatgta
    25681 ccagtagcaa cagaagttaa tgaacatacg attaatgttg taagcagaaa atatttaggg
    25741 gttattattg ttagtccact atatactact gttggtattg taccagcagc tatccaagct
    25801 gctattagta caccaacaga aagtataatt aagtttgatt gaaaggcatc tttaccaact
    25861 tcaaatccaa aagcttctat ttcatcatta gtataaccaa gtttcatagc tgcaggaact
    25921 actataagcc atgaaagtaa aaacattgta gttatagaag cattaaatac aactatacca
    25981 actgatgtta gagctattat tgcaagcatt actaacaagg aatatctaaa tgaaggtatt
    26041 tttctttctg tattcatttt catagtgcaa attctcctta aatattttta gttaattgca
    26101 ttttttattc ttataagagc ttcttcaagc atacttcttg gacaagctaa gtttattctt
    26161 tgatacccac ttcctccaat gccaaagctg tttccttgat ttagagcaac tttacctttt
    26221 tgtacaagta tactttctag ttcctcatca cttaatccaa gtgcactaaa gtctacccat
    26281 aataaataag taccttctgg ttttctaact ttaagttttg gcatattttc atttatatat
    26341 tttatagcaa aatctatatt agattctaaa tattctaaaa atgattctag ccaagattca
    26401 ccattattat atgaagcttc agttgcaact aagctaaagc aattatttct ctttatatca
    26461 attctggtaa aggcatcatc taacaattta tagtcttttt catcaggtag tattacatat
    26521 gatgattgaa gaccagctat attaaaagtt ttagttggag ccatacaagt aattgtattt
    26581 ttttcaaatt ctttagaaat agatgccata ggaatatgct tatgtttttt taatataatg
    26641 tcactatgta tttcatcaga tatgattttt acattatgct taagacaaat atctcctagt
    26701 ttttttaatt catcttttgt ccatactcta ccaactggat tatgaggatt acaaagtatg
    26761 aataatttta catctttaat cttgttttca atgtcttcat aatccattat gtagtttcca
    26821 ttttctagtt tttgtaatgg gcttataatt agttctctat tgttattttt tacaacacta
    26881 ttaaaagggc tgtaaactgg ttcttgtatc attattttat catttgcttt tgtgagttca
    26941 tttattaaaa gacttatagc tgggataaca cctggactat aaattaacca ctcacttttt
    27001 attttccagt tatgtctcct atctagccag tttactatag attcattgta agaatctggt
    27061 cttgttgtgt agccataaat ctcttgttct agtctatttt ttaaggaatc aattatacaa
    27121 ggtgcagcct taaaatccat gtcagccacc cacataggaa gtaaatcatt agttccatac
    27181 tttttttcca tttctgacca tttagatgaa aaattattgc ttctatcaac aatctcatta
    27241 aaattataat tcattgtaaa tacctccagt aattttaatt ttatgttaat aatattaagc
    27301 aaacaacgtg ccaatattga taataaaaaa tatttattca caagttaata tttaatatta
    27361 agtaatttca gagcatagat aagataaaaa tattaattat acttaaaact ttgtggaaaa
    27421 ggttatttct aataaatttt taaatatatg aaaatgtaat taaataggtt aaataggttt
    27481 aactgtttaa cttgttagac ctatttatat tttttagaaa ttctactcct tttgatgtta
    27541 taattgaacc tccacgacca acaagtatgt ctacaagccc aaagttttgt agttctaaaa
    27601 gcatatttct aatctgttgt tctgtcaaaa atctattttc ctctaaagct attttacata
    27661 agctttttct acctattctt tcttttaaat cataagcctt ttttaaatta tccaatataa
    27721 atatgtattc atctagtgac cttttaaaat tatagtcgta tttcatgatg tttttgtctt
    27781 tgccaatgtt gaattgatac ttttttatat tatcacttac tttattacaa acggttctac
    27841 tataatttga atctatagta tctaatatat attcaggtaa atcacatatt tctataatat
    27901 ctttttccaa gtaacagaag tattctccta agtttcttaa ttctcttaca tttccttccc
    27961 atctatacat tttaaagatt tcttttactt cctcacttag aataaaatta catggaatat
    28021 catattttaa tgaaccaaaa atttcaaata tatcagcagg tctttcccta agtggatgta
    28081 ttttaagggg aagaacattc aacctaaaat ataaatctct tctaaacata tttttagata
    28141 caagttcttt taaatttcta ttagtagctg caattattct tacatcaatg tcaatgacat
    28201 tattagaacc aatacgtcgt acttgttttt cttgtatgac tctaagtaat ttagattgag
    28261 aatttaaatc catttctcca atttcgtcta aaaatatagt tccattgttg gctaattcaa
    28321 ataaaccaat tttcccacct tttttagcac cagtaaaagc cccttcatca tagccaaata
    28381 attcactttg cagtagattt tcttgaaaag tactacaatt tactgcgaca aaaggaccat
    28441 cttttctcct agaagcacta tgaatggatt gagcaaataa ttctttacca gtaccacttt
    28501 caccaattat aaggatagat gaatttgatt gagccatttt attagcaatt ttttttgtat
    28561 taatagtttg tatactagaa cctaatatgt catcaaatgt gtatttactg atattaccag
    28621 agtttactag ttgtgctctt aatttagctt gctttttttc taattgagaa aactttgtca
    28681 tctttagtat aaatccatca aatacattta attgtatatg tttaatctct aaatttatat
    28741 caatatgatt tattttaatc aatttctcaa aaaatggagt tttattggtc aatatgcttt
    28801 gaaaattgat gtcttttatg caatcaccaa caaatgagtc aatcatttca tttgcattta
    28861 tagacaaaat ttttctagcc atatgattat agaattgaat tattccatcg ttatttgtgc
    28921 atataattcc atcatcaatt atagatagca acaaatcaaa ttgtctttca aacttatttg
    28981 catctaacaa taactcacct gtactataac ttgtaggaat tattttttct acgtattttt
    29041 ttattaggtc atcttttatt agatgtttta atttggtttt tgtagcaatt tcaacaatac
    29101 aacttaaatc tattattctg tattttaaat ctataacttc acaattttta aacttaggca
    29161 agattttttc gccaggagtt aggattacag catctgtggg agtaaactca atctctggat
    29221 agcagggcag gatatttata ttgtctattc ctaatctatg tattaaagaa attgtttcta
    29281 ttgccatatc tttacttaag ttatatacat aaataatttt atctttttct aatttggata
    29341 tcatatcaat actgttcttt tcaaatgtca acttaggaac aatgatttga gcatcatagt
    29401 gagataattt tactatttca tcatatttta aataagcaga taggagtata agtttttctt
    29461 taaaaaactt taaatttccc tcttcaaatg agtataaatt tatctttaga ttatcaccta
    29521 agaattgcct tatttgattt tcataaattt ctccagcgtc tttttttaaa cttacaatag
    29581 ctaattccat ttgtttcatt ttaacctctc atacaaattt gacttatttt agattacata
    29641 ataattaaat tagattaaat aatattattt aattattatt ataaatttat tatagactac
    29701 acattgaagt aaaagaagta gctaaaatta ttccttttat ttttgttttt tgtatctatt
    29761 tggtataata gtattagagc gtataataat ttaggcaaaa ttttttaagg aagactagag
    29821 gagaatataa aatgaaaaaa tgtctttact gtaataggga tataaagtca ccaaataata
    29881 aattagctat aaaatatgat gacaatatga atatatatcc atgtcgtgca gaatgtaagg
    29941 aaaaaattga agaatatata gcaaaaaaac ctacatataa gtttataaat atattagagg
    30001 ttcttttagg tgtgggcgga atattctgct ttttgagtaa atggactatt gcagcccaag
    30061 ttgtacttgg aattgatgca ctggttatat ttttattccc attggcagga tttaaaatga
    30121 atggaaagtt aagtatggaa caaactaaga atcaaagtag gtctattgca atatcaattt
    30181 tggcatttat agtaataata actgtattat attttaaaca agttggtaag tagagcataa
    30241 ttgtataaga catcattaga gtatgaggtg agtttatgga agaaagaaaa ttagcaccaa
    30301 acaatgagag ggaagctctc ataaaaagat taaaaagaat agaaggtcaa gttaagggta
    30361 tacaaaagat ggtggaacaa gaacgttatt gtgttgatat attggttcaa atatctgcta
    30421 taaggtctgc tataaataga gtaggcacaa ttatcttaga gaatcatata aaaggatgtg
    30481 tagcagaaag tattaaacat gatgatgctg ataaaactga agagatgata aaagagctta
    30541 tggatactat aaataaattt acaaaataag gaggcggtta gttatgaatc aacaaggatt
    30601 tattatggaa attgttgatg atagaactgc aaagttaaaa atgcaaagac attcagcttg
    30661 tgctgcttgt ggaaaatgtg ctactacaaa ttcagcagag tcaaaagaaa tattagtaga
    30721 agtagacaac acaattggtg caaaagttgg tgaccatgta gaagtgagta tggataatat
    30781 gaatgtatta aaagcagctg taatggctta tataatacct cttatagcac ttcttgtagg
    30841 aacaataggg acttattata tattgggggc aattggaatg acaactggag ttgaagccat
    30901 aagtggatta gttggaattt gtcttacaat aatctgttat ttatacttaa agaagaatga
    30961 caaaaaattt agagacagta gagaatttat accagtaata acaagaatat taattgacct
    31021 ttaagataat tataattaaa aaatagcaat gtacttttta aataaatact caatacgtat
    31081 ttgtagagtt taagtacatt gctattttta tatttttatt ttgattttct gtaaggaata
    31141 aagttttttc tgatttttaa aagattcaat gttcttacaa aagaagttac aactaataca
    31201 ccaagtgcta ttgcagcact tagaaatagc gcatctgtac cttttgacat agccaaagaa
    31261 taatttttct gaatcaaaga caacattgta ttatatagcc caattcctgg tattagagga
    31321 agaatacctg gaattacgaa cacaatagct ggttgcttta gttttcttgc taaagtctca
    31381 ctgcacgctg agaccaaagc agcagcgata aaacctgcaa aaactgcatt tgtagtaaaa
    31441 ttaaataaat aaacatatac aatccaacca attccgccag ttactccagc tggaagaagt
    31501 agatatagtg gagcattaaa gaaaacagca aatccagcag ttgcaacacc tgaaaataaa
    31561 aaatgcaaaa tcatagacat atcagtcatt agaaaggacc tcctattcta taccatgtat
    31621 caaggacaaa accaactcca gaagcaattg caataataat catagcagca tcaaaagcac
    31681 gagaaattcc tgatattaaa ttccctgata ttaaatctct aatagctttt ataaaagcaa
    31741 ctcctgggag tagtggcatt atagcgccga ctataagcat tttagggtca tctaaaatgc
    31801 ctatttcaac tagtagaact cctgaaagtg caattaaaaa agaagatact aaacaggaaa
    31861 atgcagatat tgcacttatc ttcatcgttt tatcataagt aattgcagca aagattgcta
    31921 ttataaacgt aagtataaaa tttaatacat aattccctcc aagcaaacaa gcaaaacaag
    31981 cagatgcaat tcctgtacaa gagtaaaata accataaagg ataaggtttt acatcttgga
    32041 tttcataaag acgtgcaatg gcattatcaa tatcatactc tttgtcacta acaaattctc
    32101 gagaaaaact gtttaataaa gaaatcttat ttaagtttat acctcttgat tgaatcgttt
    32161 tcataaacga aaagccatca aatttctcat ctgaaataat tattactgta ggagttgtaa
    32221 aaatatttac atgataaaac cctcttgacc tgcatattct aaggacactg tcttcaaccc
    32281 tagatgtttc agctccattt tttatcatca gttcccctac aaatagagct agttttagaa
    32341 tatttttctt atattcaagt tcctcatttt gattcataaa tacacctcca aattaaaaaa
    32401 cgtaatattg aaattatatc aaatttttaa gtaatattcc ttaatatcta tacaaatata
    32461 ttatcttttt tttgtttcaa aatagtcaca tttaaattat atccaattaa aagaattaga
    32521 caatattatt tattatattt taacttaaaa ataaagaaag taactatgtt aaatatggaa
    32581 aaatggcaaa acacttgact attaattggt aaaggactat aataataaat tatcatattg
    32641 ataaaaacga aaattatgaa atgtctgtaa aaataaaatt taaataataa ctaggaggag
    32701 taatatgttt gagaacttga aaaaagcgga ccctgagatt tatgaaagta tgaaaagaga
    32761 gttaaaaaga caacaaagaa atattgaatt aatagcatca gaaaacatag tatcagttcc
    32821 agtaatggaa actatgggaa gtcatcttac aaataagtat gcagaaggtt actcaggcaa
    32881 aagatattat ggtggatgtg aatacataga tgaagttgaa actcttgcaa tagatagaat
    32941 caaaaaaata tttggagcag aacatgctaa tgttcaacct cactctggag caaatgcaaa
    33001 tattggtgta tattttgcta tgttaaaacc aggtgatgtt gttatgggaa tgaatttatc
    33061 tcaaggtgga catttaactc atggtgctcc agttaatatt tctggtcaat attataaatt
    33121 ttatgaatat ggtatagata aagacagtgg acttatagat tttgatgaag ttagaaagat
    33181 agcacatgaa gtaaaaccta aaatgatagt tgcaggagca agtgcatatc ctagagaaat
    33241 agattttaaa aagtttagag agatagctga tgaggttgga gcattgctta tggtagatat
    33301 ggctcatata gcaggtcttg tggctgctgg tcttcaccaa aatccatgtg aagtggctga
    33361 ttttgttaca acaacaactc acaaaacttt aagaggacct cgagggggtg ttattctttg
    33421 taaagaaaaa tatgctaaag acatagacaa agcaatattc cctggtattc aaggtggacc
    33481 tcttgaacat ataattgctt ctaaggcagt ttgctttaaa gaagctttaa gtgatgagtt
    33541 taaagaatat caggtacaag ttgctaaaaa tgcaaaagcc ttagctgaag aacttattaa
    33601 aagagatttt aaattgattt ctggaggtac agataatcat ttaatactat tagatttgac
    33661 taataaaaat ataactggta aagcagcaga aaagagattg gatgatgctt atataacagc
    33721 taataaaaat acaattccat ttgacccaaa tggtgctctt attacaagtg gaataagact
    33781 tggtactcca gctgtaacta ctagaggtat gaaagaagaa gatatggcaa taatcgctga
    33841 ggctatagac ctatgtttaa cttatgatga agaatctaag gctagaacat tggttgttgg
    33901 tttaactgaa aaatatcctt tatatgaaga atataatatt atggattaat tataaagaac
    33961 aactaaataa aatacattta aataattaca atgattgtgt atgtatttga atatagttag
    34021 atataaaaaa ggtgtctcaa aatgaacttt ttagtttatg aggcactttt ttgtatggaa
    34081 tcgaacatat ttgtgttatt taaataataa aaaatatggt tatctctatt tttagatggt
    34141 ctctttgtat tttttaatta aaactttaag atttcttaag aattatacta ggtttttcta
    34201 aagatttaaa gtctatactt ataagtatag taatcatatg aaaagaaaag ctttaacgag
    34261 gagggaatct tatgaaggta ttaattctta caggaaaatt tggtatgggt cattactcag
    34321 catcaaattc tttaagtgaa gatataaaag caaaatttga taattcagaa ataataatta
    34381 aagatatatt tgagtacatt atgcctaact attcagataa aatgtacaag actttttcta
    34441 ttcttgtaaa tcggggaagt agtctttata atttatttta taaatgtgct gaaaatggaa
    34501 agaaagatat aaaatttact ttttcagatt attttttaaa taaattagat acattacttc
    34561 atgaagtaca accaacagta gttatttcaa cttttccatt ttgttctcaa ttagtatcaa
    34621 gatataagga aaaatataac tcaaatcttc ctttaataac atgtataact gatataagta
    34681 gtcactcaga atggattagt aaaaatacag actgttattt agtagctagt aaatctacta
    34741 aggaagaact tgtttttaaa ggaatagatg aatctaagat taaagttaat ggtattccag
    34801 taaaaaaaga atttaaaaga atagaacatg taaatcattc aactaaaaaa aatattctta
    34861 taatgggtgg aggattagga ttactgccta aatcagaaca attttacaaa gaattaaata
    34921 gtttagaagg agtaaaaact actgtaataa ctggtaataa taaaaaaatg tactataaac
    34981 tatatggtag atatgaaaat atagaggtag taggttacac caatgaagta tataaatata
    35041 tgaaagattc tgaccttatt atttctaaac caggtggaat aacactattt gaaacaattt
    35101 attcagaact tccaatattg gcatttaatc catttttaca acaagagata gataatgcta
    35161 gttttatatt aaataatgaa attgggagaa tacttggtaa aaataaaaaa tactatgtag
    35221 atgagataaa agatttaatt tatgatgatg ctactcttaa agaaatgagc agtaacatga
    35281 aagaattaaa aaaacaattt gataataata ccttagagaa tattttattt tcacttgatg
    35341 aacaaggagc gtgtagagaa tgtatgtagt aggtttaatt atcgttgttg cattgatttt
    35401 tttagtccat tcaattatac ctacatacta taataaacta ttaaataaag aggtattaaa
    35461 aaatatggct ggagaaaatg aaattgcact tacatttgat gatggtccag ataagagata
    35521 tacagaaaaa ttactagatg tgttaaaaga aaatgatata caagctatgt tttttgtagt
    35581 agcaaagaat gctgaaaaag agcctgaaat tataaaaaga atgttgagag aaaatcatat
    35641 agttggttta cattcattgg aacacagaaa tgcatggctt tatagttata gttatgttaa
    35701 aaaagatttt attgaaagta caaatataat gaaaaattta ggtgtagatg taaactacta
    35761 tcgtccacca tggggtcata ctaatatatt ttcaaattca tttgttaaaa aatataatct
    35821 aaaaatgact ttgtgggatg taatggcaga agattgggag aaagatagta cagtagacat
    35881 tattataaac aagttaatga gtagaacaaa agaaaattct ataatttgtt tacatgatgc
    35941 aggtgaaaat tcaggtggtg cagtaggtgc acctgagcgt actattgaag ctttaaagat
    36001 agctattcca aaacttaaag ccagtggttt aaagttcgta acaccagaaa ggatgtagtt
    36061 atgataagta aaactgaaaa gaaaaataaa tgcctaggta gtgtagcttt tttagtttta
    36121 ctaatgggca taacaggata ttttgttttt agagggcagt cagttgaatc attgataaaa
    36181 tcattaaaag gtgcaagccc tatgtttata ctaattggat ttgcaatgat gtttatatat
    36241 gttgcctgtg aaggtataaa tatatactta ggtatgaaag ctttaaacca aaagactact
    36301 cttttaaaat gtatgggata tgcttttatt ggattttatt ttagttctat tacaccttca
    36361 gcatcaggtg gtcaaccagc acaagtttat tatatgaaaa aagacgatat aaatatatca
    36421 tattcttcac ttatactatt agtcattgtt gttattcacc aagtagttat actggcatat
    36481 agtggaataa tgttcattat ggaacgtgag tttatattaa ataatgtaag tggtatgaac
    36541 attttactaa tatatggtgt aataacaaat gttgctcttg tcattggagt aattgcaatt
    36601 atattttcaa aaaaacttgt aaacaatttt ataatatcaa taacaaattt attaggtaag
    36661 ttaagaataa taaaagatgt tgaaagttca agaaaagtta taatatctca aattgaagag
    36721 tatgttaaag gtgctcaata tataaagcag aatccaaaac ttgtagtcca aattttagtc
    36781 ataactattg tacaaataac agctatgttt ttagttcctt tctttgtata caaagctttt
    36841 catctatcaa catatactgt atttgagata ttagcgatac aatccttgct taatattgct
    36901 gtttcttcat taccattgcc aggggctgtt ggagcttctg aaaatagttt tatgacatta
    36961 tttaagatat ttttccctgg tcacttatta gttccggcaa tgttacttag taggggaata
    37021 agtttttatg catttgtagc tataagtgga ttgatatgta tagtagtaca tgtaaaatct
    37081 tctgaaaaag taaaagcagt gaaaaaagtt gcttatgtaa gataatatta tatagattat
    37141 ttttattaat atgtcaattt gtgtgtatat tttaattaag ctttaaacat atttacagga
    37201 gtagaaacta ttaaaacgtg tgacattgct aagcttttgc taataaagta taatatttta
    37261 tatagtttga aatatttaag aaatatagca aaaaaaggag cttggtgatg aataaagaga
    37321 ttccaaaaaa aattttagta gctgatgata atcctgagat tagggaaatc gtagaaatac
    37381 ttttaactgg tgaaggttat gaagttatta tggcaacgaa tggagaagaa gctgtaaact
    37441 tagtagatag tacgattgat ttgattattt tggatgttgt aatgcctgta aaaacaggat
    37501 ttgttgcatg taatgaaatt agagaaaaaa cgacagcacc aatattattt ttaactgcaa
    37561 aaactcaaga ctctgataaa gttattggat tttcagcagg aggagatgac tatttatcaa
    37621 agcctttttc atattctgaa ctaatttcaa gagttaagtc tctacttaga cgttattata
    37681 tataccaggg aaaggaaaag gaaagagagc caaatatatt aaaaacaaaa gagttatgtg
    37741 tcaatttaga tactcaagat gttacactaa atggagaacc tgtgtttttg acaactatag
    37801 aatatagtat attaattctt atgttgaaaa atagaaaaaa agttttttcg tctgaaaatt
    37861 tatatgaaac tatttgggat gagcagtatt tttatacagc aaataataca ataatggttc
    37921 atattagaaa tttaagaaaa aagttagaaa aagaccaaaa aaatccacaa tatataaaaa
    37981 ccgcatgggg aaaggggtat tatattgatt aacaaaatta agccatctaa gttgggagtt
    38041 atactattga tatatgtagt aatctcagct gcagtggctt tcattttaaa tataccattt
    38101 tactctgctg ttgaatctat agtaaaagat gtatcagaag ggaaagtata ttttaaaaga
    38161 gaagtaaatc aacttatgga taattttcaa acttatgtga ataacaatgg tataagagca
    38221 gatgatggag atgatatagc aaaatggaat agtgataatt ggtttgtttc tatgaagata
    38281 tataaaggaa ataaggtttt ttatgataca ttatactatc ctaatgaagt acctgataag
    38341 agtgaggaaa atgagtttta tagcagtccc atggctagaa actatgagat acaattttct
    38401 gatattaaag ctaatatagt tgtagtagct ttatttagat ttagatttca aaattttata
    38461 gaagcaattt ctattacatt tatggcactt acatttatta taacattttt actgttgttt
    38521 agtagaaaga taaagtacct aaggaaaata gaaatgggta taaagatagt agagagtggc
    38581 agtttaaagt atagaatacc agtaaaaggt aatgatgaat tatcatcact tgcatctagt
    38641 attaatgaga tgagtcatac attggagaat caacttataa aagaaaggga gatgaagaat
    38701 aaaaataaag atatagtaac ttcaatatca catgatgtta gaactccatt aacgtcagta
    38761 atttgttatt tagatttgat taaagaccat aaatacaata gtcctgagca attggaacaa
    38821 tacataaaaa atgtaaggtc taaagcatat caaataaaag atttaacaga tgaccttttt
    38881 acgcattttg ttaattctgg agaggaagtt tcaaaagggt atgaaaaaat aaatgctaat
    38941 gaatttattt ttcaattact tttagatagt tcatatacat tggaggagaa aggatttcat
    39001 ataacaatag aaaatgaact ttataaccaa tatcatattt atgtagatgt tacacaattt
    39061 agaaggatat ttgataattt atgttctaat ataataaaat atgctaggaa agataagcct
    39121 atagtatttt taattaaagt agaggattct aaattactaa ttatacaaaa aaatagagta
    39181 agtaattttt ctaagaatgt agaaagtttt ggaattggta taaagaattg tgagaaaata
    39241 gttgcatatc ataatggaag cataaatgta aattcagatg ataaagaatt tgctattgaa
    39301 atatctattc cagcaaaaga atcataaaaa tttaaattat atattgtgta attgctatta
    39361 tatattgtgt aattgcttat gtaaaattct atgaagcagg aagcatgtga ctttagttat
    39421 gggatgttca atattgtgta cctctaggca agtaacaagt ttacttacaa gaccatataa
    39481 tattattatc ttaaaaaaag gggacataat aatatggttt tgacaggagc acattatatt
    39541 tacattctat ttatggtaat tatattaatt actatgatta tgaaaaagga tacaatagta
    39601 ccatgtatat taggtgtttt ttttatgggg ttgttctttg aaaaaaacat ttttggtgct
    39661 ataacagcag tgtttaactc ttttattata tctttaaatg agttgggtcc aattatcttg
    39721 attatagcta ttatggttgc attatctaaa gccctagaag ccaataatgc tatacagtat
    39781 atggtgagac cattttcgag agttataaaa aattctaata cagcattttt tgtaactgga
    39841 tttgttatgt tagttctttc ttggtttttc tggccaactc cagctgttgc cttagttgga
    39901 gctgtatttt tacctgttgc tatgagagct gggctaccag caattggtgt tgcagtagca
    39961 ttaaatttat ttggtcatgg tcttgccttg tctacagact ttgttattca aggagctcca
    40021 agtataacag ctggtgcagc aggtgttgca gtatcatatg ttataaatga tggtatgatt
    40081 ttgttttggg tgatgggaat cgtaaccata tctatggcat tttatacatt aaaaagagat
    40141 attaacaaag gaatgtttag agaagaacta aaaggttttg aaagtgaaga agtagaagag
    40201 ttcaatggaa aatctaaaat agcaactatt ttagtagctc tgggatttct tgctgatata
    40261 attgctatgt atatatttga cctaaaagga ggagatgcat cagctctttt gggtggaaca
    40321 gcagtatttt taataataat tataaatgtt ataaattttg gcaaagatag tcttgaaaat
    40381 gtatgtgaga atataattga tggttttgtg tttgggataa aaatatttgg agcaattata
    40441 ccaatagcag catttttcta tatgggtgaa gttgcaccat tgacaggtgt atttggaaaa
    40501 gtattagcac caggttcaca aggtttatta tcagatatag gcatagctct ttctcaaaca
    40561 ataccactta ataaatttgc cgtatcaggt atagaaactg tagtgggagc aataactggg
    40621 cttgatggtt ctggattttc tgggatttca ttagttggtt cacttgcctc tgtatttgga
    40681 actgctataa atgcaagtgt tggtgcacta gctgcattag gacaaatcag tggtatatgg
    40741 gtaggtggag gctgtctagt tccttggggt cttatttcag cagcggcaat ttgtggagtt
    40801 agtccaatag aattggcaaa gcgaaacttt gtcccagtta taaccggatt ggttgtaaca
    40861 accatagtag caatgtttat tatttaataa atcgaataaa tctttaattg tataaattaa
    40921 aaatagatgt gtagctataa tttattataa ttgttttact aattctaagc catacatcta
    40981 tttttattga tatttttatt agtattcttt aataaaataa tatttttcat tatactatac
    41041 ataacatcta aatataacat ctaaaatact gttatctata tttaatatct tgccaaattt
    41101 tttgatgttt tattgccctg tcccaccaac atatataagc tatcacaagt ttttacctat
    41161 actatcacca aacaaaaatt ctaattttaa tttattattt tataacctat attatattaa
    41221 ctatattagt ttgtagactt tagatgtaat tataaatatt aatactccaa gagttcaaat
    41281 tcatatccta gttttcttgc atttgatata aaatcattta aaatttctgt actagttttg
    41341 gaaactgcat gtagtaacaa gatagaacca tcatgtagat tatccattat tttttgcgtt
    41401 gcttcttcat gtgatggttg tttatcagta tcccagtctc tgtatgcaaa actccaaaat
    41461 acagtcttgt atcctaaatt tttagtcatt gccaagcttt tttctgagta ttttcccata
    41521 ggaggtctaa aaaatttaac catatctttc ccagttacat ctttatataa cttttcaaca
    41581 tcatataatt cgtcattaaa gttttttaga ttgcttgttt ttgttggcat agatggatgg
    41641 gttttactat gatttccaac tatatgtccc tcagaaacca ttctttttac taaatcttta
    41701 ttttctttta tatatggagc tgtaacaaaa aaaactgctt taacctgatt ttgttttaga
    41761 gtgtcgagta tttttgtagt gtatccattt tcataacctt catcaaatgt aagatatagt
    41821 gttttttgac catcttttgt aggtccattg tatagggcat catagtcaga aaatttaaat
    41881 tcaatgtccg tatttacttc tggtgtttta tgcttattgt ttggtatata aaaccaatca
    41941 agagtagtgt tatctaaaga agatacatct aatttattat tatcattatt agtagtggct
    42001 tttgatggtt tatctgttcc gtctgaaact attacattgg aagtatcttt accactatct
    42061 attttttcct tatcttcttg taactgtgtt tccttatttt gattttcgtt ttgattgttt
    42121 tgtgaatttg aacatccaac aataaaaaaa atagaaatag aaagtagtgt taaataatgc
    42181 aatacttttc taaacatttt gaagctcctt tctaaattaa taataattgc atataataat
    42241 aattatatga ttaattacaa ttgcatatag tattattata attggaaatt actattaata
    42301 atagttatat ctttaaaaat aaggtcacaa tagtatgaca aagggtgaat aatcaataaa
    42361 tttaaaaaaa aatgaaaaaa atagttgacc atattatgag aagatgttat tataataaat
    42421 gtgctcaaca agaagtaaca aaataacaag cacaaagaag ttaattgaat gaaatgaact
    42481 ttgaaaatta aacagtaggt taatttataa aacaagaaac aaaccataaa gccagatatt
    42541 ttgataacaa tagtatctga gcctgataaa cttttatttg agagtttgat cctggctcag
    42601 gatgaacgct ggcg
//
LOCUS       DAYAOY010000027        41261 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYAOY010000027 DAYAOY010000000
VERSION     DAYAOY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.027"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..481
                     /gene="bilQ"
                     /locus_tag="ACGI6B_001527"
     CDS             <1..481
                     /gene="bilQ"
                     /locus_tag="ACGI6B_001527"
                     /inference="COORDINATES: protein motif:HMM:NF045593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HFG9750975.1"
                     /translation="KHIIIIEGKFKMDFKNLQYESLSYIICNLQKNFKLYCEKCLKPY
                     KLTNGLYFYLIYINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSR
                     AFQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            483..1577
                     /gene="bilR"
                     /locus_tag="ACGI6B_001528"
     CDS             483..1577
                     /gene="bilR"
                     /locus_tag="ACGI6B_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HFG9750976.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1665..2195
                     /gene="bilS"
                     /locus_tag="ACGI6B_001529"
     CDS             1665..2195
                     /gene="bilS"
                     /locus_tag="ACGI6B_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HFG9750977.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHVESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2546..2881
                     /locus_tag="ACGI6B_001530"
     CDS             2546..2881
                     /locus_tag="ACGI6B_001530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750978.1"
                     /translation="MPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWL
                     QIHAFYSDTIADYLTNFMCFVALSIEIFSIFFIYNIVRHVTIIIFIDILVFIALLIIG
                     VYKLFKKYN"
     gene            complement(3131..4507)
                     /locus_tag="ACGI6B_001531"
     CDS             complement(3131..4507)
                     /locus_tag="ACGI6B_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750979.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4775..5947
                     /locus_tag="ACGI6B_001532"
     CDS             4775..5947
                     /locus_tag="ACGI6B_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9750980.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            5961..6608
                     /locus_tag="ACGI6B_001533"
     CDS             5961..6608
                     /locus_tag="ACGI6B_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFG9750981.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6610..7119
                     /locus_tag="ACGI6B_001534"
     CDS             6610..7119
                     /locus_tag="ACGI6B_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFG9750982.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7483..8595)
                     /locus_tag="ACGI6B_001535"
     CDS             complement(7483..8595)
                     /locus_tag="ACGI6B_001535"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFG9750983.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8959..9159
                     /locus_tag="ACGI6B_001536"
     CDS             8959..9159
                     /locus_tag="ACGI6B_001536"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFG9750984.1"
                     /translation="MVGGGDIVKLFMKDNLIDDYYIYILTIILGRGISLFKSGIDKTN
                     LNFKKASNIGELVKLEYSKKQK"
     gene            9404..11131
                     /locus_tag="ACGI6B_001537"
     CDS             9404..11131
                     /locus_tag="ACGI6B_001537"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750985.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11131..12921
                     /locus_tag="ACGI6B_001538"
     CDS             11131..12921
                     /locus_tag="ACGI6B_001538"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9750986.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13083..13391)
                     /locus_tag="ACGI6B_001539"
     CDS             complement(13083..13391)
                     /locus_tag="ACGI6B_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750987.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13653..14201)
                     /locus_tag="ACGI6B_001540"
     CDS             complement(13653..14201)
                     /locus_tag="ACGI6B_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750988.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14304..14975)
                     /locus_tag="ACGI6B_001541"
     CDS             complement(14304..14975)
                     /locus_tag="ACGI6B_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9750989.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15368..16276
                     /gene="garR"
                     /locus_tag="ACGI6B_001542"
                     /pseudo
     CDS             15368..16276
                     /gene="garR"
                     /locus_tag="ACGI6B_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            16412..17794
                     /locus_tag="ACGI6B_001543"
     CDS             16412..17794
                     /locus_tag="ACGI6B_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HFG9750990.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17865..19301
                     /locus_tag="ACGI6B_001544"
     CDS             17865..19301
                     /locus_tag="ACGI6B_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFG9750991.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19487..20341)
                     /locus_tag="ACGI6B_001545"
     CDS             complement(19487..20341)
                     /locus_tag="ACGI6B_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFG9750992.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20609..20932)
                     /locus_tag="ACGI6B_001546"
     CDS             complement(20609..20932)
                     /locus_tag="ACGI6B_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFG9750993.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21135..21764
                     /locus_tag="ACGI6B_001547"
     CDS             21135..21764
                     /locus_tag="ACGI6B_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9750994.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22076..22585
                     /gene="ruvC"
                     /locus_tag="ACGI6B_001548"
     CDS             22076..22585
                     /gene="ruvC"
                     /locus_tag="ACGI6B_001548"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFG9750995.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22578..23180
                     /gene="ruvA"
                     /locus_tag="ACGI6B_001549"
     CDS             22578..23180
                     /gene="ruvA"
                     /locus_tag="ACGI6B_001549"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFG9750996.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23287..24306
                     /gene="ruvB"
                     /locus_tag="ACGI6B_001550"
     CDS             23287..24306
                     /gene="ruvB"
                     /locus_tag="ACGI6B_001550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFG9750997.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24624..25649
                     /gene="queA"
                     /locus_tag="ACGI6B_001551"
     CDS             24624..25649
                     /gene="queA"
                     /locus_tag="ACGI6B_001551"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFG9750998.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25661..26146
                     /locus_tag="ACGI6B_001552"
     CDS             25661..26146
                     /locus_tag="ACGI6B_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HFG9750999.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26225..27346
                     /gene="tgt"
                     /locus_tag="ACGI6B_001553"
     CDS             26225..27346
                     /gene="tgt"
                     /locus_tag="ACGI6B_001553"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFG9751000.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27480..27773
                     /gene="yajC"
                     /locus_tag="ACGI6B_001554"
     CDS             27480..27773
                     /gene="yajC"
                     /locus_tag="ACGI6B_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFG9751001.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27831..28193
                     /locus_tag="ACGI6B_001555"
     CDS             27831..28193
                     /locus_tag="ACGI6B_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HFG9751002.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28259..28402
                     /gene="scfA"
                     /locus_tag="ACGI6B_001556"
     CDS             28259..28402
                     /gene="scfA"
                     /locus_tag="ACGI6B_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HFG9751003.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            29028..30923
                     /locus_tag="ACGI6B_001557"
     CDS             29028..30923
                     /locus_tag="ACGI6B_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HFG9751004.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            31385..32788
                     /locus_tag="ACGI6B_001558"
     CDS             31385..32788
                     /locus_tag="ACGI6B_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HFG9751005.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      32960..33045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            33169..39132
                     /locus_tag="ACGI6B_001559"
     CDS             33169..39132
                     /locus_tag="ACGI6B_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HFG9751006.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            39342..41222
                     /locus_tag="ACGI6B_001560"
     CDS             39342..41222
                     /locus_tag="ACGI6B_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HFG9751007.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
BASE COUNT        16240 a         4704 c         7209 g        13108 t
ORIGIN      
        1 caaacatatt ataattatag aagggaaatt taagatggat tttaaaaatt tacagtatga
       61 aagtttgtca tatattattt gtaacttgca aaaaaacttt aaactttatt gtgaaaaatg
      121 tttaaaacca tataaactta cgaatggtct atatttctat ttaatttata ttaataaaaa
      181 taggaattgt tctttgaatg atgtttctac agaatttgaa gttgataaag ctcatacaac
      241 aagaactata tctaggttag aacaagatgg atacatagaa aaaatccaaa accctaatga
      301 tagtagagct tttcagttga gagtaacaga taagggtgag gaagtattag gggatataaa
      361 aaacatattt tcaaagtggg ataatcacat aaaaaaagag ttctcggata cagaatataa
      421 agaactggta aagaatttac atgtggtaaa ggatataaaa acagctgtgg aggaagaata
      481 ggatgtattg taatttatta aaaccaatta aaattggaaa tttggaatta aaaaatagag
      541 tatcttttgc acctacaagc atgggtctta aattggaaga aaaaattaaa aagttttctg
      601 atattgctaa gtcaggagta gctctgatta ctctaggaga tgtatctatt agacctagtt
      661 ttcataaggt agctataagc ctatcggatg aagatggagt catgaaatac aagaaaattg
      721 tagatgaaat acacaattct ggtgcaaagg tatcagcaca actattttgt tcagattatg
      781 atgttaattt aattaaagat actatgaata tgggaattac ttcacatgat gagataaaaa
      841 aaataatgaa tgacggagta aaagattaca taacaaatat gcccaaagaa gaaattaaaa
      901 atattataaa tttatttaaa gtaacagcat tgaatgcaaa aaaagcaggt tttgacatga
      961 ttcaaattca tggtgatagg cttgtaggaa gtttttcatc aagtatattt aataatagaa
     1021 atgatgaata tggtagaacg tgtgataata gaagtagatt tgcaagcgaa ataattagta
     1081 gtatccgaga tgaagttaag gacattccaa tagactataa atttgcaata agacaagaaa
     1141 atcctcatta tggaaatgca ggagttttac tttcggaagt tgagtatttt gttaaaaaat
     1201 ttgaatccct aggtgtaaac agttttcatg taacacttgc aaatcattca aaattagaag
     1261 atactatacc aaccaataat catccttact ttaaagaaga aggctgtttt ttatatttag
     1321 ctgatgaagt aaagaaacac acaaatcttc cagtatgtgg ggttggaaaa ttgtctagcc
     1381 cagattttat agaaagtatt atttcaaata atagagtaga tatggtttct atgtcaagac
     1441 aattactagc agatagtaat tggttacaga aagtaaaaga tggaagagta gatgagatta
     1501 aaaagtgctg ttattgtaat aagaagtgtg cagatgcttt acaaacacgc tctcagtttg
     1561 gatgtatact agattagaat atccttataa aaataaataa tttgttaagg ttaatatatt
     1621 tttggtgatt tagataaata tttagataaa gaggaggaga catcatgaaa agtttagttt
     1681 tatattcaag tttaactgga aatactaaaa agattgctta tactatatat gatgaaattc
     1741 aagaagaaaa agacattaaa gatgtaaatg aattagttga ttatggtatt gactatgaaa
     1801 attatgatat agtattctta ggctattggg tggacaaggg aatttgtgat aaaaattcaa
     1861 aacaagtgct tgaaaatatt cacaataaaa agatagcact atttggaaca atgggagcaa
     1921 gtaaaaaagg aagttatgga gctagtataa tagaaaagat agaatcaatt atcccaaaag
     1981 ataatgaaat actaggttca ttcatatgtc aaggtaagat agcagaagga ttgaaagcaa
     2041 aatataaaga gatgttaaaa ctttctcctg acaacgaaca tattcgccaa caattaaata
     2101 accatgtgga atctcaaagt catccagatg aacaagaaat atatgaagcc agtatgtttg
     2161 caaaaaatat gatgattaaa gctagtattg tgtagtagtt aaacaataag ttcatataag
     2221 atatagaata aataatttag taaaaaagta tagcaaataa tttgctatgc tttttttatg
     2281 aaatcaaaaa aataataaaa atacttgact gtaaaccttg tttatacatt attttataga
     2341 aaataagata gttttaaata taaaaaaaga tatattattg aaaggagata ttattgtatg
     2401 aaaacttata aaagataatt agtttgattt taagatatac taaatataca attttataga
     2461 gtgaaaatat ttaagactat attatagtta taaattaata gaatagagag gtagtgttta
     2521 aattgcaata acagtatttc aaaagttgcc acctcaaatt cctatccaat ggaataatgg
     2581 tgtagctaat tatgtagata aaaaatttat ttatgcctac cctgtcattt gtgttgtatt
     2641 cagaatactt ttacgtccat ttatatggag atggttacaa atacatgctt tttatagtga
     2701 tactattgca gattatttaa cgaattttat gtgttttgta gcgttgtcta tagaaatatt
     2761 ctctatattt tttatttaca atattgtcag acatgttaca ataattattt ttatagatat
     2821 acttgtattt attgctttac tgataatagg tgtgtataaa ttatttaaaa aatacaatta
     2881 atttttaaaa ataagtaata agtgatagtg taaaaagtag ccactgcaaa ttatggattg
     2941 tagtggctat tttattattt gtatatgaaa ttattttgtt tttctaataa gttacgatat
     3001 attatggttt atttcactga taaaaggttt aggtatttta aagtaaaaag ttgtatattc
     3061 taataaaatt taaacatttt gattctttaa aattagagaa aatacattgc ttttattaaa
     3121 aaattaaata ttaagaattt aatttctgca tcttatttag aaaagataag aaatcaactt
     3181 tatatatttt ttttggcctt cctctttgat tatcgagttt tgtatttaca atagttgcaa
     3241 ttcctgcttt atataagata ctcaatattc tattagcact tctaagagtt atgttcatat
     3301 agtctgctaa tatttctgct gacacattat ttgaatttct atcattaatc atacacatga
     3361 ttttagatac atttgtatta gatagaggta taaaagaagc aactttattg gctattgagc
     3421 tatcttcaat attattagaa tttttatttg aatataaagg gcctattgta tcattagtat
     3481 catttactat aaaagtacag tttccattca aagaagcaga tttaccatta gcttgacgag
     3541 cattttgctc tgcatgaata tttgtatttc caatgcccca acctatattt aagctatatt
     3601 taatttcttt taaatcttct gaaataaaaa aatctgtata gttattggtc atattgttta
     3661 acatttcttt agtagtgtgt atctctattt tcatatcatt ttttttgacc tgaactccat
     3721 agaatccatg tcttgataga gaatttaata tagtgttata tatttgattt tgtatctcat
     3781 catttttaaa tatcaaatct tcatttatgt actctgaact atttactgta actatacaag
     3841 ttgcagattt atttttgtct aagttttgta atgttatttc attcaccaga gatttaaatg
     3901 tattcaggat tgattctgta gatggaacca tgtaaataga atcatatcca agtttattaa
     3961 gttcacaact aatgatgtaa tttctagtaa aagaaaggtt aatcttatta tctcgcaata
     4021 agtctaaatg tatttccaat aatttatcat ttaatttttt aaagtcatat acaggttgag
     4081 ggatattaaa atctttaatt gtatatggac atttattaga gtctattaat gaatatatat
     4141 cataataatt gttgtcctct cttaaaaaat ccataaatga ttttgtaaag tcaatatctt
     4201 tttggctatt taataatttg aataagtatt tataaaaatc tttttcatca agctttaaaa
     4261 aatttaatgg aacttttatg tcttttatat ttattataag ttcactatat ggaactatac
     4321 cactgcatac aaatccatca aaattatcta cttgctcata gtatatatta ggtaaattat
     4381 taaaaggatt ctccccttta agctcaataa atgtaatttc acaagtatca tttaattctt
     4441 taagagcttg tttaaactta tctgacataa aataagatgt tgtcaaaaaa ccaattttat
     4501 atttcataaa acctccttta aaatttcgag agtattgtaa ctaatcaatc aatttataac
     4561 ctcattttat aatagtagat aaaacgcgtc aatttatttt acaaaaggaa aattgaaaga
     4621 aaaatcagaa aatataaaaa agactgaaaa atatgttgac tgtcaagaat aattgtgcta
     4681 ttttgtaatt aaggactagt cttttaagat ttcctaaaat aaacttagat tttaggaaag
     4741 aaatacattt tatttgaaaa taaaggagaa gattatgaat acatatttaa atgaaatttc
     4801 taagtttgtt gatgagaata gagaagaaat agtttcatta tggaaagaga ttgttaatat
     4861 ggaaagctat acacattgta aagaaagcgt aaataaattg gctaagagat taaagtttga
     4921 gtttgaaaaa gaaggtttag attgcgagtt agtagatgtt ggagataatg gtagtacatt
     4981 gataggtaca cttggttcca atatagataa aaagccaata attttttcag gtcacatgga
     5041 tactgtcttt gaaacaggta catttggaga gaatccattt aaaataatag aaggaaaagc
     5101 atatggccca ggtgttttag atatgaaggg tggtataata atttccttat atgctattaa
     5161 agcattaaat aaaataggat acaaagaaag accaataaaa atagtatttt ctggagatga
     5221 agagatagga cataaggatt caacaggtgc agatgttata ttgagggaag caaaaggtgg
     5281 tctttgtgct tttaatatgg aaactggatt agtagataat agtttgtgtg taggtagaaa
     5341 aggaagaata ggatgtaata tacatgtaaa aggtgtagaa acacacgcag gtaatgattt
     5401 tgaaggtgga agaaatgcta tagaagaaat ggctaataag atactacgca tacaaaaatt
     5461 gactaattta gatgttggaa ctacagtaag tgtaagtgtg attaagggtg gaagagtgga
     5521 aaattctatt ccagaagatt gtagtataaa agtagacctt agatttgaaa aagtagaaga
     5581 aatggagaat gttaaagaac aagttagaga gatatgtaaa gaaacttaca tagaaggaac
     5641 ttctacagaa gttaatttta taagtgaaat gatgccattt gaaactacag aagatgttat
     5701 gagatttcat acatttgtaa atgaagtgtc aaaagaaaat ggatttggag aattaaatgc
     5761 taagagactt ggaggtagtt cagatgcaag ttatttaact attgcaaacg ttcctacaat
     5821 ctgttcattt ggtgtaagag gagaatggaa ccacacatca agagaatatg cagtagtaga
     5881 ttctatgttt gagagggtga aattaatttc tacagttatt ttaaacttag ataaatttga
     5941 ggtataaagg gggattttat atggatgtaa cagctggaga taataaggta acatggaaag
     6001 ggtatttatc tttatttgta ttgatagtat tattttcagg agtgtttaaa gatagtcaag
     6061 gcttccttaa agcattagat tttgctaatc tgacaggttc atttggtact attgcagatg
     6121 gtgtagactt tatgggtaaa ggaggaacgg gagcaaaaga tggtttctta tttgctctga
     6181 ctttgattcc aactgtctgt tttgctgtag gtcttataga tgtagttgaa tctatgggag
     6241 gaatgaaagc ggcatcaaag atatttaatc cattgcttag acctctgctt ggtatacctg
     6301 gaatagcagg aatagcattt gtatcttcat ttactagttc agatgtagcc tcaataatga
     6361 cgaaagaatt atttgaaagt ggggaaataa cagatgatga aagaactata tttgtagcat
     6421 atcaatatgc tggttcagct gttatactga acacaataaa tactcaagct ccacttttac
     6481 caatagcatt attggcatta ggtccaataa tacttataga gatattctgt aaaatacttg
     6541 gtgctaatct agttagatta atcataggtg taagaaataa aaagaataag gctaaggagg
     6601 ctgtgtagta tgagtcaaca aaatactgga aatgtaattg taaatagtaa acctaagaaa
     6661 actgctgttg agatatttat ggggggagca aaaaaaggtt tttacatagg tgttgaacag
     6721 atattaccag ccatgataat gggttatgta atagtgcagt ttttacaatt aactggttta
     6781 gttgatattt taggaaatat ttttggacca gtaatgggta tttttggact acctggggaa
     6841 tctgttgtag ttttgatagc agcattcttt gctaaggcag caggagcagc tacagctgca
     6901 aacttatttg cacaaggtct aatcactgca ccacaagcaa ccatattaat tatgccatgt
     6961 atgttaatgg gtactctagt tggtcattat gccagaatag tgcttgtagc aaatgccagt
     7021 actaagtata gaatgttact gttggcagtt cctatatttg atgcaatagt ggggatgttg
     7081 atagtaagag ttttactcac tgttatggga ttaatgtaaa taacataaaa ttataaccta
     7141 actataaatg atatataaga attaatatat acaaggatta atgagtagta attgatatat
     7201 agtaataaac taagatagta attaatgtgt atattaatta aaaaaagaga gtatctcaaa
     7261 gtgaactttt tagttcatga gatactcttt ttgtatgaaa tcaagtatat tttcataatt
     7321 tcaacaatga aagagaggct tatctctatt ttgagacagc ctcttctttt aaatttatat
     7381 ttctagaatt tatgtttttt atttatccaa cattaacatt agttattagc aataatttta
     7441 agaaaaatat actttcatgt taatatcaga ataaatgtgt ttttaaagga tgatttgttt
     7501 tgaattttca cgaatatcta cttctatgac tttttctatt gtatctcctc taaaacctaa
     7561 taaattactt gtcaagtttg cagtggaaca agaatgatta ggtataattc ttattctatc
     7621 accaattttt atatcagttt caccatttac tttaattttt ccaacttctt ctgaaagtga
     7681 atcaatagta agttctggat gtccacatac cctaccataa ccaacaatac tactatttcc
     7741 atgtgcacct ttatcaagtc caaggcactt acttccacaa tcaattaaat agtatccact
     7801 gctatttttt gagattacag tgcacacaac agtaagagca cacatatctt cagtagcaca
     7861 acctaaacct atctgtatag catcatagta tacatagttt ccaggtctta cagcagttat
     7921 agttttagaa gcagcttcag attcaactgt tggagtacta ccagaagcga caatttctat
     7981 attaaatcca ttatcagtta gagctttgta tgctttctcc atagaagaat cttcttgaac
     8041 acaaacatct ggaacacctt ctggagaact acaaccatac acttgacctg gatgcgaact
     8101 tataccaaca atcttgatgt tttctagctt agaaatttca gtagcaagtt caaccacttt
     8161 ttctggttca accccaaaac ggtgtaagcc acaatctaca ataataagta gcttgcactc
     8221 tatttttgat ttagaaaaag cttcactata agccttagca cattctatat tatctgtagc
     8281 acagattaaa tttatttttg atgccatagt cacaattcta tttatattta cttctcccat
     8341 gtaaggataa gccaacataa tatcttcaaa acctgctttt atcagagctt caccttcatc
     8401 aagtgttcca actagaaaac cactagcacc tgcatttttt tgaagtctcg ctatgtaagt
     8461 acttttatgt gtcttaagca taggccaaag ttgtttatta ttatttgtac aaatttgttg
     8521 aattttatct atattctttt ctaactggtc taaatctagc aaaaaatttg gagtagcgac
     8581 ttctgaaatt ttcatgttat ttaccacctt tcaacaaaag taaattaact ataagtatta
     8641 taattattat attagtaata tcggctttat aatattaata gcttataatt atattataga
     8701 actagcgtta acagagagca atatttattt gtaataatta gaattttcaa taagttagtg
     8761 tagttttaaa ttatttatat aatatatttt taattttata caaagtttat ttaaattagt
     8821 ttgaggattt attaataggg tattattgaa tgagtaaaaa agtgatgttt acatagaata
     8881 atcacactat aaattaattt agaacctaaa aaattaattg agcaaattgg aaaagagaag
     8941 gataaaaaag atatttggat agttggtgga ggcgatatag taaaattatt tatgaaagat
     9001 aacttaattg atgattatta tatatatata ttgacaataa tattggggag agggatttca
     9061 ctatttaagt ctggtataga taagactaat ttgaatttta agaaagctag taatattgga
     9121 gaacttgtaa aattggaata cagtaaaaaa caaaaataaa ttacatgcca atttgttaca
     9181 atgttatgaa acgaagcatt taaaagactt ttggctatat gaattgacat tacttgtcaa
     9241 atatgttaac atataagcga cttgtcggag ggtgtattaa aaagaatatg ctggagaaga
     9301 gagtgcgtta actcttttct ccttttttta taaaattaag catttataaa tttagtatta
     9361 ttacatgatt acactatctc aaaaattaag aaaggagaaa aatatgaaat taattttaag
     9421 ttatttaaag aattataagt tattaatagt attaaacata ctggctatat ttagttttgc
     9481 attagtcgag ttggggattc caactataat agctaaaata attgacaatg gtattgcaaa
     9541 tcagaatata gcttacataa aacaaatggg aatagtgatt gttattattt ctattatagg
     9601 ggttgtaggt agtatattgt tgggttattg ttctgctaag gtatcaacaa gtgtaaccag
     9661 agatataaga aatgacatat ttgagaaatc acaagaattt tcacatactg aatacaataa
     9721 atttggaatt tcttcaatga ttacaagaac aacgaatgat gcatttcaaa ttcaacaatt
     9781 tgttaatata ctactaagga ctgccttatt aactccagtt atgttcatta taagtataat
     9841 aatgacaatt agaacaagtg tggaactttc tatagtattg gctatatcag taccttttat
     9901 aattattgga gtagcaataa tagcaaaagt atctcaggca atatcttcaa aacaacaaaa
     9961 aggactagat aaattaaacc ttatatcgag agaaaattta actggaataa gagtaattag
    10021 agcttttgga aatgatgatt atgaaacaga gcgatttgaa aaaacaaata catattatgc
    10081 taatgtttct aagaaactat ttaagcttat gtcaattact caaccagcat tctttttact
    10141 attaaatata gctgttttgg ctgtattttg gatttcaagt gagaagataa atattggaga
    10201 gcttcaagtt ggacagttgg ttgcattttt ggaatatctt ttccatgcca tgttttcaat
    10261 tatgcttttt tcaatggtat ttattatgta tccaagagca gaggtatctg caaatagaat
    10321 aaaagaacta ttaaatgaag aaccattgat aaaaaatcct gaaaatggaa ttaaggatac
    10381 tgaaaataag ggtatcatag aatttgataa tgtaactttt acctatccag atggagaagc
    10441 ttctgtactt aaggatattt catttactgc aaaaacaggt gaaacagtcg cttttatagg
    10501 aagtactggt agtgggaaaa gtactctaat taacttgata cctagatttt atgatgtaac
    10561 agaaggtagc attaagataa atggagtaga tataagagaa tatgatttaa aagctttaag
    10621 aaaaaagata ggattcatac ctcaaaaatc actgcttttt actggtagca tagcaaataa
    10681 tattagattt gggaaacata aggcaggaga gtcagaatta gaatattccg caaaagttgc
    10741 acaagcatat gagtttatat caaaaaaacc tagaaagttt gatgaattga ttagtgaagg
    10801 tggagcaaat gtatcaggag gtcaaaaaca aagactttct attgcaaggg ctattataag
    10861 aagacctgaa atatacattt ttgatgatag tttttctgca cttgacttta aaactgatgc
    10921 aatacttcgt gctaaattga agaaggaaac aaaagatgca attgtattga ttgttgccca
    10981 gagaattagt tctattattg atgctgataa gattatagta ctcaatgaag gacaagttgt
    11041 aggaatggga actcataaag aattattaaa aaactgtgaa atatactatg aaattgcaac
    11101 atctcagtta aaaaaggagg aattagaata atgaaaaagt ctaaaggttt taagaaaaca
    11161 attggtagac ttcttccatt tgtaaagaaa tataaatttt cctttattat agctattata
    11221 tgtataattt cagcagctac tatgaatgca cttgcaccta aaactgaagg attgataata
    11281 acacaattga ctaaagatgt aattagtatc gcaaaaggag taccaggggc ttccgtaaat
    11341 tttgattatg taacaaaaat attggtgatt ttagcatgta tatattttgc caatgcaatt
    11401 tttacgtata cttcaagttt tctacttacg aatgctattc aaaacacaat gagagattta
    11461 agaaatgaag tcgaaaataa aataagaaga ttgcctatta gttattttga tagtaatagt
    11521 tttggagatg tattaagtcg tatatcaaat gatgtagata caatatcaaa tgcattgcag
    11581 cagagtttta tgcaaattgt aaactcaatc ttagtcataa ttttggcact ttcaatgatg
    11641 tttacaatta atatatatat ggctcttata gctcttttta taattccaat tagttatttt
    11701 gtatctaaat ttgtagtaaa aaaatctcaa agtagatttt cattgcaaca aaatgctctt
    11761 ggaaaattaa atggaaaagt acaagaaatg tatactggat ttaatgaaat aaaactatat
    11821 ggtaaagaag aagattctat aaaagaattt aaaaaagtaa atcaagaact ttgtgaaaat
    11881 ggatttaagg cacaatttat atcaagtatg atgaatccta tggtttcatt agtaacttac
    11941 tttggaattg cagctgtggc agttgtcggt tctatatatg cagtaagtgg aggaataaca
    12001 gtaggaaatt tacaagcatt tgtgcgttat atatggcaga taaatcagcc actttcacaa
    12061 atgactcagt tatctacagt aatacaatct tcttttgcag ctatagaacg tgtatttgaa
    12121 attttagacg aagaagagga aatacctgat gttgaaaatc cagttaagat agaaaatgta
    12181 aaaggaaatg ttacatttga gcatgttaat tttggatatg gagaaaattc aactttaata
    12241 gaagatttaa atgcagaagt aaaaagtggt cagatggtag ctattgttgg acctactgga
    12301 gcaggtaaaa caacattaat aaacttattg atgaggtttt atgatgtaaa gaaaggtgct
    12361 attaaaattg atggagtaga tataagagat atgaaacgta aagatttgcg ttcaatgttt
    12421 ggtatggtgc ttcaagatac atggttattt aatggaacca tttttgaaaa tatagaatat
    12481 ggtagatttg gggctactaa agaagaaatt atacaagcag caaaagtagc aaatgttcat
    12541 cactttataa ccactcttcc agatggatac aatatgtttt taaatgaaga agcatctaat
    12601 atatctctag gggaaaaaca gcttttaacc atagctagag cttttatatc agacccttca
    12661 atattgatac ttgatgaagc tacgagttct gttgatacta ggctggaact tatgcttcaa
    12721 aaagcaatga gaaatcttat gaatggtaga actagttttg ttatagcaca tagactttct
    12781 acaattcgca atgctgactt gattttagtt atgaataatg gaagtattat agagcagggg
    12841 aatcatgatg aattaatgga aaagggtgga ttttatgaga agctttataa tagccaattt
    12901 gcagacaaag aatcagaata gataacataa acttttgtat aaaattttgt atcaaattca
    12961 aatattaatc atataattaa aaagagtatc agtaagttgt ttttttatag atttactgat
    13021 actctttgat tttaagacta aaggtactta tgattggtac ttataattca caactagtct
    13081 tgttacatct gataaccaga attacagtac ttttcttttt tacctttttg ttctctaaca
    13141 tataacaatt cgcttaactt atcaatgtaa taaccatcat cacttatgcg aattaaacta
    13201 gtcagataat atccatcttt gcttctagga atctttttat ttattgctaa atcaatagct
    13261 gtctttgctg cttcaaaatg ctttaaatgt gtatgacctt cttcaaaact ctttctagta
    13321 ttataaacta cataaccttt tttaacttga aaaactataa attctttctt ttcatatatt
    13381 ttattaaaca ttcaatcagc ccttctcaag atagttttat agcataacat tagaagcttc
    13441 taaaaaattt tacaaagaac ataaatattt tataaattat aaatctaaaa ctataattta
    13501 taaagataac tttctgatta tttatatgat aattcatata gattaaaagt aaattatctt
    13561 taggttaaac aaaagtaaaa taaaagacta gttataatat ttatatgaaa aagtacaaaa
    13621 tatagtatat gaatatagaa aatttaaatc atttatttac agtttttatt tccataagta
    13681 tatttacctt cagatacctt gtcatcatgg aaatatattc tttcacaact tttatctccc
    13741 catagaatgt tttttgttat tttatgggac gtatctcctg taaatacttc agctacagta
    13801 gtatctgtaa catcacagta aagagaagca aattttttaa accaaggatt ttcctcaaat
    13861 cttaaaagcc aagaagctcc ataggggcaa tgtaactctt caaattcagg aacccatcct
    13921 agaaatggaa catcagttgt gctaataata ttgtctgcga ttaattcatc tccattttca
    13981 tgagctctct ctctcatagc aatagctctt tcttcagacc tttcaaataa aacttttctt
    14041 aatatttcgc tagttttatc ttcaccaaac tcttcaaata acactttgga aaaataaaaa
    14101 tataaatcac caaaatgttt gcatgccata cgaacttctt caatagcata atctttgtca
    14161 atagtttcat tagttacaac atcttcgttc aaaaaaatca ctccttagaa taagtattag
    14221 ttacaatata attatagaat attttttata aattaggaaa ttaatttgag atagatatat
    14281 ttttaataaa tgaaagtata atactaaaat aacttattgt tacaaaaatc ttctaaatta
    14341 atgtatttag gagtttccat atgaatgaga gtagttctgt aaatatcttc aatacatcca
    14401 tttgcaatat tattaaaaat atctgtgtgc tcttttaatg ttgtttcaag ccttccttca
    14461 tgtgacaatg aatcgagagc taattttttt attctgtaca tataattgtt aaacattttg
    14521 tcaaattcag tattttgagc atatgaaact atagttgtgt gaaataatgt atcgtattct
    14581 gcaaatgagt ttaaattttt atcacctaat aaaatatctt tttgtttatc taaaagatgt
    14641 tttaatttac ttatagtttc tactgctcta gcttctttgt attgacttgt aattaaaaaa
    14701 gtacaaaatc cttctattgc agagcgtatt tggaatatct ctacaatttc atttgctgta
    14761 atttgatgga ttctaaatcc tttgcttggg attatatcta taaatccatc ttgactaagt
    14821 ctttgaagag catctcttat aggtgttcta gagataccta tttcactagc aagttttgtt
    14881 tcagaatata tttcattttc tatcatttct ccagatagta tcaattcttt taagtagtca
    14941 tatgcttgtt cttgtaatct tttaaatgta gccatactta ataatacccc cctaaacaat
    15001 ataaaaatac aaaaacttat ataattttaa gcatacaaca tagtgaaaat aaggtcaatc
    15061 ttttgcaatt taaattgcag ataagattaa aaaatataaa aaattattga agtgtgaagc
    15121 atgattaaca ttaaaggcag attaattaaa actaattgaa attagttaaa atacaaaact
    15181 gtaaaagtag tatattatat ttgaaaacaa tagtatgtat taaaacttat ataggatgac
    15241 taaatataat aaatgacttt aaaaattact atttattgtt aaaataaata cttttatatt
    15301 ttatattgac cggtatactg gtatgtagta tactctaaaa cataaaaaac gttttcaaaa
    15361 atgtgaagat atggagggtt ataaaatgaa attgggattt attggattag ggataatggg
    15421 aaagccaatg gcaaaaaatt tattaaaaga tggatgtaat cttttggtgt atgacattaa
    15481 taaatctgct gtggatgaac ttatatcatg tggagcaaaa tacgcaagtg ttttagaaat
    15541 gggtcaagaa tgtgatattg tatttacaat ccttccaaat ggaactattg tgcaggatat
    15601 attgtttggg atggatggat tggcaaaaac tttaaaagaa ggaagtatag tagtcgatat
    15661 gagttcagtt actcctacag aatcaatatt gtgtgcaaat aaattaaaag atatgggctt
    15721 agaatttata gactcaccag taagtggagg ggagccaaaa gcgattgatg gtacacttgc
    15781 ttttatggct ggtggtaagg aagaaattta taagaaagta gagccatttt ttaatataat
    15841 gggaagctca tctattttaa ttggagataa tggtagtgga agtgttacaa aacttacaaa
    15901 ccaagtaata gttaatttga cgatagcagc agtttctgag gcatttgtac ttgcagcaaa
    15961 agcaggtgca gacccagaaa aagtatataa ggcaataaga ggaggcctag ctggtagtac
    16021 aatattggat gtgaagatac caatgattat gaatcgtgat tttaaaccag gtggaaaaat
    16081 atctattaat ttgaaagata ttaagaatgt aatgcaaaca gcacacaatt tagatgtacc
    16141 actacctatg acaagtcaat tattagaaat tatgcagaca ttaaaagtac atggacattt
    16201 ggaagatgac catagtggaa tagcacagta ttttgaaaag ttggcaggtg ttgaaataaa
    16261 aaaacacgat aattaatatt taaatatatc aatataatta aatacatatt attaggatta
    16321 gattatataa atacagcgag gttaagttat catagtaaaa gaaaaataaa attgataaat
    16381 aataaagtag aatgataaag gggtaaagat tatgattaca ggatatggac ttttgatagc
    16441 atttgttata tctataggaa ttttacttgt atcgattatt aaatttaaag taaatccgtt
    16501 tttggcatta ctgataacat ctataattac aggctttatg gttagaatgc ctataaatga
    16561 aatatcaacc acaatatcga ctggatttgg aaatacatta ggtagtatag gtattgtaat
    16621 tggacttgga ataatatttg gaaatattct ttcagaatca agagcaacag aatctattgc
    16681 aaaggggttg ttagctaaga ccggagaaaa aaacagtgct ttagcagtaa ctacagcagg
    16741 ttttttaatt tcaattccag tttttatgga tgcagctttt gttataatga tgcctataat
    16801 taagtatgtt tcgagagtaa ctaagaaatc tctaatggtt tttgtatgtg ccttaggagt
    16861 tgggactata gtaggtcatg cacttgtaat acctacacca ggacctttgg cagttgcagc
    16921 aaatgtaaat gcaaatgtag gtagttttat actttattct ataattgttg cttttccagc
    16981 agctcttata ggtggatgga tttatggaaa acgttttgaa aaatatccag cttatgctat
    17041 tgatgagaat gatagagaaa aaaatttaga gcagggaaaa gatagcataa aaattgaaga
    17101 tgatgattct tctaaagtac caggttttgg aatttctatg ttttctttat tgttcccaat
    17161 acttcttata cttatatcaa atgtatttag tatgttctta gaaaaaggtt ctacaatgag
    17221 tggagtactt gcatttatag gagataaaaa catagcaata cttcttggaa tattggtggc
    17281 aataggattt ttaaagaaat acataaataa acctatggga gatgtagtta ttgaagctgc
    17341 tgattcagca gggctaatat tattgataac agggtcaggt ggtgcgtttg gcagtgttat
    17401 aaatgcaagt ggtataggta acttcttagt tgatactatg tctggtctta gtatatcagt
    17461 tgtagtactt ggattcttat taagtgcctt gcttagaatt tctcaaggtt ctgctactgt
    17521 agccttagta acaacatcat caattttagg acctacaatt ttggcaactg gaatgtcacc
    17581 agttttagta gggcttgcta tatgtgctgg aggagttggt ttctcccttc caaatgattc
    17641 tggattctgg gtactttcaa gatttagtgg attatcagtt aaagatactt taaactcttg
    17701 gacaattgga ggaaccattg ctggagttgt agcatttata atggttttat tgcttagtgt
    17761 tataaatgga attataccac ttccaggtct ataaataaca aactattgtt tatatcatat
    17821 aaattatatt tagactatac aaattacata aaggggcgtt ttaaatgaga gaagtaaaac
    17881 caattagcat agatatactt aatactttta aacaagtaga tgaggataga ttaaataaac
    17941 ttcttgcaga tgagcttaag catttagata gaaaaattgt tgtattagat gatgacccaa
    18001 caggtgtaca aacagttcat gatatttctg tttatactga ttgggataag gatagtatgg
    18061 aacaaggctt taatgagaaa aatagtatgt tttttatact taccaattca aggggattta
    18121 ctgttgctca aactacaaaa gcacataaag aaatttcaaa gaatattgtg gatgtatcaa
    18181 agaaagttaa taaggatttt ataatcataa gtcgtagtga ttctactatg agaggacatt
    18241 atcctcttga aactaattta ttaaaaagcg aagtagagag attatcgggg aagttatttc
    18301 agggtgaaat aataatgcca ttttttaaag aaggtggtag gtttactata gataatgtac
    18361 attatgttaa agaaggagaa atacttgtac cagcaggaat gactgaattt gcaaaagata
    18421 aatcttttgg atataaatca tctgatatag gtgagtggtg tgaagaaaag acaaatggaa
    18481 agtacaaatc tagtgatatg atatatattt cgtcaaaaga acttagagag ctaaatattg
    18541 aagctataac agaaaaactt aaaaaagctg aaggattcaa taaaattatt gtaaatgcaa
    18601 ccgattatgt agatgtaaag gtatttacaa ttgcgtttat acgagcagta aatggtggta
    18661 aggaatttat gtttagaagc gctgctgcca taacaaaagt acttggcgga gtgagtgaca
    18721 aggaactact tactaaggat gaattagtat caaaaggtaa taccaatgga ggaattatac
    18781 ttgttggttc acatgtgaat aagactaccc aacagttaga agaacttaaa aattgtaaat
    18841 atccgataga atttatagaa tttaatcaac atttagttct tcaagaaaat ggtctaaaaa
    18901 atgaggtaaa aagagttata aaagttgtag aggaaaaaat aagcagtggt aaaactgttg
    18961 ctgtttatac tagacgagag cgttttgact tggatacgaa tgataaagat aaacagctta
    19021 tgatttctgt agaaatatca gatgcagtta ccagtataat tggtatgctt aatgtaagac
    19081 caaattttat tatagctaag ggtggaatta cttctagtga tgttggtact aaagcactaa
    19141 gagtaaaaaa agctacagta atgggacaga taaaacctgg tataccagta tggatgacag
    19201 gcaaagaaag caaatttcct aatatgccat atataatttt ccctggaaat gtgggagaga
    19261 ttagcacact tagagaatca gttgagatat tgatggggta gtatattagt gtattgaagt
    19321 ttaaacgctt aaatttactg agttaaaatt aaaataaact aaaattataa attaagatta
    19381 aaaataatag tatattaatt agggtttaga gtattttaca aaaaatattc taaaccttaa
    19441 tcttatgtat tagtattttt attatttaac ttatgtgttt attttattat ttatcttttt
    19501 taattgattt aatattttct ttgattttat ttaaaatatc tgtatgctta ttagagctta
    19561 tgttggggaa tgctttgatt atacgtctat gaagtaattt gtggttagac agcaaagtat
    19621 taagtttatt ttttagctca tcaatacgat ttttagcata gcgataattt tctttttctt
    19681 taagtatttc aaacctatct tccaaagtat ccagtctatt ttcaactaaa gattcaagtt
    19741 tgttttctat agtttcttgt gtaataagtt tgtagtattc tttcttatct tttaattcct
    19801 taattataag attgatttgt tttaacttac catttaattg taaaattgta tggactgtga
    19861 taactaaatc taaaacaaaa taaatttcta tagatatagc aaatataaat agcacatttt
    19921 tagagataga gttaataaag tcaaccacaa taggatgtac tataagcatt acaatcaatg
    19981 ataatacacc aaacataagt gagttttcca gacaaactct tccatgaatg ttaaatttat
    20041 gtttagaata atcccaccaa gtcatattaa atattttttc taaaataaag ctcgtacagt
    20101 actccattat gctagttata attatactaa acaaaaataa caaaggaata ttgtgtacaa
    20161 aaggagtcaa ggtagcaact attgctaatg ctccaaatcc atatataggg caaaatggac
    20221 catttaaaaa acctctattt acaaattgtt tttgtaaaac tgaacaatat gtagtttcac
    20281 aacaccatcc tataaagcta taaattacaa aataaagaaa ttgaatataa aatgtactca
    20341 taagcacctc atttctagtt agtataacac tatatactcg atattttatt tttatgatta
    20401 atttactaaa ttcaatttac atattgatta taccaattta tagaaattta tcaaatttaa
    20461 ttacagttag aacattataa acatggttat aataatgaaa ttttatttaa aataaataag
    20521 ggtgtcaagt ataatcaaac taatagttgt agatactcta cactatgtta gtttaattac
    20581 atttgacacc cttatattta tactatgact atttacattc gaaactagag caacatgttt
    20641 caatataaag gtttactcta tcagtttcag gagcaaccaa tatattatca agtgtacata
    20701 agctaccatc tttgttgtat ttacaatttt ttacagtaca aactaattcc tcacaatttt
    20761 tattattaac gttgctagat aaattactat aaatagattc atctaaaaat gaactacaac
    20821 aagtatgtct gtcattgtga gctttttcac catttataaa aattttatct gcataacata
    20881 tatggctgtt gttatgtgaa cagttaatta cattacagtt tattctttcc aaagttatta
    20941 cctcctaaac gattatttat tataaattat gtccatataa ttacaagaat ataaccatta
    21001 agagaaaact accttttaga aaatatctat tcttagagta atttgaaata taaatatatt
    21061 ttcttctaaa atttacctaa attgtataaa aacttacagc ttggagaaaa taatagaaaa
    21121 aggaggcgta atcaatgttt gaaaagaaga aaaaagaatt atttccttgg aaaatatctg
    21181 ccttaatact ggttggagta tttgtattaa gtagcggagt ttatataggg acaagattaa
    21241 aacacaatgg agttcaaact ggtgaagttg taagtactag tgcgaatatg aatgaaaagt
    21301 caaaagaagc ttttggatta aataaaaatt gtgaagtttg gcttcaaagt gaaggcgaaa
    21361 atgaaactaa ctataagaga acacttacaa tggcaggaat agttccagag tcattattag
    21421 ataagacgaa gtcagaaata gtatcatatt ttaaaaaaga atatccaact aaaaaaataa
    21481 agagtatgga taaaaatgaa atcatattgg tagaatctaa atatagtgat aataaagatg
    21541 tacaagcaat ggatgttact ataaaaggta aatatactat tgaaaatgat aatggaaata
    21601 tagcactata taaatatgat gatagcggaa gtaaaacatt agtagaaaaa acaaaaataa
    21661 gagtagattc acttcctaaa acagttcaag atgaaatcaa aaaaggtgta gttatggata
    21721 ctgaagaaga ggcctattca agattagaag actttgcaag ttaatagtaa attaatatta
    21781 ttttaagtta aatactaagt aaattaataa taaataattt aatattaaat agattagtat
    21841 taaaattgta gtcacattta attcctataa aaatacaata atttatttgt aataaatata
    21901 aaagttatat tgaggtaaaa tcttgctttg atataacttt tttatttgat tttatatttt
    21961 acatagagca ttttatgatt tgttaatatt atttatgata taatgattta gaacataagt
    22021 ttttatgaga aatacatttt ggtatttctt ttatttttag ggggaatttt aaaaaatgat
    22081 aatattgggg attgacccag gtatagccat agttggatat ggtataattg aatacaaaaa
    22141 tagcaagttt aaagcaatcg attatggagc agttacaaca cctgcccata tgaatatatc
    22201 gagaagattg gaacttgtgt ataaaggaat tgatacaata gtaaagaatt acaatataga
    22261 tgaagttgga atggaagaat tattctttaa caagaatgta aaaacagcta taacagttgc
    22321 acaagctaga ggtgttacta tgcttgcatg tgctcataat gggaagcctg tatatgaata
    22381 cactccactt caagtaaaac aaggtgtagt tggatatggg agagcagata aggcacaagt
    22441 tcaacagatg gtaacttcat ttttaagtct aaaaaaagtt ccaaaaccag atgatgttgc
    22501 agatgcttta gctgtggcta tttgccatgc tcattcaaac aaacttgaaa aaactctaaa
    22561 gaatataggt ggtaagtatg tatagttata taaaaggtac agtagaagaa atttatatag
    22621 acagtatagt tgttgaaaat aatgggatag gatataaaat aaatgtatca tccaatacaa
    22681 taatgaattt acaagtggga gaagccacaa aaatatatac taaattaatt gtaagagaag
    22741 atgatatgag tctttgtggt tttgtaagta gggaagaact taagatgttt gaattactaa
    22801 catctgtatc taaaatagga cctaaggtgg cgctttcaat actatcattt gcatcaccag
    22861 cacagttagg agcatatatt ttaagtgaag acattggaaa attatccaaa gctcctggtg
    22921 ttggtaaaaa gacagcagaa agaatcgtct tagagcttaa ggataaggta gacaaaaata
    22981 acatagagtt tgaaccaacg ttattatcac aaaaacctac cttgatatca caagatgagt
    23041 ctgtagatgc acttgtagct ttaggctata ctttgtcgga atcaaaagag gcagttcaaa
    23101 aatgtaaaaa agatggtatg aatacagaag ctattataaa gaaagcactt acatatataa
    23161 tgagtaaatc cttgaaatag tatttggatt ttataatatt tggattttaa aacaatattt
    23221 aaggagttta tgtaaatttg aaaatatata aagtagattt tcaaaagtac gagaggaggg
    23281 gtataaatgc aaggttttga agatgaaaat aggataataa cttctacaat gaagatggaa
    23341 gatatagata tagaaaacag tttaagacca aaaactttag aagattattt gggtcaagaa
    23401 aaatccaaag aacaacttag tatatttata gaagcagcta aatcgagaaa tgagcagtta
    23461 gaccatgtgc ttttatatgg accaccaggt cttggaaaaa ctacactagc aagtataata
    23521 gccaatgaga tgggggtaaa tttaagaatt acgtcaggac ctgcaataga aagagctgga
    23581 gatttggcag ccatacttac gaatttaaat gaaaatgatg ttttatttat agatgagatt
    23641 catagaatca atagaagtgt tgaagaagtt ttatatccag caatggagga tttttgccta
    23701 gatataataa tagggaaagg gccatctgct agaagcataa gacttgacct tcctaaattt
    23761 acacttattg gagctactac aagagctggt atgcttacaa atccacttag agatagattt
    23821 ggtgttattt gtaaattgga ctattataca gttgatgaac tttcaaaaat agtacttagg
    23881 tcttcaagca tcttagatgc agagatacag tctaatggag cattagaact tgcaaaacgt
    23941 tcaagaggga ctccaaggat agcaaataga ttactaaaaa gagttagaga ttttgcacaa
    24001 gtcagagctg atggaaaaat aactgataaa gtggcaaaag atgctctgga acttttagga
    24061 gtcgatagtt tgggtctcga ttttgtagat gagaagcttc ttatgactat aatagaaaaa
    24121 tttagaggag gaccagtggg tttagataca ttggcagctt ctatcggaga agatagaaat
    24181 acaatagaag atgtatatga gccatatttg cttcaattag gatttataaa tagaggtcct
    24241 agaggaagag tagctatgcc attagcatat gaacacttaa aaattccata tcccaatgaa
    24301 aaataaaaat agattatacg agtatttata aaataaaaga gtcattaaat taaaatattt
    24361 ctaaaatggc taaaaattca aacatatcca caaaatggta attatatctg tagatactgt
    24421 ggataaacac gataaaaatt gtgtgtattg tatctatata ttgtggataa ggcatgtata
    24481 tcgtggaaaa atatctatat attgtggata tggtgtctgt taaaaatagc aatttcttga
    24541 tagattatgg taatattgtg gaaaataagt tgtttttttg atattattgt agctggagaa
    24601 attttaagag aggaagttta acattgaaaa caagtgattt taaatttgac ttaccacagg
    24661 aacttatagc tcaagttcct atagaagata gagctagctc aagattgatg gtattagata
    24721 aagagacagg taatattgaa cataaagtat ttagagatat aatagaatat ttaaatccag
    24781 gagattgctt agtattaaat aatactagag ttataccagc tagattaata ggagagaagc
    24841 ttgaaactgg aggtaaaata gagtttttat tattaaaaag aacagaggaa gatacttggc
    24901 aggctttagt aaaaccagga aaaagagcaa aagtagggac taaattttct tttggaaatg
    24961 gaaagctaat tggagaagtt gtagatttat cagatgaagg ttcaagaatt ataaaattcc
    25021 attatgatgg aatttttgaa gaaatattgg atgaacttgg aaatatgcca ctacctccat
    25081 atatcactgc tagactagat gaaaaagaaa gataccagac tgtttattca aagcataatg
    25141 gttcagcagc agcacctaca gcaggattac attttacaga agaattgtta aataaaataa
    25201 aagaaaaagg tgtagatata gcctttgtaa ctctacatgt tggcttagga acttttagac
    25261 cagtaaaagt agaggatgtt ttaaatcaca agatgcattc agaatattat atggttagcc
    25321 aagaagcagc tgacaaaata aatagagcaa aggaaaatgg aaaaaatgta atatgtgttg
    25381 gtactactag ttgtagaact atagaatcag cttgtaatga ggatggaaag atgaaagaaa
    25441 ctagtggatg gacagaaata tttatatatc caggttataa atttaaagtt ttagataaac
    25501 ttattactaa ttttcactta ccagagtcta cactaataat gttagtaagt gctatttgtg
    25561 gaaaggataa tgttttaaat gcatataatg aggcagtaaa agaaagatat agattcttca
    25621 gttttggaga tgctatgatt ataaagtagg gaggtaatag atgcatagtt tacaaaaaca
    25681 aaaaaagaat aaccctatat taaagattgc ttttatattt gaacgtgttt tagcagtagt
    25741 agttctaata gcagtatttt taggaactat agatgttcta agacttatgt ggggagcata
    25801 tatagttgat tttcaaaatc cagtacaata cagtcagctt aatgattttt tagcacaaat
    25861 attactttta gtaataggtg ttgaacttgt agtaatgcta tctcttcata taccaggagc
    25921 atttattgag gcattacttt acgcaattgc tagaaaaatg ttgctactgc ctaaaaatga
    25981 aggtatgata gatgttttaa taggagttat agctatagca ggactatttg ctataaaaaa
    26041 gtttttagta acaaaagaca agagtgctct taatataatg agtattcatg atgaagaagc
    26101 aattaaggaa caacagaaca aagaagacga agataaatct atttagacaa taaattatat
    26161 gaatagctta ttacaaatat atcttaagaa ataatgagaa ataaatatga ttaaggagaa
    26221 ctttatgtac gcagtaagat acgaattgat aaagacttgt aagcagagtg gtgcaaggtt
    26281 aggaagatta cacactccac atgggattat tgaaacacca atattcatgc cagttggaac
    26341 tcaagcaact gtaaaatcta tgacaccaga agaattaaaa gagattggtt cacaaataat
    26401 acttagtaac acataccatt tatatatgag accaggtcat gaacttataa aaagagctgg
    26461 tggtcttcat aagtttatga attgggataa accaatactt acagatagtg gagggttcca
    26521 ggtatttagt ctagggcctt taagaaagat aaaagaagaa ggtgtagaat ttaggtcaca
    26581 cttagatggt tcaaaacatt ttctaactcc agaaaaagct atggaaatac aaaatgcatt
    26641 gggttcagac ataatgatgg catttgatga gtgtgcacca tacccatcag atagagaata
    26701 tgtaaaaaat tctttagaaa gaacaacaag atggttaaaa agatgtaaag atgcccataa
    26761 taataccgac aagcaagctt tatttggtat aatacaaggt gggatgtata aagacttaag
    26821 ggaacaatca gcgaaagaaa taactaatat tgatttacca ggatatgcta ttggtggact
    26881 tagtgttgga gaaccaaaac cacttatgta tgacgtgtta gagcatacaa ctccacttat
    26941 gccaaaggat aaaccaagat acctaatggg agttggtagt ccagatgact tagtagaagg
    27001 tgttataaga ggagtagaca tgtttgattg tgtcctacct actcgtatcg ctagaaatgg
    27061 aactgcaatg actagccaag gtaaagtagt agttagaaat gcaacttatg ctgaagactt
    27121 tactccactt gaccctgaat gtgattgtta tgcttgtaaa aactattcaa gagcttatat
    27181 aagacacctt ataaaagcaa atgaaatact gggtgcaaga ttaataacta ctcataatct
    27241 tcacttttta ttaaatttaa tgaaacaaat aagacaagcg ataatggaag atagattact
    27301 tgattttaga aatgaatttt ttgcaaaata tggatatgaa atataggttt tgcactatta
    27361 attataatca ttttaatata taatattcaa tagtactcaa agatagagaa agtaaattgt
    27421 tatataaaca tttattatga tttacaaaag agtataattt acaaaggagt gaaaaattaa
    27481 tgccacagca acaaataatt atgagtatag gtctatgggt agtagtaatc gcaatctttt
    27541 atttcctaat gattagacct caaaagaaaa aagacaaaca attaaaagaa atgagaagta
    27601 gcctaagtgt aggagacaag gtcattacaa taggtggaat agtagctaat gtagctaaag
    27661 ttgaagatga tgttgtaata ttagaattag gaccaaatag aactaaagtt ccatttgaaa
    27721 aatgggctat aggaactgtt aaatctaaaa aagaagaaat agaagaagac taaattagta
    27781 atttaaaagc cttttctcga atatatatta atttgagagg aggttttttt atgggaaaat
    27841 ctaattatat ttttaaaggt ctaggatatg catatataat aactttggct gttttattag
    27901 tgtataactt atttttgaca tttacagata ttggtggaga taatataact atggtctcat
    27961 catttataac tactatttct gcagctatag gagggtttta cacttcaaaa cacatgaaag
    28021 aaaaaggact tatgtatggg cttttagtag gacttttgta catagtatgt atttttttga
    28081 ctgtgttttt agcacaagaa aagtttgtat ttgaagtagg gatgatttat aagttattgt
    28141 taatatcagc agcaggaggc attggaggcg tattgggtgt caactttaaa tagagtttat
    28201 attaaagtag ttttatgata taatcaatag gaaacaatta gaaatggagg cgttgaaaat
    28261 ggaaaataaa catgtaaaaa ctttatctca agctacttta aagcaaagtg ctgcaaaagg
    28321 tggatgtgga gagtgtcaaa cttcttgtca atcagcttgt aaaacttctt gtacagttgc
    28381 aaatcaagag tgtgaaagat agaaaattat tgatttagtt gtttgtaaga gggtatctca
    28441 aagtgattct tttatcatta tgaggtatcc tcttttattt tcaattatta tataaaaata
    28501 tatattctat ttatgtaaaa ttaaatttga tttcttatcg tttaattatg caaaaatatc
    28561 taatcatata agagcattta ttttaaagta gctactctta tattttattt tttatcaatt
    28621 acatgtatta gaataatttt taattttaaa ctctaattta tgatgatttt tataatatgt
    28681 tatatatttt aatatatgga gatagatatt aatttagtga tatatattta agtaataaga
    28741 gtaaagcaaa aattagtgca tagtagtata taaaaatgga gtttgaatac ttttgttagt
    28801 atatgattgt ttagtagaaa atcactataa aataggattt ctatctttga gcataattat
    28861 agagagctaa gcagggggaa ggggttcaag aagatattat tagtgtttgt gttagtaaat
    28921 tatgtaaaca cataaaatat attgaaaaat aatattatat ataatattat ttatgtgtgg
    28981 gaaatacgaa tataaaaaat aaaaaataat attcggagga cataaatatg ttaagcaaca
    29041 aaaagagaag tatggcaata gtaatggcag gtgctacagt tatgagtgca gcagcaccaa
    29101 tattcgcaga caatactgtg acagaaaatg ttgataaaaa ttacacagta agtgcgaaag
    29161 attctgctaa actaatcgaa gaggttagaa aagcattaga agttaagttt gaagatacaa
    29221 aagcaggagc aaatgtaaat gatagagttt acgatataaa agtagacaat gtaaacttaa
    29281 ctaatgcaac tcaattacaa aataagataa actctttaac agaagggcaa agtttaaaag
    29341 ttactattca agataaagga catcaagtgt taggtggaaa agtagttgac tataaaattg
    29401 aaaactacaa aactgctcaa gaaatagtag atgcagttaa tgcttacaat gcaactttag
    29461 cagaagattc tgataacaag ttaacagcaa ctataaaatc tacaaataca gttgaagtaa
    29521 aaagagcaaa agattcagca aatgttatta cattaaatgt aggagaccaa catttagatt
    29581 tctcaaaagt gataacatca gaagaaggta cttttgaagg ttatgaaaag agttatagtg
    29641 acatagattc aaaagaatta catacgataa cagttaaaaa cgcagattta caagacatat
    29701 cagcagaaga attatttgat ggaattagat taactacatt aggaagagaa atagttaata
    29761 aagttaaaaa tggatatgct ttaacatttg aaaatgaagc aatccttact caagaacaag
    29821 aagatagtga tgataaagat aaaccagaaa aatcaagctt tgatatagtt ttaagcaagg
    29881 caaatgaaaa gccagaaaca ataagtgttt caagtaaaaa ccataagtta gttagagact
    29941 tacacaaagt attaactgat gtaaaaggtg gaaaagaatt aaaagtagaa gttttatctg
    30001 gtgattcaag atttacaaca gcagtagaag ttagtaaaga gagattcaaa gatggagaag
    30061 cagaagctat aattttagtt ggagaagatg ctatagttga tggattagca tcagcaccac
    30121 ttgcctctca aaaaaatgca ccaatattat tatctaaaaa agattcacta ccatcagaaa
    30181 tagaagctga aatattaaga gtacttggaa gtaatctatc ttctaagaaa atatatatag
    30241 taggtggaga atctaaagta tcaaaagaaa ctgaagaaaa actttctaaa ttaggtgtaa
    30301 gtaaagttga gagagtttct ggagaagata gatttgaaac ttctttagaa atagcaaaac
    30361 aattaaaaga tacatttaag actgcttttg tagtaggtgg aaatggagaa gctgatgcta
    30421 tgagtatatc agctagagct gctcaatttg gtgctccaat aatagttaca ggtaacgaat
    30481 tagatgcaaa tgctgaaaaa ttattaaaag gaaaagaatt agaaatagtg ggtggagaaa
    30541 attctgtatc aaaagaagtt gaagacaaat tagttgatat agatttaaat aataaagttg
    30601 aaagattagc tggagaaaat agaaaagata ctaatgctaa agtaatcaat aaatactatg
    30661 caggtgcaac taaagcatat gtagcaaaag atggttatgt aggtggaaat ggacaattag
    30721 ttgatgcact tacagcagca ccacttgcag ctagttcaaa ggctccaata gtattaacta
    30781 cagaagaact ttctaaatca caagaagaag tagttgagtt aagacttaag aatgctacta
    30841 agttagtaca aataggtgaa ggaatagcta aaaacgctat tgaaaaaata gcagaaaaaa
    30901 taaacttatt tactaagaac taataagata taggaattaa tattaaaagt ttgtttagat
    30961 aatatatgat tatacaatgc gttaatatgt aagacatagt gtagatatta taaaatatac
    31021 aaataacaag ataaaataat aaaaataagg tgtctcattt agaattagat tgagatacct
    31081 tattttattt tttgattata atgttgagaa attcatattg caaaaattat tataaaaaga
    31141 tacttcttat aatgattaaa catcttatta acataaatta ttttgaactt ataacttaat
    31201 taaataaaat aaattataat gttttgaatt tatagagaat aaaaacttta ggtatattgt
    31261 ttgagttgta tggataacta tggtatcata tataaaatat ggtaacaaaa ttgtaacttt
    31321 tgattaaaaa taagtatgag tactaataaa aaatatgaaa agtacattgg gggtagaaaa
    31381 taatatgaag aatagtaaaa agatattagc tataggactt acactatttt tagtaatggt
    31441 aaatactcct atggtaaatg cgttgacatc agttgagcaa ataaaaggga atgacagata
    31501 tgagacagca gcaaaaatag cagataagca aaactacaat acagcaatac taatcaactc
    31561 agataatagc ttagcagatg gtctaagtgc aagtggttta gctggagcct taaatgcgcc
    31621 tattttgatg acaaaacaaa atcagattcc aaacacaact atggaaagat taaacaaagc
    31681 aaagactgtg tatataatag gctcagaatc aacaataagt aaaaatgttg agaatcagtt
    31741 actatccaaa aagaaggttg tacaaagaat ctttggtgaa aatagatttg atacaagtat
    31801 aaaaatagct gaaaaaatca aagaaattaa gccaatagat aaagtaatta tagccaatgg
    31861 atttacagga gaagcagatg cgataagtgc atcaccagta gctgctagag atggagtacc
    31921 tataatactt acagatggaa atagtgtggg atttgacacg acaggcttaa agagttatgc
    31981 gcttggttct agtgaaataa tcagcgacga attagttaag agcacaaatt ctattagatt
    32041 gggtggcaca gatagatttg aaactaataa gatagttata caagagtttt ataaaaatag
    32101 taaagaattt tatctaagta aaggcttaca attaacagac gctttggctg cctctacaat
    32161 agcaaaaaat gctcctgtag ttttagttga aaatggcagt aacaagagta ttttaagtgg
    32221 tgcagacaaa ttgactgtac taggaggcat aaatcagaat gttatcaagc aatgtataaa
    32281 tcaagcttca ccaaatcaac aaggtttata ttacaatcca aatgatagag catttaaaga
    32341 gagaataaag ggtaaagtat atgctcttac aaagcagtat agaaaagaga atggagtaag
    32401 agcgttatct gtagcaagca gattagaggg tttagcaaat gattggtcta atttaatggc
    32461 aaataagaag actttatctc atacgataaa tggaaaaaat tcatattcta cttttttgaa
    32521 atacttagat tggagtgaga ttaaacctgg atatatagca gttcaaggtg aaaatataat
    32581 taaatataaa attccagata aacctgtata tacaaataga gatgcagatg atataggaaa
    32641 ctttattttt aatgagtgga agacaaatcc agaagaagga actaacatgt tgcataaagg
    32701 gtatgaaata atgggatttg gtatagctat aacaggagat aagaatctat atgctacaca
    32761 tgaattttat ggaagatata aagaataaga aaatatacac aaaattatgc ttgtaaggaa
    32821 gctatataaa taattaaatt ataatggaaa ataacattta tattaataaa ttaatataaa
    32881 tgttattttt ttataattta atctaaaaaa attgtattaa aaagtttaaa tatagtataa
    32941 aatgggtaag aaagtagtga aaattaaatg gcaaaattag ggaaatctaa tgacgcaaaa
    33001 ctatagggat taaagctttt aaagccatat cagccagttg ctaaaaagag taaagtcttt
    33061 ttattttata attaagattc tctttgagtg tcttttttta ttgtctgaaa cttatagtat
    33121 ttaaattcat gaaaaattag atatttatta agggaggatt gttatttcat gaaaaaggca
    33181 atatcttgtg tactagcagt atctatgtgt agtgcaccac taaacgtttt tgcagaacct
    33241 atattagagg ggaagctaag ggcagttgaa aaatcagtta cagaaaagtt aagggggagt
    33301 cttgaagtag atttaaattt ttcactgcca ataaaaaata gcgattcaga aagtatgact
    33361 aatatgcagt tacgattaaa agatgatagt aacaatagtg gaataataaa gcttggagaa
    33421 aaagattcag gaacagtaaa tgttgggaca gataatatag aatatactat aaaaaaatta
    33481 tctgcaagta gaagtgaaat aaaaaataaa gatgagaata tatcatatta taatattgta
    33541 tttaataatt tacctgttgg aaaatacaat gtagaagtct ctggagatgg atttaagaac
    33601 aaagaaatta aagacattga tatatcaagt tattcacaaa gagtcttatt aagcaataga
    33661 gcaagtgtag ataataaaaa taaaccttta aattatgaat tgttcttaat gggtgatgta
    33721 aatggagacg gtaaagtaga taagagtgat tataataagg ttttagagaa tatagattca
    33781 aataaaagag aatttgattt aaatagagat ggaaaggtag atattgttga cttagattat
    33841 gtacagaaaa atttaggaca aagtgaagat gcaaaaagcc tagaaagtat agtatctacc
    33901 aatcctatag tagatacaag taaggttgaa ttaaaaggaa atagtgatgt agatattaat
    33961 ggtaatgtag aaaatttatt tagtggagaa aataatggag ttacaatatc ttcaaaaaat
    34021 gatgaaatat ctgaggaaaa accagcagta atggatatag ctttatcaga agcacttaat
    34081 atggaagtca tcaatataaa agctccagaa ggtactgctc cagcaaaagg aatagttgag
    34141 gttactgaca aagatggtaa tgttcaacag attccttatg aaaacaaaga atatatgaaa
    34201 aacttaaaag atgaggagca acaagaggaa gtaaataacg aacaagaaga agtaaatcaa
    34261 gaagaagtaa ataataaaga tgaaaatata aaccagactg ataataactc aaataataaa
    34321 gatgaaaata atgatgaaca acaggaaaat agcgataaac aagaagtaaa ctctaatacc
    34381 aatcaagata aaaataatga taaagaaaat tcatcaatgg ataaacctaa ttcagatgaa
    34441 gatactccaa aagaagaggg taatatcagt aaagatgata actctacaac atctacatat
    34501 agtaagtcaa cattagaaac atatgcagat gttcaaaata gtgatatagt aataaatctt
    34561 ggtaaacaag tagcagtaaa aaagataact ataaaaataa cagctacaac agcaaacgat
    34621 agaaatcttg cagaaatatc aaaagttgaa tttttaaaca atgtatataa agaaatacca
    34681 aagccagaga tgaatatacc taaaataaat aaaatatcga ctagcactgc tgttggaaat
    34741 gaaaaagtat ctttttattg gaataatgaa gtaaatgtat cagcatacga acttatacta
    34801 gataaaattg atagtaaggg aaaagtatta tctactaaaa aattacaaac cagtaaaaat
    34861 acaatagaag ttagagatat agaagcatat ggactatata gggttagcat acaatcttta
    34921 aatggagatt ggtctagtgg ttataaagat gcaacaccag aaaacttaga cggagtacca
    34981 gaaaatgtag attcaaacta taaacctata caatttagcc cagatagtat attagaattt
    35041 caagttgttc cagacacaaa accagaacca ccagaaggta taaatgttaa aggtaaattt
    35101 aaatctcttg atgtaagctg gaaaaatcat aaacaagcta aagattttga cttgtactat
    35161 aaagaaaaag gctctaatgg gtcttggata aaatacaatg ataagcttat tcgtgggaca
    35221 agttcaaaga tagaaggtct taaggataat acaacatatg aagttagaat gacagctact
    35281 aaccacttgg gaactagtgg aatgtctaaa acatatatag gtacaactga aagcatggat
    35341 gcaccaacaa cacctaacta tagattaatt aatactccta aagagaatgg tggatttgga
    35401 ataccaacaa atcacattgt agatgttact tatccagcag gatatgacaa aagtgaatat
    35461 agtgataaaa atccatttga taaattcaat gtagtagatg gggactttac aacacattgg
    35521 acattcccaa catataatgc agctgaggga acgaatagag gaccaataat aacttttgat
    35581 aaatcttata ctatggatac ttttatggct acacctcgtt ttgatgtttc tgataagaca
    35641 ataaatgact ttgaggtacg atattgggat gaaaatggtc aattacacaa tctgggggat
    35701 gttccttttg aaacaaagac agaccctgta aataataata agtatgtaat ggttagatta
    35761 aaagaaccaa taactgctac caagatacaa gtaagtgttt gttcatccta ttcatataag
    35821 tctaatatat cagaacttaa gttttatgag tatgacagta tagaagatga tgtaagaaat
    35881 ctatttatgg atgacctaca agttgaatta aaagaagatg taactcaaga aaaaataact
    35941 gaattaaaag atagattaaa tactccagat aaagctagtg gagaatatca tccatataag
    36001 acagttattg agagagaatt aaaattagct caagacttat ataatgatag agccacactt
    36061 actgatgtaa ctactgtaaa tcaagaaata aataaattag gaaaatataa agataaaaac
    36121 ggacagtata ctgtaaattc caataattta ggtatgcaaa atgactggca ggcattagga
    36181 gtagctgcta gagcaggaga tgaaatcaca gtatatgttg gaagtaaaag tggaaaaact
    36241 cctaagttga tatatacaca gtactatgga gaatctggtg catataagag tggagaaata
    36301 aatcttaaca ctggaaaaaa tgtaattcaa ttaagcaaac ttcatagttt agatatagaa
    36361 tgtggaggaa gtttatacat aagatacgct gaagatactc caagtggagg agatataaaa
    36421 gtacgtgtag caggagctac taagatacca caccttaatc taaatggaac aataaatgat
    36481 aagagtcctg cgggagtttc tgaatctaag aaaaagataa aagcatatat agaagaatta
    36541 gctagatata aagatgctgt aaaaggagaa ccatattatc caggaacaaa tgttggtaat
    36601 tcatatggat attcagaaaa aactggtgtg ttaaacacta cagatattga aagtgataaa
    36661 gtaacattaa atgttcctgc aacagctgta tatcaaggga tatcaaaagg aaatgctgat
    36721 ttaaatacac aagtagatag attatataat agtatgcttg catgggaaca aataatggat
    36781 ttagtttact cagaaagagg agtatttaaa acacaagatt tagatggtaa tggaactgta
    36841 gatgataatg aatttaactt aactaaaaat gatatggctc caaaatctcg tatgaacata
    36901 aaataccaaa gaatgtttat tggagcattt atgtatgctt ctggattaca tgtaggagta
    36961 gaatatggtt cagttccagg gcttatgaat ggagtacctt tccaaataga tgatagtgga
    37021 aaagctacag gaggaaactt atttggatgg ggaataggtc atgagatagg acatgttaca
    37081 gatattggaa agatgacata tagcgaaact agtaacaatg tacttgcttt acttgctcag
    37141 acctttgatg ataagacaca ttcaagaatt gaaggttcaa caatggataa gatatatgag
    37201 catgtaactt caaatagctt aggtatacca agcaatgtat ttgaaaggct tggaatgtta
    37261 tggcaattac atctagctta tgatgatgat tttacgggtt caatgttaaa gaataactca
    37321 gatgctgatt taagtaatga tacattctat gcaaagattt caagaaaata cagagcactt
    37381 tcaagtagtg acccaattaa tagtttgcca aaagaccaaa tgctagtagc aatggcatca
    37441 agtgttgttg aaaaagattt aagagattac tttaaggcat ggggagtaga aattacacca
    37501 gaattaaaca gtataatgga tagtaagaac tacgaaaaag aatcaagaaa gatacaatat
    37561 gtaaatgacg aagctagaag aaagatttta aatgaaaata tagctagtat ggcaaaagat
    37621 actaaagtaa gtgctagttt ttctgatgga atacaaagtg gttctctagt taaatctaat
    37681 aaaatatcta tagacctaga tgtagataag gataaagata agatactagg atacgaaata
    37741 ataagaagtg atggaaatta tttagatggt gaaaatggaa cacaggtaaa atacagaaca
    37801 gttggatttg tggatgcaaa aggaaatagt aagactaaat ttactgataa tatatctcct
    37861 cttaataaca gagcatttac atataaggta gttgcatatg attatcacct aaatccaaca
    37921 gaagaatttg aagttggtac tgtaaaatta tcagatgaag ggaaaataaa taaatcagca
    37981 tggtcattca taacaaatac tgtttctgac ggtgatgtta gaactgaaaa tgactcacat
    38041 ggaccattac aaaatcctga aattgataat ataaaagata atgatgcaaa tactacatat
    38101 aaaggaaaga taataagtaa agcagagtgg aacaaagacc ctcaaaaaga ccctgatata
    38161 aatgtagagg aaaatccata tataatagta gacatgaaag aaacattacc tatagtagga
    38221 ctaaaatata caaaaccaga gacagaagct aggaagttct cattaaaagg attatttaac
    38281 tttagaaaga atactagcac aacatacaat cctttaacta attacaaaat acaagtaagt
    38341 aatgataaaa agaactggga agatgtaagt caaggaactt ttgaatatgg acaagattta
    38401 gttggtggag gaaaagacca agataatgaa gctagagtta catttaataa agatggtaaa
    38461 ctttggacat accaagctag atatgtaaaa ctaatctcaa ataataagtc taatatagaa
    38521 gttgcagaat tagatataat aggacctcca ggtgataata tagaaattgg cacaatgaat
    38581 aatgcagacg aaactcgtac taatggaatt ggaaaattag cagaagatta tgtatatcaa
    38641 agtgatgact ctagcacaag tgaagatgaa acaggtaaga ttccagcggg ttcaatagtt
    38701 attacaggtg aatacagtgg aaatccagca tttaatttac cactacttat agataaaaac
    38761 aataagacta tatctggaga agcactttta tttgcagaag ttccagaaaa aggtgaacta
    38821 ggtgaaatca gtaaaggtac atgggtttac tatatgccaa aagaaaactt tgattcttta
    38881 agtgataagg ttaaggctga actatataga tacaatgacc ttcaaggaga tactccagta
    38941 ggtcaaagat ttgtaagtga tacaatgtat gtacctgtag gagctaagaa ttatgatgag
    39001 ttaaagacaa taagtcttac tgatagtaat agtaaagcta gaaaagctac aagaaattct
    39061 attgatatca gtaataagaa ggttataact gtaagcaata aagtaaaggg taatattgtt
    39121 agagataaat agtaggtgga actctaaaaa cgtaaaaata ctaacactaa cattgtatat
    39181 ctaataagat taggtactag aatagtttta aaatagtaga tattaaaagt aaagtatata
    39241 aatgtaatca taaatttagg gtaatttata aattaaatat aaaagctagg tggataagta
    39301 aatttatcca cttagttaat gatagataag gacgggtgtt aatgagtaaa agaaaatctt
    39361 ttataagaac tatagcagta tcaactatgg cagtagctgt aactggtagt gctacatgtg
    39421 catatgcagc tccagtttta caaggaacaa aaacgtatga gaaagtaaat actatagata
    39481 tatctgtgga ctcagtagaa aacatagtat attcatttca agccagtata aaagtacaag
    39541 gtgaggttga agtagtagat aatgagcaga aagaaaagat tacttggtct gataatataa
    39601 aatctcagat aaaaagtgga aatgcaaatg ctacttgtag agcagaatac aacaaatcaa
    39661 gtaatacaac tacattagat atatatgtta catcaaatga agacttacta gacggaaata
    39721 gattgaatat aggaagaata tctgtaaaaa aatctggaag taactcaaat gctgactata
    39781 aagttttagg aaaaggaaca aatgataaac cagctttaaa aattgtaaca tataacaaca
    39841 aaacagtgga ttatgaaaat ataagctctg atgaaggatt aatatttacc cttattaatg
    39901 agagtgaagt aaaaccgatt gggggaactg gttctagtaa aaatgaccct gaaaagtata
    39961 aggtagagaa aagtgaagca cttgaatatt tattaaacaa tattaggata aactattcta
    40021 ttgttagtaa agaaacacaa gaaagtggtt caaatgtaat attaaaactt ggattagcac
    40081 aaaaaacaac taagggaaga aaagttacaa taaacaaata tgttgaagtt acacttccaa
    40141 aaagtttaga atacatagtt gaaaatgaat tatcaaaacc ggatgaatta ccaccagata
    40201 atggtagtgg tggaaataat ggtggaggaa gtaattcagg cggctcatct agtggtggaa
    40261 gctctggagg aggaaattca agtgattcta catctaatgt cacagtcaaa aaattaaaag
    40321 gtgatgatag atttgagaca gctatcaaaa tatctcaaag tggttggaca aaatcagata
    40381 cagttgtaat agtaaatgga gaagataaaa gtatggtgga tgggcttaca gctacaccac
    40441 ttgctagtgt aaaaaactca ccgattcttt tatcatcaaa tgataaactt cctcaaaaaa
    40501 ctgtagaaga attgaaaagg ctaaatccat caaaagtagt tgtgataggt ggaaacaact
    40561 caatgccaaa ttcagttgtt gaagctatca aagctgtaaa ttcaaaaata tcagtacaaa
    40621 gaataggtgg agatacaaga tatcaaactt ctattaatat agcaaaagaa atagataaga
    40681 cgaataatgt aagcaagttg tatataggag caggaaatgg agaagctgat tcattatcaa
    40741 tagcatcact ggctggtaaa gaaaaaactc caatagtact tacacaaaaa gatggtgtag
    40801 ataatgaagc tgaacaattt attaaatcaa ataaggtgtc aaatatatat tttattggtg
    40861 gagtagaaaa aatatctaac aaggctattg aacaagttgg aaaaatagca aacaaagata
    40921 tatcaaacaa tagggtggca ggtcaaacta gacaagagac gaatgccaaa gtaatagata
    40981 aattctactc tcaatctaag ctagatggag ttgtagttgc aaatcaagat aaacttatag
    41041 atgctttagc agttggacca ttagcagcta aaaacaattc accagttata cttgctacaa
    41101 atacgttaga taaatcacaa gaatcgtcat taaaaggtaa aaattcatca aagttgtttg
    41161 aagtaggtgg aggaatagcc tcatctgtaa tagacaagat taagagttta attgaaaaat
    41221 aaatattaag taaatagaaa ataagaatgt atttagatat a
//
LOCUS       DAYAOY010000028        41098 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYAOY010000028 DAYAOY010000000
VERSION     DAYAOY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.028"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            213..2714
                     /locus_tag="ACGI6B_001561"
     CDS             213..2714
                     /locus_tag="ACGI6B_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9751008.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2874..3542
                     /locus_tag="ACGI6B_001562"
     CDS             2874..3542
                     /locus_tag="ACGI6B_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751009.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3526..4803
                     /locus_tag="ACGI6B_001563"
     CDS             3526..4803
                     /locus_tag="ACGI6B_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9751010.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5204..5425
                     /locus_tag="ACGI6B_001564"
     CDS             5204..5425
                     /locus_tag="ACGI6B_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751011.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5714..6478)
                     /locus_tag="ACGI6B_001565"
     CDS             complement(5714..6478)
                     /locus_tag="ACGI6B_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9751012.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6752..7426
                     /gene="fsa"
                     /locus_tag="ACGI6B_001566"
     CDS             6752..7426
                     /gene="fsa"
                     /locus_tag="ACGI6B_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFG9751013.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7548..8942)
                     /locus_tag="ACGI6B_001567"
     CDS             complement(7548..8942)
                     /locus_tag="ACGI6B_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFG9751014.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(9020..10366)
                     /locus_tag="ACGI6B_001568"
     CDS             complement(9020..10366)
                     /locus_tag="ACGI6B_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9751015.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      10616..10718
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10812..12188
                     /locus_tag="ACGI6B_001569"
     CDS             10812..12188
                     /locus_tag="ACGI6B_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFG9751016.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(12302..13447)
                     /locus_tag="ACGI6B_001570"
     CDS             complement(12302..13447)
                     /locus_tag="ACGI6B_001570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9751017.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            13700..14407
                     /locus_tag="ACGI6B_001571"
     CDS             13700..14407
                     /locus_tag="ACGI6B_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751018.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            14454..16049
                     /locus_tag="ACGI6B_001572"
     CDS             14454..16049
                     /locus_tag="ACGI6B_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751019.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            16152..17234
                     /locus_tag="ACGI6B_001573"
     CDS             16152..17234
                     /locus_tag="ACGI6B_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HFG9751020.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            17383..17544
                     /locus_tag="ACGI6B_001574"
     CDS             17383..17544
                     /locus_tag="ACGI6B_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HFG9751021.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            17885..19153
                     /locus_tag="ACGI6B_001575"
     CDS             17885..19153
                     /locus_tag="ACGI6B_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9751022.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            19348..20136
                     /locus_tag="ACGI6B_001576"
     CDS             19348..20136
                     /locus_tag="ACGI6B_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9751023.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            20136..20585
                     /locus_tag="ACGI6B_001577"
     CDS             20136..20585
                     /locus_tag="ACGI6B_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFG9751024.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            20589..22853
                     /locus_tag="ACGI6B_001578"
     CDS             20589..22853
                     /locus_tag="ACGI6B_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9751025.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            22993..23304
                     /locus_tag="ACGI6B_001579"
     CDS             22993..23304
                     /locus_tag="ACGI6B_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751026.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            23590..24090
                     /locus_tag="ACGI6B_001580"
     CDS             23590..24090
                     /locus_tag="ACGI6B_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFG9751027.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            24147..25133
                     /locus_tag="ACGI6B_001581"
     CDS             24147..25133
                     /locus_tag="ACGI6B_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751028.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            25183..25905
                     /locus_tag="ACGI6B_001582"
     CDS             25183..25905
                     /locus_tag="ACGI6B_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HFG9751029.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            25934..27868
                     /locus_tag="ACGI6B_001583"
     CDS             25934..27868
                     /locus_tag="ACGI6B_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9751030.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            27861..29129
                     /locus_tag="ACGI6B_001584"
     CDS             27861..29129
                     /locus_tag="ACGI6B_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFG9751031.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            29456..30496
                     /locus_tag="ACGI6B_001585"
     CDS             29456..30496
                     /locus_tag="ACGI6B_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751032.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            30852..32174
                     /locus_tag="ACGI6B_001586"
     CDS             30852..32174
                     /locus_tag="ACGI6B_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFG9751033.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            32471..33832
                     /locus_tag="ACGI6B_001587"
     CDS             32471..33832
                     /locus_tag="ACGI6B_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9751034.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            33905..35299
                     /locus_tag="ACGI6B_001588"
     CDS             33905..35299
                     /locus_tag="ACGI6B_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9751035.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            35361..35831
                     /locus_tag="ACGI6B_001589"
     CDS             35361..35831
                     /locus_tag="ACGI6B_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFG9751036.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            35834..38050
                     /locus_tag="ACGI6B_001590"
     CDS             35834..38050
                     /locus_tag="ACGI6B_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HFG9751037.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYALSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            38133..38804
                     /locus_tag="ACGI6B_001591"
     CDS             38133..38804
                     /locus_tag="ACGI6B_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9751038.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            39167..40381
                     /gene="dpaL"
                     /locus_tag="ACGI6B_001592"
     CDS             39167..40381
                     /gene="dpaL"
                     /locus_tag="ACGI6B_001592"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFG9751039.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            40508..>41098
                     /locus_tag="ACGI6B_001593"
     CDS             40508..>41098
                     /locus_tag="ACGI6B_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9751040.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT        15953 a         4434 c         7194 g        13517 t
ORIGIN      
        1 tatatataca tttaattatg agtatacttt tctattaaat acaaataaat ataactaata
       61 catttattta ttaaaaaaat attgatttaa tatacctata gggggtataa taaaaatata
      121 gatttctaaa taagtaattt atcaatatta catatcaaaa taaaacgtta caaattagct
      181 gaaaatatat tgaaagaaag gaagatttta ttatgagttc aaataaagtg gtagaaagtt
      241 ataaaattac aggtatgaca tgtgcagctt gtgcaaaggc agtagagaga gtaactaaga
      301 agatggatgg agtatatgac caaagtgtaa atatagctac tgaaaaactt aaaatagaat
      361 atgataattc taaggttaat tttgatgata taaagcaggt tgtagaaaaa gcaggatatg
      421 gaattataaa ggaagaaagt aataaaaaaa ttgatatgaa aattgatgga atgacatgtg
      481 cagcttgtgc aaaagctgta gagagagtgg taaaaaaatt ggatggagta gaaagtatta
      541 gtgtgaatat tgcaacagat aaagctaata tagactatga cccatcaaaa gtaaaattat
      601 ctcaaataaa agcagctatt gaaaaagctg gatataaacc aatagaagag gtaaaaaata
      661 aggttgatgt ggatgaagat aaattgagaa aagaaagaga aatgaagtct ttatttgtta
      721 aatttatagt tgcaatagta tttgcagtac ctctgtttta tatagcaatg ggacctatga
      781 taataaagcc tatagggcca tggcctttgc cagaaattat aaatcctatg acaaatactt
      841 ttaattatgc attaattcag ttaattttag taataccagt aatgatagct ggttataaat
      901 tttatataaa tgggtttaaa tcactttttt ctttaagccc taatatggat tctttagttg
      961 caataggaac attagccgca tttttatata gtctatatac gacactacaa atagccaatg
     1021 gacaaattca aggtatgcac catcatcagt tgtattatga aagtgcagga ataataatag
     1081 cacttatctt acttggtaaa tatttagaat caaaatcaaa aggaaaaaca tcagaagcaa
     1141 ttaaaaagct tatggggcta caacctaaaa ctgcaatagt gttagttgat ggtaaagaag
     1201 ttgaaactcc tatagaagaa gtagagatag gagatatact attagtaaaa ccaggaacta
     1261 aaatacctgt ggatggagtt gtaatagaag gatatacatc agttgatgaa tcaatgctta
     1321 caggtgaaag tataccagta gaaaagaatg ttggaagtaa ggtgacagga gctagtatta
     1381 ataaaaatgg tgttattaag tttaaggctg aaaaaattgg tggagatact gctcttgccc
     1441 aaataataaa gttagttgaa gatgcacaag gtacaaaagc acctattgct aaacttgccg
     1501 atactgtatc tggatatttt gtaccaatag taattgcaat agctgtagtt gcatcattat
     1561 tgtggttttt aataggagga aaagatatag tatttgtact tactatattt atatcagtac
     1621 ttgtaattgc ttgtccttgt gcattaggat tagcaacacc aactgctata atggttggga
     1681 ctggaaaggg cgcagaaaat ggaatcttga taaaaggtgg agaggcatta gaatctgcac
     1741 ataaagtaaa tacagtgata tttgataaaa caggtactat aacagaagga aagccaaaag
     1801 taacagatat agtattaaat aataatgtaa aagaagaata tcttatcaag attgcttcaa
     1861 gtgcagaaaa aggttcagaa catcctcttg gagaagcaat agttaagtat ggagaagaga
     1921 agaatataaa atttgaaaaa gttgataatt ttaaagccat accaggtgct ggtatacaag
     1981 ttactattaa tgatgaaagt atattacttg gaaatagaaa gcttatgaat gacaataata
     2041 taaagcttgg ggatttagaa gaaaaatcaa atatattggc aagccaaggt aaaacaccta
     2101 tgtatatagc tgtagatgga aatttatcag gtataatagc agttgcagat gtagtcaaag
     2161 aaagtagtaa aaaagcaata gaaatacttc atgatatggg aataaaagtt gcaatggtaa
     2221 ctggagataa tgcgaaaact gcaaatgcta tagcaaatca agtcggtata gatatggttt
     2281 tggcagaggt tttaccagaa gataagtcaa aagaagttga aaaactacaa aatcaaggaa
     2341 agtttgttgc aatggtaggg gatggaataa atgatgcccc agctcttgca aaagcagata
     2401 tagggatagc aataggaagt ggaacggatg tagccattga atctgcggat atagtactta
     2461 tgaagagtga tttaattgat gttccaacag caattaaact tagtcatgaa actataaaaa
     2521 atataaaaca aaacctattt tgggcatttg gatataatac aattggtata cctgtagcag
     2581 caggaatatt atatgtattt ggaggacctt tattaaaccc aatgattgca gcagcagcaa
     2641 tgagcttaag ttcagtttca gtcgtatcaa atgcacttag acttaaaaac ttcaaggcat
     2701 ataagagaga ttagattaat aaattaaata aaatgtatcc aaaagagcta tctttaaatt
     2761 aaaaggatta atttacaaat taagatagct cttttttcat gtaaaattca caacaataag
     2821 ataaaatata agtaatatac gaaattttaa ataaaataga aaggattgat agaatgaatt
     2881 cgtcaatact tgtaattgaa gatgattcaa atatacaaga gttaatatca gaatttttaa
     2941 gtgcagaagg ttatcaagtt gatacagcaa atgatgggtt ggaaggtata caaaagttta
     3001 agcaaggaag ttatgattta gtaatattag atataatgat gccaaactta gatggttatg
     3061 gagtttgtaa aatgataaga aagtcatcaa gcgttccaat tatattttta actgctttaa
     3121 atgatgaagg agaccaactt aaagggtttg acttagaatg tgatgattat ataacaaaac
     3181 cattttcatt taatcttctt ataaaaagag tagaagcaat tttaaggcga agtaacaaga
     3241 ctataaatga caaatttata gtttttgaaa aattaaaatt agatttaaac acatatattg
     3301 cagagataga tggagaacct atagaactta cactaaaaga gtttaatata ttaaaagctt
     3361 tgatagaaaa gtatccacaa gttataacta gggaaggtct tttggatagt atatggggtt
     3421 atgactatta tggagatact aggattgttg atgcacatat aaaaaatata agaaaaaaaa
     3481 tatcattacc atatattaaa actgtaaaag gaataggata tacacttgag aaggatattt
     3541 gataagtggg aaaagttaag tataaagtat aagttatttt caataacgac atcattatta
     3601 atagcattag ctttaataat atatctaatt ttatattttt tattgccatc atattatcat
     3661 gagtataaaa ttgagtctct tcaagaaagt ttgaaatctt tagtagatag ttcaattcat
     3721 ttcgatacat atactttaga agaaagactt tattatatgg ctaaagacca aaatttggct
     3781 attttattaa aagataacca aggtaaaatt gtatatggta agaatgaagt agttatttta
     3841 agatatagta agtacatgat taatagtctt gaggatgagt atagaacttc tatacctata
     3901 tatactaaag atgcaaaaga tggtccatat actttagaac tcgttatgcc attacaacca
     3961 attgacgaag caaacgaagt tataagaaaa ctaatgccat acatcatatc aatagcaata
     4021 ttgatagcga ttattggagc atatatatat tcaattgtaa taacaaagcc tcttataaac
     4081 ataatagaga gtgagagaga acaggaatat agaaggaagg attttgttgc tacaatatca
     4141 catgaactaa aaactccaat aaccataata agtggtcaaa ttgaaggtat gatttatagt
     4201 gtaggtaagt ataaggatag ggatacttat ctgaaaaaat cgtatgagtg tacacaagaa
     4261 ttaaaagatt tagtaaatga aatgatagag gtttcaaaat cagaaatact tgaaaaagat
     4321 ttgaaattgg tatcaataaa tataagtgaa ttattaaata gacttgtaaa gagacaagta
     4381 tttttaatag aagaaaaaca tatgaaaact attttaaaaa tagaagaaaa tttggaaatt
     4441 aaagcagacc aagagagaat aactaaggca atcaataata taataaataa tgccataaaa
     4501 tattctcctg aagaatcaga gcttataatt agactctatg ataaaaataa acgaataagt
     4561 aaaaagactt ctaatcaaag agtggtttta gaaattgaaa atactggtgt aactatcgag
     4621 aaacgttatt tagaagaaat atttaatcca ttttatagaa tagaaaaatc tcgttcgaga
     4681 aaaactggag gaagtggact tggactttat atagtcagcc aaatttttaa aagtcatggg
     4741 tttgattata gcataaaaaa caaagaaaac tctgtagtat tcacagtaga atttaaaaat
     4801 taattactta ttataaagaa tatataactt tcaataatag ttcattttaa aatcacaaaa
     4861 aattcataat gaaactgtat tatataaata taaataagct taaaaccata aaatacacca
     4921 actataatga tgagatttcc ttacatcctc catataagtc atcattatag taacccaaca
     4981 attaagttac tatgaattta aaaaataaca ccgagcatat aatctatgct tagtgttatt
     5041 tttattttga aataaaaaat atttaattta ttgaaatgta tagatttaga tagtttaaac
     5101 tatatttaag acttaaaaat tataattgat attacaaata aatatatgta ctttgggaaa
     5161 aataaatata ggaagtatta tatttacaga ggaggtagtt ttgatggata gattaaaaag
     5221 agatatgaaa aatatgaaag aagatttaaa agaaggtgtt aaaaagacag cttatgttat
     5281 gaaaaataca gctgaagatt tgaaagatga tgcacaaggt gctatggtaa acttagaaat
     5341 gaaaaaagat gagatgatgg aaagatatga acaaaagaaa ttttctaaag aattagagaa
     5401 gcaaatgaag aatgaaatga aataagcttt tatttatgaa taacaaatta ttgtattcat
     5461 ataaaaagta gccagtaata ttaaatagtt tatttaagga aatagacttt ctaaaaataa
     5521 ctgtattaga tttagaaagt ctatttcaaa ttagttttat aaatagacat aaggataaag
     5581 tagatgttat agaactagat tttaaaaaat agatactaag gataaaataa atattataga
     5641 aatatgtttt taagaatatt tatcaaataa aaatttatat ttataagaaa tcctataaaa
     5701 catataatta aacttataaa acatttacat taaattctga taaaatatct actatatcag
     5761 gagtaggcgt tttttctgta attatagaac taatatcttc taaatgagca aacttgaaat
     5821 ttccatcaaa tttaaacttt ttatcttcca taactataaa tactttttta cttgaattta
     5881 ttatagcctt cttcgtaaga ccatcttcaa tttcaaaagt tgtaagtgaa gagttgaata
     5941 catctactcc acaacttccc ataaaagtct tatcaaactt atatttcgat ataaactcta
     6001 tagttggagt accgacaaaa ccgtctaaac tgagatttaa taaacctcca gtggaaatta
     6061 tatttaaatt atttggtttt gaagtaacta catttagtat atccagcata tttgtaacaa
     6121 gtgttacttt tttattactt tcagctaaca acttcgcaag caagatgtta attgtagaga
     6181 tatctaaaaa tatagtttct ctatcttcaa ttaaattaaa tgctttttct gctattattt
     6241 tctttgttgg aatattaatt tcttttctgt cttttgtatc ctgattctct agtgtttctc
     6301 tagctagtac agctcctcca taaattcttc ttattgaaga aatcttttct atatttttta
     6361 aatcttttct tatacaatct tctgtaacat taaatttaga actgagctct ttgacagtaa
     6421 ctctaccttc tttttttagg atatttaata tagcttggat tctttcttct gcaaacattt
     6481 tctacctctt ttcgcaatat agtattatag taaatattat aagataaaat acaatatatc
     6541 aactatttta acatgaaata caaaattaat ttaatataaa aagtatgttt tttctaacta
     6601 tatatttaga atttagtaaa taaaaaaatt ataaaactaa gaaggaaaag taaaacaata
     6661 ataaaaaata aaaaacaata agcaaaatgt tgaaaataat accaaatcaa tatataataa
     6721 gaataaagaa agattaaagg gggatatttt aatgcaacta atcttagata caggtaatgt
     6781 ggaggagatt aaggagttat gtacttgttt accaatagat ggtgtaacta caaatccaag
     6841 tattgtttca aaagaaaaaa agaattttaa gcaacttata aatgaaattg gtgagattat
     6901 aggtgaggat atgcctatac atgctcaagt gttaagtaca aagtatgaag aaatattaga
     6961 agaagcatta tatataagta gtttgagaaa aaatatatat gtaaaaatac ctgtaacaca
     7021 agatggttta agagctataa aagaccttca cagaaaaggt gtaaaaataa ctgctactgc
     7081 aatattcact gctcatcaag gttttttagc tgcaaaggct ggagcaaatt atattgcacc
     7141 ttatgtaaat aggcttgata atatatctgg tgatggagta gctatggttt cagaacttat
     7201 aaaaataata gatacttata aaatggatac aaaagtactt gcagccagtt ttaaaaatgc
     7261 acaacaagtt atagaattaa tgcaacatgg ggtgcatagt gttacagtac catatgatat
     7321 ttgtaaatct atgatgaatc atccattaac tgattggagt gtaggtaagt ttatagagga
     7381 ttgggagaat acttttggaa aaggaagtaa aacaaataat atttagtatt atggtaaaag
     7441 agtagttttg tccaatttca caagaactta tataaaaaga tgaaaatatg aattttcttt
     7501 ttaataattt cgttattatc ttaagttaat tcatattttc atctttttta gatttctagt
     7561 cctataaatt tccaccaagg gaatgttata catattatta taaagtaggt taaaggaatc
     7621 aaagttaaac cgtatttgaa aataaccttg ctattataca gattttcacc atgaccaacc
     7681 attattgtag catgatgaaa tggtaatatg tactgtatgc ttactataat gtagacaaat
     7741 agacataaag taacactatt ttcctttagt atatgtaaac ttccaagtaa cggtattact
     7801 acagatgatg tagtgacagc acttccaaga catatattca agattattgt gagagttatc
     7861 aaaaacaata ttgaaataat ttctgaagag tttggtataa actttatcaa ataatttccc
     7921 attatatcta caactccaga gtactttaac actcctccaa ttgaaaatgc tgctataaaa
     7981 aaaataagga ccttccaatt tatttctttt aaagtactaa atccaactat ttttcttagt
     8041 aataaaagaa gtacacaaat tgacattata attaatgtgt ttatgttatg tatgaactga
     8101 gttaaaaaca taaaaaacat tattcctagg cataaaaata cgtgtttttg ctctttgttt
     8161 agcaaattaa attctttttt taaattagaa tatgaaaaat gaagtttatt accctcaaga
     8221 ttttcattag aaataaattt tattgaatgc aattcttttt taaagacaaa gataaataaa
     8281 aaataagtta taaaacaagt aattatagtt ggaacagaca tataaaaagc ccattgaccc
     8341 caatttatat tacaatttcc aagttctagt acaacataat ttagaagggt gtctccattt
     8401 acaaaaaaca ttgatgtaaa ggtagatgct gtaaatatac taaataaaag tatagatttt
     8461 gaatcttcag ttatctgttg ttcttttaaa aactccttgt aaaaagcaga tacaagaata
     8521 acccttggaa atggttgtgg gataaaaaat attaaaagta tacctaaaat atacgataaa
     8581 agaatcattt ttttaggggt atttacagta tttaaaatta acttctcaga aataattttt
     8641 gcgacacctg tttttgtaac tgcattagta attatgtatg caagaattat tatgtaaaaa
     8701 ttttctgtaa ataaaaactg taaaataacc tttattggtg caatgttaaa tataaacgct
     8761 agaattacaa aaaacaatgc aacaaaagtt ttttcaactg agtctacagc ccatatcaag
     8821 atagttaaaa aagataagca tatagaaaat tttatgttga aactgtaatt aagagagaag
     8881 tagagaatta tggtagctat aacagtcata taaaattgtt ttggcttaag aataataccc
     8941 atttttatat tcacctccat aatttattat atcataatgg aggtgaatac aatgaatgtg
     9001 aatacatttt ataaaaagat tattcattga ttctatgatt tacaatagct ttagttttcc
     9061 aatgtccttt cttatagtag ataactgtaa caataagacc taaagtccaa ccaaaaggaa
     9121 tacccatcca aattccgtct gttccaaaaa atttagaaag catataagaa gctggaagtc
     9181 ttccaagcca taaagatact attgagctta taagtgcaaa catggaatca ccagcacctt
     9241 tcattgcact tgaaagtaca aaagaagttc caataaataa gaagaatggt cctattataa
     9301 ataagtaaga agagccaatt ttaatgacat ctttatcagt attgaataga gcaattagat
     9361 tttccctaaa tagcaaaatt aaagcaataa ctgtaactgc taaaactaag gtcattttta
     9421 tagattctcg aatgcctttt ttaactcttt cattttctcc agcacctatg ttttgagcaa
     9481 caaaagtaga aacagcagaa ccaagattca taataggtaa gcttataaaa ttttctatcc
     9541 taagaccagc accaaaagct gccatagcag atgtaccata actattaact aaaaattgaa
     9601 gagccatatt tccaattgaa aacaacattt gttgaactcc acttggaagt cctaatctaa
     9661 gtccatcttt aaatattttg ttatcatata caattccttt gattttaaat ttaagcactt
     9721 catgtttttt atttaaataa attatactta ttataaatga tactgcctga gccattatag
     9781 tggcaagtgc aacgccctca acacccattc ttagcactac aataaaagtt aagtctaaaa
     9841 ctatatttag aattgtggct attatcagaa aataaagtgg agttactgaa tctccaagtc
     9901 ctctaagtat tgcacttact gaattatatc cagcagaaaa taatattcca ctaaatatta
     9961 tagtaagata caattttgat tgagcaaaaa cggattctgg tgtattcata agcttaagca
    10021 ttgggccacc aaatactatt ccaagaatac ttattaatat agaaccaata aataaaagta
    10081 tatatgtagt atcaatagta gcttttacct tttttaaatc tcctgagcca taaaattgtg
    10141 acactaaaat agaaaatcca aggctaacac ccataagcgc tgccaccaat aaaaacatta
    10201 tagggttagc tccagagact gccgccattg cttcttttcc gataaatcta ccaactatta
    10261 tactgtcagc tgtattgtat agttgttgaa ataaactacc aataagcata ggcatggcaa
    10321 aaaagaatat ggactttccc tcatgacctg tagttaaatc tttcacctta ttcctccaaa
    10381 taaaaaaatt attaatttat tatagtataa tcttaacata aactttatat ccaaactata
    10441 aataaattgt atcaaaatat catatatttt tttaaaatgt aaagaatata attataattt
    10501 gaatttaata aatcaatatt taatataatc gaatattaat acaaaatcaa tacaatatat
    10561 acgtatatat tgacaaaatt aattatataa attaaaataa tattcgtaaa gaaaaattca
    10621 tgttttgtac tgtataacat ataattttga caatatgggt cataagtttc taccggaata
    10681 ccgtaaatat tctgactatg tatactatta tttagttaat taaatattta attaactgaa
    10741 ttatgaagga tacggctcag cttatcaact actgtgtcgt ttttttgtaa aatttaatag
    10801 gaggtgttgt tatgcaaaca acaaacagta gtactatgca gaaacttttt ccaattttga
    10861 ctaataaaaa tgtcgacatg aagaaggaaa taattgcagg agtaactact tttttaacta
    10921 tggcttatat aatagctgta aatccaaata ttttatctga gacaggcatg cctgcaggtg
    10981 cacttgttac aggtacttgt ttagcagcag catttggatg tattttaatg ggtgtagttg
    11041 ctaaccttcc ttttgcttta gcatcaggta tgggtcttaa tgcatttttt gcatatacag
    11101 tagttttaca aatgggtgtt ccttgggaag tagctcttac agcagtattt gtagaaggta
    11161 ttatttttat agtattatca gtaagtggag ttcgtgaggc tgttgtaaat gcgataccaa
    11221 aaaatatgaa attagctgtt acaggtggta taggaatatt tattgcactt ataggtctag
    11281 ttaacagtgg aattgttatt ggagaccaag ccactcttat caaaatgggt agatttacac
    11341 cagcagtaat aataacttgt gtaggattaa taattattgc tgtattagat aaaaaaagag
    11401 ttaaaggttc tatacttttt ggaatagtag tgagttcatt acttgcttgg gcatttgctt
    11461 ttataaatcc agagtatgca caaaaattag gtatatattt acctggtgga ttatttaaat
    11521 tcgaaagttt agcaccaata gcaggtaaaa tagatttagg atatgtacta catccaacaa
    11581 atataggtgg ttttttagta gtagtttgta catttttatt tgttgatttt tttgatacag
    11641 taggtacatt agttggtgtt tgttctaaag caaacatgtt agatgaaaaa gggaatgttc
    11701 ctaatgtagg tagagcctta cttacagatg cggttgcaac tacagttggt gcaggacttg
    11761 gagtatctac agttacaact tatgttgaga gttcaacagg agttatcgca ggtggaagaa
    11821 ctggatggac agcaataaca gttggaattt tattcttagc tgcaatgttt ttctctcctg
    11881 tatttatagc aattccatca tgtgctacag caccagcttt aatatatgtt ggatatttaa
    11941 tgcttggtac agttaaggat atagagtttg ataatataac tgaaggtgtt ccagcttttg
    12001 ttactattgc ttgtatggca ttaacatata gtataggtga tggtttaact ttaggtatat
    12061 taacttatgt tttcgtaaat atattttata atatatttgg agctaaaaag gcagaagaca
    12121 aaaaacatgt ttctgtagta atgattgttc tggcaatatt atttataata aaactttggc
    12181 tactataata ggtattattt aaaattttaa tttaatataa aataaagggc tgtaatttat
    12241 tatcaattgt gatgataaac tacagctctt ttgtatttgt tgtatttttg tatttattgt
    12301 attatttaat aatgtctatt agttctccat tttgtaaagt tttaggtgta attactatat
    12361 tgaactcttt ttgcaaatct tttataatat ctaattcttt ttttgttatt attgagattg
    12421 tgttaccact tcgatttcct ctagcagttc gaccacatct atgcaggtat tcatttttac
    12481 tttttggaaa atccaagtta aaaacatgac ttacatccac aatatctagt ccacgagcag
    12541 ataaatcagt tgtaattaaa atctttgctt taccaagttt aaatttattt attgcattct
    12601 ttctatcttc tttttccata tttccaaaaa ttcctattgc tttgtaatta tgataattta
    12661 gttttgatac taggacttct atattttttt cattatttac aaatacaata gctttttttg
    12721 gatttgtagc tgctaatgcc tttctaagaa aagtaaattt atcccttatc tcacctaata
    12781 aatatgagtg attgatgtta ggatttattt gtgatttttc ctttgttttt attatttcaa
    12841 actccttcat aagtttatca caaattttta tagtgctatc agtaagactt gcagagcatc
    12901 ctataatttg tctatctctt aacgttgttc ttattatgtc ttctatacaa gttatatttt
    12961 ttccattcaa tagattgtcc acttcatcta agataatagt ttttatatta tgtgatttta
    13021 gtttcttttg ttttatcaag tctaaaactc ttccacaaga gccaattact atgtgtggtt
    13081 ttactgcttt tatatttttt atttgttttt gtatattgac ctccccaatt aaagctaaag
    13141 aagtaacgga gagttctgca tttttagcca ataattcaac ctggtttgta atctgcatta
    13201 ctaattcatg cgtaggtgca agtataagag cttgtgtttc tctttttgat gtatcaattt
    13261 tttcaaatat aggtaaaagg tatgctagtg tttttcctgt tccagtctgt gaattaatca
    13321 ataaatcctt attttgaatt atattcccta taacaagtga ctgaacttct gttggagaag
    13381 ttatgtcttg ttttttgagt ccatctatta gagtggaact tatttttaat tgttcaaatg
    13441 tattcatatt ttactcctta tattttaata attatagtca actatttttt attatacatt
    13501 gtttattcaa atcgaactat atattctttt ataattatgt gtaatttgaa agtaaatatc
    13561 aattatattt atacctttat atttctgata cctttgcatt ttatagattt ctaaaataaa
    13621 caaataaaag ttgtaattgt ttgatatttg ttatatatta tatataacaa atattaaaac
    13681 tataatatgg agggaaatta tgttaaaagt aatcaatgta tctaaatcat ataaaaataa
    13741 aaaagtagta agtagtgtct catttgatgt aaatgatgga gagctttttg ggtttatagg
    13801 acataatgga gcaggtaaaa ctactactat taaagcaatt gttggaatac acgattttga
    13861 agagggagag gtactaataa attctaaatc tataaaaaaa cagcctgttg aatgtaaaaa
    13921 ggaaatggca tatatacctg ataatccaga tttatacgag tcattaactg gtatacaata
    13981 ccttaatttt atagctgaca tatttgaagt tgccaaagat aaaagagaag agttaataaa
    14041 atattattca aataagtttg aactcaataa agcattagga gatttaatat cttcatattc
    14101 acatggtatg aaacaaaaat tagctataat ttcggctttg atacacagcc caaaaatctt
    14161 aattcttgat gaaccttttg taggtcttga cccaaaagct tcctttatat tgaaggaaat
    14221 tatgagggag ttttgtgaca aaggtggttg tatatttttc tcaacacacg ttttagaagt
    14281 tgctgaaaaa atttgtgata agattgccat aataaaagat ggaaatataa ttgcttatgg
    14341 aactacagaa gaagttaagg gaagtaattc acttgaaagt atctttatgg aattaataga
    14401 gaaataaaat gttttttata caatatagtg tctaaagtga ggaatgataa aatatgagca
    14461 atctaagtat attaataaaa actaatataa taaatgaatt taaattaaat gcgttaaaaa
    14521 aagctgatgg tagagaaaaa caaaaaataa tgggaatgat gctttcagtt gttataatag
    14581 cgggtctatt aattttttat gtaacaagct taagtctctc tttagttgat atcttaaagc
    14641 aaataaatca actggaaatg ctactgatat ttggttttgg attatcaaca ttaacaacta
    14701 ttgttacatc attatataaa acgtcatcat atctatttca agcaaaagat ttagaccttt
    14761 tgactagttt acctataaaa gaatctactg tactagcaag taagatatta atgttggttg
    14821 gagcaaacta tttattttct tcaatatgta tgattatacc tgcagtggtt tactttttaa
    14881 attctgatgt aagtataact tatttccctt atttgatatt gctattttta gctttaccat
    14941 tattaccgat agttgcttca tcaataattt ttctatttat agggcagata tcatcaagaa
    15001 taaaatacaa aaatttagta ttaatcatag gtagcattat attgatgttg ctttataccc
    15061 tacttatgtc aagacttaat tctatgtcag ttgaaatgct aaagaatagt gtgtctattt
    15121 cagagacaat tagaaaagta tatcctccta tatattattt tgttgatgct cttaaaacag
    15181 gaagtatagt ttcattttta gcatttatag ccatctcaat aataccattt tttatgtttg
    15241 taactctatt tgcaaaaaaa tttaaagata taaatgctaa gatgagtgaa ggatataagt
    15301 caaaaaatta tgaatttaag ggtcaaaaaa cttcaagacc ttcaaaagct ttattaaaaa
    15361 aagaatttaa gagatatact tcttcgttta tatacttttt aaattcatct gcaggtttgt
    15421 tatttctaat ggtaggtaca attttaatat tattatttgg agcagataaa gtgggtgagt
    15481 tactgaaatt gaatatagat tttaatctta taaaaataca attattggga gtattcttct
    15541 ttataatagt aatgaattgt acaacatact gttcaatttc tcttgaagga aaaaacttat
    15601 ggatactgaa atcttctcca atagatgaaa gagaaatatt ctggtcaaaa atattgatga
    15661 attttttatt gaatgcacca ctatctattt tatgtctaat attagtatct ataagattag
    15721 actttgaaat tcagtttatt attgtatcaa tctgtttgat tgtaagtttt tcaatgtttg
    15781 tagcattgat gggattatta tcaaatttat tatacccaaa ccttgattgg aaaaatgagg
    15841 tggctgtagt aaaaagaagt gcaagtatga tagttacaat gttagtatca ttagcttatg
    15901 taattatact tggaggtata tatgtattac ttggaatttc gtttttaaat atatatatta
    15961 ttttggcaag tatacttact ttgatattag attttatgat atggagaatt atttctacta
    16021 aaggagtaag aatatttaaa aatttatagg tgtatttatg tcaaataaag acttatcttt
    16081 tttagtcttg caaataatag gttaagtagt atactatgta tagtgatatg aaaaaattta
    16141 ggagaaaata aatgagaaaa ttatccaaaa gagaaaaaga ttttattcaa tatggatttt
    16201 tattattttt gatatgtatt actacttatt tagtattaac tactttagat ataaaactaa
    16261 tacctaaaat tataaggcta gtaaataaga cttatatatt tcttggcata ttgacaatta
    16321 tagtatatat gataattgaa tctattgtaa ctcatttgat aataaatgca gttcaaaaaa
    16381 caaaagtaaa atttattggt attaaaatgg ctacaatggg attttactat aacttagtta
    16441 ctccatttgc atctgggagt caaccaatgc aaatatatgc acttacaaaa tatgatgtaa
    16501 gccttagtaa atcagtggct atagttacta ataaaactgt agtatttcaa agtacagtta
    16561 ctgtattttg tggaatttta attttgctaa ataagtcatt actgatggag caagtacatt
    16621 ctgtgaaagt cttgataact tttggaatga ttatgaatgt aagtatgttg ttaggtggac
    16681 tactaatagt atttagtcca aaacaagtaa aaatgatagc agaagttttg attaattttt
    16741 tatctaaatt taaaacattt aaatttttag attcaaaacg tgatagaata aatcattata
    16801 ttgatgatta taattattct ataaaaatat ttattaaaaa tgtaagaatc ttagttttaa
    16861 gtatattact tactgtgtta caattaatcg catatttcag tatagtttat tgtgtgtata
    16921 aagcctcaaa tttaagagga gtttcatatg tacatataat gacattacag gtctttttat
    16981 atatggctat atcaccaatt ccaacaccag gaaatgttgg agctaatgag gttacatttt
    17041 ttactatgtt ctccaatata attccaaaag aattattggg gtactctgta ctcttatata
    17101 gtgggtttgt atactatttt atattaatag tttcaggttt atttacattt ggaacgcatt
    17161 atacattaaa ttcttggaaa aaggaaaata aagtaaattc taatttagat aaggttattg
    17221 aaaaattaaa ttaataaaat aattagggaa attctatata taaatatggt gttgaataag
    17281 ctatattata ttgagatatt tccctttttt gattaaaatt atagaaattt gaaataataa
    17341 gtaataaaca gaaaaagtga tattgttttg aaaggagaaa ctatgaaaca agataaacaa
    17401 aatgaaaaac aaagtgagat ttctaagata aaacaaagca atgataataa acttgaaata
    17461 gctcaagaat tacatgtaag taaaaatata gggaatatgc ttggatatag aatagataag
    17521 aaaaaaatga atagaccaag ttaatgaaaa tgttgaaaat ggtacatttt aaaaaatata
    17581 tataatgaaa tgttttattg ttaaatttaa acttatttta atgggggaaa aagtcgaaaa
    17641 atatctaaaa agatatggat ataaaaaata ttaatatata tatactataa acttgacaaa
    17701 atcaaccttt taataactag caaaaaatag caaaattata taagcgttat ttaattgtca
    17761 aaaatgaaag ctaaattgca tgtcctaaaa aaaatgattg ataacattta tttgtgtgct
    17821 ataattatat agtaaaacaa taagtatatt gcataatgta tgcaaatcgg gaggagagac
    17881 ggacatggaa gcatctatac acacaattgc cagcaataat ttattgataa tttttgctat
    17941 tgtaagtata actggaataa tttgtagtaa gcttagtgaa ataataaagg ttccagatgt
    18001 agtgttatat ttacttgtag gtattttaat aggaccatca tttttaaaat ttatagatat
    18061 tcgtggattt caaatagaaa atcaactaat acttacattt ggttctgcat ttattttata
    18121 tttaggtgga aaagaaataa gtttaaaagt attgagaaat gtgaagatat ctgtattctt
    18181 attagctact ttaggagttg tgatatctgc ttttataatg caacaagtaa taggatttac
    18241 ttttgggata agtgcaatga ctgctttact agcaggaact ataatagcat caacagaccc
    18301 agccactctt gtacctatat ttaatcaggt aaagataaag gatagggtaa aacaaactgt
    18361 aataagtgaa tcagctttta atgatgcaac aggagctata ctaacatcag cagtgttggc
    18421 tgtaattttg tcaaataaat tttcattagg tgaaaatatt tatgaactgg ggacaatggt
    18481 tattgtcgga gttttagttg gtttaattac aggaatactg cttttgaaat tagttaatga
    18541 taaaccttat ggaatattta aagattttgc accaataata tctgttattt cagttgtagt
    18601 tgcatatgaa ttatctacaa aattaggtgg aagtggatat atgtcttgct ttattgtagg
    18661 tataataact ggtaacaaga aaaattttaa aatttggtta agtcaaaaat cttatgatgc
    18721 agatttttat gttgctgaaa ctttaggtac tatttgtcgt atggcaatat ttataatatt
    18781 gggtagccaa gttaatttgg ctcagttaag taaatatttt atgccatctg taattacagt
    18841 attagtatta atgtttatag caagaccact ttgcgtttta gtttgtacac ttgtagacag
    18901 ggaagcaaaa tggagtaaaa gtgaaatatt atttatgatg tgggtaaggg aaacaggcgt
    18961 aataccagca gcactttgtg gtattatttc agcaatgaaa gttcctggat acgaagtgat
    19021 ttcatctgta gtattcatga ctatactaat tacattagta atacaaggaa gtactacaaa
    19081 attagtagct aaaaaacttg gattattaga agaagaagtg ataaatataa gtgaaaaagt
    19141 atcaactttt taaagttttg ctttaaaata gtacctgtta atggctattt taatattgtc
    19201 taatgatgat tattgaaata gtcatttttt tatgtataag taatttacag aatttacata
    19261 ttagacatat ttattatcaa aatttgttga agctatgata taattagttg tatttaaaat
    19321 aagaatagca aataagggag gaaatttatg tttacggtta tggatattgt acagccagat
    19381 acagtagaag aagcgtactc aattttaaat aaaagaaaaa ctaaccaagt aattggtgga
    19441 agtgcttttt taagaatggg gaaaaaaaga ataggaacag gaatagaatt atctaaatta
    19501 aatttagatt atataaaaga gtatgaagat tacgttgaga ttggttctat gactactttt
    19561 agaacattag aaacaagttc cataataaaa aataattttg gaagaataat agaagattca
    19621 gttaaaaata taataggagt tcagtttaga aatgtagtaa cagttggtgc aacagtattt
    19681 tctaaatatg gtttttctga cttgatagta gcattacttt cattagatac ggaagtagaa
    19741 ttatataata ttgggagaat tagtcttgaa gagtttctaa atagagatta tgagaaagat
    19801 ttacttatta agatatacat aaagaagaca aataagaatg cttcttataa atcaattaga
    19861 aatgcaaaga gtgattatcc aattctaaat gtatctgtat caaaacatat ggaacaattt
    19921 aagttatgtg taggagcaag accgcagaaa gctacgattg caaaacaagc tagtgaattt
    19981 ctatctaaca atgaaatcaa tgaaattaat atagataaag cagtagaaat agcatcagaa
    20041 gaactaactt ttggttctaa catgagagca tctagggagt atagaaaagc tatgagtaaa
    20101 gtacttttaa aaagagctat aatggaggtt atttaatgtt agtaaagatt aatataaatg
    20161 ggaagaaaaa aagtgttgat atagaaccag aagaatatct ggttgatact ttaagaaaag
    20221 ttggtaattt aagtgtgaaa agaggttgtg acacaggttg ttgtggttta tgtacagttt
    20281 taattgacaa gaaacctaca ttatcttgtg caacactgac attgagagct ttagataaag
    20341 aaataactac aattgaaggt ttagaaaatg aagttaaaga atttagtgaa gttttggtaa
    20401 aagaaggagc tgagcaatgt ggtttctgtt ctcctggctt tatacttact gtacttgcaa
    20461 tgaaggatga gcttgaaaat cctacagatg atgaaataaa gcattatctt acaggaaatc
    20521 tatgtagatg tacagggtat atgggacagt taagagctat caaaacttat ctggaggtaa
    20581 aatagaacat gaatatagtt ggaaaaggaa tcaagaaaat agatggaatg agtatcacaa
    20641 caggaaagcc tgtatataca gatgatttat cagcaaaaga tgctttggtg gtaaagatac
    20701 ttagaagtcc tcacgcttat gcaaaaataa aagatataga tacatcaaag gcagaaatgg
    20761 ttgatggtgt tgaatgtgtt ttaacatata aagatgtacc aaaatctcga tttacacttg
    20821 ctggtcaaac atatccagag ccatctccat atgatagatt aattcttgaa gatgttgtaa
    20881 gatatgtagg tgacgaagta gctatagtgg cagctataga tgaaaaaact gcaattaaag
    20941 caatgaatct tatcaaagta aagtatgaag tacttacacc tgttttggat tttgaagagg
    21001 caataggaaa taaaactatg gtacatgatg aagatagaca ttgtaacttt gatataggta
    21061 tgcaaagaga aaaaaatata gtatcccatc atgattatac aaaaggtgat gtagaaaaag
    21121 tattaagtaa ttgtgatgta gtgattgaag aaacttatta tactcaagct caagcacatg
    21181 caatgatgga gacgtatagg gcatacaatt atttagacca tatgggaaga ttggttgtag
    21241 taagttcaac tcaaatacca tttcatgtaa aaagacattt atctcgtgca ctagaaatac
    21301 cagctagcaa aattagggtt ataaaaccta gaattggtgg cggatttggt ggtaaacaaa
    21361 ctgcatgtgt tgagattttt tctgcaattg ttactttaaa aacagggaaa ccagctaaaa
    21421 taatttatga tagaaaagaa acttttaatt gttcaacaag tagacatgca atgagattaa
    21481 aagttagact aggtgctaca aatgatggca taataaaggc aatagatata aaagcacttt
    21541 ctgatacagg agcatatgga gagcatgctt ctacaactat tggtttggtt ggagaaaaaa
    21601 ctttaccaat gtataataaa gttgaagcaa caagatttat tgcagatgtg gtatatacta
    21661 ataagatgcc agcaggagca cttagagggt atggagcatc acaaggatgt tttgctgttg
    21721 aatcaactat aaatatgctt gctaataaat taaaaatgga cccaacagat ttaaggttaa
    21781 aaaatgtggt aacagaaggt gaaactacat ttgcatatgg taaaacttta aatagtgtcg
    21841 atttaataaa atgtataaat aaaggcaaag aacttattgg atggaatgaa aaatatcctt
    21901 gtaaacatct tgataatgga aaaattagaa gtgtgggtat ggcaattact atgcaaggtt
    21961 ctggtataca aggtcttgat aagtcttcag ctgaaataaa attgaatgac tgtggagatt
    22021 atactcttat gataggttct actgatatgg gtacaggaag tgatacaatt ttagctcaaa
    22081 tggctgctga ggttcttgat acaagcattg aaaatataac agttatatca gctgatactg
    22141 acttagttcc atatgaccca ggttcatatg cttcaagtac tacttatgta acaggtatgg
    22201 cagtagttaa ggcagctcaa gacttaagag aaaaaataat ttctgctgga gcaactaaac
    22261 taggtgttga gaaggatgaa gtagaatttg atggtataaa attaactagt aaaaaaggta
    22321 gcactttatt attcgatata gcagttgatt ctacaattgg tccagatact aatcaattaa
    22381 taggtcttgc ttctcatggt agcccagtat ctcctccacc ttttatagca ggttttgcag
    22441 aaactgaaat tgacacagaa actggaaaag ttgatttagt aaattatgta gctgtagtag
    22501 attgtggaac ggttataaat aaaaatctag ctaagataca agttgaaggt ggaattgttc
    22561 aagggattgg aatggcatta tttgaagaag ttagatacac agaaaaagga agcatggaca
    22621 caaatacatt tatgcaatac aagattccag ctagatgtga tattggaaat gttaaagtag
    22681 attttgttga aacttatgaa ccaactggac catttggtgc taaatctgtt ggagaagttg
    22741 ttataaatac acctttacca gcaatacaag aagctgtgtt taatggaatt ggtgtaagaa
    22801 taaatacact ccctattaca cctgaaaaag tttttatgga aatgaataaa taattattta
    22861 tgaaaattta gactagtttt acatgttaaa caaccagtta tgtcatacct gttgaaagat
    22921 atttaataag tagttattat aatattatga attttactta taaaatacaa aattaaataa
    22981 ggggatgatg ttttggctgg taatctaaat aatatgagag cagtaaataa ttttagagga
    23041 gataagaaca ttttagaatg tttagtcagc tttgagggtc gttcaataag tcagagaaaa
    23101 gtaagggtat tttttaaaga aaaacaaaat caaatagaaa ttgattttgc agaagaggaa
    23161 atttctaaat tggttgaaaa tgttgtttta aatacatcat atcaagaaat gttatatgat
    23221 gaaatagaga aacaactgga aattgattgt ataggtactt ggatgatatt atctaaatta
    23281 aaagatggta gtagagttca ctgattttaa aataaattat tagagcctaa ttttaaggct
    23341 cttttttgct gtttttaatt aacaatagaa aatttatctt tagtttgtat ataattttaa
    23401 tatcatatat tctatatata taaaattgaa tttgtatgta ttatattgta taatataaaa
    23461 tgaattggga aaatagttcg ctaaaataat ttgtttttat aaaattactg tttaagttta
    23521 tagattttgt ataaaataaa gttatgttat taattttttt aaaaataaaa cttttactag
    23581 gagacagata tgagaaaaaa tagtattaat atagcactaa ctgcagcaat tacagcaatc
    23641 tatgcagttt taacattatc attgggattt ataagttatg gaccaataca atttagggta
    23701 gcagagatta tgatgctttt ggcattttta gataaaggat atatagtagg tcttacatta
    23761 ggttgttttt tagcaaatgt aataggacca tatggtgtac ctgatataat ttttggaaca
    23821 ttagccacat ttataagtgc aagtatgatt tatattacta gaaagttagg taaacaaaat
    23881 aaaagtactt tagttatagc ttctttatgg ccaactgtta taaattcgat tgtggttggt
    23941 ctaatgttaa atgtattctt tgggcttcca ttaattctat ctatgataca ggtaggtttt
    24001 ggagaatttg tagtagtaac aattgtagga gtaccatttt tctgcttttt aatgaaaaaa
    24061 tataaaaatg tcattaatat taaattttaa atagtgtata gtaatttaat taattgagag
    24121 tattattagt gaaaagaggt gtttatttgt cgaaggtaaa aagattgact gatgtttgga
    24181 tatatattaa tgataagcaa aagtttacaa taaaagagtt gtcagaggaa tttaatcttt
    24241 cagctaaaac aatacaacga tatttaaaag aattaaataa aatgggactt ccaattcaag
    24301 cagaacaagg tagaaatgga ggttataggg tgctaaataa tagctatata cctcctgtta
    24361 tatttacaga aaaagaagtt atgattatac tatttgcact taagtcactt cagatttata
    24421 attataagtt aatggaaata gaaattaatt ctataatgag aaaagtaagt tatgaacgta
    24481 aaagcagtat aaaagtaggt atagaaaata ttaaaagata cattggcttt gtagctaatg
    24541 atactaatga tggagatttt aagagtagta atgtaactga gttttttaga gcttcttcag
    24601 aatctctaat tttaaatatt aagtataagt gtggtaatca gattttggaa aaaaacgtat
    24661 gccctatagg tatatattta aataagggag catggttttc accagcatat gattgtaaaa
    24721 gtgataaaat aatattagtt catatagata atataattga tataaaaaga acaggagaaa
    24781 gtaaaaaaat acatataaac ttggaagaat ggttacatga agtatataac aatttatatg
    24841 gaaatagttc aactactaaa aaatttggtg aaattattta tctaaatgta ttattaacta
    24901 aagaaggtgt ttctaaaata aaggacagtt cttggaattt agatatggag ctaaatgaag
    24961 atggtagtgg aataattagt acattcataa aggttgaaga tatagattgt tatgcttcga
    25021 ttttattttt tattggaaat aatgctaagg ttatagagcc aaaatttttg aataagctac
    25081 tttatgataa ggccaatgag ttaagatatt tttatgaaga aaatatggta taatatttag
    25141 aaatgttgaa attaaataat tattaaggag atatacgtta taatggaaaa ttttaataag
    25201 aatgctaaaa taattggtat tggagctgaa ggtattaata taataaatga agtagaagaa
    25261 aaaataaaag caaatatgga tatagaaaaa ataaacataa atcaagagat tgaaaaagaa
    25321 tatgttagaa gtcttttaga tggtgtagat atattatttt tgatatattc atctgaagat
    25381 aaacatatta gagatattat aaaagctgtt tcatatatgt caaacgaaag aagagttcta
    25441 tctataggaa tggattgttc agagaaggaa aataaagaag atttagagct tgggagagag
    25501 tttaaaatca ataatgatag tatatttaaa tttgttgatt taatgaatat catggtagag
    25561 tctatttctg attcttgcat gataaatata gatattactg atttaaaaga agctattgta
    25621 ggagataaag gaattaagta ttcttttgaa gagtttgaag atacaaagtc atatagcgag
    25681 atagctgaca ttttatttga caggatggaa tatattggtg aggaatttat aagtaagaaa
    25741 ggaattgtat ttgttgaagg aagtccagaa ttttctataa tggaattgaa tgatttaata
    25801 agtaatattc agagcaaggt agaagaaagc tatgaagtaa tattttcttt atatataaag
    25861 gaaaatttaa atggaaatat tagagtaggc ttattgtata attagtatat tttagatatt
    25921 aaggagagtg tatatgtctg aaaatataag aggtacagag tattggttaa tggaagaatt
    25981 tttttatcag gaaacagaca caccacagat ttttagagaa gaaagcataa ttgcacttgg
    26041 atttgatata aaaataaatt taaaaaaagt attcagtatg gggaaaaaat cagaaaagta
    26101 tattaatgag aattgtaatg ataaagattt tataaaaaag tttatgtcga ttaaaaaagg
    26161 agattatttt ttatataaag catatcaaaa tatagaagaa aaagttaaat gtaatgtgtc
    26221 tattggcata gttgaagaag attttgaaaa tggatatgaa ttaagtcata ttttagggca
    26281 tacactacca gtaaaatgga tgcataattt tgatacagtt tcaaattgta aagtatatac
    26341 aagtgaattt tataaattaa ataataatga tattagttca tatttagaaa ttattaagtc
    26401 acaagataag aaattattga gaaaaagaat agaagaaagt gaaaataata aatggtattt
    26461 aaaaaactat tattataaaa atgtagataa gtgtgtagat gatgaagaat tatttattca
    26521 gcccattaat ctagaacaat tagaaagcct aaaaaatata aagacagatg attatatttt
    26581 attgtataat agcgagaaag taatttgtga aaatgaccat acaatgaaga gtctatttgg
    26641 cgtatgtcaa gttatggaag ggttcgataa agagaaagta agtgctttgg agggtggata
    26701 tttttttcct gttagatgga tagagtatga aaaggagaat atagacggaa atgcatgggt
    26761 tatatttgaa aataatagta tagttgaaaa gtatgtaaaa agatttacta ataaaatgcc
    26821 attaaatact atactttatg gaccaccagg aactggtaaa acatacaata caaaacagat
    26881 ggtttacaat ctagtaagtg aaaccagtct aagaaacaga tactatacag gttacttaga
    26941 ttataaaaat aatgtagaat tttgtacatt tcatcaatca tatggatatg aggaatttat
    27001 agagggcctt aagtctgatg gtgaaggcaa ttttaagcca gaagatggga tattaaagga
    27061 tatatctata gaagcatgtt ataatgcatt gaaatttgaa aaaaagatga gctttgagtt
    27121 ggaaaaagat agcttgactt tagaaactat aaaagacagg aaaaagaaat tagtattaga
    27181 aaatatagaa tatagtgaga gttttaattt tgaagaatca gaaaactatg ttttaataat
    27241 agatgaaatt aatagaggga atgtatctaa aatatttggt gagctaataa cattattgga
    27301 agatgataaa agacttacta aatcaaatca aactattgtc aaactaccat attcaaagga
    27361 aaaattttct ctacctccaa atttgtatat agttggaact atgaacacat cagataaatc
    27421 aatagcactt ttagatatag ctttaagaag aaggtttaat tttgaagaaa ttatgccaga
    27481 ttatgattta ttgtctattg tagatggtat agatattaga aaaatgctat atacaatcaa
    27541 taaaaggata gagtttttat atgatagaga ccatgttatt ggacaagctt atctacttgc
    27601 tgtagaaaat attaatgata tagtagtagt atttagaagt aaaataatac cacttcttca
    27661 agagtatttt tattatgatt cagaaaaagt aggtttgatt ttaggaggaa ttggaagaaa
    27721 tgaaaaagat aaatacattg tttataaaga aaaacttata gcttctgagt tatttaaaaa
    27781 taataatgcc tcaataatgg agagtaaagt taactattgt ataaaacaaa acatatctag
    27841 tgaggagcta aaaaatatat atgagtaaaa atatatatgt taaggaaacc tatgaatgga
    27901 taaggatagg taatggtgaa aatgagctta cagaaattga atatgaaaaa ttgcttaagt
    27961 atctagagaa caataatgat gtattaaaaa gtaatataat agatattaaa tacaaaaaac
    28021 ttagatttat aaactatgta ggcatcatat gttttgaaaa tgtaatactt gaaatacttc
    28081 ctaaattgtc attaagtgat aatcttgtaa aagatagaga gatactatta cagatgcttt
    28141 ctatatgtaa taaaattcca attacaatga atgaaaaaat aagattgagt ttgaaaaatt
    28201 ataatctctt aaattttttt gttatgtatt tcattgagag tatgcaaaca cagatgaaaa
    28261 aaggtattta ttttgaatat ataaataaaa tagagaactt aaatgttatg aggggtaaaa
    28321 tattattaag tacatatgca aaagaaaaag gcatttctcc aatgaaaata agatgtgaat
    28381 atgatgagta tagtgaaaat aatttcttga atcaagttct taaaaaagca tgtatatcta
    28441 ttttatgtag aataaatgac aattcaattc aaggcaagat taaaaaaatt ttaagctatt
    28501 ttcaaaatgt agatttaatt tatatagata gaaaaaaact gttggattat aaattttata
    28561 aaaacaatga tagatttaaa gattgttatt tactagctag acttctactt ttaaatttgt
    28621 ctatggataa tagtcaaaat aatcaagagg cattttctat attatttgaa ataaatactt
    28681 tgtatgaaga atatatagga attttaatca aaagtatatg ggacaattca tttagagaaa
    28741 catatataca agataagagt aaatttcttt tgaaaaatga acaaactggt aagaaaaact
    28801 ttaatttaag accggatata gttttatatg acttaaaaaa tgaatatgaa attataattg
    28861 acactaaatg gaaagctatt gaagtagatt caaatgtttt ttataggtca agtgatatat
    28921 accagatgta tgcatatata acagcatatg aaaatgcaaa aagatgtata ttattatatc
    28981 cttgtattca aaaagataag aattatagtt cctggaaatt atcagaatca tttaagggta
    29041 agtttataga agcaaaaact gtgaggttag atgatataaa aaacacaaaa aatgatttaa
    29101 aaaagataat ttttaattat aaattttaaa aataagaatt ttaaaattta taaaagtaat
    29161 tttatatagg tataaaaatt acgagtagta taaaaaattt aataagaact taaattaaaa
    29221 attttaaaaa ttatttccat ataaaatatg gcattgtaat tgcttattta atagaggatt
    29281 tttgtaaaat gaagaaagta tatctatcaa attaaaacta agtagatata ctttttatta
    29341 agttttgttt taaaataata ttattaaatt ataactaagc ttttctgtta tgatataatt
    29401 aaatagataa tgtgaatgat tattatatag aatacttatt tggagttgat attttatgga
    29461 caaacacagt gattcagtaa tgtatggtgg aatggaagta tataatattt ggggaacaac
    29521 aacagtagac tccataattg gagagtctga tgtaatgcgt gctcttaaga atagaatttg
    29581 taaaattgga aatagtgatt ctactgtagc tataactgga gaaagtggca ctggaaaaga
    29641 attaatagca agagccattc atctcgcagg aaacagaggg aataaaacat tcgttgctat
    29701 aaactgtgct gctataccag aacctttact agaaagtgaa ctttttggat atacaaaagg
    29761 agcttttagt ggagcagatt caaaaggtaa aattggtaag tttgaattgg ccaatggagg
    29821 ggttgtattt ttagatgaga taggagatat gccaattcaa ctacaatcaa agttattgag
    29881 ggtattacag gaaaagaagt ttacgagaat aggttcaaat gaactaattg atatagatgt
    29941 tagaatcata tcagctacta atagagattt atttgagttg gttaaggaaa ataaatttag
    30001 agaagactta tactatagat tgaatgtaat acctttagaa gtgcctccac ttagagaaag
    30061 aggagacgat atacaaatat taataaacaa ttttatagac aaatattcga gaaagatgaa
    30121 gaaaaacgtg taccatatag agccagatgt ggaacaaatg ttgcttaagt atccttggcc
    30181 aggaaatatt agagagttgg aaaatactat agaactggca ttaaaccttc ttgaggaaga
    30241 tggaataata cataaaggaa taataaatag aaagatactt gaatatttta agttaaataa
    30301 tataaatata aagttacttg aggaaagtga ctatgagata agtatggaac aagacatact
    30361 tacacttgaa gagtttgaaa gagcctatat aaagaaagtg ttaaagtttt acggaaatga
    30421 tactaagtct aaaaaactag ctgccaaaaa acttggaata tcactagcta cattgtacag
    30481 aaaaatagat gtataataat taggcttttt attctcaatt ctaaaatttg aaattataaa
    30541 attgataaaa aacacttgga aaactatttt ctaagtgttt ttttgtgtaa tagcatttta
    30601 ttatccaaaa ataaaaaaaa tttaaaagaa tttaaaaaaa taaatgcttt aataaattat
    30661 caaatactaa caaatataat aaaaattata tatgtaaaac aattatgtta aaaaattggc
    30721 attataattg cttcatatta agttgagatg acaaaataaa aaaagaaagg atgttgtgtg
    30781 agataataat aactgtcatt aaaaaatagt tgggagtgat aaccttgagt aaatatcaaa
    30841 agaatgtttc tatgtttgat attgatggaa ggccaccaat tttaaagtgt ttgccactat
    30901 caatgcaaca tatactagca atgatagttg gaactgttac agtacctata gtcgtaggag
    30961 gagctgtggg tgttagccct acacaaataa cacttttagt gcaatatgca ttaataatag
    31021 ctggtatatc aacacttata caactatatc caataggaaa tattggttca agattgccta
    31081 taatttttgg agttggtttt acttatgtac caacacttgt agcagtcgga ggaaattatg
    31141 gattagcaag cattttgggt gcacaattaa tagggggagt ggctacagta ttaataggga
    31201 tattcataaa aaagataaga aaattttttc cgaatatagt tgcaggaaca gtggttttta
    31261 caattggatt atctttgtat cctatagcaa taaattatat ggcaggtggt gtaaatagtc
    31321 ctacttatgc ctctatgcaa aactgggcag tagcggctat aacattagtg gtagttatag
    31381 gtcttagtca atttgcaaaa ggatatgcaa aattggcagc aatatttttg ggaattatag
    31441 ttggatattg tgtatcttta gttctgggta taataaattt tgaccctatt agagaggcaa
    31501 cttggtttgc gatacctaaa ccgcttgaat ttgggatgga atttgatatg cctgtaataa
    31561 tctcagtagt aattgtaagt ataatcaatg ctgttcaagc aattggagat ttatcagcta
    31621 ctacaatggg aggcatggat aggaatatca cagataagga attatcaagt ggagtaatag
    31681 gaagtggaat ttgtactatg ataggagctt tgtttggagg tttaccacca tcatcatata
    31741 gccaaaacgt ggggatggta gctatgaaca aagtaataag tagatttgtg cttggaatag
    31801 ctggtatatt tatgttattg tcggggtttg tgcctaaatt tggtgcacta atgactacta
    31861 taccatattc tgttttagga ggagctacaa tatctatatt tagtatgata acaatgacag
    31921 gtataaagtt aattattaca gaagagttgt catcaagaaa tattaccatt gttggtttgg
    31981 caattgcctt aggcatggga ataacatcag taccagcagc ccaagaggca tttccttcat
    32041 ttgtaaagtt agtatttgga gaatctccaa tagtaattgc aacattggtt gcattcgttt
    32101 taaatctaat aataccaaat aaaagtctag aggatgaggc taaagaaaga gctgttatgg
    32161 aagagactaa ttaagatata ataatttaaa agaattgttt aaattattta gtatgagatt
    32221 gttagattat ttttgatagc tattcatttt aataaaaaat ttaacaaata aatatatacc
    32281 aggcaattaa tgttcattta agttgaaaat cggggaggga atatttagaa atttgttaat
    32341 tttttataaa aatagctcag tttagctgtg tttatcggtt gagcaataaa ttcatggtca
    32401 taaaaatatt tatagaaaat aattataaaa atatttagtc aagatattta tgtatgggag
    32461 gatttatttt atgaaaacac gtaatacttc aaaatatgat gtcgatggga taccaccact
    32521 aaaggaatca atacctttgg ccttacaaca tctgttagca atgatagtag gaaatatggt
    32581 acctgcgata ttaatagcaa acgtggtagg acttaaccag gggcaagcaa caatgttgat
    32641 tcagggttct atgttagcag caggtttagc aacgttttta cagttatatc caataccttt
    32701 atttaaagga tttaagttag gttcaagatt acctgtaatg atgggaatga gttatgtatt
    32761 tttaggagct tgtctttcag tagcagctaa agatggttta gcagctctat ttggagcaca
    32821 gattgctgct ggggtaatcg tattctttgt aggatttgga gttaagaaaa ttagacatat
    32881 attcactcca atagtttctg gaactataat agcatgtatg ggacttggac tattcgcaac
    32941 agctataaag aatttagctg gaggagaagg cacacaaaca tttggaagcc caataaactt
    33001 cacagtagga gttatagttg cttttgtaat aataatgata aataaatacg gtaaaggttt
    33061 agtaaaaaat tcttcaatat taataggaat attagtaggt tatgctatat ctttagtttt
    33121 aggattggtt gatttctcag ccgttcaagg agcagctata gtatctttac caacaccagc
    33181 agcttttgga ttagaattta gaccagagtt aatagtaatg tttactatta tttacataat
    33241 aggtatagct gatatgatgg gtgcctgtac aatagctaca gtaggagcaa tggatagaga
    33301 ggtaactgac gaagaattag catcagttgt tttaggaaat tctataactt ctataatttc
    33361 atcactattt gcagctcttc caactggagt atttagccaa aatacagtaa ttgtatctat
    33421 gaacaaagtt acaagtagat ttgtaatagc actaggggca cttgtattat tactagcagg
    33481 gatatctcca gcattgggtg caataatgac aactatacca tcttgtgttg ttggtggagc
    33541 aactttagta gttttctcat caatagcaat gtctggtttc tctattatga gtatggatgg
    33601 attcactgaa gaaaacaatc ttatagcagg tgtatctata gcaacaagta tgggtcttac
    33661 aactgctcca caagttttag accaattccc agaaacaatt ggaactgtcc ttggagggtc
    33721 aagtatagtt tctggagcaa taatagctat attacttcaa acattattta aattaaagtc
    33781 acgtaaaagt gctgaaaacg ttacttcaaa tcttgaggaa aatattggat aaatgtatta
    33841 gatataatat gagaaaacca tatggtaaaa tgttgtaatg aaagtattca aagggggatt
    33901 taagatgatt gatatactaa taaaaaatgc aataattgtt acagttaata aagacagaga
    33961 tgttatattt gatggagcaa tagctattca aggtgacaga atcttggaca taggaaatac
    34021 agttgatatt gagcctaagt atcttgatgc taaaaaagtt atagatgcta atggaaaagt
    34081 aatattccct ggctttataa atactcataa tcacctgttc caagtgttat taaaaggttt
    34141 aggtgatgat atggcacttc atgagtggtt aaatacaatg atgtttccat cagcaaaatt
    34201 cttaacagaa caagatactt atgatgctgc aatgcttgga tgtatggaag gtttaaaaag
    34261 tggtataact acaatggttg attatatgca tactcacaat agaccaggac ttacagatgg
    34321 aatagtaaag gcatataaag atttaggtat aagaggggtt gtaggtagag gttgtataga
    34381 tttaggtatt cacaaagagc ttatagaaga tgtggaaacg gttgaaaaag atttgagaag
    34441 ggtattcgaa aaatatcata attcagaaaa tggaagaata aaaatctgtg tagctccttc
    34501 ttcaatgtgg gctatatctg aagaaatggg taagatgctt tggaatatag ttaaagaata
    34561 tgattcatat tttacagttc atatatctga aacagaattt gcaagaacag caacaaagga
    34621 tatacatgga gaaatagata taaaattact tgaaaaatgg ggtatagtag gtccagaagt
    34681 tgtagcagta cattgtgtat gtataactga tgaagacatg gaaatgctta aaaagtatga
    34741 cataaaagtt tctcataatg tagcaagtaa catgtatctt gcttctggtg ttgcacctgt
    34801 gccagaaatg ttaaaaaaag gtataaatgt gagtttaggt cttgacggtg cagcaagtaa
    34861 taacgcacaa gatatggtag agcttatgaa attaacagct ttacaacaca aagtaaataa
    34921 ttgtgaccct ctggcaattt ctgcagagaa agttcttgaa atggctacaa ttgagggagc
    34981 aagaactctt agaatggaag atgaaatagg ttctttggag attgggaaaa aagctgacct
    35041 agtaatattt aactcaatgc tttctccaaa atcaatacct cttcacaatc ctgtatcaac
    35101 tttggtatac tcagctagta tgcacaatgt agagagtgtt ttagttgatg gaaatgtaat
    35161 acttgaaaat ggaaaagtaa ctacaattga aagtgaagaa caagtgttgc ttaatgcaca
    35221 acatgcagca gaagaattat gtaagagagc taatgtaact aatagaatgg aaggacataa
    35281 gtggaatagc ttatactaat attaaaataa gattgtaata atattctatt acaacattag
    35341 tttatctgta ggaggagaaa atggaaaaaa taaaattaaa tattaatgat tacatgtatg
    35401 aaatagaagt tgaaaagaat gaaactcttt tgcatgtact tcgtgaaaaa ttaaatctaa
    35461 ctggagcgaa acaaggttgt aatagtggca gttgtggagc atgtaaagtt ttgatagatg
    35521 gagttgacat gaaatcttgt aaacttcttg caaaaaactg cgaaggtaaa gttatataca
    35581 ctatagaaag ttttgctaat ggagaaaatc ttcatccaat acaagaagct tttatagaag
    35641 tcggagctgt acaatgtgga tattgtactc ctggtatgat tataacaaca caagctcttt
    35701 taaatagaaa tgcatctcct aatgaagatg aaattagaga agcttttaaa gaaaatctct
    35761 gtagatgcac aggatatgta aagattatag aagctgtaaa attatctgca gctagattag
    35821 ggggtagata attatggaaa aggtaatagg aaaatcatac cctctaaaag atgcagcttt
    35881 aaaagttact ggtcaaatga aatacgtggc tgatatgaaa ccacaaaatg tactttacgc
    35941 taagatgctt ttaagccctg tagcacatgc aaaaataaaa agtatagata caagtgaagc
    36001 tgagaaagta gtaggtgtta gagctatagc tacttatctc aatacatcac aaagaaaata
    36061 caatggttca ttaagatttt atgagcacaa aatacctgaa aatgaagtta tattttcaga
    36121 tacagttaga tttgttggtg accgtgttgc agcagtagct gcagagactc cagaaatagc
    36181 agagaaagct gtaaaactta taaaagttga atatgaagaa ttgccagctg tatttactat
    36241 acaagatgca ataaaagaag atgctatctg catacatgga gattcaaata tagtttctca
    36301 aaacaacata aatgggggag atgtagaaaa aggcttcaag gaatgtgatt atatatttga
    36361 agatgaatac tctactccag caatacatca tagtgcaata gagactcata gtgtaattgc
    36421 acactatgat tataatgata aattaactgt acatacacct tgccaaaata catttggatt
    36481 tagaataata ctatctcaaa tttttgggat gtcattaaat aaagtaagag ttataagacc
    36541 tgctattgga ggctcatttg gtggaaaatt agaacttaca attgaaccag tagcagccgc
    36601 attatcaaaa atgacaagaa gaccagttaa aatggttctt acaagaaagg aatctatgat
    36661 atcaacaaga actcgtcatg ggtcttttgt aaaattaaaa actggtgtaa ataaagatgg
    36721 tgaagtaata gcacaagaca taaaaatata tacaaataca ggagcatatg cattaagtgc
    36781 tttaaatgtt ataggagcac ttagtcataa agtatttaaa gtttataaaa taccaaatat
    36841 aaagtttact gggatgcctg tatatacaaa tacaccaata gcaggagcta tgagaggcta
    36901 tgggtcacct caaatattta tggctcaaca agctcaattt gcgaaaatag caaaggaaat
    36961 aggtatggat ttagtcgatt ttcaaaataa aaatgcagta gagccagatg atgtcgatat
    37021 aatcttccac ggttctcttg gaaatcctag agtattggat tgtatagagc aaggtaagaa
    37081 gatgtttaag tgggatgaaa agaaaaaact tccaaaagaa gaaggaaaat atttaagagg
    37141 tataggtatg gcaataggtg cacatggtaa tggagttttt ggtgcacata gagatgttgt
    37201 tacattgact cttaaattaa atgaagatgg tacgttgaca ctactaacag gagctcatga
    37261 tatgggtaat ggtgttgtaa ctatgcaaac aatgatggta gctgaagttt taggaataac
    37321 acctgataaa gttgatacat ttgaaactga tacggatgca tgtacattca accttggtga
    37381 ctatgcaagt agaggggtat ttgtagaagg tggaggagct aaaaagacag cagagaaatt
    37441 gaaaaatctt atattagaag aagcagaaaa attacttgaa acatcaaaag aagatttata
    37501 tctagacaat ggatgtgtaa ttagtaaact tgatgaaaat gtaaaagcat cactttctga
    37561 tgtcgcagta tatagtcaaa gtaaatcatt aagagagtta actgttgttg aagattattc
    37621 ttcaccagct ggacgtactt cttatggtgt acattttgct gaagttttag tagataaaga
    37681 aactaaggat ataaaagtag ttgattttgt agctgtacat gatgttggta gagttataaa
    37741 tcctctaaat ttagaaggac aattagaagg tgggattcat atgggtctag gatacgcatt
    37801 gagtgaagaa ttggcatttg atgaacaagg tagacctaaa gctactgatt ttaaaacata
    37861 taaattattt agaactgtag atatgccaaa atgtcaagtt gcatttgtag atggttatga
    37921 aaaagctggg ccttttggtg gtaagagtat tggagaatgt gccgttgtac cagttgcgcc
    37981 agctgttgca aatgcagtat ataatgcaac tgatgtagag atacattcat taccaataaa
    38041 attaaaatag tagaaattaa agcagaaata acaatttatt gtatttgtaa atagataata
    38101 aaaatagatt tatttggtga ggagatatta ttatggcaaa gatattaggt atttgtggaa
    38161 gtccacgtaa aggagcaaca gaatatgcag tgttagaagc tttgaaagaa gcagaaacta
    38221 tacctggtat agaaactgag tattggtcag ttagagggaa aaaaataagt ccttgtgtac
    38281 attgtgatgc atgtataaga aaagaaacta tgtgtattat aaaagatgat atccaagaat
    38341 tagagcaaaa aatattggag gcagatggaa ttatagtagg ttctccagtt tatgatatga
    38401 atataactgc tcaacttaca gcagtattta atagacttcg tccaatgtat ttagttcatc
    38461 caggaaaact tcaaaataag gttggtgcag ctataactac tggtggaact agatatggtg
    38521 gccaagaatt gacaaaactt cctataataa atttcttcct aatgcatgaa atgttagtgt
    38581 ctggaggttt aggtggatgc tatataggtg gtacaatatg gagtaaagat ggaaaagcaa
    38641 aaggtgccca agaggatgag acaggtatgg acacagtaaa aagacttgga aaaggtgcag
    38701 cagaggcagt tatggtttct aagtttggtc ttgaaaaatg gaatgtcgta aaagaagaat
    38761 tagatgttaa aaaagatgaa aaatcacctc ttagagacca ctaaagattt attatattat
    38821 tgaatatata caaatagtga tgtaatagat tattgcatca ctattttatg ttttaattta
    38881 gattttataa attttatatt aaaaaatata gaaacaaagt gttttgagat ttatttttaa
    38941 attttaaaaa tgagaattaa aaagtgagaa gatagaaaag gttttttagt tattaaattc
    39001 tacatacatt gtatgaacta aagatagaaa agaaaaaata ataaaagcat tgcaatttct
    39061 atactaaaga gtcatggaaa aatatctagt atatatttag tattggcatg ttaattgctt
    39121 atatattaaa gtataaaaat ttaacaataa tttggaggta ttttagatgt taaaagaaat
    39181 taaatggaaa gtaaataatt tgccaaaggg cgataaggaa aattgtatca aatttttaaa
    39241 tgaagaagaa ataactaagg ttagaaattt tcacaaaagc tttcctcaat ataaagaaac
    39301 tccattagca aacctagaag gtttagcaaa aaaattaggt gttgcaggag tatatgtaaa
    39361 agatgaatct tatagatttg gattaaatgc atttaaagta ttaggtggtt cttattctat
    39421 gggtagatac ttagctcaaa gattagatac agatatatct gagttagggt atgacaaatt
    39481 gacttcgaaa gaaataaaag aaaaattagg agaaataact ttctttacag ctacagatgg
    39541 aaatcacggt agaggagttg cttggacagc taataaatta ggtcaaaaat ctgttgtatt
    39601 aatgccaaaa ggttcttctg aatttagatt aaataaaatt aaaggagaag gtgcagatgc
    39661 tagtataaca gacttaaatt atgatgatgc agtaagatta gctaatgatt atgcagaagc
    39721 tgatgaccat ggagtaatgg ttcaagatac agcatgggac ggatatgaag aaataccagc
    39781 atggataatg caaggatatg gaactatggc tcaagaagct atagagcaat taaaagaata
    39841 tggagtagat agaccaacac acgtatttgt tcaagcagga gttggttcac ttgctggtgc
    39901 agttcaaggg tatgttgctt caatatatga tgaatgccca ataacagtag tagtagaggc
    39961 tgatgaagct gattgttact ataaatcagc agaagctgga gatggaaaac caagatttgt
    40021 tggaggagat atgccaacta taatggcagg gcttgcttgt ggagagccaa atactatagg
    40081 atttgaagta ttaaaaaatc atgcagcagc atttgtttca gcaccagact gggtttctgc
    40141 taaaggtatg agaacattag gtaatccttt aaatggtgat gaaaaggtaa tttctggaga
    40201 atctggagca gttacaactg gtcttttagt agcagctatg gaaagagaag atttagcaga
    40261 tttaagaaaa gatttaaaat tagatgaaaa ttcaagaata cttttaataa gcacagaagg
    40321 agatactgac cctgacaaat atagaagtat agtttgggat ggagaatatc caagtatata
    40381 aggaattact aaaatgcaat tattagatat tggtttacat atttagaaga tatataagtt
    40441 ctaaaattta gaaaatatat aagtatataa aatacataca ttataaaaaa tctaggggga
    40501 taatgaaatg ttaaacgaac aaagaaaaca agaagtaact gaaatatgcc aaaaattaat
    40561 tcaaaatcct agttcttctg gaaatgaaga aggtgttgta aaagcaatag aagagtcttt
    40621 tgaaaaatta ggttttgata gttggtcaag agatagatat ggaaatatag taggatgtat
    40681 aaaaggtaac aagccaggta agaaaatatt atttgatgga catatagata cagttccagt
    40741 tccagatgca agtaaatgga gtgttcctcc ttttgaagga aaaatagttg atggaaagat
    40801 atatggtaga ggaacttctg acatgaaagg tcaatatact gcaatgatgt ctgctgtagc
    40861 gtattttgct aaagacacta atagagattt tgctggagaa ctatatgtag caggtgttgt
    40921 tcatgaagaa atatttgaag gtgtttcggc tagagagata agtaaggctg ttcaacctga
    40981 ctatgttgta ataggtgagt cttcagagtt aaacttaaag attggtcaga gaggtagagg
    41041 agaaatagta gtagaaactt ttggtaaacc agctcactct gctaatccag aaaaaggt
//
LOCUS       DAYAOY010000029        39800 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYAOY010000029 DAYAOY010000000
VERSION     DAYAOY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.029"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            260..1444
                     /locus_tag="ACGI6B_001594"
     CDS             260..1444
                     /locus_tag="ACGI6B_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751041.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1821..2948)
                     /locus_tag="ACGI6B_001595"
     CDS             complement(1821..2948)
                     /locus_tag="ACGI6B_001595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9751042.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3393..4586
                     /gene="nifV"
                     /locus_tag="ACGI6B_001596"
     CDS             3393..4586
                     /gene="nifV"
                     /locus_tag="ACGI6B_001596"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HFG9751043.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4611..6536
                     /locus_tag="ACGI6B_001597"
     CDS             4611..6536
                     /locus_tag="ACGI6B_001597"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HFG9751044.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6558..7553
                     /locus_tag="ACGI6B_001598"
     CDS             6558..7553
                     /locus_tag="ACGI6B_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HFG9751045.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7878..8351
                     /locus_tag="ACGI6B_001599"
     CDS             7878..8351
                     /locus_tag="ACGI6B_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9751046.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8385..8879
                     /locus_tag="ACGI6B_001600"
     CDS             8385..8879
                     /locus_tag="ACGI6B_001600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9751047.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8958..9497
                     /locus_tag="ACGI6B_001601"
     CDS             8958..9497
                     /locus_tag="ACGI6B_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9751048.1"
                     /translation="MKGMIYMIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSK
                     GLRTWEFIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAA
                     SYIQLQAHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEE
                     KKAYGDSDLDYKKVHFNNF"
     gene            9686..9997
                     /locus_tag="ACGI6B_001602"
     CDS             9686..9997
                     /locus_tag="ACGI6B_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9751049.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10025..10711
                     /locus_tag="ACGI6B_001603"
     CDS             10025..10711
                     /locus_tag="ACGI6B_001603"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFG9751050.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            10943..11827
                     /locus_tag="ACGI6B_001604"
     CDS             10943..11827
                     /locus_tag="ACGI6B_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFG9751051.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            11926..13101
                     /locus_tag="ACGI6B_001605"
     CDS             11926..13101
                     /locus_tag="ACGI6B_001605"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HFG9751052.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            13229..14083
                     /locus_tag="ACGI6B_001606"
     CDS             13229..14083
                     /locus_tag="ACGI6B_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751053.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            14127..15362
                     /locus_tag="ACGI6B_001607"
     CDS             14127..15362
                     /locus_tag="ACGI6B_001607"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFG9751054.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            15463..16404
                     /locus_tag="ACGI6B_001608"
     CDS             15463..16404
                     /locus_tag="ACGI6B_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HFG9751055.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(16623..16976)
                     /locus_tag="ACGI6B_001609"
     CDS             complement(16623..16976)
                     /locus_tag="ACGI6B_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9751056.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            17272..18183
                     /locus_tag="ACGI6B_001610"
     CDS             17272..18183
                     /locus_tag="ACGI6B_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HFG9751057.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            18413..18877
                     /locus_tag="ACGI6B_001611"
     CDS             18413..18877
                     /locus_tag="ACGI6B_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HFG9751058.1"
                     /translation="MEKIKKENLKMISPIILSSINQNLKEIERNELLETNIESGDYGL
                     ALSESDVKDIINSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQE
                     IFYYLKNETDDKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            18900..20435
                     /locus_tag="ACGI6B_001612"
     CDS             18900..20435
                     /locus_tag="ACGI6B_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HFG9751059.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            20519..21886
                     /locus_tag="ACGI6B_001613"
     CDS             20519..21886
                     /locus_tag="ACGI6B_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9751060.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            21921..22145
                     /locus_tag="ACGI6B_001614"
     CDS             21921..22145
                     /locus_tag="ACGI6B_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HFG9751061.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(22595..22993)
                     /locus_tag="ACGI6B_001615"
     CDS             complement(22595..22993)
                     /locus_tag="ACGI6B_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HFG9751062.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(22996..23439)
                     /locus_tag="ACGI6B_001616"
     CDS             complement(22996..23439)
                     /locus_tag="ACGI6B_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9751063.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            23757..24743
                     /locus_tag="ACGI6B_001617"
     CDS             23757..24743
                     /locus_tag="ACGI6B_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751064.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            24727..25638
                     /locus_tag="ACGI6B_001618"
     CDS             24727..25638
                     /locus_tag="ACGI6B_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751065.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            25751..27319
                     /locus_tag="ACGI6B_001619"
     CDS             25751..27319
                     /locus_tag="ACGI6B_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9751066.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            27394..28419
                     /locus_tag="ACGI6B_001620"
     CDS             27394..28419
                     /locus_tag="ACGI6B_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751067.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            28412..29356
                     /locus_tag="ACGI6B_001621"
     CDS             28412..29356
                     /locus_tag="ACGI6B_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751068.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            29715..32372
                     /locus_tag="ACGI6B_001622"
     CDS             29715..32372
                     /locus_tag="ACGI6B_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751069.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            32587..33216
                     /locus_tag="ACGI6B_001623"
     CDS             32587..33216
                     /locus_tag="ACGI6B_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751070.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            33351..33677
                     /locus_tag="ACGI6B_001624"
     CDS             33351..33677
                     /locus_tag="ACGI6B_001624"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9751071.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            33693..34982
                     /locus_tag="ACGI6B_001625"
     CDS             33693..34982
                     /locus_tag="ACGI6B_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9751072.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            35006..35335
                     /locus_tag="ACGI6B_001626"
     CDS             35006..35335
                     /locus_tag="ACGI6B_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9751073.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            35483..36811
                     /locus_tag="ACGI6B_001627"
     CDS             35483..36811
                     /locus_tag="ACGI6B_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9751074.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            37134..37988
                     /locus_tag="ACGI6B_001628"
     CDS             37134..37988
                     /locus_tag="ACGI6B_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFG9751075.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     IEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            38301..39173
                     /locus_tag="ACGI6B_001629"
     CDS             38301..39173
                     /locus_tag="ACGI6B_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9751076.1"
                     /translation="MYNSSVGLEQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQS
                     SNNYKLESLFITSNIKTRDTMILVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGN
                     SGGDNYTFGLYERYDLDSLVKFVKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVD
                     FYILDSPYSEMKDAIRMGVLEKRIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVE
                     KSNVPILFIHGTKDTVCNYQNSKKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNK
                     IFKFIDSNVLSDKS"
     gene            39376..>39800
                     /locus_tag="ACGI6B_001630"
     CDS             39376..>39800
                     /locus_tag="ACGI6B_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751077.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKE"
BASE COUNT        15754 a         4100 c         7100 g        12846 t
ORIGIN      
        1 tatataataa taaagaaaaa atgtataata tttaacatta aaaaagatat ttcataaaat
       61 taaaaaacaa agtgaatttg aatactattt aaaaaataaa ataaatatat aaaaaatatt
      121 taatataaag gtggaaataa gttgaaaatg taaagtcatt ggaaaaatat agttgaattt
      181 aatgagcaaa aatgttattg ttttatcaat aaagtgttaa aataaaaact ttcaatatca
      241 ttttagggag gatgatttca tgcaaaaaag tgtaaagaaa gcaaaagtaa cgggctctat
      301 gttagctatt tttggtgttg caagcgttct ttttagttca catgcaggag gaggatttgc
      361 tacagggaat caagaaactc agtactatgt gcagtatgga tggacagcac cacttatggc
      421 aattttagca atgattatac ttactgctac catgagagaa gttatcataa tgtataataa
      481 taacaattgt aggaattata aggatttatt ttgtgagctt tggagaccat atccaaaact
      541 tgaaataata tgggagatat attactatct aatggtttta atagctgtaa gtgctgttat
      601 agcaggtgca gcagcagtat ttcaaagtat aggagtaaat tattttgtag ctgtatttat
      661 tattggagtt gtacttcttg tctttactat atttggtgct atgcttgtat ctaaagcagc
      721 tacagcaatg acaatagcta ttctagtatg tactcttact atttttattg ttggtataaa
      781 agctaaagta ccagaaataa ctgaaatatt atcaaataga acgagtttca cacctggcta
      841 tatcaagcca atacttaata cttttattta tgcaggtttt caatcggttg ttatacctac
      901 acttgcagga tgttcaagac cacttcttaa gaactcaaaa gaagctacta aggcaatgat
      961 aatttcattt gttatgaatg caattgcatt aggacttgct gtaactatgc taatgggatg
     1021 gtatagagaa attattgctg ctgggcaaac aacactccct actctttatg ttgcaggtca
     1081 atctggaaat catactatat acataattta taatgttgca ttattccttt gtttaatgtc
     1141 tacaggagta actactatat ttggtcttgt aaatagattt gaagaccata aagcactttc
     1201 tttcctttct tcaagaatga aaagaagggt atttacagct tgtgcaataa tggtagtgtc
     1261 aatgcttata tcacttacag gattaagtaa tattgttaaa tatggatatg gatattgtgg
     1321 ttatcttggt ctattcacta tagtaattcc atttcttaca ctaggacatt ataagaataa
     1381 gaagtttgca aaggaaaatc cagaagctaa atggccagcg gaattaaatg aaaatgtaaa
     1441 ttagatgtag tagcaaaaaa tattgctaaa gagaatacta taaataagaa tactatagat
     1501 aatatatctg catgtttatt aagattattt gcaaaatcta atattaaaca actataaaca
     1561 agcaatataa aactaagaat aaaagataaa aagagggtat ctcaaaatgg acttttagtt
     1621 tataaggcac tcttttttat atgaagtcaa atatattttc attattttga caataaaaga
     1681 gactaatcct actttggata gcctcttttt tctttaatat aaaatagtat aacagcaaag
     1741 tatagattga tacatgtttt ctgtagcttt ctatatatta cagtgtttgt gtggcattgg
     1801 tattttaagc aatctataaa ttataattct ataattcttc tactaatttt agattctata
     1861 gccgtctcta tttcacttaa cattcgttca tttttagggt caataaaagc acatgtatag
     1921 ccttcttcac cagctctacc agttctacca atacggtgga tgtagtcttc tggtgtttct
     1981 ggtaaatcgt aattatatat atgacttact ccagatatat caagaccacg agaagctaca
     2041 tctgtagcta ttaagtattg tatatctaaa tttctaaatg atttcattat tctttctcgt
     2101 tttgattgag caatatcact atgaagtttt tgacaattat atcctcttat agcaagagct
     2161 tcttcaagat tgtcaaccct tctcttagtt ctacaaaata ttattgccat aaaaggatta
     2221 tcttcatcta gtaccttaca taaggcatct aatttttttc tatctgtagt ttctactact
     2281 tcttgtttta tattattaag agtgatttct tctttttgaa tagaaactac aacaggattt
     2341 tttgtgtatc tataagctaa tttttttact tgagaattta tagttgcaga aaagcatagt
     2401 gtttgctttt tgtttgaagt ctcttttaaa atagcttcca cttcattttt aaatcccata
     2461 agcaacattt ggtctgcttc atctaaaaca aaagtcttta acttactaag gtctattgtt
     2521 tttctattta gatggtctaa aagcctacct ggtgtagcta taatcatatg aatatttcct
     2581 tttagtttct ttatttgaga accaatatct tttcctccat atgctgcaag aatacttaca
     2641 tctttagatt ctttaagttt catagcttct tctgttattt gaatagctag ttctcttgta
     2701 ggagaaagta taagtacttg tatatcattt atatctaatg atatattttc aaatatcgga
     2761 agtaaaaaag ctagtgtttt tcctgttcca gtttgagctt ctgctattac atcttttcct
     2821 tcttttatta gtttaatact ttctttttgt ataggtgtag gtgttgttat tcctgatttt
     2881 tttagaatat ttattgtatt ttcactaatc cctaagcttt tgaaatccat tgttatctta
     2941 cctttcatga aaaattttct tttttattaa ccaattaata atatcatatt tgtaagtatt
     3001 tttagtaaat tttttaatta ttatttatat agcttaaaaa tatattaaga tactttaaaa
     3061 tggaatgtaa cttaaattaa attttagtag gaattttata ggtttatttt taaaggtttt
     3121 ttgaaaataa atctatttaa aaattaaatt aaaatgcaag aaaaacataa taactttcat
     3181 ttttaagaag ttttcacaaa aaagaaaaaa tatagtgcaa taaatgatac aaagtaatat
     3241 aatagattac aagatatgaa aagctaaaaa aataaattgc aaatgaaatt aaaatgaata
     3301 aaaaataaaa aaataatcat aagttagaag tatcaatgaa aaataagact taatgttttt
     3361 aaaattaaaa gtatactaag gggggcatta atatgtgcgt tatatcaaaa gatagagcaa
     3421 aggaaataaa aatagtagat actactttaa gagatggaga gcaaacagct ggggttgtat
     3481 ttgcaaatag agaaaaaata atgattgctg aaatgttaag tgacctaggt gttgaccaaa
     3541 ttgaagttgg tatacctact atgggtggag atgaaaaaaa tgtaataaag cacatatgta
     3601 gtagaaattt aaaatcagat ataatggctt ggaatagagc agtaataaaa gatgttgaag
     3661 aatctatttc ttgtggtgtt gatgcagtag ctatatcaat atcagtatct gacattcata
     3721 tagaaaataa attgagaact tctagaggct gggttttaga aaatatggct aaaacagttg
     3781 aatttgcaaa gaaaaatgga ttatatgttt cagtaaatgg agaagatgct tcaagagcag
     3841 atatagactt cttaactgaa tttataaatg ttggaaaaca agcaggggca gatagattta
     3901 gatactgtga tacagtagga gttatgaatc ctttttctat aaagaatgca atagaaactc
     3961 tttatgaaag aactaatttt gatatagaaa tgcatacaca taatgacttt gggatggcaa
     4021 ctgcaaatgc acttgcagga atagcagcag gagcaaatta tgttggtgtt acagtaaatg
     4081 gattaggtga aagagcagga aatgcagcat tagaagaagt tttaatggca cttaagtgtg
     4141 tttataaatg tgatttaaat aatatagata ctagaaaatt tagaggaata tgtgagtatg
     4201 tagcacaggc atcaggaaga atacttccta catggaaacc agtagttggg gataatatgt
     4261 ttatacatga atctggtata catgcagatg gagctttaaa agacccacat aactatgaac
     4321 catttgaccc aagtgaagta aatcttgaaa gaaaaatagt aataggtaaa cattcaggaa
     4381 gagctgcagt tgtaaataag ttgagtgaat atgaaatgta tatttctcca gaaaacgcta
     4441 ccaagctatt aaatgctata agagctacat ctataagatt aaaaagaagt ttaatggata
     4501 aagaaattct tcaattatat tgtgatatac tagcacatga gaaaggcaca acagaagaag
     4561 aagcagttag aggttcatat atttaagata taattgttta ggaggaaaat atgggagata
     4621 atatagttta taaaataatt aaaaaacata tagtagatgg agaagctgtt gctggaagtt
     4681 caattggtat aaaaatagac caaactttaa cacaagattc tactggaaca atgacatatc
     4741 ttcaactaga agctatggga atagataaag taaagactaa aagaagtgtg gcatttgtag
     4801 accataatat gttacaacaa ggatttgaaa atgcagacga ccataagtat atacaaacag
     4861 tggcagataa atatggagtt tatttctcta agccaggaaa tggtatttgt catcaagtat
     4921 ttttagaaag attttcaaca ccaggagata cacttttagg ttctgatagc cacacaccta
     4981 ctgctggagg tgtgggcatg atggctattg gagctggtgg attagatgtt gctttagcca
     5041 tggcaggagg agcatactat ataaaagcac caaaagtctg taaggtgaat ctagttggaa
     5101 aattaaataa tatggtatca tcaaaagata taatattaga agtgttaaga aaacaaactg
     5161 taaaaggtgg agtaggtaaa gtttatgaat atggtggaga aggtgtcaaa agcctatctg
     5221 ttccacaaag agctacaata acaaatatgg gggctgaact tggagcaact acttcaatct
     5281 tcccaagtga tgaaaaaaca ctagaatttt ttaagagtca aggaagagaa gacgcatggg
     5341 tagagctaaa accagatgca gatgcagttt atgatgaaga gattactata aacttggatg
     5401 aattaaaacc attagctgca aaacctcata gcccagacaa tgtagatgaa gttgaaaata
     5461 taggcaagat taaaatagac caagtggcaa taggaagttg tacaaattct tcttatgaag
     5521 acttaatgaa agtagcacaa atcctgaaag gaaataaagt acataaggat gtgagtttgg
     5581 taatagcacc aggttcaaga caagttatgg agatgattgc tagaaatggg gctttggcag
     5641 atataataag tgctggagct agaatactag aaaattcttg tggtccatgt attggtatgg
     5701 gtcaatctcc aggtactgac tctgtgtctt taagaacttt caatagaaat ttctatggta
     5761 gaagtgggac tttgtcagca caagtatatt tagtaagtcc agaagtggca gcagtaagtg
     5821 caatcaaggg agtgcttaca gacccaagag aatttgatat taaatttaca aatttagatg
     5881 taaatgagtt tttgatagat gatagcatga taattaaacc tgctgatgta ggaagtgatg
     5941 tagaagtagt tagaggacct aatataaaac ctttccctct taatacagag ctaagtcagt
     6001 ctataggtgg aaaagttata ttaaagacag aagataatat aactactgac catataatgc
     6061 catcaaatgc taagttactt ccttttagaa gtaatatacc gtatcttgca aactattgtt
     6121 tcaatactgt agatacagaa tttcctcaga gagcaaaaga caataatggt ggatttatag
     6181 ttggtggaga taattatggt caaggttcaa gtagagaaca tgcagcttta gcaccattat
     6241 atctaggtgt taaaggggta atagttaaga gctttgcaag aatccacaag gctaacttga
     6301 taaatagtgg tataattcca atggagtttt gtgatgagaa agattatgaa aatatatctc
     6361 ttttagataa tctggagata ccaaatattt tagacaatct aggtagtgga attttagaag
     6421 ttaagaatac aactaaagga acttctttta aggtcaaagt agaattgtca gcaaaagaag
     6481 ttgatgtatt aaaagctggt ggaaaattaa actacactaa aaatcaagct aattaatatg
     6541 aaaagaggga atattgaatg tataaagtga cacttatacc aggagatgga ataggaccag
     6601 aagttgcgaa agctatgaaa aaggtagtag aagctactgg agtagaaata gaatgggaag
     6661 aagttaatgc aggagaggca gttattgaag agtatggtac tccacttcct gaatatatta
     6721 tagatagtat aaaaaagaac aaaatagcta taaaaggccc aattactact cctgtaggta
     6781 aaggatttag gagtgtaaat gtaactttaa gacaggcatt agatttgtat gtcaacttaa
     6841 gacctattaa aagttttaag gggataaaat caagatatga agatgttgat ttagtggttg
     6901 taagagaaaa tacagaagat ttatatgctg gaatagaaca taaaataggt gattatgcag
     6961 ctgaaagtat aaagataata acaagaagtg caagtgaaag aatcgttgat tttgcatgta
     7021 actatgttaa agataataag agaaaaaaag ttacagctat acataaggca aatataatga
     7081 aaatgtctga tggattgttt ttagatgtgt ttagagaagt tgcatcaaaa catggtgtag
     7141 aatatgatga tttgattgta gatgcagcag ctatgaactt agttttaaat ccagaaaatt
     7201 atgatgtaat ggtaatgcca aatctttatg gagatatact atcagattta ggtgctgggc
     7261 ttgttggagg tttaggtata attccaagtg caaatattgg taaagattgt gcgatatttg
     7321 aagctgttca tggaagtgct ccacaaatag caggtcaaaa taaggctaat ccaacagcac
     7381 ttattcaatc atcagtaatg atgcttagat acttaggtga atatgaaaat gcacaaaaaa
     7441 tagaaacagc tttagaaaaa gtatttcttg aaggaagtaa attgactgta gatttaggtg
     7501 gctcagcttc tactacagag tttgcggatg aggtgtgtaa gtatattgta tagattttat
     7561 ttagggaaaa agtaaaatat agattgaaga aaagaggctt tctcaaaata gagataagcc
     7621 ttttttattg gaatgataaa atatatttga ttttatacaa aagagagttc ctcataaacg
     7681 aaaaagttaa ttttgggata tccattttaa ttttaaaatt aattgattaa tacgcaaaga
     7741 aagattaaat aaatttatta tttttaattg aactttcaac caaaatatgg taaaatttaa
     7801 aaagttgaac tttcaactaa aaatgaaaat attgattatg tgaaattcta taaaacagaa
     7861 actatttgac tttagccatg gtagtttcag tggaggtgtg tttgattaag actttagata
     7921 gtaatatatt aagagaagta ggtactttat ctagagcagt aaattcaata aatgatatta
     7981 agtacaagga attaaagtta caaaaaggac aatttacatt tttgacaaga atctgtgaaa
     8041 atcctggtat aaatcttgtg gaattatcaa atatgttaaa ggtagacaag gctacaacaa
     8101 ccaaggctat acaaaaatta ataaaggcag gatatgtaga taaaaagcag gataagtttg
     8161 ataaaagagg atataattta actccaactg ataaatcttt ggaagtttat gagttgataa
     8221 ttgaagaaga gaatagaagt atagagatat gttttgataa ttttacagat gaagaaaaac
     8281 aagtggtaac taagttatta gaaaaaatga gcaaaaacgt agaaaatgaa tggtttaaag
     8341 taaagagata gtaaataaat acattaactt aaggagatat ttttatgatt agaaaagcga
     8401 atatgaatga tttagaaagc attatgaaaa ttataaaatc tacagtggaa gaaatgaaga
     8461 cttataataa tacacaatgg gatgaaaact atccattaga aaaagacttt gtttcagata
     8521 ttaagaaaca agatttatat atttatgaag tggatggaga agtagcaggg tttatatgtc
     8581 ttaattatga agagccagaa gaatatttag gcttaaattg gtcatctaat aaaaaagcaa
     8641 tggtaataca tagaatggct gtaaatccaa actttagaaa gacaggaata gcatctaagt
     8701 tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt atcatactta aagagcgata
     8761 catattcaat aaactcaaaa atgaatttat tacttacaaa gtgtggtttt ataaaaatcg
     8821 gagaaatgag ttttttagga aaggagaaat ctttctactg ttatgataaa atattataaa
     8881 gagcatttat aatacaaggg gtaaataata gattttcagc attagtaagg gcataatata
     8941 acctagtata tgctattatg aaagggatga tttatatgat tggatttcta aagaaaagaa
     9001 gaagtattag aaaatacaag gatgttgaag tagaaaaaga aaaattagat aaaatattaa
     9061 aagctgctct tttagctcca tcatcaaagg ggcttagaac atgggaattt atagtggtag
     9121 atgataaaga aaagctaatt aatttatctc aatgtagaac taaaggtggt ggatttttct
     9181 taaaaaatgc acctttagct attgtaatta ttgcagataa agaaaaaaat gatgtttgga
     9241 tagaggatgc aagtatagct gcttcataca tacaacttca agctcatgaa ctaggtttag
     9301 gttcttgttg gatacaagta agaaatagaa tgcatgatga taatatagaa gctgataagt
     9361 acattagaga agaattaaaa gtacctagca agtacagtgt agaatgtata atttctatag
     9421 gatatcctga tgaagaaaag aaagcatacg gtgactctga tttagactat aaaaaggttc
     9481 attttaacaa cttttagtat ataatatgtt gtattaagac attatatttt tttctattgt
     9541 taatatatac ttaaaattgt aaaaatatat ctgaatttta ctaacttatc taggggttat
     9601 gtacaaggat tttaaagata aatatagaaa ttaatgttta taatcgatac ataacttgag
     9661 taacaaaatt taaggaggtt aaagtatggg tatatttaaa acaaaaatgg atgaggactg
     9721 gaaagtaaac tacattaaag agtttaatga aatgagagat tcttatgaga gtaaacttca
     9781 aaaaaaacag tttgaggtag atagtttgaa atcagaattg gatagactaa gaagctataa
     9841 aaatagcctt aagccaaaag aaaaacaaat tactgatgat gatataaata atataaagag
     9901 tttaagaaga gatggcttaa gctataaaga aatttcaaat caaacaagtt ggagcaaagc
     9961 aactgtaagt agggtattaa atggacttta cgattagcat atacatgggg tgatttaaat
    10021 tgggatgaag tatagttaca aaaaatgcat aatagattct attttattga tgtttatagt
    10081 tcaaatatta aggatgattt tgaattatgt acttctaagt caatttgaat ttactcttga
    10141 aaattttaat attataaact taatttcttt tacattagtt ggtttatcgt taattttatt
    10201 tttaaaagat aattctttgt ataacaaggt tagaaataga aaaatcacag aagcttttga
    10261 agaaaataaa aacaatatac ttattgaaaa gtgtaagctg atactttttg ttgtggtttt
    10321 atctttagca ataattgtaa cttattgtac taagggatat gtacttttta atgtaactat
    10381 gatgactttg tctgtattga tagtcccaat atttgaagag ttatttttta gagaatatat
    10441 atggaactat ctaagtaatt ttataaagtc aaagagtaaa attatctgta taacatctat
    10501 actatctgga atttataata taggatacat agatgttata agaaattatg taatattgta
    10561 taacaattca agctacacct ttgaagttat tatttcaaaa attatgatag gtactgtttt
    10621 tggtattgta cttggattag ttaagtatag gtttagagat gtagggtttt gtatattatt
    10681 gaggagctta tttgctatat ttattaggta gatagtataa tcggtttata aggttgtttt
    10741 aaatttaatt ttaaatcaac cttattttat ttgtgtttta taaagcactt atatattaat
    10801 aactaataaa tttaataaaa attatttgaa tatttttaaa atatataaat atgtattgac
    10861 atataaatat tatgaattac aatttatata aactaataaa aattaaataa tattaatcaa
    10921 cttattgaat ggaggaataa aaatgaaatt tgtgactttt tgtagtagca atgaagaaaa
    10981 aataggtgta tttaattcag aaacaaatag tatatatgaa attaatagtt taggattaag
    11041 taaattatat actgatatga atgattttat tgaaaacgta tctacaggag atttagaaaa
    11101 aataaaaaac aattcatttg aaaacgcaaa gtgttataag ttagaagaag ttaaattatg
    11161 ttccccaatt gtaagaccta aaaaagatat tatatgttta ggtctaaact ataaagacca
    11221 tgtcaatgaa atcccagatg gagttataaa aaacgtagtt atgccagatt atccaattta
    11281 tttttctaag agagcagaca aaataattgg agtggatgat aagataagct tacatggaga
    11341 tttggtagag aagttggact atgaatcaga acttgcagta atcataggta aagaaggtat
    11401 aaatatatca aaagaagatg cttatgaata tatatttgga tatacaatag taaatgatat
    11461 aagtgaaaga gcacttcaag ataaacatgt tcaatggttt agaggaaaaa gcttagacac
    11521 acatacatct atgggacctt gtatagtaca taaagaagaa tttgaacatc ctttaaaatt
    11581 agatataagt agtgtagtaa atggagaagt aagacaagat tctaatacag agtactttat
    11641 atttgatata cctactataa taaatgattt atcaagagga atgactctaa agccaggaga
    11701 tataatttca actggaactc cagcaggggt tgctatgggg atgaatcctc aagtatattt
    11761 aaagcatggc gatatagttg aatgtaaagt cgaaggaata ggagttttaa aaaatatagt
    11821 tgattaatgg ataaatatgc catataattt tattagtacg aataatttaa ttaatatagg
    11881 attaatcaaa cttatgatta atttattata gagagggatg agtatatggc aagagaacta
    11941 gaattaaagt atggctgtaa tccaaatcaa aaaccatcaa agatttatat gaagaatgga
    12001 gaacttccta tagaggtatt aaatggaaaa cctggataca taaatttttt agatgcattt
    12061 aatagttggc aacttgtgaa ggaattaaaa gaagctacag gtcttccagc agcaacttca
    12121 tttaaacatg taagtccagc aggagcagca gtaggggtac ctttatcaga tactttaaag
    12181 caaatatatt ttgttgatga tttagagctt tctccattag cttgtgctta tgcaatggca
    12241 agaggagcag atagaatgtc ttcttatggc gattttatag cactttcaga tgtatgtgat
    12301 aaggagactg caactattat agcaagagag gtttctgatg gtataatagc tccaggatat
    12361 acagatgaag cccttgaaat attaaaagga aagcgtaaag gtaactataa tattgtaaaa
    12421 atagatgaaa attatactcc tgaacctatt gaaacaaagg atgtttatgg aattactttt
    12481 gaacaaggta gaaatgaaat attaataaat gaagacctat taaaagatat tccaactgat
    12541 aataaaattt ttacagatag tgcaaaacgt gatttaatta tagctcttat tacattaaaa
    12601 tatacacaat caaattctgt ttgttatgct aaagatggac aagtaatagg ggtaggtgca
    12661 ggtcaacaat cacgtattca ttgtactaga cttgcaggta acaaagctga tacttggtat
    12721 cttcgtcaac atcctaaagt attaaactta aaatttaaaa aggatatagg tcgtccagat
    12781 agagataaca ctattgatgt atatctttct gatgattata tggatgtgct tgctgatggc
    12841 atatggcaaa acttttttga agaaaaacca gagcctctaa ctggagaagg aaaaagagca
    12901 tggcttaaaa ctttaacagg agtagcactt ggttcagatg catttttccc atttggagat
    12961 aatatagaac gtgcaaaaag aagtggggta agttttatag cacaaccagg tggttctatt
    13021 cgtgatgata atgttatatt aacttgtaat aagtataaca tagtcatggc atttactaaa
    13081 aatagattat tccatcatta aaatactgaa agtataaaga taatatagat taagtaaaaa
    13141 tctacaacaa gttttgttgt agattttttt atttgctgta taattttaaa tagaacttta
    13201 tttttagata ttcaatttgg aggttgatat ggctttaaat ataagaaact atagcctatt
    13261 atgtaatcaa ggaacagcta aatttaatga agatgtagtc ggtataaatc catttggggc
    13321 atgggtttta gatggtgcta caggacttaa tggaaaaaat ttagtgtcaa ctgaaagtga
    13381 cgcaaggtgg tatgttcagt ggtggaatag gtatttatat aaaaatataa ataaagaaga
    13441 atcattaaag agtattatta atactggaat aatagaagta aaaaaagagt atgaactaag
    13501 attaaatggg tttaaggttg aaaaattaga tttaccatct agttctatag ttgttgtaaa
    13561 atttcatgaa aataaagtag agtatttttt attaggagat tgtgctttgt ttattaaaaa
    13621 tggagattcc aaagttataa aagataggag tatttgtaaa tttgataata ctgtatttga
    13681 tgaaatgagt aaactttcta acttggaaga gatggctttt gatgagatta gaactagtgt
    13741 tatggataca atagtaagca atagattgaa gaaaaataca aaagagggct attggatatt
    13801 ggattttgat gaggaagctg tggaaaattc tatatatgga tacattgaaa taaaaaaaga
    13861 ttttcaattg atgctaacga gtgatggttt tacaagtgca tgtgatagat ataatctaat
    13921 aaaagaagaa gaattgatac gtatagcaga aaaatttggt ataggatata tacataataa
    13981 agtaagggat tttgaagata atgactataa agcggttaaa gtgccaagat ttaaagtaaa
    14041 agatgattca tcttgtttat atttagatat atataatgat tagactgtat aaaactattt
    14101 aattttagaa aggaggcaag tgaaaagtga aaattcttca tataataaca caaaaaccaa
    14161 atagtacagg tagtggtata tatttgtctg gtatgataaa agggttcgaa aaaatcggtc
    14221 ataagcaagc tgtaattgca ggtatagatg taaatgatga tgtaaattgt tttccaagtg
    14281 aagtaagttt ttatccagta aagtataatt gtggggagtt aaattttcct gttgtaggta
    14341 tgagtgattc tatgccatat gaaagtacaa gatataaaga tttaaatata gacatgataa
    14401 atagattgaa atatcaattt aaagtaaata tagataaggc tatgaatgat tttaaaccag
    14461 aacttataat atgtcatcat ttgtacctct taactgcatt tgttagggaa atggtaaaag
    14521 atataaaggt tatgtctatt tgtcatggaa cttgtttaag acagttaaat accattgatt
    14581 tagagaaaga atacataata gcaaatataa ggaaattaga tttaatattt gctcttcatg
    14641 aaaatcagaa atatgatatt ataaagactt ttggagtaag tgaaagtaaa gtagttgtaa
    14701 ttggtagtgg ttataatgat gatatatttt ataataaaaa ttataaaata aaggatgata
    14761 aaataaaaat agtatttgca ggtaagatat gtaaatcaaa gggactgatt ccatttataa
    14821 aagctatatc taaattaaaa tatagtaaag atttaataga agtcaatttt gcaggtactg
    14881 gaagcgatat cgaatcatat aatgagatag ttaaactggc aagtaagtct ccatttaaaa
    14941 tgaatttcct aggaaagtta gaacagagag atttagctga gctattcaac aggtcacaaa
    15001 tatttgtgtt accatcattt tatgagggat taccagttgt agttttagaa gctttatctt
    15061 gtggcacaga tgtaatcact actgatattc ttggtgttaa agagtggatt ggtagtgaga
    15121 ttaataattc tggaaagata gaatatgttt ctctaccttt tatggagaaa gaaggtattc
    15181 caaaagatga agagttatat gattttgaaa ataatttgta taatgcaata gattctaaga
    15241 tacaatcttt attgaataac tcaaataaga aaacatctgt agatatgtca aaaaaaacgt
    15301 gggatggctt agcatataga ataaatgaag ttattttaat ggatgaattg tgtttagtct
    15361 aaaattattt aaattataaa aaagtgtaac tatttttaca ctatttttaa atgattttat
    15421 attatactaa tactaaatat atttgtttta gggaggtaat ttatgaggat tagtaaaaag
    15481 gtattggcgt taggtatttc agcattactg ttaagtgtta gttttccaac atcaataaac
    15541 gcactagata agattgaaaa tattcaagga gtagataaat atgaaacagc tgggcttatt
    15601 tcagataagc aagattttac aacagcagtt cttatcaatg cagactctac aatggtagat
    15661 gggattgctg caagtggtct tgcaggagta aataatgcag ctattttatt aactaataag
    15721 gatgatatac cagaagcaac acttcaaaga ctgaatagag ttactaagat atatgtaata
    15781 ggtggagaaa attctataag taaagatgta gaaaaaatgt tacttatgag aagaatgcaa
    15841 gttataagaa ttgatggtgt agatagagta gatacaagtt ataaaattgc aggtgaaata
    15901 gaaaaaatta aaaacagtga taagatgttt ttagtaaatg gatttaagga tgaagctgat
    15961 gcagtaagtg ttgcatctgt tgcttataga gatggagctc ctataatatt gacaaaagat
    16021 ataccttcag cagaagaaga cacagactta gacccatggt ttggagtttc tcctgttcca
    16081 gtatatgcta ttggaggaga gtctacatta agtgattaca tagtgagcag atatagagca
    16141 actagaattg gtggtgttga tagatatcaa actaataaaa atgttattga aaagttctat
    16201 aatggagcaa aagagttcta tataacaagt ggagatgatt tagtttatgc tttagttgca
    16261 tcaccacttg caaaaaatgc tcctgttgtt ttagtttcaa ataaaagtga taagagtata
    16321 ttaagtggag ctagtaaagt aacagcaata ggtataagtg ataagagtat tatagagcaa
    16381 tgtttggatg ctgtaaagaa gtaatggagt gttattagta aaatgaatat atacattgaa
    16441 aattagtaga ttgttcattt tataatggta taaaatatat gggaagaggc tatcaaatgg
    16501 agataagcct ctttttcatt gttaaaatta tgaaaagata ctttatttta tataaaaaag
    16561 tatcttatga actaaatgtc tattttgaga cactatctac ttttcttata ttatattatt
    16621 ttttatatgg aataaatttc ataagtatta tcttccttta atttttctaa ccaaaccatc
    16681 tctaaaatat ctagttcttc taaatagtct ttctctgttt tgttatcatc atgttttata
    16741 agttctaaaa cttcaaatgt aaatgctttt tcaccatatt ttttccattc attatttaat
    16801 tcttttatca tacatgagcc agcaccaagc tgaaatttga gagagttaaa tttgcttttt
    16861 atgtcattac tttttccaag gtaaatcttt ttagtaatat cattttttat tatgaataca
    16921 cctgtgtcaa atttcatttc tttgtataat tgttttaatt cacgttttct atccatgatt
    16981 ttagatacct ctctttataa ttgatatgta atatgtaagt gtaaaaatat attacatatc
    17041 aattataaag agattaattt tatatgtaaa tagtttttat aaatttaatt atgaattagt
    17101 catcaataaa caagaaattt agctgtaaaa atccattata aaggctatta taaattttaa
    17161 tatgctaaaa atataaaaag aatagttgac agtgtagaaa atatggaata aactaaaagt
    17221 gtaatagtaa tatgaagtta tatattacat tgaaaggtgt gaatgattat tatgaataaa
    17281 aaaatagaat taatagggaa ttttgcaagt gattggatta ggtatagtga ttatgaatat
    17341 aaaattactg aagaagatga attatatata gttccaacag aagatgctgt gtttaccatg
    17401 tataacccat ttgatgtagc agatgatata atagtagata taattcgcat aggacaagag
    17461 gcattacaag atatatcaaa agagacaaaa gaaatgaaac taaagaaaga agtacttgaa
    17521 tttgtaaaaa agtatggatt attgggttta atatattcaa gtgtatataa tagagatata
    17581 gttggagaag aaagagtact gatgattgaa aagaattata ttacaaaaga gaatatattg
    17641 agtacagatg aatatgtaaa catgtttata ccatttgcaa gtgatgaaga tgtgtatttt
    17701 aaaaagtata gaaggggtat agatgtatca aagagagaag aatctccaaa gttttttgga
    17761 aagagaccat tgattttaga tttagtattt tcaaagttct acactgaaca actagattgg
    17821 attgtaagtt ttgctaaaga tatgtgtaag cattttaatc aaatttgtgt atataaaaat
    17881 acatctaatc tattaactga aagtgttact attatgtctg gagggtttaa tcctcaaaag
    17941 ataggatttt ctataagcca attagataaa ccaattattg catgggagtt tgattctttg
    18001 aaaacagcta ttgagactat ctatgccttt gcaattacaa gtgaaccttc ttctttaaat
    18061 aaatgcaagt actgtggtaa aatttatatt cctacaaatt ctaaatctca atactgttca
    18121 ccttcttgta gaaattgctc aaatgtaaag aaaagtagaa gtagagccaa aaaagataaa
    18181 taaaattaat aaatatatca ttgtttagat tttagtaaat aactactgaa ttaaaagttg
    18241 ttatagaaaa ttatatatct aaatgatggt atttttttgt tgagaaaatt gaaaagttta
    18301 attgagatag taattttgag ttgacaaaaa ccacttttaa cactactatt ataagtggtt
    18361 ggtaaaataa tatacattaa tttaagtata gcataaaaaa caaaaaaagt caatggaaaa
    18421 aataaaaaag gagaatttaa aaatgatatc acctattatt ttgagttcta taaatcagaa
    18481 tttaaaggaa atagaaagaa atgaactgct tgaaactaat atagaatctg gagattatgg
    18541 tctagctctt tctgaatctg atgtaaaaga tataataaat tcgagagata atactttaaa
    18601 ggggtatgga agaatagaat tagatataaa agttacaaaa cagttaatag aaaatatata
    18661 tacatcacaa tatacaaatg tggacaatta tttagaggct attaatgata tgcaagaaat
    18721 tttttattat ttaaaaaatg aaactgatga caagatatgt gatgatgaga ttatagaaat
    18781 actgggtgaa ttttatgaaa aattttcagg caatatggac aatgtaagag gagaagcaga
    18841 cgaatttgca aagaaattta aatttggaga agtttaatat atgataggag agattttgta
    18901 tgttaaataa tttaagtaat attaatataa acgaaaataa tttaataaag aaccaatatt
    18961 ccatatctct acttaaggaa tgtctaaatt gtaaagtgat agatgaaaga gaagtatata
    19021 atatacaaca agaagtatct ctaatactta tggacttgat taaaaaatat acaaatggtc
    19081 aaagcacatc agtaaaaaca gaagttgccg aaaaactgtt gatttcaata tggtatgcta
    19141 ttgatgcata tataaataag cttggagata tagaaaaaag agttgtggag ttgactgcta
    19201 agaatgttaa aaaagtgtac cttggaggca ttgaaatatt agaggaagag ttagttaggc
    19261 taaaagattt ttataaacta atgatgagta gaaaattgga tactaattca atagcatata
    19321 atgatacctt gatggagtta tcaagtttct ttaaaagtta tattgtaaaa tttgaagctc
    19381 aggacgttcc agtaagtata gattatccat tggcactaga tgatatggat gttaagggta
    19441 tttattatgt gaagaattat attgagagta tagatataga aaatagattt tgtaacctat
    19501 ttaagaaaaa agatagagaa aaattatttt atgactatgg gatgacttat aaaatagatt
    19561 gtgatgttat acttgtaaat ttatttgaac ttattatgaa taattgtatt ttttctacta
    19621 ttttaggaaa tgaagtgact aatttagaaa tttcaaaata tgagtatgaa tttttagaaa
    19681 aacaatttaa aaatatgtac aataatatta ctgaagataa agttatagac tctgaagaaa
    19741 gtaatataat tgaattgatt gaagtagatg atagttttaa cactgaagta gatagtaatt
    19801 tggattataa tgattttgaa gaaattaaca gagaaattag aataaataaa aaaataactt
    19861 tactacttat ggaagcaatt gaagtattaa taaaaagatt aagcatagaa gatgaagatg
    19921 taataagata tattaaaaaa tatgaaaaga tatttattga atctgtgcta agctcaataa
    19981 aaagtaacac ttttaaaagt atggtaactg tgagtaaaaa taaaaaggaa cacttagaag
    20041 attatattgt tgatgatgaa agtaaattgg atgatgaatc gtttagagag atattcaatg
    20101 agatattaga tagtacatct ataattgaaa aaataagaat aataaaggaa aatatcaatg
    20161 ctaaaaaaga ctttatcgat attcttgaat ctgaatgttt atttggagaa gagtatttaa
    20221 tgttgtttgc atcattaagt gaattagaat tggcaatatt gggtagtgta gtattttatg
    20281 aagatattag aatgagagaa attaatatat tagaatttat gttaaatgag aaaactgaaa
    20341 ctactttatg gaagatagag tacattaaat ttatgaagag gcaaagtgaa gataagatat
    20401 actccataga agagcatatg aataaaatta attaacaaaa aattgtgaat aaatagaatg
    20461 ttagtatata atataatctg taaagcatat caaaaataca tatatttgga ggaaaattat
    20521 gaaagagtat atcataaata atgttgaaga aataagagaa gaattaattg atttatctaa
    20581 gaaaatatgg gaaaatccag aattagcctt tgaagaaaag tatgcaagta gcattcaaaa
    20641 agaataccta aagtcaaaag gatttaaaat tgaagaagta gaaaatttac ctactggatt
    20701 tatagcaagt tttggagagg gtaaacctgt gattggcata cttggagaat atgatgcttt
    20761 accagagcta tcacaatgtg taagtgcaga aagaaagcca ttggtagaag gaaaagctgg
    20821 acatggatgt gggcacaacc tgttgggtgt agcaggtgta ggagcagttg tttctataaa
    20881 aaaattaata gaagaaaata aatttaatgg aacaataaaa tattttggtt gtcctgctga
    20941 agaagaaggt ggaggaaaga cagttatgtg tataaatggc tgttttgatg atgtagattg
    21001 tgcatttaca tggcatcctt ttgatataaa tgcaccttgg agaggtggaa gtctggcaaa
    21061 cttatctgta aaatttaaat ttaaagggat aacagcccat gcagctcaag ctcctcataa
    21121 tggtagaagc gcacttgatg ctgttgaaat tatgaatgtt ggagctaatt atttgagaga
    21181 acatgttatt gactctataa gaatgcacta tgttataact aatggtggtg gaagacctaa
    21241 tgtagttcct ggatttgctg aaagttggta ctttattaga ggtaaaaaag caaaggatgc
    21301 agaacacgtt cttgatagat taataaaagt agctcaagga gcagcaatga tgacagaaac
    21361 tgaaatggag tataaggtta cagatggaat atatgactat ataccaaacc aatgtttgac
    21421 tgatttagtt tatgacaata tggtttttgt tggttgccct aaaaatactc ctgaagaaga
    21481 agaatttgca aaaaagttgt gtgatactct tactagagaa gaaagattag gtgtagcaac
    21541 atcattacaa aatgataaat ctattgtaga aagctatata cacaaggaaa tagttgacgg
    21601 tgacaaagat aaaggactag ctggttctac tgatgttgga gatgtaagtt atgtaatacc
    21661 agtggctcaa tttgcaatgg cagcttggcc tgttggaatt gcaagtcata catggcagtc
    21721 atgttcatca gcaggttcaa atataggatt ttctgctatg ataaattcag ctaaagtact
    21781 ggcttgtagt gcttatgatg tatttatgga tactaaaatt attgatgaag caaaaattga
    21841 atttgataaa tcattagatg gacaaaaatt taaaccattg gtataatttt gctaataaaa
    21901 tatttctggg gggaatagct atgagagaaa aagttgaaaa agtgcttgag gaaaagataa
    21961 aaccagtatt acaaagagat ggtggagatg tagagcttat agatgtaaat gaaaatggtg
    22021 tggttttagt tagactacaa ggggcatgta gtgggtgtcc aggagtgact atgacaatca
    22081 aagctattat tgaaaatgtt ttggtgagtg aagttccagg agtaactcaa gtattaggtg
    22141 tttaatagtt agcgtttatt aaatgtatgt tatacataag tatttaataa agaagtaaaa
    22201 aaaatagtga agcaatatat atgaaaaagt atgagaaaga taataaaaca ttatagaaat
    22261 aatgaaatat ttcatatgta tatgaagaaa taagaaaaaa tagtgaagca atatatatat
    22321 gaaaaagtat gagaaagata ataaaacatt tatagaaata atggaatatt tcatatatac
    22381 atatgaagaa atagtaaaac aatatatata tgaaaaagtg tgagaaaata ataaaatact
    22441 atagaaacaa ttaaatattc tatataggta ttaagaaaca tgaaagaaat aatggagtat
    22501 tataaataaa tatgtaaaag tgtgaaagaa ggatgtagat aaatttgtct tatacaaatt
    22561 tacctacagt atttcttaca cactttttat tttactatcc agccatacca ataaacatac
    22621 ctataaaata tggaactaac catatagtcc atacaactat actaaactta tgaaagaatt
    22681 ttttcttttc ctcatcatct ttgtatagga caaaagtagc ccaacttgca tgaaaaagca
    22741 taagcacaat tgcaagaaga cctgtaatac tatgaaggtt ttgtgataaa gcagatttta
    22801 cttcaaaagt atggctgttg gctatgttac tcattgtaaa tgttcctaaa gtatcaaata
    22861 taaaaccaag ccaaaatata attacatgtt tcttttttaa aatttttgcc tttctttctc
    22921 caaatacacc tatagtgtag aaaattaaag ctgatgttat aaatactatt gcaagtatta
    22981 atttataatt cataattatc tctcctttac ttttgccaat atatttctaa gattttttac
    23041 taagtcactc aattcgtttt catcacaatt tttaaaaata tcatcaaaac tttcttgcat
    23101 tttaatttta aaatttgtgg caaattcaaa tcctgttgtt gtaaacttaa cgtatgtatt
    23161 tcttttatca ttagatttct tgaatttttc tatatatcca atctgttcta atctgctgat
    23221 tatgcctgaa accgtacctt tagctaaaga catttcatca catagttgtg atattgtaag
    23281 ttcttgatta tgagctatca gttttataac tataatctgt tggtgtgtta atccattttc
    23341 tttaaaattt tcttctactg tgtacatagt tttagaataa agttctttta agagcatggc
    23401 tattctaaaa cagtcataat tacttttcaa taaaatcacc tcttaaatgg tttgcataca
    23461 aactaatata atatactttt cttatatagt caacctaaaa ttttataatt ttatagaaaa
    23521 taatgaagaa tagaatataa aaattttatt ttaggtataa ataaaataat ttgatgaaat
    23581 tttaacaatt tttaaaaagt ttgtttacac agttaataaa tgatgctaaa attaactcat
    23641 agataaatat agaaaattta attttgttta atgataatgg aaccacgaag gttttaacgt
    23701 tactttacgt ttgcttttat gtggtttttt ttattgcaac aaaggggggt tggggtttgt
    23761 taaaggatat actgaaaaag ttgttgcagt ttattttagt aatgttttta ttatctttta
    23821 ttgtttttta catggcaaga cttgctccag gagacccatt aatctcttac tatggggatg
    23881 gagtagaaag aatgagtaca caagaaaaag aaaatgctat gaaaaaactt ggtttggatg
    23941 aaccaatata cagtcaatat ataaaatgga tttcaaatgc atcaaaaggg gaatttggaa
    24001 tttcatttaa atataaacaa aatgttactt ctgttataaa tgatgtctat ataaacacta
    24061 taatattagg tggttcagga tatatactta cctttgtgct agccttattg ttaggaatat
    24121 tttgtacatt acatgaagat aagctcattg atagaatcat atgcaaatta ggaacaataa
    24181 ccaactgtat tccatcattt tgggttgcct tagtactcat acttatattt agtataaact
    24241 taagtatact tccaagtagt ggtgcttatt caatgggaga agagtccagt atattaagta
    24301 ggatatcaca tctaatctta cctcttacag tacttatatt aagccatcta tggtattaca
    24361 catatatgat aagaaataaa ttactagagg aaataagaga agattatgtg cttttatgta
    24421 aagcaaaagg tttaaataat agaactattg tttttaagca ttgcctgaga aatataatgc
    24481 catcatatat tagtataatg gccatttcga tacctcatat actaggagga acttatgtag
    24541 ttgaaaaagt tttttcttat cctgggttag gaacactttg ttttgaaagt gcaaagtatc
    24601 atgattataa tatgttatta gttttatgct taataacagg ggctttagta gtatttggga
    24661 atatgttagc tcaaattata aacaataaaa tagaccctag aatgaaatat gatagaggtg
    24721 atactaatga agccacaata tagtgatttt gaaattgtag gagaaaatta tattttagta
    24781 gcagaagatg aacatataga aacaaagaaa accttatcta aaaaaattaa agaattacca
    24841 tatatatcaa ttattatttt atcaatcata gtcattggaa gtgtattctc atcttttata
    24901 atgacacatg aaccaacata catggattta gcaagttcta atttagctcc aaataaaact
    24961 tttttatttg gaacagactc aatgggtaga gatatatatt ctatgatatg gtatggagga
    25021 aaaatatctt tatttatagg actattttcc actgtaatat caactgttat aggaattgtt
    25081 tatggtagta taagtggttc agcatctgaa cttgtagatg atgcaatgat gaggtttaca
    25141 gaaatcatac ttagtatacc ttcaatatta attataatat ttgtacaagc tatacttgga
    25201 aattcaaatc cagtatctat gtccatagta atagggataa caagttggat gaatatatct
    25261 aagatagtga gaacagaagt tagacaaata aaaaatagcg aatacatact agcagctaag
    25321 agcatgggag gaggattctt ctatgtatta aagcaacatt tacttccaaa ttttgtggca
    25381 tcaataatgt tcatggttgt aacaaacata ggcgcagcta ttggtactga atcgacactt
    25441 agctttttag gaattggttt accaatagaa attgtttctt ggggaagtat gttatctttg
    25501 tcagaggaag cattactttc aaatagatgg tggattatac tgattccggg tatatttttg
    25561 gttaccacat tggtgtgtat aacaaatata ggtaattata taagaaagaa taataataaa
    25621 aaatctagta atttataaat cagcatttga gtattaaata agcaaatact gaggtatttg
    25681 aaatatctat ttaaataaat ggggtcaaag ctttgaaaaa tattttataa aaagcaaaag
    25741 ggggagcatc atgaaattaa agaagttaaa agttttaagt ttagtaatga tacttagtct
    25801 aatggcaggt tgttctagtg gaggagacaa agataagaag gcagatacgc ctaaagatgg
    25861 aaaggtgctt gtttatggaa gtaatgacta tacaagtata aatcctgcac tatatgaaca
    25921 tggagaaata aattcgttaa tatttaatgg attaacagct catgatgaaa ataataaggt
    25981 ggttccttgt ctagcaaagg actggaaatt tgatgaggca acaaatacat atacttttaa
    26041 tttaagagat gatgttaagt ggcatgatgg agaaaaattt acagcaaatg atgttaagtt
    26101 tacaatagaa actataatga atccagataa tgcttctgag atagcatcaa attatgaaga
    26161 tataacaaaa attgatgtgg ttaatgataa tactataaaa attactttaa aagctccaaa
    26221 tacagcaatg cttgattatt taacagtagg agtattacca aaacatgctt tggaaggtaa
    26281 ggatatagct actgatgaat ttaatcaaaa accaataggt actggaccat ttaaacttga
    26341 aaaatgggac aaaggtcaaa gtataacact tgttaagaat agtgattatt ttgtaaaaga
    26401 accaggatta gataaagtag tctttaagat tgttccagat gataaggcta aggcaatgca
    26461 attaaaatca ggagaattag acctagctca aataactcct aaagatatgt ctaactttga
    26521 aaaagacgaa aagaatttta aagtaaatat aatgaaaaca gccgactata gaggtatatt
    26581 atacaatttt aattctaaat tctttaaaga taaaaaagct aaaggattac caaatgcttt
    26641 aagctatgca atagatagaa aagcaatagt tgacagtgta ttattaggac atggtgtacc
    26701 agcatattca cctttacaaa tgggaccata taataatcct gatatagaaa agtttgaata
    26761 taatccagaa aaagctaaac aagaaattga aaagctaggt tggaaattag gttctgatgg
    26821 aatctatgaa aaagaaggta ctaagttagc tttcgagata acagcaggtg agagtgacca
    26881 agttagagtt gatatggcta aaatatgtgc acaacagtta aaagaaattg gtgttgatgc
    26941 taaagctgtt gttgtaactg aaacagattg ggctaatcaa gatgctcatt taataggttg
    27001 gggaagtcca tttgacccag atgaccatac atataaagta tttggaacag ataaaggtgc
    27061 aaattacagt gcttactcta atcctactat agataaaata cttcaaaaag caagagaaac
    27121 agaagataaa gatgaaaaat taaagttata taaacaattc caagtagaaa tgactaagga
    27181 tatgccttat acatttattg cttatataga tgcaatatat gttggaaaac caaacataaa
    27241 aggattaaca cctgatacag ttttaggtca tcatggtgta ggtatattct ggaacatagc
    27301 tgactggaca attgaataat actgaaatta attagaaacc ttagggataa aaatatatcc
    27361 ctaaggtttg aatagcatag gggttgatgg tttttggaac atttattgga ggttaataat
    27421 ctatctgtaa gttttaaagt agaagaagga gaagttcagg ctgtaagaaa tgtatctttt
    27481 aatctaaaaa aaggtgagac attggctata gttggagaat ctggttgtgg gaaatctgta
    27541 ctgtgtaaga gtttgatgag gatactccca tacaatggat atattaaaaa tggagaggtt
    27601 ttacttaaat caagtgattt ggttaagaag tctgaaaaag agatggaaga tataagagga
    27661 aagaatatat caatgatatt tcaagaccct atgacatctc tcaatccaac tatatcaata
    27721 ggaaagcaaa ttgcagaagc tgtaatcatt catcaaggta taagtaaaag tgaggcaaaa
    27781 aagagagcca tagagcttat tgaattggtt ggaattgata atcctgagaa aagatttaaa
    27841 caatttccac atcatttttc aggtggaatg agacaacgta tagtaatagc tatagcactt
    27901 gcttgtaatc cagatgtatt gattgctgat gaacctacaa ctgctttaga tgtaactata
    27961 caagctcaaa taattgattt aataaaggat ttacaacata aaataggttt gtcaataata
    28021 tttataactc atgatttagg agtagttgca actatagctg atagaatagc agttatgtat
    28081 gcaggaaaga ttgtagaaat aggaactgta gaggatatat tttatgaccc tagacatcca
    28141 tatacttggg ggttgttggg ttcattacct actttagatt cgcaggatga ctatttatat
    28201 aatatacctg gaatgcctcc aaatttattg aacccaccaa aaggagatgc ttttgcaata
    28261 aggaataaaa atgcacttaa gatagattat gaaaaagagc caccaatgtt taaaattaat
    28321 gatacacata gtgcagcaac ttggttattg cacccagatg cacctgaagt agatgtacca
    28381 gttagagtaa attgtggaag ggtgatttct aatgaataga gaaaaaatac tggaaataaa
    28441 aaacttaaag caatattttc atttggacaa aagtactact gtaaaagcag ttgatgatat
    28501 aagttttgat atatataagg gtgagatatt tggattggta ggagagtcgg gttcaggaaa
    28561 atctacaact ggtaaaacta taataagact acatgaatct actggtggag aagtaattta
    28621 caaaggaaat tgcatatcag ataaaaaaac gtacaaattt ataaaaaaag atgtaaacaa
    28681 aagtatgcag attatttttc aagattctac ttcatcatta aatccacgta tgacaattgc
    28741 agatataatt tcagagccac taaaaattca aggtatctgt aaaaataaaa cagatagaat
    28801 gaataaagta tatgagatgc taaagctagt aggacttgat agaagttatg caaataagta
    28861 tccatctgat ttttcaggtg gacaaagaca gcgtattggc atagcacgag ctctttcagt
    28921 agacccagaa tttataatag ctgatgagcc tatagcttct ttggatgtgt ctatacaagc
    28981 acaaatagta aatttattta aaaaattgca acaagaaaag aatttaactt gtttatttat
    29041 agctcatgat ttatctatgg tgagacatat tagtgaccgt ataggagtta tgtataatgg
    29101 aaaattagta gaattggcag attcaaatga attgtataat aatcctattc atccatatac
    29161 aaaatcacta ctttctgcaa ttcctgttcc tgaccctaga tatgctaagt caagaaatag
    29221 aatagagtat aattctaatg gatatgattg ttcaaatgaa aagtcattaa gttggattga
    29281 tgtttcagat ggacattttg tatattcttc taaatcagaa ataaataaat atcaacaaaa
    29341 tttaaaagtt gtttagtata aaaatacttg aacaggtatt tatttgaaat acaaactaaa
    29401 accctatttt tggagaaatt ttaaaactac tccaaaatag ggttttttat ttctactttt
    29461 caaatataat aaattcaaat ataataaaat taaaaacaag taaagtatgt tgttaagaaa
    29521 gtactataaa attaaaaata gtaatgtaga aaaaatgagc aataatctta ttttttctac
    29581 aataattttc tataatttaa agtaaggaaa gtttgataaa ttataacctt agttaaagaa
    29641 taagagttta ttaaaaaata aagtgtgtat aggggtttta gttaataaaa tactataatg
    29701 taggggagct tgtaatgata tataaaaaag ataaagtaat gaaagtttta gaatcatcat
    29761 atagggaaaa tgaaaaaggg attacagcta accaattatc agaaaagaca ggaattgcaa
    29821 gaaacaatat aagtacatat ttaaaccaat tattcaatga gggtatggtt acgaagataa
    29881 aaggaagacc tgtgtattat atccctgcaa atcttaacct taacaagaat gaattgcttg
    29941 atacacaaag tgagttgctt ggcataaaaa aaggaacaaa agtagaaaac aaaataaaaa
    30001 aagatagttt tgaaacacta ataggtaaag aacatagttt aagacctata atagaaaaat
    30061 gcaaggtcgc aatgctctat cctacaaatg gacttcatac tattatctat ggagaaacag
    30121 gagtaggaaa gagtatgatt gctagatata tgtacgatta ttctatagat agtggaatta
    30181 gaaaaaataa agctcctttt gtaacattta attgtgctga ttatgctaat aatgcacaat
    30241 tacttatggg acatattttt ggggtagaac aaggagctta tacaggtgca gaatcttcca
    30301 gaaaaggtct tttggaaatt gcaaatggag ggatactatt tttagatgaa attcatagac
    30361 taccagcaga aggtcaagaa atgttgttta cttttataga taagggaaca tttaaaagac
    30421 taggagatgc aacgaaagaa agagagtcta aggtattaat aatatgtgct actacagaaa
    30481 atccgacatc aacactatta gatacattca atagaagaat accaatgaaa atagaaatac
    30541 catcactgag agataggaca atgattgaaa gaatggaatt agtcaaaaat tttttcaagg
    30601 aagaatcaaa aagtatttct atttcaataa aagtacataa agaaataatt aaatcactat
    30661 tactttatga ttgtaaaaat aatgtaggtc aacttcaaaa tgatataaaa ttagctgttg
    30721 caaatggata cctaagatat aaaagagata ataaagaacc agtttatata gagaaaaaat
    30781 attttgataa atctataaat gaagtgagtg atgattatag agagaagtct attaaagcag
    30841 atgaagttat aacacatgga atagaatatt ttgtatttac gtcattagga gaagaagaag
    30901 ttattaacta caatgatata aatattatta atgcagtaaa tactaatctt aataaatctg
    30961 tagaaatgaa tatagcagat gcttctctat ttaataatgt taagtttaaa agcatgtgtg
    31021 aatcaataag acaaattatt aaagaagatg aagaaataga cataagtgaa tcaatattta
    31081 agtcaatggc aatttatata aaaagcgtat tggataaaag tagctctaca aacaacatag
    31141 atttgaatca aattagaaga aataacagaa gtgaatttaa aacagcacta aaaatagttg
    31201 gaataattga aaatgaattt gatttatttt tatctataga aactgtagca tatattacat
    31261 tattcatagt acaagctaaa caagatgaac aaaataaaat agtgaataac attaatatta
    31321 tagttgctat gcatggagaa actacagcaa gtagtatggt aaaagcagtt ggtgatatta
    31381 ttggaaagtg cagtgctatt tctttcgata tgaagttaga taaaagctat gatgaagtta
    31441 ttgtagattt taaaaattta attaataata tagacaataa agatgtatta ctatttacag
    31501 atatgggttc attaaactct tttgatgaaa ttataaagaa agaaaaaaag tgtggagtaa
    31561 gagtaattcc tatggtgaca acattaactg tgttagaagc agttcagaag gccaatatgg
    31621 gattaccttt aaatgacgta tacaactcta taactaacac gagaaagtac tattttggaa
    31681 ctaatgaaat tcaaaataaa gaaaatctgt ctaaaacaat tattatagca tcacatgttt
    31741 cagaaggtgt agataataaa acaagaaaaa tattagaaga aaagatgagt aggtacttag
    31801 atggaataga tattatatct gtcccatata aaacagaaaa agatttaagt ttaaatatta
    31861 cgaaacttaa agaaagtagt aatattgttg cagtaatcaa tgaacaaaga attaatatta
    31921 gaggaattga ctatattagc aaaaaagata tagataagga tgaaaatatc aataaattaa
    31981 aaaatattat aaagataagt ataggttatg atgatgtagt tgaaggttta aagacatcct
    32041 taaaaagctc taattataat aggatattta aagatataaa atatgtgtct gatgaattgt
    32101 ttttagtatt taatatagaa aagaaatacg ataaagtaat tggacttatg atgcatttag
    32161 ctttcatggt agatggattg attggaaata ctagagaaat tgaaaagctt gataaagaaa
    32221 aaacacttga ttatcacaag agcctaagca aaataaaaga tatagtatct caattagata
    32281 aaaaatataa tatagaaatt aatgaaaaag agtgctatca aatattatta atattagaat
    32341 atgcagaaat aatcgaaaaa gattaccaat gagtataaaa acttatttgg taattttttt
    32401 tagtctaaaa taatattaat attagacaca tataaatata cttagacaaa gtatctaagt
    32461 tttgatgata aaaatgattg aaataacagt gttttaaagt gtctaaaaaa actggcataa
    32521 tacttgctta tattattaaa tgagtaatat aaataattaa aatttaaata aaaaggagta
    32581 aataatatgc aagataaaat attaagagat aaaaaggcaa atgacccatg ggcaagaatg
    32641 actacaagaa acggatttag tgcagatgaa ataatatcgt catttcaaaa agctataaga
    32701 agaaatatgg tggaagaagc ttgtgaattt gcttatgaac tatatatatc atcaccagaa
    32761 ttagaagata agctatggag aaggatactt acaatatcag ttgaagatat aggaatgggg
    32821 gacccatcag ctgtaataat cataaataat ttaaatcaag taagaaagga atattcatat
    32881 gcagatggag acagaccttt atttttcata catgcaataa gatatctttg tgcatgtgag
    32941 aaagatagat caagtgattt attaaaaaat ataattataa aaggatttgc aatgggtaaa
    33001 gttccagaag ttatggacgt ggcacttgac aagcatacat atagaggatt agaaatggga
    33061 agggattcat tccacttttt aaatgaagct agtatagtaa taccagaaaa agaagtagat
    33121 aatgattata aagaaagata cttaaaaata ttagaaaaat ataagcaaga agaagttata
    33181 gatacggctt tcaagtttaa tcattggcag tactaagaat tagagtaaat attaataaga
    33241 attaaaataa aataataaga attaaagtaa aaactgatag gaattagagt aagaattggc
    33301 tgataaaaaa ttagaaaata aaagaggtta gatattgtag gagggtaatt atgaatattt
    33361 tattagtatg tgcaaatgga gcttcaacag gtgtacttgt ggaaaagatg aaaagttttt
    33421 gtagtgagca tgaaaagtta aaaactaaaa ctataaatat agaggcaaca tcatttgaaa
    33481 atttaaaatc atacattgaa gctaatgata ccgatgtagt acttgtagca ccacaaatta
    33541 ggtttaagga agatgatgta gttgaagctt gtaagaatta caaaatagca gttggtttaa
    33601 tagatactaa gcattatggt cgtatggatg ctccttctgt tatgaagtcg gcaatcgaat
    33661 tatataaaaa tagataattg gggggaataa aaatggaagc acttcaaaat aatttaagaa
    33721 gatttcttct tccaatagca caaaagattg aaaaacaaag gcatcttcaa gctataaaag
    33781 aaggtatgat aagcattacc ccaataataa ttgttggttc tttaagtatg ttatttatgg
    33841 cattaaacaa tatgttacct gaaggaagtg caaaaacatt attatctgaa aatatggata
    33901 cattacttat ccctaataaa tttactatga gtttattatc tatatattca gcatttttta
    33961 tagctcaggc actagctaaa aaatataatc ttaatcatgt agaaataggt atgacagcag
    34021 ttgttgcaca acttgttgta tgtggacaag tagtagatgg tgtattagat acaagttatt
    34081 tagatgcaca aggtttattt gtaagtatat tagtagcact tttagtagtt gatattacta
    34141 agtttatgaa tgataaaaat ctcgtaatta gatttccaaa agaagtacct agtgtagtaa
    34201 ataaaagttt tagaaactta actccaatga tagtatgtat aatgttattt acagcaatag
    34261 cagcaataac taaaaatgtc tcaggtcaac cattaccagc tattataatg aatttcttag
    34321 caccagctat aagcagtgta gataatgttt ttgcagtaac tataatacta tttataactc
    34381 aattattatg gttctttggg cttcatggag cagcaataac ttcaagtata tggatgccaa
    34441 tagcagctac atatatggca gaaaatgcaa cgttaatagc agcaggagga gacccaaaat
    34501 atgtgtttac aataggtttt tactatggtt tcttgcaagt aactggttca gggataactt
    34561 tgggattagt gtaccttatg tcaagaagta agtgtaagag ttttaatagt atgggaaaag
    34621 ttgtgatttt accatcacta tttggaataa atgagccggt aatatttgga acaccaatag
    34681 ttatgaaccc atatatgttt gtaccatttg tatttggacc tgtattggtt ggagcactta
    34741 attttatggc attaaaagta ggattagtag gattacctat tgcagaacca ccaggctttt
    34801 tacctccagg ggttgcagca tttttgatga cattagattg gaaggcaatc gtattagtat
    34861 ttgcaagtat aatacttatg accttgatat attatccatt ctttaagata atggagaagg
    34921 aagaattaaa taaaaatgca gaattagcta caacattaga tgatgatagc tttgattttt
    34981 aacaagtcat tagaaggaga aggatatggt acctgaaatg gaagaaaaag tatttagtat
    35041 aatatcagaa gcaggagatg ctaaaagtga tgtaatgatg tctcttaagg aaataaaaaa
    35101 aggtgactac aataaagcaa aacacctttt aaattcagcc tcagagaaaa tacaaacagc
    35161 atcaaagcat catttagaat tactatcaaa tgctatgaat agtgaagata gtgggactga
    35221 ttttttagtt gtgcactctg aagaccatta ttcaaatgca ttatttgctc attctttagt
    35281 atctgaactt gtagatatat ttgagatgat ggacccaaga attaataaga attaaatttc
    35341 ttgtaaatag tgcttgaagt ttggctatag tttattaaat caatagattt aataaatgaa
    35401 ctccctttta ctttaaagta ctagtattta taaatttatt tataaataca gtaaattttt
    35461 aaatagaaaa ggagatatta ttatggaaaa attttcagta gtaatagctg gaggaggaag
    35521 tacatatacc ccagaaatca tattaatgtt gctagataac ttagataggt taccattaag
    35581 agcaatcaaa ttatatgata atgatgaaga aagacaaaat aaggtagcta aagcttgtga
    35641 aatactaata aaggaaaagg accctaatat agagtattta gcaacaacat gccctaaaga
    35701 ggcttataca gatgtagatt tttgtttagc tcatataaga gttggaaaac ttgaaatgag
    35761 agaactagat gaaaagatac ctctaaaaca tggggtagtt ggtcaagaaa cttgtggacc
    35821 aggtggaata gcttatggaa tgaggtctat agaaggtgtt cttgaaaaca tagattatat
    35881 ggaaaagtat tctccaaatt gttggatgtt aaattattct aatccagcat caataatagc
    35941 ggaagctgtt agaaggttga gacctaattc tagggtaata aatatttgtg atatgccaat
    36001 aggtatggaa cataatatag caaggattgc aggactcaaa tctcgtaaac atatggatat
    36061 aagatacttt ggattaaacc actttggttg gtatacttca attaaagata aaaaaggaaa
    36121 tgaactttta ccaaaattag tagaacatat aaaagaatat ggttttataa atggtgaaga
    36181 aggaatgaaa aatgacaaga aagatagttg gtttgaaact aatttattta ctaaagaaat
    36241 agtaaaaaca gatttaacta caataccaag tagttactta aagtactatc tatttccaga
    36301 ttatgttgta aatcattctg atgtaaatta tactcgtgcc aatgaagtta ttgatggaag
    36361 agaaaaagaa gtttttggtt catgtgcatt gatagaaaag caaggacatt ctaagggcag
    36421 tgggcttaag ataggaatac atgcagagtt tatagtggat ttagctacag ctctagcttt
    36481 taatacacag gagcgtatgt tattgatagt tcaaaataat ggagctatag aaaatataca
    36541 agatgatgct atggttgaaa ttccatgtat agttggaaaa gacgggtatg aaccattatc
    36601 aatagggaaa ataccaacat tccaaaaagg tttaatagaa caacaattag cagttgaaaa
    36661 attagtagtt gatgcttgga ttgagaagtc ttataataaa ttgtggcaag cacttatgct
    36721 atctaagaca gttcctagtg caacagttgc aaaagaatta ctagatgatt taatagaagc
    36781 aaataaaaat tattggccaa aacttaatta agaataatat atttttaaag ttcctacagt
    36841 ttagtacata gcctagttaa tgtatttgta gggactttat ttatttacaa atgagataca
    36901 agtggcaaac atagtgtatt ttgcagttag gaaagtaggt ttagttactt gttagttgga
    36961 ttaaatttag gtacaataat ttactatcaa tcaatagaaa taaccagaga agttgcataa
    37021 taaaaaattt acacttttaa caggatttat gtaggtgttt atagaaataa ataaatatgt
    37081 acataaatac ataattattt ttaagtctaa aatacaagaa tggagagatg gctatgaagt
    37141 ggagagatta tgctgatgat gtaaatttat ttgaggattt tgataaaact atcaagcctc
    37201 taactgacga acaaagaaga aaaaatataa acactttaat tgcatatttt tcaaaagaag
    37261 ttccttctaa aatatacaat ttatcaaatg atgaaattaa gcctagagat atacttagag
    37321 gacttttgaa tgtatatcca ccaaaagaaa ttgctccaga gatacttaat atgttacata
    37381 atttattgtt gattgaatgt gaagaaagag agttagtaga tgtaaatgat atagaagaaa
    37441 tagaggaagg gattgccata tggagaggta atataacgaa tttaagagca gatgcaatag
    37501 taaatgctgc aaataacaag ttattagggt gtttacaacc attacatcta tgcgtggata
    37561 atgagataca ttcatgcgca ggtcctagac ttagagaaga ttgtgacaaa ataataaaaa
    37621 aacaagggca tttagaatat actggagatg ctaagataac tagaggttat tgtttacctg
    37681 caaaatttgt agtacatact gttggaccta ttgtatctgg tggtcagcca agtaaagagc
    37741 aagaaaagca actgcttcat tgttataaat catgtttaaa tacaataaaa gaaattgatg
    37801 aaataaaaaa tattgttttt tgtggaattt ctacaggtgt atttggttat ccaaaaaaag
    37861 aggcagctaa tcttgctgta agtagagtta gactatggct aaaagaaaat cctgaaaaaa
    37921 atttaaaagt tgtatttaat gtgtttactg aagaagaaga agagaaatat agaagaatat
    37981 ttaaataata ttatgtaaca gaatcctaaa aatacttatt atataataca aattcattgt
    38041 ataagataat aatttacaag tagtaaaaat taataaaata taatttgtat ttttaataaa
    38101 taagtaggta taatagaaat tataagaata gtataatcaa ttatgctatt acttagaaaa
    38161 tataatatag gaaaattgta tggtaaataa atatgaaaaa tagttttaaa aattgtatta
    38221 gtttaagaaa taaatttata attatatcaa ttgtgtttct attaatccta ttaattgtat
    38281 cttatatatt tggcaaatta gtgtataata gttcagttgg tttagagcag ttaataaaaa
    38341 acgaagatat agtaaaaata ttttctgaga gaaaagatag accactggag aaattaaaaa
    38401 actacaagac atctgaatta atgatacaaa gttctaataa ttacaagctt gaaagtcttt
    38461 ttataacttc aaatataaag acaagagata ctatgatact tgttcatgga attggaagta
    38521 gttactatga aatgttaaag gtagcatata gatatttaga taaaggttat aacgtattag
    38581 tttataatca aagaaatact ggtaatagtg gtggagataa ttacacattt ggtctttatg
    38641 aaaggtatga tttagatagt ctagttaaat ttgtaaaaaa taaatttcct gaaggaagat
    38701 taggagtgca tggattttcc atgggggcag ggacagctgc tatgcattca gagataaatt
    38761 caaaagatga taaggtcgat ttttatatac tggattctcc ttattctgaa atgaaagatg
    38821 caattagaat gggagtacta gaaaaacgaa taccagatat attaatcaat tatgtggtga
    38881 cttgtggaga tttatataat aagtttaaat ctggattttg gtacagtgat gtaaaaccat
    38941 atgagtcagt tgagaaatct aatgtaccca tactgtttat acatggaaca aaagatactg
    39001 tttgtaatta tcaaaatagc aagaaaatgt atgatttagt taaacatgat aaaaaagacc
    39061 tatggcttat agaaggtatt ggtcatgttg atggatttga acatgatagt acagtatact
    39121 tcaataaaat atttaaattt atagatagta atgttttaag tgataaaagt taattgaata
    39181 ttagtagctt atttattttt gtatttatta aaaaatagat aggctatttt tatgtttaaa
    39241 tataagtttt gtttagtaat gtgaattaat ttctagtaaa aatgaaaata actaccaatt
    39301 aaattcattt atacctcaaa taaaagagtt agtattagat tacaatcaaa tctaaagaaa
    39361 agagaggtag tatatatgaa aaacaaaaat aataaagata aaaacatgtt gaatattaaa
    39421 gtattgagag ttctaggagt gctattaata ctttttgatt atacgggtga tactttgaat
    39481 ttttataatt tcttcgcaaa gccattagta tcgggtaatt tatataagac aacaggtact
    39541 gactgcattg tatttttatt atttattgta gagtgtgtat acctagtggg aagatatata
    39601 ttaaaagtta agataaattg gagtgagatt attgaaaatg agaaaggtaa ttttataaat
    39661 aacttgttgg ttgaaaaaat acaagaagaa agcaacattg tatataattt attaaactca
    39721 tatgtgttgc caaatatata taagaaagca aggggattga tgtttaaaac atatcatatg
    39781 ttaatctaca ttaaagaaaa
//
LOCUS       DAYAOY010000030        39466 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYAOY010000030 DAYAOY010000000
VERSION     DAYAOY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.030"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            469..4014
                     /locus_tag="ACGI6B_001631"
     CDS             469..4014
                     /locus_tag="ACGI6B_001631"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9751078.1"
                     /translation="MNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHTY
                     LSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMT
                     ISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSG
                     KIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLF
                     DKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIV
                     SIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGV
                     KTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLE
                     KQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKR
                     FEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKD
                     DRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDI
                     TSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENAT
                     KNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNN
                     GKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGA
                     LMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDIS
                     ETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALYS
                     AKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEV
                     AVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGN
                     YLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYN
                     RMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYE
                     LLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFE
                     VQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4199..6133
                     /locus_tag="ACGI6B_001632"
     CDS             4199..6133
                     /locus_tag="ACGI6B_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFG9751079.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6239..7234
                     /locus_tag="ACGI6B_001633"
     CDS             6239..7234
                     /locus_tag="ACGI6B_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HFG9751080.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7206..7991
                     /locus_tag="ACGI6B_001634"
     CDS             7206..7991
                     /locus_tag="ACGI6B_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751081.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7979..8677
                     /locus_tag="ACGI6B_001635"
     CDS             7979..8677
                     /locus_tag="ACGI6B_001635"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751082.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8800..9273
                     /gene="ybaK"
                     /locus_tag="ACGI6B_001636"
     CDS             8800..9273
                     /gene="ybaK"
                     /locus_tag="ACGI6B_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFG9751083.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9470..10309
                     /locus_tag="ACGI6B_001637"
     CDS             9470..10309
                     /locus_tag="ACGI6B_001637"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFG9751084.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10322..10870
                     /locus_tag="ACGI6B_001638"
     CDS             10322..10870
                     /locus_tag="ACGI6B_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HFG9751085.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10936..12117
                     /locus_tag="ACGI6B_001639"
     CDS             10936..12117
                     /locus_tag="ACGI6B_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HFG9751086.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12394..12798
                     /locus_tag="ACGI6B_001640"
     CDS             12394..12798
                     /locus_tag="ACGI6B_001640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9751087.1"
                     /translation="MEVQEMMEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSK
                     TLNITAYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTML
                     YFGAQPGVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12948..13211
                     /locus_tag="ACGI6B_001641"
     CDS             12948..13211
                     /locus_tag="ACGI6B_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9751088.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13340..14182)
                     /locus_tag="ACGI6B_001642"
     CDS             complement(13340..14182)
                     /locus_tag="ACGI6B_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HFG9751089.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14477..15211
                     /locus_tag="ACGI6B_001643"
     CDS             14477..15211
                     /locus_tag="ACGI6B_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9751090.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15221..16354
                     /gene="nagA"
                     /locus_tag="ACGI6B_001644"
     CDS             15221..16354
                     /gene="nagA"
                     /locus_tag="ACGI6B_001644"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFG9751091.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16453..17202
                     /gene="nagB"
                     /locus_tag="ACGI6B_001645"
     CDS             16453..17202
                     /gene="nagB"
                     /locus_tag="ACGI6B_001645"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFG9751092.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17377..17649
                     /locus_tag="ACGI6B_001646"
     CDS             17377..17649
                     /locus_tag="ACGI6B_001646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751093.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18309..19013
                     /locus_tag="ACGI6B_001647"
     CDS             18309..19013
                     /locus_tag="ACGI6B_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751094.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            19015..20502
                     /locus_tag="ACGI6B_001648"
     CDS             19015..20502
                     /locus_tag="ACGI6B_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9751095.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFVKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20707..22320
                     /locus_tag="ACGI6B_001649"
     CDS             20707..22320
                     /locus_tag="ACGI6B_001649"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9751096.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22683..23513)
                     /locus_tag="ACGI6B_001650"
     CDS             complement(22683..23513)
                     /locus_tag="ACGI6B_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9751097.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23586..25313
                     /locus_tag="ACGI6B_001651"
     CDS             23586..25313
                     /locus_tag="ACGI6B_001651"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751098.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25350..27212
                     /locus_tag="ACGI6B_001652"
     CDS             25350..27212
                     /locus_tag="ACGI6B_001652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751099.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27545..28234
                     /locus_tag="ACGI6B_001653"
     CDS             27545..28234
                     /locus_tag="ACGI6B_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HFG9751100.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28351..29025
                     /locus_tag="ACGI6B_001654"
     CDS             28351..29025
                     /locus_tag="ACGI6B_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFG9751101.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            29042..30949
                     /locus_tag="ACGI6B_001655"
     CDS             29042..30949
                     /locus_tag="ACGI6B_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HFG9751102.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31402..32091
                     /locus_tag="ACGI6B_001656"
     CDS             31402..32091
                     /locus_tag="ACGI6B_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9751103.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32527..33066
                     /locus_tag="ACGI6B_001657"
     CDS             32527..33066
                     /locus_tag="ACGI6B_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9751104.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            33085..34128
                     /gene="potA"
                     /locus_tag="ACGI6B_001658"
     CDS             33085..34128
                     /gene="potA"
                     /locus_tag="ACGI6B_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9751105.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            34130..34966
                     /locus_tag="ACGI6B_001659"
     CDS             34130..34966
                     /locus_tag="ACGI6B_001659"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751106.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            35071..35745
                     /locus_tag="ACGI6B_001660"
     CDS             35071..35745
                     /locus_tag="ACGI6B_001660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751107.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35745..36797
                     /locus_tag="ACGI6B_001661"
     CDS             35745..36797
                     /locus_tag="ACGI6B_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751108.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            37027..37875
                     /locus_tag="ACGI6B_001662"
     CDS             37027..37875
                     /locus_tag="ACGI6B_001662"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9751109.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37865..>39466
                     /locus_tag="ACGI6B_001663"
     CDS             37865..>39466
                     /locus_tag="ACGI6B_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HFG9751110.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISV"
BASE COUNT        15623 a         4045 c         6877 g        12921 t
ORIGIN      
        1 ttataatatt ttactagaat tattatctgg taacttaagt tttgagtatt aatctaaagt
       61 atatgttttt atatggttta aattttatgt atacaaaatt aatttgctaa ctgaatcata
      121 taaaaaaaaa caaaatattt tttagaggga atgattagat agaatttttt tgttatgaaa
      181 ttaattgaag agtaattaat tagaaaagga atgaataata atgaatatat tacattatta
      241 ctttttgata aatcaaaaat aaagttataa tttttaatat taattaagaa tgatgaaata
      301 tgaataaaga ttttacaaat aaatcttaag ctatcttatt taattctaat acaggaagaa
      361 ggtaaatgta tattgaaaaa atcatagaaa tagaagtaaa caatttaaat aaatacaaag
      421 gtttgcaaaa gaagtaatat aattatatac aatgaggatg tgagtatgat gaacagacta
      481 aagccaagaa ttttaggtat tagtatcttg tttattatta taattatttt aacatgtgga
      541 gctgttttca agtttaaatt taaattagat aataaattaa aagaaaccac acatacatat
      601 ttgtctgagt ttacagaaca caatataatt aatctcaaga caagacttag tggtcaattt
      661 gacatgcttg aatctattgc taacttcata ggaaacttga ataatataaa tgatgaaatg
      721 gtgattgatt taatggattc tgatgtaaag cgaagttctt taattagaat gactatctct
      781 acattagatg gggaatgtta ttctaatgat ggaattaccc ataatgttca agatagagat
      841 tattttaaaa aagctaaaaa tggagaaaag aatatatcag agcctttaaa atctattgtt
      901 aataataaag aaataatagt tttatctacc ccagtttata aaagtgggaa aatagttggt
      961 gttttaagtg gaatttatga ttcaaaaaag ttaaatgagt tattaggatt atctgcattt
     1021 gatgaaaaag cagatgtttt tgttgcaaag aaaaatggag atatagttgc tcaaaaaaca
     1081 aataaaaata gaaagacgct atcatgtata tttcagcttt ttgacaaaga taattgtgat
     1141 gaagatgatt gtctaaaaac tataaaagaa aatatgaaaa atgataagtc tggatatttt
     1201 aattataact tagataatga aaaatttatg ttaaactatc agccacttgg agttaatgat
     1261 tggtatgttt tttctgttgt gccaaataaa atagtatcta ttcagtcaga gaatatatct
     1321 tatgatgtat atatttttgc attagaaata ctactagtta tattgatttt tataatatat
     1381 atagcatgta ttataaataa taacaccaaa attattataa atgaaaaaga aaaatttaaa
     1441 gtactcactg ataatatatt aggaggggtt aaaacctgtt taaaagatga atatatgact
     1501 atgacatatg taagtcaggg gtttttagag cttacaggtt actcttgtga agatttaaat
     1561 aaaatattta aaaatagatt taaagacatg atttataaag atgatttaga caagtttaat
     1621 tgtattattc taaatcaatt agaaaaacaa gataaaattg aagttgaata tagaattgta
     1681 agaaaagatg gaagtataat ttggattttg gagagaagta agtctataaa tgataataaa
     1741 ggaagtgagt gtattcaggg agttttaact gatataacag aacttaagaa aatacaagaa
     1801 gaattagatt caaaaagatt tgaaatacag gctatcaatg atagtattgt tggtggaata
     1861 gtgataactg agataaatga tgattttgat agtatatatt taaatgaagg ttatttaaat
     1921 atgattggat atacacgcaa acaactggaa gatgagctaa ataataaatt aatcaaactt
     1981 atttttaaag atgatagaac agaattaatt aatagtataa acaaacaact caaattaagt
     2041 gatacatttg catctgaaca aagagttgaa aaaagggatg gcactattat atggatttta
     2101 ttaaaaggaa gaaagatgat ttatgataat ggaaaatctg tactttgttg tgttattgta
     2161 gatattactt ctgctaaatt attagagcaa gaacttaaaa agagtgaaga acgtcatgaa
     2221 atcattctaa agcagacgca aaatattata atagaatggg atatagaaaa agataaaatg
     2281 aatatatcac ctaattggta taataagttt aaatatgacc ctataactga aaacgcaaca
     2341 aaaaatataa ttaaatcagt agatatatat catgatgata tggataagtt tatttcatta
     2401 attaaactta cttcacaagg tagtgagtat gaagagtgtg aaattagact tagaactaga
     2461 gatactattt acgcttggtt tagtataaag gcaacaactt tatttgataa taatggtaaa
     2521 ccttataaag tagtaggaag tttgactgat attgatgaat caaaacgaga agctgaaaag
     2581 ttgaaagaaa gggcagaaaa agaccctctt acagatctgt ataataaaaa agcatcacaa
     2641 tcacttatag aggagtacat aacattggat aaaaaaagaa aaggtgcttt aatgattatt
     2701 gatattgatg actttaaagg aataaatgat aatttaggac atttatatgg ggatgctgta
     2761 ttaagtgaaa ttgcaaatga cctaataaat ctatttagag caaccgatat agtaggcaga
     2821 attggtggag atgagtttat tgtatttata aaagatattt ctgaaacaag agatattata
     2881 aataaagcag aagaattggt aaaaacattt gaaaggtctt ttttaggtaa aggacgaaat
     2941 tataaaatat cattaagtgt aggaattgct aagtttccac aagatggtat ggactatatg
     3001 acactattta gaaatgcgga tatagctctt tactcagcca aagggaaggg caagaattgt
     3061 tatacattgt atgataaaaa atttgataaa gtccaatata taactaatac aagaaatgca
     3121 ggagatagtg ataatgtaga gatgtctttt aaagaacata tcacaaatta tatatttgat
     3181 atactatatg aatcaagtga tatagaagtg gcagtaaatt taattctaag tattgtaggt
     3241 aaacacttta atgtgagtcg catgtatata tttgaaaatt cagatgataa tcgagtatgt
     3301 aaaaatacat ttgaatggtg taacgaaggt gtaacctcgg aaattaataa tcttaaaaat
     3361 atttgttaca aggatttagg caactatctt ttaaatttta atgcagatgg gttattttat
     3421 tgtaatgata ttgaaaattt aggtagtgat gtgaaaaaag tacttaagcc acaaggcata
     3481 cgttcaatat tacaatgtaa aattttagag ggtaatgaat ttagagggtt cataggattt
     3541 gatgaatgtt catataatcg tatgtggact aatgatgaag tgaacacttt gacatttata
     3601 tctaaaacgc tcagtatgtt tttattaaaa atgcgtattc agagtaagct agaagagtct
     3661 ttaacaattc aaaaatctgt gatggataat atggatatat gggcatatgt tgttgacagg
     3721 aatacctatg aattactatt tatcaataaa aaaatgcttg agtcagcacc aaacactcat
     3781 gtaggtgatt tatgttataa agcgatatgg aataatcaag ataaaccttg taaaaaatgc
     3841 cctatgatat atttaaaaga tggtgatgat acttataaat cattcatgca taataaaaag
     3901 tttgaggtac aggcaaatat aactacaact actttgaaat ggtttgatgg taaagatgca
     3961 tgtttaatat ttggaagtga aataactagt attgaagctg aagaaaatac ttaaaatact
     4021 gtgttatata tatacttaaa atcgcaattt gaatttatta gacttaaaat gtctatataa
     4081 atcatttaag ttgtggtttt aagtattttt tatgatataa ttaagcgtta agaacaaaat
     4141 taaaagatac ttttgtataa aaaaatattt gttaaatata tattgagcag gtgataatat
     4201 ggaaaataag tttttaccaa taagcaaaca agatatgata gatagaggat gggaagaatt
     4261 agattttgtt ctagttacag gagatgctta tgtggaccat catagttttg gaacagcaat
     4321 aatatctaga gttttagaaa gtgctgggta taaagttgga ataatagctc aaccagactg
     4381 gagaactaca gatgatttta tgaagctagg aaaacctaga cttgcatttc tagttaatgc
     4441 aggaaatatg gattcaatgg tcaatcatta ttctgtaagt aaaaagcata gagaaaagga
     4501 tatgtattct ccaggtggga aaatgggtta tagaccagat agagctacta tagtatattg
     4561 caataaaata agagaagcat acagtgatgt tgcaatagta ataggtggaa ttgaagcgag
     4621 cttaagaaga tttgcacact atgattattg gagtgataag gttagaaagt ctatgttaat
     4681 agatagtgga gcagatttat tagtttatgg tatgagtgaa aagcagattg tagaagtagc
     4741 caatgcactt aatgatggat atgacccaaa atacattaga catattgatg ggacatgcta
     4801 tatttcagat acattggaag aaatatatga taaatatata ttaattccat catataaaga
     4861 aatttgtgaa gataaaatga agtatgtaga agcttttaaa attcaatatg atgagcaaga
     4921 cccatttaga ggtaatataa ttgttcaacc tcatggaagt aagtatctgg ttcaaaataa
     4981 gccagaaaaa ccacttagta gagaagaatt agatgaagta tatggattac catatcagaa
     5041 gacataccat cctgtatatg aaaaatttgg aggaatacca gctatagaag aagtaaaatt
     5101 tagtatagta agttcaagag gctgttttgg tagttgttca ttttgtgcaa taacatttca
     5161 tcaaggtaga gcagtgcaaa gtagaagtga aaaatcaatt attgatgaag caataggaat
     5221 aacaaattta gatgatttta aagggtatat acatgatgtt ggaggtccaa cagctaattt
     5281 tagaagacca gcatgtaaaa aacaaattac taaaggtgct tgtaaaaata gacaatgctt
     5341 atctccatca ccatgtaaaa atttagatgc agaccacagt gaatacttac atttacttag
     5401 agctgtaaga aaattaccta aaataaaaaa ggtgtttgtg cgttctggaa ttagatacga
     5461 ttatgtaatg gcagataaga acaataaatt tcttagagag ttgatagagc atcatgtaag
     5521 tggtcagtta aaggtagccc cagaacatat atcagaagaa gttttaaagt atatgcaaaa
     5581 accagcagga aaaacttatg ataaatttag acagaagttt tttgctataa atgaagaatt
     5641 aggtaaaaag caatatttga taccctattt aatgtctagt caccctggtt ctaccttaaa
     5701 tagcgctata gagttagcag agtatttaag agatactcac taccaaccag agcaggttca
     5761 ggatttttat ccaactccag ggactctatc tacaaccatg ttttatacag gtattgaccc
     5821 gcttacaatg aagccagttt atgttcctaa atcaaaacgt gacaaggcaa tgcaaagggc
     5881 tttattacaa tatagagcac ctagaaatta tgatttggta tactctgcgt tagttgaagc
     5941 aggaagagaa gacttaattg gttttggaca tagatgtctt ataaagccaa aaaatgaaaa
     6001 gccttatttt aatagaaata attctaaaaa aaatgtatct aaaggaacaa ataaaaacaa
     6061 aaaaactaat acaaataaca gaagtaataa aaatcaacaa aaaagttcaa ctaaaaaaaa
     6121 gaaaaaacat taaagtataa ataaaaatac ttaaaaaaga aggattattc gataaattaa
     6181 cagaaattag tataaataaa tataattatt ttaatgacgt tacctttggg aggaagagat
     6241 gaaaaagtat atttctataa tattattagt ggtgttgact atggttgttt tggtaggatg
     6301 ttcaccagga aaagataacc ctaaaaataa ggagctatca gttgtcaaac aagtaaaagt
     6361 agctgttcca gatggactac cagcagtggc aatagctaaa cttgcaaatg aaaatccaga
     6421 gattaaagaa ggatatgaaa cagtgtatag cattgaaaag acacctgaag cgatttccac
     6481 tagagtgatg aaaaaagatg cagatatagc tatagttcct tcaaacatgg ctgcaatagc
     6541 gtataataaa acttcatcat ataatattgt tggaactgta ggaatgggtt cactttattt
     6601 ggtgtcaaca gaaaatataa aagattactc tgatttagtt ggtaaagaag ttggatgtac
     6661 tggaaaagga cttactccag acataactat aaaaagtctt ttgttacaaa aggatataaa
     6721 ttattcagat ataaaattta attatgtaaa ttcagcaagt gaactggtac cattattggc
     6781 tactggtaaa gttaaaacag gtattgttcc agaacctgca cttagtgctt tgatgtctaa
     6841 aaatccagac ataaaaatta taaaaagttt aaatgactca tggaaggaag taagtggttc
     6901 aaaagatggt tatcctcaat ctactttaat tgtaaagtct gaatttttaa gagataataa
     6961 agattttgtt gattcttttg taggtcaatt atcaaatagt atagattggg caaacaaaaa
     7021 tcctgaagaa ttaggtgcat attctgaaaa aataaaaata tctactgaat caaagataat
     7081 aggaaaatct cttgaaagag ctaatttgaa atatattcca gttaaggaca tgattaaaga
     7141 ttataaaaat tactatgaga aattagctaa ttttgatgat aagacattag gaggcaaagt
     7201 accagatgaa gcaatatact ttgttgagaa ataaaaaaaa taaagaacaa tttatagata
     7261 aaatggaggt aacactttct tgtgttatcc tcttattttt atggcaaatt attgctttaa
     7321 aaattgataa tgatattttt ttaccaacag taggtcaagt gtttaatagt ataaaagaga
     7381 ttatgctaaa ttctagtttc tatatagata tactttactc tatggggaga tgttttttta
     7441 gttttttact ggctatgatt tttgctatta tactttcaat agtgtcttat ttaaatagat
     7501 tttttagaaa cttattaaag ccaatcaatg ctttgacacg ttcaatacca actatgatat
     7561 tggtagtact tgcacttatt tggtttgaaa aggatagtac cccttttata gttggattta
     7621 ctatagtttt tcctatattg tatgataacg ttttgggcgc tatattgaat atagacaaga
     7681 acttattaga aatggctaat gtgtataaaa taaggtttat agataaggtg ttaaaaattt
     7741 atttaccagc cataaaattt caaattatat ctatactagt atcaacattt tctttaagtt
     7801 tgaaagttgc tatagcagga gaggtgtata gtcaacctac atatggtatt ggaactatga
     7861 tacagactga aaaaattaac tttaacacat ctggaatatt tgcatggata attattgttc
     7921 ttttaatatc tgctatttta caaatagctc aaaagttttt agcaaggaga gcttttttat
     7981 ggaaaagata aagttgagta ttgaaaatat aaataaaaga tatgatagtc gtattatatt
     8041 tagagatttt aatattgatt tttatgtaaa tgaggtaaat tgtatccttg gaaaatctgg
     8101 ttgtggaaaa actacactct tgaacataat aagtgggatt attaaaaatg acaccaacaa
     8161 tctaaatatc aaagaaaatt taaatagagt aggaaataag cttgaagcta gttacatatt
     8221 tcaagatgat agacttatag actggttaac agtagaagaa aatataaaga tagttgtaaa
     8281 taaatactat aataaaactc aattaaataa aatttgtgat gaatacttgg aattggttgg
     8341 aattagtgat tataaaaaat tctatcctca aatgcttagt ggaggtatta gacaaagagt
     8401 aaatattgct agagctttta tatatccatc aaaaaacatt ataatggatg aaccttttaa
     8461 atctattgat gcaaagaata cacagcttat aatggataat tttagaaata tattaagaaa
     8521 agaaaagaga actgtgttat ttgtaactca taatattgag gaagcattgt ttttagcaga
     8581 taggatattt attttaggag actctccaat tagaataaaa aaaatattaa aaaattctaa
     8641 ggaattggaa aaaaatgaag ttttgaagtt gatatagagt tataataata ctgtcgtttt
     8701 aaaatatatt tggttaaaat ctataagact atattaaaat agtacagaat attcaaaact
     8761 gtgtataatg ttcgtatttt ataaaaagga agtgaatgta tggttaagac taatgctatg
     8821 agaatattag attcaaataa gattgattat aaagtaatgt cttatgaagt gaaaagtgaa
     8881 catgtggatg gtgttgaggt ggcacatgat ataggaagag atgtaaatga agtgtataag
     8941 acattagtaa ctcaaggagt tagtaagaat atatatgtat atgtaatacc agtacatgaa
     9001 aacttagact taaaaaaggc tgcaaaagta gctaaagaga aaagtgtaga gatgattcat
     9061 gtaaaagata taaataagct tacaggttat attagaggcg gatgttctcc tataggtatg
     9121 aaaaagtttt ataaaacttt tgtaaatgaa agtgcaaaga atttagatac aattattgtg
     9181 agtgcaggaa aaataggata tcaaatagaa ctatctccat ttgatttaca aagacttata
     9241 aaagtagaat ttgtagatgt aataaaaaaa taagaattac atataaaata tagagcttat
     9301 attctcaata ataggtgttg taatattaat aaaatgaata agaattttaa aataaatatt
     9361 cagttactta tgtgtaattg gtataaaaat attgagaaat tttaaaaaca atggtaaagt
     9421 gtaagtataa tcacgaatat tttttataaa gtaatggagg gatgttcgct tgagaaaaat
     9481 aaaatctgaa caaatcgtcg agcaggtaaa aaaactttgt atagaagcga gcctatattt
     9541 aggagaagat gttttaagtt gtataaaaga aaaagcgaaa agcgaaaaga gtgaagttgg
     9601 aaaaaatata ttgaatatat tagttgaaaa tgctgaaata gctaaagaaa aaaatatacc
     9661 aatatgccaa gatactggta tggctgtatt ttttgttgaa ataggacaag aagttttaat
     9721 tgaaggagac acacttactg atgctataaa tgagggagta agacaagggt atgaagaagg
     9781 ctacttaaga aaatctgttg taagtccaat taacagagta aatacaaaag ataatacacc
     9841 tgctgttatt cattatgata tggtaaaagg tgataaaatt aaaatagaat ttgcagctaa
     9901 gggatttgga agcgaaaata tgagtaagat gaaaatgtta aagccatcag atggattgga
     9961 aggaattaaa aaattcataa tagatacagt ttcagaagct ggacctaacc catgccctcc
    10021 tatggtaatt ggagttggta taggtgggac tgtggacaaa tgtgctcaaa tagctaaaaa
    10081 ggcacttttt agagaactag gtgaatttaa taaggacgaa aatatagcaa aacttgaaag
    10141 tgaactatta acagctatta ataaacttgg tataggacct caaggcttag gtggtactac
    10201 aacagctttg ggattaaaca tagagacttt tcctacacac atagcaggtc tacctgttgt
    10261 tgtaaatata aactgccatg catcaagaca taagaatgta gtaatttagg aggaaaataa
    10321 aatgataaaa ataactacac ctgtaaatga aattgatata gcaaaattaa attgtggaga
    10381 tacaattagt ttgagtggta tactttatac agcaagagat gctgctcata aaagattgat
    10441 agattgtata aataaaggtg aagaattacc atttgacgta tatggtcaag gaatatatta
    10501 tgtaggacct actcctacaa agccaggaga agttataggt gctgcaggtc caacgacgag
    10561 ttatagaatg gatgatttga ctatacctct tttagaaaga ggtcttagat tgatgatagg
    10621 caagggaaaa agaagtgatg aagttataga aggtatgcaa aaatacggag cagtttatct
    10681 tgctgcaata ggaggagctg gagcgtatat ttcaaattca ataaaatcat gtgaaattat
    10741 tgcatatgaa gatttaggag ctgaagctat aagaaagatt gtagtagaag accttaagct
    10801 tacagtagca atagatagct atggaaataa tatatatgag caaggtcgag caatttatga
    10861 atgtaaataa tgtttagaaa taaatatgaa tatcaaaatt aaaaatagtt gtgtatattt
    10921 taaggaggaa aattgatgtc taaggattat gcaaaattgg cattagaaat gcatgaaatc
    10981 aacaaaggga aagtaagtgt agaaagcaag gtagaaatta aaactaagga tgatttatca
    11041 acagcatata caccaggggt tgctgaacct tgtttaaaaa tacatgaaaa tcaagatgat
    11101 gtttatagat acacttctaa agggaatcta gtagcagtag ttagtgatgg aagtgctgta
    11161 cttggattag gtaacattgg tgctgaagca tctatacctg ttatggaagg taaagctata
    11221 ttatttaaac aatttgcaga tgtagacgct tttccaatat gtttaaagac taatgatgta
    11281 gatgagatag ttaaatctgt agagttgatg gagcctgtat ttggaggtat aaatttggaa
    11341 gacatatctt ctccaagatg ttttgagata gaagagagat taaaaaaatc tttaagtata
    11401 cctgtttttc atgatgacca acatgggaca gcaataatag ttgcagccgc cataataaac
    11461 tcaataaagt taattgaaaa taaaaaaata gaagatttag aaatagttat aaacggagca
    11521 ggagctgctg gtatagccat agcaaagata ctacttaata tgaatgttaa aaatataatc
    11581 ttatgtgata gaagtggagc attggaagct agtatagaaa atctaaacta tgttcaaaaa
    11641 gaaatgttaa aagtaacaaa tataagaaat gaaaaaggac ctttaaaaga tattataaaa
    11701 ggaaaagatg tatttattgg tgtttcaggt ccaggagctg ttacaaagga aatggttgaa
    11761 agtatgtctg agaagccaat aatattggca atggcaaatc ctactccaga gataatgcca
    11821 gaagaagcaa aattaggtgg agcatttata atgggaacag gtcgttcaga cttccctaat
    11881 caagtcaata atgtactggc ttttcctgga atatttagag gtgcacttga tgttaaggct
    11941 aaagagataa atgaagaaat gaagatagct gctgcatatg caattgctaa tacaataagt
    12001 gatgaagaaa tatgcccaga gtatatatta ccagatgtat ttaataagaa tgtagtaaaa
    12061 aatgttgcta aggcagttaa agaagcagcg ataaaaacta aagtaaatag aatataaatt
    12121 tttatatgtt catatatcct tatattatcg ggtatatggg catttttatt ataggtttat
    12181 actcatatat ccttactttt atatacatat ctttatgatt aaaggtgtta gaaaatgtag
    12241 taaattttat actatcagac tctatcttta ttactttagt agatacatgg ttgtaaaggt
    12301 ttatagaaaa aaatatagga aggcagattt ttattgttgc gtatatatga gaatgatata
    12361 atatgaatat tataaattaa taaatttaaa tttatggagg tacaagagat gatggaatat
    12421 aaagttaatg accaagaact taatgcttca atgtttatcc cattcgtcaa caggatatgg
    12481 ccaggagatt atgatatgga taaaacacag actgcattat caaaaacttt gaatataact
    12541 gcttatgatg ataaagtgct tgtaggatgt ctgcgagttc tttcagatgg ttattatttt
    12601 ggaacaatta cagaattgct tgttcttcca gagtatcaga aacaaggcat tggtagtaag
    12661 cttttccagc ttgctaaaga gaacacccca actatgctat attttggtgc acagccagga
    12721 gtagaagcgt tctatgaaaa aaatgggtgc caaagaagtc tgcaatccta cataataaaa
    12781 aagaaagata gttattaaag ttccagcttt caataatctt aaaagtacaa tattttagtg
    12841 gaatcatttc attttttatt actaatatga cataaacatg tcatattagt attgatatac
    12901 ttttaatatg gttaagagcc aaagaaaaat ataaggagtg atttaaaatg gaagaaacta
    12961 aattttgcca atgctgtgca atgccaatgg gacaaactga tgaattatat gggacaaata
    13021 aagatggaag taaaagtact gattattgta gttattgttt taaagatggt gagtttacag
    13081 ctgatataag tatggaagag atgatagaag tttgtatccc tcatatgctt caaagtaata
    13141 aggatatgac tgaagatgaa gcaagaacaa tgatgaacaa tttctttcca acattaaaac
    13201 gctggaaata attttatgca aaattagtta tatgcaaaac atgaaatatg gtgttgagaa
    13261 taaggggtgg tgtgctttat agcataccac ctgttttgtt acttggagtt cagttatttt
    13321 cctaaatatt ttaaatatac tattttagat aagttccctc ttcatcttga taaattaatc
    13381 cttttttcat aagcactcca agagctcgtt taaaattttt cttacttgaa ttaaataata
    13441 tagaaatatc tttagggtca gatttatcat taaatctcat atatccatca gaaccttcta
    13501 aataacttaa aatcttattt tctaaagtat caagttcatc ctttctattt cctctaggag
    13561 aaagaccaag ttttccatct tcatatattt ttatgacatg taaattttca agaacatcac
    13621 cagtttttag ttcagtaaag tactcattat gaagtattac accagcatat ttattgtcta
    13681 cacatatcat tgcagaatta ttagtttgga atccatatac aactccagtt acaatatctc
    13741 ctacattgta agtatggtct gttaataagt atgaatctat gtctgtagta gcagcaattc
    13801 ttttgctttt gtctaaatat atataaaata aatatttttc gtctctaaat agtggataag
    13861 tcttagcttt aaaaggaact aatatgtctt taggaagtcc catatctata aaagtaccaa
    13921 tgtctgtatt atcaactact tttaaatgtg ctacatcccc aaccttagct aaaggcggta
    13981 ttaaagttgc agcagttctg tcatcagaat ctttaaatat gaaagcattt acttcatcac
    14041 ctatgtttat ttcatttttt ccgattaatc tattatgaag aagtatatcg tcttttgtgt
    14101 tattagtttg tccatccaaa aaatatccaa attcagtttt tctttttact gtaagtttat
    14161 taaaatcacc tatgtttatc aaattaaaat catcctctca ttttatattt ataatttagc
    14221 cataatatac ttatgtagta gtttgtcaca gtatccatat tagtattagt gaaatttaca
    14281 ataaataata tagttattta tgtatttttc aattctctag cttcttatat agtataaact
    14341 ccaatttctt ttttgtcttt aggttttatt aaaattttta caaaataagt taaaaagtat
    14401 tgaaataaaa tttcttttaa agtataattg gtatatacca aaattggtat ataccatatc
    14461 gaaaagtggt gactatatga aaatagtaaa taaatctagt aatataccat tgcatactca
    14521 attaagttca ataataagag agatgataga aactggtgag cttaaagagg gcgatgcaat
    14581 aatgccagaa agagaacttt gcaacataca aaatgtaagt cgtatgacag taaataaaac
    14641 cattgttgga ttagttacag aaggacttct ttatagggta caaggtaaag ggacattcgt
    14701 tgcaaagcaa aaaaagaagt atcagttttc aaatgtaaaa ggttttactg atgttatgaa
    14761 agaaaaggga gtaaacataa aaacggatat attatccttt gagatggaac taccagatga
    14821 tttagttaaa agaaagcttg gaataagtga taatactaca aatatatata agattgtcag
    14881 acttagatat actgatggag agccatttgg attagaaata gtatatctat cagaagaaat
    14941 gtgtaagggt ctaacaaaag ggatattgga taattcttca ttatataggg ttttaaatga
    15001 aaaatatggc tataagatac aaaaagctga acaggtaatg gagccagtaa tactttcaga
    15061 tgaagaaagt aagcttcttg aaacagatga aggtgcttta gctcttaaac ttcatagaaa
    15121 ttcctataat agagaaggta gccctataga atataccata tcaatattta gaacagataa
    15181 atatcaatat gaaatagtat tgagtgagta ggtgaataat atgaaatgtt taattaatgg
    15241 aaagataata ttaaaaaatc aaatcttaga aaataaagta ttagtatttg atgaaaaaat
    15301 aattgatata gctgatagtg taccaaaaga ttgtgaagtt atagatgctg atggaaaata
    15361 tatatctcca gggcttattg atattcatat acatggaaac atgggaaaag atacaatgga
    15421 ttcgactgat gaatctattg aaacaatttc aaagtcaatt atgagacatg gagtaacatc
    15481 ttttcttcca actacaatga ctatggataa agaacatgtt tatgatgcac ttgaagttat
    15541 taaaaaagcc caaaatagaa agcttgaagg ggcacaagta ttaggagcac atttagaagg
    15601 accttttata aatgagaatt ataagggagc acaaaatgaa aaatttataa taaattcaaa
    15661 atacgaattt ataaaagaat ataaagatgt tataaaagtt ataacttatg ctccagaaaa
    15721 agatattgat tttgatttta ctagagaaat aaaaagatgt acagatatag ttttatctat
    15781 tggtcatagt aatgcaaatt atgaccaagc taaagaagct ataaatttag gagtaactaa
    15841 tgtaactcac atgttcaatg caatgactgg tttaaaccat agagacccag gcgttgttgg
    15901 tgcagcactt acaactaatg tgtacagtga gttaatagca gatacaatcc atataaataa
    15961 agatttattt caatttatac taaataataa aggaaaagaa agactgattt taataacaga
    16021 ttctatagaa gctggtggtc ttgaagatgg caattattct cttggaggtc agaaagtcat
    16081 agttaagggt aatgaagcta gacttgaaaa tggagcatta gctggaagtg tgttgtcttt
    16141 aaataaaatg gtatttaact tcttagataa tacaaacctt aaagtgaatg aagcaattaa
    16201 tttagcttct ttaaatccag caacatcttt gggaataaat gacaaaaaag gaagcttaga
    16261 aataggtaag gatgcagata tagctgtatt tgatgagaat ttagattgta aaatgacact
    16321 ttgcttgggg gacgtagtat ataaaaatat atagtataca aaacaaacct taaagacagt
    16381 aaaaacaata taaaaaataa taaattaaac ttacattagt aaaagtagta gttaaaattt
    16441 aggaggaata agatgagagt attagtgtgt aaagattatg atggaatgag taaaaaagcc
    16501 gctgaaatga tagcagctca aatagtttta aaaccaaatt caatactggg actagctaca
    16561 ggaagtacac cagtaggtat gtatagagat ttagtaaaaa aatacaatga taatatagta
    16621 gatttttcag atgtgatgag ctttaattta gatgaatatt ataagttacc aatatctaat
    16681 gaccaaagct atgattattt tatgaaagaa aatctattta atcatgtaaa cataaagcca
    16741 gaaaatactc atctaccaaa tggaatggct gatgatattg aaaaagagtg tatgaactat
    16801 gaagcttcta tagatgcagc tggtggtata gatgtacaag tacttggaat aggtcgtaat
    16861 gctcatatag gttttaatga acctgatact aaatttgcaa agagaactca tgtagttgaa
    16921 cttacagagt ctacaataga agctaatgca agattcttta aatcaagaga agatgttcct
    16981 aaaaaagctg taagtatggg aataggttca atattgaagt ctaagaaaat attgctttta
    17041 gcttcaggag aagaaaaagc tgatgcagta tataatactg tttatggaga tataactcca
    17101 gaagttcctg gttctatact tcaacttcat aaagacacta tagttatagt agatgaagca
    17161 gcagcaagta aattgaatcc aaaagattat aaattagtat aagaatagaa tgtagtttaa
    17221 gtaaaaaata gctatcattg agtaatcaaa tttatttaat gatagctatt tttatttaaa
    17281 ttttttactt ctttttattt aaattagtta ttattgttta aattacttgc ttattacttt
    17341 ttacgaaaac tcgttatcaa gaagtatcca aataagatgt taaaaattat tatgtttagt
    17401 gggattctat caagcataat aaaattacct ataattggga tattaccaac tggaaacata
    17461 aagaagaata caggcatcag tgatagtaaa aaagatggaa aacaaagaag aagtaatctt
    17521 ggaatatcaa tataccaact accatccttt ttgtattctc taatcaataa ttcagttcct
    17581 agtaataaac caataccacc agaggaaatt aaattaataa aaatgtatct tttaaagtct
    17641 tgcatataac cagtgtaaaa ttgagtatag aaatcataat ttaatttaac agctaaaaaa
    17701 tacataagca atagaatgat taaaaataaa agatatctaa gagctttttt cattttaaat
    17761 tctcccttca gaaatacttt attcagtttt attttgttgg aatctgattt tatcatttag
    17821 tttaagaaat ccataatatt agtgaagaat gaaataagta ttatatatga ataaatattt
    17881 ttagaattaa ggtgaattgt agtgcttaat ataactataa tattaattgt ttgaatattc
    17941 aataaaaagt ttgaaaaata tcaattttga tataaactga ataaatacag ttgaatatta
    18001 aattattatt ttatcaaaaa tatgcaaagt actacacctt caaatatcac ctaaagtaat
    18061 taaaaggcaa ttaaggtgaa gtagttcaag tatttctaaa ataagtttaa taaattctat
    18121 aataatttat tttttgcctt aaaattgcct taaaatccaa acaattttaa aactaatgta
    18181 ctataaaaag ctggtataat ttaaatatag taaaaaataa gatttatctt atctattggg
    18241 atttaattat ttaatcaata tatttatacc atttaattta aataaaaagt ctattaagga
    18301 gtgagcttat gagtaagtta atttatatag cagatgatga agataatata agaaatttgg
    18361 taaagacatt cttaaaaaat gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac
    18421 ttttagaaca atttaatata aaggagtgtg acttagtaat actagatata atgatgcctg
    18481 gctcaagtgg ttttgaagta tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca
    18541 tgcttactgc aagagataca gatatagatt atattacagg gataacctta ggtagtgatg
    18601 attattttac aaaaccattt agcccaatgt ctttggttat gagagtaaaa tctatattta
    18661 gaaggataga gtttgaaaag aaacaaaatt atgataaata tagtaactcg atagatatgg
    18721 aattaaaatt tggtgatgta ataataaata aaaagaataa aatcgttaca agtaaaaatg
    18781 taaatataga ccttacacca aatgaatata acctattgac ttatttgttt gaaaatatag
    18841 atagagcagt ttccagagat gaattattaa acaaaatatg gggatacgat atagaagttg
    18901 agacaagggc tgctgatgat acagtgaaga gattacgtaa aaaaatttta aatacaaata
    18961 ttttaataga aactgtatgg gggtttggat ttaggttgaa ggagaaaagt taagatgaag
    19021 aatggaaatg acaagaatag aattggaaag attaaatcca aatttaaatt aaagttctca
    19081 aagaaaaata taagcataaa aactaggatt ttgttgagta tactttgttt gataattata
    19141 gtatttatga taatattagt atcttttaat gtatttcttg atacatatat aaaaacaact
    19201 gcgaatgaag aattgacaaa gtctacagaa atagtagaac atatggatag taattttaga
    19261 cctattaagc caccacaaga tgatggaaag cttcctccca aagtattatc taattttatg
    19321 agaaatgtac aagataaagt taggatggca gaaacacaat cagatgctga tgctatggta
    19381 gttgattcta agtataacct gatatttcca acaagagagg atgatttctt aaaaaatgtt
    19441 gatgagatgg agctgattag ggagtgtatt caaaatgaaa aattagattt aaattcagat
    19501 gaaaatacac ggatatctac cagaaataag gattattata tttcaactgt aaaaattact
    19561 agtatatatg gtgaacaaga caagtatctt atacttttta tagacatatc aaaaactcta
    19621 aatctagcac aaaagataaa tattgtatta atatctgtaa tgtgttttgc tggaatatta
    19681 gcaatattta cagctgtgat tttatctgag aaaattgcta aaccgataaa agagttgtgt
    19741 gagtttgtaa aaaaaatggg tcaaggagat ttcaaaagaa cctactttga cttttcagat
    19801 aaagaattag tagagctctc aactgttatg aataagagtg ctgagtatct ggataagtat
    19861 gataatgaac aaaaaatatt ttttcaaaat gcatctcatg aattaaggac accacttatg
    19921 tctattaaag gttatgcaga agctataaaa tataatgtta tagatagtaa acatgcaagt
    19981 gatataattc ttgaagaaag tgataggtta agtgatatgg ttgaagattt attatacatt
    20041 tctaaaatag ataatataac taaagatttt gaacttgtag aatgtgattt aagagaagta
    20101 ctttcaaatt gtggagctag gcaaaatgta agagctataa ataaggggat aaaatttatt
    20161 tatgaatttg atgaagaaat ggttttattt gaatgtgatg aaaaaaacat ttccaaagca
    20221 tttatgaatc ttatagaaaa tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt
    20281 aagtataatc aaaaaaatat agttgtgata atagaagatg atggaattgg aatcaagaaa
    20341 gaagatttac ctcatgtttt tgaaagattc tataagggag tagggggaaa tcatggaatt
    20401 ggattatcaa tagttaaatc tattgtaaat aagcatggtg ggcgtatata cgtagaaaat
    20461 ggcaaaaaag gagctaaatt tactatatta tttaaattgt aaaaaaatta gagaaaatat
    20521 agagatgttt gaagttgttt tttatatata tctgttgtat actgttgaaa aaagaacaat
    20581 attaagctga catatattac gatagttgac atttaaagta aatgcttaaa tatagatacg
    20641 aaaggaaaag taaaatctga agtgtattta ggtttaagta agcttcttat tttataaaaa
    20701 atatgtatga aagaacagct gatttttaat attacaaatt tagtaacggt tgtatttgaa
    20761 gcttttgtca tccatatgtt tttatcagac tttttaggtc agaaagaagg gtatataaaa
    20821 gttgtaagat atgctaaact gggttttata atatgtttag gattctgtaa tttaataact
    20881 ttaaacccta aaatcacaat gcctttaatt ttcatcctta ttttttcaac atcattttta
    20941 tataaaggaa atttaaagac acgattgttt actactgttt tattaagtat attctttata
    21001 ctgtcagaaa ttgtagttac aagtattttt gttttatttg taaaagaagg ctttgaaata
    21061 atgcttgaaa ataatagtat aagagtattg gcgacaattt tatctaaaat tgtctttttg
    21121 ttgacttgta aaattatttg tctatttaaa aaggatgtac atttagatat gcccataaag
    21181 tattggctcc ctttattttt aatacctata tttagtttat ttctgtctgt ttctatcttt
    21241 gatgtaagta aattttttag tcttgaaagt ttaaaatttc taagcttaat atctagtgtt
    21301 ggtattttat atattaattt tattgtcttt tatttattta aatttattat agataaaact
    21361 aaactatcta tgaaatatga actacttgaa aaagaaataa ttcataagga agaattaaga
    21421 cttagtaatg agtgttataa aattattgtt gaacagactg attctgtcgt tttcgaatgg
    21481 aatatcaaag aaaataaatc atttgtatcg caggcttgga ctgaaaaatt tggatataac
    21541 aatgcatgta agaatatttt taaagaaatt aaagataaag acttagttca ttctgaagat
    21601 aaagctatat ttgaaggttt tcttgaaagt attaaaaaaa agaatatgca caatcaagca
    21661 gtatatcgtt taaaaaaatc taatggtgaa tatatttggt gtagaacatc aattacaagc
    21721 atttataatg atgaaaatga acttttaaga gttgtcggag taattgtaga tgtagatagt
    21781 gatataaaaa aatatgaaga gttaagaact agagcagaaa gtgactcatt gaccaatata
    21841 tacaataaag gtacatttga aaaattagtg gaagaaacta tagttatgaa tacaggtgat
    21901 aaaaaagatg cattattcat tattgatttg gatgatttta aagaaataaa tgataatttt
    21961 ggtcatccat ttggtgattt tgtactaaaa acatttgctg ataagataca aactagtttt
    22021 ggtagtaaag acttagtcgg aagaattggt ggagatgaat ttgttgtata tatgcaagat
    22081 tatgttacag aagtaaattt gcataaaaaa gctaaagaat taaatagagt actttcggat
    22141 aattacactg atttgagttt tagttttgat gcttctgtca gtataggtat tgcaaggtat
    22201 cctcaagatg gaacttcatt ttttgaattg tttaaaaatg cagatagagc actttatagc
    22261 ataaaagcat caggtaaaaa ttcatattgt ttatttgaag aagaactata tgtacagtga
    22321 atttgaattt agattttatg tgagagtaag atttatatat acttgtggct gtctgattta
    22381 taaaatatgt tactttgtat atttttatat gtttcagtaa ggttaattaa catgagcaaa
    22441 ggtgtaagta aatttatatt agtttaagtt tacttacacc tttataaaaa tacaaataat
    22501 agattcattt acattcttaa atgattaaag ctctttacct tctaaacatg cataacatat
    22561 cgttataatt ccagtaaata agactaaaat gcccattact aagattacaa tttcatcatc
    22621 tattactcca aatgttttta aagtaactta tttcgctttg tatcataata atttgtctct
    22681 ttttacattt taacttctgc tgagatttga gttagatatt cattagaatc tcgcacactt
    22741 atatcatata gaagatattc ttcataaaca aaatccccaa tacgtaaatt attctgatta
    22801 aaaaaattta ataacttttc ataagccttg taaatagttt cataactccc tttatgataa
    22861 gcagttacat aaagtccctt tggtttgatt gatacaggat atttcttgta ctctttatta
    22921 acttttgtaa ataaatatga atatgattca aagtctttat taattatatc ttcaccttta
    22981 atgattgagc caatcgtacc ataatcactc aattcatagt tgtccataaa gttaatacat
    23041 tcatataaaa aattttcttc ctcatctcct aaaaactgtt ctttatatgt aacaggtgtt
    23101 ttgaccaagt actcttcttc atgttctttt aaagtaatag tattcgcatc tatattacat
    23161 gcacttttag taaatgaaat agtagattcc attagagctt gaatattatt tagtttttca
    23221 atttcatttt taatatcaat ggttttcttt tctaaaagct caattagtaa attaggtgtt
    23281 cttttgtcta tataagcttt aatttctttt aaaggcattt ttacttccct aagtgaagaa
    23341 attataccaa aaatgctaag ttgatggtat gagtagtatc tatatccatt ttctttcttt
    23401 aattctggtg agaaaagacc tatatcatca taatggaaaa gtgttttttt attgattcca
    23461 caaatctttg caaattctcc agttgtaaaa taattttttg aaacttgact cataattata
    23521 attcctcctt gactatcctg ttacagtata gtttatcata gtattttgta aaggagtgtg
    23581 attatatgaa aaaattaatt cattttttaa aaccatatag agttttaatt gttgtagttt
    23641 tgatatttac gtttttacaa acacttggaa ctttgtatat accaacactt acagcaaata
    23701 tagtaaataa tggtgtagta aagggagata tagattatat tgtaaaaaca ggtttaatga
    23761 tgatgatagt agctggtata acagctttat cagctgtgtt agtttgtaaa gtatcagcaa
    23821 atctttcatc tggattttgt agagatatta gagaagcagt atttataaaa tcacaagatt
    23881 tatcaataaa tgattttaat aatataggta cagcatcaat gattactagg agtactagtg
    23941 atattacttt aataggtcag tctgtgttta tgtttattca attagtatta ccagcaccaa
    24001 ttataactgt ttctggtcta ttcttagcat attctataga taaagcaatg acaattatta
    24061 ttgttgtagt aatgttttta ttcatgcttt cagcattttt agttggtaaa aagcttataa
    24121 agttgtttaa aatgatgcaa ataaagatgg ataatatgaa tagggtgtta cgtgaggtag
    24181 taactggagt aagagttata agagctttta ataggagtcg ttttgaaaaa aagagatttg
    24241 atagaactgc aatagattat tctgaaacag caatttcaat aaataaaata tttgcagtac
    24301 ttatgcctat tgtaatgtta ataatgaatt taggtatagt aagtattatt tggtttggtg
    24361 ggatgagagt cagtaatgga aatatggaaa taggtcatat aatggcactt gttgaatatt
    24421 gtatcttaat tttattctat ttaatcatgg gagtaatggt atttatgtac atacctagag
    24481 caggtgcatg tgcagataga gtaaatcaga tattagatat agaacctgaa attgtagatg
    24541 gaaatggtca taaagatact gtctcagaga gaggacattt agtatttaaa aatgtaacct
    24601 tttcatatgc acaatcagaa gaacctgtgt taaataatat aacatttgaa gcaaaatctg
    24661 gagaagtaac tgcaattata ggaagcactg gctcaggcaa atctactatt gcaaatataa
    24721 taccacgttt ttttgaaatt caatctggag aaatatctat aaatggtcag gatattaaga
    24781 agattcctca aaaagaactt agagataaga ttggatttgt accacaaaaa gcttttttat
    24841 ttagtggaac aatagaagaa aatataaggt atggaaaaga agatgcaagt attgaagaag
    24901 taaagcatgc tgcaagtata gctcaagctg atgaatttat ttatgatatg gaagacaagt
    24961 atgactcatt tgtagcacaa ggtggaaata atttgtcagg tggtcaaaaa cagagaatat
    25021 ccattgcaag agcgttagtt agaaagcctg aagtatatgt ctttgatgat agtttttctg
    25081 cattagattt taaaacagat aaaagactta gaaaagcgtt gaaaaatgaa atcaaagatt
    25141 catcagctat aattatagca caacgtataa gtacaatcat ggatgctaat cagattatag
    25201 ttttaaatga tggaaaaatt gtaggtattg gaaaacataa agatttactt gaaaattgtg
    25261 aagtttataa gcaaattgcg gattcacaac ttagtaaaga agaacttgca taaaatgaag
    25321 aattaatctg atatttggag gtgtatgata tgagtgaaaa aagaatagat gaacaagata
    25381 tagaagtact tagttttgaa gatggcatgt cagcagaaaa agctaaggat gcaaaaaaaa
    25441 ctactaagag attattaaaa tatatggcaa aacaaaagtt taaaatatta attattttta
    25501 tttcagtatt gataagttca gcgcttacag ttttagcacc tatggttatg ggaaaagcta
    25561 ttgaccaact atttaatgga ataaaaactg cagtccaaac aggtacaaaa ttcagtgtga
    25621 attttagtac tatgggaggg atagtatcaa tacttctagg gttgtactta attagtgcag
    25681 tatttatata tattcaacag tatattatgt caggagttgc acaaaatcta gttcttagca
    25741 tgagaaaaga tttaagtgat aaactcaata aacttcctct taagtactat gattctcata
    25801 aaaaggggga aacacttagt atagtaacaa atgatttaga aaaagtagca gatagtttac
    25861 aagaaggatt aatgcaactt attacagcag tagttacagt agttggttct attgtaatga
    25921 tgataagtat aagtgtacca cttacaatag tatcagctat aacattactt gtttcattgg
    25981 gtattactgt tgtaattgca agaaaatcac aagaaagatt ctctgaaaat caaaaggcac
    26041 taggagagtt aaatagcaat atagaagaga tatttactgg tcaaatagtg gttaaagcat
    26101 ttagtaaaga aaaggatact ataagaaact ttaaaatatt aaatcaaaat ctttataatg
    26161 caagtagaaa agcacagttt tcgtcatatg caatatctcc aattattaga tttataaacc
    26221 taattggcta tgttattata gcagttgtag gtgggatatt tgcatcaact ggagcaatga
    26281 ctcttggtag tatacaagcg tttattcaat atgtaaatca agcatcagag cctactacag
    26341 aaatatctta tattgtaaat atgcttcaag cagcaatagc atctgcagag agagttttta
    26401 cagttatgga cgaggtggaa gagataaagg atgaagaatc ttcaaaggtt attagcatgc
    26461 ctaaaggtaa agtacaattt gagcatgtta aatttggata tagtgatgat tttattctta
    26521 tgaaagatat taatatcaat ctaaatgcag gagataagat tgcaattgtt ggacctacag
    26581 gagctggaaa gacaactctt gtaaatttac ttatgagatt ttatgagata caaggtggaa
    26641 gaattactat tgatggtgtt aatataaaag atttaaagcg tggagaactt agaactatgt
    26701 ttggtatggt tcttcaagat acatggctgt ttaatggaag tataaaagaa aatattgcat
    26761 atagtaagtc agatgccaca atggatgaaa ttgtaagagc agcaaaatca gcaagagtag
    26821 accattttat aaggacttta ccacaagggt atgacactat attgactgag gatgcttcta
    26881 atctatctca aggtcaaaag caacttctaa caattgccag ggcaatactt tcagacccat
    26941 cagtattgat tcttgatgaa gcaacatcta gtgtagatac tagaacagag gtagaaattc
    27001 aaaaggcaat gaataacttg atgaagggaa gaactagttt tgttattgca catagattat
    27061 caactattcg agatgctgat ttaatattag taatgaaaga aggaactatt attgaacaag
    27121 gaagccataa ggaacttatt gaaaagaaag gattctatga agaactttat aacagtcaat
    27181 ttacaagtga atatgatgaa gatgttgtat gattatcaag ttttaggcaa ctaaatgatt
    27241 gataaatatt aagttaaata tagtaacatg taaggttata tggaataaga gtatttctct
    27301 atttcatata acctttttta tttatcaaaa taagataaaa tttcctttct aaattagatt
    27361 gaagaatgta tttatcaata taaaagtaaa ataatcatta ttattagtga agtaaatatt
    27421 gtttgccaca tatatgccat aaagatgcac ttattttacg gagatttaac aaagtagtgt
    27481 aaagtatact ctaaatataa aataaatcaa atagagttaa ataaattgag aggtgatata
    27541 aatagtgaat aaaaaattga actatagatt tgaaatgaag cacaaaataa ctgaagcaga
    27601 tgtgttagct ttaaaatcaa gactctatcc aataatgaag aaagatgaaa atgcatctaa
    27661 agatggaaaa tatcttatta ggagtttata ttttgatact cctgaagata aagcactttt
    27721 ggataaatta aatggcgttg caataagaga aaagtttagg ataagatttt ataataatga
    27781 ctgctcttat ataaggttag agaaaaaaat caaacattac aacatgacat caaagctatc
    27841 aactaattta accaaaaaag aagtaataaa tatactaaat aatgatattg aatttctaaa
    27901 agagtcgaca aattgtttat taagagaatt ttatttgaaa ttaaaatgtg aaagattaga
    27961 ggcaaaaagc atagttgatt acaatagaga agcttttatt tatccagttg gaaatgtaag
    28021 agttacaatc gatagtgata ttaaaacttc tgtaaattcg gtagatttat ttaataaaga
    28081 tttgccaact atttcggtaa ttgatgagaa tatgactgta ttggaggtta aatatgatga
    28141 atttatacca gattttataa aggatttaat tcaaattaat aagactacct ctacagcagt
    28201 ttcaaagtat gcttcaagta gattatatat ttaagaataa aggctatgat ttatataaag
    28261 atatacataa ataaggttaa aaaacaaata agttaaaaat aaataaaata aaaaataaat
    28321 aagttaaaaa taggaaggat gattttaatt atgacattta atgatatttt taaatcaagt
    28381 tttatagaaa acgtatcagg tttttcattt gtggacagtg ctttagcact aggttcagct
    28441 tttttagtag gtttgtttat atatatggtc tataaaaaga cttatatggg aattatgtat
    28501 tctcgaccat tcaatgtgtc gcttgtagca cttacaatgc ttacaacttt tgtaatattg
    28561 gcggttactt caaatgtagt tttatcctta ggtatggttg gtgctttatc tatagtaaga
    28621 tttagaacag caattaaaga cccaatggat ttagtatttc tattttggtc attaggttca
    28681 ggtatagttt taggagcagg tttgattccc ttagctgtca tgggttcaat aattatggga
    28741 ttgattctaa tattcttttc aaataaaaca atatctgaaa ctccatatat attgatggta
    28801 aattgtaata atgaagattc agaagatata gcaactgata aaattaaaaa agtatttact
    28861 aaatatcaaa taaaatcaaa aagtgttaca ccagaaaaag gaatagaatt agtgtttgaa
    28921 gtaagaatga aagatggaga aacaagtctt ataaatgact tgagtcaagt agatggagtt
    28981 actaatgcag ttcttgtaag ttataatggt gactatgtag catagagagg agtaatcttt
    29041 tatgaaagat aaaaaattta cccttcttat ctcgattatg attatatttt tatgtgctgt
    29101 agttggagtt tatagtacat ctagcaacaa aagtgctgat ttatatagtg atgtatatat
    29161 tgaaaaatat tttaacagag acaaggttat ggaagttaat atagagatag atgaaagtga
    29221 cttgaaggat atgaatgaaa atgctataaa agaagaattt aaggttgcaa aagtaactgt
    29281 agatggagat acatatggaa acgtaggtat aagaactaaa ggaaattcaa gtcttatatc
    29341 tgtagcaaat agtgatagtg atagatacag ctataagatt aattttgata agtataatac
    29401 tagtcaaagt atggaagggc ttactcaatt aaatcttaat aactgttact ctgacccatc
    29461 ttatatgaga gagtttttaa catatagtat ttgcgaggaa atgggattag cgactccaga
    29521 atttgcatat gctaaagtct ctataaatgg cgaatatcat ggtttgtatt tggcagtaga
    29581 aggattaaaa gagtcttatc ttgaaaataa ttttggtaat gtaactggag acttatataa
    29641 gtcagatgaa ggaagctcgt tgcaatataa aggagatgac ccagaaagtt actcaaactt
    29701 aatcgttgaa agtgataaaa agacagctga ttggtctaaa atcacaaaac tattaaaatc
    29761 tttggataca ggtgaagata ttgaaaaata tcttgatgta gattctgtcc ttaaaaatat
    29821 agcaataaat acagctttat taaaccttga tagctatcaa gggagttttg cccataacta
    29881 ttatttatat gagcaagatg gagtattttc tatgttacca tgggatttta atatgtcatt
    29941 tggtggattt agtggttttg gtggaggtag tcaatctata gcaattgatg aacctacgac
    30001 aggtaattta gaagacagac ctctcatatc ctcgttatta aaaaatgaga catacaaaac
    30061 aaaataccat aaatatctgg aagagatagt aacaaaatac ctagattcag actatttaga
    30121 gaatatgaca acaaaattgc atgacatgat agcatcatat gtaaaagaag acccaacagc
    30181 attttatact tatgaagaat ttgaaaaaaa tataacatct tcaattgaag attctagtga
    30241 taataaggga tttggtaata aagggtttga caacaataac tctaataaca gtgattctaa
    30301 taataattct aatagtgaaa ataagcgctc tggaaatcaa agtgatgaaa aagaagttaa
    30361 tgctgaatta acatcaagcg tagtcaaagc taatacagat aatgaaacta aaaataaaac
    30421 tacaaatgat agtgaaagta agaataatac agataaagat aaaagtggaa atgataataa
    30481 tcaaaagcta gaaggtccta tgggtaaagg aggtaagtca ataccagggg ttttggaagt
    30541 tgcagaagat atgagtaaaa ctataaaatc tcaattaagt ggagaaactt cttcgacaaa
    30601 gcaaaactct ggtgatgaaa gttcaagtgg aattaaaggt agtgaaaagt ttgatgagga
    30661 tatgagtggt atgccagaac cacctgaggg aatggatggt aaaatgccac caggaatggg
    30721 taatatggat aagggagata tgaatggtaa aaatggcaat atgaatatgg atagaaatca
    30781 agataatcca agagaagctg gaggttttgg caatagagga ggaggctctg tgagtaaaac
    30841 aacaacatac ttcaaattaa ttttaggtgg agcttcaatg ataataatgt cgattatgtt
    30901 agttggtgta tcaagggtaa agagaagaag atttataaag tcaaaataag attaaaattg
    30961 aatataaata attttaatac tgttaagtct aaatatttac ttagcagtat ttttttagaa
    31021 aataatgtat ttggttaata atataaggta ataaattgat aaaaattaaa tacagcatat
    31081 taataataca taataaagta ataaaaaaac tgtttaagta taataactta tatttagaca
    31141 gtttttttca tttataaata atataaatga aattgaaaac atttgaagga tagaaattgg
    31201 ttggataaat gtataaatta cttcaccaaa aaataatgac aaaaattgta actaagttgt
    31261 aataaaatgg tagttattta gcataaaata atgtatcata gaagtatatt ataaaattaa
    31321 taaaataaga tttttgtaag aaaactgctg gcttttgata tttagagtga gtagttaaat
    31381 tatgtttagg aggggtagaa tttgttgcaa aaagtattat ggaaattttt aaagctagta
    31441 gtcggtttat ttatatgttc agttggtata gtattgacga taaattgtaa ccttggatta
    31501 tctccctggg atgtatttca tcaaggactt tcaaatcata taggaataac tataggaact
    31561 gcttctataa ttgtaggctc tatagtagtt attgctgatg tcgttcttgg agaaaatgta
    31621 ggatggggaa ctgtttttaa catgctacta attggattct ttatggattt acttttatat
    31681 agtaatttaa ttccagaggc tgatagttta tttgtaggaa taattatgtt gatattagga
    31741 ttggttcttt tgtctgttgg aatggtattt tatatgggaa gtggtttagg aagtggacct
    31801 agagatggtt tgatggttgc aattcaaaag aaaacaggaa aatcacttaa attaataaga
    31861 ggtactatag aagttggagc attaatagta ggatttttat taggtggaaa agttggtata
    31921 ggaactataa taagtgcatt tggattgggg tatttcacac aaatggtatt tggcttattt
    31981 aaattagatt gtagtaagat taaacataga tttatagtag atgatataaa atttatcaaa
    32041 gtgtatatga gtggtgagaa aaaagctgct gttcaaaata tagttaagta atttaagaat
    32101 aaattcaacg aaatgttaag tattttaaat taacattttg tttagataga taaattgaat
    32161 attaaaagaa tattaaaata tgaggattgc tagaacacta taaaatatag atttctagca
    32221 attttttatt tgttcaatat ttttttagta ctagaagtct tattctctat ttaatgaatg
    32281 aattaattta taattagaga tactaaactt tatagatatt aatataaaca cctttaaact
    32341 gtgtattttc caaagccttt aaaatgcttg tatttattct aaataaaaaa ataaaaaaat
    32401 gtcaaaaaaa ctcttgtatt atatccaaat tttatatata attacctatg ttgtttagaa
    32461 attttaaact caatgtttag taaacaaaat gtttagtaaa ataaaacaga aactaggagg
    32521 tgcaacatgg atataggtga aaagataaaa agattaagaa cagaaaaaca actgacccag
    32581 gaagagttag ctaatagatg tgaattgtca aagggattta tttctcaatt agaaaataac
    32641 ttaacatcac catccatagc tacccttata gatatacttg aaatactagg tacaaattta
    32701 agggaatttt ttaatgaaat agatgatgaa agaatcagtt ttacaaaaga agatatgttt
    32761 gaaacagaag atgaagattt aaaatataaa ttaaagtggt taattccaaa ttcacaaaaa
    32821 aatgaaatgg agccaataat aataacattg tatccaggag gacagtataa agaagaaaag
    32881 ccacatgaag gtgaagaatt tggatatgta ttagcaggtt caatatatgt ccatataggc
    32941 gaaaagaaaa ataaagtaaa aaaaggagag agtttttact ttagaccgaa agcaaatcac
    33001 tatatatcaa acgagggcaa aactacagca aaagtgattt gggtaagtac gccaccatca
    33061 ttttagaata gaggtgttta ataattggta gaaaatataa tagaattaag ggatatatct
    33121 aaacattatg aagaacttac tgttttagat aatttcaatt tagatataaa gaaaaatgag
    33181 tttctaacat tacttggacc aagtggatgt ggaaaaacta caacattaaa aataatagca
    33241 ggatttgagt atgctgatga tggtaaagtt ttatttgaag gtaaggagat aaataatttg
    33301 ccaccatatg aaagacaagt aaacacagta tttcaaaaat atgctttatt tccacacatg
    33361 gatgtttatg aaaatgtagc ttttggatta aaaattaaaa agactccaaa agatgtaata
    33421 gatgctaaag taaaagaggt tttgaaactt gttgcactag aaggttttga aagacgacaa
    33481 gttgaatcat tgagtggagg acaacaacaa agaattgcaa tagcaagagc tctagttaat
    33541 gaaccaaagg tattattact agatgaacca cttggagcac ttgatttaaa gttaagacaa
    33601 gagatgcaaa tagaattaaa gagaatccaa aaaaaattag gaataacatt tgtttttgta
    33661 actcatgacc aagaagaggc tcttactatg tcagatacta ttgttgtaat gtataagggc
    33721 aaaattcaac aaatgggaac accacaagat atatacaatg aacctaaaaa ttcatttgta
    33781 gctaagttta taggtgaaag taacatattt gatggaatta tgatagaaga ttataaagtt
    33841 aatttctgta atagagattt tgagtgtgtt gataaaggat tcgaaaaaaa cgaaaatata
    33901 gaagtagtaa taagaccaga agatataaaa atggtaaagc cagaagaagg aatgttaaag
    33961 gggaaagtta catctacagt gtttaaagga gtgcattacg agatagaatt gaatgaaaat
    34021 ggaagattgt ggatacttca taatactaaa aatgctgagg ttgggactga actaggcatg
    34081 gatatatatc cagaagatat ccatataatg agaaaagaga ataactaaga tgaaaagaaa
    34141 atctttttta gcatatccat atgtagtttg gagtgcgata tttgtaataa ttcctttaat
    34201 attagtagta ttttttagtt ttacaaaaga atcaggtgga ggttatgcct ttacacttga
    34261 gaattataaa gaagtaattg accctatata tatgaaagtt tttgggcgtt ctattctttt
    34321 agcaggagga gctacattaa tatgccttat agttggttat ccagtggcat atataatttc
    34381 aaaggctcgt gtaagtagaa gaggttcatt aatattattg tttattcttc ctatgtggat
    34441 gaactttctt ttaagaacat atgcatgggt tgctatatta ggaaaaaatg gacttttaaa
    34501 tacattttta ggttggtttg gaatacaacc attagcaatt ttgtatacta attttgccat
    34561 acttttaggt atggtgtata actttttacc atttatggtt cttccaatat atacagcact
    34621 atcaaagatg gataatgatt tgataaacgc tgctcatgac ttgggtgcaa ataatatgac
    34681 agtttttaga aaaattatat ttcctttgag tttgccaggt gtaatgtctg gaataactat
    34741 ggtatttatg ccagcagtca ctacttttgc aatatcaaga ctgttaggtg gaggtaaaat
    34801 aatgctggtt ggagatttga ttgaacaaca gtttacagtt gttggagatt ggaactttgg
    34861 ttcagctata tcgatattta tgatgatagt aatattaata tctatgtcta taatgtcaaa
    34921 atttggagat gaatctgaca aagaaggcgg tgggttacta ttttaaaatt aaagaaaata
    34981 acttctaata tatatttagc attagtattc ttatttttgt atgcacccat atttgctttg
    35041 gtactatttt ctttcaatga ttcaaaatca atggcaagat ggaatggatt tacatggaag
    35101 tggtatggtc aattacttca aaatgagagt ataatgagtg ctttatatta tactattgta
    35161 atagctatat tagcgtctgt aatatctaca atagtaggaa ctataagtgc tataggaata
    35221 cataagatga gaggtaagag taagaaatta atactaaatg taaactatct tcctatttta
    35281 aatacagaga tagttacagc agtagctctt atgagtctgt ttgtatttgt aaaaatggaa
    35341 tttggattta caacaatgct attggcacat ataatgtttt gtttgccata cgttatactt
    35401 tcagttttgc caaaaatgaa gcaattacct gataatatag aagatgcagc tatggacttg
    35461 ggagcaacac ctatatacgc tcttagaaaa gtaattttac cacaaataaa accaggtata
    35521 gtttctggat ttttaattgc atttactatg tcaatagatg actttataat aagtttcttt
    35581 aatgctggaa atggtgtaag caatctttct atagaaatct atggaatggc tagaagaggt
    35641 ataaaacctg aaataaatgc attatcaaca ataatgtttg cagtggttct aggtttactt
    35701 ttactagcta ataaaaaaga atctatagta agggggatta aataatgatt aagtttaaaa
    35761 aagcaatatc ccttatagct gtaggtatga tgacagttac tttatttaca ggatgtaata
    35821 aagcaactga ttctacaaaa gttttaaatg tatataatgt tggagattat atagatgagt
    35881 ctcttataga taagtttgaa aaagaaacag gaatagatgt tcaatattca acttatgata
    35941 ctaatgaaat gatgtatcaa aaagtaaaga gtggtagtac acactatgat ttagtatttc
    36001 cttcagacta tatggtagag aaaatgaaaa atgaaggtct tttggaaaaa ttagatttta
    36061 agaatatacc taatatgaaa tatttagata agagtttttt aaatcctgta tacgacaaaa
    36121 ctaatgaata tagtgttgca tatatgtggg gaactcttgg aatactgtac aataagaaag
    36181 aagttaaaga ccctatggat agctggaata tactttggaa tccaaagtat aagggaaata
    36241 taatgatgtt tgactctgtt agagatacta tagggataac tcttaaaaaa ttaggatata
    36301 gcatgaatag tgtaaatcca aaagaaataa atgaagctaa aaatctttta atgaaacaaa
    36361 aggatttagt attggcttat gtaaatgatg aaggtaagga tagattgctt ggtggagaag
    36421 tggcaatggg tatgctgtat tctggagatg cagttacact aatggagcaa aatcctgacc
    36481 ttgattatgc tatacctaaa gagggaacta ataaatgggt agatgctatg tgtgtaccta
    36541 caacagcaca aaataagaaa gaagctgagc tgtttataaa ctttttatta gacccagaga
    36601 atgcaaaagt aaatgctgag tacattgggt attcaacacc taatacagga gcactaaaat
    36661 tattagaccc agaaataact cagaatcctg ttgcatatcc atctaaagaa gtgcttgata
    36721 aatgtgaaac ttttgaagat attggtgaaa atataaaatt atatgacaag gcttggatag
    36781 agttaaaatc aaaatagggt cttgttggtt taagtaaatt tacttaataa atacttaata
    36841 aaactgttct tttttagaaa atttattttc taattattga acagttttat tttttgttta
    36901 agttatattc atatgcaatt taaatagttt attaataaat gaaaacttat ttgtataatg
    36961 actttaataa taattagaaa aaatgggcaa cttaatagaa taattggagg tgtaaattta
    37021 taaaaaatga ataggataaa taaaaaaata gatttattaa tattggcttt gctcgttttt
    37081 atatttttaa ttgttggaat agtaatacta tcgattagga cagaaaataa attgaatgtg
    37141 ttcataatgt tagcaataat tttttttata ataatgttaa cttatttatc aaattcagta
    37201 gtggggttga taacaagttc aataattata tttatgtata catcctatat tttatataac
    37261 aatattactc acaatatgga tgttgaattt atatcatata tgtggataat agcaacacca
    37321 gtatcttcaa ttataatggg taatctgaac aaaagcataa atgaattgca aaatacaaat
    37381 aaaaaactat ctgaacagta taaggaatta gtgactattg atagtgaaac tggacttaga
    37441 aatttaaaga ttttttataa tgatgtaaat atggagataa gtaaatctat tagacataat
    37501 actgattttt ctcttatgat agtaaaatta ccatactacg gaaacctaca gactatattt
    37561 ggtgaaaaca aaactaataa aatagtaaaa tatattggtt caaatattat agaatgtaca
    37621 agaaatgaag atataattta tagtttacaa aaagatatga tagggatact aatgccaaat
    37681 acatctttgg aaggttcaaa agttgttaaa gacagaataa agaaaaggat aaaagaatta
    37741 aatttagatt taaacaatag gggaaaatat gtaaatatag atgtcaaaat tgcattttta
    37801 caatataaga gtagttttgg agatagtata aattttaaga atatagttga agaggaactt
    37861 caatatgatg tttaaaatat ttaataaaag tctattaaaa ttagtagttg tattatcaat
    37921 agtactgaca atatcaaatt ttagcttatc ttcatatgca tcagaaatta cttccagcac
    37981 agataaggta ttaatcatat atgattctaa gaaagaaaca gcttataata gagatatttt
    38041 aaatattatg aggactctat tgggaagatt tagttcagat atagagctat taaaattgag
    38101 taactatgat ggtgagataa ataaaaatta ttacagtcat atatttattt taggaatcaa
    38161 tgaaaactca tataataatg ataaaaacac taaaaacttg attagttcct taaatttata
    38221 taaaggaact atatgttggt taggatatgg tatagaaaat cttttggaac ataaaaaata
    38281 caatttagac tatgtaggaa aaacaaacaa tatagttagt gtaaattata gaggtaaatc
    38341 atataacttg gatgaacatt atgtttttaa cattgtagaa tcaaaagata caagtaataa
    38401 agtaatagga agtattaatg ataccttaaa taagtatcca tatataataa atgataaaaa
    38461 tcttttttat gtatcaaaat tagatttaga tggagtttta ttttatatat tttgcgattc
    38521 acttaatgat atttttaata ttaagacgtt tgataaagga cgtatatttg taagaataga
    38581 agatgtacat gcttttagag aaccaaaaaa tcttgtagag atagcggatt atttaagtag
    38641 taagaatata ccatttacga ttgcattaat accagcatat gtaaatccta aaaatcataa
    38701 ggtaataact ttatcagaaa gccctgaaat tgtcaaagca ataaaatata tgcaagataa
    38761 aggtggaact gtgatattgc atggatatac tcatcaatac aagaaagaag aagtttctgg
    38821 agaagggtat gaattttggg atggtaaaaa agatgaaccc ttaaaagaaa atatgaagat
    38881 atttgtcaaa gatagagttt tgaatggtct tagagtatgt atagagaatg gaatttatcc
    38941 tcttgcattt gaagcgccac attatgcaat ggaatcagag ggatataaag aattaaaaaa
    39001 atatttttct acttatatag ggcaacatca aaataatgat aaaaaattta gcacaaatac
    39061 atatccttat attattaggg atacagagga gtttaatata tttatacccg aaaatttagg
    39121 atatatagac ccagaagata agtttacttt tcaaaatata aaagaaaatt tagataaatt
    39181 atccattgta agaggtttta gtggagggtt cttctttcat tcatatttaa acatagagta
    39241 tctaaaaaac acaatagaat atttagaaaa acagaatata gaatttatga atttaagaga
    39301 ttttaataat tgggtaaaag ttgatgaaat acaaataaga aataatggag atgaaattat
    39361 agtcaactat gataaggact tagatgaact aactaaaagt gatactagat ttaaatccat
    39421 atccaatatt agtaaagttt taatattcat agtatctata agtgtt
//
LOCUS       DAYAOY010000031        38596 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYAOY010000031 DAYAOY010000000
VERSION     DAYAOY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.031"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="ACGI6B_001664"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="ACGI6B_001664"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="ACGI6B_001665"
     CDS             275..466
                     /locus_tag="ACGI6B_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFG9751111.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="ACGI6B_001666"
     CDS             621..2030
                     /locus_tag="ACGI6B_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9751112.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="ACGI6B_001667"
     CDS             2161..2850
                     /locus_tag="ACGI6B_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFG9751113.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="ACGI6B_001668"
     CDS             2853..3788
                     /locus_tag="ACGI6B_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9751114.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="ACGI6B_001669"
     CDS             3785..4678
                     /locus_tag="ACGI6B_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFG9751115.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="ACGI6B_001670"
     CDS             4756..5502
                     /locus_tag="ACGI6B_001670"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFG9751116.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="ACGI6B_001671"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="ACGI6B_001671"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFG9751117.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="ACGI6B_001672"
     CDS             6359..7063
                     /locus_tag="ACGI6B_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFG9751118.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="ACGI6B_001673"
     CDS             complement(7251..7679)
                     /locus_tag="ACGI6B_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFG9751119.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="ACGI6B_001674"
     CDS             complement(7716..9146)
                     /locus_tag="ACGI6B_001674"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFG9751120.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="ACGI6B_001675"
     CDS             9278..9862
                     /locus_tag="ACGI6B_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HFG9751121.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="ACGI6B_001676"
     CDS             9878..10399
                     /locus_tag="ACGI6B_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HFG9751122.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="ACGI6B_001677"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="ACGI6B_001677"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFG9751123.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="ACGI6B_001678"
     CDS             12762..13532
                     /locus_tag="ACGI6B_001678"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFG9751124.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="ACGI6B_001679"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="ACGI6B_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFG9751125.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="ACGI6B_001680"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="ACGI6B_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFG9751126.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="ACGI6B_001681"
     CDS             14894..16009
                     /locus_tag="ACGI6B_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HFG9751127.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="ACGI6B_001682"
     CDS             15999..16868
                     /locus_tag="ACGI6B_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFG9751128.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="ACGI6B_001683"
     CDS             16861..17727
                     /locus_tag="ACGI6B_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751129.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="ACGI6B_001684"
     CDS             17768..18481
                     /locus_tag="ACGI6B_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFG9751130.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="ACGI6B_001685"
     CDS             18482..19651
                     /locus_tag="ACGI6B_001685"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFG9751131.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="ACGI6B_001686"
     CDS             19655..20491
                     /locus_tag="ACGI6B_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9751132.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="ACGI6B_001687"
     CDS             20492..21487
                     /locus_tag="ACGI6B_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751133.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="ACGI6B_001688"
     CDS             21505..23193
                     /locus_tag="ACGI6B_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFG9751134.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="ACGI6B_001689"
     CDS             23205..24272
                     /locus_tag="ACGI6B_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751135.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24756..24872
                     /locus_tag="ACGI6B_001690"
     CDS             24756..24872
                     /locus_tag="ACGI6B_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HFG9751136.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            25036..26103
                     /locus_tag="ACGI6B_001691"
     CDS             25036..26103
                     /locus_tag="ACGI6B_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751137.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26295..26825
                     /gene="rnmV"
                     /locus_tag="ACGI6B_001692"
     CDS             26295..26825
                     /gene="rnmV"
                     /locus_tag="ACGI6B_001692"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFG9751138.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26996..27865
                     /gene="rsmA"
                     /locus_tag="ACGI6B_001693"
     CDS             26996..27865
                     /gene="rsmA"
                     /locus_tag="ACGI6B_001693"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFG9751139.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27931..29193
                     /locus_tag="ACGI6B_001694"
     CDS             27931..29193
                     /locus_tag="ACGI6B_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751140.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29344..30459
                     /locus_tag="ACGI6B_001695"
     CDS             29344..30459
                     /locus_tag="ACGI6B_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9751141.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30645..31541
                     /locus_tag="ACGI6B_001696"
     CDS             30645..31541
                     /locus_tag="ACGI6B_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9751142.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31611..32408
                     /locus_tag="ACGI6B_001697"
     CDS             31611..32408
                     /locus_tag="ACGI6B_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751143.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32463..33815)
                     /gene="murC"
                     /locus_tag="ACGI6B_001698"
     CDS             complement(32463..33815)
                     /gene="murC"
                     /locus_tag="ACGI6B_001698"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFG9751144.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34034..34912
                     /gene="purR"
                     /locus_tag="ACGI6B_001699"
     CDS             34034..34912
                     /gene="purR"
                     /locus_tag="ACGI6B_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFG9751145.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35068..35346
                     /gene="spoVG"
                     /locus_tag="ACGI6B_001700"
     CDS             35068..35346
                     /gene="spoVG"
                     /locus_tag="ACGI6B_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HFG9751146.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35559..36938
                     /gene="glmU"
                     /locus_tag="ACGI6B_001701"
     CDS             35559..36938
                     /gene="glmU"
                     /locus_tag="ACGI6B_001701"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFG9751147.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37033..37983
                     /locus_tag="ACGI6B_001702"
     CDS             37033..37983
                     /locus_tag="ACGI6B_001702"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFG9751148.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT        15473 a         4231 c         6960 g        11932 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaagaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24601 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24661 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24721 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24781 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24841 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24901 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24961 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    25021 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    25081 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    25141 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    25201 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    25261 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    25321 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    25381 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    25441 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    25501 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    25561 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25621 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25681 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25741 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25801 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25861 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25921 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25981 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    26041 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    26101 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    26161 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    26221 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    26281 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    26341 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    26401 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    26461 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    26521 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    26581 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26641 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26701 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26761 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26821 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26881 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26941 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    27001 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    27061 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    27121 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    27181 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    27241 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    27301 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    27361 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    27421 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    27481 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    27541 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27601 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27661 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27721 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27781 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27841 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27901 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27961 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    28021 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    28081 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    28141 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    28201 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    28261 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    28321 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    28381 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    28441 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    28501 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    28561 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28621 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28681 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28741 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28801 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28861 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28921 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28981 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    29041 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    29101 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    29161 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    29221 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    29281 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    29341 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    29401 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    29461 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    29521 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    29581 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29641 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29701 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29761 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29821 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29881 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29941 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    30001 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    30061 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    30121 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    30181 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    30241 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    30301 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    30361 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    30421 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    30481 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    30541 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30601 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30661 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30721 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30781 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30841 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30901 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30961 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    31021 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    31081 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    31141 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    31201 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    31261 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    31321 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    31381 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    31441 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    31501 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    31561 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31621 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31681 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31741 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31801 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31861 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31921 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31981 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    32041 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    32101 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    32161 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    32221 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    32281 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    32341 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    32401 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    32461 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    32521 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    32581 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32641 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32701 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32761 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32821 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32881 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32941 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    33001 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    33061 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    33121 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    33181 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    33241 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    33301 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    33361 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    33421 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    33481 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    33541 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33601 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33661 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33721 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33781 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33841 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33901 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33961 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    34021 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    34081 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    34141 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    34201 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    34261 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    34321 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    34381 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    34441 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    34501 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    34561 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34621 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34681 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34741 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34801 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34861 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34921 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34981 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    35041 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    35101 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    35161 catgatataa aagttataga aggccataat gggctattta tagcaatgcc tagcagaaaa
    35221 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    35281 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    35341 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    35401 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    35461 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    35521 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    35581 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35641 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35701 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35761 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35821 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35881 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35941 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    36001 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    36061 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    36121 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    36181 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    36241 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    36301 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    36361 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    36421 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    36481 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    36541 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36601 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36661 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36721 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36781 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36841 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36901 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36961 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    37021 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    37081 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    37141 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    37201 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    37261 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    37321 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    37381 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    37441 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    37501 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    37561 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37621 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37681 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37741 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37801 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37861 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37921 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37981 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    38041 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    38101 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    38161 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    38221 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    38281 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    38341 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    38401 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    38461 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    38521 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta
    38581 gcaaaggatg gaaatg
//
LOCUS       DAYAOY010000032        38144 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYAOY010000032 DAYAOY010000000
VERSION     DAYAOY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.032"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            251..889
                     /locus_tag="ACGI6B_001703"
     CDS             251..889
                     /locus_tag="ACGI6B_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751149.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1362..2720)
                     /gene="rlmD"
                     /locus_tag="ACGI6B_001704"
     CDS             complement(1362..2720)
                     /gene="rlmD"
                     /locus_tag="ACGI6B_001704"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFG9751150.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2895..4655)
                     /gene="pyk"
                     /locus_tag="ACGI6B_001705"
     CDS             complement(2895..4655)
                     /gene="pyk"
                     /locus_tag="ACGI6B_001705"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFG9751151.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4707..5666)
                     /gene="pfkA"
                     /locus_tag="ACGI6B_001706"
     CDS             complement(4707..5666)
                     /gene="pfkA"
                     /locus_tag="ACGI6B_001706"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFG9751152.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5875..9444)
                     /locus_tag="ACGI6B_001707"
     CDS             complement(5875..9444)
                     /locus_tag="ACGI6B_001707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFG9751153.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9601..10548)
                     /gene="whiA"
                     /locus_tag="ACGI6B_001708"
     CDS             complement(9601..10548)
                     /gene="whiA"
                     /locus_tag="ACGI6B_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFG9751154.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(10565..10990)
                     /locus_tag="ACGI6B_001709"
     CDS             complement(10565..10990)
                     /locus_tag="ACGI6B_001709"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9751155.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(11059..12165)
                     /gene="yvcK"
                     /locus_tag="ACGI6B_001710"
     CDS             complement(11059..12165)
                     /gene="yvcK"
                     /locus_tag="ACGI6B_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFG9751156.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(12177..13034)
                     /gene="rapZ"
                     /locus_tag="ACGI6B_001711"
     CDS             complement(12177..13034)
                     /gene="rapZ"
                     /locus_tag="ACGI6B_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFG9751157.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(13065..13778)
                     /locus_tag="ACGI6B_001712"
     CDS             complement(13065..13778)
                     /locus_tag="ACGI6B_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9751158.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(13782..14696)
                     /gene="murB"
                     /locus_tag="ACGI6B_001713"
     CDS             complement(13782..14696)
                     /gene="murB"
                     /locus_tag="ACGI6B_001713"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFG9751159.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14969..15739)
                     /locus_tag="ACGI6B_001714"
     CDS             complement(14969..15739)
                     /locus_tag="ACGI6B_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFG9751160.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            16057..17538
                     /gene="cls"
                     /locus_tag="ACGI6B_001715"
     CDS             16057..17538
                     /gene="cls"
                     /locus_tag="ACGI6B_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFG9751161.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            17856..18353
                     /locus_tag="ACGI6B_001716"
     CDS             17856..18353
                     /locus_tag="ACGI6B_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HFG9751162.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            18370..20256
                     /locus_tag="ACGI6B_001717"
     CDS             18370..20256
                     /locus_tag="ACGI6B_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HFG9751163.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            20279..22060
                     /locus_tag="ACGI6B_001718"
     CDS             20279..22060
                     /locus_tag="ACGI6B_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HFG9751164.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(22200..23996)
                     /locus_tag="ACGI6B_001719"
     CDS             complement(22200..23996)
                     /locus_tag="ACGI6B_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HFG9751165.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(24208..25137)
                     /gene="hprK"
                     /locus_tag="ACGI6B_001720"
     CDS             complement(24208..25137)
                     /gene="hprK"
                     /locus_tag="ACGI6B_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFG9751166.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(25137..26954)
                     /gene="uvrC"
                     /locus_tag="ACGI6B_001721"
     CDS             complement(25137..26954)
                     /gene="uvrC"
                     /locus_tag="ACGI6B_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFG9751167.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKVLTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(27213..30038)
                     /gene="uvrA"
                     /locus_tag="ACGI6B_001722"
     CDS             complement(27213..30038)
                     /gene="uvrA"
                     /locus_tag="ACGI6B_001722"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9751168.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(30057..32027)
                     /gene="uvrB"
                     /locus_tag="ACGI6B_001723"
     CDS             complement(30057..32027)
                     /gene="uvrB"
                     /locus_tag="ACGI6B_001723"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFG9751169.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(32214..33476)
                     /locus_tag="ACGI6B_001724"
     CDS             complement(32214..33476)
                     /locus_tag="ACGI6B_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFG9751170.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(33539..34843)
                     /locus_tag="ACGI6B_001725"
     CDS             complement(33539..34843)
                     /locus_tag="ACGI6B_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9751171.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(34874..35734)
                     /locus_tag="ACGI6B_001726"
     CDS             complement(34874..35734)
                     /locus_tag="ACGI6B_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9751172.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(35778..36647)
                     /locus_tag="ACGI6B_001727"
     CDS             complement(35778..36647)
                     /locus_tag="ACGI6B_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9751173.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(36631..37701)
                     /locus_tag="ACGI6B_001728"
     CDS             complement(36631..37701)
                     /locus_tag="ACGI6B_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751174.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        11911 a         6711 c         4702 g        14820 t
ORIGIN      
        1 gaacttaaat tctcttatct atgtgaaaat aaacatctct taaaatgaaa ttctgtaaat
       61 ataaatgatt ttttagtttg aaggattaga ttaacaaaat tataaatttg caaataagct
      121 atattaaaaa atataataat tttttatata acttattctt ttataacttt taattttaaa
      181 agaggagaat gtatataaga gtattcaatg tactagaaaa aatcaaaaga aatattagaa
      241 gaaatggata ttgagtaatc aaagaaaaga ttttgatatt acaaatgata tgtttcaaga
      301 aagcattcct atcacaacaa aaattattct tgaagacatg ccttcaaatg atgaattaaa
      361 tcatgttttt tcaaaaggtt gtgaacgaaa aatgaaaaag agaaaaattg tattaatcac
      421 actactatta ataggtgttt tgttattagg aagtatccta tataatcttt ttttaggaaa
      481 aacagctaat atttcaatgc taaaggaatc ttggaatttt gatataccaa tacctaacaa
      541 agaaattgaa gtatttgata ctcaagatag tattaatgga gatggacaaa gttattttat
      601 tcaaggattt tctgaaaaaa atttcaaaaa agtttttaat ttaaaaggtg gtatagttgt
      661 aagcaaagat aatattaatg aaatagaaaa atatattgat aaatttaaaa gagattctgt
      721 taacattaat aaaagtaata aaaataaaat tgaagaagat tttaaaaaat ataagttaga
      781 agttaaaaaa gatgataagt atatatataa gagaaattat gagaactacg tagttttaat
      841 tataaaaaaa gatgaacaaa aattatacag tttaatatgg aatcagtagg tacatatgta
      901 ttattaagaa ttataaataa taaaaataaa ttttagtcaa tatacaaaaa ccttttaaat
      961 tgtctggaaa tcacctcttc atttgttata aaaacataca acaaatgaag aggtgatttt
     1021 atggttttaa aaaaaataat tataagtgga tatgtgcttt cacatgttta tcaacatttg
     1081 taatgcctat atttgctaat gaattataaa aatattaaat aatattcaac tatagattat
     1141 gtaaattact ctatgaaaaa aatgagttgt ataaaatctg gctactgtag attgactatt
     1201 tcttttaaaa ttacaattat aataagtttt atagaattaa aaataaaact ttagagattt
     1261 ttgataacat tgtagactaa atttgccacc gaaatcgctt tacagtggca aatcggtggc
     1321 aaatacaata tattattagt atataatcta tataaacatt actattgttt gattatctta
     1381 gctacagttt ccacatgtgt agaatgaggg aacatatcta caggttgtgc ctcaacacat
     1441 ttaaatccgt gaacatttag ataatctaaa tcccttgcta aagtagaagg attacaagaa
     1501 acatagacaa ttctttccgg attcattgaa acaatagtct ctaatacctt ttcgtcacat
     1561 ccctttctag gtgggtctac aacgactaca ttggcagtct taccctctct atacatctta
     1621 ggtacaattt cctctgcctt tccaacataa aattcagcat tatgtatatt gtttaattta
     1681 gcatttatct tagcatcttt tatggcatca tctataattt ctattccata aaccttttta
     1741 gccttttgtg ccaaaaataa agatattgta cctatcccac agtaaatatc aaaaacagta
     1801 tcagtatcat ttaaatttgc atattcaaga gccttatcat acaatacttt agtttggaca
     1861 gggtttactt ggaaaaatga taatggtgaa atctcaaaca ctaagtctcc tatataatcg
     1921 tttatttttc catctccata tataactata ttttctcttc ctagaatagc attcgtatct
     1981 ttttgattta catttacaac caaagtctta aatccaggta tattttcctt aagcactgaa
     2041 gctaattctt taagatatgg aagtttgttt ccatttgcta ctaatacaac cattacttct
     2101 ttagttgtaa aacctatttt tgttactaaa tgcctaatca accccgtgtg agtcttttca
     2161 tcatatatgc ttaccttata ggcccttata tatgttttta ttattttaac aattttatca
     2221 tttatctcat gttgtattac gcatttttct gtcgatatta catcatgagt cttcttttta
     2281 tagaagccaa taattggaat accattattc ttttgtattg gaaattgtgc tttatttcta
     2341 tatctatatg gattatccat tcctatagtg tcatgaactt ctacatcctc taacttacct
     2401 attctcgaaa gtacttgttt aacttcattt gttttcattt caagttgttt attataatct
     2461 aagtcttgta tctgacaccc tccacaatcc ctaagattgt cactacacac tcttttcacc
     2521 ctgaatggag acttttctat tatctcaaca atatctccca cagcataatt tttctttact
     2581 ttattaatcc taactttaac tttatcatgt attaatccgc cctctacaaa aactgtaata
     2641 ccattgtact ttcctactcc tactccgcct tgaccaatgt ctactatatc tacaatatac
     2701 atctcgtttt tcgaaagcat aaatcaccct ccaatcaatc tattatattt aaatctttac
     2761 aaactttttt ctttaaattt atattaaaat attgctatta attaaactat attttttact
     2821 aagctacaat attttatttt accaaaaaaa aaggagctta taaagcccct tagtaaataa
     2881 atttattatt tttcttataa aactctagtt gaacctctat ataccacgcc tcttgatgca
     2941 tccacagtta caacttctcc atcttttact agatttgtta tatcagctgc agacacaata
     3001 actggtatat ctaaatttat acctacaata gctgtatgtg atgtcattcc accattctca
     3061 gttactacag ctcctgcttt ttctatataa ttgttcattt cagcatcagt agcagtagaa
     3121 acaagtatgt cgccttcatt aaagtctaca caagcatttc cattctttat tatacgaact
     3181 ttaccttcta ctgtttgagt acctacacct ataccttgca cgatttcttc gcttattaca
     3241 tgaaccttta ttaaattagt agttccactt actcctactg gtactccagc agttataact
     3301 actaattccc cattatctat ataatcagct tgtctagctg cctcaattgc cttttctata
     3361 acttcatctg tatttcctgc aacagaagac ttaactggat atactcccca tgtaagagct
     3421 aatttattca tagtcttttc attatttgta gtagctatta ttggagattt tggtctgaat
     3481 ttagatacca ttctagcagt atatcctgaa gaagtagatg ttattatagc tgatgcattt
     3541 aaatctactg ctgttgtaca agttgcatgg cttattgcat ctgttacagt aacattgtta
     3601 gtaccatttt cctttagtaa tctatcataa tctaaagtct cttctgttct ttttgctatt
     3661 gtagccatca tttttactgc ctctacagga tattttccag cagcagtttc tccagataac
     3721 attatcgcat ctgttccatc atatatagca ttggcaacgt ctgtaacttc tgctcttgtt
     3781 ggtcttggat ttcttatcat agaatcaagc atttgagtgg cagtaacaac tggctttgct
     3841 aattcattac atttttttat catcattttt tgaactattg gcatctcttc agttggtatc
     3901 tcaacaccta agtcacctct agccaccatt ataccatcag aaacttttaa tatttcatct
     3961 aggttctcta caccttcttg gttttctatt ttagatatta tttgtatatc agttgcatta
     4021 ttgttctcta atacttctct tattgctagt acatcagatg cctttcttac aaaagaagca
     4081 gctatataat ctattccttg acttattcca aactctatat cacttatatc tttaggagtt
     4141 attgctggta aatttatctt aacaccaggt acatttactc ctttatgatt tttaactatt
     4201 cctgaatttt caacaacaca aactatatct tctccattta tttcttttac tcttaagcca
     4261 actaaaccat catctattag tatagaatct cctgatttaa cgtcttcagc aagaccttta
     4321 tagcttactg tacacatttc tttagtcccc ataatatctt tcatagttat agtgaacttt
     4381 tgcccttctt ctaaaaatac ctctgggtct tcaaagttac ctgttctaat ttctggacct
     4441 ttagtatcta ataatatagc aactggctta tttaattttt ctctaacttt ctttgccata
     4501 tcaattcttt ctttatgttc ttcatgcgaa ccgtgagaaa agttaaatct acacacattt
     4561 agaccatttt gcataagttg agttaaaact tcctcacttt gtgatgctgg acctagtgta
     4621 caaacaattt ttgtcttctt tacattattt aacatatttg ctcctcctga gatattattc
     4681 cacagattta tttttaatac taaaacttat atagataata tttttgccat ctcataagct
     4741 tctttatcaa atactttctt ttgagctaaa gcttcactta tttctaaatc aattattttg
     4801 ttttctttta ttcctacaac tctagctgat tttccatcta ataataattc tactgctctt
     4861 actccaagtc tacttgctag tattctatca gatacagttg ggcttcctcc tctttggaca
     4921 tgccccaata cagtgactct taaatctgct cctgactctt ttttaagttc cttttctaaa
     4981 tcactagcac ttccgactcc ttctgctaaa acgattatac tatgtctctt acctcttttt
     5041 tgagtagtct ttaatcttag agcaacttca tctactgtaa tttctacttc tggtactatt
     5101 atagtttccg ctcctcctgc aagacctgca tataaggcta aatctccaca gtgtcttccc
     5161 ataacttcta ctatgttaac tctttcatga gatgatgatg tatctcttat cttacctata
     5221 gcatctataa tagtattcat tgcagtatca aatcctatag tataatctgt atatgctaaa
     5281 tcgttatcta tagttcctgg tatgcctata gctgggaatc ctaagtcact aagtttttgg
     5341 gctcctgcaa atgaaccatc tccaccaatt acaactaagc aatcaatttt atacttttta
     5401 agaattttta ctgcttttaa tctaccttct tctgttttaa attcttcaca tctggaagat
     5461 tttaatatag ttcctcccct gtgtataata tctccaacag aagataagtt catttcagtt
     5521 aaatcttctt ctagtaatcc cttatatcct ctattgattc catatacttt gcatccataa
     5581 tatattgctg atcttacaac cgctctaatc gcagcattca ttcctggagc atctcctcca
     5641 cttgttagta atcctatagt tttcattttt tacctccttg gattttatgt caatttttta
     5701 ttataatcat tgttattgta ttctaatgga ctttttttgt ccctatatca ctttttaatc
     5761 ccatcaagac taaaaaaatt aaatgtcctt aaatgtccat tttgtgatat tatatcacaa
     5821 aacggacaat atttttcata ttattataat atatttagat ttttatataa aaaatcaagc
     5881 ttttactttt atgttatctt taggaagtaa ctcttctaag ttatttataa attcatcaca
     5941 tatacaagtc aatgccccgg tcattttata tgctttacgt ttaccattta tattttcatc
     6001 catagggtat aagtatatac tgtcattacc tggatattgt ttggctattt ctacaatatt
     6061 attagttgta tcagtatcag acatactatc tattttcaaa tacaattttt taccactttc
     6121 tggcttaata ttttctacat ttctagttct tcctgaattg tagtttctat ctgcaaagtc
     6181 agtatcatca tttatatctt ttatctctct agctataagt tttgcatttt cgccttcttt
     6241 tatacttaat gttcctttta catatattat attatcttca ttcaaaatga cattatactt
     6301 ttgtaataat tgaggaaaaa ctattatttc tatagcacca tacaaatctt ctaattcaat
     6361 aaatgccata atttcatttc gttttgttgt tttaatagtt ttagttacaa tcattccacc
     6421 cattataaca tctgtttcat tcatttgtag gtatgactct tcttcctctt ttaatgaatt
     6481 taattttcca ttatccatag atgttttttc ttgtagttct ttttcaaatt gtgataaagg
     6541 atgtccactt acatacattc ccaagacttc tttttcaagg ttaagtctct ctctttcttc
     6601 gtactcttgc cttttaggca aactgtatat ttgttgaaaa tcactatctt cctcactatc
     6661 tccaaaagca tcaaataaag aaatttgacc ttgaacattt ttctttctat ccatagatac
     6721 actttctaat aaactttcat aaccagccat cagactagct ctattttcac ttatattatc
     6781 aaaagctcca cattttatta gactttctat tactctctta tttgtatctt tttggtctaa
     6841 tcttttgact aaatctacta aatctacaaa taatccatta gattctcttt cagcgattat
     6901 attgtttatt atatttacgc caacattttt tacggctgct agaccaaatc ttatattgtc
     6961 gccttcaact gaaaactttg aaaaactttt gtttatatct ggaggtaata catctatttc
     7021 tagtgcttta cattctctta tatactcaac taccttatca gtatttccca ttacactggt
     7081 tatcaatgct gccataaatt caactggata atatgttttt agataagctg tctcatatgc
     7141 taaaacccca tatgcagctg catgtgattt attaaatgca tatctagcga agtctatcat
     7201 atcatcaaat attttattag ctatttcctc ttctacacca tttctgatac accctgcaat
     7261 ttctatatta ccatcttcat caaatttacc atgaataaaa tattgtcttt cttcttccat
     7321 cacgtccatc tttttcttac tcatggctct tctaactagg tcacttcgtc cgtaactata
     7381 accacctaaa tctctaacga cctgcataac ctgctcttga tataccaaac aaccatatgt
     7441 aacttccatt ataggcttta atttttcatg tagatatgtt acatcttttg ggttgctttt
     7501 attttttata tatgcaggta tagaatccat tggacctggt ctaaataggg atattcccgc
     7561 tacaatatct tcaaaattgt ctggttttag ctgtttcata aaacttctca tgccagcact
     7621 ctccaattga aacaccccta gtgtatttcc tgatgaaagc atttcatata ctttcgggtc
     7681 gtcataatcc atctttgaaa agtctatttt ttctgtgtaa ttttttataa ctctattttt
     7741 ttctattaaa tcaagagtat ctcttataac tgtaagagtt cttagcccaa gaaaatccat
     7801 ctttaaaagc ccaagttctt caagtgttgt catagtaaac tgtgttgtga ttgcatcttg
     7861 atgtttgtac aaaggtacat attcatctac gggatttttt gatataacaa ctcctgctgc
     7921 atgagtagaa gcatgtctta gcattccctc tatctgtttt gatatattta ttatttcttt
     7981 tgtttccggg tcttggtcat acaattcttt aagattagga tttgtatcta gggccttatc
     8041 tattgtcatt cctaaagcaa atggtatttc cttggctacc ttgtcaactt tattgtagcc
     8101 tatatccaat acccttccaa catctcttat tgcagctttt gcacccattg ttccaaaagt
     8161 tattatctgg gctacatggt catcaccata ttttctcttt acataatcta taacttcttc
     8221 tcttctctca taacaaaaat ctatatctat atctggcatg gatatacgtt ctggatttaa
     8281 aaaacgctca aataatagag agtactttat tgggtctata tcagttattt taagtgtata
     8341 tgctactatc gaaccagccg cactacctct acctggtcct actataatat tattttcttt
     8401 agaaaaattt ataaaatccc aaacaattaa aaaatactct acataaccca ttttttcaat
     8461 tacatctaat tcataattta atctctctag tatctcatta ctaggattat catatctctc
     8521 ttttaatcca ttaaaacaca actcccttaa ataagtttct ggtgtatatc cttctggaac
     8581 atcatactta ggaagatgta tagtattaaa atcaaactcc acattacaca tatcagctat
     8641 tttcacagta ttgtcaatag cctctaatgc atatggaaat aattcttcca tttcttctct
     8701 tgattttaaa taaaactcat cactaccaaa tctcattcta tttgggtcat ttaatgtttt
     8761 tcccatttga atacacataa gtacatcatg tattttcgaa tcttctttat ttacatagtg
     8821 aacatcatta gtagcaacta gtggtatacc agtctctttt gataatttta ctaaaccaga
     8881 gtttacttct ttttgctcag gtaaattgtg atcttgaatc tccaaaaaga aattttcttc
     8941 tccaaatatg tctctgtagt caagagcaaa ttgttttgct ttttcataat ttctattcat
     9001 taaagcacgt gctacatctc ctgcaaggca tgcagaaagt gctataatcc ctttgttatg
     9061 ttttttaagt tcttccatgt caactctagg cttatagtaa aaaccttcta cgtatgaagt
     9121 tgaaactatt tttattaaat ttttatatcc ttccatattc ttagctaaca gaactaaatg
     9181 tccttgatat ttgtcatagt ttgggtcttt atctctcaat ccacgtgcag ctgtatagac
     9241 ttcacaccct ataattggct ttattccttc ttttatagct gttttgtaga aatctataac
     9301 accaaacata caaccatggt cagttatagc tattgagctc atgtttagtt ccttagccct
     9361 ttttataagt tttttcaccc tcgaaaaccc atcaagtaaa ctatactcag tatgtacatg
     9421 aagatgagca aaatctttgt tcattaaatc acctcctgct ttataatttt aattttacca
     9481 caaatatatg tcctaaaaca aaaacagaga cttttatctc aaaataggaa ttaacttcaa
     9541 cgtataccat tatatgttat aaattaatgc ttattctgag attgtcccct caatttattg
     9601 ctatgatttt tttttcaaat ctgttgcaat ttcttctatt tttctaagtc tatgattgac
     9661 tccagatttt cctattggtg gttctaacat ctctccaagt tctttgagag tcatatcctc
     9721 atactcgatt ctaatcttag ctatctcttg taaattttct ggcaaactgc taattcctat
     9781 agtctcttgt atgagcttaa tattttctac ttgtcttact gctgcattta ctgttttaga
     9841 aaggttagct gtctcacaat ttactatcct gtttacatta tttctcattt cttttactat
     9901 tttagtattt tgaagactta aaagagcgtg gtgtcctcct ataatactca acaaatctga
     9961 tatttgttca ctctctttga tataaacaac gtgattattt tttctagcca caattttact
    10021 attaaaacct aaagagttta taagttcttt tagagaatct gcaaactcct cattattagt
    10081 tacaaattcc atatgatagt tcttttctgg gtcgcttata gaacctccac caagaaaagc
    10141 tcctcttatg aaagctctct tacatccttc atgcttaatt aagttctctg aaattttgtt
    10201 tatagtaaaa aatccttctt tatcttcaag tatacctaac ttttttaata acatttctga
    10261 acccatttcg cttgttacca ttaatacata actgttattt ctctttagca tttgattctt
    10321 atttactgag attgtagtat tgatattaaa attctttttt aataatttaa atactttacg
    10381 ggctattgaa tttaactctg ttgttatctt tagatttagc ttcttatatc caacaatttg
    10441 tatacttccc cctgatttaa ctagagctga caattcagct atattacaac attcattccc
    10501 agatataact cttgctagtt catttttggt ttcagttgaa aaagacatat ctatcaccta
    10561 ttaactaatt tttctttaac ctttttttta tttcttgtaa ggcaaccttt attatctcct
    10621 tttcatcatc atacctagct aagtctacta ctctatctag gtggataaat ttagcatcaa
    10681 taaagccttc ctctttttgt ggaatagtat ccatattctt tgcttgcatc aaataccaaa
    10741 aaactgtttt atgaactgct ctattttcat cccagttttc tttgaatgta tagtgtattt
    10801 cacctaaata tttcaatata tctgctttta ctccagtttc ttcacttact tcacgtaatg
    10861 ctgcctcttg gttattttcc ccttcttcta cttttccctt gggtaacacc caatctccat
    10921 tgaacttcct tagtaaaaga atcgtattgc caaacaggac tacaccacca gcactaacct
    10981 cttctctcat cgatgttcac tccttaaaac taacataatg ataaggattt ctacacaatt
    11041 ttttaaaatc cttttttact aacttttgtt gtaattatga tttagggcta actcaataac
    11101 tatgttagat atatactttg catcatgtct tatataatta ttttttattt caattaaatt
    11161 cttctctaca gtttttatac ccatctcttt taatttatct ttttgctttt tatctaacaa
    11221 gacttgctct gctccatctt gtttatataa gtcaagcatt tcttctggca atatctcatt
    11281 atttgcgatt acataatcta ttaaattctc atcagtgtgt tttactatag cattaacatg
    11341 ctctaataca ttgtaccctt ctgtctcacc tggttgagtc attatatttg ctatatatac
    11401 ttttggagct acagacgatt taatagcgtc tacaatcccc tctacaagta aatttggtat
    11461 tatacttgta tataaacttc caggacccat aattataatg tcagcatcat aaatagatgc
    11521 aataacatcc tttaatggtt ttacatcttt tggatttaaa aaaattttat caatagagct
    11581 tttttgaatt tttgactctt caggtatact agattctcca tttattatat tgccattttc
    11641 taatttggca actaaattta catcttccaa agttactggt aaaactcttc ctgttatagc
    11701 aaaaatttca cttaatttat atacagcttt ttcaaagttt ccatacaacc cattcatagc
    11761 agccaaaaac aaatttccaa aactctgacc ttttaaaagc ccttctgtaa acctatattg
    11821 cataacttca ttcattgttg gctctatatt tgcaagtgct aataaacagt ttcttatgtc
    11881 tcctggtggg agcattccta agtcttctct caatacacct gaacctccac catcatcagc
    11941 aacagttaca attgcagtta tattttgcgt agtgtgttta agacctctta aaaataccga
    12001 ttgaccagta cctccaccta tcacaaccac atttggttct ctattttcaa caatagacct
    12061 ttgaaatagt tttacttttt cattttttgc ttttttatat ataaataacg atacaattaa
    12121 tgcaatccaa cctaaaattc ctacaataat aagtaattta gccatgtttg cctccattat
    12181 cgcaacatag agtctctatg tttctttaca actctatatc ccttattaga taaatcctcc
    12241 gctataagat ttgttatagt aactgaccta tgtcttccac ctgtgcatcc aacaccaatt
    12301 actagatgtt gttttccttc ctctatatat tgaggtacta aaaaatgaat catatctaaa
    12361 agtttcacat aaaattcttc actgatttta gaattcataa cataatctct aacttcttta
    12421 tcatcaccag ttttagctct taactcttca acataatagg gatttggaag aaatcttaca
    12481 tcaaatacta aatctgcatc agcaagtata ccatgtttaa atccaaaaga tactacagaa
    12541 atagttaggt ttggattatc ttcaccagaa gaatatattt tctttatttc ttccttaagg
    12601 tctttaggtt tcatgttagt tgtatcaatt atacatgtag ataactcctt taaaggttcc
    12661 attatctttc tttccatttt tataccttct ggaatctgca tatcttttgc caatggatga
    12721 tttcttcttg tcattttata tctctttagc aatacatcat cattacaatc caaatatacc
    12781 atttcatatt cataattttc tttttctaaa taatttaagc tttcatgaag cgcttcaaaa
    12841 aactttctcc ctcttatatc tatccctaga gcaactttat caatacttga atttggttga
    12901 taacataatt ctgcaaattt agttatcaaa gcaggaggta gattatctac acaataaaat
    12961 cccatatcct ctaaagctct cattgtctca ctttttccag atcctgaaag acctgtaact
    13021 ataacaaatt tcattgctaa ctcctccctt ttatcttatg tttattatat tataatgttt
    13081 tctactaacg atatatccag attagagtca gatatagtct tcatagctag ttcaaaatca
    13141 ccaactcttt ctggacaatg tgcatctgag cctattataa atttattttc tagccctttt
    13201 attttattta attgatttac attcaaaaaa gaatggctac tatttatctc aagtctagta
    13261 tttgtctttt ttgctgtctt tgctacttct tctatataca catcgccttt atctcctgga
    13321 tgagtaatta taaaaatatc attattactt atcgcattta ttacagcttt tgtattatat
    13381 tcttttgctt tttcagtttt aaaaatatag ttattacaat gatttaataa gcttcttaat
    13441 gatgttggag tagaaccaaa atgatatcca gccataatat aatcaaaatc tctaataaat
    13501 ttatcatcca tatctatatt tcctttatca tctaatatgt tacactccat acctaaaagt
    13561 atatttatat cagtgtactt atcatttaaa taatctactt cttctctcat tttgagaata
    13621 tcttttttct ttacaccata tgcaatatgt ttataactat ggtcagatat tcctattgtt
    13681 tttattcctt tagaaatggc tacctttaca ttatcctcaa tagtcccttt gccatgacta
    13741 tataatgtat gtgtatgata atcagccagt attttcattt gttaatcctc tccaagtatt
    13801 ttgacttctt cttccaacat aaccccaaac ttggcatata cagcactttt tataacatat
    13861 attaaatcca aaatatcttt agcacttgct tctccttgat tgactacaaa tccacaatgc
    13921 ttctcagata cttgtgctcc tcttaaagta agacctctca atccagtttc ttctattaat
    13981 ttacctgcaa aatatcctgt tggtctctta aatgtacttc ctgcactagc aaaatttaat
    14041 ggctgtttag ttattcttct cgtcgctaaa tcatccatta aactttttat ttcatcataa
    14101 tttccttctt gtaattctaa ttctgctgat aaagctatat atccgttttt cgatattata
    14161 ctttttctat acccaaattc catctgttca tttgataatt caaatatatt gccttccata
    14221 tccatcagtc ttactgactt cactatatcc ttcatttcac caccatatgc tccagcattc
    14281 atggctaatg ctcctcccaa tgtgccaggt attcctgatg caaactcaaa cccttttaat
    14341 tcttgctttt ggagagcttt ccctaaaact gataataaag ctcctgactg agcagtcatc
    14401 ctagtatcat ttatctcata agaattgaaa ttatcagcta attctattac cactcctctt
    14461 attccaccat ctttaattaa aatgtttgaa ccatttccaa ttattaaata cggaatagac
    14521 tcttttttta ccaacttaag tacatttttt aattgttctt cagttctagg tctaactaat
    14581 atatctgctg gtcctcctac cctaaatgaa atatgttttt tcataggttc atcgactttt
    14641 atatcctctt tacttaatat atttaataaa tttatatata tatcttgatt attcattgta
    14701 tttcctccca aattatactg aactttagct gtcattatta tttaaattac attaatatat
    14761 agtattaact atattcaata atatatgatt ttgcttatca tatggatttt atattttaag
    14821 cttcttaatt acaaactata ttcaataaat atataataaa gtaaaaaaaa aaaaataaca
    14881 acatataaaa gaatataaat aatttaaaaa aagcatcaat atcactgata tcaatgcttt
    14941 aaaaggcaat ttaattcgta ttatttatct aactattttt gacatagcaa agataataaa
    15001 tccctccaac tactatggaa cctccaacga tatttccaag tgttacaaat accaaattag
    15061 ttataaattg aaccatagtt atatttgctc ctaatgccat tcccattggt ataaaaaaca
    15121 tgtttgcaac actatgttgg aatccacata atacaaatag cattattact gtccaacatg
    15181 aagccaattt tgacattata tcttgagatg ctgtagccat ccatacagct aaaacgacta
    15241 gaatattaca aagtattgct tttaaaaaca ttgatgctat agttgaatta accttagctt
    15301 ctgccacagc tatagctttt gtagctgcat ctcctgtcaa tgtagaagaa taaaacacaa
    15361 gtgcaactag caaaattgaa cctactaaat ttgcaaaata aaccagtccc cagtttctta
    15421 acatttttcc taatgttatt tttttgtcca taagtgcaat agccattaaa gtgtttccag
    15481 tgaataattc tgctcctgcc actactacaa gcataagacc tactggaaag accatagcac
    15541 ttaaaaagct agctacccct gggtctatct tgccaaaagt ttgactaact atcatactcc
    15601 ccaatgcccc aaaacctata aatgctcctc caaataaacc taatagaatt gcattttgtg
    15661 tcgaaatatt tgctttttta atgcctgcac ttatagttgc ttgtgcagtt tccccaggtg
    15721 tcaagaatcc tttactcata aatccttccc ccttaaaaat tatactttcc cttgtctttt
    15781 tatggcaata attatataat aacaatatga tatataataa attttattaa cataaaaaat
    15841 atactatata cttatatata cttgtctcat ataattatac tatttattat ataacaactt
    15901 tgttaatttc gaaataattt atattgaatt tgaaaaattt accttttgat tatttatttt
    15961 actttttaat tattgaggta ttatatataa tagatatatg tttgtgttat tatatataat
    16021 tacttatttt aataaatatt tgaaggaggg aattttatgt ttgatatatc atttttcgaa
    16081 atgtcaatat atgaaatagc agttactaca atatatataa ttaatttcat ggttatacta
    16141 aaccttatct ttagagaaaa aagaaacata aatacaactt taacatggct tcttatatta
    16201 gtattgattc ctgctcttgg attcatactt tatatagctt ttggcagaaa tatttcaaaa
    16261 aacaatatgt ttagattgaa agaaaaggat gataagataa tcaaaagtaa tatactagat
    16321 actcaggtta aactccaatc tacatctgaa attgattcag atatacatca acataaggat
    16381 atgatttatg cattggctaa ttcaaataat gctcattata ccaataataa cgatgtgtgg
    16441 atttatgctg aatctagtca attttttaac agtcttcttg aagaacttaa aaaagctaaa
    16501 aaatatataa acatacaatt ttacattttc aaagatgaca aaatcggaac tgaaataata
    16561 gacattctta tagacaaagc taaagaaggt gttgaagtaa gacttttatt tgatgcagtt
    16621 ggtggtagaa cactaaaaaa tagtacactt tccagactta aagaaagtgg tgttaaagtt
    16681 ggaagcttct tcccatcatt cttgaaaatt gtaaacttta acttaaacta tagaaatcac
    16741 cgaaaaattg ttgtcattga tggtaaggta ggatttgtag gtggatataa tgttggtgat
    16801 gaatatttag gtagaaatcc aaaatttggt ctatggagag atacacatac taagcttact
    16861 ggtgattgcg taattgacct aaatatgaga ttcatccttg attggagata tacaactaaa
    16921 gaagacttag atttagaaaa atactttata gaagaagata caaattctaa tcatccttct
    16981 gaaaatattg ggattcagat agtatcaagt ggtccagata tatcagaact tgatgagatt
    17041 aaatatggtt accttaaaat gattcagaaa gctagaaagt atatatacat acagagtcct
    17101 tatttaatac ttgactcaac ttttatagat actttaaaaa tagcctgttt atctggtgtt
    17161 gatgtgagag tgatgattcc ttctaaacct gaccatccct ttgtatactg ggcatcatat
    17221 tcatatgctg gagaactttt aaagtttgga gctaaaatct atacttatgg tcaaaatgca
    17281 tttttacatg ctaagacaat agttatagat gactctatat gctctatagg aacagctaat
    17341 atggatatca gaagttttga gcttaatttc gaagttaatg catttatgta ctcttcaaaa
    17401 aaagcattgg aacaaagaat tatatttgaa aatgatattt taaattctaa ggaaataaca
    17461 ttagatatat ataattctag gtctacttat ataaaaatca aggaatcaat ttcaagacta
    17521 ttatcagcag ttttataaat cttttatttt ataaaaattt tacattttgg tttatttttt
    17581 aaagtttttt atatgtatta tttttttttt attgaattta gctcattttt tttatttttt
    17641 ttaactattc tgaattattt tatattataa tagactagta tagtttttat ttttaatagg
    17701 taactttttt gcattgtttt tataaataaa gaaattgttg tatattattc tttctttatt
    17761 gttttggttt tgttcaccca cataatgttg gggtcaactc atggttaatt attttatgaa
    17821 aaataataac tttaaaaaga aaagggggat ttgttatgtg cgattttatt tcacacaaca
    17881 agcaattatt tgacgaacta gatatattta ttgactctct tgcaacaaag gaaggtgcgt
    17941 taatacaagt tctacatgaa gcccaaggta tatttggata tcttccaaaa gaagttcaac
    18001 ttcatgttgc tagaaaactt ggagtagctc ctgcaaaggt atatggggtt gttacattct
    18061 attcttattt tactacagaa cctgtaggta agtataaaat cagtgtctgt ctaggaactg
    18121 tatgttttgt aaaaggtgct gacaaaatct taagtgcttt tgaaaaacag cttggcataa
    18181 aagttggaga aactactagt gattttaaat tttctttaga aggtttaaga tgtttggggg
    18241 catgtggttt agctccagtt gttactgtaa atggtaaagt ttatggtaaa gtgaaacctg
    18301 accaagtaag cgaaatatta gatacttata gagaattaga attgaattgt taagaggggt
    18361 gaataaataa tgcgtaaagt aaattcattt gatgaattaa aagttttagc tgatgagctg
    18421 aaacctaatc ttagcttaag aaaaaattat tctaaggaaa atgtattaag aagagaatta
    18481 cttgtttgtt gtgatactgg atgtacatct tcaaacagtc tagaaatagt tagtgaatta
    18541 gaaaatgaaa taaagaaatc aggaatccaa gataaagtta gtgttcgttt gacaggttgt
    18601 tttggtttct gtgctcaagg tcctattgta aaagtttatc ctgataacgt tttctacgta
    18661 aaagttgagc catctgatgc tgaaaagata gttcaaagcc acttaattag aaatacagtt
    18721 gtcgaggaat tattatatga ggaagaatct ttaagtaaaa aagttgaaga acaagaagaa
    18781 atgtctttct acaaaaaaca gttacgtata gctttaaaaa attgtggtct tatagaccca
    18841 gatagtatag aagaatatat tgccaatgat ggttatttag ctctaggcaa atgtttaact
    18901 tctcttactc ctcaagaagt tattgcagag gtaaaaactt ctggccttcg tggtagaggt
    18961 ggtgcaggtt tccctactgg ctctaaatgg gaagctgctt caaaaaatcc agctggtcca
    19021 acagataaaa agtttgttgt atgtaatgct gatgaaggtg acccaggtgc atttatggat
    19081 agatctgtac ttgaaggtga ccctcatagt gtactagaag ctatggcaat atgtggatac
    19141 gctataggtt ctgatacagg atacatatac ataagagcag aatatccaaa atctatagaa
    19201 agattaaaag tagccatagc tcaagctgaa aaatacggtt tacttggcga aaatatctta
    19261 ggcactggat ttaactttaa attagaatta aaatatggtg caggtgcttt cgtttgtggt
    19321 gaaggtacag cgctaatgca ttcaattgaa ggtagacgtg gtgaacctag aatgaaaact
    19381 tatagttctt ctaagagtgg tctatggaaa tctcctactt gcttaaataa cgttgagact
    19441 tttgctaaca tacctgcaat tatacttaag ggtggagatt ggtttgccaa cttaggtact
    19501 gaagattcta gtggtactaa agtctttgct cttggtggaa aggtagaaaa tgtaggactt
    19561 gttgaagttc caatgggtac tactttaaga gatattgtat ttgaaattgg tgggggaatc
    19621 cctgaaggta agaaatttaa ggctgttcaa acaggtggac catctggtgg atgtatacca
    19681 actgaacatt tagacactcc tatagacttt ggttcattaa gttctatcgg ttcaatgatg
    19741 ggctctggtg gtatgcttgt tcttgatgaa tctgactgta tggtagatat agccaagttc
    19801 ttccttgagt ttactgttga agaatcttgt ggtaaatgta caccttgtcg tataggtact
    19861 acaagattat tagaaatatt aactaaaatt acagaaggta atggtactct acaagattta
    19921 gatgacctag aaaaccttgc tgaaactata caaactgctt cattatgtgg tcttggtaaa
    19981 gctgctccaa accctgtttt aagtactctt caatatttca aagatgagta tatagctcat
    20041 gttgtagata agaaatgtcc tgctggtaaa tgtcagaact tactttctta ctttataaca
    20101 gatgactgta agggttgtac aaaatgttct agagtgtgtc ctgctggttg tataacaggt
    20161 tctgttaagg aacaacatac aatagatact tctaagtgtc taaaatgtgg tgcttgtata
    20221 gataattgta catttaacgc tataatcaag aaataactta tttggaggag gaataacaat
    20281 gagtttagtt aatttaacta ttaatggcaa acacgtttca gcacctagtg gaacttctat
    20341 cttagatgct gcgaaattaa taaatataaa aatacacaac ctttgtcatt tacatatgaa
    20401 tgaaatagat aagcttgaca cttgtgcttc ttgtcgtgtt tgtatggttg agacagagag
    20461 aggattagtt ccagcttgtg gaacagttat aaaggaaggt atgaaagttc agactaatag
    20521 tgctaaagca ttaaatgctc gtagaactat tgttgaatta ttgttatcag accatccaca
    20581 agattgtttt gtctgtgaaa aaaatggaga ttgtgaatta cagactatag ctgctgattt
    20641 aggagtaaga aaaataagat atcaaggttc taaatctttt gcaggtaaag atacttctac
    20701 taaatcttta gttaaagacc attctaagtg tatattatgt agaagatgtg agactgtatg
    20761 taatgacata caaacagtag gagcattatc tggagttaat cgtggattta atactttagt
    20821 tagtacattc tttaactctg atatggttga aacagagtgt acattctgtg gtcaatgtat
    20881 atctgtctgt ccaactggag cattaacaga agtagataat gtacctaaac tttgggatgt
    20941 tttaaataaa aaagaaaaga ctatagtagt gcaagttgct cctgctgtaa gagttgctat
    21001 tggtgaagag tttggattag aaccagggtc aatctctaca gggaaaatgg tagctgcttt
    21061 aaaagcactt ggatttgaac atgtatttga tacaaacttt ggtgcagatt ttactataat
    21121 ggaagaagct actgaattta tagaaagaat ccaaaaaggt gaaaatcttc ctatattaac
    21181 aagttgttgt cctgcatggg taaacttctt agagcacaat tatccagaca agttaaactt
    21241 agcatctagc tgtaagtctc ctcaaggtat gtttggttct atagctaaaa actactatgc
    21301 acctaaaata ttaggaatca atcctgaaga actttatgtt gtatctgtaa tgccttgtgt
    21361 agctaaaaaa tatgaagctt caagagaaga attaagtgaa tctggaatac tagatgttga
    21421 cctttctata actactagag agttagctaa gatgataaaa gaagctgcaa tcgatttacc
    21481 taatcttcaa gaccaagatt ttgataatcc tctaggaaaa tctacaggtg ctgcatctat
    21541 atttggagca agtggtggag ttttagaagc tgccctacgt acttcatatg agaagataac
    21601 taataagact ttagataatg taaacttcac taatgttaga ggtcttaaag gaattaggga
    21661 agcaagtata gatgttgatg gaactacagt taatgtatgt attgtaaata cacttaaaaa
    21721 cgctagaaag attatggata aggtcagaag tggagaatgt aaataccata tcatagaagt
    21781 tatggcatgt cctggtggat gtgttggagg tgctggtcaa ccttatcacc atggtaatac
    21841 tgaaatagtt gatagacgtg caaatgcgtt atatgaaata gatagaaata aggctattcg
    21901 taaatctcat gagaatcctg atttacaagc tatttataaa gacttctttg gagaaccaaa
    21961 tagtgatgta gctcataaat accttcatac tcattacttt gataagagtt gtgtctatgg
    22021 agagtgtcca caagaatgtg cttgtgaaga agctaagtag tcataacaaa gtataaaatt
    22081 tattcttaaa aaaggattgt cttaaaatac ttttacgtaa tttaagacaa tccttattaa
    22141 tttcacaaat taaattatat ttctaaatag acttatttat taattaacct atctattaat
    22201 taaagaatac ctgccattct cataagttgt tttgcatttg tagttttaca tcttccatct
    22261 tttatcttat agtcaaaatg aattttatcg tctacatagt actctgagaa atgatagttt
    22321 acaacttcaa catctccgct acctgaaagg tcacatagct caaagtcatg tgttgtcacc
    22381 atacttataa cccagggttt tgataatttc tttactgcct cacttgctcc aataattcta
    22441 tcagcagagt tagttccctt aaaaatttca tctactagaa ccaacattgg cagttctttt
    22501 aaactatatt gaatcattga ttttattcta agaatctcag catagaaagt tgatattcct
    22561 tgacttacat catcttgtat tctcatagaa gtaaatatag ccatacaggt cgcatcaaaa
    22621 ctcttagcgc atactggagc acctgcatat gccagcacta agtttacacc gatacttctt
    22681 aaaaatgttg tctttccaga catatttgaa cctgtgataa tacaagtctg agaatttaat
    22741 tgtatcgaat ttgctatgac acttttttct gctattaatg ggtggtatac ctcttcaact
    22801 ttcaatcttg gagtatcttc atattttatt actggaaaac atgtattttc ctttacatga
    22861 gaaacaactg ttaaacttat tagagcttca aattctccaa tagattcaaa ccaatctcta
    22921 atttgatttc catatatacc tttccatcgc tccaaagctt ctgcacaatg atagttccac
    22981 atcaaaacac cacaggcaat tatatatagt atctgattat accttaggtt tacagcattt
    23041 cctattgaat ttaattgttt aacacctctt gaaactccac ttcccttaga aagtttatct
    23101 tgtaattctt tcaaatatgc actctcaaac gtctcttttt ctaaaacctc aaacattttt
    23161 tcatatactt gtatacttct actaaatgaa aataatggag caagagtttg cataagtctt
    23221 ggatatccaa aaccactaaa ccctaactgg attataaaaa gtaaaaaaac aggtaaaaca
    23281 ggtaaaaatc ctaacataca aaatataaaa gataagattg ttgcagttgg taaaccccaa
    23341 gtaaaaatat gcatccattt tggtatatac acattcttat tttctccata ttctataaac
    23401 gattctatac tactatctga attgtttttt tgctctttac caattatatt actgagtgtt
    23461 tgaatatgaa tagaaaaatc atcttttgat aaaagttctt ttatagctct ctgtctttct
    23521 aaaattatat tctcatctgg attttcattc cacaaatatt tggctaaaaa atttttccca
    23581 taagatgtat tagctacaca gatatattga tacaatgatg ctcttccaaa taagtctaaa
    23641 tcttttgctt gcgaattttc atcttttaag tattctttac catctatttt aaattccttc
    23701 caaccatctc caaatctctt aatatactta tttaatactt cgctcttact tttaaaatat
    23761 gtttcttctt cttttacttt actatgaata actaagagtg ctataaatat tattattgat
    23821 aaaactccaa caaaaatacc tactatattt ttattataaa atccaatagc aagccctgct
    23881 atcactatca aaaaagatat taatcttgct gtacttatgt tatttgactt tttacttaag
    23941 agcaattctt tttcagtact actttctaaa agtttactaa aatattctat tcccataaaa
    24001 aattccaact ttcttgttat ataatgaaac tcaaatataa aattaatcta gtattattat
    24061 aatctaacac tgtactttaa attatcctta tataatatat cttattaata aacattatat
    24121 atacaacttg attttaattt ataaggtgta ggacataatc ctaatcaata tttaattaaa
    24181 tgatgtgtag tccactatta ttcagtacta cttagtatcg attaattttg ataatttttt
    24241 agtaaattct tgagctgcat cgtatcccat tcctctttgt ctaaaattca ttgctgctac
    24301 ttctaatatc atagcagtat ttcttccagg tttaacaggt acaactaatt tctctacatt
    24361 aacaccaagt atttcttcat attctttatc cagtcctagt ctatcataat acttagtttc
    24421 atcccaagct tctaaatgaa ctactaaatc tatcatttta gaatttttga ttgcccctac
    24481 tccatataaa cttctaacat ctattattcc tattcctctt atctcaagaa aatgtcttat
    24541 tagttcaggt gactgaccca caagcatact ttcatctaat tttcttattt ctacagcatc
    24601 atctgcaact aatctattac ccctttgaat cagttctaat gctgtttccg actttcctat
    24661 actactttca ccttttatca gtacacctat accataaata tctactagca caccgtgaat
    24721 tgttgtatgt ggtgctaact tattatccaa atagtttgac agtctattta taagtctagt
    24781 ggtatttcta tttgtgctca aaagtattct tttatatttt ttagcctttt caattacatc
    24841 tggcttgatt tccaagtctc tagaaattat taacgctggt atttcatatg agaaaaatct
    24901 atctagtact tcttcccgaa tatgactatc cattaatcca aaaaaattat attctgtctt
    24961 tccaactatt tgtattcttt catatgcaaa ataatcaaaa tatcctgcaa actgtaatcc
    25021 tggtctattt atatcttgag agtagacata ataatctaca tcatcaggca tgtatactac
    25081 ttttaacttc aactctttta ttatctctct tattgatact ttattgctag tttccattaa
    25141 ttttcttcct ttctaaaaaa attataaaca ttatctgcaa tgcttttatt cattccatct
    25201 acttctaaaa gttcttccat agtagctttt tttatagcat ctacatcttt aaagtgattt
    25261 aataatgatt tctttctttt ttcacctatt ccttgtatat catctaagat agattttgtc
    25321 aaaactttat ttcttagact tctatgatat gttattgcat aattatgaac ttcttcttgt
    25381 atactagcaa taaatctata taaattagtt gttttatcta gttctatttc tttttctttg
    25441 catatcaatc ctttagttct atgcttatca tctttgtaca ttccccaaag aggtatatct
    25501 acatcattga gttctagtac tttttttact gcactaactt gtccttttcc accatccaac
    25561 aatatcaagt caggtaaatt tccatgtttt aatcttctat ctacaatttc agccatagaa
    25621 tcataatcat taggtccaat aactgtcttt attttatatc tcctatattc cttcttatct
    25681 tttttagcat tcgtatacac taccatagaa ccaatcgaat ctacaccttg tatatttgat
    25741 atatcataag cctctattct tattggcaac ttttctagat ttaatatttg ctttaattct
    25801 tccaatgctc caatactttt ttcatatttt cttttattca tatcagaaaa tttttcaaga
    25861 tattctacag catttttcct aaccatttca actaagcttt tcttctctcc cttttgagga
    25921 actcttattg ttactttttg ccccttttta gatgacagcc attcttctaa tatagaacta
    25981 tcttctatct catcttctat aattaattct ttaggtatat actcttgttc attataaaat
    26041 tgttttacaa atgaacttaa tatagaagct cttggactat ccattacccc ttctaatata
    26101 aaatgttccc tacctacaat cttaccattt cttataaaaa atacctggac acaagcttca
    26161 ttatgagctc ttgccattgc aaccacatct tggtttaagt cacttacagt agcatctatt
    26221 ttttgtttct gtatcatctc ttcaaggctc tttatcttat ccctatatac agcagcctct
    26281 tcaaatctaa agttcataga actctcattc attttttcct ttaaaagttc catcaatttt
    26341 tcttctttac cagataaaca cattataatc tcttctatca tttttccata ctcttcttta
    26401 gaaacatttc ctgtacaagg tcctaaacat ttatttatgt gtagatttaa acaaggtctt
    26461 gtcttattct ttattgcttt atctatatct attttacagc ttcttattgg atacgtacta
    26521 cttataagct ccaaggtatc attcacagca gttatattgg tatagggccc aaaatacttt
    26581 gccttatctt taagaactct tctaactttt aagattcttg gataatcttc attcgtagtt
    26641 acttttatat atggataagt tttatcatct ctaagcacta cattatactt tggtctgtat
    26701 ctttttatta aattacattc aagtattaat gcctctaact ctgaatccgt tataatatac
    26761 tcaaacttat atatattctt aaccatagat tttactttag atgtatggtt ttttgaagat
    26821 tgaaaatact gccttaccct attttttaga gatatggctt ttcccacata tattatatgg
    26881 tcgtatttgt ctttcattaa atatactccg ggctcagaag gtaatttttt taaatgctct
    26941 tgtatatcaa acatctcgcc ctccaaaaca ttaaatttta tactaaaatt ataccctaaa
    27001 atatgaattt taaaaagtta tacaatttcc agtgctttaa acaagttaaa gcccatattc
    27061 ccttctataa ttgatttgaa aacaatgtta accatgaaga gtaatatgga ctttactttg
    27121 tattactatt tacaacttat atttgactaa tttacttata acaaaacagt ttatcgtata
    27181 tctgctctaa aatttttcac aatttatata acttaagaaa aatatttctt taagaattga
    27241 cctgtataag aaccatcaac tttagatact tcttttggtg ttccagttgc aactatagtt
    27301 ccacccttat ctccgccttc tggtcctaag tctattacat aatcacaagt ctttataaca
    27361 tctaaattat gttctatgac tataattgta tttcctgtat ctgctagtct ttgtaatact
    27421 tggattagct tgtctacatc tgccatatga agtccagtag taggttcatc taatatatac
    27481 aaagtctttc ctgttggtct tttactaagt tcagcagcta attttatacg ttgtgcttct
    27541 cctcctgata attgagttga aggctgtcct aatttaatat atgaaagtcc tacatccatt
    27601 aaagtttcta attttctttt tatatttggt atattttcga aaaactcaac agcttcttcc
    27661 acattcatat ctaatatatc agatatagtt ttatctttgt attttacttg caatgtttct
    27721 ctattgtatc tttcaccttt acaaatttca caaggaacat atacatctgg taagaaatgc
    27781 atttctattt ttattattcc atcaccctta caagcttcac atctaccacc ttttacatta
    27841 aaactaaatc tacctttttt ataacctctt gctttagctt cattagtaga tgcaaaaata
    27901 tctcttataa aatcaaatac acctgtataa gtagcaggat ttgaacgagg tgttcttcca
    27961 ataggacttt ggtctatatt tataacttta tctatacttt caattccttt tatctcctta
    28021 tgcttaccag ctctttgctt aagtctattc actttacttg caacaccttt gtaaagtata
    28081 tcgtttatta atgtactttt tccagaacct gaaacacctg ttatgcatgt aaacttacct
    28141 aatgggaact ttacatcaat atttttcaga ttattttctg atgctttaac tatttctata
    28201 aaacttccat ttccttctct agtagtctca ggaagcaata tttgtttttt accacttaaa
    28261 tattgacccg ttattgagtt ctcattttca attatttcat ctaaagtacc ctgtgcaact
    28321 atttcaccac catgtatacc tgcacctgga ccaatgtcta taacataatc agcttctctc
    28381 attgtgtctt catcatgctc tacaacgatt aaggtatttc ctaaatctgt tagatgtcta
    28441 agagtagtta taagcctatc attatctctt tggtgaagcc ctatacttgg ctcatcaagt
    28501 acatataaaa ctccaaccaa tgcagaacct atttgagtag ctaatcttat acgttgcgct
    28561 tctcctcctg ataaagttcc agcttttctt gataaattta aataatcaag acctacatct
    28621 cttaagaaca tagctcttcc ttttatctct tttataatct catgagctat aaatctctgt
    28681 ttttctgtaa gatttatatt ttccataaag tcaatcaatt catttacaga taagtttgta
    28741 acttccatta tattttttcc atcaactaaa acagataaaa cttccttttt aagtctcatc
    28801 cctttacact ttggacatgg tctctctgcc atgtattctt caatcttatc tctcatgtaa
    28861 tctgaattag tttctctata tcttctctct aagtttacta ttacaccttc aaagtatgat
    28921 ttataatttc tagttcctcc aaattttgac tcataagtaa actctacaat tatattatct
    28981 ttaccataaa gtaattcctc cacaaaatca tctggtaaat ctttaaacgg tgttgtcaaa
    29041 gatacattaa aatgctctgc taaacttttt atcattttac tgtaataagt gtcatcatta
    29101 acacctgttg aaccccatgc tgccactgca ccttgtttta ttgataaatc tttgtttggt
    29161 actactaatt ctgggtcaac ttctttactt tccccaagac cattacaaac atcacaagca
    29221 ccaaagggag cattaaatga aaacattctt ggagataatt cttctatgcc aattccatgt
    29281 tctgggcatg caaatttagt tgaaaaaaga atttcttctc catctattat ttgggctatt
    29341 acaagaccat cagatagctt aactgcagtt tcaattgaat ctgctagtct tgattctatt
    29401 ccatctttaa ttactattct gtccactaca acttctatat tatgtttttg atttttattt
    29461 aagattatat catcactaac ttcatagttt tcaccattaa ctctaactct tacaaatcct
    29521 tcttttttga tattatcaaa gactttttta tgtgtacctt tttgtcctct aacaattggt
    29581 gaaagaattt gtaatttcgt tctatctgga aaatctagca ttttatcaac tatttcttga
    29641 atagtcatct gacttatttt ttcaccacat gtagggcaat acacatctcc tactcttgca
    29701 aataatagtc tcaaataatc atatatttct gttactgttc caaccgtaga acgtgggttt
    29761 tttgaggtag tcttctggtc aattgagata gctggtgata aaccctctat atattctaca
    29821 tttggctttt ccatctgacc taaaaattgt cttgcataag cagatagact ttctacatat
    29881 cttctctgtc cttctgcata tattgtatca aatgccaaag aagacttccc agaccctgac
    29941 aaacctgtaa aaacaataaa tttatctctt ggtaattcta aatctatatt ttttaaatta
    30001 tgttcttttg ctcctcttat tataatttta tcttccataa tgttgttttt acctttctat
    30061 ttttccaatt tttcttctaa ttgtttcact ttatctctaa gttctgctgc tctttcaaat
    30121 tgtaagtttt gagcagcttc catcatctct gattttaatt tatctatatt agctcttatt
    30181 tcgtctttat catctgtatc agatattcca tatacaactt cttcctctgc aaccttagta
    30241 gcttctatac tatctcttat atttttctgt atagttttag gaatgatatt gtgctcttca
    30301 ttatataaat tttgtatatc tcttcttctc tttgtttcat caatagcatt ttgcattgag
    30361 tcagttatcc tgtctgcata cataattact cttccatttt catttctagc tgcacgtcca
    30421 atagtctgta ttaaagctgt ttcggaacgt aagaaacctt ctttgtctgc atctaaaatt
    30481 gctataagtg aaacttctgg tatatccaat ccttctctaa gtaagtttat tcctacaagg
    30541 acatcaaact tccctaatct taaatctctt attatttctg ttctttctaa tgtaactata
    30601 tctgagtgta aatatttaac ttttatccct atttctttaa gatagttagt caagtcctca
    30661 ctcatttttt tagttaaagt ggttataagc actctttcat ttttttctat agttttagtt
    30721 atttctccta ccaaatcatc tatttgatta tttataggtc ttacttctac aattgggtct
    30781 aaaaggccag ttggtcttat tatctgttct gctattgtct ctgagtgttg aatttcataa
    30841 ggccctggtg tagcacttac aaacaacatt tgatttatat ttccttcaaa ttcttgaaaa
    30901 tttaatggtc tattgtcaag tgcagaaggt aatctaaaac cattctctat aagtgatgtt
    30961 tttctagacc tgtctcctgc atacatacct cttacttgtg gtatagtgac atgtgcctca
    31021 tctacaatta tcaaataatc atctgggaag aaatccatta aagtatatgg tctttctcct
    31081 tctgaccttc ctgtaatatg tctagaatag ttctctatcc cctgacaaaa tcctatttcc
    31141 tttaacattt ctatatcata cttagttctt tgttcaattc tttgtgcttc taatagccta
    31201 tcattttcct tgaaaaattt aatttgttct tgaagctcgt tttctatttc tacaattgcc
    31261 ttttcaattc tttctggcgt tgttacatag tgagatgctg ggaatataac aacatgattt
    31321 ctagtaccaa caatcttccc tgttacataa tcaatttctg ttattctatc tacctcatca
    31381 ccaaaaaatt ctattcttat tgctttttca tcattactag ctgggaatat ttctagtata
    31441 tctcctctaa ctctaaaggt acctcgtgta aagtttatgt catttctttc gtattgtatc
    31501 tctattaacc tttttattac atcatctctg tctctttgca tacctggtct tatagataac
    31561 ataagttctt tatagtcttt tgggtcacca agtccatata tacaagatac agatgatatt
    31621 attatagtat ctcttctctc taaaatagat gctgtagcag aatgtcgaag tttatctatt
    31681 tcatcattta tgcttgcatc tttttctata taagtgtcac tgtgagctac atatgcttct
    31741 ggttgataat aatcatagta acttacaaag tattcaactg cattgtctgg gaaaaattct
    31801 ttaaactcac tgtaaagttg tgctgctaga gttttattat gagccattat tagggtaggt
    31861 ttcttaactt gttgaatgat atttgccatt gtgaatgttt ttcctgaacc cgttacacct
    31921 aataatgtcg aaaatttctc atttctattt atagaatcca ctattgattt aatagcttct
    31981 ggttggtcac ctgtaggttt gaagtctgat tttattttaa aatccatagt atcactcctt
    32041 ttttcgaaca ttagttcctg acatgtatta ttatatcata cctttataaa attgtttaag
    32101 ttattttaaa tattttaccc atttaaattt tacttaaatc taaaacataa aaaaagactt
    32161 ctatctttgc aatagaagtc tataattcat ggaaaataaa tactttattc aacttacata
    32221 ataaaatcaa atgcttcttt ttcaactcca tctaatttta aattaaacat ttttaatata
    32281 gttgctgcat gtgctctcag gtctccttct tctataacca caccaccttt aatatttggt
    32341 ccaaaagcta taaatggagg tctattacct ttagtaggta aatgaccatg tgtcgcaaca
    32401 gcaaacttgt aatcttttat atctgaatct tttataatac ttccttcgaa atcatttcca
    32461 aatgaaattc cttcaagacc ttcaattacc atttcaaaat caccttcaag accaaggtta
    32521 gatgcttcct ctttggtaaa aatattctct atgaataatt cttctttaaa ttcttcaagc
    32581 agttctctta actctgttaa cctatctaag ttatttacta ttatttgagc tgatattcca
    32641 gaagaattta cataggcatc aaaatcctta acttctccat tttcaacttt tataaatcca
    32701 tttgatttaa gcaagacatt tggatttata actttatcaa ctctaagatg accatggtcc
    32761 ccaagtatta taaaatttgt atctttgtat gtacctgcat cctttgtagc ctgaataata
    32821 tctcctagcc atttatcatt catttttaat gctttatcta cttcgtcatt aaataagcca
    32881 taattgtgtc gtgtatggtc caatgtagct aaatgtatta gcattaaatc tggtttatat
    32941 tcctttatta tatctactga acagtttact ccaaataaat ccatgttggg ttctaatttc
    33001 caatcgatat aatgacaatg tctattgtaa ataccgtcca ttataacctt actggaactt
    33061 cgttcaaaag tttctcttgg gtcatcttct ctactaggag cccatatttc tgcaatatta
    33121 taatctatat ttggatttgc tcccattaca ggccataata cactagaggt agttagatta
    33181 ttttcttttg ctacatctat aatagttttt gcttttatgt cctttgagta ccagtaccag
    33241 tctttatttt ctttatttgg gtcaaatttt tcattatgat atataccatg ttttttagga
    33301 tatactcctg taaccatact agtatgacac ggatatgtca gagtgggata aacacaattt
    33361 atatttttta ctatggagca attttctagt atagatttaa agtttggtag attttttaca
    33421 aattccaagt ctttttcaaa taaagaatct actgataata ctataacctt tgtcatttac
    33481 attacctctt catttatata ttattgaata cttttaatca acttacaagt ctatataatt
    33541 atttatttgt tttatctatt actgcttgca ttgcagaagt tcctttgctt actacatctt
    33601 ttggatttgc atttaaatca gtaagcccct ttttaatctc atccattagc ttatcagttg
    33661 cttccttgta tccatttaac attggaagtg caacagcata ttgtaattgg tcagttgcaa
    33721 ccttatattg aggtttttct ttatataatg tttgtaactt ttctgagttt gttgcatctt
    33781 cagttgttgg taagtatcct gttgatgaac taaactgaac gattttatct gtttgtgtca
    33841 tccattctat aaattctgca cttgcttctt tttctttatc tgttccacct tcaagcataa
    33901 ctagattagc tccacctgta ggagttgcgt atttttgatt ttttggtaag aatgctgttg
    33961 atatttcaaa cttaccttct gtttgttgca taagtcctgc taaatctgct gttgatgtaa
    34021 atatcatacc tactttacca tttacaaaat cttgttttgc tacgtcccag gcattataat
    34081 cttctccagg aggcattttc attattccag ctttcatcat atcttgccag aacttaactg
    34141 gtgcttgtcc tgcctcatta ttaaatgcta cttttttacc attagttatt tctcctccac
    34201 tttgcattac catagcttca tagaaccaaa tatctattgg tgtttcaaaa ccataagtat
    34261 cttctgattt atttgtcatt ttactagcat aatccttaag ctcttcccat gattttggac
    34321 ctgaagggtc aagacctgct ttagttgcca tatctttatt tatgtataat attggtgtac
    34381 ttctattaaa tggcatacca acaaacttat cctcataata tgaatttgtt aaaagaccag
    34441 aaaagaatct ttcttgatat gttttatcaa aatatttact catatctact aaagcacctg
    34501 ctgatgcgaa tgtacctgtt tgagttattt ctaataatgt tacatctggt tggtctttag
    34561 atgttaaggc tgcctgtagt tttgttgcat tttcatagta atctttctgt tttaacatat
    34621 ttacttgtac atccttatgt tcactattaa attggtcaac cattttttga gcagtttctc
    34681 cattagttcc acctaaagga gcccagaaat ctatcttcgt aatattgcta tcagtctttt
    34741 ctcctttaga tgcatttgca tcattagaat ttgaacaccc aactaaagaa cataataata
    34801 atgttattgc taatcctgtt gctaattttc ttttaagctt cattttcatc ctccaaattt
    34861 agtctttaat tttttatttt attcctgaat acataaaagc ttcttttact tttttattcg
    34921 caaatatata aacaagaatt attggtaaaa ttagaattat atttcctgcc attattacat
    34981 tccatgcagt aattccttct gaagatttaa ggagcgcaac tcctattggt aaagttctta
    35041 ttgcatctgt atttgtcatt actaagggcc agaaataatt attccaatgg tatataaaat
    35101 taaataatat aaatgttatt actgctggtt ttgccatagg aaccattatt ctaaacatta
    35161 tttttaagtc agaagcatta tctagtttag ctgcttcaat aatttcatct gaaatctgca
    35221 taaaattctg tctaagcaga aatatcccaa atgaagatgt cataaatgga attataagtg
    35281 gaacataagt atttataaat ccaaacttac tcatcatcat atagactggt agaaaagtta
    35341 cttgtggtgg aatcataagt cctacaagca aaagcccaaa tagcattttt tcacctttaa
    35401 atttaatctt agaaaatgca tatgctgctg gaaccataac taaaaattgt acaattaaaa
    35461 cacttcctgt tacaaataca ctgtttagca aatatctaaa aaatggacct gaatgccatg
    35521 ctgtactata attttctaac actatatcat ttggtataat agtgggcggt aattgcatca
    35581 tttcttgtgg atttttaaag gatgtagata gcatccatat aaaagggaat ataaatattg
    35641 cagataaaag taatagaatt aatatttcta ttgattttga tgctattttt tttatatcaa
    35701 ctttgtcttt tttgataggt tgtataatat ccattgtctc ctccttttgg ataaacctta
    35761 taatatctac tttttcttta ttgataatga actttctttg ataagagtct aaagttagca
    35821 tatgtagcaa tcataactat agctaacaaa aacatactcg ctgcactcgc ttgccctatg
    35881 tcatagtata taaatccaga ctcatatata taataagata acatgttagt tgaacccact
    35941 ggcccacctt gtgtcattat acttatctca tcaaatactt gaaaagatgc agttgttgtt
    36001 gttatcataa gaaaaaatat tgttggtgat aatagtggca atgtcacttt aaagaaattc
    36061 gttattttgt ttgcattatc taatttagat gcttcatata tatagtttgg tatggattta
    36121 agacctgata acactattaa gatattatat ccaagagtct tccatattcc tattagaatt
    36181 agtgacatta aagctgaact ttcacttgaa agccactttg atgttggtaa atgtaatgtc
    36241 gatagtatcc agtttaaaaa gccatattgt ggctccataa tccaaagcca caatacagct
    36301 acagatacta gtgaaattac atgtggtgta aagattatac tttgcgttaa attgtgtatt
    36361 atagaatttt tatttagcca aacagcaagt actatggcta gtgatataga tatagtcacc
    36421 ataaaaaaag tataaattgc agtgtttgtt atagtttgta taaactctgg attctttaaa
    36481 agttctgtat aattgtttaa gcctacattt tcaaaggttg aactaagaag actaccagat
    36541 ttgaagctta gagatatcgt cttaattatt ggatagacta taaacaataa agttcctata
    36601 atagcaggtg ttatatataa atatgcttta ttaaattgct ttttcataaa tatctccttt
    36661 gattttagaa tacatacttt tacatacttt tacatcttct attctttctc catttttatc
    36721 aaaataatat aaatcttttt ctaatacact tattcttaaa gttcttccaa tatcaaatat
    36781 atcattttcc gttttgactc ttatctctct attaagaaca gataaacaat atatagtttc
    36841 atttcccaac acttctttag ttacaacttt tccaaataga ctaaatacat catcattttt
    36901 cccatctatg tttatactct ctagtgttgc tttttctggt ctgaatccaa atttaatatc
    36961 attttttata tctagtatgt tcatacaagg actccctata aattgagcta caaataaatt
    37021 tttaggattt tgatatatct tctttggaga acctatttgc attatctttc ctttgttcat
    37081 tataactatc ttatctgcca ttgacattgc ctcaacctgg tcatgagtta cataaacaaa
    37141 tgtagacttt agtttttgat gtaattgtat taattctgtt ctcatttgat ttcttaattt
    37201 agcatctaaa tttgacaatg gttcatccat taaaaatacc tttggatttt taaccatagc
    37261 tctagcaagt gccactcttt gtctttgacc tccagataag ttgcctggtt tcacatttaa
    37321 atattctctc aatcctacta catcaataac ttctgttata agtttttctc ttttttcttt
    37381 ttctactttt ttgtttttaa gaccaaattc tatgttttct ctaacagtca tatgtggata
    37441 taatgcataa ttttgaaata ccatagctat atccctttta ctagcatccg tttttgtaac
    37501 atcattttct tcaatcttca acctacctga agttatatct tcaagacctg caatcattct
    37561 taacgtagta gatttaccac atccacttgg gcctaccaat actgtaaaag aaccatcttc
    37621 tatagtaaga tttaaatttt ctataactgt atttttttta ttataagatt ttgttacatt
    37681 ttctagtctt atatctgcca tttttatcct cccaaaatat ttaatttaat tctacgattt
    37741 acttatagat taataatact cttaatttgt aaaggatata ttaagacaaa gtgaatatat
    37801 ggtaaactta ttaaaataaa ggttaaatta taaacatttt ttgttaaata tactgaagtt
    37861 aagatgatat acagcttatg ttagctataa ttttctatat ttttcatatt catggtttgc
    37921 tatcacaatg taattttaat ttatgaagag tcatataaaa ctatttattt tttatataat
    37981 ttggtttgaa agtaaaaaag acctctatgg gggatagagg tctttaagtg ggggagattg
    38041 agtaatttat ttttgctata ttaagtttta caattatatt atgtcccaaa tttacaaggt
    38101 ttaaacataa aaaataaaaa ttttttaatt tttataaatt tacc
//
LOCUS       DAYAOY010000033        36841 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYAOY010000033 DAYAOY010000000
VERSION     DAYAOY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.033"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            322..1482
                     /locus_tag="ACGI6B_001729"
     CDS             322..1482
                     /locus_tag="ACGI6B_001729"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFG9751175.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1484..2614
                     /gene="glgD"
                     /locus_tag="ACGI6B_001730"
     CDS             1484..2614
                     /gene="glgD"
                     /locus_tag="ACGI6B_001730"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFG9751176.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2643..4085
                     /gene="glgA"
                     /locus_tag="ACGI6B_001731"
     CDS             2643..4085
                     /gene="glgA"
                     /locus_tag="ACGI6B_001731"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFG9751177.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4099..6519
                     /locus_tag="ACGI6B_001732"
     CDS             4099..6519
                     /locus_tag="ACGI6B_001732"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HFG9751178.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6524..8389
                     /locus_tag="ACGI6B_001733"
     CDS             6524..8389
                     /locus_tag="ACGI6B_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFG9751179.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8634..9287
                     /locus_tag="ACGI6B_001734"
     CDS             8634..9287
                     /locus_tag="ACGI6B_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9751180.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9383..9982
                     /locus_tag="ACGI6B_001735"
     CDS             9383..9982
                     /locus_tag="ACGI6B_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9751181.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
     gene            10572..12047
                     /locus_tag="ACGI6B_001736"
     CDS             10572..12047
                     /locus_tag="ACGI6B_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9751182.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            12134..12547
                     /gene="speD"
                     /locus_tag="ACGI6B_001737"
     CDS             12134..12547
                     /gene="speD"
                     /locus_tag="ACGI6B_001737"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFG9751183.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            12577..13428
                     /gene="speE"
                     /locus_tag="ACGI6B_001738"
     CDS             12577..13428
                     /gene="speE"
                     /locus_tag="ACGI6B_001738"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFG9751184.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            13418..14296
                     /gene="speB"
                     /locus_tag="ACGI6B_001739"
     CDS             13418..14296
                     /gene="speB"
                     /locus_tag="ACGI6B_001739"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFG9751185.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(14476..14676)
                     /locus_tag="ACGI6B_001740"
     CDS             complement(14476..14676)
                     /locus_tag="ACGI6B_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9751186.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            15143..16633
                     /locus_tag="ACGI6B_001741"
     CDS             15143..16633
                     /locus_tag="ACGI6B_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9751187.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            16913..18409
                     /locus_tag="ACGI6B_001742"
     CDS             16913..18409
                     /locus_tag="ACGI6B_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9751188.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            18543..19469
                     /locus_tag="ACGI6B_001743"
     CDS             18543..19469
                     /locus_tag="ACGI6B_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9751189.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            19587..19778
                     /locus_tag="ACGI6B_001744"
     CDS             19587..19778
                     /locus_tag="ACGI6B_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751190.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            20001..20351
                     /locus_tag="ACGI6B_001745"
     CDS             20001..20351
                     /locus_tag="ACGI6B_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HFG9751191.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            20612..22117
                     /locus_tag="ACGI6B_001746"
     CDS             20612..22117
                     /locus_tag="ACGI6B_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFG9751192.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            22507..23604
                     /gene="dinB"
                     /locus_tag="ACGI6B_001747"
     CDS             22507..23604
                     /gene="dinB"
                     /locus_tag="ACGI6B_001747"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFG9751193.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            23867..25003
                     /locus_tag="ACGI6B_001748"
     CDS             23867..25003
                     /locus_tag="ACGI6B_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751194.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            24996..26543
                     /locus_tag="ACGI6B_001749"
     CDS             24996..26543
                     /locus_tag="ACGI6B_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751195.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            26878..27744
                     /locus_tag="ACGI6B_001750"
     CDS             26878..27744
                     /locus_tag="ACGI6B_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9751196.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            27885..29027
                     /locus_tag="ACGI6B_001751"
     CDS             27885..29027
                     /locus_tag="ACGI6B_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9751197.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            29429..30649
                     /locus_tag="ACGI6B_001752"
     CDS             29429..30649
                     /locus_tag="ACGI6B_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HFG9751198.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKIGKMLKH
                     LITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLLYSVFYF
                     PGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIIIFNLTLH
                     GIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIVISNVNG
                     FIEILNIGIKSYPV"
     gene            30678..31565
                     /locus_tag="ACGI6B_001753"
     CDS             30678..31565
                     /locus_tag="ACGI6B_001753"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HFG9751199.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            31599..32228
                     /locus_tag="ACGI6B_001754"
     CDS             31599..32228
                     /locus_tag="ACGI6B_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HFG9751200.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            32260..32697
                     /locus_tag="ACGI6B_001755"
     CDS             32260..32697
                     /locus_tag="ACGI6B_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HFG9751201.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            32947..34314
                     /locus_tag="ACGI6B_001756"
     CDS             32947..34314
                     /locus_tag="ACGI6B_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9751202.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            34514..35788
                     /locus_tag="ACGI6B_001757"
     CDS             34514..35788
                     /locus_tag="ACGI6B_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFG9751203.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            35906..36625
                     /locus_tag="ACGI6B_001758"
     CDS             35906..36625
                     /locus_tag="ACGI6B_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFG9751204.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            36646..>36841
                     /locus_tag="ACGI6B_001759"
     CDS             36646..>36841
                     /locus_tag="ACGI6B_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751205.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKY"
BASE COUNT        14505 a         3760 c         6535 g        12041 t
ORIGIN      
        1 aacaaattaa aaaataagca tgttcatatg acacaaagga tttataaaaa atgtttttat
       61 atttataaaa taaaatttgt attatttttt aaccgataat tgaatattaa taaatactat
      121 agtgcttatg gaaaataatg tatacgtcgg taagtccatt atggggtata cattatatca
      181 attgtatagt tgatattatt ttatttttat taaggtatta tttaagcaac atttatacac
      241 gacatttatt gactgataaa gtttataaaa gtaaaaatgc aattataaat attaattact
      301 agatgagggg agtgtcatta aatgaaaaaa gagatgttag ctatgatttt ggcaggaggg
      361 caaggttcaa gattaggtgt ttttacaaaa agaattgcca aaccagctgt atcatttgga
      421 ggtaagtata gaattataga ttttgtgtta agcaattgtt caaactctgg tattgatact
      481 gtaggtgttt taactcaata tagaccgttg atattaaatt cacatatagg catgggaagt
      541 cattgggatt tggacagaat aaatggtggt gtctatgttt tacaaccatt tatgaatgaa
      601 aaagaaggaa attggtataa tggtacagca catgcgatat atcaaaatat ggactttgta
      661 gatacctata atccagaata tgtattgata ttatctggtg accatatata caagatggat
      721 tatagcaaaa tgttaaaatt tcataaggaa aaaggctcta aagctacaat agcagtgatt
      781 gaagttcctt gggatgaagc ttcaagattt gggataatga atacaaatga agattcaagt
      841 atctatgaat ttgaagaaaa accaagtgaa cctaagagca atttggcatc tatgggagta
      901 tacatatttg attggaaaat gttgagaaat tattttaagg aagcagaaaa aaatccagaa
      961 ataaattatg atgattttgg aaaaaattta atacctaaaa tgttagaaga taatgttgga
     1021 atgtatgctt atccatttaa aggatactgg agagatgtag gaactataca aagtctatgg
     1081 gatgctaata tggatataat aaaatctcca gaaacacttg atttagcaga ccctaagtgg
     1141 aagatatata caaatactat ggcaatgcct ccacaatata taggtaaaaa tgcaaatgtt
     1201 catagatcaa tgatagctga tggttgtaga attttaggtg aagtaggtaa ctctgtatta
     1261 tcacatggag ttgttgttgg taaaggaagt aaagtaattg attcagtaat aatgccaaat
     1321 gttgtaatag gtgaaaatgt aactatagaa aaagctatga taggtgagtg tgcaactata
     1381 aatgataatg ttcagataaa gaatgtaaat aatgaaataa atgtagtttc tgagtatgaa
     1441 aatatcgaac ctagatgcgt attaatagaa ggaggtcttt aagatgagaa atgaatgttt
     1501 agggataata aatttaaaca aaaaaggaga cccagcaata aacaaactta actatggaag
     1561 accaatagca tctacaccaa tagctggaag atatagaata atagactttg ctttatctaa
     1621 tatgataaat tcaggaatca ctaaagttgg aatattcgca aaagaaaaat acagatcatt
     1681 aacagaccat ataggtagtg gtaaagattg ggatttaagt agaaaaaaag gtggcctatc
     1741 aatttttagt ccagaaaaca ctaagtatag aaatacttac tcacatagag aaggtgatat
     1801 atatactatc ttagctaatc tagattatat agaaaagagt gaagaagaat atattttaat
     1861 agctccaagc tatatgctat gcaatttaaa ttattctcaa gcattggaat accataaaaa
     1921 atctcataat gatataacta taatatataa aaatgtaaat aatgcaaata aagattttgc
     1981 tggtaactta actctaaatt tagatagtaa caatagggta ataaatgtag gaaataattt
     2041 aggtaagttc cctagagcaa acatatgtat ggagacatat attatgaagc gtgaagattt
     2101 tgttgaatgt atatataaca ttgtaaataa aggcaattat tgttatttag aagaatttat
     2161 tattgaagaa gcagaaaaca tgaaaatagg agcctatgaa tatacaggat atctaaaatg
     2221 tgtaaattct gttgaatcat attttgaaat gagcaaagat ttattaaatt tagaagttgc
     2281 agatgaatta ttatattctg aaagaaagat atttacaaaa gaaaagaatg aatcacctac
     2341 aatttataca gatagtgcta aagtagaaaa tagctttata gcatctggtt gtttaataga
     2401 aggtactgta aaagatagta tcatatttag aaaggtaaat gttgaaaaag gaacagttat
     2461 agagaactca atagtaatgc aaaattgtgt aataaagtca aatgcaaaac tatataatgc
     2521 aatattagat aaaaatacaa gtgtatcaaa tgggaaagaa ttaagaggag atgaaaaata
     2581 tcctcttgta gtggaaaaga atacaaatat ataaataaat tttttagaag ggggaatgac
     2641 taatgaaggt tttttatgta acagcagagt gttggccctt tgcaaaaact ggaggattag
     2701 gagatgtttc ttatgcattg cctaaagaat taaaaaaaga aggtgtagat gtaagggtaa
     2761 taatgcccaa atattcaact ataccaagtt atttaaaaga tcaactaaaa gaaatagctg
     2821 tgtttagtgt acgtgttgga tggagaaatc aatactgtgg tcttttagaa atggaattgg
     2881 atggtgtaaa attttacttt atagataatg aattttattt tagaagagaa gatgaaagaa
     2941 agagtatcta tggatatgga gatgatgcag aaagatatac tttctttaca gatgcagttt
     3001 tagaagctat aagtagaatt gatttttatc cagatgtaat acatataaat gattggcata
     3061 cagggatgtt accattaatt ctaaaagaaa gatatgcaac ccttgagggg tataaaaata
     3121 taaaaactat gtatactata cataatctcc aatatcaagg tgtatttgat aagcatgttt
     3181 tatatgatat attggactta ccacaaaaat actttgataa tggagatata gagtattatg
     3241 gctcaataaa ttttatgaaa gctggtataa attttgcaga taaaattata actgttagtc
     3301 ctacttatgc aaatgaaata caaacatctt tttatggcga acaattagat ggattactta
     3361 gaaaagagtc aggaaaatta aaaggtatat taaatggtat agattatgat ttaaatgacc
     3421 ctgcaaaaga taaagatata tttgttcatt atgatgtaga ctcaattgac aaaaaagtag
     3481 aaaataaatt gagattacaa gatatattgg gactaaaaaa agattcatct attccgttga
     3541 taggtatagt ttcgagatta gtttctcaaa aaggatttga tttaatagca tacatgatgc
     3601 cagaacttat gagagaagat ttacagattg tcgttttagg aactggagaa catcaatatc
     3661 aatcaatgtt caactattac gattctaatt tttcagataa agtatcagct agaattactt
     3721 ttaatgcatc attggctcaa cagatatatg ctgcaagtga tatgttctta atgccatcat
     3781 tatttgagcc ttgtggtata ggtcaaatgt tagctatgag atatggttct ttaccaatag
     3841 ttagagagac tggagggctt agagatacag taacacctta taataaattt actggagaag
     3901 gcaatggatt tagttttaaa aattacaatg ctcatgagat gtttttctgt ttgaaaaatg
     3961 caatcaaagt atttaaggac aaagaaaaat ggattaaatt agttgaaaat gcaatgaaaa
     4021 cagataatag ttggaaaaaa tcagctaagg agtatataga aacatataga gacatatgtg
     4081 attaaaggag tggttttttt ggtaacaata agcaaaaaga aattcaagca aaaatttgaa
     4141 gtaaaaatgt atagcttata tgcacaatct atcaaggaag ctactgatga acaattgtta
     4201 aatgtattat gtagtctatt gaaagatgag atagcaaaaa aatgggttgc tacaaagtta
     4261 gataagaaaa aagaagttta ttattttagt ttagaatttt taatagggag acagttaaaa
     4321 tctaatttat taaatttaaa tattgaagaa gaagttagag aaggtttatc tgaattagga
     4381 atcaacttgg atgatttaat tgaagctgaa gtagacccag ccttaggaaa tggtggactt
     4441 ggtagattag cagcttgctt tttagattct atggcatctt tgaacataag tggtcaagga
     4501 tatggaataa ggtataaata tggtctgttt gaacaaaaat ttgtaaatgg ttatcaggta
     4561 gaagtgcctg ataactggct tacagaagga cgatatgcat gggaaactgt aagaccaaat
     4621 gaagcaacaa tggtgaagtt tggtggtgag gtagaattaa tcaaggaagg tagtcattta
     4681 aaagtaattc ataaaaacta ccttcctgta atggcaatgc cttatgatat accaataatc
     4741 ggataccaaa accaatgtat aaatacttta agattgttta agagtgaaat accaaagaga
     4801 gattttggtg aacttacctc aaatgctcta aattattctg gtagctatga agaggcttta
     4861 aaacataagt attatactga ggaaatatca caggtattat atcctgatga ttcaaactat
     4921 gcaggtaagt tgttgaggtt gaaacaagaa tacttttttg taagtgcagg gatacaagat
     4981 ataataagaa aatataaaaa gaacaaatta aatattaata atttattcga taaggtagca
     5041 attcatataa atgatacaca tcctacctta tgcataccag aacttatgag agttttgctt
     5101 gatgaagaaa atttatcttg ggatgaagca tgggaaataa ctaaaaaaac agtgtcctat
     5161 actaatcata ctataatgtc agaagcaatg gaaaaatggc ctgtaagtat gatgaaagaa
     5221 ctattaccaa gaatttatat gataatagaa gaaataaata gaagatatgt agaggaatta
     5281 aataataaag gatatgacca agatagaata aaaagaatga gtataataga ctgtgacaat
     5341 atcaatatgg caaacttatg tattgtcaca agtcatagtg taaatggagt tgctaagctt
     5401 catactcata ttcttgaaac agaggtatta aaagatttct atcaagatga accaaataaa
     5461 tttaataata agactaatgg aattgcacat aggcgatggc ttataagttc aaatcctcaa
     5521 cttagcaatt taattacaga tttaattggt gattcatgga agaaagatac attacaatta
     5581 aaaaatattg aaaaatttaa aaatgattcc tctgttttac aaagacttga tgatataaaa
     5641 tacaataata aagcaaatct agcaaagttt ataaaagata aatacgattt aaatgtagac
     5701 ccaagttcga tatttgatgt tcaagttaag agattgcatg cgtataaaag acagttactg
     5761 aatatattta atgtacttca tatgtatcat gaattacttg ataatcctaa tttgaatctt
     5821 gacccaagaa cttttatatt tggcgcaaaa gcagcaccag gatattattt ggctaaatgt
     5881 ataattaaat ttataaattc tgttgcaagt actataaata atgatgttag agtaaaggac
     5941 aagttaaaag tagtattttt agagaattat ggagtatcat tagcagaaat aataatacca
     6001 gcagcaaatg ttagtgagca aatttcaact acaactaagg aagcatctgg aacaagtaat
     6061 atgaagttta tgatgaatgg tgctataact ttggctacac ttgacggtgc aaatgtagag
     6121 atatgtgaac aggttggtaa agaaaatatg tttttatttg gacttagtgc agaacaagta
     6181 ctaaattata ataaatatgg tggatattct tcacttgacc tatatcattc taatatggat
     6241 ataaaaaggg tagtagatga tttaataaat ggatttattc ctaatcttgg cgaagaagga
     6301 agaagtattt ataattcact cactacttac aatgatgagt attttgtatt gagagatttt
     6361 gaaaattatg gtcaagctca agcagatata aacaggttat atagagataa agaaaaatgg
     6421 aataaaatgt ctttagttaa tatagctaat tctggatttt tttcttctga tagaacaatt
     6481 tctgagtatg caaaagacat ttggtttaaa agggtgtgat taaatgcaaa gtgtaataga
     6541 atataactct tgggataaaa attttaaggc tccgtttgga gctttaaaat ttgatgagga
     6601 attgaccatt tgtgttaaag taaatgaggg atacaatatc aagtctattt ccctagaaat
     6661 aaatagagaa gaagaaatga gaacaataac tttaaatgaa gaattagata atgataaact
     6721 tggaaagtgc ttttgtggta agattgaaaa gtttgatggt acaggtgttt atttttatta
     6781 ctttaaagtt gatgttgaaa tggatggtca gataaagact ttattttatg gaaaaaacag
     6841 agataatgga tattcttgtg aatataatta tagtgatatt aataaatatc aaatcactgt
     6901 ttacaaagat tttaaagtac caacatggta caaagaagga atactttatc atatatttgt
     6961 agatagattc aataatggaa atcgtagtgg taaggttgat aatcctaaaa aaaatagttt
     7021 catatatgga aattgggaag atacacctat gtacataaag gatagtcaag gggatgttat
     7081 aagatgggat ttccatggtg gaaatttaag agggataatc aataaacttg gatatttaaa
     7141 aaaattaggt gtaagtatac tatatctaag ccctatattt gaagcctcta gtaatcataa
     7201 gtatgataca ggtgactata aaaaaattga cccaatgttt ggggatgaag atacatttaa
     7261 agaacttatt gataaagcaa aagaaaaagg tatatcaata gtattagatg gagtttttag
     7321 tcatactgga gcagatagta aatattttaa tatgtatgga aactataata gtttaggggc
     7381 atatcaatct aaagaatctc catattattc atggtatatg tttgaagaat ttcctcaaaa
     7441 atataaaagt tggtgggatg ttaagacatt gccaaatata aatgaattag aacatagcta
     7501 tatggattat ataatatatg acaatgatag cgttataaat aaatgggtaa atatgggtat
     7561 taagggctgg agactagatg ttgcagacga attaccaact aagtttataa gagagttaaa
     7621 aaaggaatta aaaaaggcag atgatgactc aattcttata ggtgaagtat gggaagatgc
     7681 atcaaataag attagctatg gtcagagaag aagttattta ttaggtgaag aattagattc
     7741 tgttatgggt tatcctttta gaaataacat gttttctttc ttaaaaggag aaattaattc
     7801 atatgaactt tgtaatagat atatgcagat taaagaaaat tatccaaaag aatcatttag
     7861 gtctaattta aatttaattg gaacacatga tgttacaaga gctaaaacgg aattaaatga
     7921 tgatgtagat ttagtgaagc ttgctgtagc tatacagatg acatttgaag gagtaccata
     7981 tatatattat ggagatgaag caggtctttg tggaggtgtt gacccagata atagaagaac
     8041 ttatccatgg aaaaatgaag acgaagatat gcttaatttt tatagagatg ttataagaat
     8101 tagaaataag aataaacttt taagttctgg aaatacagaa tttatatata ctaataatga
     8161 ttctgtattt tcttttatta gagttaatga aaataatgat agaatgttaa ttttagtaaa
     8221 tagaagtgaa aatgtggaaa gtatatcatt atgtatagag agtagtttta ttgaggaaat
     8281 acctataaag tatagtctta aaaatgcaaa tagtactata caaattgaaa ataatgagtt
     8341 gaaagtagat ttagactcta aatcatttag aatttttaga gtaaattaaa tatctttttt
     8401 tagaaaaata aatagaaaat aaggtgaatg tggaaagagt tttgttttat caaaactttt
     8461 tctacttttt ttgtataaat tatcataaga attttagtat ttcaaaatat tacaaagaag
     8521 tataaaaaat atcataattg tgatattata taaattagta agataaaact gaactctcta
     8581 ccaaagttgt agggagattt aacttttata atcttaatat ggaggaacgt cttatgaagg
     8641 tagatccact tacaactcaa gtatatgact atatatcaaa gaaaatccaa aatggagaat
     8701 atgaagctaa tcagagaatt actgaatctg agatttgtaa atctatcgga gttagtagaa
     8761 caccagcgag agaagcatta actaggttag caggtgaaaa tttactagaa aaaataccta
     8821 ataagggatt tgtagtaaaa gaatttcagg aaaaggagaa attagacacc tattccgtaa
     8881 ttggagtatt ggatgcgtta gccggttctt cagcattaca aaatttaact gaatcagacc
     8941 tagtaaaaat ggaagaatta acagaaatga tggcggtctc tataaagtat aaaaattaca
     9001 atagctattt aaaattaagt aatgaatttc acgatatata cataactaaa tcagataatc
     9061 aggtgttagt aaatctattg aactctttaa gatataattt tatgtcaaag tcatatacta
     9121 gtgataatga agatgagtta tataaaatgt tgacatacag caataatcaa catattgaag
     9181 tagtaaaatc tatgagggag aatgatttag aaaatgttga aagaattttg agagaacatt
     9241 ggaaaacgat acctatggaa gagatgaaaa aagagaagct tttataaagt ttctctttat
     9301 tttttatacc attttatcac tatttttttg aattattaag tagaaaataa taattaattt
     9361 aaacttgagg aggaaaacta atatgtatcc aaataaagaa attgcaatag aagagttaga
     9421 agtggctggc aaaatgaatc ccggtccatg gataaaacat tcttacaatg ttgctaatgt
     9481 agctaaattg attgcagagc aatgtgaaaa tttgaatagt gaaaaggcct ttgtatgtgg
     9541 attattacat gacattggaa gaagaactgg tatttcttct gtaaggcata ttattgatgg
     9601 atatgattat gttatttcta agggatggga tgaagtggcg agggtatgtt tgacacattc
     9661 ttttcctgtt aaggatatag aagctgatat tggaaataaa gatattagta aagaacagta
     9721 tgattttatc aatcactatt taaacaacct tgaatataac gattatgata agttgattat
     9781 tctttgtgat gcattagcag atgcaaatgg gttttgtatt ttagaaaaaa gatttgttga
     9841 tacaacacga agatatggaa tatatccatt ttcagttgaa cgatggaata agacttatga
     9901 gtataaagaa tatttcgaga aaataatcaa caagtctata tatacattgt tacccaatat
     9961 tgaaaaatct atatattatt aaatcctaag ttaataattt aatatccagt atcaccagca
    10021 gttggtcatt atgattgact gcttttatta tgcttatatt aaaaaaatac actatggttt
    10081 gtgtatatca aagttatatg gaatgaaaag agaatttatt ttatcaatta aaaaatttaa
    10141 taaattttat attaaaatta aaatacaaaa ataggaaaat tatttagtcc aactaaacca
    10201 agaattttga tatgtgaaat aagaataata taaaagttta gtaataaaat acttataaat
    10261 taaaaatagt aaactcaaaa gcttactaaa actcaaaaaa gtaaaaaaaa caaaataata
    10321 aaaaataaga aagtttataa atactaagtt ttaaaaaaat taatataaac aacaaaaaaa
    10381 taaaaagttt ataaatattg atatagctaa tgtatatgtt tttgagtaac ttcaaaaaaa
    10441 cacttgtaaa aatgctgaga tgttaatata ataaatatca tgagtttaat aaaagtaaaa
    10501 ataaaaaata gaaataataa ataataaata ttaaaaatat ttgaatatag agaggagatg
    10561 aatttgaaat catggatagc gagtttgcaa atgcagttga attaagctat gagtgtgctc
    10621 cattattaga gtcgttaaag gaatactcag agaaagatat agcttgtttt gatgtgcctg
    10681 gacatgtaaa aaatagaggt gtagctatat taaataaata tctaggtgaa agtctcatga
    10741 aaatggatat aaattcatca ccaactatgg ataatgtatc tgcgccaaat gggattataa
    10801 aaaatgcaca agatttgcta gcacaagcat atatggctga tgaggcattc tttataacga
    10861 atggcacaac tcaagcaata catgcaatga ttttgagtgt tataaaacca ggtgaaaaag
    10921 tattacttcc aagaaatata cataaatcag taataaatgc gttaatactt tgtggaggaa
    10981 taccaatatt tatacaacca gaatttgatg aaaaactagg tataagttta aatattactc
    11041 ttgaaaaagt tagaacagag atagaaaaag attgtaatat aaaagcatta tttttcctta
    11101 atccaactta ctatggagtt tgtgcagacc ttgagagtat tatagaacta tgccataaaa
    11161 ataatgtttt agtgttagtt gatgaagctc atggtgcaca ttttccattt catttagatt
    11221 tacctccttc tgcaataagt ttgggagctg atatggtagc tgtaagtata cataaaactg
    11281 gaggagcatt gacacaatct tcagcactct tattgaatag agataatgta agttttgaaa
    11341 aggttcttca atcaatcaat atgctacaat caacatcagc atcttatttg cttatggcaa
    11401 gtatagatgg tgctagagtt aatctagttg agaatggtga aaagcaactg tcaaaagcat
    11461 taaatctatc taggtatgca aaatctaaat taaacaaaat tgatggaata aaagttttat
    11521 ctacagaaat attgaaacaa aaaggtgtta aatttataga tgaaacaaaa ttgtgtataa
    11581 atgtaaaaga attaaatttg actggatttg aagtatacga tttattatat aaaaattttt
    11641 caattcaagt agaattggga gattcatata atatactggc acttgtgtcc attggaacaa
    11701 ataaatcaga tatagataga ttagtaaaag cactttctat aattgcaaag atttatagaa
    11761 aagaatcaac attaaatgaa tttaatatgg ttcaaataaa tccaattatt aaacttaatc
    11821 caagagaagc tttttatgca ccaaaggaaa gtgtagaaat caattcatgt atagatagaa
    11881 tttgtggaga atcaataatg gcatatccac caggtatacc gattattgct ccaggagaat
    11941 tgataacaga agaaattatg gagtatatta ttttcttaaa aaatagcaat gcatatctta
    12001 cagatgtaca agataaaaac ttagatagaa tattagtaat aaaataaatt actttatttt
    12061 gcaagggcaa gtatttgtta agtagagttt atcaaataga acttattaaa agtataaagg
    12121 agttggggtt gaaatgaaat ttgaaacact aggaagacat atactagtag aatactataa
    12181 ttgtgatgaa aacgtactta aagacccacg tcttatagaa aattttatga atgattcagc
    12241 attaaatgca aaagcgacaa ttgtggattc tgtattccac cattttaatc cttggggggt
    12301 ttcaggagct gttataattg ctgaatctca tttgacaata catacatggc ctgaatatgg
    12361 ctatgcagca gcagattttt ttacttgtgg tgatatagac ccatggaaaa gttttgaatt
    12421 attagagcaa cttttaaagt ctgaaagaag cgagtctaca gaaattccaa gaggcttaac
    12481 tacaaaaatt aaaaaatatt caaaaaaaga tttaggtaag attactcata aaccagaagc
    12541 agtataaaac atacaagaga aagtgaggat tattatatgg aactttggta tacagaagaa
    12601 tggacagaaa atgtaagatt ttctataaaa gtaaataaac atctatttga aggaaagtct
    12661 caatttcaaa ggatagatgt gtttgatagt gatgaatttg gaaaatttct aacaatagat
    12721 ggattaatga tggtaacata taaagatgaa tttatatacc atgagatgat aactcatgtt
    12781 cctatggcta caaatttaaa cataaagaaa gtattagtca taggtggagg tgatggaggt
    12841 acagtaagag aattaagccg ttatcctcaa atagaaaaga ttgacatggt tgaaatagat
    12901 aaaatggttg tagatgtatc taaggagtat atggatatat gctcatgtaa attagatgat
    12961 aaaagagtga gtttatattt tgaagatggt gttaattttg ttaagtgtgc acatgataaa
    13021 tcatatgacc tgataatagt agattcaact gacccaatag gaccaggtga agggcttttc
    13081 tcaactgatt tttataagga ttgttataga atactgactg atgatggaat tttagtaaat
    13141 caaagtgaaa gtccgtattt tgactttaat gctaaagaga tgaaaagagc taataaaaaa
    13201 ttaaaacaga tatttccaat atcagaagtt tatcaagcac atataccaac atatccatct
    13261 ggtcactggt tgtttggatt tgcttcaaaa aaacttaatc cagtaaaaaa tcaagataga
    13321 aatggatggg agaaattaag tttaaaaact aaatattata atagtgatat acatttaggt
    13381 tcgtttatgt tacctcaata cgtgaaggag atgctagatg aagaataatt tctatcatat
    13441 gaacactttt atgagtatgg acaaaaatta tgaagaatct aatcttatag tatttggtgt
    13501 tggatttgat ggaactactt ctaacagacc tggggctaga tttgcaagta gctctatgag
    13561 aaaagaattt tacggtcttg agacatatag tcctttttta gatttggatt tagaggatta
    13621 taatatatgt gattatggag atttagaaat tagtgttgga agtacagaac aagtcttaaa
    13681 agaaatctat caagagacat ataagattgt tagagattca aaggtaccct ttatgattgg
    13741 aggagagcat ttagttacat taccagcctt taaagcagta catgaaaagt acaatgatat
    13801 atatgtaatt cattttgatg cccatactga tttgagggaa gaatataata atagtaaaaa
    13861 ttctcatgca acagtaatta aaagaatatg ggatattgta ggtgataata aaatatttca
    13921 atttggtata aggtctggga caaaagaaga atttaaattt gctacagaag aaaaacacac
    13981 atacatggaa ataggaggaa tagatacgtt tgaaaatata gttaatatgc taaatggaaa
    14041 gaatatttat ctaactatag atttagatgt attggatgca tctgtgtttc caggaacagg
    14101 tacaccagaa cctggtggtg taaattatag agagtttcaa gagattttta agattataaa
    14161 aaactctaat ataaatatag ttggttgtga cattgtagag ttaagccctg attacgatac
    14221 aacaggtgta tcgacagtta tagcttgtaa aatcttaaga gagttatgct taataatatc
    14281 tgataaaatt aaatagagta ttttatataa atagtatata ttataatata agaagttatt
    14341 ttaaaaaagt tagtttaagt ttgacgcttt tctaaagtaa cttcttattt ttgtaattaa
    14401 aaataaatat aataaacaac aaaaaagagc cctcgatagc tccttttcta aaatatatat
    14461 atttaaatta aataattata ttctatttac gttttcagct tgaggtcctc tattaccttg
    14521 agttatatca aagctaacct tttcgccttc ttctaaagtt ttgtatccat cattttgtat
    14581 agctgagaaa tgtacgaaaa catcatcttc tccctctaca gatatgaacc caaatccttt
    14641 ttcattgtta aaccatttta ctattccgtt tttcattata agaaaacctc cgcattatta
    14701 aaaaatacaa actaaaaaaa taagtctgtt ctattaatat aacataaatt ttaaataaaa
    14761 gcaaataaaa tttttaaaaa aattaattac ttttagaatt taataaaaat tgttaaatta
    14821 tacagtgaga agtattgtaa aatttgacga aaatgtaaat gtagataata aatttaatat
    14881 caaaaaatgt aacaaaaagg tatgtaagta aaaagtaaaa ttaaaatata taataaaaaa
    14941 tattatatat tagattgaaa agtaaaaata aaaatagtga aatttcagaa atatcaataa
    15001 tattatataa taaaacaaaa aaacactata tatattcgat aaaataaaat ttaaacatat
    15061 aatattttat gtatatatta ataaaaggac atattgtgtt ataatggtat cgtataaaat
    15121 catttacggg ggtggtatta aaatgtttca atttgaaaca caaatacaca aattaagaca
    15181 tgatgtttta actgcaatag ctagacttgg aaaagaagat aaccttacca gagaagcaat
    15241 ggataatatt cagtatgaaa taataaaagg tgagaaacct acatacagat gttgtgttta
    15301 taaagaaaga gctataatat ctgagagagt aaatgtaagt atggggttac aaccaggtca
    15361 agataagata gatttagata caatggatat agaagaagat gaacaaatta tatatatttt
    15421 ggaagctgca tgtgacagtt gtccaataaa tgaatttaca gtaacagata tttgtagagg
    15481 atgtctagca catagatgta aagaagcttg taaatttgga gctataacac atgtcggagg
    15541 aatggcctat ataaatcatg aattatgtaa ggcttgtgga atgtgtaaaa aagcttgtca
    15601 atatgatgcc atctcagagg ttgttagacc atgtaagagt gtttgtccaa ctaatgcctt
    15661 aggatttgat agagaaaata tgaaagctat gatacatgag gataaatgtt taaactgtgg
    15721 agcatgtatg tcagcatgtc catttggagc aatatctgat aagagtttga tagctcctgt
    15781 agctagaaag ttagttcaaa aagagaaaat gtatgcggtg gtagctccag ctataacagg
    15841 tcaagttgaa gcaaatgtta catatggtca aataaaaaat gctataaaat cactaggatt
    15901 tgttgatgta ttagaagcag cttgtggagc agatgcagtt acagttcatg aaagcttaga
    15961 atttgctgaa agaatggaaa agggagaagc ttttatgaca aattcttgtt gtccaggatt
    16021 tgttggttat atagaaaaaa catacccaaa tcatgtaggg aaaatatctt ctacagtatc
    16081 tcctatgata gctagtggta gatttataaa aagtcatgaa ccagatgcta aagttgtatt
    16141 cataggacca tgtacagcca aaaagagtga ggcagctaag aaagaattaa aaggagctgt
    16201 tgattatgta cttacttttg aagaaataat ggcattattt gaggcatttg atatagactt
    16261 ggctaagtgt tcaaatgaag atatagatga tgcatcaatt tttggaagag gttttggagc
    16321 tgctggtggt cttacaaaag ctatagaaaa ttatcttgct gaaaaaggta ttggtgttaa
    16381 ttttgaacct gtaaagattt ctggttcaag agaaatcaag aaaactatga ctatggctaa
    16441 tttaggaaag ctagaaggta attttataga aggaatgatg tgtgaaggtg gttgtataaa
    16501 tggttctggt aaattcttat caggtgcaaa ggttaaagcc acttttgata gaaaaaattc
    16561 tcaatcaact aagaaaagtg tcttagcaaa tacaaagata aaagatttta gtaatataaa
    16621 tttagaaaaa taaagataat acaaagcaac aaaaatacat attgttaata gagttgttct
    16681 aagttgaggt ttaacttttg gacaactcta tttttattta tatatttaat tattgaacaa
    16741 ctaaaataaa aattatatac acttaaaata aaaattacat atataatatt ttgtatataa
    16801 cttaacaaag tatatgccta tgttataata atttatagaa ataaataaaa aatacaatat
    16861 acataatagt agaaatatag caaaaaggtg tgatttatag ggaggattac ttatgtttga
    16921 atttgaaacg caactaaaaa ggttaaaaca tgaagtttta acagcagtag caagactatc
    16981 tattgaagaa aatttaacaa aagaaaatat agaaaaaatt ccatatgaaa taattaaagg
    17041 agaaaaggcg agatatagat gttgtgttta taaagagaga gaagtagttc ttgaaagggc
    17101 gcaacttgca ttaagtttaa ctccaaatag taaatatgga gatataaatc cagattcagt
    17161 tagtttggac acagatgagc aaattatata tatagttgaa gcagcatgtg atagatgtcc
    17221 aataaatgaa tttacagtta cagagctttg tagagggtgt ttggctcata gatgtaagga
    17281 agcatgtaaa tttggggcaa tttcttatat aaatgggaga gcatatataa atcatgattt
    17341 atgtaaggct tgtggaatgt gcaaaagttc atgtcaatat gatgctatat cagaagttgt
    17401 aagaccttgt aaaagcgttt gtccaacagg agctttggat tttaatagaa acactatgaa
    17461 agcaatgata catgaagata attgtataaa ttgtggagca tgtatgtcag catgtccttt
    17521 tggagcaata tctgataaga gtttgatagc acctgttgca aaaaaacttg caaagaaaga
    17581 aaatatgtat gcaatagtag ctccagctat aacaggtcaa atagatagta atattacata
    17641 tggtcagtta aaaaatgcta taaaatcact gggatttgta gatatgatag aagcttcttg
    17701 tggagcagat gcagttacag ttcatgaaag caatgaactt attgaaagaa taaaatcagg
    17761 agataattac atgacaaatt catgttgtcc gggatttttt aagtatatag agaagatgtt
    17821 cccagatgaa aaagatagaa tatcatctac agtgtctcct atggtagcaa caggaagatt
    17881 agtaaaaaaa atagattctg atgctaaagt tgtatttata ggtccatgta cagctaaaaa
    17941 gagtgaggca acagagaaaa gtttaaaaga ttcagtagat tatgtactta cttttgaaga
    18001 actaatggca ttatttgaag catttaatat agatttatca aaatgttcaa atgaagatat
    18061 aaatgatggt tcgatttttg gaaggggatt tggagcatct ggtggacttg ccaaatcagt
    18121 acagaactat attgctgaaa aggggcttag ttttgatttt gaacctataa aagtatctgg
    18181 tccaagacaa attaaaaaga caatgacaat ggctaaattg ggaagattag aaggtaattt
    18241 tatagaagga atgatgtgtg aagaaggttg tattaatgga gccggtaagt ttttatcagg
    18301 tgtaaaagcc aaaaatactt ttgatagaaa aaatggacaa tctactaaaa agagtgtttt
    18361 atctaatgat aagattaaag aatttgaaga tgtaaattta gaaagataat tgttttggta
    18421 cagtcatttt tttattatgc cacaaatatg cctaaaattt gcccttttta tatgtgtatt
    18481 ttacagagtt aaggttgcta tactataaat atgtaataaa taaaatttat aggagctttt
    18541 ggatgaggaa aatagtatta aaagatatcc tgtttttagt tactacatta tctataatag
    18601 taatattatt gcttgtaaca aatataacta ctaatagtaa gataaaatca acaagtgaat
    18661 cttatttaaa aagtgacaaa aatagtgtga ggcaaaatgg tatccagagg agcacaaaga
    18721 gtagcacaaa aaatactgca tttaagacta ctatactaaa cactggaaag tctgattgca
    18781 tattggttga gataggtaac aaagtcataa tgattgatac aggagaagat aaaaatggaa
    18841 aacaaatagt ggataggtta aaagaaaaag gaataaatac acttgactat ttgattttaa
    18901 ctcatttaga taaggaccat ataggtggag tagatagtgt gttatctagt gttaaagtaa
    18961 agaatattat acaagcaaat tataagaaag atagtaagca atatgatgaa tatattgact
    19021 cacttaaaaa agctgatata gaaccagtgc tactaaaaga taatatgaat attgtaatca
    19081 atggtgcaga aataaacata catccggctt caaaaagcaa gtacgagagt tcaaatgatt
    19141 attcaataat cactaatata agctatggag cacataagtt tttatttgca ggagatgcag
    19201 aggaagaaag attgtcagag tttataaatg gaaatacttt aaaatatgat tttgttaaaa
    19261 tgcctcatca tggaagatat gacaaattaa ctgaaacatt tttagagtct atttcacctc
    19321 aatatgctgt tattacatgt tcagagaaaa aagaaccaga ggaaggtgtt ttaaaaatat
    19381 tggaaagatt gaatataaaa acatttttga cttctaatgg agaggtagtt atcaatagtg
    19441 atggtaagac attgagtgtg aatcaataat ttttttaaaa atatctaaaa aaatttaaaa
    19501 aaatcattag caaatattac cccttaaaca tagtatattt taggagctga aactccacat
    19561 acaaaatgac taggaggtaa catattatgt tggatagatt ttttggtgaa ggcggatttg
    19621 gtggatgtgc ttggtggata ataatattat tcttcttatt cttagcattc caagattgtt
    19681 gggctgaaat ggacttatgt gcatggatac ctttcttaat attattatta atactttgtg
    19741 aatgtggtga cgtttttgat ggaggatgtg gatgctaaaa aaatcaattt agatgattta
    19801 ataaagctta tggtgggtat aaataaaata ccaaaccaat cacaatctag tgtttgaagg
    19861 tagtcggaaa tcccccgcta ccttcttttt tttattgtta aaatttattt ttgaattatt
    19921 tcatttacat ataataattt ttacgataaa gtctgatata attaaaaaga aaataaaatt
    19981 tatttatagg agatgtaaaa atggctatag tggataagat tttcaatgag ataaaactta
    20041 tggttgcaag acttttattg ggaaaaaaat acagtgaata tgaagaaaaa gatttagtgt
    20101 ataatacaga agaagaagtt atacttataa ctttaaaaag attagttttt cagggaaatg
    20161 taaacgaagc agaagaaatt ttatttgata aagctaagag tgtaaatagt gaaaatatgc
    20221 agtatatagc aatagaattt tatactatgc ttatggaaaa gactgatgaa gaattagaag
    20281 ctatgaattt ttctaaacaa gaagtttatc aaggtataga agatattaga aaagtattga
    20341 atttagaata aaagatatat gaaaatattg aaataaatta atgctaaggt gagaatatta
    20401 tacttatata taaaaatata gtattcttat tttattagtt atataggtat atcttattta
    20461 ccataaaact atcttgaaaa aaattaatct taattgtata atattacttt gagtgaaaaa
    20521 gtattaacat ttttttataa gatgttttac taaaaaaata tatataaata tactgttatc
    20581 aacaagaaat ttaatttagt gaggtgttaa gatgaaagta ggatttgacc acaaaaaata
    20641 tttagaagag cagtctaaat atatcttaga aagagtaaat aattttgaca aactttattt
    20701 agagtttgga ggaaaattga tgggagacct tcatgcaaaa agagtacttc caggatttga
    20761 tgaaaatgca aagataaaag tattacaaca tatgaaagaa aaagttgaag ttgtaatttg
    20821 tgtctatgca ggagatatag aaagaaacaa aataagagga gactttggta tcacttatga
    20881 tatggaagtt ttaagattaa tagatgattt aagaggttat gaactagatg taaatagtgt
    20941 agtaattact agatttgaag gacaacctgc tacaactgta tttataaata aattagaacg
    21001 tagaggaatt aaagtttata agcattatcc aacaaaggga tatccgtcag atgttgagac
    21061 tatagttagt gatgagggat atggagcaaa tgaatatata gaaactacaa agccaatagt
    21121 agtagtcaca gcaccaggac ctaatagtgg aaaacttggt acttgtctaa gtcagttgta
    21181 tcatgaaaat aaaagaggta atgtagttgg atattctaag tttgagacat tcccagtttg
    21241 gaatgtacct gtaaaacatc ctttgaatat agcttatgaa gcagctacag ttgatttaaa
    21301 agatgtgaat atgatagatt cattccacct tgaaacgtat ggtgaaatgt cagtaaacta
    21361 caatagagat ttggaattat ttccagtgct taaaaaaata atagaaaaga taactggaaa
    21421 agaatcaatt ttccaatctc caactgatat gggtgttaat agagttggct ttggaataat
    21481 agatgatgaa gtaataagaa aagcttctat agaagaaata ataagaagat actttaaaac
    21541 tgcctgtgag tataaaaagg gacaagttga taaaggtgct tatgatagaa tgaaaatgat
    21601 tatggaagag cttaacttaa agccagaaga tagaaatgtt gtattacctg ctagaaacta
    21661 cagtaataaa ttgaaagaaa gtgccgacaa gaatgatact tgtcctgtag ttgcattaga
    21721 attagaagat ggtacaatat taactggtaa gggttctgat ttaatgaatg gaactgcagc
    21781 agtagtatta aatgccatta aacatttagc caatatatct gatgatatgc acttaatatc
    21841 acctgtaata ttagaaccta tcataaactt aaaaacaaag actttagcta gtagaaatgt
    21901 ttcacttagt tgccaagagg tgttaactgc acttagtatt tgtgctgtaa ctaatccaac
    21961 tgcacaattt gctatggaaa agttagcatt attaaaaggg tgtcaagctc attctactac
    22021 tatattaaat agagatgatg aacaaacatt tagaaaactt ggtatagatg taacttgtga
    22081 cccagaatat ccttcacaaa atctttacta tagttaaaat tatataaaaa aatatgggct
    22141 gttttaaagt aatagtttga gcagtccatt acaattataa taaaatcagt aggctgtctt
    22201 gtataagaca gtcttttgtt tttaaaacat cccataaatt aaatatatat actgttatcc
    22261 ataaggaatt ttaaatttag ttccttggaa tacctattgt aaagctttta tatagttttt
    22321 gcaagtgttt ttaaggattt tttataaatt atattgaagt taatggatta tagatggttt
    22381 tatttatgga aaatatgaat atataaagaa aataatgaaa aaataccaaa aagaaacata
    22441 agtttggtat aattaaaaag taatataaat aaaattaatc tatttatact ttaaaaggtg
    22501 ataataatga aacattatag aaaaataatt catatagata tggacgcttt ttatgcatct
    22561 atagaacaaa gagataataa aaaattaaaa ggaagacctg ttattgtcgg tggaaatcca
    22621 caaagccgag gtgtggttgc aacctgttct tatgaagcta gaaaatttgg tatccattca
    22681 gctatgccat cagctgtagc ttacaatagg tgtccatatg cagtttttgt aagacctaga
    22741 tttgatgttt ataagagtgt gtcagagcaa ataagagata tattttatag atacacagat
    22801 ttagttgaac cattatcttt agatgaagct tacttagatg taacaaaaaa taaaaaaaat
    22861 attgattcat caattgagat agcaaagcaa ataaaaaaag atatatttag agaagttggt
    22921 cttacttctt cagctggtgt gtcatacaat aagtttcttg ctaagattgc atcagactta
    22981 agaaagccaa atgggttaac tgtaataacc gaagaaaatg cccaggactt tttagataaa
    23041 ttacctgtga ataaattttt tggtgttgga aaagttactt caaatactct taaaaattta
    23101 ggtataaaaa ctggatatga tttaaggtgt ttaaatttat ttgaacttga aaatatattc
    23161 aaaaaaagag gttatgaatt atataaattt gctagaggaa tagacgatag accagtggaa
    23221 ccaaataggg ttagaaaatc tgttggggct gagactacat taagtcataa tttggatatt
    23281 gatgaagaag aaacacgcaa cattcttgat gaactttgtg aagaagtatg tcatagatta
    23341 aaaaactctg agaagtttgg aaaaacatta acattaaaaa ttaaatatga ggactttaca
    23401 aagataacta gaagccttag tttagaacac tatattgacg aatataatga tataagaagt
    23461 ggtgtagata atttgttgag aaatgttgag gttaatggaa aacaaattag gctattggga
    23521 gttacaatat caaacctaag tgataaaaaa gaaacttata aagatattac attatttgaa
    23581 tacatggata gtatccaaat gtgagttttg tttaaatgaa ataacactag ctttaatact
    23641 atataattga ggctagtgtt attttatgaa tatttttata ttttttgttt tttgacattg
    23701 tatattatat actatatata aatactttat atttgtagca atattcagac aaatagtgac
    23761 ctaaaaatcg tactttgttt acataaataa aatagtatga tataattata aaaacaactt
    23821 ataatattgg agaaggttgc ttgttcgaaa aaggggatga taaattatga tagagataag
    23881 aaatgtaaca aaaaaaatag gaaataatgt tatattagat gatatttctc tagttgtaga
    23941 aacaggtaca ttggtagttt tgattggttc aagtggttgt ggaaagacta ctactttgaa
    24001 gcttataaac aaattaataa aacctacatc tggtgagata tatattaatg gcaaaccaat
    24061 ttctcaggaa aatgaaatag agcttagaag aaaaatagga tatgttatac agaacacagg
    24121 cctattccct catcttacaa taaaagaaaa tatagagcta attccacgcc taaaaaagga
    24181 aaaatccgtt gaagaaattg aaaaaagaac gttgcaacta cttgagatgg taggacttga
    24241 tagtgatgaa tttttgaata agtatccttc agaacttagt ggtggacagc aacaaagaat
    24301 cggtgttgca agagccattg ctactgatgc tgaaataata ttgatggatg agccttttag
    24361 tgctttagac ccaataacga gaacatcttt acaagaacaa ttattttctc ttcaagatga
    24421 attgaaaaaa actataatat ttgtaacgca tgatatggat gaggctttaa agatagctga
    24481 taaaatttgt attatgaagg atggaagaat agcacaatat gatactcccg aaaatatact
    24541 tagaaagcct gcaaatgact ttgtaaaaga ttttatcgga gaagatagag tttgggataa
    24601 tccagaatat ataaaagcaa gagatattat gataaaaaat cctattgctg ttaattctac
    24661 tagaactgta actcaaggaa tagaaattat gagaacgagt aaggttgata gcttactaat
    24721 tatagataga gcaaaaacat taaaaggtat tgttactgta aaagatatga aagatattga
    24781 tgataagtca atattacttg ctgatattat gtcaagtgag cctttgcatg taaatgaagg
    24841 ggataattta gtagaaatac taaatgtaat gaatcgtaat tcggttggat atattccggt
    24901 tatttctgat gaaaataaac tggtagggct tattactaga agtagtttac tttctgtttt
    24961 aagtgaacaa ttcttagaaa tggaggtgag tgttcttggc taattttttt aactttatac
    25021 tattacaaaa agataagatt atagagcttt taatacaaca tatgagtcta actgttacat
    25081 ctattttaat agctataata gttggtgttc ctcttggaat aattatttca agaatctcat
    25141 ctcttagaaa gtttgtactt ggatttgtaa acttagtcca agcagttccg tctatggcac
    25201 ttttgggtct tcttgtacct atacttggta taggaagtaa accagccata tttatggtag
    25261 tggtttattc attactacca attgtaaaaa atacgtatat aggtataaca agtattgacc
    25321 cagtagtatt agaatcagct aaaggtattg gtcttacaag aaatcagaca ttatttaaaa
    25381 tacaattccc tttagcactt cctataataa tgggtggagt tagaatatct gcagttacag
    25441 ctgttggttt gatgactctt gcagcattta taggtgctgg tggtttagga tatttagtat
    25501 tttcaggggt tcaaactgta aataataata tgattttagc aggggcaatc ccatcatgta
    25561 taattgcact gttagttgat tatttattta gtaaaattga agttgcagtt actccaaaag
    25621 gattaaatcc aaaagcacct aagaagaatt atatagcact taaagtaata tctgtagtgg
    25681 ttgttgtttc aatgttgttc gttgttttta gttctagttt ttcaagtaaa aaagatacaa
    25741 taactattgg ttctaaagat tatacagaac aacttatact tggtaatgtt tatgctgaat
    25801 tagtagaaaa aaatacgaat cttaaagtta agaagaattt aaatctaggt ggttcatctg
    25861 ttgcgtttaa tgcaattaaa tctggtgagt tagacatgta tgttgactat acaggtacac
    25921 ttttggtaaa tgtaatgaaa catgctccga ttaaagatgc tgatgaagca tataatgttg
    25981 taaaagatac tatggaaaaa gaaaatcagt taactttact tgaccctctt ggatttaata
    26041 atacttacac attagctatg atgcctgaaa ctgctgaaaa gtatggaata aatactattt
    26101 ctgatttgac taaatatggt aaagaattta cattctcacc aactttggag tttgaaaata
    26161 gagaagatgg tcttgtaggg ctttctagag attatggttt aaaattcaaa gatgtaaaag
    26221 caatgaatgg aagtttaaga tatacagctt tagataataa tgaatctcag gttatagatg
    26281 catttttaac tgatggtctt attaaaaagt ttaatcttaa gatattagag gacgataaaa
    26341 atttctttgt accatattat gcagcaccat tagtaagaga agatactttg aagaaatatc
    26401 ctgagttaga aaaagttatg aatatgcttt caggaaaaat aaatgaagaa actatgagag
    26461 aacttaacta tcaagtggat gaacttggta aatcaccaga agaagttgct cattcattct
    26521 tagttaaaga aggcttagta taaaaaatta atagtttata tcaaaaaatt taagagagtg
    26581 tctcaaaatg aactttttag ttcatgaggc actctttttt atatgaaatc aaatatattt
    26641 tatcatttca acaataaaaa agaagattat ctttattttg agacagtctc ttatttttat
    26701 gtataccaaa accataccta cgatttttat ttataattaa gtttcaaata gaatgaacaa
    26761 gtacaaccaa ttttgaatat aaatatagta aatgtgctta tatacgattt tattgtctgg
    26821 ttgattgata ttgatagata gctttcttta ttgaatcgaa gggaggggga atttgttatg
    26881 gataactata agaaagtgaa gcaagtattg tggataattt tatttgctaa ttttgcagta
    26941 gcattattaa aaatcattat tggtaatcaa ataaaaagct atagtatgac agcagacgga
    27001 tttcattctt tgtcagatgg agcatctaat atagtggggc ttataggtat tttctttgca
    27061 tctaaaccaa aagataaaaa tcatccttat ggacataaaa agtttgaaat tataaccagt
    27121 ttatttataa gtggtatgtt gtttgttata gcaattaaga taattcttag tgcagtgtta
    27181 agaatagcta atccagttgt acctgctata actatagaaa gtttaattgc tttaattatt
    27241 acacttttta taaatatttt tgtatgcatg tatgaatata gaattgggac taaactaaat
    27301 agttatgttt tgatttcgga ttcattgcat acaagaagtg atatatttgt gtctctaggt
    27361 gtattggtta ctttagttgg tgttaagtta ggatttccag taataataga atctatagtt
    27421 cctattatta tttctgcatt cataatttat tcggcttatg gaatatttag gccatctatt
    27481 ggaattttgg tcgatagagt ggctgtagat gaggattata taaaagaaat tgtgtttgaa
    27541 tttaatgagg taagggatgt tcacaatatc agaagtagag gtagcaaaag ttctatttat
    27601 attgacatgc atgtaatggt agacccattt ataagcgtag agcaatctca tgatttaaca
    27661 cataaaattg agaaacaaat acaagaagaa attaatgaaa atgctcaagt aatagtccat
    27721 attgaacctt tttatagttt ttaacttagc tataaaatac aagttataaa aaaatataat
    27781 ttataaaaaa aatattatac cataaataat ttataaaaat tatttatggt ataatatttt
    27841 ctataaagaa attaagtctt aatatatcta aggaggaatt tacaatgaga tacgttgaat
    27901 atggaaagac agggaaaatg gtttcagtag ttggctatgg aggattaagg tttgatttag
    27961 aaaaaagtaa tcaagaaaat gctgatttag tcaaatatgc gtatgaaaaa ggtattaatt
    28021 actttgatac agcaccaggg tattgtgatg acagaagtga agatatattt ggactagcct
    28081 ttagacaaat gataaaggaa ggtaagaccg atttttatgt atcaactaaa gaaaagccaa
    28141 aagcatgtga tacatcagaa aaagctatag aggctgtaaa aaaatcaata gaacgattag
    28201 gcgtatctaa aataaatttt tatcatgttt ggaatattag aaagatggaa cattacgaac
    28261 tagcaatgag acctggaggt caatatgaag gattattgaa atgtaaagaa gaaggattga
    28321 tagaccatat agtattttca tctcatcaac caggagatga ggttatagat attctaagtg
    28381 aaaataagtt tgaaggtgta actatgggaa taaatatatt aaactttcca tataggctaa
    28441 aaggtgccaa atatgctgtt gataatggat atggtgttgt ggcaatgaat ccattaagtg
    28501 gtggaactat tccaaagtat aataaagaac tttcttttct atcaaaagat ggcgaaagta
    28561 ctatagaatc agctttaaga ttcaacatag gaatttcaca aataagtata tctttgatag
    28621 ggtttaataa aaaacaagat atagatgatg catgtaaaat tgcagacgaa aatagaattt
    28681 attcagatga ggatatacat gatattgaga ctagattaaa taagaatatg aacgaaatat
    28741 gtacaggttg tggttattgt aaagtatgtc caaaaggtat taatacccca gcctatatgc
    28801 ttttttataa tgaaaagcag atgtttaaaa agagtgatga agaaatgaca aagttagttt
    28861 atggcttagg acattggaat tacacaatga atagtaaggc aaaagctaaa gaatgtatat
    28921 cttgtggaaa atgtgaagtt gaatgtactc agcatttacc tataatagat agattaaaag
    28981 aaattaaaaa atgggaagaa gatggagcta atagtgttaa agtctaatta attttatact
    29041 atatttgaaa tttgtatagt atttagtaat gttttaaaat atagttttaa tatttaagga
    29101 gacagattat aattttgtct ccttaaatta tgctttaatt tgttatagtt taattccata
    29161 agtaacattg tatattttta tataaagttt ttcaaatatt ttacattaat attgcttaca
    29221 aattctcatt ttatacaaca ctaatctatg ttatataaac aatacttgac tgattataac
    29281 atcaataaag taaatattgt ggttgtaaat aaagagttat tcaattacaa taaaacaagt
    29341 aagaacatta tgaagatggc tattaaatat aaggaataaa ttgtaggtgt cagcaaatcc
    29401 tatataatga atggatggtg atatattgat gagtaaaata ttaaaatata aaaacgagat
    29461 gtttttgttt atattagttg taataactta cttaataata actaagatat ttttttctaa
    29521 gactactata ttttatgatt taaacaatac ttatgatgta ttgttagata cagatacagg
    29581 agttttattc aatttaaatg tatttgctat aagtcaagac aatagtaagc atatattgtt
    29641 ttcagcaatt atatcaattt ttgcatatcc aatataccta ttttgtactt caatagcaaa
    29701 tcctgggaca acagatttta atagtgccta tgggtttgga ctaatttgtc tacaaataat
    29761 aactagtgct atgtcaataa ctttagtctt taatcatatt aaaaagataa aaatgcagag
    29821 attaacttta attttactta ctatgataat gatattttct tttccacagt tatttatgac
    29881 tttaaatgta gagagattta tatatagcca attttcatta atatttttca tagttattgc
    29941 aaataagatg aaaggaaaaa attcttattt ggtagaatta gcagctattc ctctatttgg
    30001 aataactatt agtaatatat atttgtattt ttttaatatg atatttgagt ttaaattaaa
    30061 aataggaaaa atgttaaaac atttaattac atttatccta atggcatata tttgtgtagt
    30121 ttcaacgaag tcttatgaga gttttatgaa ccttggaaat gtaatacagt atgatacgaa
    30181 atttataagt ggagaaccaa ttttggaaaa gattgcaatg attatagaga gattattgta
    30241 ttcggttttt tattttcctg gagcaagtat taaaaaaggg ttatttttgc aaaatggaga
    30301 agttgctaca ataccagtaa ttcttacatt attagcttta tgtttttgtg ttttatcagt
    30361 tattgaaaat tcagaaaaaa gagttcctaa gctatgtatg ggaataatta tattcaatct
    30421 aactttacat ggtatagtag gatataatct agtcaattca agtattatgg ctataaattt
    30481 tagttttgca gttataattt tattggctta ctttacgaaa gcccttagaa aaaatgaaaa
    30541 gaatatgtat aatatatttc tatcgctgtt attggttact atagtaatca gtaatgtcaa
    30601 tggatttata gaaattttaa acatagggat aaaatcatat cctgtttaaa aaataatatt
    30661 gaaagggtag taagtttatg aaacagacca atacaagtat aaggcttctt actaaaattt
    30721 cagattatat aaagttaatg agaataaagc aatatataaa gaatttcttt gtattttcag
    30781 ctataatttt ttcaaacaac atactaaata taaatttatt tttaaatacg tttattgcgt
    30841 ttgtgtgttt ttgcctaatg tcatcatcag tatatgcact gaatgatgct atagacatgg
    30901 ataaagataa aaaacatcct aaaaaatgta atagaccagt ggcaagtgga agaataagca
    30961 aaaaaagtgc taatatacta tttgtagtat tagcgttaat ttcagtctgt ttatcaacag
    31021 ttgttagttt taatctttct ataattctca gtctctattt gataaacaat atattatatt
    31081 ctcttaaact aaagaatata atactattag atgtgttttc aatatctctt ggttttatac
    31141 ttagggttta tgcaggatgt gtagccataa gtgttagttt atcaaattgg ataattttat
    31201 gtacattttt cttatctctg tacttagctc ttggaaaacg taaaaaagaa atagaaactc
    31261 taagagatga tgcagttgaa catagaaaaa tattggaaga ttatgacata gaaaatctaa
    31321 atcaaatgat gatagttata ttaagttcta caattgtatg ttatgcattg tattctacaa
    31381 gtaatccaga aaagccacat atgatattta caacaatatt tgtagtttat ggtgtactta
    31441 gatataatta tataattaac actacaaatg aaaataatcc tactgatatt gtcctaaaag
    31501 ataatgcttt aaaaataaac gtaatcttat ggataattac atgtttaata atattgattt
    31561 tttagtgata tttttagtga taggatgggg aaatacttat gaaaaaaagt gtattattac
    31621 ttgatgtaga ttatactgtt ataaatactg atagcatgat agattttttt atttactctt
    31681 taaaaaataa aacctttaaa actattataa agttgccata cattattttt attctattta
    31741 tgtatttaat tagaatgata cccttgaaga aggcaaaaga ggcaatcttt tatccaatag
    31801 tagattttag tgaagaagat ttaaaaaagt tttttgatga ttgcataatg aaaaaaatta
    31861 acgaatctat gaaaaaggtt atttacaaaa ataaagaaga agataatgtt ataattatga
    31921 taactgcatc tccatatgca tatatgaaat attttaagta ttatggtctt gcagacgagg
    31981 ttataggcac ggagtttttt tatgaaaatt caagatataa aaatcaattc attggagaaa
    32041 actgtaaggg aattgaaaaa gtaaaaagaa taaaggctgt attaggtaaa cttgggatag
    32101 agattgatta tgaaaattca tatgcatatt cagactctaa gagtgattta ccaatgcttt
    32161 cattgactaa aaatgcattt ttagttagta aaaaggatgg tgaggttata gagcaaataa
    32221 attcataact taatgtcact tagaatagga gggaatagtt tgggatattt acacatatta
    32281 attttatcta tggttcctgt aacagaattg aggggagcca ttcctatagg tattgcaatg
    32341 ggcttaaatc ctatatgggt ttatgtattt agtgtaatag gttctacatt ggtatcgatt
    32401 cccttgatat tgacattcag acatgtatta caatttttaa gagggaagaa actatttaaa
    32461 gggattgctg atgtaattga tagaaaaata aactcaagaa tgaagaaatt aaaaagtgtt
    32521 tcgataattg gtataatact atttgtaggt ataccactgc caactacagg aacatggact
    32581 gcatctgcta tagcttccat attaaaaatg agaataaaag atgcttttat gggagtattt
    32641 cttggcaatt tgttatcagg ggttatagtt tcagctcttt ctttacatat aatataaaac
    32701 tatctaagta atatacttgg atagtttttt tatgagtata tgtgaaaaat ttttaataat
    32761 aaataaaaaa tgagataaag tatttaactt cagttagttc taaaatgttt actatggcaa
    32821 aagaaatata gtatagtatt acttgtaggg aaagtactct aaatttgtct gctaataaaa
    32881 tgaaattttt atatagaata aaattttagt gatacatttt aattattgga ttgaggaggt
    32941 tttaaaatgg aaaattcaaa cactaactta ggtagtgaaa gtgtgggtaa attattattt
    33001 aaacttgcta cacctgctat tatcgcacaa attgtaaatg ttttatacaa catagtcgat
    33061 aggattttta ttggtagaat ggaaaatgga gaagttgcta tggctggtgt tggagtggca
    33121 tttccaataa taattataat aactgcatgt agttatttaa tcggtatggg aggaggtcca
    33181 ttagctgcta taaaaatggg tgagcaaaac aatgatgagg cagagaaaat aatgagtaac
    33241 agtttttctg tattggtgat tttagcaata cttttgacta taggttttaa gattggaaaa
    33301 gagcctttac tttggatgtt tggagcaagt gaatctacaa ttggatattc aatggactat
    33361 ttaaatattt atttaatagg tacagtgttt gttcagattt caatgggtat gaatactttt
    33421 ataaataccc aagggtttgc tacaactgga atgatgacag ttgctatagg tgctttaata
    33481 aacataatac ttgacccgat ttttatattt ggctttaata tgggtgtaaa aggagctgca
    33541 ttagctacta taattgcaca aggcgtttct gctatttggg tacttatgtt tctctttgga
    33601 aagaaaagta tacttaaaat aaaaaagaag tatatgatac caaaagcaag tatcatctta
    33661 ccagtgcttg gcttgggaat ctctccattt ataatgcaat ctacagaaag tttagtactt
    33721 atagccttaa atagtaaatt acaaatgtat ggaggagatt tagcagtagg ttcaatggcc
    33781 ataatgagca gtataatgca aattcttatg cttcctaata tgggggttac acaaggagca
    33841 caaccaataa taagttataa ttatggttca ggtcaacttg atagagtaaa aaagacattt
    33901 aagttgtgtt tattgtcatg ttttacatat tctactattt tatggctttt attaatgata
    33961 tttcctgcat tttttgttag tatatttaat aagaatccgc agttacttag tatgactagt
    34021 tggagtataa aaatatattt tgctggtgct tttatgtttg ggattcaaat agcttgtcaa
    34081 cagacatttt tagcacttgg aaaagcaaca atatctttgg ttttagctct acttagaaag
    34141 attgtacttt taataccttt aatatttata cttccaacat tctttaatga aaaacttttt
    34201 gcagtaattt tagcagagcc agttgcagat ataacagccg ctactataac agcaatttct
    34261 ttctttatat tttataaatg ttttctatca aaacctaaag caataaaaga ataagatata
    34321 caatataaat tttttatgaa aatattttat tagataaaaa ttagaatttg tattttaatt
    34381 taataagata ttttctataa tgtataaata tatgaagttg aatttatttt aaaaattata
    34441 gtaagaaaaa tgtaagtatt tgcaatatta atggtataat atttacagat atgataatca
    34501 ataagaggaa aggatgttta taatggaaaa tgatttaaca atgcaagaat tacttgacca
    34561 gcaagaacaa gtattcagca aagtgaaagt tggagaattg acaactggaa aaataactgc
    34621 agtgagaaat gatgaagtac aattaggatt agactatggg tttgatggaa taatacctat
    34681 aagtgaattg aacatagaaa aaaatcaata tatagaagat atataccaca taggtgatga
    34741 aataactgct gtaataacaa aagtatctca aaaagatggt acaataactt tatctaaatt
    34801 acaacttgac aaaagaaatg attttgccga gttacaaaag gcttatgatg aacatagaat
    34861 aataactgta aatgttgaaa agaatataga taaaggtgta tttgctaatt acaatacata
    34921 tacatttttt ataccaattt ctcaacttga tacaaaattt attacagata catctaaatt
    34981 tgttggtctt aacttagaag tttatataaa agaattggat gttagaaaaa atagacttgt
    35041 agcatctcat agagatgtat tacaagagcg tataaataaa gagagagaag aaagaagagc
    35101 tcaaattaag gcagaaaaag aagctgaaag agcaagaatt aagcaagaaa gagaagaaga
    35161 aaaagctcgt atcaaagcag ctaaagaaga cttatttaat tctcttgaag ttggacaaaa
    35221 aagagatggt aaggtgacaa agataatgcc ttatggtgca tttgtggata ttggtgggat
    35281 tgaaggatta gcacatataa ataatttagc ttggacaaga gttgaaagtg ttgaagatgt
    35341 tgtttcagag ggacaagaag tagaagttta tgtgttggat gtaaataaag agactaaaaa
    35401 aattgccttg gcattaaaag acataaataa tgacccttgg gatttaatag caaaagaagt
    35461 acaaattgat gatgttgtca atgttaaagt tcttagaata atagaaaaag gggcttttgt
    35521 tcaaattaaa gaaggtgtag atgcgtattt accaatatct gaattgagtg atactagagt
    35581 ggctaaagtt actaatgttg ttaatattgg agatgaggtt aaagttaaga ttcttgattt
    35641 taaaccaaag actaagagaa tgttggtttc aataaaagaa gcaactagag agccagaaga
    35701 ggatataaca gaatacctag aagtagagga atctttagga agtattggag aattatttaa
    35761 agataaattt aaagatttag aagtgtaaaa taattgaaaa tggggctgtt ttaaaacgtt
    35821 acactgtcta ttatgacagc tttagttttg aaatagccct ttagctattt tcacgaattt
    35881 atgtaaaata ttaggaggaa atttaatgaa aaagtctatt atggatgtac attgtcacac
    35941 tctaataagt ggacatgcac atagtacttt caaggaaaat gtcgaagagg cgagtaagaa
    36001 gaatataaaa tacttaggaa tatcagacca tggacctaat atgccaggag gaccacatcc
    36061 attctatttt tataatttgc atttgcttcc acgtgaagtg caaggcgtaa agattcttag
    36121 aggaatagaa ggaaatataa tggattataa tggtaattta gatgttcaag aagatatgtt
    36181 gcaacatttg gattatataa tcgcaagttt gcatagacct tgtatagctt ctggtacaaa
    36241 agaagaaaat acaaatgcta ttttaaaagt tatggataaa cctaaggtaa aaataattgg
    36301 acatccagat gacagtaggt atcctttaga ttatgaacct atagtaaaaa aagctaaaga
    36361 taaaaatatc cttttagaaa taaataattc atcattaagt tcaaactctc atagaacggg
    36421 tacttgggaa aatgtaagtc atatgttgac attgtgtaaa acatatggtg caagagtaat
    36481 tcttggtact gactctcata tatgttattc tataggagaa tttgaaaatg cagaaaaagt
    36541 tttagaagca gtagattttc cagatgaatt agtcataaat tatcatgaag atgaaattat
    36601 agaatttttt gatataaatt tttagaatct tggaagtgaa aaaatttgag taaaaataag
    36661 tttatctata tgaattatta tgacaatggt tattttaaaa aaggaatgac tatatatatg
    36721 gttttagcac ttataatttt agctacaatt tttatatatt caacgacaat attatatatt
    36781 ttatgtataa taacagcagt ttttctattt aaacaaggtc gtgatttttc aaataaatat
    36841 g
//
LOCUS       DAYAOY010000034        36620 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYAOY010000034 DAYAOY010000000
VERSION     DAYAOY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.034"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            257..1021
                     /locus_tag="ACGI6B_001760"
     CDS             257..1021
                     /locus_tag="ACGI6B_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9751206.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(1302..2417)
                     /locus_tag="ACGI6B_001761"
     CDS             complement(1302..2417)
                     /locus_tag="ACGI6B_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFG9751207.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="ACGI6B_001762"
     CDS             complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="ACGI6B_001762"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFG9751208.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4277..5374
                     /locus_tag="ACGI6B_001763"
     CDS             4277..5374
                     /locus_tag="ACGI6B_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9751209.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(5865..6377)
                     /locus_tag="ACGI6B_001764"
     CDS             complement(5865..6377)
                     /locus_tag="ACGI6B_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751210.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            6930..7811
                     /locus_tag="ACGI6B_001765"
     CDS             6930..7811
                     /locus_tag="ACGI6B_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HFG9751211.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(8124..8903)
                     /gene="hisJ"
                     /locus_tag="ACGI6B_001766"
     CDS             complement(8124..8903)
                     /gene="hisJ"
                     /locus_tag="ACGI6B_001766"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HFG9751212.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(9449..9799)
                     /locus_tag="ACGI6B_001767"
     CDS             complement(9449..9799)
                     /locus_tag="ACGI6B_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFG9751213.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(9815..10231)
                     /locus_tag="ACGI6B_001768"
     CDS             complement(9815..10231)
                     /locus_tag="ACGI6B_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HFG9751214.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(10250..10441)
                     /locus_tag="ACGI6B_001769"
     CDS             complement(10250..10441)
                     /locus_tag="ACGI6B_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HFG9751215.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(10592..11407)
                     /locus_tag="ACGI6B_001770"
     CDS             complement(10592..11407)
                     /locus_tag="ACGI6B_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFG9751216.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(11454..12392)
                     /locus_tag="ACGI6B_001771"
     CDS             complement(11454..12392)
                     /locus_tag="ACGI6B_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9751217.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(12389..13261)
                     /locus_tag="ACGI6B_001772"
     CDS             complement(12389..13261)
                     /locus_tag="ACGI6B_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9751218.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(13286..15214)
                     /gene="acsV"
                     /locus_tag="ACGI6B_001773"
     CDS             complement(13286..15214)
                     /gene="acsV"
                     /locus_tag="ACGI6B_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HFG9751219.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(15326..15703)
                     /gene="gcvH"
                     /locus_tag="ACGI6B_001774"
     CDS             complement(15326..15703)
                     /gene="gcvH"
                     /locus_tag="ACGI6B_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFG9751220.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(15790..17916)
                     /gene="acsB"
                     /locus_tag="ACGI6B_001775"
     CDS             complement(15790..17916)
                     /gene="acsB"
                     /locus_tag="ACGI6B_001775"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HFG9751221.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(17973..18779)
                     /gene="acsE"
                     /locus_tag="ACGI6B_001776"
     CDS             complement(17973..18779)
                     /gene="acsE"
                     /locus_tag="ACGI6B_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HFG9751222.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(18829..20196)
                     /gene="acsC"
                     /locus_tag="ACGI6B_001777"
     CDS             complement(18829..20196)
                     /gene="acsC"
                     /locus_tag="ACGI6B_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HFG9751223.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(20227..21171)
                     /gene="acsD"
                     /locus_tag="ACGI6B_001778"
     CDS             complement(20227..21171)
                     /gene="acsD"
                     /locus_tag="ACGI6B_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HFG9751224.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(21191..21961)
                     /locus_tag="ACGI6B_001779"
     CDS             complement(21191..21961)
                     /locus_tag="ACGI6B_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HFG9751225.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(22017..23402)
                     /gene="lpdA"
                     /locus_tag="ACGI6B_001780"
     CDS             complement(22017..23402)
                     /gene="lpdA"
                     /locus_tag="ACGI6B_001780"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFG9751226.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(23446..24327)
                     /locus_tag="ACGI6B_001781"
     CDS             complement(23446..24327)
                     /locus_tag="ACGI6B_001781"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFG9751227.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(24362..25036)
                     /locus_tag="ACGI6B_001782"
     CDS             complement(24362..25036)
                     /locus_tag="ACGI6B_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HFG9751228.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(25073..25945)
                     /locus_tag="ACGI6B_001783"
     CDS             complement(25073..25945)
                     /locus_tag="ACGI6B_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HFG9751229.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(26037..26669)
                     /locus_tag="ACGI6B_001784"
     CDS             complement(26037..26669)
                     /locus_tag="ACGI6B_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HFG9751230.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(26774..28450)
                     /locus_tag="ACGI6B_001785"
     CDS             complement(26774..28450)
                     /locus_tag="ACGI6B_001785"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFG9751231.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(28699..29478)
                     /locus_tag="ACGI6B_001786"
     CDS             complement(28699..29478)
                     /locus_tag="ACGI6B_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9751232.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(29788..31707)
                     /gene="cooS"
                     /locus_tag="ACGI6B_001787"
     CDS             complement(29788..31707)
                     /gene="cooS"
                     /locus_tag="ACGI6B_001787"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFG9751233.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(32314..33225)
                     /locus_tag="ACGI6B_001788"
     CDS             complement(32314..33225)
                     /locus_tag="ACGI6B_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFG9751234.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(33341..33919)
                     /locus_tag="ACGI6B_001789"
     CDS             complement(33341..33919)
                     /locus_tag="ACGI6B_001789"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9751235.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            34145..35068
                     /locus_tag="ACGI6B_001790"
     CDS             34145..35068
                     /locus_tag="ACGI6B_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9751236.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(35240..36382)
                     /locus_tag="ACGI6B_001791"
     CDS             complement(35240..36382)
                     /locus_tag="ACGI6B_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9751237.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(36400..>36620)
                     /locus_tag="ACGI6B_001792"
     CDS             complement(36400..>36620)
                     /locus_tag="ACGI6B_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HFG9751238.1"
                     /translation="QDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTE
                     IREVENVVLFEYDDKKIFEKSKTHKVEI"
BASE COUNT        11307 a         7069 c         4197 g        14047 t
ORIGIN      
        1 atctctatac taataaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt
       61 tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa
      121 atatggtaaa attatcttgc ataacactta taatattatt tgataaatat acatacatta
      181 acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt
      241 ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa
      301 atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac
      361 tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg
      421 tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa
      481 ctcaaacgat aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac
      541 tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa
      601 gaatctaact gtaataacca actcaatttt agttgcaaat gaacttatga atactaatat
      661 aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct
      721 tacactgtca gctattgaac aatttaatgt tgataaagct attttaggcg ctggaggtat
      781 cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat
      841 gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc
      901 atttgcatca gtagctccta tagaacatat agatactatt attacctctg ctagtataga
      961 ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta
     1021 gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa
     1081 ttatttattt agctccattt atgtctaaac tatactttat attttaatta tgtaactact
     1141 ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta
     1201 gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc
     1261 tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc
     1321 attttaatac tttgttcttc ttccaaggtt aaatcttttg tcataagtat agcaagtgca
     1381 attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact
     1441 actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca
     1501 gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta
     1561 ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc
     1621 actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct
     1681 aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct
     1741 tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc tatcgttatc
     1801 aatatagata taaattttcc aaaaattaat gacccattta tcataagagt agaattaaat
     1861 tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt tataattacc
     1921 attgataagt taaatcctgc tattaatcct cctataatac ctccaaaggg tattgttatc
     1981 aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt
     2041 cctactggaa ttgaaaatac taatgtacat ccaagcatgg atgacacaac caatcctgca
     2101 agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca
     2161 ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt
     2221 attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct
     2281 aaagccaatg gacccattga attaaaccct tcttcaaact gctcgccaag tccaaattta
     2341 tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata
     2401 attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta
     2461 attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttggtt
     2521 ccatccaagt aatatttctt ctcttttttg agtagtcata tttggaatat attttttgct
     2581 tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc
     2641 catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc
     2701 taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct
     2761 caacattttt atttttttcc ctgaggctaa gttcatagaa tcaacaacat ctttgacttg
     2821 aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat
     2881 cccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc
     2941 tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga
     3001 ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata
     3061 cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa
     3121 aataccatta cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt
     3181 accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg
     3241 ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt
     3301 agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag gtactcctat
     3361 aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc
     3421 tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact
     3481 atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt
     3541 atctattacc catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt
     3601 atctattatc ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt
     3661 tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa
     3721 cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc
     3781 attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc
     3841 tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc
     3901 gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg
     3961 gtcaattgct aatacatatt tcactttaaa tgtcctcctt tattttcaga attaataaat
     4021 tcttttttat tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt
     4081 ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt gattgaaaaa
     4141 gtatttttat tgaaatattg ttgcaatatt tattatttta ttctataatc tagttaaaat
     4201 aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt
     4261 ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac
     4321 agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat
     4381 ggatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta
     4441 ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat
     4501 ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga
     4561 agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata
     4621 cccggaccta gacattttag aaaaatacat aattgaaaac tctgatataa aagtaattgc
     4681 tatggtgttc catgaaacta gttcaggaat gataaatcca gtccctgaac taggaaaact
     4741 tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata
     4801 tattgatgtg gaagagttta atatagatat aataacaggt gttggtggca aagctcttgg
     4861 agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga
     4921 aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc
     4981 ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac
     5041 tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag
     5101 gtatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac
     5161 agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga
     5221 aagggaaagt ggctatgttg tctatccagg aaaaggcaaa ttcctagatg ctaacatatt
     5281 ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct tagatatctt
     5341 aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa tcctacaata
     5401 tatcagtata aattaaatag actgattttt ttaaattttc ctataataaa ttatatttta
     5461 tatttttgta taagggtgaa ctataatatg taaagttcac ccctatttca aatctttttc
     5521 cttcaatata atctttttct tttaatttat tttatatttt tgacgcaatc gttttttatt
     5581 caatcatttt atattcttga tacaatcatt cttacactta attatttttt gtttttgaca
     5641 caatcgtttt ttgttgaatc attttatatt cttgatacaa tcattcttac acttaattat
     5701 tttttgtttt tgacacaatc gttttttatt cagtcattta atattttcaa cacaatcatt
     5761 cttacattct attattgttt gtttttgata caactgtttt tattcagtca tttaatattc
     5821 tcaacacaat taccttttca ttcaatcaaa gtaatcttta agcattattt ttcaagctca
     5881 tcaaagtaat tatatacgat aacctcacct gtctctttat ctgtagtctt tataatgccc
     5941 ttagctccat ttacctcttc ttttgtaaat tcatcatcta acacaacttt gaatttatct
     6001 tcctttccat aaccttgttg aaattcatct ctcaaaattg catttttttc tgcttttggc
     6061 ttcaactcaa attcaattcc agatttcatt tcatcaaaat aatttaatac tacaacctca
     6121 ttattcattt tattagttgt ctttataatc cctttagctc catttacttc ttcttttgta
     6181 aattcatcat ccaatgttat attaaatttg tcttcttttc catattcctc ttgaaaatcc
     6241 ctcttcattt cattgtattt agtttcaaac tctttactct tgtttaattc catagctgat
     6301 acatttgtca atgatgctcc aattgccatt actgttaatg ccattactat accactttta
     6361 aattttttta ctttcatatc ttacctccta aattaaatgt tctcaatgaa attatataaa
     6421 gaaaatccta agttttacag tttcattctt gtaactaaaa ttactattat gtaaccaaac
     6481 tttttgcttc aactattcct taaaagttgc ttttttgagg aatagttgaa gttaaataat
     6541 tagattttat ctatatttat agatttaaaa tcaaatttat ccatataaat aaatacttat
     6601 ttttgcaaaa taaaaatttt taattactcc aatccttctt atttacttaa aatcctatga
     6661 taagaaattt aaaagtcttt accttcttta acaataattt atttactttt aacatacaaa
     6721 atatagagag tatatcaaaa ctattttact gaatattcta aaaataagta ccccctatta
     6781 gtatatatat ttacatatta aaaaaaagtt ttaaataaag ttatattttt tacaacagtt
     6841 ttgtaattaa aaacatattg aaggtaaatt taaaggtact atatactata aatatgtcga
     6901 cacaataaat ttaaaaagga gaactaaata tgaaaaaaag attaataata atgatattgt
     6961 ctgttgtctt agtactatca agtatactta cgatatttgc atattcaaat attaaatata
     7021 ataataacaa attgatatat tctaatatga tagataaaaa aactcaaaat agtgtaaagg
     7081 aaatcctaaa agaaaataaa attaatgaaa aagacataga tacatttatt aaatcagtca
     7141 acaattataa taaattgcaa gttaaaatac ttcaaaataa tataaatata tctaaatctg
     7201 gatattcttc aatcaatgca aaacaagttc catataacct agaaaaattg caagataatt
     7261 gggtgaaaaa attccctgat tatatggatg taaattgtag aataactgct tttagacttt
     7321 ttaaagattt tataaattca aataagaaat ttactggaga ctctattgat ttgaatgtcg
     7381 acttggacac tataatgaat aataaagatg ctaaatttag caccaaagat gtagaaaaat
     7441 ttatcaactt cttttctgca ataccagcta aagatacaga tgatacaatc aaaattgcag
     7501 aacaaataaa aaatgaatgg aaaaaaagaa aaatatcttt taaaaataat aaaaatatat
     7561 ctattataaa tggatttctt cgttatccag aaactaaaaa tgtatttata ggtcatacag
     7621 gcatatgtat taaaactaaa aatggcattc tatttttaga aaaatacggt gttacatcac
     7681 cttatcaagt tactaaattt aaaaataaag aagatgtaaa aaattatatg tttaatagat
     7741 tgaagatgtc agaaggtgaa attgaattac ctgaccctat aataatggaa aacgataaac
     7801 ttatgaaata ataactttaa acgcaaaata gtatttataa ttactaagtc aatataatag
     7861 agaaggtcta tagatttgtg taaatacaag cttatctaca atcttatata agaatgttta
     7921 tataaataag atagattaat tctaagtcct attattctat tgaattttat tattatttta
     7981 tttagattct attgttattt tacttagaac ttattattat tttgtttaag ttctattact
     8041 attttcaatc atataaattt acaaaagact gtacataaat ttattttata caaacttatc
     8101 tacagtcttt tgtgtgaata tatctaaata atatcgtatt tatttatttc ctctgcacag
     8161 tgagaatcag aacctaaaac taatggaata tcgtattttt ttaccaagtc taataagtat
     8221 ccatctatat aaggctcttt gcagtactct tttctatttc cagaaacatt ataatcaagt
     8281 tcataacctt tttctttaat taactttatt aactcttcaa gaattttatt tcctttataa
     8341 tcgtatggaa atacctgatt aaactttcta actaaattta aatgacctat tctttttggt
     8401 ttatgtatac ctaaatctga atttacagct tttatcaatg tttcataata cttattataa
     8461 acagcttcaa tacttcctaa tttgtcgatt aaattttgaa actcctctac actgtaatct
     8521 acacaataat aatcatcatc aatcttaatt atatgtactg ataaaagaga atcctctaat
     8581 tcctttccat atttatttaa attctccttt atcccttctt cgtatccttc tataaaatcc
     8641 acttccgacc caacatttat cttgatttta ccctcatact tttctttcaa cttcttgaca
     8701 tcattaaaat actttggtaa atcttcaata ctcatacaac tatcattttc aggagcaggg
     8761 tctctaaatc catttgcata cggaaaatgt tctgtaaatg tcatttctgt tattccaaca
     8821 tccattgctc tttgtacata tttttcaaaa tcatccttac ttccatgagg acaataaggt
     8881 gaatgtatat gaccatcttt cataaataat tcactccttt tttaaagaaa aaatctaata
     8941 taaatgcata aatcttttaa cttcaaaata atatttttaa tcatgctaat ttttattata
     9001 tctaatatta gagtaatttt aaactgcctt ttaagtatct tatcattagt actattttca
     9061 acttaacatt aatattattt tttaacttaa cattaatact atctttaaat agtaatactc
     9121 aatatataat tacttaacat aaaataactc aaattgcttg atgaggcttt tgcagtattt
     9181 aaagaatata taattattta acattaaata atccaatctt gtaataaaat ttactcatta
     9241 aagtatatta cagaaaatac ctcaaaataa actttttagt tcatgatata ctcttttttg
     9301 catgaaatta gatatatttt tatcatttca acaatgaaag ggaggcttat atccatcttt
     9361 agacaacccc ttagataaaa tttttaataa ttaaacatca caaaatataa aaatcgctta
     9421 tatcaaacgt ataagcgatt aattatattt acatatgttt tttagcctct aacaacattt
     9481 tattagcttc ttccatatac tctatagcct tttcaatact ctttgctgtt tcttcttttc
     9541 caatagctct agccttttca acccactctc taaatccttc ttcatgagtt tcattgtgat
     9601 ttatccaatg tactaaaagt atttttaaag ttttttcatc cttagactct gatgattcct
     9661 catgactatg ttcgtggtcg tggtcgtgag catggtcatg tggatgttca tgacatatat
     9721 ctccatgagt atgttcgtgg cagtggtcat ggtcatgatg tccatgatga tatcttacaa
     9781 tctctttatc aaaaatcatt agttattccc cctgttattt tatattaaac tcttttttta
     9841 taaggtttac agaatctttt acaagtagtg ctaatggttt accttctaat ccaactaact
     9901 ccacattttc tggcataata ggtaaaagta tcttaagtgc ctcactgtct gcaatagctt
     9961 cacttatatt acaagtaact tctcccatca tagaattagg tattattaca gaaataggtg
    10021 caactattat atttgctttt ttagctgata ctactatagc attttcccct gtagctcctt
    10081 tattagcatg agctttcatc atagaactag ttgctataga attagtccct agtgcataaa
    10141 tttctacata tgtaggtaac tcttctctca aagaagaaac tatctgggct ccaatacctc
    10201 cacccattcc atctatcact gctattatca tatgaaattc ccctattaat taattttctt
    10261 gtattattat cttatggtcc atcaatctta tttctttaat tatcccatct attatctttt
    10321 gctctcctaa taaatccgtt aaataaatct ttccatcaca agggtctata ttaaccacat
    10381 tttccataac cttttctaac tcattattac ttttacaaat gaaagctgat gattcacaca
    10441 tataaatctc ctcctaatat gatttttatt aaaaatcaaa aaaagattcc cttaggtatt
    10501 tcaatacacc aaagaaacct tcatgatttc agtttttgta tattattata ccttaagtat
    10561 atcactttaa tttatgtaaa gtcaattaaa gttaacgctt tttattatac tcttcattct
    10621 tgtatttttc atatagttct ttaactaatt gcttctcata atcagtcaaa tcgccctcca
    10681 caaactcaac attaaattgt tcagctaatc cctttactat gtttttctgt agaatatcta
    10741 tgtctatttt tctgccaatt tcattttcta ttccacttgc ttttttagct gtaaatttca
    10801 tagctctttc tttcaattct ggtgtcttag tttttattaa tttaaacaat ttttctacat
    10861 caaaatccag tattattgaa ccatgttgta ataaaacacc atcttttcta ctttgagcac
    10921 ttccaattac cttcttatta ttaatagtaa cttcatatga tgcatgtgca ttaaaacatg
    10981 cagctgataa attttcccta cttattctct cgcctctatt taaattatct gtttctatac
    11041 cacttagatt caacccttta actagacctt cacttatata tctataagat aggttgatgc
    11101 tcttatcgat taaaggatta tcttctccta taattatact ataggttagc tcattatcat
    11161 gtaatacagc tcttccacct gtaattcttc tagtatagtc tatattcata catctacact
    11221 tatctaaatc tatctcatct tccaatttct gaaaatatcc tatactcaaa caagctggtt
    11281 cccatgtata aaatctaaga gttggcttat tatgtccttt cttatatgca gtaaatatag
    11341 cttcgtcaat tgccatattc attgctcctt catatgattt attatgtata actctccact
    11401 gattcatgat tattacgcct ccatttttag tataaatata tcctagaaat cctctataga
    11461 tttatttctc ttattgctaa atctatctca ctttgattta aaggtcttga atagttgtat
    11521 atagtacttc ctggtctttc attatagtat ggtctattac aatccttaca acctcttatt
    11581 tcaaaaggat agcctttgtt tatgtctttt aaaatatcat tgtcaagctt tatactattt
    11641 aagtatccat ttttaaactt aaaatactct tttggataac ctttatttat catataagac
    11701 attaactgta cccttctata actttctatg cttggttgat ttatcttttc cattttagtt
    11761 cctctaactg gagtaaatgc aaataaacta atcgttacat cattttcctt taaataagta
    11821 tataaattgt atatatcttc atgactttca cccataccaa ctattatatg tgtacttatt
    11881 ttattgggat atgattttga catttcagtt ataaatttta acttttcatc atagttattt
    11941 ccttttattt tttcatatag ttctttattt gctgcatcta ttgctattcc tattttatca
    12001 actcctgctg aaaaaaactt ctttatctgt tcatcatttt ctaattttgc agatagtgaa
    12061 attggcatgt ctatcttacc acttatatag tttataaagt ctaaaacaga gttatgtgct
    12121 tcttcacttg ccattgattg aatacatatt ctctttatat tttttccttt ataagctttt
    12181 aaagcatcta atatttcttc ttttgaaaac tctggccaaa ttactcttga taatttatct
    12241 tttctagtac tactttctat tgattgagaa caaaaactgc atttatttat gcatttttct
    12301 ccaatcatta tatatgctgt ggtaggaggt atatcgcttt ttttgtttaa tatcccaagt
    12361 tctattgctg tcccaactga tagtctaatc ataaaacgca acactcctta cactcattta
    12421 ttgtcatatt catttctata gccttattct ttgcagacct tgatggtaat actattctat
    12481 tgactccaca cattatagat aattggtcta gctcttttct atacactcct cttggtctca
    12541 tacaacctag attaatttca gtatctggta aattaattct agcttcacat aaaatatctg
    12601 ctaccccttc taactcagga ggttctacat tttcatactc agtccctttt gttggaataa
    12661 ggactataaa tgtaagctta ttaggaggat ttttttgtag gtattctata atttcatatt
    12721 caccctttat ttgtcctcct tttaaaccaa tacatatatg tggtgctact tctgtatgtt
    12781 cttgtattgt attatagact tcaatataat cttctttact ctttttcatc ttataaactt
    12841 ctcttattgt ctcatcatca aacactaaat caaaagaaac tatatctaaa tacttacaca
    12901 actctacaat gctatcttta tccataagac ctaaatgagc attcaatcta tatccttgct
    12961 cttttaagtt tttaatggta ttaatatgag tatttatagg gacatctccc tcataactac
    13021 aaccgccact caatagaaag ctagttatat tccttgattg tactttttcc tcatattctt
    13081 ctattggcac catattgttt aagtagtgac cgttgcaatg ggcacatttc aaactacatt
    13141 gattgcttgt agtactaaga gctagtgtct ggttaggata agcaaactct attttatttc
    13201 caaaattctt taatttaata tcaaatgcta ttttcatttt ttcagataga ttcttattca
    13261 tagtttattc cttcttaagt acattttact cttctagtaa tttctttgct gttgggaact
    13321 gctctatatt cgtatgtggt aagaaacatg cagaaataaa ttcatccata taacttggat
    13381 atacactaag ttcaacataa gttatttgat tacctatttc ttcaagttta ttttttgcat
    13441 ctttacttat aagcgctaag taagaaccaa caagtgaact attccctata taagtaaatt
    13501 tttctctatc aatatcagga agtaacccta taattattga gttttctatg tctaggttgt
    13561 ttcctatacc acctgctata taaaccctat ctaatatact aaaatccata cctaaactat
    13621 ctacaagagt gtatgcgcct gaatatatag ctcctttagc tctgatgaaa ttatctatat
    13681 caacttcatt gactactata tcattttcta aatcaaattc ttctttaaat gctaatacat
    13741 actctcctat ctcatgctca ttaaatctaa ctctcttatt atctaaatcc ctgtatattt
    13801 tacctcttct atctattaca cctttagtaa tcatttgaca gattaaatca attataccag
    13861 aaccacatat accaactggt gcacattcat caataatctt taatgttggc tctaaagtat
    13921 ctttatcaat aattactttc tctattgctc ctgctgatgc tctcattcca cagcttatcc
    13981 ctccaccttc aaaagcaggt cctgctgaac atgcacagct catcatataa tctttatttc
    14041 caaatacaat ctctccatta gttcctaaat ctataaataa aacattttct tcacttgacc
    14101 atatccctgc tgataataca cctgctgtta tatctcctcc tacataactt gcaacagatg
    14161 gagctaagta cacatatgca ctgtcattta caaaaagacc aacattttca cccataagtt
    14221 ttggagattt taaaaatggt ggtatgtaag gttcttgtct taagaagtct gtatacacac
    14281 ccaagaataa acttgtcata gttgtatttc cggcagccac taatgtaact acatcttcct
    14341 tatctattcc atttctttca tatattgttt ttaatagtgg atttatagtt tcctcaacta
    14401 tagctttatg aagtgtctct aatccatttt tcttagttga atatataatt ctatttataa
    14461 catctgctcc atattttatc tgtgcatttc cagaagatgc tttatctact acctcttttg
    14521 agtataggtc tactaaacaa acaactactg aagttgtacc aatatctatt gcaactccgt
    14581 ataaggaatt ttctttattt ccttgttcta tatttatgat tgtaagtttc ttttgttttt
    14641 gaacatatgt tattgtaacc ttaaaatcac tctttctaaa tacagttggc atttttctaa
    14701 gtatatccaa tctaaaatct atttcattat atcctaggtt atttctaaca tatctttcca
    14761 atctatctac atcacttata ttatcatcta aatttggttc ttccatttct atgtatttct
    14821 tttttatatt tgttttaaat tgaaggttat gttcttcaat tattttttta gctctttcaa
    14881 aaatttccct atcctctttt ttattactac cttctatctt cataccatgc atagaagatg
    14941 atactttaga aggaacttca atttctatat ctgatattac ctttgtacaa caagctagta
    15001 tataaccttg ctcccactca tcatctgtta tatgtcttgt tttttctgta tcaacttttc
    15061 cattcaataa ttttacttta cacttcccac aagacacgtt tccattacaa ggagcatcta
    15121 tgaatatatc tgcatttctt gcaacctcta gtaatatatc cccctcatta caataaacct
    15181 ctttattgtt tggtgtaaaa cttactttta tcatgttatc cctcccaatt tgttctatat
    15241 atgataaaag gaaagtatag ctaaatttgc gtgcactcaa attttactat actttcccca
    15301 attataataa tatcttaatc tttaattatt ctaatccagc ctcgtattct ttatcactta
    15361 ataacgtatc aagttcagcc acatcagcta gttttacttt taatatccag ttttcatatg
    15421 ggtcttcatt tatacattct ggctcatctt ctaatttttc attaacttcc aatacctcac
    15481 cattaacagg agatattaaa tcagaagcta ctttagaaga ctcaactact ccaaaatcta
    15541 ctcccttagt tacagtatct tctacttctg gtgtttcaac aaacaatatt tctcctaatt
    15601 gatcttgagc atagtctgtt atacctatat atacaacatc tccatcaatt acttttaccc
    15661 actcatgatc tttagagtat tttaattctg gtaataattt catttcgata gacccccaat
    15721 attatattat tgattaaatt acttaaatca tattaatatt atatcatatt attttaaatt
    15781 ttccatcaat tacattacac tttccatagc taatgctgga tgaccttttt cttctaagaa
    15841 agctaatact ccttcagaat cgtcagctat agtttcatca caaaccatat cacagaagtt
    15901 ttctattcca gtcatttctt taactgtagc atttaattta tcagctacat aatcttttaa
    15961 ttcttttggc atccaaacta ttctttctgg tccaccttct gcataagcga attttttaga
    16021 agatataaaa tgtcttccgt gtcccataaa tcctggagtt tgaactcctc cacctgtcat
    16081 agaagcaagt tctccgaaag tcataccaac tggagtaaca cttgcaaatt ctctgttaac
    16141 tacaacaaat ccatttgctt ctggcattat accacagata cattcgaagc atccacaaga
    16201 agtcattggg tcttctagta tagagtataa tgtaacagat tcaactgcac cttgagatat
    16261 ttggtttaca gtttcattta ctgaattcca tacacctgtt ctatcatcta agcactcgcc
    16321 tttttctatt ggttgacaag gacctgttgg gtctaattct ttagtagcct tagcatctaa
    16381 ccagctaact gctccacaaa gaccaagtct ttcaggagta acaacacaaa cgtgagctgg
    16441 tgcaaatgat tgacataagt tacaagaata gaaagtatca acactttcat caactaatga
    16501 agctaatctt tcatctctag cattgtactt agctattgct tctcctttta attcagaaac
    16561 tttttcagca tctgtaatta tagttatttc acacttatca actactgatt caaattcatc
    16621 taacatctta gcatataaaa cttctcctat atgctcaagt ctaaatcctt tatcaaatgc
    16681 atctttagag attcttaccc aagccatatc tctttgtcca acgtgcatta caccttctat
    16741 atagtttaag aagtagtgga aacgtctttc aagaactggc tcgaaatctt cttgcatatt
    16801 tttaccagct attttaacta ttacagcaag tggtaatctt aatacaccat ctgcatcaac
    16861 ttcatctata tttggcccta ttatttctat cttatggtct tcaacttctg ttgcttcttt
    16921 cttaacaaca agctcccaag cttcagttct gtttccacca aactcagcaa acatatcgct
    16981 ctttctaatt ctctcacctt caaatgctgc tgcaaatgaa actgggatag gtatttcagt
    17041 aacttttatt tttattcctc tagcttctaa tgaagtagct actatcttat cgtaatcttt
    17101 ttgagttaat aggttcattg gaacttcaag aactgtttgg tcagttataa ctgggaatcc
    17161 taaagctatt gctccagcac ctgcagatac aactaattca cttaatggtc caaatgcatt
    17221 aacaaatgca ggaactctat tagctgtgta ctctaataat ccgtttaaat caccaggagt
    17281 taatccaccg aatattaatg ctgctcttac tgcaacagat acaacgtgta taacagaagt
    17341 tacatcatat cctagaggta taactcttag ctcaagaccc atttttactc cagcttctat
    17401 agcttggtct ataactttac caactaagaa agttagtaaa cctttagatt ggtaatcttt
    17461 tataactttt gctgctgatt ctgcatctgg acactcacca agaacaactg caacaccagg
    17521 tatatctcct gtaactaatg gtacaccaag tgatctgatt atagggtcag ttatatgacc
    17581 tgcacatggc tcactatatg gagcatccat agttgaatat tttaatgctt caattacttc
    17641 tgctgctaaa gctgtagcta aaccagcatt aaaagcatgt tctagtaaat gagttctatt
    17701 tattaaagat tttactactt ctaaagcacc ttctaagtcc cctaaagtgt tcatcttttg
    17761 tcctgttgca gcatatatac aaggtaatga atatgctgta tcagggaaag caactttatg
    17821 ctcttttcca ttcttttcta tagcttcagc taacatagct tgagccgcac ctaaagcttg
    17881 ttctgaccct gtaaagatta tattatatag attcattcta tttccccctt taatatttac
    17941 acctattttt tattaactat accctaaaaa ccctagaaag caaatccacc ttcatttaac
    18001 tctaagaaag agtctgcata taaagatgcc ccgttgatta tgtcacaaga ttttacagtt
    18061 ttcattaatt ctgggtcaca tggattcatt atagctgaac taaatccatt agccattgcc
    18121 attgctaaga atgcactatc taaaacaggt ctaacatgct taggacatcc attagatacg
    18181 ttagataatc ctcctgtagt taaaagtccc atctcagtca tcatttttat agcttctaaa
    18241 acttctactt gtttttcttg catacctttt ataactaagc ataatgggtc aaatagtata
    18301 tcagttggct ccattcctaa cattaagcct ttttctaata tttcttggca gtaagccata
    18361 cgctcatcat tatctcttgg gataccttct tttgcacata aacctataac cattgcttcg
    18421 tattcagctg ctatatctat taaatcaaat cttgagccag catcagcaga gtttattatt
    18481 ggttttgcat tagttctatc ataaacttta agtccagctt cgatagcttt tttattagct
    18541 gtatctaatg ctataggaac attgttgaac tcagattgaa gtaatttgat tccccatgtc
    18601 attatttctt cgccatctct ttcggcaggt cctatattaa aatctatata gtgtgctcct
    18661 gcttctaatt gctcttttgc tctttttaag attggagctg ggtctctttc tgctaatgct
    18721 tttcttattg aaggtgatat acaatgtatt ctctcaccta taatcataaa tttttccatt
    18781 tataatgtcc ccctttaaat ttaatattaa tagttagtta atattttatt agtttgtttg
    18841 gcatgctttt tcttggtatc cttttaagaa cttaggtaat tccatagatt cctctggtcc
    18901 tataactaca ttccatcctg gtaagttatc ttctatatca cctttaagta ctgcaacttt
    18961 tcctggaatg ataaggtctt tacaattagt tacttcttct accttacttt ctttaatgaa
    19021 agcagagatt gagtttccac caaatttacc agcagcccaa gatgtaagaa ctgaatatcc
    19081 acctgcatct ggtattacta accatactgg aacttttgat ctttctatat caccagcaac
    19141 tatgaagtaa gttaatgcaa agtcaacagt aactaatact ggagaatttt catctggatt
    19201 gttgattgga taaatctttg gttcaactct cattggtctt tgtgggtcag tgtatatgtt
    19261 ttgtcttagt gcaaataatg gtaatgcctt agcataacta atatcatcta ttactattat
    19321 agaaccgtat tttatagtga atattgatga taaagcaact tccatcatag ggtttgaatt
    19381 agataatcta tttgcaaata ctattgaagg atatccaaat gttctatctt gctcttttaa
    19441 agctgttctt ctaacttgta tagcattagt gtaagtatct tttatgtttt ctcctgttac
    19501 atctaatact aattctttat atccagcggc ttgaattaat tcaactgttt catataattc
    19561 ttctaagcta ccagctttta cacctaatgg taaacttgct cctttaacta cttctatcat
    19621 atctttgtag ttctctttag ttgctccata taccattggt ctagcatctt ttaatacttc
    19681 aacagcttct ttaactactt gtgcatcatc acaagctaaa atataagcta cttctaatcc
    19741 actgcctttt attttatcta ttaattttaa atacgcatct ttatctccac aatactctaa
    19801 aacagccatt tcaactttca tttgttcacc aattcttaca tagtcaactt ttttcatgtt
    19861 agctatttta gcatctactg cttcatcact catacaagta cagaatgaaa ctgcatatct
    19921 atttctactt actaatgttt tttcatgtct aaataatact gtttctccac ctagttcata
    19981 ctcagatgca ccagcaccaa cttttaaagc tttcattaaa ggtgcagtag cctctgataa
    20041 ttttgctata gcgtcatcag acatatgtgg gcatttacct acttctacag ctcctgaagc
    20101 aaccttcata gagaaagcca tacaagttgg gaatccacaa tccttacagt ttttctttgg
    20161 tgttaattta aatatatcta aagcttttaa tgccattatt ttttccccct aaccttaaca
    20221 atttaattaa gctaatgcgt taaccaattc ttttatagtt tctattgatt ttggatgacg
    20281 aagtataact gcgtttgaac cacctactaa aacacttgct gcagttgaaa cttccattgc
    20341 tatagttctt tcttctggac aaccccaatc tggctcatct tctattggag ctatagcttc
    20401 tttaacatga cctacttcaa aagctactgg tgttatgata ggcatttgta atgtcttatc
    20461 attttgacca aatgctgcaa gtctaattct atccatagta gatgcaacgt actcatatcc
    20521 ataaccaact gctgaacatc caacattcat aactatattt tcacctttaa cgcctagttg
    20581 agttaacaat acgtttaatt gtttagctag gtttatatca acagaagatt cagctcctac
    20641 tttatgtgca taagccatac cagctgatgc tcctactcct ttgtagttat cttctactgc
    20701 tgacataaat aagcagttat gtccatccaa tgtttgagct aatttttcaa ataatttacc
    20761 atctttttca tgatttcctg aaccagctac aactaaaggt aattttattg cttcaattac
    20821 tgccttagct acatcagcac attcatcaac tgacttatct aatccatttg ggtcagaacc
    20881 atcaaatttt aaacaaataa aatctgcttg tgtattagct tcaacatatt tagcccactc
    20941 tactggacaa ttagctacat ctttgtataa ttccttatat gaatcagtcc agctttcagg
    21001 ataaacgtct gatatttgta tacctatttt tggagaatta cctacttctc catcaaaact
    21061 ataaaaaggt aatacatttt ccccacctaa tttaattgct ttttccccta ttcctacttc
    21121 aacttctgat atttttccag aatatttttg agtagacatt ttaaatgcca tatttttttc
    21181 ccccttaagc ctattttaat tctaatttag caaaaatttc tttcatagcc actttagatt
    21241 ttgaatcttc aggtaaatta actaaaggaa tacctgtaga atcatattca tatatcaatt
    21301 catccatagg tacaacaccg attagctcta ggttatgctt ttcaatttct tctttaacac
    21361 catcattcat aaccccattt ggaactttat ttacaatcaa aagtattctt ccaacactta
    21421 actttagttc ctcagctaaa tctcttattc tagcaacagc ttgtatgcta cgtcttgagc
    21481 aatcactaac caataaaagt gtatcaacat gtctagtgac ttttctactt agatgttcca
    21541 taccagcttc gttgtccatg actaagtatt tgtaatgacc agatattttg tttacttgtt
    21601 ctctaagtat cccatttaca aaacagtaac atccttcacc ttctgaccta cccattacta
    21661 ataagtcata tccttcgcct tcttcgatta tagaatttaa tctaaattga agatattgtg
    21721 cctttgtcat accaccaggg aatgcattgc ctaacttttc tctttgattt acttcttctc
    21781 ttatttctcc tattgttgct tcaacttcga tacctaacac ttcatttatg ttagcattgg
    21841 catcagcatc tacaactaat actggtccct ttttgtcctt aaccaaataa tcaatcaaaa
    21901 gccctgtgag acttgtttta ccagttccac cttttcctgc aacagctata ttgtatccca
    21961 ttttaagact tcccccttaa atctagccac agagccttat gtactctgtc tgtctattat
    22021 aatttaggta cagaatgaac acattctcca tgtacatcat gaagtgcttc cattagccct
    22081 tcagataaac ttggatgtgg atgtattgca tcccctactt gttctactgt taatcctaga
    22141 tgaacagcaa gtgacaactc tgtcaataaa tctgtagcat gaggtcctac tactgctgca
    22201 cctattatct tatcagtttc tttgtcagct ataactttta caaatccttg gaaatgtcct
    22261 atagcttgtg cttttccaag acctctaaaa tcaaattgac ctacattgta ttctacacct
    22321 tcagcttcaa gttgcttttc agtcttacca acaccagcaa cttcaggttc agtataaaca
    22381 catcttggaa ttgctctata atctactacc ttagtcttac ctaaagcatt ttcaactgct
    22441 acgatacctt ctttagaagc aacatgtgct aggaaaggag tatctattat atcgcctatt
    22501 gcgtatatac cttctacatt agtttcaaga tgttcattaa ctactacttt tcctctttcc
    22561 atttcaattc ctatatcttc tactccagaa ttgtcaaggt taggtcttct accaacacat
    22621 actaatgcat attgtgcttc tataaccttt ccattagaaa gagttgcaac agccttacca
    22681 tcaactactt cacaagtttg tactccaata cctgtaataa ccttaatttt atctttctta
    22741 aattgtctaa gtagttgttt agctacatct ttgtcttcat ttaatagtat ttggtcaacc
    22801 atctcaacta tagttacttc tgttcctaag gctctaaaga actgccctat ctcacatcct
    22861 ataactccac cacctactat taacatagac tcaggtatct cttcaagtcc aagaacttca
    22921 tcactagtta ttacaacttt tccatcatat gggaacattc ttggtacaac tggaacagaa
    22981 ccatttgcta gtattatctt atctgctttt atagtttcta ctgttccatc atctttagtt
    23041 acttctatag tgtttttatc aattaatttt ccaaaaccat taactaagtt tactcctctt
    23101 ttttcaaata agaactctat accacttatt aattggttta caactttatt ttttctttcc
    23161 attatggcag tgaagtttgg cttaattgta ccatctattt ctataccaaa atcctttgct
    23221 tcttttacag tattcaatac tcctgaagaa gcaagaagtg ctttagttgg tatacatcct
    23281 gcatttaagc aagttcctcc aactcttctt ttttcaacaa ctgttacatc tgcaccaagc
    23341 atagaagctt ttatagctgc tacatatcct cctggtccac cacctactac tactatcttc
    23401 atctttaatt cccccaaacc taatataatc tacaatcttg caatactata aacctgcggc
    23461 atccagtata gcaggtatat tctcttctgc cccaattgcc attatatgaa caccatcaca
    23521 taaatcttct tcttttaatt gtttgataag ttcccctgcc atttttattc cttcacttac
    23581 ccagttttct tttccagctg ctcttaatct ttcgatttgt tcatctggaa caaatattcc
    23641 tggtacattt gcagtcataa atttagccat tcctggtgat tttaaaggta ctattcctgc
    23701 taataccttg caatccatat cccttgttaa ttctctaaac tttctcattg tatttatgtc
    23761 ataaagtgct tgtgtttgga aaaatttagc ccctgctttt attttctttt tcatttttaa
    23821 taattgaact tctattggag aatattctgg agttactgat gctcctagat aaaaatctgg
    23881 agaacctttt aacttattac cagccatgtc cgtacctgcc attaaagtct cagcagtttg
    23941 aagtatcccc acagaatcta aatcaaacac tcccttagct tgtggatggt ctcctacgct
    24001 agtatggtct cctgttagtg ccaacagatt gtttatccca aatacacctg ctgacaacat
    24061 ttctccttgt atagctattc tatttctatc tctccctgtt atttgaataa ctggctctaa
    24121 tccagcatct tttaataatt tacatgtagc aagagaagtt gctctcataa ctgccgattg
    24181 aaagtctgtt acatttgctg catgaactct tccaactaac ggctttgcac attctattaa
    24241 atgtgaaagg tcagtccctt ttggaggagc catttcagta gtaactgcaa attttccact
    24301 ttctaatgtt tctcttaata agctcatctc taatccccct tttaaccttc taaaaaaact
    24361 tttatgaatt tgctgctgct tctttctttt tctttgcact taaacttctt ggattatttt
    24421 gcttagaata gtcttttggt ggtcttattt ctgctaagtt atctaattgt cctatagctt
    24481 ctagtctttc atatatcttt atccaagcac aatcattctc tgagtttact tcacacttac
    24541 catttttagc tccaccacaa gccccattca taagaccttt agcacacata gttactggac
    24601 atatcccacc tgtccaacca agttcacagt ctccacaagc cttacaggct tcttcatatt
    24661 ctccaactct ttgtacttcc cctataaaca aagtattgtt ggctggataa actggcttat
    24721 cttttaaatt cttaactatt gtctgtgtac catctccaca tgctaaagat aaaactgcat
    24781 cagcttcctt agtctcttct tttagagcct ttaaatcctt tttagacttt aaaaggttac
    24841 aagcagggtc aagcattaca tatcctaaga tttttttgcc ttcaccttca agagtttcct
    24901 tcatttttaa aacttcttct tccccaccac ttttacaagt tgctgcacat tgattacaac
    24961 caactaaaac cagcttgtca aaatctttta agtaccctag cacttcttct aatggtttat
    25021 tttcagaaat tatcatatct acttcccccc taaaccttcc acatatatat atctatttat
    25081 tgttttgtaa tttgcaagcc ttaactacat gttttgcaag tatagatgtt gtaactgaac
    25141 caacccccgc tggaactgga gttatcattg atactttgtc taaaactgca tttgtatcaa
    25201 catctccaca taaatttcct tcttcatcaa catttatacc aacgtctata actaccgctc
    25261 catctttaac aaaactttca tcaaccatct tagctctacc aatagctgct attagcacgt
    25321 ctgcctctgc tgctacacct gatagatttt tagtttttga atgacatatt gtaacagttg
    25381 cgtgttcact taataaaagc attgatactg gctttccaac taccattgat cttccaagaa
    25441 ctgttacctt acttcctttt aaatcaacat tatagtgttt taatatctca actactgctg
    25501 taggtgtaca tggtggaaat ccacttttat ccccttccat taccttagca gagtttattg
    25561 gactaaaaca atctacgtct ttttccggtg caataacata ttttataact tcttcattta
    25621 agtgttttgg aagaggtcta aaacataata taccattaac atttttgtca tcattaactc
    25681 tctttaatac atcaatatat tcctcttgtg ttatatcctc agctaactct aacacttctg
    25741 ttgttatacc tatgttttga catctcttta gtgctcctct ttcataagat aaatcatcac
    25801 ttcttgcccc aactctaact attgttagtt taggatttat accttcttta attagtaaat
    25861 cgacttcttt tattaattct tcactaatct tatcagctac aggttttcct tttataattt
    25921 gtcctttagt tgacattcct tccatacatt cacaactccc ctacaagcaa acttaaatta
    25981 atttattcta tataccacat attaataaca tagtatctac tttcgtcatt taaaaattat
    26041 tttccaagag ctttttcaac tttggcataa acttcatcac aaatttttac accatcttca
    26101 actaacttat ctacttctgt ttttacctta ttaacatatt ctttatcttg tatagaattt
    26161 atatttataa ctacatttaa ttgtccacca agtatagcag ctcttaaaca ctgaactcct
    26221 acaccaacat cacttatagc aagtcttgaa cctttatcaa ctaaatcttc atgtaactta
    26281 atagcttcat agcaaactct tactatgttt aatggtactt cacaagcaac ttttaatgcc
    26341 ttttccattg tttctgcttt tattcttttt tcttcttctg tttctttagg aagcccgtaa
    26401 gcttttgata atggtaagaa acattctgca tcatcatcta tcatttttaa taaatctttt
    26461 tctaactttc ctgccttact taatatctct tttacacttt cttcatactc agcatacttc
    26521 ttcttcccta ttgtaaggtt acataccata ctacctaaag ccatacctat tgcacctact
    26581 agtgcagcag ctcccccacc acctggtact gcttctttgg aagatagtac ttctacaaaa
    26641 tcaacacaag ttttatctgc tattttcatg ataatcctcc ttgaaaggat gtaggaaaat
    26701 atctcaaatt gttcgagata ttttacgaac tattttaaga tatctcctac tctttattta
    26761 taaattaatt tccttagaat aatccagcta ttactccatt ttcatcaaca tctatttttt
    26821 ctgctgctgg aacttttggt aatcctggca tcttcattat ttctccagta agtgcaacta
    26881 taaatccagc tcctgctgaa attgttgctt gtcttacagt tattctaaat cctgttggtc
    26941 tacctaattt agtttggtca tcagttaaag aatattgagt cttagccata catactggaa
    27001 ctttagtaaa tccaagtttt tctaagttag ctatttcctt atcagcttca ggagtataat
    27061 ctacaccatc tgctccataa atcttagtag ctatagcatt tatcttgtct tttatagata
    27121 aatcatcttc atagcagaat ctaaagttat tttcttcttc atccaataat cttaaaactt
    27181 ctttagctac tgcaattcca ccttctccac cttttgccca tacttcagaa agagcaacat
    27241 taacacctaa ttccttacac ttgtcttcaa caagtttaag ttcagcttca gtatcaagtg
    27301 ggaatctgtt gattgcaaca actgctggta atccatatac ttgagttatg ttttcaacat
    27361 gttttaataa gtttggaagt cctttttcaa gagcttctag gttttcgtta tttaagtctg
    27421 ctttagcaac tccaccattg tattttaatg ctcttacagt agcaactatt ataacagcat
    27481 ctggttttaa gtttgccatt ctacatttta tatctaagaa tttttctgct ccaaggtcag
    27541 caccgaaacc agcctcagtt attacataat ctgcaaagtg catagccatt cttgtagcta
    27601 taacagagtt acatccatga gctatatttg cgaatggtcc accgtgtaca aatgatggtg
    27661 ttccttcaag agtttgaact aggtttggtt ttaaagcatc ttttaataat gcagccatag
    27721 caccattagc ttttaattgt ctagctgtta caggttcacc ttcgtagctg tatccaacta
    27781 ttatgtttcc taatctttct tttaaatcag atatatcact tgccaaacag aacgctgcca
    27841 ttatttcaga agcaacagtt atatcgaatc catcttgtct aactgctcca tcaccttttt
    27901 tacccatacc atcaacgata tttctaagtt gtctatcgtt catgtctaca catcttctcc
    27961 aagttatttt cttagggtct attctaagtg catttccttg gtgaatatgg ttatcaagca
    28021 tagcagctaa aagattgtta gcagctccta tagcgtgaaa atctcctgtg aagtgtaagt
    28081 ttatatcttc cataggaaca acttgtgcat atcctccacc agctgctcca cctttaacac
    28141 caaatactgg tcctagagat ggttctctta aagcaactaa tacatttttg tctagtttag
    28201 caaatgcatc agcaacacct atagtagtag tagtttttcc ttcacctgct ggagttgggt
    28261 ttatagctgt agttagtatt aacttagctt ttttaccagt ctctcttttt agtagattgt
    28321 agtcaacttt tgctttgtat ttaccataaa gttctacatc atcttcacct agtcctaatt
    28381 tttttgcaac ctctctaata tcttgaggct tagcttcttg agctatttca atgtcagatt
    28441 taaatcccat aactgaatct cctcccacaa cctaaataga tttaaaatat aatttttgta
    28501 aaaattgtat taatatgtaa aaattatatt ttataagata attatacatg taaaattact
    28561 taaaaactag ctactaagct attaaaatat tcaaacttaa tagctagtta tttatctaat
    28621 tacttactta ttacctatct attgcctatg tctaaccttt aaccaactta aaatcccaaa
    28681 tcaaaatttc tatctatttt atttatcctt aagtgacatt aatttatctt ttattgcttt
    28741 aatctgttct ttcgttgttt cactagaatc atatggagat tgattagctc tatcagaatc
    28801 aataacatct tggttataat aaataaatcc taaagattct ccatcttcta agttttggat
    28861 tataaattct tcatcttcct tatttctaat tttatttcct actacaaata ctttatttac
    28921 acctatatca tgtccaagtt tcttaacttt tctataagtc tgtagacttc tctctcctgg
    28981 ttctacaact actataaaag catctacacc ttgggctgtt cctcttccta agtgttcaat
    29041 tcccgcttcc atatccataa ccactacatc tttattttgt agtattaaat gtgagcagag
    29101 tctcttcaaa agaacatgct ctggacatac acatccagta ccaccagaat ctacggttcc
    29161 caatgtaagt aatcttactc cattgtattc tttacagaaa ttttcaggga tatcgtctac
    29221 tttaggattc atcttaaaca tttttccaaa tgaacctaca gaagcagctg ttctatctga
    29281 aactaatttt ttcatttctg atataggtac tattgattca tatacttctt ttggaaaacc
    29341 tagtgctaaa gctaaattag catcagggtc ggcatcaaca gcaacaactc tgtatccgtc
    29401 ttcagcaaac atcctagaga gcattgaaga aaaagtcgtt ttacctactc cacctttacc
    29461 tgttattgct atcttcataa tccctaccac cttttattac tatttgcttt ttagtagtta
    29521 attttacagt taaatatttt ttatactcta tatttttata cactaaaaat aaactacagc
    29581 tagtccttac aggaaccaat taaatttacc ctactagctt ttcaaagcag catgtatagt
    29641 tttattattc tttaatattg tttatatttt atttattcag tatattcatt atattttgat
    29701 gttctttact ttaatatttg actttattca actttattca accagaaact tcttataaaa
    29761 acgtttattc tataacttca ctatttttta agcttcagcc ttttctgcca tcttttcttc
    29821 aactaatgct tcaaagtgaa cacgtttttt ctctatatct tctattattg tagctgctaa
    29881 ctcttttgga tttgtattta cagtaaatgt agctccaact ttatctttta attcactagt
    29941 taatatatcc actgctcttg aagaaccagc tattggaggc attattccta aataagtgtc
    30001 tataccagaa gctacaacat agcatcctat agcaactgct ttttctgaca tccactctgg
    30061 agctatacct acaactggaa ggtcacacat atccatatct aagtaattag ctgctgctcc
    30121 aactaaatct agtatacgac ttatatcaac acaagaaccc atatgtagta ctggtgggat
    30181 tcctactaat ttacagacag ttgcaagacc tttacctgca tatttttcag ccgcatcaga
    30241 ttccattaaa ccaaacttag ctgctgctga agctccacaa cctgtagtaa ctactattat
    30301 gtcgtttttg attaattctt tcattattgt tacgtgagcc tcatttgaaa ctcctttagc
    30361 attgttacat ccaactacac cagctgctcc tcttaatact ccagatttta aacagtctgt
    30421 taatggctta actgttcctg ctgggtctat atgagagttt acaactctgt ctaattgatt
    30481 tactactgct tctacactat acccaactgt tgcgcctgat tttaattcag gtataagtac
    30541 ttttgattta tctctatttt taaagtttaa aatagcttct ttaactattg ctttagcatc
    30601 atcaagtgct tgttcttctc taaattcaat ataagttgaa cctgttatct tagcttttgg
    30661 agaagttgtt acaaatttag tatggtaaca atctgcaact ctagctaatg cagggaatat
    30721 acattgaacg tcaactatta cagcttcaac tgctccagtt attattgcaa gttcttgttg
    30781 atggaagtct cctgctattt ttacaccatg tctcattgta atctcattac ctgtacaaca
    30841 catacctgct aagtttattc catcagcacc aacttcttta gctaaagcta ctaagtctgg
    30901 ctcttctgat gctaaaacta tcatttctga taatgctggc tcatgtccat gtaatattat
    30961 gttaacttta ttttcttcta atactgctaa attcgcctct gttcttcttg gcactggagt
    31021 tccaaataag atatcgctta gacgagtacc tatcatagaa ccaccccaac catcagccat
    31081 agaagttcta agactcatgt gtattaaact atctatatca ccagtacatc ctatatgagt
    31141 agagtgcatt attgttgcaa tttctctatc tatagctctt ggctctatag cgtattcttt
    31201 ccatattttt tgtcttggtt ctggtgcatt ttttataaat cttaatgtcc caaaaggttt
    31261 tccaaattcc attaatgcaa tttctgccac ttcatgtgct acatcataga tatctctacc
    31321 ttctgtctct acatcccatt ctttagctaa agtaattaac ttagcttcgt ctcttatttt
    31381 atagtttcca tttggatcag ctaatgctaa tgtatgtgct atatctctag catggtctga
    31441 atgggcagct gtcccaccag caaccattct tgcaaagttt ctacctacta tagtatgctc
    31501 atctgctcca cataaccccc ttggagtctt agggcttatt ctacatggtc ccattccaca
    31561 tattctacag caaacacctt gaagaccaaa accacattgt gccttaaagc cttcttttct
    31621 atcatacatt gtttctacgc cttcttctct agctttagct atcatcttct ggctgtttaa
    31681 atcaattgtt aacatttttt catccatatt cctatcccct ttttttgatt tttaaaatcc
    31741 cccaggtgac tgacttcatg tcataaaata ataaaataaa tgctttctaa gttgattata
    31801 tactaatttt cagtgctttg ctttagattt aaatattttt ttaacaaaat tagataatac
    31861 ataaaaattt tattttctac acttaattta agttgatttt atcgcttgtt tttggctatt
    31921 tgttaatatc taaacctact tttttgatac ttcagtttaa ttaaattatg ttttgatttt
    31981 tttaaatcaa atttgaaatt tttctagtca tttttatatt attgcagatt gtttgtgtat
    32041 ttattatctt tcactactgt ttggacttat tacagatttt tactccctaa acttaattag
    32101 taaaaacttc ttagaatagc attttttgga ctaatttttt tgaatttttt tatttttcct
    32161 actttaatat taattatagc ttcaaattga acatttgtat atattcattt gaagctataa
    32221 ttatttttta tataaaactt ttttaataca gcattaattt agacattttg actatttttt
    32281 ctactttaat tcatttattt caagcgattt atcctatttc atatttttag ctacaatagc
    32341 cccatatgca atagaatata atttagattc atgtgaatca gctcttgaca ctaaaactat
    32401 aggagctttt gcacccataa ttatacctgc tgatttagag tctgccatat atgtaaaggc
    32461 tttccctatt ccattaccaa cctctattgt tggaaccaat atgatatcta catctccaga
    32521 tacttcactt ttaaaccctt ttatctcact agcttctttt gaaacagcta aatcaaaagc
    32581 cagaggacct tctacaatta cattttcacc aaattctcct tctttacaag cttttgctaa
    32641 catatctgca tctacagttg cttgcatctt aggatttact ttctctttag ctgccaaaca
    32701 tgcaactttt acagtttcca ttcctaaagc ttttgcagct tttatagaat tttttaatat
    32761 ctttgccttt tgttcataat caggagctat attcatacct ccatctgtag ttataagtaa
    32821 tttatggtat ttttctaact catataccat aacatgactt aataaactat cagttctaag
    32881 accatagtct ttatttaaca cttcttttaa tatgacagat gtatctaata atcctttcat
    32941 tacaaagtcg gcttctttac ttgatactaa tttaactgct atttctgcac actctttgat
    33001 atcatcctta tccactattt tgataccact taaatcaaat cctatttctt ttgatatttc
    33061 tcttattttg ccttcatttc caaccaatat aggagtaatt atatttcttt ctacagcatc
    33121 ctttattgcc attaaaactt ctttatcatg agctgctgct acagagagta caattttctc
    33181 tcctccctta agttgttcta aaatatcatc taattttttt atcaaaagta acccctccct
    33241 tatatatatt taactaaata ataccatatt tgctatacct tttctcaatt tattttttat
    33301 gttataaaga atttatataa ctaaaagaca gtttaatatt ttattctggg atttcatcaa
    33361 taactcttaa aagctttgca ggaacaccac ctacaatagt attttctgca acattttttg
    33421 ttacaactgc tccagcagcg ataattgcat tatctccaat agtaactcct ggaactacag
    33481 ttacattagc accaatccat acattttttc ctattgtgat tggagaagga taagttgtac
    33541 cacgtttctc tggtgcaata ccatggttaa gtgtagccaa taccacatta tgcccaattt
    33601 gcgtattatc tccaatctca atccccccct ggtcttgaaa atggcaactg ctatttataa
    33661 aaacattttt tcctattgtg atgttttttc cacaatctgt ataaaatggt ggaaacataa
    33721 aaaatgattc atctaccttt tttccaatta actcagaaaa taaactacgg acttcttctg
    33781 gttcatgata agaagaattt aacttacttg tgattttcat tgcttcatta ctcaacttaa
    33841 ccataaattc atgtatttct gatttactag aaacaggttt tctttgattg atatgtgtta
    33901 aaaattcatt gatattcatt ttatacatcc tttcaatatg tgctttaatt tatttgtaca
    33961 ttctaaataa tattttaata aaaattaatt taatttattt ttattaaaat atcattgctt
    34021 cttttagtgt aatatacaat tatatctata tcaaatatct atagtaaata cttaactata
    34081 cttaaaaagc atataaattg atataattat ataatagata agttaatatt gaggagggat
    34141 aattatggaa ataagagtat taagatattt tttagcaatt gcaagagaag aaagtatatc
    34201 tggtgcggca acctttttac atataacaca accaacactt tctagacaaa ttatggaact
    34261 tgaagaagag ttgggagtta aattattggt tcgaggaaat agaaaaatca ccttaacaaa
    34321 tgaaggtagg cttttacata aaagagcaga agaaattata tcattagttg ataaggttga
    34381 attagaattt gacaatccca atgagattat tagtggtgat atatatatag gaagtggtga
    34441 aactgaagct atgcgactta tagctagaac tgcccatcat tttcatcaaa aataccccaa
    34501 tgtaagatat caccttttta gtgggaatgc tgatgatgtg actgaaaaat tagacaaggg
    34561 actgcttgat tttggtattt taattgaacc tgcaaatata cagaagtatg attatgcaca
    34621 aattcctgct actgatgtat ggggtttact aatgcgtaaa gatagtgccc ttgcatcaca
    34681 aagcacaata aatccaaaag atttatgcaa tatacctctt attatttcaa gacagacact
    34741 tgttgataaa gcaatttcta aatggcttca agacagtaat gaaaaactaa acattattgc
    34801 aacatacaat ttagtttata acgcttctct tttggtagaa gaaggtctgg gctacgctct
    34861 gtgtttagat agactcgtca atactacaga aaacagtaat ctttgtttta gacctctatc
    34921 tcctccttta gaagctaaat taaatattat atggaaaaaa catcaaatat tttcaaaggc
    34981 agcagaaaaa tttttacttc aaatacaaaa agatttttct tctatcatgt tggatacatc
    35041 taagttggat acacctaaag aaaaataaat cataataaaa ggtgagagtg tctcaaaatg
    35101 aactttgcac tgtataaggc actctttttt atacaaagta aaatatatgt tcatcatttc
    35161 agcactaaaa aagaggctta tctctatttt gagatagccc cacttaacca ataaaattaa
    35221 atacaactta acaatatcct tacttattta ttctaagttc tcttagacaa gcaatagtaa
    35281 tttcatcttg tggatttaat tcataagctc tttctatata gtaagttgca tcatcaatat
    35341 caccattatt taaataagcc attcctaaat tgcaaagtat ttctgggtct tcctttatct
    35401 tagccgcttg ttcaaaatac tctattgctt tatctaaatt attcttataa gcctcacaaa
    35461 gccccaattc cactatagta tccacttgat taggctttat tatcaatatc ttctcaagat
    35521 acatcttagc ctgctctatt tcgcccatac ccttatatcc aagtgctatc ataaacagca
    35581 agttccacca atccatatgt tcttcttcta atggaagtaa cttctctaat ccttccttaa
    35641 acttaccttg gaatacaagt gtatatcctt cttcatattg aactttaaag tccatcttgc
    35701 ccagattctc ttgtacctca gctactaaat cagcatctaa ccctagcctt aacgcttctt
    35761 cccaagtcag cttagtcttt aaatattgac cttgattata ataatggtag cctaagtgat
    35821 aataacctaa tgcaaaattc tcatcaacat caattacctt ttcaagctta gccatggctt
    35881 caagtaagaa tgcattcatt gccttatcat ccatatcttt ttgatactgt ttagctatct
    35941 cttggcaaac aatcgcatag ttatacattg catctaaatc atctggatac attgttataa
    36001 atgcttttat atatattaaa gaatctgtat gttcaccaat ctcaaagtat tttctcgaca
    36061 tatatcccat atacgatttc aaatccaaat caatatcttt tgataaagca tttaaaaact
    36121 tctcatactc tgcattgttt ttaaacttac tatctattcc aattatatat atcattgcat
    36181 ctgctataga cattgaattt aacttatctt gagcagtctt ttcttttatc ccttttacta
    36241 atacctcatt ttttataggt acatctaatc caccttttgg cacttcatag ctttttaatt
    36301 taaactctcc accatgtttt atagttataa aagctaattc ttctgctttt tttagcaaat
    36361 atttttctat cttaaaatcc attttatcct cctaaatact taaatctcta ctttatgagt
    36421 tttagatttc tcaaatattt ttttatcatc atactcaaat aagactacat tttctacttc
    36481 tcttatttct gtatttatat cattttttaa atccacaaca ttaaatctaa atggctcaaa
    36541 atgaacatat tttattatat gtttagctgg tttatcaaca aaactaggta aatatgttga
    36601 taagttatca ttatcttgta
//
LOCUS       DAYAOY010000035        36328 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYAOY010000035 DAYAOY010000000
VERSION     DAYAOY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.035"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(228..1499)
                     /gene="sleC"
                     /locus_tag="ACGI6B_001793"
     CDS             complement(228..1499)
                     /gene="sleC"
                     /locus_tag="ACGI6B_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HFG9751239.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            1744..2460
                     /locus_tag="ACGI6B_001794"
     CDS             1744..2460
                     /locus_tag="ACGI6B_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFG9751240.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            2539..3849
                     /locus_tag="ACGI6B_001795"
     CDS             2539..3849
                     /locus_tag="ACGI6B_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFG9751241.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            3949..4485
                     /gene="lepB"
                     /locus_tag="ACGI6B_001796"
     CDS             3949..4485
                     /gene="lepB"
                     /locus_tag="ACGI6B_001796"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9751242.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            4651..5187
                     /gene="lepB"
                     /locus_tag="ACGI6B_001797"
     CDS             4651..5187
                     /gene="lepB"
                     /locus_tag="ACGI6B_001797"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9751243.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            5197..5952
                     /locus_tag="ACGI6B_001798"
     CDS             5197..5952
                     /locus_tag="ACGI6B_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFG9751244.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            6026..7693
                     /locus_tag="ACGI6B_001799"
     CDS             6026..7693
                     /locus_tag="ACGI6B_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HFG9751245.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            8652..9581
                     /gene="glsA"
                     /locus_tag="ACGI6B_001800"
     CDS             8652..9581
                     /gene="glsA"
                     /locus_tag="ACGI6B_001800"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFG9751246.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            9679..10242
                     /locus_tag="ACGI6B_001801"
     CDS             9679..10242
                     /locus_tag="ACGI6B_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9751247.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            10730..11566
                     /locus_tag="ACGI6B_001802"
     CDS             10730..11566
                     /locus_tag="ACGI6B_001802"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFG9751248.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            11850..12845
                     /locus_tag="ACGI6B_001803"
     CDS             11850..12845
                     /locus_tag="ACGI6B_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9751249.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13102..13506)
                     /locus_tag="ACGI6B_001804"
     CDS             complement(13102..13506)
                     /locus_tag="ACGI6B_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9751250.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            14528..15442
                     /gene="queG"
                     /locus_tag="ACGI6B_001805"
     CDS             14528..15442
                     /gene="queG"
                     /locus_tag="ACGI6B_001805"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFG9751251.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            15548..17785
                     /locus_tag="ACGI6B_001806"
     CDS             15548..17785
                     /locus_tag="ACGI6B_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9751252.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            17983..19152
                     /gene="nth"
                     /locus_tag="ACGI6B_001807"
     CDS             17983..19152
                     /gene="nth"
                     /locus_tag="ACGI6B_001807"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFG9751253.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            19346..19816
                     /gene="trmL"
                     /locus_tag="ACGI6B_001808"
     CDS             19346..19816
                     /gene="trmL"
                     /locus_tag="ACGI6B_001808"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFG9751254.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            19852..20700
                     /locus_tag="ACGI6B_001809"
     CDS             19852..20700
                     /locus_tag="ACGI6B_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9751255.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            20988..21992
                     /locus_tag="ACGI6B_001810"
     CDS             20988..21992
                     /locus_tag="ACGI6B_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9751256.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            22118..23230
                     /locus_tag="ACGI6B_001811"
     CDS             22118..23230
                     /locus_tag="ACGI6B_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9751257.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            23720..24481
                     /locus_tag="ACGI6B_001812"
     CDS             23720..24481
                     /locus_tag="ACGI6B_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HFG9751258.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            24484..25302
                     /locus_tag="ACGI6B_001813"
     CDS             24484..25302
                     /locus_tag="ACGI6B_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HFG9751259.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            25482..26084
                     /locus_tag="ACGI6B_001814"
     CDS             25482..26084
                     /locus_tag="ACGI6B_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HFG9751260.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    26696..26934
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            26998..28917
                     /gene="thrS"
                     /locus_tag="ACGI6B_001815"
     CDS             26998..28917
                     /gene="thrS"
                     /locus_tag="ACGI6B_001815"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFG9751261.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            29685..30776
                     /locus_tag="ACGI6B_001816"
     CDS             29685..30776
                     /locus_tag="ACGI6B_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9751262.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            31446..32786
                     /locus_tag="ACGI6B_001817"
     CDS             31446..32786
                     /locus_tag="ACGI6B_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9751263.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
     gene            complement(33364..33933)
                     /locus_tag="ACGI6B_001818"
     CDS             complement(33364..33933)
                     /locus_tag="ACGI6B_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9751264.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(34094..35344)
                     /locus_tag="ACGI6B_001819"
     CDS             complement(34094..35344)
                     /locus_tag="ACGI6B_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9751265.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            complement(35635..>36328)
                     /locus_tag="ACGI6B_001820"
     CDS             complement(35635..>36328)
                     /locus_tag="ACGI6B_001820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9751266.1"
                     /translation="YYKHKSLDKFISRQYYISMTQNIFKVIYIIYRQKGLIKMAKSFT
                     EIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGIAKGSFYTFFDSKEKLFFEVL
                     NGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKNPFLYSPNNPDFISLINKLPQ
                     DMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNALLSSLLVKENIEYNYIEVFD
                     FMVDNLVEHILE"
BASE COUNT        14366 a         3543 c         6427 g        11992 t
ORIGIN      
        1 ataatatttt aaagaaatct aagttgctaa gcactgtttt taagaagctt aaaatccccc
       61 atttaaagtt ttaagattct aaaaaaacaa gttataacta aaaaatggct gttaagacta
      121 caatacttat aagtaatact tatggtatat attagttcat aacagccact tttattttaa
      181 attaataatt ttattcaata atttgagttt atgttctaaa tcaagcttta aattaaagga
      241 tttaaagaag ctattctagt tgtagctaca taaactctgg atatttcata ccatgtttta
      301 aaatctacta ctccgctttg aggtaaacca aagattcctt ggaatatctt aacagcgttt
      361 tctgtatctg ttccatagat accatcttct ataacttttg gtacagctgg ataagaatta
      421 gatatagcat ttaactgatt ttgtatggtt ctaacatact gcccagaaga accaacttgt
      481 agtgtagtac cagggaatga cactggtaca ccacttacta taggtgctct ttcaaatact
      541 atctcatctc catagaaata tcttagtata ctctcatagt catatccttg gtcacctaag
      601 tctttactgc cccattgagt catttggtca ggacattggg atttttgtcc atcacaatat
      661 tgtgctaata gtggctgtct tgatgtagga ggtcttttta taaaagtatt aaaaatagca
      721 tctacaacta cgttaattgg ttcaaataag tttctattat ttataaactt atggtcgtat
      781 gctgttgttg aagttattgt aaagttatat cctttatttc tataccattc agtaaagact
      841 ctatttaaag taaatgatat tatagctatt acatttgcat agattgtctg ttcaggccaa
      901 gttgaatata tttcagatga cgctatgttt ttaatgtact ctttaaatgg aatccaatag
      961 tttggagctg atgaatcttc tggcaaacca tcatgaacta ctataaattc aggcactacg
     1021 gggttatcta atacaacgaa accagtagga ggtggtaatg gttttaagtt actttctgga
     1081 atcttaggag gataagtacc atatagagta tgttctccta tatcaaaaat caactcactt
     1141 tgtcttgaga aagaacgctt acttcttgtt ctaggagaca tgggaactcc ttgtcttgct
     1201 tcaattgtag ctaacaactg tgttccttct atgacaactg tctcatatcc atctgctatg
     1261 gcttccacta tatactggct ataaggtcta acatcagatg gttgtagaga gtaatctaca
     1321 tctggtgctg gtaatactaa accagtcact tgaccagatt cattacttct taggttttgg
     1381 tataaagttg atgagctttg gctaccatta gacattgaat atatgtttac tactgcattt
     1441 tgaataggtc tattgttagt agcatcaatt atgcttactg ttaagaaacc atcttgcatc
     1501 aaatcaccct ttctttaaat gaatttagtt cccttatatt tcaagatatg aatatagctt
     1561 gaaatttgtt aaataatata gatataaatt taaatttttt tgagtatttg agtatatttg
     1621 tatttaaata atttaaaata atttaatgta tattttagta tgtatttaaa acacgaataa
     1681 gttaaaaata agtagatgat ataaatcata aataaatcaa aaattttgaa aggaaaataa
     1741 tctatgaagc tagatttatt tttattagca ataattccta tattgatagg gatgttttgg
     1801 ataaggtcta aagatagata ttgtagagaa cccctaatcc atctaattaa atttttttta
     1861 ataggtgctt ttctaagtgt tataattata cttttagaaa atttactgat gaagtttaat
     1921 gtatttgaag ggtattcaga actaatatat gtatcatttg tagttgcagg acttgtagaa
     1981 gaaggtgtaa aagctctgat actgattcct gccctaatta aagaaaaaca ctttactgaa
     2041 aagttagatg gtattattta ctctgtattt ttagctttag gatttgcaac aattgaaaat
     2101 atggtttata ttttttctga aagtagaaat ctagcactgc aagttggtat taatagggca
     2161 gttatctcta taccagctca tgtaatgttt gcaattacta tgggctacta tatttctaag
     2221 tataaatttg agggaaataa aaataaaaga cgtgagtatt tgtttatggc ggtgttaatt
     2281 ccaattttat tacatggagt ttttgatttc atattgatga tagaatatag atgggctatt
     2341 atattactta tagtttatgt aataattttg tggaagataa atcttgataa acttgagaaa
     2401 tatatgaatc actctaaaaa agtgtttttc ggtaatctta ggaaaaagaa gaagaaataa
     2461 aatttaaagg aaagacataa ataattaaag tataataaat gacacacaag taaagcagat
     2521 tgaaggggga ttatagattt gacagaatta ccaaaggaat ttttagaaga tattgaagaa
     2581 atactaaaag atgaatatga tgattttata aaaagttatg aggaaagtaa gactactggg
     2641 cttagaataa atactttaaa aattgacaag aaaaaattat ttgatttgga gttgtttgat
     2701 ttgaatcaaa taccatgggc agaagaaggt ttttattatg atgataaaat tgacagacct
     2761 ggtagaaatc cactgcatga ggctggagca tactatttgc aagagccttc tgcaatgagt
     2821 gtaataccaa aggcagatat taaagagggt gaaaaagttc tcgatatgtg tgctgctcct
     2881 ggtggaaaat ctacatatat actatctaag ctaaacgata caggtttatt ggtttctaat
     2941 gaaattaatg ctagtagaat taaggctcta ggggaaaatt tagagcgatt tggagcgaga
     3001 aattgtatta taacaaacac cgattctaat agcttaagaa agacgtttaa tgggtatttt
     3061 gataaggtta ttatagatgc accttgttct gggcaaggta tgtttaggaa ggatgaggtt
     3121 gccatatcag attggagtta tgcaaaggta gtagaatgtc aatccatcca aaaagagatt
     3181 ataagagatg gatataaaat gttaaagaag gatggcattt taatttactc aacttgtact
     3241 ttttcaaggg aggaaaatga gcatgttata aatgagttta tcagtgaata tccacatgca
     3301 gaacttattg aaatggaaag attatggcct cataagataa agggagaagg tcattttgtt
     3361 gccaaaataa aaaaattaga agaagaagaa tgtaatgtca aagaattaaa aataaaaaat
     3421 ttagataaag aaataaagga ttatagggag tttgaaaaga aatttttaaa ggttgatttt
     3481 tatagtaata aaaaaaataa attttattta agaggagaaa atttgtacct acttcctgaa
     3541 atatatccag atacgaaaaa actaaaagta ttgagatatg gtttgcattt aggggtttta
     3601 aagaagaata gatttgagcc ttctcatgca ctatctcatt acttaaaagt tgaagatgta
     3661 aaaaatgttg aaaacttcag tgtacagagt gaatcaattc ttaaatattt aaaaggggat
     3721 gttgtaaatt ctaatgatag tagaggatgg gttttggttt cggttgaagg aataccttta
     3781 ggttggggaa aagaatcttc tggtgtaatt aagaatcatt atcctaaagg tcttagaaaa
     3841 gtattttaaa ctattagaaa aaagaagtat aatatttata ttgtaggttt atggtagatt
     3901 attttgctgt agattgactt aatatttaac acagaggaga cgattttagt gggtgaagca
     3961 gttaaaaaag aagttgtaga atggataaaa gtgattgtca tagctcttgt tttggcattt
     4021 gcaataactc gttttatagt gccaacaata gtcaaaggag aatcaatgta tcctacatta
     4081 gttgaacgtg attatttgat agttaacaga attgcgtaca aggtaggaga gccaaaatac
     4141 aaagatataa tagtattcaa aaccgactta acagaggaaa atggaaagaa aaaagattta
     4201 gtaaaaagag ttatcggggt tcctggtgac catgtaaaaa tacaagactc caaggtatat
     4261 gtaaatgata agttgttaga tgagacttcc tatatacata ataatcgtac tgatggagat
     4321 attgatatcg tagttccaga aggaaaatta tttgcaatgg gagataatag agaaaaaagt
     4381 ttagatagta gatacgatga ggttggattg gtcgacgagc ataccatttt aggaaaggtt
     4441 ctagtcagat tgtatccatt ttctaagata ggaactattg actaaacata taaaatttgg
     4501 agcaatcgct gattagctga aatcaccaga ccatacacaa tagttgtcta tataaaaaag
     4561 acaacacctt atttagtaga cttatctaaa agatagtgtt taagagacat tatcaataaa
     4621 gataatataa ctataaagga gaatttgtga atgaatgaaa ctattaaaga agagattgta
     4681 gagtggataa aaataattat tactgcactt ttttttgcat ttattataac tcgttttata
     4741 aaaccaacat tagtaaatgg agaatcaatg tacccaacac ttaaatcaca tgattatttg
     4801 gtagcaaaca ggatgacata taagttatca gaaccaaaat gtggagatat aatgatattt
     4861 aagactgatt tattacaaga gaatggaagg aaaaaagagc ttgtaaaaag ggttataggt
     4921 gttcctggtg accatctaaa aattaaggat tctaaggttt atataaatgg taagttatta
     4981 aatgaagttt catatataca tgataattat actgaaggcg atattgatat ggtgattcct
     5041 aagggaaaag tatttgcgat gggagacaat agagaagtta gtttagacag tagatataaa
     5101 gaagtgggat tagtagatga agaaaatatt aaaggaaaag ttattttaag agtatttcct
     5161 tttacagata taggtatttt tgagtaggtg atttgtatgg cagttggtat agcgtcctta
     5221 ttgtttaata ttgaggaggc tttaaacatt tgtgagagta taaagcaaat aactcattta
     5281 gagattggaa ttgacaatat atctgaatgt agtgagttat gtaaatataa agagagaatt
     5341 tctaagttag gattatctat agggatacat ttgcctatgg aattaaatac ttgcgaaaac
     5401 atagagtata tacgaaattc atggataaat tttattgaga agatagaatt tgaactaaaa
     5461 ggatttgatt taaggtattt taaccttcat ttgggatatg ttatgacaaa tagagttaga
     5521 aagaatagag acaaatattt aggaaatagt gtggattttt tagataaatt aaatacaaat
     5581 tcatatgtat gtatagagaa tacttattca aaaggtgggg atttttcaaa tattggaaat
     5641 atatcttatg attttgaata tatatttaag aggattaaaa attctaagat ttgtttttgt
     5701 tacgatactg gacactgttt aattgatgag gatgcttatg taaagaactt aaaggataaa
     5761 ataagactga tacacttaag tgataatgat ggtatcaatg atacacatgt tggtatagga
     5821 agaggaattt tatctgaaga ggggattaaa gagatcttaa cattagatgc agaatattta
     5881 gtattagaaa taaattacga agacattgaa gatactataa gtaaattgaa taatattgta
     5941 ggagagggtt aaatacctct ctttatcttg ttgatatgcc tcattaatta aaatttatat
     6001 tcatcaaaag gaaggagatg ttgaagtgaa aaaaataaac ttaaaaatag aaggaaaaga
     6061 taaggaatat tcattagaag aaaattcccc tggaatcaga ttgggagata tagcaaaaga
     6121 attttgtgat gagcataagg gttatataac acttgcagtt gtagacaata aactaaaaga
     6181 gctaaattgt agagttaaga aggattgtga gataaatttt ttagatacta ctaatgaaga
     6241 tggagagaga gtttacttta gagtcatgtc atttatattt gtaatggcat gtagagaaat
     6301 cttttgggat agcagagtta caatagaaca ttcactatca gatgggctat attgtgaagt
     6361 tcatatagat agaaagctta aagaagcaga tgttgaaaat ataaaaaaca agatgaaaga
     6421 gatagtaaac aacgattatg ttatagaaaa aattgaagtt acaagaaatg aagctatttc
     6481 tatctttgaa aataatgaaa tgtatgaaaa ggcagagctt ttgaaataca aagagtatga
     6541 tagtgctaaa gtatataagt gtagaaacta tatagaccat ttttatggtt atatgttgcc
     6601 atcaactggt tatataaaat catttgatat taagctttat aatggaggcg ttataatttt
     6661 aggacctagt gaagaagata agactcttcc tatgaaattt gtacctcaac ctaagctatc
     6721 aagtgtatat tatgaagcag aagaatggtc aagacttatg ggaattgaca aggtagtatc
     6781 tcttaataaa ataatagaaa acaatgaata tggagatata ataaggacat ttgaagcatt
     6841 gcatgaaaag aagttatccc aaatagcgga tatgataaag gatggcaata agagagtagt
     6901 tttaatagca gctccatctt catctggaaa aacaagtttt gctcacagat tatcaataca
     6961 tcttagagta aataatttaa atcctatttc aatttcttta gatgattatt ttatagatag
     7021 aaaacatact cctttagatg aatatggaaa ttatgatttt gaatcaattt atgcaataga
     7081 tttagagaag tttaacttag acttaaagaa acttttagct ggtgaagaaa ttgatgatat
     7141 aagattcaat tttaaagaag gtaagagaga atacactggt aaaaagataa agatagattc
     7201 aaatcagcca attatattag aaggtataca tgggctaaat cctattttaa catcatctat
     7261 accagatgaa gataaattta agatatacat aagtgccctt actcaaataa atttggatga
     7321 ccataatcga atccctactg ctgatttgag aatgataaga agaattgtta gagactataa
     7381 ttttagagga tatagtgctg ataatactat tttacaatgg gcatcagtta gaagaggaga
     7441 aaagaagaat atattcccgt accaagagga ggcagatgct atatttaact ctgcttgtgt
     7501 atatgaattg gcagttttaa aacgatttgc gaaacctttg ttggaagaga taaaggaaga
     7561 taatcctgca catattgaag ctactagatt actaaagttt ttgcagtatt ttgttgaatt
     7621 ggacgatact tctgacatac caggtatttc aattcttagg gaatttattg gaggaagtaa
     7681 aatagttgat taatgtgtgt attatagatt gttttatgaa gattaaatgg catagcaagt
     7741 agtgttattg gtttatgtta agtatattaa gtgtaaagtg gagaattaaa ttgatacaga
     7801 ttttaaagtt tgagttgata tataaaaagg cttgttttat acttttgtat gaagcaagct
     7861 ttttatttta aattttactt ttttgtttaa tattttgtta atttatgtga ttatttttta
     7921 aaagagtaaa agagtaaaag agtaaaagag taaaagagta aaagagtaaa agagtaaaag
     7981 agtaaaaggt gttgggttag aaatttttgg tgttataaaa atttaaatta ctggattatt
     8041 agattgctgg attgctaaat tactaggttg ttaaattatt gaattattag gttgttgaat
     8101 tactaaatta ctaggttgtt aagttactgg attatttaga tagttagatt actaggttgt
     8161 taaattattg aattattagg ttgttgaatt actaaattac taggttgtta agttactgga
     8221 ttatttagat agttagatta ctaggttgtt aaattattga attattagat tgttgaatta
     8281 ctaaattact aggttgttaa gttactggat tattagatag ttagattact aggttgttaa
     8341 attgttaaat tattgaattg ttagattgtt gaattactaa attactaggt tgttaagtta
     8401 ctggattatt agatagttag attattagat tgctaaatta ttaggttgtt aaattactgg
     8461 attattagat tactaaattg ctaggttgtt aaattattaa attattaagc tatttaaact
     8521 atttaaattg tttgaaaaat gaattgattc ttgtagaaaa taattttaaa gtataatttt
     8581 ttttaacatt gttttattat agtataaact tgtgctaaaa tagaatatat aacataatat
     8641 aagaggtgca tatgaatacg ttagatgaga cattattaaa agaaattata agttctaata
     8701 agaagtatac taattatgga caagtcgcta gttacatacc agaattaaaa aacgctagaa
     8761 gaaatgattt aggaatttgt atcatagata gcgaaaataa tctttatagt gctggaaact
     8821 gtagcacaaa gtttactata caaagtattt caaaaccaat tgtgcttgct atggcactta
     8881 tggataatga ttgggaagac gttttttcaa acgttggaat ggagccaagt ggggacccat
     8941 ttaattcaat aatgaaactt gaaattaatg acacaaaaaa accctgtaat ccaatgataa
     9001 atgcaggggc tatagtaacc acttccctta taaatgggag ttgtttagaa gaaaaagaag
     9061 aaagaatgct tagtttcttt agaaagttag caaaaaatga taatatagga ataaactatg
     9121 atgtctataa gtctgaaaaa atgactggag atagaaacag agctatggct tacttattga
     9181 aaagtgatgg atttatcaga gggaatgtag aagatgttct agacctttat tttaaacaat
     9241 gctcaattga gatagattct gtagatttag cgagaattgg aataaatctt gcaaattatg
     9301 gagtagatat agaaaatggt gagcatttaa tgagtgaaat ggtttctagg attgtcaaga
     9361 catttatgat gacttgtgga atgtatgatg cttctgggga atttgctatt aaagttggca
     9421 ttcctgcaaa atctggagtt gggggtggta taatggcttc tgtgccggga cgcatgggga
     9481 ttggtgttta tggacctgcg cttgacaaaa aaggtaacag cgtcgcagga gttaaagttt
     9541 tagaggagtt gtcaaataaa ctcaaattaa atatttttta aaatattaag aaaggatttt
     9601 tttgacaaca acatataatt taatatataa ttaaattgta aagaaatatg aattaaataa
     9661 attttttagg aggggtcaat gaaagaggta ttggtattac gagatttgga gtgcataaag
     9721 gctattgcgc atccacgtag aatagatatt ctaaaagcat ttgacaaatc gccattgtca
     9781 gcaaaacaat tatctcaatt attggaagaa cctcatgcaa aaataaacta ccatataaaa
     9841 atgttataca aagttggtat tttagaatta gttgaggaaa aaataaagtc ggggattgtg
     9901 gaaaagtact attatccaag tgcaaaaaat gtggttatag ggaataggat acttaatttt
     9961 tctttagaca atggggaaga aaaagaagaa ttgtacatat caaaatttga aaatatgagt
    10021 gaagtttttt ataaggcagc agaggaagat gttttagaaa atgaaaatat agttgattat
    10081 catgatattt ctcttactca tgatgaatta gttgaactta gtgacactat gaagtcaaag
    10141 atagatgaaa ttttaaataa aagacaacac aatgttgaag gttctaagta tgatatagct
    10201 atggttatag ttcctactct tgaggaggaa tgtcctagtt agagaattaa ttatggatta
    10261 attaaagata taatattaga gattgaacat caaaatttac ttaataatca aaaaatagtt
    10321 tttggtatgg gtaaaacaat gatttttaat ctctattttt ttgttttttt atacctattg
    10381 tatttgtaaa taatatgtat agtgtaagat gtatacacta tctaagaagt gcttagataa
    10441 tatacaaagt atgtaagatt ataatatttg aaattgatta aaatgggtat aattaaagtg
    10501 taaatattaa aattaaaaat taatataaat taaatcaatg ctagaaatat gttaaaagag
    10561 gaatgagatt agttgggata gaacatccct tagagcttac gaatttttag tgaaaaagta
    10621 ctatcaaata ggaatctcca tcttcatatg ttggctaaat gtagttaagg tggagtagtt
    10681 caaaattaaa attgagtaca agtagtagta caaataaaag gagattaata tgattaaatt
    10741 aggaacacat ctttcaatag caaaaggatt tgctaatgcg gctgagacag ctgtatatat
    10801 tggtgcaaat acatttcaat tttttagtag aaatccaaga ggaggaaatg ctaaagaatt
    10861 tgatgaaaaa gatataatta aatttcaaga aattagaaaa gaaaataatt ttgcaccact
    10921 gcttgcacat gcaccatata ctatgaatct gggtggtaca aaggatgatg tatacgaatt
    10981 tgcaacaagg gtgatagatg aggatataag aaggatggac tcattaggaa ttgaatacat
    11041 gtgttttcat cctggaagtc atgtaggtgg tggtgtagat tttggcatag atagaatagt
    11101 aaaaggattg aacaatgcca taaagggtga tgaaaatata actatacttc ttgaaactat
    11161 gtcgggaaag ggaactgaaa taggttttaa atttgagcaa ttaaaaagta taatagacaa
    11221 aattgagcat aaagaaaaaa taggtgtgtg tcttgataca tgtcatattt tttcagctgg
    11281 ttatgatata gtgaatgatt tagatggggt tttggaagaa tttgataagg ttataggcat
    11341 agaaagattg aaaactgtcc atctaaatga tagtatgatg ccatttggag acaaaaaaga
    11401 cagacatgct cctatagggg aaggaaagat aggattaaaa gcattaattg attttatgga
    11461 acatcctagt ctaaaacatt taccattttt cttagaaact ccttttgatg atgaaggtca
    11521 caagagagag cttaaaatga taaaagaaat actatctagt aaataaacct ttattaaaaa
    11581 tatgtgaaat actccaccct caacataatt agggtggaga tacaatcatg aaatgtgata
    11641 atttaaatta atggatagga ataacgaaaa ttgtatacaa attaactggg tataaagtat
    11701 gattatatta tgaatagact gatataaaat ataattatat tatgaatagt atgtaaatac
    11761 attgcaaaga ttctcataat ttgttgtata attaaaggtg acatatttat attatataaa
    11821 aaattattaa aagttaggtg gggaaagata tgagaaattt aggcaatact aacatgaaaa
    11881 taaagagagt tggctttggg ggcattccaa tacaaaggat tactcaagat gatactaact
    11941 tagttataaa tgagttagaa aaacaaggaa taaactttat tgacagtgca agagggtata
    12001 caataagtga ggaagcaatc ggaattgcca ttgaaggaaa aagagataaa tttttcttag
    12061 ctactaaaag tatgtccaga gattatgatt caatgaagag agatgttgaa ataagtttaa
    12121 ataattttaa aactgatttt atagatttat atcaattcca taatgttaaa gaagaggagt
    12181 atgataatct ttttaaagat aaaatggctt atagtgccct tttagaggca aaggagcaag
    12241 gtaagataaa acatattggt ataactagtc acaatttaaa tactatagaa aaagctatag
    12301 aagatggaaa atttgacact atacaattcc cttataatat agttgaagga caagcagatg
    12361 aagtgtttaa aaaagctcat gaaaaaggaa ttgggataat agtaatgaaa cctttagctg
    12421 gaggtgcttt agacaatgca acactggcta ttaaatatat actatctaaa gagtacatag
    12481 atgtagttat accaggtatg gagagtgttg aacaggttag acaaaatgtc gctgtacttg
    12541 aaaatcttgt gctagatgaa aaagataata aggaaattga agaaataaga aattctttag
    12601 gtaaaaaatt ctgtagaaga tgcgaatatt gtatgccttg tgctgtagga ataaatatac
    12661 cattgagttt tttatgtgaa ggttattata ctagatatgg attaaaagag tgggctaaag
    12721 aaaaatatga ggttatggat gttaaaccta ctgagtgtat tgactgtggg ctttgcgaga
    12781 gtagatgtcc atatgaactt cctataagag aaatgcttaa gactgttgtt gaaaagctag
    12841 gatagatttt atttattgaa atcggtgtat taaatttact ccgatttctt tttattagat
    12901 gtggtcttat atacaaatat taaatttaca tagttatagc tattatataa atttaatatt
    12961 tgtttaatac ttattaaata tttacttaat gctttttaaa aacatttgtt tgagtataga
    13021 cataacacaa ttattattgt tgtattagta tagtttcaaa tactggagta tcaatataat
    13081 gtcgaataac acataaaatc tttatttttc aaaaatatta taggatatat cgtatttaga
    13141 tactggataa agttcaattt caaccctagg gttatcttta tctataaaat caaataaaag
    13201 tactggctta agttgtttat cattttttat tattccactt ttttcaatac catcacaaat
    13261 actttttgga taattatgta aatcccccat tctcttattt gggaagaata atttcaagac
    13321 tgttattaga ttttcttcga cagtttcccc ttgatactgt tggtttataa aacctgcaat
    13381 catgttttcg tatgcaagat acttagaata ttttcccttt gaaggcatcc atgcttgacc
    13441 atttatgtta tgaagcttaa aattagattt actaataggt ctacctggaa ttgttatttt
    13501 aatcaaattt ttcaccatcc ttatacagta tttattataa tctgtatatg atttaaaagc
    13561 aaattgttat ttaaagtgtt tttacagagc ttttttgtaa aattttccac tttagatgta
    13621 gtaatataag ataagtattt tgcttacaaa ttttaaaatt tgatatatga tggacatatt
    13681 catattagtc tattataaga ccttcataat attaataagt atagtttagt aaagagagta
    13741 aaaagaaatc catataagtc aagtaaagat ttaataaatg gcaatgtaaa tgggatatta
    13801 attataagaa aaaatcaatc tttttgctta actatagaaa gaatgtcaaa atataactaa
    13861 tgatatttga agttttagag tttatattaa aaggtaagaa ggttaagtgg gtaaaaaagg
    13921 ttgaatagat taaaaaggct aagtatctaa aagagttaag taggtaaaaa agttaaataa
    13981 gtaaatgaaa aaggctaagt atctaaaaga gttagtagtt aaataagtaa atgaaaaagg
    14041 ctaagtatat aaaggagtta gtagataaaa aaattaaata gataaaaaga ttaagtatct
    14101 aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt caaaagagtt
    14161 agtaggtaaa ataattaaat agataaaaag actaattatt taaaggagtt aagtagataa
    14221 aaaagttaaa taagtaaatg aaaaagacga agtatctaaa agagttagta aataaaaaag
    14281 atgaagtatc taaaagagtt agtaggtaaa aaactaaata gataaaaaga ttaagtatct
    14341 aaaagcgtta gtaggtaaaa aattaaatag gtaaaaagac gaagtatcta aaagatttaa
    14401 gtaggtaaga aaattgaata gctaaaaaga ttaagtattt aaaagagtta gtagctagaa
    14461 aaggttaaaa agactaaaaa aattaaataa atttaaaaag tcaattagat taaaaaggaa
    14521 taattttatg gataataagg aattaaaagc attttgtaat ttaataggat taaactgtgt
    14581 aggagtagca ggggtagata agtatgataa tttagaaaag atacttaagg acagacaaca
    14641 aaaaggttat ttaactggta tggaagaacc aataattgag aacagaatta acccaagaca
    14701 tataatgaag gatgctaact ctataatagt ctgtgcattt ccatatcata taaatagaca
    14761 agaaaagaat aaaaattcaa atttatcaaa atattgtcat ggaaaggatt atcacattgt
    14821 agtaaaggac tttttacaac aaatttgtga ttatatttct aaaacagtaa cagattttaa
    14881 atacaagtta tttgctgata atggaccatt agttgacagg tatttagctt atttatcagg
    14941 aataggatat tttggaataa ataataatat cataactgat gaatatggtt cttacgtatt
    15001 tatagggtat atagtaaata attataaatt tgaagaagat aaaccactag aaaaaagttg
    15061 ttttaaatgt ggaaagtgtg taaaatattg tccgggaaat gctatattgg gaaattatga
    15121 tatgaacccc aaaaaatgtt tatcatatat aactcaaaaa aaaggagatt tgtcagagaa
    15181 agaaaaagtt gttttaaaga atggcaaaaa ggtatttgga tgcgatattt gtcaagaagt
    15241 atgtccacat aatgttgaaa taccaacaac tcatattttg gaatttaaag aaaatataat
    15301 agattatttg aattatgatg agatacaaaa tatttcaaat aaagaattta aaaggaaata
    15361 tggagataga gcttttagct ggagaggaag aaatataata aagagaaata tggaaataat
    15421 tttagataag tatgatgact aataataaga gtctgtttca tatactttaa tgtgtattta
    15481 ctttaacact tgttgtgtaa aagtcatatt atatcattaa agttaaatat ggggaggtgg
    15541 gggaatattg aaatataata atacatctga aataaatcca ctagattata tattgggtca
    15601 agaaagagct gtagaagcta tggaattagg gttgaaaata aataatccag cgtacaacat
    15661 atatatagct ggtgaacctg gaactggtaa aagtacttat gccttaaagg ttttaaatga
    15721 gtatgcatcc aaaaaaaata cgcataaaga ttggtgttat atatataatt ttgaaaaccc
    15781 tagagagcct attatagttg aattagaaaa ggggtttgga agagaactta agaaagatat
    15841 ggagaaatta attgaaagtc ttttagaaga ttttaaaaat gcgtttgaaa gtgaaaattt
    15901 tgaaatagaa aaaaataaac ttctggatga atatgaaata gaaaaagata tgcttttgaa
    15961 gcaaattaaa aaatatggag aagaaaaagg atttaaatta aaacagagta aaatgggaat
    16021 agtttttatc cctttgaaag aagaggaaga tgaaagtgat gagtctgatg aagagtttta
    16081 caaagctaaa agagaactag aaaatatggc gatacaggtc gtatataaaa ttagaaactt
    16141 agaagaaata gctgaaaaag cagtgttaga gcttgaagaa gagatagcta aacttgtagt
    16201 agaacctcat atcaagagat tgtgtgaaaa atatgaaaat tatgataaga ttcaaactta
    16261 tctggaaaat ataaagaaag atataataga atatatgtat ttattctatt tagatgaaga
    16321 ggagttaaaa gataagtatg ataaagagca ttttattaaa tataaaatta acctatttgt
    16381 agacaatgga agctcaaaga ataaagaatc agcacctgtt gtagtagaga taaacccaag
    16441 tccagcaaat ctatttggaa aggcagagta tgattatgca aatggaaata taaagactga
    16501 tttcacaaaa ctattatcag gagcatttca tagggctaat ggtggttatt tagtacttta
    16561 tgcagaccaa ttactaaaat atacaatgtc ttgggaaatt cttaaaaaga ctttgcagac
    16621 taaaaaagta atattggaaa cacaaacagc aattaaacca gaaaatatgc cattggatgt
    16681 taaattagta ctgattggaa gccactatat atatgatatt ttatatagat atgatgaaga
    16741 ttttgaaaaa tattttaaag tatttgtaga ctttgatagt gaaatggata aaaatgaaga
    16801 taatgaagaa ggaatagcaa gatttatagc ataccaatgt gataagaata atttaaggca
    16861 ttttactaaa tcagcagttg aagaggttat taaatttagc actagattat ctggtgattt
    16921 agaaaaatta tcaactaagt ttaataaaat aatggaagtt gtaatagaag gaagtgctta
    16981 tgcagagttt agaaattcgg agtatacaga gcagtgtgat gttaaaaaag caataagtga
    17041 aaagagaaaa agaatcaata gagtggaaac acatatggat gaatcaattg aaaatggatt
    17101 tactctaata gaaacagagg gaagaaaagt aggagtgata aatggtttgt cagtcttaag
    17161 tacaggagaa tactcttttg gtagagcttc aaggattaca gcgaccacta gcccaggaag
    17221 taaaggcata gtaaatatag aaagagaagt aaatatgagt ggctctatac ataacaaagg
    17281 agtacttata ttaggtggct atctttctga gaattttgca caggatttac tattgtcact
    17341 aaatgcatat gtttgttttg aacaaaatta tggtggaata gatggagata gtgcatcttc
    17401 tgcagaatta tatgtactat tatcatctct tagtggagta tcaattaaac aaaatattgc
    17461 tgttacaggt tctataaatc aaaagggtga tatacaagta gttggtggta taagtgaaaa
    17521 gatagaagga ttttattcta tatgtaaaac taaagggtta aatggtgagc aaggcgtcat
    17581 aattcctaga aaaaatcaaa gaaatctcgt tttatcagat gaagttaatg atgcagtgcg
    17641 agatggaaaa tttaagatat atactgttga aagagtagaa gaggctatag agatattgac
    17701 agatgttaaa tttgaagaaa taaagaaatt ggtaaaagaa aaattaatat ctttcagcaa
    17761 gatacaaact gtgtcaaaag aataagttta ttatagaatt taatgggtat ttatgtataa
    17821 taaaaagcaa gaactttaat aggtttttgc tttttttgta aatatatata aattaaattc
    17881 ttattttgta tgttttatat attgatgttt atttttttgt tgatatgatt aaactattta
    17941 taaatagcgt tttataaatt ggtattttga agggggataa ttatgaaaaa tgtaaatcta
    18001 aaagagataa atgaaaaaga tgtaattttg gaagaagaga taaataaaaa aggaatggat
    18061 aagaaaaata taattgagaa ggaagagata aataaaaaag gaatagataa gaaagatata
    18121 attgagaagg aagagataaa taaaaaagga atagataaga aagatataat tgagaaggaa
    18181 gagataaata aaaaaggaat gaataagaaa gatataattg agaaggaaga gataaataaa
    18241 aaaggaatga ataagaaaga tataattgag aaggaagaga taaatgaaaa aggaatagat
    18301 aaaatgattg gtaaaaaaga tgagataatt gatgagaaag atatagagaa ttttatggat
    18361 attgaagaag ataaaaaaag tatcaaagta agtaaaaaaa gtactaaaaa aggtgaaacg
    18421 gagaaaaaaa caaagaagag tagttctaat aaggctacac aaaaaacaag taaagaaact
    18481 aaaagtaagt ctaaaattaa aaatgagtca gcaaatgaaa aagacgtaaa taagatttta
    18541 gacgagttag aaaaattgta tccagatgct aaatgtgagt taaattatgg gacagccttt
    18601 gaacttttaa tagcaactat tttatcagca caatgtactg atgtaagagt gaataaagtt
    18661 acttcagagt tgtttaaaaa gtacaacaca gctagagact ttgctaattt gagcattgaa
    18721 gaaattagta aagagataaa gagttgtggt ctttacaaaa gcaagtctca aaaaataaag
    18781 gatacatctg aacagctatg tgaattatat gatggtgaag tacctgatag cttagaaaag
    18841 cttataaaat tacctggtgt tggaagaaaa acggcagggg ttgtacttag taacgctttt
    18901 aatcatccag caatagctgt agatactcat gtatttagag ttagcaatag aataggaata
    18961 gtggacgagc caaatcctca aaaaacagaa tttgcactta tggaagctat accaaaagaa
    19021 agatggtcac actcacatca tgtattaatt tttcatggaa gaagaatgtg taaagctaga
    19081 aatccagaat gtgcaagttg cccaataaaa gaagattgta attattataa agaacttaat
    19141 gaaacgaaat aacttttaga aagtaagttg tacaagaggg attttctgat ataataatac
    19201 ttgtgaaaat gttagataaa aaactattat atacatgaaa taaaaattat tattaacatt
    19261 attttatata atagtataat attattgtta ttctgtttat aaaataaaat ttgataaata
    19321 aaagcgatac ttagggagga ttaaaatgtc attaaatata gtattagtag aaccagaaat
    19381 acctcaaaat acaggaaata taataagaac ctgtgcagca acaggttcaa ctttacattt
    19441 agtaaaacca ctgggttttt cattggatga taaacaccta aaaagatgtg ggcttgacta
    19501 ttgggatata gctgatatac aatattacga tagctttgaa gaattacaag aaaagtatcc
    19561 aaatagtaaa tacttttttt ctacaacaaa ggctaaacag tcacattcag atgtgaaata
    19621 tgaagaaaat tgttttatag tatttgggaa agaaacaaag ggattaccag aacctttgtt
    19681 gaaagcaaat atggatactt gtattagagt gcctatgttg gatgttgaaa aagctaggtc
    19741 attaaattta tcaaattcag ttgctattgt agtgtacgag gctttaagac aaataggata
    19801 cccaaatatg aggtagtttc aacaagccta aatgagaaag gagcatactt aatgaaaaat
    19861 ctaaagataa tctgtgacag tttatcggac gtgccaaaag atttgttaga taaatatgat
    19921 atagatatag tttcattaac agttatactg gaggaaagag aatacaagga tagaattgat
    19981 atatctggag atgaatttta taaaaggctt agagaagaaa atgtatatcc aaaaacttct
    20041 caagctactt atgcccaatt taaggaaatt tttgagaaat atacaaatga ggatagagag
    20101 atactttata tagcagggtc tgccacagct acaggtacgt atcaaagtgc tgtaatggca
    20161 aagaatgatg tagatggtaa aatatataca tatgactcta acttgctttg tatgggaact
    20221 ggtttacttg taataaaagc aggagaactt gcaagtgaag gaaagtctgt tgctgaaata
    20281 ttgcctgttt tggatgaatt aaaagagaag gcttttgtta tattttcagt agatacattg
    20341 gaatatttac aaaagggagg tagaatatca tctactaagg cagcaatagg aagtatatta
    20401 aatataaaac cgattcttga aataagagat ggattagttt cgcaaatatc tcaagtaaga
    20461 ggaaagaaga atgtaatttc taagatgatg gaattgataa aatcaaattg tggtgatgat
    20521 ttatcagacc aaattgtgta tataggatat agtgatgatt ttaaggaaaa agaacaatta
    20581 tctaaggtta tgcaagatga attaagacct aaagaaatta gatttttcca aattggtgtg
    20641 tgtataggtg cacatgctgg tcctggtgtt actggtatca tatgcttaaa aaataaataa
    20701 tatatttttg gatgacatta tttatttttt tattaaatta ggatagaata ttaattgttg
    20761 agatgttaaa ttggatttat tttggatgaa attaagtgtt gatatattag atttagtttg
    20821 tagggaagtg tgtaaataga taagaatcta ttgcacactt ttttatagta agatttatta
    20881 aaaaaatttc aaaatgtact tgtaaattgt atataaaaaa attataaata ggatgtaaaa
    20941 gtaaatagga tataaaaata tcgtaaatgt atgaggagtg aaatttaatg cgtttcaaaa
    21001 gaatattgat attgatgtta actgtagcaa tgatagctgg aatgttggtt gggtgtgcta
    21061 ataacccaag taataatcct agctctagca aagataataa aagtgatata agcgataaaa
    21121 gtaattcaaa aaaatctgta tcaatgatac tagatattga aggaacaaat aatgaagcta
    21181 tgaataattc agctttatta gcattaaaca atgctcaaaa gaaacttaat atagatacaa
    21241 acaaagtgga atcagatgat tcctcaactt tttctaatag tatagatatt ttatgtaatg
    21301 acaattacga tttaattata gctgtaggag ctagatttgc aaaaccattg gagatggtag
    21361 ccaagaaata tccaaagcaa caatttgcaa ttatagacta tgagtatgac aagcaacctt
    21421 ctaatataac ttcaatttct tatgaagata ataaatctgg atacctagct ggattaattg
    21481 ctggaaaaat gactgaaagc gataaggttg gttttatagg aggaacaaag agttctagta
    21541 gagataaatt tgaatcaggt tttagagagg gtgttaaatt ttctaatagt agtataaagg
    21601 atatttcagt tgagtatgca gatgttttta aagatagcaa atcagtagaa tctatagcta
    21661 aaaaaatgat ggataatgga gtggatatta ttttttctac tactgaagat gattcaaaag
    21721 ctgtaataga tgcagtaagg gcaaaaaata aaaaagttat agcaacgaat aaagaccaac
    21781 atgagttagc tccagaaaat gttataagtt ctattgtaaa agattttgag aatcctactt
    21841 ataatctgat acaaagtttt gtaaaaggaa attataaagg cggaaaggtt atagaaaaca
    21901 cagttaagtc tggaagtact ggacttgccc aaaatagttc tcaaaatatt cctccagatg
    21961 tgttagaata tgttaataag aacaataaat aatctactat aatatccaag aatacttatg
    22021 agtttagtta tgaggtgaat tggatgttat aatagacata tatagaaaga catatgcaga
    22081 ggtaggtcag acctatctaa atcagggagt gaattttgtg aaaataaaaa ggatattgat
    22141 ggcttgcttt gtagtagttt ttacaatttt tatattagct atatgtaatt ttgattttgg
    22201 gtttgataaa gtaaataaga ataattctaa tatagatagt gctaaatcga aacagagtga
    22261 gtctaaaagt ttgctaactt cagcagaaaa ggataaatca aatagtaaaa gtaagaataa
    22321 agaagatgac acagaagatg attcatatgt aagaaaaact attggagtat tgttagatac
    22381 cgaaaactta aataacgaat catataataa caatgtacta tcagccttaa aaagggctga
    22441 cgaagatttt gatgttagga taaaacttga aaaaccaaag acttctaaaa gttttaaaga
    22501 gagtatagaa aaactgcata agtatgaacc agattttata atagcagttg gagccagatt
    22561 tgcagagcca attaaagaag ctgctactaa atatcccaaa cagcaatttg caatagttga
    22621 ctatacttat aaagttcagc cagctaatgt aatttctgta tcatttcaag ataatgttac
    22681 tggatattta gcaggactta ttgcaggtaa aatgactgat actgggaaag tcggttttat
    22741 aggaggagtt gagggttcta gtagagataa gtatgaatct ggatttagaa aggggcttaa
    22801 aacttcaaat aaagatgctg agttactagt taagtattca aatatatttg aaaaccctga
    22861 atcagctaga actattgcaa aggaaatgag agattctgat agagatgttg tattttcagc
    22921 tacagaagat gatagtaaaa gagctattca ggtagcaaga gaaaatggtg gtaaagttat
    22981 agctattaat aaagaccaat acgatatggc tccagataat gtaataagtt caattataaa
    23041 agatgtagaa caacctgtat atgaactgat aaagaacgtt gcaaaagatg gttttaaggg
    23101 aagtaaagta atggaattta aaataaaaga tggggaactt ggcttgactt caaagagcag
    23161 tagaaatata ccaccagatg ttttggaata cataaaaaaa gagtataaca aagtgaaaga
    23221 tacatattaa ttattgatgt agaagattat aaaaatatat tgcttaagtt tagattagca
    23281 ataaaaagat atatttatta aatttgtgct aactgtacaa aatatattga ttaagtttag
    23341 attagtgata aaaagatata tttattaaat ttgtgttaac agtatggaat atactgctta
    23401 agtttagatt ggtgataaaa ggatatattt attaaattta gtctaactgt acaaaatata
    23461 ttgattgagt ttagattagc aataaaaaga tatatttatt aaatttgtgc taactgtacg
    23521 gaatatactg cttaagttta gattagtgat aaaaagatat atttattaaa tttgaaccaa
    23581 ctgtacaaag ctatactaat taagcttaga ataactatat aaaattataa caataagtgt
    23641 gtgggcagaa aaattctgct acacacttta tttatattca cataaaaaat ttatcaaaaa
    23701 aaagaaaaag gagtaaaaaa tggaatttac aagagaacca tttatactag atgacgattt
    23761 gtgtctagct ttggtggaca aaagaattag ttatctaatg gaaaatgaat taaaattaag
    23821 aaatatagag ataataaaaa caatagaatg taaagaatta tatgaaagca taaaatatca
    23881 cccggatata tgtatttgta atttaggaaa aggagatata attgttgcgc cgaatgtata
    23941 taatcaatac aaagagttat taagtagata taaatttaat ataattaagg gagaagctaa
    24001 tttattaaat agataccctt atgatatacc ttacaatatt gcgatagttg gtgactatgc
    24061 tatacacaat tttaagtata ctgacaaagc aatattagat tatattgaaa ataataaact
    24121 taaaaagata aatatagagc agggctattc taaatgctca atctgtatag tagatagaac
    24181 gtctataata acatcagata aaggtatttg gaaaagtatg aagaatacag aaatagagtg
    24241 tttattgata gaaaaaggtc acataaattt atttgagatg aattatggat ttataggtgg
    24301 atgtacaggg ttgatttcta aggataaact agctttttgt ggagatgtta aaaaacatcc
    24361 tgactatgaa cgtataaaaa gttttgtaga aagtaaaaac aaggagatag ttacacttag
    24421 ctgtgaaaat ttactagata taggttctat agttcctctc atgacaagaa aggagcgata
    24481 actttggttg agaaggataa tatttcttcc atgaaatttt tgaggaataa taatgtaaac
    24541 gatagattaa tatatacgaa tgacattgta gaagtagtga tagattctgc aaagcaagaa
    24601 gtgttaaata aaaaaatact taatggtact aaagctgaac aaagagcttt aaatattgat
    24661 gagatatcaa cggaaataac tagtatgata ataagtataa tgaaagagaa acttttgaaa
    24721 gaatacattc taaaatgcta tgggcgtaca tatcctgaag ataaaaataa tatatacata
    24781 tattcactga acatatttgc taaaaaagag atttttatga gagaaacagt atttactagg
    24841 gtgagaaatt atatagaaga aaatgatttt ataaatgtag aaggttttat tagatttaga
    24901 atgagagaat ttatgaaata tatttctgct ataggtgata ttgcagttga ggagtattta
    24961 ataaagaaag accaagatga atttataaga gttttaaaat actttataga tactcaagaa
    25021 gaaaagattg atttgcttaa agttcatata atggaagata atacatttgt tttatacgat
    25081 aaaaatggaa ataagataga cagtatagat gatgaagaaa ttataaatat ggtaataaga
    25141 gaaaatttaa attatgagga ctttttaata agtaccttat taactctatg tcccaaaaaa
    25201 atagaaatat tggatttgtt aaataataat tgctcaaagg aaattataga tactgtagaa
    25261 gcaatttttg aaaataaagt tagtataatt atggaaaact agaatctaag catataaaag
    25321 ctatccatat aaaattaaaa aaattctttt taaatcgaca caaaagactt ataattgtat
    25381 ataaagctat agtataacta gagttattaa gtataaagaa tatctacttg attaatgcaa
    25441 tatagctggc aaatatacaa ttgataagga gtaattttat aatggataat ttaattagga
    25501 tactgatatt agctgttatt ggtggattta taggttatgt tacgaatgta gttgctataa
    25561 gactgatatt tagaccaata gagcctatta aaatacctat tttaaatata gaaatagtag
    25621 gtcttatacc aaagagaaga gcagaaatag cagcaaatgt aggagaaata attcaagaag
    25681 aatttctttc tatggacgaa atacttgcaa atatcattac tgacgaagat aaggaagaag
    25741 ttgtaagata cataaaagca agggtaaaaa ttataataca tgagaaagtt tcatttatcc
    25801 cgagtggtat aaaaaacatg atacaagatt atctaggaga gataattgag tctgaagtta
    25861 agcaaagtat agatgaattg agtaaaaata taataaataa agcaaatgaa cgtatagata
    25921 tacaaaaaat ggtcgaagat aagattaatg aactagattt atatgaattg gaagagataa
    25981 taataagaat agctaagaaa gaattaaaac atatagagtt tttggggctt gtcttagggt
    26041 ttttaatagg tatagctcaa gggttaatta ctatgtttat ttaaatatat gagaagtagc
    26101 atttatttaa gtactaatta tatttaccta tttgagtgaa ttacttatta ataacattta
    26161 gatattaatt gtatttacct atttgagtaa attacttatt aatagcattt agatattaat
    26221 tgtatttacc tatttgagtg aattacttat taatagtatt tagatattaa ttgtatttac
    26281 ctatttgagt gaattactta ttaatagcat ttagatatta attgtattta tttatttgat
    26341 tgtgttactt attaatagta tttaagtatt tactgtacta cttattaacc atattaagta
    26401 ttgattgtat ttaagtatac aaaacctaaa aagagtacaa aaattagaag ttagttttaa
    26461 taaaaattag ttttagtatt tatctagtca tatctatcat gcatgtggat acgttttaaa
    26521 aattttaaat ttatgaaaat attataatat aactataaat caagagatta atttaatata
    26581 aaaatataag aatattggtt tacagctgat tgagatgaaa aataatgggt aattaatata
    26641 ttacattaat gttgattgat gtataactat ctgttataat gaaaatatat taaaaatcat
    26701 agtgttgaag aggaagagta gagtagaaac cattctcaga gaatttgggt ctatggctga
    26761 gagcccatgt aataggtcta tttgaaaact accttggagc tgagcttatt agcagtattt
    26821 gtatttatac tatacgtgaa agtgctcggg tgacgacctt atagtcatca agagggttgt
    26881 atatacttgt atacgccaac ttgggtggaa ccgcgaatta atatcgtccc aaacatattt
    26941 ggggcgattt tttattacaa aaaatctaaa aattatttca ataaggagga aaaaacaatg
    27001 attaaagttg ctttaaaaga tggctcaata aaagaatttg aaaatgctat ttctgttatg
    27061 gatgtagcta aatccattag tgaaggatta gctagaaatg tggtagcagc gtctgtgaac
    27121 ggagaggttg taggtctgga ccacattata gatactgatt gtgacttgaa tttatttaag
    27181 tttgaagata aagaaggtaa ggaagttttt agacatacat cagcgcatat attagctcaa
    27241 gctataaaaa gactttaccc agaagcaaag cttgctatag gaccaagtat agaaaatgga
    27301 ttctactatg atatagattt agaccataga ttagttccag aggacctaga gaaaatagaa
    27361 gctgaaatga aaaaaatagc taaagaagat ttaaaaatag aaagatttga acttcctaga
    27421 aatgaagctt tagagcttat gaaagaacaa ggagaagact ataaggtaga acttatttct
    27481 gatttacctg aaagtgaaat aatatctttc tacaagcaag gtgattttac tgatttatgt
    27541 agaggaccac atttaccatc tactaaaaag gtaaaagctg ttaaattaca aagtgtagca
    27601 ggtgcatatt ggcgtggtga cgaaaataat aagatgcttc aaagaatata tggaacttct
    27661 tttgagaaaa acaaagactt agaagagtac ttacatttac tagaagaagc taaaaagaga
    27721 gaccacagaa aattaggtaa agaattagga ttatttatga tacctgaaga aggtccagga
    27781 ttccctatgt ttttaccaaa aggtatggaa cttaaaaatg aattattaaa attctggaga
    27841 gaaatacaca gaaaagctgg atatattgag atagaatctc caatcatatt aaatagaaaa
    27901 ttatgggaga cttcaggaca ctggtatcat tacaaagaaa atatgtatac agttaaaata
    27961 gatgatgagg attatgctat aaaacctatg aactgtcctg gtggattaat ttactataac
    28021 tctcaactac attcatatag agatttccca atgagagttg cagagcttgg tagagttcat
    28081 agacatgagt tatctggtgc attacaagga ttaatgagag ttagagcatt tactcaagac
    28141 gattcacata tattcatgtt accagagcaa attaaggacg aaataaaagg agtagctaac
    28201 ttaatagatg gtatatacaa aacttttgga tttgaatata atttagaatt atctacaaga
    28261 ccagagaact ctatgggaag cgacgaagag tgggaagcag cagaaaatgg attaagagaa
    28321 gctttagaag agttaggtct accatatact attaatgagg gtgatggagc attctatggt
    28381 cctaagatag acttccatct aaaagattgt ttaggaagaa cttggcaatg tggtactatc
    28441 caattagata tgcagcttcc aagacaattt gataatactt atattggtca agatggtgaa
    28501 aagcatagac cagttatgat tcatagggtt gcttttggta gtatagaaag atttataggt
    28561 atattaatag agcattatgc tggtaaattc ccagtttggt tatctccaac tcaagttaag
    28621 atacttccta tatctgataa atttatggat tatgcaaatg aagttaagaa agagttattt
    28681 gataagggta taagagttga attagatgat agagctgaaa aaattggatt taagataaga
    28741 gaagctcagc ttgaaaaagt gccatacatg ttaatagtag gtgaaaaaga agtagctgat
    28801 aataatgttt ctgttagatc aagagataaa ggtgaaatag gtagtattaa attagatgaa
    28861 tttatagcta gtatttcaaa agaaattgaa tcaagagaaa gtattattca agactaatat
    28921 atattatatt agtagctaag atattgttat ataagataag tttaatcaat atgttgatat
    28981 gttgatgcat agtgattaaa tttatgaaaa gatgagtata attagaggta cagttaatca
    29041 tatttttatg attaatgtac ctctagtttt ttgtatggta gatttattaa taaatatatc
    29101 atagaagtgt tttgaacatg gaaatattta tgtgcattct ttaaataaaa gtttattttt
    29161 tgagtgataa ttaagtgctt gttggtaagg ttatggctgt aaaaatcata actttatttt
    29221 catatttgtt ccattttcat aaaaattatt atccctttaa gaaaatttct taatcctctt
    29281 gaaatatttc ctttttagtc taaagttaaa tttctttctt caaaatgaat atttaaatga
    29341 actaaaattt aagtacattt tctaatctta tgagtctatt tttcatagat aattgcccta
    29401 taatagaaga atctatgttg gtgctatgtg gtgacaataa cagcattgtc agtgattaat
    29461 taatgcttta taaatgatta attagtgttt tatgttattt ttggacttat cctctataca
    29521 tagattaatt tatttaactt tagatagaga gagaatgtca agtacataca cagacattag
    29581 agtttaagaa agtgtttatg gagcaagaac ttttaaaaga ggtagtgact tagtttaatt
    29641 tcaagaaatg ataaatacaa aagaggaaaa agagaggtgt tgaaatgata gaaaaagtag
    29701 aagaaaaatc taagattatt aaaacatcaa aagaatataa gcgaactata attgaagaaa
    29761 aagataaaga atggaccaaa aaattaaatg atatacttag tagaagtagt ttttctaatg
    29821 atgaattaga taaaatcaag gcattaattc caaaagagat tttgactaat gaaaatgttg
    29881 gagaagttgt gactgaagat ggatatgcta agcctgagta tgatgaggta tttaaatcat
    29941 tgttcacttt aaataatgaa tatgatttac ttgtaaattt tataaatgat attctaaaag
    30001 atgctccata tgctaacagg aatatcaaac cgttcactca aataaagaga ataattagag
    30061 ttgaaactga tccaacaatt aattacattg gagagaaaag acctaaactt gatattttgg
    30121 ctgaagatga agaaaataat catataaata ttgagatgca aagagctttt gagaacgatt
    30181 atttggaaag agctgaatat tatcttagtc gtgtacatgg tagaaagtta gaagaaggta
    30241 aagagtataa agaaattgga aaaactattg gaatacatat actaaatcat gtaaaatata
    30301 atcacataga agattatgta aattgcctga gattaacaat ggacgggcat ccagatattt
    30361 attctagtaa aactgcatta tattttatag agcttccaaa aattcataaa tctgactata
    30421 tagtaaatag aattatgcta tggggaaaac ttattagtaa tccttctaac ttagatgtaa
    30481 gagttatagc taagaatgat tctattatta aaaaagcact tgatagatta aaagaattag
    30541 gtagcaatga agaatattta ttaaatctga aaagaggtgc ttatatcatg aataaaagta
    30601 gaaattttaa agaagagatt gaaagagaga cagcaattag agttgcaaaa gaaaaagatt
    30661 ataaaataat tataatgttg aaaaaaaatg gttttaaatt agatgacata ttaaataaat
    30721 ttaatgattt tgacttaagt gaagatgaga taaaagaagt atatgaaaat aattgatgat
    30781 aaataaaaag tgaacttata taagtttgct gaatcggagt ctggaaatat ttatatgtat
    30841 tttttaatga atgaaaattt attttaaaaa caataattta gaagtacagt taattatatt
    30901 tttatgatac tgtacttcta gttttttcta ttgtagattt attatttact aataaatcta
    30961 caatagaaat gcttttgttg taagaatcat tttatctaat gattgttttt tataatagtt
    31021 aaatttccat ttgtagtgtt taaatataaa tctataacag catttctata aatgttataa
    31081 atcactataa tgattcaact caaagttact gcttaaagaa gcagagattt tatcaaggta
    31141 tatcatggaa aaaaatattt tattgtaata tttataagat tttattatag aaataatgta
    31201 aaaactttat aaataatata ttttatacct agttatagat aaatttatat agttattatt
    31261 aaaaaaagca ataaaaagtt taattcaatt tagcaaattt agtgatataa ttaaaaaaga
    31321 aaagaaatat ggacttattt ggaataattt tgacaatcta tttactacat gtgtagagtc
    31381 gtatataatt aaattataca taaaatataa aaaaataatt tagtaatacg ttaggagaaa
    31441 gtattatgat aaagtcaagc ttattttgga atattttcat tgcgatatcc tttatgatag
    31501 aatggtttct ttgtaagaaa atgctagatt atacaagtga gaaaaggatt tctaacaaaa
    31561 aaataaactt ttgtatggta ggtattattg tttttgctga cataatatat ttttttgatg
    31621 tacctccaaa tataagagta attatatcta tgataattac tattgcattt tataaaatct
    31681 catatgatgt ggggtttata aagtctgtat taatatcctt aatttattgg atgttactaa
    31741 tcgccacaga tgctttgagt atatctttaa cagtttggct taattctata aatgacatga
    31801 gtagtatggg gacgggagat atatacagaa ttgagtctat aatattggga aagatacttt
    31861 tgattgtgtc tttgctttta tatagagcct taaagataaa acttaatata gaaataaata
    31921 aaaggacatt attttatata gttattccta tagtcgctaa tatagcatct ttttttgtaa
    31981 tttttgaata catatttaat ccagggtctg gtagcttaat acataatttc caattcgtta
    32041 tgatttctgt tgtattgctt atatcaagta tcactttagt tttaattgct aaaaaattac
    32101 agcaatataa tactttagtt gtagaaaaca atatgattaa aaatactttg aagtatgaaa
    32161 atgagtatta tgaagatata gaaaagaaat atttaaaaac tagattacta agtcatgata
    32221 tgaaaaatca catattgtgt ataaatgcta tgattaaaaa aggtattgat gttacttctt
    32281 atttaaataa tttgcaggta gaaattcaga gtaatgactt gttatttaat actggtaatt
    32341 ttatattaga tgctatatta agtgaaaaga aagaggtttg tgataagaat gatatagaac
    32401 taaaaatagg aataaatttt agttgttgtg aagctgttga attagtggat atatgtcata
    32461 tattttcaaa tattttagac aatgcaattg aggcatgtaa taaaattgat agtggaaata
    32521 gagttatttg tgttaaaggt gatgttatgc aaaattttta tttaataaga gttgaaaata
    32581 ctaagataaa taagattatt actaaaaatg gagatatttt gacagacaaa aaaggttctc
    32641 ttcatggact tggaataaaa agtgtaaaaa actctgtaaa taagtatggt ggtaatgttg
    32701 taattgaata tgatgataaa aaatttgtag ttaaaattgc tattccactt agaaatgtga
    32761 agaaagcagt taatcatgtg atttaaatgt atataaattt gatgtgtaga aatttaatat
    32821 ataagaatta atgtataaaa gtgatgcata gaagtttaat atataagaat taatgtataa
    32881 aagtgatgca tagaagttta atatataaga actaatgtat aaaagtgatg cataaaagtt
    32941 taatatataa gaatttaata tgtataaaag tgatgcataa aagtttaata tataggaatt
    33001 taatatgtat aaaagtgatg catagaaatt taatatataa gaatttaata tgtataaatg
    33061 tggtgcatag aagtttaatg taaaaatttg gtgtataaga atttaatata tatatattta
    33121 ttttaatata taaattgaag atatataatt aaatcatata atgacatttg tatattagaa
    33181 ttgtttattg attattagaa acttttaagg ttagaaagtt tatgactatt atacattata
    33241 ttatttatta tatagtgagt ttaaaatatt aaacttttta ttctacaaaa tataaaaaga
    33301 agttgcatca aaaagagaaa tgctctttaa agatgtaact tctatggtat atgtatggag
    33361 gagctatatt gccatctctg gaatcaagct tctcatagct tctatagttg tttggataag
    33421 cttatcaagt tcccatccaa gcatttctgc accacttctt ataacgtctc gagaacagcc
    33481 agcagcaaat gatttatctt taaatttctt cttaacagat tttaattcta aatcagaaac
    33541 gctctttgaa ggacgcatta ttgcagcagc accaattaaa ccagtaagtt catccacagc
    33601 aaataatact ttctccataa attgctctgg tttaacatct gaaccagtta atccatatcc
    33661 atgacttatt gtagaatcga ttattgattt atctaaatca agctctgtca ttaattctct
    33721 ttgtttaaca caatgttcat caggatacat ttcaaaatct aaatcatgta aaagtccaac
    33781 tatttcccaa aattctactc tttcagggtc gtactccttt gcatagtatc tcataactcc
    33841 agatacaatt tgagcatgtc ttatatgaaa ttcttcctta ttgtattttc ttaatatttc
    33901 ccatgcttgt tctaaagttg gcatgtaact catttttatc actcctctag ctgaatattt
    33961 tgaattttta taaagtttat tttccattga ttatatcaat caatttctaa aacgtcaaca
    34021 tactgataca caattttgca tttaaattca gaaattagtg aaaattaaat ctttatatct
    34081 aaattctttg tatctaaaga ttttcgttcc tcaatgttat ctttgataca aatgctataa
    34141 gtaatgtaat aaatccaact cctagtataa tgatgtacac tctatctgat aataaatcaa
    34201 aaagtttacc atacataacc tgaccaaggg gcattgcaat tgctgttcct gcaaaaagaa
    34261 ttgacattac ttttcctaag agttcatttg gagtttttaa ttgtgtgact gttataacat
    34321 atattgagat aagagtcgtt gtaaaaaata ttagcataga tgatactata aagattaaaa
    34381 atggaactgg aaatcctgtg tttaatataa atggatatac tgaaaccgcc attggtatga
    34441 acataactgc acatataata aaggaaatat gcaaattagt gatttttaga tgttttgaga
    34501 aaataccaat actaacagca gctaccagtg aactaaaaga aattgctcct tctgctagac
    34561 catataagaa gttactagct cccatagtga tttttactat ataggggata ccaactatta
    34621 acatagagct taaaaataag tttattgcta tagctaaaaa aagtattttt agtataaatc
    34681 tattttcttt acatatatga acaataccat cctttatatc agtaaatata gtttttataa
    34741 tgttgctgtt ttgctcttgt ttgataaaag gaatctgtat aaatatctcc ataattgcag
    34801 atagaaagaa acttacacaa cttataacta taattgcatt aattccaatc acaccataca
    34861 agacacctcc taatacaggt cctgcaatat ttgtaagaga tgcaactcca gctacaatac
    34921 catttgcatt cattaaatgc tcattatcaa ctaaaagggg tgtacttgac tgaacagttg
    34981 gttgatacat tgtgcttatg actgataata tggtcataat aattcctaca agtaatattg
    35041 aaccattatt gttgaataag aatactgcta agattagtac tgtaagacta cttgaaaaat
    35101 caaaaataac cattaagttt cttctgttga atatatctgc aatagctcca ccaatagggg
    35161 agaatactat aattgggatt gaagatactg ctagtatagt agcaaatatt tctgctttac
    35221 cagttatatc aagtatgtat aaagataaag caaatttaag tattgatgca ccaaacaatg
    35281 agattatttg acctaatatc atcaagttga agtttttatt aaaagtgttc gaggatttta
    35341 ccatatatgt acctacctat ctaagattat taaaacattt acaataattg atataatagc
    35401 aattattata aggaattttt ttcttacttt aatatctaat attattagaa atatacaaac
    35461 tattatagat ggcttagagt agagtggaat gggtttgctc tagggtctat aagttttgtg
    35521 tgatatttag tttatcattt attttataaa ttatattgtt tattttttgc attaattcaa
    35581 tcatattaat cctatttagt ataaaaaaat acttagaaaa attattttaa attgttattc
    35641 aagtatgtgt tctacaagat tatccaccat aaagtcaaac acttcaatat agttatattc
    35701 aatattttct tttaccaaca gacttgatag tagagcatta caaacaccaa aaactttttc
    35761 tttttctatc ttcaacttga gattgtgtct ctcaattaag tcgtaaaaac tttgtatgtt
    35821 attggactca agttgtaaaa acatatcttg aggtagttta tttattaggc tgatgaaatc
    35881 aggattattt ggtgaatata aaaatggatt tttatcatat aatcgatata acatttttaa
    35941 ggattttgca aagccttctt ttggaggcat gttacttaaa atttcatcta ctgatgatat
    36001 aagtctgttt tggattccat ttaatacttc aaaaaataac ttttcttttg aatcaaaaaa
    36061 tgtatagaaa gaaccctttg caattcctga ttttgaacaa agttcatcaa tattagtttt
    36121 tttgtatcca tatttagacc agttgatttc acattctgaa attagtcttt ttcttatatt
    36181 gtctttttct atttctgtaa aacttttagc catttttatc aatcctttct gtctatatat
    36241 gatatatatg actttaaaaa tattttgagt catagatata taatattgtc tacttataaa
    36301 tttgtcaagt gatttatgtt tataatat
//
LOCUS       DAYAOY010000036        35348 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYAOY010000036 DAYAOY010000000
VERSION     DAYAOY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.036"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1143
                     /locus_tag="ACGI6B_001821"
     CDS             <1..1143
                     /locus_tag="ACGI6B_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HFG9751267.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="ACGI6B_001822"
     CDS             1397..3421
                     /locus_tag="ACGI6B_001822"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9751268.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="ACGI6B_001823"
     CDS             3641..4327
                     /locus_tag="ACGI6B_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFG9751269.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="ACGI6B_001824"
     CDS             4422..5477
                     /locus_tag="ACGI6B_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HFG9751270.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="ACGI6B_001825"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="ACGI6B_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFG9751271.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="ACGI6B_001826"
     CDS             7194..8900
                     /locus_tag="ACGI6B_001826"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFG9751272.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="ACGI6B_001827"
     CDS             8922..9995
                     /locus_tag="ACGI6B_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HFG9751273.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="ACGI6B_001828"
     CDS             10007..10729
                     /locus_tag="ACGI6B_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9751274.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="ACGI6B_001829"
     CDS             10745..11902
                     /locus_tag="ACGI6B_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HFG9751275.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="ACGI6B_001830"
     CDS             11905..12744
                     /locus_tag="ACGI6B_001830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9751276.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="ACGI6B_001831"
     CDS             12759..13934
                     /locus_tag="ACGI6B_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFG9751277.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="ACGI6B_001832"
     CDS             13963..14712
                     /locus_tag="ACGI6B_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9751278.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="ACGI6B_001833"
     CDS             14734..15642
                     /locus_tag="ACGI6B_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9751279.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="ACGI6B_001834"
     CDS             15690..16442
                     /locus_tag="ACGI6B_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9751280.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16396..17763
                     /locus_tag="ACGI6B_001835"
     CDS             16396..17763
                     /locus_tag="ACGI6B_001835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HFG9751281.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17769..18860
                     /locus_tag="ACGI6B_001836"
     CDS             17769..18860
                     /locus_tag="ACGI6B_001836"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9751282.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="ACGI6B_001837"
     CDS             18944..20857
                     /locus_tag="ACGI6B_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFG9751283.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="ACGI6B_001838"
     CDS             21056..21763
                     /locus_tag="ACGI6B_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9751284.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="ACGI6B_001839"
     CDS             22065..24176
                     /locus_tag="ACGI6B_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HFG9751285.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="ACGI6B_001840"
     CDS             complement(24374..25339)
                     /locus_tag="ACGI6B_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFG9751286.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="ACGI6B_001841"
     CDS             complement(25624..26580)
                     /locus_tag="ACGI6B_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFG9751287.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="ACGI6B_001842"
     CDS             26948..27736
                     /locus_tag="ACGI6B_001842"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9751288.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="ACGI6B_001843"
     CDS             complement(27859..28329)
                     /locus_tag="ACGI6B_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HFG9751289.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="ACGI6B_001844"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="ACGI6B_001844"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HFG9751290.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="ACGI6B_001845"
     CDS             29155..30060
                     /locus_tag="ACGI6B_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9751291.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30375..31922
                     /locus_tag="ACGI6B_001846"
     CDS             30375..31922
                     /locus_tag="ACGI6B_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9751292.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31925..32323
                     /locus_tag="ACGI6B_001847"
     CDS             31925..32323
                     /locus_tag="ACGI6B_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFG9751293.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32363..34096
                     /locus_tag="ACGI6B_001848"
     CDS             32363..34096
                     /locus_tag="ACGI6B_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9751294.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34328..>35348
                     /locus_tag="ACGI6B_001849"
     CDS             34328..>35348
                     /locus_tag="ACGI6B_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9751295.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKL"
BASE COUNT        14110 a         3723 c         6228 g        11287 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgttaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatttt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta tagaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagtaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggttcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaggaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa taggatatgg tggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaag ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaacta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagcgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa tccagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aataatccac aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaattg cttagaaaat ataaaaataa
    19981 attagattta aaaacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatt attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtacttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat ataaatgggg agcaactgga ccaaattctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca cgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggatgattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttga aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatga
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatatata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagcatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggtcagta cttattatgc aaagtttaaa attgtagaag atttgacgaa
    27361 agtagaagga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 tataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgcc ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatagat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaaatcctt tgcatcaaag atttttgatt cctttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggactaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatggttg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agacttgata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actaatcaac aacaaaatgt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttgcaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaattttaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaagaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttactcac aagacatatt gaggtagata
    30481 ttaaagtttt aattttaatt ttattaacct tgtttagtat aaatgtattt atttcatatc
    30541 ttaaaattaa gttgtatgag gataaaatta atttaggatt gataaataat tcagataagt
    30601 ttataaatat tactaaagat gagaacttcc taaaacatgt tcaaaattat attatcagta
    30661 atgaaaaaga aaactgtgta atggcttgtt ttgatttatg tagactcaaa cttataaatg
    30721 atatatatgg ttatgagcta ggggatgaag tgttaaataa tattttaagt aacttaaaaa
    30781 attattttgg aaaagaagct atttatggaa agttaaaaag tgatgttttt gtattaataa
    30841 taaagcttga aaatagggaa cagaagattc cttatttagt aaatcttatt aagaataaaa
    30901 ttgtgatact aagtcagatt gacagtttta aaatggatat aaatattgag gcttctattg
    30961 gaatttataa atttaacaat aatgaaattg atataaaaaa agctatagat aatgctgata
    31021 tggcaagact taaatcaaaa ggactaaagc acatagagta tgtagaattt gataatgcta
    31081 tggaagaaga aatacaatca attatgaaaa tagagagaga tttattcttg gctattaaaa
    31141 ataaagagtt tgtaattcac tatcaaccta aagttgattc aagtacaggt aacattatag
    31201 gttcagaggc attgataaga tggttgcacc catcattagg catggttgga ccaaataagt
    31261 ttatacctat agctgaaaaa aatggtctga taaacaatat tggtagatgg ctaattcaag
    31321 aagtttttat taccattaac aagtggattt cagaaggtat aaatgtctta ccagtctcaa
    31381 taaatctttc tagagtagaa ctatataaaa atgatttagt tgattttttt aaactgatgt
    31441 ttaatacata taatattcca aaagaactta tagaattaga aattacagaa acgacagcac
    31501 taagagatgt aaaatttata agtgaaaggc tttatgagat taaatcttta ggtatgaata
    31561 tatcattaga tgattttgga gtaggtaatt caaattttat caaccttaag gggataccac
    31621 tagatattat aaaaattgat aggtcactta tattggatat agttacaaat acaaaaacaa
    31681 agtttatggt gaaagcaatt gttaaccttt cacatgattt aaacgtaact gttatatgtg
    31741 aaggggtaga ggatatgcat caggtcaaag tcttaagtga actgggatgt aatttcatac
    31801 aaggttatgt attttttaaa cctttggatg agatgaatta taaaaaactt cttactgacg
    31861 gaagttatat aaatctgaaa gatactcttt tagataaatg catgacagtt gaggaggagt
    31921 aaggatgatt tcagaagatg tagattattc aataagaatc ataagagcta tatttaataa
    31981 tggagaagat aaaccaatag atgctaaaat gatattaaaa agtgaagata taccaatact
    32041 aacactatac agactgctta gaaagctttg tgcaaacaat attcttactt ttaacaaaga
    32101 agatggcaag acatacatgt taaatgtgtc attggataca ataagtctaa gaactattgt
    32161 agaagtaatt gatggaaaaa tatatataaa taaatgtacg agatatgtat ctaattgcag
    32221 taattgctta aaaggttgta aagtaaatcg tgaattgcta gaaatagaaa aagaaattta
    32281 taaactttta tcgagaaaat ctataagaca aatactagaa taaaaaattt tcagctatga
    32341 taataattgg aggttttcgg ttatggatga tatatctcaa gataattttc tactgtctaa
    32401 agagtatgag aatagcttag atgtagatac taaaaaagca agtggaatat attatactcc
    32461 taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata taattaaaaa
    32521 tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt tagaagtata
    32581 tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga agtatgatga
    32641 aaattattgg actgttgata acatacaccg tcatatatta aactattgta tatatggtgc
    32701 tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata aaaaggtagt
    32761 aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata gtcttaaaaa
    32821 gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg gtcataaaaa
    32881 acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt ataaggataa
    32941 agctgactta tatttttgtt tttataaaaa gattatagat attttaaagc aaggagggat
    33001 tggcagtgtt ataactccaa gatatttttt agaaagtcta tcaggtaaag atttaagaga
    33061 atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg caaatatatt
    33121 taaaaatata ggggtatcta gttgtattct aacttttgat aagaaaaaaa caaaagagac
    33181 atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat ttgaaacatt
    33241 ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga gattgctatc
    33301 tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga tacaagaaaa
    33361 atgtaaatat agcttggaag atatagcaat aagttttcaa ggaataatta cagggtgtga
    33421 taaagcgttt attttaagta aagatgatgt aaagttgaac cttgtagatg ataaattttt
    33481 aaaatgttgg ataaaaagta agaatataaa taaatatata gtagataaaa gtgaatatag
    33541 gctaatatac tcaaatgata tagataatga gaatacaaat aaaaggatac tagatgaaat
    33601 tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa
    33661 atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa agataatgta
    33721 tccttataaa tcaaatgaaa atagatttgc tatagattat gataataatt tttcaagtgc
    33781 agatgtatat tcttttttta taaaagaaga gtatttagat aaattttcat atgaatatct
    33841 agttggaatt ttaaattcca gtgtttatga taaatacttt aaaataactg ctaaaaaaat
    33901 gagtaaaaat atatatgatt actatcctaa taaggttatg aagataagga tttttagaga
    33961 taataattat gaagagatag agaatttatc aaagcaaata atatcaattt tattaaataa
    34021 gtctatagat aaaggaaaag tagaaaaatt gcagataaaa atggataact tgataatgga
    34081 ttctttaggt atataagcag ttgtaagtat taaaaaatta taatctttta attgtttatt
    34141 taaatttaga aataaaaagt tagccatata aaaaatatta aaaatatata cttttcaata
    34201 tttttttgat aaaataagaa tgaatttgtg caattttatt tttctccaat aactaaaaat
    34261 agttttttga attaatattt gcttaagaat aaaaatcaga aacgtttttg atatagtagg
    34321 tgataatatg aaaggattag ttttagaagg tggaggaaca aaaggagcct accaaatagg
    34381 tgcatataaa gctttaagag agcttggtat agagtttcaa ggagtaactg gcacatcaat
    34441 tggtgcttta aatggagcat atatagttca aaatgatatt gaagtaatgg aagatatttg
    34501 gttaaattat gactatactc attttatgaa tattgataga gaaacctatg agaggtttaa
    34561 aaatgtagat ttcactacta aaaatctcaa tgcagtgttt gagcttataa ataaagctag
    34621 aaaaaatgaa ggtatagata taagcccatt aagacaacta atggagacaa cattaaatga
    34681 agacataata aggaaatcaa aaaaagactt tggacttgtt acggtctggt gggataaaaa
    34741 aattaatcca tatccattgt atatcgaaga tatacctaaa ggaagattga ttgattattt
    34801 aattgctagt tccagtttgc ctatttttca aacgaacatg atggatgata agttgtatct
    34861 tgatggaatg ttttgtgata acatgccaat aggactcttg gaagacaaag gttatgaaga
    34921 tgttgttgta attaggttga ttgatgattt tattgggaaa atgaatctaa atcgacatca
    34981 aaatataaat ataaaaacta taataccatc agaatatcta ggaggttctc ttaataaaga
    35041 tagagataat gtagaaaaaa acatccgact aggttactta gatactatga aagcatatga
    35101 aagatgcact ggaattaagt attattttga tgtagagtat aaatataatg aagaatattg
    35161 ttttgataaa tttagaagta tgagcaatga aacaatacaa tatatctgta atttattgaa
    35221 tataaaaaga gatgcttcac ttagaaccgt tatggaaagt gtaataccaa agcttggtga
    35281 ggtgcttgga ttgccaaagg atttttcata taaagatttg ttttactgta tatatgaaaa
    35341 gaaacttg
//
LOCUS       DAYAOY010000037        34893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYAOY010000037 DAYAOY010000000
VERSION     DAYAOY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.037"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..894)
                     /locus_tag="ACGI6B_001850"
     CDS             complement(<1..894)
                     /locus_tag="ACGI6B_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751296.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELS"
     gene            1818..2669
                     /locus_tag="ACGI6B_001851"
     CDS             1818..2669
                     /locus_tag="ACGI6B_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFG9751297.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            2673..3143
                     /locus_tag="ACGI6B_001852"
     CDS             2673..3143
                     /locus_tag="ACGI6B_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751298.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            3156..4466
                     /locus_tag="ACGI6B_001853"
     CDS             3156..4466
                     /locus_tag="ACGI6B_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9751299.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            4678..5424
                     /locus_tag="ACGI6B_001854"
     CDS             4678..5424
                     /locus_tag="ACGI6B_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9751300.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            5786..7327
                     /locus_tag="ACGI6B_001855"
     CDS             5786..7327
                     /locus_tag="ACGI6B_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9751301.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            7362..8468
                     /locus_tag="ACGI6B_001856"
     CDS             7362..8468
                     /locus_tag="ACGI6B_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9751302.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(8847..10133)
                     /locus_tag="ACGI6B_001857"
     CDS             complement(8847..10133)
                     /locus_tag="ACGI6B_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9751303.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(10256..13354)
                     /locus_tag="ACGI6B_001858"
     CDS             complement(10256..13354)
                     /locus_tag="ACGI6B_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9751304.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            14080..14238
                     /locus_tag="ACGI6B_001859"
     CDS             14080..14238
                     /locus_tag="ACGI6B_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751305.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(14525..15229)
                     /locus_tag="ACGI6B_001860"
     CDS             complement(14525..15229)
                     /locus_tag="ACGI6B_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751306.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(15220..16827)
                     /locus_tag="ACGI6B_001861"
     CDS             complement(15220..16827)
                     /locus_tag="ACGI6B_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HFG9751307.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(17081..18259)
                     /locus_tag="ACGI6B_001862"
     CDS             complement(17081..18259)
                     /locus_tag="ACGI6B_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751308.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(18485..18979)
                     /locus_tag="ACGI6B_001863"
     CDS             complement(18485..18979)
                     /locus_tag="ACGI6B_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9751309.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(18980..19741)
                     /locus_tag="ACGI6B_001864"
     CDS             complement(18980..19741)
                     /locus_tag="ACGI6B_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9751310.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            20443..23277
                     /locus_tag="ACGI6B_001865"
     CDS             20443..23277
                     /locus_tag="ACGI6B_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751311.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            23320..24564
                     /locus_tag="ACGI6B_001866"
     CDS             23320..24564
                     /locus_tag="ACGI6B_001866"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9751312.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(24979..26721)
                     /locus_tag="ACGI6B_001867"
     CDS             complement(24979..26721)
                     /locus_tag="ACGI6B_001867"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751313.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(26705..27379)
                     /locus_tag="ACGI6B_001868"
     CDS             complement(26705..27379)
                     /locus_tag="ACGI6B_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751314.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(27416..28405)
                     /locus_tag="ACGI6B_001869"
     CDS             complement(27416..28405)
                     /locus_tag="ACGI6B_001869"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFG9751315.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(28453..29697)
                     /locus_tag="ACGI6B_001870"
     CDS             complement(28453..29697)
                     /locus_tag="ACGI6B_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9751316.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(29739..32285)
                     /locus_tag="ACGI6B_001871"
     CDS             complement(29739..32285)
                     /locus_tag="ACGI6B_001871"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9751317.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(32493..32654)
                     /locus_tag="ACGI6B_001872"
     CDS             complement(32493..32654)
                     /locus_tag="ACGI6B_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751318.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(32710..33726)
                     /locus_tag="ACGI6B_001873"
     CDS             complement(32710..33726)
                     /locus_tag="ACGI6B_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9751319.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(33723..>34893)
                     /locus_tag="ACGI6B_001874"
     CDS             complement(33723..>34893)
                     /locus_tag="ACGI6B_001874"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9751320.1"
                     /translation="FLLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNN
                     LNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPH
                     IIITGGEPLIREDFFEIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISI
                     DGVDENTCSETRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKK
                     LGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCAC
                     NAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIY
                     PYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
BASE COUNT        11995 a         5511 c         3997 g        13390 t
ORIGIN      
        1 agacaactcc cttagtctag ttattacatt atccctaaca tctttaggtt caagtacttc
       61 aacattgtca ccaaatgata aaatatatcc ataaatccat tcaccaatag gaaattctgt
      121 aattacatca tatgtatcat cttcattttt agttattaaa tccttattaa aatcatcaaa
      181 tactctaaaa agcatctttt ctttaaacct caatttcaac gttatagtat tttccttaat
      241 tactttagaa tcattcaaac atacttcttc tattatcttt cttgaaaata cttcatcctt
      301 tatagataaa ttccttatcc tactaattct aaatatccta aaatcatctt ttagtttaca
      361 aaatccatac aagtaccaag tcttactctt atacataagc ttcagtggtt cgacagttct
      421 gtttgtctgg cttgacaaac tatttacata attaaaattt atagtattac gccttaaaat
      481 agcttctttt atttttgtaa atttatcatc ttcattaaaa ttacttcccc attcagtaaa
      541 gtctatttca atccagtttg aaaaattctg ctctccaaat attgagccaa ttttatttac
      601 tactgaattt atttctggat acttagtcgc ctgtaatgtt ttcagtgcaa ctattaaact
      661 ctctttatct ttttttgaca atatagcctt gtctatagaa tattcttcta ttaaagatat
      721 ccctcctcct ttgcctttcg tcatatatac tggtactccc gacatagaca gagtttctat
      781 atctctatat atggttcttg tagaaacttc aaatctatct gccaactctt tagctgttac
      841 taatttttta tttaatagaa ttactattat ttctgttaat ctatttattt tcattcatta
      901 ccacctacta aaattattaa atttatttaa tctaaattaa tcatactaat ttttaaataa
      961 ataaaatctt agaatcttat ctaaattatt tttacatgat tttatttact ttctattatc
     1021 tttctcactt tggatattat catcttaatt tatttattac tatatcaatt tacttattat
     1081 catctcattt gttatcgtct tacttaattt acttatttct atatcaattc acttattatt
     1141 acctcacttg ttatcatctt acttaattta cttatttcta tatcaattta cttattatca
     1201 tctcatttgt tatcgtctta cttaatttac ttattgctat gtcaattcat ttattattat
     1261 ctcacttgtt atcgtcttac ttaatttact tatttctata tcaattcact tattatcatc
     1321 ttaattcatt tgcttattat catttacttt attcaatttc tatatcaaaa ttagtatttg
     1381 tacttaatac ttaaaattaa tttaaagtct ttacatatta tttattttct attgtaatat
     1441 tcatttccat actttactaa gctacttcca ctcatttatt ttaaaatttc aatttactat
     1501 ttctcaactc tatattatta tttttcaatt ttatattact aattttaaag ctttttagct
     1561 attattccta caatatttaa acaatttttt cataataaag ctatattaca atatattcaa
     1621 aatatgtaat attttagata tttttcgttg acaatgttaa atttttgttt tatccttaat
     1681 taaggacatg ttattgacat ttctgaaact tatttgaaat agttttttca ttttatttag
     1741 gaaaacgtat tatacttaag aaaacgtatt ttctcatttc agataagtaa aatatttatt
     1801 cttttgggga ggaaatcatg gaaattaata aaaaacattt tattcaagta tttttaactt
     1861 gcttaatctt ggtagtaata tgtgaattta tagggcaaag aactatagct attggaaaag
     1921 cctctatagt actgttccct atgctttatg cagttataat tggacttata ataacaccag
     1981 atttattagg taaaaaaatt aaagcactta aaaaagtggt tggagaaaaa gaaataaata
     2041 ttgctggtga agttgttgga attgcactag taatgcttgg tataaaatat ggtactacag
     2101 ttggaccaaa cattgacaaa ataattcaag ctggaccagc gtttatcgct caagaatttg
     2161 gtcatatact tgcaccaatc gttgcattac ctcttgcatt aatgtttgga atgaaaagag
     2221 aagctgttgg agctactgca agtataagtc gtgaaccttc tttaggagtt atatcagaaa
     2281 aatatgggat aaactcacca gaaggtagtg gtgtacttgg tacatattta atgggaacag
     2341 ttcttggtac tatatacttt agtgtactag gttcaatatc tatttatagt ggtcttcacc
     2401 catatgcttt gggaatggcc tgtggtgttg gtagtggaag tatgatgaca gcagctgctg
     2461 gttcattatc taccatggta ccacctgaaa tggcagatac aatacttgcc tatgctgcaa
     2521 caagcaatat gatgtctagt ataacaggaa ttatattctt agtttttgta tccttaccat
     2581 ttactaattt tatgtataag attttagaac ctaaattttc gagaaataaa aaaactaaag
     2641 aaattaaatc tacagaagga gagtgctaat aaatgtttgc taatttaaat ttaaagaaaa
     2701 gttcatatca attattggtt atgtctgtta ttggagttat aattcttata ggacaaagaa
     2761 ttagcgttgg tacatctata gttacagctc ttcctggtat ggtgatgcta atattagctg
     2821 ctatggcagc tatgattata aaagatttat ttcctaaatc aatctttcca gcttttggat
     2881 ttgcaactat aatcggcctt ttacttagta tgccatatag tccaacatct gaagtattct
     2941 taactaacac taataatatt aactttatgg ctataactac tccactacta gcttttgcag
     3001 gaatttctgt tggaaacaaa atagaagctt taaaagaaat gtcatggaaa atagttataa
     3061 tttcacttat tgtatttaca actatattct ttgcatgtgc gagtatagct catatagttt
     3121 taagtattca aggtaaaata taggaggaat ttattatgaa taaagaacaa ttaaaatctc
     3181 tagtaataaa aactatagat gaaaatagag aaaaaatatt aaaaataggt caaggaatat
     3241 acaaaaatcc agaatatgga tataaagaat ttaaaactac agaagctgtt tcaaactttt
     3301 ttgagcaaga attaaattta tctgtagaaa aaaatatagc agttacagga tgcaaagcag
     3361 atgcaaattc tagcaagaaa ggtcctcata tatctatact tggagaattg gatggtatat
     3421 catgtaaaga acataaagat gctaatgaaa taggtgcttc tcatacttgt ggacataata
     3481 ttcaaatagc tggaatgtta ggtgctgctg ttggtttagt aaaaagtggc gttttagaac
     3541 atttagatgg taaagtatct tttatggcta ctcctgctga agagtttatt gagttggaat
     3601 acagagagca attaaaaaaa gaaggaaaaa taacttattt tggtggaaaa caagaattaa
     3661 taaaaagagg ttattttgat gatatagaca tatctatgat gtttcatgct tctgatttag
     3721 gagaagataa ggctctagtt ggtcctgaga gtaatggatt tgttggaaaa aaggttaaat
     3781 ttataggtaa agaatctcat gctggttctg ctccacatga tggaataaat gctttaaatg
     3841 cagcaatgct tgctataaac aacgtgaatg cacttagaga aacttttaaa gagtctgaaa
     3901 gagttagatt ccaccctatc atcacaaaag gtggagatat agtaaatgtt gttcctgctg
     3961 atgttcatat ggaatcatat gttagagcta gaactataaa tggtatgatt gatgctaatg
     4021 aaagaataaa taaggctttg atggctggtg gaatggcagt tggagctgat gttgagataa
     4081 ctgaaattcc tggatatctt ccaatattac gatacaaaag tcttgataat ttatttaaaa
     4141 acaatcttga agatttaggt ctaaaaggaa aagttattga tggtggagat tttacaggct
     4201 cttttgattt tggagatgtg tctcatataa tgcctactct tcaccctatg attggtggtg
     4261 taaaaggagc tcttcataca agagattttg aaatagttga tgaagattta gcttacataa
     4321 ttcctgctaa agcaatggca cttacagttg tagaccttct ttttgataat gctaaggatg
     4381 ctaaagatat attagatacc tttactccag ttatgactaa agaagaatat ctaacattct
     4441 tagaaaaaca cgataaaact ttataagtct atttttataa tagggtggta tctactgtaa
     4501 aagttatacc actctttgtt tttgtaacag tatacttctt ttcttttagt ttttagatta
     4561 aactaaaata tattatcctc cttaaaactt ttagagctac gttgaaatat cagtagaact
     4621 atgctatatt ctttctaatt aatataaatt tctattctaa ttcaaggtgg tgaaaaaatg
     4681 tttaatgcag gaaatcgaat tataaataca tggttgtacc ctattcaaga tggctttgtc
     4741 ttgattgata ctggatatga aaatggtttt aaacatttca aaaaacgaat ttctaaattc
     4801 aacataaaaa tcaaagatat tcgatacata tttctaactc atgctcatga tgaccatgtt
     4861 ggttttctaa atcagattct agacgaatct cctaatacaa agattgttat gcatgataaa
     4921 gctttagaaa ttctatataa aggtcaaaac tcttttattg gtggttgtac cagccgtact
     4981 gcctttctct tttgccaaat tatgaaacta tttggaaaag gtgagcatag tttttctcca
     5041 cttaaacaag aatttgaaaa aaggtgtatt cttgtgtctg acaagaatcg taaagaaatt
     5101 gaaaaagaac taaatggaaa aattatagat acaccaggac atactgatga ttcaatttca
     5161 ctactaattg atgaaggtgt tttattttgt ggagattctg caatgaatgg atttccaagt
     5221 actcacaaaa ttaccatatt tgcagaaagc aaactagaat ttattcaatc ttggaaaaca
     5281 atcatcaata caaaacctaa aatgatttat cctggacatg gaaaaccatt tgaatacagt
     5341 aaattagaac aaaatctaat ctatgtaaat aaaataaatt tatatcctct acataattca
     5401 aaagatattc acagattttt gtagaaaata caaagtaaat ttatttgatt aattaaaaaa
     5461 tctaacactt tactacgcta cttattttaa taacttaatc ttccactagc ttttcataaa
     5521 attttgtttt aaaattaatt ttaaagtgca tatgttattt aaatacatta gataaaattc
     5581 gtcattaatc tacttaaagc atatttaact aacaccactc aaccagaaaa ttcagaaaac
     5641 tgcattgaca caggaaaaag tttattttat acttttttta ggacatattg tggacacgtc
     5701 ttaaatcgac aaacagaatt taaattgtaa gattaaccac aatacttatt ttttaattat
     5761 atttttaaac attagggagg atattatgaa tgataacgta gttaagaaga aaaacttttt
     5821 tgacaggaca ttaaacagaa tagaaactgt tggtaataaa ctaccagacc cagttacaat
     5881 atttttaggg ttatgtgtac ttttacttat actatctagt ctagttggtt ctatgggtat
     5941 atcagtagtt catcctggta ctggagaaac tataaccgct gtaaatttat tgactgtaga
     6001 acagcttcaa attcttttag gaaacatagt cagcaatttc caagggtttg ctcctttggg
     6061 tctagttcta gtaactatga taggtgctgg tgtatgtgac aaaacaggac ttatgacagc
     6121 aacaatcaaa gcatcagtaa gcaagattcc tgaaacaaga gtaactcttg ttgtaatgac
     6181 tataggtatg cttgccaata tagcttcaga tgcaggtaca atattatttc caccattagc
     6241 tgcacttgta tatcttggtg ttggtagaca tccacttata ggtttatttt ctggatatgc
     6301 agcagtctgt ctaggatttg ccgcaaacat tatgataagt gtcaatgata tacttgctgc
     6361 aagttttaca gttccagcag ctcaaatgat tgattctgga tttcaagcca atgcaacaat
     6421 gaacttattt tttatgatag catcaacttt tgttctaata cttttggcaa catgggtaac
     6481 agaaaaaatt gtagctccta gatttggtaa atatgaagga aatgcagaac tagatgttga
     6541 tggaagtctt agtgatgtag aaaagaaagg gcttaaaaaa gctagtataa gtatattaat
     6601 atatgtggct attatagtag ctcttagtgt aatcggtaaa aaaccattct tagctgaccc
     6661 agaaacagga gatttactat caaacaatgc tcctcttatg aaaggtatgg tacctataat
     6721 agcactagca tttttcatac ctggtgttgt atacggaaaa actataggta aaataaagaa
     6781 ggataaggat gtagtctcta taatggctag tgcaatgagt gatatgggtg gatatataat
     6841 actagcattt gctgcttcac aatttttgca attattcaca aatagtaatt taggtctgat
     6901 actagctgta aagggcggag aatttttaaa atcagcagga ataaatggac ctttattgtt
     6961 gataggattt attatattat catgttttat aaatttattt ataggtagtg catctgcaaa
     7021 gtgggctatt cttgcaccta tatttgtacc aatgtttatg atggttggat acaatccagc
     7081 acttactcag atggcttatc gtataggtga ttgtataaca aatccactta gcccattatt
     7141 tccgtacttc cctattatac tagcattcac aagaaaatac gataaagatg ctggtatggg
     7201 tactgtaatt gctaatatga taccatactc tatatcattc ttaatagtgt ggactatact
     7261 attaatattg tttatggtat ttaatattcc actaggacct ggaatacttc cttcatatag
     7321 catgtaattt atttaaatct ttctaaaagg agaaataatt tatgttagat aaaaatagac
     7381 ttataaataa ttttatggat atggttagaa ttgatagccc atctaatcaa gaattagaaa
     7441 tgtctaagtg gttggtaaac tacttaaaag aaagaaatat agatgccatt atagatgatg
     7501 caggtgaaaa atatggtggt aatacaggta atgtcattgc ctatataaaa ggtgaagaag
     7561 gaagtagacc actttgttta tgtgcacaca tggaccaagt tcaaccttgt ttaggtgtaa
     7621 aaccaatact agatggtaat gtagttagaa gtgatggaac tactacactt ggtgcagatg
     7681 ataaagctgg aatagctgct atacttgaag ctttagaaca tgttattaca gaaaaaatac
     7741 cacatagaga tatttatctt tgttttacaa tctgtgagga agctggtatg catggtgtca
     7801 aaaactttaa tcctgacaac ttgccttgta aagatatggt gattttagat tctggtggag
     7861 ctattggttc gatagcatac aaagctcctg ctcaacaatc tataaaaatc tcttttcaag
     7921 gaaagaaagc acatgctggt atagaacctg aaaaagggtt aaatgctata ttagtagcct
     7981 ctcatgcaat aagcaacatg cacataggca gaatagattc attgactact tcaaatatag
     8041 gtgaaataga aggtggtgga gctacaaaca tagtaactga taaggttaca ttgactgctg
     8101 aaattaggtc tcatatacct gaaactttag agtatgagct taatcatatg gaaaaatgtt
     8161 gtaaggacgc tgcatctaaa tttaatacta cttatacttt tgaacataat atgtcttacc
     8221 ctagttttga acttagtaga gatagtcatg ttttcaagtt aagtgaagaa gcaataagac
     8281 aagttggtgt tgttccaaat ccaatggtaa ttggtggtgg aagtgacgca aatatattag
     8341 ctaatcttgg atataattgt gctatattaa gtctaggtat gtatgatgta cacacagtta
     8401 atgaatatgt aaatatagac gaattatatg atactgccaa aatagtttat catatgatta
     8461 aattataaat atcataatag aactctttaa attataaaaa taaaaaccta tgtatcctca
     8521 actttataag ttatatatta gagttgtttt acataggttt tagtattttt atataataat
     8581 ttgttttaac atttttcata ataagctttg atattttata tagtaatttg ttctaacatt
     8641 tttcataata agcttcaata ttttgcataa taatttattc tagcattttt aataataggc
     8701 tctagtattt tatattaata atttattcta gtattttaca taattatttg ttctaatttt
     8761 tttcataata agctttaata ctttatatag taatttgttg tagaatttta tacaatataa
     8821 tgatttatac agttttataa aatatcttaa aacagtattt cagtaatatt ttttggtctt
     8881 cctccaccaa tactagtctc ttttgcacat attcgaccta aacctgcatt tactatttta
     8941 tttaatatac gtcttgcact tctttcactt acatttagat actctgctaa ctcttttgaa
     9001 tcataaatct tactttttct attctcattt atagcaataa tttttgcaac tgactcacaa
     9061 ctcattccag tctttttaga tatttcgatt accttttcat cagatagcat caaagaataa
     9121 tttaattcat tttcggaagc taaaggtcct cttatgtttt catcttcatc taccaaatat
     9181 ataccaattt cttttgaatc tatggagtgt cttaatgctt tatgtgcatt attttcagcc
     9241 tgatacgcag ttactccagt accaattcct atactaagag aaaagcccat gtcttttatc
     9301 tctctcttta gattgaatag cttatcataa ttatactcat tatcagctac ccctttatga
     9361 acaaatacaa cataatcatt tcttccaagt aaaaataagg aaccttgaat gcttcttaca
     9421 tagtttacta caagcttatc catatctgat ttttttatca tgtcagaata atagttatct
     9481 atcttctctt tataatctat taaactcaat atttcaacag caatttggga aaatcttgcc
     9541 ttacttagag catattcact ctttaccttg tcataacttt ctttgattaa ttgtatagta
     9601 gcttgtaaac gaaatacagg atagcctctt tcttttaatt cattatatac tgcaccaaaa
     9661 ccagtcaaca ttatatccgt cttattttct tcaaataatt ttatatgcca ttctacaagt
     9721 tcagactctc ctacttccaa agaaaatgga ttacaaaata cactcgttgc tttaatatca
     9781 aattctttaa gtgcatcttc cacaacatat ctatcaacta catctatact gaatttatct
     9841 aattttatat ttgcactttt tattgcccaa aatgccttca ttatactcgt cccacttctc
     9901 ggtacaaatg aatgtggctt acttatctca tacttagatt ttataaattc ataaactgca
     9961 catcctgtaa atataatagc atcacattcg tcttcacatt tagaaataac ttctattgta
    10021 tctactactt tttctcttac atatatttta gtctttaaat cactgtctat ctgttttaaa
    10081 tcatccttta tcttaaaccc agagtctgaa ggacctataa tacccactat cataaacttc
    10141 gctcctaatc cataaatatt atacttttta aaaattatat cctcactatt ttctacttct
    10201 atttaattat tttgaactac tccatcctac ctacatttag aatggaataa ttagcttatt
    10261 tttgataaag taacttctca aaatcagcac ttggaatagg cttacaataa taataacctt
    10321 gaattaccac acaatctaaa gtcactaaaa attcagcttg ttcttttgtc tctacacctt
    10381 ctgccactac atccatatct aaatcttttg ccatggctat agtgtgcttt aaaactattt
    10441 tttctctgtc tgtgattcct tcctcattaa agaaaccaca atctatcttt aatgtatcaa
    10501 tattaatatt ttttaacata tttaatgaag aatatgctga accaaagtca tccatattta
    10561 aaacaatacc atccattcta aggtcggcta ttatcttagt aaataatttt atatttttaa
    10621 taaataaact ttctgtaagc tctaattcta aaaaagattt tggtatacta tatttattta
    10681 ttaaacttaa aacaatttct ttaaaatgtg gattatgcaa atgtaatctt gatacattta
    10741 cagagatagg aactggctct tcacctaaat ctatccattt tctaatcaaa gaacatactt
    10801 gttcccatac atacatatct aatctgataa taaacccatt cttctcaaat agtgaaataa
    10861 atttaattgg aggtattata cctttttctg gatgaaccca tctaaccaat gcttctgcac
    10921 caacaatttt atttgcatta agattatact taggttgtaa atatacatgg aattgcccat
    10981 tttcaagtgc gtattccatt tccctttcca tctgtttttc ttcaagtgca tcatttctta
    11041 tagtatcatc ataaaatata tagttattga tactttgttt ctttactttt ttgtgagcat
    11101 atcctgccat ttcacacgca tttgtaattg gaatatgatt atccaaaatt ctataaaccc
    11161 caaaacatgg tactaacata tattcaaatt tttttctatt taagctattt ttaatcttat
    11221 aaattacctt atgaatatct ctatcagagt aactctcata aaaaatgcaa aatacatctg
    11281 catacatacg acaacatata ccattagaac caatgttttt ttgaatcaac tctgcaacat
    11341 atattaaagc tttatctcct cctctaactc cataaatgtc attaattacc ttgaacttat
    11401 ctatatccat cattataatt gcataacgct tgtcaggata tcttttaagc attaaactta
    11461 tttgttcaca aaaactttca taattgtata gtcctgttaa tgtatctttt tggtttttat
    11521 gtagttgcat aagataatct atatttttta tagcaactaa aatatgtgtt tgtttctcac
    11581 ctaacttatt tttactacta atctttaaca tataaattaa tgcccaatag tactttttat
    11641 ctattcctaa acgtctatac tcaaaattta gttctaaatc gtcttgcttg ctattattaa
    11701 gactatcaag agattttaga aggtggttat cattaaataa tattatatca tcaggatgaa
    11761 ttaaatgttt ttttatgtat gaaaaagttt tttcaagatt ttcctttata ggaaaactaa
    11821 aaatattgct ctctgattga taaattggag taattaaatt cgtatcaatg caaatagata
    11881 aaattatatc atatgtatta taagctaatt tttcataagc cttagaatac ccttttatca
    11941 ttaaatcatc aaaataaacc ttatcaacta actctttatg tttaaataaa atattattcg
    12001 tcgaatcagc taagtcacta attactttca tttcattatt tctaagtttg ttcttattat
    12061 aatacctctt cttatcttgg tacattctct catcagcaac tttaattagc ttatgtatat
    12121 caatattatc gtcactccaa atactgccca ctgctacact atatatcatc tcatccttaa
    12181 attcttcact taaccaactc actaattctt caaatagttg ttttgatata tcttcacata
    12241 atacaacaaa ctcatctcct ccaatacggt aaacattata agaatgaaag tattttttta
    12301 ttattacaca tgctgacaca atagcagaat cgccattagc atgtccaaag ttatcattaa
    12361 tatcttttaa cccatttata tctacaaata caactcctat ggatttaact tttccttttt
    12421 gaaaattatc tatatactta atatatttat ttctattata tgcaccagtc aagaagtcat
    12481 agtagctcat ataatttaat ttattttcta cttttctttt tttcatctca gttaataaaa
    12541 aataagatag ggatgtcaaa agagaggtat ctccaacagc tttatgtgga ttatcaactc
    12601 caataaaccc tactacttta tcatcttgtt ttagtgaaga tgtcattaaa ctttcaattt
    12661 tttgaggttt taaaatttca tattctattg gctgttcttc ttttatattt tctatatcat
    12721 caattattac aaaacctttt tgttcgaata ataaaaacca attctttaat tcattaatat
    12781 caagatttgt tagtgcctgt ttttgttcat caacaccttc attgcaccat tcatatgtat
    12841 tagttacttt ttttctatct tctgatactt caaaaacata tgctctatca gctttatgaa
    12901 atttaccaat attggttaat acaagattta cagcatcaga aaaattagat tctgttataa
    12961 gatgtttaat acatccaact aataactcct ttatctctaa tttttctgct attgacttac
    13021 ttatatcttc cttttctgta atatcaagag ctatttctaa tcgtgcatgt ttgccctccc
    13081 atttaattaa cttgtccttt aatataaaat gtctatttgt tcgagtattc gtattttccc
    13141 atatgtaata ttcatcttct tttaaaatag agtttgtaca aaattcgcag ggagaatctt
    13201 tcccctgtaa tgctttataa cattttaaat gggatatatc attaagtccc attgcctttt
    13261 tcccaatttc attaatatat aaaagttcat atgtatctaa agaagaaaca tatacaattt
    13321 catccaactc atctattaaa tttattacat ccatttatat atatctcctt ttattcattt
    13381 tccagattga atttttaaag cttttaaaat tttgctatat atttggcaat gatattatat
    13441 ttctttatgt tttaaaatag taccttaatg aggttctatt ttttagtaaa tagaattatt
    13501 attgtgtcaa tcgtacaata gagttttgta atacctaatt taatatattt ataattaatt
    13561 atctcattat gtgtttataa ttaattctca tataaatgac aaatctccac ctcaattaag
    13621 tattccaatc atatatgaag ataatacttc attcatttaa aaatataaaa ctattatata
    13681 ttaaaatttc ttcaataata aattatctag attgttactt tgatatatta tataatatca
    13741 caaaggctga tataaaaaga caaaaagggc aattttttta tatcaaaaaa aaatgttaaa
    13801 ttgatattat ccatatataa attgatatta tccatataga tgctatattt aacaattcta
    13861 cttacttccc ttacacttat tagcatatag ttattaagag tttgttgcaa aacaacactt
    13921 agcacacttt aaaaagtgtg gtctaataag taatcaacga agaagacata ataaagcata
    13981 aaatatgata tacttttcca cgccatcaaa actgaggatt ttattattaa tatatgctaa
    14041 aatatgctta ataaattaaa ttttaatggg agaatagtta tgaaaatatt tagtttcttg
    14101 ttgtttgtga taggaatatg tttatgtatt gctggattta taggacatta cccagtattt
    14161 gttggtacaa cattgagttt tcttgcaatt gcaatgatag ctgtaagaca agtcaaaact
    14221 aagaaaaaaa tgaaataggc aaatcctaac tttacaagtt aatattacac aagtaattag
    14281 tctaaaattg gatacttttt ctattacaga aatacatcat ccactttgta ttttcttaaa
    14341 aaaataaaat agctatgtaa attcaagatt agatagatta actctctatc catttcattg
    14401 taaaatatac atagctattt gtattcgatt taaaatactt agattttctt acataacaaa
    14461 taatttctat ctgattatta ctcatattca tttagtaacc actcagcaac taatcattta
    14521 gtatctattc atccgccata cggtaaccta ccccaacctc agtcaaaata tattctggct
    14581 gagcaggatt cttttcaatt ttccttctta tacttgccat aaaaactcta agcgactgat
    14641 tttcactttc atagtaattt ccccaaactt tatctattat aaacttatgg gttaagactc
    14701 ttcctgaata cttagacatc aactcaataa tcttatattc gattggagtt aaatgtattt
    14761 cttcaccatt aattgacaca atatgatttt cataatctat ctttaggttt ttaacttcaa
    14821 aagactttgg agcatcatta tttatattat cctgttgagc tttatttcta agagccacac
    14881 gtactctagc caaaagctct gttacactaa aaggtttagt taaataatcg tctgctcctc
    14941 catctaaagc ttccacctta tccctctcat gctctctcgc tgaaacaaca attattttat
    15001 tagtctttgc acatgcccta actcgtccta ttacctcaat cccatcaata tctggtagtc
    15061 ctaaatccaa aattatcaaa tctgggtcaa gagaatgtat aagtgatata gctgttcctc
    15121 cattatctgt ctctacacac ctataatttt gagtttcaag agatactttt ataaatttac
    15181 aaattggctt atcatcttct acaattagaa ctaatggctt tatttccact tgtctttccc
    15241 ctcctcatca tacataggta tgctaaatct aaatgtagct cctccagaag gattattaaa
    15301 ggcgacaatt tctccaccat gagcttctac tatagattta catatctcca atccaagacc
    15361 aatacctctt ctttcaagat ttctagactt attcttagta taaaacctat taaaaatatt
    15421 cccgatatct tcatttggta ttccttttcc attatctgca acttcaaata gtacttgatt
    15481 tgactctttt tttacagtta gttctatctt agaattttta ggtgtatgcc ttatagcatt
    15541 atcaattaga tttactaaca cttgttctat taataataca tccacatgaa cgattattat
    15601 ctcatctgga atatttgttt ttatgtctct actattggca aactttttga ctcttaatat
    15661 agattcagct attatttcat caacaacttc tggtcgtttt tcaatatcca attttccttc
    15721 atctattctg gtcatactaa gtatattctc aacagaatga gttagccaac tcgtatcttc
    15781 atatatattt ttcaataatt ctactctagt ttctttatct atcacatcat cattttcaag
    15841 tattgttgaa gatgaaccta atatacttgt aagtggagtt cttaaatcat gagaaattga
    15901 cctaagcaag tttcctctta atctctctct ctctgcctca agatttgctt ttttattttt
    15961 ctcaaataaa ttttctctat caattgccaa cgcaacctgt gttgaaactg cttctaatag
    16021 tatcttttca ttttctgtaa taatatctat cttaaaacac gcaacaccta taacaccaag
    16081 aacatttgat tcttgacctt ttatagggaa ataatatgca ctgctgtttt tacaatattt
    16141 tgtcccaatt ccaactgggt tacctgtcct aaaagcttct tttatagctt ctttttctaa
    16201 tactgaatgg aatatattct cacttccatc attgttacaa atataaatac ttggcttacc
    16261 tagattatta ttctcatctg caatagtcac tattacagtt ctatttaata tatttactaa
    16321 attagaccca caaaattcaa taatttgatt tttatttttt gctttaagca acgttttgtt
    16381 attctcatag agcaatctta tcattttttc tctcttatga gaaagtctca cttctctttt
    16441 tatcctagaa gttaaagtac ttgttataat tgctgccatt agcataatac aaaaggtcat
    16501 aggataatct tttctataag ctaaaaaagt atataatgga tgcgtaaaga aaaaattaaa
    16561 tgatagcact cctattactg aagaaagtat tccaaaaaaa tatccatagg ttatacttgt
    16621 agcaaatagt acacctaata tgtatactag tataatatta gattcattaa aacctacctt
    16681 ttcaaaaact actgatattc cagtagcaat tgtcataacc aatatagttt ttatgaagtc
    16741 cttaataatt atctttttac tgagatagcc atcttcatca taatcatact cttttaagtc
    16801 ttcatgttgt agtcgttcct ttttcattta ctacaattcc tccttattgt tactgtttaa
    16861 attggctcta ttttatataa tacaatttta tcataataaa ttcaatcaat acatttaatt
    16921 aatgtactta aattactaaa aggtgctgtc ttaaaatgaa aataaatctc tatttcaaaa
    16981 cagcacctat atatttaaat tactaattta gtttttacat acttaaatca atagttttat
    17041 tcaatttcat aaattataat acttcaatta taaaacattt ctatccaaca agcataaata
    17101 taaagtgggc acatatacct gcacataaac cacctatagt atgcgaaagt atcgctttac
    17161 cagccaatgg tcttgcatct aaagcatcca tcatacctat gtgtgtactt aaatatccac
    17221 tccaacacat acccattgct gtgaatactg ctatatcatt aggagttata gcgcctgatt
    17281 ttataaactc tggtactaaa gatattgctg ctccaacagc tcctaatgca gttacaggga
    17341 atgctattgc ctctggtgat gtgaatccaa ataatggttc tattatgaag ctaatcttat
    17401 caccaatagc aggtaataat tttatacctt catatgcagc tcctgtgtaa acagcttgac
    17461 ctgtagcagg gtctgtagat ggtccaaaag ttaataacat aactaatgta caaactacta
    17521 aaactcctgg tattatagcc atacccattt caacacctaa cttaccacct tcaagtatag
    17581 catccaatgt tctttggaat acattaccat ctcttatttc tctaaactct tcaagttttg
    17641 cacctttatc atttatttgc ttttcagtat catttttagg gtcataatta taatactttt
    17701 tagtaaaagt caacataatt ctaacactaa taatactacc tattattgcc gcaacattac
    17761 ctattatagc tggtagtaca tattctttac cttgagctat cataaaagtt gttacaataa
    17821 gtcccatacc aaaggctgtt cctaaattac ataaagcagg tacttgatac ttcttaaaat
    17881 attgtgtaaa tgttttgtct ttagcaaatg gtataatagc tggattatca gataaatatg
    17941 ttgcaactac tcctgctata ctagcaccag gtaagcccca tataggtctc ataagaccct
    18001 taaatatctt attaactaaa gatattactc caaactctga aagcaatgca ctaagtgcac
    18061 ctgcaagaac tgccatagcc attattaaaa atactgtgtc taataaaagc gcatgtgctg
    18121 tactcattat tactttaaac atcattcctg cacccatgat gcttcctaca tatccaaatc
    18181 ctaccgccaa tagtacaata aatacgaagg tttccatact tactgccttg actcttctct
    18241 tatccgacag cttttccatc ttgaaatctt ccccctttta attttaaatt aaggttccta
    18301 acattggtac aaattttttg gtacaaactg agttctaaaa cttacactcg taatgcaagt
    18361 tgtcagtttt ttgaaataaa aaaaaattac gtttatagaa taagaatatc ctctttaatt
    18421 tggatactct tattctattt tatgttatta tattatcatc cttttgttta aaagtcaatt
    18481 ttttctattt ttgttgttta attttattta tactcttttt atatttttca ttttctttta
    18541 tctttgtaag tactccatat aagttgactt ttctaaatac agtagttata aataccaatc
    18601 ctaatgcaat tgaacttgca cacgataatg tactcacccc atattctaca gcttttatat
    18661 aattattggt caaaacagaa tacatagtat tatatattcc ataacctggt actaatatta
    18721 ttaaacagca aacacttaat gtagttgctg gagtcatata aattcttgca cataactcac
    18781 aatatatcgc aaaacaaact gtagctataa aaaaacataa gttttctgtt actcctatat
    18841 ttaaaagaaa tttatatgaa aaccaagcta tacttccacc tattcctgct agtattaagt
    18901 tttttccttt catattaaat agcactccaa agcttaaggc agtaaaaaaa gctgctatta
    18961 cttctataaa caaatgcact tatatccccc ctaacttgaa gtacaatcca agtaccaaga
    19021 acattcctac tgctatagat acacctacaa aaaatacttc tgctaatttt gcaagaccag
    19081 aaatcaactg tccatctaac aaatctctta atgcattagt caaagcaagc ccaggaacta
    19141 agagcattat tgctcctgct attaaatggt ctaacgtctt tatgaatccg agctttaaaa
    19201 ttaaaattga acaagaagct ataataactg ctccaattgc atttacaaaa aagtcattaa
    19261 gggaagattt acttaaatac ataataacaa acttagtcag aatcccaaca gtaaatgcag
    19321 ctataaattc ttccacattt cctccaaata atatcgaaaa agttgctgct gcaatacctg
    19381 caaaaaatat tgttattaaa tctgagtaat agttatcttc acttatttca tctaaaattt
    19441 tctcacataa ttcaatatct aaattattta tacttattgc tcttgataat gagttaactt
    19501 catgaaccaa gtttaggtct actcttcttg agcttattct tttaattgct gaatgtaatt
    19561 tcccatctac aaatacagat gccattacag ctgttggagt tgcaaataca tctacgtggt
    19621 ctattccaaa agattgacat attctagcta tcgtttcttc aactctatag gtttctcctc
    19681 cactttgcaa catagcttta cctgcatttg aggaaaagtt taaaattcta tctatatcca
    19741 caaaacatat ctcccattct tttatagtat tattcatatt ttaatacaag atattattta
    19801 tttaaactat tttaaataat tcatatccaa tatttacatt ttatgaaaaa acttgttttt
    19861 tatgcttgtc aaaatcaaat tttaagcttg tttacaccat aaaatggctg aattaatatt
    19921 tgacaaacac tttcaaccat ttatataacc ttctaaattg tataactttt tactacaaat
    19981 aaaattatat ctcttttaat tctttctctt gagtatttag cttatagtaa cttcatattt
    20041 atatatgtac aatataaaac attattttta tgatacaaaa tataatttgt aaatgtatat
    20101 atgtattaac tgtgtatata taaattattc ttgaccttat cagttttttc atatatcttc
    20161 atctatacca accatatatt aataaatgat attacataga cttaagtata ttaaaaaact
    20221 taaatttagt tatttaacaa cataatactc taatttaatc tataaaaatc catacttaat
    20281 ataatgcaac tcttatttta acaccatcta ttacacaaaa caacatatta aaaagtaaaa
    20341 tacaaaatat ccactataaa ccttatttta atactgaata tatattataa tatatttaat
    20401 gtagcttaaa tatagtttaa acataaagaa aggaaacggt atatgctaag aaaagataaa
    20461 gttctattat ctctacataa attatgtgaa aatatgactt taaaagacct tcaatcagat
    20521 aaaacaggat tcactacaat agaaattgct aaccattcat ctcttgatag aagtaatacc
    20581 agtaaagaat taaataacct atacaatgaa aaaaaagttc tgaaaatatc tggtaagcca
    20641 attttatttc tacatgctag tattatagaa aatttaattg gccgtacttt gaacagtgat
    20701 gaattttcta taaataattg taatgagcta cttaaatcaa gtaaaaatga ctcagaagat
    20761 attttttcaa cattacttgg acatgattct agtcttaaac tacctataga aaaagctaaa
    20821 tctgctatac tctatcctcc aaaaggtctt aatacgttac taataggacc tactggtgtt
    20881 ggaaaaagta catttgctga aacaatgtac aaatatgctg tagaaagtaa aatcttttct
    20941 tctgaaagca aattcgtagt atttaactgt gctgaatatg cagaaaatgc taacttatta
    21001 ttaagtaatt tatttggtta tgtcaaaggt tcttttactg gtgctaataa ggataaattc
    21061 ggcatagttg cacaggctga tggaggcata ttattcttag atgaaataca cagattgcca
    21121 ccagaaggtc aagagatgct ttttcttttg atggacaaag gcatatacag acgattagga
    21181 gaatctgaca ttatgcacaa agctaatgtc tttattgtgt gtgccacaac agaagatata
    21241 cattcttctc tgcttgggac ttttttaaga agaatccctg ttacaatcaa cctacctgct
    21301 ttacaggata ggtctttaaa agaaagatta tctcttatta aatacttctt tattatagaa
    21361 tcaaaataca ccaatgcaac tataaaagtt cacaaagatg taattaaatc attactcctt
    21421 tatgattgtt ttggtaatat aggtcagctt aaatctgaca tacaacttat gagtgcaaga
    21481 gccttcttaa attacaaaac tggacttaag gataatatag aaatagattt atcccttgtt
    21541 cctgattaca tctatattgg tcttttaaaa ttgaatgaaa atcgtagtaa tctaaataaa
    21601 ttatcagaac ttgattatat gcttttttat gaatttaatg gtaataactt agaagttgaa
    21661 tatacaaaag aactatctaa atacaatata gatacatcaa acaatggatt taaatactct
    21721 acagagattc cttatctgga aaatgatata aggagtatgt ttataaatta catggaaaag
    21781 tactctacaa aattactttc gatatcttca gtaactcatt ctgattatac aagtaatgag
    21841 atttttaaag taatcaatcc aaagatttac tatgctgttg aacattcttt aaaagaggct
    21901 gaaaaaatat cgagaaacaa atacacaaaa caaacttata ttgctctttc aatgcatata
    21961 agtgcactta tagaaaatat aaatcaagac acatataatc ctaagtacaa tttatataat
    22021 gataaaattt tcttagataa agatttaaat atagctaaaa tagttttaag tataattgaa
    22081 aatgacttac aaacatcttt acctaaagat gaagtaaaat ttatatccat gtttctaaat
    22141 agcatgtatc ttgaaccaaa caatcaatct gaaagtatag gagtcataat acttgctcat
    22201 ggaaattcaa cagcaagcag tatgtgtgat gtagcaaatt cattgcttgg tacaaaccat
    22261 tgtaatgcaa ttgacatgcc tcttgatgta aaagtaagta ccatacttga taaaactata
    22321 gagttggtaa aagaatgtga cgaaggtaag ggtgtacttc ttttaacaga tatgggctct
    22381 cttatagctt ttggagaact tatttcagaa aaaacaaaaa tcaaaactcg ctcaattgaa
    22441 atggtatcta ctccaatagt tttagaagca gtaagaaaaa gtacactacc agaaatgact
    22501 ttggatgaat tagctaattc cttaagctct ttaaatccat atattggacg agcagcaatc
    22561 gattcaacag aaataaacta tcctgaaaag tcaagagagt atgtaataat aactacttgt
    22621 ataacaggat atggttcagc tatgaaagta gcagacttta taaattcttc acttaaaaat
    22681 attagtaaat acaatattaa tttagttcct cataatatag aaagttttaa aatgtataag
    22741 aataaattta gtaatactaa aattctggca gttgttggaa gtgtagattt taatgtagcc
    22801 aacacccctt ttatacacat agaggaacta ctaaatggat atggcctaga tatgctaaac
    22861 aaaataatag aaggtacatt caatgccaat agtattacaa atataaaaga aacaaatcta
    22921 tcatacaaca gtttaataat aaactttatg acccaaaact tagagctttt ggactctgtt
    22981 aaattattca actatgtcaa ttcttcctta tttacaataa tagaattaat agatgaaaac
    23041 ttaaagagca aatttaagct aggatatata ctacattgct cttttatgat agaaagatgt
    23101 tttaaaaata caacttttcc atataataac attactgaat taattagtaa aaatcatgaa
    23161 ctatatagtt taatacgtga agcctttaag ccaacagaag cttattttaa tattttgata
    23221 ccagacgaag aaattgctta tataatagac atgattaatg agtatctaaa taaataaaat
    23281 ttatagcact tattttgtgc atattaatta atatatgaaa tggaggacga gattttgaaa
    23341 ggaaaaataa aacagttaac aatacttgca ttaattttta tatttataac tcctgtattt
    23401 gcatttgcag atactccacc agtaccaaat tcttcaagag cagcactact tatagaccag
    23461 gaaactaaaa gaatattatt tgaaaaaaac attgatgaaa aaatgccatt agcgagttta
    23521 agtaaaatga tgaccttttt attggcaata gaagcagttg ataaaaatca agttaaagag
    23581 actgatatgg tgaaaataga caagtctaca gcttcagtag gaggttctac ttgtaaactt
    23641 aaagatggtg atgaaatatc tctaggagag cttatgcagg gtctaatgct agtatctgga
    23701 aatgatgctg ctatagccat agctaaacat attggtaaaa ctgaaaagaa ttttgtaaat
    23761 atgatgaaca aaaaagcaga agaaatagga atgattgata cgtattattt taatccaaat
    23821 gggcttccta tatatactga cccagaacat aaagaacctc ctattgaaaa tatgtcaact
    23881 gctcacgata tagttacact tggaaagtat atgtacgacc attatgaaaa tcaagttaca
    23941 agaataacca ctatgcaagt atataatgat actaaaaagg attttacaca ttataacaca
    24001 aatcctctgc ttgtttcagt acctggtgtt gatggaataa agacaggata tacagataat
    24061 gctggatatt gccttgcatt ttctatgatg gtacctaaag atgctaaaaa cgaaagaaat
    24121 catagattga taggtgttgt acttggtgat ggtaacaaga aaaatagaat ttcttcttcc
    24181 gctactttat tgaaatatgg taaagataat ttccattcta aaaagatagc acataagggt
    24241 gatattatag agactccttg tgttgatggt attgatgatt ttaagataac agttaaggtt
    24301 gataaagatt tgtatggtgt tgtttcagat aacgaaaata tcaatcctaa ggtagtgttt
    24361 aaaaatatga attatcctat ccataaggga gatattgttg gtgttgcaaa gtattataat
    24421 gactctggta aatttgttgg aagtgttgat gtaaaaagtg aaagtaatat aggatgtatt
    24481 cctttgaagg ataagattaa gattaaggtt gctaagataa ataaaaaact tgagattaaa
    24541 aattctgttt gttttaaagc atagtaaagc atattattaa aagggtatgt taaatttata
    24601 aagactccac tttgtggagt ctttttgttt tttacaaaaa attaaaatag tggcaacaat
    24661 tgaggatgtg agggagagtt gccactattt tgtatagggt attatattat gtttttaagc
    24721 tagttttact atcttctatg tttatataat aactcttgtg tgtgaattta attgataatt
    24781 aatatgaaaa ttatgtgaag ttttgattta tttgtaatat attctactac tattataatt
    24841 tttctactgg atatactaat catttgttta acattatctg agattgattt gcaaactttt
    24901 atataactaa attagttata taacttatag attccattaa ataccctaat aatcatatat
    24961 gtacatacaa agtcatgctt atgcaaaatc tgcagtttca actaacttat tatataaacc
    25021 atctttatgt agaagttcag catcatttcc agattctatt actctacctt tatctaatac
    25081 atatatatta tcagcactct taattgttga aagtctatga gctattataa taactatctt
    25141 atcttgtatg tactctttca agttgttcaa tatctctttt tgtgaaatat tatctagagc
    25201 tgaagttgct tcgtccagaa taagtacctt tttattactt actaaagctc tagctattga
    25261 tatacgttgt ttttgacctc ctgatatttt gacacctttt tctcctacta cttcgtccaa
    25321 tcccttatct agatttttta taaaatcatc taaacaagta acttctaata tctcatctaa
    25381 ttctttatca cttattttat tatttaatac tatattattt tttattgtgt catcaaatac
    25441 aaattgatct tgagttacta tatcaatatt atttcttagt gattttaaat taatatcctt
    25501 tatattaaca ccatctatta atatatcacc ttcatttata tcccaaagtc ggtatattag
    25561 atttgttatt gttgatttgc cacatccact tccaccaaca attcctgtta cttccccttt
    25621 ctcaaattgt aaatttatat catttaaaac aaggtttaaa tcctcaccat atctaaaaga
    25681 tacattttta aaatctattt tcttaatttc actgttatct attctatatc cattattatc
    25741 aactttaata tctatctttt catctattac tgaaaatact ctattgatag acacttttga
    25801 ttgtgctatt ctagtattcg cccttactat agctatacat ggtccaatta acattcctgt
    25861 atattgttga aatgctatta actcccccat cgtcatctta ttatttatta ttttatatcc
    25921 cccaattcca tatattaata ttgttatcaa tcctgttagt atttgagata ttgctatatt
    25981 actactcata atgatgttca aattcaaatc tttttttatc aaatctctct ctctctttaa
    26041 atacttatta aagaaattct tttttgaatt taatataaca atatccataa tattagatac
    26101 gtactcttga atcaaatttg ataaactgcc tgaatcactt cttatttcag aaattttctt
    26161 agatataagt tttgtcattt tattttgaga aaatgttaat atcaactgta aaaataaaac
    26221 tactaaaaat aaatcaaact gcaaattaat taagaaaatt aacgatatta tagctgtaaa
    26281 cacatttaca ataatatcaa atataatctc tgctccaaag ttttctatca taaatatatc
    26341 actttccata atggataaaa tattacctgt tttcttactt gaataatatc taccagaaag
    26401 ttttgatata tggttaagca actttacttt tagatttata gtaactttat tttttattga
    26461 agtatataaa tatgataata taacatcaaa tgtagctgat aatatcctta taaaaatata
    26521 tatcaatatt aaatttataa gcagaaaaat atttttattt cctattccat catctattat
    26581 acatctaatt agttgaggta ttacaatatt tacgccagca attataaaaa ccattataaa
    26641 tgatactatc aacttagtct tattcttata tactattata tttagtattc taaataaatt
    26701 attattaata gtttttttca tctatttttc catcttttat agttataatt ctatcagctg
    26761 tctttgcaat attatcatta tgagttatca taactagtgt ttgtttgtac tctttaacac
    26821 accattcaag catattcata acttcatttg tagtcttact atctaagttg ccagtaggtt
    26881 catctgcaaa tattactgac ggtctattgg ctaaagctct agctattgca actctttgtt
    26941 gttgacctcc tgatagttca ttcggatatt tctttatttg tttttcaatc cccaacttat
    27001 taattaatct cataacatat tctttatctt cttttttccc atcaagcaac acaggaagta
    27061 ctatattatc atatacattt ataactggta ttaaattgta actttgaaat atgaagccaa
    27121 attcctgtct tcttatttca gacagcttat catcatttaa tgtatataaa tctacatcat
    27181 ctaagtacac attccctaaa gttggcttgt ctaagcctgc aacacagtga agtagtgtac
    27241 ttttaccaga accacttgca cctacaatag ctgtaaattt atgtggctct atatctaaat
    27301 caataccatc aagagctcta acttcattct catattttcc atatattttt tttaaatctt
    27361 ttaccttaag tattttcata acgtatctcc attcccattt aaaaaacata tatttttaaa
    27421 ttaaattttc gtaaataaga tctactgcca tatctaaatt ctctaattga tataagtata
    27481 cttctttgta gtcaatacta atattaaact ctttatttaa aatagttaaa aaattatatg
    27541 cattataatt tccaatacat gtaaaattgt taaataaaga atattcactt acaaattcaa
    27601 agtctatttt tattttagaa aagtttctat ttatcacatt tattattttc cttgctatat
    27661 ccaatcttat caaattagat ttataacttg gagtgttcaa gttttctatt tttaaatctt
    27721 ctttactttt gcttttttga ataagtattt cttccaattc attaaactta atatatggat
    27781 tgttttttaa tatatttgca acaatgccag ttgccactgc acaagcctta ctatttttac
    27841 caaaatgagt tactttatct ttaaatttaa taaaattatc ttttgaattt gcatacattt
    27901 gaatcttatt ttctggttcg tatatatatt gattattttt atatatattt tcattaccta
    27961 ctacaccaat aacactttca aatatagtag gaatactttc tttatcttca tatttattat
    28021 gttttgaaca aataataaat ctaccttgac tatttaaaat attacaaacc ttttctaatt
    28081 ctttttcata catactatta atagatgcag ctgaaatgtt tattatctgt atatccgaca
    28141 tagtcaactt gctcagtgta ctaagaagat ttaaagaact tgtttttcca gaatcattaa
    28201 atatattaat aggataaatt tgggtatttt tacttatatt taatatggtt ttcgatacaa
    28261 aagttccatg tccatttatg tcatttattt ccttcccccc caaatctttc aattgaaaat
    28321 ttgaatcata aacataattt ccattaatat catcatcatt tacatctaat cctgtgtcta
    28381 agactgctat tcttacttta gtcaattaat aatcacctct aaaataaatt accttgaata
    28441 taaaactaat ccttaccata atataggttc caagatgttt ttcatttttt tacaatttgc
    28501 atcaaacatt tttttgttat tttttaaagt atatattttc gatggacaag ttgtacaaaa
    28561 taaatttact ttacattctt tacaactctc aatatttact ggttttaatt tatcaaatat
    28621 attaaatata tccatttctc tattaagaat cctctctaat atgttttcat taaattctaa
    28681 tatattgaat atctttaaat ccttatactc taagtttgga cacggatata catctccttt
    28741 atgatctata cttatctgat taacacctgc tttacacata tttgaacata aactactttc
    28801 atccacataa tcatctttgc aaatgtaatc aatatcaagt ttatcattaa gatatttgct
    28861 atagttctca ttacctctac caatattcat aaaatttctt attataggcg ttgtacctaa
    28921 atcttcattt aatttattga atttctctat gtctttctca ttattctttc ctaccaccat
    28981 agacaaacct acgttctcga atttttttag ttttataagt ctaatcacat ttaatacttt
    29041 acagaatact cctttacctc ttacttcact acaacttaat tcatcataac catctaaact
    29101 aatttctatt gcatataaac cattaattat atcatcaatc tgtttttctt taattaaagt
    29161 tgcatttgta gacagaatta ttttaccttt aaatttttct tttatataat ccaacagctc
    29221 taagaaatca tttctcatca atggctctcc acctgtaatg gttaatatcc ttggatttaa
    29281 tttaactata ttatctatta cttttcttac ttgagatgta tctaaataat cgttattatc
    29341 tattttagaa tctacacagc aataatcaca acttagatta catctattag ttggagaaaa
    29401 tgaaattttg tttattctct ctagtctaaa ttcacttaga atagaatcac ttagaagtcc
    29461 tatatgctct aacttatcta taactcgttc ataatatttt ttatcttcat tttcttggaa
    29521 tacatcatac aaattaccaa gttgtaatgc attatctact gaatacttta gaacttcata
    29581 acattctatt ggaattttaa cccatcttcc atttacttta ttactaagaa taacttgatt
    29641 attatttatt attttttcac atatacaatc taaaaaatat ttgtttgagc tattcattta
    29701 ttttatttcc ttcctatata tttaaattag aaatttcatt aattttcttt tataccactt
    29761 actatactaa gtttatttat cattttatta gaaaaataaa ctgcaatgac acaaacaaaa
    29821 acagatatag atccaaatat tagaatttca tatattggaa tttcaaattt aatagagtta
    29881 tttataccat atacctgttg attaagtttg ttagtttcag aaactatttt ataataattg
    29941 aaactagata taattccagc tacaatcata cttaataccc catatatcat attttctttt
    30001 attaacattc tttttacatt ttttttggac atccctattg ctctaaatgt acatatctca
    30061 ctcaacctca caattatatt tgatctaact ataaaaataa tgtttataga tgatattaat
    30121 aaaactccaa atacacaaat cattgctaat ttatatgagg atttattata atggtctcca
    30181 aaatgattac gtacatatct tccctctata tctgaaaatc cacttttttc tattatatta
    30241 tttatttctt ttattacttt tttatctgtt tcttttttta tcttcacaaa aactttatta
    30301 taatcactta tccctgttaa ttcttttaaa tccttttcat taaatataat ttgaattcca
    30361 cctgattgag attctccatc ttgctctgat atatagctat ttttcattat tccagcgatt
    30421 cttacttttg tatttatata tttatactct ccatctttat atacaggaat ttttatatct
    30481 attaagtctc ctattttagg tgattttata atctgagtat tatttgacaa tttaattcta
    30541 gaataaaaat tatttaccaa taaaacattt ttataatccc tgtctgtata actataaaca
    30601 ttttcgcctt tatctatata tttatctata ttatttaata gctctttatt gtaacctcga
    30661 attaaaactg tagactcatt attattattt atttcagttc tttttagctc atctaaataa
    30721 tcttcttcta agtcagcatt tcttagatat ccttttaaat aaatattggg ttcaacatct
    30781 gttacatctt caatagcttt tatttcattt aataaagaat tatcatattt agaaaatgta
    30841 taatttgcat ttattggtga atatcccaaa taaatatcaa aattgccata tgatttactt
    30901 gttatattag ctgaatctga ctcttttcca tatatgaatt ttgtagaagt tagtctgcct
    30961 ataaataaaa ccccacatag tgatatcgta attattatta ttattgatgt aaccttattc
    31021 ctaaatatat ttttaaaagc tatctcattt ataattccaa caactttttt atgtgatttt
    31081 aacaacaaaa gcctaggaat ataatttaac ttatctgtct tttttatagc atcaattata
    31141 gataaatttc cagctttata tattggtact acagaggcta atataactgc tataattgac
    31201 atgatgatag attcaattat agagtttgta tttatagtta attctgcact tttatcatat
    31261 actatcatga gacctaagta agaaaaaact atacctccaa gtaaaccaat tactatacct
    31321 acagataaaa ctacaatact ctgaataaaa aatacctgtc taatatttct cttgctagct
    31381 ccaacagctc taagtaaacc aatttgcttg gtcatatcta gtattattat gttaaacata
    31441 ttgtatatta ctaatgttgc acataataaa gggagtaagc tttgctttat tgaataagtt
    31501 gttttttttg aaatattttt catatacata gacatactta cttctgtatt tggataaaat
    31561 tgattacttg ttaacttata ttttctccct agattcacta ctttttgaga atctaaatct
    31621 tttgtattca attttaatgt acccatatga gttattaatt catatggaac aatatctttg
    31681 ccattgtttg aatatgtaaa agctcttacc ttatatttat ttaaattttc atagtattca
    31741 tcagatttac ttaaaatacc aactatttta aattgcttat ctttactata aatagaattt
    31801 tcattatttt tatttatatg ctcattaaca atctgaaaat caatagtttg atttaatttg
    31861 acatctaatt ccattttgtc taaaactttt tgttctatta ctaattcatt agaagttcta
    31921 ggctctctac ctttaactaa cttatatcta gtagaatcta aaaaatcttt attgtaagag
    31981 cataaattta gagatgctcc attatcttta cttataattt ctccaaattt aagtacatca
    32041 ttgaattctt ttatatcttc ttcaccatta agttctttta ctatatcact atttatatct
    32101 tcataaaaag catgataatc tcccgagttt ttgtatgcat gatttatatc tgattgtcct
    32161 tgagattcat taataacact taatgaaaaa actagcataa cagcaatccc tatactaagt
    32221 attaatgata aagtttttcc tttttgtctc tttaaatatg acaccgcaag tttaatattt
    32281 atcataatca gaatcctcct tattcatatt tacaagaaaa actctataac ctaaaatagt
    32341 aggttataga gcactattca ttaaatacta actaactcgt tgtgctagtt aaatttaatt
    32401 attaaaaata ctagagttga gaaaatcaca tttcatttgt aaaaaactac atctagtact
    32461 ttcaacatat ttaatagact gattagtatt ttttaataag ctactgttcc ttttggagtt
    32521 gatcctgcac aatccaccat acagtctatt tgtgaaatta ttccatgctt atcataatac
    32581 catttacatg gatctgcttg cacctcaaca tcttgaagtt ctattttttc ttcgattttt
    32641 tttatatgta tcataataca tatccccctt tacttttgtt ttatatttat tataactagt
    32701 taaactagat tataaactac atagtttttt tgttgtttcc acataaactc tttctaatcc
    32761 acaataaatt ggtactgcac tttggaattt acctgttacc tttttattga taagtttaca
    32821 tctggttgac atacaataat caagtctagt acatccatca cactcttcat tcttagatat
    32881 ataaattttt ataaggttct taattttttt atcatctaat ccatcatata tatttcctag
    32941 taaaatatct ttatctccta cacatactgt acatggatat acatctcctc tagtatctat
    33001 tgcataacta tttatccctc caaaacaatc tccttttttc ttatttataa attgtttatc
    33061 taaaatagat atatctatat caggataatc ctttttaata ttcataattt tactaatttg
    33121 ttcatttaat attttaatat gagaattatc ccaattttta tccataaaat ctgaaattgg
    33181 tgcgatattt ttaaaccctt gttttgctat atcatataca ctctcataaa gatacttaac
    33241 agtatttgag ttatatgtca ttcttgccct aacatatata cctttatcta taagtctttt
    33301 tccattttcc attacttttt cataagtacc tactccgtta gcatctattc tatttatatc
    33361 atgtatattc ttttttccat caatacttat cgatatgttt tttacattct ttgctataaa
    33421 ctctattatt tcatcattta taatagttcc atttgtagtt atatcaaata atatcttttt
    33481 gtcactcaat ctatctacta aatccctatg tatatattta atcaaatcaa aatttataaa
    33541 tggttctcct ccatgtaaaa caattgttgt ataatcacta ttactttcta attctttatt
    33601 acaaataaaa tctattgttt tatcagcagt ttcataactc atattttctt tttttttatc
    33661 attaccttca taacaatatg tacatctcaa attgcagtct tcagttacaa atatagtaaa
    33721 tttcaattaa ctttttcatc tccccataca gctgtattta aattgtgttt catcatacta
    33781 caccatctat ccaattccaa ttcattatcc ttaactgaat ctaatatcgc tgggcaatgc
    33841 caacaaaaaa tatttacatc acaatcttta catttttcaa agttatatgg ataaatactt
    33901 tctaatttaa tcaaagactt tagatctctt gttttatttt cttttataac ttttacaaat
    33961 tcattgtcaa atatatttcc cattttatag tcatccttta tcagtgaagg acaaggatat
    34021 acatctccat cataatttat aaatatttga gagtttcctg cattacatgc acaacatccc
    34081 atttttttat tttttttatc tttacacatt tttaattcat tatctaaaat atacggaatt
    34141 gtaattggaa gaactgtatc ttcaattaaa tagctttctt tattttctag gcctcttcct
    34201 gttggaataa aatatctttc aacaaattct gttccaagct ttttatttaa atctttaaat
    34261 tgtttattta aatgagaatt tttttgtcca aataccatag acagctttat attactgaat
    34321 ccattgtctt ttagcagatt aacagagctt attactttat taaatacacc ttttcctcgt
    34381 gtttcactac aagtattttc atctactcca tcaatactaa tatctatttt ttcaaaatta
    34441 gttataataa aatcaacatt atgttcatct attaaagttc catttgtgga taatattaat
    34501 ttggtatcag gtaatttttg ttttatatat ttaattattt caaaaaaatc ttctcttatc
    34561 aatggttctc caccagttat aattatatga ggagtattta aaactgatat tatatctacg
    34621 atatttttta aatcatatgt actaagtaaa tctttcttat tagaagaaga ttcaacacaa
    34681 caatgtttac aaaataaatt gcatctattt gtcaaacaaa aataaattgc aacaccattg
    34741 cttaacatat caaagtttaa attgttactt ttaaaaattt gatttttgtt cataatatct
    34801 ataagattaa taatatattt tttgtcactt tcatcatcaa aataatttac aaaatcataa
    34861 atttttattt tattttcttg tcctaaaaga aaa
//
LOCUS       DAYAOY010000038        34576 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYAOY010000038 DAYAOY010000000
VERSION     DAYAOY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.038"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            56..790
                     /locus_tag="ACGI6B_001875"
     CDS             56..790
                     /locus_tag="ACGI6B_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9751321.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(933..1424)
                     /locus_tag="ACGI6B_001876"
     CDS             complement(933..1424)
                     /locus_tag="ACGI6B_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFG9751322.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1773..2156
                     /locus_tag="ACGI6B_001877"
     CDS             1773..2156
                     /locus_tag="ACGI6B_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HFG9751323.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
     gene            2664..3047
                     /locus_tag="ACGI6B_001878"
     CDS             2664..3047
                     /locus_tag="ACGI6B_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFG9751324.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3049..3399
                     /locus_tag="ACGI6B_001879"
     CDS             3049..3399
                     /locus_tag="ACGI6B_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751325.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3500..3958)
                     /locus_tag="ACGI6B_001880"
     CDS             complement(3500..3958)
                     /locus_tag="ACGI6B_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751326.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4003..4791)
                     /locus_tag="ACGI6B_001881"
     CDS             complement(4003..4791)
                     /locus_tag="ACGI6B_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9751327.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4930..5367)
                     /locus_tag="ACGI6B_001882"
     CDS             complement(4930..5367)
                     /locus_tag="ACGI6B_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFG9751328.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(5601..5735)
                     /locus_tag="ACGI6B_001883"
     CDS             complement(5601..5735)
                     /locus_tag="ACGI6B_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751329.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            6189..6698
                     /locus_tag="ACGI6B_001884"
     CDS             6189..6698
                     /locus_tag="ACGI6B_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751330.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            6725..6871
                     /locus_tag="ACGI6B_001885"
                     /pseudo
     CDS             6725..6871
                     /locus_tag="ACGI6B_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7104..7640
                     /locus_tag="ACGI6B_001886"
     CDS             7104..7640
                     /locus_tag="ACGI6B_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFG9751331.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(8067..8867)
                     /locus_tag="ACGI6B_001887"
     CDS             complement(8067..8867)
                     /locus_tag="ACGI6B_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751332.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(9062..10660)
                     /locus_tag="ACGI6B_001888"
     CDS             complement(9062..10660)
                     /locus_tag="ACGI6B_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9751333.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            10988..11164
                     /locus_tag="ACGI6B_001889"
     CDS             10988..11164
                     /locus_tag="ACGI6B_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751334.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            11338..12468
                     /locus_tag="ACGI6B_001890"
     CDS             11338..12468
                     /locus_tag="ACGI6B_001890"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFG9751335.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            12709..13059
                     /gene="eutS"
                     /locus_tag="ACGI6B_001891"
     CDS             12709..13059
                     /gene="eutS"
                     /locus_tag="ACGI6B_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFG9751336.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            13067..13516
                     /locus_tag="ACGI6B_001892"
     CDS             13067..13516
                     /locus_tag="ACGI6B_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFG9751337.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            13618..14193
                     /locus_tag="ACGI6B_001893"
     CDS             13618..14193
                     /locus_tag="ACGI6B_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9751338.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            14186..15595
                     /locus_tag="ACGI6B_001894"
     CDS             14186..15595
                     /locus_tag="ACGI6B_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HFG9751339.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            15779..17212
                     /gene="eutA"
                     /locus_tag="ACGI6B_001895"
     CDS             15779..17212
                     /gene="eutA"
                     /locus_tag="ACGI6B_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFG9751340.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            17230..18594
                     /locus_tag="ACGI6B_001896"
     CDS             17230..18594
                     /locus_tag="ACGI6B_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HFG9751341.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            18607..19488
                     /gene="eutC"
                     /locus_tag="ACGI6B_001897"
     CDS             18607..19488
                     /gene="eutC"
                     /locus_tag="ACGI6B_001897"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HFG9751342.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            19508..20161
                     /gene="eutL"
                     /locus_tag="ACGI6B_001898"
     CDS             19508..20161
                     /gene="eutL"
                     /locus_tag="ACGI6B_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFG9751343.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            20172..20873
                     /locus_tag="ACGI6B_001899"
     CDS             20172..20873
                     /locus_tag="ACGI6B_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFG9751344.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            20876..22345
                     /locus_tag="ACGI6B_001900"
     CDS             20876..22345
                     /locus_tag="ACGI6B_001900"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HFG9751345.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            22441..22728
                     /gene="eutM"
                     /locus_tag="ACGI6B_001901"
     CDS             22441..22728
                     /gene="eutM"
                     /locus_tag="ACGI6B_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFG9751346.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            22855..23616
                     /locus_tag="ACGI6B_001902"
     CDS             22855..23616
                     /locus_tag="ACGI6B_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFG9751347.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            23629..24258
                     /gene="eutD"
                     /locus_tag="ACGI6B_001903"
     CDS             23629..24258
                     /gene="eutD"
                     /locus_tag="ACGI6B_001903"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HFG9751348.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            24300..25025
                     /locus_tag="ACGI6B_001904"
     CDS             24300..25025
                     /locus_tag="ACGI6B_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HFG9751349.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            25038..25310
                     /locus_tag="ACGI6B_001905"
     CDS             25038..25310
                     /locus_tag="ACGI6B_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFG9751350.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            25303..25854
                     /locus_tag="ACGI6B_001906"
     CDS             25303..25854
                     /locus_tag="ACGI6B_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFG9751351.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            25866..26957
                     /gene="eutH"
                     /locus_tag="ACGI6B_001907"
     CDS             25866..26957
                     /gene="eutH"
                     /locus_tag="ACGI6B_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFG9751352.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            26959..27432
                     /locus_tag="ACGI6B_001908"
     CDS             26959..27432
                     /locus_tag="ACGI6B_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9751353.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(27552..27647)
                     /locus_tag="ACGI6B_001909"
     CDS             complement(27552..27647)
                     /locus_tag="ACGI6B_001909"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9751354.1"
                     /translation="MGYPNVEHFIRLFNKKYNMSPSKYRKNTSKI"
     gene            27948..28469
                     /locus_tag="ACGI6B_001910"
     CDS             27948..28469
                     /locus_tag="ACGI6B_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9751355.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            28848..30023
                     /locus_tag="ACGI6B_001911"
     CDS             28848..30023
                     /locus_tag="ACGI6B_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9751356.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            30058..31596
                     /locus_tag="ACGI6B_001912"
     CDS             30058..31596
                     /locus_tag="ACGI6B_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9751357.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            31628..32830
                     /locus_tag="ACGI6B_001913"
     CDS             31628..32830
                     /locus_tag="ACGI6B_001913"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9751358.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(32997..33596)
                     /locus_tag="ACGI6B_001914"
     CDS             complement(32997..33596)
                     /locus_tag="ACGI6B_001914"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751359.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            33766..>34576
                     /locus_tag="ACGI6B_001915"
     CDS             33766..>34576
                     /locus_tag="ACGI6B_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751360.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQ"
BASE COUNT        13491 a         4255 c         5749 g        11081 t
ORIGIN      
        1 ttttgtattt aatagagtgt tgctaagtga atatatttaa aatggaggaa actttatgaa
       61 acaaaataaa tatgatgatg atagattttt taataaatac agtaaaatgg aacgttctac
      121 aaatggatta gcaggtgctg gagagtggca tgttttaaaa aaaatgttac ctgatttcaa
      181 aggaaaaaga gttttagatt taggatgtgg atttggatgg cattgtcaat atgcagttga
      241 gaatggagca atttctgctg ttggagttga tatttcagaa aaaatgttaa aagaagctag
      301 aaacaaaacg caacttgata atataaatta catatgtatg cctattgaag atattaattt
      361 tccaaaagat agttttgatg tagttattag ttccttggca tttcattata ttcaatcatt
      421 tgaagatata tgtaagaata taagtaattg tctttcaaat aaaggtaatt ttgtattttc
      481 tgttgaacat cctatattta ctgcatatgg aagtcaagag tggtactgtg ataaagatgg
      541 cactaatcta cactggccag tagatggata ttttgaagaa ggagtacgta agtcaaattt
      601 tctaggagaa gaggttataa aatatcataa aacattaaca acttatttaa atacgttaat
      661 gaaaacagga tttgaaatat tagaaattat tgaacctcaa ccagaagaaa aactgttaga
      721 tacaattcct gatatgaaag atgagctacg tagacctatg atgttacttg tatctgcaaa
      781 aaaaagataa gtaaatataa ataatcatta ttgtagaaaa aaagttgtag aaaataatta
      841 cactaatgga gatgattata ccagtggtat taattacacc agtagtaagg aggcaaaagc
      901 attcgttgct tttgccattt tttaagttgc aattatactt ctacgtagta tatttcgttt
      961 ccattttctt ctataagctt tttcatatct tcaaatttta aaaatacagt agcagtatta
     1021 tcatttggat gtatacctat tttatccatt tttaataaat ctttatcaaa tacaactttt
     1081 acaacagcct ctttatcatt taatatacca aaaggagtca cagctccttt agtaagtttc
     1141 aagtattttt gaagtctttc ttccgatgca aaactaagag gtttactatc aatttgttga
     1201 cgaatatctt ttaaatctgc ttttttgtct ttacctaata caactaaaaa atgttctttt
     1261 cctttagcat cacgaagaaa taggtttttt acaacaaatt ccatatgtaa tgtttttaat
     1321 tcttccattt cttcaattgt ataaactggt ggatgattta cagcctcata aggtatgctt
     1381 aactcatcta attttttata tacttcctgt tgttgattgt tcatgatttc ctcccattag
     1441 aaatttattt tatatataaa taatattatc acagaaaact ttaaaagaaa acgttttata
     1501 taatatagac aatagattat atttttataa agaagcaaga atactcaata aaacttaaag
     1561 aaattaaaca atttctaaaa ataccttgaa aatagaacct tatttgccat ttataaatta
     1621 tataaaaagg gattatataa agtcatatat gaaaaaacta tattatacta tcttttcttt
     1681 ttggaataaa ctatacttta aatttagata tttaatttaa ataaatttta cagtattaat
     1741 agtaaattat cttagaaagg aatgtgcttt atatgtctac agacaaagtt gttggaatca
     1801 tagatgaaga aacggcagaa cttgcaggaa ttgaatatac tggtaaaatt tatgcttctt
     1861 ctggtgttat aaaacatata aagaaaaaac atagatgtca actgagtaag gatattttta
     1921 atgatataat agacactata aaaatggtat taaagtcacc tgaatatata ggaagtcatc
     1981 ctaaaaaacc tggaaaaagt gtggagttta taaaaaaaat tgatgattat attttagtag
     2041 ctacagaatt agatacaaaa aaaggatatt tatatgtatc ttcaatgtat gctataaaag
     2101 aagcaaaatt agaaagtaga gtaaacagtg gaagagtaaa acaatataat ggttaacata
     2161 atattattaa ttttccctaa aatattgaaa aatattatta tataagttat aatattaata
     2221 tacacgtaga tatttattag ttagatttga ggttggaaaa ggcacccaat acgctactat
     2281 agagtagtta atagagatgc aggatacgcc gccctgccaa atctaattaa attaaaaaga
     2341 gaggattaat tcctctctta ttgttttttt gagatactaa tttaatctat aatagaaaga
     2401 ttgaaggtcg tttatacaaa aaaattttca gttatataca ttaaactaat taaataagat
     2461 atttataatc tatatgtgat acatattata tattaataaa agaaaaatat atagaaaatc
     2521 cttataaaaa tttaaattgt aatgaatagg aagtaattaa aaagaatttt tattgatttt
     2581 taaaaattta gttagaaact atatcattat ggtaaaaaat gtattataat gtttagaaat
     2641 attataataa ggagcgatta aacatgaatc tatcgaagaa aatagaagat aaaagaaaag
     2701 ctaaacagag agctgaaaaa gttaagaaag ctaaaattgc tacagctggt gtggtattag
     2761 gagctgttac tggtgcagtg agtggagtac ttttggctcc taaatctgga aaagaaacaa
     2821 gagaggatat aaaagatgct tcacaacaaa ttgctgaaaa gataaatatg aaaactgtag
     2881 atgtaaaagg caaagtatct gagaagctag aggataaaaa aggaaacttt atagaatcaa
     2941 agaaaaaaat aaaaaaatat atagataaca aaaagagtgt tgaaaattct agtgaggaag
     3001 ataaaataat agatatagtt gaaccagtta gtgttactga ggaataatat ggacaataca
     3061 ataacgatta acataagtat aatatatgta ttattagcta tacttgcttg tattgcttta
     3121 gtttatttaa ttattgtact tgttaaacta cataagattt taaataatgt tggaactatg
     3181 ttaaatgaaa atactcaaaa tataaataca actttaaata atcttcctaa tgttgtagaa
     3241 acttttggag aagtaggaga aaatatgaaa gatgtaacag aagtagttac tgaagttaca
     3301 gcagattttc ttgttactaa agacagtgtc aaatcaaata tggatgttat agttgatata
     3361 ttgactataa ttaaaaacat ttttctagat aaaaaataat aagtttaatt aaaataagcc
     3421 tataagatat aatttaaacg accttaaaat agcatttata aggtcgtttt ttattagctt
     3481 gatttgacac tatgtgtttt taataattgg taatattcct ctttatcatt tgaacatata
     3541 tatctatgct tgttgtttca attacatcca taagaacatc tttacattca agctcaaaat
     3601 cttccattcc ttctaaatat tcatcatctt catcaaaaaa gatataagaa ttatatagcc
     3661 attcttcaaa tttttcttca agtatatccc aatacgaact ccaaaataat ccacaacctg
     3721 atatataaga tgctctactt tgatttatat aaacctcctc tagaagcttt tgtaatctac
     3781 tgctataaag aaaatctgct cttccactgc ttgatattaa ttcatttaaa tcttctattg
     3841 acattaattc aactttacca cttttatcat aaggagtata attttcatca acccatttat
     3901 gtggtttgtt ttctctgcat actgttgcta tcaactctac aacttttgtt cttatcattt
     3961 ttttatccta acttatttat tttttataaa ataatcatga ttttataatt tatctatttc
     4021 ttgtaatgct aatttaacag tttcatatct atcagcacca tttattacta catcaccttt
     4081 tacaagttta ttagcttctc cacctacaac ataaaccttt ttacctaaac cttgcttata
     4141 actagacgct tcaattatta aagaatcttt caattgccaa tgtaaaatct gagcagccac
     4201 tttatcagca tcacctgtat aaacgattac attttcatac actttatttt cccctccctc
     4261 ttgagaattt gatatagatt tgtttagtat accttctgct atcaatttag caactttatc
     4321 tttattatta acataataat ctgtatctgc tttactatct acaaaacata cttctattaa
     4381 tattgctggt gcaatagtat gattaagcca ataaagacct ctaacatcag actttgcgcc
     4441 cctatctttg aatactgtag ctaacctagt attcactctt tctgcatagg ttttgccatt
     4501 attagtctta tatattgttt ctgtccctac tggagttgaa gttgtggcat ttgaattaaa
     4561 atgaatctga acagccaaat cagtttcctg tttatttgct atagcacatt ggtctgctaa
     4621 atgattactt gattcatcaa ctcttcctgt ataaacagta gctccaccta ttttaagcca
     4681 tttaacaatt aagtcattca gtattcttgt ttctttgctt tcgtttatat acccaacagc
     4741 tcctgtacct tttccaatta aagtgtgccc tggtattatt actactttca aactaacatc
     4801 tcctttacaa ttaaatttaa cttctattta atatatgcat aacctagaat tttgtttctt
     4861 tttatatctt atacttttat tataacccat tttgtaaaaa tatagaatat taagtaaata
     4921 atgttaaaat tataattttg tatataagac attacttgat tgcaataatt ttatagcata
     4981 ttcatctatt ctatatccag ttctataata tatatgttta attccagctt gaattatagc
     5041 cttagtacaa tttaagcaag gaaagtgagt tacataaata gagcaatttt taactgaaat
     5101 accttctttt gcacaataat aaagggcatt ttgttcagcg tgaatggttc taatacaatg
     5161 tccatctctc atttcatgac caacttcact acaatgctta tctccagaaa tactaccatt
     5221 atatccagta gacactattc tgttttctga attgacaatt atagctccta cataagctct
     5281 atcacaagtt cctctttcag ctactgtttc gcacagtctc ataaaatatt cttgccatga
     5341 gcatctattt tcaagttttt tattcaaatt atatacctcc aaaacttata tctaatatat
     5401 aagatttata ccttaaaaat aatattaaaa acaaatttat acgataaata aaatttatta
     5461 atactgtttt tagtctaaat tatttcattt taaattaaag ttacaaggaa tctaaaatta
     5521 tatatatctt tcttcgtaat attaatgtta caatataaaa aactgtctca ataaaataga
     5581 gacagtttta cagtcaaagt ttataataca agttcatatt tttctttaaa ttcttttaca
     5641 gcttcgggcc atgccttttg ttctgtaata cctttttctt tagctatttt aattgcaatc
     5701 tctcttatca ttgcagcgcc ttctaatatt cccattttta atctcccttc aaatatcttt
     5761 ttgtatagaa tataaatagt gctaagattt attacatgct gcatatacaa tatctataca
     5821 acacataact tgtaaattcc ttttattttc atacgtcttt aaccgacata taattatatt
     5881 caataagttt taaaattatg aactcaatta taattatttt tattttttta gaatctaaac
     5941 actaaaaatt gtaggtaaat ttgtcttatg cctttataaa tatttctaca gttttaatta
     6001 ctggacatat ctatttttat tataaaaagc gattaaaaat cgagaatatc cgcataaaaa
     6061 ctgtatataa gcaaaacggt taaaggaaaa agaagaaatt tacttgaatg ttaaaatgcg
     6121 ttgcttgaag caatgggcag ttttgcctat aatatcggta acaactgaaa gaaaggtggt
     6181 tagccctaat gttaagcgaa aacattaaaa caatcaggaa gtcaaaagga ctttcacaac
     6241 aagaacttgc tataaaactg aatgtagtgc gacaaacaat atctaaatgg gaacaaggac
     6301 tgtcagttcc cgattctgat atgttgattt ccatatcaga ggtgcttgaa acgcccgtaa
     6361 gtactttact tggagaaact attgttgagt cggaagttga taacttaaaa gtgatttctg
     6421 aaaaattaga gattataaac ttgcaacttg cacaaagaaa aaacatgaga agaaaaatgt
     6481 ttcattggtt atttatttta ttatgtgcag tcatattaat agtttttgtg gtcttagggg
     6541 gactaaatag tccttacttg gattggaatt ataccgaccc tgaaaccgct gtttttggag
     6601 tagcttttca tgcttttgag tggctatttg tcagattagt accgattgtc cttataggag
     6661 caattattgg aatctttttg acacgtaaga aaatatgaag taactctgct ttttataata
     6721 cagcaagcgt tccttaaaag cgtcattcta catggcaatc gaggacgact atgtaaggaa
     6781 gaaccttttt gatttccaac taagcgaagt tattgaggac gacagtaaga ccaaaaccgc
     6841 attaagtaaa gtacaggaag caagactgct ggacttatac tctcatgcgt cagatgcagg
     6901 aaatgcgagg tctggtagca tgatttacaa cgatttgtac aacgactgct agtagaaata
     6961 atatggagat aagtattatc tacaaagtca gaaaagaaat gacttgataa tttactcatg
     7021 ttataattat tgatgtgaaa ttaataaatc gaaattcctt ttttggattt aataatactg
     7081 agtaaggaaa aagtgaatta cttttgcaag gaggtgttaa tatcaggcaa gtaaagtctt
     7141 ttgtttttcc agttatatcc ttaatattct tagaccaaat tagcaaagtt cttataggat
     7201 tattcttaat ggactttgaa attgatataa ttgggaaatt tttaagattc aatcctgttc
     7261 aaaatacaaa tctctcttat gggggaaact ttattggtat tctatctaat ttatgggtat
     7321 tggtattgtt taacatttta gttatattag ttattatatc tggatatgct ttttataaat
     7381 caaaaaatga acaaacaagc tattcagtaa aagtaattat gtcttgtgga cttgctggta
     7441 caatatgtag cttgatagat aaattatttt ggggaggaag tttagatttt ttgcagatac
     7501 caagcttttt tattttcgac ttaaaagact gctaccttac tgttgcagaa ataatatttg
     7561 ttgtcatagg aattttgcat aatagagaaa tatcaatgaa agaatacata tatttttgct
     7621 atcgtcagtt taaaaaataa atttagaaga taaatttaag tttcaaggta aacttattta
     7681 atcacagcaa atagttggct tacttaagct tttgggctaa actgtgagca ctaattaagt
     7741 ctctgatatc taacaaacgg agcacaaaaa taaaaattaa atataaagca cagttaattt
     7801 tgattaaaaa gttgactgtc ttttcttttg tagaaattta agcataggta ataaagaaat
     7861 gactgcattt aagcattatg tatataattg gaggcaataa aaagccagca aagcccataa
     7921 aatagagctg tgctagcaac atgatgaatt ataagaagat aatcaaaaac ttacgcttta
     7981 ttttaattta aaaaaatgat tactgtaaag aaatcatttt ttattataat cactgataca
     8041 tcaaatattc taaataaaat ttttatttat ctggaattgt atattttttc tataatatta
     8101 actacagcgt acattgccca agcacagata gccaatacaa atactcccat cattactaaa
     8161 tctaatttaa atacttgaga accatatact atcaagtagc ctattccata tcttgaaact
     8221 aaaaattctc caactattac acctacccat gccataccaa tattaatttt agttaaattt
     8281 attaaatttc ctgtgtttgc aggtaatatg agtttaaata atatttgaga tttagtagct
     8341 ccaaaacttt ttaacatctt aattttttct tcatctatat tcataaaata attataagct
     8401 gaaagtatag taacaacaac agaaatcgtt acagctgtta cgacgattcc ttctattcca
     8461 gcacctaccc aaactataat tataggagct aaagcagtct taggaagagc atttaaaact
     8521 accaaaaatg ggtctaatat ttttgataat ttttctgacc accaaagagc aattgctacc
     8581 aaaataccta atacagtacc tataacaaga ccaagaacag tttcatatac agatattcca
     8641 atatgtttaa ataattgacc actaaatgca tattgaataa ataaattgta aatatcactt
     8701 ggtttgctaa atagaaatgt atttataaca tttaagtcag ctaaaagttc ccagattaca
     8761 ataaaaccaa ttaaaattaa tagctggtag aataatacat ttcttttttc tctttttaca
     8821 cctttaacat agtttatata tccttcagat cttttattaa gcctcatgtg tatctagctc
     8881 cttccataat atgtcgaaat acttttgaaa atcaggaact ttacgtgttt tgagaggcgt
     8941 ttttgttcca tccatgttaa gttcaatatc gtaaatattt tttatggtag ctggtctttt
     9001 agatagtatt gcaaccttat ctgatatgct tattgcttct gctatagcta aaaaagtgca
     9061 actaattgct taaagaaaca gaattatcaa cttcagcacc catataaaca atattaagat
     9121 ttcctaaact gctatcccaa acgatactat taataatagt agataataac aatctcttat
     9181 catctaaact ggcattatca acttcatcat taaatctctt caaattacta acaataatat
     9241 caatattaat actgtcacta ctaatattga tgttactaga ttccaaagta ttaatcttat
     9301 cctttaaaat actaatctca ttatttaggc tctcaatctg ggaaactata taatcagaag
     9361 cagtactacc agtatttttt gcaagctcta taactaaatt tttaataagt ctttccttat
     9421 ctaaaatttg atttttaagt aaattagtct cagatttaaa aatattaaaa acattatcag
     9481 tcttaatgtt attactattg aatgcactta taatagactc ttctttacaa ttcttaattt
     9541 tatctataac taaactttca actaatggac cattaagatt tttacagttg caactatcag
     9601 acccaagtgt ttttttagta ccacaaatat aataatactt taaatccttg tcagtttttt
     9661 tgcctttata ggtaattctc atattagaac cacatttacc acattttata agaccagata
     9721 aaagagcagt ttttccagtt gatatccttg gagttttatc acgattttta tctaattgat
     9781 tttgtacttc aagccatatt tcaggttcta tgactccatg gtgttttgca attgctgcta
     9841 ttgagttaga tgtattttta gcataggtaa ggattccaca ttttctatct ggagtaccta
     9901 caacatccat accactattt ttaaggtaat ctaaaatttc atcattagct tttacatatg
     9961 ctggatttct aagtattcta gataaagaac tagggtcaaa tttgccacca cgaggacccc
    10021 ttattccatt attatagaca tatttatata acttagataa ggacctaagt tctaaatatt
    10081 tattgtatat tagctttaca atctccattt gttcatcatt tacttgaagc ttatacatac
    10141 ttctttcttt tagattttca tctaagaatg tgattttttc actgttgaat ccatgaggtg
    10201 tattgcctcc aagccatcta ccactacggg ctaattcata catattgtct tttatacgtt
    10261 ctgctattgt ttctctttct aattgagcaa atacacttga tataaacatc atagctcttc
    10321 ccattggagt tgaagtatca aattgttctt tgatagatat aaatgatatg cttagttcat
    10381 ttaatttttc tattaaagta gagaagtcag atacatttct cgatatccta tctaatctat
    10441 aacaaactag gtaactaaat ttcttatctt tggcatcctg catcattttc ttaaattgtg
    10501 gtctatctat attgctacca ctaaatccct catcttcata tatcaaaaaa tcttttacac
    10561 caatagactt tgcataatca atacataatt gtatttgatt ttctatagaa tcaccttttc
    10621 cagtaaatct tgattttctt gaataaattg cagctttcaa aattaaaacc ccctataagt
    10681 taggatatat atttgaaact atttatctac aaccttttaa gtaaacttat tagaatgaat
    10741 ttattaatta ggtaaattta tataattcta ctttttataa tatatgacta tttattttat
    10801 ttctttacta ttcaagttct aaatcaataa taaattttga tatttaattg tatcaggaca
    10861 aatctctaat ttcatagatt attaatataa aatcataggt tagtgtgaaa aagttgaaat
    10921 ttaaatattt taaaagataa tttttttgaa ctaataggta taacaaaaat ttataggggg
    10981 attgcatatg gaaatcaatg ttatagaaat tttccctaaa gataaagcta aacttaataa
    11041 aatagaaatg gataaagcta gttggtttgt aaatataata agtaaaaaat atcctaaaga
    11101 agctttaaat gaagcattta gtactttaga aaaagaatta aatataagta aagctaatac
    11161 ataagctttg attattgatt ttatatacta ttggttttat acgctatgac ttgacattat
    11221 attaatcaaa tagtatattt ttaaatatag cacaatgaag tgttaaaaga gcaatggagc
    11281 tccatagata aagtctatgg agcttttttg cgtatttaac ttaaggagga taggactttg
    11341 aataactttg agattaaaac tagaataaaa tttggagaag gctcactaaa agctttgcaa
    11401 gaattaaaaa acaaaaatgt actaataata acagaccctt ttatggtgaa atcaaaaaca
    11461 atagataaaa taacatctaa tttaataaac tcaacatatc aagtttttga agaaatagta
    11521 ccagacccac caatagaagt agtagtagca ggaatagaag catttaaatc tataaatcca
    11581 gacgtaataa tagcattagg tggtggttct gcaatagatg cagcaaaatc tataatggac
    11641 ttttctaaaa aaatattaaa aattaacgaa gtagagttta tagcaatacc aacaacaagt
    11701 ggaacaggtt cagaagtaac atcatttgct gtaataacag atagtcaaaa aaaatgtaag
    11761 tatccattag tgagtgatga tttacttcca gatatggcaa tactagaccc agaacttgta
    11821 aaaactgtac caaattttat aacagcagat acagggatgg atgtattgac acatgctata
    11881 gaagcttacg tatctacaaa ttctaatgac ttttcagatg cactagctga aaaagctata
    11941 aagttagtat ttaaatactt aataaaagca tataaagatg gaaatgacat agaagctaga
    12001 gaaaaaatgc acaatgcatc ttgtttagca ggtatggcat ttaaccaagc atcacttggc
    12061 ataaatcatt ctattgcaca tgtattaggt ggaaaattcc atataccaca tggaagaaca
    12121 aactctatat tacttcctca tgtaatagaa tataattcat caatagtagg atatactaac
    12181 acaacttact ctgctacagc taaaaaatat gcacaaatag cagaattagt aggacttggg
    12241 agtgctaata taagaatatc tgttagaaat ttagtaaatg aaataaagaa actacaaaaa
    12301 gaaatgaata tgccaactaa attgacacaa tgcaaattaa gtttagaaga tataactaat
    12361 gaagaaagag aaatagcaaa acttgctata aaagatactt gtacattaac taatcctaag
    12421 gtaccaactg aagatgatat aataaacata ttaaagaaca ttaaataact acattttatt
    12481 acatgtaaat ataatattat acaaataggg aaaatgtttt taaaaatata aattatatat
    12541 tgacaaatat aaaacataaa actataatga aagtatcaca aaggcgtgat actttagtag
    12601 aaagcaatgg agcttcttag gaatattaat attattctta tggagctcct tttattttaa
    12661 tcaaatagct ttttaataaa ctatattcaa agaaagcagg gaataaattt gactgaagaa
    12721 tctaagcaaa gagttattca agaatatgtt cctggaaaac aggttacatt agcacatatc
    12781 atagctaacc ctaatgaaga tatatataag aagttaggat tagtacttga taaaaaagat
    12841 gcaataggaa tacttacaat aacaccaagt gaagcgtcaa taatagcagc agatgttgct
    12901 acaaaggctt caaatgtatc tttaggtttt atagatagat ttagtggttc agtagtaata
    12961 tcaggtgatg taagttctgt agaatcagcg ttaaatgatg ttttagaagt cttaggaaat
    13021 atgttaaact tctcatctac aaaaatcaca aggacataat aatacaatga agaatataat
    13081 atttatggga aagacaggta gtggtaaaac taccttatgt caaaaactag accagttaga
    13141 attaaagtat aaaaaaactc aatcagtgga actttataat aatgcaatag acactcctgg
    13201 tgagtatatg gaaaatagaa acctatataa tgccttaata acaacatcag cagatgctaa
    13261 aattatagca atagtgtatg atgccacaca agaagaaaat tatatagcac caggttttgc
    13321 cagtatgttt tgcaaagacg taataggaat tataactaaa ataaataaag ctactgaaaa
    13381 cgaagtcaat atagcttatg aaagactatc actagcagga gcaagtcaaa tctttaaaat
    13441 agacacagtt gataatattg gacttgaaaa attatttaaa tatctagaaa aatataaaaa
    13501 tatagatgaa gtttaagggg aaatattttt ttattcaaaa atattattaa agtatgataa
    13561 aataaaaaaa tgtataaact attttaagaa gatttaatct ttaagcgagg taggaacatg
    13621 gggaaaaaaa tactattggt tgatgatgaa ccactgttaa ggatggatac aaaagatata
    13681 ttagaaagtc atggctatga aataataggc gaagcaagtg atggatttga agctgttgaa
    13741 atgtgtaaaa aacacaaacc tgatttagtt ataatggata tagagatgcc aatacttgat
    13801 ggtataaagg caggcaaaat aatatgtaaa gaaaatatag ctggtggagt acttttatta
    13861 acttcatttg aagataaaga atacgtagaa aaggctaaaa gtataggtgc ttatggatat
    13921 ttagtaaaaa cagcaagtga aaaatcactt atcccaacca tagaaatgtg tttaaataag
    13981 gtaaatgaat ttgaaaaaat gaaaaaagat ttagaaaaag taaattctaa attgaatgaa
    14041 agaaaattag tagaaagagc aaaaggtata cttataagag agtttaagat ttctgaggat
    14101 gaagcataca ctagaataag aaaattaagt atggataaaa gaactacaat gatggaaatt
    14161 gccaaaatga ttataatagg atacgatgac taatacgata aaaaaaatat caactgcaca
    14221 aacatactta aataataacg aaattgaaaa aattatagaa gtatcaaaat ccttagattt
    14281 aatggcttct ttttatgaag ctgatgtttt tatagatgta ctaagtaaat ttgatgatga
    14341 agcaattgtt gtggcacatg gtagacctaa agaaaagtca atgtactgtg aaaatgttat
    14401 aggtaaaaag gctttaattg aaaatgaacc aggagttata aagtgcttaa aaacaggaga
    14461 aagagtaaga gatattaaag ctttaacaca ggaatataaa ctggtcaaac aaaatattca
    14521 gccaataaca ctagatgaca aagttttagc agtactaata gttgaaaaag atataagtag
    14581 tgaagttaag aatgagttta atgctagtga agacgaagaa aataaaaatt tacatcagtt
    14641 tataaaactt attggaaata atgacttttt agttgataat ttaaattcag ccatattaat
    14701 atttgataaa aatggaaaat taaaaattaa aaatcgtatt gcaactgaga tatacaaaaa
    14761 tcttgggttt ggatatgata tacaagatat gcattacaat gatttagata tagaaaccaa
    14821 cacctttgaa aatatattaa tggatgaaaa ttatgatgag acaaatgaag tttcaatagg
    14881 taatatgttt tttaaaataa agaccatata catcaaagat gatgaattta aggtttgcaa
    14941 aatagtacaa gatataagtg atttaaagag aaaagaagaa gaattagttt tgaaaacagt
    15001 ggctataaga gaagctcatc atagagtaaa aaataatctt catacagtaa tatctataat
    15061 aaaaaaacaa agtagattat ctcaaaatga tgaagtaaag cagtgtttag ataacataat
    15121 aaatagggta tttgcaatac tttcgactca ttatttacta tctaaagaag ttgataataa
    15181 tatatctata cgagaaggta tcaatttatt agtgtcaaat attcaaggtg gctatttatt
    15241 tagtaaggat atagatatac gtataactgg tgaagatttt aaaattagtg gagaaaaagc
    15301 aacagcaata cttttggtta taaatgaaat actacaaaat tgttatgacc atgcattttc
    15361 ggatagagaa catggtacta tacaaatatt aataaataaa gataacgact atagaaatat
    15421 tgctattata gatgatggaa ttggatttga tagtaataat ataaataaaa atagcttagg
    15481 gacatatata atagatagtt atataacaca gatgttaaaa ggaaatatag agagaaaaag
    15541 tagtgagaat ggtacagaag tattaattac tatacctaca caaattaata tttaaataat
    15601 aactcatcta caaagtagta gttttaattg agcaaagagg cttaaggtta ttaatagatt
    15661 aagttaattg aatgtattta taaaaattat aagtatatat tcaatttaac aggtatttat
    15721 tataacttta agcctctttg cttgtttaag acatacttaa attttaggag gtgagaaaat
    15781 gagagaagag ttattaagtg ttggtattga cattggaaca agtactactc agttaatttt
    15841 ttctaaatta atagtagaaa atactgaatc aaatttttcg gtacctagaa taagtatagt
    15901 agacaaagaa attatttata aaagcaaagt ttatttcacc cctcttattt caaataatga
    15961 aataaatggt caacaaataa aagatatagt tgaaaatgaa tataaaaaag caaatatatc
    16021 taaagaagat atacatacag gagcagtaat aataactggt gaaacagcta gaaaagaaaa
    16081 tgcaaatgat gtacttcata ctttgagtgg atttgctggg gacttcgttg ttgcaacagc
    16141 aggacctgat ttagaaagta taatatcagg aaaaggagct ggagctcata tttattcaaa
    16201 agaacattca actacaattg taaatataga cataggtggg ggaactagta acatagcctc
    16261 atttaaaagg ggagaagtaa ccgatactgg gtgtttggat ataggtggaa ggcttgtaaa
    16321 gatagataaa ataagtaaaa aaataactta tatatcacct aaaatagata aaatcataaa
    16381 agataataat ttaaatcttc aagtaggcac aatggctact gtagaaaata caaagccaat
    16441 agtggatata atggttgact tattaatgga aagcatagga ttaaagaaac agacaccttt
    16501 gtttgatttt ataataacca ataaatcaat aagtatagat gaaaatatta aaaatgtttc
    16561 attctctggt ggagtagctg attatattta ctatgatgga gaaataaaag attattttga
    16621 atatggtgac ttgggaattt tacttgggca agcaataaaa aattgtgaat accttaaaaa
    16681 agtaaatgta gttaaatcta tggaaacaat aagagccaca gtggttggag caggttctca
    16741 tactactgaa gtaagtggaa gtacaataac ttacactagc gaaaagtttc cacttaagaa
    16801 cttgccagtt ttaaaattat caattgatga agaaacaggc acttcaaaac aaatcagtga
    16861 agctataaaa aataaactta attggtttaa actagaaaat gatttacaga aagttgctat
    16921 agccatgata ggaaagaaaa atccaacctt tttagatata caagagtatg ctaaagggtt
    16981 tgtagatgga atgcaacaaa tcatagaaaa tgatttacct tttatagtaa ttgttgaaaa
    17041 tgatatggca aaagtgttag gtcaagcaat atatgcatta ttaaattatg aaaaagaaat
    17101 tgtttgtata gacagtgtca aggttgaaaa tggagactat atcgacattg gaaaacctat
    17161 agttgaaggt aaagttttac ctgtggtaat aaaaacatta gtttttaatt aatagaagga
    17221 ggtaagtaaa tgattttaaa tacaaaatta tttggacgta cttatcagtt taaaggctta
    17281 tatgatgtaa tggctaaagc taatgaagaa aaatcaggag ataaattagc aggtcttgca
    17341 gcagaatcag ctgaagaaag aatagcagca aaagttgttt tatcacacat aaaattggag
    17401 gatataagaa ataatcctgc agtggcatat gaagaagatg aagtaacaag aataatacaa
    17461 gatgctgtaa atgaaaaagt ttatgaagaa ataaaaaatt ggacagtctc tgaacttaga
    17521 gaatggctat tggattcaaa tactacaagt cttgatataa gaagaatatc aagaggatta
    17581 acttctgaaa tgatagcagc agttgcaaaa ttaatgtcaa atatggactt agtttatgca
    17641 gctaaaaaga taaaggttac tgctcactgt aatacaacta taggagaaca aggaacatta
    17701 tcagcaagac ttcaacctaa tcatccaaca gacgacccag atggaatact tgcatcactt
    17761 cttgaaggtt taacttttgg tataggagat gcagtacttg gattaaatcc agttgatgac
    17821 tctgttgaga gtgttactaa agtattaaaa agatttgaag aagtaaaaca aaaatatcaa
    17881 ataccaactc aaacatgtgt attggcacac gttactacac aaatggaagc aataagaaat
    17941 ggtgctcctg cagatttaat attccaatct atagcaggtt ctgaaaaagg aaatgaagca
    18001 tttggattta atggcgacac tatagaagaa gctagacagt tagccttaaa acacggtaca
    18061 gctacaggac ctaacgttat gtattttgaa actggacaag gttcagaatt atcttcagaa
    18121 gcacatcatg gaatagacca agttactatg gaagctagat gctatggatt tgctaaaaag
    18181 tatgaaccat tcttagtaaa tacagttgtt gggtttatag gacctgagta cttatatgat
    18241 gctagagaag ttgtaagagc aggacttgaa gaccacttta tgggtaaact acatggctta
    18301 ccaatgggtg ttgatgtttg ttacactaac catatgaagg ctgaccaaaa tgatatggaa
    18361 aacttagcca tactattaac aacagcagga tgtacgtatt ttatgggaat acctcatgga
    18421 gatgatgtta tgcttaacta tcaaacaaca ggataccatg aaacagcatc acttagagaa
    18481 atgtttggac taacagcaat aaaagaattt gatgcttgga tggaaaagat gggattctct
    18541 aaagatggta agttaacaga gttggcggga gacgcatctg tattattagg ataggaggta
    18601 tttaatatga atgagaagga tttaaaagca ttggtagaac aattagttgg acaaatggtt
    18661 ggagaattag atactaatgt tgtgtctgag actgttaaaa aggcaactga agttgtagta
    18721 gacaacaatg cttgtataga tgatataaca gaagttgata taagaaagca attactagta
    18781 aaaaatccta aggatgcaga agcttattta gatatgaaag caaaaacacc tgcaagatta
    18841 ggtataggaa gagctggtac tagatataaa acagaaacag ttttaagatt tagagcagac
    18901 catgctgctg cacaagatgc tgtattctct tatgtagatg aagaatttat aaaagaaaat
    18961 aatatgtttg cagtggaaac tttatgtaaa gataaagatg aatacttaac aagaccagat
    19021 ttgggaagaa aattttctcc agaaactata aataatataa aatctaaatt tggtacaaat
    19081 caaaaggttt taatattagt tggagatggg cttagctcag ctgcaataga ggcaaattta
    19141 aaagactgtg tgccagctat aaaacagggg ctaaaaatgt atggtataga ttcaagtgaa
    19201 attttatttg ttaaacattg tagagttggt gctatggacc acttaggtga agaattaggt
    19261 tgtgaagtta tatgtatgtt ggttggagaa agaccaggat tagttactgc tgaatcaatg
    19321 tcagcatata tagcttataa gccatatata ggaatggcag aagcaaaaag aacagttata
    19381 tcaaatattc acaaaggtgg aacaactgca gttgaagcag gtgctcatat agctgaactt
    19441 ataaaaacta tgttagataa aaaagcttca ggaatagacc ttaaataaag tcgagggagt
    19501 gtagaatatg aaaaatgatg taattcgtcc taatatacta ggaattaaaa taatttcaaa
    19561 tataagtcca gaaatggcac aaaaactaga attaaaatca catcacaaaa gcttaggttt
    19621 aataactgca gactgtgatg atgttacgta tacagctctt gacgaagcaa ctaaagcttc
    19681 agaagttgat gttgtatatg caaaatcaat gtatgcaggt gctgcaaatg cttcaacaaa
    19741 acttgctggt gaagtattag gaataatagc aggtccaagc ccagcagaag taagaagtgg
    19801 tctaaatgca gtagttgatt ttctagagta tggagcaact tttataagtg caaatgatga
    19861 tgattcaata gcatactatg ctcactgcgt atcaagaaca ggtacatacc tttcagaggt
    19921 agcaggaata agagaaggtg aagcattagc ttatttagta gcaccaccac ttgaagcaat
    19981 gtatgcacta gatgcagcaa tgaaagctgc agatgttaag atgtgtgaat tatttgcacc
    20041 tccaactgaa acaaactttg gtggagcatt acttacaggt tctcaatcgg cttgtaaagc
    20101 agcatgtgat gcatttgcag aagcagtaaa atcagttgca gacaatccaa caggatttta
    20161 aggatagtta gatggatgct ataggcttaa tagaagtaat aggatatgtt gctgcaatag
    20221 aagcttctga tgcttgttta aaatctgcaa atgttaatat tgtaagagtt gataaggttg
    20281 gagcaggtat tgttacttta acaatatgtg gagatgtcgg tgctgtaaaa gctgctttag
    20341 aagcaggtga aatagcagct tccagagttg gtacattaag aacatcacat attataccga
    20401 gaatccataa tgaagtaaca aatgctttat tcaaaactaa agaacctaaa gtctgtgaag
    20461 tttcagaaga taaagaaaaa aactttgaga ttacattgga aatagctgat aatgtatcga
    20521 aaaatttatc agaaaaagaa aatgtaattt tagatgtcaa tgaaaacagt ggagatactg
    20581 aaaatttata tagtgatata gataaaacaa acaaaaaaat tgaaacagtt gatgatagtt
    20641 taacagaagc taatgaggaa aaaatagaaa acactaatga taaagaagta ttggaacaag
    20701 ttaaaataga aaattccgat tttgatagag atgacctatc aaagaacagt gtaaaaaaac
    20761 ttaaagctat ggctaagaaa ttagattctt caataactta taaagactta aatgttctta
    20821 aaaaagaaga attaataaac ttagttaaaa agcttagtag aagggataga tagttatgac
    20881 acttttagat aaagatttag tatctataca agaagtaaga actcttgtaa gaagatctaa
    20941 agtggctcaa gagaaacttt ctaaaatgag tcaagagcaa atagataaaa tagtaaaagc
    21001 tgttgtagat gcagcattta ataatgctac taaattagct aaaatggcaa atgaagaaac
    21061 tggatttgga aaatgggaag ataaagtaat taaaaatact tttgcatcta tgggagttta
    21121 tgaaacttta aagaacacaa aaactattgg aataattggt gaagataaag aagccaatac
    21181 gatagatata gctacaccag ttggagttgt tgcaggtctt ataccttcaa caaacccaac
    21241 atcaacagta atatacaaaa ctataatatc tctaaaagca ggaaatacta tagttttctc
    21301 tccacatcca aatgcaaaaa actgcatact tgaaacagtt aatataataa gtgatgctgc
    21361 tataaaggct ggagctccag aggattcaat attatgtata actaatccaa gtatagttgg
    21421 aaccaatgaa ttgatgaaac ataaagatac aaacttaata ctagcaactg gaggagaagc
    21481 aatggttaga gctgcttact cttcaggaac accagctatt ggagttggac caggaaatgg
    21541 accagcattt atagataaat cagcaaatgt taaattagca atcaaaagaa taatagattc
    21601 taaaactttt gataatggtg ttatatgtgc atcagaacaa tctatagtaa tagaaaaatc
    21661 tatggaagaa actgttttaa aagagcttaa aaatcaaggt gcatatgttt taaatgagga
    21721 ggaaagtgaa agattagcaa aattcatatt aagagctaat ggtactatga atccacaaat
    21781 agttggtaaa agtgttgagg taatagcaaa tttagcagac ttaacaaatg tacctaaaaa
    21841 tacaaaagtt ctagttgcaa gagaaaataa agttggtcac aatgtagcct attcaagaga
    21901 aaaacttaca ccaatactag cgttctatgt tgaagaaaat gtagaagcta ttatgaacag
    21961 atgcagagaa atattgttaa atgaaggtgc aggtcataca ttctcaatgc atgtaaatga
    22021 tgaagaattg gtaaaaagat ttgcccttaa tatgccagta tcaagaatac ttgtgaatac
    22081 tccttcagca cttggaggaa taggtgcatc aacaaacctt gtaccagcac taacacttgg
    22141 ttgtggtgca ataggtggaa gttcaacttc aaacaatata ggaccacttg atttaataaa
    22201 tgttaaaaga gtagcttatg gaatcaaaga aatagaagaa cttggaagag ataaatctca
    22261 agttgaaaaa tcaatgtttg actcaatgga taaagaagaa ttaataaatc ttcttgttca
    22321 aaaactagtc aaagaactag cataactaaa ataaacaatg aaacttatgg ctatcgctat
    22381 aaatttctaa aaatataaac tataaaaaac tataaaatta aatttttgga ggataagatt
    22441 atggcaagtg caaacgcatt aggaatgata gaaactaaag gattagtagg agcaatagaa
    22501 gcagcagatg caatggttaa agctgcaaat gtacaattag taggaaaaga gcaagtaggt
    22561 ggaggattag ttactgtaat ggtaagagga gatgttggag ctgttaaagc tgctacagat
    22621 gcaggagctg ccgctgccga gagagttgga gaactaatat cagttcatgt tataccaaga
    22681 ccacactttg aagtagatgc tatattacca aaggtatcag ctgagtaata taagaataat
    22741 tataaaattt aaattttgat tgaaaaagag gctatctcaa aaatgagtct tactaataaa
    22801 ctcaatgtat tttgaggtag tcttttttat acatctaata ggagatgtga taaaatgggt
    22861 gttttaacag aaaacgaagt aaaaagaaaa attggaaaaa acaaaaataa tataactgaa
    22921 ttttatatag aaaaaggtca aataataact ccttctgcaa aatcttattt agctgagaga
    22981 agtattgagc ttaaatatgt tgataaactt tcagatgtaa acaatgaacc taaagaagtt
    23041 gtaaaagaag tagttaaaga gaaaaaatat agatatacta ctgtatttgg cgctaaattg
    23101 gatgaaaaac cagaacatat gactcaatta aatggaaatc ttttagtttt taaagaccat
    23161 aaaagaataa tattgagagg aaaaatagat tctcttgaat cagaaatact aaaggtacag
    23221 atactttgtc agaaactaga tatgccaaag cttgttaagg aattgcaaga agtattagac
    23281 tttgtaagaa atataatgag atgtgaagtt ttagatgaaa agctagggga ctttaaactt
    23341 ttaggattaa ctcctcaaga acttagagaa caatctcact atcctaaaaa atactttggt
    23401 ataggacatg agttcccaag ttatgaaatg ggagaagtag tagtagctat aaatagtata
    23461 agaagtaata cacgagaggt tgaacttgca gcctatgaag catttaaggg tgagtatgga
    23521 aatgttcaca gagaagattt aataaaggca cttaatagat tatcgtcagt attttggata
    23581 atgatatata aggttagaac taataagtat aagtagttgg aggacataat ggagaatcta
    23641 ttaaatgaaa tagtggaaga agtaataact agagttaaaa aagaagcatt tatagaagtt
    23701 gaagcatcag gaagacacgt tcacttaagt agagaagatg tcgataaatt atttggagaa
    23761 ggatatactc ttacaaaact aaaagaccta tctcaaccag gtcaatatgc atgtaaggaa
    23821 agagttacaa taactggtcc taaaggaagt ataaaaaatg tagttgtact tggaccatgt
    23881 agaaatgaaa ctcaggtaga aatatcttta acagatggtt caactcttgg actaaaagct
    23941 cctataaaac aaagtggaga cttagaaggt actttaagta ttaaaatatc aacacaatat
    24001 ggtgaagttg aacttgataa ggggttaatg gttgctaaaa gacatataca catgactcct
    24061 aaagatgctg aaaaattcaa tgtatgtgat aaagagatag ttgaagctaa ggtgatgggt
    24121 caaagacctt taacatttga tgatgtagta ataagagtga gtgacagttt taaaacttat
    24181 atgcatatag actatgatga agccaatgca tgtggttatt caaaaggaac tgttgctaaa
    24241 ataataaaga aagcgtaaat ttaacctact atataaatta cctctaggga ggatttaata
    24301 tgaattatga taaccttgta gatttaataa cggaagaaat ctataaaaaa ataaatagta
    24361 atgaaataaa aatatctaat aaacctaaag ctgtgattgt atttgaacaa gataacaata
    24421 aatttaactt attaaaagat gaatttgaaa tagtagaatt tgataaaagt ataagggaat
    24481 gtgaaatagt gatagtttca agactttgta tgagggggct atcgaatata gcacttggaa
    24541 attcaacaag tgatgaagaa agatttatac taaaaatgat tatgaagggc aaagaagtat
    24601 atgtcctaga tgaaggaatt gaatacaaaa aatataagga tactgcacca aaagcattat
    24661 ataagaaatt tatgtcattt gaagatgaaa tatgtaaatt tggtgtagaa attataaaag
    24721 acttaaatgt tataactaaa aataagttaa atataaagaa agaactagaa agtactagag
    24781 gttctaaaaa agaagataca aaagttttag ataaagatgt tttaaattta accagtgtag
    24841 aaagtgtaaa aatagataat gaatttagtt tagatttaag gaataaaaaa cttatatcgg
    24901 aagctgactt aagaaaacca actataaatg gagttaaaaa catattagtt aataaaaaaa
    24961 gtataataac accattagct gtagatttta tgagaataca ccatctcaaa ctaaagaaag
    25021 tgtaaggagg taattttatg gatataggaa aagttgtagg taatgtttgg gctacaagaa
    25081 aagatgaaaa attatgtggc caaaaacttt tagttgtaaa aattttaaaa actaaagaca
    25141 catacaagga aggcttcatg gttgcagccg ataatgcagg agcaggaaat ggagacttag
    25201 ttcttatatc caaaggttcc tcagctagag agtcgattga aaaatctcat gttccggtag
    25261 atgcaactat agttggagta gtcgattcgt ttgaggtgtt tgatgaataa aagtatagga
    25321 gctatagagt ttaaaagcat tgctaaaggt atagaagtat ccaatgaaat gataaagaaa
    25381 tcttcagttg atgtattgta cttaaaaagt atttgcccag gtaaatttct aataatagta
    25441 ggtggagaaa cttcttatat aaatgaatgt gtagattatg gaataaaact tggagaagga
    25501 tatattgtag ataacttcgt aataaatgca atttcacaag aaatattaga tggatttaaa
    25561 aataagtatc aaaagctaga tagtatagtc tctataggcg ttgttgaaag tagtaaagta
    25621 tgcgctggaa taaaaatgct tgataagact ttaaaatctg gcgacttagt attagtaaaa
    25681 cttcaattat cttttgctat aggtggcaaa ttagtatata tagtagcagg agatttaagt
    25741 agtttagaat atgcattaaa agaaagtgaa aatgtagtaa gagaaaaaga agtaatatat
    25801 aagactgtaa taccatcagt agacagtcaa ataataaaaa gtctaataaa ataatggggg
    25861 gaattatgag tataaacgaa attataatct atgtaatggt agtcttcatg gtacttggag
    25921 ctatagataa atgtataggt aacaaatttg gtcttggaga acaatttgaa gaaggtataa
    25981 tggcaatggg gtcacttgca gttgcaatgg ttggtgttat atgtttagca ccagttcttg
    26041 ctgatgtatt aagaccaatt gtagtaccag tatttaatat acttggagct gacccagcta
    26101 tgtttgctgg aagtttatta gctaatgata tgggtggtgc accacttgca cttgaacttg
    26161 cgaaagaccc taatgctggg ttatttggtg gattaatagt aggggctatg atgggaccta
    26221 caatagtatt tataatacca gttgcgcttg gaataattga aaaagaagac caaaaattct
    26281 tagcaacagg aatacttgca ggtataataa caatacctat tggagcattc gttggtggat
    26341 tagttgcagg attcgaggta atgatggttc ttagaaactt agtgccaata ataatatttg
    26401 cagtagttat agcacttgga ttattaaaat tcgaaaatgc tatgataaaa ggattcacat
    26461 actttggaaa aggtgtagta atagttataa ctttaggact tgctgctgcc atagttgaag
    26521 ctttaacagg aatagttgta atacctggaa tgaaaccaat cagtgaaggt atagaaatag
    26581 ttggagatat agcaatagtt ttagcaggag cattcccatt agtgtttgtt ataacaaagg
    26641 tatttaataa accattaatg gctttaggaa aagttcttgg aatgaatgat gtatcagcag
    26701 caggccttgt tgcaagttta gctaactgta taccaatgtt tggtatgatg aaagatatgg
    26761 acaatagagg aaaaataata aatgtagcat tctctgtatc agcagcattc gtatttggag
    26821 accacttagg atttacggca ggatttaatt ctaatatgat aacaccaatg atagttgcta
    26881 aattagttgg tggtataaca gcagtattgt tagctatggt tatcgcaaat aagacactaa
    26941 agaaggagaa tgtataagat ggatatatca aatatagata aaaatttaat tgaaacatta
    27001 gtaagacaaa taatagaaga aaaaataagt ggaactaaag acacagtaga ttttgttaga
    27061 aataaagaca ttagtggaat aacttctata aagttaccaa ctgtaaaagt tagtgaatct
    27121 gatagattag atacagggaa tccaagtgat gtagtttaca caaaagattt atttacatta
    27181 gaggaaagcc caagattagg ttgtggtatg atggaaatga aagaaacaac ttttgattgg
    27241 actctaaact atgatgaaat agattatgta atagatggaa ctttagatat aataatagat
    27301 ggaagaaagg tttctgcttc ttcaggtgaa ttaatattta taccaaaagg aagtaagata
    27361 caattttctg tacctgatta tgctagattt atatatgtaa catatccggc agattgggca
    27421 tctcaaaatt aataaaaatc aagttaagtt aataataatc aaataaataa tttaaaatac
    27481 aaactcttaa ataagcacac atatttaaaa aagctatgaa ttaaaattca tagctttttt
    27541 tataaagaat gttatatttt agacgtattt tttctatatt tactgggaga catattatat
    27601 tttttattaa ataatctaat aaaatgctct acatttggat aaccaatact attttactga
    27661 taatcttttc cttatcacta atcttacatt ttaaaatatt atttttactc gtaatattat
    27721 cataagaatt tgtttcttat ttaaaactaa ttagaattat aaagtttatt gccatgttat
    27781 taataaaata aatccagtta aatcgagtgc tagttaaagt aataagaaac ttttacaatg
    27841 gtttccttta tcgattgaaa taaaagttaa tatggtataa tatctaaaga gagaatctta
    27901 tcaattagaa tatgcataga aacaagtggc ttaaaaggag acgttatatg gaagagatag
    27961 taaagaagga acaagcaagt atgaatgata tttttttgta tcttaaagct aaatcaaact
    28021 cattatataa atttgtaata ttatataatg agtttatcaa taaacctaaa gattatggaa
    28081 ctggggaact tataaacatg gttgaagtac atatgctaac agcgattgaa gaaaatccag
    28141 gaataagtcc aactcagtta gctaaaatat ggaacagaac taaaggtgca atatctcaaa
    28201 ctatatctaa attggagaaa aagggttata taactaaaga aaagaaagaa ggaaatgcaa
    28261 aaactatatt actatatcca accacatcag gagtagagtt aagtaaggca cataaactat
    28321 atgacaccat agatgttaca agaacatttg aatacttaat caaggagtgt acattagagg
    28381 agatagatag tttttataaa gtaattgatt tgtttattaa tttattagaa gaatctccgc
    28441 aggaaaataa tgattctgaa aaagtgtagc tgagattgaa gttgaataaa gagaaataga
    28501 tattatagaa gtagtactta agtacacatt atataaaaat ataataggta taggattaaa
    28561 aagtttagtg gtttaaagtt taaccactaa acttttttaa tgtctataat ctactattat
    28621 ttaatttact aaaaataaaa aaactcatta tattaagata aaaatagaaa aataactaga
    28681 aaaatactat aatatatcag cttaatacag aaaaaagtta aaaaatttga gaaaaataaa
    28741 aatgaaaacg ttttatattg acatagagaa aaagtagtga taccatttaa atatagttta
    28801 gtagctaaac tatatttaaa ttataataac tattttaggg ggatgttatg aatttaaaac
    28861 aattaagtga aaaatacgaa gaatatgtaa ttcaaatgag aagagaattt cataagtatc
    28921 cagaattatc ttttaaagaa ataagaacaa cccaaagaat aagggaagaa ttacaaaaaa
    28981 taggggtccc ttttgtatct attgaaccaa atatcgtaat agctactata caaggcaaaa
    29041 atgaaggtaa gacaattgca attagaggag atatagatgc gcttccaatg caagaagatt
    29101 cagatgttga atacaaatca gaatatgatg gaataatgca ttcttgtgga catgatgctc
    29161 atggagctat gttactagga cttgcacata tgctaaatga tataagaaaa gatttaaaag
    29221 gtagagtatt attagttttt caagcagctg aagaagttgg tggtggacac aaggagataa
    29281 tacagtactt taaggaaaat ggaggaccag atagattaat agctactcat gtttggtccg
    29341 atattgaagc tggtaaaatt tctgttgaac caggacctcg tatggctggt gggtctggtt
    29401 ggagaataga cataacaggt agaggaggtc atggttctag accagatcaa agcatagatc
    29461 ctataaagcc agcttgtgat atagttttaa aagtatcttc tataccagtt aatcatataa
    29521 gtgtactaga acaatgtgtt gtacatgcat gtgcaataca tggaggaact acaataggaa
    29581 atgttattcc aaacaatgca gaggtaatag gtggatatag atattttact gaagaatcag
    29641 gcaaaaaagt gtttgacatt ataaagcaaa tgagtaatgg tgttggacaa gtttatggag
    29701 tggatgtgaa ggttactcat actggaggca taggacctgt tatcaatgat gaagaagcag
    29761 ttaagatggc taaagaaata gtttctaata tagatggatt ggagttagat tcttatgagc
    29821 caatatgtgc atcagattgt tatggtgaaa tattaaaaga gtattctggc ttttattgtt
    29881 atctaggggt aggtaacaaa gaaaaaggaa taatatatgc acaacaccat cctaagtaca
    29941 atatagatga agatacatta aagttaggtt gcgaatttat ggctaaatat gcagttgaat
    30001 ttttaaataa aaataataac taaaataaat cgaattttat aaatgggggg gatgaaaatg
    30061 caaggtgcat taaaggaaaa taagaaaaaa agtggtggag gatttttagg atggattgaa
    30121 aaagttggga ataaaatgcc acatcctcta gcattattta catatattac attgatagta
    30181 gtagcattat ctgcaatagc aagcttttta ggtttttcag caacaagtcc tgctacagga
    30241 aagttaataa aagttatgaa cttaataagt gcaaaaggtt tagtactatt tatgcaagag
    30301 tttgttaaga acttccaaaa cttccctgtg cttggagtag ttttagttat ggcagttgcc
    30361 acaggagttt gtgaaaagac aggatttttt tcaactgcca taaaaatgag tttatcaaaa
    30421 gttaagggga atgcggttgt atttataata gcttttatag gagtatttgc taatcaagct
    30481 ggtgatgctg cttttgtatt agtaccaact cttgcaggag caatatttta tggattaaat
    30541 agaaatccat tagctggtat attctgtgga tttgcatcag ttggtggtgg atttgcaaca
    30601 gctgtaattc ctgggggatg ggacgttacg ctaacgccaa taactgtatc agctgctcaa
    30661 actattcaac ctgcatttga tatgacatta ttagctagtt attatgcact atttgtatct
    30721 gcgtttatag tagctacagt atcaacaata gtaacggtta aatttataga acctaaatta
    30781 ggtaagtata ctggtaaacc agaaggtata gatgataatc aaggttttga ggtcacaaaa
    30841 gaacaagcaa aagcagcaaa gttagcaggt tttacagttt tagcttatgt agcgttatta
    30901 attgctttgt gtataccagc taatagtttt ttaagaagtc cagaaggttc tttaatattt
    30961 ggagcaccac ttatgtcttg cttacagttt tttatagtaa tactattctt cttgccaggg
    31021 attgtgtatg gaatgagggt tggtaaaatc aagactgtaa aagacttaaa tgatatatta
    31081 tgtcaatcaa tggcagcaat ggcaccgttt atcgtacttg caatagttat aggtcaattc
    31141 ttaacgctat tctcagtgtc taatttagca caggtactag ctataaaagg tggagcatta
    31201 ctcaactcat tgccactacc accacaagct ataatactgc tattcttagt actagtagcg
    31261 tttataaata tatttgttat aagcggaagt acaaaatgga tgatatttgg accagtattt
    31321 gttccaatgt taatgcaatt aaacatacat ccagcattta ctcagtttgt atatagatta
    31381 ggagattctg taaccaatca tttatcacca ttaaatgcat tctttataat acttttagca
    31441 acagtccaaa aatacgataa aaatgcaggt atgggaacta tattctcagc tatgatacct
    31501 tacactatag gttactttgt agtacttgca gttttagtag tagtttggat gacaataggt
    31561 ataccagtag gaataggtgg acaagtttgg ttataaaaca tatttaataa ttttgggagg
    31621 aaagaaaatg gaaacaactg aaataagata caattttgat gaagtaatag atagaagtgg
    31681 aacaaactgt cttaaatatg atcttaagag tcaatttatg ccagaatctc cagaagatgc
    31741 actaccacta tgggtagcag atatggattt tgcatgtgcc cctgagataa ttaatgcaat
    31801 gcatgaaaga atagacagaa aaatatttgg atatagctct caacaaactg atgattatta
    31861 tgaagctgta tgtggatggt ttaagagaag atttgattgg aaaatagaaa aagagaatat
    31921 attctttagc ccaggggttg tgcctgcgtt aggatttttc acacaaatat taacacaaaa
    31981 gggtgatggt atcataatag aaaagcctgt atactatcct tttgaagata aaataactaa
    32041 taatgaaaga aaagtagtta atagtccttt aaagtttgaa gatggtcatt attctatgga
    32101 ttatgatgga tttgaagaat tagctaaaga tgaaaataat aaggtgttta tattatgtag
    32161 tcctcataat ccagttggta gagtatggaa aaaagaagag cttagaaaaa tagtagacat
    32221 atgtaagaaa tatgatttgt ggataatatc tgatgaaata cattgtgata ttattcgtaa
    32281 aggtgaaaag catataccat tagaagtagc atgtcctgat tataaagata gaataataac
    32341 ttgtacagct ccaagtaaga gttttaactt agctggtatg caattatcaa atatagtaat
    32401 caataataag gaattacaaa aaaaatggat gcttgaaaca gattctaaat tagctatatt
    32461 cctacctaat ccattcgcaa tagttgctac taaagttgct tataatgatt gtgaagattg
    32521 gatgaatcaa gtaaatgagt atatagatgg taatattaaa tatgtagaag attttgttaa
    32581 tgagtatatg cctaaagtaa agataataaa atctcaagga acttacttag tttggttaga
    32641 ctttagaggg tatggattag atgcaaaaca gcttgaatat gtaatgctta acaaggcgaa
    32701 ggtattgcta gatgaaggat acatattcgg agaagaagga gcaggatttg aaagaataaa
    32761 tgttgcttca cctagaacaa ttatagaaga gtgtatgaat agaataaaat gtgcatttga
    32821 aggattataa tagtaatatc taaataatat atttgtgtat tttaaaaaaa gactatgcta
    32881 aaaaaatatt tcaaagcata gtcttttaaa gttttatgat gagtttaaaa aatttcaaaa
    32941 cgttactatt cacttatatg tttatattag aaataattcc acaatatact cattttctaa
    33001 agttgcactt ttatacacct tgcttcacca atatcatgtg taaccaaaat agtagactta
    33061 ttctcattct taataatact ataaacatca tcacaaacta ataatctagt ctgataatca
    33121 agagctgaaa aaggttcatc taaaagtaga atatctggat ttacagaaag tgttctaata
    33181 agcgcaactc tttgtctcat acctccagat aattcttttg gatacatact tctaaaatcc
    33241 cataatccat aagtctttaa aaggctttca actctatcta aagatttttt atcttttttg
    33301 ccttgaattt ccaatccaat agttatatta tccattatat ttctccattc aagcaaatta
    33361 tctttttgaa acatatatcc aatatttcca gtaatcttta tatcaccact agtcggtttt
    33421 aaaagattag taagaatatt taatattgta gatttaccac ttccagaagg accaagcaaa
    33481 gttaatattt ctccttcttt caagttaaaa ttgacatcct ttaatacttc aagctcgcct
    33541 tccttagtgt agaagttcat atttatattt ttaacttcta ctatgttgtc attcattatg
    33601 ccacccccta taacttttag ctcgtatata ttatgttatt aacaatatga cctatttgta
    33661 ccatttttca taaatataat ataaatattt tgtttattta cgtattttct tagtaacttg
    33721 tacgtataaa ttatagataa ataaattata aggtagggga taaatttgag taacataata
    33781 acgaatttaa tagagtcaat atttactaat ccaataacta tattgttttt acttgcaata
    33841 actgtgtatt ctgtttttaa aataatagaa aataacagag tatattcatt tgtaagttca
    33901 aaattagacg aaatcaatag agactataag acaagtgagt tttataaaaa ggtaagggat
    33961 gactactata catatagtaa agaaaatcct tatgcagacg taaatataac ttcatttata
    34021 gaggaagttg tatctgattt aaagcataat aatttgccat tgttagaaaa aattagaagt
    34081 ataaaaaatt catcttcaat aagtatatta cttggggttt taggtacgtt tgttggtctt
    34141 tctacaatgc ttttatgtgt tgatactaaa gacattataa attcacttcc ttcaactatt
    34201 agtagtatgc aaactgcatt tacaactagt atatttggag ttgtattttc tctaattata
    34261 ggttacttta ctaagataaa agattgtgaa catgttttga tacaaataat gttaaaatct
    34321 gaaaatttac taacatctga aataactcat tttaagtcag aaagaatgga tttaaaagta
    34381 gaagaggtaa aaaatacaat aaaacaaata agtaagtcta tagaagctat tgaaaaattt
    34441 gataaaatat ctaaagattt aaatgacttt aatgatgaat ttatcagtgg tatagaagct
    34501 ttaaaaagcc ttttagaagg ttctcaaagt tctattaaga catttgacca gagcgtaaga
    34561 aaattagata aacagt
//
LOCUS       DAYAOY010000039        34245 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYAOY010000039 DAYAOY010000000
VERSION     DAYAOY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.039"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(266..577)
                     /locus_tag="ACGI6B_001916"
     CDS             complement(266..577)
                     /locus_tag="ACGI6B_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751361.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(633..1226)
                     /locus_tag="ACGI6B_001917"
     CDS             complement(633..1226)
                     /locus_tag="ACGI6B_001917"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9751362.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            1790..2020
                     /locus_tag="ACGI6B_001918"
     CDS             1790..2020
                     /locus_tag="ACGI6B_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751363.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            2077..2727
                     /locus_tag="ACGI6B_001919"
     CDS             2077..2727
                     /locus_tag="ACGI6B_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFG9751364.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            2941..3612
                     /locus_tag="ACGI6B_001920"
     CDS             2941..3612
                     /locus_tag="ACGI6B_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751365.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            3609..4739
                     /locus_tag="ACGI6B_001921"
     CDS             3609..4739
                     /locus_tag="ACGI6B_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9751366.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            5040..5162
                     /locus_tag="ACGI6B_001922"
     CDS             5040..5162
                     /locus_tag="ACGI6B_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751367.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            5159..6004
                     /locus_tag="ACGI6B_001923"
     CDS             5159..6004
                     /locus_tag="ACGI6B_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751368.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            6004..7032
                     /locus_tag="ACGI6B_001924"
     CDS             6004..7032
                     /locus_tag="ACGI6B_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751369.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            7035..8048
                     /locus_tag="ACGI6B_001925"
     CDS             7035..8048
                     /locus_tag="ACGI6B_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751370.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(8565..9815)
                     /gene="cme"
                     /locus_tag="ACGI6B_001926"
     CDS             complement(8565..9815)
                     /gene="cme"
                     /locus_tag="ACGI6B_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HFG9751371.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(9901..10467)
                     /locus_tag="ACGI6B_001927"
     CDS             complement(9901..10467)
                     /locus_tag="ACGI6B_001927"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9751372.1"
                     /translation="MVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSNAE
                     EALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGYRV
                     YTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISVCD
                     KLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(10486..10647)
                     /locus_tag="ACGI6B_001928"
                     /pseudo
     CDS             complement(10486..10647)
                     /locus_tag="ACGI6B_001928"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            complement(10762..11784)
                     /locus_tag="ACGI6B_001929"
     CDS             complement(10762..11784)
                     /locus_tag="ACGI6B_001929"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFG9751373.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(11784..14204)
                     /locus_tag="ACGI6B_001930"
     CDS             complement(11784..14204)
                     /locus_tag="ACGI6B_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9751374.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(14290..15210)
                     /locus_tag="ACGI6B_001931"
     CDS             complement(14290..15210)
                     /locus_tag="ACGI6B_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9751375.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            15840..16766
                     /locus_tag="ACGI6B_001932"
     CDS             15840..16766
                     /locus_tag="ACGI6B_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFG9751376.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(16955..17575)
                     /locus_tag="ACGI6B_001933"
     CDS             complement(16955..17575)
                     /locus_tag="ACGI6B_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9751377.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17547..19130)
                     /locus_tag="ACGI6B_001934"
     CDS             complement(17547..19130)
                     /locus_tag="ACGI6B_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9751378.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(19274..21301)
                     /locus_tag="ACGI6B_001935"
     CDS             complement(19274..21301)
                     /locus_tag="ACGI6B_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9751379.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            complement(21314..22063)
                     /locus_tag="ACGI6B_001936"
     CDS             complement(21314..22063)
                     /locus_tag="ACGI6B_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751380.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            complement(22362..24014)
                     /locus_tag="ACGI6B_001937"
     CDS             complement(22362..24014)
                     /locus_tag="ACGI6B_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HFG9751381.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            24602..25723
                     /locus_tag="ACGI6B_001938"
     CDS             24602..25723
                     /locus_tag="ACGI6B_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9751382.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            25759..26172
                     /locus_tag="ACGI6B_001939"
     CDS             25759..26172
                     /locus_tag="ACGI6B_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9751383.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            26165..26704
                     /locus_tag="ACGI6B_001940"
     CDS             26165..26704
                     /locus_tag="ACGI6B_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9751384.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(26904..27389)
                     /locus_tag="ACGI6B_001941"
     CDS             complement(26904..27389)
                     /locus_tag="ACGI6B_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751385.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            28294..28671
                     /locus_tag="ACGI6B_001942"
     CDS             28294..28671
                     /locus_tag="ACGI6B_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9751386.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            29067..30872
                     /locus_tag="ACGI6B_001943"
     CDS             29067..30872
                     /locus_tag="ACGI6B_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751387.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILLIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            30949..31236
                     /locus_tag="ACGI6B_001944"
     CDS             30949..31236
                     /locus_tag="ACGI6B_001944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751388.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSM"
     gene            32111..33325
                     /gene="dpaL"
                     /locus_tag="ACGI6B_001945"
     CDS             32111..33325
                     /gene="dpaL"
                     /locus_tag="ACGI6B_001945"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFG9751389.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            33655..>34245
                     /locus_tag="ACGI6B_001946"
     CDS             33655..>34245
                     /locus_tag="ACGI6B_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9751390.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT        12627 a         4251 c         5325 g        12042 t
ORIGIN      
        1 atctaaagaa gtgtaataaa ctaatttagg aagtatagaa aattaattta aagaagtata
       61 ataaactaat ttaagaaata tagaaaatta atttaaagaa atacaataca ttaattttta
      121 agtaacatta aattaatgta agtaaaataa tgttcatatg aatatttaaa ggtgcatttt
      181 ctgaagctaa gtatatgttg tagagtgtta tgtctctcta tcctgtgaag tttaatagct
      241 caagaaaatg cactatttat gatgattatt ttgttttatt tccaattttg gaaataacta
      301 ttctaattaa atctaaaatt ataccataaa taagaaaaga aagtatagat gctatgtacc
      361 aataaatcga aattgaccct gtaattgttc tacatgggat ttgcatacct gaatccataa
      421 aaacaaaaaa actatcaact ctggaaataa tagacaaaat aggatttaaa cctattagaa
      481 caatactttt tgagtcttga ccaatttgat gcatataaat ctcaaataat gcaattaaaa
      541 aaaaccaaga tgtaaaagat ttaataattt ttttcatcaa aatacctcac ataatataaa
      601 ttctgtttgc actatttgct tactacaact atttatctcc attcagtcac atcacttaat
      661 tgtaatctag gtcttcttga tggttcttca tcaggataac ctaaagatat tgctgccact
      721 aattgactat ctgtatttaa ccattcacat aattcacgat atgcaaagta tacatcacaa
      781 atccaaagac tacctagacc taattccaca gctgtaagcg acatattctg aattgcagct
      841 ccaattgatt gcatatttgc catttcatag aatcgttctt caggggatag cttttctaaa
      901 ggtgatttac ctaatatatt taaaataaaa attgttactg gagcttgttt catgatttca
      961 actgtgtaat ttgcaccagc gatatgttga cgacttcctg gaagaagccc attatcatta
     1021 atttcatttt gaataccttt gctcatggct ttaagcatag attctttttc cttttcagtg
     1081 atgacaacaa aacgccatgg ttgcctgttt ttagatgaag gtgcatttat tccagcttca
     1141 attattttct ctatagtttc atgtgaaatg ctttgatttt tatattttct gatacttcgt
     1201 ctttttgata tagaatcact tatcatttta agtaacctcc gtttattttt tggaaaaatc
     1261 tatttaattt ttattttcta ttttaattat tttagcatta atcagaaaaa aagtcattga
     1321 ttttagtata caattattaa tgattatatt aagaattgta tcaaaaagta tgtgattata
     1381 ctaagggatt atattaaaca ttatatttaa aattgtatga aaggttgtat ttaatattat
     1441 attgagagat atatatatta ggatttatat atattcagat ttattattat attgagtctt
     1501 atattaaatt aaggactata atttgaaaat aagctataaa aactcaatat tgttttgttc
     1561 aaagataaaa aaagaatact ttaggtgatt tttattaaaa attaagagtg ataagattat
     1621 atactttata aatgtaaata tgacggataa aaatgataaa tataataata tatatattta
     1681 cattaggagt tttctatact ataattattt aaaactaaat ataaatgttg gaattattta
     1741 ggacttgaaa ttgtctaaaa ataaatataa gtgatgaaga ggtgatgtga tgagattaga
     1801 tatggatagt atgttatatg tactaattta tatgacatta ttggtatcta tcattggaag
     1861 tatattttat ttttgtaaag acctcttaaa tcataaaatt aagtatgttt taataagaga
     1921 catatttgta aatagaatta catattctga agaacggttg cctaaacagt atatagtttt
     1981 tcagaaatat gatatttgga ggtattgtag tttgttttaa tttgaaatga atctattata
     2041 aattctaaag tttaaaaata aaaaggagag aataaaatgg atttcatttc aagtatattg
     2101 caaggattat taagtatatt atttaatttt acaggtgatt ttggaatttc tattgtactt
     2161 ataactttaa tagttaaatt agtacttatg ccactatcat taaaacaaag attttctgta
     2221 aaaaaacagc aagaattagc tgaaaaaatg gaacatataa aagaaaagta taagaatgat
     2281 actaaagaat tggaaaagca aatacaagta agttctgtag aaagtatgaa gagtatgctg
     2341 ggatgtctta tagttttggt tcaaatgcca gtagtatatg ctttatatca tgtatgttta
     2401 gatatgacaa aggaatttac aacaattata attccatgga ttgctaattt aggtacattt
     2461 gataatttgt ttatattacc ttgtatatat acattaatta tgttagcttc aaatttagtg
     2521 aattatatac catatttaaa aataaattct caagtaaagt ttaacaaaca aatggctgtg
     2581 gtgactacca taacaagtct tatactaact gttagaactc cagtagccat aggtctgtac
     2641 tttataacaa gtgcaattta ttcattaatt gaagatattt gttttagaat atattttaat
     2701 cgaaaaaata gaatagtgac aaaataatat aattattatt agaaatattt tagcctgcta
     2761 acatagaaaa agttagtagg ctatttttat ctatgatatc aattccatta agaatagtct
     2821 aaaaaatact atcaaaatgc tataaaaaat actatcaaaa tactataaaa aaatagttga
     2881 aaaataaata aattaatata aactaaaata aagcctacaa aaaagaggtg ataaatagaa
     2941 atggctaaaa tactaattgt agaagatgaa aaaaggatgc aaagtattat tgtagaatat
     3001 atgcaaaagg gaggatatac ttgtataact gctgatgatg gagtcgaggc acttacaata
     3061 ttaaagagca acaatataga tttaatgata ttggatatta tgatgccgta tttagatgga
     3121 ttttcagtgt gtagagtttc acgtgaaatg actaatatac ctattattat attgacagct
     3181 aaaggtgaag aggaggacaa attaaagggt tatgaatatg gcgcagatga ttatataaca
     3241 aaaccattta gccccaaagt acttttagca aaagttaatg ccttgttacg tagatacact
     3301 actgatattc ccaaaaattc attgagttta ggaaagattt ttattatggt agcatcaagg
     3361 caagtgtatg tagaagataa attgattgat ttaacatata aagaatttga attattgcgt
     3421 ttatttatgg aaaaccctaa tcaagttttt tccagagaga aacttttaaa ttgtatatgg
     3481 ggatatgatt ttgaaggaaa cacacgaact gtagatacac atataaaaac cttacgcaaa
     3541 aagcttggta gtgaaggaca ccatatagtt acattaattc gttcaggata taagtttgag
     3601 gttaaggaat gaatagttta agaaagatta tagaaaaact gaaaaatgta gatatttcaa
     3661 attggattaa acaaaatttt ggactatatt cagttagaaa aaaagtgttt ttactttcta
     3721 aattatcagg agcgttaatt atattatttt atctggttgt tgaagaattt tcgataaact
     3781 ctaaattaga attttggatt tggtttataa tactggttat atttattatg agtattgatt
     3841 tcttattggg aaaatttatt tcagaaccaa tcacatctat aaataaatct gcaaaaagta
     3901 tgtcccaact tgatttttct aatccatgta ctgtaaatac taatgatgaa tttggagaac
     3961 tttctaggag cctgaataca atgtctacaa atctacaaca ggcattatca gacctagaaa
     4021 gtgcaaatat tcaacttgaa aaggatgtaa ataaggaaag aatgctattg gaacaaagaa
     4081 aggagttggt agatacaatt tcacatgaaa tgaagactcc acttggcatt atacgtgcgt
     4141 atacagaggg gcttatggat gaagttgatg aagaaaaaaa gaaaaattat atgaatgtta
     4201 taatagaaga aactgataga atgaacaata tgattgtatc attattagac ttatctgcat
     4261 tggaagcagg agtttctaaa cttaacccag agcgttttga ttttatagag tttgttgaaa
     4321 ctgtggcagg tcgtctttta attgatatac cagatgccaa tttttatttt acatatgatt
     4381 tgccagagta tgaagttttt gtagtggcag ataaaatgag aatggaacaa gtagtagaga
     4441 atctagttat taatgcaaag aagcatgttg tatataatgg taatttagat ttatcgatta
     4501 catgtgaaaa tggcttgctt ttatttgaaa tttataatga tggtaggttt atagagcaag
     4561 atgagatttc aaagatttgg tctaagtttt atcgtagtgt tcaatctcag cgtacaggag
     4621 gttcaggtct agggcttgca attgtttcac aaatacttac aatgcaaggg ttagaatatg
     4681 gagtgaataa tcgtgataga ggagtagagt tttattttat gattccaatt gatgaataat
     4741 ttagtattaa tttataaatt ataagtatct gatgtaaaat atttttcttt gtatatgtaa
     4801 gcatttaaag agaataaaat aaaaaaactt aatgaaaaaa gcttaatgaa aaagtctaat
     4861 aaaaagctta atgaaaagtc ttgtttttct gatgaaaaca agatttttta ttaagctttt
     4921 tattatttca cttcgatttc acaagagaga cactctgatt tcaccaaata gaaataaaat
     4981 aggttaaata aattaaaaaa tagaagcgta attaaataag gttacaaggg ggagtactta
     5041 tgaaatggta tacatggttg actattatat tgataatatt gctttctatt ccgcttaaaa
     5101 ttaaatttat aaaatggtgg gatagacgac aaaaaaatga taaaggaggt tatgatagat
     5161 gataaaagta ggagagttaa aattttctta tggaaaagat aaacagatat tgcatggact
     5221 taattttgaa gtaaaagaag gagaaatctt tggattcctt ggacctaatg gttcaggaaa
     5281 atccacaaca cagaaaatct taaatggtgt tttaaaaggt tacaatggtc acgtatccct
     5341 ttttggtaag gaagttaaag catacacaga atctttatat caaaagattg gtgttttatt
     5401 tgaatttcct tatttatata ccaatttaag tgcaattgat aatttagaat atttttcgtc
     5461 tttttatcct aaaaatcaaa ggagagatat atatgaactc ttagatttgt tagagtttaa
     5521 gaaagagttt ataaataagc ctgtatcatc ctactcaaaa ggtatgaagc aacgtattag
     5581 tatggcaaga gcattggtaa gtaatccaaa gttgttattt ttagatgagc ctactagtgg
     5641 acttgaccca tctggagctg tactatttcg taagattatt gaagaagaac gaaaaaaagg
     5701 aacaactatc tttttgacta ctcataatat gctagatgca gatttaatgt gtaacaatgt
     5761 ggcttttatt gcagatggaa agattatggt aatagataaa cccaaaaatt taaaaatgaa
     5821 aaatagcaat aataaagttg aagtggaatt tgtatataat ggaaatcgag atattgaaat
     5881 tgtagatata caagagttag aatctggaat aacttttaaa tatgatgaaa tcttaagtgt
     5941 acattcaaaa gaacctacac tagaagaagt ttttattaaa tacacaggaa ggatgttagt
     6001 gtaatgtact ctaaattttt taccttagta aaaaaagatt tttggatgat gatttatggt
     6061 aaattttttg tgctgacact tggctctctc atactttatt cgtgttatat taattttggt
     6121 tatgtaaagt ttatggatga agatagttat cacaataatg tatatctata taatccagag
     6181 aatataagca tgagtgcatc tccattaatt aatcaagttt cttctatagg tgaattaaaa
     6241 gcaaaacttg atgaagatac aagtgcagtt tgtattgata tgagttccag taagccgaaa
     6301 gtaattttac atgaaagtat taaaggagtt gacgattata gagctgacta tgcattgtca
     6361 ttattatcat cagaaaaaga ccacaatagc aaaatatcag acaacaatgc aataattcaa
     6421 attaatgata aggatacatc tgagataaaa caaagaaaag acatgacttg tgagatttta
     6481 ttctttgaaa ttgtagctat tggattttta gggattgcct ctttattatt taaagaaaag
     6541 cagatgggag taatgcgtat tactggtatt ttacctgtaa aaagaagtct ttttatatta
     6601 tcaaagatat ttgctttttt gatatgtgac ctatgtttta cagttctttt agtagtattt
     6661 aatattggat tttttgaagg aataaaaata cttccacaag tattagttca agctagtatt
     6721 ttatcaatta ttatgtcttt gattggaata ggatgctcaa tgttattgag agattttaag
     6781 caattttctc ttatatatct tgtggttgct gtattcataa ctacacctat atttctatct
     6841 gcaaatacat cttttgaaat agattggatt aaatatcatc ccttctatca tgtttatagt
     6901 cagcttaaaa atgcgttttt catgaatttt tcaaaagaat tagtttattt tgtagtgtgt
     6961 tctttgacaa taatagttct atttttactt gtaaaatata tttttaataa agagttggtt
     7021 agggagggat aatcttgata ggattacgtt accagttaaa gagtataaaa aaagataaaa
     7081 tgtgtataat ttccttcttt ttaccagttg ttatggcgat tttaataaat tttatgggaa
     7141 caattgatat atcttcatta ggagagatac gttttggtat agtgaaggat acacttcctg
     7201 tacaaacaga gcagtggttg aagaagtatg gtatggtaag tgtatatgac acaaaaatgc
     7261 atttaatacg tgaaattaag gatattaaaa cagacacaat tggagttgtt agagatggta
     7321 aagggataaa aactgtgctt tatggaaatg agattaatgc tataaaaaat gttggaaaga
     7381 cattgccaga gatgtttaga ctaaagggtg atttaaaaga tgttgaggtt acaatattgc
     7441 ctaaagatag tattttaaaa gagtttcaaa atgtttttat tgccatgatt ttaattactg
     7501 ctatgtttat gggatgcacc tttaatgcaa tgaatattat atctgaaaaa gaagatggaa
     7561 tatcatttat aaatgaagtt ttgccacaga gtagaagtca gtatattata caaaaagtat
     7621 ttttgggtct tttatgtggg tgtttatctt caatgataac tgtatttata tgtttggata
     7681 tttcaatatt agatatgata atgatgttgt tactcgttgt attttcagca tttatttcat
     7741 cacttatagg tttatttatt ggaaagttgt cagaaggtct tatggtagga attgtttata
     7801 ttaaaatagt aatgatttta tttataggta ctcctttgtt aatatatctt ctaggggtta
     7861 ataccagtgg tatagggaat ttatactata taatgccttc agttgctaca tttaatggta
     7921 ttatggagat tattaatggt aatgtcatta ttatcaaaga tattgtaatt ttggctgtac
     7981 attgtgtagt atggtttatg atatatatta ttttagataa aaaaagaaag ttatttttta
     8041 gtagataact cagtataatt ataattggga caatttaata ggtgatttaa tatactttgt
     8101 atagtgtaat ttatgtagtg caatttgtat actttaattt atgtagtgta atagttagtt
     8161 ttattttata attattcaat ataatttagt aagaagttca gtacaattag agctacttag
     8221 tggaaaactt aataggtact taaactatat aaatacttta ttttcctcca tttgtactat
     8281 aaatttaatt tatgatataa atggaggtat tattatatga tttgataaat ttcatcatta
     8341 taacctacaa gtaaaggtat tattatataa agttaataaa gatagtatca aaatattatt
     8401 tgctataaag agaggggctc aaaataaaga taaacctatt tttcaatgtt gaaatgataa
     8461 aatatatttg atttcatata aaaaattttc ttcataaatt aaaaagtcca ttttaagccc
     8521 cctcattttt aaccagataa atcgagttaa actatttaaa tatattactc ggattcattt
     8581 gacttagata ttccttgctt gtaaaattgt tttgaatagg ggatagccaa agcaaaaaat
     8641 aaaagtataa tccctgctat gattattaaa ttattaatag aaattacatc tgccattggt
     8701 ccgaaaatca tcataccaag aggaataaaa ccagtatata taataatagg taagctaaga
     8761 actcgaccta gcatttgtgg tgctacatgc tcttgcaaca tagtcatagc tgatgcttga
     8821 atgattggaa ttgaaaaatt cgtaaaaaac ataaatataa aaaattgcca agcttgtgtt
     8881 acaagaccta gaataattga aaaaatagca taggataata ttgctagaaa aaaggtctta
     8941 tttctatttt taaaaccacc aaaaactcct ataatcaatc cacctaaaac catacctaaa
     9001 aatccaatca tttcaataat agacaggtat atataaccct tgccaaatgt tcgttcaata
     9061 agaagtccta taagaaatcc tgatggaaca caaagaagca tgaatgctcc ataagtaaaa
     9121 agcacttttc taatgaactt atctgaaaaa cagtacttta tccctgatac catatctgca
     9181 aaaaatgaga ctttttcagt ttgttttggt atatcatgtt taggaatttt gatgaatatc
     9241 aataatacaa tagcaataat tgcagtaaat acatcaatta aaagaacttg atttatttta
     9301 ttaccaaatc caagtattgc agcagctaca ataggaacta taaattgtac tatagattgt
     9361 aggctactat ttataccatt aaaacgcatt aaatgttctt ctggtacaag ttgaggaatc
     9421 attgcgttta cagcaggtgt ttgtacacca cttcctagtg aacgtataac agatattact
     9481 gatatggcta ttaattcact tgttgtgcca tttgatgaca tcataaatag aattagaact
     9541 aatgtagata tagcaattgt tgaatctgat acaattatga gtacctttcg attgtagtag
     9601 tctgcccata caccagcaaa aaaagaaatt atcatttgag gtataaagga gcatacagtt
     9661 aaaactgtta cccatacacc tgatgatgtc tgaagtgtga cataccatat cattgccatt
     9721 tgtacaagtg atgagccaaa taaggaaata tattgacttg ctaaaaaaag aatgactttt
     9781 ttcttccatg tagaattagt atttttgttt tccatcctat tcctcctttg atttgtgtgt
     9841 tgaatttttc ttgctcttaa ggataacaca caaatcaaag tctggagtta gagtggaggg
     9901 ctaggaaaat tgttttttca taatttgaag atgagatttc atggtttttg cattatgttc
     9961 agcttctttc aatgaagtta tcagtttgtc acacactgag ataatttcgt catcatcttt
    10021 tggagtgtca aggacagttt taatattagt attaggatta gaagataaag catttaacat
    10081 tcgtaaaata gctgaaagag aatagtttgc acaacgaagt gcgcttataa ttttaagtcg
    10141 ttttatatct tcatcagtat atacacgata accattttgt tttcttttta cttttaacaa
    10201 accattcatt tcccaatttc ttaaagtatc tattgtaacg tttaagtgtt ctgcagtttc
    10261 ttttctagtt aaaaagagtt tatttgtttc tggagtattt ccatttaaaa tctgtgcaac
    10321 aatagtaagt gcttcctcgg cattagattt ttctatttct aattgttgca agtattgatt
    10381 tacaagacta atagccttat caaagtccct attcgctgat gttcttacaa tctcaagggc
    10441 atcttttctt aatccccttt gtaccacttc aattttcaag gctgtacgag caaatctaaa
    10501 ttgttctata tgaaaatcat taaagatacg ataaccattg gtattacgct taggtttagg
    10561 tattaagttt agttcttcat ataaacgtac tgtattagga tgtacaccaa ttaaatctgc
    10621 tacctctgat gtcgtgtaag tgtccattag tattcaactc cttttagata tatagtatta
    10681 gtcataataa caccaaacaa aagtctggtg ttataatgga gggtttggtt atctaagagt
    10741 aattaattta tgaaagagta attaatctat tatagaagta agtatatatt tttcaataaa
    10801 ctcccatcta tcacgaggtt taaacactat tttagatggt atggctatta ctaagtcata
    10861 ttgtggaata caacaaatag catttcctcc agctcccatt gctgaatacc acaaaacacc
    10921 atctttttta catagccacc acagataacc gtattcatta ctattcattt taactgattc
    10981 ttcaatccac gatttagata taatacgttt attattccaa ataccatcat ttaaatacag
    11041 aaaaccaaat cgcaacatat ctctagcact caatgtaagt ccccagccac ctatagaata
    11101 accctgtcta tcttttgccc aacccttaag attttttcca aagaaatctt caaaaccaaa
    11161 atctttcata tcataatctg gtataacttt cataccaata ggtttaaata agtattcatt
    11221 agcaaattca cgagcagatt ttccagtagt ttttgttata atagctgaaa gtaggtgcgt
    11281 tccagctgaa gaatatttga aagtaccaat ttcgcctttt tgaccaagtt gattcaaggt
    11341 atattttatc caatcttttt gtttgcaaag tctttctaaa gactcagtca tatttttaaa
    11401 agggtatggt gcagtcattg taagtaggtg acgtatagtt actgcttttt tacatatttc
    11461 agaaggacta taagtatatt cagggaaaaa atcaacaacc ttttggtcaa catcttttat
    11521 atatccttta tctattgcaa tacctattaa ggctgatata atactttttg taactgatgc
    11581 aacatgaaaa gtatcatcag cattatatcc attatagtac ttttcaaaga caacttttcc
    11641 ttttcttgca ataattattc catttatatt actatattgt gagattatta aaggttcaag
    11701 ttgtgagagt ttttctgaac tcatgccaac acttgaagga tttacttttt caaatattaa
    11761 atctaagcaa ttgtttttat tcattaaaga tacctccctg ttatagattt tggattttct
    11821 tttagagttt ttggagaacc ttctgcaatt atataacctc ctttgtcacc accttctgga
    11881 ccaagctcaa caatccagtc acaaacattt aatacatcta tatcatgttc aacaacaatt
    11941 acagaatttc cattttcaac caattcatct aataattgaa tcaacttaga agtatcgtat
    12001 agacttagac cagtagttgg ctcatccaat acataaagga tattaccatt tcgtttttta
    12061 ccaatttctt ttgcaagttt gattctttgg gattcaccac cacttaaagt tggtgtaggt
    12121 tggcctaatt ttatataccc catacctatt tgttctaata catttaagtg tgaaacaata
    12181 tttggaatat ccttgaagaa atgtacagat tcagaaacac tcatatttaa aatatcatgt
    12241 atatttttat ttttatattt aatagataaa gattcatcat taaatctttt tccctgacat
    12301 tcactgcata tatggtctat acttagatta tttcccaacc agaccttttc atatccacta
    12361 ccactacatt taggacaagc ccctttggag ttaaatgaga aatgaccagc tgtaaatcct
    12421 cgttctaacg attctggctg acctgcaaat agtgtacgta tcttatccca tataccaata
    12481 taagaagcag gagttgagtt catatttttg ccaataggag attgagatat ctctgaatat
    12541 ccagaaatat gtttagtacc aatcagtcta tctgcaatag tttcaacaac tgcataatcg
    12601 tcttcctcac ctatttcact ctctgaagaa tttatttgat tattgatagt atggttatta
    12661 aattggcttc taagaagagg aagtagggtg tctgatataa gtgaactctt gccactacct
    12721 gattttccag atattccaac catagcaccc aaaggtaatg atacagttac atttttaaga
    12781 ttatttgtat ttgcattttg aatagttata caaggtattt tatcagtatg tgtaatgttt
    12841 ttattgcttg ttctattagg cattggatat tttccagata gatactgtcc agtgagtgat
    12901 ttgtcggatt gaagtagacc ttctaaatct ccttgatata ctaattgacc accttcaaca
    12961 ccagcttttg gaccaatatc aataatatgt tctgccattt taattgtgtt tttatcatgc
    13021 tcaactacta tgaccgtatt gcctaaatct ttaagtttct ttattgattt taagatgtct
    13081 attttttctg attcatgcaa tccagctgtt ggttcatcaa gcacataaat taatgagtcc
    13141 atctctgaat cgagatgtga atttaaaaat agtctttgca tttccccacc actcaaagtt
    13201 gacatttctc ggtataagga tagatgacct aatcgtgatt ctattaaatg ttgagttttg
    13261 gataaaatat catttacaag atttgtacca aatgtgttta aattaccttg atttattaag
    13321 agtttaagga aatcatcaat ttctaaaatc gtcatttttc caagttctcc aatatgtttt
    13381 ccgtttaaaa gtactctacg tgcttcttca ccaattttaa aaccattgca atctgaacag
    13441 gcagtcatag tataaactcc atttaaatct tcgccccttt tgtaacgatt gtgaaatacc
    13501 tttgataaag aggaactttt tttaccattt tcataatgac catagagtac ttcatttctt
    13561 acttcttcag gtagttgtaa gaattgtatg tctaaataat cattgaattt tttagaaaat
    13621 actctcaaat atccaggtgt aatctttgct ctatctaaga cttgttttag ggtatcatgc
    13681 ttatcaagga ctaatttttc aatattaatc tcaaaatatg caccatgtcc agagcacttc
    13741 atacacattc catctgaaga gttgtatgag aaataacttg gtcttaatcg ttcttcctca
    13801 tatccacaat tattgcaaat tagattatag cttacaggag tatcacacat agtacacatt
    13861 atttgacctt ccactgaata caatagtgta agcatgttga gaatattggt tcttgtgcct
    13921 actgttgaac gaggattgct ctggcgaata atattttgtt gaacagctat agtgggagct
    13981 aaaccttgaa tattatcaaa cttatcttca ctatcaatac ctgcaagtat acctaaagat
    14041 tgcagatatt gtttacgccc ttcctcaaat acaatgtcaa acataaggct agacttacct
    14101 gagccactga cacctgttgc aacaacaagc ttatctttgg ggatagaaac atctatattt
    14161 ttaagattgt gtatctttgc tccattaatt tttatttctt tcattggttc acctcatttt
    14221 atcaatttta tcaaaccgta aaatagggat atattaatcc ttaatacaag aatagattag
    14281 gtgaggttat taaaaaggtt ctataggtaa atatatatcg accaagtgtc tattttgagg
    14341 gtgattgtgt gggttgttaa gatatacttc aaaagggaag gaggcacgta gcttataatt
    14401 accatttgat agccattcat tatacataaa atcccacgca tcactatatt cagattttga
    14461 aatatcaaaa tgtcccacaa gatattttcc agaaggtatt atcatattgc caagattgtc
    14521 attctctttg acaacaatgt tatctggaat ggtcatgcaa atactagtcc ttaattgatg
    14581 ctcctgtgta aattgtggat tatcatgata aatggctaat atttgtgtgt tttcaagtcc
    14641 aagtagattc tgctcacttg catatgtgaa taaccgttct aaaagttttg ggaaagtact
    14701 ggttagatta ccatatgaac cagtatacct agtgtaaata gtattcatat tatcaagttc
    14761 tagaatttca atttcacctt taacctctct agtattctta catttaggtt ttgatatgct
    14821 tctattgtac tgagaaattt tatatggttc tttgcaattc ttgctataat tgtttctata
    14881 ttttataggg cttattttat agtaattttt aaatgctctt gagaaaactg ctgaatcagt
    14941 aaagccaaag tagtgtgcaa tatcagtaac agtcatgtca gttctatgaa taagaaaagc
    15001 agttgcattt tctagtttta gcctatttac atattgtgcc aaagtttctt tttctattgc
    15061 tttgaagatt ctatgaaaat gaaattttga aaaacctgct atattagaaa gttcatcaag
    15121 agaaagaggt tcatgtaaat gttcttctat atagtcttgc actttgcaaa tacgatttag
    15181 atattctttt ttacttttag catttgtcat atatagccct ccttattgtg taaaatgtgt
    15241 atcaattaat ttatagcaat atagtaataa aattttatgt ttttgtaatt tatgataaat
    15301 aagatagcgt ttaatttttt ataattgata aaagatattt ataattaata aaagatattt
    15361 ataattaata aaagatataa gatgccttat atttgtcttt gcatataagt atagtcttcg
    15421 taataattag tataccatag tgatttattt ttaattgtgt atttatataa atacacaact
    15481 tatgatatgg acgaattatt atgcaaataa gctgtaatta aataaattgc aatataaacc
    15541 agttgaaaca taagttaggg aaatattatc aaagttatta cttagcataa gagtatagat
    15601 ggaataaaat aagattaaaa aattacatac taaatttaat aaaattttta tattaaaaat
    15661 aatattaagt ttattgttta aataaaaaag ttaaatctac actattaact tattatactt
    15721 aaaactttaa aatgaaaata tcaaagaata ctttttaata aaaataatca tttaataagt
    15781 tatttaaaaa tctaaaaact ttacaacaaa ttatctattt aaattggagg agtgggaata
    15841 tgagtaatag tttttatcaa ttaaattcag cagtattata ttttaatgaa aaaagagatg
    15901 taaatagaat aaggtttaat cactatgtaa tgagaaaaga aaatatacaa agattaaaag
    15961 gaactctgcc aagaggacaa tttatgatga cagtaaggaa agctgcatct gaccttgatg
    16021 tatctatatc tacaataagt agacttgtaa atgaatttat agatttaggt atattgagat
    16081 taataagtag gggagtaaag ggaaattgtt gttctgtata tagttatgtt tgttctgaaa
    16141 gtagtaatgc agaaattgta acaaagaatg gtgatatttt caaaaatata gagaaataca
    16201 ttgatgcaac tattaacaat aaccaaaaaa caatgaaata taaacataga aatgagttgt
    16261 ttaagaacag taataccaac atatacagcc gtataaatca agagagtata gacaaagatt
    16321 atgatttgat aattcatact gggtatgaaa cagaaaatga gacaaagaaa aaagaattat
    16381 taaaaaataa tttaaataaa aagaatttaa ataaaaatac ctttgaaaat tcggatgaag
    16441 atttgactga agattctact tacaaaaatc cagaaagcat acatgaaatt attataagaa
    16501 aattaaatag tgaatcagga aaaaatttta aagctgattt gagtataact aaggcactta
    16561 tagataaaag attaaaagaa gggtatagtt tagaagattt ttacaaggtt atagaggcaa
    16621 aggtaagaag atggagatat acagatatgg aaatatatat agaaccaaca attctgtttg
    16681 ggtatgaatt tgagtcgtat ttaaatgagg agagttcttt atctgcttgt aattatggtg
    16741 atatgtgtta tgaagatatg tattgagata ttagttgctt tttagttgtt taaaatatgt
    16801 ttatatataa atataaatag tgtattaaaa tggagttgtc tcaaaataga gagaagcctc
    16861 ttcttaattg tttaaatgat gaaaatatat ttgatttaat acaaaggaga gtgctttatg
    16921 agttagaaga tttattttga gacactcact ttttttaata tactggagaa atataaccca
    16981 ttttaatagc ttttgtaata gcctcaactg atgatgaaac atctagcttg tcatatatgt
    17041 gttgtaaatg atttgataat gttctttcac ttatatacaa ttcatcagca attatttttc
    17101 tttttttacc actacagata agctgtaaaa ttcttttctc catagtagtt aaatcttcaa
    17161 taattacttc agtattttca gatggaaaac aagttacccc ttgatagact tttaataatg
    17221 aatcaattaa actatcaggg ctaatattct tattgaggaa tccatagata cctatttttt
    17281 tagcctcatg tttataaact gggaggtcgt atccagtcaa gattattatt ttaatagttg
    17341 aattagtctc taataaatta taagctactt ttaagccatc acattcactt aagcctctaa
    17401 ggtttatatc cattaagaca atgtctattt ttttatcttg aatcatttct gaaagattat
    17461 ggatgtcctg tgtggaataa aactgttcga tttctttgta ttcttctagt gcaattgaca
    17521 agcttttagc aaaaagcata tggtcatcaa ctaacaaaat atttataaga accatctcct
    17581 ttcataggga ttgtaatctg tacacaaact ccatgaggta tattatcaga aacacttatc
    17641 gttcctccta gattttctac ttgttcttta attgatataa tccctttgtg ttttattgta
    17701 tcaaaagtaa ttttagttat atctaaaggc gttttaaaag atgtatttgc cacaccatta
    17761 tcactaatat ttaattttat tatatttttt tctagtgcta aagctatcca tgcatggcta
    17821 ccatttgaat gtttatacac atttgtcaat aattctttaa tcagtcgata tatcaatata
    17881 tcatatggtt caactaagaa taaagtatca gaacacttaa atgaaacaca aatatttttt
    17941 tgtggaaagg atgaagaaac actttcaatt aaattctgat aattttcttt tactgttaaa
    18001 ttttttaaaa taacaggatg atagtcttgc atttgcttcc ttatacatgt atttagattg
    18061 ttaagtgttt catatataat atcttgaaca tctgggcgat atgacttact catcatgttt
    18121 tttattgaaa gcaggttctg taatatgtca tcatgtaaaa aattagaaaa ctctgttttt
    18181 agttgttctt cttgttgtaa tttttctaat gcaaaagtat acttgctatt tttaactact
    18241 ttattttttt catctctaag gttaaactcc aaaataatgt tacagaggaa aatgagcata
    18301 aataaaacat ttatagctat taatattata gaaaaattcc cattggcaaa tagtacgacc
    18361 aagctaatta ctaaaatact tataaaaaga aatatagccc tttgttttat gttaaaaact
    18421 gttgaaatag gaaaactatc attttcttta agtgcaatac catgaatact cataaaaaat
    18481 accagtacag ttagatagat tccaaaatta ccaatatgat ttgatattct tagtgtaaaa
    18541 aaataataag taaggaatcc tataattgtt ataaatactg aaatcgatat aggttttttt
    18601 tcgcttttta tgacaaaaac aacccttttc aaatgataaa aaataatata gaaaaagctt
    18661 attatactaa caacaaactg tattccgtac atacaagcat acgttctgtt agaaataaat
    18721 gctcctacaa ttgcaccaat gcaggtgata agcaataaaa tgtctgttat cttacggaaa
    18781 ttgtatccac taccttgaaa caaaaataaa aatataaatt taatagagac atacactata
    18841 actgtgctta atattttaaa aataatagtg cctattggtg tggttacaaa tgataagagt
    18901 aaataccaac tgtctagtgc tagtaatcca caaaacaatg aaataatgtt attttctcgg
    18961 acattacaag aggttatata tgtgatgtct atgagtaata atgttgaaat tattaaggga
    19021 ataatgcttt gaatattcat ataagtttca gtgcttgtag atattcccat gaagagaagt
    19081 gatagaatgt agtttcctaa aaatagtatt tttaaattaa aatttgtcaa tcttatacac
    19141 ctccttatga gttaattata ttaattttat gactaaatca aacttaagaa tttatagttt
    19201 taaattatat caaatactta taaatgaagt ataccaaatc gtcacacctt taagcttgac
    19261 gatttggcaa atattatttt tttaatacaa ttttacaaca ggatttaata gtaagtatgt
    19321 aatatatcaa atatactaca gaagttaaga aaattgcaat aatcacaggt atgtattgtg
    19381 aaagtgaatt tccaagtaaa ttctgcatca aagcagtctt ataaaccaag gtagcaaata
    19441 tacagtccac tataccaagt aaaaacggaa tattaaaaaa tggcaatact tgttttttaa
    19501 taattttttt aatttgtata ttagtataac ccattttctg taaaatttta taatcagatg
    19561 ttgaatctga aattgctgat atattgttaa agtataaaat acttccaaca gcaataaaga
    19621 ataataccac aagaaatccg attaacagaa atgtagaact attaattgat aataaatcat
    19681 ttatcctgtg agaatatcct tgtaaataag ggcttccatc taaataatct gaaatattac
    19741 tatataaatt ttcattattt ttaattgatt taccattaat acttaaaatt cttgtcattg
    19801 gtaatttatt ttgtgctatt tgctcatata tactatcatt aacgataagt gttccaacgc
    19861 tattggcaaa agaaatagca ttgtttaatg atgtttccct tacagctaca ggtgtttctt
    19921 taccattaac aagtaatgga taaatcgttc cattttcact atatccatca ttatttggtt
    19981 gatatttcat taaaatacat tcattatccc ttaaaggggt aagttgtttt agtagctttg
    20041 ttcgattttg tgcctttaat aaagtataat aatcagaaaa agatatacat tcaaatcctg
    20101 attctcttga aataacttct ttagaagaac tactattgga taaattattc ttagatgtac
    20161 caatttcata ttcaaatgga aggtcttttg atgaagattt taccttataa atatttgtct
    20221 gtgtaaaaat aatatcatct gtattagacg tatattgact gacaatttgt tttacatctt
    20281 ctatttgttt atttttttct actctaaatt ctatctctga aggtgcaata cgcgaaacag
    20341 atgcaatagg gtagtataag gacagtgcca taacacttga aactgtcaaa gtagtagcag
    20401 agagtaatgt aagcataatt agagttttag agtttgaacg tatacgatag ataaagtttg
    20461 gaatagctat tatcttcata tctgtgtaga aagtacgttt atttttttta ctattttgca
    20521 taatatatgg aagaaaggaa ttgataaaga gaactgtacc aattacaatg ataatcatag
    20581 ttatcaagcc catttgataa aatccaatac ttacccatac tgatttagct ccacgaagaa
    20641 tatctagtga taaacaatac ccgcttaaaa tcataataaa ccccataaag gctggaattt
    20701 ttctgaactt taattttttt tctgcacttt tttcatatct tatcaatttt acaagtggag
    20761 tcttataaaa aaatttacca tttgatacag ctaagactaa aacaactaat gctataaaga
    20821 taattgtttt atttattgca tttatattaa aaaatggtat ctgtgaatta tcaattgata
    20881 aattaagtag tgctgtaatt ccaaagacaa tacccttatg tgccaatgct cctaatacca
    20941 atccaataat aaaagctcca ctacaaatta atacattttc aaaggtaagt agcgataata
    21001 ttgtaaactt acgatagcca agtaaagcat atatacctaa ttcttttgta cgacgtctca
    21061 agaagaatcg atttgagtat gccatataga agatgacaaa tgccatcaaa aatatcgaaa
    21121 taacattaca catagattca actctaccat cagttgaaat tttttctagc attatcttat
    21181 taacagaaaa tgaagtaaat gcgaaaaata cagtaataac aaaagagact gaaagtagat
    21241 atagagaata gaatgaaaag ttttttttaa gattctttat tgccgtataa aaaatgttca
    21301 ttaaattatc ctcctatctt ttagtatcca attttgcaat ttcttgaata atcaactcat
    21361 aaaatgtatg tctattattc tgttgtttat ttaattcttt aatgatatga ccatctttaa
    21421 aaatcaaaat tctatttgca aaacttgcta cataggcatc atgtgttacc attaaaattg
    21481 ttgttttcaa ctcctcattt gcttcctgta gtgagtggag tagttcagta gctgaattag
    21541 aatccaaagc tcctgttggt tcatctgcaa agagaatact aggatgtttt actattgctc
    21601 taactgctga agctctctgt ctttgcccac cagataattc atttgggtat ttatttaatt
    21661 gagaagctat gccaaatcgt tcagctagac tctttaccat gttttctatt ttatctggat
    21721 tgagtttctg aagtgtaaga ggtactgaaa tattctcgaa aattgtaaga ctatctagta
    21781 aaaaatactc ttgaaagata aagcctaaat tatccttacg aaaatcagca gcacaagtgt
    21841 cattaagcga tttcaaattt atattattta tgaaaatatc tccacttgtt ggtttatcaa
    21901 ttgtagataa tacattaagt agagttgttt tcccagagcc agaagctccc attataccta
    21961 taaattcacc ttctgataca ttaaaggata ccttatctaa actcggctgt tgccctttat
    22021 tataagtttt agttatgtta tttactttta atattgtatt cattgtatat gtctcctttc
    22081 gtcttaatat agtcaaatta tatattaacc ttattgtttc ttcatgagta aatcacgcaa
    22141 taattctaca caaattactt atatcacaat atattgttta aaatctaact ttattaggtt
    22201 tgggatgatg gacaagattt taatttaaaa gagtgcatag gtatatataa tggcataatg
    22261 tggatttaaa ttaaaaaaaa taaaataaag ccataaatat tgaaattttc aatatttatg
    22321 gctttattgc ttgtaataat taatagaata tatatttttt attaatcatc tatttcaaat
    22381 tctaatattt taccagtaat tgcattaatt gttatctcat attcagtttt tccttttatt
    22441 atttcacctt catattcttt gtcatcttca tcatacttaa attttactat tttcccacca
    22501 ggcactttat ctagcattat tgatttagct ttttcagcac taataataga actgttgtca
    22561 ttagtattag agttattctt atcattatca tcaaagtcat catcagaatc attgtccatg
    22621 tcaatatcta caattcttcc atcaaatgca cttatctcta attcatatgt tttcttattt
    22681 tttattactt tagcctcata ttcgttatct tcagcatcat atttacaata gattaaatta
    22741 ccattaggaa cagcatctaa tgctatagac ttagctttat ccaatgatat attttgtgta
    22801 cctaaattat tatcatttaa gtcatcatct ttttcaattt ctattatttt accagagtag
    22861 gcatcaattt ctatctcata taggatatta tcctttataa ctttgcagtc atattcatta
    22921 tcatcttcat cataataaca aactaaaata ttgcttcctg gaagagcttg ttttaatatt
    22981 tctttagcct gtattaaaga tattttttta gttttatttc ttatgttcac attttttagt
    23041 tttaaaatat cttcaaatgc tttttgatta tatcctcctc ctacttgtgt tatttcatct
    23101 atatctttat ttttaataaa ttctttttga ctatttacaa ggctaccatt agatatcatt
    23161 ataggagaat tttctttagc agctagtact cctattgata atgaatctac taatgcattt
    23221 tcttggttaa taccatcttt agctacaaat acattattta tttttttatc tttataaaat
    23281 ttatcaataa tttttgcatt agtattgttt ctatctgaac cactgattct ttctctgttt
    23341 ggatatttct ttactgtatt gtctgataaa atcttatttc ctccaactat gtaagatttt
    23401 tttatagacc tattgtttaa gaaatttata ctactatcat tgctgttttc atctgatact
    23461 aatattatag gcatattatg tattgctgct ggagaagcta tactaactgc atcagatagt
    23521 ccctttagtc catttacaac tgcaacattt tctacctttt ttatcttatc tatttcttta
    23581 agtacatttg tagacatttc atatatattg tttcctacta ttttatttat ttttatgtta
    23641 agtgatttta tttcattttc tactttagaa gaaatgctat ttccactatc tacaatgtac
    23701 acattatcta caccaagcct tttaagctca gccttagttg aattttcaat actgttgttg
    23761 tttactaaaa gtataggtgc attctttagc ttagcaaatg gatttgcaca aagcgcactt
    23821 tgaatagaat taccattaac tataatggct gtttttgatt gaacccatcc caatttactt
    23881 acttctactg ctgtttcata tcttgtttta cctattaatc tttcttgctt tgtagtattg
    23941 tctgcaaaaa ttgctgaaga actagaaaaa aacataatac ctgatattaa tatagctaaa
    24001 tttttcttat tcatattttt acctcacaat ctttaaaaat ttgttattta gcatacttaa
    24061 taaaatataa tcttaaaatc aaattgatgc tgagtttgaa gttacaaaat caacaaataa
    24121 gattttttta atacaaaatt ttccttttta tatttaacat tgtatcacaa atataaaaag
    24181 gaaaaaactt tttaaaaagg gtatacacta ttcatttttt atcttataaa taagtcttta
    24241 ccactgtttt atatatttta aaatagacac aagacctatt tctaaatctt ttaggtgaaa
    24301 tttcttttta aacatatcta aaaagtattt gtagtaatat taatggactg aatttcttaa
    24361 agtatatatt tctttacatg ttggagtttg tgaagtttga gaattggagg gtgaaagaaa
    24421 catgtaatgg attaatatta aagaactgta tttttataat atttgtctta aaaataaaaa
    24481 aatctattga cttgaagtta actccaagta gtaaagtata attatacaag aattatatac
    24541 ttgtaagaat agttaaagta gagagaaaaa acaaacaagt tttaaagggg gaagtaaaac
    24601 aatggaggca aaaaaattag gatttggatt aatgcgttta ccaataaaag atgaaaatga
    24661 tgttacaact attgatatgg aacatttaaa taaaatggta gacacttttt tagaaagggg
    24721 atttacatat tttgatacag cctatgtgta tcacatggga aaaagtgaga tagcacttag
    24781 agaatctcta gtaaaaagac acaaaagaga gagttttact atagctacaa aacttccatt
    24841 aatggcttta aagaaaaaag aagagcaaga aaccattttt aatgagcaat tagaaaaatg
    24901 tggtgtagac tattttgact actatctttt acacaatata ggcgtgtcac attatgaagt
    24961 agctaaaaaa tttgatagct ttaagtttat tgaagaaaag aaaaaagaag gaaaaataaa
    25021 gaatattggt ttttcttttc atgatagtgc agaattattg gataaagtct tgagtgagca
    25081 tccagaagta gacttcgtgc aattacagat taattattta gattgggata atgaaagcat
    25141 acaatctaga aaatgctacg aggtggcaga gaagcataat aagcctgtaa ttgttatgga
    25201 gccagtaaaa ggaggtacac ttgcaaagat accagaaaaa gcagagaaat tattgaatga
    25261 ctacaatcca gatatgtcaa tttcatcttg ggcaatacgt tttgcagcaa gtaaagataa
    25321 tgttatgatg gtcttaagtg ggatgtcaaa tatggaacaa ttattagaca atacaggata
    25381 tatgcaaaat ttcaaaccat tcgtacaaga ggaatataat attatagatg aagcagtaga
    25441 gattatcaac gaatcaattt tagttccttg tactgcctgt cagtattgtg tagaaggctg
    25501 tccaaaaaat atcgctattc caaactattt tgcgttgtac aatgcagaaa aacaagctct
    25561 taatactgga ttttcaacac agatggtcta ctataataat tatactaaaa cttatggaaa
    25621 ggcctcagat tgtattgaat gtaaacaatg tgaagaaagc tgtcctcaac acattaaaat
    25681 tatagatgct ttaaagaatg tagcagaaac ctttgagaaa taattcaatc tatacaatat
    25741 caagaggaga agatatgtat gacgatttca gaagttagta aaaaatacga gctttcagcg
    25801 gatactttac gatattatga acgaattggt ttaataccac ctgtaaatag aaataaaagt
    25861 ggaattaggt cttttacaga agaagattgt gagtgggtta atttcataaa gtgtatgaga
    25921 ggtgcaggtc tttctattga gacattaatt gagtatgtag ctatgtttca acaagggagc
    25981 agtactatca aagctagaaa agaactttta atagagcaga gaaaccaact agcaaaaaga
    26041 atagaagaaa tgcaaaaaac tttggaacga ttaaatttta agattgatag atatgaagaa
    26101 ggtattattg agaaagaaaa agtattaaag agcagtagaa ataagaaaat ggaggcagta
    26161 tcttatgagt aaaaatgtat taatagtatc agctagtcct agaaaaggtg gaaattctga
    26221 tttattgtgt gaccagttta tacaaggggt attagaatct aaaaatcaag tggagaagat
    26281 atttttaaaa gataaagata ttaactattg tactggatgt ggtgcttgtt atgaaaaaga
    26341 agcaaattgt tcacagacag atgacatggg agaaattctt gaaaaaatga ttgaggcaga
    26401 tgtgattgtt ttagcgactc cagtttattt ttatactatg aatgcgcaaa tgaaaactat
    26461 gattgataga acttgctcta gatatactga aatttgtgat aaagatttct attttatcgt
    26521 tacagcagca gatgaaaatg tggctgatat ggaaagaaca atagaaggat ttagaggatt
    26581 tacatcatgt ctaaatggtt ctaaggaaaa aggtatagta tatggtgtag gtgcttggaa
    26641 tgtaggtgat attaagggaa gtaaggcaat gaaacaggca tatgaaatgg gtaagttggc
    26701 gtagtatgtt tgtataaaat tgtataaaat tgaatatatt cttatcattt caacaataaa
    26761 aagaggctta tctgtatttt gagacagcct ctttgtgcta tatcatttat ttttattcta
    26821 tatccttatt atacccttta tttttttatg cctatttaag tatctgttgt tactcatata
    26881 ttttaataat tacccttaaa aaactataca gattcctgca taatattatt ttctttagta
    26941 attaattttt ctaggttttt ccaatccatt tcttccatat ttgcaaaata atgaccactt
    27001 cctatgcaag tcaggtaaga ctttccatct aattcaaacg aagaacctcc aatacacttt
    27061 gaattacatt tcttacaagt acgagtttta gttatactat ctttaatagt tttagagcat
    27121 tcctccacag catctttata tgtatttaga tgaaaaagat ttgctttaac tctccatttt
    27181 ttttcattac attcaatagt aaataagcta cgttttggtt tcttagttga atatactatt
    27241 ttgtaagaac gagcacctgt ttttggttca attttacaat ctaatgattt accaagttca
    27301 tgtaaatgac atgcaatatc ataatcttta ccacttagta cttctaacat ttcttctgtt
    27361 gtttctgctg ttaattcact tttgctcata ttaaaaaaat ccccctatat aaataataat
    27421 ttcatatgaa aataattaat ataagtatat taaataaaac tattaaaatc aataaaatta
    27481 ctgcaatctt acattattgg gcatagaatg agagctaatg atttattaga aataagactt
    27541 aatagtatga aaatttactt agacgtggta caattctata aaagatttaa aactaggaaa
    27601 ggagaaagtt gttgagatac ggccaatata acaatttatt tataagttgt aaatttatta
    27661 tgagattaat tagattgtta agtaatacta tcaaaaggta gttttttgga ttttcagcaa
    27721 cagtggtaga tattgataat tttttggtta ttattatatt tagctgttat attttcaata
    27781 ggctctatga taactagggt aaataaggta tgggtaaagg ttttattatt tataatagtg
    27841 ggattgtcaa ctatagttct ttcatatgta ataacttgat tatgcaaaat tctatgaagc
    27901 aggaaccctg tgactttagt catgggaggt tcagaattta aggttaaaaa ataactttaa
    27961 tctgtaaatt ctcaaaacaa gaattctcat gttggtaaat ttctagatat gagaaaaaaa
    28021 ttgacatcat aaaaaagtaa ttttagagcg tttttttacg aaaaaattca gaagtaacta
    28081 ttttgaatga cttcacaaaa ttttatatat aaataaagtt tggcacgtta attgctttaa
    28141 taaaagttaa caagacacaa ataaacataa tcatctaagt attgattatt tgaagtttta
    28201 gatttgaagt ttaatctaaa ggttcgtatt aatattggtg tttaggaaat tttattttcc
    28261 tgtaaatata acttaataat aggaggtgaa gtcatgtcaa aagtagtaat agctacagag
    28321 aaagctccag gagcaattgg accatattct caagcaattc aagttggaaa catagtttct
    28381 acatcaggtc aacttccaat agatatgaag actggcgagt tagtatctga cccaaaagaa
    28441 gcaacaaaag cttctttaga taatgtaaaa gcaatattag aacaagctgg tacaagtatg
    28501 gataaaatat ttaagactac agtattcgta aaagatttaa aggattttaa tgatattaat
    28561 gaagtatatg ctacatattt taaagaaaat ccaccagcaa ggtcatgtgt acaagttgct
    28621 caattaccaa aagatgcagt tttagaaatt gaagcaatgg cagtattata agtattataa
    28681 caataaaacc aataacaaaa ctaataaaaa ttaaaaacta ataaaaaaag tctacaatta
    28741 aaaaagttta tattaaaaaa gtctatataa aaatctatct aaatatttaa ctattgagct
    28801 tttctcatat aatgtatatt tttatgtcaa aaatatattt gttatatgag gaaagctttt
    28861 ttttattatt gtattgtgat ttattttatt gtctgcatat tataattaag gttactatat
    28921 aaaacaaact ttttataaat taatattgtc tgttttaacg caaatatata aataagtaat
    28981 gttttatata tattttaaag agtgactata tgttaaaata tagctaaact gataatttgg
    29041 taaattaaat tctaatgggg gaaactatgt taagtaaatt aaaagaattt caacaagaaa
    29101 tgataaaata cactgaaaca gtagctagtg tgttagatgt agatattgaa atagttgacg
    29161 atagattaat aagaattagt ggtacaggtc tgtataaatc aaagataaat gaaagcgttg
    29221 ttactgaggg ctttatttat gacaatgtta tacagacagg tcaggaatta gtcgtattgg
    29281 atatatgtga taatcagctt tgtatagaat gttcccatta catgaaatgc ctaaataaag
    29341 taataatagc agttccaata aagtataata atcgaactat aggagtaatt ggtgctatta
    29401 gtacagataa aactaaaaaa gtagaaataa gtgcaaaaat agataattat ttaaaatttg
    29461 tcaatcatat atgcgattta atttctatga aaatagaaga acatgaagcg tcaaaaaata
    29521 gcagtagaaa aatggatatg atgatagaaa tcattgaaaa tgttgaaaaa ggcgttataa
    29581 tactagacat aaatagcaaa atatcttata taaacaatat agctctaaaa aaattagata
    29641 tagataaaaa tataatagaa aatatagtaa atatagtatc tgttgaatct tcctcaaatg
    29701 gacatgaatt actagaaatt gatattgata acaaaattta taatataaat gccaaaataa
    29761 ttccggtata tccatatata aatcaatacg acaaaataat aatatttgac aagacatata
    29821 tcaatcataa aggacatgga aaagtgaata gtggctgggg aaatagtgat atagagtcta
    29881 taattggaaa ttcagaagca atgttaaaag taaaagagag aactaagaaa ttagcaaagt
    29941 caaattcaac tgtacttata actggggaaa gtggtacggg taaagagcta attgctaggg
    30001 ctatacatgc tgaaggtagc agatggaaca agccatttat tgcgataaac tgtgctgcta
    30061 ttccagaaaa tctccttgaa agtgagctat ttggctatat aaaaggtgca tttagtggag
    30121 ctagtagtgg aggtaaagta ggtaaatttg aacttgcaaa tgaaggtgtt atatttcttg
    30181 atgaaatagg agatttgtca atgcctttac aagcaaagtt acttagagtt ttacaagaga
    30241 gaaaatttgc gagaattggg tcaaataaac ttatagattt agatataaga gtgattgctg
    30301 ctacaaataa aaacttattg aaattagtaa atgaaggtaa gtttagggat gatttatact
    30361 atagattgaa tgtaattcct ataaatttgc cacctcttag ggaaagaaag gatgatattg
    30421 aggcaataat gatgaagttt gcttctaaat attctttaga attaggtatt cagttaaata
    30481 aaatagaaga aaatgtaatg aatatgttga ttaactataa ttggccggga aatataaggg
    30541 aactggaaaa tgcagttgaa tatatgatga atttggttgg agatgatgga ataatatata
    30601 aagatatgct tccattagat attttaaact attataatat taatggaaat atttataaaa
    30661 ataaggatgt aaatataata tttgaagatg atatagttgg agggatagta gagaatcaag
    30721 aaaggatact tttaattaaa gaattagagt tgacttacat aaacaagttg ttgaataagt
    30781 atggaagaga tacaaagacg aagaagaaga tagcaaaaga cttaggcata ggtttggcta
    30841 cactatatag aaaattggaa gaggaacagt agtaatataa tattaaaatg ataggtattt
    30901 gtgtgttttt atagataggt atttttactt tatggtggag gtgttattat gcaatatgcg
    30961 atagagctgt attatgataa aaaaacggaa aagcaacttt ttgatttatc taaaaaaatc
    31021 gctgatgaaa aaattagtac gaagtattta gaatggaaaa caagacctca tcttactttg
    31081 gcttgtttca atgatgtgga tgaggcttgt tgtatagaca agctaaaggg gtttgcacaa
    31141 aaccataagg taatacctgc ttacattggt tcagtaggca tgtttaatga tactaaaacg
    31201 gtgtttgtat cacctataat gaatagcagt atgtagcagt ttcagagaga attatatgag
    31261 gtactacaag attttgattc aaatggttgg gaatggtacc accccaatcg ttgggtaccc
    31321 cattgcacaa tagcacttac tggagatgat gaaagtgagg ctttttttcg ggcaagtgat
    31381 gtattactgc gtgagttcaa aaaaataagt ggtgagtttg tctcaatagg attggttaaa
    31441 attacatttc ctgtggaaga aatttttaca gtgaatatga ataggtaact ttaactctgt
    31501 tgtggatgtt atgaatattt tgtatagtgc atttcatcag ataaatgcga agttgcgaat
    31561 ttcgacagct caatagtcgc aagtagggca caatggtaca catattgcac ttttgcttat
    31621 tggggggacg cccatgtaaa cttctgtaag aagctatgaa aagatttaag ccattttgga
    31681 ggctttgggt aataaagaat atttgggtaa taaagaatat aaagtatata aatttataga
    31741 gtttatgcgt aagaaggtta tagccatata tttatagaaa ataaaagtag taatatatct
    31801 gacaaaacaa tagacatata aaaaataaga gcataataaa ggaagaatta agataaataa
    31861 acgatttctc gaattgataa ttctcgtatt gataaaaatt tcccaatatg agaatttttt
    31921 cttttataaa tcttattttt cacactcaaa atttataaac tttaaagaaa ttttataaaa
    31981 gtatgtaatt ccaacataaa aaaattgttt ttagaataaa taatattggc acggtaattg
    32041 ctttgctata aggcacaaag ataaaaaatt aaaatgacac cgaaaaattt gaaaggggaa
    32101 tataaaataa atgagagaaa taaaatggaa attaaataac ttacctaaat cagaggaaaa
    32161 agaaaaagga attgaatttt taaaagatga agagattgca aaggctaaag cttttcatga
    32221 aagctttcca caatatgaaa aaactccttt agttaaatta gataacttag caaaatcact
    32281 aggcgtaagc ggaatatatg ttaaagacga atcttataga tttggtttaa acgcatttaa
    32341 agttttaggt ggttcttact ctatgggtag atacttagct caaagattag atacagatat
    32401 atctgagtta gggtatgata aattaacttc aaaagaaata aaagaaaaat taggagaaat
    32461 aactttcttt acagctacag atggaaatca tggtagagga gttgcttgga cagctaataa
    32521 attaggtcaa aaatctgttg tattaatgcc aaaaggttct tctgaattta gattaaataa
    32581 aatcaaagga gaaggtgcag atgctagtat aacagactta aactatgatg atgcagtaag
    32641 attagctaat gattatgcag aagctgatga ccatggagta atggttcaag atacagcatg
    32701 ggacggatat gaagaaatac cagcatggat aatgcaagga tatggaacta tggctaaaga
    32761 agctatagaa caattaaaag aatatggagt agatagacca acacacgtat ttgttcaagc
    32821 aggagttggt tcacttgctg gtgcagttca aggatatgtt gcttcaatat atgatgaatg
    32881 cccaataaca gtagtagtag aggctgatga ggctgactgt tactataaat cagcagaagc
    32941 tggagatgga aaaccaagat ttgttggagg agatatgcca actataatgg cagggcttgc
    33001 ttgtggagaa ccaaacacta taggttttga agtgttaaaa aattattcat cagcattcgt
    33061 ttcagcacca gactgggtat ctgctaaagg tatgagaata ttaggaaacc ctctaagagg
    33121 agatgagaaa gtaatctctg gagaatctgg agcagttaca actggagcat tagtttcaat
    33181 ccttgaaagt gaagatttaa aagacttaag agaagctttg aaactagatg agaactctaa
    33241 agtattatta ataagtacag aaggagatac tgaccctgat aaatatagag atatagtttg
    33301 gaatggtgaa tgccaaagta aataaaagac ttaaggaaaa gaaaatctta acaaataaat
    33361 gaaaaattta aggaaaataa aaacttaaat aagtgaaaac tttaaataaa ataaaaacta
    33421 aaacaaatga aaaatctaaa taaatgaaaa agtaaagcaa ataaaaaagt aaagcaaatg
    33481 aaaaattcaa aataaaaacc aaataaaatt aaaataaagt ctaaataaaa atttaataaa
    33541 taaaatataa agaaataaaa aatcaataga ttaagtgaaa tgctaataaa ttaaaaaaat
    33601 gaaatatcaa aataaaacgt caacaaatta aaaattatat gggggaatat caaaatgtta
    33661 aatgaaacaa gaaaaaaaca attaactgaa gtatgccaag acttaataag aaatgctagt
    33721 tattcaggtc aagaagaaaa cgtagtaaaa gctatagaag aaaattttgc tttactaggt
    33781 tttgatagtt ggtcaagaga tagatatgga aatatagtag gatgtataaa aggaaataaa
    33841 ccaggtaaaa aaatattatt tgacgggcat atagatacag ttccagttcc agatgcaagt
    33901 aaatggagtg ttcctccttt tgaagcgaga atagtagatg ggaaaatata tggtagagga
    33961 acttctgata tgaaggggca agtaagtgct atgatggctg ctgtttcata ctttgcagaa
    34021 gatactaaca aagactttga aggtgaattg tatgtagcag gtgttgttca tgaagaaata
    34081 tttgaaggtg tttcagctag agagataagt aaggctgttc aacctgatta tgttgtaata
    34141 ggtgaatctt cagaattaaa cctaaagata ggtcaaagag gtagaggaga aatagtagta
    34201 gaaacttttg gtaaaccagc tcactctgct aatccagaaa aaggt
//
LOCUS       DAYAOY010000040        33525 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYAOY010000040 DAYAOY010000000
VERSION     DAYAOY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.040"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1032
                     /gene="ytvI"
                     /locus_tag="ACGI6B_001947"
     CDS             <1..1032
                     /gene="ytvI"
                     /locus_tag="ACGI6B_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFG9751391.1"
                     /translation="FLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAIIALMINP
                     ISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNISANPEDIS
                     NTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLATSIPYII
                     IFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYIRAYTILM
                     AITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLIKNYFIAI
                     SMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFCLGLVCMH
                     DILKKVDIF"
     gene            1122..2348
                     /gene="pepT"
                     /locus_tag="ACGI6B_001948"
     CDS             1122..2348
                     /gene="pepT"
                     /locus_tag="ACGI6B_001948"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFG9751392.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2555..3607
                     /locus_tag="ACGI6B_001949"
     CDS             2555..3607
                     /locus_tag="ACGI6B_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HFG9751393.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(3942..4511)
                     /gene="yfcE"
                     /locus_tag="ACGI6B_001950"
     CDS             complement(3942..4511)
                     /gene="yfcE"
                     /locus_tag="ACGI6B_001950"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFG9751394.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(4536..5336)
                     /locus_tag="ACGI6B_001951"
     CDS             complement(4536..5336)
                     /locus_tag="ACGI6B_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HFG9751395.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            5471..6331
                     /locus_tag="ACGI6B_001952"
     CDS             5471..6331
                     /locus_tag="ACGI6B_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751396.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            6331..7017
                     /locus_tag="ACGI6B_001953"
     CDS             6331..7017
                     /locus_tag="ACGI6B_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9751397.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            7069..7611
                     /locus_tag="ACGI6B_001954"
     CDS             7069..7611
                     /locus_tag="ACGI6B_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751398.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            7690..8157
                     /locus_tag="ACGI6B_001955"
     CDS             7690..8157
                     /locus_tag="ACGI6B_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFG9751399.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            8866..10002
                     /locus_tag="ACGI6B_001956"
     CDS             8866..10002
                     /locus_tag="ACGI6B_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFG9751400.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            10061..10843
                     /locus_tag="ACGI6B_001957"
     CDS             10061..10843
                     /locus_tag="ACGI6B_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFG9751401.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            10862..11872
                     /locus_tag="ACGI6B_001958"
     CDS             10862..11872
                     /locus_tag="ACGI6B_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9751402.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            11891..12688
                     /locus_tag="ACGI6B_001959"
     CDS             11891..12688
                     /locus_tag="ACGI6B_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFG9751403.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            12786..13631
                     /locus_tag="ACGI6B_001960"
     CDS             12786..13631
                     /locus_tag="ACGI6B_001960"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HFG9751404.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            13718..14893
                     /locus_tag="ACGI6B_001961"
     CDS             13718..14893
                     /locus_tag="ACGI6B_001961"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFG9751405.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            15088..15990
                     /locus_tag="ACGI6B_001962"
     CDS             15088..15990
                     /locus_tag="ACGI6B_001962"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9751406.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            16004..17008
                     /locus_tag="ACGI6B_001963"
     CDS             16004..17008
                     /locus_tag="ACGI6B_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFG9751407.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(17152..17379)
                     /gene="acpP"
                     /locus_tag="ACGI6B_001964"
     CDS             complement(17152..17379)
                     /gene="acpP"
                     /locus_tag="ACGI6B_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFG9751408.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            17551..17976
                     /locus_tag="ACGI6B_001965"
     CDS             17551..17976
                     /locus_tag="ACGI6B_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751409.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            18024..18230
                     /locus_tag="ACGI6B_001966"
     CDS             18024..18230
                     /locus_tag="ACGI6B_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751410.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            18412..18591
                     /locus_tag="ACGI6B_001967"
     CDS             18412..18591
                     /locus_tag="ACGI6B_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751411.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            18625..18825
                     /locus_tag="ACGI6B_001968"
     CDS             18625..18825
                     /locus_tag="ACGI6B_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751412.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            19072..20094
                     /gene="ccpA"
                     /locus_tag="ACGI6B_001969"
     CDS             19072..20094
                     /gene="ccpA"
                     /locus_tag="ACGI6B_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HFG9751413.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(20217..20615)
                     /locus_tag="ACGI6B_001970"
     CDS             complement(20217..20615)
                     /locus_tag="ACGI6B_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751414.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            20827..21738
                     /locus_tag="ACGI6B_001971"
     CDS             20827..21738
                     /locus_tag="ACGI6B_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751415.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            21853..22033
                     /gene="ssrS"
                     /locus_tag="ACGI6B_001972"
     ncRNA           21853..22033
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGI6B_001972"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            22216..23433
                     /gene="cdeC"
                     /locus_tag="ACGI6B_001973"
     CDS             22216..23433
                     /gene="cdeC"
                     /locus_tag="ACGI6B_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HFG9751416.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(23534..24856)
                     /locus_tag="ACGI6B_001974"
     CDS             complement(23534..24856)
                     /locus_tag="ACGI6B_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFG9751417.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(24907..25701)
                     /locus_tag="ACGI6B_001975"
     CDS             complement(24907..25701)
                     /locus_tag="ACGI6B_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFG9751418.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            25811..27256
                     /locus_tag="ACGI6B_001976"
     CDS             25811..27256
                     /locus_tag="ACGI6B_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9751419.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            27285..27494
                     /locus_tag="ACGI6B_001977"
     CDS             27285..27494
                     /locus_tag="ACGI6B_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFG9751420.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            27514..28908
                     /locus_tag="ACGI6B_001978"
     CDS             27514..28908
                     /locus_tag="ACGI6B_001978"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9751421.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            29148..29363
                     /locus_tag="ACGI6B_001979"
     CDS             29148..29363
                     /locus_tag="ACGI6B_001979"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9751422.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNTTFLYKIAK"
     gene            complement(29442..30608)
                     /locus_tag="ACGI6B_001980"
     CDS             complement(29442..30608)
                     /locus_tag="ACGI6B_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9751423.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCL
                     RHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETVDILEGILQ"
     gene            complement(30745..30951)
                     /locus_tag="ACGI6B_001981"
     CDS             complement(30745..30951)
                     /locus_tag="ACGI6B_001981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751424.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(31010..31231)
                     /locus_tag="ACGI6B_001982"
     CDS             complement(31010..31231)
                     /locus_tag="ACGI6B_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751425.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNEDKDLTISILI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            31515..31652
                     /locus_tag="ACGI6B_001983"
     CDS             31515..31652
                     /locus_tag="ACGI6B_001983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751426.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            complement(31689..32084)
                     /locus_tag="ACGI6B_001984"
     CDS             complement(31689..32084)
                     /locus_tag="ACGI6B_001984"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751427.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            32263..32526
                     /locus_tag="ACGI6B_001985"
     CDS             32263..32526
                     /locus_tag="ACGI6B_001985"
                     /inference="COORDINATES: protein motif:HMM:NF020251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9751428.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNNNEESNHNQAK"
     gene            32495..33283
                     /locus_tag="ACGI6B_001986"
     CDS             32495..33283
                     /locus_tag="ACGI6B_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFG9751429.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKSSKQLSMLS"
     gene            33338..33475
                     /locus_tag="ACGI6B_001987"
     CDS             33338..33475
                     /locus_tag="ACGI6B_001987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFG9751430.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSKEIGMPIASIVIIACWLYISKI
                     N"
BASE COUNT        13012 a         3784 c         5761 g        10968 t
ORIGIN      
        1 tttttataca aattaaaaaa caacactatc tttttttgta tatatacatt gttatttatt
       61 tttatttaca aagcgtttcc atacatagct ccattttttc taggtgctat aatagcactt
      121 atgattaatc ctatatcaca aaagttagaa aataagtttc gtataaataa aggtatctct
      181 actcttgttc taagttttct tgcagtggct atagtcagta cagttactac aataatagta
      241 ataaattcaa tgaaagaatt aatgggattt ttaaataata tatctgctaa tcctgaggat
      301 ataagtaata ctattatgta ttttttaaat aaaataaatg actttatgaa atcatttcaa
      361 gaaatagcca attttgatgt agaacaatta gtaaacaaat tcagtggtga agtaatgcaa
      421 attacaaaaa atcttcttac tagtatactt ggattggcta cttctattcc atatattata
      481 atatttataa ttaccttgtt tattgctacg tactttatag caaaagacct tgataagatt
      541 gaaaatagtt tttataatat gtttactgtt gatgttagaa agaaggtaaa aaacgtaaag
      601 aaagaggcag ggctttcact tgttggatat ataagggcct atacaatact tatggctata
      661 acattttttg caatctgggg aagttttgct ttatttggat tgaaatacgg tctaatagta
      721 gggtttgtag gagctttaat ggatttaatt ccattcctag gtataataac aatatatctt
      781 ccagtaattg tttattattt ccttataaaa aattatttta tagctataag tatgactgtg
      841 atattttttg ttttgtcttt gattagagaa atacttgaac ctaaactagt atcagtaaat
      901 gttggactaa atccattagc taccctagca gcgattttta ttggaatcca agtaaaagga
      961 ataattggag taattttttg tttgggtttg gtatgtatgc atgatatact taagaaggtg
     1021 gatatttttt aattataata attgatagat tttttaaaaa taaaatttta ttcacaggta
     1081 aataaaaaaa tattaatctt attaagaaag agggtatata cgtgaaacaa aaagtagtgg
     1141 aaagattttt aaaatatgta tcatttgata caacatctaa ttctcagtgt gagaactgtc
     1201 catcttcaga aggtcaaaga gttttagcta agtatatagt tgaagagcta aaaacaatgg
     1261 gtgttgatga tgtgagttta gatgaaaata gttatataat ggctacttta aaaggaaata
     1321 ctgatggtgt ggatactata ggattcatat cacatttaga tacaattgaa gatgttagtg
     1381 gaaaagatat taaaccaaga ataatagaaa attatgatgg aaaagacata gttttaaatg
     1441 aagcgttaaa tgttattacg tatgtaaagg attctccaga gttggaagaa tttaaaggtg
     1501 acgatttaat agtcactgat ggaacaactc tacttgggtc tgatgataaa gctggtatag
     1561 ctgagatagt gactgcaata gagtatctaa taaatcatcc agaaattaag catggagata
     1621 taaaaatagg atttacacca gatgaagaaa ttggtagagg tgctgattta tttgatgttg
     1681 aaaaatttgg agcaaaatat gcatatactt tagatggagg aatagttgga gaacttgaat
     1741 gtgaaaattt taatgctgca aatgcaacca ttactataca tggtagaaat gttcatccag
     1801 gttcagctaa aaataaaatg gtaaatgcta tacatatagc tgctgaaata tcagaaatgt
     1861 tcccagcaga tgaaagacca gaaacaacag aaggatatga aggattttgg catttaaatt
     1921 ctataggtgg aaatgtcgag aatgtaagta tggcttatat tattagagac cattgtaaag
     1981 aaaaatttga aaatagaaaa tctattatga tagagaatat agaaaaaata aataagaaat
     2041 acgataatag agtggaattg gatttaaaag actcttatta caatatgaaa gaaaagattg
     2101 agccagttat gtttatagtt gatatagcta aagaagctat ggaggaatta ggtataaaac
     2161 cacgtttagt tcctgtaaga ggtggtactg atggagcaag attatctttt aatggattac
     2221 cttgtccaaa tatatttact ggtggattaa acttccatgg gaaaaatgag tgtataccag
     2281 ttagttctat ggagaaagct actaaactaa ttgtaagaat agctgaaaaa tatgctgaaa
     2341 gagtttagtt aaaaagtatt taaaaattaa gcatttaaag ttataatagc tatatcattc
     2401 tttataagtc aagctgatta taagcttgtg ttgtacttaa aatggtatag ctatttttgt
     2461 aattattttt ataaaagata attattttta gtttagggta tcaataaaaa gtatgaatat
     2521 caataggtat taacatttaa aagggggaat tgtattgaaa attaaatcta aaaaaataat
     2581 agcatttgca ctttcacttg ggattatgtt gccaagttgt gtatatgcag acactattag
     2641 taaaaatcaa aatgcacagg tagatatgaa caattatctt caaggtagaa aatctccaaa
     2701 ggaggaaaaa cttattggtg aagatagatt tgatacagcg ataaaaatat ctcaaagtgg
     2761 ttggaataat ggttcagaaa gggtgtttct agttaactca aattcattgc cagatgcatt
     2821 ggcttcaact cctttggcaa gtaaattgga tgcaccaata ctgcttacaa ataaaaatag
     2881 tataccatat aatgtaacag aggaaataaa aaggcttaat ccagcagaag ttattttaat
     2941 tggaagtgaa ggagctattt catcaagtgt aaaagaatct cttgaagata tggaagtatt
     3001 agtcagtaga atcggtggag cagatagaga agaaacatct ttacttctta ctagacagct
     3061 agatgatact ggtgatttag gtgtctcaaa agtggcagtg gttaatgggt ataatggact
     3121 ggcagatgca acttcaatat caagcccagc tgcaagtgat aatacagcta taatatatac
     3181 aggtaaggat agcataagaa gtgaggcaaa gaattttatt acacaaaact caactaagac
     3241 atacatagta ggtggggagt acagtataag taaaaaactt gaaggacagt tagtaaactc
     3301 agaaagacta gcagggacag atagaaaaga tactaatgcg aaagttttag aaaagtttta
     3361 tggaaaatca agtaaggtta ataatatgta ttttgcaaaa gatggaagtg gtagagaagc
     3421 tgatttagtt gatgggcttg ctgctggagt attagcatca aaaacaaagt cacctgtagt
     3481 acttgcaagt ggaagtttaa gtagtgcaca gaaaagtttt ataaaaaaag taaaggctga
     3541 taaatttgta caagttggtg gaggtaaaaa tagtaaacca tatgcagaag cattagcctt
     3601 acagtaatat tgtgtaaatc aaaattaata tataataata gcaagtaaac caagttgcac
     3661 ttcaatatat ataataaaat aagtaaacta agctatgttt caatataata tataataaaa
     3721 taagtaaact aagttatatt tcagtataat atataatgaa ataagtaaac taagttatat
     3781 ttcaatatag catataataa aacaagtaaa ccagattcta tttaattaga agtttaaggt
     3841 attgtactta aacttctaat tgagatagaa ataaaatggt ttacttgttt ttattatgag
     3901 tttaataaat taaagcttat tttattttta ttaaaaattt actaatcatt gatatttatt
     3961 atatttccag tattgatgtc aattaagttt atatcaagtt caagctcatc ctcatcatca
     4021 gtttttatga tatctataat tgcgacagaa tggcttccat cttttgggat agaagtactt
     4081 cctggattta ttactattaa attatcatcc atgtatagct tttttacatg agtatgtcct
     4141 aaaactaata tgtcaccacc cattttcttt gctttgtcaa taatttcttc ttctgattct
     4201 atataaccat gattcaatat aattctaatt tcaccaaatt ggctcatgac ataaggactc
     4261 tgaataggat gttctataac catctggtca acatctgcat cacagttccc ttttacaatc
     4321 acaatgtttt ctaatttatt taatgtctca ataaattttt taggattata tccttctgga
     4381 atatcatttc taggtccatg atataagaca tctccaccat gtaataaaac atcacaatca
     4441 gataatacat ttaacgcttt ttcaaagtaa agtaagctac catgagtatc actcattatt
     4501 cctattttca tattaaaaat cctcctaagt acagtctata aaaatttttc agatactcta
     4561 tgccaaaact catagtttga cattctaagt aattttattt ttgcatcaga tatactaact
     4621 tttatatttt cgatttgtct aaatctgtgc tcaactccat caacgacaat caatacagaa
     4681 tcttcaaatc tatattctgg tgcaatcttt ataacagatt catttgaaca tattatactt
     4741 gatgtaaagc acctatatgc attggtattt atcggatgta aaggtgtaat ctgcataagt
     4801 tcaagattta tatcaacaat actacctcca gcagaatagt tataggctgt tgaacctgtg
     4861 gaagtagaaa taatcatacc atctccacta aaattttgta tatgtttatt atcaaggctt
     4921 aaatttaggt gtatagtcct agacttgtct cctcttataa caacctcatt tacagcaagc
     4981 atattagaac cactagtagt tgtatatact tccgcattaa gaagtgacat ttcttgaata
     5041 atataatctt tttttgtata agcttcaata aagctatcaa tcttatctgg taaaatatca
     5101 ggaaaaaacc ccagatgacc tgtatttatt cccattatgg gaatttcagg aaaatcgaaa
     5161 tctctaactg ttcttaaaaa agagccatca ccaccaattg atataataag ctctgtatct
     5221 ggataaatat ctgaatacac ttcaaagcca gcattgataa gttttctagt taagagttct
     5281 ttagtctcaa gggacttatt taattggttt gtatttatag ttataattct tttcatatat
     5341 tcacctcttc atactttgta ttataacata gttttataat tcttaattta taaatttata
     5401 aatgaccttt gtagtgaatg tgttaaaata tgtataggtg ggtataaaaa aatgcataag
     5461 aggtgaagat gtgtttagca taaggaatat cacaaaaaaa ttaggaaaat tcaagttaaa
     5521 taatataaac ttagagttaa aggaagggga tattgttggg attataggac caaatggttc
     5581 gggaaaaacg actttaataa aaattataat ggggattatt gatgcagatg aaggtgaaat
     5641 tgaactttgt aatgaaacta ttgaaaattc tccaataagc tttaaaaaca atattggttt
     5701 tgtatatgat tctttacaat tttatccgca tttaaaggtt aaggaattta gaaaaatagt
     5761 ttctttattt tataagaatt ttgatagaga aaggtttgat gaatacctta ataagtttga
     5821 tatagaagaa aatatgcata ttgaaaattt atctaaaggt caatctgaaa agcttatgtt
     5881 atcaagtgca cttagtcaca atgctaagtt attgattttg gatgaaccaa ctgctgggat
     5941 agacccaatt gtaagaactg aaataatgca gtacttacaa gattttgtga aaaatggaag
     6001 tagtagtgta ataatttcta ctcataatac tgataattta attaaaattg ctgattatct
     6061 tgtttttata aatagaggaa atcagatttt tactgttaag aaagaattaa tagaacaaga
     6121 atataagata attagggcaa ataaagcaga gttagaagca attaaagaaa gcatagttgg
     6181 tgtgaaagag tataaatatt ataatgaggc acttgttaaa gttggagata gcttgaaagt
     6241 aaaaagccta ttgatagaaa ttgataagca taaggtcaaa aatcctacta tagaagaact
     6301 aatgtattat tatgtaaatg aggtgagatg atgttcaatt taataagaaa agatttaata
     6361 atagggatta gttcagatgg tatccgtaat ttaaagtaca tattgctatt ttttgtgttt
     6421 tattttttcc ttaattcaat ttcatattat acagtaagta ttgttgttag ttatttgata
     6481 tttataaata catttgagtg tgattatgaa aatgattcaa ggatttttat aaggagtatg
     6541 ccagttagta tagaagatat agtttactca aaatatttat taggtgtagg attaataatt
     6601 tctgtaacaa taattgtgtc cttactaagt aaattgacat cactagtgtt ctttagaaat
     6661 atggttttaa atgatgtatt tttttctgta aatatatttt tagcaatttt gacgatttta
     6721 ctgcctttaa tttttaagtt tggatatgga aaaatgaaag tttgtggttt tatagtaagt
     6781 attttaattt actttgtata tggaagtctt ttgaagatga ttagtatgat tgtatatcaa
     6841 gtaaagcatt ttaattatag taaagttgga ggagtatatc tttcaaatta tgtaactgat
     6901 atagctaata ctaagtatat aaatttatat agtatgacat ttttaacaat aataattttt
     6961 ataatatcaa tgtatttttc tattaagatt tcaaaaaaga ataagtttaa ttattaaaat
     7021 tttgattaat attatagcac aatagcacaa aatataagga gactaaaaat gaagaagttt
     7081 ataaaagttt cattttttat tttggggatt tttacactat tcatagctat aaataaatta
     7141 gtatatagag atatggtaag tgtcaaagat tatgaaataa atcatttaga ggaaattgtt
     7201 tttgattttg atagcaggag aagtagagat gttggtacta ttgtaagatt taaaataagc
     7261 aataatagta aatatagtta taaattaaac agtgctaaaa tgaagtttga aaattgtgtg
     7321 gaaaataaaa agggaatgaa taagtgttct gcatatgtaa atgtagattt atttaatgaa
     7381 aaaaattcta taactgaaag aactctgaaa tatggaataa aaccaaataa tcaaggttat
     7441 gttgattttg taattccaaa aggactaaat tttgattata ggtactttaa tgaatctggt
     7501 atgagtgtag agtataaagg agaatatatt gtcaatattc caatggcaaa aggactttat
     7561 ctagtagttg gaaaaaataa tgatacatgg aatgctaata ttatggaata atttgaaaaa
     7621 taaatcaatt atagatataa ttaagaataa tatgaaattt ataaaagttc agactaggag
     7681 gggtatttaa tgtcaataag taatgtaaga gagtatttta aacaatttgg caaggaagac
     7741 agtatattag aatttgaaca atctagtgct actgtggaac ttgcagctga agcagctggg
     7801 gttattccag caagaatagc taagacactc tcatttaaaa taggagatga tgcaatttta
     7861 attgtgacag ctggtgatgc taagattgat aataagaagt ataaggctga atttaattgt
     7921 aaggctaaaa tgttgacacc agaagaagtc ttggagttta ctggacatgc tattggtgga
     7981 gtctgtccat ttggtttaaa aaattctata aaagtctatt tagatgattc aatgaaaaga
     8041 tttgatacag tatttcctgc atgtggcagt agtaactctg caatagaact tacatgtgaa
     8101 gaaatggaga agttttctaa gtgtgaaaaa tgggtagatg tttgtaagaa ttggtaaaaa
     8161 aatatagtaa actatagtag gaattagtaa aaaactacct ctgtatacaa tctatacaga
     8221 ggtagttttt ttgtgtaaat atttataaat ctaagaggaa aatctaattt aaactaataa
     8281 tccaaaataa agttaaaaaa ttctgatttt ttttcgaaac aataaaaaaa tgacaaaaaa
     8341 gtggaaaaaa tgtaataaat attgttaaaa aaataattag aaattattga aaaattatcc
     8401 tttgagtgat actattatag atgtaagata tttaaaaata aaaaatagtc agataaaaat
     8461 atgattattt agctctatta aaataatttt tatacgataa aaatcttctg tacatggcta
     8521 aaaaacagat gtacagacaa tatttaatgt ttaatatacc ttaatataaa tatcgtaaac
     8581 aaattatttt atctctaacc agtgtgaaag aaatcttatt ctaacttgaa atttcacaaa
     8641 attccaaaaa gcgagatttt ttttaagtat agttgatttt aaattaacaa tttatgatta
     8701 aattaatgtc ataattggtt agtataatca catataaata tagagcgtag tttgattaag
     8761 gttataaatt tctcgacgta tattcaaata ccttattata gatgttgccg agattttaat
     8821 taataaatga aaataaaata tttataaaat actgaggagg ctattatgga tttaaattct
     8881 aaaaaatatc agatgcttaa agagctatat gtaagcttcg ctgaaaatga agttaaacct
     8941 ttagcaacag aacttgatga agaagaaaga tttccttatg aaacagtgga aaaaatggca
     9001 aaagcaggaa tgatgggtat accatatcca aaagaatatg gtggagaagg tggagacact
     9061 gtaggatata taatggcagt tgaagaattg tctagagttt gtggtactac aggagttata
     9121 ttatcagctc atacatctct tggctcatgg cctatatatc aatatggtaa tgaagaacaa
     9181 aaacaaaaat tcttaagacc attagcaagt ggagaaaaat taggagcatt tggtcttact
     9241 gagcctaatg ctggtacaga tgcgtctggc caacaaacaa ctgctgtttt agacggggat
     9301 gaatacatac ttaatggctc aaaaatattt ataacaaatg caatagctgg tgacatatat
     9361 gtagtaatgg caatgactga taaatctaag gggaacaaag gaatatcagc atttatagtt
     9421 gaaaaaggaa ctcctgggtt tagctttgga gttaaagaaa agaaaatggg tataagaggt
     9481 tcagctacga gtgaattaat atttgaggat tgcagaatac ctaaagaaaa tttacttgga
     9541 aaagaaggtc aaggatttaa gatagcaatg tctactcttg atggtggtag aattggtata
     9601 gctgcacaag ctttaggttt agcacaaggt gctcttgatg aaactgttaa atatgtaaaa
     9661 gaaagagtac aatttggtag accattatca aaattccaaa atacacaatt ccaattagct
     9721 gatatggaag ttaaggtaca agcggctaga caccttgtat atcaagcagc tataaataaa
     9781 gacttaggaa aaccttatgg agtagaagca gcaatggcaa aattatttgc agctgaaaca
     9841 gctatggaag ttactacaaa agctgtacaa cttcatggag gatatggata cactcgtgac
     9901 tatccagtag aaagaatgat gagagatgct aagataactg aaatatatga aggaactagt
     9961 gaagttcaaa gaatggttat ttcaggaaaa ttattaaaat agataaatat taaaatatac
    10021 acaataggtc gatattaaaa tagatatgga ggaaggattt atgaatatag tcgtttgtat
    10081 aaaacaagtt ccagatacaa cagaagttaa actagatcct aatacaggta ctttaattag
    10141 agatggagta ccaagtataa taaaccctga tgataaagca ggtttagaag aagctataaa
    10201 attaaaagaa gaaatgggtg ctcatgtaac tgttataaca atgggacctc ctcaagcaga
    10261 tatggcttta aaagaagctt tagcaatggg tgcagataga ggtatattat taacagatag
    10321 agcatttgca ggtgctgata cttgggcaac ttcatcagca ttagcaggag cattaaaaaa
    10381 tatagatttt gatattataa tagctggaag acaggcgata gatggagata ctgcacaagt
    10441 tggacctcaa atagctgaac atttaaatct tccatcaata acatatgctg aagaaataaa
    10501 aactgaaggt gaatatgtat tagtaaaaag acaatttgaa gattgttgcc atgacttaaa
    10561 agttaaaatg ccatgcctta taacaactct taaagatatg aacacaccaa gatacatgaa
    10621 agttggaaga atatatgatg ctttcgaaaa tgatgtagta gaaacatgga ctgtaaaaga
    10681 tatagaagtt gacccttcta atttaggtct taaaggttct ccaactagtg tatttaaatc
    10741 atttacaaaa tcagttaaac cagctggtac aatatacaat gaagatgcga aaacatcagc
    10801 tggaattatc atagataaat taaaagagaa gtatatcata taatttaagg ggggataaag
    10861 aatgggtaac gttttagtag taatagaaca aagagaaaat gtaattcaaa ctgtttcttt
    10921 agaattacta ggaaaggcta cagaaatagc aaaagattat gatacaaaag tttctgcatt
    10981 acttttaggt agtaaggtag aaggtttaat agatacatta gcacactatg gtgcagatga
    11041 ggtaatagta gtagatgatg aagctttagc agtgtataca actgaaccat atacaaaagc
    11101 agcttatgaa gcaataaaag cagctgaccc tatagttgta ttatttggtg caacttcaat
    11161 aggtagagat ttagcaccta gagtttctgc tagaatacat acaggtctta ctgctgactg
    11221 tacaggtctt gcagtagctg aagatacaaa attattatta atgacaagac ctgcctttgg
    11281 tggaaatata atggcaacaa tagtttgtaa agatttcaga cctcaaatgt ctacagttag
    11341 accaggggtt atgaagaaaa atgaacctga tgaaactaaa gaagctgtaa ttaaccgttt
    11401 caaggtagaa tttaatgatg ctgataaatt agttcaagtt gtacaagtaa taaaagaagc
    11461 taaaaaacaa gttaaaatag aagatgctaa gatattagtt tctgctggac gtggaatggg
    11521 tggaaaagaa aacttagaca tactttatga attagctgaa attataggtg gagaagtttc
    11581 tggttctcgt gccactatag atgcaggttg gttagataaa gcaagacaag ttggtcaaac
    11641 tggtaaaact gtaagaccag acctttatat agcatgtggt atatctggag caatacaaca
    11701 tatagctggt atggaagatg ctgagtttat agttgctata aataaaaatc cagaagctcc
    11761 aatatttaaa tatgctgatg ttggtatagt tggagatgtt cataaagtgc ttccagaact
    11821 tatcagtcag ttaagtgttg caaaagaaaa aggtgaagtt ttagctaact aatttcttgg
    11881 ggagggtatt atgagtacaa gtgatgttaa agtttatgag aatgtagctg ttgaagtaga
    11941 tggaaatata tgtacagtga aaatgaatag acctaaagcc cttaatgcaa taaattcaaa
    12001 gactttagaa gaactttatg aagtatttgt agatattaat aatgatgaaa ctattgatgt
    12061 tgtaatattg acaggggaag gaaaggcatt tgtagctgga gcagatattg catacatgaa
    12121 agatttagat gctgtagctg ctaaagattt tagtatctta ggagcaaaag cttttggaga
    12181 aatagaaaat agtaaaaaag tagtgatagc tgctgtaaac ggatttgctt taggtggagg
    12241 atgtgaactt gcaatggcat gtgatataag aattgcatct gctaaagcta aatttggtca
    12301 gccagaagta actcttggaa taactccagg atatggagga actcaaagac ttacaagatt
    12361 ggttggaatg gcaaaagcaa aagaattaat ctttacaggt caagttataa aagctgatga
    12421 agctgaaaaa atagggctag taaatagagt cgttgagcca gacattttaa tagaagaagt
    12481 tgagaaatta gctaagataa tagctaaaaa tgctcagctt gcagttagat actctaaaga
    12541 agcaatacaa cttggtgctc aaactgatat aaatactgga atagatatag aatctaattt
    12601 atttggtctt tgtttttcaa ctaaagacca aaaagaagga atgtcagctt tcgttgaaaa
    12661 gagagaagct aactttataa aagggtaaaa atctaattaa aaatatattt gctgttaact
    12721 agattaaata gtattttaat ctagttaaca gaatataaat actaaaataa caggaggtta
    12781 taactatgaa attagctgta ataggtagtg gaactatggg aagtggtatt gtacaaactt
    12841 ttgcaagttg tggacatgat gtatgtttaa agagtagaac tcaaggtgct atagataaat
    12901 gtttagcttt attagataaa aatttaacta agttagttac taagggaaaa atggatgaag
    12961 ctacaaaagc agaaatatta agtcatgtta gttcaactac taactatgaa gatttaaaag
    13021 atatggattt aataatagaa gcatctgtag aagacatgaa tataaagaaa gatgttttca
    13081 agttactaga tgaattatgt aaagaagata ctatcttggc aacaaatact tcatcattat
    13141 ctataacaga aatagcttct tctactaaga gaccagataa agttatagga atgcatttct
    13201 ttaatccagt tcctatgatg aaattagttg aagttataag tggtcagtta acatcaaaag
    13261 ttacttttga tacagtattt gaattatcta agagtatcaa taaagtacca gtagatgtat
    13321 ctgaatctcc tggatttgta gtaaatagaa tacttatacc tatgataaat gaagctgttg
    13381 gtatatatgc agatggtgtt gcaagtaaag aagaaataga tgaagctatg aaattaggag
    13441 caaaccatcc aatgggacca ctagcattag gtgatttaat cggattagat gttgttttag
    13501 ctataatgaa cgttttatat actgaatttg gagatactaa atatagacct catccacttt
    13561 tagctaaaat ggttagagct aatcaattag gaagaaaaac taagatagga ttctatgatt
    13621 ataataaata agaatttggg aattaaaagt ttaaataaaa ttgtttaaaa aacaatttcg
    13681 gatatatgaa aaatctaatt taatgggggt aatgaatatg agagaagtag taattgccag
    13741 tgcagctaga acagcagtag gaagttttgg aggagcattt aaatcagttt cagcggtaga
    13801 gttaggggta acagcagcta aagaagctat aaaaagagct aacataactc cagatatgat
    13861 agatgaatct cttttagggg gagtacttac agcaggtctt ggacaaaata tagcaagaca
    13921 aatagcatta ggagcaggaa taccagtaga aaaaccagct atgactataa atatagtttg
    13981 tggttctgga ttaagatctg tttcaatggc atctcaactt atagcattag gtgatgctga
    14041 tataatgtta gttggtggag ctgaaaacat gagtatgtct ccttatttag taccaagtgc
    14101 gagatatggt gcaagaatgg gtgatgctgc ttttgttgat tcaatgataa aagatggatt
    14161 atcagacata tttaataact atcacatggg tattactgct gaaaacatag cagagcaatg
    14221 gaatataact agagaagaac aagatgaatt agctcttgca agtcaaaata aagctgaaaa
    14281 agctcaagct gaaggaaaat ttgatgaaga aatagttcct gttgttataa aaggaagaaa
    14341 aggtgacact gtagtagata aagatgaata tattaagcct ggcactacaa tggagaaact
    14401 tgctaagtta agacctgcat ttaaaaaaga tggaacagtt actgctggta atgcatcagg
    14461 aataaatgat ggtgctgcta tgctagtagt aatggctaaa gaaaaagctg aagaactagg
    14521 aatagagcct cttgcaacta tagtttctta tggaacagct ggtgttgacc ctaaaataat
    14581 gggatatgga ccagttccag caactaaaaa agctttagaa gctgctaata tgactattga
    14641 agatatagat ttagttgaag ctaatgaggc atttgctgcc caatctgtag ctgtaataag
    14701 agacttaaat atagatatga ataaagttaa tgttaatggt ggagcaatag ctataggaca
    14761 tccaatagga tgctcaggag caagaatact tactacactt ttatatgaaa tgaagagaag
    14821 agatgctaaa actggtcttg ctacactttg tataggtgga ggaatgggaa ctactttaat
    14881 agttaagaga tagttttaga ttataatatt tacaaattaa atatttaatt atgaatttaa
    14941 cctgtaagaa taactatttg ttattcttac aggtttttat agttaaaaat aattattatc
    15001 tattaaacaa aaatagtgta aaatatataa tatcatgatt ttgaagatat aaaaagtagg
    15061 aagatataaa agaaagtagg taaaagtttg tttaaaaaag agaatcaaag atataatctt
    15121 atatcgtata caaatgaaga agaaatgact ttaaaagaag ttttattgga caagctaaat
    15181 ttctcagtta ggtcattatc aaaaatgaag agggaaaaga gtgttttagt aaatggagta
    15241 tataaaaaac cgagtttaaa ggtgtatagt ggtgatttaa tagaagtaaa aatagacgag
    15301 gaaaaagcaa attttgaacc tcaagattta aatttacaga taatttatga tgattttgat
    15361 ataattatgg tgaataaacc tccgtttatg gtagttcacc caacaaaaag ccactatgat
    15421 aaaacaatag caaatggtat tagttactat atagataatc aaaaagaaaa cgtaaaaata
    15481 agatttgtaa ataggttaga tatgaatact tcagggttag ttatagtagc aaaaaatgct
    15541 tatgcacatc atactttgtc tacagcaatg agtgaaaata aagttgaaaa aaagtatata
    15601 acagtagttg atggaattat aaaagaaaat gagggaacta tagatgagcc aatttataga
    15661 cctacagaag attcaataaa gaggataata gatgaaagag gacagtcatc tgttactcac
    15721 tataaagtaa tagaaagact agaaaatgca actgtattag aagtaagctt agaaactggt
    15781 agaacacatc aaataagagt acatatggct catataggtc atggaataat aggtgatgag
    15841 ttgtatggtt atgtagatga agagctaata aataggcaag cacttcatgc atataagcta
    15901 gagtttgaac aaccaagaac aaaagaaaaa ttgaaattta aagcagatat accagaggat
    15961 atgaaagagt taatatcgaa attaagataa tggggtgata tatatgataa tacataaatt
    16021 tataatacat gttttggaca aaaatagtga tgttccaata ttaaatgact ttgaaggcaa
    16081 agttaatcaa gaagtggatg gttttttcca gaaagctata aaaagaattg caaaagatga
    16141 ggatttaaga aaaggtgttt ttaaagatta taatgacaat ttaattaaaa actgttgtga
    16201 acaaataata tatgatgaaa gtactttttt gaaaaattct aaagaaattg catcttactt
    16261 gtttgacgtt atgaaaatta atgctgcact agaatcttgt gatttggcaa tttgtttata
    16321 cactattaaa gatgaaaaga gtgtagcaat actgaaatta gattataaaa agttgtacac
    16381 tcattcaatt gaatttgtgg atgataaatt taatattcaa atggtttcaa atgaaattgg
    16441 tatcccagag actttaagac aaaagcaagg agctttgatt agtctaagtg gtataaatga
    16501 tgagtttcat ctaaggcttt tagataagga tgctgaaaaa gaagggtcag aatctaagtt
    16561 tgtaacagaa tttttaaatg ctaaaaaaat agatgatgat aagtacaaga ccaaggtatt
    16621 taaaaataca gctgaaaatt ggataaccaa tgctcttagt aatgatataa agcaagctga
    16681 agatgtaaga agtatattaa attatacact gaaggaaaaa catgaagttg atataaatga
    16741 ctttgttgat aattcaatta aagatgatga gttaaaggat agctttaaag agcatatgga
    16801 agaaaaaggt cttgacgaaa gttttagtat agataaaaaa tgggttgaga aaaagcttaa
    16861 aaaaagaagt ataaaaacgg ataatggttt tgacataaaa gggaatctaa ctgattttga
    16921 agacccaatg aagtatactg taaaacaaaa tcaagatgga actatagata taataataaa
    16981 gaatgttacg ttttatgaag aaaagtaatt tatatactta gtatataaat taaatataag
    17041 agcgtgtatt ttatatattg taaataaatg taatctagtc tgtgcgaact ctaatataat
    17101 ttattatgta gaagtttaca cagactattt atatttgtag acaaaacgat actatttgtt
    17161 ttcttcgata tatttacata tatcaccaat acatttaaag ttctcagctt cctcgtctgg
    17221 tatttctatt ccaaattcat cttcaagggc cattataatt tcaacagcat ctaatgaatc
    17281 agcttctaag tcgtccatta aagatgcttc catagttatc tcatctaaat tgtctacccc
    17341 taattgttct gctattattt cttttatttt ctcaaacatt ttaagcctcc taaagcagtt
    17401 ttaattaatt ttaatgttag tttaatataa tatcaagaat ttttcaatat ggaatctata
    17461 aaaaataata aaattttata aatgtagtaa taaagtatta tttgatgaat aaactttatt
    17521 gataacaaat ataacttagg aggaaatgct atgaaatatg taggactatt actaacttca
    17581 gtaggaatgt ttttacttat agcagtaaat ttttattata attcaataac tttagatatg
    17641 caacgtatag aggattatgt aatggagacc aatttaatcc ttgaagatgt agctgaaaag
    17701 gagagttatg tatctaatga aaaagaagac tacatatcaa ggttaatgca tgtaaagaaa
    17761 ggaatagaaa attcaaaaac atccttcttg atagaaagat ataaggagta caaaataaaa
    17821 tcaatagaaa gtttaatcta tactatatca gaggagaaaa aagattatct ggatgaagtt
    17881 gatagataca ataaacttgg tgaaaaagaa ataaataaac taatcaataa aaacttttta
    17941 gaagtaactt atctgtctat tactacatat atttaattaa agagacaaaa cttagtctct
    18001 taatgattca aggaggatta gttatgacaa aattagaaga caatatggaa cgtttctttg
    18061 gaggatgcga aggatttttt gggaataaaa tgttgataat aatagttata gtattcttac
    18121 tattatgcac agacatttta gaagacttat tatgcgatga caatatatgg atatgggtta
    18181 tattaatagt attgttatta tttaacttcg atgatggttg ttgttgctaa aaaatattga
    18241 ttattaaata ggtaaaacca gtaacttata caagttatgg ttttaccttt tttagttcaa
    18301 attaaaaaat ttaaaaaaat agtaaaaata cattagcaaa atggaaacca aatgcatagt
    18361 atatgttagg agcgaaaagc tcaacaaaat atcaacagga ggtaacgtat aatgttagat
    18421 agattctttg gagaagaagg atgttttggt ggaggaggat ggtggataat agtactattc
    18481 ttcctattct tagcttttgg agatagctgg gcagaatttg acataatggc atggatacca
    18541 ttcctaatag ttttattaat actttgctgc tgtggtggat gcttagaata agaacacagt
    18601 tattaaaaaa aggagggctt tgttatgtta gatagatttt ttggagaaga aggatgcttt
    18661 ggtggaggag gatggtggat aatagtacta ttcttcctat tcttagcttt tgaagattgc
    18721 tggagagaca ttgacataat gtcatggata cctttcttaa tattattgct aataacttgt
    18781 tcatgtggtg gattctttga tggtgaagta ggttgcggct gctaatactt aaatcattag
    18841 aataaaaaaa tgctggtatt ttataccagc atttttttat tttatttttt aatatgattt
    18901 aaattaagaa tgaatattat taaatttgtt gaatataggg tataatataa aatataatgt
    18961 atttaaatgt acttgctttt atggtaaaat aaaaacttat ataaatgtag ttaaaatagg
    19021 aaccttcaaa aatttggttc agttttagat aaatttagta aaataaggag gatgaagatg
    19081 aaaggcaata taacgataaa agatgttgct aaacaagcag gagtgtcaat atctactgta
    19141 tctagagtta taaatgattc aaaacctgta actgatgaag tcaaacaaaa agttttagag
    19201 gttataaaag agactggata tataccaaat ccacttgcta gaagcttagt aacaaagaag
    19261 agtcaattaa taggggtaat agttccagaa gtttcagatt cttttgttaa tgaggtgtta
    19321 aatgggatag aagaggttgc taaaatgtat gactatgata ttcttttagc gaatacatac
    19381 tctgataagg aacaagaact taagagtata aatctattga gagcaaaaca agtggaaggt
    19441 atagttatga tttcatggat agttgaacaa gaacatatca actatataca aaattgtgga
    19501 ataccagcga catatataag taaaactgct agaaattatg atatatatac agtaagtact
    19561 agcaacgaag aagctacttt tgatatgaca gagcatctta taaagaaagg tcatgaaaag
    19621 atagctttta taatgacgag taaagatgat actgttttag aaatggaaag acttgctggt
    19681 tatgagaaag cactttcaaa taacaatata gaattagaca agagtttgat taagtatggt
    19741 ggaactgatt atgagagtgg atacaatagt atgaaagaac tattagatga tggaataata
    19801 cctcatgcgg cttttgtaac aggtgatgag gctgccatag gtgctataaa tgctatatgt
    19861 gatgctggat ataaggttcc agaagacata tctgttgcag gatttaatga tgttaagata
    19921 gctagaatgt atagacctaa acttactaca gtatatcaac ctctatacga tatgggagca
    19981 gtagcaataa gaatggttat aaagttaata aataaggaat taattgagaa taagaaaata
    20041 gaattacctt atagaattgt tgatagagaa agtgttacag aaagaaaaaa ataaaactat
    20101 taaaatcaat caggtagtgc ctaaggcact acctgatttt ttatgtcaat aatatcaaat
    20161 ttaataatag attaactaaa aataattatc taattactta aatagtttat ttttatctat
    20221 tcatgagggt tagacttttt agttttctta aataaacttt tagatttttt ttgttgacta
    20281 cctggtgtat tttttggtgc aggtgaattt gacttacctt tagtaggttt agccttctta
    20341 gttttagctg gtttagtatc tctttggttt tctgcatctg agattttttc cattatatta
    20401 tccagttttt tgcgttgatt ttcatctaaa aatgctttga attttgatac tacttcatct
    20461 ctgccatcta ggttcttacc tatttttata agttcctcca taagttcatt ttctgatttt
    20521 cctttgtaac tatcagccat cttttctatt ttatccttgt caatattttt ttgtttagcc
    20581 attttttcta atgactctgt atctaatttt ttcataaaaa aacccctttc tataaatata
    20641 tattgaatta tattcatact atttatatat agaaataata ttgaagtggt gaattgtgag
    20701 tgatatataa tataaataaa aattcttata gataatatat atgcactgag ctttaaaaat
    20761 ggaacattaa aatatgaaaa acatacttta atatagggca attatataat ataaggggga
    20821 attgtcttga ataacttact atttttgatt ggagtgattg cattgagtaa tggtaatatc
    20881 tctatatttg atgataaaaa taaatcaaaa ggaaataata gagttagaaa aaacaaaaaa
    20941 aatgcaaatg ataaactttc acagtctaca catggcaatt taaggtttga tgcaaatgat
    21001 ataaaaagag gtttaaggct catggattta agtgaagaag atttagaaat gggaatggaa
    21061 ataataacaa gaactaaaaa atacatgtca agagatgaaa gaaaaatact aataaaaata
    21121 gaaagtgttt tagatttagt tagaggaata aaaaaattaa ataatataga tatggtagat
    21181 acagaagatg aaacggattt tttcagaaaa atggatagtg atgataaaaa gaatatgatg
    21241 ataaaggaaa ttatagatgt attcccagaa aaaagaaaaa attcagttga aaaagctata
    21301 ggaatgaaaa agaagataga tttatttgca gaattatttt taccagatga ctttggtgaa
    21361 ggtggattta gtttgagctc tttggcaaat ataaacaatc ttggtagtat gaataatctc
    21421 aaattgttag gaagtctttt aagaggtgat gatagtaata atgatgatga agacaataat
    21481 gatgagttaa attatgaaga cgaagaatta gattgtgaag acaaagaatt ggatgatgat
    21541 tatgatgagt taacagacga aggtgaaatg gaggataatg aagatgaatt agtagaagat
    21601 aagaacacag gctatgaaaa tatagaaaat gatgaagata tagtatatga tgaggaacta
    21661 tacaacatta atgagcaaga atccgaatac aattataatg aaaaaaatac taaaactaga
    21721 gctaagaaaa aacgataatt aactaaatac ataaagaaaa aaggaagaaa aagtaatgat
    21781 agaataagat tatctcaatt actatataaa accactttaa aaaattcaaa atttattata
    21841 taataataat atgaaatcct gaaacatacg ataaggcttt tttaaattca acctacaaat
    21901 gaaatacggg agaccgtgtc tggtaaatta taacatgcct agttagtttg gacgttaaag
    21961 gttactggca agtcacccac ctgggagtgc ctagggtgtg aatttactta gccaccggtg
    22021 tttcgggaca aaaaaagtgc tactatttca aatagtagta ctttttttat taatatgcat
    22081 ttttctacta aatacgccta attattgata aattcaaaaa gcaagtaaca attaagatta
    22141 taaaagcata gtattaatta ggcttcaaaa agccaaagac ttaataagaa tcgcttacag
    22201 ggagggcaat gaaaaatgca agattataaa aaaaataaaa gaagaatgat gaatcagcca
    22261 atgtctacaa tgaatgaaga agaagtgtat acagatgaaa taaattcaga agacatgaga
    22321 ggttttaaaa aatcacacca tcataatgga tgtaatactg ataataagtg tgagtgccat
    22381 gatgattgca atccatgcaa cccatgtaat ccatgtaaac ctaacccatg caatccatgc
    22441 aaacctaatc catgtgatga caattgtgga tgccatgaca attgtaaatg tgattgtgaa
    22501 ccatgtgaaa tggattcaga tgaatgtttt gaaaacaaat gtggaccaga atgctgtaat
    22561 cctatatctc caagaaactt ctctgtatca aatgcagtgc catttgcaat agaggctaat
    22621 agaatatttg atactatgca attccaaaca tttacagatg caacaggacc aaatggagag
    22681 ccattaactt ttgaaacaga agtagtagaa gtatttggtt cagttccaag tgcaggtcaa
    22741 gcaagtgtaa ctatagaaaa aatatgctta agtaatgatg gaatcgttat agacacagga
    22801 atgacaactt tagaagattt cgatttagac ccattaggag atatagtagg aagaaactgt
    22861 gaaacaactt ttgaatttgc agtttgtgga gaaagaaacg ctgagtgctg tagacaagga
    22921 aaaggcaaat cagtagctta taaacaaaga ggattaactg tagcagttcg taatttagta
    22981 ctagagctaa gaggtagatg tggatgtaca gagttcgttg cattagcttt cccagcagtt
    23041 agagcaggag gtggatgtaa gagaagagtt gattatgtag aatttacttt taacacactt
    23101 tcagcaccaa tatgcttgcc agctgacgga agagctgtta ctttaagaca agaatatcaa
    23161 actaacttaa ctgtagattg tataggaaaa tctatattaa aattagaatg caacgaatgt
    23221 tgtgaacctt tctatgaatt aattatacca aatgatatag atttagtact ttgcttacaa
    23281 gaaacagtta gcacattaat aagtgaacaa atagtagttt tagcatcacc aaatccaatc
    23341 caaccaagac ttgttgatac tttctctaaa gtatgtgatt tttcgcaatg tggacctaat
    23401 catggaagtg gaaagccaag ttgccacaga tagataggta agatatatag acagtttaaa
    23461 ttataaataa aaatcatcta aggaaggcta ccaaagtgta taatttacat tggtagcctt
    23521 cacgttttta taactagcgt atagatttta gtgatttaaa aagcactata gtatgtaata
    23581 gacaagtaaa gattgatact atcgtcattg tgtatatgta tgcataaata ttaagtcctg
    23641 gaattggaag aaatacatat aaagctatta gttgaattaa catgccagta atagtattta
    23701 tacttgaagc taattctttt cttattgaat gtaaaatgcc agatagagtt tgatttaggg
    23761 acataaatag tggaactaaa aacatagctt ttagatattc tcctgcaagt gtatttttaa
    23821 atactaatat acataaatct tttccaaaga aataaaaaaa tattgatgat agtatgccta
    23881 caaataaagt aagaagtatg gagtatttga tttttttaat tacactctta cgctttctta
    23941 gagccatttc ttgtgaaata gttggtatta aatttacaat aagcgctgaa ccaacagtaa
    24001 aaggtaaaaa tataaaaggc attaccatcc cactgacaat accatacata cttaaagatt
    24061 gttgataagt cattcctgat aatgctaatc ttgatggtat catcatagaa cttatagatt
    24121 gcaaaagtat gtttgacatt ctattacaag ttatgggaat agacattttt aatgtttcca
    24181 tagaagaatt ataaaaatct tttatgctta taatatacct attgtcaaat gatgaatctc
    24241 gccataagca aataagcata aatataatat tactcaactc accgattgat atacctaata
    24301 gagctatata acaattcata gatttattat ttatgtacat aactagtaaa aaaacaaata
    24361 atattttacc aatttgttca atgatttgtc caatagcagg gatttttaca ttctttatgc
    24421 catagtaata tcctcttagt acgtttgaaa gagttattat aactatagca ggacaaacag
    24481 ctagtataaa tagatttaat ttagcatctt ttaaaaattt aagtgataaa aaagaactat
    24541 tgaaagatgc aactatagat ataattagtg ctacgaaaaa tgatatatat aaagttgata
    24601 taaataaagc attagtattg tgtctatcgt ttaaagcttt tctctttgca actagacaac
    24661 taagtgcagt aggtatacct gttgttgtaa cagctaagca aatcatcaaa aagttaaaaa
    24721 tcatatgata gatacctaaa cctgtttcac ctagtgctct tgataagaat attttatata
    24781 aaaaaccaaa tattcttaca ataaaattgg atataaataa aataactgtt gatagtacta
    24841 aataaggtac tttcaaaaaa atcacctcct tatcttttaa atatattcac aaggaggtgt
    24901 aaaaatttat aattttataa attctggttt caatttatta aatttagata catatttaaa
    24961 tcccatattt tttagtaatg aatatatgta gtcaaactca catgcaactt gacttatatg
    25021 atgtgaatct gaacctgtgg ttatgatttc ccctcccaag tctttataca tttgaagtat
    25081 atacttagat ggggttaggt ctggaagatt atatctataa caagaggtat ttatctcgat
    25141 acctttgcca tcataaatag cttgtttaag agtttcttca attatatcag aaaatagtct
    25201 atcatctaaa atagtgtcat aaggtgcata ccttttaatc aaatctaaat gacctaagac
    25261 agagtaatct ttataattct taacaatatt atataaataa agatagtaat tttcataaac
    25321 ttcatgttga gttttatctt taaaataatt accaaggtat aagtccattg tatctatagc
    25381 atggatagaa catattataa agtccaatgg atattgatgt gcttcctttt tgtatgtatc
    25441 cattaattga gtttgaactc caaattcaat tccttttttt attgatattt tatctttata
    25501 tttgctttga agagtctcta aactcttaaa ataatcttcg tagacaattg aaaaagaatc
    25561 atcagcatat acatcatagt caacatggtc tgtgaaacaa atttcctcca aaccgagttc
    25621 aatagatttt tttaccattt cttccattgt agattttccg tctgtagaga aacttgagtg
    25681 catgtgatag tcgtaaaaca taaatatttc ccctttttat aaaaattttg gtattatata
    25741 tacatactaa ataatatcaa atatacataa aagcatcaat aaaatataaa tacaatgagc
    25801 ggtgataaat ttggaaaaat acagaataga tttaaatgag aaaaaatcaa ctaaatatat
    25861 acagatattt aatcatataa aagaattgat aataaataaa gagttagtag aacatgaaaa
    25921 attgcctcca ataagaaaac tatcaaattt tttacaagta aataacacta ctatagttaa
    25981 ggtatacgag cttttagaaa atgaaggata tgtatataaa attataggga gtggtacttt
    26041 tgtatcaagt ttaaattctt ataaaagcgg tatagataac aggaaaaata agattattgt
    26101 gcaaagagaa gatttaattc attttgacaa tgggaatcca tctaatgata tgtttcctat
    26161 cgattcattt aaaaatgctg taaatatggc attatctaaa gatggtagtt caatatttga
    26221 atatgatgaa ggtcttggct ctgaagaatt aagagaaaaa atggtagagt atctaagtga
    26281 tggaaatata aaaactacta aggaaaatat tcaaataata tcaggtgctc aacaaggtat
    26341 agatatagtt tgtaaaggac ttataagtta ttcagatgtt gtttttatgg aagaaccttc
    26401 ttacaatgga gcaattgaag tgtttaaaag tagaggtgca aaaataatat ctatacctat
    26461 gttagatgat ggaattgata taggtatttt aaaattaaag ttggaaaaaa taaaaccaag
    26521 acttatatac attatgccta attttcaaaa tccaactgga atatcttact caacatataa
    26581 aaaaaagaaa ttgatagaat tggcagaaga acatgatttt tatataatag aagatgattt
    26641 tataagtgat tttgtttttg aatcaaagga caatagaaca attagaagtt atgatgataa
    26701 aaatagagta gtatatataa aaagcttttc aaaaatactt atgccaggtc ttaggatagg
    26761 aattgtagaa atgccaaatg agttgttaaa aaaagtttta tgggctaaat attcatcaga
    26821 tatatcaaca ccaggtttaa ttcaaaaatc aatgtattac tatatgaata attttgattg
    26881 gaagaattat ttgagtcata tagaaaagat atatactgat aagtataaat tggcaaaaaa
    26941 cttgattaat gacaaattga gtggtaaatt aaaagttaga aaatcttgtg gaggaataaa
    27001 ctttttttta gaattaccaa gaggatatac atctcaagca tttacaaatt ttatgttaaa
    27061 caaaggagtg tcaatgcttc cagggactta tttttttgac aatctagtgg atgatagatt
    27121 ctttaggata aatatagctc gcccaagcat ggaagaacta gaaaagggaa tatctataat
    27181 aagtgataat atagatgagt ttttttgtga atataaaaac aagttagata taaaaagcaa
    27241 taaattattt tattaaataa atagaggttt tttaggagga aaaaatgttt gacaagaaga
    27301 aattagatag gattaatgaa ttagcaaaga aaaataaaga gggaatcctt tctgctgatg
    27361 aaatcaaaga aagagaaatt ttaaggaaag aatatttaga aaactttaga gcccacttta
    27421 gatcaagact agatagtgtc aaagtagttt caccagaaga atatgagcaa tacatgaaaa
    27481 acaataaaaa ttagaactta gtaggaggaa aatatgaact taaagtacga atttaagaat
    27541 gcttgggatt ttgaaagaaa agaaaatact attgaacaaa ttatgcaata ttctagcaat
    27601 tatatggagt ttttaagtaa gtcaaagact gaaagacttt cagtaaaaga aattataaaa
    27661 ttggctaaag aaaataacta tatttcaata gatgaagcta tggaaaaagg aagcataagt
    27721 tgtggtgata agatttatgt tataaataaa gagaaggcag tagcactttt tgtaataggt
    27781 aaaaattata tagaaaaagg tatgaagatt ataggaagcc atatagattc gccaagatta
    27841 gatttaaaac caaatccact gtatcaagag agtaatctag gtttttttaa gactcattat
    27901 tatggtggaa taaaaaaata ccaatggacg gctattccac ttgctttaca tggtatagtt
    27961 atattaaatg atggaacaaa agtggacata tctattgggg aagaagatag tgacccagta
    28021 ttttgtgtaa ctgatttatt gattcatttg gctggagacc aaatgcaaaa gaaactatca
    28081 gaaggaatta gtggagaagc tttaaatata ttaattggaa atatgccttt agaagatgaa
    28141 gaaaaagaac ctataacagc aaatatatta aagattttaa atgagaaata caatatagtt
    28201 gaagaagatt tattaagtgc tgaaattgaa gtagttccag ctggtaaggc tagagattta
    28261 gggcttgata ggtctatggt gttgggatat gggcatgatg atagggtatg ttcatatgct
    28321 gctgtaaaag ctatactaga aactgaacaa ccagaattta cttcagttgc attatgtgta
    28381 gataaagaag aaattggttc taaagggaat acaggtatgc actcaaagtt ttttgaaaat
    28441 actgtggctg aactgattgc tttagaagga gattattgtg atattaaagt aagaagagct
    28501 ttagcaaata gtaaggtatt atcagctgat gtttctgctg gatatgaccc aaattttgga
    28561 gaagcatatg aaaagagaaa ttcggcatat atgggaaatg gagtagtttt aactaagtat
    28621 acaggttcaa gaggaaagag tggatgtaat gatgctaatg ctgaatttat gtcagaagta
    28681 aagagaattt ttaataaagg aaatgtaata tggcaaactg ctgaacttgg aaaagtagac
    28741 caaggaggag gcggaactat agcccatata ttggcaaatc aaggtgcaga ggttatagac
    28801 tgtggtgttg gagttttaaa tatgcatgca ccacatgaga ttgtctctaa agtagacata
    28861 tatgaaatgt ataagggata taaggcattt tttaatataa atttataagt aaaaatataa
    28921 atgtaataag tgaataaagg ctgttgtata aacaatttaa tctgttgtac aacagtctta
    28981 tttttatgta aaaatagcaa tttgtcacac tagagaaaat aaaagaaaat actgtcaaat
    29041 taaaaattat ttaaaaaaaa attgacaacg atttcatctt gttataatat gtaataaaga
    29101 acttgttata taatgttata aaaatgttac atagggaggt gagcaaaatg gatgaaaaga
    29161 gacaaaaaaa agaaatggat ttagaagtaa attcctttgt tcctttatat caacaactat
    29221 atgacaacat aaaaaaacaa atagcatctg gcatatataa accaggagat aaacttccat
    29281 ctgaaggaga cttatgtaaa gaatttaata taagtcgtat aactgtgaga aatacgactt
    29341 ttttatataa aatagcaaaa taacagtgtt tgagcaaaaa aaagaatgtt ttttatcgtt
    29401 ttgccacctg tttgccacca taattttttt tcggtggcaa attattgtaa aataccctcc
    29461 aaaatatcta ctgtttcact ttccatttta cttgtaacat gtgaatatgt atccatagta
    29521 gttgataatt gactatgtcc taatctttgt tgtatatatt ttatattagc tccattttct
    29581 aataataatg tagcatgtgt atgtcttaaa caatggaagt tgaagtcaag gtttaatttt
    29641 ttgttaattg ttctcacaca agcatcaatg ttgttatgat ttacaaatga gccatctttt
    29701 tttctacaaa cccaatcata ctcagtttct ttataccatt ttccaatttt aattttttgt
    29761 tttttttgat tcaatttttc ctcttttaat attctagaca aagtatcacc tattttaata
    29821 tctcttattg atgtttttgt cttaggtgat gctaattcaa attctgaaac ttttctcttt
    29881 attaaatttt ttctaacttt gattatatta ttatctaaat caacattatc ccaacaaaga
    29941 cctaatattt ctccttttct catgcctgta tgaaacccta taagtagagg aatatagata
    30001 tttgtatttt taggatatat ttctagtatt ttattgaact catctagtgt tatagtcttg
    30061 ttatctgact cattttttac ctttaaaaca ttttttggta tactggcata ttgaacagga
    30121 ttttccttaa tgagtttgta agggtaaaca gcagatttta aagcagcatt taatacaaca
    30181 taaatcgctt ttaaaactcc ttttgtgtag tgtttttctt ctccgttttg tgtgtatgtc
    30241 tcttttgatt tattatttaa aaattcttga ataatagctg gatttataga ttttagctta
    30301 catttaccaa gtctaggttc tatatgattt tcaattaaat ttctataact ttcctgagta
    30361 ttgtatttgc aattaagaag tacatactct ttgtaccaaa agttaagata gtctgataaa
    30421 ctcatatttg tttcatcaaa cactatacca gcgttttcat attcatttat tgcatcacgg
    30481 agcgattttt cagcttcttt cttagtgttt ccaccaactc tttctacctt ttttctcttg
    30541 ccatctacta tacctgcatc aaagtagtaa taccacttct ttccacgttt tcttacgccg
    30601 cctttcataa aatttctcct ctcaaaagct tttttatgta attatataat aacatatatc
    30661 aatttaaata aaataaataa aaaagactat aagaaacaaa gttttatgtt tataattaat
    30721 agtctttttt atttagatac atatttaatg ttttttatca cgtgattcca gcactttttc
    30781 tgccaaataa acaactattt tttcatactc aatatcagtt tctaaagaaa agtcaacaat
    30841 agcaggtatc gtcatatctc ctgcatattt attacctaac caattttcaa ttaattccaa
    30901 ttcttctttg cttaattcaa attttttctt tttattaaaa cttaagttca atttattcac
    30961 ctcaaataat attatatatt aaaaatagta tttgatagaa acattcgaat tatttatgta
    31021 attttctttt tttaatatat tcatcagcaa aataatttac taaatatttt tcttccaatc
    31081 ctaaagctat ttctaattca ataagaatag agatagttaa atctttatcc tcattatttt
    31141 ccaacctaga aatttgactt ctgtggcaac caactctttt tgctaactct atttgtgtta
    31201 atttcttctt ttttcgtaac tcttttaaca tatatttaac ctacctttta gataatttta
    31261 tatatgttag tttgtgtaat ttcttgtaat aaatgtgcaa tattcgcaca ctttttgttg
    31321 gaaatttgtg ctagaatgta gttaagaaat aactttatct agataaagca aaaataatag
    31381 aagctataaa atataataat attttacagt tattttatag ttttaagctt tgaaaaagag
    31441 gtggttgtaa ctatttttaa gaacgtatgt ttcgtggaat taataaataa aatattttat
    31501 aggggatgat atatttgaat aagacgaaat attatgatat tttaaagtta aacttattaa
    31561 tgaaaaagtt gaaagaattg gataaaaata aaatcaatga atataaaata aaagtgaaag
    31621 aaatacataa aatcaataaa aaagaggaat gattcctctt ttttattata ttcattttca
    31681 gaaataaact attttttatt tttttcgtct agtaaaaata attctgcaac ttttaatgct
    31741 ttttctcttg cgtctgggct tagctcgcta aatatattaa aaacttcttt catatcttcg
    31801 tcaagataca tattctcaat aagctccttt tctgttttaa aattttcagc atcataatct
    31861 ttttcgtttt tatttataaa taagctattt ctaacatcag ttcttcctaa taaataatct
    31921 gtagacacat caaaatagtt tgcatattct tctattgtac ttttcttagg ttctcttaaa
    31981 ccattttcta tccttgatag tgtagatttg tttatatgta aatcctcgct taatttgtct
    32041 agcgagatgc ctttttcttc tctcaattct tttatcctat tcaatttgca caaccccttt
    32101 ttatattaat tccaaaatag caacttttat ttatattata gcaacaaaat taattattac
    32161 aatttttttt gctaaaaaag caacaaaagt attgactata atcacaaaac ttgatattat
    32221 ataaatatga agttgccaaa atagcaaaaa ggaagtgatt taatgtactt aaatagatta
    32281 gaaggattga tgaaggaaaa tagacacacc caaaaaaatg tggcagatat attaggtctt
    32341 agctcatatg gttttagatt aaaattaaaa ggaaaaaacg aatttaaagc aagtgaaata
    32401 aaaaagatat ctaaattata taatgtatct gcggattatt ttttttcaga tgaagttgct
    32461 aaaatagcaa taaaagaaga aaggggcaat aataatgaag aatctaacca taatcaagca
    32521 aaataatcaa tttttagttg aaagtagaga agtagcagaa ttaatagaaa agaagcacga
    32581 taatttatta agagatataa gaggatacaa gaagatttta gaggactcat caaatttgag
    32641 gagtcaagat ttctttatag aaagtactta tataaatact caaaataaaa tccaaccttg
    32701 ctacttatta actaaaaaag gttgcgatat ggtagctaat aaaatgacag gagaaaaagg
    32761 gattatattt acagcaattt atgtaactaa gtttgaagaa atggagcaag agttaaaaga
    32821 acaacaacct aaattaccaa ctacatataa agaagcactg caacagttat taatcgaagt
    32881 tgaagaaaaa gaacaactac aattagaaaa tcaagaaaag gataaggtaa tccagttaca
    32941 acaaccaaaa gtactgtttg ctgattcggt agcgtcttct gacaattcaa tcctagttgg
    33001 agaattagca aaattgctta gacaaaatgg aattgataca ggacaaaata gattatttga
    33061 ctggttaaga aataatggtt acttaataaa acgtaaaggt gaggattaca atacaccaac
    33121 tcaaaaaagt gtagatttag gagttataga aacaaaagaa ggtacaagag tacatccaga
    33181 tggtcataca agtattacta aaacacctaa gattactggt aagggacaaa tatacttcat
    33241 taataaattt aaaagtagca aacaattatc aatgttaagt taaaacataa cagtaccttg
    33301 aaaactaaat acagaatatt caaaagaggt gattgaattg aaacacgagc aaacaacgct
    33361 tcgtatacca gaagacttgt acaaagcgtt gatagattta agtaaggaaa tcggaatgcc
    33421 tattgcatct attgtaataa ttgcgtgttg gttgtatata tcaaagataa attaaccaat
    33481 gagatatgca aaatgaatgc acagcattat ttattagaga attga
//
LOCUS       DAYAOY010000041        33246 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYAOY010000041 DAYAOY010000000
VERSION     DAYAOY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.041"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            276..1439
                     /locus_tag="ACGI6B_001988"
     CDS             276..1439
                     /locus_tag="ACGI6B_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9751431.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            1488..2339
                     /locus_tag="ACGI6B_001989"
     CDS             1488..2339
                     /locus_tag="ACGI6B_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFG9751432.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            2665..4566
                     /locus_tag="ACGI6B_001990"
     CDS             2665..4566
                     /locus_tag="ACGI6B_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751433.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            4569..5612
                     /gene="ypdE"
                     /locus_tag="ACGI6B_001991"
     CDS             4569..5612
                     /gene="ypdE"
                     /locus_tag="ACGI6B_001991"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9751434.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            5627..5932
                     /locus_tag="ACGI6B_001992"
     CDS             5627..5932
                     /locus_tag="ACGI6B_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9751435.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            6029..6364
                     /locus_tag="ACGI6B_001993"
     CDS             6029..6364
                     /locus_tag="ACGI6B_001993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9751436.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            6396..7718
                     /locus_tag="ACGI6B_001994"
     CDS             6396..7718
                     /locus_tag="ACGI6B_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9751437.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            7728..8798
                     /locus_tag="ACGI6B_001995"
     CDS             7728..8798
                     /locus_tag="ACGI6B_001995"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9751438.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            8803..9990
                     /locus_tag="ACGI6B_001996"
     CDS             8803..9990
                     /locus_tag="ACGI6B_001996"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9751439.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(10187..11059)
                     /locus_tag="ACGI6B_001997"
     CDS             complement(10187..11059)
                     /locus_tag="ACGI6B_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFG9751440.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(11453..11644)
                     /locus_tag="ACGI6B_001998"
     CDS             complement(11453..11644)
                     /locus_tag="ACGI6B_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751441.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            11864..12910
                     /gene="cobT"
                     /locus_tag="ACGI6B_001999"
     CDS             11864..12910
                     /gene="cobT"
                     /locus_tag="ACGI6B_001999"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFG9751442.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            12942..13502
                     /gene="cobU"
                     /locus_tag="ACGI6B_002000"
     CDS             12942..13502
                     /gene="cobU"
                     /locus_tag="ACGI6B_002000"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFG9751443.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            13512..14285
                     /gene="cobS"
                     /locus_tag="ACGI6B_002001"
     CDS             13512..14285
                     /gene="cobS"
                     /locus_tag="ACGI6B_002001"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFG9751444.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            14305..14919
                     /locus_tag="ACGI6B_002002"
     CDS             14305..14919
                     /locus_tag="ACGI6B_002002"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFG9751445.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      14986..15165
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15280..16794
                     /locus_tag="ACGI6B_002003"
     CDS             15280..16794
                     /locus_tag="ACGI6B_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HFG9751446.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            16833..18209
                     /locus_tag="ACGI6B_002004"
     CDS             16833..18209
                     /locus_tag="ACGI6B_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HFG9751447.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            18210..19181
                     /gene="cbiB"
                     /locus_tag="ACGI6B_002005"
     CDS             18210..19181
                     /gene="cbiB"
                     /locus_tag="ACGI6B_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HFG9751448.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            19213..20283
                     /locus_tag="ACGI6B_002006"
     CDS             19213..20283
                     /locus_tag="ACGI6B_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9751449.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            20285..21172
                     /locus_tag="ACGI6B_002007"
     CDS             20285..21172
                     /locus_tag="ACGI6B_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFG9751450.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            21160..21792
                     /locus_tag="ACGI6B_002008"
     CDS             21160..21792
                     /locus_tag="ACGI6B_002008"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HFG9751451.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            21801..23027
                     /gene="cbiD"
                     /locus_tag="ACGI6B_002009"
     CDS             21801..23027
                     /gene="cbiD"
                     /locus_tag="ACGI6B_002009"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HFG9751452.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            23024..23632
                     /locus_tag="ACGI6B_002010"
     CDS             23024..23632
                     /locus_tag="ACGI6B_002010"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HFG9751453.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            23622..24197
                     /locus_tag="ACGI6B_002011"
     CDS             23622..24197
                     /locus_tag="ACGI6B_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HFG9751454.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            24190..24942
                     /locus_tag="ACGI6B_002012"
     CDS             24190..24942
                     /locus_tag="ACGI6B_002012"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HFG9751455.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            24926..26047
                     /gene="cbiG"
                     /locus_tag="ACGI6B_002013"
     CDS             24926..26047
                     /gene="cbiG"
                     /locus_tag="ACGI6B_002013"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HFG9751456.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            26044..26769
                     /gene="cobJ"
                     /locus_tag="ACGI6B_002014"
     CDS             26044..26769
                     /gene="cobJ"
                     /locus_tag="ACGI6B_002014"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HFG9751457.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            26766..27518
                     /gene="cobK"
                     /locus_tag="ACGI6B_002015"
     CDS             26766..27518
                     /gene="cobK"
                     /locus_tag="ACGI6B_002015"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HFG9751458.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            27537..29015
                     /locus_tag="ACGI6B_002016"
     CDS             27537..29015
                     /locus_tag="ACGI6B_002016"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HFG9751459.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            29045..29950
                     /gene="hemC"
                     /locus_tag="ACGI6B_002017"
     CDS             29045..29950
                     /gene="hemC"
                     /locus_tag="ACGI6B_002017"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFG9751460.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            29940..31439
                     /gene="cobA"
                     /locus_tag="ACGI6B_002018"
     CDS             29940..31439
                     /gene="cobA"
                     /locus_tag="ACGI6B_002018"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFG9751461.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            31456..32421
                     /gene="hemB"
                     /locus_tag="ACGI6B_002019"
     CDS             31456..32421
                     /gene="hemB"
                     /locus_tag="ACGI6B_002019"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFG9751462.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            32574..33152
                     /locus_tag="ACGI6B_002020"
     CDS             32574..33152
                     /locus_tag="ACGI6B_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HFG9751463.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        13466 a         3326 c         6125 g        10329 t
ORIGIN      
        1 aatttatatt tggattagtg gtaattacca ctttggtctg aacaagcttt tatcgtaaaa
       61 taaaaatact aaagtatata gagtatgatt ttattaaatt tttaaagata aactataaaa
      121 ttaattaaaa tctatagtgt atattttata gtgagtttaa gtagtactaa aaaatctaag
      181 caaaaaatta taaaagagta tattttttta tagtttaaag tggttataac ctcattacca
      241 aatgtaaacg ttacaagtat gaaaggagaa tgaaaatgtt taaattagaa gaacaaaaac
      301 taaaagattt aggtgcaata ataacaacta atgaaataaa acaacagcct gaattatggt
      361 tagaaactta tgaaatatat aaatctaaca aagaaaaatt aagtagattc atagatacaa
      421 taagtaataa tcatgggcag tttagagtta tatttacagg tgctggaact tcagcttaca
      481 taggaaattc aatacttcct taccttaaaa ataaaaatga cataagaaaa tacatatttg
      541 aagcaatacc tactacggat atagtttcaa acccatatga ttacttaaaa aaagatatac
      601 caacattatt aatttcattt gcaagaagtg gaaatagtcc tgaaagttta gcagctttaa
      661 atttagggaa taagattgta gataattttt atcatttagc tataacttgt aatccagaag
      721 gagaactggc taagatgact aaaaacgacg aaaataatta cttgttatta atgccatcaa
      781 aatcaaatga tgaaggcttt gccatgacag gaagtttttc gtgtatgatg ttatctgcta
      841 tgttaatatt cgatagttta gaagatgatg tagaaaagtc atatataaat gcaataattg
      901 aaatgggaag aaatgttata gatagaaaag atgaaataca tgagttaata aataaagatt
      961 ttgatagagt tgtatattta ggttcaggag gattaggtgg tttgacacaa gaagcacaac
     1021 taaaactttt agaacttaca gcaggaaaaa tatctacagt gtatgattct cctatggggt
     1081 ttagacacgg accaaaatca tttattgatg aaaatacact tgtatttgaa tttgtatcta
     1141 actgtttata tacaagaaaa tatgatttag atgttttaga ggagataaaa agggataaaa
     1201 ttgctaaatt cacttgtgca gtgtcagtag aaaatgaaaa taacttctca ggaacaaagt
     1261 ttgagtttaa agaaaagtat aataaattac cagatgtgta tcttgcaatg ccatacatat
     1321 tgtttgcaca aactatagca ttgtttgtat cagtaaaggt tggaaataag ccagatactc
     1381 catcagctac aggaacagta aatcgagtag taaaaggtgt aacaatatat gaatattaaa
     1441 agataagatt aaaatattat aactaaataa tttgggaggg tatcaatgtg gcattagtaa
     1501 caacaacatc aatgcttaaa aaggcacaag atgaagggta tgcagtagga gcatttaatg
     1561 tagaaaatat ggaaatggta attgctgtta tagaagctgc tgaggagtta aattctccag
     1621 taatattaca aactactcca tctactgtaa aatatgcagg tcttgattat tatctagcaa
     1681 atgcaaaggt tgctgcagaa agagtaaata tacctgtagc aatgcatctg gaccatggtt
     1741 caagttttga ccttgcaatg aaagctttaa gaagtggata tacatcaata atgatagatg
     1801 gttctcatga aagctttgaa aataatatat ctatatctaa atcagtagta aaagcatgta
     1861 gtccttctct tataccagtg gaagcagaac ttgggaaagt tggtggaaaa gaagatgatt
     1921 tagatggcgg gaatagtaat gattatacaa atccaaaaga ggcaaaagag tttgtcgata
     1981 gaacaggaat atcttcattg gctattgcta taggtactgc acatggattg tataagggtg
     2041 aacctaaaat agatttagat aggttatcag aaattaaaaa tgtagtttca atacctttag
     2101 tattgcatgg aggttcagga atacatgatg atattataaa agaagctata aaaagaggta
     2161 ttgcaaaagt taactatgca actgaattaa gaattgcata tagtaaaggg gttaaaaaag
     2221 ttttagatga agattctgag gttatagacc ctaaaaagta tggattagca gggcttcaat
     2281 cagtaaagga ttttgtgaaa gaaaagatta aagtgtgtgg gtctgtaaat agagtataaa
     2341 aataaaatat cctaaattta ttttagataa gtttaggata tttattatat gaaaaattta
     2401 tattaattat atgtaaatgg tgtatttttt tcatttaatt gatgaaaaat atcagctttt
     2461 attggtaaag tattacatat ataattaaaa tatactaaaa attgaactat atattttatt
     2521 attataaaat atttatatta aattgtggaa ttaaaatata ctaggatata aaattcttaa
     2581 ataaaaagaa aaggttgttt ttataaaaaa tattttttat accactttaa aaaacacaat
     2641 aataacactg agggaggtgg ggatatgaga gaacgcatta ttcttgaaac attaaaaaat
     2701 agtagtggtt atctaaacat agattatttt gcagataagt tgggggtatc tactcgaact
     2761 atcagaaatg aaataaaaaa gatagaaact ataaaagaat gtaatggttt taaattagaa
     2821 tataagtcta aattaggata tattctaaat attaaagacc aagataaatt cgagcattat
     2881 ctaagaaact taccctcaga tttagttgaa aatccagaac aacggttgga gtctataata
     2941 gtagagcttt tagtaaatga aggttataaa actatagaac agttaagtaa aaaatttcta
     3001 gtaagttcat cccaaataaa aaatgattta aaaaagatag atgaaaagat aaaggataca
     3061 gaattaaaac tagagcgaaa ggctcattat ggaattaaaa tagaaggttc tataaaagaa
     3121 attcaaagta tattggtgga tagttatttt agagggaata gaaatataac agagtacagg
     3181 aataagttta tagataattt aaaattagct aatattagaa gtacaataaa aaatgtactt
     3241 aatgaacatg atttagaagc aaatttaaca gaacttgagg aaatattagc acaaattatt
     3301 atattgtata taagagttag tatgagagta gtgggaaatg ctagcgattt aaaattatat
     3361 acagaagatt taattataga taaattatta gataaaattt ttagagataa aaaatataac
     3421 cttaattatg atgaaaagta ttatttaaag gagcttataa aactaaagac taaagataag
     3481 aaagccacta taaagaatat tgataagaat aaacttcaag atattatgtt cgaatttttt
     3541 agagagatag ataaaaaata taactctaat tttttagaag ataaagagtt ttttaactta
     3601 ttttacttac atattgcatg tttaatagaa cgtataaaga agaaccacaa gataataaat
     3661 cctttttcag ttaaaataag tcaacagtat ccaactgttt ttaatttatc tattcaattt
     3721 tcaaaaatca tagaaaatga gtatcatata aaaataagtc aagatgaaat agggtttata
     3781 gctactcaca ttgctgttcc ttttgaaaag agagaagaag ctaattttaa taaaaaatat
     3841 aaaatagcaa taatatgctc ttctggtgga ggtagtgcat ttttgataaa tttaagactt
     3901 aaagaaattt ttccaaatgc tgagataaga aatttttcat tactagaaga aaaagctgta
     3961 atagaatttg caccagattt gattttttca ataacaaatc ttttatttga aataaatgct
     4021 ccagttatac ttataaatga aattttagac gaattggatt atttagatat taaagaaagt
     4081 gttagatttg cagaccatat aggaaatata tcaagcccaa aacaatatat attaggtcta
     4141 tttgataaga atcactttag gtgtataaaa gataaagcta agtataagga tatccttgat
     4201 agtatgtcaa aatgtatagt tgacgaggga gcttgttctc caacctatcc caaagatgta
     4261 tgggaaagag aaagttattt gagtactatt tatacaaatg gggtggctat acctcatcca
     4321 atcgagatga caggaaacaa aaatataata tcagtagctt tgatacaaac tgatattatt
     4381 tatgaaaata gggtgcctaa aattattttc atgatttcat taataaaggg aaatttagaa
     4441 cttcataagc aaatatctaa atatttaagt aagattatgg caaataagga tatggtagac
     4501 atgctaaata agtcacaatc ttatgaagag tttatgtata agttaaaaat ctatctggga
     4561 ggttagacat gaatatagag ttttttaaga ggttgtcaga agcagatggt atagcatcta
     4621 atgaagaaga ggttagacat gttttattgg aagaactaaa agcttatagt gataaaatta
     4681 tctgtgatgg gctagggagt atcatctttt ctaaaataaa agatgagagt gcaccaaacg
     4741 taatgatttg tgctcatatg gatgaagttg gatttatggt gagaagtata gacaaacttg
     4801 gtatgattca cttaataaca ataggaggag taaaacctct tgcacagttt gtacaaaaag
     4861 ttagaattac aacaaaagaa gggaagaaaa tccccgctgt cataaatgct acatataata
     4921 atggtaaggc tgaaaatata tatgcagata ttggagcata tacagaagaa gatgtatata
     4981 acctagggat taatgttggg gatatggtaa catatacaac aagctttgag gaatttactt
     5041 taccagaccg attagtggga aaggcttttg atgataggat aggatgtttt gtgatgggag
     5101 aagttttaaa agaacttagg aaagaaaatc taaattgtaa tattcacttt gcggctacaa
     5161 gtagtgaaga ggttggaata aggggagcaa agacatcaac acagttaata aatccagata
     5221 tagtatttgt aatagatgta gcatgtgcta aaaatgagtt tgtgagagac tatacaaata
     5281 agaaacaaat tgataaaggt gtaatgctaa tgcatagaga taggactcta gtccctagta
     5341 gaaaaatgat agattatgta atggaaattg ctgataaaaa taatatacca cttcaacatg
     5401 atatgtttga atcaggtggg actgatggag gagaagcaca tctcgtaaat gaagggaaac
     5461 cttgtgtagt aacttgtgta ccagttagat atggtcattg tgcattttca atagtaagta
     5521 ataaagattt ggagaacatt ataagattgt atacacagtt aattttaaat tttgatgaag
     5581 aacaatataa acatataaga aattttatct aaaggagaat aagaaaatgt caacagaagt
     5641 acaaataatg gaacttatat caactgcagg agagagtaaa tcgaaagctt ttgaggcttt
     5701 aaaaaaagtg aagtcaaaag attttaaagg agcaaaattg ttaatagagg aggctcgtaa
     5761 aatagatatt gaagctcaca ggatacaaac agaattaata actcaagatt taaatgaaga
     5821 tgttgaaagt ccagcaatga gtcttttaat ggtacatgct caagaccact atatgacttc
     5881 tcagctagca aaagacttga tagaggcttt gatatattgt cttgataagt aattgtaata
     5941 tagaaataga ttatttatgt aaaaattctt ttaaaagttt agatgctttt aagagataat
     6001 aaaaatacaa actttaggag gaattattat gaaaatatta ttatgttgtg caggtggatt
     6061 ttctactaat atgttaatgc aaaatatgaa aaaggtggta aaagagagtg ctaaactcaa
     6121 tgaaaaagat tttgagttta cagctatacc agcagattca atagaaagtg aagttgatga
     6181 ttgggatata gtacttatag gtcctcaaat agctcacaaa atagacttct taaaaggaat
     6241 attagagcca aaaaataagc cttttgcagt aatagataaa gatgtatatg gaaaaatgga
     6301 tggagctaca gttatgaagt tggcattaat aacttataag aaacatcaat tagctcaagg
     6361 ataataaaaa taatgaggga aaagggagat tagatatgtt ttcaaaattt gaagcattta
     6421 tgaatagagt atttatgcct ttagctcata aggttgacaa tcaacgtcac cttggttcta
     6481 taaaggctgg tatggttgct atgacaccat tcacaatatt aggaagtata tttgcaatac
     6541 ttccagcact tccaaatatg cttggagaaa ataatccaat aagcaaattc atacttagca
     6601 attcagcaat attggattta ccagtaagtc tttccatagg tttaatagga ttatatgctt
     6661 gtatgtgtat tgcttttaat ctggggaact actataaatt atatatacca ggttgtgttg
     6721 cactatcaac tttttctttt ttattcctag tagcctcatt tacaccagat ggagctttag
     6781 aaataggaaa tttaggagct aaagggctat ttacaggtat gatagcaggg cttggttctg
     6841 tggagttata taatttttgt aataagaaga atatcacaat aaaaatgcca gaaaatgtac
     6901 ctgattttgt atctaggtct tttgaattaa tacctactac tataatagtt tgtggaactt
     6961 ttatagttgc tcgttttatt tcattaaaag tatttggaga attacctcca cagatactta
     7021 ctcaattttt atctccatta gtaggaagta tggataaccc atggtcagtt cttgcactta
     7081 actttgctat atgtactata ttcttttttg gaatacattc atcagtattt agtcctataa
     7141 ctagacctat aatgattaca tttatagctg aaaacatagc agctatgcag gctggagaaa
     7201 caatacctca tttctttaca gcaggagctt caagtgcatt ctttggattt acaggatgtg
     7261 gaattagtat aggttgtgta attgcatgta tgttatcaaa aaatgcttcc tataagaaga
     7321 ttggacgtgt atcattattc cctgcattat ttgggataaa tgaaccaata ttatttggag
     7381 caccgataat attaaatcca ataatgttta taccacatgt atttggtgga gctataatag
     7441 gtacactacc aatgtttttt atgcactggg gatggttagc aaagccagta tttgaccctc
     7501 catatgtagg ggtattctta gaaggatttt taactaatgg agattggcat tctatattag
     7561 caaatgcatt gcaattagta ttagcagttt tattatattg gccattcttt aaaataatgg
     7621 aacgtcaaca aaataataaa aaacaaaaag aagaaactaa gaatatattc tctaaagaag
     7681 aagaaggctt attggatgat ttaggattgg atttctaata ttttgttatg aataatacaa
     7741 tggagagatt agtatcctta atctataaaa gaggtgttga cgctcttttg attaaagcaa
     7801 aaaatactaa aagatatata ggagctttat caggtagtgg tgtatatgtg cttgttacta
     7861 aggataaaaa ttatcaaatt ctagatggca gatatattga tgaagcagat aaaaaaacga
     7921 ctggtttcat aaaaaggatt gtatctcaag gttcttatat accaactata attgagttat
     7981 taaaagaatt aaatataaaa aagttagcaa tagaaaacca agctatgagt atacaagagt
     8041 atctttcact aaaaaatgaa ggttttgaaa tcaatttaat aacaaatgaa ttatggaagc
     8101 taagggctat aaaatctaaa gaagaaatta aacttattaa aaaggcttgt gagattacag
     8161 atgaagtatt tgatgaggtt atttctgaaa ttaaaaaaga tatgactgag ctagaagtat
     8221 cagctctaat tcagtatcat gcattaaaaa aaggtgcaag tggaatgtct tttgaaacaa
     8281 tagtggtatc aggagaaaga ggtgcaatgc cacatggaag acctacaaac aaaaaattga
     8341 aagtgaatga agctataaca atagattttg gtgttgttta tcaagggtat caatcagaca
     8401 tgactcgtac aatatctata ggcaaacctc ctaaaattat taaagagata tatgatgtag
     8461 tattggaagc acaattatct gcaatagagt caataaagga aggaactagg gcaagtgatg
     8521 ttgacaaagt ggctagaaaa attatagata aacatggatt tggcgaatat tttaatcatg
     8581 gactgggtca tggtataggt cttggagatg gtgaagttcc cactttaaat ccaaatagtg
     8641 aagatatatt agtggaagga atggtaatgt cttgtgagcc aggtatttat attccaaatg
     8701 tagggggagt tagaatagaa gatgatattg ttataataga tggaaaaggt gttccactca
     8761 ataagacatc caaagagttt ataatattag gagagtaaaa ttatgaaata taattttaat
     8821 aaagtattaa atcgtaaaga aggaaattgt agaaaatgga gcaaccatgt gattaaagaa
     8881 aaatttggct taaatgaaga tgcaattcct atggatttag ctgatatcga ttttgagtgt
     8941 gctcctgcta taaaagaatc tatcttaaaa agagcatcag taggagatta tagttatact
     9001 tttataggag atgacttcta tgatgctatt ataaattgga ataaaagaag atttgatgta
     9061 tatatagaaa aggattggat aaaattaact tttggtactg tttcaaccct tcattatata
     9121 gtccaagcat atactaaaga aggagagggt gtattgataa atacacctgc ttatgaccct
     9181 tttgcagagg ctgtagtaaa taataataga aagttgtgtt gtagctcatt aaaattagat
     9241 aacaatagat attatttaga tttagaggat atggaaaatc aaatgaaata cgagaatata
     9301 agattgctta tattctgtag tccacaaaat ccatcaggtc gtatatggac aaaagaagaa
     9361 ctatatcaag tgtcagagtt atgtttaaag tataatgtaa ttttggtaag tgatgaaatt
     9421 catagagata taatatttaa agattataga tttgtatctt tgtggaatgc tcatccagaa
     9481 atttataaaa atagtataat ctgtgtatct cctaataaag gcttcaactt aggtggatta
     9541 aaaacatcat atgtattaat aagagatgta aaaattaggg agactttatt agaaaggcta
     9601 aaaagtaatt caataacttc tccaaatgta tttgcaatac cagcaatagt tgcagcatat
     9661 aatgaaagcg aagaatggct agatgctatg actagttatg tagaggagaa ttttgaaata
     9721 gtttatgatt tctttgaaac taatatacca aaagctaaag tgatgaagtc agactcttct
     9781 tttttagcct ggatagatgt aagagaagta tttagaaatg aagaagaatc taaagaattt
     9841 tttagacatg caaacttaac tatggtagtt ggaagttatt ttgtgaaaga tggagatgga
     9901 tttattcgta ttaatgtggg gtgtcctaga gaaacattaa atgaggctct aaatagaatc
     9961 aaaaaaacgt atataagtat gtattgttaa atcataaaaa aagtatctat ggtttatata
    10021 aaaacacatt atttagttat caaatattac tggaattttg tattccagta atattttttg
    10081 attaaggaat attattgagg aatattaaat aatattattt cataccctta tattaactta
    10141 tataaaaata ttttattatt ttgggaacgg gttagctaac ctttatctag tgtatattta
    10201 tattgtaaga agcagtaaaa acttttcctt tactaagttt attaataaac ttcttatctt
    10261 tataaatttt gttagaattt atactatcag caagaccata ccaaggttct atacataaaa
    10321 aaggagcagt agaattagtt tcactataat aaggagtcca aataccaact agagggaaat
    10381 cttccataga tatagttaca tatttactac catcattatt acatatagac acttcatcta
    10441 tgttggtgta tatcaaagca tcattcttga atacttctgg atttaactgt aagtttttta
    10501 attttccaat aggtattata tcatctgtaa aagaaccatt taaattgatt ttttctacat
    10561 catttttact tttaaactca aggtagtgtt tagaaaaatc attttgttcg taaaaaggaa
    10621 ggttgaatgc aggatgtcca cctatagaaa agaatatatc tttagaatca gtatttttta
    10681 caatccattc tacctcaata ctattatcat ttaatgtata gtttatgatt agctcaaaag
    10741 aatatggata cttctttaaa gtagattcat tatctgttaa tttataagtt acaaatgtat
    10801 ctcctttatc tacaatctca aagtccatat ctctagcaaa cccatgttga gtcatgctat
    10861 ataaattttc ttctattatt gtttcattat caaaaagttt acctactata gggaacaata
    10921 taggtgattg tctaccccaa tattttttat taccatccca aagaatgttt ttgtcgtatt
    10981 ttttagaaaa tattttagtt agttcagcac caaaactgct tgattcgata attaaatttt
    11041 cattttgtaa tatattcatt taaaatctcc tttattatat tttatgagta tattatacta
    11101 taaagtataa agattattaa attttatttg atattatatt tgaaaggtag attttagtat
    11161 attttgaaaa ttttgttttg aaaatttggg attatttaag aaattttgta ttataaatag
    11221 aaggaaatag ataaattgta tagaataaaa tatattaaga taagttaata atgttaaatg
    11281 ggaagtgagt ttatggaaaa accaaataca aaagttgtta actttcaaga gtataaagac
    11341 agaaaaaacc aaagagaagt aaatagagac gaacttttaa agcttataaa aaaaattgtg
    11401 acttcaaaat agggagttgt taaactccct attttaatgg gtttcataat aattaagaca
    11461 ttctaagtcc atctatataa tctttgaatt gtgagaaatc tgattcacta cttaattcta
    11521 tggtttctga accttccgta taaaaattct taagatagat agtataatta ttatcaacac
    11581 aatcatgata aatacatcta tatccatctt catacaaact ttttaactta gaaaaatcat
    11641 ccacaatatc atctccttaa agttctttta aatatatttt tacaaaaagc atctaattgt
    11701 atgcaaattg acaatgtaaa ataaaggaaa tgaacataaa atgtagaata atttttatat
    11761 gtatgtaaaa ataaaaagaa aaagttgaaa tttttagaaa ataagagtat tttttatcta
    11821 aaaagagagt ttaaaaaata aaaaaatgtt gggaggataa aaaatgagtt tattagaaag
    11881 tatatcaaaa aacatatatt ccctagataa ctcttctatt gaaaaaacaa agcagagatt
    11941 ggataggctt atacatccaa ctggaagctt aggaaagata gaagatattt gcatgcaatt
    12001 agcaggaata tttggcaatg aaaattttga cacaagtaaa aaggttataa ttgcttttgc
    12061 gggagaccat ggtgtatatg aagaaggcgt agcacctgac cctcaaaata taacaaaatt
    12121 acagtttcca aatttttcga aaggtctatg cggagttgga gtaataagta aatttgtagg
    12181 ggcagatgtt gtagctgtag atgtaggaat aaactgtgat gaaaaactgg atggggtatt
    12241 agactacaag ataagaaaag ggacatcaaa tatggcaaaa ggtccagcta tgagtaaaca
    12301 agaagcaatt agatgtttag agataggtat tgaaatagca gagcagtgta tagaaaaaga
    12361 ttataaggtt attggtattg gggagatggg tatagcaaat actactccaa gtacagcaat
    12421 aatatctgta atttcagggt gtgacccgtt agaagttact ggtataggtg caggtcttaa
    12481 gaaagaaaga ctaaagcata aggctgaagt aattagaaag gctattgaaa ttaataatcc
    12541 aaatccaact gatggagtag atattttatc taaagtagga gggtttgaaa taggctcaat
    12601 ggcaggtgtt atattaggtt gtagtgctaa taggattcca gtagtaattg atgggtttat
    12661 ttcttatgca gcagcattaa ttgcatataa gataaatcca aaaactagag aatatatgat
    12721 agcatctcat ttatcagcag aatcaggaac taaaagagca ttagacatat taaaattaga
    12781 ccctctatta aatatggata tgagattagg tgagggaagt ggagctgctt tagcatttga
    12841 tataatagaa gcttcaaact atacttataa aaatatggca acttttgatg aaattgacat
    12901 gggaagataa atataaaaat ttaatatgta ggagtgtttt gatgagtaaa attattctag
    12961 taacaggagg agctaggtca gggaagagta actttgcaga aaaactatgt tttgacagaa
    13021 ataataatac agcttatata gcaacctcta ttccatttga tgatgaaatg aaagatagag
    13081 taaaaaaaca tcaagagaat agacctagaa actggaatac ttatgaaata tatgaagata
    13141 tatattccat aattaaggag atatataaag aacatgaaac tgttatacta gattgtgtaa
    13201 cattattagt aaataatcta atgtttgaac acaatctaaa tatagaagaa tctagtcaag
    13261 aagaaataaa caaattagaa caatacataa aagaacaagt tgtcaaactt attgaagaaa
    13321 tagaaaaaac aaatctatac tttgtatttg taacgaatga gttaggtatg ggaattgttc
    13381 ctgggaataa acttagtaga atttatgctg atattgtagg tcgtataaat caatatatag
    13441 cttcgaaaag taatgaagtt tattttgtag ttagtggcat agctatgaag ataaaggagt
    13501 agtatgcaat tatgaaaaga tttattttaa tactacagtt tttgacaagg atacctataa
    13561 aattaaatgt aggatttgat gatgaatttt ataagtcaat agtatatttt ccacttgtag
    13621 gttttgtcat aggtatatta tcctatttaa taggatggat ttctatgctt ttatttgaac
    13681 catttatagc ttctataata ataactctag ctggtgtctt aattactgga ggacttcata
    13741 tagatggatt aggagataca tttgatgcaa tatatagtta tagggataaa gaaaagatgc
    13801 ttgaaataat gaaagattca agattaggta caaattcact tctagcaatt atgtttgtac
    13861 ttttattaaa ggttggattc gtttacgata taataagtaa taattcattg tgggttataa
    13921 tttttatgcc aatgattgca aggttaggag taatgttatt gacatataaa actgtgacac
    13981 caagagaaaa aggaatggga aatttattta taggaaaatt aactacaagt atgttaataa
    14041 ctgctataat atacacatta ttgatagttg ctcttattac taaatttata tttttattac
    14101 ctaatatagt gttgattaaa gtattaggtt cgattatagt ggtttttgta tttataatat
    14161 tattcaaaaa acatatttat aaaaaaattg acggagtaac aggagatata ttaggctgtg
    14221 gaatagaact ttcagagctt gtatatttaa tttacatata tttgttaatt tttatgtttt
    14281 tttaaagtaa aaagtggtga taatatgata agattaatat taataagaca tgcactgaca
    14341 aatgataata aaaaaggaag attatcaggc catataaata gttgtattag tgaagagggc
    14401 aaactacaaa taaataaaat taccagatat ctgagtaatg aaaacataga taaggtttat
    14461 acaacaccat ctacacgtac taaagataca gtagaaaaaa tatcaaaatt gaaattatta
    14521 gaaatagaag aaaaagaagc tttaagagaa attagttttg gagactttga aggaagaact
    14581 tttgaagaaa ttaaaatcaa atatccaaat gagtttgaaa aaatgataaa agaaggtaac
    14641 aactatagat atccaaatgg agaaagtttg atagactctt ataaaagagt ggcaaaagaa
    14701 attgataata taattttaga aaataatagt aatttagata caaagacaat acttatatgt
    14761 tctcatgcag gaacaataag aaatataata acacatttga tttctggtag ttataagtat
    14821 cactggaact ttaaaattga taatgcatct attacagttt tagaaattga tggtggattt
    14881 gctgtaatag ataaaatgaa ttttacagac ttcatataat ttaatcaaaa ccaaacatta
    14941 caatctgtgt ttgtaatata tctgtaaaat tagcaatgaa aatagaatat tagattaaaa
    15001 ttaggttctt aattagatgt taaaagggaa aaaggttaga tgcctttgca gcccccgcta
    15061 ctgtgaaacc aacgaaacta taaataccac tgtcagtttg atgggaaggt tatagaagta
    15121 ggatgaagtt aagccaggat acctgcctaa tttaatttaa aacaagaggt cttcggggtg
    15181 aaagattttt aattaatata tataaaagtc ctatctttag aagtattagg acttttttta
    15241 atttgttttt tagtaaattt taacaaggag ggatggaaag tggggaaaaa aataatgcta
    15301 caaggaacag cttcaaatgt aggtaaaagt gtcttagtag caggtttgtg tagaatattt
    15361 aagcaagatg gttatactgt tgctcctttt aagtcacaaa atatggcttt aaattctttt
    15421 ataactaaag aaggattaga aatgggaaga gcacaagtat ttcaagcaga ggctgcgaaa
    15481 atagagccaa tagctgatat gaatccagtt ttattaaaac cttctggaaa taataaaagt
    15541 caagttattg taagaggtaa agttgtagga gaaatgcctt cttctaagta tcatgattat
    15601 aaattggagt tagtagatgt gttgaaggaa acatttaata gcttaaattc aaggtatgat
    15661 attgtagtta tggaaggggc aggaagtaat gcagagataa acttaaaaga tagagatatc
    15721 tctaatatgg gtatggcaga aatagctgat gcacctgtaa taatagtcgg agatattgat
    15781 agaggaggcg tatttgcctc attagcagga acaatgcttt tattaaatga agatgagaaa
    15841 agaagagtaa aaggtgtaat aatcaataaa ttcagaggaa aaaaagagct tttacaagat
    15901 ggtgtaaaga tgctagaaga catcataaaa gtacctgtta taggagtagt accttataca
    15961 gatataaaaa tagaagatga agacagtgta actacgagat ttaagaaaaa aatgaataaa
    16021 agtgatatac acatagaaat aattagaacc ccacatatgt caaattttac agattttaat
    16081 atatttgaga cacaagaaga tgtaagtata agatatgtag actatggaga atctttagga
    16141 aatccagata tattaataat acctggaact aaaagtacta tagacgattt aacatatata
    16201 agaaaaagtg gtcttgaatc gcaaatcaaa aatttacata gtcaaggtaa attgatattt
    16261 gggatatgtg gtggatacca aatgttaggt aaaaaattaa aagaccctta tcatgttgaa
    16321 agtgaaattg aagaagttga tggaataggt cttttagata cagaaacaac gtttgagaaa
    16381 gaaaagacta caacacaagt agaagctact attgttgaat gtatcagtga atacatgaat
    16441 aaccttaagg gaaaaaaagt aaaaggttat gaaattcata tgggaattac aaagcgaggt
    16501 aatgatgtaa gaagtttaga tttaataact aaaaaacttg ataaagaagt gaattatact
    16561 gagggaagtg taaataaaga ggggaatgta attgggactt atcttcatgg aatatttgat
    16621 gaaataagct ttacaagagc aattttaaac aatataagaa agaaaaaagg tttaggagaa
    16681 ttagaaagca aagtaaactc ttttaatgaa tttaaggata aagagtatga taagttggct
    16741 gattttttaa gagaacattt agatatagag aaaatatatg agattatgaa ataatgatga
    16801 cctttattta taatttaggt aggaggtgta ttatgaaaaa aattttaatt gcgggtacta
    16861 atagtggagt aggtaaaaca acaatatcat taggaataat gcaagctttg gttaagagaa
    16921 atatgaaagt acagccatat aaagtagggc cagactatat agacccttca tatcacactt
    16981 ttataactgg aagacattct agaaatttag attcttatat gttagatgat gaaaaaataa
    17041 agtatatagt aaataagtcc tcaaaagatg cagatatttc agtaatagaa ggtgttatgg
    17101 gcttatatga tggctttgga atagatttag ataattgtac aagttcacat acttcaaagg
    17161 tattaaagtc acctgttata ttagttataa atggtaaatc tatggcagca tctagtgctg
    17221 ctatggtact tggatataag gaactagata aagatgtaaa tataaagggt gttatagtaa
    17281 ataatgtaaa gactaatagc cattatcaaa taattaagag ttctatagaa aaatattgta
    17341 atgttgaagt attggggtat tttcctccaa ataacaaatt ctctttggaa tctcgacatc
    17401 taggattagt tcctagtgta gaaatgaaat cactaaggga aaaattttat accttagcag
    17461 atgaaattga acactatata gatattgaca gaataattga aatatcagaa agtgatgaat
    17521 ttgagagtga ttttgatttt caaactttga tagaaaataa taaaataaaa gaacatgtta
    17581 ataataaatc aatagctata gcatatgata aagcattcaa tttttattat agagaaaata
    17641 tagagctttt agaggaactt ggtttagaaa taaactattt tagccctctt gaagatgtat
    17701 cagtgccaaa tgcagattat atttatattg gaggaggttt tccagaaata tttggaaaag
    17761 aattagaagc aaatgagtca atgagaactt ccatattaga agcacataac aataatatac
    17821 ctatttatgc agaatgtgga ggactgatgt acctaggtga gaaactattg aatcaagaag
    17881 aacaagaatt taatatggtt ggaatattta gtggaattag taagatgaca tcttctttaa
    17941 agagatttgg gtactgtatt ggtgtagcca aggaaaatac tcttttatct aaaaaagggg
    18001 aatccattaa agggcatgag tttcatcatt caatatttga aagtgatgaa aaatgtgtat
    18061 attcaatgag aaaagaaaga gatggaaata tagtagaaga gtgggatggt ggttatagta
    18121 aaggaaacac tttggctaca tatcttcata ctcattttta taataactta aattgtatag
    18181 aaaatttttt gaaaaaagga aatgaataaa tgagtatttt atctatttat ataggatatg
    18241 tgacagactt aattgtagga gacccatatt cttttcctca tccagtgaga tttattggta
    18301 aacttataaa ttttactcaa ggtataataa gaaaggtctc taaaaatgat aggcagttaa
    18361 aatttggggg atttatatta tggtttataa ccgttggaat aacatattta acaacttatg
    18421 ctatagtgaa gttatttagt tttaatatat tattgagtat tgtagtgaat agttttataa
    18481 tttatacaac actagccaca aaatgtttaa aagacgaagc attaaaaatt tataatgtat
    18541 taaaaactga agatatagaa aaatctagga ttcagctatc ttatatagta ggtagggata
    18601 ctactaattt aagtgaacct gaaataataa gagcaactgt agaaactgtt gcagaaaata
    18661 ctgtagatgg tataattgcg cctatgtttt atgcatttat tggaggagca ccacttgcta
    18721 tggcctataa agctatcaat acattggact caacagttgg atataaaaat aaaaagtata
    18781 aagatattgg ttttgcatct gctaagattg atgatatagc caattatata ccagctagaa
    18841 tatctgtcat tcttatgaca attggaagtt tctttttaaa ttataactat aggaactgtt
    18901 ttaaaatctc tattagagat agaaagaatc ataaaagtcc aaattgtgct ttttcagaag
    18961 gtgcagtatc tggtgctcta ggaatacaac taggaggtac caacgtttat tttggggaaa
    19021 aagtatataa gccaacaata ggggataagc ttagggagat agataaagaa gatataatta
    19081 aaactaataa aattatgtat gcatcttcat ttgtatctat gttggtattt acactaatat
    19141 atgtgttatg taggatgatt ttaattaact taattatata aattattgtt tgttaactta
    19201 agggggacat agatgaagga tttagggcat ggtgccaatg tagatgaaat ggcaagatta
    19261 tatgggaaaa acccaaaaga aataattgat tttagttcaa acataaatcc aaatgtatta
    19321 ccaaatttag agagatacat tttaaaagga ttagaagagt gtaggaatta tccagatata
    19381 aattatacaa acttgagaga aaatatatca aaatatatag atataaatcc agactttata
    19441 atacctggca atggtgcaac agaagtgatt tatttgctta tgaagagtat taaaaaaaaa
    19501 ttggctataa taaatccgac tttttcagag tatagaagaa gtgctgagtt aaataattta
    19561 gatattatag atttagagtt agatttagaa aataacttta aacttaatat agatataata
    19621 aaagaaaata taaaaagatt tgatagttta tttatatgta atcctaataa tccgagtggt
    19681 aatgtccaag atttaaagga acttgttcat ttactagata aacataataa ggtactaata
    19741 attgatgaaa ctttcatgga atttgttgaa gatgaaagta aatatagttt ggttaaatat
    19801 atagaatcaa ataaaaatat atttataatt aaagctgtga ctaagttttt tggtatgcct
    19861 ggtttaaggc taggatatgg tcttacaagc aacactgaaa ttatgaacaa aatttatgaa
    19921 cacaaagagc catggactat caattctttt gcagatatac tatccaattt tatttttgaa
    19981 gataaagagt atattaaaaa tagtaaagaa tattacatag aagaaagaaa atatatgtta
    20041 caagagttga gaaacataag aaatataaag gtctatgata cagatgctaa ttttatcttg
    20101 ataagaatat ataaaaaaac aactaaagaa ttgaaaaaag atttgtttaa gcaaggaaac
    20161 atattagtta gagatgcatc taattttata ggattagatg atagctttat aagagtggct
    20221 ataaaatctc atgaagataa caaaattctt atagaaaata tgaaaaattt attgggtgat
    20281 taaaatgaaa tcttatggaa tatgcccagc atcttgtgga gaatttgtac aaggtataat
    20341 tgatgatgaa gaatatttat gttcatatgc aatagatatg tattctaaag tgtatattga
    20401 agaaaaatta gtagatataa atttagggag atataagtca aggttagcaa ttgaaaaggt
    20461 atttgaaaaa tttaatctac ctaaaaaata tactaaaaat atttctttaa atataaatag
    20521 taaaatacct gttggaaaag gaatggcaag ttcaactgct gatataggag ctactataaa
    20581 ggcaacatta tcattgatag ataaagattt aagtagcgag gagatttcta aattagcagc
    20641 agaaatagaa ccaacagatt ctatatttat agataaaaat agtatattta atcctctaaa
    20701 tggaactgtt ataaaatacc taggaaatct gaccaatgcg aaagttgtaa tacttgaacc
    20761 aaacaaagta ctagatacta tgaaaattag attgaggcaa gattataata aattaaaagt
    20821 agagaataaa gaagtaataa aaaaatcatt tgctctttta gaagaaggat tgaaaaaaaa
    20881 taatttatct ctagttggag aggcttgtac tttgagtagc ttagcaaatg agaatataga
    20941 aaaaaaagag tatttaaatg aaataataaa aatatcaaaa aaatatggag catatggagt
    21001 aaatattgct cacagtggaa ctgtagtagg tattttaatt gataagttta tgaatgataa
    21061 aaagatgata gatgccttat gtgaatctaa cataaattct gtctataata aaatttatac
    21121 acaaaacata attaatggag gaattaaggg ggaaatagaa tggaatacat aaagaatcct
    21181 atgaaaatag aagaaaaaag ttttgaattg atacaagaaa taatagacga aattagacca
    21241 gattataaat ttaagaataa tatcgaagaa aaaataataa aaagagctat acatactact
    21301 gctgattttg attacttaga tatattaaaa atatcagaag aagcagttga tagtataata
    21361 gatgcattaa aaaataatgc aagtatttat actgatacaa atatggctct tagtggtata
    21421 aataaaagaa agttagaatc attaggatgt aagtataaat gtttagtggc agatgatgaa
    21481 actgtaaaat tagctaaaga aaaaggaatt actcgttcta tggcagcagt agaaattgca
    21541 gctaaagaag aaggaagaaa aatattcgtt ttaggtaatg ctccaacagc tttatataaa
    21601 gttatggaga tgaaatcaga aggtagatta gatttagatg cagttatagg agttcctgta
    21661 ggttttgtag gagcacaaga atcaaaagat gaagttgaaa aaacagatat accatatata
    21721 atttctaaag gtagaaaagg tggcagtaat ttagcagcag caatagtaaa tgctatatta
    21781 tatactatgt aggtacctat atggaagaat atgtatatat agatggaaaa aaatatagaa
    21841 gaggatatac tacaggttca tgtgcaacag gtgcatctaa ggctgctgta tatatgttga
    21901 ttacaaaaaa tagaatcaat actataaata tagatactcc taaggggata ccattactac
    21961 taaaagttga taatataaat atttctgata catttgtgga atgttccata aaaaaagatg
    22021 gtggagatga tatagatgca actcatacca tggatattta tgcaagagct gaaattgtag
    22081 ctaaaaatga taagaacaaa gggtatctta ctctaaagga tatagataat ttaagcacca
    22141 atagtgagtg taaaagtgag ctctataagt ttattagggt ttatggcgga actggtattg
    22201 gtgtggttac aaaaaaaggt cttagcgtgg atgttggtaa acctgctata aatccgactc
    22261 cacttaaaat gataaatcat gaaataagaa agcttatagg agataatttt gaatctatac
    22321 ttggaaatga taaagtttta aaaataacaa tctttgctcc acaaggagaa actgtagcaa
    22381 aaaaaacatt taatccaaga ttaggtatag ttggaggaat atctataata ggtacaactg
    22441 ggattgttga acctatgagt gatgagggtt ggaagaagtc tctatctata gaacttcaga
    22501 tgaaaaaaga acaaggctta gataaaataa tattggttcc aggaaatcat ggagaacagt
    22561 ttataagaga aaaattaaac ttagatatta agtatgtagt tagggtaagt aattttatag
    22621 gctatatgat taaagaagct caaagaatag gatataagaa aatcttaatg gcaggtcata
    22681 taggtaaatt tataaaagta tctgctggta tttttaatac tcatagtaag gtagcagatg
    22741 ctagaagtga gatattagtg gcaaacttag ctttaatggg agctagatac gaatttttaa
    22801 ataaaataaa tcaatgcgta actactgaag aagctgtaga gctaataaat aatagtgaat
    22861 atagagaagt atataatata ttaagtaata aatgtaggga gagagtaaag caatacttga
    22921 atgaagattc agatgatatt gatgtggaag taatcatatt ttcaatggat aagtctttat
    22981 taggtaaatc ggataataca gatgatttag tggaggtctt tatatgataa acataatagg
    23041 tctaggacct ggtaacttag actatataac aaaaaaaggt gaaaatttaa tttccacttc
    23101 tgatgtatta attggtggta aaagaaattt agagtctata aaaaattttg aaggagaaaa
    23161 aatagttctg gattctaatt taagagaaat tatagagtac ataaataaca ataaagaaaa
    23221 gcaaatatca ataatagcat ctggggaccc actaatatat gggataggta gatatctatc
    23281 taaaaatatt gataataaga tgttaaacat ggtttctggg attagttcat tacagtacat
    23341 attttcaaaa atatatgtag atatgaatga tgtatatatt acaagtagtc atggtaaagt
    23401 accagatttt gattacatat tatcacataa aaaagtatgt atggttacag attcaaagat
    23461 aggaccaaaa caaataagta gagaaataat agatagaaat ctaaataaaa ttattgttgt
    23521 aggagaaaat ctgtcatatg acaatgaaaa aataacaata gcaaaaccag aagagataat
    23581 aagaatggac aattttgata tgaatgttgt ggtgatttta gatgaggaat agtgaattta
    23641 taacaggtaa agttccaatt actaaggaag aggttagagc aatatcaata agtaagttaa
    23701 atttagtaaa tgctaaaaac ctcatagaca taggggctgg tacaggaagt gtaagtgttg
    23761 aagctgcata taattatcct aatttaaagg ttatatctat agagaaaaat gatgatgcta
    23821 tagagcttat agagaaaaat aaaaaaaaat tcaacttgaa aaatatagaa gtgataaaag
    23881 gatatgctcc aattaatcta gggttaaatg ttaaagttga gtctattttt cttgggggaa
    23941 caggaaataa tctggaagaa ataatagaat ggtctaaaaa aaatctcgtt acaggaggaa
    24001 gacttgtagc taattttata ataatagata catttaatca aactttaaaa ctgcttaaaa
    24061 agcatggatt taaagaaata gatgtatgtg ttttgaatgt ttctaaacta gagaaactag
    24121 gaaaaggtga atattttaaa ccactaaatc ctatatatat aatatcttgt gaaaaagggg
    24181 aaaatgatga tgaataaagt acattttgta ggagcaggac ctggagataa agagctgata
    24241 acattaaaag gatacaaatt attaagtaat gcagatgtag tgatatatgc aggttcactt
    24301 gttaatcctg aacttttgga atactgtaaa gaagattgcc aaatacataa tagtgcacat
    24361 atggatttac aagagataat agatgttatg agagaaggta tagaaaataa taaatctgtt
    24421 gtaagactgc aaacaggtga tttttctata tatggttcaa ttagagaaca agtggaagat
    24481 ttaaataaat tgaatataga ttatgactgt actcctggag taagttcttt tttaggggca
    24541 gcatcatctt taggggttga atatacagtt ccagaaattt ctcaaagtgt aattataaca
    24601 agaatggaag gcagaactcc ggttccagag aaagaatcaa tacaatcata tgctaagcat
    24661 cagacatcta tggtaatatt tttatctgtt caagaaattg agaaagttgt gtctaaatta
    24721 ctggaaggtg gatatccaaa agatactcct atagcagtta tatacaaagc aacttgggca
    24781 gatgaaaaaa tagttaaagg aactttaagt gatatagctg taaaagttaa agaaaacaac
    24841 ataaataaga ctgctttaat aatggttggg agatttttag gagaagagta taataattca
    24901 aagttatatg ataaggattt caaacatgaa tatagagggt aatattgcta taatctgtat
    24961 tacagaaaat ggaaaaaaat tagctctaaa gataaattct ctagtaaata actgtgttat
    25021 atatcaagta aaaaataaaa aaagtaattt agttgttgaa gaaacatgtg tgcatacagt
    25081 tcaaaagaag ttaagtgatt ttgtaggaga aatatttagc aaatttgatt acatagtatt
    25141 tataatggct acaggtatag ttgtacgaag tatagcacca tacataataa gtaagtttag
    25201 tgaccctgca attttggtaa tggatgaaaa aggcaaaaac gtaataagtt tattgagtgg
    25261 tcatattgga ggggcaaaca gcttgacaca gtacataagt agtcttattg atgctaatcc
    25321 agtaataaca acagctactg atataaatca aaaagcttcc ttagatatga tagcaaaaaa
    25381 actaaatgca catattgata attttagaga aagtgtaaaa gaagtaaatt ctatgctagt
    25441 gaataataaa tgtgttggat tatatgttga tgaaggatat caagttgata ctaggggatt
    25501 taaacttata gaaagtaagg atgagttaag agatagtttt tcaaacacat atatagaaaa
    25561 attagttgta gtaagcaata aaataaattg tattgaagat attgagaatt taaacctaga
    25621 taataaattt gaacattgct atagtaccca aaaaataata aaggtaattc caagagatat
    25681 agtgatagga attggctgta gaagaaacac agatagtgaa ctgatgtata attcacttga
    25741 agagtttctt aataaacaaa atatagatat tagagcaata aacacagttg gaagtataga
    25801 actgaaaaaa gacgaaaaag caataattga attatcaaaa atattgaagg tgccatttaa
    25861 aacattttca gtagatgaaa tatctaaggt tgataatctt tttgataaat ctgattttgt
    25921 taaaaaaaat gtgggtgtgt attcagtagc agaacctgta gcatatcttt tgagcaaagg
    25981 taatttgtta gttgaaaaac ataaatataa aggaataact ttttcagtgg ggagaataaa
    26041 aatatgatat acgttgtagg aattggacca ggaagtaaag atacaatgac tttagaagca
    26101 ataaaagcaa tagaagattc tgaggctatt gttggatata agacttatat aaaactaata
    26161 gaagaattta tacaaggtaa agaaataatc caaaatggta tgaggcaaga agttgataga
    26221 tgtaaacaag cagtggagga agcaaaaaaa ggaaaaaaag tagcagttgt aagcagtggt
    26281 gatgctggaa tatatgggat ggcaggtctt attttagaat taatatctaa agaagatgaa
    26341 gatataaaag taaaggtaat tccaggcgtt actgcaagta ttggtgcagc agctatactt
    26401 ggagctccta ttatgcatga tttctgtcat ataagtttga gtgatttaat gactccgtgg
    26461 gaagtaattg aaaaaagatt gagattggct gctgaagccg attttgtaat ttgtttgtat
    26521 aatccaagaa gtaagggaag aagtgaacat ctaagcaaag cattcaaaat aatgggtgag
    26581 tttaaaggtg gagatacacc tgttggagtg gtaaaagatg taggtagaga aaaagaagaa
    26641 aagtttgtat gtacttttga aaatatggac tttgaaaaag tcgatatgac tacaatggtt
    26701 ataataggaa ataagtctac ctatattaat ggtgaattga tgataactcc aagaggttat
    26761 acagtatgat tttaatttta ggaggaactt cagatagttt atctatatgt gataagataa
    26821 atgaactgaa aaatcaacca tatatccttt cagttactac agattatgga agggaacttg
    26881 cacaacaaca tgcagagaat gtaattttag gtaagcttgg aaaagaagat atgctaaaat
    26941 ttataaaaga taataatata gtaaaaataa tagatgccac tcatccttat gcagttgaag
    27001 tatccaaaac tgctattact tgtgcaaaac tattgaatat agattatata aggtttgaga
    27061 gaaaatcttt aattgaagaa atagattatg ataataaatt tatagtagat acaattgaag
    27121 aagcctgtga aatagcaaat aaaataggga caaatatatt cataggaaca ggaagtaaaa
    27181 atttgaacag atttatagag gaaataagtg ataaaaattt agttgttaga gttcttccaa
    27241 cgagtgaagt catattaagt tgtgaaaaat taggtttaag tgcagataat atagtagcta
    27301 tgaaaggtcc atttagccag tatataaatg aagaaacata taaacattac aatatagatt
    27361 tagtcataac aaaagaaagt ggaatagctg gtgggttctt agagaaggta aatgcatgta
    27421 aaactttcaa tataccagta ataattataa aaagagaatc gattaactac gaaaatgtat
    27481 ttaatgacat aaataaaatt aaagacatac ttgtgtaatt tttaggagga gaatatatga
    27541 agaaagcctt attagtagtg agttttggta caagctatca tgaaacgaga gaaaagacaa
    27601 tagatatatg tgaagataaa ataaaaaatt cgttaaaaag ccatgatttt tttagagctt
    27661 atacatcaaa tatgataata aataaaataa aaaaaaggga tggaatagaa atagataatc
    27721 caatacaggc tttagataaa atatatgaac aaggatatga tgaagtaata atccaaacac
    27781 ttcatattat atgtggcgaa gaatttaaca aattaaaaga acaagtagat ggatattctt
    27841 ctaaattcaa aaagattgta ttaggaagac ctttgcttac acatattgaa gattatcagg
    27901 aagctgtaga ggcaattaag catcaaattc ctcatatgga aagtaatgaa gcagtagtat
    27961 ttatgggaca tggaacactt catgaatcac attcagcgta tccagcattg gaatacatgc
    28021 ttagggataa tggaataaat gcttatgttg gtacagttga agggtatcca gaaatagaac
    28081 atgtaataag aagattaaaa gaaggaaata taaaaactgt aaatttgatg ccatttatgt
    28141 tagttgcagg agaccatgct ataaatgata tggcaggaga tgaagaagat tcttggaaaa
    28201 caatattaga agaaaatgga tttaacgtaa gagttcattt aaaaggacta ggagaaaatt
    28261 cttatataca agataaattt gttcgtcatg ctgtaaaatg tgcagagaat gcgaagttct
    28321 atggtatagg agcagggcca ggagatggtt ctttacttac gataaaagcg gttaatactt
    28381 taaagaaact ggatattcta tacactccag aatcaaaaaa gggtggagat agtctagctt
    28441 tatcaatagt tagtgaatat ttacctgaat cagtggaaat aaagtctagg catttcccaa
    28501 tgagttttga tgggaatgaa aaaagtttag cttgggacaa tgtggcacaa gaaattatat
    28561 cagatgttaa tgatggtaaa aatgtaggat ttgttacatt aggagaccct atgatttata
    28621 gtacatatgt atatataatg aaaagaatat taggagaggt agaagtcgaa actataccag
    28681 gaatttcatc attttccaat atagcatcta atcagaattt tccattagtc atggataaag
    28741 aagctttagt ggtaattcct tgtactatgg aagatgataa gatagagtat gctttgcaaa
    28801 attataattc aatagtattg atgaaagtat ataaaaattt taaagaaata ataaaaaaat
    28861 tagaaaagta tgatttaata gaacatgcta ttttagtaag taattcttcc caagaaagcg
    28921 aaaatgtgtt tacagattta aaagaagcgc accttgaaga taagatatct tatttttcta
    28981 ctatactagt aaataaagat aataaaataa aataggtgaa agcaatattt aaaggagact
    29041 taatatgaat atagtggttg gaactagggg tagtaatcta gcgcttatac aaacagaatg
    29101 ggtaataaat gaattaaaaa agaagtatcc agaaattagt tttgagatta aaatcataaa
    29161 gacaaaagga gatttaattc aaaatgtttc actggataaa ataggtgata aaggattgtt
    29221 tgtcaaagaa atagagcaac aattattaga tggaaaaata gacatagcag tacatagtat
    29281 gaaagatatg ccttcttatt tagcagatgg actaaaattt gctcatacgc ctaagagaga
    29341 agacccaaga gatgttctaa tacttagaga aggatataaa aatttagatg atttgcctca
    29401 tggagcagta ataggaacag gaagtaaaag aaggaagttt caattattga aacaaagacc
    29461 tgatttaaat atagtacaag ttagaggtaa tgtggaaact aggatacgaa aaataaaaga
    29521 tgaaaacatg catggtattg tgttggcagc atcaggaata attagagcta atttacaaga
    29581 taaaataagt tcttatttac ctgttgatgt tgttattcca gcacctgccc aaggagcttt
    29641 agctatagaa attagaagca atgatagtac gatagaggga atagtaaatt cactgaagga
    29701 tgagaataca gaaatccaaa tattggctga aagaggtttt ttagatggtg taaatggaag
    29761 ttgtcatatt ccaatggcag cttattgtga aataatccaa gataaaatac atcttactgg
    29821 cttatatgga gattcagaag gaaaaaaagt agtaatcaag tctatagatg gagatatttc
    29881 ttctcctaga gagttaggtt taaagttagc taaacttgta ttgaaggagt atgagaatta
    29941 tgaagggtaa ggtttactta gtaggggcag gaccaggtga ttataaactc atgactttaa
    30001 aaggtttaga gtgtataaga aaatcggatg taatagtata tgatagatta gcaaatagta
    30061 gttatttaag agaagctaag cctgattgtg aacttatata tgtaggcaag gcatcaagta
    30121 atcatatttt gccacaagaa gatataaata gaattatagc agaaaaagct aaagaaggaa
    30181 aaattgtaac tcgtcttaaa ggaggagacc cttatgtatt tggcagaggg ggagaagaag
    30241 cagagacttt agtagatgaa ggtatagaat ttgaagtagt tccaggtata acttctgcta
    30301 taggaggttt atgttatgcg ggtattccta taacacatag agaccatgct tcgtcatttc
    30361 atgtaataac aggtcatttg aagggtgatg atagtggaga attaaactgg aatgctcttg
    30421 caaataacaa aggtactctt gtgtttttga tgggaatttc aaacctagag aaaataagtg
    30481 aaaacttaat gaaagaagga aaagataagg atacacctgt tgctcttata agttgggcaa
    30541 ctagatataa tcaaagagtt gttacatcta cacttgaaaa tgtttatgaa actgctatta
    30601 aagaagaagt aaaaccgcca actcttatag tagttgggag tgttgtagga cttagagaaa
    30661 agttgaactt ctttgaaagt aagcctttat ttggaaaaag tattgctgta actaggtcta
    30721 gaaatcaaaa tagtgtattg gtagaaaaaa taatggattt aggtggaaat cctatagaaa
    30781 tacctactat taaggtagaa aaaatacaaa ataatattaa cttagaaaat gaaataaaaa
    30841 atataaataa gtataattat ctaatactaa ctagtaaaaa tgccgtagaa atcttctttg
    30901 aaaagatata tgaaatgaat tttgatttaa ggatattatc taatttaaag gtatgtgcta
    30961 taggttctgc tacatcaaat gagttgaaaa aaagaggaat tatagcggat atagtaccaa
    31021 aaaaatttgt agcagagtca ctgtatgaag agttagcgcc tattttaaat gatgacgata
    31081 atatacttat acctagagca gaaaatgcta gagattatct ggttgacaaa ctcaaggcta
    31141 tttgtgaagt aacagaagta catatatata gaacagtaat agatgaggag aaaaaaggtg
    31201 agatattaga tattttaaat tctaatgaca tagattatat aacatttact agttcctcaa
    31261 cagtaaataa tcttgttgaa attataggaa aagaaaatat taaaatatta gaaaaaataa
    31321 aaacactatc tataggtcct ataactacag agacaataaa tagtttggga ataaaattgt
    31381 acaaagaatc tgctacatct actatagatt cgttagtaga tttaattata aaaaattaat
    31441 tttggaggct tgaatatgtt aagaagacca agaagactaa gagcaagtaa agcaatcaga
    31501 aatttggtaa gagaaactaa ggtgaatgta gaagacttaa tatatccact gtttgtagta
    31561 gagggggaaa atataaaaga agaaattaaa tctttaccag atgtctatca tttatcaata
    31621 gatatgctag aagacgaaat aaaagaaata agtgagttgg gaattgaaca tataatgtta
    31681 tttggtgttc cagatgaaaa agatgagtgt gcttcacaag cttttaatga taatggaata
    31741 gttcaaaagg caattagaaa ggtaaaagaa ataaattcag atataaatgt aataacagat
    31801 gtctgtatgt gccaatatac aagtcatgga cattgtggga ttttaactga tactggttat
    31861 gttgataatg ataaatcatt ggagtatttg gctaaaatat ctgtaagcca tgctaaagca
    31921 ggagctgata tggttgctcc atctgatatg atggatggaa gaatacaagc aataagagaa
    31981 gctttagatg aaaatggatt agaaaatgtt ggaatcatgg cttatagtgt taagtatgct
    32041 tcaaattatt atggaccttt tagagaggca gctcattcag caccatcttt tggagataga
    32101 aaaacatatc aaatggaccc agcaaatagc aatgaagcta taatagagac tgaacttgat
    32161 atattggaag gtgcagatat acttatggtt aaacctgcat tatcatattt agatattata
    32221 agaagagtta aagataattt tgacttacct ctagcagctt ataatgtaag tggagaatac
    32281 tctatgctta agtttgctgt gaaggaaggt ttattaaatg aagatgtaat tcttgaatca
    32341 attacatcta taaaaagagc aggagcagat ataataataa cttattttgc taaagacatt
    32401 gctaaactgt tgaaaaaata aattctagca gtataaagaa tatcaaaaag tataggaatt
    32461 gtaattaact attatacagt agttttgttt agacatttat tgaagaataa tagaaactaa
    32521 tttgcaattt ctatacttta tttatgtaac taaataaaat ttggaggtag aagatgctat
    32581 atcctattaa tttaaaatta gatgagttag atgtagttat aattggtggt ggagaagttg
    32641 catataggaa atgtaaaaat tttttagagt ttaacaaaaa tgttactata gtttctaaac
    32701 aaattttaaa taaattttat gatttaaaag gaaatataaa aataataaaa gatgattata
    32761 aagaaatata tataaaagat gcttctgtaa taattgctgc cacaaataat agagagttaa
    32821 atatggaaat aggattatat tgtaaagcaa aaaataagct agtgaatgta gttgataata
    32881 tagaaatatc gaactttact gttccatcat atataaaaag aggagattta cttataagtg
    32941 tgtctactgg gggcaagagt ccttcattat catcaaagat aaaaaaagaa attgaagaga
    33001 gatatactga agattatgag gagtatttaa atgtattagg agatataaga aaaaaggtta
    33061 taaaaaaata cgaagataaa agtaaaagaa aagatgtgct taatatgttg gtaacacttg
    33121 acctagaaga acttaagaag tttgatatat aaaaaataaa ataaaaaatg ttaaaaaggt
    33181 gttgacggat tatttttaag atgatatagt attacttgtc cttaagaaaa gggcaaacat
    33241 aaaaaa
//
LOCUS       DAYAOY010000042        32646 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYAOY010000042 DAYAOY010000000
VERSION     DAYAOY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.042"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(9..860)
                     /locus_tag="ACGI6B_002021"
     CDS             complement(9..860)
                     /locus_tag="ACGI6B_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9751464.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            1077..2243
                     /locus_tag="ACGI6B_002022"
     CDS             1077..2243
                     /locus_tag="ACGI6B_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HFG9751465.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            2265..3911
                     /locus_tag="ACGI6B_002023"
     CDS             2265..3911
                     /locus_tag="ACGI6B_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFG9751466.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            4008..5381
                     /locus_tag="ACGI6B_002024"
     CDS             4008..5381
                     /locus_tag="ACGI6B_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9751467.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5826..6779
                     /locus_tag="ACGI6B_002025"
     CDS             5826..6779
                     /locus_tag="ACGI6B_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFG9751468.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6817..7779
                     /locus_tag="ACGI6B_002026"
     CDS             6817..7779
                     /locus_tag="ACGI6B_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFG9751469.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7804..8790
                     /locus_tag="ACGI6B_002027"
     CDS             7804..8790
                     /locus_tag="ACGI6B_002027"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFG9751470.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8803..9849
                     /locus_tag="ACGI6B_002028"
     CDS             8803..9849
                     /locus_tag="ACGI6B_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFG9751471.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            10016..11746
                     /gene="lpdA"
                     /locus_tag="ACGI6B_002029"
     CDS             10016..11746
                     /gene="lpdA"
                     /locus_tag="ACGI6B_002029"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFG9751472.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12164..15112
                     /locus_tag="ACGI6B_002030"
     CDS             12164..15112
                     /locus_tag="ACGI6B_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751473.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            15090..15554
                     /locus_tag="ACGI6B_002031"
     CDS             15090..15554
                     /locus_tag="ACGI6B_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9751474.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15667..15969
                     /locus_tag="ACGI6B_002032"
     CDS             15667..15969
                     /locus_tag="ACGI6B_002032"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9751475.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            16046..17395
                     /locus_tag="ACGI6B_002033"
     CDS             16046..17395
                     /locus_tag="ACGI6B_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9751476.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            17430..17903
                     /locus_tag="ACGI6B_002034"
     CDS             17430..17903
                     /locus_tag="ACGI6B_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751477.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            18075..18725
                     /locus_tag="ACGI6B_002035"
     CDS             18075..18725
                     /locus_tag="ACGI6B_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFG9751478.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18899..19831
                     /locus_tag="ACGI6B_002036"
     CDS             18899..19831
                     /locus_tag="ACGI6B_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFG9751479.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            20105..20818
                     /gene="ispD"
                     /locus_tag="ACGI6B_002037"
     CDS             20105..20818
                     /gene="ispD"
                     /locus_tag="ACGI6B_002037"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFG9751480.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20815..21300
                     /gene="ispF"
                     /locus_tag="ACGI6B_002038"
     CDS             20815..21300
                     /gene="ispF"
                     /locus_tag="ACGI6B_002038"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFG9751481.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21395..21603
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21667..23382
                     /locus_tag="ACGI6B_002039"
     CDS             21667..23382
                     /locus_tag="ACGI6B_002039"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9751482.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23504..24949
                     /gene="proS"
                     /locus_tag="ACGI6B_002040"
     CDS             23504..24949
                     /gene="proS"
                     /locus_tag="ACGI6B_002040"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9751483.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            25387..26868
                     /gene="gltX"
                     /locus_tag="ACGI6B_002041"
     CDS             25387..26868
                     /gene="gltX"
                     /locus_tag="ACGI6B_002041"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFG9751484.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            27089..27244
                     /locus_tag="ACGI6B_002042"
     CDS             27089..27244
                     /locus_tag="ACGI6B_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751485.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            27389..28786
                     /gene="cysS"
                     /locus_tag="ACGI6B_002043"
     CDS             27389..28786
                     /gene="cysS"
                     /locus_tag="ACGI6B_002043"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFG9751486.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            28789..29184
                     /locus_tag="ACGI6B_002044"
     CDS             28789..29184
                     /locus_tag="ACGI6B_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HFG9751487.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            29290..30048
                     /gene="thyX"
                     /locus_tag="ACGI6B_002045"
     CDS             29290..30048
                     /gene="thyX"
                     /locus_tag="ACGI6B_002045"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HFG9751488.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            30061..30798
                     /gene="rlmB"
                     /locus_tag="ACGI6B_002046"
     CDS             30061..30798
                     /gene="rlmB"
                     /locus_tag="ACGI6B_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFG9751489.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            30801..31328
                     /locus_tag="ACGI6B_002047"
     CDS             30801..31328
                     /locus_tag="ACGI6B_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFG9751490.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            31393..32040
                     /gene="sigH"
                     /locus_tag="ACGI6B_002048"
     CDS             31393..32040
                     /gene="sigH"
                     /locus_tag="ACGI6B_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HFG9751491.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            32114..>32646
                     /locus_tag="ACGI6B_002049"
     CDS             32114..>32646
                     /locus_tag="ACGI6B_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFG9751492.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT        13268 a         3436 c         6176 g         9766 t
ORIGIN      
        1 atataacttc atattttagg tagatttttt cttatatgtt taggagtgca tttaaatttt
       61 tccttaaaaa ttctgtaaaa taattttgta ttagtaaatc catgagcttc taatatactg
      121 cataaggtat catttgtatt aattaagtct ttataaatat gagaaatacg cacatcattt
      181 aaatactcta agtaagtttg tcccatatac tgtttaaatt ttctacaaaa atattctggt
      241 tgtaagtgtg ctaccttagc gattttgtta atagaaatag gcattttgta attaatcttt
      301 gtatattcta aaactggttc aaattttgac aaatcaagta ctgttttttt catattttta
      361 ttacctacag atattttaaa gttagtatat aattcaaata aaagttcaaa taataagctt
      421 gtaaagagca gattagctgc aggaggagaa ctaagtttaa tttctctcat gttttctaat
      481 ataagtttaa ctttacttga atttttttta tagttattgt cattggcatt aaccttaaag
      541 tcaaaatagc aatttttaaa atcagggata tatttgttta gaaaagacaa tggtacttgc
      601 aataatatag aggtattttg ataaatacat ctagttgagt gaataattcc agaattaatg
      661 aacatacact cattagcttt taaattataa gtgtaaccct ccatgttaat ttgtaaatca
      721 ccagaagtta tataaattat ttctaatgag ttatgccaat gatttgctac ataactacca
      781 ctatcatcaa atagtttgaa attaacatct aaattatcaa taggttcaat tatttcatgt
      841 gaaaaattat ttctgttcat aataatatcc tccttataat tttaaattat aaatagtaaa
      901 tttataatgt tcaatattaa aaagataata tagattaata aattagataa taaacatact
      961 taagaaaacg gataaaatca aatataataa ctgtatatta aaataactta aaaaaattat
     1021 tttattataa gtgcaaaata tacctattta aataagataa gaggggagga ttttatgtgt
     1081 atataaaagg tgtaaattta ggaggttggt tagttttaga aaaatggatg acctcaagtt
     1141 tatttgaagg cacagaagct gaagatgaat actatttacc aagacagctt tcaagagagg
     1201 catatgaatc tagaattaag actcatcgta gtgaatacat cacagagaga gattttgcaa
     1261 tgattaaatc tatgggattt aattcagttc gtataccagt accatacttt atttttggag
     1321 attgtgaacc attcatagga tgtgttaaag aacttgataa agcatttgct tgggcagata
     1381 agtatgggct tagtatattg atagacttac atacagttcc aggaagccaa aatggatttg
     1441 ataatggagg aatatcaggt atatgtagct ggtcacaaaa tccagaatat gtagcgttta
     1501 cattaaatgt attagaaaga cttgcaaaac gttatgggat gaggcatgaa ttgtatggaa
     1561 tccaaatttt aaatgagcct ataacagaaa gaatgtggaa cataatgaat gttccaaatc
     1621 ggtttaaggc tgtagataag gagatggcaa gggggagtaa acctaattca ttagagtttt
     1681 tacgtgattt ttatataaaa gcatataagg taatgcgtcc ttatatgagg gaggaaaacg
     1741 ttatagtatt tcatgatgca tttgaattaa aagcatggaa agactttatg agagaggaag
     1801 aatttaaaaa tgttgttcta gatacacacc aatatttgat gttagctgaa gcagatggat
     1861 gtgaacaaag tattgatagc tatctcaaat atattaggga aaattatgct aaagatattt
     1921 tacaaatgca aaaatatttt cctgttatat gtggggagtg gagcttattt aattcatatg
     1981 cttgtggtat agatacaaat ggaggtcaat cacctctaaa tgggattgaa tcaaatatag
     2041 ataaattatc aaaagacgat aaaagagaat tatataggaa gatagcaaaa gcacagcttg
     2101 atgcatggag aaatggaagt ggtcactact attggaatta taagttatta ctagatacag
     2161 ttaatgaaga aggttggata ggatgggata gttgggacct agtcaaatgt gtagctcaag
     2221 aatggtatcc tatagaatat taaactaaaa ttggaggaaa agatatgatt aaaaacccta
     2281 tacttccagg ttttaatcca gatccttgta tttgtagaaa gggagatgat tattatcttg
     2341 tagtttcatc ttttgaatgg tttccaggta tacctgttta tcactctaaa gacttaaaaa
     2401 attgggagtt atatactcat attttgacag atgaaacaaa aatagatttg aagaagcttc
     2461 cttcatctaa aggaatttgg gcaccctgtt tgacttattg tgaagaagaa gatttatttt
     2521 atatagttta tggaattatg aactctatga atgcaagata ttttgatgtt gataattatt
     2581 taattacatc aaaagatata aagggagagt ggagtgaacc agtatatcta cactcatcag
     2641 gttttgatgc gtctattttt catgatgatg atggtaaaaa gtggattgca tcattagact
     2701 gggagacaag agaaggatat gaaaaacctg gtgttatatg tttagtggaa tactgtacaa
     2761 aaaagaaaga aatagtagga tatccaaaga gaatatggtc agggggaact gatagaggtt
     2821 gtattgaagc accacatata acaaagcgtg gagattacta ttatataatg tgtgcagaag
     2881 gtggaactgg atatggtcat agtgtaacta tgggaagggc taaaaatata tggggacctt
     2941 atgaaaaaga ttcaatgaat ccaattgtaa cttcaattcc aggcgatttt tatgaaagac
     3001 atgaccctga ccatctaaag cctaaatact ataatccaga atcaaaactt caaaaatctg
     3061 gtcatggaag ctatatagaa acaacatcag gtgaagtata tttagttcat ctaacatcta
     3121 gaccatttgt accagagtta aggtgtactt taggtagaga aacagctatt caaaaaatga
     3181 aatggacaaa ggataattgg cttcgaatgg aagatgaaag taatttggca aaagaatacg
     3241 tatcagaaag taaactagaa gaacatttag tttcatctat accaagtttt gatgactttg
     3301 attcaaatga attaggttta cagtactatg ctcctaggat ttcaccatta tcatttgcag
     3361 atgttaagtc tagaccagga tatgtaagga ttagaggaca agaatcaaga acatcattaa
     3421 ataaagtgag catattggca agaaaattaa cttcagtata tgcaagaata accactaaga
     3481 tggagtttta cccagaggta catcagcata gtgcgggttt gataatgtat tatgataata
     3541 tgaattatat aaatttaaga aagtattata gtgaaacatt gggtcaaagt gcactatcaa
     3601 taatacatct tgaaaatgga gagaaaacgg aatttttaaa tactaggata ccaataaaag
     3661 atataccaat atatcttcgt ctgtatgtac aaggtcgtaa gtcatatttt gaatggagtt
     3721 atgatgaaaa aaactaccag agaataggaa aggtatttga tacaactaag ttttcagatg
     3781 agtactgtaa atatggagaa tttacaggta cgtttatagg attaacttgt gcagatcgtg
     3841 taaagcataa acactatgct gattttgatt tttttgaata tatagtagat gaatcaaaag
     3901 atgtcgatta ataaaatcat tactaaaatt ttcagagcaa aggtaaatct tatgctctga
     3961 aataagaaac attagaaggc aaatcttaca aaaaggagta tggtattatg aatacagaag
     4021 ttaatgttaa agcaactacg attagatatg aaaagatttc attttttgaa agaatttctt
     4081 atggttgtgg ggatttgggg tgcaatatca tatactcagc tatgtcagca tttttgctgt
     4141 tttattatac taattatgca gatgttagtg cagcagctgt aggtagtatt atgcttgtat
     4201 ctaggatact ggatgggttt agtgacttaa caatgggaat tatagtggat agaactaaat
     4261 ccaaatacgg taaggctagg ccttggattt tgagaatggc gattcctttt gctatagcag
     4321 cagtattatt attttctgtt ccatcaaatc ttggtatcac ttctaaatta atttatattt
     4381 ttattacata taacttagtt tctactgtta tttatacagc aatcaatgta ccctatgcaa
     4441 ctctaaattc acttataact caaaaccagt atgaaagagg agtattaagt atatttagaa
     4501 tgatattagc cacttgtggt accttactaa taaatggact tacactacct ctagttgaat
     4561 actttggaaa taatttatca gcatggacta aaacattctt tgtatttgga atagcatcta
     4621 taatggtatt ttttataaca tttactggaa ccaaggaaag agtaaaagca gttaagcaag
     4681 ataggaatga agtaatacca tttaaaatag gtattaaatc tttatttaga aataagtact
     4741 ggatacaaat aacattatgt ttagtttgca tatttattgt atttgcgata aatggtggtt
     4801 catccgtata ttatgcaaag tttattttgg gagatgaaaa attatttggt cctataaata
     4861 tggtttcaaa tatatcccaa attatagcta tgtttatggt tgctcctttt ataaaaaaat
     4921 ttggaaaaag aaatgtatta atagtaggtt caataatatt aatatcgtca aatgttatgt
     4981 ttataatagt tggacaaaat catataggag taatatttgc aagtgctata aagggaatag
     5041 gaagtgctgg aatagcatca acaatgtttg ctattgtttc agatactatt gaatatggag
     5101 aatggaagac tggatataga actgaggggc ttataaatag tgcatctagt tttgggttta
     5161 aagtgggaaa tggattaggt tcagcaatat taggtgcagt attatcaatt ggtggttatg
     5221 taggaacatc agctactcaa agtgatttag ctatagtatc aataaaagta tgttttatat
     5281 acctacctat atttataaca atattacaaa ttatcataat gtctttttat aagcttgata
     5341 aagaatatag tactatactt aatgaattaa atactaaata atgaaaaagt ttcttataaa
     5401 gtcttaagtt taattaaggt ctattgtaaa tgttccctat aaaaactatt atatatttat
     5461 gattattgaa gaatttttag agaagcgaat aataaaaata taagaagact ttaaatgatg
     5521 aaatggttgg ttttttagaa gtgatttaat aagttattgt tatttaaagc attataagtg
     5581 gacaattaat ttttaattta tggagatgat atttaacaat tagaagtaat atataatatg
     5641 cactaagaag caatttttca actatttatg aaggtggtgg tacaagaaaa gtatcacctt
     5701 ttttttgtta tacaaaaaaa cagttgactt tttatatgaa ccccttctat aatgttactg
     5761 aaattaagta aaatgattga acaaatgtaa agataattga aaagcttgat tttggaggaa
     5821 agcaaatgaa aaaaattgga gacttaagat taatgatgaa gtgttgttca ctttattacg
     5881 aagataattt gaatcaacag gaaatagcaa atcaattggg aatatcaaga ccaacaatat
     5941 caagaatatt aaaagaagct tttgaacaag gaatagtaaa aatacagata gttgatgttt
     6001 tgaaaaatga ttaccaaaaa atagagagaa gtttggagag aaaatataaa ttaaaagaag
     6061 taatagtagt agatgataaa caggatgcac taacacaaaa acaagaacta gcaagagctg
     6121 tatctgaata tttaactaga gtagtaaagg aaaatgatat aataggtgtt tcaataggaa
     6181 caacactaaa agaaatacca agatatgtgg aaaaaagtaa ctgtaaaaat gttacattta
     6241 tacctctatt aggagggatt ggagacaatg aaatagatat tcatgcaaat caaatagccg
     6301 taagtttagc gagggctttt ggaggtgatt ttaaattatt acatgctcca gctgttatgt
     6361 ctgacttgtc tacaaaagag aaactttgca aagacgaaaa aattaaagaa gtactagatt
     6421 taatagataa aacaactata gcaattgtgg gtataggaaa tcctatgagt ttaaactcaa
     6481 caataatggc ttctggatac atgaatgaag cagatataga agatttaaaa aaatacaact
     6541 caataggtgc aatttgttta caagcctttg ataaagaagg aaaaacttct atacttgaat
     6601 ttaatcaaag agtattagga gtgaaactgg aagatttaaa aaagataaaa agaacaatag
     6661 gtgtcgcttc aggagatgaa aaaatagagg caataaaggc atcattaaaa gcgaaattta
     6721 taaatagttt agcaattaat cacagtttag cacttaaatt attagaagat tgtgactaat
     6781 agaaagatag ataaaagtta acaggaggga atgtatatgt caatatcaaa agaaacattg
     6841 cttgaaatgt ataagagaat gaatcaggct agaaaatttg aagagaaagt tagttggttt
     6901 tttgcaagag gtatggttca tggaacaact cacttatctg taggtcaaga agcgtcaagt
     6961 gtggcggctg taatggcttt agaaaaagga gatttagtat cattaactca tagaggacat
     7021 agtcaattta taggaatggg aatagaccta aataaaatga tggctgaact tatgggtaaa
     7081 gaaactggat tttgtaaggg aaaaggaggc tcaatgcata tagcagatat tgagtcaggt
     7141 aacttaggag caaatggagt tgtaggtgga ggtcttacaa tagctccagg agctgcactt
     7201 acacaacagt ataaaaaaac aggcaaaata gttttatgta gttttggtga tggagcatca
     7261 aatgaaggta cttttcatga aggaataaat ttatcttcaa tatggaaatt gccaataata
     7321 ttttactgtg agaataattt atacggtatg tcaacaagca taaaacgtca tatgaatata
     7381 gaaagtattg ccactagagc agcttcttat ggaatagaag gaatatcaat agatggatat
     7441 aacccaatag aagtatacga aacagtacaa aaagctgctg agaagtgtag aagaggagaa
     7501 ggacctgtat taattgaaag tagaacttat agatggttag gtcattcaaa atcagatgca
     7561 aatgtatata gaacaaaaga agaaatagaa tcatggaaag caaaagaccc tattgaattt
     7621 ttaaaaaatt atcttataga aaataatcta tctaatgaag atgaattaga taagattcaa
     7681 gaatttgcaa agcaatcaat agaagatgca gtagagtttg ctcaaaatag cccaaatcca
     7741 aaaatagagt ctttattaga ggatgtatat gcagactaag aatggtaaag gaggaaaata
     7801 aaaatgagta caagggaatt aacatatgca caagcaataa aggaagcaat gtcagaagaa
     7861 atgagaagag atgaaaatgt aatatttatg ggagaagata taggaatata tggaggagca
     7921 tttggtgtat cagttggtat gatagatgaa tttggaccag aaagagtaag ggatactcct
     7981 atatcagaag cagctatagc aggagcggca gcaggtgcag cagcaactgg cttaagacct
     8041 attatggaag taatgtttat ggactttgta acaatttcta tggatgcaat tgtaaatcaa
     8101 gcggcaaaaa tgagatatat gtttggagga aaagcacaag ttccaatggt tgttagatgc
     8161 ccaggaggtt ctggaactgg ttcagcagag caacattcac aaagtttaga agcttggttt
     8221 tgtcatgtac caggagtaaa agtagttgca ccttctacac cagcagatgc taagggattg
     8281 ttgaaagcag ctataagaga taataatcca gtcatatttg ttgagaataa attgttatac
     8341 agaaaaaaag gttttgtgcc agaagatgac tatgtaattg aaattggaaa ggcagatatt
     8401 aaaagagagg gtactgatgt tacagtaatt acttatggaa gaatgttaca aagtgttgaa
     8461 gaagcagcag agactctatc aaaagaaaat ataaatgttg aaattataga tttaagaact
     8521 ttatatccat tagataaaga gactatagta aagagtgtat gtaaaactgg gagagtttta
     8581 atttgccatg aagcagctaa gactggtggg ctaggtggag aaatatcagc actgataaca
     8641 gaaagtgaat catttgatta tttagatgct ccagtaaaaa gaatttgtgg aaaagatgtg
     8701 ccaataccat ataatccaga gttagaaaaa gcagttgtcc caagagttga tgaaatagaa
     8761 gaagcaataa aatctttaat tgttagataa ggagggatag atatggtggc aaataaaata
     8821 aaagccacac cagcagctag aagtcaggca agaaaagaca atataaaatt agataggctg
     8881 attggaagtg gagaaaatgg aaggattcat ttagtagatg tgttaaatta tttaaaagat
     8941 aataaagcaa atactactcc tttagctaga agaatagcag aagatttaaa tatagattta
     9001 gaaactattg taggtactgg atataatgga aagataagaa agtgtgatgt ggaaaaatta
     9061 actgcaaaag aaactattgt atcgactaat acatctaaat ctagtgagaa aaaagagctg
     9121 aaaattgaaa atgaaaattc aagcatgttt aacacagtag aaggaatatt tgaaaagcca
     9181 aatccaatga gagctactgt agcaaaacga atgtcagaaa gttattttag tgctcctgta
     9241 tttacattca atatagaagt agatgctaca gaattgaagg tacttagagc aaagttaatc
     9301 gacacagtaa aagaatcaac tggagtaaaa ttaactatga cagatttaat agtaatggca
     9361 gtatctaaaa tacttccaaa tcatcaagcc ttaaattcag catggacaga tgagggtata
     9421 tttagatata aagatgtaaa tatagctatt gcagttggtt tagatgaagg cttatatgtt
     9481 ccggtagtaa aaaatgcaaa caaaaagtct ttaaaagaaa tagcaaaaga aagcaaagaa
     9541 cttgcggaaa aagtaaagac agggaaactt atgccagcag accaagaagg taatacattt
     9601 acaataagta atgttggaat gtatggtata actacattta cacctataat aaatatgcct
     9661 tcaagtgcaa tcttaggggt aggtgctaca caagataagt ttgttccagt aaatggagag
     9721 gctaaaataa aacctataat gaacttatca ttaacatcag accatagagt aatagatgga
     9781 acagtagctg caaaattctt aaaagattta aaagaattgt tagaaaatcc tttatcaatg
     9841 cttgtatagg ttttgatatt ttgttactga tataaatagt ttgaatagat tatattatta
     9901 taattgtttc agtaaattta aagcttttat atattaagaa ttatttttaa cagtaactat
     9961 atgtgaatat gatttgctta attaaaatat aagaaaataa ataggaggag agcctatgtc
    10021 agtagaagta ataatgccaa aagcaggagt agccatggaa gaaggtacta tagtatcttg
    10081 gctaaagcaa gaaggcgaag aagtaaagat tggagaacct atactagaaa taacaacaga
    10141 taaagttaat atggagatag agtcagaagg agaaggtaca cttgctgtaa tcatacataa
    10201 agaggaaggt gaagtattac cagtatttac agtaataggt gtaatagcag aaaaagggga
    10261 aaatcaagag gaagtaaaag ctaaatattt atctggaaat gtttcaaaag aagatatagt
    10321 tgaagagaat caaaatatag aatttaaaga agaaaaaata aacaaaaaag agtgtaatca
    10381 tgattatgat gtagtggtaa taggaggagg acctggggga tatttatcag cattaaaagc
    10441 agctctttta ggtggaagag ttgctttagt tgaagaaaat atactaggag gtacttgctt
    10501 aaatagaggt tgtatcccta caaagactta tataaaaaca gcagaaatat tagaagaaat
    10561 tgaccaatta tcaaagagag gtgtaaaagt aactgtagat aaagaacagg acattaagaa
    10621 ggccataaaa tataaaaata gagtagttaa aaaacttaca gctggtgttg gcggattatt
    10681 aaaaagtaga gatgtagatg tgtttaatct taaagctagt gttaaagaag agcataaagt
    10741 aattttatca gatggaaaag ttttagatac agaaaatata ataattgcta caggttccaa
    10801 agtaaggata ttacctataa aaggaataga atcaaattta ataataacta gtacagaagc
    10861 attagatttg gaaacagtgc cagaagaatt agtaataatt ggtggtggag tgattggttg
    10921 tgaatttgca gagatattca attcaagagg ttctaaagta actatagtag aaatggaaga
    10981 tagagtaatc ccaagaatgg ataaagagtt aagcgaatct ttaaaatatt ctcttagtaa
    11041 aaaaggtata aatgtattaa ctaaaaagaa agtttctgaa tttaaagaag aaggaaataa
    11101 catattagtg tgcattgaag gtgaagagcc aataaaagca gatttatgtc tatatgctat
    11161 aggaagagaa gctaatcttt ctggcataga ggacttagat attaaaatag ataaaggttc
    11221 aatagttgta aatagtaaaa tggaaacaag tataccaagc atatatgcag taggtgatgt
    11281 tactggaggt gtaatgttag ctcatgctgc ttttaaaatg ggagaggtag cagcttcaaa
    11341 tgctctaggt gtgaataaag aagttgactt aggtgcatta cctagttgtg tttatacaat
    11401 tccagaagta gcatcagttg gtattacaga agaggatgct agaaaaaaat ataatgtaaa
    11461 agttggtaag tttaattttg ctggaaatgg aagagcttta gcttctggtc aagaacaagg
    11521 atatgtgaag gtagtagcag atgctaaata tggagaaatt ttaggtatac atatgtttgg
    11581 atgtggtgta gcagaactta taaatcatgc agcatcattt aaagcattag agatacctac
    11641 tgatgaagca agtgagttga tatttggtca tccatgtact tcagaagctt taatggaagc
    11701 tttggcagat gtaaatggag aatgtttaca cttacctaaa aaataaatct aaacataaat
    11761 tattaattta agtctattat taatattaat ttatatcata aaattttaaa acacttactt
    11821 agccataccc cttattatgc aaataattta tttgcataat aaggggtatt ttttatggaa
    11881 aaatatagaa tatagaatcc ttgtagtttt tgttgtacaa aaaagatagt aatataaaat
    11941 aacattatca ttttagaatg atttcaattg taattaggaa aagtgatttt tttgttttta
    12001 aaaaaagtat tatttgtaaa atataaaaaa aaatttctta atatttaaat aaaagaatag
    12061 cttaatttaa atgttattac tttataaaaa aataataagt acaaaaaaaa caatatatgg
    12121 catataagtt gctatataaa taataaggag ggaacaatta taaatgaggc aaagaataaa
    12181 aaacttaatt gaaaatgaag ataagaaaaa tcctttaaca gatgaagcga tttcaagtag
    12241 gttaaatatt cgaagagaag atgttacctt tttgagaaat gagttaaaaa tagaagattc
    12301 aagacaaaga agaaaagttg ttttaattaa agctataaaa gatattttaa aagaagaaaa
    12361 aactataaat aaaaatgata taacaagaag attaaacagc attgggttta cagtatcaag
    12421 atttacagtc atacaatact taaaagagat tcaagattct ggtgacctaa aacttgataa
    12481 agagagtgat gataaaaaag aagaaataat caataataga gaaaataaag atatagcatt
    12541 tgataaactc ataggtagta gtggtagctt aagtacaatt ataaaactag ccaaagcagc
    12601 aattctttat ccacctcatg gtcttcatac tattattatg gggccaactg gtgttggaaa
    12661 gagtgagtta gcagagtgta tgtataaatt tgcagtagaa tcatccagat tccctaagaa
    12721 tgcaccattt atagttttta atgcagcaga ttattcagag aatccacagc ttttgctatc
    12781 acagttattt ggacacgtta aaggtgcttt tacaggagct gatgagaaca aagaaggatt
    12841 agtatcaaag gcagatggag gaatattgtt tatagatgaa atacatagat tacctcatga
    12901 aggtcaagaa atactatttc aactgattga taaaggtatg gtcagaaagt taggagaaac
    12961 aagactaaca cataaaatag atgtaatgat tatttcagca acttctgagc caatagattc
    13021 tcatttactt aatacattta aaagacgtat acctgtaaca attgaaatac cagaattagt
    13081 tgcaagacca ttaaatgaga ggtttgacat tataaataat ttcttcttaa tagaagctca
    13141 aagaatggga gcaaacatac atataaagtc agatgtatta aaagctctta tgctttacga
    13201 ttgtataggt aatgtaggtc aacttagaag tgatattcaa gtagcttgtg ctaggggact
    13261 attaaaccaa cttacaaaca aactaaaaga agtaaatata agcatatcag atgtacctgg
    13321 atatgtaaaa caaggtctta taaaaatcag aaattgtaga ggaaaaatag aaaattatgt
    13381 agatggggat ttaattatag actcaaatct tcaaggtgag atagaaaaga aaaatgaaga
    13441 tatatatact tttccagatg aaatatacaa acttactgaa agaagacata ttgatttatt
    13501 ggaacaaggt ttagattttg atgttataaa tagaatcatt ggaggagaat tagaaagttg
    13561 cattcaaaaa tacataaaac aagttaaaaa agtttctatt agtaacaatc ttccagatat
    13621 aagtccaatt gttggaaaat atatcgtaga gctaacttat gaagtaatca aaatagcatc
    13681 acaataccta agttcactag aaccgaatct acatttatgt ttagcagttc acctaaaagc
    13741 agcttttgag agattaaaag aaggaaaagt aatatcaaat gctcatttga aagaaataat
    13801 aggaaattat ccactagaat attctgtagc agaaaaggta gctcaatatt taaaggaaag
    13861 atataacata aacttaccaa aagaagaaat aggtataata gcaatatatt ttaaaatgag
    13921 tagtagaaga gatatgtctg aacaaaaaaa gataggtgta cttcttatgg ctcacggaag
    13981 agtagcatct tctgtatgtg aggttacaaa taaactatta ggaattaatc acgcaaggta
    14041 tttagatatg ccatttgaga agaaaccgga agaaatgatt gaagaagcta ctacaataat
    14101 aaaagatatt gatgaaggta gaggtgtgat tatattagta gatatgggtt cactttcata
    14161 ttttggagat attatttctg gaaagactgg aattaatata aggacaatat ctagatttga
    14221 tacattgctt tctatagagg ttatacgaag agcagtttta ccagatttaa caatagacga
    14281 gatagttgat gaagttaaag gtatagagcc acaagtaaaa gaaaaggtta gtaaatgtaa
    14341 taatacaaaa gtagatttta gaaaacctat aattttaaca ttatgtatta ctggattagg
    14401 gggagcttgt aagataaaaa aaatcctaga ggaaaatatt aaaagtataa ctgaaaaggt
    14461 tgatatagaa cctataggta tgattgaata tccaagtctt gatttagaga taaaacaaat
    14521 aagcaatgat aaaaatgtaa tcgccatagt tggaacaatt gaccctcaga tagcaaatat
    14581 cccttttttt agtctagtag acataaatga taaagcaaag ttaaatcaaa taaataacct
    14641 aattgataca aatcaaattg gtgaaacaaa taaaaaatat gaattatcag atttaataca
    14701 taaagaagat atatttgtgg atttacaggt taaatcaaaa gaagaagttc taaaatatat
    14761 gagtaaaaaa cttagagaga aaggttatgt gacaaaaaat ttctataaaa aagtagtgga
    14821 aagagataac ttatttccaa ctgaattatc agatgaaata gcaatacctc acacagatgg
    14881 aatagatgta attaggccag ctataagtat agccatctta aagaagccta ttgtgtggag
    14941 taaaagtaaa gtaaaaataa tacttttatt agctgtagac caaaagtgtt ttaatccatt
    15001 aagcactcta ctctcattta tagaaacaga tgaatttaag aagataaaaa acattaaaga
    15061 tagagatttt attagggaga tgatattaga tggagtcaat gaaaataatt agtagcaatt
    15121 taatttttaa aaatattgaa gtgtctaaca atgaagatgc attaaagttt ttgggacaac
    15181 gtttgtttga cgaacagtat gtaaaagaaa gctacataca agctgttgta gcaagagaaa
    15241 aaaagtatgc tacagggtta ccaacagaaa tatacggagt ggcaatacca catacagata
    15301 tagtacatgt aaatgagcca ggaatagcaa ttggaatact gaataaacct gtaaaattta
    15361 taatgatggg tacagacgat actgaaattg atgtaaaagt tgtatttatg ttagcagtga
    15421 aagagccaca agaacaattg caactattag aaaaacttat gactatattt caagataaga
    15481 atatactaaa taatattgtt gacttaagtg aagaaagtgt gagtgattta ttaaactcta
    15541 aattgaaaaa ttaaacatgt aaagtatatt aaaaatgtaa aattaaacat gcaaagtatg
    15601 ttaaaaaata taaaaagatt attaaagatt tctaactata gatatcttta aaataaaata
    15661 atttcaatgg gggtgactaa tgtggctaaa aagatattag ttgcatgtgg aacaggagta
    15721 tgtacttcaa caattgctat aaacaaatta aagaaagcac ttcaagacat tggtaaattg
    15781 gatatggtta atattacaca atgtaaagta gtagaagttg catcaaaagc acctgattat
    15841 gatttaataa tttgtactac acaagtatct agtagtataa aaacaccagt gataaatgga
    15901 ttaccttttt taactggtgt cggaatggat aaattaatca atgatgtatt agaagagtta
    15961 gaattataat taaaaaaatt aaggaacatg gtctctaaaa tcataagttt tagagactta
    16021 aaattaaata atggagggtc tttatatgga gatattaaat ttcattgttg gtctaggtgc
    16081 acaagtaatg atgccaataa taatatgtat ttttggtttg atattaggta caaaactagg
    16141 aaaatcttta agagctggtt taactgtggg agttgggttt ataggtctta acactattat
    16201 agctttgcta acagataatt taggtccagc tacacaacaa atggttaaga atttagggct
    16261 aagtttatca ataatagatg taggatggcc agcagcatca gctatagctt ttgcatcaac
    16321 agtaggggct ttaattattc caataggtct tgtggtaaat atagttatgc taattaccaa
    16381 tactactcaa actgttgatg tagacatatg ggattactgg cattttgctt ttacaggagc
    16441 tttagtggca ggggctactc aaagtgtaat gtggggagta tttgcagcag tagcaaatat
    16501 ggtaattgta ttggtaatgg cagatttaac tgctccaggt atagaggaat atttaggtat
    16561 gccaggtata tctcttcctc atggatttac acaggcattt gttccaattg ctatagtagt
    16621 aaataaatta ttagacttaa taccaggcat aaataagatt gagataaatg ctgatacatt
    16681 gcagaaaaaa tttggattat ttggagaacc tttaataatg gggtctgtaa taggtgtaat
    16741 aattggtatt gctgcgaaat atgatataaa aggaatatta caattaggtg taactatggg
    16801 agctgtatta atattaattc caaagatggc agcattatta atggaaggat tattaccagt
    16861 atcagaagct gcacaggaat ttattgagaa gagatttaaa aacagaggca agatttatat
    16921 aggcttagat tcagcagtag gaataggtca tcctgttaca ttatcagttg cactagtgtt
    16981 ggttcctttg actatactta tagcagctat attgccagga aataaagttt taccatttgc
    17041 agatttagca gtaattccat ttgcacttgt attaatcgta ccaattacaa agggaaatgt
    17101 gtttagaaca ttaataatag gaataattat aattacttct ggattattaa tcgcaactaa
    17161 cttagctcca ttatttaccc aaatggcttt aaatgctagt tttaaaatgc ctgaaggagc
    17221 aactatgatt tcaagtattt gtgatggtgc gaatccatta agttgggtat ttgtaaaagt
    17281 tatgaactat aaagttattg gaggagtagt ttttggagtt atagctttag gaatggctat
    17341 ttacaatagg aatagaataa taaatgaaaa taaaaagcta tctatagaag aataaggtta
    17401 aaacaatttt attaaggagt gtataggtta tgaaaaaata taaatatgat tctaaaagat
    17461 acttactcag tgttacaata ccaggatttt ttatgatttt aatattgatt tatgcactgt
    17521 ttaataacta tacaaatttt aatttgaata tatatagttt gttaataatt gtctgtactt
    17581 atggtatatt caatactttt atatcatcaa gtaatcctaa aaaaattatt attgatagca
    17641 aatgtataca ttttacctca tttatgacta cacataagta tgaaattaaa gatatagaaa
    17701 agataagaat aagagaattt tataataaaa aaatttatct aaaaattaat gatggaaaac
    17761 ttttcaaagg taggtattgg ataagaacaa atatgtttaa taatagtata gcattatata
    17821 gctatttcat tgagttggaa gagtgcttat atccagattc actaaaattt agaaataaaa
    17881 aatttgagaa taagaacata taaaaataaa aaattgaatt gaagcctagc ttaatctttt
    17941 ttgataagat ataagtttga actttatact aaatttttag aattttcaat caagacaata
    18001 acataatagc taagcaatta gaacacaact aagatgtata aggcatgtat atgatgaatg
    18061 ggaggattta attaatgtta ttagagaaat taagaaaaga agtattacaa gcatctttag
    18121 accttttaaa ctataattta gtgacattaa caggaggaaa tgtaagtggg agagatgaac
    18181 aaactggcta tatagcaatc accccaagtg gaatggatta tagaaatctt acaccaagtg
    18241 atattgttat agtagatgta gatggaaata ttatagatgg aaaatggaaa gcatcagtgg
    18301 atttatcaga ccacttgtat atatataagc atagagaaga cataaatagt ataattcata
    18361 cacattctac atactcaagt tgttttgcta ttttaaatga acctatagaa tgtgcaagta
    18421 caactcttgc aaatgaagta gggggttcag taccagttgc aaaattttcg ccaccaacat
    18481 ctaaaaagat gggaaaatgt gttattgaag caattggtga caaaagagca tgtcttttag
    18541 caaatcatgg cgtaattgca gttggtccat ctgtaggaca tgctcttaca gccgctgtaa
    18601 tgttagaaga ttcagcaaaa gtatattact tagcaaaatc aattggcaca ccagtgctac
    18661 tgcctgatga agaaattcaa agagcgagag atgtattttt taatgtctat ggtcaggata
    18721 aataaattag ataaaatttt aaaagtaata tgaaaatata gatattcaat gatattaagg
    18781 agatttataa tgatataata agtttaatag ttaagaagta aaatatgaaa tgtaatagta
    18841 tataaattca tttaagacta aaaagaataa gataaaataa aaaggagtct gtatttctat
    18901 gtattactta ttaaacaaaa gcacaaatcc ttactttaat ttggcattag aagaatatct
    18961 atttttaaat gataaatata atgatgatat tataatcata tggagaaatg aagaatccat
    19021 ttttataggt aaaaatcaaa atccatatca ggaagtatat catgatgtta ttgaaaaggg
    19081 agaaattcct attttaagaa gaatatctgg aggaggaact gtttatcatg acttaggtaa
    19141 tatcaatatg tcattcatac aaaaagatag gcaacttcat gagatagatt ttttggagca
    19201 tactaagttt atgcaaaaga tgctttcaac cttaggacta gatgtatcta taactgaaag
    19261 aaaagattta tttttaaatg ggaaaaaaat ttcaggctct gcacagtcta taaaaagaaa
    19321 aaattctctt tatcatggaa ctcttcttta cgattcagat ttaaataaat taactaaata
    19381 tttaaattca aataaagcaa ctgaatcaaa tgcaactaaa tctgtttcaa gtaaagttac
    19441 aaatattaag tttttactcg aaaaagatat aaattacttt ttagattatt gtgttgaata
    19501 tttaaaatct aatataaaga atataaaaga acttgaattg gatgaagaag atattaaaag
    19561 tgtatacaat ataggagaag ataaatataa aaagatagag tggacatatg gtaaaacgcc
    19621 gaaatttcaa gtagtgctac catttaataa tgaaagtaaa ataaatatac atgttaacag
    19681 atggaaagta tcaaaatttt ttatttctta tgatgataat gtaattaata tggacaagtt
    19741 gttagatttg aatttcttta aagaagaaat aaaagagagt attttagaaa attatccaca
    19801 ttacatagat ttaatcaatt taatatttta atataaaacc tctaatttga taaaattgtc
    19861 ttatatcaaa ctagaggttt ttaatattct tgcatattaa caaccattac aaaattgtaa
    19921 aattaaaaac aacactattg aaaaatcaat atatattgta ataaatatat gtgtttttca
    19981 ttagcaaata gacattaact atttatacaa ctgaaaattt aatatataat taatcagatg
    20041 gttaaaataa aaatattaaa agttttaata aatgatggtt aaaaataaaa tacgaggtga
    20101 taccatgtat agtgttataa ttgttgcagc gggaagcgga aggagaatga acttagatat
    20161 aaacaaacag ttcataaaat taagagaaaa agaaattatt gctcatacaa ttcaagtttt
    20221 ttatgaaaat ataaatatag atgaaatagt ggtatgtata aaaaaagaag aagaagattt
    20281 ttttaaagaa aatataataa ataagtataa ctttaaaaat ataaaaatag catatggcgg
    20341 aaaagaaaga caggactcaa tatataatgg tttaaaaaaa ttagataaaa attgtgatat
    20401 agtacttatc catgatggag caagaccatt tgtcgaccat agaataatca atgaatctat
    20461 aaaggtagct aaagaaaaaa aggctgtggt tgtaggtgtt ccagtaagtg atactataaa
    20521 gatagtgtca gatggaacag tacaagaaac tccagaaaga aatttgctgt gggcagcaca
    20581 aacccctcaa acctttgaat ataatctcat aatagatgct tatgaacaag catataagaa
    20641 caattattat ggtactgatg attcaatgtt ggtagaaaat ataggtcaaa gtgtcactat
    20701 ggtaatgggt tcttatgaaa acataaaaat aactagtcca gaagatttaa atatagcaga
    20761 acaaatatta aatatggaaa aaagagatga agttaacagt agaaggagaa ttatatgaga
    20821 ataggattag gatatgatgt acacaaatta acagaggata gaaaacttat aatcggtggt
    20881 gtagagatac cacacgataa aggtttgttg ggtcattctg atgctgatgt attgatacat
    20941 gcaataatgg attctatttt aggtgctcta gcattaggag atatagggaa gcatttccca
    21001 gatacagatg aagaatataa gggagcagat agtatgaaat tattagaaca tgtgtataat
    21061 ctaataacaa gtaaaggata taaaattggt aatatagaca gtactataat tgctcaaagc
    21121 cctaagatgg ctccatatat tgagagtatg agaagtaata tatcaaaagt tttaaacaca
    21181 gacattgata atataaacat aaaggcaaca acagaagaag gtcttggttt tacaggagca
    21241 aaacagggaa tagcttcaca aagtatttgt ttattattgt tgacaagtca gaataattaa
    21301 tatattatta gtaataagtt taatggatat caagtaatag gcctatatag aatatctata
    21361 atgtatgaat ttaagtgaaa ttaatataaa tataaaatta gctatgataa agaaatagta
    21421 aaatgatgaa atcttcacag agagaaaacg gtggtgagag ttttcaagag gaagtctttg
    21481 aacctgtctt ttagcttaaa ggtataaaaa tctttacggt aggaaccgtt atattcccaa
    21541 aagagagcta ttcatatagc taactagagt ggtaccgcgg aaattaaacc tttcgtctct
    21601 atacttgtat agagatgaga ggttttttat attttcagga agttaatatt caggaggaat
    21661 ctattaatga aaatgtcaaa aatgtttatg ccgacattaa aagaaatccc agcagatgct
    21721 gagattacaa gccatcaatt aatggttagg tcaggcatga taaaaaaaat gacttcaggt
    21781 gtttataacc aattaccaat ggggttaaga gtgttcaaaa aaattgaaca aataattaga
    21841 gaagaattaa ataaaaaaga ttgtcaagaa atactttgtg cagccttact tccttctgaa
    21901 ttatggaaag agtctggaag gtggactgct atgggtgaag aaatgtttag actaaaagac
    21961 agaacagaaa gagagtactg tcttgggcca acacatgaag aagcattcac tgatataata
    22021 agacaagaaa ttacttcata taaacaattg ccactgaact tgtaccaaat acaagtaaaa
    22081 tatagagacg agagaagacc aagatttggt gttatgagaa ctaagacttt tacaatgaag
    22141 gatgcctata gttttgatgt agatgataaa ggtttagata agtcatatca agatatgttt
    22201 gatgcatatg taagtatatt taatagatgt ggattggaga atagccctgt acaggcagat
    22261 tcaggagcta taggaggttc tacatcagcc gaatttatgg ttaagtcaga agttggtgaa
    22321 gatgaagttg tattttgtag tggatgtgat tatgcagcca atgtagaaag agcagaatct
    22381 tgtaatctag catcacaaaa agaagaaatg aaagaattag aagaggttca tacaccagga
    22441 gcagctacaa taaaagagtt agaagaattt ttaaaaactt ctccagataa gtttgctaaa
    22501 actttagtat atgaggcaga tggaaaaaca gttgtagtcg ttgtaagagg agatagagaa
    22561 gttaatgaaa tcaaagtatc taatgccata ggttctgtta tagaatttgc tcttgctaca
    22621 gatgatgtag tgagaaaagt aactaatgca gaagtaggat ttgcaggtcc aataggtata
    22681 aatgctgact atgtatttat agataaagaa attgttgaac aaagaaacat agttgttggg
    22741 gctaataaaa ctgagtacca tataaaaaat gctaactatg gaagagattt tgaaggaata
    22801 gttggagact ttagaaatgt tcaagaaggc gacaagtgta tagtatgtgg taagccactt
    22861 gaaatagcta gaggtgttga agtaggacat atatttaaac taggaactaa gtactcagaa
    22921 tctatgaatg caaacttcat agacaaagat ggaaaatcaa agcctatagt aatgggatgt
    22981 tatggaatag gtgttgaaag aacagcagca gctataattg aacaacataa tgatgagaaa
    23041 ggtataatat ggcctttgtc agtagctcca taccatgttg tgatagttcc agcaaatatg
    23101 aaaaatgaag aacaaatatc aatagctgaa aatatatata atgatttgca agctatgggt
    23161 gtagaagttt tattagatga cagagatgaa agaataggtg tcaaattcaa tgattcagaa
    23221 ttaataggaa tacctatgag gattacagta ggtaaaaata ttaatgaagg taaagtagaa
    23281 tttaaactta gacataaaga agataaagag ataattgaca tagaagaaat aaatgaaaaa
    23341 gtaaaggcag agtttatcag gaataatgtt aggttaggat aataacttaa ataagatatt
    23401 gtatagataa tcaaagttgt actttataga caccatttta tctatatata aggatataaa
    23461 atggtgtttt tttataaaac aaataaatga ggaggtaatt aaaatggcaa aaaatgaaaa
    23521 acaatttgtt gaagaaataa caaagatgga agatgatttt ccacaatggt acacagatgt
    23581 aataactaag actgacttag ttgattatgc cccagtaaaa ggttttatgg ttgtaaagcc
    23641 atatggatat gcattatggg aaaagatgca agaatttatg gataaaaaat ttaaagaaac
    23701 tggacataaa aactgttatt ttccattatt aataccagag agtttattaa acaaagaagc
    23761 agaacacgtt gaaggttttg ctccagaagt tgcatgggta acacatggag gaaataaaaa
    23821 attagaagaa agattatgtg taagacctac ttcagagact ataatatgta caatgtatgc
    23881 gaaatggtta aaatcatata gagaattacc atatctttat aatcaatggt gctcagttgt
    23941 tagatgggaa aaatcaacta gacctttctt aagaacatct gagtttttat ggcaagaagg
    24001 tcatacgtta catgaaacag cagaagaagc acaagaagaa acaatacaac aattagaagt
    24061 atataaagcg ctatgtgaag aattacttgc aatgccagtt gtagctggtc aaaaaagtga
    24121 aagtgagaaa tttgcaggtg gtgaaagaac atatactata gaagctatga tgcatgatgg
    24181 taaagcatta caatcaggta caagtcactt cctaggtcaa cactttacta aggcttttga
    24241 tattactttt gcagataggg aaggaaattt agctaatcca taccatacat cttggggagc
    24301 atctacaaga cttataggtg gacttataat gactcatagt gataacagag gtttagtatt
    24361 accaccaaga gtagctccaa ttcaagttgt tatagttcct atagctgcta aaaaaggaaa
    24421 tgtaatggag actgttgata aaatatatgc tgatttaaaa gctaagggag tagctgtaga
    24481 agttgatgat agagataact atacaactgg atggaaattt aatgagtggg aaatgaaagg
    24541 tgttccagta agagttgaaa ttggaccaaa agatatagaa aataatcaag ctatggtatt
    24601 tagaagagat actttagaaa aggattctat gcctttagaa ggattagctg atgctatatg
    24661 tgatttattt gatgttatac acaatgatat gtttgaaaaa gcaagaaaac atagagaaga
    24721 caatacatct atagttgaaa atatggatga gtttagaaaa gctttagaag aaaagccagg
    24781 attcataaag actatgtggt gtggagatgc agaatgtgaa gctaagatta aggaagaaac
    24841 aggagcaact ataagatgct taccttttga acaagaaaac ttaggtcaca agtgtgtgta
    24901 ctgtggaaaa gaagcagata gtatggtagt aatggcaaaa gcttactaaa atacatttaa
    24961 ataaaaactg gggtagaaag tatttaatac tttatcccag ttttttgttt tttatgagta
    25021 ataaaatgta tttgtaacta tgtcataaga taagtcatag atacttatat taaaaagaat
    25081 ataatagaga actattttta ataattacat attaatgaaa gcaaaatatt ttgttatata
    25141 gattttcaat aaagcaaaca ataaatataa atgaatatta ataaggagtg cttttaattt
    25201 ttagaaaata aatgacttaa aagaaagatt tatgaaatta aaactaggat tttctgatat
    25261 aataaacatg tgtattttta aataaaaagg atattattat tagatttgat taaaaataaa
    25321 cttgcaaaac aatgtactag tttaatatta tagttatatt gaataaaata tcaggaggaa
    25381 gtaaaaatga atgtaagagt aaggtttgca ccaagtccaa caggatttgt acatataggt
    25441 agtttgagaa ctgctttata taactattta tttgcaaaaa aaatgggcgg agaatatata
    25501 ttaagagtgg aagatacaga ccaaagtaga ttagtagaag gtgctataga gaatatgctt
    25561 aatgctatga aatgggctgg ggtcaaccat agtgaaggtg ttatattaga tgatagtgga
    25621 aaagtagtac aaaaaggaga atatggtcca tatattcaat ctcaaagatt agatatatac
    25681 caagaatata taaagcaact tttagatagt ggaaaagctt attactgttt ctgtacaaaa
    25741 gaaagactgg atgaagtaag agatgctcaa agagcagcag gagaaacagc taaatatgat
    25801 ggtcattgta agaatctttc taaagaagaa gtagaagcga atataaaagc aggaatacca
    25861 tatgttataa gattaagatt gccagagaat catacaatta agtttacaga cttagttaga
    25921 ggagatatgg aatttaatac taatgactta gatgaccaag tattaatgaa aacagatgga
    25981 tttccaactt atcattttgc agtagttgtt gatgactatt tgatgaaaat tacacatgtt
    26041 ataagaggag aagaatgggt atcatctact ccaaaacatg tttatttata tgaagctttt
    26101 ggatgggaag cacctgtatt tgtgcattta ccaaatatac ttaataaaga aaagaaaaaa
    26161 ttaagtaaaa gacaaggtga tgtagctgtt gaagacttta agaaaaaagg atatttacca
    26221 gagggattag taaactatgt agcattagtt ggttggtctc ctgaagataa tcaagaattg
    26281 tttacaatgg aggaattaga aaaagccttc tctgtagaaa gagtatctaa aagtggtgga
    26341 gtatttgata ctgaaaaatt aaattgggtt aatcagcact atataaaaga tggtgatgat
    26401 gcttatctaa cagacttagc aataccattt ttaatagaag atggatttat aacagaagaa
    26461 gaagctacta ataaatatga tttcttaaag agtatgatat cagtacttaa agagaaatta
    26521 caatatgtaa aagaagttac tgaacatgca agtatatttt ttggagataa aatagaggta
    26581 gaaactgaag agggaagaga gttcttaaga ttagaacata tacctacttt aatagatgct
    26641 ttgagagaaa aaatagaaaa aacagaggta ttaaatgctg agtttgttca agctatgctt
    26701 aaagaaatac aaaaagagta caaaataaaa gggaaaaatt tatttatggg ctcaagaata
    26761 attttgacag ggcaaatgca tggtccagat ttaccaaaag taatggaagt tttaggaaaa
    26821 gaaacttgct taaatagaat agcttatgta aaaaataata tattatagtc ataaaagtcc
    26881 gctaatttag aagcggactt tttttgtgtt agtatttggt gaaaatatct gcttaagaaa
    26941 atgaaggtat ttaaggtaat aaatttaata tataaaaagc aaagcaaaaa tataaataaa
    27001 atatgaataa taaactaata aaatattaag catagaagaa gggaatctat tattttagtt
    27061 tgatttaatt attaaggggg taattactat gtatagtaat attgatgatg ttaaaaagga
    27121 attaaaggag ctatgcttag aatatgttac aattttagaa aaattaaaag atgaaaaaat
    27181 gattacagaa gaaacttttg aaaagtgctc ttctcaaaaa aaaatatttt tagaagaaca
    27241 atagaggatg gtaatagtat tattattatg tagtttgaaa gatagtacga aaaatgatag
    27301 taatactatt atttattgca attttcaact ataatataat atataatgtt aagaagataa
    27361 tttataaaaa tacagtgagg tgatgagtgt gaaagtttat aatacattga ctagaacaaa
    27421 agaagaattt gttccattag aagaaggaaa ggtaaaaatg tatgtgtgtg gtccaacagt
    27481 ttataactat atacacatag gtaatgcaag accatttata atatttgata ctctaagaag
    27541 gtatttagag tatagaggat atgatgtaac ttatgtacaa aactttacag atgttgatga
    27601 taagattata aatagaagtc atgaggaggg aatatctcct gaagaagttg cggctaagta
    27661 tataaaagaa tattttgtag actgtgatgg gctaggaata aaaagagcta ctgttcatcc
    27721 tcaggttaca gataatatac aacagattat agagtttata aaagagttgg aagataaggg
    27781 atatgcatat gcagtaaatg gtgatgtata ttttgataca aataagtttg aaggttatgg
    27841 aaaactttca ggtcaaaaac aagaagattt agaggcaggg gctagaattg aggttaatga
    27901 ccaaaaaaga catcctatgg actttgttct ttggaaggca aaaaaagaag gtgagccagg
    27961 atgggatagc ccttggggag aaggtagacc aggatggcat atagaatgtt ctgttatgtc
    28021 taagagatat ttaggtgaaa caattgatat acatgcaggt gggcaagatt taacattccc
    28081 acatcatgaa aatgaaatag ctcaaagtga agctagaagt ggaaaaacat tctcaaaata
    28141 ctggatgcat aatggatata taaatataaa tgatgaaaag atgagtaaat caaaaggtaa
    28201 tttctttaca gtaagagata tatcaaaatt atatgattta gagatagtaa gatttttcat
    28261 gttatcagca cattatagaa atccagtaaa ctttagtgat gagatgttaa atcaagctaa
    28321 agcaggtctt gaaagactat ataatactaa agaaaaatta gaatttacgc taagtaattt
    28381 agtagaatct cctttaacag aaaaagaagt agagcttgtt aaagaattag atgactttag
    28441 acaaaaattc atagatgcta tggatgatga tgttaataca gcagacgcag taagtgtaat
    28501 atttgaatta gctaagttaa taaattctaa tgtagatgaa aattcttctt tggagtttgc
    28561 taaaaaatgc ttagatgaat ttaatgaact tacaggagta ttaaatattg taaataagaa
    28621 aaaggatact gtattagata aagacattga agaattgata caaaaaagaa ctgatgcaaa
    28681 gaaaaataaa gagtttcaat tagctgatga tattagacaa caattactag atatgggaat
    28741 tgtattagaa gacacaagac aaggtgttaa gtggaaaagg atataaaaat ggaaaaaact
    28801 gaattagtta ctatgtcacc attagtttta gcatacttag gtgatacagt gtatgagaca
    28861 tatataagag aatatttaat tagacagaat acacaaagaa aagttaatga tttgcataaa
    28921 ctagctataa agtatgtaaa agctaaagct caggcaacta taattcatga agttgaaatt
    28981 gagttaactg aagaagagag taaaatttat aaaaggggaa gaaatcaaaa gtcaaatact
    29041 tctcctaaga atgcagatat tatagactac aaacatgcaa ctgggtttga ggcattagtt
    29101 ggatatttgt atttaaataa tgaaatagag agacttcaat atattatcaa taaaggaatc
    29161 aaaattattg aaagagatat gtaaatatta aagagtgttg tcttaataat aagtaataag
    29221 gcaacacttt ttttataact aatgtataat aaatatatag aaattagtaa tgagaaagga
    29281 taaaaagtta tgaaagtaaa attaatatca catacacctg aaccagaaaa agtaatagct
    29341 atggcagcta agctatgtta ttcaccagtt gggacagacg aaatagaaaa agacttaaca
    29401 gatgaaagta tagagaaatt tttaaatatg ttattaagta taggtcatgg gtcaatatta
    29461 gaacatgctt catttacatt ttcaatagaa ggtatttcaa gggcatgttc acatcaaatt
    29521 gttagacatc gtatagctag tttttcacaa caaagtcaaa gatatgttaa attagaacaa
    29581 tttgagtaca taattcctcc agaaatagaa aaaatagaaa aggcaaaaga gttatttata
    29641 gactccatga aaaaagacca agagaattat gataagttag ttgaaatatt gtttgaaaat
    29701 cattataatg acctaataaa aaacggtaaa aatgagaaaa cagctaaaag acaagctgag
    29761 aagaaagcaa tagaagatgc tagatacgta tttccaaatg catgtgaaac aaagatggta
    29821 tttactataa atgctagaag tttgtttaat ttctttgaac atagatgctg tgaaagagca
    29881 cagtgggaaa taagaaattt agctgtggag atgttaagag aggttaaaaa ggttgctcct
    29941 atattattta aaaagactgg tccaagttgt gtaaatggaa gttgtccaga aggaactatg
    30001 acttgtggag atatagttca agttagagaa aaatttaagg ccttgtagga ggtgaataat
    30061 ttggcgagta tagaaggaag aaatccggta atagaagcaa taaaaagcga cagagaaata
    30121 gataaaatat taattgcaaa ttcagctaaa gaaggttcaa ttaagaaaat aataggaatg
    30181 gctaaagata agaatataat tattcagtat gttgataaac ataagttaga tgaagtaagt
    30241 acaagtcatt cacatcaagg tgtaatagct tatgcaagtg agtataagta ttatgaatta
    30301 gatgagctaa tagatttagc aaagaataaa gatgaagacc catttttcat tatattagat
    30361 gagataacag acccacataa tttgggttca ataataagga cagcagatgc agtaggagca
    30421 catggagtta tcattccaaa aagaaggtct gtacacataa ctcctgtagt tgccaaagca
    30481 tctgctggag cagtagaata tatgccagtt tgcaaggtaa ccaatatagt taatacaata
    30541 aaaagactca aagaagaagg gctgtggatt gctgcagctg atatggatgg agaaactttt
    30601 tataaacaaa atcttactgg tcctcttggt gtagtaattg gaagtgaagg ttttggaata
    30661 tctagattgg ttaaacaaaa ctgtgacttt atagtaaaaa tgccaatgat aggtaatgtt
    30721 acatctttaa atgcatctgt agcaggtgga atacttttat atgagatatt cagacaaaga
    30781 ctggataaaa gtaaataggc atgaaaaaaa atataaatca ttacttaata atagatggct
    30841 acaatattat aaatgcgtgg gacaatttga aagaactggc taaagaggac ttagaagatt
    30901 ctcgagaaaa gcttattgat gacattatag aattttctga gtttatgggt tataagacta
    30961 taatagtttt tgatgcttat aatgttaaaa attctcgaga aaaagttgaa aaaagaaaac
    31021 acataacaat agtttataca agagaacacc aaacagcaga tagttatata gagaaattta
    31081 taacttcttt atcaaagtat gatgatgtaa aagtagccac taatgactat gcagaacagc
    31141 aaatgatttt aggaaaaggt gctactagaa tgtctgcaag agaattaaag ttagagttag
    31201 atcgttccaa gaataaaatg aaggaaaaaa atatcggttt gaggaaaaaa attcagcgaa
    31261 attggttgga ggaaaggtta gataaagaaa cattgtcgaa acttgagaac attcgtagaa
    31321 agcgttgaaa ttactaggtt ctttagacta taatatacct ataaatttgt tttatatatg
    31381 ggggagattt acatgttggt agcaaaagaa aaaagttatg agttagtgga caattgtcag
    31441 caggatgagt ataacattgt attaagagca agtgaagggg ataaaatagc actagagtac
    31501 attattacta aatacagaaa ttttgtaaag gcaaaagcaa agtcatattt tttaattgga
    31561 gcagataaag aagatataat acaagaagga atgataggcc tttataaggc agttagagat
    31621 tttgatggaa gtaagaccaa ttcatttaaa tgttttgcgg agatatgtat aacaagacaa
    31681 ataataactg ctataaaaac tgcaacaaga caaaaacata tacctttaaa ttcatatgtt
    31741 tctttgaata aaccaatata tgatgaagag tcagacagaa cacttttaga tattatagct
    31801 acaagtatag taacagatcc agaagagctt ataattagta aagaagagtt aaaaaatata
    31861 gaatcaaaaa tgaacgaatt gttaagtgac ttggaattag aagtactaga attatattta
    31921 aatggaaagt cataccagtt tatagcggac aagcttaaaa gagatgtaaa gtcaatagat
    31981 aacgctttac aaagggttaa gagaaaatta gaaaaacatc ttgaaaacag aaatgattaa
    32041 tagtaaaata cttaccataa gcttattgtg agcaaaatta atttatatcg aaagataagg
    32101 gaggaaattc aaaatggcta aagctaaata cgaaagaaca aaacctcatg ttaatatagg
    32161 gacaatagga cacgtagacc acggtaaaac tacattaaca gcagcaataa caaaaacatt
    32221 atatgacaga tatcaattag gagaagcagt agatttcgca aacatagata aagctccaga
    32281 agaaagagaa agaggaatca caatatcaac agcacacgtt gagtatgaaa caccaaatag
    32341 acactatgca cacgttgact gcccaggaca tgctgactac gttaagaaca tgataacagg
    32401 agcagcacaa atggacggag caatattagt ttgttcagca acagatggac caatgccaca
    32461 aacaagagag catatactat tatcaagaca agttggagta ccatatatag tagtattctt
    32521 aaacaaatgt gacatggtag atgatgaaga gttattagag ttagtagaga tggaagtaag
    32581 agatttatta acagaatatg atttcccagg agatgacact ccaatagtaa gaggttcagc
    32641 attaat
//
LOCUS       DAYAOY010000043        32586 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYAOY010000043 DAYAOY010000000
VERSION     DAYAOY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.043"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(182..1537)
                     /locus_tag="ACGI6B_002050"
     CDS             complement(182..1537)
                     /locus_tag="ACGI6B_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9751493.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
     gene            complement(3444..3947)
                     /locus_tag="ACGI6B_002051"
     CDS             complement(3444..3947)
                     /locus_tag="ACGI6B_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFG9751494.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
     gene            complement(4164..5573)
                     /locus_tag="ACGI6B_002052"
     CDS             complement(4164..5573)
                     /locus_tag="ACGI6B_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFG9751495.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(5577..6707)
                     /locus_tag="ACGI6B_002053"
     CDS             complement(5577..6707)
                     /locus_tag="ACGI6B_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9751496.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(6719..7522)
                     /locus_tag="ACGI6B_002054"
     CDS             complement(6719..7522)
                     /locus_tag="ACGI6B_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751497.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(7904..8785)
                     /gene="pepI"
                     /locus_tag="ACGI6B_002055"
     CDS             complement(7904..8785)
                     /gene="pepI"
                     /locus_tag="ACGI6B_002055"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HFG9751498.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            9241..10215
                     /locus_tag="ACGI6B_002056"
     CDS             9241..10215
                     /locus_tag="ACGI6B_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFG9751499.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(10331..11392)
                     /locus_tag="ACGI6B_002057"
     CDS             complement(10331..11392)
                     /locus_tag="ACGI6B_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFG9751500.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(12004..12855)
                     /locus_tag="ACGI6B_002058"
     CDS             complement(12004..12855)
                     /locus_tag="ACGI6B_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9751501.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(12872..13792)
                     /locus_tag="ACGI6B_002059"
     CDS             complement(12872..13792)
                     /locus_tag="ACGI6B_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFG9751502.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(13902..14816)
                     /gene="murQ"
                     /locus_tag="ACGI6B_002060"
     CDS             complement(13902..14816)
                     /gene="murQ"
                     /locus_tag="ACGI6B_002060"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFG9751503.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(14915..16012)
                     /locus_tag="ACGI6B_002061"
     CDS             complement(14915..16012)
                     /locus_tag="ACGI6B_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFG9751504.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(16245..17591)
                     /locus_tag="ACGI6B_002062"
     CDS             complement(16245..17591)
                     /locus_tag="ACGI6B_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFG9751505.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(17680..17988)
                     /locus_tag="ACGI6B_002063"
     CDS             complement(17680..17988)
                     /locus_tag="ACGI6B_002063"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9751506.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(18251..18649)
                     /locus_tag="ACGI6B_002064"
     CDS             complement(18251..18649)
                     /locus_tag="ACGI6B_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751507.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(18651..19802)
                     /gene="anmK"
                     /locus_tag="ACGI6B_002065"
     CDS             complement(18651..19802)
                     /gene="anmK"
                     /locus_tag="ACGI6B_002065"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HFG9751508.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(20242..21261)
                     /locus_tag="ACGI6B_002066"
     CDS             complement(20242..21261)
                     /locus_tag="ACGI6B_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9751509.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(21255..21935)
                     /locus_tag="ACGI6B_002067"
     CDS             complement(21255..21935)
                     /locus_tag="ACGI6B_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751510.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(22087..23994)
                     /locus_tag="ACGI6B_002068"
     CDS             complement(22087..23994)
                     /locus_tag="ACGI6B_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9751511.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(23995..24747)
                     /locus_tag="ACGI6B_002069"
     CDS             complement(23995..24747)
                     /locus_tag="ACGI6B_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751512.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(25063..26217)
                     /locus_tag="ACGI6B_002070"
     CDS             complement(25063..26217)
                     /locus_tag="ACGI6B_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9751513.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(26220..26687)
                     /locus_tag="ACGI6B_002071"
     CDS             complement(26220..26687)
                     /locus_tag="ACGI6B_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9751514.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(26701..27189)
                     /locus_tag="ACGI6B_002072"
     CDS             complement(26701..27189)
                     /locus_tag="ACGI6B_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751515.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(27203..28534)
                     /locus_tag="ACGI6B_002073"
     CDS             complement(27203..28534)
                     /locus_tag="ACGI6B_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9751516.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(28548..30113)
                     /locus_tag="ACGI6B_002074"
     CDS             complement(28548..30113)
                     /locus_tag="ACGI6B_002074"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFG9751517.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(30283..31068)
                     /locus_tag="ACGI6B_002075"
     CDS             complement(30283..31068)
                     /locus_tag="ACGI6B_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9751518.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(31235..32434)
                     /locus_tag="ACGI6B_002076"
     CDS             complement(31235..32434)
                     /locus_tag="ACGI6B_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFG9751519.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT        10918 a         5600 c         3278 g        12790 t
ORIGIN      
        1 ctagtatttt tatttgttat ttttatttaa taatcatact ctaacaaata ttataattat
       61 atatatcata atattgtatt tagaagtaaa attttatttt tattattaat tagttataaa
      121 tcaatctaat acaacataat tacagtagat taacctataa aacataataa ttgcttactg
      181 attatttatc gtcctcaaca agtctactta atgtcttatc agataagaat aataatatcg
      241 ctgcaacaaa tagtattact ggaattatag tatatacttt aatcatatca aatttaagag
      301 taaatgcata tatatatcca tatcctttac cagctataaa agtagcaaat gtccatactc
      361 ccataagaac acctagaagc ttcttaggtg catacttact tacaaaagag ttaccaagtg
      421 gtgagaataa catttcacca agacttaaaa gaataccaaa catgattatc catagtatac
      481 tagctttagc agtttcagga gctccaactc ctcttgaaaa ttctgcacca acaagcatta
      541 agaatgcaag acctaagaat attaatccaa gacctgtctt tttaaataaa ctcatatctc
      601 cttgtggtct actagcaagt ttaaaccata atgctccaag aactggtcct aatactatac
      661 atacaagtga atttaatgag tcaaaccaag caagaggaac atcaaatcct ccaacattca
      721 tgtttacaaa tgctgcacca taatcataaa ctgcaagata agttagatac cagaataccc
      781 agaaaattac agagaagaat gatataagta taatagccca aattctcttc ttatccatgc
      841 ttgtaagagg agacttatct ttttcttcaa ttttttcagc aactacacct tctttaaaag
      901 gtctctttcc tgcattccca aggaatctcc atccatatat gaaccatatt gcacctatta
      961 cacaaagtac agctgctata aagaaacatt gagaaaatcc aagtacacca ttttttgcaa
     1021 atgttttaag gtaaagaata ccaactgcta tagtaccaat aaaagaacct atattaacaa
     1081 aagaatattg tactgaaaat gctgtatcaa gttcttcttg actatcaaaa agctgtccat
     1141 ttaccgcaga tacgttacct ttaaagaatg cagtaccaat tgaaactaag attatcattg
     1201 catttaccat agaaactgta gttgcaatag aaccaagata gtatcctacc cccatgataa
     1261 gcatacctat aggtatagta tatctagctc ctatccatct atctgatata taccccccaa
     1321 ttataggtgc aaggtatgtg aatgcaacaa ggttggcttg cattatagca gcttgtcctt
     1381 tatctattcc aagaccacca tgaactatag ctgttgtaag gaatatatat attaagtact
     1441 ttgaagcata atatgctgct ctttcaaatg agaaagttgt tgagcagata taaaatccca
     1501 taggaaattt acccttcttt tttactgttg agctcataat tttaaatcct ttcttaattg
     1561 tatttttatt ttttacgttt tgtccaaatt ctttctttta tgaaagatat aatattatta
     1621 ttttgtaaaa caatttgtaa ttattattaa gataattgcg catttatttg tagagtacat
     1681 attctcatgg aataaaatta atacacttct taatcataaa tttaaattat ataaattcac
     1741 tattttcaga tatagttata aattaatata ttctcatcta ataccatgaa aaaaccttcg
     1801 ccatatgcaa ttcttatttt ttacgtgata gtattttaca aactaaaatc ccccttttgt
     1861 agcaacttat gtaattacat tttagtgctt ttttaaagga gtctcgaata aagcaacata
     1921 tattttgttc aaataatgtt tttatttatt ttttaaattt atatattact tcacccaacc
     1981 aattctcaac ttaaataact aaactactat ctattcacag attaggctta tttcttacac
     2041 attagtttat aataaacact gtgaatctaa acataaaaac tgttatttta aagtaattac
     2101 ccatatattc gctttgatac tatttattga ggctatactc tttatgtata atatattatt
     2161 tataaaatac ctcaaaatat attattcata aaatacctca aactgaaaac tttttcacga
     2221 atatttattt ttcacctccc cttttttatt tcaccatgta aatcgtacca taaatatgca
     2281 cttatatgca agaaaaaaat ctattaatcg acagtaaata attagaaaaa ttcaaattat
     2341 atttatcaat tatattatat aaaatatttc ctatgaaaat ataaattaaa ttaatgttca
     2401 gaatagtttt tagatatgaa atcttgattt ttttattact ttacttattt gctcaaatat
     2461 atttttgtat gcagtatata ataatcaaat tatacacttc ttataactgc attgatttga
     2521 aaataatatt aataattaat agaataaaaa atttaaatta aatttaagaa aatttgttaa
     2581 gttgattgaa tactttttta tgtatttaat taattttata aattcaatta ttttctaaat
     2641 ttaggtatgc tttactatat aaaaatgatt ttttatctta atcttctgaa tataagtgaa
     2701 tgctatcatt ttaagtatct tataagcaat gtaactatat atctctaata actacttttt
     2761 taacatgctt aaactattta taagctatta tatttatgta ttaacctaat aactttttca
     2821 ctaaaaaata acaatctatt caaagataaa ctatagtact tcttctactt tgtttcaata
     2881 aaatactaaa taaatttcgt acatccacaa tttttcaggc tacactataa atatacaata
     2941 tttatcttag ttataagttt aagaatattt ttagtcttat tttgtcgaat atctttttaa
     3001 taaaacttca taattctaag catattttta cactgtgttt tcatgtacaa aaataatgct
     3061 tacaaataaa caacataaat aaaacttaaa taagatggat tctattcaac atagtatcca
     3121 tcttattcat tcaaaaccta aaaggatttt ttataatatt atatatttta aaatctaagt
     3181 aactcattat agtatcaatt tcacttaaag cttacaatac acttattaat cttgttttat
     3241 attaaaaaca tctacaataa caatttaaat ctttaagtga tatccatctc acacctaaag
     3301 gagtaggctc tccgcactat tctgtaattc ttcaatacaa tcattattat tttttatata
     3361 atttaattat atattttatt ttaccaaaac cagcatacat aaataatact taaaatagat
     3421 taaataaagg atactaaaac actttattga ggtatatgac ttgaaatata tgaactaact
     3481 tcattaggag aaatatctaa aatagttgca aattcttcat ttaataacct ttcagcttct
     3541 ttcattatat tatcatctgc ttgatgtgtt tttttaccaa tagattttac atactctctg
     3601 ttattatcta tacttcttac taattttatt aactcttcac atttaccacc tctgagtatt
     3661 tttttaaatt gctcacttct caatttttca ttatctatcc atgatgtatc catattagga
     3721 atatcattta ttaatgaaag cacattaatt ttagaaagta actttctcat tggtactttt
     3781 tcattatcta caggaatttt tattactgta ttattagaat aaataggact caatacataa
     3841 tactcttttt ttattccatt tgtaaatcgt tcatttgtta tatccatgac tttgcagact
     3901 cctgtcatac catacattat ataatcatca atcttaaaca aaaacatcac catccttaat
     3961 aaaaaaaata gttttgacaa ctaaatccac cttgccaaaa ctatctgcta tatattatat
     4021 tttaacacat tttgagactt aaatgtaagt aaaacttata ttattaaaat taaaaaatac
     4081 attaaccatt gattaatgta ttttttagat ttttataaca agcatatcaa aataaattaa
     4141 tacagcttat ctcctgtgaa tacctataaa attggtgctt ggctattcat tgagtaccca
     4201 tcatttctta catcatttat ttctaatatt tcatcatttg ttttaacctt agtaagtttt
     4261 ttaactgctc cataaggttc ttttaatgaa attttatctc ctcttccaga aataacccat
     4321 aaaattttgt accctctagg tattacttgt agtttatttt cacctttacc atcagtaaag
     4381 tagactaaca aattaatctt tttattatta gcatactcaa agactggaga aaatctagta
     4441 cctccaccta tcctaattct atcttttata tctttttcag ttctcacttt atatacacgt
     4501 ctaatttcat tatcacattc tataattgta atttcatgat tatagttttt cactatacta
     4561 agaacttctt taatagcttg tttaaactct tcatcactaa tacttccact tacatcaagt
     4621 gcaactgcta tttctgactt atgacttcta agttctcccc tcaaatctaa acgatttggt
     4681 tgtcttctat ttcttcttgt tatagtcttc tttttattat tttcaactgt tcccattatt
     4741 ctattaagat ataaattcca tggcaattca cccttactat ttttaagtga tgatatcata
     4801 ctttctaaat aagcaggaac tttacctttt tgagaactgt taataaattt ttcagtaaat
     4861 tctctaaggg tattttcatc tatctcattg gattcatccc atatatcatg agttctttcg
     4921 gggctataat caatttctat atctccatat ttatcactat catcttcctc accatcttca
     4981 tccacttcca tcaaatctaa ttcagtctga atcttttcta cataatattc aaaaggttca
     5041 tatggttcaa gttttaagga atattttaaa tttacccact ctaaggtagt tgcatatggt
     5101 ggtaaattgt ttagaaattt atttactact atatccattg ccatattaat tgctaatgta
     5161 ctatactttc cttttaactc ctttgctctc atcaaatgca tagataatat atgatgaatt
     5221 tcatgtttaa ttgtagtctc catttgcttt atgtcaagcg ttaaaaagat tattggatta
     5281 aaatatataa tatatttagc tcccttaaaa ttcacacctg taggactact tatatcaaat
     5341 cttatttctc ttgacatctg aaacaaaaaa tatccataaa aattatcttc ttcttccata
     5401 agacttaaat tcactttatc tacaagacta aaaaactcat acttaaaatc ttgtggtata
     5461 tctatatcaa atttctcatt agcacgattt gcctttagca tagaatgagt atttataatt
     5521 tcacttgctt tattataaag ctcttttgct tgtttatcaa aataattttc catacattat
     5581 ccccttatta aactataaga ttcaaaatat gattttacaa actcttcatt ttctatagct
     5641 tttttatata cttcaatata actgtttcta atatccttca taatacctat cattaaatct
     5701 acaggataca tttttaaaaa ttcaataaat ctattaatat agtggtttga ttcatattta
     5761 tcattcttaa tatttaattc taagtctttt agaatgttca tagctgatag ataaagtctt
     5821 gtatgactct cattttttac tctttctaca atagactcat caatagattc tcctaaaaaa
     5881 acatcttcat aggatattaa tggttcagag tttgattcaa taaaacttat aaattcttct
     5941 gcaataactt tacctacatt accctttaca acatttaaaa atactgctct aggaatagaa
     6001 ttattttttt ctttataaac tttataaatt ttagatactc tctcataact tctaggagtg
     6061 gctcttacat catcttcatt tattttatgc agatattctg gaaaagtgga aataaactcg
     6121 ataacttttc tttcaatacc ctcatctatt gcccatttta tccattgtgt atggtcactc
     6181 tccatattta accaaacaaa cctattttct tgggctgcat ccatatctac cacttgataa
     6241 tcaaaatctg aaccatattt acttgatgga ttcattgctg ctaaaatctt tacatcatca
     6301 tgcagtttat atccattaat ttctctattt aaaattaaat tcataagttc ttgctgtact
     6361 gtatgctcac aacgatttat ctcatctata aataaaagaa ctgtcttact tttagatatc
     6421 tcttcatcaa tttctcttaa tttgttatga acagcataca ctgttgtttt cttttcagtc
     6481 ttatatccat tagaattaac tcctacataa gattctatag ttggaagacc acctatttca
     6541 ccctctttaa gtaagtttcc atcaataaca attaaactcc aatcattttc tttggcaagc
     6601 ttatttgcta aagctgtttt tcctattcca ctttcaccta ctattaaagg tacttctcca
     6661 gtggataata ttaagtcaac gctttttaat gtgtctataa aattcatttt tctctctcct
     6721 acataatctt taatttctca tctacagcta ttttttttgc tgtcttactt ccatatttat
     6781 aaataaacat aattttatct attggtatac tttcgctatt tctatctata acactacaat
     6841 tcaaaaaatc tctaatatac ttttcctcaa catcttcttt ggataatact tcttttaatg
     6901 cactctctaa ttcatcttta gatacttttt tcagaatgta cttaatcact aaaatattca
     6961 cttttaaaaa ttccaacatt ttatttttat ctaaatcagt tataaagcta atagctgctt
     7021 cattattttt tatagctata agttctgcat tataactagg tgaatctata tatctcaagg
     7081 catcataact tattctcact gcctcctctt gtacactttc ataaggttgt tttataaact
     7141 ttatcgaatc ataatttttt cttactgcta acagttgtaa ttcttcactt ggatttttag
     7201 catatttaat tgcccatcct gcttgattta tagccatttc aataacttta tcagttgggt
     7261 ttttgatata atccagtata ctccatccct catttactgc ttttatcatc atatcttctg
     7321 ttggattatc tataaattta attgctctaa tattattatc tattgctagt tcttgcatct
     7381 ctctggttgg attatttata tattctagta caagcccatt ttctttaata gctaacaatt
     7441 tcatttcatc agttggatta tctattgtag ctataatatt gggattattt tttatcattt
     7501 caataactct caatttttcc ataatgtgcc tttccttcta taattatttt tatatctata
     7561 ttaattataa taatctattc ctatttatat aattgtttct tattataatt aatatacttt
     7621 ttaatcaaat ttaaacagaa taattatacc aatatataaa aaaatttcta aaaaacaata
     7681 aaattaagtt ataaacacca atatatttct attatttcaa taagtataca attagattag
     7741 aatctaacgc tagtaataaa aaattacttt cccaaaatag atataagcct cattttatcg
     7801 ttcaaataat gaaaatatat ttgatttcat gcaaaaagag tgcctaatga actaaaaagt
     7861 tcattttgag acactcattt ttttatataa atttatatag tttttaatcg ttcgcattca
     7921 accactcagt taagactttt atatatttct cattttcttc aacgaatggc atatgtcttg
     7981 aatactcaaa taattcccac ttactattag gtattctatc atacatagtc tttgcaatat
     8041 atggtgaaca taaatctatt gctccactag ttactagaca aggctctttt atttcatgta
     8101 atctatctgt aaattcatat ccagaaagag ttcctgtagg agaaaattca ttttgtcccc
     8161 atcctacaat atatgcttca gaacctgatt ttttaggtct tctcaggcac tctggagaat
     8221 cttctgtcac ttcacctgca caatacattt ccataaatct ttctaatgca tcattatatt
     8281 ctttgctaga gtaatctcca gtatttactg catcaagtaa tgctttttgg tcaacctcac
     8341 tcatatatga tattcttctt ttttgttctt tctcccaaag ttttgcagaa gaaagagtag
     8401 atgaaaggat gtaagactta ataccttttg gtttatattc aatagcatac caaatagcct
     8461 gcatacctcc ccaagattga cctaacagat gaatttcatc aagtcctaga tgttttctta
     8521 gttcaataag ctcctctatc caagtatctg cattaaatag ctcaggatgc ccttctacaa
     8581 atgaatttcc acaaccaatt tggtcataca ttattacttg tctaccactt tcagctatct
     8641 tatccaatac ctcaaaatag ttgtgtgtag aacctggacc tccatgaagt aatactaatg
     8701 gcttcttccc ttccgtattt tctcctacta tacggtaata cgttttaaaa cctttaaaag
     8761 gcatatatcc ttcagtaatt ttcatgttta tctcctccaa tttgtaatta taaacaataa
     8821 tcaagttcat taacaatact atttaaaacc tcttatacat atttgattat gtattttaat
     8881 ctttatacct acttttataa atagtataac ataaaagtta aaaatctcta tactaaacca
     8941 attataaaat tatattaact atataatttt attttttaat aatagtatat acatatttga
     9001 ttattttcat ttgctattta aaatcaattt taagttttct tcttataata aacttcctct
     9061 ttgtggtctc atgttagtct ctaaatttaa aattttacaa aaaattttca taaaataaaa
     9121 ttatattgat tgtatctaaa caaatagggc aataaaataa ttaagtatag ttacttacct
     9181 ttcaagcaat attagtttta tgttagaatt ttattatatt gatttacagg aggtttgcat
     9241 ttgaataata ttaattcact taaagtaata aaattatctc ttggatgttc tcttgctata
     9301 tttattgctt ggctattaaa attagaatat tcaatggttg ctggagttat agtactactt
     9361 acagtaaagg acactaaaag agaaactctt aaaggctcca ttggaaagat ttatggtttt
     9421 cttttatgta ctattttttc atatttatgt tttaatattt taggatataa tttgacttca
     9481 ttttcaatat ttatatttat aattatacca ctttgttttt tgttaaatat tcaagatgtt
     9541 attgctatgt gtgtagtcat agcatcccat tactaccttc aaggagaaac ttctatcaaa
     9601 tggattttaa atgaagctgg aatatttgca gtaggaactg gaataggtgt cttaataaat
     9661 atgtatattc caacaaatat acataaaatc catgaagttc aaaaaaagtt acaggaagaa
     9721 gtgagtattg tacttataga cattgcagat attattgtaa atcctaaaaa agaaaatgga
     9781 tatagtagag atttatatac attaaattca cttattgaca gcagtatttc tgaaacttac
     9841 gataatatta ataatacttt attaagtgat actagattct tcttagagca tatggatatt
     9901 ataaaaagtc aacgggatat cttagaaaat ttgtatagtt atgtatctca actaaattct
     9961 acaccaccac aagctcatat tctatctgct tttatacata agataggtta tactgaattt
    10021 gaagctgaaa caggcaatct tcttcttgaa gaattaaaga gactaatgct tagtatgaaa
    10081 aaccagcctc tacctgttga cagaacagag tttgaaaata gagctatttt attcctatgc
    10141 ttgactgagt taaaacagtt tttagttaat cgtaaacatg ctcagatgct tagagataat
    10201 aatttttata aataaaaagg ggagaaatct agcagttgac ctgactacaa aatatcagat
    10261 ataatattaa atgcgcccct agtaagaggg cgcaatgttt ataacttatt ctactgtcat
    10321 gattaaatca ttattcatat cgaacataca ttatttgacc aagtccaata ttagcttttt
    10381 catcagtgat ttctatttta atagtcacat catttttttt acagtacttc gctaattctg
    10441 caccagaacg atttttacca ctaaaatcta aagaaaatgg cttatcatat ttaaaaggat
    10501 attcaacagg tttaccctct ggtgctaatg caaacatccc atatggtgtg ttcataaatc
    10561 ctattacagc aatcgtaaga tttgatgggt ctaaatttga ttcactacct gtaaatgtaa
    10621 ttgtatttac tgatgaatta actacactac atcgaacacc ttcatacatt acatgttttt
    10681 catttatatc ttctcctttt ttcccaaaat ttccaacagc ctttgcccct tcttgatgtg
    10741 gtaacatagt gtgcttccca ggtggtgctg gtttctgtgt ctgttctggt tttaccgtct
    10801 gtactattgc accaactttt ggatatgctg tcttatcaaa tggtaatggg tggctaagac
    10861 tcttgtatat gatatccttt cctgctgatt tattagcatc aatatctaca tgatacaaac
    10921 gcccatcacc aaaacggata agattccact ggtggtcatt tgatgtgctg tgtacataat
    10981 aagattcaat tcccatagca tcacacaaag ctttcatgct cctagccatt cctgagcatg
    11041 cacctttttt agtaaccaat gtataggcag tatttgctcc ctctactgca ttccagtcaa
    11101 catatgttgt gttattttta atgtgattgt aggcaactaa aatcttagca tctgtggtca
    11161 tattctttgt gattttcttg ttcataaaat ctgctgttgc ttgtgctact gagtttagtt
    11221 gggcttgtga cagctttgga ccatatacgc tattggttcc agtgacatag ctttttggtg
    11281 tttttgctgt tacctctttg gctgtcatac cataaactgt catactcata cctgagatta
    11341 aaactattgt tgtaatcaat aacttggtag ctaacttcct aattttcttc atacttaata
    11401 ttctccttta taaaacattg ttacttccta ttctctacca ttaataaaat taatttagga
    11461 tttaaaaagg tggattgtca acatgtatat ttctccattc atacattcgt gaaatattta
    11521 aatttcatgg aaaatctaat agatattttc tagataattt gtatacaata caactcgcaa
    11581 atcagggtgt ttgctgttag tttgagaaaa tgatgatgat taatacattc ggaatctttg
    11641 acctataata aataccattg tcttcaatca ttaataatgg taagagtaaa gagaattatc
    11701 aataatacag ttaaaatcaa ctttgtatac atttcataca ttctctccca acaacattat
    11761 acttcttata atttgtatat tcaatacgtt ttctgggaaa atataggata tatctataaa
    11821 aatataaaaa aggagttgaa cttaaaattt gattaaaaaa aatcaatttt aagacaactc
    11881 atttgtatta taaaaatgct atatattatt attaagtaag tcaatatttt agatatttat
    11941 ctatttctta ttgacttacg cttatagtct aacttatttg tttagttgat atataaccta
    12001 gttttaagca ttattaatat ttaaataatc aagtatcctt tttgtttctt gtagtttctg
    12061 ttctgcctca tttaagtttt cttgaattat agaaatatat attatatcta tcaacgtaag
    12121 cattgctatt cttgaagaaa tagctccttc tctaagcctt ttttctatat ttggtactac
    12181 tagttttaaa tcactaatct tactaagatg attgtcacta tatttagtta tacaaatgac
    12241 cttacatcct tttaattttg cattttctat agactttatt acttcttttg attctccaga
    12301 gtaagatatt gctatagcta tatcttcttt atctacattt gaagatacca taagttgtgt
    12361 atgactatct aaagaggtaa atgcctgttt atttatcctt aaaagtttca tctgtaaatc
    12421 tagtgcaact aaagccgaac cacctactcc aaatatatat acatttttag cctttattat
    12481 ctgttcaact gcttctttta ttatatcttt ttcattcaac aaccttgttt ggttgatagt
    12541 ctctatgttt ttgtttgtaa ttttgtttat aacactatct atattacaat catagttttc
    12601 atctacatat gtgtattcat tttctttgcc catcaaatct ttagctatct caatttttaa
    12661 atccccaaaa cccgaatatc ccatttttct agaaaaccta actatacttg ctgtacttgt
    12721 ttttgagttt gacgccaatt ctgatacaga aagcttactt atagcctctg tattattaag
    12781 tatgtaatca gctatttttt tatctacttc cgttaaatct tcatatatct gttttaattt
    12841 taaaatgcaa ctcatactct atcacctcta cctatttact ataatatttc ttcttagcaa
    12901 gtaaacatgc tcctaaatct ggagtcaaca ctggtctcac tataccaaat ctcgttttat
    12961 tcaaatattt tttaagtggc tctaatatta aatctcctga cttaaacact ccacctgaat
    13021 aagatacctt tatctttgta ccttgttcaa aatgatgctc taatcctatt ataagcctac
    13081 ttaattcata agctgcatca tcaaatattt tactagcagt attatcacct tcaataactg
    13141 cttttaaaca tatcgtagca aactttgcta tttctattct atctcctttt atttcatcat
    13201 ttacatattt tattatgtca taatcattct ctatattaag atgtcttttt attaaatcat
    13261 ataattttgt ttttgaacac cttccatcag actgcattgt gaacattttt aaggtagcca
    13321 ttcctatata atacgctgaa ccttcatctc ctaatacata gccccatcct ccacatctgt
    13381 gaagattccc atctttatct aaagcctgtg ctattgaacc agtacctgca attatattta
    13441 tacctttttc cccatttaaa gaacctgcta atgctatttc tacatcatta cctagagtat
    13501 aatttatatg actatatgca ttttttgttg ctacttctaa tgcatacaat acttctttta
    13561 tttttccata tccagcaagt ccaacaaatg catacgttat ttcttctaca tttatcttag
    13621 catctttaca aacttcttcc aacccagtta ttaataattt cgttagatta tcaaatccta
    13681 tttgattata atgacaagtt cctttggtta tggttcctac tatatttaat tcttcatcta
    13741 ctaatgtaaa tgttgttttc gttcctccac catctacacc taaaaaatac atactatcct
    13801 cctacaatat ctttaataat tctttcaata cttaatatct aaaaataacc ttccaatgct
    13861 gtttttttaa tattgaaagg ttatcagtac tattatttat tttacaattt ttctattgat
    13921 tttaaagctt cacttatata accttcttgt ctatctagga tactctttgc actatcctta
    13981 tctaaaccac tttttatcat aaatatagcc aatttaacgc tattatctgc ttcttctaag
    14041 aatttttctg caacatcata ttttacattt gtagctatca ttattattct ttttgctctt
    14101 tcaaccaact tttcatttga tgtcttaaca tctaccatca agtttgagta tacttttccc
    14161 atcttaacca tagaagctgt tgttaacata ttacatacca ttttttgagc tgtaccactt
    14221 ttcattctag ttgaacccat tattacttct gcccctacta taatgtttat tggtaaatcc
    14281 gcttttttac tcatttcact attcggattc atagttattg atagtactgc acaccccaat
    14341 tccttagcat actccattgc acctataaca taaggagtcc ttcctgatgc agctatacca
    14401 catataacat ccttagatgt aactcctcta tctattatat cttgctttcc aagttcttta
    14461 tcatcttcag caccttctat tgctttaaat attgcttgat ttccaccagc tattatagct
    14521 tgaacaagct caaaatccac tccgtaagtt ggaggacatt ctgtggcatc tagtattcct
    14581 atcctcccac tggtacctgc tcctatatag aataatctac ctccctgttt cattctatct
    14641 actattatat taacagcttt tgctatactc tccttttgtt cttcaacagc ataggctaca
    14701 gttttatctt cgttatttat taattctatt atgcctaatg tatcttcttt atctatttgc
    14761 aatgtattct tatttctacc ttctgtaact aaattttcta aagtttttat taacatatca
    14821 tttcctcatt ttcttaatat taaattttta aacacatttt aattccaact ttcataatta
    14881 tatttttata ataaattaat attttcataa taaattattt tgttgagggt ttaaaattaa
    14941 acttttgcca tggtttaata taatctaata taaataattc ttcttttctt atatatccta
    15001 ctacattcga tttaccactg tttttcatat cagtcaaggc tatttgtaac tcccctgcat
    15061 aatgaccata ttcactactt tcaattacta tatctcctct ttttattatt ttaggagtat
    15121 taaatagttc aaatttatat cctttatatt taacacgaga ttgagtagac cttatcagac
    15181 tatcagatat gtcccctcta ttaaaatgta tttcttcaaa gagtatcttc ttctcaactt
    15241 ctcgtatatt ttcctctatt gctacatcaa atgtaaccat atcttttctc atagaaccta
    15301 atacctttag ctcctcatat gttggataac aatttgatat tattatatca tttatattat
    15361 tcatagctat tatatgcttt acttgaatat ctataggtag atttctgtgc atctcaattg
    15421 ttggaagccc atcagttaca ggccatggtc caaatgtagt ttcattttga cttgttacaa
    15481 aaactgctgt tcttaatcca tacttattaa atctttctgt acattttata aagtgttcta
    15541 aatttaatcc actatatcta tgtggataaa agttatgaca tcctatcaaa ttttctttat
    15601 ttggttggta gtccattatt gtatctatat aattagtatt attactcata ttgatttcta
    15661 ttttaagatt ttgaggatta aaagtcatta atgcctcttc agaacctgta aatcccatat
    15721 caagccttat tccgtctgcc ttcgtctttt taaaaaaatt caaatctttg tagcttattt
    15781 ctaattcacc aaatactctg ggatttacat caagtattac ctcaaaacct ttaaacttag
    15841 cgtattcatt tatactttta aattttttaa ttatattttc tttagtatct ttaactgata
    15901 ataaacatga aaatatacgt gtaaatccat tttcagcagc tttatctata taatcatata
    15961 tttccttaga tgttgttttt tctggataaa ttgatattcc taatcttccc atctgtgtca
    16021 tctccttata tcctatatgt gtttaaattt atttcgaatt acacgaaact tttttaaaaa
    16081 tttgatgaaa aaaagtttca tatatattta taaaaaaaga aaagtttacc ttttcttttt
    16141 ttataaacaa tcaacaaaat atcttcgtta cttatttcat aactgttata ctttgaaatt
    16201 tatactaatc taatatagtt tttaatttcc aatatagttt ctaattaata agcttcttgc
    16261 tcttcaactt tttcttcatg aagcaacctc ttatcgtaca tcttgacaaa tggatagtaa
    16321 attattattg atatagctat acataataag ttaagaactg aagctctcca atctccacca
    16381 gtggctaaat atgcccctac tggtcctggt aatgtccaag gagcagtaac tactactctg
    16441 gatactaaat tagcactcat agcaaaccat gaaactgttg ccattactat tggtgctaca
    16501 acaaatggta ttattaatat tgggtttaat actattggcg ctccaaatat tattggttca
    16561 tttatattga atattgctgg tattacagtt gcttttccta aagactttcc ataagctgat
    16621 tttgctctaa atgcaagaag taaagctaag cctattgtag ctccagaacc tccaatccat
    16681 ataaaccatt gaaaaaaggg ttcagcagca atacctggaa ctgcttgacc cgcagcatat
    16741 gaagctgtat tagcatctaa taattgtatc cataaaggtc ttgccaatga acctattatt
    16801 gatacaccat gtattccaaa tgaccaaaaa aacgttatta aaaatattag tataattact
    16861 gatggtaatg tgtcagtggc tgaaactaat ggagttacta atttagctat gaagccatgc
    16921 caatcaaacc ctaggaaata agtaacaata gccattaaaa ttattattaa tgcggctggt
    16981 gtcagagctt caaaagacct tgtgactgat gctggaactg cttctggcat ggttatcttg
    17041 aatttagatt tgtcagtaaa tctataaatc tcaacaccta ttatagatgt tacaatagca
    17101 acaaacattg aacctccacc aagattagcc attggcattt gccatccttg aggaattgaa
    17161 ttataccaag ctaaaagttc tggactcttt tcaactatct ttaataaatc aggggtaaga
    17221 ccctctattg cagtaggaac tattgtcaaa aggaatgtag ctgtagctaa taaacctcct
    17281 gtcaatccat ctaacttata tgactttgcc aaactataac ctatgccaaa tgtagaatat
    17341 actgatataa taaaaaatgt cattctatat ggtagtaata ttgtaactgc attttccttt
    17401 agatactgag ttacccccca tgaatctggt aatggaggaa atgctaatat caagaaaaat
    17461 gacccaacta ttataagtgg taatgaagct acgattccat ctcttacagc tcttaagtga
    17521 cgttgttctg ccaatctggc catagaagtt gataatttag tgtctaaaaa ttctgtaaat
    17581 ttatctagca tcttttctct ccttaattaa tatttatttt tattatcatt aaaattttta
    17641 taaaattaac tttcttctaa tctaacaaca ctgcaactac tatttactca acagctcttg
    17701 aacttgtttt aataactttg gtcctcctaa tggactataa gcttgtgctt gtattaatgc
    17761 acatggtaca ctaacctcat cagcaaaagc ttttaaatag tcaaatctat gtctaacttg
    17821 tggagctacc atagcaatat cccatccatt tttaacttca tcctcaaact cttgagtacc
    17881 aacagccaaa acatctatat ctattccatt ttttgctcct tctttttgta atgcattaac
    17941 tgctatagca cttgacatcc ctgatgaaca aacaaataat actctcatat tgattccccc
    18001 tgttttttta tttcttttca atcattatag catgtatttt tgattacagg aatacgtttg
    18061 tatatatttt tttcatattt tacattttaa attgtaattt tatttcatac atttatatct
    18121 tgctttaatt gctttattta tctaatcatt cttttgtcta aaattacaca ctacttaaaa
    18181 tactcatatc ttttcttaaa aaagaaaaaa gactatctta ttattaacat aaaatagtct
    18241 caatactttt ttaaatatgt attcctttat atatccagaa gtaacctatt attgcaaatg
    18301 ataaagcaaa taaaatatac agtctaaaaa atcccaaaga aaaacctctc ttagtttcaa
    18361 caaagtatct tgtatatgca actgcaaaag atgtaattaa tgaatttcct ataacaactc
    18421 caaatctatt atctattcca aatatagata ataacaaagg aaaaactaca gtacttacta
    18481 gagatgaaac tattaatatt aatccacttt taaaacttat agttggaata ttataatctg
    18541 ttctttgatt tttctccatt tgtttcttag tatttttatt actttttgta ctttttgcat
    18601 ttttataatt attatttttc tttcttttct tttttttatt attagacatg tcatttacct
    18661 ccaaaaggtt taggtgttat attccctaaa ataactttat ttttagcccc tgttgcagat
    18721 attacattag aaaaagaatt atttaatgtt tcattgccca agattacaaa tgccaaagcc
    18781 tcctttgcat cagaagaata cccatattca tcttgagtta atacttcaac ctctaataat
    18841 aactctttta tatatcctat taaagttaga ttatgagctc ctccacctcc aattaataaa
    18901 gtatctacat taacctttgg aagtatgaag tttttataat tataatatat agcttttgcc
    18961 gtaaacatag ttactgttgc tatcaaatca tatttatcta catgtttata tttgttaagc
    19021 atgttatcca aataaacttg tccaaatacc tctcttccag tgcttttagg tggcgcttga
    19081 ctaagatact tatgactcat caaatctttt aacatttctt cattaatttt accttttgat
    19141 gcaaagtatc catttttatc atactttcta ttaaataatc tttgacatac tccatctatt
    19201 atcatatttc ctggtcctgt atcaaatgca aatacatcat ctatgttaca tgatttaggt
    19261 attatagtaa tatttcctat tccaccaata ttttgaagtg ctaaattttt attgttatca
    19321 ctatataata aaaattcact atatggtact aatggagcac cttctccacc tgctgctata
    19381 tccattactc taaaattaga tattaccttt gtatttgttt catatgctat aactgctggt
    19441 tcacctattt gaagcgttga agatacaaaa ttattataac ttcttggtat atgaaagatt
    19501 gtttgaccat gagatgcgat aaaatccaaa tttgctatat gaaagtttgc tttatgacac
    19561 actgatttta cagctttact aaataaatat ccaagcttaa aatttaagct acagattaaa
    19621 tcaacactcg attcctcttc tatacagcac tttcttattt cgtcttttat atctttagga
    19681 atttctagag ttataaattc tatttgctta actttagtat ttctaccatt cccactaatt
    19741 tcagctagta ctgcatcaac tccatctaag gaagttcctg acattaaacc tacactatac
    19801 atagttatca tccttttaag tttatagtta ttcctcccca aatcaaggta ttaagtttaa
    19861 taattttaga attaatctta tttcatcttt aattacgata atttactctt atgaaattaa
    19921 ttgagttaat cttatacacg ttttttacct tagctaaatc ttacattaat ttattttcag
    19981 tgtcaatgta ataccatgta attttttttc acaaacaaaa catattctct ctccaaaaac
    20041 catattaaaa tagttactac aaataaaaaa cataccaggt acctatctga tatgtttttc
    20101 atatataata attattattt tataatatag atagagcatc atttaaggca ctaattaaat
    20161 cttcactatt ttcaagaact aaatttattc ttattgtata tgtcgatttg tatatgatga
    20221 tagattttat agtctaataa cctaactatc taggcttaaa taatccgata tatcataaaa
    20281 atagatatta aaaattgtat atgaaccatt ttccgaacat acctcaatct catgccctaa
    20341 actatctatc attttcttta caaaatacat ccccattcca gtagacttat acaattgatt
    20401 tcttccattt ttccccgtaa atcccttgtc aaaaacccta gatatatctt cctcatttat
    20461 acctatacca aaatctttta tagataatac cactccattt tctaactttt ttgaattaaa
    20521 ttcaatctta ccaacttcct ttgaatactt tatagcatta ttaataattt ggtctaaaac
    20581 atatgacatg catttagaat ctgtgtaaac atcattttct aattcattca aactaacttc
    20641 tacattattt tttataagca aaaatgaatt attttttata gacattttta tcaatttttc
    20701 taaattaaat ttatttataa atacatcttc aaacatagtg ttgcttctac tactatacaa
    20761 aatagagttt actaaaaaat ttatctttgc aacctgattc ttaatatcca aactatcttc
    20821 tatgctactt aacctatctg taattatact taaagaagat attggaattt ttatttcatg
    20881 aacccattta gctatgtact catccaattc ttttaatgat tgtttataag tctctatgtc
    20941 actattattt tttattttta agttttctat aatatctttt attagatttt cttcaaaaga
    21001 ttgtccatca actaaataac tgtctatttc accactattt tctatacaat tatataaatt
    21061 tgtgtaacta tttctaaaat ttatataatc tataacaaaa aataccatta ttataaacag
    21121 tactaaaaaa tctaaatata ttaactctga atatttattt ttaaaaatat ctatagcaaa
    21181 taagtagata tttattatac taactacaac taaaggtaga gcaatatact tagacctacc
    21241 tttcaaatat tttactagca ttgtattata tatcctaccc ccttcttagt ttttataaaa
    21301 tcatcaaaac ctagttcttt aactttactt ctcaatctat tcatatttac agtaagtgca
    21361 ttgtctgtca caaactcatc attatcccat agagtcatca taagactttc ccttgataca
    21421 accctattct tattttttaa taataatgtt aaaattttta tttcgttttt agtcaactct
    21481 atacttttat ccttatactt atgttttata atccctttcc ccacatcaaa catcaaatct
    21541 tcacaataca aaatactatt agaatcacta taattataga ctcttcttat tataccattt
    21601 attttggtaa ccaatatttc tatagaaaat ggtttggtaa tgtaatcatc tgctccatta
    21661 tttataccca ttattaaatc catatcagaa tctcttgatg ataaaaatat taatggtaca
    21721 tttgaaatag ctcttatttt ttcgcaccag taaaatccat tatataatgg taaatttaca
    21781 tctaaaagta ctacatctgg ttttttatct ataaattcat ctaatactac ctcaaaatca
    21841 tcaattaatt ctactttaaa tccccatttt tcaagtgcta atgcaatctc ttttgataaa
    21901 tcaatatcgt cctctactaa taaaattcta tacatttgta tattctccat tatcctaata
    21961 tttcactatg ctatgaatta ttaatatcat aacatatttt atatgaattt attacccaaa
    22021 ttatttagtt aatcaacaag gagactctta ttacaagaat ctcctttctc tttattaata
    22081 tatcaactaa tttttataca ctacactctt aaacttatta acagatactg ctccatatat
    22141 tatgaatatt atcaaacata tcactaaact tagtatatat ataccaaata aatcggtcat
    22201 caataaatct cctgctaatc tcaatgcaaa ataactagat atagttgcac ttaacattgg
    22261 caaagtataa aacatagcta cttctttaag tatagctcta tttatatctt tttctgtagt
    22321 acctattttt aacaaaatct tatatttctc tttatcttca tatgcatcac tatatatttt
    22381 catatatatt atacttccag ctacaattgc aaagactaag aataaaaaca caagcactac
    22441 atatgcaaac ttcatccatt gactctcacc ttgagcttta tatccatttt gaatataccc
    22501 attttttaaa cttaaattct tttcaatatt gttgaacatt acttgactat tttcttcttg
    22561 aaataccttt gcaccataaa aatacacttg ttgaccctta tctcttagtt taaaatatgt
    22621 atcatcattt accactataa tactttgatt aagtcctgtc cctagtactg gaacttttac
    22681 ttctcccctt gaaatattat ataactgacc atcaagtttt atcttggctt cttttgtagc
    22741 tattgatgct atcgtttgag ggctttttaa ctctattaca ttatttccac taactaatct
    22801 ttcaccatac tcttttaatt ctttatcatt gccattcact ttaagagtat tacaaaaatc
    22861 actatatttc attatagtat ctgttgtatc ataagcttct tttatatctg ttttctttaa
    22921 aattgttagt tctggattta ctgagtactt tatcttatta gtatctttta ttatattctc
    22981 tatttgtttt ttattcactt catctttatc cattgaaact atgctaatag tgtacacatt
    23041 tctttgttct tgggcattct tatgtatacg cttcatcgca actgaagcac caagagttga
    23101 tattgttgta gttataatta tagctattat agcataagtt ttatagtttt tatttaatcg
    23161 gtagtttata ttgtttattg caattatatt atttccatta aataatattt ttttattttt
    23221 gattataata tcaaatataa caggcataac tgatttgaat aatccataag tacctactat
    23281 tattatacct ataacaggta gtacactagt tatatctcca gttcttatat tggtagaaag
    23341 atagtagcca tatcctatta ctataattga tactatagct aatatatata tacctattcc
    23401 tacctttggt attttttctt gttttttaga tatattgatt agatttatta cactacttct
    23461 acatattgtt ataaaacctt ttacagccat tattataaat atagaaataa agattaaagt
    23521 agcatatagt atagcttcta tactaagtcc attagatacc tttatattaa atccagataa
    23581 ctttattatc actacttgaa aaaatctaga tgttattatc cctataatta ttcctgttat
    23641 acaagaactt attcctataa gcatattttc tacaaaatat atttttccta tagtatataa
    23701 atctaatccc ataaaagaca atattccaat ttctttagac ctattcttaa aaaatatatt
    23761 tgttgcatac catataaaga aaaataaaaa tactgatagt atcattatta cagctactaa
    23821 cactgatagt gtaaattttt tattttcatc ttgtaacaca tttatagcat caccatatct
    23881 taataattca aaattagtta gcacaactac tgaaaaaacc atagatataa aaaatgctag
    23941 ataagttctt atgttactct taaaattatt ataagcaatt ccaagtatac tcacttacat
    24001 ctcacctccc atagaggcta gcatatccat aatttttcca taaaattctt ttctactatt
    24061 tcctttgttt aatttacact ttatatttcc atcatttatc atatatactt catttgaata
    24121 acttgctgtt agagggtcat gtgtaatcat tataatagta gcgttatttt ctctgtttat
    24181 tttttctaat gattctaaaa gttcatatgc tgatttagag tctaaagcac cagttggctc
    24241 atcagcaaat atgactgttg gatttgtaat taatgaccta gctgctgcgc ctctttgttt
    24301 ttgtcctcct gataactgat atggatattt atctaaatga ttttctaatc caaatttttt
    24361 agctatagaa aataccttag cttctatctc cttacttttc ttttttccta atgctagagg
    24421 aagtgctata ttgtccatta gtttcatatt gtctaataga ttatagtctt gaaatataaa
    24481 ccctattttt tctcttctta attttgatag ttcattattt tttacttttg atatattaat
    24541 tccatctagt attatttctc ctgaagtttg tttgtcaagt gtagacatta aatttagcaa
    24601 tgttgtcttt cctgcaccag atggacccat tattgatata aaatcacctt cattgactgt
    24661 aagagatata ttttttaaca catttgtttt aaatcctttt attccataat cttttgatat
    24721 attttttatt tttaatattt ctttcataat aatagcctcc agttgctaat tttttaatat
    24781 atatttattt taaaggtttt atataaataa atccttaagt aaatattaca ttttaactgt
    24841 tagcattaca cttttgtaac atatggaaga ctattataat tatagtagaa atatcttaaa
    24901 ataaaagcat gatttacttc tttgtataaa caaagaaaag aagctcctaa gaagcctcta
    24961 atctatacaa ttattcttct ctataaaaaa ccaaaataca cattgattgt ggcaaataca
    25021 gatactataa acaagtgata ttactactat atattaaaac aattattcct gaagataatt
    25081 caataagact atcaagcaat aagctaagca tccaaaaaga cttaatacca ttaatgtata
    25141 attataccca atattaatgc tcaaaaaacc agcaacagtt ggaataatca gagatgaacc
    25201 tcccataaat atacttaaag ctattaattg gctactactt ctaagttttt taggagaaac
    25261 ttcaaatatt aatctttttg aagtaattaa aattaaagga gtagaaaaca cttgaaaaac
    25321 acacagtata ataatgatat ttgaatcatt tgttaaagca aataatagaa actgaactgt
    25381 tatcataata gcacttaatt ttaataagtt taaactgcta tattttttta ataaataaga
    25441 accagccaga tatgttggta tctctaacaa tgactgtaaa gaccacttta aagatatctg
    25501 agaatttgta gcatttaaag ctatcatttt atctattaca gtacagttat ttgctaccac
    25561 cattgagtat aataagaata aaatacatac tagtaagata tattttttat cttttaataa
    25621 ttcaataata tctttattag ttacatttat tttcttatta atcttttcaa tatcaggtaa
    25681 taatagtata tttataataa gtaatactac aattaaacca atcgctacag ataatccaaa
    25741 atatgagaat ctaagaatca agtaagatgc taccatactt ccaatagccc atccaatcga
    25801 cccaaaagtc ttaataaatg acaaataatt attaatatta tctccacaac ctattatcca
    25861 tgtatcatat aaactacagc aagtatttaa taatcctcct gataaagcaa caagtaataa
    25921 gtatattata aaagttgcag attcagtaga aaacataata actgatgcaa ttccataaac
    25981 tgataaacta attaaaacaa tttttttcat tgtttgatac ttatcagcta atactccaaa
    26041 taataattga aacaatatat tggttattgc atagctcgat aatataattc ctctttcaaa
    26101 cgctgagtaa cctaattttg ataagaaagg tgtgaatttt gttgaagcaa aggcaaaaat
    26161 aatatatgag aaaaaataga atatgataaa tttaaattta atcgctttat taatcataat
    26221 caatcaaaat tgataacttt aataccacta tttccttcct gatttaagtt tgaaagatga
    26281 tattttaccg tattaggatt ctctacaata actaaaggaa tatataaagt attttcaagt
    26341 aacttattat caatttctac taacttttcc cccatattaa cttcttgacc ttgtttaact
    26401 tctaatttaa agttttcttt ttttgttgtt gcactatcca atccaatatg aataagaatt
    26461 tgaacatcat tttgcaattt aattcctata gcatggttag tttgagcaat aaaacttatt
    26521 actcctttag tcggtgattt aagaatattg tcctttgcta tcatagcaaa tccatctccc
    26581 attaccttag ttgaaaatac tttatcagga atttctccaa gattaattat ttttccatca
    26641 atgtatgaat taattgtaat tgcttctttt tttcttttaa agaacattat agacactcct
    26701 ctacataatt aactttattc taattatcat tacaaataac gatattaagc atactgcaat
    26761 tgaggaaaaa agagccttat agctctttcc ccattgtagt tctttaatac tgcttatgat
    26821 ttgtttaatt ccaacaaatt tataaatata ggtaaacatg ataacaatag ttattgtgta
    26881 aatttggaat atctgcccta ataagactaa atagaaagca atcatgtttt cttttatctc
    26941 tttattcttt tttatattgt aaataacata tgcactgtat actgcactaa agttagagat
    27001 taaatttaaa cttgcattaa catcattgtt taaaagttgt gaaaatgaaa tatttgtaaa
    27061 taatgatgtc aattctaaat acactatata taaaacaggt aatattagaa aacaaaccat
    27121 aaccactgaa agaaatatat tttcatcttt taatactgct gtgatatttt ttattttatt
    27181 tgttatcatg tttttgttct ccttatttaa aagttggcca gaaatcctta ttagccacaa
    27241 tcatttcatc taagatttct tttgctactt ttgcacttgg aacagtttta ctcatagtaa
    27301 acgactgcca taatttttga taactttttt ctatccatgc ttctacagct aatttttcag
    27361 aagcaacttg ttgttccatt aaaccttttt ggaaagttgg aattttacct atagacatag
    27421 gttcaaccat atcttttcca acaaggcaag gaatttcaac cattgcatca tctgcaaaat
    27481 tttcaatagc tccattatta ggaacaatca gtaaaaatct ttcctgagta ttaaatgcta
    27541 atgcacaagc taattcaaca ataaattgag aatgttcact tgcatcaaac catgtatctc
    27601 ttgctgtgcc attttttata attctttcac attcttcgaa tgtatccttt tctcttctat
    27661 ccataatttc atttgctctt gtataatttg gattagcatg ttcaacagtt tcatcagcca
    27721 ttaaataata ttttaaataa gtattaggta atgtagtcgg ttctatttcc aataaatcct
    27781 ttgctgctct catagtttct aaccaactag attctttgtg ttgaccttga ggtgtaaatt
    27841 cctcatatcc ttttttagca caatattcct gtaatttagg cattaaatca tttccttctt
    27901 tgtccttaat acttgtccac cacccgaaat gattcaatcc ataatatcta acatcaaagt
    27961 ctttacgaga atttaaacct aaaactctag caaatatttc ctctaaacat actggcatat
    28021 cacaaatatt aataattctt gaatttggtc ttaatacacg acatgcttct gcaactatag
    28081 ctgctggatt tgaataatta agcatccaag catttggaga atatttttcc atataatcaa
    28141 tcatttcgat tattggacct attgttctta atccataggc aattccacct gccccacaag
    28201 tttcttgacc aacaacccca tattttagtg gaattttttc atcttgttca cgtaaagcat
    28261 ataaaccaac acgaatttgt gccattacaa aatcaatatc agtaaatgct tcttctggat
    28321 ttgttgtata actaaattta atttctggat ttttctctct aacaattatt gcacatgctt
    28381 cagcaacttt tctttgtctc tcatcattat tatcatataa tttaattgat tcaataggaa
    28441 atttagcaac actttcaatt aacattgtta caattccagc agtataagta ctacccccac
    28501 cagcaattag tatcctctgt ctttttcttt ccatctttcc ttctccttta taataaattt
    28561 tcaaattctt ctcttacact tggtactgaa agcccaataa taatctgaat agcttttcca
    28621 ttatgcacta aaccatgtgc acctactgcc ttaaaatctt cttctttaga aataattgag
    28681 tcatctttca aggttaatct caacctcgta gcacagtttg taacatctac aacattttct
    28741 gctccaccaa ctaattttat gtattcagat gcttgttgag aggctttact caatttattt
    28801 cctttttcat ctttagtttc taagtattca tttttagtat atagttttgt ttcttcacta
    28861 tcttcctctc taccaggagt tttcatatca aacttcttaa ttaagaaagt aaatacaaag
    28921 aaccagataa atgaaaatat aatccctaca acaacttgaa caacatatac cttccagtga
    28981 tacattccaa gaggaagcca atttaataca acccagttaa taattcctcc accaaaatca
    29041 ccaacaactc ctagggcaaa cgaaatcgtt tgcaagcatg catctaaaaa tgcatgtact
    29101 aggaataaaa gtggtgctac aaataaaaat gtaaattcta acggctcagt aattccagtt
    29161 aatactgcag taagagtagc aggaatcatt aaacctaata cttttttctt tttttctggc
    29221 tttgcagtta catagaaagc accaaataca cctaatgaac caaacacctt cgataatccg
    29281 gataatgaaa atccgattgg atacatttcc tttagagtct tagctgaagt ttgaaaatcc
    29341 tgaatatgag tagcccaata tactgatgta ccacctggaa caacaacact atcataacaa
    29401 attggtgcat atacaaaatg atgtaatcca gttggtatta aaatcttttg taagaatgca
    29461 taacaccaaa ctcctatatt tccactattt ataaaaaacg tttgcaaatg tctaataaca
    29521 ttttgaatat gaggccaaat aaaacaagtt aataatgcaa ctggtatcat aacaaagaaa
    29581 caaattgcaa ctacaagtga tgagcctcta aataatccaa taaaatctgg cagttcttta
    29641 tcataaaatc tattatgaat ataaatgaca atacctgata ttaacaaagc accaaccata
    29701 ccagtatcta atgttttaat accagcaatg cttacaagtc cacttccagc acttatttca
    29761 gctgcgaaat caactccaaa gctttttccc caaaaactta acatagaacc tacaaaataa
    29821 ttaaaagtca aatacgttgc taaagcctcc atacaagctc ttgcttgttg cttttttgca
    29881 agtgctattg gtaaagaaat agcaaataat aatggcaatt gattaaatac catccaagct
    29941 ccactagata ttacacccca aaattggtac caaatatttt ctggtgtagc aagtgaaccc
    30001 ataattgact gattttgaaa tacaattgag aatccaacca ttatcccagc aaaagcaaat
    30061 aatagtgttg gtgtaaacat tgcccctcca aaacgctgta ttttcttcat catataaatt
    30121 actcccctcc tcatattttt agtttatctt tggccttaga tatctattat tttaacaggt
    30181 taataaatat atacaatctt tagggtacat tcggagctta ttcacagtca aataagtttt
    30241 tgcacatttt tatgttcaaa ttctcataaa aaaaaagaat gactacaatt tgcaatcaat
    30301 cccttctgta atcattctat gccttttata aatactatat ttaataaata ataactcaat
    30361 caatataaaa tataacattg tcaatttata actagggtgc atttgtaaaa atgagatatt
    30421 agtcgctgaa atataaattg attcatcact taaatcagtc aatgtattat ttttaaactc
    30481 tgtaattgaa attattttag ttcctattaa ttttaatctt ctagcaatat taagtgtatt
    30541 ctcattctca ccacttaggg atataataat cataacatca tttttactga caatattgta
    30601 aaaagaatca tcaaatgttc ttccctcaaa atcatatatt atttcttgac atgataagaa
    30661 cattctcttt atctgttttg caaccacatt ttgaatatca cctgaaccat aagcgtatat
    30721 tgttcttgat tcataaagaa gtttacacaa tgcatcataa tctttatttt tttgatcttc
    30781 aacatactta attattgaat tacatgcaat atcaaatgac tgattttcta tgacatgaac
    30841 cttttctgat tcccagctta ataatacttt aaactcactg aaacctttta aatctaattt
    30901 tttaacaaat cttgtaatag ttgcacgaga cacattacat ttcatagcaa gttcatttat
    30961 tgacatctta ctacaatgtt ttttatttgc agttatatat ttccatatta ataaatctgt
    31021 atcattcaat ttattataat ttaaaaaaat catttcttct aatctcataa actattaccc
    31081 cctttaatta aaaatcgtca taaatattat accaatattc aacattaaat tacagcgtat
    31141 tcttatattt actatttagc ccataaaatt gactataaaa aatactgcta taattgatgt
    31201 ttaaaaaaat gtagttttaa ttttattctt ttatctaata attaatattt tcaagttcta
    31261 gtgcttttct tatacaatta tcatctttaa aatcagcagt ccttttacaa tcttttactt
    31321 taaattctgg aatagtttta aattcctcat cacatattcc attcttattt aaatacatac
    31381 aacttgccat tctaactatt agaccactat tttttaaatt aaacaataca gggtctatac
    31441 accctccatc tccgccagta gtttcaccaa ctaaagtcgc aaatttattt tctttacaaa
    31501 acattgcaaa tgactctgat gatgaatata tttttctatc aacaagtaag tatatattac
    31561 ccttaaattt tgaatcatct gtagatttta tagtatagga agtatcttca aaatcattaa
    31621 aatcttttat aatttcttta ggtgcatttt cctttacact tgctggtaaa ttttctacag
    31681 gatttaattt tatatttctt gcatttgtat atttttttac tatttcatta ttatttctat
    31741 aaattctata accatttatt ttgacatcat tttttatgag tttagatacg attccctgcc
    31801 aatacctatc acttccacct aaattacctc ttatatcaat aattaaagct ttatgttttt
    31861 ctaaagtgtt aatataatct ccaattattt ttaaatcatt atttatagag ccattctttg
    31921 atgacattga tggtaaatac atatatccaa tctgaccctt aataacatcc tttaaaatta
    31981 attctttttt tacaaaaata tcatctggca tttttatttt aagattcata gagtcatatc
    32041 tatccacaac ttttttatca ttaagaaaat cataccaatc attattagag tatgatttct
    32101 taaaaagttc atatcttttc ttattatcta ttacttctgt gtgtttatta tttaaatcag
    32161 ataatataga tgacagttct tctataaatt cttcatcatt ttcagtgttt tttattcgtt
    32221 ttgtatattc atccttattt gctaaccaat caatattatt aagcctctta tttacaccta
    32281 gatatggata tcctgtcttt acaacattat acacataatt aaagtcatcc accttttcct
    32341 cttcagacaa ctgattcttt tgagaataag tagaattacc taataatatt ataaatgcta
    32401 aaataattac taaaaataat actttctttt tcatgtaatt ctcatccact ttcagatttt
    32461 atattttaat acattgtatc attattttat tatcacctaa acagttaaaa gaaattcttg
    32521 aagatatatt ccactattat tctctttaaa aatttttatt tgtattttca aaattatttc
    32581 atgaaa
//
LOCUS       DAYAOY010000044        31549 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYAOY010000044 DAYAOY010000000
VERSION     DAYAOY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.044"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            236..910
                     /locus_tag="ACGI6B_002077"
     CDS             236..910
                     /locus_tag="ACGI6B_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751520.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            898..2058
                     /locus_tag="ACGI6B_002078"
     CDS             898..2058
                     /locus_tag="ACGI6B_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9751521.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2295..2801
                     /locus_tag="ACGI6B_002079"
     CDS             2295..2801
                     /locus_tag="ACGI6B_002079"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFG9751522.1"
                     /translation="MTFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDGLIARKLR
                     QQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYR
                     TFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELN
                     RDIKSIFM"
     gene            3087..3755
                     /locus_tag="ACGI6B_002080"
     CDS             3087..3755
                     /locus_tag="ACGI6B_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751523.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3758..6226
                     /locus_tag="ACGI6B_002081"
     CDS             3758..6226
                     /locus_tag="ACGI6B_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751524.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6466..7890)
                     /locus_tag="ACGI6B_002082"
     CDS             complement(6466..7890)
                     /locus_tag="ACGI6B_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9751525.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7909..8601)
                     /locus_tag="ACGI6B_002083"
     CDS             complement(7909..8601)
                     /locus_tag="ACGI6B_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751526.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8653..9432)
                     /locus_tag="ACGI6B_002084"
     CDS             complement(8653..9432)
                     /locus_tag="ACGI6B_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFG9751527.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9429..10199)
                     /locus_tag="ACGI6B_002085"
     CDS             complement(9429..10199)
                     /locus_tag="ACGI6B_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFG9751528.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10196..10900)
                     /locus_tag="ACGI6B_002086"
     CDS             complement(10196..10900)
                     /locus_tag="ACGI6B_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9751529.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11155..11667)
                     /locus_tag="ACGI6B_002087"
     CDS             complement(11155..11667)
                     /locus_tag="ACGI6B_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751530.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11993..12250
                     /locus_tag="ACGI6B_002088"
     CDS             11993..12250
                     /locus_tag="ACGI6B_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751531.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12393..13019
                     /locus_tag="ACGI6B_002089"
     CDS             12393..13019
                     /locus_tag="ACGI6B_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFG9751532.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13266..14045)
                     /locus_tag="ACGI6B_002090"
     CDS             complement(13266..14045)
                     /locus_tag="ACGI6B_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9751533.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14113..14574)
                     /locus_tag="ACGI6B_002091"
     CDS             complement(14113..14574)
                     /locus_tag="ACGI6B_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9751534.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14550..15227)
                     /locus_tag="ACGI6B_002092"
     CDS             complement(14550..15227)
                     /locus_tag="ACGI6B_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9751535.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15460..15846
                     /locus_tag="ACGI6B_002093"
     CDS             15460..15846
                     /locus_tag="ACGI6B_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9751536.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15849..17648
                     /locus_tag="ACGI6B_002094"
     CDS             15849..17648
                     /locus_tag="ACGI6B_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HFG9751537.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(18003..19877)
                     /locus_tag="ACGI6B_002095"
     CDS             complement(18003..19877)
                     /locus_tag="ACGI6B_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFG9751538.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19882..21546)
                     /locus_tag="ACGI6B_002096"
     CDS             complement(19882..21546)
                     /locus_tag="ACGI6B_002096"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFG9751539.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21560..22381)
                     /locus_tag="ACGI6B_002097"
     CDS             complement(21560..22381)
                     /locus_tag="ACGI6B_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9751540.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22480..23244)
                     /locus_tag="ACGI6B_002098"
     CDS             complement(22480..23244)
                     /locus_tag="ACGI6B_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9751541.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23241..23990)
                     /locus_tag="ACGI6B_002099"
     CDS             complement(23241..23990)
                     /locus_tag="ACGI6B_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9751542.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24494..25099)
                     /locus_tag="ACGI6B_002100"
     CDS             complement(24494..25099)
                     /locus_tag="ACGI6B_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9751543.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25278..25916
                     /locus_tag="ACGI6B_002101"
     CDS             25278..25916
                     /locus_tag="ACGI6B_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9751544.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            26048..26710
                     /locus_tag="ACGI6B_002102"
     CDS             26048..26710
                     /locus_tag="ACGI6B_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751545.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            26710..27891
                     /locus_tag="ACGI6B_002103"
     CDS             26710..27891
                     /locus_tag="ACGI6B_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9751546.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(28072..30732)
                     /locus_tag="ACGI6B_002104"
     CDS             complement(28072..30732)
                     /locus_tag="ACGI6B_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9751547.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30729..31412)
                     /locus_tag="ACGI6B_002105"
     CDS             complement(30729..31412)
                     /locus_tag="ACGI6B_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751548.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT        11262 a         5024 c         3940 g        11323 t
ORIGIN      
        1 aacttaaatt ataagatttc atcagctata tttttgcata ccaataatta ttaaatatca
       61 aatatatatt ctattaaaat ttctaataat ttcatccttg atttatttaa tatataaaaa
      121 tatactaact ttaatgatta agttttgaaa tataactaag aatttgataa tttattgaga
      181 tttttatact aaaatataca tactatacaa acttaattta gaagggagca ttcttatgaa
      241 caataaaata ctcgtcgtag atgatgagga aggtataata acactcttaa aagattactt
      301 tgaaatgaat ggttatgagg tttacactgc tcaaagtgga aaagacgcat taaaaaaaat
      361 atctaaaaat ccagatatca ttctacttga tataaacatg cctgaaatag atggaattga
      421 agtttgtaat agaattagag attatgtatc atgccctatt ctatttctta cagctagaat
      481 tgaaaatact gacaagataa atggttttcg tgctggtgct gatgattata ttgtaaaacc
      541 ttttgattta gacgaacttg gagctagagt atctgcccat ttaagacgtg aacaaaggaa
      601 gtcaaatcaa actaaaatta aattttataa tgatttaaca atagattacg aaaaaaggtt
      661 aatttgtata tataataatc agatttcact ttcaaaaaag gaatttgata tagtagagct
      721 tctctctatg aatcctggac aagtatttga taaagaaaaa atatatgata gagtctgggg
      781 atacaatagc aatggaaata gtgatgttat aatggaacac attagaaaaa tacgaatcaa
      841 actatcaaaa catactcttg aaaattatat agaaacagtc tggggagttg gttataaatg
      901 gataaaataa ataatatgtc attaaaaaaa gctttctttg ttcttacttt atcttcacta
      961 attatagcat cagtgcttac tactttcgca tacattttac ttaataatct atataattct
     1021 atacaagata agtatttaaa aacacctata aatacaggta ttataaccat agttcaaaac
     1081 aatgtgaacc aatttagtga ttatgataaa acattactta atataatcaa catacttcag
     1141 cttgtacttc cacttatctt ttttatagga cttttattat tagctgatat aattttttac
     1201 aaggtaaaat taaaaacacc catagaaata ctcaataaag gtgcaatgga aatatccaac
     1261 aacaatttag acttttgctt agaatataat aataatgatg aattaggtaa tctatgtaat
     1321 gcttttgaaa aaatgaggtc tgaactaaat aaaaacaaca taaagatgtg gactatgatt
     1381 aatgatagaa aacaacttaa tgctgctttt tctcatgatt taagaaatcc cctaacagtt
     1441 ctaaaaggtt actcaaacta tttaacaaaa tacataccaa caggtaagct tagtgatgag
     1501 aagattttat caaccacaca acttatgtct gagcatatta acagaattga atactatgta
     1561 gaaaatatga gtaatgctca acgattagaa gatttagttg tttcaaaatc tatgtcgaat
     1621 ataaacaagt ttatagaaaa cttagatgaa aatatatcta tcattgctaa acaagaagga
     1681 aaatctttta cacttaaaag tcaaataaat aatataaatc tattttttga tgaaaatatc
     1741 attcttagag tcgtcgaaaa tataatatcg aatgctttta gatatgctag aaataatgta
     1801 tccatactta tatatcttga acaagaactc cttacctttg taattgaaga tgatggtata
     1861 ggatttagta aggaatctct aaaattagct ttaagacctt tctatagaga taagacttta
     1921 aatgacagca atgcacactt tggtatgggt ctttatatct caaaaatatt atgcgaaaaa
     1981 catggtggct ctatttctat tgaaaataat tctactaaca gtgctaaaat tactgctaaa
     2041 ttctcaacaa gaaattaaaa tgattttgta agctttcact gttcttatat aaaatttgtt
     2101 agtttagaat ttataaaatg tatttcaatt tacaaccaat atatatacta ttttgtagaa
     2161 gttgtaagag ttgcttcaat tataatattt ggcaaaaggc atttgaaata ataatagaaa
     2221 tatatattta aatatgtaga atagtatgaa attagaattt tggaggtgat tatattaaaa
     2281 gttttgctaa taatattaca ttctctcgta ttgtgtttgc attgctaatg cttagttcta
     2341 cattctcttc actgcctttc tggatatgtt atttttggtg tggaattagt gatatacttg
     2401 atggtcttat tgcccgaaag ttgagacaac aaagtggaat aggtgcaaag ttagatagta
     2461 ttgctgattt catttttgcg gttacaattt tggttactgt cggaaaaaat attgctcttc
     2521 ccatgtatct atggtggtgt attgttgcaa tagcattgtt acggttgatt agttacagta
     2581 ttggtttttt taagtatcgc acattttcat cgcttcatac tttgatgaat aaaatcactg
     2641 gaatttctat ctttgctttt ccattactct atatttgctt tggtcttgat gttgcaggaa
     2701 ttatgatatg tatcgtggct tttctttctt cttttgaaga aatagcaatt atgataaaat
     2761 caaaagaatt aaatcgtgat ataaaaagta tatttatgtg acaacttaga atttgtgaca
     2821 aaaataattt atataataat gaggaagtaa aacataaaaa attatccaca agtttatgtg
     2881 taaaatagtt ttaaagcaat agagttgtat tgtaaagcct ttagtgcata aattacattt
     2941 taaattaaaa atgatactga aactgcttat cagcactgcg aattatccgc aaatagtgaa
     3001 gactttttag atttaagcta aaagttggga aaaaattgag ttttaaaact tacactcttc
     3061 ttatatagaa aaggagagtg ttttttatga ttataaaagc aaaacaatta tcaaaaatat
     3121 atggttctaa taacaataaa gttatagcac ttaataatgt aaatttagaa ataaactcag
     3181 gtgaatttgt ttctgtaatt ggaccttctg gaagtggaaa aagcacactt ttgcatattt
     3241 taagtggatt agataatcct acctctggac aagtgttact tgatgataaa gatatatata
     3301 aacacactga aaaagaacta tctgcactta gaagaaaatc ctttggattc gtctttcaac
     3361 aatttaatct tttacctgtt cttacagcat ctgaaaatat ttcaatgcct gtacttctag
     3421 ataaaaaaca acctgataaa gactatttaa atgaaatttc atcattactt ggaattgcag
     3481 atagactcaa tcatcttccc catgaacttt caggcggtca gcaacaaaga gttgcaattg
     3541 ctagagcact tattgcaaaa ccagatataa tatttgctga tgagcctaca ggaaacttgg
     3601 acagtaaaag tggcagtgaa gttatgaatc ttcttataaa aacttcaaaa cagtttggaa
     3661 agactttagt ggtaattact catgatgata gaattgcaaa acttgctgat agaaaaattt
     3721 caattattga tggtgttctt atggaggtga aatagaaatg aagaattacc tttcactttc
     3781 aataaaagaa ttaaaaagcc agaaatttat gactacattc atcattatag caatagtact
     3841 atcaactatt atgacaactg tggttggaca gtcaattggt atcctacaaa atttaagaat
     3901 tgaacaagcc cgttctttta atggtgatag acatgtatcc tttcaccaac tcactaaaaa
     3961 tcaagttgat gacataaaga aagatgatag agtttatcaa gctggtactt caataactat
     4021 tggcagtagt aaaataaagg attctggtat ctctgtactt gtaaaagagt atgataaaac
     4081 aggactttct aactacccaa aattaatgaa attaaaatct ggacatttgc ctaaagataa
     4141 aaacgaaata gccttagatg aaaacacact aaagcttatg ggtataaaac caagactagg
     4201 agtgactata cctatgaatt tagatatttc acttttaaat gataccatac caccatataa
     4261 ttacacagct aactttaaat tgagtggcat tttagaggat gattatacag gatatgtaag
     4321 tggtatagta aatggtattg taggtaaagg tacatcagaa aatctattac ctaagagata
     4381 tattttatcc tctttggatt ttaaaataaa acagcaagaa aagtttcaag aaatagtaaa
     4441 tcaattagct aaaaaaataa atctttctca caattcaatt caatataatt ggatttactt
     4501 aaatgctctt aagatacagt ttgaaaaaga tgaaaatagt tctaatagtg atggtatatc
     4561 tatgattata ttggtatctt tatttgttgc cttattggtt ttactggctt ctggacttgt
     4621 aatttataat attttaaaaa tctctgttac aaagaaaatt aaagagtatg gatgtttaag
     4681 agcaatagga gctgaaccaa accagatata taagatagtt attttacaaa tcttaatttt
     4741 atgtactgtt gccataccta ttggagctgt aattggaatt atatcttcta aaggcataac
     4801 tggaatggtc actaatatct taaatccaga tatacttcta gcaaatgata ataaagaaat
     4861 tactgagctt atacataaaa atacaacagc atatatattc ccattagtat taagcactaa
     4921 tgtatctttg attttttcat ttatttcagc attaccatca gcaatatatg cttcacatgt
     4981 ctctcccaaa attgctatgg ttggtagtac taccaaaatc aaaagaaaaa ttaaaagaga
     5041 aaaaacaata aaaaactttg aaagacatct agcatggcta aacttaaaaa gaaataaagg
     5101 aagaactata ataactatac tatcattatt tatgagtata acagtattcg ttgcactgag
     5161 tgagttctct aatgtattag atgtatctag gtcagtaagt aatttaaaag aaggagattt
     5221 ttccttaaca aatgaaatat ctggttttga taaatcaaca ctagataaaa tagctaaaat
     5281 gaaaaacgtc aatcgcactt cctttataaa gtactctgaa tacaaacagg gtgaaataga
     5341 tacagatata aattttgaaa atagtggtga gatgttaaaa atcattggaa ttgatgagca
     5401 aactttaaaa gacttaatgc cttctattac agattctata ctagaagact ttaaaaatgg
     5461 ttctatctgt tttataaaaa atccgattgc tatttctaca ccaggtgtaa aaactagata
     5521 tacaaattta aaacctaaag atagtatcac aatcaacaaa aaacaactag atatatatag
     5581 tacagtagat aaaatgttct ttttacaagg caatggctgg ataaatggtg ttgatgttat
     5641 tgtatatgat tctgtttata acactttgac aaataaaaac aagttcaacc agataaatat
     5701 ttatgctaaa gataaatcaa aattagaaca aataagattg tctattgaac agatttgtga
     5761 aaataatcct ggctctcatt ggatatcata tattgaatca gataaacaac ttaaagagag
     5821 ttttaaacaa atagaatttc tagcatgggc agttatatta tttgtgggat tgattggtac
     5881 tttaaacatc attaacacaa ctcatacaaa tataaatact aggactaatg agattggagt
     5941 taaaagagct attggtatga gtaataggag cttatataaa atgtttttat gggaaggtgt
     6001 ttattatgga atttttgctg ctatatttgg ttcaattgct gggtatgcca gtgctattat
     6061 cataaacatg gcaactatag aaaagcttga tttcactaat ataccaatta cttcaatttt
     6121 acaagctact attatttctg tacttgcatg tattatagct actcttattc cgttaagaaa
     6181 agttaaaaag atgaatataa ttgactgtat agatatcaat ttataactat tttacagata
     6241 ataatataaa ttaaattatc aatgactgtc tctattttct ttcaaatcta actaataaat
     6301 ctttttaact ataatataag ctttaaaaaa ggcctgtctc aaaatagaga taagcctttt
     6361 ttcattgtcg aaataatgaa aatatattta acttcacaaa aaaagagtgc ctcacgaatt
     6421 aaaaatttcg ctttgagaca ctctccttta atttaaaata tatagttaaa tgctatgcca
     6481 aaatttcaca catgctccac ctgattttga attttctatt ttaacagaac ctccaagctg
     6541 ttccactaat tgtttgacaa tgtaaagccc aagaccagaa tgtccacctt ttgtctgcct
     6601 agcttcatct cctcgataaa atttatcaag agcctttttt aaatctttag agctaaaccc
     6661 tctaccagaa tcacaaatct cataagaaat acaatttttt tcatccttta cagtaatact
     6721 aatattacca cctgatggag tgtattgcag actgttggat atgatattat caaaaattcg
     6781 tgtcaaccta tcaacgtcga tttgaattgg ggattgtata ttcccctgca ttttcaatat
     6841 aagttcaact tccttttgat gagcctgtaa ttcatagtct tgcacctttt gcctcaaaaa
     6901 ttcaggtaaa tttataggaa caagatttaa ttgtatattt gacttttcta agtcagacgt
     6961 atattgcatc ttttggacaa gagcagcact tttttctgta ttttctctta tcactgtaag
     7021 atacctatga agtttttcat tgtcatctgt attattacca ataagtgcat ctgtatatcc
     7081 caaaataata gaaagaggag attttaaatc atgtgctaga gcttccacca tttcaacacg
     7141 ttcctgctcc atcttccact gtgcagataa agagcctttt aattcgtctt tcatttcaga
     7201 aaaggcacta caaagtttac cgagttcatt atctgaataa tagtcgattt caaaatcaag
     7261 gtccttttct tttatcttct tggacgcatc aatcaataga tgtaatggtt gattgatatt
     7321 ctttacaaag cgcttagaaa gccatctagt aaaacccacg atatatagaa atggagaaaa
     7381 caaagccatt ataataactg caaatacaaa tctccctcta atatttgcaa aggtgatttt
     7441 cacttggtag aatagaataa cagcaccctc aatctttcca ttatcccctt tgattggaac
     7501 agtatggata taaccacctt tacgaacagt tttattgata aaattattaa ataattcttc
     7561 ttttgtttta aatagttttt tggggttagt accatataaa atattgccat tattatccac
     7621 aacttgatat agcatatcat ccccacgaat cgttcttttt aaaccttctt cattcgattg
     7681 tgataacaaa gctatgtttt tttcattgat gtatttttca attctaggaa cttgtcgctc
     7741 ataatagttt ggtggataga tatccttatt taaggaatga atatacaaat agagagcaaa
     7801 tacataagta atcactgtgg caactatact ggctattata ataaaaataa aagtatgtct
     7861 aaattgggta ataagggatt gatttttcat ttaataacct ctctctcttt acattttatt
     7921 ccacttatac ccaatacccc aaactgtgga aatatattct ttttctggtg tcacagcaga
     7981 aaacttagct cgtatttttt ttactcgttc aacaacagtt gaggaatcac cttcagaatc
     8041 acacccccat actttttcat agatttgttc tcttgagaaa acttgtcctg catggagtgc
     8101 tagtaattca acaatatcat actctatttt tgttaaatca atattcttac catcaatttt
     8161 aattgcacgt tccttcatat ataagcataa cttcccaaaa tacaaagtag atctcttgtt
     8221 ctcttcattg atatactgtg aacgcttttc cctacgcaga ttagcttcaa tccttgccat
     8281 aagttcacgc agtccaaatg gctttgttac ataatcatcc cctccaaagg tcaacccttt
     8341 gattttgtcg gtttctgatt gctttgcact taaaaatata attgggcata aaacatcatc
     8401 tctaattgcc tgacatacct caaatccatt cattttaggc atcataatat ccagtataat
     8461 aaggtcaggc tcctgttttg ataactctat acctgcctga ccatcataag ctatcaacac
     8521 ttcatgtcct tttgccttca attcatcctc aagtagcatt actaggtcca tttcatcatc
     8581 aataatcaat attttactca atataacacc acctttctct tatgggaagg tcagtattaa
     8641 aacaatattc gtctactcat aacttttcct tccctcccac attttaaacc aaataacgcc
     8701 tcctatcaag aatataatca aacctattgc tactactata agctctatac tagctgtttg
     8761 catcacttta gagtaaaatg cctcagttga aatagttcca gcttctttaa taaaataaat
     8821 tcctggaagc atacctagtt ttactggcca tgtccatgga acaaatgccc atactttttc
     8881 accaacgcta gttaatccaa gtattgctgc catcaaaatt ccaccaatac taataccaat
     8941 agatgcaccc ataccccatg caaaactcac ccaaagatga atagctaata gtggaagtgt
     9001 cccaatcacc acaagtcctg ctgctgacag gaataaccat attgttgctc cagatggcac
     9061 tgcaatactc attcctatgc aaagtatcaa cgtagcaata aatgtgcata tagttaggca
     9121 aaacagaaga aaaagaaatt ttcccagata taatctgaca cgagaaatac ctacacataa
     9181 aaaaccatta aaatttccag caagctcttc ctcctgcact ataaatcctg ctaaaatacc
     9241 tactccaata ggaatgatga atacagtcca tactgtaaaa aatccttcaa aagcaaattc
     9301 ttgtgtactt ccagatttag ttgtgagata aaaaaccaca cataatgaaa aaataatagg
     9361 catacaaaga gtaagccact ggacagctgt cctctttgct cgaagccatt ctgatgataa
     9421 tagttttatc atttgtcatc cctcctattg aaccaagctg cagtaagtgc tgttaatact
     9481 ataaacacaa ccaaagaaac aacaatccca ataggaatca ctgaagtatt caaaagtggg
     9541 tctccagctt ctaatatagt accgttagga tgtatgttga tgattgggca taccattcta
     9601 actgcccaac tccaaggtac tgcaatccaa aatggttttg tcgcagcaag aacaccaaat
     9661 atcataccta aagctccaac tcccatactt aggaacatac ctttccatgt agctagccat
     9721 agctgaagtg gaagtatagc aagagaaaca acccagcata caatacttgc ccctataatc
     9781 tgtccaaaag gaactggacc agctttcaat attaaaccag ttataattgt tacgactatt
     9841 aaaactagcg ttgaaataaa actgtagact gccatagcta taactttatt aatccaaagt
     9901 gtcataggag acacattgtg tgtacgtaaa gcacgataat ttccagcttt tttttcctgg
     9961 aaagctacca aagtagcgaa caatgcaaat ccaataggca gaaataaaaa tgaccaccag
    10021 ttaaaaatta atgctaaaag attttgccac gcccaagaag ttgtttgaga taatggattt
    10081 tgcatgagtg ttgattgcat tataaatgca tatgctgcaa aagacactgg gaaaaaaaca
    10141 ataagtttac ccataaatgt tcttttatac tttaaaagtt ctgattgtaa agcattcatc
    10201 tgttttgtcc tcctttaaga ataacatctg taaaaaattc ttctagattt tcattagggt
    10261 caggcattcc ttgaaataat agtcgtcctc catttataat tcctatatta tctacaactt
    10321 gagcaacctc tgatagaata tggctagaaa gaattacagt tatacctttt ttgggaaaag
    10381 aagcaatcaa ttcacgcaat tcttgaatgc caaaggggtc aagaccattg gttggttcat
    10441 ctagtattaa caactctggg tcactcaaaa gtgcaatagc tattcctaac ctttgtttca
    10501 tacccataga aaattgtgaa gctctctttt tacctgtatt tttcaaattt actatttcca
    10561 acacttcata aattttatct ttaggtattc ccaatatttt tgtatgtact aacaaatttt
    10621 cctctgctgt aaggtttcca tacaatgcag gggattctat taaagaacct atttttgcaa
    10681 gagattctcg tttccaaggc tttctatcaa aaataatacg accatttgtt gggcgaagaa
    10741 gaccaacaag cattttaagt gttgtggact ttcctgctcc atttggtcct aaaagtccat
    10801 atatactatt tcgttcaatt tgtagagaaa cattgttaac tgccatttgt tctccatatc
    10861 tttttgataa gttttttgtt tcaagaatta aatctttcat attaatttct cccttcttgt
    10921 aataccttaa atctgttact aagagaatac taacatgaaa aaataacaat tctataacac
    10981 ctactaattt taataaaatt taaaataaaa aatagagtta ccaattttat tttggcaact
    11041 ctatttaagt ttatgtttta cagttatatt agatttaaac ttagtttcat tatcacacta
    11101 acttcaaatt tactctataa tctttaaatc aaacactaat ttattaatca aacactaatc
    11161 tattaattta tatttcttta gacatctaat atagtttgct actaaataat aattatttat
    11221 atcatattta tctaatcctt taaaagaagt tatttcatca ttaagctcat cactaccttc
    11281 tacaaaaaca caacctgttt caaattccat acctacaata atcttacagt cactataaat
    11341 ttcctcttga gtatcgtaca atttttttgc atttccttca tttggaattg taaatacaat
    11401 aggttttaat tctttatcac tggaaatctt ttgtgaaaaa ggcttgttta acttaatatt
    11461 attgataact acatgtttaa attcctctga agtttcttct actattacat attttcgctt
    11521 taaaaactct attatttcta aaacagactt agaatttact tttaaaatac ttttatattt
    11581 ttcatataaa gatttccact gttcaatttt ttcctgtggt atttcttgga tatgtgtact
    11641 tattttttct tgagatattt ttttcaatta aactctcctt tcaatactta aatatcctta
    11701 tgaatctcac atgactaaag ttacaaggtt tctacttaat agaattttgc atacttaaat
    11761 atgtcttatt aattctctac aggatatctc cgtagttcct acgattctta cataagttac
    11821 ttattttaaa ttgttattag ttatattcta aaaattattt attttatcac taatataata
    11881 ctcttaaaaa ctttacaaat caatttaaat ttgctacctt ttatttataa caacttatag
    11941 tacatttctc ctatgctttt caatatatta cttttaaaag gaggttttca ttatgatttt
    12001 aaatagagct gagcttttcc gaggatattc tttaaataga tttaacgaga tatgcaatat
    12061 attatcagta aataaaatta aatataagta taaagtgaaa aataatgctt actctaaaaa
    12121 accttttttt aatgaaataa cacttggcaa tttaggccaa aaggaagatt tctcttatga
    12181 gtacttaatt tatgttcata aagacgatta tgaacaggca gagtctctaa taaattctaa
    12241 gttgcgatag attatctata aaacatgaca ttctgttgtc atgttttata tgttattata
    12301 ctttcaaata tgctactata ttttcaaaat tgtgtgcttt aaaactagct ttttttagta
    12361 atttaaagct taaaactaag gagactcgtt atatgaaaat aaatataaaa tcaaagttaa
    12421 gtgaatttat taaacagaac aatctttttg atgacagcag aataatatcc tatcttccta
    12481 atataacaat agaaacagac aaaataaaaa ctataatgat taatgaggtt gtcccaacta
    12541 acaccaatga cgatttttat agtgtagata aagatgctga ttatttaaaa actactattc
    12601 ctttatttga cagtgcagga ataaaagtat ctaacataaa tgatatccta gatatgggta
    12661 tctatataac taatgcagta aaacttccta aatcagaata tacaataact cgtgatacaa
    12721 ttaaattaca tatgcctatt ttagaagaag aaattaaatt atttaaaaac ctagaggtag
    12781 taatgttaat gggagatgtt gcaaaaaaat cttttaatat gattactaaa aagcatataa
    12841 aaaagaatgt catcccttcc atttcaacat ataaacttcg taataatgaa ctttattatg
    12901 ataatttaag agtatttcca tcatatatta tgactggtgg aaacatactg attgaaaaat
    12961 caaaatttga aatggcatca gaagatataa aaaaaatgtt cgagattata aacggttgat
    13021 attacaaaat aataaaaact aatataaaat aaaaagtgcc tcaaaaactt ctatttatga
    13081 ggcactttta ttttatatta gtgtaactat atactattaa cataatttat tatttatatt
    13141 gttcttttta atctctattc catttagtct aggtaacatt actctttaca agattacttt
    13201 atatttcttt tatatctcta acgtttttat ttaataaaac aattattaat acaagtcaaa
    13261 tatatctatt tatctaaaaa tatatctttt acagtaatat ctggatggtg atacttcttc
    13321 ctattcgtta aaaaagattt ttttacatta atagcctctt ttggacacca gtgaaaacaa
    13381 gctaggcatc tttcacactc attaccccac tttggctttt tatcaactat acttatattc
    13441 tctacaggac aaaccctttt acatgtccca catccattac atttattttc tgcaaaaaat
    13501 ttagtagttg caatttgcat attcttacct actaaacgaa gtacaacact atctatatgt
    13561 gactttaaat tgttttttcc ttctatattt ccaatctcca agttattgat acattttgca
    13621 atctcagcta ccctattagt ctgatttttc aatctttctt cttcaacatc tggaggtgtt
    13681 atcaaagcat ttccaggcat attgattaaa aaaccagcag ataatttttt cccccttttt
    13741 ttaagtaact tatctatagt aaataaacta tgtccagcta ccccattaca agtggcaatg
    13801 gcatatatat gagtattaat attaaaattt aatttctcta caaaagactt tactatatca
    13861 ggaacatcag caaaataaac aggaaaaaca attaccaata tatcttcatt tatgtctata
    13921 ttattttctt ttctatgtat agctaagggt ataacttctt tacttccatt taacgtttct
    13981 tttaaaatat ttgaaactac tagcgaattt cctgttccag aaaaataata aatcttacta
    14041 ttcattctta tccctctttc tatatttcta catcatcaat agttaaaaag tctcttaaaa
    14101 taggatttat atttactttc caattctatc caaaatatca gtaaacaaag gcaattcttc
    14161 atcagagata gatttaaatg tctctgttgc atatatactt gcaatttcat ttaatggttc
    14221 ttctaattct ctacctaaat ctgttaggaa tatttgaaac ttacgtctat ctttaacatc
    14281 tactttacgg acaattaatc ccttattttt cataacatct aaaattcttg ttgtattaga
    14341 tttatcctta catgattttt cacataaatc tatctgagaa atcccatctt gctcccatag
    14401 aaaccttaat aacgcataat ggtcagttgt tatatcatat ccattttgtt taaacatctt
    14461 ttttaaatca tcttttactt ttaacgaagt acttactaac ttagttgtag cttcatatag
    14521 tttctcaaaa tctttcattt tacctccatt taaataatcc cttgctcctc ccatctatca
    14581 attattccct tatattgctc aatatcttta aaagccattg gctttaaaac agcatttata
    14641 gcaataaaat tatagatttg agaaagcaca ggtggatttt tcttttcctc tatgcaataa
    14701 ataaattttt tagatacctt ttgtatcttt gattctactt tttcagttat aggattttga
    14761 ttaaatccag tttttataga taaactacca caaacataac ctcccgaatc taagaccatg
    14821 gattttataa aagaaattgc ttttgaacca cgtgcatttt catatgtagt aactgctata
    14881 catggcttac gatacaataa tctttcaagc atcatatgtg ctctatctgt aaacaccttg
    14941 aaaagtccag ttacactact tccataagtt gggctaccaa atataacacc atcactctta
    15001 gtaattatat catgaatata ctctacttta tcattttgat tgatacactt gcccatttta
    15061 taacaattta aacaaccaat acaatatgat ggatttacct cacttaaatc aaaataatta
    15121 atttttacat ctggtattaa tctttctata tctttataca agtaagttaa aacttttgat
    15181 gtagctccat tttttctagg gcttccattt ataactgtaa ttatcatttc tatatctcct
    15241 ttttagttgt tatatcaaca ataagtatat caactataat tttagctgtc aatataaacc
    15301 atttatttaa agctatactt acacagctta aataaaagtt ttatttaact acatgtgctt
    15361 atttaatcaa ctataaatga attattttta ataattgaca aactaaatta atgataatat
    15421 actttatgta ttacaaatgt aagtagaagg aggtatagta tgaaaaagtt accacatatt
    15481 tcagaagcag aatatcaagt tatgaaaatt atttggaaat atgctcctat cagcactact
    15541 gaagtaattg aaaagttggt agaaacaagt acatggagtc ctaaaacaat tcaaacaatg
    15601 ttattgagat tagttaaaaa aggagcttta acatatgaaa aaaatagcag agtattcgtt
    15661 tatactccac ttgttaaaga agaagaatac gtagctacag aaagtagttc ttttttaaat
    15721 cgtttttata atggtactct taattctatg gtactaaact ttttagaaaa tgataaacta
    15781 tctgaagatg atattgagga actgagagaa atcttaaata aaaggacgac taagggggat
    15841 aaataaatat gtatgcccca ttctttatac gatttttact cagtacatta accttatcaa
    15901 tattaattgc tgtaatttta cttgccaaaa aagtatttaa aaaacatatt tctgcaaagt
    15961 accaatacaa tattggattt atattgctta taatgttaat aattccattt gtccctctta
    16021 aatatattaa tcttggaaat gtatttaact atttatatac ttttaatcaa cttggaagca
    16081 gtactaatag gacttctatg ataaaaaata gtccaaattc tataattaat aactcaaatg
    16141 cattaaacga tttttcagta tccctaaacc aagttaattt tgatagttta aatattttat
    16201 tactaatatc atggataatt ggaattttaa tcatgatagc tattactatt cattgcaaca
    16261 ttaaaataaa aaatatgaaa aaatcaatac aaattttaag agatgaggag atagtattta
    16321 tatttgaaaa gtgtaaaaaa gatattggta taaataagga ttttatacta tgcaaatcac
    16381 ctattataaa tacacctatt acatttggat actttaaacc acacattata ttaccagata
    16441 agtctatatc taaattatct ttaaaagata taaaatatat cttacttcat gaattacaac
    16501 actttaaaaa taaagatata ctgataaatt atataatgtg cttccttcaa atattatatt
    16561 ggtttaatcc tcttgtatgg tatgcattta aagaaatgcg tatagaccgt gaaattgcat
    16621 gtgatatttc agttttaaaa aagcttgata aagatagtca tatagaatat ggacaaactc
    16681 taataaactt tgtggataat atttcaagac catcaaatct tacacttaca tcagatattg
    16741 gtgggtcaaa aaaacaaata aaaaagcgac tattgaagat aatggatttt cataatgaat
    16801 caaatacgtt aaaattcaaa agcattttaa tttttacatt gatagcttta attgtatttg
    16861 gaaatattcc caaactatct attatggcag atatcaatga aaaatataga cctacaaatg
    16921 aacaaactgt gtacgaggac ttaagtcagt attttaaagg ttttgatgga agttttgtta
    16981 tgtatgattt aaaatccaag caatatcata tttacaataa agaaaaaagt aaaacaagaa
    17041 tttctccaaa ttctacttat aagatttata gtgccttatt gggattggaa aatgggataa
    17101 taacaagaaa taattcacat atcccttggg atggaaagca tcattctaat agttcttgga
    17161 atgaagacca agacttattt tctgctatgg aaaactccgt aaactggtac tttcagactc
    17221 tagataaaaa aacagggcta aaaaacatac aaacttatct taggcaaatt ggatatggca
    17281 attatgatgt ttcaggtggt atctcaaact tttggctaga atcttcatta aaaatatcac
    17341 ctattgaaca ggtagaatta cttgaaagtt tttatacaaa taagtttaaa tttgaagata
    17401 aaaatataga aacagtaaaa gattcattgt taatatcaaa aaaggatggg gtatcacttt
    17461 ttggtaaaac aggcactgcc aatataaatg gaaaagatgt aaatggttgg tttataggtt
    17521 atgtggaaaa aaatgaaaat acttatttct ttgctacaaa tattcaaagc aaagattatt
    17581 ctaatggtag ttcagcatct aagataacat tatccatatt aaatgacaaa aaaatctata
    17641 atccttaata tatttactgt acattctaaa aaattcacat aattttcata ttaattatca
    17701 attaaattca tatacaaggc ttattatata aacatagaag atagtaaaac tagcttaaaa
    17761 acataatata ataccctata caaatagtgg caactctccc tcacatcctc aattgttgcc
    17821 actatttaat tttttgtatg aagttaaata tattttcatc atttcaacaa tttgggtgca
    17881 atacaatctc tattttgaaa caacctcttt aattgtttta gagtttatga attatttctc
    17941 atactttaaa aagattgaat tactcttgat aatcttcttt tgtagtcatt acatatcacc
    18001 tactactcaa cacatattct ttgacctatt tttacatcct tgtttatatc caaaatattt
    18061 accttcttac catctggaaa caataccatt gttaaagtag actttcctaa ttcatgtatt
    18121 ctctttaaat caacattaac aagaacatct ccctgtttaa ccatatctcc ttgtttaaca
    18181 gcaatatcaa agccatctcc atttaatgca actgtatcaa ttccaacatg aattaatact
    18241 tctccattag ttcctttaat cccaaatgca tgtccacttg aaaccactgt ttctaattta
    18301 cctgaaatag gagaaacaat ttttccatct ataatatcaa tagcaaaacc atcacctaac
    18361 atttttttag aaaatgtttc atctggtaca ttttctaatg caacaatttt ccccttagct
    18421 ggagacacaa agttttcatt attttcatta aacactaatt cttcgtcttc atcttcattt
    18481 atagcttctt taggcactcc aaataaacat gttacaataa agcagaatgc aaccacaaat
    18541 attgatgcta ataagtatgt gattaaatta tatccatcat agatatacat taaataagaa
    18601 ggtaaaactg ctaatccata tgaattagat tgaatatgta aaattgataa tattgctgct
    18661 cctaaacctg aagatgcaca cataataatc aaaggcttta aattatatcg aagctgaatc
    18721 ccaaataaaa caggctcact tataccaaaa agttgagata gcattgctcc aactgcagtt
    18781 tgtttaactt gattattttt agattttttc ataaatgcta gacatgttcc aatattcgca
    18841 aatccataca ttgcacaaag agtaataagg gcattaaatc ctgtatttgc taatagtgat
    18901 gtttcaatca ttacataagt atgatgtaag ccagtaataa cagctaatgg atatgtaaat
    18961 ccaataataa atcctccaat tcctaaaggc aaatcaatca ataagctgat gatatcaacc
    19021 atttttaatt ctattgtatg cattataggg cctacaccta acaaaataac taacattgtt
    19081 attagcataa caaaaaatgg tgtgaaaata aggtctaata cattaggcat atgtttacgg
    19141 aaccattttt caagattagc tccaataaat gctgtaacaa tagctgttaa aacacttcct
    19201 tgacaaccta caataggaat aaatccaaat gccataattg cttcaatttc atttgatggg
    19261 tcagcaacag aatatgcatt aggaagtatt ggggaaacta acataagtcc aataacaagt
    19321 ccaataacag gtgttcctcc aaatacttta aatgctgacc atacaataat tgctggtaaa
    19381 aaagcaaatg ctgtttctgt aagaaccgaa acaagtgtct gaatatacaa tggaatattt
    19441 gcagaagaca ttccaaataa tccaagtaca ttatcattaa acaaacaacc ctttaatccc
    19501 aagaataatc ctgttgctgc aataactgga ataataggaa caaatatatc tgctagagtt
    19561 ctcataactt tcttaaatcc agtttcatta tttttaacta attcatcttg ttcttttttt
    19621 gataaagttt ttaatcccat tttttcaact tctgcataaa ctttattaac tattcctgtt
    19681 cctaaaataa tttgatactg tccagaagta aagaaaaccc ctttaacctc atcaactttt
    19741 tcaactttct tatcattaat aatttctcta tctttaacca tcactcttaa tcttgtggca
    19801 caatgtgtaa tagaaataat attatcttca ccaataattt taataagctc atttgctatt
    19861 ttattatatt tattcatttt tctacctcac tttcaataat actgcatgaa atggtttcaa
    19921 acgcactgtt ttatttaata gatttatatc atctaaattt tggaatacaa tatcatattt
    19981 ttcttctact cttatttcta caggtttatc agaaaaatta taataacaat caataactac
    20041 caaatctaat tgccttctat acgcaataat atcatcactt tctatatcaa taggttctat
    20101 tgaaccataa atcaaacaat cagaatactc actatcttgt ctcaacttta tcatcttttt
    20161 ataaaattct aaaatactct ctgaattgtt aatctgactc tgtgcattaa tatctttata
    20221 attttcaata acctttaacc atggtgttgt atctgaaaaa cctgcatttt ctttatctgc
    20281 ccatggaaat ggcgttcttg cattatctcg gcttcttaaa ttcacaaatt ctaacgcttc
    20341 ttcttctgag taaccttcat ctattgaacg atagtattgg tcaatgctag ataaatcatt
    20401 aaaatcattt atggtatctc ttttaaaatt tgtcattcct aattcctgtc cctgataaat
    20461 aaatggggtt cctcttaaaa agaaatacat tgctcccaac atttttactg catcatcatt
    20521 cctttcgtat tgcaaaagat actttgttgt agctcttggt tggtcatgat tttcaataaa
    20581 attagctgcc catccatgtt cctgaacata catttgacta tctaaaatgc attttcttaa
    20641 atctggaatt gtccatgaaa ttcttttaaa ccactcacta ccagaagcaa catctaagtc
    20701 agcatgttta aagtcaaaga ccattgaaaa gaaaccatct tctccaataa aatcacctaa
    20761 ctggtcatag ctaatacctg ctgcttcagc aacagtcata caatcatatt ttttaaacgt
    20821 attttctttt aattcataaa gcattttttc tattccaggc tgatttctaa ctgtttttgt
    20881 acatttaaca agaccatcaa caccatcact ttctaaactt tgaaaactta aattcttttt
    20941 gatgaatgta atagcatcaa ctctaaatcc tgccactccc atttgagacc aggtattaat
    21001 catttggtaa agttcacttc ttaatattgg attttcccaa ttaaggtcag gttgtttttt
    21061 tccaaaagaa tgaaaataat attcgtctct attctctacc ttttcccaaa cacttccacc
    21121 aaaaacagac ctccagttat ttggcaaatc gtttcctttt ttaaaaatat aataatcatg
    21181 ataaggactt tctggatttt ttaatgcttc ttgaaaccaa ggatgttcat cacttgtatg
    21241 attaagaact aaatctaata taattttgat tcctctatct ttactttttt taattaactc
    21301 ttttaaatct tccatacttc caaattcact tgcaatatcc aaataatcac taatatcata
    21361 cccattatca tccataggtg atttaaaaat tggacataac cacaatgatg taatacctaa
    21421 atcttttaaa taatctaaac gagaaataat tccctgtaaa tctccaattc catcattatt
    21481 actatcttga aagcttcttg gataaatttg ataaacgatt tctttttgcc accatttctt
    21541 ttccataaat tactcctttt tatatgatat ttcttctttt taattcttta taaattccat
    21601 tatcgaatat gtcctcacat atagatgtcg ctatatcttt aagttgttga tgactatttt
    21661 tcattgcaat tgtaacgtct gatgcttgaa acatgacaat gtcattttga ccatcaccaa
    21721 aacatatggt ttctttttga gttactccta atctttcttg taatctctta attgctttgc
    21781 ccttatgaaa atctttttga ataatttcat aatactgact agaaatatct ctctgtgcta
    21841 attccatttt atcttgtagt atatctttca cttcgtcaaa taccttttca tttgaccata
    21901 aacaaatttt atgtatatct tgtgacttat actcttcaat gttattttca tatgttattt
    21961 tttcttggat atgttgttta tttatacaag aattagttcc tttcaactga ttcattgttt
    22021 caaatatttc tttggctttt tgattcatga aaactttttc ctgactctca atcgaaaaag
    22081 caacttctct attttttaat aaacaaacaa cttcctttat caatctttga ttaaaagatt
    22141 gattataaag taattcacca tgatattgaa tgtaattacc tcctcctgca atatatccat
    22201 caactcctaa tgataaaaca tcatcttgta ttgtacccat acttcttcca gtacaaataa
    22261 caacactgca atgattcttt tgacataaac gtatagcatg ctttgcagat tctgggattc
    22321 catatacctc atctctaagc gtaccatcta tatcaagtat taataatttg tacaatttca
    22381 tcttcctttc ttactatcaa aatcataata gcattcaaaa aaaaagatat caatattttg
    22441 atatctttta aaaaatatgt tcatttttag cacaaatttt cattttcggg gaatatgttc
    22501 actttctaaa tacatcttgt atttaatata aagcagttca attaaaataa aataaggtgt
    22561 tgttacatcg tagtccagtt gtttatctac agaaatagat atagattgaa tatacaggtt
    22621 ttcatcacat aatcttgcta aaggattact tatcattttt gtaatagaaa ccatataaat
    22681 tcctttcatt ctcatttctt tagcaaaatc aataattgta gatgcatctc cattcaatga
    22741 aataaagaaa atcatatcat ctgtttttat aaaatgatta agtgcctgaa tcatatcata
    22801 accataaaca tcatatataa ttttccctgt tggtaagaaa atccttttca tctctttagc
    22861 aactctttcc tgagaataac cttctccaaa gacgattata cgatttgcct ttttcattct
    22921 ttcaaacata atcgaacaat ccttcttctc tatatcattt atcaccttat tatagcaatc
    22981 cattaattgg tgtttacttt gatttttata ttctacttct gtttcattta agcgtataat
    23041 aactcttaat tcactatatc caggtaattg tagtttttga gcaaaacgag aaagtgcact
    23101 tgtagatata tggtatttgt ctgcaaattc atctatagaa agtttaatac aatcttcttt
    23161 atgattaaga atacactcac agatatactt atcatttgct gtaaatgaat tataatattt
    23221 attgaaaagt ccttcaattt tcattctttt ctccttctat actctatgta attcatatga
    23281 agcaaatcta atagagcata aaatgctcct acgacttcat aagatatagc ttgtaatgta
    23341 tcaagttttt gagtttcaac atacagatta tactcacaaa gtgttgcaat tgtattatta
    23401 tcaagcctag ttaaagaaat cgtatgtata tgattgttga taaattttgc aatttcaatt
    23461 cctgcttttg tttctccaga caaagaaatg ataataaata aatcactttt ttgaaatgat
    23521 tctctcataa attcaatttc acctatatca aataagtcaa taacacattt acctactgaa
    23581 agaaatattc tctttatttc ttgagctaaa cttttctgtt cattacctgt cccataaata
    23641 taaattgttt ctgcatcata tattatttta caaatatctt gaaatgatat tttttgtaat
    23701 tcattcatta atagtcgata attctcgcaa acatcatgaa aattattatc attattaaca
    23761 aattctagtt gattattttt aagtaaatac tttaaatcag agaatgaatc taatccaatt
    23821 tttctacaaa gccttaataa agtagctcta gaaacattac attcatttgc aattttttca
    23881 cacgaaaaat tacggtattc tctttgacaa atcaatattt gtttaataat aattttatca
    23941 ttttcattta aattatcata attttggtta actaacgatt ctaatctcat tttttccact
    24001 ccctaaatat tattttaata tacattagtt gctttttcta gggaagagtt ttaaataata
    24061 aggataaaat aatcatttga ttttattttt attaaaataa aaataacctg ctaaaagcag
    24121 gcaccaaaaa gtacttatcc tttcgaacat gtactttaga tattgctttc ttcggaagct
    24181 tcatctcttc atactctaaa atagagtttt ttctaactca aaagtatttt ccatggtaga
    24241 aattacttta attcatctgc catttatttt ttatcttatt taataattac tactacttga
    24301 atatttactt ctttaagtaa atttcatagt taacaaagct aaaattatta ttatagatgt
    24361 taaaatacaa aattgtaagt attgttataa atatgcttac agctacaaat atacatgata
    24421 tattaaaaag aagctgtaaa ttattatcat acatacaagc aactacgcct ctattaatcc
    24481 atgttaattt ttattattta atacaaggag ttccatgaga catacatatc cattcatatt
    24541 ttaaattatc caattctctt atagatttca tcatcttttt ataatcccaa ttccatggat
    24601 taggataagg aactatattt ccattcttat ttttgactaa atcacctgca aataatacat
    24661 cttcaaaaat catgcagaca tgccctggag tatgaccagg agtatggata atttttatat
    24721 tcccaatctg catattctct ccataaggtt caacatcctt aatgagcctt ctggaaattg
    24781 cctttccaat aaacttctta aacccaggtc tatccttctc tcctataata aaaggtatat
    24841 cctctacatg agcccatact tttgctcctg ttttttcttt caataattta atattagata
    24901 tatggtcaac atcatgatga gttaataaaa tatgcttaat atctgtaagt ttaatgccaa
    24961 gtgtttttaa ttctttaatt atatttttcc ccataaaagg aagacctgtg tctataagaa
    25021 taatttcttt atcaaacaca ataaaacaat ttgagccacg agtactatct aacattaata
    25081 cattttcttt tatcttcata aataaattcc ccttatttgg tattttacct atataattaa
    25141 ttaataaagt tataaatagt tataatactt gatattaatt ttatttttta ttaaagtata
    25201 ctataggtat acaaatatta tatttacata aatataccat cagtatactt ttttatcaat
    25261 agaaaggaat aatttatatg tcagaattat caaaaagaga taaagaaaaa atacaaagag
    25321 aaaatgagat tattgataag gcagaaaaat tattttgtct taatggattt gataatacaa
    25381 ctatgaatga attagcaaaa gaagttgaat atactaaacg caccatatac aagtactttt
    25441 catgtaaaga agatttattc tttgctgttg tcttaagagg atataaaaga ctttgggata
    25501 atgttaaaat agaaagtgca aaaggcaaaa cagggtttga aaaaataaaa ctttcatact
    25561 ttgcttttca taaattttac tctgtagaac cttctctatt atgccttatg ggaatgattg
    25621 gtattgtaaa ctctgaaaaa tcggatactg atatgctgtt taaagaaaag tttttttctt
    25681 ttaataaatt tatgtttgat gaaatacaag gaatgtttga aattggaaaa aatgatagaa
    25741 gtattaggca tgatattgaa atacctactc ttatgtattc atcaatattt acacttacag
    25801 gattttttaa tttactatct gtaacaggaa aatcatacct gaacaacttt aatatagatg
    25861 aggaaaaatt tattgaaaca actcttgctc ttttaataga ttcaattaaa gcataaacct
    25921 aagtttataa attaaagttt atatacttaa atgataattt tttctatctc taatatgaaa
    25981 aatattaaac ataaaactaa taacttagtt aatggaataa ctaaattact gaaaggatta
    26041 aaagtaaatg gctaaaatac ttcttttaga agataatgat acaattacat ttggaataaa
    26101 aacttccctt gaaaagaaaa atcacactgt atgttgttat aatacgattg ctgaagctaa
    26161 aaaaaatttt gataaaacta ttgatttaat tctgcttgat ttaaatctac ctgatggtat
    26221 tggatacgag ttttgccaat atgttaaaag tatagaagat actccagtga tatttttgac
    26281 agtccgtgat gatgaaaaag acattatacg tggattagat atgggtgcag atgactatgt
    26341 ggttaaaccg tttttgctct cagtacttca atccagaatt aatgcagttc ttagaagaac
    26401 tcattcaaat actaacaata ctattacatg tgaaaatata agcttgaata aggttgaagt
    26461 caaagcatat ttagataatg ttgaagtacc tttgacagca ggagaatata aacttcttct
    26521 aagtcttatg gaaaataaaa atagagctat gactcgtaca aagttactag aaatgctctg
    26581 ggatgtagat ggtaatttcg taaatgataa cactcttact gttacaataa aacgtcttag
    26641 agaaaaatta aaaaatccac cttgtataaa aacattaaga ggcataggat atagaatgga
    26701 ggacaactag tgaaaactcc aagaaatatc aacagtacag ttgtttttat aatattctgc
    26761 ttaattgtaa cttttttatt gagtgtaatt ctaacaacta taacttcttt taatgaatac
    26821 aatgtactgt ctaaggctct tggtgcatca tatgagtaca atcctaagtc tacagaaagt
    26881 atactaaaat cactgaaaaa ttctagtgat aaggatttta aaacaggaaa tgaaatttta
    26941 tcaaaacatg gatattctaa aagtacattt tggagtaaaa acttttttta ttttctagtg
    27001 atatcgtttg gtataatttc aacaatggct acagttgtat atataatcaa acatagaaca
    27061 aataagcaaa aactgagtag aataaacaat cttactcaat acttagaaga agtgaattta
    27121 ggaaaagagt ctgtactttt acgatgtgaa gatgaatttt ctcaccttga agatgagata
    27181 tataaaacag ttggggaact tcgttgttca aaagaatttg ctttaaaaga aagacaaact
    27241 ctttcagata accttgcaga tattgctcat caacttaaga ctccaattac ctctatgtca
    27301 cttatggcac aattactttc agaaaattgt ccagatgaag aaatagatta tattaacaga
    27361 cttacaaatc aaatctcaag acttgagaga ctagtttcat ctttacttac attatcaaaa
    27421 ttggatgcaa gtactttaat atttgaaagc aaacttatag atgtacattc tcttcttact
    27481 tatgcaattg agcctataga aggttcttta agagaaaaac atcagacatt tactatcgag
    27541 gcttctcctt cggtagaatt tgaaatcgat attaattgga ctttagaagc tcttttaaat
    27601 attcttaaaa attgtagtga gcatatagaa gatggtggat atatagttgc ttattatagt
    27661 caaaatcctc tatatgttga gattatcata gaagataatg gtaaggggtt tgctcaggaa
    27721 gaacttccat atatttttaa tagattttat cgtggtaaaa atgctagtaa ggacagtatt
    27781 ggtatagggc ttgctctatc aaagtctatt atagaacgtc aaaatggtac tctacatgca
    27841 gaaaattgcc ataatggtgg tgcaagattc ataattaaat tttacacata aaatgctgga
    27901 ttataggtaa aatacctata atccagctat atctatattc tatgttatct attgttttag
    27961 taatatactt ctatttacat ttaaggtttt attaaacaaa caacttaact atccctatac
    28021 ttgtaagtag tcttaaatcg aaacctttaa ataagttcaa atagattttg tttatattgt
    28081 ttcatttttt atagattcta ctatattttc attcttcaac tgcttgtatc ctgtcatata
    28141 tgatgctatt acaataaaaa gtatcaatat agtaaaacct attattgtag atataggtgc
    28201 aaatggtaaa tactctataa aatatatttc atttatacgt agaaaagcat acactattcc
    28261 tatttgcaca ggaatactaa gtagtaatgg tgtaattcct aagaataaac cttccaaaat
    28321 aagcatcttt tttatgcctt ctggagataa tccaacagaa cgcaacatag caaactcttg
    28381 tcgtctactt ctaatatttc ctgacactgt agcaagtaca tttgataatc caatcatagc
    28441 caataaacct gtaataaagc ctactattaa attcatggtc tttattccac tagccttttg
    28501 tttttcactt tctaaaacat cctcaatact ataatctcct gaaccatagt ctttattata
    28561 aatggactct aatttttctc ttactctagg aatgtcctca cgattattta ctttaaatac
    28621 tcctatcagc ctatttgaac gtaacctact tacttcatca taatttttag atatttcctg
    28681 caccatagac attggaacta tttgcattaa tgtatacttt gaactagtat ctactatttt
    28741 aggaagtgtg cttgtaatag ctcccacttt catgttgtaa gtataatccc ctttatcttc
    28801 gtcatatact ttttcattta atttaatgtt atctcctatt gaaagattta aataatctat
    28861 atatacttta tttctacgtg tactacgatt aatatcttcc tccttattat atacaattga
    28921 ggttggattt tttgtatcat aaaataactt tgggtctaca gaaatttgcc tacaatattc
    28981 tgtaaaactc ttatcatcaa gcccaattat atttgaaatt atacgaaact tgccatcacg
    29041 ttctatagga gagtactttt tagatgaagc tatattttta aatcctccat ttttcgcaag
    29101 ttcttttgaa gcatcttttt cagtcaacca agtggaagca gacatttttt gataatatag
    29161 tgaacttttt acaccttctg tattatctaa tttttcaatg aaagcagact ctacaggttc
    29221 actattttcc aaataaactg ctatatctct ttggtcctta taattatcac ttccataaac
    29281 agcttttgct ccttcttgat ttgcaattat atgcaaaaaa catgtaagta ataaaaaaga
    29341 taatgttaaa gatattgttg cagtacgata agatttttta cgagcacgta gtgcgtttga
    29401 tgcaagttct ccttctattc caaatatctt tcctaatgaa tatttatgcg actttttgat
    29461 tttaacacta tctccttgtc gtattgcttc tataggagat atctttgata cttttcgtgc
    29521 tggtataagt gcagaaatcc agactgttac tattgtaagt aaaactgctg gcagaaaagc
    29581 aggaatacca taagtaaata ccacttctgg aacatcagta tgataattaa ctgaattaac
    29641 ataaactata agcctattac acaataacca tcctaaaaat aaacctattg gtaatggtat
    29701 aattgtcagt aaaaagcctt caaacactac agacctttta atttgttttg gtgatgcacc
    29761 aacactagat aatatcccta attgagtaag tctagcattt gcagacagtg aaaacgcgtt
    29821 gtaaatcacc attacaaaca cggctactgt aaaaattgct attgtaagat acataatagg
    29881 agttgaaaat tttgatatac tactcataga tgctttctgt tctggtgaaa agactaacat
    29941 ctttaaaaga taattactat tgtatttaag attgtatttt ccatattcat tagtcttcca
    30001 tcccaatgat ttagcaagtt ttggaagttc cttatacgtg tctcttatat ttttaaatcg
    30061 caagtacaca gttatactat cattaggttt tatacttgtt ttatcaagat atccaagagc
    30121 tgtatatgca ggtattactg atgaagtaga aacatctacc tttcctacta ttgtcaatgt
    30181 agcctctgaa atttttttaa aactttcccc tttcgatttt ggggctatgg gttcaagagg
    30241 ctttccattt aggattctat ctcctattgg gagagtaatt ttatcaccaa tttttacact
    30301 tggattattc tcaaaatatt gttttgataa tgcaatttca ttagcattat ttggtcgacg
    30361 accctctatt atagtatcct tttctggcat agaattccaa tactctgatg tagcatttct
    30421 ataaattata taatctcttt tctcatcatt aattttgcct acataccatt gtccttttac
    30481 cattacatca tcaactgaag aaaaattttc aattaatggt aagtcacttc cataagtata
    30541 gtcaaacaat tcaccatgcc agttaccttc tttctcaatt gaaagtttta taaaatctgt
    30601 ccacatggta tacaacagtc cacacaaaga agacatcatg gttgtagtaa gtaatatagc
    30661 taacattata gctatactac tacgtttatt gcgtcgtaca aagtctaagg tgtattcttg
    30721 aagaatattc atttcttcac caccttttca gagacaatca ctccatcttc catagttaca
    30781 atacgatttg cctctagtgc tattttctca tcatgagtaa taagaagtat tgtctgatta
    30841 aatcgtttat ttgataagtt taacaagtct acaatttctt ctgaattttt tctatcaaga
    30901 ttaccagttg gctcatctgc taataaaata gctggtctat agattaaact tctagctata
    30961 gatacccttt gttgttgtcc acctgaaagt tgacttggaa gatggtttaa tctatcagat
    31021 aatcccaata tagatacaat ctcagagaat ctatcctcat ccacctttct tttatcaagt
    31081 aacatgggta ataatatatt ttttctaaca tcaagggtag gtataagatt ataaaattga
    31141 taaataagac ctacttttct acgtctaaaa actgcaagag cttcttcctt taaacttgat
    31201 atttctgtat cttcaacata aatctttcca gatgtaggtc tatctactcc cccaagcaga
    31261 tgtaacaatg tagatttgcc agaaccagat gctcctacaa tggatacaaa atcacctttt
    31321 tgaactgata aatttacatt attaagggct gtcactctgg tttgatttga accatatatt
    31381 tttgtgagat tttcacattt taatatttcc atatacaatc atctccttta tggttaaatt
    31441 ttagcaacct aaaatgacat tatagtgaca tgaatacaaa tataaattac tattaaaaat
    31501 attgtttagt ttttgcacat tatttaaaat tataatataa aacaaaaac
//
LOCUS       DAYAOY010000045        31373 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYAOY010000045 DAYAOY010000000
VERSION     DAYAOY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.045"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(151..1179)
                     /locus_tag="ACGI6B_002106"
     CDS             complement(151..1179)
                     /locus_tag="ACGI6B_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9751549.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1227..2087)
                     /locus_tag="ACGI6B_002107"
     CDS             complement(1227..2087)
                     /locus_tag="ACGI6B_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFG9751550.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2356..2565
                     /locus_tag="ACGI6B_002108"
     CDS             2356..2565
                     /locus_tag="ACGI6B_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFG9751551.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2565..2831
                     /locus_tag="ACGI6B_002109"
     CDS             2565..2831
                     /locus_tag="ACGI6B_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFG9751552.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2860..3432
                     /locus_tag="ACGI6B_002110"
     CDS             2860..3432
                     /locus_tag="ACGI6B_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9751553.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3667..4857)
                     /locus_tag="ACGI6B_002111"
     CDS             complement(3667..4857)
                     /locus_tag="ACGI6B_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9751554.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5117..5548)
                     /gene="dut"
                     /locus_tag="ACGI6B_002112"
     CDS             complement(5117..5548)
                     /gene="dut"
                     /locus_tag="ACGI6B_002112"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFG9751555.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5661..7889
                     /locus_tag="ACGI6B_002113"
     CDS             5661..7889
                     /locus_tag="ACGI6B_002113"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFG9751556.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(8000..8485)
                     /locus_tag="ACGI6B_002114"
     CDS             complement(8000..8485)
                     /locus_tag="ACGI6B_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9751557.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8737..9960
                     /locus_tag="ACGI6B_002115"
     CDS             8737..9960
                     /locus_tag="ACGI6B_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFG9751558.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9964..13098
                     /locus_tag="ACGI6B_002116"
     CDS             9964..13098
                     /locus_tag="ACGI6B_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFG9751559.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            13120..14430
                     /locus_tag="ACGI6B_002117"
     CDS             13120..14430
                     /locus_tag="ACGI6B_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFG9751560.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14719..14964)
                     /locus_tag="ACGI6B_002118"
     CDS             complement(14719..14964)
                     /locus_tag="ACGI6B_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9751561.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15178..17805)
                     /gene="ppdK"
                     /locus_tag="ACGI6B_002119"
     CDS             complement(15178..17805)
                     /gene="ppdK"
                     /locus_tag="ACGI6B_002119"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HFG9751562.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17866..18708)
                     /locus_tag="ACGI6B_002120"
     CDS             complement(17866..18708)
                     /locus_tag="ACGI6B_002120"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFG9751563.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18715..19353)
                     /locus_tag="ACGI6B_002121"
     CDS             complement(18715..19353)
                     /locus_tag="ACGI6B_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751564.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19789..21081)
                     /locus_tag="ACGI6B_002122"
     CDS             complement(19789..21081)
                     /locus_tag="ACGI6B_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFG9751565.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(21118..21474)
                     /locus_tag="ACGI6B_002123"
     CDS             complement(21118..21474)
                     /locus_tag="ACGI6B_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFG9751566.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21509..21868)
                     /locus_tag="ACGI6B_002124"
     CDS             complement(21509..21868)
                     /locus_tag="ACGI6B_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFG9751567.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21937..23910)
                     /locus_tag="ACGI6B_002125"
     CDS             complement(21937..23910)
                     /locus_tag="ACGI6B_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751568.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(24132..25145)
                     /locus_tag="ACGI6B_002126"
     CDS             complement(24132..25145)
                     /locus_tag="ACGI6B_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFG9751569.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(25158..25703)
                     /locus_tag="ACGI6B_002127"
     CDS             complement(25158..25703)
                     /locus_tag="ACGI6B_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFG9751570.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            complement(26079..26948)
                     /locus_tag="ACGI6B_002128"
     CDS             complement(26079..26948)
                     /locus_tag="ACGI6B_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HFG9751571.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            complement(27100..28215)
                     /locus_tag="ACGI6B_002129"
     CDS             complement(27100..28215)
                     /locus_tag="ACGI6B_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFG9751572.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            complement(28451..29587)
                     /locus_tag="ACGI6B_002130"
     CDS             complement(28451..29587)
                     /locus_tag="ACGI6B_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9751573.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            complement(29643..31106)
                     /locus_tag="ACGI6B_002131"
     CDS             complement(29643..31106)
                     /locus_tag="ACGI6B_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9751574.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
BASE COUNT        10663 a         5307 c         3839 g        11564 t
ORIGIN      
        1 aaagcaatat acaataaaaa aatgtagaaa gtaagtatct ctctacattt tttattgtat
       61 attattttta tattaattta attttatctt gattcagttt tataaaaaat tgttataatt
      121 gtcaagttct actctattat ttataatttc ttatatctta ttaacaacat ttaggttatt
      181 acatttattt atatatattt tttcattatc taagtcttca tcaaacattt ttattatatt
      241 ttttatctca tacttgtact catcttcatt cattctataa agcattaaat cttctagtac
      301 atctttcatg tcgaagtaca catcttctaa taaataaaaa tagccttcct ctatatcttt
      361 caatttatct atagtatcca tttttttact tatatctttc ataatttcta aattttgtat
      421 atttacatct atgattgtta ataagtacat tctcacagtt tttattattt caacagaaaa
      481 atctacaata ttataatttc cttctttttt atataatgac tccttaaatt tgttaaaaca
      541 attatctaaa ttagaattta tatcgttaaa tggtggtctt attatgtcac gtattattaa
      601 aagattttgt atatctacat tatttgtgat tgcctcatcc aaaataaata aattatatgc
      661 atcatttaat gggtcatggg catctccact aaactctaag ttaacaaatt caagtaaatt
      721 tttcaatgat atatagttca tacatctcat accacaattt atatattttt cttttatatc
      781 tacaaataaa tctctaatgt tatttacaaa ccttggatgg ttattttttt tagcacttct
      841 catatctgtg ttattgaaac atgtcaaatc caaattacca aaagtatata ttcctttaat
      901 atcagaaaat atgccaagcc attttttaaa ctttctcatg acctcttcat aacttggtgc
      961 actcttcaat tcttcagaag atatatgtgt taattcttgt atatgaggat acaactcctc
     1021 attacttact ggtcttatta aagatttaaa tttatcaata ttttttgttt tttcatcata
     1081 cacaactgct ccaattgcaa ttatatcaga attaaatact gctctttttg atttagaatt
     1141 tggcatattc atttcaaaat ctatatatac tcttttcaat ataaacaccc tttccaattg
     1201 tttatagtga ttctttttaa ctatttttat tcaaaatttt ctaagaaaga atgtttctcc
     1261 ccatcttttt gccaaccaag tactgaatca agatttacat tttctgccgc cttaaatata
     1321 gatttttcta catttttaaa atttgattct aaactcttta ttaagtcttt tatctcatat
     1381 cttttattac ctgttttttt agcagcaacc atacatgcac aatttaatgg ccatatacca
     1441 ctattttgaa taaatcttat aatatgttct tctcttatat aataaagtgg tcttataatt
     1501 tttatacctt caaaattagt agaattaagt ttaggaagca ttgttttaaa atttccagca
     1561 cacaataagt taagcattgt tgtctctatt acatcgtcat aatgatgacc taatgcaagt
     1621 ttgttgcacc ctagttcttc tgcctttgaa tataatgctc ctctcctcat tcttgcacac
     1681 atatagcatg gataatcttt tgctatttca tctgctatct caaatatcct tgaatcaaat
     1741 aaatgtattg gtatatttag atactcacaa ttatctataa gtaattgtct tatatttgca
     1801 tgatatccag ggtccatagc aataaattca acatcaaaat taacttgtcc atgttttttt
     1861 aactcttgaa acattttagc cataagaata ctgtcttttc caccagaaat cgctacagct
     1921 atcttatcac cttcttcaac caatttataa tctcttattg ccttcataaa cttagaccat
     1981 aaattttttc tatatttttt tattatactt ttttctatat ctcttactgg aagtctttcc
     2041 tcaaaaggaa ctataatttc acagcctttt cctgatagtt cactcatcta atcacctcgt
     2101 atatttatgt taataatatt ttacaaaaaa gtctaccttc atccacaaaa tactataatt
     2161 tttactttaa ttttactaaa taatttatag tttacaataa cacataaact tataataaca
     2221 taaataacct ctttctttta ttatcttttt tatttttctc atttaatatt ttattattag
     2281 taacgatttt taaatgattg aatattatac actagataga gttacctatc tatttacaac
     2341 aaggaggttt tcatcttgga aaataagact agagatatca aaaaaaattg tggtccatct
     2401 ttggctagac catacattaa taatcaaatt ttcacagaaa tgtactgcct ttctgatgca
     2461 ttaaaatttg gaactatatt tccagaacta aacttactag agtctgaaat gtacaacaaa
     2521 gaattatatg ctaagccaaa aaaatataga ggaggtagaa gataatgaaa gactcatcta
     2581 gagctgaact tatgagaaaa gttcaggaaa catcttttgc atgtgttgat atgaacttat
     2641 atttagacaa tcaccctgat gataaaaatg ctatcaacac ttataattca ttatgtaatc
     2701 aatttgcgca agctagatat gcttacgaaa ataaatacgg tcctttaact aactttgggt
     2761 atgctccaag tagatgccct tggcaatggg ctgaccaacc ttggccttgg gacagagaat
     2821 ttaattatta gacatcaagt attggaggat tgaaaattca tgtggatata tcagaaaact
     2881 atacaacacc cagttaatat aaaaacttgt gaccctagaa tggctaaatt tcttataact
     2941 caatttggtg ggccaaatgg agaacttgct gcatctttaa gatatttaag ccaaagatat
     3001 acaatgccta ctggtaatat gcgtgcactt ttaacagata ttggtacaga agaactagct
     3061 cacgttgagc ttatatgtac tatggtttat cagttaactt ctgatgcaag cccagaagag
     3121 ttaaaagctg caggtcttgg ttcaaactat gctcaaaatg gatatggaat ttatccaaca
     3181 gattcaaatg gtgttccatt tgatgtaaga cctatagcag ttatgtcaaa tcccgtaacc
     3241 gatttacatg aggatatggc agctgaacaa aaagcacttg caacttatta tcaacttata
     3301 aacctaacag atgacgttga tgttatagat gtattaaaat tcttgggtca aagagaaata
     3361 attcactatc aaagatttgg tgaagcttta atggatgctt acgagttaga agaatctcaa
     3421 aaaatgttct aaaattttta tttgtataac tattattatt tgataattta taatttactg
     3481 ttgtgttaaa atttataaaa aatataaata taatttattt tctataaaca gagaaaataa
     3541 ataaaaagca ggtatcaatc aaatagtaac atgtattata ctatacatta ctatcaatat
     3601 tgtacctgct ttcatattat aagcaataaa gaaaaatcta tttattaggt tttataataa
     3661 aaacaattat aaaactcttc ttgcatttac atatctactt gaatagtagc tactatttat
     3721 attagatatt gttacttttt ttgatgaacc tgaagatgca tgtatcatat ctccatttcc
     3781 tatgtatatt ccaacatgac ttacagaacc attatttgaa ccttgtgtat caaagaatac
     3841 taaatctcca cttctaaggt ctcctctatt tacatatgtt ccatacttac tttggtctct
     3901 agatactctt ggtatactaa ctcctacaga ttttttcata acgtactgtg tgaatccaga
     3961 acagtcaaaa ctatttggac cttctgctcc ccatacatat ggttttccaa gtagtgtctt
     4021 tgcaaaactt attacactat cagctccttg acttggagaa ttagtcgaat aagatggaat
     4081 tgaagtatca acagaacctt tttcagcaag gtattgactt gacacatatc cagtttgatt
     4141 gccataactt acttttgtcc atcctccact ttcagaaata cttccaacct cagtaccata
     4201 acttaatgta gctattttag aatgacttgt acctggtcct gttctaacgt tcaatgactt
     4261 tgctgttaca acctttgtac cctttacaag gtcatttgaa ctgttttcag cattttcatt
     4321 tgtagagtca tttacactag ttcctaaata tttacttgaa acataaccta ctctaccatt
     4381 agaacttatt tttgaccatc catttgactc agatatgtat ccgacttcag tcccatatcc
     4441 tagtgttgat attttagaac tacttgtact tggtcctgtt ctaaaattta atccttttgc
     4501 tgttacaact tttgtcgact taactgaagt atcactactt tcattgctac ttccaaggct
     4561 atttattgct acataatttc catgcacata acctgtgttt ccattatatt taacttttac
     4621 ccaagaacct tcttttccta cgtactcaac tttatcacct ttgtaaagtt tccccattga
     4681 ttcatggttt gtacctggtc cttttctaaa atttaccgca ttccctgtta ttgttctgct
     4741 ttctatactc acactctcta cttcagactc attagcatat gctaaattcc ccatagtagg
     4801 cattattatc gcagttgcca ttatactggc tgctattctc ttttgattaa aattcataac
     4861 tttccactca tctcctcttt tctactatct ttatttcact tcaaaatata aaagaaataa
     4921 ttgacactat taaaatatca attatttctt ttatattaat attattacaa atttgtaact
     4981 atttgtctat agtaatattt gcttaccata aattaccatg tataaatttt aatctttaat
     5041 agcctgtaat ttagtaatat agccatcttt tacctttttt aaaaatggct atatttttta
     5101 acttttttgt aactttttaa aatccagttg agccaaaccc accttttcct ctttcggagt
     5161 cacttaattc ttttacttct tctatgttaa tatcatagat aggttttact atcatttgag
     5221 caattttcat atgcttttca accttaaaat catttttacc atgatttatt aatataattt
     5281 taagctctcc tctgtatcct tcgtcaatag ttcctggtgt atttaataca gtaacagaat
     5341 gctttaaggc aagcccactt ctaggtctta cctgcgcttc tgtcatagta ggaagctcta
     5401 tacatatccc agttttgata agtttagtct ctccaggagg tattacaacc tcttctatag
     5461 aatacaaatc cataccagca tcacctttgt gagcaaaatt aggtattata gcatcatcat
     5521 ttaatttttt tacttttaaa ttgtacatct tatatctcct ttcaactttc tataaatatt
     5581 ataacttaaa tttcgacctc ttgaagtttt ttattttttt gtggtaatat aattattagt
     5641 gttttaaagg aggatttttt atgaaacttg tacttgctga gaaaccatct gtggcaaaaa
     5701 ctatagcatc atttttaggt gcaaagacca gacaagatgg ttactttgaa ggaaatgatt
     5761 atataattac ttatgcagtt ggacacttag tatcattata tgatatgaag gattatgata
     5821 aagataaata ctctggctct tggaaaatga acaatttccc atttgttcct gaggataagt
     5881 ttaaatttaa gattgatagt tctaaaacta aacaattcaa cactgtaaaa aagcttttga
     5941 atagaaaaga tgtagaatat gtaataaatg ccactgacaa tgaccgagaa ggagaactta
     6001 tagcattttt aatattttta ctggcaaaaa ataaaaaacc agtaaaaaga atcttggtaa
     6061 atgaatggac accagaagat attacaagag gtataaaaaa cctaaaagat gaagatgaaa
     6121 tgagaaatct tcaagctgct ggatatacta ggcttattac tgactggttg attggaataa
     6181 atttcacttc tgttgctaca ttgaaatatg gaaatgggaa actattaaac ataggtaggg
     6241 taattcttcc aacagttaaa ttagtctatg atagagatat ggaaatacta aactttgttc
     6301 ctaaaaccta ttatgaaata gaagggcatt ttaaagctga agctggtgaa tataaaggta
     6361 aatatgttaa gggtaaagaa agtaaatttg atacacttga agatgcaaat aaaattatag
     6421 cttcaattac atctgaaact ggtaaaatac tagataaaaa agttactatg tcaaaggaat
     6481 atgcaccaaa actttttagt ttgacatcac ttcaaggata tataacttct aagtattcca
     6541 attttacatc tgataaggtt ttaaatgtct gtcaatctct ctatgagggt aatggtaagg
     6601 gtggatacat aacttaccct agaactgatt ccattttctt agaagaaagt ttggcttcaa
     6661 aagcttctca aactttagat aaattaaagg ttggtcttga atatgaaaat aaaattaaat
     6721 tttctaaaac taaaagagtt tttgattctt ctaaagtaga ctcccatagt gctataattc
     6781 caacttatat aattccaaaa agtttatcta aggatgaaca attagtttat gatgccataa
     6841 aagatagatt tgtagcaaac tttatgcctc ctgctgaata tgagaataca gaaattaaga
     6901 ctgaagtaga taattgtact ttcttaacaa aaggtaaggt gcttaaatca aaaggatacc
     6961 ttgaggtata taataaggaa gaaaaaaacg atttacttcc acttgtaaat aaagatgatg
     7021 ttgtagatgt gttagaaata aaaccattaa ctaagcaaac tactccacct aaaccatata
     7081 ctgaggacac acttttaaag gctatgaaaa attgtggtaa aaatgtgcct gaagaagata
     7141 caacagtgct atctggatat tcgattggaa catcagccac acgtgctgat gtgttaaaga
     7201 agataagtca agtgggctat gtaaagaaaa aaggtaaatc ttattctata acagaccttg
     7261 ggaaaaattt agttgaaatt tttcctgtta aagacttatt tgatgtagat tatactggaa
     7321 aacttgaaaa aagtttaagc gatattcaaa aaggaaagta tacaagaaaa gaatatctta
     7381 ctaatatcat tagttttata tatcaaaatg tcaatattat aaaacaagat gcttcaaaaa
     7441 atataaacac agaagcatat actttcgatt caaaaactaa aaaatttact aaaacaaaag
     7501 aaaaaaaaca gaaaaaagat gtaactaata ctgaaaaaga agttgctata tcaaaggatt
     7561 ctaataacaa aaaagaagtt aaaaatggtg atgaaaatca atctcttggc aaatgcccca
     7621 tttgtcaagg tgatgtacta gaatttgaca aaggctttgc ttgtaaaaat cataaagaat
     7681 gtaaatttgt tatttggaaa aatgataagt atttaagtct ttataaaaag aaagttaaca
     7741 aaacaatggt caaaaatatt ttaaaaaaag gtgaaactaa agtaaaatct ttaactgcta
     7801 aaaacggtaa taaatttgat gctacattaa aatatgataa aaatccagat actggctatt
     7861 tcaattggaa aatagagttt gacaactaaa tattcataag aaaagaaata gcagtagtat
     7921 aataatctat attatattac tgctatttct tttcttatga agttttaact tttttaatag
     7981 tgacttttac aatcacactc taacctaatt tctttactat ttctgataac tttaatgtat
     8041 gttcatctaa taaagttagt tcctcttcag aaagattaga taattttttt actagagatt
     8101 ctttgagaat agcttttact gcatcaaata atttaaatgc tttatctgtt aattcaaccc
     8161 taagaatcct tctatctttt gggtcaggaa atttattgac taaaccatgt tccactaatc
     8221 tgtcaataat cggagtcata tttgatttta aaattcctaa attagaagct atttgagaaa
     8281 ttgatgatga atttgatgtc ataagataaa gaattacctt agcatgtgat ggagccattg
     8341 gacattcttc aaaataagct ttcattatat cagagttatt ttgaaacttt tttaacaaat
     8401 cgctttcttt aaatatatta ttttcaagca caaataaaaa atctaaaaaa gagtccgcta
     8461 ttttaagtat attgttgtta tccatatttg taacatccct tcaatagata atataataaa
     8521 acattgttgt ttaatatagt ataaattata aactattaat aaatttaaaa caagaaaaag
     8581 ttattgactc ataataatta taaatttata atcaaattag agaattataa attgccaata
     8641 aagggaagtt ttttaattag tctaaaacta aacttaaatt ataagacact ttaaattcta
     8701 caacaagcta gtgtgcaata tataatagga ggagatatga aaagatattt aatagttttc
     8761 ttaatgtgta gttcactgtt tttagttgga tgtggaaaaa cagaagaatc tgaacctgag
     8821 aaaccaatag cagtttctgt tcaaaaagca ataggtggag aaattgaaaa cacaaattct
     8881 ttctcaggaa ctacaaaggt taaagatgaa acagctgtta ctgcacagac tgttggcaca
     8941 gttcaagaag tgtatgtaaa acttgggcaa aatgtaagaa aaggagatga attattgagt
     9001 ataagcagtc cagagctaga aaatagtgta aagcaaagta aagcctcact agatttagca
     9061 aaagcaagtt attcaagtgc cactggtggt tcattagagg cacaagtaaa tcaggcaaaa
     9121 acagctcttg acaatgcaaa gattcaatat gaggagtctc aaagaaatta tgacaataat
     9181 aagattcttt atgaacagga agtcattagt ttagaccaat ttaaaaaaat agaattttct
     9241 ttagagcaaa caaaacaaca gttagattct gctcaaagag catacgatac agcaacttca
     9301 aaaagtatac ctcaagctaa agcacttgct aaaaaacagc ttgaccaagc tcaagtatca
     9361 tataatttag caatgagtaa tttagacaaa ttaacattaa catctccagt agatggtaca
     9421 ataacagcta aaaactttga ttcaaaggaa atgataacac aatcacaacc agcttttatt
     9481 atttctaatc ctagtatact agaagttgat ttaaatgtag cagaatcaga tataggtaaa
     9541 tttaagaaag atggaaatgt agatgttata attgaagacc aaaggatttt aggaaaaata
     9601 gattatgtac cttcagtagt tgaccctcag acatctttat atcctgttaa agtacttgta
     9661 aataatacaa acaacaaatt taaggcaggt atgtctgctc aagtaaactt aagtgttgaa
     9721 aaagaaaatg gtgctgttac agtacctaaa aaagcaatat ttgaagaaaa tggtaaaaaa
     9781 tatgtataca tagcaacaaa agacaatata gcaaagaaac acttggttca aactggaatt
     9841 gtaacagagg ataaaataga aattaaaagt ggtgtaagtg ataaagatac agtgattatt
     9901 ggagggataa gtcttatttc tgacggaact aagatatttc ctgtagaaaa ggagaagtag
     9961 actatgaatt taacacagac ttccgttaaa cgtcctctca caatcataat ggtcttttta
    10021 gtattaatag tttttggtgg tattggatat aaaaaaatgt caataaatct aatgcctgat
    10081 atagaaatac cagttgtaat ggtcatgact acttggacag gtgctggtcc tcaagacgtt
    10141 gatgaacaag ttagtcaaaa agttgatgaa agtttatctg ccgtttcaaa tgtaaaatct
    10201 actatatcaa gttcacaaga aagtgtttct atggtagttg ctcagtttga atttggaact
    10261 aatttggatg aaataatgaa tgatgttagg tctaaagttg atgcattaca gaccagcctc
    10321 ccagatgatg cagctaaacc aacagtttta aagctggata tgaatgcaca ggccataggt
    10381 caacttgtca tatctggagg aaatgaaaat agttcacaag ccttaagaaa atacgctgaa
    10441 gatgtaattc agcctaaaat agaatccata gatggtgtta cttcagcaga ccttaaaggt
    10501 ggaaaaaaag cacaggttaa tgtaatagct gacccagctg ttctttcaaa ttatggggtt
    10561 agtttatcca ctataaaagg tgtcttatcc tctagtaata aaacattccc ttatggttca
    10621 attactcaag gtgaggataa aatagtttta agagcgattg ataaattaga atccctagat
    10681 gatataaaac agattcaaat tcctgtaaaa ggaggaaata cagttagatt agataatctt
    10741 tgtagtgtag attatggata tgcagacaaa gatagtattt atagatacaa tggaaaagac
    10801 agtttggtaa tagatatttc taaacaacaa gatgccaaca ctgtaaaagt tatgcagagt
    10861 gcatacaaat acgtagatga attgaataag gaaaatcctc aatttaaaat taaaattgct
    10921 aatgatacaa gttcatacat aagtgaatca ataaacagtg taatgagcaa cttgcttata
    10981 agtgctgtta tcgcatttat tgtaatattt gctttcctaa aaagtgtaag agcttcacta
    11041 gttgttgcag ttgcaatacc aatttctata ataggtgcta tagcaattct ctactttact
    11101 ggtgaaagtt taaatcttat tactgcaagt tcattggtaa tttcagttgg tatggttgtt
    11161 gataatgcta cagttgtaat tgaaaatata tttaaatata ggaaaagtgg cagattgagt
    11221 cttgatgatt gtgctattga gggaacaaga actgtaacaa atgcaatact tgcatcaaca
    11281 ttgaccacaa tagcaatatt cttacctatc atgtttacag aaggtatagc taaaataact
    11341 tttggggcat taggtaaaac actaatagct tcactagcat tctcctttat aggtgcaatt
    11401 acactcgttc ctagtgtatt tgcaaaatta agcagaggaa aaaatagtca aaagatgcaa
    11461 gaaaaagata gtcctatatt tgataaggta agtgaagcat ataaaaaatt attaagtgta
    11521 tgtttgagac atcaacgctt agttgtaata tcaagtttac ttatttttgt agcttcctta
    11581 tttggaatgg gatttatagg tatggacttt atgcctgcag cagacaaggg tgaattatct
    11641 atttctatag aacttccaaa aggtcttagc ctagaatcca atgattacta tgtatctatg
    11701 actgagaaaa aagtctcaga tattccagaa attaaaactt taataacaac tttgacatca
    11761 gattcaaata atagtaaagc aagtatagca attgaattag aatctcaaaa aaatagaaaa
    11821 agaactactg atgaaataga aaaagagata attgatagag tggctactgt accagattgt
    11881 aaaataaatg taagtcaaaa cagtagtgtt atgggtggag ctggaggagc agacttcaca
    11941 ttagaaatga aaggtcctga tttagatgta ttaaaaatac tgggaaaaga agctgaaaaa
    12001 gaatttagct ctataaaagg atttagaaat gttgaaactt ctcttacaga tacaacacaa
    12061 gaagctcaat ttactataga taaacataag gctcaagaat atggtgtaaa taccgctgaa
    12121 attgcaggca tgttacgtac agcaataaat ggtgactccg taaccactgc tactattgat
    12181 gattacaaag tggatgtaaa cctaaaattt aaagcagata ctattgatag cttagaagat
    12241 ataaagcaac taaaagtaaa ctctgcaact ggaaaagaag ttcctcttgg tgctttttca
    12301 gatataaaaa tggcagatgg tctacaatct atatctagga ctgatggaga tttttctgta
    12361 aatataactg ctacacttga caatttagat gctggtactg ctacaaaact agcactagaa
    12421 aaaacgaata aaatggtaat tcctaaagat tactctatag gtgttggtgg tgaaacagaa
    12481 atgatgaatg aatctatggc aagtcttgga ttttcaatgg tcatagctat agcattagtt
    12541 tatatggtaa tggtagctca gtttgaatca tttagtaaac catttataat catgttctgt
    12601 gttccatttg cattcgttgg agttgtgctt tcgcttctag ttagtggtca aagtttaagt
    12661 actgtcggta tgctaggtat aatcttactt atcggtattg ttgttaataa tggtatagtt
    12721 cttattgact acatagagca acttagaaaa acaaatcgta acaatgattt aattggtttg
    12781 gtttcaattg gttgtgcagc aagattaaga ccagtcttaa tgactacagc aacaacaatt
    12841 ttaggtatgt tgccaacagc actatctctt ggtgaaggtg gagaaactat gcaaccacta
    12901 gccatagtta taatgggtgg tctaactgta tctacactta taactttggt gcttatacct
    12961 actatatact taatatttga taaatgggaa aataagtttt cagacaaaat gcctaaaata
    13021 tttggtaaga taacagataa aataggaagt ataaaacgta aaataataaa gaaaccaaaa
    13081 ttgaaagcta aagtttaaat agaatggagg gtgattgtat tgaataaaaa aatgagcaaa
    13141 atagttgcaa ttggctgtgg tgttggtata ataactgcca atttaatccc agtttacgca
    13201 gataataata cttcttctat aaacaaagtt gataattcta ttttaagtga ctcaaataaa
    13261 agaacatctg aaaaggaaat tttgactgct agagaagctg ttagtgctgc acttaaaaat
    13321 agtgaaaaat taaaaatgaa atcagaagaa ataaaaatgt taaaagaaaa gttagaagta
    13381 caagatgagt ttgatagttt tactggaagt gacaactcgt ttccatacga ccaaattgga
    13441 cttcttaaaa atcaaagtga acaggctaaa ggctttatgg aggaccaaat agcaaatgat
    13501 attactaata aatttaacga tttagtttca agagaaaatg agctagacaa gataaaaaac
    13561 aatcttgaaa taaaaactaa agaaattaaa gatatgaaat taaaaaaaga tttagggtta
    13621 gtaacatctt tagaaactga atcagctgaa cttgaacttc aaacactaca aaatactcaa
    13681 aaagctaaac ttcaagaact aaaaaacaat caagactact ttaagttgtt gactaatata
    13741 gatttaaata attatcaatt ggataaagaa tatagatttg aatcttttag agtaggtggc
    13801 tcagttgatt catatatgga aggtaaggtt aatgaatatt taaaatacga ccaattgatt
    13861 ttagaacgta cagaagaaag ctttaatgat aaagatgaga acaaagcaga tttaccagat
    13921 agacctaatc ttacaagacc agttgctcct acaaaaccag agcaaggtaa cctaactgat
    13981 gatgaatata aggttcttac ggataagtat gaaaaagact acaagagata tgaaaaagaa
    14041 gttgaagcat acaatcttga aagacagact tatgctgctg gccttactac ttatgcaaat
    14101 tatcttcaac aaaaatttaa taccgaaaat ggtctagtaa cattagaaga cagtaaaaaa
    14161 gctcttaaaa aagggttaat tgatagctat gctcaattac ttgctttaga agatacaatt
    14221 cagattacta aaaaacaact tgatttatca gaaaaacaat taaaaaatac taaattaaga
    14281 tatgatttag gattaattac tctaactgat tataaaaaac aagtggtaag taatgaagat
    14341 gctaaaaata gctatgacac tcttattgtg aattataact cactaaaaaa tggtatagaa
    14401 aaaccttgga tacttaatac tggaaaataa ttctggtaaa tagaaagatt actttaacat
    14461 acgtttgatt aaaaaatagt tatgcacact cgtggcttta gtcatggaag attcagaagg
    14521 caaataatag aaatagatta gtaaaaatat atttgcttat aaaactgtat aattttatag
    14581 aggctatctc aaaatagaga taagcctctt ttttattgtt taaattataa aaatatattt
    14641 aattccatac aaaaagagtg ccttatgaac taaaaagttc attttgagac accctcaaaa
    14701 gcttaactct aaatttaatt attttttatc aaatgtctca tctattttac atctcaccat
    14761 gttaacaaat tcagaaatat tatatcctct aagtttgcat ccacatttag gacaataatc
    14821 caatttccta gataccaaag ccctacaact aggttctgga catcttattt ttagactctc
    14881 atctgctact ttaaatccac atcgtgggca tatactacct ggacgtgatg atacttcctt
    14941 tccacattta ggacactttg tcaactttat cccctccagt aaactcaatt taattgatag
    15001 ttgaaatttc tctctacaat aatatatgca atataattaa atacgtttca cttacaacta
    15061 tataagttaa atttttataa taaaaatccc ctatattcaa tttacaaata cttaatttga
    15121 tacattgaac aataggggat tttattttta tttagttaca tattttgcag tctattatta
    15181 tctaggattt tttatagatg cttgagctgc tgcaagtcta gcaattggca ctctaaatgg
    15241 tgaacaagat acatagttaa gtcctacact ataacagaac tctactgaag atggttctcc
    15301 tccatgctct ccacatattc ctagttttaa ttctggttta acttctctac ctaatttagc
    15361 tcccatctta actaacttac caacaccatt ttggtctagt acttggaatg ggtctttttc
    15421 taatatttct tcatctacat attgacctaa gaatttacca gcatcatctc ttgaatatcc
    15481 aaatgccatt tgagttaagt cattagtacc aaaactgaag aagtctgctt cttgtgctat
    15541 ttcatcagca gttaaacaag ctctaggtat ttctatcata gtacctacag tataaggaac
    15601 ttctatacct tcttctttta ttatagcgtc aacagtttct actatcattt ttcttattat
    15661 ttttaactca tttacttctc caactaatgg aaccattatc tctggagaaa ctttaacacc
    15721 tgctttgatt gcttctatag caccttgtat tatagcttta gattgcatta cacatatttc
    15781 aggataagtt attgcaagtc tacaacctct gtgtccaagc attgggttaa actcatctaa
    15841 atcaactatt ctcttcttaa tatcacttac ttttattccc atagattttg aaagttcttc
    15901 tatagtctca tcatcatgag gcaaaaattc atgtagtggt gggtctagta atcttatatt
    15961 aactggtttt ccatccataa ctttaaataa ctctacaaaa tcttctcttt gcattggtaa
    16021 tattttttca agagcttttt ctctttgttc tactgtatta gataatatca tttctctaac
    16081 tgctggcaat ctagcttcat caaagaacat atgctcagtt ctacatagac ctataccttc
    16141 tgcaccaaac tctatagctg cacgagcatc tctaggatta tctgcattag ttctaaccat
    16201 catacatttg tgtttgtcta cccagttcat taatttttca aaatttccag ttaaagatac
    16261 ttctgttttc ttaacatctc ctaggtatac atttcctgta gaaccatcta tagaaatgta
    16321 ttctccttct tttataacta catcatcaag tgctcttatt tctttattaa actcatcaac
    16381 ttttatttct ccacatccag caacacaaca tttacccata cctctagcaa caactgctgc
    16441 atgtgatgtc attccacctc tagctgtaag gattccttct gctttaacca ttccttctat
    16501 atcttctgga gatgtctcta gtctaactaa aactactttt tctccttttt catttgcttt
    16561 tactacatca tcagcattga aataaacttt accacttgct gcacctggtg atgctggtaa
    16621 accttttgct ataaccttag cttcttttaa tgccttatct tcaaattttg gatgtaataa
    16681 ttggtctaat tgattaggct caattctcat gatagcttct ttttcatcaa ttattccagc
    16741 ttcaactaaa tcaaccgcta catttattgc tgctgctgct gttctcttac catttctagt
    16801 ttgtagtata tataatcttt cattttctat agtaaactct atatcttgca tatctttata
    16861 atgaccttca agtatatgag taatttttac aaattcatcg aatacagctg gcattacatc
    16921 tgcaagagtt gatatatttt gaggagttct tatacctgca acaacgtctt caccttgtgc
    16981 attcattaaa aattcaccaa ataatttatt ttctcctgta gctggatttc tagtaaatgc
    17041 aacacctgta ccactagttt ctcccatatt accaaatacc attgattgaa tatttactgc
    17101 tgttcctaag ttatgagcta tatcattcaa tttacggtat acgattgcac gaggattatt
    17161 ccatgatcta aatacagcct ctattgcaag cattaattgc tcttttgggt cttgtgggaa
    17221 atcatttttt atttcttttt tataaattgc tttaaattct tttactattt cttttaaatc
    17281 atcagttgta agttctgtat caactgtgta tccctttgct tcttttactc tgtctaatac
    17341 attttcaaat ttatattttg gaacttccat agcaacatca gaaaacattt gtatgaatct
    17401 tctgtaacta tcatatgcaa atctttcatt ttgtgtagcc tctgctaagc caacaacact
    17461 attgtcatta agacctaggt taagaattgt atccatcatt cctggcatag agaatactgc
    17521 accagaacga actgatacta ataatggatt cttattgcat cctaatgttt tatttaagtc
    17581 tttttctaaa gtagctaaat gctcttctat ttcttttatt atctctgctc ttatactttc
    17641 attgttaaca taataatcat tacatgcttc tgttgttata gtgaatccag gagggacagg
    17701 taagcctatc ttagtcattt ctgctaaatt ggcaccttta cctcctagta aagatttcat
    17761 gtctttgctt ccttcaccaa aactataaac atacttagtt tccattgtta aatcccccat
    17821 taatcttttt attttaattt atatcatttt tattaataga cattattaaa tttctatttc
    17881 tttgtatatc tttagaccat tttctttcat tatgtctaaa attatacctg ctgtttcttc
    17941 tattgcctta tttgaaacgt ttattactgg gcatccaatt cttttcatta tctcttctga
    18001 ataatctaac tcctgaagta ttctttctaa attagcatag tttgctttac tggacaatcc
    18061 taaagctttt aatctttggg ttcttatctc attcagcttt tcaggtgaat tagtaagacc
    18121 tattatcttt ttagtatcta tctcaaacac ttctttagga attggtattt ctggaactaa
    18181 tggaacatta gctactttta tatttttatt agctaaatac atactcaaag gagttttaga
    18241 tgttcttgat attcccacta aaattatgtc tgcttgaaga acacctctag ggtcttttcc
    18301 atcatcatat ttcactgcaa attctatagc ttcaactctc ttaaagtaag attcatctaa
    18361 ttttctaatt ataccagctt ctcttttagg ttttcttcct gttctcttgc ttatttctct
    18421 tagtatatct gtcattaaat ctatacaact aagaccttct ctttcgcaat atctttcaac
    18481 aatactgcat aattcttcat caactaatgt atatacaatt aaagcgtttt cagcttttgc
    18541 attctccaaa gtttcaagta gtactccttt attaatcatg tatggaaatc tttttatttc
    18601 ataagtttca ttcacataaa actgagacat acatgccttt gcaacttgtt gtgcagtctc
    18661 ccctactgaa tctgatactg catatataat taggtttttc accagcatac ctccttaatt
    18721 gtcaactatt tctaagaata gcttagttat gttggttttt gatattctcc caacaactct
    18781 catatgattt ttgtcttctc ccttatattc aacaatcgga atcgaatcta cttcatgttc
    18841 tattattttt cttgaagcta ataaaacact tgctccttta gttaaagtga ctacatttgg
    18901 agttcttgtc attatcatac ctattggcat tttatttata tcagaacctc ctatagttgc
    18961 ttttagcaag tcttttctgg aaacaactcc acataactct tcattctcat ctataataac
    19021 tatactccct acatcagata aaaacatagt gactattaca tcatatatac tttcatcctt
    19081 cttagctagt acaggcatgc tcattatatc ctctactgtt ttttctttta cacttttacc
    19141 aattaaattt atttcactaa ctcctgaata aaaatatcca acttttggtc ttgcgtctaa
    19201 tattcctgtc attgttaata tagctaaatc agatctaagt gtagcacgag tcacattcaa
    19261 gctggaggca atactttcac tcgttattgg ttcattttcc ttaactatat ctataatttt
    19321 tagttgtctt tcattaagct gaataataat caccttcttt cattgtgcta tactttttat
    19381 cttttcgcat cttatatagt atattatata actatatata ccattctgta aacagatttt
    19441 ttattgaata cgatatattt tttactaagt tctctcgctt aaagatattt attatatatt
    19501 aatgggggat atttctttaa ttaatagaac tatattagta ttacagttaa tattataata
    19561 cataattggt cattttaatt cattttatat taaaattttt aataatatta tttcaatgtg
    19621 aatataaaat tgcagaacta attcaaaata atttttacga ctacaaataa cctataatat
    19681 acttttaaag aaagtatcct aacaagaata tataaaaatt taaaagggtt gatttaaaaa
    19741 taaccatcaa ccctttttac taatatctat ttatattacc atcaaatctt acccaaatat
    19801 tttttcttgc aattgtctta aatgatataa agtagtatct ttttctatct ctaccatatc
    19861 gtaagttata acttcacctt ttttaatatc ttttacaact ttagtctttt tacttatcaa
    19921 accaataggt acagcattca tagcctttgc tacatcgtat ttttctatag taccatagca
    19981 tgtatatgct ccatagccat ccataaactc accttttttc aaatctttct tagctatagt
    20041 cataacttct gctactagtc cagctttagg tactattgta gccttatggt ctaaaactgc
    20101 catagctgct gataaaggag tctctaaact acataaatga tatggtctat ataatatgta
    20161 attaggtcca tcacccatac ttaaatattt tagttcatca ttaactgctt ttaatttatg
    20221 ggcaactact acgaaaactc caggtgctat accatttaca aaatcaacta caccataatt
    20281 atctaatact ccaccttcgc tttttaatgc atatttctta ggtacttcat taactgtagc
    20341 ttcaatacca tgaccacctc ttacatcagg cacaaatcca gttgcattac acattaaagc
    20401 catttctacc attgttttag ttccttcttt aaaagaagct atcatatgag gtgaagctcc
    20461 ttttctttct gcttcttctc taactgtatc aggattacaa tctaaatcta atttattatt
    20521 tttaccttta cctatcactc taacttcaaa ccccattgca tctgcaaaat caaataattc
    20581 cattactgca cctggctcat ctcctgcaga acctgtaaat attactccag catcatctgc
    20641 taatttttta agtattggtc caacaacaca atcagtttca gcatttaaca taactatatg
    20701 ttttttattt aaaatagagt ttaatgctat ctctgcacca attgcaatac ccccagttgc
    20761 atctataact acatctattg acttagtttt acaagccaca agaaagtcat tacatgtcaa
    20821 tacttttcct tcttgtaatt ttatttctgc atccttttca tttgttacat tatctataat
    20881 ttcttcatct attcctgcat cagtaaaagc tttatgagcc aactccaaat ctatatctac
    20941 aacaatagaa gcttcaatac ctttcatatt tgccatttgg cttaccattc catttcccat
    21001 atgcccagct cctataagag ctacttttat ttttttacct tcctcttcca attttgcaag
    21061 ttttctgttt aaatctaaca taccaattcc tcctgttata ataattttat tttcaaatta
    21121 agaaatagtt attaaatctc caatagttat atctggattt cctatttcca catgtatttc
    21181 tcctggtaaa tttacttcac attttccatc aaatttgatt gtacaatgcc caatttctct
    21241 aagagtcttc tgagcaatat caccaactga aactatacaa taatctctat tccctatagt
    21301 aagtgtatct cctatttcaa catcactttt taatgttgct attgaatgaa ttacagatat
    21361 atcctttaag tcgtcatcat taatggtttc atcatatata ataagacaat tgctttcatc
    21421 cattaactct ttaactaaac tgcctatccc acttatctta acttcatatt tcatgacatt
    21481 cctcctgata atttatattt atattgagtt aattaaaaga aacatttatt ttttctttta
    21541 taaattttat agcttgtaca atactctctt catctttaaa tttttgctct gcactatata
    21601 tatctaatcc ttctatttct tcaatctctt taaatttagc cattactgta cgccctttta
    21661 aaatttctcc aaactttata aacccatctg aatctgaaga tataacggct atacttcctt
    21721 tagaaaaata gcttttcttt ttgcctgtag acactatatc accctttttt ttagctctat
    21781 ttaaagtttt aaaatacaat ttattctgct tcattataaa tatagtattt aaaatataag
    21841 caacaaataa aattactaat aatttcatat ttatacacct tccaaaaatc acattatttt
    21901 taaatctgta taacaactat aaatgctgta caatagttat ataaggtttt taagtaacac
    21961 ctttttaaat tctaaaaaag ttctacattt acttatctta ttcgttaatt ttacatcttg
    22021 gcctatcttt aaaacttctt cataaatttt tactaataag tctgagtcta tcttattctc
    22081 acatggaaac attgtcataa gaattatttt tatacttcta tttgcatatt ctattggttc
    22141 ttctaaaatt ccaactgcca ttaaaaactt atctgatttt tcatttatag aatgaggaaa
    22201 taataaacct ttatcaaata ctgtgggaga tttttcttct ctattgacta tattttttct
    22261 aaacttctca tcaatacatc ccaaatccat aagatggtcc atcatttttt ctaatgaggc
    22321 aagtatagtc ttttcattta atatcataaa tttatcttca tctaacaaat tatttattag
    22381 taaatttgta gaattagtag tgtcaatatt cccttctctt aggcacaata ctgtttttaa
    22441 ttgttctctt agtttgtttt catcaaatat tgtatttatt tttaatacta ttgtatttat
    22501 atttgatgtg tctaaatcaa tagttgtaaa aactatatcg tattcatcta agttaatatt
    22561 tctcaaatta aaacttgaaa gagtatcaat tttaggatta tttcctatca gtttttctac
    22621 tcttattttc aatagttttg ctgttcctag acctgtacta catataattg ctattttttt
    22681 tattgaatag aatctactgc tatttctttc taaatatcca ctgaaatgga ttgctatata
    22741 tccaatctca tcttctgaaa cttctaagtt atactttttc tctattactt ttcctgctat
    22801 ttgtgctaat ttaaatggaa gtatataatt ttgcttaatc tcttctaaca aaacattttt
    22861 taactttata ttaaataata gcctgtttaa agtaaaacta agatgatatc ctagacctac
    22921 tcttaattca tcctcattta taaaaatacc catatcgaca tatatttgat taaatatatt
    22981 ttccatcaac tcatcaatgt ttttactaat tctactacta tttaaagcac attctgatgc
    23041 tggtgcattt ccactaacta acggtatagt taaataaata atttcttcat catttaaagt
    23101 acattcaaac attcctgata ttatatgttt tatttcccta gcaataaagt attcatcata
    23161 tgatttaagt aaatcaaact ttacatcata atcttttact ttcttttctt cttttactct
    23221 atagactaaa actactatgt agttaataac ttctcttaac atttcatctg taacataaaa
    23281 attatttttt ttaaataaag tcaatagttt ttcttctaaa tttatgattt cttctttttt
    23341 gattccttta aaatactttg aattactgaa atctttcctt gagtttttat acaactgatt
    23401 taatataaac agtctaatat caagttcatt tccatttaat ttcatacctg tattctgttt
    23461 tttaatcaag tttaaattat atgaagccaa aactttttct acatactgga aatcatttac
    23521 taaagtagtt cttcctacac acatctcatc agctaaacta tcaagtgtaa taaatccatc
    23581 tagttctatc aagtaattta gtataaactc tactctatct tcttttgaat ttaatgaaaa
    23641 tacatttttt ctattcatat caataatctt attgagtatc tgctcatctt ttatttctag
    23701 tttatagcct tgacccttta tatatttgat tactgctata tcattaccta aatctttatt
    23761 caattgtttt atatacgtcc ttacacttct agttgtgaca ttcaattcat ttgctattga
    23821 gttaatactc acaaactctt ttttactaca cataatcaat atcttaaata tcttgcggtc
    23881 aatagaagga attttctcag ttacactcat tttgtatctc ctccttgatt tcattctagt
    23941 acaaatttga aaccaaatta atacataaaa tttcacttta ataaaggaaa atttagtatt
    24001 aatctaactc gataattaca gctaattaat agtataaaag ctagtctttc attaacaaaa
    24061 aatcttgact agcttaaatt tatatttaca attttattaa ttaattccaa gccttatatt
    24121 gacattaaat attatctcaa tgcaaatact gcaactccat atgcaataat aacagctatt
    24181 ggaccagtta ttaaacgaga gtaaaataaa gctggaacac catactctac agtttctggt
    24241 tctgcttctc ccaaacttaa tcctactggt acaaaatcac atccagcttg cccatttatt
    24301 gcaaataaag ctggaagtgc taaatatgct ggtattgcac caagaccaat ttgtgtacct
    24361 acaagagtcc ctactacttg agctataact gcaccaggtc ctagtattgg tgataagaat
    24421 ggaagtgtgc aaattactga tataatcaat aatccaccaa tattacctgc aagtggagat
    24481 ataactcttg caatccagtc tcccagtcca gatgctaaaa taattcccat aagcatactt
    24541 acaaatgcca taaatggtat tacgttattc attaccattt ggatagtatc tcgacctgca
    24601 tcataaaatt ttgatactac ttgccctgcc ccttgaccaa tagaaagtat tatgttcttt
    24661 ttagcatatt caccttgtgc ttttgactta acctcatcat aattatcagg ctttctaata
    24721 gaactcttat tttcgctttt attttcttga gatttttgtg gcatatcttc cccatctaca
    24781 acacttatac agttaggatt aacatctgat acataatatt cttctgttat aaattttgct
    24841 aaaggacctg tttttccaac tggatttaca tttattgtag gtattttttt ctttggataa
    24901 actccacatc tagcagttcc accacaatca ataataacta ctgctacttc ttcatcagaa
    24961 actccagttt taaatccatc tacaatttca caacccagta gttcttttat ttttaatgtt
    25021 acttcatgga taccgccagc agtcattgat agtatcttat ttctattccc ttcttttttt
    25081 atatacaaag gacctcccca gcctgaagtt cccttttcta tttttaatat tttattctcc
    25141 tccattttat ctacctccta agccttattt gttgctctat tttttcttgt tagaaaagca
    25201 tacatttttt ctgttactat acctcttata aaaattacaa ttagaccaac tataaaatac
    25261 cacaatgcca atgtagtagt ttctttacct aaagtagtca aacctgcaga tattcccaac
    25321 catacaaaaa gttctcctgc atttgcatgt ggaaaaagac ctgtaattgg atgtacaaat
    25381 gaaactgctg catcataaaa tcctgctttg tgctcttctt ttaaaaatct accaaatgta
    25441 tagcacatag gatttcccaa gaaaaatact gatactaatg gaagtagaat atatcttgta
    25501 aaagagttct ttcctagctt agaagcccaa ttattaactc tttcctcacc aattatctta
    25561 atcaaagagt taatagctac cattaataca attagcgttg gaagagtacc tgaaacaaga
    25621 cctataaatt gttctccccc tttctgaaac aaaccgataa accactctgc acctttactt
    25681 agacctgtaa taacgttttc cataaaatta taacctccct ataatatctt taaattttta
    25741 attttactgc ttaccataaa ttttacaaat tacatctttt ttctgtacta tatgctaata
    25801 ttgtaaatgt ttttttatta atatcatttt aatataatat ttttgataaa taaatacatc
    25861 ttttttcaat tttataaagg aaaaaattgt actaattaga attttcagaa atatacaatt
    25921 cttatttcga ctatattcta aattcttctt agaaatttaa gaacttattt ttatttcttt
    25981 tattattaaa attatctaaa tcttataaaa ataaaaaata gatgctcttt tacgaacatc
    26041 tatttcttaa tcaaattatc ttttagtttt cttatagatt attttatagg tatataaata
    26101 tcaatttgtg agttcacatt actaggacct aaaaatcgtt catcatatac ttcaaaatca
    26161 tctcttatat caagttcttc ttttgaactt aaaaaccaag taccccatat atattcataa
    26221 gttttgtcta aattatctaa gctaccagta tgagtaaata tagcatactt tccttcctta
    26281 atagtttttg aaacaaatga atttggaatc acatcataac tattaacttc aagtcctatt
    26341 atctcattaa actccatact ttcactaact aggtgtattt cagagattgc ttcacagatt
    26401 ccaaaaactc tccttgatgg caaagtattt gggacaatat gatgtacttt tctgaatttc
    26461 tcccacaagt caggtaatga attattttct aaaattactt tatcctttat acctattacc
    26521 tttatttttt ctatttcttt tataactggt ttaatcgtta tatttccaac aagatgtctc
    26581 ataaaatcta actctaattt tttcttttta ccaacaaata catcaattct attttttcta
    26641 tattctatag ggctaacctt gtatatagat ttaaatgctc tgctaaatgc ttctgaagat
    26701 tcaaatccac taccaatagc tatatctatc acttttttat cactatataa aagttctttt
    26761 gtactactta ctaatcgtct tttttttata taacttccta cactttcacc aaacatagac
    26821 ttaaataagc gtgttaaatg ataataagaa tatccagttt cttttgccac atcatttaca
    26881 gttatatttt catgtaaatt attctcaata tacattacag ctttttcaaa atactttaag
    26941 taatccaata tggcacctct cttctactta aactctttaa atatgtaaaa tagcactatt
    27001 ttttactata ctaaaatatt ataactaatt caatacgata aataaaaact tttagttgat
    27061 ttatatgtcc taattattta gaataattaa atacattttt tattcaatga aatcaaaaaa
    27121 gtcagatggt gctttttcaa tatttatgtt tatcctctgt gttatagatt taatgtcatt
    27181 ttttaaatag tagctctccc atttattctc tagtccaccc cataaaatat taaactttcc
    27241 tatatttaaa tccataacta tactttttaa agttccaaaa aaatcattat agtaattaca
    27301 agataagcca tttggatatt ttgaagacaa taaattcatt aactctttct ctccaatttt
    27361 tttagactta tttatatatt ctttaattaa tttatatcta tgaatagaat ttttcattga
    27421 ttgtggatct aatttatgaa gtttatcaat atgtgtatga ttagtagagt gtaaaaaata
    27481 ttctctttta ctttctaaac tgttgattat atttacttct tttttcccat ctaaagtctc
    27541 cacaagtgca atgtttccag acttatctgc aagcaataaa ttaatattgt atgcaattgg
    27601 catatctttt aaatattcta atgcttcatc tacatctttg caattttcta acaaatatct
    27661 aataacagcc caaaactgta aacctacaat tgctggtttt cttaaaccat ctgaattgcc
    27721 tacaggaata ccacatgatg actgacttac acctaagcca cattcattca taccttcacc
    27781 tcttccaaat tgcataatgg tagtacctat atgtgcatat tttccattta ccttagtttt
    27841 acataaaaca aaatcctcca tattgtgtga gaaatcgaaa ttacgtgcaa atagtacatg
    27901 cccattttca gttaattctg gtgctaatgc aacttgacta caacctggtt taagataagt
    27961 catagtataa tataaaattt gattacaatt cacttgaatt gcatcagcaa aaccttgcaa
    28021 ttcttcatta agacctggac aatatttatc aaacaactct atcatctctt tttcttcttt
    28081 tttgcttacc agattagatt tattaatctg accttctatt atctctggca tattagcaac
    28141 caattctcct agtctgtatc ctatctcata atttgtacca ctcaattcaa tatttcttac
    28201 ttctaaagtt tccatatgta cccctcctat atatcttata atcgatttta tcattcgaat
    28261 tgtaatcatt catgatattt tgtgctacaa gttaaaaaag gtcatataaa aacagataag
    28321 cctctttttc atgtttaaaa taataaaaat atatttaatt tcatacaaaa aagagtgtct
    28381 cataaactaa aaagttcatt ttgagacact cttcttattt taaagagtat aattaaaaat
    28441 agtctaagtt ctactcttcc ttcatttttt tcaataattc tggattttca aataaattaa
    28501 atcctgttaa agctaatatt tttataccat caattactct atcttttgca tatgaattta
    28561 tagaagcctc caaaaattct tttgtatgta gttcttctcc attttcagaa aacttcatca
    28621 aaggatgtat acatggcatt atttgactta aatttcccat atctgttgaa cctaacatta
    28681 cttgttcatc ttcatatatt ccaaattcct ccataatctt tgtaaatatg tctaccaaaa
    28741 ctttattttc atttaatcct tggcaagttg gttcaaagtg ataaaattca agttcacatc
    28801 cagtggctat tgcagcacct ctagcacaat ttttcacttt ttctactact tcatcaaagt
    28861 aattttgttc ttttgccctt atctctaatc ttacttttcc catatcaggt attacatttg
    28921 cagcagttcc tgcttctaat ataataccat gtattctcgt tccatctact aattgttgtc
    28981 ttagtgcatt tatattatta tatagtagta ctactgcatc taaagcattt attccgttat
    29041 atggcttagc tcctgcatga gatgcttttc cagtaaatat gaaatctaga cctcctattg
    29101 ctataatttc aggtgttaca caagtttttg aatttggatg tatcatcatt gctgcatcat
    29161 agccatcaaa tacaccatgt tctatcaagt aaggctttcc atctccagtt tcttcagcag
    29221 gtgttccaat tatacttact tctcccttgt aagtatccag tatacttgaa agagctattc
    29281 cagctccaac tcccatagat gcaattaaat gatgtccaca tgcatgtcca ttgcctggaa
    29341 gagcatcata ttctgctata aatgctattt taggaccatc tccatttttc ttggttgctt
    29401 taaaagctgt agccatacct gccaagtttt cctcaacatc aaaaccctct tctcttagaa
    29461 attctgatat ttttttagca gatttatatt cttgcaaagc aatttctggt tcatcatata
    29521 aagattgaca tagccctaga attttttctt tctttgaatc agccacttgt attattcttt
    29581 ctttcatacc aatcttacct cccactaaat ttatttagta atttttatat tttattataa
    29641 ctttaattta actcatgctt atcctctttt aagtatctat tttcccaaaa ctcagcattt
    29701 cttatatcta actctatagg gttaaaagtt ctatcactcc tttttattcc catcttcttc
    29761 tgtcttatat aatcataata tgcctttata gctggttttt ttagtgtaaa gatggcaatt
    29821 aaatttatat atgttaatac acctatacat atatcaccta atgcccagct tgtaagtggt
    29881 tctataaaac aacacattgc tgcacatgct ataagcataa atctacagac attaattatg
    29941 tacttttcat gtttttctgc tttaattcta gttactaagt aagatacatt agtctccatc
    30001 atatatgaga aggctaataa agttgtaaat gtaaataata ataaaactat tgctataaaa
    30061 atatcaccta attgtggatg taatgtattt attgcactct gtacatattg tggtcccggt
    30121 tctacacctg gtagtgctgt gcaaacacct gtaacattat acattcctgt tattacaatc
    30181 ataatacctg tagccataca tattaataat gtgtctacat aaacagaaaa tgcttgaact
    30241 aatccttgtt tggctggatg acttacttct gctgctgccg caccataagt cccagaacct
    30301 tctcctgctt catttgagta tatacctctt tttactcccc aagatattgc tgaacctagc
    30361 attcctccaa aaactgaacc aaatccaaat gcagatttaa acattgttac aatagcctct
    30421 ggaactaaac taatatttgt tataattatt actaatgtca taataaggta tgctccaccc
    30481 attataggta ctactttttc agcaaataaa ccaatccttt taattcctcc aaaaattatt
    30541 atcgctaatg caatagaaga tgctactgct attgtcattt ttgaaaatcc aaatgcttga
    30601 ttcattgcta gatataaagt gtttgattgt gtaccaggca taaaaaatgt ataacttatt
    30661 attgcaacta ttgaaaataa aactgaacac tttttccacc caagaccttt ttcaaaataa
    30721 tatggaggtc ctcctctata ctgcccatca tgctcatctt tatatatttg agctaaagta
    30781 gattctacaa atgcagttgc tgaacctaat gtagccatta tacacatcca tactaatgct
    30841 cctggacctc ccattgctat tgctacagct acaccagcta tatttccagt ccctattctt
    30901 ccagccatag atgtagcaaa accttgaaat gatgatatac caacatgaga actatctccc
    30961 tcaagcaaga gagtaaacat ttcttttaca tctcttattt gaaaaaattt taatttcaat
    31021 gaaaaatata atcctataca caaacacatt gcaactaata cgggtgacca aacaattcca
    31081 ttaatggtgt taactaaatc ggtcacttaa ctcaccacca ttcattttat atttatagac
    31141 aataatttaa acagctatat ttcaatattt tatataatct ttatattaat atatctaaaa
    31201 aatctacctt ctatttattt atattatctt taatctacaa tctacaaata tttatttcta
    31261 aaatcaagag tattaatcat atctaaacat aaatttataa aatttttaac gacgtgtgtt
    31321 tgaataataa tcaaacaata taaattggaa tacatataat gaaaagcctg aaa
//
LOCUS       DAYAOY010000046        31232 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYAOY010000046 DAYAOY010000000
VERSION     DAYAOY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.046"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            322..1158
                     /locus_tag="ACGI6B_002132"
     CDS             322..1158
                     /locus_tag="ACGI6B_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9751575.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1511..3088
                     /locus_tag="ACGI6B_002133"
     CDS             1511..3088
                     /locus_tag="ACGI6B_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9751576.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3081..4265
                     /locus_tag="ACGI6B_002134"
     CDS             3081..4265
                     /locus_tag="ACGI6B_002134"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9751577.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4283..4882
                     /locus_tag="ACGI6B_002135"
     CDS             4283..4882
                     /locus_tag="ACGI6B_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9751578.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4906..5376
                     /locus_tag="ACGI6B_002136"
     CDS             4906..5376
                     /locus_tag="ACGI6B_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9751579.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5735..6481
                     /locus_tag="ACGI6B_002137"
     CDS             5735..6481
                     /locus_tag="ACGI6B_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFG9751580.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6542..7216
                     /locus_tag="ACGI6B_002138"
     CDS             6542..7216
                     /locus_tag="ACGI6B_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9751581.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            7584..8930
                     /locus_tag="ACGI6B_002139"
     CDS             7584..8930
                     /locus_tag="ACGI6B_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFG9751582.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8957..10357
                     /locus_tag="ACGI6B_002140"
     CDS             8957..10357
                     /locus_tag="ACGI6B_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFG9751583.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10567..12003
                     /locus_tag="ACGI6B_002141"
     CDS             10567..12003
                     /locus_tag="ACGI6B_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFG9751584.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12347..13291
                     /locus_tag="ACGI6B_002142"
     CDS             12347..13291
                     /locus_tag="ACGI6B_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HFG9751585.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13460..14392
                     /locus_tag="ACGI6B_002143"
     CDS             13460..14392
                     /locus_tag="ACGI6B_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFG9751586.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14497..14964
                     /locus_tag="ACGI6B_002144"
     CDS             14497..14964
                     /locus_tag="ACGI6B_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HFG9751587.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15208..16080)
                     /locus_tag="ACGI6B_002145"
     CDS             complement(15208..16080)
                     /locus_tag="ACGI6B_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFG9751588.1"
                     /translation="MKGNSLMLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSIS
                     AFIFSTIITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQ
                     LTFTTLLAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVL
                     LSSLFLSLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLIT
                     GILWSIANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGI
                     ILIMSGVFMLSIIK"
     gene            16228..18192
                     /locus_tag="ACGI6B_002146"
     CDS             16228..18192
                     /locus_tag="ACGI6B_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751589.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18192..18653
                     /locus_tag="ACGI6B_002147"
     CDS             18192..18653
                     /locus_tag="ACGI6B_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9751590.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18656..18976
                     /locus_tag="ACGI6B_002148"
     CDS             18656..18976
                     /locus_tag="ACGI6B_002148"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9751591.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18992..20092
                     /locus_tag="ACGI6B_002149"
     CDS             18992..20092
                     /locus_tag="ACGI6B_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9751592.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20212..22812
                     /locus_tag="ACGI6B_002150"
     CDS             20212..22812
                     /locus_tag="ACGI6B_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFG9751593.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            23281..23616
                     /locus_tag="ACGI6B_002151"
     CDS             23281..23616
                     /locus_tag="ACGI6B_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9751594.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            23753..25030
                     /locus_tag="ACGI6B_002152"
     CDS             23753..25030
                     /locus_tag="ACGI6B_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HFG9751595.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            25151..26539
                     /locus_tag="ACGI6B_002153"
     CDS             25151..26539
                     /locus_tag="ACGI6B_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9751596.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            26985..27623
                     /locus_tag="ACGI6B_002154"
     CDS             26985..27623
                     /locus_tag="ACGI6B_002154"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HFG9751597.1"
                     /translation="MKSKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVALSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            27741..28610
                     /locus_tag="ACGI6B_002155"
     CDS             27741..28610
                     /locus_tag="ACGI6B_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9751598.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(28765..29910)
                     /locus_tag="ACGI6B_002156"
     CDS             complement(28765..29910)
                     /locus_tag="ACGI6B_002156"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9751599.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(30243..31019)
                     /locus_tag="ACGI6B_002157"
     CDS             complement(30243..31019)
                     /locus_tag="ACGI6B_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFG9751600.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT        12243 a         3280 c         5385 g        10324 t
ORIGIN      
        1 atctgaattt caataaatat atatataatt aagttgaaac acttgaaaat actggaaaaa
       61 attgaaaata gtttttgtaa tataaaatgt cgattgattt taaataatta aatatacata
      121 agaaataatt aaatatattt caatgaaaat atgataaaat atgacaaatt aattgatgac
      181 aataaaaaat attttgttat tgtagtatta tagggtaaat tgcaaaatat atacgaaagt
      241 aaaattttaa atattgacat taaattaaat atactatact atgtaagtgt gatgagagaa
      301 tttttttaga ggggatttat agtgaatata aaaaagatat ttaataacaa tgtagtagta
      361 agctccctag aagatggcac agaaataatt gtcactggtg ctggagtagg atttaagaaa
      421 aaagtaggcg atttgataga tgaaaatttg atttctaaga aatattttgt tcaagacgat
      481 caaagggaca aatacaatca aatacttaat aagacctcta ttgaatactt taaaatttca
      541 gaagaaatta tagaaaaggc taatgaagtt ttaaatactc aagtgaatga ttcaataata
      601 ttagccttaa ctagtcacat agaatttgct gtccaaagag aaaaacaagg tataaaatta
      661 ccaaacttaa tattgaatga gacaaaacaa ctttataggg aagaatttga atttggatta
      721 tgggcaattg atgaaattga aaaaaggata ggaataaaat tgcctgaaga tgaagcagga
      781 tatattgcta ttcacataat caatggtttg gaaaattctc aaaaagatga aggtattaat
      841 atactagagt tttcaaaaca agttattcag ataattgaag aagtacatgg atttaaattg
      901 gatgtaaatt cattaaatta tactagactt ataactcact taaagttttt tgttcaaagg
      961 attctccgaa aagaaactta caatgatagt gatattgaag atatgtataa gttagtgaat
     1021 aaaaattaca ataaacctaa agtgtgtact gaagaaattg cagcattggt attagacaga
     1081 tttgaatata agattagtaa agaagaagaa ttatatttaa tgattcacat aaataaaata
     1141 acaaacagtg gttgttagga ttgtaactga taaagcaggc aaaacctaag tcattgagct
     1201 taactattag attaagtttt ttgatttagg tttttttatt agatacaatc aaaatacaga
     1261 cagtgacttt taggattgta actggttaag caggcaaaac ctaaatcatt gaatttaact
     1321 attaggttaa gttttttgat ttaggttttt ttattagata caatcaaata cagacagtgg
     1381 ttgttaggat tgtaactggt tgagcaggca aaacctaaat cattaaactt aattattaaa
     1441 ttaagttttt tgatttaggt ttttctataa atttgattat gtttttagat aaaaatagga
     1501 ggggaaaatt atgaaaaaaa ataagttagg tttatggaac ttcttccaaa tgttaggaaa
     1561 aacgtttatg ttcccaatag cactacttag tgtttctggg atgatgctag gacttggggc
     1621 aggttttaca gaccctaaga tgattgaaat ggttccattt ttagggaatg aatatgttaa
     1681 cttagtttta aacttcatgt taacaatagg attatttgca tttaacaact tgggagcatt
     1741 atttgcaatg gcaattccac taggattatt aaaaaaagaa aaagagtttg gtgctttttc
     1801 aggattagta ggatttatag caatgcatat aggtacaaat ttttatttaa caagagctga
     1861 tttgttagta ccagcagaac agatgtcaac taatggtcaa gctatgatta tgggtataca
     1921 aacatataat acaagtgttt taggtggtat tgtagcagga ttaattgttt atgcaatata
     1981 tgataaagtt agtaatttaa agataccaga atcattagga ttctacagtg gaccaagatt
     2041 agttccaatt gttagtttaa tagttatgag tatagttgga ttattaatac ctattgtttg
     2101 gccaccattt ttcaatgcat tccaaagttt aggaagatgg atttcttcag caggaccatt
     2161 aggatatttt tcatatgcag tggcagaaag agttacaatt ccatttggac taaatcatct
     2221 tgtaacatct gtattccgtt ttacaccaat aggtggaact gctgttattg atggagaaac
     2281 gtactttggt acattgaaca tgtttatggc atatgtagaa aataatcaga ttatacctct
     2341 tgatttagct ggtaaaatgg aacaaggtaa attgatgatt caatatggat tagctggtgc
     2401 tgcattggct atctaccgta cagcaaaacc agaaaataga aaggcactta aaggactttt
     2461 aatatcagga gtgttgacag ttattatagg tggtataagt gaaccaatag agtttctatt
     2521 cttattcata tcaccagcat tatttgcttt ccatacattt atgaatggtc tagcaaacat
     2581 ggtacttcca tacttaggcg tgttgatggg attcacagga gatttaattg catttataag
     2641 ttttggtgtg ttaagaggaa cagctacagg atggccaata gcagtattag tagcagtttt
     2701 atattttgca atatactatt ttgtatttaa gtgggcaatt attaagttta atataaagac
     2761 cccaggacgt gaggataaag aaataaaagt atctgaagga aatggttcaa atttcaaaaa
     2821 tttgtctaca tataaaggac aacaaatgat atctgcttta ggtggtcaat ctaatatatt
     2881 atcactagat aattgtgtaa ctagattacg tttaaaatta caagatgtat cattaataaa
     2941 tgaggatgca attaaagagg ctggtggtat agcagttgtt aaattggatg agcatacaat
     3001 acaagtaatt ataggaaccc aagtgtattc tttaagaaag cagatggata aagctatgga
     3061 aggtcatttg gaggcatgta atgattaatt ttgataaaaa agtagaccga ttgggtacat
     3121 attgtaccca gtgggactat gtagaagata gatttggtaa gaaggatttg cttcctttta
     3181 caatttcaga tatggattta gagtttccat ttgaaataac agaggtgttg caggaaaggt
     3241 taaatcatag agttttggga tacagtagat ggaaacatga tgattttaga aattcagtta
     3301 cgaattggta tattaaaaga tttaattgta gcgttgatag taactggata tattatagtc
     3361 caagtgtaat gtattcaatt agcaaactta ttgagatgtt aagtaatgaa ggagatggaa
     3421 tccttataaa tactcctgca tacaatgctt tttatgatgt gataataaat aacaatcgta
     3481 atattataaa gtcacatctt gtaaatagga atggattcta ttcaattgat tttaatgatt
     3541 ttgagaaaaa gtgtaaaaaa tctaagatat ttattttatg taatcctcac aatccaactg
     3601 gtagagtatg gagtgagtta gaacttgtta agatgatagg aatttgtaag aataatgatg
     3661 tcaagataat atcagatgat atacacatgg atattgtata tgacaataga atgacaccta
     3721 tcttaaatgt tgcaaaggac tatttagatt ctgtattcat ttgtacatca gcgtctaaat
     3781 ctttcaatat accagcattg actgggtcat atgtgattat accaaatgaa ggtattagaa
     3841 ataaatttga aaatatcact agataccgtg attttgtaaa ttcgccagca attctagcaa
     3901 tattagccac aatggtctgt tatgatgact gcgaatattg gttagatgaa ctattaaaat
     3961 accttaaaga gaatcttaaa tatacgattg attatataaa agataattta gaaccattga
     4021 aattggtcat gccagaaggc tgttactttg cttggataga tttttccgaa cttaaaataa
     4081 gtggtgagaa attccaaaaa ttattgatgg atattggaga agttgctatt atgtcggggt
     4141 ctgtatatgg tgaagaaggt gagttttatt taaggttaaa tgtggcatgt tcaagagaaa
     4201 aacttgaaga tggtttaaat aggattaaaa aaactatgga ttatattgaa aaaagaattt
     4261 tatagtagga agggagatag ttatgggata tttaaaaaca atggttgatg ttatgactac
     4321 tgaaatgaat gccataagat gtttaataga tgaagcagga atagaatatg aaagtatagt
     4381 tagtgaaatt gcaaattgtg aaggcaaagt aatttttatg ggtgtaggaa aatctgcaca
     4441 tataggtaag aaattggcag ctacatttgc tagtactggt acacctagct tttttgtaca
     4501 tgcaacagag gcagtgcatg gagatttagg aatgattgaa agtaaggata ttacaatttt
     4561 aatatcaaat agtggaaatt ctatggaagt ggtaaactgt ataaaatata ttaaagcaat
     4621 aggttctaag acaattgcat ttacttcaaa cagaaattca gtactggcaa aagagtgtga
     4681 ttatgcttta atatatcctg caaaggatga agcagaccat ttaaatctag caccaacaac
     4741 atcatctaca attactttgg ttttaggaga ttctattgca tgtgcattat ctaagagtag
     4801 taattttggt tcttcagatt tttataagta ccatccagga ggaagtttag gtgagaagtt
     4861 aaaaacagct aacaatggat aaaagcaaaa aggagagtag tttttatgtt caaaatgttt
     4921 aaaaaaaaat caataaaatc acccatatca ggaaaagtaa tagagttatc taaaatacaa
     4981 gatggtgtat tttctcaaaa aattatggga gagggtgtag ctatagattc tacaggagac
     5041 attgtatatg ctccaatcaa tggaaagata gtagtagtag cagagactaa acatgcattt
     5101 gtaatagaat tagaaaatgg aatggaactt cttattcatg ttggtttaga tactgtaaat
     5161 ttaaaaggcg aaggttttga ggcattggtg agtatgggag aacaagtgaa ggaaggaaca
     5221 ccaatgttga aaattaaccg tcctttgatt gaatcaaagg gtatatcact tattactcca
     5281 gttacaataa ctaatcattc agagtacaat atgaatacat gtaatgtagg caatagtgtt
     5341 gaaggtggga aagatactgt aattgagttt aaataaagaa ctaaaataca aggttaatat
     5401 ttataaatac ctataaactt atgttttata agattgattg tatagatttg ttgtataaaa
     5461 aagaactctt taattataaa ttaaggagtt cttttttatt ttgtaaatat tgtttagatt
     5521 tatatagaaa aacacttagt aataaataga ataaataagt atttatatag ctaaaaatct
     5581 aaatagtttt gtttaaatat atttttatat tttgtaattt caaattaata aataattaga
     5641 tttgataaac aaaaaataaa aatgttgatt atattaataa actttaatat aatatattta
     5701 tgaaaaatgt aaactgtgaa atggaggtag taacatgaaa attgaaaatc tttctattga
     5761 tgatattaaa cgaggctata tatttaatga tgatttaaat tgttatagtt gcattaaatg
     5821 tacaaaaaca tatgaggtag gagaaatata taaaataggt aatagattct ttgaagcttc
     5881 aaaagctatt gaacttcata tggaattaga acatagtgat tatttagagc aattgcttaa
     5941 tagtgattca aaatacaaca cattgactga aaatcaaaaa caattatttc aattatttat
     6001 tagtgattta ccagataagg agatagcaaa acaggttgga gtatccacat ctactatacg
     6061 ccatcaaaaa tttattttca gagaaaaagc aaaacaggct aagctctatt tagcactata
     6121 tgaatctgta tttgaagata aatctaataa aagtagtact ataataccaa tccatgaaca
     6181 tgctcgaatg gtagatgaaa gatatgtagt aactgaagaa gagagaaaac acattttgga
     6241 tacatcattt gaaagtatga atcctttaaa attaaaagta ttttcttcaa aagaaaagaa
     6301 aaaggtcgta attttatcta agattttgga acaatttgaa cgtgggaaga aatattcaga
     6361 aaaggaagtt aatcagattt tagaatctat atatgatgat tttgcaacaa ttagaagata
     6421 tctaattgag tatggtttta tgagtagaaa taaagaatgt acaaaatatt ggttaacata
     6481 attaaaataa taaattttac taatgtgtaa tgttattttt atctaggggg aggagtcaaa
     6541 tatgaatgat gaattaaaat ctattttata caataaaggt gtggacattg ttcgctttgt
     6601 tgatatatct gaatttccaa taaatcaaac gcaaggtttt tctaaagcaa ttttattttg
     6661 tatagggtta tcaaaaaaat ttattacaga tatatacaat aacttaccta cagatagtga
     6721 tgagttttta gaaaaagagg agaaagcaga agaattagct gactggattt cagaatatat
     6781 tcaaaataaa ggatatagag cgtattctca atcggagaaa aacaatctag aacatggata
     6841 ttttgaaaaa gcctatatta atccagaaat gcaatcaggg attagtcctt taccacataa
     6901 gacaatagca aatattagtg gcattggatt tatgggaaaa aataatttgt ttgtaacaga
     6961 ggaatacgga tgtgctttta gcatgtgtac agtacttaca gatgctccaa tttctgttga
     7021 aagatatcct ttgattgatt caaaatgtat ggattgtaat gtttgtgtag aaaactgccc
     7081 tgcaaaagct atacatggaa atgagtggac tttgccagga aaaagagagt ctattattga
     7141 tgtttcaaag tgtttttgtg tattgaagtg tatgatgagt tgcccatggt cattaagata
     7201 tgcaaatcaa aaataatttt actagagaag gtattaaatt gaatattata aaatttttaa
     7261 aatgtaagat tatataagat ttttcaaaat aaaaatttgt atagaacact cagaaatgag
     7321 tgttttttta ttggaattaa attatattga tacaaagttt aactatttta atcattatat
     7381 aaaatttgta ttaaaaaatt aagaataact tggtaatagt gaaaatataa tgagattata
     7441 attgatttat aatataacta taatgatgtt gtaaaatgaa aattgtcatt tcaaaattcc
     7501 caaaaagagt aaaagtcact tcggttatag tcaaaaaatt ttttcagtga aaagaattta
     7561 gatttaaaag ggaggaatta atcatggaaa ttgaaactaa aactccggat tggattaatg
     7621 tactagaatt aaacagtaaa atagatatta ctgggaaata tgatgtaagt aatattatac
     7681 aagatattgt ctctgataag tcactagaag gttccattat atattttcca aaaggtaatt
     7741 atttatttga gaaaggtatt aaaattagtc aacaaataac acttcaagga gattcttatt
     7801 atggaggaga taatcaagta tcaaatttag ataaaaaaca atcaatcata ggaacaacca
     7861 attttataac tcgaggagtt tctaatatga gtattattgc attgacagga acaagtcaat
     7921 gtatcaaaaa tattaatttt tattatgata gtcatgatat agagaaaata ccgcctaaaa
     7981 atgtatctgc tattacagaa tatggagaaa cacaaggact atctcatttt gaacacctgt
     8041 ttatttctgg attttcaggg atagggattg aaattccata ctattcaact ggcaatgata
     8101 taattgttag ttcatgtggt cttggtatga ggcttggaga gaagtcaatg ctttctagta
     8161 gcaagattta cgaatgtaaa aatggaatgg agattactac tggtgttagc ttaaataatg
     8221 tgagaataga agaaataaaa gaaataggta ttaataataa aggatttggt tttaacctga
     8281 ttatgaatct tatggtcgac caatgtggat attgtggatt tatgtttgaa aaaatgtgct
     8341 atagccagat aactgccaga attactagat gcggtcagta ttataaaagt gttgattata
     8401 atgcttatga gaatatggaa gatagaaaag aaaaagctta ttccattttt tatggagata
     8461 ttttagaaag ttgcaatatt gtattgatga atagaaatat tgatacctta gaaagtgatt
     8521 cacttaatga acataagata tatgtaatca aggcaaatga aacaaacaat gtaatgctaa
     8581 catgttatgc tgaagcagat aactttatac aatgtgcaaa gggcaacttg ctgttagaaa
     8641 atggtgaaaa tacttataaa ttttataatg gtgagatttg ttctgtagga ggtgttcaaa
     8701 tttctgatat agatgaaaaa gatttaatca aaataataga gaacactttg tatatagatg
     8761 ataaaggtaa gtatttaatg gttcctagat taaaaaagaa tactgtaata tattcagtac
     8821 tagattctac agaagaaata aataaattac atggtggaat atgggaacag attgatgtaa
     8881 aagtattagc tggggaatcc atgtactatt ataaaaagat aaaagattaa attatcaaac
     8941 taggaggtag tgattaatgg aagagtctat aataattaaa gatataagtg gctggattaa
     9001 tgtacttgat tatggaattt gtaatgatga aacaaaagat gtaagtttta agttgcaaga
     9061 gattatggat gctgtaccag aagattctat tatatatttt ccaaaaggaa aatacttatt
     9121 tgaaagtggt gttaagatta agaaacgatt aaccttatgt ggagattctt atattttagc
     9181 gaatgttcct aaaaatactg ctggagtaac acagtttact tttaaaggaa agaaaaatcc
     9241 taaaagtgaa aatagagcaa tcataacagc aaaaagtgta agacactgta ttaagaacat
     9301 ttgcttttat tcagatagtt gtgatagaga tatgacatct aatcatgaac ctccaacaga
     9361 tggaaaacct agatttcatc ataatgtaat tataaattat agaaatatat ctgctgttat
     9421 atgtgaagat aaacagggag aaccaggtca ttatgaaaat ctatctttta gtggtttttc
     9481 tggaattgca cttgatatgc ctaataattc tacagcaaat gatattacaa tttttacatc
     9541 cggacttgga attagtacag gggaaaattc aacaattgct aacagtaagg tttggggctg
     9601 tgttgatgga atgataattt ctactggaac ttttataaat ggtatgaggg tagaagaaat
     9661 aaaaaaagtt ggaattaaaa atattggtag aggtttaaat tttattacaa atatcacaat
     9721 tgaccaatgt ggatattgtg gagtttggtt tgattctata tccaatgttc agatatcagg
     9781 aaatatcaca aggtgtgggc agtactatta tgatgtaagc tatgataatt atttaatgtt
     9841 ggataataga gttaaagaag catattcttt attctatggt aatttattgg agtcaagtaa
     9901 tatagtttta tcaaatacca atgaagataa ttgggaagat ttcaaagaag ataagcataa
     9961 ggtgtatctt atagaagcac ttaaaaccaa gaatgtacta ttaacatgca atatagatgc
    10021 tactgactgg attgttaagt cagaaaaagg aaatttaacg tacaataata tgcgacaaac
    10081 tgttatattt tacgatggaa aactaagtag tatcaatgga attggtatat ctgatgaaga
    10141 ttatggggat aaagtcaaga taaaaaaagg aaatatatat ataaatagta atgataagga
    10201 tattaagatg cataaaccag aggtaggtga cattatgtca acaattatta aagataaaag
    10261 tattctttct gctcattatg ggggaacatg ggagaaaatt ggtgaaaaaa tagagtttgg
    10321 aataactgta cactattata aattgataga gaaataagta aatacgtagt atttgaatag
    10381 atgtatttta ttggagagtt aggactatag ttttaatata aatggtttgg taatagcatg
    10441 gattgaataa tataaaaata atataaaaaa ttaatttgat tatgtaaatt tctatgaagt
    10501 agtaaaactg tgactttagt cataggaggt tcaaactaag taaaagggaa aggaatatat
    10561 aatattatgg aaggaagaac tgggattaaa gatggaagat ggattaatgt agtagaaaga
    10621 ggtattaaaa atgatggttc gtacaacata ggtcctgctt tgcaggatat tattgatgaa
    10681 atagaaacag atttttgtgt cttatatttt ccaaaaggta aatacctatt tacaagtggt
    10741 gttaaaatta ataaagaaat tgtattgcaa ggtgattcta attcatctac aggtactaca
    10801 caatttataa ctcgtggagt agaaaatatg agtattatta cattgacagg gaagaaacaa
    10861 tgtattaaaa atataaattt ctattctgat agttgtgaga tacaggtaaa taaagaacca
    10921 ccgacaaaag gtaatcctaa atatcattat gagatgatta ttaataagaa tgaaaagaat
    10981 gttgagatga ataatgtatc agcaatcctg tatgataaca gtaagaatat gattaaaggt
    11041 ctaggtcatt atgaaaatat ttatataagt ggtttttcgg gtacaggaat taggatacca
    11101 tattactcaa ttgtaaatga tattacagtt tcatcttgtg ggctgggtat tgatacagga
    11161 atagacacaa ttatttctaa tagtagaata tctaagtgtc aaaatggaat gaggattaga
    11221 actggaacta gtataaataa tgtaaggatt gaaaaaatac agaaagttgg tcttataagt
    11281 agtcaagtta aggggtatga ggggtatgga agttataaaa ttaataatat tacgattgac
    11341 caatgtgggt attgtggctt ttcatttgat tcaatgaaag atagttattt ttctggtatt
    11401 ataagaaggt gtggtcagta ttattataat acagattatg acacatattt aagtattaaa
    11461 gatagagtag aagaagcata ttctttattt tatggaaatt attttacaga atgtaatatt
    11521 gatttagtta gtgataataa ggataactgg gatgatggct tagaatataa acataaagtt
    11581 tatgtattta aaacccttca aataccaaaa ttaacattga gatgcaatat agatgcctat
    11641 gattatatcg ttaaatcggt acatggagga ggtaatttaa tgttaaaaaa taactttaac
    11701 aattatatat ttattgattc atctaatcca gaagttatta atggaattaa ttttaattat
    11761 caaaagaatg gaaacttgtt aaggttaaat aatggtaaat taagttataa aaatggaaag
    11821 gaagaaacag ctatattcag acctaattat ggtcttattg tagcatcaac agattcagaa
    11881 tgtgaacaaa tagaggctac ctatggagaa aaatgggaga agcttggttc tgaagtaatt
    11941 ggagaagaaa cggtatatta ttataaacat catgaaccag ataaaacaaa ccaaatacaa
    12001 taaaatttgt attatataat gatataaagt tttaatatat agtgtttacc tttaatgtaa
    12061 tattggtata tttgtatagt attaaatttg ttaaaaagta aaattgtagg gtgcttgaaa
    12121 atagcacctt ttttagtttc attataaaat tagttttgaa agtttaggaa taatgtatat
    12181 ttatttttaa agaattataa ccttattaag gatattttaa atgctagaaa tgaatcagta
    12241 aatcatactg gaattatctt aaatgttttg attaaattaa gtatttagag ctataattat
    12301 attaaaaata tattagaaca taattatatt actatggagg ttaaatatga tagtaagtgt
    12361 gagtagaaga actgatatac ctgctttcta tagtaagtgg ttttttaaca gacttgaaga
    12421 aggttttgtt tatgtaatta atccaatgaa tccaaaacag gttagtaaga tagagttaaa
    12481 tccacatact gttgattgtt ttgtattttg gacaaaggat gtaactccaa tgttatatga
    12541 tttaaataaa ttaagagact ataaatatta ttttcactat actataacat catatggaaa
    12601 agaagtagag acatgtattt tagataaaag aaaggtaata gattcattta aggaattgtc
    12661 tagaaaaata ggcaaagaaa gagtaatttt aagatacgac ccgattttct taagtgaaaa
    12721 gtacacagta gattatcata tcaaagcatt tgcgagttta tgtaatcagc ttgatggatt
    12781 tacagaaaaa tgtataataa gttttatcga tttgtataag aaaacaaaat ttaatactaa
    12841 agcattacat ataaagccaa tagaaattac agaaatagaa acattatcaa aagagatttc
    12901 taagattgct cataagcatg ggataatact tgaaatgtgt tctgaagaat ataatttaag
    12961 taaatttggg attggaaaaa gtaagtgtat tgatgataga ttaatatcaa aaattatagg
    13021 atgtgaagta gaggtaaaaa aagatagtac acaaagagat atttgtggat gtgttaaaag
    13081 tgtcgatata ggacaataca atacttgcag acattattgt ctgtattgtt atgctaactt
    13141 taattatgca aaagtagaag aaaactgtag gctttataag gaagataata aattactcat
    13201 agatacttta agagatgatg caaaaataag cattagagat atgaagacta ttaaagtaga
    13261 taagaataaa caaattagta tatttgatta aaataaatat actaattacc ttaatatatt
    13321 aagaatatag atacttttga taacaaaatt aaacttaata gatattttct atataagaaa
    13381 gatggtcata atatgtcaat ttgaccatct ttttgatata attaaaacac aaataattta
    13441 gtaacaaagg aggcagaact tgaatttatt aaaaaaatat cctataccta ttgcaagttt
    13501 gattttatca atttttactt taggtaactt attacaatct tatagtgaat cattacgtaa
    13561 tgtaattgga tttataggat ttattttgta tgcaatttat atagtcaaaa taatattact
    13621 tagaaagggt gttaaagagc aattagagaa ccctttaata tcaagtacat ttccaactat
    13681 aacaatggca acaatgatat ttgctacata tataaaacca ttatctttag aattaagtat
    13741 tatgatttgg agcataggaa ttgtgggtca tcttgtgtta atagtcttat ttagtaagaa
    13801 gttcttagtt aaattctcaa taaaaacggt atttccatct tggtatatag tatatgttgg
    13861 gatagttgca gctagtgtta cggcaccagc atttaatcaa cttttgattg gcaaaattgc
    13921 cttttggata ggatttgtat cttatatagt tcttatacca gtaattttaa agagagtatg
    13981 gataatcaag gaaatgcctg aaccatcctt accaagtata gctatattag ctgcaccagg
    14041 gtcactatta ttgtctggat atattaattc tttttcagat aaaaataatg tagtattata
    14101 cttgttattc atattatcaa ttgtatttta tattatagtt ttgggatatt taccgaaatt
    14161 attaaggttg cctttttatc cgagttttgc tgcatttaca ttccctatgg caataagtgc
    14221 aatgggtatg aaactaatga atggttattt aactaaatca ggtcatttta taagttggat
    14281 ttcatatgct tctaagttag aagagataat tgctacagtg attattttat atgttttagt
    14341 tttgtttatt atatatctta gtaaatctga aactacatct aaagctaaat aagaattatt
    14401 aaatatgtgt atttaatagg aatattgata taaatatatg aagataagat aaaccataga
    14461 aacttaattt agcttaattt tattggagga aaacaaatga aaacaaaaga agaatattta
    14521 aaagaagtta gaaacatatc agaagaatac tttagaaaag gagagttttt ttgctctgaa
    14581 gcagttctcc agacaataaa tgatgcttta ggccaacctt taagtccaga gataactaag
    14641 ttagcatcag gatttccgat aggacttgga aaggcacaat gtctttgtgg agcaatatct
    14701 gggggagaga tggctttagg tatagtatat ggaagagttc atggtgaagc tatgaatcca
    14761 aagatgtttg agcatgctaa aggtttgcat gattatatta aaaaagagta tggtgcaaca
    14821 tgttgtagag ctattacaaa gaaatgggat ggtgataact ttatgtctcc cgaaagaaaa
    14881 gagcactgta ttaaaataac aggtcaagtt acagagtggg ttgcagataa gcttatagaa
    14941 gatgaaaaac ttaatatcaa atagtgtaat attagttggt gaatttataa agttaaattt
    15001 acttatagta taagatactt gatattctcg tattgataga aaaatttata gcatatgtat
    15061 ttaatgaaat atacttatat ttagagtgtg gtttaatttc ataaatacat atgctattat
    15121 tttaagtata taagcaaatt tatttaaata aatagaaaca gttaatttat atgtacaaaa
    15181 ctatatatga ttagtgtaaa taaataccta ttttataata ctcaacataa agacaccaga
    15241 catgattagt attataccaa taaaaatagc aagccattct tttttgttct tcttttcttt
    15301 aaaaaatata atccctccta aagtggctac tatcacacaa ctctgagaga tagaaaaact
    15361 tattgtaact cccaatgtat tagtcgttat gaacatacct aaatttgcta ttgaccacaa
    15421 tataccagta attaaattga ataaaacatt ttccctatat acaatctctt tagaaaaata
    15481 cttgacaata atagatgaag ttatcaacat accaacagat tgaggtaata taattctata
    15541 tccttcaatt aaaaatattt gattagttac aacatataat gataaaaata gagatgataa
    15601 taatacaact ccaacacatt ttaacatatt tttatctgta gattttttag tttgataatt
    15661 tgtgagaagt ataccaagta ttatacataa tattgataaa gaaccaatca gtaacatttt
    15721 agaactggtc cattcattaa acaagattac agctaataat gtcgtaaaag ttaattgtga
    15781 accatttgaa attggcatta ctttagatac actagcaatt tgaattgctt taaactgcat
    15841 taattgtccc aaactccaga atatgccaga aatcatactt atacaaaatg aaaaaaatgt
    15901 aatttcagga tgtataacca gagttattat agtggaaaat ataaacgcac ttatggatgt
    15961 acctaaaagc tgttcatgag gagaactttt ctttagattt gcaactatgg gcatcaatcc
    16021 ccatcccatt acaggaagta aagcaataat tattgtcagc attaaactat ttcctttcat
    16081 attaaattta gacagctttt aatttatcat ctaaaattaa agtgttcaat acataaaaat
    16141 taccgtattt aatcaggaaa tattagtagt tttttagaaa aacattttcc attaaaataa
    16201 agacaaaggg aaggagtaaa attgaaaatg ttaactgata tgattcaaaa tcgtgaaatt
    16261 aaaataataa agtttttatt aaaagaaggt catacaactg taaaagcaat agcagatgat
    16321 ttagatgttt cagaaaaaac agtttcaaaa tctcttaaag aagtagaagc agtgcttaaa
    16381 aagataggtc tttctttaat aagaaaacct aagataggtg tttatattga tggagattta
    16441 aaaaaaatac ttccttacct agataataaa ggaagtcaaa ttcctacaac cagagaggag
    16501 agagttatct atatatattc agtactttta aagagtaatg attatattac tatacaacaa
    16561 ctttctgatg aactatatat aagtcgtgga actattgaaa gggatttagc agaagttgaa
    16621 aaactgctaa aaaacgaagg gatttccata tataagaaac ctagtaaggg aataaagtta
    16681 aatataagtg aaagagataa aagacaactt actgctaagt ttatacataa attttggagt
    16741 agaaactggt atattaagca agagcaagaa agcttttatc aagcatttga aaagatacaa
    16801 gcagatgtaa atggtatttt ttcggaagat gatttatcaa taattattga tattcttagg
    16861 aatttcagca gagaaagaaa atttgagttc tctgattatg aatttcagtc attagttatt
    16921 catttggcaa tagctgtaga gagaattaga aaaggcgaat atatagaaga aagtttgtat
    16981 actggtaata tagaaaataa tcaaatgtca aataccaatt atttagcaaa acaattagaa
    17041 gaaaaatttt caatattatt accaaaatct gaaattggat atataaattt acatttagta
    17101 gcgtctgacc aacatagaga tactaatgaa ctaaaaaata cttcaactaa tccttgtgag
    17161 acaagtacaa ataatatatc aaatcttcgg gaaattataa aaaatacatt gtatgagaca
    17221 gcttatgaca aggatttaat tgatggatta acttcacatg tacattcatc aataaataga
    17281 ctgaaatatg gacttagtat taaaaatcca tatgtaaata caatcaaaca aaacttttct
    17341 ttatcatttg agcaagcttt agatttaaag aaagtaattg aatttatata tgatattagg
    17401 ataaatgatg atgaatgtgc ttatatagca ttacattttg aagcttttag agaaagaatg
    17461 aaagaacttc cacaaaatat tagagtaatt ctagtatgca gtacaggatt aggttcatct
    17521 aggttattgt ctgcaagaat aaaaaaatac tttccaatga taaagattga aaaagtactt
    17581 tccgtacaag aattagtatc cagtaaaatt gatgcagatt tagtaataag tactatttat
    17641 ttagagttag aaacaattcc aaccatagta gttagtccta ttttaaatga aagtgatata
    17701 agaaccttgg aaagaaatat acaaaatgtt tcaagttata cttctaagaa gtataaatct
    17761 ttcagaaact taatctttga agaaaatata ttgttgaata ttgaagcttc aaactatgaa
    17821 gatgcaatta agtatataac agataaccta ataaaaaaag gttttgcaaa agaaggtata
    17881 gctaaaagtg caattgagag agaaaattta tcttacactt ctttccaatc aatagctaca
    17941 cctcatgcta atcctagtta tattaaaaaa tctaatatat ctgttgcaac gttaaaaaat
    18001 cctattacat ggggaaatga aaaggtggaa gttattttct ttatttcatt agaaaatgac
    18061 aaaaacctag agcttgatga aatatatgaa gttttttatg acttcattga caataaaaaa
    18121 aatataaatg acatattaag tgcaacttcc acaaaggaag tgtacgattt gctagtggag
    18181 gagagtatat agtgaaaata tcagatgtat tgaaaaaaga gcaaattgtt tttaatcttg
    18241 atacaaatac aaaggaagaa acaataaaaa aattaagtag tatatttgtt gaaaatggga
    18301 ttgtggacaa tgaagaagaa tatgttaagt ctgttttgga aagagaagaa cattcaacta
    18361 caggtattgg taatggtatt gctattcctc atgggaaaag taatgctgtc aaaaaatctt
    18421 ctataatatt tgcaaagact aagcataaga ttgaatggga tgctttagat gaaaaaccag
    18481 tagatagtat tttcttattg gctataagca atattgataa ggatagcaat catcttgtac
    18541 tattatctaa attagcaact aaacttatgg atgatgacaa tgtagatgca ctaaaattag
    18601 ccaattcaga acaagaaata atcaatatat ttagtgaaga aggagagatg tagaaatgaa
    18661 aagaaaaatt atagcagtta cagcatgtgc aacaggagtt gcacatacat atatggcagc
    18721 tcaagcgtta aagaaagctt caaaaaagat gggtcacctt ataaaagttg aaacacaagg
    18781 agctacagga atcgataatg agttaactac aaaggatgca caaattgcag aagtagtaat
    18841 atttgcagta gatactaaag tcagaaatgc tgaacgtttt gagggaaaag aaatactaga
    18901 agtaccagtt aatgcaccta taaaggatgc agagaaagta ataaatgatg cactaaagtt
    18961 aattgacact aaataaatgg agggaaatgt aatgttgaaa aagatagtct ctgagttaaa
    19021 aaagcatgtc ttaactggaa tctcatatat gattccatta gtaattgcag gtgctatgat
    19081 aatggcgata tctcgtgtag gtggttcttt ctataatata ccagatatat gggatgctaa
    19141 atatgcagaa agtgctagta gtattgttag attattacat gatttagatg ggtttggagg
    19201 tactgcatta ggattaatgt ttcctgtaat tgctgcattt ataggatttt ctatagctga
    19261 taaattagct attgtaccag gtcttgttgg aggtatgatt gctaaagata taggtgcagg
    19321 atttttagga gctttggcag ttggtcttat agcaggatat acatgtttgt ttattaaaaa
    19381 tcatgttaaa ttaccaaaat cagcagcttc aatagtacca atatttatag taccagtatt
    19441 tgggacatta atcactgttg tattaatcaa ttatgtgata ggtatacctt ttgccacatt
    19501 aaatactgga ttagaaaatt ggttaaatgg attatctggt acaaatcaga tactaatggc
    19561 agctattata ggggctatga tggcagttga cttaggtgga ccagttaata aagcagcttt
    19621 aacaacatca cttgcattat taacaagtgg aatttatgcg cctaacactg ctgcaatggt
    19681 aggaatagtt ataccaccac taggattggg acttgctact atactagcaa aacaaaaata
    19741 taataaacag ttacgtgaag ctggaaaatc atctcttata atgggtttaa ttggagtaag
    19801 tgaaggagca attccttttg cggtagaatc tccattaaaa gtaatacctt catgtgtaat
    19861 tggaacagca attgcttcag ctatggcagt tggtttaggt tcagtaaatg caactccaat
    19921 tagtggattt tatggatggt ttacagtaga gaattggcca atgtatgtgt tatcaatagc
    19981 agttggaaca gtaattgtag ctggattagt tatagtattg cgtggaaatc cagtgattga
    20041 ggatgatgaa tttgaagatg atgattttga tgaagcagag tgggaatctt aaaatttaat
    20101 aactatttaa gacgtgttat aagctatatt caatatgaat cttatactaa ataaagtgtt
    20161 aaacattcag tttagactta aatataaaag ataaagaaca gaggagaata aatgataaaa
    20221 gcacatattg ttaatcatac acactgggat agagaatggt attttacttc tggagatgca
    20281 ttagtactta gtgaacaact atttacagat gtaattgatg aattagaacg taatccagat
    20341 gtaagttttg tacttgatgg acaactttct attttggatg attatgtaca attacacaaa
    20401 gaaaaaatag aaatcattaa aaagttaata aaagaagata gactacatat tggaccatgg
    20461 tttactcaaa ctgatgcatt ctttgctaga ggagagtcta ttttaagaaa cttaatgatt
    20521 ggaatttttg aaagtaaaaa gtatggtaag tatatgaaaa ttgggtattt gccagataca
    20581 tttggattta atgcacagat gcctatacta ttaaagcatg taggtcttga taatatattc
    20641 ttttggagag gtatacatct tggtaaacag gttcaatcac catattttaa gtggaaaagc
    20701 ttaaatggag aaagttatat atatgcagta aatatgccac atggatatgg aactggtatg
    20761 cttttagagc cttcattaaa gtatgttaat ggacgcttag acccagctat tgattttatt
    20821 aaatcatata ctgatgtaga tgaggtgtta ataccttctg ggaatgacca gttaaatata
    20881 ataggtgatt tcaaaaataa aattaaagcg attaatgata tcggaaagta ttcatatata
    20941 acgagttcat atcaagagtt tttaaaatat ataaagtcca tagaatcact agaaagctat
    21001 agaggagaat ttagagaacc tgtattagca agaatacata aatcaattgg ttctagtaga
    21061 atggatataa aattagcatg tgataaacta gaaaacaagc tgattaaaag aatagagcca
    21121 ttattagtaa ttgctaaaaa gagtaaaatt gaaataagca atcagttact tataaataca
    21181 tggaaaaaac ttttggaagg acaagcacac gatagtttag ctggatgtgt aactgatact
    21241 gtaacagatg atattcttca ccgtataaga gaagcaaatg aaatatgtga cagtattgaa
    21301 aatactattg taaaaaagat ttcagaaggg cttaaattat ctaaaaatga tattttagta
    21361 tttaatacag aagctaagcg ttttaatgga tataaagaga tacaagttgt aagtgatagt
    21421 aaaaatattt attttaaaga taatttagat gctactataa ttgaagaaac ttatgtaaaa
    21481 ccgagagaaa atgtattaga agaaacacct gctggtaata tatttattga agaaccagga
    21541 tattatatat taaaagtaag aataaatgta cagcttcctg cacttggtta taaagttgtt
    21601 agttttgaat caagtgataa agaaatggtg tcacttaata aatctaatga tgcttttata
    21661 tctaatgata attataagat aatatatgaa gatggaagct taaatttaca acttaaagat
    21721 ggaacttaca tatcaaactt tttaacatta aaagatagtg gtaatgcagg tgatacctat
    21781 gacttttctc cactaaaaaa tgatgaggat ataaaacttt cgtttgataa agtgtataca
    21841 gaaaaagctt taggatatga aaaaatggtg gttaaaggta ctactttact accacttacg
    21901 ttagaagata gaaaaaacaa atatcttaat gggaaattaa atgtaaaagt aagtattgtc
    21961 ttatccaaag aaaatccatt gatggatgta aaactttgtg ttgataatac gatatataat
    22021 catagactac gtgttcatat aaaaactgat ataaaagata ataaaaacat agcatcattg
    22081 ccatttggat atataacaag agaaaatgga gttttagaca actgggaaga tatttacagt
    22141 gaaatgccta tagatttaga acctttggaa agtaatatta ctctaacaaa ccaaaataga
    22201 agctgtacta tatttactaa aggaataaaa gagtatcaac atattgaaga tgaaattgca
    22261 ttgaccttac ttgctactac agatgaacta ggaaagcctg atttattata tagaccagga
    22321 cgtgcttctg gtgatacaac taaaaaaggt cacataagaa ttaaaacaga taaagctcaa
    22381 atgcttaaga aattagaatt ttcatttgca atatatatgg attctaaatc atttgatgaa
    22441 ttggaaattg cgaatttaac ccacaattat ctaagggaat gtgtaaatta tcaattacag
    22501 gattacaatt tctttttata tcgtatagat aataagattc aaaaatcaat tgtcaataaa
    22561 actgtaagta gagaattaga aataatatct ttaccagaaa actatcttat atcagcatgt
    22621 tatccttctt attacagtaa agataaattt ataataagag tggaaaatcc aagtagtaat
    22681 aaaatgatat tggatgagca aatatttaaa aatagaaatg ggaaaattgt aaatgcaata
    22741 gaagatgtag aagaggaaca ggtatatgaa atagcacctt ttgacgttat ttcaatatta
    22801 ttggatttat aagtgtaaaa gtaagtttat tgtttaaagt ctgacagata ttaatctgcc
    22861 agacttttta atttgtataa atttgggtaa atattaatga atggttttag tatttaagta
    22921 tgaatttagt atttaatata gaatttattg aagtagaata attagtttaa caggataagc
    22981 agtttaaata aagaattatt atattagagt gattttatat ataatttaag ataataaaat
    23041 tttaataaaa tgagttaaag attgtatatt aatagagttg atgtagttac aaaaaagtta
    23101 ccaattgtta tgtatttaca aaactgatat attaattaca taaaatctaa aatagtttgt
    23161 aataggagta tatataataa aaattttaat taaataaagc taggtatatt tataatatta
    23221 ataaatatat tgctataaaa gtttgtttaa taaattaaat aaagaaaaga ggagaataaa
    23281 atgtacaaca tagatgatat gatatataat tgtccagtag aagctttatc aaatatttta
    23341 ggtaaaaaat gggtggctat aattatatgg aaaatacaag aagataaaaa acgatttgga
    23401 gagctacaaa gagctatacc agactgtagt aagaaaatgt tagttcaaca actagatttt
    23461 ttaattgaac aggggattat agtcaaccaa aaaaagttgg ttaataatgt tgtagagtca
    23521 acatattttt taagtgaatc aggattacga ttgcttcctg tcatggaaaa aatgattatg
    23581 tggagcaata aaaatttaaa ttgtgataaa tactaaaatt tactctgtaa gtaatgaaaa
    23641 tttaattaag ctatgagtag aaaaatgtat taaaattaca taatttttta gttagatttt
    23701 aatttagaaa tgggattgta atattggaaa taaaataagg aggaatttta atttgcaaaa
    23761 tgtaaattta tttttagtat ttatagaagg tatagtatca ttcttttcac catgtatact
    23821 gccaattttg cctatttatt tgagtatatt gtcaaatagt agtgtagaaa atttaaaaga
    23881 aggaaagacg agttttatag gaagttcttt atttaaaaat actatatttt ttgctcttgg
    23941 aatctcaaca acattcttta tattgggttc atcagtaaaa gtattgagta tgttttttaa
    24001 tgaaaataaa gatttaataa tgtttattgg tgggattata attattatca tgggattatt
    24061 ttacatggga ataattaaat cttcaatatt gaatagagaa aaaagattta atgttaagtt
    24121 taaagaaatg aaagcaataa cagcttttat attaggtttt acatttagtt ttggatggac
    24181 accttgtatt ggtccaatat tggcatctgt attagtcatg gtttcaagtt caagtaatca
    24241 tcttagtgcg aatcttttaa tagcagtata tactattggt tttattttgc cattcataat
    24301 tacagctatg ttttacagta aactatttaa aactatagat aagataaaat ctaacatgga
    24361 aattattaaa aaaataggtg ggattatact tatagtatca ggaatattaa tgatggtaaa
    24421 tggatttgga agtataagca aacactttaa tacgtcacaa aatagtaaaa ttgaaagcaa
    24481 gcaagaagaa aataagcgag aaaattctac tgataaagaa gaaaattctg atggtaatga
    24541 tagtcaaaaa gactctaata atgacaataa tgataaaggg tctaatgatg aagatagaat
    24601 aaagtctatt gattttactt taacagacca gtatggtaaa acacataaat taagtgatta
    24661 tgaaggcaaa gttgtattct taaatttttg ggctacatgg tgtcctccat gtaaagaaga
    24721 aatgccttat atagaacaac tgtataaaga ctataataaa aataatgatg atgtagtaat
    24781 tttaggagta gcttcaccta atttaggtag agaaggttca agggaacatg tagttaactt
    24841 cttaaaagac caaggttata cattccctgt tgtacttgat gaagatggag cattagcata
    24901 tcaatatgga attaatgctt ttccaactac atttataatt gacaaggaag gatatgtaac
    24961 tcagtatatt ccaggagcta tggataaggc aactatggca tcatttattg aaaatcagag
    25021 aaataaataa aagtatttaa gtataagttt ataaatcaat ttaaggttac aaaaaagtgc
    25081 ctaattgtac cacctaaata atcttgttat agtaaaagta taaacaatac aagaaagggt
    25141 tgaattttat atgaaaaaaa catttgatgc tataataata ggatttggaa aaggtggaaa
    25201 aacattagca ggggatttgg caaacagggg attaaaagta gctttaatag aaaagtcaaa
    25261 caagatgtat ggtggaacat gtgtaaatgt ggcatgtata ccaacaaaaa gcttagaaaa
    25321 ttcagcaaac agcgtaaaaa caaagaatat aaattcttgg gatgaagtac aagctgaata
    25381 tgagaaagct atagataaaa aagaaacttt aattacaaaa ctcagagaag ctaactataa
    25441 taaattaaat tcaaatgaga atgtaactat attcacagga atgggaactt tcattgatga
    25501 aaaaacagtt caagtcaaaa ctgaaaatga aatttatgag ttagtagcag ataatatatt
    25561 tattaatact ggttcaagac catttatacc taatattaag ggaattgaaa ataagaatat
    25621 agtatatgat agtgagtctc ttatgaatct aagaacttta cctaaaaaaa tgacaattat
    25681 aggagctgga tttataggtt tagaatttgc tggtatttat agttcatttg gagcagaagt
    25741 tacaatatta aactctaata atggcatttt accaaatgaa gatgttgagg attcagaaga
    25801 aataataaaa ttacttgcaa agagaaatgt aaaaattgtt aataatgcaa atataaaaga
    25861 gattaaagaa gtttctgaat tagcaatagt agaatatgaa gtagatggta aatctaaaga
    25921 attaacaagt aatatgatac ttgtagctac aggaagaaaa gcaaatactg aggagctagg
    25981 gctagaaaat gcaggtatag aattaaatga aagaggattt attaaagtct cagaaactct
    26041 taagactaat aaagagcata tatgggctat aggagatata aatggaggtc cacaatttac
    26101 ttatatctca ttggatgatt atcgcatagt tattaatcag ttgtttggag ataagactag
    26161 aacaactaat gatagaaaga atatcccaaa ttctatattt ataagtccag cattttcaag
    26221 agttggtctt aatgttaaac aagctaaaga aaagggatat gaagtattag tggcaaagat
    26281 gccagtagaa gcaattccaa gagctaaaca gattggcaag gcagatgggt ttataaaaat
    26341 agtgatagat aaaaaatcca ataagatact tggagctagt atgatatgtg aaaattcaag
    26401 tgagattatc catcttattc aattagctgt agatttagaa gtagagtata catatttaag
    26461 agaccgtgtt tatgcacatc caactatgac agaagctctt aatgatattc tatctcctaa
    26521 tatgataaaa gaagtataac ataagtttaa tatataaacc taaaattatc attataagta
    26581 gattttagaa agaggctatc tcaaaaaata gagataagtc tcttttttac tgtttaaatg
    26641 ataaaaatat atttgatttt ataaaagaag attatcttat gaactaaaaa gtttatttta
    26701 aaacactacc tatttatgtt ataaacttat aaatataata tagaatctat tattctgtat
    26761 tattaagtgg ggttaagcgt tataattaat tataaatatt gaataatcta aatggataag
    26821 ataatggtat taaaaattat cttaaaaggt gaaataatct gtatttaatg tagtacagtt
    26881 atatctttaa agcaaggtgg tgaaatgata taaataagat atctaaaaga gggtttgatg
    26941 atagtgataa aagatggttt tcaagtaaag aattaacaag acttataaag tctaaagaag
    27001 aaatagaatg gttattaaat agggattaca aaatagaccc agtcgttact tttgttggtg
    27061 atagatatca gttttctata agacaaagag atgcattaaa aagggcagtc tgtactgatg
    27121 aaaaaaatat tataagacaa tcaaaaaggc tttctctaga taaaatcaaa gaagataccg
    27181 taaatataga tggctttaat ttgataatat ccatagaggt agcattatct ggtggtactc
    27241 tagtaatagg aagtgatggc aatataagag atttagcagg tcttagggga acctataaga
    27301 ttatagataa aacagaagaa gctttgaatc tgataggtaa attttttaat aaatacaatg
    27361 cacagaatct taaattttat ttagatgctc cagtttctaa ttcaggaaat ttaaagtata
    27421 ggatattaga gcatgccaaa acttggggaa ttgaaactga agtagaatta gtaaaaaatg
    27481 cagatgttgt attggaaaaa ttagatagag tagttagtag tgatgcagtc atagttgaca
    27541 agtgtataag ttattttaat gtggctagag gtataattga agagtatatt aaagaatgta
    27601 atatcataaa tttaaataaa tagataaata taggtgagaa tatataagta tatgtagcaa
    27661 tattcttagt aatacaagta aagtttgaaa ataggagaac gaatacatat cgaatttcat
    27721 aaaatgttgt aaaaagacat atgagaggat ggatatgtgt gaaattaact aaactaaata
    27781 ttaaaaaata tagagcacct atatataaat atgctttagc aaatgtaaat ttgaggtctg
    27841 ctaaatctac taattcaagt atcataacag taatacctca gggagccaaa atggaagtat
    27901 tggatgaaga agatgactgg attaaggtga tgtacaattc tcaagaagga tatgtgtata
    27961 aggatttggt atcagtaagc gaatatgctt ggagtaactt aaacttaaga gaagataaat
    28021 ctacaacttc caatataatt acagtaattc ctgaaaaatc tagggttgaa gtccttcaag
    28081 tagatggaga ttggagtaag gttgtatatg atgataaaat aggatatgtt tttaattatt
    28141 ttttatcaat tgatgggaat aaaccaaatg aattagatta taagtatttt tatactgaca
    28201 tgattaaatt tgttaacgaa aataacatta aaagtactag cgattatcta attgtaacag
    28261 accttagaaa taaatatact tatattttta agaaagataa tggaggatgg ggacaacttt
    28321 acaagtggca atgtactata ggcaagcctg agactccaac tataacaggt atattttata
    28381 taagtggaag aaaaccttct tttggaacgg atgagtattc agttaagtat gctactagaa
    28441 ttaaaggtgg atattactat cattctgtac tatatgattc aacaggaagt tatataatag
    28501 atggcagact tggtgaagct cttagtcatg gatgtataag attaagtacg gaaaatgcaa
    28561 agtggatata tgataatata ccagatacaa caacagttat aattcattaa gaaaaatttt
    28621 gagtttttta ttgggataat aattcttaaa ttaatagaat tatatattaa cttctcataa
    28681 agagtactat ttaaaaagtg taataagaga gtgctattaa caaaacaaag atattttgag
    28741 gcactctctt ataaatatac ataattaaga tattttcata aacaattcta taatcttatc
    28801 tttatcaaag aaaacaggat tagcaccagc acaagcatct ttcatagagt tttctgcaag
    28861 caattcaaaa ttaggattat caacacctac atcttttaaa gattttggga ttccaacctc
    28921 ttcagaaaga gcttttatct tatctattac aaagtcaaca cactctttat ctgatttatt
    28981 atttacatcc attccaataa cttcagctat tggtcggaat ttggcagggg ctgatttagc
    29041 attttcctcc tcaactattg gaagtagcat ggcattacat actccatgag gtaaatcata
    29101 taaacctcct aattgatgag ccatagcatg tacatttcca agacctgcat tactaaaggc
    29161 aattccattt aaaaaacaag cataacacat ttgttctcta gcttctatgt cattaccatt
    29221 tttaacagct cttggaaggt atttaaatat ctgctttata gcatataagg cagttgaatc
    29281 tgtaacatca taagcaccat tagctactac agcttctata gcatgagtta gtgcatccat
    29341 accagttgct gctgttaaat tagatggttt gctaatcatt aattctgggt catttactgt
    29401 cattgtggct agtgaattgg tatcaaccat tatcatcttt acatgtctgt cttcatcagt
    29461 tataacatag tttattgtaa cttcagcaga tgtaccagca gtagtagtta tagctatgat
    29521 aggaagacat tttttagaag ttttgttgat accttcatag tctctaatat ctcctccatt
    29581 agtggctaat atagagatag ctttaccaca gtcttgtgga gaaccacctc ctatagttat
    29641 aacgaagtcg caattttctg attttaatat tttaagtcct ttattcacat tagttacagt
    29701 tggattttgt ttaacttcat cataaacaac aaagtctaca tctatctgag ataaaatatc
    29761 tgttactttt tttacagtac catttgaaac tagaaattta tcactaacaa taaatgcttt
    29821 tttagttcct aatgatttaa ttgaatcttt agcttcatta agacatcctt tcccaagtaa
    29881 atttattggt ggtacataat aaatgctcat aactaaaatc tccttttatt ttcattgatt
    29941 tttatgtaaa aatatatatc taacaatttt cactaactta ctcataatat atcgtatctt
    30001 agattgagat aggattgttg gttcttatat aagaacttaa ttcgtaatta cgattatgat
    30061 tagaatttaa catagtattt ttatcatgtc aataaaaata taaaaaatat tataaaaaaa
    30121 acaaagttac tgtagttaaa tcagcaactt tgtttttata gtataaatca aataaataca
    30181 ttagtttaaa acaaaatttt taattattat gttttgatat cttccatata cagaatatta
    30241 atctaattta ttacaatgtc ttcattacgt tcttccatca ttgattgaat atttttctga
    30301 gcatttaaga gtaatttttt tatcagttct attttttcgt cattagctct aaataaaggc
    30361 agtgttacac taacagcagc tcttatacat ccattttttc ttattggtac ggcaagacat
    30421 tttaggtgtt ccattgattc ctcacactct gaagctattt gtgttctttg aacttcaaga
    30481 agctggtcgt ataaaacatc aaaatctgta attgattttt ctgtaaaacc ttttagacca
    30541 tctggatata agcttattag tttttccttg tcaaaatcac ataaaagagc ttttccaaga
    30601 cctgtacaat tagcaggtat tttttttcca acataagaaa taagtctaat tggttctgga
    30661 gaatctactt ttccaatata aagaacttca ttattgtcaa gtataccaag ctgacaagtt
    30721 tcagaacagg aattaactat attagtcatt tcgtcaatta tatagttaaa aatagttctt
    30781 ttttcaaaaa atgaagaccc taatgtgtaa gaacgtatcc ctatagaata tttggatgtg
    30841 tttttatcaa tagtaatgta ttttctagca ttaagtgtat gaactatagg aaagatgcta
    30901 ctttttggag cattgataac atctgctatt tctgtaagag tatatccatt ttcggattct
    30961 gctaatgctt ctaaaatatc taatatccta gctgttgggc gatgattatc tgaagtcata
    31021 aatttccccc tctttttatt aatttaaagt attagaatat gttgatttta gcacactata
    31081 cgatatttta caatgtagaa aataaatatg taaatttata tataaataag ctcttgattt
    31141 agatacagat agatattttt ttagactgta cattaaggaa taactaattt ccaaaatgta
    31201 cctaaagaaa gtaagtgata tttagttata ta
//
LOCUS       DAYAOY010000047        31017 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYAOY010000047 DAYAOY010000000
VERSION     DAYAOY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.047"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..189)
                     /locus_tag="ACGI6B_002158"
     CDS             complement(<1..189)
                     /locus_tag="ACGI6B_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9751601.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILIS"
     gene            373..717
                     /locus_tag="ACGI6B_002159"
     CDS             373..717
                     /locus_tag="ACGI6B_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFG9751602.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            748..2349
                     /locus_tag="ACGI6B_002160"
     CDS             748..2349
                     /locus_tag="ACGI6B_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFG9751603.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2368..3468
                     /gene="dprA"
                     /locus_tag="ACGI6B_002161"
     CDS             2368..3468
                     /gene="dprA"
                     /locus_tag="ACGI6B_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFG9751604.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3501..5588
                     /gene="topA"
                     /locus_tag="ACGI6B_002162"
     CDS             3501..5588
                     /gene="topA"
                     /locus_tag="ACGI6B_002162"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFG9751605.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5753..6538
                     /gene="codY"
                     /locus_tag="ACGI6B_002163"
     CDS             5753..6538
                     /gene="codY"
                     /locus_tag="ACGI6B_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFG9751606.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6645..7910
                     /locus_tag="ACGI6B_002164"
     CDS             6645..7910
                     /locus_tag="ACGI6B_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFG9751607.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7941..8711
                     /locus_tag="ACGI6B_002165"
     CDS             7941..8711
                     /locus_tag="ACGI6B_002165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9751608.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8820..9266
                     /locus_tag="ACGI6B_002166"
     CDS             8820..9266
                     /locus_tag="ACGI6B_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFG9751609.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9284..10477
                     /gene="nifS"
                     /locus_tag="ACGI6B_002167"
     CDS             9284..10477
                     /gene="nifS"
                     /locus_tag="ACGI6B_002167"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HFG9751610.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10531..10971
                     /gene="nifU"
                     /locus_tag="ACGI6B_002168"
     CDS             10531..10971
                     /gene="nifU"
                     /locus_tag="ACGI6B_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HFG9751611.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11260..12339
                     /gene="mnmA"
                     /locus_tag="ACGI6B_002169"
     CDS             11260..12339
                     /gene="mnmA"
                     /locus_tag="ACGI6B_002169"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFG9751612.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12482..12720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12788..13372
                     /locus_tag="ACGI6B_002170"
                     /pseudo
     CDS             12788..13372
                     /locus_tag="ACGI6B_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
     gene            14118..14387
                     /locus_tag="ACGI6B_002171"
     CDS             14118..14387
                     /locus_tag="ACGI6B_002171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751613.1"
                     /translation="MQDTMVQNNVRYICNSATNSNTTESKIDQDIIDQEGKTQLGFGD
                     NHRGCITIGSSEYIYTRRLKDPFQVQKGENPYAYTLERVDKSFNI"
     gene            14655..16496
                     /gene="ltrA"
                     /locus_tag="ACGI6B_002172"
     CDS             14655..16496
                     /gene="ltrA"
                     /locus_tag="ACGI6B_002172"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFG9751614.1"
                     /translation="MYNTETKAILNKDLKPLKKDMLRFNEYYNMQHIFDNLYKQSQQG
                     SKFSNLYNIIVSNENILLAFRNIKTNAGSRTPGINRHNIKYWKEKPTEEYLQYIKRRL
                     ENYRPQKVRRVEIPKANGKMRPLGIPCIEDRLIQQCIKQVLEPICEAKFHPHSYGFRP
                     NRSAEHAIAYMMKKINLEKMYMAIDIDIKSFFDNVNHSKLLKQMWSIGIRDKKVLSII
                     SHMLKAKVENVGIPDKGVPQGGILSPLLSNIVLNELDWWISNQWQTFKTKYPYMHSNH
                     KYRALRRTNLKEMYIVRYADDFKILCKNRNQAKRIFTATNNWLKERLGLEISEEKSKI
                     TDIRYSNSEFLGFKLKAYKKKKKYVIQSSMTDKAKQQTISNIKEQYEVIKHYPINKNI
                     YTLNRIIAGSHNYYKIATNVYRDFSNIQYNLSKCYKNRLDNLVTKQGAVTQEYLNKYM
                     QYGIKEKYIADAVIYPLTGIKTTMPHVFNQKVTNYTNEGRQIIHSTLGYIDHEMLNYI
                     AKNPIPNRSVEYNDNRLSRYSAQQGICQISKRLLNEDMECHHVIPRSLGGDDRYRNLI
                     LILPFVHKLIHATKQETINFYLEELDLNKQDIKRVNNYRVKVGNELI"
     gene            16572..18653
                     /gene="alaS"
                     /locus_tag="ACGI6B_002173"
     CDS             16572..18653
                     /gene="alaS"
                     /locus_tag="ACGI6B_002173"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFG9751615.1"
                     /translation="MTSNSYLSTYLEFWNHVFTQFDRDEEGNYSLLENKNIDTGMGLE
                     RMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRIITDHIRAVTFLVS
                     DGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVNDKAYPVLVEKES
                     YIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFKLYDTYGFPIDLT
                     KEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSKLESTVDSTFNGY
                     SEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVGDCGLITNENLVL
                     EVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNHSATHLLHKALRE
                     VLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILSSLDIKCDIMNIK
                     EAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMFKILSEGGVAAGV
                     RRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEENKNLSKELHDMK
                     AKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDKLGSGVVVLANVV
                     DDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAGASDVSKVDQALS
                     YASEVIKTQVK"
     gene            18678..18935
                     /locus_tag="ACGI6B_002174"
     CDS             18678..18935
                     /locus_tag="ACGI6B_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFG9751616.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            18945..21116
                     /locus_tag="ACGI6B_002175"
     CDS             18945..21116
                     /locus_tag="ACGI6B_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751617.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            21322..21747
                     /gene="ruvX"
                     /locus_tag="ACGI6B_002176"
     CDS             21322..21747
                     /gene="ruvX"
                     /locus_tag="ACGI6B_002176"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFG9751618.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            21825..22112
                     /locus_tag="ACGI6B_002177"
     CDS             21825..22112
                     /locus_tag="ACGI6B_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFG9751619.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            22394..22852
                     /locus_tag="ACGI6B_002178"
     CDS             22394..22852
                     /locus_tag="ACGI6B_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFG9751620.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(22969..23706)
                     /locus_tag="ACGI6B_002179"
     CDS             complement(22969..23706)
                     /locus_tag="ACGI6B_002179"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9751621.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            23923..25599
                     /locus_tag="ACGI6B_002180"
     CDS             23923..25599
                     /locus_tag="ACGI6B_002180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFG9751622.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            25805..26005
                     /locus_tag="ACGI6B_002181"
     CDS             25805..26005
                     /locus_tag="ACGI6B_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HFG9751623.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            26059..27222
                     /locus_tag="ACGI6B_002182"
     CDS             26059..27222
                     /locus_tag="ACGI6B_002182"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9751624.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(27433..27855)
                     /locus_tag="ACGI6B_002183"
     CDS             complement(27433..27855)
                     /locus_tag="ACGI6B_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HFG9751625.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(27876..28712)
                     /locus_tag="ACGI6B_002184"
     CDS             complement(27876..28712)
                     /locus_tag="ACGI6B_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFG9751626.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            29010..29606
                     /locus_tag="ACGI6B_002185"
     CDS             29010..29606
                     /locus_tag="ACGI6B_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFG9751627.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            29636..30355
                     /locus_tag="ACGI6B_002186"
     CDS             29636..30355
                     /locus_tag="ACGI6B_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFG9751628.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            30357..30896
                     /gene="scpB"
                     /locus_tag="ACGI6B_002187"
     CDS             30357..30896
                     /gene="scpB"
                     /locus_tag="ACGI6B_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFG9751629.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        12578 a         3359 c         5584 g         9496 t
ORIGIN      
        1 agaaataagt ataaatttat tctcatttga taaagaaaaa aatctaactt gattatttat
       61 tataattaga aataatatta tcatagttat gaccggatac aatgtatttt caacattttt
      121 caaatagctt acaactaata taaataaaga taaatatcgt ataaaagcaa ctaatttttc
      181 tctgttcatt aaaggcactc ctccaaaaaa ataaactttt cctatgaaaa ctagaataat
      241 atcataaaag aataaagtca atagaagtat ttattaatat tgatggaaat aaaatcaatc
      301 caaaaacttg aatagtaaaa ttgtatactg accataattt aaaagtgaaa atttaatgta
      361 ttggagtgaa taatgaataa taaagaaaaa ggtgattttg gagaaaaagt agcagttaat
      421 tatttgctat caaaaggagc aaagatacta gaaaaaaatt acagattaaa gataggggaa
      481 atagatataa tagcaaaaat ggaagatgaa attatatttg tggaggtgaa atcaaggtct
      541 aacataaagt ttggctaccc ttgtgaatct gtaagcttca aaaaaagaaa aaagataatt
      601 ggagttgcaa attattatat tataaaaaat aacttaaata acactcccat aagatttgat
      661 gttatagagg tatatttatt agaaaaaaga ataaatcaca taatgaatgc tttttaaaaa
      721 agataatgtt tttggaaagg attaaatatg ttaagtatta ttaattcaag caacttagta
      781 ggaatcgaca gctttttagt taaggttgaa gtagatgtaa gcaatggaat acctagtttt
      841 aatattgttg gtcttccagg caaagaaatt aaagaagcaa gagaaagagt aaagtcagca
      901 attcttaata gtggatataa atttccaagc acgagaatag ttgtaaattt atcaccagca
      961 gatataaaaa aagaaggagc ttttttagat ttatctataa gtataggcct tttaagagag
     1021 ttaataaaaa aagatgaaaa ttacataaga gaaagtatgt ttataggaga attatcatta
     1081 gatgggaaaa taagaaaagt aagaggtata ttgcccataa ttatgggtgc aaaaactcag
     1141 aacataaaac gtatttttat accaattgag aacataaaag aaagtctatt tgtagatgaa
     1201 attgatataa ttcctattaa aagtttaaaa gaatgtgtcg attttttaaa cgaagagata
     1261 aaagtcgata aagtaaggat aatgtctttt ttggatgata agtcaagaaa agagaacgaa
     1321 gaattagaaa aagataatag ttatatagat tgcaaatata ctaagattaa taatgaagaa
     1381 agtaaatatg atgaagactt taaagatgta aaaggaaatt attttgtaaa gaggagtgca
     1441 gaaattgctg ctgctggaaa tcataatatg tttatgattg gacctccagg ctcaggaaaa
     1501 actatgattg caaaaagagt aaggacaatt ttaccagata taagtgtaga agaaatgata
     1561 gaagtaagta aggtctatag tatattgggt atgataaatg aaactaaagg aataattgat
     1621 aaaagacctt ttagagcacc tcatcataca actacaaagc aatctttaat tggaggagga
     1681 atggatgcaa gacctggaga aattgcttta gcacatagag gtatcttatt tttagatgag
     1741 atagccgaat ttgatagaaa aatacttgag actttaagac aacctataga agatggatat
     1801 gtaaatatat ccagagtaaa gtacagtgca aaatatcctt gtagagtact gttagttgca
     1861 gctatgaatc catgtccatg tggttattat atgtcagaga cagagtgtag atgtagaagt
     1921 aacgaaattg atagatacat aaataaaata tcaggtccat tgttagatag gtttgatatt
     1981 tttgtagaag ttaattctat aaaatattca gattttaatt ctttaaaaca agaagaaagc
     2041 tctcaaaaaa taaagagaag agtagaaaat gctagaaaaa tacagataaa tagatttaag
     2101 aaagataata taaaaaataa tagtgaaatt aaagcttaca atttatttaa atattgtaaa
     2161 ctggaaaaag aagcttcaaa aacggctgag atgatattta gtaaatacaa cctaagcagt
     2221 agaagttata caaaactttt aaaaatggca agaacaatag cagatttaga agaaagagat
     2281 ttgataaatt cacagtgtat tatagaagct ttttcattta gaaaggctta ttatagttat
     2341 tttaaatagt tttttgaggt ggattttatg gaaaaaagag atacttattt atggttaaaa
     2401 tcaattggtg gaataacaac aaaaacaatt gaaataatag aaaatgaaat agtaaatatt
     2461 gaagatatat ttgatttttc tgaaaaagaa atatataatt taaaaaatat aagcttgaat
     2521 attagaaaaa atatagtaaa atatagaggt cacgcttatt tagaaaatat aaaagaacta
     2581 ctttacaaaa aagcgattaa atatatttgt aaatatgata aagaatatcc tgaaaattta
     2641 aagaatatat acaacgctcc aaagttatta ttctacaaag gtgacatagg cttagttaat
     2701 aataatttta atatagctat agtaggttca agaaaaccta cagcctatgg aattaattgt
     2761 gcaaaaacaa taagttgtca actatcccaa tatggagtta atatagtaag tggattagct
     2821 ataggaatag atgcatactc gcacataggt tgtatgagtg gaaaatctaa aactatagca
     2881 gtacttggtt caggagtaga caatcctctt ccaaaacaaa atttacattt atcaaataaa
     2941 atattagaaa atgggggatt attattatca gagtataata ttaactcgac agtagccccg
     3001 taccattttt ctaatagaaa tagaattatc agtggtttaa gtgatggagt agttgttgta
     3061 gaagcagcaa taaaaagtgg tgcactaatc acagtagatt ttgctcttga acatggaaaa
     3121 aatgtttttg caatacctgg taatataaat tctcaaatga gtagaggttg tcataaaatt
     3181 ataaaagaag gtgcaaaatt aattgaaaat atagatgata ttttaaatga atataacata
     3241 tttaatatta ttgataaaaa aataaatcaa aaatatgata atataagtct aaatgccaag
     3301 tctaagcaaa taattgaagc tataaaaaga gaaggaaact tgcatataga tagtatttgt
     3361 gattatactg gtatagaaat aaagtatgta aattcaataa tcaatgaatt agtactaaat
     3421 gagttagtag tagaaatgaa taataaaacg tacagtttaa atgtataaat taagattagg
     3481 tagtgggggt gtatggtttt atggccaaaa cattagtcat cgtagaatcg cctgcaaaag
     3541 ctaaaactat agaaaaattt ttagggaaaa gtcattacac agttaaagca tcagttggtc
     3601 atgtcagaga tttacctaaa agtaaattag gtgtagatat agaaaataat tttgaacctc
     3661 aatatataaa tataagagga aaaggcgatg taataaaaga attaaaaaaa gaagctaaaa
     3721 aatctaaaca agtatatcta gcaactgacc ctgatagaga aggtgaagct atatcatggc
     3781 atttagctca tatactaaat ttagatgaat ctgaggattg tagaatagag tttaatgaga
     3841 taactaagga tgcaattaaa aaagcaatta aacatcctag aaatattgat ataaatttgg
     3901 ttgatgcaca acaggcaaga agagtgctag acagactttt aggttatcaa ataagtccta
     3961 tattatggca aaaggttaga aaaggtctta gtgcaggtag agtgcaatct gttacaacaa
     4021 agttaatttg tgatagagaa aaagaaataa aggcatttat tccaaaagaa tactggacaa
     4081 tagatgttga ggctaagaca gtaaaagaca aagatataac tttgaaattt tatggtaaaa
     4141 atgatgaaaa gattgaatta gaaaatgaag aagctgtaaa tgaaatacta aaagaaatgg
     4201 aaggtaaaga tttaaaggtt gagaagatag aatctaaatc aagacgtaaa tcagctccaa
     4261 aaccatttac aacaagtatg cttcaacaag aaggagcaaa taagttattt tttactacta
     4321 aaaaaacaat gatgatagca caagaattat atgaaggtat cgacatagaa ggtgaaggta
     4381 ctgttggtct tatatcatat ataagaactg actctaaaag aatatctgat gaagcaaaag
     4441 aaaaagctaa gggttatatc gtaaacaatt taggagaaaa ctactataaa aacattggtg
     4501 ataaaaaaga aaaaaaggat actaaaaagg ttcaagatgc ccatgaggca ataagaccaa
     4561 cctcagttga tagaactcct gagagtataa aagcctcatt gagcaaagac cagtacaagt
     4621 tatacaattt aatttggaga agatttgtag ctagtcaaat ggaagattca gtgtttgata
     4681 tactaaatgt agaatgtaaa ataggcgata ttgtatttaa ggcaactggt tcaaaaatga
     4741 aatttgatgg ttataccaag gtatataatt tttcagaaag agaagacaaa attttaccaa
     4801 gtatagaaaa aggagatata ttaaatatag aaaatatatt gccagaccaa cattttacac
     4861 aaccaccagc aagatataca gaagctagtt tagtaaaaac actagaagaa ctaggtatag
     4921 gtagaccaag tacgtatgct ccaacaatag ctactatact gaatagagag tatgttgaaa
     4981 agcaaggaac aagtctatgt ccaacagaac ttggaataat agttactgat attttggaaa
     5041 ataatttcca taaatttata gatgtagatt ttactgctga tatggaaagt aaacttgatg
     5101 ttatagaaga aggcaataca gattggaaaa agattgtatc agaatcatat gcaccactta
     5161 aagaggctat tgaagaagct cttgaaaata tagaaaaagt aaatatggat gaagaaactg
     5221 atgagatttg cgagaactgt ggttctaata tggttatcaa atatggtaga tttggaaagt
     5281 ttatggcttg taaaaactat cccgattgta aaacaactag accaattatg aataaaattg
     5341 gtgttaaatg tccaaaatgt gaagaaggag atattatact tagaaaatct aaaagaggta
     5401 aagcttttta tgggtgttct aattatccaa actgtgactt tgtagcttgg aataagccaa
     5461 caggagagtt ttgtgagaag tgtggttcat atatggttga aaaagtgact aaatctgaaa
     5521 caaaaacagt gtgttctaat aaagaatgta ataaagagac aaaagagcat aatgacggaa
     5581 aagagtgaaa attgaaaaaa atcacataat tctcttgaaa aaggttccaa tctatgataa
     5641 tctttcaagt gtaaacaact aatagaaaaa taaattttca ttaaatgtga aaataatatt
     5701 agaaaatata aaaggttatt aaaaggccta aatttcatga ggagatgatt aaatggcaag
     5761 tgaagtgtta caaaaaacaa ggaaaataaa taaaacatta caaacaagtg gtggaagcag
     5821 tgtctctttt gatttactgg ccggagcatt gggcgacgtt ttaagttcta atgtttatgt
     5881 agtaagtgca aaaggtaaag tactaggtct tcatttaaat gatgttcaag acagttcagt
     5941 tatagaagat gagtatacta agcaaaagaa attttcagat gaatatactc aaaatgtgtt
     6001 aaaaattgat gaaacattag aaaatttaaa tggtgagaag atattagaaa tctttcctga
     6061 agaacatgga agattacaaa aatatactac agtagttcca atattaggaa gcggtcaaag
     6121 attaggaaca ttggtacttt caagatattc aaattcattc aatgatgatg atttagtaat
     6181 agctgaatac agtgcaactg ttgttggtct tgaaatatta agagcaatag gtgaggaatt
     6241 ggaagaagaa atgagaaaga aagctgtagt tcaaatggca ataggcactc tgtcctactc
     6301 cgagcttgaa gcagttgaac atatttttgc tgaattggat ggaaaagaag gtttacttgt
     6361 agcaagtaag atagctgata gagttggtat aactaggtct gtaatagtaa atgcacttag
     6421 aaaatttgag agtgcaggtg tgatagaatc aagatcatta ggtatgaaag gtactcatat
     6481 aagaatactt aatgacaaac ttacagatga attaaaaaaa ttaaaaaaca atcaataatc
     6541 tatattttat aggtttagat tagaaaaggt atctatttca aatagatacc ttttttagac
     6601 gaattattaa ggtaatgata agtatattaa aaaggagtga gaaaatgcct ttagcagata
     6661 aaataagacc aaaaacaatg aatgatgtaa taggtcaaag tcatataata ggtaatggaa
     6721 aaatattgtc taagatatta caatctaatt ttttaccaaa tatgatattt tttggtcctc
     6781 ctggtgttgg taaaactaca gtagcggaaa taatagcaga aagaagcaat aaaagcttct
     6841 ataaaataaa tgctactaat tcatctttag aagatataaa aaaggtgata tcagagcttg
     6901 gaagtataaa taatgtaaat ggagtcctat tatatataga tgaaatacaa agttttaata
     6961 aaaaacagca acaatcaata ttagaattta tggaaaatgg gagtataact ttgattgcta
     7021 gcactacaga aaatccatat cattatgtat ataaagctct tttaagtagg tctactgtat
     7081 ttgaatttaa acctttagaa aaacttgata tagaaaaagg actgaaaaga gctgttgaag
     7141 tattaaatga agatagctat atggatattg agtgtaataa tgatgctatt gaagatatag
     7201 caattctttc tgatggagat atgagaagag ccttaaatat tttggaagtg gtagtctatt
     7261 caactaaacc aaataaggat aatataactt atatagattc agatgtaata agggcatcta
     7321 catttaataa aattataaac tatgacaaaa atggtgatag tcattatgat attttaagtg
     7381 catttcaaaa atctataaga ggaagtgacc cacaggcatc tatacactat ctagcaagac
     7441 ttataaaagg tggggatttg ataagtatat gtaggagact tttagtaata gctagtgaag
     7501 atataggttt agcttatcca aatgctattg taattgtaaa ggcatgtgta gactcagcta
     7561 tgcaacttgg atttccagaa gcgaaaatac cattagcaga agctactatt cttttggcta
     7621 catctccaaa gtctaattct gcatgtatag caataatgaa agctatggat gaactggata
     7681 aagagtttgt taaggatata ccaaatagta taaaggatgc tcactattct ggttctaaag
     7741 aaataggtag gggttgtgga tacaaatatc ctcataattt taaaaatcat tatgtaaagc
     7801 aacagtattt accagatagc ataaaagata gtatttatta cataccacaa gaaaataaaa
     7861 ctgagaaaaa tataaaaaaa tatttagaat atctaaatag tgatttttag actgaacttt
     7921 atgtatcggg ggtagatatt atgattataa aacttaactc aacttatcac agtagagtta
     7981 tgaaatactt aaaaaaagaa cctgagtaca atttgtttat aataggggat attgaaagat
     8041 atggatatgg aaataatttt ttaaatatat gggcagatgt gggtgaacat ggtgaaataa
     8101 aggcaatatt acttaaatat tttgaattta tgatgtttta ttctgatgga gagtatgatg
     8161 ttgaaggttt ttataatcta cttcgtaata ctaattacga agaaatatct gggaaaatat
     8221 gtgctgtaga tgctttagca aagagattag gacttaataa tttaaaggta gttgattttt
     8281 gcaaacttca aactaaaaag tttttgatag ataataattg taatgcaaag gttaagagaa
     8341 ttaggcttgg aaatctaaaa aagacagtaa aactttatga cttaatagat gaatttcaca
     8401 gtacaacttt agagaattta aaaaatggac taaggacagg tagaggatat tgtatagaga
     8461 ttaacaaaca ggtggtgtca atggcaaaga gtacttcaga aaataggact catgcaatga
     8521 taattggagt aggaacacat cctaaatata gagctaaagg acttgctaca aaatgcctaa
     8581 ttaaactatg ttcagagtta ctgagagaaa acaaaattcc ttgtttgttt tatgataatg
     8641 aagaggctgg aaagatatat aaaaaactag gatttgaaaa cattggaaaa tggggaattt
     8701 attcaaaata aaaaattaaa attaatcttg acaaaattag tagggtataa taaaattatc
     8761 ttgaaagtaa ttcctactaa aacagtatga attagatttc tatatagagg tgataatata
     8821 tgaagttgtc tactaaaggt agatatggac taaaagcaat gtttgaatta gcattaaatc
     8881 aagacaatgg accagtatca ttaaaattta ttgctaagaa gcaaaaaata tcagatcagt
     8941 acttagaaca aatattttca tctttaaaaa aatcgggtct agtaaaaagt gtgagaggtg
     9001 ctcaaggagg ttatttgctt tcaaagaatg ctgaagacat aacagtagga gatatacttg
     9061 tagtattaga aggaccagtt gcattatctg actgtgtact agatgaagat gtatgtgaaa
     9121 actcaaacat gtgtgtaaca aaaatagttt gggaaaagat gaaaaagggt atagaagatg
     9181 ttatagattc aattactctt aaagatatga taaatgacta taacaaaaat aaattagaaa
     9241 atgatataac aaatattaaa aaatagattt ggagatgaat gtaatggaaa aaagaagatt
     9301 gtatatggat tattctgcaa caacacctat taaaaaagaa gtattggatg caatgatgcc
     9361 atacctaaca gattattttg gtaatgcatc tagttttcac acattcggaa gagaagcaaa
     9421 agatgctttg gataaagcaa gagaacaagt agcagccctt ataaatgctg aacctagtga
     9481 aatatatttt acagcaggtg gttcagaaag tgataactgg acactagagg gtgtagctta
     9541 tgctaataaa aacaaaggta atcatataat aacttcaaaa atagagcacc atgcaatact
     9601 tcatacatgt gaatacttag caaaacatca tggatttgaa ataacttatt tagatgttga
     9661 tagcgaagga aaagtagact taaagcaact agaagattca ataaaagata ctactatact
     9721 tataagtata atgtttgcaa ataatgaaat aggaactata cagcctatta aagaaatatc
     9781 cgaaatagct aaaaaacata agatattatt ccacactgat gcagtacaag ctacaggtaa
     9841 tattccagta gatgttaaag aattaggaat agatttgatg agtatgtctt cacataaaat
     9901 atatggacca aaaggtgtag gagctttata cataagaaaa ggtgtaagac ttcataactt
     9961 tgtccatggt ggagcacaag aaaaaagcaa aagagctggt acagaaaata tacctgcaat
    10021 agttggatat ggaaaagcag cagaattagc taaggaaaat atgcaaaatc atgtagaaac
    10081 tttaactcgt cttagaaata agttaataga tggtgtgtta gaaagaatac cttacacaag
    10141 agtaaatggt agcttagaaa atagattgcc aggaaatgca aactttgcat tccaatttat
    10201 agaaggtgaa ggaatacttt tattattaga tatgttagga attgctggtt caagtggttc
    10261 tgcatgtact tctggttcat tagacccttc acatgtactt cttgcaatag gtttaccaca
    10321 tgaaatagca catggttcat taagacttac agttggagat tttacaacag atgatgatat
    10381 agattatata ctagagaact taccaaaagt aatagaaaga cttagaagca tgtcaccact
    10441 ttatgatgat gcgaagaaac aaggtttagt aaaatagaca taaagataaa gcataaaaaa
    10501 tagacataaa ttgagtagga ggaaaagata atgcaatata gtgataaagt tatggaacat
    10561 tttatgaatc caagaaatat gggagaaatt gataatgcaa gtggtgtagg tgaagttgga
    10621 aaccctacat gtggagatat aatgaagata tttttagaca tagatggaga tgtaataaag
    10681 gatgttaagt ttaaaacatt tggctgtggt tcagctatag caagttcttc tatggcaact
    10741 gaaatgataa aaggaaaaac tataaaagat gcattagaac ttacaaataa agctgttgca
    10801 gaagctttgg atggtttacc accagtaaag atgcattgtt cagttttagc tgaacaagct
    10861 gtaaaagcag ctcttataga ttatgctcaa aagaacaata tccatatacc agaattggat
    10921 ggatatgtta tagatgatgc tcatgaccat gatgttgaag aagaagaata aaaataagtg
    10981 attgtgttat gagaagttta caatgattaa aaagaagtac taatgatata attaggacat
    11041 aaaaaatagc tgtttttaaa atagctattt tttattgcct aaattaagta ttttaacttg
    11101 atttagaggt taattagtta acaaaataat ccataagatt caaataataa agatgattat
    11161 ttttttagaa aatagaacaa gctatatttt aaataagtac aatacttgct ctatatggta
    11221 taataataag taatgcaaaa gatgaggtga aatttgttta tgaataaaaa agtaatgata
    11281 ggtatgagtg gaggagttga tagctctgta gctgcatatc ttttaaaaca acaaggatat
    11341 gatgtcattg gtgttactat gaagttgtgg caagatgatg atgttgttga aattgaaggt
    11401 ggttgttgct ctttatctgc tgttgaagat gctagaaggg tagcaaataa aataggtata
    11461 ccattttatg ttttaaattt tagagaagtg tttaaagaaa aagtaataga ttattttata
    11521 gatgaatatt tggaaggaaa aactcctaat ccctgtatag cttgtaataa acatataaaa
    11581 tttgatgatt tttataagaa ggctagacaa ataggttgtg attatgtagc tacaggtcac
    11641 tatgcaaaga tagaaaagga tgaaagtaca ggaagatatt tgctaaaaaa atctgttaca
    11701 gataaaaaag accaaactta tgcactttat aatcttacac aagagcaact agagcatact
    11761 ttattgccaa taggtgacta tgaaaaagat agagtaagag aaattgctaa ggaaatgggt
    11821 atggcagtac acaataagcc tgatagtcaa gaaatttgtt ttgttaaaga taatgactat
    11881 gcaaattatg taaaaaaaca ttctaaaaag cgtattgaag aaggtttttt tgtagatact
    11941 aaaggcaata tacttggaaa acataagggt atattatatt atactatagg ccaaagaaaa
    12001 gggcttggaa ttacttttgg aaaaccaatg tttgtaatag acataaatcc aataaataat
    12061 accatagttc ttggagacaa tgaagattta tttaaaaagg aacttattgc taaagatgtt
    12121 aactttatat caatagatac gttagaagaa cccttaagag ttcaagccaa aataagatat
    12181 tcagcaaaac catcaccagc tactattcat agagtaggag aagatactat taaaatagtt
    12241 tttgatgaag ctcaaagagc tattactaaa ggtcaatctg tagtaatgta tgatggagat
    12301 attgtagtag gtggaggtat aatcgagaaa agtttataaa aacatctaaa aaattaaaaa
    12361 aataagcaat aaagtatatt aagagtaaat aaagtttata aaataaattt attgtatatg
    12421 aaaatatagt attgattaat tttgtaaaat ttagtaatat aattagtata ttaattaaaa
    12481 aacaaataca gtgataagga atagtaaaat atttgatgtc ttacagagag agggaaatgc
    12541 tggaagccct tagaatatga tatttgaagc agccttttag tagcaatctt gaacaattta
    12601 tcttttataa agaagattta agtagagatt gacggtagtt acgttatagc taaggttttc
    12661 cgaagatatc atactttgta tgataattag ggtggtaccg cgattgtcct cgcccctata
    12721 aatttattta tagggacgag gttttttatt taaacgaaaa aataaaattt attaggagga
    12781 acatgatatg gaaaagatgg gattaaatga aataagaagt aagtttttag aattttttga
    12841 gtcaaaaggt cattatgtag caaatagtta ttctttagta ccaaacaatg ataagagttt
    12901 gttacttata aactcaggta tggcaccttt gaaaaactac ttttcaggtg tagaagtacc
    12961 tccaagtgtg agaatgtgta catctcaaaa atgtataaga acaggagata tagaaaatgt
    13021 aggtataact gcaagacatg ctacattctt tgaaatgatg ggtaatttct catttggaga
    13081 ttactttaaa agagagtcaa taaaatgggg atgggaattt gttacagaat ggctaaatat
    13141 accagaagat aagatatggg tgactgtata tgaagaagat gatgattctt atgatatatg
    13201 ggcaaaagaa atgaatttcc cagaagaaag aatggttaga cttggaaaag atgataattt
    13261 ctgggaaata ggtacaggtc cttgtggtcc ttgttcagaa atatattttg atagaggaga
    13321 agaatatgga tgtgacaatc cagactgtaa accaggctgt gattgtgata ggtgtgcgac
    13381 atgttgctaa ttgaaaagat tagccattaa cgaaccaaag aaggaggtat caaataaact
    13441 tctttaaatg gttaatagtt aatgaactga taacttgata tgtcaaatga tagagtaacg
    13501 tcttgaaggg caaccctaat actccgtttg gcttttgtat atgaacaacc ttgatacatt
    13561 agtcaaaagc acggtgaagt cggcagagat tatgtcctaa gactctatat gattcgctac
    13621 gtcgattgac taggttctac tggactgaac cttacttaaa gaatctatag agaaacagga
    13681 aatggggcta gaggtagtca agcataaata tatgccgggg gtctataaaa tacctatggt
    13741 tagaatgtat ccaaaagata ctgacgaatc tccgaatgta cagctctata cgcagacgac
    13801 atagaaatgt gttgggattc cattgagaat cgtctcagag gtttagtaag actatctgtt
    13861 atgaaaaacc ttatactgtt ataggcagta tcgttgggac agggttatag gagaaaccta
    13921 agggtatata ttaaaagata gagttatcgg aacgtggaaa gctaatgacg tggaggctat
    13981 tgttcagctt atgatacgtt aacaaggaaa ttggaagtat gaattttgaa tagtaataca
    14041 agttacaaaa cgacatttga ttccttacgt ttacctcacc taaaggtgaa atataaaacg
    14101 taaggaggtg atagaacatg caagatacaa tggtacaaaa caatgtaaga tatatatgca
    14161 attcagcaac aaatagcaat acaacagaat ctaagattga ccaagatatc atagaccaag
    14221 aaggtaaaac tcaactcggt tttggagaca atcatagagg atgtattaca attgggtcat
    14281 cagagtatat ttatacaaga agactcaaag accctttcca agtgcaaaaa ggagaaaatc
    14341 catacgcata tactttagaa agagtcgata aatcttttaa tatataactt cctcaaatat
    14401 aagttataag cttattaaaa gattaaagtt acaaataaca agacaaaatt caaaaaccat
    14461 ataacttgtt ttaacattag tgagagtaga gccacagtac cgttgaagcc atgataataa
    14521 gtggtggagg gataggctca agtcaaatag caaaagatta ttagattaat ttaaattcat
    14581 tagggttcac ggtatgacta agaaagatta tattccttac acattatata gaaaggaata
    14641 aatcgaccta atcaatgtat aatacggaaa cgaaggcaat acttaataaa gatttaaagc
    14701 cattaaagaa agatatgcta agatttaacg agtactacaa tatgcaacac atatttgata
    14761 atctatataa gcagagtcag caaggaagta aattcagcaa tttatacaat attatagtaa
    14821 gtaacgaaaa cattttgtta gcttttagaa atattaaaac taatgcagga agcagaacac
    14881 cagggattaa tagacataat attaaatatt ggaaagaaaa acctactgag gaatatcttc
    14941 aatatataaa gagaagacta gaaaactaca gacctcaaaa agtccgtaga gttgaaattc
    15001 caaaagctaa tgggaaaatg agacctctag gaataccttg tatagaagat agactcattc
    15061 aacaatgtat caagcaagtt ctagaaccaa tatgtgaagc aaagtttcat ccacatagtt
    15121 acggttttag accgaaccga agtgcagaac atgcaatagc atatatgatg aagaaaatta
    15181 atcttgaaaa gatgtatatg gctatagaca tagacattaa aagtttcttt gataatgtta
    15241 accatagtaa gttattaaaa cagatgtggt caataggaat tagagataag aaagttttaa
    15301 gcatcataag tcatatgcta aaagctaaag ttgaaaatgt aggaatacca gataaaggtg
    15361 tgccacaagg aggaatttta tctcctttac tatccaacat agtacttaat gaattagatt
    15421 ggtggattag taaccaatgg caaactttta aaactaaata tccctatatg cattcaaacc
    15481 ataaatatag agcattaaga agaactaacc taaaggaaat gtatatagta agatatgcag
    15541 atgattttaa aatattgtgt aagaatagaa accaagctaa acgaatcttt acagcaacca
    15601 ataattggtt aaaagaaaga ttaggactcg aaataagtga ggaaaaatcc aagataacag
    15661 atattagata ttccaattct gaatttctag ggtttaaact caaagcttat aaaaagaaaa
    15721 agaaatatgt gatacaatcc agcatgacag ataaagcgaa gcaacagact ataagtaata
    15781 taaaagagca atatgaagta ataaagcatt acccaattaa caaaaatatt tacacgctta
    15841 atcgaatcat tgcagggtcg cataattatt ataaaatagc aacaaatgta tatagggatt
    15901 tttccaatat acaatataat ctttctaaat gttataaaaa tcgcttagat aatctagtta
    15961 caaaacaagg tgcagttaca caagaatact tgaataaata catgcaatac ggtatcaagg
    16021 aaaaatatat agctgatgca gttatatatc ctttaacagg aatcaaaaca acaatgccac
    16081 acgtatttaa tcaaaaagtt acaaactata caaatgaagg acggcagatt atacactcaa
    16141 ctttgggata tatagaccat gaaatgttga attatatagc taagaatcca attccaaata
    16201 gaagtgtgga gtacaatgat aacagacttt cacggtattc agcacaacaa ggtatttgtc
    16261 aaatttccaa acgactctta aatgaggata tggaatgtca tcatgttata cctagaagct
    16321 taggtggtga cgatagatac aggaatttga tactcatact tccatttgta cataaattga
    16381 tacacgcaac aaaacaagaa acaattaact tctaccttga agaattagat ttgaataagc
    16441 aagatattaa gagagtcaac aattaccgtg tgaaagttgg aaatgaatta atttaattga
    16501 ttgcctattt gagggaacgc cgtatgaggt gaaagtctca cgtacggtgt gggtcggggg
    16561 aaaagacgga gattacatca aactcttacc tatcgacata tttagaattt tggaaccatg
    16621 tatttactca atttgataga gatgaagaag gaaattatag tctattagaa aacaagaata
    16681 tagatacagg tatgggtctt gaaagaatgg gatgtattat gcaaggcgta gacactatct
    16741 ttgaagttga tacaattaaa tctatactag aagcagttga gaagttaaca ggagtaaaat
    16801 atggagagaa tccaaaaaat gatatatcaa taagaataat aactgaccat ataagagctg
    16861 taacattctt agttagtgat ggagtgcttc catcaaatga aggaagagga tatgttctta
    16921 gaagactcct tagacgtgct gctcgtcatg gtaagttatt aggagtaaaa gaattatttt
    16981 tacagaaact aatagatgaa gttataaaag taaatgataa agcatatcca gttttagttg
    17041 aaaaagaaag ttatattaaa aaagtagttg gaatagaaga agaaaaattc aatgaaacta
    17101 tagaccaagg aacagaaata ttgaactctt atatagaagt attaaaaaat gaaggaaaga
    17161 ctgtcctaag tggtcaagaa gcttttaaac tatatgatac ttatggcttc cctatagact
    17221 taactaaaga aatattagaa gaagaacatc tatctgtaga tgaagaagct tttaatgaag
    17281 aaatggaaaa gcaaaaagaa agagctagaa atgctagagg aaatatggat ggagaaagtt
    17341 ggaaggaaga ccctctatca aaattagaat ctacagttga cagtacattt aatgggtata
    17401 gtgaaattta tggtgaagga actattgaag ctatagttaa agatgatgag ctagttcaaa
    17461 gtgcagaaga aggcgataag gtatctatag tactagataa cactactttc tatcctgaag
    17521 gtggaggaca agttggagat tgtggattga taactaatga aaacttagtt ttagaagtat
    17581 taaatactaa aaaaggtgct aataatagta taaaacatat aggtataata aaatcaggaa
    17641 gaataagtaa tggggacaaa gtgaagacac tcgtagatag agaaactaga atgtcagctg
    17701 caagaaatca cagtgctaca catttacttc ataaggcatt aagagaagta ttaggagaac
    17761 atgttaatca agctggttct ttagtaactc cagaaagact tagatttgat attactcact
    17821 ttgaagcgat atcaaatgaa gaattaaaag taatagaaga aaaagtaaat aatgttattt
    17881 tatcatcttt agacataaaa tgtgatatta tgaatataaa ggaagctaaa gaaaaaggag
    17941 ctacagcact atttggtgaa aaatatggtg atgaggtaag agtagtttct atgggagatt
    18001 attctacaga actttgtgga ggaactcact tgacaaatac ttctcaagta ggaatgttca
    18061 aaatattatc tgaaggtgga gtagcagcag gtgtaagaag aatagaagca ataacaggaa
    18121 aagctgttta tgaataccta aaagaaagag atggaattat ctctgaagtt tgtgtaaact
    18181 taaaatctaa agaagataat ttaattcaaa gaataagttc tttattagaa gaaaataaaa
    18241 acttatctaa agaattgcat gatatgaaag ctaagatgag tttacaatca gttgattcta
    18301 tttttgattc taaagttgaa gtaaatggag ttaatttgat aactaataaa tttgaaggta
    18361 tggatatgga tacactaaga gaaacagcag ataatctaag agataagctt ggttctggtg
    18421 tagtagtact tgcaaatgta gtagatgata aagttaactt tgtagttact gccactaaag
    18481 atgtattaga taagggaata cattcaggta atattgttag agaagttgca aaaatagctg
    18541 gaggaaaagg tggaggaaga cctaatatgg cacaagcagg agctagtgat gtatctaagg
    18601 ttgaccaagc tttaagctat gcaagtgagg ttattaagac acaagttaaa taacattcac
    18661 tcaaggaggt ttttagtttg gaaaaagatt tagactacac tatgaaattt gaaggaatac
    18721 cagaagatag gatgagtgta ggagatacta ttgattttgt atataaagca ttagttgaaa
    18781 aaggatataa ccctataaat caaattatag ggtatctcct ttctggtgat tctagctata
    18841 taactagtca taaaaatgca agagctataa ttaaaaaatt tgaaagagat gagatacttg
    18901 aagaagtaat cacgcactat ctgaatagaa agtaggtcaa ataaatgaga aaaaagatta
    18961 tgtcgttgtt tgcaatagtc attatgattt ttgtatcaac tgctagctat ggttttgctg
    19021 aagaaaaagg caaggtcata tttattgata tgaataggac caatcttgga agtatgatga
    19081 atattccaat cttgaaaaat gagatagaaa agagaggcta tgtagctctc atgaatataa
    19141 gaggggatca aggtactgat gatagaaggt cttatgcaag tatgggtgca ggaacaagag
    19201 ctagtgtaac tactgaagac tatatcaatt ttcaaaaagt agaccaagat actgctaaag
    19261 cttttgaagt cgcaacaggt caaaaagcta aagaaataaa tgatttaggc attaatagat
    19321 ctataaatga aaatcttgag tatggagaat atggagcaaa tttagggtca ttaggaaaaa
    19381 ctttatcaga aaatggttta aagacatctg taattggaaa tgctgatata attgaaaata
    19441 atcaattagt taaaaacaga aacttatgtt tagtagctat ggaccaatat ggaaggattg
    19501 attcaggtaa tattgataat attaataaga aagattctaa aatgccattt ggaatatcta
    19561 cagattatga taaattacta gatgaaacta aaaaatccta taaaaatagt gatgctatat
    19621 ttgtagaact tggagataca tatagattag atatgtatag attaaatcta aatgataata
    19681 cttatgatat aatgaagaaa aatattgtaa aaaatataaa taaatatctt gaacaagtat
    19741 tttctatggt tggagaaaat gacactgttt atatagcaag tgcatttcca agtgacctag
    19801 attataagaa taagagaaga ctttctccaa taataaaatt tagtggagaa gggaaaggtc
    19861 tactggaatc tgccacaaca agaaaggctg gaattgttgc caatttagat gttggagtag
    19921 atatactagg tacatttggt cttgaaaata aagagatggt aggtagaagt tatactttag
    19981 taaataaaga ggacaatatt agctatctaa aatcacttta tgaaaggata gtgtctatat
    20041 cttcaataag gtcaactatt ataaatggct ttgtaggcgt aatatctgct tcttgggtaa
    20101 ttgccatgtt tgcgattttc tttagaaatt atatgccaca taaagagcgt gtattcaaag
    20161 tacttaagga gtttataaaa ttaggcataa taatgccact atcatttatg ttagctcctt
    20221 tgatgaattt taaaactcca atttcaatat ctttgggaat atttgcaact acgctaatat
    20281 tatacttgtt aggtagaaaa ttatttaagg acaatgatat aaagcacatg ggattttttg
    20341 caacacttac tattttagtc atagttatag attctgtatt tggaacatac ttaatgcaaa
    20401 ataacataat gagttatgat gctgtcatag gagctaggta ctatggtgta ggaaatgaat
    20461 atgaaggtgt aactatagca agtgcagtat ttggcttagc agtattatta cattataaga
    20521 aggtatctaa attacttgtt gtaatttttt cattaataat acttataact agtgcatatc
    20581 cttcaatggg tgcaaatgtt ggtggagcta tatcagaatg tgtagcatat ttgttgttta
    20641 taatgcttat atatgacatt aaaatagatt ttaagaaagc tgtattatta gctgtttcag
    20701 ctgtagttgt cgtttttgca tttgcagctt tagatatact ttcaggtagt gaatctcatt
    20761 taggtatgtt tgtacagaga attttcctaa atggaccagg agagatatta caaacatttg
    20821 gaagaaaaat tcaaatgaat atgcaactag cacagacaag tgtatgggta aatattttac
    20881 ttgtaggaat tggagtaatt gcagtgctta tatttaaacc atctagacac tttagaaaga
    20941 taatgaataa ttttccaatc ttgtttaaag ggtttattgc atctatggtt ggttgtataa
    21001 ttacactttt agttaatgat tctggaatag ttgctgcatc tacagcatct atatatatac
    21061 ttataccact aataataata agtataaata tgataatatt tgaaaaaaat aactaaaaaa
    21121 atttttactg ttcatatagg atttaaataa aaaatgcaat atgattatta atgtaaactg
    21181 gatgcttaca taaaaaatca tattgcaatt tttatgattg attatgtaaa attatatgaa
    21241 gcagtaagcc tgtgacctta gttatgagag gttcagaata tgtatttaat ctatagaata
    21301 taaatagata aaggaggaac tatgttagat ggaagaataa tgggtcttga tgttggtgat
    21361 aaaactattg gagttgctgt gagtgatttg atgggattaa ctgcacaagg tgtaaaaaca
    21421 ataaaaagag ttggcaagaa aaaagatatt gaagagctaa aagcaataat aaaagaaaaa
    21481 caagtaaata aaattgtatc aggattacca aaaaatatga atggaacttt aggtccacaa
    21541 ggagaaaaag taatcaagtt ttgtgaattg gtaaaagcag aaacaggtat tgatgtagag
    21601 ttttgggatg agaggctttc tacagtagcg gcagagaggt ctttattaga agcagatgtt
    21661 agtagacaaa aaagaaaaaa ggttatagat atgttggcag cagtaataat tttgcaagga
    21721 tatttagatt ttaaaataaa ttcataaaag aactataaaa ttgaatactt atgatatata
    21781 atataaataa taaaaataaa aataaatttg aggtgaatga cattatggaa gaaaatataa
    21841 taaatttaat agacgaaaat ggcgttgaaa gccaatttga aataatatta acattagaag
    21901 cagaaggaaa agagtatgca atattaatgc ctttagatga tgaagaagct gaagaagctt
    21961 taatctttag aattgatgag gatgaagaag gtgaaatctt aatacctttg gaaagtgatg
    22021 aagagtatga aactgttgta gctgtgtata ctgctataat ggaagaagaa ggtttaaatt
    22081 acgacgaaga tgaatcaaat ggacttaact agataagtaa ttaagtttgt atataaaaat
    22141 cagatattta ttatatttgc tttaattaaa cattattaat tgtaaaagca tttatagtat
    22201 atatctgttt ttttgttttt tattaaaaat accaaaaaat ttatgaattt tggtgaataa
    22261 tatatctttt atgggtatat atttatatta tgagaataaa ttaaatttat ttaaatgtct
    22321 tgacaaaaaa tgaattatca attacaatat tatttaataa agattatcaa ttgaaaatag
    22381 taagggtgat gttatggcaa atacaatgga tttattaaaa gacaagttaa aagaaactgg
    22441 ttttaagatt acgccacaaa gaagagcaat agttgaaata ttgcttaaac atgaccattc
    22501 acatctaagt agtgaggaaa tctatgattt agttagggtt gactgtcctg aaataggctt
    22561 agctactgta tatagaacaa tgcagctatt ggatgaaata ggattaatat caaaactaaa
    22621 cttagacgat ggatgtataa gatatgagat tagtctacat aaagaagact gtcataatca
    22681 tcatcatctt atttgtaaaa attgtggaaa gataatggaa gctaaagaag accttttaga
    22741 caatatagaa aaagagatac aatcattgta caaatttaaa atacttgatc atgatgttaa
    22801 attttatgga ctatgtgacg agtgtaatgg agtaagcgat tcagaagaat aaacttctat
    22861 tgcttaatgt cgatggaaga atagtataga acttttatat tctatgctat tcttccattt
    22921 tttattataa accatctata aaatgcaaat atacagtttt caagatgctt atagttttaa
    22981 tattgaaaat ataattaatc ctataagtac aaaaatgaat gatttttcta tgaaattact
    23041 aaatggtcct ccaacagtta aaaaacctaa agaaattttc ttttcgctta gcggataaaa
    23101 aattggtatt ccttgctttg taaataaatc tcctaaaaac atatgacttg cataaccaat
    23161 cgtaccataa aaagataaat tattaatatt agtgattaat tctatttgtt ttagtaaaaa
    23221 atatactatt atagttgcaa atatgctatg agaaaaactt ctgtgcttta accagggaaa
    23281 aattgacaac attagacaaa aaattgtaaa atatatttca acttctatga tgtataagca
    23341 tatagctaat agtataaata ttaatgatag aaaaactttt cttagaaatg tgtgggttag
    23401 ttttgcctca ataattataa ttgcaacaaa tgtaattatt gcgcttaaat aaaaattatc
    23461 atttatattt attgaaataa agaaaataat aatattaaca gcatatataa aaattctata
    23521 tatgtatttg gatacattgt tttttagaat gaaatttgag accatagaat tagatttatc
    23581 caaatcgggt agtacagcaa aagtagctga aactaaaata tcaactaaag atataggaat
    23641 tttaaatatt aaagatgttt ggatagctgt taagattcct attgcacaat gagttttacc
    23701 acgcataaat cctcctaaat tatccgatat taaatcatat aatatttaga ggatatcaaa
    23761 ataaaatagg tttgtcaaaa taatacgtta aatgtatttt aaatatagct taaaatatga
    23821 tacgaattat ggtacaataa gaataactgg tagcgagtgc gtgatttgat attatttatg
    23881 attgtagaac gtgttaaaaa attaaataga cagaaggaga tgatgcaatt gttcaaaaag
    23941 aacaccaaca agataaaggt aatggccttg ggtggactta acgaagttgg aaaaaatatg
    24001 actgttgttg agtataaaga tgaaataatt gttatagatg ctggacttag ttttccagag
    24061 gatgaaatgt taggagtaga tatagttata ccagatataa cttatttagt aaaaaatagg
    24121 gataaaataa agggtatatt tattacacat ggacatgaag accatatagg agctcttcca
    24181 tatatattaa agaaaataaa tgtacctgta tatggggcaa gacttagtat aggtcttata
    24241 caagtaaaac ttaaagaaca taaaatgaat aatgttaagt taaatgttat aggtcctaga
    24301 caagttataa agcttgataa tatggaagta gagtttttga aaaataacca tagtatacca
    24361 gatgcttact ctattgcaat acacacagac caaggtataa tataccacac aggagatttt
    24421 aagattgact tgactcctat tgatggagat gttatggata tgcatagaat ctgtgagttg
    24481 agcaaaaaag gagttctttt aatgctggca gatagtacta atgcagaaaa accaggattt
    24541 actatgtctg aaaagactgt tggggtaggt cttgacgaat tatttgcaaa aggaaatggc
    24601 agaagaatta tagttgctac ctttgcatcc aacatacaca gattacaaca aataataaat
    24661 actgctgaaa agtttaatag aaaagttgct atatcaggtc gctcaatggt caatgtagtt
    24721 ggagttgcaa aagaattagg atacctagat atttctgatg acatgcttat agatttaaat
    24781 gatatatgta agtatgaaga tagtgaatta gtgataataa caactggttc acaaggtgaa
    24841 cctatgtcag ctcttgctag aatggctttt tcagagcaca aaaaagtaga aataaagagt
    24901 ggcgatttag ttataatatc tgctcatcca ataccaggaa atgaaaaatt aatatcaaga
    24961 gtaatcaatt tcttatttga aaaaggagca gaagtagtat acagtgatat agctgatata
    25021 cacgtttctg gacatgcctg tcaagaagaa ttaaaactta tacatgcatt agttagacca
    25081 aagtttttta tgccagctca tggtgaatat agaatgttaa aaagacatgc tgaaatagct
    25141 gaacagcttg gtatggataa agaaaatatt tttgtcatgc aaacaggaga tgttttagaa
    25201 ttagataaaa attctgctaa ggtagcaaac cgtatacaaa caggaaacat attagttgat
    25261 ggtctaggag taggcgatgt aggtaatata gttttaaggg atagaaagca tttatcagaa
    25321 gatggtctta tgatagttgt agttactatt tccaaggatg agggtaaggt cttagcaggt
    25381 ccagatataa tttccagagg attcgtatat gtaagagagt cagaagattt aatggatgga
    25441 gcaaaggata taattaagaa tgttctaaat gaatgtgaag agaaaaatat taaagaatgg
    25501 gcttatttaa agaataatat taaagaaaac ctaaaagaat atttatatca aaaaactaaa
    25561 cgaaatccaa tgatacttcc tatcataatg gaagtataat ataaaaaaac tataaatttg
    25621 aaaattagac ctcttgtaat ttttaaagta atatcaaaaa tccaattgat attatcttaa
    25681 gaattacaag aggtcttttt aatactcatt ttaaaaaaat atatattaca taaaatacca
    25741 attataacaa tcttttatta gtaaaaacta cttatatata aattttactg ataggaggat
    25801 tcttatggat gatgtttcaa gacaaaatgc tataaaagct ttaaaacaaa caaaaatgga
    25861 aatagcagga gagtatggaa tgaattatga agatgctttt gaaataatag aaaatgcgtc
    25921 taataaaggt gttttagaag gttactttaa aaagcttgaa aagaaaaaaa atttaggtca
    25981 aggtatatct aggcatttag aataaaaaaa gttaaatgtg agaaaataag tttaaataaa
    26041 ctttaagagg tgacatcgat gaaaaaatta gctagtactg ctttggctat attaattgcg
    26101 cttactcctt taagcttttc ttttgcaaat aataaggaaa atgctgatgc caaccaacta
    26161 aatatatctt caaaatcagc aattttgatg gatgttggta gtggacaaat tttatatgaa
    26221 aaggatgctc ataaaaagct tcctcctgct agtgtaacta aggtaatgac tatgctactt
    26281 atagtagaag ctttggattc aggcaagatt aagcttgatg atgaagttca agtaagtgaa
    26341 actgcatcta gtatgggtgg tagtcaaata ttcctagagc caggagaaac tcaaaaggtt
    26401 gatacattat taaaaggtat agctgttgct tctgcaaatg atgcttgtgt tgctatggct
    26461 gaacacttag ctggaagtgt tgaaggattt gttgatagaa tgaatgctaa agcaaaagag
    26521 ttaaatatga atgatactca ttttgcaaat acaaatggac ttccagttgc aaaccactat
    26581 acatctgctc atgatatagc tttaatgtct agagaactat taaaacacga aatgataagt
    26641 aaatacttaa ctacatggat ggataaggtt gtagtaggga agaaacaagt tacagtaggt
    26701 cttgcaaata ctaataaact tataaagcat tatcaaggag caactggtgt taaaactggt
    26761 tttactcaag aagcaaaata ctgtttatca gcatctgcaa agagaggtaa tactcatcta
    26821 gttgctgcaa ctttaggagc agagacttct ccagaaagat ttaatgatgc ctctagcctt
    26881 ttaaattatg gatttgctaa ttatgaaagt gttaagttat gtagtaaagg tgataatata
    26941 gcaacactta cattggataa ggctgatgaa aacaaagtta aattggttgc taaagaagat
    27001 ttaaatgctc ttattaaaaa aggaagttct aaagaatttg agaagaaaat tgaaatagta
    27061 aaaaatccta agatgccaat taaaaaggga actgttttag gaaaaataaa gatatgtaaa
    27121 gataaaaaag taataggcga agtggaattg ataaatacta aggatataaa taaggcaagc
    27181 tatctacaaa tgcttcaaag aataattgat aatatgatat aaataattat cagatgaaat
    27241 ataattataa tacacaaaga tatgattata atacacataa aaaataccct tatgtaatta
    27301 tttgccatag gggtattttt atttaccata tagaagtatt ttataatctt atgattttta
    27361 tagaaatata gaaaagttta tgtatcttaa aatatcattt gatttaatat catatatagg
    27421 gttgtttaac aattatctaa gtaaaggcat actaccagta agtttattca ctttcttttt
    27481 attcccataa attgctatcc caaaatactg taactcatct tctggaacac tagcaatctt
    27541 ttgtgtaaat tcttcataaa gattgcaact ttgtgctaca tctgaaaaat ctacaacagt
    27601 taaatcgtta aattccaaag tatataattt tttacgtaaa tcttttatta tttctttgtt
    27661 tccttttaaa ataggtacag gaatgtcaat aattcctaag tggctattac ctgttttgtc
    27721 aattacatct gggccaacag tttctggggc atgttttcct agtgtaattc ccattatagc
    27781 tgctgtattg gatataattc ccattggtaa attttcgtct attaccatga cacattttga
    27841 atttttagtt tccattttta tttctccttt ttaccttatt taaagttgtt tgaatcatca
    27901 tttataaata tattttgata ctgctttggt gtaagaccaa taaaattctt aaaaaagttt
    27961 gtaaagtgac tttggtctac aaatcctgtt tgtatcgcag catctatagg ttctacgcct
    28021 ttttctaaaa gctttttagc tttgtttacc cgtatatttt ctaggtatct atatggtgta
    28081 atacctttta atttcgtaaa attacgaagt agagagtact tattcatacc tgctatagtg
    28141 cttaattcgt caagtactat atgttcagca taattatttt ctagataatc acatactgct
    28201 tgaatttcta tatttgtatt ttccaagtta gattgcaaat ttggctctgt atgttcttca
    28261 attaattgtt caattaggaa gaaaaaaagc tcttcctttt taaaatccaa ttcttcttcc
    28321 atgattatat agtgtaattc ttgtagtaga ggtactagct cactgcgaaa tataacaggt
    28381 tgattaaatt ttggtaaata atttttacct gtaatttcaa agacagtttt tttcattatt
    28441 tctggcttaa tattaataca tctgtaatca agtactttat catctatttg ttcacaagta
    28501 tgactatcaa aagggttaaa aagtagcaaa tctccagtac ttgtggtata ctctttattt
    28561 ttacaagata aatatctttg acctttttct ataaaaccaa ttacataatg ttcatgaaaa
    28621 tggtttggga atttttgcat tatgccttta aatctatatg cttcaatttt taattcccta
    28681 tcaaagcata ctgtgcgttc ttcttgtctc acttaggtaa cctcctttca tgttatacaa
    28741 agtgtaacac atattttttt atacttcttg tataatattg actaagagac aataatgtca
    28801 attcatgtca atataatatt acacttacag taggtgtaat tattatattt tcacaatgaa
    28861 tatgatataa taataaaagt tataaaataa aaagcaaatt ttaattttat gcctagaagt
    28921 taaaattatt ataatgtaaa ataattttga ttataagcta tttaattgtt ataataatat
    28981 taaaaattta atttaggaga tgtacattaa tgttaagtat gaatatagga gaaatagtag
    29041 caagtttagt tggtatagct atggcaatat caatacatga gtttggccat gcttattcag
    29101 ctcacttatt gggagatgat acagctaaag cttatggtag aatgacatta aatcctgtaa
    29161 gacatgttga cccaataggt cttttggcta tgtttatcct taagataggg tgggcaaagc
    29221 cagtaccagt aaatccaaat aactttaaaa actataagat aggaaacctg atagtttcac
    29281 ttgcaggcgt tttttgtaat gttttgactg ctatactgtg tgtaattatc aataagtatg
    29341 tgcatatgta tgctataaat acaatagctg aatatgtgtt tttatatagt attggatttg
    29401 cagcttttaa cttacttccg atacctccac ttgatggatg gggagttata tctacatttg
    29461 taccatataa atggaatgaa tatttgtata aatttgaaag catgagttat ataattttat
    29521 taattgcttt atttactggt atgtatagaa taatattaac accaatatat gcagtgttgc
    29581 taaaaatagt atatttattt gtttaatata ataaacagat taaagaggga ttgatatgaa
    29641 gtataatata caattacaag tctatgaggg accattagat ttactttatg atttaataac
    29701 aaagcataaa atagatataa aggatatatc cataatagat ataactaagc agtatctaaa
    29761 ttatttgaaa atgctagata aaatggattt agagataact agtgaattta taactatggc
    29821 atcaaaactt ttagagataa agtctaaata tctgctttat aagcaaaaag atgaagaaga
    29881 agacccaaga atagaactta tggaaaagtt agaagaatat agaaagttta aggtagcatc
    29941 acaagatata aaagaaaata taacttatgt taatgagaga ttttatagaa ataaagaaga
    30001 gataattatt gatgacaatg tggatttaga agatatatca atagaagcaa taaaaaatat
    30061 acttccatat atatttaaag ttaaaacatc acagatagaa aatgctaatg atgagaaact
    30121 agataaaata gtaaggaaga aaataatatc tgtagaagaa aaaatattat atataagaga
    30181 tattataaaa gagaagattg aagttacttt tacaaatatt ataaaatcat atgaaaatga
    30241 tgaaataata gctacttttt tatctatatt agagcttata aaagaaaaag aaatagtcgt
    30301 agtacaagat atattttttg atgatattct aataagaaaa agttcggagt gttaaaatga
    30361 aaagagaaga tataaagtat attatagaat ctgttatgtt tgcatatggt gaaccaataa
    30421 gtataaaaga attaaactat ataataaata aagaactttc atcaaaagaa atagagatta
    30481 tgttaaattt acttattgag gagtacagag aacaaaatag aggtattcaa ataatcaagt
    30541 tagagaataa gtaccaaatg tgtacaaata aagactatgc agaatatata aagaagatta
    30601 ttgaaccaaa gaaaaagaaa agtttgagcc aagctacatt agaaactctt actataattg
    30661 cctacaaaca accaataaca aaggtagaaa tagaggatat aagaggagta aaatgtgata
    30721 aggtattaca aactttgttt gaaaatgaac ttataagaga agctggtaga ttgaataaga
    30781 tagggaaacc aataatttat aaaacaacag atgaattttt aaagttgttg aatatagaaa
    30841 gtcttgaaga attgccacct atagaaaatt atcaagaagt ggcaacaaat gaataaaaat
    30901 ataaaatcac aataatttag tatgtaattt taaattttaa aagatttaaa atgctctaag
    30961 ttgttgtgtt tttttgtata aatataaaat tataattctc ttaaaataaa tatggat
//
LOCUS       DAYAOY010000048        27712 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYAOY010000048 DAYAOY010000000
VERSION     DAYAOY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.048"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(443..1252)
                     /locus_tag="ACGI6B_002188"
     CDS             complement(443..1252)
                     /locus_tag="ACGI6B_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9751630.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1336..1869)
                     /locus_tag="ACGI6B_002189"
     CDS             complement(1336..1869)
                     /locus_tag="ACGI6B_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9751631.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(2019..3368)
                     /locus_tag="ACGI6B_002190"
     CDS             complement(2019..3368)
                     /locus_tag="ACGI6B_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HFG9751632.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3380..4762)
                     /locus_tag="ACGI6B_002191"
     CDS             complement(3380..4762)
                     /locus_tag="ACGI6B_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HFG9751633.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            5006..6220
                     /locus_tag="ACGI6B_002192"
     CDS             5006..6220
                     /locus_tag="ACGI6B_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9751634.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6397..8310
                     /locus_tag="ACGI6B_002193"
     CDS             6397..8310
                     /locus_tag="ACGI6B_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751635.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8581..9555
                     /locus_tag="ACGI6B_002194"
     CDS             8581..9555
                     /locus_tag="ACGI6B_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFG9751636.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9576..9851
                     /locus_tag="ACGI6B_002195"
     CDS             9576..9851
                     /locus_tag="ACGI6B_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9751637.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9864..11027
                     /locus_tag="ACGI6B_002196"
     CDS             9864..11027
                     /locus_tag="ACGI6B_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9751638.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11364..13643
                     /locus_tag="ACGI6B_002197"
     CDS             11364..13643
                     /locus_tag="ACGI6B_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9751639.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(13738..15078)
                     /locus_tag="ACGI6B_002198"
     CDS             complement(13738..15078)
                     /locus_tag="ACGI6B_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9751640.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(15090..15872)
                     /locus_tag="ACGI6B_002199"
     CDS             complement(15090..15872)
                     /locus_tag="ACGI6B_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751641.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15887..16909)
                     /locus_tag="ACGI6B_002200"
     CDS             complement(15887..16909)
                     /locus_tag="ACGI6B_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9751642.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16915..17928)
                     /locus_tag="ACGI6B_002201"
     CDS             complement(16915..17928)
                     /locus_tag="ACGI6B_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9751643.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(18056..18994)
                     /locus_tag="ACGI6B_002202"
     CDS             complement(18056..18994)
                     /locus_tag="ACGI6B_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9751644.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            19264..19728
                     /locus_tag="ACGI6B_002203"
     CDS             19264..19728
                     /locus_tag="ACGI6B_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751645.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19840..20157)
                     /locus_tag="ACGI6B_002204"
     CDS             complement(19840..20157)
                     /locus_tag="ACGI6B_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9751646.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(20170..20901)
                     /gene="chbG"
                     /locus_tag="ACGI6B_002205"
     CDS             complement(20170..20901)
                     /gene="chbG"
                     /locus_tag="ACGI6B_002205"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFG9751647.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20927..22237)
                     /locus_tag="ACGI6B_002206"
     CDS             complement(20927..22237)
                     /locus_tag="ACGI6B_002206"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFG9751648.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22334..23647)
                     /locus_tag="ACGI6B_002207"
     CDS             complement(22334..23647)
                     /locus_tag="ACGI6B_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9751649.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23669..23980)
                     /locus_tag="ACGI6B_002208"
     CDS             complement(23669..23980)
                     /locus_tag="ACGI6B_002208"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9751650.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(24180..24884)
                     /locus_tag="ACGI6B_002209"
     CDS             complement(24180..24884)
                     /locus_tag="ACGI6B_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9751651.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24975..26336)
                     /locus_tag="ACGI6B_002210"
     CDS             complement(24975..26336)
                     /locus_tag="ACGI6B_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9751652.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26835..>27712
                     /locus_tag="ACGI6B_002211"
     CDS             26835..>27712
                     /locus_tag="ACGI6B_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9751653.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKII"
BASE COUNT         9775 a         4550 c         3530 g         9857 t
ORIGIN      
        1 gttgatacaa ataaaatatt atgttttttc atagtcccac ctcctgttta acgagtatag
       61 aaagtaataa tttggtctat tgtagcactt tttaaaaaag ttttaaatct taaaaatcaa
      121 attttgtacc atttactatt attttccata ataaaataat gtaagctatc ttttttcaca
      181 tataatttaa tttctaaaac ggttagtttg acattattgg aatataaata aaaatttaat
      241 ataaattcgt taatatatta atacattttt aagaatgaca aggaataaat tataacaaat
      301 tttaggaata tataataata gggtctcaga acttagttac aagttctaaa accctatttg
      361 tttagtgtaa aaaacatcat aaaatttagc ttaaatcttt tcacataata ttactctttt
      421 tctaagtgat aataaaatat atctattcct ctaaaaactg taaaaattcc tgattaaata
      481 gtttaagctc atcatagaat atagcatgac cactatactc aaaagtatat aaaattgaat
      541 cttctatttt ttcattcata aaaactgcaa attcatacgg acatatttca tctaatttcc
      601 catgaaatat accagtaggc actcttacac attttaaatc attaaataat tcttcatctc
      661 ttaaagatac agcagttcca atagtgccaa tacctgatgc actaaaacca atatcattaa
      721 accatcttct aaagctttct ggtggatttg atgcaaatac tttttcgcca aaatcggtta
      781 ccatttctgg tctatcagta catgcttgtg ctatcaattt atttacatct tcacgtgtca
      841 taccatatgg aaattctgga ggtctttgta caaatgatgg tgcagcagct gcagctaata
      901 ccaattttga cactccatat ccattaaata aactcatata cctaagtacg attgctcctc
      961 ccattgaaaa accaacaagg gtaaagtctt ttaatccaat agcatgtacc accttataaa
     1021 tatcgtctgc taattggcta taagtatatc ctcctgatgt tgcatcagat ttaccaaaac
     1081 ctcttaaatc aattgaaact gttctatatc cgagttttgg caatatattt gtttgatatt
     1141 caaatatctt atgtcctaag ggccaaccat gtataaaaag tacagttttt ttagcactcg
     1201 gattcaaatc atatactgct atgtttatat cattcacata tacataatac atatcaacag
     1261 caactccctt caagaaaatg tatgatggtt attttatata tatgaataaa atgatgaata
     1321 actaaatttt caaatttatt tacaatactt ctctattgcc ttttcaaata aacttacatg
     1381 aagtttttca tctaggatta ttctatttaa tatagctttt atataagggt cttcaataag
     1441 acttatatct ttataataat tattaatagc cacatgctcc aaatgcaaat ctagttttaa
     1501 tctgttacaa atagaatttc cataacatac aaaaccacca ttccaatatg caccacaaga
     1561 acttgtagaa cctctatata caggatttcc acccaataat cttatagtct ttgctaatat
     1621 ctccatatgt aacatttctg ttatagatat atttatccac atttcactta actctctatg
     1681 cgtttcatct aaatcaaaat tatggtatag atattgattt acactcgtaa attcacttga
     1741 tactccagca tagtcatcca tcaataattc tgcataatac ttatttggac ctaaaacttt
     1801 tatctctgga tatggctcat cacttgaata tccatttcgc ttgtgcatgt cataatgtgc
     1861 accactcata atatctcctc ctttaaattt aatgtatgat attaattgta tgcatcacaa
     1921 ctttttcata taacttagat aataaaatct acttaaataa taaaaagcca tattaattaa
     1981 aattaacatg accttttatc cacaaaatta ctttttaatt attcatcttt tccagctact
     2041 gatagctctt taataacaac tgaaggtgaa cctatactac tcattggaaa ttctaaatct
     2101 tctcctatag cttcaatatc tttcaacagg tcaaaatagt ttcctgctac tgtaatttgc
     2161 tccactggaa atactttttt tccttctttg atataaaagc ccttggcagc taaagaaaag
     2221 tctcctgtta ctgaatttgc acctgaatga agtcctgcaa agctagtaac catcaatcct
     2281 tcatctatat cctttataat ttcatctaaa gatttatctc ctttctctat ataaaaatta
     2341 gttggatata tgcttatagg tgatgaatat gaagatttta ttccatttcc tgtagtcttt
     2401 acacctgcct tattagccgt ttttaagtta tgaagtaaag ttataagctt tccatttaaa
     2461 acaacttctt ttttaaatgt agctactcct tcatcatcaa aaggagtaga tgccatacca
     2521 ttttctaaaa gtgggtcatc aactattgta actataggag atgctatcat gtctccttct
     2581 ctatttttca ataaagataa tcctttttga gctgaatctg cattaaatat cccagaaaat
     2641 gtgctaagca aagataccat agcttcatta agcaatatag ttttatattt acctgatgta
     2701 atactcttac cacctacttt agataaagca ttatcaacac caagttttgc tatctcacaa
     2761 ggttttattt cttcaataga atttgctata ttatatccta ttccatcata tttttgtcca
     2821 ttgtcttcta tgataggaac tacaaaccct ataagcatat tagttttatt gcttaaatta
     2881 agacctttag tattagatat accattactt gatacagaat aagatatagt acatttagat
     2941 aaatttacca ccttatctga ataagcttta gtctctcttt ccatttctaa agctaaatca
     3001 atcaacttat ctgcctctaa attttctagc tctttagaat aagtctttac atcattatag
     3061 tgcttatctc cttcatatat aaattgtaca tcttcatttt caatagtgtt tactccatct
     3121 tttacatttt ttatcaacat atcaacagct ttatcatcta aaatttctgt ataagaataa
     3181 cctatctttc catttacctt acctctaaaa gataaaccaa aagatttatc aagattgtat
     3241 ttctcaactt ctccttcata tatatttata cttaaatttt ctcctgtata ataatatatt
     3301 tcacactctt caaaaccttc acttagagcc tttttaaata atatatcttt aaaattttta
     3361 agttccacaa ttatccctcc tatcttccac ctacagtaat ttcttttacc cttatcatag
     3421 gttggcctac attagttggt atacttcctg atgatgagcc acacatcccc tgagcttgtt
     3481 ttacgttatt cccaaccata tctatattca taagtatatc gctacctttt ccaattaaac
     3541 tagcacctct aactggctct tgtatttctc cattttttat taagtaacct tctgaaactg
     3601 caaaattaaa ctctcctgtt acaggattta cagaaccacc acccattttt ttagcataaa
     3661 gtccatctgg tattgacttt ataatatctt ctggtctatc ttctccagca gcaatataag
     3721 tgttagtcat ccttgaagtt ggtgcaaatt tgtagttttg tctacgagaa cttcctgtag
     3781 gattcattcc cattcgtcta ccattaagtt tatctatcat ataagacttc aaaattccac
     3841 tttcaatcag tatattcttt tgagttggat ttccctcatc atctatattc atagaacccc
     3901 agtagtttgg tattgtacca tcatctatag ctgtaatctt tgtggaagca atctgttgac
     3961 ctaatttatc tgcaaataca gagttcccct ttgcaactga tgtagcttcc aaagaatgtc
     4021 cacaagcttc atgaaatatt actccaccaa atccattatc tattgctacc atcatattac
     4081 ctgcaggaca atttttagca tgcaacatag tatgcgctac tctagctgat tcttttgcat
     4141 aatattctgg gtctatttct tcaaatattt caaacccttt acaacctcca ggaccttcaa
     4201 atccagtttg attttcatta cccttagatg caattgaact tatccctagt ctagttctta
     4261 ttcttctatc ctcaacatat ataccttcag tgtttgcaat taatattttt tggtctttat
     4321 ccaaataact gacacttact tgagatatat cattactaaa ttcttttgca ctcttatatg
     4381 caaccttcat aacacttact ttatctttat ttcctattga atttggataa atttttatat
     4441 tatgtatgtt atttatttta atactgtcat ttaatattac actttttcca tcttccaatt
     4501 tacctaaagc aacagcagct ttgtatgcta catccaacag actatttaat gaattgtcac
     4561 ttgtataagc atatatactt ttcaatcctt taaatattct tattcctata ccataatttc
     4621 ttccacctat tgcattttct actttattat ctattaaact aatagaatta tttatagtgt
     4681 cttcttcaaa aatctcagca aaatctcctc cagtaattag acatttttct aataccttag
     4741 aagctatatt ttttgaaagc ataaatttac ctcctacttt aaatttttaa ttaactttta
     4801 ttcaatcttt ttacttgtta acaaacatta tatactatat tacttaaaat aaggtaatta
     4861 tgaatatatt atacatatgt tttatactaa aatgttatca cattttatct atacataccc
     4921 atataactag ataattaaaa tgtataatat tatttgctat agaaaataat acactcatac
     4981 ataataaatt ttaggagtga agtatatgaa tcaagaatat gtaaaaggaa atcctatttt
     5041 tactaatatt aataaagttc caagacaatt tccatacctt actgatgata ttgatactga
     5101 tgttataatt gttggtggtg gcgtaactgg atgtatttgt gcttactatc tagctaaaaa
     5161 caatataaag tctgtcattc ttgaaaaagg aaggattgca catggaagca ctagcgtgac
     5221 aacttctcta ttacagtatg aattagatga caatttgata gatttaacag aggttatgac
     5281 tctaaaagat gctctaaaag catacaacct ttgtacttca gcacttgaag aattagatac
     5341 atttatagag ctatatggaa ataagtgtga ttatgccaaa agagatactc tactttatac
     5401 tgccaacaaa cttgaagtta aggctatcaa agaagaatat aacttaagaa aagaaaatgg
     5461 atttgatgtt gaatatattg atgaatccac aaaccctttt agctttgatt taaaatcagg
     5521 attgatagca aagaatggag gacgagaatt agacccatat aaatatagtc accatttgat
     5581 tgatgtaagt ttaaaaaatg gtcttcaagt atatgaaaat acagaagtaa aaaaagtaga
     5641 tttatcaaat gataaggtta ctgctgaagt atcgtatgga tacaaagttc atggtaaaaa
     5701 attaattgta gctacagggt acaatacaag tttatttaca aaaagaaatt ttgcaactaa
     5761 gtcaaatact tttaatatag caacgaaacc acttaaaaac atagcttctt ggaaaaacaa
     5821 cattttaatt agagataact gtgacccata taattatcta agaactacta aagacaatcg
     5881 attaattata ggtggtgaag atgtaagttt tgatgatata gaaaatgaaa ctttagccaa
     5941 tgaaaagtat gatattttag aacaaagatt gaaaagtatg tttaaggata taaaagacat
     6001 tgaagttgaa tataaatatt gtggttgttt tgcttcaact ttagataatt taggttttat
     6061 tggtccagat aataaaaata acaatctatg gtattgctta ggttatggag caaatggtat
     6121 actctttgca atattaggag gtataatgtt aagtgaatta tatcttggca aacaaaataa
     6181 aaatatgaaa ttatttaaag tagatagatt tgataaatag atattatata ttaaaagact
     6241 gtaaaaagat gaaactttat aattctaaaa gttaactttg atacagtctt ttataatgtt
     6301 ccttttaaaa atactttatt tataattaaa tataaagtaa aatgttaaaa tataactaat
     6361 aaaatttagt cataagataa tatttaagtt tatttgatga aatatggagg aattattatg
     6421 aaggaaatac tcgttattgc tcccactaaa ggaacctatg aaaaatcaat tcacatagtt
     6481 aaaaaaaaca aatatacaaa tattgatgtt gtatttggca atctaaaaga aggtatacct
     6541 cttgctgaaa aaagtattaa tcatggtact agaattatca taagccgagg tggaacttac
     6601 aatatgttaa aagctactta caatatacct atagttgaaa ttaaagtaga tgcttatgat
     6661 attataaaaa gctacaagga ggtaaaaaat tccaacgaac catttggaat aattggattt
     6721 aataatgtca tctacggttt cgatattata gaagaaatat tgaataagaa aataactatg
     6781 attgaaattg aaaaagaaga agaaatttat gatgctatag aaaagtacag aaagaaaggt
     6841 ataaacacat atataggaga taccactgtt gcacatattg ttaagagact taattgtaag
     6901 ggaatattaa tcgaatctag agaagaaaac atacttagag ccattcaaca agcagaacaa
     6961 atactcgaag caaccaagga tgaacaaaaa agaagattac agatagaagc tatgacagat
     7021 tttgtacatg atggaataat aacagttgat aaaaatttta aaataacatt atttaataag
     7081 agtgctgaaa aaatatttgg tataaaaaaa gaaaatgcac tttatcataa tgttgtagat
     7141 gttatcccaa acacagttct tccacaagta gttgagacta tggaacctca aattggtgaa
     7201 atacaaaata tagggagtac aaaaattatt acaaatagag ttccaataat tgttgatggt
     7261 cagattcaag gggctgtttc aacatttcaa gatagcaagg agataagttc ttttgaacat
     7321 acaataagaa ggagcttatt aagcaaaggt ctatctgcta aatacacttt tgatgatata
     7381 attcatgcta gtgaaaagac aaaagattgt atagaacttg caaaaaaata tgcttgttat
     7441 gatactccta tgattattac tggagaatct ggagttggta aagaactatt ttgtcaaagt
     7501 atacataact atagtaagcg taaaaatgca ccttttgttg ctgtaaattg tgctgccata
     7561 cctccatctc taatagagag tgagtttttt ggttatgagg aaggttcatt tacaggtgct
     7621 agaaaaaaag gtaaagcagg tgtattcgaa ctagcacatg aaggcacaat cttcctagat
     7681 gaaataagtg aaatccctct tgaacttcaa ggtcgattat tgagggtatt gcaggaaaaa
     7741 caagttatga gaattggtgg tgataaagtt attcctatag atgtaaaaat tatctgtgct
     7801 tcaaataaag acttaaaaaa tatgatgcgc aatggtttat ttagacaaga cctttatttt
     7861 agaataaata tactaacatt atatcttcct tcattaaaag aaagaaaaga agatattata
     7921 aaattatcag aattttttat acataaatat tcaagcaaat acaataaaac gcctttaatt
     7981 atcactccaa atattaaaaa ggcacttctt gaacgagaat atgaaggtaa tataagggaa
     8041 ttggaaggta tgatggaaag ggctgttatt gtggaatcat ttgactcgat tttattagaa
     8101 aagcttagca atgaatatac aaataatata gttgataaag ataatagtga ttttgaatgc
     8161 agtaatttag atttaaaaac tttagaaaaa aaatatatct ataaaattta taacgaaaat
     8221 aatagaaata ctacaaaaac ctgtgaaatt ctaaagataa ctcgctccac tctatggcgt
     8281 aagttaaaag aaatagaaga tgatgtttaa aaataataca tatctatata tatcaaaacg
     8341 ttttatgttt aattttaata cataagacgt ttatttgttt tcaataaaaa atacaattat
     8401 atgctaaaaa gaatctttgt aagtgaaatt ctcgtcaatc atacttttaa ttataagtaa
     8461 tctacagcat ttcaatcgat tattaaaaat aatcatgata actaaagata caaactctat
     8521 atattggcat agtaattgct tttataatac ctatataaaa atgaaaggga gtgatactag
     8581 atgcacatct taaaatcagt gaaaaagata ccaggaggac ttatggtctg tcccttgtta
     8641 ttgggagcac tatttaatac agtatgtcca caagctcttg aaattggagg attcacaaca
     8701 tccttattta aaactggtgc aatgagtata cttgctctat tttgtctatg caatggagct
     8761 caaatcaata tcagacaagc tggtaaacca ttgactaaag gtatagtact tacagcaaca
     8821 aaatttataa ttggtgcagt attaggtatt attgttagta aatttattgg acctaaaggt
     8881 ataattggta ttacacctct tgccatagtt gctaccatga ctaattcaaa tggaggtcta
     8941 tttgctgctc ttgcaggtga atatggagac tccacagatg ttggagcaat atctatctta
     9001 tctctaaatg atggtccttt ttttacaatg cttgcttttg gagtaactgg acttgcaaat
     9061 gtacctatag tagcactatt tgcagcactt ataccaatac tacttggatt tatacttggt
     9121 aaccttgatg aagatatacg agaatttctt gcacctggta caacattatt aattcctttc
     9181 ttcgcttttc ctttaggagc agcattaaac tttaatcaaa ttataactgc tggactacct
     9241 ggagttgtac ttggtttagg agttactata ataacaggta tgggtgggta ctttgtaatg
     9301 aaacttatac gtgcagaaca tccagcagta ggtgcagcta caggtacaac agccggaaat
     9361 gcagctggta ctcctgaagc tttagcagct atagacccta ctctagctac aatagcagct
     9421 gtttcaacag tacaagttgc tgctgctatt attgttacag caatatgttg tccaatgtta
     9481 gtttcctatc tagataaaag agaaaaaaag aaaaaatcta tttttaaaac aaataaaaat
     9541 attgaagtaa attaatactt aaggaggtaa aattaatgaa tgcaattatt catgcaaatg
     9601 aaaaagataa tctagttaca tgtgtcagac ctttaattaa aggtgaaaaa gtttcaattg
     9661 atggaaagtc ttacatagta aatgataata tacctgtctt tcataagatg gctacagaag
     9721 atgtaaaaaa aggtgatgtt gtatacaaat acggtgaaat tataggaatt gcaacagttg
     9781 acataaaaac tggtgactat gttcatgtac ataacattga aagtacacgt ggtcgtggag
     9841 ataaaaaata ggaggaagtt aaaatgaaat taatgggtta tttaagagaa aatggtcagt
     9901 ttggtattcg taatcatgtt ttaattattc caacatctgt atgttcaagt gagacagcta
     9961 caagaatagc atcacttgtg cctggagcaa tagcaatccc tcatcaacat ggatgttgtc
    10021 aaattggttc tgatatagag cttacagcaa agacattaat aggatttgga aaaaatccta
    10081 atgttgccgc agttttagta gttggtcttg gatgtgatgg tattcaagca aaagaattag
    10141 catctgaaat agctacaaca ggaaaaaagg taaattacgt tgttatacaa gaatgtggtg
    10201 gaacattaaa aacagtttct aagggagctg aaatagtaag taaaatggct cgtgaagtat
    10261 caaaagaagt aagagttgaa tttggtatga gtgaaattac attagcactt gaatgtggtg
    10321 gttctgaccc aacaagtgga atagcttcaa acccttcaat aggcgttgca tcaaaccttc
    10381 ttgtagatga aggtggaagt agcatactaa gtgaaactac agaagtaatt ggtgcagaac
    10441 atttgttggc tacccgtttt gaagatgaag aaatgaaaga taagtttctt aaatttgtaa
    10501 gtgatgtaga aaaaagagca attgctatgg gagaagattt acgtagtggt caacctactc
    10561 caggcaataa agctggtgga ctttcaacaa ttgaagaaaa gtcacttggg tgtatgtata
    10621 aggcaggcaa taagccattc aaaggtgctc ttgaatatgc tgatattgta cctcctgata
    10681 aaaaaggatt atattttatg gatactccag gtcaagatat agattctatc actggtatgg
    10741 tagcaggtgg tgcacaaatt gtaatctttt caactggtag aggtactcca acaggaagcc
    10801 ctatttcacc agttattaaa atcactggga atagtgatac atacaacaaa atgccagaca
    10861 atatcgatat aaatgcaggt agaattataa cagatggtgc taaaatagca gatataggtc
    10921 aagaaatatt taatgaaata atagaagtct gtaatggtaa acataccaaa gctgaaagtc
    10981 ttggtcatag agaatttggc atttacagaa tttcatctac attttagatt gtaacaaaat
    11041 atcttttata aatataatac ttatgtagat acataagcta aaagttcttt ctaatactat
    11101 aaaaattttc tatactgaaa aggaactgta tcaaaattat tttgaaaaca gttccttttt
    11161 taacctcttt tactcaaaat attataaaat aagatttaaa caataaacgt atcttgatta
    11221 atctctaaga agacataaat aaagctaaat gtaaagtggg ataaataaaa tttaatatct
    11281 ttgtatgaaa agatatttgg tataatatgt ctgagagaat tcttatttgt aaacaaataa
    11341 aggatggagg atgaaacaaa agaatgaact ttgaaaacac aaaaaataaa aaaataatag
    11401 ctactttact attgacactc cttttaatcg tgtcattaac atgtgtcact tttattatga
    11461 atctaaataa agtaacacat aatgaaacag aacgatatct tgtagaagtt tcacaaagta
    11521 tttccactac aattaatgtg aaatgtaaaa gtatatttag gacactatat tctatgggag
    11581 atacttattt tgagattaat gatagtgaaa aagaagtcaa tagatactta tcaaaaaaat
    11641 taaaattatt tgaatttgat tggttaggta ttatagattc taatggaaat tttgtaagtg
    11701 cccttaaaga tacaggtaat gttaaaggac aagactttta tagaaaagcc cttcaaggca
    11761 aagaaacaat tactcctgtt aaatcacaat ctggtacata tggaatttta tattctgtac
    11821 cactatacaa tggtgataaa attgttggag ctgttgctgc ttggaatact attgatacta
    11881 tgagaaattt attaagtgta gaaagctttc atggtgaagg attttcccaa attatcgacc
    11941 aagatggaaa ttttattgta agttcagcaa acaaaaatgc tccaaaaaat gtggtaaatt
    12001 tttttgacat gattaaacac caaggaaaac aggttgataa aaataaactt gaagaaatga
    12061 aaataaactt aaaaaagaac aaaactggtt ctatgtatta tacattaaat gatggtattt
    12121 ctaaggcaat gcattatctt ccattacaag aagaaaactt atacctcctt tcaattgttc
    12181 caacaaaaac agcaagtgca cagtttgatt tacttctaaa aaaagcaatc ttcatcaata
    12241 ttttaattgt tatactattt ttattgctta ttatattgat tatgtttata aatcaaaaga
    12301 acagggcaca acttataaaa atcgcctatg tagactctgt aactaaaggt tacagtaaag
    12361 cacgttttga tattgaagct aaaaaaatta tacagtcatc ttcatcagaa agctattcat
    12421 tggtatctat aaatattcag aaattcaaac ttattaatga ttcttttggt agtgaagcag
    12481 gagataaaac attaaagtac atacatgatg tcattagtaa ttatctagaa ccaggagaat
    12541 tgctttgtcg agtttctaat gaccagttta atgtacttat taaaactatt cctcaaaaag
    12601 aaattcttaa gtacatagaa aatatcacca atgatattaa tagtttcaat aaaaatgaag
    12661 aatgtaaata ttttatagct ctatctatag gtgtatataa aattgatgac ccaaatctac
    12721 ctttagtttc aattcaagac cgtgcaaatg ttgcacgaaa aaataataaa ggaaacattg
    12781 gaaatcatcc actttataca tgtgtttttt atagtgattt agagcgttta aaaatgatta
    12841 aagaaaaaga aatggaaaat agaatggagg aagcattaaa taataacgaa tttatagtat
    12901 atcttcagcc taaaataagc ctcaaagata atactattgt aggtgcagaa gcattagtaa
    12961 gatggcaaga cccaaataaa ggtcttatac caccagacga gtttattcct ttctttgaaa
    13021 aaaatggttt tattacaaaa ctcgaccact atgtttttga aaaagtatgc agtatgataa
    13081 gaaaatggat agataatgga gaaactccaa tacctgtttc agttaatttt tcacgtgcta
    13141 atttaaataa tatagatttt ttagaaaaat acagaatgct tcgtaataaa tacaacattc
    13201 ctgctaagct cttagaaatt gaattaactg aaacacttgt atttgaaaat ctccaaaaat
    13261 taattaatat aatagaccaa atccatgaag aaggttttca atgttcacta gatgactttg
    13321 gaagtggata ctcttcactc aatatgttaa aagaaataaa agttgacaca ttaaaacttg
    13381 accgtgcttt ttttagctca cctaatgcag ataatattgc tgaaaatcat gtaatagaat
    13441 ctatagttaa tttggcaaaa aaattaaata tgaatagtat atcagaaggt gttgaaacaa
    13501 tcttgcaaat ggattatctt aaaaaaatta attgtgatat gatacaagga tatgtttttt
    13561 caaaacctct acctcaagaa atatttgaga aaatgacttt tggaaaaaaa ctagaagaaa
    13621 ccagtatgga tagttccgtt taaataaaat aaaaaacttg tctctataaa ataattttaa
    13681 tcattttata aagacaagtc tttttatttt ccaatttaat tttattttct aaatttctta
    13741 cactcggctt gttttaaatt ccctttttaa gaataataat acaattatca tagcaataaa
    13801 atctgcaact ggctgtgcca accaaacacc atctaaatta aattgaattg gaagtacaat
    13861 gattaatggt ataaataaaa taaattgtct taaaagagtt aaaaacattg aatgtttaac
    13921 tttacttata cactgaaaat acacaggtcc tacaatagaa atccccatta taggcaatgt
    13981 aattagattt aggcttatac ctcttactgc tatatccatc aaatctgaat ctttattaaa
    14041 tatacctaca aatacttctg ggaaaacttg tactactaaa aatccaaatg acaatatgac
    14101 aactgacact atgatagata atattaaagt tttctttgca cgctcatatt gttttgcacc
    14161 ataattgtat gatattatag tttgcatacc ttgattcaat ccaaacacag gcatcataaa
    14221 catcaataaa atagatgtaa gtgctgtcat agctccaata gctaaatctc caccataaac
    14281 ctttagagaa ttatttgtaa acatgtgagt tatacttata gctacttcca tagcaaaagg
    14341 tgtcattcct attgcaaata ccttctttac tatatcttta ttcaatttta agttacattt
    14401 ttttagtttt aaattggact tccctcttat aaaataatgt attacccaaa agaatactac
    14461 tatttgacag agtactgttg ctatagctgc tccttttata cctaaattaa atacaaatat
    14521 aaatataggg tccaatatca ggtttaatac acatcctaca atcattgtcc ttgctgctaa
    14581 ttttggatta ccttctgccc taattgcatt attaagagaa aatgcaacta aattaaatat
    14641 acttccaacc aatatgacac tcatatagtc ttttgcataa aatattgaat ctttacttgc
    14701 acccaaaata aaaagtatat cttctaagaa aactaatcct aatatagtta taattaaaga
    14761 gataattata gatagtgtca aagcattacc taatatcttc tctgcttctt ctctatttct
    14821 ttcccctaac tttatagaca atcttgtgct agcacctact gataccaaca ttccaaaagc
    14881 tattattaga gtaaatactg gcatagttac accaagacct gcaatagcta tagagccaat
    14941 accttccata gaaccaataa aagctctatc tactacatta taaagtgaag ttaccatcat
    15001 tgctattatt gcaggtatcg aatacctaaa taataaaatc catatttttt catgtcttaa
    15061 tgcttcttga ttctccatgt tgtcaccatt tacaccatgt cataagttat acaaattggt
    15121 ttatttgttc ttgggtctgt tgctatttct gcatcaatat taaataattc ttttagatta
    15181 tccttcgtca taacatcata tgcatcacct tcacaaacta ttttaccttt cttaatacct
    15241 atcatgtggt cagcaaatct tgctgcatta ttaagttcat gtattaccat gacaatagtt
    15301 cttccttgtt ttttatttag ttcatctaat aattttagta tctccaattg atgtgccaaa
    15361 tctaaatatg ttgtaggctc atccagtaat aatatatcag tttcttgtgc caaagccata
    15421 gctatccatg ccctttgtct ttgtcctcct gataaatctt ccatatttct atccttaaat
    15481 tctgcaattc cagtaacttc taaactccaa tttacaatct ctctgtcttc tttactagaa
    15541 ttaccaaatc cacttttatg aggaaatctt ccataagaaa ttaattctga aacggtaaga
    15601 ccactaggag cttgagggct ttgtggaagt acagccatat cttttgctat ttctttagac
    15661 ttctttttgt agatgtcctc tccatttata tatattttcc cactattagg gctaataatt
    15721 ctacctatag ttttaagtat tgtcgatttt ccacaaccat tagcccctat gatggtagtt
    15781 atttttcctt ttggtatatt taaatttaaa ttttttataa tatctgtgtt tccatatgat
    15841 acatttagat tcttagtatt tatacagttc ataacaacac cctttcctaa tcatttacca
    15901 acattaaata aataaagtac ggcactccta ttatagaaac tactatacct actggtattt
    15961 ctataggtgc tagaagattt cttgatatag tatctgctat taaaagtaat aatgttccta
    16021 tcaatgctga tactggtatt aatcttttat gctttggtcc tattattttt ctacctatat
    16081 gtggagctat taaacctaaa aatgaaatac tacctgctac tgctgtcgat acaccagata
    16141 aaattactgc atatattata agtcttcttc tttgtttttc tacatctaca ccaagactta
    16201 tagatacttc atcacccaaa tttaaaacat ctatatattt agatttataa atagttatac
    16261 ccataaaaat cactataaat ggaagcacag ctattacata tttccaactg gttccccaaa
    16321 tgcttcctga tgtccatgcc ataaccctat taaattcctg tgttgtaaat ttcaattgga
    16381 atattgtcaa aattgaagta aaagctatgt ttacacctat accaataagt agtaatcttg
    16441 atgaatttat accacctttc catgcaagag aatatataag aaatgctcca aataaagctc
    16501 ctataagtgc aacaataggc attgtaaata cagtcaagtt actcatacct tcatatacat
    16561 taccattcat aaggtatata taaactacta caaacaatgc agcacctgaa tttattccca
    16621 aaattccaga atcagctaaa tcattctttg taactccttg aagtattaca cctgctgttg
    16681 ataatgcaga accaaccaaa agtgctatta ctattcttgg taatcttaat ttaaatattg
    16741 caatttcatg attacgctga ccctgcccta aaaaagtctt tataacatcc attggttcta
    16801 tagaaaatga gcccatattt aaactcaata aaaaagtgat aaaaataagc accattaaaa
    16861 ttgatataac aagtaatgtt ttagaattta tttttataat atttttcata gtacctattt
    16921 ctcccccttt ctaacaaggt atataaatat tggcactcct ataagtgctg ttatagaacc
    16981 tactggtgtc tcatatggag gatttatcat cctacttaat atatctgtat atacaagcaa
    17041 tacagctcct aatactaaag agctcggtat tatatactta taatctgaac ctactattcc
    17101 tttagctatt tgaggaacta taagacctat aaaaataata ttaccagaca ctgacacaga
    17161 tgctccagtc attaatatta caagtactat acatataaat ctaacaaagt tagttttttg
    17221 tcctaatcct attgccactt cttctccaag acttaatata gttattcttg gagccataat
    17281 aatagctaaa ataaatccaa taccaccagc tacaaatagc aattttatac cttcccactt
    17341 cactcctaca agccctcctg atatccagaa acttaattgt tgagataggt taaaatacat
    17401 agatattgct gatgccaaag aaattagcaa tgtaccaaga gcgactcctg caagagctaa
    17461 ttttacatta ttaactcttc ctcttgattt agaacttata aaatatataa ataatccact
    17521 tatacttgct ccaataaatg caaacatcat tacactaaaa cttccatgta taagttgagg
    17581 aagttttttt tgaagtacca gtgatatcga aattgcgaat gtagctcctt gtgtaattcc
    17641 tataacagat ggttcagcta taggatttct cgttattcct tgcattattg ccccagaaac
    17701 tgctaaaaat cctccaacaa gtgcagctgc tattgccctt ggaagtctaa catcctttac
    17761 tattctcata tttattccat cttccatttt tatcaaacta tctataacag ttgatagatt
    17821 catatctttt gccccaatag ttatggataa tacaattcca aatacaagta caactatact
    17881 tactaatatc aacaaataag ttatattttt tttactcagc ttagtcaatt atcctacctc
    17941 cttttccctt ttaatcgtca gtataatatc atctattaag tattttaatt ttaagattta
    18001 ttaaaaatag gtgtgaaaaa tgaatatcat tatctcacac ctactaagtt aagaattact
    18061 ttgttaattc atttttaact gattctaata ataactcttt tccaattgga ttgtatgatt
    18121 gactgaaaaa tggagcagca tcaagtattg ttacattacc atcttttact gctcttattt
    18181 gagcccagat agcactattt tctaaatctt ttttatctgc ttctgtagca attacaacta
    18241 tatggtctgc atctatatct gttaaacctt ccatacttac cattggtagt gttattccat
    18301 cctgttttgg catattctta ggtttagcta atttcatatc atcattgatt agagtaccta
    18361 ttccaccatc actaaatacc ataaattgac ctgaacttgc caacactggt aaataagttt
    18421 tttctccatt cttttctata acttccttac ctaactttgt agctttttca tcatacttct
    18481 gtagccaact tttagcttct tcttctttgt caaataactt tgaaacatca gttaacttgc
    18541 ttctccagtc atttgcataa tctttcatca taactacagg agctatttct tttaattggt
    18601 catatatttt ttcttgtctt tgactcatta ttattaaatc aggatttact tctaatatag
    18661 cttccatatc catagtatcc atcattgagt gcccaactat ctttacacct ttaagctctt
    18721 ctcttatgta acttggtaat ttatcagttt catatgagtc tacatttgct gttgcaacag
    18781 gcttataacc tgctaataat agttcttcac tacttcctga tatatcaact atttttttag
    18841 gatttgatgg tatcttaacc tctcctttaa cagattgtac aactcttgtc tctgatttat
    18901 ctttatcctc tgtattctta tctgaacatg ctgtaacaag tactaacgta gttataatag
    18961 atataaatat tgcaagtgat tttatttttt tcatgattac ttctcctact tgtttattat
    19021 acttacatga tattgattat cataagcaca tatttattat agcatttttc tatttttagt
    19081 caatattatt ttgtaaaagg ataaaaattc ctaattaatc ttaattatat atagcttaga
    19141 taaggttttc ttatattaaa cagtattttt ttaaagaatt ggacacaaat acaatgactt
    19201 ttcagttaaa caatgataaa atgcaattta tagataatga atttagtaag gaggattcaa
    19261 aacatggtac atttagtata ttgtgataat gcaggaaaaa aaggagaaaa ggtcttagat
    19321 aaaatcttgg ctggaacaaa aacaatggtt attagaggtg ctgctggtag aaaaatacca
    19381 catagtagag tatttgaaga tgagttactt tattttatgg aaaaaggaac tgcaaaaata
    19441 tctgcaaaag ccactgttaa aaaggtagaa aactatgtta aattaacaga agatgaaatt
    19501 tctaaaattt taaatgaaaa tcaaccaaaa ttaaatttat ctgaaaagca aaaagttcgt
    19561 tggcataaaa aatgtttatg cttagttgaa tttgaaaatg ttgaagaaat aacacctctt
    19621 gattttgacc atcaaggaaa tatggatgat tggcttattg ttgaaaaaat tgaagatgta
    19681 gttgttggta ctagtatacc ttacaattat gagaagtcta aattttaaag tatcaataac
    19741 ataatactat attgctaaac tctacttaaa taaaaaaaga gtgcatttaa taattataaa
    19801 aattattttt atacactctt ttttaataat aattttaaat tacttacttt ctaatctttc
    19861 atatacatct ataaattctt ctgctaattg ttgaaaattc atagctgtca ttaaatggtc
    19921 ttgtgcatga ataagtataa cacttatttc agttttatta ccattagctt cattttgtat
    19981 caattctgtt tggaatttat gagctttatg aattgattct ttcgactttt caatacattc
    20041 tctagcttta tctatattcc cgctctttgc ttcttttatt gcttcaaatg ctagcccttt
    20101 cgcatcacct gcaaatccaa ttattccaaa agatatctct atcatcttct catccattta
    20161 ttcgacctcc taaatatctc tatagcttga tatgactaat ccattctttt ctaaaaattc
    20221 tttgactttt ttagatgtaa gtatcttgtg ctctttcgtt ctatcaatag cataagatgt
    20281 agaatttaaa atataatcat ctaaaaaagc tgggtgagac ataatatcca ctacatcata
    20341 cttcattatt tcgtctatat tttttataaa atattcttca cttacatttt ccttataaaa
    20401 actatctatt aaaggaacta ctttagaata ctctaaatta tattcaaatc cacctcttat
    20461 tggaagatta tacttggtta caatcttctc tattactggc tttaaactta ataaagtatg
    20521 gatatgatgg tgactatcta aatgagatat atctattcca agccctttta ccctatctat
    20581 ttgagcacaa agttctctat aaatctcatc acaatccatt ttttcaataa tttcgtctgt
    20641 aatcctccta ataaaaaaac cgtctttatc aacaatagtt tttaagttag ataataatgg
    20701 tctaccacaa cttaaagtca tatgaactcc acaatttaaa gttttatttt ctttaagtaa
    20761 gcctactcca tgttctaccc caggcatatt tgccatcata gaagtagacc taactatacc
    20821 attattatga gctgaaatta ttccatagtt cacagcttca caatatccaa aatcatctgc
    20881 atttattata attttagtca taccataacc tactttctat agacaactat ctattaaatc
    20941 taggaagata atctttatga gcttccaaca tttcgtctaa tatagttttt gctaagtcat
    21001 cagatggaat caatgggttt atacaaagtg ccaaaagagc agattcataa tcgccatata
    21061 cagctgcttt agctgccaat acttcaaatg atttgatttg acttactaat ccatgcacag
    21121 aatctggtaa atatcctata cttattggtt ttggaccatt ttttgttatt acactactta
    21181 cttctaccgc ttgttcatct ttaaaatctc ttatagaacc cttatttaat gtattaacta
    21241 cttggatatc ttttttgtcg ttgtatatag aacttattaa attacaagca gcatcactat
    21301 agtaagcccc tcctcttttt tcaagttgag gtggttttac atctaatttt tcatccttat
    21361 ataattcaaa taattgttct tccaattctt taactacttg acctctagct tttccctctt
    21421 taaactctct caactcatcc tcaagcattt ctttagtttt atagtagtat ctatgatatg
    21481 gacaaggtat tgcacccaaa gattttacaa attcagcatt aaaatcaatt gcctttatat
    21541 tttgcatact tatatctgct ttgacaaatt tatcaattgc ttcttttgta acatcttctc
    21601 catctaaaga tacatttaga ccataaacca tatgattaag acctgcaaaa tccatactta
    21661 ttctgtcact ttctacatta aatagtttgg caacatcatt ttttaaatga attggcacgt
    21721 tgcatagacc tatatatctt ctaaaattag tatatttgaa tacagcctct gttattattc
    21781 ctgttggatt tgtaaaattc accatccatg catttggaca taattcttcc acatctttta
    21841 ttatatcaaa tataactggt attgtgcgaa gggctttaaa taaacctcca gcaccattag
    21901 tttcctgccc cataactcca tgagataaag gaatagtttc atcttttatt ctcgcatcta
    21961 aaagacctac tctaagttgt gttgtcacaa agtctgcatc tcttagagct tctcttctat
    22021 ctaaagtaag atttattttc atatctactc cagctttttt taccatcctc ttagctagat
    22081 ttccaactat ctctaatttt tcttttccag cttctatatc tacaagccat aattccttta
    22141 caggtaactc ttcatatcta tttataaaac cctctacaag ctcaggagta taacttgaac
    22201 ctcctccaat tgtaactatt tttagtccct ttttcatctc attataccat ccttacctat
    22261 aatttaatat actctacaaa accccataca tttatttaaa actctataca tttatttacc
    22321 gaaataaaac tacctagttg ttaattttca atttattagc ttccattttt tcagacgcta
    22381 tgacaaatgg aatatatatt aatattgata atattaaatt aaatagtgct aaagctccac
    22441 cagtccagtc acctgtagcc attgctccac ctaatatagg aggtgttacc caaggtattt
    22501 taggtaaaat tactggtgct actaatccaa aatctattgc taagtatgat acagcactac
    22561 atattactgg tgcaagtaca aatggaatca taaatatagg atttaaaact attggaagtc
    22621 caaatatcaa tggttctcct atgttaaata ttgcaggtgg accacctaat actatcatct
    22681 ctttacgtct tcttccagct attaataaag ctactattaa tcccaatacc attccagaac
    22741 cacctaaata tacaaatgca tcaaagaatg cacctgcaag tacatggtat ccttccgttg
    22801 ctcctgcttg agccaaggca gcattttctc cacctaattt cataagtata gtttctgtaa
    22861 aaggaagtgc tatatttgca ccatgaagtc caacagtcca aagtatatga attataaatg
    22921 caataagcat tgtagaacct agactatcag ctacatttga tagtggtgct cctaaataag
    22981 tatttaaaat atctgttaag aaacttccat tagaaagcat ttttataaat attcctatta
    23041 cagtaaatat cattatagtt aacattcctg gtattaactt tgcaaatgat cttgaaactg
    23101 ctggtggaac tccatctggc atttttatag ttaatttctt taccttagat aatcttacaa
    23161 atatctcagt tgataataat cctattaata tagaagagaa taaagctgct gtacctaagt
    23221 aggtttgttg tatcattccc catccttcaa ctactgtagt tcctccctca ggaactattt
    23281 taccaatttg tggagccatt ataaaaaaga tagataaagc tataagccca gattgtaacc
    23341 catcactctc ataagatttt gctagaaaat aagcaacacc tattactaag aatactgcca
    23401 ttgtacctat tgctccccac caaaggtcgc ctccaaatgt ggtccagttt tctccaaata
    23461 tacttgccat caaatttttg tatgcttcca atggtaagtt atttatcaat gttcccaatg
    23521 cacctaccat agttataggt atcattacta taaatgcatc tcttactgct actaagtgcc
    23581 tttgagctcc tattttagca gctacaggca ctatatattt atccataaat gacatgaatt
    23641 tttccatttt acatcctcca gtacaacttt attttgaact atttaactct acagctctat
    23701 ctaacacagc ttttccatta catcttccat aatccatcat atttataact tctactggaa
    23761 tattatatgg ttttgccatt tcagttgctt ttcctaatac atatctaact tgaggcccta
    23821 aaagtaagac atcacaattt tctatttcat tttttaagtt cacttctgat atagcccata
    23881 tttttgcttc tataccgttt tcctttgctg catcctccat ttttgtaact aataaactag
    23941 ttgacattcc tgctgaacaa gctaacatta tttttatcat tttttcccct ccaaattaga
    24001 ttcattaaat attttttaag tcataagttt tttaaactat acatttaaaa tatgtttttc
    24061 tattaatttt ttcttaatgt atatatctta tttttaaatg tatatacatt taattcttga
    24121 tattattata taccaaatgt atatacattt caagaaaact ttttcatttt atttaaattt
    24181 tattcgacaa cagcattcaa agttaatgta tatccatagt gatatgtgtg tgagtactca
    24241 aaaatctcac tattttgtaa cttacaaatt tggcttatat ataaaagtgg ctctggttct
    24301 tctaatttta gattatccat acactcttca ttcgatatta cagcttgtac ttctctctct
    24361 gacatggcta tcttgtatcc acacacttct tcaacaaaat tataaagaga attttcacaa
    24421 tgagtcttat ttaaatttgg aaataactta actggcatat aagtaatcat atatgaaact
    24481 cttacatcat tagctaatct tactcttttt atttcccaaa cattttcttc taaacttata
    24541 ttcaacttac ttgataaatt ttcatctgcc tgaactattt caagagatac caatatatta
    24601 gatactttat agcctttact ttccatctct ttagtaaatc cacttacaga cgatatatta
    24661 tcatatatat tatttgcaat aactatacta cctacacctc tttgtctagt tatatagcct
    24721 tctttaacta gattattaag tgcagacctt acagtcattc tagttacatt aaattcttca
    24781 ctcagctgtt tttcagaagg gattaaatcc ccttctttca aactgtctga atttattaac
    24841 tctctaacat gattttctat aactttataa attggctctt tcatttaatt cactccttat
    24901 taaatctttt atataataag ataattttat atacaaacaa agaaaatgtc tatacattta
    24961 agataattta aattttattc agaaatcttg tacaactttc cgagttttaa taaagatata
    25021 aatagactta tcattaatat accttttaat atacttgata aggttatact ccaccataca
    25081 ccatttagac ccaacatttc tggtctagat ataaagtaag ccaatggtac tcttgcacca
    25141 gtcagtatag taattataat agaaggtata taggttctcc ctaatccttt gaaagctcct
    25201 gttgttatta tttctaaaca cataaaaaat tgagaatagc ctaatatttt aagataaact
    25261 gctccttttt ctattgcctc actttcatct ataaaaatag aaaatatact ctctcctgca
    25321 aacactaata ctaaagtagt tattatacca agtacagaag atacagttat agctattttg
    25381 caacctttat ttattctaga atacttcttg gcaccataat tttgtccaac aaaactgcct
    25441 aatgcaactg atagcccttc agccgtcatc caggaaattg cttctatttg tgagcctact
    25501 ttttgtactg caacagccac aggcccccat gatgctacta ttactcctaa tagcattgag
    25561 aatactgtaa acataccact ttgtattgct ataggtaatc ccaatttata cagaacttta
    25621 taatatttag tctctatttc tttaaatagt cttaatttaa aatattcttc tttactttta
    25681 actattatat atataaaaca tgtagtaact actatctgag caaatacagt tgcaatagca
    25741 gctcctgcaa caccaagttt tggagcaaat ccccatccaa aaattaatat agggtcaagt
    25801 ataatattag ttattaatcc tactgtatta atacgaaaag gagttttact atttccaaga
    25861 ccattgaaaa ttgcagtaaa tactggatta ataaaataaa ataccatacc tagagctact
    25921 attatcaaat actgtctaga catggttata acttctaagt ctcctaatct aaaaaaacct
    25981 atcaattgtt tattaagtac tattaaagaa attgtatata ttattgctag tataatattt
    26041 atttctattg ctgacttcat atatgattta gtagtgctta tattatttgc accaatactt
    26101 tgtgctactt ttatctctcc acctactttt gaaatcataa taaatgccat tgcaagccat
    26161 ggaaaaaaac cagctgttcc aactgcagct actgctttac ttccggcttt accaacccat
    26221 atcatatcta tcatgttata ccccatctgt ataaatgaag tccccattat tggaagtgat
    26281 agcttaagta atttttctgt aattcttcct tctgttaaat taagtctgtc tttcattttc
    26341 cacctctttt atattaaatt tatttgctta tatagctaga ttaatcattt tatttgattg
    26401 agttcactat ccttcaattt atattatcat atctcaggat tgcatctcaa tcatacttat
    26461 gtttaagata cagtatttta cctatctatg ataaaattaa tacttatgaa tataaatcta
    26521 gccatttcat caatacatat tctaaccttt aacttaactt caattaagta tacatatcgt
    26581 aaccaattag aatattttat acggtatctt ccattaataa acaaccaata tcaatgattt
    26641 atatataatt ttatttatac ataaaatagt atactataat aataaaaaaa ctatatttga
    26701 atgctttaag catgtttact aattgtaatt tccacacaaa tttgataata tatgaatata
    26761 ttatggtgaa atttatagat atatattaat tatggggaga ttgtgggagg ttcttttcac
    26821 aagacattta agctatgaaa ataaataaaa aattcaatag agttgatatg caggtttcaa
    26881 tacttactgc aattattgta ataatatctt cctcatgtgt atattttttt aattatacac
    26941 taacttataa gagcatgata tactctttaa gtgagaggtc aaaacatatc tatgaatatg
    27001 tagaaagaaa tattgataaa aataccttta ctgaaattaa ttctaaagaa gaccaatcaa
    27061 aaaagtctta taaaagttct aaaatccttt tagaatctgt aaaaaatact actggagtca
    27121 tgtatctata tactgctaaa aaaacaaaag atggatcact agtatatgtt gttgatggtt
    27181 taaattcatc taggagtgat tttagaaatg ctggtgattt gatagaaaaa gagatttggg
    27241 gagaccttaa caaagcatta gataatcata tattgttacc taaaaagatt aaatcaacat
    27301 cttggggata catatttata tcgtattttc ctattcacaa tgatgttggt aaggttgttg
    27361 gagtaatagg aattgaattt gaagcaaaac atcaatacaa tacatttaaa tttataagta
    27421 tagttacacc aataatagca ttattaactt gtctaatatc agcattgatt gcagtgattc
    27481 tatttaaaag aatatctaat cctacatata ctgattttgc taatacagat tatttaacag
    27541 gcctaaaaaa tagaaatgct tttgaaatag atatgaataa cttaaacaat tcaaatctaa
    27601 agaatctaat atctatcata tcaatagatt tagacggttt aaaaaagatt aatgataaat
    27661 ttggccatca gacaggagac ttatatatta agcaagcaag taaaattata ca
//
LOCUS       DAYAOY010000049        27640 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYAOY010000049 DAYAOY010000000
VERSION     DAYAOY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.049"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            452..1876
                     /locus_tag="ACGI6B_002212"
     CDS             452..1876
                     /locus_tag="ACGI6B_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFG9751654.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1902..4040
                     /locus_tag="ACGI6B_002213"
     CDS             1902..4040
                     /locus_tag="ACGI6B_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751655.1"
                     /translation="MDNLKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVY
                     LKGQEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IRKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     VVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            4070..4492
                     /locus_tag="ACGI6B_002214"
     CDS             4070..4492
                     /locus_tag="ACGI6B_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9751656.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4511..5662
                     /locus_tag="ACGI6B_002215"
     CDS             4511..5662
                     /locus_tag="ACGI6B_002215"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9751657.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5768..5869
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            6052..6624
                     /locus_tag="ACGI6B_002216"
     CDS             6052..6624
                     /locus_tag="ACGI6B_002216"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFG9751658.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6688..7338
                     /gene="fsa"
                     /locus_tag="ACGI6B_002217"
     CDS             6688..7338
                     /gene="fsa"
                     /locus_tag="ACGI6B_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFG9751659.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7490..10414
                     /locus_tag="ACGI6B_002218"
     CDS             7490..10414
                     /locus_tag="ACGI6B_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9751660.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            11003..11461
                     /locus_tag="ACGI6B_002219"
     CDS             11003..11461
                     /locus_tag="ACGI6B_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9751661.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11522..11797
                     /locus_tag="ACGI6B_002220"
     CDS             11522..11797
                     /locus_tag="ACGI6B_002220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9751662.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11856..13130
                     /locus_tag="ACGI6B_002221"
     CDS             11856..13130
                     /locus_tag="ACGI6B_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFG9751663.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            13260..14312
                     /locus_tag="ACGI6B_002222"
     CDS             13260..14312
                     /locus_tag="ACGI6B_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9751664.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14312..15343
                     /locus_tag="ACGI6B_002223"
     CDS             14312..15343
                     /locus_tag="ACGI6B_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFG9751665.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15435..16247
                     /locus_tag="ACGI6B_002224"
     CDS             15435..16247
                     /locus_tag="ACGI6B_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFG9751666.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            16250..17170
                     /locus_tag="ACGI6B_002225"
     CDS             16250..17170
                     /locus_tag="ACGI6B_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFG9751667.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17345..17791
                     /gene="rpiB"
                     /locus_tag="ACGI6B_002226"
     CDS             17345..17791
                     /gene="rpiB"
                     /locus_tag="ACGI6B_002226"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFG9751668.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            17821..18486
                     /locus_tag="ACGI6B_002227"
     CDS             17821..18486
                     /locus_tag="ACGI6B_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9751669.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18596..20098
                     /locus_tag="ACGI6B_002228"
     CDS             18596..20098
                     /locus_tag="ACGI6B_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFG9751670.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20410..22002)
                     /locus_tag="ACGI6B_002229"
     CDS             complement(20410..22002)
                     /locus_tag="ACGI6B_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9751671.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(21971..22687)
                     /locus_tag="ACGI6B_002230"
     CDS             complement(21971..22687)
                     /locus_tag="ACGI6B_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751672.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(23227..24027)
                     /locus_tag="ACGI6B_002231"
     CDS             complement(23227..24027)
                     /locus_tag="ACGI6B_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9751673.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(24341..25072)
                     /locus_tag="ACGI6B_002232"
     CDS             complement(24341..25072)
                     /locus_tag="ACGI6B_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HFG9751674.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(25059..25754)
                     /locus_tag="ACGI6B_002233"
     CDS             complement(25059..25754)
                     /locus_tag="ACGI6B_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9751675.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(25756..26547)
                     /locus_tag="ACGI6B_002234"
     CDS             complement(25756..26547)
                     /locus_tag="ACGI6B_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751676.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(26557..27369)
                     /gene="modA"
                     /locus_tag="ACGI6B_002235"
     CDS             complement(26557..27369)
                     /gene="modA"
                     /locus_tag="ACGI6B_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9751677.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT        10848 a         3109 c         4680 g         9003 t
ORIGIN      
        1 attgccttat agggtaaagt tgtatattaa ataatgttta gtgtttaata gtagctaatt
       61 taaaattaat ctttagtgat tcatataatt acatttgtat agtttacatt gataatatta
      121 ctagagagtg cataaacctc aaaaatacag ataaattaaa tctatattag tatatccaaa
      181 agtacattta ataaatttat ttaactgaat tattattttc atttctatta aatttaaata
      241 ttttataaaa atagtattat ttacaaaatc atatactcca aaatactaca taaataaaat
      301 taaacaaaaa ctaaaatgac aaatgtcatt atctaaagtg acatagaaac ggtttcttaa
      361 agttgttact ataaaatata aactctataa taaaagtata acaaaaggga atcatatcga
      421 tatagaatat aattcaagag gaggtaaaat tatgagtagt tctaatcaat cgacaataaa
      481 gacatctata cagcgagtag ggaaattttt aagtggtatg gtaatgccaa atataggagc
      541 ttttatagca tggggtctta taacagcatt atttatacca acaggatgga tgcctaatga
      601 aaaattgagt actataggtg acccaatgat aaaatacctt ttaccactat tgatagctta
      661 tactggtggt aaagcaatag caggacaaag aggtggcgta ataggtgcag cagcagctat
      721 gggagtaatt gttggtgcag atataccaat gtttattgga gcaatgataa tgggaccatt
      781 tgcaggatgg gtaattaaga agtttgataa atttgtagat ggaaagatac caacaggatt
      841 tgaaatgtta gtaaataact tttcaatagg tataataggt atgcttcttg caatactagg
      901 attttatgca attggaccag caatagtagc agggacagct cttatagaat ctggagttca
      961 atttatagtt agcaaaagtt tattgcctct agtttcagta tttatagaac ctggaaaagt
     1021 attattctta aacaatgcaa taaatcatgg tatattaggg cctataggta tagctgaggc
     1081 aaaagaagca ggtaaatcta taatgttctt actagaaagt aaccctggac caggtttagg
     1141 agttctatta gcatattgga tgtttagtag aggaagtgta aaacaatcag caccaggagc
     1201 tgtaataata catttctttg gtggtatcca tgaaatttac ttcccatata ttttaatgaa
     1261 tccagtttta atattggcta caatggcagg tggagcagca ggaatattga ctttttcaat
     1321 attaggagct gggcttgtag cagcaccatc accaggaagt atatttgcac ttatggcact
     1381 agctccaaag ggaggattac ttccagtatt agcaggagtt gcagtggcaa cagttgtatc
     1441 attcttagtg gcagcaccat ttgttaagag agcttcagca aatcaaagtg aagaagacag
     1501 tacttcttta gaagaagcta aagcaaaaat gagtgatatg aaatctgcaa gtaagaactc
     1561 tgagaaaaat attgaggaaa aacagcttga agtaaatgag attaagaaga ttgtatttgc
     1621 atgtgatgca ggaatgggtt cgagtgcaat gggagcttcc agatttaaaa atagaataaa
     1681 aaacttagac ttaaatatag aaattacaaa ttcatctgtt gataatcttc cagatgatac
     1741 acaaatagta gtaactcaca atacattagt tgaaagagtt gctaagaata attcaagtgt
     1801 tgagatagtt tcaataaaca attttttaaa tgacccaaat ttagatacat tgtttaaaag
     1861 attagaatct aaataaaaat aaaaagtacc actaattagg agtggataat ttgaaaaaaa
     1921 agaagattac atctaggcaa aaaaagataa tactaatgat agtagcaaat tcaaagaaaa
     1981 acattccaat aactatttct gagattgcag ggactttaga gttaagttca agaactgtac
     2041 ttagagatat gtctggaatt gaaaaatggt ttgatgaaaa tgattttaac tttgtaaaaa
     2101 aaccaggagt gggattgatt ttagaggaaa atatagaaaa tcaaaacttt ataattgagt
     2161 tgttagaaga agaaaaaata gaaaaagaat acagcaagga agaaagaaat ttaattatat
     2221 tatcaaagct actagtttct aacgagccag taaaatccta ttattttgca aaaatattaa
     2281 aggtatctga atgggtactg aataatgatt ttgccttagc aagtaaatgg ttggaaagat
     2341 ttgatattga gcttgttaga aaaccagggc tcggtgtcta cttaaaaggt caagaaaaaa
     2401 actttagaga ggcatatgta aatcttatat acgattcatt caatgaaaaa gaaatactag
     2461 atatggtcag aaatataagt gaaaatatac aaacagataa agcaatagaa attttaagtg
     2521 aaaatagact tttaaattta atggacaggt gcataataag aaaagttgaa gctacattaa
     2581 caaagaagct ttcagattta gatgtaaatt tagctgatag tgcatatata gggcttgtag
     2641 ttcatatttc attagcctta cagaggataa aaaatggtga aaatataact atggataaag
     2701 agtttctaaa agaattatct ataacagaag agtttaaatt agctaaagaa atcgtaaaag
     2761 gtatggaaac agattttaac atggatatac cagtagatga ggttggatat ataactatgc
     2821 atataagagg tgcaaagcaa aggtcatctt caaaccataa ggctttaaac ttagatgata
     2881 tagaaataat ggaaattaca aataagatga tagatttggc ggaagatgaa tttaaaataa
     2941 gtttaaaaaa tgacgagagg ttatttaaag acctagcaaa tcacttaggg ccttctataa
     3001 ataggttgaa tatgggatta gaaataagaa atcctctttt agatgaaata aaaagcaagt
     3061 attcatatgc ctatgatgta gttgaaaaga tttctagaat tataaaagat aaactaaata
     3121 taaattcaat acctgaatca gaaataggat atattgcaat gcactttgca tcagccatag
     3181 aaaaaaatct tatgatgaat actaatataa atattgttgt ggcatgtccc actggaatag
     3241 gtacttcaag attcttatca actaagattg aaaataaatt tcctaattta aatatacttg
     3301 aaaccatatc agctataaac atagatgaag agtatttaaa ggaaaaagat gttgatttaa
     3361 tagtgtctac agttgagtta aatacaagtt taaattatat atgtgtaggt ccatttatga
     3421 gtttagatga tgaacagata atcaaggaaa aaataaaatc tatagctcaa aataaattaa
     3481 tcaatttaaa tgcgaaaaat gatactaaga gtaagaataa agtatatgaa cagataacag
     3541 aaagtatgaa tataggaaaa gatattcttc aatttttaga ggaaattaga tttgaaaaat
     3601 ttgaaagtaa agatttaagt gagcttattg aagactcatc aaaaatattt gcaaaatcta
     3661 gtgaagatat aatctctata aaagaatcgt taaaagaaag attaaaaatt tcaattccat
     3721 atatagaaga atcaaaaatt ctattgttac attgtatgag tgaaagaata gatattatga
     3781 agttatcaat aataaaacta gagaatagaa tcgtgctaga ttcaaatgaa gaaatcgaca
     3841 atgtagtttt tatgttactt cctaaaaatt caccaagtta tcaaagacaa ataatgagtg
     3901 aaataagtgg ttcattaata gacaatataa tttttactaa taagataaat aaattctcta
     3961 tagaagagat ggcattagag gtaaaagata tagtttttaa tttttataca aacagattaa
     4021 aagcatttat agataaataa aattaatatt aaaaaggaag gttgatacta tgagtaaagt
     4081 attgaatgag aataatatat ttttagggtt agatagtgtt tcaaaggaag aagctataac
     4141 tttagcagga agaaaattag ttgaaaatgg atatgtaaaa gaagaatata taccagcaat
     4201 gttagaaaga gaaaaagtaa tgactacata tatgggaatg ggagttgcta ttccacatgg
     4261 tgtaaatgaa gcaaaaaaag aaatattatc ttctggaata gttatattac aattccctaa
     4321 tggtatagat tttgatggag aaaaagcata tttattaata ggaatagctg gtgttggaga
     4381 tgaacattta gaaatactat ctaatatagc agtagtttta gatgatgact taacagaaag
     4441 actaaaaaat tcaaatgaca aacaagcttt tatggaagct tttgccaact aatcttagga
     4501 gggatttaaa atgaaaaagg caattcagtt tggagcagga aatattggaa gaggttttat
     4561 tggaggcttg ttagttaagt cgggttatca tgtagtattt gctgatgtaa atgaggagat
     4621 attaaactca ataaacaaag ataaaaagta tactatacac ataagagatg tagaatgtat
     4681 agatgaagta attgataata taagtgcggt ttcatctata aaagaagaaa taatagatga
     4741 gattgtacag gctgaaataa taactacagc agttggacct ttagttttga ctaaaatagc
     4801 ctctacaatt gcaaaaggta taaaggctag aaaagaaaag ggattaacaa gtaatctaaa
     4861 tataatagca tgtgaaaatg caatatacgc aagttcttct ttaaaagaag aggtacttaa
     4921 gtatttaaat aaggaagaag tagaatattt agagatgtat gttggttttc caaactgttc
     4981 agttgataga atagttcctc ctggtaaaaa cgaaaatcct cttgatgtaa cagttgaaaa
     5041 tttttatgag tggaatgttg aaaaacaagg atttaaaggg gaaataccaa ctatagttgg
     5101 tatgaattta gctgataatc ttatggctta tatagaacgt aaattgttta cactgaatac
     5161 aggtcatgct ataacagctt atataggata tttaaaagga tataagacta tagaagagag
     5221 tataaaagat aaatttatct gtgatatagt caaaagtgca atggtagaaa gtggagaagg
     5281 attgattaaa aaatacaatt ttgactcaga agtgcactat aaatatattg ataaaatttt
     5341 aaatagattt aaaaatccat atcttaatga tgatgtatta agagtcggaa gggaaccatt
     5401 aagaaaacta agtgataaag atagattgat taaaccttta atgacagcaa aatcatatgg
     5461 attatcagtt gataatttga ttcttggaat aggtgcagcg cttcattata ataatagtga
     5521 agatactcaa agtgtagaat tacaagagtt gataaagtct attggagtaa aaaaagccgt
     5581 tgctaaaata gctaatataa gcaatgacga agaactttta aacaatatcg aaaaaagtta
     5641 tatatttatg aaaaatttat aaatttactt gaaaaaaagt agttctgata aagtaactat
     5701 aatatattat tctacacagt ttgaattatt tgttgaataa tagagaatta taatatataa
     5761 ttaaaaggaa taaattaata tatttacctg tatatgtttg ataatatggt tcaaatgttt
     5821 ctactaagct accattaata gcttgactac aggagtgaag aagatatagt ttatacttat
     5881 tttaaattat atattaagtt aattactttg aattttaagc tcctgtctat gatatagaca
     5941 ggagcttttt tagtatttta atataaaata cttacaagaa tagatagcaa aattagaaca
     6001 aatataattt ttttattaat ataattttag aaagggctag gtggaagttc aatgaaggct
     6061 ttacaagaaa agatattgag agaaggcagt gtttcaggaa atgatatact aaaagttgat
     6121 agttttttaa accatcaaat tgatgtagct tttttaaatg aaataggaag agaatttaaa
     6181 gagagattta aaggagaaaa ggtggataag atatttacaa tagaggcttc aggaatagct
     6241 atagcatcaa ttgtttcaca gtattttgat aatgcacctg tagtatttgc aaaaaaatca
     6301 gaatctaaaa atttggataa agatgtatat gaaactaatg tatattcatt tactaaggca
     6361 agagagtatt cagttaaagt ttctaaaaag tacataaaca aaggcgaaaa tatattgata
     6421 gtggatgatt ttttggcaaa tggaagagct gctcttggac ttaaagattt aattgagcaa
     6481 gcagaagcta acttagttgg agtaggaatt gtcattgaaa aaggatttca agctggagga
     6541 gcacttttaa aggctaatga tgtaagatta gagtctttgg cagtagttga atctatagat
     6601 aatggaactg taaaatttag gtaatttatg atattatata cttgtgttta gcacatattt
     6661 taactatgta attatggagg attttttatg agatttttta tagacacagc caatattgaa
     6721 gaaataaaag aagcaaatga tttaggtgta atctgtggtg taactactaa tccgtctcta
     6781 atagctaaag aaggtagaga ttttatagag gtagttaaag aaataagtga aatagtagat
     6841 ggaccaataa gtgcagaagt aataagttta gaacataaag gtatgataga ggaagcagaa
     6901 aaattatcta agatacataa aaatatagtt ataaagatac ctatgacagc agaaggtcta
     6961 aaagcggtga aagtactatc aagtaaaggt ataaagacta atgtaacgct tatattctct
     7021 gctggacaag ctcttttagc tgctagagct ggagctacat atgtaagtcc ttttgttggt
     7081 agattggatg atatatcaca aaatggtctt gatttaattg aagaaatcgt tgatatattt
     7141 agtgtaaata gtattgaggc tcaaataata gttgctagtg ttagaaatcc aatacatgtt
     7201 ttacaagctg ctagaatggg agcagatata gcaacagttc ctcttaaagt tataaaccaa
     7261 atgattaagc atcctttgac agacaaagga atagatagct ttatgaagga ttgggaaggt
     7321 gcttctttaa aattataatt ataaactacg ctttataagt atattaaaga ggttattatg
     7381 gaaattctaa gtaataaggc taggctattt tgcctagtct tattttggtg tataatatat
     7441 tgaaacatat tattgaaata gattaatttt ttaagtgagg aggcgaaaaa tggataaaat
     7501 attaagagag atacaaagag aagataaaaa aaatccatat actgaccaag aacttgctga
     7561 aatgttaaat gtagctaggt ctgaagtgat atcagttagg aaaaaaaata atattttaga
     7621 ttcgagagaa agaagaaaaa aaatattaat aaaagatata tcaaaaattg taagtgaaaa
     7681 tcctcaaatg tctgaaagaa aaataactga aatattaata gaaaatggat ataatatatc
     7741 gagaagtgca gtttcaaaat tattaaaaga ggaaaattta aatcagtcaa agattgggat
     7801 taaggataaa gacccagcat catcaggaaa gtatgtgaag gtgagtaaag ttgtagaaga
     7861 agatggattt gaagaattga taggtataaa gggaagtttg aaagaaaagg taaatctagc
     7921 taagtcagca ataatgtatc ctcctaatgg ccttcatact ataatatatg gagaaacagg
     7981 agtaggtaaa agtgaattag ctacatgtat gtataaatat gctataaaaa acaacattaa
     8041 agaagagaac agcccattta tagtgtttaa ctgtgcagac tatgcagaaa atcctaatct
     8101 attgatagct caactctttg gggtagtaaa aggtgcatat acaggagcag atgcaaatag
     8161 agaaggtctt gtagaacaag ctaataatgg aatattgttt ttagatgaaa ttcatagatt
     8221 gcctgctgct gggcaagaaa tcttattttc attaatagat agaggagaat ttagaaggtt
     8281 aggagaaagt agttctaata gaaaggcaaa tgttctcata ataagtgcta caacagaaaa
     8341 tccagattct aatttacttc aaacatttag aagacgtata ccaatggtaa taaatttacc
     8401 taatatgagt gaaagaccaa aatctgaaag atatgaaata ataataaagt tttttgaaag
     8461 agaagcaaag cgagtaaaca agaattttat aataactaaa gaagttatgt gtgcacttat
     8521 gaattataag tgtactggaa atataggtca gttaaagagt gatatacaag taacttgtgc
     8581 tagagctttt agtaaatcga tattttctag cgataaagta attatagatt tagatagttt
     8641 aagggattat ataaaaagtg gatattatga ttgtaattgt aaaaatgaaa agtattctga
     8701 gttttcagtt gaggatatat atatagatat tagtgaattg gtgagtggaa aaaatagaat
     8761 tttatctgat aatgagataa gcgagatata taattatgca gaaaaagagc ttaaggtttt
     8821 ggaaagtaag tgcttttcag aagaagagtt aaaaaatgca tttatagaaa agttagataa
     8881 gaagtttaat gctattaaaa acaataaaaa tttatctgat agaagaagtc gaatagactg
     8941 gggaggacaa ttaaaagagt ctacactgga gataatggat aaggtaattg tatttattaa
     9001 ggagcagttt aagcatgtaa atcaaggtct gtacttggct ctagcaattc atattgagca
     9061 tgctataggt aggataaaag atggaaaaac tataataaat ccgagtttag ataaaataaa
     9121 agcatcaatg ccaatggaat atgagttgtc aagatatata atgggtatag tagaagattt
     9181 gacacaagta acatttccag aagatgagct tggatatttg gcttattata taaataaatt
     9241 ttgttataat gaggaaagta taaaagataa agttaaagta gtaatagtta cgcatggtaa
     9301 agttggaatt gaaatgtcta aagttgtaaa tcatatttta ggtatagaat gtacactagg
     9361 tatagaaata gcacttacag attcaccatc agaggggata gagcatgtat tagaagaact
     9421 tcaaaagata gaagctagaa aaggtattct agttctaatt gatatgggtt ctttggtaat
     9481 tcttggtgat gaggttgaaa aaaggcttgg aattagatgt aaaactgtaa atagagtaga
     9541 tacattattg gcaatggaag caggaaaact tgcaacaatt gaaggaaaaa gtttagatgg
     9601 tataatagct gatttaaaga aaaataagaa ttatgctatg ataaatacaa ataaatttag
     9661 ttacagaaaa aacgagtatg gaaagaaaaa tgtaatcatt actttatgtt tgagtggtgt
     9721 aggtacagct ttaaatttaa aagagcatat agaaaaacaa attaaggaat atgacacttt
     9781 aattgaagta aagccagttg cttttttaaa taataattta gaagaagagt taaatgatat
     9841 tgaatctgaa tataatatta ttgctatctg tggaactatt gatatagatt ataaaaacgt
     9901 gccttttatt tcttataatg aggtacttgg taaaaatggt attaataaag tattagagca
     9961 tttaaatagt aaacaaccag ctttaaatat tgaaaaccag ttgaataatt taatacatga
    10021 agatttgatt ttatacaatt ttgaaggaat ttcaaaggag tatataattg atactcttgt
    10081 ttcaaaactt gaagaaggtg gatatgttga ctcaaagtat atattaagtg tatataaaag
    10141 agaagcaatg ggaagtgttg taatggcctc taaggtagcc gtgccacatg gacttccaga
    10201 aaacgttatg aagcctgcaa tagcaatcgc gaggttgaat aaacctatag tatgggacaa
    10261 taaatttatg gtggatttgg tggtactact ggcacttaaa gaaaataata agaaagaaat
    10321 acgaagctta ttttcaaaga ttaatgacca gcatacttta gaagttttat taaatactga
    10381 agataaaaat gaaataaaag aacttttaag ctaattatta gtaccgagaa gaaagacaaa
    10441 aaaatttgct ttagtataaa atttaaaact actcaattgc taagtagtta attactctat
    10501 ataaggctag ttctatgaaa tttatttata tgattttata gaattagctt ttttcttggt
    10561 aaataaaata tataaattta tttatgtaaa aagtaaattt tattggacaa ttctataaaa
    10621 atttttccta tatttcttat aaaatataga atatataaaa aatatataaa aataaattaa
    10681 attcataata aataatttta taaaatagtt gttaaacata aaaaaccatt tgtaaaaaac
    10741 aaaaaaatca tttctctaac aaaaaaaacc acaaaaaata taaaaaagta tggctagtta
    10801 agtgattaac aagtaaaaat taaaataaaa atcaaaaaaa atttatcaat gaaagtcagc
    10861 taaaatcctg gaagagagca ttatttttgg gaaaacattt tcttttgttt cacaaaaaaa
    10921 gccacttttt ggcacacaac atgctttata taaagtcaag aaaaactaag gtataagaca
    10981 aaggcaatta ggaggtgtaa atatggagtt tataaatttt gtaagtgata aagttataga
    11041 ccttaaaatg gattgtaaaa cgaaagaaga tttctttaaa gaaatacata ataaagtttt
    11101 tgatttaggc tttgtaaaag aagaatttgg agaaaaaata ttagcaagag aaaatgtatt
    11161 tccaacaggt ttaaatttag gtgattatgg agtagcaata ccacatacag atgcggaata
    11221 tattaaagag caatttatat cagtctgtac ttttaatgaa ccagtagtat ttagttctat
    11281 ggaagaccaa gatgaaaaag tacctgtaaa tttggctttc gtattaggac ttaatcaacc
    11341 acacagtcaa ttatctgtat taacagaatt aatgggaatt atgcaaaata aagagttagt
    11401 tgataagttg atgagttcta ctgataaaga agaagtttta aagacactaa agtctttata
    11461 aaaataaaaa agtactaaaa ataaaaaata taaaaataaa aattattagg aggaaataaa
    11521 aatgaaaaaa atattagtag catgtggagc aggaatagca acatcaacag tagtttgtga
    11581 caaggtagag agattagtta aggaaaataa tatacaagca gaagttatac aatgcaaaat
    11641 agctgaatgt gcaacaaagc aagaaggagc agatttaata gtatcaacaa ctatactacc
    11701 aacaacttat aatataccag caataaaagc aacagcatat ataactggag taaatacagc
    11761 agcattagat aaaaaaatat tagatgcttt aaaataatcc tatcatagat acattgaaat
    11821 aatattttat aaaacagaaa aaggaggatt tataaatgga taaattatta gcagtagtac
    11881 aatacatttt aaatatggga ccgacagtaa tactaccaat aacaattgta gtactaggaa
    11941 tgatatttag aattaagttt acaaaagctt taaagtcagg tcttacaatt ggtataggat
    12001 ttgtgggtat aaacttagtt gtaaacctat taacatcaac actagggcca gcggctcaag
    12061 gaatggttga aagattcgga cttaatttaa cagtaataga tgttggttgg ccaacaacag
    12121 catcagcaac atgggcttca ccagttgcac caatattaat accagtttgt ttattagtaa
    12181 acttactatt attatacttc aataaaacaa agatattaaa cgttgatata tggaattatt
    12241 ggcactttat agtagcaggt gcagttggat atatagtaac aggaagttat gtttgggcta
    12301 tattctgtgc aatactaatg gagttattag tactgtttat tgcagaaaag acagcaaaaa
    12361 atgttcagga gttttatgat ttagaaggta tagcattacc aactggttca acagcttcat
    12421 ttgctccact tggatattta gtaggtaaat ttgttgaaaa aataccagga ataaataaaa
    12481 tacaagcaga cccagactct atacaaaaaa gatttggtat atttggagaa ccaatgatga
    12541 tggggattat actaggtata atacttggag cacttgcggg atatgatgta gcaggaatat
    12601 tcaatgtagg tatatcaatg ggtgcagtaa tgttacttat gcctagaatg gttagaatat
    12661 taatggaagg tttaattcca atttcagaat caataagaga tttcttacaa gcaaaatatg
    12721 gagacagaga actttatata ggtctggatg cagcagtagc aactggacat ccagcagtta
    12781 tatcaacagc tcttatactt gttcctataa ctttgttctt agcagttata ctaccaggaa
    12841 acaaagtttt accatttgga gacttagcga caataccatt ttatatggca tttatagtgt
    12901 gttttagaaa aggtaatata gttcaatcag ttataacagg aacaatagta atagctatat
    12961 cactttatat ggctacaaac tttgcagatg ttcatacaca attattacaa caagcaaata
    13021 tgatgccaga aggaacaaca atggttacaa gtatagatac aggtggaaac ttgctaaaat
    13081 ggggaatatt gaaattatct caattagttt cttcatttgg aatattctag tatgtagtta
    13141 atagagatta ctctttttta gtaaagtatg cgaaaattta acatttagca gctactaaaa
    13201 aagagtaata ttttttataa atttttaaaa atacaaaata caagaggagg agatttatcg
    13261 tgaaagcagc agtattacat ggcacaaatg atatgagatt tgaagatata gaaataaaac
    13321 catgtgaaag tgatgaagta aaaataaaag ttatggcagc aggaatatgt gggtcagatc
    13381 ctccaagagt attaaaacat tggaaatatc cagttccagc tataccagga catgagtttt
    13441 ctggagtaat tgctgaagtt ggtaaggatg taaaaaatgt aaaagttgga gatagagtag
    13501 ttgcaattcc ttttatacct tgtaatgaat gtgaatattg taaaagagga ttattttcat
    13561 tgtgtgatga ccatggaatg ttaggagcta aaagttttgg agcttttgca gagtatgtaa
    13621 atataaaagc tacaaatgta cttccaatag gagatatgaa ctttgaagat gctgcaatga
    13681 tagagccact agcagttgct atgcatggag tacttaatat aggtgttcaa gttggagaca
    13741 ctgtagctgt aatgggtagt ggaacaatgg ggcagttggt aatacaagga cttaaaatag
    13801 caggggcagg aacaattata gcagttgata tatctgataa taaattaaga gaatcaaagg
    13861 aattaggggc tgatataata attaatgcta aagatattaa cccagtagaa aaaataaaag
    13921 aacttactgg tggaaaaggt gttgacatag ctcttgaatg tgcaggttca aaaataactc
    13981 aagaacaatg tttacttata actaaaaaga aaagtaagat aggattttta gggatagcat
    14041 attcagatat aactttatca gaagaagcat ttgaaaatat atttagaaaa gaactagaat
    14101 taaaaggttt ttggaactct tattctgcac catttccagg acaagaatgg actaagggaa
    14161 tcaatctagt taatgaagga aagataaaat taaaagaaat ggtatctcat agattctcac
    14221 ttgaagatac ttacaaagca tttgaaatga taagagatag aaaagaagaa tttaacaaaa
    14281 tattaatttt accacaaggg gttgaaaaat aatgaaagca gtagttaaaa ctaaaccagg
    14341 ctatgataat gtagaagttt tagaagtaga agaaccaaaa gctacaggag acaaggttaa
    14401 aataaaagta gaatatagtg gtatatgtgg ttcagatata cattcattta aaggtgaata
    14461 tgcaaatata aaagccccag taacattagg tcatgaattt tctggaattg ttgtagaagt
    14521 aggagaagat gttaaaaata taaaagtagg agatagagtt acatcagaga ctacttttgc
    14581 aacttgtgag aaatgtatat attgtgaaac taaagattat aacttatgcc caactagaaa
    14641 aggtattggt actcaagtaa atggaagttt tgctgagtat gtattatcaa gagaagaaag
    14701 catacatgta cttcctgaaa atgtatcttt attatcagca gcccttacag agccactagc
    14761 ttgttgtgtt catgcagctc ttgaaaagac tactatagaa agttctgata cagttttaat
    14821 aataggacca ggaccaattg gtttattatt agctcaagtt gttaaatctc aaggtgctac
    14881 agttataatg tctggtgtta caaaagataa ggaaagatta gaaatagcta aaaatcttgg
    14941 agtagataga actgtaaatg taatggaaga aagtttggca gatgtagtga atgaaatgac
    15001 tgatggaaat ggagtaaaca aaggatttga ttgttctggg tttatgccag cagttaacga
    15061 agccttaaga ctgcttagaa aaaaaggtac tttcgtacaa gttggaatct ttgctaaaaa
    15121 attaaatgaa atggaccaag aagcaataat ccaaagagag ttacaatata taggaagcag
    15181 gtctcaaaag ccaagttctt ggattacagc attagattta atggcaaatg ggaaagtaaa
    15241 tacagaagct ttagttacta gaactgttgg tttagacgaa tttaaagaag gtattacagc
    15301 tttaatgaac ggggaagaaa taaaagtagc agtaaaatct taaaaaaaca tttgtattat
    15361 tttaaaaaaa attgtaaaat ctactgtagg ataaaaaaat gtaaggtctt aaaaaataac
    15421 aggaggaaga aagtatgagc actcttagag atcattctaa taatattaga aaaaacataa
    15481 taaaaatggt tgcagaagct aaaagcggac atccaggtgg ttcattatca atagctgata
    15541 tgttgacagt attatatttt gataagatga acgtatgtgc agaaaatcca aaaatggctg
    15601 atagagatag atttgttcta tcaaaaggtc atgcagcacc agctttatat gctacattag
    15661 cagaaaaagg atttttccca gaagaagaat taataacact aagaaaattt ggttctaagt
    15721 tacaaggtca cccagacatg aaaaaagtag caggaatcga tatgtctact ggttctcttg
    15781 gtcaagggct atctgcagca aatggtatgg cacttgcagg aaaattagat aacaaagact
    15841 atacagtata tactatatta ggtgatggag aaatccaaga aggtcaaata tgggaagctg
    15901 caatgacagc atcacattac aaattagata atttaattgc atttgttgac ttaaatggat
    15961 tacaaataga tggttctaat gaagaagtaa tgaatgtaag tccagtagat gacaaattca
    16021 aatcttttgg atggaatgta atagtaataa atggacattc ttttgaagaa ataggaaatg
    16081 cagttgatga agctaaaaaa gttacaggaa aaccaactgt aataatagct aaaacagtaa
    16141 aaggtaaagg tgtttcattt atggaaaata atgctgcatg gcatggaaca gctccaaatg
    16201 cagaacaagc agagcaagca ttgaaagatt tagaaggagg tctataggaa tgggaatagc
    16261 aactagagaa gcttacggac aagtgttaaa agaacttgca gaaaataaag atatagtagt
    16321 attagatgca gatttaggaa aagctacaaa aagtatatca tttaaagaag ttgccccaga
    16381 tagatttttt gacatgggga tagcagaagg tgacatgatt ggtacagcag caggtcttgc
    16441 tacttgtgga aaaatacctt ttgcgagtac ttttgcaata tttgcagcag gaagaggcta
    16501 tgagcaaata agaaactctg tagcatatcc aaatttaaat gtaaaaatag cagctactca
    16561 tgcaggtgta acagtaggtg aagatggtgg tagccatcaa gctatagagg atatatctct
    16621 aatgagaggt ataccaaata tggttgtatt aaatccagca gatgctttag aagcaagaca
    16681 agctattctt acatctattg attataatgg acctgtttat ataagattag gtagagctgc
    16741 aactcctgat gtaaacagtg aaaattataa atttgaaata ggaaaaggaa ctgttttaag
    16801 agaaggttct gacataacag taatagctac aggaataatg gtagcaaaag ccttagaagc
    16861 agcagaagaa cttgctaaag aaggtataaa tgtagaagtt gttaatatat ctacaataaa
    16921 accattagat gaagcattaa taaaagaaac tgctaagaaa actggaaaag tagttacagc
    16981 agaggagcac agtataatag gtggtttagg ttcagctgta tgtgaagctt tagcagaaac
    17041 taaagatgta gtagttagaa gaattggtgt taaagatgta tttggtcaat caggtacacc
    17101 agctgattta ttaaaacact atggtttaac tacagaagat atagtaaaaa atataaaaga
    17161 gttgttatag atataaatat atctaaataa caaattgatt tgacataagt caattaaagc
    17221 tgtcttaaaa atagaaagaa atttctacaa tgggacagct ttatttttac aatactttat
    17281 ttttatgata attataaaat agtctgaaag taatcaaaaa aattataaaa agaggtgcta
    17341 tttaatgaaa atatctattg gatgtgacca tggtggttat gaattaaagg aatatataaa
    17401 ggaaaattta ataaataaag gtatagaagt tgaagatgtg ggtacaaatt caaaagaaag
    17461 tgtagatttt ccaatatatg caaaaaaggt aagtcaaaat gttcaaagta aaaaatctga
    17521 tttaggcata ctttgttgtg gaacaggaat aggtatgtct attgcagcaa ataagtttaa
    17581 aggaatcaga gcagctgttg tttctgactg tttttcagct aaggcaacta gagaacacaa
    17641 taatacaaat gtactttgtt taggagaaag agttattggg aaaggattag caatgagaat
    17701 tgttgaagaa tggttaaatt caagttataa tggagaaaga catgcaagaa gattacaaat
    17761 gattgatgat atagaaaaag aaaatttata aatatataat ttataacaag ggggagcgtt
    17821 atgtctatta tttgtgcttc aataatgtgt gcagaccaaa tgaagttaaa agaggaatta
    17881 gaagctcttg aagaagcagg tgtaaaatta cttcattgtg atgttatgga tggaatattt
    17941 gttaaaaact tggctatggg accagagtta ttaaaatcaa taagtgaaaa tacaacaata
    18001 cctttagaca tacacttagc cacagaaact ccagataaat atattgatat gatgtcttat
    18061 ataaagccaa aatatatttc ttttcatgta gaatcaagta caaatgtaaa agctgatata
    18121 caaaagttaa gaaattatgg tatagggcca gtgttagcaa taagccctca aacaagtgtg
    18181 gataaaatag aagaatatat aagtttagtt gagggtatct tagtaatgac agtaaatcca
    18241 gggtttgcag gtcaaaagtt taatctttct gtattggata agttggataa acttacagag
    18301 atactaaaag attatgataa tcctccattt atagaggttg atggaaatat aaataaggat
    18361 actattaaac ttatgaatgg taaaaaagta gatatatatg ttgttggtac ttcagcttta
    18421 tttaatgata agcctccaat ttcatataaa gataaaatag aagaactaaa agaaagtata
    18481 aagtaaccat gtaataaaat tataatatgt ttctgtttaa tagtaaaaat ttaggtggaa
    18541 acaagaaatt ttataatatt taataaaaat tttggttgag taaggagaat tttttatggg
    18601 agacctatat aaactacaaa atggtactga tattagagga atagcgtatg aaaatgattc
    18661 taaagaagtt aacctaactg tagaagaagt aaaaaaaata gctaaagcgt ttcatatttg
    18721 gttaaaagaa aagactaaga aagataaagt aactgttgcc ataggaactg attctaggat
    18781 aacaggaagc caatttagaa gtactgttat tgaaacttta actaatgacg attgtaatgt
    18841 aattgattgt gagattgcca caactccagc tatgtttatg acaactatta tggatggata
    18901 caattctgat ggggcgatta tgattacagc aagtcactta ccttattact ataatggttt
    18961 aaagtttttc acagaaagtg gaggtttaga aaaaactgat ataaaagaga tgttggatat
    19021 agctgttaaa aatgatagtg tagactatga ggaagtaaat aaaaaaggtg aagttatagt
    19081 aaaaaattta atagaagatt attctaattt attgatagat aaaattagaa aaggtgtaaa
    19141 ttcaagtaaa aattatgaaa agcctttttc tgggcttaag atattggttg atgcaggaaa
    19201 tggggctggt ggattctttg ctgaaaaagt tttacacata ctgggagcag atacgacagg
    19261 tagtcagttt ttgaacccag atggtatgtt cccaaatcat atacctaatc ccgaaaacaa
    19321 agaagcaatg gaaagcatat gtaaagctgt gttagataat aagagcgacc taggaataat
    19381 ctttgataca gatgtagaca gagctgctat tgtaggtaaa aatggcaaac caataaataa
    19441 aaatgctctt atagctgtta tatcatcaat agtattagaa gaacatccaa aaacagctat
    19501 agtaacagat tcgattactt ctgaaggact tgctaaattt ataaatgaat tacaaggaag
    19561 acatcataga tttaaaagag gatataaaaa tgtaataaat gaagctataa gattgaatag
    19621 tgaaggtgaa gagtgccatt tagctataga aacgtctgga catgctgctt taaaagaaaa
    19681 ttatttctta gatgatgggg catatttaat agctaaaatt ttaataaaag tagcaaagct
    19741 atccttggaa ggaaagacta tagaagaatt gatagaaaac cttgaagaag ctcaagaaga
    19801 gaaagaagtt agaataggca ttaataaaga agattttaga ccatatgcag aggatatatt
    19861 aaaagattta gattctcatg tcaagagtat tgatggatgg tctgtagcac caaataattt
    19921 tgaaggcata agagttaatt gtgatgaatc aaatggagat ggatggtttt tacttagaat
    19981 atcactacat gaaccattat tagctttaaa tattgaatca aataaaattg gcggagcaga
    20041 acaaatttat tctaagttaa aacttttctt agaaaaatat gatttaaaag gtctttaggg
    20101 taaatagaaa gaatatattt atattgaaga ataaatgtaa atttgtacct aagattacaa
    20161 atattacaaa attgtaatct taggtaacag agatgtaact gattatggaa acactatgtg
    20221 atattataga tacagagaga tgaaatatgt gatgaattat agagaaatat aatttaaaat
    20281 gtattaatat cacaccaaaa aggtataatc aaaaaatatg taaatataaa ccccaacatt
    20341 tagaagaaga cctctaagga ggtttttttc ttgtttaaat aacttaaaat atatttttat
    20401 atcaatatat caattaactc ttctaaaagt aagcattacc ttaaataaat cagcatctat
    20461 aattatttcc atgtgtgcat tttctaattc aattatattt tgagcaattg ataaaccaag
    20521 accagagcca tctgtttttc tagatatatc accccttcta aatctttcta ttaactcttc
    20581 tggctttaga tttagtttat catttgaaat atttttaaac gttaatatta tattttcttc
    20641 ctttataaac atatctatgt agaccctagt gttaggcata gagtatttta gtatattgca
    20701 aattagattc tcaaaaactc tccaagtttt tttaccatct atcatggtaa aaacagcata
    20761 ttcaggtgtt tctattataa aatctaaact gctattttga attttgtctt caaactctac
    20821 aatactttga attaaaagtt ctcttatatc ggtttttgtt atattaaatt ctacatctcc
    20881 actacttagc ttagatgctt caaatatgtc atctattaat aattttaatc tttgtgattt
    20941 tttatctaaa atatttatat agtcatttat agtctctctg tcaaagtcat tattttttaa
    21001 catatcaaca tagcttaata tagatgtaag tggagttttt aagtcatgag atacgtttgt
    21061 gataagctct gccttcatcc tttcattttt tatattagca tctacagcag tttcaagacc
    21121 acctgttata ttatttatac ttttggctaa agtagatatt tcatcatctc ctataacttc
    21181 taatttatac tttaaattgc cattttctat attttttata ttttttgata tgttatttat
    21241 gtaattaaac ttaacagata aaaagttgta tatataaaaa tgaaaaactg tgtttacaag
    21301 tgtaaataaa attactatag ctattgcctc agcataatat atatacataa agaatatcaa
    21361 aacaagatat tggataatag cttgtacaac tatagatatt aaaaatacta tagctaattt
    21421 tttatttata gtttttttat tatacatatt aagcaagttt gaaaaaaata catatgttat
    21481 agacatatct tttagattat agttattata tttaatattt tcatatatta tatagactat
    21541 agatataatt ataaaattgt agattattaa attagtgttg taaaacattt tattacttgt
    21601 aaatgcatga aaccttaagg ttaatagtat caaagttaat ataagtgtaa gttctatttt
    21661 taccttagaa aaaaggctta tgacattctc taagaatttg actttagtct gtttattttt
    21721 tttatacaat gtaaaacata gtatagtcaa taaaaaactt gataatataa tacctatgca
    21781 gacaaactct aattctactt ttgacaaatg tacaggagta ccgaaaaaat aaaaataagg
    21841 gagagcaaac tctttataat aataattaat attataagaa aagttattat tcaaatcata
    21901 aactgcttca aataatatag aaactaatag aaacagtaat aataataaaa taaattttgc
    21961 tttagaatca ttaataatta agtttttcaa ttttgtaacc aatgccccac accaccttta
    22021 aatattttgg ttcttttgga ttaatttcaa tcttttctct aattcttcta atatgaacag
    22081 caatagttct ctcacaacta taagcatcct cattccagac attttcataa atctcatgag
    22141 aagagaaaat tttacctttt cgtgacataa gaagtgtaag tattttatat tcaagtggtg
    22201 tcattttaat ttcttcacca tctaaagaaa ctatttttgt atctgtattt agctctaggc
    22261 caccactttt tagaatataa ttagtatcat ttgagttatt tgtattacta aaatcaccta
    22321 aatttatata ccttctaagt tgagatttta ctcgagcagt aagttctagt ggattaaatg
    22381 gtttagttat atagtcatca gcacccatat taagacctat aattttgtcc atatcttctg
    22441 attttgcaga taagagaatt attggtatat tccttttttc tcttatttta agtgtagttt
    22501 taagcccatc taaattgggc atcattacat ccattattat taacttaata tcattttcga
    22561 ttagtatgtc caatgcttca agtccatcat atgcctttaa aacattaaag ttatcaggtc
    22621 tcaaatagaa ctctatagca tcaactatct ctctatcatc atctacaacc aatatatttt
    22681 gattcaactt attaaccccc ttgaaatttt atttttaacc aatttttatt ttataccaaa
    22741 aagaggtatt agaaaacagt aagcccgttc attatgaaat aaaaactagt acttttaata
    22801 tattgtcaat gcatatagta aatttaaaag tatagacgcc catattaatc tgaaggtaaa
    22861 agtttatgaa aatatctagg gtaatctatc acactcctta tgttattttg gtttattaat
    22921 tttttaggtt taaacatata ttaacaaata taaataacaa atgagtttca taattattaa
    22981 gaatttataa caaatattta ttttaatgta gtttaatata aataggtagc ttaatgtaga
    23041 tatgtataaa gcatttaata aggcttatag gttttaatga tgatatcttt atatatttta
    23101 agagatgatg aaattagata tgggtaatag ttgtatgaga aaaagtagta tagcacaaaa
    23161 agtgctatat cacaaaatct caaataattt cctttttata acttaaatta aaagaataaa
    23221 tatttcttat aaattaggtg tatgaagtat agcaggaaca tctttattat ttaataactt
    23281 atcaaatgat ttatggtcag acttatagcc aataccttct ctatattcta attcatcaac
    23341 aattttgtat tgttgacagg catttttaac cttttcatag tcttcagtag aatgagcttt
    23401 ataaactatc ttattattaa aagttatttt atcagcaaat ttaggtttta caaaatattt
    23461 agcatctttt attttaccag cattatataa attttggata acagaaccat tttttaaatg
    23521 ttgtaaatgg tcatctaact tataagtatg tgctatatga gttacatttt taaatttttc
    23581 ttcaaatgta agagcaattt tttccatgta attgaagtca gtattaccat ttgtatttgt
    23641 ttctggtaat aatattatat tttcccttat tacaccaagg ctattaagag aagctaaaaa
    23701 ttctctattt ctatattctg atttttgtat atttgttagg ttttcatatt ttttatattt
    23761 atcaaagacg gatataccac atccagtaga tactaaaacc acaaatacat gattttttcc
    23821 tctttctttt atagcattaa caatagcact cccagcccac aacacttcat catcttgatg
    23881 ctgaacatag aagacaacat aatctttaaa ttttttatga ctataattca catagctact
    23941 aaaagcggtt aaaatcaaac ataaaactag aataatagat agaattttat atttatttat
    24001 agtaaatttt ttattcttaa atgtcattta atcatctcct gaaaagttgt cttagttata
    24061 ttatcaaaca aagtactaaa aattatttct taaatgctga aaaatataga gtagattaat
    24121 ggaataaaat ttttttatta gtttaaaata aagaaaacat ttatgtataa gactaatcaa
    24181 aatattaatg attgatagga attgagattt aaagggattg tacatttatt cgggattcat
    24241 tttaatagaa ttttacttta gtacttagaa taaaagtaat aactttttaa atttaaagag
    24301 tacacatata tatttgtgta ctccttgatg atatgattgc ttatttacta caaaaacaga
    24361 gtctttttaa acctaaaata tttgatgcac cagctatcgt agttccataa tagtaaactg
    24421 gtttgttaag tgatagaaaa tttataattg agttattaca taatgtactt cctgtgcaaa
    24481 tcactaaatc agcccaagat attacatctt cataatctcg tataccatct tcaatcttaa
    24541 cattatattg gatagtatcc acaaattcag gatttaaatc taatactctt gtttcgaaaa
    24601 agtccttaat attatcaatt atggctggtt gatatcctat aattgctact tttggattac
    24661 caaattccat ttttataaac tcagggaatt tttttgcaca tatctctggc tcgttatttt
    24721 tacaatgaat ggttctatcc gtcttgccta agtatttcat aactgcattt aatgatgcta
    24781 taaaaagact tctatcatga ggattgtcat gtaaatctaa tgataatatg tcttttaaag
    24841 aaccttcaaa tgtagatgga gtagaagtaa atgcttgtcc tttagctcct ttaaagtcag
    24901 cttcaagcat tatttcttta ccaactataa ttgggaaatc tttacgctta gtatttccaa
    24961 ttgcttcttt tgaagataga gatttggaca caatacttat catatccata tttaaattat
    25021 atttatcaac ttgtactaat agataatctc tcaaaatatc aaatatattc atagaaaaaa
    25081 gattctcctt tattttttat ttcttcattt aaatcaaaaa tatgatttat ttttccattg
    25141 ttcattattc ctatcttatg agcaaaaaac attgcttcag aaatatcatg agtaacatga
    25201 atcactgttg gtttgtattt tatgtagaca tccttcaaaa ttttgcttat atgttttttt
    25261 gtaatataat ccaatgaact cataggttca tctaaaagta atatatcagg ttcagctatt
    25321 aaggcacgtg caaaagcaac tcgttgcttt tcacctccac ttaaattttt aggataccta
    25381 ttttttatat cttctatctg cattaattta gataagtggt tagccttaag aatctgttca
    25441 tctataggta tttttctcaa tttaagtgga aaccttatat tatcttcaac attcatattt
    25501 ggaaataatt caaaattttg atatacaaat cctatatttc tattttctgg aggtaagttt
    25561 gatattaaat tttctttgta tgatacattt cctttagcag atggatatct tcctgctaat
    25621 gtttctagaa atagagtttt acctgaccca ctagctccta ataaaacaaa gtattcatga
    25681 ttattaatat ttaaactatt ttcttgtaat ttaaaattag gtaaatcaag agataaattt
    25741 tttattttta acatattaat tcttatcaac ctctctatta aaaaatatct caaaaagcat
    25801 caagcatgta agtgagataa atatcaaaat tacagctatt ccgattgcaa tgtctatatc
    25861 accagtagac atatttaaat atatagatgt aggaataatt tcagttttca tacgtgtaga
    25921 acctgctatc atagctactg caccaaattc accaagtgct cttgaccaag tcattacagt
    25981 tgcagatatg attccagttt ttagatttgg aagaattata aatctaaatg cttcccaact
    26041 attacatcca agggtacgtg caacaaatat catcttttca ttactttcat ttagtgaagt
    26101 gtaaagtatt ttaatagtaa gtggaagatt tacaaatact tgtgctaaaa ctataccttg
    26161 ctttgtaaat ataaaatcaa gcttaaaaat cttataaatg gaatctccaa tccccattct
    26221 tccaaacaaa agtaaaagag caattcctga tactaagtgt ggaagagaca tagggagatt
    26281 tattatctgt ataatcagtt ttttaaatgg taatttagta atatgtaaaa aatatgctac
    26341 tggaatagct aataaaaggc atactatagt agatattaaa gatgttttaa tactaagtat
    26401 tattgcatac tgtacttcca ctgattttaa gcatatagga acataactta tccctttaat
    26461 gattaacata agaagtggtg atacaataaa caaaaagact atagcaaaaa atccagtaag
    26521 tagagtagta aaaaatttat ttttcaagtt taatcactac ttaacaggtt caaaaccata
    26581 ttttttaaat atctgtttag cttcgtctga ctctatataa tttataaatt tattagctaa
    26641 atctttttta tcactatagt ttacgattcc tgctgatata agttggtcta tatttttatc
    26701 ttgggcaatt tctataactt ctacatcttc attgccaaag accgaatctt ttgtggctat
    26761 tgtagcatca gctttacttg atgttaactg tacaatcatt tcatttacag ttgcagctgt
    26821 agagacaaca ttatcattta tagattttaa attatttttt tcaagtattt tttgtgtagt
    26881 cttacctata gcattagact ctttgtctcc caaaataagt ttaactccat ctttatttaa
    26941 gtcttccaat ttttgaatat tagctggatt tcctttaggt acaactattg caggtgtatg
    27001 atgtgcaacc gttttatgtt cacctacaag ttctttttca aaggcttttg aataaattgg
    27061 ttcagaacca acaataaata cgtccccttt gtgacttgtc tcaatttgag ctattagctg
    27121 tgcagaacct gcatagctat attcaacttt gacattattt tctttctcaa atttttttgc
    27181 tatttcatcc attggtttct ttaaacctgc ccctgaatag acaaaaagag tctctttttc
    27241 ttttgaatca tcagctgttg tcactttagt attagattca tcattagctt tgttttcttt
    27301 agaattacat gcagtaatac ttaatgaaat aatcatagct aaagctagta ctgaaagttt
    27361 tttaaacata agatattccc ccttagtaaa ttaatgtaaa ttctttttta gtatatcata
    27421 ctaagaaaac gatgtctaag tttttgttat gttttataat gttttgttat gttttgttat
    27481 gtatagttaa gaaaaaatct atttataatg ttatttctat aggtagttat tttattttta
    27541 atttttatta atactttaaa gtattttaaa tttaaaaact taaatcaaat taacttattg
    27601 tagatttttt actttttaac taattttata tttattatat
//
LOCUS       DAYAOY010000050        27097 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYAOY010000050 DAYAOY010000000
VERSION     DAYAOY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.050"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            108..848
                     /locus_tag="ACGI6B_002236"
     CDS             108..848
                     /locus_tag="ACGI6B_002236"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HFG9751678.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            860..2638
                     /locus_tag="ACGI6B_002237"
     CDS             860..2638
                     /locus_tag="ACGI6B_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751679.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            2805..3722
                     /gene="pfkB"
                     /locus_tag="ACGI6B_002238"
     CDS             2805..3722
                     /gene="pfkB"
                     /locus_tag="ACGI6B_002238"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFG9751680.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            3759..5678
                     /locus_tag="ACGI6B_002239"
     CDS             3759..5678
                     /locus_tag="ACGI6B_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9751681.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            6274..7800
                     /locus_tag="ACGI6B_002240"
     CDS             6274..7800
                     /locus_tag="ACGI6B_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9751682.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            7828..8385
                     /locus_tag="ACGI6B_002241"
     CDS             7828..8385
                     /locus_tag="ACGI6B_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751683.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            8907..9944
                     /locus_tag="ACGI6B_002242"
     CDS             8907..9944
                     /locus_tag="ACGI6B_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751684.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            10055..11197
                     /locus_tag="ACGI6B_002243"
     CDS             10055..11197
                     /locus_tag="ACGI6B_002243"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9751685.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            11651..13042
                     /locus_tag="ACGI6B_002244"
     CDS             11651..13042
                     /locus_tag="ACGI6B_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9751686.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            13295..15088
                     /locus_tag="ACGI6B_002245"
     CDS             13295..15088
                     /locus_tag="ACGI6B_002245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9751687.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(15169..16551)
                     /locus_tag="ACGI6B_002246"
     CDS             complement(15169..16551)
                     /locus_tag="ACGI6B_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9751688.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            16809..17156
                     /locus_tag="ACGI6B_002247"
     CDS             16809..17156
                     /locus_tag="ACGI6B_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9751689.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            17174..18208
                     /locus_tag="ACGI6B_002248"
     CDS             17174..18208
                     /locus_tag="ACGI6B_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HFG9751690.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(18257..19306)
                     /locus_tag="ACGI6B_002249"
     CDS             complement(18257..19306)
                     /locus_tag="ACGI6B_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9751691.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            19581..21116
                     /locus_tag="ACGI6B_002250"
     CDS             19581..21116
                     /locus_tag="ACGI6B_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751692.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(21301..21987)
                     /locus_tag="ACGI6B_002251"
     CDS             complement(21301..21987)
                     /locus_tag="ACGI6B_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFG9751693.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            22112..23494
                     /locus_tag="ACGI6B_002252"
     CDS             22112..23494
                     /locus_tag="ACGI6B_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9751694.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            23896..25164
                     /gene="eam"
                     /locus_tag="ACGI6B_002253"
     CDS             23896..25164
                     /gene="eam"
                     /locus_tag="ACGI6B_002253"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HFG9751695.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(25257..25613)
                     /locus_tag="ACGI6B_002254"
     CDS             complement(25257..25613)
                     /locus_tag="ACGI6B_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751696.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(25764..26858)
                     /locus_tag="ACGI6B_002255"
     CDS             complement(25764..26858)
                     /locus_tag="ACGI6B_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9751697.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT        10858 a         2919 c         4559 g         8761 t
ORIGIN      
        1 ataaccaaat actaaaaaat attttaaata aagaacttaa aattgtgata aaattaattt
       61 atagatatta aaaaaagtat aatatatggt ttttaagagg tgctaagatg aaaaatttta
      121 gagatttagg tgggaacaaa actgaggatg gcagaactgt aaagaaggga ttattttata
      181 ggtctgcaaa attatcaaat ctaagtgaaa atgatataaa gattttaaaa gagttaaata
      241 ttaaatatat atttgattat agaagtgatg aagaagctcg taaacatcca tcaacaataa
      301 tatcaaatat aaaaaatata agaataccag caatgagaga gctagaagaa tcaggaggaa
      361 gttttggttc tattgaagat atgattgatg gattgtttga aaaagatggt gcatttaata
      421 tgttaaataa tagttactat aatttgccta taaataatcc gtcatataaa aaattagtag
      481 aacttataag agactattct aatttaccaa tattaaatca ttgtacagct ggtaaggata
      541 gaactggtgt ggggagtgct ataattctta tgattttagg ggtatcgaga gagaatataa
      601 tgaaagatta tctaaaaagt aatgattttg cagataaaga gatagaaaga tttatagaat
      661 ataagcctaa atttaaggac attccaaaag aaaatttgaa atatatattt ggtgtaaatg
      721 aagagtatat gaaaactgca tttagaagaa ttgatgaaga atacattagt gtagaagcat
      781 atttatatgg agaatttaat ttaaataaag aagaaataag aaaattaaga aatcaatatc
      841 tagagtaaaa ggtgagttta tgagtttaaa tataacagca agacagataa atataataaa
      901 aatattgtta aattcaaaag atacaattag tggtattgcc ttaagtcaag agataggatg
      961 ttcttcaaaa actatacaaa atgaaataaa ggatattaat aaacaactta gaaatggtaa
     1021 aattttgtca ataagaggag ttggttataa actagaagga agttttgatg aggtaaatct
     1081 aaatagcaat ctctatgatg atgtagatag agtagaatat ataatcagaa aaatattaac
     1141 cttaagtaat gaagaaaaaa atactataaa acttgaagat ttagccgatt ctatgtatgt
     1201 aagtgtttca acagtaaaaa atgatttaaa agaagtaaaa agaatgttgg aaaagtattc
     1261 aatatcagtt aaatctaagc ataaacaagg tatatgtgtt ttggaaagtg aagataagat
     1321 attaaattgt atagttgact taagcaataa gagagataat caattgagtt taaatgattt
     1381 tttaaatgat gatataaagg aaagaaaact attaataaaa aaattattat tagacatatt
     1441 aaatgaaaat ggattagtat taactgatat agaatttaag agtgtgttaa ataatatatt
     1501 agttatgtta agcagacata agtataatga aaaagaattt atagagaatt atatagaaaa
     1561 ttataggagc aaaagaagct caattttaga aaatgaacaa aacaaagaaa ttataataaa
     1621 ttcaataaaa acattttgta aaaatttaaa attagctaca tcaatagata taagtaatga
     1681 taaggtattt gaagagtttt tgtataagca tattagtagt ttatgtaaaa aaataaagtt
     1741 aggtataaat caatcaccta taatggctca agatataaaa ataaaatatc catttgcttt
     1801 tgaactagct aatatagcta aaaagacaat agaaaaagat ttgaagatag aaattaatga
     1861 agatgaagtt gcaaacatag ctctacatat aggaggagca gttgaaagag catcttataa
     1921 taataaagat aaggttttca aaactataat agtttgtact tcaggcatag gcacatcaat
     1981 gttaataaaa gctaaattag agaatatatt taaggaaaaa ttagaaatac taaagattat
     2041 tccttcatac ttagttgaat atataaaagt tatagatgtt gattttgtaa tatctactgt
     2101 tcctattgaa gtaggagatg tgccagtaat aaatatatct ccaattttaa gtgagaaaga
     2161 aattagatta attgaaaaat atatagaaac aggaaatata tacctagatt tagatataaa
     2221 aagcctgttt gatagtgaat tgttttttac tgatttggtg tttaaaagga aagaagaagt
     2281 tatagatttt atggcaagta aacttgtaca aaagggctat atagatgagg atatgaaaaa
     2341 atcttacttt gagagagaaa aaatagcaac aacagaaata gggaatatgg taactatacc
     2401 acatggagct attggaaaaa tttttgataa taaaatagca attggtatat taaagaagcc
     2461 tatttcttgg gaaatatcag atgttcgttt aataataatg ttagcccttg ataaagataa
     2521 aatattagat tatgaagtaa tattttcaaa aatatataaa agagtagatt ctatagcaaa
     2581 agttataagt atatgtgaaa atagtagttt tgaaaaattt gtaaatttat tcaattagat
     2641 gggtagtatt gtatatacac ctgtaaattt ggataatata aaactaaata agataaaaac
     2701 gataaatttc tcttttaatt aagagaaatt ttttgacttt caaggatatt aatataaagt
     2761 taagatataa acataaagca aataaataaa aaagaggtgg caatatgatt tatacagtga
     2821 cattaaatcc atcaatagat tatatagtta agctagatga gctaaagaca ggaagtacaa
     2881 atcgtgtaaa agaagaatac gtttatccag gaggaaaagg tataaatgta tctcgcatat
     2941 ttaaggaact tggaaatgat aatacatctt taggttttat aagtggtttt actggagaat
     3001 atataataag aacattggaa gaaaaagaat tgaagacaga ttttataaag ataaaaagtg
     3061 gtttttcaag aataaatgta aaaataaagg cgttaaagga aactgagatt aatggacaag
     3121 gtccaaatat agatgatgaa gacattgata ctttatataa aaaacttgat aagctaaatc
     3181 aagatgatat tttaatattg gcaggaagta taccatctac tttagatgaa aaactatacg
     3241 aaaatataat ggctagatta gaaaaaaaga atataaaagt tgtagtagat gcaactaaaa
     3301 atcttcttct taatgtttta aaatataaac cttttctaat taagcctaat aacgacgaat
     3361 tagaagaact atttggtgtt aaattaaatt caattgaaga catggtaaaa tatgctagaa
     3421 ggttaaagga aatgggagct ataaatgtct tggtatcaat gggaaaagat ggagcattgt
     3481 tgattacaga ggaagatgaa gtgcttataa gtgatgttcc aaaaggaaaa gtaaaaaatt
     3541 ctgtaggagc aggtgattca atggtagcag gatttatatc tgggtatttg aatactggga
     3601 aatatgatta tgctcttaaa ttgggagcag cttcaggtag tgcaactgct ttttcatatg
     3661 acttagccaa aagagaatac attgataaat tggtaaatga gatatctgta aagcagtttt
     3721 aaaatattaa aaaatatata gaaaggtaat taatatttat gaaaattata gatttattag
     3781 atgaaaagtc aattaagtta aatttaaatt caaaaaccaa atcagaagca atagaagaat
     3841 tagtagattt ggtcgcaaat agtggaaatt taaatgataa agaaaactac aaaaaagcaa
     3901 ttttggctcg tgaagaaatg agtacaacag gaattggaga aggtgtagct atacctcatg
     3961 ctaaaaattc ttcagttact aaagcttgta tagcagcagc agtgtcaaaa gaaggtatag
     4021 attatgaatc gtttgacgga agtttatcaa atctattttt tatgatagct gcaccagacg
     4081 gagctaacaa tactcatctt gaagtattat caagattatc tacaatactt atggatgaag
     4141 actttagaaa taaacttata aattcaagct ctgaaaaaga atttaaggaa ataatagata
     4201 aaaaggaaag agaaaaattc agtgaagaat atcaagatga gaaagttact gaaaaaaata
     4261 taatagatac taaagagaat gatgctaata agtatcctaa ggttttagca gtaacagcat
     4321 gtccaactgg aatagcacat acatttatgg cagctgaaag tttaaataaa atggcagaaa
     4381 acaaaggtgt atcaataaaa gtagaaacta atggttcagc aggtgttaaa aataagctta
     4441 caaaagatga aatagaaaat gcaacatgta taatagtggc ggcagataaa aatgttgaga
     4501 tggctagatt taatggtaaa aaagtcataa aaactaaggt ggctgatggt atacacaaag
     4561 cggaagaact tatagacaag gctgtaaatg gagatgctcc aatttaccat ggaggagatg
     4621 gaagtcacaa cgaatcaaat gaagaaagtg aaagtggatt tagaaaagta tacaaacact
     4681 taatgaatgg tgtatctaat atgttaccat ttgttattgg tggagggata cttatagcga
     4741 tagccttttt acttgatgat tatactatta acccttcaaa ctttggttca aatacaccta
     4801 tagcagcatt ttttaaagga ataggagata aagcttttgg atttatgtta cctgtacttg
     4861 ctggatatat agcatactct atatctgata gaccagcgtt tgttgtagga tttgtaggag
     4921 gtgctctagc aggtgatggt ggttcaggtt ttttaggtgc actacttgca ggttttatag
     4981 caggttattt agtagaaggg cttaagaaaa tatttagtgt actaccagct tcattggaag
     5041 gaataaaacc agtattgtta taccctttac ttggaacact tttgatggga attataatga
     5101 catttttagt aatacctcca gttacagcaa ttaataatgc aatggttgga tttttaaatg
     5161 gtttaggtgg aacttcaaaa atagttttag gattagtatt aggtggaatg atggctgtgg
     5221 atatgggtgg acctgtaaat aaagcagcat atgtatttgg agttgcatca cttgaatcag
     5281 ggcagtttga aataatggca gcagtaatgg ctggtggtat ggttccgcca cttgctatag
     5341 ctcttgcaac tacatttttt aagaatagat ttactaaaga agaaagagat tctggtaaag
     5401 ttaattatgt aatgggatta tcatttgtta ctgaaggagc tatacctttt gcagctggag
     5461 acccacttca tgttatacca gcttgtgttg gaggttcagc agtggcagga gcacttagta
     5521 tgttgttcaa cgcagctctt cgtgcacctc atggaggagt atttgttata ccagtggtta
     5581 cccatccatt tgcatatata ttagctatag ctgtaggtgc tttggttgga atgatgcttt
     5641 tagcattact taaaaaacca ttaaatcaag aagtatagca attaaaggat attcaagaac
     5701 ttaaaatatg atgttttata aaaattaata tattgctgaa ttattttaaa ataaggattt
     5761 tatgaggctg ttatataacg atttgtaatt gttatcttga cagtctttta tttttatata
     5821 agtaaaataa gtgtgttttg ctttatttgt atttaaattg tttttaagtt agaatataaa
     5881 gaaaattata tattatttta atattcactc gaactagaaa ataatatttg ttgtattttt
     5941 ttgtatataa aatatattgt aatattaaaa aaataatagt aaactgattg cataaaaatg
     6001 atttttatat aaaatttaag cagaagaata aatttctaac gaaattggat atttcttctg
     6061 taaatacata ttcaaaaaaa gaattataag agtttagtca gcagtcgtgt aagtaataag
     6121 gactttataa atatatagaa atataatgat attaatataa ccaagcgatt tttaatttag
     6181 ctttttaata agcttatttt ttatactatt tataataaat aaactccatg atgggaaaac
     6241 tattactatt taatatgtaa aggagtattt taaatgaatg aatcatattg gtttttaaac
     6301 tcaagtccaa aagaatataa caaattaggt gaaaatataa aaacagattg tcttatagta
     6361 ggtggtggaa taacgggatt gacaactgca tatttattag caaaagaagg taaaaaagta
     6421 gtgcttgttg aggctgataa aataggatat ggaacttctg gaaggaatac tggtaaggta
     6481 acttgtcagc atgatatatt ttactctaag atagagaaaa aatatggatt agataaagca
     6541 aaatcatatt ataatgcaaa taatgaagct ttaaatttag ttgaacaaat aatagaagaa
     6601 aacaatataa aatgtgattt taaaagggaa acttcttttg tatttacaga aaaagaggat
     6661 actataaagg atattaaaga tgaatatcgt acatgtaaaa aaataggaat aaattgtgag
     6721 tatcatgaaa ctatagaaaa tattccatta gatataaagg gggctattag ttttactaat
     6781 caaggtcaat ttaacccaaa gaagtatatt gatggattgg caaaagcagc agtaaattta
     6841 gggctaaaaa tatatgaaaa tacaccagta gtagaccttg aaaaaggaaa aatatgtagg
     6901 gttaaaacaa gagaggataa tataatagaa gctgagaatg ttataatttc atctcatagt
     6961 ccatggtatg atggattgaa tttttatttt gcaaaagaat atgcagagcg tgcttattta
     7021 atggttgcag ttttggaaaa taaattagca gatggtatgt ttataagtat agatgaccca
     7081 tcaataactt ttagacaata taatgatgga agtgaaaatt tactgatttt tggaggagga
     7141 gaccataaag ttggtcaggg aggaactgag aaagaaatat ttgatgattt agagcattat
     7201 ggtaaagaag tctttaaggt aaaagatttt aaggggaaat ggtctgctca agataatatg
     7261 agttttgaca atgttcctta tattggttat atcaacaaaa gagaagacaa tatttatgta
     7321 gctacagggt tctctaaatg gggaattact aatggaactg ctgctggaat aataataaaa
     7381 gatttaataa taaataataa ttctgattat aaagatactt ttaatccatc cagactaggt
     7441 agctactttt caaaagattt tataaaggaa aatgcaaacg tagcaataaa ttatgtaagt
     7501 ggaaaattaa aaataggtag tggagatatg cctaaaaata atggagaagg aaagattgtt
     7561 aatattgatg gaaaaagata tggtgtatat aaggatgata atggagattt ttatattgta
     7621 gacactacat gtactcattt aggatgtgaa cttaatttta acagtgaaga aaaaacatgg
     7681 gactgtccat gtcatggttc tagatttgac tataagggaa atatacttga aggaccagct
     7741 cttaagccat taaaacttta tggacatggt gataatgatg ttaatcctaa gttattataa
     7801 aattattttt aggaaggtaa agtgtatatg aataaacttt taaagccaat aactatacta
     7861 ttgtatatag tttatatttt atctgccgta ttttttgtag caaacaaaga atatggaagt
     7921 ctaggtctag tagcaattag tttagttggt actttcattt tgagttttat aaataagcaa
     7981 actgaaagat tattgagtaa agaattatat ataatgcttg ctttatttat aatgttttca
     8041 tcattgttag gtacttgttt taatttttat gatataaatt attatgatga ttttttgcat
     8101 ttatggtcag gtataatatc ttgtattgtg gctttttcta taattaaata cttttataca
     8161 caaagagaga taagtaaaat gagtaaagtg tttttagtta tatttttatt tatgtttagt
     8221 atgggagtag cgagtttatg ggaaataggt gaatttttaa tggatacttt tgttgggact
     8281 actacacagg ccggagggtt agaagatact atgattgata tggtagatgc tttaataggc
     8341 acaattataa ctattccttt tatagtaaaa aaatctaata tatagtttta aattttttat
     8401 attaattata atggatttaa ttaatcatgt tgagtttcaa ggtctatatg attaattaaa
     8461 tccatttttg ttatttataa ttattattga atatattttt acatataaga tataaggtga
     8521 tagatagaaa ttgttggatt atgtaaataa ttaatatata ataccattat gggcaataaa
     8581 tacaaatcaa atgataatat agtctattta tgtaaatgtt atattgtatc ctaagtataa
     8641 tagtaagttt tttgatagtg gtgtagaaag taaaaatcta ctagcctaaa ttgatgtatg
     8701 gattagttta ggaacagtaa ttgcatatct taagtttgat gtcatgttgg gctactagaa
     8761 ataggtcatc tttttcactt gaatatcttt tctttaaaca tggggagtat catatcaaaa
     8821 acatatgaaa attaagtgat ttatgagaac agtaatattt tatatgatag tatagaatag
     8881 taataaagta ttaggaggaa cattgagtgt taaaaaagtg gtttggtatt gtgaaaaaaa
     8941 gacaaaaaag tgagtcagta aaagaagaag gtgaagtaat attaaaaaac gaaaaaatat
     9001 tatctgaaga aaagttgata gatgaagaag gagttgtagt taatattgat aatcaaatat
     9061 taactaaagt agtaaatgat gacaatgaaa tagaagaaaa aataatagaa gaagatttgt
     9121 taattagtga aaataccata aaattagatg ataaagaagc aattattaat gataaaaaca
     9181 tagaattatg taaaaaagaa gttcaagttg aaagtgaaaa aataggttta aataagtttg
     9241 aaggactgga ccaaaatgaa aattataatc tagaaaaaaa tgttattgaa gaaaaaaaag
     9301 taagtgaatg tttgacagaa gaagatttag agtacataaa agaaattaaa ataaaaagag
     9361 gaaaaagtat aaaagctata aacttgtata ctaaagaaga gtgggttttt gacactcata
     9421 tacagtgtag taaaaaactc aaagttccat tagggtacat aagagaaaat ttaaaatatg
     9481 gatatatgga ttactttgga gatgcaataa attatttaag tgaagtatta aatatagatg
     9541 aatactgcaa aagtgaatgg agctatctcg ataatagcaa atctccatct gagatattta
     9601 atattctaaa caataaaata tttagtataa ggctttctaa tgaaaaaaga aatgaaattt
     9661 tgacaaatga taaaattgaa gcattaaaaa tgaattatag atttgaatgt attgatgaag
     9721 aatatgatga atattttaaa aaatataagt ctataatcaa aagaggtgga aagaaaaaag
     9781 ttgaattagt aaataaaaaa ggtgacattt tagaaatatt taagtcttta gaagaatgtg
     9841 ccatttattt gcaaaaggaa aagaatgaag ttatacaaat gttaaaatat ggagatacaa
     9901 aagtaggaag aaactttata aggtatagtt tgagaagtat ttaatttcat tttaaattca
     9961 taagagtttc atttctgatt cacacattta atattatttt aatgtaaaat aataaaggtg
    10021 aagtattgca caattaaaat taaggagggt attaatgtct gaagaaaaaa atccaaaaga
    10081 aaatacatta gatgaatcta aaaattcaga taatgtaaat tctaaaaaag atgatgtgtc
    10141 agatgaaaaa aatactgata acaaagttaa aaaaagggaa ataaagctaa acaacaaaac
    10201 acttaaagct attgtagcaa ttgttgcagt aggagctata tgtttaggtg gaggctatgt
    10261 ttttggcaaa gaagctggaa gaaaattgcc tgctacttca aaacactata atagcagtga
    10321 ggtagtagct actattgggg ataaaaaaat aacaggtgaa atgttaagaa agagaatgga
    10381 gccattattt tatttaaatg caaaaactac tatgactgat gacgaaatac gttcatatga
    10441 atcaaatatg attgaaacta tgattacatc tgatatatta tcctctgaag cagcaaaaga
    10501 gaaaataagt ataactaatg accaactaga tgctcagtat aataatttaa tgagtagtat
    10561 agaatcagca atgggaatga ctaaagaaga gtttttaaaa cagtttgatt tgactgaaaa
    10621 atatataaaa gatgatatga aaaaagagct agtagcatca aaatatttaa atgaaaaatc
    10681 aaaagttact gataaagaag cagagaacta ttacaataaa aacaagagca attatttaca
    10741 agttagagct tcacatattt taataaaaac tgttgatgat aaaggtaagc aagttagtag
    10801 tagcaaaaaa gctgaactta agaaagaagc tgaagaaata cttaaaaaag cccaagctgg
    10861 ggaagatttt gctactctag ctaaaaaata ctctgaagat agtagtgcag aatcaggtgg
    10921 agatttaggt ttctttggta aaggtcagat ggtggaatct tttgaaaaag cagcctttgc
    10981 attgaagaag ggagaggtat ctaacaaact tgtagaaagt gattatggat atcatataat
    11041 taagaaaact gatgaaaaat atcagccatt tgaagaggta aaatcagatt tagtaagcag
    11101 tttaacatca gaaaaacaaa acctacttat acaaaatcta aaagaaaaat ataatgttaa
    11161 aattacagat aaatatcaaa tgaaagctaa agaatagata aagtcaattt attaaaaaaa
    11221 agaaaaatta actgtatgga taaaagctat ttttactctc aatatagaga gtataaaata
    11281 gcttttatta ttttgcaata aaatctattt atatatatga agtgataaag aaataacatg
    11341 caaaaaggta ttaaaaagaa atatttttaa tttgaatttt ttcaataaaa atttacaaaa
    11401 atgaaaaaat aaacaaaaaa gaattgaaga tttaagttgt tatttaaata tatatatatt
    11461 aagattttct gctgaagata ctaaaaaaat aatatttttc ataattttaa aaataataat
    11521 aaaaaaatga aaatacaggc tataatctat tgagtaaaca tagaaaagat gatattataa
    11581 gtatgtgcta aaatggttaa agcaatatta atattaaatt taactataaa acaaaagggg
    11641 gcgttttttt atgtcaagta cagaaagcgt atctaaaaaa agtggtcatc cgaagagttt
    11701 ttggcttatt tgcatgacaa ttttgtggga aagatttagt tattatggtt taacagcttt
    11761 attggtatta ttttttacag caagtgtagc tcaaggtggt ttaggtcttt ctactcaaga
    11821 agcaacatct atgtttggct tatttacagg tcttatatat cttacaccat tagtcggtgg
    11881 ctggttagca gataaagttt taggtcaaca aaaatgtatt tctataggtg cacttttaat
    11941 agcaatagga gatttcgtac tatttgcttc atctacatct attgcaatgg tttgggtagg
    12001 tctatcaata attattttag gaaatggttt ttttaaatca agttgctcaa atatagttgg
    12061 ggatatatat cctaaaactg atgtgtcaag aaaagatgca gcatacagtt tattctatat
    12121 ggcagtaaat gtaggagcat ttatagctcc aataatatgt ggattgcttt ctgataatgt
    12181 atttgcagta agaggagcaa gtggagatat agtatcttat ggatataaga tggcattttt
    12241 aatttgtggt ataggcatga tgttaggtat gattatattt acacttcttg caccaaaatt
    12301 attaaatgaa gtcggaaaat atcctgtgac taaaggtaat aaaggtgaaa aaattgagta
    12361 tggaccactt actaagttgg agaaaaatag gataatagca atggttattt tatttttatt
    12421 tgtggtctta ttctggacag catactatca aacacaaagt tcttttatga tttatgtaag
    12481 ggataatgta aatagaactc tatttggatt tgaaatgcca gtagcatggc ttacatcact
    12541 aaatgctata ttatgtgtgg ctttatgccc agtattagct accctatggg ttaaattatc
    12601 tcataccaaa agaggtgatt tctctattcc agttaaaatg ggattaggaa tgataataat
    12661 gggtgttggg tttatagtta tggtatttgc tacattagct aatggtccag atggaatggt
    12721 aaaggctagt atattgttta tagtattgac ttatatattt acaacagtag gtgaattatt
    12781 cttatctcct ataggacttg caatgttcaa taaattagct ccagctaagt atgctacgtt
    12841 gtctatggga gcctggtatc taacaagttt ttttgcaagt ttattatcag gaaagattgc
    12901 tggttttaca gccacacttg gttttttaca gatatttgga ggaatagcaa cagttttaat
    12961 aatatttggt ttaatattat tgggaataag aaatgtatta acaagattaa tggctatgga
    13021 tttactaaca aaagaaaaat aattcatgac gaattattgt aaataaatga attactttaa
    13081 ataaaggaag ttacatttaa atataaaatt aaatgtaatt tcctctttga ttatgaaaaa
    13141 aagctttaca caaatagata ttataattaa aattataatt gtgataatta gagtgtataa
    13201 gatttatata aattatacaa aaaatattta tgtaataaac tttacatact tatatattta
    13261 gttattcaaa ctattatttc gaggaggtct tgttgtgaat ataaaagata gattaagtgg
    13321 attaagaaaa tttatggaag aaaaaaatat agatgcctat atgattccat cttctgataa
    13381 tcaccaaagt gaatatgtag gagattactt caaatcaaga gaatttatat cagggtttaa
    13441 tggttcagca gggacggtta ttgtgactaa agacgaagct gggttatgga cagatggaag
    13501 atattttata caggcagaat ctcaattaga aggaagtaca ataaaattat tcaaaatggg
    13561 acaagaaggt tgcccaacta cagatgaata tttatataaa aatattcctg aaggaggaac
    13621 tttaggattt gatggtagag ttatatctgc tagagaagga gctacattag ctgaaaaatt
    13681 atctaaaaaa ggtataaaga tagaatatca gtatgattta atagatagta tatggccaga
    13741 tagacctgct ttatcagata gtaaagcatt tttattagat gttaaatatt gtggagaaag
    13801 ttttagctca aaattagcta gattaagaga aaaaatgagt gaaaaaggta cttctactca
    13861 tgtaataact actcttgatg atatagcatg gttattcaat ataagaggtg gagatgttaa
    13921 atataatcca gttgtacttt cttatgcagt aataacttta aaagaagttt atctatttgt
    13981 tgatgaaagt aagttaaatg aagaaatact aaatgaacta gctaaggaaa atgttcaaat
    14041 aaagccatat aatgatgtgt atgaatttgt aaaaaatata gacaaaactg aaaaagtttt
    14101 attagatgga actaagctta gttatacaat ttacaataat ataccttgtg aagttgagaa
    14161 agttgatgag ttcaatccag taatgttttt taaagctcaa aaaaatgagg tagaattaga
    14221 aaatataaga aattcacatg taaaagatgg tgtagctttc actaaattta tgtactggtt
    14281 aaagaaaaat gtaggtaaga tggaaatcac tgaaataagt gctactcaaa aattagagga
    14341 cttaagaaga gagcaagaag gtttctttga gccaagtttc aatacaattg ctgcatataa
    14401 agagcatgct gcaatgatgc actatagtgc aactccagag tcaaattata aattagaggc
    14461 agaaggatta tttttagtag attcaggtgg acaatattat gatggaacta ctgatataac
    14521 taggactaca gttttaggac ctataagtga tgaattaaaa ctacacttta cttctgtagc
    14581 aagaggaatg ataaatcttt ctaaggctaa gttcttgcat ggttgtagag gatataactt
    14641 agacatacta tctagaagtt gcatgtggaa tatgggaatt gattatcaat gtggaactgg
    14701 acatggtata ggatttgtgc taaatgttca cgaagctcca aatggattta gatggagagt
    14761 agtacctgaa agatttgata gtgcagttct tgaagaagga atggtaacta ctaatgaacc
    14821 aggtatatat atagaaggtt ctcatggtat aagaacagag aatgaaattg tagttagaaa
    14881 agctgaaaag aatttctatg gtcaatttat ggaatttgaa gttgttacgt tagctcctat
    14941 tgacttagat ggtatagcac cagaattaat gaataaggat gaaaaagatt atttaaattg
    15001 gtatcataag ttagtttatg ataaaatatc acctttctta actgatgaag aaagagaatg
    15061 gttaaaagtt tacactagag ctatatagtt tgtactagat acaataaaaa tcgaggtatt
    15121 ataatgcctc gattttttat tgtatatttt attaacttta aaaaattttt aatttgataa
    15181 ttctactaat tttttattca ttataagtag tataattcca aatatcaata aaaatgatgg
    15241 tatcactatg aagacttgta acataccaag ttggtctata atagcttgcg tatagccaga
    15301 taatttattt gctgcaaatt tgcctaaaaa ccaaacaccc attaggaatg aaatatactt
    15361 ttcaggcgaa agtttattta ccataccata cccgactgga gaaaagcaaa tttctcctat
    15421 actttggaat gttaaaaatc caactaccca aaatacactt gctttgtgaa ttgaattagg
    15481 gtctgttcct gtttgtataa ctgctactat cataaacaaa aatgctatgg ctaatagtat
    15541 aaaacctaag ctcatttttt ttggcacact taaatcgcct tttttagttt gagataattt
    15601 tacccatact aaagcagaga taggtccaag aataacacaa aggagaccat tgtatgaatc
    15661 tatccatgaa gtaggaattt gaaaattgcc aacagtaaga tttatgtgtt cctttgtata
    15721 gagtgcaata gacatagagg cttggttata tgctatccaa aaaaatactg tgaaaaaagc
    15781 taacaccaat attactatta ttcttttttt ctcaatcaaa gttaaaggtc ttgaagcgag
    15841 cattttctta tattctagtg attttcttat agactctctc ccgttaatag ggtcattatt
    15901 caaaggttta taaaactttc tgtttccaaa tataaagaag aataaaccta ttagaaccgt
    15961 aattgatgaa aataaaaata catatctata tccatacata cttatagata atccgctttt
    16021 catagcaaac cattcattag caactaaacc gcatattaaa gggccaaata aagtacctag
    16081 attagtagcc atatacgtaa tagagaaagc tccatctttt cttgggtcat cattggtata
    16141 taagtttcca accatagtat gtaagtttcc tttataaaaa cctattccta atccagataa
    16201 aaatatcatg atgtttatat ccatcttact atttgctatg tatcctagaa aataaccaat
    16261 tgaaaccatt agcattccaa gtataataca gtttttggga cctaaaaatc tatcactgac
    16321 ccatccacca ataactggag agaaataagt ccatgctaaa aaagatgcaa gcattgatgc
    16381 ggattctgct ggtgttaacc ctaaaccacc tttggagact tgttcagcta aaaacaataa
    16441 taataccaat ttcataccat agtatgcaag tcctacaaag gtaaatattg aacagcagaa
    16501 atataaatct ctaggttgtt taagtttttc ttgcgttttt aaaactgaca tatgtcgaga
    16561 acctccagat aaatagttta acaaaatttt accatactat atgagtatat gtttttttca
    16621 tatagacgtc aacttgtaag ataatttaat taagtcgaaa tatatgtaaa aatagtatca
    16681 tttttgtgta ttcagtaata tttttttatg ataatatatg ttattattat attaaaatac
    16741 aaacatattt atggaaagga tatgccaatt tagtagttag attgattgtt tggcaggtgg
    16801 gatgagctat gataagaaag aaatctgaat tacaagtaga agtaaaagaa aatttaaatg
    16861 gtggtatagg aaaggttaag cttgaaaaca taatacaaaa tgaagagctt aaaggaaaag
    16921 gaagactatt taaaagagta gctttaccag taggaagttc aattggtgtg catgaccata
    16981 caacagattt tgaagtatat tatatactaa agggaaaagg taaagtattt gataatgggg
    17041 aatttgtaga agtaaatgag ggtgatgtag tgtatacagc tgatggcgaa aaacattcta
    17101 tagaaaatat aggagaagaa gatcttgagt ttgtagcact agttttatat atgtaaaata
    17161 tggaggagga gtcatgacag gcaagataga agaaattaag gacaagataa atagattttg
    17221 caactccaat ttaaatcaag aatacaaaga tataagcttt aaaattttgc aaaacctttt
    17281 agataataat aaagagataa ttcattcttc tagagctgat atatggtctt cagctatact
    17341 aaatatagtt ttagaacaaa atcttttata caataagaag catccactac atataacaaa
    17401 aaaagagttt tcaaaacaaa taggtgtttc tttaaatact ataaataata aatcgtcttt
    17461 aataaaagat gtttgtagta ttgacttttt aaatcaagat actatagcta tttctaatgt
    17521 agaattttgg gtaaaagaga ctaattccaa atcaaagtct gtagatttag atttagaaaa
    17581 gaagaaaata ctatataaaa gatatattaa gctatcacaa gatgcgaata gtgatattga
    17641 atcaattaga tatatggagg aagctgtaaa tataggtaaa agtatgatta ctaaagaaga
    17701 taaagaactt ggattttgga aaggtttatc taccagagcc tatatgatag cacttgaatc
    17761 ccttgctaga aagttagaaa gtattaataa tctaaaggag tccaaaaaag tattagaata
    17821 tttaatagaa attaatccta gtgatgagca gggaataaga tacaaattat taaatgtatt
    17881 aattagatta aatgatagaa cttcaataaa taaattattt gaactttata aagaagaaaa
    17941 atctgctaca tggatgtaca gtaaagctct ttattacttt aaaaataaaa gtatgttttt
    18001 ggcaaatgat tctattaagg ttgctagaag taaaaataag tacgttggat tatatttgtt
    18061 agattggcga aatgcttttg gtagagaatt tgttacagag gaagaaaagg cagaagctgt
    18121 ctattactat gatgaaaaca tagtgatttg gaatgaagtt aaaggttcta tggattggct
    18181 attaaaaaaa atgttggaat tttcataaaa aaaactactc cttactttta tataagtcaa
    18241 aggagtagtt tttaatttat acatctagtt ttaaatcttc aggttttatt agaccttttt
    18301 ttctcataaa ttctgaaatc aacaaagtta caatagcagg aagtataaaa ttaagtaaaa
    18361 gtataccaac ccacatagat atacctcctt ttccagcact ttgcatagca gttactgttc
    18421 caaattgtcc aaccaatcca caagtaccca taccagaacc aataggaata ttctccattt
    18481 tgaatattgt tgtagataat ggtccaagta atattgaagc tacagtaggt ggtatccaaa
    18541 ttttccaatt ttttattata ttaggtactt gcaacataga tgtcccaaga ccttgtgcca
    18601 caaggccacc aactccattt tctctaaagc tcataacagc aaagcctacc atttggcaac
    18661 aacaacctac agttgctgca ccaccagcaa gaccacctaa acttaacatc atacaaatag
    18721 ctgcactact tataggtaat gtaagagcta ttccaacaag tgcagagact aaagctccca
    18781 ttattatagg ttgcatagtt gtagcttgca ttattatatt tccaaatgaa gtcataaaag
    18841 cagaaacagc tggaccaata agttcagcta caagtactcc aattagtatt gttacagcag
    18901 gtgtaaccaa tatatctatt tttgtttctt ttgaaactaa ttttccaacc tctacagcaa
    18961 aaagtgtagc gacaaatgct ccaacaggtc ctcctaattc attaccacaa gctccaacaa
    19021 gtgcacaaga aaatagtaca agtggaggtg cttgaagtcc atacgcaata gccactgcta
    19081 tagctggacc agtaacctgt ttagcaagag ggcttataac ttcagtaaaa agagaaatat
    19141 tgaaattatc tcctaatgta ttgaaaattg ttccaattaa aagggacgaa aaaagaccga
    19201 gtgccatata acttagtgcg tcaataagat acctttgaac agaaatttca atattttttc
    19261 ttttagcaaa tgatttgaaa tttctttttt tataattgat ttccaattta aaatcctcct
    19321 catataatat aagtataatt tctttttaat taattatata acttttcagc tgacaagaca
    19381 aatgttattt cactaaaatt agactattgt aatagtaaga aattaacatt aattttagtt
    19441 ttaaatttta aatttatggt ttataattag tatagggata ttctgaaata taataatttt
    19501 ccagtttatc ccaatattta acttaggtag attgcttaca aaacaaatga ttatatatta
    19561 ggctaaaagg aagtggatag atgatatttg taaaaaataa gaaatacaga aaaattgcca
    19621 taattatatc tattcctata ttgcttgttg cgcagtacta cctttttaaa tttggaatac
    19681 ttagaccaat ggtaaaggga gtagaaattg aaattgtaga tggggaatat gttaaagata
    19741 tagataaata tgtagtaaaa ttaaatgaaa cagttacttt gtctacaggt gaatatatta
    19801 aggttccttc gtatgctaaa aatccaaata tttggtttaa tgtattggat aactcaggga
    19861 ctttaaaaat agatggaaat aaaatgacag cattaaaggt tgggtattct tctgtgggta
    19921 tcatgaaaaa ttcaagagta ttaaaaaagg ctactataaa agtagtagac ccagaagtag
    19981 aatcactaga tatggagatt aatggggatt taaaacatgt tggtgatagt gctactatag
    20041 aaagtacaat agaagttgat tatgataagt ttaaaaaatc atataagcca gtttataaga
    20101 gtagcaatga aaatatttta aaaatcaaag gaaataaagt aagtgcagtt ggagttggta
    20161 aagctacaat atatgctaag tgtggaaata aagaaattga gagaacattt aggattcaag
    20221 ctagagttgc tgaaataaat atggatgata taaaaataga agttgaccag aatgaaaaat
    20281 tgaaaccaga tataataact tctccgcctg gtttagaacc accaaaaata tattatgagt
    20341 tagtagattc aaaattacct gtagaaagag caataagttt aagtagtaat ggaaatgttg
    20401 tagggcttag agaaggtaaa gatagggtga gaataacttg tggagataaa tctaaagtaa
    20461 taacagtaac agtagtcaaa gaaactataa cgaatgacta tatacaaaac ttggttggtt
    20521 cacataaaat agtaaacaat aaagttatta ttaaacttga atgggattat atgaaagatg
    20581 tctttgatta tgatatatat cttaaagata agttgtcagg agaatcattt aagaaaatta
    20641 aatctataac tataaatgaa agtgatttag gtaagtctaa caaggtatat gcaactatag
    20701 aagtagatat gaagggtaat caaaatatag attttgatat ttatgtcata gggaaaaagg
    20761 attcagatac aactaagcca agtaatgtaa taaatattac caatggaaat ggaaattcaa
    20821 ataatgataa taatattaaa gatatgaggg tagaaaatat atcagctaat gttgatagag
    20881 ataataatgt cattagagtt aactggagta gtatatctca taaggactgt acttacagta
    20941 tatatgtaaa aaataatact aatggagatg gagaatttac tctatatcaa agtgatatac
    21001 agggaaatga gcatactata agtattcctg aaggagattt aaatctagat atttatgttg
    21061 ttgcaaatta taatggaaat agctctcaaa atagtgatgt tataaatgta agatagattt
    21121 gtatacaaat tgaaagtgtt gagtttattt aaatattaaa ttaagaaaaa ggatagtata
    21181 gggtaattat actatccttt tttgttacaa tatataggtt taaaatctct agtaatataa
    21241 attataaaaa gtcttattta cacaaatcta tagatatttt ttttattaat caatattgtc
    21301 ttaaattgaa cttagtgcgg cttctattgc atgtattact gtcgtatcaa atagtggaat
    21361 agaagtgtca ttttgtttaa ttaaaagacc tatttctgta catcctaata taacagcctc
    21421 tgcaccttgt ttgccaagct tatctataat agataagaaa gctttttttg aagattcaga
    21481 tataattcct aagcaaagtt cattaaatat tgtgtcattt actattatac ggtcttcttc
    21541 atttggaatt aagacttcaa ttccattatt aataattaca tttttataaa aatcttgttc
    21601 cattgtatat ttagtaccta ataaggccac tttttttatt tctttttccc ttaataccgt
    21661 agcagttaca tctgctatgt gaagtaatgg aatatgtata ctttcttgta ttttatctga
    21721 taccttatgc atagtattag tacagataac aataaaatct gcccctgcct cttgtaattt
    21781 aattgcagca tcagctaaaa ttttggcgct tttttcccaa tttccactag attggcattc
    21841 ttcaatttct tgaaaatcta cactgtaaag tatacattta gaagagtgta gtccacctaa
    21901 tctttctttt attactgtat taataacttg ataatatgta atggtacttt cccaactcat
    21961 accaccaatt aagcctatag tcttcatatt atacctctct tatttattta ttttgaaata
    22021 gctatttaca aatacaagta tagcatctaa aatagaagca tacaatgata aaattgtatg
    22081 gagtacaatt ataattaaga ggagtaatta tatgccaata aattcttttg aaaactatcc
    22141 tatgacatgg aaaccaaaat tagataaccg aaaaccacca atatataaaa ctttagcaat
    22201 gttattagaa gaagatatta aaagaggaaa tttaaatcct ggagataaac tgcctccaca
    22261 acgggaatta gcagattttc tagatttaaa cttaagtaca ataacacgag catttaaact
    22321 ttgcgaagaa aagggactaa tatgtgcaaa agtgggaaag ggaacattca tttcttctga
    22381 tgtaaatgta agtaatacat tattatatca aacggaatca aaagacataa ttgaattagg
    22441 gacagttcat cctccgtatg agcaaaatac atatattatt gactttatta aaaatgtact
    22501 aaaacaacca gaaatggata ggtttttaca atacatgtct ccatctggca catatatgca
    22561 aaaaaagtca attgcaaaat ggatagaaag aaataatgta tacaccagtg aagaaaatat
    22621 attattatct acaggagggc aaaatgctat atgtgcaact ttactaggat tatttaaagc
    22681 tggtgataga attgctacag attctttaag tttttctgga attaagtcaa tagctaaaat
    22741 gataggtatt caattagtcc caattcctca agaaaataat gaaatatcga ttgaatatct
    22801 tgaaagttat tgtaaaaatg aaaatataaa aggcatatat cttataccag attatcataa
    22861 tccaacgaca catactatgt ctgatttatc tcgtaaaaag atagccaaga tagcaaagca
    22921 atataatata ataattattg aagatgctat aaatagcatt tttagagatg gtatacaaac
    22981 tcccatattt tctttagcta gtgataatac tatatatatt tttagtacat ctaaattttt
    23041 atgtgctggt cttagagtag catttgtagt tgctccaaaa cgatatatag aaaatttaga
    23101 aaatgcacta tataatatga atctaatggt ttctccattt acagcagaaa tagtacaccg
    23161 tttattatat tctcctatta tagataaaat tatagaagag aaaagagatg caattataga
    23221 aagaaatgaa gttgctgata aagttttgtc agattataat ttgattggtg ataagaattg
    23281 cagttttcgt tggttacttc ttcctgatga attggatgga aaatcatttg aaatttctgc
    23341 aaaaaatcta ggagttcaag tatattgtgc tgaacgattt tcagtaggaa actctacagt
    23401 tccaaaagca gttcgtattt gtgtaacagc tccaaaagat gtagaagaat tagaaaaagg
    23461 gcttaatata ataaagtcac ttcttaagag ttaaatttat tttactatta tttaattaaa
    23521 taaataaaaa ggcggagaaa tttattataa taaacctccg cttttattat ttaatgaaat
    23581 actttacttt ttattaaaaa atttaatttt aataatcttg aaaatataat cgttttatgg
    23641 taaaataatt cttaaaaata gaaaaaatta atcatttgtt aaaaaaaatg cataatttat
    23701 tgtaatttat tgacgaaggt tgaaaaatat tcataaatgt gcgaaataac agaaaaaatg
    23761 caaaaattaa ttcaatttca tttgttataa aatctatatg gcaaaattaa atctgcctat
    23821 cagaatgata taaacacacc agaaaggagg acgctatcaa ataatacaag tagttaactt
    23881 tgatagcaaa aaattatgaa tgaacaaact agaatatcct tagagagagc tgctgaatta
    23941 aaatcaaaaa ttgatgatta tattcaggct agaaaaacga ttaacagagg tcttgaaaaa
    24001 gaagaagaga taaataaacg aaaacagaaa atattaagta tcttaaatgg aactgaagag
    24061 gattggaata actacaaatg gcaattatca aatagaataa cagatgtaga tactttatca
    24121 aaaattataa ctctaactaa aaaagaaaaa gaatatataa aagaggttgg tactcaattt
    24181 agatgggcaa tatctccata ttatttgagt cttatagacc cagaagatac atgtgaccca
    24241 ataaaattac tgtctatacc aacacatata gagttggaag atgaacaaga agatttggac
    24301 ccaatgggag aagagtatac aaacccagca ggatgtataa ctagaagata cccggataga
    24361 ttaataataa atgtaacaaa tgagtgtgct atgtattgta gacactgtca gagaagaaga
    24421 aatattggac aacaagattc tcataagtca aaagctatta tccaagaatc tatagactat
    24481 atcagagaaa atgaagaaat aagagatgta ctagtaactg gtggagatgc tcttacatta
    24541 aaagatgatt atttagagtg gattcttagc caacttaaag agataccaca tgttgattat
    24601 gttagattag gtactaggac tcttgttaca atgccacaaa gaatcacaga tgaattttgc
    24661 aatatgctaa aaaaatatca ccctgtatat ataaatactc attttaatca tccaatggaa
    24721 ataactaagg aatctaaaga agcttgtgaa aagttagcaa atgcaggagt tccattagga
    24781 aatcaggcag tattattaaa tggaataaat aatgataaat ttgtaatgag atgtttaaat
    24841 caagaattac tgaaaataag agtaaaacct tattatatat tccaaagtaa acatgtaaag
    24901 ggaacaaaac atttcaatac atcagtagat gatggtcttg aaatcatgga gtatttaaga
    24961 ggatatacat caggaatggc tataccaaca tatatagtaa atgctccaaa aggaggcgga
    25021 aagactcctt tgcttcctca ataccttgta tcaaaaggaa cagattacgt tatgcttaga
    25081 acatgggaag gaaaagttat aaaaatggaa gatgaacctg ctgtagatat aaagaaactt
    25141 ataaaagaac aagcgcagga ttaaaaaagt atttaaagtt tataaaaaga aacaacttta
    25201 aaataaaaag aaaatggatg tcaaatttat atcaatttgc atccattttt tattagttat
    25261 ttattatttt gttgttgatt aactaattca agatatccac ttgggtggaa gtaatatatc
    25321 tcagaagtat tttcatttac aaagtaacca atatcaccat ctgcatctgg taaaaataca
    25381 taaccattaa gatttttagc ttttaatgct tcaaaatcac tagcatttcc ttgatatatg
    25441 taatcaactt cattgttgta ttttactaaa agttctttag cttgttcttg agtcaattta
    25501 gtattttggc ttacaggaac attcttttga gaatctgcat aagaagattc atttactggt
    25561 gcagttaata acaaacaaga taaaactaat aaagatataa gtgctttttt catactaaaa
    25621 ccctctttca tgtttaatta tataagattc attacttaat aatattgtat gtttcctata
    25681 attaaatatt accattaaag agaataatat tacagttaca cagctgtaac ttttgattac
    25741 aaaatcgtaa ttaaattgta tttttattcc atttgtattt tatcatgtct acgatttaca
    25801 aactcacaga agtatatctt tataacgccc atcacaggag cagatagaat cataccaggt
    25861 ataccaaaga atcctccacc aacagtaact gctaggattg taaagaatgg gctaagacct
    25921 agttgtccac ctacgatttt tggctctata aatgtaactt caacttgttg aattattatt
    25981 aagtaaatta aagaagttaa agcaattgtt ggattggaga ataaattaat tattacaact
    26041 ggtgccatac ctattactgg accaaaatat ggaaccatat tcatgacacc cataagtgta
    26101 ccaaataata gtgcatattc agattttaaa aagtaaagac caattccaga aagtactcca
    26161 actataaaag aatctaaaat cttacctgta aagtatttac caatatttaa atttaagctt
    26221 tgacatagtt ctattataaa gtctccatat tttttaccta aggaaatata aactactttt
    26281 tttgtaaaca gtaagaattt ttctttttct aataatatat agaaacaaat tataaaacct
    26341 aaaagaaatt gtactataaa ttttccaact gaaaatgtag tgctaaatat cccaccaagt
    26401 gaaccaataa gcaagttact aaatttaggc attacactca taattttgtc accatactct
    26461 tttaatgtat tagggtcaac tgattgtaaa ctcttgccta agtcgaataa aaactgctct
    26521 gttttatcta tgtatgtagg aatttgagca actaaatcag ctaaactatt tactatggat
    26581 ggaactgtaa ataatataaa cgaaattagt ataactatga gtacaccata agtaagaagt
    26641 aacgaatata ttctcttaaa atttaattta ctttctaaga actttactag tgggttgaat
    26701 atatatgcta atacaaatgc tattataaat ggtgttagta gtgacattaa taaacttatc
    26761 actccaaaaa aatatttgta attatcaata actttaatca agatatatga aacaactaca
    26821 gtgagtacaa tatttagata aattttttta tcttgcaaaa taacacaacc tttcttatat
    26881 ttattaaatt tagatatact taaatctaag ttaaagttaa tatttatagc ttattataca
    26941 ataaaaagca gtattaatat agtatgatta atgtgaaatt ataaatacgt taaaacttta
    27001 tgtttatagc taaaataata aaaattattt atattgtgtt tatatataat ctaaaatgaa
    27061 aaaaagtggt gcctttgatt aaactaagtt tttataa
//
LOCUS       DAYAOY010000051        26061 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYAOY010000051 DAYAOY010000000
VERSION     DAYAOY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.051"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            575..1129
                     /gene="tcdR"
                     /locus_tag="ACGI6B_002256"
     CDS             575..1129
                     /gene="tcdR"
                     /locus_tag="ACGI6B_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HFG9751698.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1447..8547
                     /gene="tcdB"
                     /locus_tag="ACGI6B_002257"
                     /gene_synonym="toxB"
     CDS             1447..8547
                     /gene="tcdB"
                     /locus_tag="ACGI6B_002257"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HFG9751699.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8669..9169
                     /gene="tcdE"
                     /locus_tag="ACGI6B_002258"
     CDS             8669..9169
                     /gene="tcdE"
                     /locus_tag="ACGI6B_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HFG9751700.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            9248..9409
                     /locus_tag="ACGI6B_002259"
     CDS             9248..9409
                     /locus_tag="ACGI6B_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751701.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9474..9680
                     /locus_tag="ACGI6B_002260"
     CDS             9474..9680
                     /locus_tag="ACGI6B_002260"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="HFG9751702.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9897..18029
                     /gene="tcdA"
                     /locus_tag="ACGI6B_002261"
                     /gene_synonym="toxA"
     CDS             9897..18029
                     /gene="tcdA"
                     /locus_tag="ACGI6B_002261"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HFG9751703.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(18364..19062)
                     /gene="tcdC"
                     /locus_tag="ACGI6B_002262"
     CDS             complement(18364..19062)
                     /gene="tcdC"
                     /locus_tag="ACGI6B_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HFG9751704.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            19488..19733
                     /gene="cdd1"
                     /locus_tag="ACGI6B_002263"
     CDS             19488..19733
                     /gene="cdd1"
                     /locus_tag="ACGI6B_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HFG9751705.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19951..20220)
                     /locus_tag="ACGI6B_002264"
     CDS             complement(19951..20220)
                     /locus_tag="ACGI6B_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9751706.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(21098..21835)
                     /locus_tag="ACGI6B_002265"
     CDS             complement(21098..21835)
                     /locus_tag="ACGI6B_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751707.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            complement(21847..22608)
                     /locus_tag="ACGI6B_002266"
     CDS             complement(21847..22608)
                     /locus_tag="ACGI6B_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751708.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            complement(22608..23531)
                     /locus_tag="ACGI6B_002267"
     CDS             complement(22608..23531)
                     /locus_tag="ACGI6B_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751709.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            23693..24400
                     /locus_tag="ACGI6B_002268"
     CDS             23693..24400
                     /locus_tag="ACGI6B_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9751710.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            24445..25851
                     /locus_tag="ACGI6B_002269"
     CDS             24445..25851
                     /locus_tag="ACGI6B_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9751711.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
BASE COUNT        10221 a         2999 c         3930 g         8911 t
ORIGIN      
        1 attaataaac aaaaaaaaaa ttatcttaag agaggagaat gttctaaaat ataaaaaggt
       61 ttctagattt cataaaagat actattttag tcttgaaaat atttagtttg aaaagatttt
      121 aatttaatga ttgattaagt taaaaatgtg tatgtaataa attttatttt atttattgac
      181 taaattataa agtttacata attatttaat aattatgtaa ttgttacttg aaaattgatc
      241 tattttaaaa tctagttata acttcaaaaa agactgaaaa ttaagaaaaa agaaatataa
      301 atataaaaat atgtcatata gatttttttt attttacttt aataaaatga tttgttttta
      361 caatacttta ttaatataaa gtttattgct aaaatacttt atttattaga aaaagattac
      421 taattaatta ttaaaattaa tgtattcata atgtatattt tcatataaaa tttaatttat
      481 ttgccgatta tataattata atgactgatt taattccaat gttgtcaaaa ttttcaaata
      541 aatcatcatt ataaatataa gagaggatga ttttatgcaa aagtcttttt atgaattaat
      601 tgttttagca agaaataact cagtagatga tttgcaagaa attttattta tgtttaagcc
      661 attagtaaaa aaacttagta gagttttaca ttatgaagag ggagaaacag atttaataat
      721 attttttatt gaattaataa aaaatattaa attaagtagc ttttcagaaa aaagcgatgc
      781 tattatagtc aaatatattc ataaatcatt actgaataag acttttgagt tgtctagaag
      841 atattctaaa atgaagttta attttgtaga atttgatgaa aatatcttaa atatgaaaaa
      901 taattatcaa agtaagtcta tttttgagga agatatttgt tttttcggat atattttgaa
      961 agaattatct ggtattcaaa gaaaagttat tttttataaa tatttaaaag gatattctga
     1021 tagagaaata tcagtgaaat taaaaatatc tagacaagct gttaataagg ctaaaaatag
     1081 agcatttaaa aaaataaaaa aagactatga aaattatttt aacttgtaat taatgagttt
     1141 aaagaaatat ttacaataga aatcaaattt tataattaac tttattgtaa aattaataac
     1201 ttaatctaag aatatcttaa tttttatatt ttatatagaa caatgtttac atatttattt
     1261 cagacaacgt ctttattcaa tcgaagagca aattaatcaa ctgagtgtct taaatttaaa
     1321 atgttaggaa gtgaatgtat atgaaaacct aagtagatat tagtatattt tataaataga
     1381 aaggaggata tataaaagag ttttagcatt tagatgtaaa aatattatag taaaggagaa
     1441 aattttatga gtttagttaa tagaaaacag ttagaaaaaa tggcaaatgt aagatttcgt
     1501 actcaagaag atgaatatgt tgcaatattg gatgctttag aagaatatca taatatgtca
     1561 gagaatactg tagtcgaaaa atatttaaaa ttaaaagata taaatagttt aacagatatt
     1621 tatatagata catataaaaa atctggtaga aataaagcct taaaaaaatt taaggaatat
     1681 ctagttacag aagtattaga gctaaagaat aataatttaa ctccagttga gaaaaattta
     1741 cattttgttt ggattggagg tcaaataaat gacactgcta ttaattatat aaatcaatgg
     1801 aaagatgtaa atagtgatta taatgttaat gttttttatg atagtaatgc atttttgata
     1861 aacacattga aaaaaactgt agtagaatca gcaataaatg atacacttga atcatttaga
     1921 gaaaacttaa atgaccctag atttgactat aataaattct tcagaaaacg tatggaaata
     1981 atttatgata aacagaaaaa tttcataaac tactataaag ctcaaagaga agaaaatcct
     2041 gaacttataa ttgatgatat tgtaaagaca tatctttcaa atgagtattc aaaggagata
     2101 gatgaactta atacctatat tgaagaatcc ttaaataaaa ttacacagaa tagtggaaat
     2161 gatgttagaa actttgaaga atttaaaaat ggagagtcat tcaacttata tgaacaagag
     2221 ttggtagaaa ggtggaattt agctgctgct tctgacatat taagaatatc tgcattaaaa
     2281 gaaattggtg gtatgtattt agatgttgat atgttaccag gaatacaacc agacttattt
     2341 gagtctatag agaaacctag ttcagtaaca gtggattttt gggaaatgac aaagttagaa
     2401 gctataatga aatacaaaga atatatacca gaatatacct cagaacattt tgacatgtta
     2461 gacgaagaag ttcaaagtag ttttgaatct gttctagctt ctaagtcaga taaatcagaa
     2521 atattctcat cacttggtga tatggaggca tcaccactag aagttaaaat tgcatttaat
     2581 agtaagggta ttataaatca agggctaatt tctgtgaaag actcatattg tagcaattta
     2641 atagtaaaac aaatcgagaa tagatataaa atattgaata atagtttaaa tccagctatt
     2701 agcgaggata atgattttaa tactacaacg aataccttta ttgatagtat aatggctgaa
     2761 gctaatgcag ataatggtag atttatgatg gaactaggaa agtatttaag agttggtttc
     2821 ttcccagatg ttaaaactac tattaactta agtggccctg aagcatatgc ggcagcttat
     2881 caagatttat taatgtttaa agaaggcagt atgaatatcc atttgataga agctgattta
     2941 agaaactttg aaatctctaa aactaatatt tctcaatcaa ctgaacaaga aatggctagc
     3001 ttatggtcat ttgacgatgc aagagctaaa gctcaatttg aagaatataa aaggaattat
     3061 tttgaaggtt ctcttggtga agatgataat cttgattttt ctcaaaatat agtagttgac
     3121 aaggagtatc ttttagaaaa aatatcttca ttagcaagaa gttcagagag aggatatata
     3181 cactatattg ttcagttaca aggagataaa attagttatg aagcagcatg taacttattt
     3241 gcaaagactc cttatgatag tgtactattt cagaaaaata tagaagattc agaaattgca
     3301 tattattata atcctggaga tggtgaaata caagaaatag acaagtataa aattccaagt
     3361 ataatttctg atagacctaa gattaaatta acatttattg gtcatggtaa agatgaattt
     3421 aatactgata tatttgcagg ttttgatgta gattcattat ccacagaaat agaagcagca
     3481 atagatttag ctaaagagga tatttctcct aagtcaatag aaataaattt attaggatgt
     3541 aatatgttta gctactctat caacgtagag gagacttatc ctggaaaatt attacttaaa
     3601 gttaaagata aaatatcaga attaatgcca tctataagtc aagactctat tatagtaagt
     3661 gcaaatcaat atgaagttag aataaatagt gaaggaagaa gagaattatt ggatcattct
     3721 ggtgaatgga taaataaaga agaaagtatt ataaaggata tttcatcaaa agaatatata
     3781 tcatttaatc ctaaagaaaa taaaattaca gtaaaatcta aaaatttacc tgagctatct
     3841 acattattac aagaaattag aaataattct aattcaagtg atattgaact agaagaaaaa
     3901 gtaatgttaa cagaatgtga gataaatgtt atttcaaata tagatacgca aattgttgag
     3961 gaaaggattg aagaagctaa gaatttaact tctgactcta ttaattatat aaaagatgaa
     4021 tttaaactaa tagaatctat ttctgatgca ctatgtgact taaaacaaca gaatgaatta
     4081 gaagattctc attttatatc ttttgaggac atatcagaga ctgatgaggg atttagtata
     4141 agatttatta ataaagaaac tggagaatct atatttgtag aaactgaaaa aacaatattc
     4201 tctgaatatg ctaatcatat aactgaagag atttctaaga taaaaggtac tatatttgat
     4261 actgtaaatg gtaagttagt aaaaaaagta aatttagata ctacacacga agtaaatact
     4321 ttaaatgctg cattttttat acaatcatta atagaatata atagttctaa agaatctctt
     4381 agtaatttaa gtgtagcaat gaaagtccaa gtttacgctc aattatttag tactggttta
     4441 aatactatta cagatgcagc caaagttgtt gaattagtat caactgcatt agatgaaact
     4501 atagacttac ttcctacatt atctgaagga ttacctataa ttgcaactat tatagatggt
     4561 gtaagtttag gtgcagcaat caaagagcta agtgaaacga gtgacccatt attaagacaa
     4621 gaaatagaag ctaagatagg tataatggca gtaaatttaa caacagctac aactgcaatc
     4681 attacttcat ctttgggggt agctagtgga tttagtatac ttttagttcc tttagcagga
     4741 atttcagcag gtataccaag cttagtaaac aatgaacttg tacttcgaga taaggcaaca
     4801 aaggttgtag attattttaa acatgtttca ttagttgaaa ctgaaggagt atttacttta
     4861 ttagatgata aaataatgat gccacaagat gatttagtga tatcagaaat agattttaat
     4921 aataattcaa tagttttagg taaatgtgaa atctggagaa tggaaggtgg ttcaggtcat
     4981 actgtaactg atgatataga tcacttcttt tcagcaccat caataacata tagagagcca
     5041 cacttatcta tatatgacgt attggaagta caaaaagaag aacttgattt gtcaaaagat
     5101 ttaatggtat tacctaatgc tccaaataga gtatttgctt gggaaacagg atggacacca
     5161 ggtttaagaa gcttagaaaa tgatggcaca aaactgttag accgtataag agataactat
     5221 gaaggtgagt tttattggag atattttgct tttatagctg atgctttaat aacaacatta
     5281 aaaccaagat atgaagatac taatataaga ataaatttag atagtaatac tagaagtttt
     5341 atagttccaa taataactac agaatatata agagaaaaat tatcatattc tttctatggt
     5401 tcaggaggaa cttatgcatt gtctctttct caatataata tgggtataaa tatagaatta
     5461 agtgaaagtg atgtttggat tatagatgtt gataatgttg tgagagatgt aactatagaa
     5521 tctgataaaa ttaaaaaagg tgatttaata gaaggtattt tatctacact aagtattgaa
     5581 gagaataaaa ttatcttaaa tagccatgag attaattttt ctggtgaggt aaatggaagt
     5641 aatggatttg tttctttaac attttcaatt ttagaaggaa taaatgcaat tatagaagtt
     5701 gatttattat ctaaatcata taaattactt atttctggcg aattaaaaat attgatgtta
     5761 aattcaaatc atattcaaca gaaaatagat tatataggat tcaatagcga attacagaaa
     5821 aatataccat atagctttgt agatagtgaa ggaaaagaga atggttttat taatggttca
     5881 acaaaagaag gtttatttgt atctgaatta cctgatgtag ttcttataag taaggtttat
     5941 atggatgata gtaagccttc atttggatat tatagtaata atttgaaaga tgtcaaagtt
     6001 ataactaaag ataatgttaa tatattaaca ggttattatc ttaaggatga tataaaaatc
     6061 tctctttctt tgactctaca agatgaaaaa actataaagt taaatagtgt gcatttagat
     6121 gaaagtggag tagctgagat tttgaagttc atgaatagaa aaggtaatac aaatacttca
     6181 gattctttaa tgagcttttt agaaagtatg aatataaaaa gtattttcgt taatttctta
     6241 caatctaata ttaagtttat attagatgct aattttataa taagtggtac tacttctatt
     6301 ggccaatttg agtttatttg tgatgaaaat gataatatac aaccatattt cattaagttt
     6361 aatacactag aaactaatta tactttatat gtaggaaata gacaaaatat gatagtggaa
     6421 ccaaattatg atttagatga ttctggagat atatcttcaa ctgttatcaa tttctctcaa
     6481 aagtatcttt atggaataga cagttgtgtt aataaagttg taatttcacc aaatatttat
     6541 acagatgaaa taaatataac gcctgtatat gaaacaaata atacttatcc agaagttatt
     6601 gtattagatg caaattatat aaatgaaaaa ataaatgtta atatcaatga tctatctata
     6661 cgatatgtat ggagtaatga tggtaatgat tttattctta tgtcaactag tgaagaaaat
     6721 aaggtgtcac aagttaaaat aagattcgtt aatgttttta aagataagac tttggcaaat
     6781 aagctatctt ttaactttag tgataaacaa gatgtacctg taagtgaaat aatcttatca
     6841 tttacacctt catattatga ggatggattg attggctatg atttgggtct agtttcttta
     6901 tataatgaga aattttatat taataacttt ggaatgatgg tatctggatt aatatatatt
     6961 aatgattcat tatattattt taaaccacca gtaaataatt tgataactgg atttgtgact
     7021 gtaggcgatg ataaatacta ctttaatcca attaatggtg gagctgcttc aattggagag
     7081 acaataattg atgacaaaaa ttattatttc aaccaaagtg gagtgttaca aacaggtgta
     7141 tttagtacag aagatggatt taaatatttt gccccagcta atacacttga tgaaaaccta
     7201 gaaggagaag caattgattt tactggaaaa ttaattattg acgaaaatat ttattatttt
     7261 gatgataatt atagaggagc tgtagaatgg aaagaattag atggtgaaat gcactatttt
     7321 agcccagaaa caggtaaagc ttttaaaggt ctaaatcaaa taggtgatta taaatactat
     7381 ttcaattctg atggagttat gcaaaaagga tttgttagta taaatgataa taaacactat
     7441 tttgatgatt ctggtgttat gaaagtaggt tacactgaaa tagatggcaa gcatttctac
     7501 tttgctgaaa acggagaaat gcaaatagga gtatttaata cagaagatgg atttaaatat
     7561 tttgctcatc ataatgaaga tttaggaaat gaagaaggtg aagaaatctc atattctggt
     7621 atattaaatt tcaataataa aatttactat tttgatgatt catttacagc tgtagttgga
     7681 tggaaagatt tagaggatgg ttcaaagtat tattttgatg aagatacagc agaagcatat
     7741 ataggtttgt cattaataaa tgatggtcaa tattatttta atgatgatgg aattatgcaa
     7801 gttggatttg tcactataaa tgataaagtc ttctacttct ctgactctgg aattatagaa
     7861 tctggagtac aaaacataga tgacaattat ttctatatag atgataatgg tatagttcaa
     7921 attggtgtat ttgatacttc agatggatat aaatattttg cacctgctaa tactgtaaat
     7981 gataatattt acggacaagc agttgaatat agtggtttag ttagagttgg ggaagatgta
     8041 tattattttg gagaaacata tacaattgag actggatgga tatatgatat ggaaaatgaa
     8101 agtgataaat attatttcaa tccagaaact aaaaaagcat gcaaaggtat taatttaatt
     8161 gatgatataa aatattattt tgatgagaag ggcataatga gaacgggtct tatatcattt
     8221 gaaaataata attattactt taatgagaat ggtgaaatgc aatttggtta tataaatata
     8281 gaagataaga tgttctattt tggtgaagat ggtgtcatgc agattggagt atttaataca
     8341 ccagatggat ttaaatactt tgcacatcaa aatactttgg atgagaattt tgagggagaa
     8401 tcaataaact atactggttg gttagattta gatgaaaaga gatattattt tacagatgaa
     8461 tatattgcag caactggttc agttattatt gatggtgagg agtattattt tgatcctgat
     8521 acagctcaat tagtgattag tgaatagata aaaatatgtt aaatatatcc tcttatactt
     8581 aaatatataa aaataaacaa aatgatacac tacataaagt gttctatcta atatgaagat
     8641 ttaccaataa aaaggtggac tatgatgaat gcacagtagt tcaccttttt atatttctaa
     8701 tggtaacaaa atattttttt atataaacct aggaggcgtt atgaatatga caatatcttt
     8761 tttatcagag catatattta taaagttagt aattttaact atatcatttg atacattatt
     8821 aggatgttta agtgcaataa aaagtcgtaa atttaattct agttttggaa tagatggagg
     8881 aatcagaaaa gtagcaatga tagcatgtat atttttttta tcagtagttg acattcttac
     8941 aaagtttaac tttttattta tgttaccaca agattgtatc aattttttaa gactaaaaca
     9001 tcttggaata tctgaatttt tctctatttt atttatttta tatgaaagtg taagtatatt
     9061 aaaaaatatg tgcttatgtg gattaccagt acctaagaga ttaaaggaaa aaatagcaat
     9121 tttactagat gcaatgacag atgaaatgaa tgctaaggat gaaaagtaag taatggtaga
     9181 tataataaag atattaacaa ataaaaagtg ttatccaaat aagaatagct gaaagttatc
     9241 ataattcatg aaactaataa tgaaaacgag ggagcagatg ccaagagaca cacaagtatt
     9301 aaatacatat aatttcgaag caagtgttca ttactatata gatgacaagg tagtatatca
     9361 aacattggtt cacaaagatg gtgcatggtc agttggtaaa atctattaag ctacattagt
     9421 tacagatatc acaaactata atagttaaac atagaaatat gtgtaaattg tgattgaaat
     9481 tattcaaaaa cacaaaaata cgtgatgaag gacaaaatga tatagaaaat aagtatcaaa
     9541 ccttaataaa tgatttaatt gatagtttaa aagttatagg aaaaatatat aaagaaataa
     9601 aaacattaaa aaaatataag atatgtttac aaattactat cagacaatct ccttatctaa
     9661 tagaagagtc aattaactaa ttgagtatct ttaaattgaa atgttaggaa gtgatttaaa
     9721 tatgaaaact taaattataa aaaatcaata ttaatttatt tttaaaaaat agaaaggagt
     9781 gtataagatt tattttcaaa gtttaaaaac aagaaaatca atttaaattt cagaaggaat
     9841 aaatgtggtt atagaagtgg atttattatc aaaaataata atactaggag gtttttatgt
     9901 ctttaatatc taaagaagag ttaataaaac tcgcatatag cattagacca agagaaaatg
     9961 agtataaaac tatactaact aatttagacg aatataataa gttaactaca aacaataatg
    10021 aaaataaata tttacaatta aaaaaactaa atgaatcaat tgatgttttt atgaataaat
    10081 ataaaacttc aagcagaaat agagcactct ctaatctaaa aaaagatata ttaaaagaag
    10141 taattcttat taaaaattcc aatacaagcc ctgtagaaaa aaatttacat tttgtatgga
    10201 taggtggaga agtcagtgat attgctcttg aatacataaa acaatgggct gatattaatg
    10261 cagaatataa tattaaactg tggtatgata gtgaagcatt cttagtaaat acactaaaaa
    10321 aggctatagt tgaatcttct accactgaag cattacagct actagaggaa gagattcaaa
    10381 atcctcaatt tgataatatg aaattttaca aaaaaaggat ggaatttata tatgatagac
    10441 aaaaaaggtt tataaattat tataaatctc aaatcaataa acctacagta cctacaatag
    10501 atgatattat aaagtctcat ctagtatctg aatataatag agatgaaact gtattagaat
    10561 catatagaac aaattctttg agaaaaataa atagtaatca tgggatagat atcagggcta
    10621 atagtttgtt tacagaacaa gagttattaa atatttatag tcaggagttg ttaaatcgtg
    10681 gaaatttagc tgcagcatct gacatagtaa gattattagc cctaaaaaat tttggcggag
    10741 tatatttaga tgttgatatg cttccaggta ttcactctga tttatttaaa acaatatcta
    10801 gacctagctc tattggacta gaccgttggg aaatgataaa attagaggct attatgaagt
    10861 ataaaaaata tataaataat tatacatcag aaaactttga taaacttgat caacaattaa
    10921 aagataattt taaactcatt atagaaagta aaagtgaaaa atctgagata ttttctaaat
    10981 tagaaaattt aaatgtatct gatcttgaaa ttaaaatagc tttcgcttta ggcagtgtta
    11041 taaatcaagc cttgatatca aaacaaggtt catatcttac taacctagta atagaacaag
    11101 taaaaaatag atatcaattt ttaaaccaac accttaaccc agccatagag tctgataata
    11161 acttcacaga tactactaaa atttttcatg attcattatt taattcagct accgcagaaa
    11221 actctatgtt tttaacaaaa atagcaccat acttacaagt aggttttatg ccagaagctc
    11281 gctccacaat aagtttaagt ggtccaggag cttatgcgtc agcttactat gatttcataa
    11341 atttacaaga aaatactata gaaaaaactt taaaagcatc agatttaata gaatttaaat
    11401 tcccagaaaa taatctatct caattgacag aacaagaaat aaatagtcta tggagctttg
    11461 atcaagcaag tgcaaaatat caatttgaga aatatgtaag agattatact ggtggatctc
    11521 tttctgaaga caatggggta gactttaata aaaatactgc cctcgacaaa aactatttat
    11581 taaataataa aattccatca aacaatgtag aagaagctgg aagtaaaaat tatgttcatt
    11641 atatcataca gttacaagga gatgatataa gttatgaagc aacatgcaat ttattttcta
    11701 aaaatcctaa aaatagtatt attatacaac gaaatatgaa tgaaagtgca aaaagctact
    11761 ttttaagtga tgatggagaa tctattttag aattaaataa atataggata cctgaaagat
    11821 taaaaaataa ggaaaaagta aaagtaacct ttattggaca tggtaaagat gaattcaaca
    11881 caagcgaatt tgctagatta agtgtagatt cactttccaa tgagataagt tcatttttag
    11941 ataccataaa attagatata tcacctaaaa atgtagaagt aaacttactt ggatgtaata
    12001 tgtttagtta tgattttaat gttgaagaaa cttatcctgg gaagttgcta ttaagtatta
    12061 tggacaaaat tacttccact ttacctgatg taaataaaaa ttctattact ataggagcaa
    12121 atcaatatga agtaagaatt aatagtgagg gaagaaaaga acttctggct cactcaggta
    12181 aatggataaa taaagaagaa gctattatga gcgatttatc tagtaaagaa tacatttttt
    12241 ttgattctat agataataag ctaaaagcaa agtccaagaa tattccagga ttagcatcaa
    12301 tatcagaaga tataaaaaca ttattacttg atgcaagtgt tagtcctgat acaaaattta
    12361 ttttaaataa tcttaagctt aatattgaat cttctattgg tgattacatt tattatgaaa
    12421 aattagagcc tgttaaaaat ataattcaca attctataga tgatttaata gatgagttca
    12481 atctacttga aaatgtatct gatgaattat atgaattaaa aaaattaaat aatctagatg
    12541 agaagtattt aatatctttt gaagatatct caaaaaataa ttcaacttac tctgtaagat
    12601 ttattaacaa aagtaatggt gagtcagttt atgtagaaac agaaaaagaa attttttcaa
    12661 aatatagcga acatattaca aaagaaataa gtactataaa gaatagtata attacagatg
    12721 ttaatggtaa tttattggat aatatacagt tagatcatac ttctcaagtt aatacattaa
    12781 acgcagcatt ctttattcaa tcattaatag attatagtag caataaagat gtactgaatg
    12841 atttaagtac ctcagttaag gttcaacttt atgctcaact atttagtaca ggtttaaata
    12901 ctatatatga ctctatccaa ttagtaaatt taatatcaaa tgcagtaaat gatactataa
    12961 atgtactacc tacaataaca gaggggatac ctattgtatc tactatatta gacggaataa
    13021 acttaggtgc agcaattaag gaattactag acgaacatga cccattacta aaaaaagaat
    13081 tagaagctaa ggtgggtgtt ttagcaataa atatgtcatt atctatagct gcaactgtag
    13141 cttcaattgt tggaataggt gctgaagtta ctattttctt attacctata gctggtatat
    13201 ctgcaggaat accttcatta gttaataatg aattaatatt gcatgataag gcaacttcag
    13261 tggtaaacta ttttaatcat ttgtctgaat ctaaaaaata tggccctctt aaaacagaag
    13321 atgataaaat tttagttcct attgatgatt tagtaatatc agaaatagat tttaataata
    13381 attcgataaa actaggaaca tgtaatatat tagcaatgga ggggggatca ggacacacag
    13441 tgactggtaa tatagatcac tttttctcat ctccatctat aagttctcat attccttcat
    13501 tatcaattta ttctgcaata ggtatagaaa cagaaaatct agatttttca aaaaaaataa
    13561 tgatgttacc taatgctcct tcaagagtgt tttggtggga aactggagca gttccaggtt
    13621 taagatcatt ggaaaatgac ggaactagat tacttgattc aataagagat ttatacccag
    13681 gtaaatttta ctggagattc tatgcttttt tcgattatgc aataactaca ttaaaaccag
    13741 tttatgaaga cactaatatt aaaattaaac tagataaaga tactagaaac ttcataatgc
    13801 caactataac tactaacgaa attagaaaca aattatctta ttcatttgat ggagcaggag
    13861 gaacttactc tttattatta tcttcatatc caatatcaac gaatataaat ttatctaaag
    13921 atgatttatg gatatttaat attgataatg aagtaagaga aatatctata gaaaatggta
    13981 ctattaaaaa aggaaagtta ataaaagatg ttttaagtaa aattgatata aataaaaata
    14041 aacttattat aggcaatcaa acaatagatt tttcaggcga tatagataat aaagatagat
    14101 atatattctt gacttgtgag ttagatgata aaattagttt aataatagaa ataaatcttg
    14161 ttgcaaaatc ttatagtttg ttattgtctg gggataaaaa ttatttgata tccaatttat
    14221 ctaatactat tgagaaaatc aatactttag gcctagatag taaaaatata gcgtacaatt
    14281 acactgatga atctaataat aaatattttg gagctatatc taaaacaagt caaaaaagca
    14341 taatacatta taaaaaagac agtaaaaata tattagaatt ttataatgac agtacattag
    14401 aatttaacag taaagatttt attgctgaag atataaatgt atttatgaaa gatgatatta
    14461 atactataac aggaaaatac tatgttgata ataatactga taaaagtata gatttctcta
    14521 tttctttagt tagtaaaaat caagtaaaag taaatggatt atatttaaat gaatccgtat
    14581 actcatctta ccttgatttt gtgaaaaatt cagatggaca ccataatact tctaatttta
    14641 tgaatttatt tttggacaat ataagtttct ggaaattgtt tgggtttgaa aatataaatt
    14701 ttgtaatcga taaatacttt acccttgttg gtaaaactaa tcttggatat gtagaattta
    14761 tttgtgacaa taataaaaat atagatatat attttggtga atggaaaaca tcgtcatcta
    14821 aaagcactat atttagcgga aatggtagaa atgttgtagt agagcctata tataatcctg
    14881 atacgggtga agatatatct acttcactag atttttccta tgaacctctc tatggaatag
    14941 atagatatat caataaagta ttgatagcac ctgatttata tacaagttta ataaatatta
    15001 ataccaatta ttattcaaat gagtactacc ctgagattat agttcttaac ccaaatacat
    15061 tccacaaaaa agtaaatata aatttagata gttcttcttt tgagtataaa tggtctacag
    15121 aaggaagtga ctttatttta gttagatact tagaagaaag taataaaaaa atattacaaa
    15181 aaataagaat caaaggtatc ttatctaata ctcaatcatt taataaaatg agtatagatt
    15241 ttaaagatat taaaaaacta tcattaggat atataatgag taattttaaa tcatttaatt
    15301 ctgaaaatga attagataga gatcatttag gatttaaaat aatagataat aaaacttatt
    15361 actatgatga agatagtaaa ttagttaaag gattaatcaa tataaataat tcattattct
    15421 attttgatcc tatagaattt aacttagtaa ctggatggca aactatcaat ggtaaaaaat
    15481 attattttga tataaatact ggagcagctt taactagtta taaaattatt aatggtaaac
    15541 acttttattt taataatgat ggtgtgatgc agttgggagt atttaaagga cctgatggat
    15601 ttgaatattt tgcacctgcc aatactcaaa ataataacat agaaggtcag gctatagttt
    15661 atcaaagtaa attcttaact ttgaatggca aaaaatatta ttttgataat gactcaaaag
    15721 cagtcactgg atggagaatt attaacaatg agaaatatta ctttaatcct aataatgcta
    15781 ttgctgcagt cggattgcaa gtaattgaca ataataagta ttatttcaat cctgacactg
    15841 ctatcatctc aaaaggttgg cagactgtta atggtagtag atactacttt gatactgata
    15901 ccgctattgc ctttaatggt tataaaacta ttgatggtaa acacttttat tttgatagtg
    15961 attgtgtagt gaaaataggt gtgtttagta cctctaatgg atttgaatat tttgcacctg
    16021 ctaatactta taataataac atagaaggtc aggctatagt ttatcaaagt aaattcttaa
    16081 ctttgaatgg taaaaaatat tactttgata ataactcaaa agcagttacc ggatggcaaa
    16141 ctattgatag taaaaaatat tactttaata ctaacactgc tgaagcagct actggatggc
    16201 aaactattga tggtaaaaaa tattacttta atactaacac tgctgaagca gctactggat
    16261 ggcaaactat tgatggtaaa aaatattact ttaatactaa cactgctata gcttcaactg
    16321 gttatacaat tattaatggt aaacattttt attttaatac tgatggtatt atgcagatag
    16381 gagtgtttaa aggacctaat ggatttgaat attttgcacc tgctaatacg gatgctaaca
    16441 acatagaagg tcaagctata ctttaccaaa atgaattctt aactttgaat ggtaaaaaat
    16501 attactttgg tagtgactca aaagcagtta ctggatggag aattattaac aataagaaat
    16561 attactttaa tcctaataat gctattgctg caattcatct atgcactata aataatgaca
    16621 agtattactt tagttatgat ggaattcttc aaaatggata tattactatt gaaagaaata
    16681 atttctattt tgatgctaat aatgaatcta aaatggtaac aggagtattt aaaggaccta
    16741 atggatttga gtattttgca cctgctaata ctcacaataa taacatagaa ggtcaggcta
    16801 tagtttacca gaacaaattc ttaactttga atggcaaaaa atattatttt gataatgact
    16861 caaaagcagt tactggatgg caaaccattg atggtaaaaa atattacttt aatcttaaca
    16921 ctgctgaagc agctactgga tggcaaacta ttgatggtaa aaaatattac tttaatctta
    16981 acactgctga agcagctact ggatggcaaa ctattgatgg taaaaaatat tactttaata
    17041 ctaacacttt catagcctca actggttata caagtattaa tggtaaacat ttttatttta
    17101 atactgatgg tattatgcag ataggagtgt ttaaaggacc taatggattt gaatactttg
    17161 cacctgctaa tactcataat aataacatag aaggtcaagc tatactttac caaaataaat
    17221 tcttaacttt gaatggtaaa aaatattact ttggtagtga ctcaaaagca gttaccggat
    17281 tgcgaactat tgatggtaaa aaatattact ttaatactaa cactgctgtt gcagttactg
    17341 gatggcaaac tattaatggt aaaaaatact actttaatac taacacttct atagcttcaa
    17401 ctggttatac aattattagt ggtaaacatt tttattttaa tactgatggt attatgcaga
    17461 taggagtgtt taaaggacct gatggatttg aatactttgc acctgctaat acagatgcta
    17521 acaatataga aggtcaagct atacgttatc aaaatagatt cctatattta catgacaata
    17581 tatattattt tggtaataat tcaaaagcgg ctactggttg ggtaactatt gatggtaata
    17641 gatattactt cgagcctaat acagctatgg gtgcgaatgg ttataaaact attgataata
    17701 aaaattttta ctttagaaat ggtttacctc agataggagt gtttaaaggg tctaatggat
    17761 ttgaatactt tgcacctgct aatacggatg ctaacaatat agaaggtcaa gctatacgtt
    17821 atcaaaatag attcctacat ttacttggaa aaatatatta ctttggtaat aattcaaaag
    17881 cagttactgg atggcaaact attaatggta aagtatatta ctttatgcct gatactgcta
    17941 tggctgcagc tggtggactt ttcgagattg atggtgttat atatttcttt ggtgttgatg
    18001 gagtaaaagc ccctgggata tatggctaaa atatatgttt gataaaaaat tattcctgtg
    18061 ctactaagaa attattttta tataataaat attgagattt aattaaaagt catgtgttat
    18121 tgtaatacat gacttttagt taaaattttt ctatcattta ataatctatt attcttgact
    18181 attttataat aaaattcata tatggaaata ttaatactaa ataattaata gttgataaaa
    18241 aatagataat atgctaaaag caaaaactaa tttagagcct tgtaactgtt tatttgcaat
    18301 tataaaaaca tctttaaaca tattgactat aatataaaat attaactata atacaaaaca
    18361 atattaatta attttctcta cagctatccc tggtatagtt atatttccac ccatagttga
    18421 ttcataagtt atagttccag cacttatacc tcttatagtt atgtaatcat cctctaatac
    18481 tcttgaagga gttattgatt ttttataact acatagtaca accttatcat aatttccaga
    18541 cacagctaat cttatttgca cctcatcacc atcttcaata acttgaataa ccttaccttc
    18601 aaattttacc tttttgtact tataatcatc aggtgttcta gctaattggt cataagtaat
    18661 accagtatca tatcctttct tctcctcttc ttcttttttg cgttgttctt cagccttttt
    18721 agcttcttca gcttttttag cttcttcagc tttacgttga ttttcagctt caatagcttt
    18781 cttttcgtcg tctttcattt tgaaccatgg ttcagcatca gacaatttgc tatttaaagt
    18841 ttctatttct gttttttgag aatctataac tgatgtttgg ttagaaatga cctcctcatg
    18901 gtcttcagaa caagctggtg aggatatatt gccaaaaaac acgcctaaaa taaatgccag
    18961 tagagcaata ccctttgtgc tcttaaagaa tttaattatt ttcttagagc ttccttttcc
    19021 ttcattacta aattcgttac cctcattttt tttagaaaac ataatacaat ctccctttta
    19081 ttagatatat tttattttaa aagttaaaaa cttaaaataa ctagttgact aaaaattata
    19141 tattcatatt ttcctaaaat atatttcccc taagcataat tttacaaaat aaagtaagaa
    19201 gattcaatat attttgaaaa aataagataa aaatgtcaga ttataatata attttcttga
    19261 aggtgaattt tatatattaa attaaacata aagtatttta tttgtataaa actatattat
    19321 gatgtaatta agtgactcag tgatttagag gtggtaaaat atatatatat gaaagagcat
    19381 aaataaattg atataataaa aaattaaata ttaaatatta actataataa aaaaatatct
    19441 ggtaataggt ataatataaa aagaatatac cagaaagaag gtgaagtatg ggagaactac
    19501 aaaaaattcc aggggtaggg aaagcaacag aaaaatcttt aatcatgtta ggatatacaa
    19561 ctattaaatc acttaaagat gcaaacccag cacaaatgta tgaaaaggaa tgtttaatgc
    19621 gtggacagca catagacaga tgtcagttat atgtatacag atgtgcagta tattttgcat
    19681 caactgaaaa tccagaacca gaaaaattaa agtggtggta ttggaaagat taaaaaatca
    19741 aaaaagttag acataaaaaa gagttgaata tccgttttaa ataataaaaa ctaatactca
    19801 actctttttt agtcgtaaat tttatgtaaa tctatttgta tggctaaata tacccatcaa
    19861 taaatgtata aatatactaa tcaacaaatg actaaacata ctacctaaca aaggattaaa
    19921 taaattcctt aacaaagcat tctaaaacaa ttaattagaa actgtttctt caagccattt
    19981 tctacattgc tcaatattta ccatagggtc aaaaccttgg tcttttccct tatttaaaag
    20041 catatcacaa gggaaagtat cacattcacc acaatgatta agttccttac tttcacagca
    20101 agttttaacc ttacattctc caccccaaaa tggtttttcc atagttgggc aaccagtgca
    20161 atttacttgt tctttttttg tacaactgtc acaacagaca ccacatcttg attcaaacat
    20221 attcaaacat cctttccatt atttaaaaat attataaaat gattttagat gaatacaaaa
    20281 gtgcaaatag ttgattttag gtcatctaaa tgttaaacat aaaattatta gtactggtaa
    20341 atttataaaa actattccaa aaatcttttt gaatatttca aaacacaata aattacttca
    20401 aatccccttg aatatatttt acagcattat agattgcttt aaatctcttt gaatattcta
    20461 caagactata gattagttta aatccctttg aatatatttt acaacattat agattgtttt
    20521 aaatctcttt gaatattcta caagactata gattagttta aatccctttg aatatttcaa
    20581 aacaccatag attactttaa atccctttga atgttctata acactataaa ttacttttaa
    20641 cttttatata accataaagt ttttatctgg cactttaaat ggagttttat atcaaatttg
    20701 ccttccatcg aataaccaaa cttaatttaa ttacacattc caagagtcaa atattcattc
    20761 aatatatttg ataattctat gttatagcta taattaaata tagattatta aagttaaata
    20821 tttatttaat atattgaatg attttttacc tatatttaat taagtttcat tatataatat
    20881 aaaaattgtt tttgcattaa gttttaatat aatacataat aattttaaat aataacatta
    20941 ttatatattt ttaaatttta ggagtatatc tattatatag cgagtagata aaacatataa
    21001 aattcaaata aaagatatat atttttaaat aaaatggata tagatttcaa ctaaaaagaa
    21061 tataaatttc aaacccaaaa atatctagca tttaccatta agaaataaca tcattctttc
    21121 taaaatttct attggcaatt tcagaaaata ctatagtagt aataaatgca acacatattg
    21181 taactatagt gtcattaaca tttaaagcag attgctttat aacccccata gcactaccta
    21241 acatagaata agggaaaaat ataccaacag caggttttgc tactattaat cccataccaa
    21301 atagagaaag gcaaagacta ataccaatag gagttgcaaa acttctaatc tttatagaca
    21361 tgtataattg tatagaagct attgaaatag aaataatcca acctttagca atccaaccag
    21421 tcaattcttt aggtacacta ccagaaaaac caaacatatt tcctgcaact atataaaaga
    21481 taacaaagaa aacttgtgta atagctgtaa gtattgaaac tactgtaagt ttagataaaa
    21541 agatattttt taaattaact ggcagtgtca ttacattatt ccaattatga ttcatatgtt
    21601 caagcctaca aatatatgca caaaagattg cgattaagat aggaaagaaa aattccccat
    21661 aaaaaagact aacttgagtc cataagctgt accattcttt tttgagtatg ccttgattta
    21721 gataaaaatt accagaacca atcaacacac ttataagtgg caaagctaat aatacatacc
    21781 atataaaaga attttttaac tttagaattt ctcccaaaac tgacttcctt aacatttaaa
    21841 tccctcctat acttcttttt ttaataaatg atttcttcca atataataaa ttagcattcc
    21901 aattaagaaa actaaaatcg taatagaaaa attcatatca attctataga cagatgaacc
    21961 taaaacatta tcataagcat aacttaaagg tgataactct aggtaataag accatataaa
    22021 caatctacga ataccttgat aaaacaaacc tgatgtcaaa cctataaaac taccaatcat
    22081 gccaaatgta attgcaatca tttgatttgg aattacagtt gcaatccata ggtgtagtgc
    22141 aatgatagca atacttacaa gaattgttcc taatgtgtat tttaaaagta aagatacaga
    22201 aattgactca ataacccctc taaaatgtcc aaacaacaca atagataaca cttgcatcaa
    22261 tacaggtata gataccaaaa tagcagaaca taaaaattta gataaataaa tactatttaa
    22321 agatgtcaca gaagatttta aaagcttcca tgtatctcct ttatgttcta tatcgcttat
    22381 acgtgatgta attattgata ttaagatagg aaagaataga ccatttaaac ttgcaaaact
    22441 cataagcata taggaatatt caaatccacc taacatatct ggattacgtg taatagctct
    22501 atttgaattt acgatacacc acattatttc aacagctgca aataaaaagg tcatcagaat
    22561 aatcttcttt cttttaagtt tataaaattc tagaccaagg tatttcatta taaagtcacc
    22621 gccttaccag taagttctaa gaatatatct tccaagcttt ttttatgttc ttctattcta
    22681 aatacatcta tatttgaatg gacaagcata ccatttattt tagcgacatc tgagtctttt
    22741 aaattttcaa tagtcaagta cccatcttgt ataagtgaca tatatccttt agtatttaat
    22801 agtttttgtg cagatacaac attcttagtt ttgatagcaa tctgactttg acctctatta
    22861 tgaagttctt ttatgctatc ttgaaaaacc agttcgccat gatttattat tcctattgat
    22921 gtggcaattt ggtcaatttc acttaaaaga tggctagaga ttagaacagt catcccgtat
    22981 ttttctggta gagattttat taattctcta atttcctcaa tccctgctgg gtcgagtcca
    23041 tttgtaggtt catcaagtat taataatttg ggaaacttca ttattgccat agcaatgcca
    23101 agacgttgct tcatacctaa agaatattga ctaacttttt tatttttttg attttctaac
    23161 cttacaattt ttaatgcttc atttacattt tttttaggta aatctaagag tctttgcatt
    23221 atggtcatgt tttcaagccc tgtaagatga ccatagtatg atggagattc tatcaaagaa
    23281 cctatatctt ttaaaattga tattctattt ttttcattta actctttacc taaaatatta
    23341 atatttcctt gtgtgggttt agcaagtcca agaagcattt taagagtggt agattttcca
    23401 gctccattag gacccaaaaa accatataca gctccttgtg gaactcttag gtctatatcc
    23461 tttactctaa atattttatt gtattccttt gaaagtcttt gtgttctaat tatcatttcc
    23521 atatgtttca tccccttttt taatctgtgc ttattgattc ttttaactta tatcagtata
    23581 ataacttggt tacatttcaa ttagctgaaa tttaccttac aataacctta aaagtctatt
    23641 taggcaaagt tgtgaaaatg ggtatatggt atactaaagg agggtgatga ttatggagtc
    23701 aataaaagat aaaaaaatac ttgtggttga tgaccacaag gaattattaa aaatgataga
    23761 tgaaatcctg agaaaagagg gatttagtag agtattttta gcatcaagtt atgaggaagc
    23821 tgttagggta tttagaaatg taaagccaga ttgtgcaatt ttagatgttg tattgccaga
    23881 tggagatggt ttttctatta tgagaaaaat tagagagaca tctaaaatac cagtgatttt
    23941 tctctctgca agaggtgaag atgaagatag attgattggt ttgggcttag gggctgacga
    24001 ttatatagta aagccatttt taccaaagga acttacttta aggcttgtgg gtatattaaa
    24061 tcgtgtatat gtgcctatag aagaagagga gttgccagta tttaaactgg gagattctgt
    24121 tgttgtgaat ttaaatagtg catgtgttga gaaagatggg caagagattt ctttaacagc
    24181 taaagagcat tcgttgcttt taaagttgta tgaaaataaa gggagaattg ttactaatga
    24241 ttcactatgt caagccattt ggggagatga tatgtatgga tatgaaaata cccttatggt
    24301 tcatgttaga agggttagag agaagataga aaaaactcca tctacaccta aacatcttat
    24361 tacagtaaga ggtcttggat ataagttgat gattaattaa ttttagatat tagaataaaa
    24421 gaaatcaaga ggagtgattt aattttggat agtgtaaagt acatattgag gcgatttata
    24481 ggttcaagta tcttgatttc attacttttt attgtattaa atatagcagg ttttcttctg
    24541 ttttcatata tgttatttgt atctaaatac tctgataatt tagatacaag tgatagtaga
    24601 gaaataaaat caaacttgat tatgattgaa gataatttga ttttaagaga tggaaagttt
    24661 attttagatg attctagtaa ggaagtacta cataaaaata aagtttgggc aatgttaatt
    24721 gataacaatg gagatagaat atggggattt gatttaccaa aagaaattcc aagtcattat
    24781 actttgacag atgtagcaaa gttttcaagg ttctttttaa aggactatcc tgtgtatgta
    24841 tgggaacatc caaatggaat attagtaaca ggttatccaa aagacaagta tactaagttt
    24901 aaccttactt atacggttaa ggaaatagaa caatttccta tgttaataat aggtatgttt
    24961 ctggttaatg caatagttac ttttttaata gctctattca taggtttaaa aatggtgaaa
    25021 tctataaaac caattattac tggcataaag cttatgtcaa aaggagaacc agtactttta
    25081 caagaaaaag gaatactaag tgatatttca aaatctatta atactgtatc taaagagttg
    25141 caaatgaaag atgaaaaact taggaagaaa gaggaagcta ggtctaactg gattgcagga
    25201 atatctcatg atataagaac tccactttca atgataattg gatatgctgg agaacttgaa
    25261 gaaagcagta gcctttcaaa tagagaacaa gagcaagtgt ctattatatg caatcaaggg
    25321 attaaaatca gagaattagt aaatgaccta aatttagtgt ctaaacttga atataatatg
    25381 caaacattga attgtgataa gattagagtt tctgcgtttt tgagagagct tatttctgag
    25441 tttataaata ataatttgga taatgacttt tacgttgaac ttgatatact aaatgaagat
    25501 attattatag atgcagataa aaaattatta aaaagggcta ttagtaattt aatacaaaat
    25561 agtataactc ataaccaaca aggatgtaat ataagggtta cctctaagtc tgatttagat
    25621 ttttgctata taattgttga agatgatgga aaaggtatga gtcaagaaag aattaatagt
    25681 ttattaaaga gccaaaatgg actaagtgag gcatatgcca aaggtcaaaa tcatggtcta
    25741 ggtattttga tagtatctgg cattattaat gcacatagag gtaaattaga tattacgagc
    25801 attgatagtc agggactaaa aacaatacta aaattacctc taattaagtg atattttata
    25861 aaataataac agataagaat gaagaatgaa aatattttga aaataatttt attctttatt
    25921 gacaaattaa ctctattaga gtagaataag gataaattct tttcttgtca agttttatta
    25981 aagtaaagag taacttgtgc tttatgcgct tatggctgag ttactcatgg tatcaaaatt
    26041 ttcatagaaa taatattttg g
//
LOCUS       DAYAOY010000052        25922 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYAOY010000052 DAYAOY010000000
VERSION     DAYAOY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.052"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..396
                     /locus_tag="ACGI6B_002270"
     CDS             <1..396
                     /locus_tag="ACGI6B_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFG9751712.1"
                     /translation="LVYGEKIGYFIFNLVGLDEIFIKIWDILRYTVGIITIIVIFTLL
                     YKYTPNKKLTIKESAPGAIFATFAWFLVSFLYSYYTNYYANYEVIYGSIAEIIVLMTW
                     MYFSSWSIVIGYEVNSRLYFRKIRHEMLK"
     gene            913..2769
                     /locus_tag="ACGI6B_002271"
     CDS             913..2769
                     /locus_tag="ACGI6B_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9751713.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2852..3092
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    3158..3393
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3478..4695
                     /locus_tag="ACGI6B_002272"
     CDS             3478..4695
                     /locus_tag="ACGI6B_002272"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFG9751714.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4812..5294
                     /gene="cdeM"
                     /locus_tag="ACGI6B_002273"
     CDS             4812..5294
                     /gene="cdeM"
                     /locus_tag="ACGI6B_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HFG9751715.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            5558..6823
                     /gene="hisD"
                     /locus_tag="ACGI6B_002274"
     CDS             5558..6823
                     /gene="hisD"
                     /locus_tag="ACGI6B_002274"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFG9751716.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(7026..7778)
                     /locus_tag="ACGI6B_002275"
     CDS             complement(7026..7778)
                     /locus_tag="ACGI6B_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9751717.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7896..8165)
                     /locus_tag="ACGI6B_002276"
     CDS             complement(7896..8165)
                     /locus_tag="ACGI6B_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFG9751718.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            8640..9734
                     /locus_tag="ACGI6B_002277"
     CDS             8640..9734
                     /locus_tag="ACGI6B_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9751719.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            9736..10686
                     /locus_tag="ACGI6B_002278"
     CDS             9736..10686
                     /locus_tag="ACGI6B_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFG9751720.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            10679..11863
                     /locus_tag="ACGI6B_002279"
     CDS             10679..11863
                     /locus_tag="ACGI6B_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751721.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11876..13372
                     /locus_tag="ACGI6B_002280"
     CDS             11876..13372
                     /locus_tag="ACGI6B_002280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFG9751722.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            13395..14345
                     /locus_tag="ACGI6B_002281"
     CDS             13395..14345
                     /locus_tag="ACGI6B_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751723.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            14366..15328
                     /locus_tag="ACGI6B_002282"
     CDS             14366..15328
                     /locus_tag="ACGI6B_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9751724.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            15700..16332
                     /locus_tag="ACGI6B_002283"
     CDS             15700..16332
                     /locus_tag="ACGI6B_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9751725.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            16758..16835
                     /gene="kdpF"
                     /locus_tag="ACGI6B_002284"
     CDS             16758..16835
                     /gene="kdpF"
                     /locus_tag="ACGI6B_002284"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFG9751726.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            16882..18558
                     /gene="kdpA"
                     /locus_tag="ACGI6B_002285"
     CDS             16882..18558
                     /gene="kdpA"
                     /locus_tag="ACGI6B_002285"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFG9751727.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            18583..20640
                     /gene="kdpB"
                     /locus_tag="ACGI6B_002286"
     CDS             18583..20640
                     /gene="kdpB"
                     /locus_tag="ACGI6B_002286"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFG9751728.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            20659..21285
                     /locus_tag="ACGI6B_002287"
     CDS             20659..21285
                     /locus_tag="ACGI6B_002287"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFG9751729.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            21522..22430
                     /gene="cysK"
                     /locus_tag="ACGI6B_002288"
     CDS             21522..22430
                     /gene="cysK"
                     /locus_tag="ACGI6B_002288"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFG9751730.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            22457..23047
                     /gene="epsC"
                     /locus_tag="ACGI6B_002289"
     CDS             22457..23047
                     /gene="epsC"
                     /locus_tag="ACGI6B_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFG9751731.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            23207..23395
                     /locus_tag="ACGI6B_002290"
     CDS             23207..23395
                     /locus_tag="ACGI6B_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFG9751732.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(23449..24318)
                     /locus_tag="ACGI6B_002291"
     CDS             complement(23449..24318)
                     /locus_tag="ACGI6B_002291"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFG9751733.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(24515..25759)
                     /locus_tag="ACGI6B_002292"
     CDS             complement(24515..25759)
                     /locus_tag="ACGI6B_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751734.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9990 a         2744 c         4631 g         8557 t
ORIGIN      
        1 ttagtttatg gggaaaaaat aggttatttt atttttaatc ttgtaggatt ggatgagata
       61 tttataaaaa tatgggatat tctaagatac actgtaggta taataacaat aatagtgata
      121 tttacacttt tatataaata cactccaaat aaaaagttaa ctattaaaga atctgcgcca
      181 ggtgctattt ttgctacttt tgcttggttt ttagtatcat ttttatattc ttattataca
      241 aattactatg ctaactatga agtaatctat ggaagtatag ctgaaataat tgtacttatg
      301 acctggatgt attttagtag ttggtctatc gttattggct atgaggtaaa ttcaagattg
      361 tattttagaa agataagaca tgaaatgtta aagtaaagta tttaaaataa aagagagtat
      421 ttagtattat aaatgtaatt gaattaataa agaaatattg acatttaagt taaaaagtct
      481 agaatttttc tgggcttttt ttatttttag tataacttga caaattttat ttaatgggat
      541 agttttaatt tataatcatc tttaaaaaga tgactattga taagtaagag ataaaattgg
      601 taagtatcaa aaaatatact aaataaaaat tatagtccta atgagttaat ttctgtattt
      661 tcaaataatg cagtatttag tcatgtgttt ttatatgcgc ctgtatttct aattaccaat
      721 atattagaat aagctactgt aattagattt agaaaaaagc gacataaata attataaaca
      781 aataataaat tacataaaat taaatttatt cacaagtata tcttataaaa tatgttataa
      841 ttaatagaaa attgtcatat aattaattaa gtagtattat tataaataac attgaaaggt
      901 agtggttaga atatggatac ccataataaa tatgtaaatt ttataaaaaa tatacctgta
      961 ccttttttat attgtagaat tgtaaagaga caagaagata tagaatatcg agtagaatat
     1021 ataagtaagg gtatgggcaa agtattacaa ttagaagaag gtatttgtga taaaaatata
     1081 ctagatgtat tgcctgtatt taagtctaaa aaatatttca aagagttgtt ctcaaatgaa
     1141 gtggattgta taaaaaggta cattccgaca cttaaaaatt ggataaatat aaaaaaacag
     1201 ataatagggg attcatacat aattttgtat tttggtaaaa ttgtatttga ttatagacag
     1261 ataatcgatt cctttgataa aaaggaaaaa gtagcttata ttaaagacga agaaggcaca
     1321 tatatagatt gcagtgaaaa tttaatacca atattaaata acaacattaa aacaacgaaa
     1381 gatatctttg gtaaaaatga tatagaagta tggggagaga atacaggaaa attatttaga
     1441 gatgattata gagaaggagt atctagtaaa aaaagatttt tacaaaatct ttttgaatat
     1501 gaagaaacat tttttatggt tgaaaaatat tttttgtatg atgaagacga acttttaggt
     1561 actataggga tagtagacaa tataatatat agtggttaca gtaatagaaa ttataactca
     1621 aaagatttaa tgaagatgat agaacactca attccagaga atatgtttta taaagatgtg
     1681 tatggaaatt atattggatt taattcaggt tttttgaatt tagcttgtat gaataaagaa
     1741 gaattattag ggaagaatag ttataaaata tcagaagaag aagctttaat agataagata
     1801 tttgaaagtg ataagggtgt agttgaaaat aaaaaagtag ttacatttga gttaaatata
     1861 agtatgaatg atgaaaacaa gtgtattgag attacaaaaa ggccgttttt tgatagctat
     1921 ggaagtgtta ttggaataat tggaacagca agggatataa gtagaagaaa aagattagaa
     1981 gaggaaatgg ataaaactag gatggaattt tttgcaaatt tatcccatga acttagaacg
     2041 cctataaact taatatcatc ttcattacaa gtgatagaaa aaaaagaagc agatttaata
     2101 gaatcaaatg atactttaaa aagaaattta ggtataataa aacaaaatgg aaatagaata
     2161 ttaagattag taaataatgt tatagatttt acaaaaatgc aatcaggata tttagatttt
     2221 aaaccagaag aatctgatat aattgcattt atagaggaga tatgtatgtc tgtagctgat
     2281 tttgcaagcc aaaataatat acaacttaca tttgatacag aaatagaaga attttcaatg
     2341 ttatttgatt cagaaaaatt ggaaagaata atattaaatt tactttctaa tggaattaag
     2401 tacaataaga aagatggaaa aataaatata tttttatatg tgaaagataa tgtatttaat
     2461 atgaaaatat ctgatagtgg aataggaata ccaaaagaaa aaatagataa aatatttaat
     2521 aggtttgaac agattgacaa tgaactttct tacagagtta aaggaagtgg tattggactt
     2581 tcattagtga aatctttggt tgagttgcat gagggaagta tatctttaaa aagtcaactt
     2641 ggtattggaa gtgaatttat agtttcattg cctgttagaa gtaaaaataa tattgaaaaa
     2701 tataatcata aaagagaaat cagcaatgaa ttaagtaaaa aattagaaat agagttctcg
     2761 gatttataag tttaaatata aaaaataaaa ttttaaaaaa gtattgacaa ttgatttaga
     2821 agggtttaaa ataaattaca acataataaa taaaaaggcg atgaatagga agagtaaatc
     2881 taattcgatt accagagaga agagtccgtt ggctggaaga ctttttaaat tagagggatt
     2941 gaaaactacc taggagctac tttctgaagt catctgcatt agtagatttt agtaagaata
     3001 gtcgttagct gcgttaaagt gtaaagtgag ttttcatgac atatgaaaac taaattaggt
     3061 ggaaccgcgg gaataatact ctcgtcctta tgttataagg atgagagttt tttgttattt
     3121 aaaaatataa acagtaatca taataattat agaaaatata aaagcaatga ataggaagag
     3181 taaatttaat tcgactatca gagaggaaag ttcacaggct gaaagacttt ctaagtttga
     3241 gaaattgaaa actacctagg agctgtggtc tgaagttact ttaaaaagtt ttagtaagac
     3301 taaccgttaa ctgcgttaaa gtgtaaagtg gattttatca ataaatgata aaatcaaatt
     3361 gggtggaacc gcgagtatag actcgtccct ttatataggg atgagtctat ttttattgca
     3421 ataaaagatt gaaaataaaa aaaaatcgga aaattataaa tttaggagga aaataaaatg
     3481 gagaataaag tgaaaattgg agtactagga tatggagttg taggaagtgg acttatagat
     3541 ataatagata ataataaaga aaaaagaaat atagaaatag ttggaatttt agttaataat
     3601 ttggaaaaac ataaggataa aaaatattct aatataataa ctaataatat agatgatata
     3661 tttaataaag atatagatat acttgttgaa gtcatgggtg gtttagaacc atcattatct
     3721 tatataaaaa aagcattaaa caataaaatt catgttgtta cagcaaataa agatttgtta
     3781 gctgaatgtg gagatgagct tgcaaaatta gcaagtgaaa ataaagtaag tattaaattt
     3841 gaagcttcag ttgcaggagg tataccagta ttaaaaccaa taatagaatc actagaagga
     3901 aataatatag atagtataaa tgcaatttta aatggaacta ctaattttat attatctaaa
     3961 atgtatgatg agaatctatc ttatgatatg gcattaagac aagcacaaga attaggtttt
     4021 gctgaagcaa atccagagtc agatgtatta gggtatgatg ctgctagaaa actatcaata
     4081 ttatcaactc tagcttatga caatagagta tattggaaag atgtgtattt agaaggaatt
     4141 acagatatag atgaaaaaga tattgaatat gcaaaaaaat taaattgtaa aataaaattg
     4201 attggtcaaa gtaagtatga aaatgataaa gtaagtgctt ttgttagacc tgttttagta
     4261 gaaaaagata atatccttgc tagaatagac aatgagttca atgctgtcat agtgaatgga
     4321 gattcagtag gagaagtttc ttttgtaggt aaaggagctg gaagtttagc aacaggaagt
     4381 gcggtttatt cagatgttat agatataata gataatagag tttcaagtat agattctttt
     4441 actaaagata aaatacaagt taataaaata gttagagaaa agtgtggagc acttcttaga
     4501 tttaaaaaat gtaataaaga tgaaattcta aatatagttg agaattgttt agtaaaattc
     4561 gatattttaa atgatgatga tgaacttgct attatggttt atgctgattc tgaatatgaa
     4621 ataaacaaca gtttatgtct aataaaagat aaaggatact gcgaaaaaat gaataaaatg
     4681 ttgaaaataa gttaaaaaag aaaaaatata agtttatatg taacatattt tgacgatttt
     4741 aatactatat agtatgagat gaaacaaaac atctcataaa aaaattaaaa ttaaagggag
     4801 agatatgtta tatggaaaat aaaaaatgtt attcagaaga ttggtatgaa agaggagaat
     4861 ctacagctaa atggttccaa aatgatagag aagaatatga aagagaagca tatgatgaag
     4921 atagagaaag aagaggttca aactgtggat gttcagattc aggagaaaat agacctagaa
     4981 actgtgaaag atttagaaga gaagctgaga taagagaaag agaagcaaga gaagcattct
     5041 gtgaatcttc agagaaaaag aaagaggcat tagcatatga atgtgaagct agaaaattat
     5101 gggaagaagc agaaaaatac tgggatgaat attcaaaata caactataaa ggaatcgaat
     5161 atttagcaga agctgctaga ttatttgatg aaggtatgga atgtgaagct agaagaaatg
     5221 gaaataatgg aggaaacaat aataattgtt gccataaatg ccataaatgt aattgtaact
     5281 gctgtagaaa ataaaaaaat taattgacaa atcaatagtt aaaatatatc atattatgta
     5341 taataaagag aaaattcaga aaaaaataat gattagaaga aatataagta tatttattag
     5401 acaaatagtg ctcagttagt gaggtgagta caatatgatt aaactcccat cctctagctt
     5461 ttgatagagg gtgggagttt ttatttattt atatatctta aatgaggata atattgtaaa
     5521 ataaatgagt atgagattgt aaaattagga ggtaaaaatg aaaagtatta aaacatcaag
     5581 tatgaaagaa aaggtagatg aaaagatatt aagagagcaa gtaagttcta taatagaaga
     5641 tataagaaat aataaggatg ttgcacttaa aaaatataat gagaagtttg atagaaatac
     5701 aagagatgaa tttagaatta ctaaggaaga aataaaggaa gcttataagc atgtagatga
     5761 cgaatttata aataacttaa agattgctgc caaaaatata agagaatttg ctaaagctca
     5821 aaaactaagt tttgaaaacc cttttgaaaa agaaatttat ccaggtgtaa tcttaggtca
     5881 aactaatatt ccaatagagt catgtcttgc atatgttcca ggaggtcagt atccattgtt
     5941 ttcaacagct cttatgctta taatacctgc aaaagtagca ggagtaaaaa gaatagtggc
     6001 atgttcacca actatgaaaa atactgaaaa gataaatcct aagacattgg tagctatgga
     6061 tatagcaggt gctgatgaaa tatatgcaac tggaggagtt caagctatag cagcatttac
     6121 atatggaaca gaaaagatta atccagttga cataattgta ggaccaggaa ataagtttgt
     6181 aactgaagct aaaagacaat gttatggaca agtagggatt gattttgtcg ctgggccaag
     6241 tgaagttctt ataatagcgg atgaaacttc aaatccagtt tatattgcag ctgatttatt
     6301 agcacaatgt gagcatgatt taaatgcaag aggaatatta ttgacaaata gcttggaagt
     6361 agcacaaaaa gttgaaaaaa atatagaaag tatgctaaaa gatttgccaa caaaggacat
     6421 agcatatagt tcatgggaaa ataatggaga gataatattg gttgatgata tggaagaagc
     6481 cattaaaata agtaattttt atgcaccaga acatttagaa gttgcagtaa atgagtataa
     6541 tgatatttgt gatagattaa ctaactatgg ttctttattt attggaaatc tttctgctga
     6601 ggtgtttggt gactatgttt caggtaccaa tcatacatta ccaacactta aagcatcaag
     6661 atatactggt ggtgtttggg ttggaacttt tatcaaaact tgtacaaagc agatatttaa
     6721 tgaaaaagct atacaatcat tagcacctgt agctgaaaag ctggcaaaag aagagggatt
     6781 atatgcacat gcaaaggctg ctgaggttag gtttaaaaag tagtaaaaaa ttttattttg
     6841 gcagtcgaga cttagtatta gactgagata aggaaaacgc tgagaacctg aataaatact
     6901 atattttgtg ggtctcggcg cttcttttat atctgtatat aatagaataa tactatctgt
     6961 gtgtttaaat actaacttaa tatttctttt ctaaataatg tttatgaaat agttaatatt
     7021 tttagttaat acttgaaaaa aacttttgct aactgtgcaa attcatctgc atttgtcaat
     7081 atagcagggt gtcctccctg actaaaaatg taaatacttg cttttgaaat aaaagttgcc
     7141 atttgcttgt actcttcttc aagattatga cgacacattt cgtcttcctt acttcctgta
     7201 aataaaattg gcatttctaa tttacacaat tctttatgaa acaaagggcg tttctcgttt
     7261 gcacattgca atagagcttt tgtatcaaga ttaacaacat tctcccaatc ttttccatga
     7321 caccattcat agaattttcg tgcttgaata tcttgctttg atttttccct tccagtgatt
     7381 agattatcta tgaagttatc attaagagta cgtccatcaa agctatctgc tataattgct
     7441 tcaactaaat cgggacgttc taatccagta ttaacagctg cccatgctcc tccacttgtt
     7501 ccaattaagc caactttacc acaattcaaa tgttcaatca gtgctattgt ctgtaaggct
     7561 tcatcatgcc atatgtcagg agaaaattga gaaactctat ccgactgtcc attacctaaa
     7621 aaatcaatta agatacatct aaaattttct gcatataatg gcacaagtgc tttaaacata
     7681 ttagaagaac cagtattacc atgcaggaaa attatcggtt taccttgtcc atattcttta
     7741 taaaaacaag aattaccttg atactgaaaa tatgacatat attttcctcc tttttatttg
     7801 ttaagttaat tataacaata taggtatgaa tgaaaaacct catgttcttt cttagaataa
     7861 aaatgtgcca cgcataaaac gtgacacatt tctaattata aattttgctg tttaagtatt
     7921 ctttgtatgc ttttctctgt caaaaagtat ttctctgata actgcttgat actcattcca
     7981 ttttggaaat ttatacagat ttgagaatta cgagaagcta agacttgttt ggtgtttgtg
     8041 ttttcacccc aatgcttttt gtgctcttgc tttttgggaa tataaatcac tttaccatct
     8101 atatattctt gcacttgctc aatcagttct ttaggcagta agtcaatcga attttgatag
     8161 ctcattttgc acctcctaaa taattattct ttaggattag caaaggctac tgccactcac
     8221 aattaaaaat aaaatttttt attatgtaca atagcctttg ctatgcacaa gttccaattc
     8281 ccataaacta aatactcctt tctggtttga aaaattcatc tgaacattga tttcgctttt
     8341 aagatagagt tcaatgacaa atttacattt ttattatata tattcagata gataattgca
     8401 actgtctttg tatgaggata ttttattata gtcaatacta taatatgtat ttatatcatc
     8461 aaactaatat gaacagttga aaggaatatg aacaataaaa cttagtcagg aagaataatt
     8521 atgaagcaga caatagagaa atttgatttt aaagttttat tgaaaattta ttgacaaatt
     8581 taacttgtaa atattatact aaacttaata aataaagttt ataaagtaag gtgttgtcta
     8641 tgaaaaatat agttggaaaa ccgcaaacga taaaaaaagt aaatgaagat ttaataaaaa
     8701 acattataaa aaatgaaggt cctataacaa aaccagaaat ttcaagcatt actgaattga
     8761 gtttagctac agttaataag attgtagagc aattagcttt gaaaaatgaa gttaaagtaa
     8821 gtggactaag tgaatctact ggaggaagaa gagctcaatt atttgaaata aatgcaaact
     8881 tagaacacat aatagcttta tattattata gaaattgttg tataggggta gttgccaatt
     8941 tattaggtga aataatttat caagaagaat ttaatataag aatggatacg tatgaaaacg
     9001 ttaaccaaga tacttttttt gttatagata ctctaataaa aaaggaaaaa ggaaacaata
     9061 taagggctat tggaattggt gtcccaggag ttgtaaaaaa aggtgtagtt aaaaatatac
     9121 caactataaa aagttgggaa ggtataaatt tagaaaattt aataaagagt aaatacaata
     9181 ctaaagtgtt tatagaaaat gatacaaata ttactactat tgggatttat caaaaacagt
     9241 ataaagacaa atataaaaat atggctctta tgtatttaga agatggtatt ggttcaggta
     9301 tagttataaa tggtgaattg tatttaggtt ctacaaattt tgctggagaa ttaagttatc
     9361 taaatataga atttggtaag tataactcta acaacttaaa tcttgaagat tatattatga
     9421 gtttaagaaa aagatattta gaaagtaaag ataataaatt taagaaagaa attttatcta
     9481 taatatcaaa ctttataagg actcttgtat gtgtcctaaa tccacaagct atgattatac
     9541 agtgtagtat attaaccaag agtgacatgg agtttataga aaaagatgta agcaataatg
     9601 ttggagaaga aaattttcct aaattaataa aagtagactc attgagagaa ggaagtataa
     9661 atggagtaat tagtatgtgt ttaaaagaaa ctgactatca atattcctta tctaataaaa
     9721 aaggaggata ttaatgtgaa atatttggtt tcaatagatg gagggggaac aaaaactaaa
     9781 ttttgtgtaa gtgacttaga tggaaacata cttaaagaac ataccactgg ttcaactaat
     9841 tataagtcag ttggaattaa aaaaacatat gaaaatatta ataatggatt taaaaaaata
     9901 ttaaaagatt tatatatcga ttatgacgat attgagtaca ctgtatttgg tatatctggt
     9961 tgtgattctc ctaatgatta taagattata atggatgaaa tattaaaaat tgggattaac
    10021 aaagaaaaaa tatatctggc aaatgatgca gttttagctt tttatgcaca agctgattcc
    10081 cctggcttag taattgtggc tgggacaggt tcaattatac tgggaattaa agaagatgga
    10141 gaaatatata gagttggtgg atgggggtat aattttagtg atttgggttc tggatatgat
    10201 atagggagaa aattattaaa gaaagtattg ctttattgtg atgaatgtca tgagtatagt
    10261 gatttattta gttgtgtctt ggacttcttt ggagcaaata gttttgaaca acttgcatat
    10321 atgattactg atataaatga taatgttgaa atagcaaact tagcttcatt agtgattgat
    10381 tgtgccgaac aaggtgataa attggctatt gaaatattaa gagaatcatc aactgaacta
    10441 tctaaattag ctcaagtaat attgagaaaa atatctaatc caatgaaaat ggataaaagc
    10501 gaaataaata tagttttgtc tggtggtaca ttaaataaaa ctgtatatgc tcaaatgtta
    10561 atgaataatt taaaaacaga aaatacagat aaaaatttaa aatttatatc tcaagaaaat
    10621 cagccagtat atggggggat aagacttgct atggcattag ctaataggag ggtcaagcat
    10681 ggatagaaaa aaggtaatag tacaaatttt tactggaggt tttaaggcta aagaaataaa
    10741 gtttaatgat ataaaatgta aactactaga attattggac tgtattgata tagaaaaggt
    10801 tatcatggga tggagtgtag atgaagaatt atatgtagaa actaaaaaac tattaaataa
    10861 atataatgtt gaactatatc tttggttgcc atgtttttct gaaataggat tacttgaaaa
    10921 aagtaattta ctaattgatt atatggggaa ggaagtcaag ggctatgctt tacaagagga
    10981 tgaaaatttt gagttttatt gcccaaataa taaggaaaat ataaaaaata taaagaaaat
    11041 atatagtact tattttagtg atattaaatt tgatggaatt tttttagata aaataaggta
    11101 tggttctttt tcgaataaac taaatggtgt ttttaattgt ttttgtaaag attgtttaaa
    11161 tatatataaa aaaaataata ttgataaaga gtcgttaatg agagaaatga ataatgtgtc
    11221 taagggagtt atagataatg aagatactcc atttggtata acagaataca ttaatggaaa
    11281 atacaaattt aaaaataaaa tatgggaaga tttttttgaa acaaaaaatc aaaatatata
    11341 caaggcatta ttagagataa gtagttattt taaaagtaag ggattgaaaa taggtataga
    11401 tgctttttct ccttttattt cttactttac aggtcaagat ttagaaaaat tacaagaagt
    11461 agcagatttt ataaaaccaa tgatgtatag aactacaaaa gctccagctg gtttaccatt
    11521 tgaatttgaa aggtttattg atgaatccat acataagaat aaagagattg gaaaaagaaa
    11581 tttcttagat attttaggaa ttaaggagta tgaaggagat gtatatccta tagaatttgt
    11641 actagaggaa atcgatttta tgacaagaaa tcttaataaa gatatatatg taggagttga
    11701 aataaataaa aaggaaatta tatctcctgt tacacctaag tacataagtg aaaatatttc
    11761 aaatataaat aagacaacag taaaaggata tgttttatct tgggatttgt tgtcagcacc
    11821 aaaagaaaat attgatgaaa ttataaaatg gtttcggaat tagtgaggtg ataaagtgag
    11881 taagatattg gaaatacaac aaatatataa aagttttgga aaggtagatg ttttaaaagg
    11941 gataaatttt gagttagaaa agggagaagt aagagctctt ttgggagcta atggagcagg
    12001 aaaatctaca cttattaaaa ttttaggagg agttcattct caaactaaag gtaaagtaat
    12061 tttgactggt aatgaaataa tgtttaaaaa tccacatcat tctaaagaaa atggtattag
    12121 tataatttat caagaattaa gcttgatacc aacgcttaca gtacttgaaa atatttttct
    12181 tggtagagaa attttaaaag gaagtgtgtt tttaaacaag aaagacatgc ttgaggaata
    12241 tatgaaaatt tgtgaggatt ttgattttaa tatagaccca catatagtag tttctaattt
    12301 aagtgtagca aaccaacaaa tggttgagat aatgaaagct gtttcatgta attctgaaat
    12361 tatcataatg gatgaaccta caacatcact tactaataat gaaaaagatg ggctatttaa
    12421 aataataagt agactaaagt ctaaaggtaa aacaatagtc tatatttctc acatattaga
    12481 ggaagttttt aaagtatgtg atagtgcaag tataatgaaa aatggaatta tgattggaac
    12541 ttatgatgta gaggatttaa ctaaattaaa gataactcaa cttatgacag gaaatgataa
    12601 tcacaaactt attgagaaga aggattatac atttgcaaaa tatgaagagc caccagtact
    12661 agaagtaaag aatataagct ggtctaacaa aataaaaaat gtttcattta aagtttatag
    12721 aggcgaagtt gtaggtcttg caggtcttgt aggttcaaga agaactgaaa ttataaattt
    12781 gatatttggt atagataata aagatagtgg agatattttt atcaatggta aaaaagtaga
    12841 cataacttct cctaaggttg ctataaaaaa caaaatagga ttgattcctg aagatagaaa
    12901 aaatctagga ttaattttag aacaagctat atacacaaac tcatcatcag tacaaattga
    12961 taaattcaag aaatttttgt ttatagatag aaataaagaa attgagtttt ctaaaaaagg
    13021 tgttaaggaa ttaggaataa aagttagtga tgtaaagcaa gaagtaaaag agcttagtgg
    13081 tggaaatcaa caaaaagtag tagtttctaa gtggttagag aaagatttag atttaataat
    13141 atatgatgag ccaacaaagg gaatagatat tgctgcaaaa gaagatattt tcaatacaat
    13201 caaagaattt tcaagtaaag gaataggagt aatatttata tcttctgatt tagaagaagt
    13261 aataagagta agtgatagaa ttttagtaac tagagaaggt tctattgtag atgatttaaa
    13321 aaatgagaat atacaggttc aagatataat gaacagtata tttaatgtat aaaaaatgaa
    13381 aatggaggac tgagatgaag aaagcttttg agaagttaaa tttaagagat tatggagtat
    13441 tattaggttt tttagcttta tgtatagcta taagtattgc aactccatca tttttaggta
    13501 aacaaaatat acttaatctt ttacgacaat cttctataat tggaataatt tcagcaggta
    13561 tgacttttgt tataatatct ggaaactttg atatatcagt tggagcagta gcagctcttg
    13621 caggtgctat aacaatgaaa tttgcgactg gtggaactaa tttgtttgca tccatgttct
    13681 taggtgtagc gatttgtgca gtaataggat taatcaatgg tatagtagtt gcaaagataa
    13741 atgtaccatc attaatagcc actatggcta tggtcactat tgtaagagga ttgctgttga
    13801 tgcttacagg aggatatcca attactgaaa atataccaat acttgattac attggaaatg
    13861 gatatttgtt taatatacca ataccagtag taattttctt tatagtggtg ttagcttcgt
    13921 ttattgtact taataaaact aagtttggta gatatgttta ttctgtagga gggaatcaag
    13981 aagcaagtaa acttaatggt ataaatgtag attctcacaa agtgaaagtg tttattatta
    14041 atgctgtact tgcaggaata gctggaatcg tactaacagg aagacttggg actgcaactg
    14101 cgattgcagg tgaaggatat gatatggatg caattgcatc tgtagtaata ggaggtacat
    14161 cagtggcagg aggttctggt tctgtactta aaactgttat aggagtatta cttatgagtg
    14221 ttataaacaa cagttttaat cttcttggaa tagatgtata tttccagtac atatttaaag
    14281 gattaataat tttagcagca gttggatttg attcttatag caagaaaaaa cttgcttcaa
    14341 gataaaaaat aagggggatt tttttatgaa aaaattaatg aaacatttag cattgttgct
    14401 atcacttgtt atgatatttg gtttagttgg ttgttcaaat ggtggtgaca aagaaaaaaa
    14461 tggagaaaag aaaatagcag tattactacc aggttcaaca ggttactttg tagcaactaa
    14521 acaaggtata gatgcaaaat ctaaagaatt aggtgtaagc gttgaatatg cagatgctca
    14581 atgggatgct agtaaacaat tatcacaagc tgaagatttt atggctaaag gggtagacat
    14641 gattgcatta tgtggagttg attctgctgt aagtgagaga atagttaaag ctgctaatga
    14701 ttcagacgtt cctatagtag catttacaaa ttctatagga agtaacccaa ctggagaatt
    14761 taaaggttta gttacatata taggacaaaa tgaggaagaa acaggagcat taactggaaa
    14821 aattgctaaa aacttattag gagaaactgg tgggaaagct gtattaatag aaggagtacc
    14881 aggaactact ccacaagtat taagaaaaaa aggtcttgaa aaagaattaa aagatagtaa
    14941 tatagaaata gtttataatc aaacttcaaa ttgggagaaa gagcaagctt taaaagtaac
    15001 agaagattta attcaaaaga aaactgattt taatgttata atatgtcaag atgataattc
    15061 tgctacaggt gcaggtcaag ctttaaaaga tgctggtctt aaggataaag ttaaagtcat
    15121 aggtttgggt ggaagtaaag atggattaaa agctataact gatgggttaa tagatggtac
    15181 aacttatatg tctgctgtag aagaaggtgg attagtcata gaaaaggcat ctaaattcct
    15241 taaaggtgaa aaaatagaac ctgttacaca aattaaacaa gttgaagtta acaaagataa
    15301 tatttcagag ttcaagggtg aatggtaaat tgtcatagat aataaataag atttttagag
    15361 ttactttaga aactatataa agtaactctt tttttaattt aatttagtga taaattattg
    15421 aatagtattt ctaaacaaat aaaaaattaa tataaacaaa gacttaatta tactaaatta
    15481 taagtaaaac taatataaac ttatggaaaa aaggaaaacg tctaaagtat tggaattact
    15541 atagtgtagg tgtagtatta aaaaaacata aaaaaacata aaaaaattgt tgaaaaaaat
    15601 atagaaattt tgtataatta aagatttgaa aagtgcggta tacaaagcca attcttttgt
    15661 atataatata tataaattca gaaaaaggaa gggatatgta tggccaggcg gttattttta
    15721 gaaaggattg gacttatatt tttaggttca gcaatattag catttggagt ttataatttt
    15781 tattatttaa acaacataac agaaggtgga gtgttaggta ttttactact gttaaaaaat
    15841 ttattcaata tacaaccagc aatagcaaat gttgtaatag atggtttact acttttagta
    15901 ggatataaat ttttcggtaa aaaattcctt atatattcaa tagtagcttc tataactttt
    15961 tcagtgttat atgacttatt tgaagctata ggacctctag taccacaatc acaaaatatg
    16021 cttttatcaa ctattttggc aggtgtaact gttggaactg gtgtaggtat agtagtaaaa
    16081 gcaggttgtg catcaggagg agacgatgct ttagcattag taatttctaa gactacatct
    16141 ttgaacatag ggcaagttta tttagcaaca gatgttattg tattgttgtt atcattattt
    16201 tatctatcag cgtttgatat cttttattct ttgattgctg ttacaattag tggcaaggta
    16261 attgatttta tttattatca tggtaaaagt ttggatatgg atataagtaa tgatatagtt
    16321 ccagaatgtt aatataaatt aaataacttt agattttatt aggggtggca gatttgccac
    16381 cttttttgtg tctaaatctt tatagaaagt ataatcccat ctaggttttt gcaaatctca
    16441 aatatagaat tatgttggaa tgactgggaa tacgattgct agatgtgcat tttaaaaacc
    16501 caattttata tttatgaaat actaatcatt aatggatgtt tcttaatcct atctttatac
    16561 ctaaaatgat aagataaatg aagtagcaat acaaaactaa aatttgtaaa agagattaaa
    16621 ttaaatggag gagctaaaat gttggatata gttatgatag cattgttatt cataggattt
    16681 gtttccatga agttacttgc tgattggtgt gggaaacaaa ctgaaaaaaa ataatgtaat
    16741 gtatggaggg attacctatg tggtttctgg cagttgtaat tataatgtta ataatatatt
    16801 tggtatatgc gcttattaat cctgaaaaat tttaaacagt ataaggctaa taagtaagtt
    16861 gaattggggg gaacaatttt tatggtgcag atagtaatag tgttagcgat ttttatgatt
    16921 ttagtaatac ctatgggcaa gtatttatat catatagcta ctaatcaaaa aacatttgga
    16981 gatagactat ttgataaagt ggacaatttt atatataagg tatgtagtat agataagaaa
    17041 aaagaaatga actggaaaca gtatgcatta gctttattat ttacaaatgc agttatggta
    17101 tttataggat atatcatact tagaactcaa agtatgcata tttttaatcc aagtggaatt
    17161 aaaagtatgg aacaaggact atcatttaat acaataataa gttttatgac aaatacaaat
    17221 cttcaacatt attcaggtga atcaggactt tcatacttta gtcagatgac agtgataata
    17281 tatatgatgt ttacatcagc tgcaacagga tatgcggctg ctatggcatt tgtaagaggt
    17341 ctagtcggta aaaagaaaac attgggaaat ttttatgtag atttaattag gataacaaca
    17401 agagtacttt taccaggagc tttaattatt gggcttatat tagttacaca aggagtacca
    17461 caaacttttg ctgggacaga aacagtgact actatagaag gaaagcttca agatatagct
    17521 agaggtcctg tggcagctct tgaatcaata aaacatttag gaacaaatgg tggtggattc
    17581 tttggttcaa attcatcaca cccatttgaa aatccaacaa taatatcaaa tatagtagaa
    17641 atattatcta tgatgatatt accaggagct tgtgttgttg catttggtca catgataaag
    17701 aataaaaaac aaggttgggt tgtgtttggc gcaatgtcaa tcatattttt aataggtcta
    17761 gtagtgtgct ttaaagcaga aagtgcagga aatcctattt tatcacaatt agggcttaat
    17821 caaagtatgg gaagtatgga aggtaaggaa gttagatttg gtatagcaca atcatcatta
    17881 ttcacaacag tgactacttc ctttacgaca ggtacagtta ataatatgca tgatacactg
    17941 actcctttag gaggattagt accactttta aatatgatgc taaatgtagt atttggtggt
    18001 aaaggcgtag gtctgatgaa tatgttgatg tacgctataa tagcagtatt cttatgtgga
    18061 ttaatggtag gaagaacacc agagttttta actaagaaaa tcgaaggaaa agaaatgaag
    18121 cttattgctt tacttattat attacatcca cttttaatac taatgttctc tggtctttca
    18181 gtagcaatac cagcagggct tgaaggaatt tcaaatcctg gattccatgg attatcacag
    18241 gtattgtatg agtttgcatc ttcagcagcc aataatggtt caggatttga aggtctagga
    18301 gataacacta tgttttggaa tataacaaca ggtgtagtta tgttctttgg aagatatgtg
    18361 tctattatag tactactagc aatatcaagt ttgttagcat ctaaaaaagc tgtaaatgaa
    18421 agtataggaa cacttagaac ggataatttt acatttacaa tagttttagt acttgttgtt
    18481 ttaattgttg gtgctttaac atttttccca gccttagcgc ttggacctat ttcggagcat
    18541 ctagtgttgt ggcactaaaa aattgggagg gagtaaagtt ttatggaaac taaaaaaaca
    18601 aagtttatta caaaagatat tttcaaaact tccattatag aagcttttaa aaaattaaat
    18661 cctaaataca tgatgaaaaa tccagttatg tttgttgtag aaattggatt tatagtaaca
    18721 ttgatactta caatagtacc tagtttgttt ggtgatgaag gcaataactt aagagcttat
    18781 aatgggattg taacaataat cttatttata acagtccttt ttgcaaattt tgctgaagct
    18841 gtagctgaag gaagaggtaa agctcaagca gatacactta agaaaactag aaaagataca
    18901 gtagcaaggg ttatagatac gttaggaaat gaaaaaatga taagtgcaag tgagctaaaa
    18961 cttggtgata ttgttttagt aaatgctgga gaagttattc caaatgatgg agaagttata
    19021 gagggtatag cttctgtgga tgagtcagca ataactggag aatctgcacc tgtcatgaga
    19081 gaatcaggtg gagatttcgc atctgttaca ggtggtacta cagttgtaag tgactggtta
    19141 aagataagaa taacaacaaa accaggagaa tcgttcttag ataaaatgat agctcttgta
    19201 gaaggtgctt caagacaaaa aacgccaaat gagattgctt taaatacttt attagtaggg
    19261 ttaacactta tatttatggt tgttttaata tctctttatc caatggcaaa ctatgcaggt
    19321 gtaaagattc caatttctac tatgatagct cttttggtat gtttaatacc aactactata
    19381 ggtggattgt tgtcagctat tggtattgca ggtatggata gagtaactag atttaatgta
    19441 attgcaatgt caggaaaagc agtagaagct tgtggagacg ttgatactat gattttggat
    19501 aagacaggta cgattactta tgggaataga cttgcatcag aatttatagc agttggaaat
    19561 gcagattcaa aagatttaat aaattattct gttatgtgtt cattaaaaga tgatacacca
    19621 gaaggaaaat ctatagttga tttaggtatg aaattaggtt caacaggtaa gacaaaagag
    19681 gcagaggaag ctgagtttgt agaattttct gcacaaacta gaatgagtgg tattaactta
    19741 gctgatggta gagctataag aaaaggtgct tatgatagca taataaaaag agtaaaagaa
    19801 tctggtggta cagtgccaga agatttagaa gaaaatgtaa atcgagtagc taaactagga
    19861 ggaacacctt tagtagtgtg tgtagataat gagatatatg gaattatcta cttaaaagat
    19921 acagtaaaat cagggcttgt agaaagattt gctagacttc gagagatagg aataaagaca
    19981 ataatgtgta caggggataa tcccttgaca gcagctacaa ttgcaagaga agctggagta
    20041 gatggattta ttgcagaatg taaaccagaa gataaaatag atgcaataaa aaaagaacaa
    20101 ttagagggta aaatagttgc aatgacaggt gatggaacaa atgatgctcc tgcacttgct
    20161 caggcagatg taggtcttgc aatgaatagt ggtacaacat ctgcaaaaga agcagcaaat
    20221 atggtagatt tagattcaga cccaactaag atattggaag ttgttgaaat aggaaaacag
    20281 cttttaataa ctcgtggggc tttgacaaca tttagtatag caaatgatgt tgcaaaatat
    20341 tttgcgatta taccagctat atttactctt gcaatacctc aaatggagat attaaatgta
    20401 atgaaacttt ctacgtcatt tagtgcgata ttatcagctt taatatttaa tgctataatt
    20461 atcccatgtt taatacctct tgcaatgaag ggagtaaaat atagacctat gaaatcacaa
    20521 gctatgttaa tgagaaatat gcttatattt ggtcttggtg gagttatagt accattttta
    20581 ggaataaaat taattgattt attgattaca cctttagtaa tggttcttaa tttaggttaa
    20641 taaatggagg atataaaaat gaaaacatta aagccagcat tacttgtaag tattgtattg
    20701 ttagttgttt gtggacttgt atatccactt gttttgacag gtgtaagtca agttgcattt
    20761 aaagataaag ccaatggaag tatgatagaa gtaaatggtg taaaagtagg ttcagaactt
    20821 attggtcaaa gctttacaga tgcgagattc tttaagggaa gagtatcatc agtaaactat
    20881 aatacttata caaaagaaga tttagtacca gataaagatg gaaatacatc ttatggaggt
    20941 gtatcatcag ggtcttttaa ttatggagct acaaatccag aacttcatga tagagtacaa
    21001 aaagatatag aaaagttttt aaaggataat ccaactgtca aaagagagga tataccaaca
    21061 gatttactta cagcatcagg ttcaggactt gaccctaata taagtccagc ctctgcaaaa
    21121 attcagatac cagctatagc aaaagcgtct ggaatatcag aaagtaaatt acaaaaaatt
    21181 gtagatgata atacttccaa aaaacttttt ggagttttag gtgaagatag agttaatgta
    21241 ttaaaagtta atgtagaggt cgcaaaaata ttaggtctta tatagtattt tgtggaattt
    21301 tagcaacaaa atattacgca agatttaatt taaaggttaa attttaaaga gtgaaaatcg
    21361 ataatgattt tcactttttt atttgaatat tactaatatt tggaataaag tgattgtaag
    21421 tataaaatcc aaaaaaaggt tgcattatca aaatgataat actataatat atttatcgtt
    21481 atcaatttga taatagattt ggacatatag gaggaaatag tatgttatat aataacgcat
    21541 tagagttgat aggaaacaca cctgtagtaa ggctgaataa tttaggctgt actaatgggt
    21601 gttctaacat atatgtaaaa ttagaaaaag taaatccagc aggaagtata aaagatagag
    21661 ctgtttatga aatgttagag ggcttagagc aaagaggaga attaaaaaag ggagatgtac
    21721 tagttgaagc aactagtgga aatactggta ttgcattatc tatgatagga aaattaaaag
    21781 gatatgaagt agtaatcgta atgcccgaaa caatgagcgt tgagagaaga gatttaatga
    21841 aggcttatgg agcaaatttg gtgcttacag atggtaaagc tggaatggca ggttccatag
    21901 aaaaagcaaa tgaactttta aaagaaaatc caaactataa aagcctaaaa caatttgaaa
    21961 ataaagataa tccaaatgca cattataaaa caacagcagt tgaaattatg aatgatgtca
    22021 aagatttaga tatattcgta tgtggagttg gaacaggtgg tactttaata ggaactgcaa
    22081 gatatttaaa agaacaaaat ccaaatataa gagtaattgc acttgaacca aaaaaatctc
    22141 cagctatatc agaaaacaag actggacctc ataaaataca aggaataggt gctaactttg
    22201 ttcctgaaaa ttatgattct agtgtagttg atgaagtaat attagtagat gatgaagatg
    22261 catttgatac agttaaatta cttgcagcaa aagagggaat tttagtaggg atatcttctg
    22321 gagcaaatat atttggtgct atagagatgg caaaaaagta tccagataaa aagatagtta
    22381 cagtagctcc agatggagtt gataagtata tgtctatggg aatattttaa taattttatt
    22441 tgagggagtg aatactttgt ttaagaaaat aaataaagat attgaatata taatgaagaa
    22501 tgatccagca gctaggtcta agatagaagt ttttttactt tatccatcag ttcatgcaat
    22561 gataatgcat aggatggctc atgctcttta taagaagaag aagcttttta cagcaagatt
    22621 aatatcccag atatccagat ttatgactgg catagaaata catcctggtg ctaaaatggg
    22681 ggaaggtata ttgatagacc atggaatggg tgttgtaatt ggagagacag ctgaagtagg
    22741 gaatagggtg acaatatatc aaggagctac tttaggagct acaggaaaag atactggaaa
    22801 gagacatcca actgttggtg atgatgtatt aattggtgct ggaacaaaaa tactaggacc
    22861 tttaaatata ggttcaaatt caaaaattgg tgcaaattct gttgtcgtta aggatgtacc
    22921 taatggtgca acagttgttg gtataccagc aaaaatagta aaaataagaa acctagaacc
    22981 tgtaaaaaag aataaaaaag aagtgtccta tgagtatgat gaattagata atgtatatta
    23041 tatataggat ttatataact ataaaataaa ctttataaaa attattttaa aaggtatttt
    23101 tttcaaattt gaggacataa taaaaaagaa ctgaatgtta cagttttggt tcatcaatct
    23161 ttgataaaca agattttaaa tttgtagcgt ctaggaggat tttattatga aagcaaatgt
    23221 taatcaagat acttgtatag gttgtggatt atgtccatca atatgtccag aagtatttga
    23281 tatgaaggat gatgggaaat cacatgttat agttgatgaa gtaccaagtg atgctgaaga
    23341 atcagcagca gaagctagag aaagctgtcc agttgatgca atagatgtgc attaataaaa
    23401 taaatgccta taaaaattga attttcaatt tttataggca tttgtctttt attcagttag
    23461 tatttcaaca cctgtttctg taactaaaac catacattcc cattgagcag ataatttacc
    23521 atcttttgta taaatagtcc aattattttc ttcatcaaca aaaatttcgt gagttccttc
    23581 atttaccata ggttctattg tgaatatcat cccaggaacc aatagcatct cagtcccttt
    23641 tttagataca taacttacaa aagggtcttc atgaaagctt aatccaactc catgaccacc
    23701 tatttctcta acaacagagt atccattttt ctttgcataa gagttgatag catcacctat
    23761 atctcctaaa aatccccatg gtttggcaac ttcaagacct ttttggacac attcctttgt
    23821 aacttgaact aatttttttg cattttcatt aacattacct atcataaaca ttcttgaagc
    23881 atctgaatag tatccattaa aaatagtaga aacatctaca tttataatat ctccatcttt
    23941 taagataatg tctttacttg gaataccatg acaaacttca ttatttactg atgtacatac
    24001 acttttagga aatccattat agtctagtgg agcagggata gctccatttt gtagtgtaaa
    24061 gtcatgaacc aacttattta tatcttctgt agacattcca atttcgattt tatctgaaat
    24121 ataatccaaa atttttgtgt ttattacagc actttttttt atttcctcaa tttgactttc
    24181 attttttata atatcatgag ttggtacaat atgacctaac ttagcaaatg aatctatttt
    24241 attatccata tccatatgac attttttata ttttttagta cttccacacc aacagctatc
    24301 atttctattt aacttcataa cttttcatcc ttcttttatt atttatttaa attatgggtt
    24361 acataaacta ttttaaaaca taaccattat aaatattata acataaatac taagaaatcc
    24421 tttatagata gtgatttatt caaagtataa attattatat ggttattgtt gatgaaaaga
    24481 ggatagttaa atgtattatt ggatattagc gcacttaata gtttagttca agtttttcaa
    24541 gaaaatcata tgttagagga caattttcct ttaaagtatt ttttgaatct tcatcaagat
    24601 aataatgtac aaatgattct gcaaaaaatt cttctataaa cttgcttata tatgcatgtg
    24661 caacttgctt agggtcataa agcttatatc tttcctgagc aaatacttcc ataaattctt
    24721 cagaatttga ttttttattt aaaacatttt tatctatagc atgtgcagtt tcatgtaatt
    24781 ctaaattttt tgatgaatgt acatttgcat agctagggtt actataacct attttagcca
    24841 ctatagaatt atttctatat ataccaggta catctttcca agtcttgcct gatcttaccc
    24901 aacatggagg taattgatat tttaaatcac taaattctgg ttcatcagtt agatttgaat
    24961 ttattaattt tatttttata tccttttctt taagtgtctg taaatattta atatttattt
    25021 ttgcaagtct gtttttcata ttttcaactt catctttatc ataattcaag tcatcaggaa
    25081 gtataagcat ttgttcaatt atatctatac tactgtcttt tatattttca gattcttcat
    25141 caaacttata ttgaagagta ttattttcat agatagttat atttgtatta ttttctatag
    25201 aataagagtg aatatcctct aaatctaaaa tattcatttg ttgtaaaata tgttttgttt
    25261 cgccactcca tactactaaa gaaagtgaat taaaattaat tagtggattt tcttttgctt
    25321 ttatatcatt gcttatatct acatcaaata catattcaat tgagtcttct atgttaagac
    25381 catctgtatt ctttttaata tataaatacg cttgttttaa gtctatataa atctcatcta
    25441 aagattcgtc ataaaattct gataaattat tagtaaagag ttcttcactt aaaagttcat
    25501 ttttatcagt tatactttca attaaaatat taatttcttt tatcatatcg taaatactta
    25561 tttttctatt tgctttttca ctagatacat tgcatggcgc aagagcaatg caataaaaat
    25621 ctttttcttt tttataaaag gctttaagtg attttgataa aagtgtaaag tctacaccta
    25681 tctgtaagtt gaccttaagt gagtcatttt gtaataattc agtattattt cttttagctg
    25741 tatttaaatt gttttccata atcattgccc cctttttatt agggtatcat aattttttta
    25801 tatgtgtcaa tttgtttttt tgaaaattat aatttttttg aaaattttat tttttataat
    25861 ttgtgaattt tttattatag aaataaatat gttattatta tgtcaaaatt tagcattaga
    25921 tt
//
LOCUS       DAYAOY010000053        25429 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYAOY010000053 DAYAOY010000000
VERSION     DAYAOY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.053"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(289..1179)
                     /locus_tag="ACGI6B_002293"
     CDS             complement(289..1179)
                     /locus_tag="ACGI6B_002293"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFG9751735.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1209..2474)
                     /locus_tag="ACGI6B_002294"
     CDS             complement(1209..2474)
                     /locus_tag="ACGI6B_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HFG9751736.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2584..2686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2740..5427)
                     /locus_tag="ACGI6B_002295"
     CDS             complement(2740..5427)
                     /locus_tag="ACGI6B_002295"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9751737.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRN
                     AFNLTVLNNLEWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5525..6268)
                     /gene="yaaA"
                     /locus_tag="ACGI6B_002296"
     CDS             complement(5525..6268)
                     /gene="yaaA"
                     /locus_tag="ACGI6B_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFG9751738.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6278..6742)
                     /gene="bcp"
                     /locus_tag="ACGI6B_002297"
     CDS             complement(6278..6742)
                     /gene="bcp"
                     /locus_tag="ACGI6B_002297"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFG9751739.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(7094..8791)
                     /gene="ade"
                     /locus_tag="ACGI6B_002298"
     CDS             complement(7094..8791)
                     /gene="ade"
                     /locus_tag="ACGI6B_002298"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFG9751740.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(9097..10251)
                     /locus_tag="ACGI6B_002299"
     CDS             complement(9097..10251)
                     /locus_tag="ACGI6B_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HFG9751741.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(10496..11335)
                     /locus_tag="ACGI6B_002300"
     CDS             complement(10496..11335)
                     /locus_tag="ACGI6B_002300"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFG9751742.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(11348..11944)
                     /locus_tag="ACGI6B_002301"
     CDS             complement(11348..11944)
                     /locus_tag="ACGI6B_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFG9751743.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(11995..12645)
                     /gene="cmk"
                     /locus_tag="ACGI6B_002302"
     CDS             complement(11995..12645)
                     /gene="cmk"
                     /locus_tag="ACGI6B_002302"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFG9751744.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(12691..13944)
                     /locus_tag="ACGI6B_002303"
     CDS             complement(12691..13944)
                     /locus_tag="ACGI6B_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9751745.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLNTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(13968..14609)
                     /locus_tag="ACGI6B_002304"
     CDS             complement(13968..14609)
                     /locus_tag="ACGI6B_002304"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFG9751746.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(14811..15224)
                     /locus_tag="ACGI6B_002305"
     CDS             complement(14811..15224)
                     /locus_tag="ACGI6B_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HFG9751747.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(15375..15716)
                     /locus_tag="ACGI6B_002306"
     CDS             complement(15375..15716)
                     /locus_tag="ACGI6B_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFG9751748.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            complement(16010..16669)
                     /locus_tag="ACGI6B_002307"
     CDS             complement(16010..16669)
                     /locus_tag="ACGI6B_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9751749.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
     gene            complement(17200..17577)
                     /locus_tag="ACGI6B_002308"
     CDS             complement(17200..17577)
                     /locus_tag="ACGI6B_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9751750.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(17764..18735)
                     /locus_tag="ACGI6B_002309"
     CDS             complement(17764..18735)
                     /locus_tag="ACGI6B_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9751751.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(18769..18966)
                     /locus_tag="ACGI6B_002310"
     CDS             complement(18769..18966)
                     /locus_tag="ACGI6B_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFG9751752.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            complement(19034..20245)
                     /locus_tag="ACGI6B_002311"
     CDS             complement(19034..20245)
                     /locus_tag="ACGI6B_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFG9751753.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            20526..21128
                     /locus_tag="ACGI6B_002312"
     CDS             20526..21128
                     /locus_tag="ACGI6B_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9751754.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            complement(21384..22355)
                     /locus_tag="ACGI6B_002313"
     CDS             complement(21384..22355)
                     /locus_tag="ACGI6B_002313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFG9751755.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(22381..23862)
                     /locus_tag="ACGI6B_002314"
     CDS             complement(22381..23862)
                     /locus_tag="ACGI6B_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFG9751756.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            complement(24198..25184)
                     /locus_tag="ACGI6B_002315"
     CDS             complement(24198..25184)
                     /locus_tag="ACGI6B_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9751757.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
BASE COUNT         8257 a         4309 c         2630 g        10233 t
ORIGIN      
        1 ataaaaaatc tatatatata ttttacactg atttctccct ttttaatctc ttttaaaagt
       61 taaaatacaa atttattgat actataaagg tttagtctcc cattatcgca ccatatataa
      121 aattaaagcc acctctatct acatacacct aaagtgattt tcttaaaata taaaactact
      181 attataaggt caaattttaa aaaaataagg tataaagtat cattaagatt aactttatac
      241 ctataaattt ctatattttt ttagttaaca tttatatact cttaaaattt attgtatcaa
      301 atagtttttt atccaatttc taaacaacaa ttctgaatgt tttttccatt ttacagttgg
      361 ctcttctgaa gggtcatcat taatatagta attttgaggt atagatattt tatctccttt
      421 atttatatcc ctcttatatt ctttatcaag agtgtatttg tcatactcgc tatgacccat
      481 tacgaacaca tctcttgaat ttgtaattat atagggacca gcttctttag aatgactcac
      541 aatgctcaac tctttattct ctttcaaagc ttcaatattt acagtagtat gtcttgaatg
      601 tggagcaaaa aattcatttt caaatccatc tactatttta ctatctttat ctaccttatg
      661 cttaaatact ccaaaacatt tttgacttag tggaagtttt tttacattgt agtatttgta
      721 aagacttgct tgagctgccc aacatatata taatgtagat ttagtttttc tcttagaata
      781 atccataatt tcttctagtt cattccaata atcaacttct tcaaactcca tttgttcaac
      841 tggagctcct gttattataa agccatcaaa atactctcct ttggcttcat caaatgtttt
      901 ataaaattct ttaacatatt cattacaact tcttttactt tctcttgttg aaattttaat
      961 aaattccaca gacacgttga atcctgtttt atctatcctt cttagtaaat ctaattctgt
     1021 atctatcttt ataggcatta gatttagtat agctatctta gtgtctatgt tttcttcata
     1081 ctttaattcc tttttaaaat cttctttata aattacatct attccttcat ctaataattt
     1141 attaataaca ggtaatccct ttggtagtat taaagccata tcttttcact tctctttctc
     1201 ttagaatatt agaaatttaa agcttgattt aaatcagcta ttaaatcctc tacattttct
     1261 attccaacag ataatcttat tagtgaaggc aatactccag atgcaattaa ttgttcttct
     1321 gttaattgtc tatgagtagt tgaagctgga tgtataacac aagttcttac atctgaaaca
     1381 tgtgtaacca aagatgctat ctgtaatttt tccacaaatt ttttggcatt ttctaaccca
     1441 cctcttactc caaatgataa aacaccacta catcctcttg ataaatactt tttggcattc
     1501 tcataatact tgttatcttc aagtcctggg taattaatcc aatctacatt ttcatgtttt
     1561 tctaaaaatc tagctatttt aagtgcattt tcactatgtc tctccattct aagatgtagt
     1621 gtttcaacat ttaaattagt aagatacgca ttgaatgggc ttaaacaatt tccatagtct
     1681 ctaagcaact gaactcttgc tttagttgca tatgcggcat ttccaaattt ttgagtatag
     1741 cttataccat gatatgttgg gtctggctca actaattctg gaaattttcc attatcccag
     1801 ttaaagtttc caccatccac tataattcct ccaacacttg aagcatgccc atctaaatat
     1861 tttgtggtag aatgagtaac tatatttgct ccatacttta aagggttaca aagcactgga
     1921 gatgctaatg aattatcaac aataaatgga acattaattc tcttagctac atttgctatt
     1981 ttttcaaaat ctatgacttc aagagttgga tttgcaagtg tttctgcaaa cacaacctta
     2041 gtattttcct ttgcaagttc tactatctca tcttcacttg aatctaagtc aaacgatatt
     2101 aaatctatac caaatttttt aagactaggt cctagtaaat tgaatgttcc tccatatact
     2161 tttgaagaac aaagtatact atctcctgat ttacatatat ttaaaactgc caacatattt
     2221 gcagattgcc ctgatgatac agctacagaa gatactccac cctctagtaa acttattttt
     2281 tcttcaaaag cttgaatagt aggattgctt attcttgaat atatatgtcc atcaactttt
     2341 aaatcaaata attcagcaag ttggtctata ctgctatatt taaaagttgt actttgatat
     2401 aaaggtagta ctcttggttc tccattacct ggtttataat ttccttgcac acatattgtt
     2461 tctttattat acattcttgt atccccctta gattttagta aaaaaaatta aactatttac
     2521 cagatgagag ttaaaataaa taaagcctga gtatataact caggctctgt ataagcttaa
     2581 ttatactcat ctctcagctt tcgccgcagg atttagcacc aatgtatata aatatactgg
     2641 ttgccgggtt tcatagggcc agtccctcca cctctctgga taagatttat ttaatttttc
     2701 tatgttttat atcttatata tttttcctaa ttttgtcaac tattttttta atttttttct
     2761 tttaataatt ttcccaacct ctacttgaat tacagacatt atggctaaaa caatcgttac
     2821 aagccactct aagttattta aaactgttaa gttaaaagca tttctaaaaa caggtacaaa
     2881 tagtattact gctattaaaa atgcagaagt taaaaatgag attaaaagaa atggattttt
     2941 accattattt ctattaaaca ttgaatctgt atttgaacgc tgactaaatg ctcgtaacat
     3001 ctgtgaaaat gctaatacac aaaatgccat tgtttgacct acagaatgat tttctatatt
     3061 aagaccaatt ttatatgcta aaatagtcat tattgccaca aatatacctt gggtaacaac
     3121 tctttgtata agattttttt caaacaaagt ggttgatttc actggctggt gcttcattat
     3181 atttctgctt ggtgggtcaa ctccaagagc aagggctgga agcgtagcag ttgcaaggtt
     3241 tacccataga atatgaacag caagaagcgg agtatcccaa tttaataata ttgctacaaa
     3301 caacgtaagt atctctgcta tattaccaac aagcaaaaat tgtataactt tttgtatgtt
     3361 tctatataca cgtctacctt cttttattgc ataagcaatt gttgtaaaac tatcgtccat
     3421 taaaatcatg tcagaagaat cttttgcaac atctgtacca cttataccca tggctatacc
     3481 aatatcagca gactttaagg caggagagtc atttacacca tccccagtca ttgctggtac
     3541 ttctccaatt ctttttagag attgtataat tctaagttta tcaaatgggg atacacgagc
     3601 aaatactgta gtatttttaa cagcttcatc tagttcatca tcactcaaat tatcaagttc
     3661 atcaccagat atcacagtat tttcttcact ccatattcct aactcatgag cgattgttgt
     3721 ggcagtaact ttatggtcac ctgttatcat tatggttctt atacctgctt ctttacaagt
     3781 acttactgca tcaattacct cagttcttgg tgggtcaatc ataccaacca tacctataaa
     3841 tgttaaatca tattctatat tttcactatc ttcttttggt atagatgaaa tctcacgctt
     3901 agcaaaacca agtacacgca atgcatcttt agacatgtta agacataatt cgtgtatatt
     3961 ctttttatcc acttcagtaa tgtttctttc accttttgaa gttaatattt tagtacatag
     4021 gtcaagaatt tcgtctactg ctcctttagt ataagcaata atcttttcat ctatattatg
     4081 aacagttgtc atacattttc tatctgaatc aaaaggctgt tcaaatacac gtggatattc
     4141 ttcttcaaga gcttcttgat ctattccaaa ttttttggcc ataaaaatta atgcgccctc
     4201 tgttgggtca cctaatattt catctgtttt atctggatta atacatgcat cattacaaag
     4261 tgcaccagca taaacaatct ctttatacac taatggatgt ttttcagatg cagtatctat
     4321 tgatgtcact tttccagatt caaaatctcc attgacagca atatgtgtaa cagtcatttt
     4381 atttaaggtt aatgtaccag ttttatcaca acatattact gtagctcctc ctaatgtttc
     4441 aacagcaggt aattttctaa ttaaagcatt tcttttagcc atacgctgta cgcctaaagc
     4501 cattacaata gtagctgttg caggtaatcc ctctggaata attgatatgg caagggaaat
     4561 tgcaatcata aacattggca agagaggtct ctgataaaat gcacctattg caaatataac
     4621 aacgcataca ataataccta tgacacttaa tgtctttcca acagtattaa gtttacgttt
     4681 tataggagta tcaaaatcat cttgtgaatc taaaagatgt gctatgttac ccacttctgt
     4741 atccatacca gttgctacta caacacctac acctctacca tatgtgacta ttgaagatgt
     4801 atacgccata ttgctacggt ctcctaatac acattcttta ggtaagattg tatctgcatc
     4861 tttttctgat ggaacagatt ctcctgttaa agaagcctct tgaattttta aacttgatgt
     4921 ttctattaaa cgaaggtctg ctggaaccat agctccatct tctaaaaaaa caatatctcc
     4981 tactacaaga ttacttgctg gaataacatt ttcttcacca tcacgcataa cacgagctgt
     5041 aggtgaattc atatttttta atgcttctaa tgatgattct gcttttttct cttgaacaat
     5101 tccaataact gcatttactg ttacaattat tagtattaca aaagcttctg taaattcgcc
     5161 aaatatcaaa gataatattg ctgctcctat tagtatcata accatagcat ctgtaatttg
     5221 actccaaagc atttgtaaag ttgttctagg tggttttttt ctaagttcat ttaagccatt
     5281 ctctttaagc cgtttttccg cctcggtatc ggtgagcccc tcctctgatg aatctagact
     5341 ttctaaaatt tcatgcgttg ctcttgaatg ccaaggcatc tgtttgtgat tttccatttt
     5401 ggaagaccac tcctttcttt ttttaattta agaataatta tattttaaaa tcaagttcaa
     5461 ttcaatacac actaagtata ttttacctaa atttaacaat gccatttaat atttaacaaa
     5521 acttctactc ttttacaaat atgtattcac tattgttaga taagttttct ttatacttat
     5581 aaccttctaa atcaaacttt ttcagttctt caatatctct tatcctattt tttacaatgt
     5641 attttgtcat catacctcta gctttctttg cttgtgtaga ttccactttc aatatgccat
     5701 ttttatttac tttaaaagta catgtaatat atgtatcact atctttaata aatttttcta
     5761 tggatttgct gtattcattt gatgaaagat taacgatagc tgttttttct ttgattagtt
     5821 ctttatatat acgatttccc caatattcgt ataaattctt aaactcacct acttgtaatt
     5881 ttgtctgcat ttctaatcta tattcatata tactatcata tggttttaca acaccataca
     5941 atccacttat gattcgaata cagctatttg caaactcttc atctttcctt gtaaaatttt
     6001 ctgcttctat atttttatat tgtattccat cataagttaa gattgcaggg tttcctaatc
     6061 tatcaaattt aatgctttca aatctatttt tatttaatac agctaatttt tcattaattt
     6121 tcattttatt tttaaaatct tctataccaa atgtctttat attttccact atttcttttg
     6181 tgttatctat aaagcaaggc aaactcaaat ctctatcaaa tacttctata ttcttcatat
     6241 ttttagctgg tgataacatt ataatcataa attcttatta ctcctgtttt tccaaatatt
     6301 caagaatctc ttgtgcattt gtgtctggtt ttgctttttc aaagactttt tcaataattc
     6361 cattttcatc tataatatat gtcgcacgta caactcccaa tgcagttttt ccatacattt
     6421 ttttctcttt ccatacatca aaagctttta tagcaactaa gtcagggtca gaaagtaaaa
     6481 taaaaggaag ttcatgcttt tctgcaaatt tttgatgtga ttttatacta tctttactta
     6541 tcccaataac ttgtatatct tcctttttaa atccatcata tgcatttcta aaagcacaag
     6601 cttgacgtgt acatcctggt gtattatctt taggataaaa atatactact acttttttgc
     6661 ctttaaaatc tgacatactt accttatttc catctttatc ttctagtgta aattctggtg
     6721 cttttgttcc tatactcaac ataaatatct ctccttttgt ttttattaat aattttatac
     6781 tatgcttata ttattaccct ataaatctat atataaataa aatattttat tttttatgta
     6841 taaagtttca taaatatata attttcatgt atttatatgt taaaatagac tgaaaatagt
     6901 tttattaaga tatctatgct taaaacaatg ttaaatgata gattaattaa aataaggata
     6961 tctaaaaata aaatttttag ttcatgagat gctctttttt gtatgaaatc aaatatattt
     7021 tcatcatttc aacaataaaa agagacttat ctctattttg agaaagcctc atttattatt
     7081 acttttatac tatctatata aatttaaatt cctcaacatc aaatagacct aaatctgtaa
     7141 gtcttatttg tggaacaact ggaagtgcca taaaagaaag tgtaataaaa gggtctacat
     7201 actctggaac ttccattttt cttgctatct ccaacatatt atcagtaatt tcttgtactt
     7261 cttcagctgt aaaagtacta attaatccag ctacttgaag acttaggtgt gcaagaactt
     7321 ttccatttga tacaattaca taaccacctt gtatttcctt taagtaattt atagctacta
     7381 gaatatcatc atcattatcc cctgcaacta taatattatg agaatcatgg gccacagtag
     7441 tagcaattgc tccatttttt atgccaaatc cttttagtgg agcaattgct acatttccag
     7501 tcattctatg tctctcaaca acacaaagtt ttgaatactc tttatttgga acaaaatatc
     7561 catctgcaca tggtaaagat tcatatacct tatttgtcaa aatttgataa ggtacaatct
     7621 ccattacata atttttttca cttactttta actgtatttt ttctttatta atatatttaa
     7681 acttgactgt attaagcaat tctttatctt taatctcata attgtaatta gaaaacatct
     7741 cttcgtttac caaattacca tctttatata cactatccac ttccattttg tctaaatcat
     7801 tcaatacaac aatatctgcc ttataacccg cacctattgc tccaattttc cttaacccat
     7861 atgcttttgc agaattataa gttgcaactt ttatagccct aacaggttcc atacctaaat
     7921 caatcgcaca ttttatattc catcttatgt gaccttgttt ttctatatca tctaagtgct
     7981 tatcatctgt gcaaaacata aattcttcaa ttggcaagtt atgctttagc ataccactta
     8041 ctatagactt taaatttttt gctgcgcttc cttctcttat taaaattttt aatcctgctc
     8101 tcaatttttc atatacttca tcaaaagtta tacattcatg gtcattttct attcctgcac
     8161 atacatatgt ctgaagtgat ttaccattta tattaggtgc atgtccatct acaactttat
     8221 ttttaaaaag ctctagttta tccaagattt cattttcaga atttataaca tcattaaaac
     8281 acattacctc tcctaaacct aaaattctag gattatttac ataactctcc atgtcttttg
     8341 cactaaattt accaactcca ttagtttcaa aacttgttgc aggtacggat gaaggcacca
     8401 taatatatac attaagtggt atatcttttg tcgattctaa taaaaaatct attgcactaa
     8461 ctccttttac attgaccaac tcatgtgggt cggctatgat agttgtcgta cctgatttta
     8521 ataatttttt cgataattca attggcatta ccattgtaga ttcaatatgc atatgtgcat
     8581 caataaagcc aggtgatata tatttgttac tacaatcaat ttctacattg cccttgtaat
     8641 cgccaattcc aacaatagtg tctcctacta ttgctacatc tcctgttatt aactcttctg
     8701 taaatacatt tatgattgtc ccatttttta gtacaatgtc agctttttct cttccacttg
     8761 aaacatcaca ctgtcttttt aaattaccca tttttacaac tcctcctaag ttttttatat
     8821 caatatactg actgtattac gtattatagt ttaatttatt aatatatatt atatatgagt
     8881 tttgatagaa agtatcactt gaaatttgtt ccataattcc tttaattaaa tagagagaac
     8941 atgaggaata atattatctc ccatgttctc attaatattt aagcaataat ttaattcata
     9001 ttgaattttt tatttttata tttaatcttc actattctta taaataaatt tatgtgcttt
     9061 attatttttt taatacttct ttattcataa ttatatttaa cctaaaaatg cataaactat
     9121 catacctaca agtattgctg gtatagtagt aataatcata gcccataaag aagatttttt
     9181 atccattgct aaaagtggga aaagtgcatc tccatcttga gaaatagagt tggctatcac
     9241 tgcaccaagt ggtatagttc ccttcaaata aaatgacata agcacaattt gtatcccaca
     9301 acctggaata agtccaagac ctgcacctat aaatacactt attacacctg ttgataatac
     9361 taatttaact aagtattcat ctccacctaa aattaatatt atcaaatcgt aaataagata
     9421 tgcaataaaa atccatgtaa ttacaaatga tatttctcca acagaatgga taagcatttc
     9481 tcgcaaagaa atctttttat tttcttcctc tttttgatta gaatttttaa ataccttttt
     9541 aaaacaccac atataaatta ttgaaaataa tattccaatc actgctatta cttcttccaa
     9601 tgaatgtaac ttctcaatta taggaagatt aagtccagag tgagcaagtg acataaatat
     9661 aaaaccgatt actataattc ctatataaat tttatatccc tttccatgtg ttattataaa
     9721 tgcaagtcta tttgtatttc catgagattt agctagagtg tctaaaatta tttcatcatg
     9781 attatgttgt tctttatttt ctttagggtt atttttactt atatcatatt tatctgattt
     9841 tctacggttt tttaacccta atttttcttc tagtttaaag taatccacta attgtccagt
     9901 tattattcct gtaattgtac taactatagt gacaaaaaaa tacattttaa tatttgatga
     9961 aataagtaca aatgctgcat ctcccatact agctattaaa cttgcaatta ttgctccaaa
    10021 gcttaatttt ccatttatat acaatggcat tattgccaaa cttccgccac aacctggtat
    10081 agctccaatt aaagcaccta ttaagggttg aaattttctg ttttttgata tgatattagt
    10141 aaaatttcca ctagttttat aattaatata tccaaataac aaaataaaaa atccaatcat
    10201 agaacctaca tgtaaaaacg cctcttcaga agcggatatc aacatttcca ttaatttacc
    10261 tcctagacta attttgcaac aattatatta tattctattt tctattgata atcaatatca
    10321 atatttcttt tgaatttcta atattaaaaa gtgcgcatct tatactctta gtttttcatc
    10381 ttaagataca cactttttga tattaggtta tctatttacc acatgtaata tgaaatcaaa
    10441 ggtggatatt atgataaatt ataacaatgt ccacctattt ttattaccta tcaaattata
    10501 aattttcaag gaatgttaca acttcttcta ttatccaatt aggtgtagat gcccctgctg
    10561 taatacccaa atttttatat tttttaagcg tatttacatc caattcatct ttagtctcta
    10621 ttgcaaaagt tggtactaag tcttcacaaa cctttactaa cttttgagta tttgaactgt
    10681 gtttacctcc tataactatc atacaatcaa cttctttagc taactcttta gctgattctt
    10741 gtcttgtttt tgtagctgaa caaattgtat cattaaatac tatttcttct aatttatctg
    10801 atagtttatt aactatactt atatacaact ctggatttat tgtagtctgt gcaacaagac
    10861 aatatctttt ggagttatca aattccatat tttccatttg ttctaaggtc tttattatta
    10921 tagctgaatt ttcacaccat ccatttatac ctataacttc tggatgcttc atatctccaa
    10981 taactattat ttcatagcct ttattatgat attcacttac tactgtatgt attttcttta
    11041 caaatggaca tgtagtatcc acaattttta attttttatc tttggcttcc ttatatatat
    11101 tttcaggaac tccatgagac cttattatca tattttcatg atttggtatt gtatcaattt
    11161 cattcacagt cttaagacct ttttcttcat attttgctac tgcttgcttg ttatgaataa
    11221 gaggacctaa agcatatatg ccagaatcat ttttttcaac ttcatcccat gccattttca
    11281 ttgctctttt tactccaaaa caaaagccag cattttttgc tatttttatg ttcataactt
    11341 taccccctta ttttgaatat ttaaagtaat tttgttttat cacttctaaa atctcttgtg
    11401 atatcctttc atggtcatca ttactcaatt tttccttaaa atgttcttca aatgatattg
    11461 gttctccaat atatacaatt actttactaa aaagtttata tttagatatt attgatactg
    11521 gaacaacttt agatttactt tttattgtaa atacagaaag acctgcctta gccttaccaa
    11581 aagaatctcc ttttattcta gtaccctctg gaaatattcc taatacataa ccgtctctaa
    11641 ctgaccttaa aatagttttt attgtagata catctggttt ttctctattt ataggcataa
    11701 catgcaattt ttttaatata aatcccaatg gttttacttt aaacaattct ttctttgcaa
    11761 ttgcagctat ttctctattt tctatggcag cagccaagaa tatagggtca agattcgatt
    11821 tatgatttga tgctattact atatttcctc tatctggaat attctctgca cctataactt
    11881 catatttaaa aaatatttta gaaaaaccct taaaaatatt tattacaaat ctataaaaat
    11941 tcacgtttat ctcccttcaa cactacattt attttcatta tgatgcataa aaaactaaag
    12001 atttatttta gatacaactt cttctacaac ttcttctata gtttttcctg tagtatctat
    12061 ttctagtgca tcattagctt ttaccaatgg tgcaaattct ctatttgaat caatttcatc
    12121 tcgtcttatt atatcctcaa ttacttcttc taaagtagta ttatatcctt tattacaaag
    12181 ttctttatag cgtctatttc ctctttcttc tggagtagct actaaaaaga atttgtaatc
    12241 tgcatctggg aaaacatatg aacctatatc tctaccatct agtattactg aatttttcat
    12301 tcctattttt cgttgaactt caaccatcaa ctgtcttact tcttttattt ttgctacatt
    12361 agaaacatta ttgcttactt ctattgttct tatttcttca tttataactt ctttgtccaa
    12421 atatatattg ttatctttaa aatctatatc cgaattttca gctatttgaa taacttcttt
    12481 ttcattattg acatcaatac cactttttaa acacttataa gtaactgctc tgtacatagc
    12541 acctgtatct atataattta tatttagctt ttttgctact atcttggcta ttgtactttt
    12601 tccagctcct gctggaccat ctacagctat aactaaatta cccatatata cacctctatt
    12661 tattcatatt taattattaa aaaattatat ttatacactt atacccgcaa gatatccagt
    12721 tgaaaatgct atttgtaaat tatatccacc agtttctgca tctatatcaa ttacttcacc
    12781 tgtaaaaaat aagttcttaa ctaatttaga ttccatagta gaagaattaa tttctttaac
    12841 tgaaatacca ccagaagtaa ccataccagt gtttatacct gtagtcccat tacaagttag
    12901 cttcatattt tttatatact caataattct aatttcatct gccttagaca aatcacttgc
    12961 ctttttatca actaaagata atagagaaaa aatctctttt aagaaatttt gaggtagtat
    13021 ttgcttaaga ttatttagaa catttttatt tggaaatgct ctgattatac tagagatttc
    13081 atcagtattt aaatttggta agaaatctat atttaattct accttttcct tttcaataat
    13141 cttgttaata taagaagaca ttataagaac acaaggtcct gttatcccaa aatgggcaaa
    13201 tagcatgtct ccagattttg atattttcct tttcttaatc ttacaagata tttccacatt
    13261 cttcatggat attccttgta aatctttaat ccatttttcc tctattgtaa gtggaactaa
    13321 agcaggatat agtttgttta atgtatgtcc atgcttttta agaatcttat acattgaacc
    13381 atctgaaccc gtctttgaat aactcactcc tccagttgat ataataacct tgtctcctag
    13441 tattttatct ccattatcta aaataatccc ttttattaga tactttgact tgtccttatt
    13501 agaatcatct tttagagcaa tttcttcagt tataatatct ataactttct tattatacat
    13561 tatctttaca ttgtgattga gtaaatcttt atttaatact tctattactt ctatagattt
    13621 atcattttta gtgtatactt tgtggtcatt ttcttcttca atcttatatt ccaaatttct
    13681 actttcaaaa tacgaaataa ggtctttatt tgtaaatgta taaaaactac tatagagaaa
    13741 ttttttatta ctgacaactt tatcaaaaaa atcttctatc tctctattat tggtaaaatt
    13801 gcatctaccg ccaccagttg ccctaagttt tcttcctaat tctccatttc tctcaactaa
    13861 tattacttca ttattgtttt ttgaagctga caaagctgcc atcattccag aagctcctcc
    13921 accaactact ataactttac tcataaattt ctccttataa ttgattccta ctccagtgca
    13981 gaattattta tctttacatg attttttata tgacttgtat cattcatttt tactataaca
    14041 ggcccataat ctttaaattc tactatattt aaagcagtat tatcttgctt aattctatat
    14101 atattttcca tacctgattc taaaaatgac aacaacataa ccctcaatgt aattgaatgt
    14161 gtaacaagaa ttatattttt attatcatat ttttcattta gttcctttat aaaagcatct
    14221 actctttcct ttataatctt aagagtttcg ccttctggta tatttactaa gtgtggttca
    14281 tttctccatg ttgcatatat atctgaataa tctttttgaa tttcttttat taaaaggcct
    14341 tcccaaactc cgaatcccat ttctctaagt gcctcagttt tttgaacttc aatattgagc
    14401 ttatctccta aaatttgagc tgtttgcatt gctctcccaa ggtcgctact aaaaatataa
    14461 tcaattgaat acttgccaat atcttcagaa agttcttttg cctgttctat accttgaggc
    14521 gtaaggtcag aattaccatg accttgtgtt tttcccaata tattccaatc cgtttgtcca
    14581 tgtcttacta tataaaaagt atttcccata tgtcctccta atatatgcct attttttatt
    14641 aaatataaat tatcacttct caaaacttaa ttatatcata taatacttta ctttgtttct
    14701 attttataat ctaatctaac atattattta caataaatct catttgtatt tctgcaaatt
    14761 attgtactgt taaagaattt ttctaaattt ataaacatat ttctcttata ttatttatat
    14821 acttcttcaa atctgtattc ttgaattgaa ttgacatact tatctgtatc tactttttct
    14881 aaaaacatat ccaaaggtct tgcaaatatt cctgtatcat catataaggt cttgtataaa
    14941 actaaatcat tagtatcttc ttctttttta tgataaaagt ttccatctaa atccctataa
    15001 acatagatat cttcttctct ttcagtatgt ctaataacta atttatattt atttctgttt
    15061 tggattaaat tttctttacc atatatttca catatgtttt ctatatcatc aatagccttg
    15121 ctaagaccca ttgtagcata atatttccct ttgaaatgtt tatagataca aggatatttt
    15181 aactttcttt cactgatacc taagttattc tttaattcaa gcattttatt ataatctccc
    15241 atatctcgtc cctttctttt aaaaagtatt ttattctgaa ataagttact tgttttatat
    15301 tttatcataa tttaaaattt attaataata caatcaataa ataaagaggc tgtataaaat
    15361 acagcctccc aatattaatc ttcataatca tataattttt ctaatttctt ttttaatttc
    15421 ttttttcttt ctgaacctaa aattcttata aaattattat tcacaaaaat acaattattg
    15481 ctgagataaa attcacactt gtctagattt acaatataac tattactgca tttgaaaaaa
    15541 ttttcttctt tcaatagttt tcttatacaa ggagcatttt ctccaacttc ataattacca
    15601 ctttcagtat aaatctttac ttcatactct tgcttttcta tgtacaaaac ttctttacta
    15661 ttaattgaaa aacgtttatc ctcgtattca acattaataa aattttcctc tttcatttcc
    15721 atccctccaa gtacttttat taatttaatt ttagtgtttt ttatcaatat aatctatcgg
    15781 aaatatatgt aagactgatt tacttaagat atttaaataa ttattattaa tattaatgta
    15841 taagtttatt ttttaacatt aatttttaaa gttaaaaaga ctttttttaa ttatatccgt
    15901 atatataagt atatctatct ttaataaaat aacttttata gaaattcatg taaaattata
    15961 cataaaaaag taattattaa cttttaattt aaatgaatcg tattaaaatt tatcttattt
    16021 tcctaatttt aatcttaagt attatatgta ttaatacaaa gattgaaatg ttagataata
    16081 ttaatagtaa aactattact aaaaaagcag ttcttctttc aggagagatt tttacaagat
    16141 taataatata atcataaagt acaaaaaatg acaataaatt tgctatcata ggaatacata
    16201 tgtatatctc ttttttactt attttaaaat ttttaaaaga agccttataa acataaagta
    16261 atatacctaa cattatcttt cccaagaata ttgcttcata tctatataaa ctgccactaa
    16321 gtaaaacatt catattatcc aaattgctta ctgttgctac taataacata cttaatccat
    16381 ttatacctat taatattaac caataaaaaa atgagattac cagacattta tatatattaa
    16441 ccttatagtt agacttatag aaaacgaaca ttacaaatat acatatcaag attctactat
    16501 tagctcctat atttatatat aaagaagaaa taatgagaaa aacagaaaag agaaaataca
    16561 tatttagttt ttttaagtcg gtctttgact gactagtata atcaaaaact tttttacatg
    16621 accatatttc tataatagaa gaaacaattg ttataaatgc atctaacata ataatatcct
    16681 caaaaatgat taaattatat tttttataaa atatatcata aaaaatacat actaattata
    16741 gcataaaaaa cacacttaaa tttaatataa ttatttaata tttttaacaa ctttctatag
    16801 tacattttaa aatatttaca gataagttac caataataaa gtttaaaaat ataaaaatca
    16861 tttcttactt ttagtttacc tataataaca aaagacaggt ttatattgta ataattaaaa
    16921 atagtaaaat atattttgat atggttaaat agaaataata ctttagatta atgaatattg
    16981 acaagttata aaattatttt aaattttaaa aatcatttct tatttaaata agtggtatca
    17041 tttaacttga tactccctat tccaattatt ttaggcattt tactaattag agaacctatt
    17101 taatattact ttatgaatag tgagtagctg attaaataat accatttatg gttttcagcc
    17161 atagattaat taaattctta gagttctatc ctgatataat tactcttctt ctaaattttt
    17221 aaaatgactt tcaaataaat ccaacatatc tttatcaaca acttcatcat catgtagtgc
    17281 tgaaattaaa ctctgtaaag aattgtcgtg tatattgctt aaaaaatctt ttgtctcaac
    17341 acttaaatac tccttatgtc caacaactat tgtataatga gtgtatctat ctattttttc
    17401 tgcacttaaa aaatgttttt taacaagtct tgataaaagt gtcaatgtag ttgtttgttt
    17461 ccaaccatat tttcgttcca tagcttcaat agtttctttt gatgttactg tatcattttt
    17521 ttcccaaata aacttcataa cttttaattc tgcttgtggt atttttgaaa ttgtcataac
    17581 cttacgcccc tctcaaactt ttattaaatt aatactatcg caatatagtt tttaagtcaa
    17641 agattttgtc gataataata ttttccaatt tttaccatag ggttattttt tatataattc
    17701 tatgataaat tacataaaaa atatttattt tacatcataa aattagaaat gtaaatagta
    17761 tgattattta gtcttatctt taataaacaa tccagtaaat ttatacatcc aaagtgcatt
    17821 cataaacaac aataaacttg ttgaaaaaaa tactgttcta atacctaaat gtgctgatat
    17881 ttgtccccct aaaaatgctc ctgatgaaca gcctatatac tgggcagatg cattatatcc
    17941 aaatattttt cccacatatt cttctggtgc aattttttta agcaagctgt taacagaagg
    18001 attaagccct gcaactgaca accctaataa aaatctcaac aacatcagtt gccatgcagt
    18061 ttttacaaaa gcttgtggta taaatataat cccagcccat aaaagtgaaa ttaacaatac
    18121 tttttgagga cctattttat ccgacatttt tccaagtttt tgagctgcta ataaatttgc
    18181 taatcctgat gcagaaaatg tcaatccagc tattaaagct acatttgatg caaaatttgt
    18241 aagttgtgat atatatatgg ttacaattgg ttctatagag tataaagcta gttgagtaat
    18301 gaatgtggtt acaaaaatag atgcaattaa aaatttattt ggcaatatat ttaattgttc
    18361 tttaggtata gaaattgatt cttttatttc tttaggttta aaattatcta ctacaaataa
    18421 aatagagatg ataaatgata caaacaacaa tcctcctgtt ataataaatg tacttttaag
    18481 acctaagatt gattctatat atcctcctac tgttggacca attaatgagc cagtagttga
    18541 tgcagttgca agagtaccca aagcccatcc tgaatgattt ttatctgtct gtgttgcaat
    18601 aagagtagta catgcagtag cataacccgt aaaaactcct tgaagtattc ttaatcctaa
    18661 taactgatag acattttgaa caaacgccat ggtaaatata actattccca tcccaatact
    18721 tgcacgtaaa agcattgatt ttcttccata tttatcagca gctttccctc aaattggcga
    18781 aaagaatgct gctactacaa aggtacatcc aaaaactata cctgaatact gttgtattaa
    18841 actaacatca gttaccccca actgtttaat atataatgaa agtataggtg ctatttggct
    18901 catccctata gaactaaaaa acatacctat ccaacataca aataaatttc ttttccacaa
    18961 ttccatatag aattacaccc cctgcatttt aaattttttc aataatacta agtttattaa
    19021 tttatgctat tgtttactca atatatccat cttttctttt ataatattgt ttaggaaatt
    19081 cataacttga aaataatagc ggtgataaag aataggagta acttccagca ttactaattg
    19141 aaatcaaatc tcctatgttt gcttttttca atttaacatt atttgcaatt acatctaatg
    19201 atgtacagag attcccaaca acatttacat actcttcttc ttttaaatta tttaatactg
    19261 aaagagaaaa ggcatgattt gatgtatata atggctcttg accttctaat tctactccac
    19321 aagtaatatt ttcaattaaa ttagaaataa taggtctcat aaatccatta agtccatttt
    19381 ttacaataag atattttttc cctcttgata ctttaatatc tacaatttta acataataat
    19441 tacctgattt acaaactatg aaacgtcctg attcaataag aagtcttgat tttagtgtgt
    19501 ttgaattagt aaaagcaatc ttttgagtta attttgaaag ctctttaaga ttaagtggat
    19561 tttctctaag attatcataa gctactccaa gtccactacc aaaatttata aattcaatat
    19621 taatatactt acttttattt aaatttaaag ctataaaaaa acatttttca taataatgtc
    19681 ctaacttttc aaaatccaat acctgagatt taatatgtat atgaatccca actacagata
    19741 cataatcaca catacttaac atcttaaaaa cactgctcat atcttctata tctataccaa
    19801 atttgcttga tttagcacta tcatcatcca tagtaaaatc aggatttatt cttattccta
    19861 ttttaacttt ctgattatac tttttagata tcttatttat aagttctatt tctgtaatac
    19921 tatctgcaat aattatgcat ttatctaaaa cagtttctat atcaatctct gttttgcctg
    19981 gtgatgaata ataaatatca tctttagaaa ctccacattc tgatgaaatc ataacttcat
    20041 tagttgaaga agcatctgct ccaaaacctt gctctgcaat gctttttaca attttgttat
    20101 atggatttgt ttttatagaa tataaaaaat caaaacctaa aatattattt ttcaattcct
    20161 tacatttttc aattatatta gtctcatcat atatataaca tggtgcattt tcttctataa
    20221 atttatagtc ttcatttgag tacatatttt ttacctccaa gattttaaat ataatcataa
    20281 attaagcaat tataatccta taagataatt ttacaataaa ttaccatagt taactacaca
    20341 catatatgta aaaacatacc taaaatattt ttaattatag atacattcca acttttattg
    20401 ataaaacaat ttgtttcaat tttattacaa atatacaaag tgtattgttg ttaacgtatt
    20461 ctcatagtat cctaaaacta tattaagcac taaaaactat tttaaaatac tgtggaggta
    20521 tcaatatgga agtatgcatt cttatgggaa gtcctaagaa aaaaggtaat actgctgcta
    20581 ttttaaaacc ttttattgag gaattagaaa attataattc aaatattgaa gtaatatggt
    20641 tatatgattg taaaatagag ccatgtattg cttgtaaaaa atgtcaggat aacttatttg
    20701 attttggatg ttattgtaaa gatgatgttc agaaaatttt caagaaaatt cttatgtgtg
    20761 atttaattgt ttttgctact ccaatttatt cttggtactg cacttctcca atgaaagcac
    20821 tacttgatag attggtgtat ggaatgaata aatactatgg taatgagaaa ggccccactc
    20881 tatgggcagg aaaaccagta gcacttatta caacatgtgg atatagacct gaaaagggtg
    20941 ctgatatttg ggaaaatggt atgaagcgct actgtaagca ttcacagtta aaatatatag
    21001 gaatgcttgt tgaacgtgat ttaggtaaac ctaattttat gaataaagaa aaagaagaac
    21061 attctcgtca atttgcaaaa caaatttatt atattttaga taattcaatt gaagtagata
    21121 actgctaaag acataatact ccaagtaaag tattgataat ataaataaat cttacataat
    21181 ttgcttattt aaaataagat atatattctt ctaaaaagta acaataatta aaatattttt
    21241 tatcatttca acaatgataa agaggcttat ctctattttg agacagcctc tttattgctt
    21301 tttatatgtt aattattttc atagtaaaca tccttaaaaa ctctttacat gttacttcct
    21361 tcaaaaccta ttactcattc ctactataaa ttttttataa attcagttat ttcttccttt
    21421 gttgcatatg aacttatcgc acctttaata gttgtactac gagctgcata ataattagaa
    21481 aattctaaat actcatttaa cgtttcttgt ttcatttctt ttatatcatt caatgttatt
    21541 tgtttataca ataaggtgta taaaaatgaa cctatataag aatctcctgc tccggttgta
    21601 tctattgcat ttactttaac ccctttacac atacctttta cttttttagt gtatgcatct
    21661 actccatctt ttccttttgt atataatact agttcaacat tccccacaaa gagtttatgt
    21721 aatgattttt ctatatcatt ttctcctgtt ataaattcta attcttcatc agatatctta
    21781 acaatatgag caaaaggtaa aaattctgat attgcctttt tacaagattg ctcactatcc
    21841 cataaaggta atcttatatt agggtcaaag cttattatac tattactttc aagtgcgtat
    21901 tctattgctt ttttatgtgc taatttcatt ggactatcta ttaaatctac tgaacaaaaa
    21961 tgtaggatat gacaattatt aaaccactct tttttaactt catcagcttc taaaagcata
    22021 tctgcacttg gatttctata aaatgagaaa tcacgattac catcttcttt taatgataca
    22081 aaagcaagtc ctgtatttgc tttacttgtt ctcaatacat aatcagtatt tacatttact
    22141 tcatttaata catctattat ataatcacca aatgcatctt ttcctaactt agatataaaa
    22201 ctcgattttc ctccaagctt tgctacaaca gcactaacat ttgctggagc tcctcctgct
    22261 actcttctaa attctgatac attttttaat ttaccaccat tttgattcgg tataaagtct
    22321 attaaagctt ctcctatgct aattactttt ttcatctttt ctacccctta taatgtgttt
    22381 ttataattca ataccttgct ttaatttaaa tttagtatac tcatactcta ctattttatc
    22441 actataaacc cttatttgat tagaattttc tttagcaaat attctactag taaatactac
    22501 ttctccttca tttataaaaa tttctaaaat acttctatcc gctagtactt tgatattagt
    22561 attttcatca atatttatat attcttttct atttgttcca tattcaacac cgaaaacttt
    22621 tttaaagttg cttctgtcta aagttacaat gtttcctaat ttgttaaatt ctaattttac
    22681 accttcatct tgtgatgaaa ataattccag cccaaattta ttgctgtcaa tattttttat
    22741 attaatatta agctcatata cattttcatc attttctata atattaatat tattctttat
    22801 agtattttgc ccacttgttt catcatatct taatttaatt aattcatcta ctggcatttg
    22861 caatagctta ttatttttta tatttaattt tcttggaaca gttagacaat gtgcccattt
    22921 gtttttatct gtagggtata aaacttctcc cattccagcc catgcaaata gtattatttg
    22981 tgagtccttt ataaatgttt gtggagcata gaaatcaaat cctttttcca gttctttcat
    23041 tgtatctatt tcaaatgtta aattatctaa atccatatta ccaatagcat atactacatt
    23101 atatatattt tgataatcaa atcctttagg ttctactcct tgtggtgaaa atattaatat
    23161 atctttttca tctatattaa tataatctgg acattcccac atgtaaccaa aatcttcatc
    23221 tatatttttt acatttaatt ctcctttaaa attccaatct atagagtttt tagattcata
    23281 tactattata gctccttttt tatcacttgt ttgtgctcca agtaacataa agtatttatc
    23341 ctttctttta aatacttttg ggtctcttac atgtcctgta tatccttttg gtatttcact
    23401 tactattgga ttattttcat atttagttat tttaccatcc ttgttcatta tagctaagtt
    23461 ttgatatgca tatctatctt ctgcactata ttttatattc cctgtataat acatatgcaa
    23521 ttttccatct acctctatcg cattacctga atatgctcca tgagattcat aactttcgat
    23581 tggtattaat gctacatctt ctctattcca gtttacaaaa tcatcagaac ttacataagc
    23641 ccaatgcttc attccatgag ttgcatcata tggataccat tgataaaata catgatactt
    23701 accattataa tatgctaatc catttgggtc attaagtagt ccatgttgtg gatgtatatg
    23761 aaatagtggt ttatatttat catttaaaga tatatttctt aatttattta attcttcttt
    23821 tgtcgattct aatatggttc tatatttagg ctctttgtac atatttttat tccttttctt
    23881 ttataatttt atatttgctt tattattgct atctaaaaca attcttagtc ttattacaca
    23941 atgtgctaca tcttttatat tttgtttatt cctatgtact ctaataatta ctttgatact
    24001 ttgttacaat cagttatttt tgcactcatt ttgctattct atcggtttaa ttaaaccggt
    24061 ttcgaaaccg gtttaattaa agattagcat aaatttattt cagatgcaat atttctttca
    24121 aaaaagtttt cctaattttt agaattatag tttttttagt aaaaaaatac acattactaa
    24181 tatatgtaat gtgtatctta atttttattt attgtacttt caccttctat taattcaaat
    24241 ttaatctcct ttaatagtgg ttcttttctt tcttgtatta aatttactat cgtatttgct
    24301 gctactttac ctgcattttt attttcaaac tttattgttg taagtttagg gcttataata
    24361 gacaatacat catatccccc aaatccagct acagatatat cttgtggtat atttttacct
    24421 ctcttattga tttctttcat tgttgctatt gctatattat cagttgcaca tattattata
    24481 tctggatttt cactttctag catttctcca cttctttgta tagacgtttc ttggtcaaag
    24541 tcagtataaa atacttttgc atcataccct ttatctttta acccattaag tactccattt
    24601 tttctattta aaccaactga tatatcactt tcatcaacac ctaaatatac aatgtttttg
    24661 taacctttat tagcaatata ttgacccatt ttatatcctg ctccataatc atcattcact
    24721 atgctattga taccatcaac tttttgtcca actacaacta tcggtatatc taacttttca
    24781 atttcattct tatgttcttt agttatttcg gtaccaatta gtattattcc atcaactttt
    24841 agactagcta attttgatat actatctatt tctgacctta tttttctatt agtattaata
    24901 attagtgatg tatattttaa ttctttgagt tcttcatcta ttgccatcat aattttagat
    24961 tttacaaaag aatctaaaca cggtgttata ataccaataa agtatgtttt tttagctctc
    25021 aaactttgag caaatgcatt cggctcatat ccatattcat ctattattgt ctttattttc
    25081 tctttagtag aatcactgat atctttatta tttagatatc ttgatacagt acttttagat
    25141 actcctgcca agttagcaat atcatttatt gttatttttt tcataggtta tcaccttcta
    25201 catttgatat attttattac attatattat atatttaaaa taaaatatag ttatttgtcc
    25261 agtcaatact ttttatatac tacttgtact atccattcca catccaaata attgagtctt
    25321 taacattaaa ttaattgtaa ttttcatttt ttgttgttat atttaatttc aattttatct
    25381 actatgaatt gataaatact ctaatttatt tataatataa aaataaggc
//
LOCUS       DAYAOY010000054        25119 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYAOY010000054 DAYAOY010000000
VERSION     DAYAOY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.054"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            57..470
                     /locus_tag="ACGI6B_002316"
     CDS             57..470
                     /locus_tag="ACGI6B_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751758.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            538..954
                     /locus_tag="ACGI6B_002317"
     CDS             538..954
                     /locus_tag="ACGI6B_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751759.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            955..1167
                     /locus_tag="ACGI6B_002318"
     CDS             955..1167
                     /locus_tag="ACGI6B_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751760.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            1564..1734
                     /locus_tag="ACGI6B_002319"
     CDS             1564..1734
                     /locus_tag="ACGI6B_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751761.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            2277..3155
                     /locus_tag="ACGI6B_002320"
     CDS             2277..3155
                     /locus_tag="ACGI6B_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFG9751762.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            3191..5086
                     /locus_tag="ACGI6B_002321"
     CDS             3191..5086
                     /locus_tag="ACGI6B_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9751763.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            5354..7261
                     /locus_tag="ACGI6B_002322"
     CDS             5354..7261
                     /locus_tag="ACGI6B_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751764.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            7251..7700
                     /locus_tag="ACGI6B_002323"
     CDS             7251..7700
                     /locus_tag="ACGI6B_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9751765.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            7800..8687
                     /locus_tag="ACGI6B_002324"
     CDS             7800..8687
                     /locus_tag="ACGI6B_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9751766.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            9092..10540
                     /locus_tag="ACGI6B_002325"
     CDS             9092..10540
                     /locus_tag="ACGI6B_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFG9751767.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            10566..12956
                     /locus_tag="ACGI6B_002326"
     CDS             10566..12956
                     /locus_tag="ACGI6B_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFG9751768.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            12958..13629
                     /gene="pgmB"
                     /locus_tag="ACGI6B_002327"
     CDS             12958..13629
                     /gene="pgmB"
                     /locus_tag="ACGI6B_002327"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFG9751769.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            13668..15038
                     /locus_tag="ACGI6B_002328"
     CDS             13668..15038
                     /locus_tag="ACGI6B_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9751770.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            15385..16227
                     /gene="licT"
                     /locus_tag="ACGI6B_002329"
     CDS             15385..16227
                     /gene="licT"
                     /locus_tag="ACGI6B_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9751771.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            16372..18300
                     /locus_tag="ACGI6B_002330"
     CDS             16372..18300
                     /locus_tag="ACGI6B_002330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9751772.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            18361..19788
                     /locus_tag="ACGI6B_002331"
     CDS             18361..19788
                     /locus_tag="ACGI6B_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFG9751773.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            20199..20975
                     /locus_tag="ACGI6B_002332"
     CDS             20199..20975
                     /locus_tag="ACGI6B_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9751774.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            21015..21356
                     /locus_tag="ACGI6B_002333"
     CDS             21015..21356
                     /locus_tag="ACGI6B_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFG9751775.1"
                     /translation="MQEITSKFKECRKAISAMGDETRQLILIALLESDFNGIRVGEIT
                     KKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELINLIFVGIQ
                     HISENDIRKGD"
     gene            complement(21532..22281)
                     /locus_tag="ACGI6B_002334"
     CDS             complement(21532..22281)
                     /locus_tag="ACGI6B_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9751776.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            22503..22631
                     /locus_tag="ACGI6B_002335"
     CDS             22503..22631
                     /locus_tag="ACGI6B_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751777.1"
                     /translation="MKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFVFLFTIT"
     gene            22759..22941
                     /locus_tag="ACGI6B_002336"
                     /pseudo
     CDS             22759..22941
                     /locus_tag="ACGI6B_002336"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            22969..23184
                     /locus_tag="ACGI6B_002337"
     CDS             22969..23184
                     /locus_tag="ACGI6B_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFG9751778.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            23216..24403
                     /locus_tag="ACGI6B_002338"
     CDS             23216..24403
                     /locus_tag="ACGI6B_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HFG9751779.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            24595..24999
                     /locus_tag="ACGI6B_002339"
     CDS             24595..24999
                     /locus_tag="ACGI6B_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFG9751780.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9994 a         2588 c         4372 g         8165 t
ORIGIN      
        1 tatatattta taacataagg tgttataaat atataacttt taaggggtga ttttatatgt
       61 ctaaatcaga gcttggtgtt aaaacaaatc ttgtttcaga gttacttatg atatcctatt
      121 tttttataga ctcattatta aaaaagttta tttttaaaga gggaaatctt tgtattactt
      181 ttataatttt attcgctttt tgggcatgtg acttcatgta taataaaaaa tataatgatt
      241 tagttgacga ggcgtcaaaa cttatgttat taaaggttga tcatattgca tcaaaaataa
      301 tgttatattc tattttgcta atagctgttt tcttttcagc aaattataca agaggtttgg
      361 atatatcaaa tttggatata ggtgtaattc tcttagctat tttatttata caatctttat
      421 ctaaattaat tttgtatata cattttgata gaaagggaat atgcaactaa gtgataatat
      481 tgataaaaac gaaaattaaa gagtatagag aatataattt ttaaggagtg attttatatg
      541 tctaaatcag aaactggtgt aagatcaaag cttatcctgg agttgcttgt aataacttat
      601 ttttttatag ctgtattagc cactaagtat atttttaaag gtccaagcac tgcttctctt
      661 ccagggttga gttggttaat agtttttgga tgttattcaa tttataataa aaaatataat
      721 gatttagttg atgaatcatc tgaatttata ctatcaaagg ttaataagat tgcaataaaa
      781 ataatgatat attctatagt aataatatct attttctttg caacacctta tacaagtaat
      841 ataaatatat caaacctaga tacaggtata atcctcattg cagttttatt tatacagtct
      901 ttatctaaat tagtgttgta tatatacttt gataaaaggg ggatatgtaa ctaagtggca
      961 atattgatga aaacaaaaat taaagaatac agagaaaaac tccttatgac gcaaaatgaa
     1021 cttgctaaat tggttggggt aagacgtgag actattgtac acttagaaaa tgggaaatat
     1081 aatccttctt tgaagttagc tatggatatc gctaaagtat tcgatactac agtagaaaat
     1141 ttgtttgaat ttattgaaga agaatagtgg aaagtttata aacatttata agatggataa
     1201 taaaatatag ttatttaatt attaagtttg ttaaaaatat ttatataata taatatatga
     1261 ttaaggtcag attataattg ttttaagata aaatgtaata aaatttaatg gagaaatttt
     1321 ataggtagat aaattttaat atttataaga ttatgttaag gaaacactta aaaattagat
     1381 taaaattgtg ggtatttaaa tcaactaaaa gatattgact atttagtcaa tatctttttt
     1441 tattaaaaag gaatatacat ataaataggt tagattaaaa attgggataa atatgattta
     1501 tatagcatca tttaattaaa aggagctata ttagtattca ttcatacaat tggataatat
     1561 acaatgtcag ccttgcttta tggtgtttgt aataaatcta tttcagtttc tattgtaatt
     1621 aatatgcaag aatttaagat aggagtatct atggcttatg gtgtattagt aattggtata
     1681 tgctatatag cattaggaat aatttttaaa ttagatagaa aaagatttac ataagataac
     1741 aaaggctaat ttataaattg gtgcttagaa aaccaaatca ctttattagc tagagatgaa
     1801 tttggagggt gtcactttgg tgttttatgt aaagtttatg gaagtaaaac attagataat
     1861 cgtgagtaag atttctataa atcgtatata ctattggaat ccaagaaaag gtattggcca
     1921 aatagctaat atctttttaa tttttttgac tctaatatag cattaactat tgcatgagta
     1981 tctttaaatt tatagttaat gtatcttaat tgtttattat aaaatctagg aaggctaaag
     2041 cataaaacac ttaaaaagag aagatgtaca tgagaaatat aattaactat gatgcagact
     2101 tctaaaaatt atttagtgaa aaggaactta gagtgtataa taaaagttcc ttttcactaa
     2161 ggaaaacgat tgttattttc tgacattcat taccctgata ttatttaaat ataaacaaca
     2221 acaaaattaa caagaagcaa attcaaccta aaatttaaaa tatcgggagg tacaatatgt
     2281 taataaacat gaaggaaatg ctaaaagtgg cacaagaaaa tcaatttgct gtaccagcat
     2341 ttaatatagg aagtgggcaa atacttaaag cagtagtaca gagtgctaac gaaaaaaatg
     2401 caccagtaat acttgcaata cacccaaatg agctaagttt tctaggagat agctttgtag
     2461 ctagttgcat agaagaagca aataagagta aagtacctat ggtaatacac ttagatcatg
     2521 gggaaaataa agaacaaata ttaagggcaa taagatgtgg atttacatca gtaatgattg
     2581 atggctcaca tttaccatat gaagagaatg tagctatatc tagggaaata gtagaaatag
     2641 caaaaggatt aaatgtgtcc gtagaaggag aacttggaac aataggaaca acaggaacat
     2701 caagtgaagg tggtactgat gaaataatat atacagattc aaaattagct aaggattttg
     2761 ttgaaaaaac aggagtagac acactagcta ttgcaattgg tacagcacat ggtatatatc
     2821 caaaaggttt taaaccagag cttaaactag atttattaaa agaaataaga gaagtagtag
     2881 atataccttt agttcttcat ggtggttcat caaatccaga tgaagaaata gcacaagcag
     2941 ttaaactagg agtttgtaaa ataaatattt cgagtgatgt taagtcggca tattataaga
     3001 agtgcagaga actattagaa caaaacccaa gcttatatga gccagatact atatatccac
     3061 catgtataaa agctgcaaga gaagttatag agtttaaaat gaatctattc aatgctatag
     3121 ataagttaaa gtgcttttat aaaaataaaa attaattatg aggtaacaaa ttaaaaggag
     3181 gggagaattt atgataggta aaatactacg aagcgattgt attgtactag ataaacaatt
     3241 acaaacaaaa aaagatgtga ttaattatat ggcaaatcat ttgtaccatg gaaattatat
     3301 aaataataaa gaaaagttta tagctgatat taataaacgt gaaaaagaat catcaactaa
     3361 tatgggtaat ttaatagcta tacctcatgc taagtcaaat gtagtaaatc aacctgtaat
     3421 gatgtattta agaacagata gagagattac agataatgat ggtcaaaaac ttaggtcatt
     3481 ttttatgata gcagttccag aatcaggttc agatgaacat cttaagatta tatctaaact
     3541 agctacatat ctaatggaag aaaattttct taagcatcta ctaagtgcag aaactccata
     3601 cgaaatatta agtatattta ataataaagc tgcagaatca ggagatttta gtaataatca
     3661 aaataaaggt cttatagttg ggataacagg atgtccaaca ggtatagctc atacttttat
     3721 ggcaaaaaaa gcattagagg aagcagggca agaacttgga tatgaagtta aaatagaaac
     3781 tcatggagct ataggtgtag aaaataagtt gactaaagaa gatataaaaa gagctgaata
     3841 cgtaataata gcttgtgata aagaagttca actgagtaga tttataggaa agaaagtgct
     3901 acaaaagaaa gtagtagatg ctaagaatgt ttcattagct aaaaatttaa ttataaacgc
     3961 tgaaaatgga atggctaaag aaattaaacc aggtggaaag actttagaag attcaaaaga
     4021 aatgcttcaa gggtcagaga aaaagggtgt ttataagcac ttaatgagtg gtgtatctga
     4081 aatgattcca tttgtagtga taggtggaat tggtatagcc atagctttta tgtttggtat
     4141 atttgcatca gacccaactc atgaaacata caaccctata gctggattct ttggacaact
     4201 tggaggcgat tcagcattta agttatttat accgatactt gctggtttta tagctaaatc
     4261 aatagcagga agacaaggat ttgctcctgc tatgatagct ggttttatgg cagttattgg
     4321 aggttcaggt tttataggtg gtatggtagc tggttttgca acaggatata tagctttagg
     4381 agtaatgaag ataacagaaa atttaccaga tgtattaaaa ggagtaaatg ctgtattgat
     4441 atgtccactt ttgattgcat ttacgagtgg agctataatg ttctttgtag ttaatacgcc
     4501 agcatcatgg ttaaatgaag ctttacaatc ttggttacaa agtatgtctg gaactaataa
     4561 agtattatta ggtgcaatat tagcagggat gatggcatca gatatgggag gacctattaa
     4621 taaaacagct tcagcttttg gattggctat gttttcaagt aatatatttg aaccatcagc
     4681 agcattaatg gtaggaggaa tggtacctcc attagcaata tcattagcta tgttaatatt
     4741 caaaaataag tttactaaag aagaaagaaa gtcatctcta ccaactgcta taatgggggc
     4801 atctttcata acagaaggcg caataccgtt tgcagctgca gacccattaa gaatgattcc
     4861 agcaaatata attggttctt gtataggtgg ggcaatatgt atgagtttag atataacttt
     4921 aatggcacca catgggggaa tatttgtaat accatttgca tgtagtgcac cagttgcata
     4981 tatagcttgt atattgattg gtacagtaat aacagcattg attatagggg taacaaagcc
     5041 tactataagg agtgaaaata gtgaagttga attacttggt gcttaaatta taaataatag
     5101 taaaaataat taatggaata acaacaagta tatagaataa taataaggat atttagaatc
     5161 agttaactat ttctaaatat ccttaatttt tattttgaaa ttatattatc tattatatca
     5221 atttagtaag gtgtatcata aattctttta tgatacagaa gtatcttagt aattttacat
     5281 agttagtaaa tatgttaaaa tagtacttaa tataaacata gaaatatata aacataggta
     5341 gggagggcta agtatgcttg tgtcgaggca aattgaattt cttaaattga tactaaacga
     5401 aaataaatat agacctataa aatactttaa agataaatta aatgtatctg ataaaacttt
     5461 acaaaaagat ttgaagatga ttgagaaata tttagaaact tttaatataa aaattgatat
     5521 aaaaagaggc tatggaatat taatagaaga tttagcaaag aagaacatag acttaattaa
     5581 tagtttaaat atacaatcga aggaaaataa atctttatca gtagagcaga gaagaatgga
     5641 gattttaaag tatttattgc taaattcaaa taatataact tcaataaatc agttagcaga
     5701 taaatattat gtaagtaaaa cttctatagt aaatgatttt aaatatatag aggcatggat
     5761 aaaagaatac aatttaaaac ttaataaaac tttagaagga actaagatta taggtaaaga
     5821 agtggatata aggaaatcga ttgccaaaat gatggatgat ttattagatg aaaattatga
     5881 acaacatgat atatctgagt taacaagatt agattctgct acatttagtg cattgataaa
     5941 tttatttgat attgattcta ttatatttgt agaaactata attacagaat tagaatacaa
     6001 tttaggatat actataaatc agccctacta tataaattta attacccata tacttatatg
     6061 tttaaagaga atcgaagaag gtaatcaaat agaatctcag gaagaaagag aaatttctgt
     6121 agataatttg gacgagttag tatataaaaa tgtcactaca cttatagata agatagaaaa
     6181 tagatacaga gtaaaaataa cgaaagatga aattatgtac atatatattt ttttggtatc
     6241 atctggattt agtagcgaaa ataatagtag ttacaatgat gaatcagata gattaagtga
     6301 aagtgaaaaa gtttcatcta taatgataga gaatatgtca cgatttttaa atataaatct
     6361 caaaaatgat gacttactac agaaaagtct agcatctcat gtaaggccta tgttaaacag
     6421 attaagatat gatatccaaa taaaaaaccc gttgttagga gagatacaag agcgattttc
     6481 agatgtgctg ggattatgta tgatgactat aaatattatg acagagtatg ataatctgaa
     6541 gaatattagt atagatgaag tttcgtatct tgccacatat tttcaagcgg caatagaaag
     6601 aaatatgagt agtaaaagag taatagtagt ttgtcataca ggatatggaa catctcagct
     6661 ccttgcagcc agattaaaaa gagaatttcc tgaatggact atagttgata tagttcctat
     6721 gcatcagttg agtaagcgaa atttagatga tgtagacttt gtaatatcta ctgtaaactt
     6781 agatttgaaa gataaattac atatagttgt atcagtacta ttaatggaaa gtgatattaa
     6841 taatataaaa aatgctttac tttctaaacc taaaaaaagt atgaacttag atacaaactt
     6901 attaggaatg tatttagaag ataatatata ttttaatttt gatgtagctc agatggaaag
     6961 tttaataggt gtgaatttag ggagtaaata tgaatctatt tcattaggaa aagatttaaa
     7021 tatatatata cataagcaaa gtaaattaaa taaagggata atgaatatca ataatgttac
     7081 tagaacgatt gatatttatt tagaaattag taatcaaagt tttttaaaga atattttaat
     7141 ggaaatatca aatttatata ggcaaaagaa ctatataaac tatataatag attgcaagag
     7201 caaagaggat attaaaatat tatttaagaa taatctattg gagaaaacaa atggaaatta
     7261 gagaaatatt aacagaagaa agcattattt tagatttgaa atcaactaca aaagaaggca
     7321 taatacagga attagtagat aaattatacg atacaggaag tataacttta aaaagtaagt
     7381 ttataaaggc attaaaacat agagaaaaaa taggctctac tagtataggt tatcaaatgg
     7441 ccatgccaca tggaaaaaca aaatatgtaa agaaggcatc catagtaata ggtaaatcta
     7501 aaaaaggtgt agattggcca agtatagatg gcttacctgt aaaggtaata attatgtttg
     7561 ctatgccaca atctggaggt aatgaagata tgatattaat atctaaaata gcttacattt
     7621 taggtggtaa aaatataggt gaaagacttt taaatgcaca tacaaaaaaa gaaatattag
     7681 atatatttta tgaagtgtag gtattgtata aattataaat ttgaggtttt taaaataaaa
     7741 tgtgtattct tttaaatagt atagattaat ttttagacac tagagaggag aggatatata
     7801 tggcaatata tttagaaatg cctaaattaa tacatgaatt tccttttcgt actctatcaa
     7861 atgaaggaga agtattaaca acaccacact ggcataagga aatagaaata ttacttataa
     7921 cagaaggtgt agtaaatcta tttataaatg acaagcctat gcaattaaaa aaaggcgaga
     7981 tagtgataat aaaaggagga gatattcatt atatactgcc atcaccggga agtgaaagat
     8041 tagtatttca atttgatata tcttttttta atgatttaat tttattaaat gaggagaaag
     8101 aaagcttaat taatttattc agtagtataa aaaaatgtag tagagattgg tcagcaaaaa
     8161 taagaaagtc agttttcgat atattgatag atatctacaa tgaagaccta tacaaaatag
     8221 aaggatatag ttatgcaata aaaggtaaaa tgtataattt aattcctata ttatatagac
     8281 agattcctag agaaattgaa gaaaatacag tagaatatga aataaactct aaagagatat
     8341 tagagagatt gaacaatata ttcaaatatg tagaaaaaaa ttataaacaa cctataaaat
     8401 tagaagatgt gtcttatgaa gtaggattta gtgtatatta ttttactaaa tttttcaaaa
     8461 aaaatacagg aaaaacattt ataacttttt taaatgaata tagattagaa aaagcaaagt
     8521 ggatattact taataacaat atatccattg tagatttggt tgaagaagtt ggttttggaa
     8581 gtacaaaaac tttttacaga gtatttaaag aaaaaattgg agtctctcca ttagaattta
     8641 aaaaaagata taatagcttg gagaaaaaag ggagtgtctc aaaatgaact tagttcatga
     8701 ggcactttct tttatatgaa atcaaatata ttttcagtat ttcaacaatg aaaattccat
     8761 tcatttataa agtctaaatt ttagttctca atacaacctc tatttcgaaa taaatccttt
     8821 tatatatata aatatacagc aatatatgag aataagataa caataatgag gatataaaaa
     8881 tgtatttgct atatatataa ttatatacat agatatctaa tattgatatt taatatgaaa
     8941 gtattgaaaa tagacaatat taattatagt gagtaaaaaa tataattgga tttttaaatt
     9001 gaattattta aaaggtatag tatgaaaaaa atcttataac taaatatgag atagaaatta
     9061 taaaatttta tactgtgaaa gaggtagatg catgaaatta gggaaagatt ttttatgggg
     9121 aggggcaatt tcagccaatc aaagtgaagg attttattta gatggaggaa aaggtttatc
     9181 tcagtttgat aaactcccat taaatgataa gagattaaaa aatgtatgtt tagatgagga
     9241 taatatttta aatgaaaata atcaatacta tccaagtcat atagggatta aattttgtga
     9301 aaattacaaa gaagatatag caatgttagc agaaataggt tttaaatgtt ttagattttc
     9361 tatatcttgg tcaagaatat tcccaacagg agaagaaagt gaaccaaatg aaaagggatt
     9421 acaactttat gataatataa taaaagaatt aaaaaaatat aatatagagc cattaataac
     9481 gataagccac tttgatatac cttattctct agttgaaaaa tattctgggt gggctgatag
     9541 aagagtggtt aatttatatg taaaatatgc tgaaattttg atgaaaagat ataaagatga
     9601 tgtcaaatac tggattcctt ttaacgaaat gaatatgata atgcatattc catatatagg
     9661 tgctgggtta acttttatag ctgatgaaaa tcaacttcag aaaaaatatc aagcagcaca
     9721 ccatcaatta ttagctaatg caaaaactat agaaatagga aggtatataa ataaagaatt
     9781 taagtttggg tgtatgttag ctgctggaaa aacatatcca tacacatgta aaccagaaga
     9841 tgtatttgca actttagagc atgagaggca taatttgttt ttttctgatg ttcaagttag
     9901 gggtgtgtat ccaagcttta tgagttatta tttaaaatca aatcaaataa aattaataat
     9961 aaaagaggaa gatttatcaa cacttaagga taatactgta gattttgtat cttttagcta
    10021 ttattctagt gcttgtagtt cagctagagc tgaaggttta gaaaaatcaa aggcaaacgg
    10081 accagaaacc ataaaaaatc catatcttcc aaaatcaaat agtgtatggc aaaacgaccc
    10141 tgtagggtta agaataacta tgaatcagtt atatgaaagg tatcagttac ctttatttat
    10201 agtagaaaat ggactgggta ctcaagatat tgtaaatgaa gatggaaaaa taattgatga
    10261 ttatcgtata gattacatga aagaacatat taaaaatatg attcttggag taaatgaaga
    10321 tggaatagag cttataggat atcttatgtg gggatgtata gatgtaacca gtgtaagtga
    10381 aggaaagatg acaaaacgat atggcataat tcatgttgat tctgatgact atggaagagg
    10441 ctcatttaaa agaactaaaa aacaaagtgc tttttggtat aaaaaagtaa tagaaacaga
    10501 tggagaaatt ttatttaatg atatggaaac tcatgcataa ttcattttaa gaaaggagtg
    10561 ttattatgag tttaatattt actactaagg accaacttgt agagctaggg tttagtaagg
    10621 atacaataaa atccaatgaa acgttgttta cactagctaa tggacattta ggagttagag
    10681 ggaatattga agagtctgat tttaataatg agtatgtaga taatatgggt acatatgtaa
    10741 atggatttta tgaagaatct ccaattacat atggagaaag cgcttatggc tatgcaaaac
    10801 aaaatcagac tatctgtaag ttacctaatg ctcatatagt aaatttctct attgaaggag
    10861 attgctttga tttatcaaaa ggtattacaa gtgaacatac taggatacta gatttaaatg
    10921 aagggattct taaacgttca tttatatggg aaaactctcg tggtagaaaa gttaaagtat
    10981 ctatagaaag attagtatca caagatatag aagaattaat gatgatttca tatacaataa
    11041 caccattgaa ttttaatgga aaaataaaat ttaaaaataa aatggaaaat agtatggaga
    11101 agtttaattg tgaaaaagta gatgacccaa gagtggcatc tataaataag aagcaattta
    11161 aaattaatgt aaaaaaatat aacaataagt attttatgaa tttagttaca gaaagaagta
    11221 gattagggtt atggtgtggg tttgattcat atattacttg tggaaatatt acaaatgaga
    11281 cctataatgt ttcagatgaa tgtattgagc gagaaataga agtctatgcc aaaaaagatg
    11341 aaaggttaaa tttagaaatt gttgtaggct atagtagagt atattctgat ttaaatctaa
    11401 atgaagaacg tttaaaatca ttagaatgtc tggagtcgtt aaaatgttta gaatcagaag
    11461 cacatttaaa atcattagaa ttggttttaa gagaagcatg taatattgga tttgataaag
    11521 tcaaaaatat tcagagaaag aaaatgaatg agttttggac aacctctgat gtagagattg
    11581 aaggcgataa aactcttcaa ctggggataa aaaccaattt atttcatatt tatcaatcag
    11641 caggaagaga tggaaagact aatatatcag caaaaggtct tacaggtgat ggctatgagg
    11701 ggcattattt ctgggataca gaaatgtata tattaccatt ttttatatat actcaaccat
    11761 ccattgctag agctttatta gattatagat actcaatctt agatgcatca agagaaagag
    11821 ctagagaact tggaataaat aaaggttgct tatattcatg gagaactata aatggtaatg
    11881 aatgcagtgc atattatcca gcaggaacag cacagtttca tataaatgca gatatagctt
    11941 atggtgttag aacgtatttt gaagcaacta atgatgaaga atttatgatg caaaatggct
    12001 tagaaattct tatagaaaca tcaagatttt gggttgaatt tggagattat atagaatcaa
    12061 aaaataataa attctgtata aatggagtaa caggaccaga tgaatataca gcaatagtaa
    12121 acaataatta ctacactaat ttaatggcta agtataattt aatatatgca gtacagatgg
    12181 tagataaatt taagcataat ttattttcaa atgaagtatt tagaaaagtt aaattacaag
    12241 aatatgaagt gaaaaactgg ataaatgccg ctcaaaatat gtatttacca tatgatgaag
    12301 agaagaaaat aagcaaacaa gacgattcat ttttagaaaa atcagtatgg gattttaaaa
    12361 atacaccaaa agaaaaatat ccattacttc tccattatca tccattaaca atatatagat
    12421 atcaagtaaa taaacaagca gatactgtac tttcagcgtt gcttttccct gatgagttta
    12481 gcttagaaca aaagaagaga gattttgaat actatgaaaa aataactact catgattcat
    12541 ctttgtcaag gtcaatattt ggaatgatgg caagtgaaat tggaaatcat gaaaaagctt
    12601 ataactattt tatggacaca gcattaatgg atattaataa cctacaaaaa aatacaagtg
    12661 atggaataca tgctgctaat atgggtggaa catggatgag tttagtatat ggatttgcag
    12721 gaatgaaaat aaaaaatgat aaattaagtt ttgaaccaag gatacctaaa cactggaaaa
    12781 atattaaatt taatatatta tttagagata atttagtttt tgtaaatata gaagaatata
    12841 aatcttatta taagttaatc aaaggtagaa atataaccat attacatgat aataaagaaa
    12901 taaaattatc aaagaacata atagaagtta ataattgttt taaagggagt aattaatatg
    12961 aaagcagtct tatttgatct tgatggagtt ataacagata ctgctaagta tcattttgaa
    13021 gcttggcatg agatagcaaa atcgctagat atagaaatag atgaaacttt taatgaaaaa
    13081 ttaaaaggca taaacaggga agaatcatta gataaaatat tgttatctgc taataaagta
    13141 gatagttata ctaaagagga aaagttaaag gtgtgtgaag aaaaaaataa catttatata
    13201 aagttaataa actcattaac atataaagat atattaccag ggataaatga gcttataaaa
    13261 gaacttaaaa aaaataatgt gaaattggga atagcatctg ctagtaagaa tgcacctagg
    13321 atattagaat ctttgggagt aaaagatgaa tttgattgta tagtaaatcc atctttatta
    13381 aaaaggggaa aaccatatcc tgatattttt attgaaggga gcaagatgct aaatttaaaa
    13441 caatatgatt gtgtaggaat agaagattct tattcgggta ttaaagctat aaatgctgcc
    13501 aatatgacat caattggtgt ggggaaaaaa gaagtactag gaaaatgtga ccatattgta
    13561 gaagatacta gcttattaaa ttacaataca ctattacaag cttggagtaa aaagattaat
    13621 ttggattaaa attatattaa aaattatttt aaaagagagg ataaggtatg aagaataaat
    13681 atgaagatgt agctcaattg ataattgatg gacttggagg aaaagataat ataattgact
    13741 taactcattg tatgacacga ttaaggttca tcttaaaaga tgaaaagaaa gtcaatgcag
    13801 ataaattaaa atctatagat aaggtagttg gagtaaactc aacttcaact caatatcagg
    13861 tcataatagg taatgaggta ggtgttgtat ttaatgcaat aataaataag gggataaata
    13921 ccacaggaga tattaaaagc gatataaaaa ataaaaataa aaaaaatagt attttaaata
    13981 atataataga tacaataact ggttgtatga cacctatgat accagccctt acagcatctg
    14041 ggatgataaa agttatatta tctctttgca ccacttttaa ttggctatca tcaaattcaa
    14101 gtacatatag agtattagat ataataggag atgtagcatt ttactttatg cctataatac
    14161 ttgcaataaa tgcagctaaa aaatttaatg ttaatacatc aatagcagtt atagtagtag
    14221 gtgtgttttt acatccaaat ttctctgcat gggtatcaag tggggaccct atatcattta
    14281 taggagtacc aattcaaggt gttatatatg cagcatctgt tatccctgca cttttaacag
    14341 tatggatgat gtcatatata gaaaatttca ttgataaatt aacacctagc atgttaaaaa
    14401 caattttaaa tccaacttta gtattattaa taagtgcgcc aatagcatta atagtaattg
    14461 gaccaatagg aaatttctta ggagagggat tagctagtat aataaattta ctacaaggaa
    14521 ggttaggatt tataatggtt tgtttattag cagctgcaat gccatttatc gttatgacag
    14581 ggatgcatca tgcattgact cctatattca tatcagcatt tgcaactaca ggacaagaat
    14641 cgctaatatt aatatctcaa gtttgtgcta atttagcgca aggtggcgca acattagctg
    14701 tagcattaag aagcaaagat agtaagatga aacagttagc atcagctgca tggatatcag
    14761 ctattatggg tataactgaa ccagctcttt atggagttac attgaaactt aaaaaacctg
    14821 ctatgttagc atctatagga gcaggaatcg cagggttatt tgcaggaatt actcatgtga
    14881 ctttatatgt tccacaaaat agtataatgg cagtacttgg attatcaggg gaaaagggga
    14941 tttcaaatat agtaaatgga cttatagtta tatttatagc attggttgct ccatttatta
    15001 tgacatatgt ttttggagtt gaaaaaaatg ttaaataaag tacagataat gtagtagagg
    15061 aagtataata ttaactattt acattaatag aatttttatt attattgact ataacttgtt
    15121 ttgtatttat attgatgcaa agaaataagt taatattaat aagaataatt attataactg
    15181 acaaaaagca acataaaaga ttgatgaagt atatggaaat aaaaagttga caaaatatgt
    15241 ctgatgtttt atcatattaa ttggaattag gtagtaattg tttaagtaca agctaagctt
    15301 attcattaat aatagcagta tcctaggaag gagtatgccc attattatgg ataagctttt
    15361 tttatgctta aaaggaggat aatcatgata attaataaga tattaaacaa taatgttgta
    15421 atcacattag atgataatga tgaagaagtt atcgttatgg gtaaaggaat tggttatcaa
    15481 aaatcaaaag gcaatctaat agataaaact aaagtaaata aagtatttag aatatcaaat
    15541 aaagaaatat caaacaaact acaagaatta ctcaataata ttcctataga acacatgaaa
    15601 ttatcaagtg aaattataga atacgcacaa ataaagttaa acaagaagct aaatgaaagt
    15661 atttacatat ccttaagtga ccatacatat tctgcaattc aaagaatgaa agaaggaata
    15721 aatgtaaaaa atgcaatcct ttgggaaatt aaaagatttt ataaagaaga gtttgaaatt
    15781 ggaatgaaag cactagatat tattgaaaac aaaacaggta taaaattacc agaagatgag
    15841 gctggattta tagcctttca tatagtgaat gctcagttaa gtgaaggtca tactttggca
    15901 tctgatatta cgaagttaat acaagaagta cttagcatag ttaggtatca ttttaggata
    15961 gaatttcatg aagaatcagt gttttattat agatttataa tgcatttaaa gttttttgcg
    16021 caaagattgc ttttagatag tgcacatgaa ggtgagacag acaaagaatt actaagcatt
    16081 attaaatcta aatataataa agagtttgaa tgtgtagtta aaattaaaaa tttcattaaa
    16141 aaacaatata actacatatt aacagatgat gagcttatat atttaacaat acatttagct
    16201 aaagtagtta aagactcaaa tatgtaagtg aaataatttt tcagcagtat attaagaatg
    16261 attgaaagga gtgatactaa caaaattata cttatggttg gatggtaaca ttgagttggc
    16321 caaaccttca aatttcaaaa aattaattta aacgaaatat ggaggagaaa aatgggaaaa
    16381 tacaaacaat tagcacaaga gatagtaaac aatgttggtg gaaaagaaaa tattagtgga
    16441 ttagtacact gtattacaag attacgtttt actttaaaag atgaaagtat agcaaatgac
    16501 aatgtattaa aaaacatgga aggtattgtt actgtaatga aaagtggagg acaatatcaa
    16561 gtagttatag gaaatcatgt tgaagctgtc tataaagatg tagttgaaat aattggttta
    16621 gatgattcaa gtacaagttc agaaactaaa aaatcaggta atatactaga taaaggtata
    16681 gatataatat caggaatttt tcagccagtg ttaggaataa tggcagcttg tggtatgttg
    16741 aaaggattta atgcattatt tgtagcaatg ggattatata gtgatgtatc tggtggttat
    16801 atggtaataa atgcagcggg agatgcatta tttacattcc ttcctttgtt cttaggatat
    16861 acttcagcta agaagtttgg attaaaacca atgttgggat tagcactagg agctgcagta
    16921 tgttatcctg ctatacaagg tagcagtata tcagcaggag cagatgtgat gtacacttta
    16981 tttaaaggaa caatgtttgc atcaccagta tatattgatt tctttggact accaattgta
    17041 tcaattgatt atactggaac agtcgtacca attatattta tagtgtattt tgcttctagg
    17101 tgtgaaaaat tatttaataa gtgtgttcca aatttagtta agtttttctt tgtgccaatg
    17161 cttacattat taataacagt gcctgttgca cttatagtta taggccctat agcaacattt
    17221 ggttctacac ttatttcaca aattgtaatt tcaataagag attttagtcc actattggca
    17281 ggtgctatag taggatttac ttggcaaatt cttgttattt ttggtatgca ctggggattt
    17341 attcctgtat atataaacaa tgttatgact ttaggatatg ataatgttat gatgccattc
    17401 tttgcctgta catttgcaag ttcggcagtt gtgttggcaa tatttttcaa gactaaagat
    17461 aaaaaactaa aagagatggc aataccaaac tttatatctg gtatatttgg agttacagag
    17521 ccagcaattt atggaatatt attgccttta aagaaaccat ttataataag ttgtattgct
    17581 agtggtattg gaggcgcttt ctatggattc tttaatctta gaaaatttat aactggagga
    17641 atgggaatat ttgaacttcc agcaatgatt gaaccagatg ggggaatggg taacttaata
    17701 gtagctcttt ctggtatagc tatatcaatg gtcgtagctt ttgtattaac tatgatttta
    17761 tataaagatg agaaagtaga agagtctaaa aaagtaagaa ataagggtaa agaagaaata
    17821 aaagaagtca agtctactaa attagaaaga gaaatagtaa caagtcctat aaaaggtgaa
    17881 gtattaaaat taagtgatat agaggatgca gcatttgcat ctggtgtact aggtcaaggg
    17941 gtggcaataa tacctagtga tggaaaagta gttgctccag tagatggggt agttacaacc
    18001 ttatttccta gtctacatgc tatagggatt ctttctgata caggtgttga agtgcttatt
    18061 catattggat taaatactat tcaattagag ggaagaggtt ttgaggcatg tataaaacaa
    18121 ggtgatagaa ttacaaaagg tcaaactata ttaaactttg atgttgatgc aataaaagag
    18181 ttggggtata gtactgtaac tccaattgtt attacaaatt catcacaatt tttagatgta
    18241 gtagaaacag aaagtaaaaa tattgaatta gaagacaatc taattacagt cttattttag
    18301 taaagattga ggtattagca taatctagtg ctttggttat aattttagga ggctttgact
    18361 atgagtttta agaatgattt tttatgggga ggagctactg cggccaatca atgtgaaggt
    18421 ggatacaatg aagacgaaag aggattggca aatgtagatg tatgtccaac aggaaaagat
    18481 agaacagcag ttattacagg gaaactaaaa atgtttgatt ttgatgatga gcattattat
    18541 ccagctaaaa ctggtattga tatgtatcat aattataaag aagatataaa gttatttgct
    18601 gaaatgggat ttaaagtata tagaatgagc attgcatgga gtcgaatatt tccaaaaggt
    18661 gatgaagaaa caccaaatga aaagggactt caattttatg aaaatatatt taaggagtgt
    18721 aaaaaatata aaatagagcc tttagtcact attacacact ttgattgtcc aatgcattta
    18781 gttaaaaagt atggtgcatg gcgtaatcga aaaatgatag gtttctatga gaaattatgc
    18841 aatgttatct ttagaagata taaaggatta gtgaagtact ggctaacttt caatgaaata
    18901 aatatgattt tacatgctcc atttatgggt gctggtattt gctttgaaga aggtgaagat
    18961 gttgaaaaga taaaatatca agctgcccat catgagcttg tagctagtgc aattgcaact
    19021 aaaatagcac atgaagttga ttcaaataac ttaattggat gtatgtttgc agctggaagt
    19081 gtatatcctt atagttgtaa tcctaatgat gtatgggaag ctacaaagtt agatagagaa
    19141 aattacttct ttgtggatgt acaatctaaa ggtaaatatc ctaactatgc gttaaagtat
    19201 atggagcaaa aaggtattac accagaaatg gaacctggtg atattgaatt attgaataaa
    19261 tatacagtag attttatttc attttcatat tacaatagcc gttgtgttag aaccgatgaa
    19321 aatgcagatg atatggctga aggaaacata tttacatctg ctaaaaatcc atatctttca
    19381 tacagtcaat ggggatggcc aattgaccca ttaggtttga gaattacact aaatcatgta
    19441 tatgatagat atgaaaagcc actatttgtt gtagaaaatg gattaggtgc taaagatata
    19501 gctgatgaaa atggttatgt agaagatgat tacagaattg attatataag agaacatata
    19561 aaagctatgc atgatgcagt ttctatagat ggggtcgatt tattaggtta tactacatgg
    19621 gcacctattg atttagtaag tgctggtaca ggtgaaatgg aaaagcgtta tggatttata
    19681 tatgtagata gagacaatag tggtaatgga agtctaagaa gaatgaaaaa gaaaagtttt
    19741 gaatggtata agaaagtaat tgcttcaaat ggtgaagatt tatattagac aagaaatcta
    19801 tgttaaataa gaaatttgta ttaaataaga agagtataaa aattaataat aagtataatt
    19861 tttaactttt ttaactatag aaaatcaata cagaattatt ttacattgta tttatgcaat
    19921 agtatgattc acaaataaaa ttttagaaaa taaaattgtt aagaagtatg ggaagtaagt
    19981 gcaatttact ttgtaataaa agatgaaaaa ggcaagctta ggttttaagc ttgtcttttt
    20041 tattatgtat aaactatgat actttaaaat tctatgaaaa atttatttaa tagagaaatt
    20101 aaaaatttaa tggtaatcaa attctttacc aatgaatata aaaaatgtct acgtgttata
    20161 atagtttaaa ctaataaacg aaaggtagat gaatgaatat gaaataccaa catataatag
    20221 aagttgacaa agaactttgc attggatgtg ggctttgtaa aaatgattgt ccagtaaaca
    20281 atataattat agaaaataaa aagtcagtaa ttaaaaaaca agattgtcta atgtgtggac
    20341 attgtgcagc aatatgtccc acaaaagcta ttaccttgac aggatttgat gaacctccta
    20401 tagaacttac aaataagcca aagctagatt cagatgaatt actaatggca attaaatcta
    20461 gaagaagtat acgtaaattt aaagacaaag aagtttcttc agaaattata aagcaaatta
    20521 ttgaggcagg taggtatacc cctagtgcta aaaattcaca agatgtatca tatattgttt
    20581 tagacaataa aaagtcaatt tatgaaaacg aagcagtgaa attttttaga aaaataaaac
    20641 ctattgcaaa tatagctatt aaatattcaa aagaagttga gattgacgat aactttttct
    20701 ttaagcatgc tcctattgca ataatgataa ttacaaaaga taagattagt ggttcacttg
    20761 cagcatcaaa tatggcatta atggcagagt cttatggcct aggtgtttta tttagtggat
    20821 atttttcaga tgtagcaaat aattcgccaa aattaaaaaa gcttttaggt ctaaaacgca
    20881 gtaatcatgt tcttacaaca ttagtaattg gatacccaga tgttaaatat agacgtactg
    20941 cacagaaaga agtagcaact gttagatact tataagagag ggtggatttt atggaaaaag
    21001 aatgtcaaca aagaatgcaa gaaataacgt ctaaatttaa agaatgtcgc aaagctatct
    21061 ctgctatggg tgatgagact agacaattga ttttaattgc tctcttagag agtgatttta
    21121 atggtatacg agtgggagaa atcacaaaaa aaacacatct ttctcgtcct gctgtatctc
    21181 atcatcttaa gatattaaaa gaagcagaga ttgttaattt gcgtagagaa ggaacaaaaa
    21241 attattatta tcttgattca aaaaaatcac aatggaggtc actaacagag ctaataaatc
    21301 ttatatttgt tggaatacaa catattagtg aaaatgatat aagaaaaggt gattaaatta
    21361 tacttgattt aatacaaaaa gattgattta ggaactaaaa agaactaaaa agttcacttt
    21421 gagacactat cttttgaact atacccatct taggagacag ttaaaatatg taaaactgta
    21481 taaaacaatg ggtatagttc attttataat ggtttaaata ttaagaatat attattctaa
    21541 atagtatctt tctctgtttt gagttttatt tttatactca atagcttcag tggggcaacc
    21601 acaaatacat gccatacaat gagtacagtt atttttccaa actggttttt tgttttttaa
    21661 attaatatta tttaaaggac atagctctac acattttcca cagccaatgc atttatcagt
    21721 ataatgaaat cctttggcac taataattgt cttataaaaa actgtattta ttataccact
    21781 tttaattgta cctcctaagc cactaggagt aataatttca aattcatttt catttcgtat
    21841 atcagaaata atcctatata tttgtttatc tgcttcttta atcatttgtt ttgatgtttc
    21901 tttgtctgtt gtagaaaata aagctatata attctctggc attttaattt ctgccatacc
    21961 ttttaattgc cattccttgt atttacatag tttttgaata tagtttatgg attttgctgt
    22021 atctccacca caagtcatta caaagtaaac tctgttgctt cctagaaatt ttgtttcttt
    22081 tataaaatcc gtaaccacta ttggcaatct ccatgcataa gtaggacata caaatatgaa
    22141 tggtttatct gttgatatta attcatctgt tttttcctct tttataagtt gatttagtga
    22201 tatcagttca tcatttaatt cttgtgcaat tttacgagcc acatatcggc tattaccagt
    22261 tccgctaaaa tataaaatca taataatatc tccctaacta ttttttattt taactcttaa
    22321 ttttttattt attatagtaa tttattgata tattctattc aaatgtatgt aaaatttagt
    22381 agttaagaaa attataacac attagtaatt tttatactta ttggtaaaga atttgattac
    22441 cattaaattt agcaacaaga catcaactgg acttgtatat gagaagggat aataatttgc
    22501 ttatgaaaga ttataaatat aaaggttatt ttgaaggtgt agaaatattt gataatgtaa
    22561 atgaccttaa cttaaatact ttaacaacag ttagtagatt tgtgtttgtt tttttgttta
    22621 cgataactta aggaaatatg ttacctgaac aataaattta tataataaaa gtatataaat
    22681 atatatatga atgttaagat tagttgatag aatttaaata ataaatccaa tattataaac
    22741 ataggaggta tgaaaaatat gattaatgag aatttaaaaa gtctaagaaa aatcaatcag
    22801 tatacacaag aagaattagc tgaaaaactt aatgtttcta gacaatcaat agcaaaatgg
    22861 gaaagtggag aatctattcc tgatataggt agttgcatta aacttgcaaa attgtacaat
    22921 gttaaactag atgacttagt taatcattcg gaagaaaaaa caggtattat agtacctcct
    22981 aaaggaaagt ttttctttgg agcagttgta gttggtgaac gtggtcagat tgtcattcca
    23041 aaagaagcaa gagaagtatt taatataaat gcaggagata aattattggt acttggagat
    23101 gaggaaagag gattaggaat tgttcatcaa cgtgatttga taaattttat aggtcatgtg
    23161 ggagtagtac ataaagaaga atgatttaaa aaaattataa aaggaggtat gaaatatgag
    23221 taaaatagta ttttttagca ttcctgcata tggtcatact aatcctacta ttgaagtagt
    23281 tcgtgaatta gtggatagag gaaatgaggt attgtattat tcttttaatg aatttaaaga
    23341 taaaattgaa ggtgctggag caaaatttat ttgttgtgat aaataccttc cagaattact
    23401 ccctggagat gagaaaaaaa ttggtaagga ttttcctgga ttaattgaga tgatagtaga
    23461 tactactatt tctttagatg aaaaagtttg tagagaactt aaagatttta atccagattg
    23521 cattgtatca gattcattaa gtttttgggg aaagttattt gcacaaaaac ttaatattcc
    23581 atatgtatgc tctacaacaa cttttgcatt taataaacat acagcaaagt tgatgaaaca
    23641 aaactttata gaaattatac gtatgatttt tggtgtaaga cgcattaata aaaaaataaa
    23701 gcttttacaa agaaaagggt atgaagtaaa aaattttatt tctatagttt ctaatgataa
    23761 tgatacggat accattgtat atacatcaaa agaatttcaa ccaatggtag aaaccttctc
    23821 tagtaagtat tcatttgtag gtccatcagt atcaaagttg attattgaac ctaaagagag
    23881 aaaaagaaag ctaatatata tttcgcttgg tacaattaat aataaaaatg ttagctttta
    23941 taaaaactgt ataaatgcat ttaaagattc tgatgttgat gtgattatgt ctgttggtag
    24001 aagtactgat attaaaagtt taggaaatat accaaataat tttgaggtta aaaatagtgt
    24061 tgaacagatt gctatacttc aaaaaactga tgtgtttgtt actcattctg ggatgaatag
    24121 tgttaatgaa agtttatatt atggagtacc tatggtatta tttcctcaac attcggagca
    24181 gagaatggta gctaagagag ttgttcattt gggagcagga attatgttaa aagaggataa
    24241 atcagaaagt attaaaaaag cagtatttca agttataaat gacaatgaat ataaagaaaa
    24301 tgcaactaaa ctatcaaaaa gcttttataa tgcaggaggc tcaaaaaaag cagcagatgt
    24361 aatattacag attatatgta atagtagaca agttgacata taatagattt gttaaaaata
    24421 tatatacata catgtaattt aatcaaagta ctgacagtgg ggtcttttaa cctaccagtt
    24481 ggggcatata tattgatgct aaatatgagg gatgttatat tttaataaaa aagataacat
    24541 ccttttttta gtgcattata tcgagtttta aataataaat gaaggaggat taatatgaaa
    24601 aaatataaag ttatttttat agtattaatt ataggtatta taagtgtaac agggtttgtt
    24661 gtatataaaa actgcattaa accatggaaa gatgctgaaa catatattga taagtatatg
    24721 gtaaaacaag atatttcaaa agataatata aaaagtatta caaaagaaaa agccaagaag
    24781 gcaagttacg aaggaatact atacaaagtg tattacaaag atgaccctcg atatgagtat
    24841 caatattttt atagtgataa ttattatgct ctatatgtaa ataaagtaat gttacaaatt
    24901 tatgatatgg aaaataataa taaactttta agaagtaatg aggaattaaa aagtgtaaag
    24961 tatccaccaa tatatcttaa atataagcca gataaatgat ttatgacaaa atagaataat
    25021 ttttattaaa atttatttaa aaatatcact agagcgttta aataaaacta aatttaagta
    25081 tttatatatt agtatatctt actagtgttt aaacaaaat
//
LOCUS       DAYAOY010000055        25055 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYAOY010000055 DAYAOY010000000
VERSION     DAYAOY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.055"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(204..812)
                     /locus_tag="ACGI6B_002340"
     CDS             complement(204..812)
                     /locus_tag="ACGI6B_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFG9751781.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(921..3659)
                     /locus_tag="ACGI6B_002341"
     CDS             complement(921..3659)
                     /locus_tag="ACGI6B_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9751782.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3807..4598)
                     /locus_tag="ACGI6B_002342"
     CDS             complement(3807..4598)
                     /locus_tag="ACGI6B_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751783.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4634..5290)
                     /locus_tag="ACGI6B_002343"
     CDS             complement(4634..5290)
                     /locus_tag="ACGI6B_002343"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFG9751784.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5283..6248)
                     /locus_tag="ACGI6B_002344"
     CDS             complement(5283..6248)
                     /locus_tag="ACGI6B_002344"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFG9751785.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6354..6460)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6750..7694)
                     /locus_tag="ACGI6B_002345"
     CDS             complement(6750..7694)
                     /locus_tag="ACGI6B_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751786.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7805..8272
                     /locus_tag="ACGI6B_002346"
     CDS             7805..8272
                     /locus_tag="ACGI6B_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HFG9751787.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8442..9041)
                     /locus_tag="ACGI6B_002347"
     CDS             complement(8442..9041)
                     /locus_tag="ACGI6B_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751788.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9287..9883
                     /locus_tag="ACGI6B_002348"
     CDS             9287..9883
                     /locus_tag="ACGI6B_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HFG9751789.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9981..10940)
                     /locus_tag="ACGI6B_002349"
     CDS             complement(9981..10940)
                     /locus_tag="ACGI6B_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751790.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10974..11705)
                     /locus_tag="ACGI6B_002350"
     CDS             complement(10974..11705)
                     /locus_tag="ACGI6B_002350"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751791.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11698..12468)
                     /locus_tag="ACGI6B_002351"
     CDS             complement(11698..12468)
                     /locus_tag="ACGI6B_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751792.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12553..13263)
                     /locus_tag="ACGI6B_002352"
     CDS             complement(12553..13263)
                     /locus_tag="ACGI6B_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFG9751793.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13443..13595)
                     /locus_tag="ACGI6B_002353"
     CDS             complement(13443..13595)
                     /locus_tag="ACGI6B_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFG9751794.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13621..15777)
                     /gene="feoB"
                     /locus_tag="ACGI6B_002354"
     CDS             complement(13621..15777)
                     /gene="feoB"
                     /locus_tag="ACGI6B_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9751795.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15877..16098)
                     /locus_tag="ACGI6B_002355"
     CDS             complement(15877..16098)
                     /locus_tag="ACGI6B_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9751796.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(16111..16323)
                     /locus_tag="ACGI6B_002356"
     CDS             complement(16111..16323)
                     /locus_tag="ACGI6B_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9751797.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16644..19541)
                     /locus_tag="ACGI6B_002357"
     CDS             complement(16644..19541)
                     /locus_tag="ACGI6B_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9751798.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(19868..20476)
                     /locus_tag="ACGI6B_002358"
     CDS             complement(19868..20476)
                     /locus_tag="ACGI6B_002358"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFG9751799.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(20736..21281)
                     /locus_tag="ACGI6B_002359"
     CDS             complement(20736..21281)
                     /locus_tag="ACGI6B_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFG9751800.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(21399..23222)
                     /locus_tag="ACGI6B_002360"
     CDS             complement(21399..23222)
                     /locus_tag="ACGI6B_002360"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751801.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(23235..25010)
                     /locus_tag="ACGI6B_002361"
     CDS             complement(23235..25010)
                     /locus_tag="ACGI6B_002361"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751802.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         8268 a         4348 c         2447 g         9992 t
ORIGIN      
        1 ataaaataca caaaaaaatt aatttttata gctcatttcg ccaaaatata tctttcttta
       61 tatcattaaa ataattataa atttattaaa tatgtttgta attctataaa gttattttta
      121 tcaaactaaa aattctataa aaaaataaga tacataccta taaaatacct tcctatagat
      181 acatatctta cctatataaa aacttattct atataaaaac gccataaaaa atcttttgct
      241 tcttctgcat aatctatatt tatacgttta ctagttttta tatctaactc atctttacta
      301 aatacttttt ttcctttatc atcataataa aaatcactta tatataattc ttcacccatt
      361 atactcttac tatttagact tctatctatt tttaaagctt tgcaaagttt tcctggtccg
      421 ttagtaatat tttttacttg atatttactc aactcagtgt aagctttgtt gtatctattc
      481 atagatatct catctaaaga agttataggc tctacccctc ttattaatac acactctggc
      541 acattttcta tatttgcaga taaattcaag caattataca taccataaat taaataaaca
      601 tatatatgtc caccatctaa atataatggc ttagttctat ctgtcttttt attgccatat
      661 acatgtgctc ctttatcatt tacacccatg tatgcttctg tctctatgat tttggtaaca
      721 attactttat tttcatattt ccttattaga tatttaccta atattgattt ggctaaatca
      781 attccatttt ttctaaaaaa atccttttcc aaaatttcac ctcataaaat ttatttaaaa
      841 cgcatcaaat acaactaaag taataaataa aattaactct tattaatgtg atatatataa
      901 acaataaatt tagacattta ttatttatat atatcaaaaa attctatctc aaacttttca
      961 atattatcat tgtaatttga attatcatat acttcttcaa caacctcttt aattgggaat
     1021 ataactttta cattagttcc ttctcctacc ttactatcta tgtaaatctt cccattttga
     1081 atttcaatca atgattttgt aaggcataaa cctataccac ttccaaattc atttgatata
     1141 ccactgtttt caatttgttt aaatcgatta aatatatcct ttaatttttc tttgtctatg
     1201 cccactccag tatcttttat aattatctct actgtttcgt cttttttata tatacctaca
     1261 aggatttctc ctccttctaa agtaaatttt atagcatttg ataatatatt tagtattatt
     1321 ctttctatcg attctgggtc aagccctacc ataacagttt cttcatctgt atcaaaaatt
     1381 aaatctattc ctttagactt agcataacta ctaatagatg taactattct ttctgttatg
     1441 tcaataatat caaaattaac tgctttaatc ttatatatgt ctgacttaac ttgagaaata
     1501 tttattatat tatctattaa tctcaataat ctaaacatat tttgtttagt tatttttata
     1561 tgttctttta gtatatcctg aaaatcaatt atttttcctt ttttataaag gttttcaata
     1621 agttgaactg aactatatat attatttaaa ggtgtcttaa gttcatgaga tatattagca
     1681 taaaactcca tcttcatttg gtcatactgt ctttgtttta ttaatgcttt tttaagtctt
     1741 tcagactctc tttgcacact taaatcttta ataattctca ctctacttct tttctgtcca
     1801 aagtataatg ttcttgatat catttttact tctttaatgt gattattttc atttactata
     1861 tggatactct tctctctttc tcctatattt aaattgttta tgtatttttt tactaatata
     1921 tttctatatt tttgtgttgt aactcctaat acatcactag aaattccgaa caactctcta
     1981 aaggctctat ttgtatatag tactcttgat gtacttaaat cctcaagaaa aatagcctct
     2041 ggtataacat ccagtaattt ttcatatatt attttttgtt catttactgt tctaagaata
     2101 tttatctctt tagttatatc tctaaatgta agcaatttaa agagtttatt atctgaccaa
     2161 tagtctccaa ctctaacttc ttcaacatgt tcttcacctt ctgagtcaga catcacaaaa
     2221 ttaactatac agtttgctgt gtcattgtaa cttactattt cagctttctt ttcttgtgtt
     2281 agatttaatc ttttaaattc cctattgtca aagaaatcta tgttaaaagt ttcaaaatat
     2341 tgttctaatg tcatatcgac taaatcactt gaaacacatt taaaactctt ttcatatttt
     2401 ttatttatac ttattacctt attcttcttt atgccacctt cagcacttct tacaagtata
     2461 ggagatggaa tatttagtat aatgttttca tagtctttat ttatatttct tatttcttca
     2521 tctattttga tttcatttgt aatatcattt aaacttatta agtaattgct ttcgtcttta
     2581 ttattactta aacttaacaa ataaatttta tcatttaact ctaccagttt atcaattata
     2641 cttatatcgt tatcaaattt cttcaatttt tcagcaactt tttcatcttc ctttataaaa
     2701 gttaatattt tataataatt attcatatcc tttgaaaatt catctttaaa tttattattt
     2761 gtgtaactta tattaaaatc atagtctgtt actatccata catttggtat tgtatccaga
     2821 gcttgtctaa gtaattcttt tctctggtct atgtagtttt ttaatgttct ttgtataatc
     2881 atatcctttt ctattgctaa atttatcttt cctaattgct ctgacttttt acttaatttt
     2941 ttattgattg aatcaagttc ataatatgga tacactactc tattattaag actgtataca
     3001 tatacataat aagtatggtt acataatagt aaatatatta ctcctattaa tctatctgaa
     3061 tctactatat taatcttaga aattgctaca aaacatataa tagttgtaaa aaaaacaaaa
     3121 tatgttaaat cttttattaa cttatagtgc tcaatgttac tatcaagttt attcaacaaa
     3181 tatagtaaat taaacataga tatacaaata ttaattaata taataatcat attccagtta
     3241 atatcactaa aaatatttct tataaagtaa tctaatatca caaaagcact ataaaaaatt
     3301 agtattgggc ttattcttag tttttttact ttagaaatgg ttaagtatat aaaagtagac
     3361 atattaatta tgcttaagtt aagcaataca atttggaatt tttgaatttt taaatttact
     3421 actcctaaaa tacttacaaa aactaataca agacacccta ctttcaatat atttataaat
     3481 tcatctgttt taaagctact tatataaact atagaatatc caaatatcca aagtaaaatt
     3541 attctataca tatttataga aactgtatac aaatttatat ttaaaagtgc aataaccaaa
     3601 aataacccat tagctagtat atagacctta ttcttactta aagttccttt agattccata
     3661 ttccccacca cttcttcatt tttatatagt tttacctata aatatgttac cacaattctt
     3721 ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag taaatatttt taattttact
     3781 cattttgtag attttgtata ttcagtttaa aaagctggaa ctatacttcc tttatattca
     3841 tcttgtataa attttttaac ttctttacta ttcatagcct ctgataaagc cttaatctta
     3901 tcactatctt tattattttc tctacaagct attatattta catatggaga atcacttgat
     3961 tctattacta ttgcatcctt agtaggattt aagttagctg ttaaagcata attagaattt
     4021 attactgctc cgtcaacatc ttttaatact gttggtaatt gctctgcatt catttctttt
     4081 attactatat ttttaggatt tttagttata tcttttttag ttataagctc tccatctttt
     4141 acttctatca atttattttt tgctaataat ttaagtgctc tagctccatt agttgcatca
     4201 tttggcacag ctataacagc tccatctttt aattcatcta atgacttaac tttttctgaa
     4261 tagaatccca ttggctctat atgaacttta gatgtataag taagtttata tcctttctcc
     4321 ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt tagcatccaa agacccttca
     4381 tctaaagctg tatttggaag tacataatca tcaaatattt ttacttctaa atcatatcct
     4441 ttttctttta acaaaggttt tgcaacttcc aatatctttg catgtggatt tgatgaagct
     4501 cctactagta tttttttatc ctctttgttt gaacaaccta ctgctgatat tgctattgct
     4561 gacaccaatg ctactgataa taatttcttt aatttcattt ttactccccc tctttaaaat
     4621 gtttataatt aatttatttt agtttcttat aagccagatt tcctactccc tggattatct
     4681 gaactaatat tataagtgct attacagtat ataccattac tgctgtatca aatctttgat
     4741 atccataaat aagtgctata ttacctaatc ctccagctcc tactgcacct gccatagctg
     4801 tataaccaag tatagatatt attgataatg taattccaga tactattgaa ggcatagctt
     4861 cctttatcat aactttaaat actatttgtc tttttgtagc tccaaaagat tttgctgctt
     4921 ctatcaatcc tttatcaact tcatttaatg agctttctat aattctagcg ataaacggtg
     4981 ctgccccaat agttattggc actattgcag cagtttcccc tatacttgtt ccaacgatta
     5041 atctagttat tggaatcatt gcaactataa gtatcataaa tgggaaactt ctaaatatat
     5101 ttactataaa tcctaatgca ctatttattg tgctatttgg ttttaatcca tctggttttg
     5161 taactactaa tatgattgct aatataaatc ctaatattgt tgccactata gttggtacta
     5221 taaccatata caaagtttgt aacaatgcat ttggaaatag tgttgttaaa aagtcaatta
     5281 aactattcat ttatcatttc ctcccatctt atgcccttac tagttagata ttgttttact
     5341 tgctctccac ttttatctga aatgtttata ataagtgaac ctaaaatatc atctttaaat
     5401 tgttctaatt tcccaaatat tatagacaca tctatattta gttctcttgc catagtcgta
     5461 ataatagctt cattagaaat atctttaggg aatagtatct ttatatttgt tccttttggc
     5521 aatattatac tttcttcacc tattaatttt ctaagtcctt tagtatttct taagaatatt
     5581 tcttctgtat ctcctacctc taatacttct ccattttcca ttattgctac tcttccacaa
     5641 atctgcttta taacttccat ttgatgagtt acaactatta ttgttattcc caatttttta
     5701 tttatatctt caagcaatga aagaattgac tttgtagtat ttgggtctaa tgctgaagtc
     5761 gcttcatcac aaagaagtac ttgtggattt agtgctaaag ctctagcaat tgcaaccctt
     5821 tgtttttgtc cgccacttag atttctgggt ttatcatttt tcttttcaga tattccaact
     5881 acttctaata actctaaaac tcttttttta atttctgcct ttgagtatcc aaaacattct
     5941 aatggcaatg ctacattatc aaatacagtt tttctttcaa gcaatgaaaa gtgttgaaaa
     6001 atcattccta actcttttct taaatctctc atttgctttt cattcaatga ctttacttct
     6061 ttatcagaaa caagtacact gccttcttgg tattcttcta atccgtttat acatctcagt
     6121 agtgttgatt tccctgcacc actatgacct attattccaa aaatttctcc agattctatt
     6181 tcaatactta catctttaag cacttgaatc tttccataat acttattaac attttttatg
     6241 ctaatcataa aatttcctcc catatattct gctcagtttt tatgtttatc ctctcttatg
     6301 tattacaaca aaataaaagc ccgagtataa actcaggctt agaaagctat ttatactcat
     6361 ctatcaacta tattagttgc aggatttagc accaatgtat ataaaatata ctggttgccg
     6421 ggtttcaaag ggccagtccc tccaccactc ttgataagac gttatttaat ttaattctat
     6481 tatcatttgt tgctatgtta tatatgatat attcattaat agtatttgtc aataaattta
     6541 tttaaatttt ttatttttta ttatcgttaa ttaactattt ttaattagtt ttctagctcc
     6601 taacacttta tattttaatg tctatataac tatagaacat cattataaga aataaattaa
     6661 ataacatcaa tctatctaaa tcatctgtaa agtaaattaa gaaaattaac attttatcca
     6721 aactctatcc tactattaaa taaaataatt taaatacaat gatgattata tagctctagt
     6781 actttagact ttatcaaatg tttaagttct ataggttcta ttattgtagc atttttccca
     6841 aaagttaaaa aatattcagc cacccagtta agtgtagatt ttggtatata catatctatg
     6901 tatccacctt tagacaagat tttcaaccct cttgccaaca aaaattctgt ttcacaacgt
     6961 tttactccct catctgtcaa attaatttta atatgataat ctgtacctac ttccatattc
     7021 ttaagataat catgtataga agataaaacc ttatatttct tacttgataa gttttctata
     7081 ctcaaaatct tcacaattct gtctacacga aattccttaa tagattctga ttttatacag
     7141 taagctggac aataccaaag tccattcatg gcatacaaac caataggaac aatggtacgt
     7201 acactatttt tttgctttga tgaatattca attttaattg taaattcatt cattactata
     7261 ttaaacaatt ctttcaataa tggtgttgaa cagtgcctat ctggtgtcca aaaaacaaat
     7321 ttacgcctta tattctcaat attatattga atatcatttg gtatacaatt taaaaatttt
     7381 tttaaaacag aaatagtttc ttgctcaaat ggcaaatcat tataatattc tagtgactga
     7441 tatgcaaaga atatagattt tacctcactt tctgaaaatg caatcggtgg aagtatacgc
     7501 tctttaagta catgatatcc accagccgct ccaacttttg aatataatgg aaatccaata
     7561 ctctccagca cctgtaaatc tcttagtata gtacgttttg atacagaaaa ttcttctgct
     7621 aattctcctg ctgtaaaact tctttttgca tttattgtta tcatcagttc aattaaacgc
     7681 tcagctcttg acatatttat actcctaatt taaatatagt gacaataatt gtcactatta
     7741 tatgataaga tattcttgca tctaaagcaa taataatagt aaagaaagaa gggattgata
     7801 ctttatggaa aatgatacta ttaaggctga tgatattctc aattattgtc tatcaaactt
     7861 agatgatgtt gtactaatgg atagttgggg ggaacgagca atttactaca atcctaatgg
     7921 tgttttaaag cgaggggtat atgttcttac cattaaggaa aaagacagta ataatgataa
     7981 aggttcgtta gttagtcgtc caaatgtata ccgtgtgaat ataggattaa aaaaagaaac
     8041 ttttattgaa atgtttggat atattccaaa gcgtccaggt gtaggtcaaa tagttgatat
     8101 ggattttgat tttacaaaat tggacacaat catgccacat cctatctact catggatggg
     8161 atggatatgt gtcctaagcc ctactgaaaa gacatttgag aactttaaaa ctttaatagg
     8221 agaatcttac aattttgcaa aacaaaaatt taaaaagaga aaaaacaagt aaaaacgtgt
     8281 gaaaataacc tctaaataaa ttataataaa aatattaagg ttacaagata aaaattaata
     8341 tcttaataac atgcaaaaaa gcgaagccaa aaaatgtatt atttataagg cttcgctata
     8401 tctcaaataa tttatttcaa atttcaaaac tatttattct attatttctt attatttcct
     8461 gttattccta atctatcatt catatctttt atcatttttc taaaaccact acctatatca
     8521 tttccaacat cttcagttcc atgaactaat tttataaagt cattttgacc ttttttcata
     8581 tcttcatcag ataccttatc taatttttct attctcttat ctatatcttt tactaatctt
     8641 tccccttcag cgattaggtc atcattcatt ttctttaact ctgtatcttc tgttttacca
     8701 tcttttttaa gagatgttac aaatgattgt attttatctc tactatcttt atatgcagcc
     8761 tttacatcac ttagatattt ttcatttcct ggatattctt tatctttaaa ctgattattt
     8821 atactttctg gagtatcata aatagaatat ctatttagac ctgctatata atcattatag
     8881 cttgttgaat aataattcat atatccttca ttattattta cagattcatt aggatttttt
     8941 cctgtatttt cacttggttt ttcattattt ttagtacatg cagttatacc tatagctaat
     9001 actagtgcta atcctacaaa taaagttctt tttaatttca tttttattac cctcctagat
     9061 gttttatact ataaaaaatt ttaataattt tctttcttaa tttagtattt ctatattttt
     9121 atattttatt tactaaaatt aaacaagtta taatttattt ttttggaaat cataatgaat
     9181 aaataattaa ataaaaaaat tataatttta tcattgatat tgattatcat tcatagtata
     9241 atctaattac ttgatattaa ttttcatttg aaggaggtta tcttttatgt cttgttgtaa
     9301 ttcacaattt tgttgtaaaa ataaaattga atcatcatat ataaaatggc ttttagaagt
     9361 tttaggtcca gtctttttag gctctaaacc tgctgaaata ataaatattt cgtttactga
     9421 tgtaaataga gaggaaaaaa ttaatgatat ttataaatat ctatataagt gtaagaaaat
     9481 agattttata gttattgata aagaaaaaaa gggattaaaa atcttatttg taaataaaaa
     9541 agcattatca gaaaaattaa aatgtaaaaa aacagtaaac tttttaaagt ttttaggata
     9601 caaacaaaat acgaatgtta atgcttattt agagcattta gtagataaac ttaagagtga
     9661 tgtttttcct gatgaaatag gtattttttt aggatatcct cttaaagatg tgattggatt
     9721 tatgggatat agtaattatg aagtttctat gattaaatat tggaaagttt atggtgacac
     9781 aaaacaatcc gaggacacat attcgaagtt tttacttcac cgtaagaaaa tgagaaaatt
     9841 actagactac ataagtgtag ataaaatagt aagctgtttt taattgtgtt tttgtataat
     9901 aataaaaata caccctcttt taccatacag tttccataat aaataagggt gtattttcca
     9961 ctttaatatt ttaaagtatt ctaattttga ttgtacaaca tatctttaat atttattttc
    10021 ttttgttgca ttccattaga catcaaaaat tcctgtgtat cttctaattc ttttatatcc
    10081 ttgtccgtta ttttagaact aaagtcatac caagaataca tttcttttgt ttcatctaaa
    10141 gataaaccaa cttctttaga tacaacatcc atcacttcat ccttgttttc attcatatat
    10201 tttaaagttt cttcatgaac cttcataaat ctttttacta attcaggata tttttcagca
    10261 aagtcatcac ttacagcagt aactattatt ccacttacta atccttctcc atttacaatc
    10321 attttggcac ctgaatttat cgttttcaat gccactggac ctgcaagcat tgctacatca
    10381 gcacttttac tttctaatgc agaagcagca ctaggaatat ccatatttac atattcaaca
    10441 tcatctatag tatagccttc tttagcaagt gcagcaatca acagttgatg aagtatagtc
    10501 ccttttggac ctacaatttt tttacctttt aagtctttta tagatttaat attatcatta
    10561 ttagttataa gcatatatcc ttttggtgaa cggctataaa tattcgtaat tttaagacca
    10621 acaccatttg atgcagctat tattgcagac gttcctccta atgcatgtaa aaagtctagc
    10681 tcacctgctg ctaatgcttg agtttgctct ggtcctgttg tcaattcatg gaaattgact
    10741 tttatattgt cctttttaaa ttcctttcca aataaatcat cctttttttc taatatacta
    10801 ggaacattaa gtggtgattt tacataagtt aaatttattt ccttaggtaa actagcctct
    10861 ttttttgcag gttcatcttt cttatcattt gttgttgaac atcctacagt cccaaccaac
    10921 accattatta aagatgtcat tactgctaaa atctttttgt ttcttttaat caattaaaac
    10981 tcccccaatt cccttaatat ttcttttttc aagtttatat aatagttttt agttaaatct
    11041 ctattatatt catcttcgac actaaattgt ttaatccttt tatttttact aaaaacaatt
    11101 attttctttg ctatttccat agcttcttca atattgtgag ttacaaatac tacacctttt
    11161 ttagtatttt tatggatatt tacaacttct ttttgcattt ttcttcttgt gaagtaatct
    11221 agtgctgcaa atggttcgtc catcagcaaa atatcgggat tgaaggaaag tgctcttgct
    11281 atacttactc tgtgagccat tcctcctgat aattcatttg gatatgagtt tttaaacttt
    11341 tcaagcttca tcatttttaa atacttgtct aaatcaactt tatttctatt atctttttca
    11401 gtatgtaaaa gtatattttc acttacattc agccatggca taagtctact ttcttgaaat
    11461 accattccta cttttggagt acacttttta tcattattaa aaaaatatac gtttccacta
    11521 gtagcatttt caagccctgc taaaattcta agtagcgttg ttttcccaca tccactttca
    11581 cctaatataa cagttatctc ttcactagat atatttaaag atatattatc aagaactaaa
    11641 tgctctttat tatcaactaa atatttctta tatatattct ctagcaaaaa tccacttcta
    11701 accatttaaa tcaacctgct tccctttgct aaattttgtt attatctttg aaaatagata
    11761 atctgaaagt atacctaaaa atccaataac aaaaattcct accaatacaa catcagttct
    11821 agacatatct tttccatcag aaatcaaata tcctagtcct gaagaagctg caataagctc
    11881 tgctccaatt atagccctaa aactataccc aagccctaaa ctaagtccaa caacaatatc
    11941 cagcatagaa ttagggatta ttattttata aaaaattctc tttttagata aattaaaaga
    12001 tttacctact tctattaatt tattgtcaca attttttata cctttcaatg tatttaaaaa
    12061 tattggaaaa aaggatgcca aaataataat tataatttta gattcttcac ctataccaaa
    12121 ccataaaata agcattggca ccaaagctag tggaggtgta cttcgcataa attcaaatag
    12181 tggtttgaaa tattcataca tttttttatt tgctccaaat aaaactccaa gtggtatagc
    12241 catcaatgaa cttattaaaa aacctattaa tatccttctt acactggcat aaacatttaa
    12301 gaaaatagaa ccgtcatata tcatttttat aaaagtatct aagactattt caggaggagg
    12361 cagaatatag ctactccata tacccaaaac acatactatc ttccataaaa ttaaaattat
    12421 taaaaaaata taatatcctt taagcctttt atgaattttc tcaaacatat aaaactcctt
    12481 ttaacatatc tatccctgct acccttttta ttttttaata tcttaaataa taattaagct
    12541 atatcataag atttaatcta tgccaagtct taatccacct gaactcattc tatcagaagg
    12601 tgtagaatct gtttttaagc cagacaatct ttcagatggt tgtataatga ctatttgtcc
    12661 aggttcaaca aattccatat gataagattc tcctgaagtc tgaccaataa aagtcttcca
    12721 tgatagtttt gatactttag gatatggttc tcttccagtc caagctacta atgcatctgg
    12781 gtcaacacaa caaggtcctt caagaattag aggattacca tctgttatta tagcaacatc
    12841 ttgattttga cctgttttat ttactaaatg tgtagtaaaa agaccttttt gagatattac
    12901 ccctgaacca atcaatttta cactgtaatt taattgttca gaaaatgcca ataagttttc
    12961 actctcaaca taaatggaat cacctgggtc aattgatatt acatctacat ggtaagcatt
    13021 ttgagctagg taaatttcac cttcaccttc aaccaccatt attggcattt gttctcctgt
    13081 caatgacctc tgaacatgac ctaataatgc tcttcccaat ccaccgccat tacttggacc
    13141 taacagcaat ttttcaaaat taaaatttcc tttaaaggct accatagcac ctttttttgc
    13201 aaaaaattta ccacttcccc ttgctataca cataagctcg tttgtaacat caaatttaaa
    13261 cataatattc acttatattg cttaatttag caataatccc tcctaatcat atatttatat
    13321 ataattatac agtaaaatta cagcatagtc taacctactt caaatatgaa atttgttatt
    13381 tatacaaaaa ataagaacct gataataaag caatttaact ttttatcagg ctcttatcct
    13441 atttatctct tagcgctatg acaactctta ctacttgagc atccagaaca accacatcca
    13501 caactgctac cattcttttt tgaattttta accatataca ttacagctag tgccattaat
    13561 actactacaa tagctgctat aataaatgta gccatattca tcactcctta tttatatgaa
    13621 ttaatttttc atataactta atttacccat tttttctttt ttcatatttt tatttgaata
    13681 agttaaaact acaaagacta ctgctactgc tataaatata gatattattg ccccagcaat
    13741 acttcctcca gtacctaaaa ctaaacttcc aacttgattt ataagaagag caattatata
    13801 cgctgttaaa gtttggaatc ctaatgtaat ccatgtccat ttccatgaac ccatttctct
    13861 ttttatggct ccaatagctg caaaacaagg tgcacaaagc atattaaatg ccataaatgc
    13921 aaacgcacta gctactgtaa acatacttgc aactgaagca agaagtgttg ggtcttgctc
    13981 tgttgcatct gatataccaa ataaaactcc aaatgttcca actacatttt ccttagctac
    14041 tagacctgta actgttgcta ctgatgattg ccaatttcca aatccaagag gagcaaatat
    14101 tggtgcaact atatttccta agctagctaa tatactatct ccagcatcta ccatttgtaa
    14161 tgaccaatta aatgattgta agaaccagat tactccacaa gcaacaaata taattgttcc
    14221 tgctttgatt ataaatgctt ttcctctatc ccacatatgt attaaaacac cttttgcact
    14281 tggaatatga tattgaggta attccataac aaatggagat ggttctcctg caaataaact
    14341 agttttcttt aatataattc cacaaatgat tatcattgca atacctaaga aatacattga
    14401 tggagctacc catgaagcac ctccaaataa tgcccctgca aataatgcta ttattggaat
    14461 cttagctcca catggaatga atgtagttaa cataatagtc atttttctat ctctatcatt
    14521 ctctatagta cgagttgaca taactcctgg aactccacaa cctgaactta taagcattgg
    14581 tataaatgat tttcctgaaa gaccaaactt acggaaaatt ctatccatta taaaagctac
    14641 acgtgacatg tacccacaat cttctaatat tgataataat aagaataaaa gcatgatttg
    14701 tggtacaaat cctagtactg ctccaactcc accaattaga ccatcaacta caagtccttg
    14761 taaccactct gctacattta aagaagctag aaaattactt acattgcctt gaataatttc
    14821 tccaaacagt acatcatttg tccaatcagt tgctatagta cccaatgaac ttactgctat
    14881 atagtaaact ccccacatta taagtgcaaa aataggtaat gctagtatac gatttgttac
    14941 aatcttatct atcttatctg atacagtttc cttacctttt cgattcttct ttattatact
    15001 tgaaattatt gaactaataa attcataacg attggctgtt attatacttt cactatcatc
    15061 atccaattca tcttcacaat ttctagttat ttcttcaata ctattcaata ttgttttaga
    15121 aatatcaagt ttttgtataa cattttcatc tctctcaaat agctttatag ctaaccagcg
    15181 tgtttctaca tctatatatg gagtcttttc ttctattatt ttttcaattt tttctattgc
    15241 atccttactt tcatcaacaa agggtaattt gaaatttaat ttattgttac tacttgcaag
    15301 ttcaactgcc ttttcagcag cctccataat tccttgacct ttaactgctg ttacttctac
    15361 cactgggcaa cctataactt cagatagttc tttaatattt atcttatcac cattcttatt
    15421 tacaatatcc atcatattaa gagctataac ggtcggtata cctagctcta aaacttgagt
    15481 tgtaagatat aaatttctct ctatatttga tgcatctaca atatttatta ctgcatccgg
    15541 cttatcatct agcataaaat tacgtgtaac tacttcttct aatgtatatg gagataaaga
    15601 ataaatacct ggtaaatcta ctatttccac atctttattt ccttttaatt ttccaccttt
    15661 tttttcgact gttactcctg gccagttacc aacatattgt gatgagcctg taagcccatt
    15721 gaacattgtt gtcttaccac agttaggatt tcctatgaga cctattttta ttgacatttc
    15781 cattccctcc attattattt aaatacatca ctgtttgtga tacagaaaat cattctcatt
    15841 atataattac attaacccta ctctaattaa tctttattat tccacaataa ttttttctgc
    15901 atctgactta cgaattgata gttcataatc tctgactgtt acctcaattg ggtcaccaag
    15961 aggagctact tttcttataa atatattaac acctcttgta atccccatat ccattatacg
    16021 tcttcgtgta gctccttctc cctctaactt tttgactttt acagtctctc cacacttaat
    16081 atccttcagt ctgttcatgt cattacctcc ctatattatt attctacttg ccattttttt
    16141 atctaatgca actctagtat cttttacgtc tattattaag ttaccatcta tcttagaaac
    16201 tattttaaca tttgtaccca ctacaaaacc tatactattt aaaaaagtcc tcgtcttatc
    16261 acttcccgaa attttcttta taactacttc ttcacctgta tttgataata tcaatggcat
    16321 catataatat ccctcctctt aaagtaattt aaatctacaa gcaatcgtta tcttatatat
    16381 aaattacaat accttaaatc ttaatctatt aaaataacat cctatttttt ccttcttttt
    16441 acaattccta attttgattt aagtctatca caatagtata ttttttgcaa gtgattattg
    16501 tattcttttg attttttttc tcatttgatt tattttatta ataaaatcgt aaaaattaat
    16561 acaaaaataa agatgctcta aaatagtata ctcaactaat attaaagcac ctttattata
    16621 tatgtcatta tttacacatt gctttattct tttttaaaag cgattttttc aaactctttt
    16681 acaggcattg gcttagaaaa caaatatcct tgtaccatat cacacccaat ttgctttaga
    16741 aattctactt gcgaaatagt ctctacacct tctgatatta ctttcatttt cagttccttt
    16801 gccattttaa tgacattaga tataacaatt ttttcattat tactctcatc tttttctgta
    16861 aaaaatgctc tatctagttt taatatgtca acaggcaatt cttttaaaag attaagagag
    16921 gaatatcctg taccaaaatc gtccatagag attacaaatc ccttttcttt caaattgttc
    16981 attgtatcaa gtaatccttc tatattatct aatactgctg tttctgttag ctctaattct
    17041 ataagtcttg tcggaatttt gtatttatct acaattaatt taaactcctc tataaaatta
    17101 ctatttacaa aatgcattcg agacaagttt acagatatag gtactacttt ttgtccttca
    17161 tccatccatt ctctaatctt tttacacact tcctcaaata catagaaatc aatatttaca
    17221 ataaaaccat tcctctcaaa tacaggaata aattctattg gtggtattag acctttttgt
    17281 ggattatccc aacgaacaag agcctctgcc cctataattt gataatctga aaggctatat
    17341 ttaggttgaa aataaacaat aaattctcca ttattaagag catctaccat tgaattttca
    17401 atttctttct cttttaatat ttttttgtgc atttccttat cgtaaaaagc aaatgaattt
    17461 ttatgaccac cttttatagt ttttcttgca gtattagctc tatccataat agtatttatg
    17521 tcattatcac ttttacttat tttatatata ccacaatcta aaatcaattt ataatagttt
    17581 attttaggat tactaagtat ttgaacctta ttgtaaatat tgtttaacct tgttttaata
    17641 tcatcatctg tcttatactt aataagaaga acaaaatggt cagcagaaac tcttgcaaaa
    17701 atttcatctt cctttaattc acttgcaata gtatttgata tatgtatcaa agtatcattt
    17761 cccatatcat atccaaacat atcatttata tatttaaatt tgtcaacatc tatataaaac
    17821 aacgcatatt cttctggatt attcttagca aacagtttat tagaatttat tttaaattta
    17881 tctatactat ctgctcctgt caatgggtct gtatatgcta tcttccttag ataatcctct
    17941 ttcttctttt tattataaat tatatattta atagcaatta agaataaaat acatacaaaa
    18001 gaaataattt gtattgaata ttctttaaaa atcacaccaa atggtacttt ataaggtttg
    18061 tcaatagtat tagaatatat acaatcactt aattcttctt cagtaatttt gtctattgat
    18121 ttatttaaaa tagacactaa tatagggtca gttttatttg atacccctat acgcatccca
    18181 tagcttgtat ctaaaacaga tataacagat aaattctcac tctttccttc tcttaaaagt
    18241 tcatcaaact gataagaact aagtatcatt aagtttgctt taccttcatt tatagcgttt
    18301 agacaacttt ctacactctt ataagatgtt gtcttagcat tttcaaaact cttttctata
    18361 taagaatcaa tagacttata actactagtt aaagcaatat ttaaatcatt attgagattg
    18421 gtatccaatt ttcctaccat aatcattgga atatttatgt atggatttgt caatttcata
    18481 ttaaatcgtt ttaccttatc attttcattg attgagccac atagcatagt tgccttacca
    18541 ctttttatgt aatcaagact ctcattataa ttttttgttt taatatattc aaattttata
    18601 ccacattttt ttgatataat tgacattaaa tcggaactta tacctttaaa tgaattgctt
    18661 tcttcatcat aatactctac aggagaccac tctgaatcat ataccatagt aagttttggg
    18721 tttgctttaa taaaattcat ttcttgccga gtaaatgcta tggaattatt gacctctctt
    18781 ttgaaatatt ttttatataa ttccaactca taatacatat catttatttt taattctttt
    18841 aaagcataat ctaattcttt tactaaatta ggtttatctt ttgcagtagc aaaataaaaa
    18901 ggttctactg aaaatttgct aactattttc attcctttag catttataat acttccacaa
    18961 acaaatgcat ctgccttttt ttcattgacg gactcaatta attcatcaat agaattacag
    19021 tatatatatt caactgaaaa attattttct tctctatact tctcaaatgc agtattatga
    19081 aagtttcctc taagtacagc tactctcatt cccttaaagg aacttatatc ttcataaaac
    19141 atattattgt tactatcctt tgtatatagt gcactatatt catatcctga acttagttta
    19201 ggaaaattaa atagtttatt tctttcatca ttcttctgta aaggtccgag taagtctatt
    19261 tcaccctttt ctagtctgtc taaacattct tcccaagacc cttttacaaa ttcatattcc
    19321 catcttgtat attttgagat ttctcttaga taatcataac catatcctgt ataagaatgc
    19381 tcttttgatg aggatattat tccgtagtca tcacaatatc caactcttat tttttttgaa
    19441 ttttcagact catcagcaaa gcatagtata ttgttaaaac atgatataat aagtaaaaat
    19501 atcaaagaaa gtatcaagaa gtgcttttta aatgctttca tatttaaaaa cctccaattt
    19561 gccaacttac tttaacttat ttcgttttct agttgtaact acttttattg taatggtaaa
    19621 tatatcaaat tgcaatattc tcatataata ttcttattca actataatga atttattatt
    19681 atctatttaa atacactatc taatattagt ttcttataag tcataaaagc acaactaaca
    19741 ctataattct agtgattaat tttatttcaa aaaatataat aacactacta atattttaaa
    19801 tatcaatagt gtcataaaaa cttttcagat taattaaatc tttctatata ttttataata
    19861 atctaaatca tgctaaatat ttagacagaa aacttttttc tttaatagta aatacttttg
    19921 tttgaatatc agaataatta tttgaatatc tatctataaa accatgattt gcatctaaaa
    19981 tctcaatttg gatattttgt ttcctagaaa gttggtaaga aatagaatca acatcaaatg
    20041 aatcgtgctt tgcaaatact aataaggtag gacattttgg aattacatcc atgtaatctc
    20101 taatccgaga gccataacaa caaattatgc catcacaaaa agaatcttca ctgcatctcc
    20161 atgcaatagt tgctcccacg ctatacccag caacaaatac atatttatac tctgatttta
    20221 aatcatttat aagcttttca acttttttat aaatatcaaa accaacatta tttataaaaa
    20281 aatcatatgc ctcttggact tttgaatatg gaaaaactct tttttctccc agaagttcag
    20341 gacaaaatac atcatatcca tatttataat acttttgaca aacatcttct ataaagctat
    20401 taatcccata gatttcatgc aaaacaacaa tagctacatc acttttatgt atatacttta
    20461 tcagattatc caccaccttt acaaaacagc aaatttttta tacttagttt aatatattct
    20521 tctttacatg tcaatttact tttctaaaat caaatttatt tttggattaa tcatttattt
    20581 ttaaaagttg tgcattatta ctattaaatt attctctatt attaaaaata taatcgttag
    20641 aaataaaaaa taaagtacaa ataattaaaa aatatctcac tcataacttt aaaaattata
    20701 agtgagatac tcctaaaatc tactttatat taaatttatt ttccaaaata tctatttaat
    20761 aatcctaaaa atgctttacc atgtctagct tcatctttac acatttcatg tactgtatca
    20821 tgtatagcat ctaatcctaa ttcttttgct ttcttagcta attttaattt tccatcagtt
    20881 gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc aactacaact
    20941 tctccaagaa gttctgcaaa tttagcagca tgttcagctt cttcaagagc tattctctta
    21001 taagcttctc ctacttctgg ataaccttct ctatcagctt gtctactcat tgctaaatac
    21061 attcctactt ctgtacactc accaacaaag ttagctctta atccttcgat tatttcttca
    21121 tctacacctt gagctactcc tattctgtgc tcatcagccc aatttttttc gcctttcatt
    21181 tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc agcatctcct
    21241 tcatgtatat atccacatac tgtacaaaca aatttcttca taattacttt cctcctaaat
    21301 tttatatatc aaattaaatt ttttatttat taagttagtc tatatatacc cgcaccatat
    21361 acctttaaac aattttttta aaagtttttt ctaaatactt attttccctg tgtcaataat
    21421 atgtttctat aataatgtcc ctttaatttt atcaattctt tatgatttcc tgattccaca
    21481 attctaccat tttctaaaac tattatatta tcacaatttg atatggtact taatctatga
    21541 gctataatta tacttgtctt tccataagtt aaattattta ttgcttcttg aattttagct
    21601 tctgttgcta tatctatgtt acttgtagcc tcatccaaaa taagtattgg agcatttttt
    21661 aatactgctc tagcaatact tattaattgt ttttgacctt cacttaaatc ttcaccccta
    21721 tccgataata cagtactata tccttcacgc attttaacta taaatttatg tgcatttgct
    21781 attttactag catatataac ttcttcatca gtagcctcca gatttccata ccttatattt
    21841 tccataactg tatcattgaa tatataacct tcttgtggaa ctactccaaa acaagttctc
    21901 aaatagtttc tgttaataga gtatatatcc ttaccatcta aaagtatact tccttcatct
    21961 atatgataaa aatttgtaat tagatttata atagttgttt ttccacttcc cgtctctcct
    22021 atgatggcat ttgatgtgcc agctttgatt tttaggttta tattatctaa aattttcttt
    22081 tttgaatcat aagaaaaact tacatcttta agctcaattt ctccctttac aaaatgagag
    22141 tttaaattac catctttatt atcgttaatc tcacttttaa acatgttatg cacttcttgc
    22201 tcttcttgtt catctataat ttcaaaaact ctttcacatc cagctatggc tgaaagtaat
    22261 gtattaaata gatttgctaa ttcatttaaa ggtcttgtaa attgtcttac ataacttaaa
    22321 aaactagcta taacccctat ggttataata tctttagatg caagaatgcc tcctataata
    22381 catatagttg caaatcctat attactaatc acattcataa taggcattaa aagagaagtg
    22441 taaatttgcg atttaaggga aatatttaga attctatcat tgacttcttt aaaatcattt
    22501 acagattttc tttcataatt aaaagatttt actacattta tgtttgatat catttcttca
    22561 atatgtgcat ttagttttcc caactcaact tgttgttgct taaaaaaaac tctagttctc
    22621 tttgcaataa aattactcac aaacattact atcggtatta atattacact gcaaaaggtt
    22681 aaaattgggc ttaatacaaa catcataata aaagtcatca atatgtttaa gattcctgta
    22741 attaactgta ctaatgaatt tgatattcct gtactgatat tatcaacatc atttgtcact
    22801 ctactcataa tatctccagt agaactttta tcaaaaaagg ttattggtag atttttaaat
    22861 tttaaaaata aattacttct catttcttta actatacttt gagataatct agctgtacat
    22921 atccccttag acagtttaac tgaaaactca attatataaa atgaaagtaa aataattatt
    22981 ccttttaaga aaatggattt tgaataagta tcgatatcta ttatgtcaac tacctttcca
    23041 atagtatatg gtattacagt tgatataatt gaatcaatta ttactagcaa aaaaataaat
    23101 ctagtaatct tcttatcttt tttaaagtac ataaaaattc ttttaaacgt ttttttaaaa
    23161 tctttaggct tttctttttt ctttaatcta ctgaccttct gatttccagt acttaaattc
    23221 aatatacttc ctccttaatt tacatcttca cttactaaat tctgcaacgc aaaaattttt
    23281 ttataaattg gagaactaac cagtaaatct tcatgtgtag caaatccaac cacctcaccc
    23341 tcatccaaga ctaaaatctt atcacagtca attatagaac taatcttttg tgaaatagtt
    23401 attacagtac atttcttact ttccttttct cttaaattac ttatatactc ttttatattc
    23461 tttcttataa ttgtctctgt attaatatcc attgcactaa aacaatcatc aagaattaat
    23521 atttgtggtt ttttggctat agctctagct atagtaattc tttgtctctg tcctcctgat
    23581 aaatttttac ccctttggaa taaaacttca tcataagtat tttcgagttt tgttataaat
    23641 tgatttgcat ttgctatttc acatgatttt acaacatcct ctttgcatat atctctattt
    23701 cccataacta cattctccaa tatacttttt ccaaacacac tactattttg aaatactaca
    23761 cctattcttg cccttaaact ttcttcagaa atactgtata tactacgtcc tccaatctta
    23821 atatcacctt tatcaattat atataatttt tgaattaaat ttactaaaga agtctttcct
    23881 gaaccagttt gaccaatcac accaatattt tgaccttctt ctattttaaa ggatacgttt
    23941 tttaatattt tttcatctgt atttttgtta tacgaaaaat ctacattttc aaaaattata
    24001 tcacagttta caaactttaa atctttttct ccaacatcta aactttcttc tctctcaatt
    24061 acttcttcat tctcatcaac caaaacctca cttattctct cataagatgc ttttgcccta
    24121 acaaaaacat taaaaacatg tgaaatcatt ataagagatg ttaatattct agtcatatag
    24181 tttacataag caactataat cccaatttta atttctcctg aataaattaa atgccttcca
    24241 attagtaata ttattattat ggaaacatta atgattattg taattgtcgg tctaaaaaat
    24301 gacatcatag ttgtagcttt tctactattt ttaaaaagtt catcatttaa ctcttcaaag
    24361 ctttggttct cataatcgta tctacaaaat agtttaacta cttttattcc acttaaaaac
    24421 tgtcttaatt ttgaatttac tttatctgta ctttcttgta catttttaaa taatctatac
    24481 cctatcttca tattaagcaa tattataata aatataatta aaactgcaat tagaataatt
    24541 attgacatct ttaaatttaa ttttatagct ataattaaac ttcctataca cattattggt
    24601 gactttataa aaactctcat cattccatta acgaattgtt gcatttgatt gatatcatta
    24661 gtaattcttg ttattaatga agctctacca aatttatcta tttgttttaa tgagtatgta
    24721 tttatctttt caaatacatc ctgtcttaaa tcataagaaa aatgctgaga aactcttgcc
    24781 gagattacac ttcttattat tgcaaatatt gcacctaaaa aagttatacc tatcattata
    24841 gctcctgttg atagtatgta gtctaagttc ttatttgaaa cccctatatc tattattttt
    24901 gccattatag ttggttgagc taaatcacaa aaagcttcca atgttaaaaa aaatactgct
    24961 gttagaaata atactatata tttttttatg tattttttaa aatacctcat tcaatcatcc
    25021 cttcatagaa ttttctcatt accaaaagta tatca
//
LOCUS       DAYAOY010000056        24959 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYAOY010000056 DAYAOY010000000
VERSION     DAYAOY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.056"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            302..475
                     /locus_tag="ACGI6B_002362"
     CDS             302..475
                     /locus_tag="ACGI6B_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751803.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            475..1086
                     /locus_tag="ACGI6B_002363"
     CDS             475..1086
                     /locus_tag="ACGI6B_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFG9751804.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEDFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLTNSLARKVVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            1163..1351
                     /locus_tag="ACGI6B_002364"
     CDS             1163..1351
                     /locus_tag="ACGI6B_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751805.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(1331..1648)
                     /locus_tag="ACGI6B_002365"
     CDS             complement(1331..1648)
                     /locus_tag="ACGI6B_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751806.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            1772..1999
                     /locus_tag="ACGI6B_002366"
     CDS             1772..1999
                     /locus_tag="ACGI6B_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751807.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            2069..2761
                     /gene="terS"
                     /locus_tag="ACGI6B_002367"
     CDS             2069..2761
                     /gene="terS"
                     /locus_tag="ACGI6B_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFG9751808.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSKE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETQDLINEIKNKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDTDKEIT
                     VKVLKASGENGK"
     gene            2751..4094
                     /locus_tag="ACGI6B_002368"
     CDS             2751..4094
                     /locus_tag="ACGI6B_002368"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9751809.1"
                     /translation="MESKFISNDHFYDFIFNWDYKFYFLVGGYGSSKSYHVATKLIIK
                     LLEEKRKALVVREVYDTMRDSCFSLLEEVAESMDLGDILSFKTSPMRVIFPNGSKIIF
                     KGMDNPAKLKSINGVSIIWLEECSEVKYTGFKELLGRLRHPSLSNHIICSTNPIGEDN
                     WTYKHFFRDEEKDRFVLDDTVLYKNRIVTKNNTYYHHSVADDNFFLPGSYIEQLDEMK
                     EYDYDLYRIARKGRFGVNGTKVLPQFEVMEHQEVINKISNIPQRYYKVGFDFGFEKSY
                     NALLRLAIDHENKYLYIYWEYYKNQTTDDVTVKDIKEFKDTQELIKADAAEPKTIKYF
                     RQQGFNISGAKKYAGSRLQNTKKVKRFKKIYCSSECKHTIKELKNLTYATDKNGNLIY
                     DEFNIDPHTFSAIWYGLDGYEVSDIKELKYSNDIYNKGLGLKKNNQYNKKGGTVF"
     gene            4094..5617
                     /locus_tag="ACGI6B_002369"
     CDS             4094..5617
                     /locus_tag="ACGI6B_002369"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9751810.1"
                     /translation="MNIKNILLNLDEKELRDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIVDNKVKPLINKQARFMFGKEPTIILKAYEKEHKEACEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSINDFKYQVDSN
                     DITKLKSVVFVRFDSSTIKEVTAKQIWYRYTYYMKKSDVSNQESCFIKIEKFKGDNLS
                     KPIKITENDTKLSKIPCWVIVNEQSITNIRGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKAKQAQAYRVESSFSNADPVN
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDNIDEELNSKSNNSDE"
     gene            5630..7249
                     /locus_tag="ACGI6B_002370"
     CDS             5630..7249
                     /locus_tag="ACGI6B_002370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751811.1"
                     /translation="MKDNTYTKKVLEARKKLLLLDKKVQLEILSVYKDASKTILSDIV
                     KNKELNLSTKYLKKLNKSIEKYINELNQRLVPVTEKSIIEASNIAKELQMYYYQSIVP
                     NESIKFACDAMCIKTTTSVVEKLVASNFYKDKRSLDSRIWGYSNKNRKDIDRLIKANV
                     ARGANAKTLAKSLDNYVNPVKKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPMCIGLQWNLSSQHYIRQVKWRGEDECDEYAEQNRYGLGEGVFPPEKYPIPHP
                     NCLCYPTQVIVPINEASKMMNDWVTGGDNDILDSWFDGIIHQNNSIPVIKKQINKKNK
                     ITTNSKDDRIKKNLTNSIKNKSKKAPKQIQKYINKYVNTNKIIIDNGQKMPFIYYTKV
                     DLIGINPNIREFKSYNKEAALLHEFAHRIDIKEIKSYNNIKFQQAVESSSMYVLKNIE
                     KLQSIYGNSNDLYNNEFISDILGALSNNEFDDLLATHSENYWSKNRNKEKEIFANLFT
                     LKYQNNKEINSFIKEHLNSLDKIFNELLGGI"
     gene            7251..7403
                     /locus_tag="ACGI6B_002371"
     CDS             7251..7403
                     /locus_tag="ACGI6B_002371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751812.1"
                     /translation="MLEEMRKDKKLLELRRLYKEKYGKNAPGFNYDEYNSYAEYKEKL
                     KELIQK"
     gene            7470..8123
                     /locus_tag="ACGI6B_002372"
     CDS             7470..8123
                     /locus_tag="ACGI6B_002372"
                     /inference="COORDINATES: protein motif:HMM:NF018515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9751813.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNIKSPRFVEKTE
                     LENANKEIKEYKKQIGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIRENAEKEIE
                     VLKFNTAFERVIESYNPRNSKALAALINKDNISFVDGKFIGLDEQIKAYQQSDSYLFN
                     NEKNKGDDEIGGTGGLEGGTTSLLDNDSEISSIGELLANSKVKSENIEAQKKFFGEE"
     gene            8135..8491
                     /locus_tag="ACGI6B_002373"
     CDS             8135..8491
                     /locus_tag="ACGI6B_002373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751814.1"
                     /translation="MSIEKSEIYMGENKTILKFAGNLFQNVNIKVKKNDVAIVDEKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRDIDFTYSNGNESIPVFIFGFIDEKTLPT
                     AIPEEAKQAMKMIMFL"
     gene            8515..9543
                     /locus_tag="ACGI6B_002374"
     CDS             8515..9543
                     /locus_tag="ACGI6B_002374"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFG9751815.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGESLFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASKAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGNPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVA"
     gene            9560..9760
                     /locus_tag="ACGI6B_002375"
     CDS             9560..9760
                     /locus_tag="ACGI6B_002375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751816.1"
                     /translation="MAKKKENLMQVKALVYLKYDNECYKIDDIFEIRKADQELMEEKG
                     YIEVIGEVEEKNNNELLNKDGE"
     gene            9763..10080
                     /locus_tag="ACGI6B_002376"
     CDS             9763..10080
                     /locus_tag="ACGI6B_002376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751817.1"
                     /translation="MSIADLDKLKLNLQEEEYPYFTDEQLEMLLESNENNVLKASWRG
                     CLLKGATDDEIKIGPIETKSSNSSYWLTLADIYKTDYLEEKSKNETTITGYKTSMTRV
                     DGQ"
     gene            10077..10460
                     /locus_tag="ACGI6B_002377"
     CDS             10077..10460
                     /locus_tag="ACGI6B_002377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751818.1"
                     /translation="MRKLRADKIIKTINRGIALNPQTITIEQEVKNIVDGAIDITNEV
                     KKLTVVIYPEKTNDTVINSETIGTAYKNKNFGMVADKEADLRLNTENEITFKCIEGTM
                     KLNYVNPIVVEGKICGYICGLEKLD"
     gene            10473..10883
                     /locus_tag="ACGI6B_002378"
     CDS             10473..10883
                     /locus_tag="ACGI6B_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751819.1"
                     /translation="MSVFTKAINEIDRKKATMPILCMNIAFMLEGEAKNNAKWTDRTG
                     NARQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHVIRPRNAKALFWNGASHPVK
                     QVKHPGTKGTHSLESTVSKNITKIGKLIEGHWSK"
     gene            10885..11709
                     /locus_tag="ACGI6B_002379"
     CDS             10885..11709
                     /locus_tag="ACGI6B_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751820.1"
                     /translation="MRAGIRKALIENIPRIKDCYEPTVPNKKTIKPYIVVVQGEDADN
                     EGDVIGFRRTINIWLYEKRTTFNKLDELTKEVVEALDFKTITDDTSNEVFTCIYDGAV
                     GQDVIDEEWEAIIRCLRFSVIALEDKEDTTNDRWVEALSKHTKDLLEIESYKDNWKKN
                     FIAPCVLWRTTNVENKRINYHLIEITKTMKCHVVSKNKDEIVKLLEALETSLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLR"
     gene            11722..11925
                     /locus_tag="ACGI6B_002380"
     CDS             11722..11925
                     /locus_tag="ACGI6B_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751821.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIENSEALGYKKEVVAGALFNC
                     KKEELTKSEFEKAIKEFLEREVK"
     gene            11927..13348
                     /locus_tag="ACGI6B_002381"
     CDS             11927..13348
                     /locus_tag="ACGI6B_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9751822.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAFKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTARNFNVTIKSNLVDSDKKDFIFFENTKQLFSSSIK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTALANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVADEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KGFNDENIVNIGSSAYYENIKYTPSEVAVYIAALSASKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVTIVDDVNTFKKYVDDKNEAIGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLG"
     gene            13364..13777
                     /locus_tag="ACGI6B_002382"
     CDS             13364..13777
                     /locus_tag="ACGI6B_002382"
                     /inference="COORDINATES: protein motif:HMM:NF020949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9751823.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSKFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            13861..14355
                     /locus_tag="ACGI6B_002383"
     CDS             13861..14355
                     /locus_tag="ACGI6B_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751824.1"
                     /translation="MSEIYKRELENEVVEEDNDLDEEIEETNEDRLKMKEDEIIAKLL
                     EDSPVPERTVFLDRLGIPITLKALTEKEISRIRKDCTKIVKVQGRREEKLNDDEFTLA
                     LIEKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDD
                     DIKN"
     gene            14361..14513
                     /locus_tag="ACGI6B_002384"
     CDS             14361..14513
                     /locus_tag="ACGI6B_002384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751825.1"
                     /translation="MGGRLTVLNNIFVKHHVPPDVYVKKNLMSQRLMKVFTQNEIEQE
                     NKAMKK"
     gene            14556..18797
                     /locus_tag="ACGI6B_002385"
     CDS             14556..18797
                     /locus_tag="ACGI6B_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9751826.1"
                     /translation="MYHIDVVISAKGDGETKSKLSAMEKYMKQTEKRMQTLNRIKVNP
                     AIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKAKKTVTARL
                     KATDNASSTVNKVNNKIKEVAKPIPPVIIRGQDESSSIIDKVKAKIQNLKADTIIKIK
                     SQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALGGLTGKIDSFVSGAISKFA
                     RLATTAGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKARQLGRETS
                     FTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTDGLTALGMT
                     ANDTTEFVDVMAATITNSNTSVELMGETFKYVGSMGGALGVSMKDLSLATGLMASASV
                     KGSMAGTSLRGGLVRLIKPPEEAASAIKKYGIELKKNKNGSLDLAGTIGSLREKLGGL
                     KDVEKGVAISSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIADMKLDTLS
                     GQFEILKSAIDDVRISVGQRLGPMTRGFVEDLIKKMPQIGDAIVGVVEKFVNNFDKIK
                     AGFQVLLPAIGSVIASVMVLKATFAFGGAIKSLSLLASTFGTAKLAAFGLTVGIGAIV
                     IAFAGMTVAISNNKTAMMDLQTRFGSFGEYVTTIMETVGGVIKLTLGNLLIMLGGIGK
                     GIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINGETSNATALLKKSTSKE
                     LQGVTKAFSTAFDQSKNVTEKKSSDIARALTNGLKGLDDQSLTMMRGLNDNMAIILSG
                     VTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLNFISKYSADSSNNLKQGM
                     SDAFNAFKEGTNIGGLKDGVTGMLNSLKATGPQALETLKGLGGKASEIFKGVDFNSSI
                     DAQKTKVLQNLNSLGLEGTQAIDTLRTIFSQASTALDTTNLKQGLSDTFNSFKIGFNQ
                     DGINGAISNMLGTINQIGPQMQEALGKMDGDMGQVFANVDFSTPIETQASKVLENLNN
                     LGIEGPKALETVRTIFSQASSQIQGSASQTAQQTNQQVVDALTQGNSAVQQAGQQLGT
                     DITSGVVSGVQAGIPTVQQKGNELGVATQQGVTDALNNATPQINNNNLTTGIDTAFNQ
                     ATATVQQGATNMYNGAKQSFSQLAQVGREAGSSLYNGATTSFNMLATNVRVACSSMYN
                     GARTSFTSLSSSAISAISAMCSSVVSQVSAMSSQIISYWNSVRATVSAPITASFNVKT
                     TQTTVKRTVNESSGGILGNILDRFADGGVATRTSICGENGAEMVIPLTNTRRSRAIEL
                     YEQTGKMLGLSSTPSQKVVGSSNIVNNVRQFTNVLDSNNKESQTQNLVSSNGNTINLG
                     GISINVQSSDNKEEMIQELLSQIESGIREALQDIG"
     gene            18879..19622
                     /locus_tag="ACGI6B_002386"
     CDS             18879..19622
                     /locus_tag="ACGI6B_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751827.1"
                     /translation="MWQKFKSMNGILKIIIGIIAIILLPITLVLLSIEFLVKAIGSKK
                     KAKIALGCILVFLTTLCTRNVYNVLSIEKEEHIPVKQVSYNDKDKGKSENNIQEQQQK
                     KEESKKMEEQKKNEEKEQNNEPTKFFTDVYLKNKNCVNMTSYEGIKSNLDIFGYKYEV
                     IEPNETTLRQINVYDNQSNDTIQFQFYENDTGEEVVTVMQYNKKDEPDKSVAISDGFH
                     TQPPKFQNHINNTNKEVNSIEEQINFMFN"
     gene            19691..20362
                     /locus_tag="ACGI6B_002387"
     CDS             19691..20362
                     /locus_tag="ACGI6B_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9751828.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVAMIEKWVDQAEPLRLIVT
                     GFGYNGLVTISNFSNTQTAGREEDRDIEITFRTYRELKIETLKKEAKSNTKTDLKDNR
                     PNNKTTPKIYTVKSSDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNVIKKGQKL
                     VIPSK"
     gene            20359..21321
                     /locus_tag="ACGI6B_002388"
     CDS             20359..21321
                     /locus_tag="ACGI6B_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HFG9751829.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLQDI
                     WIKKGDKIVLIDIAYESKKEETIFDGVIWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     SFKENTATQRIEYYCKQWNIPYYNLANTSVKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDSDKYGTLQKIKQDEKIKNTKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRLKMKLNIGTLEYIRRKFYNND"
     gene            21314..21676
                     /locus_tag="ACGI6B_002389"
     CDS             21314..21676
                     /locus_tag="ACGI6B_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751830.1"
                     /translation="MTDARFNGIARILKENMNKSVANSTFGMGCELAEITTNGLKVNG
                     YKDEIQDYLVLESLILKEDYFTFSDEALSGEYRHKHKIETTKELKPLAIGDKVLVAVM
                     GAEFAVIGRVVNAKPISN"
     gene            21654..22112
                     /locus_tag="ACGI6B_002390"
     CDS             21654..22112
                     /locus_tag="ACGI6B_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9751831.1"
                     /translation="MPNLFPINEKFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRHKRSAYSSVYGSEIKELIASNLSQNAKELEITRL
                     IKETLLVHPYTKEVGEFSFNWLENSRLVEYEFNVLTIDDENIVIDGNIKR"
     gene            22121..23254
                     /locus_tag="ACGI6B_002391"
     CDS             22121..23254
                     /locus_tag="ACGI6B_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9751832.1"
                     /translation="MQRELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKATGVITFTGMQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAECRVAGIIGNVSKGSVAVL
                     LGSINGIKSVTNKEDFKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPNVEEGQNRNGKAPIGAI
                     VTIATPQTLLINVKASFVFSNGFSQETVLNILKNKIDKYLDKIDIGGTVSYNAIYSLA
                     GSMMLADEGIQDFSNLTINDGTTNIILNDQVVGIGEIINEVIA"
     gene            23251..24021
                     /locus_tag="ACGI6B_002392"
     CDS             23251..24021
                     /locus_tag="ACGI6B_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9751833.1"
                     /translation="MIASKKGREMLLTLSPIYEQSLVMNSIYEAIGSEFDNLGLLNKE
                     IELQLFPQTATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQMKYIVNPNRLATIIK
                     SYTGADVYIKEDIAPYTFKVTANVDDVINYEDFKYITNKTKPSHLHWMTSFALKFTDI
                     EKFEVKMINRIFIDFRGNISNFLDGMWALNGSKNLSAYILYHEPISLNMKNKLFVKES
                     EVFTNLKVIIKKNLYYLNGIEMLNGNKLLNAELREEVL"
     gene            24022..24402
                     /locus_tag="ACGI6B_002393"
     CDS             24022..24402
                     /locus_tag="ACGI6B_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9751834.1"
                     /translation="MANAVTTDIARQKMCKARAGDITLPTIVSMVFGDGGIGNDGNII
                     APLSSDIALKNEVFRKDIENYVYPIPTTCRYSTTLLKNEAEGKNINEIGLIDSDGDLI
                     AIKTFGNKYKDSDMEMVFQIDDEF"
     gene            24414..>24959
                     /locus_tag="ACGI6B_002394"
     CDS             24414..>24959
                     /locus_tag="ACGI6B_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751835.1"
                     /translation="MPDELNFNNEIEEYLITTPAHANEFNNRQQKLLDNDKYLNNKID
                     TTKTELNTRIDTENEKQNIKIDQLIAGGSNVAYTQRVAIDDWVEDAENGFKATVTHSL
                     LTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEVKAELNVYVINGNAETHFINATV
                     DDNRVSEMTTYSSKKIEDRLIN"
BASE COUNT        10328 a         2677 c         4759 g         7195 t
ORIGIN      
        1 aaatttttat tgtcataata ctatttgctt aggtatatta taatgtgcat acttaaaatt
       61 aaatacttag caagcatttg aattaatata cataaaatat atgacataat tttttatagt
      121 gtaagttatt taaattaata taaataacag taattttatt atagaaaatg tatatattgt
      181 gaataataat aataataaaa tcttgtacaa aatgccaact gataattcct cgaaatgtat
      241 tgtgtattta acgttacgat tgtataatta acttataata gtgatatgga aggtggtact
      301 tatggctaca aaaagtattt taaaaaatgt agatgtaaga aaaaaggcat ttggaagaaa
      361 tctagtatca gctctagaaa atgctaaaaa taaacaagaa aaagaagttg tattaagtaa
      421 aaaatgttca gaagtaccaa aagataaaat aaaggatata ttcgggagat tttaatgagt
      481 ggctatttaa ttgtaaactt aagtaatatg ctgggagaac tggaggaaga agaagttaaa
      541 aaaattctct ccagtttttc ttgtcctctt aataaagatg tagaagactt tttaaaaaac
      601 aaagctattg aattttctaa acagggttta gctagtacac atttagtatt aacttcttat
      661 aaaggcaagc ctgttatagt tggatatttc actctagcta ataagtattt tacaataaaa
      721 agaaaaacat taacaaactc tttagctaga aaagtagtaa agtttggaca atataatgaa
      781 gaactaagaa gatatattat tggagcacct ttgataggac aaataggaaa aaattattca
      841 aataattata ataaattaat taaaggtgat gaacttctaa aaattgcatg tgacaagata
      901 aaagcagtgc agttagatat gggtgggaaa atagtgtacc ttgaatgtga agataaacct
      961 aaattaattg aattttataa ggataatgga tttgtagact ttggaaaaag aagccttgat
     1021 aaagacgaaa cagattcgtt agatggggac tatttagttc aaatgttgaa atatctaaaa
     1081 aaataaaagt acataaaatc taaaatgact atcttgatag atggtctttt tttatacaat
     1141 aaattaaaag gagaatgaaa ttatggagat taagaaaaac acacaagatg tagtatcaga
     1201 aagaaaaaat tccctagatt ctgaattcaa gatacctgca agtggtgtgt gttatatggc
     1261 tgagtttata aaggaatcta gggaaatcat aaaagaatta gataaacatt ttgaaagttg
     1321 tctagatgtt ttatctaagg caagactcta aatattttga atatgctgaa tcaagcatgg
     1381 tttgccaatc tgggaaatca gtatttttaa ctataaataa atcaaattca ttatcaggaa
     1441 tagctaaaaa gtcttcttct gaattgacta tgtaattacc aaatgcaagt agttcatcaa
     1501 aagaatcaaa attagtgtgt tggttcataa atttcttgct aagaattatt gtgcgaatct
     1561 tatcaaagtc aggttttaaa ttttgttcca ttctttcaat tttcttttga aattgcttta
     1621 aatttctaat gtcaatattt ttactcataa gaacacctcc tttcaataga atattagcat
     1681 aaaattgtgg tgaattctgc tattgtcgaa cgattgttga aggatattgt ataataacat
     1741 agaatttact atactataag gaggtgatta tgtgggattt gagataagtg gtaatttgaa
     1801 ttttgacagt gttattgatg atttaaaaaa agaagttgaa aataacccta ctatatttac
     1861 atcacaaaat gttggaaata agttcaaaga aaaatgtaaa atatgcgaaa aaatatctga
     1921 atttgaaata ctagaagatg gtaaagttaa atgtttagaa tgtgggactg aatttgaatt
     1981 gaatcttaaa gtagagtaaa caaaaaaaga atctcaatta tgaggttctt tttttattcc
     2041 caaaacaaac aaataaagag gtggtgatgt gcaagatgtc aaagaaaagg taaaacaaga
     2101 ttacttaaaa ggaatgaaac aaaaggaaat atcagcaaag tatgacatta gtttaaatac
     2161 tttaaagtca tggataaaaa gatacaactg gtcaaaggaa aaaaagaagg gtgcacctat
     2221 aaataaaagg ggtgcaccct tttctaataa aaattcagtt ggccatggtg ctccaaaaga
     2281 gaataagaat gctgaaaagt ttggtttctt ctcaaaatac ttacccaaag aaactcaaga
     2341 cctaattaat gagataaaga ataaagataa atttgatatt ctttgggaac agataacaat
     2401 tcaatatgca gcaataataa gagcacaaaa gataatgtat gtgaaagata aggaagaaat
     2461 gattaaagaa ttaaagaaac atgaaagtac agaaaatggt gagaagatag agtatgaatt
     2521 tcaatttgca tgggataggc aagcatcttt tcttaatgca cagagtaggg caatgagtga
     2581 acttagaagt ttaattaaac agtatgatga aatgattcat aaggattgga atttggctac
     2641 agaggagcag aaaacaagag ttgagaagtt gaaatgtgaa gttgataacc taagtaaaga
     2701 tgatacagat aaagagataa ctgtaaaagt tttgaaagcg agtggagaaa atggaaagta
     2761 aatttatatc taatgaccat ttttatgatt ttatatttaa ctgggattac aaattttatt
     2821 ttttggttgg aggatatgga agttcaaaga gttatcatgt agcaactaaa ttaataatta
     2881 agcttcttga agaaaaaaga aaagctctag ttgtgaggga agtatatgat acaatgagag
     2941 atagttgttt ttctctattg gaagaagttg cagaaagtat ggatttagga gatatattat
     3001 catttaaaac aagtcctatg agagttattt ttcctaatgg aagtaaaata atatttaaag
     3061 gtatggataa tccagctaag ttaaagtcga taaatggtgt atctataata tggttggaag
     3121 aatgttcaga ggttaaatat acaggtttta aagaactctt gggacgtttg agacatccta
     3181 gcttatctaa tcatataatt tgttcaacta acccaattgg cgaagataac tggacctata
     3241 aacatttttt tagagatgag gaaaaagaca gatttgtatt agatgatact gtgttatata
     3301 aaaatagaat agtaacaaag aataatacct attatcatca ttcagttgca gatgataatt
     3361 tttttctgcc tggaagttat atagaacagc ttgatgaaat gaaagaatat gactatgact
     3421 tatacagaat agcaagaaaa ggaagatttg gtgtaaatgg aactaaagta ttgccacagt
     3481 ttgaagttat ggagcatcaa gaagttataa ataaaatatc taacatacca caaaggtatt
     3541 ataaagttgg attcgacttt ggatttgaaa aatcatataa tgcactttta agattagcta
     3601 tagaccacga gaacaaatat ttatacattt attgggaata ttataagaac caaactacag
     3661 atgatgtgac tgttaaagat attaaagaat ttaaagacac tcaagaacta ataaaagctg
     3721 atgctgctga acctaaaact ataaaatatt ttagacaaca aggttttaat atatctggtg
     3781 ctaaaaaata tgctggaagt aggctacaaa acactaagaa ggttaagaga tttaaaaaaa
     3841 tttattgttc aagtgagtgt aagcatacaa taaaggaatt aaagaactta acatatgcta
     3901 cagataaaaa cggaaactta atatatgatg aatttaatat agaccctcat acatttagtg
     3961 ctatatggta tggacttgat ggatatgagg tatcagacat taaagagtta aaatatagta
     4021 atgatattta taacaaaggt ttaggattga agaaaaataa tcaatataat aagaaaggag
     4081 ggactgtatt ttagtgaata taaaaaatat cttactcaat ttagatgaaa aagagttacg
     4141 agatagaaag catgcagaaa gagatttctt attttatttg ggtgaatgta gaaataaaac
     4201 tatgggattg ttagatgatg attttttagg tcaaagttgg attacttttg ataacctaga
     4261 ctatacgcct tctcaaattg tagataataa ggtaaaacca cttataaata aacaagctcg
     4321 ttttatgttt ggaaaagagc caactataat acttaaagca tatgaaaaag aacataaaga
     4381 agcatgtgag gaattaaggc agtatattga ttcaatatta aatgccagca agttttggag
     4441 caacacacta aaagctttta agatagcaac tataacgaaa agagtcttat taaggttaga
     4501 agctgaacca aatcaaccta taagactctt ttatcattct ataaatgatt tcaaatatca
     4561 agttgatagt aatgatatta caaaattaaa gtcagttgta tttgttagat ttgattcatc
     4621 aactataaaa gaagtaacag caaaacaaat atggtataga tatacttact atatgaaaaa
     4681 aagtgatgtc agtaatcaag aaagttgctt tataaagata gaaaagttca aaggtgataa
     4741 cttatctaaa cctattaaaa taacagaaaa tgataccaag ctttctaaaa taccatgttg
     4801 ggtgattgtt aatgaacaaa gcattacaaa tataagagga attagcgata ttgaggactt
     4861 aaaaccttta caggacactt ataataaaag gttgtcagat tttaatgatt ctttaaaatt
     4921 tctaatgttt ggtcaaacag ttgtagtaga tgcgacagaa gaaacagtta atgcttgcaa
     4981 gattgctcct aatgctttaa tggcattaaa aacacttgaa gaaggttcag aaaaagcaaa
     5041 acaagcacaa gcgtataggg ttgagagtag tttttcaaat gcagaccctg taaactcttt
     5101 ttttaaaaga cttgaagata gtatgtatga aaaattagca atacctagac ctgaacaatt
     5161 acagaatata cctagtgcca aagctttgaa atatttatat acagagctta ttgcaagatg
     5221 ttcagaaaag tggaatgatt gggaacctgc aataagaagc atgttaagat taatagttga
     5281 agcttgtagt aaatttaatt gttatgatga ttggaatcat gactgggatg atttaatgtt
     5341 ttctattgta ttaaataaaa attatccaat tccagaagat gaagaagatt caaaaaggtt
     5401 ggcgcttgaa gaagttaata ataatgttag aagtcataga aattatataa aagaatttgg
     5461 agatgatgaa gattatgagg aagcatttaa tgaagtacta gaagataatg aaaagataca
     5521 atcagtagag caagaccagt tcagaaaaga tgcagaaata gaaattgata atattgatga
     5581 agaattaaat agtaaatcta ataatagtga tgagtaatga ggtgttgtta tgaaggacaa
     5641 tacttataca aagaaagttc tcgaagctag aaaaaaactt ttattattag ataaaaaagt
     5701 acaattagag atattaagtg tctataaaga tgctagtaaa actattttaa gtgatattgt
     5761 taaaaataaa gagttgaatc taagtactaa atacttaaaa aagctaaata aatcaatcga
     5821 gaagtatatc aatgagctaa atcaaagatt agtacctgtt acagaaaaaa gcataataga
     5881 agcttctaat atagctaaag aattacaaat gtattactat caatcaatag ttccaaacga
     5941 atctataaag tttgcatgtg atgctatgtg tataaagaca actactagtg tggtggaaaa
     6001 actagttgca agtaactttt ataaagataa aaggtcatta gatagtagga tttggggcta
     6061 tagtaataaa aatagaaaag atattgatag attaataaaa gctaatgttg caagaggtgc
     6121 taatgcaaag actttagcaa agagtttaga taattatgtt aatccagtta aaaagacaga
     6181 agctaaaacg ttagaagttg gaatgaataa aagtatatct tatcaagctc aaagacttgc
     6241 tagaacttct ataacacatg cttttgtaga aacaagtgtc caaaatgcaa taaataaccc
     6301 catgtgtatt ggtctacaat ggaacctaag ttctcaacat tatattagac aagtaaaatg
     6361 gaggggcgag gatgaatgcg atgaatatgc agaacaaaat agatatggat taggtgaagg
     6421 agttttccca ccagaaaagt atcctattcc acaccctaat tgtctttgtt atcctactca
     6481 agttatagtg ccaatcaatg aagcttctaa aatgatgaac gattgggtaa ctggtggaga
     6541 taatgatatt cttgatagtt ggtttgatgg aataatacat caaaataata gtattccagt
     6601 cattaagaaa caaataaata aaaagaataa aataactaca aatagtaaag atgatagaat
     6661 taaaaagaac cttactaatt caattaagaa taagtcaaag aaagcaccta aacagattca
     6721 aaaatatata aataagtatg taaatacaaa taaaataata attgataatg gtcaaaagat
     6781 gccttttata tattatacta aggttgattt aattggtatt aatccaaaca tcagagaatt
     6841 taaatcttat aataaagaag ctgctttact tcatgagttt gcacatagaa ttgatataaa
     6901 agaaattaag agctataata atattaagtt ccaacaagct gttgaaagta gttctatgta
     6961 tgttttaaaa aatattgaaa aactacagag tatatatggt aactctaatg atttgtataa
     7021 caatgaattt attagtgata tattgggagc attatccaat aatgaatttg atgatttatt
     7081 agcaactcat agcgaaaatt actggagtaa aaacagaaat aaagagaaag aaatatttgc
     7141 taatttattt acactaaagt atcaaaataa taaagaaata aatagtttta ttaaagaaca
     7201 tttaaatagt ttagataaaa tatttaatga attgctagga gggatttaga ttgcttgaag
     7261 aaatgagaaa agataaaaag ctgttagagt tgagacgatt atataaagag aagtatggta
     7321 agaacgcacc tgggttcaac tatgatgaat acaacagtta tgctgagtac aaagaaaagt
     7381 taaaagaatt gatacaaaaa taaagtgagc acttattaat taaatattaa tgagtgcttt
     7441 tgttatgctt aattttagga ggaaattaaa tgttagagta ttttaaaaag ttacttggag
     7501 atgaagaagg acaaaaagtt tatgaaaaat tatctaaaga taaagaaaat aagcttcttt
     7561 tagataatat taaaagtcct agattcgttg aaaagacaga gctagaaaat gcaaataaag
     7621 agattaaaga gtataaaaag caaataggag atagagacaa gcaattaaat gatttacaag
     7681 gcaaagttaa agataataaa gagttatcag atgaaattga aagtcttaaa aatgcaaata
     7741 aagaaattag agaaaatgca gaaaaagaaa ttgaggtttt aaagtttaat acagcttttg
     7801 aaagagttat tgaaagttat aatcctagaa actcaaaagc tttggctgct ttaataaata
     7861 aggataatat tagttttgta gatggtaaat ttattggatt agatgaacaa ataaaagctt
     7921 atcaacaaag cgattcttat ttattcaata atgaaaaaaa caaaggagat gatgaaattg
     7981 gaggaactgg aggattagaa ggcggaacaa cgtcattact tgataatgat agcgaaataa
     8041 gtagtatagg tgagttgtta gctaactcta aagtaaaaag tgaaaatata gaagctcaaa
     8101 agaaattctt tggagaagaa taggaggaaa atatatgagt atagaaaaat ctgagattta
     8161 catgggggaa aataaaacca tattaaaatt tgcaggaaat ttatttcaaa atgtaaatat
     8221 taaagtgaaa aaaaacgatg tagctatagt agatgaaaag agaattttaa aagcaggtac
     8281 attaatatca aaagatggaa aattggtaga tggtacaact gttacaaatg ataaggcttt
     8341 tggtttagta tatagagata tagattttac ttattcaaat gggaatgaaa gtattccagt
     8401 atttatattt ggatttatag atgaaaagac cttgccaaca gctataccag aagaagcaaa
     8461 acaagcaatg aaaatgatta tgtttttata aaataaatat taggaggaat taaaatggat
     8521 tggaaagact ttatagactc taaggagata gctaaatata taaaaaaatt accgctagaa
     8581 atgttaatag gcgaatcttt gtttccaaga aaaaagcaaa taggtatgga cctaaaatat
     8641 attaagggag cgaagaaaaa accagtagtg ttaaaacaaa gtacttttga tgttgctgta
     8701 aaaataagag cattaaaagc tcaaatagaa gttaaatcaa agagaatgcc attttttaaa
     8761 gagagtgttc ttgtaaatga ggaagataga caacagttac ttttagcttc taaagctcaa
     8821 aacaaagaac tattattaat gataatttca caaatatatg ataattattt agctcttgta
     8881 gatggtggcg atatgcaaat ggaaagaatg agaatgcaag ctttagctga tggagtaata
     8941 aatatcgtat cagaagatgg agacttagta tttgactttg aagttccaag caatcataaa
     9001 gaagtgttaa ctggaagtgc aacatgggat aatccagatg cagatattat aggagatatt
     9061 caaagatgga tgagaataat gagagatgaa gggaatccgt tacctaagag aatggtaatg
     9121 acaagtaaaa cctttgggta ttttgctaaa aataaagcta ttaaattaga tatagataaa
     9181 gatggtagag ttattttaac tgatgaaatg attaaaaatt atcttaaaaa taaagttggt
     9241 ttatctgttg ctatagtaag tggaacatat aaattagaag atgaaagtga ggaatcttat
     9301 ttccctgata acaaaataac ttttattcca gatggagatt taggaaaaac ttattatggt
     9361 acaacaccag aagaagctga caaagtatat ggttctaaat tagattgttc tgttgttaga
     9421 actggtattg ctataacaac aatgagatta attgacccag taacagttca aaccaaggta
     9481 tcacaattag gtatgccaag ctttgaacgt gctgatgaat gtttctttgc tacagtagca
     9541 taattaaggg gtgataatta tggctaaaaa gaaagagaat ttaatgcaag taaaagcttt
     9601 agtatatcta aagtatgata atgaatgtta taaaatagat gatatatttg agattagaaa
     9661 agctgaccaa gagcttatgg aagaaaaagg atatatagaa gttataggag aagttgagga
     9721 aaaaaataat aatgagcttc ttaataaaga tggtgagtag atatgtctat tgctgattta
     9781 gataaactaa aactcaattt gcaagaagaa gagtatcctt attttacaga tgagcaactt
     9841 gagatgttat tggaatctaa tgagaataat gttttaaaag cttcttggag aggttgttta
     9901 ttaaaaggtg ctacagatga tgaaataaaa ataggtccta ttgaaacgaa gtctagtaac
     9961 agctcatatt ggttgactct agcagatata tataaaactg attatttaga agaaaaatca
    10021 aagaatgaaa caacgattac aggatataaa acttctatga caagagttga tggtcaatga
    10081 gaaaattaag agctgataag ataataaaga ctattaatcg aggaatagct ttgaatcctc
    10141 aaacaataac tatagagcaa gaagtgaaaa atatagtaga tggggctatt gatattacaa
    10201 atgaagtaaa aaaactaaca gtagttatat atcccgaaaa aaccaatgat acagtaataa
    10261 atagtgaaac tattggaaca gcttataaaa ataaaaactt tggtatggtt gcagataaag
    10321 aagctgattt aagattgaac acagaaaatg agataacttt taagtgtatt gaaggaacta
    10381 tgaaactaaa ctatgtaaat cctattgtag ttgaaggcaa aatctgtggt tatatatgtg
    10441 gtctcgaaaa gttagattag aggtgattta ttatgagtgt gtttactaag gctataaatg
    10501 aaattgatag aaaaaaggct acaatgccaa ttttgtgtat gaatatagct tttatgttag
    10561 aaggagaagc taagaataat gcaaaatgga ctgataggac aggaaatgca agacaaggca
    10621 taacaggaac aagtttaggt ggaggaaatc aatacattgt taggttaggt catggcgttg
    10681 actatggaac tgttttggaa gaaggttcag cacctcatgt tataagacca agaaatgcaa
    10741 aagctttatt ttggaatgga gcttcccatc ctgttaaaca agttaagcat cctggcacta
    10801 aaggaactca ttctttagaa tctacggtca gcaagaatat tactaaaata gggaaattaa
    10861 tagaaggaca ttggagtaaa taatatgaga gcaggaataa gaaaagcctt aattgagaac
    10921 ataccaagaa ttaaagattg ttatgaacca actgtcccta ataaaaaaac tataaaacct
    10981 tatattgtag ttgtccaagg tgaagatgct gacaatgaag gtgatgttat aggttttaga
    11041 aggaccataa atatttggtt atatgagaaa aggactacat ttaataaact ggatgaactt
    11101 acaaaagaag tcgtagaagc tttagatttt aaaactataa cagatgatac ttctaatgag
    11161 gtatttactt gtatttatga tggtgcagtt ggtcaagatg ttatagacga ggaatgggaa
    11221 gctataataa ggtgtctaag atttagtgta atagctttag aagataaaga agatacaact
    11281 aatgatagat gggtagaagc tctatctaag cacacaaaag atttattaga aatagagagc
    11341 tataaagata attggaagaa aaactttata gctccatgtg tattatggcg aactacaaac
    11401 gttgaaaaca aaagaataaa ttatcatctg attgagatta caaaaactat gaaatgtcat
    11461 gttgtaagta aaaacaagga tgaaatagtt aagcttcttg aagcattaga aacaagttta
    11521 ataatagata aaagagtaag acttagagaa gataagaata tgtatttaac tcttgttagc
    11581 gtagttgagg atagggaatc agatatgttt acaactggac aattaacagc tgtgtttaaa
    11641 atgataggaa agataaaaag agaaggtcct actatggaca aaatttatgg taatggaaat
    11701 ttaagatagg aggtgtaagg gttggctgaa acaaataata aaaagattaa tgtgagtaag
    11761 caggaagaaa aatatttaaa aagtgatttt atagaaaata gcgaagcact tggctacaag
    11821 aaagaagtag ttgcaggtgc tttatttaat tgtaagaaag aagaacttac aaaatcagag
    11881 tttgagaaag caataaaaga gtttttagaa agagaggtga agtaaaatgg caactggtac
    11941 atggaatgaa aaagaaaaaa aggagatacc tggtttttac aatcgcttta aaacacaagc
    12001 agaaaagtct acaaacacag gattaaaggg tagattagca atgcctatta gggctaattg
    12061 gggagaagtt ggcaaggttg taacaataaa aaatgatttg agacaactta aaactctatt
    12121 tggtgatgat atgaattatt cagctttcaa gctaggaaaa ctagctttac taggtaatgt
    12181 aaaggaatta cttttatata ggctcgtaga tggaaatcaa aagaagggta cattaacact
    12241 aaaagatact acagaaaata gtgcaaaaga tgtaattaag ttagaaacta agtatccaac
    12301 agctagaaat tttaatgtaa caataaaatc caatttagta gattcagata aaaaggactt
    12361 tatattcttt gaaaatacta aacagttatt cagttcaagt attaaaggta ctatagatga
    12421 aatagtacta gaaataaact ctaatttaga taatgaatat gtaattgcaa ctaaagtagc
    12481 tgatagtgat acagcactag caaacttggt aaatgtagca cttgaaggtg gtaatgatgg
    12541 ttgcacatct attactaatg agtcttatct aaaagcacta gaagaatttg aaagatatag
    12601 ttttgattct tttgtacttg atggtgtggc tgatgaagca ttgcaggaaa ctacaaaagc
    12661 ttgggtagct aaaaataaag aattaggaaa agatatacta ctttttctag gtggaaaaac
    12721 agaggataat ataaaacaaa ttaatgataa atcaaaaggt ttcaatgatg aaaatatagt
    12781 taacattgga agttcagctt attatgaaaa tataaaatat acacctagtg aagtagctgt
    12841 ttacattgct gctctttctg caagtaaagg tataacagga agtatatgta atgctaagac
    12901 tatatttgaa gaagtagaac caagattaag ccaaagtgaa gtaaaagagt gtttgaaatc
    12961 aggtacacta atacttgatt ttgatgatgg agacgtgact atagtagatg atgtaaacac
    13021 atttaaaaag tatgtagatg ataaaaacga agcaatagga tatatctcta atatcatgtt
    13081 tattaatact ataaataaag atacttcatt aaaaagaaaa gagtttgtag gtaagatatt
    13141 caacgattcg acaggtcaaa caactgttat atgtgcattg aagaaatatt ttgaagaatt
    13201 gatgagtcaa ggtattatat cagaatttaa tgttgatata gatacagagc ttcaagcaac
    13261 tgccaaagca gatgaatttt actggaagtg ggatgctgtt aaggttgatg tcatgaaaaa
    13321 aatatatggt actggatact taggataaag gaggttatag attatgtata atgatgatta
    13381 tatagaagaa gccagttttc tgaatggttc agatgtagtt atacttattg atggtgtaga
    13441 agagctatac atggaagaaa taaaagctga ttttgagcaa gatgagcaaa gtattaaatt
    13501 gttagggtgt caaaatgaaa tatcaagggt tggtactact aaaggttcat tctccttgaa
    13561 tggatataag acagattcaa aatttgcaaa attaggattt aggtcttttg aaataatata
    13621 taatttatct aattctgaaa cattaggata tgaaagtatt agattaaaga attgtagatt
    13681 aaaaaaattg cctcttataa attctaaagc tggtgaaatt gtaaaaatag aagtagaggg
    13741 aagttttaga ggatatgatt tattaaatga actttaaaat aaaataatgt tgtactttaa
    13801 agctatgaac aattaatttt gtttgtagct ttttaaaatt aaaaatattg gaggaatagt
    13861 atgtcagaga tatataaaag agagttagaa aatgaagtag tagaagaaga taatgattta
    13921 gatgaagaaa tagaagaaac aaatgaagat agattgaaga tgaaagagga tgaaataata
    13981 gcaaagctat tggaggattc accagtacct gagagaactg tgtttttaga tagattagga
    14041 ataccaatca cattaaaagc attaacagaa aaagagatta gtaggataag aaaagattgt
    14101 actaaaattg taaaggtcca agggcgaaga gaagaaaaat taaatgatga tgagtttact
    14161 ctagcattaa tagaaaaagg aactgtaaaa cctaattttt ctaatcaaaa attacttgat
    14221 gctatgaaag taacaaatgc tagagaattt ataaagagaa aattcttagc aggagaatta
    14281 agcaaaataa gtgaccaaat attagaatta tctggttttt atgatgaaat aagtgatgat
    14341 gatataaaaa actaataaaa atggggggac gactgactgt tttaaataac atctttgtta
    14401 agcatcatgt tcccccagat gtttatgtta agaaaaattt aatgtctcaa cgcttaatga
    14461 aagtatttac tcaaaatgaa atagagcaag agaataaagc tatgaaaaaa taaatatcta
    14521 cagaaaggta ggtgagggaa atagctaaaa aggaaatgta tcatattgat gttgtcataa
    14581 gtgcaaaagg cgacggagaa acaaaaagca aactaagtgc tatggaaaaa tacatgaagc
    14641 agacagaaaa aaggatgcag acacttaata gaataaaagt taatcctgct ataaaagcta
    14701 cagataaagc ttcaagtgtt gtaaatagag ttaataacaa tatgaataaa gcaaaaaaaa
    14761 ctgttacagc aagaataaag gctacagata acgcgagtcc agtagccaat agagctagca
    14821 ataatgttaa taaagctaaa aagacagtaa cagcaaggtt gaaagctaca gataatgcga
    14881 gttctactgt taacaaagtt aataataaga taaaagaagt tgctaagcct atacctcctg
    14941 taatcatacg aggacaagat gaatctagtt ctataataga taaagtaaaa gctaagattc
    15001 agaatctaaa agctgatact atcataaaaa taaaatcaca agctgatgaa gctataaata
    15061 ctatttctcg aactaaaaat aaattacaag aatttgtgag taagagatat gaagcagcag
    15121 ttaagattag agatgaagct agttcagcac ttggagggct tacaggaaaa atagattctt
    15181 ttgtaagtgg agctattagt aaattcgcta gactggctac taccgcagga gctttaatag
    15241 gtggaattgg tgtaggttct gctgtaaaag gatttgctac ttttgaacaa agtatgaaga
    15301 atacacaagc cgtaagtgga gcaacaggaa aagaaatgga agctttaact gcaaaagcta
    15361 gacagcttgg gagagaaact agttttacag ctaaagatgc aggagacgca atgtattata
    15421 tgggtatggc aggatggaag tccgagcaaa tgataaaagc aattcctgac gttcttaact
    15481 tggcagcagc aggaggaaca gacttagcgt taacgagtga cattgtgact gatggactaa
    15541 ctgcattagg aatgactgca aatgacacaa ctgaatttgt tgatgtgatg gcagcaacaa
    15601 taactaattc aaatacaagt gttgagttaa tgggtgaaac atttaaatac gtaggctcta
    15661 tgggcggagc attaggagta tctatgaaag atttatctct tgcgacaggt ctaatggcta
    15721 gtgcaagtgt caagggaagt atggcaggta cttcgttaag aggaggtcta gttagattaa
    15781 taaagccacc agaagaagcc gcatctgcaa ttaaaaaata tggaatagaa ttaaagaaaa
    15841 ataaaaatgg aagtttagac ttggcaggaa caataggaag tcttagagag aaattaggag
    15901 gattaaaaga tgttgaaaaa ggtgttgcaa tatcctctat atttggtcgt actgcaatgg
    15961 caggttgggc ggctgtagta aatgctagtg aaagtgactt taataagtta actacagcta
    16021 ttgcagaaag tgaaggagaa gctaagagaa ttgctgatat gaagttagat accttatcag
    16081 gacaatttga aattttaaaa agtgctattg atgatgtaag aataagtgta ggtcaaagac
    16141 taggacctat gacaagagga tttgtagaag atttaattaa gaaaatgcca caaatcggtg
    16201 atgctatagt tggagtagta gaaaagtttg ttaataattt cgataaaata aaagcaggat
    16261 ttcaagtatt attgcctgca attggttccg ttatagcctc tgttatggta cttaaagcca
    16321 catttgcttt tggaggagcc attaaaagtt taagtttact tgcaagtact tttggtacgg
    16381 ctaaattagc tgccttcgga ttaacagttg gaataggggc tattgttata gcttttgcag
    16441 gaatgactgt tgctatttct aataataaaa ctgctatgat ggacttacaa acacgttttg
    16501 gttcgtttgg tgagtatgtt actacaataa tggagactgt tggtggagtc ataaagctta
    16561 cgttaggtaa cttgcttata atgttaggtg gaattggaaa aggaatagga atacttttat
    16621 ctgataagag ctgggatgaa aaagcttcat cacttaaaaa tctatttggc aaaacaacag
    16681 cagaaattaa aacaaataca aaagaagctt tatcagatat aaatggagaa acttctaatg
    16741 caacagctct attaaaaaag tctacttcaa aagagttaca aggtgttact aaagctttct
    16801 caacagcatt tgaccaatct aaaaatgtaa cagagaagaa atctagcgat atagctagag
    16861 cattaacaaa tggattaaag ggtttagatg accaatctct tactatgatg agaggtttga
    16921 atgacaatat ggcgataatt ttatctggtg tgacagcaga tatgaaacct agtgacaaag
    16981 ttaataaaat caccaaaaat cttgatgatg ctttcaaagc aggtaaatta agtgcacaag
    17041 aatacagaag tagcatacaa gagactttaa attttataag caagtatagt gcagattctt
    17101 caaataattt aaaacaaggt atgagtgatg ctttcaatgc cttcaaggaa gggacaaata
    17161 taggcggatt aaaagacgga gtaacaggaa tgttgaactc tttaaaagca actggtccac
    17221 aagctttaga aaccttaaaa ggtttaggag gaaaagcaag tgagattttc aaaggagtgg
    17281 attttaattc ttctatagat gcacaaaaaa ctaaagtatt acagaattta aatagtctag
    17341 gattggaggg aacacaagct atagacacat taagaactat tttctcacag gcttctactg
    17401 cactagatac tacaaattta aaacaaggtt taagtgatac atttaattca tttaaaatcg
    17461 gatttaatca agatgggata aatggagcaa taagcaatat gcttggtact ataaaccaaa
    17521 tagggccaca gatgcaagaa gctctaggga aaatggatgg agatatgggg caagtatttg
    17581 ctaatgtaga ttttagtact ccaatagaaa cacaagcctc taaagttctt gaaaatttga
    17641 ataatttagg gatagaagga ccaaaggctc tagaaactgt aagaactatt ttttcacaag
    17701 catcttcaca aatacaaggt tcagcttctc aaactgcaca acaaacgaat caacaagtgg
    17761 ttgatgctct aacacaagga aattcagcag tgcaacaggc gggacaacaa cttggaacag
    17821 atataacgag cggtgttgtt agtggagttc aagcaggtat accaacggtt cagcaaaaag
    17881 gaaatgaatt aggagtagca actcaacaag gtgttacaga tgctttaaat aatgctacac
    17941 ctcaaattaa taataataat cttacaactg gtatagatac agcattcaac caagctactg
    18001 caacagttca gcaaggagct acaaatatgt ataatggagc aaagcaaagc ttcagccaat
    18061 tagcccaagt tggaagagaa gcaggaagta gtttatataa tggtgcaaca acttccttta
    18121 atatgttagc aacaaacgtt agagttgcat gtagtagcat gtataacgga gctagaacat
    18181 cttttacgag tttaagtagt tcagcaataa gtgccatttc tgctatgtgt agttctgtag
    18241 taagccaagt tagtgccatg tcgagtcaaa ttatcagcta ttggaatagt gtaagagcaa
    18301 cagtttcagc accaattaca gcatctttta atgttaagac tactcaaaca acagttaaaa
    18361 gaaccgtaaa tgaaagtagt ggtggaatac tgggtaatat actagaccgt tttgctgatg
    18421 gtggagtcgc aacaagaact tctatctgcg gagagaatgg agctgaaatg gttatccctc
    18481 ttacaaacac tagaagaagt agagcaatag aattatatga acaaacaggt aaaatgttag
    18541 gattaagctc aactccttct cagaaagtag taggtagttc taatattgta aataatgtca
    18601 gacaatttac aaatgttttg gattcaaaca ataaagaatc tcaaacacag aatttggttt
    18661 caagtaacgg gaatacaata aatttaggcg gaatatctat caatgttcaa agtagtgata
    18721 acaaagaaga aatgatacaa gaactattat ctcaaataga aagtggaata agagaagcat
    18781 tacaagatat tggataatgt cgaattgttg ctaaaaaaat cttcctcata aatgatataa
    18841 tattgttaaa atatgcaaat ttatgagggg gaaattttat gtggcagaaa tttaaaagta
    18901 tgaatggaat tttaaaaatt attattggaa ttatagctat aattctttta ccaataacat
    18961 tggttttgtt atctattgag tttttagtta aagctattgg aagtaagaaa aaagctaaaa
    19021 ttgcattagg ttgcatatta gtttttttga caacgctttg tactagaaat gtttataatg
    19081 tactaagtat agagaaagaa gagcatattc ctgtgaagca agtttcttat aatgataaag
    19141 ataagggaaa atccgagaat aatattcaag aacaacaaca aaagaaagaa gaatcaaaaa
    19201 aaatggaaga acaaaaaaag aatgaagaaa aagagcaaaa caatgaacca acaaagtttt
    19261 ttactgatgt atatttaaaa aataaaaatt gtgttaatat gacatcttat gaaggtataa
    19321 agtcaaacct agatatattc ggttataaat atgaagttat agagccgaat gaaactacat
    19381 taagacagat taatgtatat gataaccaaa gtaatgatac cattcaattt caattttatg
    19441 aaaatgatac tggagaagaa gttgtaacag ttatgcaata caataaaaaa gatgaacccg
    19501 acaaatctgt tgcaataagt gatggtttcc atacacaacc accaaaattt cagaatcata
    19561 ttaataatac aaataaggaa gttaatagca tagaagaaca aataaatttt atgtttaatt
    19621 aatatttgta taatacttta aagttcttac agtttgtagg agctttttat tgtaaaaaag
    19681 aggtgataac ttggtaatag atatttatct aaaaaatgaa aaagaaaaaa tagattttca
    19741 ttttccagta aatccacaag attctttatc tataaaaaaa gaaaaaaggt ttgaaactgt
    19801 agatatagta aacttaggtg aatttgatat aaaaaaagaa ggggagaaga taagagaaat
    19861 atcatttaaa acatttctgc ctaacttata tgacgcttct tattgcagat acagtgagtt
    19921 aaaaaatcca atcgaagtag tggcaatgat tgaaaagtgg gtagaccaag ctgaaccatt
    19981 acgattaata gtaactggtt ttggctacaa tggattagtt acaatatcta attttagtaa
    20041 tactcaaaca gcaggaagag aagaagacag agatattgag ataacattta gaacttacag
    20101 agaactgaag atagagacat taaagaaaga agctaaaagt aatactaaaa cagatttaaa
    20161 agacaataga cctaataata aaaccactcc aaaaatatat actgttaagt caagtgatac
    20221 attatataag atagctaaaa atcttttagg caaaggttcg agatggccag aaatttacaa
    20281 tatacccgaa aacaaaaaag tcattggtaa aaatccgaat gtgattaaaa aaggacaaaa
    20341 gttggtgata ccttctaaat gaaaataata ttaaatggaa aatatgatat tgcaaatttt
    20401 aacgagggaa taacattaag tgaagctata gacggagttg catataagat ggatgtatca
    20461 ttagtagaac ctaaacaact tcaagatata tggattaaaa aaggtgataa aatagttcta
    20521 attgacattg catatgagag taaaaaagaa gaaacaatct ttgatggagt catatgggaa
    20581 actaggagaa gtgaaaagag taaaaaactg acattatctt gtagagaaag aactgtttac
    20641 atggaagaat cagaagaaca atattcgttt aaagaaaata cagcaacaca aagaattgaa
    20701 tactactgca aacaatggaa tataccttac tacaatctag cgaatacttc tgtaaaactt
    20761 gctaaagtaa tacataagac aaatatacta gatatgatta aaaaagactt aaaagaaaca
    20821 gcgagtaagg gtggagactt atttagagta aggatggata ataaattaaa actatttaag
    20881 cttggtacta atgcaaatgt atataaatta gatagtatat tagaagatgc aaactttaca
    20941 agtagtttta atgatgcagt aacaagtgta aaagttttag gaaagagtaa agatgaaaat
    21001 acaaaagcac ctatcattgg aacatataaa aaggactcag acaaatatgg aacactgcaa
    21061 aagattaaac aagatgaaaa gataaaaaat actaaagaag ctaagaaagc agcagaagca
    21121 atgttcaata gtggagagga aacaataagt gtagattgtg cagtagatat aaatagaata
    21181 agagcaggtg acaaggtaag tttaaaaagt aaagaatatt atgttataga tgtcactcat
    21241 acactagatt ctagactgaa aatgaagctc aatataggga ctttagaata tataaggagg
    21301 aagttttata acaatgactg atgctagatt taatggaatt gctagaatat tgaaagaaaa
    21361 tatgaataaa agtgtagcaa atagcacttt tggaatgggt tgtgaacttg cagaaataac
    21421 aacaaatgga ttaaaagtaa atggttataa agatgaaata caagattatt tagtattaga
    21481 gagtttaata ttaaaagagg attattttac tttttcagat gaagctttaa gtggagaata
    21541 taggcataag cataaaatag aaactacaaa agaattaaaa cccttggcta taggcgataa
    21601 ggtgttagta gcagtcatgg gagctgaatt tgcagtaatt gggagggttg taaatgccaa
    21661 acctatttcc aattaatgag aaatttgaaa ctgtagaatt aaaaaataat gatgaaaatg
    21721 aattggacct aaagggttct tttttatttg attttgaaaa aggtgaattt gttaaaaatg
    21781 cagatggaac attaaaaaaa tgcgataaag ttcaagcgta caaacaatgg tgtcagaaag
    21841 ctatattaac acctaggcac aaaaggtcag cttattctag tgtatatgga agtgaaataa
    21901 aagaattaat cgctagtaac ttatcacaaa atgcaaaaga gcttgaaata actagattaa
    21961 taaaagaaac tcttttagtg catccttaca caaaagaagt aggagagttt agttttaatt
    22021 ggctggagaa tagcaggtta gtagagtatg aatttaatgt attaacaata gatgatgaaa
    22081 atatagtaat tgatggcaat ataaaaaggt aggtgattat atgcaaagag agctacctat
    22141 accagtattt ttaacagaag atgaggactc tgtacatgaa aggatgttaa gtaactttca
    22201 agatgtgagc acactagaag gggactttat ttatgatgca acaagaccta cagcagagca
    22261 gatagccgaa ttaaaacaac taggattaca aaataattta aagatagcat ttcctcagac
    22321 ctcttatgga acttatttag agtggcttgg tgaatgtaaa ggagtattta aaaatcaacc
    22381 aactaaggct actggagtta ttacatttac aggtatgcaa ggaactatca ttacaaaagg
    22441 aactatagta actactattg caactgatga aaaacagagc atagaatttg agcttcttga
    22501 aactaaaact ataggagaaa atgaaacagt agatattaaa gcagaatgta gagttgcagg
    22561 aattataggg aatgtgtcta aaggtagtgt agccgttttg ctaggttcta ttaatggtat
    22621 taaatcagtt actaataaag aagatttcaa aggtggaaca gatattgaag acgaagaaca
    22681 ttttagagaa agagttcttg tagcagaaca agaagataaa cttagtggag ctagttcaga
    22741 ttatataaga tgggctaaag aagtagatgg agtgggatat gcttatgtag ttcccgaatg
    22801 gaatggagca gggacagtaa aagtattaat actagataaa aataggaaag cagcaacaca
    22861 agaattaata gataaggtcc aagaatatat atacccaaat gtggaagagg gtcaaaatcg
    22921 aaacggaaaa gctcctatag gtgctattgt aactatagct actccacaaa cattactaat
    22981 taatgtaaaa gctagttttg tgttttcaaa tggatttagt caagaaactg tgctaaatat
    23041 cttaaaaaat aaaatagaca aatatctcga taaaatagat attggaggta ctgtttctta
    23101 taatgcaatt tattcactag caggttctat gatgcttgca gatgaaggaa tacaagattt
    23161 ttctaactta acaataaatg atggaacaac aaatattatt ttaaatgacc aagttgttgg
    23221 aataggtgag ataatcaatg aggtgatagc gtgatagctt ctaaaaaggg tagagaaatg
    23281 cttctaacat tatctcctat ttatgaacaa tctttagtga tgaactctat atatgaagct
    23341 ataggaagtg aatttgataa tctaggatta ttaaataaag aaatagagtt acaattattt
    23401 cctcaaactg ctacatgggg acttgaattt tgggaaaata gggtaggttt atctactaat
    23461 atagatgaag atatagaagc tagaagaaga aaagtcattg ctaagcttca aatgaaatat
    23521 attgttaacc ctaacagact agcaactata ataaaaagct atactggtgc agacgtatat
    23581 ataaaagaag atatagctcc atacactttt aaagtaactg ctaatgtaga tgatgttatt
    23641 aattatgaag atttcaaata tataacaaat aaaactaaac caagtcatct ccattggatg
    23701 acttcttttg cactcaaatt tacagatata gaaaaatttg aagtaaagat gattaatcga
    23761 atatttatag attttagagg gaatataagt aatttcttag atggtatgtg ggcattaaat
    23821 ggcagtaaaa acttaagtgc ttatatactt tatcatgagc caataagctt aaacatgaaa
    23881 aataagttat ttgtaaaaga aagtgaagta tttacaaatc ttaaagtaat cattaaaaag
    23941 aatttgtatt atttgaatgg aatagaaatg ttaaatggaa ataaactgct taatgcagaa
    24001 ttaagagagg aagtgttata agtggcaaat gcagtaacaa cagacattgc aaggcaaaaa
    24061 atgtgtaaag ctagagcagg tgatataaca ttgcctacta tagttagtat ggtgtttgga
    24121 gatggaggga ttggaaatga tggaaatata atagctccac tttcaagtga tatagcgtta
    24181 aaaaatgagg tgtttaggaa agatatagaa aattatgtat atccaatccc aactacttgc
    24241 agatattcaa ccactttatt aaaaaatgaa gcagaaggaa aaaacataaa cgaaataggt
    24301 ttaatagatt ctgatggtga cttaatagca ataaaaactt ttggaaataa gtataaagac
    24361 agtgatatgg aaatggtttt tcagatagat gatgagttct aggaggtgaa taaatgcctg
    24421 atgaattaaa ttttaataat gaaatagaag agtatttaat aactacacca gctcatgcga
    24481 atgagtttaa taatcgacaa caaaaattgt tagacaatga taaatattta aataataaaa
    24541 ttgatacaac taaaacagag ttaaatacta gaattgacac agaaaatgag aaacaaaata
    24601 ttaaaattga ccaattaatt gcaggtggtt caaatgttgc atacactcaa agggtcgcaa
    24661 ttgatgattg ggtggaggat gcagaaaatg gattcaaagc aactgtaaca catagtttgt
    24721 taacacagag aatagttgta aatattatag atgctactac aaaagaaaat gtagttacaa
    24781 actttaaaat tatagatgat aattctatag aaattagaag tgaagtaaaa gctgagttaa
    24841 acgtttatgt gataaatgga aatgcagaaa ctcattttat taatgcgact gtggatgata
    24901 acagagtgtc tgaaatgact acttattcgt ctaagaaaat cgaggataga ttgattaat
//
LOCUS       DAYAOY010000057        24551 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYAOY010000057 DAYAOY010000000
VERSION     DAYAOY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.057"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(441..2828)
                     /locus_tag="ACGI6B_002395"
     CDS             complement(441..2828)
                     /locus_tag="ACGI6B_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9751836.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="ACGI6B_002396"
     CDS             complement(2851..3216)
                     /locus_tag="ACGI6B_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9751837.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="ACGI6B_002397"
     CDS             3415..4263
                     /locus_tag="ACGI6B_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751838.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="ACGI6B_002398"
     CDS             complement(4700..5788)
                     /locus_tag="ACGI6B_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751839.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="ACGI6B_002399"
     CDS             5913..6641
                     /locus_tag="ACGI6B_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9751840.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6845..7336)
                     /locus_tag="ACGI6B_002400"
     CDS             complement(6845..7336)
                     /locus_tag="ACGI6B_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751841.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7347..7790)
                     /locus_tag="ACGI6B_002401"
     CDS             complement(7347..7790)
                     /locus_tag="ACGI6B_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751842.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7780..8619)
                     /locus_tag="ACGI6B_002402"
     CDS             complement(7780..8619)
                     /locus_tag="ACGI6B_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HFG9751843.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8838..10223)
                     /locus_tag="ACGI6B_002403"
     CDS             complement(8838..10223)
                     /locus_tag="ACGI6B_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9751844.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10396..11139)
                     /locus_tag="ACGI6B_002404"
     CDS             complement(10396..11139)
                     /locus_tag="ACGI6B_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFG9751845.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11159..12421)
                     /locus_tag="ACGI6B_002405"
     CDS             complement(11159..12421)
                     /locus_tag="ACGI6B_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9751846.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12605..13603)
                     /locus_tag="ACGI6B_002406"
     CDS             complement(12605..13603)
                     /locus_tag="ACGI6B_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751847.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13587..15098)
                     /locus_tag="ACGI6B_002407"
     CDS             complement(13587..15098)
                     /locus_tag="ACGI6B_002407"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFG9751848.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15152..16114)
                     /locus_tag="ACGI6B_002408"
     CDS             complement(15152..16114)
                     /locus_tag="ACGI6B_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9751849.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16162..17052)
                     /gene="rbsK"
                     /locus_tag="ACGI6B_002409"
     CDS             complement(16162..17052)
                     /gene="rbsK"
                     /locus_tag="ACGI6B_002409"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFG9751850.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17094..18116)
                     /locus_tag="ACGI6B_002410"
     CDS             complement(17094..18116)
                     /locus_tag="ACGI6B_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9751851.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(18336..19313)
                     /gene="bioB"
                     /locus_tag="ACGI6B_002411"
     CDS             complement(18336..19313)
                     /gene="bioB"
                     /locus_tag="ACGI6B_002411"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFG9751852.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19519..19782)
                     /locus_tag="ACGI6B_002412"
     CDS             complement(19519..19782)
                     /locus_tag="ACGI6B_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751853.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19852..20925)
                     /locus_tag="ACGI6B_002413"
     CDS             complement(19852..20925)
                     /locus_tag="ACGI6B_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9751854.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21173..21931)
                     /locus_tag="ACGI6B_002414"
     CDS             complement(21173..21931)
                     /locus_tag="ACGI6B_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9751855.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21928..22848)
                     /locus_tag="ACGI6B_002415"
     CDS             complement(21928..22848)
                     /locus_tag="ACGI6B_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9751856.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22845..23045)
                     /locus_tag="ACGI6B_002416"
     CDS             complement(22845..23045)
                     /locus_tag="ACGI6B_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFG9751857.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23050..24144)
                     /locus_tag="ACGI6B_002417"
     CDS             complement(23050..24144)
                     /locus_tag="ACGI6B_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9751858.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
BASE COUNT         7968 a         4450 c         2562 g         9571 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gagaatatat taaatttagc tttatgcttt aatgtatatt
     6781 catatatatc atttacactt atctaaacta caatttcatt aaaattaaat ataaaaatca
     6841 actattattt tttaaatagc atctttctta atctattatt gtaataacct ataaaagaac
     6901 tgtaaacata gtcatatact ataaaggcta tctcaaatga acctataata aatatgttta
     6961 ttggaatata aactattgtt cttaataaaa aatacaatat aagtatcatt atatttgcaa
     7021 atactaattt cataaaatac tcaatatata tagacctatc tttttctata agatatttta
     7081 caataccata tattccaaag gtaagtgaat atacaatcca ttgagactta cttgccatca
     7141 ctataaatcc taaaactata gaacctatat aaaaagcaat tccactctta aagccccatt
     7201 ccattattac aattgatact aaaagtgatg ctaaccccat aataaacaat gtatttacag
     7261 gaataatatt tgtaagagtc aacaatatca catttaatgc taataaaact cctccataag
     7321 caacttttat actcatattt atcatcctaa caacaagtta ttaaatctcc accaaaacat
     7381 tcacaacatg aatctaaaca ccaaagctta gcacaatttt cacaacaatc gtcacctaga
     7441 caacaacagc aatcatcaca gcaacagcaa caacatccat ctaaatcatt acgtcttctt
     7501 ctattataat tgtcagccct attgctataa tcatttctat attgtgtata acttctaagt
     7561 gcatcactat attcactatt ttcaggctcc ataaattttg ctctttttaa gaagtcttca
     7621 ccttcttcat aatatcctat gttcattgca gaaaagccag ttaaataata ccactctgca
     7681 catttgtcag tcaaagtttt taaaaaatca tatgcttttt taaattcact attttctatg
     7741 aatgctcttg ctctcataaa attttcatta ttataagtat tactgtacat tctttccacc
     7801 tctactttct aatatattaa tatatcttaa ataaacacca gaataaatta tattttctat
     7861 tattccaaca tttttattta gatttagatt atcaattccc ctagctaaaa accctaatga
     7921 tgttactatt atttttttta ctttttcttt taactcatca tttttatcaa tatactctat
     7981 aaatggatta tatcttcctt tcttaaaatc ttcatctaaa tcttcatagg catctagtag
     8041 atatatatac ttacctatat taaaacctat cattcttaag tttctctcat tttcatcttt
     8101 tttatagaca aatacttcac ccattatatt tccaaatata ctagatactt catctatatt
     8161 tatattttta tctttttcta acttatttaa ttcatttagt tgttgtttta tatactctgc
     8221 tttttcagga tacttttcat atgctaattt taatttgttt ttatatatat aatatgccaa
     8281 tttatctttt atgcgtttat catccattaa attatcttcc aatttataat acgttaaaag
     8341 aatattcata cttgctgcat attctgtaat atcatttgta attacctttt tctttttaaa
     8401 tggatttact atacacactt cctctgtact tatagactct gggttatata ctgctgttaa
     8461 taaaacaatt aaaaatgtta tatcataatt caaagaaaac ctagagattt ctccatgatt
     8521 tctttttaaa tatttacata gtccacaata gtaagctttg taataatcgt attctctaaa
     8581 tgttaaatcc attttgttta ttttaacata tccaaacata aatactcctt tttgtactat
     8641 tttttattta gattatatat agtataacac tttttatcaa gattatataa tttacagtat
     8701 tctaagaaac ttaaaaaatt ccttctaatc attgtatctt aaatttaaaa taattagaag
     8761 gttgggttaa aaataatatt aaattatata cttatattaa ctcatacaag ttagttgtgc
     8821 ttatatcaat ttaagtttta aataattggt atatatttaa aactacctac agtatccttc
     8881 aattcttttt ttgctttatt tataaccaat tcatcaagca ttacatcatt tattgaacat
     8941 gatatagatt tagatgccaa taacattgct ttaaatccca tatcagaacc tgcactggca
     9001 caagattgcc atgtatgggc agaaatacca ataggccaaa ctgaacaaga acactgtgct
     9061 gtaggaatta tgtaactaac atctccaata tccaatgata atgatataca gttattatga
     9121 gtaaatgtat catccgtaac ttcatcatgc agattcatct tagtaatatc cttatttcca
     9181 cttaatacac tagaaacact acttctttta tctatagaca ctgtctcagc taatttatct
     9241 gcaaaatagt agtcatcttc attatagttt tgtacaccta ctaatttcat attttgtgat
     9301 agaatatctg ttaatattgt atttggaata tagtcatata ctccacttat aatgtcatat
     9361 tccataatag taccagtcat taatgctgct ccttttgcta tatcaacaat cctaccaaat
     9421 acatctctta catgctccat tttaactcct cttatgtaat accacacttc tgcaaaatca
     9481 ggaactatat ttggcatttt tccaccatta gtagttacat agtgtactct acaattatca
     9541 aatatatgtt ctctcaaata attacaacct atattcataa gttctactgc atcaagtgca
     9601 cttcttccgt tataaggagc ttgtgctgca tgggcactaa tcccattata tctaaatttc
     9661 acagagtaat tagcaagtgt tgggattctt ataggagtat ttatatcaaa tggatgccaa
     9721 ctaaaagcac aatcaattcc atcaaaaaaa ccttttttta tcatcaatac ttttccagat
     9781 aaatcttcct ctgctggaca tccatagtat tttatagtcc ctttcagctt ttttagtttg
     9841 attaattcct ttactgcaat tacagctcct attgaacctg tacccagtaa gttatgtcca
     9901 catccatgac ctgcaccacc atctacaata ggttctttaa tagtggatac tttttgagac
     9961 aaactatcta aagcatcata ttctcccaaa actgctataa ctggtctacc ttctccatag
    10021 ctagccgcaa atgctgtatc cataccttct aattcatcta tactaaaacc atattttttt
    10081 aagtattctt tttgtaaatt tgaagcataa tattctttat actgaagttc tggatttttc
    10141 catatcttat ctgatatatc tcttaattct ccctgtatct catctatata ttccatcaaa
    10201 aactttttat ttaaattctc catattaatt atataaacct ccctgtttct aataacttat
    10261 cgattatcag ccaaaatgac taattttaac tgatttaaaa atattttcta tcatacaacc
    10321 attttataca ataaaacctc ttgacttgta agtttcattt tatcaagtaa gaggttttca
    10381 agttttataa aatttttagc atgttaacca atcaagcata ttaacccata atttattata
    10441 gtatttccaa tcagtaaatt cttttggacc ccagtgaggt gcacagtcag aactaaagat
    10501 agcagatttt ccttttccaa actcgccaac agctacaaat gggtctcctc caatagttgc
    10561 taaaacagga caattatctc tagctactgt tttgttatat cctaaaaact ttggccattc
    10621 tgttggtata ccttttaata ctggatgttt agagtcaata acttctggaa ttattcctgc
    10681 tggtttttca actctatcat ctttatctat cattgttatt ggtaatacat ctttgatagc
    10741 agtttctccg aatctagctt ttgcatctat tcctgtaaat gacatatatc cacctaccat
    10801 gattaacgcc ccaccattat ttacatactc tttgattagt tcaagtctat ctggattact
    10861 attgcaatct ataaaagtac tattagataa taagaacgta ttagaaccta tatcagataa
    10921 tattatacag tcatactcat ttaactcttc taatttatac ggaaaactat ctgctgctac
    10981 atgtgcaggc atatgaactg tctcatatcc tccagattct attgcttctt tcaaccactt
    11041 tacagcttct tcataatgag ttgtaacaaa cgtatcaaat ccttttatat gagttatatt
    11101 ttttacccaa gattccccta ctattaatac ttttttcatt tatatattcc tcctactttt
    11161 ataagtttaa tattagactt gcatacataa gtatactatc taaatactcc ttaatttcaa
    11221 catattcatc aattgaatga cattgttcta ttgaacctgg ccctaatata agtgttggta
    11281 tttcagctaa atttttcatt aatctcgcat cattgccagc tgttgaacct tttatctcta
    11341 attttttccc tagagtttct tctagtattt tatgtgcatt cactacaaaa tcagaatcct
    11401 tgtccatctc aaaacctaac cctgattgat atatgtttat atttggttta ttatctttaa
    11461 gccataaatc tccattagcc cttgtcatta tgacatttgt tacatcattt acaacttgtt
    11521 cataactcat tatatttggc aagaagtgta cacataaatt aaatacacac ttatctggca
    11581 cagtagaacc tgctgttcca ccatttatta cacctaaatt tattgtaggt gaaggtaata
    11641 gtggatgttt atatatcata agccagttat gttccaactc ttttatatct tgaagcaaca
    11701 acatagcctt ctcaattgca tttacaccct cccacttact accacagtga agagacacac
    11761 ctttaacctc aacttcaaag aatacaaatc ccatatgtgc aactattaag ttttgttctg
    11821 atggttcaca aattatgcaa caatctccat caataccatt cataactgca tttatggtac
    11881 catttccgcc accttcttca tctactactg acattatttt tacattacca ggtacattta
    11941 gaccactatc ttttataagc tttacagcta atattgatgc tataagtcca gatttcatat
    12001 cagcagttcc aagtccataa agttttccat tatcttctgt agcacgatat ggattatatt
    12061 tccacttact tatatcacct ggtggcatag tgtctacatg accattaaat acaattgtct
    12121 taccaggtaa atcatcacta tacttacaaa ttaaattata tctatcttga taattgtgac
    12181 ctagatttcc ttctttatat atattttttg cttttttaat taattcttcg ctcatttctt
    12241 gtctatctac actaaaacct atgtaatttg caagtttttc aatatattcc tgtccttctt
    12301 tttcaaatcc acctagtatt ccatgaccaa catcttcagt ctttatactt acaagttctt
    12361 ttaaataatc tatgtattct tgttttttac tatttaatgt atctaagaga atttttttca
    12421 tatttcctcc taagatttat ttttacattt aaaatttcaa gtctaatctc atctatttaa
    12481 gccatagtaa actttttcac ttagcatatt cccattcaat gtataaagat gtatgcaaat
    12541 taaagtataa aaatatatac aaaaacttaa taatttttat agtttataag ctaaaattta
    12601 attactattt agatttagct gattttttta attttatatt atttatccat atagcaaata
    12661 acattaccaa accttttata atcatttgat tatatgaaga tacacttaat aagtttaatc
    12721 catttcctaa tattcctatt attccgacac ctatacatgt acctactata gaaccagaac
    12781 cacccatcat attagtacca ccaagcacaa cggctgctat agcatccaat tctgctccat
    12841 ctccaacagt tggtgaacct gatgccaaac gtgatgatag tattatccca gctaatgctg
    12901 ttagtagtcc agctagtgcg tatactttta ctataacttt atttactttt actccacata
    12961 atctagcggc ctcttcattt ccacctactg catgtacatg acgtccaaaa ttagttcttt
    13021 ttaatagaaa atgtcctgct attacaacta taatcataat tataactggt attggtattg
    13081 gtccaatata tcctctaccg agcatcttaa atgcatctgt atttactgat ataggtcttc
    13141 ctcctgtaaa tacaaagctt gccccactta ttatcgttaa catggctaaa gttacaatga
    13201 atggaggtag tttttgtgtt gaaataattg caccttttat gactcctaaa attgtcccta
    13261 ttgctaatgc aagtatcata cctacccata taggtgcatt ataattcact actgttgtag
    13321 cccaaaatgt acctactaaa gcaattactg ctcctacact taaatctatt cctcctgtaa
    13381 ttattacaaa tgtcatacct actgcaagta ttgcatttat tgatatttgt cttccaacat
    13441 taattaaatt atctaaagtt aaaaagttag atgaagctac actcattatt ataaaaatta
    13501 ttacaagtcc tacagctgca ctcccttggt ctctaaatac acttgttaat tttgtcaata
    13561 atggagaaga tttttcttct ttatgcttag ttattgaact cattaatttt acctccgaaa
    13621 gccaatttta atacattatt ttcaaccatt tcttcacctt ctaaaaatcc acttatatga
    13681 ccttctctca taaccattat tctgtcactc attgatatta attctggtat ttcagaagat
    13741 atcattatta tacttacacc actctccact aaatctccca ttagattgta tatctctgcc
    13801 ttagcattta catctatacc tctggttggt tcatccataa tcaatatatc tggttgtaat
    13861 aaaagccatc ttgctattat agctttttgt tggtttccac cacttaaact ttgtataact
    13921 tgattcatat gtggtgtttt tatctttaaa gcttctatgt atttttctaa aatctctttt
    13981 tccttttttc tatctataat tgatttaaac ttagatatct tttttaaccc agccatagtt
    14041 gtattttctt tgacactcat ttgtaaaaac aatccagtac cttttctatc ttctgttatt
    14101 aatcctagtc cattttctat tgcatctgat ggagttttaa actttacttc ttcattgttt
    14161 aaatatattt ttccagaatc atatgttcta agtccaaata tagcttctac tatttcactt
    14221 ctcttacttc caactagtcc acctattccc aatatttcac cttttcttat attaaagcta
    14281 atatcattaa aaacattgtt cttagttaga ttttccactc gcataatttc ttcttttatt
    14341 tctacttttc tttttgggaa aaattggtct agctttctat ctatcattaa attaactaat
    14401 tcttcctcag acgatacatc tttggtattt aaagtcgaaa tcgttttccc atctcgcatt
    14461 atagttattc tgtcacaaat ctcaaagagt tcttccatcc tatgggatat atatattata
    14521 ctaactccac tctcttttaa atcatttata agtctaaaaa gtatttcagt ctctttttta
    14581 cttattgaag aagtaggttc atccattatt aatactttag tttctgatga taaaactctt
    14641 gctatttcta ccatttgttt ttgagctata ctaagctctt tgactaaact atttggattt
    14701 atatctattc ccatattatc gaaaatcttt ttagcttctt cattcatttt tttgtcattg
    14761 gttaagactt tgccatttat ttcttttccc ataaaaatat tttgtgctac tgttaattcg
    14821 tcaaaaactg ttagttcttg atatatggca tttataccta tctttttaga gatatctggg
    14881 gatatattct ctatttcttt gccttcaaaa ataaattttc cagaagtttt cgaatgtgcc
    14941 ccagttaaaa tctttatcat tgttgattta ccagcaccat tttcacccag tagtgcatga
    15001 acttcgccat gaaataactc aaaattaaca ttatctaatg ctctgacacc aggaaattct
    15061 ttagcaatat tacttaactt cagaattata ttactcataa tgacactcct ttttaaatag
    15121 gatttctttc aaataatctc attttatttt actaattctt aaagtctttt atattttcct
    15181 tatcataaag taaagctggt actggtatat tcttttctac cttttcccct tttatagctt
    15241 ttactactgc ttctacagct aaacgtccca tctctttagg ttgttgttga atcatagcaa
    15301 gaatagttcc atctgttatt ccacttgatg cttcttcagt taagtcaaat cctacaatct
    15361 taacatcctt attttttgca gattcaagtg cagctttagc tcctagtaca ctattttcag
    15421 ctgttgcata tattatatct acatctggat tagattgaat taagttttct actgtattaa
    15481 gagattcttc tctgtcatat cctggttgtg aatttaatat cttaacattt gctgaacctt
    15541 ttaatgcatc tttaaatcca tctattctct gcatttgtat ttgagatttt aaatctgtca
    15601 ctattgctat ttctgacttt ccacctaact tttcatctat gtatttttta gtgtattccc
    15661 ctaatttttt accagcatca taactaactg ttcctacata acttgtaata ttttcagtat
    15721 ttaatacagc atctactgta actactggca ttttttcaaa tgctccttct actgctggta
    15781 ctatagccga attatcagtt ccaagtacta tcattccatc tacattctgt tgtataaaat
    15841 cttctattgc tgatgtttgc ttagctacat cttggtctgg gtcatttact ataactttca
    15901 cacctaattc tttagctttt tcttttattc cctcatcaat atctataaaa aattgatgtt
    15961 gaagagttat tgtagatact ccaatagtta aatctttctt gtctgaacca cctttattat
    16021 ctgatttact tggtccttcc attgaacacc ctgttaacat tcctattgct agtattccag
    16081 caattcctaa agataaaatt tttctcactc tcataattaa cctccatttt taatagtttt
    16141 aaatttgaac aaattagatt tttatttatt atttaaatac ttttcaacat cttctttata
    16201 gggcatacca gattgtgcac ctagtttagt tacagaaaga cctgatacta ctactgcata
    16261 ttctactgct gtatataaat ttttcccttc tgttatagcc actgccaatg ctccattaaa
    16321 tgtatctcct gctcctgtgg tatcttgaac atcaacactt atacaagata catgcttgat
    16381 ttcctcacca tcataaaatc tagctccatt ttttccttct gttattacaa gtttatttgg
    16441 atattttttt aatacttctt ctatcccttc atttgtgtca aaaactatct tatattcatg
    16501 ttcatttggt gttaagtaag ttactttttc tattatatct tcatttagtt ttactgctgg
    16561 tgctggattt aataaaaccc taatcctatt ttcaaaacag aaattcacta cataatttat
    16621 tgtttttaat ggaatctcta attgtagcaa tactatatct gcattcttta tctcttcttc
    16681 attcttttta attaattcta tatctactag ctcattagtc cctggtacaa caacaatact
    16741 attatcattt tcacataatg ttataacagc aacaccagaa cttttgtgat tatttctgga
    16801 tactaagcta taatcaactt tattcgttct aaaatttctt attaactcct ctcctaagcc
    16861 atcttcccct atgcatgata tcatttttac atttgctcct agtttagaag ctgagatagc
    16921 ttgattagcc ccttttcctc caggtgatgt aaaaaaacta ttacctaata ctgtttctcc
    16981 tttttctggt cttttatcta cagaacatac catgtccatg tttacacttc ctatgacaac
    17041 tatatttccc attacattac tcctttacat atctatttat gtctctcgtg ctgttattca
    17101 ctttcttttg ttgaatctct tatgaccaat tctggtttga aaatcaattc atcaaacgat
    17161 tcttttttat ctcctcttaa acaatttatc aatatttcac tggctttata tcctatatca
    17221 taagacggct gttttactgt ggtaagagaa gggcttatta gacttgatgt ttgaatatca
    17281 tcaaatccca cgacacttat atcatttggt atacttaatc ctctttcttt aagacctctt
    17341 atgacaccta ttgcaatcaa atcatttgca caaaatattg catcaaaatc tatcttttct
    17401 aaatttttta taaattcttg tccccattca atcttatatt ttccttcaaa tacataagca
    17461 ggattgtagc ttaaattaca ctcgattaga gcctttttat atccttctaa tctatctact
    17521 gctatttcat tttgaagtgg accacttagg tatattattt tcttatttcc aatatctaat
    17581 aaatgttttg tagctatata ggccccttca aagttattta ttttaacaat tccttttaag
    17641 gtttctttgt tcatacttat cttcttatca atcaagacag ttggtatttt tatatcttta
    17701 taatttgctt ctttccagtt tgtatttgaa gatgctgcaa atataattcc atctatcatc
    17761 ctctctgata gtgttcttat tccttcatat tcttttttag catcttcatc tgtattacat
    17821 aaaattatat tgtaaccttc ttcattagct ttgtcttcta cacctcttga tacatctgta
    17881 aagaatggat ttcgtatatc tggtataaga agccctatag tattagtttt tttagttact
    17941 aaactttgtg ctaatttatt tggtacataa tttttttcct tcattatttt caaaattttt
    18001 tctcttgttg cctgacttat attttcatct ttattattaa tgacttttga cacagttgtt
    18061 ttagaaacac cagccaattc gccaatttct tttatagtta tactcaagaa gttcaccccc
    18121 aaaatttagg aaaccgtttt acttaaccgg ttacctagat actatcacat cttttttcca
    18181 tttgcaacca ttttatataa attttgatat ttttcccttt tttcaaaata ttgcaactta
    18241 taattcaatt ttaatattta aattaatctg caaaaaaaaa ttaatatact tttataatta
    18301 tctaaaagta tattaattaa tttaagcagt atttattaaa gttcaaatcc ttgctctata
    18361 atcatttttt tatcattttc aatcccactt ccaacagttg tcaaataatc accaacaatt
    18421 gcagaattta ctcccccatt aaatccaact ttgtcatatc cctttaatga tagccttcca
    18481 ccagcatacc ttatgtacac attaggaatt ataaacctaa atactgctgt tgtttttagt
    18541 atttccattg gttctaatac atcataattt tccattggtg tattcttaat aggatttaaa
    18601 atgtttattg gaaatgattt tacatttaat tctcgtattt caaaagccat ttttagtctc
    18661 tgttcttcac tctcattcat tccaattata ccacctacac atacatctag cccagctttt
    18721 ttagcatctt ttatagtttt tatcctatct ttatatgtgt gtgttgtaca tatattatga
    18781 aaattatctc tacacgtctc aacattgtgg tgatatgtag tcacacctgc tgacttcaac
    18841 attttagctt gctcataatc tataatacct aaagatgcac ataattttaa attagtattt
    18901 tccctcaatc cttcatagat atttaaaata ttattaaact cctttcctga cataccttta
    18961 ccacttgtaa ccaatgagaa tctatgcact cctttactct ccatctcttt agctctattt
    19021 aagatttcat tgtaatctaa taatgaatat tcttctacac cagttttata atgagatgat
    19081 tgagcacaaa atttacagtc ttcactacat ttgccagatt tagcattcat tatcgtacat
    19141 aaatctgctt ttcttcccat aaaatattct cttatttcat ttgcagattt taatagaata
    19201 tcaaaatcgt ttttattatt tgtatcaagg cttattaaat taagagcttc ttcataactt
    19261 atttcttttt ctctcaatac tttatttttt aattttatta tgtatttttc catatatcct
    19321 cctatttgtc aacttaatat ttttatgtag tttacttatt atatatagta ttgcatatat
    19381 aacatttatt ggtcaacttt attttagaca caagtttact atacacaaaa aaatactgct
    19441 tcaaaataat attagtaaat ggattataca tattattatt aaaacagtat tttttattag
    19501 aatataattc ttactggttt ataaaactat ccttgtctta actccatcca agctttccat
    19561 tgtaataacc tctaagcctt catattctct aaatagccat tttaaatcct tcatggcttg
    19621 attaacctga ctttttgtta gattatttat aaaatattct tttctagttt cctcatactc
    19681 tttgctagtc aaaaagtttc ttagaaaatt aaacgcaaat acacttggaa tattgtttac
    19741 attagccttc ttgctgtttt ccttctcata gtaaattttc ataaatgcat actcctttga
    19801 tacttttaga tttctcttac aataattata tgcatttaag tgctactatt cttataattt
    19861 tattaattca tattctttac ttcccatacc catttcacat gcatagtcta ttattctagt
    19921 tccatttaca tgaggatgtt catgtttgaa aacatcatga cccatttcct tacaaatcaa
    19981 atcatagcat gcttgttcaa tagctacagg gtctgttgat gctaatattc ctatatcatg
    20041 ggcaattggt ctacctgacc aaggtacaca atcacaaagt ggagttacat tcataacaaa
    20101 agttatatac cctaccttgt cttttttatt tgatactgct ccataagcgt attctgccat
    20161 cttttcgaca aacacatctg agtcactttc ccattgtata gttactgctc tagttggaca
    20221 gacagttgga cattctccac atccataaca tacatcagaa tctataactg cttttttatc
    20281 cactatactt atagcttttg taggacaaga attaacacac tttccacaac ctacacactt
    20341 cttttccttt actactggag taacatcaga atgttgcatt tgttttcctg ctgcacttgc
    20401 acatcccata gctaagtttt ttaatgctcc tccaaaacct gctgcttcat gacctttaaa
    20461 atgactcatt actatcattg cagaagaatt ataaatttct ccacctattt ttacactttc
    20521 aaaatgtttt ttgtctattt ttacattctc ataatttcta ctatataatc catctgcaat
    20581 tattactggt gcatttacaa ctgcataagc aaatccattt tctatagcag ttgtaagatg
    20641 gtctacagaa tttgttctac tccctgtata cagtgtattt gtatctgtta aaaatggctt
    20701 tccttcgcag tctttaacct tatcaaccac ttgtctaact gccacaggac tcatatatgt
    20761 agtatttccc ttttctccaa agtgaagttt aattgcaact tggtcattct tttctattaa
    20821 atctttgaat cctgcttcat caaataatct tcttacattg tttggtacgt ttttattttg
    20881 cgaatttgag tataaatcac aaaaatatac ttttgacttt tccatatcta caccccttta
    20941 aaatatttac ttatactaat tttatcactt ttaaagtaat ttttagtata ttttaacata
    21001 attaaatatt cttatattta tttgatacaa ataatattat ttgtattatt ttctacactt
    21061 aattctatat attaaaaatc aaaaagactt gaaacttaag tccaagtctt tttgattttt
    21121 aggtgtagtt tttaacaaca taagattact ttaccttgtg ataaaggtat tattaaactg
    21181 gttttttatt aaataaaata acagctgcta gtaaacatac tacagaaata agaactgtta
    21241 taataattgg aataataaat tctggcattg ccactttccc tgaaatcaag tctatatttt
    21301 ttgacatcaa gaaattagga gtataaggtg caatctgttt aggaatagaa attaaactca
    21361 ataaaagagt tgccaccaat acaagtaaaa cactggcaaa tgctggtcta aatagtacac
    21421 acccaagaat taatacacta atatacataa ttcctataag ccataaatta aatgctgaaa
    21481 atataatatg attaagattt gcagttggcc aaaaatatat tgtatagcca taagcaaata
    21541 aaaaactcaa ccaaaaacca attgtcataa taatcaccgt aacagagaat ttagacaata
    21601 ctatagatga acgtgataaa cctttagtca gcataataac aagtgtccct ttagaatatt
    21661 catttgataa aatattacaa aaaataatta gagttaaact cataccaaga ccagagatat
    21721 tgctaaaaaa ttgtgtccaa gaatctaaag cagttggtgc agcagtaact tttaaagtcg
    21781 gtgcaaaatg agcaatcaag tctggcataa attttgcaga aataggacta cttataccaa
    21841 atattagaaa taaaataatt aaactaaata atttataatt tcttaaattc tctttaaatt
    21901 ctttttttgt aaatgctatg tattctctca ctttactacc tccaaaaata aattttcaag
    21961 tgataattct attagttcca tctttactgg acaaagattc atttctgcga gtgtagatat
    22021 aactgttttt tgtattgctt ttatatcttt accatgcaaa acaatttcca tattagtttc
    22081 ttctgaatct tttagtaagg atttaatacc atcactagac ttaaactttt gaatttcatc
    22141 attacttgca aactccagta aaagtctatc ctttccgtgc atacttttta tttctgaaag
    22201 tgtaccacta agggcaatgc ttcctttgtt taatatagca acatggtcac agattctttc
    22261 aacatctgat agtatatgtg ttgaaaatat tacagttgtt gagtctttga ttttaagcat
    22321 aatatctaat atttcttttc gaccaacagg gtcaagtgca cttgttggtt catcacaaat
    22381 aagtaatttg gggcgtgaaa gcaaagcttg agctatacca agtcgttgct tcattccacg
    22441 agaaaaccca cctattcgtc tcttttcatt tccaagaccc acaagtgata acaactcttc
    22501 actccttatc tgtatttctt tttttgaaag tcccgtaatt tcaccacaaa gagaaagata
    22561 ttcaagtggt ctcatatagt tgtaaaactc tggaacatca ggcagataac ctacatgacg
    22621 attagaactt gttttcccat aggtcacttt ctcaccacaa acatctatag ttccactatc
    22681 tggtttaagt aacccaagta ccattttcat tgttgtagtc ttacctgcac cattcttacc
    22741 tacaaaccca aagactgacc cttctggtac agtaaaattt aaattatcta ttatctttcg
    22801 attaccaaaa ccttttgata atccatttat tgagacaata ttcatcattc ttcacctctt
    22861 cctattgtaa aatacaaaat tggtcctatt agactaataa acagtactac aataatccat
    22921 attgccttat ttccaatttt ataatttgga tgtctcaata catgaacaag tgcagtaatt
    22981 ataagtatta aatctaaaat aataacagga accaaaagtg gtaagtattc catcaaattt
    23041 tccataatct tattcctcca ataaagataa tttttctata agaaatttgt attctttgtt
    23101 ttcacttaat acagaaaaca ttggattatt tttaatagca gcaacaatac ctgcttttac
    23161 gattgtatca tcacgtgtaa tacctgttcc aatttcagaa atccatctgt caagtttatt
    23221 aaaaaagtca tccccatgta ttataattgg gtatatatca cgtgttgcta aatctacata
    23281 ttttcgtaat gatttaatag ctccttcatt atcaccatgt attaaattaa tttgtgcctg
    23341 tgttaaatat gaagaaagca taatagcagg agataatgta tcaagctcaa atatatctga
    23401 aattgctaga atacgtcttt ctatttcctt catttgtaat ttatctgcat tttgcaaaat
    23461 acccactagg ttttggacta atgatataag gttctgatat gcaccaagtt gaaatgtttc
    23521 tctcgcttca tccatttgac cattcatcat ttgcccctgt gcaagaagta tagattcatt
    23581 tatcatggga aaattgctgt tttgcaataa atcaatcact tgtatgggtt gatttaacaa
    23641 tatataacaa gttgcctcca tactttttgc ttgacggcaa atatctatgt cattacaagt
    23701 ttcctgtata cgaataaata tttctaatgc ttcactaatt aatagttcac gctttttttc
    23761 atctacaata tcatagtgat taactattaa aagacccatc cgaaaaatca aaggaaaaca
    23821 tgaataatat ttttttatga tttctcttat ttcatccatg acttcatcaa atggtttaac
    23881 cgtaaagtct ttgcacagtt tattatacag tttattaata tcttccttca tcatttgagg
    23941 ttcataacat attaactcat caacggtaat gttgaaatat gtcgcgagtt gcggtaaaaa
    24001 agttatgtca ggatagcttt gtccagtttc ccatttagat acagaagctt tagaaacgcc
    24061 tatataattt gcaagttcat cttgtgtaat tcctttttct ttacgcttta aaatcagcgt
    24121 ttttgcgatg tttaattcat tcatgtcaat ccctccttat gtataattat atccttaaac
    24181 atgtaaagct aacaagcgat ttgaagttga cttttgacga aaaaatcaac cacagagaaa
    24241 caaataaaca agtatataaa atatatattt aaatttatct ttaatctctt ttgtaaaata
    24301 tgtaaacttt gtaaataaaa tccatattat ataaaactaa tagttaatct atctaaatac
    24361 agtttttatt tatcacgttt ttaaatattt tgtgtaaaac ctcttttaat ataaacacct
    24421 tattttcatt tctcatttca tattcttttt aaaatactga atataactct tcgattttcc
    24481 atttaattct tttataaagt tattattcgt atttatttct ctaattataa caggcaacta
    24541 tcatgtattg g
//
LOCUS       DAYAOY010000058        24320 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYAOY010000058 DAYAOY010000000
VERSION     DAYAOY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.058"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     regulatory      448..549
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            622..1965
                     /locus_tag="ACGI6B_002418"
     CDS             622..1965
                     /locus_tag="ACGI6B_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFG9751859.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            2144..3508
                     /locus_tag="ACGI6B_002419"
     CDS             2144..3508
                     /locus_tag="ACGI6B_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9751860.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3686..5551)
                     /locus_tag="ACGI6B_002420"
     CDS             complement(3686..5551)
                     /locus_tag="ACGI6B_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HFG9751861.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5787..7004)
                     /locus_tag="ACGI6B_002421"
     CDS             complement(5787..7004)
                     /locus_tag="ACGI6B_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9751862.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(7204..8067)
                     /gene="aroE"
                     /locus_tag="ACGI6B_002422"
     CDS             complement(7204..8067)
                     /gene="aroE"
                     /locus_tag="ACGI6B_002422"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFG9751863.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(8149..10071)
                     /locus_tag="ACGI6B_002423"
     CDS             complement(8149..10071)
                     /locus_tag="ACGI6B_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9751864.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(10135..10983)
                     /locus_tag="ACGI6B_002424"
     CDS             complement(10135..10983)
                     /locus_tag="ACGI6B_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFG9751865.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11396..12277
                     /locus_tag="ACGI6B_002425"
     CDS             11396..12277
                     /locus_tag="ACGI6B_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9751866.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12485..13324)
                     /locus_tag="ACGI6B_002426"
     CDS             complement(12485..13324)
                     /locus_tag="ACGI6B_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFG9751867.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(13503..15437)
                     /locus_tag="ACGI6B_002427"
     CDS             complement(13503..15437)
                     /locus_tag="ACGI6B_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HFG9751868.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15549..16562)
                     /gene="galE"
                     /locus_tag="ACGI6B_002428"
     CDS             complement(15549..16562)
                     /gene="galE"
                     /locus_tag="ACGI6B_002428"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFG9751869.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16586..17461)
                     /gene="galU"
                     /locus_tag="ACGI6B_002429"
     CDS             complement(16586..17461)
                     /gene="galU"
                     /locus_tag="ACGI6B_002429"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFG9751870.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(17653..17973)
                     /locus_tag="ACGI6B_002430"
     CDS             complement(17653..17973)
                     /locus_tag="ACGI6B_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751871.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17963..19954)
                     /locus_tag="ACGI6B_002431"
     CDS             complement(17963..19954)
                     /locus_tag="ACGI6B_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751872.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(20077..22620)
                     /locus_tag="ACGI6B_002432"
     CDS             complement(20077..22620)
                     /locus_tag="ACGI6B_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFG9751873.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22898..23232)
                     /gene="rnpB"
                     /locus_tag="ACGI6B_002433"
     ncRNA           complement(22898..23232)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGI6B_002433"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23364..24041
                     /gene="srtB"
                     /locus_tag="ACGI6B_002434"
     CDS             23364..24041
                     /gene="srtB"
                     /locus_tag="ACGI6B_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HFG9751874.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(24201..24275)
                     /locus_tag="ACGI6B_002435"
     tRNA            complement(24201..24275)
                     /locus_tag="ACGI6B_002435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24241..24243),aa:Thr,seq:tgt)
BASE COUNT         7778 a         4190 c         2725 g         9627 t
ORIGIN      
        1 ataattttat taaatatttt ttcatcatta aatatatctt caagtatatc ttcaagattt
       61 tcaataattt ctaattctcc tgtaaattta ggtttttgca ctttaataat tttagtcatt
      121 ttcccctcca ctataaatta tattattttt gatattattt tttttaatat attaacttaa
      181 tatacatatt tgatatatta aaaagttttt acgaaatatc catgtattca gtacttttat
      241 aattatatca caaactcatt tataataaac ttccattaat agtataaaac aagttagaat
      301 aattgacatt atactcatac atattcaatt attggaaaaa gtaatttttt tcaataaata
      361 accttaattt tttataaaat cgacaaaaaa acgaataatt ttattatatt tattgtaaat
      421 aatacaatat tacgatataa tacaacagaa aaacaaatag aaaatgttca tataatatta
      481 gtaatacggt ctaatagttt ctacaaactc accttaaatg ggttttctat gaatagaatg
      541 tcaactctga atatatgttc tattctatct atatgaatat atattttttt gtttatatct
      601 taaagttttt tggaggtaaa aatggtacca aacactaaca atgtacaacc aggtggaaat
      661 aattcatcat taagcttttt agacaaaatg tttaaattta gcgaacgtgg ctctagcctt
      721 aaaacagaag taataggtgg agttactaca ttcttaacaa tggcatacat agttttcgta
      781 aatccagcaa tattatctga agctggaatg gataaagccg cccttataac agttaccata
      841 ctagctacag ccattggtac acttatattt gcgattttag gaaatgcacc atttgctcta
      901 gctcctggta tgggattaaa tgcatttttc acatactctt tggttatagg tgcaggaatc
      961 ccatggcaaa caggtcttgg ggtagttttt ctatctggat gtgtcttctt tacactcgca
     1021 gtaacaggtc ttcgaaagac aattgcagac gcaattccta gagaattggc agtagcatct
     1081 tcagcaggta taggtctttt ccttgctttt gttggtttaa aaaatatggg tgttataata
     1141 gctaatccag atactatagt ttcacttgca gattttacac caacagtttt aatagccctt
     1201 tttgctctta tcttaatggc aatactagaa tataaaaaag ttaaaggtgg aatattgata
     1261 agtatagtag ttgcaacaat aatatctgtt atatttggat ttgtggagtt gccaacagca
     1321 ataatctcaa cacctcctag tatagcacca gttgctttta aattagatat tgtaggtgca
     1381 ttgaaattct cattaattgg acctatattc tctttcatgt ttatagacat gtttgattca
     1441 ttagcatttc ttattacttg ttgtaaacaa atgggtcttg aagatgagaa tggtgaaata
     1501 aaagatttag gaagaatgtt atatgcagat gtttcttcta ctttaatagg ctcattctta
     1561 ggaactagta ctgttacaac atttggagaa tctgcttctg gtattgcagc aggtgcaaga
     1621 acaggacttg cttcaatagt aacagcagca ttattcttag ctacacttat atttacacca
     1681 ttacttggta tagttcctac ttacgctgca gctcctgcac ttgtaatggt tggtgtgttt
     1741 atgtttgaaa gcataaaaca gattgatttt gctgatagca aaatagctgt acctgctttt
     1801 gttacagttt tattgatgcc tttaacttat agtataagta ttggtttatg ctttggattt
     1861 atttcatata taataatgca tgtagtagct aaagaaactg ataaaatcag tattacactt
     1921 tggattatag gtttacttgc agttgtaaac ttagttctaa gttaatattt ttaaatagtt
     1981 ctaggtcaat attttaaatt atatagaata aaatcttatt tattttaaat ttataaatag
     2041 caaaatcact ttatatgtaa attgattttg ctattaactt tgtatagaaa atttattatc
     2101 ataatatata gatataaaaa tatatagaaa aggagtggtg tttatgtcac aattacttat
     2161 aaaaaatgca tatctaatta ctatgaatgc agatagagag gtatatgaaa atggaagcat
     2221 acttattgaa aataatatta taaaagaggt tggtaagttt gatattaata ttgtagataa
     2281 agatgcagag atatatgatg ctaaaggaaa aatactaatg ccagggctag tcaatactca
     2341 tgttcactta tctcaacaat taggacgtgg tcttgcagat gatgttgtac ttttaacttg
     2401 gcttagagaa agaatttggc catacgaaag tagcttcaat tatgaagatt ccttgatttc
     2461 atctactgca tgttgtgttg aattgataaa atctggtgtc actacatttt tagaatctgg
     2521 tggtcaatat gtagatgcta tggttgaagc tgttgataaa actggactta gagcctgttt
     2581 agctaaatca gttatggata caggagatgg tcttcctgag gcatggcaaa aaactactga
     2641 tgaggaaatc aacactcaat tagacttatt ccataagttt aataatacat ctaatgagag
     2701 aattaaaata tggtttgcat taagaactat ttttaataca actgaagaat tacttgttag
     2761 aactaaagaa ctagctgata aatacaatac aggtatacat atgcatattg ctgaaatagc
     2821 tgatgaaatt tcatttgtaa agcaaaacaa tggagttgga acagttgaat accttgataa
     2881 actaggtgta ttaggaccaa atcttttagc tgctcataca gtttggctta ctcataagga
     2941 aattgattta tttagattac atgatgtaaa agtttctcat gacccaggtt cagctatgaa
     3001 agtggttcta gggtttgcat caattcctga aatgttagaa aaaggaatac ctgtttcaat
     3061 tggtactgat ggagctcctt caaataatag aatggacctt atgagggaca tgtatcttac
     3121 atcactaata cataaaggaa gaactttaaa cccaactgtc gtaccagctg aagaagtctt
     3181 agaaatggct actataaatg gagcaaaatg tgctttacta gaaaaagaaa ttggtagtct
     3241 tgaagtagga aaaaaagctg atatgattat tctaaatcca gatacaattc attgtttacc
     3301 aatgcataat ccaattggaa atatagttta ttctatgact agtgaaaatg tagactctac
     3361 tatttgtgat ggtaaatggt tgatgaagga aagaaaagta ttagttgtag atgaatctga
     3421 attattaaat aaagttaaaa aacaagcttc taaaataaaa gataaagcag aagttaaaat
     3481 tccatctaaa tttaaaatta taaaataaca taaatttcaa ttgttttgta aatatgaata
     3541 tataaatata tttatataca tcatatatac aaaacaattg tttttttatt ttatattcat
     3601 tagcattgct cataaaatac accataattc cagaaaaaat aaccaatcag ttaggattac
     3661 atcatctaag caatatataa acgtcttagt ttataagttt tattctactc ttatctaaaa
     3721 ccatctttaa agagttttca actgtttcaa ttacatcttt ataactcaag tacacatttg
     3781 cgtagataca atcttcatat aaattatatt cagttactct acaatacagc agttttcctc
     3841 catctaataa attgctcaaa atatagtttt cttttcttgg gacatagcct atcacatatc
     3901 cagtttcacc aactatttta atagcttttt catcatatag attatctttc tctctagtta
     3961 aatagtatat cttacttttc tccaattctt tttcaagata ttctctattt ctaaaccttg
     4021 aaccagcaac agctattgaa gtcaaataga tatccttacc atgttcatct acttttattt
     4081 tttcaatatt tacaaattct ttttttaaat tattactttc atataatact cttcttaatt
     4141 catccttcaa tataacattt ttttcttttt catacgcttc tttaacaatt tttaattctt
     4201 caagagatag tttctctctg tatcttttta agatatttat ggattctttt cttacttcat
     4261 ttatatttcc aaatattcct tttaaagata tattctttcc ttcaggatat agtgatttac
     4321 ttcctttaag cacaataaaa aataacatat catcataata ttcttgagtc aatttatctt
     4381 tttctatatc tttcattttt ccaattaaat catctatttt aaacgtctct tcaaaaaaat
     4441 ttaataattt taatctagaa cttgtcgttc cctttttaga tatatgccaa tatacctcaa
     4501 aatctctaat atctctattt agatatggca acaagtcatc aaaagataag tttacatcat
     4561 aaatttctga catttttata atatcttctg tttttccact agcatctctt aatgcttcaa
     4621 aatatatatt tttccatttt tcactgagca agataccatt tatttctttt tcaaattcaa
     4681 tcttattgca ctctatattt tcatctttta agacgtttat agctattagg aaaattgaat
     4741 ataaactttc aaagttagtc ccataattta ctacagtagg taaaaatcga ttcactaatt
     4801 ctaatttatt acctatatat ttaaattcta cacttagcaa gtaattgcat ataagacgtg
     4861 ctaaattatt tatcttttct ttatctaaat ctcgtttttc aagatattca ttcaaatcta
     4921 caattgaaat aatatagttc atcaataatc gttcgtactt tgtatcctta taaccatctt
     4981 caattagata ggaatttatt ttagaatcta aattttccat attaacaata gcaaatactt
     5041 ttattgaacc tgtagccttt ttagataaat taaataaata tgtattgtaa aattcaagtt
     5101 ttttaatagt attactcaga taaaatacgt attctccaga tttagaaaat gtatctacaa
     5161 cttctcttaa gttttctaca tttagatatt tctcacttaa aactaaccct agttttacat
     5221 actcaacttt tttactctta tatattaatt ctttagttaa tttatataac catttctcat
     5281 caataatcat ttcttttacc aaagtattta tctttttcat aaaaatttcg aaataacaaa
     5341 ttaaagactt agaattacta ccctccaata aataaatttc attagattca cttttataca
     5401 tatttaaaat ttcatctact agattatttg caagctctac ttgttctctt aacggtatac
     5461 tttcatatat acataggtaa ttcatttcaa atgattcttt gtatccatca taaccattaa
     5521 attcctgcaa tcgcttaaaa acattctcca ccaaattatc ctcctttaca tgatgtaatt
     5581 tatattgatt atactactta tttcatcaag tttacattta tacgcaaaaa aatattacta
     5641 gaaattgaaa ttctagtaat atccttatta ttaattatgt aaatacccta tcttataact
     5701 gtaaataata tccttattaa ttttaattac ttttaataaa aatctatcct agatttagac
     5761 ttctctatgc atatattata ctaatattag acatcataat catacacttt ttctttttca
     5821 tatctcatgt ttacaaacaa tgcaagtaaa attccaacaa atgttattgc cacattaaac
     5881 aatacaacat attttggtcc attaactccc cctgattttg taataatacc tgctatattt
     5941 aaaataacat agtttgctat actagatgca atcataacta tagatgttat ttttccttta
     6001 ttagttggaa acatttcatt tgcagttgac gttgttaact gaagaacacc tcctgctgct
     6061 gaataaccta ttacaaatcc ccctaacata caaatagttg gagtttgaac aaaatataca
     6121 actaatagca taagtaaagc tattatagga tatataacta atattcttat tggtttaata
     6181 tattttttaa taagtaaaga agttattaat actgcacata ttactcccat tgagtaaaag
     6241 gattgaattt ttgtagggtc agccatccca tacaatttac ccaattcctg attacaattt
     6301 aaccataata caaatgtaga tgtacatgta aatccaatac aaacaagagc tatacttgtt
     6361 ggtgtaaatt tcatcttctc tgatttaact gttttgcctt tgttatcaat aacattatta
     6421 gctggtggaa atggtaacac agctatcaaa aaggcatcta ctacaataag aatcgctgtt
     6481 actataaaaa taactttaaa tgatattgaa tttgctgcaa caaatccaat tataaaagga
     6541 agcaaaaatt gtgctaaagc tattgtaaat ttagtaaaca tatttgctat tgttcctaaa
     6601 ctagcaaata tctccataca agaaggagtt acacaggtat ctaagaatga atttgctgcc
     6661 cctccaatta ttgcaaatac atatgctata taaaaatttg gtgaaaatac aattccaaca
     6721 aagaaaattg catgtagaaa gtttcctata agaccactta cttttcttcc atacttatct
     6781 gaaataggtc ctgcaaatgg ataagatata agtctcccta atcctagtgc agcaataaca
     6841 gctaaaacaa tacttacatc caatgttcca tctgataatg ttttagcccc ccacattgat
     6901 gaaaattctg gtttgtattg tcctaaaatt gaaattccaa taccaagtaa aaaatatgaa
     6961 aaatacaacg ctatggcagt aggataatat tttttattgt tcataatttt tctcccattc
     7021 ttgttaattt attaacattt ttgtgtacaa taaactcatc cccataatgg atttaaagta
     7081 tagtcagtta aagcttcaaa tttaactgga ctatgaggca aaacccacta tgacaatttg
     7141 taatttatta ttaaaaatat tgaaatacgt atgttttttc attgtttaag atattactct
     7201 tatctattta aagcataatt cattaacttc ttctacaggc atttctaatc cagtatatag
     7261 attaaaagct tctgctcctt ggtataataa cattcctaat ccaccaatag ccttacatcc
     7321 attagcttca gcatcttcaa tcatttttgt tttcttagga ttataaacga catctgttac
     7381 tactaaatct tttcttaaaa cactagtatc ttttatattt gtttcattgt cataaggctt
     7441 cataccaata agagttgcat ttgtcaatat atcacttgat tgtatctcct cataaagttt
     7501 gtttgtatct tctaaatcat atagattaac aacacattct ggtacatctt tttttatatt
     7561 ctcaactgtt tgctctgctc ttttataaaa atcatcctta ggattaaaga tagatatttc
     7621 tcttgctcca tctaaagcac attgtacttg tatagcagtt gctgcaccac cagcacccat
     7681 aattgtaatc tttttacctt taacttctac cccattttct tttaaattac gaacatagcc
     7741 tactccatct gttatatgac caactaactt tccattgtca tttacaattg tattacatgc
     7801 tccaattata cgagctgctg gagaaagttc atccatatat tctgtaacag cagatttaca
     7861 tggcatagta acattggctc cttttatatt aaaagttttt attgctaaaa gtgcctcttt
     7921 taccttatct actgtgatat caaaggctaa atatctataa tctaaatcta atttcttaaa
     7981 actataatta tacatcactg gtgatttaga gtgaccaacc ggtgtaccta ttaatgcaaa
     8041 taatcctgtt ctacctgaaa tttccataac tataatctcc tctcaatatt atcattttct
     8101 ttgcaaataa cctattcagt tatttacaaa gaaaaattta tttattattt atatatttaa
     8161 tgctacttca aaagcttctt ttgttgcatc taatgcccta cgagctttta ctgcatcacc
     8221 aacaatataa gtttctttta caacttcttt tataacttca cttaatgggt cgtaattacg
     8281 agaacccata gcaagtacta ctgaatcaaa accttttaaa tcatgctctg tgccatctgt
     8341 aagtaaatag tcgacaccat catcataaaa tttagaaact tttgcaccag taatggtatg
     8401 tatattataa ttttcaaaat cattcataag gaatttacgg tgttctgaaa ttacatctgc
     8461 accaacttct tctctaagct caataacagt aacctcatgt tcttgttctc caaggaaggc
     8521 tgccacttca catccaacca ttccacctcc aacaacaagt acttttttcc cacaagattt
     8581 tttaccatca agaaggtcta cagcatgaat taatcctgaa tcattgattc ctggtatagg
     8641 aagtacaagt ggagttgctc cagtagctat aataactaca tcaggtgatt tctcctttat
     8701 aagttctact gtagcttctg tattagtaca tatttttaca ccatattgat tacattttga
     8761 aatataactt ctaactaagt tagtaatatc tcctttccct ggaggatatg cagctaaacg
     8821 catttgccca ccaagaactt cagatttttc aactaaagtt acattatgtc ctcttttagc
     8881 acatacccat gctgcaagca tacctccaac acctccacca acaacaagaa cttctttgct
     8941 aatttcagct ggcttaagct ctgcttcatg tcctaatata ggatttgcaa gacaggctat
     9001 aggtttgcct tggaacatat ttggtacaca tccttgtaag catgcaatac atggtatcat
     9061 ttcgtctagt tttccatttt tagctttatt aggcatttct gggtcagcca agctttgtct
     9121 accgaatgct actaaatcac atcttccttg tctgaccatt aactcagcaa actgtggctc
     9181 tgtataacgt ccaactgtta ttactggtat actaacagct ttttttattt cagtaactaa
     9241 ttctgcactg aatccagcat gtaaagtcgt aggagcccac atatattcat catgtatatg
     9301 tatactacgt gaaacatgaa gtccatctat tccacaatct tctagatatg ctgctattac
     9361 tgaactatcg tgtacatcaa ttcctcctgg aacatcatca gtgctgttta tacgacataa
     9421 tattgctaga gaatttccta cttttttacg tatattttca atgataagac gaggtaatct
     9481 cattctattt tcaaaacatc caccaaactc atctacacgc ttatttgttc tttgtgatat
     9541 aaaactacta accagatatc catgagcgca gtgaacttca acagcatctg caccagcttt
     9601 ttgtgcacgt aatgcagcat ctccataaga ttctataagt tcatatattt cttctgttgt
     9661 tattgctagt ggtgtattac gaccatttga tgcaggtatt gcagaagcag cttttaatgg
     9721 atgtcctgct actttttcat ttccttcaga accagcatgt tgtaattgta tagatacctt
     9781 cgaacctgca tcatgacaag catctattac atttttaaaa ctatctattg tgctatcatc
     9841 atataaacaa ggcttacgag gacctccttt tgcttttttg tctacaacac tagattctat
     9901 agttattaat ccaaaaccac ctaaagcacg ctcaagataa taagccttag aagtttcact
     9961 taaagtacca tctgtgtttg caaaattgtt tcccataggg ggaactacaa aacgatttgg
    10021 aactgtcatt gaaccaattc ttataggtgt aaacatagaa ctaatcttca taaggttttc
    10081 cttcctttca agttttatat tataatactc tgtatatttt tatattatat aagtttattt
    10141 tggagatact tcagaccatg ctttatccaa aactttttta gttgcattaa atacagtttc
    10201 agcagcagtg gataaccctt tagatattag agaatcagat attacttctg cccctatgac
    10261 tcttggtgat atattgtgtt cttttattat tttcacaaat tcctctgtgt ttccatatcc
    10321 ttcacctgga gccaaacggt catgtagtga ttcatcccta agtttttcat aaggagtttc
    10381 taaaacatca cacacttgaa ttgatactat cttatctgct ggaacaggtg caaggtcctc
    10441 tgctgtctgc tttgctctag cccagtgcca agtatctaat ataagcattg cattttcacg
    10501 attacatcct ttcacaacac gccatgcagt agccaaatct ggtacaccac tatatggcat
    10561 aaactcaaga cctataataa gttcacctgc tctgtcacat aattctccca atgcagttat
    10621 aatttgttct tctggtatca tctctaataa tccacaattt atatgtttaa cattaaataa
    10681 gtgagccata tgataaacat tttgctcttt ttctctttga gcttccgttc tatcttcttc
    10741 tcttccccat tgagttatat attctacttc tgtaactttc atatcatatt cattaagaat
    10801 tgcaatcatt tcttcatctg taacacctgc attttttgca tcaatataat tttcagcacg
    10861 caatccaata ccatcaaacc cagcttcttt tgcaatacga actctatcac ggaatgaaac
    10921 ttctgttccc aatgtataag aactaattgt aattggaata ttaagatttt tttgattatt
    10981 catagcttaa atttgccccc tttattttct ttataattaa tagtattttt gattttatat
    11041 ttttatttat cctactagta gtattatttt accttaaaaa taaaagtatt ttttgatttc
    11101 tatttatcta gattttataa atttattgta tcacgagact ttactaataa caaattgata
    11161 atttcctatt aattcataga ttttgtttat agattttatt tataagataa ttcttgtatt
    11221 tttctattag gtcgtataaa tatatttatc ttataattca gacaaaaaac gataaatttt
    11281 tttaaaagct tttattctta ctctataatc atatataata aaaatgtaaa aatatcttaa
    11341 aggttaactt ctacaaatat ataaataaaa atacctatga aaggatgaac aaaaattgaa
    11401 tttatatcac ttacgctatt ttgttacatt ggcacattta gaacactata caaaagcggc
    11461 agaaaattta tctataacac aaccaagctt aagtcatgct atttctttac tagaaaatga
    11521 acttggggtg gctctatttg aaaaagaagg tagaaatata gtccttacta agtatggaaa
    11581 aatattttta aaagatgtgg aaaaatcatt agaaatttta gattcaagtg taaaatcatt
    11641 aaaaataact ggtactggtg aaggacaaat agacttagct tttcttagaa cacttggtac
    11701 tgattttatc ccagacatag tgcacaaatt tttaaaatct aatcctgcta aatcaataga
    11761 ttttaaattt catactggtg taactactga cattattcaa ggtcttaaag aaagaaaata
    11821 tgatattgca ttttgttcga aacttgaaaa agaaaaggga attgaattta tacctgttgc
    11881 caaacaagat ttagtactaa ttgtaccata tagtcatcca ctagctgcaa aggatactat
    11941 agatttaaaa gaaaccatac cttatcctca aatagtattt aatcagagaa gtggtcttag
    12001 atatataatt gatgatatgt ttaaaaaaat aaatcaacag cctaatatag tctatgaagt
    12061 agaggaagac caagtaattg ctggattagt tgcaaaaaat ttcggaattg ctgtagtccc
    12121 aaatatgaat atgcttagct ttacaaaagt aaaagtaatt cagattattc acccaagttg
    12181 ggaaagaaac ttttacttag catttataaa agatagatat ttgcctcctg caattaaaaa
    12241 ctttaaaaac tttgtcatta aaaacgctca attataattt attaataaag gggttatttt
    12301 taaatatcct cttttattct ttttataaaa taaaaaaatc tatactttta aaagtataga
    12361 ttcctaactg acagtcggca tccgagtctc agtaattatc gttatacaac agcaactgga
    12421 ttcagttcct gaataactag gttacttaca ttatatcaga ttttttaaaa tttgtcaatt
    12481 gtatctaacg cattttttca agccatggtc caacaggctc tacaatagga acttctttaa
    12541 atccccaatt tcttatatca ttcacactcg ttggagtata tattttgttt ctgacagatt
    12601 tgaatatttt gtttttttct ccatattgca tacactttgg attagcttct attaaccctc
    12661 ttcttgctct gctaagagga ttacataaaa tatctgctat ttctaaacct tctctatagt
    12721 cagcatcttc ttttttggca attataaaac ccttaacctt atctcttaat tcttcatcta
    12781 tatctaagta tattgttcca ttattaaata catcaaaaaa cgccttttgt acttttaagt
    12841 ttgagacatc atcccttgac tctataacta ttcgtgcaga ttctacagtt tcattattta
    12901 caaatgaata aaaattctga agaacatgta taaaagccac aacatacggt tcctttgaag
    12961 gtgcataata tttctttaat ctagctttat ctactgttac tgatattata gtacactcta
    13021 atctagatag aaataaaggc aactcatcat agaataactt aatctgtgaa tcatcaaact
    13081 tctcaaatgc tcttgatttt cttgatatat ctgtcaaatg tattcctatg ttactggttc
    13141 caaaacaata ctctttaaat gcatccattt ctttcctcaa agaacataca taatcgcctt
    13201 gacttactgc atatttatat tcaaaaatta ctcctgtcaa tgtaaatggg tcttcatgat
    13261 ttatatcagc tcttccactt tcatcaataa acattatata ttttttcttt ataatcttac
    13321 tcaccctctt aacatatgat gatagcatct atattcattt tatatgagtt ttaaaaatat
    13381 tccaagattt ttatataact taaacttaat tttatactaa aaaattgaga tgtaaaacaa
    13441 tcaagctttt attgattgaa tttcacatct caattataat tatttttcaa tatatatttt
    13501 tactattttg caagaatatc cattaattta tccattacta tataagatac tccgcctcct
    13561 atttgataag gagtttcaac ttttttattt gcaaatactc tttgttgctc tgatgataat
    13621 cctgaattat ccattaaaac tattggtgaa tgtaatttta cagctaatgg tccagctgat
    13681 aaagcatcta ctaaagcatc tgatttacta actaatacag cctgtaaatc agtatctcca
    13741 tagaattttt ctatcacttt ggcattagta tctactctac tatctccata tattctattt
    13801 cctgaaatat cttcagtagt tatatcattc atttttgcta ttactgaatc gttaattgca
    13861 cttggtccac ctataaagta tgcattttct aattttcttt ctgttagcca tttataagat
    13921 tcttctgtaa ttccatcttt attgactaat attataggtt gtttttcttc cccagcttta
    13981 gaagctacag ataaagcatc tgcctcccca tttccagaaa ccatatatgc agtttttatt
    14041 tcttctcgat ttccaagttc ttcagctatt cttgtagcag tttgatatct atcttgacct
    14101 gctattctct ttgtttctgt gttgaaagta gtttttattt cttgctctaa tttagaactt
    14161 attgcatttt ctccacctat caagattact gttttaggtg ctaataactt catttcttca
    14221 acagtttcag ttggtaaatt agctgtatta gtaagtagta ttgttgagtc atagctactt
    14281 gctaaaggag ttgctgttat accatcagct attgcattac catttactaa tattacagta
    14341 tctgaactat cagcattcca gtttccaact gaacttatct ttttagctgt atcaaatcta
    14401 tttgcaccta taattgtatc ccatgtagga tttatagttc catcttcatt aaaagtataa
    14461 tctttcccat ctattttttg agaaccagtt agcatctttc catctttgtc taaatagtaa
    14521 gacttaccat ctatttgttg ccatcctgtc tgcattattc ctgttttgtc aaagtaatac
    14581 ttattaccat ctatttcttg ccatcctgtt actgctaaac catcagatcc tagatagtat
    14641 ttctttccat tcttttcttg ccatcctgtt tgcattactc catctgagtt aaagtaataa
    14701 gtttttccgt ctattacttt ccatccagat actcttgctc catcagttcc taaataatat
    14761 tttttgttat taattgtttt ccatcctgtt tgcattactc ctgaagcatc aaagtaatac
    14821 atttttccat ttacttttag ccaactattc ttttggtatg ttccatcaga ttttacataa
    14881 tacttcttac cattttctgt tttccatcct gtttgattta gatttatctt atattttgcc
    14941 gctatcttta catagctgtc gttactataa tctataatta catctgaacc tacaggaact
    15001 tggtcaaata accatctaac atctttattg tgcattctta tacatccacc agaaatatgc
    15061 tttcctatag aactctcatt attatttcca tgtattccat atgtatctcc ataagttcct
    15121 tttaatgcta atcccatcca tctatctcct aatggatttc ttggacttcc tccaggtata
    15181 ttacctttat aatatggtct attttttatc ttgttaacta cttttgtttt ccctgttggt
    15241 gtttctgacc cctttttacc tgttgcaact ctaaattctt ttacaagttt attatttaca
    15301 aaatatccta gagtattttt tcttgaattt actataatca catgtttact ggctgcgctt
    15361 gattctggta cgccacatgc aaatactaaa actaaagtca gtgctagtac taaatacttt
    15421 tttagacttt ttttcactat aacttccccc tcttttagaa acattactac atgtgtctaa
    15481 ttataacata aaattaccaa taccttatat ttacaaattt gttacattta tacatataag
    15541 tatgtattct actcttcata cccattagga ttcatacttt gccatctcca tgaatcttca
    15601 cacatctctt caagttcata cttagctttc catccaagct ctttttctgc ctttgaagaa
    15661 tctgcataac acatggctac atctccagcc cttcttccta caatcttata tggtatttct
    15721 tttccacttg ctttagaaaa tgcttttact aaatcaagaa cactataccc ctttccagtt
    15781 cctaaattat aaacaacaag tcctggattt tcttctaatt tttgtaatgc ttttacatga
    15841 ccagcagcta aatctaatac atgtatatag tctctaactc cagttccatc atgtgttgga
    15901 tagtcattac cataaacact caattccttc aatttaccta ctgctatttt tgttatataa
    15961 ggcattagat tgctcggaac tccatttggt tcttcaccta ttctaccact tttatgagca
    16021 ccgactggat taaagtatct taaaagagct atatccaatg acttatctgc ttttgatata
    16081 tctactaaca tctgctctat cataagttta gttcttccat atggattggt tacagaaagt
    16141 ggaaaatcct ctaatatagg acaagtatgt gggtctccat atacagtagc tgaagaacta
    16201 aatacaaact ttttaacacc atattctctc attaattcaa ataaatttaa ggtacttatt
    16261 agattattgc tgtaatattc aattggtttt tctactgatt caccaactgc ttttaaagct
    16321 gcaaagtgta taattgattc aatattattt tctttaaata caacatgcat ttcatcttta
    16381 tttctgatat caatattata aaattttact ggttttttag ataattcctt gattctatca
    16441 accactattg agttgctatt acttaagtta tcaactataa caacttcata acctgattct
    16501 aaaagttcta ttgctgtatg gcttcctata tatcctgctc ctccagctac taaaactgcc
    16561 attgtaatta cctcttttct aatatttatt tttcacttac tttttttctc atatagtcca
    16621 ttaaatcatc ttttaaattt tctctcttta gagcaaagtc tattgttgcc tctaaaaaac
    16681 caattttatc tcccacatca tatcttctac cttcaaagtt ataagcataa atagcctctt
    16741 gttttccaag agtttgaagt gcatctgtca gttgtatctc tccaccttta ccaggtgtct
    16801 gtttctctaa tatgctaaat attgcaggag ttattatata tcttccaagt atagctatat
    16861 cagatggagc ttcttcaatt gcaggttttt caaccatatc tttaacttta tatactctat
    16921 cttcgatatg ttttacatcc aatataccat atttattagt atcttcttta gctatctttt
    16981 gaacacctaa tacagttgtt ttatactcat tataagtatc tattaattgt tttaagcacg
    17041 gaacatctga atctactata tcatcaccta ataatacggc aaaaggttca tcacctataa
    17101 aactttttgc acagtaaatt gcatgtccaa gaccttttgg ctctttttgt cttatataat
    17161 gaatatttac catatttgat atgtctctta ccatttctaa catctctgtc ttaccttttt
    17221 gctctagttc taactctaat tcaactgatt tatcaaaatg atcttctata gattttttat
    17281 ttctacctgt tattataaga atttcttcaa ttcccgattc tatagcttct tctattatat
    17341 actgtaaagt tggtttatct actataggaa gcatttcttt gggctgtgat tttgttgctg
    17401 gtaaaaatct agttcctagc cctgctgctg gtattattgc ttttttaact gtcatctgca
    17461 ttttttcacc cttcttcgta aattttctat tgtaccaaat taatatacca tatttatact
    17521 ttttcttcaa actctatatt atcccaatat ttaatgctat aagataatat aaggtagatg
    17581 aaatttcatc tacctttagt tttataagtt aatattgtat tgtttatatt ttatccaaaa
    17641 cttatacagg tactaaattt catctaagat gccttttata tcactatcta tatcttcatc
    17701 tagataagaa tctaaatctt ctaagtcaac ttctagttct tgatatgtca tatcaacact
    17761 gacatcctca tctatatcca tttctgatat taattctcct aaaatactat ttccatcaga
    17821 atctaaacca ctatctatat cttcatctat atagttttca tttaaaattt catctaaatc
    17881 aatctcgtaa taaacttcat tatttttttc atcccctgag tccaatccta agaactcata
    17941 gttaaaagtc caaacattgt atttacttga catactttat tcctcctgtg aaaaacatat
    18001 tttcatctag tttattggtt ccataatcaa cagagttata tattttttca attatgtctt
    18061 ttcttctttc ttttgtacta ttatttaatg tcaattgttt aaatacatat gtatttgtta
    18121 ttctctcgaa taatatttct tttatactat catcatcaat atttctatag tttttccaca
    18181 ataactcaaa taacttaaac atttccctac tagtttcatc tacataataa ggatacatat
    18241 tttctactaa atggtttaat cttgatatgg cttgttttaa aaatacatca agacttgcac
    18301 tttcactttc ttttaacatt ctataattgt atttcttctt atcttctcct gaaattacaa
    18361 agatgatatt gtctaaactt ttttcaactt catttaaata actgcttata ttatatgata
    18421 aatcagacaa tagattttta atctgcttaa acacttgtac atcaagttta gtattatact
    18481 tttcaaacaa atctatttct tgattttcta atattcttgg catatatgct gacaataaat
    18541 ctaaattgtt tctttgttct tgtatttgct tataaagtga atttataact tctattctta
    18601 tttcatcatt taactctttt tctaaaatct tatcaaattc agatttcata tagtaaatat
    18661 ttccaaagaa attaaatatt atcatatcat tcatagcttt agaccaattc ttatatatca
    18721 cttcaataga attttcttta agtagaggtt gctttgcata tataaataca tcttgcataa
    18781 gtttttgaac catactttta cttaagttta aaagtacttt ttgattatta aattttattt
    18841 ccgtttctac aaaattagaa ttttcgtttt caagttcttt attaattaca atgtctttta
    18901 tatcactatt ttttatagat atgatttctt tttgaaaaga ctttagaata gtcaagctat
    18961 cgaaatcttc ttgaataaaa aatggagcat gttttaatgt atatcccact atttcatttt
    19021 ctatatcttt taggtcatct atttgcataa tttctctatt tttatcattt atatgtaggt
    19081 taaatacatt tttattatca tataatatta tatcttctcc taaatttaac ttagaacaat
    19141 tatctttcgc tgactttaca atagtcttct tagatttctt atttataaaa attaattggt
    19201 cttgatattt tactaaaagc atgtttgtgt ttttataatc aaatccatct atatttcctg
    19261 atatcttgca gaatactatt tcacaatctt taggaagatt tcctattttt tggttatctt
    19321 taactctctc atttaaatca gatgggacta cactatctat attgttatta aatatagata
    19381 cagaccttac tataatcccc tctctttcca tatgaaagta tcccttcaat gacactcttt
    19441 tatcttctaa aatgtatctt tctatatcat caaaatttat ttcaaatcta tttttcccac
    19501 tgataaatat aaacttctca ttatctttat taattccacc catataaaac ttattgtcat
    19561 ctaaacttaa tatagtcata ttatccttta gatagaatat aaattctctt tgacttttgc
    19621 agattaaatc atattcatcc aaagttattt tcatagtcaa atttgctgta acatttttac
    19681 ttgaattttc actttttatg taaaaaggca agttatttcc atagacaatt atttgagaat
    19741 cttctaaaac tgcctctaat ttttctttat tgtctgaaaa actatatata tattttacac
    19801 ctgtatcctc attaacttca tctgatttgc tgattattat attccctaga tttgttatat
    19861 gaagcttttt atattctttt gagtatctac ttatgccact aagctcaaac ccatctgaaa
    19921 gctcatatat gattttttct cctaatttca acataccttc accacctaaa cagaattata
    19981 agtattttat aaaggtgaat aaaatgtctc aaaacaaata atattttttt taatactttt
    20041 tattctgaga catagtttct ccttttttat aatgctctaa gcaccttcaa ctatttcttc
    20101 ataaagttta tctaaatgtt taacatattc acgatatcca gatttgttct ttaactcact
    20161 tcttaaatct tcatattttt gtttaagttc ttcagcactc aaagaaggag ctgattcttt
    20221 taattcttct aacttataat catagtactt atcatacata gctatatatg tatcaataga
    20281 cttcttagct agttcattac atttttcttg tgcttctcca tattgagaat aatcacctat
    20341 ttttatttta tagtatccat tttcttgaat tattgttgaa gaaatacctt tagcttcata
    20401 ttcttttgct gtttcttttg ctgttttttc aactttaagt gaacctgcat aaacagtgta
    20461 atatacacct ttaccatctt tatatgcttc catttctttt gctttctttt cagcttgtgc
    20521 tttttcttga gcctcttttt cagcttttgc cttttcttct gcttcttttt tctctttttc
    20581 agcttgtgct tttttctctt cttcagttaa tttattgtta gcttgttgtt gagttgatga
    20641 ctttggaata ttctctttat catttttctt attgttgcct actgttataa aatatcctac
    20701 accaacaata accactataa ctactgctat aatagtttct tttaatctac tcttcttaga
    20761 aacaacctct tcatcatcta aagaatcttc tttatctgat acataatttt tcatttcttt
    20821 cttgttttct ctatatttgt ccttagaaga atttttagat tttttattac tattttcttt
    20881 ctttttaata ggtattacta ctgatttttc attttctaat ttttcttctt ttatttcttt
    20941 tgctttttta tcttctaagt ttttactttc tttttcaagc ttcgattcat tatttgaaga
    21001 tactttttca tctaagttat tatttattgg tttttctatt tttacactac gttcttcttc
    21061 tatatgttga cttggttctt cttgttttat ttctttcaat tcattttgct ctatttcttc
    21121 tgatttcact tgatgatttt caactggttt atctaaaatt atatctggag aaatatcatt
    21181 taaattctct tcttcttgtt cagcttcttc attgtctttt ttaataaatc caaataattt
    21241 agccttcttt ttactctttt tattttctgg ttcaattata tcttctttat tgctgtcgta
    21301 ctctttatct gtagtatctt tatcttctac aacgtcacct atatcttgat tattactatc
    21361 tacttcttct ttagtccctt cttcagcatc atcttctatt gaaatactac ttctaaagca
    21421 aatatcttta tctgcatttt ttatcatgta aatgtattct tcaatatcga tatctttatt
    21481 tcttctagtt ctttttgatg caatttcatg attttctaat tcactatagt tatcttttat
    21541 tagattctgt gctttattcc atgtatcttc actaatttct gttttatttt taattacata
    21601 gtccacaaat tccttaaaat tttcaggacc aaattctcca attatagtca gtctcattgc
    21661 accttctata aggtctctat ttcttctctt taaaaattct aatacaactg ttgcacaagc
    21721 aaaatcttta ataatttctt tgccaaaacc atatgcttca acagaatgaa gttcttctaa
    21781 tccacataac ttcttatatt cattgtgtat tgtttgagat atactttcat tatttaagct
    21841 atttaactta tctataaact tcgattctat atcatttgat tttgattcat gttttacttt
    21901 tgaattttta atttcattta gtagcatgtc attttctgaa ttatctttac ctaattgcaa
    21961 atcctcaatt tcagttgctt ttatttctac tttgttttgt tcgttattct cttccacatt
    22021 attaggtacc acatctttat tactagctaa tattttgttt acacagaata taatttctct
    22081 ttttaattct cttttcgcat ctgcttgatt tttaagaaat aaatctccac attctttctt
    22141 ttctaaactt atttttctaa gagatatatt aagaaggtct ttatataggt ttaaaacctt
    22201 ataatccttt tctaaacgtc ttcttctctc tgatagttcc attttatcat aagctaaaaa
    22261 actatttaaa tcattggctt ttagagagtc taatccctct ttcatagcct tatttacata
    22321 ctttctacat tttgctgtag ttaactctaa atctttttct aattcatcta tttcaggtat
    22381 gtacttatct actatccatt tgccaataag tggttctgta tttcttgcta atgtcttata
    22441 aaacaatata ttaaatttta aaagctctgc atattttgct tcattaaatt ctactttttc
    22501 aaatttcttt tccaaaacac catctagctt ttcatttatt ttagactctt gttttaaaaa
    22561 acttgctgtg acatttaaaa cagtgtttct tattatatta ttttcactca aaaccttcac
    22621 tccttaaatc aattatttta ttgtaatatt tacattgtgc aaagttgcta atttgattat
    22681 gatatctaaa taattattta ctaaatttgc tacatcttat atcatatcat attatttaca
    22741 tcattttact ttttcttttt atatctatct tatattatac catttttaat atctgtacta
    22801 acttatatta caatattcct caaaatttta aaagtagatt agacaaattt acaaataaaa
    22861 aaacatcagt ttaaactgat gtttttgact atcgaaataa ttaagcaagt ctataagcgg
    22921 agttctgtat tagataatca tctttctagg cctattgtca ccaataggct caagcgacct
    22981 acctaccgga gagtacgagc agcactcttt attcctatct tggtcttgct ccaggtgggg
    23041 tttacatagc caactagtcg cctagctgct ggtgagctct tacctcgcct ttccatcctt
    23101 accacaatag tggcggttta tttctgttgc actatcctta ggatcgctcc cactaggcgt
    23161 tacctagcac cctgctctat ggagctccga ctttcctcat gtaagattac atgcgattat
    23221 ctggcttact taatatttat tttacttagt tatgttaaca caaattcttt tacatgtcaa
    23281 agtaatatta tactaaaaat ttaaatttat tcaaaaatag tgtaatatag atatataaac
    23341 tacatttaac tcgaggtgat ctattgaaaa aattatatag aatagtaatt aacattatac
    23401 tagtattagt aatactttac agtggattta atatatactc aaaacttacc aaatacaatc
    23461 atgatactaa aatatcctca gaacttcaaa aaaaagaata taaaaaagag gatttatcaa
    23521 agattaacag tgattttaaa ttttggcttt ctgttgagaa tacaaatata aattatccag
    23581 ttgtacaatc taaagataat tcttactatc ttgacaaaga tttttataaa aaagattcta
    23641 tttctggtac actgtttatg gattatagaa ataaatctat tgatgacaaa aatatcataa
    23701 tatatggaca taatatgaaa aataaaacta tgttcaacaa cctaaataag tttaaagatg
    23761 ctgacttctt taaaaaaaat aataaaataa aaataacctt gaatggaaaa gaatttttat
    23821 atgatgtttt ttctgcttat attgtagaat ctgattatga ttatctaaaa acaaatttta
    23881 acaatgaatc tgattatcaa aattacataa atgatattac ttctaaatca ttgtataaat
    23941 ctcctattaa ggtaaatagc aatgataaaa tagttacact ttctacatgt acctatgaat
    24001 ttgatgatgc cagaatggtg attcatggta gattgattta aaactcacag acacagttta
    24061 aaacaataat tttaaactgt gtcatctttt atacctataa tttgtaaatt agaccactaa
    24121 aagtatttaa tacaaaataa caaatacatc aaacaataaa aaacaataaa aaaagactat
    24181 gctaaaaaca tagtctttac tggagctggt gataggaatc gaacctacaa cctgctgatt
    24241 acaagtcagc tgctctaccg ttgagccaca ccagcacacc tattttattg taaattagca
    24301 aaactaattt ttaaaagtta
//
LOCUS       DAYAOY010000059        23896 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYAOY010000059 DAYAOY010000000
VERSION     DAYAOY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.059"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            71..144
                     /locus_tag="ACGI6B_002436"
     tRNA            71..144
                     /locus_tag="ACGI6B_002436"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Gly,seq:tcc)
     gene            153..269
                     /gene="rrf"
                     /locus_tag="ACGI6B_002437"
     rRNA            153..269
                     /gene="rrf"
                     /locus_tag="ACGI6B_002437"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            543..2561
                     /locus_tag="ACGI6B_002438"
     CDS             543..2561
                     /locus_tag="ACGI6B_002438"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9751875.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3066..5078
                     /locus_tag="ACGI6B_002439"
     CDS             3066..5078
                     /locus_tag="ACGI6B_002439"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9751876.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5320..7233
                     /locus_tag="ACGI6B_002440"
     CDS             5320..7233
                     /locus_tag="ACGI6B_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751877.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            7226..9445
                     /locus_tag="ACGI6B_002441"
     CDS             7226..9445
                     /locus_tag="ACGI6B_002441"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9751878.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9554..10384
                     /locus_tag="ACGI6B_002442"
     CDS             9554..10384
                     /locus_tag="ACGI6B_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFG9751879.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10374..13841
                     /gene="addB"
                     /locus_tag="ACGI6B_002443"
     CDS             10374..13841
                     /gene="addB"
                     /locus_tag="ACGI6B_002443"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HFG9751880.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13841..17668
                     /gene="addA"
                     /locus_tag="ACGI6B_002444"
     CDS             13841..17668
                     /gene="addA"
                     /locus_tag="ACGI6B_002444"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFG9751881.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17714..18925
                     /locus_tag="ACGI6B_002445"
     CDS             17714..18925
                     /locus_tag="ACGI6B_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFG9751882.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18912..22439
                     /locus_tag="ACGI6B_002446"
     CDS             18912..22439
                     /locus_tag="ACGI6B_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9751883.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22570..23823
                     /locus_tag="ACGI6B_002447"
     CDS             22570..23823
                     /locus_tag="ACGI6B_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFG9751884.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT        10257 a         2337 c         4212 g         7090 t
ORIGIN      
        1 taggatatat atgtagtttt tagagtgcta actctaaaaa attagtttta ctaatttata
       61 aaaatttaat gcgggtgtag ctcaatggta gagttctggc cttccaagcc agctgtgagg
      121 gttcgatccc cttcacccgc tccaaaagat tatgtggtta caatagcaga gaggatacac
      181 ctgttcccat tccgaacaca gaagttaagc tctctagcgc tgatggtact tggtgggaaa
      241 ctgcctggga gagtaggacg tagccacgta atcttttttt gttgtattaa atttaataaa
      301 cctacaattg ttatatgttt ttatttgtct tttatagatt tttttggcaa taaatgatat
      361 agtgaagata attaaatata ttcaatatat ttaatttttt tattttttgt ttttataatt
      421 tttttagaag tttatttttt atgtacttat ttataatttt gggtaaaaac actttgatat
      481 aatttcaaaa attaaaaggt taaaaaacaa agtagaaatt tatgttaaat aaggagatta
      541 atatgagaag aaatacaaaa ttattaacaa cagggattct ttcaatggca atcgtcgcac
      601 ctacaatggc atttgctact gaatctaatg ctatggaaaa taacgctgat ttaaatataa
      661 acttagagaa aaaaagtatc gttttaggta gtacctcaaa agtcagtgtt aagtttaaag
      721 aaaaaccaga tgcagatagc attacattaa agtataaatg ctatgatatg ccattggata
      781 caactttaaa ctataatcaa tcaactgagt catatgaagg aactatcaat tataataaag
      841 acccagaata tctaaatgtt tgggaactac aagggataac aataaacagc aaaaataatc
      901 ctaaaacttt aaacaaacaa gaactagaaa agatgggatt aaatttaaaa gactataatg
      961 taacgcagga atgtataatt gaagatataa cttctagaaa agatgtaaat aaatatttaa
     1021 gaaaaacttc tgcacctatt acagaactta caggaagtga tagatacgaa acagcagtta
     1081 aaataagtaa agaaggctgg aaaaatggtt cagataaggt agttataata aatggggatg
     1141 taagtataga tggcattata tcaactccac tagcaactac atataatgcg ccaatacttt
     1201 tggttgaaaa aaacaatgta cctaatagtg taaaatcaga attaaagcgt ttaaatccta
     1261 gagatgtaat tataattgga gatgagaatg ctatttctaa aactactgct aatcaaatta
     1321 aatcgactgt aaatgctagt caaacacgtc taaagggctc taatagatat gagacatctt
     1381 tattgatagc aaaggaaata gataaaaatc atgatgtgga aaaagtatac ataacaaatg
     1441 ctaatggcgg agaagtggat gcacttacta tagcagcaaa agcaggtcaa gacaagcaac
     1501 caattatatt aactgataaa aatagtatta cagacaacac atataaatgg ttaaagagtg
     1561 aggatttaca aaatgcttat tttataggtg gccctcaaat gatatcaaca aatgttataa
     1621 ataaggtaaa tgatataact aaagataatg ttactaataa tagagtatat ggagcagata
     1681 gacacgaaac aaatgcaaac gtaataaaaa aattctatac agatgatgag ttagaggctg
     1741 ttttagtagc taaatcagat gtacttgttg atgctttagc agcaggtcca ttggctgcga
     1801 acttaaaatc tccaatactt ataacaccaa agacatatgt atctgcatac cataaagaaa
     1861 acttagaagc taaatcggca aataaggtat ataaaatagg tggaggattg acttctaagg
     1921 taatgagctc tatagcatca tcattatcta aacacaatac aactccaaca gaaccaggaa
     1981 atagtggggg caagacagtt atgattgacc caggacatgg tggttcggac actggaacaa
     2041 caggtaaacc attagggggt attagagaaa aagactatac attaaatact tcacttgcga
     2101 caactgaata tttacgttca aagggattca atgtaataat gacaagagac acagataaga
     2161 ctttatctct tggaaataga actgctttat ctaactcttt gagaccagat ttatttacga
     2221 gtatacacta taatgcatct gatacaactg ggaatggtgt agaagtattt tataagctta
     2281 aagataaaga tggagggact actaaaactg tagctacaaa tatattaaat agaatcttag
     2341 agaaatttaa tctaaaaaat agaggtgcaa aaacaagaac actatcaact gaccctacaa
     2401 aagattattt atatgtttta agaaataatg atatgccagc tgtacttgta gaatgtgcat
     2461 ttttggataa tgagaaagat atgagtttac ttaatacatc aaataaagta aaagaaatgg
     2521 gtacacaaat aggtaaaggc atagaagatt cattaaaata gtctacaatg agagcataga
     2581 aaaatttaaa ataaattaat ctgcaatttt aaatttaaac taaagctata agataagaat
     2641 gtaattataa tagtaataaa gactgtatat acattgtgca tataagtgcg aaatgaggta
     2701 tatacagtct ttttatgatt aattgtaaaa ggatttatat tatatgaaga atttacatat
     2761 cttaaggcta cagttaattg tcaagagttg gagtattcat ttgtaataca caataaaagc
     2821 ttaattacta tattttattg tgtgtttgtg tgttttttgt ggatttttat gtcaataaat
     2881 gatatagtat agataattaa atgtattaaa tatatttaat ttttttattt tttgagtttt
     2941 ataagtttat ttttatatat tacttatata ttatttatat tttgggttag atacactttg
     3001 acatattttc aaaagttaaa agattaaaaa taaagtagaa atttatgtta agtaaggaga
     3061 ttaatatgag aagaaataca aaattattaa caacagggat tctttcaatg gcaatcgtcg
     3121 cacctacaat ggcatttgct actgaatcta atgctatgga aaataacgct gatttaaata
     3181 taaacttaga gaaaaaaagt atcgttttag gtagcaaatc aaaagttagt gtcaaattta
     3241 aagaaaaacc agatgcagat agcattacat taaagtataa atgctatgac atgccattga
     3301 atacaactct aaattacaat caatcaactg gggcatatga aggaactatc aattataacc
     3361 aagacccaga atatctaaat gtttgggaac tacaagggat aacaataaac agcaaaaata
     3421 atcctaaaac tttaaacaga caagacctag aaaagctggg attaaattta aaagactata
     3481 atgtaacaca ggaatgtata attgaagata taacttctag aaaagatgta aataaatatt
     3541 tgagaaaaac ttcttcacct attacagaac ttacaggaag tgatagatat gaaacagcag
     3601 ttaaaataag taaagagggc tggaaaaatg gttcagataa ggtagttata ataaatgggg
     3661 atgtaagtat agatggcatt atatcaactc cactggcaac cacatataat gcaccaatac
     3721 ttttggttga aaaaaacaat gtacctaata gtgtaaaatc agaattaaag cgcctaaacc
     3781 ctaaagatat aattataatt ggagatgaga atgctatttc taaaactact gctaatcaaa
     3841 ttaaatcaac tgtaaatgct agtcaaacac gtttaaatgg ttctaataga tatgagacat
     3901 ctttattgat agcaaaggaa atagataaaa atcatgatgt ggaaaaagta tacataacaa
     3961 atgctaatgg cggagaagtg gatgcactta ctatagcagc aaaagcaggt caagacaagc
     4021 aaccaattat attaactgat aaagatagta ttacagacaa tacatataaa tggttaaaga
     4081 gtgaggattt acaaaatgct tattttatag gtggtcctca aatgatatca acaaatgtta
     4141 taaataaggt aaatggaata actaaagata gtgttactaa taatagagta tacggagcag
     4201 atagacacga aacaaatgca aacgtaataa aaaaattcta tacagatgat gagttagagg
     4261 ctgttttagt agctaaatca gatgtacttg ttgatgcttt agcagcaggt ccattggctg
     4321 cgaacctaaa gtctccaata cttataacac caaagacgta tgtatctgca taccataaag
     4381 ataatttaga agctaaatca gctaataagg tatacaaaat aggaggagga ttgacttcta
     4441 aggtaatgag ctctatagca tcatcattat ctaaacacaa tacgactcca acagaaccag
     4501 gaaatagtgg gggcaagaca gttatgattg acccagggca tggtggttca gcacctggaa
     4561 attcatctgg aggaatgatt gaaaaagatt acaatttaaa tacttcactt gcaacaactg
     4621 aatatttacg ttcaaaggga ttcaatgtaa taatgacaag agacacagat aagactttat
     4681 ctcttggaaa tagaactgct ctatctaatt cattgaaacc agatttattt acaagtatac
     4741 attataatgg ctcaactaat aaacaaggtc atggtgtaga agtattttat aagcttaaag
     4801 ataaaaatgg agggactact aaaactgtag ctaccaatat attaaataga attttagaga
     4861 aatttaaact tacaaataga ggtataaaaa caagagtact tcctagtgat tctacaaaag
     4921 attatttata cgttttaaga agtaatgata tgccagctgt acttgtagaa tgtgcatttt
     4981 tggataatga aaatgatatg agtttaataa actcatctgc aaaagtaaaa gaaatgggta
     5041 cacaaatagg taaaggaata gaagattcat taaaataaat ggaaataaaa atatttaaaa
     5101 acttaatgta aatttatagt tgtaaattta tactaaagtc aaaaaatgaa atataagtgt
     5161 atcaatagta agactgtatg tacatttgca cgtgtgaatg caagtatata tacagtcttt
     5221 ttttgtagtt aaatgcaata atttgaatgt gttttgtaaa atacacaata tgatataatt
     5281 agataatata cacttaattt aattgaggaa ggtgattata tggaatattt atttgaaaaa
     5341 agtagtttaa aagaatatgg aaatgaagct ttagataaat atagagaatt attagataaa
     5401 actggtaata attcaaggtg tattcttttg ttagtaccaa acaataacac aaagatacga
     5461 tatgaaaggt ctctaagatt aaattatagt gaagaactga aaataacaac atatataagt
     5521 tttgtgaaga aagaattagt taaatactgg ccactaataa tagaaaaatg tgatgaaata
     5581 agtacaaagg ttgtatctcc aacatttata tcaaatagtt taagtgaata tttaatagtg
     5641 caaaaagtta aggaaaaaag gatacaagaa ggatattttg aagatattac aggaaccaat
     5701 aaatccatag caaatagtat aatgactaac ataaataagt ctgcatttaa tttaattgat
     5761 tttgatttta ttggagaaaa aatatattca tcaaagaaaa ataaagatag tatatataga
     5821 ttttcataca ctcaaatgga tgaaattata tcatattata taaattcctt actatcaaat
     5881 tcaattttag ataatgcatt aagcatatat ttatacaatc aatacctact taatgatgat
     5941 ttttatttaa aaaacttatt tacagaagtg agatatataa ttgtggatag ccttgaaagc
     6001 tcaagtaatg cagaagttga ttttataaca gaagcattgg ataatacatt acaaagttat
     6061 atatattttg attatagtag ggattattct gtatttaata atgtggatat agactatata
     6121 aatgagaaaa taatatcaaa aattaagcct aaagaagaaa atattaaaaa gaaggagatt
     6181 agaatagaag atttatactt attacctgca aatatagaat taaatcaatc aagtcaatta
     6241 tataatgaaa tgttagattt aataagtgaa aaagttatat ctcttataga agaagatgta
     6301 tctccaagag atatagcaat tatatctcct ataaacaata gtatattgga gcatcaaatt
     6361 agagattctt tgatagaaaa aaatatagat gtttttagta ctaagaagga taaaaaagct
     6421 gtggattatc catatgggaa tgcattggta gtggctacat gtatatttta tggatattta
     6481 gattttataa aagatgatga atacttaagc tttttagaaa ctcttttaga aataaatagg
     6541 ataaaagcat ttaagatttt taatgaaaca agacatttaa gtattgataa tatagatgaa
     6601 catagtcagt atcgtgagat tttacagtat attgaagaaa aaaagaagag tgatataaaa
     6661 atacatgaat ttttaactca attttacata gataaaatgt taaatttaaa agaaggtaaa
     6721 aagaatgttg ggttatgtaa aaagataata gctgaaagtg agtcttttag tgaaagttta
     6781 agtctacttg ggatgaaaaa agaaaagata tttatagaag ctttaaaaag tacaataaac
     6841 gattattact ctgttgtcga tatagaagag ttgaaatcca aagacaaaat agtaataact
     6901 actccttatt catatatatc atcaaatata gatagaagta tacaaatatg ggtagatata
     6961 ggaagtaatg cttggaatat gaaaatagaa aaagatataa gtaatttaat tgtacttaga
     7021 aaatcttttg aagaaaaaaa gatttataca aatgaaatgg aagaatatta taaaaaatac
     7081 tatctataca atacagtata taatttgtta ttaaatgcaa agaaaatcta tgcatttaaa
     7141 agtgaatatg cgattaatgg ttatatccaa gagagtatat tatatagtat acttttaaaa
     7201 atctcacata aaggagataa aaattatgat taatataaat tatagagaag accagatacc
     7261 aataataaat tacgataatg gtactatggc agtaccagca gtaccaggtg ctggaaaaac
     7321 atttataata acaaatttag tagctaaact attacttgag cagaaacata agggtggtaa
     7381 aatattaatt cttacatata tgaatagtgc tgtaaataat tttaaaggta gaataagaaa
     7441 aattcttgaa gagaataaaa tagatgatac taattcgtat gaagtaatga ctatacacag
     7501 tttggctgta aaaataataa aggaaaagcc agaagttgta atgttaagtg aggattttaa
     7561 catagctgac gatttgcaaa aaagtattat attaaatgaa tgtataagta gatttagatt
     7621 tgaaggtgga gaaagagctt ttagatggtt tttaaaggag caaaaagatg aacgctggaa
     7681 agaaataact cttgaagcct gggaaagagg tttttttgaa tttgcaggaa atgcaataag
     7741 tgaacttaag tataagggaa tttctccaga taaccttgaa gaaatattgt caagaggaca
     7801 caaaggaatg cttaaaatta ttttaccaat atataaattg tatgatagaa aattaaagca
     7861 aaatggattg ctagattatg atgatatatt aattttagca gaaaaaactt taagcttaga
     7921 cgaagggctt agaaagaagt ttcaaactag atacaaatat atatttgaag atgagtgtca
     7981 ggactcaaat gaaattcaag gtaatataat aaaaattata tctagcgaaa ataaaaacct
     8041 agttagagtt ggagatataa atcagagtat aacaggaaca ttttcatcat cagaccctaa
     8101 attttttaaa gaatttataa atagtgcaga tttctgttat aggatggata tgtcaaatcg
     8161 aagttcaaaa gatatacttg atttagcaaa cactcttgtc aagtatgtta caaaggaatt
     8221 tagacagaaa gaatgtagac atgctcttga agatatgcaa ataaaaactg tgccaaatgg
     8281 aatgggatat aaagaaaatc ctaatccaga acattataat ataaatgtaa agtggtatga
     8341 gagttggaaa attgaaatag agcaaactgc tagatatgtt aaaggtataa aaaataaata
     8401 tccagataaa agtataggta tattggttcc atttaatgaa caagtgacac aagttgctaa
     8461 agaacttaga gagaataatc taacttttga agaattagga ccaaattctt tgaataaaag
     8521 gaaggttata aacaacatag catatattat tgattttatg ttacattgtg atgatgtaga
     8581 aaagttaata gttgtactta ataaagtttt tatagatact gataatgaag aagaaataaa
     8641 gtatttttta gaagaattaa aaaactattc tactgaagaa attatttata attttgaatt
     8701 tagtaagtta gataatgatt taatagaatt taaagaagaa agtgacatat atttaagttt
     8761 taaaagagga atagatatta taaaacagat attagaatat ccaataataa ggttagacct
     8821 cttagttttg tttattggga aaaaacttaa tttagaaaaa gaagataaag ctgtactgga
     8881 ttatatatct ttttatataa agtatctagt atcagaaaat ataaatatgg atttagaaga
     8941 tgtatataat ttattgtttg atgtaaagaa cagggtgttt aatcatataa tagatgtggt
     9001 atatgagata aatggatatg aaccagagcc cggtagtata acactatgta actatcataa
     9061 atcaaagggg atggaatggg attgtgtgtt tttactaggt cttgttgaat ataatttccc
     9121 tgacaatatt aatcaaaagt ttcagagtga taaatggtat ctaaaagaaa aatataaaaa
     9181 ccctatggca attattaaat cagaagttga agctatatta aaaggaagta tttcaacaga
     9241 ttatgctcat aaaacgaaga ttgattctat aaatgaaaag attagacttt tatatgtagg
     9301 gattactaga gcaaaggaaa tgttagtact ttcaggaagt gcttatagag atgaaagtga
     9361 tatagggaat aagagaaaag agcaaaaacc atgtatatat ttaagcagac ttaatcaaca
     9421 tattattgaa aaaaggtcaa attaatatat aataagttat taaaagattg cttgaaatat
     9481 atctaaaagg ggataaaaca atatgtacag taagtaacta ctaaaaaata aagttaagag
     9541 agaagtgata tgagtgaacg ataaattaaa atatttttct tatagtcaaa attcaattaa
     9601 cacatataaa tcatgtccat tgaaatttaa atataaatat attgatagaa taaattggaa
     9661 aaatgatgat gttggtagta gagagtatta tgaaacttta aaaacaggaa gagattttca
     9721 tttgatttgt gaaagatatt ttagcaatat acctcttgga atctatttta atgaggatga
     9781 taaaaatagt aaaaagtttt taaagtggat ggaaaatata aaaaaagttg ttcctattga
     9841 aaaaggaaaa acgtatttgc cagaatatga agtaaggatg acattaaatg gggatataat
     9901 acaggcaaag tatgatttag ttgtaattgg aaataacaat atagaaatat gggattggaa
     9961 aaccgagtct aaaaagattg actataaaca cgtagaaaat agaatacaaa ctattgttta
    10021 tatgttttta gcaaaagaag taatacctaa agttttaaaa atggacataa atgttaaaga
    10081 tataaatatg agatattatc aacctgaatt tgatgactta ccaattacca ttttatatga
    10141 tgaagaaaaa catgaagcaa atagaaataa aattcaaaat tatataagta tgataaaaaa
    10201 cactaattat gaagaacata catacgaaga caatctatac aatgatatag aaaaggtata
    10261 taataaagaa gaacgtatgt gctatagaaa taaaaagcat tgcaagtatt gtgaatttaa
    10321 taagttatgt aatggcaaag aaatagatta tagtattttg gaggcggaaa tttatgggac
    10381 ttaggtttgt attaggtaga agtggaagtg gaaaatccac ctatatacta gatgaaatta
    10441 aaaaagaagc tcaaaaaaat gaaacgacat caattatact tttagttcca gaacagtaca
    10501 catttgaggc tgaaaataga gtaagtaaac tatttttagg aaaagaaaaa gataagtatc
    10561 taagagttag ggtacttagc tttaagacac taagtaatat agttttttct caagtaggag
    10621 gattgacaga tgtaaatata aattctagtg gaaaagcaat gatggtatat agagctattg
    10681 aagatgtaag tgaagaatta aatgtgtttt caaaatcaaa atcgcagtct ggttttgtaa
    10741 gttcaattac agatatgata tcagaaatga aacaatacaa tataagccct gaaatgttag
    10801 aaaatatatc tggagaattg gataatgaaa ctttaagttt aaaattaaaa gatataagta
    10861 aaatatataa ttcttttgaa gggaaattgc atgaaaatta tgtagatgct caagatatgt
    10921 taacttcatt ggcaagtaaa attgaattaa gcagttattt agatggagct tgtgtttata
    10981 tagatgaatt tacaggtttt acaccaaatc agtacaatgt cataaagagt atattaaata
    11041 agtctaaaag tgttaatatc tctttgactg tagatgatat caactatatt gggtacagta
    11101 aatctgatat gttttctaga acgaaattta cttattcaaa attgactcaa ttgtgcaacg
    11161 aagaaggcat aaaaatattg ccacaagtaa atttaaacac aggtgttata aaaagatttg
    11221 aaaaagttaa ggaattacag cacttagaaa gattttataa tgcttatcca tataaaatat
    11281 attcaaatcc aactgaaaat ataaagataa aagagtttaa caatttatat tcagaggtgg
    11341 aagagatagc cagagaaatt gtacatttag ttagagataa gaatgtcaga tatagagata
    11401 taaccattgc tacaagagat ttaaatagat atgatttttt agtacactct atatttaatg
    11461 aatataatat accaaacttt atagataaga agagagaggc aaaaagtaat ccaatagtta
    11521 ttttaattat ttcagcttta gaaatgaaga atagacgata tgggtatgaa actatgttta
    11581 ggtatttaaa aagtggactt ataggaattg ataatgatga tataaattta ctagaaaatt
    11641 atgtgttggc taatgggatt aaaggtaaaa aatggtttga tgaaaaatgg gattatagaa
    11701 taacccaaag tctttcaggt caagaaagtg aatttgaact ggaattaaaa gagaaaataa
    11761 atgaaataaa aaatagagta ttagagccta tagtaatttt acaagaaaaa ttaagaggaa
    11821 aaaatagagt aaaagaaatc tgtagatata tatatgaatt tttattagat ataaatatgc
    11881 cagaaactat agaaagttta atagtaaatt ttaaggataa aggggaactt gatgttgcaa
    11941 atcaatactc acaagtttgg gacatagtag tggacatatt agaccaaatg gtggaactta
    12001 tgggtgatga aataatttct ttagaaaaat ttataaaatt aataacctta ggttttgatg
    12061 aatatgaatt aggactggtt cctcctagta tagaccaggt acttgtaagt agtgtggata
    12121 gaatgaaaaa tccagacacg aaatatttat acctagttgg aactactgat ggtgtgtttc
    12181 cgctgattac aaaggatagt ggaatattga gtgataatga tagggaaagt cttggaaaca
    12241 aaggaatcga agttgatatt gatagtaaga ctaggagttt tgaagaacag tttcttgtat
    12301 ataaagctct tacttcgaca tctaagaatt taaccataac atatccaata tcagaccatg
    12361 aaggtaaaac gcttagacca tcaataatta tatcaagact taagaagata tttccaaata
    12421 tagaaaataa aagttattta gtggaagaaa ataaaagcac tgataaagat atacttaaaa
    12481 aaataacggt taaatcccct acatttaatg aacttataaa cgtaataaaa aattatgaca
    12541 gtgatggtta caatacagaa gagattaata gtatttggtt agatatatat aggtactatt
    12601 taaaagatga aatctatagt tctataacaa aaaaagttat aaaaggtctt agttatacta
    12661 atcaagttca taagattgaa gagaaaaaaa taaggtcatt gtataaaagt aattctctta
    12721 gcatatctag gcttgaaaaa tatgctgaat gtccatttgc ttactttata caatatggtc
    12781 taaaagcaaa aaagcgtaaa gagtattcat ttacaccacc agatttagga acttttattc
    12841 ataatatttt agataggttt tcaaaagaac ttttacaaga taatttaact tggagagata
    12901 ttgatgaaaa atatatagaa ttaaagatag gtataatagt tgatgaaata attttaaaga
    12961 taccaggata tatattaaat agttctgaga ggtataaata ccttgcatat agattaaaga
    13021 atatgttgac aactgctata actataatta gtcagcaaat aaaacaaggt tcatttgaac
    13081 caatagatta tgaagttaaa tttggcaaca atggaaaata tccacctata aaaatggtgt
    13141 tagaaaatgg acaagaagta agtttaattg gacaaataga tagagttgat gaatttgaag
    13201 aaggagaaaa taaatatata agaataatag attataagtc aggcaataag tccataagtc
    13261 taacagaaat atattatggt ctacaattac aattacttgt gtatttagat gcaatccttg
    13321 aaagtgcaaa ggatgaagat ataaatataa atccagctgc aatactatat tgtagaataa
    13381 ataatccaat agcaaaattt aatgaagata aagatgatga agaaatacaa gaagcaatat
    13441 taaaagaact tagaatgaag gggcttgtag taaaagactc tcacatagtt aaagagatgg
    13501 ataaatcatt aatagatgga gaaaggaaaa actctttagt aataccagta ggtcttacta
    13561 aggatggaaa tgtaggtaaa agtacttcag caataagcta tgaggatttc aaactgctta
    13621 gaaaatatgt aaggcatgct ataaaggatt tatgtgaaga aatgttaagt ggagaaatac
    13681 gtatagctcc atataaacat aaagatggaa catcatgtga cttttgtgat tactctgcta
    13741 tatgtcagtt tgattcaact ataaaagata acaagtataa aaatttaaat aataagagta
    13801 atgaagagat aataaaaatg atgaaaggag atgttaatta gtgagttctc ctaaatggac
    13861 aaaagagcaa ttagaagtta tagaaagtag agaatgcaat ttattggttg ctgctgctgc
    13921 tggttcagga aaaacagccg ttctagttga gcgtataatt caaatgataa caagtagaga
    13981 aaatccaatt gatatagata agttattagt tgttacattt actaatgctg ctgcgtcaga
    14041 aatgagagag aggatagggg atgctatagg aaaagcctta gatgaaaacc cagaaaataa
    14101 acatttacaa aaccaacttg ttttattaaa taaatctagt attacaacaa tacactcatt
    14161 ctgtttggat gttataaaat caaactttca tagaataaac ttagacccta atttcagaat
    14221 aggagaccaa actgaatgtg ctattttgaa acaagaagca atagaggaag tatttgaaga
    14281 tttatatgaa gaaagagatg aggggttttt aaatttagtt gaaagttatg cagaaagagg
    14341 aggagataaa gaagtacaag atattatctt gggaatatat tcatttgcaa tggcttctcc
    14401 tgaacctaaa aaatggttga ttgactcagc agaacgattt aatattgatg aaaattttga
    14461 tttttcacag tctatttggg ctagagctat attagacact gtaaaaatag agattaatgg
    14521 tctttgtcta aatatggaaa gggcattaaa agaagtggaa tcaatagaag aattagaaac
    14581 ttttgcagaa aagttatccg ttgaatataa aaaaattgct gacatttcac aagcatgtaa
    14641 taaatcttgg gatgaggcat ataaaaagat ggcaagtatg tcctttgaaa attatgtaaa
    14701 aggagtaaaa agaatttcaa aagatgctcc ttcgtatata aaagaatcaa aagaaaaggc
    14761 aaaaactata agagataaga ctaagaagtc tttagaaagt atagtaagtg ctacattcaa
    14821 taaagataat gattctatta gagaagaaat aaaatatctt tataacatag ttaaaccaat
    14881 atctagtgtt gtactgagat ttgaagagga atatagcaat aagaaaagag aaaaaggaat
    14941 aatagatttt aacgatatag agcattttgc attaaatata cttacagatg tagatgaaaa
    15001 aggtaatatt gtaccttctg atatagcagt tggatgtaga aacaagtttt atgaaatatt
    15061 tatagatgaa tatcaggata gtaatctagt gcaggaagta ctgcttaaag ctgttgcaaa
    15121 tacagaaact ccaaatagat ttatggttgg tgatgtaaaa cagagtatat atagatttag
    15181 acaagcaaag ccagaattat ttttgcaaaa atataataac tataatgata aaaagggaag
    15241 ctcccataga aaaataatgc tttacaaaaa ctttagaagt agagaagaag ttgtagatgc
    15301 tgtaaattat atatttgaaa atataatgaa tgagaatata ggagaaattg aatatacaga
    15361 aaaagaaaga ctaaatttag gtgcaaattt taatgtggat acagacgaga aatctattat
    15421 tggtggagca acggagatac acctaataca aaaagataat aaacttgatg atgatattat
    15481 aaatgataaa gacgatagaa ttaataacaa agaaaatgaa atagaggaag aagaaaaatt
    15541 agataatata caacttgagg ctagaatggt tggaaacatt atcaaagatt taatgaaagt
    15601 caatgaagat ggaaaaattc aaaaggtcta tgataaggga atagatggat atagacctgt
    15661 ggaatttaga gatatagtta ttcttttaag agctacatct gcatgggctc cagtgtttgc
    15721 agatgaattg atgaatatgg atataccaac gtatgctgat gttggagtgg ggtattttga
    15781 tacgattgaa ataaaaacaa tattgtcatt acttcaaata atagataatc caatgcaaga
    15841 tatacctctt atatctgtat taaaatcacc tatatttgga tttacaccag aggatttgat
    15901 tgatattaga gttcagagta aagataaaat tttttatgag gttttaaaaa gtacagcaga
    15961 gtatgatgga tttacagatt ctcaaaatga aaatgagtct gagtttatac caagtgaaga
    16021 gtgcataaac aaaagtaaag attttttaat taagctaaag gaatttaaag aaaagtcaat
    16081 gtacatgagt acagatgaat ttatatggta cttgtacaca aggactggat actatgccta
    16141 tgttggagca ttgccaggtg gaagtcaaag acaagctaat ttaaaagtat tgtttgagag
    16201 agcaaaacaa tttgaagaaa ccagtcttaa aggaatattc aactttgtaa attttataga
    16261 gaaattaaaa aaatctagtt ctgatatggg aagtgcaaaa acacttggtg agaatgcaaa
    16321 tgttgttaga ataatgagta ttcataaaag taaaggtcta gaatttccag tagtcatatg
    16381 ctctgcaatg ggtaaaaatt tcaatactca agattttaaa aagagtattt tatatcatca
    16441 taatttaggg tatggacctc aatttgtaga ctatgagaga aggatttctt ttccaagtat
    16501 agccaaagaa gcattaaaaa gcaaaataaa tatagaaaat ttatcagaag agatgagagt
    16561 tttatatgtt gcatttacaa gagctaaaga aaaattgatt ataactggtt ctactagaaa
    16621 tatacaagat agtataaaga gatggtcaaa tggaattgaa agtttagata caatatcaca
    16681 atacgagata ttaaaaggta aaaatttttt agattggata atgccttgtg tattaagaca
    16741 tagggattta tctaatctac tagaagaagt aggattagac gcagttttta atgttgaaca
    16801 taattcaaaa tggtatggaa agttgtggaa taagaatgat attttagtgg aaaagaagag
    16861 tgatgaagaa aaagaaagta ttgaagaaat tcttgaaaaa atagatgtaa ataatcctga
    16921 cagtgattat tacggtgaaa tagaagaaaa attaaactat atatatccat atgaatttag
    16981 tacaagaaaa cctgctacta tatctgttac agaaataaaa aagatacaaa ataattatga
    17041 agaagagtta ataaatacta tatttgaaca aaaagtaata ttaaaaaagc ctctatttat
    17101 tcaaaatgaa gaagaaagag aaaaaataag tggaacagaa agaggtacaa tagtccattt
    17161 agttatggag gttttagact taaaaaatgt aagtagtgta aatgatataa aatcacaaat
    17221 aagaggtttt gtttcaaaag gtataataac agaaaaacaa gctagtatag taaatccata
    17281 taaaatatat aaattttttg cctctaacat aggaaaaaga atgttgaatg ctgaaattat
    17341 aaatagagaa aaatcaattt atgcacaagt aaatatgaaa gacatatata tttatgaaaa
    17401 gcttataaac aatgacgata aaaaacttta tgataatgaa agtgtcatgt taagaggtat
    17461 agttgatgct tattttgaag aagataatca aatagttttg gtggattata aaacagattt
    17521 tgtaaatgag gaaaatataa atcaaataat agagaaatat aaaaaacagt tggatttata
    17581 tgcagatatt attgaaactt taactggtaa gtcagtaaaa gagaaatgta tatatttatt
    17641 tggagttgat gaagctgttt gttattagtt tttatatata aattaaacct aaaagataga
    17701 ggaaggggaa aaaatgagat ttattcatac atcagattgg catctaggga agtccctaga
    17761 aggccattct agaattgagg aacaagcaaa gttttgtgag gaatttataa aaatagtaga
    17821 atcaaatgaa atagatatgg ttataattgc tggagatgta tatgatactt ctaatccccc
    17881 agcacaagca gaaaaactat tttatcagac tgtatcaaga cttgccaata atggtcagag
    17941 gtgtgtatta ataatttcag gaaatcatga taatccagag agactctctg caataacacc
    18001 tctagcacat gaacagggga tacttatata tggttatcca ttaagtgcta cgatagaagc
    18061 taagtataaa gggtttgaaa taacatatgc aactcaggga tgtacaaaac tgaatataaa
    18121 tggagaaaat attgtggttg ctacattacc ttatccaagt gaaaaaagat taaatgaagt
    18181 tttttcaagt gaagatgagt ttgagaaaca aaaaaattat tctgaaaagg taggagatat
    18241 atttagaagt ttagaagaaa attttagaag tgatactata aatatagccg tatcacatat
    18301 atttgtaata ggtggagaga gtactgaatc agaaagacct atacagttag gtggaagttt
    18361 tttagttgaa agaaaagatt tgccagaaaa ggctcaatat actgcactag gtcatttaca
    18421 taaacaacaa aaagcttccg aacgtttaaa tgcatactat tctggttctc cacttcaata
    18481 tagcaaggat gagagagcgt ataccaaagg ggcatacata gtggatataa aagctggtga
    18541 aaaaccaata attgaagatg tatattttaa taattacaaa cctatagaag tatttaaatg
    18601 taatggaata gaagaagcac tagatatatg tgagcaaaat caagatagag agatttggtc
    18661 atattttgaa ataaatactg atgagataat atcacagaat gaaattaaaa aaatgaagga
    18721 actattaaaa gacataatag aaataaaacc aataataaca tcttgttatg aacaagaaag
    18781 tgtagatata aaagaaaagt ctatggcaga gttgtttaga gagttctatt cttttagtaa
    18841 aggtgtagag ccaaaaggag aacttatgga tttatttcta gatataataa gtgaagaagg
    18901 tgaatcagca gatgagacca attagattag aacttacagg gcttaatagc tatattgata
    18961 aacaagttat agactttgaa aaattaattg aaagaggttt gtttgggata tttggaacta
    19021 ctggaagtgg aaaatctaca atcttagatg caataactat tgctatgtat ggaaatatat
    19081 ccagaaatac aaaagaatat ataaacagtg tgtgcgataa ggcaattata tcatacgagt
    19141 ttgagatagg aagcaaaaac actagaagaa gatacattgt agatagaacc attgctagaa
    19201 gcaaaacagg aacaaagacc tctaatgcta gattaataga agtattgaat gacaataccc
    19261 aaaatgtttt agctgataaa gttgtggaag taaatgaaaa agtagcacag gttgtaggat
    19321 taacagcaaa tgattttact aggtcagtag ttttaccaca agacaaattt aatgagtttc
    19381 taagattatc acgtgcagat agaagagaca tgttagagag aatttttaat cttgagaaat
    19441 atggaagaag tcttggagaa aaagtgaaaa agagaaaaaa tatacagtta caaaatttaa
    19501 aagatttgaa atcaaaatta agtcaatatg atggaattac agaggaagta tacaacaata
    19561 taaaccaaga attaatagag ttaaaaaatt tggaaaaaga taaggataat gctttagacc
    19621 tagctcaaaa atcttatgaa gaaagtaaaa cagtatatga agaacaatta aagttagaaa
    19681 aaaatgaact tagaaaaaat gagcttgatt taaaaaatag tgagataaaa gaaaagattg
    19741 ctcttgtaga aaatgatagt aacgctaaaa aggtagaccc atacataagt tcagttcaaa
    19801 atctagaaaa aaagatagat gaagatagct tcgcagtaag taatttagaa aagaaattag
    19861 caatattaaa tcaggagctt gaagttacaa aaaatagata tgaaaaaata aacaaaatta
    19921 agaatgaaga agtccctaaa ttgagtgaag aaaaaataag attgcaacaa gcaataaaat
    19981 tagaagaaga gttagtttta ttagatagag agttaaaaga gctaaaagaa agtggaatta
    20041 atttaaataa gactaaagtt gaattagaaa aagtaaagca ggtgtcagag tccagaaaag
    20101 atgcagttac taaatctata aaagaagttg aaggaaagat tgataaagta aatataagtg
    20161 cagaattaaa acaaaaaata tttctagcat atgaatacga gaaagattac aacaacgtat
    20221 tggaagaaaa aaatcaaaag ctaaataaac tagaagaaat actcaaagag acagaaaata
    20281 taaatttaaa agttagatat attgataaag ataagaatga tgtaaatcgt aatttagaaa
    20341 atttatcact tcacttggat gttcttctta aaaaatgtcc tggtaagagt gctgatttac
    20401 ttttaaaatc tgaatatgtt actgaattaa gaaataaagc aaataatact aaagaaaatg
    20461 aaattaagaa aagcagtata caagatgaat taaagataat acttgaaagt aagtttaata
    20521 ctgaaaggga agtaaattta ctaaatgaaa aacttgaaaa taatagaaag aacagagatg
    20581 acttagaaaa agaattagaa gaactaaagt atttaaattt agcctctgaa cttagacgtg
    20641 aattaaaaga aaatatgcct tgtccagttt gtgggtcaaa acatcatgaa aatcatgata
    20701 taactaagta tgatgaaaat atatcttttg tcaaagaaaa actggagaaa ttagaaaaag
    20761 aaaaaataag tataagaaac aatatagagg aattaaatgc taaagtaagt gggtatttat
    20821 ctatagaaaa aatgaagact aaagaacttg aagatgtaaa aggaaaacta ggggggattc
    20881 catctagtca attattaaaa aaacttgatg aagaacaaag aaaacttgca ttgttaaaat
    20941 ctaatattca ggagtgggag aaagaaaaag agagtactga aaataaagta acattagcta
    21001 aagaagaaaa aaataaaatt gaaaaagaag aattaaaaat aagagagagt ctgaataatt
    21061 ataagaaatt aacaagagat ttaaacatag aaatagaagg tttagaaaat aaatgtaaaa
    21121 aacttaaaca ggaataccta ggattaaaga cgattactaa agtctctaat ctattgtcaa
    21181 aagtaaaaga aataagagag aatgaaaaag aattagaatt actaagtact gggcattcaa
    21241 atttattaaa aaatagagac tcactagata ttaaaataag agaaaatgag agtaaactac
    21301 atgaaataga gatagaatta ataaaggcta gagaattata tgtagaaaag aaattgagta
    21361 gagataataa gtataaagaa gttattagca taacgaaagg tgatttagct aaaaatcttt
    21421 tacataatgt tgaagaaaat atatgtaaga tattagaaca agaagaatca agtaagaaaa
    21481 aattggaaga gcaaagatta gaatatgaaa aaaacattgc tgaaaagcac aatatagatg
    21541 gtagacttaa aactgctaag gaacaatata aagaccaaaa atcaacacta aataaactat
    21601 tagcagaaaa taaatttgaa agtatatatg cagtaaaaag agctttatta gatactgatg
    21661 cagtaaaaaa attgattgaa gaaatactag agcatgaaga agaacaaaag ttattatcat
    21721 ttaaaataaa atcatcaaaa gaaaaattaa atggaagaag cataaagaag gagtactttg
    21781 aacagttaaa agatgaaatt tataatttga aagtagaaat aggaaatata tccaaagata
    21841 ttggagctaa tcaaaatcag ttaataacat taaaagactc tttagataag ataaatgatt
    21901 ttaataaaca acttaaggta gttgaacaca atgtggactt attggaagaa ttagataaat
    21961 gtgtacaagg aaataaattt gtagaatatg tttctacaaa ccaattaaaa tatatagcgt
    22021 tggaagcatc aaaacgattg gatggaataa caaaaggaag atatgcactt gaaattgact
    22081 caacactaaa ctttgtaatg agagataact ttaatggagg agaaagaaga agcattgata
    22141 ctctatctgg tggagagaca ttcttgacat ctctatcatt agcacttgct ctttcatctc
    22201 aaatacaatt aaagggaagt gcaccattag aatttttctt tttagatgaa ggttttggtt
    22261 cattagatag tgaactttta gaaattgtta tagaatcatt ggaaagattg catagtaata
    22321 atctaagtgt gggtataata agtcatgtcg aggaacttaa aaatagagtt cctgttaagc
    22381 tattagtttc atctagtgag gctggaatag gttcaaaagt aaaaattgaa tatagttaga
    22441 aaatatatgt taattgaata tattatctaa tatttccaaa taatatgtaa ttgtgatata
    22501 ataaaaacta atattaaagt tatttataat taatattaat ataaaaacat gtaagtagga
    22561 gtgtggtaag tgttggaatc gcccaataat ttgattcaga ttattttttt aatagtatta
    22621 cttatagggt ctgcattttt ctcagcatct gaaacggctt taatgtcgtt gagtaaaatc
    22681 agaatcagat atatgcaaga tgaaggagtt aaaggagcta agttagtaag ttcattaata
    22741 gaaaatccaa ataagctttt aagttctata ttggttggaa ataacgtcgt aaatatagca
    22801 gcaacatcca tatcaacatc tttgtttata ggattaatgg gagaaaaagg agtggcactt
    22861 gctacagctg ttatgacagt attagtatta atatttggtg agattactcc aaaaacaata
    22921 gcagcaaata attcagaaaa ggtttctcta ttagtatcta aacctataaa agcaattata
    22981 tttatattaa gaccaatagt atggatattt aatataatta caaatattat atttaaatta
    23041 tttggtataa caaataaagg agctaaatca ttcataactg aagaagagct aaaaactatg
    23101 gtaaatgtaa gtcatgaaga aggcgtcctt gaaatggaag agagagaaat cataaataat
    23161 gtttttgaat ttggagatat gcaagctaaa aatgcaatgg tacaaagaat agatatggta
    23221 gctatagata tggaagatag ttatgatgag ataatacagg tatttaaaac tgaaaaatta
    23281 agcagaatgc ctgtgtatga agaaactata gatgatattg taggaatact taatataaaa
    23341 gatattatat ttttatctga tgaagaaata gagtcatttg atattaaaaa ttatatgaga
    23401 gaaccttttt tcacgtatga gttcaaaaag ataacacaac ttcttgaaga gatgaaactt
    23461 gaaaaaagtc agatggctat agttgtagat gagtatggtg gaacatctgg acttttaact
    23521 atagaagatt tagtagaagt cattgttgga gatatagagg atgaatatga tgaagaagag
    23581 gatgaaatac aagttataaa agaagatgag tatattgtag atggaagtac taaaattggt
    23641 gatgttaatg aacttattgg tgtaaacttg gaatctgagg agtttgattc cataggaggt
    23701 tttataattg gacacctaag cagattgcca gaagaaaatg aagtaattga ggttgataat
    23761 ataagatttt gtatagaaag tatagaaaaa aatagaatta aaaaaataag aatatataca
    23821 taatgtaata atgttcttaa atttattaga agtgtataaa tataaaagat atcttttata
    23881 tttatacact tctttt
//
LOCUS       DAYAOY010000060        23190 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYAOY010000060 DAYAOY010000000
VERSION     DAYAOY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.060"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            105..995
                     /locus_tag="ACGI6B_002448"
     CDS             105..995
                     /locus_tag="ACGI6B_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9751885.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1213..2499
                     /locus_tag="ACGI6B_002449"
     CDS             1213..2499
                     /locus_tag="ACGI6B_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9751886.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2588..3031)
                     /locus_tag="ACGI6B_002450"
     CDS             complement(2588..3031)
                     /locus_tag="ACGI6B_002450"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HFG9751887.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3055..3849)
                     /locus_tag="ACGI6B_002451"
     CDS             complement(3055..3849)
                     /locus_tag="ACGI6B_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HFG9751888.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(4414..4890)
                     /locus_tag="ACGI6B_002452"
     CDS             complement(4414..4890)
                     /locus_tag="ACGI6B_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFG9751889.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(4991..6160)
                     /locus_tag="ACGI6B_002453"
     CDS             complement(4991..6160)
                     /locus_tag="ACGI6B_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9751890.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            6323..7186
                     /locus_tag="ACGI6B_002454"
     CDS             6323..7186
                     /locus_tag="ACGI6B_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFG9751891.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            7257..7850
                     /locus_tag="ACGI6B_002455"
     CDS             7257..7850
                     /locus_tag="ACGI6B_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HFG9751892.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(8101..8736)
                     /locus_tag="ACGI6B_002456"
     CDS             complement(8101..8736)
                     /locus_tag="ACGI6B_002456"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFG9751893.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(8816..9547)
                     /locus_tag="ACGI6B_002457"
     CDS             complement(8816..9547)
                     /locus_tag="ACGI6B_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFG9751894.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            9878..12067
                     /locus_tag="ACGI6B_002458"
     CDS             9878..12067
                     /locus_tag="ACGI6B_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFG9751895.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            12479..14407
                     /locus_tag="ACGI6B_002459"
     CDS             12479..14407
                     /locus_tag="ACGI6B_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFG9751896.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            14394..14741
                     /locus_tag="ACGI6B_002460"
     CDS             14394..14741
                     /locus_tag="ACGI6B_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HFG9751897.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(14983..16902)
                     /locus_tag="ACGI6B_002461"
     CDS             complement(14983..16902)
                     /locus_tag="ACGI6B_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9751898.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(16928..17710)
                     /locus_tag="ACGI6B_002462"
     CDS             complement(16928..17710)
                     /locus_tag="ACGI6B_002462"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9751899.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(17793..19241)
                     /gene="ddlR"
                     /locus_tag="ACGI6B_002463"
     CDS             complement(17793..19241)
                     /gene="ddlR"
                     /locus_tag="ACGI6B_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HFG9751900.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(19265..20164)
                     /locus_tag="ACGI6B_002464"
     CDS             complement(19265..20164)
                     /locus_tag="ACGI6B_002464"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFG9751901.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSGSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            20422..21678
                     /locus_tag="ACGI6B_002465"
     CDS             20422..21678
                     /locus_tag="ACGI6B_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFG9751902.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            21736..22515
                     /locus_tag="ACGI6B_002466"
     CDS             21736..22515
                     /locus_tag="ACGI6B_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9751903.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(22619..>23190)
                     /locus_tag="ACGI6B_002467"
     CDS             complement(22619..>23190)
                     /locus_tag="ACGI6B_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HFG9751904.1"
                     /translation="HFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYKLKKHY
                     LDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSERWEFK
                     HSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMNDLKFS
                     AYTCNSVINYILDSILSNTIKSYHIANCG"
BASE COUNT         8439 a         2899 c         3534 g         8318 t
ORIGIN      
        1 ttaattatag tataaattgg aatatttaga taattattat ttaatgtata agctaagttt
       61 ttaattaata tggttttata catttatata gaaaagaggt acgtatgagt agcacaatca
      121 aatcaaatcg aataaaaggt ataatgttta taatagcatc agcgcttggc tttgcaatga
      181 tgtctgcatt cgtaaagcta gctggagatt taccaagttt tcaaaaagtg ttctttagaa
      241 atctggtatc agctataata gctttttggc ttatcataaa acataaagga agtctaactg
      301 gtaaaaaaga aaatttaaaa gtacttttat ataggtcaat atttggaact ctaggagtta
      361 tatttaacta ctatgcaatt gacaagttga tgctttcaga tgctaatatg ctaaataaaa
      421 tgagtccctt ccttgtagta atattttgtg caattgctct aaaagaaaaa ataaatgtaa
      481 agcagatagg agctattata attgcattta taggagcttt attcataata aaaccaacat
      541 ttagtgtaga aataattcca tatcttgggg gaattgcagg agctgtattt gcagcaatgg
      601 catatacttg tttaagagtt cttggagata gagaagatta ctatacaatt gtatttttct
      661 tttcagtttt ttcattagtt actgtagggc caattgcttt tgcagtatat gaaccaatga
      721 cccttatgca acttatatat ctattactag ggggagtatt tgcaagtcta ggtcaatttg
      781 gaataactct agcatataaa tatgcacctg ctaaggaaat ctctatattt gactatagta
      841 atataatctt ttcagcaatt ttaagtatat tcttattcaa tgtctatcca gatatgttaa
      901 gtgttgttgg atatttaata gtattttcgg cagcatttta tatgttttta tacaataaga
      961 agttagataa attagacaaa gaaaagaaga agtaaactag ataaaagtgt ttttataatt
     1021 tgtattgaat atttaagtct ttgtattaaa attaaagaaa atttaattaa aaatgtttgg
     1081 tatattaact tataacagca gttaaaattg aaaaattata actgctgttt cttttattaa
     1141 aaatatccaa atgtctgtaa aaatagacta taatagagta gtataaataa tataaagggg
     1201 ataaattaaa gtatggaaaa agtgaaagtt gctgtgattg gagcaggtaa tagaggaacg
     1261 tatgcatatg ctccatatat ttatgaaaat tctgatgtat gtgaaatagt tgctgtagca
     1321 gaacctaaaa aaggaagacg agaactattt acacaaaaat ataacctaga tagtaaaaat
     1381 gtatttgaaa ctttagaaga cttttttaaa catgacaaga tggcagatgc agtaataata
     1441 gctacaaatg atgatagaca ttatgatgta gcaaaactag ctcttgaaaa aggttatcat
     1501 gtactattag aaaagccaat gtctaatagt ttagatggtc tggtacatat agatgacttg
     1561 tgtgataagt ataaagataa gatttttatg atttgccatg tactgagata ttctccattt
     1621 tataataaat taaaagaaat tgtagaaagc aaaaaattag gagagctagt tagtatacaa
     1681 tataatgaaa atataggata ttggcatttt gcacatagct ttactagagg gaattggaga
     1741 aatagcaatg aaaccagccc tttaatttta gcaaagagtt gtcatgacat ggatattttg
     1801 ttatatttag taggaagtag atgcaaaaaa atatctgcat ttggaagctt aaagcatctt
     1861 aataaccaaa atgctagtgg agagatggca caaaattgtc ttcagtgttt agttgaaaaa
     1921 aaatgtccat attcagcaaa gagaatttat ttagaaaaag atagaagtat aaatagagct
     1981 gttcacataa atccaactga agagaatcta ttaaatatac ttaagacaag tccatatgga
     2041 agatgtgttt acagatgtga taataatgtt gtagacaata tggtaaatat actacagttt
     2101 gaaaatggag tgacggctac ttttaattta tgtgcattta ctaaagaaaa tggaagaact
     2161 ataaagttaa tgtttagcca tggtgaagtt ggtggggatt taaataaaaa tgagataaga
     2221 attaaagaat ttggaaaaaa cgaagaaata gttatgaatc catcaaatca acagaatatg
     2281 gtagaggaat atgatagaaa tcttatagct gaatttatta aattagtttc aaataaagaa
     2341 ctagaaaagg gtagagtagc agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca
     2401 gcagaatatt ctagggtcag tgatgaagta gtgtatatag aagaattttt tgatagtgct
     2461 aagcaaatga ctaaggagat agaagaaact atattttaat taataaaaat ttattaaata
     2521 aaataaagat agattaaata atagaaatac gaaaatatgt gttctattaa ggaatctatc
     2581 ttttttatta aggttttttc ttatccctat caaaaaaatc catacattct gataaaagat
     2641 aagaaagtaa agcgaataag atttctgtac ttaaaggaat cgttactgaa tccataaagg
     2701 tatcaattat gttcatagct agaaaagtta gtataaaatc aattataaat tcgcaaagtt
     2761 tatatattga gtatggtaat ttgtttacat atctgaatat atctaaaata gtagtgcata
     2821 gaaaatcaat tggagtggtt atacaaaaat atattaaaaa gaaaattaat acagctccaa
     2881 gagatttata ttcaaatcct aagaatgata agaagccaat tcctataacc gtgaaaatac
     2941 aaaaagttat accaaaaaaa acagcaaaaa ataaaaatac agaaataaat tcgcctaaag
     3001 gaatccttct tttattactt atcttaatca actccttaat ttatgatatg aaatttatat
     3061 aaataataac attcttaaaa attcataata gtcctctgtt tcaaatagta ctgtttgaga
     3121 atctattttt ttcatatttg gataaaaaga ggccttaaaa gctttataat gttctctaaa
     3181 ttttatttct atcttaaatt tatctggcaa ttttattaag tgcctagata aatctccaga
     3241 taaagctttt ttgacacctt tttcaatttt ttttagtgca aggtttggat ggattgaaat
     3301 tgaaccattt ccaattcctt ttgacacagg aacagtgact atatttggat ttagtttttt
     3361 tgcactctca caaagcattt catctccact taaaaatgct acaggaacat tataatatgc
     3421 agcaatataa gcatttatag taaattcaga tgctatttca tcatttattt taaaatagtc
     3481 ataatcaata tccatagtat gggaaagtgg attaccatta caagtagctc cagagtgata
     3541 gccggtgaat atacatgcat caaagctttc atcaattcca gccatcataa ctagtgggtc
     3601 tttggtccat cctctaagta ttttgacgtt ttcaggtaaa atatttggat taatatttct
     3661 accactgtca tgtgcatctt taacaagtat ttcatgtaca ccagattgag tagctccatc
     3721 acatgctgct gttatctctt tactcatttg tgttctatag tgttgcgaaa attcacattc
     3781 aagttttgtt tcatcccaat ttacaatacc acaagtacct tctatatcag cacttaaata
     3841 caatttcata atatataccc ccttatgttt attattgatg ataataacag ataacagaaa
     3901 atacataatc aataaagggc ataaaaatat tatgtcttac tattaattat tattaaatct
     3961 gtaactgaaa aataactaaa ttggtattaa aaaatataac actatgtgtc atttatttat
     4021 atagttttta tatctgatgt ttatataaaa ttgtaacata taagtggtta catttactag
     4081 tttaacatag ttaaaatagt taggtattat ttttaagtta aattgattat tataaaatcg
     4141 cagaataaaa gaaagattta atttattaaa atgcatgaaa tgttttttcc tcaacataat
     4201 gctaaattaa actcaaaaag aaataataga aaaatcttat gtagcttaga aagaatgtga
     4261 atgatttaag ggaaaaatag aaatttatta tataatatta ggctgtatta tataatacag
     4321 cctaatatta tatgtatatg tgacataaaa tcttggtaag tgtaaataaa ataaatgtgg
     4381 aaaacaagtt aatgaaatca agaaaaaagt aatttaaaat tcaatttcat cttcccaatt
     4441 aaattctcct gatttttcta cactcttagg ccagtgacta caccatttag gaatggacgg
     4501 attttcaacc ctatcaacgc ttggagtata tggctttaag tctataacag gactattatc
     4561 attagcatca atatatggta tttttattat accattttca tggtcgatat tgataatttg
     4621 tacagcagtc aatgctattg gattgggtct tataggtgac cttgttgcaa atattcctat
     4681 aacgtctggt ccacttttat aaggttttgg ggtttctaaa atacttctta gttcaggatt
     4741 atcaaaccca tcagcccacc aaaatacatt taaatgactg aaatcgtcga gttcatttaa
     4801 tgcaggtaca tactttttgt ctaatttaat aaagacctct tcgccttgaa tacatatttt
     4861 acctatagga tttattaata tttctttcat aatattacct ccaattattt aatttatcat
     4921 ttgtaatttt agggtataac atgacatcat gtgagggtca atataaaata ataaataaaa
     4981 atattaatag ttagaatgat aatacatttt ttacatggag catgacacca gtaataaggc
     5041 aatcttcgtc aatatcaaaa gtagaggtat gcaaagggga agtaatgttt ttttcacgat
     5101 ttgcacaacc aaggtggtaa aaagctcctt cacagtgtct tagaaaatac gaaaagtctt
     5161 cagccccaag agtgggcgag tctttaagga taatattttc ttctccaaac aattctacag
     5221 cattttgttt gacgtaatct actagatggt ttgaattgat aagagctgga taaccttcct
     5281 caatttcaac agaaatactt cctccaaatg ttttacaagt aaaatcgcaa atttctctaa
     5341 ttcgtttttt tgaaaactct ctagtttcag gagtcaaggt tctcaaagta ccccttataa
     5401 ccactttatc acaaactata ttttccttga ttccaccttc aatctttcct agagaaataa
     5461 ccacagaatc agttggattt gtgtttcgac taacgattgt ctgtagagct gtaataatat
     5521 gagcagatat aacaatagca tcaatacctt gatgtggata agctccatga cattttgaac
     5581 catgtacagt taattgaagt gtatcagtgg aagcatttag agtgttgtat tttaattcaa
     5641 ttagattttt gttgatattg gaatttacat gaagtccaaa tatatatttt acatttgggt
     5701 tttcaagaca tccatcttct atcataagtt gagccccacc aatagtttcc tcggcaggtt
     5761 gaaaaaagaa ttttacattt acatttaagt aatcctttaa tttaaatagt atattacaag
     5821 tacccagtaa aatagcagtg tgtgcatcat gaccacatgc atgcattttg ccaatattaa
     5881 tagatttata agaataatta agttcttctg ttataggtaa ggcatctata tcagctctta
     5941 tagcaactgt ttcaaagttt ggactcacgt taatgtatgc agttatccct gtatgattct
     6001 tatattcaat ataatttata cctatttcat ttaagtattt aataattttt ttcttagttt
     6061 gaaattcttc taatccaagt tctggagtct tatgtaaatc tcttcttata tttataaccc
     6121 aatcatttat ctcatgacaa agagttttta tattaagcat aaaacacatc ctaactatat
     6181 taaaaattta tttttcttca ttaattatat tattttatgt aaatcattat tatgtatcaa
     6241 gtacaaataa aatatcttat aattatatta gagatattta gtatggagaa gaatatatca
     6301 ttttataaga agaggtgttt aaatgttagg aataattgcg ccatctgggc cattaagaaa
     6361 tacaagtttg gaggaaataa agtttaattt agaaagttat ggttatgaag ttaagttttc
     6421 tgaaagttgt agtttaaatt ataaaggtta tttagctggt aatgatgata ttagagcaag
     6481 ggatatagaa gatatgtttt tagataaaga tgtagatata attatgtgct taagaggagg
     6541 ttatggaaca acaagaatat tggataaaat aaattatgat ataataaagc aaaatccaaa
     6601 accatttata ggtttttctg atataacagg tttaaattta gctttctata aaaattgtgg
     6661 tcttttacca tatcatggaa taatggcagc aaatgtaggt aaatgggata aatttaccta
     6721 taaatcattg gtaaatgctt tagaatttaa agatgaatta tatttagaaa atccaaaaga
     6781 agaaaagatt tatactgtat gtgaaggtaa agcagaggga ataataatgg gaggaaattt
     6841 atccttaata attgccacaa tgggaacaaa atatgaaata gatgcaaaag ataaaatttt
     6901 gtttattgag gaaattggag aacctcaata taaactggat agaatgttaa cacaactata
     6961 ttcttcagga aaacttgagg aatgtaatgg tataattttt ggagatttta aggattgtat
     7021 tgaagaaaat gatttgatgg aattactaat agaatttgca aataaggtca ataaaccttc
     7081 tatatataat cttcagtcag gacattgtat cccaatgata actattcctt tgggcagaat
     7141 gtgcgaatta gatgctacag aaaaaatcgt taaattaaaa aaatagtgtg tatataaata
     7201 taatagaaat atggtataat cttatacaag taaaaattta gctaggagga tgaaaaatgt
     7261 ctgatgatag aataatagac tttaacgaac taaagaacaa agttaaagat tctgatgtag
     7321 ataaatttga gcaatacata tataacttat atttttctgt tatggatgga aagatgtcta
     7381 tggctgagtt ttcaagaaaa atattcgatt atatgagaga taacaacata tctcaagaaa
     7441 aatttatgaa aatacaaaag caatttatgg agagatatgg tatggacact gaagaagtag
     7501 aaaaacaact tagaaacttt ggaatcgacc cttctacggc aggttttatg agtaataata
     7561 cttccagcaa agtatctaca gaagatttag agtcatttaa gaagagtgca ggattttatg
     7621 agaaatatgg agaaaaaatc caaccaaaga gttgtataac aacatttata aaaaatgact
     7681 taaatgatat taatgtaata atcgaccaag aaaagataat gctttgtagt gatagaaaaa
     7741 taaatcttat ggattcagaa ctaaatgagt ttttattaga atataagaat atgtttaata
     7801 aaaaaataaa ggtagtaatg tgcgaaacta cgaataagta tgattattaa aaaatgaatt
     7861 taaatactga agtagtatca attaattgta ctacttaaaa ataaaaatcg caatttatta
     7921 ttccttataa atttgtataa ggaatatcta aactgcgatt ttttatattg tgaagattag
     7981 taataataga tagtgtttta aattgtaatc ttctcactaa aactgtgttt ttctattaga
     8041 aattttatta aaaataaata tctgataatt gtattatatg ggatgtaaag tattagaagc
     8101 ttaacgaatc tcttttaata cattaactcc attttgaatc atactgttga aaaatactaa
     8161 gtttaaaatc tcagcagggt gaacgttagg aatatcataa gtatctacta aattcatagg
     8221 tacaactata tttttttcaa tattatgttg attaaaatat gattttagag ttattgcaaa
     8281 ttgatatata catatatctg tgcaatcgcc aactataatt atattatcta tattatcaaa
     8341 atccaaattt tctaatgcaa aaaaaccatt agtagaattt ttaggaagaa tttgtaggtt
     8401 ttctatcgac ttaagttcat caaccaattc actttctaca tcattttcca aacagtgaac
     8461 aggataactc aaaagttcta ttgatttagg agtatgagaa tcagtaaatg ctattactct
     8521 atttaatttg tttgatattt tttttgtaaa catttcaatt ggctttatta aagactctac
     8581 tctaggagag tataatgcac cttgtcttgc aaagccatta ttaacatcaa ttataaataa
     8641 agcagtttta ctcaaatcat aattttctat tttttctatt ggtagattat ctaaattact
     8701 ctttaaagct tcaagttcat ttaataagtt atccatagtt atacctccaa gataaattta
     8761 ataaaattgt acatattaat tatataaaag tcaaggagta caaactattg tataattagc
     8821 tgtattgttt tgagtgtaaa agatttgaag caatatctgg atagttagta aaaataccat
     8881 ctacattgag ttttagacag tgtagtatat catcctcttc atttacagta tatacattga
     8941 caccaagtag atttttgtga caaagttcaa ttagagattc atttactaaa actagaggtg
     9001 gatgaagata actattaccc agaaatttag caaaattgac tacattgttt ataggtaaat
     9061 aacacaataa tcctgttttt acactagggt ctaatttatg aaagtttttt aagctattgc
     9121 tgttgaaact agatataaga actctatttt tcatattata ctttaaaata agctctaaaa
     9181 cgcctttttc tatattggga tagtttattt tgtcagtttt tagttcgata tttaaagtaa
     9241 ggtctgtagg tgagattaga attaatactt cttctaaagt tggaatttta cattctttat
     9301 tgctttgata tccttcaaaa gatgagttta gagtttttaa ttttcttaaa gtaaagtctt
     9361 ttacaaaacc atgtccatta aaagttctat caaccttttc atcatgaata actacaattt
     9421 ttccatcctt agttctgtgg acatcaagtt ctattccata tatatccata tccaaacatt
     9481 ttttaaaagc tagaatagta ttttctggat actttccact aaacccccta tgagcataaa
     9541 tattcattta tatggttccc tcctataagt ataacttatc ttataaaaat ttaattaata
     9601 taatatatca tattccaaaa ggtattgtta tatattagtt aaataactat ttgtaattta
     9661 tatattatat aaatgtagaa aaataaaatc atatatgtgt tataatatat tttaaacatt
     9721 gtaaatatta cgaatttagt agtcataata agtgtaattt tatgcaagct catgttttta
     9781 gagcttgcat atgtattatt aatatattta tatattgtga aaataaaatt ccaaaagaat
     9841 tttttctata gacttagtag taagggggaa ttttgatatg tatgaagtta atttagaacg
     9901 agctatgaat tattggatgg atttgttaga tgattatacg aatatagcaa aaattccaaa
     9961 tgattttttt tataaagatt gttctgatga agtagtagat gaggttttta agatgaatat
    10021 tgagaaacat gaaaggttaa ttcaatattc taaagtgaac aatattagca tagactctat
    10081 catagaaact atatatggcc ttatacttca aaagtatata tatgaagatg atgtagtttt
    10141 tggtaaaaca atgaatataa ttccaataag agtaagagtt agacaaagtg aaaaatttat
    10201 agatgttgta aggaagctaa aaaggcagtg gagcaggtct ttagagtatg gatactattt
    10261 gttatcagat atagagaata aaaatcatct aaatactaag cttataaaca caatatttat
    10321 agctaaaagt tttgatttat catatgatta tgacatagaa aaaaaaggat atgatttatc
    10381 tgtaatagta tatactaaga gggcacttaa aataaaaata aagtataaac catctatata
    10441 taaaagagaa actattagaa gaattttaaa tcaatttgat tatataatag agcaaataat
    10501 agaagataat gatattttaa tagaagattt agaatttgta tgtaaggaag aagaaaaagt
    10561 attattggaa gactttaata ataatcaata tgaagtaccc tatgataaaa gttatgttga
    10621 tttatttagg gagcaagtta aggtaacacc tagtaatata gctgctaggg atgaaaatag
    10681 aaagttaaca tataaagaat tggatgaagt aactgataaa cttgcaggtt atttaaatca
    10741 tataggagtg gaaagtgaag acatagtagc tgtaatgtta ccaagagata ttaacataat
    10801 tattacagca atagggatta tgaaatcagg gggagcattt tttccaatag acacaagtaa
    10861 tccagaagaa agattgaatt accttcttga agatagtaat gcgaaagtag tgataactac
    10921 agatgaactc aaaagtaagg ttgtaaatga gaataccata gtactcgata taaatgatga
    10981 agaaatgttt aagtttggat atgaattaac tgaaaaaata acatcatcaa attgtgcata
    11041 tacgatatct acttcagggt caactggtag gccaaagacc atagctatag aacataagag
    11101 tttagtaaat atgtgttatt atagcgtaaa atctataagc gctacagaaa atgatatatg
    11161 tggtatatac ttaagtttca gttttgatgc tgtaatgaaa caactattcc cttatttgct
    11221 tgtaggtgca tcaatagata ttatgcctga agaagctaaa tttgatgaat atacagtaaa
    11281 tgaatactgt gaggaaaatg acataactat actagcattg ccaacagcat ttgcaaggtt
    11341 atttatacaa aattgcaata ataattcact tagagttgta cagactggag gagaaagatt
    11401 aaaaggatat agaaagcgta attatgaact atataatgag tatggaccaa ccgaatttac
    11461 tgttgtatcg acttcttttc atgtggacag agaatatgga aaaataccaa taggaaaacc
    11521 tatatttaat acttatgcat atgtattaga taaaaagaac aaactatgtc caattggagc
    11581 accaggagag ctatgtctat caggcattca aatatcaaga ggatatttaa ataaaaaagg
    11641 gctaacagag caagtatttg tagagaatcc ttacaaaact tgtgaacata ataagttaat
    11701 gtatagaact ggtgatttag taagatggtt agatgatgga aatttagatt atattggaag
    11761 gatggacaat caagtaaaaa ttgatgagtt taggatagag ctatatgaga ttgaaaatat
    11821 aataaataat attacagaaa ttaaaagtgt tgtttgtata tcgagaacaa atgatgatgg
    11881 agatatttat atatgtgcat attatgtaat tgatgaggaa gatagtggta agataaatga
    11941 aagaactatt agagaatatt taaatgagca tctacctcca tatatgattc caacaataat
    12001 aatgaggata gataagatac cagttacacc aatcggaaaa gtaaataaaa gagcattacc
    12061 agaataattt ttttattatg ataggtgtca taaagtgatt tcagacattt ctttatgaca
    12121 cttattttta taaaataatc aatttgcata aaaacaagaa ttttctgtat atatttacta
    12181 tggtgaaaat ttatttataa gaatgattat tgtatttttt atacttttcc gtgttttaaa
    12241 gcattttttt tacaaaatta attttacatt aaaaaatttt tttattttat atcttaatag
    12301 tttttagtag aattgaaaaa taaaaagaaa attagcataa aaatatctaa aagtagaaat
    12361 taataatata gggttaaatt ttagtagttt tgactcgcta tttgtaaaat aataaaaagt
    12421 taagttttat atgtatatat gttataatat atacaaattg aaaaggggtg aaaattttat
    12481 gaacaaaaaa ttacctaagg gtgcttatgg tgaggttagt ggaaaagatt acgttccata
    12541 tatcacagat aagtccagaa ccggtggaaa tgtagctgta ctgattatcg gtattatttt
    12601 agctgccatt tttgccgcat ctacaactta ctctggaatg aaagccggtc ttacagttgc
    12661 cgcaggtatt ccaggagcta taataggttc tgcttttgtt ggagcatttg ctcgttcaaa
    12721 gggtattctt ggtaaaaact taatccaagg tatgtcaagt ggtggagaat ctgttgcaag
    12781 tggatttatt ttcgtattac cagcagttat attaattgga agccaaatta cattcttaga
    12841 aggcttagct gttggagttg gtggagtttt atttggtata ggaattgctg ctatagttca
    12901 caactatcta atagttgaag aacatggtaa acttatgtac ccagaatcaa tggctatatc
    12961 tgagacactt gttgcttcag aggctggtgg agattcaatt aagtacatgg gaattggatt
    13021 tgtaataagt ggatttatta ctgtattaac tggttcattc ttaaatgtag caaacaatgt
    13081 aatgagtctt gtaggaagca aattctacaa atggaaattt gatattgaag taaatcctct
    13141 acttttagga attggattta tagtagggct tgaagtttct ttaacaatgt ttgcaggaag
    13201 tattttatca aactttggta tagcaccttt aataggatac tttacagata tggctaaaga
    13261 tggagctatg gtatggaata atcctgcaat gcctcttaac caaatggatg ttggagcaat
    13321 atctagtagt tatgtaaaat atataggtgc tggtatgatg ctttgtggag gtattattgg
    13381 tgcaataaaa cttataccaa ctataatcgc ttctataaaa gaaacactta aagctaaatc
    13441 aagtgctgga gaaggagaag aaggttcatc tatacaaatg atacttttat taggtggagt
    13501 agttattgga tttttagcag cattcttaat ttctggtaac attgttatgg caattattgg
    13561 agctatcata tctcttttat tatctttatt atttgtcata gtagctggtc gtttaacagg
    13621 aacaatcgga acatcaaatc ttccagtttc tggtatgact atagcttctc tagttattgt
    13681 aacattagtg tttgttataa tgggatggac tgatttagaa gctaataaat cattactttt
    13741 atttggttca tttatagttg ttgctattgc tatagctgga ggttatactc aatctcaaaa
    13801 agtaacttat attattggtg gtagcaaaaa cgaaatgcaa cgttacttta caattgcaag
    13861 tatagttggt gttatagttg ttgtaggagt aattttatta ctttctgacc aacttagagc
    13921 aactggtgat aatgtacaat ttgcattacc acaagcaaac ttaatgtcaa cattaacttc
    13981 tggtattatg tcaggtagtt taccttgggt tatgattatt gttggagtat ttatggcaat
    14041 agttctatat gcattaaatt taccaataat gactatagct ataggattct atttaccaat
    14101 tgcaactact tctataatat tagttggagc attaattcgt ttatttgttg aattagtatc
    14161 taaaactgaa aaagaaaaag aagttaaagt ttctaatggt ataagtttat cttctggtct
    14221 tgttgctggt ggttctatca ttggtttaat aggtataata ttacaagtaa ctggagttgt
    14281 aactccaaag gtaccaagtg gatttgctgc aacaaattca atggcaattg ctttattagt
    14341 agttttagtt gtgcttacag cattacctat tgttttatct aaagtaaaaa ataatgagca
    14401 agagtaatga tgttttagat attgtattta aaattactga tggacttgaa tatgaagtag
    14461 ctgaaaatcg tgttgttact atacttgaaa aacaggacca taaaattcaa aagtttttta
    14521 gaaagttaaa agctagaata cctaaataca aaaaaataga actagatgaa tattccagtt
    14581 ttgttttttt acaaattgat ggtcaaagga ctgttagaga aataggcaag aacctagaag
    14641 ctaaatatgg agaagaatct catccacttt atgaaaggtt gttagtattt ttaaatcata
    14701 ttgaagttaa ttgtcactat attgaaagac tggatatata gatataataa gaattattat
    14761 agatatatta gagcatataa attagaaggt aattatgaaa gaggctgtct caaaatagag
    14821 ataagcctct tttgtattgt tgaaatgatg aaaatatatt tgatttctca taaaaaagag
    14881 tgtctcataa aactaaaaag ttcattttga gacacttcct ttaaaatatt tataaattac
    14941 agatttaaag caaaacttat taaacttaag attattttaa tattacttat tattagctat
    15001 ttcatcagct agattattta aatattccca tctttcatat ttgtattcaa gttctttttc
    15061 tagtttagat ttctcatcta aaatttcttg gagtttagta aaatcagttg aaaattttgc
    15121 agtactttca tcaagtgttg caattttaga ttcgagtttt tctatatcag agtcaatatt
    15181 ttcaaactcc ctttgttcat tatatgagaa cttaagcttt ttatcatttt taggtttttc
    15241 tttttttggg gtagtagagt catctttttt aactgattcc cttttagatt cttcaaattc
    15301 aattccttga atctctctat aaataagata atcactgtaa ttacctgtat agatatgaat
    15361 ttttccatta ccttcatatg caaatatttt attacaaatt ctatctaaga aatatctatc
    15421 atgtgatact gtaactacta cacctttaaa ttcgtctagg tattcttcta atattttaag
    15481 cgtttcaata tctaaatcat ttgtaggttc atctaaaaga agcacattag gagctgtcat
    15541 aaggatttta agaagatgta gtcttcttct ttcaccacca gataatttac ctatcaaagt
    15601 atattgcata gtaccattaa aaaggaatct ttcacacatt tgagaagctg taatcctagt
    15661 accatctgaa gtttctatat agtcactagc ttcttttaca tagtctatag ccctcatatc
    15721 aatattcata tgagagtcgt cttgagaaaa acaacctatt ttaactgttt ctccaattga
    15781 aatatatcca ctatcaggct ctatttctcc attaagtata tttataaggg tagatttacc
    15841 cataccattt ttgcctatta taccaattct atctgtacga gttaaagtgt attctaaatt
    15901 atctataaca gtattttctt caaatgattt agatatattg tgtatttcta ttattttatt
    15961 tccaagtcta cttgatgcaa cagatatatc gagattttca tcaggtttaa aagaatctct
    16021 atttataagt tcatcaaatc tttgtaaacg agctttttgt ttagtgctac gagcctgagc
    16081 tcctcgtctt acccacgcaa gctctgttct tattaaattt tgtcttttat cttcaatact
    16141 tgcttctagc gaaagtcttt ccattttctt ttccaaaaat attgaataat tcccatcata
    16201 actgaataat ctacctttgt ccagttcaat aattctattt gtaacacggt ctaaaaagta
    16261 tctatcatga gttatcataa gcaatgaacc ttttctggaa ttaagatatt cctcaagcca
    16321 atctatagta tcattgtcta gatggtttgt aggctcatct agtatcaaaa actcacaagg
    16381 tgtaataagg gctgatgcca gagagactct ctttctttgc ccaccagaaa gttctgctat
    16441 tttttgatta aagtcattta taccaagctt tgtaagtact gacttagctt cactttctaa
    16501 atcccaaaga tttaaggcat ctattttttc gtgtactgat aataattttt tatttaattc
    16561 ctctgtaaag tttgaattaa gtttttctag gatttcttga tattcaccaa gtagctttaa
    16621 ttcttcagaa gttccattaa atacttgttc aagtacagtt gaattttcat tgtaagttgg
    16681 attttgaggt agatattcta tcctaatacc attagttttt gttatattac cagtctcact
    16741 ttcttcaact cctgcaataa ttttgagtaa ggtagattta ccagtaccat ttacaccaat
    16801 aaatcctatt ttttcccctt catttattcc aagagatata ttttctagta attttttttc
    16861 agaatagctt ttacttatat tttccaatgt cattaaattc atataattca cctttatttc
    16921 taatctatta tttttcaaaa tgttgataat ctgggttttt ccaatagcca ccccaactcc
    16981 aacctctgct tacaaaagca ttataacaat catcaccttt ttttatcatt ccaaattttg
    17041 tgctagacct atcagcataa actgtactta ctttaggtgt aacatcattt ccagaaactt
    17101 gaggattctg taatgggttt atatctatag cacgtccttt accatgattt gaaactacat
    17161 ttgtaccagc tatagttcta taacaaaatg atgaggtatt attatcagac atagattttt
    17221 catctacagc atcatagtca tctattaact taattttttc aattggatat tttttttcgt
    17281 ataattcctt aaaaatatcc actacttctg gagctagttc actgtttact atcatttctc
    17341 cttgatgagt tttttcatca aatccataat atgtaagttt taaataagat agactatcaa
    17401 agcttatagg ttcatcagta ggcatagatt taccaatcat tttatttttt atattatctg
    17461 gtattgaaga atatgaaaaa gtaggtttga tattattatc ttcctgcttt ttggtagagc
    17521 tttgattatt tttagaatca tctgttgtat tatttttatt ttgcatagtt tcttgactct
    17581 ttatattacc ttttttgttg ggattagaga acagtatctc atatatagat aataaagcaa
    17641 gaaataatac agttattaca atcgttaatt ttaatttatt aatttttctt cgttttgact
    17701 ttttactcat attatccccc agtattagat tcatataaac tatatatatt atacctcata
    17761 aaaataaact aggctaacaa tatttagtta acctagaaca tttcatttat agtttctcca
    17821 attattttta ttccaatttc aatgtcttca aaagacaacc tagaaaatcc gagccttaaa
    17881 gtatcaaaac cagagttatc tgtaaaaaat aagtctccag gcataaatat aacatttttt
    17941 tcataacatt tttctaataa aactctagta tctatatttt ttagttttat aaatatatgc
    18001 aaaccaccat cacccaagat atattctgtt tttatatatt tttttataca tttatatgca
    18061 aagttaaatt tgtctccata gaatttacgt atttttttaa tgtatttttc aaaacctcca
    18121 ttacttaaat aatcaaataa aattccttgg tctaaaaagg atacatgtat gtttctgcac
    18181 ctttttacac tttctagctt attgattact tttttatctg caaatatcca tccaattctc
    18241 attccaggaa ataaaatttt tgaaaaacta ccaatatata cgaccccatt attcatatta
    18301 tctagggaac agataggaaa tatatgagaa ctattgtaga gtaattcttc attaaatcca
    18361 tcttctataa ttgctatatt gtattttttc ataagattat agaatttgta cctattttca
    18421 ggagtcatta caattcctgt aggattgtga taagagggtg taatgtatgc aaactttatt
    18481 ttatttttat acttaactaa attgtcttct agcatattga agtccaaacc atcttcattt
    18541 atatctacac ctaagatatt gaggttatga gatttcataa tttttatagc agtattatga
    18601 gtaggatttt cacatattat gtagtctttt tcttgggtaa aagatgacat aataatatca
    18661 aagccttctg tgaatccatt tgttattagt atatctttat tacttatatc agcacctttg
    18721 tttgtcatat attctagtaa atagtcaata agtggcttat aaccttgtgc atatccatag
    18781 ttgagtaatt tatgtccttc tagtgaaatt ctatttaaaa aggatttttt taattcttcc
    18841 atgtcgaata aatctccatc tggagaaata cttttaaagg aaatcaggtc agagctccaa
    18901 ggaatttcac tttttataat gtccaattca ttggcttttt tagagtatgt attctctaaa
    18961 caatcccagt ttaatgacca tgtagtattt gaagacttat tctttgattt aacaaatgta
    19021 ccttttcctg aaatagagta gattaatcct tctgatttta attcttcata agttaaaact
    19081 acagaatttc ggctaacttg taaaagctga ctaagctccc ttgtagaagg gagcttacta
    19141 ttgtctggaa ttagtccttt tgatatcata tcacttatat aattttttag ttgtatataa
    19201 attggttcat tatcatttaa ttttagattt gaaaatatca aattaccacc tcaaattatt
    19261 tcatctaatt taaagagtat tctataagtt tatctaatag ttcactgtac ttaatacctc
    19321 tagcagcagc acttctagga attaaactag tttgagtcat tccaggaaga gtatttagtt
    19381 caagaacata tggaatacct tcacttataa tcatatcaac tcttacatat gctttgcaat
    19441 taaaaatttt ccaacaagtc tcagaaattt cgtttactct tttttgaagt tcttcatcta
    19501 agtaaacaac ttcttccttt gcaccttttt caactgagta tttagcttca taatcaaaga
    19561 atcctgaacc agattttata gatattgtag ggaacacttc tccatttaat atgaatgaag
    19621 tatattctcc acctggtata tatttttcaa tcattacaaa ttcatcaact tccagacctt
    19681 ttctaactgc ttcttcaact tcatccttag aatgaataaa gaatgtggct acacttgaac
    19741 ctcctgagtt tggttttata aatactggat aacctatatt atctatttca tcatagtcta
    19801 tttcatcaac tgattttact aaaacccatg gagcagtagg taggtttgaa tctctaagca
    19861 tttttttagt tatattttta tccatacaca tcccactgca aagaggtcca catccagagt
    19921 atggtatatc catagtctca agtattgatt gtatacatcc atcttcacca aattttccat
    19981 gtaaagcaag tattgcaaaa tctatatctt ctggtatctt tgtaaatata tcttttttat
    20041 catctattat tacctttaca acttcatatt tatttttatc aaggttatta tatatctctt
    20101 ttcctgaatt taaagaaact tctctctctg atgatattcc acccattata actgctattt
    20161 tcatattata cactcctatt ttaattttta ttattttaaa gtaaactttt tatttacata
    20221 cttaattata tgaattaaca gttgaggatg aaagtaccac tatatacgaa gtttatatta
    20281 tgtgtggttt tagaataata atctagttta aagatatgtt taaatgattt tttaacttta
    20341 tttatgttat tatttttgct aaaattattg ttatgaactt aagactagtc ttaatggcta
    20401 taaatcaagg agtgataaat tttgaaagct gaaataataa gtgtaggaac agaaatatta
    20461 ttgggagata tagtaaatac aaattctcaa tttttagcta aagaacttgc atcattggga
    20521 atagaagtat accatcagag tactgttgga gataacaaac aaagactttt agaatgtttt
    20581 gatgaaagct taaagagaag tgattttgta attactacag gtggtcttgg gccaactggt
    20641 gatgatatga caaaagaaac tgctgcagaa tactttggtc agaaattaga actacataaa
    20701 ccttcactag aggttttgga atctttcttt gttaaaacag ggaaaaaaat ggccgaaaat
    20761 aatatgaaac aagtgtactt tccaaaagat gctatagtac taaaaaataa taatggtaca
    20821 gctccagggg caattttaaa aaaagatggt aaatttataa tagtattgcc tggacctcca
    20881 agagaaatga aagctatgtt taatgagagt gtcaaacctt acttacaaca attcacaaat
    20941 gaaatgctag tatctaagac tttaagatta tatggaatag gtgagtcaaa tctagaatta
    21001 gaaattctcg atataattaa tgagcagact aatccaacag ttgctttata tgcaaaagaa
    21061 ttagaagtta caataagaat aactgcaaag gctgaaaatg aaagagaagc ttttaaatta
    21121 ataaaaccag tagaagaaaa aataaaaagt agagttggta aatatgtata tactgaagga
    21181 gatatttcca tatctgaagg ggagactgct ttagaagatg ctgtttctaa gctacttgta
    21241 gaaaaaaatc taacaatagc agtggctgaa tcttgtactg gaggtttggt ttcttcatct
    21301 cttataaact atccaggtat atcctctgta tttcttgaag gttgtgtaac atattctaat
    21361 gattctaaaa tgaaaagatt aggagtaaaa agagaaactt tggaagagtt tggagcagta
    21421 agtgagcaaa cagctataga aatggctgag ggcattgcta aaggacttaa agctaatata
    21481 ggtatatcta caactggagt agctggacct ggtggaggaa ccaaagagaa gcctgtggga
    21541 ctggtttata ctgcaatata tataaatgga aaaacaatag taaagaaaaa tatatttaat
    21601 ggggatagaa gaaagataag attaagagct actagagatt tgcttaatga attgagaata
    21661 caattagaaa agctttaagt tattttgatt aaagtattag taaaatataa aattgttttt
    21721 ggaggctttt aatttatgat aaaacatata ttttgtgatt tagatggaac tctatatgaa
    21781 aatggaacca taacaaaaga agatatagta gcaattgaag aaatagaaaa aaagggagta
    21841 cagtttaatg ttgctacagg tagaattttt aaacaagcac ataatattat caaagatagt
    21901 ttagatatga atggatacta tgtatgtgaa aatggttcat ttatatatga taaagattat
    21961 aatgtaattt ttaaaagaac gatagatgat aatttagtaa aaaaagtcat tgatagattt
    22021 gagtcaagtg atgcacaact ttattttaaa tataagggtg atgtaattgt gggtagtgat
    22081 accactgctt ttagacatta ttctagtgac tttatagttg acccagattt tgaaaaaaga
    22141 agtagttttg ataatcttat aggaaatata ggcgtatgtt ctgaaaattt ggaagagtta
    22201 tccagaatag aactttatct aaaaagtgag tttagtgaag tcttagaaat atatttttct
    22261 ggaacatata ctttaaatat agttcctaaa agtgtgtcaa aaagaggaag tatagagcat
    22321 gtaataaaaa cgttaaatgt aagtccagat gaagttgcta ctattgggga ttctccaaat
    22381 gatatttgca tgttagaagg atttaaatat agttttgcaa tgtctaaggc aagggaagat
    22441 gtaaagcaaa gtgcaaatta tgtagtagat agtgtaaaaa gtgccataga tgttataatg
    22501 gagataaata gttagtataa aaaatgactg tcctagagaa gtataaaatt atattctata
    22561 gagcagtcat tttaattata ttcaattata agtttatata agtttaaaaa ttcttaagtt
    22621 aaccgcaatt tgctatatga tatgatttta ttgtattaga taaaatagag tcaagtatat
    22681 aatttatgac actattacaa gtataagctg aaaattttaa atcattcata taatcagttt
    22741 tctttttgta atcactataa agtttagtaa aaaacttctc aaaactacta tttatagtac
    22801 cacatttaat tttagataaa tcaacatctt ttgcaaatgc tccaagttct ctttcataag
    22861 taacatgttt atttaagcta tgcttaaatt cccaacgttc actgtgagct acacagaaaa
    22921 aatcacataa aaaatgacat atgactccta gttcctgact aagtctactt actgaaaagt
    22981 atttagaaag actatctata ttaagactgc atagatagtt tattttagaa acaatcatat
    23041 taaatgactc atctaaataa tgttttttta atttatattt agaaaaagca tcaggcttta
    23101 tattgccata tataaaattt ttttcgctta taaagaatgt tttattttca tctatattat
    23161 caagtattga ttttgccata ataaaatggg
//
LOCUS       DAYAOY010000061        22728 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYAOY010000061 DAYAOY010000000
VERSION     DAYAOY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.061"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            27..467
                     /locus_tag="ACGI6B_002468"
     CDS             27..467
                     /locus_tag="ACGI6B_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HFG9751905.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            1112..2419
                     /locus_tag="ACGI6B_002469"
     CDS             1112..2419
                     /locus_tag="ACGI6B_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751906.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2505..2767
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2893..3696
                     /locus_tag="ACGI6B_002470"
     CDS             2893..3696
                     /locus_tag="ACGI6B_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9751907.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3734..4345
                     /locus_tag="ACGI6B_002471"
     CDS             3734..4345
                     /locus_tag="ACGI6B_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFG9751908.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            4377..5048
                     /locus_tag="ACGI6B_002472"
     CDS             4377..5048
                     /locus_tag="ACGI6B_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFG9751909.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            5347..5709
                     /locus_tag="ACGI6B_002473"
     CDS             5347..5709
                     /locus_tag="ACGI6B_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFG9751910.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            6145..6846
                     /locus_tag="ACGI6B_002474"
     CDS             6145..6846
                     /locus_tag="ACGI6B_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HFG9751911.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            7117..7935
                     /gene="larE"
                     /locus_tag="ACGI6B_002475"
     CDS             7117..7935
                     /gene="larE"
                     /locus_tag="ACGI6B_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HFG9751912.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7990..9138
                     /locus_tag="ACGI6B_002476"
     CDS             7990..9138
                     /locus_tag="ACGI6B_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFG9751913.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            9274..10662
                     /locus_tag="ACGI6B_002477"
     CDS             9274..10662
                     /locus_tag="ACGI6B_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751914.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGDDSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10691..11389
                     /gene="walR"
                     /locus_tag="ACGI6B_002478"
     CDS             10691..11389
                     /gene="walR"
                     /locus_tag="ACGI6B_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HFG9751915.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            11392..12825
                     /gene="walK"
                     /locus_tag="ACGI6B_002479"
     CDS             11392..12825
                     /gene="walK"
                     /locus_tag="ACGI6B_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFG9751916.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12847..13584
                     /gene="truA"
                     /locus_tag="ACGI6B_002480"
     CDS             12847..13584
                     /gene="truA"
                     /locus_tag="ACGI6B_002480"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFG9751917.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13695..13902
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14155..15354
                     /locus_tag="ACGI6B_002481"
     CDS             14155..15354
                     /locus_tag="ACGI6B_002481"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFG9751918.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            15601..16647
                     /locus_tag="ACGI6B_002482"
     CDS             15601..16647
                     /locus_tag="ACGI6B_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HFG9751919.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16755..17918
                     /locus_tag="ACGI6B_002483"
     CDS             16755..17918
                     /locus_tag="ACGI6B_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9751920.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(18007..18387)
                     /locus_tag="ACGI6B_002484"
     CDS             complement(18007..18387)
                     /locus_tag="ACGI6B_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HFG9751921.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(18390..19049)
                     /locus_tag="ACGI6B_002485"
     CDS             complement(18390..19049)
                     /locus_tag="ACGI6B_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFG9751922.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(19155..20129)
                     /locus_tag="ACGI6B_002486"
     CDS             complement(19155..20129)
                     /locus_tag="ACGI6B_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9751923.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(20169..20516)
                     /locus_tag="ACGI6B_002487"
     CDS             complement(20169..20516)
                     /locus_tag="ACGI6B_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751924.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(20734..21690)
                     /locus_tag="ACGI6B_002488"
     CDS             complement(20734..21690)
                     /locus_tag="ACGI6B_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFG9751925.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            22135..22503
                     /locus_tag="ACGI6B_002489"
     CDS             22135..22503
                     /locus_tag="ACGI6B_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFG9751926.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         9046 a         2286 c         3720 g         7676 t
ORIGIN      
        1 tatttgtaat gagggagatg agaagattga agaatctcaa tttacttatt aataaattat
       61 attcaaaaaa tcataatgaa gcatataaaa cttttttatt cttagaaaat gaaagtttaa
      121 aatcaaatat tacatattgt ttttttgact cattcctaga aatgattgat aacgaaaact
      181 catatattcg aacaagggga ttgttattaa tctctgcaaa tgctcagtgg gatattgata
      241 ataaaattga aataaatata gatagtatac taagtcatat cgtagataaa aaaccttctg
      301 tatcaagaat gtttattaaa tccataccta atattacaaa gtataaagaa aatcttatta
      361 atcgcataaa aatggaatta agtaatgcag atataagtat ttataataat aatatgaaac
      421 cattagtaga aaaggatata aacgatacat tatctaatat tagttaaata ttaatataga
      481 aattgaatat tatctagtta caattattta taactaagat aataaaacaa caatggtgct
      541 aatttatatg tataaattaa ttatgaaaaa tttatataag taaaatatat taacataaag
      601 gtaaaatttt gtatactaaa agggaatatt tacagtttaa tggcagtttt aaaataataa
      661 attaaagctg tcatttttat ttaaaaattt ttagaaaaag tacaatagtg ttaatgtgtg
      721 aattagataa ggtgtatata atagtttaga tgatatgata taaaatatgt tgtaaattaa
      781 atgttttctt tgacaatata tacacaagga tgataatatc tatatgaata aaagatagtg
      841 tgtaatttga taatatgtaa ttataacatt atgatataat tacaataaag tatgtaataa
      901 tctgtagaaa tttcaagcta gttatctaag ttttcggata actaaatcta aacggaatac
      961 ataatgtatt aaataaatga tgatactaaa atgatactat ttttatagaa atttattgag
     1021 gtatataatt aatgaaaata atatattaaa ttatataaat taaattatat aatatattga
     1081 gttttacata aaagcaaagg tgggaataaa aatgaaagaa attgctgatg ctataacatc
     1141 agagtattta agctatggta tcaatgaaga tattagtatc tatgaaggta gattttgcat
     1201 atatttagat aaaaaatata gatgtaatgg gaaaatttat tataaaatga cacctccaat
     1261 atctataagt tttaaagcag acataggttg tgttgaagag atagacaatg aagatgacaa
     1321 tttagcttta gattatgata atgctattct tgaagttcat ggatataaaa ttatttctat
     1381 aacaataaat actttaagtg aatttagtgt agaaggttat atcaatgatg actgtataaa
     1441 atctaagaac tcctatgttg aatatgtaga ttttaatata ataaacttag ataaaatacc
     1501 tggaaaactc attaaataca atgataaggt atacgcaggt agaattgaat ttgatataaa
     1561 tgattatgtt gtaactatag acaagcgata tgattataga aaagaattaa aatcagaatt
     1621 aaagtctaaa tctggagcaa taataactca tataggcagg attagaagaa aagatggtag
     1681 aatttttaga acaaataata caataaatct tttagatagg atatcaacag ctttgagttt
     1741 tatgtgtgga agatatgtag ggttttgttt ggccaaaggg tatagaagtg gtaatgaagt
     1801 ttatagaata tggaatgaaa atcaaatatc tccttttaga tatgtaccaa catggtcaga
     1861 tacactttca aattatcata atatggaaaa atatataagc cttatgtgta aaaagctaga
     1921 agatttttat tatggttctg ctataaagag tgttgttgat tggtacatag aatctcttgg
     1981 aagtgctacc atggaaaaca atataatatc tgtacaaatt gcacttgaaa ctttgtcata
     2041 tgtaattcta gtggaacaaa ataagatact tacagatgaa gcatttgact gtaatttggc
     2101 atctaaaaat ataagactct tacttgatac atgtaagata ccatatggta aacatgaatt
     2161 aaatatattt gataatataa taaaaaataa atttgatgat ggagtggatt tagttattta
     2221 tttaagaaat agcatagttc atccaagtag aaagactcat agagctgtgt tagaggttga
     2281 agatatttgg aatataattt caataggaac gagatatatt gaacttgttt tactctttat
     2341 tttaggttat agaggagaat attcaaacag attagtagaa agatgttatg gtgaagtgga
     2401 agttgtacct tggaactaga tttttatatt tattttaaaa tatatattga catatattgc
     2461 cttatgctat aaaatcaata tcataataat aattcagaat aacattaaat gctaagaaaa
     2521 gaaatagtaa aagtagttaa tcttaaacag agagtttccg attggtgaga ggagcaatag
     2581 attttacttt gaacacatct ttgagcagga ctctgaaaga gtagaaatac tttattagga
     2641 gatttccgga atccttgcac ccgttatagt gctagagtat aataatttta ttgtacttaa
     2701 agaggttagt gccgtgaggc attaatgaac agaggtggaa ccacgagagt taactcccgt
     2761 cctctagcag aagctagagg cgggagtttt ttaatataat tttttaagcg cgcagagaat
     2821 tatattgcaa tagatacact ttttaaaaaa taatcaaaat attttaaaaa ttaaaagacg
     2881 agggagaata ttatgaaaaa tatattaaaa aaagtaggaa tatttacaat tatgttaggt
     2941 ttattaggtg gagttgtagg ttgttcaaaa ccagataatg aaaaagataa ggatgcttca
     3001 aaggaatcta aaaaagaagt agttgtaggg tttgataata cgtttgtacc aatgggattt
     3061 ttagatgaaa agggagatac agtaggcttt gacgtagact tagctaaaga aacatttaaa
     3121 agacttggaa tggaagttaa gtttcagcct atagattggt caatgaagga aactgaatta
     3181 aatgattcaa aaactgtaga tgtattatgg aatggatatt ctataacaga cgaaagaaag
     3241 aaaatagtat catatacaga accatattta caaaataaac agataatagt tactctaagt
     3301 gactcaaaaa taaattcaaa agctgattta aaagataaag aagttggaac acaacaaggt
     3361 tcaacagcac ttgatgcagt ggaaaaggat aaagatttta tgaatagctt aaaaggtgga
     3421 gcaccagttc tttatgatac ttatgataaa gcacttagag atttagagat aggtagaact
     3481 agtgcagttg ttggtgatga agttttaatt agatactata tgggacaaaa gggtgaagac
     3541 aaatacaagg tattaaaaga cgattttgga ttagaagatt atgttgtagc aacttctaaa
     3601 gaaaatccag aactttgtga gaaaataaat gaaacattaa aagaaatgaa aaaagatgga
     3661 acttttgaca agatttatga taagtggttt aaatagaaga taagttagct gttaagaagg
     3721 gggcactaaa ggcatgttac aaggtttaga gatagttata gcaatgtttt gtataacatt
     3781 gatagtatca attccattgg ggattggagt tgcattttta agattatcaa agaataaatt
     3841 agtaagtggt attacacaat gttatatatt gataatgaga ggtacacctt tacttttgca
     3901 aatgatagtc atattttacg gattacctct tttaggaatt gtatttgaca gatttacagc
     3961 tggagtagta gctttcttct taaattatgc agcatatttt gcagaaatat ttagaggtgg
     4021 aatacagtca atcgatagag gacaatatga agcttcaaaa gtactaggat ttgataagtt
     4081 tactatgtat aagagagtaa tctttccaca agtttttaaa agaatactag cacctatttc
     4141 aaatgaagta atcacattag taaaagatac atctttagta tatatattag gattaaatga
     4201 tattttaaga atatctcaaa tagcaatgaa cagggaagca agtctgttac cattatttga
     4261 ggcaggagcg atatatctta tatttgtagc tatacttact aagggatttg aattacttga
     4321 aaaaaaatat tcttattata gataaagtta caatattata ggaggaggtt ttttatatgt
     4381 tgaaaatcaa aaacttaaat aaatcattta aaaaaaatag agtacttaaa gatatttctt
     4441 ttgagttaga agaaggtcag attggagttt tacttggtaa gtctggtgca ggaaagacca
     4501 caatattaag gtgtataaat ggtcttgaag aatttgatag tggagagatt ataatagata
     4561 atgaggtaat aaaaaataag agagatatgg caaaaatacg aggaaaaata ggtatggttt
     4621 ttcaaaattt caacttattt cctcatatga cagtccttga aaatataatt gaatccccag
     4681 ttaatgtttt taaagttcct agaaaagagg ctgaagagag ggctagagaa cttttaagat
     4741 tagttgattt agaagataaa ttaaattctt atccatttga attgtcagga ggacagcaac
     4801 agagagttgc tatagctagg tcatgtgcat tgatgccaaa agtcctttgt tttgatgaac
     4861 ctacttctgc acttgatatt gatactattc aaagagtagt caatataatg aacagactta
     4921 aagacaaagg tatgacaata ttaataatta cacatgatgt tgtcttctca aataatgttg
     4981 cagataaaat tataagtata aaagatggta tagtagaaaa tgttcaaata aaagaaaaaa
     5041 ttgtttagag ttttataaat ataaactagg gtagaatttt ataatataaa taaagggcaa
     5101 gtaaaatcaa atatactatg cccttttgtt atctcaaaat ttaatcttat attttatgtt
     5161 taagttacat tataatttat aataaaaata aaaggactat ttaaatatat aatgtgaata
     5221 aatgtttaat aatttgaaat atgataattt tttatgttga aagcttataa tgattatgat
     5281 ataaattggt tatagtatac aatttatggg tataacaata aaaaattaga aaggaatttt
     5341 tataaaatga ttaaattata tggatataca aaatgctcta ctgttaaaaa agctaaaaat
     5401 tggcttaagg aaaataattt agaatttgaa gatatagata tggttcaaaa ccctcctagt
     5461 aaagaggagt taaaatcaat ttataagact agtggatatg atataaagaa attttttaat
     5521 acaagtggta tgaaatatag agaattgggt cttaaagata ttgtaaaaac tgaatctgat
     5581 gataaacttt tagaaatatt ggtaagtgat ggtatgctta ttaaaagacc attacttttg
     5641 gatggtaaaa atgtattatt gggctttaaa gaagatgtat ggaaaagtac tttgctaaaa
     5701 gaagattagg aagagatagg tgtgttgatt aaaattatta attaatacac ctataaattt
     5761 atgtattaaa tctaattttt ataaatatta attagcaaat tcaaatcaaa aaaattcata
     5821 tcaacaaaca tattacttct tattacaaat tatatcatat gttttatatt aaaatattat
     5881 aatattacaa cgaattgtta caaaattact tattattaac catgaacatt tgttttgtgc
     5941 tatcatattt atataaggga attgtcataa aaaatatctt gttcatacag ttttatatag
     6001 aaaaaagaaa tgaatctttt ctttatataa gtatattttg taaattgcct ctatatttat
     6061 agtttaaata atagtggtat taaaaatctg acaacaaaaa taaaactttc aaaaaattaa
     6121 atctaaaaag aataggagat aaatatgagt attgaagatt tatatgattt tgaatgtgtt
     6181 cctttgaaca atgagattga tattgatgtt ttatttgaaa agatgataga agatggaaaa
     6241 gaaaaagaat atacaccaat tatagtcatt gatgaaaaag tagggctatt aagaaaaaat
     6301 atagatatga ttactaaaga gtatggttct cttgaaaact atagagagta ttgtttaaca
     6361 aaatataatg aaattgatgt aaatcaattt tttgatttta ggaagagtga gatttcagaa
     6421 ttattaaagg agctaattga tttagaagat gatttttatg attataaaca ttttagacca
     6481 attatgaata tagatatatt tcatgaatat aacaaagttt acatagctaa aattccaaca
     6541 attaaaccat atgaagtatt tgcatacata cctattggcg gatttaatga ttgtcctaga
     6601 gatgaagaac atatagctat tgcaaaatat tggtatgaaa agcatggaat ctttccaatc
     6661 gcaattggat gtgatacagt tcaatattct gtaaaaaatt taactaaaga tggtaaaaaa
     6721 tttgatgaat tatgtattga attagtattt tattgtgagg atattattat acaagggtat
     6781 gaaaccttaa aggctcttaa ggatgtatta aaacgctcta caatagtgct tttttggtgg
     6841 gattgatatt ccatcattta gatagtatta aattgttagt caccaagaat ccacttgctt
     6901 tagttatggg gagtgtgtca aactaggttt ttatacacag tgtagcatag taatatgttt
     6961 aaattacaaa ggttaaaaaa atactttaat atttttaata ttataattaa tagatttaaa
     7021 ggtaattata atttataaaa taaatacttt atatgttata atcattaaga atgtaaaaaa
     7081 ataatatata gttttatagt tcatattttt ccagatatgg aggtaaattt tgtgaaggaa
     7141 aaagaaaaat tagataaatt aaaaaaaatg ttacttgaat taggcagtgt tgtagttgct
     7201 tattctggtg gagtagatag taattttttg cttaaggtcg caaaagatac attaggtgaa
     7261 aatgttatag ctgtgactat tcatgcaatg atgcattcaa gtagggaaat tgaagaggct
     7321 aaacaatata ctcaaaattt tggtgttaaa catatcatat taaatataga aaactttgat
     7381 ttaaaagaat ttaaagaaaa tggtgttgat agatgttatc attgtaaaaa atatatattc
     7441 tctaaaataa aagaggtagc aaaagaacat aatataaagt acatagtaga tggaacaaat
     7501 atagatgatt taggagacta tagacctggg cttaaggcat tgagtgaatt aggagtaatt
     7561 agtccattaa aagatagtag tttaaaaaaa gaagaaatta ggtcgctttc aaaaacatta
     7621 ggattaaata cttttaataa accatcattt gcatgtcttg cgagtaggat accttatgga
     7681 gttgaaataa cagatgaaaa tttaagaata gtagaaaaga gtgaagaata tctatccaat
     7741 ttaggttttt ctcaatttag agtgagaatg catggagata tagctagaat agaagttgga
     7801 caggaagaac taggtaaatt ttttgaaaat aacaatttta ataaagttga tactaaatta
     7861 aaaatatttg gctttaaata tgttacacta gatatgtcag gatataaaat gggaagtatg
     7921 aatttaaatg tgtagattat tttaaaaata aataagtttt atagttaata taaggaaaca
     7981 ggaggtttta tgaaaaatta tacgttaata tcgccatgtt tttttggaat ggaaaaaatg
     8041 ctggctagag agattactaa tttaggatat gaaataataa aaacagaaga tggaagaata
     8101 acatataaaa ctgatgagtt tggaatagca aagtcaaaca tgtggttgag atgtgctgaa
     8161 agagtacatc ttaaaattgc tgaatttgag gcaaaaagct ttgatgaatt atttgaaaat
     8221 acaaaaagaa taaactggtc tagatatata ccttatggag ctcaatttcc tatatcaaaa
     8281 gcttcctcta taaaatcaaa attatacagt acaccagatg tacaagccat agtaaaaaag
     8341 gctatagtag aaagcttaaa gaaaagttat ttggaggatg gtttgcttaa agaagataag
     8401 gagaaatacc ctatttttgt atttatacac aaagataaag taaccatttc tatagataca
     8461 acaggagatg ctttgcataa gagaggatat agagaaaagg caaataaagc tccaataaga
     8521 gagactttag ctgctgggct tatttattta actccctgga aagcaggtag agttttagta
     8581 gaccctatgt gtggttctgg tactatattg attgaagctg ctatgattgg tataaatatg
     8641 gcacctggct taaatagaga gtttatatca gaaaagtgga gaactcttga taagaaaata
     8701 tggtgggatg taagaaaaga tgcttttaat aagatagata atgaatctaa atttaagatt
     8761 tatggatatg atatagatga agaatctata gatatagcta gagaaaatgc tgaaatagca
     8821 ggtgtagatg aatatataga gtttaatgta ggagatgcaa ctcaatttaa aagtgaagat
     8881 gagtttggat ttataatcac taatcctcct tatggagaaa gattagaaga taaagacagt
     8941 gttaaacagt tgtataagga gcttggttat gcatttagaa aattaaaaaa ttggtcgtat
     9001 tatttgataa cttcttatga agactttgaa tatgagtttg ggcaaaaagc agataaaaaa
     9061 agaaaattgt ataatggtat gttaaaaact aactttttcc aatatccggg tcctaagcct
     9121 cctagaaata ataaataagt tttacatgat ttatttgtaa aatataaaat tttattaaaa
     9181 taagtaaaac attatgatgc cttgaaacgt ctaattattg tgaggagatt tttgtgaaca
     9241 aatttaaaga tttataggat taggaggagt taaatgaaag tgttaaagct tattactgta
     9301 gtagccctga tgatgttatt tgttactgga tgtaatataa aaactgaatc tccagaacaa
     9361 ttagcgaaga tacctgacta tgatgataca aaaaaagtat tgtatgatgg tattgaccag
     9421 ttactaaaac cagattcatc tatgatattg ccttctaata caaaagaagt aggaaaaata
     9481 aataaggtag atttaaacag cgatggaatt gatgaattag tagtatttga acaaaaagaa
     9541 gatttaagta ataatgtgag tcaggtaggg tttgtaacct tgagttacaa tggagaaaaa
     9601 tacgtacttg gagaccattt tttagaaaat ggcgaatcaa tagaatatgc taatttttat
     9661 gatttggact cagatggata taaggaagtt atcttattgg ttaaatccaa agacaaaacg
     9721 aatatgcata tatataaagt agtagataat gaaataacaa aaatatatga cttggatgca
     9781 tcatggcttc aaaataaaga agattttaat gatatgaaag taaaaattgg atatatagat
     9841 ggagatgaca agttagacat attgatgctt catttgaata ataagactaa tgaaatgttt
     9901 gcaagtgttg caaattttga tgggaaaatg aaaatcaaag attctgttaa gtttgaaaat
     9961 gtaaaaaact taagtgaatt atatataaca ttaggtaatg ttgcttcatc tgttggaaat
    10021 tcttctgcag ctataaaagg tattgtactt gatattccta tattgaaaga taataactat
    10081 attacgcaga tactttatat gaaagatggg aaaattaaca aagcttttag tgattatgat
    10141 aaaactatta ctaaatctta ttatatacct gtagatgata ttgacaaaga taagattata
    10201 gaaataccta tagttgcagg cagtacaggt aataataaaa atacttatag ttctaaatca
    10261 tctgcaacta taagttggta tagatggaat ggaaaagaag gtgatgactc aagtttaata
    10321 tttacaagtc agatatatta taattataaa tataatttta aattatttat tccaaataat
    10381 ttatttgata aaatacttgt tgaacaggaa tttgttggag aaaaagcagt atttaaattt
    10441 tactattttg actattcaga ccgtaagaat ttatttaata tagtagtaga gagtaaaaat
    10501 aaacttgaag atagaaaaaa tacaggtact caaaattcta ttgtacttca agagagcgat
    10561 gaatatactt tcttactagt tgtaaacaat gcaaatgaaa tggataagct agatatgact
    10621 atagacgcat taaaagaata tttctcatta atatatggtt aatattatac tcaattggag
    10681 gttttatttt atgaaggaaa agatacttat actagaagat gaaattggta ttagaagttt
    10741 tgttagtata aacttaaaaa gggaaggcta tgaaatagtt gaggcaggta ctggaagaga
    10801 agctatagaa aaaatgacaa cagaaaagga tattacaata gctcttttag atgtaatgct
    10861 tccagatata agtggtatag aagtatgtaa atttataagg gagaattttg accaagtagg
    10921 tataataatg cttactgcta aagctcaaga agatgataaa atagaaggat ttatctcagg
    10981 agcagatgat tacatcataa aaccatttag tataaaagag cttttagtca gagtttctgc
    11041 tcttcttaga agagtagcaa aggatgattc aagtgttaag tcgagtgaga tagtttctcc
    11101 accatttata cttgatatag acaaaagaaa attatttaaa aatggaaagg aaattgagct
    11161 tacaccaact gaattttcaa tagtgaaata tctaatatca aatgctaagc agtcgttaag
    11221 tagagaccag atattagacg aagtatgggg aactaactat ttatatgatt ttaaaatagt
    11281 agatgtaaat ataagaagga taagaaataa aatagaagat gacccatcaa aaccaaaata
    11341 tatacaaact atttggggtt atggatactg ttttagaaag gaagaataat agtgttacat
    11401 tttgaaggtc tgagaaaaaa agtaataaaa aactatttta taataattat tataatggtt
    11461 actctgtttg aaggattgtt catgttttac atacaaaact actattatga ttctgtcaaa
    11521 cagctacttg aatctgaaat aaaatatgct gatgaatata atgctattac aatggaaact
    11581 acaagttttg agaaaaaagt aaaaaatata tttgataaac aacccttaac taaaaattca
    11641 gaatttggaa tttcaataat agataaagat aaaaatatta tattagacca atatggtttt
    11701 aaaagtaaag aaaaagcaaa ctatgaagat gttaacaatg cattaaaaga tataaaaact
    11761 aaaaatttaa caccttatac ttatagaata ccagatacag gggagcatgt tatgagtatc
    11821 tccttaccac ttaaggtaaa taatataata gaaggtgttg ttagatatac agtatcatta
    11881 gatgctattg ataatgcaat attaaagcaa gctacatggc ttatattagc aggaatattt
    11941 atacttatta tagctatact aataagtctt aaatttgctg agactcttat aaaaccatta
    12001 cgagagctaa agaaatttgc aaatgaattg gctgtaggaa attataatat aaaattagaa
    12061 aagatgaaga ttgtagatga tgaaattggt gatttagcac agacatttga acacatggca
    12121 catgagattg acaaaagtga aaaattaaaa gaggaattta tatcatctgt atctcatgaa
    12181 ctaaggactc ctcttacatc tataaagggg tggagtgaaa ccttaggtta tgaaagtata
    12241 acaagagaag aactagactt aggtttaggc attatacaag atgaaacaga aagacttata
    12301 aaactagttg aagaattatt agacttttca aggctttctt cagacaggat aaaacttcat
    12361 gttgatatag ttgatgttga aggtttaatt gtaggtgttg taaatcaact taaagttaaa
    12421 gcagctgaaa aagatatatc acttttattt gaatttgaaa atgaatttat agagaacatt
    12481 caaggagata aaaatagatt aagacaggtt ctcataaatt taatacaaaa ttcatttaaa
    12541 tttacgagtc aaggtggtta cataaaagtt gtagcttctc aagatgaaga gataacaacc
    12601 atatcagtag aggataatgg ttcaggtata gaaaagcaaa atttaaataa agtgttagat
    12661 aaatttttcc aagaagatta taataaggct ggtagtggat taggactagc aattagtaat
    12721 gaaattgtaa aacttcatgg tggaagaatg aaaatagaaa gtgaaaagaa tgttggaaca
    12781 aaaataactt ttaatataaa aaataaattt gctaaacaag cataaattct tagaaagagg
    12841 attaagatga gaaacataaa aatgatagta gcatacgatg gttcaagata taaaggatat
    12901 caaaaattag gtgacaataa tatgactatt caagaaaaac ttgaaaatgt tttaagtaaa
    12961 atgactaatg aaacagtgga gataataggt tctggaagaa ctgatatggg agctcatgca
    13021 agaggacaag tggtaaattt tagaactaat tgtatggatt ctttggataa aattcaaaaa
    13081 tatttatatg aatatttacc agaagatata gttgtaaaaa ctgttgaaga agttgatgaa
    13141 agatttcatt caagatacaa tgtaaaatca aaaacttata tgtataaaat tgacaataat
    13201 aagtatcata gtccttttat aagaaaatat gctactcatg taagtaaaaa attagattta
    13261 gatagaatga gaaaagcaag tgagtattta gtaggagaac atgattttac cagttttgct
    13321 tcttctaaat ctaagaaaaa atcaaatgta agagaaattt attctataaa tataaaagaa
    13381 gatgataatg ttatagaaat atatgtagaa ggaaatggat ttttgtataa catggtaagg
    13441 ataatagtag gtgctttaat agatgttggt ctaaaaagaa gagctccaca agatattaag
    13501 tatatgcttg aatcaaaaga tagatgtcaa tcatcagata ctgcaccagc taaaggacta
    13561 tgtctttgga aagtaacata ttaatcaatt atataaaact ctctttaaaa taaaaataaa
    13621 ataaattata tagttgacat aaatggaaaa ataaactatt atatatttta taagagagat
    13681 ataaaacttt atatttaaat cctgtgagaa agagagtaac ttctatgtgt tttatagaga
    13741 gctgaggatg gtggaagctt ggcaaataag tattagtgaa aagaactttg gaggatacta
    13801 attgaattta gtatgttagt aggtaatctc gccttaagag aaagagtgga taagtttatc
    13861 ttatcaatta gagtggtaac gcggatatat ttcgtctctt ggtttttcca agaggcgttt
    13921 tttaatatat ttaattatta gtgtttatct ggtatgttga acatttacta attgattagc
    13981 taagtttatc aataagagtg gtaccgcgga tatttcgcct cttagtattt taaaatacta
    14041 aggggttttt ttagtattaa aatgatttta tgtgagtcta aattctcaga gtcattcaat
    14101 attaagaatt tctataaaat aaaaattata atttaagatt gggaggaatt aaaaatgaag
    14161 gaaaaagtag tattagcata ttcaggagga cttgacacat caatcataat accttggtta
    14221 aaggaaaatt atgaagatat agatgttata gctgtttgtg gaaatgtagg tcaggaagat
    14281 aaaatggaag atgtatatga aaaagcttta caaagtggag catcaaaggc atatgtggat
    14341 gatataagtg aagaatttgt aactgaaact atatttaaag ccgtaaaagc agaggctaaa
    14401 tatgaaggaa aatacttatt aggaacttct ttagcaagac ctataatagc taaaaaatta
    14461 gttgaagttg ctcataaaga aggtgcaaaa tatatatgcc atggatgtac aggaaaaggt
    14521 aatgaccaag ttagatttga agctacaatt gcagctcttg acccgactat aaaagttatt
    14581 gcaccttgga gaatttggga tataaaatca agagaagatg ctatagacta tgctgaaaaa
    14641 cataatataa aggtaacagc aactaaagct aaaatatatt cagtagatgc taacctttgg
    14701 catgtttcaa cagaaggtgg agatatagag cacctagaaa atgaacataa aaaagatgtt
    14761 tataaacaat gtgtagaccc agaagatgca tgtgatgtag ctgaatatgt agaagtttac
    14821 tttgaaaaag gtgtacctaa aaaggtaaat ggtgaagaat tatctccagt tgcactgatt
    14881 cataagttaa atgagttggg ttgtaaacat ggaattggtg ttatagatat agttgagaat
    14941 agacttgttg gtatgaaatc aagaggtata tatgagactc caggaggaac aatcctttat
    15001 gaagcacata atattttaga aagtgctact ttagacaaag atacattaca tttcaaacaa
    15061 atggtatctt ataaatacgg agaacttatt tacaatggac tatggtattg taaattaaga
    15121 gaatctatag atgcatttat ggaacaaact caagataatg ttacaggaac agtaaaagtt
    15181 aaactttaca aaggaaatat aaaacctgct ggaatattta ctgaaaatgc tctttatgat
    15241 gaaggaatat cttcttttgg aaatagtgaa ctttatgacc acaaagatgc agaaggattt
    15301 attaatttat tcactttacc attaaaaatc agagctatga aagctggaaa ataataaaat
    15361 aaattattta agagtgtata aaaactattt ttaggttttt atatgctctt tttttatttg
    15421 aatttattaa cttatacagt aaatagtata aaaaaatata ttatagttcc aaatggctta
    15481 ataatgtgaa aatataagac ctaaaatata aattttataa atacaatagt tattgactct
    15541 ctcattgtaa gagggtttat actgaatcta atgaaaattt atagtgagga gatagattat
    15601 atgagaaaaa taattgaaat taaacatcat tcacatgatt atgagtgtat gtggaatggt
    15661 atagaagatt tatatataaa taaaaccggt gaatgccttc caaacgattt tttcttttta
    15721 ctgtcaggtt ttggttcatt ttgttatatg aaaacaaata aagctgattt gaaaagaatg
    15781 gtagctttgg gagatggaag gacaaaacag atgtataaat ttttagcacc aattgttgga
    15841 tttgaataca aacatcatga gtttaaaaag tatgagcaag cattaaagaa agcaaaatct
    15901 gaaattgatg atggatatcc agttatttta ggagctttag atatgtatta tctaccatat
    15961 tataagaagc tatatcataa agaacatatt ccttttcatt atatattaat ggtaggatat
    16021 gatgatgaag gaatatatct gtatgattgt ggtagaacag aattattaca tctttcttat
    16081 gataaattac gtgaaagcat gaattgcaat tcccctggat taagcaaaga aaatactatt
    16141 tgcacaataa gaatgaatac ttcaaagaat aaatataaca ttgctagaga agctattgct
    16201 ataaagcgtg atatgttttt aaatccacct aaaggatttt tgggatataa aggttttgaa
    16261 aagtttatta aagagttgcc acagtggaac actgaattgg ataaagatga ttatgataag
    16321 attttgacca atatggttat gttttttgga acagtaccaa caattccaaa tgcaatcaaa
    16381 ggaattaaag aaccagatac tgttcaattt aaaggtggat ttgataaaat gagtagaatg
    16441 ctcaacagta ttggggagga atacaaaaac gactctttta tacaagtagc tgatattttt
    16501 gataaaactg ctattataat tgagaaaata tccaatatta taatagatta cttaactcaa
    16561 acatgtgatg atacaggaca acttccgtta ttgttctctg aggtcttaga gcatatgaaa
    16621 acaggatatt taatcttgga tgtataaaaa atatattgtt gaccctaaca ttacgtaata
    16681 gtgtaatatg aaaatatagc ttgaatatat tagtaggtat tgatggctgt aaaattaaaa
    16741 tttgaggtgt ggatatgaga acagtgaaac aagtttctga tttaactgga ataagtgtga
    16801 gggcattaca ttattatgat gaaataggtc ttttaaaacc aaataaaatt acagatgcag
    16861 gatatagact ttatgatgat gaatctatta aaactcttca acaaatttta ttttttaagg
    16921 agattgatat tcctttaaga gaggttaagg aaataatgtc aagccaatat tttgataagg
    16981 ttgaagctct aaaaaatcaa aaaaaattac ttattttaaa acgaaagaga ctggatgaat
    17041 tgatagagct tataaatcaa actctaagag gagaaggaaa tattgatttt aaagagtttg
    17101 atatgagcga gtattttgat gttttagaag aatttaaaag agagcatagg aataaggtaa
    17161 ttaaaattta tggtagtgta gaaaaataca atgaatatat agaaagagtt aagtctaatg
    17221 aagagaaaat tgctaaaatg gctataaaac aatatggaac tattgagaag tttgctaaag
    17281 ctataaaaac aaattttagc agtgatatat tgaatttagg agaaaaattt gatagatata
    17341 aaaatgattg tttaaaagaa aaacatccaa aattaaaaga attatataga aagcttgtag
    17401 aagatttaaa taggaatcct tcttcaacag accttcaaga aatagcaaaa gagataacag
    17461 atatatctaa aaaagattat gaaattttct ctatggatac tggtgatgac aactggtatt
    17521 atatggttca aaattatcta gtaaatccta tgtggataga agaagtagat aaaaagtatg
    17581 gaagtggagc aggtaagttt attggacaag tactaaagac ttatctaaga gatagaaaac
    17641 caaagataaa tacattgtat gaaaaacttg tagaagattt gagtagagat tgttcttcaa
    17701 gggaagttca aagcattgtt gaagaaatag acaatgaaat gaaaagaagt aatgaatttt
    17761 ataaaataga caatggtgaa agatactttg attatatgtc agagctttat ttacaagatt
    17821 ctaactatat aaaagtaact gataagaatt atggtgatgg tgcatctaag tttattggag
    17881 aagcttttaa gatttatttt gataataata attgttgaaa atttggaagt aaaaaatatg
    17941 caagttttat aaaagcacaa aaataaggat gtagattagc tccatctata tccttattta
    18001 aagttattaa aatatatagt ttacatcaaa cttaacactt tcaataatat ttcccaaata
    18061 tgtttttata ctttcaactt cttctaaaga ttttaattta ttttcagtat aaattgcttt
    18121 gtccagaata ttaaacctta atcctatttc atcaaagtac tcatgattta aaactttgta
    18181 ccaatggttt ttttctttat tccaactcgt agatattgaa tcagcttctt tttgagcttt
    18241 ttcccaatct tcattttcta tagatttttc taaaacagat atattgtctt tatatccttc
    18301 tgtaaaatca tatagtttat tgttaacata aaatccaaat aatacaaata agatagtcca
    18361 tagtataaca aatgttgtag atttcaatat taatcaccct ttttattttg aataaatatt
    18421 ttgtcatttt catctaaaac acaaataaga acatctttaa aagtttctat gtgattagat
    18481 tttaaaatac ccattaacca gttagtatct ttttgagcta tgtctagatt tttatttatt
    18541 attctaccat ccagtataag tgggataggt aaatgattaa aagctcttgg aggagtacta
    18601 ttgtagcttg atgctggtac aacacttaga ttaccatcag tctccaaaat agcatattgt
    18661 acatctttta ggttaaagta tccttgaatc ctaagttgtt ctaataattc atctatacta
    18721 attctttctt ttttaagctc tttatataca atttttcctt tatctattaa tacagaaggt
    18781 ttacctgata aaaatgaact taattttcta ctttttaatg ataagaatga aattagagtc
    18841 tgcatgatta caagtccaga tattgctgca acaccattta tgattggtat attattattt
    18901 tccattggga cagatgcaac ttctgcaatt aatagtgtta taactaaatc aaaacagttc
    18961 atttcagcta tttcaccttt tcccatgact ctaagtgcaa ttagcactgt tatgtataat
    19021 ataatacttc ttattaatac tactgtcata tctatcaatt actcctttaa aaaatattta
    19081 cacttagtta gtatatccta tataaaaaat ttaattcaaa gtataaaaaa ccttaaataa
    19141 ttggattcaa ctatttaagg tttttttgat aattttaata ttaaattttt tatggatttt
    19201 ctgttacttt tatttatttt aataacagat tcaacaaaac tctttttatc tcctaatttt
    19261 aagataaaat caggatattc ttcaaattca ttgagtttta ttaatttgaa agaagaagat
    19321 agatttttaa agttaataat tttagagtta ttcactaata cacttctttt tttaaaataa
    19381 atttcttttt tacttatatt tagtatagtt aatatttgtt taagatatat tattatatct
    19441 tctggagttc tatcaaattt gagttcaaaa ttttcatctt caaagtaggg aatataatat
    19501 aaaggtgttt tgagagcagg taatttatta tataattctt caagaatatc gatattttta
    19561 ggggagttaa agaaaaagca tttacataca ttcattaaat ccacagcatc tgaaagtgag
    19621 ttatgtttaa tatctccttc tacagaaaaa atttttttca tattcagtaa agatatttgc
    19681 ttagataaga ttctattatt gtatgtaata tgttgagata taaaaggttg aatgtcaatc
    19741 atcatattaa ataactctaa gcatttacct gtatatttat atactctaaa tgaagttaga
    19801 aggccagtta tatcaaaatc accccaatta tatattgtaa catcatcaaa tttacaaaac
    19861 catttataaa aatcatccat aacatataaa agattaggag cattattgat atctagttgg
    19921 ttcaatttgg ttaaattttt acatttattt gaaagtattt tattgtattt tggcttaact
    19981 aaagagtaat aattatctat tgtattaaat tcgttatcaa ctaaaactcc accaatagaa
    20041 attaattccg caccattata ttcttttggt ttatttttat tatcatctag tatattaaat
    20101 tcaaagtcta taaataactt gtatttcata aatctaaagc acatcctcag gtaaattatt
    20161 tattgagcct aagcatcata agaatcaata gatataatga ctctaaacat caaaggttca
    20221 ttacaacttt tatttatttc ataatttaat tcaaataaat cttttaagat ataatttata
    20281 agtattcttt cccaacccat ttttaaatta actttagagt ctattaatat attttctatc
    20341 tttttaaata tttctatatt tgctggtatt atactgagct caatattact tttaaacggg
    20401 ttagactgta aatctgttat aagtatattt tttaattcct ctgtaagttc agaaattaaa
    20461 ctatcaccta tgtatttttt tatgatttcc tctctaattt cttttaaatt tagcaatatt
    20521 agtacctgct acttacatag tagcttttcc tttcctttta atactattat tcatttttga
    20581 atcttattta gatatatttt aagattaaaa gtctttagct atataatttt ttacccattt
    20641 ttaacttata ttaacatatt acaggcttat atctccaccc tgtttacatt gaggatggag
    20701 tatattgcct attttgataa aaatgatagg ttgctatttg cctattcctt tttctgataa
    20761 tttttctaac atatctttgg tcattttatc taaatcataa ctaaacttaa agttccattc
    20821 ttttactgca ctactactgt ctagagagtt aggccaagaa tcagctatac tttgtcttac
    20881 agggtctaca tcatattcta tcacaaaatc aggaatatat ttttttatag aatcagctat
    20941 ttcctcagga gaaaagctca tagcagtaat attaaaagca tttctatgga tgagtttatc
    21001 agctggagct tcgattaaat ccactattga ttgaagtgca tcaggcatat acatcatatc
    21061 catttttgta ccttctgcta tgtaagattt atatctctta ttttttaatg cctcataata
    21121 tatatcaact gcataatctg tagttcctcc accaggaggc gtaacataag aaataagacc
    21181 tggaaatcta acacctctag tgtctactcc aaattttgag tgataataat cacatagtaa
    21241 ttctccagaa actttagtca caccatacat agtattaggt ctttgtaatg tatcttgagg
    21301 agtcatattt tttggagaat tttcaccaaa agcagcaatt gaactaggtg taaaaaattt
    21361 acaatccaat tctttggcta tttcaagacc atttattaat ccattcatat ttaagttcca
    21421 agcttctaga ggtttacttt cagcaactgc agaaagtaaa gatgctagat gaattatagt
    21481 atctacatca tatttttttg caatttcaaa aaaacgattc ttatctgtaa catctaatat
    21541 ctcaaatata ccagattcac atactggatt accttctttt actctccttg aactagcgat
    21601 tacattttgt tctccatact catttctaag ctttattgtt aattctgaac ctatttgtcc
    21661 taatgcacct gttataagta tttttttcaa ttttacaacc tcctaagggt ttaatttatg
    21721 tagtactact tatttttatt ttagcaaatc ttttgtttaa atacaaagaa ttaaagataa
    21781 taatatacga cttaattgaa tgaagtgcag taagtgccta aaaatttaaa gctaatatat
    21841 aatggtatat tgtataacaa taaaagagta gattaaatta ttatatatag attgtgtgac
    21901 taagtcacag aaaatagagt taatttaata tataataaaa gcatatcaag taaagcttta
    21961 aattaaagta actttataaa taaaaagtaa tataaattga taaaattata aatttattta
    22021 tagtgaatat gggttggatt ttatagatac aaaaatagta tatatgatat gtagaccatt
    22081 agcaggttta aagtagcaaa acatataaaa taaaatttga aaggaagtat aaaaatgaca
    22141 agaccatcaa tatatcatag ccaaggatac acttgtgcag aggcattaat aaagtcttat
    22201 aatgaggaac ataacacaga tataccaatc tcaataggaa gtggtatggg ggttggaatg
    22261 aatgttggaa gtgtttgtgg agctgtaaat gctgctgttg ttataatagg gtatttaaat
    22321 gggcgaaata gtaatttaga tgaaaatata gcaaggaagt attcaagaga gctattgaat
    22381 agagtaagaa ataaatacag ctcagaaata tgtgtatctc ttaaaaaaaa taaagtaact
    22441 tgtgaagaaa taataaattt cacatatgat gctctaaatg atatgctaaa aaatcaaaaa
    22501 taattctaag aaacaagtat tttacattcg atagaatact tgttttttta tgatattttt
    22561 aataagtatt agtttattat aatttttaaa tcaattttat tatattttaa ttgaaacaat
    22621 ctactttgaa aattactaac tgacaaaaga atatgattat agtatagaaa aaatgtaaat
    22681 tatttaatat taagtgagag tatatgttta cctatataaa atcgtata
//
LOCUS       DAYAOY010000062        22638 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYAOY010000062 DAYAOY010000000
VERSION     DAYAOY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.062"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            223..1659
                     /locus_tag="ACGI6B_002490"
     CDS             223..1659
                     /locus_tag="ACGI6B_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFG9751927.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(1751..3625)
                     /gene="asnB"
                     /locus_tag="ACGI6B_002491"
     CDS             complement(1751..3625)
                     /gene="asnB"
                     /locus_tag="ACGI6B_002491"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9751928.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3798..4502
                     /locus_tag="ACGI6B_002492"
     CDS             3798..4502
                     /locus_tag="ACGI6B_002492"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFG9751929.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            4615..5151
                     /locus_tag="ACGI6B_002493"
     CDS             4615..5151
                     /locus_tag="ACGI6B_002493"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9751930.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5317..5937)
                     /locus_tag="ACGI6B_002494"
     CDS             complement(5317..5937)
                     /locus_tag="ACGI6B_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFG9751931.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6251..8389)
                     /gene="vanT"
                     /locus_tag="ACGI6B_002495"
     CDS             complement(6251..8389)
                     /gene="vanT"
                     /locus_tag="ACGI6B_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFG9751932.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8407..9213)
                     /locus_tag="ACGI6B_002496"
     CDS             complement(8407..9213)
                     /locus_tag="ACGI6B_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9751933.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9210..10310)
                     /gene="vanG"
                     /locus_tag="ACGI6B_002497"
     CDS             complement(9210..10310)
                     /gene="vanG"
                     /locus_tag="ACGI6B_002497"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFG9751934.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10571..11653)
                     /gene="vanS"
                     /locus_tag="ACGI6B_002498"
     CDS             complement(10571..11653)
                     /gene="vanS"
                     /locus_tag="ACGI6B_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFG9751935.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(11703..12404)
                     /locus_tag="ACGI6B_002499"
     CDS             complement(11703..12404)
                     /locus_tag="ACGI6B_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFG9751936.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12838..15369)
                     /locus_tag="ACGI6B_002500"
     CDS             complement(12838..15369)
                     /locus_tag="ACGI6B_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9751937.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15477..16094)
                     /locus_tag="ACGI6B_002501"
     CDS             complement(15477..16094)
                     /locus_tag="ACGI6B_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFG9751938.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16456..17340)
                     /locus_tag="ACGI6B_002502"
     CDS             complement(16456..17340)
                     /locus_tag="ACGI6B_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFG9751939.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17531..18781)
                     /locus_tag="ACGI6B_002503"
     CDS             complement(17531..18781)
                     /locus_tag="ACGI6B_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9751940.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(19003..19767)
                     /locus_tag="ACGI6B_002504"
     CDS             complement(19003..19767)
                     /locus_tag="ACGI6B_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9751941.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19761..20486)
                     /locus_tag="ACGI6B_002505"
     CDS             complement(19761..20486)
                     /locus_tag="ACGI6B_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751942.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20488..20856)
                     /locus_tag="ACGI6B_002506"
     CDS             complement(20488..20856)
                     /locus_tag="ACGI6B_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9751943.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(21032..22576)
                     /locus_tag="ACGI6B_002507"
     CDS             complement(21032..22576)
                     /locus_tag="ACGI6B_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9751944.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7805 a         3821 c         2365 g         8647 t
ORIGIN      
        1 tagcggtatt agattacaag cattattcta acttttttta tattaagacc ttacctttca
       61 tccctacacc taaaggagag ggcttttcgg caattttatt gtaattatat atcatttata
      121 taaacaagta catattttat tttgaaacta caaaattatt gtactatatt gttttaagtt
      181 gttttataat agattatatc atcttattat aggagaaaaa atatgatttg tactattaaa
      241 ccattaaaga tttcaataaa aaagtctact gaacctctaa ttgaatttat atctgatact
      301 acctataaaa ataagagttc ttcatatatt aaagaagtct actttacaag atatataggt
      361 gttgatgatt caattgataa atacatagaa aaaattaaaa atatagatac ttattttttt
      421 aataatccta caattcccta tattagatta aacaatctaa aaataaattt tgacaaagaa
      481 caaacagata agatgcttaa aatttttaat atttataata gttctaagtt taacagttgg
      541 gatttatata atatcgactt tccatgtaaa atagaaaatg acaccttaaa ttggactaaa
      601 aagattgcat ttaaaaatac tttagattta ttttccacaa gtatgccaaa ttgtacatcc
      661 tctatcatca aaaactttgc agttaaactt ttgtgttgga tagactattt tctacctaaa
      721 ttattttata acaaggtgaa gactactatt tcaccaaaga tagtatactt tggaaatatt
      781 aaaaggcaag aacttttttt cttatatttt ttatcacaac ttgggtgtga tattttatat
      841 ataaatccaa ataaagatat tcttgattta tatcctgaat gttcaaaatt ttctactcta
      901 tttgaacttt ctaggaagac tccaaatata ttagaaatac cttttgaaag gcatattaca
      961 aatataaatt acagtgaaaa taccaataag acttctccta ctacgaataa acctaactct
     1021 aaaaatacac ttaataacac aaatctaaca tctaaaaaaa ttgtacaaga tgaggaagta
     1081 gaactttctt atgaagacct tgcaaagtta tcagcttctg ttgttatgat tgttgtgtgc
     1141 aataacgaaa ataaaccgtt taagagcggt tctggagtag taataaataa tgaaggttat
     1201 atacttacga atcttcatgt agtcaatgat gggtattcat ttttagttag atttgaaaat
     1261 gatgataaag tttatacatc ttaccaaatt ataaaatacc attcagatta tgatttagct
     1321 gtgataaagg ttgatagaaa atgcaagcca atacctgtta aagtcagtaa aaaacctgtg
     1381 agaggtcaaa aaatagttgc aattggaagc cctctaggtc tttttaatac agtttcagat
     1441 ggaattattt cagcttttcg tgattttgaa acggttcaaa tgatacaatt tacagctcca
     1501 atctctagtg gtagttcagg aggagctctt ttagatatgt ttggaaactt actaggattg
     1561 atttccgctg gatatgatga tggacaaaac ttaaatttag ctgttgaaag tagtcttgtc
     1621 aaaatatttg ccaataattt tattgagata gtaaactaat caatcaaaat tctacttatc
     1681 ctattccata ataatcaaga gggtgaaaac attgcatgct tttgccctct gattaatatt
     1741 gaaaaaagta ttaatactcg acatttttat gctcaataaa taattgatgc catagacaaa
     1801 atgtaaatat agtccatatt tttctcgaat tatctctatg acctttaaga tgcgtatcta
     1861 aaagttttat tgcatatttt ttatctataa attcatctac atttgaatta taaatagttt
     1921 cctttacaat atcccctaga gaatctttta accaaactct tattggagtt ggaaatccta
     1981 attttttcct ttgaacaaca tgttctggga ctatttctct aaacgcctct ctaagtaaaa
     2041 cttttgtatt attcttattt attttttgct ccattcttaa attttttgcg acatcaaata
     2101 catctttata taaaaatggt actctaagtt caacctgctc agctgtagaa agtttgctaa
     2161 ttttttgaag tatatcgcct tgaagccatg tattcacatc tatatgttgc attttactta
     2221 tataatcgta attatttttt tgtgcatctc tatacaaatt agacaacagg tattcatata
     2281 tattcttttc cttatactta aaaaagaatc tttttacttc gctgttttca aaaacttttg
     2341 catttcctat atatcttttt tctaatggtg ttgtagctct ataaagataa tttcttcctt
     2401 ttatgtttgg cataagttct gaaactcttc ctaatatact tttaatataa gtaggtgaat
     2461 tgacaactga tttcatagta ctatactctt tatatatatt atatcctcca aataattcat
     2521 ctgctccttc tccagacaat gctactttaa catgtttacc tgcctcttta attaagaaat
     2581 aaagacctac ttctgaaggg tctgctatag ggtcatccaa agaataaatt acattcggta
     2641 atgattttat atattcatct tgtgttatat taatctggat attttctata cctaacttat
     2701 cagctgtttt ctttgctact tctaattcac tatatccttt gacactaaag cctgcagaaa
     2761 aagattttat atttggatta atttgtgaag ccacagtagt tataatagat gaatctattc
     2821 caccagacaa aaatgttcca atttctttct cctcttccat atgagttact attgaatcta
     2881 ctactatatt tcttatatca tcctcattca catctctatt gcctaagtga tttttagtat
     2941 aaaaaaattt tgtactactt ctaaactcaa gtttattata tctctttaaa gatagtatac
     3001 cattttctac cctaaaatag tgacctgctg gtatcagcct tattccttgt atcatagtat
     3061 cttcaggaag tacatattga aatgaaaaat aactttgtaa gcttctttca tttatattta
     3121 aatttttcat atattcaagt aaaaacttat actctgagga aaaaattatt gcattttcct
     3181 tatttatata ataaagtggt tttatcccaa ataaatctct tgctccaaat atactttcat
     3241 tttctctatc atagattata aagttgaaca ttcctttaat cttatgtaca caattcttac
     3301 caaattctat ataagatgct aataagactt caatatcatc atttgtagta aaattgtgtc
     3361 cttttttaat taattcttct tttatttcat gatagttata tatatttcca ttaaaaacaa
     3421 taacttttcc atctttttca aatggtttat ttccataaat agcatcaatt acattaaatc
     3481 tttcaaaatc aaaagttttt tcctcatcct tatactctat tctactagaa agtcttgtat
     3541 tatttacaga ttcttttatt ttaaatttaa tatcttcttt tttagatata tcacctttat
     3601 aataaattga aatattgccg tccatataat caaaattcct ttctgtcctt tagtcttatt
     3661 ctatatttca tagttttgtc tttggcttta aaacttattc aatttaaaat gaaattgcta
     3721 ttaaattctt ataaattatc tgttaatatt tcatattttt tttatttcta gaaatactat
     3781 attaggaggt gatttttatg aagaaaaaaa tattaatacc agttattatg tctttattta
     3841 taatctcaca gtgcataact tcatttgctt ttacacctga aaataacaaa tttaaggtta
     3901 aaccattacc ttatgcatat gatgcacttg aaccttatat agataaagaa acaatgaaac
     3961 tgcatcatga taagcattat caagcttatg ttgataaatt aaatgctgct cttgaaaaat
     4021 atcctgagct ttataattat tctttatgtg aattattgca aaatttagat tctttaccta
     4081 aagatattgc tacaactgta agaaataatg caggtggagc ttataatcat aaattctttt
     4141 ttgatataat gacgccagaa aaaaccatac cttctgaatc tttaaaagaa gctattgata
     4201 gagactttgg ttcttttgaa aaatttaagc aagagttcca aaaatctgct ttagatgtct
     4261 ttggttctgg ttgggcttgg cttgtagcta ctaaagatgg caaattatct attatgacta
     4321 ctccaaatca ggatagccct gtaagtaaaa acctaactcc tataatagga cttgatgttt
     4381 gggagcatgc ttactattta aaatatcaaa atagaagaaa tgaatacatt gacaactggt
     4441 ttaatgtagt aaattggaat ggtgctttag aaaattacaa aaatttaaaa tctcaagatt
     4501 aattaaatat taaaatcctt ttgaaaaaaa cttatgtttg ctatataata taataaaaga
     4561 tagtctaaat ttaccaatat aatgtttata gtgacgattc aggaggattt ttatatgata
     4621 aaagaaacac atgaaagggg aggttatata ttcgcacttt tgatactccc ctttataaac
     4681 aatctatacc tagttaaata cgatattgca tataaaatag tattaattat aatttatatt
     4741 tattttgcat atttaggttc tttatttcca gatatagata tgagaggttc ttacataagt
     4801 aaaaaattta tgttaatata caaattattt ggaagtaggt ttagacatag agggtttact
     4861 cacagtctaa ttgccttatt acttatttat agttttttta aatctctgac aatcttcact
     4921 aacaataata tagttttctc atgtttgtcc agtgggttta taataggata tctatctcac
     4981 ttgtgtcttg accttattac taaagaaggt attgaattgt tttatcccat taccataaac
     5041 atttctttac tacctataaa aactaactca aaaacagaaa aattcatatc taagctatta
     5101 aatttcatag ttattttttt gattggatat caattttata ttttattata gttgtagaaa
     5161 aaataaataa caatattttg aactaagtat aaataagttt tatatatttt agtttacaaa
     5221 agaactaaaa attttgtacc ttaatacaaa aaataaaacg tatcttaaaa taatttagta
     5281 ctaaatcatt ttaagatacg ttttatttcg tacaagctaa atcattttat catctttatc
     5341 attgtgatgt tttttatgat ggaaatcata attatgattt tcaagatttt tatttactct
     5401 atttccaatc ttccatgaca aaaacaccaa taatataaat gatgctatct ttaaaggact
     5461 tgttataaaa ccttctatat cacttgccac gtaacttata actataaaca ttacaaattt
     5521 tcctgccaac attgctggca caaaatcttt taaatctttt ttaccaaaag ctgatgcgat
     5581 agtaactaaa caaccgggca taaatggaca ggcatacgct ataaataata acttaaatcc
     5641 ctgtttatcc atccaactta ttgctttttc agttttacta tttctcaact tattaaaaag
     5701 cttactatca ttaaatctgc ttattaataa gaataatgaa gttgtcccaa gtcctgagcc
     5761 aatccatgat aacaaacatc caataaataa tccttgtatt gcagcatttg ctgtaactat
     5821 agcaacaagt ggtaaggctg gtataaagct ttctataaat gcacttaata agccgattat
     5881 tgtggctaac acccaataat tttgagtcaa atcaagtagt tcttgtatat actgcatatt
     5941 gcttttcccc ttttttaact ttaactatta ttatatactc tatatgttta ttatatacca
     6001 atctcaaaaa atgaatctaa tattaaaaag taaataatct atataagttt acagttaagg
     6061 tataattatt ttattatata ttataacaaa aaaggtgatg tttcaaaata aaaatctgaa
     6121 acatcacctt tttataactt aaattgtcat gtaagttata aaaaatatat attagacata
     6181 atattactaa aaatgtctag tcagagtaaa ataaattaca cagtacaaca aatattacta
     6241 tttaattgag ttacttcttt ataacattta accttcttcc tactctgctc aataattcat
     6301 tcgatatact acatgagtta tttgcaactt cagaagcaga taattcagat aaattatctc
     6361 ttcctattat tattgccgta ttaccaactt ctacattagg tatatctgta atatcaacag
     6421 caagttggtc catgcaaatt cgacccacta ttgctgctct acaaccattt attattacgt
     6481 aactttttcc acaggataaa tttctaggta taccatcggc atagccaata gataatatag
     6541 caattacgct atctctgctt gcaataaaga ctcttccata tccaaaacta tcatcttttt
     6601 gtatttttct aataagaatt acttgtgatt ttaaggataa aacaggtcgt aaatcaagtt
     6661 gcaatttagt cgtatcattt ggagaactta atatcccgta taatgcaatt ccaattctta
     6721 cataattaca cttaagattg ggataattaa aaaaaccata actactttga atatgtactt
     6781 taggaatttt tatacctttt tttaacagaa aatcaattaa cttataaaaa cattcaattt
     6841 gtttatttgt aaagtctaca tcttcatctt tcagactatc tgaaacacac aagtgtgtaa
     6901 atatgccaca tatatctaag tattttaatt taaatgcctt taaaattgac ctcacatcat
     6961 taacatcaaa acccagtctg tgcatacctg tatcaatttt aatgtgtgat tttatgttgt
     7021 atttctgttt gtttaaagag atagcatgat taaggtcaat aacactctgt gtaagattgt
     7081 atttatgaag ttcctttgcc cttattgggt tagtatatcc aagaatcaat atttctccta
     7141 gtattccata tcgacgtaaa tcaatcccct catctattgt tgctactgca aatgctttta
     7201 cacctatttg gtttgcacaa gtcgctactt caaaagcact atgaccatag gcattagctt
     7261 ttacaactgc catcaattca caatcttctg gcatagcttt ttgtatcatc tttacattat
     7321 gttccaaatt atttaaatca atttcaatcc aagctctatt ggtatttacc ttatgttctc
     7381 ctttcaagtc tttttttaga ggtttaagaa tcaatataat aattatagaa gctataaaag
     7441 atgctatact taccactata taatgaataa ggctattgtc aaccaagaca gtttgtattc
     7501 cgaatatttt agaaaacata cgaataacaa caatcatcat aggatgtata atataaataa
     7561 ttaatgatga tgttcgcaat aacctagctc gagaaccttt caaaaatgtt aaggctgtaa
     7621 ataagaaata catacatggc aataacataa tatacatgct atcatgacgc tgtaccccta
     7681 gtttatgtaa aaacattccc tcacataaca taagtaagaa tgatataaga aatccaatca
     7741 agctattttt agttgacata taattagatt tattagatat tattccacca agtatgaaaa
     7801 atacaggtgc aaaaaatata ccatttcgtg tataatctga tacttcaaaa atatacccat
     7861 ataaattctt taatagtggt attttttcta aaaaaccata ataactatca ccaaacatac
     7921 caattacata aataacaata gtgattacta gtgagtactt taaacctagt ctttttacaa
     7981 gaaagtatgc aatacttgca ccaataattg atgcaggtag ataccataaa tgatataaag
     8041 tcccatcaaa tacaatgtcc ttaataatat tgggcactaa attattcatg gaaaaatatc
     8101 cagtgtaaat atttaaggga atataaatta caatagctat cccgtaaata aataatacat
     8161 tttttagaaa agatttcagt ttattacaat tataattata tgttgaaatt agaaaaaagc
     8221 ctgatagcat aaaaaagaat ggcacagcaa tacgagcaat tatactagtt aacacaaagt
     8281 cagccatttt actatatgat aataatggag atgtatgaat tgcaattata agtaatgcag
     8341 ctataatcct aaaataatca agccctgcat aagatttatt cctaatcatt cttgttcctc
     8401 catttcttag tcctttctat ttctccaaaa tgtcacaata aaaccatcct tattgttacc
     8461 cgatatttga tatacacatt gttcaggtat agagattctc acttcttctt tagtatgaat
     8521 aggcacataa aagatttcta taattttttc tttatcttct atttttaaac aatttgtata
     8581 cgaaaatctc ttaatatatt ctatatattc ttcaagagcg agattcattt tattgataat
     8641 ctcagaatgc ggatatccca cataacgaaa atgccatggt tcttgagata ttcctgttat
     8701 ttcttctttt ttacactgat aacgctctat aaaaccataa tgtagtgctg ccttacgaaa
     8761 ctcattacaa ataccattat aaggaaattc agggcagata aaatctataa cttctttctt
     8821 taaccctaaa tcaatagcaa gccctgtctg atgttcactg tgatttggaa gtgctacaaa
     8881 ctttttagta aagtcttttc cattgtcacg cagagaactt gaatatatat cttgttgttc
     8941 ctctgcagta cgatatccac ttacaggtac tatttcattt attccacata tactttttaa
     9001 aatattttgt aaaatgtttg tagcttcacg tttcataaga atacttggaa accttgtatc
     9061 tacaggaatt aaatctatat cttcagatgt tttaactggt aaaaaagcat ttactagaat
     9121 tagattccca tcataaactt tatctttagt caataagata gtatttgtca ttttatcttc
     9181 tttattttta tctaataaaa tattattcat cattttatgt acacctcaat tatcttgtcc
     9241 aatatttctt caaaagacaa tccaatacct ttcatcatat ttggataacg actatgagaa
     9301 gtaaagccag gaattgtatt gacttcatta aaaataattt caccagatgg agttaaaaac
     9361 atatctactc tagcaaatcc agaacatcca agagttctat aaataatttt tgctgtctct
     9421 tgtattcttt tctcacaatc caaatcaatt ctagctggca tatgaatttt tgaacttttc
     9481 aatgtatatt tttctgtata atcaaaaaat ccttttgaca attctatttc atctactctt
     9541 ccaacaagta agtcatcgtt tcctaaaact gcacacccta cctcaaaacc atcaatattt
     9601 tcctcaatta ttacttcatt atcatgttca aaagccaagt ctactgctac ttgtaactct
     9661 tcttttttat aaattttact aataccaaat gatgaacctg cacgcacagg ttttataaat
     9721 aatggataag ttaagtcttt tgacagttca gccacattaa atttatctga gtattttagt
     9781 aatattgatt ttggtacttt aataccttct gaatgaacaa gtttatgtga tttgtctttg
     9841 tccatacata atgctgaaga aagagtacca catcctataa cctgtatacc tgccaattca
     9901 aataacccct gcactgttcc atcttctcca ttttttccat gcagtacagg aaacacaaaa
     9961 tcaagtttag tgtatttaat atcattttca catgattcaa tagcactatt tggaatatga
    10021 ttctgagaaa cagcatcagg aattaaaaat tcaataatac catttttgac acggttctgc
    10081 gaaacaataa ctggaattat atcagtatcc ctttcaaacc aagtgttatt ctggatatta
    10141 ttataatctc caatataatg ataccaatta ccctcccttg taattccaat tggtataata
    10201 tcatattttt ccgtattcaa attctcaagt actgcaaaag ctgattgtaa tgatacatca
    10261 tattctgttg agtttccacc aaatataact gcaatttttt tattattcat ctttgattct
    10321 cctttgttga tttatttatt tttatacaat ctacagaaac atgttaaaat actttttcta
    10381 tttcaaatcc aatcaacctt cacttacaat tgcatctgtt ccacataata tattttcaca
    10441 tttttctatt gatataatta ctgtatcaaa ataaaagcac tcttgttttg atacagtaat
    10501 tatatcaata acaagagtgc tctttattta tttatcctaa agaatttcct atttttttat
    10561 acttaaattc ttacaaattt cctacaatcg gaataataac cttaaagata attatattgt
    10621 tctcgctatg agctgttatt gtcccgttat gaagctccac tatttcttta gctatagcaa
    10681 gtcccaaccc agctctacca ctgtttgaat tacgagatgt atccaacctg taaaattgtt
    10741 caaaaatacg ctcaagtttt tcctctggaa tcgtattgct atgatttata aatttaagct
    10801 ttatataatc atcctcctgt acagctgtta tttcaatagt agtatcttca aaactataat
    10861 aaacagcatt tcgtaataag ttatcaaata cacgttgcat tttattaaca tcacacctta
    10921 tcatcatatt tggtgtaaca ttcagtgtat attttaaatt cttactcatc aaagtaggtt
    10981 taaactcata tgttagttgt tcaagcatac gagttaagct tactgtactg tattcaagtg
    11041 atatatttga taaattaaac cttgtaattt caaagaattc attaattaaa tcttcaagac
    11101 gctctgcctt atccaacgaa atagataaat atttttttcg cagttcttca gaaatctgac
    11161 tttcatctcg aagtagtgtc agatacccta taactgatgt aagtggtgtt tttaaatcat
    11221 gtgcaagata aacaataagt tcattcttac gttgttctgc catttgtgtc tcaaatttac
    11281 gttgttcaag tatatgttta attgagttta ttttcttttc tattgccaat aactctggag
    11341 ataaagccac ttctccaaca tcttctttga taagagagtc aataccttga tttatttcac
    11401 taaaatattt tgtaaaccaa tttaaataaa cccggaatat tataaaaaac acaatcacca
    11461 tagctagtat catgaagagc tccatgctat tacggaaaat ctgctgatat aaattcaatg
    11521 cagctttata ttccatacga aaagcatttt caaagaatga tactatccag atagaaaact
    11581 taccatacaa tataaaagaa tataaaatcc atatgaaaat aatggctata aatgccatta
    11641 aagctgttct cataaaaatt ttatttctca cttgagtaaa gtcaggattg actttaatat
    11701 ttttactttt caattgtata acctactccc catacagttt ttataaattt cgggttttta
    11761 gaaggttcat gaattttttc acgtaatcgc cctatatgag ccataactgt gttattatta
    11821 tcaagatatt tttctcccca tacagcttca aacaattctt cagaaggaac cacttttcct
    11881 tgatgttcac ataaatacca aagaatagaa aattcaatcg gtgttaatgc tacttctttc
    11941 ccaaataaac tacatttatg tgtttcttta ttgataatca atcctttaat atcatactca
    12001 ttaacaatta tcgactgttg ttcataagaa ttattatatc gcatatatct tcttaattgt
    12061 gtctttactc ttgcaacaac ttccaaaggg ttaaatggct ttgttatata atcatcagca
    12121 cctatggtta aacccattat cttgtctata tcttcaattt tagctgttaa cataatgata
    12181 ggataatagt atttttctcg aattttctgg caaatatgaa atccatcaat atcaggaagc
    12241 ataatatcta aaatagccat atccaaattt ttagattcaa tacattctag agcttcctta
    12301 cctgtataaa acttgtatac tgtaaaacct tcatttttta aatatacttc aattaaatct
    12361 gctatttctt tttcatcatc cacaatcaaa atattttcgt tcatagatta tttttcactc
    12421 tccttattat cgaggtcatc atttataata ttggtaaatt tacatatcaa ataattacaa
    12481 gcatatttgt aactaatttt tataagaaaa gaactactac atcagaatgt gttgatgcta
    12541 ctattggaac acaaaaactg agattaattg ttattataaa aactccccta acacgtcctt
    12601 attatcgcat aacaggtatt aactttcaat aaaaatatct atcagtcaaa cacaattcaa
    12661 tttattatac cagagacaat ccatataaaa aacaattaaa cctatgtatg taaccacaga
    12721 cattactaaa atttccatgt tattttatat aaaaattcaa aaaaggagtt gaatttaaaa
    12781 tttgattaga aaaaaatcaa ttttgagaca actccttttt attttaatgt atttaaatta
    12841 tagtatatca gccttcataa tatcctcaaa atcacctttt tcagatatta atcttatcaa
    12901 tttaacagta cagcttaaat catttataag tatagctcca actaatgaac catctttcat
    12961 aaataatttt ttatatattc tttgaccttc atcttttgat gaaatttctt tatcaaaatt
    13021 ttgtatatta ccaatactaa atacttttac attcattgca tctaatgaaa ctggatatac
    13081 ttcactttca aatgtcaaat tatctccact tgcatttgcc cctgctattt ttcccatttc
    13141 tactgaagat ggccatattg ctaaactaat atttcccact tgtgcaacat ctccacaagc
    13201 atatatatct ttaacatttg tctgtaatgt cttatctaca acaatacctc tatcaatttt
    13261 tacagatgta tcctgaacca tttgagtgtt tgctcttaca ccaattgaaa atactaccat
    13321 atcacagtcg acactatctc catctttaaa tacaactttt tgagcttttc catcaccttc
    13381 aataccatca actgctacac ccaatcttac atctattcct gtatctctta cacattgttc
    13441 taatatttta ctaccttctt tatcaagttg ttttgatagt atgctatcca tggcttcaac
    13501 tactactact tctaatcctt ttagtctaaa ttcccatgca gcttcaagtc ccaataaacc
    13561 gccaccaatc actacaactt tcttagattt ttctagtgct tcttttacac tatataaatc
    13621 ttttatattt ctaagagtaa acacattttc taaatcatgg cccttaattg gtggtataaa
    13681 gtttctactt cctgtagcta ttacaagctt atcaaattta atagctccat cattcactat
    13741 aatagtttta ttcacttcat ctaatttatc tacattcgtt cctaatatta ctactatatt
    13801 atttttatca taccaatctt tatcttccat gtagaaatct gaaccaagtt cttcatttat
    13861 accatcagaa agtgcaggtc tatagtaagt caactcatct tcattagaaa tcatagttat
    13921 cttacaagtt ttatttcttt ttcttatagc atctgctgca taaaaaccag ctgctccatt
    13981 tcctactatt acgaaatatt catcagtatc ttttctaaaa gtaacttcat cttcttcaac
    14041 ttctataaat tggtcttcct tagctccaca tacagggcat acactaggag cactatcacc
    14101 ttcaaaaact tcattacaaa ttacacattt aaatttctta gttttctttt tacttccaaa
    14161 agtagaattt aacttatcta ggaatttacc tgtataagaa tataatttat caatctcttc
    14221 atttgatggt ttaaagttaa cagcaaaagg ttttactgct gtcattctaa gctgacttat
    14281 tctctccata gcattttcta taccttctcc actccatcca tatgaaccaa atactgaagc
    14341 aacttttcca ccatgaagaa ctgggtttaa cgatattaat atatcccata taggctttaa
    14401 cgcatctcct aatattgttg gtgttccaaa taatacacct tgtgatactg ctatttttgc
    14461 caaaatatct tcttgcttat gctctattac atcaaataaa tttactttat agttgctatt
    14521 tttttcaata tatgctttta tagcttctgc cataatcttt gtatatccat gtgcagaaac
    14581 ataacaaatt gtaacttctt tttctaactt tatttgttca tttacactcc agttgtaata
    14641 aaggtctata atttttctag gattttcagt taatactgga ccatgacctg gacatacagt
    14701 atctatatct aaatctttta tcttttctat agcagttacc atagatggtt taaatggacc
    14761 cattatacaa tcataatagt atcttaaagc atccaagtaa tccttttctt cattttcatc
    14821 aagtgtattt actatcttat cattactata gtgactacca aatgaatcac atgttacaag
    14881 agttttatcc tcttttatat atgtgtatat ggtatctggc caatgaagca taggtacaga
    14941 gaaaaattct aaagttttgt ccccaattga taatgtatct ccatctgtaa ctgcaacata
    15001 ttcaaagtct ttgtttacga tttccttaag atatttaatt gctgtttctg aagctactac
    15061 ttttgcattt ttagctaaat ccaaaagctt ttcaacgctt cctgcatggt ctggttcagt
    15121 atgacttaca acaatatagt ctattttttc aaaatctata tttaaatcat ttaatctttc
    15181 aatataattg tcaaaatgct tatctttaac tgtctcaaat aaaactgttt tttctgttcc
    15241 ttttaataca tatgaattat aagtagttcc ataaggtgta tacataatta tgtcaaatac
    15301 tcttaaatca gggtcaagcg aacctaccca atataaattt tcttttattt ttagtgattt
    15361 caatgacata ccgtactcct cctaaaatag ttatttagtc tctttatacc ctgcttgtta
    15421 tattttaaac aaaatttatt caaaaaagaa attattttaa agatagttta aatcttctaa
    15481 tcttcttttt ctaccttcaa tacagttccc tttttagaat ctacttttat gtcatattca
    15541 tttttatctt tatctattct tattttataa aaagtaacat ctaaatcttt gtctaaactc
    15601 cattctttaa ctattccatt ttgttctttt aatgcaattt ccatagcttg ttgaggtgtc
    15661 attatgtcat ttaaatctaa tttttctttt ctagcaactc catttaaatc ttctgaatcc
    15721 aacttttctg ttttatcttc taatacatct tttgtattag catcaatttt catcttatat
    15781 tcattattat catctacacc ttccacagta taaacaaatt tatttaaatc tctctcaaga
    15841 cttaaatcta ctatatcagc atccttatat ttatctttaa atatgtttat tgcatctgtg
    15901 tatggcactt taaaatcttg ttccattaaa ttttcattat ttttttcatc ttttaaagcg
    15961 ttatttgatg catttgcctc cttttttgta ttatcttgtg cttgtcctga atccttagtt
    16021 ggactcccac tacatgcagt taataaaccc gctataagca tacttaccac taataaatac
    16081 gtttttttac acataaaaac cctccttaaa tatattaatt tattctatat tatgccctct
    16141 aaacccttta tttacccttt tatattaaaa attcctccta ttttatcaat tttaaaactt
    16201 tatatttact aaaattaaag ttacataaaa attatactgt ctcaaaatgg aaacaagcct
    16261 ctttttcatt gttgaaataa taaaaatata ttttatttcg tacaaaaaga gtgcctcatg
    16321 aactaaaaag ttcattttga gacactccca tttttttata tttttactta caaaataaca
    16381 gtacttatta attttttaca aaccttataa gtatatctat atgatattct taaatttaaa
    16441 cactatatta aaaaattaaa ttttgtttaa ttcttttacc gttaaatcca ttttaggata
    16501 tggaatcgta attccttctt cgtcaaacct aactttaacc tcttctagta aatcaagata
    16561 aattgtccaa taatcttctg tcttgcacca aactctagtt gcaaatttaa tagcattatc
    16621 tccatgttct actactccta taaatggttc tggttcattt attattaatt tatgattatt
    16681 aacaatatct ttaagtactc ttctaacatg tagtatatca tcttcatacc ctacactaaa
    16741 agtcaaatca actcgtctag tattctttgc tgaatagttt attagactat catttgatac
    16801 agaaccatta ggtataagta tctgtttatt atctggagta accaattgtg tataaaaaag
    16861 acctatttgt tcaaccgttc ctccatgacc tgctgcttct acgtaatctc ctactttaaa
    16921 aggtctaatc aatagtataa taaatcctcc agcaaaattt gatagactac cttgtaaagc
    16981 caaacctatt gctacccctg ctgatgctat cacagcagca aaactggaca aactcattcc
    17041 ccaatattcc agaacaatga ttataacggc aactttaagt acactcaaaa gaagagactt
    17101 taaaaacttt cttaaagtta aatcaatatc tcttttacta agtaataaca taacatggtt
    17161 tacaaatttt tttattattt taaatccaat agaaagtata agtatgccta ttataagttt
    17221 tactccgctt gtagtagccc attctagcag actttccatt atacctccca tatccatctt
    17281 attaatatct aaattattta aatctaactt gcttatatca tctattttcg tcgtatccat
    17341 aaaaaactcc ttaccttata ttttagaacc aatattatag tttaaagttc ttattttcta
    17401 tttaatataa tacaatacca tttgtacata gtaaacaaga tttatattcc aattatacat
    17461 attaggaaaa tgcaaactat ttgttatata tatgcaaaat ataatactaa attaaactat
    17521 taataaaaca ctatatagcc ttaaaatcat cattttttac ccctatatga gatgatacta
    17581 tagttataat acttagtact ataaatacta caaaaccttt tttatatcca tcaactatat
    17641 tatatgaatt tacttgtaaa gagatagcta taatcaaatt aataattcca cttcctataa
    17701 attcaaaagt attaataaca ctagttgcca gtccagaata aatttcttta tttttatact
    17761 tggcatcatt aaaaacctgt agatgtgaca taactataca tcccataata aagaatgata
    17821 ttggtaaaac tataattggt ggttttacct ggtatacaac tacaattaca atccatataa
    17881 acaaatctat cattgctcca aattttatta tattaaattt gctagacctt attttagcaa
    17941 atacaaaatc cataattatt gaaccaaata taaatccata tgtaaaaaat gatactataa
    18001 acgcagaaaa actcttactc acaccataaa cgtccattat gtatcttact ccccacaagc
    18061 ttataaatgc acttgtaaga ccaacaaaag agaacattat cattgaatta taccatgttg
    18121 atttgttctt tataacagac ttaataccat caacaatatt aaccttctct gacttttcat
    18181 agggttcagt atctacattg aatccatact cttttggtgt atctctaaca ataatataca
    18241 taaaacatcc aattacaaca ccaattatac cgattaataa aaatgaattt ctccaaccaa
    18301 caagttcaga caaaaacaca agtggaaaag tagcaagaac tccacctaaa tttcctacaa
    18361 atgaaaattt agctgttgca gaagcaaatt ctgacttatt aaaccatctc ccttgaattt
    18421 ttaaaataca aagtaatatt actgatgttc ctgcccctac aattacacga gaaatatatg
    18481 ctaattctat actttgaatc aaaccaaata aaattgaccc taaacttgcc attattattc
    18541 caagactgct aatttttctt gctccatatt tatcaatcaa aattcctgca ggtatttgca
    18601 taaatgcata tgcatatagg caaaaggatg ctatatttga aatttgtata gaattaaaac
    18661 ctaactcatt tgctaaattg tctgaaacaa cagcagttga catccttaaa aataatacta
    18721 aaacaaatga aaatgtaatt ataccccaaa cactccactt agcctttgta taacttttca
    18781 tatcttctcc ctcaaatcca tatattttta aattttcttg atataactta tataaagtgt
    18841 atagttattc ttacaaattt ttacacgcaa tatcattata caacatatgt atatatttaa
    18901 ctagaaataa aacactattc atattatata catgaattaa ctacataaat aataagagcc
    18961 atcttttaac attactcaaa agatagctcc atataaaaac atttattcta aatttaattt
    19021 tttatctaat ataaattttg tagcgaaaaa taacgctatt gcaactacaa gactaccaag
    19081 tattgcaagc ataaaactag gactctgata aagatgatat accatatttg tatcttcatt
    19141 tggtaagatg cttccaactg catctccaac aattgatatt actatattta tagctataaa
    19201 agaagcaaat gccactatgt ttctatgttt attgaatttt ggcaattgtc ccattgagat
    19261 tgatgtatat aataacaaaa taaatgatga atatgaaata aacattacta ctactaataa
    19321 taaaatatct aaaaagtttt cagaaatcca tttaaaacta gtgttaaaca aatctaaaat
    19381 ttcaggtagt aaaattccag aagtaccaaa aagcataaga actccaaaag tgaatactgc
    19441 aactataaaa ctaagtacag cgtatataag tgctgttata catttagata atataagtga
    19501 actagtgcta acaggaagag taaacattaa ataaccttca tcccccaaaa gattttttct
    19561 aaacctttga attgtaacta caatagttaa aactcctaat gccataaaaa gacttgttaa
    19621 tactagtatt cctattccct gtacctgaag tatatttgta ttcataaata ttccattaaa
    19681 tacagctact atcaatatgg ctatatataa cggtataaat attcttccac ttgcttttaa
    19741 ctcatatttt aataatttac ctaacattta aattcctccc taaataatgc atctatagat
    19801 tttccttttt cttctcttat tgcttcaacg tctccttgta acactatttc tccttttgat
    19861 atgaatatta tctcatcaca tatgttttct atttcactta ttaagtgggt agcaatcagt
    19921 aaagtactat cttcagaata attttttaat atagtcttaa gtatatatga ccttgcagct
    19981 gggtcaacac cacctatagg ttcatccaat atatatatgc tcgcatttct tgacattact
    20041 aaaataagtt gaactttttc tttagttcct ttagacattg tctttagctt ttcatttaca
    20101 tcaatatcta agcttttaat catctcattt gctcttctaa catcaaaatc actataaaaa
    20161 tcatagaaaa acttaagtaa atctgatacc ttcatccaat catttaaata agttctttct
    20221 ggtaaatatg aaactatttt tttagtatct atggatggtt tcattccatt tatcataatt
    20281 tcaccattat caggctgtaa taatccattc attagcttaa tcattgtact tttgccactt
    20341 ccattaggtc ctaacagccc gactattttt ccctttggta tatttaaatc aatatttttt
    20401 aaaacattct tagttccata actcttgttt acacctttaa attcaactat tccatcttga
    20461 gaaacattgc ttatattttt atccatttta taattctcct ttcagatttt ctgtaataag
    20521 ttttagcatt tcatctctat catatccaag cttttctaag gttactttta atgtatttat
    20581 ctccacatta gctatttcct ctttcatagc ttttattttt tccttatcat ctgttacaaa
    20641 tcttccttta gttctctgac tataaactaa accttgtctt tcaagttctg ttagagcctt
    20701 ttgcattgta tttggattaa cttccgcatc ttctgctagt gctctcactg tttctaattt
    20761 agatcctatt ttaatttccc ctgaaattat ttttaacttt aaatgttcta ctaattgtat
    20821 atatataggt ttgttattat caagttccca ttccatgttc atgcctcctt attatattat
    20881 tgtattaagt tgttaataca ataatataat aatacaaagt atatgtcaat tactttttag
    20941 taaaagtata aaaaatttta caaaaaaaat gaacactaca ttagagtcca tttttaacac
    21001 aatttattaa attctttata ttacagatta attaccctct taccaagtta gatacaatta
    21061 aatgttcaaa ttcttcaatt ggcattggct ttccaaacaa atatccttgt gccatatcac
    21121 actttacttc ttttaaaagt ttaaactgtt cttctgtctc aactccttct gatatgactt
    21181 tcatacccaa ctcttttgcc atttctatta ctttagtaac aattattttg cttcttttac
    21241 tattataagt ttccataata aatttttggt caagttttaa gatatccata ggaatttctt
    21301 ttaacatatt taatgatgag taccctgaac caaaatcatc catagatatt aaaaatccta
    21361 ttttttttaa attattcatt atatcaatta aaatatcaaa attatcaaat atgatactct
    21421 ccgttaattc aagttctata tactttgcag gtatattgta tttatcaatt atatttttgt
    21481 atgtttctat aaaattattg caatataaat gtatccttga aacattaact gaaacagtaa
    21541 ctggatttat tcctgcatca atccattttc taaggtatat acatacttct tcaagcacat
    21601 ataaatctaa atttataaca aaaccgtttc tttcaaaaaa tgggataaat tcatttggag
    21661 gtattagccc tttatcagga cgtttccacc taacaagtgc ttctgaacct tgtatctctc
    21721 ctgtattaag ttctatctta ggttgtaagt atataataaa ttctttattt tctagacttg
    21781 aaaacattgt attttccatc tctttttctt ttatctcttg gtcatgaatc ttattatcat
    21841 aaaatgcata tgaactttta tgtcctccct ttgccatttt atgaggtata ttagccctat
    21901 ctatgattga gttgatatct ctatcttctg gaagtataaa atagattcca caacttaaaa
    21961 ctaacttata tttttcttct tgattattat taaacaagtc taattcttca aatatagttt
    22021 ttaacctctt tgttatatct tcttcttcta tatatttcat aattataata aaattatccg
    22081 cagatattct tgcaaacatt tcctcttcaa atatattgct cttaataatc tttgttaaat
    22141 tacgcaaaat ttcattccca acttcatatc caaataaatc atttatatat ttaaatttat
    22201 caatatctat atataataaa gcatacttta cttgagtatt tttacatagt attttgttag
    22261 catcaattac aaatttatct atagtatttg ctcctgtaag attatctgta tatgctatcc
    22321 tttgcaattt aattttaaaa tttaatttat catatattat atagaatata agtattacac
    22381 atattatagc cataataagt attatttgta ttgcatgttt gctaataagc tgattattag
    22441 acacaataga ctcaaagtta tttatggagt tgtttttata gagtttgcat aaagaataga
    22501 ctggtgttgt gtatgtaatt tgtaaaatta tacaaataaa taaaaaaaac atcaattttt
    22561 tttgtaaatt cctcataaaa atttcctccc attaaaacac ctctatttta ttagcaaata
    22621 agtacatctc aaaagttt
//
LOCUS       DAYAOY010000063        22309 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYAOY010000063 DAYAOY010000000
VERSION     DAYAOY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.063"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            395..3049
                     /gene="adhE"
                     /locus_tag="ACGI6B_002508"
                     /gene_synonym="adhC"
     CDS             395..3049
                     /gene="adhE"
                     /locus_tag="ACGI6B_002508"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFG9751945.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3325..5544
                     /locus_tag="ACGI6B_002509"
     CDS             3325..5544
                     /locus_tag="ACGI6B_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9751946.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5798..6460
                     /locus_tag="ACGI6B_002510"
     CDS             5798..6460
                     /locus_tag="ACGI6B_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9751947.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6831..8240
                     /locus_tag="ACGI6B_002511"
     CDS             6831..8240
                     /locus_tag="ACGI6B_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFG9751948.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8355..8630
                     /locus_tag="ACGI6B_002512"
     CDS             8355..8630
                     /locus_tag="ACGI6B_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HFG9751949.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            9260..9571
                     /locus_tag="ACGI6B_002513"
     CDS             9260..9571
                     /locus_tag="ACGI6B_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751950.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            9573..11498
                     /locus_tag="ACGI6B_002514"
     CDS             9573..11498
                     /locus_tag="ACGI6B_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFG9751951.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            11501..11992
                     /locus_tag="ACGI6B_002515"
     CDS             11501..11992
                     /locus_tag="ACGI6B_002515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFG9751952.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            12044..12607
                     /locus_tag="ACGI6B_002516"
     CDS             12044..12607
                     /locus_tag="ACGI6B_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFG9751953.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            12622..13599
                     /locus_tag="ACGI6B_002517"
     CDS             12622..13599
                     /locus_tag="ACGI6B_002517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HFG9751954.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            13592..13909
                     /locus_tag="ACGI6B_002518"
     CDS             13592..13909
                     /locus_tag="ACGI6B_002518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFG9751955.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            14026..15804
                     /locus_tag="ACGI6B_002519"
     CDS             14026..15804
                     /locus_tag="ACGI6B_002519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFG9751956.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            15801..17174
                     /locus_tag="ACGI6B_002520"
     CDS             15801..17174
                     /locus_tag="ACGI6B_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFG9751957.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            17180..17848
                     /locus_tag="ACGI6B_002521"
     CDS             17180..17848
                     /locus_tag="ACGI6B_002521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFG9751958.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            18125..19174
                     /locus_tag="ACGI6B_002522"
     CDS             18125..19174
                     /locus_tag="ACGI6B_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9751959.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            19270..20259
                     /locus_tag="ACGI6B_002523"
     CDS             19270..20259
                     /locus_tag="ACGI6B_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9751960.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            20264..21982
                     /locus_tag="ACGI6B_002524"
     CDS             20264..21982
                     /locus_tag="ACGI6B_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFG9751961.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(22064..>22309)
                     /locus_tag="ACGI6B_002525"
     CDS             complement(22064..>22309)
                     /locus_tag="ACGI6B_002525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9751962.1"
                     /translation="RIGGDEYIIILKNLSHKEINNIINNINLKIVEENSTNELKLSMS
                     IGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
BASE COUNT         8613 a         2611 c         4085 g         7000 t
ORIGIN      
        1 ttgtaaatgt ttgtacattt aactatattt actttaactt gttaattaag ttttaatttt
       61 cattttcaag gatttttatt tttaaacttg atagagtatc atattaaaaa ttctttttat
      121 aaagttttat ttgcaaaaaa tacaaaaata taaacgttaa aaaaataacg ttatccttaa
      181 gtttttagat gatttcatta gtgatttgcg taaaaaataa agtgatatct agttaaatta
      241 agtatttgta aagctagata ttattagtag aaatgatgtt atattagata aaaaataaaa
      301 tgttaaaaaa ataacttgaa aataggaaaa gtaaatgcta taatgttaat aaattaacat
      361 aataaataaa aaactatctc aggaggttgt taaaatggct cataaagaac aagtaataat
      421 agaaaataaa aaagaaaatg aagttatgtc agttggttta gatactgtaa ataacgttga
      481 aagcttagtt aaaaaactag caaaaatcag agaagctcaa aaaatatttg ctacatatag
      541 tcaagagcag gtagataaaa tttttttagc agcatctttg gcagctaata agcaaagagt
      601 tcctctagct ataatggcac aaaaagaaac tggtatggga attgctgaag ataaggttat
      661 aaagaatcat tatgcctcag agtatatata taatgcatat aaagaaacta aaacttgtgg
      721 tgttatagag aaagacgaag catttggtat gactaaaatt gctgaaccaa taggtgttat
      781 tgcagcagtt gtacctacaa ctaatccaac ttctactgct atctttaaag cacttttagc
      841 tttaaaaact agaaatggaa taattttctc tccacatcca agagcaaaaa attctactat
      901 agaggctgca aaagttgttc ttgaggctgc tgttttagct ggagctcctg aaggtataat
      961 aggatggata gatgagccat ctttagaatt aactacaact gttatgaaag aagtggattt
     1021 gacccttgct acaggaggtc ctggaatggt taaatcagca tattcatctg gaaaaccagc
     1081 tattggagtt ggtgcaggta atactccagc aataattgat gatagtgcag atataaaaac
     1141 tgctgtaaac tcaattcttg tatctaagac ttttgacaat ggtatgatat gtgcttctga
     1201 acaatcggtt atagtattag aaaatatata caatgaagtc aaaaaagaat ttaaagagcg
     1261 tggtgcttat ttattagata aagatgaaac agaaaaaata agaaatataa ttttagtaaa
     1321 tggaggatta aattctaaaa tagttgggca aacagcttgt actatagcaa aacttgcagg
     1381 ctttgaagtc ccagtagata ctaaggtttt aataggtgaa gtagaatcag ttgaaataga
     1441 agaagctttt gctcatgaga aactttcacc agtacttgca atgtacaagg caagtgattt
     1501 tgatgatgca gttagaaaag cagaaaaatt agtagaagat ggtggatttg ggcatacatc
     1561 ttcattatat attgatgatg taaatcaaag agaaaaactt gataagttta cttcagccat
     1621 gaaaacttgt agaattttaa ttaatactcc ttcttctcaa ggtggtatag gggacttata
     1681 taattttaaa ctagctcctt ctcttacttt aggatgtggg tcttggggtg gaaattcagt
     1741 atctgaaaat gtaggagtta agcatttact taatattaaa acagtagctg agaggagaga
     1801 aaatatgctt tggtttagag caccagaaaa agtttatttc aaaaaaggtt gtttaggagt
     1861 agcattaaaa gaacttaaag atgtaatgaa taagaagaga gcttttattg taacagatac
     1921 attcctttac aataatggat atactaaagc agttactgat ttattagatg aaatgaatat
     1981 taagtacact acattctttg aagtagaacc agacccaact ttggaatgtg ctaagatagg
     2041 cgctaaagct atgagagaat ttaatccaga tgtaataata tctataggtg gaggaagtgc
     2101 aatggatgca ggaaagataa tgtggacatt gtatgagcat ccagatgtag atttccaaga
     2161 cctagctatg agatttatgg atataagaaa aagggtatat acattcccta aaatgggtga
     2221 aaaagctaat tttgtagcta taccaacatc agcaggtact ggttcagaag taacaccatt
     2281 tgctgttata actgaccaag atacaggagt aaaatatcca ttagcagatt atgaactgat
     2341 gccaaatatg gctatagttg attctgatat gatgatgaat atgccaaaaa gcttaacatc
     2401 tgcttctggt atagatgctt taactcatgc attagaagca tatgtatcta tgcttgcaac
     2461 cgattataca aatggattag ctttacaagc tattaagagt atatttgaat atcttccacg
     2521 tgcatatgat aatggtgcaa aagacccaga agctagagaa aaaatggcaa atgcttctac
     2581 aatggcaggt atggcttttg caaatgcatt tttgggtgtt tgtcactcta tggctcataa
     2641 attaggtgct ttccaccatg taccacatgg tgttgcaaat gctcttttga taacagaagt
     2701 tatgaaattt aattcttcag atgcaccaaa gaaaatggga gcattctctc aatataaata
     2761 tccagaagca ttaaagagat atgctgaaat agcttcattt ttaggattaa aaggaaattc
     2821 tgatgaagag aaattccaaa gtttattggt agctattgaa gatttaaaac ttaaagttgg
     2881 tattcctaaa tctataaaag agtttggcgt tgaagaatct aagtttatgg attcaataga
     2941 tgaaatggta attcaagcat ttgatgacca atgtacagga gcaaatccaa gatatccact
     3001 tatgaatgaa ataaaagata tgtatttaaa ttcatattat ggaagataag tttgctataa
     3061 aatagataaa ataaaaactc ttatacataa aacaaagttt tattgtataa gagttttttt
     3121 gtttgagaaa atggatagtt tttattcttt aatttaaaat tcaattaaat agtgttatta
     3181 taataataaa agaatgtttt tatggttatt atgttgattt tatgtgtaac atttaagaat
     3241 agttatatac aatataaatg atgaattggt ataaaaagga tatattatct atatgaagta
     3301 ttgactaatt aggggaggag tcgaatgaca gggagaaata aaagttttac attaattaca
     3361 attctttttt taacatcatt atttttgaca atggggtata tctatataga agatacaaaa
     3421 catcttctta tgtcagaggc aaaaatacat ataaaagaag ttgcagtaca aggctctcaa
     3481 ctagcacaaa gacaaattga aaaagattta gatgttttaa atattttttc taactattat
     3541 gcttcaaatc cagatatacc taatgaggaa aaaatgaaaa atcttttaga tgaaatggaa
     3601 aatcaaaaat tttacactat ggcaatagtt gatataaatg gtaatgctga aaataccaaa
     3661 ggtgataagt tttcagtgaa agatagagaa tttttcaaaa actcaattaa aggtaagaag
     3721 tatgtttcaa gtccatatgt tgatgaagta aataagtcta taaagaagat tgctatttca
     3781 gtaccattat taaataatga taaagtagtt ggagttttat attgtactta caatatcaat
     3841 actctgatga aacttattaa tatatctttt tatgaaaata atagtatatc ttatgttgta
     3901 aaaaataatg gtacaattat ccttcatcca caaggggata gcctatcaaa aaatatttat
     3961 aaattgttaa aacaggataa tgatattcaa gaagtaaata gattaaaaaa agaattgcaa
     4021 gaaaataaaa caggtgcaac tgtattaaat atgttggaag aaagaagata tttaggatat
     4081 gccactatgg ataatgggaa tagtgaaaat aattatataa aagactggaa tttaattttt
     4141 tcaattccag aaactgttgt atttagtaat tcaaaacaaa taatcaacag agctgtatat
     4201 gcagtgttat ctattgtatt aatatttatt gcaattattt tttatataat ttatataaaa
     4261 aaatctaatg aaaaagaaat attaagtttg gcttatgaag ataaagttac atatatagga
     4321 aatcaaaata aattttatag agaatgtagt aagtatcttt tagataaacc atctttaaat
     4381 tatattatag tatattttga tattaataac tttaagatga ttaatgatac ttttggatat
     4441 gaatttggtg acaatttatt aattacaata gcaaaagctt taaaagaaga attaacagaa
     4501 ggtgaggtat atgcacgact ttcaagtgat tattttgcaa ttttctgtga ttataaaaat
     4561 ggaagaaata aaattataag aaaacttgat aatatacgaa gtaatattga aagtaatttg
     4621 agtatagtct ttgaaatttc tctgtgtgtg ggtatttatt ttgttgaaga aggtgaagta
     4681 gatattcaaa aggctgtaaa taaagcaaat atggctaggt ctgttgctaa aggtaaaaat
     4741 ataaattatg ctatttataa tgaagatgtt aggaataagc ttagtgagga atcaatgatt
     4801 ttagatgata taaaaatagc attggtaaaa aatcaatttg aagtgtatta tcaacctaag
     4861 ttttcacttg taactggaga aatgataggg agtgaagcac taataagatg gaatcatcca
     4921 gaacatggtt ttataagtcc cgcagtattt attccaatag cagagaaaag taagttgatt
     4981 ttaaaaattg ggagatttgt atttgaaagg gtttgcaatg atttatatga atggaaaaag
     5041 caaggtaaaa agatagttcc agtttcagta aatttatcta gggtcgagtt gtatcaacca
     5101 gatattgtaa aatttatcaa taaaacaata aaaatgtata atttaagttc agatttcatt
     5161 gaaatagaga taacagaaac tgtggctatt aatgagttaa atattttaaa gaatgtttta
     5221 aatgagttga ggacgtatgg attttctatt tctatggatg actttggtac aggatattct
     5281 tctattagtt gtttaagaga tatgccaatt gatatattga agctagataa gtcttttctt
     5341 ggtggcattg aacatgatga gagaagtcgc aatatagcaa aatcaatagt gtctctagca
     5401 aaatcactag atttagttgt aattatagaa ggtgttgaga gtaaagaaca agctgagtta
     5461 atgaaacaat ttggatgtga tttagttcaa ggattttatt ttgcacgacc aatgccagct
     5521 aagaattttt tagatttact ttaaaacaaa atttgatttt tatataaagc actattatga
     5581 atgcttgatt ttaataaatc attgctaaat aattagtgtt tttttatttt atcaatgtat
     5641 tgtctaatat aaagcaaaaa ataattgtat tttataaaaa ttatattaaa aataaattta
     5701 ttttgctaat aaacatgtta ttaacaaaag aatagtacta taatgtatag atgataaaaa
     5761 ctttaataaa aatttagtgt ataggagttg tttaagtatg atagatataa gtattgatga
     5821 caaacttaag gaaagatggc ctaatgctaa gctgggttgc attcaagcaa aagtagcagt
     5881 aaataaaagc tcagaaaaat taattaatga aataaatgag ttttgtgata ctataaatca
     5941 aagtctaaaa gttgaagaca ttacgaaact agataaaatt aaagatgcaa gaaatgctta
     6001 taaagagctt ggaaaaaatc caagtagata tagaacatct tcagaagcat tggttagaag
     6061 aattattcaa gggaagggtc tttatacaat aaataatata gtagaaataa acaatttaat
     6121 atcaataaaa tcattatatc cagtaggtac atataatgta agcaaattac attcaccagt
     6181 atgttttact gtaggagatg aaggagaaca gtataagggt attgggaaag aattaataaa
     6241 tatagaaaat cttcctatat tatctgattc agtgggtaaa tttgggagtc caactagtga
     6301 ttctgaaaga gcaatgataa cagaagatgc aaatgaaata ttaatttgta tattttcttt
     6361 tagtcaagaa tctgatattg aagaatattt tggatatgct aaggaattac ttaaagaata
     6421 tgcagatgga aaagatatag aaactaaaat aattaaatag aatctatata acaacgttat
     6481 gttaagctaa aattcaatat aaaaataagt taagataaag aaactaacac aaaattagtt
     6541 tcttttttaa aatagtagtt tagtgttaca aaaatagata gatagtccca cgattattaa
     6601 aagtatggta atatattaat atagcagaga ttgtgtttaa gggattttga aaggggggat
     6661 ttataataat cattttatct aaaagataaa atggttttat agaaataact atatatttaa
     6721 aaatataaat aatgatacag taatttgggt atataaaata aggcatacat ggttttgttt
     6781 aaaaaccaat aaggtacata ggtaattaca atgtaaggaa ggatttaatt atgattgatg
     6841 gaaaagaggt aaaacaagaa agtggacaaa aaaatctaaa atttaagata gctatagtag
     6901 taggtgtttt gtttgtagct tatataggaa tggcaatctt ttttagaaat catttttact
     6961 ttggaacgac tattaatggc gtaaaggctt caggaaaaac agttgagcaa gtaaatgatt
     7021 tgttaacatc tagtactaat tcctatactt taaacttaga ggaaagaaac aataagaaag
     7081 aacaaataaa agcaaaagat attgatttaa aatttacacc agatgataaa gttcaaaaga
     7141 taaaagatag tcagagcgct attggatgga ttggtggaat atttaagtct aaagagtata
     7201 ataatatgat aactctttca tataataaag atttactaga aaaaaggttt aatgctttat
     7261 cttgttttga tagtaaaaat gttgtagaac ctaagagtgc ttatcctaag tatgatagca
     7321 ctaaaaacgc ttatgttata gtaccagagg tacttggaaa caaagtgaaa aaagatgatt
     7381 tttataagtt agtacaaaac tcaattaatt ctgggaatac ttctatttct ctggatgaaa
     7441 gtaaagttta tgaagcacca aacaacacaa aggattcaga aaagttgaaa caagctatta
     7501 agacattaga taagtatgtt ggtgtagtcg ttacatatga ttttagtgac cgtaaggaaa
     7561 ctttagatgg ttctgaaatt aataaatggt taaaagtaga ctcagaaaag aattatgaca
     7621 taacttttga taatgatgct atggtagaat atacaagagg tttaagtaaa aaatacagta
     7681 cttttggtga ctcaagacca tttaaaactg ccacaggtaa agatattaca atttcaggtg
     7741 gaatttatgg ttggttgata gataaaaaga aagaagctga agcattagtt gatgttgtaa
     7801 aagctggtaa aaatgttaca agagaaccta tttacataca aaaagctgta tctcgtaata
     7861 aagatgatat tggaaataca tatgtagaga ttagtctttc tggccaacat atgtggttct
     7921 ttaaaaatgg agaaatgctt gtatcaacag acgttgtaac aggtgattta gctagaggat
     7981 ttgctacacc atcaggtata tatcctctta attataaggc tagaaatgtt tcattaactg
     8041 gtcaaggtta cagttcacct gtaagttatt ggcttccgtt taatgggaac ataggcattc
     8101 atgatgctac ttggagaaat tcttttggtg gaagtatata taagtcttca ggttcacatg
     8161 gatgtgtaaa tacacctttt tctaaggcaa agaaaatata tgaaaatata gaaccaggta
     8221 caccaattat tttatactag taaatgtgtt gctaacagtt aagcgaagtc atcttagttt
     8281 atttaggatg gcttcctttt tatataaagc ttattgaagt ttacagaaag actacttaaa
     8341 aggagaaata cataatgaat gaaaaattaa ctaaaatttt agaagaagct aagaatgatg
     8401 atgaatttag aaaagaactt ataaaaacaa gagaacaacg tgatgttatg gattcatttt
     8461 gtaaggttgt tacatctaga ggttatgata tgacagtagg ggaacttttt tctatgggag
     8521 aagaatatac atccaatcta ctgaaaagtg taaatggagg agcagcatat cctattgaag
     8581 attggagtga ttggtacgag gattttttta taatgttgtc gaatatatag agtaactaat
     8641 gatattatct acttaagatt aatacattgc cttaaaataa aactattaga actaggtgta
     8701 agttttatca atctttaaaa atattgattt atttctaact agatataaaa aaattagatt
     8761 aaaaataagg tgacaaaaat cgatatagct tgatgattta taaaagttag ataaaatagt
     8821 gcgagatatt tctaaaaatt caaaaaaaac aataaacttc aatggttaga ggttgaaaaa
     8881 gaatataaaa aacttttata tgtaataaat taaattaaaa ttataaaaaa aataaaataa
     8941 aaatttaaaa tgtcgacata acgttaattt taaaacaaaa taacgctatt gacaaactat
     9001 atcgctttaa attataatta ttgaaaataa tggtatttaa aaaccatact catgtcatag
     9061 tctaaaagta agtcaaaaaa ggatgtaaat ctaggcaagt actattctaa aatatgtttt
     9121 attaagaaat aaacgttgaa aaatattaat tattatataa aaatgctgtt tatactttaa
     9181 caatgaggta gtaatctaat tagaataaaa aaatctaatt ggttatatac atagtaaaaa
     9241 ggtaagagag gtgatttgga tggcttttga aataataaaa agcattgttg aggcagagca
     9301 gacagcagac agtatcaaag taaaagctgt tactgatgca gagtctatca gagctgatgc
     9361 tgtaaacaaa tgtgaaagca tatttgctga tgtaaaaaaa caagcaaagc ttatggaaga
     9421 aactcttatt gagaaggcag tcaccgacag tagagcagag gttgataaaa tcttagctaa
     9481 tgctaaaagt gaatgtctga aaattgaaaa aactgctgaa gaaagaaaaa gtaaggctat
     9541 tgaagcggtt attggaaagg tagtgagata atatggcaat agttaatatg aacaaaatat
     9601 ctattgttgg cttagaagct caaaaaagcc agattttaaa gctacttatg aaccgtgggt
     9661 ttgttcagat tgatgatagt gcctttctta ctgaggagga ggaattaaaa aattatcttg
     9721 taaaagatag tgaagaatct gaagttataa tgctagagca gaaaatgtct catgtaaacc
     9781 aagcaattaa tattgtttct tcactagtga aacaaaaaaa atccctactt gcaccaaaaa
     9841 gagagtttaa caaaattgat agtgaaaaag aagggcaagt ttatgctgaa gctctggagt
     9901 taaacaattt gcacaaggag ataggtacaa taaggtctaa cataaactta cttaacaaca
     9961 ataaaagtat gttagaacca tgggtgaact ttgatattcc tcttgaaaat atggaaacta
    10021 agtatacaaa aactatactt ggaactgtgc ccataagcgc aaacattgat gctttaagag
    10081 taaaattaga agatgaaata ccagaaagtg ttattgggat tgtcaattct gacaagttaa
    10141 tgaactatgt ttatttaatt actttaaaag aaacttttga tgatgtttta gaagttttaa
    10201 aagaatttaa tttttcagtg gtttcattac caaatgaaga aggtacaccg ttacaagcta
    10261 tcaagaaata caatgaaatg ataattagac aggaaaacgt ttctaaggac aaaacagagg
    10321 aaattaaaag acatgcagat agtatacaca tgcttgaaaa cctatatgat ttttacacta
    10381 tagaaagaga ccaaaaaaag attatagaga gacttgtcaa aacaaaaaca acattctgtc
    10441 ttaatggttg gttaccaagc aaaagggcag atgaattaat taaagaaatt acagaaaagt
    10501 ttaattgttg tgttcagact gaagaaggaa gtaaagaaga aggcttccca attctgcttg
    10561 aaaacaataa attagtaaca ccttttgaaa gtgtaactaa catgtacagc tatccaagta
    10621 caaaagatat tgacccaaat acaattttaa ctttcttctt tgttgtattc tttggaatga
    10681 tgctaagtga cgccgcatat gggttaataa ttgcagtagt ctgtggattt gttgtatata
    10741 aattaaagat acaaaaaggt gaaggaaatt taattaaact tattggtatc tgtggagttt
    10801 caactactgt atggggtctt atattcgggg gtattttggg ggatttaatt cctataaaag
    10861 cattaattaa tcctttagag gatgttatga cacttatggg aatgtcgtta ctgttcggta
    10921 ttatccatat atatgtaggt ttgggaataa aagcatatac attaataaga agtggaaagg
    10981 taatagatgc aatctttgac ataggttttt ggtatttgtg tataacaggt gtatgtctac
    11041 ttataatacc ttttgttgca ggggatatag gtgtattttc agaagtagga aaatatttag
    11101 caattatagg tgctataggt cttgtactta ctcaaggaag aagctttaaa ggtattcctg
    11161 ttaagctttt taaaggattt tcaagtctct atggtataac aagttatttt gctgatatat
    11221 tatcttatac aagaattatg gcgctttgtt taacaacagg agttatagca caagttatca
    11281 accttttagg agctatagct ggtccgatac ttgcagttgt aattggtgtt gtaggacaca
    11341 caataaacct tcttataaat gcactaggtg catatgttca tacaagtagg ttacagtatg
    11401 tagaattttt caataagttt tatgaaggcg gaggagttcc ttttgttccg tttaagtata
    11461 aaacaaaata tacaagcatt aataaaaagg agatgtaaat atggaatttt taaacgcgtt
    11521 aggaaatggt caatttttgg caatatcagg agctgctata gcagcattat ttgcaggtat
    11581 gggttctgct aaaggtactt caatagcagg tcaagccgca gcaggtgttg taacagaaga
    11641 ccctagtaaa ttcggtcaac ttctagtttt acaacttctt cctggtacac aaggtctgta
    11701 tggatttatc atagctttcc ttattttatc aaaagcagga attattggtg gagatgcaat
    11761 accaacatca gctcaaggtt tacaacttct tatggcagga cttccaattg gtttagttgg
    11821 tttagtatca ggtatagcac aaggtaaagc agcagcagca ggagttggaa tagttgcaaa
    11881 aagacctgaa gaacttggta aagctataac atttgcagca atagttgaaa catatgctct
    11941 tttaggacta cttgtttctt tcttagctta caatggaatt gcaattggtt aattataaat
    12001 tataattcat aagtaaaaaa aatgaaaagg aggaagcact cctatgggta atgaacaaaa
    12061 aatgattgac agaattatag ctgatgccaa acaagaagca caagaaatat tggataaagc
    12121 taaaagtgaa gcagatttaa aagtaaattc agctaatgaa aaagctgaaa aggaaatggc
    12181 ttcctataca aagcttgcag aggcagaagc agaaaaagcg gcttctaagg aaatatcagg
    12241 agcttatatg gaggctaaaa agcaaatatt atcaaaaaaa caagagattt tagaagaggt
    12301 aattttagaa gctaaaaaca aacttttaaa tcttaaagat aatgaatatg aagagattat
    12361 actaaacatg attgaaaaat caaattgtac agatgattca gagatagtat tatctaaaaa
    12421 agataaaaaa acattaaaag atgtgctatc aaaaaaaggc attaaagtgt ctgatgaaac
    12481 tagagatatt actggaggtt tcatagttaa aaaaggtgac attgagtaca attattcatt
    12541 tgaagccata attgcagtag agcatgaata tattgaacaa attgcagcag aaattttgtt
    12601 taattaagga ggggttaaaa aatggcagaa gaaaaaactt atccctatgc cgttgcacga
    12661 ataagagtgc ttgaaaaaca gcttcttaac agacaaatgt ttattcaaat ggcagaagcc
    12721 aaaagccctg aagactcttt aagaattata gctgaggcag gttatgctga tacatcaaat
    12781 aataatatac ataattttga gaatattttg actcaagaat tatcaaagac atatgaaatt
    12841 cttaaaagtc tagctcctga agaaaaattt gttgatgtat ttctttataa aaatgactat
    12901 cacaatttaa aagtgctgat taaagaagaa atttctggtg tagatggtga gaaatattta
    12961 attgatggag gtacaattcc tcttattaag cttagagaat ctttagcaaa tcgtagtttt
    13021 agtgatttac ctaagataat gtcatcagct atattagagg catttgatgt ttataataag
    13081 acacaaaatg gccagatggt tgatattgtt ctagataaag caacctttac cagtatgaaa
    13141 gaaactgcta aagaaagcca aaacaaattt gtaatagatt atgttatgaa agtttgtgat
    13201 ttaacaaatc taaagagttt tataagagtc aaaaatatga agaaagattt tgatatgttt
    13261 atgaatgtgt ttgtttcagg tggctcactt gataaggaaa aattcttaga agctttttcc
    13321 tcagatactc cagcatcatg ttttaaatct acttcttact ctgatgtctg taaaaatggc
    13381 atggatagtg gttttactgt ctttgaaaaa ctttgtgatg actatcttat ggaatatgtt
    13441 agaggagcaa aatttaagcc tcttacatta gaaccactaa ttgcatattt atatgctaaa
    13501 gaatcagaga taaaaacaat tagaattatt ttgactagta agcttaataa tatagacgca
    13561 gatactataa aagaaaggct aagggatgct tatgtataag gtaggagtag ttggagataa
    13621 agatagcatc atgggatttt tagctcttgg aatagatatc ttcccagcct atgatagtga
    13681 cgaaattaaa aaaagtatac ataagttggt tgaagatgaa tatgcgataa tttatattac
    13741 agaacaggca tcattgcttg caaaagaatc aatagcaaaa tacaaagatt accaacttcc
    13801 tgccattata gttattccag gtattggagg aagtatggga ttaggtatga atgaagtaag
    13861 agaatctagt aaacgtgcta taggtgcaga tatattattt aaagaataga tgaaagttga
    13921 ataaaagaca atctcaaatt ttattggtgc taaatatatg tgctaataaa atataggagg
    13981 gaatatttaa attttgataa agtatatgat tgcaggtgag agaatatgaa aacaggcaca
    14041 attgtaaagg tgtcaggacc gcttgttgta gcggagggca tgagagatgc gaatatgttt
    14101 gacgtcgtaa gagtttcaga taaacatctt ataggcgaga ttatagaaat gcatggagat
    14161 aaagcttcta tacaggttta tgaggaaact tccggtctag gaccagggga agaagtagtt
    14221 tctcttggaa tgcctatgag tgttgaactt ggaccaggtc ttatttcaac tatttatgat
    14281 ggtattcaac gtccacttga aaaaatgtat gatatttcag gtactaatat aactaaaggt
    14341 gttgaagtat cttcacttga tagaacaact aagtgggaat ttaaacctaa aaaatctgtt
    14401 ggtgataagg ttgtagcagg agatataatt ggtggcgttc aagaaacagc agtagtagaa
    14461 tgtaaaatta tggttcctta tggagttaag ggtactataa aagaaatata ttctggtgaa
    14521 tttactgttg aagatacagt ttgtgttatt acagatgaaa aaggtaatga tatacctgta
    14581 actatgatgc aaaaatggcc agttagaaaa gaaagaccat atagagaaaa gaaaacacca
    14641 gattcacttc ttgttacagg tcaaagagtt atagatacat ttttcccaat cacaaaaggt
    14701 ggggtagcag ctataccggg accttttgga agtggaaaaa ctgttacaca gcatcagctt
    14761 gctaaatggg cagatgcaga tatagttgta tatataggct gtggcgagcg tggaaatgaa
    14821 atgacagatg ttcttcttga atttcctgaa ttaaaagacc caagaacagg cgagtcactt
    14881 atgcaaagaa ctgtgcttat agcaaataca tcagatatgc cggttgctgc acgtgaagct
    14941 tctatataca ctggtattac aatagctgaa tattttagag atatgggata tagtgttgca
    15001 cttatggcag actctacatc aagatgggct gaggctctta gagagatgag tggtcgttta
    15061 gaggagatgc ctggtgaaga aggttatcct gcatacttag gttcacgtct tgctcaattc
    15121 tatgagagag caggaaaggt aaattgtcta ggtatggatg aaagagaagg aacacttaca
    15181 gcaattggtg cagtttctcc tcctggtggt gatacatcag aacctgtcag ccaagcaact
    15241 cttcgtatag taaaagtatt ctggggtctt gatgcatctt tagcatataa acgtcatttc
    15301 ccagcaatta actggttgac tagttattca ctctatcagg aaaaagttga tgtatgggca
    15361 gataaaaatg taaatgataa tttctcagct caaagagctc aagctatgaa acttttacaa
    15421 gttgaatctg aacttcaaga aatagttcaa ttggttgggg ttgattcact ttcagatgga
    15481 gatagattga tactagaggt tactcgttct ataagagaag acttccttca tcaaaactct
    15541 ttccatgaag ttgatactta tacatcactt cataaacaat atagaatgat gggacttata
    15601 ttaaaattct ataagtcagc acaagatgct atcaagcaaa atgttactat aaacgatatt
    15661 atcaagcttt cagttcttga aaagattggt cgtgctaagt atgtagaaga aggcgatata
    15721 gatgcagctt acgattcaat tgaagatgaa atagataacg agcttgcaga ccttattaaa
    15781 aagagggggg ctgaagaact atgataaagg aatataagtc aatacaagag gtagccggtc
    15841 ctcttatgtt aataaacggc gtagaaggtg taaaatttga tgaacttgga gaaattgaac
    15901 tttcaaatgg agaaattcgc cgttgcaaag ttctagaggt aaatggagat actgcacttg
    15961 ttcagctttt cgaaagctca acaggtataa accttgctga aagtaaagtt cgttttttag
    16021 gaaagagtct tgactttgga gtttcaccag atatattagg tcgtgttttt agtggaatgg
    16081 gtagacctat tgatggaggt ccagaaatta taccagataa aagacttgat ataaatggag
    16141 ctccaataaa tccagcagca cgtgattatc cggccgagtt tatacaaact ggagtctctg
    16201 ctatagatgg acttaataca ttggttagag ggcaaaaact tcctatattc tcagggtctg
    16261 gtttgccaca tgccaatctt gcagttcaga ttgcaagaca ggcaaaagta cgtggaactg
    16321 attctaagtt cgccgttgta tttgctgcag ttggtataac ttttgaagat gctgaatttt
    16381 ttataagcga ttttaaagca actggtgcaa tcgaccgttc tgtagtgttt gtaaaccttg
    16441 caaatgaccc agctgtagag cgtgtatcta cacctagaat ggcacttaca gcagcagaat
    16501 accttgcttt cgagcaagat atgcatgtac ttgttataat aacggatatt acaaactatg
    16561 cagaggcgtt gcgtgaagtt tcagcagcta aaaaagaagt tccaggacgt cgtggatatc
    16621 caggatatct ttatactgac cttgctacta tgtatgaaag agcaggaaaa atgaaagggc
    16681 atgaagggtc tataactatg attccaatac ttacaatgcc tgaggatgat aagacacatc
    16741 caattcctga ccttacagga tacattacag aagggcaaat tatacttagc cgtgaacttt
    16801 ataaaaaaga tatacagcct cctatagatg ttttaccatc actttctcgt ctaaaagata
    16861 aaggtattgg taaaggtaag actcgtgaag accatgcaga tacaatgaac cagctttttg
    16921 cagcttattc aagaggtaag gatgcaaaag aacttatggc tatacttggt gaatctgcac
    16981 tttctgagat tgatttaatg tatgctaagt ttgcagatga atttgagaaa gaatatgttt
    17041 cacaaggatt tagaactgat agaactattg agcagacatt agaaatcgga tggaagcttc
    17101 ttagtatgtt accaaaaagt gaacttaaac gtatccgtga tgaatatttt gaaaaatata
    17161 tgcctaagga gtgatatctg tggctagatt aaatataaat ccaacacgta tggaaatgac
    17221 tagacttaag aaactcctta aaactgctac aagaggtcat aagcttctta aagataaact
    17281 tgatgaactt atgaaacaat ttcttgagat tgtaagagaa aataagcgat tgcgtgaaga
    17341 agcagaaaat gctcttgata ctgcttataa aaactttata atcgcaagag cagttatgag
    17401 tcaggagtat ctgggttcag cacttatgat gccaaagcaa tcagtttcag tagatgtttc
    17461 tacgagaaat ataatgagtg ttgatgttcc tgtgtttgac tttaaaactg aaaacaatca
    17521 aagtgatatt tatccttatg gtcttgcttt tacatcaggt gaattagatt ctgctatgga
    17581 agctttctca gatgctatgc aaccactttt aagacttgca gaaagtgaaa aaagtgcaca
    17641 gcttcttgca caagagatag aaaagacacg tagacgtgtt aatgctcttg aaaatgtaat
    17701 gataccaaat tacattgaga ctattaagta tatagctatg aagcttgaag aaaatgagag
    17761 agcaagtact actagactta tgaaggttaa ggatatggtg cttaaaaaag cacttgaaga
    17821 aaagaaaaaa aatgatttgg ttgtataatt aaatacttat aaaaataagt ctaacattct
    17881 actttataga ggttagactt attttttatg tttaaattaa aatgtagata ctataaaaat
    17941 taatatattg tttaaaaaca gactgtattt aactaaaaat aatatattta acaaaaattt
    18001 ctaaaaatta acgaactgtt aatttacact taactttata ttaacaaatt aggtgtatta
    18061 tataaacata agcaaaaaca ataaaaaaca aataaaaaaa ggaaaaaaca caaaggggga
    18121 agatatgaaa gaatttatat ttacttgtct gtgcattgta gtgattacat ttatgacaag
    18181 ttgcatatca acaacaccac aaactactca aggaaaagga aataataaac ttacaatata
    18241 tgcagcacta gaggaagagc agatagctta ttatatggaa ggttttagag aagagtatcc
    18301 caatatagat gtagagatag ttatggattc tcatggtgta atagcttcaa aactattagc
    18361 agaaaaagat aatccacaag cagatattat atggggatta tcagcaatga acatgataga
    18421 gctaaaaaaa gatggtgcac ttgaagcata taagcctaaa aattatgata aagttgataa
    18481 aaaatttaaa gatactgaag atgatgtatc ttgggtagga atgagtgtcc cagagacagc
    18541 aatagtagtt aattataagg aacttaagaa actaggtata gatattccaa aaagttatga
    18601 agatttaata aaaccagaat ataaaggact tataacaatg ccaaatccag catcatctgg
    18661 aacggggtat cttacagtat cagggcttat tcaaatgatg ggtgaaaaag atgcttacaa
    18721 ttacatggat aaattacata aaaatatagc cacatataca cactcgggtt caaaacctgc
    18781 gaagttagct ggctctggag agtatccaat aggtattaca cttggatata gagctacaca
    18841 acaattatct agtggagaac ctatagaggt tgtatttcca aaggaaggtt caggctggga
    18901 cttagaagca aatgccttag ttaaaaaaga taatataaaa aaagaagcaa agctattttt
    18961 agattgggca ataagtgatt cagctatgga tagatatgca catgaagtag cagttacttc
    19021 cataaagaca gataatccag ttccaaaagg ttattctaaa caaccatttg aacagcttat
    19081 aaaagaagtt gatttaaact ctgctgccaa aaacagaagt acagtactta aaacttggga
    19141 atcaaaatac ggttcaaaat ctgaaagtaa gtaaagttta aattaataat ttagatgaaa
    19201 gaattttaga agaaaaagta ggatataaaa tttaaaataa aaaaataact atataaggag
    19261 agataagata tgagttactt aaaaataaat aatgtgttta aaagttatga ccaaaaaagg
    19321 gttttaaata atattagttt ggatattgaa gaaggagaat ttctatgttt actcggacct
    19381 agtggatgtg gtaaaactac attacttaga ataatagcag gtcttgaaga tgtaaatagc
    19441 ggaactataa ttctccagga taaagatatt acaaatttag agccatcaaa gagaggtttt
    19501 ggtatagtat ttcaatcata tgcattattt ccgaatatga ctgcttataa caatatagct
    19561 tttccactaa aagagagaaa agtttcaaaa ggaaaaatag ataataaagt taaggaagta
    19621 cttgaaacag ttggattgac taatgaagct cataaatatc ctaaagcatt atcaggtggg
    19681 caacaacaga ggattgcaat agcaagagca ttagctcttg aaccaaagtt tttactttta
    19741 gatgaaccaa tgtccgcatt agatgcaaag gttagacaca agcttagaat ggacattaaa
    19801 agattacaaa aagaacttaa tataacgaca ataatggtaa cacatgacca agaagaagct
    19861 ataactatgg cagataaaat agctattcta aatggaggag atataatgca aatagggact
    19921 ccagaagaaa tataccaaaa tcctcaaaac ctatttacag ctcaatttat aggagataca
    19981 aactgttttg ataatggaga ttctatatta actgtaagac cagaatatgt tcaaatagag
    20041 aaatcgacaa aagaaaacta ccaaggaata atatcaaata tagagtttag agggaattta
    20101 ttgagagttg aaataaaaga taaattaaat gaaaatttca taataagtga tgcttctata
    20161 aaagagtggg ttaatttaaa tttagtagaa ggtgaccttg taaaaataag tatagacgaa
    20221 aaatactatt taaagtatcc aaaagttaaa ggagcataag tgtatgaata ataatgctat
    20281 aagttctaaa agtataagtc ttaaatcaaa aatgaattta gatataaaaa aatgtatgct
    20341 tacagtatta gttatgtctt tagtaatatt tttattagca cctataatat atctatttgt
    20401 acaggctttt ttagataaag atggagtctt tgtaggatta caaaactttc aagaatactt
    20461 ggcttctgaa tctctattaa attctctaaa aaatacagta tttgtatcag ttatatcaac
    20521 tgtaatatca ctaacacttg cattagtata tgcatatgca ttaactagaa caaagattaa
    20581 atttaaaggt ttttttaaat cattagctat gtttcctatg tttgctccaa caatgatgca
    20641 tgcaataggt ctagtatatt tatttggaaa tcaaggtttg gtaactaatt tacttggagt
    20701 gcatgttcca atatatggag cagtgggaat aatattggca caaattatat atgtatttcc
    20761 tcaatcattt caaatacttt atatatctct aagttcaacc gattatagac tatatgaagc
    20821 tagtaatact atggggataa gcaagataag agaatttata agcataacaa taccaagtat
    20881 aaaatactct ttaataagtt ctgcattcgt atcgtttaca ttatgtttta cagattttgg
    20941 agctccaaag gtgataggag gagagtttaa tgtattatca atagatgtat ataagcaaat
    21001 tatagggcaa cagaatatgc caatgggagc aactgtatca atattacttt taatacctgc
    21061 aataatatcg tttatagtaa ctaaaaaaat tgaaaaaaat caaaattcat atataacttc
    21121 aaagtcaatt ccatataagg ttaaaactaa taagttaaga gatagtattt taggaggggt
    21181 tgtaggaatt attgcatttg gtataatagc tgtaatgtta actgtaatct tatcttcatt
    21241 aatatcagtt tggccatatg atttaagact gacactggaa catttttcta tgaaaaacag
    21301 tattgatggg attaaaccat ttataaattc aataaaaatg tcactattga cagctattct
    21361 agggacgagc tttgttttct tatttgcata tttgaatgaa aaaacagaaa aagctccata
    21421 tttaaaatca tttgggtatt ttttaagtac attgcctatg gcattgccag gattagtttt
    21481 aggtatagct tatgtattat tttttaataa attagagttt aattttatgg atacaatata
    21541 cattaagaat ttgtttaatg gattatatgg aacattactt ataatggtta tatgtaatat
    21601 agttcatttc ttttcagtag catttataac agctactact gctattaaga aagtagataa
    21661 agaattggat atggtagcta aatcaatggg aataaacagc gcatcaattc ttaagaacgt
    21721 aactatacca ctttgtttgc cagcagtttt agagaacttt atgtactttt tcctaaactc
    21781 catggtgaca atatcagcat tagtttttat atatactgca agttcaaaag tagctgcaat
    21841 atcaattgta caactagatg ataaggggag tactgcacag gcagcatcac tagctgtgtt
    21901 aatattagtc acaaatatat tagtgaaagt aatatatgaa tttataaaga atcgtttaga
    21961 aaaaagaaca aactttaatt aaataattta tcttataaaa acacaaattt aaaaaagtct
    22021 attcttgtga tttattattt aaagaataga ctttttaaat ttattattga tattgtttat
    22081 taattttcct tttgttatca tacatttgag aatctgcacg acgatatgta tcaaataaag
    22141 aaacatctac tttttcatca aaaatagcat atccaataga cattgataat tttaattcat
    22201 tagttgagtt ttcttcaact atttttaaat taatattatt tataatatta tttatttctt
    22261 tgtgagataa attttttaga ataattatgt attcatcacc tccaatacg
//
LOCUS       DAYAOY010000064        22142 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYAOY010000064 DAYAOY010000000
VERSION     DAYAOY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.064"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            863..2533
                     /gene="pdcB"
                     /locus_tag="ACGI6B_002526"
     CDS             863..2533
                     /gene="pdcB"
                     /locus_tag="ACGI6B_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HFG9751963.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2655..3389)
                     /gene="pflA"
                     /locus_tag="ACGI6B_002527"
     CDS             complement(2655..3389)
                     /gene="pflA"
                     /locus_tag="ACGI6B_002527"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFG9751964.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3549..5780)
                     /gene="pflB"
                     /locus_tag="ACGI6B_002528"
     CDS             complement(3549..5780)
                     /gene="pflB"
                     /locus_tag="ACGI6B_002528"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFG9751965.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6139..7134
                     /locus_tag="ACGI6B_002529"
     CDS             6139..7134
                     /locus_tag="ACGI6B_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFG9751966.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISILYFMYII"
     gene            7225..8838
                     /locus_tag="ACGI6B_002530"
     CDS             7225..8838
                     /locus_tag="ACGI6B_002530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9751967.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9113..11098
                     /locus_tag="ACGI6B_002531"
     CDS             9113..11098
                     /locus_tag="ACGI6B_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9751968.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11103..11537
                     /locus_tag="ACGI6B_002532"
     CDS             11103..11537
                     /locus_tag="ACGI6B_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFG9751969.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11566..12126
                     /locus_tag="ACGI6B_002533"
     CDS             11566..12126
                     /locus_tag="ACGI6B_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFG9751970.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12190..12690
                     /locus_tag="ACGI6B_002534"
                     /pseudo
     CDS             12190..12690
                     /locus_tag="ACGI6B_002534"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            12730..13149
                     /locus_tag="ACGI6B_002535"
     CDS             12730..13149
                     /locus_tag="ACGI6B_002535"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HFG9751971.1"
                     /translation="MAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFP
                     LLSPFLGPGAVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAE
                     AEPDTVQVGVPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13190..13555
                     /locus_tag="ACGI6B_002536"
     CDS             13190..13555
                     /locus_tag="ACGI6B_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFG9751972.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13656..14429
                     /gene="srlD"
                     /locus_tag="ACGI6B_002537"
     CDS             13656..14429
                     /gene="srlD"
                     /locus_tag="ACGI6B_002537"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFG9751973.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14778..16922
                     /locus_tag="ACGI6B_002538"
     CDS             14778..16922
                     /locus_tag="ACGI6B_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFG9751974.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            17010..17345
                     /gene="spoIIAA"
                     /locus_tag="ACGI6B_002539"
     CDS             17010..17345
                     /gene="spoIIAA"
                     /locus_tag="ACGI6B_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HFG9751975.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17359..17787
                     /gene="spoIIAB"
                     /locus_tag="ACGI6B_002540"
     CDS             17359..17787
                     /gene="spoIIAB"
                     /locus_tag="ACGI6B_002540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HFG9751976.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17810..18574
                     /gene="sigF"
                     /locus_tag="ACGI6B_002541"
     CDS             17810..18574
                     /gene="sigF"
                     /locus_tag="ACGI6B_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HFG9751977.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18854..19291
                     /gene="spoVAC"
                     /locus_tag="ACGI6B_002542"
     CDS             18854..19291
                     /gene="spoVAC"
                     /locus_tag="ACGI6B_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HFG9751978.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19458..20471
                     /gene="spoVAD"
                     /locus_tag="ACGI6B_002543"
     CDS             19458..20471
                     /gene="spoVAD"
                     /locus_tag="ACGI6B_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HFG9751979.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20485..20847
                     /gene="spoVAE"
                     /locus_tag="ACGI6B_002544"
     CDS             20485..20847
                     /gene="spoVAE"
                     /locus_tag="ACGI6B_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HFG9751980.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21030..22046
                     /locus_tag="ACGI6B_002545"
     CDS             21030..22046
                     /locus_tag="ACGI6B_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9751981.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8639 a         2487 c         3840 g         7176 t
ORIGIN      
        1 aagaaccctt ttataacaaa tggaaactat ggatgtaata attaaaataa aaaatattat
       61 tttatattta taaacattga cattctaatt ttgtagtgta atcgtatttt ttattaattc
      121 ttacaaaatc aatgaaaata agtatatcca ttttaaagta cataaattta aaaatccaga
      181 aaaatatact taaattatgt aagtaaaaga ggggttgaat cagttgaaaa tgtaatatta
      241 gtaaatagta catcaatcct atataccctg taagcaactc tattttcatg tactaaaaat
      301 gtacctattt tattaactga aaagaataaa ttaaatgtaa gtacataaga agatagaaaa
      361 aattaaaaac aaaaaatatt tatataaata aatataaaaa atgatataat ccctattaaa
      421 aaatttatag atgatgaaga aatatcaaaa tcttatatta taggaagaga cacttctttg
      481 ccaaaatcta tagaaaagca ataccaaatc caaagagaat aggcgaagaa aaaaataaaa
      541 taaattcaag agtaatagaa aagttttata gaataaagaa ctgataactc tttatataac
      601 gaaaaataga attttaagag aaaatgattt aataaatgat ttaacatggt gttttagcag
      661 gtaaaaataa atcaccagta atgattatag aaaaattact ttatgaatct caaaaaactt
      721 agttaagagt aagagatttg ataagataac taaagttggg gaaatgtgtt tgatgagaca
      781 aaaaattagt aaaatagtat aatctaaata aggatatttt gttgattaaa ctacactaat
      841 agtaatattg tagggggaaa agatgagaga agaatcgaac tctttaagtt tagaccatat
      901 agaaattttc tttgaacttt tatctcaaag tacagaagat tatatatttt tttgggatat
      961 aaataggaat aaatttaaaa tttcttcagc aatttttgat gaatttaact taagtaaaga
     1021 gatagaatct gatttggtca attgttggag taaaattgtt tatccggatg atgttcaaat
     1081 atggaaagat gatatacaag aattactaca tggaaaaaaa ggtgagcaca atttagaata
     1141 taggttgatt aataaatata aagaaatagt atggatttct tgtagaggta aagtttatgt
     1201 gtcggatgac ccaaaaacta tatttctagt cgggagaata aagaatattg gtgaaaaaaa
     1261 taaatttgat tctatcacag gaacgtggaa tagagaacaa tttgaacata gaatgaatta
     1321 tcttataaaa gagaaaattt ataaaaatgg tgctatgttt attatggata ttgataattt
     1381 taaaaatata aatgaaaaat atggacattc ttatggtgac aaagtattgc gtgcaattgc
     1441 tactgaagta ttagaatatc ttccaaaaga tgtaagattg tataggttag atggagatga
     1501 atttgcattt ttttacccta tgtgtactaa agaaactata gagaaaatat atgaaaagat
     1561 acagatgtat acaaatactc agcatgaaat tgaatcaaat aagtactatt gcacagtgac
     1621 tgcaggcgtt gctatgtatc cagaagatgg agataattat ttagatttat ttaaacatgc
     1681 tgatattgca ttggatatag caaaaattag tggtaaaaat agaattaagt ttttttcaca
     1741 agagctctat gaaaataaac taaaggtaat ttcaatgcaa caaaaactaa gagagtgcgt
     1801 tgaaaataat tttaatgatt ttgagttgtt ttttcaaccc caggtaaatg ctgttacaaa
     1861 agaggtaata ggggcagagg tacttttaag atggcattcg agtacttatg gagaagtatc
     1921 tcctgttgaa tttataccaa tactagaaca gagtaattta attataccag ttgggaagtg
     1981 gattataaaa gaagctgtaa aacagtgtaa agaatggcat aaaataaatc cagactttaa
     2041 aatatctgta aatgtatcgt atattcagtt aaaggaagat ttttttagag attttatagt
     2101 agaatgttta gtagagtatc aattaagacc tgaattttta attttggaat taacagagaa
     2161 ttgttggata ccagatataa atttactaaa tgataaattt attagtttaa agggtatagg
     2221 agtatatatt gcaatagatg actttggtac aggatattca tctttaaatt accttaaaga
     2281 actgtctgtt aatataataa aaattgaaag aagttttgtt aagaatatta catataatag
     2341 ttatgaatat acttttcttg aatatattat taaattagct catattataa atttaaaagt
     2401 ttgtgttgag ggaatagagt cttatgaaga gtatgatata gtaaaaagct taggtgtaga
     2461 tattattcag ggttttttat ttggaagacc tgtaagtgcc tctgaatttt ataaatttca
     2521 gctatcaaag tgatgtatat aaaatgagat atatagtata taaaaatagg gcaaagtgga
     2581 agttttgaag tttattaaaa atttacactt taccctattt tgttatgtct tctataaagg
     2641 attaatatac tacactaact attatatact ttcactccaa atttagagat tatttcacta
     2701 gctttttgga catcttcctt agtggcatca ggaacatctt ttagttcata atcgatacct
     2761 aaactttccc atttgtgtat tcccatagaa tggtatggta gcaattcaaa tctttctaca
     2821 tttttcaatg tagctacaaa ttgacctagt ttttctaaat tatctatatc atcagtaatg
     2881 ccaggaacta aaacatgtct tatccataca ggaatatttc ttgattctaa gtgtctagca
     2941 agttttaatg ccttatccat accaacacca gtcaaatcaa tagatttttc ctcaaccata
     3001 tgttttaaat caagtaaaac caaatcaata ttatctaaaa ctgggtctat agtttctata
     3061 tctacaaatc cagatgtatc caagcaagtg tgtatatcat tttcttttgc ttttttaaat
     3121 aattcactta aaaattctgg ttgaagagtg gattcaccac cagaggcagt tatacctcca
     3181 ccagaaaact tcataaaaga agtatactta agagcatccg ttattatttc atcaactgtg
     3241 tattcttttc ctgatttaat atcccatgta tccctgttat gacagtattt gcatctaaga
     3301 ggacacccct gaaaaaataa tatatatctt attcctggtc catctacagt accaaacgtt
     3361 tctatagaat gtatctttcc cttaatcata gagttcacct cacaatatat tttaataacc
     3421 ttatcaatag ttataatatt tctctaaaaa aggccatctt ataccagaaa taattcaaat
     3481 ataagatagc cttttatttt tattttttac acagtagtaa tcttagttaa agtataaaaa
     3541 tttaaagact aagccatttt accgtggaat gttctgttta taacatctaa ttgttgctct
     3601 ttagttaatt ttatgaagtt aactgcatat ccagatactc ttatagttaa ttgtggatat
     3661 tcttctggat gttccatagc atcttctaaa gtagctctat caaatacatt tacatttaag
     3721 tgatgagcat tttgagcgaa gtatccatcc atcattgcag aaagattgtt tattctttca
     3781 gtcatatctt ttcctaaagc accaggtact atagagaaag tattagatat accatcttga
     3841 gcatgttcat atggtaattt agcaactgaa gataatgaag ctaaagcacc attagtatct
     3901 ctaccatgca ttgggttagc accaggagca aatggagcac cagctcttct accatctggt
     3961 gtattaccag ttttcttacc ataaacaaca tttgaagtta ttgtaagtat agattgagtt
     4021 ggataagaat ttctataagt tttattcttt cttattttat tcataaagct ttcaactaac
     4081 tctacagcta tactatcaac tctatcatca ttatttccgt attttggata atctccttct
     4141 atttcaaaat caacagctat tccttgttca tttctaatag ttttaacctt agcatgtttt
     4201 atagcagata atgagtctgc acaaactgat aacccagcca taccacaagc catagttcta
     4261 aatatatctc tatcatgtaa tgccatttct aatgcttcat aagaatattt atcatgcata
     4321 taatgtatta catttaaagt atttacatat aatgttgcta accaatcagt gaatggttca
     4381 aatttactca taacttcatc ataatctaag tactcagaag ttataggttc aaatcttggt
     4441 ccaacttgaa caccagattt ctcatctaca ccaccattta tagcgtataa taaagtttta
     4501 gctaagttaa ctctagctcc aaagaactgc atttgtttac caattctcat tgcagataca
     4561 caacaagcta taccatagtc atctccccag tatgctctca ttaaatcatc attttcatac
     4621 tgtactgaac ttgtatctat agatacttta gaacagaaat ctttgaatcc ttgaggtaat
     4681 ttagttgacc atagaactgt taagtttggt tctggagaag gtcctaatgt atatagagta
     4741 tttaacattc taaatgagtt tttagtaact aatgttctac catctattcc catacctcct
     4801 atacactcag ttacccaagt tgggtctcca gagaataggt tattgtaatc aggagttctt
     4861 aggaacttaa ccattcttaa tttcataaca aaatggtcca tatactcttg taattcttct
     4921 tctgttacga ttccagcttt taaatctctt tcaaagtata tatctaagaa agtagatgtt
     4981 ctacctaaag acattgcagc accattttgg tctttaacag cacctaaata accaaagtat
     5041 aaccattgta cagcttcttt tgagttagtt gctggttttg atatatcaaa tccataagat
     5101 tcagccattt ttttaagttc atttaaagct gatatttgct cagttatttc ttctcttaat
     5161 ctgataactt cctcatccat gcaagaaact tctaatgatt tcttttgctc atttttatct
     5221 tctattaaag catcaacacc gtatagagca actcttctgt agtcaccaat tattctacct
     5281 ctaccataag catctggaag acctgttatt ataccagatt ttctagcagc tctcatttct
     5341 ggagtataaa catcaaaaac tccttggttg tgtgtttttc tgtatttagt aaatatatct
     5401 tttatttctg ggtctaattc atatccaaaa gcttcacatg aattttcaac catctttata
     5461 ccaccatatg gcatgacagc tctttttaaa ggggcatccg tttgtaaccc aactattgtt
     5521 tctaattctt tatctaaata tcctggagca tatgcagcta ttccagaaac agtttttgta
     5581 tcaacatcta aagtaccacc attttctctt tcttttttaa ataggaccat tgcttcgtcc
     5641 cataattttt tagtattttc agtggcacca gctaagaaag agtcatttcc ctcatatgga
     5701 gaatagttta attgtataaa ctctctaaca tttatttctt tagtccatct tcctgtttta
     5761 aatccttgcc atgcattcat taagtatttc ctccttagat ttaaatgata aataatacta
     5821 ttaaaattaa tttttatata cttaatttat tttatatatt tttttaaaaa tatattttct
     5881 aatataataa tagcatatta ttcgtatcct gtatacataa aaaataaaaa attgtcgctt
     5941 taaagtataa aacagattat atgttaataa tgtggtgaaa ttatacccat atataaataa
     6001 aataaaacaa ataaatagct aattaaggag aaaatatttt ttattttttg tttaggataa
     6061 ataaatttta ttatggaaaa aagaaaaagc atacatcttc acaaactgaa tatttattta
     6121 atagggggag gtgatagatt gtttatcaca gtggtattat ttttagtagg tttcctatta
     6181 attactaaag gcgcagatat ctttataaat tgtactgtag agataggtaa aaaaacgaat
     6241 atatcagaaa ttatacttgg agctactata gttagttttg caacaacatt acctgagttt
     6301 acagtgtctc tacttgcttc tattgatggt catactacta tgagtttagg aaatgcagtt
     6361 ggttctataa tatgtaatac agggttagca ttagggttgg ttgtttttat aagtccattt
     6421 aatgtagata aaaaaatgtt tttttctaag tctttactat taatagtatc cgttatagtt
     6481 ttaattttat tgagtttaga tggagttata acaagaggtg attcattatt acttataata
     6541 atattaatct tttatatggt taataattat agaagtgtcg ttggaaaatc tgatactaaa
     6601 aatagaaata ttaaaaataa cactattaaa gacatatcta ctaaaaaagg gaaaaattat
     6661 agaggttttt ccgtattaga aattgtaaaa atactattac tttttgctac aggattgata
     6721 atgatgataa taggttcaca aatccttata gagagtggag taataatagc tagtttttta
     6781 aatatacctc aaggtatagt aagtttaact ataatagcac ttggaacttc tctgccagag
     6841 atagtttcgt cgattactgc tataagaaaa aatcatcatg aaatatcagt aggaaatatt
     6901 ttgggtgcaa acatattgaa tatagtatca gtaatagctg tatcagcaat accaaataac
     6961 ataccaatac tatctcaaaa tagacaattg gatataccat tcatgatatt attattatta
     7021 atagtaataa taccaacact taaatctaat aaacttagta gaatacaagg tatattgatg
     7081 ttattcacat attttctgta catatcaatt ttgtatttca tgtatattat ttaactatac
     7141 tgtttaaaat attaattgca gaatatccta aaaaagtata taatggatag gttaagatta
     7201 attgataacg gagaggaata atatatgaat ataacaaagt ttgaagattt accaataagt
     7261 gagggaataa aaaaagctat agctgaaatg gggtttgaag aaccatctcc tatacaagca
     7321 cagtctatac ctgcgatttt gtcaggtaaa gacgttatag gacaagctca aactggtacg
     7381 ggaaaaacag ctgcttttag tatccctata ctagaaacaa tagacccaaa taatagaagt
     7441 ttacaagctg tagtgctttg tccaacaaga gaactggcaa tacaagtatc aacagaaata
     7501 agaaagttag ctaagtattc gcacggaata aagacattac ctatatatgg tggtcagcca
     7561 atagatagac aaataaaatc tctaaagagt ggtgtgcaag ttgtaatagg tacaccagga
     7621 cgtacaatag accatatcaa tcgtaaaaca ttaaagatgg ataatgtaaa aatgattata
     7681 ttagatgaag cagatgaaat gctagatatg ggatttagag aagatataga aatgatttta
     7741 agtaagattc cagaagaaag acaaacaact ttcttttcag ctactatgcc aagaggaatt
     7801 ttggaattaa ctaagagata tcaaaaagac ccagagcata taaaagttgt tagaaaagaa
     7861 cttacagttt caaatacaaa gcaatactat atagaaacta ggtcatcaaa taaattagaa
     7921 gtattatgta gattagtaga tgtatatgac cctaaactat ctgttgtttt ctgtaatact
     7981 aagagaaaag cagacgaatt ggtaggagat ttacaagcaa gaggatattt tgcagatgca
     8041 ttacatggtg acttaaaaca gactcaaaga gatatagtaa tggataagtt tagaaatggt
     8101 acaatagata tacttgttgc tactgatgtt gcagctagag gtatagatgt tgatgatgtt
     8161 gaatgtgtat tcaactatga cttaccacaa gatgaagaat attacgttca tagaattggt
     8221 agaactggtc gtgctggtag agaaggtatg tcatttactt ttgtatttgg aaaagaaatg
     8281 agaaagatga aagatataga aagatatact aaatctaaac taataaagca taatatacca
     8341 acaataactg atgttgaaga gaaaaaagta ggtactttct ttgcacaagt taaacaaact
     8401 atagaagaag gtcacttaac taaacaatta caatggttag aaggtttctg taatgatgaa
     8461 gattatgcaa tggttgatat agctgctgca cttgtaaaac tatctctagg tgaagaaatg
     8521 aaagaagaaa taatagaaga aaaacctaga agagagcgtg gagatagaaa aggtggtact
     8581 ggagctaaag atggtatgat tagattattt atcaatattg gtagaaatca aagagtacaa
     8641 gcaaaagata tagttggtgc catagctgga gaagttggaa taccaggtaa agtagtaggt
     8701 actatagata tatatgataa gtatactttt gttgaaatac ctaaaaaaga tgcaaaaaca
     8761 gtaattgaaa agatgaaaga tattaagata aaaggtaata agattaatat agaaaaagca
     8821 aataagaaga aaaaataacg tatagtatac aaaactataa ttagaaaata tataaaataa
     8881 tatattggat atttaaaaag cctgatgtgt tttcaaaaca gatagtagat aatctgtaag
     8941 agttaacagc ataaggcttt tcttgtttta tatgatgtta taattttcca gattgttgtg
     9001 aaaaatgtat attagttcct ttttttcaaa tctgatttga aaaagagaat attaattatc
     9061 tatatagttt agattataat aaaaaatgta agataacaaa ggggagaaaa agatgtcaat
     9121 gacttatatg gaccctaggg tttttttgat tttagaaatg gtgttagaag aagacacaaa
     9181 tactctagaa caattttcaa atgttttggg tgttagtact aggactatca gaaactatat
     9241 aaaccagatt aatcgagaat taaaaggtgg tattgctaaa atagttaaaa atgaaaaagg
     9301 tacatatagt ataagaattg aggatgaaag taaattaatt gagattgtaa attttaatag
     9361 aggaaagcta tcaaatttta taaatttaaa ttcacctgat gaaagaataa attatgttct
     9421 tgatatttta actatgacca acaatccaat taccatagat gatttggctg aagagatatg
     9481 tataggaaga acaacattaa taaaggattt aaaaaaagca gacagaattt tagctgaata
     9541 tgatttagaa ttaaaaagga agccaaatat aggtatgatt ttagttggtg aagaaatgga
     9601 tataagatta ttaatccttg atagattgta tgaaaattat attgatgtct tggaaaacgt
     9661 taatcagata aatttagtta aagattatga tattgaatgt cttagagatg aattaaaaaa
     9721 gttatttaaa aaagaagaac tctacataac agaacaggtc ttgagagatg ttgaaagata
     9781 tataatcatg tctgtcttaa gaaatctaaa tgggtacaaa tttgaaaaga tagataagag
     9841 atttgaagtg ataagatgtt cagatgaata tactttagga ttaaagctca aagctttatt
     9901 ggaaaagata tttaatatag ttttaaatga aaaagaaacc atatttttga caatgccatt
     9961 aataggtaga aaagctccta gtacaaaatt agcacttagc agtataaaga taaatgatag
    10021 cgtaaaggaa gttgtagatg aagtaactag ttttttatta gagacctcag ggattgattt
    10081 taaagaagat aaaaagctta tagaaaattt ggaatatcac ttgtattttg cacttaatag
    10141 aatgagattc aacataaggg taaaaaatcc tcttttacaa gaaataaagg aaagatatac
    10201 atttccatat agtgttgcaa agatagctgc aatgataatt gaggaaaagt ttgacttgaa
    10261 aatatgtgac cacgaaatag gatatatagc attacatttt ggaagttatt ttgaaaaaaa
    10321 ttcaagaaaa gttctaaatg taaatagggt agctgttgtt tgtggaacag gtcttgggac
    10381 tgcacaactt attagaataa agataggaaa aatacttgga gaaagtgtag aagtaaatac
    10441 tttttctgat atagaagcta aagctaattt agatttatta aagtattatg atttagtatt
    10501 tacaacagta gatttcaaaa cagacttaga tgttccaatt tttagagtaa atgcgttatt
    10561 taatgaagaa agtataaaga aagagataga aaatgcaata cttttgaaaa atagtataaa
    10621 tcttaagtct ataaaaaaga ttgaaaaaaa tatccctttt ataggtaaat taattgaaga
    10681 ggataagttc tttatattga ataagagtac gtttatggaa aatttagaag atatgttaga
    10741 aaaattagtt cataaaggaa ttattgatat agagttcaaa aaaaaggtat taaaaagaga
    10801 aaagaaatct tcaacagcat tagataatta tatagcattg cctcactcag ttaatactaa
    10861 tggggaaaat ctttatctgg catttggaat attagaaaaa ccagtgattt gggatacaaa
    10921 ggaaattaga ataataatat tgatgataat tccagataag aatgtagata gtacagacct
    10981 gataataaaa agttatgaag aagtacttga agtagggaga gataaaaaga tggtagaaga
    11041 acttgctaag gcaaaatcat ttgatgaatt ttataaaata attaccaaga ggaggtaata
    11101 atatggaccc agtaattttt attatagcat cacttattac agtgtattta ttaaatattt
    11161 ttcttggata cttacagctt aaagatttta ataaaaatta tattgaacta aaaagaaaag
    11221 gaagagtggc aattggcaga aaaaaaggaa gaatttcatc gggtacaatt gtgttgatac
    11281 ttataaatga tgatggtgtg attgtcgaga ctagaaaaat gcaaggtgtt acagtattgg
    11341 caagagtaaa gtcatttgaa ggtttggtag gaaagaactt agagtatata gaaaaatcag
    11401 atttaactgt ttacaataaa ctacttaaaa aagcaatact agatgcagtc aaacagtaca
    11461 taacatttga gaaaaataaa tgtgaagata caaataatct gagtactgat gaagagtgta
    11521 ggatggaagt agtttaaaaa aatatattta taaggaggag ttaatatgac gcaatttttt
    11581 caagtattag caagtggtgc agatagtttt atgaatttat ttagggcagg tgggcaacaa
    11641 tttgtaggtt ttgtaacaga tatagtacca cttttagtag cattattagt tacaatgaat
    11701 gcaattatca actttattgg tacagataga gtcgagagac ttgcaaaaaa atgttctgca
    11761 aatatattta ctagatattt gatattacca gtccttggta catttgtatt tgcaaaccca
    11821 atgacactat cattaggaag attcttgcca gaaaagttta aacctagtta ctttgcagca
    11881 gcaagctttt catgccatac aatgaatgga ttattcccac atatcaatcc aggtgaacta
    11941 tttatatatc taggaattgc aaatggtata acaactttgg gattctcaac agcagattta
    12001 gcagttagat atttgttagt gggtatagtt gcaaacttta ttaaaggtgt agttactgac
    12061 tatacaacaa aatttgtaga gaaacaacaa ggaatagcat taaattcaga tataaagatt
    12121 ggataattac tttaaggtat aaataaattt ttcaaataaa aagatagtgt atagaggagg
    12181 ataaattata tggcatttaa caaagttaaa atagtaaaag gttctggagg atggggtgga
    12241 ccattgataa tagaaccaac agaaaagaag aataaggtag tatatgtgac tgggggagca
    12301 aagccagaaa ctgcagtaaa aatagctgaa cttacaggat gtgagttaat agatggattt
    12361 tcacagggtg ttaaagatga tgaaatcgca tgtgtaataa taaattgtgg tggaacattg
    12421 agatgtggta tatatcctca aaagaaaata ccgactataa atataatgaa aacaggaaaa
    12481 agtggaccat tagcaatgtt tataaaagaa gatatatatg tttcaggggt aaaaccaaca
    12541 aatgtagagc agttataatt gaggtagtag gagggaagaa atatggctta tgatactaaa
    12601 aagaagataa gtgagcagag tcaaggaatt gttgcaaaaa taggtatagc actaggaaat
    12661 gtgacaagta ctctttttca agctggtagg gatactatag atacattaat acatactata
    12721 ctgcctttta tggctttcgt tgcagtccta ataggtataa taaatcaatc aggtatagga
    12781 aattgggtag ccaatttact tactccatta atcggaaatg ttggaggttt agttgtctta
    12841 tcaataatat gttcatttcc gttgttgtct ccgtttcttg gaccaggagc tgttatagct
    12901 cagatagtgg gagtattggt tggtacagaa ataggaaaag gaaatattcc accaagttta
    12961 gcattaccag cattgtttgc aataaactca caagcagctt gtgactttat accagtaggt
    13021 cttggtcttg cagaagctga acctgataca gttcaagtag gagttccgtc agttttatat
    13081 tctagatttt taacaggtgc accaactgtt ctattagcat ggttagcaag ctttgggtta
    13141 tataaataga agaatacttt ataaataatt agagagaggt tcagttgata tggaattaat
    13201 atacagtact caaataaata aaataggaga taatgcaaat gaatttttac aacataatat
    13261 gtttgtaact ttcaaagata atgctcctca agagttagaa aattactgtt atatacattc
    13321 tgaaaataat ttagtaaaag aaatacttac ggatgatgtg ctatcaataa ataatgttga
    13381 ttacaaaatt acttctgttg gtgagttagt aaaccaaaat ctttcagaat taggtcatat
    13441 aacatttaag tttacaggtg aaaaagatgg agcaattgga ggaaccttat atcttgaaga
    13501 aaaagaaata gcacctattg gcgtaggtac tatactaaaa ataattagaa aataaaattt
    13561 aaaacgaatc taatcatatt ataagtaaat tatagataaa agtcaattta actaatcaag
    13621 ttaactttta atgtgataaa aaaggggaaa tgaaaatgag aaaagtggct ttagtcgttg
    13681 gaggaggaag aagtttaggt gaatatttat ctaaacattt agctaaagta ggttatgatg
    13741 ttgctattgc agatttaaat gaagacaacg caaaaaaagt agctgaagaa ataaaaactg
    13801 aatttggttg taaatctgta ggatatagtt gtaatgccat agatgaaaga caggttataa
    13861 gtactgtcaa agctattgaa aatgattttg atagaataga tttattagtg tacaatgcag
    13921 gagtggctgt aagtaaaaaa ataactgatt ttggatacaa agattttaaa tttattgtag
    13981 acgtaaattt aaatgggtac tttctatttg caagagaagt atctaaagtc atgatagcaa
    14041 aaggtattga gggtagaata atacagataa attcaaaatc aggtagagtg ggaagcaagc
    14101 ataattcagg atattcagca ggtaaatttg ctggagtagg acttactcaa agtttagcgc
    14161 tagatttggc agaacataag ataactgtaa atgcactgat gctcggtaat ttacttgatt
    14221 ctgatatgtt tgaaagttta attcctcaat atgcagtaaa attgggaata cctgaatcag
    14281 aagtcaagca agtttatata gataaagtac ctttaaaaag agggtgtaga tttatagatg
    14341 ttgcaaatat gcttacattt tatgcttcaa aggaagctga atactgcact ggtcaatcta
    14401 taaatattac tggtggacaa gttatgtaag gtttttatgg agtgtctcaa gatgaactct
    14461 ttagttcatg aggcactttt ttgtataaaa ttaaatatat tttcagcatt taaacaatga
    14521 aaaagaggtt tatctttatt ttgagatagc ctcatttttg taattaaaaa acatagtgtt
    14581 taagtaaaca tataagattg ttagtttttt aaaagagaat tttttaataa tattatattt
    14641 atgaagtatt tttaaactta ttttgataaa atatagctat gttttatata ctgtagtttt
    14701 gaacatagaa agtttaataa attatagtat atatgttaat ttttaataag gtaagaaagt
    14761 agaaagaagg atattttatg gatataataa aaaaattaag tcatgggtgt acattagatt
    14821 gtcatgactg ttgtaaattc aatgtttata ctaaaggaaa taatgtagta aaaattgaag
    14881 gagataaaaa tcatccttat acaaaaggtt ttatatgcaa aaaagggatg gcccatttag
    14941 atagattaaa tcataaagat agaataaaga ctcctatgct taaagttgat ggagtatgga
    15001 aagagatttc ttttgataaa gctatagaaa taatggcaga aaagcttaca tactataaag
    15061 aaaagtatac atcaaagtct gtcatgcatt atgaccaata tggaagtggg tctgtgctaa
    15121 aatacatagg agatatattt tttaattttt atggaggtgt tagcagacat aaaggtggtc
    15181 cctgttggag tgcagggatg catgctcaaa aatatgattt tggagtagct aagagtcatg
    15241 ctatagaaga tatgttaaat agtaaaagta tatttgtgtg gggaaaaaat ccagcatata
    15301 ctacaattca tactatgcaa ataattaaaa aagctaaaga aaaagggata aagatagtag
    15361 tgattgaccc catatataca aaaacagctc aaatagcaga taagtatgta caagtaaatc
    15421 ctggaacaga tggagcttta gctatagcta tggcaaaaat tatagtagaa gataaattat
    15481 atgatgaaga atatataaac tcgtatgtta ttggttttga agaatataaa aaatatttat
    15541 cttcattgga attaagtttt ttaattgatg aatgtggtgt taaagagaat gatataagag
    15601 agttagtgga tttatatact aacaaatatt caagtataaa tgttggatat gggcttcaaa
    15661 aatataaaaa tggtggaaac acaataagag caatagatgc tttaggtgct ataactggtc
    15721 aaataggatt tagtggtgga ggggtaaact atgctaataa agtttatccg agtgttataa
    15781 attctgaccc ttacaatagc caatcatatg gtgaagatag ggagttttat gtaagtaata
    15841 tcagtaaatt tatagaagaa tctttaaaaa atacatcaaa taaagtaaat tatgcatcag
    15901 atgaacttga tatgacatca aataaagtaa attatgtatc agatgaactt gatataacat
    15961 caaataaaac agattatata tcaaatgaat tgtataattt atctaataag tcaatcaaag
    16021 gtaatattcc tataaagatg gctgtaataa ctaaaagtaa tatgctgaat caactgccag
    16081 atttagtaga attggagagg gtgttttcaa aaattgaatt taaagtatgt tttgatatgt
    16141 ttataactga tacagctact ttatgtgaca tgtttatacc gtgtacaaat acactagaga
    16201 gtgaagatat aatttatagc tcaatgacca atccatatat aacctataat gaaagagctg
    16261 taaagccagc acataaattg atggatgaat attacttctt tatggagcta gctaaaaaga
    16321 tgggattaaa tgattatcct tttgtagaaa agagaactta tttagaaaaa gtgattgaac
    16381 cacttaagcg ttttgacaaa aatctggata tagaaaaact taaaaacaat tactttacta
    16441 tacataaccc tgttgcttgg gaagataaga agtttgagac tccatctgga aaatacgagc
    16501 tttattcaga aagtataaaa aacttgggaa taagtccaac acctgtatat ataagcaata
    16561 aatataaaga aatagaagat aaaaatattt cttttagatt attaactaat catcatgcag
    16621 atactttatt tagtcagcat tttatggaca aaaaatctat agctcaagca tatataaatc
    16681 ggagaatggc taaaaaagta ggtattgagg acaaagatat tgtgatattg aggtcaaaaa
    16741 aagcaaaaat aaatgtacaa ataaatatag atgatggagt aggtaattat atagtaaaaa
    16801 tgtatgttgg ttggtggaag aaacatggaa atcctaatag tttaacagac actggtatat
    16861 cagattttgg gggtcaggta acatataatg aaagtatggt agaaataata cgacaaaact
    16921 agttaaattt cattgaagga ataaaaatat aattatagaa ttgattaaaa gttactattt
    16981 ttacaaaact attgaaggag ggatttttga tggtaaatta ttccttagaa cataagaatc
    17041 tatacataga atttatgtgc tcagaactag accaccatgt agccaatgaa ataagggaag
    17101 aaatagataa cttattaagt gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata
    17161 ttaattttat ggattcttca ggtataggcg tcatcatagg tagatataaa aagatttcta
    17221 atgaaggggg acgagtttca gtaattaata taagttcacg agttaagaag atttttgatt
    17281 tatctgggtt aaataaaatt attggcattt atgatacgta tgaagaagct ctaagttctt
    17341 tgtagggagg agtatgtgat gaataatatt atggaagtta aattttcggc aatatctgaa
    17401 aatgaaagtt tagcaagagt tatagtagca tcctttgcag ctaagttaga cccaacagta
    17461 gatgagttag tagatataaa aactgctgtc tctgaggctg ttacgaatgc tataatacat
    17521 ggatatgaag aagacagttc taagtttgtt ttcttaagat gtgagataga aggaaatact
    17581 ataaagatta tagttgaaga tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa
    17641 ccactttata catcaaaacc agaacttgat agatcaggaa tgggattcac agtaatgaaa
    17701 agctttatgg acgatgtaga ggtaagttct gtaaaagata atgggactag aattgaaatg
    17761 actaaaaaaa taaatgtgcc taaataaagt ccaaaagtga ggttttatta tggaagtaac
    17821 tgttgccaga gaagaaaaaa aacctcttct tagtcatgaa gagactctag aacttattga
    17881 aaaagttcaa aatggagatg aagaggcaaa agaaatatta atatcaagca atttaggtct
    17941 agttaggagc gttgtatcta gatttttaaa tataggatat gatagagaag atttatttca
    18001 gttaggttct ataggcttaa taaaatcaat atataaattt gacccaaaat ttaatgtaaa
    18061 attctctact tatgcagtac caatgatttt aggtgaaata aagagatact taagagatga
    18121 tggtatgata aaagtatcaa ggtcgttaaa gcaaatagct ataaaggcaa agatggaaag
    18181 tgaagcttta actaaaaaat taggtagaga gccgagtata gaagaattag ctaaagcaat
    18241 agatgtagaa aaagaagatt tagtaatggc tatggaagct aatttcaatg ttgagtattt
    18301 gcaaggagtt attcatgaag aggaaggttc tccaatatgc cttattgata aaataagcat
    18361 gaaaggagaa agtgaagaag aaaaagtagt ggataacata ttgttaaaag acatacttgg
    18421 aagactggat aagagagagc gtcaaataat agttctcaga tactttgaag acatgactca
    18481 aagtgaaata ggagagatgc tcaatatatc acaagttcaa gtatctagga tagagaagaa
    18541 agtattgtct aagttaaaag aatatatatc gtgataatac ttatgatatg tagaataata
    18601 aaatataata aatatattac taaaataaaa tgtaataact ttttatgtta agtttttaaa
    18661 ttattaaaag atattaaaat aataaaaagc attaaaaaag taaaaatata aaaacaaggt
    18721 gtctttatgg aaaaatagat tttttcttta ttaggcatct tttttatttt ttgaccaaac
    18781 tttagatagt caaaataaat aataggttta tttttttaaa attaacaaat aatactttag
    18841 agaggtgttt agtatggata aaaattataa aaaatatgta gaccaaataa gcccaaaacc
    18901 tacatattta aaaaactata cacttgcatt tattgtaggt ggaattattt gtatgatagg
    18961 gcaagcaata aatgatttgt atatgaaagt tggaggtctt gataaactag gtgctagttc
    19021 tgcaacatca ataacattaa tatttatagg ggcattttta actggattag gagtttacga
    19081 cctaatagga aaaagagctg gagctggttc tataattcca ataacaggtt ttgctaactc
    19141 aatagtatct ccagcaatgg agtataaaag agaaggctat gtattaggtg taggtgcaaa
    19201 cttatttaaa attgcaggac cagttttagt ttatgggata ggttcttcta ttttatgtgg
    19261 aattatttat tatattttta agatgtttta gatgattttt atttaattta aattttatta
    19321 attatgaaga aggtataaaa tctgaaaata gctactaaag taactaattg aaaaataatt
    19381 ctgcagattt aattaatatt tttaattaat attaaaaatt tgaatagtaa aatgcaaaat
    19441 taaaaaggtg tgaaaatatg aaaaataaaa gaattggaaa aagaacagtc aagttagaaa
    19501 ataaaccaac cataatatca actggaacta tagtaggtcc taaggaaggt gaaggaccac
    19561 taaaggacta ttttgatatg attatgactg acgatttata tggtgaaaag acttgggaat
    19621 tagctgaaag taaaatggta gagactgcat ctcaacaagc tatccaaaag gcaggtaaaa
    19681 aattgtcaga tgtaaattat atgctaggtg gcgatttaat aaatcaaata gttccagcat
    19741 cctttgcagc aagagaactg gctattccat ttttaggtat atatggagct tgttctacaa
    19801 tggcagaagg tctttgtata ggttctatgc ttatagatgg tggttttgct gatttagtac
    19861 tgtcaggtac atcaagtcac tattgtacag cagaaagaca atttagattc ccacttgaac
    19921 ttggaaatca aaaaccaatg acagcccagt ggacagttac aggagcagga agtgtattat
    19981 tagctcctaa tggagacggt ccaaaagtaa agtatgttac tgttggtaaa gtaatagatg
    20041 agggtattga tgatggtaat aatatgggac cagctatggc tccagcagca atagatacta
    20101 tctactctta ctttgaagat actaatgatg acccaaatag ctttgatata attgctactg
    20161 gagatttagg tactcttgga aaacagatag cagaagactt tctaaaagaa aaaggtgtag
    20221 atatatcaaa agtttatact gactgtggta tagagatgtt taaccttaaa gaacaagatg
    20281 tccactgtgg tggaagtgga tgtgggtgtt cagctacagt gtttgcagga tatatatatg
    20341 ataagttgag aaaaaaagaa tttaacaaag taatgcttgt atcaacagga gcactacttt
    20401 caccgacaag tacactacaa aagcagacaa taccatgtgt tgcacatgca gtagtaatag
    20461 taaatgagta ggaagtgata taatatgtta atggactatg taagagtatt tattgtaggt
    20521 ggaattatat gtttaatagc ccaagtaatg atggattatt tcaaaatgca aactccatat
    20581 gttatggtaa cgtatgtaac aactggtgta gttttaactt ttctaggact ttatgagcca
    20641 ttagttaagt ttgcagcagc aggtgcaaca gttcctataa taggatttgg atattcactt
    20701 gctacaggag taataaaatc aattcaaagt gatggttttt taggtatttt tttaggaggt
    20761 acaactgcta cagctggtgg aatagcagca gctatattct ttggatatat tatgtcagta
    20821 gtatttactc caaaagcaaa accataaatc tttattaaaa gaataataag tttttgaaat
    20881 aataactttt tataaagcag gtggatatct tttagattcc acctgttttt tatataaaga
    20941 agcgtttaca taattgaagt tgacaattat ttacaacata gtgtagtatt atattatcaa
    21001 aataaaatga agtgaagtag gggataaaga tggtaactat taaagatatt gcaaggaatc
    21061 taggtatcag ttattcaact gtttctaggt gcttaaacaa taatcctaat gtatctgaaa
    21121 aaacaaaaaa gaaagtggta gaagaggcaa atagactagg gttccatttt aatgtaaatg
    21181 caagaaattt agcaaaaaaa gaaactaaca ggataggggt aatcttttca aataatttta
    21241 atcatcaaga tactagaaag ttttttagtg atataatgga tagttctata aattctattg
    21301 agactaataa atacgatttt ataatacagc ctaataataa cattagtggt gatagtaatg
    21361 tatataaaat ggtaaatgga caaatggttg acggattagt aattgtgtct caatcaataa
    21421 aaaaagaaga gtatgatttt ttaaaagaca ataattttcc acatgtattt atatatttta
    21481 aaccatcttt tataggagaa gtagataatt tcttttggga cgataacgtg tatggaggtt
    21541 atatagctac caagcattta attgaccatg gtcataaaga tataataaca gtaacttcag
    21601 atgataagag ttcaaaaatg catgaagata gaaccaaggg atatttgaat gctatggaag
    21661 aagcaaattt aaagacagaa gtcattaaat ctaaaatgga ttttgaatct caagttgaat
    21721 ttttagaaaa atacattgat aaaataaaaa aagcttcagc tatatttgta caacaagatg
    21781 taccagcgat ttctataata caagagttaa aaactactta tggtataaat gtaccagaag
    21841 atatttccat aatagggtat aataatattg aattaatatc atattttagg tcgcatttat
    21901 caacaataga tgacccaaga gagcaagtta taaaaaatgg agtagatagt ttagtaaata
    21961 taataaataa aaagagtaca gaacacattc caagaaaatt atatccaagg ttaataatac
    22021 gaaatagcgt taaaagaata aagtagtaag cttttaagta tcaaacgttt attaaaaata
    22081 ttatagatta tgtgaagttt tttaacaaat aatataaata aaaattaaaa ggagtaggtg
    22141 ta
//
LOCUS       DAYAOY010000065        22045 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYAOY010000065 DAYAOY010000000
VERSION     DAYAOY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.065"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            107..1240
                     /locus_tag="ACGI6B_002546"
     CDS             107..1240
                     /locus_tag="ACGI6B_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9751982.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            2156..3178
                     /locus_tag="ACGI6B_002547"
     CDS             2156..3178
                     /locus_tag="ACGI6B_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9751983.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3474..4394
                     /gene="pyrB"
                     /locus_tag="ACGI6B_002548"
     CDS             3474..4394
                     /gene="pyrB"
                     /locus_tag="ACGI6B_002548"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFG9751984.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4433..5125
                     /locus_tag="ACGI6B_002549"
     CDS             4433..5125
                     /locus_tag="ACGI6B_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFG9751985.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            5118..6020
                     /locus_tag="ACGI6B_002550"
     CDS             5118..6020
                     /locus_tag="ACGI6B_002550"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFG9751986.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            6105..6689
                     /gene="pyrE"
                     /locus_tag="ACGI6B_002551"
     CDS             6105..6689
                     /gene="pyrE"
                     /locus_tag="ACGI6B_002551"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFG9751987.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6903..7829
                     /locus_tag="ACGI6B_002552"
     CDS             6903..7829
                     /locus_tag="ACGI6B_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HFG9751988.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7881..8771)
                     /locus_tag="ACGI6B_002553"
     CDS             complement(7881..8771)
                     /locus_tag="ACGI6B_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9751989.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8959..9990
                     /locus_tag="ACGI6B_002554"
     CDS             8959..9990
                     /locus_tag="ACGI6B_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFG9751990.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            10284..11159
                     /locus_tag="ACGI6B_002555"
     CDS             10284..11159
                     /locus_tag="ACGI6B_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFG9751991.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            11177..12667
                     /gene="cls"
                     /locus_tag="ACGI6B_002556"
     CDS             11177..12667
                     /gene="cls"
                     /locus_tag="ACGI6B_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFG9751992.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12860..13144
                     /gene="groES"
                     /locus_tag="ACGI6B_002557"
     CDS             12860..13144
                     /gene="groES"
                     /locus_tag="ACGI6B_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFG9751993.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            13177..14805
                     /gene="groL"
                     /locus_tag="ACGI6B_002558"
     CDS             13177..14805
                     /gene="groL"
                     /locus_tag="ACGI6B_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFG9751994.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            15067..15807
                     /gene="larB"
                     /locus_tag="ACGI6B_002559"
     CDS             15067..15807
                     /gene="larB"
                     /locus_tag="ACGI6B_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HFG9751995.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15942..17074
                     /gene="larC"
                     /locus_tag="ACGI6B_002560"
                     /pseudo
     CDS             15942..17074
                     /gene="larC"
                     /locus_tag="ACGI6B_002560"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      17134..17235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17456..18991
                     /gene="guaA"
                     /locus_tag="ACGI6B_002561"
     CDS             17456..18991
                     /gene="guaA"
                     /locus_tag="ACGI6B_002561"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFG9751996.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(19068..20114)
                     /locus_tag="ACGI6B_002562"
     CDS             complement(19068..20114)
                     /locus_tag="ACGI6B_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9751997.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(20191..20406)
                     /locus_tag="ACGI6B_002563"
     CDS             complement(20191..20406)
                     /locus_tag="ACGI6B_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9751998.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            20692..20823
                     /locus_tag="ACGI6B_002564"
     CDS             20692..20823
                     /locus_tag="ACGI6B_002564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9751999.1"
                     /translation="MNENENMSLYVSKLKEVLKNNSEEYKKIRAKINEIYNLNEKKE"
     gene            complement(20840..21241)
                     /locus_tag="ACGI6B_002565"
     CDS             complement(20840..21241)
                     /locus_tag="ACGI6B_002565"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752000.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            21427..21618
                     /locus_tag="ACGI6B_002566"
     CDS             21427..21618
                     /locus_tag="ACGI6B_002566"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752001.1"
                     /translation="MRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEIAHKISLE
                     TGKTINEIFFDFTVNHSKL"
     gene            21858..21995
                     /locus_tag="ACGI6B_002567"
     CDS             21858..21995
                     /locus_tag="ACGI6B_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752002.1"
                     /translation="MQREQTTLRIPEDLHKALIDLSSDIGMPITSIIIIACWLYISKI
                     N"
BASE COUNT         8694 a         2286 c         3898 g         7167 t
ORIGIN      
        1 ctgttacaaa aaatgtatct ttttaattaa taaagataat atgaaaactt aaaaggcaaa
       61 taaacttaaa cataaaatac tcttaaataa gataggagaa aaatgtatgt ctaactttat
      121 tctaaatggg atttttagaa cagttatagt aagtagtcta agcattctta tgatattgat
      181 tttcaagaaa aatgtattta aaagatttag caaaaaattt aattattata tatggatgat
      241 agttgttatt aaattatttt taccatttac atattatact tttacaataa atatcttgag
      301 aagtaaaaaa cacattaata ttaataacat aaatttagag ggatttaata atgtttcaac
      361 tataagtaat aatattatat tgtacacttg gattataact gtagtagttt atttaattta
      421 cactatattt aaatatataa aacttaaaaa tttaataaat gacttatctt atgatgttga
      481 tgatggagag attgtaaatc tttataaaaa tatattagaa gaatttaata taacaaaaga
      541 tataaaactc aaatactctt atgaagttga aacaccagca ttttttaatt catgtgtgct
      601 tttgccacca catgagtata aattgaaaga gttagactgg atttttagac atgagcttat
      661 gcattttaag agtagggacc tttatttaaa atatgtaatc ctgtttttaa aaacagtata
      721 ttggtttaat ccatttatat atataatgga taaacatata gatttagatt gtgaacttta
      781 ttgtgatgaa agagttttaa aaaatcgaaa ttcagaggaa aaaaaggatt atgctttaac
      841 aattctaaat gcaatgagaa aaggttcaaa tacttcaaat aaatttatag ctggactcca
      901 taagcaatct gatattaaaa agagagttat gaatatgttt aatgaaaagt acaagaatgg
      961 tatattaatg gcattaactt tatgtttatt atcttctatt acttttttga aggttaattc
     1021 aacgagtatt gttaatctta atatattgcc aaaaggaatg gaagaaaaag atgttactaa
     1081 tagtgcaatt gttacaatga tagaatttac atatgcagaa gctcctaaaa gtatgagaga
     1141 agaacataaa ataaattgtg aattcttagg tttaatacct aaagactctg ataaaatagg
     1201 tgttggtata gaatattatg acagtctatt gaatgattaa ataaatatta ttttaacaga
     1261 tattaattat aaagttttga atagtaattt gttgagaaat aaatatatcc ttataaaaat
     1321 agatacttat ataccaagaa taaaaaatag tcaagatagt acacaaggat atacttataa
     1381 agaactcaaa taagtgaaat aaattattaa ttataaggtt tataatttta aaaaattaga
     1441 taaattcagg tgtaaaatac tataaaacac aattcggaaa agtgtatgtt gaacttcatt
     1501 taaaatggaa tttcaaagtt gctatagtaa gtagtttaag cattcttctt atattaatct
     1561 ttgagaataa tgtatttaaa aaagttagta aaaaatttaa ctattgtata ttaatgatag
     1621 ttgctattga tatattatct aaaaaaatta agtatgaaaa gattactgct aggacaatct
     1681 tttcatactt aatttttaat atttatatta tccaaaattt tcatcttttt aacttagaat
     1741 ttgagaatca ctattaggaa ataggctttg atttatgtag ggttaaaaat ttcacagaat
     1801 atctaacttt atactaaatc catcaaaaaa tatacgccat ataaaagcgc ataaaaaaac
     1861 acataaaaca atatttatgc gcactttaac ctcgaatggt acaaaattct ttacttttac
     1921 agacccatat tatataatta agtaaataat gtatatttag gtgacatatt ggataagcta
     1981 atttatttat tttattaata taatgatact ataaaagtta tataaagtgg tttacaaaaa
     2041 agttacagat agagctttta ttaatgtatt gtgtattagt attttatcct taggtacact
     2101 tcacctacaa aatagaatca taagataatt atgggaaaat tttaggaggt ttatcgtgaa
     2161 gaaaaagata ttaataccag tatttgcatc agttatggca ttatcagtaa gttcaattgt
     2221 aaatgcagat gaagtaaatg attcatctca aaataaggat gataaaacaa atactgaatt
     2281 aaatttaggt gaatataaag aagtaaaata taaagttgca aaaataaaag atggagtagc
     2341 tataaagata agagaagaag gtcaagttca aaacatagct tattctggag atgaatttac
     2401 ggttttagga actcaaggag aatgggttaa ggttaaagtt gaagatggcg aaggttggtt
     2461 agctactaga tatgtagata tatctgaagg tgtaggatat actaacgctg ataaagtaaa
     2521 cttaagaaaa gataagagtg aaagttcaga agtaattgaa gagctggaaa aaggaagttc
     2581 tttattggtt ttggaagata atggagattg gttaaaagtt aaagatgggg aaacagaagg
     2641 atatgtaaaa tcatcttata tatctgataa agctcctgta atagaagagc cacaagtaaa
     2701 cccaagtatt gaccaaaatg tagattctac aaataataat caacaaaaca atgcaaatac
     2761 taatcaacaa aataataatt caaatgtgcc aacagctaac tctaatgcag ttcaagctgt
     2821 acttaacttg gcatattcta agcaaggttg tccatatgtt tggggagcag aaggaccaaa
     2881 cacttttgac tgttctggat ttacacaata tgtgtataga aatgcagtag gaaagaatat
     2941 accaagaaca tctaaagcac aatctaaata tggacaaact gtaagtaaag caaatttaca
     3001 accaggagac ttagtattct tcacaactaa tggttctggt tctgttagtc atgttggaat
     3061 atatgtaggt gggggtaata tgatacattc accatcaaca ggaaaaacag ttagtgtaac
     3121 tagtataaat tcatcatact atacagcaag atttgttaca gcaaaaagaa tattataatc
     3181 ggtttgtaat atgacaaatt gataaataca taaatatata taaacgctct atattatatt
     3241 taatttaatc atggaaacaa aaaatgatta tattttaaat gtactataga gcgtttttgt
     3301 atcactttaa atctaatcaa gattcaagtt aatatgactg ttaaatagga gccttttcat
     3361 atgatgatta tttgcgcaat cccttgacag gatagggcaa aggtggtaag ctactaacag
     3421 tacaaaaata cttttaattt tagttcagag aggctaaaaa aggaggacga aagatgttaa
     3481 aatcaagaaa tttaatccag ccagaagact tttcaataga agaaatagat gaaatattag
     3541 aattagctca aaaaataatt gacaatccgt caaaatattc tcgtatatgt gaaggaaaac
     3601 tattagctac attattttat gaaccatcaa caagaacaag attaagcttt gaatctgcta
     3661 tgaacagatt aggaggacgt gtagtaggct tttcagagcc aaattcatcc tctgcatcaa
     3721 aaggtgaaac tctaggagat acaatgagga tagtatctgg ttatgttgat ataatagcta
     3781 tgagacatcc tcaatcaggt gcagcttcag aagcagctag atatacggaa gtgccattta
     3841 taaatgcagg agacggcaaa aaccagcatc caactcaaac acttacagac ttacttacta
     3901 taaaatcatt aaaaggaact ttagaaagcc atacaatagg attatgtggt gatttaaaat
     3961 atggaagaac tgtgcattct ttggttaagg ctatggcaag atataaaaat acaaagtttg
     4021 tatttatagc gcctgaagag ttaaaaatgc cagattatat aaaagaagct ataaagggac
     4081 atgcatatta tgaaactaat aatttggatg atgttatagg aagcttggat gttttatata
     4141 tgactagagt tcaacaagaa agatttgaag ataaatcaga gtatgaaaga cttaaaaact
     4201 attatatttt aaataaagct aaattagaaa aggcatctaa agatatgtta gtaatgcatc
     4261 cattaccaag agtaaatgaa atagacatag atgtagatag tgatgataga gctgtatatt
     4321 ttaaacaagc taaatatgga atgtatgtta gaatggcttt aataataaaa cttttaggta
     4381 taaatgaaga ctaatagtta tcatataata gtaaaacaaa ggagattttg ggatgtataa
     4441 aattctggaa aatatatata taggcgaaga tatgtataga atgaaagtaa aaggtaattt
     4501 tgaaggtaaa atgggacaat tctatatgtt aagagcatgg gatacttatc cagtactttc
     4561 aagacctata agtatacatg atatagatga agaagggata acttttcttt ataaagtagt
     4621 aggagaagga actcaaattt tgagtaacct aaaggtaaat gataatataa aattagaagg
     4681 cccttatggt aatggatatg ctaaggttga tggaaaggta gctctagtag gaggaggaat
     4741 cggtgtagct ccactatatt tggttgctaa aaatataaaa aattgtgatg catatcttgg
     4801 gtttagagaa gatgttatat tagaagatga atataaacaa gtatgtaata aagtctatac
     4861 aacagtagga aatacttttg taacagatat aattgatgtt gagaaatatg attatatatt
     4921 aacatgtggt ccaactccaa tgatggaaaa attagttaag atggtagaag gtactaaaac
     4981 gagaattatg gtatctcttg aaaaccatat ggcttgtgga gtaggtgcct gtttagtatg
     5041 tacgtgtaaa actaatggag gaaataaaaa gacttgtaaa gatggtccag tgttctgggg
     5101 agaggacgtg atttttaatg ggtaatttaa gtgtgaaatt tggaagtgta gaatttaaaa
     5161 atccagtaat aatggcgtct ggaacttttg gatttggaaa agaatataat gagatttatg
     5221 atatacaaaa acttggtggt ataagtagca agggtcttac tttaaataaa aaacctggta
     5281 acaatggtat gagagttcat gagacatcat ccggtatgat gaatagtgtt gggcttgaaa
     5341 accctggagt tcaaggattt atagactatg aattaccttt ttttagtaaa ctagacttag
     5401 tcagaatagc taatgttgga ggtgggacat tagaagacta cttattaggg gttcaaatgt
     5461 taaatgacaa gcctatagac attattgaat taaatatatc ttgcccaaat gtcaaagctg
     5521 gtggaatggc ttttggtata aaaaatgaag tggcaagaga agttgttcgt gaggtaagaa
     5581 acataacgaa actgccttta gtaataaaat tatcacccaa tgcagaagat attgtaggaa
     5641 tggcaaaagt ttgtgaagaa gaaggtgctg atggagtttc attagttaat acttttaaag
     5701 caatggcaat tgatataaaa aatagaagac cagtatttga aaatgtatat gctgggttat
     5761 ctggaccagc tataaaacct atagctttga gaatggtaca tgaagtgtgt aaaaatgtaa
     5821 atatacctgt aatgggaatg ggcggaataa ctaaagccac agatgctata gagtttataa
     5881 tggcaggagc tacttgtatc caagttggaa ctgcaaattt tatgaatcct agaataggta
     5941 ttgaaataat agatggaatt aatgaattta tggatagaga aggaataaaa agtttagacg
     6001 aaataagagg aattatataa ggtagaataa atagtatttt aaagataaaa aatatagatt
     6061 aaataaacaa taattttata acattaacat ggaggaaaaa taatatgagt aaagtagatg
     6121 tagtggatat attaaagaaa agtgatgcat tgttggaggg acatttttta ttatcttcag
     6181 gaaaacacag taatagatac gtacaatgtg caaaagtatt aagatttcca gaatatgctg
     6241 ctgaggtatt aagtacagtt gttgaacaaa taaaagactt agatatagac ttagtagtag
     6301 gaccagctat gggtggagta atagtttctt atgagttagg aagacaatta ggaaaagaag
     6361 ctgtatttac tgagagaaaa gacaatacaa tggagttaag aagaggattt gaagttaaaa
     6421 aaggagcaaa gataataatt gctgaagacg ttgtaactac tggtaaatca actatggaga
     6481 caaaaagagt attagaagcc ttaggtggag aagttgtagg tgttgcatgt atagcagata
     6541 gaactaatca tgatataggt atgcctatat atagtgctat aaaacttgat attcaagttt
     6601 atgaatctga tgagtgtcct ttatgtaagg aaggaaaatt accagttgtt aagcctggaa
     6661 gtagagagtt caaggaatta gggatgtaat aaaaacttaa ttatttatag tgttacttaa
     6721 aaaatgtaaa ttttttagag taaaaatttg taatataaca tataacttaa aaataaaaag
     6781 cagttagcct tataaatgaa ggttagctgt ttttttgtta aaaatatatt tacaaaataa
     6841 gagatagtag cagtacaata taagtataag gtaaaagtat tatatattag ggaggggtaa
     6901 gtatgaaaaa tcaattaaat gaaaatcata aaaaggttgt attttttgga gtaggtgctg
     6961 taggagctac ttttgcagaa caatttttta actctaaata tgattttaaa attctttgtg
     7021 ataatgaaag aaagaaaaga tatttagaag aaggatttat aataaatgga aaaaggtatg
     7081 attttgatta tgtaactaaa gatgagtata aacaagaggc tgattttata attataggtc
     7141 taaaatataa taatttaaaa gaaaatataa aagaattaga tggattagtt ggaaaaaata
     7201 cagttataat gtctctgcta aatggagttg atagcgaaga gataatagga gaaagatttg
     7261 gaattgaaaa aatggtatat tcatatgtta ccaatataga tgcaaagaaa atcaataata
     7321 atattataca cactactaat gggataattg tatttggtaa caaagataat agtgaagata
     7381 gaaaaactaa tataataacc gaagtctttg atgatgtaaa tatagaatat actttatcaa
     7441 aagatattca acgagatatg tggtggaagt acatggttaa tattggtgta aatcaaactt
     7501 cagctatact tggtgcacct tatggagttt ttcagagttc tgagcattta agagaattag
     7561 caaaatctgc aatgagggaa gttgttgcta tagcacaagc aaaagacata tctcttacag
     7621 aagatgatgt ggaacattca ttacatagaa tactagaaca ttcaaaagaa ggaagaacat
     7681 caatgcttca agatgtggaa gctcatagac ttacagaagt agatatgttt tctaagaata
     7741 tctgcaaact tggaaaaaaa tataacatac ctactcctat aaatcagact ttcttttata
     7801 tgataaaagt aattgaaagt agattttaaa gggtctttgt aagaaatatt gataattatt
     7861 gattttaaat tttattaatg ttatatacta tttaagttac taaatatttt taactcttca
     7921 atgaaaatag ttgctgcttt tgataaagtt atatcttttg gaaatatata tgagaaatgt
     7981 ctgttgtagc tgcttccaag ctctatgaca gacagctcat tgtttagaac aggtaagctt
     8041 gtcactagat tagagactat tgtaattcct aaattatttc taacagattc tttgacagca
     8101 taattacttc caaaaaccat catacttttt ggaataattt ctttaacact taaaaacata
     8161 tctaaataat ctctggttcc agaaccatcc tccctgacta tccatttttg gttttgaagt
     8221 ttatcaaaac taaaatcttt taataaatga ctcttgtatg gaagtgctaa aaccatctta
     8281 tcttcaaaaa aatattcttg aataaatgaa ggggaagagc aagtgccttc aataagacca
     8341 atgtctaaaa tataatcctt aacatgagaa gatacgattg atgtattttt tataaaaact
     8401 tctacatcta tgtcaggata ctttttagaa aataatgcta gaaaatttgg taaaatatac
     8461 tctccaatag ttaaacttgc accaatcttt aagtgaccag ttatagcatc tgaaacttgg
     8521 cttacatcat gataagtaat atttaaaaga tttaatattt ctttagctct tttgtacaag
     8581 atttgtccac tttctgttat aaaaatggtt ttttgtttaa tagacctgtt tataagcaca
     8641 actttaaaat aattttctag attttttatg tgtttactta cacttggttg tgatagattt
     8701 aaatattccc cagctttagt aaagtttttg tattcaacaa cagctacaaa tgtttttaat
     8761 tcttcaaaca attttttgtc tcctttacct aatgaaattt tacatttttt atatactttc
     8821 tacagataat tatactatat ataataacca ttaaaaacat taatcattac gatattatat
     8881 atttatttta cttatggtta ttacaattat atactattgc tatgaagaaa actatattaa
     8941 ttataaggta ggtatattat gttaaagaaa aaaagcagtt tgacattaaa aactataagg
     9001 gaaatattac caggactgtt tgtatcagta ttagtaggtt atattagtat atttatttct
     9061 attttaattc ctaaggtagg ggcagcatca atttctatat ttttaggtat gtttgtagga
     9121 aacctatttt taaatcaaaa ggtatttcaa aaaggttata aattttcaga gacagatttg
     9181 ttatcatatt caatagtact attaggagca acactcagtg tatctacctt aatagactta
     9241 aaagtttcag gaattttatt tataatattg caaatgacaa taacaataat agctgcactt
     9301 tatataggta aaaaactagg atttgaagaa aactttagat ttctaatggc aagtggaaat
     9361 gcagtatgtg gttcttctgc aataggagca acagttccag tggttaatgc gacagataaa
     9421 gaaaagggaa ttgcagtaac tattgtaaat gttactggta tatttcttat gtttttactt
     9481 ccaataatat ctcaattttt atatagccat gaacttgtaa aaacttcagc aatgataggt
     9541 ggaactttgc aatcaattgg tcaggttgta gcaagtggag caattgttgg agaaaatgta
     9601 aaagatttgg ctactatttt taagatagta agagtaatat ttctagttgt tgttgtatta
     9661 gtatttggac atatgaaaaa tcaatctaat agggaaattc ttgaagaaga aaaggatgag
     9721 ataaaaaaga aaaaagtgac tataccatgg tatgtaattg gattctttat aacttgtgca
     9781 cttttttcaa tgaacattat accaaaagaa gtatctgtgt tgtgcaaaga aataagtaac
     9841 aaactagaaa taatagcttt agcagcaatt ggattaaagg ttaatgtaaa agatttagtg
     9901 aaacaaggga aagaagtttc tttatatggt ttatttgtag gtatagtaca agttgtatca
     9961 gctgtagttt taataaaaat atttatatag cataaaaata gtatttgtaa tattaatttg
    10021 attaactaaa ataatatgat actaataatt tataatataa ataaaaaatg aatttcgata
    10081 agtttaagtg ggtataaact tatagaaatt cattttttat ttaaggtaaa aatttaaagt
    10141 aaaatattat tttacataat aataacctta taaattttga aattacttat ctttgtgtta
    10201 aaataagtaa aatgcacata ttagagctaa gtttttaagt aaataaacaa aatgaaaata
    10261 taaatattaa ggagatttgg tatatgaatg tttatgaaaa aggtaatgga gttatactag
    10321 aaggagtaac agattttgac ccagtacata tatttgagtg tggtcaatgt tttagatggc
    10381 acaaacaaga agatggctca tacactggag ttgcaaaggg aagaatatta aatgtcaaaa
    10441 aagagaatga taaaatatat ttaaataata ctaatttaaa ggagtttaac agcatttggt
    10501 ataattactt tgatttagga actgattata cagaaataaa gaataagcta aaaaatatgg
    10561 atgaatattt aaataaggct actgaatttg gatggggaat aagaatatta agacaagatg
    10621 gatgggaaat gcttatatca tttataattt cctctaataa taggattcct atgatacaaa
    10681 gagcgataga aaatttatca agaaagtttg gtaagtatat aggtgaatat gaaggtaatg
    10741 aatactatgc attcccaaca ccagaagaac taaataaggc ctcacaagag gaaataagag
    10801 catgtcaaac aggttttaga gataagtaca taaaaagtac tacacaggca gttatagaga
    10861 ataatgatga ggtatctgaa tatacaaatt taagtacaga ggattgtaga aaagagcttt
    10921 taaaatttaa tggagtagga ccaaaagtat gtgattgtat tgcattattt ggtatgcaaa
    10981 aatatgattc ttttccagtt gatgtttggg ttaagagagt tatgcaagaa ttttatatag
    11041 atgaagacat gagtttacct aaaatgagaa cttatggaat cgataaattt aaagaaatgt
    11101 ctggatttgc tcaacagtac ttattttact atgcaagaga actcggtata ggaaaataaa
    11161 ttaaggggga gaaattatgg gagtaatagg aacaatattt ctgttctatt taattatatc
    11221 atatctagct ggagccataa tttctgtgat tatattgctt gaaaatagag accctgcaaa
    11281 aacaatgtct tggctattga tgtttattat atttccaggt gtaggtttaa tgatatatgc
    11341 tatttcaggt agaaatatta gaaaaagaaa attatttaag actcaaaaac tagctaataa
    11401 tataaaagaa aagaaactat ttgatacatt agaaaagata acagaaatag ttgaattaga
    11461 aaaagaatct ataaaacaaa ataagctttt aagagatgaa gaggatggta gttatagaaa
    11521 aagggtaata aatatgttgt taaagactgg tatgtttccc tttactaaaa ataataaagt
    11581 ggatgtattt gtagatggaa atgaaaagtt taaaagactt atagaagata taagagaagc
    11641 taaagaccat attcatttag agtactttat catcaaagat agtgaaatag gtagggtgtt
    11701 aaaagaagaa cttataaaaa aggctaaaga aggcattaag ataagaattt tatatgatga
    11761 tgtaggatgc tggagatttt ggtttaatag aaaatttttt cgagaaatga gggaagtagg
    11821 tatagaaatt gcagcatttt tacctacaaa atttcctata ataggaggga aattaaacta
    11881 tagaaatcac agaaaaattg ttgttattga tggaataatt ggatatacag gtggaataaa
    11941 tataggagat gaatatttag gcaaaaatga taagtttgga tattggagag atactcatat
    12001 tagaattaag ggtatttctg tatacatgct tcaaatgaca tttctgattg attggtatta
    12061 tacgacaaaa gaagtattag tgactaaaaa ttatttccca agtgtaggga atgttggtga
    12121 aagtatgata caagttgttg cgagtggtcc agacagtgac tgggaagata ttcattatgc
    12181 ttatttttct gcaatatgtc aggctagaaa aaatgtttat atagaaactc catattttat
    12241 tccagatgaa agtctactaa aagctataaa gagtgctgct cttagtggag ttgatgtgag
    12301 aataatattt cctaaaattg cagaccacaa gatagtaaat atagcatctt attcatattt
    12361 tgaagaaata ttaagagcag gtggtaaagt ttatttatat aataaaggat ttatacattc
    12421 taaagtcgta ataatagatg ataaaattgc ttctgcaggt acagctaata tggatttaag
    12481 aagttttatg cttaactttg aggtaaatgc atttatctat gatgaagaag ttattagggt
    12541 aatgacagat gatttctttg aggatttaag tcactgtgaa gaacttaatt tggaggtatt
    12601 taagaataga aatatcatac aaaaaatcaa agaatctgta gcaagattat tttcaccaat
    12661 attataaaaa attattaaaa aaggtattga aaattatgtg tgattggtat attataataa
    12721 ttgttagcac tctattagat agagtgataa taaataaaaa gtatatatat aaatctacat
    12781 atataagtag tattaattta aaaataacta aaaacatagg cgaactagtc tatacttaat
    12841 attttaggag gcgtgaataa tgaaaataag accattagct gacagagtag taattaaaaa
    12901 agtagaagca gaagaaaaaa ctgcaagtgg aatagtttta ccaggagcag ctaaagagca
    12961 acctcaaata gctgaagttg tagaagttgg accaggtgga atagttgaag ggaaagaaat
    13021 aaaaatggaa ttgacagtag gagataaggt tatattccaa aaatattctg gaacagaagt
    13081 taagatagaa ggacaagaat acacaatact aagacagagt gatgtattag ctgttattga
    13141 ataaatatag aataaattta ttaggagggg tttaaaatgg ctaaagaaat taaattttca
    13201 gaggaaacaa gaagagcttt agaagctggt gtaaataaat tagcagatac agtaaaagta
    13261 acattaggac ctaaaggaag aaatgttata ttagataaaa aatttggttc tccacttata
    13321 actaatgatg gagtaactat agcaaaagag atagaactag aagatagatt tgaaaatatg
    13381 ggtgctcaat tagttaaaga agtagcaact aaaactaatg atgtagctgg agatggtact
    13441 acaactgcta cagttttagc acaagctata ataagagaag gtttaaagaa tgtaacagca
    13501 ggggctaacc caatactttt aagaaaagga atacaaaaag cagtaacagt agcagtagaa
    13561 gaattaaaaa atcaatcaag aatagtagaa acacaagagg ctatatctca agttgcttct
    13621 atatctgctg gtgatgaaga agttggaaaa ttaatagcag aagctatgga aatagtaggt
    13681 aaagatggag ttataactgt tgaagagtct caaactatga atactgaatt agatgctgtt
    13741 gaaggtatgc agtttgatag aggatttgtt tctgcatata tggttacaga tgtagataaa
    13801 atggaagcag ttttaaacga cccatatata ttaattactg ataaaaaaat atctaacata
    13861 caagaattat taccagttct tgaacaaata gttcaacaag gtaaaaagtt attgataata
    13921 gctgaagatg tagaaggtga agcattatct acattagtag taaataaatt aagaggaaca
    13981 tttgatgtag tagcagttaa agctccaggc tttggagata gaagaaaaga aatgcttcaa
    14041 gatatagcaa tacttacagg tgctcaagta atatcagaag aattaggtta tgatttaaaa
    14101 gaagctgatt tatctatgct aggtagagct tcatctgtta aggtaactaa agaaagtact
    14161 acaatagtag atggttctgg agataaaaaa gctatagaag atagagtaac tcaaattaaa
    14221 catcaagtag agcaaactac ttcagatttt gatagagaga aattaatgga aagattagct
    14281 aaacttgctg gaggagtagc tgttgtaaaa gttggagctg ctacagaagt agagttaaaa
    14341 gaaagaaagt taagaataga agatgctctt aatgcaacta gagcagctgt agaagaagga
    14401 atagtagctg gtggaggaac tgcttttgtt agtgttatac cagcaatagg aacactaatt
    14461 gagagtttag aaggagaagt taaactaggt gctcaaatag ttaaaaaagc gttagaagag
    14521 ccattaagac aaatagctat aaatgctggt cttgaaggtg ctgtaattgt gcaaaatgtt
    14581 gttaattctg aagcagaaac tggatttgac gcattgaatg aaaaatatgt aaatatgata
    14641 gaagctggta tagttgaccc aactaaagtt agtagaagtg ctttacaaaa tgctgcatca
    14701 atagcaagta ctttcttaac tactgaatct gctgttgctg accttcctga aaaagaagat
    14761 gcaggaatgc caggaatggg tggcggaatg cctggtatga tgtaatttaa aaaatttaaa
    14821 atataaattt taatataaag aactatttcg acttagattt taaactctaa gttgaaatag
    14881 ttcttttgta tatatctaat ttttgtatat ctataaattc aatacacata tttagataat
    14941 cagtatctag tttgtaataa tattttagag tagtattgtt attttttagg ttatgatttt
    15001 agatatatat taattaaggt ataattgttg atagagaata aatcaaattt aaaaggatgc
    15061 gataatatgg acttaagaaa attgcttgaa gaagttaaaa atgaaaatat tgatatagat
    15121 tttgcgttag aaaagttaaa agatttacct tatgaagacc ttggatatgc aaatatagac
    15181 catcatagag aattaagaaa tgggtatccg gaagttattt attgtgaagg taagagtgat
    15241 gagcatattt tagggattat tgataaaatg aatcaaaaaa gttctaacat attggggaca
    15301 aggtgcagaa aagaaacttt tctgaaaatt aaagaaatct ataatcatgc tgagtacgaa
    15361 gaggcgtcta aaattcttaa gattcaaaat catgatatag agaatatagg aaaaggtaaa
    15421 attgtgattg ctacaggggg aacttctgat ataccagtag cagacgaagc ttatcataca
    15481 gctaagtttt taggtaatga tgttgataga atatatgatg ttggagtggc aggtatacat
    15541 aggcttttaa ataaaagaca taaaatagat agtgctagag taatagtagc cgttgcaggt
    15601 atggaaggtg ctcttgcaag tgtaattggt ggactggttg atgttccagt aatagctgtt
    15661 ccaacttctg ttggatatgg agcaaatttt ggaggattag cagcattgct agctatgtta
    15721 aatagctgtg catctggaat atctgttgta aatattgata atggatttgg agcaggatat
    15781 ttagcatcaa tgataaataa actgtaaata cattgatata aattaaacaa aactatataa
    15841 taaaatttaa tttctattat atttttacta ttaaaattga ggagacatat atgagtgaaa
    15901 aaatacttta ttttgatata ataagcggta tatcagggga tatgaccctt gcaagtttat
    15961 taaatttagg tgtgcctaag gaaatttttt tggaggaaat tcataaacta agaatgagcg
    16021 atgaatttaa tataaacatc agctcgaaaa ctgaaaatgg aatagtaggt acaaaagtag
    16081 aagtaattac aaaagaaaaa catacacata gaaatttagt ggatatattt gaggttatag
    16141 ataaaagcac tctaaacgaa aatgtaaaat ctaaagcaaa aaaaatattt atggtaattg
    16201 gagaagcaga agcaaaagtt catggaacta caatagacaa gatacatttt catgaggtag
    16261 gggctataga ttcaattgta gatatagtag gagcttctat attagttgac ttactttgtg
    16321 tagataaagt ttgtgctagt acagtgtctg ttggttcagg atttgtaaaa tgtgaacatg
    16381 gaattatacc tatacctgca cctgcaacta tagaaatttt aaaaggtgtg ccaattaaat
    16441 taaataatgt aaatggagaa tgtacaactc caacaggagc tgcaatcata aaagttttgt
    16501 gtgatgagtt tgtagatgag cttgaatttt ctccaaagca aattggatat ggtataggtc
    16561 ataagaagtt tgaagtacca aatatactca gaactgtatt aggtgaaaaa aaaaagaaga
    16621 attaatttat gaaattagtg caaacattga tgatatgtct tcagaaatct attcttattt
    16681 gtatgaaaaa atattagatg agaaagcatt ggatatttat acagagagca tttatatgaa
    16741 gaagaataga ccagcaacaa aagtatcaat tttatgtagt gaatcagatt tagaaaaatt
    16801 tattaaaata ttacttttag aaactagtac ttttggagtg agatataatg catataatag
    16861 agaaattcta gagagaaaat ttgtaaaatt agatacaaaa tatggagtgg ttacagtaaa
    16921 actagggtat tataaaggaa aactagtcaa ggctactcca gaatataatg aatgtaaaac
    16981 tatagcaaaa aatgaagata ttcctataaa aaaagtgttt gctgatataa attttagaat
    17041 aaaagaagaa tttagtgaaa acttattgac ataagtaata aattttgata aattaatatt
    17101 cagccttatt aaataaaagt aaacagaaaa aaatatataa aatataaact cactcgtata
    17161 tgctcagaaa tatggtctga gagtctctac caagataccg taaatatctt gactatgagt
    17221 gaaattatta taccaaaaga cctagttaag taaatgtgta ggctgagtta aactccctat
    17281 gtgttggcta taagataaag gtattttgga agcctgtata gtaatttcac ttttgctaga
    17341 aattatggaa gttactatac aggttttttg tgtaaaaaga tttaatataa tttagactgt
    17401 attgtgttaa aataaatata taaaaactat attatacaag gagagatgtg aatttatgaa
    17461 acatgaatta gtacttgtaa tagattttgg aggacaatat aatcagttga tagctagaag
    17521 agttagagaa aacaacgtat attgtgaaat acttccatgt actgcaagca tagaaagaat
    17581 aaaagaaaaa aatcctaaag ggataatatt tacaggcggt ccaaatagtg cgtatttaga
    17641 agattctcca acaatatcaa aagaaatatt tgaattagga gtaccaatac ttggaatatg
    17701 ctatggtatt caaataatgt ctcatgtatt gggtggagtt gtaagaaaag gtaataaaca
    17761 agaaaaagaa tatggaaaaa cagctataac ttatggaaaa tcatcattat ttgaaggaat
    17821 aactacaaat agtgtatgga tgagtcatac agatttaata gaaaaagttc cagaaggatt
    17881 tactatagtt gcaaatacta atgattgtcc agtagcagct atggaaaatg tagaacgaaa
    17941 cctatatgga gttcaattcc atccagaggt ggaacactgt ttggaagggg ataagatact
    18001 tacaaacttc ttatataata tttgtaaagt aaaaggtgac tggactacag attcattcat
    18061 agaagataaa ataaaagaat taaaagaaaa aataggcgat aaaaaagctt tatgtgcttt
    18121 aagtggagga gttgattctt ctgttgctgc agttcttatt cataaagcaa ttggggacaa
    18181 cttaacttgt atatttgttg accatggttt acttagaaaa aatgaaggaa atgatgtaga
    18241 aagaatattt agagagaaat ttgatataaa tttaataaga gttaatgcag aagatagatt
    18301 cttatctaaa ttaaaaggag tttctgaacc agaagctaag agaaaaataa taggtgaaga
    18361 atttataaga gtatttgaag aagaatcaaa taaattagga aaaatggatt tcttagtaca
    18421 aggaacaatc tatccagatg ttatagaaag tggacatgga aatgctgcta ctataaagtc
    18481 tcatcataat gttggtggaa tcccagaaga tgtagacttc caagaaattg tagaaccatt
    18541 aagagagtta tttaaggatg aagttagaaa aataggatta gagcttggta tagaagaagg
    18601 attaatattt agacatcctt tcccaggacc aggtctagga ataagagtta taggtgatgt
    18661 aactaaagaa aaatgtgata tattaagaga agctgatgct gtttatatgg acgagcttag
    18721 aaaagcaggt ttatacagag aaatatggca agcttttgca acattaccag atgttaagac
    18781 tgttggagtt atgggtgatg aaagaacata tgcttattta gttggtttaa gagcagttac
    18841 ttcttctgat ggaatgactt ctgactggta taagatgcct tatgatgtat tagaaagaat
    18901 ttctaataga ataataaatg aagttgatgg agtaaataga gtagtttatg atataacttc
    18961 taaacctcct ggaactattg aatgggaata gtaaataaaa aagtcgtaag ccttgtattt
    19021 tcaaggtttg cgacttttaa aaaaatcttt gtgatactaa aatgatacta tatatttaaa
    19081 ttatctagtt tttgggcaac ttcaagttgc ttatttgggt ataaatgtga atatgtattc
    19141 caagttgttt ctactttttc atgccccaat ctttctgcta tagttaatat atttacatcc
    19201 atatttacca ataatgacgc atgagaatgt ctcaaatcat gcactctaat tctttttaca
    19261 ttagataact tacagcatct atctagctct ttagaaagat aacttttagc aaatttaaat
    19321 attctttcat tatcttttaa gtcatatagc ttagatagat aatcttttat attattatat
    19381 aagaaatgtg ggatagggac aacacgttta cttttaggag ttttaggaga agaaacaata
    19441 tcctctccat ttagtcttat gtagcttttt tcgacgctta ttttattttc gaaaatatct
    19501 tttggagtaa gagctaacaa ctcccctagt ctaagacctg tccaaaataa aatttcaaat
    19561 gctagtctag gttctgattt cttttcaaat tcaataaatt ttttaaactc ttctaatgtc
    19621 caaaagttca tttcatctgc gtttttttta ccgatagaac ctgccaaatg agcaggatta
    19681 gaaggtaagt tataatattt aactgcataa tttaatacag caacaagctg gttgtttatc
    19741 gttttaatat atgtttggct ataatcactt tttaataatt cgttttgcca tcttcttata
    19801 tgtgttgcag taatttcatt tatttttaga cttttaaaaa aaggtaaaat tctaaagttt
    19861 attaaatact ttttagtttc taatgtagat aattttaatc tagaagacat atcgtgcatg
    19921 tattcttcta tgagactttc aaaactcata tcagtactca ttttagattt attcaaaaat
    19981 tctctttcaa actctagagc ttctttttta gttttaaaac ctctcttaat tttctttttc
    20041 ctatccccat cgaagtctgt aaaataaaag ctagcatacc aactttttct ttgttcgtct
    20101 ttgtaagcag gcattttaga cacccccaat tatgtcattt taatattaca ataattataa
    20161 catgttagat atgctatcta aataattgtt ttattttcct cttcttttta tttcttgaag
    20221 cttgtaatat ttacacagtt ctaagaagtc ttcttctagt gctatagata agtctaatat
    20281 cgtagatata cctatatctt tgtactcttg attttctagt tttgatatat aacttctgtt
    20341 acgtctcatt ttttctgcta attctagttg tgtcattctt ttcttttttc ttaatttttt
    20401 caacatttat taaaatccac cttttaatta tttttatgac atttttataa ttaatagttt
    20461 gtgtaatttc ttgtaaaaaa tgttcctcat tagaacattt tttggtggaa aaatgtgtta
    20521 aaatgtaagt aagaaaagca ctttcaaagc actataaaaa tagtgctgaa atagataaac
    20581 aaataaaagt aataaggagg aaagatagat tctagaggga aatcattatc atttttaaga
    20641 acgtatgttc ggttggtggg atagaaaaat actttacagg ggatggttta tttgaatgag
    20701 aatgaaaata tgagtttata tgtcagtaag ttaaaagaag tattgaaaaa taattcagaa
    20761 gagtataaaa aaataagagc taaaataaat gaaatttata atctaaatga aaagaaagaa
    20821 taaaagggct ttagctcttt tattctttct tagtattaac tgctttataa taagcattat
    20881 ctatcatttt ttttattgca tttttatcat ctgaatctaa agatgctaat ctatttatta
    20941 gttcgttaac ttcattatct tcttttgagt taactttttt taataattca tcatcttgtt
    21001 gagtgttagg ttgtatttta cctaatttag ttaatagctc ctttaaagtt aaatttaatc
    21061 caaaactcat tttttcaagc atatctaaag taggttcaac agctttacca ttccttgggt
    21121 ctctgttttt ttctatctta tctatatatg tatggctcac accacaaaga ttagaaaatt
    21181 ctctaagaga taagttatgt tcacttctat attctttaat aatttctgct aatgtttgca
    21241 taatttcaca cctcatctaa gtaatagttg acttaattat atcagaaaaa tgtaaactat
    21301 tgttaacaaa aataaaaatt tagtaaaaaa taattgacaa gagtaaaaaa aagacgtata
    21361 ctatgattaa caaaaaggtt ataaataaag ttaggaggga ttaatataaa aaataattta
    21421 caatatatta gaaaagaatc tagaatatca caaaaaaaat tcgctgataa gataggtata
    21481 tcaagaccgt atttatctag aatagaaaac ggtaaggtta atccgagttt agaaatagcg
    21541 cataaaatct cgctcgaaac agggaaaaca ataaatgaaa ttttttttga ttttactgta
    21601 aatcatagta aactataagg ttttaattgt accttgaaaa ctaaatataa aatattttaa
    21661 aggagcaagt atatgaaaaa taaagaaaaa gaaataatca ttagaggaat tttaatagga
    21721 attttttatt ttttaggaat tgtatttagt aatacttttt ttaaataata aatagaaggg
    21781 gtaggtgaaa taccaactcc ccaaatcaaa ataacacttt ggaagttaaa tataaaatat
    21841 tcaaaagagg tgattagatg caaagagagc aaacaacgct tcgtatacca gaagacttgc
    21901 acaaagcgtt gatagattta agcagtgata taggaatgcc tattacatct attataataa
    21961 ttgcatgttg gttgtatata tcaaagataa attaaccaat ccgatatgca aaatgaatgc
    22021 acaacattat ttattagaga attga
//
LOCUS       DAYAOY010000066        21942 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYAOY010000066 DAYAOY010000000
VERSION     DAYAOY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.066"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI6B_002568"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI6B_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFG9752003.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="ACGI6B_002569"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="ACGI6B_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFG9752004.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI6B_002570"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI6B_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFG9752005.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI6B_002571"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI6B_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFG9752006.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI6B_002572"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI6B_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFG9752007.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI6B_002573"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI6B_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFG9752008.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI6B_002574"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI6B_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFG9752009.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI6B_002575"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI6B_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFG9752010.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI6B_002576"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI6B_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFG9752011.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI6B_002577"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI6B_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFG9752012.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI6B_002578"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI6B_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFG9752013.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI6B_002579"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI6B_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFG9752014.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI6B_002580"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI6B_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFG9752015.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI6B_002581"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI6B_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFG9752016.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="ACGI6B_002582"
     CDS             6989..7174
                     /locus_tag="ACGI6B_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFG9752017.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI6B_002583"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI6B_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFG9752018.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI6B_002584"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI6B_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFG9752019.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI6B_002585"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI6B_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFG9752020.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI6B_002586"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI6B_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFG9752021.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI6B_002587"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI6B_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFG9752022.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI6B_002588"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI6B_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFG9752023.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="ACGI6B_002589"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="ACGI6B_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFG9752024.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="ACGI6B_002590"
     CDS             11117..11767
                     /locus_tag="ACGI6B_002590"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFG9752025.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="ACGI6B_002591"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="ACGI6B_002591"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFG9752026.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="ACGI6B_002592"
     CDS             12536..12805
                     /locus_tag="ACGI6B_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HFG9752027.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="ACGI6B_002593"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="ACGI6B_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFG9752028.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI6B_002594"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI6B_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFG9752029.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI6B_002595"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI6B_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFG9752030.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI6B_002596"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI6B_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFG9752031.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI6B_002597"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI6B_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFG9752032.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="ACGI6B_002598"
     CDS             14901..15848
                     /locus_tag="ACGI6B_002598"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFG9752033.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI6B_002599"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI6B_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFG9752034.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="ACGI6B_002600"
     CDS             16330..17163
                     /locus_tag="ACGI6B_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFG9752035.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="ACGI6B_002601"
     CDS             17151..18017
                     /locus_tag="ACGI6B_002601"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFG9752036.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="ACGI6B_002602"
     CDS             18011..18814
                     /locus_tag="ACGI6B_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFG9752037.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="ACGI6B_002603"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="ACGI6B_002603"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFG9752038.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI6B_002604"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI6B_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFG9752039.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI6B_002605"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI6B_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFG9752040.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21056..21760
                     /gene="cwlD"
                     /locus_tag="ACGI6B_002606"
     CDS             21056..21760
                     /gene="cwlD"
                     /locus_tag="ACGI6B_002606"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HFG9752041.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT         8816 a         2563 c         4494 g         6069 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aaatttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa tagggacaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAYAOY010000067        21893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYAOY010000067 DAYAOY010000000
VERSION     DAYAOY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.067"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..140
                     /locus_tag="ACGI6B_002607"
     CDS             <1..140
                     /locus_tag="ACGI6B_002607"
                     /inference="COORDINATES: protein motif:HMM:NF019488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFG9752042.1"
                     /translation="YEVDLIRIKTKVKTIKISGDKLSLKYITDSEIGIKGIIYNVEYV
                     D"
     gene            153..1496
                     /gene="yqfD"
                     /locus_tag="ACGI6B_002608"
     CDS             153..1496
                     /gene="yqfD"
                     /locus_tag="ACGI6B_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HFG9752043.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1435..2550
                     /locus_tag="ACGI6B_002609"
     CDS             1435..2550
                     /locus_tag="ACGI6B_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFG9752044.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            2607..3068
                     /gene="ybeY"
                     /locus_tag="ACGI6B_002610"
     CDS             2607..3068
                     /gene="ybeY"
                     /locus_tag="ACGI6B_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFG9752045.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3074..3793
                     /locus_tag="ACGI6B_002611"
     CDS             3074..3793
                     /locus_tag="ACGI6B_002611"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFG9752046.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            3912..4313
                     /locus_tag="ACGI6B_002612"
     CDS             3912..4313
                     /locus_tag="ACGI6B_002612"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFG9752047.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4377..5270
                     /gene="era"
                     /locus_tag="ACGI6B_002613"
     CDS             4377..5270
                     /gene="era"
                     /locus_tag="ACGI6B_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFG9752048.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5282..6661
                     /gene="mgtE"
                     /locus_tag="ACGI6B_002614"
     CDS             5282..6661
                     /gene="mgtE"
                     /locus_tag="ACGI6B_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFG9752049.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6734..6889
                     /locus_tag="ACGI6B_002615"
     CDS             6734..6889
                     /locus_tag="ACGI6B_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HFG9752050.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            6855..7628
                     /gene="recO"
                     /locus_tag="ACGI6B_002616"
     CDS             6855..7628
                     /gene="recO"
                     /locus_tag="ACGI6B_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFG9752051.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7621..8220
                     /locus_tag="ACGI6B_002617"
     CDS             7621..8220
                     /locus_tag="ACGI6B_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HFG9752052.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8380..9258
                     /gene="glyQ"
                     /locus_tag="ACGI6B_002618"
     CDS             8380..9258
                     /gene="glyQ"
                     /locus_tag="ACGI6B_002618"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFG9752053.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9258..11324
                     /gene="glyS"
                     /locus_tag="ACGI6B_002619"
     CDS             9258..11324
                     /gene="glyS"
                     /locus_tag="ACGI6B_002619"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFG9752054.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(11490..13037)
                     /locus_tag="ACGI6B_002620"
     CDS             complement(11490..13037)
                     /locus_tag="ACGI6B_002620"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HFG9752055.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            13317..14084
                     /locus_tag="ACGI6B_002621"
     CDS             13317..14084
                     /locus_tag="ACGI6B_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFG9752056.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            14367..14564
                     /locus_tag="ACGI6B_002622"
     CDS             14367..14564
                     /locus_tag="ACGI6B_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9752057.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            14593..15669
                     /gene="vorB"
                     /locus_tag="ACGI6B_002623"
     CDS             14593..15669
                     /gene="vorB"
                     /locus_tag="ACGI6B_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9752058.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            15672..16391
                     /locus_tag="ACGI6B_002624"
     CDS             15672..16391
                     /locus_tag="ACGI6B_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9752059.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            16404..16943
                     /locus_tag="ACGI6B_002625"
     CDS             16404..16943
                     /locus_tag="ACGI6B_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9752060.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            17100..18158
                     /gene="buk"
                     /locus_tag="ACGI6B_002626"
     CDS             17100..18158
                     /gene="buk"
                     /locus_tag="ACGI6B_002626"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9752061.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            18176..19069
                     /locus_tag="ACGI6B_002627"
     CDS             18176..19069
                     /locus_tag="ACGI6B_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFG9752062.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            19099..20307
                     /locus_tag="ACGI6B_002628"
     CDS             19099..20307
                     /locus_tag="ACGI6B_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9752063.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            20331..21590
                     /locus_tag="ACGI6B_002629"
     CDS             20331..21590
                     /locus_tag="ACGI6B_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9752064.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         8580 a         2392 c         4102 g         6819 t
ORIGIN      
        1 aatatgaagt tgatttaata agaataaaga ctaaagttaa aacaattaaa atatctgggg
       61 ataaactttc acttaagtat attactgata gtgagatagg tataaaaggg attatctaca
      121 atgttgagta tgttgattag gaggagatac acttgataag ttttataagg ggatactatg
      181 ttatagttgt ggaaggagta ggactcgaac aatttttaaa tcatcttata agaaatggga
      241 taagtgttta caatgtaact agaataaaaa atactaaaat ggagtttcat atagatagac
      301 aagatataaa agactttaag aatgtttata gaggaagtaa gtttgatata aaggtaaagc
      361 aaaaaactgg ggtccctttt attatcaaaa gagtttataa gcacaaaggt atgtggattt
      421 gtgctcttgt ttctttattt ttattgatgt ctacatcaca gtttgtgaca gatgtatata
      481 tacaatctcc tgaaggtata aaaaaagaag ctcttagaaa tgagctttat aaggttggag
      541 taaggccagg agtctataag aagagtattg atagaaaaga agttagagac catatgatgt
      601 caaaatttaa tgatgtggca tatttatcta tcaatgtaaa aggaacaaat atatttgtta
      661 ctgttacaaa aaaggcagaa tccctaaaat ctacagacca atcaaattat tgtaatgtaa
      721 ttgctttaaa aaatggtata atagaaaagg taatccctag aagtggtaaa tcagttatta
      781 aatctggaga catagtccaa aagggagatg tcttattaaa tggagctaat accaagtcaa
      841 taccagaagt atgggcaagt actttttatg agtccacaaa aaaggcaagt tatgtagata
      901 ctgtaaataa aaaaactgga gaaaagaaaa atatatatac actaagtttt tatgataaag
      961 agtttacaat gagaaaaaat ataaaatata aagattatgt tgttgaaaat aaagaaaaaa
     1021 aactatcaat aggaaactat acttttccaa taaagataaa aacgagtact ttttatgaaa
     1081 caaaaaaggt tgaagtaaaa aggaataaag aagagttaaa aaaagaatta tctgaaaaag
     1141 ctttaaaaga gttagaatac ataatcccag cttcggctag aataatagat gtaaaacata
     1201 actttaaggt taataaaaat atgttagaat atttaataac agttcaaact agtgaaaata
     1261 ttgctaaaat ttatcctctt agtaagtcag aagcagaaag gtttataaag gaagaaagta
     1321 aaccagatga aggtgaagag gaagttcctt ccaatccaga aaaaagacca ttaaatgata
     1381 taagaaatga gtttgatgag gataataagg ataataaaga ccaaaacaat tctgatgaaa
     1441 ataatagtaa tcaaaatagt aataatagtc aaaataataa tagcaataac aactaataaa
     1501 attaaactta aaatcttagg aggactaatt caattgataa tacaaaagaa atttacaata
     1561 gcagatggta actttgaaag agagttattt ggcaactttg atgaaaatgt aaaattaata
     1621 gagaagactt taaatataga tgtaatactt agagaaggca atataatttt aattggagaa
     1681 gaaaaaaatg tagattctgc attaaaactt atgaatgaat tacaccaaac tgtatcaaat
     1741 ggtaagcatt tagataagca gagtatttct tattcattat ctcttttgct tgaaggaagt
     1801 gaacaaaaaa taaaagaact tgaggggaca atcgtcataa ctcaaagggg aaaagctgtt
     1861 caacctaaaa ctttaggtca aaaagaatat ataaaactta tagaaaataa tgatataact
     1921 tttggtgttg gacctgctgg aacaggaaag acttatcttg cggttgctat ggcagtaaaa
     1981 gcttttaaaa gagatgaagt tagtagaatt atacttacaa gaccagctgt ggaagcagga
     2041 gagagtctag gttttttacc aggagattta aaggacaaag ttgacccata tttaagacca
     2101 ctatatgatg ccttatttga aatgttgggt gctgataaat ttaataagta tttagaaaga
     2161 ggtactattg aggttgcacc acttgcattt atgagaggta gaacacttga taattcattt
     2221 ataatattgg atgaagctca aaatactact tctgaacaaa tgaaaatgtt tttaactaga
     2281 cttggatttg gctctaaagc tgtagttaca ggagatgtaa ctcaaacaga tttaccacaa
     2341 aataagaaga gtggtctaat acaagctaca gagattctta aaggtgttcc aggtattggg
     2401 tctataatgc taacagatag agatgttgta agacatgagt tggtacagag aataataaga
     2461 gcttatgaga agcacgacaa aagagaagaa tttaaaaaag aagaacgtaa aaaagcgaaa
     2521 atgcaggaaa agactttcaa aagaaagtaa aagtgtagta atatagatat atagcattat
     2581 taatgaataa aaagtaggtg tagtgaatgg atttgatact agatgataga caagataaat
     2641 tagaagtaag tgaggagtta attgaaaaga taaaggatat aatcatagaa tgtttagatt
     2701 atgaaggata tgatgataat tatgaagtga gtctttcttt tgtagataat aaagagattc
     2761 atgaattaaa tagagaatat agaggagttg atagagtgac tgatgtttta tcatttcctc
     2821 ttttaagtga tgattttgaa gatgttgagt tagaagaaga atccctaggt gacatagtag
     2881 tatctcttga aagagcctta gaacagagta tagagtataa ccatagtttt gaaagagaag
     2941 tatgtttttt aatatgtcat agtatgtttc atcttttagg ttatgaccac gatacagatg
     3001 aaaatacaaa agaaatgaga gaaaaagaag aacacatatt aaataagtta aacataacaa
     3061 gggagtagtt tttatgaaac cagaaaaaac tcgtcaaggt ataattaaag catttaatgc
     3121 agctatagaa gggatattgt atacatttaa atttgaaaga aatatgaaaa tacattattt
     3181 aggttcagtt gctgttctta taataagttt gttttttaac ttttcaaaat tagaaatgat
     3241 aatgttactt atgtccatat gtttagttgt agttgctgaa atgtttaata cagctataga
     3301 aaaagctgtt gatttagtaa ctgatgaata tcatgttctt gctaagatag caaaagatgt
     3361 tgcagctgga ggcgttctag tggcagcact aaattcagta gtagttggat atattttgtt
     3421 ttatgataaa ttgacagata tatctggaat attaatttat aaaataagag aatcagaatt
     3481 acatataacc ttgatatgta tattattggt tcttatagct gttgtagttg taaaagcatt
     3541 gacttctaca ggtactcctc taaaaggagg aatgccaagt ggtcatgctg ccttagcatt
     3601 tgctatagca actgcaatta cacttatgac tgaaagagtt gttgcctcta ctcttgcata
     3661 tataatggct gtattagttg cccaaagtag aattgaagga aaaatacata cattttggga
     3721 gactattgct ggagctttgt taggtgtgtt gatagctata ttagtatttc agcttggaat
     3781 gttctataat taaaataaat gtacataaaa tatttaaaat aaaacaaatc tgtgctaatt
     3841 aaatttttat gatataatgt agagtattaa ataaaaataa cttttaatta ttgtatacag
     3901 gaggagttgt tgtggataac atagaattgt taaggttggc agaagatgcc agacaacatt
     3961 cttatgctcc ttactcaggt tttagagtag gtgcagctct tttaactaag agtggtaaag
     4021 tttatactgg atgtaatata gaatgtgcat cattaggtgg aacaaattgt gcagaaagaa
     4081 cagctatttt taaagcaata tcagagggtg ataaggatat ctataaaata gctatagcaa
     4141 gtgataactc agaaaataat gagcaaacat atccatgtgg aatatgcagg caggtaatta
     4201 tagagtttgg ttctgatata aaaataataa cgggatatac taaaggagag gtgtttgagc
     4261 atagtataaa ggatttgttg ccaaactatt tctctggaaa tgatttaaaa taaatttaaa
     4321 agtataaata aatttaagta attaaaacaa gaagataaaa agttaggaga cagttaatgt
     4381 tcaaatcagg ttttgtcagt atagtgggta gaccaaatgt aggaaaatct actttaatga
     4441 ataatgtagt tggagaaaaa atagctataa tgagtgataa gcctcaaact acaagaaata
     4501 caatacaagc agtatataca gatgaagaaa tgcagatagt attcttagat acaccaggta
     4561 ttcacaaacc aaaaaataaa ttaggtgaat ttatggtaaa ggcagcaaca gaagctttta
     4621 aaaatgtaga tttaatatta tttgtagttg atgattctaa aaaaataggt cctggagata
     4681 gaaagataat agaggattta agaagtgtta agacaccaat aattttagtt gtaaacaaaa
     4741 tagaccaact agaccaaaaa gatgagctat ttgatataat caaaatgtat gatagagaag
     4801 gtatttttaa agaaatagta ccaatatcag cattaaaagg aaagaatacg gataccttaa
     4861 taaaagttat acaaaattat ttagaagaag ggccaaagta ttttccagat tatatgatta
     4921 cagaccagcc agaaagagta ttaatagcag aacttataag agaaaaagtt cttcactatc
     4981 ttaatgatga aatacctcat ggtgtagctg ttgaaattga aaaaatgaaa gcgagaaatg
     5041 ataaggaaat agtagatgta tctgctgtaa tatactgtga aagagattca cataagggaa
     5101 taataatagg aaaaaatggt aggaaactaa aaggaatagg taagtcagca agacaagata
     5161 tagaattgct tttaggttca caaataaatc ttcaattatg ggttaaagtc aaggaaaatt
     5221 ggagaaattt acaaaattat ataaataatt ttggatataa tgataaataa aggtgggtta
     5281 tatggatagg aaactggatg cttcccaaga tttattatat gaagtaaaat cattaattga
     5341 taataataaa gtattggagt taagagaatt aatagaggaa tatcatataa ttgatatatt
     5401 cgatataatg gaaaatttag aggaagacat gaaaattcaa ttatttgaag tacttccttt
     5461 agatatggca tcttctatat tagaagaagg tagtgtagag ttttttatta gtattttatc
     5521 taaactggat gtagaacact cgaaaaatat acttgaactt atgtctcttg gagatatggc
     5581 agacaagttg agtgaacttg aagaagaaga aagagaacac ataataaatc ttttgaatca
     5641 agagaatgcc gattatgtaa aagagttact attctatgat gaagattctg ctggtggaac
     5701 aatgaccact ggatatattt ctataaataa agatatgaca gctcttgagg ctatagacca
     5761 tatgagagaa gaagctgaag aagctgaaac tatttattat atatatgtgg ttgatgatga
     5821 agaaaaacta gttggagtat tgtcattaag agaattaata atagctagag acgcaaatat
     5881 agtagaagat ttgatgagtg aaaatataat ctctgtttat gtagatgaag atagagaaga
     5941 agcagtaaga ttagtatcta aatacaattt aatagcaatt ccagtagtag acagacaaga
     6001 aaaattaaaa ggaataataa cagtagatga tataattgat gtaatggaag aagaagcaac
     6061 tgaagatatg tataaatttg caggaagttc agaacatgaa agagaagttg cggaaaaaga
     6121 aaaccctaca ttgagagagc aaataatatc agcacttaga ggacgattac cttggttaat
     6181 tattacctta gttggagggt tgttggcttc gttaatactt tctaacttag actatataat
     6241 gaatcctgta tatgctccat tagtattttt tatacctgta gtaattggta tgggtggaaa
     6301 tattggaact caatcatcat ctgttacagt aataacattg tctaataagg atttaaattt
     6361 tagcaatgtg gttagagaag gtattgtagg tattattaca ggtcttttgt gtagtataat
     6421 aacaggtatt gtaatttact ttgttatgag aaagttagac attgtattaa ttgtttcaat
     6481 atcattattt ataaatatgg ttttaggtgc aacaataggt gcgtttatgc ctgttttatt
     6541 aaaaaaaatg gatgcagacc catctacagt ttcatcacca attatatcta cagctcttga
     6601 tataactggt atagcagttt actttataat aacaacggca ttattgtcaa aaattgttta
     6661 ataactttat gtatgattta tcctcttctt actaaaaata taagtataaa tgtaagaagg
     6721 gggtttttta aaaatgaaca atatcagttg ggaagttttt aaaaaaacag gtagtataga
     6781 tgcatattta tatttctgtg attgcaaaaa catacatgaa gaaattgaag aagtaaggga
     6841 gaaaaaagaa gacgatgata atcctgaaca cccaggggat agtacttaaa gctataaggt
     6901 ataaggaaag tgatataata cttacactat ttacaagaaa gcttggaaaa gtatctgcaa
     6961 tagcaaaagg agctaaaaaa aataagagct ctttgctttc atcatcacag ttattttctt
     7021 atagcaattt taccttaaaa aaacaaggta atatgtacaa ggttactcaa agtgaaataa
     7081 taaaaagttt ttataatata tcctatgata tagaggcatt ttcttatgct acatatatta
     7141 caaaattagt tgaaaattct atacttgaaa atcagactaa taataggcta tttatattac
     7201 ttgcgcagac tctgtatttg tatacacagg ataatacaga caataggttt ataacagcag
     7261 cattcgagtt aaaattttta gactatatag gatttaaacc tatagtaaat aaatgcatta
     7321 attgtgaatg taaaatttta caaaattctg tgtttaatat ctacgaaggt ggtatactat
     7381 gtagtaagtg tagtaaactt tttgataata atataaaatt agacttaaca actataagtc
     7441 ttatggaata tgtattaaga aatgatattt taacatgtag taaagctaaa gtatcaaaat
     7501 atataacaca tgaacttgag aatattctag acaagtattt gaaagtttat gttgataata
     7561 ttaattttaa atcattacac gtattgcaaa gtgtaaaaaa taataaggga gtggataatg
     7621 atgagtagta atataactat tgaaatggtt gattcagtct ttgaaagagt tccaggagca
     7681 acttatgcag aagcaaaaga agcattagta atgtgcaatg gagatgtaat tgaggcagta
     7741 atatatttag aaaataaaaa aaatacttgt aattgtaaag caaaatctgc taaagaaact
     7801 atggaagaag tatttggcaa agatggagaa aatataaagt ttcaattaaa agagcttctt
     7861 agaaaaagta gtgtagtgcg aataatagtt gaaaaagatg gaaaaactat gatgaacatt
     7921 cctttaacag ttggagttgt tggattagct tttgtaccat tagctacttt agttggatta
     7981 tcagctgctg ttataagtaa atatcgtata aaagttcaaa atgaagaaga tggagaaatt
     8041 gtagatttag gtgaattgaa tgaagaaaaa cttcacgttt tgaagaatat gataactaat
     8101 gcagcaaaag atgtgaagga tgtagtagtt gacaaaaaag aagatgacaa agatgtaact
     8161 gctgacctta tgaaagaaga atctgaaaag aataaagaag acgaagacga aaataaatag
     8221 atataaagtg acttttaaaa aatgaagaga agataattat aagaattatc actaacattg
     8281 acaaatttta tagaatttga tattctaaaa gttgtagaag aactattaaa atagcctttc
     8341 gtgtagacga gaggctttat tgttatagga gggattttta tgaattttca agagatgata
     8401 ctggcgttgc aaaaatactg gtcaaagcaa ggttgtatca tgatgcagcc ttatgatatt
     8461 gaaaaaggtg caggaactat gaatcctaat acttttttaa gatcattagg acctgagcct
     8521 tggcaagtat gctatgtaga gccttcaaga agaccagcag atggaagata tggtgaaaac
     8581 ccaaacagac tatatcaaca tcatcaattc caagttatac taaaaccttc tccagataat
     8641 atacaagaat tatatttgga aagtttaaaa gagattggaa ttgacccaag tgagcatgac
     8701 ataagatttg ttgaagataa ctgggaagct gctacagttg gtgcttgggg acttggatgg
     8761 gaagtttggc tagatggtat ggaaataact caatttacgt attttcaaca agtaggtaat
     8821 attgaatgtg agcttgagac aggagaaatt acatatggat tagaaagatt agctatgtac
     8881 attcaagaag ttgatagtgt ttatgattta aaatggaatg ataaaataac ttatggtgaa
     8941 gtatttaata aagctgagta tgaaaactca atgtacgcat ttgaattatg tgatgcagat
     9001 atgttgttta atctgtttga tatatatgaa aaagaagcat taagacttat ggaaaatggc
     9061 cttgtaatac cttcatatga ctatgtgtta aaatgctctc atgcatttaa tacattggat
     9121 gcaagaggtg caataggagt tagtcaaaga gcttcattta taggtagagt tagaaatatg
     9181 gcaaagactg ttgcagaaac ttttgtaaaa caaagagaag aaatgggatt ccctctttta
     9241 aaggacggtg acaaataatg aaaaattatc ttttatttga aatcggtgtt gaagaaatgc
     9301 catcaagatt tgtaggcagt acactagaac aattaaaaaa caatcttagt aaattatttg
     9361 atgaaaatag aatagctttt gataaaataa atgcgtatgg gacaccaaga agacttactt
     9421 tagtagtcga aggattgagt gatagacaaa atgatttaga agaggaaata aagggtcctg
     9481 ctaagaaaat tgctgttgat gcagaaaata atttaacaaa accagcatta ggatttataa
     9541 aaagcaaagg attaagcgaa tctgacatat attttaaaca agttggaaaa gaagaatata
     9601 tatttgcgac tataaagcaa gatggaaaag aaacttcaga agtactaaaa gacatattac
     9661 cagagactat aaaatctgta gtattcccaa aagctatgcg ttggggagga aaaaatctta
     9721 aatttgcaag acctataaga tggatagttg cattgttaaa tgataaagta ttagagtttg
     9781 atttagaagg aataatatct tctaatgtaa caaaaggtca tagattttta ggtgaaagta
     9841 caatagaagt tgattctata gaagattatt ttgaaaaatt agaaaaaaat tatatagttt
     9901 tagaccaaaa caagagaaaa gaaatgataa gaaaacaatg tatagaagtg gcaaattctt
     9961 taggtggaga agttgaattt gatgaagatt tacttgaaga ggtaacttac ttagttgaat
    10021 atccaactgc tttttatgga gaatttgatg tggattatat aaaactacca aaagaagttg
    10081 ttataactcc tatgaaacaa catcaaagat attttccagt acttaaagaa ggaaaactgt
    10141 tacctaactt tatagctgtt agaaatggtg attcttacag aatagacaat gttaaagctg
    10201 gaaatgaaaa agttttagaa gcaagacttg cagatgcttt atttttctat agagaagata
    10261 ctaagagaaa cttagaaagc tacatagaaa aattaaaaac tgttgtattc caagtaaaac
    10321 taggaactat ttacgacaaa actttaagac ttgaaaaatt aagtgctgat atacttgata
    10381 gtttaggttt agtagatgaa aaggcagata ctataagggc tgctaaatta tctaaggcag
    10441 atttagttac tggaatggta ggagaatttg atgaattaca aggttttatg ggaaaagaat
    10501 acgcaaaagt tggaggagaa aatgatgttg taagtgaagc tatttttgaa cattatcttc
    10561 caagatttgc aggagatata cttcctaaga ctaatgcagg agttgcctta tcaatagctg
    10621 ataagttgga ttcaatagca ggtttctttg ctataggaat acaaccaaca ggttctcaag
    10681 acccatatgc tttaagaaga caagctttag gtatacttag tatacttatg gacagaaaag
    10741 ttgctgtgga tataaaagta cttattgaac atgctttaga caattattct gaattagatt
    10801 ttaataaaga agaagttgtt gagcaaataa tgaatttctt caatgagaga gtaaaaaatc
    10861 tatttaaaga tttaggaata agatatgatg ttgttgatgc tgttttaagt tctgatataa
    10921 atgatgtttc tgatatgcat atgagagctt tagagcttaa taattggcta gaaaaagatg
    10981 aattagtgga gatgcttact gcatttaata gagtttctac tctagctcaa aaagcagaaa
    11041 gtgataaaat tgatgaatct ttattaaaag aagatgctga aattaaactt tacaatgaat
    11101 ttaaacaagt aaaggtaaaa gttaatgaat tattaaagga taagaaatat ggtattgctt
    11161 tagatgaatt tgcatcttta agaccagtag ttgataattt atttgataca gttatggtta
    11221 tggataatga tgaagctatt aagaacaata gacttgcttt acttaaacaa gtatatgata
    11281 ctatgctagg aatttgtgat ttatctaaaa ttgtttataa ataaaattta aactatttat
    11341 aagaaataag gctgtctcaa aatagagata gtcttatttt tcattgttga gatgatgaaa
    11401 atatatttga tttcatagaa aaagagtgtc tcataaacta aaaagttcat tttgagacac
    11461 ccatgctttt tgtaagtaaa tataataaat tatattttat acttatcagt tattttgttt
    11521 aaaatattat catttgaatt tacccattca taaaaattgc ctgatgatgc gtccgatatt
    11581 ttataaagca tttcaggaat atcactagct ttaaaatctc caagtgattt tcccaatcta
    11641 gtcttaccta tttcaaaact gcctccaaca aataattcaa atgcatctgt agatatacca
    11701 tcaacattct tctttttacc acataaacct atgctaccaa cttgatgaac accacatgaa
    11761 tttaaacatc ctgatatata taacttaggt gctttattta aaatttcttt attattttgt
    11821 aatctaaaat aatctatgat ttcatgtaac attttctgac tattttgtat gcccatttga
    11881 caaattggaa cacctatgca agatatactt tgttctaatt gagaattaca actaatagtt
    11941 ttacttattt ctaatattct ttttgcttca tttccattta aatttaaaat atataatccc
    12001 tctgtcattc ctagtctaat cataggattt tcaatattat ctaattcttt aagtaaagta
    12061 cttaaatcct ttgttaacaa aatacctcca actggatgta tatatacagt gtataaacca
    12121 tcctgttttt gaggtattag tctagaatcc tgtatatcta ttttaacccc aggtttagag
    12181 taatctattt cttcaatatt gagttcaaga ctaccatttt ctttttctaa tttatagtat
    12241 tctttaaatt tttctaagaa taatttttca ccaagtgatt cgaccatata tctaacccta
    12301 gctctgtttt tattttcata atttccataa tccataaaga atttaatcat accttcaaca
    12361 caatataagg catctttcgg ttttataagt tcatcaagtt ctaaagcaac tttgggttgt
    12421 ttacctagac ctccacctaa gaaaactctg aaatatggtt catcattttt caaagtagca
    12481 acaaatccta agtcttgtat actacaatga gcagtatctg tgtaacaatt agaataagaa
    12541 acttttaatt ttctaggtaa atggtatgta gtagcatttt tgataaaata tttatcagta
    12601 gcaactgcat atggagtaac atcaaagact tcactagggt caactccaga taatggagat
    12661 aatccgacat ttctaggata attaccacca ccacctctag tgaatatatc attttttatt
    12721 ccttcttcca tgatatcaac aattgaattt atagataaat cgtgtaattg tatagcttgt
    12781 ctagttgtta agtgtatctt atctaagttg tgtttaatag ctatttcata gattttatta
    12841 agttgatact gagataaaac tccagcagat actctaagtc ttatcataaa agattttttg
    12901 tctctttgag cataaacacc atatccacca gaaaatccct tatattgcat tttatttatt
    12961 tctccattta aaaattttga agaatattct ttaaaattag gtagttcatt taacaatatc
    13021 tttttaaaat tatccattat ttcacctcat tgttagtctt gtataaaaat atatctattg
    13081 tataatagat atatatatta ttatctagtc aggataaaaa tataacagaa taattctatt
    13141 aaaagtaaga ataataagga catagtgagt attttgtttt tattttacaa attaagtata
    13201 atatttttgt tttataaaat ataattaaga taaatagata attttgtaat tttaatatat
    13261 aatatttagt agagtgtggg agtagcactt aatatttgaa ggaggagttt ttgtatatgg
    13321 gagaaataat aaatgcatta gatagagccc tagacataat tttgctgtta tatcatgaaa
    13381 agagagaaat gggaataact gaaataagca aggctatggg agtatataaa agtactgtac
    13441 atagaacttt ggttactctt gaaaacaaag gttttgttat acaaaatgca gagaatggta
    13501 agtattggct aggaataaat ttatatgcaa taggtatggt tgtgggagaa aaaatgtcat
    13561 tgactgaaat agtaaaacca tatacgaaaa aattaaatca agaatttaac gaagtagtta
    13621 atgtatctat attagaagaa agagctcaag attctccaag aagtatcata atacataaag
    13681 agtatggttc aaatcaatta ctttcagtaa atccatccgt tggttcaagt agtgaatgtt
    13741 attgttcagc agttggtaaa tgtcttatgg catttaatga ttctatagat tttgaaaaat
    13801 atagaaaaac accaatacac aagtatacag aacatacaat tgataattgg gatgatatga
    13861 tgcttttttt agcaaaaata aaagaacaag gatatgctat agatgatgaa gaactagaac
    13921 atggactaac ttgtattgga gcacctatct tagataaaaa taataaagct attgcagcca
    13981 taagtttgtc aggtccaact ataagaatga gagaagggga ctttgagtat aagattaaaa
    14041 gagttataga aactgcaaaa agtatatctg aactttttag atgattttta atctcgataa
    14101 taaaaatagg ctatatttaa attgttattt gtaaatatca gtttaaatgt agcctatttt
    14161 tctttaatct aaaattaaat attcttaaaa tttaaaatgt tattgaaata tataagcgaa
    14221 tgtgttatct tttaattaag caataaaaat agaacaaggt tctatactat agaacaaaat
    14281 tgatgcatat atagtattta tacaaaatat aaatattata tatttaaaat taagtaaaaa
    14341 tattacataa aggggagatt tgagaaatgg aaaaagtcaa gttgagtgtt gaaagatgta
    14401 agggatgcta tctttgtatt gaagcttgta aaaaagatgc aataagtctt tctgactaca
    14461 caaataaaaa aggctttgta actattaaag tcgatgaaga aaaatgtatt caatgtggcg
    14521 cttgttatac aatgtgccct gatttagtat ttgagatatt ataaattatt tttattagag
    14581 gggagaaaag gtatgcaaag tttattaatt aaaggaaatg aagctttagc agaaggtgct
    14641 atacgtggtg gctgcagatt ttatgcaggt tatccaataa cgccacaatc agaaatatta
    14701 gaaatgatgg catggagaca aaaagaagtt ggaggagtat ttatccaagg tgaatcagaa
    14761 atagccagtg taaatatggt tatgggagca agtgcacttg gagcaagagc tatgacttct
    14821 tcatctggtc caggattttc attaaaacaa gaaggtattg catattgggt atcagctggt
    14881 ttgccagcag ttgtagcatg tgtgcaaaga tatggtatag gtgatggatt tataggagct
    14941 ggtcaagata attattggca agcagtaaaa ggcggtggaa atggagatta tcatttaatc
    15001 gtgttagcac ctaatagtgt tcaagaaaat atggatatgg catatgaatc ttttgaattg
    15061 gctgaaaaat atatgcatcc agtacttata ctttctgatg ctacaatagg tcaaatggta
    15121 gaaccttgta tagcaccacc aatgaaagaa tacgatatgg ataaagctcc atgggctgca
    15181 aaaggaacac ctgtaggaga aaaaaataaa gtaataactg atataacata ttttgaagac
    15241 caagatacat ggcaaaaagg gtatatagaa aaaatgagaa aagttcaaga agatgaacaa
    15301 agatgggaag agatagaaat agaagatgct gaagtggtat ttgtggcata cggtatttca
    15361 tcaagaatag cagaaggtgc agttagagat gctagagcaa aaggaatgaa attaggtcta
    15421 ataagaccaa aaacattgtg gccttaccca agaaaagcat ttaagaaatt aagtgattca
    15481 ttgaagggac ttgtaacagt tgaaatgaac atgatggcac agatgaagga agatgtaatt
    15541 atagcatgtg ataatagatt tccagtatat tcagtagcaa cagctcaata tatggcagaa
    15601 gtaaatcaat taatagattt tgctcaacag attatagatg gaaaagctaa gcaagaggag
    15661 gtattttaat tatggctaaa aaagcaccag caataatgat gtgtgaaaat atgttctgtg
    15721 gaggctgtgg acactctata gtcgatagaa ttattggaga agtattaaca gaaatggata
    15781 tatgtcaaga tacaattata tgttcagata taggttgtaa tcatatgttt caatttagta
    15841 tgaatgtaga tgttgtcgtt ccacctcatg gtaaaatggg agcagcaatg gcagctatga
    15901 aaagagttag accagataaa tatatattta caatagctgg agatggtgga gcttatgcaa
    15961 tagggcttgg agaaactatg tcagctgcta ctagaaatga taatgtaata tttcttgtta
    16021 tgaacaataa tatgtttgca atgacaggag ggcaaatggc tccaactact atagaaggtc
    16081 aatatacagc ttctactcca gcgggtagaa attacgcaga tcatggttca acatttgatg
    16141 ttgtaaaagt tatgggaaat ttagatatag catatctagc aagaggaaca ataactaacc
    16201 ctaaagaaat aaatacaaca aaaagataca tcaaaaaagc tctagaaaaa caaaaagaaa
    16261 agaaaggttt ttgcttaata gaagtgcttg taccatgtcc aacaaactgg ggattaaagc
    16321 cagtagatgc tatgaagaga attgaaaatg aacttgttcc aaagtatcca cttggagaat
    16381 ttattgatta gggaggaaat aatatgttga atgaaattat atgtgctggt tttggaggac
    16441 agggagttct tacagcagga aaaattataa tgtatgttgc atataaggca gaaaagaaaa
    16501 ttacatggtt cccatcttat ggaaatgaga tgaggggagg aagtgcaaat tgcaatgttg
    16561 ttgtaagtga tgaaaaaatt gcaaatcctt gtgtaaaaaa tccagatata ctattgggaa
    16621 tgaacagcgt atcagtagat aagtatgaaa gtttaatgaa aaaaaatggt tatatgtttg
    16681 tcaatgaatc actagtaagt aaggatagaa agtacagaga agacattaat gttataaaag
    16741 tacctgttac tgaaatagca caaaatttag gcaatgaaaa agctgctaac atttgcatgt
    16801 taggtgctat ggtaaagagt ttggatttat ttacaaaaga agaatttgaa agtggtatgt
    16861 gtgaatactt tgaaaatcaa ggcaaaggta aattcaattc taaaaatgta gaagcatttg
    16921 aagctggata cgactacgaa tgaatatttt aaatatttaa aataattatt gaaatactta
    16981 tttttatatg ttagtattta attaataaaa tatagaacgt cgttctggac aatgggacga
    17041 agtctttagg tcggaataca agaacggcgt tctgtataat agaactaggg ggtgttaaat
    17101 tgaaagtttt agtaataaat cctggtggta catccacaaa aatttctgtt ttccaagatg
    17161 aaaatgaaat tttgaaaaaa aatataaccc atacaagaga agatttaaaa aatttttcta
    17221 aagtatttga tgaatatgat tatagaaaac aactgatagt agatatttta agtagtgaga
    17281 atcatagtat aaattctttt gacgcagtag ttggtcgtgg tggacttatg aaggctataa
    17341 aaggtggtac ctatactgtg agtgaagaaa tgattgagga tatgagaaat gaaataaatg
    17401 gggaacatgc atcaaattta ggtgcattat tagctaaaac tatagctgat gaaataggag
    17461 tacaatcatt tgtagtagac ccagtttctg tagatgaatt tgatgatgta tcgaggatta
    17521 caggaatatc agacatagaa aaagcaagtt ggttacatgc attaaaccat aaagctgttt
    17581 caagaaaaat tgcacaaaaa ctagggggaa aatacgagga ttataacttt attgtagctc
    17641 atttaggttc aggaatatcg attgtagcac ataaacaagg caaaatgatt gatggaagtg
    17701 gtggtaggtc tgatggtcca ttttctccag aaaggagtgg tggattactt gcatatcctc
    17761 ttatagaatt gtgttactcc ggaaaatata ctcatgatga aatggtcgca aaagtaagtt
    17821 cgattggggg aatgtacgat tatttaggaa caaaggacat gatagaaatt gaaaacagaa
    17881 tcaaggaggg tgatgaaaag gcaaaactta taatggatgc atttatttat caagtagcaa
    17941 aagaaatttc tatgtatgga gcttcacttt cagggaatgt agatagagtt atcttgacag
    18001 gagggatatc tcactcagaa atggtagtca aaggagtagc agaaaaagtt tcatatctag
    18061 ctccaataga gatagtacca ggagagatgg aaatggaggc acttgctcta ggggcactta
    18121 gagtacttaa tggagaagaa atagcaaaaa cttattaata tttaggagaa agagtatgtt
    18181 aaaagatttt aataatttat tagaagaggc taaaaagctt gaacctgtta ttgtaagtgt
    18241 tgctgttgca gaagacaaag aagtattaaa ggctgtgaaa tcagcaacag atttaaacat
    18301 tataaagcca attcttgtag gagacaagga taaaatagaa agtatatcaa gagaaattgg
    18361 attaaaaggt catgaaataa taggttgtca aaatcccgat gaatcagtta aaaaagctgt
    18421 agagatagtt aaaaataagg aagctaaagt tcttatgaag ggattagtaa atacaagtgt
    18481 ctatatgaga gctatattaa ataaagaaac gggtcttaga acaggaagat tattaagttt
    18541 gcttgcagta tatgaattac ctcaatatca taaacttctt tattgtactg atagtggaat
    18601 aaatgtatcg ccaaacttaa atcaaaaaaa agatataatg acaaatgcac ttcttactat
    18661 gaaaagtata ggcatggaaa atcctaaaac tgcgatactt actgcaaatg aaatggtaga
    18721 ccctaagata caatctacag tggatgccaa cctacttgta gagatggaaa aatcgggaga
    18781 aataccaaag tgtatagtgg aaggtccaat agcatttgat gtagcatttg atgcacatgc
    18841 agcagaacat aaaggtatta aaagtaatat tgctggtgat gttgatttat tagtatttcc
    18901 aaatatagaa gcagggaata tattaggaaa gtcatggctt agatttaata aagctaagtg
    18961 ggcaggtata gttttaggag caacaaatcc gataatttta ggttcacgtt ctgacacagc
    19021 tgaaataaaa ataaattcaa tagcattagc gtgcctagca tcacaataag aaattattat
    19081 taaggaggaa aaagtattat gaaatatgca aaaagaatgg atatggtaaa agcttcagct
    19141 ataagagatt ctcaaaagaa aatagctcag aaggtagcaa gtggtggaac tgttatatct
    19201 tttgctgcag ggcttcctga cccaaattta tttccaatag aagagataag tgaaataact
    19261 caaaaagtac ttaatgaaag gggaaaattt gccctagcat atggacctac taaaggtgaa
    19321 gatgagttgt tagatctatt agtaaaaaga atgaaagaag aagaaaatat tgatacaaaa
    19381 gcagaaaata ttataataac tactgggtca caacaaggga tagcattaag tgctatgatt
    19441 ttattagaaa agggagatgt agtagttaca gaaaatccaa gttacttagg tgcaataaat
    19501 gcatttagac cttatgaatg tgatttttta ggagtagata cagatgaaga aggaatggta
    19561 acagatgatt tagacaaatt attatctgaa aattctaaca taaaggtgat atatgtaata
    19621 ccaacatttc aaaaccctac aggaaaaaca tggtctttac aaagaagaaa agattttatg
    19681 gaagtcgtga ataaatatga tgtcatagta atagaggata atccttatgg agaaataaga
    19741 tttacaccag aacctctacc tacacttaaa tctcttgata ctaaagataa agtattgtac
    19801 cttgggtcat tttcaaaggt attatgtcca ggatttagag ttgcttggat gtgtggtaat
    19861 gtagacatca tagataaggc agaactttta aaacaaggta tagatttaca gtcaaatcaa
    19921 ttttcgcaag tacaagtaat agaattttta aagaaataca acttaaatga acatatagaa
    19981 aaaataagag cagagtataa gaaaagatgt ctgctaatgt tagatgctat gaagaaacat
    20041 ttcccagagg aagctaaata tacagaacca gatggaggaa tgtttatatg ggttgaactt
    20101 cctgaaagta taaatgtaga tgatttatta gataaagcca ttgatgctgg agttgcttat
    20161 gtttctggag agtccttctt tgcaaataat ggtccaaaaa atactatgag acttaactat
    20221 acaacaatgt cagaagaaca aatcgttaaa ggtgttgaga tacttgcaga agttataaaa
    20281 aaagaactca attatgcttg tgaataaata taaaaagtga gggtttaaat atgggagaaa
    20341 aagaaaacgg tataaagttt ggaaagttaa tgtttcaact tgttattata gcaatatctt
    20401 gggaattagt ttatattata ccatttatcc aatatacatt atatgatcca atcctaaaag
    20461 cattagaatg tacaaacact caactaggtt ttctacttac aatttatggg ttaggtaata
    20521 tatttggagc tcctatagga ggctggttag cagatagatt tgattataga aagataattt
    20581 taggttctgt atttcttaat ggagtagtat catttttatt tgcatttaat atgaactatc
    20641 catttgcagt tgctacttgg gtaggttgtg caatcacatc attagttatg aactatcctt
    20701 ctatggtaaa aatacttaga gttataggaa aagataatca aggtaaggtt tatggattta
    20761 atgaagctat ggttggagtt tctggagtag taatgggagc aatattctta tatatttata
    20821 catgctttgc aactcctaca ttgggtatga gatgggtaat gatatcttta ggaatattat
    20881 caatagcaat gtgtccagta ttatggtttg taattaaaga tgtagatatt aaagaagaaa
    20941 gagaagaagc aggtgaaaaa atgtctgctg gtgattttat aacagtacta aaatcaccaa
    21001 atacttggct agtaggaata agtatattct gtgtatattc attcacagtt acaatgtctt
    21061 attttacacc atatataaca tctgtacttg gaggttctgt agcattatct ggtgcattag
    21121 caataataag acagcatggt ctaaaattat ttggagcacc atttggagga tattgtgcag
    21181 acaaattaaa atctcctaca aaagtacttt taccaatata tgtatttggt atagcagtaa
    21241 tagttttatt cctagtatta cctgcatcaa ctccaatgac tatattcata gctttaacat
    21301 ttgtagtagg tatattaggt tacatgggaa aaggtatata ttatgcagta caagatgaag
    21361 taaaagttcc agttaaatat tcagcaacta ctataggtat agctgcagct ttaggatttt
    21421 caccagatgt attccaattt gcattaatag gacactggat agatacttat ggcaataaag
    21481 gttacacata tacatttata ttccaaatag caattctagt tattggaata ctatcttgtt
    21541 tatatatatt aaaggttaaa aagagaagag caagtaaatt agaagcttaa atattcaatt
    21601 tggtaattaa gtatatataa atacaatact tattttgatg aatgtgattg tataaataac
    21661 ataaaaaata tcaatagatt aagttttata ataatgttta gagaataaag tataaaaatc
    21721 aaaatgttat caagttttct aggttttaac ataaagttac ccctgaaggt gtaaaattga
    21781 gtggcaatta ttatctattt tcttacctac ataaacctat ttaatttaaa ttctaagaga
    21841 ttttcaaaat agatggtagt tgtactcaat tttaaacctc ataataaata cga
//
LOCUS       DAYAOY010000068        21835 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYAOY010000068 DAYAOY010000000
VERSION     DAYAOY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.068"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            220..1671
                     /locus_tag="ACGI6B_002630"
     CDS             220..1671
                     /locus_tag="ACGI6B_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9752065.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1890..2351)
                     /gene="ribE"
                     /locus_tag="ACGI6B_002631"
     CDS             complement(1890..2351)
                     /gene="ribE"
                     /locus_tag="ACGI6B_002631"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFG9752066.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2456..3649)
                     /locus_tag="ACGI6B_002632"
     CDS             complement(2456..3649)
                     /locus_tag="ACGI6B_002632"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFG9752067.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3672..4337)
                     /locus_tag="ACGI6B_002633"
     CDS             complement(3672..4337)
                     /locus_tag="ACGI6B_002633"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFG9752068.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(4371..5483)
                     /gene="ribD"
                     /locus_tag="ACGI6B_002634"
     CDS             complement(4371..5483)
                     /gene="ribD"
                     /locus_tag="ACGI6B_002634"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFG9752069.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5615..5736)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5925..8408
                     /gene="recQ"
                     /locus_tag="ACGI6B_002635"
     CDS             5925..8408
                     /gene="recQ"
                     /locus_tag="ACGI6B_002635"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFG9752070.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8497..8608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8750..10051
                     /gene="thiC"
                     /locus_tag="ACGI6B_002636"
     CDS             8750..10051
                     /gene="thiC"
                     /locus_tag="ACGI6B_002636"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFG9752071.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            10105..10299
                     /gene="thiS"
                     /locus_tag="ACGI6B_002637"
     CDS             10105..10299
                     /gene="thiS"
                     /locus_tag="ACGI6B_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFG9752072.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10292..11095
                     /gene="thiF"
                     /locus_tag="ACGI6B_002638"
     CDS             10292..11095
                     /gene="thiF"
                     /locus_tag="ACGI6B_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HFG9752073.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11158..11928
                     /locus_tag="ACGI6B_002639"
     CDS             11158..11928
                     /locus_tag="ACGI6B_002639"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFG9752074.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11945..13048
                     /gene="thiH"
                     /locus_tag="ACGI6B_002640"
     CDS             11945..13048
                     /gene="thiH"
                     /locus_tag="ACGI6B_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFG9752075.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            13049..13738
                     /locus_tag="ACGI6B_002641"
     CDS             13049..13738
                     /locus_tag="ACGI6B_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFG9752076.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13926..15320
                     /locus_tag="ACGI6B_002642"
     CDS             13926..15320
                     /locus_tag="ACGI6B_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFG9752077.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15837..17204
                     /locus_tag="ACGI6B_002643"
     CDS             15837..17204
                     /locus_tag="ACGI6B_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFG9752078.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17229..17837
                     /locus_tag="ACGI6B_002644"
     CDS             17229..17837
                     /locus_tag="ACGI6B_002644"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFG9752079.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            17885..18256
                     /locus_tag="ACGI6B_002645"
     CDS             17885..18256
                     /locus_tag="ACGI6B_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFG9752080.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18435..18773
                     /locus_tag="ACGI6B_002646"
     CDS             18435..18773
                     /locus_tag="ACGI6B_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFG9752081.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19239..19742
                     /locus_tag="ACGI6B_002647"
     CDS             19239..19742
                     /locus_tag="ACGI6B_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HFG9752082.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19789..20748
                     /gene="moaA"
                     /locus_tag="ACGI6B_002648"
     CDS             19789..20748
                     /gene="moaA"
                     /locus_tag="ACGI6B_002648"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFG9752083.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20752..21222
                     /gene="moaC"
                     /locus_tag="ACGI6B_002649"
     CDS             20752..21222
                     /gene="moaC"
                     /locus_tag="ACGI6B_002649"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFG9752084.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21258..21689
                     /locus_tag="ACGI6B_002650"
     CDS             21258..21689
                     /locus_tag="ACGI6B_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HFG9752085.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8431 a         2349 c         3762 g         7293 t
ORIGIN      
        1 ataaaatatt aaatatatct atattataaa aacaataaaa aaaggtttga tttgaatata
       61 aaactgcaat atattaaaaa aattattcat tttaaacctt gtaatttgat tttataaata
      121 ttataattaa attaatgaat ttttaacaag gaattattca tttttttgat tttaagattt
      181 attgagaaaa caaaactaaa taggggggta attaatataa tggatttttt caatggtatt
      241 ttaacgagta ttaacaatgc agtagtatat gtaaacaatt acttatggtc gtatatctta
      301 attataatgt tagttgtaat tggtatttac tttactatta aaacaaattt tgtacaattc
      361 agatatttta tagaaatgtt tagacttctg ggtgatggta cagcgaataa aaatgcaaaa
      421 aaggaaggaa aaatctcttc atttcaagca ttttgtataa gtacatcttc aagagttgga
      481 actggaaata tagctggaat agcaattgca gtggtagcag gaggacctgg tgctgtattt
      541 tggatgtggc ttatagcatt aattggttct gcatcaagct ttgttgagag tacattagct
      601 cagatttaca aagtgaaaga tggtcaagct tttagaggtg gaccagcgta ttatatggaa
      661 caaggtctaa ataaaaaatg gatgggtatt ttattctcaa tacttataac tatatgttat
      721 ggttttgtat ttaatgcagt tcaagcaaat actgtttcac ttgcatttaa taatgcattt
      781 ggaataagta aaatggctat gggaataggt cttggaattt taactgcaat agttattttt
      841 ggtggagtgc atagagttgc aaaagtttct gaaataatag ttcctgtatt tgcaggatta
      901 tatatattag ttgctttggc tatagttgta atgaatataa cagagatacc aagtgttata
      961 gcattgatag ttgaaagtgc atttgatttt aaaggaatgg cgattggtac atttatggga
     1021 gttgtaatga ctggagtgaa gagaggtcta ttttctaatg aagcaggtat gggtagtgct
     1081 cctaatgctg ctgcaacagc acatgtaact catcctgtaa aacaaggttt aattcaatct
     1141 ttaggtgttt ttacagatac tataataata tgtagttgta cagcattcat ggtattatta
     1201 tattcaggtt atagtagtag tggagcaact ggaattgaat tagctcaaga agctttaact
     1261 catcatatag gaccagttgg aaatatattt atagcagttt gtatattctt gtttgcattt
     1321 agttctatag ttggtaatta ctattatggt gaatcaaata tggagtttat gagtggaagt
     1381 aagactaaac ttaatgtatt tagagttttt gttgtaggta tggttttatt tggttcactt
     1441 actcaagtgg atgtagtttg gaatcttgca gacttattta tggcattgat ggcgataata
     1501 aacttaatag caatagcact tttaggaaaa tatgcattta ttgctttaaa agattataca
     1561 agtcaaaaaa aatcaggaat aaaagaccct gtgtttattg cagatgaaat agaaggccta
     1621 gaaaaggtat cagagtggca tagggaatta tctgaggaac atttaggata aaaaatagta
     1681 taaaattata aaaccatttt gaaagactag agtatgtata tactttatat gaatataata
     1741 ttgaattaat ttatattact ggaattttta attccagtaa tattttttta ttatataatt
     1801 tagctttatt aaaagttttt attattaaaa atagcttatt tatcgtaaag tgaatgatat
     1861 tgggttttaa ttattatgaa tgttagtatt taatttaaag acttaaataa atttgccatt
     1921 tctagcgcat tcatagcaca atcataacct ttatttccag cttttgtacc tgctctttca
     1981 atagcttgtt caatattttc tgtagttacg attccaaata taacaggtaa ttcactgtct
     2041 aaagatactt tggctatccc tttagagact tcactacata cataatcata atgagaagta
     2101 gcccctctta taacacagcc taaacagatt atagcatcat acttaccact tttagccatt
     2161 ttttgagcaa caagaggaat ctcaaaagct ccaggtaccc aaactatttc tatattttct
     2221 ggagaaacgt catgtcttaa aagacagtct tcagctcctg ataaaagttt tgatgttata
     2281 aattcattaa aacgagaatt tattatacct atttttaaat cttttccaat aagttttcct
     2341 tcatatatca tttttacttc ctccaatttt atttatgtta tagatttata tttataaata
     2401 tctttaattt aattataaaa aactacttag tagaaaaata tcatcatata atcatttata
     2461 taatatctaa aagatgtccc attttatctt ttttagtttt taaatatagc ttattaaagt
     2521 cattaatttc ttcattaata gggattctat tttcgatttt tattccatat ttctctaatt
     2581 gatttatttt atctgggtta ttacttaaaa gatttatact ttttatgttt aaatttttta
     2641 atatttgagc agccatatag aaatctctca tatcttcttc aaatccaagc attaaatttg
     2701 cttcaacagt atcgtaacct tcttcttgaa gtttataagc cttaatttta tttaaaagac
     2761 caatacctct accttcttgc ttcatataaa ttaaaactcc acttccattt tccacaattt
     2821 tcttcataga agattctagc tgtaatccac aatcacattt tagagagtgg aacacatctc
     2881 ctgttaaaca ttcagaatga agtctaacta gagtattttc tatatttata tttccataag
     2941 taagggcaat gtgttcttca tttgaataag tatctctata tcctatgatt tcaaaatttc
     3001 catattttgt cggaaggaaa gcactagata ctttttctat agtagtttca tttattttgc
     3061 ggtattctat aaggtctttt atagtgatga tttttaaatt atgcttttct ttgaaaatca
     3121 ttaaatcatc aactcttgcc attgtaccat catctttcat tatttcacag ataagaccaa
     3181 tctctttaaa acctgcaagt ctagcaaggt ctactgaggc ttctgtatgt ccatccctga
     3241 caagaacccc accagttttt gctattaaag gaaagatgtg accaggcgtt ttgaaatctt
     3301 tacttgtaga attttcatca cagaataatt ttacagtttc agctctttca tatgcagata
     3361 taccagttag agtatctgct ccatctatag taacagtaaa ggctgtttga aatgtatctg
     3421 tattttctct aaccataaga ggtatattaa gacttttaaa ttttttttca gtggcaggta
     3481 gacaaattag acctctacca tatgtagcca taaaatttat tgattcagga gtagccattt
     3541 cagctgccat caataaatct ccttcattct ctctactttc atcatcaacc acgattacca
     3601 ttttcccatt ttttatatct tcaatagctt cttcaatagt attaaacatg taattcactc
     3661 ctttattatt tttaaaaacc atttttaaat aaaaaatctt tatctatatt tgatttatgg
     3721 ttattactat tagaatttaa ttccttgtag ttattagcca taaaattatt aacatacttt
     3781 ccaattacat cgctttctaa attaacgaaa tctccaacat tctttgtcaa gagaatagtg
     3841 ttaattttag tatgaggtat aatagaaacc ttaaatattt catcatctaa atatgatata
     3901 gttaaactta caccatcaat agcaacagaa cctttgagta tcattgagga taaaagtttt
     3961 ttatcaggtc gtatacttac taaaactgca ttttcatctt tttcaaattt gcagatagtt
     4021 cctttaccat caacatgacc tgttactata tgaccaccaa atcttgaaga aagactcata
     4081 gctctttcaa gatttacctt gtcatttgca tttaataaac ctaaatttgt ggaacgaatt
     4141 gtctccatca taacatcggc tgtaaattca ttttttccga ggtttgaaac agttagacaa
     4201 actccattta cggctatact atctccaaga tttgtgccat ctaatatttt atttgatagt
     4261 atggttactt ttccactctt accattagca gtaatttttc taagtattcc aacttcttct
     4321 actatgccag taaacatcct aacctcctat atgaatgtat taacaaaaat ttaatttaaa
     4381 acatctgatt ctataagaat atcgccatct atcaaagtta cattattgat tgttaactgt
     4441 gttgaatctg acaaatgatt tattccttct ccagaaacga atgtaggtgc atctttacca
     4501 cctataatct tgggtgatag aaataatttg accttatcaa ctaacttatt tctaaagaga
     4561 ctgtcattga gagtagcacc accttcaact agaacagaat caatgtttaa ctctcctaat
     4621 ttatacatta caaactccaa atctactaag ttgttttttg aaggagaaat tattgtttca
     4681 acattatttt cttttaacat agataatttt atactttctt tgcaacatgt gaaaactata
     4741 gttttactgg ttttatcttt aaccaaatta ctattaagtg gcaactttag gtgtgtatct
     4801 attactattc ttgtaggatg agacactttt tcttgattga gcctacaagt tagtaaagga
     4861 ttatcgttca aaactgtatt tatgccaacc attatacttt ggtatttatt tctgtattta
     4921 tgagttagat accttgatga ttcattagat atccattttg attctagtga ttttgtagca
     4981 attttaccat ctagacttac tgctgatttt acaatacaaa gaggtctttt gttttttata
     5041 tgataaaaaa atgcctcatt taaatctctt gcttcatctt ctaatattcc cacagtaaca
     5101 tcaatattgt tatcttttaa tttttttact ccattaccac aaacaagtgg atttgggtct
     5161 aatgtagata ttacaactct cttaatctta ttttgtataa ttttatctac acaaggaggc
     5221 gttttcccat aatgagaaca tggttctaag tttacataaa gagtagagtc ttttagactt
     5281 tgtttagcac tgtttatagc atttacttca gcatgattag agccaaattt ctcatgatat
     5341 cctttgccta ttatattact agctttaaca attacacacc caactaatgg atttggattt
     5401 acaaagcctt ctccattttt agccaattca atagcttttt tcatataata aatatctttt
     5461 tctttttgat tcacaaaatt cacaatatgt acacctcctg tttaattgga taatttttaa
     5521 acaaaaaacc ctgagaatta actcagggtt tagaataatc tacaataaat gtttaataaa
     5581 tgtagaacaa aattgctcta caaaaacact cattaaatat cttcttccat ccagacttta
     5641 ctgtcggctt tggaatttaa ccaaatcaac caagttttac cttggctcgc gggctatacc
     5701 gccgattggg aatttcaccc taccccgaag attaagtatt aaatttatat ttagaatgta
     5761 tcataaatta ttttactttg caaactattt ttttaaaaaa tatatgttaa tatataatat
     5821 agaatgtcta taaactttga aataccacga ttgtgaatat aaaaataaaa atttgaacat
     5881 aatcaggaaa gaagtctaat taatttaaat taggagagta atatatgaat aaaaaacttt
     5941 tagaaatact atcaaaatac tatggatata caagttttag aaaaggtcaa gaatctataa
     6001 taaactctat cttaagcgaa aaagatgtgt tagctattat gccaacagga gggggaaaat
     6061 caatatgtta tcaaattcca gctttgattt tagatggaat gacaatagta atttctccac
     6121 ttatatcact tatgaaagac caagtagatg ccttaaagac tatgggaatt agtgctgctt
     6181 ttataaatag ttcattaagt agcaaagaat ttaatgaaat attaaataat attagaaaaa
     6241 ataattataa aatactctat attgctccag agagattaga tgcacaagag tttattgagc
     6301 taataaataa taacaatgta agtcaagttg cgatagatga agcacattgt gtatctcaat
     6361 gggggcatga ttttaggttg agttatagaa ggatttctga ttttataaaa aatttgccta
     6421 aaagacctat agttacagca tttactgcaa cagcttcaga agaggtaaga actgatataa
     6481 taaatttact gtgtttagaa aatccagatt attatataac tggatttgat agagagaatt
     6541 tatcaataaa tatagtaaaa tcatctagta aaaataagta tattttagat tatattcaaa
     6601 atcataaaaa tgagtctgga attatatatg ttgcaactag aaaggaagtt gaaaatatat
     6661 ataatggttt atctaagaga aatatatctg tgtcaaaata tcatgcaggt ttatctaaaa
     6721 atgaaagaag taaaaatcaa gaagatttta taaatgataa tgtggatata atggtagcaa
     6781 caaatgcatt tggaatgggt attgataaac ctaatataag atgggttata cattacaata
     6841 tgcctcaaag tatagaaaac tactatcaag aaataggtag agctggtagg gatggagaaa
     6901 aaagtgagtg tgtactttta ttttctccag gagatgtaca tatacaaaaa tatttaattg
     6961 atattggagt tgaaaatcct gaaagaaaat tatttcagca taagaagctt caatacatga
     7021 ttgacttagt ttacagtaat tcatgctata gaaagacaat attgagttat tttggagaaa
     7081 attatataga caattgcaat aattgtagca attgtttagt agagggtgaa atagtagata
     7141 aaactataga tgcacaaaaa gtcatttcat gtgtagctag aatgaaaaga agttatggtg
     7201 ttactactat tgtagatgtg cttagaggtt ctaagaataa aaagatttta cagcttggat
     7261 taaatacttt gtctacatat aacattatga gagattattc tagtgaagac ttaaagaatt
     7321 ttgtaaatac attggtttct catggttttt tagatttagt tgagactcta ggcaatggaa
     7381 atagaggtag ttttcctact ataagattaa atgagatgtc tatgaaagtt ttaaaaggtg
     7441 aagtgaaagt agagtttaaa gaaattgtta tgacaaaatc attggaagta gaggatgagt
     7501 tatataatgc tttaagagaa cttagacatt caattgcaag tgaagaaagg atagctccat
     7561 atatggtatt tggagatggt acacttagag ccatgtctag tagttatcca attaataaag
     7621 aagaaatgct agatatctct ggagtaggag aaattaaata tcaaaaatat ggtagagatt
     7681 ttgaagcagt aattaaagaa tatgtcgaaa aaaataatat aaataaaaaa atagatgaaa
     7741 aaaatgtcga ttcaaagaac gttaatagtg atttcttaga ggttaataca gataaagttt
     7801 tatatgaaaa actgttagtc attagagaag aatatgcaac aaaggaaaaa ttacctgcat
     7861 atatgatttt agccaataag gctttgaaag aaataagtgg aagatatcca cttgatgaag
     7921 aacaattaaa agatatttct ggcataggtg gagtaaaatt tgaaaaatat ggagctagta
     7981 ttttggcaga ggttaataaa tatgtaaaag aaaataatat taatgttatt tgggagaaaa
     8041 agggcaaaag gaagttaata ttagatggag aaagtagaaa aaataatgaa atagcattag
     8101 atttgctaaa tcaaggaaaa gatatacaaa gtatcagtca agaaatagaa gtttcactgt
     8161 caaccattat tggatatgtg catgactatg taaaggatgg acatagtata tcatttgaat
     8221 taggactaga aaatttttat aatgaagata aaaagaagat gattttagaa gtttgcgaaa
     8281 aagttggcta tgataattta aataacataa aaagaaaact tccagattca ataaagtatg
     8341 aaaatataag agctgtgata cttgactctt atcttgaaaa cttaaaaaat aatattatag
     8401 cagagtaatt tttaacttaa taaaaaataa aatttgtatt attgtgttga actaataatt
     8461 ttgctgtgtt atactgaatt tgtaaaaata atgaaaaatt agagctaggg gcgctagaat
     8521 tggctagctg agagataaag ccgtaactta caaaccctaa taacctgatc aagataatgc
     8581 ttgcgaaggg aagcttttta atataatttt attatataaa taggcaaatt attcaatgtt
     8641 aggagaaatt tgtctaagtg tatatacgat tataagagtg ctttccaaac gaggaaaagc
     8701 acttttttta ttttcaaaaa ctataacttt aatcttggag gattgtaata tgaactatac
     8761 aacacaaatg gatgctgcta gaaaaggcat aataactaaa gaaatggaaa tcgtttctca
     8821 aaaagaacag gtagatgtaa atgaattaag agaattaata gcaaatggac aagttgttat
     8881 accagctaat aaaaatcata aatctttaag tgcagaaggt gtaggtaaaa atttaagaac
     8941 aaaaataaat gtaaatctag gaatttcaag agattgtaag gatatagaaa aagaacttga
     9001 aaaagttaga gtagcaatag acatgaaagc tgaagctata atggatttaa gtaattatgg
     9061 caaaactaga gaatttagag aaaaggttgt tgaaatgtca cctgcaatga taggttcagt
     9121 acctatgtat gatgcagtag gatatttgga aaaagaatta aaagatataa cagaagaaga
     9181 gtttttaaat gtaataagac aacatgctat tgatggagta gactttatta ctatacatgc
     9241 aggtcttaca agaagtgttt gccaaaaaat aaaaaatcat gagagattga ctcatatagt
     9301 atctcgtgga ggttctttat tgtttgcatg gatggaatta aataataagg aaaatcctat
     9361 ttatactaat tttgataaaa tattggatat atgtgaagaa tatgatgtta cattgagttt
     9421 aggagatgct tgtagaccag gttgtataaa agattctact gatggtgttc aaattcaaga
     9481 acttgtagta ttaggtgaac ttactaaaag agcatgggaa agaaatgtac aagtaatgat
     9541 agaaggtcct ggacacatgg ctattgatga aatagaagca aatgtagtat tagaaaagag
     9601 attatgccat ggagcacctt tttatgttct tggaccattg gttacagata tagcaccagg
     9661 ttatgaccat ataacaagtg caataggagg agctttagca tgtgccaaag gagtagattt
     9721 tttatgttat gtaactccag cagaacattt aagattgcct aatttagatg atatgaaaga
     9781 gggtataata gctgcaaaga tagctgctca tgcaggagat atagctaaaa atgttaaagg
     9841 tgctcgtgaa tgggataata agatgagtaa agctagagct gatttagact ggtgtgagat
     9901 gtttagactt gcaatagacc ctgaaaaagc taagagatac agagatgaat caactcctac
     9961 tcatgaggat agttgtacta tgtgtggaaa aatgtgctca atgagaacag ttaagaaaat
    10021 attaaacaat gaggaactta atctaatata atttttggct agtatgactg gaataaaaat
    10081 aagctgaaaa gaggaggtga tttagtgaag gttaatggga aggaaattga atttgaaaaa
    10141 gatttgactg ttatagattt attaaataaa tataatttaa aaagtgatag agttgtagtt
    10201 gaagtgaatt tagagataat tgaagaatct aactacaata catatgtatt aaaagacgag
    10261 gacattgtag agcttattag ctttattgga ggtggttaaa ataaaaatat ttgtaaatga
    10321 attattaaca gatgttgagg aaaatacaac tgcttataaa gttagagata gctataacaa
    10381 taaatgtgat gtattggtgt taaatggata tccaattaaa acagatatga ccctttgtga
    10441 aaatgataaa ttaacactta tacaaaaagg agttaagcca tcacttgatg aattggaaag
    10501 cttgttgatt gctagacata ctccaaatat acataataaa ttaaaaaaag ggaaagttgc
    10561 tatattggga ttggggggac ttggttccaa tatagctatc tctttagcta gaataggagt
    10621 gggagaactt ttacttatag attatgatgt tgtagaacca tctaatttaa atagacagca
    10681 gtattttata gatgatatag gaaaactaaa aactgatgcc atgatagaaa atataaaaaa
    10741 aataaatcca tttataaaat taaataaacg agatattttt ttaaacaaga acaatatgga
    10801 tactattaag gattcagatt taataattga ggcctttgat gatgcttcat gtaaagcaca
    10861 ggtatgtaat tatgtattaa taaatttaaa agataaatat ttaatagcat catctggtat
    10921 ggcaggttac tatgattcaa atataataac tactaagaag ataaaagaca ggttttatat
    10981 atgtggggat tttgtgaatg aagctaaatt tggtgaaggg cttatggctc caagggttgc
    11041 catctgcgca aatcatatgg caaatttggc aacgcaaata cttatagata tgtaaatatt
    11101 aaaattagat ataaaaaaga ttgaatatta aaaaaattta acgtggaggg aaataatatg
    11161 gataaattgg tacttggtgg acatgaattt aatagcagat tattggttgg aacaggtaaa
    11221 tatggttcaa ataatatatt gcctgaggta ataaaagaaa gtggaagtga aataataaca
    11281 atggcactta gaagagtgga tttagacaat aaacaagaaa acatattaac gtatattcca
    11341 aaagagatga caatacttcc aaatacatca ggagctacta atgcagaaga agctgtaaga
    11401 atagctagaa tatctagaaa aatgggatgt ggagatttta taaaaataga agtaatatca
    11461 gatactagat atttacttcc tgataatgaa gaaactataa aagctacaaa gatacttgct
    11521 gatgaggggt ttatagttct tccatatatg acaccagacc tttatgctgg aagaagactt
    11581 atagaagcta atgcagcggc agttatgcca cttggagctc caattggttc aaatagaggt
    11641 cttcaaatga aagaaatgat aagaataatg atagatgagt tggatatacc aataatagta
    11701 gatgcaggta ttggaaaacc atctcaagct atggaggcta tggaaatggg agctgatgca
    11761 gtacttgtca acactgctat agcttcagca ggagaccctg tacaaatggc tagagcattt
    11821 aaattagctg tagaaggtgg aagagaagca tatattgcaa aaacaggaaa tgtgtcagaa
    11881 tttgcaaatg catcttctcc tttaacaggt tttttaggca atttatagaa agtggtgatt
    11941 ggtgatgagt ttttatgatg taatagaaaa atatagggat tttgattttg atggatattt
    12001 aaataatgtt actgataatg atgttttaag aagtttatca aaagacaagc ttgaagattt
    12061 tgatatatta aatttgcttt ctaaaactgc agtaaaacat ttagaagata tggcacaaaa
    12121 agcacataaa ttaagtgttc aatactttgg taaaacagta tgtttatata caccaatgta
    12181 tatagcaaat tattgtgtta atcaatgtgt ttattgtagc tataatataa agagtggaat
    12241 aaaaagaaaa aaattaacta tggatgaaat tagagaagag ggagaagtaa tttcaaaaga
    12301 aggtttcaaa catctacttg ttttgactgg agaaagtagt tttcactcaa gtgtagaata
    12361 tataggtgag gctatagaaa tattaagaga gaaatttccc tctataggta tagaagttta
    12421 tccaatggaa gtagaagaat ataagtacat tgtagataaa ggtgttgaag gattaacagt
    12481 atatcaagaa acttatgacg aggaaatata taagagagta catataaaag gcccaaaatc
    12541 aaattataaa tttagactag atgctcctga aagaggggca aaagcaggta tgaggacttt
    12601 atcaataggt gcacttttag ggctaaatga ttttagaaaa gaaacttttt ttacaatatt
    12661 gcatgggaaa tacttaaaaa cgaaatatcc tcatattgag ctttcatatt caacaccaag
    12721 aatgagacct tttaaagggt gttttgagga attggtagat ataagtgata ctgatttagt
    12781 tcaagctatg gtatgtatga gattatttga ccctcatgca gctataaata tatccactag
    12841 agaaaattta gaaatgagaa gtcatataat acctttagga gtaactaaat tgagtgcagg
    12901 tgtatctact gatgtaggag gtcattctca agatgaacat gatactgctc aatttaagat
    12961 aaatgatgaa agtactgtta aagatgtgga gaaaatgcta aactcaattg gttatcagca
    13021 tgtgtttaaa gattgggaaa gattttaaat gtatctgata acaaacagaa aattatgtag
    13081 tgaggaacga tatttagaag ttattaaaga gtctatattg agtggtgtag aaaatataat
    13141 aataagagaa aaggatttag aatatcaaga gctaagaaaa ttatatatga aaataaaaac
    13201 taaaatcaat tgtatagatt ttcaagagca aatatcagat gaaagtttga aaactaatat
    13261 aaatcaaaaa gagtttagaa ataaattcaa ggttaatttt ataataaata gtaacattga
    13321 attttttgaa aaagtggatt gtcaaggtat ccatcttcct tttaaattat tcttgaattt
    13381 aattgaaaat aagtataatt ttaatgaaaa taaaatacta ggattatctt tacataaagt
    13441 agaggaagta gattatttag aaaaattaat aagaaatcaa aatataaaaa tagattacat
    13501 aacattatca catatatatg aaactaaatg taaagaagga ttaaatccaa aaggaattga
    13561 gcttttaaaa gaagctaaaa agattactga tattaaaata attgcacttg ggggaatttt
    13621 accatctaat gtcaaggaaa cattaaaata ctgtgatgat tttgctataa tgtcaactat
    13681 aatgaggtct aaagatatta aaaaaaccat atcaaattat aatgaaaagt tgaattagta
    13741 aatatagtat attgaatttt taacttaaat aaaaataaaa aagttatctt ttaaatttta
    13801 aaaggtagct tttttatttt atatctttgc taataaaaaa ttgtaaaaag ggaaaacttt
    13861 agtcagagag attaaacaat atcctagaaa gatttaaaat ttagtaaagt atggaggaca
    13921 ttataatgac tgacagtaaa aagaaacgat ttattatacc acacactttt acaataatat
    13981 ttttactaat agtcttgatg gcaatattga cttggatagt accaagtggt gaatttagta
    14041 gagaagatgt agaaggtaga atggtagttg taccagggac ttatcaaagt gtagaaagta
    14101 atcctcaagg atttgcagat gtttttaaag caccaataga aggctttatc gattcagctg
    14161 aagttgtagg ttttgtttta atagttggag gtgcttttgg tattgtaaat aggactggag
    14221 ctatagaagc aggaatagct tcggttatct ctaagtttaa gggaaaagaa cttcttataa
    14281 ttccaatatc aatgatttta tttggacttg gaggtacaac atttgggatg gcagaagaga
    14341 cattaccatt ttatatgata tttgttccac tcatgacatc cttgggatat gattctctaa
    14401 cagcaatagc aattgtattt ataggagcag cagcaggagc agccgcttca acaataaatc
    14461 cattttcagt tggtattgca caggcactag ctcaacttgc tcctggttca ggagtaactt
    14521 acagagtagt tatttttata gcatatatac taataagtat agcatttgtt atgtactatg
    14581 ctaagaaggt aaaggaaaat cctgaaagtt caatagtata tgaaatagat aaaaataata
    14641 aatcaatact taatcaaact tcaacagaaa taaaagaatt tacttttaaa gaagtatctg
    14701 ttcttgctat atttggaata gggatggcaa taatgatttg gggtgtttta agtcaaggat
    14761 ggtacattcc agaaatatca atgtgttttg ctgcaatagg tgtgctttct ggattggttg
    14821 gtagattatc tcaaattgaa atctcagaaa gttttataga tggagcaaaa gatttagcat
    14881 ctgcagctct tgctatagct ttggctcgtg gaatcgtaat aattgctcaa aatggattta
    14941 taatagatac tatattaaat tccgcagcag cctttctagg tgggcttcca aaggttatat
    15001 ttgtatattt atcatttttc ttggaagctg cattggcatt tttaattcca tcatcttcag
    15061 gtcttgcttc acttacagta cctgtacttg ctcctcttgg tgatttggtt aatatatcgg
    15121 ctcaagtgat agtaaccgct tatcaatttg gtgtgggtct tacaaacttt ataactccaa
    15181 cttcaggagt actgatggga gcacttgcag tagcccatat tccattttca aaatgggtaa
    15241 gatttataat gcctatcttg ttgattttga cagctgtatc tctagtcttt ttaacaatag
    15301 gtatttatat agggttttaa ttaagtgtag ttttatttaa gatatttagt aatggtataa
    15361 aatgataata tgttttcaat aaattgccac taatttaatt agtggtaatt tgttttttgt
    15421 atttaaaaat aaatgtattt acttgtattt tgaattaata tctacttaga cattgtttta
    15481 attgttatat ttttatttaa ttaagcattg atttatcatt ttttatattt catataacaa
    15541 tgcaaaaaat aatttttata tagtaaatga caaaagttta caataaatgt atattttctt
    15601 actcattcct taaaattact catttagttt aataactctc atttcatatg taaagttggt
    15661 aaaaaaatta aaattaaaag taaaaaacta aataaattaa tgttaatttg aaatatatat
    15721 gaaggttttt ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc
    15781 aaatataaag gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg
    15841 gattaaaaga atttgatttt attgaagagt ctattgatat gttgaggaca atgtcaccaa
    15901 cattagaaac tatatcagat gaaatagaag aatattttga aaatatcttg gatgaaaaga
    15961 atcaagaata tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga
    16021 taattagaaa tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgttt
    16081 ctgacttaat aggcctaaga attgagtgta gatttattga ggatgaaaac aaaatatata
    16141 gacttttgag aagatatttt aataaaacag atgacaaaat aaactattat aataaagaga
    16201 ataagaatat taaattaaaa ctatctgaga ggcaacccaa taagcaaaaa aatggatttg
    16261 aaatatataa aatagatgga gtttttgcgt atttagatag agaagtaaaa tttgaacttc
    16321 aaattaaatc tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa
    16381 acaatacata tttacttgct gataaattta taaaggatat gatggactct ataaagaata
    16441 atcttactat gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt
    16501 cttttaatat gaaagtaagt aaaaaagaaa ttgaaaaatt atttgcgaaa ctagtctatg
    16561 acgctttttc tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat
    16621 gtgagactat attgaactat tcttttaata aacaagatat aagtgatgaa gttttaggta
    16681 attttatgct tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta
    16741 atgagcaaat agaatttgaa agagaaccaa catttgatga taagttttgt aaagacctag
    16801 gaaatcattt tagaagtaga ttaaacacag aatttccatg gaacctattt tttagaatat
    16861 tatttgaaat agagccatat aacaatacca aagactttga aaactttgta gagtttataa
    16921 aagaaaatgt gctactagaa gaaaataggg agcaattatt atgccaattt gaagaggata
    16981 gtaagcttat aattgaagat atatatgatt gtatatttaa tagtataagt gagattgata
    17041 gtgttgaaat tttatataac tataatcttg aaaaaataaa ctttacatca agtgaaatca
    17101 taaattgtat atgtagagaa tatgagtgtt atggtgaata cttagaagaa aaagaaaagc
    17161 ttatgaatac atttaaagaa aagattattc aaatctttga gtaataggtc aatgctgaag
    17221 tggcgctaat gcgccatttt tcaaatatat atgaattaag taaaattatt ttggaggagg
    17281 cgatttgtat agagaaaaca ggtgtaattt tggcaggggg aaaaaattct agaatgggta
    17341 gagataaagc ctttttagag ttacatgata agctttttat tgaaattgca atagaagcat
    17401 ttaaaaactt tgatgagttg ataataatat ctaacaatga agaattatat tctaaatacg
    17461 atattaaagt ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg
    17521 ctcttgaata tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata
    17581 atcaaatagt cgaaagaata gctaataaaa tggatgataa aagtgtgatt tcagtgacaa
    17641 atggaaagtt acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt
    17701 tatgtataag ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagtt
    17761 atatatactt tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtataaaa
    17821 aattaactaa agtttaatta gctatggttg taaaatagag taggatttat atggaggaga
    17881 gtttatgaat ttacttaaat gcgattcttg ggcagtaata ttaaataata gcgataaaga
    17941 aagtaaagca tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat
    18001 agatgaagag aaaaaaccta ttgaaggaat agatgtgtat gaatgtttaa aagatgttcc
    18061 acacaatatt gatgttgtag ctattattga taaacaatct aagatggatg tgattttaga
    18121 agaagtagag cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat
    18181 gatgattaaa aagactaaag atttaaaatt aaatatagaa tataatttaa gtttatatga
    18241 tgagttgact agataaaatt atatttctta ataataaaaa tataattagt taataaaaca
    18301 tattatttta tagtatagat tggttaataa aagtacagat tggttaatga aacattaaat
    18361 tacaatattt gttaaaaaaa ttgtgagtta catgatataa tgtattaaat aggatactaa
    18421 ggagttgata tattatgtta atagtaacta cagaaaaagt agaaggtaaa aagatatcaa
    18481 aggttttagg attagtgaga ggaagtacaa taagagcaaa acatgttgga aaagatatag
    18541 gagcaagttt taagaatctt gttggaggag aacttactgg atataatgaa atgctcactg
    18601 aagcaagaca aattgccata ggtagaatgg ttgaagatgc agaagctaaa ggtgcaaacg
    18661 cagtaatagc atttagactg tcttcagctt cagttatgca aggagcagca gaaatgcttg
    18721 cttatggaac agcagttgtt ttagaagatg ataatagtat tcttgaaaaa taaaatataa
    18781 atagttaaaa agaggcatct tttaaaaatt ttatggatgc ctttttttat ttgaaaaaac
    18841 taatttgaga agaaatttta actattatga caataaaatt agggtgatat tatataaata
    18901 agtaaaatta tattatataa tgtatggtat agactttaaa aattttattt gtagagaaaa
    18961 atatggacaa tgttataata attatagaaa ttaaaagtac ttacattcat tatgtatgtg
    19021 acatgtataa ttagttttat atttaataca gttttgaagt aatatcttca ttttgagaac
    19081 gttggaataa taaaaaatgt attattatag gtgatataaa taaaaaataa atataatatg
    19141 ttatattgct gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat
    19201 ttataggact taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa
    19261 cacttagtga taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg
    19321 aatttgttga aaatacagga gtttataaag ttactgaata tgtacttata aaagatgata
    19381 aggaaatgct aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc
    19441 ttacaaatgg aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag
    19501 tgttggaaaa agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta
    19561 ctaaaaaggc catactaagt aggggtgtga gtggaataag aaataattct ataataataa
    19621 atttacctgg aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag
    19681 accatggcat agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat
    19741 agtatataat ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag
    19801 tatggaagaa aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt
    19861 gtctattgta tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc
    19921 tttgaagact acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga
    19981 tttactggag gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa
    20041 gaagaatgtg gaataaataa cataggaatg accactaatg ctattggtct ttctgataga
    20101 atttttgagc ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag
    20161 gaatataagt ataaatccat aacaagagga ggaaacttaa aagatgtctt aaaatcgatt
    20221 gaatcttgtt taaatttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt
    20281 aatgatgatg agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga
    20341 tttatagagc ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat
    20401 gtaaaagaaa tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt
    20461 acagcagaat attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta
    20521 agctgttcat tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa
    20581 ttatgtctac attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta
    20641 tttagagaat ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa
    20701 aataaatgta gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca
    20761 catataaatg aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa
    20821 agaacggccg tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa
    20881 aatggtcaga taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga
    20941 gctaaaaata catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat
    21001 ttagaatttg atgtagatga ttctatgtct gctattaaga tatacgcaac ctgtaaatct
    21061 cttgggaaaa ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca
    21121 atttatgata tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgtg
    21181 gagaagactg gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt
    21241 taaaatttgg aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaagggtg
    21301 taattaaagt tcctgtacaa tgtgcagagc taaaaataaa tcatgggata gttggagatg
    21361 cacatgcagg aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa
    21421 tgaaaatgaa aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag
    21481 aagggataga agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag
    21541 ttgaagtaac tcaaataggg aaaaaatgtc acaatggatg tgaaattaag aaattgacag
    21601 gtgactgtgt gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa
    21661 atgtgaatga ttcaatagat gtaatctaat gaagtcacta ttaaaatata attataagta
    21721 aatactatta agatttaaat atatatttta agagagaatt aattttaata aaatatttta
    21781 gttataaatt ggaatattgg ttatataaag ctatgaatgt aaaatcatag ctttt
//
LOCUS       DAYAOY010000069        21810 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYAOY010000069 DAYAOY010000000
VERSION     DAYAOY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.069"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..213)
                     /locus_tag="ACGI6B_002651"
     CDS             complement(<1..213)
                     /locus_tag="ACGI6B_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752086.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIG"
     gene            376..549
                     /locus_tag="ACGI6B_002652"
     CDS             376..549
                     /locus_tag="ACGI6B_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752087.1"
                     /translation="MNDATKVSIKNETNNIMNLCNLLSWNALLQNNKINAETIAKSRY
                     FIIILSPIDFYRL"
     gene            complement(561..836)
                     /locus_tag="ACGI6B_002653"
     CDS             complement(561..836)
                     /locus_tag="ACGI6B_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752088.1"
                     /translation="MTLLELYYRKAEINKIVWARIGFNLYLSKGVALTLTDAGIGISG
                     LLAGAIPGIGQYLPLIVRLITMYLESNITSGKIFKFKKYVIIKEVLV"
     gene            1272..1661
                     /locus_tag="ACGI6B_002654"
     CDS             1272..1661
                     /locus_tag="ACGI6B_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752089.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            1981..2490
                     /gene="vanZ1"
                     /locus_tag="ACGI6B_002655"
     CDS             1981..2490
                     /gene="vanZ1"
                     /locus_tag="ACGI6B_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HFG9752090.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(2592..2786)
                     /locus_tag="ACGI6B_002656"
     CDS             complement(2592..2786)
                     /locus_tag="ACGI6B_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HFG9752091.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(3302..3694)
                     /locus_tag="ACGI6B_002657"
     CDS             complement(3302..3694)
                     /locus_tag="ACGI6B_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9752092.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            4261..4623
                     /locus_tag="ACGI6B_002658"
     CDS             4261..4623
                     /locus_tag="ACGI6B_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752093.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            4607..5332
                     /locus_tag="ACGI6B_002659"
     CDS             4607..5332
                     /locus_tag="ACGI6B_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752094.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            5352..5771
                     /locus_tag="ACGI6B_002660"
     CDS             5352..5771
                     /locus_tag="ACGI6B_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752095.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            5761..6285
                     /locus_tag="ACGI6B_002661"
     CDS             5761..6285
                     /locus_tag="ACGI6B_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752096.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            6597..7154
                     /gene="efp"
                     /locus_tag="ACGI6B_002662"
     CDS             6597..7154
                     /gene="efp"
                     /locus_tag="ACGI6B_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFG9752097.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            7246..7701
                     /locus_tag="ACGI6B_002663"
     CDS             7246..7701
                     /locus_tag="ACGI6B_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HFG9752098.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            7864..8574
                     /gene="rnc"
                     /locus_tag="ACGI6B_002664"
     CDS             7864..8574
                     /gene="rnc"
                     /locus_tag="ACGI6B_002664"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFG9752099.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            8628..9698
                     /locus_tag="ACGI6B_002665"
     CDS             8628..9698
                     /locus_tag="ACGI6B_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HFG9752100.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            9726..13280
                     /gene="smc"
                     /locus_tag="ACGI6B_002666"
     CDS             9726..13280
                     /gene="smc"
                     /locus_tag="ACGI6B_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFG9752101.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            13307..14587
                     /gene="ftsY"
                     /locus_tag="ACGI6B_002667"
     CDS             13307..14587
                     /gene="ftsY"
                     /locus_tag="ACGI6B_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFG9752102.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            14690..15058
                     /gene="ylxM"
                     /locus_tag="ACGI6B_002668"
     CDS             14690..15058
                     /gene="ylxM"
                     /locus_tag="ACGI6B_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HFG9752103.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            15055..16407
                     /gene="ffh"
                     /locus_tag="ACGI6B_002669"
     CDS             15055..16407
                     /gene="ffh"
                     /locus_tag="ACGI6B_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFG9752104.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            16445..16717
                     /gene="rpsP"
                     /locus_tag="ACGI6B_002670"
     CDS             16445..16717
                     /gene="rpsP"
                     /locus_tag="ACGI6B_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFG9752105.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            16782..17009
                     /locus_tag="ACGI6B_002671"
     CDS             16782..17009
                     /locus_tag="ACGI6B_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFG9752106.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            17117..17632
                     /gene="rimM"
                     /locus_tag="ACGI6B_002672"
     CDS             17117..17632
                     /gene="rimM"
                     /locus_tag="ACGI6B_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFG9752107.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            17641..18336
                     /gene="trmD"
                     /locus_tag="ACGI6B_002673"
     CDS             17641..18336
                     /gene="trmD"
                     /locus_tag="ACGI6B_002673"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFG9752108.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            18521..18871
                     /gene="rplS"
                     /locus_tag="ACGI6B_002674"
     CDS             18521..18871
                     /gene="rplS"
                     /locus_tag="ACGI6B_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFG9752109.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            19192..20088
                     /gene="ylqF"
                     /locus_tag="ACGI6B_002675"
     CDS             19192..20088
                     /gene="ylqF"
                     /locus_tag="ACGI6B_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFG9752110.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            20477..21769
                     /gene="brnQ"
                     /locus_tag="ACGI6B_002676"
     CDS             20477..21769
                     /gene="brnQ"
                     /locus_tag="ACGI6B_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9752111.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         9141 a         2079 c         3838 g         6752 t
ORIGIN      
        1 tcctattgct atatattcaa aacctgttac atatcgataa agtacttcta gtttaatagt
       61 tctttttcta tctatatttc catattttga ttctaattta ttaaaatatt tttcattaca
      121 aaaaccaatt ttaatttcaa ttaaagatat tattgtagtt aatattataa ttcccaatcc
      181 cataagtata agataaatgt tcctaaaatt catagaagat acctccttta taatataatc
      241 atcaactcat gttgtattaa ttttcctaat caaagtataa cagtaacaca agtataaaga
      301 gtatctaaaa tattacaaat attaaatgag aaaaagatgg taagttaacc ccaccatctt
      361 attttttatc tactaatgaa tgatgctaca aaagttagta taaaaaatga aacaaacaat
      421 ataatgaact tgtgtaattt acttagttgg aatgctttat tacaaaataa taaaattaat
      481 gctgaaacaa ttgcaaaaag tagatatttt ataatcattt tatcacctat tgatttctac
      541 agattgtaag tagtatttgg ttacacaagc acctccttta ttataacgta ttttttaaat
      601 ttaaagattt ttccagatgt tatatttgac tcaaggtaca ttgtaattaa tctaactata
      661 agtggcaagt actgtcctat accaggaata gcaccagcca aaagtcctga aatccctata
      721 ccagcatcag ttaaagttaa tgcaactcct ttagaaagat ataaattaaa acctattcta
      781 gcccaaacta ttttatttat ttcagctttt ctataatata attcaagtag tgtcaatatc
      841 gttcttctta aggtttttaa ttagcttata tattgttatt gtataatata attattaaaa
      901 attaactata ttaaatatta ataaaaaata tagtaaaact taataataca tataataaaa
      961 cttaatataa attaagtata tgaaataaat atcctgtatt taagcttata gattataatg
     1021 aaatataata catgttgcaa taataaaacg aactaaaatt atcacactaa agcataaaac
     1081 atactctaat catgttagat aaatttttga atatataatc caaaagtact ctctaaagta
     1141 gagtacttct tttttattaa ttatttttat atagaataaa ttggaattta tatttattcc
     1201 aatctaattg attacataca cttattgtta taaagtttag ttatacataa attggtaaga
     1261 ggtgtaaact aatgaagatt aataaattac cacatgcaga actaaaacta atgaagtata
     1321 tttggggagt agatgatgta ttagcctcaa gagatatcat agaagacatg aagttgaaat
     1381 acgattggaa aaagagcaca acattgacat ttttaaaaaa tttagtagat aagggctttt
     1441 taactactga taaggtagat agatgtacgc attatactat agctataaaa gagaaagatt
     1501 acttaaaagt tgagacaaag agttttttta gttttatgca taacaattca tttaagagtt
     1561 ttatttcagc actacatgat gatgaagttt tagatagtaa gagcttggac aaattagaag
     1621 aatactttaa aaatttgaaa gaagaagata ttgatgacta ataaagctat cagtatcttt
     1681 ttagtttgag acaattgaga gtagcgtttg gatttaatgt tagttttatg tgatggtaga
     1741 acattatata aagaaaagtg attatttcct tagtgtttga tagtaaaaaa tatagagaat
     1801 aataccaatt aaaatttaaa gatatatgaa atactacaat agcttaaatc atgtaaatat
     1861 gataaagata ttgaaattat aaaaggaata taattttaga agaaatagtt gacagataaa
     1921 ttaccttact atattattgt attaataaaa taatacaata atatagtaag gaggtaataa
     1981 atgaaatcta gaaaacataa tattacaaaa ggattattta tagtttacat aattatttta
     2041 acatggataa tactatttaa gttgcaattt gatatctcat ctcttgaaac catgaatctt
     2101 agaagcataa acttaatacc ttttgctggg tcacttataa ttaataatag agtagatata
     2161 tcagagatta tattaaatgt ggctatattt gtaccatttg ggatatatgt gtgtatgcta
     2221 aaagaagaat ggagctttat aaaaaaagtt atacctattt ttataactag tttggtattt
     2281 gaaacacttc aatatatatt tgcactaggt gcaagtgata taacggattt gataggaaac
     2341 acactaggag gaatcatagg aatagctgtt tttatgctat tatcgaaaat atttaagaat
     2401 aataccataa aaattatcaa tgtattggca ttgattgtga caattattgt tgtattattt
     2461 ttaggattag ttatttttgc aaacttatag aaataagtat atatgtttag cttaaaatag
     2521 aacgtaagat acagttacat ataaaattat ttacacataa aaagcaccta tacaggtgct
     2581 ttcctaaatt attaaagaat tattgtatct tgaaactctt gtatgttcaa ttcttgattt
     2641 tctcttgttt tttctaataa atctaacgca caatttatac aaactttaat ttctgcattg
     2701 gtatttttct cataataagt tatatattta tttctatatt tatctatttc ttttccacaa
     2761 cgactacaca ttacaattac gctcatatct tagttcctcc atctctaatt attcttaata
     2821 tattcaatag tatcactttt ctgaaaataa tgattgttaa aactgtatca aatttagatt
     2881 tactttttat tataatgcat tttaataaaa aaataaaggt tatgggtaaa atatttatag
     2941 aaaaaagtat attattgaat agattataaa attttattat tttttagcag tgttaaatac
     3001 acattaatat ctataaagtg acaaaataaa aaatactatt taaatagata ttaaaaaatt
     3061 gataccaagt atttatttgc tatatgaata acaatttatt taggaacttt actttaacac
     3121 tgtataataa taaagtaatt gctaaaaacg tttgatgatt tggatgtatt tttagaaatt
     3181 agaaaaatat atacttactg actaatgata atcttatatg tattttagtt tgagtgattt
     3241 aagaaaagtg ttttgaatgg ggattatttt atagagtaaa taaatatata atagtagaaa
     3301 attacatata aagagtagtg tttatttcat taccttcttt atctgttccg atagggtctt
     3361 gtagagatac ttgagtttta ttttttttgt tagatctaat attcattaaa atctcatttt
     3421 caatacatct agaagcatat gttgctaatc gagttccttt atctatgtta taagtttcta
     3481 tagctttaat taatcctatt gtacctatgg atattaaatc atcctgatct tccgttgaat
     3541 tattatattt ctttgctata taagctacta atctcatgtt tctttcaatt aatgtatctt
     3601 ttgcactttt atcgttttct attttaaatt tagttaaata ctcaatctct tcttcagggg
     3661 ttaagggttt ttcaaaagat ttaagagctg ccaataggtc accccctttt caacggtcac
     3721 tttaaattat atgaaggaga cttatccaag ttgactgtta tctaataaaa taatatgcat
     3781 atttttagaa tttaaaacat atatttgcaa acttttgaat ggaatatgac ataaagagtg
     3841 ggaatttaaa tttaagtttt tgttgtatat atatgaaata aggcgaagaa attattgatt
     3901 tataaagtat aaattggaat tgaggtgtca ataaatataa aaggaaatag tatttatata
     3961 tgttttggtg ttgaagtaaa agattaatgc aaagcattag agtctataaa aatatgaata
     4021 taggaggtgt aattttacaa tacttaaaaa agtaaatgta attaattgtt tgtataaagt
     4081 tgattacaag caaaagtatc agtcatagtt aatactgtta agggtgaagg tggtgtgtta
     4141 atttatatat gtatgggaag aaattttgat tacaatgaaa aaacttacaa aagatattta
     4201 tgaaaaaact tatagttaaa tgtttatata taaaatatta tttaggggtt gtttttatta
     4261 ttgaaaataa gaaagagtgg ttttatatca attgaatgta taataagtat tgctatatta
     4321 tatgtggctg tttatttagt ttctacatca ttgtataatt gttatagttt tatcagtaga
     4381 aatatatctg acagagaaat gttaagtaca gcaaaaaaat atatagaaga tgagaagtat
     4441 agaatacaaa atagtaagta tgagttaatt gaagataaga tagaaaaaaa ttacataaat
     4501 ggatatgaaa ttaacagtaa aatagagcaa attttagatt attatcaatg ctatgaaata
     4561 aatatagaga taaaaaatga atttaaaaaa ctgaggttta atagctatgt tactagaaaa
     4621 taaaggctct atattaataa taacattgat aatattttct attataagta ctgtgtgcat
     4681 tatgtgtata ggtttaattt atacacataa taatatgttt gagttaggat acaaggacat
     4741 tcaactaaaa gaaatttctt taagtggaat agaaatatca gtaagtaatg tactttcttg
     4801 tgttgaggaa gctattgatg tatctaataa tgaagacgaa tttagagaat atttcttagg
     4861 aaataatatg attgaatgta taaaaaaaat aaaagataca tcttatagta gtttagaaga
     4921 actttcttta aaagtagaaa ataatactat ttatgacaga ggagattttt ttgaatttga
     4981 tatagaaagt aatgttagag aaaaagaatt tttaaaagca ataaaagtaa gagtgaaaat
     5041 caaaaatcca tggataggaa ttgacattga agattatatg gataaatcta ataacaataa
     5101 aattgatgac aaaacattca ataattataa agcatctgaa aataatgaaa ttctaaatca
     5161 atttaaaaat gaaagtgaaa atataaggaa tcaagaggtt ggtggattta cagaagaaga
     5221 tgaagaagat gaagaatatg aagaagaact taaacaaaac ataattgaaa atataaagaa
     5281 gaattttgat gaaaaaaacc tagtacaggt atataagtat aggggtgtat aactttgaaa
     5341 aaagaaggaa tgtgactgta atagaattaa tagtatctat cagtataata ctcttgatag
     5401 ttacattatc ttttccaaaa gataatcttg aaaatcataa aataaattta tttgcacgtc
     5461 aactttgttc agatataagg tatgttaggc gaattaatat gtttagcaac tacaatacat
     5521 atatatatta tataaatcaa ggtaaacacg aaggttatgt attgagaaaa gatggtaaaa
     5581 acattaaatc aataatgcta cctaaaaatg caaaaatagc taggagtatt gaaataataa
     5641 agtttaaatc tgatgggtcg cctcaaaagg cgtctacaat agagatatat aataatagat
     5701 taagaaaaac tataacaata acacctgtaa gtggaagagt acttctgaaa gagggaaaat
     5761 atgaaacata aatatggtta cttgcttttg gaaagtgttg tatcattgtc ttcaatggtg
     5821 ataattatac tagttttata ttctatattt ttgtctacaa taaacttaaa gttaaaagta
     5881 gaagataaga tagaactaca acaacaaagt cttgaaatca taaaatctat ggaaggaatt
     5941 attagcaatt ctatgggaat aatgaatgta tctaattatg aagaaacatt taaaaagaca
     6001 acatctataa aatgtagata tttagatgaa aataataatg aagaaggcat aagtaataaa
     6061 gaaattatat tgaatgaaag acgaaataag ttatttgtaa attctttaaa tggcgaaagt
     6121 agtcaagcag gtggttatga aattggagat tatgtagatg agatgtatgt atcaattact
     6181 aataatggtc aatatgttaa tattaaattg aaattatcaa aacgtagtca aaaatatgag
     6241 actgatttca aaataaaagt atggaatttt agtgaaagta tatagaaata cataaattta
     6301 agagaaagaa gtgacatcaa taagtcatat aaaatataat tattgagaac tatataacta
     6361 aataatatca atatgtaaag tgtatagatt aagaatatag aggataataa taaaaatata
     6421 gaataagtaa tttgccaaat acttgtttat attgtataac ttatgatatt atgatattag
     6481 taaaaatttg taatcctgac ttgacagaat tcaagtaaga gatataattt taaaggcata
     6541 gtaatgtaaa atttactaaa aaataagaaa tttattttaa tatggaggaa caaaaaatgg
     6601 tatcagcagg tgattttaga aaaggtgtta catttgaaaa agatggacaa ccatgtttag
     6661 tagttgattt tcaacacgtt aagccaggta aaggagctgc tttcgttaga actaaataca
     6721 aaaacttaaa aacaggagct ataagagaag aaagtttcaa tccaagtgaa aaattcccta
     6781 aagctgttat agatacaaga caaatgcaat atctatacaa tgatggtgag ttatattatt
     6841 ttatggatca agaaaatttt gaacaaatac cattaaacta tgagcaagtt gaagatgcaa
     6901 ttaagttctt aaaagaaaat gaagttgcta caataagatt ttaccaagga caaccattcc
     6961 aagtggaagc gccaaacttt gcagagctag aagttacaga tacagagcca ggtataaaag
     7021 gtgatacagc aagtaatgta actaaagcag ctacagttga aactggagca gttgttcaag
     7081 ttccattatt tataaatact ggagataaag taaagataga tactagaaca ggtgaatatt
     7141 tatcaagagt ataaaaattt atgtatataa aagcttatat gtcttaataa tataagatat
     7201 atgagctttt agtttatata taaaaaagaa agcgaggggt gttacatgaa acatttatat
     7261 gaagaagttg catacttaaa aggcctagca gaaggactag aaataagtgc tgaaagtaaa
     7321 gaagggaaaa tgatacataa aatagttgat gcattagaag tatttgcaga tgctattgta
     7381 acattggatg aagaacaaga agaacttcaa gattttgttg aatctataga tgaagattta
     7441 gcagatttag aagaagatat tgctgacatg gaagaagatc tttatgaaga agatgacgat
     7501 gaggatgatg aggacttcag ctatatagaa atggaatgcc caaattgtgg agagttagta
     7561 gaaatagacg aagatttgtt atatgatgat gaagtagatg ttgtttgtcc tgattgtaag
     7621 gctgtgatat tatcatcaga agatgattgt gatgatgatt gtacatgtgg tggttgttca
     7681 agttgtgatg atagagagta aaaattcata agaattaaaa ctgcctatga tgcctatgag
     7741 atattttgct tcataggtag ttttattttt atgaggaagg gtattatgta ttttggtttt
     7801 gcaatacttg gacaaaaata gtacactaga atttatagaa taggattttg gagggatgta
     7861 aatatgaaaa taagtaaaaa attactagat aatatacaaa gatttgaaaa tgttataaat
     7921 tataaattta aaaataaaga atatatacta gaagctctta ctcatagttc atattctaat
     7981 gaaaataaaa aatataattt taacgaaaga ttggaatttt taggggattc ggttttaggc
     8041 atagtgataa gtgattattt atttaatgaa gaagctaatc tgcccgaagg ggaactaact
     8101 aaacttagag caaatatagt ttgtgaagat tcattaagtg aagtagctaa tgatataaat
     8161 ttaggtattc acatgttatt aggtagagga gaagaagcaa caggaggaag acataggact
     8221 tcaatattag cagacgcatt tgaagctgta atagctgcta tatatcttga tggaggtttt
     8281 gaaagtgcaa gacagtttat acttcatcat atggaaaata taatttatga ttcaagaaaa
     8341 ggaaatatat ttagagacta caaaacgcat cttcaagagg tgttacaagg gaatggagaa
     8401 aataatatct ggtacaggct tatagaagaa aaaggaccag accataataa aagatttgta
     8461 atggaagttg gaattaatga cgatgtactt ggtataggag aaggcaagag taaaaaagaa
     8521 gctgaacagt tagctgctaa aatagcttta aagaaaaaat tatgggaaaa ataaatataa
     8581 tatgtaaaaa tataaaaaga aatatttata ttagaaggat gttgtagatg aaaaagagaa
     8641 ttatacctat atttgttcct cacaaaggat gtcctcatga ttgcatattt tgtaatcaaa
     8701 aaaaaataac aggtgtgagt actgatgtta ctagtgaaga tgcaagaaat ataattgagg
     8761 aatgtttaga gactatagat aaagatgcag atgtagaaat agcatttttt ggtggtagct
     8821 ttactgctat agatattgat atacaaaaaa gtttattatc agttgctaaa gaatatgtag
     8881 aaaaaggtct tgtaaaagat attagaatgt ctacaagacc agattgtatt gatgaagata
     8941 tattaagtat gttaaaagaa tataagacaa gtataattga actaggagta caatccttag
     9001 atgaaaaagt attattagat agtataagag gtcatcaatc tgaaattgta tacaagagtt
     9061 caaagatgat aaaaaatagt ggaatcaaac ttggattaca gatgatgatt ggtcttccag
     9121 ctgacacaga agaaaagtgt atatttacag caaaaaaatt tgtagagcta aaaccagatt
     9181 gtgttagagt ctatccaact ttggtagtaa aagatacagg tcttgaaaag ttaatggaac
     9241 aaaataaata taatccattt actcttgaag agagtgtaaa tatagtaaaa aaagttcttg
     9301 tactgtttta tgttaataat atcaatgtaa taagagttgg gcttcaagct actgatgata
     9361 tacaaattgg aaaagctgtt ttagcaggac catatcatcc tgcatttaga gagttggtag
     9421 aagcagatat gataaaagat tatttagaat ttgttatttt gcaaaataaa aatataaagc
     9481 aaatgttggt caaatctaat aaaaagaata tttctaaaat cataggaaat aaaaaaacaa
     9541 atgttaaata tatggaagaa aaatttggtg tgcttttaaa aacacaagaa tcagatttag
     9601 atataaatca attagaaatt gtgttagatg ggaaaagttt aattagtgct aatatgagag
     9661 acattcatag aaaattgtat gacatttatg acctttagtt ttaataatga agggagagaa
     9721 aagatttgta tttaaaaaga ctagagttaa agggatttaa atcctttcca gtaaaaacag
     9781 atattatttt taaagaagga ataacagcca tagtaggacc aaatgggagt ggaaaaagta
     9841 atatatctga tgctgtaaga tgggtattag gagaacagag tattaagagt cttagaggtg
     9901 ataaactcga agatgtaata tttgcaggta cagatacaaa aaaacctatg aattattgtg
     9961 aagtggctct tactattgat aattctgaaa atcaattgga attagatttt acagaagtaa
    10021 ctataagaag aagggcttat agaaatggtg aaagtgaatt ttttctaaat aataaaagtt
    10081 gtagattaaa agatataaaa gaagtctttt tagatactgg tattggaaaa gatggttatt
    10141 caatcataga acaaggaaaa gtagatgaaa tattaagtaa taatccttta agcagaagaa
    10201 aagtttttga tgaagcttgt ggcatatcaa aatatagata taaaaaacag gaagcagaaa
    10261 ggaatttaag taatacaaag gaaaatttag aaagaataga tgatgtatat atagaaattg
    10321 aaaatcaatt aaagcctctt tttaatcaac agacaaaggc aaaaaagtac ttggaaataa
    10381 gtgaaaaatt aaagacactt gaagtaaata gctttataag agaaattgaa ggaatagaaa
    10441 aagaattaag tgaggttaat gaacatcgta aggttattga aaaggagctc aatgaaaaag
    10501 aagaacaaaa aaatgttgta gagaaaaagc aagaagatat taataaagag gtagaggtat
    10561 tacaagatgt aattgaaaaa tctgtagatt atataaattc aattaaagga gttatatcaa
    10621 aaaaagagtc ccaaataaat ttaattaagg aaagaataag aaattttacg aatgaaatta
    10681 gtagaaaaaa tttagaaata aaggatataa aagagaagct aaatgaaaat aagcaataca
    10741 taaaagaatt agaaagcaat aaattatcag gtagtgaaga actatctacc ttgcaggaaa
    10801 atataaaagt attggaagga agtaaagata aacaaaaaat taagttagaa tccctaaata
    10861 atgagataga attattgaaa gaaagtataa ttgatatatt aaataagaaa caagagttta
    10921 gtaataagct gtctacttta aacgcaaata aagaaaatat gaatatcaga gatgaaaata
    10981 taaattcaga gataacagag ttaaacaaaa atattgaaat aaaatcatct gaattagata
    11041 ctataaataa agaatttaat atgcaaaatg aaaatttaaa gaatgtaaat aatagacata
    11101 aagaattatc aattaatttg caggattcaa ttagtgaaca caataaacta gaagatgaaa
    11161 tacagaaaag taaatataat ttaaatggat ataattcaaa gttaaatgtc tatatagata
    11221 tggaaaatca ttatgaagga tttaatagag gagtaaaaga agtactaaaa aataaaaatt
    11281 taaaaggagt tcatggagca ttaggacaga ttataaatgt acctgaaaag tatgagaaat
    11341 ctatagaagc agctcttggt gcttatatgc aaaatataat aactgataat gagtttagtg
    11401 ctaagtttgc aataaactat ctaaaacaga ataatttagg tagagtaaca tttcttccat
    11461 taaatataat taaatcaaat aaaataagtc taggaaattt aaaagcaaat actaagttca
    11521 taggaatagc aagtgattta attacatttg atgaaaagta tagaaatata attgaaaata
    11581 tacttggaag aacaatactt ataaataata tagatgaggg cataaaattt gcaaaagaaa
    11641 ctggtcatag gtttaaaata gtaactttag atggtgaaat cttaaatcca ggaggttctt
    11701 tgactggggg aagtctaaaa actaatggga atatactatc gagaaaaaga tacattaatg
    11761 aatatacaga aaagataagt aatatcaaga atgaaatttc acatctagaa ttaaaaagag
    11821 aatctttaga caaagatgtg aaaaatatca aaaatgaaat tgattcacat gaaagtaaaa
    11881 taaaagattt agaaaaaagt ataataataa aaagtacaag tataaagaat gttgagtctg
    11941 aaattgaatc gttaaaaggt agtattacta aattagaaaa tgagaagaat gatttaaatt
    12001 ctaacctaaa ttatacactt gaaaaaagtg atgatgttag aaaagatatg gaagagttag
    12061 atgatttata caataaaaat aaagaaaaaa ttgatgcatt gaatgaggaa ataaaaagat
    12121 acaatgattt atatgataaa gaaaaatctg aatttgatga attgaactta agtttagtga
    12181 agaaaacaga agtttacaat agtatagtta gagatataaa aagaatatct ggtgaaaatt
    12241 gtgagctaga agaaaaaaat aaacaattag aagagtcatt aaattatgaa gaacatgaaa
    12301 taattaaatt acaagattct atactcactg aagaaaaaga aaaagaaaat ttaacaaaac
    12361 aactagggga tagtaataga aatcttgaaa ctagaaaaat agcaaaagat gatttaaaaa
    12421 atagttttga tgaaattaat aaagaattaa aaactataga tagacagcat atagaactta
    12481 aagaaagttt atttaaagtt ggtggaagat tagaaagatt aaaaactagt caagacacat
    12541 atataaataa gttgtttgag caatatgata tgaccttagt acaagcattg gaaattaaag
    12601 atgaagattt ggatatagat agaaaattct tagaaagtct aaagagagaa ataagaagtc
    12661 ttggaaatat aaatatagac tctataaagg aatatgagga aataaaagaa agatacgatt
    12721 tttatagcga acaaaagcaa gatttagaag agtctatgga agagatagaa aaacttatac
    12781 atactttaga agaaaatatg aaatctgaat ttgaaattaa gtttgaagaa ataagtaaaa
    12841 attttaaata tgtatataag agattatttg gtggaggttg tggagaatta actattttag
    12901 ataaagaaaa tttattagaa agtgatatac taataacagc tcaaccccca ggtaaaaaaa
    12961 tgaaaaattt aaacctatta tctggtggag agaaagcttt aacggcaata agtatattat
    13021 ttgctatatt gattacaaag ccaacaccat tttgtatatt agatgagatt gaagcgccac
    13081 ttgatgacgc aaatatattt agatttggag aatttttgaa ggatttatct aaagagactc
    13141 aatttatatc agtaactcat aggagaggaa caatggaagc tgctgattat atctacggag
    13201 ttacaatgca agaaaaagcc atttcaaaag ttattagttt aaaattaaaa gaagctcagg
    13261 aaataacaga tattatataa cttagattga cataggagga ataacagtgt taaaaaaatt
    13321 atttggtttt ggaaaagaca aggaaaagga aatagaaaaa aaagatgctg aagaagaaat
    13381 agaagttgaa gacagtgttg ataatttaga aaacttggaa gaaactattt tttcaggact
    13441 tgaagaagaa gtgatagata aagtggaaga tattgaagaa aaaaatgagg aaagtgtcaa
    13501 taatgaaggc actgaagaaa tcgaaaactt tgaaaaaata gaagttgaga tggatagtaa
    13561 tggaaaagaa gtagaaaata tttcaaataa tgattctcaa gaagagataa tcgaagaatt
    13621 agaaaattat gatgaaattg agtctaaaga gatagaagat aaagaagata agaaagttaa
    13681 cttgtttgaa agactaaaac aaggattgac aaaagcaaag caaggaataa ctgatagaat
    13741 agatgaagtt ttaaaatcat atacaaaaat agatgaagaa ttacttgaag atttagagga
    13801 aattttaata acagcagatg ttggtgtaaa tacaactatg gatataattg aaagactaag
    13861 agataaaata aagcaaaaag gtattactga accaataaag gtaagagaag aacttaaatc
    13921 tatagttgaa gatattctta caaatgaaaa ctctacacta gatatagaac cagcaccatg
    13981 tataatatta atggtgggtg taaatggggt cggtaagact actacaatag gtaaactagc
    14041 aaatagatac aaaaaagatg gtaaaaaagt tttattagca gcagcagata catttagagc
    14101 agcagcgaca gagcaattag aaatttgggc aaatagaacc aatgtagaca taataaaaca
    14161 tcaagaggga gcagaccctg gagctgtagt atttgatgct ataaaagctg caaaagctag
    14221 aaaaacagat gtattaatat gtgatacagc aggaagatta cacaataagg ctaatcttat
    14281 gaatgaactt ggaaaagtat ttaaaatagt agatagagaa tttccagaag caaaacgtga
    14341 agtacttctt gtggtggatg ctacaacagg tcaaaatgca gttgttcaag caaagacatt
    14401 taaagaagtt gcagatataa caggtatagt acttacaaaa cttgatggaa ctgcaaaagg
    14461 tggagtagtc cttgcagtaa aatcagaagt agatgtacct gtaaaactta ttggagttgg
    14521 agaaagtgta gaagacctac aagatttcaa tgcaaaatca ttttcagatg cattatttgg
    14581 taactaatgt tcttttaaaa ataatggttg acaagagtgt tgttatgata taaaatacaa
    14641 tagtgtaaag taaataacct ttacagtata ctgctaggaa gtgtaagata tgaatataga
    14701 aaaaatggtc gaaattggat tgttatttga acagtataaa gaattattaa ctgacaagca
    14761 gaaagaaata gttgctctat actatgaaga agattattct ttaggtgaaa ttagtgaaaa
    14821 tctgaatgta tcaagacaag gtgtttatga taccttaaag cgttcagaaa aaatactaag
    14881 agattatgaa gaaaagttac acttagtatc aaagattcaa gagcaggaaa aaaatattaa
    14941 gattataaaa gataaaatta ttgacattaa agaagattta ttgcacaata gagattgtgc
    15001 taatttaatc cctaagctag aaaatataga agatgtatgt agggagatga taaaatgata
    15061 tttgaaggat tgtctgataa actacaaggg gcatttagta agttaaaatc taaaggaaaa
    15121 cttacagaag tagatgtaaa aaatgctatg agagaagtta aacttgctct tcttgaggca
    15181 gatgttaact ttaaagtagt taaagatttc ataaaaaaag ttcaagagag atgtgttggg
    15241 caagaagtta tgcaaagctt aactccagct cagcatgtaa tcaaaattgt aaatgaagag
    15301 cttacaagtt taatgggaga tgtacaaagt aaagtaatga tttcctcaaa gcctcctaca
    15361 attttaatga tggttggatt gcaaggtgca ggtaagacta ctacagcagg aaaacttgga
    15421 ggatatttta aaaagcaagg taaaaaacct ctattagtag cttgtgatat ttatagacca
    15481 gcagctataa agcaacttca agttgttgga gaaaaattgg atatacctgt attcaatatg
    15541 ggagataaag aaaatccaat aaatattgcg aaagcaggtt taagccatgc gattaaaaat
    15601 gctaatgatt tagttattat agatacagct ggtagacttc atatagatga agtacttatg
    15661 gatgagttaa agtctattaa atcagaagtt aagccacatg agatactttt ggttgtagat
    15721 tctatgacag gtcaagatgc tgtaaatgtt gcagaaagct tcaatgaggc acttggtgtt
    15781 gatggagtag tattaactaa attggatgga gatactagag gtggtgctgc gctttcaata
    15841 agagcagtta cacaaaagcc tataaaattt atggggatgg gagaaaagct agatgatata
    15901 gaacctttcc acccagatag aatggcatca agaatattag gtatgggaga tgtcttaagt
    15961 ttaatagaaa aagcacaaga aaatatagac ttagagaaag ctaaagaatt agagcaaaaa
    16021 attaaaaaac aagagcttga ttttgaagat ttcttacaac aaatggaaca aatacaaaat
    16081 atgggacctc ttgacaagat acttggtatg atacctggta tgggaaatgt aaaagaccaa
    16141 cttggagatg tagatttgaa tggcaaagaa atgaagagaa caaaagccat aattcagtca
    16201 atgactacat atgagagaag aaacccagga gtattaaatg cttcaagaaa gaaaagaata
    16261 gctaaaggca gtggaactag tgtacaagat gtaaatagac ttataaaaca acttaatgaa
    16321 atgaaaaaaa tgatgaagat gtttgctggt tcacaaaaga gtatgaagaa aaggggcggc
    16381 ttaggcggtt tacctttttt taaataaaat ataaaaaagt aaaaattatt cggaggtgac
    16441 tgtaatggca gttaaaataa gattaaaaag aatgggagct aacaaaaaac ctttctacag
    16501 aatagtagta gctgattcaa gagctccaag agatggaaaa tttatagaag aaataggata
    16561 ctataatcca atttctgaac ctaaacaagt aagaataaat gatgaaaaag ctataaaatg
    16621 gttagctact ggtgctcagc caacagaagt agttaaaaaa ttacttgtaa aaaacggagt
    16681 aatagaaaaa ttcgaagctt ctaaacaagc aaaataattt tttcttggca attagtttaa
    16741 gtaagggctc attgctaagg ttactttagg agatgaaata tatgaaagag ctagttgtag
    16801 atatagctaa ggctctagtt gataatcctg actcagttgt tgttgaggaa tttgaagata
    16861 atgatggtat cgtcttaaag cttacagttg ctcaagagga tatgggtaag gttattggga
    16921 agcagggaag aatagctaaa gctataagaa ctgtcgtaag atctgttgca aatagagaaa
    16981 atataaaagt ttctcttgag atagtataaa ataggaggat taggtataat gcctagtcct
    17041 ttttatataa atgcgtagtt taagatttta tgatgatatg accacataag attaaatttt
    17101 ttaagaaggt gataacatga aagaaaaatt aactcatttt aaggtaggtc aaatagtaaa
    17161 tacacaaggt ttaaaaggtg aagtaagagt ttatccgctt actgatgata tagagaggtt
    17221 tgatgaatta aaagatttct atctgggaaa agatttaaat aataaattgg ctgttgaaaa
    17281 agtaagatac aaaggttcta tggttataat gaagataaaa ggcatagaca gcatagagaa
    17341 agcagaaaaa ctaaaaaata aatttatgta tgtagctaga gaagatagca gagagcttga
    17401 agaaggcgaa ttttttatag cagatatgat tggaatggaa gttttaactg tagatggaaa
    17461 atatgtaggg atactagaag atgttcttca atactcagca aatgatgtct atgtagtaaa
    17521 aggtgaggaa gataaagaat ttatgatacc agctataaaa aaatttgtac ctacaataga
    17581 tattgatgaa agaaaaatga ttatagaccc tattaaggga atgatagatt aggtggtaat
    17641 atgagatttc atataatgac actttttcca gaaatattta attcgtacat ggatgaaagt
    17701 ataatgaaga gagcagttga aaaaggtata attgaggttc atatatataa tataagggat
    17761 ttttctaata ataaacataa aaaagtagat gattatccat ttggaggagg agcggggatg
    17821 gtaatgactc ctcaacctat atatgacaca tataagcata taataacaac tcataatata
    17881 aataacccaa gtgtaattta tcttactcca aaaggtaagg tatacaatca atctatggca
    17941 aagcagatgt ctttaaaaga agatataata ctactatgtg gtcattatga aggaatagat
    18001 gagagaatca ttgatttaat agttactgat gaaatttcaa ttggagatta tgtattaact
    18061 gggggagagc tcccagcact tataatgata gattctatat cgagattaat accaggtgta
    18121 ttaaaccaag aagaatcatt tgaagaagaa tcatttaaag acaacttatt ggaatatcct
    18181 cattacacta gacctagaga ttttgaagga ttaaaagtac cagaagtgct tttatcaggg
    18241 aatcataaga agatagatga gtggagaaga gaagaatcta taaggataac caaagaaaga
    18301 cgatttgatt tatataaaaa atctaatgaa aaataaaaat ataatgaaaa attaatatat
    18361 atgtatacaa taaagtacct ataaatggaa ttaaaaacat aaaagtgctt gtaataacta
    18421 ttgaagtata gtataatata aaatgttgaa aatacgggcg ttcctctgtt tacactggtc
    18481 gggtagttaa gaacgtctat gatatctagg aggaagaaag atgaatgaaa tgctaagagc
    18541 aattgagcaa gaacaattaa aaaatgaagt tcctaatttt ggacctgggg acacagttaa
    18601 agtacacgtt agaattatag aaggaaaaag agaaagaata caagtattcg aaggtgtagt
    18661 attaaagaga caaggtggag gagcaagaga aactttcaca gttagaaaaa tgtctttcaa
    18721 tgttggtgta gaaagaactt tcccagttca ctctccaaaa atagaaaaaa tagaagttac
    18781 tagaaaaggt aaagtaagaa gagctaaact aaactactta agaggtagag taggtaaagc
    18841 tgctaagata aaagaagcta gaaataaata attaaaaact ttacttataa atggggggct
    18901 gatttatcag tcctctttct attttaatga aaattacagc aaataattat actagaaata
    18961 aatagaaata tttgttgaaa atatacatta tttatctatg aatttatagc ttttaaaatg
    19021 aaaaataaaa ttggtaaata ttgcattata ttttgtaaca atattaaaaa taagagaaca
    19081 gtattatgta aagtatgacg ttaaaaagat gacaatatta tagtttgtta agatagattt
    19141 tttaagtgga attaggatat aataatataa tattaataag gagtgataca tgtgtcaaat
    19201 gaatacgaag agtatcttat gaatgacaat ctacatataa actggtatcc aggtcatatg
    19261 aaaaaaacta aggagttagt aaaaaataac ttaaaattga ttgatgtagt aattgaactt
    19321 ttggatgcta gaatcccgtt tagtagtaaa aatccagaca tagacaggct tgtaggagat
    19381 aagccaagag ttgtcgtact taataagagt gatatggcag atagggataa attgaatcaa
    19441 tggattgaat actataagaa aattaatatc aaagctatac cagtagatac aataaaaggc
    19501 gttggtataa ataagataat cgaagaatgt aaaaatgtaa ctagagaaaa aatgagttct
    19561 ttaaaagaca aagggagaaa agaaagagct atacgaataa tgatagttgg agttccaaac
    19621 gttggtaaat cgtctcttat aaacaaatta acaggtagaa aaagcactca aactggagat
    19681 aagccaggag taacgaaagg gaaacaatgg gttagattaa agggaaatct tgaactttta
    19741 gatacacctg gaatactttg gcctaagttt gaagaccaag aagttgcatt aaatttagca
    19801 tttagtagag ctataaagga tgagatttta gatactgaaa ctttggcttt aagacttata
    19861 gagaaactta tgaaaataga acctgagaag ttgaaagcca gatataagtt agattgtcta
    19921 ggagaaactc caattgaaac tatggatatg attggccata aaagagggtt tataacaggt
    19981 aaaaaagaac tagattatac acgcatagcc acaactgtat taaatgaatt tagagatgga
    20041 aaaataggaa atataacatt ggaagttcca gaaaacgtta aaagatagat tttaaaacaa
    20101 aatcaaaaat ttaatgttaa aaatttaaaa aagcctttta gcaaaggctt ttttattttt
    20161 ttgaaatata gacaaatatc tccatataaa agtataaatt aggttagaaa acctttaaaa
    20221 atattaattt aagacaaata tacctaatat tttgaaaatt aaaatgagtg tgattattta
    20281 aaattttagc atattgactt gcttgtttcg tataatatat aataagcaca aataaataca
    20341 atatattaaa aatttaattt atttaatttt attaatttaa taataagttc ttattgttta
    20401 attcaactta cttatgcaag cgtaaacaat cgagacattt tattaattta ataaggttaa
    20461 aaagggggat tttataatgg gtaagaccaa ggatgttata gtatttggat ttgcgttatt
    20521 tgcaatgttc tttggagcag gtaacttaat atttccacct tacttgggaa taattacagg
    20581 tccagaatgg ttaattgcct tcttaggatt tacgtttgct gatgctggat tggcacttct
    20641 agcagtaatg gctacagcta aattcgatgg aaacgtagtt gaaatgttta aaagatgtgg
    20701 tatgaagtta ggaattctta taggttgtgc agatatttta tgtataggac cattcttagc
    20761 tataccaaga acaggtgcaa caacttatga aatgggtata atgcctttgt ttggaacttc
    20821 aattccagtg ttattattct gtatactttt ttttgcgatt tcatatgtac taacaataag
    20881 accttctaag gtcgtagata tagttggaca gtttttaact ccagctcttc taatagcttt
    20941 agcatttata attataaaag gtattatatc acctcttgga gatatagtag ataaacctat
    21001 gatacctaat gtatttgcag aaggtatagg tcaaggatat cagacaatgg atgcttttgc
    21061 agcaatagca cttgcatcag tgctaatagt atctttaaat gataaaggat attctactat
    21121 tagtgacaag ttgaaaatga ttggtaaggc gggtgttctt gcttgtggag gattagctct
    21181 agtttatgga ggattatgtt tccttggggc aactgtatca acaatgtatg gaacagatgc
    21241 agtacaatca caagtaatag taaatataac tgaaggattg cttggaaatg ttgggaaagc
    21301 gattttagca gtagtagtat cattagcatg tttaactaca tctataggtc ttacatctgc
    21361 cacaggtcaa tatttctcta gacttacaaa aggtaaattg agttatgaaa aaatagtatt
    21421 agctgtatct gtattcagtg cagtagtagc atcttttggt gttggaacta taataaagat
    21481 agcatctcct atactaagta tagtttatcc accatcaatt gttttaataa ttttagcttt
    21541 cttcaatgaa aaaattaaga atgataatgt atacaaagga gcagtatata tgtcattgtt
    21601 tgtaagtata ctaacagtta tatctagtta tggagtagct gttccagtag taaattcttt
    21661 acctcttaat tcattaggat ttaactgggt ggtgccagtt ataatagctg gaataattgg
    21721 taattttata ccatcaaaga gtcaatctaa tacacttgga acaaactaag ttttgtttaa
    21781 ttaaaatata atcaattgta aataaatgag
//
LOCUS       DAYAOY010000070        21764 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYAOY010000070 DAYAOY010000000
VERSION     DAYAOY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.070"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            600..1871
                     /gene="serS"
                     /locus_tag="ACGI6B_002677"
     CDS             600..1871
                     /gene="serS"
                     /locus_tag="ACGI6B_002677"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFG9752112.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            2149..3654
                     /locus_tag="ACGI6B_002678"
     CDS             2149..3654
                     /locus_tag="ACGI6B_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9752113.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3768..4466
                     /locus_tag="ACGI6B_002679"
     CDS             3768..4466
                     /locus_tag="ACGI6B_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9752114.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4751..5839
                     /locus_tag="ACGI6B_002680"
     CDS             4751..5839
                     /locus_tag="ACGI6B_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752115.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            6078..7268
                     /locus_tag="ACGI6B_002681"
     CDS             6078..7268
                     /locus_tag="ACGI6B_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9752116.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7359..8861
                     /locus_tag="ACGI6B_002682"
     CDS             7359..8861
                     /locus_tag="ACGI6B_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9752117.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8933..10189
                     /gene="glyA"
                     /locus_tag="ACGI6B_002683"
     CDS             8933..10189
                     /gene="glyA"
                     /locus_tag="ACGI6B_002683"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFG9752118.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10361..13135
                     /locus_tag="ACGI6B_002684"
     CDS             10361..13135
                     /locus_tag="ACGI6B_002684"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9752119.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(13200..13385)
                     /locus_tag="ACGI6B_002685"
     CDS             complement(13200..13385)
                     /locus_tag="ACGI6B_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9752120.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13760..14938
                     /locus_tag="ACGI6B_002686"
     CDS             13760..14938
                     /locus_tag="ACGI6B_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9752121.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      15065..15150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            15268..18186
                     /locus_tag="ACGI6B_002687"
     CDS             15268..18186
                     /locus_tag="ACGI6B_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HFG9752122.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      18372..18457
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18557..19219
                     /gene="zmp1"
                     /locus_tag="ACGI6B_002688"
     CDS             18557..19219
                     /gene="zmp1"
                     /locus_tag="ACGI6B_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HFG9752123.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19341..20303)
                     /locus_tag="ACGI6B_002689"
     CDS             complement(19341..20303)
                     /locus_tag="ACGI6B_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9752124.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20473..21663)
                     /locus_tag="ACGI6B_002690"
     CDS             complement(20473..21663)
                     /locus_tag="ACGI6B_002690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9752125.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8602 a         2513 c         3702 g         6947 t
ORIGIN      
        1 attaattgaa cttattagtt tattaaaaaa ctatgtatat tactttgcag taagactttg
       61 gaactaaatc tataatagca aatttaaatt tattaaatag attaccttat aacatttcat
      121 ataaaatata ttgtgaatct gagaaaatta ctatttttct ttgagtattt tttgtaagca
      181 gttctaaaag attcaatttc atacctatta cttgaatcat tcattaaaat aattctaatt
      241 taaaaaataa ttctatccaa ataatagata tataggacta tgttataata taaaaaaatt
      301 tatataaaag ttaataacta tacaagattg ataaagaaaa agtcaaaata gataattgat
      361 tagcaaaata gatataatta taatgaagtt aaatctagca atattctcct aaaattaaat
      421 tacatttatt taattaaata taatttgaca atggacaaaa aagtgagtaa aatataaata
      481 aaataaaaat aacaggttcg gataaagaaa aaggtttctt aacctcaagc ataaagtata
      541 ttatctaagt tatttgagat taagagataa atccaatctt aggtactgag gaggaaaaaa
      601 tgcttgatat aaaaagaata agagaaaatt tagattgtat aaaaaaagca atggaaagaa
      661 ggggcgaaaa agactttaac cttgatgaag tagttaagtt agatgatgaa agaagaaaga
      721 ttcttcaaga agttgaagtt atgaaaaatg aattaaatac agcatctaaa aatataccaa
      781 atcttataaa agaaggtaaa gatgtagaaa atgaaaagat taaactaaaa gagttatctg
      841 ataaaattaa ggtaatagac caaaatctta aagaagtaga agataagatg gaatacctat
      901 taatgagaat accaaatgta ccacatccag aagtaccaca aggtgaaact gatgaagata
      961 atgttgaagt aagaacttgg ggaaagacta ctacctttga ttttgaatct aaggctcatt
     1021 gggaaattgg aacagaatta ggtatacttg attttgaaac agctagtaaa ataactggtt
     1081 caagatttac tctttacaaa ggattaggtg ctagattaga gagagcatta ttaaattttt
     1141 acttagacac gaatacaaaa gtaaatggat atacagaagt tattccacca tttatggcaa
     1201 atagaaatag ttttttaggt acaggacagt tacctaagtt tgaagaagat atgtttaaaa
     1261 tagaaggtat ggactatttt atgattccaa catcagaagt tcctttaact aatatacatg
     1321 ctaatgaaat acttaaattt gaacaattac cgataaatta tacagcttat actccatgtt
     1381 ttaggtcaga agctggttct gctggaagag atacaagagg tttagtaaga caacatcagt
     1441 ttaacaaagt agaaatggtt aaaatagttg caccagaaga atcttacaat gagttagaaa
     1501 agcttactaa caatgcggaa actatgctac aattattgaa tctgccttat agggtagtaa
     1561 agatatgtac aggtgattta ggatttacgg cttcttttaa atatgatgta gaagtttgga
     1621 tgccaagtta caataggtat gttgagattt catcttgttc aaactgcgaa gatttccaag
     1681 ctagaagagc aggaataaga tttaaaagag ataaagattc taaagctgaa tatgtacaca
     1741 cattaaatgg ctctgggtta gctattggaa gaagtgttgc agctatatta gaaaattatc
     1801 aacaagaaga tggttctgtg gttgttcctg aagttttaag accatatatg ggagtttctg
     1861 ttattaagta agagttaaat tatgtatttt taagcaatac aataatatag aggtaataaa
     1921 aaataattaa tataaaagtg atacaataat atagaagtaa taaaaaaata gttaatataa
     1981 aagtgataca ataatataga agtaataaaa aaaaataatt aatataaaaa tgtgtacaaa
     2041 ctaatgaata ttgatatagt aagctatcat atgaaagagt atgtacacat tttttttact
     2101 aatttttcac aatatgaaca taaataattt acaggaaagg aatcttttat gaaaattcaa
     2161 aatactgagt ggggatatat tgaatggaaa catacttatg atgaaaataa tccaaaacaa
     2221 gctatgaata tatacattgc agtaactatg cctggtaaga aacattttaa tcatgttcat
     2281 tatggtcaag agcagatgat atatatttta gagggagagg gtttgtatat tataaatgga
     2341 gtttggaagc cattttatca aggtatgatt ttttatatag aatctggtag cacacatgaa
     2401 actataaata caggtgatag agaaataaaa gaacttattg tttcaaataa tgtagatgat
     2461 gtaggagaga gtgaagttat agatattaat cctaataatt atttaaagaa gaccttgata
     2521 aattatagtg aaagtacatt aaatttgtat gcagcagtag aatcaattcg tggtcaattt
     2581 atagacccgt ttaaaattcc attaattata tatgatgatt catggaatat agttttaaaa
     2641 aatccttatt ttccactttt ttgttttgaa aaatgtaatc ctatgaaatt tcctcaaaat
     2701 tgtgattgta tgaatcaaaa aagtagcaat caatttgtat gtgaatatgg gataactata
     2761 tataatatac caatactgta caaaagcaat agcataggtg ttataagagg agggtatgta
     2821 ctattatctg atttgaattt agatacagag cataataatt tatatgatat tccagaggga
     2881 gctgctagaa gtataaaacg cctcttgaaa caaatatcta aaaatataat aaatttttgc
     2941 tcttttaatg atataagaaa ggatttacaa gaaaaagaga agactatagc aagaacatat
     3001 cattatggag agcaacttga aatgaattta aaagtagctc aagatatggt tactaattta
     3061 agaataaatc atcacttttt atttaataca ttaaattcaa tggcaagcat agcactagat
     3121 gatggtagtt atgatttgta tagtgcaata atagatttat caagaatgtt tagatataca
     3181 atgaggtctg atttaagatt tgtagaatta gaatctgaaa tattatacat taaaaattat
     3241 ttgaatttac agaaattacg ttatggagat gcacttaaag taaaatatct tattccagag
     3301 aagttatata acctttctgt gccttttaac tttattcaac ctattgtaga gaatgcattt
     3361 acacatggat ttagagatat tgacacagaa aagcgaattg aaataattgc aagattagat
     3421 tctcagtatg ctattataga gattcataat aatggaacta tcttagatgg aaataatata
     3481 gacaaaataa aggcaggtat tcgtagtaat aatggtcatg gtttgtcttt aatatataca
     3541 aaattcacat ctgcttatgg aaataatttt gatatggata taaagtcgag tgataatgaa
     3601 ggaacttaca taatgattaa aattcctatt gaaaattata aggagtatgt atagaacttg
     3661 tgaaaaatta ataagtttta taaatgttaa gaaatatatt agatgttttt atgttgtttt
     3721 atatatctta cttttttata ttattaagct aaaagggagg tattctcatg agaagagcac
     3781 taataataga tgatgaagaa gctgttctaa aaataataac acattttata gaaatgaaag
     3841 atatgccaat aaaaattgtt ggtaaagcaa catctggtga tgaagcagta gataaaatca
     3901 ttggtttaga gccagacata gtttttatag atattcaaat gccaatatac aacgggttag
     3961 aagtaataga aaaaacatct catttatcta aaaaaataaa ctttgtagta ataactgcat
     4021 ttaactattt tgaatatgct caaaaagctt tgagattaaa tgtaaaagat atactcctta
     4081 aaccattgga tatgaaagaa tttactaagt ctgtagaaaa aattattgga tatcaatata
     4141 ccaataatga tttattaaat gagatactgg aatatataaa tctaaactat agcaatgact
     4201 taaagttaaa tgattgtgct agattatttt taaccaatcc atctaatata tctaggattt
     4261 ttaaaagtaa tttaaataca acttttatat catatctaaa tcatataaga atagaaaaat
     4321 ctattgagtt acttgaaaat actacaaaaa gtataaatga gattgcagaa atagttggat
     4381 ataatagcct aaataacttt tataaaaact ttaagataga aaaaggaatg actccaaaag
     4441 tttataaact gaacactaaa aattgaaatt gataaataaa aagtcaatat agataattgt
     4501 ttgagtgtgt ctgaataagt atactttaat tatgataaaa attaaagcgc tttaaacata
     4561 tttaataata ttaaacctta taaaagctgt acctatttat ataaaaaaat atatataaat
     4621 aaaaagaaaa agatatacaa aataaaaaaa gtgaattaaa tactgtttca aaaagacaga
     4681 attttcattt aatttactga gagtttatat ttcttaaagg aaggtaactt tataatggag
     4741 gtaatttaat atgtcagaca aagtgaatat gaaaaatatt atctgtattg caggcgctta
     4801 tctagcaaca gcaattggtt ctgggtttgc tactggtcaa gaaattctac aattttttgc
     4861 ctcacaaggg tatatgggtt taattggagc attaatatca atggtattgt tttcatttct
     4921 tggtgctgaa gttataacta gaggtagaga attaaaactt aaagaaccaa caagaattta
     4981 cactttttat tgtgggaaga ttttaggaaa attttacgaa tggtttagtc caatattttt
     5041 atttgggatt cttatagtaa tgatttcagg tgcaggctca actttaactg aatactatgg
     5101 tataaatcct tacttaggaa gagtattgat ggctgttgct actttaatta cagtgtctat
     5161 gggtttagaa aaattagtaa gcatacttgg acgtattgga ccagtgatag cagtatttac
     5221 aattgctgtt ggattaataa gcttgtttaa gcattttgat ggtttatcta tggttggaga
     5281 agctatgaaa aatattgaag ttacaaaagc atcagaaagt ccaatcgttt ctggtgtaat
     5341 atataataca ttaaatatta ttgtaatgat tgttttttta acaggaatgg gagcttctat
     5401 tgaaagtaaa aaggatgctt tttggggagg tttattaggt ggaatagtgt ttatgactgc
     5461 tggtatagtt atgtatttgg ctatgatttc agatatagga aatttgtata tgaaagaaat
     5521 accatcactt tatttagcag ataatatatc tcctattgta ggtgtatgtt tttcagttgc
     5581 attaatatta ggtatctaca caacagctgt tccactttta tggtctgtaa ccaatagaat
     5641 tgttgaagat gaacatccta aatttaaatt ggtaacaaca gtaatagcta ttttggcttg
     5701 tataggaggt tttttaccgt ttggtaaact agtaaacatt ttatatccat atacaggtta
     5761 tatggggctt ttaattttag tctgtatggt gtataaagca attacatgta gaaaaaaatc
     5821 aacacaaagt caggaataga gccacttatt aatagttttt atttaaagaa attttttata
     5881 taatccgaat ttaaaaagtc aaaatagtta aataaattgt aaaatatgat aatatattta
     5941 attaaaataa tatagtaaaa tataaatata aactaaaatt aaatttataa aaaatatctt
     6001 tataaagatt attaacttat ataatttgta gttaaattat aaaaaataaa aaaataaaat
     6061 tttggagagg tgttagtatg catgaactta ttttaatctc agaaaagtat aaagaagata
     6121 ttttagattt ttttacagat attcatatgc accccgaatt aagttttaaa gaatttagga
     6181 ctacaaaagc tataaaagat ttattagtga gtttagacat agaaatatta gatttaggaa
     6241 tggaaacagg agttgttgga ttattaaaag ggaagtatga tggtcccaca gtagcactaa
     6301 gaggagatat tgatgcattg ccaatatatg aagaggttga tattaagtat aaatccagat
     6361 atgatggtat aatgcatgca tgtgggcatg atatacacac gtcttgcctt gtaggatgtg
     6421 catatgtatt gtctcatatt agagatagtc ttcatggaaa tgtaaagttt atatttcaac
     6481 cagccgaaga agtcaataaa ggtgcgaaga tgctagtgga acgaggagta atggaaaatc
     6541 caaaagttga tgctatattt ggacttcata atcatccaga tataccttgt ggaaaaatag
     6601 gtgttaagtt aggtgggtta atggcagcag tagacactat taagatagaa gtaaatggat
     6661 ttggaggtca cggaggtata cccaacagaa caatagaccc tatagtagca tcaagtgcaa
     6721 ttataatggg tattcaaacc atagtaagca gaaacataag tccattagaa tcagctgtta
     6781 tatcaatagg tactataaat ggagggactg caaataatgt aatttcagaa aaggtagata
     6841 tgacaggcac atgtagaagc tttagtaatg aagtacgaaa gaaaatatca gaaaatttag
     6901 aaaatatagt ttgtgagata gctcgtggat atcaagcaac tgcaaagcta gattatttat
     6961 ttgatttacc agctgttata aatagtaaag agatgtacgc aatagcttgt aagtctgtgt
     7021 gtgatttgta ttcagaagat gctatagttg atccaatacc ttctacagga ggggaagatt
     7081 tttctatatt catggaaaaa gccccaggat ttttttactg gttaggggta ggcaacaaag
     7141 aacaagattg tatatatcag tggcataatc ctaaatttaa agctgataaa aattctatat
     7201 tggtaggtac aaatgtacta tgtcagtctg ttataaatta tatggataaa ttaaaaaata
     7261 aaatttaaga tagagcattc tggagataaa gtttatgagg gggtattagt ttgaggaaaa
     7321 ttaattcaca ggaaaaaaac aaagatagtg gattattgat gagggcacta aatgttgtag
     7381 agagagcagg aaataaactt ccagacccag taaccatatt tttattacta tgtataattg
     7441 ttgtaatatt gtcagcagtt atatctaatt taggagtaga agaaatacat ccttctacaa
     7501 aagaagttgt aaaggtagta aatctactag agaaagagca gattcaatca tatttaggta
     7561 gcatagttac aaactttcag tcatttgctc ctttaggatt agtactggtt acaatgcttg
     7621 gagcaggtgt tgctgagaaa tctggattta tggaagtgtt aatgaaaaag ggtataagta
     7681 aagttccaca aaaacttgta actgtagcaa tcgtatttgc aggtatgttg tcacatacag
     7741 cagcagatgt aggatttata atattaccac cacttgcagc acttgtgttt ttaggaattg
     7801 gaagacatcc tcttgtaggt atgtttgcag cttttgcagg tgttgcaggg ggatttgcag
     7861 caaatgttat gcttagtact acagatgtat tgttagctgg atttacaata ccagcagctc
     7921 aaatgatgga cccatcttac caaggcaatc cagcaatgaa tctttatttt gcttttatat
     7981 cagcgatagt tttaactttt gcaggagcat ttgtaactga aaaatttata gctcctcgtt
     8041 ttacaaaata taatgattca acaaatgata aggatataca ggaattgagt gaacttgaaa
     8101 ataagggtat aaaatacgct ttattatcat tgttagttgt agtagtagtg atagttgcct
     8161 tatgtatagg tgacaatgca tttatgaaag atccagagac tggttctata ctatctagta
     8221 atgcaccttt aatgaaaggt atagttccta taatcactat tatattttta actccaggat
     8281 tagtgtatgg aaaagtaagt aagaagataa aaagtgacaa agatttagta tctatgatgg
     8341 gtagtagtat gagtgatatg ggtggatata tagtcctagc atttatagca tcccagttta
     8401 ttaatttatt taatcttagt aacttaggaa caattttatc aataacagga gcaaaattac
     8461 tagcagagtc gggaatacct agctatggat tgataatagg atttatattg ctatctggat
     8521 ttatcaattt atttgtagga agtgcttctg caaaatgggc gattttagca cctatatttg
     8581 ttcccatgtt tatgctacta gactttaatc cagcattaac tcaaatagct tatcgtattg
     8641 gagatgcttc aacaaatcca ataagtccac tttttccgta ttttccagtt atattggcct
     8701 ttgccagaag atatgataaa gatataggta taggtactgt catatcgaat atgattccat
     8761 actcagtggt atttacatta atagaaataa taatattgtt actattcatg ggtattggca
     8821 taccattagg acctggtgga ggaatatctt acgttttata gaccgttata ataaatttat
     8881 caatgaagat taaactaata aaatatattc tttaattaag aggaggatta ttatgttaaa
     8941 aacaacaaaa atttcagacc cagagttata caagattgta gctgatgagt tggttagaca
     9001 agaacacaat attgagatga tagcttctga aagtacagca ccaacagaag tacttgaatt
     9061 aagtggatgt gtatttacaa ataaaacaga agaaggctat ccaggggctc gttttcaagc
     9121 aggttctgaa gaagcagata aacttgaaac cctagcaata aaacgtgcaa aagaagtatt
     9181 tggagcagaa catgtaaacg tacaaccgta ttcaggttct acagctaact attgtgtata
     9241 ttcatcaata ttaaaaccaa atgacactgt tttaagtatg cgtcttgacc aaggtggaca
     9301 cttgacacat ggtagcgcag ttaatttttt acatgacatt tataaatatg aattttatgg
     9361 ggtagaccca aatactggac gtatagatta tgatgctctt gaagcaaagg caaaagaatg
     9421 tagaccaaaa cttataattg caggggcaag ttcatatcca agattaatag attatgaaag
     9481 aatatcaaag gttgcaaaag aagtaggagc ttactttatg gtagatatgg cacatgttgc
     9541 aggattagtc gcagcgaaag taatacctag tcctgtacct tatgcagatt ttgtttcttc
     9601 atctacgaca aagactttct gtggaccaag aagtggtatt gtactttgta aagcagaaca
     9661 tgcaaagaaa ctcgataaag gtgtttttcc aggaacatta ggttctatac atcttaatac
     9721 agttgcagca aaagcctttt cactattata tttaagtaca gataaattta aaaagataat
     9781 ggagcaagtt gtagtaaatg ctcagactct agcttctgaa ctgatttcac atggttttag
     9841 tattgtaagt ggaggtactg ataatcatat agtaatggtt gacttaagaa gtaagaatct
     9901 tacaggaaaa caatttgaaa aagctcttga atatgtagga ataacagtta ataaaaatgt
     9961 tatacctgac gacccacaat caccatttgt aactagtggt gttcgtatag gtcttacatc
    10021 aatatctcaa agaggtctga aagaaaaaga agttatacag atagcaggga taatgaataa
    10081 ggtagcagaa aatattgata ataaagaagt attagatgaa tgtaaagcag aggcacaaga
    10141 acttatatct aagtttccat tatatccaga agggtatttt gaagattaaa tattttgatt
    10201 tgtatagctc acaatttata aagcctgtaa cataaaattt aataatatat aaccactata
    10261 aggtgttgat taatcaacac cttatttttt ttgaaaagta atatacaagc tatcataagc
    10321 atataattcc ttatagataa ttaacaaggg ggataaagtt atgaggtatt ataataaacc
    10381 taccaaagaa gtattaaaat acttaaaaac aaatccagaa attggacttg atgataatga
    10441 ggtagaagaa aggaagctta gatatggatt aaatgaattt actataaaag aaggtagaac
    10501 attttgggac gagttgggag aaagtttgac agaacctatg atattaattc tcataggagc
    10561 agcagtaata agttcttttg tgggagaatt acatgacgca ttaggtattt taggggctat
    10621 ttttattgga atttctatag gcataataac tgaaggaaaa tctaaaaaag cagctcatgc
    10681 cctgtcaaaa ttaacagaaa atatagaagt aaaggtgctt agaaatggga agataattaa
    10741 aatatcaaaa aatgatttag taccaggaga tattgtatat atagaaactg gggatatgat
    10801 accagcagat ggaagactta tacagtctat aaatttgaaa cttagagaag atatgctaac
    10861 aggagaatct gatgatgtag ctaagaatgc tgatgctgta ttagatatgg aagtagttta
    10921 ttctaaaaca gaaataatcg aacaggatgc aatacctgca aagcaagtca atatggtatt
    10981 tggagggact cttgtagcat atggtagggg cataatggtt gtcactcata ctggtgataa
    11041 gacagagatg ggtaagatag cacagaattt atcaaatgaa gaccagcaaa ctccacttca
    11101 aattaaatta gggaagttgg gtgctaaaat agcaggaata tcaggcataa tagctacttt
    11161 attgtgtatg tttatgatta tacagatgca gagaaaaggt atgttaattt tagatacaag
    11221 cagtatatta tctttcttac aatcattaga acctgctaaa aatgcatata tggtttgtat
    11281 tgcattaata gttgcaactg ttccagaagg tttgccaact atgataaata ttaccttagc
    11341 tataacaatg caaaagatgg caaagataaa tgcattagta acaaagaaag aagcctgtga
    11401 aactataggt tctgtttctg taatatgttc cgacaaaaca gggactttaa cacaaaacaa
    11461 aatgatggta gaggtagcat atgttgatgg taaatatata agtggaggag agtatcaaag
    11521 taatagctat tttgaacaaa actgtatagt aaattcaact gcagatatag aaaaagaaga
    11581 taattcattt aaatatatag gaagcgcaac agagtgtgca ttacttttat atcataatga
    11641 taagaactat aatgaaatga gaaaacaaac gtatttgata tcacaaatac catttagttc
    11701 agaagaaaag aagatgtcaa cattaattcg tcaagaggat tcagatatat tactttcaaa
    11761 aggtgctcca gaagtattat taaaaaaatg ttcttatgta caacaaggaa aaaatattgt
    11821 tccaataact ccaaaagtag aaaaaagtat tttagatgag attaagaagt tacaaattaa
    11881 atctatgaga acattaggat ttgcttataa aaaaatgagt aattctaaaa cagaagttgc
    11941 aatgacttca gaaggtgaat tgaatttaat aggaaattca agaagttata tgaaggaaga
    12001 taatttagtt tttagtggat ttgtaggaat agtggaccca ttgagagaag gggtcaaaga
    12061 ttccattgat aaagcattta atgcaggagt agatgtaaaa atgcttacag gagataatat
    12121 aaatacagca acagctattg gaaatgaatt aggattatta aatgatggga aaaaggcagt
    12181 tgaagcaaca tatatagatg tattgacaga taaagaattg agagaagaaa tcaaaggaat
    12241 atctattgtt gcaagaagta aaccagatac aaaaatgaga atagtttcag cacttcaaaa
    12301 aagtggcgaa gttgtagctg taacaggtga tggtataaat gatgccccag cacttagtca
    12361 agcagatgtt ggaattgcaa tgggtatttc aggaacagaa gtatctaaaa atgcagcaga
    12421 tataatattg actgatgata gctttagtac tatagtagag ggaataaaat ggggaagagg
    12481 tatatatgag aactttcaaa gatttataca gtttcagctt actgtaaata tagtagcatt
    12541 tataattgct ataatatctc aacttactgg taaggatatg ccatttacta caatacaatt
    12601 attatgggta aatataataa tggatggtcc tccagcccta gcattaggat tagaacctgt
    12661 tagagattat gtattgaaaa gaaaaccaat aaatagacat tctggaataa tagctaggtc
    12721 tatgtttgta aatattatta taaatgcaat tcttataatt acaattgtat ttactcaatc
    12781 agcttttaat atattgggag ctacatcaga agaacaaggt actgttatat tttctttatt
    12841 tgcattcagt gcccttttta atgcattaaa ttgtagagag tttggtttaa atagtacaat
    12901 accaaatttc tttaaaaata aactggcatt gcaaataata gtagttacag ggataattca
    12961 aataatattt acacaagtgt tccagagttt ctttaattct gtttctttgg actttgatat
    13021 gtggataaag ataatactat ttgcatccac tatcctattg tcaaatgaat ttgtaaagct
    13081 aattcttaga acaatgaaaa atagtagaac tatgaacttt aatagtaaaa attaattctt
    13141 atgaaaccgt aaagtactat atattgtaac tttgcggttt ttgtttgttt aatgttgttt
    13201 tatattacca ttttcatatt taatttttta ttaatctgaa cacaatcttc cattgtaatt
    13261 gcttcatctg tgtcaacaca ttcaaagtca atatctaatt tttgtaatat tttatatgcc
    13321 tctttttgca aaggagtatc aaatttaata ggtgattttg tgtaaatatc actgataaaa
    13381 aacatgattt atttcattcc tttccattta aaatcttata gttatagtgt atcataaggt
    13441 tataaaatat agataaatat agtataaatt taaaatatgt acagacaaaa caaaaaaatc
    13501 atattctaag taatacttgg gaaaaatgat attatcataa taaataatct ttgtaaaaac
    13561 ttatatagaa tctattaaac agacactaag ccataaaaac tttactaaat ataaaattcg
    13621 tatctaaata gatatataga ataatatttt actgaagtag agtgattttt aagaatttta
    13681 tatataaatt atgttatcat aaataaagat atagattata aaatttgtta ataaaataaa
    13741 gggcagggag gtaagtttca tgttttccaa aatcctagtc aaaacattta tcagagatag
    13801 cgaaaacgtt caaaatacag atgttagaaa taagtatggt tatgtagctg gtgtagtagg
    13861 tatattatcc aatttacttt tatttgtgat aaaggtattc ataggaatgc tcacatctag
    13921 tatagctata atggctgatg catttaataa tctttctgat atggcatctt cagcaataac
    13981 tatgataggt tttaaattag caagtaagcc agctgataaa gaacaccctt ttggtcatgg
    14041 aagaatagag tatttatcag cattaatagt agcatttatg gtgatgttgg ttggacttca
    14101 atttgtaaag tcatctatag aaaggatagt aaatccaatt cctgtaaaat ttgaagtaat
    14161 accacttata cttttaattg catctataat gattaaaatc tggttgagtc gttttaataa
    14221 atttatggga aataaaattg attcttcagc cttaaaagca gtttcacttg atgctttagg
    14281 agatgtattt acttcaagtt gtgtagttat atcatttatt gtagctagat ttactaattt
    14341 tcccatagat ggatatgttg gtatcgtagt atcattagtg atactttatg ctggtttctc
    14401 tcttgtaaaa gatactataa atcctctttt aggggaagca ccggatgaag aaatggtaaa
    14461 ttctattata gagctattgt tatcttataa atatataata ggtacacatg atttaattat
    14521 tcataactat ggagttggta gatgtattgc atctatacat gcagaaattc catccaacat
    14581 agatataatg gaaatacatg aaattataga tactgcagag agagaaatat ctgaaaaatt
    14641 agatatatat ctagtaattc acatggaccc aatatgtctt gaagataaag aagttatgtc
    14701 agccaaaaaa gaacttgaag aaatacttaa gaaaaatagt cttgtaaaat ctatgcatga
    14761 ttttagaata gttggaaaag gaagcaagaa gaatttaata tttgatatag tagttaatcc
    14821 ttccgaattt tccaaaaaca tgtcagaaga tgacttaaaa gaagatataa caaaattagt
    14881 taaggagata aatcctgaat ataattgtgt aattgttgta gataaggatt ttatttaatt
    14941 attaattaat ctcatcataa ggtaaaaaat gtaaaaagga tagacaatag tatagaggca
    15001 taatataata gaagtatata attgtatttt taatataaat aaagcaattt taattgtttt
    15061 tatgaaatat tatagagatg ttgaagtata ttctattatt gggcacctta tggatatact
    15121 gagtcagtgg tgcaaccggc tatgaatata aatttattta ttttcatagc tttttttgta
    15181 taatttttta gtaaaaatta ctttatatac gatattaatg gaagattaga ttataaatta
    15241 taggtaacct aaaggggtga gatttacatg aagaaaggaa atagaaaggc attattaatt
    15301 agtttaatta tgattttaag tatggtggtg tctacaatat atccaactgt atcttatgct
    15361 tcggaattag gagagaatag tcagattcaa agtggttcaa ctaattcatc aactggagag
    15421 gagaaggaaa gcgacaataa aaagccagaa cagactccag agaaagataa ggcaacagat
    15481 aataaaaaac cagaacagac cccagaggaa gaaaaaccaa cagataataa aaaaccagaa
    15541 cagaccccag aagaagataa gtcaacagat aataaaaagc cagagcaaac tccagaagga
    15601 gaaaagccaa tagataataa aaagccagag cagataccag aagaagataa gtcaacagat
    15661 aataaaaagt cagaacaagc tttagaagat gaaaaaccac tagacaataa aaatacagaa
    15721 aaaactccag aggaagataa tctattagaa aatgaaaatt tattgaaagt tttagaagaa
    15781 gaacttaatg aagaaaatga agactatgga tttgttgtaa aaattaataa caatacaata
    15841 gaaacggaat caatgaaaaa aatatcattt aatttaactt atactccaac atcaaaagga
    15901 atacaagcag gagattcaat aacttttaag gtgccagatg tattcaataa agttaattta
    15961 gattatacaa gtgaatgctt tgataaaaca gaaagtaatg gagagtatac acttacattt
    16021 agagaattac ctaatggcca aagtgtaatg caaggtaaaa taggtttgga agcatatgtt
    16081 aaaaaggttg atgaagatac taatgcaaaa atacatattg aaactactgg aaaaatagaa
    16141 agtggaagtg gagatataga tgtagaaata aaacctggag ataaaacgga tgttccagat
    16201 gctaaaggaa cacttaaaaa gcttgttgaa ggaaaaaaat caactactgt atttatgcca
    16261 gttaaaaata aagatataaa ttacagtata caagtaaatg aaaagcagga agagttaaaa
    16321 gatattattt tgtatgatga gcttcctgaa ggattgacat tgataaatgg ttctgtaagt
    16381 gtagtaacaa gtgatggaaa agaagttagt gattttaata ttgaacagag taaaaattct
    16441 atttcagtta atcttggaaa tatagataaa tcgtatactg ttaagtataa ggctagaatc
    16501 agtgacaaaa atgcaaaaca tggtaataaa tataagaatg tagctaggat tgaatctgat
    16561 ggtaagaaga tacaagaaga tgatgctact gtaagtatat ttgatagagg agatgattat
    16621 cttcttacta aaggacattc aggagcaaca aatattactc aagttggtca agtaataaac
    16681 tatcagattt caataaatga tgataaatca ccaataagta atgttgttat aacagataat
    16741 ataccagaag gcatgagatt aactacttca ggagaagctg ggcatgattt tagagttgta
    16801 gaaattccaa tgaatgggag ctggactcct tggagtaaag aaaaaattgc caataatatt
    16861 tcatataaag tagaagaaaa aagaaatgaa agtgggcaag tagataaagt tataactgga
    16921 tttacaatta atctcagtaa agaagaagtg gaatcaaagt tttttatagc ttatacatta
    16981 aaggtaatta gtattgaaga tagctatata aatagagctg ttcttgatgc aaataatagt
    17041 gaaattgata aaaatgatga gataaacttt aagaaaaatt ctggattaat aagcgcaaaa
    17101 aaagaagttg ataaaaaagt tttaaatagt agtgataacc agattgttaa atacaaaatc
    17161 aatatgtcaa cttatggggt ttatgatgca ggacaagtaa atcttttaga tgaagtaaac
    17221 tcagctctag aaatatctaa tataaaatat tctgataatc ttgaacttaa aaaagaagct
    17281 ggagatggaa aaaatacaat aagattagta aataaatacg aatttaaaca gataaaagaa
    17341 ggggaacctg tgcaaagttg ggttacattt gatgcagact tcacaaatgt aaaagttggg
    17401 gagactatac gaaatgtggc tcagattaat ggttcaagtc ctccaggagt agagactact
    17461 aagcaaggtt atgcttttga agctaaaaaa gtggatgctt tagataaaaa tgtactttca
    17521 ggtgctaagt tcaatttaga agatgcattt ggaaatatag tagttaagga tttagttagt
    17581 gatgaagatg gtataattca atctagtata aaaaaaccag gaacatatta tttagtggag
    17641 ataatggctc caagtggata tgaaaaactg aaggataaaa ttaaggttga aattggtaat
    17701 aaagatattg gtagaatagt ggatattgga aatatagaaa acataagaca agaaaatcca
    17761 ccagtaaatc caccagcacc accaaataca gatgaaccaa tagtaaatcc accagtacca
    17821 ccaagcacag ataaaccaag gaaaccatca agttcatctg atacagagga tactatagtt
    17881 ataaatccac caataccacc atcagaagat ataataaatc cgccaatacc agaggtattg
    17941 aatccgccag taccaccatc agaagagatg atagaaactc ctgttaaaca gattattcca
    18001 ataccagaag tagtaaagcc atcagtgtca gaagaaaaaa ataataaagt taaagatgat
    18061 actttggtaa atccaccagt accaccaaaa acaggggata gtactacaat tattggagaa
    18121 atacttttgg ttataggagc tattgtagga cttatcgtat taagaagaaa taaaaataca
    18181 aattagaccc taacattata aaaaatataa gttatattaa aaaatatctt ctaaaacaat
    18241 atttaataat gaaaatattg ttagaagata tttttttttg aagatttatg taaaaaaata
    18301 ataacttttt agaaaaaagt tccaaaggga tatagacaat taaaaagata taagctataa
    18361 tatagatata aaattaaatg aggcaaaact agggaaacct agtcacgcaa agctataggg
    18421 gctaaagttt agtaaaccat gccagccagt tgccgaaaag agtaaaaatt tctttttgta
    18481 tatacaaaga tactcttggg tatctttttt attttgaaat ttattactcc cggcctaact
    18541 caaaggaggg aataatatga gaccaagtaa aaaattatta atagctataa tatcaatatt
    18601 cttaataagt tcagttccag taagtgcaca tgcagatagt actactatac aacaaaataa
    18661 agacacactt agtcaaatag tagtttttcc aactggaaat tacgataaaa acgaagctaa
    18721 tgctatggta aatagattag ctaatataga tggaaagtat ttaaatgcat taaagcaaaa
    18781 taacttaaaa ataaaattat taagtggaaa gttaacagat gaaaaagaat atgcttattt
    18841 aaaaggtgta gttcctaagg gatgggaagg tactggaaaa acttgggatg atgttccagg
    18901 tttaggtgga agtacagtag ctcttagaat aggatttagt aacaaaggta aagggcatga
    18961 tgcaataaat ttagaattac atgaaacagc acatgcaata gaccatatag tattaaatga
    19021 tatatctaaa tcagcacaat tcaaacaaat atttgctaaa gaaggtcgtt ctttaggtaa
    19081 tgtaaactac ttaggtgtat atccagaaga attttttgca gaatcatttg catattacta
    19141 tttaaatcaa gatacaaata gtaagttaaa atcagcttgt ccacaaactt atagcttttt
    19201 gcaaaattta gctaaataga gttttaataa atttaaaata aatataaata aagagatgct
    19261 attataatag catctctttt atattgcgtt taaatctaat aaattttgtc ttatgtataa
    19321 ctatgataga ttatcaatta ctactcctta tacaaggctt caacgtgttt attatgcatt
    19381 tgagtaatta tcgtaggtat aatagaaatt attgaaccaa ttactatcaa taaaattcct
    19441 ataaccatgt ttatagggat aatttcttta agtattaata atgctagtat tggagatatt
    19501 actggtttaa agaagaatac taaagatgta agactagtag atgtagcttc cattgccata
    19561 aagtaaagcg cataaccaac acctgtaaca aatacgaata cataaatcat aatgggcata
    19621 ttgtgtaagg tatatccact aaaaataggg atatttgcaa atacatgaag cttattttta
    19681 tttaatatat ctgcgataaa attaatttta gtgagtaaaa ccaatatcaa catttccaaa
    19741 ctaccaaata taaagctaaa gcaggttaca actattccac caaattttaa ggtttttctt
    19801 ttaccaaata cagtatacat tgaaaaaata atagcagaag ataatgttaa gaatattcca
    19861 ctaagagtta gttttgtatg caatggattt attataacta taattccaag aacttctaat
    19921 ataagtgaaa ttacattgtg cctatatatt ttttccttca aaattaagta agctaaaagc
    19981 attacaaaga ctggattaca actgaacaaa acagcaacta cagatgcctt tgtgtttaga
    20041 acagcaagtt gatacaatga catacttact actacacaca taaaacctaa aaatgcaaag
    20101 tattttaagt ctgattttgt aatagaaaga ctaagagctc ttaatctttt tacagcaaaa
    20161 ggtagtaaga ctaaaccgcc aacaaaaaat cttgataaag ttatttgtaa gggattaaag
    20221 tcattagtga cagtttttag actaatttcc attgaactaa aaaataaggt agtcagtatt
    20281 atataaacat aacctttttt caagtcaaac acctccttgt aatttatatt agtactatag
    20341 ataagaatat tcaagcatat tggaataata tggtatgtat aaaatatagt acataaaatt
    20401 ataaactacc ttaatataga atgtaagtat tctatactaa ggtagttaat tatttgaatt
    20461 actttgattt agttatttta aattatataa tttggctaat ttttcaaatg ctggtaaaga
    20521 acttttaact ttatcataag atacacaata tgatactcta aaatgacctg gacaaccaaa
    20581 agaagaacct ggtactaata ataaattaaa ttgttttgca tcattgcaga attttttatc
    20641 atcttcaatt ggagcttttg ggaacaaata gaatgtacca ttaggtttaa cacaagaata
    20701 tcccatatcg attaaactat tatatagaag gtcaaaattc tttttataaa tatttacatc
    20761 aacttctgca tctaaacttc ttgcaataac cctttgaaaa agagaaggag catttacaaa
    20821 tccaagtatt ctatttgcaa catttaatga agacataaca tcatcaaaat catccatttg
    20881 cccattagca acaatatatc ctatacgttc accaggtaga gataatgatt tactataaga
    20941 gtatcctata aatgaatttc tatagtattt tagtacacaa ggaactttgg caccatcata
    21001 aattatttct ctatatggct catcagaaat taggtatatg cttgtattaa attctttttg
    21061 ttttgaatcc aataattctc caagattttt taataattct tcagaatata tgacaccagt
    21121 aggattattt ggagtgttta ttattaaagc tcttgttttt ggagtaatag cattttttag
    21181 agcatctaaa tctggttcaa aagtctctat atttgtagga acttctatta attttccatc
    21241 atagttttct gtataatttt tatactcacc aaaatacggt gcaaaagcaa taacttcatc
    21301 accaggattt aaaagagttt ttaaaataat atttagacca ccagcagcac cacaagtcat
    21361 aattaaattt tctcttgtaa gatttaatcc atctttttta tttatgtgtt cagcaatagc
    21421 atctcttaca tcctcatatc ctgagttgtt catatatcca tgaactaaat ttggagattc
    21481 ttcatttaaa atatcattta taactgcttt aatagtctct ggaggttcaa cacttggatt
    21541 tcctatacta aaatcaaaaa cattttcctc accataaata tcagaaagtt tctttccttc
    21601 ttcaaacata gctcttataa cagaactatt tgcaactaaa gtttgcattt tattagatat
    21661 catatgtaac actccttttt atagatttat attgcttatg tatatattac catattttta
    21721 ctgaatttag acaattttcg aaaaattact tgtttgaaaa tata
//
LOCUS       DAYAOY010000071        21644 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYAOY010000071 DAYAOY010000000
VERSION     DAYAOY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.071"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(182..376)
                     /locus_tag="ACGI6B_002691"
     CDS             complement(182..376)
                     /locus_tag="ACGI6B_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9752126.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(750..2369)
                     /locus_tag="ACGI6B_002692"
     CDS             complement(750..2369)
                     /locus_tag="ACGI6B_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFG9752127.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(2678..2896)
                     /locus_tag="ACGI6B_002693"
     CDS             complement(2678..2896)
                     /locus_tag="ACGI6B_002693"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752128.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            3189..3380
                     /locus_tag="ACGI6B_002694"
     CDS             3189..3380
                     /locus_tag="ACGI6B_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752129.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(3469..4281)
                     /locus_tag="ACGI6B_002695"
     CDS             complement(3469..4281)
                     /locus_tag="ACGI6B_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HFG9752130.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            4703..5854
                     /locus_tag="ACGI6B_002696"
     CDS             4703..5854
                     /locus_tag="ACGI6B_002696"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9752131.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            5972..6610
                     /locus_tag="ACGI6B_002697"
     CDS             5972..6610
                     /locus_tag="ACGI6B_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HFG9752132.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            6611..7633
                     /locus_tag="ACGI6B_002698"
     CDS             6611..7633
                     /locus_tag="ACGI6B_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFG9752133.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            7623..8243
                     /locus_tag="ACGI6B_002699"
     CDS             7623..8243
                     /locus_tag="ACGI6B_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752134.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(8367..9053)
                     /locus_tag="ACGI6B_002700"
     CDS             complement(8367..9053)
                     /locus_tag="ACGI6B_002700"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFG9752135.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(9067..9612)
                     /locus_tag="ACGI6B_002701"
     CDS             complement(9067..9612)
                     /locus_tag="ACGI6B_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752136.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            9840..11087
                     /locus_tag="ACGI6B_002702"
     CDS             9840..11087
                     /locus_tag="ACGI6B_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9752137.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            11174..13168
                     /locus_tag="ACGI6B_002703"
     CDS             11174..13168
                     /locus_tag="ACGI6B_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9752138.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            13168..14490
                     /locus_tag="ACGI6B_002704"
     CDS             13168..14490
                     /locus_tag="ACGI6B_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFG9752139.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            14769..16055
                     /gene="grdG"
                     /locus_tag="ACGI6B_002705"
     CDS             14769..16055
                     /gene="grdG"
                     /locus_tag="ACGI6B_002705"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HFG9752140.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            16141..17448
                     /gene="grdF"
                     /locus_tag="ACGI6B_002706"
     CDS             16141..17448
                     /gene="grdF"
                     /locus_tag="ACGI6B_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:17188..17190,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HFG9752141.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            17725..18456
                     /locus_tag="ACGI6B_002707"
     CDS             17725..18456
                     /locus_tag="ACGI6B_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9752142.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            18458..19816
                     /locus_tag="ACGI6B_002708"
     CDS             18458..19816
                     /locus_tag="ACGI6B_002708"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9752143.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            19906..20340
                     /locus_tag="ACGI6B_002709"
     CDS             19906..20340
                     /locus_tag="ACGI6B_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752144.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKALQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(20603..20839)
                     /locus_tag="ACGI6B_002710"
     CDS             complement(20603..20839)
                     /locus_tag="ACGI6B_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752145.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
     gene            complement(20995..21219)
                     /locus_tag="ACGI6B_002711"
     CDS             complement(20995..21219)
                     /locus_tag="ACGI6B_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9752146.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(21316..>21644)
                     /locus_tag="ACGI6B_002712"
     CDS             complement(21316..>21644)
                     /locus_tag="ACGI6B_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFG9752147.1"
                     /translation="LMNIDLFELSTLLPPILLIVTFQVIFIILFSIFICFRVLGKDFD
                     SAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLVVPLVSGFLLDAISIPCILF
                     FINILT"
BASE COUNT         8530 a         2390 c         3364 g         7360 t
ORIGIN      
        1 attccagttt gtagggtaat tttgataaat aatattaata agtatgagta aaaagtagga
       61 gcatattaat ataatgtccc tactttttta ttattaaaat tgtttataat atcaacacta
      121 cattaaaaca caacctaaat ttgaaaaaca aatagtttag aaatatgtac ttttttatct
      181 attatattct tttaaatcga attttttcat ctgaacataa agtctcaaat ctatatgcgt
      241 ctgtcatatc ataaaaacca aaaaatataa agccatcacg agaagtaaat tcatattgta
      301 tattttcaat tttcaacatg ttttctactt cgcttatttt ttcgctatat ataatttcta
      361 ccatataatt tatcattgta gaatccctcc tattaatatg aaaatatcca aatatataat
      421 aacatattta gatgatataa tagtatccaa ataaaaaata atattattac attattgaat
      481 gaagaattaa aatgaagata aataaaacaa atattggtta taattgatta aaaaatagtt
      541 atgtatactt gtgactttat ttatgggcag ttcaagtttt atgaaagaag tcatatatat
      601 atggaatatt ctaggcagaa actgtttaat aataagagaa tactataaaa atttgagtat
      661 tacataaaaa ataagaagcc ataaatgata taagcattat ctatagcttc ttacttctca
      721 ttgattaaga ttatatataa aaataatcac tatatttttt gtttaagttt tagtacttgt
      781 tttactatat ttgttgagtg gtcagaaatt ctttctagat tagtaagtaa gtcaagataa
      841 agtacaccag catcaattgt acaacttcca ttatttagtc taatcatatg tgaatttcta
      901 aaagtttttt ctaaataatt tacttcattt tcaacttcta aaactttatt tgctaactca
      961 aaatctttaa catctaaaac tattaaggca tccttaaaat tagtaattgt cttattgtat
     1021 atttcgtata tttctcttat cgcactatca gaaaaactaa tgttcatttc cttaacactc
     1081 atggccaatt cagctatatt ttctgcatgg tcagagactc tttcaatgtc atttacagta
     1141 ttaaaaagca aatctgttct aaagcgttca tcatctctaa gaggttcttt agaaagtttt
     1201 aacaaatagt tcaatatatc tttctgtaac ttattgacaa ctttttctga ctcaaaagct
     1261 ttattaatat cttcattgct cttatttaaa aatcctgcaa ttgaatgttc caaagagttt
     1321 gtagctttat gacccattct tacaacttct tgtacagtat taccaagtgc tatagatgga
     1381 gtgctaaaca tcctttcatc taagtactta gtattattta ctatttctga ttcagtttct
     1441 ttaataggta taagtctatt tgctaattta actatgtatt tagaaaatgg taataatatt
     1501 ataacatttg taagattaaa taaagtatgt gcatttgcta tttgtctagc tgtatcagtt
     1561 gggtcaaaat gagtaactat ggctgatata ggtttattta aaacaagcat aaatattatt
     1621 gtaccaatta cattaaaact taaatgcatt acagctgcac gtcgagcatt tctacttgca
     1681 ccaacacttg ataaaagtga agtaacacaa gtacctatat tatctccata aagaataggt
     1741 aaagcagatg atagaggaag tattccttga gaagctagtg ctattaacat acccatagat
     1801 gcacttgaac tttgaacaat accagttatg gcaaatcctg ccaatatacc taatacagga
     1861 tttttactaa aatataaaag tgcatcagtg aatgctttat attcagcaag tggagctact
     1921 gcatctttca taaactccat tcctgtaaat aatataccga aacctataag tatctcagat
     1981 aaatgctttg ttttttgatt tgatgtaaat aaatagaata aaatacctat acctaaagca
     2041 attggtgcaa ttccttcaag attaaatgaa actaattgag cagtaactgt agtacctata
     2101 tttgccccca ttattacacc aatagcttga cttagattca ttatacctgc atttacaaat
     2161 cctacaacca taacagtagt agcacttgaa ctttgtataa tacctgtaac gactgtacct
     2221 accagtacac ccataacaac attacttgtt aagagttcaa taattttttt taatttgtct
     2281 cctgctgatt tttgtaaacc ttctcccatt aaactcattc cgtaaagaaa aagtccaagt
     2341 ccaccgataa ggctaattac tatattcacg atatttcctc ccttaagaaa ttaataattt
     2401 ttttatctga attataaact ttattaacta aaatttattg tttccataat atagaataac
     2461 aaattttgtc aataaaaata ttaatgtttt gttaagtata cgttaagaga atgttaaatt
     2521 tttaagttgt tgttaatttt agaaaattta tgatatatag caatttaaat acttgaactt
     2581 agatttttag tattttatgt acaatcaatt taacaaagaa ttaactataa ttgtaaaaaa
     2641 tatgtaaaaa aataacatcc ttttagaatg atatttttta gttataaata tttttatatt
     2701 caattggtgt aaagttatta aactttttaa aaattatact atagtaattt tgactattga
     2761 atccaattag tacagcaacg tctaaaatag acaagtcttt ttttaatata tactttttgc
     2821 ttttttcaac tctaacttta cttaaaaaat tagtaaaagt atatccactt tttttcttaa
     2881 ataaagaaaa aaacaacttt tattaagttg taattcatta catatattac ttactgttat
     2941 atcttctgag taatttttgt gaatataatc tatagttttc tttaagtttt ctttacataa
     3001 aactaagagt tggtctatgt ttcattttat caaggcatat ttattaaaaa atcaatctct
     3061 atttgttaaa ataatttatg aatgatgtta gaaactgtaa tatattggat ataatataat
     3121 aaacagataa actctgtatt aagaggatat atagtttatc ttaattatag gggaagtgag
     3181 gatatattat ggaaagatta atggaactca tagaaagaaa atttttaaag gatgtagaac
     3241 ttttagaatt agaagacata gaaaatgtaa tagaatgcaa aatgacacag ccatataatg
     3301 ctcattataa tgaatattta gtaaaagtaa aaaatggaga tgaagtagaa gaatattttg
     3361 tgtatttaaa acatagataa acataaaaga ggtttatatt tatttgtatg attataaaat
     3421 aaatacaagc ctcttttttt gataaatata aaattataaa atttttattt atttaatttt
     3481 ttcaacaaca ttagtcaata ttgaatctaa atcatcacta atttctgtaa ctccattatg
     3541 agtaatatct ataatttctt tagtcatagg aagttctcct aaaagctcta agtctaaatc
     3601 atctaaaaac ttttcagtag attctccttc aaataacttg atttttttag aacaatcagg
     3661 acattggata taactcatat tttcaataac tccaaggaca tttatgttca tttttttagc
     3721 catattaaca gcttttgata caatcataga taccaaatct tgaggtactg aaaccataac
     3781 tattccactt ataggaatag attgcataac tgttaaggca acatctccag taccaggagg
     3841 catatctatt aataaatagt ctaattcttc ccaaagaaca tcagtataaa attgctttac
     3901 aacccctcca agtaaaggtc ctctccaaac aacaggttca ttttcatcat ctatcataag
     3961 gtttatagac attactttta tgttatttga gttttctaca gggtaaatat aagagccatc
     4021 agaataagct tttacatttt ttacacccat aagtctagga atacttggtc cagttatatc
     4081 tgagtctaat ataccaacct tatatcccat tttgtttagc ttattagcaa gtaaagcagt
     4141 tacagttgat tttccaactc cacccttccc actcataaca ccaattattt ttccaaattt
     4201 attgttaggg ttattttcaa tagaacaatt tgattgacta ttacaatttc cctttgatgg
     4261 acatgaatta caatctgcca tatctattac ctccaaactt taatatttat tagattattt
     4321 gtaacctaca tatactattt tagtacaatt gaaccataaa gtcattattt aaaagcaact
     4381 tagaagattt tctattgatt tattcataga ctatagcaaa aagaatacaa taaaaaaatt
     4441 ataacattct tgaaaaagtg attatttcaa tttagttata gaacttagtc atataaatat
     4501 atagaaaata ttttaatatg ttatacataa cgggttattt tataagttta actataaatt
     4561 aggtttatat tttataaaaa tttgctaaaa aaaacaatta aaaattgtaa agaaaaaaga
     4621 tataattaga actgtagtaa taaatgtttt attatagata atagagtttt aagtatctta
     4681 aataatattt ggaggaatat gaatggaatt aagaccaaga agtgcaaaat tagaagaatt
     4741 tgataaagta atagaattaa taaactatgt atttagaata tcaagaaatc ataaaccaac
     4801 tatgatggaa gaatttcctc ttttactaag taaaaataat atagaaaata tgattataat
     4861 atcagaagat gataaagtga tttctgatgt aaactatcta attcaggatg tatctattca
     4921 aggaaacaga ttgaaggttg cagctatagg tggagtatgt actcatccag attatgaaaa
     4981 gagaggctat tcatctaaga tattggataa agtagaagaa aaaatgtttt atgatggtgt
     5041 agatattgta attatatcag gaacaagaag tctatacagt agaagaaatt gtagtttggt
     5101 aaagagtttt tataaatata caataaaacc tgaagatgta aaaatagcat atgaaatagt
     5161 tgaatttgat gaaactaatt ttgaaaaaga taatgattta gataaaatga ttgaactata
     5221 taatcaaaat agtactagat ttataagaac gagagatgag tttcaaaaac tattgcatgc
     5281 agcaactatt gcctggggac caattggtta caagaaggta tttataaagg aaaataataa
     5341 tataatagga tatttgataa taagaacaat aaaaaaagaa gattctactg taggagaagt
     5401 tgctgaaata gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg ttgcaaataa
     5461 atttggatta gaatatttaa attataaagt acatgtgaaa aatcttaaag accagttgaa
     5521 atgtaatggg acaaaatctt tggattatca acaaggaact atgaaaataa taaattttac
     5581 taagttgtgt gatagtttaa gaagttattt tagtcaatat gtagattttg aattactaaa
     5641 atacatggaa tttaaacaag ttgaaaataa atatattatt aaatacaaag aagaagaact
     5701 tgtaatagaa aatttggata agcttaataa actgtttttt gaaaaaaatg aggaacagta
     5761 caatgaattt aaacatctaa aaaatatata tgaatttgct acaaaggcat ttcctgtaga
     5821 ttttccatgg actgcaaatt taaattatca atagttttag atgatattaa ataaaattga
     5881 ttgataccta gagttagtcc ttgttaaata agagaaatta ttggatattg aaaaagcaat
     5941 tttgtaagta attagaaagg atgagtatta tatggacata cttgatgata tagcagaatg
     6001 tatattaaat atgggtgtag ataatataga gaatttagta aaaattgcta tagataaaaa
     6061 tattgatgtg gaagatattt atgaatatgg tttaaacaaa ggtatgatag gagctttaga
     6121 taagtttgag aataaggaat actatctttc agaagttata gtatgcacag atgcattaaa
     6181 taaaggtatt aatcttttga agggaactgg aaaagtaaag aaaaaatcaa agggagtaat
     6241 tctgatgtct gtagtagaag gagatactca tgagatagga aaaaatattg taaaagtaat
     6301 ggtagaagcc actggatata aggttattga cttaggtgta aatagaaaaa gtgaagatat
     6361 aatagaagaa gctataaaaa acaatgtaga tataataggc ttatcttcaa tgatgacaac
     6421 tacaatggaa aatatgaaat cggttataga cgaacttaat atgattgaga tagataaaag
     6481 accaaaagta ataattggtg gaggacctgt aagtatggag tttgcagaag aaataggagc
     6541 agatggatac agttcaaatg ctccaaaagc agttaaatta ataaataaac ttataggagg
     6601 tgaagtttaa atgttttcag atatgacttt acttcagtat ataaatagtg aagatagagg
     6661 tttttttctg cctgatatgg gtacaaatgg attgttctta ataaataaaa aggcatatga
     6721 agtatatgat agtgtagatt tgcaattaga attagcaaag actatggaca agtattttga
     6781 atcagatttt atatactcat tctgtgatgg gattacattt tgtgaaactc tgggtttaga
     6841 gacattaaga ccagattatg attttccaag tgtattgaca catactataa ctgacagaga
     6901 taaattaaga aaattagatg ttccagaccc ttatacgagt ggacgtatgc cactcaatat
     6961 agagagctta tcagttatat caaaaagtat taaaaaacca ctatatgaat cgatacaagg
     7021 tccatttaca cttgcaggtc aattagctgg agctactcaa cttttaagat gtataataac
     7081 tgataaaaaa tttgtagaag aattattgga atttacaact gaattggtaa gaagatatgc
     7141 tgtagctgca aataaagcag gagcaaaata tatttcaata gcagagccaa catcagtaac
     7201 cctaagtaaa gatagatttg atgaatttat agtaaaaaat ttaaataaaa tctacaatga
     7261 acttgattgt tggaaaggta tgcatatatg tggagacacc agagaattat tagataatat
     7321 gctaagttgt aatatagatg cagtaagttt agaccaaatt ttagactatg aagaaattgc
     7381 tccaatgatt cctagagata tagttttgat tggtaattta gacccaataa aacttcttgg
     7441 acgttctaag ccagataaga ttagaagaga gacgctaaaa ttgttaaaga agatgagaag
     7501 atatgataac tttttatgtg attttggctg taactgctta aataccactc cagtagaaaa
     7561 tttacaagca gctataaagg caggaagaat tagttataaa gaattagata ggattgatat
     7621 tgatgaatta taatagtaat aaaaataaat atcttttaaa ggatataaat ttagatgata
     7681 tttttataga taagaatatt gtgctaaagt ttttggggta tggaaataga aaagctccag
     7741 atacaattag taaaatcgtt gaaagagaaa taagaaatat aaatgatatt ttagatatca
     7801 agatatatat aagtgaagtt gatattaata aaacacctaa agaatgtaaa aaagcatttg
     7861 ctgtactata tacaataggg gataaaattg atattaagat gaatgactat atggaaaatt
     7921 gcaatatgat ggcagggcta gcattggata aaataggtat agtatgttta gattatatta
     7981 atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag tctcaaaata tcgcatgaaa
     8041 tatatccagg agataaagat tttgaagtag agagacaaaa ggatatttat aactatgtaa
     8101 aaaataaata taatagtata gaaatagaaa ttaatgatta tcatcaacta tcccccataa
     8161 aaagtgttgc aatgcttatt ttaatggggg atgaagaaaa tttagaatca agatgtagta
     8221 agtgtcctag aaaatgcttt tagttaattt tcaagactaa gatttgtttt gtgtataaat
     8281 tgcaataatt tttttggtca ttttatgtat aaactaaaaa aattattgca aagtaatatt
     8341 ctatttaatt tgaacagact tacaaactat agtagtttct ttaattcaat aagtgtattt
     8401 atttcataag ttggtatatg attagtataa tttatactgt tattggaatt taaccaacaa
     8461 gtatctattc ctgaatttat tccacctaag acatcagagg ttaagctatc cccaattatt
     8521 aaggcagaag atttatcatg acaattaaac tttttgagag cgtatttaaa tatttcagga
     8581 tttggttttg atattttcaa ctcttcagat acaaccattc catcaatata tccttttatt
     8641 tttgaatttt caagtctttt attttgaact gcagaaattc catttgttat tataacaata
     8701 gtatactttt ttttaagata ttgtaatata tcgattgcac ctggaattaa aaatgtacat
     8761 tcacctaaaa aatttaagta cattttgcta agagtttcag aatcaacatc caaagatatt
     8821 ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat tcaaagttat aatatttttt
     8881 tcaaggtcga gccaaagctt atcacttata tttctatatg actctatata attctcaaaa
     8941 ttaaattcta aatcaaactc agatagaagt tttttaaaag catgtgattc acttttctta
     9001 aaatcaaata aagtatcatc tgcatcaaaa aatataaatt tatatgtctt catggttttt
     9061 actcctttac aagatgactg caaaaattac tttactttca ctatttgagt cattttgcca
     9121 tttatgtttt gttcctaaag gaacttttac actatctcct ttggacaaag taaattcatc
     9181 atcatccata aaaagtttaa cttctccctc caaaacatat gcaatttcat ctccattatg
     9241 agtaatccta tcaacacaag aagaagtgtt tggagctaaa tccataattg caaattctaa
     9301 attaccacta gagtctggaa ttaaaatctc atatataact tctttgcttc caggtaaaat
     9361 aacttttttt ctattatcat gtcttactat caagtttttt ttatctactt cagatacaaa
     9421 aaatgtaaat aaaggaacat ttagagaaga ggctatagat tttaatgaat ttacagatgg
     9481 attagcaaga cctcgttcta tttggctcaa taatgatggt gtaacatcag ctagttttgc
     9541 taaatctcga atacttaagt tttgtttttt tcgaacttca gctatttttt cccctaaatt
     9601 taaatcattc atataaatat cctctctttt taatttaaaa aatattaact taaaataatc
     9661 aagtaaaatg attatgtagt atgaaattat tataacatat agaattaggt tttgaaaaaa
     9721 ctatgttcaa tataattaaa taaattaaaa tataattgac aaagtggata cagaatactt
     9781 aaaatatatt taaataaaat taagcaaaag atagttaatt tatgatagga ggggatatta
     9841 tgcaaataac gacttttgga gctatatttg gtttattaat agcaattatt ttaattataa
     9901 aaaaatttca agctgtatat agtttaatgt taggagcatt tattggagga ttagttggag
     9961 gagcaaatat aacacaaact gtagatttta tggcaaatgg agctatgaat atatcaccat
    10021 caattcttag agctttggca tcaggtgtat tagctggaag tttaataaaa acaggggcag
    10081 tagataaaat atcagaacag attgtaaaaa tatttggtga aaagagggct ttattttcga
    10141 ttgccatttc aactatggta cttgctggag taggtgtaaa tttggatgta tctataataa
    10201 ctgtagcacc tataggatta tatataggta gaaaattaaa ttattctaaa ttgtcaatac
    10261 tattagctat gttgggaggg ggtaaagcag gtaatataat atcaccaaat ccaaatacta
    10321 tagcagtagc agataatttt tctgtcaatc tctctagtgt tatgatggca aatataattc
    10381 cagcaattat tggagttgtt attacagtta ttttagcaag tatattaata aataaaggaa
    10441 ataaagtaca atcatatgaa attttagagc aaagagaaga tttaccaagt ttatttaaat
    10501 cattatgtgg tccaattata gctatatttc tattgttttt aggaaatgta tctcctatag
    10561 ttatagaccc aatgatagcc cttccaatag gaggaatcgt aactctaatt gtcacaggaa
    10621 acttgaacaa ttcaagagaa tatttaactt ttggattatc taagatgcaa ggggtatgca
    10681 tattattact tggaacaggg actatcgcag gaattataca gatgtctgaa ttacaacaga
    10741 gtacaatagg tgctttacaa tttttaaata tgccacaatt tttattggca ccagtttcgg
    10801 gtatacttat gtcattagca acagcatcat caacagctgg tgctactata gcatcatcta
    10861 cttttcatga tgctataata aatggtgggc tatcaccgat ttcaggtgca tcaatagtaa
    10921 atgctgggtc tagtgtattc gagcaattac cacatggctc actatttcat actagtgcag
    10981 gtagtataaa catggatata ggtgaaagat ttaagttaat tccatatgaa gcattgatag
    11041 gaatagtaat gactataatt tcaacatcta ttcaattagt tttataataa atataaatat
    11101 ttaatgaaaa aagtaggatt ttgttattaa atattgaact tattatagta aaaaagaaga
    11161 ttggagtttt aaagtgaata aaaaaaagat tgttattata gggattattt attcattttt
    11221 agtagtattt tcacttacaa atatgtatgt aaatatggag tataatctaa atgtatttga
    11281 atatattaag aagtcacttc catttacaga agaagaaaaa aagtggttag aaaaacacaa
    11341 gaatttgatt tacagctctg accaaagttc acctcctttg agatataaag gtaaggaaga
    11401 tggacagtat aagggaatta ttgttgattt aataaattct ctgtcaattc aaattggtag
    11461 agatttttat tttaaaccaa ataattggtg gaaagaatct ttcgtaaatt caatagatga
    11521 tagtataaag ttttttgact tgattccatc aaaagaaagg gcaaataaat ttatatttac
    11581 tgacccaata tacactttga gtgcaaatat attgaaagat aaaaagtccc aagatataaa
    11641 tagttacatg gatttaaagg ggaaaacagt agcaattcca gaaggtgatt attctataaa
    11701 ttttttaaag caaaaaattc aagatataaa cattttattg actccagata taaagactgg
    11761 agtcaaccat ttaatgtctg gaaaagtaga cgcagttgtt ggtgatgaac cagtattgcg
    11821 atactatata aataattatg gactttcaaa taaatacagt gttttaagta atcctatata
    11881 tactaaaaaa gcagttcttg ctgtacctaa acaatatgaa gagctggtta gtattttaaa
    11941 taaaggcatt tttaaacttc agaaaaatgg agtgtataaa gatttaaaga aaaaatggta
    12001 ttctacgtat aatgaagttg atgatatatt gtatgagcga ggaattgtgc ctagtattta
    12061 tctttttata ggtataattt taatatcaat ttatgttttt tatagctata catatttgtt
    12121 aaagatagaa atcaaaagga aaacagaaca ggtaatagaa aataaaaaaa ctttagaggc
    12181 aacttttaat agtattacag atataataat gcttgtagat gaaaataaca atatagtaga
    12241 atcgaacaaa gtactatatg actttatggg tgaaatgagt tataagatag cagatttgat
    12301 tagcatgata aaaggtgtta ttgaaaatac cttctctgaa aatactaata aaactagtga
    12361 aatagaaata cacaataaaa ttttaaagat aaacactttt cctgtagagt ataaaaaaaa
    12421 taatactgag tatatagtag ttcttattaa agatataact aatgataaaa tagtagaggc
    12481 gaaattactt agagaaaaca aaatgatatc tatagggcaa ttagcttctg gggtagcaca
    12541 tgagataaga aatcctttgg gtataataag aaataattgc tatctcttaa aagataacgt
    12601 tacaatggaa gaagtaaatg attgtgttaa atcaatagaa agtaatgtag atagggcaag
    12661 taatataata accaacttgt taaatttcgc aagaatatca gatgataatt tagaacatat
    12721 aaatataaaa aattttattg aaaatatagt gaaacttcaa tataaaatgt tacagttaaa
    12781 gaatgttgaa ataaaaattg actgtgaaca taatttaata tgctatataa atggagaatc
    12841 gttaaaacat gtatttataa atttaatttc taattcaata gatgcaatac atcaagatgg
    12901 aaaaattatt atatactgtt atgaaaaaaa tcattgttta tttatagatt ttaaagataa
    12961 tggtgaagga ataaaagaag atgtcctaaa ggacatattt aatcccttct atacaacaaa
    13021 accaattggt gagggaactg gtttaggttt atatattact tataatgaga ttaaaaagaa
    13081 taatggagat ataagtgttg aaagtaaatt gggggttgga acttgttttc atataaaaat
    13141 tccattaaat aaagaggtga ctatataatg aaaatactat tagttgatga tgagctagaa
    13201 tatggagtgg taatgaaaaa aattctacaa aagaagggct atcttgtaga tatagcacta
    13261 agtggtgaag aagctataaa tataattaaa aaagataaaa attatgattt ggttttatct
    13321 gatgttatga tgaaaaatat ggatggagta cagctattag atagaattaa atctattaat
    13381 agagatatag aggttatatt agttacagga tatggaagta ttgaaaatgc agttgatgct
    13441 atgaaaagag gagctttatc ttattttata aaaagtaatc ccattgaaaa tttattagaa
    13501 gaagttgaaa aggtaaaggc aagtaaaata tctgtttctg tacaaaaaaa taattttgaa
    13561 tttactttgg aatctaaaaa tagagatttt aataatatca taaaaatagc aaaaaaagct
    13621 gcttgtaaag atgttaatat attaatcttg ggggaatctg gtgtaggaaa agatatatta
    13681 gctagatata tacatagcat aagtccaaga aagaatgaga tatttgtacc tgtaaattgt
    13741 tgttcttttt cagaaaatct attggagtct gagctgtttg gtcatgaaaa aggttctttt
    13801 acaggagcag tagatagtag aaaaggtaga tttgaacttt cgaataaagg aactctattt
    13861 ttggatgaaa taggagatat acctctaaat gttcaggtaa aacttttgag gacacttgag
    13921 gacaaatcta tagagaggat aggaagtaat aaatctatta aagttgactt taggttaatt
    13981 tgtgctatga ataaagaacc aaaagtggaa atatcaaatg gaaatataag agaagatttt
    14041 ttttatagaa taagtacaat aacaataact ataccaccac taagaaagag aagagaagac
    14101 ttaactactc ttatagaatt tttcttaaat aagtaccaaa ttgaacatga taaaaagata
    14161 cactcgattg ataaggaagt aaaggatttt ttattaaatt acaattatcc aggaaatata
    14221 agagaattaa aaaatataat aaatagactt gtagttttaa gtgaagaagg taatttatca
    14281 aaagacaatt taaatttaat atcaaataat gtatacatag atgataaaat aagtattaga
    14341 cctttaagag aaataagaaa agagtttgaa tgtgagtata ttgaaaaagt tttatctttg
    14401 tgtggaaata atatttcaaa tacagctaaa aaattggaga ttagtagaag acagttaacc
    14461 aataaaatat cagagtataa tattaagtga gaaaaaaact tctcattaag tgagaaaaaa
    14521 atttctcaaa ctgtattaaa agataaacag aaaaaatctg tttatctttt tttattataa
    14581 ttttaaaaat taatatttac aataattatt gctaaattca atattggtgt aaattaaaaa
    14641 tgaaaatttc tatgacatta atacatagaa tatatatttt aaataattgg catagaaaat
    14701 gctttatata ttaataaacg taataaatac ttttaattgc gattaaatat attaaggggg
    14761 attttcatat gaagctagag ctagggaata tatttataaa aaatgttgaa tttggagaaa
    14821 agacagaagt aaaagatgga gtactatata ttagtagcga agaaatagaa aagatagtat
    14881 tagaagatga aaggattgta agtgtaaatg ttgaacttgc aagacctggc gaatcaataa
    14941 gaatagctcc agttaaagat gtaatagagc ctagagttaa aattggagat gaaagcaaaa
    15001 tatttcctgg aattataaat aaagtgaaga ctgtaggaag tggaagaacc catgtacttt
    15061 taggagcttg tgttgtaact tgtggaaata tagttggatt tcaagaaggc gtaattgata
    15121 tgagtggtcc tactgctaaa tatacacctt tttcaaaaac aaataatata tgtattgtta
    15181 taaaagcaaa ggatggtata gatactcatg attatgaaga agcagctaga attgcaggat
    15241 taaaaatagg tgcatatgta ggtgaggcag gaagagaagt tgagccagat gaagtcattg
    15301 tatatgagac aaagccatta ttaaaacaag taaaagaata tccagacctg ccaacagtag
    15361 catatgttca catgttacaa tcacaaggac ttttacatga cacttattac tatggggtgg
    15421 atgctaaaca gatagtgcct acatttatgt atccaactga aataatggat ggagctataa
    15481 tatctggaaa ctgtgtagca ccatgtgata aggttacaac attccatcat ttaaataatc
    15541 cagttataca tgacttatat aaaagacatg gaaaagattt aaactttata ggagttattt
    15601 tgactaatga gaacgtattc ttagtcgata aagagagaag ctctgatatg gtagctaaat
    15661 taatagaatt tttaggagtt gatggagttc tagtgacaga agaaggttat gggaatccag
    15721 atactgacct aatgatgaat tgtagaaaat gtagtgaagt tggagcaaat gtagttttga
    15781 taacggatga atttcctgga aaagatggca agagtcaatc tatagcagac gctactaagg
    15841 aagcagatgc agttgtttct tgtggtcaag gaaatctagt ggttcatttc ccagcaatgg
    15901 aaaaaattat aggaacttta gattatgttg aaatgatgat aggtgggtat aaaggatgct
    15961 taaatgaaga cggaagcatg gatgcagagc tacaaattat aatagcatca actatagcaa
    16021 atggctataa tcatttgaca gcaagatatt attaaataag tatttgaata taaagttctg
    16081 ttgatgtaat cattggtcta actgatgtaa taactataaa gtgatttgga ggattaagag
    16141 atgagtaaaa tcagagtggt tcattatata aacaatttct ttgctggtat aggtggagag
    16201 gaaaaagcag atattccgcc agaaaaaagg gctggtgtag taggacctgg aatagcattt
    16261 cagagtcaat ttaatgatga tgcagagata atctcaactg taatttgcgg agatacgtat
    16321 tttggtgaaa atattgagag tgcaactcaa gaacttttaa aaatgataaa agaagaaagc
    16381 cctgatttat ttattgcagg cccagcattt aatgcaggta gatatggagt agcttgtggg
    16441 actatttgta aggcagttga agaagagtta aatattcctg taattacagg catgtataaa
    16501 gaaaatccag gtgtagatat gtttaaatta gatttgcata tagtgtctac aggaaattca
    16561 gcagcaagtc ttagaaaggt agttccaata atgacgaaac taggtcttaa attggttaga
    16621 ggtgaggaaa taggacctcc tgaagaggaa ggatatataa tgagaggaat aagaaaaaat
    16681 ttcttccatg aacttagagg gtctgaaaga gcaattgata tgttggttaa aaagatgaaa
    16741 ggggaaagct ttgaaactga gtatccaatg cctgaatttg atagagttac acctatgaaa
    16801 cctgttaaag atttatctaa gataaaactt gccctagtaa cttctggagg gatagttcca
    16861 attgataatc cagacaaaat tgaatcttca agtgctacta aatatggaat ttacgattta
    16921 acagatatgg attctatgtc aaataaagaa tttacaacta tacatggggg gtatgacaga
    16981 gcatatgtcc ttgaaaatcc aaatttagtt gtaccactag atgtagttag agagcttgaa
    17041 aaagaaggtg taattggaga attagtagat tattttataa ctactacagg aacaggaacg
    17101 agtgttggca attctaagag atttggagaa gagttttcta agaaattgtt agaagatgat
    17161 gtagatgctg taattttaac ttctacatga ggtacttgta ctcgttgtgg agcaacgatg
    17221 gtaaaagaga ttgagagagc tggtatagca gttgttcata tatgtacagt agtaccaata
    17281 tcacttacaa taggagctaa tagaattgtt ccagcagtag ggataccata tccacttgga
    17341 aatcctatgt taggagaaga ggaaagtaaa aaaattagaa agaatatagt tttaaaagct
    17401 ttaaatgcac taacagaaga tatagaagaa caaacagtat ttgaataata aaaatacaac
    17461 acaacactga aataaaaaaa gatatcaaaa aataattttt taaaattatt ttacgatatc
    17521 tttttttatt aggtaaattc gaaatttgca tttttttaaa ttaaatttat aaaagtgata
    17581 aattagttta ttataatatt gatacacaaa ttcaaattag atatactatg aaaagcgaga
    17641 ttattacatt ttggaacaaa tattcaacat attagatcat attaattgaa cagaaaaaat
    17701 taactttata taataattag aataatgata agaaagaagg tgtatatatt gatttcaatt
    17761 gccatttgtg atgatgaatc ctatatgcta aatcatttaa aggaaaatat agaattatac
    17821 atgaagcaga agaatttatt atataatata gagcttttta ataaagtaga agatttacta
    17881 tgcaatcatg aaaagtttaa tattgtattt ttagatatac agatggaaga gattaacgga
    17941 atgcaggcag ctagaaaatt aaggtcatat ggtaatgagt gctttataat tttcgttaca
    18001 gttttaaatg aatttgtatt tgatgctttt gaagtagatg ctataaatta tctgataaaa
    18061 cctctatcta atgagaaact atataaaact ttggataaaa tagttacaaa acttgatgat
    18121 aatgaaagta attatatcac aatacaaaaa ggttattcat attggaaatt aaaacttgct
    18181 gatattttat attgtgaagt tataaaaaga aagatttatg ttcatttaaa gaaagagact
    18241 gtagattatt atgagactat tgaaaattta gagagaagtt taccaacaaa tttttttagg
    18301 tgtcatcgta gttatataat caatttacaa tatgtatatg gatatgaaaa tggtaatgca
    18361 gttatggaaa gtggagatag aatacctgtt tctagactac gtcaacaaat attttcaaaa
    18421 gttatgcttg agttcatgaa aaataggagg atataagatg attaatttat tttcttccga
    18481 tttattttta aagtacagtt ttttcttctc ttttattgta tatagaattt taatggtaat
    18541 agtgcaatac tattttctaa tcaattttac aaattttaat tcaaaatttt ggtgttttat
    18601 tttatattcg ataatttcaa tttttttaat cattataaat acatatttta atttatataa
    18661 tttattttct tcacagattc tattttttct tatgactttt ttatttgtaa agtacataat
    18721 aaaacaatct actgttttat ctatagtatc aactttgtta gtatatttaa ttgaacaatt
    18781 agtatatgga tttacagctc cattaaatta cttgatattt actagtgata taaatacaga
    18841 tatttataaa tttgatataa atatgggact atcttcttta attactatta taatagctgg
    18901 atttatatat tggtatagtt ctaaaaagtt taatattaag gtcatgaact ttgataaata
    18961 tatagcaatt ttgatgattc cattgttgct tataatatta tttatgcaat catttgaata
    19021 tagttcaaat ataaatatag atacaagtct tgggattgta aagctgctat taaatacttc
    19081 tataactgaa gaaattcaag cttttctttt ttctataatt ggaactatat gtgtattttt
    19141 ttctctattt acctttaaga aactaattca agctctcgaa gatgacaagg agagggcaat
    19201 tatgaatcaa cagattcatg ctcaaaaaaa ctatattgaa gaagcgaaat cacgattatc
    19261 acaaactata tcctttagac atgattttaa taatcattta gctattgtca atggattgtt
    19321 gaaaaaagac caaattttaa aagcacaaga ttatttaaat aagcttgaaa aagtaacaag
    19381 tgacctatca ttttcttgta atacacagaa tattgttatt gatgctcttt tcaacaataa
    19441 attaagtata gcaaaacaat taggcataga ggtagattgt gaagtaatta taccccaaag
    19501 cactaacatg aacgattttg acctatgtat agtgtttgct aatgctattg acaatgcaat
    19561 taaagcttgt agagttgtag ataaacaaaa taggtattta aagctttcca ctaagttaga
    19621 gggtggattt tttatgatag aaataaaaaa tagctataat ccaataaata attatacaag
    19681 tggctcaggt ataggtcttt taaatataaa agaaattgct aaaaaatatc aaggggctat
    19741 aagtatagat aagacatcag actattttca aataaatgta ttgctagtta tttcattaca
    19801 ttgcgctagt atttgagaac aatttcattg aaaatagtat ttgtaagcta tattatgtat
    19861 ttgaaaagag aaagtttgat atatagaaat gaggaggtta ctaaaatgaa agttggaaat
    19921 ataaatggtc aagcaccaat tacaaatggt ttagatagtt gtcaaagtga ggtacaaggc
    19981 ttagaacaaa agaaaaaagc attacaggat gaaatagaga aagtaagaag tgataaaagt
    20041 attgatggtg aaacggccaa attaaaaata gaagctttaa aaaaagaaat ggaagagatt
    20101 gaaaataaaa ttcaagagtt aaaaggaaat aaaggaaaaa aaacaaataa agaagaaaat
    20161 aacgtttcag aagtaaataa taatctatta gagagaaaaa aagaaccttc taataatgaa
    20221 aatgtaaaac aattatatga tgtttttgaa agaagcaatg tagacgaaca atgggataat
    20281 acttatcgtg ttctccaaga tgataaaaac ttaaaagtaa aatttaatag acctatatag
    20341 tttatttata aaaatagtta atattttaat tatcaccact ttttagaaga atacacactt
    20401 tattttaaag acccacatat ttatttacag ttagcacatt tatatgtggt tttataacta
    20461 ttatttacaa gtctatattg ataactttta catagtatat gatgtttaaa tacttattca
    20521 aatttatcta tacataaaca aaaaaagaga ttttaaaatc tctttttaga ttgttgactt
    20581 atggcttttt gatttaataa aatcaagaag ccgtttgaat tttatatgtt ggctgatacc
    20641 cttctgatat tgaaacacca tttttgtttc caaaatcgtt taaaattaag aacttattat
    20701 gaatgactat ttcattatga acaataagca aataacatga atcttcaatc aatgttttaa
    20761 cagtagcatt aaatgcttgc ttatatagtt ctatcgtttg tttgaactgc ccattaaagt
    20821 aaatatgagt caataacata actatatcta cacaaattaa tataaatttt ttatactgta
    20881 cattatctaa ctgttgatta tctgtattac aacatgattg aatttaaaaa gccatcaaca
    20941 catagaatgt tgatggcctg aaaatagatt ttaaaatctc ttttttatta tatattaatt
    21001 attttttaaa tttatatttt ttgcatcaga ttttctaata gctaagtcaa accctctaaa
    21061 tctaatcact atagggtcat taagaggagc tatctttttc acttcaactt cagtgccatc
    21121 tattagtcct aaggctagaa gtctcttcat aagtttttca tttccagcta tattgtctat
    21181 aattcccttt tgtccaagtt ttaagttgta aacagtcatt attaacacct cctaatgaaa
    21241 atcattatca aactttatat ttataatata taacaaaaag agcaaattgt caatatataa
    21301 caaaaaataa acatactaag ttagtatgtt tataaaaaat agtatacatg ggatactaat
    21361 agcatctagc aaaaaaccac ttactaatgg tacaactaaa aaggcttttg gagaatcacc
    21421 atatttttgt gtcaaagaac tcatatttgc cagagcgttc ggagtagctc ctattccatg
    21481 tccaatcaaa ccacttatta tgatggcaga atcaaaatct tttccaagta ctctaaagca
    21541 aataaatatt gaaaatagga ttataaagat tacttgaaaa gttactatta agagaatagg
    21601 aggtaataat gtagataatt caaataaatc tatattcata agtg
//
LOCUS       DAYAOY010000072        21438 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYAOY010000072 DAYAOY010000000
VERSION     DAYAOY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.072"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            263..2362
                     /gene="nrdE"
                     /locus_tag="ACGI6B_002713"
     CDS             263..2362
                     /gene="nrdE"
                     /locus_tag="ACGI6B_002713"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFG9752148.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2375..3346
                     /gene="nrdF"
                     /locus_tag="ACGI6B_002714"
     CDS             2375..3346
                     /gene="nrdF"
                     /locus_tag="ACGI6B_002714"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFG9752149.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3422..4048
                     /locus_tag="ACGI6B_002715"
     CDS             3422..4048
                     /locus_tag="ACGI6B_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9752150.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4443..5072
                     /locus_tag="ACGI6B_002716"
     CDS             4443..5072
                     /locus_tag="ACGI6B_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752151.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5073..5855
                     /locus_tag="ACGI6B_002717"
     CDS             5073..5855
                     /locus_tag="ACGI6B_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752152.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5848..6726
                     /locus_tag="ACGI6B_002718"
     CDS             5848..6726
                     /locus_tag="ACGI6B_002718"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752153.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6759..7718
                     /locus_tag="ACGI6B_002719"
     CDS             6759..7718
                     /locus_tag="ACGI6B_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9752154.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            8033..8725
                     /locus_tag="ACGI6B_002720"
     CDS             8033..8725
                     /locus_tag="ACGI6B_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9752155.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8966..9739
                     /locus_tag="ACGI6B_002721"
     CDS             8966..9739
                     /locus_tag="ACGI6B_002721"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9752156.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV"
     gene            10007..10858
                     /locus_tag="ACGI6B_002722"
     CDS             10007..10858
                     /locus_tag="ACGI6B_002722"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFG9752157.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            10962..11708
                     /locus_tag="ACGI6B_002723"
     CDS             10962..11708
                     /locus_tag="ACGI6B_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752158.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11708..13624
                     /locus_tag="ACGI6B_002724"
     CDS             11708..13624
                     /locus_tag="ACGI6B_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9752159.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            13904..14137
                     /locus_tag="ACGI6B_002725"
     CDS             13904..14137
                     /locus_tag="ACGI6B_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9752160.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            14333..14761
                     /locus_tag="ACGI6B_002726"
     CDS             14333..14761
                     /locus_tag="ACGI6B_002726"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFG9752161.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(15215..15985)
                     /locus_tag="ACGI6B_002727"
     CDS             complement(15215..15985)
                     /locus_tag="ACGI6B_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752162.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            16309..16854
                     /locus_tag="ACGI6B_002728"
     CDS             16309..16854
                     /locus_tag="ACGI6B_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFG9752163.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            16886..18022
                     /locus_tag="ACGI6B_002729"
     CDS             16886..18022
                     /locus_tag="ACGI6B_002729"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFG9752164.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            18026..19417
                     /locus_tag="ACGI6B_002730"
     CDS             18026..19417
                     /locus_tag="ACGI6B_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFG9752165.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            19493..20368
                     /gene="dpsA"
                     /locus_tag="ACGI6B_002731"
     CDS             19493..20368
                     /gene="dpsA"
                     /locus_tag="ACGI6B_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HFG9752166.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            20371..20961
                     /locus_tag="ACGI6B_002732"
     CDS             20371..20961
                     /locus_tag="ACGI6B_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HFG9752167.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(21168..21368)
                     /locus_tag="ACGI6B_002733"
     CDS             complement(21168..21368)
                     /locus_tag="ACGI6B_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752168.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         8414 a         2215 c         3612 g         7197 t
ORIGIN      
        1 tgataaaaat ttctttaaat aaaaataatg tcaattataa agtaataaaa gtttagatat
       61 aaaaaaattt gtgtatatac tatatgtagt atataataga taaagcactg actatatgta
      121 gtgcttttgt ttttgaaaaa tacttattgc atttctataa ttatagatta attttgggat
      181 ttcaactgat aagtaccaaa agaaataata agcaaattaa gtgaaattat ggcacaagat
      241 tatgttaaaa ggacggtaaa taatgaattg gatagagctt aacaatcaag tgataattaa
      301 aaatgaacaa ggcaagtacc aattggaaaa ggataaggaa gccttaagta gttacataga
      361 agaatttata cagccaaagt tgagaaaatt taataaccta gaagaaagat tgaagtattt
      421 aatagatgaa ggttattatt caaaagaagt aataaatcag tatagcatag atgttattaa
      481 aaatatatat gatatgattg ataaacataa gtttgaattt caatcatata tgagtgcaaa
      541 taagttttat caaaattatg cattaaagag caatgatgga aaggaaatat tagaaacata
      601 taatgataaa gtgttaattg tagctttgac actaggaaat ggcgataaag atttagcttt
      661 aaacttggca gataaaatag taaaacaaga atttcaacca gctacaccta cttttttaaa
      721 tgctggaaga aaaagggctg gagaaatggt atcatgtttt ttactatctg tagaagatag
      781 tacagaaggt atatcgtatg ctatatcttc atcaaatcac ttatcaaaga ttggtggagg
      841 agttgcacta aatttatcta aacttagagc atctggcgaa tctatcaaag atatagaagg
      901 tgcagctgga ggtgttgttg gagtagctaa aatgttagaa caatcattta gttactttaa
      961 tcaaatggga gctagacaag gttctggtgc ggtgtacctt actgtattcc atcctgattt
     1021 tgaattgttg atggatacta aaaagataaa tgcagatgaa aaaataaggc ttgctacatt
     1081 atcattagga gctataatac cagataaatt tatggagttg gcagaaaaaa atgaagtagc
     1141 atatgcattt tatcctcata ctgtgtataa gaaatatgga gtttctcttg atgagataga
     1201 aatggataag tggtatgata aactggttaa tgatagtgat ataagaaaaa aagaaataaa
     1261 tcctaggcag atgttgacaa aaatagcaca gatgcaacaa gaaagtggat atccatatgt
     1321 agtgtacata gatacagcta atagagaaca tacattaaaa gatgttggaa ttattaaaat
     1381 gtctaactta tgttgtgaga tatttcaata tcaaacacct tcagaaatag aggggtatgg
     1441 aggaaaaaat gaatggggac aagatataag ttgtaaccta ggttctttaa acatagccaa
     1501 tgtaatggat aataaaacta tagaaagtac agttgaaaca gcaataagag cactgtcatt
     1561 tgtagcagat agcacagata taaaaccagt gccaacggta agcaatagta acagcaagtc
     1621 tcattctata ggattaggag ctatgaattt acatggatac ttagtaagag aaaatatttt
     1681 atatacttca gatgatgcta tagatttttc taatgtattt tttgcaatgg ttagatacta
     1741 ttcaattaaa gcttctatga aaatagctat agaaagaaat caaacttttg aaggatttga
     1801 taaatcagaa tatgttaagg gaagaaatag caaggtttta tcaaaatact atgaacaatc
     1861 ttatctacct aagtcagaaa aggtaagagc attatttgaa gggatttata taccaacaaa
     1921 agaagactgg acaaaattat tggatgaggt aaaagaaaaa gggatatata atgcatattt
     1981 aatggcagta gcaccaactc aaagcattag ttatgttcaa aatgctacat caagtataat
     2041 gccaattact gagccagtag aagtaagaac atatggagac tctactacaa tatatccaat
     2101 gccattttta acaaatgata atatgttgta ttatcaatca gcatacagga tggatatgag
     2161 aaaggtcata gatttagtgg caacagttca aaaccatgtt gaccaaggta tatcaacaac
     2221 actgtttgtt acagatgaaa agacaactag agatatagct agacattaca tatatgccta
     2281 taaaaaaggt ttgaaaagtt tatattacac aagaacaaag atgacaagag atactcatga
     2341 gtgtctagtg tgttcagtat agggagagaa atatatgaca tttaatttac ataaaataca
     2401 taatgcagtt aactggaata gagaagaaga tggatttaca caagcttttt gggaacaaaa
     2461 tgtaaaacaa ttttggcttc ctgaagaaat atctgtttct aaagacataa aggtatggaa
     2521 tgaactaagt aataaagaaa aggaattata taaaaaagta ttgggtggtt taacattact
     2581 ggatacaaaa caggggaata atggaatacc atctatgatg agtttaacag aaaacctaca
     2641 aagaaaagct gtcttgtctt ttatggggac tatggaagaa atacatgcaa aaagttattc
     2701 ttctatattt atgactctac tttctaattt agaaatagat gaattatttg aatggataga
     2761 aacagaacca acattacaaa gaaaggctga tttagtttta gctcaatacg aaaatactac
     2821 aaatcaagag ggattatacc tatcaatggt tacaagtgta tttttagaga gtttcttgtt
     2881 ttattcagga ttcttttatc cattgtatct atctggtcaa ggaaagatgg tagctagtgg
     2941 agaaataata tcgcttattc ttagagatga gtctttacat ggtaagtata taggattgct
     3001 agcacaagaa atatacgatt catttgataa aatggataag gagatgttag aagagaagat
     3061 gtattctata ctttatagcc ttatggagaa tgagatagag tatacaaatg ttatatacag
     3121 agaaagtggg cttgaaaaag aggttgtgaa ctttttaaaa tacaatgcga atagagcttt
     3181 agaaaatcta gggtttgaga agctatatac tgtagatgct ataaatccaa ttgtattaaa
     3241 tggtcttagc actgaaacca aaacacatga ttttttttca actaagggta atggatacca
     3301 aaaaggagtg tatgaggaat tagaggatga agattttata atataattta taattatgac
     3361 acactgttgt cacttttagc gtgataatat caatgtataa aatatatatg gaggtatttt
     3421 tatgaaatat gaatggagaa aaagagataa agagttatat ttacctaaaa agaatcctgt
     3481 aatagttgaa ataccagaac agaaatttat catgttaaaa gggagtggag acccaaattc
     3541 taaagaattt actgaagcaa taggagtact ttattctttg gcatatgcaa taaaaaccat
     3601 gcctaaaaaa ggaattgtac cagaagggta ttttgactat acggtttttc ctttagaagg
     3661 tatatgggat aagggagaaa aaagtaaagg cttagatgta cttattaaag aagatttaat
     3721 ttacactata atgatacgtc agccagattt tgttacacaa gacctgttgg ataaagctac
     3781 tgatatagta aagaagaaaa aacctcataa tcttcttgaa aaagttagat ttgggtctat
     3841 taatgaaggg ctatgtgttc aaatgcttca tataggttca tatgataatg aaccagaaag
     3901 ctttgacatt atgaacgaat tttgcaaaaa aaataaactt ataagaaaat cttacacaca
     3961 tcgtgaaatt tatataagtg atgctagaaa agtatcgcct gaatcattga agacggtttt
     4021 acgttttgaa gtagaaaggg aagtataaat attttgaata atttataaga gttataaaaa
     4081 gtgaagtctg ttgagacttc attttttgat gcaattttaa aattaaattt gatgtttcat
     4141 aatattttaa aaactttcta cttttattag tgtgaataac tgttgctatt aaataataat
     4201 actaattata taagtattat tatataatat ttatagatta ttatataaat agattattag
     4261 taagatgtat gcattattaa ttaaaataaa ttacatatac tatttaatat ttcatttaaa
     4321 atattaaaaa atgtagataa aaattgtttt attaagaaaa aattaatata ttgacaaaat
     4381 ttaagtgctc atttaaacta ttaattgata ataatattca atattaaata ataggaggcg
     4441 ttatgaataa atatcttcta ataaatccag tggcagaaag aatgtatgga gaaaagtttc
     4501 accttattaa agcaaatttg ataaaaaagg ggtacataat agcagaatgt gaaccacagt
     4561 tagaatatgt aaaaaagcag tacaaagagt atacaaaaaa gactgaaaat acaatgttgg
     4621 attgtagatg tccagaaagt ataagtcttt taaaaagaaa taatttgata aatgattttg
     4681 aagtacctat gatagaacca atattagtaa ggacttgtag ggttttatac gataaatata
     4741 taaaaagtaa agatgatatg ttggtagtaa cctgtccatg tacacaatta agagactttg
     4801 caagtgaaaa gtttaaggat aagggtaatg ctatgtttta tacatggaaa gagtttgctg
     4861 aacaagaagg tgtaaaatca cttggaaaaa tagatgaatc accaattcca ttaggatatt
     4921 tcaaagatac ttttgaaaaa gttcttgaag tatcaagtga agacgaaata ttaagtgaaa
     4981 tacaaaaaat aagaaatggc tttgaagata agtatgacat agtagaaatg ttttattgta
     5041 aagatggttg taataatgga gatggtttgt aaatggtcaa taaatttaat aataagtttt
     5101 taaggctagt aaagagtgtt ttgatatttt ttataattct tttattatgg aaaattacaa
     5161 attatcttgg tatttggagt gattatatat taccatcacc tgaaaaggta tatagtacat
     5221 ttttaaatat gataagtgat ggttcaatat ttattaatgt atatgcaagt atgaaaagag
     5281 ttttgatagg ttttgcaata agtactgcta taggtgtgcc tttagggata ttctttggaa
     5341 tttatagtgg agtatacgaa tactttaaat ctctaataaa ttttttaagg aatacacctc
     5401 cccttgcttt aataccaatg cttatattat ggtttggtat aggagaggaa tcaaaaatta
     5461 taattatagt tttagcatcc tttttcccaa tctttacaag cacattgaaa gggattaaaa
     5521 attgtgactc aaaactgatt gaagtgggta gagtatttga gttttcaaag cttcagataa
     5581 tatttaaaat aataatacca aatgccattc ttgatattgc tgttgggtta aagttagctc
     5641 ttggatatag ctttagagcc attattgggg cagaacttgt agcagcttca tctggtcttg
     5701 gatacttgat atctgatgga aaagaaatgt ctagaacaga tgttgttatt gtaggtataa
     5761 tcgtaattgg acttttagga ataattacag attacatatt ttcgattatt gtgaagaagg
     5821 taagtaaggg aaagatggtg gaagcatatg aataatttag tatgtgactc tggatatata
     5881 attagaaatt tatataaaag ttataaagtt gacaatgaag aacatgtagt tcttagtgat
     5941 atctctttaa acatagatag aaaccatata acagtaattt taggtgaaag tggttgtgga
     6001 aagactacct tgcttagagc aattgcatca cttgaaggta taaatagtgg tgaaattagg
     6061 tttattaatg ataataaaga gtatagacct aatgttggtt ttgtttttca ggagagcaga
     6121 ttaatgcctt ggttaaacgt aagtgaaaat atagcttttc ataatcaaag aaaaaatact
     6181 attttaaaca aggtgcttag aaaatttaaa cacaataaaa gaattagcag taaaattata
     6241 aatgataatt ttagaagctt agaaacaaat gataatttga acaaatcaaa taaatataat
     6301 aggaaaagta atattgatgt agaaaaatat cttaagatga tgaacttgca gaaatttaaa
     6361 aactcttatc caaatgaatt gtcaggaggt atggcgcaga gagtaagcat agctagagct
     6421 ctttctttca atccagatat gttgcttatg gacgagcctt tttcggcatt agattatttt
     6481 acaaggattg atatgcaaaa tgaagttata agaatacata agtcaactaa taaaggcgtg
     6541 atatttgtaa ctcatgatat tgatgaagcg ttaaagatag gcaagaaaat agttgttttc
     6601 acagacgaaa gaaaagtaaa agaatttaat atagaagatg gttataatag agatttaact
     6661 tcagattact atattaaatt aaaaaaagat attttaatga caatgaaatc aaataaaggc
     6721 atttaaagaa attaataata ttttaaggag taaagtaaat gaaattaaaa aagctattga
     6781 taggtataac aatattaaca cttgcaacag catctttagc tggatgtgct aaaaaaaata
     6841 atggagaaaa actttcagaa ataaatttaa cttatgtaaa atcaccatta aatgttcctt
     6901 ctataattca aaaacaggat gatttatttg gaaaggaatt taaaaaggat aatataaaag
     6961 ttaatttcca cgaaattact acaggtccag agcaaacaca agctttagct gctggagaaa
     7021 tagacttctt acatgcacta ggtggtacat cagctttaat agctgcatct aatggagtag
     7081 agttaaagat attaaacact tatagcaggt caccaaaagg atttatgatt ttgactaata
     7141 ataattcaat aaaatcagca gcagatttag taggtaagaa ggtggcaggc ccaaaaggga
     7201 ctatattaca tcaggtattg atttcagcac ttgataaaga aggattaagt atggatgatg
     7261 tggaatttgt taatatgggt attccagagg catcagcagc tctttctgat ggcagcgtag
     7321 atgctgcatt gattgctggt ccagcagctc ttaaagctat gaagtcaggt agtaaacttg
     7381 tagcaaatgg tgaaggttta gtagatggta taatagtaac tgctgttagc acagactttg
     7441 cagagaaaca tccagaaata gttgaaagat ttatgaaggt tgaaaaggaa actttagaat
     7501 atgtaaataa taattttgat gaagctatgg aaaaagtagc taaagaagtt gatttaagtc
     7561 ttgaagaaac taaagaatta tatgcttggt atgattttag tttagatata acagataaag
     7621 acatatcttc gcttgaagat acacaagact tcttaataaa aaataagtta caagagaaaa
     7681 aagtgaacat aaaagaatta atctataatg ctaagtagaa actatgataa aataatacta
     7741 agtaagaaat atagtaaaag tttaagttac cttattaagg taactttttc tattttgaat
     7801 tagtaatttc aatttatttt agaatgatta gttgataata atgattaatt aatcataaaa
     7861 agatactttt ttatagagtt ttattaagat gtctaataat atggttatac ttatgaagtc
     7921 agtaattgtg aagttagtaa tatgattaaa tttatatatt gtaaaaagat tataatatat
     7981 taaaattgga ctgttaatat gatgagtaaa taggtatatt aggaggaatt atatgagtta
     8041 taaagtatat attgtggaag atgatatatc aataagtaca ttacttaaag attatattga
     8101 gaaatatgga tttgaagtga ttgttgaaga aaactttgat gatataatga ttacatttga
     8161 aagttttaag ccacacttag ttctattaga tgtaaattta cctaaatttg atggtttcta
     8221 ctggtgtaga tgtattagac aacagtcgaa tttacctata atttttatat cagctagaga
     8281 tggtaatgta gaccaaatta gagctttaga aagtggagca gatgattata taacaaaacc
     8341 tttttactat gatgtagtaa tggcaaaaat aaagagtcat ataagaaggg tatatggtga
     8401 atattcacct aaaatagacg aaagaatagt ggagctagat ggattaaagt tttatccaga
     8461 gaggttagaa ctagaattaa atggtgagaa tctaattata actaaaaaag aaggtatact
     8521 gattgaatgt cttatcaaga gatacccaaa agtagtaagt agagattatc ttttagaaaa
     8581 gatatgggat gatattgagt ttgtagagga aaatacttta aatgtaaatg ttagcaggat
     8641 aaggaaaaga tttgaggaat taggtataga taatgctgtt cagactgtcc gagggttagg
     8701 atataaatta aataagaatt ggtaaggaga aagtatgaag ttatttttaa aagattataa
     8761 aggttatatt tttatatatt atataggcat agttatgaca attatttact gtaatcttat
     8821 gaagtttatt agttttggag agagtttata tattctttta tttagttctt ttatattgtt
     8881 atgtttttta atatataagt attataaaaa tagagaagta tataatctgt ttgaaaaagg
     8941 agtacattct ttggaggagt catttttgta tttaggaagc tcattcttgg ggagaaatat
     9001 atcagttatt ttaaaacaac aacataaatt gtatcaatca atgatacaag agcataaaaa
     9061 gatacatagt gaacatttaa ctttcatcaa ccaatggatt catcagatga aaacaccaat
     9121 atcagttata agccttcagc ttcaagatta tgagggagaa gaagtaactg aaaatataag
     9181 aaaagaaata gatagattaa ataaagggtt agatatggct atgcattttg caagggcaga
     9241 taattttcag aaagacttta tagttgaaaa agccaaatta aatgaaattg tatctagtat
     9301 tgtaaatgaa gaaaaaaggt tatttataaa aagtggaatg attcctaaga ttcaagtaga
     9361 caaatttact tatgtttata cagatgtaaa atggatgaaa tttataatag gtcaattaat
     9421 tacaaatgga gtgaaatatt ctaaagatta tagtaactac ctaactataa aatctgaaga
     9481 aaataataaa tatgtaattt taagtataat agatgaagga attggaattg tttctaaaga
     9541 tttaaaaagg gtatttgacc cattttttac aggagaaaat ggtcgtaagt ttggagaatc
     9601 tacaggtatg ggtctttatc tagtaaaaaa ggtatgcgat gatttaggtc ataaagtaag
     9661 tataaaatca gaagtaggaa aaggtacaga agtttcaatc tactttaata aagatgtaaa
     9721 tagtttaata aaggtgtaaa tagtttgata aagatgtaaa ttatctgtta caatagggtt
     9781 tttagttaat tgtacttttt tattatacaa tttattaaat gaaatattca tagttaaata
     9841 atcaaaattt aagagcgttt atttgcaaac cttacataga tgtaaggttt gtgtaatgtt
     9901 tgcagatagt taaattttct aatctaaatt acaattaata taagaaataa cataagagaa
     9961 ataacataaa aaaagaaatt ttgtaaaaaa ggaaggatgt ttattgatga gtttagatgt
    10021 tatttttata ttgaaatcag tcataatagc tatagtagag gggcttacag aatttattcc
    10081 agtttcatct acaggtcata tgattttggt tggtaattta atagatttta aggggcaatt
    10141 tgctgaaatg tttgaggttg taatacaact tggggctata ttagcagtag tagtacttta
    10201 ttggaaaaag ataaaagata gcgttataga attttttaaa tttatattta ctggtggaaa
    10261 agaaggaaag attggtttta gatttggaat gaatgtaatt ataggttgta taccatttgc
    10321 gattatagga gttctatttt acgataatat aaaatcattg tttaatttac aatctgtaat
    10381 tataggattt atagtgggtg ggattttatt attagtagta gaaactttgt ttagaaaaaa
    10441 gaatcattcc acagataata tagataaaat tactccaata caagctttaa aggttgggac
    10501 attacaagtt ttgtctgcat ggccaggtat gtcaaggagt gcatctacta ttatgggtgg
    10561 atggatagca ggacttaatt ctcctacagc agctgagttt tctttctttt tagcagtacc
    10621 agctatggtg gcttcttcag gaaaagattt atttgaattt gattattcaa taatgacacc
    10681 aacattgtgg attgctttgg tagttgggtt tatagtagct tttattgtat caataatagt
    10741 tatggaaaaa ttcgtgaatt ttttaaagaa aaaaccaatg agagtgtttg ctgtatacag
    10801 aataataatg ggtgtagtac ttgcagtatt agcttttact aacataatta gtgtatagaa
    10861 aaaatagatt tatcatctat aaaaattaaa ctaagataaa ttataattaa caattatata
    10921 tatgttattt caaaaattaa attaggaagg atagttaaat aatgaaaatt ttaactgtaa
    10981 ataatttaag taaagtatat ggtaaaaaaa taattttcaa cgcattaaat gacataaatt
    11041 ttagtataga agatggagaa tttgtaggaa ttatgggtcc atctggtagt ggaaaaacta
    11101 ctcttttaaa tatgatttct actattgaca agccaactac aggaactatg gaattaaagg
    11161 gcaagaatcc tttattgtta agaggtgagg aacttgcatt acttagaaga agagagttag
    11221 gttttgtatt tcaggatttt aatttattgg atacattaac tataggagaa aatatagtat
    11281 tgccattgac attagataag gtcagtgtaa aagagcaaga tgagagattg aatgaagtat
    11341 ctacaatatt aggtataaaa gatttattag gaaaaagaac ttttgaagta tcaggtggtc
    11401 aagctcagag gactgctatt gctagagcat tgattaacaa tccatcaata cttttagcag
    11461 atgaaccaac aggtaattta gattcaaagt catcaaaggt tgtaatggag ctatttcaaa
    11521 agataaataa agaaaataaa gttactacta tgatggtaac tcatgaccca ttagcagcga
    11581 gctactgtag tagaatctta ttcataaaag atgggtctat atataatgaa atatataagg
    11641 gtagtagtag agagcagttt tatcaagaga taatggatgt acttacatta ttgggaggcg
    11701 ataactaatg aatataaaac agtttgctat taacaatatt tcaagaaata ttaaagcgta
    11761 tttaggatac ttagcaagta ttgttatatc atcttcactg ctattttctt ttattatgtt
    11821 tacaagtcat ccagatttag atgtatctca atttcctgat tatttaaaag aaggtctcaa
    11881 gatgagtaag ataattgcat atctattctt attcttcttt gtgttttatt cagttagtgt
    11941 gtttcttaaa agtagatata aagaatttgg aatactttat attacaggta tatcaaagcg
    12001 ccaagtaatg aaattaatct ttatagaaaa tatgataata aatatagtat cttctgtaat
    12061 tggaataata ataggcttaa tctttgcaaa gatattttta gtgattatgt ctactttttt
    12121 agaattatca ccattaaaat tctatatacc atttaatgcg atattgtcta ctttaatcta
    12181 ttttatgatt ttagctgtat taacatcaat atttacttct tttatagtta aagaaaatca
    12241 ggtgttgaag ttacttaaag gtacaaaaac tccaaaacca gagcctaaaa catcactaat
    12301 gcttgctata ttaagtattt gtttgtttat aatagcgtat tataatgcta ttacctcaac
    12361 cgaccaatat acaacatatc tgcgcttagt tccagttaca tcccttacta tagtagcaac
    12421 ttacttgttc ttttctcaat ttagtgtatt ttttatgaag aagctaaaac taaacaagag
    12481 attttataga aaaaatacaa atatgctttg gatttcgaac ttgatttata aggtaaagga
    12541 taatgctaga atatttttct tgataactat aacttctgca gtagcattta ctgctatagg
    12601 tacagtttat tccttttgga aagatgtaga gcgtcaaatt aatttaatat atcctaacac
    12661 aatatattat tcaacaatga cattgcataa tgaccctaaa aagcctgata gcaaagaaaa
    12721 agacaaggaa agaatgatgt ttatagaaaa taatctaaaa aaagagaatg tagactatac
    12781 aaggactaat ggtgaattta aaactgtatt tcccaagaaa agtgatttta atgtcagaat
    12841 tatgaaagaa tctaagtact tagaaattac agaaactata ggggttaagt ctataagttt
    12901 tgaagataat gaatgtatat cattattatc atcaagttta ccaggaaata ataacgtcaa
    12961 agaaaatatt attgtaggga ataggggttt aaaggtggca aaacaagtgg aagaatgtgt
    13021 tatgccagca tattacaaaa atatgtatgt ggtaaaggat aatttttatg atagtataga
    13081 atcaaaattt atagtggata gatttactgc atttgaagta gaggattcaa gtaaaactat
    13141 agagatatgc agagaatttg aaaataaatt taataatgag tcagggatgc aaccatattt
    13201 gtttttctct aaagaattaa tgataggaac tgggaaatta atgtattcta catttatatt
    13261 tttagcaata tttataggtt taattttctt tgttactaca agtagttttt tatataataa
    13321 attttatatg gattgccaag ttgataagaa aaagtatgaa cagttaaata aattgggtat
    13381 gacatataat gagattaaaa aagcttcaac aattgagatt ggaatagtat ttttgtttcc
    13441 ttatgtagtg gctgttatac attcaatatt tgcacttact gcacttaaaa attcttttga
    13501 tatagaagta gcatcatcag ctgttttggt aatgggaagc ttgttcttgg ttcaaatagt
    13561 ttattttttt attgtaagaa ataattattt aaaagagata aggttaaatt tagtcaattt
    13621 ttagttacta ttattaaatg ataataaaaa gggtttaaaa ttaattaata ttactggaat
    13681 tatagatgtt tattttttca gtaatatttt ttattgtatt aactattaaa attttgtaca
    13741 aatataaaat ataatatggt aaatgtcaaa attataaaaa aattctactt atataaaata
    13801 taaagaggtt atatgtacat atgtgtagaa ataaataata aaaatatcaa ttaatgttat
    13861 tagggggggg ttactcatta gtaaattagc taatacactt agaatgatga tattaattca
    13921 aagcagaggc aaaatgaaat gcaaagaatt atcagaagag ttggaagtaa gtgaaaggca
    13981 aataaaaagt tacaagatgt atttagagca agctggtata tttataaatt caacacctgg
    14041 gatttatggt ggttatgaaa tagataaatg taatagcata tcattaatta aattattaga
    14101 tagtgaagtt tcaattttta tatcaactaa gtggtagata agtagaatat tagcaatatc
    14161 aacgagtatt tagaaactta ttttatatta ataaagcagt atataaaatt gtagaatcaa
    14221 caatagcata tactaaaaca tccaccttat attaactaaa tcatatctaa ataaaataca
    14281 cttacctaca aacattataa aatcaataca aaaatgaggt gaaacaaaat ttatgataaa
    14341 gaaattaaac agtaaagaca taaataaaat catggaaata tgggaaaaaa gtacaatcaa
    14401 agcacatgac tttataagta aagaatactg gcaaaataac tataatactg ttaaaaacga
    14461 atatatacct atatcagata catttgtata tgatgatgga gatgaaataa aaggatttat
    14521 aagcataata gataaaaact ttataggagc tttatttata aagcccaaat accaaaatct
    14581 aggtatcgga ggtaaacttt tagattatgc aactaaaaaa tataaaagtc taagcttagc
    14641 agtatataaa gataataaaa aagcagttgt gttttataat aaaaaaggtt ttaatatagt
    14701 aaaagaacaa gtaaatgaag attcaggatt taaagagtac ataatggaat atagtaaata
    14761 atatgattac attctattaa aaggataatt aatttatttt ctagttgatt ttttaagagt
    14821 aatttttaaa atagactata tgaagataaa gatatttatt atagatgtaa gttaatcaga
    14881 attgatatag ctacaaaaga ggaaaataaa attgtatggt gtatttcaga tttaacaaat
    14941 ttactattta aagataataa gtttgatata atattaaaga tactttcacc tataaactat
    15001 agagtattga aagaatttta aataattatg gaatgctaat aaaggtttgt taaaatttaa
    15061 taattattag attatagaaa attgccatga aattagttca tcaagaacta gtttcatagc
    15121 aattttaatt cttataagtg tatttatttt tacagattta aaaaacttat acagtatcta
    15181 ataatgtaat cttattactt tagcctataa atatttaaat cttaaaatgt tctctttgag
    15241 ataagcttat ttcagagtca caatttccac aacagtaaaa tgttgagaca tctttaaatt
    15301 tttcagtatt ctcatcaaag ttcatttttt tctttgcaat aattcctacc ttagttaaat
    15361 tagaaccaca attattacag ttttgaattt tgcataggtt atatagttta tcaagtgtaa
    15421 tatcttctaa taattcatca caattacaac atctgataga agaaacttca ctacacattt
    15481 caataggttc agcttcttta gaagaaaatt tatatgttat gtcatttgat actttataac
    15541 ctgtatcaaa tatattagaa ttacaacttg gacataagta ctttggaaga tatatatctt
    15601 ttttttcaaa agtatcacta ctaatattac agtttaattc atctaaaatt ggtctttcat
    15661 ctaggttatt acctttattt aagcttagaa gataattata aattttttta tcttcattct
    15721 gttttgatgt atctaacaaa tgctccatta tattagtgta ttttttgttt tttgaaggca
    15781 tgtttaaatc cagataattg ttttcaatag caaagcatac tatattgaaa tagttatatt
    15841 taactgcaaa aaataataac atttcatcaa atactttatc tattattttg tcagaaatac
    15901 ttatccaaaa atctggtgac tctaagtaaa ctttcctact aagttcatct aactcttcat
    15961 ctatattatt ttcattaact ttcatatcat cacttccttt taaatatctt gtgtataaac
    16021 tatttgttac tattttttat ataaaataaa atcttatata aaaatatatc atattataat
    16081 ttacaagtat agtctaaata gagctgtttt taagcaaagt tttataaatg actattaaaa
    16141 tataaagtaa cagtataaag tttacttata aattaaatgt aaacctaaat aattataata
    16201 gttgacaaaa aaacgtcttt attttatact aaaacaaagc tttaatagaa gctttgtata
    16261 tgtacatata aactaaatga aataagacat atgaagggaa ttataaaaat gaaaaatact
    16321 aaagtaaata ttcaagattt aactaaaatg tcaatatgtg tagcattatt gtgtatatca
    16381 tcttacatat atataccatt gccatttact ccagcaggag taacagcaca aacaataatg
    16441 ataaatttaa tagctttgat tttaacacca agacaagcat ttagtacagt tggtgtatat
    16501 ataatgatag gtttaattgg gattccagta tttggtggag gtacatctgg tattggaaag
    16561 ttattaagcc caacaggtgg tttttatttt gggtttttgt ttgcagcact aattattagt
    16621 ttattaaaag gacgaaataa tgatattaag agatatatat taataacaat ttttgttgga
    16681 atgcctatta tatattttat gggaacagta tttatgtgct attttaatca aatgacagta
    16741 gaagcagcac tttttgcagc agtagtacca tttttaattg gagatacaat aaagtcagtt
    16801 attgcatctt ttttaggcac taaactgaac aatgtactca gaagaaatct atgataaatc
    16861 tataatataa ggaagggtgt aatgtatgaa ggaagttttt atattaggag gattgagaag
    16921 tcatataggt ctaaaaaatg gaatttttca atttgttcaa cctgaattac taggagcaag
    16981 tgttttaaaa gatttaatta aaaagtatga aattgacaaa attgatgaga ttatatgtgg
    17041 aaatgcagtt gggacaggag ggaatatagc tagattaatg actttaacag caggagtgtc
    17101 aaatgaagta cctgctttta ctgtggatat gcagtgtgct tctgctatga tgagtataga
    17161 catagctttt tcaaaggtta aatctggtca gtgtgattta attattgcag gtggttttga
    17221 aagtagttct ctgcaaccaa tgcgcacata tcataagaat gataagcgat ataatacaaa
    17281 taatccaaat tatacagttg ctcaattttc tcctgatgat aatagccaaa atagtatgct
    17341 cgaaggtgcg gaaagagtag cagagttata tcaaattgag aaagcagact tggatttttg
    17401 ggtaaaggaa agtcataaaa gagcaaaaga agcaagagag gaaaaaatac ttgaagatat
    17461 tatatctcct ataaataata gtacctatga tgaaggtata cgtgataaga tgagtcaaag
    17521 gttattagat aggatgccat caattttagg aaaagaaact attacaaatg cagcaaattc
    17581 atgtcttatt aatgatggag cttcatttat tataatatgt tctaagaagt atttagaaca
    17641 tgtaaaaaag aagcctaaag caaaaattat taacacatgc acgataggaa cagatgcaac
    17701 cttaagtcca acatcagcga tacaagcaat ggataaactt ttagagatag aaagccttaa
    17761 ttatttagat gtatctgctg ttgaatttaa tgaagcattt gcagttatag atgtgttgtt
    17821 tcaaagaaag tatccagagt taatagatag atataatata tttggtggag ctttagcgta
    17881 tggacatcca tatggtgctt caggtgcaat tattgcatta catcttttaa aagcattaga
    17941 aaagactaag ggtagatatg gaatttgctc tatagctgca gctggtggtc taggttcagc
    18001 tttactgctt gaaagggtgt agattatgaa ctattatgaa cttttaagaa taaaaaaaga
    18061 gaggcataaa aataaaaatt ttttaattat agatggagaa aaatatactt atgaaaatat
    18121 attgagttat agtgaggaac taggaaagca aatttcttta ggtgaaaata tcccaatttt
    18181 aatatattct aaaaatataa tgtttcagtt gattagtttt tttgctatta attattcaaa
    18241 aaatattcct attatttgtc attataatct ttctaaaaaa gtattagatg atattttatt
    18301 aaaaaataat atttcaatca ttatatctga tgaatctatg gacgttattg atttatatga
    18361 tgataacaat aatgaagtag caaaaaaagt taatttttct atttttaatc ctaaatttaa
    18421 tatatatatg tatcaatata agaagcttat aaattattta gataaaagta tttgtatggg
    18481 agctttatct tcaggttcta caagtgttcc aaaggtatta tatagaacat atgagagttg
    18541 ggctggattt tttcctatac aaaatggtat ttttaatatc tctggggaat caatcttgtt
    18601 tgttaatgga acacttagtt ttacagggaa tctaaattct ataatgtcag tattatatga
    18661 aggaggtagt attgtaataa gttcaggtct taattgtaaa tcatggataa ggacaataaa
    18721 acaatataat gtaactaaca tataccttat acctacaaaa cttcaacttt tggttaaaca
    18781 cttaaaagaa ccagtactca aagttgtaag tatttttaca gggtctcaat tattatttga
    18841 agatactgca agaaatctaa aaaaatatat gccaaagtct gaaattattt tatactatgg
    18901 ggcaagtgaa ttaaattata ttacatattt atgctatgat gaactaattg aaaaacctct
    18961 aagtgtaggc agaccatttc caggaataga tatatatatt agagaaggaa aaatttttgt
    19021 gaatacagaa tatgcagtat ataatgcaac caaaccttat tctgtcaatg atattggata
    19081 ttatgataat gatggatact taatatttga aggaagaagt gatgacatta taaatattgg
    19141 aggatttaaa gttagttcta ctaagattga aaatgaagtt aaaaaaattc ctcaaattga
    19201 agatgcaatt gtattgccgt actcagacac tataagagga aatcaaattg tattatttgt
    19261 aataactata gacaaaatta cgaagaaaga tttgttgatt aaaatgaaag ataatctaat
    19321 gaaaaatgaa atcccaaaga aaattatctt tttaaattca tttccatata cctcttctga
    19381 aaagatagat agattagcat tattaaaaat gttataagta taatatttag taattttagt
    19441 gaaccaatgt attgataaaa acatattgta tatagtaaag gaggcttaca atatgtcaaa
    19501 tcaatacgat ataactgtta ttgggggaga cctgcgacaa gtgtacatgg taaaaaaact
    19561 aattaataaa gggtattctg tagtcactta tgggatatca aatgaaatta taaatggaat
    19621 cgttggaaaa tcaacttcac tagctgaagc actatccaaa agttccatta ttttatgtcc
    19681 aattccattt acaaagaatc aagttaatat tgaaaatacg gatgattgtc ttgatgctac
    19741 tattgataac ttgctaatta atttatctcc taatcaaata ttatttggag gtaacatacc
    19801 aactaaagtt tgtaaatttt gcgagcaaca ctctattcag atttatgatt ttatgaaaat
    19861 gaatgatgtt gctattttaa atgcagttgc tacagctgaa ggggctatag cagaagctat
    19921 taaaagaagt ataatcaata tacatcaaag taactgttta gtattaggtt atggaagatg
    19981 tgcacaaata cttgcaaaaa aattatacgc tttggatgca aatgtatgtg ttggcgcaag
    20041 aaataatgat gcaagaagta cggctaatgc atttggatat tccagtattt ctttaagtca
    20101 acttgatgaa aaacttttac aatttgatta tatttttaac accataccag agtgtatttt
    20161 aacgaaagaa aggttagaaa aagtatcaca agaagtaact attatagata tagcttcttc
    20221 tcctggagga gtagattatt ctgttgttaa agaagcaggg attaatgcga ctctttgcct
    20281 tgggcttcct ggtaaatatt caccaaagac atctggtgaa attcttgtta acgctataga
    20341 aaatattata aatgaaagaa gggattagaa atgagacttg aaggacttac aattggagta
    20401 ggatttactg gttcgttttg tacttatgat aaaatattta tagagttaga gaatttggtt
    20461 aaagaaggag ctaatgtaca cactatattt tcagatgtat cacaaaatat cgattgtaga
    20521 tttggaaatt ctgaagagtt tatgaaaaaa gcatatgaac ttacaggaaa taaacctata
    20581 gttacgattg aagaggcaga gccatttgga cctaaaggaa ttgctgatat tataataata
    20641 gctccttgta ctggaaatac tgctgcaaaa ttagcaaatg gtattacaga ttcaccagta
    20701 ttaatggcag caaaaggtca tcttcgtaat gataaaccac tagtaatatc aatatcaacg
    20761 aatgatgctc taagctttaa tttcaaaaat attggcattc tccttaattc taaaaatata
    20821 tattttgtac cttttggtca agataattgc aaagcaaagc ctaattctat gattgcacat
    20881 actgagctaa taattccaac tatagaactt gctctagaga acaaacagct acagcctgtt
    20941 ataaaaagtc cacatcaata gaattggttc ttataatatt atgtattaga tacaatacta
    21001 gcaaagaggc tgtctcaaaa tagagagaag cctctttttc ttgttgaaat gatgaaaata
    21061 tatttaattt catacaaaaa aatgcctcat ggactaaaaa gtctattttg agctaatctc
    21121 cgtaattttt gctatattaa aattatttac ttaatccaat ttttaaatta gaatttatat
    21181 cgttttgact cttcaaatat caatctaact gttgttgata caaaagaaga ggacataatt
    21241 aaaacaaata ctgatagtcc tctaataaac aaattatcta cataataagt tactattcca
    21301 atacctccaa tcgttaaaat tacacttata atcaaagaaa aaacgctaat tgtttttaat
    21361 ttttccatgc ttatcctcct tcttataaac ttcaaattta tctatataat attaaatata
    21421 ttttatttca tattatat
//
LOCUS       DAYAOY010000073        20781 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYAOY010000073 DAYAOY010000000
VERSION     DAYAOY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.073"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(14..421)
                     /gene="tnpA"
                     /locus_tag="ACGI6B_002734"
     CDS             complement(14..421)
                     /gene="tnpA"
                     /locus_tag="ACGI6B_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFG9752169.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(987..1199)
                     /locus_tag="ACGI6B_002735"
     CDS             complement(987..1199)
                     /locus_tag="ACGI6B_002735"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFG9752170.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLD"
     gene            complement(1292..1849)
                     /locus_tag="ACGI6B_002736"
     CDS             complement(1292..1849)
                     /locus_tag="ACGI6B_002736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752171.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHISSR"
     gene            complement(2208..4118)
                     /gene="phnW"
                     /locus_tag="ACGI6B_002737"
     CDS             complement(2208..4118)
                     /gene="phnW"
                     /locus_tag="ACGI6B_002737"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFG9752172.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(4397..5182)
                     /locus_tag="ACGI6B_002738"
     CDS             complement(4397..5182)
                     /locus_tag="ACGI6B_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9752173.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(5399..5629)
                     /gene="dltC"
                     /locus_tag="ACGI6B_002739"
     CDS             complement(5399..5629)
                     /gene="dltC"
                     /locus_tag="ACGI6B_002739"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFG9752174.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(5648..6796)
                     /gene="dltB"
                     /locus_tag="ACGI6B_002740"
     CDS             complement(5648..6796)
                     /gene="dltB"
                     /locus_tag="ACGI6B_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFG9752175.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(6796..8310)
                     /locus_tag="ACGI6B_002741"
     CDS             complement(6796..8310)
                     /locus_tag="ACGI6B_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HFG9752176.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(8431..9621)
                     /gene="dltD"
                     /locus_tag="ACGI6B_002742"
     CDS             complement(8431..9621)
                     /gene="dltD"
                     /locus_tag="ACGI6B_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFG9752177.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(9909..10928)
                     /locus_tag="ACGI6B_002743"
     CDS             complement(9909..10928)
                     /locus_tag="ACGI6B_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9752178.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(11104..11643)
                     /locus_tag="ACGI6B_002744"
     CDS             complement(11104..11643)
                     /locus_tag="ACGI6B_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFG9752179.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(11744..12424)
                     /locus_tag="ACGI6B_002745"
     CDS             complement(11744..12424)
                     /locus_tag="ACGI6B_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9752180.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(12453..13742)
                     /locus_tag="ACGI6B_002746"
     CDS             complement(12453..13742)
                     /locus_tag="ACGI6B_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFG9752181.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(14191..15792)
                     /locus_tag="ACGI6B_002747"
     CDS             complement(14191..15792)
                     /locus_tag="ACGI6B_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752182.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(15903..16580)
                     /locus_tag="ACGI6B_002748"
     CDS             complement(15903..16580)
                     /locus_tag="ACGI6B_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752183.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(16593..17288)
                     /locus_tag="ACGI6B_002749"
     CDS             complement(16593..17288)
                     /locus_tag="ACGI6B_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFG9752184.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(17340..18539)
                     /locus_tag="ACGI6B_002750"
     CDS             complement(17340..18539)
                     /locus_tag="ACGI6B_002750"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFG9752185.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(18800..>20781)
                     /locus_tag="ACGI6B_002751"
     CDS             complement(18800..>20781)
                     /locus_tag="ACGI6B_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9752186.1"
                     /translation="LENIFGEFCNIDNLVYVNRINIELSSYELVVCSDNDIKEYIHNN
                     IDKNIPIVIVHRTINIENINQIISIENDSDVMVIDAYKESADETAKIIRKLGLIHINL
                     IPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGDKVIDINTVIEIFTKLNISIDKL
                     HIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGIASVDKLGKFIYCNKVFSNLVGI
                     DQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNLNNKKLIINKVNVYENNERIKSI
                     ISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVGESKIIKEKINIARKIATTDFSV
                     LILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSLSDTLIESELFGYDEGSFTGAIK
                     GGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQEKEVMRMGGSKIIPIDVRIIAA
                     TNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERKEDIPLISKYFLDEINSNKCFTE
                     ESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDYEHLPEQFRFEKNNTLVNENFDS
                     IILDFKQSNFFEESICILTSIETWNNKNILLGRNKLQEILKEKDIVLSVDQIRKRIDK
                     LKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
BASE COUNT         6588 a         3805 c         2032 g         8356 t
ORIGIN      
        1 cactactgcc acctcacttt acttgttttt tctgattatt aatatatttt cttatctgtt
       61 cttctgtatt ttctgataca gtagcaacga aataactagg attccaaaga tgtcctcccc
      121 ataatttctt tttcaactgt tctccatgtt ttttcattaa cagccttgct gatacacctt
      181 ttaatgcttt tattatatct ggtatatagt gttgtggtga acaatttatt agtaaatgta
      241 tgtgatcttt atcactatta cattctaata tttgaaattt attatcttca gctattttgt
      301 ttaatatatt aattaaatct tcttcaattt catttgttaa tactggatgt ctatatttta
      361 cacaccatac tatatggtat tgtactaaat acacataccc tctaccatga gtcacttcca
      421 tttttcatca cccaacataa tattaacata gtaattactt aaaacaagta ttcttaaggt
      481 ttttatattt atttttaaat aacatacgta agaaacgcaa atcttaactt ttatcatttt
      541 tgaggttgtc gttcacataa gttcgctact acctatgcag ttctcttatg aacttcttac
      601 actttcatgc aagcacagac tatatcttat ccttcggcat tatccgttaa ggtctaccca
      661 cttccactac cgatagcttg tagtgtactt ccctcaagag gaatagtcgt tgaagttttt
      721 cctattcgga gcttacctgc tgattgccca ttttaaagta cttaggattt aaccttatac
      781 catctaacca attttttcta ctttcgtaac tttcacactt aggtttattt catccttatg
      841 ttttagtttg gttagcttta ggggtttcca gcaattcaag tagtattgga taggttctat
      901 cctatctcta catataagtt tccctatatg ctgactattt tagctactaa ctcatgacta
      961 aagtcacaag tgtgcatagc tattttttaa tcaagagaag tctcttcttt catagacaat
     1021 attaactcta ttctttctag tattttatct cttggaaaga aagtctcttg acctgtaaaa
     1081 gctgattttt ttataaacca ttcctctggt attatgtttt ttcttttcca tctatataat
     1141 tgtccataag atatattagt tttgctcaat aaatctttct tagatattaa ttcttccata
     1201 gctcttctag cccaccttta tactgttttt tacagtagaa ttatctgaaa ctgataaatt
     1261 atcactgtat tcaatatttc caacatcata attatctact agagatatgc tttttttcta
     1321 gcatacgaaa ttttttgcaa taatctccat gccccttttt tatatttata ctacttgctc
     1381 caacttcatt aaaattagaa gttccattta aactaatatt aacttcttca caattcaaaa
     1441 atccattaca ctccaagatt ccacttaaat tgatattttc acaatctata ttctcatttg
     1501 cttttaaata tccttctcca tgaataatat ccgattttat acttccttct acattaataa
     1561 ttccactagt atcaatttca acagaattaa tatttcctgt acatctaaga agtcccgata
     1621 tctcaacttt tgatgccagt atgtttcctt ctactttact taaaccagat gtaaaaaatt
     1681 cattacattc tgttgtccca aaaacttttg ctgaaccaga agaatcaaat ttactacatt
     1741 ttacacttcc ttctattttg cctgttcctg atatattaac attatcaaaa attccacctt
     1801 tacattttcc aacaccatca aatcttagtg aacttaattc ttcattcata atgatatcct
     1861 ttctttaccg tattttatct ttaaacctaa tataacaaaa cattgttacg ttgtcaagta
     1921 tgataataat acttaataaa gcaactatca tttattttct ataaataggc ataaaaataa
     1981 tagggaaatg ctttatttaa actataaatc taatttctta gattgtggtt taaataaaat
     2041 atatccctat tactcaatgt ttatatattc attgtataaa cttctaaaat ataagtcttt
     2101 aaattctact gtcaatactt tttatacact gtttgtgcta tcatctcaca cacctaaagg
     2161 agtaggcttt gcgcactatt ctgtaaccta caagacacta tcttcttcta tctattgata
     2221 aatttttcta ttacatcagc tagtctctcc atatcagttg gatagacttc acctatgcta
     2281 cctattctaa atgtatctat atcagtcaat ttacctggat agataacaaa tccattatct
     2341 tttaaataag tataaaattc cataaattca aactttgcat ttttaggata taagaaagtt
     2401 gttattacag gtgattgagc attatcattc accaaagtat caaagcctaa agattttaat
     2461 cttgaagcta ttgtaaactg attttcttta tatctagcat atcttttttc aacagaacct
     2521 tcttcttcca attctaataa agcttgataa aaagctctta caacatgtgt aggtgaagta
     2581 aatctccatt taccattatt tttctccata gtctcccatt gcgcatacac atcaagagat
     2641 aatgatttag ctataccttt acatttagat aatttctctt tattagctat tataaaacca
     2701 aatccaggta ctccttgtat acatttgtta gaactactaa ctaaaaaatc tatattaaaa
     2761 tcttcaacat caatctcaat tcccccaaaa ctactcatag catcaactat ataaattttg
     2821 ttatattctt ttgcaagttt ccctacctct tgtataggat ttaatctacc tgtagtagtt
     2881 tcacagtgaa ccattgatat atgtgttata tccttgtttt cttttaataa attttctact
     2941 acatttaaat ccactgcttc tatatcttta aatgtgcagt caacaaattg tatgttaaga
     3001 taattacata tatctttcat tctctttcca tatgctccat ttgcaattac aagaagttta
     3061 ccatcttttg aaatagtact tcctattata gcttctactg aaaaagtccc acttccttgc
     3121 attaatacac ttgtgtattt atctgtattt tgggttgcta gagatactaa tcttcttcta
     3181 acatcttgta ctaggttatt atattcaaca tcccaagtac accaatcttt taacatagat
     3241 gctcttactg atttagtagt agatagtggt cctggtgtaa gtaatatgta atcattttct
     3301 ggtacaaaat ctgatttaat agtttcattc tctattttta ttagtatatc ttctaactca
     3361 gccatagttt ctattacaaa atgtgcacct gcctctttaa atttctttga aactatactc
     3421 atcttagctt ttaattcttc cttatccatg ttctctactt cttcttgtgt aagccctaac
     3481 tcacttgaac cttttattac agcaacactc cacattcctg catttacgcc ttcttttaca
     3541 tcacttattg tatctcctac ttttaccata gaactcatag gagacacccc taaagcttca
     3601 gcatttttat agcacatcca aggatacggt cttccttgag atacttctga tggtgttaca
     3661 agaaaatcgg gtgagtatcc tttctttgct gcatttggtt caactatgtt catcatctct
     3721 cttgtataac ccgttgtaga acctattttt aatccatttt ttctcaactt ttcaatagtt
     3781 tcaacaacat gaggaattgg agtcgtatac tcttctaatg tctcaaataa cattggctca
     3841 aattcagcat aaagttcatt tacatcatct tcagttggca ctttaccaaa cttatctgac
     3901 caaatattct ttattctatc catttcacac atttctctta tatggtctat ttttaatttt
     3961 cccataggtt ttcttgcttc ttccattgtt acatctatac ctcttctttt aaatatctct
     4021 ataaatacat taagaggtgc aaaacatcca taatctactg ttgttccagc ccaatcgaat
     4081 actactgctt ttattttttc tccatatatc tttttcatat ttatagcctc catagattct
     4141 ttttagaatt tttgtgtttt taattgtttt tacttttaaa gtataacttt catatgttaa
     4201 ttatacatca tatttaggtt aaatatatat taaatttctg tttttagttg tatttagttg
     4261 tttttatttt gtatcatatt cccataaaat cctaagttac aatctaaaat ttactttcta
     4321 atcttaatct atgaactgac tatattttat gaatttagtt gtattgagca tagttgtatt
     4381 atataaaact aagggtttat tttgcttcta tccatgttat atttatctca tttaacttat
     4441 ccttccattc attcggaggt tctacatcac aaataacaaa atctaattcc tctaaatctg
     4501 ctatcttata catactagca attcctattt tagaactatc acataataca aaactttcct
     4561 ttgaattttt tatatattct tttgacaaca atgccttatt ttggtctata ctactaattc
     4621 caaaactatc aagtatacct tcactagata taaaagcttt atctacgtat aagtttttca
     4681 tagtatctat tgacattgga catgctgtcc tttggtgttt taaatttact tttccaccaa
     4741 taaatataac ttctccatca aacaaacctt gattctcata agaaatcaat tttgatagaa
     4801 ctggataaga gtttgtaata atttttaaat tgtttacaga tattatataa tctacaatct
     4861 gcaaattggt agtaccttca tctatgataa ttgactcatt gtcatttaca aacttagctg
     4921 catatttagc aatactttgc tttttatcta ggttagtatt gtttctcttt atatgttctg
     4981 gttcaatcac tccataattt atttttatag caccaccata tacctttttt aatttatttt
     5041 cattttctaa ttcttccaaa tatcttctta ttgtttctga tgatacttct aatcttttta
     5101 ctaattcttt agtgtttacc ttcccttttg aatctaataa tttaagtatt tctttttttc
     5161 tatctatcga atttaaagac attcattttc ctcctaaata taatatattt ttctcctata
     5221 atattacaat gatacctaag atttatgaaa tcatatttat gtattaaatt ttttacttaa
     5281 ttaaagttat tttcaaaaga ctttgtatta tcattaaaaa agtccatcaa atattagtat
     5341 cagatggact ttgtaaaatt ttattttata aattaatatt taaagtctaa ctactaattt
     5401 attttcttgt ctccaagaat tttactaaat tatttacagt agacatatct tttctctcta
     5461 aatcagttgg ttgaagctct atccctaatc tattttcaat ttcaagtaaa acttcaatta
     5521 tagctaatga gtctaaaagt tccgtctcaa ataaattaag gtctaaatct tctcttatct
     5581 catcagtccc taatacatct tcaaatattt ctataactgt ttcttgcata aattttcctc
     5641 cttaaaatta agctaaataa cctgagaata tcagcaatcc aaaacacaca atatggaatg
     5701 taatgaaaat ctgcactctc tcgaaccatt tatctttttt atgtttttta taaaatttag
     5761 attttcgctg atatatatca gttaacacaa gtgccaatcc ttggtataca ccataagcaa
     5821 cataatacca agttagacca tgccaaaatc ccattgttat catggttatc atctgagcca
     5881 catgagcagc agtagtcctt ttcttaaatc ttttctttct cattgatgac ataacaaatc
     5941 tagaaaaaat ataatctcca aaccatcttg aaagacttat atgccatctt gtccagaact
     6001 ctttcatatc tttactgata aatggcttgt caaagtttat aggtacttgt attccaaata
     6061 tgtatcctgt acctatagca aataaactat atcctgcaaa atcaaagaac aggtataaac
     6121 tatatgcgta catataactt aaaatattta taaaactcat atctttaggt attcttgata
     6181 cccaatatgt atttatcaaa aatgctataa caaacttata acctactccc atgacaatat
     6241 tttttattcc tggcagtaag tattcttcta tatactcttt tctagagatt tgtttttcca
     6301 agtcctgttc aaatcttctt gacctatcaa taggtccaga acttaaagtt ggaaaaaata
     6361 acataaaata aagcatcttt gaaattttaa cttcttttat agctccatca taaatttcaa
     6421 taaccatttg tatagttctg aaatttaagt atgatattcc aataaatcca attattccaa
     6481 aagaagtaac tggagatatt ttatttatta ttattgggag cattgatgca aacaagaatc
     6541 cccaatatat gtatttattt ttagtttttc ttcttacata ttcatatcct ttaactatta
     6601 ttacttcaag aataataaat attaccaagt actttagttg gacatcaatt cctactatta
     6661 agaatatcat aaacaaactt gccaacatac cataatactt tatattcttg cccattagac
     6721 ctaaaattac tgctggtatt gatgttaata ataatatata aagataaaaa taatctccat
     6781 attgtgaaaa aatcattaaa tttcctccat aagtttcttt ctgtctattt taccattgat
     6841 atttgttgga aactcagaga ttattgaaat atttcttgga atcatatatg aaggtatata
     6901 ctttccaagt tcctttttta ttatcatccc atttttaata ttacttaaat cattcttttc
     6961 atttaactca acaatacctt ttaagtaagc aattttttcg tccttatata ctggtaaaac
     7021 tactgcattt tttacattat gtacttttct caaattattt tctatatctt ctatttcaat
     7081 tctaaatcca tttaatttaa tctgaaaatc ttttcttcca caataatata tatttccatc
     7141 taaaagatat cccatatctc cagttttata agctctccac tttacaccat ctatttcatc
     7201 atagaaaaat acttcatcag tcttttcttt attattgaag taacctttag aaactgatgg
     7261 tccaattatt ataatttcac ccttttcatt ttctttaagc tcatttccat cttcatcaag
     7321 tatttttatt ttacaatttg acatagggta tccaactgga agactttttt catcatctat
     7381 agccttttga gtcatatcat ttacacttac accaacagta gcctcggttg gtccatatcc
     7441 attgataatt cttgtgtttg gaaatctact cataagttct ttagtaagat tttttgaaag
     7501 tgcttctcct atgaatatca ttgactcaag gttaggaagc attttactat taaactcttt
     7561 ttcagttata catactcctg caaaagaagg agttgacacc catacagcta tatctgagat
     7621 actaaattgt ctaaatagtt ctttatagtc tgctaataca tcttttgata ttgaaaataa
     7681 tgtagctcca tggataagac cagggtatat tgttgtaact gaaaggtcaa aagaatatgc
     7741 tggttggttc attattactt tttcacttcc atctatgttt agatatgggc ttatccaatc
     7801 tgaaaagcta tctaaattgt ttgaacttat ttgaacccct tttggctttc ctgtacttcc
     7861 tgaagtaaat agtatatatg cattttcatc atcttttacc cagttttctt tatctaatga
     7921 ttttccctga tattcattta ttatataatt taatttatcc atatcaatta cattgatatc
     7981 tccaaaatct ttttcatcac taaagttaaa taataccttt ggttttatct ctttagtgac
     8041 ttcaaaaact ctatcaattg gaaaactaat atcaagaggc acatacgctc tacctgattt
     8101 taaagctcct atcatacatg ccattatcat gttttcttta tttccataaa ttactatagg
     8161 agtatcttct tctttgtata catcctttag aaatactgat atagcatctg aatattcatt
     8221 taaatcttta taagaaagtt tatctccatt acacattagt gctgttctat ctgtatttga
     8281 atattttttt attccttcaa taattttcat ataagaatgc tcctttaaaa tccgttataa
     8341 ataaatggca attctttaaa tggagtaaat gttagaactc ctatcgccaa tattataacc
     8401 atagttaaac aaatactagt taacaaatct ttattgttct ttaaatattt taaataatct
     8461 ctcacagaca tctacccatc ctttcgtacc aagatgcatt acatccctta aataatactt
     8521 atctgaccct ttatctttaa ggtttagaac ttcaaagcct ttgctttctg ctattttttg
     8581 tgctttatcg tagtactgat ttctttcttt ttcagaaata ccagtcaaat tatagaattt
     8641 atccatactt gggattaata caacaactgg cttaatacct aagtcagtac atacatttag
     8701 catcagttta taattttcaa actccttaga tgttaataaa tttacatctt tgtctcttcc
     8761 tttgtattgc tctataccat ctttaaaatg ctctttataa tagtacttat caatatttag
     8821 tggattttta ccaactcttt ttttagcatc ttcaatggct ttctttctct catatgacca
     8881 atttattggt ctttttcttt ttgttgctct ctttttatct agcttaatta atcgtttata
     8941 aagaataccc ttttctttaa gagctataca atactttctt ccttgaaaat aaggctctaa
     9001 aagcaccttt tcagcttttt caggaaatgt cttaggctca tatagcttag catataatgc
     9061 ttctgcttta tactcgtcac ttccccataa taacttactt gatttttttg catattcaat
     9121 cttattttgt ttagatattt ttggattatc taaaaatcga taaaattgta ttggtgaaaa
     9181 tctagactga taatggtgac ttgtgactcc gtctttttcc ataaaccatt gcatagaaat
     9241 taacaagaca actttcttat tatcaatatt aggattcata cttcctaata ttgcagcatc
     9301 ttgtaatgtc tgagtatagg ctctcccaat tgcaaacacc ttatttttac tcctatttgt
     9361 attaaaatag tactttggat gttgttttgt tgaattacta agttctgatg accccaaaat
     9421 cataatatcc ttttctctta aaaaatgatt atttgctgta acccctttat cttttatatc
     9481 acttaaagta tcatccataa ttggaagtag atttttctcc ctcaaaagtt catcagtttt
     9541 agaatcaaga aatttgtcta acccaaataa aaatactacg cctattataa aaggtgtaat
     9601 aaagtatatc aattttctca tattttctct cctctaaaaa tattcaaatt tatgttatta
     9661 ttaatcatat tagttttgat atgtatattc aactacaaaa ttgtaataaa atcgtcaaaa
     9721 ctttttttga tactgttaca aaattgtaaa aaaaaagaaa aaaacgccaa aaataaattt
     9781 ctttttagca ctttttcata cttataattt gacattttaa cacttggtta agtatacacc
     9841 ttaccttaac ttaaaattaa atttatttaa gaatatatat tataaattct tatattttct
     9901 aatataaact atatactatc agctggataa acaatactac tttgtcttct tatctcatcc
     9961 ataatttcca ttactattat tgaattttca tgagaattta tatttgattc tattttattg
    10021 ttatttataa gacttataaa ctctacaagt tcataataca ttccttctat ttctctaggc
    10081 ttttcctctg ctttttcttt tggaatagat atatcttctt ctcttccatc tctgtattta
    10141 ataatagctt tttcaaatag attaagcttt tcaattatta tacttccctc ttctccttgt
    10201 atttcagatg gtatataaga atcagctact ttagaatact gaattatcgc atccatatca
    10261 tcatattgaa atacagctga accttctcca tccactccag tttgaagtat atgagaaact
    10321 gcctttacag tttttggagc accaaataaa ttcaccatag gatgtataca atacactcct
    10381 atatccatta aagctccatt tgacaactct tttttaaagg cattttctat aattccattt
    10441 ttatatttat catatcttga agaatactga caatagcttc caaaaaatct tcttattttc
    10501 cctattttat ataagttttc tttaactgct ttaaagtttg gaacacatgt aattctcata
    10561 gcttccataa gcaatacatt attatctttg gctgttttta tcattgattt gacttctttt
    10621 aaattagatg ctaacgactt ttcacaaagc acatgttttt tgtgttttaa acaagtaatg
    10681 gtttgctttg aatgtaatgc atttggagaa gctatgtata cagcatctat atccttcgat
    10741 ttagccattt gttctaaatc atcaaaaaaa atacttgctc catatagagt tccaaattct
    10801 ctagcttttt ctaaatttct agaatataca gcaaccaatt caaagtcttt aaccctagat
    10861 gcagctctta gaaatatatt tgctatattg cttgtcccaa caataccaaa tcttatttta
    10921 cccatcataa atcactcccc attttctaca ctttatttta attttaataa aaaactttac
    10981 aacttataga tatattataa aataatttta tcaaataaaa aagaaagcta attttaaatg
    11041 atttcaccat tttaacatag ctttctttta actacaatct aaattcaact tatattttta
    11101 aagttatctt tctactatat ctttatttcc cctcaaaaat tttatagtaa gaatttcatc
    11161 cacaaacaat gttactaaaa gtatagttac aacaattggc caataaggta ttataagact
    11221 atataaacta tcaactatat acacttgaaa atatctaact ccaataaatg tcaatatagt
    11281 ccaatataca gaaaacttta gacatactat tcctctacta ttgtacttat aatctgaata
    11341 atcccaatag accttattaa aatatacttt cataagtatt cctgtaatat attctactac
    11401 tgtcggcact actagacata ataaaagcaa tgcaaatata ttattggggt caataaatct
    11461 tgagacctct attataatcg acattgcaat tgcatacatt ggtttaaatg gaccatttaa
    11521 aaatccatct tcttgaaaat gcccttgttt ataataacta tataagtttt caattatcca
    11581 acctataaaa ccatacaaga aaaaattaaa taaaaaatat aatagagaac ctacattatc
    11641 catgaatatt cctcctagct gtttatactt attttttctc atattcatgg atttattcta
    11701 aatcaaagaa attatattta atttatttaa ttaatacttt atattataga ttgtgacatt
    11761 ctttagagtt caaacttttt aggtattctc taacttccag taaattttca agtatcttat
    11821 gtgattgtga tttaatttgc tcatctggct cttttaaatc tggtacattt atacatctta
    11881 taccaccatt gtatgcagct tcaactccca taggcgaatc ctctatgaca atacaatttt
    11941 ggggattaac atttatcttt ttagctgctt ttaagaatat ctcagggttt ggtttagagt
    12001 ttacaacatc atctccacaa actatagcat caaagtaatc ttttaaattt gccttagcta
    12061 atctctttac tgctctttct ctctttgttg atgttgcaac agccattttg tatccatttt
    12121 cctttaaaaa agagattagt tcattgactc ctaatttgat aggagctccc ttcctctcca
    12181 taaattcaat catattttta gttttttcgt catataaatc tattattggt acagaactat
    12241 cgtatatatc tgtaagacct tctattattc ctttacgatt tcttcccata actgatgtat
    12301 aaatttcctt agtcatagta tatccatact tttcaaacgt ctccatccaa aattcaagag
    12361 aaattctctc tgagtcaaac aaaactccat ccatgtcaaa aataattcct tctacttttt
    12421 gcatacaatt cctccaatat tttttgatat aattatatta cactttttat gattaatttt
    12481 acaaccctca atttatccaa ttctaatatt gtaaaattgt aattttcaaa agttacactt
    12541 tggtttactt taatatttga accaagcata taggatatcc acccacctat actgtcaaat
    12601 ccatcatgct ctatatcaag tccaaaatac ttattaatat agttgataga ttcagttcca
    12661 tcaactaagt aagaacctgc tcctattttc ttaatatgag tctcttcttt gtcaaattca
    12721 tcttgaattt ctccaactat ctcttctagt atatcttcta ttgttaccac accagatgta
    12781 ccaccatatt catccacaac tatagcaagt tgaagttttt cctttcttat tctctcaaga
    12841 gctttatcta tagttaaatt ttcagatata tatataatat ctctcaatat ttcttctagt
    12901 tcaattttat tttcatttat cttttgattg tataaatctc ttatatgtac aaaacctaat
    12961 atatcatctt tattttttct gcatactgga tatcttgtaa caccttcctc ttttaaaata
    13021 gctaatatct tttcttcaga atcactttca tatatacata ccatatcagt tctaggaacc
    13081 attatctctc taattttctt ttcttcaaat tcaaaaatat tatcaactaa tctttgctct
    13141 gattcatcta taagtccact tttataactt tcttctacta atagtcttat ttcgtcacct
    13201 gtatggggtt catcaacctc atcagcttgt gaatatccaa atggtttaag aaataaatca
    13261 gtacttaagt tgaaaaaata tattattgga tatgttaact tataaaatcc tactaataaa
    13321 aaagatgtac tcaacattat tttttctgta ttataaagtg ctaaagcttt aggaaccaac
    13381 tctcctatta caacctccat cattgttata attaaaaaag aaatggaaat tgatatagta
    13441 tatataatac tttcactaag attaaataca cttactattg gtaaaattaa ctctttaact
    13501 gtagattctc ccatccaacc taatgcaagt gaacaaagtg ttataccaag ttgacatgca
    13561 gaaagatagg aatttaagtt gtcttttatt attttacatc tttttgcatt attattacct
    13621 tcagttatca tggtatcaat cctagaacta cgaagtttta ccatagcaaa ttcacaagct
    13681 acaaaaaaag catttatcaa aacaagcatt actataagtg ttacttttaa taactcccgc
    13741 ataaagtatt ggttcttata atgtaagaaa tctataagaa ctcaaagttc cccccttata
    13801 ttaataaatt taatttataa tatatattct atcatattta ttaatatatc ctactttatt
    13861 acaaataatt tttataaact aaataacccc ttttaatatt tatgactggc tatttgataa
    13921 atattaaagg ggttatttag ttatcatttt ttatttctaa aaatcttttc tcaaatgatt
    13981 tagttatcta tttatccaat tttttacata atttaatgat taaaaatatt aattttttca
    14041 ctataaagta taaatattat ctgattatta aaataaatat taatactata aataaaaacg
    14101 aagcatgatt tttatttaaa taaatctcaa acttcgtctt tacttactta gatatttatt
    14161 tactttctaa atcctattat ttttaaaacc ttatcttttt aatacttcac cacatttatt
    14221 ctcagtaatc tctccatttt ctagcgcaat ttttccattt acaattacat attcaacacc
    14281 attggctagt aattgtggct ttgaaaaatc aatatcttct gacctaggat aactatagtt
    14341 atccatgtca ataactgcta tatcagctat caacccttct ttaagaatac ctctattttt
    14401 tattttaaat tgcctagctg gtaatgaagt aactcttctt acaatctctt ccaatctaac
    14461 accagacttt ctgtacatat tgaagaaaat tggaaaagct cctcttcttt gtagttcaaa
    14521 ccatgacata tccccaccca cactaaacaa acaatctgaa ccaaccataa caaatggatt
    14581 atcaattatt ttatcatcat gtagtttatc tgggaaatca gctctataca ttcctcctaa
    14641 acaaaagata agttcttcgt ctccatctaa aagtaaatcg agtaataact tgtcctcatc
    14701 tattcctttt tgtgtagcta tttcacgtat tgatttcttt tccatattaa tatctttagt
    14761 gtttagaagt attatactcc ccctatctcc aagtataaat gcttctttta atataagtgt
    14821 tcttccctct tcatttctaa gagcatccat taccccatct atacctaatt caaatagact
    14881 agatgatatt gccataataa attgaattac tctttttttc ttcatacaat gcccagaact
    14941 tcttggtact gtgtcaacca ttatttcaac accattatac ctagccttta atatctcatc
    15001 aaatgcttct atttcaactg gacttaagtg agatacttga accctagcac cacttttctg
    15061 cccaacctct atggcttctt ttactgcatt atatcttcct atgtaatctc taatatgaga
    15121 tgtatatata ccatcatgct ctttaatctg ccttgcaata tcaacaagtt catcaatatc
    15181 agcatactta cttggtatat atgccaatcc agtagatatt ccaaaagcac cacttttcat
    15241 tccatcattt attatatctg taatttcatt tttttccttt tctgtaggag gtctatcctt
    15301 agaacctccc ataacactcc atcttatagt cccatgcccc aacaagaatc ccatattgac
    15361 agaaatacca cttttagata taaggtcaca atattctgta aaattattcc aacatttatt
    15421 tttatctata agatactttt tttcttcttc aagtaataga ccatttttat acatatattc
    15481 atatacattt tcagaagaat atggtgttat agaatgtcca caattaccat ttatagttgt
    15541 tgtaactcct tgttttagaa acttttcaaa tttaccatct agtattgcta ctatttcttc
    15601 atgaacatga gggtcaataa atccaggtgt tataattttt ccataacaat caatctcttt
    15661 atttgtattg ctatttatct tagaatctat ttttgctatt aagttgtctt taattgctat
    15721 atctccataa aaaccaggat tacctgtacc atctattata aatccatttt ttaagattaa
    15781 atcatatatc aaaatattcc ctccttaaaa accaattttt aaataatctt aatgtaattt
    15841 ttatatttga aaatcaaatt attaaatttc atatttgaaa attcactatt atttcattgt
    15901 aattagtcac taagcttatc tttatacata gctcttgcaa ttaatatagt accaaataca
    15961 catataagaa ctactagaga ctgtggtatt ataccttgct ttgataatat aatagtcaat
    16021 acagatatta caattgctac tagacctaat tttttatctt gtaaaaatac ttgtccaaaa
    16081 actgacccaa atagagctgg taaaattaag tttaattttt ctactacttc agctggaatt
    16141 ttagatagta cgcttcctcc taaaattacc cctatagtta gtatacttat attcactaaa
    16201 atagatacag ctattcctat tgtagatatt atcgagcctt cttctgtacc tgattcaact
    16261 tctgctgcct tttgagctgc tattgaacat ggtagcctca tattagatat attaccagaa
    16321 agaaaactca tataagttcc tgggatacct aagattggtg aataagaaac tggttctatt
    16381 atgtaatttg gagcactcat actgacaatt gccattgtac ttgctaataa tgctgatatt
    16441 ggaggcataa taccaaatcc aaagctcaaa attattccag gaactaatgc tgctataata
    16501 cccaaagata gtgttagtct tccatacctt atcataaaag gtaaatattc ttcattgtat
    16561 atagatttta ctttactcat aaaactcccc ccttatatta ctgaagctat tatcattccg
    16621 ctaaacattg atattgtaag cccccactct cttaaccatt ttatattttt cttcttttca
    16681 agcatacaca atattaccat tatagccatt ccacttatac atgcaattgt tccattatca
    16741 aaacgcaaca ccctatcagc atttagatat gcaaatgaac ccaacattgc tcctagagat
    16801 attattggta taaatgattt cttgccacta gatataacat tggttatctt atctagttta
    16861 tgtccaaata gtaatgtgaa tattatccat cctaaagaac ctaatatcat agtccaaact
    16921 gcatttgtaa atgcaagttt tgtcatttca ggagaaccta aagttatacc taatgcatct
    16981 gttccaaatc cggctgacat tgattcaaaa attacagaac ctataaatga taatctcatc
    17041 catgctatag gcccacccat agttactata agcgatacca ttccagcaaa tatagtcatt
    17101 gaaggcccaa tagctgaaat agcactactc ttaacagctg actttatttg tttatctgtt
    17161 attcccattg ttttaccaac tttgcaagat tttatagcaa atataaggga ttgtaccact
    17221 actattgata ctgctactgt actcgctatc cacaaaagtg gatgattagc taattttaaa
    17281 taatccattt ataaaacctc cctataaatc ctcaaatttt attaatattt ctaatatatc
    17341 tatttccaaa cctgttttag tactctaagt atatttccac ctagtacctt ctctatttcc
    17401 tcatcactat atccatgctt tactaaatat ctaacaatgt tttttgaagt ttctgttggg
    17461 ttctcaagac cttttacata ttctactttg ggatgttctt ttgttcctgt tatttttttt
    17521 atatcaaatt tagcagctag tactgagtgt aattttacat ggtcgccata tactgtatct
    17581 ggtccaaaag ctacatggtc tattcctact aaatttttaa tatactcaaa atgttccata
    17641 aatgactcta ttgaatgttc tttattcttc tctgttattg tagtatgagg tgctgcttct
    17701 acacctataa cccctccttt tttagcacat gctattaata cttcatctgg agttagtctc
    17761 tttgtgttcc ataaacttct tgccccaaca tggcttaata ttattgggtc cttgctatat
    17821 tctattacat caagtgtagt ttgaactcca acgtgagagc aatctattgc cattccaatt
    17881 ttattcattc tttcaacagc tttttttccg aaatttgtaa gacctccatc tttctcttct
    17941 tttagtcctg aacctaatgc attggattcg ctgtatgtta tacccattaa ccttatacca
    18001 aatccataca gtatatctat tctatcaagt tcattttcta ttggtgctgc accttctaat
    18061 gttggtatca atgctacttt accttcactt ttagctctgt atatatcttc tactttttca
    18121 cactttataa caaaatcttg atgagctaaa tcacatagtc tcatgcctaa atctattaat
    18181 atatcatccc atttccaccc tgaatttgat gttatcgtac atacaccatc cattaaatta
    18241 tcaaatatag catcataata tcctcttgat aatgcttcat aagcacatct ttgtttacct
    18301 tccctattat attcaaatat atctctttct aggtgttctg gaaataatac tggatgttca
    18361 tgtaatgata taaatatttt ttcatccact atacttttaa ccttagcttc ttgaatatca
    18421 tctaattcaa taaggtattc tttaactctt ttatctcctt tacacatctc aatagatgaa
    18481 atatcttttc ctttttctaa ataactatag gcttcatatc ctttgtaagc tgacttcata
    18541 acaacatcct ccaagttcta caatttttta tatttttcct atgtagcaat tgatgtgcca
    18601 aaatataaat tttaattgta taataaatct tgcaaaaaat aaaaacagta gtaaggttga
    18661 tatttactac tgttttatta aaaataatct tcttagatta tttattcaat atttttgaat
    18721 tatcttaatc ttaaagataa ttttatttat ttctatccat tatagggatt tttttagggt
    18781 gttttgcctt tttagtcccc tatatcactc ccttaatttt tacatatgat ataaagttct
    18841 ttccttcatc agttataaaa ctaccttgtt tttttactcc agataaaagt aaaccatggc
    18901 tctttaactt atctattctt tttctaattt ggtctacact taaaactata tctttttctt
    18961 ttaaaatctc ttgcagttta tttcttccta ataatatatt tttattattc caagtttcta
    19021 tactggtcaa tatacatata ctttcctcaa agaaattaga ttgtttgaaa tctaagataa
    19081 ttgaatcgaa attttcatta actaaagtat tgtttttttc aaatctaaat tgttctggca
    19141 aatgctcgta atctactcta tcttcctcaa ctatagtatc aatataataa attaaatttt
    19201 taagctctct tacatttcct ggccattcat acaatgttaa agcttgcatt gattcctctg
    19261 taaaacattt gttgctattt atttcatcta aaaaatactt acttataaga ggtatatctt
    19321 ccttacgttc tctaagtcta ggtatctcta tatgtaatac attaatccta taatacaaat
    19381 cttctctaaa agaaccttct ttaatttttt tctttaaatc tttatttgtt gcagctataa
    19441 ttctaacatc tattggtata attttacttc cgcccattct cataacttct ttttcctgta
    19501 atactcttag caatctctgc tgtacatcta aagatatatc acctatctca tctaaaaaaa
    19561 ttgttcctgt atgtgctctt tcaaatatac ccatttttcc accttttatt gctcctgtaa
    19621 aacttccttc atcatagcca aaaagttcac tttcaataag tgtatctgac aaagaagata
    19681 gattaactgc aacaaatggc ttgttttttc ttagagattc attatgtatt gcctgggcaa
    19741 atatttcttt tcctgttcca ttttctccaa gtatcaacac agaaaagtct gtagttgcta
    19801 tttttcttgc aatattaatt ttttctttta ttattttact ctcacctaca acactctcaa
    19861 atgtatactt tgatacaaaa ccttttgact gaagtttatt ttgaatcttg tcctcaagca
    19921 cttgaattgc acttatattt ttaatgctta taatactctt tattcgttca ttattttcat
    19981 aaacatttac tttattaatt atcagttttt tgttatttaa atttactact tcatcattaa
    20041 cttcatcttc ttgaaaaaat atttttttga caaccttatc actaaataag tccataaagt
    20101 tattagatat aatttcattt tggtctattc caactagatt agaaaatact ttgttgcaat
    20161 atataaattt tcctaattta tcaacagaag caataccttc atctataatc tccaaaaaac
    20221 tcttcatggt tttattcata gttgtataat acctatatcc acttactgta tcttcattgt
    20281 atttttcctt aataatatgt agtttatcta tagatatatt tagttttgta aagatttcta
    20341 taactgtatt tatatcaatt accttatcac ctatgtcaat tatttgcttt atgttttgtg
    20401 gtatgctgtt tctgcttccc gtaataattc caatctcaca tttagactta tcacaacctg
    20461 gataatatgg tattagatta atatgaatta atcctagttt tctaattatc ttggcagttt
    20521 catctgctga ctctttataa gcatctataa ccataacatc agaatcattt tctatactta
    20581 ttatctgatt tatattttca atatttatag tacgatgtac tattacaata ggtatatttt
    20641 tatcaatatt attatgtata tattccttta tatcattgtc tgaacatact acaagctcat
    20701 agcttgacag ttcaatattt atcctattta catataccaa gttatctata ttacaaaatt
    20761 ctccaaatat attttccaat t
//
LOCUS       DAYAOY010000074        20675 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYAOY010000074 DAYAOY010000000
VERSION     DAYAOY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.074"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            456..1451
                     /locus_tag="ACGI6B_002752"
     CDS             456..1451
                     /locus_tag="ACGI6B_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9752187.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1438..2292
                     /locus_tag="ACGI6B_002753"
     CDS             1438..2292
                     /locus_tag="ACGI6B_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752188.1"
                     /translation="MSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            2292..3083
                     /locus_tag="ACGI6B_002754"
     CDS             2292..3083
                     /locus_tag="ACGI6B_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HFG9752189.1"
                     /translation="MKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMF
                     NSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITK
                     PLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLF
                     YDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMST
                     IPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3368..4747
                     /locus_tag="ACGI6B_002755"
     CDS             3368..4747
                     /locus_tag="ACGI6B_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9752190.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            5195..5908
                     /locus_tag="ACGI6B_002756"
     CDS             5195..5908
                     /locus_tag="ACGI6B_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752191.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(6180..7718)
                     /locus_tag="ACGI6B_002757"
     CDS             complement(6180..7718)
                     /locus_tag="ACGI6B_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9752192.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            8609..8734
                     /locus_tag="ACGI6B_002758"
                     /pseudo
     CDS             8609..8734
                     /locus_tag="ACGI6B_002758"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            8789..9055
                     /locus_tag="ACGI6B_002759"
     CDS             8789..9055
                     /locus_tag="ACGI6B_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFG9752193.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            9104..9688
                     /locus_tag="ACGI6B_002760"
     CDS             9104..9688
                     /locus_tag="ACGI6B_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFG9752194.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(9693..10016)
                     /locus_tag="ACGI6B_002761"
     CDS             complement(9693..10016)
                     /locus_tag="ACGI6B_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9752195.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            10168..10686
                     /locus_tag="ACGI6B_002762"
     CDS             10168..10686
                     /locus_tag="ACGI6B_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9752196.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(10862..11530)
                     /locus_tag="ACGI6B_002763"
     CDS             complement(10862..11530)
                     /locus_tag="ACGI6B_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9752197.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            11645..13060
                     /locus_tag="ACGI6B_002764"
     CDS             11645..13060
                     /locus_tag="ACGI6B_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9752198.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            13259..13741
                     /locus_tag="ACGI6B_002765"
     CDS             13259..13741
                     /locus_tag="ACGI6B_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752199.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            13738..14322
                     /gene="clpP"
                     /locus_tag="ACGI6B_002766"
     CDS             13738..14322
                     /gene="clpP"
                     /locus_tag="ACGI6B_002766"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFG9752200.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            14634..15710
                     /locus_tag="ACGI6B_002767"
     CDS             14634..15710
                     /locus_tag="ACGI6B_002767"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9752201.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            15777..17852
                     /gene="bclA3"
                     /locus_tag="ACGI6B_002768"
     CDS             15777..17852
                     /gene="bclA3"
                     /locus_tag="ACGI6B_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HFG9752202.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            18021..18782
                     /locus_tag="ACGI6B_002769"
     CDS             18021..18782
                     /locus_tag="ACGI6B_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HFG9752203.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            19091..19369
                     /locus_tag="ACGI6B_002770"
     CDS             19091..19369
                     /locus_tag="ACGI6B_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFG9752204.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            19662..20237
                     /locus_tag="ACGI6B_002771"
     CDS             19662..20237
                     /locus_tag="ACGI6B_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HFG9752205.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         7671 a         2483 c         3539 g         6982 t
ORIGIN      
        1 attaatccat gcttgaacat aaataacctt tatgatatga tatcaataat aatattatga
       61 gaggagtgcc ttgatgtcgg agttatataa tggtaaccaa caattaaata gttggagaca
      121 catatattct gtataatcag aaatttcaag tgtattttta atacatttaa tttttgtgtc
      181 ttattctgcc tttatagata actgacatat gacaacaaat agtatttaaa atatatatta
      241 ctttgttaaa ttatggtgta gtaggcttta atctgctaca cttttattaa ttttgtatag
      301 tattattttg ataactattt tgaaatagag agcattaatt tttctagtcc acaggagttt
      361 tctgtttttg tgctgttaaa tataagcaca gaaaaaggca gatatatacc tgtggacttt
      421 ttttatataa attatgaaat atggaggtag ttaaaatgag tataattgaa cttaatgaac
      481 tgactaaagc ttatagaaga tttgagaaaa aggcaggatt tatggggagt gtaaagtctt
      541 tatttaatag gaaatataca gaaaaaacag ctatattgtc atttgattta aagatagaag
      601 aaggagaatt tgttggatta ataggtcaaa atggagctgg taaaactact cttattaaga
      661 tgttaactgg tattattgca ccgtcttctg gagaaatatc tgtaatgggt tattacccta
      721 acaaattaga aaataatttt aaaaaacaat atgctgttgt tatgggccaa aaaagtcaac
      781 tattctttga attgacacca gcagatacgt ttcttttatt taaagaatta tatgatatat
      841 ctaatgaaga atataaaaaa aacttggatt actttatcga attatttgag gtatctgact
      901 atttaaatgt tcaagttagg acgctttctt taggagaaag aatgaaaatg gaactaatag
      961 tagcccttct acataatcca aaaatattat ttttggatga accaactata ggcttagatg
     1021 ctatagccca aaaacaaatt aggtatttcc taaaagaagt aaatgaaaca aaaggaacta
     1081 ctattatact tacttctcat tacatggaag atattaagag tctctgtagt agaagtattg
     1141 ttgtagaaaa tagccgtaaa atatatgatg gttctacaga gaaactattt aatcattttc
     1201 aaaaatataa aaaaataaca gttagttttt ccaatgaaac taacattgta tttccaaaag
     1261 attgtaaagt tgttgaacaa acaaatcata agatatcagt aattgtgcca aaagaaagag
     1321 gaaatgaaac gattaaatgg atttttgata aatatgatgt taaagatgta atgattgaag
     1381 aagaagatat aggtagtatt gtagagagaa tatataagaa aggaagtgat gagtattatg
     1441 agcaactata atgaagcaag ttcaaatgat tcaatgtttt ataaatttaa acagagtatt
     1501 agaaggtatt atgaagtagc caaaattaac tttaaagtgc aacttatctg gagatttgat
     1561 gtggtggtta atatggtgtt tacgattgta aaaattttat ttgcttatat tttatggaaa
     1621 gcaatttttg gtgaaaataa aatagtctct ggttttacat ttaactctat gttgtcttac
     1681 tatataataa gctcattttt agcaggtatt gatatgtcaa agagtaccag tgaagagata
     1741 tgtaatcgtg taaaattggg caccttttca aagtacatga ttattccaat taatgttcaa
     1801 aattatttta ttgctaaaaa ttttggaatg acatgttttt attttgtatt taatatgatt
     1861 tctgcatttg tatggatttt tatatttaaa atagattttg cctttacatc aaatccagtt
     1921 caaatattaa tttcaatttt gatgattttt ctagggttta tttttatgat gaaactccac
     1981 ttctttattg ggattttgtc atttaaattt cttgagatta gtttttttaa aatgataatt
     2041 gaaaatattg ttgaatttat tacaggaaca atgataccac ttgtactctt accagaaact
     2101 atagtaacag ttatgaaggt atttccattt tattatacaa catatttgcc atctatgtta
     2161 ctaataggta gaaacagcaa tgaggcaata caaggattaa ttattctagc attatgggtt
     2221 ttattttttt ctgtagtaaa taaagttatg tataaaagaa tgagaacatt atttgatggg
     2281 gtgggtatat gatgaaaaaa aatttgaaaa taatatttat gttattaaaa atgaaattat
     2341 caaagatgat ggtatttaga ttcgattttt ttggagcatt ttttgtagat agttcattat
     2401 ttatattaca acttctaatg tttaatagta tatattctca tgtagactca atagggggat
     2461 ggcaacaagg agagatgtta atatttatag gaactttctc attaataaat gcgataaata
     2521 tgacgatttt cttttttggt atttatgata ttccacgaaa aatccaagaa ggagaattag
     2581 actactatat tacaaaacca ttagacccac tatttaggtt aacatttgaa aatattaata
     2641 ttggttcatc accattaatt ctagcaagta tacttattgt tttatatgga atatctgagt
     2701 taaactcaga gataacttta gttatgataa taatatatat tttcatggtg ttattgatga
     2761 cattactttt ttatgatgta tgtatcatac ttagaacagt ttcatttttt gttatatcat
     2821 cttctgcgat aatgagatta gaagataatt tattgccaat gaatatgaaa attcctggtg
     2881 taatatatga aggtgttttt aaagtattat tctatttaat cttaccatat ggaattatgt
     2941 cgacaattcc gactcaaatt ttggctggga caataacaat taatggatta atatactcaa
     3001 ttttaattgt atttttgttt acattgttta tgtggagatt ttggaagtta ggtatgagac
     3061 attataaaag tgcaagtagt taataaatta gtaaaaatag acaatgtata actatatata
     3121 aatacgtttc atatgacact aaactatgtg aacaagtatt tgcgctaaag gcactgaaag
     3181 ctataaaatt attaaagtaa ggatttaaaa gtttctttag tagaaatata attgtgtaaa
     3241 aaagcaagat taattaaaac tatatccaaa taattagata taattcttat gcagaaagga
     3301 ggtctgtaaa tgtctaattg tttggaatat attttaaaaa taattaatgt acagaaaaat
     3361 aaacaaaatg agtgatgtat attattcaga aaatcatgaa caagataatc tttatgagca
     3421 agataattta ctggcacaaa gatttaattt atttactcta ttgaagtttg catttcctac
     3481 tatttttatg atgattttta tggggttgta tacaatcgta gatactatat ttgtatcaag
     3541 atttattaat acaaatgctc tatcagcaat taatattgta tgtccaatta ttaatcttat
     3601 agttggaatt ggaactatgc ttgccactgg aggaagtgct attgtagcac gtaagatggg
     3661 aaatggtgat attgagggag caaataaagc tttttcttta atcgtattat caggagttgt
     3721 aattggggta ataattactg gtataggtat tgtatttatg gattatatca ttatatggtt
     3781 aggagcaagt gaagttttaa ttaaatattg tcgttcatat ctttttatta tcttggtttt
     3841 tgcttctgca aatatactac aagtattatt tcaatgttta tttgtaacag ctggaaagcc
     3901 tcaatttgga ttatggctag tgttaggtgc tgggtgtgct aatataattc ttgactatgt
     3961 gttcattgtt ctgttacaaa tgggtattag tggagcagca cttgcaactg gagtaggata
     4021 cctaattcct accgtttcag gtatggtttt cttttcaaaa aataaagggt tacttaaatt
     4081 ttgtatacct agactagata aaagaatatt atttgaagct tgttttaatg gttcatctga
     4141 aatggtgggt caactatcaa cagcagttac tactttttta tttaatgcta tgatgatgaa
     4201 attaattggt gaagatgggg ttgcagctat tacaataatt atttattctc aatttctttt
     4261 aacttcgctt tatataggtt tctcaatggg tgttgcacca ataataagtt ataactatgg
     4321 aagtgaaaat aatacgcagt taaatcatgt ttttaagatt tgtatattat ttatttcagt
     4381 agcatcttta ttggtattta tcttttctat atcatgttca ccaatcattg tagagatttt
     4441 ttctcaaaga ggaagccgag tttatgaaat tgcaaggtct ggattttata tattttcttt
     4501 tagttttatt ttttgtgggt ataatatttt tacttcgtct atgtttacag cattatcaaa
     4561 tggaaaggtg tctgcaatat tatcttttct acgtacattt ggatttataa ctataggttt
     4621 attgatattg ccaaatatat taaatataaa tgggatatgg ttagcagtac caagtgctga
     4681 gattttaact ttatttttaa ccattttttg tatatgtaag tataagaata gatataaata
     4741 tatgtagata ttaaataaat agtttaggta gatattttat gttttaataa gtaattcaat
     4801 tagtgggaga tatatactat tgaaaattaa agttataatt gatataaaga attaataaaa
     4861 ataacatata aaagtttaaa agggagttga acttaaagtt tgattagaaa aagtcaattt
     4921 taagacaact ccttttattt tactattgta ttaatctgta tctgtaagtg tattagtcta
     4981 atattataaa ctttggatga aattaatttt ctataggttt taatgtatca tacactttat
     5041 tttaaagaaa cttatcttaa ttttcatagt gattgaattt gtctattaga gatgttacta
     5101 ttagtaatgt aaaagcaaga gtagtgaata taaaaaacaa gatagtgata ctgcaagatt
     5161 agaatataat aaatttttat ggagagattt caatatggat acatgtttta ataaaaaaat
     5221 attacttgtt gatgatgaaa aggatatagt agatttaatt gaagaagtac tcataaatga
     5281 tggttttaaa aacattatta aagcttataa tggtctagat gctatttcgc tgtgtaagat
     5341 agcatgtcca gatgttgtca ttcttgatat aatgttacca gatatagatg gtatagaagt
     5401 gtgtaaaaaa attcgagagt tttcatactg ttctattctt tttttatcat caaaaaatga
     5461 tgatattgac aaaatactag gtcttagtag tgggggagat gactatataa caaagccatt
     5521 cagtccaaga gaaattgctt ttcgagttaa agcgcaatta cgtagacaac agtatcaaag
     5581 cattgtacca tcagatagtg aagttattaa aataggagat attactattg atatagaagg
     5641 aaaccgtgta tataaagata gaaatgagat tgagttaact ggaagggagt atcatctttt
     5701 aagctacatg gctcaaaatg taaataaaat tataggaaaa gaaagactct atgaacaagt
     5761 atggggagta tatagtagta tttgtgacaa tacaattatg gtgcatatac gacatataag
     5821 agaaaaaatt gaagataacc cctcaaatcc taaaatactt ataacagtta aaggactagg
     5881 atataaactt gtgaatagaa tcgattagaa tagattttaa tattaatgaa gatatctcaa
     5941 gttgaatttt tagttaatga ggcattcttt tatgggtaaa atcaaatata ttttcgttat
     6001 ctcaacaatg aagaagaggt ctatctctat tttgagatag cctcattaag tttatatcct
     6061 ggtaatgtac tttatcttct aataaaattt ttttagttta tttgcagtga aacattaact
     6121 agaacattaa aatttgcatt gaaatactaa ttaaataata ttgtatattg tgtataaact
     6181 tatactaatt gtttttctat attaaatact ttatcaatcc aacctttata aaatttttct
     6241 tcatgggaaa ctaggattat acttcctcta aactgtttca aagcattttg taaagcatcc
     6301 tttgtctctg catcaaagtg attggttggt tcatccagga taagaaaatt acaaggagat
     6361 aacatcatac aacataattt aaccttagat tgttcaccac cacttaaagt agatacactc
     6421 cttgaaacat gctcttcctt cacaccacat ctggctagat gacgtctaat ctcttttgta
     6481 tttaattttg gatatttatc tgctactatt tgaagtgggg ttttatcagg attttcccat
     6541 tttaaatctt gctcataata accaatttta acttgttctg aaaactggaa atctccagat
     6601 atacgaggaa tatctttgac taatgtcttt aacaaggtag atttaccgat tccattaaaa
     6661 ccagttataa ctattttttg acctccatca acagagaaat ttaattttgg aagtaaagaa
     6721 tagtagtatc caacttcaag atttgttata gtaagagcat tttgagccga tatttcaatt
     6781 tctgaaaatt gaatatttgg ttttcctgta aaactaggag gagctattct ttctatttta
     6841 tccaattgtt tttgtcttcc tcttgcaatt tttgaattta ctccagcttt atttttacgt
     6901 ataaatgttt cttctttttc tattttcttt tgttgtgctt gataccttct aatataatct
     6961 tctcttaaat gtgatttttg tttaaggaac tctgaatatt ttccatgata tttcttaatc
     7021 atgccaaatt ctatatcaag aatacctgta gaaatttttt ctaaaaagtc aaaatcgtgg
     7081 gaaactacaa taaatgcccc attaaatgta tttaaataat ttgagagcca ttcaacatgt
     7141 tctttatcta aaaagtttgt tggttcatct aataatagaa catcatggtt agaaagtagt
     7201 aattttgcta aaattacctt tgccctttga ccaccactaa gctctccaac aacacgattc
     7261 attcctattg aatctaatcc aagaccattt gctattttat ttatttcatt atctatagaa
     7321 tagaaattac ttgctattag ttgttcctga taatctgatg ctttgattag atattgctca
     7381 ttttcagata tagcactttt ttgatagagt aaattcattt ccttttctat tttataaagt
     7441 tcttcaaatg cagtgtgtaa atagagtgat attgttgtat ctctattgat ttctgcatat
     7501 tggtctaaat ggcctatatt gatattaggt tgccacttga tttctcctga atctggaaca
     7561 acttctccca ataaaatttt aattaatgta cttttacctg taccattttg gccaactaca
     7621 cctatatgtt cacctttgaa taaatcaaag ttagctttct catacagtga tttatccata
     7681 aaacattgtg ataaattagt tacttttaat aaactcataa ttcaaactcc ttttctttta
     7741 ttaaaaaatt tatggaaata aaaaaagcag aggaatggca ttgtaaaatg ccgacctctg
     7801 cttttggaaa aacaaaaggt catagacaag cattgttgtc tatgaccaga aaatctatat
     7861 tatctatcgg caaataggta attttaaata agcatagcca aaaaactata ctaaacctat
     7921 atttgccgat gaagatagaa agctttaagc tttcagatta acctctacac aacgcttcat
     7981 cgtatacatt tgtgtagagt taagttttct aacaagttta actaattcta tataaatcat
     8041 aattattcac atcctaatct tagtattaat tctagtttat ctgtatttaa attgtacttt
     8101 tgtactttgt ggattatctt atttatttaa aatatgtttt attaattata gcttaagtta
     8161 tgttatcata atacttagtt atagtcaata agaaagttat atttactatt attatattaa
     8221 tttagtaaat ctatcattac aaagttattt gaagactcgc tatcaataaa attttatagt
     8281 ctataagaaa attaagatta tgagttttat aattttgata tagggatact attttcataa
     8341 atattacaac atgtagaagt atatgtctat tattgttgaa ttttcaatga tatattaagg
     8401 ttctaatata ttatacaaaa taaaaacatt aattcaactt aaataatttt aagtattata
     8461 tgtgttttga ttaaagaaaa ctaaaatttt aaggaaaaat aacatatttg tattaaattt
     8521 tattgaaatg attaaattta tttacttaat gtaaattatt agctaaaatt gtattaaata
     8581 attaaggagg tgagtagctc tggatgaaat agatttaaaa ataattaatt ctctaaaaaa
     8641 gaatagtagg gcttcaacct ctgaaataag cagacaaatt aatttatcag taccagcagt
     8701 ctcagagcgt ataaggaaaa ttgagcagtc aaatttaatt caaaagtaca ctattcaaat
     8761 taatcgggaa aaaacaaaat ataagctttt ggcttttata tttatcaata ctgatttatc
     8821 taaaagtatg aaagaattta agaaaactat attggaatat gatactgtat tagaatgtca
     8881 tcatatttca ggagagtatg attacttaat aaaagtatta gcagaagata caagtaatct
     8941 tgaagagttt atatcagtga ctttgaagga aattgggggg attcaaaaag ctaatacaac
     9001 cattgtttta tctacaatta aagaagaatt taatacaata aaagatattt tttaaataat
     9061 ttactagtaa agtgttaatt atattttagg aggaaaatag aatatgaaag ttttagtttt
     9121 tttagcaaaa ggttttgaaa ctatggaatt tagtgtattt gtagatgtta tgggatgggc
     9181 tcgtaacgat tatgggcatg atattgatgt agtgacttgt ggatttaaaa aacaagttat
     9241 gagcacattt aatatacaag tattagttga taagacgata gaagaagtat gtgttgatga
     9301 ttatgatgct ctagctattc ctgggggatt tgaagaattt ggattttatg atgaagcata
     9361 tgattcatct tttttaaatc taataaggga gtttaattca aaagaaaaaa taatcgcttc
     9421 tatttgtgtg gcagcattgc cagttggaaa aagtggtgtt ttaaaaaatc gtaaagctac
     9481 tacatatcat ttgaaaaatg gtaaaagaca aagacagtta agtgagtttg atgtaaatgt
     9541 agttaatgaa ccaattgttg tagataaaaa tattataacc tcatattgtc cagaaacagc
     9601 tccacatgta gcttttaagc ttttagaaat gttgacatct aaagaacaaa tggatgaagt
     9661 taaattagca atgggattta aactgtaatg gtttatagat tttctaagta atcagaaccc
     9721 cagtcacaca tggaatctaa aattggaata atactctttc caaaatcaga tagcgagtat
     9781 tctacttttg gaggaacaac aggataaacc actcttataa ttaaaccatc tgattctaat
     9841 tcacgtaatt gttgagttaa catcttaggc gttgctttag gaattaattt ttttaattca
     9901 ttgaacctca atatagtgtc tttaagatgc caaagtataa gtgtcttata tttaccacca
     9961 atcaatgcaa gtgttgcttc tattgggcaa ctgtaattat ctaaacattt tttcattttt
    10021 gggctccttt atttatttta aagtatcttt ttaggtacta tattacaaaa aagtgcatta
    10081 ttgtaatata gtaccatagt gataaaatta tagcataggt taaataaatc atcaacttag
    10141 cctaataaaa caataggagg taaataaatg aatttcgttg aacttgcaaa aaaaagatat
    10201 tcttgccgta attatcaaga tagaaaggtt gaaaaagaaa aacttgagaa ggttttagat
    10261 gttgcaagaa tagctccaac tggtggtaat cgtcaacctc aaagattgat tgttattcag
    10321 gaaaaagaag gtataaataa actttctaag gctgccaata tatatgatgc tccattagca
    10381 attcttgtat gtggagataa agacaaagtt tggacaagac cttttgatgg gaaacaactt
    10441 acagatattg acacaagtat agttacagac catatgatgt tacaggcaac tgaacttgga
    10501 ttggcaagtg tttgggtgtg ctattttaat ccagatataa ttagagaaga gtttagtttg
    10561 ccagataatc tagaaccaat aaatatatta ttgatgggat atgaatctaa gataccagag
    10621 agcccagaga gacatgaaaa aactcgtgtt cctttaagtg aaattgtttc ttatgaaact
    10681 ctttaattat aaaattaagt tattcatagg ataaatgtaa taaagtgttt attataaaca
    10741 aaattcaaaa taaataatat ctacatataa ttaattttag tgaagtatat ctagtttaac
    10801 tagaatgtat acttcactaa tttatttatt caaaatattt atgaggattt taatatatta
    10861 actaaaatat atcaattgaa ttaactttta aatctggaaa agcacttgaa atatagtgtt
    10921 ttatgtcatt taaatggtct tgaatttgct tttctgatat tttatcaggt ccatatacaa
    10981 aatacataat atttttagta tcttttgtta tagctgttcg attatcaggg ccattaagaa
    11041 tactagataa tataccaata ttatctgata aaaataaatc atcttttgtt aaaatatgtt
    11101 ctttaccacc tataccataa aaatgttcac ttccatttgc taattctatt ttcaaaggta
    11161 attccatttt atcaaggtca tgacctgctg ttagaagaag atttttcaat tctgctaaaa
    11221 acattgactc aattgttaca cctacatctg ggataccttt tgattttaat agaatagatt
    11281 ctaattgaag caatacaggg taagttttct tgaatttttt ataatacttt atataacttg
    11341 aaacaggctc tgttttaatt ttttctttac gattgtattc tgggtgttgt tctataatat
    11401 ttttaattgt agagttttta agaactaaaa aattttcttt attaggtttt gaatgtgtaa
    11461 tatttataac cataactcca aatttaatat tgggataaat ttctttaact ttatgtgata
    11521 tttctaacat aatatagact cctttttatt ttatatttta tgatataatc ttagcaaatt
    11581 aaaagtggat tttgtagttc catttttata gtaattttat agaaccactt ttggaggtat
    11641 tagaatgtgg attacgatta atagaaatag taatatatca ttatcaagac aaatttattg
    11701 tgagataaaa gatatgattc tggatggaag cttaaagttt gagcaaaaac tgccatcatc
    11761 taggacttta tcaaaagaac tatctgtatc tcgtaataca gttttagatg catataatca
    11821 attaattgca gaaggatatt tggaaacaag gaatggatat ggaacaattg tagctaaagg
    11881 aatatcaggg cataaagttg attttaagtt aaatgatact tttagagatg aaaaagattt
    11941 acatactaat aaacaaaaaa attatataga ttttcgttca ggagttccag aattaaaact
    12001 atttccacgc aaagaacttt caaagctata taaacaaata ctgtatgatt tatcagattc
    12061 ggatttcaga tatggaagta cagctggtgt atgggtttta agagtggaaa tatctaagta
    12121 tttatttcgt actagaggaa ttagatgtaa tcccaaaaac attatgattg tttctggttc
    12181 aacacaaggt ttatctttaa tttcacagct tttatacaaa agtaataaaa aggttgctgt
    12241 agaagaccct attcataaag gcttattaaa ggttatttca tctattggat attcagttgt
    12301 tggaattaaa gtagatgata agggaatgaa taccaactta ataaaatcaa taaaggggat
    12361 ttcttttgtt tatactacac cttctcatca atacccatta ggagggattt tacctattca
    12421 aagaagacta gagcttatta aatgtgcaac agagaatgat tactatataa ttgaagatga
    12481 ttatgatagt gaatttagat atgagggtca accaattagt tctctttatg aacttagtcc
    12541 aaataaagta atttacatag gttcttttag taaaatatta tctccagcta ttcgtgttgg
    12601 atttatgtta ttaccagaca atattttaga aagctataaa accttaaaaa tgtactctga
    12661 tgttcataca gaaacaatat tgcaacatgt attagctgaa tttattggaa atggaggttt
    12721 agagaagtac atatttagaa tgaagaagtt gtacaataga aaacgtaagt acctcataaa
    12781 agttttaaac acggaattta aaaatgaatt taaaataaaa ggtgaagcag cagggcttca
    12841 tatagttgct catttctata atgttaaatt tacggaaagt ttaattcata aaatttttga
    12901 agcaggtata aaagtttatc ctatacaaca atatgcaatt gagaatatat tagaatatga
    12961 ctatgaaatt atactaggtt atgctcatct tagttttgat gaaattgaaa aaggtgtaaa
    13021 aatactcagc aaaataatac atatgaatca aggtacatag ataaaacatg tagcaatcta
    13081 catataatag atgtaattaa gtttaaactt taaaagaaat atatattaat atttttgtat
    13141 aaaaatacct gatatttttt tatcgatact ttattgctat acactaaatt aagatagcaa
    13201 tttatattaa gaaaaaactt tttgcatagg ttataatact taaaaggaga ggatagagtt
    13261 gaaaacaaat acagatgtta ttatggagat aattgattat atagaagaaa atcttacaga
    13321 aaatctaaat ttagataaaa tagcttatat tgctaattat tcaaaatacc atttacacag
    13381 aatgtttaca aatgttgtag gatgtactat ccatcaatac atacagagga ggagacttac
    13441 tgaagcagct agacaactta tatttacaga caaatatatt atagacatag ctattacagt
    13501 aggatattct acacatcaat cttttacact agcatttaga aatttatata aagaatctcc
    13561 acaagtatat agaaataaag atgaatttta tcctattcaa cagagatttg ttttaaatct
    13621 aaatcatcaa attcaaagtg aaaaatgtat ttcttatgca aatatgaatt ataaacaaag
    13681 aagagatgaa atcaattctt atctgaactt taatattaat tgtgaggtga aagtagcatg
    13741 agtttagtgc cacatgtaat tgaacaaaca ggtcaagggg aaaggtctta tgatatatat
    13801 tcaaaattat taaaagatag aattatattt ataggagaag aaattaatga tgctattgca
    13861 agtttagtag tagctcaatt actgttttta gaatcagaag acccagattc agatataatt
    13921 atatatataa atagccctgg tggctctgct acatcaggat ttgctatata tgatactatg
    13981 aattatgtca gatgtgatgt atcgactatt tgtattggta tggctgcaag catgagtgct
    14041 tttttacttg caggaggaac acatggaaaa agatgtgctt tacccaattc agagattatg
    14101 attcatcagc caatgggagg agctaaaggt caagcaacag atgtaaaaat agctgtagat
    14161 aatatattga aaataaaaga acgattagat aaaattcttt ctgaaaatac aggtaaatca
    14221 atagaagata ttaggagaga tacagataga gataatttta tgactgcact agaggctaaa
    14281 gagtacgggc tgattgatta tataatgaat aggaatgaat aaagtatcaa agtatcatat
    14341 taaaatgtct gttttacaaa tatatatata atattaaata agagtgtctc aaaatgaact
    14401 ttttagttta tgggacactc ttttttgtat gaaattaaat atggttttat catttcgaca
    14461 attattacca aatttcaagt ttaataaatt ctactataaa aaatacataa aagataaggt
    14521 tctttagtaa ctttctgtcg tcaaaagttt ttataaattt aataacaaaa caagagaaaa
    14581 taccatatat tacttatgtc tgcaatatat agaagacaaa ggagttgtac attatgatta
    14641 caataagttt gtgcatgatt gttaagaatg aggaagaagt tataggcaga tgcttagaat
    14701 gtgtaaagga tatagtagac gaaattataa ttgtagatac cggttctaca gattccacaa
    14761 aagaaatagt ctcaaaatat acagacatgg tgtacgattt cgaatggata gatgatttct
    14821 ctgctgcaag aaatttttca ttttctaaag cttctaaaga ctatactatg tggcttgatg
    14881 cagatgacat aattttagaa gctgatagag aaaaattatt aaagattaaa gaaagcttgg
    14941 atacttccat tgatattgtt atggcaaaat acaatgtatc ctttgatgaa aatggtaacc
    15001 caactctctc atattataga gaacgattgt ttaaaagaag ttctaatcat aaatgggttg
    15061 accctattca tgaagtagta cctctttttg gagaggtatt ttattctgac attgcaatat
    15121 ctcataaaaa gcttcataga caggaccctc taagaaatct tagaattttt gaaaaaatga
    15181 tttcagaagg aaaaacattg gagccaagac accaattcta ttattctaga gagttgtact
    15241 ataatgctag gtataaagaa gcaatagaag gttttactaa gtttttagac tcatcaagag
    15301 gatggattga ggattgtatc agtgcttgca gagatttagc cacatgttat tacttaatta
    15361 atgatgaaaa aagtgcctta tattcattgt ttagaagttt cgaatttgat gagccaagag
    15421 cagaaatttg ttgtgacata ggaaagcata tgtttgatag acaaaaatat aaagaagcca
    15481 ttttttggta taaggtagca ttaacaagag ataagaatga tactgatgga ggctttaagt
    15541 cagatgattg ttatggatat ataccatcta tacaattaag tgtctgttat gacaggttag
    15601 gagaatcaga taaagctatt tattatcatg aaaaaacaaa agaaatcaaa cctaatgata
    15661 gtgctgtttt gcacaatgaa aattatttta gtaaatttaa aaatagttga tgggtcacaa
    15721 tcatactaaa tttgacatat tatatataga gcttaaaagc tcaaatatat caggaggtgc
    15781 ttttaataat gagtagaaat aaatattttg gaccatttga tgataatgat tacaacaatg
    15841 gctatgataa atatgatgat tgtaacaatg gtcgtgatga ttataatagc tgtgattgcc
    15901 atcattgctg tccaccatca tgtgtaggtc caacaggccc aatgggtcca agaggtagaa
    15961 ccggcccaac aggtccaacg ggtccaacag gtccaggtgt aggggcaaca ggcccaacag
    16021 gaccaaccgg tccgactggt ccaacaggaa atacagggaa tacaggagca acaggattaa
    16081 gaggtccaac aggagcaaca ggggcaacag gcccaacagg agcgacagga gctataggat
    16141 ttggagtaac aggtccaaca ggcccaacag gagcgacagg agcaacagga gcagatggag
    16201 taacaggtcc aacaggtcca acgggagcaa caggagcaga tggaataaca ggtccaacag
    16261 gagcaacagg ggcaacagga tttggagtaa caggtccaac aggcccaaca ggagcaacag
    16321 gagtaggagt aacaggagca acaggattaa taggtccaac aggagcaaca ggaacacctg
    16381 gagcgacagg cccaacagga gcagtaggag caacaggaat aggaataaca ggtccaacag
    16441 gtccaacagg agcaacagga gcagacggag caacaggagt aacaggtcca acaggggcaa
    16501 caggggcaac aggagcagat ggagtaacag gtccaacagg ggcaacagga gcaacaggat
    16561 ttggagtgac aggcccaaca ggtccaacag gagcaacagg aataggaata acaggagcaa
    16621 cagggttaat aggtccaacc ggagcaacgg gagcaacagg agcaacaggc ccaacaggag
    16681 taacaggggc aacaggagca gcaggactaa taggaccaac cggggcaaca ggagcaaccg
    16741 gagcagatgg agcaaccgga gcgacagggg caacaggagc aacaggtcca acaggagcag
    16801 atggattagt aggtccaaca ggagcaacag gagcaacagg agcagatgga ttagtaggcc
    16861 caacaggtcc aacaggggca acaggagtag gaataacagg agcaacagga gcaacaggag
    16921 cgacaggtcc aacaggggca gatggattag taggtccaac aggagcgacg ggagcaacag
    16981 gagcagatgg agtagcaggt ccaacaggag caacaggagc aacaggaaat acaggagcag
    17041 atggagcaac aggtccaaca ggggcaacag gtccaacagg agcagacgga ttagtaggtc
    17101 caacaggagc aacaggagca acaggattag caggagcaac aggagcaaca ggcccaatag
    17161 gagcaacagg tccaacagga gcagatggag caacaggggc aacaggagca acaggtccaa
    17221 caggggcaga tggattagta ggtccaacag gagcaaccgg agcaaccgga gcgaccggag
    17281 caacaggtcc aacaggagca acaggagcaa caggagcaac aggtccaaca ggtgctagtg
    17341 caataatacc ttttgcatca ggtataccac tatcacttac aactatagct ggaggattag
    17401 taggtacacc aggttttgtt ggctttggta gttcagctcc aggattaagt atagttggtg
    17461 gagtaataga ccttacaaac gcagcaggaa cattgactaa ctttgcattt tcaatgccaa
    17521 gagctggaac aataacatct atttcagcat acttcagtac aacagcagca ctttcacttg
    17581 ttggctcaac agttacaatt acagcaacac tttaccaatc tactgcacca aataactcat
    17641 ttacagctgt accaggagcg acagttacac tagctccacc acttacagga atattatcag
    17701 ttggttcaat ttctagtgga attgtaacag gattaaatat agcagcaaca gcagaaactc
    17761 gattcttact agtatttact gcaacagctt caggtctttc attagttaat actgtagcag
    17821 gatatgcaag tgcaggaatt gcaataaatt agattagata agaaaaggct tctaatatcg
    17881 tattgatatt aggagctttt ttaagaatga ttgatgaaca tagttttaat attaattagt
    17941 gcgcatatta taaaatacta ttatataata taaaataata acattaaatt ggaaatatag
    18001 aattatctag gggtgatact atgaaaaata agattgaaat atcaatgaac aactttgtaa
    18061 aaaattatca taaatcaaat aatataacta caaagtggca agaacttttg tttgcttatg
    18121 catctgctga agatgatatg tttcagaagc taaaaagtgt tgtaggaaca tctcatgcta
    18181 tgccaaaaga ttttctggaa aatggaaaaa ctataattac atattttata ccttttgatg
    18241 aaagtgtaaa taatagtaat attggagggt ttgaatgttc tgaaatatgg gcaagagcgt
    18301 acatagaaac taatatgttg atttcaaatt taaatgattt cttatgtgaa gaactagaga
    18361 agatgggggc taattcatat gctattcctg ggtcgtataa ttttgacagt gaaaaattgg
    18421 taagtgactg gtcacaaaga catgtagcat tcatagcagg tcttggaact tttggtttaa
    18481 ataatatgct tattacagaa aaagggtgtt gtggaagaat aggaagcata atcacaagtt
    18541 tagaattaga gccaactaaa agaccagaaa atgaatactg cttgtataaa cataataata
    18601 gttgcaaaaa atgtgtcaac aaatgcgtga aagaagcttt aaaagaagat ggatttaata
    18661 ggaataagtg ttatgaaatg tgtatgataa atgaaaaaaa atacacttat ataggtggat
    18721 gtgatatttg tggaaagtgt atagttggag ttccttgttc agtaataaac ccatcaagat
    18781 aatggattta agttgactaa taattttaaa gatagtttat aatgaaaaaa ttttagttat
    18841 caaaataaat ataatataaa aactaaataa atgtgtttaa aaaatggata aataatttgt
    18901 gttgattgac aaccagttat actgttgata gacttatatg tatcgctgtg atatataagg
    18961 agggttgaga ttttatgttt gatttaatct ttatttttgc atagcaaggg ctattgcaac
    19021 tatgaaattt aatatataaa tagccctttg ctattcctaa tgaaaaatta ctatttaata
    19081 ggagagcaaa atgagctata aaaaagcaat acatatatta ccagaagaat tacttgaatt
    19141 aatacaggaa tatgtagatg gtgaatgtat ctatattcct agaaaatcta ataataaaaa
    19201 agaatgggga agtaatacac ctactcgtga ggagttgtca attagaaata tgcagattta
    19261 cgaagactat caagttggat atgatttgca atatttatcg gaaaagtatt atttatcttt
    19321 gaaaagtatc caaagaattg tattacaaga aaaaagaaaa gatatctaag gatgagccag
    19381 tatagtttta taattatact ggctcatttt cttttgataa aagtgtttga ggtatagttt
    19441 cttttaagtg gatgatataa tttaagtaat ttcaataact tatacagtag ctaacaaagc
    19501 tggttgtaaa gtatctaata agaagtagta gctttttata tgtttgaaat taaaatttat
    19561 taaaattagg aggtcttgtt ttgaaaaaaa tacttgatta tattccacct tttcataact
    19621 ttctattaat ttagtaatct tatagaaagg aaattgcatt tatgaaaaaa attacttggg
    19681 aggtggaata tatctcccag ttacctgtaa ttaggtattg taaaaaatgt ggaaaaaaga
    19741 ctgaatatat ttgttcaggt ttatttaggg ttaatgctca acataaatat ttggatatat
    19801 ggttaatata caagtgttca aattgtgatt caacatggaa ctcaactata tattctcgtg
    19861 ttaatccaaa aagcttaaat ccagaaatac tagaacaatt ccatacaaac gattataatc
    19921 ttgttaagca atatgctatg aatatggaac ttttacatag aaatggagca gaagttggta
    19981 ttccagagta taaaatagta ggacaagaaa ttaatattaa tgaccttaca caagttcata
    20041 ttattagtaa atatccatgt cagttaaagg tatcaacatt attacgtgag aaactaggtt
    20101 tatctaggaa ggtatttgac gaaatgttgg attgtgggat tattagaagt aactcaggag
    20161 ttgatttaaa gaaaggtaaa ttaaattatg aaattatgtt agatataggc aaggagaagc
    20221 aaaagtcttc aaattgatta gaataactaa cttaatatat tttcaggaac atcatttaaa
    20281 aaaatagtgg ctttaaaaaa tttaaagcca ctatttttta atatgtttta tattaatatt
    20341 taaaatatag tgtgtattct tcaaaattat agcgataatt aaaatattac tataaaaagg
    20401 aaggtgcata ctttttattt aacaaaaact agatattaaa aacttttcga ggaatatttt
    20461 attaaaatcc ctaaatatat ttaatatata ttaaaaaatt gtattataat gaaaatcaaa
    20521 atagattgca aatccaaaaa gtaatactta atgtccaaaa agctaaaatt aaaatctaaa
    20581 tagctattta ctacagaagt atgttaaaat atgtaaaatg aatagaaatt tatatatttt
    20641 tattaaaaat atataagaaa ggatgtttaa gaatt
//
LOCUS       DAYAOY010000075        19807 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYAOY010000075 DAYAOY010000000
VERSION     DAYAOY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.075"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            402..1805
                     /locus_tag="ACGI6B_002772"
     CDS             402..1805
                     /locus_tag="ACGI6B_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9752206.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2301..3323)
                     /locus_tag="ACGI6B_002773"
     CDS             complement(2301..3323)
                     /locus_tag="ACGI6B_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9752207.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3358..4956)
                     /locus_tag="ACGI6B_002774"
     CDS             complement(3358..4956)
                     /locus_tag="ACGI6B_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFG9752208.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4998..6263)
                     /locus_tag="ACGI6B_002775"
     CDS             complement(4998..6263)
                     /locus_tag="ACGI6B_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9752209.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6405..7832)
                     /locus_tag="ACGI6B_002776"
     CDS             complement(6405..7832)
                     /locus_tag="ACGI6B_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HFG9752210.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7870..8877)
                     /locus_tag="ACGI6B_002777"
     CDS             complement(7870..8877)
                     /locus_tag="ACGI6B_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFG9752211.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9074..10396)
                     /locus_tag="ACGI6B_002778"
     CDS             complement(9074..10396)
                     /locus_tag="ACGI6B_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752212.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10754..12394)
                     /locus_tag="ACGI6B_002779"
     CDS             complement(10754..12394)
                     /locus_tag="ACGI6B_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752213.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12550..13206)
                     /locus_tag="ACGI6B_002780"
     CDS             complement(12550..13206)
                     /locus_tag="ACGI6B_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752214.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13232..13933)
                     /locus_tag="ACGI6B_002781"
     CDS             complement(13232..13933)
                     /locus_tag="ACGI6B_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFG9752215.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            14741..15220
                     /locus_tag="ACGI6B_002782"
     CDS             14741..15220
                     /locus_tag="ACGI6B_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9752216.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            15217..15996
                     /locus_tag="ACGI6B_002783"
     CDS             15217..15996
                     /locus_tag="ACGI6B_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752217.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(16246..16746)
                     /locus_tag="ACGI6B_002784"
     CDS             complement(16246..16746)
                     /locus_tag="ACGI6B_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9752218.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(17358..18065)
                     /locus_tag="ACGI6B_002785"
     CDS             complement(17358..18065)
                     /locus_tag="ACGI6B_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFG9752219.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(18868..19737)
                     /locus_tag="ACGI6B_002786"
     CDS             complement(18868..19737)
                     /locus_tag="ACGI6B_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFG9752220.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT         6908 a         3495 c         2245 g         7159 t
ORIGIN      
        1 aacaaatcct ataattatat tctaatatac tacctactac tttgcgctac aacaatttct
       61 ctatttatat gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa
      121 tttgtaatat tttattcaat acttaataaa tacttaatca atacatgctt tataatactt
      181 tactcaatat ataatcaata cataatttat aatttgtaat actttattca atacataagt
      241 agaaatctct atatacataa attcattttt tcatacatct tacaaattta tttttattta
      301 gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat
      361 ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact
      421 tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata
      481 atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac gttgctggat
      541 tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat
      601 atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag
      661 gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta
      721 ctggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat
      781 ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag
      841 gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag
      901 ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct accaatagac
      961 ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac attcatatag
     1021 gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta
     1081 tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc
     1141 atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta
     1201 ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa
     1261 actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc
     1321 ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac
     1381 aagcattagg ttatgttctt caattacctg atgaagatag atatatttat actaaggatg
     1441 agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta
     1501 atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa
     1561 tggtttgtga atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga
     1621 ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag
     1681 agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat
     1741 ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa
     1801 actaatatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg
     1861 ttaaagttat aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt
     1921 cattttgaga cacttacata ttttaattac atattttaat tttgtctatt tacagaatat
     1981 tatatctaca taaaactaaa ctgctgtaaa cttaactacc tccagtaatg tttgaagtgg
     2041 tataaaagct agtagcctag tttctacact tataatttat attttacata actaaattta
     2101 gtagattttt atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat
     2161 ctacaactta attaggtatc tatcacttga ttaaatatct atcacttaat taggtatcta
     2221 tcacttgatt aaatatctgc catttaatta aatatctacc acttaattaa atatctacca
     2281 cttaattaaa tatctaccac ttaaagtgta gaacctcttt caatcaattc aggttttaac
     2341 atttcaacga ctgactctgg attttcttca tttaataagg acttaagtat gttaattgtt
     2401 ttttcaatca acaaatctag tggctgtcta atcgtcgtta aattataact cttccatgaa
     2461 gccatttgaa tatcatcata tcctatcact gataaatcct ctggcaccct caacttgtac
     2521 tcaaaccttg cagcatccat tacaccaata gctatcaagt cacttgtgca aaaaattgct
     2581 gttggaggat ttgataatgc taacattctc ttacctgctt catatccaga atcataatca
     2641 tagtttgcag attcttcttt aaggttatga atcccacttt gtcttaactt agagtaaaat
     2701 ccaattttct tttccatttc ttcacttgtt tctctcgtaa attgaatata tcctatattc
     2761 ttatgacctt tcttaagcag atattcagca gccattcccc caccttcaat tgggtctaca
     2821 tagactgtac ttatttttat accaggtata aaacgattaa aaagaatcac aggaatattg
     2881 ttttcttcac aagtctcagc catcacctta ttcatggccg aagctgtaat aatcacagct
     2941 tctacttgaa actgaattac ttttgaaatt atactatcta tactttgttg tctaggaact
     3001 ttaaaaacga gacactgttt acctatctct tgaatttttt ctacaaataa attgataatt
     3061 ctattgtaaa atggacctag actatcacca actatcaatc ctataacatt agtttttcca
     3121 ctaatcattc cccgtgctac caaattagga acatatccta tttcatttgc tacacgaaaa
     3181 attctttctc ttacttcagg ttttacgccc tttccatttg cactaccaaa tgctcttgaa
     3241 acagatgatt gagatactcc tgccaatctt gcaacatctt ttgaggtagc cgatttatta
     3301 taatgaactt tcttttgatt catttagtat ttctccttaa atatctagta tttcttctta
     3361 aaattgtatc aaaaataata taatatattg gataataaaa aaagctaata caattaattc
     3421 ttgtttacct aaagaaaatg gcactataga tactccatct actttatcca ttccttgaga
     3481 aataaggtga tttctcatac caaatgcagt gtatttttta atatcatctg cattatgatt
     3541 tttctttaaa atgacactaa aaatatatcc aaatatcaat gttattgtag cacctattgc
     3601 tggattccac aaccaactca cattatattt attagaaata aaaaatccta aaactaaccc
     3661 tacaataaaa ccaatagata ctcctttatc attagcttta tatgtaaaca ttgctagtac
     3721 aaatgcacct gtagctggtc cagcaaagta tgagatatag ttaccaacaa ggtcaagaat
     3781 tgatttgatg cttcctccaa atcccataat aataaaacct gtcataacaa ctcctataac
     3841 tacagttata atcattgtaa cttttaatga tgattctttg ttatcttttt tgaaataaaa
     3901 ctcataaata tccttcgtaa aaacagttgt catactgttt aatatagagt cgatactaga
     3961 cattgctgct gcaaacacac ctgcaataat aattccaatg gctcctactg gaagttcatt
     4021 taaaataaaa gtaatcatta tttcatttga agtttcaaat gtccttcccc cataaaaaac
     4081 aaataaaatt actcctacca atagcataaa gtagtaaact aaattcataa caaatgcact
     4141 aactacaaaa gaattttttg cacattttaa agatttagat gttaaaactc tttgtacttg
     4201 agattggtca aaacaaaagt atcttatcca cattacagtt ccaccaatta aagtagccca
     4261 aaaagtattt gtcactgaaa tatccctact gaaatcaaat gtatttaatt ttccactctg
     4321 ttgtgcaatt tgtaatgtat caaagaaact tagattcatt ccttttaagg ctacaataat
     4381 tactataaat acagcactta ttactactat catttgaata ctatcagtcc atataactgc
     4441 cttaatccca cctaatagag tgtatataat tgatacaagt acaactattg ggactaaagc
     4501 ataaagtgac cagccagtaa tcatttgaat aataagcact ggaatatata ccatagaact
     4561 agcttgaatc aatgaattaa taaaaaattg aataatagtt aatcctcttg aataatttcc
     4621 taaacgaagt cccatatatt catatataga ggttattttt aatttataga taactggggt
     4681 ggtaatgctc agtgcaaaaa atattgctaa aggtactgta atattaacca ttactggata
     4741 cattccatct acatatgccc atccaggacc acctataaag ccatttgcac taatcatagt
     4801 agctgcaact gataaagcaa ttggaatcca tggcattgac ctacctgcca agaaataatc
     4861 ttcttgattt ttattatctt ttcctgaata ataaccaata actaacatta atagcaaata
     4921 tcctattata atacatacat caattgatga aatcataagc cacccctttc taagtaacaa
     4981 aataaattcc tagtatttta ttttttatta tttaacgtct ctggtaagaa taaccataca
     5041 atagcacctg ctacaaagac tgcgattgat atgtaaaaga tagtcatcat acctaaagta
     5101 ccagaagctg taccagctat agcaggtatt gcaaacgtag atataatcct tcctccatta
     5161 aatgacatac cagatgacaa cgaacgatat ttacttggga acaactctgt gtagtatgct
     5221 ccccatgctc cagaatatcc aacagcaaca cctagtaata ctgatatcat atagaatgtt
     5281 gttgtattat ctccacctaa ttttgtatat ataaaaacaa atactgctgc taatgccata
     5341 cctataaaag gtatttttct tccaaattta tctgcaattg aaccccagaa taaatatcct
     5401 actaattgac caattccttg taaactatat atagactcag ccactgctgc tgacattcct
     5461 attcctgtca ctaggtatgt agttgcatta tttgaaaatg ctgaatatgc tgtaaaattt
     5521 gcacctgata aaagaatcgt tcccaatcca atccataagt attttccctt atacatactt
     5581 atatagctaa tcttttcacc agtttctttt ccctttttaa ctaattcctt tgtatactcc
     5641 aaatcatctg gcacaaagaa gattgctaag atagctccaa ctataggtgg tataatcgca
     5701 gtatacatca atatttgcca atttgcatca cctaaaacgc catatacttt cccaccaaaa
     5761 atcattgcta atgaaaataa agcagttaat atagcagcta acttaccacg atatttactt
     5821 ggaaacatat ctgcaactaa aggcatagct gttccaaata cgcctcccaa cactactcct
     5881 gtacacaaac gtgctatttt ccataacata atatttgtag gagataacat tggtataaat
     5941 gttgtcaatc caatcagtaa agcaacgcta attaaagtcc tcttcttccc aatttttgta
     6001 gaaatcaccc caaaaataaa tgtacctgga attgtaccaa aagacataac tgcaaatatt
     6061 tcttctgttt gttttaatga aaaaccaaat cccccatctg ctaaagcata aatcattgtt
     6121 ggtttaataa aagtacccac cataaaattg tagcaataaa aaaagtatat taaagcaatt
     6181 gaaaaaaatg caattcctct ttttaggttt gaatagtctt ttcctaatat gatatcattt
     6241 tctatttcaa ccgttgtttt catattttta taattccccc ttttaaattt agtcataatt
     6301 ttgctaaaag tttaaacatc tatttttcat atacattccc cacttaaatt ttataaagca
     6361 atacttccct atatatttca aaactattta tactctaaac agctttattc ttctactccc
     6421 atataaattt gttttaatac atccatacca tcaaaagtta tccactcctc ctttacttta
     6481 ccttgtagta tatgataatg attgattccc attacttcta tatgctttcc agaaggctgt
     6541 ccaaaaaatc ctataccttc atttattcca cgtaatctcc atcttacaga tacatcataa
     6601 ccttcatttt tttctctttg attacaagtt actctttcca cttcataact tccatttgga
     6661 attgatgcaa ataaacttac aatcattcct tgaatctcat catagccatt taaatcttta
     6721 tcacatataa aatgtactac tgcattatca tgatagtatt ttttaacttc atttatatat
     6781 ttataattat aaatacggct tgtcatttca agcatcattt cgccaacaga atcatctttt
     6841 gcttgatact tagttggcat aaattgacca tccatagatt cacagatacc ataagttgat
     6901 tgtaaactaa gcactttaga ttcacttgcc tttgccatac tctttgctac ttcctgaggg
     6961 tttaatccta attgagtaac aatccataaa ttatctctaa ccaaccattc ttcatggatt
     7021 ctattgttag ttgtggcaca atcaattaca gttctaaagt ttatttttct acctgttgct
     7081 ggtccaaaat tactatcacc taagttagtt gctgtagaca aaacacggtg agaagataaa
     7141 aaaccatcag caccaaagtt agaccatatt acattttgac caattaatct tctatcagga
     7201 aacgcatgta atgtttgaag agtattggaa ataacatctt taataccaac taaatttgaa
     7261 gagccacaat gcattgtaac attattgtga tatgtatcat aaataacacc tatacctttt
     7321 tcttcccaga tattatgtgt aatcttcata atataatcta caaaatctct atacttagaa
     7381 tcaaatccag gtatttcctg ttcccttttt gtctgttttg aaaaatcatt gtaatccatt
     7441 actccaacag gcgttacttc taatgaatct ccatagtaaa tttttgtaga tgacatatta
     7501 tcttccccct taaatataac ttttttattt ttttcagtaa gagtatttgt atttaattta
     7561 tctgtatcca ctgtatctat gttaacttta tttatgtcgg atttatttgt gtttgtttta
     7621 tctatattcc ctttatctat atttccttta tctatatttc ttttatatgt gttcgtttta
     7681 ttctcatttg tttgattttc atttatatta ctaaaattag tttgattcat tttagctttg
     7741 ttttctttaa ccgtacatga ttcaagccca cttgtagaaa cttctttttc ttcctcttta
     7801 cttgttgcat tatttttgtt ggtagatgcc actttatatc cccctttgtt ttagaatacg
     7861 tatgcataac taagcattaa aaatactttt agttctctca agaatatcaa gcccttgttg
     7921 tttcaaccaa taaggaatat caattaacac ccagttttct tgaagtttat cgcccttacg
     7981 ataataaaca tcaacaactt gcatatctgc tttaacctct ccacctggta atcctaaaaa
     8041 acctccacat ggtacattag tcagattagg ccatccaaag aaacaagcaa aattaccttc
     8101 tgcaaatcta catacatgcc cattaaaagt ctttcctttt aaattatttc taaatggtag
     8161 ctggtgttgc tcttgatatc taggtatagt ataagaagca ccaattccac ctggaccata
     8221 ccaaatcata tctttagccc aagatttttc aagtatttct ggtgggcatc ccatagcacc
     8281 actttcattt agttcagata aatcatctac cattttattt actaatgcta aagtttttac
     8341 tccttcaact tcatctgcat cttcaaaaag taatccatta tggtcaattg gccctggata
     8401 tacaaaatat gaacctgttt gtggtggcaa tggattaact cctgcttgaa tcataaaccc
     8461 aattatatct acaaataatc cagttttagc aattttacca tcttgaacac agttaaattc
     8521 tgcatatctt aaactaatca ttttacgagt atgtttaatg ccaagccaat cgttatcaaa
     8581 aagtcccata aaatgcccca tactcattac ccattcacta ttgtcaatct catttgttcc
     8641 tgcaatgaaa atatcttgtc gtctttgcat attactcaat gattttttta aaggcttcca
     8701 aaatacatct gctacatttt cagtgccttc ttgttcacga aatggataca cacctttcca
     8761 gttataatct gatttcatat gttgctttaa gacattttca acctcatccc cagtagcatt
     8821 ttctaatgct tcaaaataat ttcttacaac tttttttgaa tcctgatatt ttcccataat
     8881 tatctcctcc cagatttatt taagttataa attaactttc tcatttcatg catacgattt
     8941 cctttttaat gattttttag ctgttttaag tttattttat gaatacatat gcatttttct
     9001 aaatattaac atatatctaa aaaattgtaa agaaaatgtt atcataaaaa tttaattttt
     9061 gacttatatc tatttaaaac aacagaaaag gtttaaaacg taatattcta ctgggtctac
     9121 catacttgct cataagctgt tcactcacta cctcacaata accagaatca cacaatctaa
     9181 gtataatcct gtccatagtt ctaatactta caccacatat agtagctaat tcctttgatg
     9241 taaattcagt ttttccacta tttattattt tattgaatac tttatataca gtattagcac
     9301 ttagccctgt ctctgtagca atttgataaa aacgttcatc aaaactattt ttctctttga
     9361 cagtaggatt tggctttagt ggtcccataa tttctccatt ttcaaaaact acatatgctg
     9421 catcattttt ttgactttga gccatcttaa tactctcata agcgttaaat ttagcttcat
     9481 ttgctgtttt tccataacca attcctatat aagtgtttat aactgaaact tcacgaatca
     9541 tatccaacaa ataaatatct ctgtatttct ttgtttctaa ctcaagtatt tttttagttg
     9601 taaaaatcat aaattcactt ctactctgct ctaccaaaac accatctata cggctattga
     9661 aatggtatag tttatcaaga atcttaatcc tttgtgaaag ataagcgtac tcatcttcct
     9721 tcattagtga atattctgaa ggcatgttca gtttaatagc aagaacaata atctgattct
     9781 cattgttttt ctttgcaata taacgcatct gaatgttttt tatactttgc accattacgt
     9841 cctcagaagg caaggctaaa actactggaa tattatcttt tttcattata tgataggcct
     9901 cttcaagacc tgtaatacaa caacatacat cattattttg ataattatat ttatgaaatt
     9961 caagcacaaa ctttatatag tcctcttcta aatacttttg ctctgcaaaa aacatatgtt
    10021 catccttaaa ttcaactcca atttctttat acaatctctt catcatagcc ttatcataag
    10081 tatctatact aagtctacta atatcgaatt taagcaaata tgtaatttca agcaacactc
    10141 tgtacaatgt tgtttcatgt ctaggaatat aatcagacaa tacaaaatct ttttgtcttt
    10201 tttcaaaaaa tttaaatggc gacttacctg taaaaagaac tgcatccact ctacctctat
    10261 atttttccaa taaatcaggt acttcaatat atctatcata tctaagaaaa tctgcttcaa
    10321 tattaggaaa attcttttta cttactgcta gtattttatc gattaccata cctggtccta
    10381 cataaagtat tttcatttcg tctctccttt cctaatgata ctattatctc ttaaaacatt
    10441 ttctaaacct accatgacta aagccacagg actcctgatt catagaattt tgaataatca
    10501 attataccta atataaaaag ttactttaag ttttttaatt acgactatct gttgaaacat
    10561 ttttgtattt aaatttttgt atttaaatct tattcattaa tattaattac attttacaat
    10621 taatatattg ttttttctac ttaaagttaa attctttaga aaacacaagt agagtaatct
    10681 ttattaattt aacaaaaata gccttcctta atattcaaag aaagactatt tttataaaag
    10741 ttcttaatgt attctatttt gctatgctaa gaggattttt aattacacgt ccagcacata
    10801 catcagtttg ctttcctcct gccaatgcaa ttttaccatt caccaacaca tattccactc
    10861 ccactgtata ctgtttagga ttactataag tcgctttatc ttctattgta tctgggtcaa
    10921 ataccacaat gtcagcaaaa taattttctt taagtagacc cctttcttta taaatgccta
    10981 agcgttttgc tgaaagataa gttatctttt taactgcttc ctcaaaagtc aacatacctt
    11041 tttctctaac atagtaagag aaaattcgtc caaaagtacc aaatgcacga ggatgaggct
    11101 ttccaagaga aagtaaacct tctgtagaaa ggtccatact atcagaacct atacatgact
    11161 ttggatggca aacaacctta tctgtatcct cttgaccttg tgtatgaaca actacttgaa
    11221 ttttactttt ttcttcaatc aaaagcctaa agaacgtatc aaatggattt tcaccattga
    11281 ttttggctac ctccattaaa gttttaccct ctacatattt atttgcttca gtcattacag
    11341 aggctagtac aacaccgttc cacccacctg ctaattggta gaaacattgc catccaggaa
    11401 tcccttcctc caactctttt ataattctgt ctctattttc ttgtacttct aatcgtttta
    11461 ccatattttc tacaccgcct tcaaaagccc aaggaggtaa aagtgcaccc aaagatgtca
    11521 ttccagctgt atatgggtaa caatcaaaag ttacttccaa accatctgcc tcagcttcat
    11581 ctagcttttt aaatacctca tcaatagttc caaaattctt atgacccata acttttaaat
    11641 gagaaatatg tagaggaact cctgcagtct tagctatata aattgcttca tcaagagctt
    11701 ccatttcttc atcaccttca tctctaatat gagatatata gtatccattg tatggtatca
    11761 attccttaca taattcagcc atttcttctt tctttgtgta tgctcctggt aaataaacta
    11821 atcctgatga aagagctaca catccagctt ccatagcttt tctaacttcc aacttcatat
    11881 ttttaagttg ctctaaagta gctggttcct ttgaaaatcc catttcattg atacgtacag
    11941 gtccttgtgc tacataagct acaacattga ctgctggaga attttcagtt aagtaatcaa
    12001 agtattcttg cattgtattc caatgaagct ctaattttgt tggaattgta ccaattatcc
    12061 tagttccaag ataatcaatt aactcttgct tcttctcttc tcttatcgga gcaactccta
    12121 taccacagtt tgcaataaca tctgttgtta cacctgtatg gatacggctt cttgtgtatt
    12181 tatcatacac aagagataaa tcagaatgag tatggaaatc tataaaacca ggtgatacaa
    12241 attttccagt tgcgtctata gtctctttag cctctttttg acttaaatct ccaatagcca
    12301 tgattttgtc tcctacaatc cctacatcac ttttatatct tgaatttcct gtaccatcaa
    12361 cgattattcc attgacaatt ttaacatcaa acatgctttt tctcctttca agacttattt
    12421 atattcatat ttcttaataa acaatcctat tcttttttct caacaaataa ttctagtctt
    12481 tctccttaat aaacaatcct attctttttc tcaacaaata attctagtct ttctccttaa
    12541 taagcaatcc tattcttttg ttttattgtt atcaattaca aagaagaccc ttgtgataag
    12601 tatcccacct aacacaataa acaatgttaa taatccacct ggtataccaa ttttttcacc
    12661 aaagtacata attgctaacg ctacagcaat cgtacatcca ccagctcgaa tatccttacg
    12721 tgcaatatca acataaattg ctccaaataa tgctggaagt atataattaa atgaattagt
    12781 aacaaattca ggtaacaaag gaattacttt atagcctaca atggtaaaaa tggtaatcat
    12841 agaagctgat acaaagattg aacaacatgt accaattgtt ccaacaactt caccctcatg
    12901 agttcctgct tctacacctg caattttctg tgccattgat gctgcaggca tacgtatatc
    12961 tccaacagaa cctgcaagcc aacctatata agaacctgga gctcctagtg taggataata
    13021 agctataggt tgaacaatcc aactaacccc ataagtcgca gcgactaacc cccaaatttg
    13081 aaatataaca gaaagcattg gcatctgacc aaagcgaata cctacataga tagcgggaat
    13141 aaagtttgca ataattgcaa gacctataga aattctccct atttttatca tctttttatc
    13201 atacataatt atcacctctt atccgtttta atcacgataa tataatctgt gtaccaaaca
    13261 taccaattaa catggcaata cctaaggata attcttgtaa tcgttgattc tttttaaata
    13321 aagttgtcaa tactagcata actatagctg aaataattgc tggtgtaagt tttggtattt
    13381 caaatgtttt tatttgattt gaaagaaggt aagcaaataa tccaactgta gcacctgcaa
    13441 gtagtttttt aacccaagta ggattatact tcatgttcat cttttcaaca attccacgca
    13501 ttctatgacc caaaataaat acaacaaata gccaacctaa gttatttaaa gccatccccc
    13561 atagtgcata cgaaaaagct tctgctccaa atgcagcact acctacttct accccagata
    13621 aattggatgt aagagatatt actccaagct ctgttctagc tgctccgaca tcacagagcc
    13681 tcatccaagt cgtaggagcg ccaatcacag ctaccagtga taataatgta ataacaggag
    13741 atagagaagg tccaatggct gtaacactag ctgagcgaat tgctgcttta attcctttct
    13801 tttcaatacc aagatttgaa gcctctttta gtgcctcttt cataaatacg attgcttgaa
    13861 caactgaaat aattactaaa aaactagaaa caatccaaag acctgggcta ttaatcattt
    13921 cttgaaactt catactttat tcctccttaa aataaatttt tcagcatttt attgtgagct
    13981 ttctttctgt aatcttgtta tgtttttata ttgttttaat ttattttatc taagatatag
    14041 tatctttaat aggagattat ctgtcttaaa gtctcactaa aaacattaat acttaaatat
    14101 tttattcata attagtaaat atcagaaaat tcacatatta tttttttaac ctgtaattaa
    14161 cttataaggt cttttattat tttgacattt caatataaaa attttaatca aaaaacttta
    14221 ttattttaaa aaaaattatt atttttataa tttatatttt ttaagtttat ttatgttcat
    14281 ctctatctaa aataatgaca tttctaactc ttaattctca tttaagtttc attgtttttt
    14341 cttaaaagcc tacttttagt caagatactg ttatctttac tcattattta aagaacttat
    14401 tttaaatctg tacatgaagt aaataatcca aaagcattgt tattctaact actcaacatt
    14461 aaatacttac tattttctaa ttactcaata taaagactta ctataaaact aaatacataa
    14521 atggcttcta aagtttaata tatctacatg gctttatata tgacaggaaa aatactagaa
    14581 aaaatatatt atagaatact tgtatataat aggcaaaact ttcaaactta aatttatcaa
    14641 ataatcataa aaaaagtgaa ccatctatag taaaatttta tctaataagt gaaaggcaaa
    14701 aaagtagcgc tacgatttgc atagaaggga aggaacttag ttggataaaa ctacatttac
    14761 aaacaatata cttgaatcag aacagacttt gtatcgagta tctaagtcta ttctaggaaa
    14821 tgaccaagac tgtgaagatg ccgtcaacaa tgctatttta aaagcatatg aaaagttgga
    14881 ttctcttaag gaagaacaat actttaaaac atggctgatt agaatagtca taaatgaatg
    14941 taattcatta agacgcaaac gattaaaatc tctatcattt gaagatgtct tcaaaaacaa
    15001 aaaaattgat gaaaaagatg actatagcga tttgtatact gctattcaaa gtctacctaa
    15061 aaaaattaaa attccaatag tcctttacta tattgaaggt tattcagtag atgaagttaa
    15121 ggaaatatta gatatacctc aaggaactgt aaaaagtaga ttatcaagag gaagaagatt
    15181 attaaaaact aaattagaaa acacagaggt aatcatatga ataaaaatat attaaataat
    15241 tcgtttccag aagttccaga aagttttcat aatagtttaa gcaaaacact aaatagttta
    15301 ccagaaagag aggaaaatta taaaatgatt aataataaaa cacacaaatt accatttaga
    15361 aaaggcttag ttgcaacttt agccattact ttgatattga gcacaacagc ccttgcagta
    15421 ggacatatat atagtttagt tggaaaatca agtactaagg ctacctatac atctatccca
    15481 actacagaac aagtaaaaaa agactttaac tttaccccta atatagtttc tgaatttagt
    15541 aatggatata aatttaaagg agcatatact gtaaataaaa aagcatttga caaagaaaat
    15601 aatgttttag gaaaatcaaa agcacttagt tttgaatata aaaatggcaa tgatgaaata
    15661 tctcttgaca cagcaaataa tgtacttggg aagagttctg acaatctaaa agttgtagat
    15721 acttacaaag atattaatat ttcttatgaa gcattcgcac aaaagtttat accagaaggt
    15781 tataaaatga cagaacagga taaaattgat gaaaaatcag gcaaatacac atttagtgtg
    15841 gggccaaata atgaatcaga caaagttgaa gttaatgaat ttaagttttt gacatggaaa
    15901 caagatggag tttactattc ttttacagtt caagactcaa atttatctat gaatgactta
    15961 gttaaaatgg ctcatgaagt aatagatgca aaataaacat aagtaataat aagttgttta
    16021 tgagtttatt atttatgact ttaatttttt tattagtaaa catggttgta tcttccatct
    16081 ttatattttc tttaaattta atatataaaa ttttagatat ttagctacct tcaaaagaag
    16141 cattattaaa aagcccttaa aaatatttct tttatatgga attttaaggg ctttaacttt
    16201 attttataca agtgtatatg gaattttaaa gactttaact ttattttata taagtgtaag
    16261 ctccatttga attacttctt cttttacagg tctatcattc catgtaaaac cactagatat
    16321 tgtatttacc tctacaaacc ctaaagattt ataaacatga atagctctac tattactttt
    16381 aaatacatca agctcaattt tttcataccc atattctttt gataaatcaa taatttgttt
    16441 taaaagacta cttccgatac ctttttccct atactcttta agaataccca tgcctaaata
    16501 accaactttt tcagttttag gtaaaacgtc acaccaacca atacagttat ctgattctaa
    16561 atcaattata aacagttgtg gtaagttttt atttataata ttttttataa attcaactgt
    16621 ttcttcaaaa ggaaaggctt catccatagc tagatatttc ctctcttttg ctattttatc
    16681 aaaagtttgc caatacgaat aaatatactt ttcttctgcc tttataaatt ttatgccttc
    16741 tatcaactta acatttcccc tttttaattt ttgattgtag ttaacttatt ttatatgatt
    16801 ctagtttata atgctatata aattactata tattagctta aaatatcttt gtatatttct
    16861 ataaaattaa aaccaatata tctatctctt gcatctctat taaaataatt tttaatagta
    16921 taataaatat agttctttaa tcttttgggt atatatactt aatcattttg aaatttgatt
    16981 taaagtataa taaaatatat aaaaaaatat ataaaatggt actataaaat ttttaatctt
    17041 catcaatacc ttttttgctt tcattgttaa aactattttt tctattagtt tatttcttat
    17101 aaaacaaaca tttagtatta aaatatatat cacacaatag tatttgcaat ttaactttaa
    17161 aaaaataaaa atacccaact gacctaagca actcgattgg gtataaataa tctaaaaaat
    17221 catttttgta taaactcttt atttaactta catttttatt taccttctac tttaattcaa
    17281 cttatatttc tatgcttctt atactctcat tcaacttata tttctactta tcttatactt
    17341 ttataaattc atcacaatta tttagttctc gctatcaaag aaattgtatt aaaatacttt
    17401 ccattctcta cttccatcat ttttttgtca tttatagcat atgaattatc acaatttatc
    17461 caatccttcc aagcttcatc atgacaattt aaagcttcac atttctctac aatcacagac
    17521 tccgattcag accaaagttt attccaccat gtaattgaat ggaagttcat attatcttgc
    17581 caaaatggaa tgagttcatc tggtatacaa tccacgaaat cttcttttaa tccaggcatt
    17641 gccatagcaa gtattccccc ctcttttaca agaggtgata tatgattgtc taaaaatcct
    17701 tctttgtttc caaaatagtg ataagaatcg atacttatca ccgcatcaaa gaaaccttct
    17761 gcataaggta gttcatgtgc ctctgcctgt attggaaata ttttatcatc caatttaaac
    17821 tctttaaatc ttttatagtt ttcagttggt tctatccata aatctgttgc aaatacagta
    17881 gcatcaaatt cctttgctaa aaatatcgaa gatataccct taccacaacc taagtctaaa
    17941 atacgcattc ccttttctag ttttatctta cttgttaatt cttccaatat ttttatacaa
    18001 ttaggtccca tcatattttt catgagaaag tccttatcgt atttattact ttttgtaaat
    18061 atcattctat ctttcctttc cctatcagtt attatttatt aaattttata cgtttgattt
    18121 tttatttttt atctgcttct tatattgagc aatctctttt ttattcattc tttaacagac
    18181 aattttaatg tgtaatttta tccactctgt gatagtaact ttaatgaata atcttatcta
    18241 ctgtgatagt tattttagtg agtaatttta tctactccac agcaatcgct ttaatgagta
    18301 atcttatcca ctctatgata gttattttag tgagtaattt tatctactcc acagtaatcg
    18361 ctttaacgag taatcttatc tactctataa tagtcacttt aatgagtaat cataattttt
    18421 atatcatttt agatacttac tctaataaga attcattttc taatacttaa taagttttaa
    18481 ttacttccac tttaacctat atactaatta acttatttaa tttagtgcat atttgacact
    18541 ttacctttta ttataccatt tattatcata aatataataa ttctattttt ttaaaataaa
    18601 tatttataat aatctaaaat aaatatttat aaattctaat caaccgtaaa taataatcta
    18661 aaatacatca aaaaataaac taaaaaatat cgaaaaataa tttaaaagac atcaaaacta
    18721 aatcttctaa tcagcaatta aaagtctatt ttcttagttt aaaaaataaa gatagatttt
    18781 ttcaaaacac tggataaata ggtaaaaaaa taaacagtta gtaaaataaa tttttactaa
    18841 ctgtttattt ttattgttta atatgtccta taaatcctct aagtaatatt ttccttgcaa
    18901 aaattctgat tctaaaccta gatttttaaa ataatcaacc atgcccatac acgcaaattt
    18961 ttctgtagaa taatgagtag cacctataac attaattaaa ttatcttttg ctatttgatg
    19021 gaactctaat actggttcaa aatgttttaa aggcttagta aacccagtaa tatatagatt
    19081 tataccaagt tcagcaactt ctcttgctac aaatggataa cttcctcccc ctgctgcaat
    19141 agcaactaaa ccatctttta atacagaatc tccataatca tacaatttca catcatgacc
    19201 aacagatttt ttaattaatt ccttaatctc ttctattgat tttagtgatg ttttacatat
    19261 aactcctacc tcaattttat cgtcatattt acaaaatggt ttgactattt ctaaacccat
    19321 agcttttgca aaactaacag aagttgaata gtcactatag ttgtctagtg gagaatgtaa
    19381 tacataaaaa gaaattctac gatttttcat ctcttccata taagatagtg gtatgtcgtg
    19441 aaatggaaaa ccttcatctg atgctatgta tcccattgca tgatgtgaaa aaattaaaat
    19501 atctttttca tctttatcta taacttgctt aattatttct ttatcaggaa aagtagttgt
    19561 gtatacttta ttaatatttt gagcattatc taatactaat cctatgtatt tttttctaaa
    19621 ttctggagta ataaaatcat tttcaaaatt cataaaactc caatcatcat taatattttt
    19681 aatattaaat tctaaatcta attttttata aagttcaatt gccttaataa taaccactcc
    19741 tttataataa tattgaatta ctacatatta atatagaaat ttccgataat aaactttata
    19801 cgtttaa
//
LOCUS       DAYAOY010000076        19783 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYAOY010000076 DAYAOY010000000
VERSION     DAYAOY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.076"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            119..274
                     /locus_tag="ACGI6B_002787"
     CDS             119..274
                     /locus_tag="ACGI6B_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752221.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            404..1417
                     /gene="aroF"
                     /locus_tag="ACGI6B_002788"
     CDS             404..1417
                     /gene="aroF"
                     /locus_tag="ACGI6B_002788"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFG9752222.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1439..2503
                     /gene="aroB"
                     /locus_tag="ACGI6B_002789"
     CDS             1439..2503
                     /gene="aroB"
                     /locus_tag="ACGI6B_002789"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFG9752223.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2503..3816
                     /gene="aroA"
                     /locus_tag="ACGI6B_002790"
     CDS             2503..3816
                     /gene="aroA"
                     /locus_tag="ACGI6B_002790"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFG9752224.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3794..4873
                     /gene="aroC"
                     /locus_tag="ACGI6B_002791"
     CDS             3794..4873
                     /gene="aroC"
                     /locus_tag="ACGI6B_002791"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFG9752225.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4876..6072
                     /gene="pheA"
                     /locus_tag="ACGI6B_002792"
     CDS             4876..6072
                     /gene="pheA"
                     /locus_tag="ACGI6B_002792"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFG9752226.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6119..6928
                     /gene="aroE"
                     /locus_tag="ACGI6B_002793"
     CDS             6119..6928
                     /gene="aroE"
                     /locus_tag="ACGI6B_002793"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFG9752227.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6940..7473
                     /locus_tag="ACGI6B_002794"
     CDS             6940..7473
                     /locus_tag="ACGI6B_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFG9752228.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7494..8333
                     /locus_tag="ACGI6B_002795"
     CDS             7494..8333
                     /locus_tag="ACGI6B_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFG9752229.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8601..10082
                     /locus_tag="ACGI6B_002796"
     CDS             8601..10082
                     /locus_tag="ACGI6B_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9752230.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10115..13549
                     /locus_tag="ACGI6B_002797"
     CDS             10115..13549
                     /locus_tag="ACGI6B_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFG9752231.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13567..14313
                     /locus_tag="ACGI6B_002798"
     CDS             13567..14313
                     /locus_tag="ACGI6B_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFG9752232.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14262..14570)
                     /locus_tag="ACGI6B_002799"
     CDS             complement(14262..14570)
                     /locus_tag="ACGI6B_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9752233.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            15374..19516
                     /locus_tag="ACGI6B_002800"
     CDS             15374..19516
                     /locus_tag="ACGI6B_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HFG9752234.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         8056 a         2179 c         3293 g         6255 t
ORIGIN      
        1 gtaaataaaa aaatatttta aattgaaaat atttatagaa aatattgaca attatatttt
       61 ctttgttata attattaaac aagaagaata ataaattata aaaatggagg ataatactat
      121 gaaaggaata ttaaaagaac taacagcaaa actttatagc tttttttact ttatgttctg
      181 gggcttttat tttagcgctg gctatttctt ttacaaaaat atatgtactt cctttctggt
      241 gaggccttat tttagtgatt ttgagcataa gtaaatttta taaaaatgat tattttatat
      301 atttatataa gatttagtgc ttgatagatt attttagaga actcattagt gagttctctt
      361 ttttatttta aatataaaat tataatattg ggaggtagta aaaatgatag tagtattaaa
      421 aatgggagca gataaaaatg aagttaaaaa attaattgag gctattggga gagaaggagt
      481 agaagttaat cccatagatg gaacagaatt aactgtttta ggtttagttg gagatactag
      541 taaaatagat gccaaaagaa tagaagcaaa taaggtagtt gaaaaagtta tgcatgtggt
      601 agagcctttt aaaaaggcca atagaaaatt tcatccagaa ccttcaatca taaatgttaa
      661 tggaatggaa attggttcaa agaaaattgc tatgatagct gggccttgtt ctgttgaaac
      721 agaagaccaa atagtatcta tagctaaaga tgtgaaaaaa tcaggtgcag gatttttgag
      781 aggtggagca ttcaaaccaa gaacatcacc atacgctttc caaggattga aatatgatgg
      841 attggattta ttaaaaaaag caaaagaaaa aacagggctt cctatagtta cagaaataat
      901 gtcaacacaa gatatagata tatttgaaga aaatgtagat gtaattcaag ttggagctag
      961 aaacatgcaa aactttgatt tattaaaaga attaggtaaa acaaataaaa caatactttt
     1021 aaaaagagga ttatctgcta caattgaaga gtggctaatg tcagcagagt atataatggc
     1081 tggaggaaat gaaaatgtag ttctttgtga aagaggtata agaacttttg aaacatatac
     1141 tagaaatact ttggatttaa gtgcaatttt agctgtgaag aaacttagtc atttaccagt
     1201 tatagttgac ccaagtcatg cagcaggtaa atcatggatg gttgactctt tatctaaggc
     1261 agcaattgca gttggagcag atggtctaat aatagaagtt cataatgacc cagcacatgc
     1321 actttgtgat ggaaaacaat cgattaagcc aaatgaatat gatgaattga taagtgaatt
     1381 aaagactatc gcatcagcag ttggtagaga aatataatct ataaaaggat ggttaaacgt
     1441 ggaagaaaaa ctattagtaa atttaaaaga aaatagctac aatatattta ttaaaaaaaa
     1501 tatactaaag aatataggag aagaaataaa aaaaatatat tttggaaata agatttttat
     1561 tgtaactgat gaaaatatag caaaattata cttggataaa gtaaaaaata atttaagtga
     1621 atctggattt gaaattaaat ctttaatatt aaaatctggt gaagaaacaa aatcatttaa
     1681 tactcttcct aaaatatacg atagtttact tgactttaag ttaactagaa aagatttgat
     1741 tattacttta ggaggagggg taataggtga tttaggtgga tttgcagcag caactttttt
     1801 aagaggtata agttttattc aaattccaac atctttatta gcacaagttg atagttctgt
     1861 aggtggaaaa gttggagttg accttgagag aggcaaaaac ttagtgggaa gcttttacca
     1921 accaaaggct gtgtttatcg acccaaatgt attattatca cttccagaaa aattctatag
     1981 agatggaatg gcagaagtta taaaatatgg atgtataaaa gacaaaactt ttttcaataa
     2041 acttttaagt ttaaaaaata gagaagaagt aatgcaacat atagaataca ttatacataa
     2101 atgttgcttt attaaaaaaa ctgtagttga agctgatgaa aaagactttg gagatagaat
     2161 gcttctaaac tttggccata cattaggtca tgctattgag aaatactaca attttacagg
     2221 ttatacccat ggagaagctg ttgccatagg aatgtataag ataactttag aaagtgaaaa
     2281 gataggaatt acaaaaaaag gtacatcaga acttataaaa tcaattttaa tgaattataa
     2341 tttgccttat gagattgaat taagagataa taaatctata gtagatgcta tatctcttga
     2401 taaaaaaaat ctaggtgata cattaaaaat aatactttta aaagatattg gagagagtat
     2461 tatatataat actacttcta aattttttca ggaggtaaga taatgggaag tctaaaaata
     2521 tatccaagta aattatctgg agatgtaaaa ataccaccat caaaaagtat ggcacatagg
     2581 gcagttattt gttcatctct aagcaatgga aaaagtagaa tttctaatat agatttttct
     2641 gatgatataa tagctactat aagagcaatg acatcattag gagctattat agaaaagaaa
     2701 gaagatattt tagaaatttc gggaattttt tctaaagaag gtattttaaa tagagagaat
     2761 cagttgaatc aacctaaatt gacaatagat tgtaatgaat caggctctac attgagattt
     2821 ttagtaccta tatctttaac atttgatggt gtaaaaaggt ttataggtag aggtaatctt
     2881 ggaaaaagac cacttgatac ctattatgag atatttgaca gacagaatat aaaatatagt
     2941 tacaaagaaa atcagttaga tttgattata tcaggtaagc ttaaaccaga tgaatttaga
     3001 gtaaaaggaa atataagttc tcagtttatc acaggtttac tgtttatatt gcctacgcta
     3061 gaaagtgatt caaagataat aattactact gagctagaat ctaaaggata tttagattta
     3121 actctttcaa cgataaaaga ttttggggta gaaattataa acaataacta taaagaattt
     3181 attataaaag gaaatcaaac gtataaagcg agagattata aagtagaagg agattattct
     3241 caaggagcat tttatttatc agcagatgct ataggagaag atattagcat attagacctt
     3301 aaagaagatt ctcttcaagg ggatagtgaa gtagttgaaa ttttatctag gatgggaatg
     3361 gaaatattaa gagaaggcaa caaaataaaa ggaataacaa atggtttaaa tggtactttg
     3421 atagatgcat ctcaatgccc agatataata cctgtattat ctgtagtagc atccttaagt
     3481 atagggagga ctacaataat taatgctggt aggcttagaa ttaaagaatg tgatagactt
     3541 catgcaataa atgttgagtt aagtaaactt ggagcaaata tagaggagaa agaggatagt
     3601 ttgataatcg aaggagtaag caagttaaat ggtggagtag aggtgtggag tcacaaagac
     3661 catagaattg ctatgacact tgcaattgct tcttgtagat gtgataaacc aataatatta
     3721 aaagattttg agtgtgtatc aaaatcttat ccacattttt ttaaagattt taagatgtta
     3781 ggaggtcgaa ttgatgagtg gaatatgggg aaataattta aaagtatcaa tttttggaga
     3841 atctcatgga aatgctatag gtataaatat agatggtctt ccttcgggga ttgaacttga
     3901 ccttgataat attgataaag aaatgaagag aagagcacct ggaaaaaatt ctatatctac
     3961 ttcaagaaat gaaagtgata taccagaaat tctaagtgga tattttaatg gaagaactac
     4021 aggaacgcca ttatgtgcaa tcataagaaa tagtgatact cgttcaaaag attatggaga
     4081 gcttaaaaat ttaatgagac caggacatgc agatttcaca ggtaatgtga gatattctgg
     4141 atttaatgac tatagagggg gaggtcattt ttcaggtagg ataacagctc cattagtatt
     4201 ttgtggggct atatgtaaac aaattttatc acaaaaagga atagagatag gagcacacat
     4261 aaaaaaaatt aaaaatatag aagatatgag ttttgattat gtaaatattt caaaacaaca
     4321 gttatcaaat ttgcaaactt tggaactacc tttacttgat ttatcaaagg aagaagccat
     4381 gaaaaatacg attatagatg caaaaaatca aggagattca gttggtggta taattgagtg
     4441 tgcagtagta ggtattaatg tagggcttgg aaatccattt tttgactctg ttgaatctac
     4501 tttatcacat ttattgtttt ctgttcctgc tgtaaaaggt gtagagtttg gtttaggttt
     4561 tgaacttgca gatatgtatg ggtctcaaag taatgatgaa atgtattatg aaggaaatca
     4621 agttaagagc aaaacaaata acaatggagg aattatagga gggattacta ctggaatgcc
     4681 aataattttc aaagtggcaa ttaagccaac tccatcaatt agcagacaac aaaacacagt
     4741 taatataaaa gataaaaagg acgatatttt atatattaaa ggtagacatg acccttgtat
     4801 agttcaaagg gctatacctg taatagaagc agtaacagcc attggaattt ttgatttgat
     4861 gaaaggacga taataatgga ggatttaacg agatatagaa atgagattga caatatagat
     4921 aaagagctta ttcaattatt tgaaaagaga atgaatacag ttcttgaaat tgccagacat
     4981 aaaatgaaaa ataacactac tatacttcaa aaagatagag aagaaaaagt actaagtaga
     5041 gctgtagata atttagataa taaagcatat tcacaagaaa cggtacagtt ttttaattct
     5101 ataatggaaa ttagtagagg attacaaaaa agattgatag ataatgatgg tgagcagaaa
     5161 atcgagttta gtttagattc atcaaataag aaatttgatt tgtcaaatgt aaatagatat
     5221 aagtctttaa aagatgaatt aaataaaaaa aatatcctag taggatttcc aggaaaatca
     5281 ggttcattta cagaagaagc actaaataaa ttcttcaaca aaaaaatatc aaaaaaacaa
     5341 tttaaagaat ttgaagatgt tttcatagca cttaaaaata aagaaataaa ctatggaata
     5401 atccccatag aaaattcatc tacaggagct atctctgaaa cttatgacct tttgagaaag
     5461 tacggttttt atatagttgg agaagaatgt ataaaaatag accaaaattt aatagggata
     5521 aaagacacaa agcttgaaga tataaaagaa atatattctc atccacaagg tataggacaa
     5581 tgtagtaaat ttttaaaaca aaatagtaca tggaaattga taccatttaa taacacagca
     5641 acaagtgcag aacttgtaag aaaacttcaa gataaaacaa aagcagcaat tgcaagcaaa
     5701 aaagctgcaa gtatttatgg attagaaata ataagtcctt gtataaatga tattgcaaac
     5761 aactatacaa gatttgtagt aatcagtaat caaatacaca ttgaagaaga atcaaacaaa
     5821 atgagtgtag tattttctgt tgaacatgaa gcaggaaaac tttataaagt acttggatac
     5881 tttgctaaaa ataatataaa tatgacaaaa atagaatcaa gacctatgaa aaatgcctca
     5941 tggagatatt tcttttatat agattttgag tgttctatat acaatgctaa agtgtacgat
     6001 ttattagaat tgatagaaca taatacagca tattttaaat ttatgggagt atataaaaat
     6061 aaaactgaat agataagata aatttttatt taatttataa ttattaggag gtgtaagtat
     6121 gaattttttc ggtctagtag gtgaaaaact ctctcacagt gtttcacctc aaatccacaa
     6181 gagagtattc gagatattga atattgaaag tgcttataaa aactttgaaa tttcaaaaga
     6241 agatatatct aagctagacg gtgctataaa attattagga atacaagggg taaatgttac
     6301 tgtaccatat aaggagcgta ttatgaaata tcttgatttt atatcaccag aggcaaaacg
     6361 tataggagca gttaatacaa ttttacttag agaaaatatg ttatatggtt acaatacaga
     6421 ttactttggt cttgatagta tgtttaaaat ggctaatatt gatgttcaag gaaaagtagc
     6481 agttatatta ggaactggag gtgcttctaa agctgctctt acatatttta ttgattctgg
     6541 tatagaaaag ctttatgttt caactagaaa aaaggatgat aaaaaattat taaatagtaa
     6601 agctatatta atagattatg aagaacttaa acatataaaa ggagatataa tattaaatgc
     6661 tacaccagta ggtatgtacc ctaatgtagg aatttcacca gtgtcaaaat ctattataca
     6721 aaattttgat atattaatag atttaattta caatcctgga gaaacagagt ttttaagaat
     6781 tggtaatagt atgggtaaaa aaacttgtga tggattatat atgctggtgg ggcaagcaat
     6841 aaaatctcag gaaatttggc aagatactaa aatagataat agtattttag atgttatata
     6901 taatgaacta aaattagaat ttttgtaagg gagattgtta tgataaataa aacaaaagaa
     6961 aaattgatat taataggtat gccaggtagt ggaaaaacta caattggtaa acttttggcc
     7021 aaagaatata attgtagttt ctgtgatatg gatgattata taattcaaat atctcaaaaa
     7081 agtatagcag agttgttttc agaaggagaa gatatcttta ggaactatga gactcaagca
     7141 tgtagagaac tttctataag tgataaaaca gtgatatcta caggtggagg tgtaataaaa
     7201 aaggatgtta atatggaaat actaaaagaa acaggtataa taatttttat tgatagacct
     7261 atacaaaaga ttttggaaga tataaatatt aattcaagac cattattaaa aaacggaaaa
     7321 gatagattgt ataatcttta taatgagaga ataaatcttt ataaaaaatt ttcagatata
     7381 gagattttaa atgataaatc attaaataat gctgtatata atattacaaa tgcagtcagt
     7441 gaaaatttta aatttgactt taaagaaaaa taatctaggg ggaaataaaa ataatgaata
     7501 ttgttattgt aggactagga gttataggag gctcttttgc taaagcatta aaaaaagctg
     7561 gatatgaaaa tgtatttgga gtagatgtag atttagaaac tttaaaaaaa gcagaaaaag
     7621 caaaaatcat aaaaaaagga tgtactacag gtaaagagtt atttaaaaaa gctgatttaa
     7681 ttatattatc tatatatcct agattagttg tagattttct aaataataat aaaaactttt
     7741 ttaaaaaagg gactataatt actgatacta caggaataaa agaaacactt atcaatgatg
     7801 tgttgcaaat tattccagat gatatagatt ttatatttgg acatccaatg gcaggtaggg
     7861 aaaaaaaggg tattgatttt gcaagtgaac aagtttttaa tggcgcaaat tatataatta
     7921 ctccaactgg cagaaacaac ataaaaaatt tagagttagt tgaaaattta attttagaga
     7981 ttggatttaa aagagtaaaa aagttaactt cacaaaaaca tgatgagatt atagctttta
     8041 cttctcagtt acctcatgtt atggcagttg ctcttataaa tagtgatgaa gaaggtagag
     8101 atacgggtaa atttattgga gatagttata gagatttaac tagaattgca aatatgaatg
     8161 aagacttatg gagtgaactt tttttaggaa atagagataa tttactaaaa gttatagaaa
     8221 actttgaatc agaagtaaat ttagtaaaag aagctatatt taacaatgat aaaaataaat
     8281 taatagagta ttttaaaaaa tcatcaataa gaagagaagc tttagaaaaa taaatattaa
     8341 tatttaagct atatatagat gggtataaat ataagcgata gtgtatttaa ttgtattaat
     8401 aaatatggat tcaatataat taaatatgct attgatatat ttgtaccctt aactattgtt
     8461 atcttttaaa tgataatgat atttatttaa atactcttta ttaacaattt ataaaactta
     8521 attgtaaaaa atacttatta atagtaaaat aaagtgaatc aattatatta taattgctaa
     8581 agagagtgag gtgtagctat ttgaaaaaaa atagattaat agcaaagaat atatcaatag
     8641 catttatagt actattcttt tttagtgtat ttacattttt ttatgtaggt aatattaata
     8701 gagtgttaga atatgaaaca aatgacataa taacagttac aattgctgga tggattatac
     8761 tttcattttt atttttaggt attataattt acatattata ttcaaaagca aatagtcaaa
     8821 aaactataga aaaagtagca tatacggatt ttgtaacagg ttattccaac tggagaaagt
     8881 ttgaattaga tgttacaaat ttattaaaga aaacatcaca aaataataaa tatgcaatgg
     8941 tcatattcga tattgataaa tttaaagcaa ttaatgatat atatggccat aaaaagggaa
     9001 atttaatatt aaaagatatt gcagatactt taaatgaatt gactgatatc aatgaaactt
     9061 ttgctcgtgt aagtgctgat aatttcaata tattattaac atataacaaa aaagaggata
     9121 taataaatat tatcaaaaag attatggcaa ataatgaact tgtaaactta tcttttggaa
     9181 tttatgaaat caaagataag gatttatctg taagtgtata tagtgataga gcttcacttg
     9241 caaaatcatc aataaaaaat aatagtgatg taaattttgc attttttaat gacaaattaa
     9301 gagagaaatt attatttgaa gataagatag aaaaagaaat ggaatatgct cttgaaagtg
     9361 ggcaattcgt aatgtattta caaccaaaat acaatattaa attagataaa ttttgtggaa
     9421 gtgaagccct agtcagatgg caatatacag aaaaagaggt catatatcca ggtgatttta
     9481 ttcctatatt tgaaaaaaat ggatttatta gaaagattga tatgtacata ttagaacaag
     9541 cttgtaaaga aatccggtct ttatttgata aaggaatttc acctttacct atatctgtaa
     9601 acttctcaag agttgatttt tttaaaaaag attttattga aaacatagta aatatatgtg
     9661 atagatataa aataccatat agtttaatag aaattgaaat tactgaatct agtatgtttg
     9721 gagatactga tactctattt aatgttagta gaaacctaca ggatattggc tttatagttg
     9781 caatggatga ttttggctct ggatattctt ctgtaaatat gcttaaaaat ataccattaa
     9841 atgtaataaa attagataga ggattttttg ttgatgataa agatgttgat aagagtcaaa
     9901 ttgttataaa aagcatagtt tctctcatta agcaattagg aataagggtt gttgctgaag
     9961 gaattgaaac aagaagtcaa atagaaatgt taaaaaaagc aaattgtgat attgttcaag
    10021 gatattattt ttcaaaacca ttaccaatta aagaatttga aaaattagtc tataaaatat
    10081 agtcagcgag tcttaaatgg gaggtggtga ttaagtgata agagcaaaat cgttaaaaaa
    10141 tgtactgagt attgttttaa tatttttatt tacaataatt tctttttctg attttgcatt
    10201 taaaactaat gtccaggaag aagaaaatag taaaattaat ttaagagaaa tatccctaca
    10261 aagttccaat gctataaaaa taaaattaga tagttctcta tcctttatta aaagtgtggc
    10321 aaatatggtt tcttatttta atactataga taactcaaaa atagttccat tattagctaa
    10381 tatatcaaaa gaatctgact ttcaaagaat ctctattgta tctcctgatg gaataagtca
    10441 taccagtgat ggcaaaatta tcaatgtgtc aaagtatcag tattttaaga aagctattaa
    10501 aggagaaagt aatatatctg aagttgtgaa atctccaatt gatggaaaag atacttttgt
    10561 tatagctact cctgtattta ataagaataa caaaataatt ggtgttttat acggttctta
    10621 ctacaatgat aaattagctg aatttataga tataacaagt tttaatagtg aagggtacat
    10681 agatatattt gaatctagtg gtcaatttgt tttaaaatct tcacatacaa acaaactgat
    10741 taagtcaagt agtaacatat atgatatatt taaaaaagct tcttttgatg atggttatag
    10801 ctataaaaaa atatatgaag atacaaaaaa taacaagagt ggctttacct catatgaata
    10861 caaaggagaa aatcgttatg ctacttatac acccattggt gtaaatgact ggtatgtaat
    10921 ttctgtgata ccacaatata ctgtaagaag aaacattgat aaaataaatc aattagcatt
    10981 ttctcttatt ttcaaggtat tcttattgtt tattatgctt ataatatata ttatttataa
    11041 aaacaataaa tctcagaaag atatactaaa agctaacaaa gaaatctcat ctattacaaa
    11101 tagcattcct ggaggagtac aaaagtgtac cctgggtgaa acatgcaata ttaatttttt
    11161 aagtgatgga tttatagagt tgtttggtta tacaagagaa gaaataaaaa cattgtttaa
    11221 taatgatttt tatagtttaa tacataaaga tgatgttgaa agaataaaaa aagaaatttt
    11281 taaacaactt tacaaaaata aaactgttga attagaatat aggattataa caaaaaatgg
    11341 taatacagta tgggttttag ataaagggga gcttataaca gaacctgatg gaaaagaata
    11401 tttttgttgt gtaactgtag atattactag aacaaaacaa ttagaacaag agatggaaca
    11461 aaaaaatcta gaacttgaaa tgatatcaga aaatgtttta ggtggtataa ttataacaga
    11521 atttaatgat gattttacta taaaatacgc taactctggt tatctttcaa tgattggata
    11581 tacacgtgaa caattagaaa atgaacttaa atacaaagct tgtaatcttg ttttttatga
    11641 ggataaaaag aaggctcttg aaagtataca acatcaatta aacacaaaag gaatagctgt
    11701 atatcaacat agaataagaa gaagaaatgg taatattatt tgggtttcta taaaaggaag
    11761 gcttattgtt gatgatggta ttgaaaaagg tatttggatt ctaaatgata taacagattt
    11821 aaaaaatgca gaacaaaaac ttaaaataaa tgaagaaaga tttagaattg cattaaagca
    11881 aacatcaaac actatatttg agtatgatat aaaaacaaag tcacttttaa atactagtaa
    11941 agagcttgat aaatataata tttataaaat tactaaaaac gttccatatt ctctagtaga
    12001 atcaggagtt attcataaag attttagtga tgattttata aacatgtatg aaaatgtaat
    12061 aaatggagca aaaaaagcaa gttgtgtagt taagatgaaa cttccagatg aaaattatat
    12121 atggaataaa gttaccttga ctactatttt tgatgaagat ggtaatcctg taagagcaat
    12181 tggtatacag gaagatataa gcaaacaaaa ggaagcagag ataagataca atcaagaagg
    12241 taagtatcat tcagctatat tgtcggaagc aatgataact tatgaaataa atgtatcaaa
    12301 ggatactttt atcaaaggaa atgaaagttg gcaaaaacca tttggaatta agcctgacaa
    12361 taactattct caaatggtaa aaactatagc aaatagtatt gtacataaag acaataaaga
    12421 aacattttta aatacatttt tatgtgaaaa cttattggat gcatataaaa ataatgtgac
    12481 tgaaataaaa ttagaatatc gaagaatgaa tcaagaaaat aaaatgattt gggtcatttg
    12541 tacaatgcac ttaataaaag attctattac gaacgatata aaagcacttg cttatgttaa
    12601 agatattgac aagcaaaaaa atgaagaaat tagattaaaa ttaaaagctg aaagagatac
    12661 tcttacagga ttatataata aaggtactac agaaaaattg attaatgatt tcttgaactc
    12721 tgaggaagca agcttaggaa tgcatgcatt tttaattgtt gatatagata attttaaatc
    12781 tgtgaatgat aatttaggac atgtatttgg agataaagta ttatttgaag tttctaaaaa
    12841 cctaaaacct atatttagaa aagatgatat agtaggtaga attggtggag atgaatttat
    12901 agtattctta aaaaatattc agtcttttga gaatatcaaa aagaaagcag aaaaaatatg
    12961 cgatatattt agaaattctt atacagaaaa taaaaagaca tataaaatat ctggaacagt
    13021 tggaatttca tttgtaccag aacatggaac tgtatttgaa gaactttata aaaaagcaga
    13081 catagcactt tattatgcaa agagcaaagg aaaagataag tatgcacttt ataatgattg
    13141 tatctaccaa aatgaacttg aaaataatat aagtgataat aatataaaat tagcagataa
    13201 atgtgagaaa tccaaaccag tacttaaaga aattcttgat tctattgata attatatcta
    13261 tatagtgaat ccaaatactt atgaacttct ttttgtcaat aataaggtgt ctcgtttgga
    13321 tttagagata aaattaggag aaacctgtta taaatcacta ttaaaatctg atagaccatg
    13381 taataattgc cctcttaacg gattagaaga aaataagcat tcaaaattca aaatagagac
    13441 aaatacattt ggtaaaaata tcagtatagc aagttggatt aattgcatag atggagaata
    13501 caactgtctt atagagtgta tagattcttc tttatacaaa aataaataaa aaaggactat
    13561 gaagatatgt taaatagtat aactttgata cttggtataa tatttacaat ttactatttt
    13621 tatctcaaaa tagtttttgg aggaatttca tttagtgaga ttttcttagt tgtaggaatt
    13681 gtactaatta tctatcaaat atttaagaaa aaaattaaag aaaagaaggt tttttatagg
    13741 gtgttaaaaa taataatcag cattttttta atagtatttg tagttactga gagtctaatt
    13801 attttttatc ccaaaaatag tttagaatca aaaagtgatt atctcttaat tttaggagct
    13861 tctgtaaaaa agactacacc aagtacaacc ctgaagggta ggttagacac tgcattgaaa
    13921 tacctaaaaa ttaatgataa ttgttatgtg gtagtatctg gtggtaaagg aagtggagaa
    13981 aaaataactg aggcaaaagc tatgaaagat tatcttataa aaaatggtat agataaaaat
    14041 aggataatag aagaggataa atccacgaat acttatgaga attttaaata ttcaaaattg
    14101 aagatagaag aacatagtca aggaaaacta agcgatttaa aagtaaaaat agtaacaaca
    14161 gattttcatg ttctcagaag taaaatatta gcatatagaa atggatataa aaatacgtct
    14221 ttttatgcta gtaaatcaaa actttcattt gtaccaactt actatacaag agaatttttt
    14281 gcattatgga aaacaatcat ttttgataga taataaataa aaagactaga tataaactca
    14341 tatctagtct tttctattcc cttaattcta atccattttc caactttact tgttgtagaa
    14401 atttcaacat tgggatttac aagtaattgc ttataagaag ttttatattt tacgattcca
    14461 aaatataatt ttccttcata atccattata aatccgaatg gtcttactct tggtttatta
    14521 tcttcaactg tagcaaaata gaatgttgag ttttctttaa aaaaattcat tattttttca
    14581 tgattaaatc ctctttgtat cttatactaa tataatgaat ataaataaaa tattcataat
    14641 actaatataa atcgatataa aaacaattac aatattatat tgattattca aaattatata
    14701 taaaattcag aaaaaaattt tagtttacat aataaaaata aagtcaacta aaattattga
    14761 ttttagatta aagtaccgga ttaagcattt aagtagattt taagcttgat ttgattagaa
    14821 aggtacaaac tatcaccgaa aaaaattaaa cccatttaaa aagcaatata aaagcttgtt
    14881 atttttgaga aaaatagacc ctctaaactc aattagatta aaaataaaat acagaaaatt
    14941 atatctctaa atatcttaaa taattaagtt taaattttac gatattttgt ttgatgattt
    15001 ataatataaa agcatactct taatcttcaa aatttaaaaa gagtaaaaaa taacaaaaaa
    15061 atcactgaca tttcactgac aaattgtaat tttgttatta taaatacatt gaaaatactt
    15121 gattattgaa aaatggcata aaataataca ttttatgcca tttgctatta tttgatagtt
    15181 tttaacttat gacataagat attttttaac atatacaata aaatataaaa aaattgatat
    15241 tgcaaataaa aaaaaataca tattagatgt tatagaaaaa aagcgcactt ataggcttaa
    15301 aatgtcacat aaaagagttt taaagaataa tatattatga acacaaattt tgtaatcaca
    15361 aaggagtgat aatatggcaa cagtatcagg acaaatagtt tttgatagaa atagaagtgc
    15421 taccattgat tctggagact caggaatagc caatatacca gttgtattac aagatatagc
    15481 aactggagtt agacttgtgg ttctcacaga tgcatcagga aattatgttt ttactaatgt
    15541 tccaaatggt aactatagaa ttgtagaagc ttttggaact acaggaggag taccaacacc
    15601 aggaaatttt gcaacagcga tagtaggtcc agttccaatt gcagaaatgc cacctattag
    15661 ttctgtgaca agtcctccta ttggttcaac aaacttagat ggtacaacac ctaacacact
    15721 attaataaca gtagcaggtt cagatataac aaatcaaaat atattaaatg ggcctgtgat
    15781 ttatacacct atagagaata tattggattc ttgcgtatct gtatctaata taaaccttat
    15841 tacagatgca gataacggaa cattcggttt ttttccacca ggtacaccaa gtaatacagg
    15901 acctgaaata gaaccttacc caaatgtgac gccagatttt acgtatgttg taccagaccc
    15961 aactaagttt acacctttag atggagaata tacagtacaa aatattatga caaatgcaat
    16021 gagtaatgtt ataggagctt ggtggcgtat tgctgaccat actacaggaa atgaaacagg
    16081 aagaatgatg gtggtaaatg gttttaatcc tggagctgta ttttttaaat caacagtaca
    16141 agttacacct aacactaatt atttatttag cacttggatt ttaaaccttt ttaaggtaac
    16201 aggatatcca ccaccacagc taggtgtaag aatatttgac caaaataata atatactata
    16261 tcaagcaaca ttaggagctc aaattccagt taatacaaac gcaccagaat ggaaagaagt
    16321 aggtactgtg attaattctc aaaataataa tcaacttaca gtagaatttt ttagtgaagg
    16381 agaaagcgcc attggaaatg actatgctat agatgatgtt tctttaagag aaatagctgt
    16441 tccaactttt actccaataa aaacaagtaa cacagaaact gctaatgtag gtgatataat
    16501 cacatataca gttacattag ataatacttg tgatagccct ttaactaatg tattttttag
    16561 ggatttaatt ccaaatggtt tagagtttat acctggaact gtaacggttg atggagtatc
    16621 tgtaccagga gtagacccca atacaggttt cccacttcca gatattggag gaggaacagg
    16681 ggttgaagta acttttgatg ttgtggttga aagtattcct aacccaaatc ctactaataa
    16741 tatagctact atagattact cctatacacc tgtagaaggc ggtataccaa atgatttttc
    16801 agtagattct aatccagttc ctgttgaagt aatctcagca gatattgaag ttacaaaatt
    16861 atctgaacct actatagtga atccaggaga ggaattaata tacactataa aagtagtaaa
    16921 taatggacca ttcccatctg aaaatgtggt tttaacagat gatgtaccag cttctattgt
    16981 aaatccagag tattcattag atggaggagt aacttttcaa ccatggactg gaagtttaaa
    17041 tatagggaca ttagaagtag gagagacaag agttattata attaggggta ttgtaaatcc
    17101 atcaacagta ggaattataa caaatacagc tgtagtatct tcaactacag cagacccaaa
    17161 ccttaataat aatacatcta caatagagac agaagtgaat ttattagcag atattttagt
    17221 aatgaaaaca gcagaaccta attcagctgt tccaggtact ttattgagat atactattca
    17281 agtagaaaac ttaggaccag caaatgctga aaatgtaata ttaaatgatg atattccagc
    17341 ttctattata aatccagaat attcattgga tggaggagca agttttcaac catggaatgg
    17401 aagtcttaat ataggaactt taaactctgg aatttcatta acagttttaa ttcaaggaac
    17461 agttagttta aattcttcag aatatatagt aaatacagca acagtttctt caactacacc
    17521 tgacccagat ttaagcaaca atatatctac cattattaca ccagttaacc cacaagcagg
    17581 tatttctatt ataaaagtag ctgacgagga tgtagctgtt ccaggagaag aatttgtata
    17641 tacaattgaa atttttaatg aaggaccatc taatgctacg aatgtagttt taacagatga
    17701 tataccagat gttattttaa atccagaata ttcattggat ggaggagcaa ctttccaacc
    17761 atggactgga agtcttaata taggtacagt tgcaccaggg caattaataa gaattatcat
    17821 aaagggatta gtaagttcaa ctgctacagg agatattact aatacagcag aagtaagtgc
    17881 agatgtacca gaaccagtta cggattcatc aacagtgaca actcctatag ttccatcagc
    17941 agatatagaa gtaataaaaa ctagcaatat ggatacagca gttccaggag aaactttcag
    18001 ttatactata acagttacaa atttaggacc atctgctgca caaagtatcg ttttgacaga
    18061 tgatatacca gatgttattt taaatccaga gtattcattg gatggaggag taactttcca
    18121 accatggaat ggaaatctta gtataggcac tttggatgct ggagaaataa gaagtattat
    18181 aataagagga acagtgagcc agacagcagt aggtacaatt ataaatacag caacaataag
    18241 ttcaactaca cctgacccaa atccagataa taatacttca acagatgaaa cagatataag
    18301 tccattagca gatatttctg tgataaaagg caacgaacca gttgctatac cagggggaag
    18361 atttatttat ggaattgaaa ttgcaaatgc aggaccatct tttgcagaga acgtaacttt
    18421 aacagataat ataccagctt ctattttaaa tccagagtac tcaatagata atggagtgac
    18481 tttccaacca tggaatggaa gccttaatat aggtacttta gatgctggag agataagaaa
    18541 tatcataata aggggaacag taagtcaaac agcaataggc acaattataa atacagcaac
    18601 agtaagttca actacacctg acccaaatct aaataacaat atttcaacat cagaagcaga
    18661 ggtaagttca tcagcagata tttctgtagt aaaacgaagc aatcagactg ttgttgtacc
    18721 aggagatgta ctagactata caattgaggt gagaaatgca ggaccgtcta ctgctcagaa
    18781 cgtaacttta acagataata taccagcttc tattttaagt ccagaatatt caatagataa
    18841 tggagtgact tttcaaccat ggaatggaag tcttagtata ggcactttag atgctggaga
    18901 gataagaaat attataataa ggggaatagt aagtcaaaca gcaataggta caattataaa
    18961 tacagcaaca gtaagttcaa ctacacctga cccaaatcca gataataata cttcaacatc
    19021 agaaatagat ataagttcat cagcagatat ttctgtaata aaattagcaa ataaaacaga
    19081 agcatgtgta ggagagcaaa tagattttgt aatagtagta tcaaatgcag gaccagaaaa
    19141 tgctcaaaat gttactttaa tagacaatgt ttcagataag cttaagaaag cagtattttc
    19201 acttgataga ggtgtaagtt tccaaccatg gactggaagt cttaatatag gtacactacc
    19261 agctggaact ttaagagtga ttatattaag aggaattata aaatcaacat gtcttgatag
    19321 attaacaaat attgcagaaa ttacttcaac aacaccagat tcaaacctta acaataatat
    19381 atcaagagtg caagtagaaa taaaacaatg ttgttgtaat gattgctgtt gtaacaactg
    19441 tagtaattgt tgttgcaatg attgtggttg taaagattgt tgctgtaatt gttgtaaaaa
    19501 tgacttttgg tgttgatttt ataaaataat tttgtatacc ttaaattaaa ctgaaataaa
    19561 aaataaattc acatatgagt aaaatatttt ataaatatta ataaattttc tatctttgat
    19621 atggaccaca ttcgtaatac tagttattaa atgaagtggt cctttttaat tcattaaaat
    19681 tatttattct aattaaattt taaatgtatt aaataatttt aattttttat aaaaaatatt
    19741 catatgttat ttatattaaa gtttagatat ctaaaaatac ttt
//
LOCUS       DAYAOY010000077        19678 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYAOY010000077 DAYAOY010000000
VERSION     DAYAOY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.077"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            227..748
                     /locus_tag="ACGI6B_002801"
     CDS             227..748
                     /locus_tag="ACGI6B_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752235.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            782..1558
                     /locus_tag="ACGI6B_002802"
     CDS             782..1558
                     /locus_tag="ACGI6B_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFG9752236.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1900..2853
                     /gene="gpr"
                     /locus_tag="ACGI6B_002803"
     CDS             1900..2853
                     /gene="gpr"
                     /locus_tag="ACGI6B_002803"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HFG9752237.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2871..3890
                     /gene="spoIIP"
                     /locus_tag="ACGI6B_002804"
     CDS             2871..3890
                     /gene="spoIIP"
                     /locus_tag="ACGI6B_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HFG9752238.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3921..4172
                     /locus_tag="ACGI6B_002805"
     CDS             3921..4172
                     /locus_tag="ACGI6B_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752239.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4454..6259
                     /gene="lepA"
                     /locus_tag="ACGI6B_002806"
     CDS             4454..6259
                     /gene="lepA"
                     /locus_tag="ACGI6B_002806"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFG9752240.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6408..7745
                     /locus_tag="ACGI6B_002807"
     CDS             6408..7745
                     /locus_tag="ACGI6B_002807"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFG9752241.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7978..9291
                     /locus_tag="ACGI6B_002808"
     CDS             7978..9291
                     /locus_tag="ACGI6B_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9752242.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9463..10638
                     /gene="hemW"
                     /locus_tag="ACGI6B_002809"
     CDS             9463..10638
                     /gene="hemW"
                     /locus_tag="ACGI6B_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFG9752243.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10741..11760
                     /gene="hrcA"
                     /locus_tag="ACGI6B_002810"
     CDS             10741..11760
                     /gene="hrcA"
                     /locus_tag="ACGI6B_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFG9752244.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11790..12410
                     /gene="grpE"
                     /locus_tag="ACGI6B_002811"
     CDS             11790..12410
                     /gene="grpE"
                     /locus_tag="ACGI6B_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFG9752245.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12502..14349
                     /gene="dnaK"
                     /locus_tag="ACGI6B_002812"
     CDS             12502..14349
                     /gene="dnaK"
                     /locus_tag="ACGI6B_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFG9752246.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14560..15714
                     /gene="dnaJ"
                     /locus_tag="ACGI6B_002813"
     CDS             14560..15714
                     /gene="dnaJ"
                     /locus_tag="ACGI6B_002813"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFG9752247.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16680..17615
                     /locus_tag="ACGI6B_002814"
     CDS             16680..17615
                     /locus_tag="ACGI6B_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9752248.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17651..19024
                     /locus_tag="ACGI6B_002815"
     CDS             17651..19024
                     /locus_tag="ACGI6B_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9752249.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19212..>19678)
                     /locus_tag="ACGI6B_002816"
     CDS             complement(19212..>19678)
                     /locus_tag="ACGI6B_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HFG9752250.1"
                     /translation="VTTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHII
                     KDNAENKFLNLFDFIRYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLN
                     ISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         7798 a         2165 c         3554 g         6161 t
ORIGIN      
        1 aatttatatg cttaattaag ataatttttt tcattagtat gtatgataga tgcatcaaac
       61 aatataacta atatgttaga aggttatatg taattaatgt cagtgtttat atagaatagc
      121 atttgtagca tttattatag ttgtaattac tatttatatc atagacttga atagtctaaa
      181 aaaatagact ataatatttt tatagactaa agaaagggct gataagatgg acatagcaaa
      241 actaatttta aatcctgcaa ggcttcgtat tttacaatat attcgacttt atggcagtgt
      301 tcgtacatct gatattgtaa agtatttaaa tgatattcct cgtgcaacag tttatcacca
      361 tgtaaaaatt ttagaagaga acaatatgat tgaagtaatt aaagaaaatc gtgtgcgtgg
      421 tactattgaa aaggtttatg cactaaagga atatacaaca tctatggaag gagagacctt
      481 cgttgcactt tctacagcat tttatgtagg tctgatgcag gaaatgaatg aatactttag
      541 cagaaaagag caagaccata aaaaggataa tgtatttttc tcttcagcat tactatatat
      601 gagtgataat gaatatgaaa atcttcttaa aagtatagct gatttattga aaccatatat
      661 agaacaaaaa cctaaatcaa atctaaaact tagaaaactt tcaattatat cttctccacc
      721 tgtagctgat aatgataaaa caaaataaca tgcaacccat ttttataaaa ggaggttata
      781 tatgaaattt catgagtttg gaaataaaaa cagaccacat attatgttga ttcatggggg
      841 agggaatgca tggtggaact atttgcgaca agcagaagtt ttgtctaaca aatatcatgt
      901 gatacttcct acattggatg gtcatggaga agaatatgaa acaacataca tttctacaga
      961 ggatacagca gataagttga tatcatatat agatgaaaat tgcagtggac atcttttcgc
     1021 tttgtgtggg gtatcacttg gtggtcaaat tgtcatggaa cttttatctc gaaaacctaa
     1081 tattactaaa aaagcaatta ttgatggtag tatttgctac ccaagacctc ttatggcaag
     1141 gttttgtatt gctggtattc gattttttgg aggacttttg tttagtgaga aggcttgtcg
     1201 ctttcaaatt gctatgatgc caaaattgtt acctgaaaat atgcaatatc cagataaaat
     1261 aaaagcgtat tatatgacag atatgccaca tgtaagaaaa gaaacattct ataatatgta
     1321 tcgtacttac atgatgaact atacacttaa agagaatgtt aaaaaaacca cagcacaagt
     1381 aatgtactgg tatggagaaa aagaaatgaa gtgtgtcaaa aaatctgcaa aactgtttca
     1441 gtcttatgtg ccctcttgtg agatttatga agctaagagc tataatcatg ggtatttatc
     1501 actttatctt ccaaatgaat ggttggaaat tgcagaacca tttttccaaa aggaataaag
     1561 gtgttaggtg attattgtat aattattgct gtttttttat atagtaaata aattaggtat
     1621 aaatataaaa aattatttta gttaatatat tagtgaaagc ttaataggaa aattataaat
     1681 agattaaagg tagcacctat ataaatagtt gctacctttt tctgtttgta aataaatgat
     1741 agataagaag gtttactgac aaatcactga ctaaacccaa ttttcttaca ttgaaagcct
     1801 taattttact tgttttaaat aaatttacaa tttcttgtta taaaatacta aattttgtta
     1861 acaattataa ttagcattta atttatactg gaggtttata tgattagtgt aagaacagat
     1921 ttagctttag aagctagtga aatgtgtgaa aagtctcaag aaggaagtag tattcctggt
     1981 gtaaaaatag aaactaaaga attagaaaat tgcattgtaa ctaaagttga agtaatagac
     2041 aggcaaggcg cagagataat gaataaagat ataggaaagt atataactct ggaaagtaat
     2101 cttatgaagt ttgatgatga tgagtcaaga gaagaaatga taagttattt aaaagaggag
     2161 ttagtagata tatttggcca agacaaaaat aaaaaaaccc tggtaattgg tcttggaaat
     2221 agaaatatta cttctgatgc gttaggacca aagagtgtat caaaaacact tgtaactaga
     2281 catcttttta aaaattataa taaagactat gatgatgatt tcactgaggt atcagcttta
     2341 agcccaggag ttatgggtgt tactggaata gaaactagtg aaatagtaaa atcattggtt
     2401 gagaaggtta agccagatag agtagtagca atagatgcat tggcttcaag aaaaatggaa
     2461 agagtaaact ctactataca gatatcaaca gcaggaatat cacctggtgg aggtgttggc
     2521 aatactagaa aatctctaac taaagaaact ttaggcgtgg atgttattgc aataggcgtt
     2581 ccaacggtgg ttgatgcagc aactcttaca atagatgtct tagatatggc aatagacaat
     2641 ttaatagctc aatcagaaga aacagaaagt ttttatgaaa tgttgaaaaa acttaaagaa
     2701 gaagaaaaat atcatctaat aaaagattcg ttagacccat atgacaagaa cttaatagta
     2761 acaccaaagg atatagatga tacaatcgaa aatctatcta taataataag tgaaggctta
     2821 aataggtctt tacatccagg tagattggta taaaataagg aggagcaatt atgtttaaaa
     2881 aatgcattaa agtagtaacc ttgactttta tattagtatg cattttacca ggaaaatcat
     2941 tagctttgaa tcaagatgat tttttaaagt ttttagtaaa ttcatcttat ccagaagcta
     3001 aagtcgaagg aaatgataca gaaaataaaa agaataataa aaataaagaa acaagtaagg
     3061 aaaataagga agaaagtaaa gaaggaaaca ctaaatctaa agatgcaagt aaagttgata
     3121 ataaaaaaga gagtgaaaaa gaatatataa aactttatgt tggtaaagaa aatgttccag
     3181 atattgagtc aaaaaattca gacacaactg aaactaacac aacatctagt tcagattata
     3241 aggatgattt aagagtaacc aaagaaaatc caagaatttt gatatatcat actcatggat
     3301 gtgagactta ttcaaattca cctgatggaa attatcattc aagagataag aaaaattcag
     3361 taatggaagt aggaagtgca ttaactagtg ctttagatag caaaggatgg ggtgtagtcc
     3421 atactactaa ataccatgac tatccatctt ataataactc ttatgcaagt agcttaaaaa
     3481 caatacaaag tatattacct aaatataact cagtggatat agcgatagac ttacatagag
     3541 atgctaggga tttaactaat ccagccacta aggaaaaaga ccatttaaaa tatacaacta
     3601 tgataaatgg agagagagta tcaaagttct tctttgttgt aggaggtaaa aatacaaata
     3661 gaaaacaact tagagcttta gcagaagata taactgcttt tgctgagaaa aagtatcctg
     3721 gtctagtatc tccaatagta gaaaaagact atgctagatt caatcagttt gcagttaaga
     3781 atcatatgtt agtagaaata gggaataacg caacaagtgt agaagaatct aaggctacta
     3841 caaagtattt agccgagatt ttggatgaat attttaaaca aaaaaattag agctttaagt
     3901 tataattagg agcacgctaa atgagccgat tagaaaaaaa tatagaaagt aaaagtaaac
     3961 taagaaaata caagtatatt atgagaatct tatttttgat tactatgatt atatgtacat
     4021 cagtttgtgt atttatagtg gacagtaatg caaaaagtat gctaggaatg aaacaatatg
     4081 attttccaag tattaaattt gatataacaa aatttgattt tgacatacca aattttgata
     4141 tacatgaaat cataaaaaaa tttaataagt agtaataagt gaataaggtt tattgattaa
     4201 aataagcctt attttagtta tatattttta atgtataaaa taaaaattca ttaactgtat
     4261 tttataggat ttactttaag tttatgatat tatataatat ttagtgttag acttgataat
     4321 ccacatataa tgtggataat tttgtttagt gccaattagt ttatattcaa aaattttaaa
     4381 tttggtatat aaagagtttt tagataatgt aaaataataa ctataagaat aatcaagagg
     4441 agggaatttt aaggtggaca ataaacaaag tagaactaga aattttagta taattgcaca
     4501 tatagaccat ggaaagtcta ccttagctga tagattaata caacaaactg ggcttgttag
     4561 tgaaagagac atgaaaagtc aattacttga caatatggac cttgaaagag aaagaggaat
     4621 cactataaag cttcaaaaca taagattaat gtataaagcg aaggatggaa atgaatatta
     4681 tttaaatctt atagatacac ctggacatgt agactttaac tatgaggtat caagaagttt
     4741 agctgcatgt gaaggagcac tattagtagt tgatgcagct caaggtgttg aggcacaaac
     4801 tttagccaat gtttatcttg ctatagacca agatttagaa atattaccaa taataaataa
     4861 aatagattta ccaagtgcaa gaccagagga agtaaaaaat gaaatagaag atttaatagg
     4921 gcttgattca agtgaagctc ctcttatttc agctaaaact ggtttaaaca tagaagatgt
     4981 tttagaagat attgtaaaaa atgttcctcc acctaaagga gataatgaag ctccattaaa
     5041 ggcattaata ttcgattctt actatgatgc ttacaaaggt gttgtagctt atgttagagt
     5101 atttgaagga actgttaaaa agggtatgac aataaaaatg atgaatacca ataaaaagtt
     5161 tgaggtaact gaagttggtg ttatggctcc aggtcaaact gaacttagtg aattatcagc
     5221 aggtgatgtt ggttatattg ctgctagtat aaaagatata agaagttgtc gtgttggtga
     5281 tacaataaca gattcaaata atcctacaga agaaccttta ccaggataca aaaaagctac
     5341 tccaatggta tattgtggta tatatccagg agaaggagaa aagtatgaga atgttagaga
     5401 tgctcttgaa aagcttcaag taaatgatgc tgcacttgaa tatgaagctg agacttcagc
     5461 agcactagga tttggtttta gatgtggttt cttaggtctt ttacatatgg agataatgca
     5521 agaaagactt gagagagaat ttaaccttga tataattact acagctccat ctgttatata
     5581 tagagttaca aaaatggatg gagaggttgt aatgatacaa aatcctgcaa acttaccaga
     5641 accatctgag ataaagatga tagaagagcc tatagtaaaa ggagatataa tagttcctaa
     5701 agactatgtg ggtgttgtta tggagctttg ccaggaaaga cgtggaaata tgttaaatat
     5761 ggaatatata gatgatagaa gagtaatgct tcactatgac cttcctctca atgaagttgt
     5821 ttacgatttc tttgatgctt taaaatcaag aactagaggt tatggttctc ttgattatga
     5881 ggttaaaggt tatgtagcat ctacacttgt aaaacttgat atattaatca ataaagagca
     5941 agtggatgca cttagtttta ttgtccatga gacacgtgct ttccctagag gaaaggcaat
     6001 gtgtgagaaa ttaaaaggag aaattccaag acatcagttt gctataccaa tacaagctgc
     6061 tgttggtaat aaggttattg caagagaaac aataagcgct ctaagaaaag atgttcttgc
     6121 taaatgttat ggtggagata tatctcgtaa gaagaaactt cttgaaaaac aaaaagaagg
     6181 taagaagcgt atgagacaaa ttggttctgt tgaagttcct caaaaagcat ttatgtctgt
     6241 gcttaaatta gatgagtaaa tccttataaa tttagataaa gatatttaaa agctagagtg
     6301 attttagaat tatttctaaa ttagtctagc ttttttatgg tatattttaa gtaaactcat
     6361 agagaatata agtaagaata ttaaagaaga ataaaaaggt gatatttatg attaatatag
     6421 aaattcctaa aaaagtagac tatataatta aagaattaga aaagaatggt tatgaagctt
     6481 atatagttgg aggatgtgtt agagattgtt tattagaaag agttccaaac gattgggata
     6541 taacaactag tgctagacca gaagtggtag tagaattatt tgaaaaaaca attccaactg
     6601 gtatacaaca tgggactgta acagttatga ttgaacatga accatttgaa gtgacgactt
     6661 atagaattga tggaaattat agtgatggta gacatccaga ttcaatagaa tttacaaata
     6721 acatagttaa ggacttgtca agaagagatt ttactatcaa ttcaattgca tacaattcaa
     6781 agacaggatt agtcgaccca tttaatggct atgaagatat acaaaataag tatatacgtt
     6841 gtgttggaaa tccagtagat agatttgaag aagatgcact tagaatgctt agagcagtta
     6901 gattttcagc acaactcaat tttaaaatag ctgaaggtac taagcaaagt attcataaaa
     6961 aagcagattt gataaaaaat gtttctattg aaagaatcca gacagagttt aataaaatat
     7021 tagtatctga ttcatctaaa ttaaacttat taaaatcaac aggtttgttg aaatttataa
     7081 ttcctgaaat atgtgaatta gaagatgtta ctcaacacaa tccatatcac atctatgatg
     7141 tacaaaaaca tactttaata gctacagaag taattgaaga tgaactgtat ttaaaactta
     7201 caatgttgtt tcatgattta ggtaaaaaag taactaaaac aactgataaa aatggtgttg
     7261 accattttta tactcatagt agagaatctg taaagattgc aaagaagatt ttgaaaagac
     7321 ttaagtatga taattatact attaataaag ttttaatatt aatacaatat catgattata
     7381 gaatagaacc taaaaggaag ataattaaaa agttattaaa taaacttgaa gatgttgaat
     7441 tatttgaaga tttaataaaa gttaattggg ctgatacact tgcaaaaaat ccaaagtatg
     7501 caaaacaaaa gatattaaat cttattgagt gtgaaaaaga atttaaacat ataataaatc
     7561 aaaaggagtg ctttaatctt aaagatttag ctattaatgg aaaagatttg atatctatag
     7621 gtataaaacc aggtaaagat ataggatata ttttaaataa gatgcttgaa atagtcataa
     7681 ataatccaga gttaaatgaa aaagaaatac taaaagaaaa agcattaaat atatatacat
     7741 tttagattca aatataaaaa atttaattac tttaaaaaac atagaataaa atacttattt
     7801 agataaaata aataattttt agagaatatt ttagaagtta cgattgtatg atttttaaat
     7861 gcataggcat ttactctagg actatagact aatataaaaa aaaactttgg gaatactaaa
     7921 accataaatt tataaaatta atattttata tactgtaaaa cagtgaggag ggacagtatg
     7981 catactcaac tacaaaagac tattggtctt tcagcagctc tttctactgt tgtaggtatg
     8041 gttataggtt cgggagtttt ttttaagcca caagcaatat acactacaac taatggagct
     8101 ccaggtctag gtattatagc gtggctttta ggtggtttta taacaataac tgctgggctt
     8161 acagctacag aaatttcagc tgctatacca aaaactggtg gtatgatgat ttatatcgaa
     8221 gaaatatatg gagaaaagtt aggtttttta actggatgga tgcaaactgt attatttttc
     8281 ccaggtacat cagcggcatt aggagtaata tttgctcaac aagcatctga acttttagga
     8341 atgagcccaa ataatatggc aaatgtactt cctatagcaa ttggagttat actattttta
     8401 gcgttattaa atataatagg ttcatcctta ggaggaaaag ttcagacagt agccactatt
     8461 ggaaagatga tacctttaat tttaataata gtctttggat ttattaaagg tcaaagtagt
     8521 gaagtattaa atccatttgt aggagatggt gtaaatgcat caaatgcact tggacaagta
     8581 ttaatagcta ctttgtttgc atatgatggt tggataaatg ttggagctat atcaggagaa
     8641 atgaagtctc cagagaaaga tttaccaaga gcaatagttg gtggtttatc attagttatg
     8701 gcagtttata ttataataaa tatagcttat ctttgggtag taccagcaag tgaattagca
     8761 acagttactt ccccagctac attagtagca acaagattgt ttggaaatat aggtggaaaa
     8821 gtaataactg tgggtatatt aatatcagta tttgggacat tgaatggata tttattaaca
     8881 ggttcaagga taccttatac attagctgaa atgggaactt taccagcatc taaaactctt
     8941 ttaaaagtaa attctggagg ttctcctgta aattcaatac tacttataac tgtgttagcc
     9001 tgtgtatatg cattatctgg tcaatttaat cttttgacag acttaacaat attttcaata
     9061 tgggtatttt atgtattgac ttttataggt gttatgaggc ttagaagaca aaagccagat
     9121 ttacacagac catataaagt ccctttatat ccaataatac caattatagc tatattaggt
     9181 ggactgtttg taataataaa tcaaatattg acttcaacag tcatatcttt aggcggaata
     9241 tttataacac tattaggttt accagtttat tattatatga aaaaaagata actacttaat
     9301 ttaagttaga taaatttggg taaaggtgaa aattaacttt tatccaaatt ttttatactg
     9361 ttatacaata taagtattat atattgcttg atgtgctatt ttgtttgatt tataatttag
     9421 taattgatgt tttaagataa tttaagaaat gaggagtgaa agatgctagg actttatgta
     9481 catattccat tttgtgtgaa aaaatgtaag tattgtgatt ttaattctta taaaatggac
     9541 atagattcaa aaaaaagata tatagaagac ttaaaaatag aaatggaatt atatagtaat
     9601 aaattatata aagataataa atataaaaat aaagaatgct gtagtctaaa taaaaatgac
     9661 aaaataacga gtatatttgt agggggaggt acacctagta tactaacctc tgatgaaata
     9721 agagaagtat ttataagtat aaaagaaatg tttgatatag atgagaatgc agaaataact
     9781 atagagtgta atcctgggac attaacatta gaaaaattaa aaactatgaa agagattggc
     9841 ataaatagac ttagtatagg gctacaagct attcaagaaa agcatttgaa ctttatagga
     9901 agaatacata cttatgaaga atttgagaaa aactataaag atgcattaag tgtaggtttt
     9961 aaaaatatta atatagattt aatgtacagt cttccaaatc aaactctatg tgattggaag
    10021 gaaactcttg aaaaagttgt acacttaaat ccaacacata tatcagctta ttcccttata
    10081 ctagaagaag gaactgaact atacaatatg tatgaaagta ataaatttga acttatagat
    10141 gaaaatgtag atatagaaat gtatgagtat acaataaact atttaaagtc aaaaggatat
    10201 aatcaatatg aaatatcaaa ctactcaaaa gaaggctata attgtgagca taatatttta
    10261 tattgggaat gtgaacatta tatagggatt ggagcaggag cttctggcta tattaatgag
    10321 aacagataca ataatgtaga atctttagaa gattatcatt taagtttggt aaaaagagaa
    10381 aaaccaatac aagaaaatga aattctttct gagaaagata tgatagaaga aaagatattt
    10441 atggggctta gaatgaataa aggcataaaa tttgaagatt ttaaaaaaaa atttggaata
    10501 gattttagag agaagtataa taagcaaata gaaatgctat tagctagaaa gcttataaat
    10561 cagagctttg aaggtataca attaactcaa aaaggaagag aaatatctaa tagtgtattt
    10621 atagaattta tggaataatt tttaaggtgt tgacaaaata aatttataat ggtatataat
    10681 atatataatc tttttagcac tcgaacttag tgagtgctaa cagctaggag gtataataat
    10741 atggaattaa atgaaagaaa attaaatatc ctcaaagcta tagttaaaga ttatatagaa
    10801 acagctgaag ctataggttc tagaacaata tctaaaagac atgatttagg tgttagtgct
    10861 gctaccataa gaaatgaaat ggctgacctt gaagaattag ggtatttaat tcagcctcat
    10921 acttctgcgg gtagagttcc atctgaaaaa ggctataagt tatatgtaaa ctctcttatg
    10981 agtaaaagcg aattggatga taatgataag attcttattg aacaatgtat gaatcataat
    11041 ataaatcata taaaagaatt gattcatgaa acttctaagc tattatcgca actaacaaac
    11101 tatacaactg tagcagttac taagagttta ataaatcaaa gtgttataaa gcacatacag
    11161 ttggttgcta tgaatgacaa taatatttta cttatagttg taacagataa aggggactta
    11221 aaaaaagcta accttacaac taatgtatat ttagatcaat ctaagttaaa tttgatttct
    11281 gataatctta caaggaagct cttaggtaaa agtataacag atttggatga taatctaatt
    11341 gcttttatta aatacgaaat aagtgagtat tctgggctta tagatgagct tttaaatgct
    11401 ttgaactcaa atatgaaaga agaagatttt tctctatcat taaatggagc tactaacata
    11461 tttagttatc cagaatttaa tgatgtatta aaagcaaagt catttttaaa tatgttggaa
    11521 aaaaaggaaa caatagctga cataattaaa tcaaagggaa tacaaaaaga caatctaaat
    11581 ataataattg gtagtgataa tgattgtgaa ctggcacagg attgtagtat agttacagca
    11641 acttataatg tagatagaga cttggttggg cgaataagct ttataggccc taccagaatg
    11701 gattatgcta gaatttactc tataataaat tatatgagtt tattaattaa tagaaagtaa
    11761 agaaatttta aattgcgagg tgtaaagtct tggataaaaa aaatgagcaa caggaagtaa
    11821 gagaagaaaa tgatacttct attaaccaag aatcggaaac tcaagtagag ttagaagagg
    11881 aagttgttaa tgaagaatgt gaaacatcaa gtgaaaaaac tgatgaaaaa gaagttgatg
    11941 atgaaaatgt aacagatata aattctaagt tagcagaaaa aaaattgcag gatgagttgg
    12001 atgaattaaa tgataaatat caaagacttc aagccgaata tgcaaactac agaagaagaa
    12061 ctcaacaaga gaaagagaca ataggcgtat ttgcaaatga aaaaataata actgaattga
    12121 taccagttat agatagtatg gaaagagcct tagatgcatg tgaagataag gaagatacta
    12181 tgtataaggg tataagcctt gtacataaac aattaataga cactttagta aaatttggag
    12241 tagaagaaat agaagcagaa tctaaggaat ttgaccctaa tctacatcta gcagttatgc
    12301 aagaaagtgt agatggagtt gaagcaaatc aaatagtaat ggtacttcaa aaaggatata
    12361 agctaggaac taaagttgta agaccttcaa tggttaaagt ttcttgctaa gtataaatta
    12421 aaggttaaat tatttaacct aatataaaat attaaaataa gtaaaactta attatataat
    12481 tactcgagga ggattttagt catgggaaaa ataataggaa tagatttagg aacaacaaat
    12541 tcatgtgttg cagtgttaga aggtggagaa gcacaaataa tagcaaatag tgaaggtatg
    12601 agaactactc catcagtagt agcatttaca aaagatggtg aaagaatagt tggagaacct
    12661 gcaaaaagac aagcagttac aaatgcagat aaaacaataa cttctataaa aactcatatg
    12721 ggaactgatt ataaagtaaa tatagatgga aaatcatata ctccacaaga aatatcagca
    12781 ataatattac aaaaattaaa atcagatgca gaaagttact taggacaaac agtaacagaa
    12841 gcagttataa cagtaccagc ttactttaca gatgctcaaa gacaagcaac taaagatgct
    12901 ggtagaatag cagggcttga tgttaagaga ataataaatg agccaacagc agcagctctt
    12961 gcttatggta tggataaatt agaccaagaa aagaaaatat tagtatttga cttaggtgga
    13021 ggaacttttg acgtatctat acttgaaata ggagatggaa cttttgaagt tttagctaca
    13081 gctggtaaca atagattagg tggagatgac tttgaccaaa tagtaataga ttatttagca
    13141 gaggaattta aaaaagctga aggtgtagat ttaagaaatg ataaaatggc tcttcaaaga
    13201 ttaaaagaag cagcagaaaa ggctaagaaa gaattatcat caacaatgag ttcaaacata
    13261 aacttacctt tcataactgc tacagcagaa ggaccaaaac acttaaatat agatttatca
    13321 agagctaaat ttgaagaatt aactagaggt ttagttgaaa aaactatgga accaactaag
    13381 acagctttac aagatgcagg actttctaca ggtgatatag atgatgtatt attagtaggt
    13441 ggttctacaa gaataccagc agtacaagaa gctgttaaga agtttatagg aaaagaacct
    13501 cataaaggta taaacccaga tgagtgtgtt gcagcaggtg cttctataca agctggagtt
    13561 ttagcagggg atgttaaaga cttattatta ctagatgtta ctccattatc tcttggtata
    13621 gaaactatgg gtaatgtaat gactaagata atagaaagaa atactacaat accaactaag
    13681 aagtctcaaa tattctcaac tgctgctgat aaccaaacag ctgttgatat acatgttctt
    13741 caaggtgaaa gaagtatggc ttatgacaat acaactttag gaagattcca attaacagat
    13801 ataccaccag cacaaagagg aatacctcaa atagaagtta cttttgatat agatgcaaat
    13861 ggtatagtta atgtttcagc taaagattta ggtacaggaa aagagcaaaa aataactata
    13921 acatcaaaca ctaatttatc agaagcagaa atagagcaaa agataaaaga agcagaaatg
    13981 aatgcagaag ctgacaaaca aaagaaagaa aaaatagaag cattcaatca agctgaatct
    14041 actatatatc aaactgaaaa aactttaaat gaacttggag ataaaataag ttcaggtgaa
    14101 aaagaggata tagaaaaagc tatagcagac ttaaaagctg taaaagataa tcaagatgct
    14161 acagcagaag aacttaagaa agctacagat gaagtaatga ctaagttcca aaaagtatct
    14221 caagaaatgt accaaaaagc agctcaagaa caacaagcgg cacaaggtgc agaacaagca
    14281 caagacaatg gacctaaaga tgacaatgta gtagatgctg actttaaaga agtagatgaa
    14341 gataaataag ctatataata tgtaacacta aatgataatt ggaacatata tgatttgtga
    14401 aaatcatata gatgttaaag atatatggac aatatacatc ataaagaata taatatatca
    14461 tagattatat aacttaaagt agaatataag aattaatgat agcttgtagt ttattattct
    14521 acaagctatc gttttgtaaa atgaaggtgg tgtcaatttt tgagtacaaa aagggattat
    14581 tatgaagtct taggtatcag taaaggtgca gaagctcaag agataaaaaa agcttataga
    14641 aaattagcaa tgaagtacca tccagataga aatcctggag ataaagaggc ggaagaaaaa
    14701 tttaaagaga taaatgaagc ttatgaagtt ctatctgatg atacaaagag aaaaacttat
    14761 gaccagtttg gacatgatgg gctaaatggt caaggcggtt ttggtggtca aggaggattt
    14821 ggcggtcaag gctttggtgg ttttgaagat atgttcggag atatatttgg tgacatgttt
    14881 ggaggaagct tcggaggtgg aagagcaaga agaagagggc ctcaacgtgg tgcagatata
    14941 agacagagtg ttactatatc ttttgaagaa gctgcatttg gtaaaaagat gtctataaaa
    15001 gtaaatagaa gtgaagaatg tgaagaatgt aatggaactg gagcaaaacc aggaacatca
    15061 aagaaaactt gttcaacttg taatggaact ggtcaagtta gaactgttca aagaactcca
    15121 tttggtaata tagcaagttc aagaccatgt agtgcttgta atggaactgg agaagtgatt
    15181 gaatctccat gtagtaaatg tcatggaact ggaaatacaa gaaaagttaa gactatagaa
    15241 gtagatatac ctgctggtat agatgatggt caaatgataa aactttctgg tcaaggtgaa
    15301 gtaggagaaa aaggtgctcc aagaggagac ttatatattg tagttaatgt taagtcacat
    15361 ccactattta ctagagatgg aaatgatatt tattttgaaa tgccaattac atttgttcaa
    15421 gcaactttag gtgatgagat agaagtacct actttggatg gaaaagttaa atatagtgtg
    15481 ccagagggga cacaaactgg aactgtattt agacttaaag agaaaggaat ccctagaatt
    15541 agaggtaatt caagaggaga ccaatatgtt aaggttgtag ttgaaattcc taaaaaatta
    15601 aacgataagc aaaaagaact attgagagaa tttgcgaagg agtgtgggtc gaacgtccac
    15661 gaaaagaaga aaaccttcgg acaaaagata gaagatatgt ttaagaaaaa gtagttaaag
    15721 tagccaatat ataatcatat ttttaaaatc ttagttattt ataagattgt caaaggagat
    15781 attatgattt ttatattggc tatttttgta taaaaaataa atgtgcataa aataaaactg
    15841 aatataaata attttctgaa aaatacttgt cctaaaatat ataatatgct ataatataat
    15901 caaataaaag aaaggggtga ttccagtggg aacggatgtg gcactgccaa atgatgaaga
    15961 aaactttatt gtaatataca aaataaaatt agatactttt agtgctagat taagtactgg
    16021 aaaaactcat gagctgggat aattattgta attacctaga ctcaaacttt tagtagtaaa
    16081 atagtaatat tatatagaca aggtatctaa tttttaaatc ttttgtatga attaaaattt
    16141 ttataaaggg tatatctgat tagaatgtaa ttagggaaac ctagttgtga attactttta
    16201 gtatatacat gtgtaaagaa atatgtatat ggtattggtt attgtgctag atgtgtatgt
    16261 aaaggaatat gtgtctagtt gggtatatgt aaagaaatat acttataatc tatgaaaaat
    16321 ttttagtata aattgctaaa acattgagat tgttttttat agaaagttag tttataccaa
    16381 ctaaaaatgt gcttaagatt tgtttgatgt ttaattataa ataaggttgt agataaaaaa
    16441 ataagtaaag acaaaggttt aaaatacttg ctttaaatat agtttgtttt gaacacttgc
    16501 caaattaaaa taattaataa aatagacatc ttctcatagt aaaatatact gtgagaggat
    16561 gtttttgctt tgttcataaa cttttatgaa aaatttgcat agtgtcacat tttaatatac
    16621 tatatatata ataaaatata gataaaatta aatgattcaa aagagaaagg tgaaaagaga
    16681 tgtattatat aggtgtagac ataggaggaa caggtataca agcaggagta gttgataact
    16741 atgggaaaat aatttttagg tcagagtgta aaacagtaat tgaaaaaggc tttgaaggaa
    16801 tacttaatga cataaaaata atgatttata agttgttgga agataataaa cttactatga
    16861 gtgacataaa atctattgga tttggtgttc caggttttat aaataaagaa ggattagtaa
    16921 cttgtgtaaa tttaaaatgg aataaaaaag cttttaataa agagttaaaa agaagatttc
    16981 cagatgttga aattcatgga gaaaatgatg caacagtagc agcacttgga gaagcaaagt
    17041 ttggaagtat gaaaggtgct aatgttggag tattatatac tttaggaact ggagtaggtg
    17101 gaggaatagt aattaatcaa aaagtctttt ctggagcaca tggattgggt tcagaaatag
    17161 gtcatcaaat tataggagaa aactatttta attgtaattg tggaaataat ggttgtgtag
    17221 aaactttttg ttcagcaact gcaattataa agtattctca aaaactgata gaagaaggag
    17281 aaaagagtag gattttagat ttggcagagg gaaatttaga aaatgtaaat gcaaagatgg
    17341 tgtttgatgc atatagagaa aatgatttag tggcaattaa agtaataaat agatttaaag
    17401 agtatttagc aaaaactttt gcaaacacaa taaattcttt agacccagaa attatatcta
    17461 taggaggagg catttctaaa tctagcgata taatacttga tggaatagaa gatttggtaa
    17521 gaaaatttgt attatacaaa acagaagata tagctacaat tacatgtgca actttaggtt
    17581 cagacgcagg aattatagga gcagcttttt tataatgtaa atgaactgct tattggaggc
    17641 gtgatgatat atgggtaaaa caaatgctag aagaacgctt tttttgatag caataggttc
    17701 aggaactatg ctgaatcctc ttaattcttc tatgatttca cttgctttac atagtataca
    17761 aaatgatttt catattagtt ttacaactgt atcttggatt gtttcagcat tttacctagc
    17821 tagtgctatt gcacaaccag tttcaggtaa gataggagat ttaataggta gaaaggtctt
    17881 atttttgagt ggtttgatgc ttgtaatttt atcagcatcc atagtaccac ttgttcaatc
    17941 attctttata ttgattttta taagagtaat tcaagcaatt ggaacaagta ccctatatcc
    18001 atctggagtt gctcttatac aaaataatat aaaagaaaga caatcttctg cattagcagt
    18061 tctaaccatt tttgcttcaa ctacagcagg attaggtccg acacttggag gattattgtt
    18121 ggacttagga ggatggcatg ctatatttct tgtaaatata cctgttgttt tggtttctat
    18181 atgcttaggg tattttttat tcccaaaaga agtaaaagaa aagaaatcta taaaagaaac
    18241 tttaaaaaat gttttgtcaa gattagatat tattggcata cttctatttt caattgcaat
    18301 gatttttata ttactatttt tgctatctat gaaggagtct tttaacttgg aacaacttgt
    18361 atttggtata atactgatgt gtctttttat ttggcatgaa ttgagaacaa aattgccatt
    18421 tatagatatt aagctttttg taagtaatcc taaattgtca aaagtttact tgcaatttat
    18481 tatattaaat ttctttaatt atattttgtt ttttggtctt cctagttatt ttcaagatgc
    18541 cttacattat agtgcaaagt cgactggatt atttatgcta tttatgtctg gtataggtat
    18601 atttatatct ccattaactg gaaaatggat agataaatct ggaactagat ttcctcttgt
    18661 tacatcatca atctttatgt ttattagtgc agtacttctt tcagtagttt ttatccatat
    18721 acctgtaata ggaaagggta ttatattatc attagcaggt attagttatg gagttggtaa
    18781 tgtagccctg caatcctcta tgtttgaaga aagtcctcgt gacagtatag ggacagcttc
    18841 tggtctgttt caaactagtc gttatttagg ttctatttta gcaactgttt tacttggaat
    18901 ggtatttgaa gtaaatataa catcagaaca atttcagata ttaggttatg ttatggtggt
    18961 cttaggtaca ataagttttt tattaaattt tatattaaag aaagaattaa gaaaagtaga
    19021 atagtggatt tatggattta ataagtctaa taactgataa gtttaaaaat attgtttgta
    19081 aaacataact ggtttgatat ttattaattc taagttttga agcatagagt actcgataag
    19141 tatacaatat tttgaattaa aattaagcag aaatttgaac tagtctttca aatctctgct
    19201 taatttagta gttaaatttt tttatagaga aaataagatt tgacacttag ataaaaaatc
    19261 attgcatttt caaaattctt taaatcaaac ccaagtatct cattaatctt ttccaatcta
    19321 tatactagcg tgtttctatg taaaaacata ctagtagatg tcttagaaat atttaaatca
    19381 ttatttataa attttattcc tgtatataga agttcttcgt caatagattt aatcttatta
    19441 aaatctatta atttattgag tatattatcc tcattaatta aatctaaagt tttatatcta
    19501 ataaagtcaa acaaatttaa aaacttattt tcagcgttgt cttttataat atgaggtagt
    19561 aaatcagaac ataactgtaa tctaaaatca aaatcagctt tactatctaa ttcaccaaga
    19621 taaaaaatat aagtactttg ataagtttca gtttcaatta tattttgtgt agttacat
//
LOCUS       DAYAOY010000078        19342 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYAOY010000078 DAYAOY010000000
VERSION     DAYAOY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.078"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            332..913
                     /locus_tag="ACGI6B_002817"
     CDS             332..913
                     /locus_tag="ACGI6B_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9752251.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="ACGI6B_002818"
     CDS             952..1530
                     /locus_tag="ACGI6B_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9752252.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="ACGI6B_002819"
     CDS             1616..2245
                     /locus_tag="ACGI6B_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9752253.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="ACGI6B_002820"
     CDS             2235..4994
                     /locus_tag="ACGI6B_002820"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9752254.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="ACGI6B_002821"
     CDS             5081..6643
                     /locus_tag="ACGI6B_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HFG9752255.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="ACGI6B_002822"
     CDS             6644..7780
                     /locus_tag="ACGI6B_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFG9752256.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="ACGI6B_002823"
     CDS             7844..8920
                     /locus_tag="ACGI6B_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFG9752257.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="ACGI6B_002824"
     CDS             8898..10136
                     /locus_tag="ACGI6B_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFG9752258.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="ACGI6B_002825"
     CDS             10190..11278
                     /locus_tag="ACGI6B_002825"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFG9752259.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="ACGI6B_002826"
     CDS             11306..12097
                     /locus_tag="ACGI6B_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFG9752260.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="ACGI6B_002827"
     CDS             complement(12212..12766)
                     /locus_tag="ACGI6B_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9752261.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="ACGI6B_002828"
     CDS             12962..14164
                     /locus_tag="ACGI6B_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9752262.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="ACGI6B_002829"
     CDS             14221..15201
                     /locus_tag="ACGI6B_002829"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFG9752263.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="ACGI6B_002830"
     CDS             15444..16397
                     /locus_tag="ACGI6B_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFG9752264.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="ACGI6B_002831"
     CDS             16387..17343
                     /locus_tag="ACGI6B_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFG9752265.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="ACGI6B_002832"
     CDS             17340..18095
                     /locus_tag="ACGI6B_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752266.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="ACGI6B_002833"
     CDS             18187..19152
                     /locus_tag="ACGI6B_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9752267.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7650 a         1970 c         3457 g         6265 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataataa gataaatcta ttcaaaatct aataaaatat gaagttatac
    19321 tagacaaaaa gaataactct ta
//
LOCUS       DAYAOY010000079        19304 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYAOY010000079 DAYAOY010000000
VERSION     DAYAOY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.079"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(400..936)
                     /locus_tag="ACGI6B_002834"
     CDS             complement(400..936)
                     /locus_tag="ACGI6B_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFG9752268.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(954..2267)
                     /locus_tag="ACGI6B_002835"
     CDS             complement(954..2267)
                     /locus_tag="ACGI6B_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFG9752269.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2622..3062
                     /locus_tag="ACGI6B_002836"
     CDS             2622..3062
                     /locus_tag="ACGI6B_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFG9752270.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3246..3434)
                     /locus_tag="ACGI6B_002837"
     CDS             complement(3246..3434)
                     /locus_tag="ACGI6B_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9752271.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4456..4851
                     /locus_tag="ACGI6B_002838"
     CDS             4456..4851
                     /locus_tag="ACGI6B_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HFG9752272.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4894..6993
                     /locus_tag="ACGI6B_002839"
     CDS             4894..6993
                     /locus_tag="ACGI6B_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9752273.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(7218..12428)
                     /locus_tag="ACGI6B_002840"
     CDS             complement(7218..12428)
                     /locus_tag="ACGI6B_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFG9752274.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12443..14938)
                     /locus_tag="ACGI6B_002841"
     CDS             complement(12443..14938)
                     /locus_tag="ACGI6B_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9752275.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15599..15886)
                     /locus_tag="ACGI6B_002842"
     CDS             complement(15599..15886)
                     /locus_tag="ACGI6B_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752276.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15906..16181)
                     /locus_tag="ACGI6B_002843"
     CDS             complement(15906..16181)
                     /locus_tag="ACGI6B_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752277.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16816..16995)
                     /locus_tag="ACGI6B_002844"
     CDS             complement(16816..16995)
                     /locus_tag="ACGI6B_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HFG9752278.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17280..18755)
                     /locus_tag="ACGI6B_002845"
     CDS             complement(17280..18755)
                     /locus_tag="ACGI6B_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9752279.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         6433 a         3347 c         2089 g         7435 t
ORIGIN      
        1 ataagtatct ttttttctta ttttacctct aaaatttttc taattaatct ttcataaata
       61 tctattttaa ataaataata tagagtttat acaatttttt atagcttaat tactatttac
      121 tatatattat tcatttttaa ataaaatgtg attatttttt attaaaaacg atataaatat
      181 acaatttaat actatttttt tataaataaa cttgtattaa aatacattta gttatataat
      241 tttatttttt ctaagagtat aattataaaa ataaaaaaag aataggataa ttttcctact
      301 cttttttctg tacttacata taatattaaa actctttatt ttttaatata tattttaaat
      361 tttctttttt aataaatctt tttcttttaa taaatctatt taattttctt cttctatatc
      421 agagccaaag ggaatccatg cattaccaaa ctctttatct ttaaataaat aagctaatcc
      481 agttatttgc ttcaatctaa gaatttcatc aacttccata cccaggtgct ttgatatcca
      541 ttcatcagat tttccaattt cacaaagttc agttataatt ttactcatta aatccacatt
      601 atgagaacct cttgcacgat tgtgtcgaat agtacttgcc attctttcat caagagattt
      661 atcaattact gaaacaggaa gaagtccatt ttctctatca tatatatcct cattttcaag
      721 cattactcta tatctatgaa aaccatctac aataatatac ttatcctttt cctcattata
      781 ataacagaca attggcattg tatacccatc ttcttttata cttttataca acaactgcat
      841 ttctggtggg gctactgcat ttggattata gtcgttagat tctatctttt cgattggcac
      901 tgcgatgatt ccatatacag gacttttata ttccatagca tcttaactcc tatctaatga
      961 attttttcat atttcttctt aatatattcc gttctaattc tctgttcttt tgtaggacca
     1021 aatcccataa atctacacat atggtcattt tttaaaatac aataacacat tcttttccaa
     1081 cttggtatat ctttggtaaa tttaatatca tccgtatcat caggtatatc atccaaaaat
     1141 atgactctac tctttttatc ttttgtatga tttgaaactc catttaattc tattttataa
     1201 ccttttctca acaattcacg tataactatt tctgacaaac caccacctgt attctgccaa
     1261 aactcaatag atgttgaaaa ttttttaata tagctattcc ttatattttc aggtaaggta
     1321 tcaagcaaaa aatatgtgaa acttttccat gtgtgtccat atggcaattt aacactacga
     1381 tatcccatag cttttgtttt gccataaata gaagtaaaat taactccttg tactctagca
     1441 accaatttca cccatacatc tgggtccaat attctgtata aatttaaact ctcttttgct
     1501 gaatcattaa aaggtgatgc cactctcatt tttcttggag ctactccagc tttatagaat
     1561 aagtcataaa ttttattata gtcatagtca aatttatagt gtgctaacca tatatcttct
     1621 atttcccaat catatatagg agatgccgtc cagacatttt taaattcctt agttatccag
     1681 cactttcctt tatatccatg ctgtttattc acaatagaac aataacgttg tagagattct
     1741 gctgctcgtg ttcctaataa gcaaacaact tttgcacctt catgtatatc tctaatccat
     1801 ctaccaaatt gttttgccaa actttcttgt ctcattttat atttataata agtaaaagga
     1861 ttattttttt gatttattat gtattctttg actggcattt cccttaccca taactcttct
     1921 ttctcatcat cccatggata ccaatacatt tcataatttc ctacagcagt tcttgtagcc
     1981 attggtaaac acacccaata tggttcaata tcttcaatat tatcttcaaa tactttttca
     2041 acatattcag tagtcaaact atattgagcc tcaaaatctt gatgaaatac tcctattttg
     2101 gaacttatat tattctttct cttataatcc aaaactaaat ttagcagtaa accactatct
     2161 tttcctccgc taaaagatac atagatataa tcaaattcat taaaaagata agaaagtctt
     2221 tcttgaaaag cttcaaatac atcggattct aaatatctct tatccatttg aattatcccc
     2281 tttcaattag aagtttataa aaccttacaa tgcaatatta ttatgctttt ttagcagtaa
     2341 tcagcataaa ttatacatta tttaaataca caaatccaac taactacata taattataat
     2401 gtaattatac ttttgtatta taaactatat tttatcatta ttttacattt ttttccaata
     2461 tatataaata tcatattttt ctattagcta aaactaatta ctttatattt ctacgtatta
     2521 gtataataat acataaaaac ttgaataatg cataaaaact tgacatactg ttgtcataat
     2581 tactttgcta taattaaaag taaaaatgga ggtaaattct aatgtatgaa cgtatgcttg
     2641 ataaaagcaa taaaccctgt ttgtcccaaa taatagaata tataggtaaa gatggatatg
     2701 cacgattgtg taagttggaa acttacttga aaactcaata taatttatca agagaacttc
     2761 gatttccttt tggaaacaat tatggttggg gttacaaata tagccataaa tcttttcatt
     2821 tattttatgc tttttttgaa aaaggtgcat ttactgttct gattcagata ggtgacaagc
     2881 aagttccagc agtagaaaaa acgttggtag ctttgacacc aaaagctaaa gaactctggg
     2941 tcactcgtta tccttgtggc aataatggag gctggattaa gtatcgtatt ttagaagatg
     3001 cagacttact tgatattatt caatatataa gagcaaaaaa atctccacct aaacaaaatt
     3061 aacttttatt ttttgtgatt taaatatagt gtatataaat tccattcttt ccatattaaa
     3121 aaataatata aaaaatatct attagtataa cttatttagt atgtcttatt ctacttttta
     3181 cagataatta aaaagtttta tcataaataa atcataacta gacatcctag taaatcaaaa
     3241 ttaatttatt cttttttatt cgaatttttg ataatagtcc ctatacatat gccaattatc
     3301 attccaaaaa ttagaccata acacattgta atacctacat ttcctttaga aatacctata
     3361 gcacaaccta tagccattcc aagtatcatg cctataaaca tacatataat aactaaattg
     3421 ttgtttttat tcatagtttt tctccattca atatatttta aattgcctag ctcgccttct
     3481 tagcttaaaa caaagaaata taaattaact ttctttcatt ctaatatctg cctgtataaa
     3541 tgtcaaacaa cacatttctt gtcataacct cctctttttg aagtaagtac aatctcccag
     3601 acgcaataat atcaatttcc taaaaccatt taagtaactt ttatatttac ataatattta
     3661 caatcataaa ctatattgtt attcatttat acatcataaa cactccctcc taatcattat
     3721 tatacttaaa ataatgttca tttataataa tatatctaac gcactttaat tgaaaaaact
     3781 aaaactttgc ctttcattcc catatataaa taagtatact ttttgatgct ctattacaaa
     3841 tcaactaaat taattgatat aaattatcat tttctgttga aaataccatt cgcacatgct
     3901 atacttaatt gcataaatgg taataaatgt catatgattt tacaaatcta ttaataacaa
     3961 ttattacttt tactgtattt taaagttttt ggactaaata tcaagaactt tctaaaatac
     4021 aaagtacaaa aaatttatat aagttataaa tttatatgaa ctagataagc tcaaacaata
     4081 tgttatgtat aaactgtgta gtattcggtt ataaatttat ataaattata taagctagat
     4141 aaagtattac ctcaactata taagctagat atataaattg cttatataag ctagataaga
     4201 attatttcaa ctatataaac tacatatata aattacatac ataagctaga tatataaact
     4261 gcatatataa gctatataag aattatttca accatataaa ctacacatgt aaaattcata
     4321 taaatttact aatataaatc aaataaaatt actaaactct ctaaactaat tttgtaaaac
     4381 atactctatt tttaacattt gttaccttat acataagtac aaacaaaatc taactaaatg
     4441 aaaggataat gtaaaatgaa tattttaaaa atgaaaaaaa cagggatatt tttaggagga
     4501 gtactttttg gtactgtagg tgttaagata ctttcaagtg attgtgttaa aaaaacttgc
     4561 actcgtgcaa cagcaggagc attgaaagtt aaagattctg ttcttgaaac aactacgaaa
     4621 attcaagaaa atgtagaaga tattttagct gaagcaaaag aaatcaacga gcaagaagac
     4681 ttatctacat ctaaggtaaa cgttgtacaa gatactctta aatgcgaaca agaagttctt
     4741 aaaactgaaa ctataaaaac tgtagaagat actaattcta atagcattaa atgtgaagaa
     4801 atcaaagata acgtaattga agaaaaaaat agtacttgta caacagaata aatttaatga
     4861 ttattatgat taataatatc aggaggaagt aaattgaatt tttatataaa acacgaaata
     4921 agaggtcgta tacgctttga cttacaactt ggcaaattaa acaacaagca gtccgatact
     4981 ctcttatatt atttaacttc tataaatggt attaccaaag caaaagttta cgatagaact
     5041 ggtgatgcag tcatttatta cactggtgaa agagaataca taataagaga aatcactaaa
     5101 ttcagtttta acaatagcaa aatagaaaac ctagttcctg aacataccag cagggaatta
     5161 aacaattatt atagagacaa attagcaaat aaactgctag tcaaaagtat ctccaaaata
     5221 ttttttccaa agtccttaag atttttttta attggacttc attctataaa atattttaaa
     5281 aaaggtctct cttccctgct aaataaaaaa atagaagttt ctgttttaga tgccactgct
     5341 ataggtattt ctatttttag aaaagatatc aatacggctg gttcagttat gtttcttctt
     5401 ggtatcggtg aactattaga agaatggact agaaaaaaat ctatagacga cttggctcaa
     5461 agtatgtcac taaacattga aaaagtatgg ttaagaaaag gtgacactga aatcttaatt
     5521 ccaatttcag agataaagga aggagattta gtaagtgtaa ctatgggaaa tatgattcca
     5581 ttggatggag ttatagtatc aggagaaaca atggtcaatc aggcatcact tacaggtgaa
     5641 tcccttgccg ttaacaaaaa agaaggctct tacatatatg ctggaactgt aatagagcaa
     5701 ggcaatattg ttatgtgcgt caaggaaaaa gctggtacta ctagattcca gaaaatagta
     5761 actatgattg aggaatctga aaaactaaaa tcttctgtag agagtaaatt tgaacatcta
     5821 gcagatacat tagttccata cagcttttta ggaagtatat taacctatgc aattactaga
     5881 aaccctataa aatcactctc tatattaatg gtagatttct cttgtgcatt aaaactctct
     5941 atccctattt cagtactatc agccatgaga gaatgtaaca ataacaacat tactgtaaaa
     6001 ggtggaaaat tccttgaagg tgttgcatgt gcagatacta ttgtatttga taagacaggc
     6061 acattaacaa aagctcaacc aactgtttca gatataatta cattccaaaa ttataataag
     6121 gaggatatgt taagacttgc agcttgctta gaagaacact ttccacactc catagcaaat
     6181 gcagtagttt acgaagctga aaaacaaggg cttagtcaca aagaaatgca tacagaggta
     6241 gaatatatta ttgctcatgg catttcaaca aaagttgatg ataaaaaggt tgtaataggt
     6301 agcaaacatt ttgtatttga agatgagaag tgtactatcc cagacggtga ggaggataag
     6361 tacaacaatc tatcagatga gtattcacac ctatttatgg caatatcagg taaattatca
     6421 gcagttattt gcataaatga ccctttacga aaagaagcaa aatatgtaat ttcaaattta
     6481 agagaatgcg gaataaaaaa gattgtgatg atgactggag atagcgaaaa aacagctaaa
     6541 tctatagcaa gtaaagttgg tgttgatgaa tattattcag aagtcttacc agaagacaaa
     6601 gccaattttg ttaaaagtga aaaactcaaa ggtcgcaaag taattatgat tggtgatgga
     6661 atcaatgatt ctcctgctat ttcagaatca gatgtcggaa ttgcaatgag tgaaggtgct
     6721 gaaatagcaa gagagatttc agatattacc atatcagcag ataatttgaa taatttaatc
     6781 gttttaaagc agattagtaa taagcttatg aaaagagttg atttaagttc taagtttatt
     6841 attggcttta atttaggtct gattttactt ggtgtaggtg gatttgttag accaagtaca
     6901 tctgcctttt tacacaacac atctactgtc ggaataagtt taaacagtat gacaaattta
     6961 ttggaaaaca ccaatactta taactaccat taatctccat tacataaaac atatgatata
     7021 aaaaatttat aattcatctt tttaaaagaa tatgtcttaa aattagcttt tttctaatca
     7081 aaattatata aagcatactt tacaaatttt aaactcaact tatttctatt ttttacaaaa
     7141 aaggagatga ttcaaatttt cattttgaaa catccccttt tgtaaatttc tttaaaattt
     7201 atatattaat tatattttca accaataatt gtattttcaa ccaatctttc ttcatagcat
     7261 tttaaatcat tttgctttag ataattaaat tcagactcta cataaatacc acagcctacc
     7321 aattcaattg tcaaattagg tatattttcc tttagtatac tagtctgctc ataaataatt
     7381 ctttccaata aatcaacttg taatatagaa aaatgcttca taatctcccc atcactatct
     7441 atatctttct tgatttcata ccaactcttt gacaaaattc ccttcaaatc aatataccct
     7501 agactcctca atatatctat actatcgctc catgcatact tactaggttt atcaaagtat
     7561 gattctagtt tcttcttctg ctcatttgta agtgaatcat aagaaagctt cttattaaaa
     7621 tgtgccttca aagaaatctt agccatagaa tatggacttc taccagagtt taatcctgca
     7681 tttttgaact tttcaattcc tttatcttct actgaagacc atattgagtt tattgaatct
     7741 gaatcattgt atttaatatc aagaccatgt gtaatattta tatagttttg tggaaataca
     7801 tattgataat agacatcttt tttatcatca tatagagaga agtatccact atcatataaa
     7861 tgaatatgca cctttacttt taaactatcc tctaaaatat ttcgtctaag gttcgggata
     7921 tttgcataca tggttacata ttttatggca tcaaagtttt tatttaatgg aacttcaaaa
     7981 ctaatttcat ctcctgtcaa aagctttttc acttcttctt cattacaatc atatagagaa
     8041 tatgtatttt caaatcccac acgtgatttc ccatctaaac tgcctgttaa tgctaccttc
     8101 aaaatatggt tatctgtttt ttgccattgg taagcatctt ggtcaagaaa ctctctaatc
     8161 tgtctatttt gctctttcgc ctgatttata acctcgtttt catcttctgt aatccctaca
     8221 tggcatctat ttaagatatt tactatattg cttccaaatg ggaatgtcca tagttgaaaa
     8281 gctgctgcct caacctgaca caaatatcta acctgattta attcaatttg atggataagt
     8341 tttttctgat attcattcat ctgattacca ctatctgaca tttgataaat caattctccc
     8401 attgcaagat tatctttttt cttatcaatc ttatcagtaa tgataatttg agtttccatc
     8461 atatcctgta cttttttaaa tattatagaa atctcctctg agttttccat ttttaagctt
     8521 ccaaacttcg tgcttaattc agttaaaact tccttcatct ttaatttact tatctcattt
     8581 tcaaaaatat cttgattttt aactgtacta tcgaattttt ctacaaatgg tataacagat
     8641 tctttcaaag ttttatcttt aaattccacc atttgagtat tcaattcatc taatttctta
     8701 atattatttt ctttatcttt aattgcctct ttaactacct ctattttact ctcaaaacta
     8761 gattttacat taagacaatt attaacacca ctaatcgcag tatttataag agaacaagca
     8821 gaacttccaa tactcaatgc atttttaatt ttagatgtct tttgctgtct ttcaatagct
     8881 tgtttacttt tctctagtgc agctaactta tctttttgct gtttattatt gtaatttgcc
     8941 tccaacatac ctatttcttt tttctggatt tttgtgtaaa tttccttata ctcgctttca
     9001 cttaacccct cttttgctct ttgaatatcg tcagctttgt ctgcatagtt atcatcatct
     9061 atagacttaa ctataatctc cttaatttgg tcaaatttag ttcttctttc ttcatcaaca
     9121 cctgccaaat tataagtcat tttatctaaa gcattttttc tatcttcact tagctgttct
     9181 ccaagtacaa atctcatacc atcagaatcc aatttaaaat ctgaaaaatc aaccatcctg
     9241 ttagtccatt ggtattcctc ttctttagct gaaagatttt ccttcaaaga ttttgcactg
     9301 tcaaatgccc ctagacaagc ttgaccaaca ccactaaatg cagtcagtaa actcaatgcg
     9361 ctttgtattc cagaaagaat taactgtgtt ttcatttgtt cctcaagttt tgcctgtttc
     9421 ttttttagtt cctctatgtc ttcttgttca tcttctttca tctttataat ttcttgaatc
     9481 aacaggttca tttttgcatt aacagaaagc attttattct caagttcatt ttttaaaacc
     9541 tctatttgtt gatttccaga ttcaatttga gttaggatac tttccttata tgtattgaaa
     9601 tatccttctt ttaactcatc tttctttcct tgttccaatt ctcttaaggt cttatccaga
     9661 tagtctgtca aagaacgaat atctgtaaca cgatttcctc caatttcact ttcttcaatc
     9721 atctgcacat aaaatgctgt cttctgctca agtgcaaata aaatcatact ccattcgcta
     9781 aaattctgat tttttcctac aaaagattct attctatcag actgcatcaa tttatttata
     9841 aggtattcat agatttcctt tgcctcttta taaaattctt tctttatatt catgtcatcc
     9901 atagaaagta ctgctttatt tttcttctca aaactatagt acttctgaag tatttctcta
     9961 tttaattgca tctctatttt ctcaactgca taaaaaattt cataaatatg tgaaaattct
    10021 aaaatatcag cttctaaaat tcctactgca cacaacagtc taagatttct ataaccttta
    10081 attccagaaa aaccaaatct ctcgaaaaat tcatagaaga atgtatctat tgacaaagaa
    10141 tactcctgct ttaacaattc cttttctttt agtgcatctc cagagtgacc ggcttttccc
    10201 tctgctccgg ctgaacctga atcttcacgt ttgctaattg tccagtcctt atgttcttca
    10261 taaagagtat ttaatttctc tccatcatct gctgcttgct tctgatttaa agcatgatta
    10321 aacacatatc tatcaaaata atagtagttt ctatcactat tatccattgg aggagaggta
    10381 cctatatatc ttattgtttc ttcatatata cctctaattc caccaagacc tcctgcacca
    10441 ccagctcctc cagagcctcc ttttcctgct aaaccatgat ttccatcttt tttacttata
    10501 gcacttttta ataaaggagc ttttccaagg agataaacct gtccagactt gcttccaaga
    10561 cctgcatttc caccattacc tcctgtgcct ccagttccac cttgttttcc tttttctgct
    10621 tttacaacat agtattttgt acctttaaca ttagcaaatt cttttgactc ttttttagaa
    10681 cctattttag catttgcccc agcttctcca gttatccctg tatctccttt ttttccatct
    10741 tgtccatttc caccacgtcc accatctacg taaagtttaa gttctcctct tttttctatt
    10801 tctttaaaag ataatatgat atcacctgaa gtacctgcat tattgccatt tcttccattc
    10861 attccgtcgt aaatagaact ttctccaacc aatttttctg catccttacc agatacgttg
    10921 attgaataag tcccttctgg tatatgtaat ttttctgtaa gtagtacaat attcttacct
    10981 gagaagactt ctttctccat attacagtca attttcaact cacaatctcc atacatatat
    11041 atagatgaca aaatattaag ttgataacca gatattattt tagtcaaatc cgataaattc
    11101 actttaggta taggaatata tatagaaagt acattttctt tctcatatga ttttatatat
    11161 gtacaacttt gaatcttctc tatcctatac tctgcatctt caagcttttt ggcttcttct
    11221 tccctatatt cttcttctgc tatccaatat ttgatatctt ttaatgtatc ttctaaagta
    11281 ttttttagca atgattttaa caactcactt agaccaacaa tatgagtctc actacagtaa
    11341 ctatttaact ctaaaagaag acgtgtttct tctaatatat tcactttctg tacgccatca
    11401 ccatatgaaa gttcttgtac aaactccata agctctttca tattattagt cctactacat
    11461 aaatctgtat acatcttatc taattgaatc tttttatctt ttagactatt tatagaagat
    11521 agaatggaaa tagaatcttc catacctttt ttaacacttt ctatataagg aactaaaagt
    11581 ttttctaggt ctttataggt tacatcaaca gctttttgta tttctatttt acaagaagct
    11641 gtaagcatca atcccaaatc tgttttttca aatgtttcag gtagattttt catttcatcc
    11701 aacaactttt ctggctgttt atctagtgga ttacataatt taatagatac accatctgat
    11761 aatgctacat ttaataagta cactaattcc ttacaaccgt cgtaatcttt ctcttgacta
    11821 tctaagtctt cttttgcttt ttccaatata tttcttattt tttcttctgt tacattaccg
    11881 attagtgttt taggtatttt attaataaga aagaagaaat tttctttata ctttgcataa
    11941 tcaccaagca tatctttcaa atttttgcct gtttctatca ataatgtatg tctagagtcc
    12001 agtagggatt gatagtctaa gataactacc atccctttca cctttgaaga tgtggaaata
    12061 gtttccctta caagcatagc accagcaatc atatactctg ctccacggtt gtcaaaaaaa
    12121 cctgggcaat cacataatga aaaatctcta tttgcaatat ctacaacttc tggacataaa
    12181 gtcttagaat atacaccatt tccaatttcg acagctgaat cttttgcagt tatatatatt
    12241 tgtccagtct cttcatccgt tttttcttcc atcacacagc cttttaaata atttatacaa
    12301 gtactctttc caacgcctga agcacctagg aataacaaaa ttgacttttc ttttaagtca
    12361 tattttctaa gctcgtctct tccttcttcc aaaaggttta cgatttcact ataaagttta
    12421 tttgccatac atccatccct ttctatattt cggaacaata gccttgatat ttttctttaa
    12481 taagattctc ttctaccttt gaaacagctg ttgataactt agtatttgga gcattaaatc
    12541 ctccattgtc tcctctatct ccatattcag cgtattctct tgctggtcct gttttacctt
    12601 cattccagct tccaccacta aattctatat gatatttaat tatactatct aacttataat
    12661 cccaacttgt aaaatatcct gtatagtata cagtcttatc tataccactt ggagaaaatg
    12721 aaagtgtatt atatccgtga tagccaccac ttcctccata tccacctaaa cctggtctac
    12781 catttggacc tgcttctcca tctttcactg ttatatcaca atgattaatt attctatttt
    12841 tttgttcctc tgaatctgaa atgaagtaaa tattaccttt cttaccagca tttccacctc
    12901 ttccagcatt tccaccagct ccaccttttt cacctttttt gccacttgta cctttttcaa
    12961 aatacccttt aaacatctgt ccgtggaaat aacttgtata tcctgcatca gctccattac
    13021 ttccagaagc tcctgtttca ccattttctc catctattcc tttagaacca tttcctccat
    13081 ttgcaatcag tgttagacga taatttctaa tttcttcttt aatatataag taaatattac
    13141 ctgcatctct accatcttca ccatttttcc catacataga attatctaca agttctccgc
    13201 tctttccact tacattaatt tttacattac tacctacatc aagattgtta gaagcaagaa
    13261 taatattttt ctctgatgca aagttcattc caaggttatc atccatataa atagtatctt
    13321 tagcaaagaa cataaaagta gaaacattgt catctacttt tctatttttg aaatattcca
    13381 atacactgct aattactaag tttggaatta atgctcttat ttcaatacct ttcttatcat
    13441 taatattaat tgtttttact ataaatggat ttaaaatgta tttaccaata aattctctaa
    13501 ttatctcatt accttcttct ccatatgcac ttaatcttgt tatactgtct tctaactctt
    13561 cattttgtgt aactgtaaca tttgtcaaaa ttgaattaaa atcacttaca ctatatacat
    13621 aatccttttt atttgcaact tccattaatt tttctctatt tggtaatgta aatacctgtt
    13681 tttgcacttc ataagaaact aaaacttttt ctaaattctt tcttattata atgaagtttt
    13741 gttccattat cttcttactt gccttttcta taataaattc catatgtaca gaaggtaata
    13801 aaatttcccc agttccatgt tctgtatatt tacttaaagc atcaattata tctaagtttc
    13861 cttttatctt attttgtaat ttcatgcaac tatcttgtaa gaataatcca ctttgtttgg
    13921 ctaatacgtt gataaaactc tctatagtaa gacttgttgt actttccaac cagtttttaa
    13981 atacttttac aacctcatca tattttgcta tttcttgaat cattgttata cttccaataa
    14041 tattgataaa atcttctgta tacgccttaa cataatcttc taggctagat acgatattgt
    14101 tttgaatttc ttttatcaac gtatctaaaa ctaatcttgc atctgcacta attggatatt
    14161 ggaatgctga tgtcttatct ttaacttcat ctaattctag tacttgcttt aataagcctt
    14221 ccctctcatc tgatgcaagt ggcttacaaa gatgaatatt ttttccatta tcttttagca
    14281 attcttcaaa taaccaagct ttttcctcta tattactgtc taaattttcg cataattgtt
    14341 catattttct ttttataaca ttatatattt gtttttctgt tcttcctaag aaactttttg
    14401 aaatgaaaaa agttatacta cccttaaaat tttctttatg aagacacata tttaactgtt
    14461 ggaacaggtc tagtactttt gtaagtctag aatctaatat atctgcttct ggtatcaata
    14521 caattactgc acatatagat gcagaatttt taaatacttc acctaatgaa actgctgcac
    14581 taatatcatg ggctactccc ctattgtcgc taaaacctgc agtatcacat aattttgcat
    14641 ccattccttc tattggtttt ctcatattta caatgttcgg gtataaagtt tgagaaacag
    14701 ctcctgaacc aatctctgcc acagggtcta agcaatcaat atatgatata ccagtatctt
    14761 catcttctac ttccatcatt acacatcctg caagatagtt aattgcagta cttttgccac
    14821 atcctgtccc accaagaatt agaattaaat tttgattttc agccttattt attgctactt
    14881 ttccttcatt taataaaagt gttatttctt ctaaaagttg attgttattt tctggcattt
    14941 attttccccc ttggttatat attttattta aataaagttt aatcccttaa gtagattgca
    15001 aagtttttag actatattta aatttgtttt ttatttttta tttttaataa atttgcattt
    15061 tcactaaata attattcttc tttgtttatt aaaataactg tgttgtataa tataatcttt
    15121 atcaacacag ttattttatt taatgcgctt attttattgt attttatgta atacagttgt
    15181 tttttatatt atatgtccat tatttttcta tggttattac atatagattt ttaattatat
    15241 ttgtaccatt aatttaaaca ttcctatact actgcttaag ttttacacct ataccagctt
    15301 gaatatttta tttttatttg cttaaatagt tcaactatac tggtttaaat aggttatctt
    15361 tatttgctta aatagttcaa ctatactggt taaataggtt atctttattt gcttaaatag
    15421 ttcaactata ctgatttaaa taggttatct ttatttgctt aaataagttc aactatactg
    15481 atttaaatag gtttccttta gtttaaataa ttcacctata ctggtttaaa taggtttcct
    15541 ttagtttaaa taattcacct atacctactt gaatagttca cttttatttg cttaaattct
    15601 ataccaatac tggcttaaac attatactac catgattgta ttgtctatct tcaactacaa
    15661 taaaaacagg ttctaaatct ttagtagatg ttatttttaa ttctaaaaaa atattatcag
    15721 ttggtaatat ttttacatca taattattta aattattatt cgtgatacta ttaaatacat
    15781 caccttgacg ttcaaaaaaa acattatcaa ctattacaaa ataaacatct tcttttctac
    15841 tattttcaac ctctatagta ttttgtttag ttatatttag tctcatcttc cacctcctat
    15901 taatattact taattatatt aatacttgta attgttgcat tatgttcatc acaaggaaga
    15961 ggtttaaaaa caactatctc tgctaaataa ttctgtggag gatttgtaac tgaagtagca
    16021 ttgcaaataa atttaaaatt tgttcctgct acaacttgag tagaaacaat aagtggttca
    16081 taaccaaccc cttctaacat tcctacagct tcattaaata cttttaaatc ttcttcttcg
    16141 acagctctga attttgacca tccaccaaca aaaagactca tataaaaacc tcctttatat
    16201 atatatttat ttatatatag tagtaccaat tttctcaata ttcaatattt tctgtgtaac
    16261 tggtcaatga aaatgcataa ctggataaaa atacttataa aataacttaa ctttacatat
    16321 aaatagctta ctttacatgt aataagatta ttctacaata tgaaatgcat cttgttttga
    16381 atatattcta aatacaataa tggatatttt caattattat aattagaaaa tatccattac
    16441 ttaacttgtt ttatttaaaa tcacatagag actttattat tttttttatt gcatgaatag
    16501 ctatttcagg tatcataagg tgatataccc cctccttgaa accacaaaaa acatgacaaa
    16561 ctttacgaat cttgtcttaa aaaactacag gtacattcct tatttgtgga ttggagtagc
    16621 cataacaagc aaattttaca atttttatga tgacaaaaat caataaaatc gcttagtata
    16681 taacacgaaa tactacttgt tactattaag aatagttaga taggaagtat gcattataca
    16741 aataaaggaa tcaataaaga aagttaaaac atccctacaa attaaaaatc tgttacattt
    16801 ttctatattt aaacgttatt tacttacaat tggagaaata acaggttttc cttctcggtc
    16861 aagtaaaggc gtaaatccgg ctgcattgcc ataatagtgg aatacatagt tcacacctgt
    16921 ttctttatcc acccaaatct ccgttgcatt tattttccct tgtgaatata ctttttcaaa
    16981 tctatctttt cccattttat aatcctccat atttttgatt ggtctatgcc ttcttcactt
    17041 aagtagaaaa aatacataac ttattttttt aattctaata tgagcacgta aaaatgtcaa
    17101 acagcttatt taatttcata acttcatgtt actgttcgta accttgtctg tttacttaca
    17161 ctgcattaca gcaccatttt atcctttaca ttattgtatt gcacacagta aaaatgctta
    17221 ctttatggga tttttcaact aaaaaaagga ttttatctcc acaaaacatt tactatttgt
    17281 tacaatatac ctaattttaa tctcatatca acatttaatg aagatataac ctttttcaaa
    17341 tattgtaaag actccttaaa aatattagaa aaagattctg caaatctctc aacatctcct
    17401 ttttttaaat acctaagtat ctcaaaacat acatcaaaac gtgttttcaa aatttcatta
    17461 tattcttcat gaaaaaatct ctctaaatag attccaaata tcatatattg ttttaaatgc
    17521 aaaaatgttt cctgtaatac ataaatttgt gtataatcta ctatcttaca aagtaaaaca
    17581 aaatctggat aaaccttaca ctcttcacaa ttattttgta agtaccttaa atgagtttct
    17641 attttatcta cagctatcaa atccatttta tttcctagta aaagtactac atcctcaaga
    17701 gtaatcgtaa caaactccat agcatccaaa agcaaactca accttttagc tccttcttct
    17761 gtatttatat aatcatcatc atattcagaa aaaagcgtta catatgttcc cttgccatta
    17821 attgttttag caattcccaa ttcatttagc tcattatagg cacttctaac tgttggaagt
    17881 gatacattat atctattaat tatcgaagta attgatggta gacaatctcc tattttaata
    17941 ttgccttcaa aaatttgaca aattacatct gaaacaatta agtcatataa ataacatttt
    18001 tccaacgtta cagaattaaa actacttgta tctatacttt gtggagcacg attttctacc
    18061 attttaagta tggattctct acaagcttta tacatatatg ttaatagttt tttaaattca
    18121 attagattcc catccaatcc atataagtaa gtttttctta cctgctctat gtatttatta
    18181 cgaaaaaagt ctatacaaca attatccaag ttatccaagt tatcgaaatt ataggccaat
    18241 tgtgaaataa tgacatcatg ctgaataatc tcacaaaaat ggtgaatcag gaaattattt
    18301 gttttactta ttagtttatt tatatatttt agcctttgtt ctttaaagac taaataatta
    18361 gaactatttt tttccataca atcgataatc tcgtttaatt cttcaaagtc tttagatgta
    18421 aatcttttaa tattgtacat agcaatagaa ggaaatatta aactaaaaat cttatatatt
    18481 tctgatattt gtaaaatctt ctcatataat aagtcagatt ctattgattg tttactcgta
    18541 ctctggatta aaactggtct agaaccagct tttgtctcta taaagccctt ttctgagagc
    18601 atttttatta cagtttttat agtatttttt gataccatat attcttctga catgcttttt
    18661 atagaatcaa gcctatctcc aatagtaatt ttaccagtat aaatttgttg aattatatgc
    18721 tcaaaaataa ttaaatattg tggttttctc ttcatatttt tcctcttcga taaattagat
    18781 ttattgtttt ttgtaaggta tatttacttt tatttaatag ggcaaaatag ttgcttattt
    18841 tttcctcata atctataatt aatataaacc atttatatat atggtctaag ttttataata
    18901 ctgtaatgca gtaaaatagc atgttgtagc tatactacag caaattaaca taccattgta
    18961 gtatattaat tggaataatt ttaagtactt caaaattcca gcaaaacata tatgtgtttt
    19021 tgatattatg tatattcgtt aatagaatag ctatacttgt tttacataat aaattatatg
    19081 atacttgcta tatgtaatca ctatatttaa tacacataac tattatactt gctatatgta
    19141 attactatac tttatacaca tagttatcat atatgctata tgtaattact atactttata
    19201 cacatagtta tcatatatac tatatataat tactatactt ggacacatag ttatcataca
    19261 tgctatatgt aattactaca cttggacaca taactactat attt
//
LOCUS       DAYAOY010000080        19193 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYAOY010000080 DAYAOY010000000
VERSION     DAYAOY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.080"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            173..2032
                     /locus_tag="ACGI6B_002846"
     CDS             173..2032
                     /locus_tag="ACGI6B_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9752280.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2324..2923)
                     /locus_tag="ACGI6B_002847"
     CDS             complement(2324..2923)
                     /locus_tag="ACGI6B_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9752281.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3026..3823)
                     /locus_tag="ACGI6B_002848"
     CDS             complement(3026..3823)
                     /locus_tag="ACGI6B_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9752282.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3936..4041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4133..5119)
                     /locus_tag="ACGI6B_002849"
     CDS             complement(4133..5119)
                     /locus_tag="ACGI6B_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFG9752283.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5283..5459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5603..7333
                     /locus_tag="ACGI6B_002850"
     CDS             5603..7333
                     /locus_tag="ACGI6B_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFG9752284.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7443..7523
                     /locus_tag="ACGI6B_002851"
     tRNA            7443..7523
                     /locus_tag="ACGI6B_002851"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7477..7479,aa:Leu,seq:tag)
     gene            7858..8373
                     /locus_tag="ACGI6B_002852"
     CDS             7858..8373
                     /locus_tag="ACGI6B_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HFG9752285.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8865..8969
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9179..11653
                     /gene="gcvPA"
                     /locus_tag="ACGI6B_002853"
     CDS             9179..11653
                     /gene="gcvPA"
                     /locus_tag="ACGI6B_002853"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFG9752286.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11653..13110
                     /gene="gcvPB"
                     /locus_tag="ACGI6B_002854"
     CDS             11653..13110
                     /gene="gcvPB"
                     /locus_tag="ACGI6B_002854"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFG9752287.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13533..15890
                     /locus_tag="ACGI6B_002855"
     CDS             13533..15890
                     /locus_tag="ACGI6B_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFG9752288.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15976..16293)
                     /locus_tag="ACGI6B_002856"
     CDS             complement(15976..16293)
                     /locus_tag="ACGI6B_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFG9752289.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(16877..18148)
                     /locus_tag="ACGI6B_002857"
     CDS             complement(16877..18148)
                     /locus_tag="ACGI6B_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752290.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         7195 a         2261 c         3037 g         6700 t
ORIGIN      
        1 agaaaaaaat ttttgtatat ataaaggact ctaaagatga actatgtaaa acaaggttta
       61 tatattctaa ttgtatagaa aaatttattg taaacgcatg tatattatta gaaaaaatag
      121 tttggaggta ttcaataaac ataaaaaaca atatagggga taaaggtgtt ttatgaagag
      181 atttttgagg agaattatct tagttttatt tattttgttg atttttataa gttttatttc
      241 tattaaatta attcataatg ttggagatta tggaaaattg attaattatg ttgggattgt
      301 tcgtggagct agccaacgtc taactaaact agagatgaat catcaaccaa atgatgaatt
      361 aatagaatat attgatgaaa tcctacagga gttgattaca gggcatggag attatggttt
      421 agtaatcact gattgtaatg aatacaatga ggacttactt ttattagaaa aaaagtggga
      481 agatttaaat attgaaatta aaaaagtaag aatgaaagaa cagaataacc agttattaag
      541 tataagtgaa gagttttttt ctcttgcaaa tgatacagtg tttaaaatag aaaacttttc
      601 taaagaaaaa agtaattact taatgacttt aataataatt atatctatta taggtatatt
      661 agcatgtatc atattgattt tacaatatag taaaaaaatg gttagactgg aaaaattaaa
      721 tgtagattta aaaaatattg catataaaga tgaactaaca ggagtaaata ctatagaaaa
      781 atttaaattg gatgcaaatc aaaatatttg tatacataag gataagaaat ttgctgtatt
      841 ctatattgat tttgaaaact ttaaatacat taatgatata tttggatatg attatggtga
      901 catgatactg aaaagatatg ctaatcttat gatgaatgac atagggaaat atgaaatatt
      961 tgcaagagaa attgcagata gatttgttgc gttaagatgc tatgtagata aggaagattt
     1021 agtggtaaga caacgagatg tagacaatga actaattaat ataacaaatg aaattaaaaa
     1081 taaacatagt attactgttg ttagtggaat ttgttgtatt gaagatgtaa atgaaaaatt
     1141 aagtatagat ggtttaataa atagagcaaa ttttgcacaa aaaactgtta agaataaacc
     1201 aggtactaat tatgcttttt ataatgacag tattcgtaaa aaaatgattg aagaaaatac
     1261 tatcaaaagt agaatacatg aagctattga aaaaagagaa tttatagttt atttacagcc
     1321 taaagttaat ttacatagtc aaaaaataaa ttgtgcagaa gctttagttc gttggcttac
     1381 tccggataaa ggtattatat ctccagcaat atttattcca gtacttgaga agaatttttt
     1441 tattgcctta gtagataagt atgtttttga ggaggtttgt aaatggatta gaaagaggtt
     1501 agatgaaaat aaaccttttg ttcagatatc agttaatgtt tcaagaatac aattttataa
     1561 cactaaattt gtagaaactt attccaatat acaaaataaa tacaggatac ctaaaaatac
     1621 tatagaaatt gaatttacag agagtgttgc ttttgagaat caaaaacatc ttttggaaat
     1681 tatacatgat ttgcatgaaa atggatttac ttgttcttta gatgattttg gaaaaggtta
     1741 ttcgtcttta agtgttttaa aagatttacc atttgatgtt ttaaagcttg attctatgtt
     1801 tttcaaggct agtttagata aagataaaga gaaaatagta ataaaaaata ttgtacatat
     1861 gttaaaagaa cttaatatca caactgttgc tgagggcatt gaatgcaagg aacaagttga
     1921 atttcttagg gatattgggt gtgatttagt tcagggattt gtattttata aaccaatgcc
     1981 aatattagaa tttgaaaaaa tattggataa agagtttgtt tataatagct aaaatgtact
     2041 ttataaaata tgaactaatt aagtaaaatt attttgtact gaataaattt ttactttatt
     2101 tacagaataa tgattattta cagaaaagcg cgaaaagtgt attcctttaa gtgtgggatg
     2161 gatagcacaa atagaggaga aaaaatattg gccatataag aatgcataat aatataaata
     2221 tttaaaaggt cagcattata taaaaattaa aaagaaaata aataattttt ctataaaaaa
     2281 gagactatga gaaaatttac tcatagtctc ctaaataatg tatttatttt cttcctgcaa
     2341 cccattttaa tccccaatga aattcagtat ccatttctct atgacctttg aagtatttaa
     2401 ctactttttc aacactgaat gtctcatcat ttacattttc tatcaatgct attgaacaca
     2461 tattatagcc atttttatgt tcatcaagtt tgacaactaa gtctgaatct tgtttttgtt
     2521 tgattgttac aattccatca gcttcagacc agttagctac accttcgtat ataaaagcat
     2581 aaataagtat tctttttata tcttttattt tattaccatt tattcttaaa ttttctcctt
     2641 gtgtattact tcctgttctg tcatctccat ctaattgagc aaaaggtgga ttgtctaaac
     2701 taccaaaggc atttcctaat gcttgaacag caccttttac accatttttc atctcaaata
     2761 ggcaacccaa atctaaatct atattactgc ttcttaaaga gccaaaaaaa cccttttttt
     2821 gtactttttg attccaattt aaattaacta aaatttcacc taaatcgcta ttttcctttt
     2881 ttgtaaggtt tattttttgt ccctttttta attctatagc catagtaatc ttcctccaca
     2941 aacatagatt tatttaaatg gtagcactta ataagtcaat attcaacaag attagaataa
     3001 taatattaaa attatactaa atatactaaa aagtaggaat cacaatacct ttgtattttt
     3061 cttcaattaa gcttcttgct ttatctgatg taagaacatc ttgtagaact ttaatctttt
     3121 cactgtcttt atcttcagtt ctaaatgcta aaagatttgc atactcgtca tttataggag
     3181 ctgtataaat tccgtttttc ttggcatcaa gaccagcagg tacagcataa gtagtatcta
     3241 taaatgcagc agtcacatct ggaagagaag atggaatttg agcaatatct aactctttga
     3301 attgtattcc ctttggattt tctattatgt ccttaggact aactaatcca tcccctttag
     3361 gtaacgttaa aagtccaata ctttcaaggt atctaaggtt cttagaacaa gaagatgggt
     3421 tattgcttat tgcaattgta tctccttttt taaattcatc aacagatttt atcttataag
     3481 agtaaagtat tgctgggcat acatatagtt ttttaccagc cattattttg taacctttgg
     3541 ccttaacagc ttcttttaga taaggttcat gttgaaatag atttgcatca atttcaccat
     3601 tatttaaagc ttcgtttgga agtatgtaat catcaaagtt tttaacttct aaagtatatc
     3661 ctttttcttt aataagtggt tttaattctt ctaataactc tcctcctgga actaaagtag
     3721 caccaacaac aattttttta tcatctttag ctttagaaca tccaaccaca gctaaagtta
     3781 gtactagaca taataacaaa cataataatt ttttgaattt catataaatt ccctcctgaa
     3841 tataattgat tagttaaaaa taaaatacca ttctttatca cactgcaaaa taaaaataag
     3901 cctgagttta aactcaggct ttaaaagcta atttagactc atctttcagt ataatactgc
     3961 aggatttagc accaatgtat ataaaaatat actggttgct gggtttcata gggccagtcc
     4021 cttcaccact ctggataaga agtatcaatt tttaattatg ttataaatat tatcatgatt
     4081 ttttacgtct gtaaacataa ttttataaat attttaaaat ctttaatgta ttttatttaa
     4141 cacctaaaat tccagaaact agaatatcag tatcagcacc taagaaatag tcatttattt
     4201 caatagaatc cagagtattt ctcaaagcat tttcttcatg ccttatacca ataagtttat
     4261 tttctacaaa tgcaacatca cattttccaa aaaaatctcc aaaaaatttt atactagtta
     4321 ttatgccttt atcaacattt aaattaaact caacatttcc accagaatat tttaattcat
     4381 ttgaaagagt atattttggt gaattaccaa aattccaatc ccaagtttcg tattttgatt
     4441 tagtaagctt ttctatttgt tttatatcat gctcactcaa acaataatta gtattatcag
     4501 tatttgtttt agaaagataa tccattatag catctttaaa ttctaatatg tccatttttg
     4561 aatgtaaatg ttcacttata ttggttacac gagattttac agattttatt ccttttcctt
     4621 caaattttat aggtttaacc tttaatgctt ttgagaggtc atttatttca gatgaaaata
     4681 aaagagtccc atgatgcatt actttgtttt tgtagttgta ttgagcattt ccagaaaatt
     4741 tcttgccatc tataagaagg tcatttcttc cagaaaattc ggcattgaca tctagtgttt
     4801 taagtaaatc tattatgggc tgagtgaatc ttctgaaatc actaaaatta ttattgttac
     4861 aagctataaa tgtaaaattt atatttccca aatcatgaaa tacagctcca ccaccagact
     4921 gtctcctaac gacaggtata gaatattctt ttatatattc taaatttatt tcagaaagtg
     4981 tgttttggtt ttttccaaca attatagaag gttcattcct ccataaaata aataagtctt
     5041 catcaaaatt ttttagaaga tattcttcca ttgctaaatt aaaataaggg tttgtttttt
     5101 cattataaat taataacatt aaagtagttc ctccatagaa ttgataaaat ttttattatg
     5161 aatacaatta attttataat tcgacaataa tataaaaact cctttaaatt agaaatttta
     5221 aaaactacct tgcaaagttt aaataaataa tatatactca aaatatgttt aataaaagtg
     5281 aatagaaggg tagaggcgca ataatctatc agtactaata aggagttaag cacagagagt
     5341 tattaggaaa ggagatattg ccgaagtttt ataatcgatg ctttaagatt atattgctgg
     5401 gggtacatag aaaatatgta caactgtcac aggtttattt gtggtgagct atcatttaag
     5461 gaattatatt atatttattt ttactattgt tgatatattg taatgtttaa gccttgagtg
     5521 tttaccactc aaggcttttt tagttttatt gtaacataat accctgtaca gttcacaaaa
     5581 ttaaaaggag agggagattt atatgaggaa caaaaaaatt aatattattt ttctgacgac
     5641 aatcatgttc ataatgtcaa cagtaatggt ttttgcagaa gaagatatag acactattgc
     5701 actagccaat gcagaaaaat ttgggatttt aactttaata ccaccattag ttgctataat
     5761 actcgcattt ataactaaaa acgtaataat ttctttactt ataggtattc tttctggtag
     5821 ttttataatt aaagctagtg gtattaatgt ttttgcgaca tttatacagg catttttaga
     5881 tttagtagat agagctttag tatctttagc agacccttgg aatgcgggga taatactaca
     5941 ggttttgtca attggaggag taataaatct tgttgctaaa atgggaggag ctaaagctat
     6001 agctgaagca cttgctaaaa gagcaaagtc agcaaaaggg acacagctta taacatggtt
     6061 tttggggctt ttagtattct ttgatgatta tgccaattca ctaatagtag gtcctatgat
     6121 gagacctgtt gcagataaaa tgaaaatatc gagagaaaag ctagcattta taatagatgc
     6181 cacagcagca ccagttgctg gtttagctat aatatctaca tggataggtc ttgaggttgg
     6241 tttaatacat gatgcttttg aaagtataag tatagatgta gatgcatttg gtatattttt
     6301 aaatacaata ccatttagat tttacaatat attaatatta gctttcattg taatatcagc
     6361 attattatta aaagaatttg gtccaatgag aaaagctgag ataaaatcta gaagtagaaa
     6421 aattagtata gaccttgatg aaggcgtaga agagttggat gatttagctc caaaaaatgg
     6481 agtaaaattg agtgtatgga atgctataat cccaatagga acattgataa tagttgcatt
     6541 agcatcattt tattatagtg gatatacatc tataatggga ggagacgata aggctctgat
     6601 acaactattt acaaattctc catattcttt tgaagcaata aaagaagcat ttagtgcatc
     6661 tgatgcatct agagcattat tccagtctgc acttgttgca agtttagttg ctattataat
     6721 ggctgtagta aaaaagattt ttacaatatc agaagcaatt gatgtttgga tagatggaat
     6781 gaaaagttta gtaatcactg gtgtaatatt aatacttgca tggtcattaa gttcagtaat
     6841 aaaagagtta ggtacagcta aatttttaat acacctacta tcaggttcac taccaccatt
     6901 cttattacca agtttaatat ttggtttagg agcaataata tcttttgcta ctggaacagc
     6961 ttatggaaca atgggtatac ttatgccact tgcaattcca ttagcatact cattaaatcc
     7021 tgatatgtct tatgtaatag taagtactag tgcagtttta acaggtgcaa tatttggaga
     7081 ccattgttct ccaatttcag atacaacaat actttcttca atgggagctg gctgtaatca
     7141 tatagaccat gttaacactc aaatgccata tgctatattt acagcagtaa tcactattgt
     7201 atttgggtat ataccagctg gacttggatt gccaatatat atagtattac cagtggcaat
     7261 agctgctata tttgtaggta ttcaaataat tggtaagaaa gttgatgaag cggaaataga
     7321 acttgtagaa taaagatata aataagtttc accttttatt atcactattt tgttagtttt
     7381 tagataattt taaaaagtta ttgaaaataa tggcgaaata tagtaaaata aaagagtgat
     7441 aagcggatgt ggcggaattg gcagacgcac tagacttagg atctagcgcc attggcgtgg
     7501 gggttcgact cccttcatcc gcactttaca gtacttaatt gtactgaaaa aaactggggt
     7561 tgctggcttt cggagtgggc tatcccgttt ttattttttt gtatatataa tagattggca
     7621 ctttagtata aggtgctttt tttataggca aaaacagtac atttatttct gttttattat
     7681 cattcatcta ttagaatata ctttagttaa gaatgtgtta ataagcagtg ttcacattct
     7741 tgataaaatg tggaataata tgtattttta ctaagacgga aatgaaataa ttaaaccatt
     7801 aaataatatt ataagatgat gaataagtag ataaatcaaa ttttgtagga ggatattatg
     7861 gaaaaatata tatggttgtt tccattactt tttatttttc atgatatgga agagataata
     7921 ggttttggaa tatggcttaa aaaaaataaa tcaatgcttg ataagaagta tccatttatc
     7981 agtaaagttt atgaaaatta tagtactgaa ggcatggcat ttgctgtgtt tgaagaattt
     8041 attttatgca ttattttctg tatacttaca gttataacag agaatcagta tgtatatcta
     8101 ctatggttag gtagttttat agcatatacc ttacatttgg taattcatat agggcagtca
     8161 atcataataa gaaaatatat accttcactt attaccagta tcatatgctt accaataagc
     8221 atttggtgta taagtaagag tatttatata gttgattgtg aaatgagtac aacaattctg
     8281 tacagtataa ttggcattat aattgtagca ttgaatttaa agtttgcaca gtcgctgatt
     8341 ggaaaattta caaagtggat gtcaaacata taaattttag tttattggac agttcaagga
     8401 atcagaaatt ggaattttat agtattaata ttttatggta ttaatataat gtataaaatt
     8461 ttatagcaat gtatttcata agtataaagt gactagaatt ttaagaaata tacaaattct
     8521 ataaaaacta aataatttca agtatatttt aaaaatctta tttttcacaa gtttaaaatt
     8581 gtaaacgatt tcatcaagtc tatgaatata cttttaacat gttatagtaa atctcaatat
     8641 aaatttacat ttggttcttt gtgtgttatt ttaataataa attaagtgaa aatgttattt
     8701 tacattaata tataatatct ttgcataaga gtgaataata taaaagagaa ataattgata
     8761 ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata aatataatgt gttgacattt
     8821 aattgaaata ttcatacaat aaaattgaaa ataaatattt ccaaatgaga ttagcgagag
     8881 agtcctttat acgaaaaggc gccgaagaag aaatttaaga aatgccaatt tttagagaag
     8941 ctttcaggca aaaatatcgc taattgatgg gactctggag agacttaagt taagatacta
     9001 taagtcaccg aagaagcaaa ataatattaa attattaaat ctttcaggta tacaaacagg
     9061 gatatatagg ctaggattat tttccatgct gatatatctt ttttttatga aaaagtacag
     9121 gaaataatat caaagttact taaatacagt aggtctaatt ttctagggag ggttatatat
     9181 gaatgaattg aaaagagtat ctttgtacaa tatacacaaa gagcttggag caaaacttgt
     9241 tgaatttgct ggttgggaaa tgcctttaga atatgagggt ataaataaag agcatgagaa
     9301 ggtcagaaaa agtgcaggta tatttgatgt atctcatatg ggagaggtac agataaaggg
     9361 agcagagtca gaaaaattta tacaaaacct agttacaaat gatataagcg cattaaagat
     9421 aaatgatatt atatatacac ctatgtgtta tgaaaatggt ggagtagttg atgatttatt
     9481 aatatataaa tttggtgaag aagattattt attagtaata aatgcaggta atatagataa
     9541 agatgtagca tggataataa aacaaagtga aggatacaat gtagatataa aaaatatatc
     9601 tagtgaagtg agtcaattag caattcaagg acctaaagca gaagaaattc ttcaaaaaat
     9661 cacagatata gacttaaact caataaaatt ttataagtca attccatcaa caaaagtatg
     9721 tggttgtcct tgtttagttt caagaacagg atatactggg gaagatgggt ttgagatata
     9781 ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt ctaaaggttg gaggagaaga
     9841 tatatgtcca gctggtctag gttgtaggga tactttgaga tttgaagcag ctctacctct
     9901 atatggacat gaaataaatg aacacatatc tccaattgaa ggtggtttat ctatctttgt
     9961 gaaaacaaat aaagaatcat ttattggcaa atcaatttta tcaaaagaaa aagaaagtgg
    10021 agctaaaaga aagttagtag gatttgaaat gcaaggtaaa ggaatgccta gaaatggata
    10081 tgatataagg attggtgata aaacagttgg atttgtaaca acaggatgtg catctccaac
    10141 gacaggaaaa attttaggta tgggaattat agattctgaa tatgcaaaag taggaaatga
    10201 aataggtata gcaatcagaa aaaaagttgt tcctgctgtg atagttaaga aaccttttta
    10261 taaaaaacaa tataaaaaag ataatattat attaaacaaa gaaaataaat tttcatatat
    10321 acctgctaca agtgaagata aatcaaaaat gttaaaagta gttggattaa attcagttga
    10381 tgaattattt tcagatatac cagaagaagt aaaattaaaa agagatttaa atttggaaat
    10441 tggaaaatct gaattagagg taagcaaaat agtaaaaaga ttgtcagaag aaaatttaag
    10501 tttagaagac ttgacttgtt ttcttggtgc tggtgcatat gaccattata taccatcaat
    10561 tataaaacat ataacttcaa gatctgaatt ttatacagca tataccccat atcaagctga
    10621 aataagtcag ggaactttac aagtagtttt tgaatttcaa tctatgattg cagaaattac
    10681 aggtatggaa attgcaaatg catcaatgta tgatggagca actgctgcaa tagaagcatg
    10741 tataatggca atgaatcaga ctagaaaaag taaaatagta gtgtcaaaaa ctattcatcc
    10801 tgaaactctg tcagtattaa gaacttactt acaatataaa gattgtgaga tagtagaaat
    10861 tgacttttgt aatgaatatg ggactacaga tatagaaaaa ttaaaagctt ctgtagacaa
    10921 agatacagca tgtgttctta tacaaactcc taatttcttt ggaattattg aagaaatgga
    10981 agaaatagaa aagataactc atgaaaataa ggctatgtta attatgagtg tagacccaat
    11041 atcattagga gttttaaaaa caccaggtga gataggtgca gatattgttg ttggagaagc
    11101 tcaatcactt ggaaaccctc ttaattttgg aggtccttat gtaggatttt tagctagcaa
    11161 atctaaatat actagaaaaa tgccaggaag aatagttggg caaagtttag atgtagaagg
    11221 taaaattgca tatgtattaa ctttacaaac tagagaacag catgtaagaa gagaaaaagc
    11281 aacttctaat atatgttcaa atcaagcttt aaatgctctt gtagcttcaa tatatatggc
    11341 tactatgggt aaagaaggat ttaaagaggt tggtatgcag tctatgaaaa aagcacatta
    11401 tacctacaat aaacttgtac aaactggaaa atataagcca atatttaaag gtaagttttt
    11461 caaagaattt gctgtacaag gaaatcttaa tatagaaact ataaatgata aactcttaga
    11521 agaaaatata ttaggaggtt ataatttaga atacaattat ccagaattaa aaaattcaac
    11581 tttgctttgt gtgactgaga aaagaagcaa agaggaaata gataagttag ttggaataat
    11641 ggagggatta taatgaaaga atacaatagt ttgttgatag atatatctaa aaaaggtagg
    11701 aaagcatact cacttccaaa gttagatata gatgatataa aaatagaaaa tatgattgac
    11761 caagatatgg caagacaaag tgagttgaat ttgccagaag ttggtgaatt agaattagtt
    11821 agacattata cattattatc aaataaaaac tttggagttg atacaggttt ttatcctttg
    11881 ggttcttgta ctatgaaata taatcctaaa ataaatgaag atatggcagc acttccaaac
    11941 tttactggaa tgcatccata ccaatcttcg gatacagctc aaggttctct ttctttgatg
    12001 tatgatttat caagaagact tgcagaaatt acaggtatgg atgaagttac actacaacct
    12061 tcagcaggtt ctcatggtga gtttacaggg cttatgatta taaaagcata tcatgaaaat
    12121 agaggtgacc ataagagaac caaagtgata attccagatt ctgcacatgg aacaaatcca
    12181 gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag catctgataa aaatggagct
    12241 gtagacataa atgcattaaa agaggttttg aatgatgaag tagcagctct tatgcttact
    12301 aatccaagta ctcttggttt atttgaaaaa aatataaaag aaatagctac tcttgtacat
    12361 gaagcaggag gacttttgta ctatgatggt gcaaacatga acgctattat ggggataaca
    12421 agacctggag atatggggtt tgatgtggtt catcttaatc ttcataagac tttttcaact
    12481 cctcatggag gtggaggtcc aggagctggt cctgttggag ttaaaaaaga attagtacct
    12541 tttttaccaa taccagtaat tgaaagaaaa gaagataagt atgtactaaa ttatgataga
    12601 gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt ttggagttct tgtaagagct
    12661 tacacatata tactaactat gggaagggat ggacttaaag aggctagtga aatggcagtt
    12721 ttaaatgcta attatataaa ggaaagtata aaagatgatt atattttgcc aatagatacc
    12781 ttgtgtaagc atgagtttgt attaggtgga ttgccaagag gagaggccaa tataaaaact
    12841 atcgatattg caaaaagact actagattat ggataccatc ctccaacaat gtactttcct
    12901 cttattataa atgaggcact tatgatagaa cctacagaaa gtgagagtat agagacatta
    12961 gatagtttta tagaagctat gaaaagtgtt gctaaagagg caaaggaaga accagaacta
    13021 ttgaaaactg ctccacataa tactttggtc aaaagagtag atgatgcaag ggcagtaaag
    13081 aaacctatac taacatggtc acaaagataa atagttaaaa agtagagatt taaagtataa
    13141 agagaattaa atttgtaaga aaaagctatc taacttaatc tagtttagat agcttttttg
    13201 tatattagta tgtatggtat tttctatata aagttttaaa ttatattagt gcttatatag
    13261 aatattgata tgatgattat gtatagatat taataagaat tgttgaattt atcttgcctt
    13321 aactttattt actcttttat attaagaaaa acttaactaa aatatagcga aattcataaa
    13381 aatttaggaa atcttaagaa ttataatatt taaatcttta gatttatatt ttattatata
    13441 aactatagaa taataaaata aaaaaattga gaattttata atatatttgt agatttgagt
    13501 ataaaaaagt gctaagttga aaggagtaaa atatggataa ggttaattta gaaaggtata
    13561 gttgtgatat aaatgttggt ttaaataaaa atcaacttca aagtagaata gatgataatt
    13621 taattaatgc atttgataat ggaaaaacca aaacatataa acaaattttt aaagacaata
    13681 tatttacact ttttaatctc ataaatattg ttttagtctg tttattagtt tcagttggtt
    13741 cttttaaaaa taccttattt atatttattg ctgtaattaa tactattata ggagtaatac
    13801 aagagataag agctaaaaga ttattggaca atctatctgt tatagtttca aataaggtat
    13861 ctgtcataag agaaaaagag aaaaaagaga ttgatgtaca agaattagta ttagatgata
    13921 ttatgatttt aaaaacaggt aatcaaattt gtgcagattc aaaagtttta aatggaaaat
    13981 tagaggtaaa tgagtctttg gttacaggtg aatctgatat tattataaaa aataaaggcg
    14041 attttttata ttctggaagt tttgtagttt ctggagaagc ttttgtaaga gttgaaaata
    14101 taggaaaaga taactatgca aataagatag ctaatgatgc aaagatttct aaaaaacata
    14161 attctcaact tagaaattca ttaaatgcaa tattaaaaat tgtaggtata ataataattc
    14221 ctttaggaat tatattgttt gcaaaacaac attttggaaa tggagattct atagctacat
    14281 ctgtagtagg tacagtggca gctgtaaatg gtatgatacc agagggatta acattactta
    14341 cgagcattgc tctagctgta ggaactataa aacttgctag tcataaaact ttagtgcaag
    14401 aactgtactg tgtagagact cttgctagag ttgatacact atgtcttgat aagacaggta
    14461 caattacaga aggtaaaatg caagttgatg atatagtaat gttggaagaa gtggatatta
    14521 atgaaataat ggggaatata tgtaattctc tcaaagatga taatgcaaca ctaaatgcaa
    14581 taagagataa gtataatgtt ttagatacgt ataaagctaa gaatttaata ccattttctt
    14641 cagaaagaaa atacagtggg gttacctttg aaaataaagg tacatattta ttaggtgcat
    14701 ttgaatttat atttaaggaa aaaaataaaa aacttaggat agaagttgaa agatattcta
    14761 aaaaaggaaa cagagtaata gttttagctc atagtgattc ttatatggaa gataataata
    14821 ttcctgaaga tataaagcca ctagctttta tattgatatt ggataaaatt agagatgaag
    14881 ctaaggaaac tttagaattt ttttacaatg aaggtgtaga tataaaaatt atatctggtg
    14941 acaatcctat aacagtaagt gaggtagcta gaaaagcagg tcttaaaaca gctagtaatt
    15001 ttattgatgc aacaacactt aaaaatgata gtgatatata caaagcagtt gataaataca
    15061 gtgtttttgg aagggtaagc ccacaacaga aaaaacaaat gattttagca ttaaaaaaac
    15121 aaaatcatac agtagctatg actggagatg gtgtaaatga tgtgctagca cttaaagaag
    15181 cggattgtag cattgccatg gcatctggaa gtgatgttac taagaatgtg tctaatttag
    15241 tcttattaga ttcaaacttt gcatcaatgc caaaggttgt tatggaggga agaaaagtaa
    15301 ttaataacat tcaaagagca tcttctttgt ttttagtaaa gacaattttt tcattcttct
    15361 tgtcgttatt aacattattc ttcttcagta gacagtatcc atttgttcct atacaattat
    15421 cactcattgg ggctgtaaca gtgggtatac catcattctt cttagctctt gaagcaaaca
    15481 aaagtagaat ttcaggaaat tttttactca atatattaaa aaatgcttta ccaggagcac
    15541 tatgtgtgac agtaaatgta attattgttt acaatatagt tgaatattta ggttatagtt
    15601 caagtgtatt ttccacaatg tgtgctatat taactggtgt atgtggtctt ttaatattaa
    15661 gacaacaatg tttgccattt aataaagaaa gattattttt aatagtaagt atgactattg
    15721 catttgttat tgggattcta tttttaggtg gattattctc atttgtagca ttgagtagag
    15781 aattaattct cataactatg gcattagcaa tattaatgcc aattatgata aaagttatgt
    15841 tatttgtctt agatgagata ttagagacaa ttgttccaga tttaaattaa ttttaaaatg
    15901 atataaataa gagcagtttt agttgaactg ctctttttag atgtgtttaa ttttatgtaa
    15961 gaatatgtat ttttattaat cagctatata tgagcaacag tagtcaacaa cttcattaac
    16021 ctttaagtca aaacttgaat cactaggaac attaaatttt tgcccttctt tataagttac
    16081 aaactcattg cttccaggca gttttacatc catacttcca gcaagcatct ccattatttc
    16141 ttcttctcta gtagaaaaag tgtattctcc tggcaacatt aatcctaaag tttttctttc
    16201 tccattaggc agtattatta ctctgctact taccttacca tcaaaataaa cgtttgcctt
    16261 ctttactgca gtaacattct taaattcact cattagataa aacctccaaa attatttgta
    16321 catatatatt atataaaaat aatgtaaaaa atgctatata tttacaaaaa aatttagtat
    16381 ttattaaaaa aattaataag aataaaaaag agatgaaatt gcttaaattg ataaaaaata
    16441 taaaataaat tgcaaagtaa gcaagaaaag aataagttga agagttatat attataaata
    16501 ttaaaaattt tagtattaat aaaaaagata ttacattaat atgaaaatat aggagattag
    16561 aagtgtttga taattacagt acatgttaga tatatcattg taaattaaca tagctttgaa
    16621 tataataatg ttcagaaggc aatatttttg aggtctgaat ccaataataa tatagtctta
    16681 tgattgtaga tatcaaaaaa tgtaagttac taggaatacg ttttgtagat ttatatatga
    16741 ttaataacta aagctataaa taaacaaaat aaaaagagta tgtttcaacc tatagctttt
    16801 agatttaaaa tatactcttt tattaattat agttgtataa attaaccata atatataatt
    16861 aaaattttta ttataattat atattaggtt cttcaattag ttttccacat acatatctat
    16921 gaatgatatt tctatcatca ccaacatata taaatctttc aagtctttca agtatagaat
    16981 aattgtcatg atttaaattt gaatcatcaa taatcaatgc atcaaaatca taaccttctt
    17041 caaagctacc aacttttcca aagaaacttc caccactttt ggttgcaata taaaaagcct
    17101 cagataaaga taagaaatta tacttcttac cagagttaac ccaatataat ttagataatt
    17161 gtattgcaga taccattgct ttaaatattg acaatgtatg tccaccactt atatctgatc
    17221 caagtgctaa tctaatatta ttatcgatta attttctgat aggcatagca ccactaccaa
    17281 cattaaagtt agaagctgga cagtgaactg ccataacatt attttctctc attaaatcta
    17341 tttcatcctg agaacaatga acacaatgag ccattaaagt aggagtttga ccaaataaat
    17401 tatatctatt atatgcatct ccatagaatt tagattcagg ttctagactt ctaacccatt
    17461 caatctcatc ataattttca gataagtgtg attgaatagg aatattatat tttaaacata
    17521 aatctcctaa accttttaaa agttcacttg tacagcttgg tataaatcta ggtgttatta
    17581 ttggttttac aatagaatct gtatcactat acttatttat aatttcttct gtatcagtaa
    17641 tagaatcctg agtgttttct aataaagtat cagggcaatt catattcata tttactttac
    17701 caacgtatgc tccaagacca acttctttga atatatccat aagtttcata gaggcttctt
    17761 tatgtactgt tgcaaacact gctattcttg tagttccaga tttccaaagt tcttttacaa
    17821 attttttata catttttgtt gcatattcta tatctttata atttgattct tctggaaatg
    17881 tataagtttc aagccatggc aaaagctctt tatccatagc cattccaaga tttttaaatt
    17941 gagaggcatg ggcatggagg tcattcatac caggtattat aagcttatca gtgtaatcta
    18001 ctacttttag gtttttgtat tccttaggta tctctttaaa tattcccttt actttacctt
    18061 tttccagcat aatataacta ttttcaaata ctgtgaaagt ttctgaagtt tttgtaaaaa
    18121 ttatatttcc ttttagtact tttaacatat aggtctcctt agttttaaat ttgttttaat
    18181 atatgtaggt aaaattttgt tttacaaatc aaaattttac cacatgtatt atatcaaatc
    18241 taaactataa atactattag ataatagcaa atattagtga aatacagttg tttactatta
    18301 ttatatataa ctgacattta agtgacattt aagaaaaata taatgcctac ttacataaaa
    18361 tggaatgtta tttaaagaga actttgatta tattttcaga agttttttta tccatatcgt
    18421 ttaaaacaag aaaatatcta ttcatagtta tttttatatt agtatgtcct aatctttcag
    18481 agatgatttt tatattagtt ctagctagaa gaagaactat tagaatagat aatatagtaa
    18541 gtatttacaa atatgtaggt gttcttaaat tgataaatta ttccatttta attttatagt
    18601 ttgaatttta tgatataata aaaatatata aattttgcag tgagcgatat ttttgataaa
    18661 gtagggttta acagttgcaa tgtaagggat tgagggtgta tgataaatgt tatcaattgc
    18721 actactcatg gttcactgca aatttgagag agttgtatgt gtgtaagtac tgaaaatact
    18781 tagtttattt tggggtttta tattaactaa gtggtatgta aataaataat gtgttttatc
    18841 atcataactc ctgctaaaca tgttttatat taactatgtg gtatgtaaat cttgttaatg
    18901 caaataaaca acatagcact attaatagtt ttatattaac tatgcggtat gtaaataggg
    18961 tttctgataa aatcttcaaa catgtaaaat atgtgtttta tattaactat gaggtatgta
    19021 aatcttatac ttagttagaa ctatatatcg acacaaatat gctttatatt aactatatgg
    19081 tatataaatt gcaattttta ttcgttgtcc aatctctttg aaatttgttt tatattaact
    19141 aagtggtatg taaatttgta acaagtatag gtaaaatacc ccaataattt ata
//
LOCUS       DAYAOY010000081        19074 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYAOY010000081 DAYAOY010000000
VERSION     DAYAOY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.081"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(208..1785)
                     /locus_tag="ACGI6B_002858"
     CDS             complement(208..1785)
                     /locus_tag="ACGI6B_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HFG9752291.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="ACGI6B_002859"
     CDS             complement(2082..4493)
                     /locus_tag="ACGI6B_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9752292.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4668..5084
                     /locus_tag="ACGI6B_002860"
     CDS             4668..5084
                     /locus_tag="ACGI6B_002860"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFG9752293.1"
                     /translation="MEENFLILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFL
                     LSNSLSWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLM
                     IDLFSLNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="ACGI6B_002861"
     CDS             complement(5149..7266)
                     /locus_tag="ACGI6B_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9752294.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="ACGI6B_002862"
     CDS             complement(7297..8004)
                     /locus_tag="ACGI6B_002862"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9752295.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="ACGI6B_002863"
     CDS             complement(8111..9982)
                     /locus_tag="ACGI6B_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HFG9752296.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI6B_002864"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI6B_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFG9752297.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="ACGI6B_002865"
     CDS             complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="ACGI6B_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HFG9752298.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            complement(15380..16978)
                     /locus_tag="ACGI6B_002866"
     CDS             complement(15380..16978)
                     /locus_tag="ACGI6B_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9752299.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            complement(17197..18798)
                     /locus_tag="ACGI6B_002867"
     CDS             complement(17197..18798)
                     /locus_tag="ACGI6B_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HFG9752300.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         5631 a         3570 c         2023 g         7850 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcctttt ttatcataca attactatta taccttaaaa
    12721 ttatagaata gtagacaatt atttttattt atagtataat aatcatactt aaataaatta
    12781 catctttaat tatttaggta tttctcacaa aaaaaggaac tatttttagt tcctttttta
    12841 atttcttttt ataaaaaaga gaacttcttc aaagaagttc cctcttattt atagtttaat
    12901 tttatcttat aattctaata aatcttttat tctcttaata actgaatctg ctattccttt
    12961 accaacttga gtcaatttct tagaatcatc agaatttgta actttactta ttgctactga
    13021 ttggtcagaa gataaactat cagttgctaa aactattgga gcttcattgt gacctgctac
    13081 tgctgctatt gctaacgcat ctactaattg atcttctttt gtagaaccat ctttagcaac
    13141 atagaagttt ttaacacctg tgctaattac actgtcacta ttatcttttt caaaataagt
    13201 ttttataact tctgcattag tatcttgtct atcatctcca ctaactcttt ctggactctt
    13261 acctgtagca tcatctatag catcttccat gtcttcagaa acactagatt ttccacctat
    13321 tatatctaca taagcacttc ctataaagtc ttttgcatca gttgataaat cttttccttt
    13381 tccatcaact actactattg gcatttcttt attagaagca actgatgcta tactcattgc
    13441 atctgctaat ccagtaccac caactacaaa tactttatta tggtttaaac ctatttcatc
    13501 agcaatttct aaagatgtag catatctatc atcaccagat aatctttcaa cttttaatcc
    13561 catatctttt aattcatttg ctacttcttt agatacagaa ttttccccac cagctatata
    13621 aacagtcttt ttagatgtta taccagtttt atcatctaat cccattactc tctttatttc
    13681 agattttact gatgaatcta acttatcttt tgaagttaat aataatggag catctttaac
    13741 agcagctaaa ggtgaagcta ctagaccatc tactattgat tgtgatccta ctaatactac
    13801 ctcattagct tctacataat ctcctttaat tcctccatct gcattataag agtctttact
    13861 tatttctatt gcagtctcta ttctatcatc accagctaat acagaattat ttgaataaga
    13921 attattataa gttcttaatt catctaaagc tgattttaag ttattcttat tagtagatct
    13981 taatgttatt atagcaggag tatctactct atcacttgta ggtaattctg gcttatagtt
    14041 agatgatgca tatgttgaag ctgtatttaa tcttttacct gttggataaa gaactacctc
    14101 ataataatca ttgtcagttt tttcaaaatc acttgctgtt actgtcttat atgcatcttc
    14161 taattcagta tctttaaata catatttttt agctaaagct tctgcttttt cttttgcatc
    14221 tccagatatt gtaactgttt catctgatgc tgctataact gttacatttg attcaactgc
    14281 tccaactttt tctccagttt tttcaaaact taattttgat gtaccatcag tagtattaac
    14341 cgttataact ggtttactaa aatcatatat ctctttagta ccatcaagag atgctactgc
    14401 tgtatctaaa gtaggtgcta ctgctgcttg tgtatcatat tttaatttaa ttgcaccagc
    14461 tactgcatca gcatatgttg ctgtaatagc tactgttcct gtagcattat taccagtctt
    14521 atcagtaaat tttgaagttt tgactgctgc ctttgtagct gctaaaactt catctttagt
    14581 agctccatct acataagctg acgtagttgt atcatatttt tgtaacttta atttagcatc
    14641 attttctatt gaatttccat cttgatctac aatcttaact gttgttgcag taccaacttt
    14701 agtaactaca gcattactat caagttcctt taataatgta tcatcatatt ttttttctgt
    14761 tccatctgta atttttacag atatttttgc atattctcca gcttttaaat ctttaatttc
    14821 atctttaaca taacttattt gacttgcttc taaacttgat atagataccc cttttggatt
    14881 tccttttttg tcaaaaaagt taactgttat gcctgttaca ccatctgcaa catatttttt
    14941 aagtattctt tctatatcac tatacttact actagataca gtggtatttc catttggaaa
    15001 actatcctct gctgcagcga aaactggtgc tgctgatccg attactgtaa cggcagacat
    15061 tacaactgcc aaatttttct tattcattcc ttaaattcct cccaacatta tattaattac
    15121 atctctttaa ttatgtacat taaaattcca tgttcatata aaccaaatat ttctcacact
    15181 cttatgatag actatataaa atagaatttc aataatttat gcaaaattaa tagaattttc
    15241 tttcaacatt tttccccttt tttttgctat ttcatatttt ttaaattaaa ttttcaattt
    15301 taattttatt aatacattta tttatctgat ttaatcaata tatattaata ttttctacca
    15361 taaaatatta atatatactc taactattta aaaattccat tgtagcatta acttctcttt
    15421 gattagaaac atacattcca aagccaaaac tataaaactc ttcctttaac atattatcat
    15481 tatacttaga agacttcttc cataaatcaa atacagactt agcaatctct tttgcatctt
    15541 tagatgtata tacagactta acattcatag gtaaatatgc aatattttca tcagttctca
    15601 ttccaaagcc agaaaatact tcagctgcat ttttcccatc tatttcatga gcaaatatct
    15661 tcttctcatc catatactta gaccatgcat ttgccattcc agctaaagaa tctaattctc
    15721 ttaaactttt cttacctttt tcttttctat atgaattaac tagattatac atttcattag
    15781 ataccatagc cttaaactca taactattag ggtcttttgt acttgtgttt ataattactt
    15841 tgacatttac attaatctca cacttttgcc caaaattatc aaaagcactt acatttaaaa
    15901 catacgttcc tgccttactg gtatccacag ttccctcaat attaggcttc aaattatttc
    15961 cataataatc agtggcaact atattcaaca tactattatc aaacttatcc cctacagaaa
    16021 tctctacatc attagcagtg attgtaggca ttgtattttt atcagatgca gaactaatac
    16081 attggtcagc aaccttctta tccattccac ctaaagaagt aacttcatca gcacctctaa
    16141 gcacactctt atcacttcca tcattaacaa gtactatagg tttgtcctta gccaacggag
    16201 aaccagccac agcatcaact aacttatacc cttgagaaac ataaaacttc tttgttcctt
    16261 tataaaagcg ttgtataacc ttcttattag tttcaaatct atctttacca gcaatcctaa
    16321 cagaattagt cttactcaca agttcattac tcattatctc ctctgaccca agagaataac
    16381 attgaacgcc atcaacctta aatggtatac tctttccatc agtaagtata acaggaactc
    16441 catctctagc agctacagaa gatacactca ttgcatcagc ctcacctgtg tatccattta
    16501 caagaaaaac cttatcacca tcatttactg gtttaatagc tgaaatctcc ttagctataa
    16561 gatagctagt ctttattcta tcttccccac caattctttt aacttcaatc cctttattct
    16621 taagttgatt ttctacagat ttacctattg tatcctctgt acctataaca taagcattct
    16681 taacatcctt tagtcttttt tcaacatctg ttggtatctt atctctttgc actaacatta
    16741 taggagcttt tacaactcca gataatccac ttgcacttaa tccatctact atagaattat
    16801 cagtatttac taatatgact gtatcatata tttgtttatc tgctatcaat gcagaagtct
    16861 catatctatc tttcccttgt atcttttcta tactcgaaag tgcattgata tttggtgctc
    16921 ctgccattat taatgatatt gtcaatccta ttgataatac tctcttattt accttcatcg
    16981 aactcctcct actaattttt ttatataatt cagctaatca taaattacac cctcagtagt
    17041 gattccctca aaatttataa actactaaaa caaaaaacac ctaaaatacc ataacctgta
    17101 aagcaataat aaaacagatt acaaaaagta ctttagatgt gattttcatt ttataatatc
    17161 agtaaattaa caaaaaaaca agatttatgg aattttttac aagctacctt cattgccatt
    17221 taaaaactct tgagtagcat gcacttgacc atcagataat atataaagcc caaatccagt
    17281 actataaaac tcatcactaa gcatgtttgc attgtattct ggagatttct tccacacttc
    17341 aaatatagcc tttgccaagt cttttgcatc ttgagttgtt tgaacactct tagaatctat
    17401 ataaatatat gctatatttt cctcacttct cattccaaac tcagaaaata cttgtggagc
    17461 atttttgcca tctatataat gttcaaaaac tttgttatcc atcatatact tagaccatgc
    17521 atttgccata ccttctagct tactagatac taccaaaggc tctttacctt tttcttttct
    17581 ataggaattg attaagttat acatttcagt agataccatt tttttaaatt ctgggctgtt
    17641 gtaatcatga gatttatcat ccaacacttt tatttctact ctttttcctg tactttttcc
    17701 ccattcgtct gtagctttta atgtcaatat gtatgtacct actctatttg tatctataaa
    17761 cccatcaact tctattggta ataccttccc agtgttatct ttagctacta tatttaactt
    17821 gccagtgtca aacttttcac ccttatatac ctctgtactt ccaactgtaa ttgctggggg
    17881 ttgtccattt gactttgaag cacttataca ttgttgtatt accttctcat ctatttcacc
    17941 tacagaggtt atattcttag ctccttctaa tacagtttta tcgcttccat catttactag
    18001 taccattgga gagtttttag taagtggtgc agctgctatt gcatctacta attggtatcc
    18061 atctgataca tagaatccat ctgcactatt aaaaaagtag tctattacgt ttttgttagt
    18121 ttcaaatcgg tcagttcctt ctattcttac tgagtttgta tttttaacta gaggattact
    18181 cattatctcc tctgaaccta tacaatatga ttgaatattt tttacatcaa aaggcacact
    18241 ctttccatct gtaagtatga ttggagctcc atctctagta gctactgatg atacactcat
    18301 tgcatctgct tctccactat atgcattagt tagtagtact ttatcaactt tttttatagt
    18361 tgctatttct ttagctatta aatagcttgt ttttagtcta tcttctccac caattctttt
    18421 gacttctata tttttagaat ctagttcttt ttctactgat ttacttatag tatcttctgt
    18481 acctattata tatgcttttt tgatattttt tagacatcta tttgtgtctg ctggtatctt
    18541 attttgttgt gtaaatagta ttggagcttt tgtagctccc gataatccac ttgcacttaa
    18601 tccatctgca agtgattttt ctgtatttac taaaattact gtttcataag tctgtttatc
    18661 agctatcttt gctgctgttt catatctatc tttaccgtat attttttcta ctactgatga
    18721 tgcatttaca cttttgaagt ttactaagat taatgatact gctagaccta atgataatat
    18781 ttttttgttc attttcatat ccccctgatg atttttatat tttatattta tattgtgatt
    18841 attttccatt tattttctct ttattttcca tatattatag agtatatatg atatgaatat
    18901 atctcgtcta tatattagtg ttttatttta ttaaatttaa aaaattattt ttaacttctt
    18961 tttaaacata ttcaaaattg aatttatatt tatatatata aaagatatct gctttattta
    19021 aaattaaact taatatagct caaattatta tacattttaa cctcaatatt atta
//
LOCUS       DAYAOY010000082        18965 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYAOY010000082 DAYAOY010000000
VERSION     DAYAOY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.082"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            27..101
                     /locus_tag="ACGI6B_002868"
     tRNA            27..101
                     /locus_tag="ACGI6B_002868"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACGI6B_002869"
     tRNA            116..200
                     /locus_tag="ACGI6B_002869"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACGI6B_002870"
     tRNA            210..293
                     /locus_tag="ACGI6B_002870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="ACGI6B_002871"
     tRNA            317..391
                     /locus_tag="ACGI6B_002871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="ACGI6B_002872"
     tRNA            416..492
                     /locus_tag="ACGI6B_002872"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="ACGI6B_002873"
     tRNA            502..577
                     /locus_tag="ACGI6B_002873"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="ACGI6B_002874"
     tRNA            586..661
                     /locus_tag="ACGI6B_002874"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="ACGI6B_002875"
     tRNA            664..752
                     /locus_tag="ACGI6B_002875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="ACGI6B_002876"
     tRNA            756..831
                     /locus_tag="ACGI6B_002876"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="ACGI6B_002877"
     tRNA            838..911
                     /locus_tag="ACGI6B_002877"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="ACGI6B_002878"
     tRNA            926..1002
                     /locus_tag="ACGI6B_002878"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="ACGI6B_002879"
     tRNA            1020..1096
                     /locus_tag="ACGI6B_002879"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="ACGI6B_002880"
     tRNA            1105..1180
                     /locus_tag="ACGI6B_002880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="ACGI6B_002881"
     tRNA            1188..1261
                     /locus_tag="ACGI6B_002881"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="ACGI6B_002882"
     tRNA            1269..1345
                     /locus_tag="ACGI6B_002882"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="ACGI6B_002883"
     tRNA            1358..1433
                     /locus_tag="ACGI6B_002883"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="ACGI6B_002884"
     CDS             1509..1922
                     /locus_tag="ACGI6B_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752301.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="ACGI6B_002885"
     CDS             2231..5662
                     /locus_tag="ACGI6B_002885"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFG9752302.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="ACGI6B_002886"
     CDS             5904..7832
                     /locus_tag="ACGI6B_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFG9752303.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="ACGI6B_002887"
     CDS             8285..8749
                     /locus_tag="ACGI6B_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFG9752304.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="ACGI6B_002888"
     CDS             8756..9250
                     /locus_tag="ACGI6B_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFG9752305.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="ACGI6B_002889"
     CDS             9266..10291
                     /locus_tag="ACGI6B_002889"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HFG9752306.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="ACGI6B_002890"
     CDS             10278..12725
                     /locus_tag="ACGI6B_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFG9752307.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="ACGI6B_002891"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="ACGI6B_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFG9752308.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="ACGI6B_002892"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="ACGI6B_002892"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HFG9752309.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="ACGI6B_002893"
     CDS             15440..16534
                     /locus_tag="ACGI6B_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFG9752310.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="ACGI6B_002894"
     CDS             16663..18840
                     /locus_tag="ACGI6B_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752311.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         7725 a         2119 c         3618 g         5503 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaagagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aaaatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgagttag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agtataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcgttgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gagctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaataaag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gagagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaaattat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgagg ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagagccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagactaaag aagcttacaa aaataatgat
    17341 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tcgccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa atggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18421 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggcatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc ttacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18961 attct
//
LOCUS       DAYAOY010000083        18945 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYAOY010000083 DAYAOY010000000
VERSION     DAYAOY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.083"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(60..716)
                     /locus_tag="ACGI6B_002895"
     CDS             complement(60..716)
                     /locus_tag="ACGI6B_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752312.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1828..2238)
                     /locus_tag="ACGI6B_002896"
     CDS             complement(1828..2238)
                     /locus_tag="ACGI6B_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752313.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(2454..3836)
                     /locus_tag="ACGI6B_002897"
     CDS             complement(2454..3836)
                     /locus_tag="ACGI6B_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9752314.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(3847..4206)
                     /locus_tag="ACGI6B_002898"
     CDS             complement(3847..4206)
                     /locus_tag="ACGI6B_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752315.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(4411..5190)
                     /locus_tag="ACGI6B_002899"
     CDS             complement(4411..5190)
                     /locus_tag="ACGI6B_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFG9752316.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(5651..6262)
                     /locus_tag="ACGI6B_002900"
     CDS             complement(5651..6262)
                     /locus_tag="ACGI6B_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9752317.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(6354..7343)
                     /locus_tag="ACGI6B_002901"
     CDS             complement(6354..7343)
                     /locus_tag="ACGI6B_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFG9752318.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(7410..8273)
                     /locus_tag="ACGI6B_002902"
     CDS             complement(7410..8273)
                     /locus_tag="ACGI6B_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HFG9752319.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(8379..8975)
                     /locus_tag="ACGI6B_002903"
     CDS             complement(8379..8975)
                     /locus_tag="ACGI6B_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HFG9752320.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(9015..9818)
                     /locus_tag="ACGI6B_002904"
     CDS             complement(9015..9818)
                     /locus_tag="ACGI6B_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFG9752321.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(9854..10294)
                     /locus_tag="ACGI6B_002905"
     CDS             complement(9854..10294)
                     /locus_tag="ACGI6B_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9752322.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(10307..12409)
                     /locus_tag="ACGI6B_002906"
     CDS             complement(10307..12409)
                     /locus_tag="ACGI6B_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9752323.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(12670..14373)
                     /locus_tag="ACGI6B_002907"
     CDS             complement(12670..14373)
                     /locus_tag="ACGI6B_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFG9752324.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(14399..15358)
                     /locus_tag="ACGI6B_002908"
     CDS             complement(14399..15358)
                     /locus_tag="ACGI6B_002908"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9752325.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(15494..16012)
                     /locus_tag="ACGI6B_002909"
     CDS             complement(15494..16012)
                     /locus_tag="ACGI6B_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9752326.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(16055..16540)
                     /locus_tag="ACGI6B_002910"
     CDS             complement(16055..16540)
                     /locus_tag="ACGI6B_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HFG9752327.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(16537..17184)
                     /locus_tag="ACGI6B_002911"
     CDS             complement(16537..17184)
                     /locus_tag="ACGI6B_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HFG9752328.1"
                     /translation="MNAIIKGGVYIINYLELDDVHIDALREIGNIGSGNAITALASMI
                     NSNVEVLIPMVKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLML
                     SSLFNKEEAFLDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVG
                     AVLNVPMIRFSDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(17219..17581)
                     /locus_tag="ACGI6B_002912"
     CDS             complement(17219..17581)
                     /locus_tag="ACGI6B_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9752329.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(18325..18822)
                     /locus_tag="ACGI6B_002913"
     CDS             complement(18325..18822)
                     /locus_tag="ACGI6B_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9752330.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT         6132 a         3446 c         1779 g         7588 t
ORIGIN      
        1 aatattaatt taaaaagtta ttcctaattt aaataatact actatcttca tcagtgaacc
       61 taaactgcaa ctttagactt attagctaaa gccaatatta gacctaaagc tatagatgca
      121 aatgcagaag caattactct atactcagca gggaataaaa atacaattgt atgacatggt
      181 atccagaaaa atggtatagt ttttaaaaca ctaaaaccaa ggtaagcatt ccagtccaca
      241 atatttacca catctttaag ttttacctta atcccctttc caacctcata ttttttatct
      301 ataaaacagt ccataacctt atggaataaa aatacagttg gtgacattag tatattcata
      361 gttgcactct taaatagagc atttgcaaaa gctattccct caaatggaag cataccatcc
      421 acttgtgctt gacctacacc tgatgagtag attgacattc catatgtggt aactacacct
      481 attattcccc aaactattgc tctataaaat actcctactg gtattttcca atccttatac
      541 aaaattctaa caccaagtaa atctcccatt gtagctaata cagcaaactt taaaaaacct
      601 ccaatatatg gatgtgcacc tgttgcttcc ataaatactt ctctactact tggtattgct
      661 aagacagcaa taatacaaat aagaactcca ctccatataa aatcgccttt tttcatattt
      721 ataaatacta tttaatatat aaatagttct cctttctatt aaagatttat ttaatttaca
      781 atctatctac aattatttat taatcactta ttattagata ttcattttcc atatttaaat
      841 ttattctatc cctttatcta tttattttct tactactcta tccaccatta tctatatatt
      901 tattttacga ttactctttc accctaaccc tttaactatc tattttttct tactattctt
      961 atccacatca tccatatatt tattttacga ttactctttc actctaaccc tttaactatc
     1021 tattttttct gatattctta tccaccatca tccatatatt tattttacga ttactctttc
     1081 accctaaccc tttaactatc tatttttttc tggtattctt atctactatt atctgtatat
     1141 ttattttatg attactcttt taacctaccc cctatacttt atagtaatat gtttttaacc
     1201 tttttcatta ttcccaatat gctttataac tacaaatact ctcccccctt aaaaataata
     1261 ataaaattaa tcaataaatt tataatcata ttattactct atgtattaag caattactat
     1321 gccaataaac tttgattttt ttaaacaaat cttaaaaatc ctaaaatgct aattttcttt
     1381 aacttttaat gaatctaata gataacattt aattttcgtt atatttttca gaatatttct
     1441 attattattt ttaagaaaaa aaaataatgt taacatttct taacatacat taaaaaatgt
     1501 taacattata aattgggttt aatactttta ctttgactcg atttactttt attctacttc
     1561 ttactttatt tttaacttag actttatttt tttattactt atttgagtct tatttatttt
     1621 attctcactc catatgttca attacatttg tctaaattct ttgatacttt tattacataa
     1681 ttttaacaag cttaagtgtt atttgggtag tgcagttgat aattatttaa ataatcatca
     1741 actgcatgtt tatatcatta cagtagctta atgtatttaa tcatacttac ttaggatttt
     1801 gaaatatctt agagattata tttaacatta gtcaattgct gcataattgt aagcaattga
     1861 aatttgagac ccatcttcat tttctatagt gataggcttc ataattttta atccttcagt
     1921 ttttatcttt tctaattctt gttccatttt cttaattgtt tcctcaaggt ctttttgact
     1981 tacatctttg actgcattag ggtctttagc atagtctttt aattcttgaa tatgtttagc
     2041 atactcttca taagtataat gctcaacacc atacttttcc aattcagtag ggtcagttat
     2101 tcctgatttt attagagatt caaatttagg taaagcaata ttagatgaat catttccatt
     2161 tgcaaatact gctccagttc ctcctagtac cattgcagat accatcattg cacttataag
     2221 ggattttttt attttcataa ccaatcttcc tttcttagag atttatttgg gtagtgtagt
     2281 ttaaaatgac tattgcaata gttatcaact acactcttag actatcacag tagtcatgtt
     2341 tatgtcaagc ctttttaaaa aacttttcaa taaaaaatac tatctcaaag caaaattaat
     2401 tattttgaga tagtatttta attctaaatc ttttatatta ctaagattta tggttattct
     2461 atccatgttc tatattgttc tttattaact gctaacaaat tcccttttct taatatgaca
     2521 aagcgattat cattatagcc acttcgctca attgaaactt ctcctgaatc agtataaagt
     2581 acatccttac tattactaga tttatttaca tacgtttctt cactattctc agaattagtt
     2641 actttgctca tagatgattc ttcatgctct ctaaattcaa ttacagatac ttttgccgta
     2701 ctaaaatgag tctctccatc acttttagct ttactaacaa gaatatcaga ttctactttg
     2761 ggagaatata aatcagttga tttactactt attgaaaatc caattttatc actttgttca
     2821 ttttttgtta aaccaaaaga ttcaacctca ttaatctctt tattttctgt tgcaaaaaca
     2881 agacctgtcc cacaaactaa tacagttgaa actaaaaaag taacataagt tgatttttta
     2941 aatttcataa tattaactat tctcttttct atttttgtct ttccaaatcc actacatatt
     3001 gtcattgaaa atggattatt ctcctctgct agacttatta gtgtggttgc ataatcagcc
     3061 ctatattctt caccaagtat actcataacc ttttcatcac aactaaattc tatgtcttta
     3121 tttagtaaaa aaaacatcac ccaaactaag ggattaaacc aatggacaac aagagctaca
     3181 agcataaata actttaacag attatcaaag ctattaatat gaaccatttc atgagttaaa
     3241 acaaagtcta aagtttgatt attagcataa ttcatttgct ttggcaaaat aattcgtggt
     3301 tttaaaatac cagatgtaag tggtgtccta gtaaattcag atatgcaaac aataaatctt
     3361 ctaaaacggc tattcttaca catccatttt ttaatgaact catgattttt tatatgaatt
     3421 gaagttttaa aaagattaat tattttaaaa tatctaaagc ataaaagtat gctaaacata
     3481 atagtaacta taaaagacaa aatgcccaac atttccaaca tcataatatt ttcaatatta
     3541 gacattattt caaattcgtc agaattgttt ttacctgtag agtcactagc tttcttaaga
     3601 gtataatttt ctgttgatgt attacccaat tccacatcaa aagtcgattc aattatacta
     3661 taaatactaa aaccagaatt taaacttact ggtattagaa gtttaaataa aacaatcaac
     3721 cacaataata caaatacctt tttaggtaat tttttaaata aacatgctct tataaaaata
     3781 actattaaaa gtaatactcc agaagtaaaa ctcatttcta acaatttaat gaacataaat
     3841 cttacctcac tttaattttt caacaatttg tttaagtttt gtaatctcat cacttgaaag
     3901 attttcttta ttaataaaag ctgaaagaaa tttttctgaa gaccctccaa acattttatt
     3961 aattaaagtt tctgtttcct gtttttgtac ttcctctttg cttattaaag ctttacaaac
     4021 aaaattcggc tcaattctct ctattgcacc ttttgacact aattttttta taacggtata
     4081 cgtagtgttt ctattccatc ctgtttcttc ttttaaaatt tttactaact ctccagcctt
     4141 gctttctcct ctctcccata gaacttccat tactttaagt tctgaatcaa ataattttat
     4201 ctccatataa aatcgctcct caaatatagt tatcacaata gtcattacta taataaaaaa
     4261 ctatcatagt agtctatact atgtcaatca tttatttttt aataataaaa actgtctttt
     4321 taaaaaattc ttataaaaga cagttttatc aatgttatac tttaatgttt aattaaagcc
     4381 atattttatt taaatatata actatctatc ttagtacata gtataagatt tttctttatc
     4441 taatagtata tcctctacat ctgggttttc ttttactaat tgtctataaa aattacaatt
     4501 agtaacaccc acatatggaa ttagccatag tagacctatt ccaagagtag ctattgaaag
     4561 tatataccat cctataaagc taaagtttag tacaaataat ttaagtacat ttcctttcat
     4621 catatctaaa ctagcaccca ttgatttaaa tacacccata ttatccttgt cttcacaaat
     4681 taagaaagta gcttgagaaa catataatag caatatataa actggtaaca taagaacaag
     4741 tattataaga actagagtta ttccagtaga aataatagtc cctattccaa cattatcact
     4801 tgataaatat tcaattgctc cactagtaag taaaactcca aatgataaaa acgctcctat
     4861 aattgtgatt ataaaagaat aaatatacac aataattgat tcaaataaat aaaaaccaaa
     4921 acttcttaaa taaattctcc cacttacaaa gatatctcct atcttgactt tttcatcttt
     4981 agtaattttc aagaacatac atgtaaatcc tgctgaaacg caaaaagcta ttacagccac
     5041 tacaatagta gtcaatggtt caaaaggtaa tcctacaatt gatattaata ctatcaatgc
     5101 aatatatact gccatagaaa ttgcacatat cccccagttt ccagataact gagctttaga
     5161 tactcgtttt aattcttgcc ttgataccat aaaaaacacc cctttcattt tataaaaaaa
     5221 cttttaaaaa tataaataat cttttaacgt ataatattaa tatatcatat tttcgactat
     5281 ataagataca attattcacc atgttttatc aagatatgcc ataattgtat tactagtttt
     5341 atatagtcaa cattaccaaa tacatcaaat aaatttgatt aattattcaa caagtaataa
     5401 aataatttct attaattata ctttatttag taaacaaatt atttaataaa aaaattacct
     5461 aatacagtaa attaattggt aagataaatt atttattatt agtaaagtaa attatttact
     5521 atttatcaag ataaattatt tataaaataa atcatttata aaatataatt tatactttga
     5581 aaataaaata aactatcttc caaacatttt attatatgct taaaagatag ttttgtgtct
     5641 ttaaaataaa ttaaattgta tatattcctt cacataactt ttgtatatta ctaggtggtt
     5701 gccatttacc atctttttta gtatatgtaa tcatagttgt aacttcctta gttggtttct
     5761 tattgtaaag gtctataatc atttttgtca tatcatcttg aacttctttt tcttttttat
     5821 cgtaattctt ttcatcagtt acataagatt ttactaaagt gttcgcttca ctcattactt
     5881 tactagtatc taaagcttta cttacaactt tcaaagttat cttatcctta ctagaagatt
     5941 ccactgttac tgtcttttca acaccttgaa ttaatttaac aattgtttcg tatatctttt
     6001 cagcattttc tttaccataa agaggtgtca attgagcaat cattgaaacc ttgtccttgc
     6061 tgttataagt ttgaatctgt ttatattcag cttctgttaa tgcatcagaa attccttttt
     6121 catcatactt atacatttct aatgcagcta aattagcttc aaaattcgac tttacttttt
     6181 cttctgcatt agaacaccct atagttagaa ctaaaactag agccataatc gtagataata
     6241 ttaacttttt taccattttc atacaaacat cctccctcaa ttaaaataat aaaaaattta
     6301 tttatcaaca ttataacata aatttgtcta actatagaat tattttgaat attttaccac
     6361 ttccagtcta ttccaatacc gttttctttt gctttatcgt atattctttt tgctgttacg
     6421 acatcttgta cacctatccc aacagtttta aatacaatta tctcatcatc atcttctctt
     6481 ccaggaatag ttccgtttat aacatttcct aaatcaccac taaaatcatc tttacttatc
     6541 ttaccttctt ttaatggaat taatatatct cctgactcag ataaaactgc atctgttgat
     6601 tcaaaaaata tcttagacgc tctagttaaa cagtttgggc acaactcttg catatctggc
     6661 atatatgaac ctactgcact tattaaagct ccttttttca ctaagtttcc attaagtact
     6721 ggttgttttg atggagtagc taaaactatc acatctgcgt tatctatggc ttcgtcagat
     6781 gattcaactg caactatttt agtattatat ttacttaatt ttaagttcat ttcttctgca
     6841 aatttttctc tactttcctt gtttctacta aaaactctta cctcatccaa atttctagct
     6901 tctatcatgg cttcaagttg tggctcacct tgtcctccta gacctatcaa agctccaatc
     6961 ttagaatctt ttttagccaa tacgtctata gctgctccag aggctgctcc agtacgaatc
     7021 tgagttacat aagtaccatc caatactgca ctaacttctc cagtttctcc atctaataat
     7081 agtactgttg ctggaattac tggtttgcct ttttgagcat ttttaggaaa tacagataca
     7141 atttttattc ccccacagcc taattcttct acgtatcctg gcataaataa catactagca
     7201 tcttgacttg gaacagatat attggttctc aaaggattta cactctttcc ttcacaataa
     7261 attctaaaag cttccttatc agcttcaatt gcatctctca ttgtaaatac tttttttatg
     7321 tcgtcttttt ttaaaagtag catttttata cccccaattt taataagtct ctatgctaaa
     7381 tattatagca taacatttaa tttaatcttc tatttcaaat caaataaatc tacatactct
     7441 ggttttagca tagaacttat caacgcttta tttattgaaa attctggtat tcctgctgca
     7501 tatggagtta tatcatacaa atcaaaatat acaactagat tctcatcttg caagtaaaag
     7561 ttttgattct cttttatacc tttaaaatta tatattccga tattttcttc atcctttttt
     7621 tcaagttcag ctatttgtct tttaatttct tcatctataa cttctttata tttagtatta
     7681 tccttaaaca agtccattaa ttctaaaaat tttccagttc tcatatccac attatatgct
     7741 atatcttgat aaaatccatg tgctccacca ctataagtat aatatctcac ttttatacta
     7801 agagcagaat cagtattctt tttaagttca aagtctacat ttgcaacaaa cttatttttc
     7861 tcgtcaggat tatccttaag atactgcttt gcttctttat atgatttatt ataaaattca
     7921 aatatgtcat tttttatctt atcatttgta gacctttcaa caattttatt tgaatttgtt
     7981 attacaggca tgtttatagt actttctaaa tactcatcat ttttcactat tgttttactt
     8041 cctattgtac ttactgataa agtttgttta ctatttgtat taccctttgg taatgactgg
     8101 ttactcataa cagtcactgc ttccttatta ctatttaaat taattgaagc atctttcctt
     8161 aaattattat atttatagtc atgtttaatt gccaaaacag ccattatagt caatgttaaa
     8221 acaaatacat atcttaaaac tttagctttt gtatcgactt taaaaaattt catattatca
     8281 ttttcctcct aaatataaat ctctcttatt atatttggag tttctagggt tttttatcca
     8341 tataatttaa taaaagatta atttttattt ataaaaaatt aatctttttg ttttaaaata
     8401 ttaatatgtc ttatcaaaca gtttgaaatt tcaccaagag gtaattgtaa cataacagaa
     8461 cctctatcaa aagctaccat tggcattcca tatactatac aagaattttc atcctgtcca
     8521 attgtaaatg aaccattttc tttcattttt aaaagtccac ttgctccatc ataacccata
     8581 cctgtaagta ttattcctat tgaattttta cctgctactt ctgctactga atcaaacaaa
     8641 acgtctactg atggacagtg accacttatt ctttctcctt tttcacaagt tacataatat
     8701 ccttgtttat ccgtacacaa tttcatctgg taacccccag gtgcaattaa cacatttcct
     8761 tgttctactc tatcattatt cttagcttct cttacattca taatacactg cttgtcaagc
     8821 cgttgtgcat acatataagt aaatatagct ggcatatgtt gaactacaac aatccctggt
     8881 gttgattttg gtaggtcttt tataatttca agtattgcct ctgtaccacc tacagatgcg
     8941 cctatagcaa taaccatatt attaaaacgt tccacattac cgcctccaaa aaatcatata
     9001 tcaaaaatac ctttttatcg tttatatata gcaggtttta catatttgta tttagtacta
     9061 cttctgttga ttacttctgc atgtcctaca aacaaaaaac tatctttttt ggtagcatta
     9121 taaaatttat ttataagttc ttctcttgtt tttaaatcaa aataaatcat aacatttcta
     9181 caaaaaatca aatcaaaatg ttgatatgag aaatcattca ttaaattaaa cggcttaaaa
     9241 attacacttt ttctaatctt atcacaaatt ttaaatgtac catccttgtt gtctataaaa
     9301 tacttcttct tccataactc aggaacattg ttaacatttg attctatgta tatacctctt
     9361 ttagcttttt caagtgagtt agttgaaata tctgtggcta tgattttaat gttccacaac
     9421 tctttattat atccaaaata atcatccaaa gtcatagcta ttgaatacgc ttcttctcca
     9481 ctagaacatc ctgcactcca tatatttatg tttcttattt tattattttc ttcccaaaaa
     9541 ggaagtatac tattctgtat aaactcaaaa tgttgaggtt ctctcataaa aaatgtgtgg
     9601 ttagtactca atctatttat taggtttata gcttcatttc ctgtcctatc tttaaataat
     9661 agattcatat attgactaaa agaactaagt tttttctcta tcattgtatt gtataagcga
     9721 ccttcaatta acgctctttt tttacttaaa tcaataccat attctttttt tacatggttt
     9781 acaagaacta taaattcttc atctgtgagt tttatcatgc aaaaacttcc tcctgctaat
     9841 ttatcagaac taactatatg ctattaatca aaaactttcg aatatctaaa attaatatag
     9901 aattatcttc aactttcgca atctctttta tatctttatt aatattagtg ttgttagaat
     9961 tagtcgtctc tagtaaattt agattttcta tatgaataac atcaacaact ttatctacaa
    10021 taattccaac ttgatactca tcaacctttg taattattat acaagttctc tcattataat
    10081 cagctttagg aagattgtac tttatatgca tattaataac tggtattatt cttcctctca
    10141 aattaataac tccacttata tatgttggaa gacatggtac atacgttatt gattgaaatc
    10201 ttatggtttc aatcacattt tctaattcta ttccataaat aacatcattg acataaaatg
    10261 ttaatacttg tttaataatt tcatctttag ccatacttta aatcccctaa tatatttcta
    10321 taatgctatc tgcatctata attaaactta tacttccatc cccaagtatt gtacatcccg
    10381 ataaaccctt attttttata ttaaatcggt taaaataaac tggaaatggt ttcacaacaa
    10441 cttgctgatt acctattatt tcatcaacaa aaatgcatac tgctttatgc tcattttcaa
    10501 taattatcat attaccattc atcagattgg ttacttttgt ttgaatatta tatattttat
    10561 gtaaccttat aatgggaata catgctccat atattgtaat taactctgtc ttatttcctt
    10621 ctgtaattat ttgatttaag tctgtaagtt taaatgttct tcttatggat gatattggta
    10681 aagtaaatag tgaatctcca actttaaact tcataccctc tactatagaa agagttaatg
    10741 gtatttttat agtaaaggtt gtcccctcat cctgtttact ttttacttca attgaacctc
    10801 caactttttc aatactttta cgaacaatgt ccatgcctac tcctcttcca gaaaattcag
    10861 ttatagattc ttttgtagaa aagcctggag tcataatcaa agaattgacc tcgtcatcag
    10921 tatactcatt actttgatgt ttaagaagac cttgttcctt agcttttgcc aaaatcttat
    10981 ctctcataat gcctctacca tcatctgaaa tacttatcac agcttctccg cctagatttt
    11041 cagcagataa aactatcttt cccacttctt ctttaccttt cgctatacgc tcctcaatag
    11101 gctctattgc atggtccata gcatttctta taaggtgcat aaaaggttcc gctaaattat
    11161 caattacact cttatccacc tctgtttctg ctcctatagt tattaactct acatttttat
    11221 ctaatttttt attcatatct cgtacaattc tatgcatttt ttgaaataca ccagcaattt
    11281 gaatcatacg catagacatt actatatctt gcaattcatc tgtaagcttg tgtaattgtc
    11341 ttgttgcctt attgaaatta tctatatcaa cacctgtaga atttacgcta tttacaacca
    11401 ttgattccat aattacaatt tctccaacta tattttgaag taagtccaat ttttctaaat
    11461 ttacactcat aatactctgc ttacttattg gtaaatgttc attttcttca cttagactat
    11521 ctttttccat atcagttttt tcatttaaaa catcttcttg tatctcatct tctgtatagt
    11581 tattttgata acttttactt actttattat tatcttttaa tttattttct actgcatcat
    11641 tgtttgatgt acttttcatt tctatttctt ttttactaat atttatgtat tcttttacat
    11701 agagattatc ttctatagtt ttataaacta ttgccttatc ctctttagct acaaattgta
    11761 tataaaaccc ataactttta atatactctg tagactctgc atttgtttct acatcttttg
    11821 gataaaaatc tataaattca cagtaatctc taagttcaaa tacaagcatg aaagctctca
    11881 cattttccat catgcaacct tgttcaaaga ttatttttac tacatcagag tcttctttgt
    11941 attcaaaatt tgaatcgatt acttcttttt cgtctttact ttctaaattt gcattatcct
    12001 ttgatttcat agaacttgat aattccaaag tactcacata ctcttcaatt ccttcaataa
    12061 aattagagta atcagaagat atacaacctc ttgattgtaa ctcatctata tctcttctta
    12121 taaaatctaa tgattgaaag actaaatcaa ataataggtc attgtctaaa acttctattt
    12181 taccttcacg tatagcataa aacaagtctt ctacactatg tgctaaagca gatatattgt
    12241 ctaaccccat catagatgct gaacctttta tcgtatgcat tattctaaaa gtttcattaa
    12301 tgttgttatg agtaaatact ttctcttttt ctatttgaat cataatttca tctaattgtt
    12361 cgagtaataa acttgtctca aatataaaca tatctaacat ttgtctcata ctcggttcaa
    12421 tataattcac atttatcacc tacgacctac aaataatttt ctattatata tgcatctcat
    12481 aatttaaatc cttgatgatt ctcttatctc actatatgta cctcataatt taaatacaca
    12541 attattctct tacttcaata tatatacatc tcataattta aatccttgat aattctctta
    12601 tttcaatata tatacatctc ataatttaaa tacatgataa ttctcttact tcaatattca
    12661 cctcataatt taaacccatg atgattctct tacttcactt tcctttagat gaaatttttc
    12721 tactaattct tttaaaattt gagcctgagc agacatctct tctgaggctg ctgcactttc
    12781 ctctgctgtt gctgagttca tttggactac tatggaaatt tgctctattc cagataaaat
    12841 ctgtgtaatt gctacttctt gactatgtac agccgaagaa ataccattaa tataattagt
    12901 agttttttct acaccattta ctaccaagtc caatgcattt gcagtctctt tagcaattct
    12961 gacaccattt tctactgttt taatagcttc ttctatgaga atagctgtat tttgagctgc
    13021 ttttgcactt ttaccagcca aagacctaac ttcatctgct acaactgcaa atcctctacc
    13081 atattgacct gctcttgctg cttcaacagc tgcatttaat gcaagaatat tagtttggaa
    13141 tgctatctca tctattgttt tgattatttt tgatatttca ctcgattttt cacttatttc
    13201 acccattgat gtcatcatat catccataca tctacttcca aaaccaactt gtgatgcaga
    13261 ttggtcagat acctcacatg caagactagc actatctgca cacttcttaa ttccatctga
    13321 tatctcatta atcgtggaag ctaattcttc tactgaacta gcttgttcag tagcaccctg
    13381 tgaaagagct tgtgcacctg ctgcaacttg ttctgaagca cttgcaactt gctctgctga
    13441 taaatttatt tcagaaaaat caccattaaa agagtttatt atactttcta aagatatttt
    13501 aattgattta aaatcaccta taaattcttg gtctatctct actcttaaat ctccttttgc
    13561 aatactagtt aatgtactgt ctataaggtc tatgtaattt aaaagcatac tttgcatctt
    13621 tttgacagaa ctcactaata gccctatttc agatttactt tcttcaacgt ctatacttgt
    13681 tcttaagtcc ccagatgcaa ttcttgaaaa gtgtttttct actttattaa taggaatcac
    13741 tatttttaat tttattactg tatattctat tattgtcaat attattaaaa gggaaagtat
    13801 aaatattgtt cttaatatta gactttgtaa ttcttttttt acttctacaa ttttggaatt
    13861 tgaacgggta actatatcct cttgaaattc atttattaaa gaatccattt tctcttggga
    13921 gtttatatag tcactaccaa aaattatact ttgagcttta ctcttatcat tactttcgac
    13981 tgcctttatt gcactttgtt ctgttggtac taaagaatct gatatttctt ttgcttctaa
    14041 gattttattt gactcgctct ttgtaagacc aattttttct aatttgttta aagctgattc
    14101 tcttgttttt ccgtataatt ctttttcata aatatcttta ttcttttttt ctcctgtaaa
    14161 tacatatctt cttaatacat cacttatctc tctagttgta tcttttatat actgactttg
    14221 agtcctcaaa ttattcatat cattaatact tttttctact ttttcaaatg ctttataaga
    14281 tgaaatagaa gaaaaaatcc ctgctagaac agccaatact ataataatca gcaatacaat
    14341 tgctaattta gaaagcttta cttctctttt catatctgta ctccttcata aagtaagttt
    14401 atatcttttt taatcctttc attgtactca ctacgctaca atcttttcct aagtttttac
    14461 cctcatacat tgctttatct gctaaggata tgttttcttc taatgatata tttgagttta
    14521 cttctactac tcctaatgta atagtgcact ttattttatg ttctttatag ttgaaatcaa
    14581 actcctttat ctctttacgg attctttctg aaagtatctt agcaccttct attggagtat
    14641 ttgtgagcat tatcaaaaac tcttctccac cccatcttga tacagtatca atttgtctac
    14701 aattcttttt tattatactt gatattactg ttaacacgaa atcccctgct tcatgcccat
    14761 aagtatcatt gataacctta aagtcatcaa tatcaccaag gataagggat attgatgttt
    14821 cactatattt attaattctt ctttgctcca taagctgatt aattatatgt cgcctattgt
    14881 aaagacctgt aagataatct cttacaacga gtctattcaa tttttcaaga gaagattcca
    14941 gttcattatt tatctttttt aatttatctt gagatttctt tacttcaaga tgagctataa
    15001 cacgagcttt taattctgtc atactaaaag gtttcaccat atagtcaatt gctcctacac
    15061 caaatccttt tactatgtct gtatctgtat tttttgaagt tataaaaatt ataggtatat
    15121 ctgctgtacg agcattagat tttacttcct tacaaaactc aaaaccagag ttatctggta
    15181 aaattatatc aagcaaaata atgtctggta taaattcttc aataaattct tttgcctcta
    15241 ttgcattaaa tgctttttta accttaaaat tatcttttgt aagtgtttta tctattaagt
    15301 cacacatcag aacactatca tcaacgacta aaattgttcc ctgttgctta tttttcatat
    15361 acaaacctct cttaaacata atatattcaa attttaatga tttaaaattt ttatacttaa
    15421 taattatacc actatattct taaatgttct catcacaatc tttttataat aacactccaa
    15481 ctacatccct atgttacatg atagtgcaat caaacccatt gattaattca tctattaaaa
    15541 cttcatcttt atctgtcaaa agtttctcca aatctagtaa agtaattatt ttaccttcta
    15601 attctccaac tccacttaca aaataatccg aatattctat agtaactctt ggtggaggtg
    15661 atattttact tttttctaag cttactatat catcaactct atcaaccaca aatccaacta
    15721 ccaagtcctt tatttcagtt actactatat acatgcaatt tgtagtcatt aattcatctt
    15781 ttttaagtct caaacgcata tctattatcg gaatgatttt tcctcttaga tttattatgc
    15841 ctttagaata tgtaggtagt tctggaataa atataatttc ttgatttcca gtaatttcta
    15901 ttaccttttc aagttcaacc ccaaaatctt gctcatctac tttaaacttt agatacttaa
    15961 ttccttcatc aattagtcca taatctaaat atgggtcatc tggaaatctc atttaatacc
    16021 tccctcctaa aatacacttt ctatgaaata tatattataa tttcttctta cctttattta
    16081 ttgaatctac aagtaattca cccgtctcag tgtaaaaacg tattgttcta ccataattca
    16141 tacctgtgtc ctcagctaat attggaatat ttaatcctaa aagagttttt tttactgcct
    16201 caacattcct atgtcctata cttgcaatta ctgaattgct acttcctaaa aacatctgag
    16261 cacctccagc aatttttgct tttatatata ttctatttgc acctatattc tccatagatt
    16321 taatgagctc atttattcct gtgtcagcaa atttacactt attttcaatg ttaatagcat
    16381 ctttactata tggcaacata acgtgtaaca ttccaccaat tccagctact ttatctatca
    16441 aacatactcc tacacaagaa ccaagtgcat aagtaactaa aacatttggt ctataggcta
    16501 tcttcatatc tgcaatgttc actactatct ctctattcat acttctaatc ccaacctcac
    16561 gagtatttct ctaagtgagc tcatttctgg aatcatcaaa atgtgactag taaaataatt
    16621 atcactcatt ttaaatttat tttctataaa taagactttg tcaccaacat ctgaaaatct
    16681 tatcataggt acatttagaa cagctccaac catatcaata catatatctg gaattgaaac
    16741 acttatagtc atgtttaaca tagatgcaat agcatttaca tatgaacttg ccataatatt
    16801 tcctatttcc ttaattgctg aaatttcaat tgcctcaatc tcatctaaaa aagcttcttc
    16861 tttattaaaa agagaactta gcataagttg agttattgat tcatccaaca aaaacataaa
    16921 cattccattg atatcacctt tcatatcgag taaaatacat actactttgt tttctggacc
    16981 accaagcaaa tttgtcgctt cattatattc aagtatcttg accataggta ttaatacttc
    17041 tacattgcta tttatcatgg atgcaagtgc agtaattgca ttcccagagc ctatattacc
    17101 aatttctcta agagcatcta tatgaacatc atcaagttct aaataattta taatataaac
    17161 ccctcccttt attattgcat ttatttgctt ttcattgtat tagtgtatat ttacactttt
    17221 attttaaagc attatctaca actttaacta atgtttctgt tttgaagggc ttcactataa
    17281 aatctaacgc tccaagtttt atggcttcta caatcatatt ctcttgtcct attgcagagc
    17341 acattacaac cttagcactt gaatccaact tttttatttc tcttagcacc tctaaaccat
    17401 ctttttttgg cattgtaata tcaagcaaca tcaaatcagg cttttcatat ttaaatatat
    17461 tacacgcttc ttctccatca tttgcttcta ttaaattatt atagccactt ttactcagag
    17521 cttctttaat tatcattctc ataaaaatag catcatctac tatcaaaatt ttattttcca
    17581 taaaactaac tcctccatat gtacaacaat aaacgctttt atcttcagtt attgtctata
    17641 ctatctgact atttttcgct acacaataaa tacatttcat tgataaataa aagtaattat
    17701 ttatgccata ttatacttaa ttctcttaac ataatttgtc aacaaaaaaa cttatatatc
    17761 tattgtaata tatgtttggc gttttgacga gttattaaaa atataattgt ataaaacata
    17821 aaaagttatc aaaagtaatt aaattataat aaaacaactt tatacctttt ttatgtttgt
    17881 ataaaaaaaa ttttcagatt cattaaaatc atttctgtag attcatatag gatttgactt
    17941 tctcaatatc taatagatag ttgtttattt acttaattat atttctctat atgtattatc
    18001 gattaaatta gatttagctt tatacattat tttttatatt tttcaaattg tatactcata
    18061 aatccagaag tattctaatg tgtttataat actttttttc atattttaaa ttaaataaca
    18121 aatcattata tttacaagta tcatttatca ataaacaata ttaaatgaag aatatgtctg
    18181 aagattcaaa gtgatactga tactattaat tactaaaaaa gcagtaaatc ttttacagat
    18241 ttactgctta tagttataaa taataaaact atctataata aatgtactat actcaatatt
    18301 tcattcaata tagtaataac aaatctactt tattcctatg taaatatgaa tttcttgttt
    18361 ctttgtatca aaagtattac attgatactc ttcaaaatca cttatataag tcctatcaaa
    18421 atccatttgc caaaactcaa accaaaaatc tcctacttct ttttgacctc ctactataac
    18481 aaactttgca tacttacctg ctggtataac tttactaata atactttctc caacagtatt
    18541 attagtgttt aattcttcta aattcttctc tttatcagaa ttactattta cttcacaaca
    18601 tgcaacaaag ctatacggac ttgtaaaatc accctggtaa tctgtatata gacctattgt
    18661 cttgtcattc tttttaccct ttatagcatt atatatccct ttgcccataa attttttcca
    18721 caattcacct atatcattta ccgcttgtcc atctttattt gtagtctcct tcgatattcc
    18781 tataactatt ttctcattta tctctactat ttcataattc atactttata cctcactttg
    18841 tttttattaa tctattttat tttgaactaa ttcaattata atttataata tacgacacct
    18901 ttatgtcata tttttaatat aattaaactc agtgagatat tatat
//
LOCUS       DAYAOY010000084        18580 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYAOY010000084 DAYAOY010000000
VERSION     DAYAOY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.084"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..321
                     /locus_tag="ACGI6B_002914"
     CDS             <1..321
                     /locus_tag="ACGI6B_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9752331.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="ACGI6B_002915"
     CDS             337..1920
                     /locus_tag="ACGI6B_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752332.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI6B_002916"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI6B_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFG9752333.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="ACGI6B_002917"
     CDS             3475..4827
                     /locus_tag="ACGI6B_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFG9752334.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="ACGI6B_002918"
     CDS             5019..6128
                     /locus_tag="ACGI6B_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9752335.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI6B_002919"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI6B_002919"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFG9752336.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI6B_002920"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI6B_002920"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HFG9752337.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI6B_002921"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI6B_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFG9752338.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12637..13659
                     /locus_tag="ACGI6B_002922"
     CDS             12637..13659
                     /locus_tag="ACGI6B_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFG9752339.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13719..14726
                     /gene="gap"
                     /locus_tag="ACGI6B_002923"
     CDS             13719..14726
                     /gene="gap"
                     /locus_tag="ACGI6B_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFG9752340.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14948..16150
                     /gene="pgk"
                     /locus_tag="ACGI6B_002924"
     CDS             14948..16150
                     /gene="pgk"
                     /locus_tag="ACGI6B_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFG9752341.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16176..16919
                     /gene="tpiA"
                     /locus_tag="ACGI6B_002925"
     CDS             16176..16919
                     /gene="tpiA"
                     /locus_tag="ACGI6B_002925"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFG9752342.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16931..18460
                     /gene="gpmI"
                     /locus_tag="ACGI6B_002926"
     CDS             16931..18460
                     /gene="gpmI"
                     /locus_tag="ACGI6B_002926"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFG9752343.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLIS
                     K"
BASE COUNT         7426 a         2156 c         3750 g         5248 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaatgaaaa
    12421 tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg atttgaaaat
    12481 ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac tattgaaatc
    12541 tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca ctagggacaa
    12601 aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt agaaattcaa
    12661 aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat tttaagacaa
    12721 atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga gattagtgaa
    12781 agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga cgtagctgta
    12841 tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa ggacatcatg
    12901 agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct tggaataaaa
    12961 agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa agatgtaact
    13021 agatatggat gcgaatattt cttaggcatt ttaaaagatg gagatatcgt ttcaataaca
    13081 ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag atatccaaac
    13141 tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca atctaacaat
    13201 atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca tataccagat
    13261 gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa gacaactttg
    13321 gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga tgtcatggca
    13381 aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa tgcagttgga
    13441 gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact gaacactgtt
    13501 ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt tgcagggact
    13561 gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt tcttgtaaca
    13621 gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc tagcacagag
    13681 ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta gctataaatg
    13741 gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa gataagttcg
    13801 aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta tttaaatatg
    13861 atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct tttgtagtaa
    13921 acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca tgggctgaat
    13981 taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat aaagctgaag
    14041 ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact ggagatttaa
    14101 aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca gttatatcag
    14161 gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat gataaatacg
    14221 gtatagaaaa aggcttaatg actactatac atgcatacac taatgaccaa aatactttag
    14281 atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga aacatagttc
    14341 caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta aaaggaaaat
    14401 tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa ttagtttgta
    14461 cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa gcttcaaacg
    14521 aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga ataagctacg
    14581 gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa caattagtta
    14641 aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata agaactttag
    14701 gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt agattgacaa
    14761 agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa aaatatataa
    14821 aatacaccta aataatgtta catactatat ataaaaatat gataatatga tatatttaat
    14881 agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat taaaaggaga
    14941 gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt gcggaaaaaa
    15001 agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta ctgatgaaaa
    15061 tagattgaac ggagctttac caactattca atatttaatt tctaaagggg caaaagttat
    15121 attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat ctttagcacc
    15181 agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag cagacgataa
    15241 tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag atgtagtatt
    15301 attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt tctctaaaga
    15361 actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc atagagcaca
    15421 ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat acttaattca
    15481 aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat ttacagctat
    15541 acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat tagaaaaagt
    15601 tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc agggttatga
    15661 agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa tgatggaaaa
    15721 agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg cagaccactt
    15781 tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag attacatggg
    15841 actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag aatcaaaaac
    15901 tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa atggtacatt
    15961 atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg gcggtggaga
    16021 tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg tatcaacagg
    16081 tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg cagcattaga
    16141 taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata attgcaggaa
    16201 actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa gttaaagata
    16261 aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta ttaaaggatt
    16321 taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg cactttgaag
    16381 aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata gatatggact
    16441 atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat gaaactgtaa
    16501 acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt gttggagaaa
    16561 ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa gttgaaaaag
    16621 ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat gagccaatct
    16681 gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt atatcttata
    16741 taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga atacaatacg
    16801 gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat atagatggag
    16861 ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt aatttctaag
    16921 gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg gatacaataa
    16981 ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata gaataaaaaa
    17041 agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac ctgatggtca
    17101 aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag tatatcaaga
    17161 tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata aagttttatg
    17221 tgaagcaatg gataatgcaa aagaaaactc tcttcatgta atgggacttt tgtctgatgg
    17281 aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa aagataaagg
    17341 cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc ctcaaagtgc
    17401 attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag gtgaatttgc
    17461 tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa gagtagaact
    17521 tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg aggaagctat
    17581 tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag ttattatgaa
    17641 agatgataaa ccagttggaa gtataaagga aaatgattca attatattct tcaactttag
    17701 accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg atggatttaa
    17761 aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa ctatagagaa
    17821 tgtgcatata gcatttggac ctcaatcttt agccaacact ctaggtgagt atctagctaa
    17881 aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg taactttctt
    17941 cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga taccttctcc
    18001 aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga cagataaagc
    18061 tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg caaatcctga
    18121 tatggtaggt cacacaggaa gtatagaagc ggctataaag gctgttgaaa ctgtagatac
    18181 ttgtgtagga agattgatag ataaaatagt agagttaggt ggaagtgcaa taataactgc
    18241 tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg taacggctca
    18301 ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg caaaattatt
    18361 ggatggagga agattatctg atatagcacc tactatttta gatatgatga agttagaaaa
    18421 gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga taagtagata
    18481 ataatatata aactacaata taaatttaaa aataatgcag tttaggttta ttttgtaaag
    18541 tttagacttt atgacataag gtttaaattt tgtaagttct
//
LOCUS       DAYAOY010000085        18274 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYAOY010000085 DAYAOY010000000
VERSION     DAYAOY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.085"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            129..473
                     /locus_tag="ACGI6B_002927"
     CDS             129..473
                     /locus_tag="ACGI6B_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9752344.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(632..2728)
                     /locus_tag="ACGI6B_002928"
     CDS             complement(632..2728)
                     /locus_tag="ACGI6B_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HFG9752345.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(2741..3901)
                     /locus_tag="ACGI6B_002929"
     CDS             complement(2741..3901)
                     /locus_tag="ACGI6B_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9752346.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(3920..4987)
                     /locus_tag="ACGI6B_002930"
     CDS             complement(3920..4987)
                     /locus_tag="ACGI6B_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFG9752347.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            5339..5905
                     /locus_tag="ACGI6B_002931"
     CDS             5339..5905
                     /locus_tag="ACGI6B_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9752348.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(6119..7105)
                     /locus_tag="ACGI6B_002932"
     CDS             complement(6119..7105)
                     /locus_tag="ACGI6B_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFG9752349.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(7135..7830)
                     /gene="pxpB"
                     /locus_tag="ACGI6B_002933"
     CDS             complement(7135..7830)
                     /gene="pxpB"
                     /locus_tag="ACGI6B_002933"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFG9752350.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(7850..9070)
                     /locus_tag="ACGI6B_002934"
     CDS             complement(7850..9070)
                     /locus_tag="ACGI6B_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFG9752351.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(9099..9890)
                     /gene="pxpA"
                     /locus_tag="ACGI6B_002935"
     CDS             complement(9099..9890)
                     /gene="pxpA"
                     /locus_tag="ACGI6B_002935"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFG9752352.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            10478..11107
                     /locus_tag="ACGI6B_002936"
     CDS             10478..11107
                     /locus_tag="ACGI6B_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HFG9752353.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(11221..12735)
                     /gene="glpK"
                     /locus_tag="ACGI6B_002937"
     CDS             complement(11221..12735)
                     /gene="glpK"
                     /locus_tag="ACGI6B_002937"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFG9752354.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            12979..13521
                     /locus_tag="ACGI6B_002938"
     CDS             12979..13521
                     /locus_tag="ACGI6B_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9752355.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            13526..16027
                     /locus_tag="ACGI6B_002939"
     CDS             13526..16027
                     /locus_tag="ACGI6B_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9752356.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            16448..16846
                     /locus_tag="ACGI6B_002940"
     CDS             16448..16846
                     /locus_tag="ACGI6B_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752357.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(17063..17272)
                     /locus_tag="ACGI6B_002941"
     CDS             complement(17063..17272)
                     /locus_tag="ACGI6B_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752358.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(17304..17513)
                     /locus_tag="ACGI6B_002942"
     CDS             complement(17304..17513)
                     /locus_tag="ACGI6B_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9752359.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            17812..18231
                     /locus_tag="ACGI6B_002943"
     CDS             17812..18231
                     /locus_tag="ACGI6B_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752360.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
BASE COUNT         6288 a         3034 c         2212 g         6740 t
ORIGIN      
        1 agctaaaaag ttatatgttt ttatggatta ttatcagaat atataatata attttatata
       61 gtatagactt tttttaattt attatttaga tttttaatag attttttaac tatggaggta
      121 tttaaaatat ggaaataaag agatatgaag gaactggaag aatgagtaga gctgtagttc
      181 ataacaatac agtttattta tgtggacaaa ctcatgctga aggaggagta attgaacaaa
      241 caactgaagt tttagctaaa atagaagatt tattaaacaa atatggttca gataagcaac
      301 atttgctatc tgttactatt tatttaagag atatgaaaga ctttgaagct atgaactctg
      361 tttgggatgc ttgggttgaa aaaggatttg aacctgctag agcttgtgtt gaagctagac
      421 ttgctagaga gcatttgtta gttgaaatga gcgttgttgc agctacaaaa taatcaattt
      481 agcttatagg aggctatctc aaaacagaga gataagcctc tttttcattg ttgaaatgat
      541 gaaaatacat ttgattttgt ataaaagaaa gtgtctcacg aactaaaaaa ttcattttga
      601 gacacctcct cactatttaa tctatataaa tttacttatt ctctattggc aatatcaatt
      661 tagatggata tttacctcca tgataaatcg tattctttgc ttctatatat tcaatactat
      721 tatatccctc tgtcgtgttg ctattttgga aaataaagtt tttagcacta gacgttatat
      781 ctagtctaat tttatgacca actttaaacg tgtttgatat ttttgaagtt ataatcgtaa
      841 acttataaat ctctccttct tccataaact ctgacttata aaaactattt ctaaatctag
      901 cactaagtat accatcagct aatttaattg aatttccatc taaatccacg tccataattt
      961 ttataaccca gtcagtatct ttggcagaac ttgatgcaaa aaactctaac tttatatcac
     1021 ctgttacagt aaattcttca ctaaaagcat ctgatgtata acacaacata tcacttcttt
     1081 tttctaaatc tttgtagtta tttggaactc caacttcatt ctctgacata tcaattagtt
     1141 gtactgatgg gtcttttgga ttatatatat aactatcata cttttcttct aaaccattat
     1201 caaatactaa tctaccattt cctaaagatg tgttagcatt tccatcactt attagataca
     1261 tacttttatc tatcttattt attattggcc aattttcttc tgtcttccac ttattagaac
     1321 ctacagagta atactctact ggagcagtcg tatctatgtt attttctata ccttttaagt
     1381 atttatcaaa ccataataga taattataat ccaaatcata tcttaatgaa ctatttccta
     1441 aagaaatacc atttatatct cttaaagtat ttgaattatg catccatgga cctagaatag
     1501 cctttctctt tcctttttca tagtccttta taacatctaa ggcctctgta gttcccatac
     1561 tattatcatc ataccaccca gatacaacca atgcaggtac atttatttta tctttttgta
     1621 aatgccaatc catcaaatcc caatactcat ctttttctac tctctttaac cattcttcca
     1681 gaaattcaat tctatatcca agagcttcaa caggtatatt ttctattggt cgtatgttta
     1741 aaacatcatc ccaatcatct cttaccattt tgcttctatc aactttattt cttgatacca
     1801 taaatgccca tgcaagcata ccagatacaa aagctcctcc ttttcttggt atatcaataa
     1861 aagggcttcc tgatgttact atactaacta aagctttaag atgcttattg cctgaagatg
     1921 cagctgccca ttggacataa ccaagatatg atgctccaag cattccaact attccacttg
     1981 accattcctg tgatactatc caatttatgg tactatctcc atcttccctc tcatgaatca
     2041 taggttccca cttaccttct gattcatttc ttcccctaac atcttgtatt acaacagcat
     2101 atcctctttg tacatattta taatatattt ctttatcatt ttcttttcca tatggagttc
     2161 tcataagaat tgtcggtgct tttttggtag aatctacaaa atcagggaga tatacatctg
     2221 tagaaagttt tataccatct ttcatatcaa tcatataatt tccaccaaat cttatagaat
     2281 atatctcttc atttttatga gttttttccc attcttttaa tacagtatac tcttcatatc
     2341 catccataat taatatagta caagtttctc tggataaaca gataaaacct actattttat
     2401 tatctacaat aattatatct agtgggaact ttttgtttct ttgaacccat atatattcat
     2461 taagtttaga atgcgtttct cctaagttat ttataactgt gtctttatct gtgtttacta
     2521 agcttttagt atatacatca aaattattaa ataaagactt taaatctatt tttgtaagat
     2581 ttaaatagta tgtattttct tccttgctta ttggttttaa actttctatt ttatttgtaa
     2641 aaggattcaa cttttcaatt aataaatctc catcaaagat tacatttcca ctgtatagtc
     2701 cagatatgta taatttaaat tttatcaatt aaactccccc ctataatcct agagacatct
     2761 ttattgcata tgccactgat atacctgcat aatttcctaa tactccacca attactccca
     2821 ttacgactgc tacacttaca agcgaattcc actttgcaga agcagctact aaagctgaag
     2881 taggaccatc agcaattgct cctactatag aaagtattac atattgatac tttatcttta
     2941 aaagtctagt tatgcctaaa tggaaaacaa atgaagcaaa aattatgcta aaacagtaaa
     3001 gtgttatagt aaaagttgaa cctaaaaatt cttttatatc tactgaaaaa cctattgtac
     3061 ataaaaagaa taatgcaaca aataatccta aatctaaatt tccctttagt ttctttactg
     3121 gctttaattg agcaattata attgaaattg ttgtaactaa aagtattctt gctgcactac
     3181 taaaactcga attgaaatat ccagcaagta ttgttgcaac ttctgtaaca acaaaaccta
     3241 tagccaacat ccaagctata tctttaatac tccattcttt tgactccata aattcttcat
     3301 tttgacaatc ctctaattct gattcttcta catgatatgg ccaaagtttt ttaaacttct
     3361 ttgaattttt taatattgct ggtagagcaa acataaatat caatgttggt actccaatta
     3421 catagtctgc tgcatttgct gcagcaagag tttgtctact tacattaaga cctgttccaa
     3481 tagctgtaag attagcactt ccaccagtat atgtacctac aaacattcca gcaaccttcc
     3541 aaccttctga tattttttct gcaaatacta atccaaataa aaatgccatg atacttacag
     3601 ttaatacagc tacaaaaatt gcaattaaag gttctcttga taattttgtc atttctttta
     3661 agtcaacact catcaacatt atagtcatag aaactggaat agcatatgtt gaaatagttc
     3721 catataactc agtacttact ggaactactt ttaagtttga aagtattatt cccattatga
     3781 ttattgtaag tgctggtcct aagcttttaa tggctttgta tctttgtaag taaaaaccca
     3841 atgcaatcat tgatgtcatt acagcaagta tactacctgg atttgaaaaa agtgtattca
     3901 tatttaacct ccaaatttat cataagttta cttctattcc taaacctggt ttattgacaa
     3961 gattcataat atctccatcc attacaaatc ctccagatat accttctaat tcttcacaaa
     4021 acataaaact atcaaggtca gcttctgtta tgttcttttt agatgctatt aaatttgctg
     4081 ctgctgttat accaattctc gtttccagca tacatcctaa catacaattt actccacttg
     4141 cctcagctat gttgttgatt tttatagcag gatatattcc acttgatttc atcagcttaa
     4201 tattaaatgt atcaactgca ttctttttta tagccttaat tgcatctatt ggtgaatgta
     4261 atgattcatc tatcataatt tttgtattac ttttatttct tatgtaagca gtaccatcta
     4321 aatcccaatg tggaagtgct tgttcaattg catcaacatt aaattcttct aactttttga
     4381 tagtatttat actgctattt acgttccatc cttgatttgc atcaattcta attcttatac
     4441 tctctccaac ggcttctcta attaacttta cagcttcaat atcgtcttca ggatttatac
     4501 ctgctttaag cttcaatatc ctaaaacctt cttttactct ttctaaagct tctactgcca
     4561 ttttttctgg cttatctata cttatagtaa tatccgtttg gactttacta tcaaatcctc
     4621 caagaacttt ataaaggggt gcattcataa tttttccttt aatatcatat aaagctatgt
     4681 caatagctgc ttttgcagaa gtattaccta taataattcc atccatgatt gtatgtatgg
     4741 tttcaatatc tagtgggtct ttgcctatta actctttttt aaacataagc aatactgaaa
     4801 gaacattatc caaaacttca cctgtaactg ctgcaagagg cgctgcttcg ccaaatccac
     4861 aatacccttc atcagtttca atttttaaaa ttatactttc tccatattca attactccaa
     4921 atgaaactac tacaggtttt tttaatttaa ttctaagctt ttcaaatttg atatctgtta
     4981 tcttcatatt accctcctta ttttttgaag ttataattca atttttaaaa ttttatactt
     5041 taaattatat tattacttca atatatatgt caagataatt tactgaaaat ttactctttt
     5101 ccaaaatata atgtgataat cttaattcaa ttaatactct tctattaaat ttttttatta
     5161 ttattttctt tgtattaatt taacaataat tcattttcat agacttttat ttggatttaa
     5221 ttaaatttta attgtcaaaa tctactatgt attcttattg ccactacttc tatgttgtgc
     5281 tataattact ttgttttaag cgaatatttt atattaattt tattatttgg aggttcttat
     5341 gctaggtgaa aagatgagaa atataagaaa aagcaagaaa aaaacattaa gtgatgtttc
     5401 caaattgaca gatttatcta taagttatat ctctcaaata gaaagagatg ctattgaacc
     5461 atccttgtct tcattgagaa aaatcgctga agttttagac actcctttat atatgtttat
     5521 ggatgacaat aatactgatg atttagtcat tagaaaagaa gatagagtta tgatgaaatt
     5581 tccaaagagt gaaatgtttt acgagatagt atctccaatg cctacaactg agtttacacc
     5641 ttcaatttta tttgtagagt ttgaacttaa accagaaagt gaggatgcca aagattatat
     5701 atatcatgca tcagaagaaa ttatggtggt aacgagtggt gtggttgata tttgtatggg
     5761 tgaaaaggta gttagattaa atccaggtga ctcaacattt ataaaagcta atgtaccaca
     5821 taaaatcatc aatccaagta aagatacaat agctcgtgga tatggagtta tatcacctcc
     5881 aatttggcca ataaaatcta aataaactca atatgacaca cctacttttt gtagataata
     5941 taatagattt aaatattgct tttattttaa aattgaagta tttatttaaa tttaaaatat
     6001 ttactcaaac taaaaaaata gctatttgac taatctaaac catcttaatt atggtaaata
     6061 gtaaatagct atttgtaagt tatattataa actataataa aaattgtatt tgttaaaatt
     6121 aaactgctat atcatttaat acttttgcct ttacaactct tatatttttc atactcttgt
     6181 agatatcttg aatcttatct tcttgctctc tcaatagtct atgtgcttca tatatatcta
     6241 caagttcaaa ttttactaca tcatttggct ttgcttgtgc taacttatac aaatctacac
     6301 ttataacgtt ccctatctta gtatagccac cactagtctg tctatctgat aacattataa
     6361 taggttttcc atgaccaggt acttgtatag ctccataaga aataccctca gatataatat
     6421 ctgctccatt aaggtgcttt atactctctc catctagtct acaacccatt ctatctattt
     6481 cattcgtaac tttgtattta tttgataaaa atttctctac ttcaccgtta gaaaaagcat
     6541 caaattgagg accttttaca attcttagta cgatttctga actataagtt ggaatatatt
     6601 ttgaacttaa ttttttattt attgtaaaat ctttttcctg aataaaagta tttatataat
     6661 cgcctttttt aagtgctcta ccttcaaatc caccaatttt agctcttgta taagttgact
     6721 tacttcccat tgcaagtgga acatctattc caccagctat tgacacatag gctctagtac
     6781 ctgatttcac acctctaaat gataatacat cacctggatt tatttgatat gacctccagt
     6841 tttgaatttc ttttccattt attttagccc ctaaatctcc cccagttact gctatttgcg
     6901 tatattcatc aaaggtaatt gtaggaccta acattaaaac ttctaaagaa gcctcatctt
     6961 cagaatttcc tactaatata tttcctactc tatgtgcata ctcatccatt gctccacatc
     7021 cagatacacc atattgacca tagccaactc tacctctgtc ttgtattaat gtttggaaac
     7081 ctcctaattc aactttgaat cccatatata ccactccctg tactataacg atatttaaat
     7141 acttaagtta tttttttcga tttctataaa ttcatctaat gttattggaa caaattttat
     7201 aaagtctcca gctctaagca aaatctgttc ttctctattt tcatcatata attttaaagg
     7261 tgtcctacca attatttgcc atccaccagg actatcaata ggatatacac cagtctggga
     7321 acctgctatt ccaacactcc caccataaat tttagttctt ggcacttcta atctaggtgt
     7381 agctattctt tcatccatac cacctagata tggaaatcca ggagtaaacc ctagcatata
     7441 tatcaagtac tctctgcttg tgtgtatttt tataacttca tctacagtga gattattgta
     7501 gcttgcaacg tttttaatat ctataccaaa aacctcatca taacatactg gaatctcatg
     7561 tattttaggt tttggtaata ctatactttc caaattatct tctatttttt ttaaattctc
     7621 aatcaaacta tcaaaatcta tttctaatgg attataactt atcataagtg acctatatgt
     7681 tggtaccatt tctgttacaa tacctttaag attcgatatt tctattgctc tcataaatga
     7741 aattactttt ttgtttatat cttctgatat ctcatttcca aattcagcaa ccactgcctt
     7801 atcccctgat atcaagtatc tagtttccat aaatgtcacc tcttctaaat taagcaaata
     7861 aagcacctag gctgctaaga gaagttattc ctgtatatgc agatattaaa actactatta
     7921 atccaaatat taaaagccat gtaggatgct tgtaatctcc aacaatatct tttcttttag
     7981 atgctataag cataattcct aatgtgattg gaagtatcaa tccatttaaa gctcctgcta
     8041 aaattaataa agtagctggt tttcctataa atgccattat aagtgttgat attgctataa
     8101 atgctattat aaaatatttt tcatatttat ctataaaagg atttagtgtc tttaagaaag
     8161 aaactgatgt ataagcagct cctataactg atgtaattgc agcagaccat aatacaagtc
     8221 caaagaattt atatcctatt gctcctgcac taaatttaaa agctgaagct gctggatttt
     8281 ctgggtcaag ttgaagacct tttgaaacca ctgccagtat tgctaaaaat aaaagaactc
     8341 tcatcattgt ggcaactaat atacccaaaa gagaagattt agttatttct tttatatttt
     8401 cttctcctgt tattccaccg tctattaatc tatgtcctcc agcaaaagtt atataacctc
     8461 caacacttcc tccaagtaaa gttattattg ggaatatcag tccttttgga ttttctggtt
     8521 ttaccattct tgatactgcc tctccaacag gaggatgatt tgatattgct acatatccaa
     8581 ctataattat cattcctaat gctaaaaatt tagttatctt atcgactaga gaatttgaat
     8641 tttttgacat aaatacaaat atagctatta atccacttaa tacagttcct aatgtcatat
     8701 tcatattaaa cattacattc ataccaagtg cagctccacc aacatttcct atattaaaag
     8761 ctaatcctcc aagagctact aaaaaagcta ctaaatagcc taatccaggt attattttat
     8821 ttgctatatc ctgacctcta agtcctgata caccaattac tctccataca tttacttgag
     8881 cccctataaa aaggattgtt gtaattaaaa ttacaaatga aaaattcggt ccaaaatctt
     8941 gggtaaattg tgcagtctga gttaaaaacc ctggtccaat tgctgatgtt gccattataa
     9001 atgcagcccc tataagtgct ccgacatctt ttttattttt agttttatct gtaatatttt
     9061 ttgtactcat attttatctc cccctcatgt tatcagtctt atactatgtt ctctgacgaa
     9121 caaacttcta tgttttctaa tggacaaact tctatacctt ctaattcaaa acgctttctt
     9181 atttctttga caaactctat tgcttttgga ttgtccccat gtacacatat agaatctgct
     9241 ttgatatgta taacttcacc tgttaaagtt tctacagttc cttcttttac catcctaaca
     9301 accctatcta ttgcatgttt tgtatcatgt ataactgctc cttctacatt tcttggaacc
     9361 aaaaaaccat tattatcata agctctatct gcaaatactt catttgcaaa cctaagtcca
     9421 atttctttag cagaatttat catttcacta ttatatagcc ccaacagtat aatatcttta
     9481 tcgacttcat atatagcttc acaaatcccc atagctaact ctttatcttt agctgccata
     9541 ttgtataatg ctccatgtgg ttttacatgt tgtatattac atccatttga acttgcaaat
     9601 gccattaaag cacccagttg atattttgta taatctttag cttcctgctt tgttattttc
     9661 atctcacgtc ttccaaatcc tattaaatct aggaaacctg gatgtgcgcc tattttcact
     9721 ccattttttt tagctagctg tactgttttt tccatatgac tagggtcacc tgcatgaaac
     9781 ccacaagcta tattggctga tgaaatatat tttaaaactt cttcatctaa accaatctta
     9841 tacgttccaa aactttctcc taaatcacta tttaaatcta ctttatacaa tttatgaccc
     9901 ctcctcgcta acattttgat agaataagta gttttaattt ttaaattttg tttattttat
     9961 atattgcatg aaatatttta tcacttgtat tcaattttat ttttgcaact attctattgc
    10021 atttaattaa tataatttta ccatttaagg aatatttttg caattatttt aattgaacat
    10081 tcatttgtat ttctttttca aaaaatcttt ttaccatttt ttagttaata attaattatt
    10141 ttcacttaaa ataagggctt taaccttcat ttttaaagta tttttcattt ttaatcactt
    10201 tatatgcaag acagaatcat ttttcttcat tcatttgttt ctttcatcta tataattatc
    10261 attctttgta agtaaaatat ctttatattt ttataattaa ttctttatat agttttaagt
    10321 ataataaaat catgtatact aaatgcatgt taaaatatta ttttaaaaca tgcacaatat
    10381 ttgtacatat tgtagacata taaacctata aattatacaa tagtattgtt aagattatat
    10441 atttttttaa cataaatttt gtattggggg aaaaacaatg gataaaaact atacgatgcc
    10501 aatataccaa aaaatagcgt tggatattgc aaataaaata tacacgggtg aaatacaaga
    10561 ggattcagta ctatttggac gctctgtttt ggctggtaaa tacaatgtat ctccagaaac
    10621 gataagaaga gctgtcaaaa ttttagagga cattggcgtt gttaaaagca taaagggtaa
    10681 aggtgttatt gtactatctc cagacaaagc ttcttctttt ataaaaaaat atagagatat
    10741 tacaaatata tcatcatata aatcaacact ttataattta atagatacta aatctaattt
    10801 agaaaatgaa atattagata ctataaataa aattttagac tattctaaca gacttgaaat
    10861 aatcaatcct ttagtgcccg ttcagttcac cataaattct aattgcaaat atattggtca
    10921 aacagctgca caaactaaat tttggcaaaa tacaggtgct acaatagttg caattaaaag
    10981 aggtgaagaa cttataatat ctcctggacc atatattgaa tttttagaag gagacattct
    11041 cttagtagtt ggtgaccaac acatttacaa ttctatacca atgtttttat atgaaaacga
    11101 aaaataaatt atataattta gtgtgattat ataaaaatgc aagattaaca acaaaaaaag
    11161 agcttcatat tttacataaa gctcttttta aattcaaata aatttaactt tgattaattt
    11221 ttaaatatcc aattcatctt ctttttccca gcttaaagct ctcgatacag cttttttcca
    11281 tcctttatat aatttacatc tcttctcttc actcatattt ggattaaact ctttttctac
    11341 tgaccacttt gaagtaattt catttcttcc aatatagaat ccaacagaca accctgctaa
    11401 ataagcagct cctaaagctg tagtttctac tattttgggt ctatctatat ctacatttaa
    11461 tatatctgat tgaaattgca ttaaaaaatt attattactt gctccaccat caacttttaa
    11521 tgattttagt tttaattttg aatcgttttg cattgcttct aaaacatcct ttgtctgata
    11581 agcaattgat tccaatgtgg ctcttactat atgttctttt tttgctcctc ttgtaagtcc
    11641 taaaattgaa cccctagcat acatatccca atatggagct ccaagacctg taaatgctgg
    11701 cactacatat acaccattag tatcctctac cctatttgca tacatttcac tatcttttgc
    11761 agattcaatt atctttaatt catctctaag ccattgaaca gatgctcctc caataaatat
    11821 acttccttct aatgcatatg ttacttttcc atcaactccc caagcaattg ttgtaagtaa
    11881 cccatgctta gatttaacta tgttactgcc tgtattcata agcaagaaac aacccgttcc
    11941 ataagtattt tttgcagtac cttcttcaac acaattttga ccaaataatg ctgcttgttg
    12001 gtctccagca catcccgcaa taggtatttt agctcctgat aacatgcgct catctgtatg
    12061 accataaaca tagctagaag gttttactaa tggcaacatg cttattggta tgtccaattc
    12121 tcgtaatata tcctcatccc attctaaatc atttatattg tacatcattg ttctagatgc
    12181 atttgagtaa tctgttacat gtacttttcc acgagttaaa ttccatatta accatgtatc
    12241 tatagttcca aataataact ctccttgttc tgctttttcc ctagctcctt ctacattatc
    12301 taatatccat tttacttttg ttgctgaaaa gtatgcatct attaaaagac ctgttttttc
    12361 ttttattgat tcttccaatc ctttattttt aagttcatca catatttctg acgttctcct
    12421 acactgccat actattgcgt tataaatagg tttaccagta tacctactcc aaacaaccgt
    12481 agtctctctt tgattagtta tccctattga agcaatttct tctggtctta tacctgctgt
    12541 ttctataact tctctcatta ccccactttg tgacccccat atttccatag ggtcatgctc
    12601 tacccatcca gcttttggat aaatttgatt gaactctttt tgtgcaattt tcactatttc
    12661 gcctttttta ttaaatagta tagctcttga actggttgtt ccttggtcta atgccattac
    12721 atacttcttt tccatcacca tattctcctt atcatataat atttaattta ttaaaaagaa
    12781 aaatttaata tcacatattt caataaataa tcatacttaa gtacaaattt aatataatag
    12841 atatttagta agaagacact tattttctat catatctata taataatatt atacattata
    12901 gcttacatat tgattaataa attttaaagg agtataataa acagtgttca taataatttt
    12961 aataaaagag gtggtaatat gccaaagatt ttagaaaatg taaaagaaga tatattaaaa
    13021 gtgtctcggg atatgatttt agaagaagat tactcgaata ttagcataag gaaaatagct
    13081 caaaggtgtg gtataagtgc aggtacagtg tataattact ttaattcaaa acaagaaata
    13141 atagaatata tcacaaaatc tgaatgggat ttattgataa ggcgaattga atactccaat
    13201 aaaaacactg aagattacat aaaaaaacta aacattattt ttactgaaat aagaaatttt
    13261 ataaacaaag ttcataatat ccaatacaat gactttttaa acacttttga aacagagaga
    13321 ttttttgaaa tgaaaaaaca caaagacgac tttcatgaac agctttcaaa taaagtatat
    13381 gaagctttag aaaaagaaag tttttttaat aatgacaaat tagtatgcaa tatcataata
    13441 aaaatgtttt tttcatattc aacatatctt catatagaat ttgaagattt aaaaccctat
    13501 attgaaaaat taatacatta aggtggtgtt taccttgaat cataatacta gtattaagaa
    13561 caaggacagt aatccaaaaa tatttttagt acttacgctt tatctattag gtatatttat
    13621 gggagctatt gatactggaa tagtatcacc agcaagaacg attatacaaa atagccttgg
    13681 agtaaatgaa aaaacgggaa tttggatgat taccatatac acattaacat acgcttctgt
    13741 aattcctatc tctggaaaat tagcagataa aataggaaga aaatatgtgt atctagttag
    13801 tatatttcta tttggtagtg gttcattaat atgtggatta tcatcactat tttccaattt
    13861 ctatatttta ttaattggtc gtgttattca agcaattggt ggtggtggaa tcatgccaat
    13921 tgctacagca gaatttggaa ctaccttccc agaaaataaa agaggcatgg ctttaggttt
    13981 agttggggca acatatggta ttgccaatat tttaggttca agcattggta gcacaattct
    14041 aagtatcttt ggaactcaaa attggaagtg gctatttttt gtaaatcttc caatttgttt
    14101 aatcatactt attggtggag ttttctgtat taaaaacaat aagtctgaat ctaaagaaaa
    14161 aatagataag cttggtacat tgatgttgat atgtataatc gtatcactat tatatggtct
    14221 tatgaatata gactttttta attttaaaaa ttctatacaa gatatatcag tttatccata
    14281 cttattgaca tttattgtac ttatacctat tttcatattt attgaaaata gagctaaaga
    14341 ccctatttta agctttgaat attttttaaa tccgagaata ttgatagtac ttattttatc
    14401 tttaattgtt ggtattggaa tgatgggtat ggtattcgtt ccacagtatg ctgaaaatgc
    14461 attaaagata aatgctggtt ctgggggata ttttgtagct atattgggat tatttgcagg
    14521 tattgctgct cctttaagcg gaagacttat tgacaagttt ggggcaaaaa aaatactatt
    14581 actaggattt tctgtatcta tgataggctc tctttaccta gttttaattg ctctaaaaac
    14641 caatactgta tttagtgtat gtgtaagttt aatatttatg ggattaggaa tgggatttac
    14701 tatgggaact cctctaaact atatgatgtt atcaaataca agattagaag aatctaattc
    14761 agcacttgca acattatcct tgatacgctc aattggtact tcaatttctc cagctataat
    14821 gattggattt atagcacatg caggattatc tgtgcaagat aatattatgg acttagttgg
    14881 aaagccctca actcctaaaa tagttcaact tgaagaactt gaaaacatga tagatgactt
    14941 aaaatcagac cctgaaatgg caaaaaattt aaaaaatgtc tctatcccaa atatgaatga
    15001 tacttcaaat ataaatatgg acatgacagg tggcaaactt ccaaaatcct tacttaataa
    15061 agttcagtca gcagatacaa ccaacattac caatataaca aaagaaatat caacaacaat
    15121 gtttgatgaa aaagttccat ctataattac taaaatacag aaaaatgtac aaaaaggaat
    15181 agatggaaca caaagtggaa taaacggtat tgaagatggt gaaaataaac taaatagtgg
    15241 tattaaagga gttcaaactg gtattgacaa tatggtaaaa gctagagctg gtattaatca
    15301 aggaatagat ggtgtaaaaa aaggtattgc tggtgttgat aaaggtataa aaggtatgga
    15361 acaaggtctt gaaaaacaag atgaggccat caaggaactt acaactgctt ataataaaat
    15421 acctactaca gaaaaaactc ctactgataa cgaaaataac tcaaatgaac aaaaccagag
    15481 taataccagt aattctgaat cagacgacac taagaatgat tctacagaaa ataagaataa
    15541 caattccagc gagaataaac caaatgtaaa tgaacaaaaa gaaagtttaa atgctcaaat
    15601 acagcaacta aaaaagacaa gagatgactt aaatacaaag ttacaaaaaa ataaaactca
    15661 aaagaacgaa ttaagtaaaa aactaagaac aatggaatct caaaagaaag agctacaaag
    15721 caagttaaat aattctataa gccaaaagaa agacatggaa aaaagccttg atacaatgca
    15781 acagcaaaaa aaatcactcc aagcagtttt agcaaagaca caagaggtaa aaactgaaat
    15841 accaaaggct tttgataaat caaaattgga ttatataaat tccatagaaa ataatagaac
    15901 aaaaatagaa aacacactac aatctactct aaactcagga tttaaacaga tgtatataac
    15961 agtattctgt gtaaatctac tagcatttat aatattattg ttctataaag aaaataaaac
    16021 tagataaaag agtgttatag tattatattg taaatttatc ttattcttaa atattttatt
    16081 ctaaagaata aattcataaa atttttgtaa ttgattaata taaattacaa tataatacta
    16141 tttttctctt aatctttttc tcttttacag acaatcaaaa agcaatctta gtttatcgaa
    16201 ataaatttat acaatctatt catacacccc tttacattta tataacaatt aaacctattg
    16261 ttatatatac aaatcaaaat aaaccatata ttttcaacta taatatgtta aaatcatatt
    16321 atgagcatat taatttaagc aaaatgtcat ttagacttta atttagtagt tttatattat
    16381 aaatttatat atttttaatt ttaaatgtca tttagacatt actcataaaa agaaaggagt
    16441 gtattttgtg gatactttag gaaaaagaat tgcttatttg agaaactcca aaaaacttac
    16501 tcagcgcaaa cttatggata ttttaaaatt tgaaaactta ggcaagtatg aaacaggaga
    16561 tagaaaacct aattgtgata tactaatgtc aatagctgat tattttaatg ttacgactga
    16621 ctggcttctc tatggtaaag agaaggtaaa tgtcaatagt agtgtaaaag aagataaaga
    16681 agattactta catgttacta atgatgaaat gacgatatta aatctataca gacaactaaa
    16741 cgaacgtgat aaaataaaaa tagaaggaat acttgaactt aagatatctg aatataaaga
    16801 cttaaaaaag cattcctcta ataataatga agataaaatg gtttaaacat acataatttg
    16861 atacgatttt acatcatcta ttaaccttct ttttttgtag tatattacta aaatatggat
    16921 attggattat ttaaaaacaa gtatagtcta atttcaagca catttataat atttagatta
    16981 tcttatccat attttagttt aagtactttg aaaaatataa tacatctcta tgtataactt
    17041 atattcctaa gaaatgcatt actcatgttt ttaattcttc caacatcttt ctatacacaa
    17101 ttgtacatcc ttcataattc ccctctttaa gaaggattga acatgtacta gaatatttag
    17161 ttaaagtatt taatctttcc atatttaagc tttttatatt actgagtgct gtaataaaat
    17221 catcaattgc tctacaacac tctataaccc catctgaatc caatttacac acaagaatat
    17281 tcacctactt tatatactat ttattaatca tcttctgaat attctatatt aagtaattga
    17341 taaattgatg atttatattt tttacctgtt cttcttccat ttaaaatagc tgacaaatag
    17401 ttttcattaa cacctatttt cttcgctaat tccctttgag tcatatttaa ttcaataagt
    17461 ctttgcttga cttgcactcc caaaaaaggt atccattttt ttcgagcatc catttatcaa
    17521 cttcctttct aagtgattta tatttacttt tatattattt tagcttacac accccctcta
    17581 tttagtatat atttttaata tgtacacata ttaagtatat aaatttattt aatactaaat
    17641 aagatttttt gattactttg tgctaaaatt aggatgtaaa ttcgtatcat taagttaatt
    17701 ataaagtcat tttgacctta agtcaatgct ttttatgtca ttttgacctt aaatttgaaa
    17761 ctatttacat cttaaaaagt ctttttgaca ttaattgaat ggagagtgtt tatggatact
    17821 ttaggagaaa gaattgttta tttgagaaaa gcaaaaaacc ttaaacaata tgaactagaa
    17881 gaaatgttgg gctgtgacaa cttaagtaaa tttgagagaa acattagaaa accaaattat
    17941 gaaatattaa aatctatagc tgagatattt aatgtttcag tagactggct attaaatgga
    18001 gataatctat cacataaatc agatttaatt tgcgattcct cctcaaacta tccacttaat
    18061 agcataaact ctaatgaaat aaagctttta aacaatttta gaaagttaaa tgattatgat
    18121 aaagccaaaa tagaaggtat gattgaatta aaacttcatg aatatgaaaa agagaaggat
    18181 ttaggaaaaa ttgagtataa caaaaataaa gatgagaaaa tagataaata atttttatgt
    18241 tgcttttgat acgattttac atcatttctt tgtt
//
LOCUS       DAYAOY010000086        18249 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYAOY010000086 DAYAOY010000000
VERSION     DAYAOY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.086"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            169..1542
                     /locus_tag="ACGI6B_002944"
     CDS             169..1542
                     /locus_tag="ACGI6B_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9752361.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1798..2865
                     /gene="ord"
                     /locus_tag="ACGI6B_002945"
     CDS             1798..2865
                     /gene="ord"
                     /locus_tag="ACGI6B_002945"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HFG9752362.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTIVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3009..3317
                     /gene="ortA"
                     /locus_tag="ACGI6B_002946"
     CDS             3009..3317
                     /gene="ortA"
                     /locus_tag="ACGI6B_002946"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HFG9752363.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3310..4740
                     /gene="ortB"
                     /locus_tag="ACGI6B_002947"
     CDS             3310..4740
                     /gene="ortB"
                     /locus_tag="ACGI6B_002947"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HFG9752364.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4818..5192
                     /locus_tag="ACGI6B_002948"
     CDS             4818..5192
                     /locus_tag="ACGI6B_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HFG9752365.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5186..7390
                     /gene="oraE"
                     /locus_tag="ACGI6B_002949"
     CDS             5186..7390
                     /gene="oraE"
                     /locus_tag="ACGI6B_002949"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HFG9752366.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7390..8799
                     /locus_tag="ACGI6B_002950"
     CDS             7390..8799
                     /locus_tag="ACGI6B_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HFG9752367.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8784..9848
                     /gene="orr"
                     /locus_tag="ACGI6B_002951"
     CDS             8784..9848
                     /gene="orr"
                     /locus_tag="ACGI6B_002951"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HFG9752368.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9878..11299
                     /gene="nhaC"
                     /locus_tag="ACGI6B_002952"
     CDS             9878..11299
                     /gene="nhaC"
                     /locus_tag="ACGI6B_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFG9752369.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11728..12192
                     /locus_tag="ACGI6B_002953"
     CDS             11728..12192
                     /locus_tag="ACGI6B_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9752370.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12426..12788
                     /locus_tag="ACGI6B_002954"
     CDS             12426..12788
                     /locus_tag="ACGI6B_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9752371.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13383..14243
                     /locus_tag="ACGI6B_002955"
     CDS             13383..14243
                     /locus_tag="ACGI6B_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752372.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14501..16615
                     /locus_tag="ACGI6B_002956"
     CDS             14501..16615
                     /locus_tag="ACGI6B_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFG9752373.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            17156..18109
                     /gene="blaCDD"
                     /locus_tag="ACGI6B_002957"
     CDS             17156..18109
                     /gene="blaCDD"
                     /locus_tag="ACGI6B_002957"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFG9752374.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7278 a         1969 c         3369 g         5633 t
ORIGIN      
        1 aaataaaatt ttaaatatac ttaaaaatat attaatataa tgtacataaa aaatatcata
       61 aatatatgat aagataaata caatagcaac attttattaa ttttttgggt ttttatatat
      121 ttaggtactg gggtgatata gttgaaccaa gagatatttt ttaaaataat gaatgaaata
      181 ttggaaaata ttgatgaagg ggtacatttt gttaataaag acgggaaaac tataatctac
      241 aataattctg tacaaaaaat ggaaaaaatg aacagacacg atattttaac caattcattt
      301 tttgatatag tcaacaaaat gaaaataaac aggagtacac tattagaagt tttggaaaaa
      361 agggaagtta tcaaagacaa tattcaaaaa tatctggata aaaatggaag agagataaca
      421 gcaattaata ctacaatacc aattgatatt aaaggcgagt ttgtaggggc acttgagata
      481 tcaaagaata tgacaacgat agaaaattta ctaaacagaa attataaaaa aaatttagtc
      541 aataaagtta gtattagaca aaaaaagggt tatgaatttg atgatatttt aggggaaagt
      601 aatttgatta aagaagcaat agctaaatct aagaaagctt gcaagtctga tgcttcagta
      661 tttatatacg gtgaaactgg aacgggtaaa gagttattta gccagtccat tcactatgga
      721 agtaatagga aaaactatcc atttatagca caaaattgtg cagctttgcc ggaatcacta
      781 ttcgaaggta ttctttttgg aactagcaag ggaggattta caggagcaat agataggcca
      841 ggcttatttg aacaagctaa caaagggaca ttgttattgg atgagataaa ttccatgcct
      901 attatgttac aggctaaact attaagggtt ttacaagaag gttatgtaag aagggttgga
      961 ggaactaagg atatacctat agatgtaaga attattgcaa cgacaaatga aagtccaaag
     1021 gtaattttaa acgagcataa aatgcgaaaa gatttatatt ataggcttaa cataattcat
     1081 ataaaaattc ctcctcttag agatagaaaa gaggacatac ctctattgtg caaaaaattt
     1141 atttcaaagt ataataaaaa gttaaataaa aaagtagtag gaatttcaga tgaagctatt
     1201 aaaattttaa aaaattacga ttggcaaggt aacataagag agctagaaaa cataatttat
     1261 tgtactatga gtatgatgga agatgaggta caaatagaaa caagtatgat taatctaaac
     1321 gactattaca atcttaaaga gaataaagag agtaacaatt attctttaaa cgatttggag
     1381 gacaaaacac ttagtaagat tatcagtgag atagaagaaa agtatatata tgaatcatta
     1441 gaaaaggcat cttacaatat aactaaagct gcatcaattt taggtatgaa taggcaaaat
     1501 ttacagtata aaataaaaaa atataatatc aaaatattat aaataagcaa gataaaaaaa
     1561 caaaatcaaa gtaaaataaa aaagcaaaat agaggagcaa aattattata aaagagcaaa
     1621 atattttgct ctttttttta tttctcataa gaaatattat tttttattta tgaaaataca
     1681 taataaaata attatagttt caacgtttca ataaatattt actttaattt taaagttggc
     1741 acgatttatg ctttaactaa ttattgagaa tatataaatt tttaggaggg tgtatatatg
     1801 agaaaagtaa gagtaggaat atggggattt ggagctatgg gaataggtat ggctaatatg
     1861 attctaaaga aagaaggtat agaaatagta tctgtttgta gtagaagtac ttcaggaaaa
     1921 agtatgtata atgttttagg aattgaaaga ggagaaagac cagaagttat tatcaataaa
     1981 aattatgaag aagtttttag agaaaaaagt gtagatgttg ttttgttagc aactgattca
     2041 tttactaaaa aggcttttga taaaataatt ttcttattaa atagaaaaat aaatgttata
     2101 tcaacagcag aacaaatggc atatccacaa gctgatgatg cagatttagc taaaaaaatg
     2161 gatgaagttg caaaggaaaa tggagtaagt atacttggaa caggaataaa tccaggattt
     2221 gtattagatt tactagtatt ggcgctttca ggcacttgtg aagaagttac tagtataaaa
     2281 gctaagagag taaatgattt atctccattt ggaaagtctg ttatggagga acaaggtgtt
     2341 ggagtaacaa gggaagaatt tataaaaggt gtagaagata agactatagc aggtcatgta
     2401 ggttttgtag aatcaataaa tatgatagct gatggattag gttggaaatt agataagata
     2461 gaacaaacaa aagagccaat aatgactata gtagatagaa aatctaagta cggagaggca
     2521 ttggctggaa atgttgctgg ttgtagacaa tgtggatatg gatatgtaaa tggagaagtt
     2581 ttaattgaaa tggagcatcc acaacagata ataccagaag cagaaggtat taaaacagga
     2641 gactatgtat caataaaagg tataccaaac attgatttac aaataaatcc tgaaatacct
     2701 ggaggagtag gtacttatgc aatgatagta aactctattc cattaataat aaatgctaga
     2761 ccaggtctta aaactatgtt ggatatacca gtacctagag caataatggg agatataaga
     2821 aatcagatag aagttgaatt ggaagaagaa tcaaaggcta attagtaaga taacagctaa
     2881 ctaattagta agatagcaac tagctaatta gtaagataac agttagctaa ttggtaaaat
     2941 aatgtttaat ttaaaataac agtaaattaa ttagtaaggc aatagtcaat taataagggg
     3001 atgaaaaaat ggatgctaaa attaatgatt gggttattat acacaatata gtattgactc
     3061 cagaagaaag agctccacaa gttccagagg atactaagaa agtatcctta gaaatgtggg
     3121 ttaaaggttt tatacaaagt gatgcttcta taggcgactt agtagaagtt aaaacaatta
     3181 caggcagact tgtaaaaggt aatttactta aaataaatcc atattatact catgattatg
     3241 gtaaatgtat accagagtta ctccaaatag gaatccaagc taaagaaata ttatttggag
     3301 gtgtatataa tgaataattc aacatcaatg aaagatatga gttatcaagc tgttatggga
     3361 agaaataacg aaataatgaa aaatgcaatc ggattggatt attcttattt tgaacaagag
     3421 ggaatagggt ttgattatga gaaaatgatg agtgaaacag gatatacatt acaagatatt
     3481 gaagcaattc aatcacagta tgctgttgga aatactcctt taatagagct taaaaatttg
     3541 actaaattag ctagaaaatg tgctaaagaa ggtaaaggag ctagaatatt tgtaaaagat
     3601 gaagcaatga atgcatctgg tagttttaaa gcaagaagag cagctactgc agtatatcat
     3661 gccaaacaaa tgggatataa aggtgtaata gcagctacta gtggtaacta tggtgcagct
     3721 gtagcatcac aagcggcaat gcagggatta aaatgtataa tagttcaaga atgttatgat
     3781 tctaatggtg taggtcaacc tgaaattata gaaaaagcac gaaaatgtga ggcatatgga
     3841 gcagaagttg ttcagttaac tgttggacca gagttatttt atacgttctt agttttatta
     3901 gaagaaacag gctattttaa cgcatcatta tattcaccat ttggaattgc aggtgtagaa
     3961 actttaggtt atgagttagc tatccaattt agagaaaagt ataaaaaaga ccctgatata
     4021 gttgtttgta caaatgcagg tggtggtaat cttactggaa ctgcaagagg attgataaaa
     4081 gctggttcta ttaatactaa agtagtagga gcaagtgtag atttaaaagg attacacatg
     4141 gctagtgata atcaatttaa taaaaaatca tttacaacag gacatacagg ttttgggata
     4201 ccatactgta catggcctga taggtcagat gtacctaggt ctgcagctag accacttaga
     4261 tatatggata gatatgtatt agtaaaacaa ggagaagttt tttatacaac tgaattattg
     4321 gcacagctag aaggtataga aagaggtcca gcaggaaata catctttagc agcagcattc
     4381 tctttagctc aagagttaga tgaggataaa gttatagtgg tacaagaaac agaatataca
     4441 ggcgcaggta aacatatatg tccacagctt acttttgcaa gagaaaatgg aatagatata
     4501 aaatttggaa atccaagaga agaaattgca ggagtaaact taatattacc agaaagacca
     4561 gaattattga aatgtgttga tattgatatg aataaaataa gaaagtcatt tataaaaaat
     4621 tgtatatcta ataatcacat tgataatatt gataagctaa gtaatcgaga tatagaattt
     4681 ttaatggaag aagtaaaaag ttcaagagat tttgttgttg atgtattaaa taatttatag
     4741 taattattgt tttttaattt aatataaaat aaaaattcag aaaattcgat taaatcaata
     4801 cgaatggagg aatgttcatg aaaaaaagag aggatgattt tgaagttaga agaaaacatc
     4861 ttcaagaact gagtgatgat gagttaaaag aaaggttttg gagtttagct acccagatag
     4921 tagaaccaat gatagaactt ggtaaaaaga atacaacacc atcaatagaa aggtctgtct
     4981 tgttgagaat gggattttct tctcttgaag taaaacctat tttagaaggg gttatggaaa
     5041 gaggtcttat aggtaaagga gcagggcatg tagtttataa attggctaaa tctaaaaata
     5101 taacggttag agaagcaggt ttgttattag ttaagggtga atattgggat gaagttacat
     5161 gtttatttag agaaggggta gaatcatgtt aaaggaaaat aaaaaattgg atatagacta
     5221 tatattaaaa gatttggata aatataaacc aaaacgaaga ggttgggttt ggagagaaca
     5281 cttggaaaat cttgaaatgg gaccttttaa atataaagac tgtacaaaac ctttaaagaa
     5341 gagtgtaggg ctaccatcat ctaagtattt caataatata gaccctcaac caagtccagt
     5401 tattactact gaaatagctt ctggtagatt tgaggatgat ataagaagaa tgagaatggc
     5461 agctcatcat ggagctgacc atctaatggt tatcagaact gctggacaat cacattttga
     5521 tgggcttata gaaggaactc cacaaggtat aggtggagtt ccaataacaa gaaaacaagt
     5581 aagagctcaa aggaaggctc ttgattttat agaagaagaa gttggtagac ctattaatta
     5641 ccattcatat gtaagtggtg ttgctggtcc tgaagtagca gtaatgtttg ctgaggaagg
     5701 agtcaatgga gcacatcaag acccacaata taatgttctg tataggaata taaacatggt
     5761 aaggtctttt gtagatgctt gtgaggcaaa gaaaataatg gcttttgcca atatagctca
     5821 aattgatgga gcacataatg ctaatgctac tgctagagaa gcatggaagg taatgcctga
     5881 acttatggtt caacacgctt taaactctat attttcagaa aaaataggta tagataaaag
     5941 taatatatgc ttatcaacag ttccaccaac agcacctcca gcaccatgtt taaaaataga
     6001 cttaccttac gctgttgcac ttagagagtt atttagtgat tataagatga gagctcaaat
     6061 gaatactaaa tatatggaat catcaactag agaagcaaca gttactcatg tattaaactt
     6121 attaacatca gtattaacta gagctgacat acaatcaact ataacacctg atgaaggtag
     6181 aaatgtacct tggcatatat acaatataga agcatgtgat acagcaaagc aagctttagt
     6241 tggtatggat gggcttatgg atatgataga acttaaagat gtgggtgaat taagagacaa
     6301 agcaagagaa cttaaagaaa gagctgtatt atatatggaa gaaatatctg aagttggtgg
     6361 atattttgaa tctgtggaac aaggattttt tgttgattct ggaaattatc cagaaagaaa
     6421 tggtgatgga atatctagaa aaataaaagg tggagttggt gaaggtactg tttatgaaag
     6481 agaagaagat tatttagcac ctgtaactgc tcactttgga tacaataatg tagctcaata
     6541 tgatgaaaat gctatagata atccatcaat gttaatagat ggatgtacat ttgaaaatcc
     6601 agacaaaata atatatattg atgagcttga cgattttgat aatgtagaaa atagactaaa
     6661 agaatcatat gagtacagaa atggaactaa aattaagcct gaaatggaat ggtgtgcaga
     6721 tggaattgta atgataacaa tgatgcttcc tactgataaa agaacagcag agtttgcagc
     6781 tttggaattt gtcaaaaaaa tgaacttaca agaaattgag gtcataagta gagaagttat
     6841 gcatgaatct gaagggacta gaatagaagt gaaaggaaga gttccgtttg atatagattt
     6901 aaacaattta gtaataccag aagaacctaa ggtactaact gatgaagaaa taagagcaga
     6961 tatagaagaa aaacctatga aaatagtttc agctacggtt ggagaagatg aacattcagt
     7021 tggtttaaga gaaataatag atataaaaca tggaggtatt gaaaaatatg gcatagaatg
     7081 ccactatcta gggacttctg tacctgtaga aaaattggta gatgcagcga ttgaactgaa
     7141 tgcagatgca atacttgcat ctacaataat aagtcatgat gatattcact ataaaaacat
     7201 gaaaaaatta cacgattatt gtgtagaaaa aggtataaga gataaagtaa tgattgcgtg
     7261 tggaggaaca caagtaacac ctgaaatagc tgttgaacaa ggcatagatg ctggatttgg
     7321 aagaaacagt aaaggcatac atgtagccac tttcttagtt gaaaaaagaa gagaaatgag
     7381 taataaataa tggaaaatag caaggtttct ttaaaaatag atgtgttggt agctgaaatt
     7441 ggaagtacta ctacagtggt aaatgctttc cacgatatta atactaacaa tcctatattt
     7501 ttgggtcaag gtcaagctcc aacaacagtg tttgaaggtg gcgatgtaag aaatggctta
     7561 tcaggtgcta ttaaagattt agctaataaa ttatctgtag atgatataga gtataatgat
     7621 atgtttgcaa cttctagtgc agctggtgga ttaaaaatga ctgttcatgg attagtctat
     7681 gatatgacag taaaagcagc taaagaagca gctttagggg ctggagctgt tattcgtcaa
     7741 ataacatcag gtagaattaa aagaactgat ttaaataaaa taaaagaaat aaacccaaac
     7801 ataattttaa tagctggtgg agtcgattat ggagaaagag atacagctat ttataatgca
     7861 gaaatgatag cttctatgaa tctaggcata ccagttatat atgctggtaa tgttgaaaat
     7921 caagaggaaa taaggttaat atttgaagat actaattata agctttatat aactgaaaat
     7981 gtatacccta aaatagattt attgaatata gaacctacaa gaagaatcat tcaaagtgta
     8041 tttgaagaac acataactac agcaccagga atgaaataca ttaaagaaat ggttaatgaa
     8101 aatattactc caaccccagg agctgtaatg gaagcttcta aacttttata taaaaatatt
     8161 ggagatttat taactctgga tgtaggtgga gcaactactg atgtacattc tgttacagat
     8221 ggaagtgact atattaataa aattttggtt aatcctgaac ctactgcaaa gagaagtgtt
     8281 gagggggatt taggtgtata tgtaaatatg aagaatatag tagaagttat aggtaaagaa
     8341 aacttacaaa gtgaactatc tatagatatt gatgcagtaa tagagaatta tcctccaata
     8401 ccaaaatcaa aagaagaaat attatttgtt gagaggttga caaaagaagc tgttgttaaa
     8461 gctatgctaa gacattcagg aaaaataaga aatatctatg gaacaactgg aaaagtcaaa
     8521 attgcagaag ggaaagattt aacagaagta agatatatag ttggtacagg gggagcactt
     8581 actaggctcc ctagtagaat tgaaatatta gataatatgc tcaaatacaa taaaaataat
     8641 gaattactat ttccaaagga aaaaacaaag atattaatag ataatgacta tattatggca
     8701 tctatgggtg ttttatctaa aaaatatgaa gaagcatctc ttaagctcct gttaaaaagt
     8761 ttgaattttg aggaggaaag attatgtatc ctaggttaga aatagatata gaaaagttaa
     8821 agcataatgc aaaattaatt tcgcagatgt gtcatgaaag aaatattaag atatcttttg
     8881 ttactaaatc attttgtgca caaaaagaaa tagtagaaga aatatgtagt gaaggaatag
     8941 accatatagc agattcaaga atacaaaact taaaaaattt acaagatata aatctaccta
     9001 agatattgat tagaatccca atgttaagtg aattagaaga agttatcaat tattgcgata
     9061 ttagttttaa ctcagaatta ggaacaataa agaaattaaa tgaattatgt gaaagtaaaa
     9121 atattattca taagatagtt cttatgtttg acttaggcga tttacgagaa ggatactttt
     9181 atgaagaaga cttttttagt aatgtaagag agatagtaag attaaaaaat attgaaataa
     9241 ttggtatagc tacaaactta acctgttatg gagctattat accatctagg gaaaatatag
     9301 gcagactggt aagtatagca aaaagaatgg aagaaaaatt tggaataaat ctagaaatag
     9361 tatctggagg taattcaagt tctattcatc tgttgataaa taataatatg ccagaaggta
     9421 ttactaattt gagaataggc gagtccattc tacttggtag agaaacggca tacggtgaaa
     9481 atataaatgg aacatatcaa gatgcattta aattaatatg ccaagtagtt gaatgtaaag
     9541 aaaaaccatc agttcctatt ggagaaatag gagttgatgc ctttagaaat aaacctgttt
     9601 atgaagataa gggaatctta aaaagggcaa tcatagctat aggtaaacaa gatataaata
     9661 ttgactcttt aattccaata gacactgata taaaaatatt aggggcaagt tcagaccaca
     9721 tgatactaga tgtaagtaac acaaaatatg attataaatt aggtgacaat ttagaatttt
     9781 tattaactta tgggggtatc atgtcatcta gtacgagcaa atatgtttca aaaaaaatta
     9841 tagtgtagtg tttaaataat taggaggaaa ctattttatg aaagagcgag aatgtagaaa
     9901 agcaagtctg tttgatgcaa tgataccaat tacttgtttg atattatttt taagtctagg
     9961 agtattagtg tatggttcat cacctcatgt accactaata ggagcagcag cagtagcagg
    10021 acttgtggct gtatatagac ttggatttaa atggaaagag ttagaattaa gtatgtttaa
    10081 tagtataaag atggcaatgc aagcaatctt aatcataatt ataataggcg tacttatagg
    10141 aacttggata gtaagtggag tagttccatg tatgatttac tggggactaa aaattttatc
    10201 accaaatatt tttttagtag catcaacatt agtctgtgca atagtatctc ttgcgacagg
    10261 ttcatcttgg agcacaatgg gtacagtagg agtagctctt ttaggcattg gtcaaagttt
    10321 aggtatgcca attggattaa tcgtaggttc tataatatct ggagcttact ttggagataa
    10381 actatcacct ttatctgaaa cgactaactt agctccagca atggcaggaa cagatttgtt
    10441 tacacatata aaatatatgc tatattcgac tataccatca ttattaattt gtttagtaat
    10501 ttatggtgta attggaatga agtattcagg tcaagcacta gatataaaac aaatagaatt
    10561 aattaggtct acattagatt ctacatttaa tactttatca ccagtattat tattagctcc
    10621 agcaatagta attggattgg ttgtatttaa agttcctgct ataccagggc ttatagtagg
    10681 tgctgtattg ggagtattgt ttgcagtagt ttttcaagga gaatctatga atacgatatt
    10741 agatgcagca caaaatggat atgtttcgtc aacacaaata caggaggtag atgctttatt
    10801 atctaaaggt gggattatga atatgatgtc cactgtatcc ttaacaatat gcgctttatc
    10861 tcttggaggt attttagaaa aaacaggtat gcttgaagta gtagcatctt ctttactaag
    10921 attagcaaag ggtgtatttg gaacagtatt atgtactatg gtaacttgta caattaccaa
    10981 tataatagca ggtgaacaat atctatctat agttatacca ggaagaatgt ataataagga
    11041 atataaaaag agaggtatac atcctaagat gttgtcgaga gctttagaag atagtggaac
    11101 tttaacctct ccattagtac catggaatac atgcggggcg tatataacag caacattagg
    11161 tgtatctgca ctaacatatg gtccatacgc tttattaaat attataaatc caatagtatc
    11221 tttagtatta attgcatgta agtttaaaat agcaagaata gaagatgaac ctgaaacttg
    11281 tttaaatttt gatgtttgat aatagataac ttatgtaaga acgtaggaac taggggggta
    11341 gcctgtgggg aattagtaag acatatttta gtgtgcgaaa ttctataaat cagtaatctt
    11401 gtgactttag ccatgggagg ttcagattag taaatttata gttttatatt agtctatatt
    11461 ttgatctttt aattgtttgt ataaattttt tatagaaaaa gatttctttt agacttgcct
    11521 aaaaattagg caagtctttt aagtatgttc tgattataag agcactgtgt gacaagaatg
    11581 atgagattat ttttgctata ctttcgatat aagaaatgga aggaggttat gttttatatt
    11641 catatgaaat aattagttac acagtaaatt ttcgtataat ttggtataaa aagattagaa
    11701 atgaaaataa agtaatggag gaataaaatg actagaatat ccaatataac aatagctgaa
    11761 caatctgagt actgtttttt ggcaatacga aaaacaattg actttatggt agagttttca
    11821 gagttttcaa aacaaagttt tgaaaaaatt tctaaatatc tggaggagag aggaatttta
    11881 tcttcaggag caccgattgt atgttttcat aacatggatt tagcaaaatt ggatgttgaa
    11941 gtagggtttc ctgtagccac ttatatagat gggaaaaatg agattctaca aaggattgtt
    12001 cctgcacaga aaataataac tgcaattgat ttaggacctt atgaattaca agaccctact
    12061 ttagaagact tattttcatg ggcaaatagc aatggatata aacttcatgg cgatatatat
    12121 taccagtatt taaatgatat caatcgtcca gctagtgaat atcttacgaa aatgatgtta
    12181 cccattatat agaaagttaa agattatcgt ttgttagttg ataattagaa gaacatttac
    12241 tgctactaca taaattttat cctaaataat atcaaggtga tttaatttat gtattctaat
    12301 gtaaccccaa tatattgtta aggtctactt aagatgctca aaaggctatg cttaatttat
    12361 tgttatataa tttgtagagt ggtataattt agaggtaata ttatatagat atataggggg
    12421 aaaatatgaa aatatatgat atattaacac gtttttatat tcaagatata gaaaaagcca
    12481 taccttttta tgaaaatctt ttaaaagaaa aatgttcttt gcgtttttcc tataaagaag
    12541 ttgggttgga attagcgcaa ataggaaatg ttttactatt atctggttct gatgatgctc
    12601 ttaaaccatt tatagaaaca aaatctactt ttatggttga ttctgttgat gaatggagaa
    12661 gttatctatt ggataatgga gcagttgtag taagagataa gaaaaaagtt cctacaggat
    12721 ataatatgac attgagacat ccagatggta caattataga atatgttcaa catacaaagc
    12781 aggattagtt aaaaacagat tttactactg agcaactaat tagaatctat tcttagacgt
    12841 acaatttatt atttttggta ttaacagatt ataagtcaaa ttataatatt tcaaaataga
    12901 atagaaattt atttacataa aagagagtgt ttaaaaatga actttttagt tcatgaagca
    12961 ctcttttttg tatgaaatca aatacatcaa tatataaaat aaactgatga agagcttaaa
    13021 ttaaaataaa aagattcata acaggacccc attaaacttt aagatagggt aagtttgtaa
    13081 tcatattaaa taattgtaaa aagctgtttt tagtcgtata atataaatat ttatcattta
    13141 attaaggaat ataaaaggaa tctaataaga agaaaataat aaataacttt ttagatttac
    13201 taaatgaaaa gttattgaaa attaaaggat tagagtcttt aatcagaaag taaaaagtaa
    13261 tatattaaat ttatgaattg aaaacttaaa caacaaattt gtttataaaa tagcaagatt
    13321 cattaaatat aactaagtaa taattgatat aatgttgcat ggataaaagg agggaagctg
    13381 caatgtctta caaagtaatt aatgtcataa ctgctattga ctatatagag gagcatttat
    13441 cagaaaagct ggatttagat atagttgcta atgctgttca ttactcaaaa tatcacctgc
    13501 atagaacttt cacaacttct gttggtctaa caatgcatga ttacataaag cggagaaagt
    13561 tgacagaagc agcaaaatta ttggtatttt caaagaaacc aattattgaa attgcattga
    13621 tagctggata tgaaagtcaa caggcattca cttctatatt taaagctatg tataaaaaat
    13681 ctccaaataa atataggaaa gaacaagaat tttatccttt gcagctaaga tttgttttga
    13741 aaaaagataa cctattttca aagaatgtac tagagttgga aaaagaaatt aaacttgcaa
    13801 gtatgtctga cataccatta tggatgaatt tagtgagatt agtcatagat ggatttccaa
    13861 atttgcaaga ggaagaatat atatatcaat tagaacagta tattttagaa gaaagagctt
    13921 taatattaaa acttaataat gtcgctattg caaatatggt gtttaataga gaaactagaa
    13981 gtattgattt ttttggaata catccacagt atcgtaattc agacattgca caagtgtttt
    14041 tgaagaaagt tattgaagat ttcttgatag atacagatat ttcaattact acctttcgag
    14101 agggtgacaa agctgataca ggacatagag atatgataaa gaagttgggc tttgcagaag
    14161 cagaattgct tgtggaattt ggatatccta ctcaaaaatt tattcttcct tgtgtagaag
    14221 aaagagataa tagacaaaat taattgtaga ttaaatgaaa agtttaccac atattttttg
    14281 tttgaattat tttatatata agagcatttc tgcttttaat gtattttgct tgtaagtgta
    14341 cttatggtaa taaatataga aagatttagt tttttaaaaa aggaacttta aagctaaaaa
    14401 gctaacattt aagttaaata gcaagaatga ttaaaaactc caaaacacaa ttaagtataa
    14461 tgattcagtg taagtactta attaacaaag gaggatttaa atgtctgaaa ataaacaagt
    14521 agtaaattgc gatattattg catctaattc taaacataat atgtgtgaat atgatgcaga
    14581 gttattaact ttttttccag atgagattgc acatctaata gagataaata acaagctaga
    14641 caatgctttt aagaaagctg aaaatcttgt tgacaagctt gataaggatt atatggacgc
    14701 taaaatgtat atggtaaaaa atcgtggtga aatagaccca catgaaatgt ttcaaaatga
    14761 acaaggacta aaacaaatag ataactatgg ggcttttatg gtaaaagttc gagataagat
    14821 taataaaatc aaagattctc catactttgc tagaatagat tttaggctga aagatatgga
    14881 tgatgaatct aagtattata ttggtagatt tgcatttgat tatgaagatg agcttataat
    14941 acttgactgg cgttctccaa ttgccagtat gttttatgat tatgaaatcg gaaaagctgg
    15001 atatgatgct ccaataggat gggtggatgg agaaataaca agaaaacgac aatttaaaat
    15061 taaaaatgga aaattagaat atgcactaga aagttccatt aatatacaag atgatattct
    15121 tcaaaaagaa cttagtcaca cttcagatga aaaaatgaag tcaatcattt caacaataca
    15181 aaaggaacaa aatcaaatta ttagaaatga taaagctgat acactgatta ttcaaggggt
    15241 agctggttct gggaaaacat ctatagcatt acacagaatt gcttttcttc tttatcgatt
    15301 taaagataaa atatcagcaa ataatgtaat tatcctatca cctaataaag tctttggaga
    15361 ctatatttcc aatgtccttc ctgagcttgg agaagagcca ttatgtgaat taagttttga
    15421 aaatatagca gaggtacaat tagatagagt tattaatttt gaaagtgaga aagacccatt
    15481 agaaataaat gatgctaaat ggtctgaaag agtgcgtttt aaatctacac ttgattttgt
    15541 aaaacttatt gatgattata taaaacaaat gccaaataag atttttattc caaaagacta
    15601 cacttttgga agttttacag ctaaaagtga ctggatacag agtagatttg aggcatataa
    15661 cagatatcca gttaagaaga gattagagaa ggtagctgaa gacattcatt ataaatttga
    15721 atcagataat attatggaag aagatttacc aaaggtaaaa agtatactta aaagtttgaa
    15781 tggaatgttg acaataaaaa atactcttac actatataag gatttcttta aacaaatgaa
    15841 tatatcaaac atgtttgtga tggcaacaaa aaaaactctt gaatggtcag atgtataccc
    15901 atttatatat attcatgctg catatgaagg tatacaagaa gataagatta taagacatgt
    15961 tgttattgat gaaatgcagg attacacacc tattcaatat gctgtaataa acttactttt
    16021 taagtgtaaa aagactattc ttggagattt tggacaattg gttaatccta atcacactca
    16081 tacactagat gatatgaaac agctatataa tgatggggaa ctagttatgt tgaataagag
    16141 ttaccgttct acttttgaga ttattaattt cgccaaaaag attcaagaca tatcttcatt
    16201 agagcctatt gaacgacatg gtgaagaacc agctttatta aaatgtaata acgagcaaga
    16261 tgaaataaat aagataaaaa tagaaattga agaatttaaa aagagtgata atgcgactct
    16321 tggtattata ttaaagacag ataatgacgc taaagctgta tatgatgcgt taagagaaaa
    16381 atatagtgtt cacttaatat cttctgaaag ttctagcttt acaaaaggag tatccataac
    16441 ttctattaaa atgtcaaaag gtttagagtt tgatgaagtt attgtacctt cagtaaataa
    16501 taaaacatat tatagtgatt atgaccgcag tttgttatat attgcttgta cacgagctat
    16561 gcataaactt aagttgacat atactggtga attgactcaa cttatagata tgtagattat
    16621 atacttatta tgtaagtgtt tacttatata aatataacct gtcaataaaa ttcattaata
    16681 aatcaataaa aacaatattt gtaaaagtaa aagctataaa ttatatatta aattttataa
    16741 tttatagctt ttataatact gcttatctta atcctcccaa tagcaaaata attttaaaca
    16801 tgccatattt tataaatgta ataacaatta tacaattaaa ataactttcc caaactaact
    16861 tgacatttaa aacttactat tgtaatattt aaatattaca atagtaagtt ttaaatgtgg
    16921 aatcaattat tatatcacaa attaaaagga ggacaggatg ttattaatag ttacttaatt
    16981 atcttcgcaa taggtttaat caacataatt gtactaggtg aagaaaatat aagagataca
    17041 gctattcatt tagtcttgtt tcttataagt agaacctaga ttggagtata ttacaaataa
    17101 tgtctactat tttaatagta ggttataaat ttaaatatgg gagatgatat aaattatgaa
    17161 aagaaaaaag aattttattt ggatagctat cttattggtt ggtgtagtta tggtaatgta
    17221 ttatgtaggg aaaaaacata acgatataaa tcaaaaaact gataagaatt atataaaatc
    17281 tgaattaaat aagtctgaat taaataaatc caaaattgaa aacaataata aaaacaaaaa
    17341 agaaaaaatt aatatggttg actatagtga ttgttttgaa ggtattagtg gaggagctat
    17401 tttttataat actaaaaata aagaatataa tatatataac aaagaattaa ttgaaacaag
    17461 aaggtcacca tgttctactt ttaaaatagt ttcaacttta attggtttag aaaaaggggt
    17521 aataaactca aaagaatcag ttatgggata tgatggtaca gagtatccaa ataaaaattg
    17581 gaataaaaat cttagtttag aagaggcatt taaggagtct tgtgtttggt actacaaaaa
    17641 attgatagat aaagttgatg ctaaatctgt ccaaaacatt ttagatgatt taaagtatgg
    17701 gaattgcgat attagcgaat gggaaggaga tttaaaaaat ggaaagggac atttaaatgg
    17761 attttggtta gaatcttcat tacaaatatc tccaaaagaa caagtgcaaa caatggcaaa
    17821 aatatttgag ggagatacaa actttaaaaa agagcatatt aatattttaa gagatattat
    17881 gaaaatagat gtaaatgata aaaacataaa tgtatatgga aaaactggaa caggatttga
    17941 tgaaaaaaat aaacgtgtgg atgcatggtt tgttggtatg ctagagcgtg aaggtgacac
    18001 ctattatttt gctattaaat cagatgattc caataaagaa attacagggc caaaggtaaa
    18061 ggaaattgca attaatataa ttaagaaata ttatagtgta agggaataaa cttaatttta
    18121 tttgtaaata ttatttacct atagtctgta atatttatta aataatttta ctaaattgag
    18181 tctatataga gcaaaactcc tttagtacat aaaatacttg ctattgatta ttaaattgaa
    18241 tacctatag
//
LOCUS       DAYAOY010000087        18242 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYAOY010000087 DAYAOY010000000
VERSION     DAYAOY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.087"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(569..892)
                     /locus_tag="ACGI6B_002958"
     CDS             complement(569..892)
                     /locus_tag="ACGI6B_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9752375.1"
                     /translation="MKKIKINLTHTKSNLAKKYIEVQKKNMLIHTKNKTLDAICILEK
                     IEKDLNIEELVRCHKSYITNLNYVENIKSNTAILDSQEEVPISRYRYKDIKEGFLKFI
                     GDRIC"
     gene            complement(1131..1283)
                     /locus_tag="ACGI6B_002959"
     CDS             complement(1131..1283)
                     /locus_tag="ACGI6B_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752376.1"
                     /translation="MKNLNKNTILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            complement(1793..3322)
                     /locus_tag="ACGI6B_002960"
     CDS             complement(1793..3322)
                     /locus_tag="ACGI6B_002960"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase domain-containing protein"
                     /protein_id="HFG9752377.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
     gene            complement(4021..5451)
                     /gene="dcm"
                     /locus_tag="ACGI6B_002961"
     CDS             complement(4021..5451)
                     /gene="dcm"
                     /locus_tag="ACGI6B_002961"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFG9752378.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            5664..6380
                     /locus_tag="ACGI6B_002962"
     CDS             5664..6380
                     /locus_tag="ACGI6B_002962"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HFG9752379.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(6625..6903)
                     /locus_tag="ACGI6B_002963"
     CDS             complement(6625..6903)
                     /locus_tag="ACGI6B_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HFG9752380.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(7151..8485)
                     /locus_tag="ACGI6B_002964"
     CDS             complement(7151..8485)
                     /locus_tag="ACGI6B_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9752381.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(8498..9241)
                     /locus_tag="ACGI6B_002965"
     CDS             complement(8498..9241)
                     /locus_tag="ACGI6B_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752382.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(9262..9576)
                     /locus_tag="ACGI6B_002966"
     CDS             complement(9262..9576)
                     /locus_tag="ACGI6B_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752383.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(9580..9774)
                     /locus_tag="ACGI6B_002967"
     CDS             complement(9580..9774)
                     /locus_tag="ACGI6B_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752384.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            9914..10774
                     /locus_tag="ACGI6B_002968"
     CDS             9914..10774
                     /locus_tag="ACGI6B_002968"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752385.1"
                     /translation="MIDNTIGKKIKQYRKSINMSRDELANKIGCSVYAVAKYEQGQRK
                     PSLDMIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFDNYSAKLIGNN
                     ISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTKIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDKSEFDLVIHIL
                     KNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSN
                     GLIEDEDEEY"
     gene            10871..11623
                     /locus_tag="ACGI6B_002969"
     CDS             10871..11623
                     /locus_tag="ACGI6B_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9752386.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            11613..12779
                     /locus_tag="ACGI6B_002970"
     CDS             11613..12779
                     /locus_tag="ACGI6B_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9752387.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(12896..13126)
                     /locus_tag="ACGI6B_002971"
     CDS             complement(12896..13126)
                     /locus_tag="ACGI6B_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752388.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(13375..17946)
                     /locus_tag="ACGI6B_002972"
     CDS             complement(13375..17946)
                     /locus_tag="ACGI6B_002972"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFG9752389.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT         6165 a         2870 c         1839 g         7368 t
ORIGIN      
        1 atatagtaaa taaatttgtt attaaagcaa cagataagta tatatagttt attttattat
       61 ctttatctag tcccaatctt ttattaataa atttaaatat agcacaagct actatataac
      121 tattattata agataatagt atatatctac tttagcatta tcataactaa acactaatag
      181 agcttcctct gatgatatta cattacttgt aactacaaaa aataaattta tagatataac
      241 atcttttatt aaagggtgat atacgtcaaa taagaaaaat gaaactatat atttaacaat
      301 tcctatctta taaaaataat tatagtaagt tcccaagtaa taaatatcaa ataatagatt
      361 atatcatata gatttttctt catattataa tcatgggtct tatcaacatt aaataatata
      421 atgatcttag ttataagata agctaataat acagtaaaaa aagatatttt cttagtctta
      481 tctgtatctt tatcgtaagt taatatactt tttagtatca ttattggtgt aaacaatgat
      541 aatattaata atattttatt tatatggttt aacatattct atcaccaata aattttagaa
      601 acccctcttt tatatcttta tatctatatc tactaatagg tacttcttct tgactgtcta
      661 atattgcagt atttgacttt atattttcta catagtttaa gttagttata taacttttat
      721 gacatcttac aagttcctct atatttaaat ctttttctat tttttctagg atacatatag
      781 catcaagagt tttatttttt gtatgtatta gcatgttctt tttttgaact tctatatatt
      841 tttttgctag attagatttt gtatgtgtta gatttatttt tatttttttc aatttttttg
      901 gtacatgtta atatgtgttt ttctagttct tgaaaattta ttggctttaa taagtatcaa
      961 taagctctta cttcatatct ttcctgtaca taatttatta gagatgttat aaatattatt
     1021 atctactttt cttatgtttc ttgcgatatc tatactattt agttctgcca tttttatatc
     1081 taagagtagt gtatcctctc aattaacttt gtaatgattc ttcttttgag ctatattttt
     1141 ttaattcgat attctttgtt tttatttttt aatatattat caacttgatt ttttattacg
     1201 atttgtattt ctttttcatc atcacaagct gttattgtaa tgataacctc tcctaatatt
     1261 gtatttttat ttagattctt cactttaggt taaaactttt taaatgtatt tatcaataat
     1321 atttaaatta aaaatgtata aaaataccta tatccaattg gatataggta ttcgtggatt
     1381 tcaatggtgg agacgagggg agtctataag tggttatttg attgttttgt tgttttttgc
     1441 aagtgtaggt atatagctat ttagttatga tgttaaactg attttttcgg atatttttta
     1501 ttgtttattt ttgtagttgg ggtattattg gggggggggt ggatgatata atcttttaat
     1561 tttataaaag ttccattaac agcatattat acgtttttaa atatttacta gactttttca
     1621 aaataaatat ctaattattt aaatcaatac caatataatt tataaatgga tttataattg
     1681 tatcttctat catgtttagt gtaaacaact tattttaatt taaattcgta accaacaaag
     1741 taccaatctg acttataatt ctatattagt acaactatgg aaaaatttaa ttctatatct
     1801 cctcacttaa tcttatgact tctcttaaat ctttgtaata atcctctata ttcatttcca
     1861 tacattgctt tctattttcc tcagttctac tatgatccca ataaactcca aaattttgta
     1921 tagttgacac taagtttata catttatcaa accaatcttt aatttcaatt ggtggaaaat
     1981 ctaaatttat aactttattg taacactcat ccattttttt ctttctacta tctataattt
     2041 gaaaaatatt atcttcacat attttcttat tttcatattc attatagaag gatataattt
     2101 cttcagatat gtttatcata tttgttaaaa tttctttaac ttcgtatact gccttttctt
     2161 tagtgattgt attttgactt atatatgatt ttaagctttc gtagttatca tatgtttcag
     2221 tgtgaacacc gttgataaaa aatttatttt tcccatttac actataccat atatctttct
     2281 cttgatattt atcaatccaa gtagttttac acatatatat tccatttatc atatcaattt
     2341 catctttgcc aaaatatacc catattatat tagcatcttt tccttttaat ctatcttctc
     2401 ctatttcact attataaact tcaacagact taactatgtc aaaaacctcc ttagatattt
     2461 ctgatatatc acaataggag aatttatttt ttaatataac ttctactgaa tatcttttta
     2521 ttatacttat atgtaaagat ttgacattgt atattatgta atcgtcatgc tcttgaaaaa
     2581 ctttcttttt tatataagat ttataatctt ttacattaaa atatatatct cccagtatgt
     2641 tagacctaaa gataggattt tgtttacagt ttatataatt tttgttcaat atactcatac
     2701 gcaaattaat atacttgctt attgctctta aagataactc cccttttctt actatgtatt
     2761 catttcttaa cgcttctgat aataaacttg taaatctact aattgaacta tctggaagag
     2821 aaaatgattc ttgatattcg ctacttgata ctaacactgc acatcctttt ctatcaaaca
     2881 aggaaaatat gtctttaata tttatttttt gtttgtcact tacattaaat ttacctgcaa
     2941 agcaacaatc aataaataca attttatttt tacaatttaa tccttctata aaatcaatta
     3001 caaattgaat agaaactaat tcatcactta atactaagta atgcactccg ttattacata
     3061 tgcatccatg ccctgaaaag taaaatataa atgtatcatc atctcctata gaatcgctta
     3121 tttcttgaaa agttttaaaa aataaatctt ttctgacttc atactccata cctaaagttc
     3181 ttatattatt cgaagtaatc cccagtcttt tatgtaacac attattaatt tcaattatat
     3241 catttttaca aaatagtaaa tctttatttt taactatact atatttactt actcctacaa
     3301 ttaatgcttt taagcttttc atattccatt ccccctttat aatttttcaa tttaatatac
     3361 ttaaaatgat gttttaattg ttttattgat tttataaaag ctagtatcac actacttttt
     3421 tgactttttc attatttact tttgtagttg gattataatt tttcaaggta agtatatata
     3481 tatttattta catcgcaccc atataattta tatctctgat ttatctgacc tgaacttagt
     3541 gtaatttaaa ccaatgtaaa atcatctatc tcttcaccat atatttttta aaattcccca
     3601 aaatattgat gttaatagaa tttcatgtgt tttttgtatt ctactatcca taagaatttt
     3661 aaagtgagta aatatttcca atcatttata tcttatttaa tatcatttta ttccctcaag
     3721 tatttattta aattgttatg ctttatttat aaaattatac atacatttct aaagttagac
     3781 atacatcttt aaaaaccttt aatttgtgct actttttttc taaaaataat aaatatataa
     3841 aaattatata tacaaaaaat taagttatga ctagtttata aataagtaca taataaaatt
     3901 aaagagagca attttttttg ctctctttca aaatttatat taaattgtaa taataaaaat
     3961 aaaaacgcta gttatctaag gctaatattt tcaacgatta tataaatatc tacataacag
     4021 ttaaattaca tactcttctt cagttattgc aacttcctca gtgacatata ttttttctat
     4081 tttattaaga taatctacta tacttaatcc aatagccttt cctagcttaa caggaactgc
     4141 atttcctatc tgcttgtaca cctgactaac tgaccctgta aactcccact catcaggaaa
     4201 actttgaatt ctagcatact ctctagttgt gaatggtcta gtctcatctg gatgacacct
     4261 ttcagtttgc ttttgtgcag gactacatgt taaagttagg catggttcat cccaacttat
     4321 tcgccttgcc atacctcttt ttccgccacc gctataataa ctttttccca tataatctct
     4381 tgccacctca tctggcaaat ctatccaaca cccacctggt ggtaccatat ctaacacttt
     4441 tttcttcctc tcaggatatc tagctccttc cgattttgga acatttttaa gtgcatctct
     4501 taacgtagca acatattcaa acgacttagg gaacttaaaa tccacatgtg acaaatcttt
     4561 tcttgttcct attataataa ctctttccct cttttgtgca actccatagt tcacagcctt
     4621 tagtatctca tattttacat tgtatccaat ctcgctaaat acatctatca tagtctttaa
     4681 tgtttttcca ccatcatgag atacaagacc tctaacattc tctgctaaaa acaccttagg
     4741 ctttaactct tctagaattt tagcatatga ataaaacaat gtccctctta catcctctaa
     4801 ccctagcttc ttacccgcat agctaaatgc ttggcatgga taaccacctg atagcaaatc
     4861 tatctctata ttattatcaa tataattctt tattccattt tccgctattt taacaatgtc
     4921 accctgtata acattccagt ctggtctatt taattttaat gtatcacaag catatttatc
     4981 ccattctact aatccaacct ctttaaaacc agctttttca aggcctaatg ctaatcctcc
     5041 tgctcctgca aataattcaa cacatgtata tgctctttta gctagtacat tacttatatc
     5101 tatatgttta ttatttttat ttctctctct aggatttaat tcatattcac tgagttcttt
     5161 atatgtacag tctctatgct ctatataatc tttattactg ctacaaatat ttgtaaattt
     5221 cagttgcttt tcatttacat cttccaaaca cataatttca tcgaatccaa tatctaacac
     5281 ttcacataat ttttctacat tactttttat aggtgtaaat ttttctgata atattgcaga
     5341 taactgattc ttagttatgc ccatatttaa tgccagttga ttctgacttc ttatattttt
     5401 ctctttcata atttttctca ctctattttt atcaatagtt atttttttca tttagttcac
     5461 ctcttaataa ataatatacc cttaaatagt taataaaata cattaactat ttaaggatat
     5521 attatcataa ttctaatttt tatgtaagta gttataaaaa cttaaaataa aatatctgta
     5581 aacaacctaa tattacctca taattttaca cattatggaa tatattgtat aattacatta
     5641 atacttttta gggggattat tttatgactg aattagaaaa acaagaaata ctagataaag
     5701 ccaaagattt ttttacaaat attttggttg ctaatcataa aaagaatact attaagttaa
     5761 atttaaaggc atttaatata aatccttttt tagttaaata cttagctagt ttcttaacag
     5821 gatcaaataa acctgaagat attgctaaag cattgattta tccaagagtt ctaggcactt
     5881 cgattaatac gtccttcgga actaatttac aaaaattctg tattaccact ttagatggtt
     5941 ttgcctctac aacttctggc attgatatag agtttattga ttgtattgat ggtagaagga
     6001 aattctgtca aattaaagct ggaccaaaca ctattaataa agacgatgtt aaaactataa
     6061 ttgaccactt tagctcagtt aaaagacttg ctagaacaaa tggactttca ttatcttata
     6121 atgacttaat tgttggtgtt ttttatggaa cagaaaatga gcttaatgct cactataaaa
     6181 gaattaatga agagtatcca gtagttgttg gtcaagattt ttggtatagg cttactggtg
     6241 atgaaaattt ttattttgat ttaatagata caattgctca atctgcttta gaagaggact
     6301 gtactgtttt acttaaaaaa acaatatcca atctagctga tgaaatttct aaagataaat
     6361 ctttattcaa tcaaaagtaa tataaaaatg attaaatagc ataatttaca tttttagata
     6421 tttaaaaata atattcacca aacaaaatta ttaagtaaaa tgaaatgtat cttataaaag
     6481 ctgagctata agtaaatctt tgtaaaaaca aatttactta tagctttttt atgttcaaaa
     6541 aacacaaatt atatactatt taatatatta taaactttta tttgttaata ctctctacta
     6601 aatttctaac atctcttata taaattaact atttaataat tcaatcataa atctagctcc
     6661 tgccttaaaa ccaagcttat aaccaagctc tctttcaatc tctataactg aattaaataa
     6721 atcatttaac tctatcaata catccatttt cttttcttct gataaatttt tttctagtaa
     6781 tttattaact ttcttaattt ctttacatct atcattataa tactcatctt tttctagtac
     6841 tttcctatac atgtgttcat ttatccaata atcaaaatat atttctataa tttcatcata
     6901 catgttagta cctccttgac tttcatctgg tacttaatat atacttaact tgtgtagatt
     6961 agtgtacatt aagtacctaa tttataaagg tagttgttat atttatatgg caactacctt
     7021 tttctaataa atttttcaat ctaaatacta tatattttaa atcttcattg cttactattg
     7081 aggattatat acttttcttc taacactcct taaacaatag tttatatatc acttaataaa
     7141 ttatattttt tcatacctcc tttaccttct tacattcctt aaaagaaaac aaacttccta
     7201 tataccaata aggtctaaag cctgattccc catctctatt tttagcaact ataatttcac
     7261 ttaaattagc tccaaaacct ttttctctac gtcttaattc ttttatatcc tctattccca
     7321 ttgtcttaca ataatcaaga gcctctttct ctgtaggtct atgaatatat atgactgaat
     7381 tagaatccat atatatctgt tttgaacttc tcattactct atctcctttt ggcctttcat
     7441 ccttcaattc atcatttaat tgagccaact gtataattgg aatattaaag tctaaagtaa
     7501 tcttttttat ttgccttgat aaatcagcaa acttagcttc tccaacttct ttaccttcgt
     7561 aatccaaaat ctgaagataa tcaattataa caacatctgg tttaatactt tttatttctc
     7621 tcttaattcc tgatattgta ttgactgaat cattaatata gagattattt tcctgattta
     7681 aagtacttaa agcatttatg tatttaatta tttcttcttc atttagattt ctattcttca
     7741 tttttacaac tgatattcca gttaaagaag ctatgtttct aaacaaaatt tgtatatcac
     7801 tcatttcccc acttataaaa agtacttttt taccttgaaa taccacattt ctcattatct
     7861 gtaaggctag tgctgttttt cctcttccac ttttggctgc tattgtaatt aattcaccac
     7921 tatatagacc tccaatagtt tcatctaaaa attttattcc tatatcgata ccttcttcaa
     7981 tttcatcatt tatgtattca aaggctcttt ctgatatagc tgttatacta gaatccttct
     8041 taaccaattt ctgattaact tcattaacgt atttttctaa ttgatatata agtgtattat
     8101 catctatttc acctaatttg aatttattaa atttatcaat tatatttcta tttttatatt
     8161 tttcaataac tatctcaata taatgggata cattacctat agttggaact attgttgata
     8221 gttttgtcaa ataacttata cctccaacat aatctataag cccctttttc tttaaatctt
     8281 ctgtaagagt tattatatct ataggctcat cccttttatg tatatctttc actgaattga
     8341 atatatgttt gtgctcttga tgataaaaca tatcttcact tattaatatc tcaatctctt
     8401 ctattttcct agaatctagt aaaatactac ctaatataga ttgttctgct tctgtgttct
     8461 taactaaatt actcatatta tccataatat aaactcccta ccactttaat tgtgaatcat
     8521 ctggactata tgcttctgca tctactttga cattaattga tttatcttga taattgcctt
     8581 ctaatatctt aataaaatta tttgggctca ttatccaatc aaaatttgcc ttccaacctt
     8641 tttcattgct tccatttaaa aagctagttt tactaactat atgaataatt tctaatacag
     8701 tctctttatc atattctttt attctagcat taattacttt ttttctagct tctgtaagtt
     8761 tctttacttt tgatagttct gtacaagtaa tattgaattt ttctataatt tcactgtata
     8821 ttttactttt ttcattattt acattcttgt tagttgttag ttgcttgtta attgcatgtt
     8881 gattagtctg ttgattttct tcattagatt gatatagctc ccagttttca atagttataa
     8941 gcctattttt gtttgttgat ttatttgtta gaaatccata tctttcaaat ttctttaaag
     9001 cagttcttac attctgaata cttacatctt ttgaacacat atttgctata ctttccaaag
     9061 atgttattaa ctgtcctgat ttaacttcat attctttacc tccataaacc catttattag
     9121 gcttatgatt aactgacagt aataggttta tgagtatatc tctttgtttg gaattacaat
     9181 ctttatatag tgggctttct tttatctttc tatgaagctt tatccaacct agattactca
     9241 aatactatca cccacttaag tctataattt tataccattt gcattttcaa taaaccaatt
     9301 atctagttta ttttttatta tatacactct attatctatc catatacaag gaaatccttt
     9361 atttttagaa tgacataact gtctaaattt tgattcccct atgggataaa ttttagaagc
     9421 ttctttacaa gtgataagtt gagctttatc tcttctatat gtctgtatct ccttgtaccc
     9481 tttttccttt tcttgtgctt cttgttcttg cttgacaagt atattcaatg tagcttgtag
     9541 tgttttaaga acttctagat tatttgtatc tgccatacat cattcctcct tatcaaaaaa
     9601 taattcttct acacttgtat ctagttcttg agccaatttt aacatcaaca tattacttgg
     9661 attttttgat ttacctcttt ctaaagaaga taaataatat cttgagatgc caactttttc
     9721 agctaattca tattgcttta tacctttttc aattcttttt aatttgagtt tcattgattt
     9781 cctcctttct ttcataccca ttttgtgtat cctcatttct ataaaatatt ctaacaccca
     9841 tttggtgtat tgtcaatata tttaagtgta tcaatagacc catttagtgt tataatgtat
     9901 ttgaggtgaa attatgatag ataatacaat tggaaaaaag attaaacaat atagaaaatc
     9961 tataaatatg tcaagggatg agttagctaa taagattggt tgctcagttt atgctgtagc
    10021 taaatatgag caaggccaaa gaaagcctag tttagatatg ataaaaaaaa tatctactgc
    10081 attatcaatt cctataaata ggcttattat tgatgaaagt gatttagata cttcaacaaa
    10141 taattatact aatttaaaag aattggattt agttgtaaaa acatttgata attactcagc
    10201 aaaactcatc ggaaataata taagcaaatt tagaaatata aataatatat ctctcaaaga
    10261 actctcagaa aaaataaatt gttctgagaa tgaaattata aattatgaga atggaacaaa
    10321 aatgcctaga attgaaatat taaaaaaaat agcacttgct acttatacta agattgaaga
    10381 cttatttgga tttccattag aagagataaa aatagataac caaacaatcc tctatagaaa
    10441 tccccagact ggatatatta tagataactt tcttatcgat tttataaata aagatattaa
    10501 catagataat actccagttt cttattatat agataagtca gaatttgatt tagtaattca
    10561 tatattaaaa aattatggtt atgatgtaaa aacagattat aagacaaagt ttgaaattgc
    10621 agataatgca gatacaataa aaccaattag cttaagcttc tctgacttca aagacttctc
    10681 caaaaatcta aattgggtga taaatggtct tgttgaaaat tttatacaag aaaactctaa
    10741 tggtcttatt gaagatgagg atgaagaata ttagtattgc acattttcaa aatatgagtt
    10801 tacctattaa cttttataca ttcatagtta ataaataaaa attagatatg atatgaaagg
    10861 agctaaaatc atgaataaat catatatacc aaaatacaca cataaattcc ctactaatct
    10921 acctcatgga gataaaaaat atactttaac atacattaga agaatgataa gtgaatttgt
    10981 atatgatatg ggtaacctag aaaataaccc ctttacattc ccagaagtac aaacattact
    11041 tgatggtata accgtaggtg gacataaatt aagtgaccaa aatcaaatac taaatataaa
    11101 atcatcatgg gattatgtaa taaaattaag taacaaagaa aacttatctt taaataaaga
    11161 tacaatttgc tctatacata aaatagttgc aaaagatgaa gctctaatag ttggagattt
    11221 tagaaatggt aacataggta tagctggtac tactcaatat gaatgcatag aagccactct
    11281 attaaatgat ttatttgtaa gcgacataaa tatcataaat aaaataacta accccttaga
    11341 aaaggctata ataataaatt tatggttgtc ctattgccaa ttcttctatg atggcaacaa
    11401 aagaactgct agacttacct caaacctaat acttataagt aatgatatag gagttctatc
    11461 aataccagct aaacataaac aagaatacaa cacattaatg ctaaactttt atgaaacact
    11521 agaagctgat gaagttataa aattcttatt agagaaatgt attacattct ttgatgaata
    11581 taactataaa tcatataagg agctgtttaa aaatggcaac taaaagagct aatggagaag
    11641 gctctatcgg aaaatataaa gatggttggc gttcccgtat aatgattggc tataatgaag
    11701 atggtaaacc aataagaaaa gaattttatg gtaagactca aaaggaagtt aaagataaac
    11761 ttgatgctta taaaaaacaa tattatttaa gctctgatat ttcagatgat aaaataactt
    11821 tagagcattg gttttacaat tggttatttg aatatagaat aaaagattta aaacctaaat
    11881 catttgaaag atatgaaagc atctatcgta attatattaa agatactgat ttaggcaatg
    11941 ttaaattaaa ggacttaaga gtatctcaca tacaaaaata ttacaataga ttgcttgatt
    12001 ccaataaaag tatacctact ataaggcaaa taaacactaa actgaaaacc tgcttatcag
    12061 aagctgaaaa acaaggtctt atacaacgta attattgtac tatggtaaat ataccagttg
    12121 agaaaaagga gaataagctt gaaatattga ctcttgaaca acaaaagagt tttataaaag
    12181 ctatagatgg acataaatta gaggtattat ttttacttgc tcttggtact ggacttagat
    12241 taggagaatt attaggactt aaatggtttg atattgattt taaaaagtct aatttaacag
    12301 ttaaaagaac ccttcaaaga acatatttta tagataaaac cggaaataga gagcttaaag
    12361 tattagagca agagccaaag acatcaaatt cttacagaac tgtacctatt ccaaaagatg
    12421 ttttaaataa attgaaagag cataaagtaa atcaaaattc ggatatatta aaggctggag
    12481 aattatatag aaatgataat tatgtatttt gcaatgaatt aggtgcccct attgatgata
    12541 aaagaccttt aaggaatcta aaatctatat taaacagctt agatatagag cctataaaat
    12601 ttcatggtct taggaaaact tatgcgacta gactttttga gaatgatgtc cctcctaaaa
    12661 ctgtacaagt tttaatgggt cattatgata tatcaataac tttaaatata tatactcaag
    12721 ttatggaaga taagaaaatt gaagctgtag aaaagttgga taaaatattt tcattgtaaa
    12781 aatcaaatat ataaaaatac tctgatttca tatagttaat tacaaaacca aaataaggat
    12841 acaaattaat tttatattat cttgtatcct tattcttgtc tagtttttta aatatttatc
    12901 ttggatagcc tttatgatac attaatccat tttctaacat tagttcttgg gctaaatata
    12961 tatgaccctt acagtgcata tctgatatac cctcacttat aagtttatat gtttcagaag
    13021 tatataaata atccatactt tcttccaagc tcttacctgt ttgtttagaa aattctaata
    13081 tgattttttt atatagcgat tgtaacaatg tcttattagc ttccataaaa gctatacctc
    13141 ctctttcaaa tatttgttta tcctattttg actaactata tatttattag ttatttaatt
    13201 agccataata attcggctat aagatattta taatctaatc tcaattatat aaatcttttt
    13261 gccaatattt aataatcaaa atattgcact taattaacta tcttataagt attgataata
    13321 aaatctgtct tcttaaatat acttaaataa aacttctata attatatcaa agtattaata
    13381 ttcactctct attgtctctc ctacaagtct attttctcca tttagggtat atactcctga
    13441 tatagttggt tgtatgtatt ttctatccat atatggttct tctaaaactc ttttttctgc
    13501 tagcatcgtc caaaatacac tatatccttg ttcattaaga tattttaaga aaatttcctt
    13561 attaataaag aatcctatct cttcattaag taattcacta ctttcaaaac aaacaacttc
    13621 accataatca ttatatagaa tcgaatctcc ctgaccatat ctcaatttca acccataaaa
    13681 tagagtacta caaggtttat gccatgtgcc agaaaagtct tcttgaaata tgtcaccttc
    13741 tcgttcagta gtataactat aagttggcaa taatacttta taatcctttt ctaagatatt
    13801 cttatgttca tctatttgta cccactcttc tccacaataa tatgaatttt gaaaaaagta
    13861 atatccatct gcccaataat attccttatt ataaagagta taattactat taggttcagt
    13921 aagccatcta cccataaagt cttttccctt tagagcattc actattggtt ctagttggtc
    13981 atttttaatt atatagctat tgattaatac ccacatgtct ttttcaggat ttttatagtt
    14041 cttattttta aaatcatttt cctccttcca agtataccat ccattaagta ataaaaagtc
    14101 ttgatttttg taaacactat ttaccatatc atttatgctt ggtaaatcac taaaatctcc
    14161 taaccactca ttattaggta ttggagaaaa ttgaaataat tgattatgta ctcttttatt
    14221 gttattatta tttattactg tcatgacagt tggatcaata ttcctaatat ctggctcaaa
    14281 tgtaccctta caataaattt cttttttagt accataagca tctgtataca cttctatctt
    14341 ataattatca gcagcttgcg ctgcaagttc atataatgca atccattgat atttctttcc
    14401 tattctttca tatttactat ctcttaatct accattttgt actgttctat caaatttacc
    14461 atgtttattt acatcatatc ccatatcaaa aactttctta atagctatat tctttaaatc
    14521 gttatagtct agacctttcc atttatggaa atagtgttgg aacatatatc ttccaaaatc
    14581 tccataccat cctggttgtc catctctagt atattctact tccatagaat ttaagatagt
    14641 gttttgactc caataataat ctttaaaatc tgatgcctta tagtcatact tatatccctc
    14701 aatttcttca tcagttggta ctttaggaaa aattgattta tatggtggtt caacattcat
    14761 atcttttaat actgaactat caattttata ttttgcataa tctacaatat tttttgcata
    14821 atctcttaat aaaatattgg gataaacata atctacatca aatattttat tataaacata
    14881 aactgccaat gcctctatca tctcttctga ttgctctgat acaacacatc caaatgcaac
    14941 tgcatataat cgttcaataa tatatgggtc atctacatct tcaaatgttt taagtaaatc
    15001 tattacaata tccattttcc ctgttaatag atttacaaga gcttttgtac ttttatctct
    15061 taagtctcta tttgaagaaa ttaaaaacgt tgcaatcatc atagctgaca atctaattgt
    15121 ttcatttgaa gtattatcaa aaccttcaaa atcaaagcac catcctaata atcgcttaat
    15181 agaagactct tcttcttcat aaacttcatt aaatataggt ataaagatag catctctatc
    15241 tgccatagta ttttgtaaaa tatattttac tatataatct gcatttaatg tatgattaac
    15301 ctttgtagaa attaagataa gtacattata aaaatctatt ttataataag atttacctaa
    15361 aactatttta ttaatataat ctagtgtttt ttcagtaatt gtagatttct tacgccattt
    15421 tagtgcattg ataaatgcac taataacatt ataatttgtt tctttatttt taaaaacttc
    15481 aaaaatttca ttaggtatat ctgagcctgt ctcttcagat aaaacaattg ccaatacttc
    15541 taatatatcc tcatattcta ctaaatattt atacttatgt gaaaaatcta ctaccccaat
    15601 ttcttttaac tctttaacca acaaatttgc atataaataa tcttctaact tctcatatgt
    15661 cacatgcata tattcttcat catttaaatt gactgtttta ttaactattc catttgctat
    15721 gagttcatct attatcacat tgttaatctt atacttactt gcaatattta ttataatctc
    15781 tacactcttg tttgcgggta ttaaattatt cccaagacca tctccatatt tatatttaat
    15841 catttcatta agtacttctg taactacatt ggcatgtcta ctataattgc atgattcaga
    15901 tattctaaga ttaagtatat tcaaataatt tttataaata tctgtaaaac taatagtatc
    15961 taagttaatc tcttctcctt taaaagtttc acacataatt ctcaaaaaaa gagggtttct
    16021 aaattcttga tttggcattg gaatattaga ataaggtatt ccataataat caaaatactt
    16081 tttcatagct ttatgttcta tactagaaaa tccttgatgc tctataaaaa ccaaattctc
    16141 ttttatcttg ttattattat ctattaaaga tggtatatat tcacttctta ttgataatat
    16201 tagtccaatc catggatatt tctttatctg ttctacaata ccatttaaat aatatttcca
    16261 aagttctttt ccttgacctt cattcaatgc atctataaat aatatcacac gctgttgctc
    16321 tttttctcct atttcattaa gtatttctaa taattcttca aactcacact caatttctaa
    16381 tatttctaac atatgtttaa ttggtgtttt atccaactta aaatgttgcc ctaaaaataa
    16441 tatagactta tgcccatcca tatttctttt ctcaatagta tgtgcaatta tatgagattt
    16501 ccccatacca cctgcactat gtaataaaat ataagggcta ttaactactt ttatttcctt
    16561 agaatccaaa tattttatat atacatagat atctttttga gtctgcttca atttgtatat
    16621 ttcatcttct ctttttttac ttttttcaca gtcttcattt ataattatat ttttaaaatt
    16681 atttattcta tcatcaagtg ctgcacttat tttttcaaag tgctttttta acaacttgat
    16741 atctaatctt tcattagctt tgtcataaaa ttgttgtatt atttcttcta taactagtac
    16801 atctacttcc acttcattta ttttctgttt ctttatactt gatagaactt tatctgtttt
    16861 ttcatcaaaa cgttgtttaa attcttcatt ttttcctaat gcattaaacg tattttctaa
    16921 actcaattct acattcaatt ctggtaaata tctatctcca aggtttttta tctgtatatt
    16981 attctgattt tgaaaccatt tctctgttaa tctacaagat aaattagtta acctactttc
    17041 ctttaaagac ctatccttta atcttctata ttcatctggc aaaactattt ccaaaattcg
    17101 ttcaagtatt attcttacac actcatgctt accttcagga tacataatgg tttgtatata
    17161 atactcattt tctaactcta ttcgtctatg tttctttttc tcactactaa ctggaataat
    17221 agcataatga cccgttcctg cctctgatac ttgctgtaat aattccaacg gtctatcctt
    17281 ttccaaacta catccaagaa ataacaactc tttacttgta tatacttgtg taagcatctt
    17341 tctcaattca tcattactat atgctttttc atattgttct tttgttaaca caatagatgt
    17401 tgtaggctct gtaatatccc catgaatttt atacaatact aattcattat ttctaagtgc
    17461 attatttaat gcttcaaagt gacctggatg tgacactaaa actgttttat tgtgtaatcc
    17521 ataatttttt tctagtactt tatcataatt agtagttata attaatcctg agccaaaaat
    17581 tataggaatt aaatagacac atttatcaat aaaatcgtct ttatcttttg catccagata
    17641 acctgcccca aaaacttttt ttaaatgttt agaaaatcta actttcgcca attcttcata
    17701 taaaaattct gcttttttct cataatcctc tattttttca aataaaattt tatcctcatc
    17761 ttctaaatat tcttcacttg tgttatttaa aaatgtactc catgaaggat atatgtcttt
    17821 aaataacata gacatgcctg ctccaatata tgggactaca taaccatctt taattgcatt
    17881 taccagttca tcaaaatttt tttgattgta aggcttatta aattccataa tttcttcaaa
    17941 tgtcattatt ccctcctaaa ctatctctta ataatttcaa ccataattcc tattttataa
    18001 ttttattata tcattccatt ccattttttt actacaattc tatgttatag aatgaattca
    18061 cttacttaca tttgttaata atataaaact ttcttttaag ataattaaaa tttatacttt
    18121 taaattatgg ataaaaatgg aatgttataa cttttaatgt attactaata ttaacaattt
    18181 taaaaaataa tactattctt attttatgct ataattatat tttgcataaa tattattgat
    18241 at
//
LOCUS       DAYAOY010000088        18235 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYAOY010000088 DAYAOY010000000
VERSION     DAYAOY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.088"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..1491)
                     /locus_tag="ACGI6B_002973"
     CDS             complement(<1..1491)
                     /locus_tag="ACGI6B_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HFG9752390.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFN"
     gene            1627..1833
                     /locus_tag="ACGI6B_002974"
     CDS             1627..1833
                     /locus_tag="ACGI6B_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752391.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(1838..2761)
                     /locus_tag="ACGI6B_002975"
     CDS             complement(1838..2761)
                     /locus_tag="ACGI6B_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752392.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(3015..4715)
                     /gene="argS"
                     /locus_tag="ACGI6B_002976"
     CDS             complement(3015..4715)
                     /gene="argS"
                     /locus_tag="ACGI6B_002976"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFG9752393.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(5177..5590)
                     /locus_tag="ACGI6B_002977"
     CDS             complement(5177..5590)
                     /locus_tag="ACGI6B_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFG9752394.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(5658..8036)
                     /locus_tag="ACGI6B_002978"
     CDS             complement(5658..8036)
                     /locus_tag="ACGI6B_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFG9752395.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            8355..9806
                     /locus_tag="ACGI6B_002979"
     CDS             8355..9806
                     /locus_tag="ACGI6B_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HFG9752396.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(10010..12523)
                     /locus_tag="ACGI6B_002980"
     CDS             complement(10010..12523)
                     /locus_tag="ACGI6B_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9752397.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(12746..13567)
                     /locus_tag="ACGI6B_002981"
     CDS             complement(12746..13567)
                     /locus_tag="ACGI6B_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9752398.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(14085..14561)
                     /locus_tag="ACGI6B_002982"
     CDS             complement(14085..14561)
                     /locus_tag="ACGI6B_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFG9752399.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(14595..15173)
                     /locus_tag="ACGI6B_002983"
     CDS             complement(14595..15173)
                     /locus_tag="ACGI6B_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9752400.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(15277..17859)
                     /locus_tag="ACGI6B_002984"
     CDS             complement(15277..17859)
                     /locus_tag="ACGI6B_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFG9752401.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
BASE COUNT         5684 a         3252 c         1985 g         7314 t
ORIGIN      
        1 attaaaaaac tgtggtatat ttgatgtttt acccagtgat atatagtcat attttaatat
       61 atcgttgaat acatcattat ctatgttgat tttttcatta tagaaatcca tcaatatttt
      121 atataactga ttctttcctt gtgccaaatc aaagtagcca ttttcatcaa aatacttggc
      181 aaaaacctca aagaatttaa aagggctttt tttataaaat ctgcctataa tatatctcat
      241 agataacaca aagtttttag aattgtagta tctctccagt atatcttcta tatcctttag
      301 cttcaaagtt tcttcatagc ttatataatt attgtataaa aactcatatg gagcataatc
      361 cttatattta aaaccatgct catctgcaac ttttctcata cctgtccctt taatcatctt
      421 taaaaagcct aattgcaaat gctctatacc tagattaaat acatcattaa aagaattttc
      481 aaagctctta taatcttcat aaggcagtcc tgcaattaag tctaagtgct gatgtatatt
      541 tctataagat gctacagttt gtacaacatg agacaacttt ttaaaatcat cccttctacc
      601 tactgcatca agtgtttttt cattcgttga ctgaacacct atttcaaatt gaaaaagacc
      661 ttctttacaa tcagccaaaa actcaagcat cttatcatct atcaaatacg ctgtaacttc
      721 aaaatggtaa gttgtatatc cattatcatt tttcataagg aaatccatga tttccatagc
      781 cacttttcta tttgcattga atgtcctatc tataaacttt atctgtgata ctcttgcatc
      841 tattaaagct tttaaatcac tctttatcct gtctaaacta aaatacctta gacctggaat
      901 agtagatgat aaacagtatt ggcagttaaa agggcaacct cttgatgatt cataatacac
      961 tattctattt tcatattctt ttggattcaa gttttcataa ggactaggaa tctcatccaa
     1021 atcttgaagt aattccatag gcttattcac tattatctca ttgccttgtc tatatacaag
     1081 tccatttaca tcatttattt ccatctttcc ttgcaagtga agtactaagt ctctaaaaat
     1141 taattctccc tctccataaa gtatataatc cacaaaatca tactttttca ttgcctcata
     1201 actatcataa ctcacttccg gtccgcccaa agctatttta gtattaggtc taattttttt
     1261 tagattatca caaagtttaa ctatatctcc cacattccag atatatgttg agaataatat
     1321 tatatcatat tcatttttat atatatcctt taaaatatag tccaaatcgt tatttatcgt
     1381 gtattctttc atattaactt ctatcaaatc tcttacaaat tcttttagat atcttatagc
     1441 caaatttgta tgaataaact tggaatttaa tgttgttaat aaaattttca aatatttctc
     1501 acctcttttt aagattatac tgtctttttg taccccttat aggttaaaat agtatatgta
     1561 aagtctaaat aatatctata taattaattt attaatttca agtaatgtga ggaaaggaag
     1621 ttttttatgg aattaattga aaaagtatct atcaactctt tatcaaaaag agatttacta
     1681 cttattataa agtctcttga atacactaat gaaaatactc aaattagtga tttcattgac
     1741 cttaggaata atatagtaaa ggaattatgt tttttaactg acactaagga aaaagatttt
     1801 ttagattatc tagaaaaaaa tagcacactc taataaccta aaccttttta aactttaata
     1861 tataaatacc tgatttagaa tttattttta tgcttaatat attatcatta aaggttttcg
     1921 acataactgt tatttgatta aatatagggt ctattatagt attaccttct tcaagtctta
     1981 taagacttct tgggtctatg cttttattat atgccttaac tccaatttta tattttctca
     2041 atttaacaac atattcggac atagaaaaaa taaaatctat attatcttca tttaatttac
     2101 tatcttcacc caaaatggat aatatagatt tttttatgct attaattcct tcattttcta
     2161 cagttctatc ttccatatat acatcatata gaacatttaa aacatttaat aaaatacttt
     2221 cttcatcata atcttcagat ttcaaatcaa tgtacttatc aattaattgt attgctttaa
     2281 tccttgccat ctgaaacttt acaacatctg atttgtctaa ttctatagaa aatcctatta
     2341 tcttgtcttt aatactctgt agtaattcga catactctat tttactatta tttataagat
     2401 tgtgcataat ggaattatac accacagaac ctaataaata atcaaataaa agttcttctt
     2461 ttttaaaata aataacatac ttttttatta gtttaataac ctcatcttca cttttttgaa
     2521 tattagtatt tgataaaatt agtggtaata aaatatttaa aaaattgata tttcctattt
     2581 tttctccata aaatctatag taaataagtt catctctcag tctattttcg tctatataca
     2641 cacatctttg cgttttatca tctattaagg attttgtatt tattatagtt aaatctttaa
     2701 gagccctact tgcactacta aaagaagatg tgtagacttt ttttagtaat tcgtcattca
     2761 taaatatatc ctttctgaaa aattgaatgt aaataaattg ttattaaaaa aaaagagaca
     2821 gcccgtttaa gaagctgtca tatatagggg gatagtagtg tacaaaaatc attgtaatct
     2881 actatctata tattattata cgatactact tttttatatg taaagcatca gttttcgaca
     2941 tccctatatt ttacagttat acaaattgcg tactaagttc aattttattc tttttatttc
     3001 aacattttat catgctacat tctttcagga gcgtgcattc ccaacaattt cagagaattt
     3061 tctaaagttt gttttgttgc taaaactaat gcaagtcttg cttttcttat ttccaaattt
     3121 tctactaaaa ttggattgtc atgataaaat ttattaaacg cttgagctaa atctagcata
     3181 aatctagtca ctatatgtgg ctcatttttc tttagagcaa gtaaaatatt cttattgaat
     3241 gattctataa ctctaagaac ttctgcacta tcttcatcag ctaatagact ataatctata
     3301 tcagaagtca cttctacttc tgcttttctt aaaactgcac agcatctagc atgtgtatac
     3361 tgaacatatg gtccagtctc accatcaaaa ctaagcgttc tttcccaaga gaaggtatag
     3421 tctttaattc tactattaga taactcttgg aatactactg ccccaacacc tacttgctta
     3481 gatatttcat ccacattttt agcatttggg tttttagcta gtataatttc cttagtttta
     3541 tctatagctt gatttaaagc atcttctaaa aatactactc ttccttttct agtcgacata
     3601 gtaccttctt caagtgcaac cataccaaat ccaacatgaa ttaaatcttt tgcccaatca
     3661 tatcccataa gctcaattac cttaaaccat tgttggaaat gtaagttttg ttgagaacca
     3721 actacatata tacacttgtc aaaatcataa gtttcttttc tatatattgc agcagctaaa
     3781 tctcttgtca tatataacgt tgagccatca ttcttagtaa taagtgctgg tggcatttta
     3841 tactcttcta aatcaacaat tcttgctcct ttagactctt ttaaaagatt tttctcttct
     3901 aatagttcga taactctgtt cattttatca gaataaaaac tctctcctgc taaagaatca
     3961 aattctatat ctaaaagttt atacactctg ttaaactctt ttaaactctc atttctaaac
     4021 cattgccata attcttgtgc ttcttcgtca ccattttcta atttattaaa ccatgctctt
     4081 gcttcatctt ccatttctgg gtgttgttca gcttcatcat ggaatcttac gtataatttt
     4141 agtaattctg gaattggatt agactctact acttcttttt ctccccactt tttaaaggcc
     4201 actattaact taccaaactg agttccatag tcacctaaat gatttattct tatagtcttg
     4261 tatccttgcg aatcatatat cttatataaa gcattaccta taactgtagt tcttatgtgc
     4321 cctatatgga aaggttttgc tatatttgga gaagaatatt caactattac agttttatct
     4381 tttccaaaat cactatgtcc gtaattttct ttttcatcta aaactttttt aattacagtt
     4441 tctgctaatt gagatttatt tacaaagaaa tttacatatc cgcctaattg tattactttt
     4501 gttatctctc cacttggttc tatactttcc gccaattcac ttgctatcat gtttggagct
     4561 tttctaaaca ctttggctaa tttaaagcat ggaaaagcat agtcccccat atccttattt
     4621 ggtggaactt ctataagtgc ctctatctct tctttcgata aatcttctat tttttcttta
     4681 aggcaatttg atattgccac cttaaaatct tgcattctgt tgctcctctc tacattgttc
     4741 ctggtttata ttgtgcttat ctctttatgt taaatcaaat ttcgtgtttt ttcaagtatt
     4801 tcattacagt gtaaaacact ttagtaatta tgttttctgt atatattaaa aatactataa
     4861 ttgcagtttt taatcacact attattgatt ttttataaga cttttttcta ttttattgtt
     4921 tcttgtacta tccagttgac tttatatact ttttttaatc ttttatctat tttcatacgt
     4981 caatgctaat tttatatact tttcttttaa tcttttatta atttttatac atcaatgtta
     5041 actttatata agttttttta atcttttatc tgttttcata catcaatgtc agctttatat
     5101 acttttttta cttttttatc tatttttata catcaatatt agctttatat acttttttta
     5161 atcttttatc tattttctat aaatcttttt cactcttaac ttttattcct tcttcttcta
     5221 atagccttgc tgttatacct ttccctggta tctttttacc tgaaaaacta ccatcatata
     5281 ttagattaca tccacaagag gggctaccct cttttagtat tgcttcgcta catacatata
     5341 aatttgctat ttttagagtt tcattagccc ctcttacaaa gttttctgtt acatccttat
     5401 tatctttagt aattactttt ccatcaacta tctcagcagg tggtcttggt gtagatagac
     5461 cacctaactg ctctgggcat acaagtgtaa attctttgcc ttttaaatat tccttgactt
     5521 tttcattatc attattatcg ccactatatt tacaatttat tcctattaaa catgcagata
     5581 ccaatatcat aaaaatgtgc tcctatacaa attcttaata tattctcata caaattacta
     5641 atgtattctt atacaaatta ttttaattta acaattgtta ctcctgctcc accttctcca
     5701 tattgaccac ctctttgtga ttttacatgt ctatttcttc ttaaaatgtc ttgtagccct
     5761 gcttttaata ctcctgtacc tattccatgt attactgtta cagactctaa acctgccaca
     5821 tatgcatcat caagatactt ttctacttcc attatagctt cttctaggtt taaccctcta
     5881 aggtcaactt cattttttac actaccagat tttgacctta taatgtttct cgttgattta
     5941 gttacatttg ttgaaacatc tttgcgtgtt ttttgtaaag acttaaaagg tagagtcatc
     6001 ttcattatac caatttgcac tactgcttct tttctctttt tatctactga aactacacta
     6061 ccattttgat ttaaagttat taccttaact tcttcacctg gctttaagtc ttttatctcc
     6121 ttatttgata cctttggaac tatcatactt tttactgttg gctgaagact tcccatagag
     6181 ctggtcaatt cttttctaag ttcttctatc ttacgattct tttcttttga agctctttct
     6241 tgttcaagac ttctaagttc ttttattata atatctactt cttctttagc ttgtcttata
     6301 attgaaaatg cttctgattt agctttttca atcatcttat ctctttgact gaccaatttt
     6361 tcaagctttt cgtcatactc tactttcagt ttttcaatct cttcttttaa tctttcagct
     6421 tcttctctat cttctactgc tttgattcta ttcttttcaa cattctgtaa cacatcttct
     6481 aaagctatgt tttctgtatt tatatattct ttagcacgtg aaatcacata atcacttagc
     6541 ccaagttttc tagaaatctc aaacgcattt gattttccgg gaacacctat aagtaactta
     6601 tatgttgggc ttaaagtttc tatatcaaat tcaactgctg cattttctac acctggttta
     6661 gtaagagcat aatttttaag ctcactatag tgagtggtag ctatacattt agctcctaca
     6721 gaatttatat cttctaatac tgctattgca agggcagctc cttcaacagg gtctgttccc
     6781 gctcctaact cgtcaaatat aactaaagag tctgctgtta cgttttgtaa tatagatact
     6841 atattcgtca tatgagatga aaatgttgat agactctgtt caatactctg ctcatctcct
     6901 atatctgcaa atacattatc atatacacac atactacttc cataatcagc aggtatatga
     6961 agtccacttt gtgtcataag tgcaaataga cctacagttt ttattgtaac agtcttacca
     7021 cctgtgtttg gacctgttat caccaatgta tggaagtctc ttcctaaata aattgtattt
     7081 gctactacct tctttttatc cagcagtgga tgtcttccat tttttatgtt taaatattta
     7141 tcctcattta aagttggttc tattcctctc atctgtatag aaagttttcc ctttgaaaat
     7201 gcaaagtcca atcttccaag tatctcttga ttagaaatta agtcttcact aacttctcca
     7261 accatagcag aaagctctga taaaattctt tctatttctt cttgctcacc tagttttaac
     7321 tgtctcaatt cattgttcat ctctactata gtcattggct ctataaaaag tgtcgcacca
     7381 gaagatgact ggtcatgcac tataccagct acttgcgacc tatattcaga ttttacaggt
     7441 actacaaatc tatcacctct tagagaaatt attgcatctt gtaagtactt ttgataagta
     7501 gttgaactta ttattgaatt taattttgac ctaatggatt gatttttttg ggctattctt
     7561 cttctgatat ctctaagtgt acttgaagca ttgtcagata tttctacttc actaattata
     7621 gcattgtata tctggtcttc tatgtccttg tatacatata gagaatttga tagagattgt
     7681 attattggat aattaaaatc ttcttcatca ctactagata agctatttct taatcttcta
     7741 gccactctta aggtatctgc tatcataatc agacttcctg ggtcaagaga agcacctata
     7801 taagctctct ttaccttatc ttctatatca tgaatacctt ccaaccctac tgaacctctc
     7861 tttataataa ttgattgagc ttcactagtt tcttctagca tagatttcac ttctacaaaa
     7921 tcagtatttg gtactaaatt ttctatatat tttagaccta aagaagatga ggctttcttc
     7981 tttaacaaat ctattatttt attgtattct agaactctta acgatttctc attcattttc
     8041 tatattcacc atcctcgata ttttaacttt tttatttaat tttaaaaatg tatgttttca
     8101 agcatttcca ataaaaactg caagttttat ttctttttca tttctaaaat tattatattt
     8161 tttctctttt agatatattt tacacatatt ttgtattata ctatgtatgt agctatctat
     8221 atattataca taaacatgct ttatttgtaa tataatctgt taaaaaatct tctttaatat
     8281 ttttaaagga gtttatttta gcttataaaa aaaaatagct acttatatag ataaattaag
     8341 gaggaacatg agtcatggga aacgtattag aaggattaaa accagaaagt gtttttaaaa
     8401 actttgagaa aatatctcaa attcctagag gttctggaaa tgaaaaaggc ataagtgatt
     8461 ttttacttag ctttggtaaa aatttaggtc ttgaaactat tcaggatgaa agcttaaaca
     8521 taataataag aaagcctgct acaaaaggat atgaaaattg tccaggtgta gttcttcaag
     8581 gacatatgga tatggtttgc gaaaaagaaa agaatgttga acatgatttt cttaaagacc
     8641 ctataaaatt aagaattgat ggtgacatga tatatgctac tggaactact ctaggagctg
     8701 acaatggtat agctgttgct atgggtatgg ctatattaga agataatact ttagagcatc
     8761 ctgcactaga agttttagtt actgttaatg aagaagatgg tatgaatggt gcagatgcac
     8821 tagaccctag cttaattaaa ggtcaatata tcctaaatat ggactccgag gaggaaggat
     8881 acttattagt tagctgtgct ggtggtaaaa cttgtgttgt tagtttacct gttgaataca
     8941 aggaagttaa aggtgacaaa caaggattat tagttgaggt tactggacta ttaggtggac
     9001 actctggtat ggaaattgtt ttacaaagag caaatgctaa caaagcaata gctagagtat
     9061 taagtgtatt gaatgttgat tatgaattag cttctgttga tggtggtact aagcacaatg
     9121 ctatacctcg tgaagctaaa tgtgtaatag ctgttaataa agctgatgtt gaatctgcta
     9181 aaaaacaaat aaatgacatt ttaactgcat ttaaacatga gtttactact tctgaccctg
     9241 gcatgactta ctcagtagct gaaacttctg tagacaaagt tcttactaaa gattgtaaag
     9301 aaaaagttgt tcaaatgtct tgcttaactc ctcatggagt tcaatcagta agtcttgata
     9361 tagaaggatt agttgaaagt tctactaact tcgctataat cgaaactaaa gaatctacta
     9421 ttgaattttt aacttctgtt agaagttctg ttatgtctat tagagatgaa atcgctgata
     9481 gaataagact tttggctcaa gctttaggtg ctaattacga ccttatagct caatacccag
     9541 cttgggaatt taaaaaaggc tcaaaattag aaaaaatatg cagtgaaact tatgagaaat
     9601 taactggtaa agttcctact gttatggctc tacatgctgg tcttgagtgt ggattattac
     9661 ttgataaact tcctcatgct gaagctatct ctataggacc agatatgttt gatgttcata
     9721 ctccaaatga acatgttagt atcccttctg tagctaatgt ttgggattat gttattgaaa
     9781 tattaaaatc tatgaaccaa tattaaattt aagttttgat tctaagtaga ttttaatttt
     9841 aagtaatcta tataaaatta actctgtaaa ttaatactac tgttagtata atatttactt
     9901 tcaatattaa cttatagaaa taaagcctaa tgatagataa taacatgtat cattaggctt
     9961 tattgtaatt taaatattat taagctttat tctgttttaa aattaattct tatactattt
    10021 taaatctatt tcttccttgt ctcttagaat cataaagagc attatctgct atttgataca
    10081 gttcatcata ctcttgacca ggctttgcta taactacccc catactaata gataaacttt
    10141 ctccatttgg caacttaatg agatgaatat gattgtatac atcattacat ttattaataa
    10201 gttcttcctt atttattata tcattgatat aaacactaaa ttcatctcca cccaaacgac
    10261 ctactatatc attttttgca aaactttcat caagtacctt tgcaacatca ataagtactt
    10321 tatctcctaa caagtgacca taagtatcat taatcgactt aaaataatca atatctataa
    10381 tcaataaagc accatttttt tcagttgata aattcaacaa attttgtgca gttaattttc
    10441 taatatatgc tgcattataa atattggtta aactatccct ttgagcctta tctaacaatc
    10501 tattcttttc ctctttttca gaatcaatat tacatatttt cccgattaca tacattggta
    10561 tttttctatc attataaatt atttttgaag taactctaat ccaatgaaaa gtatcatcaa
    10621 gcataataga atacatttca gttgttccat tttcttgtga ttttattaca tcaaaaaact
    10681 ttttattata ttctttcctt attttatctc tttcattttc tatattgcca ttttcacttt
    10741 ccttatcatt tttatccaaa ccataataag tctcaatacc cttttctggc atagaaatta
    10801 tcaatttatc ttgcttataa tcatactcaa aaaagtattc acttgtcatt tcatacaact
    10861 gagtatgttt tttgagttct aaagatatct tctcgttcat acttgtacga gtatgcaaac
    10921 caaatgccat aaatccaata atacaaatta ataaactggc tacaataaaa atagtctcaa
    10981 ttggattctg ttgcataata taaagcaacg agaagtcttg tctatatgta gtattttggt
    11041 agacaattga ttgcattttt tctgatgaaa tacttataat agctttatta aatatattca
    11101 ataattcttg tttatctttt ttaattatac caaaacaaat actctttgct ttataagttt
    11161 gaggaactag acgcacattt ttatacttag gttgattcat atagtactgc atagaataac
    11221 cttcgccata agtgtaatct gcatagcctt tattaacagc ttctatacac tcttctaaat
    11281 tatcatacca aactatattc tcttcaaact gaccataata attaatacct ttggctagag
    11341 ctacacgttt tccatctaaa ctattctcat ttaatttatt attaatcatc atagtatatt
    11401 gagtagaaag aaaaggccga gacataacta tattatgttc acgagagtat tcataatcat
    11461 aaatcattcc tgctacaata tcaatttcac gctttttagt catgttttct aaatcttctt
    11521 gagattttac aggcacaagt tcaaattcta agcctgtttt atcacatata tgatttaaca
    11581 aatcaattga tatacctctt aattcacctg tatctttatc tatattttga aatggtggtt
    11641 tgtttaaaag tactcctact tttaattttt ctgtctcttt aatgtaatca gtttcttcat
    11701 cagataaaaa catttgattg ttctctggtg taaagtattt ttcatataaa gtagttgaaa
    11761 aatatgggtc tttctgttca atattattaa ttgctgagtt aagcttttga gttaaatttg
    11821 tactaccttt aggagtaatg aaataaaatg gttttgatga aaattttgct acagtacgta
    11881 caccttctag tgggttcata ctaacattaa gaagtacatc tgctttacca ctctttagtg
    11941 cttctagttg ctcatcattt gttttacaat ataccaattc aggtgacata agattcattt
    12001 tacaatactc atctaactct ttaagtctag tatcagaatt ttctattaca gcaatacgga
    12061 tttttttcac atcttgtgaa ctcattatgt tatcaatatc tgtattttca tataatgctt
    12121 gtagtactgt atttgctact ccataactat ttcctgcata attatatatt ttttgcattg
    12181 gctcattgta taacatacct cccattatat caatttcacc attttctaac atcttcatta
    12241 attttgtaat agattcattt atctctccag gaacttgaac aaattcataa ttccatccag
    12301 tatactgtgc aatttctagt aaatattcat atgtataccc aaaatagtta ccttgctcat
    12361 ctatttcagt taaacctttt tgaataggat aagcaacacg aatggtacga ttatcattac
    12421 ctatagcgtt gataccaaaa gaaaatatgc aagaagtgaa tatagatgta attacaataa
    12481 ttaataaaag taattttttt aaattcacta cacaatcctc catatagtta agatttacag
    12541 tataaattac tgcataaact tgtagacatg taaaaataga ttagtatata aaaggatata
    12601 taaatctact tagaaattta aatatcctta atatatacaa tatatttaaa ttataactca
    12661 tttatatgtc tattacaaca aatttaccct gctaaatacc tactctatat cccataattt
    12721 tacaagttgt agtgaagttt aagttctatc tcacctcaaa tttagcagtg tttctattta
    12781 cagttctttt atatttcaca tctggataac ccaatataaa tgaagtgata acagcttcgt
    12841 tctcgctcat tcctaacagt tcttttatct ttggctctac atttgatgcc ataacaaaaa
    12901 atccattata acaaattcct aatccttgag aatatgccat aagttccata ttagatgcag
    12961 ccagtcctcc atctacataa gcacttccac ctagagacat atctcctaca actaccataa
    13021 gtagtggggc atcataaaat aaagaatcat ttccgttctc tttatatctt ttgtacattt
    13081 tcttaaatgt atttttatag atagaacgta cagggtcatt gtcatcagta tctaatgcca
    13141 aattataaag accttgtatt gccagttctt tcacttcttt tagcttttct tttactaata
    13201 tgtatcttat aggctgtcta ttaccacctg ttggtgtgta tctaccagct tccactatgt
    13261 tttttatttt ttcttcttct acctctatat ctttatattg tcttatactt cttctaaatt
    13321 taataaaatt caataacttt tcgggttcta attcaaaact ttcacgattg tattctatta
    13381 cttcattttt atcaaatcct tgcatttcta ttgcatctgt tgggcaaatt gccatacagt
    13441 gaccacagtt aatacagaga atatttttaa cctttgcttt atcatcctta acttttattg
    13501 catttacaag acagtctgat tcacacatcc cacagcctac acatttttct aaatttatgt
    13561 ctatcattcc aaattattcc tccttttaaa aaatttactt tagtataaaa caaatagttg
    13621 tctaagtcaa tttttatttt tttaacaata tactagcctt aactacactt taatggagaa
    13681 aaatatacat aaattggaac ttaaattaga acttattcaa agtaaaagat aaaagaagta
    13741 gatgtctaaa agtgaacttt ttagttaatt agatattctt tattgtatga aatcaagtgg
    13801 atatttatca tttcaacaat aaaaaaggct tacctctatt ttggatatgt ccctgtcaag
    13861 tagacagact aaaaaaaggc tatctttatg cactgagata gccccttttc ccaactacta
    13921 ttgtttatct taatattagt caataaattt aatttgctat aacctgtaag ttacatttta
    13981 atttgatgta actttaaatt acatttttaa gtaatatctt taaattatat tcttaaaata
    14041 tatcattaaa ctacatttta attaatctaa aaactatatc ttaattaatc taagaaactt
    14101 acattataat cttttagttt cccattatca tatacaatct ttgcactaaa gatatttcca
    14161 gtcttagccc aatttaagaa cagcctgtca ccttcccaca tatttaaatc gaatatttta
    14221 tcattatcaa cccaaattaa atttccttct ttacaatcat ttaaatcttt ttcagataat
    14281 tcaccttcaa actcatctga gaaatacaaa aacatatacc aatcttcttc atctttaaat
    14341 tttggaaaag ttatcaaccc tttatatgac aatgatttaa gagtaagacc agtctcttct
    14401 tttacttctc taattacaca ttcttctgga gtctctcctt gctcaaactt tcctccaact
    14461 cctacatact taccttcatg tatatcatct ttctttttat ttctgtacaa catcaaagtc
    14521 ttgtcatctt tttctatata acaaatagtt gtaagtttca tgtatctcgc cttgctttct
    14581 ctttatattc tcaactaatt attttcagca ctttcaagca catcacttat attcttcatt
    14641 gcattaagag aaagctctgc aaattcatca aatggaatcc ctatatcttc tatacttctc
    14701 attgcatctc tatttacatt tgaagcaaat ctcgtttctt tcattctttt aataacagac
    14761 ttagtcttta cactggtaag ttttttatct ggataaacta aagctatagc agttataaac
    14821 ccagttatag ggtctgctgc atatattgct ttttccatta ggctagttct tgtaactccc
    14881 attccatcat gatgaccaag tattgctcta tacatttctt cgtcaccgaa gttttctttt
    14941 ttaagcagtt ctactgttgt tgtagcatgt ccctcacata tttctcctgg ctttcttcca
    15001 actatatctg aatccaaatc atgtaaaaga cctgccactg cccactcttc ttctttttct
    15061 ggctctagtc ttttggctaa ttctctcata acagcttcaa cagcataaca atgttttctc
    15121 attctatcag tttctaaata cttaaataaa atttctaaag cttcttctct cattttcacc
    15181 aatccccctg ttttttcaca tatatgatat ttttatatat taatacacat aaattcaagt
    15241 ttttatatat attcatgtat atttttcatg ttatatctat tctacacctt tatagtagtg
    15301 tcctgcaact gttccaattt catctaattt cttatacaag tctgtagccc tttttccaag
    15361 tttataatta ttactcaata cctcaatgtg agcttcaata acctttttaa ccatctcact
    15421 atcttcaact gtaaaatcta tcctaaatac atctattcca atttcctcta actcatatag
    15481 attgtccaac atacaagtaa ccttcgaatt gtaaattgta cttctacaga atatatcttg
    15541 tgttatccta taacttgcat ccgtaaagtc tcttaaagca taactacttt ccttacattt
    15601 agcaactctc ttgtcctttt tacaatccct aacaactact cccataggac agtattctgt
    15661 aaccatcata ggagcatatc catatattac tgcttcaatt tctacatctg tatattttaa
    15721 agtttgttta atttcattta agtttagttc ttgtgacaaa caaactgtac ttgctccctc
    15781 ttctttgaag tggtttatac tttcactatt gaatgcattt aagtagcaat ctatatttaa
    15841 gtcttttttc ttaaagtatt ccatagagcc ccagttacca atttgaacct tattaattcc
    15901 ttttatatta tccaatatct caaattgctt gtactcttta tttctaacaa tccttggtgc
    15961 tgaatagatt aattttttgt cagtgactgc tgtattagag tggttcttat tcaaagtatc
    16021 tattttactt gtatttgaca aagtatatat gttatttgta tttgaaccca ttagttcttt
    16081 aggaatacct aaagatgaag aagtatataa atccatagaa atacttgaat ctatagaaat
    16141 acttgagacc atttgtaaag cttttgataa tgtatttata tcttcataat agattaaatc
    16201 tacattataa tttaatactg cttctaattg ctccaagttc ttaactttaa ctctaatctt
    16261 aggaactgta tcaagttttt tctgtttatt tactctattt ggctggtatt ttaatctatt
    16321 cttcttaaat tttctatctt ttatctttat tctttcttca tctaaaagct caatacaatc
    16381 ccttctcatc tgatttaaca cacttatagg catacttaca ccttcatcca ggtcaatttc
    16441 tatctctttt aacttatatg gtgtatttcc aagcttttga atttgtgttt ctattttttc
    16501 tttacttaaa gccaccttta tagctttttc aactattttg tctccttcta ccaccgattt
    16561 atttccacaa aaatcactta taaaaagctc tggcttttta cctagtttaa tactcacttt
    16621 tgcacgtata ttagttttta tgaactcttt atcttgctga aatgtagctt gtactctatc
    16681 catcaattcg ctgtcagaag tcttatatat aagctgattt ctctttgctt ctccaacaaa
    16741 atctaattct atcaaatccc ctttatgtgc tattgtagtt atctctccat cctttattat
    16801 tcttccaata gtacctccac ctaagttaat tccatcacct ttcttaagcg tattttctag
    16861 cattactcta agtcttttgt tctttctatt ataatctaaa acttttccaa tatatagccc
    16921 ttgattatta ggtctattag agttcattat atccttacca acttcaccta atatatatcc
    16981 ttttgtaaac tttctattaa atatggtata caaattattt attatttcct cagaaatact
    17041 tatttttttc ttatctacaa attcattaat cgcttctcta tatcctgaaa ccacagtagc
    17101 tacatactct ggctttttca ttctaccttc aatttttaaa gataatacac ctgcttcaat
    17161 tattttatcc aaatcttcta tagtacttaa atctctagga ctcaacaaat aatctccatt
    17221 actttttact acttctcctg tatttatatc tatcaactca tacttttgtc tacatggctg
    17281 tgcacaccta cctctatttc ccgacctatt gcctatcata ctactcataa ggcattgacc
    17341 cgagtaacat acacataaag ctccatgtac aaaaacttct atatctacag ttgtattttc
    17401 acaaatccat tttatctcat caacatttaa ttctcttgct aaaacaactc tatcaaaccc
    17461 tacactctga aggtatttta catcttctaa ggaatgtgct accatttgag tgcttgcatg
    17521 tagttcaaag tcaggtaata gtttttttat aagtcttgcc attcctatat cctgtactat
    17581 taaagcatca atatctatat cgtataaaaa ttttacatac tctacaaaat cttctatttc
    17641 atactgtttt atcaatgtat ttacagtaac aaagacttgc actcccctta tatgagagta
    17701 tttaacagcc tcaatcagct catttctatc aaagttattt gcagatgctc tagcactaaa
    17761 gtctttacca cctaaataca cagcatttgc tccgttttga actgctgctt ttaatgaatc
    17821 aaaagagcct actggtgcaa gtaattctat ttttttcata tttttctcct ctatttatac
    17881 ttgttaggtt aatataatta tatctataac ttaaatattt gtattaaatt tatatatttc
    17941 ttactatatt ttaaattctt caatcaattt atatattagt tatttatatt tggttcattt
    18001 gagttaacat atacattatt tactatattt agtatatttc tattaactta tatattgatt
    18061 actgtatttt aaattcttca attaatttat atactgctta ctaaatttaa aacattttaa
    18121 ttaatttgta cactgtttac tatacttgaa atacttctat taatctatat attgcctact
    18181 atacttaaga cattttaatt aatttataca ctgtttacta tacttgaaat acttc
//
LOCUS       DAYAOY010000089        17963 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYAOY010000089 DAYAOY010000000
VERSION     DAYAOY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.089"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..445
                     /locus_tag="ACGI6B_002985"
     CDS             <1..445
                     /locus_tag="ACGI6B_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9752402.1"
                     /translation="GSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIE
                     DLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGY
                     KNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            877..1131
                     /locus_tag="ACGI6B_002986"
     CDS             877..1131
                     /locus_tag="ACGI6B_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752403.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1167..1490
                     /locus_tag="ACGI6B_002987"
     CDS             1167..1490
                     /locus_tag="ACGI6B_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFG9752404.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1764..1916
                     /locus_tag="ACGI6B_002988"
     CDS             1764..1916
                     /locus_tag="ACGI6B_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752405.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2185..3033
                     /locus_tag="ACGI6B_002989"
     CDS             2185..3033
                     /locus_tag="ACGI6B_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9752406.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLCATWEDKSLIADNKVETVRAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQEEYNKK
                     KKELLE"
     repeat_region   3300..4050
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3300..3328
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4205..4393
                     /gene="srl"
                     /locus_tag="ACGI6B_002990"
     CDS             4205..4393
                     /gene="srl"
                     /locus_tag="ACGI6B_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HFG9752407.1"
                     /translation="MNKIATKSRAEYMKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4513..4878
                     /locus_tag="ACGI6B_002991"
     CDS             4513..4878
                     /locus_tag="ACGI6B_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9752408.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIIL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQDWISDEV
                     LPSIRQTGAYITNNVIPKS"
     gene            4931..5326
                     /locus_tag="ACGI6B_002992"
     CDS             4931..5326
                     /locus_tag="ACGI6B_002992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752409.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAEIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5378..5539
                     /locus_tag="ACGI6B_002993"
     CDS             5378..5539
                     /locus_tag="ACGI6B_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752410.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5867..6331)
                     /locus_tag="ACGI6B_002994"
     CDS             complement(5867..6331)
                     /locus_tag="ACGI6B_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752411.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6467..6784)
                     /locus_tag="ACGI6B_002995"
     CDS             complement(6467..6784)
                     /locus_tag="ACGI6B_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752412.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7067..7363)
                     /locus_tag="ACGI6B_002996"
     CDS             complement(7067..7363)
                     /locus_tag="ACGI6B_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752413.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7382..7795)
                     /locus_tag="ACGI6B_002997"
     CDS             complement(7382..7795)
                     /locus_tag="ACGI6B_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752414.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7779..8012)
                     /locus_tag="ACGI6B_002998"
     CDS             complement(7779..8012)
                     /locus_tag="ACGI6B_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752415.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8231..8605
                     /locus_tag="ACGI6B_002999"
     CDS             8231..8605
                     /locus_tag="ACGI6B_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752416.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            9217..9909
                     /locus_tag="ACGI6B_003000"
     CDS             9217..9909
                     /locus_tag="ACGI6B_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752417.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10105..12165
                     /locus_tag="ACGI6B_003001"
     CDS             10105..12165
                     /locus_tag="ACGI6B_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9752418.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12370..12693)
                     /locus_tag="ACGI6B_003002"
     CDS             complement(12370..12693)
                     /locus_tag="ACGI6B_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFG9752419.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12686..13387)
                     /locus_tag="ACGI6B_003003"
     CDS             complement(12686..13387)
                     /locus_tag="ACGI6B_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFG9752420.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13665..15050)
                     /locus_tag="ACGI6B_003004"
     CDS             complement(13665..15050)
                     /locus_tag="ACGI6B_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9752421.1"
                     /translation="MPINSFENYPMNWKRKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15158..16396)
                     /locus_tag="ACGI6B_003005"
     CDS             complement(15158..16396)
                     /locus_tag="ACGI6B_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9752422.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(16409..16786)
                     /locus_tag="ACGI6B_003006"
     CDS             complement(16409..16786)
                     /locus_tag="ACGI6B_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752423.1"
                     /translation="MIILKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCGNEISDDDIKELEQWLKKRK"
     gene            complement(17305..17727)
                     /locus_tag="ACGI6B_003007"
     CDS             complement(17305..17727)
                     /locus_tag="ACGI6B_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFG9752424.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         7012 a         1981 c         2629 g         6341 t
ORIGIN      
        1 cggtagttta aatagaaata aagcaagatt aaaagttgca agacttcaag aacatatagc
       61 taatcaaaga aatgattttc tacaaaaact atctactaaa ttaataaaag agaacgatat
      121 tatttgcata gaagatttac aagtaaagaa tatgattagg aatcgtaaac tatctcgctc
      181 aatttctgat gtgtcatggt cagagtttat tcgtcaatta gaatacaagg ctaattggca
      241 tggtagacaa attgtaaagg tgggtaaatt ctttgcaagt tcacaaatat gcaataaatg
      301 tgggtataaa aacgaggaag tgaaggactt aaatataaga gaatggattt atcctagttg
      361 taatgaaact cacgatagag atataaatgc aagtataaat atattaaaag aaggattaag
      421 actaataaca attcaaaata aataacgtat gtaagaaccg taggaactac ggggatagcc
      481 tgtggagaat tagtaagaca tatgttagta tgcaaaattc tgtgaagcag gaaacctgtg
      541 actttagtca tgggaggttc aggctagtat tactcattat tgtattcaaa agctaaagtg
      601 gaaacctagt gaatatatga atttagaagt taatgagaga gcattagcag ccgactcaat
      661 acttattaaa attgaagatg aagaagaaac aatgaaagaa gctgaaagag agaaaaagag
      721 aggtagaaga aggtagaatt taaagttaaa atagttacat tgctaatata tgaaaatgtt
      781 acaaatttgg aaatagatgg atatatatgt tgtatagtat aatgtagata atatatcacc
      841 aagtaaaaaa caacaaataa atctaaatta tgaggggtgg aaaataaaga tattaaactt
      901 acaatatgta tatttgcttt tgtaattgga ataatagtgt tattttgctg tataggtcta
      961 ggtcaattta atatggattg tattataaaa caaaatggtg ggtcaatagc taccgaacaa
     1021 tacaacatat atttagaaca atctatttca caatatagaa attttggttc tatactggtt
     1081 ttactaggag gaataggaat actgtttgat aaaagtgtca gaaaatacta aaaaagtata
     1141 tttttattta ggatgtgatg aatgatatgg caagagatgt atttagtgta aagatgacac
     1201 cactagaagc acagaaaatg gtagaaaaaa atttggaagc agatttagtt tctgttgata
     1261 tttataacct agaaaataat aagtctataa tagttacaat ctttgaaaaa tattatgcaa
     1321 gaagcaatag tgatgcaggt ttggtagtga tttgtgaaaa tactacagga aaaacacttg
     1381 ttaaaattac ctcaactggt agtgcaattg gactatttca gattgattgg ggaacaggta
     1441 ataatttgat taaaagagta aaaaaaatac ttattgatta tattatttaa ttattttatg
     1501 tagatattct taggttggta tggaatttgt actaacctat taagtaatca ataaaactaa
     1561 ataaacatag aaagcactta caaatatgta ggtgcttttt attgaaaaaa tagaaaataa
     1621 aataaaaagt aagatatagg aaaaatatgt aagaattata tgttataata attgtagcaa
     1681 gaagatgtaa tctacaattt atagagtgga gttcatgtat taaaaaatta tcctcccaac
     1741 gataagaagg gaggtgaata tacatggata attttttatt taatgtgtta gctagtgtaa
     1801 cagctagtta catagtttat ttgattagca agttaatcaa aaaagtaaaa agccactcta
     1861 ccgcaaagag tgacttaaag attgatataa attttaaata tcatcgtaaa aaataacttt
     1921 cagttataga acttcactct accgcaaata gattgtagtt cttcttgttt ttattatacc
     1981 acaaattaga aaaaatattg catataatat tttttatagt caataaaaat atgaaatttt
     2041 ttatacacaa tgatgatgaa tctaactaga tgaataaatt taattgtaaa taagaaagta
     2101 cttacttaaa tggtaggtgc ttttgttttg ccaaattatg ctataatatt gagtgagatg
     2161 agtaatttgt gggggaattt tataatgtgg atattagtac caataataac aatagtatta
     2221 cttataatag ctgtttcttc tatgcaatat attttagtta tgatagcttt tttgttgatt
     2281 atatattctt ttatagaaaa gaaaatagtt atgggtcttg tgtcagttct ttttttcact
     2341 tattcaattt atctttgtgc aacttgggaa gataaatcct tgatagcaga taataaagtt
     2401 gagactgtta gggcacaaag agaaactgta gaaagagaaa aagaaatgga aagaagaaga
     2461 atacaagaag aagtagacaa agaaagatat attgaaaaac atggaatgga aatatcagaa
     2521 aatgatttaa aagttaagct agaggcttta gtacctcaag aatataaggg gaagaaatat
     2581 gaattaaaag taggaaaatt taaaagatat agtatgtatt ttgatttaac tgtacaaaat
     2641 gaaaaattta gtaattctga agaatgtaaa aaatttgtaa aagaaattgc taatgatctt
     2701 aaaaaaatta aaataagcaa ggcgtacttt aagtttcatt caaaagatga cggcggtata
     2761 tataattctg tttatattga ttattttaga aacatacaaa ataatgtaga taatgttgaa
     2821 aatttagaat tcaatgagtt tgaacttaaa accgaagaag aagagaagcg agaacaagag
     2881 aaaatagaac aagaaaaaaa cagttataat aactacattc aaaacagagt ggttgaccca
     2941 ctagatagaa taaagaaact aaaagagttg ctagattcag gagcaattac acaggaagaa
     3001 tataataaaa agaaaaaaga attattagaa tagataatat agtaagcact tacaagtatg
     3061 taggtgcttt taaatttaca aagtattcca ttttaatttt atagtttaga ttttatgata
     3121 taataaaaat atagaagttt tgcagtgtgc gatatttgtt acaaagtagg gcttaatact
     3181 tgaaatctaa gatgttgagg gtgcgtgata agtgttatca attgcactat tgcccgctca
     3241 ctgcaatttt aagagtattg tatatatgta ggtattggaa atgctaagtt tattttgggg
     3301 ttttagatta actatatgga atgtaaatat tacatgggta aaaattttga tgatttttat
     3361 gacagtttta gattaactat atggaatgta aatattacat ttaatttgca ttgctttgat
     3421 gacttctttg ttttagatta actatatgga atgtaaatgc gtacaaggtt gagaagctcc
     3481 gcaatctcaa cgtcgtgttt tagattaact atatggaatg taaatatttg aaaggaataa
     3541 attttaaatg aatgaaagga taggttttag attaactata tggaatgtaa atcagccaaa
     3601 aacttcgaat ttgaactaaa atgcgtgggt tttagattaa ctatatggaa tgtaaattct
     3661 catgactatt gaataaatat aataaacaag catacgtttt agattaacta tatggaatgt
     3721 aaatttagaa tcaaaagcac ttaatcctag ttctttcata gttttagatt aactatatgg
     3781 aatgtaaata gctttttagc ttcgtctact actttataag caaagcgttt tagattaact
     3841 atatggaatg taaatgatga acaataaaac aatcatctaa agacgaagaa agttttagat
     3901 taactatatg gaatgtaaat gttcctgttg tgttttttac taatcccatt ttatatgttt
     3961 tagattaact atatggaatg taaattgctg tgatttcact aataaaacaa ttaataattg
     4021 tgttttagat taactatgtg gattcaaaat taaataaaca aagaaagcac ttacaaatat
     4081 gtaggtgctt ttattctgct caaaattggt cggttgggta aaatcattag aaaaaattag
     4141 taaaaacctc ttttctgtaa ctcgttacaa tattattatt aatgtaacga gttacagaaa
     4201 agaggtgaat aaaatagcaa ctaaaagtag ggcagagtat atgaaaaatt gtcgaaaaga
     4261 taaaagaggt tttagtgtac ttttagacaa agaaaagtta gataaatttg atgaagtatt
     4321 agaggaaaag aatctaacca agaaagaatg gctagaagaa aaaatcgacg aggaactgga
     4381 acaaaaggaa taaaaaataa gggtcactcc caccgaccaa agtttgagta acccctatga
     4441 tgtatactat cgtatatcaa ttataatata tgtcattcct taaaaaaatc aattattaag
     4501 gagtgtaata ttatgaaaaa tgaattaatg atgtttgaag gaaaagagat tgaagtattt
     4561 gaatttgaag gaagaatttt attcaactca aaagatgtag caaattgttt agacataaaa
     4621 aatgttaatg agaatataat acttatgaat gaaaagcaag ttgtaaagtt aagaaattcg
     4681 gatatcagta atactgacat ccgaaaactt aataatgctg gagaaaattt tcttactgaa
     4741 agtggagtgt ataaattaat ctttaaatca agaaaagaag aagctgagag attccaagac
     4801 tggataagcg atgaagtttt accaagcata agacaaactg gtgcatacat aactaataat
     4861 gtaatcccga aaagttgaga gaaaaagcaa gtgagattga aaaattacag ttgcttataa
     4921 cagtacatct atgttaaaag aattgctaga tgatgcaggc tttgacaata aatcaaaact
     4981 attaacagct aaaacattat ataagaaagc agaaattgat ttaccaatag agataaacga
     5041 agaagaacat tattttgata caaaacaaat agcatctaaa ctgaaaatat attctaagag
     5101 taataaacca gctcagatgg ctgtttgtga gattattaaa aagattgatt tagaagatgg
     5161 tgaagttaaa ggtgtttggg aaattaatgg ttcttggact ggtactgtaa ataaatatac
     5221 aaagagtgta atagataagg tgagaacttg gatagaggaa aataatagac ctaccaagat
     5281 tgcaggtgag aagaagaatt attatgtatt ttacaaaatt gagtaaattt atcagttgta
     5341 ttaaataatt tagtttagtt ttggggggat taatacaatg tgtgagaatt tacttgataa
     5401 tatgtatatt gaaaagagaa aagaagaata tagaattaaa cttttgaaaa taagagaaac
     5461 agatatagat atatataata agttagaaag tatagtatat aaactttctg agaaaaaatt
     5521 agagaaaaat aataaataga catataaagc acttggatat tctactgttt caagtgcttg
     5581 tttggtataa aatggtataa tggagataga gaattatatt aaccaaaaaa tggagaaaat
     5641 agagaagatg atttagttga cgctttatca gtaggagtac ctgcaggtaa gacagaatct
     5701 ctagtacgat tagttggaga tggaataagt gatactcaaa aaagtttaat aaataaaaga
     5761 gaatttagaa atataactca aattggtgga aatggaaatg agaaagcatt tagtgaagta
     5821 gaaaacttag ttaaatagat tagaaggtag caactagagt tggttgttac cttctttttt
     5881 tatactttct ctctctaagt attttatttt tcatatcttc taatacatct aatgcagtag
     5941 tataaaattg tatattattt ctataaggta tagctattgg aagaatgccc ttgttaacta
     6001 aaaaaataag aagtacaata aataaaagtg tcataatcat tctaataaaa taattagtga
     6061 tatctgataa atcaaatgtt aagttaattg ttgtagaaaa tacaacagcc ataatagata
     6121 tatacaaagc aaaaatgggg ttactatttt tttgtaactg tcctttaagc ctagattgct
     6181 ctatttctat atcttgaata ctttttatgg aatccatttc actataatac ttataaactt
     6241 ttaaataata ttttttatac ttgtttaaag cttcttcatt atctttaagg tctttgtcac
     6301 ttaactctga tttaacttcc ccaaaatcca taaaatccct cctacaatgt tatataatat
     6361 actttaattt atagcatatt taagaactta tatcagcaat ttgtcgaacg attttagcta
     6421 aaaatagagt ataatgatag gcttattatt tataagatat tattttttat atagttatat
     6481 atttctttct aattagataa tataatgctg ttgttataat ggctactaat attattgcta
     6541 ttattgtaat atctaatctt cttgtaaata agcctattgc tatatactca agacctgtta
     6601 catagcgata aagtacttct agtttaacag tttttttctt atctatattt ccatatattg
     6661 attctaattt attaaaatat tttttactac aaaaagcagt tttaggttta attaaatata
     6721 ttattgtagt tcctattata actattattc caataagtat aagattgata tttccaaaat
     6781 ccatagcaga tacctcctat taaatttgct tttcttaatc aaagtatatc agtaacacaa
     6841 gtacaaagag tatctaaaat attacaaata ttaacaggat agataaaact atagtaaaaa
     6901 ttgtaataat aaagcgaacg aaaattatca tactaaaaca taagacatgt tataattgta
     6961 ttagatagaa gattaacata caccaaagca ctccttataa taaagagtgc ttattttttt
     7021 gaaattcatc aatatataaa ctatcaagaa ccttattcaa cacaccttaa aatcgattta
     7081 aatagttctt tttgataaca taatttatat gatataataa aaaaatagaa gtgtaccacc
     7141 aatacacttc tatagctata actaagcatt ctcttgctgg gggagtgctt ttttcatttc
     7201 tccaaacaac attatttact tcatcagtaa ttatcaaatc aatctcatta tctttcaaaa
     7261 tctctgttgc agtacaaatt agattatcta agtcaaaact tcgagttatc tctagttgtt
     7321 tgttatttac ttctcttttt agtatataag ttttaaaata catttatatc accccttgta
     7381 tttactcttt tttctctaaa aatatattga taagtaaata tgcaacaaat aaaaaaatct
     7441 caatgacaca tagtgttata gctataacta gaagtgattg atttttccat gcactataag
     7501 ctatgatgaa aagaaatgta aaagtaaata atgtataatt cataatttta actaagatat
     7561 gacttgtttt cattgttaag tacaagtctc tttcatctgt actatcgtgt aattcttcaa
     7621 caatgccttt tcttgaaaaa gcattacaaa aattaaagat tgctattaca attaaaagaa
     7681 atgatgaaat taggaatctt ttatctttta aaatgcaata aataactaaa ctagcacaac
     7741 ataacacaca aactataata cccactataa aacttcgttt actctttact ttcatgtttt
     7801 ttatcctcca tttctttatt ttcttttaaa caacatagtt cttcaatcgt tgtaccaaac
     7861 acttgtgcca tacgataggc aagcataaga gaagggctat attgctcttt ttcaatagaa
     7921 ataattgttc tgtttgatac atggactaaa tcagcgagtt gttgctgtgt tagacccata
     7981 tttgttcgaa attctcgtac ttttgttttc attccataac ctctttctat atgaagctaa
     8041 cttcatgtga agttaacttc ataatatcct aaaatgagta aaatgtcaat ctcattagat
     8101 aagtattata aaacctaaaa gaataatatg gtaaaaattg gatggaatta tttaccttaa
     8161 ttctcttgtt tgtaaaactt tattgatata gaatttaaca tatacataaa tcggcaaagg
     8221 ggcgcgaatt atgaaaatta gtaaattacc agaagcggaa ttaaaagtta tgagatatat
     8281 ttgtgaagct aacaaggtat taacttctag ggaattagtt gctgcaatgg aagaaaaata
     8341 tgagtggaat gaatcaacta catttacagt tttgaaaaga ttagagcgaa gagagttttt
     8401 aagtacagag aaaataggca aacttacaca ttataatata ctagtaaaag agaaaaaata
     8461 tctaaggttt gaaacaaagg aatttttgaa gaacatacat aaaaattcaa tttcaagttt
     8521 aatgtcagcg ttacatggtg aagacgacga agtggatgaa gataaattaa gtgaactcga
     8581 agaatgtttt aagaatttga aataagaaaa tgacaaattg atacataata aataaaatga
     8641 aggtatgaaa ttataacttt ttctaccttc attttgttta ttattctgaa catgttaaga
     8701 aaatgaaata agtttgtatt ttagttatat aaattttaat agaatatgta cagtatatag
     8761 tagatagaat gtataaaaat agaatttaaa taagtagcta ttattgcagt gaaatgaatg
     8821 tttctaagta tatttgggtg gaagaatggt ggaagaaaat tagtgaaatg taattcttcc
     8881 accattcttc cacctagaaa atagaaactg tttaataaga attaatatac attaattcag
     8941 ctgaatgcaa tcaaaatact taaatgaaag taatctgaaa acaacataat tcaaatcaat
     9001 ttattcaaat aaacctcaat ttatcttaaa tcttaagaaa tatttaagaa ttaggataat
     9061 attttcttaa gatttatatt ttattatata gttataaggg aaagttacaa aaatgtaagc
     9121 caaagtaata taacaattta gtcattgttg agaaagggag tgaaagatat tgtataatat
     9181 agataagtat tattatacaa tagaggaagt gaaaatatgt acaatatatt agtagtagac
     9241 gatgataaag aaattgttga ttcgatagaa atatatctta aatcagaagg atttaaaata
     9301 tataaagcat atgatggaat gaaagctttg gaaataataa cagagaatga cattcatcta
     9361 atactaatgg atataatgat gcctaaatta gatggaatca aagcaactgt aaaaatacga
     9421 gaagaaagaa atattccaat aattttaatt tcagcaaaaa gtgaagatac agataaaata
     9481 ataggattaa atattggagc tgatgattat ataacaaagc catttaatct attagagctt
     9541 atagctaggg ttaaatcaaa cttaagacga tatgtaactt taggtaatta tgaagataca
     9601 tgtaaagaaa ttctaaaaag tggagctttg gagttaaata cactaacaaa agaagttaaa
     9661 attgatggtg aaaacataaa aatgactcca attgagtata aaataattga acttctttta
     9721 gaaaataaag gacgagtttt ctcaatagat gagatatacg aaaaagtatg gaatgaggaa
     9781 agttttaatt cagaaaatac agtagcagta catatcagac gaataagaga aaagattgaa
     9841 ataaatccaa aagaaccaag atttttaaag gtggtgtggg gagttggtta taagattgaa
     9901 aaattttagt ggaaaaatta taaaagaaaa gagtttaata tttaattgca ttttgatttt
     9961 agtcattttt acatttacac aaatgttagg aattttaata tgttttaatt ttttctctga
    10021 gagtttaatt tactctatgg ggattatgct tttcttaaac tttattttat ataaaatact
    10081 gacaatgtag atgaggtggg caacttggag aaatatagag tattaaagtt agtttcatta
    10141 atcataatat ctatctcatt tccattaact atattgttgg tgacaatagg acttgctgag
    10201 cctaaataca atatacaaaa aacttctaca aagagtggga tttttgatat atctgatgat
    10261 ttttatgata gtaatatatt tgacgaagga acaatggcaa gtctattacc caagtttaag
    10321 ctatctgtag gtggtaaatt aactagtaaa gaaaaaggta aattccaaca agaaggtaaa
    10381 agtatagatt taagtagtag tgattctgag tatgataaaa tcatgattaa tgaacaagct
    10441 tctgaagaat atttaagtta tctaaaaaat ataaaagtgt atgttattaa tacatacaca
    10501 ggagtatttt atacaaatac gtcatatgct agtttatatg attttaaaca aaaaactaag
    10561 agctattgta atgtagagat aataaataag ataggaaaga gctcatatat taaaacaata
    10621 aatggagaaa aatttgaact gaataatctg agtctggaac taaatgaagt agatgaaggt
    10681 tttgaggcat atgtatcttt tccagaagag ccaactatag aagatggaat agtatacact
    10741 aattttcaaa tatttaaaca agctacagaa aaagtaagat tattttttgt agctagttta
    10801 agtttagcaa ttatatgttt attatctata attttatata tcaaaataaa aggtgaagtt
    10861 ttaaaaagaa ataatgtatt tttactaata tacagtaaga tacctatgga atttaatgta
    10921 atattagtat ctatttcaat tgcaatttta actatatatg ctcttgctgt atcttatcat
    10981 attgatgtaa ttgcattagt ttctttatct ttactgatag tatcaaataa tatatttttt
    11041 ataaatgtgc aattgagcta tttagaaaat aaaatgtgta tatttaagac aagtattata
    11101 gtaagagtga ttttatggtt aataaaaact gtaagggagg tcagtaacgc tataaagaaa
    11161 gtatctttag caaggaaagt tattgttata gtgataatat ctttagcttt aatcatggca
    11221 attagcactc gaagtatagg tatgggatta ctgttttcta tatgtatttt tttaggattt
    11281 acaatatata taactaaaag actttcatat cttagttacg taatagaagg tacagaaaga
    11341 ataaaaaagg gagaattaga ttataagata aaaattactg ggaatgataa ttttacgtct
    11401 ttagctgaaa atataaataa tattggagaa ggtcttgata gagctataga agagcaagta
    11461 aaaagtgaaa gaatgaagtc tgaccttata acaaatgtat ctcatgattt gaaaactcca
    11521 cttacttcta taataaatta tgtagattta attaaaaaag aagaatcaat acaaccagag
    11581 tatataaatg attatataaa tgtacttgag tcaaagtcaa aaagactaaa attattgata
    11641 gaagatttat ttgaagcgag tagagtaagt agtggcaata tagaattaca aattagcaag
    11701 attgatttag tgcaactatt aagacaaagt ataggtgaac ttgaagaaaa attatctaaa
    11761 aataatttat atttaaaatt aaatgttcca aatgataagg tgtatatatg ggcagatgga
    11821 agaagaatgt atagagtatt tgaaaatctt ttatctaata tagcaaaata ttccttagaa
    11881 gaaactagag tctatattga tgtatatgac tatggtgaaa atgtaaaggt aactatgaaa
    11941 aatatatctt cttatgaatt aaattttgac ccaagtgaga ttatggaaag atttaaaaga
    12001 gctgatgaat caaggaatac tgaaggaagt ggattagggc ttgctattgc aagagattta
    12061 gtagctttac aaggtggaaa attttatata gaaatagatg gagatttatt taaagcaaat
    12121 ttagagtttc aaaaatatga agaagaaata acaaatatgg aatgagatag tatttaagta
    12181 tatatatttt tttactgtcg aaagctttta ttataatata ttagaatatt tacagtagag
    12241 aatgaagagt aaaaacagag taactttaag tcactctgtt ttttatttaa aatcaataat
    12301 ataacaactt aaattaaaat tgtacacata gttttaaaat aacttataca ataaaacatt
    12361 atccaaatat cacaagataa ttccaaataa atatatagat atcactcctg taataataga
    12421 aataccaata gatttaaact tatatgcaac aagaagtaca atcataaaag atataatttt
    12481 agggttagct atagataaat atagactatc tccattaaaa aatacatctt tagcagttaa
    12541 ggaagtaagt attgctacag gtataaattt catgattttt ttcaaaaaat caggcatctc
    12601 tttttttgaa aaaattaaca taggaagcac tcttggtaga tatgtaacaa tgtacattcc
    12661 aattataata aaaaatatat aattattatt cataagattc ctccttttta cagtaagatt
    12721 cttctataaa gaaaccaatt aaagatgcta aaattgatgc aactattatt actaaattgc
    12781 ttttgaataa tgcatataaa ataacagaaa taatgattga tgatataaat actacaatat
    12841 aaatcttatt ttttatttga gaaactatta aagctataaa catagaagtc aatatgaagc
    12901 cagaaataga actgtctata gatatacttt ctcctaaaaa tgctccaata aaattagcta
    12961 aaactgatga aattaagcag aagaaattaa gataaatagc atttctatca gaccaatttc
    13021 cagcagtaaa tttttcataa ttaactgcaa aagtttcatc tgttatagta tgacaaaatg
    13081 gcaatataaa ttttggactt ttatttttta tgtacatgtt caaattagaa ctcattagaa
    13141 aatgtcttaa gttaactata aatgtagtca aaacaataga tgttacactt gcaccagtag
    13201 aaatcattga agctgcaatg aattgagaac ttcctccaaa tactaaagtt accattagtg
    13261 ctatttgaaa aggtgaaaga ccagattttt gtaatagtat tcctcctact atacctatag
    13321 gtatataacc taagaatata ggaattgaag caataaagct ttcttttaat tgttgagttc
    13381 tttccacaat aaatcactcc ttttgtatta aatgtattaa agtattcgta aaacagaaaa
    13441 atagattaat tgtataattg caattataat taaaaataaa tttggattaa tatataaaat
    13501 actatacaac aaatagataa tcttaaacgg ttattaaatt ttatctattt gttattatga
    13561 tgtaataata catattaatc tctataagtt aattactatc tggtgtaatt ttattttaac
    13621 aacaaaaaaa aatacacaca attaaaatat tgtgtgcaat acaattattt tttctcttct
    13681 aatagtcttt ttaatacgct caaaccagct ttaagttctt ctgtactttc tgtagcacat
    13741 acagctattc ttattgcaga aattggtttt tcttttccaa cagcaaaacg ttcagaagca
    13801 tagacctgta caccttcttt aagtgcttgt atctcaaatt tttctgccat tattccatca
    13861 ggtagaatca accatctaaa tatacattct tcatttccca aaagattata tccagataaa
    13921 tattgattaa taatcttgtt tcttcttctt gcagatttac ggcgtgcatt aattaattta
    13981 tcggcttcac ctgatgtaat catgcgataa gcaatttctg ttaaaaaata agattgagat
    14041 agattaatgt tgtataatgc gcttgataat gcctctcgat actgtttagg tgtagagata
    14101 tatgctagcc gtaaactagg tgcaataatt tttgataaac tcgtaatata aattgtttga
    14161 ttgggcagat aggaagccac tggatttaaa tgtgtttcat taagtaaact atgaatagca
    14221 tcctcaataa caattaaatt gtatttagat gcaatatttg caatcatttt tcgtccattt
    14281 tgtgacatta tatgtgtagt tggattttga taatctggta ttatgtatag acctttaata
    14341 ttttcatttt tacaagcata taatagtcct tcttcactaa tctcattatc ttcttgttta
    14401 attggaatta gttgaatccc aagcatttta gctgctgtct ttattccagg ataagttaat
    14461 gggtctacac ctattctatc tccatgttga aacaaaccag ctaaaatagc aacaattgca
    14521 ttttgtccac cacttgcagg aagtaagcta tctgatgtag tttcaagccc agctttataa
    14581 attaatttgg caatagcttc tttttgccat ttagcaccat ctctttgtcc atattggaat
    14641 atggttttaa aatctatttc cttaattatg tgtttaaaca aagtattcat ttcttctagt
    14701 gtgaaatctg gattcattgt gcccatttca ataaatgtaa ttttattatt actggacatc
    14761 aagaatagat ttgaacgtgt atcataagat acaaaagttc cacttcctgt aactccactt
    14821 attagacctt ttttttcaca aattttaaaa gctctagaga tagtactcac attaacatct
    14881 aagaaatctg ccaattcacg ttggggtggt aatttagtac ctggtaataa aaatccattg
    14941 ttgatatctt gttctagttg ttctgcaagc gccttatata atatttgccc ttttgaaggt
    15001 ctctttcgtt tccagttcat aggataattt tcaaaagaat taattggcat aagttttcac
    15061 ctcacattat taatttgatt ttttctttat ctaaaacaat aaattctaaa gatacaaata
    15121 aaaactgata ggaaataatt ctatcagttt ttgtctttta attgatttca aatcttccta
    15181 taatttttat accttcttta tcataaagag ggattgtttt tttaaatata ttaaaatttc
    15241 tttttcttgt atataaaaca gcttcttctg gtgtctttgg ttgattatca caatcgtaca
    15301 aatctctttt ttttacgtag ccgacttttt tacctctacc tattgtttgt ataagttctg
    15361 gttcaatgga atttccattt gagtctatta tatgtgtacc ataggtttct ccatttttat
    15421 tataattaat cttaccaata gtatattcta catcttcatt ttgtttaaaa ttggagttga
    15481 tagaaaatat tgaccctatt attataaagg ttaataaaaa cttaagacct aaattaattt
    15541 tctttttatc ttgattaaac atagaatcta acctcttttt taaaataata ttttttttat
    15601 ttagaaactg agatacgaat gatgaatact tcaaagattt ttgatacttt attgtatcta
    15661 atattatata tgcatactct ttaatttcac tcgtggtaca cttgtcaatc acaatttcat
    15721 cacaagataa ttcacataat tcatgaaaat atttttttaa taagtataaa attggattga
    15781 accaatatat gatacaaatt atactaatta gaagcttaaa taagttatct tttcttttat
    15841 aatgaattaa ttcatgttta aagatgaatc ttaactcttt attattgtac tttttttctg
    15901 gaattattat tttacagcgt gttaaaccta taatggcagg agaataaata cctttaacct
    15961 tatatatctg aagtttttta tttatactta gttcttttgc ttgttcatct aagatactag
    16021 taatatctat ttctaaaatt tcttttttaa aattttctat gtaattataa aatttaaaat
    16081 ttgttcttaa atagtaaaaa gagattgcta taattcctaa tatccagatg tacataaata
    16141 tatctaaagt aatgccctgg tcatatggta tataattatc gatattatgc ttatagatat
    16201 taatagtagt attattgata tttaatatat ttaaatttat tttagatata aacatcattc
    16261 tgactactat taaaagacat ataaaataat taaacttaaa agtatatttg cttatctttt
    16321 tagtaaataa aatgaaaaaa agaattagtg gacttatgta aatattaaat ttaataactt
    16381 ctataaataa ttccataaaa tacgtctcct attttctttt ttttagccat tgttcaagtt
    16441 cttttatgtc atcatctgat atttcatttc cacaacctaa tgatgatata aagctatgaa
    16501 tagaattttt atgtataagc tctaaaaaat cctttgtttc aaattttaga tattcctcat
    16561 gtttaacttt aatagtgtat aaagtatttc taccatcttt ttcaaattct aaaaaacctt
    16621 tatcacttaa tctagaaagt actttaccag tagtaccttt tgtccatcca tgagaaattt
    16681 ccatataatt agctacttct ctggctggta ttttatctat gtctttatcc catatgaaat
    16741 tcataactaa caactcggat tttggtattt ttttcaatat aatcactcac ctttaacttg
    16801 attatattag acaaatgtct aatagtcaat ataatatatc atcatgtata aataatcttt
    16861 atattatttt atgcttgaga actaagtagt atagatttat agataaaaaa gatattgaga
    16921 gttttatgga agaggggaat atattttcta ttagagataa gaatatgaca tatatttttt
    16981 attgattttt acaaaaatac tatatagtga ataaattaat atcttttcta aattagaaga
    17041 ggagaatctt attagataaa gattatcctc tttattgaaa gtttaaggga tattagagat
    17101 tattaatttt tctctctttt tttaactaaa agcaataata cataaaatta ttattgctaa
    17161 tgatactctt aatgtggtat atagtcatag tgtcacatgt ttatcaatag ttttttcatt
    17221 aattattgta acgaatagta ggactcataa aatacttaga aatatgtttt tagatagatt
    17281 ttaaatatat aatctaactt atatctataa attaacacta ttattctcca attccatttc
    17341 tagttttttt ataaatttat taaattcttc tggttgcctt tttccaaaca atactcttac
    17401 aagagctgaa ttgatttttc caaatatatt cctcttgctc tcactttcaa taaaaataag
    17461 ttttgtattt tctaagtcaa tatgtactaa ttcatatcta gtaatagtac tttctctttt
    17521 attgcttgtt tttatctcat atactctatt tttttcgaaa ttagttattt caaccttaaa
    17581 tcttttatta tttttttgct gtacaaatgt tcctactgct tttttctcat taaaattagg
    17641 aaaatttagt cttgcagatt ttgtaaatat attaaatatg tcttttgcag aatattttaa
    17701 taaaacttca gttttaaatg tattcatatc gttttccctt ctgtatataa atttaatatt
    17761 tataagttaa tttttaagtt aaaatagtat aatatgccat attaaatcat gttaaaacta
    17821 tttatatagc taaaatctta cctatataag tatgtatact atcatataaa aataaaatct
    17881 taaaatagaa agggttaaaa acacagttta ttataatttt tattgttgag tagatacgta
    17941 ctattatata tgattgagca agt
//
LOCUS       DAYAOY010000090        17645 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYAOY010000090 DAYAOY010000000
VERSION     DAYAOY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.090"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            90..878
                     /locus_tag="ACGI6B_003008"
     CDS             90..878
                     /locus_tag="ACGI6B_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HFG9752425.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            899..1639
                     /locus_tag="ACGI6B_003009"
     CDS             899..1639
                     /locus_tag="ACGI6B_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFG9752426.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            1815..2189
                     /locus_tag="ACGI6B_003010"
     CDS             1815..2189
                     /locus_tag="ACGI6B_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752427.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            2281..3741
                     /locus_tag="ACGI6B_003011"
     CDS             2281..3741
                     /locus_tag="ACGI6B_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFG9752428.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            3794..6823
                     /locus_tag="ACGI6B_003012"
     CDS             3794..6823
                     /locus_tag="ACGI6B_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9752429.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            6900..8966
                     /locus_tag="ACGI6B_003013"
     CDS             6900..8966
                     /locus_tag="ACGI6B_003013"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9752430.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    9684..9894
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10120..10935
                     /locus_tag="ACGI6B_003014"
     CDS             10120..10935
                     /locus_tag="ACGI6B_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9752431.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            10963..11640
                     /locus_tag="ACGI6B_003015"
     CDS             10963..11640
                     /locus_tag="ACGI6B_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFG9752432.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            11699..12421
                     /locus_tag="ACGI6B_003016"
     CDS             11699..12421
                     /locus_tag="ACGI6B_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFG9752433.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            13068..14219
                     /locus_tag="ACGI6B_003017"
     CDS             13068..14219
                     /locus_tag="ACGI6B_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFG9752434.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            14265..15440
                     /gene="thiI"
                     /locus_tag="ACGI6B_003018"
     CDS             14265..15440
                     /gene="thiI"
                     /locus_tag="ACGI6B_003018"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFG9752435.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            15596..16399
                     /locus_tag="ACGI6B_003019"
     CDS             15596..16399
                     /locus_tag="ACGI6B_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9752436.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            16642..17283
                     /locus_tag="ACGI6B_003020"
     CDS             16642..17283
                     /locus_tag="ACGI6B_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HFG9752437.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         7148 a         1682 c         3236 g         5579 t
ORIGIN      
        1 caattattaa aggctatttt tattaaagat agtcttttta taatgtcata aatgattaga
       61 tatgtataac taaagaaagg agggaataga tgacaacttt aatttttttc atagtgggga
      121 tagcatcttt ggtagttgct tttgttattg atgggggaca tgttggagca ttatttgtat
      181 tttcatcagc acttgttgtg tttggtggaa ctataggagc tgttggagct tcgactccat
      241 tccctgtgtt taaaaagtct ataaagataa tgttattagc ttttaaaaat aaaaagccta
      301 ataacataga aaatataatc tatttcaaag aaattgcaac aaaggctagg aaagaggggc
      361 ttcttagtat tgacaaagaa ataagtgaga atgatgaatt agaccctttt attagaaaag
      421 ggcttgaact tatggtagat ggagtggatg cagttactat aagaaatata ttggaatcac
      481 agatacagat aacatcaaaa cgacataaag aaggaatagc aatttttgat tcagcaggtg
      541 gatatgcacc tacactgggg ataataggta cagttatggg attagtacat attttaggta
      601 atctttcaag taatccagat gcattaggac ctcaaatttc tgttgcattc ttggctactt
      661 tgtatggtgt tggttctgcc aatttaatat ggttaccaat agcatcaaaa cttaaggcta
      721 taaatcaaca agaagtaagt gaaagacttt tggtgataga agcaatatta tccatacttg
      781 agggtgataa ttctaattta atgacagaaa aattaaaaat atttttaaat tcaaaagaac
      841 taattgaact tgaagaaatt ggtggtagag ctgaataata taaaaggatg gaatgtttat
      901 gttgtatgat gaagatgaaa aagaagaaaa caatgagcga tggcttttaa cttattcaga
      961 cctcataaca ttacttatga ttttctttgt aataatgtat tctatgagta atgttgatgc
     1021 ggagaaatat aaacaattat ctcaatctct aaattctgct tttggtggtt cttctggagt
     1081 tattgaaggt ggtaatagca agatagaacc agtagttgaa ccaggaagta atgatttaga
     1141 tactttacaa aatgctaagt ttaaaaaaat tggtgaagaa attcagaagt atttaaacga
     1201 aaatggtatg gctaattcag tatcattaag agttcaagat agaggattag taataagcct
     1261 taaagataca atactatttg atacagggaa agctattgtg aaggataatt ctagagataa
     1321 gattattcaa ataggtaaga tgttaaatga aatgaatagt tacatgagag tagaaggcca
     1381 tacagacaat atgtctataa aaaattcaga gtttaagtca aattgggatt tatcagttat
     1441 gagagcaacc aatgttgttc aacttcttat tgacaatgca ggtatagcac cagataaatt
     1501 atctgctgta ggatatggtg agtttagacc aatagcagaa aattcatctg aagaaggtag
     1561 gtcaaaaaat agaagagttg atatagtttt agttgatagt aaatatgata atgtagaaaa
     1621 tgtttcaaag gataaatagt taaattagct gtctcaatac agaattaatt ctaaaggaga
     1681 tggctttttt atgcgaaaat caaaaataga tacatagtaa gttacctttt tgtaggaata
     1741 tatggtatct taaatatata atatttaaat aatgtataag ctcaattaaa aaatttatag
     1801 aggatggaaa ataaatggga aataaaaata aaaatactaa atataaagat aaaacaagag
     1861 aaaggtataa taaaaaagtt gagaatttaa ataaagaaaa attaaataaa attaataagc
     1921 aatctaagaa agtagataag attgatgata ttattaataa atctagtatt gtattatgtg
     1981 tacttctagt agtgttttca ttgctttact tatttaaact ggttcaactt aatataataa
     2041 tgatactttt agtaattaca gtagctattc aacaaggatt ggtgtcattt ggtgcattta
     2101 gaaataaaaa ttatgtaaaa gctttatttt ctatatttgt aatagtatgt ttcttaattg
     2161 cttttaaata tgttgtaaat cctgtttaaa tatatcaata aacgttctat aattaatata
     2221 aatagtgaag ttacagtttt aatgctatta taaattttat aatttgaagg agtagatttt
     2281 atgaataggt gtaagtttgc aaaattaatt atcacatcaa tgattgtttt attacttaca
     2341 ggatgtagtt taaagaagga agataattat aagttagacc cagaaaatcc gacatcaata
     2401 caaatttggc attattataa tggacagcag aaaatggctt ttgataagct tgttaaggaa
     2461 tttaatgaca ctgtaggtgc tgaaaaagga attattgttg aagcattcag tcaaggtaat
     2521 gtagttgatt tatcagagaa agttttagat tctgcaaata aaaaagttgg ttctgaagat
     2581 gtaccaaata tatttgcagc atactcagat actgcatatg aaatggataa attagggtta
     2641 ctagtagata tggataagta cataagtaaa gaagaattaa atgaatacat agacgaatat
     2701 gtagaagaag gcagaataaa ttctaaggat gagtttaaac ttttcccaat tgcaaaatca
     2761 actgaaattt tgatgttaaa taaaactgat tgggataaat ttgcaaaagc tacaaattca
     2821 aatataaatg atataaaaac aattgaagga ttagtagaga catcaaaaaa atattatgaa
     2881 tggacagata gtttaacgaa agagcctaat gatggtaaag cattttttgg aagagatgct
     2941 ttagcaaatt atattattat aggaagtaaa caactaggag tagaaatttt ttctgttaaa
     3001 gatggtaaag ttacgctaaa tattgatgaa aaagttatga aaaaactatg ggataatttc
     3061 tatgtaccat atataaatgg atattttgct tcttatggta agtttcgttc tgatgatgca
     3121 aaaacaggtg atatattagc tcttgtaggt tctagttcag gagcagcata ctttccagat
     3181 agagtaatgc taaatgataa tgagagttat aatattgaaa catctgtttt tcaagcacca
     3241 cgttttaaag gagaaggaaa atatgctgta caacaaggtg caggaatggc tgtaacaaaa
     3301 tctactaaag aagaagaata tgcatcagta gaatttttaa aatggtttac agataaagaa
     3361 cctaatataa aattctctat tgcttctgga tacttaccag taaagaaaaa aacgaatgat
     3421 ttggatgtta taaaagaagt tatgaagaaa gataatttga gtttgtctga taaagttgaa
     3481 ggttcaatag cctcatctat tgaacagatt aacagttgta atttatatac taataaagca
     3541 tttgatggtg gaactaaagc tagacaaata cttgaatatt caatgcttga taaagctaaa
     3601 gaagattcaa ataaaataaa tgaattagta aaaagtggaa cttctaagaa ggatgcagta
     3661 gcacaattta ataatgataa aaattttaaa acttggtttg aaagttttaa ggctgagctt
     3721 gaaaatgcta taaaaaaata gatgaattgg cagttagtgt gttgacgagc acaaaggtat
     3781 aggtggaatg ggtatggaaa acaatctaag aaaaaacaat tcaataatga aaaagatgat
     3841 atttccccta attattgtta tgctaattca aacaagttta ttttgtgcca caattttatg
     3901 gggaggaact attaagaaat taaatgataa ttcgtttgac atcttaaatg aaagagtaat
     3961 taatcgtaaa aattatatac aaaatgaaat gttacaaaga tggtcaaata caagtgaaac
     4021 ggaagctaca attaattcct ctgtaaaaaa agtattaaat gaaaaaaatg ccttaattga
     4081 ggatgtaggc aacaatgaag aaattaacaa agatattata aaaagcatat caaatgattt
     4141 aatatatctt ttgagaaaaa attctgttac aggagctttt ataatcctaa gtgacaaaaa
     4201 tattaacgat aagtcaatag atagtaaagg tgaaataagt aggacaggat tgtatataag
     4261 ggatttagac cctaagttta atccaaatga taattctgat ttgttaattg agagaggttc
     4321 atcagatata gcaagaagtt ttggaatatc tatggacagt tattgggaac ctaagttttc
     4381 atttaaaagt gaaaatgagc aagatggaga tgattttttt tataaaccat ttagagctgc
     4441 aattgataat ccaaatgtat caagttctaa tttaggatac tggggaagta agtttttatt
     4501 gacaaatgca ggtgataggc ttatgacata tacagttccc cttatttatg aagatggaac
     4561 tgtatatgga gtacttggaa tagatttaag tgtaaattat ttaagagaat tacttccata
     4621 ctcagagata aatgggaata aaaagggtaa ttatatttta ggtgtagata gaaataatga
     4681 tatggccttt gaaaatgtaa tttcaaatgg atactcatta aaagaaaaat tttgggatgg
     4741 tttacattct aatttagagc agaaggaatc gtataaaaat atatataaga ttaaaaataa
     4801 agataatgaa tgtggtaatg gcaacattta tgctagtgta caatacttag atttatataa
     4861 ctccaatact ccatttgaaa atgaacgttg ggctcttttg ggtattattg aagaaaaaag
     4921 tcttttgtca ttttcaaaaa gggtagagaa attagttata atatctatag caatttcttt
     4981 attaataggt ctaataggta tttatattgc atcaagatta tttacaaaac caattgttct
     5041 tttagccaac aaagttagaa acagtaatcc atcaaaacca gtaaatcttg ataagataaa
     5101 tatatctgaa atagatgaat tatcttcagc tatagagttg ctcagtgcaa atgttgcaga
     5161 ttcttcgtca aaattatcac agattattga aatggtagat atgccaattg gagcttttga
     5221 gttatacaaa aacgcagaga atgttttttg tacaaaagaa ttttttagcg tgtttactac
     5281 tgaaatcaaa tttgaagaat atggatatat ggaaaaaaat aagttcctga aaaaattaag
     5341 gggactagat aaatatttgc aagaaagtta tgaaacagaa aatacatata tatataagtt
     5401 tgaatctaaa gataaaaaac ttaagtggat aagaatgaat atctttgaag atgagctaaa
     5461 ggttcttggt gttgttgttg atgtaacaaa agaatttatt gagaaaagga agatagaata
     5521 tgaaagagat tttgatattt taacgaatct tttaaatagg agagcttttc attcacagat
     5581 acaagaaaaa tttaagaata aagatgaact taagatagca gcatttatta tgtgggattt
     5641 agataatcta aaatatatta atgatacata tggacatgat gctggtgatg aatatattag
     5701 gtgtgctgca gacatactta agaagtttat atcatggaat ggtatagtat ctagactttc
     5761 aggtgatgag ttttatgtat ttatatatgg atatgaaagt aaagatagta taagagatat
     5821 tattaattct gttaagaaac agatgaataa taccttatta aaacttcatg atggaacaga
     5881 atttaaaata agggcatcag caggaatttc ctggtaccct gatgactcaa ccaactatga
     5941 tgaattaatg agatacgcag attttgctat gtatcaaatt aaaaatacaa tcaagggtga
     6001 agttagtgaa tttgatatta atatatataa taaggattca ttcttattga gtaatagaga
     6061 agaattaaac aagttaattg atggtcaatt agttgagtat gcatttcagc ctattgtaga
     6121 ttgtaaaggt aaagtttttg catatgaagc acttatgaga cctaaaataa aaacttttag
     6181 ttcgccaata gatgtcatta gattagctcg ttcacagtct aaattatatc aaattgagcg
     6241 tattacatgg tttaaagcac ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg
     6301 caaactgttc ttaaattcta ttcctaataa taatttgtca aaagtagatt ttgaggaaat
     6361 tgaagattta tatggtgaat atttaaatag agtagtacta gaaataacag agaatgataa
     6421 gattaatgaa gattttattg agaaaaagaa agcatatata gaccattgga atatagaaat
     6481 tgctttagat gactttggga caggttacaa cggagatgct gtactattat atataatgcc
     6541 agactatgta aagattgata tgtctattgt aagaggtatt gataaagatg aaaacagaca
     6601 gaaaatatta aaaaatctta tctcatattc taacgaaaga aatattaaag tgatagcaga
     6661 aggtgtagaa acaaaaggtg aaatggaaac actaattaat cttggagtag attatatgca
     6721 aggctactat attagtaaac catcatttat acctgaagat ataagcgaca aaattaaaaa
     6781 agaactcaag atatgtcatg aaaataggaa tgatgtggtt taatagagga aaaattaaat
     6841 ttatttttat gaacttttag tttataatta aaaagaaaat aatataatta taaatcaagg
     6901 tggtggagct aataatcaca acaaatatag atataagaaa gttaaatgaa aatcaattag
     6961 aagcagttga gcatatagat gggccttgca tgatacttgc agggcctggt tcaggtaaga
     7021 ccagagtaat tacatataga atagccaata tggttgtaaa caaaaacata gcaccaacaa
     7081 gaatcttggc aataagtttt accaaggcat cttcaataga aatgaaaaat agagctttga
     7141 atttaagtga tgacataaga cttaataagg taacctatgg tacttttcac tctgtatttt
     7201 ttaaaattct aagatacttt gaaagataca acttagacag tatatttgat gaaaagtcaa
     7261 agcgaatgac aatcaaggca atcctaaaaa gtttaaatat agagaatgct gatgatgatg
     7321 agaatatagg acaagttata aatgaaattt cctatgtaaa aaatgaactt atggataaaa
     7381 atgaatttaa ttcagaagta ttaacaaaag atgagttttt aaaagtatac aacttatacg
     7441 aagaacaaaa atctaaagtt aataaaatag attttgatga tatgcttata agaacttatt
     7501 atttactttt gaacaataag tctgcactag aaatggttag aaatgtgtat aaatatatat
     7561 tgatagatga atttcaagat ataaataaag tacagtttga agttttaaag ttaatttgta
     7621 gcccattaaa caatatattt gcagttggag atgaagacca aagtatatat ggatttagag
     7681 gtgctagacc agattttttg cttgaatttg aacaatactt taataatact aaaaaaataa
     7741 tattagatat aaattacagg tctaaaagtg agatagttca tactgcaaat agacttatcg
     7801 acaaaaataa aaatagatat gaaaaaatca ttaagtgtag tcagggggat ggaggaagtg
     7861 ttacctatat ttctccgcat gattcagagg aagaagcctt atatattgcc agggaaataa
     7921 ttgacgaaat acaaaaagat tatgtagagt attcagattt tgcagtaatt tatagaacta
     7981 atatacagtc aagagcatta gtagatgtat ttatggatat gaggattcca tttgtagtaa
     8041 aagactcagt aattacaata tatgaccatt gggcaagtca agacatacta gcctatttaa
     8101 gaataggtat taatccaaaa tcaaataaag attggttgag aattataaac aagcctttta
     8161 gatatatatc caaagacagt ataaatatgg ttaaggatga aaaagacttt ataacagcat
     8221 taataaataa gtgtaatctt catcctaaac aagttaaaac aataaatgat ttggaaatag
     8281 atttaagtta tttaaatact ttaaatccta aaaatgcaat atcttatatt agaactagtc
     8341 ttgattatga tagatatgtt ttagattatt gtagcaacag aaagataaag actaatggac
     8401 ttgtagagat attaaatgaa cttgagagtt cagcaactaa ttttaatact gttacagagt
     8461 ttttagagca tattgataga gtaaagactg agttaacgga aaataacaag aataagcaga
     8521 ctgagggtgt catatttaca actatgcata gtgcaaaagg attagaattt agaaatgttt
     8581 atattatagg tgtaaatgaa ggaacaatac ctcatgaaaa atcatatgat atttgtaagg
     8641 aagagaaaaa agaagaacaa ttagaagaag aaagaagact tatgtatgtt gctattacaa
     8701 gagctgaaga aaagttatgt ataagctcta ctttaaataa gtatggtaag aaagttgata
     8761 aatcattatt tattaatgat ataaaatctc caacgaaaaa agaaattgac agtattggtg
     8821 taggggataa ggtataccat aaaaaatttc atgaaggaga aataattaaa aaagatggaa
     8881 ttatgtttac aataaagttt aaagatagag agcgaatctt ggatttgaaa acttgtttat
     8941 tgaagaatat aatttatact atttaattta gaatctaaat ttagatagaa atataatgta
     9001 ttatttatta tgtgcaagac aaatagatat gtgtaaaatg tattatttaa cttattagaa
     9061 aaagaaataa gggtttaata tattatttat agttgtttag aaaaagcaac aatgtggaaa
     9121 atattattta gtattattaa agaaaagcaa caatgtgaaa gatattattt agtgtaattt
     9181 aagaaaaagc aacaatgtga aagatattat ttagtattat taaagaaaga gaaacagtgt
     9241 gaaagatact atttagtatt attaaagaaa aagcaacagt gtgaaagata ttatttagtg
     9301 taatttaaga aaaagcaaca atgtgaaaga tattatttag tattattaaa gaaagggcaa
     9361 cagtgtgaaa gatactattt agtattatta aagaaaaagc aacagtgtga aagatattat
     9421 ttagtgtaat ttaagaaaag caacaatgtg aaagatatta tttagtattg tttaagaaaa
     9481 agcaacaatg tgaaagatat tatttagtat tatttaagaa atatctaaaa atatgaaata
     9541 tactatttat aattacaaaa ctaatatcaa taaatataaa cagtagtatt tagataattt
     9601 acaagtaaaa attattaaaa aaataaaaaa cttattgaca aaaatattat aaggaagtac
     9661 aattgtatta acaatttaag gaaacaaatc caatgataga gaaagtagca tatatggatt
     9721 tacagcgagt taggggttgg tgtaagccta gcaatgaagt ctatgtgaag agagctctgg
     9781 aggagattat cagaaacttt agtatgataa ttggtttacc cgccttaagg gattgagagg
     9841 ataagtactt acttatcaaa aagagtggta acgcggatat aattcgtctc ttagctgtaa
     9901 agctaaggga cttttttgat ttaaaaataa aagtatccat acagcttaaa tgctaacagt
     9961 cacaaatttt gaaagtaata taagtattta tcaataagag tggtaacgcg gtaaagtcgt
    10021 ctcttagtca ttaaagactg agagactttt tttgtaactc gaaacattta agtattaaaa
    10081 tctgagggaa attataatta aaagagaggg gtagttaaaa tgaaagtatt taaaaaatta
    10141 ttaagtttag gattagtatt aggattgaca ttatcgctag taggatgtag tggtggagga
    10201 gaaaaaacaa agttagaaca aataaaagac aatggaaaat tagttgttgg tactagtgca
    10261 gaatttcctc catttgaatt tcataaggta gtagatggaa aagattcaat aaaaggattc
    10321 gatataatgt tggctgaaga atttgcaaaa gagcttggtg taaaggttga aattaaggat
    10381 atgtcatttg atggattaat aggtgcttta aatgcagacc aagttgatat tgttcttgca
    10441 ggtatgtctc caacaccaga gagagaaaag agtgttgatt tttcagaatt atattattta
    10501 agtagaaatg cagttatagt taaagatgca gatatagaca aagtaaaaac tgaagatgat
    10561 ttaaagaaac ttagagttgg agttcaagca ggaagtatcc aagaagaata tgtagttaac
    10621 actttaaaaa tgacaactac aaaatctttg aaagcaatac ctgatttaat tacagaatta
    10681 aaaaatggaa acatagatgc agttgttact aatgaagcag tttctttaat aaatgttaaa
    10741 aaatatgatg gaataaaaat ggcaaatact gaagttggga aagatgtaac agaaggtatg
    10801 gcagcagcaa tcaaaaaatc tgacaataac aaagatttta tagaattatt aaacaaaaag
    10861 ataaaagaat tacaagatgg caagaagata gaagaatttt taaatgaagc atctactgaa
    10921 gctgcttcaa attagtaaga aagttatgtg ggagatgaaa ttatgagttt agattttagt
    10981 tttttaagta gatttgggac ttcttttttg gaaggaacag gtgtgacagt atcaatttct
    11041 cttgtggcat tatgctttgg atttataata ggtataatta tctgtatggc aaaaatatca
    11101 aagagcaagg tattaagagc aatatcatca atctatattg aggttttaag agggacacca
    11161 ttattagtac agatatatat agtatggttt ggtttacctc aattaggaat aagattccct
    11221 atgttgtttg gtataccaag tgaatttata gctagtgcat ttgcattatc agtaaactct
    11281 ggtgcatacg tggcagagat acttagatca ggtatacaat cagttgataa tggacaaatg
    11341 gaagcaagta gatcattagg attaaattat tggtcaacta tgagatatat aataatacct
    11401 caagctataa aaaatatatt accttcatta gcaaatgagt ttattacact tgtaaaagaa
    11461 tcttcaataa tatctgtaat aggagttgtt gagataatgc gtactgctga tatagtaaaa
    11521 aatgcagcat ttagagcatt agagccatta atagtagcag cagcaattta ctttgttata
    11581 acatttactc tatcaagatt agttggatta ttagaaaaga aactatctgt gtctaactag
    11641 atgagagcat gtttagcatt tagggaaaac atagaatagt aaaaaggaaa gtgataatat
    11701 gattacaata aaaaatttaa gtaaatcttt tggagattta aatgtattaa aaaatataga
    11761 cttagaaatc gctaaaggcg aaataatggt tatagtaggt ccaagtggtt ctggtaaaag
    11821 tacacttctt agatgtatga atttactaga aatacctaca ggtggagaaa taatatttga
    11881 aggaaaaaat ttggtcgata aaaaaactaa tattgatgag gttagacaaa atataggtat
    11941 ggtgtttcag aactttaatt tattcccaca taagactatt ttagataata taacattagc
    12001 accaattaaa ttaaaaaaga tgactaagga agaagcagaa aaaaaagcag aaattttgtt
    12061 gagtagagtt ggtcttttag ataaaaaaga ttcatatcca tcacaattat ctggaggaca
    12121 aaagcaaaga atagcaatag ctagagcact tgctatggaa ccagatatga tgttgtttga
    12181 tgagccaaca tctgctcttg acccagaaat ggtaaacgaa gttttggatg ttataaaaga
    12241 acttgctaaa gaaggtatga caatggcaat tgtaactcat gagatgggat ttgctaagga
    12301 agttgcagat agagttattt ttatagatgg aggaagtata cttgaagata atactccaga
    12361 agaagttttc ggaaatccaa aacatgaaag aacaaaagca tttttagcaa aagtgctata
    12421 atatataaaa aggatgttat tgatattaga aattcaatac atcctttttt agtgatggtt
    12481 aatattaatt ttaaaattaa agttatatta atattaactt tatttttacg attttttgca
    12541 gaaaaaaaag agctcggggt aagctctttt tttccaccga attaatttcc gttactaatc
    12601 cgatttttaa taaatataaa atatatcctt atgaaagggt attgggaata gatatattaa
    12661 ctaactaggg ttaccagggg gataacctta atttattata acaaaatgcg acacaaaaat
    12721 caatattttt tttgaaataa attataaaag ttatttttat aacaagtaag cttataaatt
    12781 taacttttat agtaaaaata agtatatgta taatataata tatttatttt atataatata
    12841 ttattttatc atatattgta ttttataata tatttttgta gggtatagta taagtatatt
    12901 atattttact ataaaaattt gtaataaagt gaaaaaacta gcttttcatg aaataatatt
    12961 ttataattta agattgtctt tttaataaag attaacaaaa aaaatactat aattagtggt
    13021 ataatagtta gagttgaaga ttttaaataa aggtaggtgt aattaagttg gaaatatact
    13081 tagacaatag cgcaactaca aaaccttatc aagaagttat agataaaatg gtttatgcac
    13141 ttaatacgga atatggaaat ccttcttctg tccatagaaa aggtgtagaa gttgaaaaag
    13201 ctataaaaga agtaagacaa gatatagcta aatctctagg agcgaaagaa aaggagattt
    13261 attttacatc aggtggtaca gagtgtaata atactataat aagaggtatt actagtttaa
    13321 ataaaaaaag aaaaaatcac ataatatcta ctaacataga acatccttct gttttaaata
    13381 cattaaaaga tttagaagaa gatggttttg aagtaactta tttagaggtc ggaaaagatg
    13441 ggaaaataaa tatagaggat ttaaaaaatg cgataaagtc aactacatgc ttagtaagca
    13501 tgatgcatgt taacaatgaa ataggaacta tacagccaat aggtgaagtt ggaaagtatt
    13561 tgaagggttt aaaagaaaaa atttatttcc atgttgatgc tattcagtca tatggaaaaa
    13621 ttaactttag accatctaaa tacaatatag attttatgag tgtaagtgca cataaatttc
    13681 atggtccaaa gggaattgga tttatgtata taaaagaaaa taatcgcctt aagcctatgt
    13741 taacaggtgg tggtcaggaa attggaatta ggtcgggaac agaaaatgta cctggaatat
    13801 atggtcttgg agaagctgtt agaatactaa acaaagattt agatgctgtt atttcgaaag
    13861 tagataactt aaaaaatata ttaaaaaatg aaataattga taatatagaa gatataaaaa
    13921 tcaattctcc agaagacgga gtatgtcata tattaaatgt atcatttaga gggacaaaag
    13981 gagaagtttt acttcattac ttagagcaaa aaggtattta tgtatcaact ggctctgcat
    14041 gttcttctaa aaagaaaggt agttacgtat taaatgctat aggtcttaca aatgaagaaa
    14101 taaatggaac aataagattt agtttatctg atatgaatac agaagaagaa atgttagaag
    14161 ctgtgaaagt tttgaaagaa tcaatttgtg acttaaggtc aataatgaaa agaaaataaa
    14221 gaatactgta taaatagtga taataaatta gtttaagagg tgaaatggtg tataatatat
    14281 taatagtaaa gtatggagaa attggagtaa aaggaaagaa tagatatata tttgagaata
    14341 ggcttataag aaatataaga aatatgttaa aaccaattgg aagatttaat gtatataaag
    14401 agtatggaag aatatatgtt gatttagaag actatgatta tgaagaagta attgaagaag
    14461 ttagaaaagt atttgggata gtgggtgtgt gccctggagt aagagctaaa aaagattatg
    14521 atacattaaa agaaatagct ttaaaaatgt tagaagaaaa aatagaggct ggatataaaa
    14581 cttttaaggt agagtcaaga agaggagata agtctttcgg attgacttct caagaaatga
    14641 gtatggatat aggtggatac ttattatcaa aggtaggaga taggattaat gttgatgtaa
    14701 gaaatccaga agttaaaata aagtgcgaat acagagaatt tcatactatg gtctatagtg
    14761 atactatacc tggatatggt ggattgccac ttggaacgaa tggtagagct atgtctcttt
    14821 tatcaggagg tatagatagc ccagtagcta catggatggt tgcaaaaaga ggtatggaag
    14881 tagaggcagt tcactttcat agttatccat ttacaagtga aaggtcacag gaaaaagtta
    14941 aggatttagc taaaatactg gcaaaatact gtggtagagt aaggcttcat aaagttaata
    15001 tcttagaaat acaaaaagca attggcgaga attgtaatga agaagaatct actattcttt
    15061 caagaagatt tatgatgagg atagcgcaga gactttctga aaaaagacat tgtgatgctt
    15121 taattacagg tgaaagtatt gggcaggttg catctcaaac tatacaagga cttacttgta
    15181 ctaatgccgt tgtagactta ccagtgttta gaccacttat agcaatggat aaatcagata
    15241 tagttgatat agccaaaaaa atagggactt ttgaaacatc tatagtgcct gaagaagatt
    15301 gttgtagtgt attttctcct agaaagcccg tcactaaacc tagattagag aaaatagaaa
    15361 aatctgaaac agcacttgac attgaaaaat tagtacagga tgctattgac aaaatagagg
    15421 tagaggatat tgagttttag catacaagtt atttaattac taaattgaat attgagtata
    15481 ttagtagttc tgtaagtata ttagtagttc tgttgaaagg atttatgtag acaatttatt
    15541 aatatgtagt atttaaaaat aagtactata attttattta atgggaatga ttattatgaa
    15601 taaaaagggt tttacactaa ttgaattgtt agtagttata tctataatag gcattttagt
    15661 tatagtagct gttccagcat tgtttagaaa tatagaaaaa agtaaagcag ttacatgtct
    15721 ttctaataga gaaaatataa agactcaaat tgttattgca atggctgagg aatcaagtaa
    15781 aggcaagaat gaagtcatga aagaggtatt agaaaacaaa gatggtaagt actttgaaac
    15841 agaaccaaag tgtaagtcag gtggaatata ttcagcaacg tttgatgatg gttatgatgg
    15901 aataactgga atagaaagca ttgcaaaagt gtatgttact tgtacaaaac atccagatgg
    15961 tgttgaaatg gctagggata tacatcaaag tatgaaagat ttgattgcat catttgcaca
    16021 agacccttct ataataccag gagcctcaaa gggtaatgat gattttagaa aatatttatt
    16081 agacaataaa tataaaaatg ggtggcctac aattccagat gaatttaagg caaaatatgg
    16141 attaagtaag gatacactat atatacaacc atatgcatat aatcctacta aatctgatgc
    16201 tactgtagtt gtatttgcaa ataataagac tgggggtaat tggtatactt ccctagttta
    16261 cgattatgat gaaggtagat ggtataaagg taaaaatggt atttctgttg caggtaggtc
    16321 atgggatgtt gacacagata gtgttaagtc tgtaaaaaca gagattcatt ctaaagaggg
    16381 atggggtcct ttaaattaat atatcatgtt ttattaatct ggtattgatt aaactataga
    16441 aaagagcagt ttctatagat gacgaatacc agattttcat ttatatatat aattgagata
    16501 tattaaaagt gtttatattt ttaagtcttc acatacaagc taaaaataat tataataata
    16561 ttgtaaagaa atttaattat aagattaatt ttctgaagat ttagtatttg tactaagaaa
    16621 tacataaaaa ggggaataga tatgaataat aattttcaag acaaccaaac aataaattta
    16681 atacaaagtc gtagaagtat acgaaaattt acgacagaac aaattagtga tgagcaagta
    16741 aacacattat tacactgtgc atttgcagca ccttcaggat gtaataaaca gccttggcac
    16801 ataactgttg tgcaagacca gaaactttta aaagagatta gtgatgatac tttatctagg
    16861 attcatgagg tgagtaatgt tgagataaat aaaaacttta agttatttta tggtgctcca
    16921 accgttttat ttatttctta tgatgaaagt agttcatggg ctccatatga tataggaata
    16981 cttacaggga atataacaac agctgcacaa gcactagggc ttggaagttg tataatagga
    17041 atggttagag gtttatttac acctgttgaa caaggggata ttgaaggtct tgtgagtgta
    17101 ttggataaag aagatgtaaa agaatcagag tcaataaaga tgaagtttga tacaaataaa
    17161 aaatacagag aactacttga tataccagaa gggtattctg taccatttgg tattgctgta
    17221 ggtattccag atggaaattt accaaatgct agagaggtag tttacaaagt ttccagagtt
    17281 taggataaag tattgcaaga accaatcagt tacttgaaga attaaatcag taatatacaa
    17341 ataaattgat ggaagaggaa gctatttaaa aagtagttta gatttttcgt tgtaaaataa
    17401 ttaaataaag aataaggtga taagtatttt atttattatc ttattctttt ttatttttgt
    17461 tttgtagaat gataaaaata aggattaagt gtaataaaat agaaaacaag tatgttaatt
    17521 ttaagtatat gctttaaaat ttatccaagt taaaaattta agatatcttt tcagtataat
    17581 ggaaaaaatg tcaaaatatt gaaataggga aaaaatagtg atataataag aaaaaataga
    17641 gaaaa
//
LOCUS       DAYAOY010000091        17556 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYAOY010000091 DAYAOY010000000
VERSION     DAYAOY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.091"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     misc_binding    174..405
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            513..1994
                     /gene="thrC"
                     /locus_tag="ACGI6B_003021"
     CDS             513..1994
                     /gene="thrC"
                     /locus_tag="ACGI6B_003021"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFG9752438.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2004..2897
                     /gene="thrB"
                     /locus_tag="ACGI6B_003022"
     CDS             2004..2897
                     /gene="thrB"
                     /locus_tag="ACGI6B_003022"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFG9752439.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3027..4082
                     /locus_tag="ACGI6B_003023"
     CDS             3027..4082
                     /locus_tag="ACGI6B_003023"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFG9752440.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(4237..4749)
                     /locus_tag="ACGI6B_003024"
     CDS             complement(4237..4749)
                     /locus_tag="ACGI6B_003024"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFG9752441.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5269..6204
                     /locus_tag="ACGI6B_003025"
     CDS             5269..6204
                     /locus_tag="ACGI6B_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFG9752442.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6296..8167)
                     /locus_tag="ACGI6B_003026"
     CDS             complement(6296..8167)
                     /locus_tag="ACGI6B_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9752443.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHILS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8357..9283
                     /locus_tag="ACGI6B_003027"
     CDS             8357..9283
                     /locus_tag="ACGI6B_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFG9752444.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9422..9568
                     /locus_tag="ACGI6B_003028"
     CDS             9422..9568
                     /locus_tag="ACGI6B_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752445.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9673..10197)
                     /locus_tag="ACGI6B_003029"
     CDS             complement(9673..10197)
                     /locus_tag="ACGI6B_003029"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9752446.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10250..10474)
                     /locus_tag="ACGI6B_003030"
     CDS             complement(10250..10474)
                     /locus_tag="ACGI6B_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752447.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10671..11795
                     /locus_tag="ACGI6B_003031"
     CDS             10671..11795
                     /locus_tag="ACGI6B_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HFG9752448.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12269..13093)
                     /locus_tag="ACGI6B_003032"
     CDS             complement(12269..13093)
                     /locus_tag="ACGI6B_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HFG9752449.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13441..14496)
                     /gene="ispG"
                     /locus_tag="ACGI6B_003033"
                     /gene_synonym="gcpE"
     CDS             complement(13441..14496)
                     /gene="ispG"
                     /locus_tag="ACGI6B_003033"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFG9752450.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14679..15683)
                     /gene="rseP"
                     /locus_tag="ACGI6B_003034"
     CDS             complement(14679..15683)
                     /gene="rseP"
                     /locus_tag="ACGI6B_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFG9752451.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15696..16850)
                     /locus_tag="ACGI6B_003035"
     CDS             complement(15696..16850)
                     /locus_tag="ACGI6B_003035"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFG9752452.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(17014..17217)
                     /locus_tag="ACGI6B_003036"
     CDS             complement(17014..17217)
                     /locus_tag="ACGI6B_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752453.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         6056 a         2718 c         2082 g         6700 t
ORIGIN      
        1 cactatgaaa atctaggttt ttaaactttt aaaaaggtta aaaaaatatt tttctaatac
       61 tcaatttgat tacaattttt ttacaattta aaaaatattt tttccaaatt tttatattaa
      121 tacttgactt tgtctgtttt ataaactaga ataattaata atattaagaa tattgaaagg
      181 cattgattag gaagagtaaa tctgaatcga ttaccagaga aggaaactac tagctgaaat
      241 gtttccctaa ttagaaggat tgaaaactac ctaggagctt ctatccgaag ttatcttaat
      301 gattttagta ggaatagacg tttttattac ttgcgttaaa gtatcaagtg gattttttaa
      361 aatcaatttg ggtggaaccg cgggaaacag attctcgtcc cttttttagg gatgagggtc
      421 tttttttatt acctaaattc agtttttaaa atattctgta tctaatttaa ttagctaatt
      481 aaaaatttaa aatatatgga ggattataaa aaatgtatta ttcaagtacg agaggcaccg
      541 aagaaaaagt aactgcatca caagcaataa taaaaggaat atcaaatgat ggtggtcttt
      601 atgttccaag tgaatttccc aatgtaaaaa atgaacttat aaatttagta aatttaacgt
      661 attctcagat agcatttttt gtacttagta aatttttatg tgattttact gaagatgaaa
      721 ttaaaaattg tatagaaaat gcatatgatg aaaagtttga ttgtagtagt atagctccat
      781 taaataaagt aaatgatact tatttcttag aactatatca cggtccaact ttagcattta
      841 aagatatggc acttactata atgcctcatc ttcttaaaac atctataaag aaagataatt
      901 tagagaaaga tgttgtgatt cttactgcta catctggaga tacaggtaaa gctgcacttg
      961 agggatttaa agatatagat aaaataaaga taattgtatt cttcccagaa gatggtgtaa
     1021 gcccagtaca aaaacttcaa atgaaaactc aaactggaaa aaatacatat gtagttggta
     1081 taaaaggaaa ttttgatgat gcacaatcag gtgtaaagaa gattttttct gataaagaat
     1141 ttaatcaaaa actattggat aataattata tattatcttc tgcaaattca attaatatag
     1201 gaagacttct tccacaagtt gcatactatt tttattcata tatgaaatta gttagcaata
     1261 gtgatataaa tttaaatgat aaaataaatt ttgttgtccc aactggtaat tttggtaata
     1321 ttctagctgg atattatgct aaacaaatgg gtcttccaat aaacaaatta atatgtgcat
     1381 caaatgataa taatgttctt tatgatttct ttaaaactgg tgtatatgat aaaaatagaa
     1441 cattgaaatt aactacttct ccttctatgg atatattaat ttcaagtaat ttagaacgat
     1501 tactgtttga aatatcaaat agaaatactg atattgtaaa taagctttta tcagacctta
     1561 gtgataaagg agtctacaaa ataagtgatg atatggaaaa aaatttagcc tcattctatg
     1621 gagaatattc aaatgaagaa gaagttaaaa gcacgataaa caatgtgttt aaaaattata
     1681 attatttgat agatactcat actgctgttg catacaattg ttatgaaaaa tataaaaaag
     1741 aaacttctga taacacaaaa actgttattg taagtactgc aagtccattc aagttttctg
     1801 aagatgttct aaagtctata gattgtgatt ttaaagacct tgatgacttt gctataattg
     1861 atagattatc aagtatttct aaaatagata ttccaaaacc tattaaaaat cttaaaaatg
     1921 cagaaatact acataaagat atttatgaaa aagatgaatt aaaactagct ataaaaaaat
     1981 tcttaaaggt ataggtgatt attatgttag agattatagt tcctgcaaca agtgctaata
     2041 taggtccagg atttgattgt cttggaattg cgttaaatat atacaataaa ttttatgttg
     2101 aagaaattga aagtggtctt gaaatagaag gttgtgaaga tgcttacaaa aacgaaaaca
     2161 acttagttta tacatctatg aagtactttt ttgatagagt taaaccagaa aaaatacctg
     2221 ctggtatcaa aataaaaatc caaagtgaag tacctatatg tagaggtctt ggcagtagtg
     2281 cctcttgtat agtagctgga gttatagctg caaatgctct atcaggtgct aatttagata
     2341 aaaatcaatt actaaatata gcttcagaaa tagaaggtca cccagataat gttgctcctg
     2401 ctattttagg aaatatgatt gtatctgtta ctgataatga gaatatacat tatgatatta
     2461 taaaaattcc tgaagaactt aaattttgtg caatgatacc taattttaag ttatcaacag
     2521 aaaaagctag aggtgtttta ccaaaggaaa taccatattc tgatggggta ttcaatgtta
     2581 gtagagttgc tttactaata tctgccttac taaacaaaaa ctttgaccta cttaaagtag
     2641 cttgtcaaga caaattgcat caagattata gaggaactct aattgagaac tacaatgata
     2701 ttgttgaaaa atctgaacaa ttaaatagta ttggagtatt tttaagtgga gctggaccta
     2761 ctataatgtc tttaataaaa gaaaatgatg atagttttgt ggataatatg aaaaattacc
     2821 tacaaaaact aaaatctgat tgggaaataa aagagttatg ttgcgattct aatggagctg
     2881 ttttaaatat aatataatta tcgtcttcta tacaaaatat aactactctt ttattataat
     2941 ttgtagtata ataattagga ctaggtaata atattaagta ttataatcta gttctttaaa
     3001 catgtttaca ttgaggaggg attatcataa aagagttaat aattagagat ttagttgcaa
     3061 aagttcctat aattcaaggt ggaatgggcg ttggtatatc taggtcatca ctagctggtg
     3121 cagttgctaa gcttggtggt gttggtgtaa tttctggagt tcaaattgga tatgatgagg
     3181 aagattttga aactaatact ataaatgcaa atttaagagc tataaaaaaa catatttcta
     3241 aggccaaaga aatctcaaat ggtggtatta ttggtattaa ttttatggta gctatgaaag
     3301 aatatgaaac atacgtaaag gaagctgtta aagctggtgt agaccttata atatctggag
     3361 ctggattacc taataagcta ccatcattag taaagggaag taatgtaaaa atagcaccca
     3421 tagtttcaac tgcaaaagct gctaatgtaa ttttaaaaat gtgggataga aaagagaaaa
     3481 ctactgctga cttaatagtt gttgaaggtc caaaagctgg tggtcacctt ggatactcta
     3541 acgaggaact tgataacata gattctatag actatgataa ggaatttgtt gaaattttaa
     3601 aggttgcaaa tacttatgga gaaaaatttg gtagaaacat accagtagtt gctgctggtg
     3661 gtataacttc tagtagtgat gttaaaaaat atatagatat gggtgcaagt ggtgtacaag
     3721 ttggtacaag atttgttgct acttacgaat gtgatgccca tgagaacttt aaaatggcat
     3781 acataaatgc tagtgaagaa gatgttcata tagttaaaag tcctgtaggt cttcctggac
     3841 gcgccataag aaacaaattt atagaagaag ttaaaataaa tcgtccagaa ataaagaagt
     3901 gctataattg tttgattcct tgtaatccaa aagaaactcc ttactgtatt tctcaagctc
     3961 taatcaatgc agttaaagga gatgttgaaa attcactttt attttgtgga aatgatgcct
     4021 ataaaattga taaattaagt actgttgaag atgttatcaa tgaattgatt tctgaattat
     4081 aatttaaaga aaactaaatt aaaattttat caagctaagg agtttaaata cgttatttat
     4141 taatctttat aaacatattt atgtccttag cttttttgtg tcaatttcct tttatggaaa
     4201 ttttaattta attagagata gtattaacct ttataactaa tcttctttta caagtacatt
     4261 ggttatttca gctatatata aagtatgata atctttttgt ggataccatc ttccatctat
     4321 accttcagca ataaaacatt gaggttcaag tttctgacta tacatttttt tgcatataat
     4381 agccatttta gattctttaa aataaggtgt atcatttaag tgttctatat taaatccaat
     4441 ctgtgatatc ttatcctctt ctctaccaga tacctttcca caatatgaaa gctctttacg
     4501 aaaatcttca tcaaaaaatg taagagaaaa tttatctgta ttatctataa attccttagt
     4561 ataacgctgt ggtcttatta ctacaaaagc aacatctttt ccccacataa ctccaagccc
     4621 tccccagcta gcagtcattg tattcacttt accttcattt tctgctgtta taagtgtcca
     4681 atctttacct atcaatccaa atggattatt tttaatctct tctgccaaaa tctctttaaa
     4741 atcactcata tctctaataa cctctcattc tcttatattt atattccttt tattttcgtg
     4801 ataaattata aatgaagtct agttttgaaa ataaaatact tttttaaata tattattgca
     4861 aataagtaaa tatatatata ctctcttatt ttttaatata tttaataatt tcactcagaa
     4921 tatatatatt ctttactcaa attaaatata acttatatac tttatgtgta aattatggta
     4981 aaaatattat ttaatcaaat caaaacttga tttatatacc tggaaaaggt attatattaa
     5041 agagtgctta aaatacgcat ttcataaata gctatatata aaataataaa taaggagagt
     5101 gatgggcaat ggatactagt ccgttgcgga gagaattttt tacattttca tcacttaata
     5161 atatctaaaa tcaaaactaa tacatatatt cagttatata aattagttaa ttaatagata
     5221 ctattaaatg atgagattta tataaaactg aataaaggat gtgttgccat gataagatat
     5281 tacaaaacta tagattcaaa attagagaaa cttagttttt ttgaagatgg ctgttggata
     5341 aatcttgttg aacctaatca tagtgaaatc aacgaaattt ctaatttatt aaatatagat
     5401 gtagaaagca tagaatctgc actagatgaa gaagaacgct caagaataga tgttgaagac
     5461 aatcatactc ttatacttat agatatacca gtagacgaaa gtgactctaa ttcttctcat
     5521 tatactacaa tacccttagg tattatacta actgaagaag ctattgtaac agtttgtgat
     5581 gcacaaacta agattttaaa tgactttata gttggtcata ttaaagattt ttttacgttt
     5641 aaaaagactc gctttttgct tcaaattctt cacaaaaatg cagcttatta cttgcattac
     5701 ttaagaaaaa taaataaaat gacaatcata atagagcgag aaatttacaa atcaatgaaa
     5761 aacaaagaat tggttcaatt attggaatta gaaaaatctt tggtatactt ctctacctca
     5821 ctaaaatcta atgaacttgt tttaaacaaa atggttagaa ctgctggtat aaagaagtat
     5881 ccagatgatg aagacctatt agaagatgtt atagttgaaa atagacaggc tcttgatatg
     5941 gctaaaatat atggagatat attgagtaga attatggatg ctttcagtgc aattataagt
     6001 aataatcaga ataatgttat gcaaatttta acagtagtta cactaatttt ctcaatacct
     6061 acaataatat caggattctt tggtatgaat gtcatcaaca tgccattttc taataaccca
     6121 aacggatttt ggataatact tcttattacc tctataatat gtatagtgat tacatttttc
     6181 atgtcaagaa ataagctatt ataaaatatt aataaaaagg ccattagaat taattgattt
     6241 acatttatcc taatggcttt tatatattta aatctatata atcttataat actttttaac
     6301 tatcatgttc ttcatctata atcatttcag tttcaagccc ttctagcatt gaatctaaac
     6361 tttcttttat atttagtata taaattggta catactcttc ttcatctatt acctcttctt
     6421 gcaacttaaa tggaggaatt aatacttcat caaagtctat atttaaattt ggaaagcctt
     6481 ctactatctc ttcacaatta tcgtactctt catttgctaa agaaatgtac atatctaggc
     6541 tctttttata ataaagacct gcaagcatat actcacactt cttacttcca tatttaatac
     6601 cttctttata atatttctca gcttctgcgt catcatttaa ttttaaataa atttgaccta
     6661 aattatatat tgattcaaca caaccttgtg ctctagcctt ttcataccaa aatttagaat
     6721 ttacataatc tttatagaac atatcataaa ttaatcctac catatgttga gcatacacat
     6781 tattttcatt ggcaggtgta tcaaacatca ttttagactc ttccaattta ccttctctaa
     6841 aataaattct acctagatgt atacgagcat tcatatggtt tttgctactt gccatttcaa
     6901 aatactttct agcatttttt gtatttccta agctctcata tatacacccc aatctataac
     6961 tagacctcgt atctccattt tcagatgctt tcttatacca acttattgct tcttcaatat
     7021 tatttaaatc ttcatagata tttcctaatt tgatttgaca agatacatta tttggaatcc
     7081 tcgaataata cgaaatcgct ttttctatat cttgattctg ataatataaa tcccctagat
     7141 tttcaaggga acttttgcat ccaacagcta tagcatcctc ataatattta atagcatttt
     7201 cataatcttt ttcttcaaaa tatatacctg ccagattatt ttttgcttct gttatatcat
     7261 cttcagcagc cattttataa taagtttttg ccttctctat ctcattttct ctattatata
     7321 aattagcaag tctatactta gaaagtatat gatttttttc tattgctttc tcataataat
     7381 aaattgcatc tcccagctct tcttttctat catatacata tgcaagccta tactcagact
     7441 tcacatcatc tctatcagca ctctttttat aatacttctc tgcatcttct aaattatcat
     7501 ttatttccat aatcataccc aagttaaagc aacctttcat atgacctgac ttgtacaact
     7561 gtttatataa acttactgtt gatttattat ctttcttttt tccatttaat attgctaaat
     7621 tatattttgc atcatcatct aaaagcacag aacctttttt atagtattca tatgcttcat
     7681 catctttctc caaaaaagta tataataccc ctaaattaaa acaagcttta cctaaattct
     7741 tgtctgctgc tgtttttaac catttttcac accttacaaa atctttcatt ttaaaatggc
     7801 atacacctaa attattttgt gcttgtaaat aacctaaatt tgctatcttt tcataccaat
     7861 atatagcata ttcatagcca tctaacttat agtacatatt acctaaatca aaagcagcat
     7921 cttcacttcc acttttatat gccatcgaat accacttttc tgcttcttta aattgttttt
     7981 tactttcata tatatttcct aaagtatatt gagattttac gtaattatcc aatgcagcac
     8041 tcttatacaa ctctattgcg ctttccatct tacccatctt ttcaaggagg gcacccttag
     8101 tatgtacgac tttcgggtca ttagatatat tttttatttt ttttatagaa aattttgtat
     8161 ttttcattat tcgctaccta cccattttat aaaatatatt ctctcaaaca ttattataca
     8221 ttataatcta caattaatgc tataatattg aatttttacc ttttgtcact atattgtaac
     8281 attaaatttt aaattttata atcattagat tttattatgt aattggtata taatgtatat
     8341 atagggggga cattttatga atttcagcaa tatttttatc caagttgcag ttttatttat
     8401 aattatttta gttggatatt ttgtcagaaa attcaactta ttagatgacc actgcacatc
     8461 taagctttca actttgacaa tgacagtttt tttaccatca atgataatta gctccatgca
     8521 aattaacttt gatagtaaaa tgatacaaaa aatactattg ttattattta catctttgat
     8581 tatgtatata gtttcaatta taattgcatt tttattgaaa tacattttaa aatgtaatga
     8641 taaaaaagat ttgggaatat accagtatat agttgttttt tcaaatgttg cttttatggg
     8701 ttatcctgta attgaagcag ttttaggtca tgaagcaata ttctatactg caatattcaa
     8761 tttgcctttt aacttatttt ctttcacttt aggaatttat cttttatcta aaggcagtac
     8821 aagtagaggt ttttcaataa aatcacttat aagccctgct acaatagcta ttgttattgg
     8881 acttttttta tttgtaacag gacttaggtt accccaattt ataaacgagc cacttgagat
     8941 gttgggaagt ataactacac caatatccat gattatcatt ggaagcttac ttgctaattc
     9001 ttcagctctc gattgttttg tcaacaaaaa attatatata gtcactttta taagattatt
     9061 ggtacttcct gtgattgtgt attttattct aaaaggatgg ataaatgata agatgatttt
     9121 agctatacca gtagtaattt cttcaatgcc tgctgctgca aatactgcta taatggctaa
     9181 tcaatatgac tccaatataa cactagcttc acaatgtgtg ttttttacaa cactattttc
     9241 agttatatct atacctttta taagtatttt actcttatct tgatttattt ttttatgtat
     9301 tatataataa catattcaat aaattacaaa tataaacatt ttattgtagt atattgatgg
     9361 aattttttcc tagatatttg tcaataatat ctatatgtat tatagaatag gaggcttaaa
     9421 tatggatatg gatttactct ttaaaatagt atttgctata gttgctatag gtgtttctgt
     9481 aaaaatcatt aaaactgttg ctagtttagc ttttaaaata gcgttgatat tgctaataat
     9541 tttatttgta tatagaatgt ttttgtaaac atttaaaaat aagtttttta acacaataga
     9601 gaagtaaaat agattatctt taatgtaaat tttttattaa agacactcta ttttacttct
     9661 ctattagctt ctttaaatta aatttttatt atatcttccc ataatataaa catcttcaaa
     9721 ttttccatct ctgattacag catatttttt agttccctct atttcaaaac ctagcttttt
     9781 atataaattt aatccctttt cattatccac aattacttct aatcctactc tagttaacat
     9841 aatccaatta tctgctatat ctaaaagttg ttccataagc ttcttaccta ctcctcttcc
     9901 ctgataattc tctgcaacac ttattcctat ttctgcgcta tgtctcaatc gtggagaaga
     9961 atttacatgt aacccagcaa aacctattac atctttttct ccattattat tagtttcagc
    10021 tacaaacata taatcatttt ctcccaaaga agctaacata gcttcagttt tatcgactct
    10081 gtcactcatt aatgccaaag tatttctcat aacactgtat gttcttctaa tttcattaaa
    10141 agcttgtgca tcttctcttc ttataggtct tattgttata tctataggct tactcatatt
    10201 ggattccccc tcaaacataa tctttatttt taattaaaat tttacatctt tattttatac
    10261 tgttcaaatc tatgtatgct tctacctgag gtacatatat catattatct tttatatgtg
    10321 tacttccaaa ttcttttaat tctgtacctt ctttatcctt agttatttta tatctaatgg
    10381 attccatacc ttcaactact tcagcatgaa aaaacatacc ttcatattgt ttaaatctaa
    10441 aattaaaatt gtctttagta agttttatta tcatattttt atctactcct tatataattg
    10501 atatttttct tattattagt tgtatatata ctgcttattc atattaatat attataactt
    10561 atcatttatt tagtataaag ttataaatag atatatttat acttatttta caatctttct
    10621 tcaattgata tataatatgt ttatataaac taatgtatgt gaggtgaaat atgagcaaaa
    10681 aaacaaaata tttttcttta attctctttc ctattgcttt gcttttaaat tttatagcca
    10741 gtaaagttcc aaatattgtt gaaaaatatt actcccaatt catagataaa ataattgtac
    10801 aaatattgag taaagtttct ggaattttcc cattttctct ctatgagatt acaatgtaca
    10861 taatagttat atctaccttc ttatttttat gttgtacttt atctacaatt tttaataaga
    10921 aaaaaaaatt aaacatatat ttaaaaaatt ccattttaaa tatattatcc attgtatcaa
    10981 tagtctactt tctttttgta gtgctttggg gaataaacta taataggatt ccactagaaa
    11041 ccacacttat aaataactat aatttaaaaa acaatacctc tatccaatca aaacaatata
    11101 gtgttaaaga gcttactaaa ttatataaat tccttgtaat taaagcaaat gaaactagaa
    11161 aactcacatt acaagataaa aatggtattg taaaatctaa tacagattat aatggtgtca
    11221 taaatagagc tcaactagga tacgataata tcttggatat attacctggt gttagtgata
    11281 gttattctaa gcctaagtat attatttcat caaatctaat gtgctataca ggaataactg
    11341 gtatatactt tccttttaca ggtgaggcaa atgtaaatat agcaatacct gacttatata
    11401 ttccttgtac agttgaacat gaaatggcac atcaaagggg atttgctagt gaagatgaag
    11461 ctaattttat agcgtatctt acttctataa aacaccccaa tattgatttt aactattctg
    11521 gatatatttt agctttaaat tatactgctt ctgctttaag taaggttgat tataatgcat
    11581 atgttgatat ttctgctggc atttcagatt ctgtccgtag agatttaaaa aatgaaagtg
    11641 aattttggca aaaatatgaa ggtaaaatta atgaaatttc taatgagttt aacaattctt
    11701 acttaaaagc taatggcgtt actgaaggta ctcagagtta tggtaaaatg gttgatttat
    11761 tgctaactta ttatgaatta tatccatata actaaacact acaattcaaa attttattca
    11821 tatacagctg atattaaatc agctgttttc tatttaaata aattatttaa cttcaagcaa
    11881 tcttatataa tttgatagta cttatactat ctgtataaca tttcaattct aactatttta
    11941 gttaaaacat ttctattatc ttttttaata aatgttttaa ctaaaatttt taaaacataa
    12001 aacttccaca ttatttcact atctatataa aaatctaaaa attcacatat aaaattacat
    12061 aactaaaaaa tcataaattt atagaaaaca tatattcaaa ataaaaaagc atgtatcaaa
    12121 acttgacaca tgcttataat cttatgtata tatatcattt tgtcacaata aaaatttact
    12181 tgtaacactg atatttttaa acaaaatagt ccttatttaa ttaaattaac attaattaaa
    12241 ttcgtactca ttttgctatc attaaacatt acttatagta attttctaat acactatttg
    12301 aaacttcctt cccttgaaaa cttttcaacc aattctcata tgctgagttt ttccatacac
    12361 taccatcatt aaatgttact tgttttacac aagatataat gtactttaca ccatgctctc
    12421 cagttgattg tttccattta tcaaacaata gaaaggcatc attttgattc ttacctggtg
    12481 ctattgcatt ttgctttgca tattctttct ccatttgttt ccattctttc aaccaatttt
    12541 ctacccatgc cttgccttgt tgtttttcaa acatacttat tatatcctgt ggtggtgatt
    12601 gttgcatttt tctataagta tgtgaatatg attttggaga cacaggagat atgcctgtaa
    12661 caataccata gtctacacca gctgggctaa atccaacact tcctacttcg ccatctgctg
    12721 ccacattttg agcatcccaa tatagctcaa gtggttttcc atttttatca tatgctaagc
    12781 atgtaatttc ataatccata attgcctttt cactgctatt tgttaagctt ataacattgc
    12841 taggccaatg atatggagta tcatcatact gtattttgct attatttata aataatggaa
    12901 tattctcatt ataaacaaga cctttactac tttgaatatc tgataccttc tttgtgacag
    12961 tacttaccgt atttgctaag tatacatttg ttttttctga attggtcgta ctaagagaaa
    13021 atgttgttgt cgttccagta atcagcaaag caacaactaa aataggtaag ctagatattt
    13081 ttttatatct cacaattctt cacctctttt tctattaact tataataaaa caatttacca
    13141 aaacattaaa atctatgtta acataattta ccaactgttc tagtaactat atcctatcat
    13201 agttactatc aagtttcaat actattgatg gaaattttca ctatattaaa tcttttttta
    13261 attttataaa tttacaatat gatttaactc atacatatat agtcaaatta gaatataagt
    13321 ttcttacatt taatgaagtt tataaaataa aaaatgacta gataattaaa tactgtaaat
    13381 aaaaacttat tacaaaagta tttaaaaatc tagtcattta atatatatat gaattgtata
    13441 ctataatttt tcaatctctt ctagcaactc atcaactaat ttgttaccat caatcattct
    13501 aactatttca ccctttttaa ataaaagacc ttcaccttta cccccagcaa tacctatatc
    13561 tgcttctctg gcttcaccag gtccatttac agcacagccc ataattgcaa cagtgatatt
    13621 tttttccata ctaccaatct tttcttcaac ctcatttaca acactaatta agtctatatt
    13681 acatctacca catgtagggc atgatataac tttaatctta tcatttaaaa gtcccaaact
    13741 tcttaaaatc tgtttaccaa caataacctc ttcaacaggg tcgcctgtta aagatattct
    13801 aacagtatct ccaattcctt taagaagaag tgctcctacc cctatagaag actttattgt
    13861 tcctttgtga acacttccag attctgtaat tccaatatga agaggataat caactttttt
    13921 tgatattaat tcatatgcat caacagtttt ataaatatcg gatgatttta gagatataac
    13981 tatattatga aaatctaagt cttcaagaat cttaatatgt cccattgcac tttcaactaa
    14041 tgcttcagca gttgcagagc catacttttt taaaagttct ttctctaaag aacctccatt
    14101 aactccaatt ctaattttta aatttctttc cttgcatttc tcaactacca tcttaactct
    14161 ctctatactt ccaatattac caggatttat tcttattcca tccacacctt gttctatagc
    14221 ttcaagggct agcttataat cgaaatgtat atctgcaata attggtatat ttacactaga
    14281 ctttatcttt gctatatttt tagcagcttt aatatcagga acagcaactc taactatatc
    14341 acaaccagct tcctctaatc tctttatttg agctattgta gcatctgcat ctcttgtatc
    14401 agtatttgtc atagattgta tacttatagg attttctcct cctattttta cagctcctac
    14461 acttacttct ctcgtctttc ttctctcgta actcatattc cacctctata tattatttta
    14521 tattgaatct ctttaaatat taaaatatta taacctacat gtatattata aattaaaaag
    14581 ctatctagtt aaataagaat aaatttcata tataaaaatt aatttattct tatttaaaac
    14641 tacatagcct attttcttaa aatctctaaa aaactaaatc attgaaataa tcttagtata
    14701 tctttatatg tgataaaaag cataaataac attaaagctc caaaaccaac aacatttatc
    14761 atgccttctt tatttgggtc gagtttttta ccacctctaa cagcttctag taacaacata
    14821 agtattctcc atccatcaag tgctggtatt ggtaatagat ttactatacc taaatttaag
    14881 cttatgacag ctgctaaata cactacattt atgatgcctg tacgagcagc atctgatacc
    14941 ataccaataa cacctactgg tcctgctaca gcattgccaa tccctcctgg tacacgtcct
    15001 gtaatcattt gacctagaaa tgttagcatc tgttttgtca tatctacagt agttttacat
    15061 gcatttacta ttgaccctaa aaagtctttt tctctttgtg gttgaattcc tatttcatac
    15121 tttccattgt tatttttagg tgttacattt acaactttat cacttccatc tctagttata
    15181 ctaagtttta attctccacc agaagaagtg tttattatat ttgcaacttc atcccaagtc
    15241 ttaacagaat taccattaat tttatttatc ttatctccta cttgaagccc tacagcctgt
    15301 gcaggagtat ctggcattat ctttcctaat ttagtcgttg gagaacctat atacaaaaat
    15361 actggaacta aaagtattgc tgctaatatt atattgaata ttggtcctgc tacaatagta
    15421 ctaaatcttt gtaatataga cttgtttcca aaagaatttg gacttatttg ttcttcatct
    15481 tcaccttcca tgctgacata tccaccaatt ggaagtgctc ttatagaata ctctgtatct
    15541 ttttttacac tatatatctt aggccccata cctatagaaa actcatttac tttgatacca
    15601 cttctttttg caaatataaa atgtcctaac tcatgtatta atacaattat actaaatagt
    15661 attaatgcgg ctattatagt cataaattca ccttcctatt tttttatcag atttgcaaca
    15721 taagctctac tccatctatc tgtttctaag atttcttcta atgttggttc acttatgcta
    15781 ctatgaactt ctaaagtctt ctctatgtaa tatggtatgt catagaaacc aattttatcc
    15841 tctaaaaatt cacttactaa aacttcattt gctgaattta aaacagaaga ataagttcct
    15901 cccattttta aacactcata tgccaatttt aaacatggaa aagtttctaa atctggttct
    15961 tcaaaagtta aagtactaaa ttttgaaaaa ttcattcttt caaagctact ttccattcta
    16021 tctggatatg taagagcata ctgtattggt agtctcatat ctggacatcc taattgagca
    16081 attattgagc tatctgtata ctgcaccatt gaatgaatta tgctttgtgg gtgaactact
    16141 acatctatat tttcttgttc tactccaaat aaccatctag cttctatcac ttcaagtcct
    16201 ttattcataa gcgttgaaga atcaatactt atttttcttc ccatactcca atttggatgc
    16261 ttcaaagctt catttttagt tatatttgca agttctccct ttttctttcc tctaaatgga
    16321 ccaccagatg ctgtaagtat tatcttttct atattttttt tattttctcc atttagacat
    16381 tgaaatatag cactatgttc actatcaaca ggaagaatat ttacattgta tttttctgct
    16441 tctttcatta caagttcacc agcagttacc aacgtttctt tattcgctaa tgctatgtct
    16501 attcccttct ttatagcaca aagagttggg actaatccta tcatacctac tacagcagtt
    16561 aaaaggacat ccacttcatc aagtgatgat attattttta atccttccat tcctgctaaa
    16621 acttctattt ctatattttt aggcaacatt tcttttaatt ttaatgcttt attttcctca
    16681 aaaactgtta catatttagg tttaaactct actatctgtt ctagcaataa ttcaatatta
    16741 ctgttagctg atattgcaac tatctcaaac ttatctctat gttctctaac cacatccaaa
    16801 gtttgtttac ctatagagcc tgtagaacct aaaattgata tctttttcat ataataatcc
    16861 tttctaataa taaatatacc ctgtcaaaac attataacac tactaaaaca attattttta
    16921 ctattcatag aaatttcata tattattcca cagattaaaa ttaatctatt attttttgta
    16981 ttttgtggaa tattataaaa tattctagta ttattaaatg ccctttttta tttccacaac
    17041 atcttctatt ttacagtcta gtgctaaaca tatcttctcc attgtatcca tagaaacaga
    17101 tttatcattt cctagtctgc ctaaaacaga aatacttata cctgatatat ttacaaattc
    17161 cattttcttt atttttctgt caattaacaa tttccataat ggagcgtatg aaatcatgtt
    17221 atcacctcgc aaaatatatt ctatcacatt ttatctattt tggaaaattt tcatctattt
    17281 agataaataa tcacctgctt aagtgtcaaa taattttatc cttctaatat gaaaatttaa
    17341 tactatacta aatgattggt atataacaca tatgatgcat tattattaac atataaaaat
    17401 ttttattggt gctaaattaa agtaatatac tattattata tttgcagata tataagaaag
    17461 attttattat atatttaaag tacttgatta taataagaga caagggctat gatggatact
    17521 atagaaagtt agaactatac tattttagaa tgaaca
//
LOCUS       DAYAOY010000092        17127 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYAOY010000092 DAYAOY010000000
VERSION     DAYAOY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.092"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(221..745)
                     /locus_tag="ACGI6B_003037"
     CDS             complement(221..745)
                     /locus_tag="ACGI6B_003037"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9752454.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(810..1562)
                     /locus_tag="ACGI6B_003038"
     CDS             complement(810..1562)
                     /locus_tag="ACGI6B_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9752455.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1593..2891)
                     /locus_tag="ACGI6B_003039"
     CDS             complement(1593..2891)
                     /locus_tag="ACGI6B_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFG9752456.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2908..5172)
                     /locus_tag="ACGI6B_003040"
     CDS             complement(2908..5172)
                     /locus_tag="ACGI6B_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFG9752457.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5427..6245)
                     /locus_tag="ACGI6B_003041"
     CDS             complement(5427..6245)
                     /locus_tag="ACGI6B_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9752458.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6238..6936)
                     /gene="cbiQ"
                     /locus_tag="ACGI6B_003042"
     CDS             complement(6238..6936)
                     /gene="cbiQ"
                     /locus_tag="ACGI6B_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFG9752459.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6939..7223)
                     /locus_tag="ACGI6B_003043"
     CDS             complement(6939..7223)
                     /locus_tag="ACGI6B_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9752460.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7213..7965)
                     /locus_tag="ACGI6B_003044"
     CDS             complement(7213..7965)
                     /locus_tag="ACGI6B_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFG9752461.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8147..8339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8471..10243)
                     /locus_tag="ACGI6B_003045"
     CDS             complement(8471..10243)
                     /locus_tag="ACGI6B_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFG9752462.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10257..11186)
                     /locus_tag="ACGI6B_003046"
     CDS             complement(10257..11186)
                     /locus_tag="ACGI6B_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9752463.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11407..11592
                     /locus_tag="ACGI6B_003047"
     CDS             11407..11592
                     /locus_tag="ACGI6B_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9752464.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11585..12286
                     /locus_tag="ACGI6B_003048"
     CDS             11585..12286
                     /locus_tag="ACGI6B_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752465.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12288..13211
                     /locus_tag="ACGI6B_003049"
     CDS             12288..13211
                     /locus_tag="ACGI6B_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9752466.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13368..14291
                     /locus_tag="ACGI6B_003050"
     CDS             13368..14291
                     /locus_tag="ACGI6B_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752467.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14284..15021
                     /locus_tag="ACGI6B_003051"
     CDS             14284..15021
                     /locus_tag="ACGI6B_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752468.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            15032..15805
                     /locus_tag="ACGI6B_003052"
     CDS             15032..15805
                     /locus_tag="ACGI6B_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752469.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16346..16954)
                     /locus_tag="ACGI6B_003053"
     CDS             complement(16346..16954)
                     /locus_tag="ACGI6B_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFG9752470.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5535 a         2959 c         2156 g         6477 t
ORIGIN      
        1 tatttaaaaa taaatatata aacaatcata ataaaatttt tttaataaaa ctttattttt
       61 tagttaaaat catacaatat atagatttcc acaatacaaa taaattaaaa agtgaaatgt
      121 atttgtctaa aaacataata acatttcact ttatcctcat ttttaagtaa tatatatttt
      181 taatttttat ttataactaa atctagtttt tatataaatt ttagtttttt tctacatctg
      241 agtaatctat tccaaaagtt tctacagtaa cagatttcat aacttgtgga gtttgtggct
      301 tatcagctga atctgttcta actgatgcta tttcatcaac tgtatccata ccttctatca
      361 ctctaccaaa acctgcatac tgaccatcta agtgtggtga gtttttatgc attatgaaaa
      421 attgagaacc agctgaatta ggtgccatag ttctagccat tgatatgact cctctttcat
      481 gttttaaatt gttagtaaat ccattaccac taaattcacc ttttattgag tgacctggac
      541 cacccattcc tgtaccattt gggcatcctc cttgtatcat aaatcctggt attactctat
      601 gaaatattat tccattgtaa tatccttcat ttactaatga tacaaagttt ttaactgtat
      661 ttggagctat atttggatat aactcaattt tgatttcttt tccattttcc atttctatag
      721 ttactatagg atttttattt tccataattt attaccttcc ttttcattag ttaaattaca
      781 taattacatt tattatatta tactctaaat taaagtctat caagtatatt ttttcctatt
      841 tcatatcctt cttgataaat agttttacaa aactcatcat caaagttaag cgtgtcttta
      901 ggagcaaatt gagtattagg tctaactact attattttat cttcatcata tactttttta
      961 atatcttctg gcaactcata attatatttt gtggatatta aaataatttt atccacatta
     1021 tccccaatta atggttcaat taatgcactc ctaattaaac ctccatctaa tttgaaacca
     1081 tcataaattc cttcttgcac atctttcaca aattgttctt taaatccaag agttgcgttt
     1141 ggatttgatg gtaacaaccc actgtatgaa atgctgataa tggcttcatt acgaggcatt
     1201 tttgatacgt caacaatctt ttctgatact actttattat caacttcaac ataattaaca
     1261 taaaagttca tttcttctat attactatca tctagttccc ttaggttgtc tatagcagga
     1321 gaattatcca cagtattatt tactattttt acatttccat tctggatatc ttttatattt
     1381 gtccacattt tctccaagtt atcaacattc cctgtgtaca aataatatcc atttattgct
     1441 cctattgatg tccctgaaat cgaataaaat ttatttattc ctccatcata caaggcttta
     1501 actactcctg cttgataagc cccttttgct cctccacctt ctaaacataa tcctaatttc
     1561 atagtctcat ctccaaattg tttatcttat atttatattt caaaaaactt actaaatgtt
     1621 ttatatatac tatagtggtc ctcaacattt cctaattctc ttatagaatg cattgctaaa
     1681 attggacttc ccacatccac agatggtata tctatatgag tagaagatat tggacctata
     1741 gtacttcctc ctctttcatc tgacctatta acaaattctt gatactcaac attagcttct
     1801 ctacatatat ttttataaat agctatagaa tatccatcac tagtatatgc ctgactagca
     1861 tttatcttta taaccggacc ttttcccatt attggtttat tagttgggtc atgcttttct
     1921 ggaatgtttg gatgaatagc atgagctaaa tctgatgata tcataaaaga tgaataaata
     1981 gactcaaaga atccctctct atccttaccc attgatatgc aaattctctc caatatattt
     2041 aacaacatgt ttgaatctgc accttgttta gtagagctac caacttcttc attgtcaaat
     2101 acagccatta catttacacc atgttgacct ttagtattaa caagagcatt caaactagca
     2161 tgtaccattg caagattatc ttgtctccca atagatataa attcatcatt agcccctatt
     2221 aaacttcctt tttcaaattc atataaaaat aaatcaaaat ctataatatc gtccatattt
     2281 acatctaagt tagatgctat ttgctttata agaaaattat ctttttctaa tgtatcattt
     2341 aacatcccaa ctaaaggtaa catatctttt tgcttattat atgagtgacc atcatttata
     2401 cttctattta aatgtattgc tagatttggt attatacaaa ttggcttttt aaagtctata
     2461 agcctttcat ttggttttaa tatactatca ccttttaaaa ctactctacc agctatccca
     2521 agaggtctat ccaaccatgt acttaatatt gctcctccat agcattctgt atttaacttc
     2581 aaataagatt tttcagcttc tatttctgca tttggtttaa tactaaatgt aggagaatct
     2641 gaatgagagc ctattattct aaatccatct ctttctatac cttctgaatt tactacaaaa
     2701 gctactatag ctgataaatt cttagtaaca taatactttc caccaactct tagattccat
     2761 ctttgattca aaactaattc ttcaaatcca tttttatcta agatttcctt agtactctcg
     2821 actgcatgaa aagacgacgg actatcatat atgaagtcta ttaagttttt agcaaagttt
     2881 atactatcca ttcctcattt acctccatta taaaatattt agtgttgtat attctttatt
     2941 tattttttta attccctgtt ggtcaaaagc tatagtaaca tctgtaccaa ttatagaaac
     3001 cactgtccca accccaaatt taggatgatg taccttagaa ccaagtttta tatcatctgg
     3061 attagtttct ttattagaat cttttattgt agcattaact ttatctgcaa ctttagattt
     3121 gcttatagta ctcatatact ttttagtata tttatctaac atattataac ttgctttaga
     3181 atagcttagt tcttttttta ctttgtacaa tctttctata cattcttctg gcagttcttc
     3241 cataaatctt gaagctattg caacatttgt tttaccatat aatgttctct tctctgtcaa
     3301 tgacatatat agctcttctt tagctctagt tataccaaca taacaaagtc ttctctcttc
     3361 ttctatttgt gaatcactca tagattttat agctcttgat ataggaaata acccttcttc
     3421 catacctgtt aaaaacacaa cagggaactc taaacctttg gatgtatgaa ttgtcataag
     3481 cgaaacccta tcattgtctt cttcatcatt tgattcagat gtaagtgcta tacttgttag
     3541 gaatgtctct aagtctttat tttcataagt atcatcatta ctactttcaa actccaaagc
     3601 tatagaaata aattctctca aattatcaat tctgtcttga gcttcttcac ctttgcctgt
     3661 taaatctttt tcatttttat ttcttatctc tacaagctcg tccatatatc ctgtagtatc
     3721 caagactttt tctataagtt tgctaacagg ataaacttct tttatagttc taagagtccc
     3781 tataatatca acaaatccac ttatacttgc ccttgcctta gtagatatat ctgaatttgt
     3841 ttctatatca attaatactg agtatatact ttcctgtttt aagttagctc tatcttcaat
     3901 tttttctata gttctaagcc ctatacctct tctaggaaca tttataatcc tttttataga
     3961 tatatcatct tgtggatttt gaataactct taaatacgcc aacaaatcct ttatttcttt
     4021 tctctcatag aattttgtac caccatatat attgtatgga atttgacttc tatttaaagc
     4081 gtcctctaca ggtcttgcct gtgcatttgc tctataaagt actgcaaaat ccttataagg
     4141 tctattttct tttctagcta tttgtgcaat tgaatcagca atgaaatcag cctcttctat
     4201 ttcactacca gttaattgta tctttataag ttctccttct tttttttcac tccaaagctt
     4261 ttttcttttt ctttctatat tattagatat aactttatta gctgcatcta atataacttg
     4321 tgtagaccta taattttgtt ccaactttac aacatgtaca ttgtcataat ctttctcaaa
     4381 ttcaagtatg tttcttatat ctgcacctct ccatccataa atactttggt catcatctcc
     4441 aacaacacaa atattttgat gttctcttgc taataatttt atcaattcat attgagcttt
     4501 acttgtatct tggtactcat ctaccattat gtatctaaat ctacttctgt aatatgctaa
     4561 cacttcatca ttagccttga aaagttctac agttttaagt attaaatcat caaagtcaag
     4621 tgcactattt ctttttaaac ggtcttgata tagcgcataa atatctgcaa tcttaaccat
     4681 tctattatct gccatattga tatctttaaa ctgctttgga gtataaagtt tgtcttttgc
     4741 tcctgaaatt gcacttataa ctgcttttgg ttcaaacact ttatcactta aatttaattc
     4801 ttttagacag tctttaacca atgtaacttg gtctgctgaa tcataaatta caaaacttct
     4861 attgtaacca atcttattta tatcttttct tagtatacga acacagcaag aatggaatgt
     4921 acttatccac atatccttag tttcaggacc tatattttgt tcaactctct ctctcatttc
     4981 atttgcagct ttgtttgtaa atgttatagc taatatattt ggtgcttgta cacctttatc
     5041 ttctataaga tatgctattc ttgttgttaa taccttagtt ttacctgaac cagcaccagc
     5101 cagtataagt actggacctt cagtcttttc tactgcttct ctttgagcag gatttaatgt
     5161 atctaaattc atatttctcc tcctgttaac agccataaat atattttaat ctaaaatatg
     5221 aattacttca aattataatt atgtattata ttaatagatt atttccaata tattacaatt
     5281 aaataacatt tttccatata atttatttca ttgtttaata aaaaaaataa ccttatgatt
     5341 tggggaatca taaggcacct ttaaggggtc taggttaagg gttattatat ataaatctat
     5401 ttttttaaaa taggtttagg gcttaattat ttatttgtat taagttctct ttccttccaa
     5461 tacttataca aatcttcttc tttcttaaat aagggtaaat tcatatttct atgtactttt
     5521 actaaccatg gggattctag gccagcttgt ataatattat tttcatttat aaacacattt
     5581 tctgcttttc cttcatcaat caaaatcccc ttatccaata catatatgta atcacatatt
     5641 tcatacatta aattcatatc atgactggat attactattt ttatattatt tttatttaat
     5701 ccctttatta tatctacaat agacctagta cttactgggt ctaaaccagc tgtaggttca
     5761 tcaagtaata caatttcatt ttccattgct ataactgatg ctattgccac tctttttttc
     5821 tgtccatagc taagaaaatg tactggcctg tctataaaat ctattccatt aacagctact
     5881 agagctttgt ttattctctc tttaattgtt ttttcatcca taccaatgtt tctcattgca
     5941 aaggctatat catcatatac ttttgaataa aaaatttgtt tttctgggtc ttggaagaca
     6001 atacctacat tttttctaag gttgtataaa cttctcttat catattttaa cttttcttct
     6061 ttaaaaagaa tttctccaga tgtaggcttt aatataccca ttaaattcat aaataatgtt
     6121 gattttcctg aaccatttga acctattata cctataacat ttccctttga aaaatccata
     6181 ttaatgttta atagtgcatt ggtatttttt tcatattgat acgttaaatt atttatttta
     6241 aacatcatta tcccctacta tgtgaaattt accatcatac aattttatgt ctaatgatat
     6301 acacatatca tcataccttg tcataagtct tttgtacagc atacttccaa gaatacctaa
     6361 ggatttatat gaagttttta aatttatata tccaaatctt agctcttgtg acttgtatat
     6421 ttctgaaaat tcttctaaaa atatgaatat aaacctatat attaacattg ataactcgac
     6481 aaaagtgtca ggtaaatgaa gttttttaaa aaagaatatt aattgattaa aaggtgttgt
     6541 aagtatacaa aaatacacac aagtcaaaca agacatcgct ctaaataaca catgaataga
     6601 catgttaata gacgcctttg atattcctac attaaaacta aaaaattcaa aactatataa
     6661 taaatctgct ttattaaaag atatatttac aagagttatt cctatactaa gaaataaaaa
     6721 gtacattggt attctaagta attttaaata attttttaaa tctattccag ccacacaaac
     6781 aaccaaaata ctcattaaaa tcataattcc acttaaaata aatatatttt gtattaccat
     6841 agaagcaatc agaaaaatta ctcctatggc tacttttttg tttggattta ctttactcaa
     6901 tctatttgta tatgcatatt tatcaattat aagcatgatt aacttctctt tcctttttga
     6961 agacctaata tgtatcctat tacaccagcc cctaaagctg cttgtgcaga gaataataaa
     7021 ctttctattt ctccacttgg tggctcatat ggactgctaa accatggttt gtaatttgga
     7081 tttatcttag taatttcact ttcagcttga ccatctgctc ctccatattc agcacctgag
     7141 ttgacaagta atgggaatat aataagagct actactaata aaagtaataa tatgttagtt
     7201 tttgtttttg ttttagcact cattagcttt aactccttta tcattatttt ctagtatgct
     7261 tttttctccg ttttctgtaa ttaaattata tacaattaca gtaagtatac cttctgctat
     7321 tgctattggt atttgagtca taaagaaaat tcctaaaaat ttaattgctg aagccattac
     7381 cccacctgat gggtctggga aagccagtgc taattgtatt gaagttattg tatatgtggc
     7441 taagtctcct attgctgctg caagaaatac tggaatagca ttatttccat cttttttctt
     7501 taaagcctta aatattagga atgatacgat tggccctatt atcgccattg aaaaagcatt
     7561 agctcctaaa gtagttattc ctccatgtgc taataacaat gcttggaata ttaaaactat
     7621 agttccaaga acaaacatta cgcttggtcc aaacattatt gctccaagac ctacaccagt
     7681 aggatgtgaa caacttcctg ttactgaagg tatttttagt gctgataata caaacacaaa
     7741 tgctccacat aaagctaata atactttctt tttagggtct tgctttacta ttttacctat
     7801 tgattttaat ccgaataaga aaaatggtat aaatataact ccccatgcta tagaccattt
     7861 tactggtaag taaccttcca ttatatgcat tgcatttgat gtaactggag ttaaaatgat
     7921 taacataagt gcagctataa cccctaattt aatattctgt ttcatcttag tatccctctc
     7981 tccctttcta ataattaaaa cataataata catgtctgat ttatttaata aaataaaaaa
     8041 gcctaactcg atggttaggc ttctaatttt ccacaatcta aatacaaaca cataaaacaa
     8101 acatatctgt ataattatta taagaaactt tctccatccc cgaagaaatt actttaatat
     8161 tttaggcagg tctcctgact tagcttcatc ctactccttt tccttcccat gagatataaa
     8221 aaatatcatc atagtggcta acaaaagttt cgtctgcatc acagtagcgg gggctgtaaa
     8281 ggaatttaac ctttttccct attaatcttg cttaaagaac ctataatatt atatatttaa
     8341 tttttaaatt attcttaaat cattttctat ataaatcatt gtataccttt tctgtgaaaa
     8401 tttctacatt ttttataaat tttattcatt tttatatttt tatgtatata aattcttttt
     8461 tgtaagttta ttaatttcta attatcttct ttaaatacat tgaaactaca tcagaaaatc
     8521 tagttatatc tcttgaatat ataaaatcat ttccatatat taatctttca gcttcaagtt
     8581 ctgattcttt tcctgtaaat actcctaata ctaatatatt ttgctttcta gcttttctca
     8641 cttcattggc tgtatctctc aaaccaacta tacccctata agccttttct cctcttaatg
     8701 ttctctcact actttttcct atctttacat catttggtct tccatcgctg agaactatca
     8761 ggattttatt ttcttcatct ctatttatta gaccactaca tatagatttt attgccaaac
     8821 catctctatt attaccagca caaaaatatt caaatatatt ttcactattt tttatactat
     8881 catcataatc tttaaatctt tttaatattg tatagtctaa aaaactagaa aatcccatca
     8941 ctctatttgg tatgccaact tctgttagag catttgcaat tatgtaagct tgaattgcca
     9001 catttccctg atttctactt tgagagccac ttgcatccaa taatatatca ataacgtatt
     9061 tccctttttc attttctata tctttataaa atattttatt attattactt ctaccaactc
     9121 tccaagctct attggctaat atagtaccac aatcagaata tactctactt ttagcatttt
     9181 cttctattaa aattcttgac agactttctt ttaatctctt tatttgctgt ctatacattc
     9241 tagcatgttc tctaaatttt cctaaattat tttctttttg tcttgttaca tattttagtt
     9301 gaaatacatt attacactca cttctaagaa ctccatctgt aaagtgtatt ctacaacctt
     9361 catgaacatt tctacaattt tgtgtctcaa tttttttaat ttcaccttta ctcaaataag
     9421 tttttccata ataatgttct atttttgaat atatcttatt tgcagtgtct tcatcaacat
     9481 atataactcg gtctgctgaa ttgctacatt tagaatcatc aaaatcccct acaccttcta
     9541 caagcatgga tgaagatatt gaatctatat cactttcaat aatattttct tcttcatcac
     9601 tataaagttc ttcatacatg aaatcagcaa atgtatcaaa atttacttct acactttcta
     9661 cattattttc atcaataaga ctattttctt ctccatttaa tatgtattca aaataagtta
     9721 aatatataac atctaatgct tttaaaatct ccataatatc ttgattggta tctatatttc
     9781 ttatatcatt aactattctt ctagtcatac tatctaatgc tgggtgcttg tccatactat
     9841 aaagtacaaa tgtgtattta attttatcca taatattatc attgtgtatt ttggtaaaac
     9901 gtgaaacaat atctttataa gcattccttc ttatgtcttc tatccctggc ctgtctttaa
     9961 tgaccttttc ttcaactatt gtatttaata taacttgaat tagacctagt ataacatctt
    10021 tgttgtatcc ttttcttatt ctatttgtaa gatatttttt aactatatcc caatctacat
    10081 actttcttct agcacctgca attattgcga aatatagacc tatttctttt gaagagtaat
    10141 ctttttctga tatatctata ctttcattgt aatcaccagc tattgtccat gataggttat
    10201 tagctctatt ttcaaattca taatcatcat atatccattt cataatctca cctatattaa
    10261 atatgactta ttggaaacac atctatactc tcccatttat taggtattct agttttaatt
    10321 acatctccaa ccatttcttt ctcatataca tcaaaagtct ttcctgtaag tcccatatta
    10381 attgcaagag ttggttttaa tcctcttctt atagtcttaa gagatgccat aagaccacgt
    10441 aaatcaatag ctttactaga tatctctcca ttttgagact tcaattgtaa atctaaaaat
    10501 attcctgcaa agtgtattaa tttttcttca tcagcatctg aaaattcatt ttttaaaatc
    10561 atcattaact tatcttcttc tataggagga atatctatta ccataaatct agataccagt
    10621 gcttcattaa gttcttttgt tcctgcatat tcataattca tagtcccaat aaatcttgtt
    10681 gctggatgta gattgactct ctcataccca ggtacatcaa ttattcttct atagtctaaa
    10741 gctgaatgta ccacaactat agcatcattt tttgccatat taatttcatc aaacacccca
    10801 aaacctccat taatagcaca ctcataaact gagcctcttc taagtttaac ttcattgtct
    10861 ataaatgtat ctgtacctat aagtgttgaa ctgtctgtat ttatatgaaa tgatgtattc
    10921 cactgaggtc tattaaatat ttcccctaga ttatcagcaa gtaagttttt tcctgttgcc
    10981 tttggtcccg ataataatat attttcttct tctaaaatag ctgctataca catagacaag
    11041 atatctttac cataaaaata agttttagac tttgttactc tgtattctaa atctttttct
    11101 aaaccataat aatttctaaa atacagtaca tcttcaataa gtttttcatc taccccttgc
    11161 acccttagat tatctattat atccatattt ttaccccttt tatataaata gtatttgttt
    11221 catttcttat caccatttta gcataaaagg atataagcta aaattgattt tttaatctcc
    11281 ccattttaag taaaaccatg caccatttta tcactacaaa attaagagta ctacttactg
    11341 atatttcttg attttgttcg tctatacgta tataatatat gctgataaat tatgaggaaa
    11401 ggatgattga aatatggatg cccagaagta gcaaaaaaat ggggtatttc tgaaagaaga
    11461 gtacaaaaac tatgtgaaga aagtcgcata ccaggagttt caaaactcgg gtatatgtgg
    11521 ctgataccaa agaacgcaga aaagcctgtt gatggtagaa caaaaagaag aaaggaagta
    11581 caatatgaat agcgttttga ttatagatga tgacaaagaa ctttgtgctc ttatgaaaaa
    11641 atgtgttgag caagaaaatt tatctgctat tactgcacat tgtggtgcag aaggtctaca
    11701 tttggtcgat aaaaatagac acacactctc tcttgtaatt ttggacataa tgatgccaga
    11761 tatagacggt tttcaagttc tgaaaaaaat tagagaaaca agtaatattc cagtaattat
    11821 gcttactgct aaaaatgacg aggatgataa agtttccgga ctacgtttag gtgctgacga
    11881 ttacctaaca aaaccgttca gcataaacga acttatggct cgtgtcaatt ctcttattcg
    11941 tcggtatact atgttaaacc ctgcttttat aacagacaca gattgcataa taataaatgg
    12001 catgattatt gacaagacaa accgaattgt ttctgtcaat aatattcctg ttcaacttac
    12061 aagcaaagaa tttgatttac ttgcttttct ggcttctaat aagggacggg tgtttaccaa
    12121 aaaacagatt tatacacaag tatgggaaga agaatatgct tttgacgata gtaacatcat
    12181 gtctttcatc agtaaattga gaaaaaagat tgaaccagac cccaaccatc cattttatat
    12241 tttgacagtt cgtggtgtcg gttatcgttt taataaggag gcttaacatg aatacaagtg
    12301 tctatctgct tttgatattg gttcttagcc tgattattat agcgtacctt gtttctgtcc
    12361 tgctacgtgt ccgaatacaa cttacactta taaaagatgc tttagaggat ataaaaaatg
    12421 gaaaccttaa tcgacgtatt ttaacagatg aaaacgacat tacaaaacaa atttgctatg
    12481 atattaacga aatagcaatc aatagccaat ttcaacttat tcagcaaaaa caatcagaac
    12541 aagcctacaa acagctaatg acaagtctct cccatgatgt aaaaactcca cttgcttctt
    12601 tggtcggtta tttggaagca gtagaaagta aaattgttgt aggagaggaa aaagacgagt
    12661 atatccatgt tgcgtctaat aaggctcatt acttaaagaa ttttgtagaa aatctatttg
    12721 aatgggtaaa attggattct aaggaacaag tttttcattt tgatattttt gacttgaatg
    12781 aattatcccg taatattata tctgattgga tacctgtttt agaaagcagt cattttgaat
    12841 atgaatttga tataccagaa atagagtact tcttacgtat agacgcaaat gcatataccc
    12901 gtatcattaa taacctatta caaaatatta ttacccatag tagtggcaac aaaatgacat
    12961 tgagaatttt tgaaaacaaa gagcaagctc aaattataat aacagataat gggaaaggta
    13021 tatcttcaga caatctccca catatttttg aaaggttgta ccaatgcgac cattcacgag
    13081 cttcaaaggg aaatggttta ggcttggcga ttgcaaagga acttattaac gctcataagg
    13141 gaaatattac cgccaacagc actcccggta tgggaacaga atttacaata ttgcttccta
    13201 aagccctgta aaagtacgag gctttcttac tctaaaacaa gataaaagca agattatggc
    13261 aaggttttgg caaggttcat gttttataat tctaattgta gttgattttg acgttgtatc
    13321 aactatcttt tgtagaaaca aatttattaa aagtgaggaa attatatatg aatgattttg
    13381 tgattgaaac aaagcaactt acaaaaattt atgggaatca aacagtagtt gacaaagtca
    13441 atctccatgc aaaaaaaggc cgaatatatg gtctgttagg acgcaacgga gctgggaaaa
    13501 ccacaattat gaaaatgatt ttaggattga cttcaatcac gtatggcgag gtagatgtat
    13561 ttggagagaa tattaaggga cgtgaaaaac aagtatatcc ccgtatcggt gcaattatcg
    13621 aaacgccagg attttatcct aatctgacag gaacggagaa cttggagatt tttgcaaaac
    13681 tgcgtggtac agccgcccct aattctgtaa aaagagcatt ggaaattgta ggcttacctt
    13741 ataaaaataa aaagctgttt agtgaatatt cacttggcat gaaacagcga cttggtattg
    13801 ccaacgctat cctgcatgac ccagaactgt tagttttaga tgagccaacc aatggtcttg
    13861 accctattgg tattgcggag atgagaaagt ttattaggga cttgagcgtt gagcatggaa
    13921 agacaattct tatttccagt catattcttt cagaaatctc attgttggct gatgatattg
    13981 gaattatcga ccacggtgtt cttttggaag aaagtagtat gagtgaacta gaaaagaaaa
    14041 acagtaaata tatcctgcta caagtttcag atattccgaa gacatcactt attttagagc
    14101 gtcaatttaa cacgaaggat tattccgttc aagatgacta cacgttacac ctttataaca
    14161 ctaatctgga tatgggtgaa atcaataaag cacttgtaat gcagaatgta actgttatta
    14221 gttctcaact ttgtaatgat actttagagg actactttaa aaaaatcaca ggaggagagg
    14281 gcattgctta aacttattca aacagaattt cttaaactgc gtcgtaggaa acttatttgg
    14341 ctgatgttgt tgtctgctct cattatgccg tttttcgcac tactatactt caattatttt
    14401 ggtaaaacag gtgttgagcc aataaagttt tataaatggt ctgcatttgg ttatacactt
    14461 tggattattt tgcctgttgt tttgggtata ttttgcacca tgcttataca cgaagaaaac
    14521 cagtatgata tgctcaaaca gctttggatt gtacctatta gtaaaatggg atactttttt
    14581 agtaaatttt tcgttgtttt gatttactct atttgtttta tgctgattac agccgttgct
    14641 tctgtgttat ttagtgtact ttctggatat gtcgtattta gctgggcaag cattttatat
    14701 ttgttgaaaa aatgtttaga aattggtgta atcactgctt ttgtaatgtt gccgattttg
    14761 gcaatcgcta catctcaaaa aggatacatt ctcccaattt gtatcacatt ggtatatgcc
    14821 ttttccggtt ttgttataat gacaataaat atgtatttac acccattatc cagtatggga
    14881 gttattatca tgagaaataa agacattcca ggtcttatat tttcacagga aattaatgtc
    14941 ccattagcat ttctttgtat ttgtatttgg agtattgtat cagtattgtt tgcaaacact
    15001 gcactaggaa gaagaaagtg aggtgtagat aatgtggaaa ttactttggg ctgaatgtca
    15061 aaaaattcgt cgttcaaaaa ttgtttggat tacagttttt gcatccatta tgatagcagt
    15121 catagttttt gcaggagggc aggatgtata taatgaccca gatttacatt atggcttaaa
    15181 gagcgtttat gacggttcac ggtatataga caatgcaggt tggtatatgg acgcagtaca
    15241 accttggtca actttttttg ttcttcctgc tgtcattgcc ctattaggaa gctatattat
    15301 ttgtcgtgaa gaagaggagg acactattaa atccctgcgt cttattccca taaatgaaac
    15361 aaaattaacc atagcaaaaa tgattattac ttttatattc agtatacttc tttatttgtt
    15421 actctttact ataacatttt tgactgaatc cgttttacat ttttccgatt tatcgacaaa
    15481 actggtttta agttgcatga aagagtattt tcttgatggc ataggtgtat tctttgcaat
    15541 ttctcccatt attgccttgg tatcgcgcat gaaaaaagga tattggcttg cattagtatt
    15601 cacagaagtt tattctattg ctggactatt tgcgggtatg tcaaatgtct tacaaacctt
    15661 ttttcctatt gcggcaatat ttaatatttc tggatactat ataactactg gagaaaaaat
    15721 tatggggagt atcataagtt tattactttg tgcctgcctt tctgcattta tacttaaagg
    15781 gctaaaacac aatgaaaaaa attgaaatag gtatatcaaa atgaaaaaga taaaaaaact
    15841 gattattcta attgtattct tgtttctttt tggcatgtta cactgttata ctgaatggaa
    15901 attcaacaat aacccaccta aaggggtaat agaaagaata aatgaatgga ctgataagat
    15961 tggactggat tagtaactta tgttaattat agtgaatgta atattagcaa gttattgttt
    16021 cgtattttaa tactataact ttaaatattt ggttttaatc ttccaaacga atataaaaaa
    16081 actgtcacag aggcaacata tttttgaata tttataaagt ggtagttttg attttcaaag
    16141 ctgccgcctt ttcttctata taagcaaact tatcctcaac aaagtgtaaa acatggtaca
    16201 ttttaagaaa aaagtaccac agacacaagc aataaaagaa acgccgagaa cctacaaaaa
    16261 cgtagtgctt atgcagattt acgacgcctt ctattttctc aaccgtctta atgaccttgt
    16321 acaaaagaaa tttttctaat attttttata atcttatctt gtgatgtttt ttatgtataa
    16381 atgacttaat aacaaatcca ataaaaatta tggatattgg tataattaga acgaggaatt
    16441 tataatcatt ataatgtgac attattctta cccaactctc agaaaaaata tatcctatac
    16501 tgattaatga agtattccat atggttattc ccacgataga tgatattaca aaagttataa
    16561 tattttgacc tgctatacct gctataaaag atatgtatgt tctacataaa gggattaatc
    16621 tacatacaca aacagaaata gaagagtatt tgttaaaata atcttgagat gcaaatattc
    16681 ctttctttgc ctttggacat atctctatta ttttatttat gatagattta cctccccaat
    16741 ttcccactgt ataacaaaat atcgaaccaa gtatacctgc aattatactt aaaattaaaa
    16801 ttgtcagaaa tccaaaatga tttcttgcag caattgctcc acataaaggt aaaacaactt
    16861 cgctaggtaa tggaaaacaa gcatactcaa tcataattaa tacaaataca gagattaatc
    16921 catattgtaa aataaaacta ttgattacag acacttactt atctatctcc tttcttattt
    16981 aaaggtttta agggtacatg ttctaaggat tctcacccta tatttaaaga atcaatctct
    17041 ttaaactatt atatgtattt acacaaagca tttataacat actttaaaaa tagtacctta
    17101 aaataagaaa cttaataaat aaaaaaa
//
LOCUS       DAYAOY010000093        16496 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYAOY010000093 DAYAOY010000000
VERSION     DAYAOY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.093"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..516
                     /locus_tag="ACGI6B_003054"
     CDS             <1..516
                     /locus_tag="ACGI6B_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFG9752471.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGVVATIIE"
     gene            766..915
                     /gene="rpmG"
                     /locus_tag="ACGI6B_003055"
     CDS             766..915
                     /gene="rpmG"
                     /locus_tag="ACGI6B_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFG9752472.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            941..1162
                     /gene="secE"
                     /locus_tag="ACGI6B_003056"
     CDS             941..1162
                     /gene="secE"
                     /locus_tag="ACGI6B_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFG9752473.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1182..1724
                     /gene="nusG"
                     /locus_tag="ACGI6B_003057"
     CDS             1182..1724
                     /gene="nusG"
                     /locus_tag="ACGI6B_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFG9752474.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1763..2188
                     /gene="rplK"
                     /locus_tag="ACGI6B_003058"
     CDS             1763..2188
                     /gene="rplK"
                     /locus_tag="ACGI6B_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFG9752475.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2259..2957
                     /gene="rplA"
                     /locus_tag="ACGI6B_003059"
     CDS             2259..2957
                     /gene="rplA"
                     /locus_tag="ACGI6B_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFG9752476.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    3009..3137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3180..3686
                     /gene="rplJ"
                     /locus_tag="ACGI6B_003060"
     CDS             3180..3686
                     /gene="rplJ"
                     /locus_tag="ACGI6B_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFG9752477.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3744..4109
                     /gene="rplL"
                     /locus_tag="ACGI6B_003061"
     CDS             3744..4109
                     /gene="rplL"
                     /locus_tag="ACGI6B_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFG9752478.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4500..5288
                     /locus_tag="ACGI6B_003062"
     CDS             4500..5288
                     /locus_tag="ACGI6B_003062"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFG9752479.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5759..9475
                     /gene="rpoB"
                     /locus_tag="ACGI6B_003063"
     CDS             5759..9475
                     /gene="rpoB"
                     /locus_tag="ACGI6B_003063"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFG9752480.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9517..13002
                     /gene="rpoC"
                     /locus_tag="ACGI6B_003064"
     CDS             9517..13002
                     /gene="rpoC"
                     /locus_tag="ACGI6B_003064"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFG9752481.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13291..13713
                     /gene="rpsL"
                     /locus_tag="ACGI6B_003065"
     CDS             13291..13713
                     /gene="rpsL"
                     /locus_tag="ACGI6B_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFG9752482.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13837..14307
                     /gene="rpsG"
                     /locus_tag="ACGI6B_003066"
     CDS             13837..14307
                     /gene="rpsG"
                     /locus_tag="ACGI6B_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFG9752483.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14356..16422
                     /gene="fusA"
                     /locus_tag="ACGI6B_003067"
     CDS             14356..16422
                     /gene="fusA"
                     /locus_tag="ACGI6B_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFG9752484.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6479 a         2052 c         3377 g         4588 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttgttgc tacaataata gagtaattct ttactaaact gatggattaa
      541 aaggaataac aaaaagaggg gttatgctcc tcttttttta acatagaaat acaaaaacat
      601 tgacacagca taggattttg tgataactta tacaaggtaa tgattatagg aagttaataa
      661 tatcttgaat actgtagatt tttaataaat acatatttta aagatattat attcacatat
      721 attaacaata tatattattg tattacaaag ttggaggtgt atcagatgag agttaaagta
      781 actttagcat gtacagagtg taagcaaaga aactacaaca ctactaagaa taagaaaaat
      841 aatccagaca gaatagaatt acaaaaatat tgtagattct gtaaaaagca tacaactcat
      901 aaagaaacaa aatagttttt tataaggatg tgagtatgga atggctgccc aaaaaaatga
      961 cggtgcaaaa actaaaaaaa ggtttagttt gtttggatac ttaaaagaaa ctaaacaaga
     1021 attaaaaaga gttacatggc cgacaaaaaa ggaactgttt aaaaacacaa gtatagtttt
     1081 aactgtagtt atttcatgca ctatattagt atggggtata gacactatat tatctggtgc
     1141 actagcgtta ttactaaaat agacgaaaag aggtaagtta aatgtcagaa ttacaagaag
     1201 caagttggta tgtagttcat acctattcgg gacatgaaaa taaagttaaa gctacaattg
     1261 agaaagcagt taaaacaaga ggtatggaag actgcataag acaagtagtt gttccaacag
     1321 aagaagtagt ggaaactact aaaactggaa aagaaaaaac tagacaacgt aaagtttatc
     1381 caagttatgt tttggttaaa atgataataa ctgatgaatc ttggtatgta gtaagaaata
     1441 ctaagggagt tacaggtttc gttggacctg gttcaaaacc agtgccatta agtgaagacg
     1501 aagtcaaagc tatggggata gacacaacag accctaaagt agtaaatagc gatattgatt
     1561 ttgaaatagg agatacagta aaagtatctc aaggaccatt tagtggacaa ataggtaaca
     1621 tagaagaaat tgacttagaa aatagagaag tcaaagtatg cataaatgca tttggtaaaa
     1681 ggactctatt tgtaatagag cttgaaggta tagaaaaaat ctaatactat attatagaaa
     1741 aatctgagga ggtgcgctca aaatggctaa aaaagttata ggtcaaataa aattacaaat
     1801 acctgcagga aaggctactc cagcgccacc agttggacca gcattaggac aacatggtgt
     1861 taatataatg ggattcacaa aagaatttaa tgctaaaact gcagatcaag ctggaatgat
     1921 aataccagtt gttataactg tatatcaaga tagatcattc agttttataa caaaaactcc
     1981 accagctgca gttttaatta aaaaagcatt aaacttaaaa tcaggttctg gagaaccaaa
     2041 caagaaaaaa gttgctaaaa tgacttcagc tcaagtaaga gaaatagctg aattaaagat
     2101 gcctgactta aatgctgcat cagtagaagc tgctatgagt atgatagctg gtactgctag
     2161 aagcatgggt gttgtaatcg aagactaatt aaaagataca ctttttttta taaagtggga
     2221 ggctagaagc cgaaattaac cacgaggagg aaaataaaat ggctaaaaaa ggtaaaagat
     2281 atgcaggtgc attacaaaaa gtagatagaa ctaaatttta tgatgcatca gaagcattaa
     2341 cattagtttc agatatagct ggagctaaat ttgatgagac agtagaagca cacattaaat
     2401 taggtgttga ctcaagacat gctgaccaac aagtaagagg tgcagttgta ttacctcatg
     2461 gaactggtaa aacaaaaaga gttttagttt ttgctaaagg tgaaaaagct aaagaagctg
     2521 aacaagctgg tgctgacttt gtaggagctg aagaattagt tcaaaaaata caaggtgaaa
     2581 actggtttga ctttgatata gtagttgcta ctccagatat gatgggtgta gtaggtagat
     2641 taggtagagt attaggacct aaaggtttaa tgccaaaccc taaatcagga acagttacat
     2701 ttgatgtagc taaagctata gatgaaataa aagctggtaa agttgaatac agattagaca
     2761 aaactaacat aatacacgtt ccagtaggaa aagtatcatt tggtggagaa aaattaactg
     2821 aaaactttac tgcattaatg gatgctataa taaaagctaa accagctgct gctaaaggac
     2881 aatatttaag aagtataaca gtagcttctt caatgggacc tggagttaaa ataaacccag
     2941 caagaacagc tgaataataa aactgttgac acaggttgac aaaaatgtta ttatatcact
     3001 tgtcaatgta aaaaagaata gatttgccgt agatagtggg tgcgaaagct taatatcccg
     3061 ccgaggtttt agataaattg agattactaa agcttttgat gtctactttt ggcaacaaaa
     3121 gctttttata atgttgcggt aaatactaag cctatttggt gaaggaggtg cacatctata
     3181 tgagaaaagc tatagaaatt aaatcagaag ttgtttctga gatagttgaa aagttacaaa
     3241 aatcttctgc tgctgttgtt gtagattaca aaggattaac agttgaagaa gtaactgagt
     3301 taagaaaaca aatgagagaa gctggtgtag attacaaagt atacaaaaat actttagtta
     3361 gaagagcagc taaagaagtt ggtatagagc aatttaatga tgaattatta gttggaacta
     3421 atgccatagc atttggatat gatgatccag ttgctccagc tagaatatta aaaggattca
     3481 tggattctca tccaaaaatg aaattaaaga tgggtattgt ggaaggtgca ttctatgatg
     3541 aatctaagat tgttgaaatg gctaacatac catcaagaga agttcttatc gctaaattac
     3601 ttggtagctt aaaagctcca gtatcaaact tcgcatactt aatagatgca atagcgaaaa
     3661 aggcagaagg tcaagaagaa gcttaattgc ttaactaaaa aattataaat attaatttga
     3721 aataaaattc ggaggtgcta aaaatgacaa tagaacaaat attagaagct atagaaaata
     3781 tgaaagtttt agaattaaat gaattagtta aagctgctga agaaaaattc ggtgtatcag
     3841 cttcagctcc agtaatggta gctggtgcag ctgctggtgg accagctgct gaagaaaaaa
     3901 ctgagtttga tgtagtatta actgacgttg gttcttcaaa agttggagtt ataaaagcag
     3961 ttagagaaat aacaggatta ggattaaaag aagctaaaga agtagttgac aatgctccta
     4021 agacagttaa agaaggagct tctaaagaag aagctgatca aataaaagag aaattagaag
     4081 ctgctggagc taaagtagaa gttaagtagt ttctttaaaa agatacccat tgggtatctt
     4141 tttttattta taaaaaacta taaagaaagt atttggaatt ataaagaaag tatttttata
     4201 aaataaaaaa aatttatata atagtactaa attaagggta tcatatatgg tgtaatggta
     4261 ttttaattta acattattat caaattaaaa tataaacaat tgttgtaatg tctgtatata
     4321 aagtatataa ttaaactaaa atgtaaaatt ttacatttta gtttaaaaaa atacacgagt
     4381 gtttataaat aaaatgtaat aaataattaa ttaacaaata taaattttaa aataagttag
     4441 atatttagaa aaatcgaaat ttgtaggatt taaaggacat attcaaggag gagttaataa
     4501 tggaaaaatt acaaggaaaa attgcagtag ttactgcagc aacaaaaggt attggattag
     4561 catcagcaga gatattagca aaaaatggag caactgtgta cttagcagct cgttcagaag
     4621 aattagctaa tgaagttata aataaaataa gtgcagaagg tggttgtgct aagtttgttt
     4681 actttaatgc tcgtgaagaa gagaccttta cctcaatgat agaagaggta gttaaaaaag
     4741 aaggtaagat agatatactt gtaaacaatt ttggttcaac aaacccttct cttgacaaag
     4801 accttgtaac tggggataca gataatttct ttgacacagt aaatactaat ttaaaaagtg
     4861 tgtatttacc atgtaaagca gcaattcctc atatgataaa gaatggaaaa ggaagtatag
     4921 taaatatatc aagtataggt tcagtattac ctgatttatc tagaatagct tactgtgtat
     4981 caaaagcagc aatcaactca ttaactcaaa acatagctac acaatatgct aaagacaatg
     5041 ttagatgtaa tgctgtactt ccagggctta ttgcaactaa agcagcatta gacaatatgt
     5101 ctccagagtt cataaaagaa tttttaaagc atgttccttt aaatcgtata ggggaaccag
     5161 atgatatagc aaaagctgtt ctattctatg ctagtgatga ctcatcattt ataacagggg
     5221 atttacttga agttgcagga ggatttggtt tacctactcc acaatttgca gataatatat
     5281 taggataatt aaattactaa tatgcaatga aagcttatgc attagttata gtagaaatga
     5341 tatttttata aaggccatag atataattta tattggaaag gtttgaggtt caaaattcca
     5401 agttaaattt atttatggtc tttattttgt atattttaat aaattgtata tatttgtata
     5461 aatgtaatta tttacaatca ttttaatatg gaattataat taaatagggg ggatggtact
     5521 tgtataaaat tttttataga aaattagacc tataaatgaa aaatatggat tttatattgg
     5581 aaaattgtac ataatacata atatatgctt aaaataattt acaaaaaatc aaaaataatg
     5641 catagtttac atataagata aaaaatatat tgaaaaatac ttgacattat gctaaaatta
     5701 ttagatgcgt tagaatgaac tattgtttta tttttaatta ttgagaggtg aaaagtgaat
     5761 gccacatcct gtcacgatag gtaaaagaac tagaatgagc ttttctaaaa ttaaggaaat
     5821 agctgatgtg ccaaatctta tagaaattca agtagattcc tatgagtggt ttttaaaaga
     5881 aggtttaaaa gaagtgtttg atgatatttc accaatagaa gattatactg gtaatcttat
     5941 attggaattt gtcgactatt ctctagatga taaaccaaag tacgatatag aagagtgtaa
     6001 agagagagat gctacatatt gtgcacctct aaaggttaag gtaagactta taaataaaga
     6061 aacaggtgag ataaaagaac aagaagtgtt tatgggtgac ttccctttaa tgacagaaag
     6121 aggaactttc gttataaatg gagcagaaag agttatagta agtcaattag ttagatctcc
     6181 tggtgtttac tatgcggaag aaagagataa aacaggtaag agattgattt catctactgt
     6241 aattcctaat agaggagcat ggttagaata tgaaacagat tctaatgatg taatatctgt
     6301 aagagttgat agaacaagaa aacaaccagt tacagtttta cttagagctt taggaatagg
     6361 aacagatgca gagataattg accttttagg agaagatgag agattatctg ctacattaga
     6421 aaaagataat actaagacag tagaagaagg tcttgtagag atatacaaaa aattaagacc
     6481 aggtgaacct cctactgtag aaagtgcatc atctttatta aatgctttat tctttgaccc
     6541 aaaaagatat gacctagcaa aagttggtag atataaattc aataagaaac ttgctttatg
     6601 ttatagaata atgaataaaa tttcagcaga agacataata aatccagaaa ctggagaagt
     6661 atttgtaaaa gctggagaaa aaataagcta tgatttggct aaagccatac aaaatgcagg
     6721 tataaatgta gtaaatctac taatggatga tgataaaaaa gttagagtta taggaaataa
     6781 ttttgtggat attaagtctc acattgattt tgacatagat gatttaaaca taaaagaaaa
     6841 agtacattat ccaactttaa aagaaatttt agatggatat agtgatgaag aagaaataaa
     6901 agaggctata aaatcaagaa ttaaagaact tataccaaaa catatattat tagatgatat
     6961 aatagcttca ataagttatg agttcaatat attctataat ataggaaata ttgatgatat
     7021 agatcaccta ggaaacagaa gaataagatc tgtaggtgaa ttgttacaaa atcaagttag
     7081 aattggtctt tcaagaatgg aaagagttat aaaagaaaga atgacggttc aagatatgga
     7141 agctataacg cctcaagcgt tagttaacat aagaccagtt tcagctgcaa taaaagaatt
     7201 ttttggtagt tctcagttat ctcagttcat ggatcaaaca aatcctctat ctgagttaac
     7261 gcataagaga agattatcag cccttgggcc tggaggtctt tcaagagaaa gagctggatt
     7321 cgaagtgcgt gacgttcacc attcacacta tggtagaatg tgtccaatag agactccaga
     7381 gggaccaaat atcggtctta taaactctct aggaacttat gcaaaaataa atgaatttgg
     7441 atttatagaa tcaccatata gaaaatttga taaagaaaca tcaacagtta ctgatgaaat
     7501 acattattta actgctgacg aagaagattt attcgtaaga gctcaggcaa atgagccatt
     7561 aacagaagat ggtaaattcg taaaccatag agttgtttgt agaactgtaa atggtgctgt
     7621 tgaaatggtt cctgaaagta gagtagatta catggatata tctcctaagc aggttgtatc
     7681 tgttgccact gctatgatac ctttcttaga aaatgatgat gccaaccgtg cccttatggg
     7741 agcgaacatg cagcgtcagg cagtgcctct agttagaaga gaagcaccaa ttataggaac
     7801 tggtatagaa tacagagcag caaaagactc tggagcagtt gttgttgcta gaaattctgg
     7861 tatagcagaa agagtaacag cagatgaaat aattataaaa agagaagatg gaaacagaga
     7921 tagatataat ctacttaagt ttaaacgttc aaactcaggt acttgtataa atcaaacacc
     7981 tataataaat aagggtgacc agataataaa aggtgatgtt atagcagatg gtccagcaac
     8041 tgatttagga gaagttgcac ttggaagaaa ctgtcttata gcattcatga cttgggaagg
     8101 ttataactac gaggatgcta tattaataaa tgaaagatta gttaaagaag atagattatc
     8161 aacaattcat atagaagaat atgaatgtga agctagagat acaaaactag ggccagagga
     8221 aataactaga gatataccta atgttggaga tagtgcaatt aagaacttag atgacagagg
     8281 tataataaga ataggtgcag aagtagactc aggtgatata ttagttggta aagtaactcc
     8341 aaaaggagaa actgaactta ctgctgagga aagattactt cgtgcaatat ttggagaaaa
     8401 ggctagagaa gttagagata cttcacttaa agtgcctcat ggtgaatctg gtataatagt
     8461 tgatgtaaaa gtatttacta gagaaaatgg agatgattta tctccaggag taaatgaatt
     8521 agtaagatgc tatatagcta aaaagagaaa aataaaagtt ggagataaaa tggctggtcg
     8581 tcatggtaat aaaggggtta tctcaagagt attacctgaa gaagatatgc cattcatgga
     8641 aaatggaaca ccactagaca taatacttaa cccacaaggt ataccatcac gtatgaacat
     8701 tggtcaggtt ttagaagttc atttaggact tgctgctaag acattaggat ggtatgtagc
     8761 aacatcagta ttcgatggtg ctaatgagta tgacataatg gatgctcttg aagaagctgg
     8821 gtatcctaga gatggtaaat taactttata tgatggtaga acaggtgagt cttttgacaa
     8881 cagaataact gttggatata tgtactactt gaaactacat cacttagtag atgaaaaact
     8941 acatgcaaga agtactggac catactcatt agttactcaa cagccactag gtggtaaagc
     9001 acaatttggt ggacaaagat ttggagagat ggaggtttgg gctctggaag catatggagc
     9061 tgctcacata cttcaagaaa tacttactgt taagtctgat gacgttgtag gtcgtgttag
     9121 aacatatgaa gctatagtta aaggtgaaaa tatacctgaa ccaggaatcc cagaatcatt
     9181 taaggttctt ataaaagaac ttcaaagttt atgcttagat gtaaaagtat tgacagatga
     9241 agaccaagaa atagaagtaa gagaatctgt ggatgaagat gatacaatag gtgaatttga
     9301 gctagatgtt gtaaatcata tgggagaagt tgaagaaagt aatataatag aagaaattga
     9361 agatgatttt gcagaaaatg cagaagatga ggatatagag aatttagaag aattcactga
     9421 agatgattta tttgaagaag aaatagattt tgatagtgat gactttgata tgtaaattta
     9481 attaatgtaa atctcaaata gaagggagag aactccttgt ttgaattaaa caatttcgag
     9541 tcgataaaaa tagcattggc ttctccagaa aaaataagac aatggtctag gggagaagtt
     9601 aaaaagccag aaactataaa ttaccgtact ttaaaaccag aaaaagatgg tcttttctgt
     9661 gaaagaatat ttggaccaca aaaagactgg gagtgccact gtggtaaata tagaagagtt
     9721 agatataaag gtgtagtttg tgatagatgt ggagtagaag taactaaatc aaaagtaaga
     9781 agagagagaa tgggacatat agagctagct gctcctatgt ctcacatctg gtacttcaaa
     9841 ggtataccaa gtagaatggg acttttactt gatatgtcac caagatcgtt agaaaaaata
     9901 ttatactttg cctcatatgt ggtagttgat ccaggagaga ctggattaaa cgaaaaacaa
     9961 ttgcttacag aaaaagaata cagaactgct cttgaaaagt atggatatac ttttactgta
    10021 ggaatgggtg ctgaagctgt aaaaacatta ctacaaaata tagatttaga gcaacaaagt
    10081 aaagacctaa gagcggagtt aaaagatagt acagggcaaa agaaagttag aacaataaga
    10141 agattagaag ttgtagaggc atttaaaaag tctggaaata aaccagaatg gatgatttta
    10201 gatgcaatac cagtaatacc accagattta agaccaatgg tacaacttga tggtggaaga
    10261 tttgcgactt cagacctaaa tgatttatat agaagagtta taaatagaaa caacagactt
    10321 aaaagattat tagagcttgg agctccagat ataattgtaa gaaatgaaaa aagaatgctt
    10381 caagaagctg ttgatgcatt gatagataat ggtagaagag gtagacctgt aacaggacct
    10441 ggaaatagac cacttaaatc tttatcagat atgttaaaag gtaagcaagg tcgtttccgt
    10501 caaaacttac ttggtaagcg tgttgactac tcaggacgtt ctgttatagt tgttggacca
    10561 gaacttaaat tttatcaatg tggacttcca aagaagatgg cattagaatt attcaagcca
    10621 tttgttatgg ataaattagt taaagaaggt tatgcacata atataaaaag tgcgaaatct
    10681 atagtagaaa aagttaagcc agaagtttgg gatgttttag aagatgttat aaaaagtcat
    10741 ccagttcttc ttaaccgtgc accgactcta catagattag gtatacaagc atttgaacca
    10801 atcttagttg aaggtaaggc tataaaacta catcctcttg tatgtacagc ttataatgca
    10861 gactttgatg gtgaccaaat ggcagttcat gtacctttat cagtagaagc acaagcagaa
    10921 gcaagattct taatgctttc tgtaaataat atacttgctc ctaaagatgg ttcacctata
    10981 actactccat ctcaggatat ggttttaggt tgctattatc taacaataga agcccaagat
    11041 ggagctaaag gaacaggtat ggtatttaaa gactttaatg aactattact tgcttattac
    11101 aataaatcag ttcatctaca tgcattagtg aaacttaagg taacacttga agatggaaga
    11161 agttcattgg ttgaaagtac tgttggtaga tttatattta atgaaaatat acctcaagac
    11221 ttaggttttg tagatagaaa agaaaatcca tttgcacttg aagttgattt cttagcagat
    11281 aaaaaatctc ttggtaaaat aatagataaa tgctttagaa aacacggaaa tacagaaaca
    11341 gctgaattac tagattatat aaaagctcta ggatttaaat attctacatt aggtggcata
    11401 acagttgctg ttgatgatat gagtgtccca gaagaaaaga aagtatttat agctgaggca
    11461 gaagctaaag ttgataagta tgaaaaagca tacagaagag gtctaatctc tgatgaagaa
    11521 agatatgaaa aagttataga gacatggaca gaaacaactg ataaagttac tgatgctctt
    11581 atgggtggac tagatagatt aaataatata tatataatgg cacattcagg agccagaggt
    11641 tctaaaaacc aaattagaca gctggcaggt atgcgtggtc ttatggcaaa tgcatctggt
    11701 aaaacagttg agataccagt taaatctaat ttccgtgaag gtttatcagt actagaatac
    11761 tttacatctt cacatggtgc cagaaaaggt cttgccgata cagctatacg tacagctgaa
    11821 tctggatatt taacaagaag acttgttgat gtaagtcaag atgttattgt aagggaaata
    11881 gattgtggaa cagaagatac tacagagatt tatgctataa aagaaggaaa tgaagttata
    11941 gaagagatat atgatagaat tgtaggaaga tatactatag accctatatt aaatcctgaa
    12001 actggagaag ttatagttga ggctgattca atgatacaag aagatgaagc agaaactata
    12061 gtagctttag gaattgaaaa aattaggata agaacagttc ttaactgtaa aactaatcat
    12121 ggagtttgtt ctaagtgtta tggtagaaac ttggcaacag gaaaagaagt taatataggt
    12181 gaagcagttg gtataatagc agctcaatct atcggtgagc cgggtactca gcttacaatg
    12241 cgtacattcc atactggagg agttgcagga gctgatataa ctcaaggtct accaagggtt
    12301 gaagaattat ttgaagctag aaaaccaaaa ggattagctg taataactga agtatctggt
    12361 agagttgaaa ttgatgaaac aggaaagaga aaagaagtaa atgtaatacc agaagaaggc
    12421 gagactcaaa catatgtaat tccatatggt tctagattaa aagttaagca aggtcaaatg
    12481 ctagaggctg gagaccctct aacacaagga tttataaatc ctcatgatat agtaagagta
    12541 aatggtgtta agggagttca agaatatata gttaaagaag ttcaaagagt gtatagactt
    12601 caaggggttg atgttaatga taagcatata gaagttatag taagacaaat gctatctaaa
    12661 gtaaaagttg aagacccagg agatacagat ttattgccag gtggatatga agatgtatta
    12721 acattcaatg aatgtaataa agatgctata gataaaggtc taagaccagc agttgctaaa
    12781 agagttttac ttggtataac taaagcatct cttgcaactg attcattctt atcagcagct
    12841 tctttccaag aaacaacaag agtattaaca gaggcagcaa taaaaggtaa agaagaccac
    12901 ttaataggac ttaaagaaaa tgttatatta ggtaagttaa taccagcagg aacaggaatg
    12961 aagaaatata gaaatatagc tgttgaaaaa attgaagatt aatagcatat agagtaaaga
    13021 aatcttgaca accattattt gtggtgttaa aatagtaaag tgtgttgttt aaaagtacac
    13081 taagggcttg aacaagttct tagtgacttt tagatggcaa taaatgagaa ctaaaaagca
    13141 tcaagctaga aaagtgctaa tatcacatat ttatgtgtta tagacattaa attgtaagta
    13201 acgaatgtta caatcagtca gtatgaatat tcatattgat gtagttggcg gtcactgata
    13261 agatcgaaaa tttggaagga ggtgcagatg atgccaacaa ttaaccaatt agttcgtaaa
    13321 agtagaaaag cgctagagaa gaaatctact gctccagcat tacaaaaagg ttacaactct
    13381 ttaaataaga aagtaactga cgctagtgcc ccacaaaaaa gaggggtttg tacttcagta
    13441 aaaacagtta ctcctaagaa gcctaactca gctttaagaa aagttgccag agttagatta
    13501 actaatggta tagaagtttc tgcttatata ccaggagaag gacacaactt acaagagcat
    13561 agtgttgttc ttataagagg aggaagagta aaagaccttc caggggttag ataccatata
    13621 ctaagaggta ctctagatac agcaggtgtt gataagagaa gacaatcaag atctaaatac
    13681 ggtgcgaaga gacctaaaga agctaaaaag taaaaacaat taatgattga ttatagttcg
    13741 gtgctttaaa tatatatata tttacggcat cacggcatta aatgatttat gtcgagtacc
    13801 tatgatttaa gttaaataat taaggaggga agcgaaatgc caagaaaagg taatattcca
    13861 aaaagagaag ttttaccaga tcctatgtat gggagtaagg tagttactaa attaataaat
    13921 aatctaatgg tagacggaaa aaaaggaaag tctcaaagaa tagtttatga tgctttcgca
    13981 attgttgctg aaaagacagg agaagaagct ttagaagtat ttaataaagc aatggataat
    14041 ataatgcctg ttttagaagt aaaagcaaga agagttggtg gggcaaacta ccaagtgcca
    14101 atcgaagtta gacctgaaag aagacaaaca ttaggtttaa gatggctagt aaaatatact
    14161 agagctcgtg gagaaaaagg aatggttgaa aagctagcta aagagataat ggacgcagct
    14221 aacaacactg gagcttcagt taagaaaaaa gaagatactc ataaaatggc agaagctaat
    14281 aaagcatttg ctcattacag atggtaggat atatagatat cttataaaat tgaggatatc
    14341 ataaggagga taaccatggc tagaaagttt cctttggaaa gaactaggaa tataggaatc
    14401 atggctcata tagatgcagg aaaaactact actacagaaa gaatcctgtt ttatactggg
    14461 caaactcata aaataggaga aactcatgaa ggagcttctc aaatggactg gatggagcaa
    14521 gaaaaggaaa gaggtataac aataacttct gccgctacta ctgcttcatg gaaagaccat
    14581 agaataaaca taatagatac tcctggacac gtggacttca cagttgaggt tgaaagatct
    14641 ctaagagttc ttgacggttc agttgctgta ttctgtgcaa aaggtggggt tgagcctcaa
    14701 tcagaaaatg tatggagaca agctgaaact tatggagtac ctagaatagc ttttgtaaat
    14761 aaaatggata tattaggtgc agatttctac aatgttgtaa gcatgatgaa atctagatta
    14821 aattctaatg cagttcctat gcaattacca ataggtaaag aagattcatt tataggtata
    14881 atagacttat taaaaatgga tgctgttata tacaaagacg acctaggggt tgaaatggaa
    14941 gaaacagata taccagaaga tatgaaagaa ttagctgctg agtggagaga aaaattagtt
    15001 gaatcagtag ctgaaactga tgaagaatta atgatgaaat accttgaagg tgaagaactt
    15061 actatagatg aattaaaagt tgctataaga aaagctacta tagcttgtga aatgaaccca
    15121 gttttctgtg gtacagctta tagaaacaaa ggtgttcagt tagtaataga tgcagtttta
    15181 gattacttac cagcgccaac tgatatacca gctataaaag gtatattagc agatggagaa
    15241 gaagctgaaa gacattcttc tgatgaagag ccattctctg cattagcatt caaaataatg
    15301 actgacccat tcgttggaaa actagcgttc ttcagagttt attctggaac attagaatca
    15361 ggttcttatg ttttaaatgc aactaagaat aagagagaaa gaattggtcg tatattacaa
    15421 atgcatgcta atacaagaga agaaataact aaagtgtatg ctggagatat agcagcagca
    15481 gtaggattaa aagatactac tactggagac acattatgtg accctgctaa tccaataata
    15541 cttgaatcta tggaattccc tgagccagtt atatctgttg ctatagaacc aagttctaaa
    15601 gcagctcaag aaaaaatggg tatagctctt caaaaattag ctgaagaaga cccaactttc
    15661 acagttaaga ctgaccaaga aacaggacaa actataatat ctggtatggg tgaattacac
    15721 ttagagataa tagtagatag attattaaga gaatttaaag tagaagctaa agttggtgca
    15781 ccacaagttg catatagaga gactataact caaccagttg atgttgaata caagtactct
    15841 aagcaatcag gtggtagagg acaatacggt cacgttaaga ttagagttgc tcctcaagag
    15901 ccaggtgaag gatacaaatt cacaaacaaa actgttggtg gatcagtacc aaaagaatat
    15961 gttggtccag ttgacatggg tatacaagga gctatgcaat ctggtatagt tgctggatat
    16021 ccagttgtag acgttgctgt tgagttatat gatggttctt accatgaggt tgactcttct
    16081 gagatggcgt ttaagatggc tggttcaatg gcattcaaag atgctatgaa gaaaggtaat
    16141 gcagttcttt tagagcctta cttcaaagta gaagttgtta ctccagaaga ttacatggga
    16201 gatgttatgg gtgacttaaa ctcaagaaga ggattaatcc aaggtatgga agctagaagt
    16261 ggtgcacaag ttataaatgc attcgttcca ctttcagaaa tgtttggata ctcaacagac
    16321 ctaagatctt caactcaagg tcgtgcaact tacacaatga tattcgatca ctatgagcaa
    16381 gttccagctt cagttgctaa gaaaatagct gaaggaaaat aatttaaaaa taattaataa
    16441 tataaatata aaaaataatc agctagatga gagatatctc tcatctagca caagat
//
LOCUS       DAYAOY010000094        16252 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYAOY010000094 DAYAOY010000000
VERSION     DAYAOY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.094"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            96..428
                     /locus_tag="ACGI6B_003068"
     CDS             96..428
                     /locus_tag="ACGI6B_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9752485.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(548..1189)
                     /gene="pcp"
                     /locus_tag="ACGI6B_003069"
     CDS             complement(548..1189)
                     /gene="pcp"
                     /locus_tag="ACGI6B_003069"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFG9752486.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(1210..2142)
                     /locus_tag="ACGI6B_003070"
     CDS             complement(1210..2142)
                     /locus_tag="ACGI6B_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFG9752487.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(2164..2829)
                     /locus_tag="ACGI6B_003071"
     CDS             complement(2164..2829)
                     /locus_tag="ACGI6B_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFG9752488.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(3401..3877)
                     /locus_tag="ACGI6B_003072"
     CDS             complement(3401..3877)
                     /locus_tag="ACGI6B_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9752489.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(3880..4284)
                     /locus_tag="ACGI6B_003073"
     CDS             complement(3880..4284)
                     /locus_tag="ACGI6B_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFG9752490.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(4259..4888)
                     /locus_tag="ACGI6B_003074"
     CDS             complement(4259..4888)
                     /locus_tag="ACGI6B_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9752491.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      5254..5297
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5398..6366
                     /locus_tag="ACGI6B_003075"
     CDS             5398..6366
                     /locus_tag="ACGI6B_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFG9752492.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            6368..6931
                     /locus_tag="ACGI6B_003076"
     CDS             6368..6931
                     /locus_tag="ACGI6B_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9752493.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            6939..7628
                     /locus_tag="ACGI6B_003077"
     CDS             6939..7628
                     /locus_tag="ACGI6B_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9752494.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            7755..8483
                     /locus_tag="ACGI6B_003078"
     CDS             7755..8483
                     /locus_tag="ACGI6B_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9752495.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            8497..9171
                     /locus_tag="ACGI6B_003079"
     CDS             8497..9171
                     /locus_tag="ACGI6B_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9752496.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            9197..10033
                     /locus_tag="ACGI6B_003080"
     CDS             9197..10033
                     /locus_tag="ACGI6B_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752497.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            10049..10720
                     /locus_tag="ACGI6B_003081"
     CDS             10049..10720
                     /locus_tag="ACGI6B_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752498.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            10710..11942
                     /locus_tag="ACGI6B_003082"
     CDS             10710..11942
                     /locus_tag="ACGI6B_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9752499.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            12092..12409
                     /gene="trxA"
                     /locus_tag="ACGI6B_003083"
     CDS             12092..12409
                     /gene="trxA"
                     /locus_tag="ACGI6B_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFG9752500.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            12426..13304
                     /locus_tag="ACGI6B_003084"
     CDS             12426..13304
                     /locus_tag="ACGI6B_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9752501.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            13574..13942
                     /locus_tag="ACGI6B_003085"
     CDS             13574..13942
                     /locus_tag="ACGI6B_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9752502.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            13945..14316
                     /locus_tag="ACGI6B_003086"
     CDS             13945..14316
                     /locus_tag="ACGI6B_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HFG9752503.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(14461..14916)
                     /locus_tag="ACGI6B_003087"
     CDS             complement(14461..14916)
                     /locus_tag="ACGI6B_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752504.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(14955..16220)
                     /locus_tag="ACGI6B_003088"
     CDS             complement(14955..16220)
                     /locus_tag="ACGI6B_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HFG9752505.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         6227 a         1910 c         2514 g         5601 t
ORIGIN      
        1 ttcaattatt cttacactat aattttatta taaattattc taaaaacaag tacgcacatt
       61 tttgttcaat actaaccaaa aggagagtgt aatatatgag tatagactgt atatcaaata
      121 cagatttaag tgaaacagga tttagttata cactttcact gattagtggt aaatataaga
      181 tgataattct ttactgtctt gtagaatttg aagttgtaag atataatgca ttaaaacgtt
      241 atataaatac tatctcctat aaaactttga gtttatcatt aaaagaatta gaagcagata
      301 acttgattat tcgtactgaa tatcctcaaa tacctcctaa ggtagaatat agtctatcag
      361 aaaggggaaa atctctgatt ccaattttag atgcaatgtg tgagtgggga gagaggaata
      421 gaccttaatt ttgtatctgt agataccata aaaagactgt gagtaaaatt gtttgaataa
      481 tctactcaca gtctatattt aaattaatag ttagtatttt taatttataa aaatactaaa
      541 aaatttatta tgaaatttca ccaccaattt cttttacatc ttctttatat ataatacaag
      601 cttctatagc taattctaag ccttttacta tttcttctaa agacatgaat ggtgtatttt
      661 ttttatctat tacttgtgaa gttgtatatg ggatgtgtat aaatccaccc cttatattag
      721 gatatttctt gtctatcata taaagaattc catacatcac atgattgcaa acaaatgttc
      781 ctgcagtgtt tgaaacacta gctggaattt tattttcatt catatgtttt accatagctt
      841 ttacaggcaa ttttgaaaaa tatgcaggtt caccatcttc ttttataata gtatctataa
      901 cctggttacc ctcattatct tttattctaa aatcatctat atttatagca actctctcag
      961 gtgtaatgtc aaatctacct ccagcttgtc caactgatat tactatatct ggattgtgct
     1021 cttgaatagc tttatctata gcttcaacag attttgtcat aactgttggt atagttatct
     1081 ttatgatttc tgcaccgttt atattattat tgaccctttc aactgcttct tgagctgggt
     1141 ttattggttc tccgccaaat gggtcaaatc ctgttaacaa aattttcatt attttacctc
     1201 ctaattatat taaaatccta aaaagtacat tactaatatg tgggctataa ttaataccaa
     1261 agcaactgga gcttgatatt ttataacaat ataatcattt ttacattcta gcaaggctgc
     1321 tggaactata ttaaaattag cagccattgg agtaagtaga gttccacaat atcctgctgt
     1381 taatgctaat gctccaatta tagcaggatt tccaccttga gacaatacaa aaggaatacc
     1441 aattcctgcc gttatgactg aaaatgcggc aaatgcattt cccattatca tggtaaatat
     1501 aaccatacct aatacataag ctataacacc aaataatctg ctatcagcag ggactacacc
     1561 agatatcatg gttgaaataa cttcaccgac tccggcttga gtaaataaag ctcccaatgc
     1621 aactaaaagt tgtggtaaca tactagcagg tcccatttgt tgaagtaatc ttgagccatc
     1681 ttgaactgat gtagcaggtt ttgccttagt tattataagg gctgatatta atgcaattat
     1741 tgaacccaca cctattgcaa actgagaaga attatcaaaa ttagaaagac tcatagctac
     1801 tactactgca gcaaatgcta atattaatga tggtataaat attttatttt taagtttgtc
     1861 tgcttgttct tttccaaaat tttcatctgg ttcttcatac tttgcaaata caacttgttt
     1921 agaaagtgtt aataaactag ataatattaa tattattcct attatattgg ctggaatcaa
     1981 tctaccaaat atgaatggca gtgatataag tatccaaaat aatgatgtac catatttctt
     2041 attgttattt atggtagtaa aagacttata tgctataaca atcattaaaa atcccattag
     2101 tgcgtaaaag atttccaatg atatgtttat aaaattttgc atttttattc ctcctaaata
     2161 aaactatttt ttatgacttt tgtttaattt tttatcatat aaggtaaact ggattgtagc
     2221 cactacaagt gctattactg ccataatagt actagccttt acgatattaa gtccagttac
     2281 attgtatcca aattgagtca gagtagatgc tataagtaat actccactac tacctgcaaa
     2341 taagttttga ccataaaaat ttccatagtt ttctgatgca gcagataatg cctttatggc
     2401 ttcttcatcc gattctttta gttcatcact attacttaaa ccagcagctt gtgccatagg
     2461 attaacaagt ggtcttacaa attgaggatg tccactcatt cttattgata gagcaccagc
     2521 tatttgtcta gctatcatgt atatagtaag tacttttcct gttgtaagtc tttttaattt
     2581 ttctattaaa gaaacagctc tttgtcttaa accatatctt tctaatacac cgattactgg
     2641 caatgttaat atgaataatg atacacctct gtttgagaca aaagaatctc ctaaaattgt
     2701 aaaaatttga ttaaaatcta atcctgcaac aattcctgtt gccactcctg ctaataatac
     2761 agtaaatagt gtgtcaattt taagtataaa tccaattaca actatcaata taccgattag
     2821 ttttatcata aattgttctc cttccatttc tatatgtttt aatttaaatt tatttaatgg
     2881 ataaagttaa actcttttta tagtatttgc gttaatagga ctattattat aaaatttaat
     2941 aaaaaatagg gaagatgttt taaagtatca aagtaaataa tggttgtgta taggaatttg
     3001 aataacaaag attatatttt aagctaatat gaattgattt tgtaagtcta tagtttaagt
     3061 atatgtcaat atttgaaata gtagttttaa attaaacatt atattataaa aagagaatgt
     3121 gccaaacata gagacaagtg tatttttctc tgttgaaatg ataaaaatat atttgatttc
     3181 atacaaaaag agagtgtcta atgaattttt tagttcatta gacactctct ttttgttatg
     3241 attaataaaa catatttgtt atgattaata aagcatatat taagtgctag tttcattaga
     3301 tttcactaag aaaaataaaa ttttgataaa gtttaaagac tattttgagc aatattactt
     3361 ataaataaaa gaatttatca tagaaataat tagctttatt ttagctatta agatttatta
     3421 aaaaattatc tatattatcc tgtgatattc taaaactgtc ataattacct tttgaaacag
     3481 ccttcataat aagtttatct aaaaatttca ttttttctaa tcgagcttca ctaccaaaaa
     3541 ttccaatagc atttgcactt tctaataagg atgatggaaa atttgatttt aaatatttat
     3601 ctgcttgttc agaaaagcca cagcaaagga aaatgccaac ttgttttttg tttaataact
     3661 ctacattatc attacatagc acctgtattt tttttgaaac tgaaccaaca tatatagaac
     3721 ttccaataat aattttatca aattttgata actcaatttt attattatta ttattaatat
     3781 caacaatagt gacattatct gatagtttat tttttagtat atttgcacag tcttttgtac
     3841 aaccatattt acttgaatat ataataatag tattcataat tattctccct ttcttaaagc
     3901 attttctaca gcttttcgta tagtattact gctataagtt gcagaagtta tagcatccac
     3961 atctaaagac tgttgtttaa taatacttgt tgtaattttt tcagcaggtt tacctaaaag
     4021 atggtcgtgt tttaagatat taatattttg gattttacca ttattaactt caacagacac
     4081 agtagctttt accaagttat tatcagcact tccagtataa atgccatttt taacattatt
     4141 aatatctata tcttttacta ccaagttttc tggctttaag ttgcttacaa ttacaataat
     4201 aatgcatata aatacaaaga atagagcaat agatattttt acatattttt tctttttatt
     4261 agtagccttg ttgcgaattt tcattgtaaa gtcctcccac taaaatatct aagaaatttt
     4321 ctataagttg agaaaaatta tgtaaagaat atagtttatt ttcaacaaca cataaaacga
     4381 atcctaacaa tgcacttata agcatccatg agcttttatc tatatttttt ttgaatattc
     4441 cttgttcaat tccaatggaa aggaatttat caagcatttt tataccatta ttgctaggcg
     4501 tattgtttgt attttcagat gcaaatataa tattcattct actatgcatg atagattttg
     4561 ccatctctgg ttcactagaa agagttttta taaagctagt cataatttct atgatttgtt
     4621 tatctattgg tagtgatgag caattattca taatgtcaat ggcattactt tctactgtgt
     4681 tataaagttt attggtcata tcgctgataa tctctgcttt gtctttgtag taaagataaa
     4741 ttgtagttgg agaatattct atttttgtag caatttttct tattgataaa ttttcatatc
     4801 catgttggtt tataatttcg atgctagcat ccataatctt tttcttcatt tcttcttttt
     4861 catttttacg tctttctttg atactcatat aatcacctct ttgcttaacg gtgttaagtt
     4921 aatttacact gttattgtat aatttaattt taatactgtc aataaattat taaataatat
     4981 aagttaatat atttttatgg ataataaatt aatgttatta attgtaatat ttaagatatt
     5041 attgtataat aataatcggt ttaaatgcgc cacatcttat taaagttggt tcataattct
     5101 aaataaactt taataagata cattaaaata taataaattc ttttatgcta taaaatttat
     5161 tttatagcta ttaaatcaga tgtttaaaaa ttttttaacg gtctatagtt tttaagagtc
     5221 ttatagtaag tagcctaaaa atatgatttt acatctgtac ttgttcgaga actccaagta
     5281 taaaaaacta aggaattata tagtggcagt atataattaa tacccttaga cacacctttt
     5341 tgaaagtgga aaaaagatgt gtagttctcc tcgaccacaa atcaaggagg ataaaaaatg
     5401 gaaaagagaa aagtaataat tgattgtgac ccaggaattg atgattcttt ggcaattctt
     5461 ctggctttaa actcaccaga gctagaagta attggaatta ccacatgttg tggaaatgtc
     5521 ccagcaaata taggtgcaga aaatgcacta aaaacacttc aaatgtgttc ttcactaaat
     5581 attccagtat atataggaga agaagcacca ctaaaaagaa aacttgtaac agctcaagat
     5641 acacatggag aagatggtat tggagaaaac ttttatcaaa aggttgtagg agctaaagca
     5701 aaaaatggag cagtggattt tataataaat actttatata atcatgaaaa agtatcaata
     5761 atagcacttg caccacttac aaatatagct aaagcactta ttaaagataa gaaagcattt
     5821 gaaaatctcg atgagtttgt atctatggga ggagcattta ggattcatgg aaattgctct
     5881 ccagtagcag agtttaatta ttgggtagac ccacatggag cagattatgt ttacaagaat
     5941 ttatctaaaa aaatccacat ggtaggttta gatgtaacta gaaaaattgt acttactcct
     6001 aatattattg agtttataaa tagacttgat aagaagatgg caaagtatat aactgaaata
     6061 actagatttt atattgattt ccattgggaa caggagggaa taattggctg tgtgataaat
     6121 gaccctctag cagtagcata ctttatagat agaagtatat gtaaaggatt tgaatcatat
     6181 gtagaagttg tagaagatgg aatagctatg ggtcagtcta tagtggattc tttcaatttc
     6241 tataaaaaaa atcctaatgc aattgttcta aatgaagttg atgagaagaa atttatgtac
     6301 atgtttttaa agaggctttt taaaggttat gaagacatta tagactctgt ggaaggagtg
     6361 atatagtgtg aaaagaaaaa tatgtgtaaa aactattaca cttatagctt ttggtatttc
     6421 tataaatata ataggcgctt ttatagctat gggattaaga ttacctgtat acttagattc
     6481 aataggaact ataatgatag cttcactttt aggacctaaa tacgctgttg ttacaggtgt
     6541 gtttggaagt ttaataagtg gaattacgtt tgatgtgtat tctctctatt ttgcaccagt
     6601 tcaaatatca accggattac ttgctggaat agcatttaaa aagaattttt taaggggttt
     6661 aaaaactcca ctaggagtac ttttatttgc cattccaaca tctataataa gttcaataat
     6721 ttcagcattt ttatttggag gtatgacctc ttcaggttca tcatatatag tgcaaatctt
     6781 gaaagtgtta ggactggggg atgtttttag tgtgtttgta acccaagtat ttactgatta
     6841 tggagataag ctgttagcag ttgttttagt gaatttagga ttaaatgcag ttccaaaaac
     6901 tttaaaggta tcattgactg gaggaaaata gatagaaaat ggatagatat agtgagataa
     6961 caaataaaaa tcaaagagaa attgttctgc ttaaaggatt tccttgtata tggggaaagt
     7021 gttctttttg tgattatata gatgataatt ctaatttaga agaagagatg aataaattaa
     7081 atctaaaggt tttaaaaaac gtaactggga aatatggagt tcttgaagtc ataaattctg
     7141 gaagctgttt tgaattacct aaggatactt tagaaaaaat aaagtgtatt ataaaagaga
     7201 agaatataaa aaaattattt ttagaaagtc attggtctta caaaaatagg cttaaggaaa
     7261 tgagagagta ttttgaaatt cctgtggtat ttaaaattgg agttgaaacc tttgacaatg
     7321 attttagaaa taatatttta aataaaaatg caaattttaa gactccacaa gatgtaaaag
     7381 aatattttga ttcaccttgt attatggttg gaataaaagg acaaacaaaa gagatgatag
     7441 ataaagatat agagattatc ctaaatactt ttgaaaaagc gacagtgaat gtttttataa
     7501 ataattcttc tagtataaaa agagatgaag agttagtgaa atggtttgtt agtaagtata
     7561 aattcttgga tgaaaatcca aatatagaag ttttatacaa taatacagac tttggtgtag
     7621 gagattagca ttaatttagt ttaaatttaa aattgaaaag tttcttatgt ttttgaaaaa
     7681 gcttctaata gttagaagct ttttttgtag agttttatgt tacaataaga aaaaaggaat
     7741 taaggagcaa ttaaatgatg tttgataggg gaaaatggaa taaattggat tataatgaac
     7801 ttttagaata tatgatgtct atacaagata taaaatatag agattttaat aaaaagctta
     7861 tacctggaac agaaaatata ataggtatta gagttcctaa tttaagaaag ttatcaaaag
     7921 aaataagcca agggaattgg aaggaatttt tagaagtagc acaggataca tactatgaag
     7981 aagttaggct acaaggtatg gtaattggca atataaatag taattttgaa gaaactttat
     8041 actatgtaaa aagatttata cctaaaatcg ataattggtc agtttgcgat ggattttgtt
     8101 ctgatttaaa gtctatgaaa aaatataaag aaaatatgta tgatatactt aaaaaatatg
     8161 tttattcaaa gaatccatgg gaaattagat ttgcattagt tatgttttta atatattatg
     8221 ttgatgataa acatattaaa gaaatatttg aatactgtaa taatatacaa agtgaagaat
     8281 attatgttaa aatgggtatg gcatggttgc tatcaatttg ttttattaaa tgtgaagagg
     8341 aaacttttct ctatataaaa aataataatt tagatgattt cacttataat aaaatgctac
     8401 aaaaaataat tgaatcaaat agagtagatt tagaaaagaa gaatataata agaagtatga
     8461 aaagaaagac aagaagatgt tgacggagga atattaatga ataacaatga agacagaatt
     8521 gacaaatttt tagaacttca aagcaaagga tttaaatttg cagagatagc tcaaaatcta
     8581 aagattagtc aaagtacttt aagaactttt ttaaataaaa aaggatataa gtcagaaaaa
     8641 ggtaaataca tattaaaaga cgaattaaat tataataaaa gtttggataa tagaaacttg
     8701 gaagaagaga aagctaagga gtatgaaaaa agtaaaaaga atactataag taatagaaaa
     8761 aaagtaagca ccaaaaaaaa gatttctaat gaaaaggctt taagtaaaag tgttgaacaa
     8821 attgagttta atgatacagg tttaaaaaat gcaaagccaa aacaaaatat taagcctaag
     8881 aaagatagaa aaataaatat aactcaagaa gatttagata agctttgtga agtatatgat
     8941 tggtatttag aagtaaaaga ttacaatata aagcctaaaa aagtatctaa taaaaaagat
     9001 gtacatatag atgatgaaca catagatgaa ttaaaaacaa caagaatacg tatagataaa
     9061 gaaatatgga atgattttga aagattatgt agtaattcga attttgaaaa acatcaaata
     9121 cttacacagg cattgaaaga atttatgaaa aagtataaaa atctactata aaatactact
     9181 atatgaggtg cttaatatga ataaaatagt aaaaaaatca atttctctaa tgattatatt
     9241 aacaatattt atttttatgt tgacagcatg tgaaaaagat gaacagcctg attcagttga
     9301 tattcaaaca gaagataaaa atgagataaa aatagatgag ggaaatgcaa aagtattgaa
     9361 tataggaaaa tcagaaatta taaatataga tgaaggtgat aaagtagata catcaacaaa
     9421 ggtagaaaat aattcaacat ttaatatatc caatgttgaa ttgatatata atgaatatga
     9481 tgagaataaa aaaattatat cttcggatag taaggctttg ttggacatga ctttaatgcc
     9541 tgggaaagtt gcttacatag aatgtgggca taaaactttt gtaaagggtg tagaagttta
     9601 tgcatatgaa tatgaggcag agggtaagat tgtgtatgtt aatttaaaag aaaatactat
     9661 taatataaga aataataata ttaagctaga aaatagtagt cagtatgagg tgctatctac
     9721 atcagaactt aaaaaagtaa atgaatcaaa tgagggaatt acgtatcaag taaaagtaaa
     9781 aaattcatca tcaaaagatt taggaaatat tattcttaag acagcagaag ttaatgaaaa
     9841 tggagaatat ttaacagtaa atcgtgtacc atcatataag attttaaagc cttcagaaga
     9901 gacagatata gatatattgt gttcaacaaa ggcaaaaagt gttgaaatag tgggatatac
     9961 gtatgatgat ataaaagaaa aagccaatgt ggatattgac ttaaaatctc ataaagttaa
    10021 gatagacaaa taaaggtagg ggatacttat gaaggtttta atagttgaag ataataagat
    10081 acttttagaa agtgtagtag aagaacttag caagcatttt gaaactgaaa agtgtgaaga
    10141 tggagaagaa gctttatact taataaatca aaatatatat gatttagtca ttttagactt
    10201 aatgcttcct aatataaatg gatttgatat cctaaagaaa atgagaatta ataatataga
    10261 tacacctgta cttatattga cagcaaaaga aacattagat gacaaagtag aagcatttac
    10321 tataggagct aatgattatt tgacaaaacc tttttatatg gaagagttag tagctagggt
    10381 atacgctata ttaaggacta atggaaagat aaaagaaaga aacggattag aatttaaatc
    10441 tttatattta gatacactag aaaaaagagt gtatattgaa aaagaagaaa taaaacttca
    10501 aaacaaacag tttaacttgc ttgagtattt tgtattaaat aaaggttcaa ttttattaaa
    10561 agagcagatt tatgatagaa tatggggaat tgattcagat gcaactattg aaatagtgga
    10621 agtatatgta agtaatctaa gaaaaaagct aagtaaatat ggatatgata aatatattaa
    10681 aactaaacgt aaggtaggat atatattcga tgataaataa gaatgtattt accagtacta
    10741 aaaatcattt aataaaaatg tatattattg tagtaggtag ttttttaata atcttttcta
    10801 tatttatata ctcatatttt aggggactta catatagtgg aattgatagt gagataaatg
    10861 atgaacttga atatatagtc tctcaattta aaagaacttc atttttgaac ccaataagac
    10921 tcaaagatcc taaagatatg gtgtatgtat atgaagatgg aaggataagt tactatactc
    10981 aaaatgaata ttttgatgag ttacttccag atagaagact agataaaaaa aattcgtttt
    11041 ttaaatacac agaaaatgga tatactttta gagaattaaa tgtagatgta ggtagatacc
    11101 aaatacagat aattagaaat atagattctg agatgaattc tttaagacag cttacatctg
    11161 ttcttataat tggaatatta atttctgtaa taattactta ctttgtggct gtatatctca
    11221 ctagaaaagc tttaattcca atagaaacag catggaaaaa tcaagctaag tttatacaag
    11281 atgcttctca tgaattaaga acaccaataa caatagtttc atcaaaatta gaaagtatgt
    11341 taaaatcccc agaaagtaca gtaaatgatg aagttgaaac aatagctact gcaatgaagg
    11401 aaactagaag acttaaaaaa atgataactg atttattgtc tcttactaaa gaggactcta
    11461 ttgttaaagt taatttagaa gaaatagatt tagaaaagtt attggaagaa atatcagaag
    11521 actatattga cattgctgag tttcaggaaa aaagattcgt gtttaattct aaactgaaga
    11581 ataaagttat aataacagat aaaaataaat taagacaact tatattaatt ttcattgata
    11641 atgcctttaa gtatacaaaa ttgggtgatg agatagcatt agaattgaaa gaagatatag
    11701 aggatgaggt aactttactt ataagtgata ctggtatagg cataaaaaaa gaagagatac
    11761 ctcttatatt tgatagattt tttagaagtg aaaatgtaag aaataaagat ttagaaggtt
    11821 ctggaatagg actttcaata gctcgcatga ttagtttaaa cttatctatt gatataaatg
    11881 taacttctga tgtagatatt ggaactacat ttgaattgtc aataccaaaa aagttaaaat
    11941 aataaaaata taaaaaattg tttttagaaa tgaagaatgg gtatactaat tagtataaaa
    12001 tataaaaaga ccttataaaa gtaaatttat atttataaat ttcatagtat aattatatag
    12061 ggataataaa gtttaatagg aggatttaaa aatggcaaaa gttataaata catctgaatt
    12121 tagaagtagt gttgaaggta gtaagggagt agttgtagtt gatttctttg caacttggtg
    12181 tggaccttgt aacatgttgg gacctgtatt tgcagaatta ggtgaagaga tgaaggataa
    12241 agctagattt gtaaaagttg atatagatga gagtttagaa atagctcaac aattcaatgt
    12301 atctacagta ccaactatga ttatatttaa agatggaaaa ccagtagaaa ctttaattgg
    12361 attcatgcca aagaacaaaa ttgaaatgca agtaaaatca tatttataaa aaatgggtga
    12421 gtattatgag atatgatata gctataatcg gcagtggtcc agctggattg tctgctgcta
    12481 taaatgcaaa aataagaaat aaaacaatta tcatgtttgg taatgataat ttaagtaata
    12541 agcttgttaa agctccttca atagataatt atttaggttt ttatgatata agtggtgatg
    12601 aattaaaaga caagtttaaa tcacatatag atagtatgga tatttcaatt gaaaataaaa
    12661 gaataaataa tatttatgct atgggagaat actttacaat aatgtctgga aatgatatgt
    12721 atgaagctac tactgtaatc cttgcaactg gggtagagta tactagacca atcaagggtg
    12781 aagaagaatt tctaggaaga ggtgtgggct actgtgctac atgtgatgca ccactttata
    12841 gaaataaaaa ggtagcagtt ataggatata atgaagaatc taaagaagaa gcaaattttt
    12901 taagtgaact cacttcaaag acttatttta tacctatgta caaaaaagat aatcttatga
    12961 gaagttcaga taacctagat gattctattg aagttataca tgacagacca gtacagatag
    13021 atggagataa acttgtaaac aaagtatctt ttaaagaaaa ccacattgaa gttgatggag
    13081 tattcgttat aaaagatagt acggctccaa gtgcattggt tcctggaata gagattgatg
    13141 gcattcatat taaagttgac aataatatga agactagtat agatggatgc tttgctgctg
    13201 gagattgtgt tggaaaaccc tattcttata taaaagcagc tggtcaaggg caaatagcag
    13261 ctttaaatgc tgtttactat ttagataaat taaagagggc ttaatattta tattgattaa
    13321 ctttttaaag atatttataa aaaactgtct tgaaataatt ttattatttc aagacagttt
    13381 tttattttta agttattaat aaaagtattt taaaatctat atgtaattac ttttattaat
    13441 aaaactcaaa gttatattag taaaaattgg aattatttat aatatactcc aaaatttaaa
    13501 aataatggtt gaaaaatcta attaagagta ctataattta attaagtaat tacttaatca
    13561 aaatgaggta cttatggaaa acttggttaa aatatttaaa gcattgagtg atgaaactag
    13621 acttaacata cttatattag tatccaagag aaatatatgt caaaagggga tttctaaata
    13681 tctagggatt tcagattctg ctgtttcaca acatataaaa atattaaaag atgttgggat
    13741 aataacagga tataaagaag gatactatgt tttatatcat attaataaag aatcatttaa
    13801 tatatgtgta gattttataa attctatgtt aagtaattct agtgaaagct ttatagatgt
    13861 atttgatgta aatacaatac atctaggatg cagtaaagaa tgtaaatcta taaaaaaatg
    13921 ttgtaagaga agggagaaat aataatgaaa gtatgtatac cagtagagga gaataaagga
    13981 ttggatagta aaccatatgg acattttggt tctgctccaa tttttgttgt gtgtgattta
    14041 gaaagtggag aagttaaatc tttagataat ggagatttag atcatgaaca tggaaaatgt
    14101 caaccattaa aagcattgtc agggacagct gtagatgcag tagttgttgg aggaatagga
    14161 caaggagcta taattaaatt aaatggaatg ggaataaaag tttacagagc agaaggtgat
    14221 actataagtg ctaatttaga cttacttaaa aatggaaaat tagtagaatt tccatcagac
    14281 catacttgct cacatgacgg atgtggacat cattaaaaaa aaacagctga tgaaatatat
    14341 acttcatcag ctattttttc ttttaataaa tacgatttta ttcttttaaa accaaaattt
    14401 agatttttat gttttttgta gtatccttaa taatatttca agtttacaag tttactactt
    14461 ttagattttt tttgtatgtt tttctatata gtcaatatca tttaatatgt cattgagttc
    14521 tttattaagt gtttttaaat catcaccatc tacattgtag tttaaaaata gttcctctac
    14581 attatcaatg atagaattga cttttctagt aacttcaaca aatgtcttaa agttactact
    14641 atttttagca tttagaaatt tttttaaatc ctttgatata aatgatacta tttcatattc
    14701 atcattaaat agacctctac caataggata tggttcaatt ctctgtaaaa aatattggtc
    14761 tttagcatta atattataag tataagatat ttcccaaccg tctcctaaat caaacttgat
    14821 aaaatataat gtactactta cttttgatat tcttgtggca ccaagctgtc ttaaaagaac
    14881 ttcaatcgaa tcaccttgaa cagtattaat attcatatat acacatcccc tttagtatta
    14941 ttatactata ttttttaaaa ttttataatt aaataaacag tagatataat aatagatata
    15001 atcataagtg gaaaacctat tttgaaatac tccttaaaag agataggatg accgtgttta
    15061 ttacctatac ctgctagtac aacattagca gatgctccta ttagtgttcc atttcctcca
    15121 agacaagctc caagagatgt tgcccaccat agtggcatta catcaatacc ttcagcttgc
    15181 atagtcaaaa taagaggaat tagtgttgca acaaatggaa tattatctaa gaaagaagag
    15241 actatagctg acaaccataa tatcaacaac attgtaaata caagatgtcc ttctgtaagt
    15301 ccaatcaatg cttgagccaa tttgtttatt atcccaactt ctactaatcc accaactact
    15361 acaaataatc ccataaagaa taaaattgta gaccattcaa tacctgccat aatttcatct
    15421 acatcttgtt ttcctattaa aagcattata gatgctcctg tcagtgctac aacagaagaa
    15481 tctatgtgga ttgtactgtg aaacataaaa ccaaaaagta tgaatgctat taatataagg
    15541 ctttttataa gtagaggttt atctttaaca gatttttttt catctagttt taatattgca
    15601 tttttagctc tttcattcac aacaagttct tttccataaa taaatctaaa gcatattata
    15661 gttatagcaa gtattactat aacagcagga cctaaattta ttacaaaatc cataaaacta
    15721 agattggcag cactaccaat cataatattt ggagggtctc ctataagagt agctgttcct
    15781 cctatatttg aagctaaaat ctgtgttatt aaaaatggaa ctggattaag tcctaatatt
    15841 tgtgtaataa ctatagtcat tggtccaata agtaagacgg ttgtaacatt atctaataca
    15901 gctgataaaa tagctgttat aatagcaaaa caaatcatta tcttccatgg gtcacctttg
    15961 gctttctttg cagtccagat agctatatat tcaaataacc ccgagttttt gacaacagct
    16021 acaaatagca tcataccaac taatacacca atagtattga aatctatatg gcttaatcct
    16081 tcatcaagtg taagtatatt gaatattatc attaatagtg caccaccaac agctgctacg
    16141 gatctgttta ccttttctgt aataatagct gccataacta ttaaaaatat acatatagct
    16201 attatttgtt gaaattccat aatataagta cctccatttg tattattttg tg
//
LOCUS       DAYAOY010000095        16223 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYAOY010000095 DAYAOY010000000
VERSION     DAYAOY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.095"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            251..1081
                     /locus_tag="ACGI6B_003089"
     CDS             251..1081
                     /locus_tag="ACGI6B_003089"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9752506.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
     gene            1491..1625
                     /locus_tag="ACGI6B_003090"
     CDS             1491..1625
                     /locus_tag="ACGI6B_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752507.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIETIRDIVDIIKILEPVYNFKSNQ
                     "
     gene            2130..2891
                     /locus_tag="ACGI6B_003091"
     CDS             2130..2891
                     /locus_tag="ACGI6B_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9752508.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2956..3738
                     /locus_tag="ACGI6B_003092"
     CDS             2956..3738
                     /locus_tag="ACGI6B_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFG9752509.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            4005..4424
                     /locus_tag="ACGI6B_003093"
     CDS             4005..4424
                     /locus_tag="ACGI6B_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HFG9752510.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4527..4793)
                     /locus_tag="ACGI6B_003094"
     CDS             complement(4527..4793)
                     /locus_tag="ACGI6B_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFG9752511.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            5020..5721
                     /locus_tag="ACGI6B_003095"
     CDS             5020..5721
                     /locus_tag="ACGI6B_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9752512.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5803..6411
                     /locus_tag="ACGI6B_003096"
     CDS             5803..6411
                     /locus_tag="ACGI6B_003096"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9752513.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            6735..7199
                     /locus_tag="ACGI6B_003097"
     CDS             6735..7199
                     /locus_tag="ACGI6B_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HFG9752514.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            7836..8852
                     /gene="gap"
                     /locus_tag="ACGI6B_003098"
     CDS             7836..8852
                     /gene="gap"
                     /locus_tag="ACGI6B_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFG9752515.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            9247..10416
                     /locus_tag="ACGI6B_003099"
     CDS             9247..10416
                     /locus_tag="ACGI6B_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752516.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            10796..15049
                     /locus_tag="ACGI6B_003100"
     CDS             10796..15049
                     /locus_tag="ACGI6B_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HFG9752517.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            15198..15692
                     /locus_tag="ACGI6B_003101"
     CDS             15198..15692
                     /locus_tag="ACGI6B_003101"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFG9752518.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            15808..16185
                     /locus_tag="ACGI6B_003102"
     CDS             15808..16185
                     /locus_tag="ACGI6B_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFG9752519.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         6592 a         1584 c         2862 g         5185 t
ORIGIN      
        1 aaaaatatga aaggatgatg ttgttcggtg atatttgata tcataaaatc gttgatataa
       61 gtattgctgg aaaagagtga ataaaatcaa atttaataag caaacaaagt aaagctatag
      121 ttttattgtt gtgcttaaaa atatttggtt ctattttact cttatttaaa ctcatctttt
      181 tgaagttaaa ccacagagta aactctgtgg tttttttatt ttaaaaataa attttaagga
      241 gtggtatgtt ttgttaaaca aattatgtat gtatttagaa agaccagagc tttataaaca
      301 aagtgaaatt aatttttggg atgatgagta tatttcagag caattattaa aagcacactt
      361 agacactaat tttgaaggag caagtagaaa ttttaacttt atcgaggatt ctgtaaattg
      421 gatagttaca gttgcaaatc cagttaatta tcctaaatta cttgatttag gttgtggtcc
      481 aggtttatat gcagagaaat ttgcacaaaa aggttataag gtaactggaa ttgatttctc
      541 aaaacgttct attaactatg cacaaaatag aaataaggag actaatttaa atataaatta
      601 cttgtttcaa agttatttaa atatgaacta taatgaggaa tttgatttgg ctaccttaat
      661 ttattgtgat tatggagctt tgtcaactga aaatagaagg ttattaatgg aaaaaattta
      721 tgatagttta aaacctggag gaaaattaat attagatgta tttacaataa ataaatacaa
      781 taattttgag gaaattaaat attgggaaat aaatgaagat ggaggatttt ggagtaatga
      841 aaaatatatg tgtctacaag ataattgtaa atacaatgat tataatacat tagaacaaac
      901 tcttgtaata accgaaaaag atgaaaatgt attttatgtt tggaatcact atttttcaaa
      961 agaaagtttc ttattagaag ttgaaaatat tggatttaag tctgttgagt tttttagtaa
     1021 tgttaagggt gaagcatatt ctgacgatag tatgactatg ggattagttt tacaaaaata
     1081 ataatttttt taaaatgaat aaataaaaac ttgaaacctt taacaataaa ataacataag
     1141 aaaagcttac atattgaaaa atggaaactt ttatgttatt tttagtacat tacaaatttg
     1201 tttatataaa aatcaatact tgaattatta tttttatgtg aaatttatct atagtatatt
     1261 tttataaaca agtaatgtta ttaaaattaa gtatatagtt aaaagtgtat agcaaaaagg
     1321 ctgttatatt aatgttagag gttcagggaa aatatataaa gcaaagatat ttacagataa
     1381 tgtagattgc acagctataa gttagataat agaattatgt aatcaagaat ttacaacaaa
     1441 gtataagata aggataatgc taaattgcca tacaggtgct agatgtgtaa atggatttaa
     1501 ttaatactat tgataaagtg caacaagcac ataaaccaat tgatgagatt attgaaacta
     1561 taagagatat agttgacata attaaaatat tggaacctgt ttataatttt aaatcaaatc
     1621 aataatgatt aagattaaaa taatattaag gcatcaatgt tataagagta caatataagg
     1681 agtactatat agtactcctt acttatacat attaggcaat agggaatttt tttatagtta
     1741 aattttcaaa tattttttag ggaactttat aattgagaat taagggtata gtatgtgatg
     1801 aacttactaa atttataatt aggttatata atggaataat catcaatagt tttactttag
     1861 ggaaaatata tgcttaagga agtatagctt actaatatta ttaaaatgta tatttaaaat
     1921 gataaaaaaa gaattagaca agtatttagt aaattgacta tgcaaaattc tatcaagcag
     1981 gaacactatg actttagtca tgggaggttc agtaggtaac ttgttgaatt aagtttatat
     2041 tatttaaata aactacttaa tttagataat ataaaaagtc agaattttaa gaatagagct
     2101 atataatctt agctattagg agaagtacta tgataggaat atattttagt ggaacaggaa
     2161 attcaagata ctgtgtagaa aaatttttac aagaatatga tataacagca aattcttatt
     2221 ctattgaaga taatgaactt ttacagcaca tcaataatca tgagaatatt atttttagtt
     2281 atcctgtgca atatagtaat gtgccaaaaa tattgaaaga ttttataata aatcattcaa
     2341 ctttgtggaa tggtaaacgt gtttttatta ttgcaactat ggctctattt agtggagatg
     2401 gctctggtgt gttagctaga atattaaaaa aatacggtgc tataactgtt ggtggattac
     2461 atgtgaagat gccagatagt ataggagatg aaaaggtatt aaaacattct ttagagtata
     2521 atagaaagct tgtaataaaa gcagaaaaga aagtaactaa agctgctaaa aaactgaaac
     2581 atgggtcacc accaaaagaa gggttgggtt ttttgagtca tattgtaggg ttatttggac
     2641 aacgattgta ttttatcaat aaaacaaata tgtatacaga taaactaaaa attaatactg
     2701 aggaatgtat tgtatgtggt aaatgcgtta atttatgtcc aatgaagaat ctagtaatta
     2761 aaaattgtat gattgttgca aatgggcaat gtacaatgtg ttatcgttgt gtaaataatt
     2821 gtgccaaaaa agcaattaca ttgttaggta aaaaagttgt tgaacagagt aatattaaaa
     2881 aatatatgta agaaatttaa agttttattg aatgtaaatt gatgtatatt ttatgttaga
     2941 aagagaggtt aaataatgga atactacata gttgattcat ttgcaactaa actatttaaa
     3001 ggaaatcctg ctggagtttg tgttcttgac agaagaatac ctttagagtt aatgcagaaa
     3061 atagcagaag aaaacaattt accagaaaca gcatttgtag ttaaatgtaa aggaaattat
     3121 gaattacgat ggtttactcc taaagcagaa attgatttat gtggtcatgc aactttagca
     3181 gcagcttatg taatatcaaa ttttattgat gttaatgtaa aaaggataga tttctttact
     3241 caaagtggaa agcttgaagt tacacgaaat ggaaatctat atgaaatgat atttccagaa
     3301 ataatgccta ttgaaataga gttatcacca caacaagcta atttgatagg ctgtgtacca
     3361 agtgctgttt attcttcaag agatttaatc ttattgctaa acagtgaaca agaagttata
     3421 aattataaac ctaattacgc acaattgcgt aaattgacag actggttagg tataataatc
     3481 acagcacaag gaagtaatac agattttgta tcaagatatt tttgtccaga attagattca
     3541 gaagaccctg ttactggttc atcacattgt aatcttattc catactggtc tgaaaagttg
     3601 ggaaaacaca aaatggtagc tgctcaactt tctaatcgtg gtggtataat tcagtgtgaa
     3661 gtgttgaagg ataatactgt aaaaatttct ggtgaagcag tcttgtttat gcagggaaca
     3721 ataaagattg atatttaaat aagtataata actaatttat tatgaaaaat aattaatttt
     3781 cttagtgtta acaataataa agtagagtta aaaagcttga tttcacttaa atcaatatga
     3841 tagatgttga tactaagatt gacaagcatg attaaaaaag ataatattta atctataaag
     3901 ataaaaacac agtctggttg gtagtccaga cgcagcaata gctgtcagta acctgcctcc
     3961 ttggttgtcc gttctttaga aagtaattta aggaggaact agcaatggac aaaattagtg
     4021 gaaaattaac agttttattt gaagaaccat tttgggtagg tatatttgaa agacaagatg
     4081 gtaaaaaata tgaagcatgt agggtagttt ttggagcaga acctaaagaa gtagaagtgt
     4141 atgaatttat acttgaaaga tttttctcat tagattttgg tagcattaaa ttagaaaaaa
     4201 atgtgaccaa agacaacatt ggatacaaaa gaatgcagag aaaagtaaaa aaggaacaag
     4261 aaaaagaaac tatagggact aaggctcaaa atgctcttaa gcttcaatat gaagaaagaa
     4321 aacaggatag aaaaaaccta gctaaaaata gaaaagaaga agaaaaagaa agactattta
     4381 atttgaaaca agaaaagaga aaagctaaac ataagggtca ttaattttaa ttaaatatct
     4441 ttttaataat tgctagaaaa aggtgtataa aatttttaat tttatacacc ttaatttatt
     4501 gaaaagaata aggttttata tttcaattac tttaaaatat aaaaatatac taataaaaat
     4561 tgtaaaaata gtataaatgg tataccaaat gtaaatttaa tgtgtttagt tttatgtcta
     4621 aaagaataca ttccaattat agaacctata ctacctccaa ttactgctat agacataaga
     4681 gtagcttctt ttattctcca ttcgtttcta attgctcttt tcttatctat gtacattgaa
     4741 aagaaagcaa tagaattaat cactataaaa tatattagaa taaaattttt cacagtcttg
     4801 cctccataaa aatatattat acattataaa acaagatatt tgttttggaa agttaaattt
     4861 gtaattagta catataaaat atttgataag aacaaatata atattaattg gaaaatttaa
     4921 aaaaattaaa caattagcaa atagggggta tatttataag acacaaagtg ataaaatcta
     4981 tttagaaaat aatgtaaaat aacaggagga taaagcaaaa tgaaatttat agtagaaaaa
     5041 gaagtttttg ataaattaga aaatgtatgc tttggtgtag tagttgcaaa aggaatagat
     5101 aatactaaag aaatagaaag aataagcaac cttttagata taagtataga tagagtagaa
     5161 gattacttca aagataaaaa agtaaaggaa tcagaagaaa ttataccata tagagaggca
     5221 tttagaagtc taggcatgaa cccaaataaa tttatgagtt cgatagaagc aatgacaaca
     5281 agggttgcta agaataagaa acttcctcat ataaatccta ttgtagattt aggaaactct
     5341 atttctttaa aatatttact tccaatgggt gctcatgata tggattttag aaatgatgat
     5401 gtatatgtta gattttctaa aaaaggggat aaatttgtac catttggaga aactgatgtt
     5461 gaattaatgg aagaaggaga attaatttat tcagttggag acatggtaaa aactagaaga
     5521 tggatatgga gacaaggaga ggaaggaaaa attactaata gctcaaaaaa tatatttttt
     5581 cctatagatg gttttactga tgctaattta gataaggtaa tgagtgcaag agaagaatta
     5641 gcaaaattgt taaaggaaat atttaattgt gaaattaaag taggttttgt agataaagat
     5701 aatcctgaaa tggaaatata ataagttgtt gaatacttgt taagatatta tatttactta
     5761 atgatgttaa aaaggtatta aaaaagaaag gtcgtagtaa atttgaaaaa aactataata
     5821 aagaatgtaa atccaatgat tgaaaataag tttataggac tttttgaaat agagtataaa
     5881 aacaaattag atgaagataa ggtttggatg gtagcatcaa gaaaaagtag tgagcaatta
     5941 aaaaatattt atttgaagaa tgaagaagat agtgtagatg cagttgtaat agttgggctt
     6001 cataaaagta gtaaaaaatt aattttaata agacaattta gagtacctat taatggatat
     6061 atatacgagt taccagcagg tcttgttgat gaaggagagt ctatagatat ttccgttgaa
     6121 agagaactta gagaagaaac aggacttact cttttagaaa ttaaaaaaaa taaaagtatt
     6181 gataaagttt atttatctcc tggaatgagt gatgaatctg ttgcctttgt ttattgtata
     6241 tgtgatggtg atataacaga tgaattttta gaaccagatg aagagattga agcgctttta
     6301 atttcgcaag aagatgcaaa agagatttta caaagtaatc ataagataga cacaaaagca
     6361 tttttgattt tacaaatgtt tgtgagttta ggagctaaat tatttgaata gatatataga
     6421 aaagaggtta tatagaagag taaaataata aagtttattt tacttgtata aattataatc
     6481 tcttttttta tacaataaag tataattttg tgtattttta aataagtgac ttgactctta
     6541 aattttcata atatgttata aaccaattta agatactaca ataattaaaa ttccaaaatt
     6601 atttaacata tttgcaatta aaatgagaaa aggtaaaggt tgaatgattg aaaaatttga
     6661 tgtaaaatag tagcttagtt taatattata gtttgtaggg ttaaaaatta gaaattgtaa
     6721 gggagataaa ttctatgaaa aaattagtta ttattttaat tagtatcatt agtatggtta
     6781 gcttgacagc ttgtaaaaaa agcaatgaaa gtaaatataa taatttaaac aacaaagaat
     6841 ccggacaaac aaccattgaa atggacttag ataaaaatta tgatacttca gacccatttg
     6901 ttaatgcacg tttgttttgt gtgtctaatg atatagatgt tttagacaca gagatttctt
     6961 ttaaaatgga tggagatagt ggtattgtgg aaataaagga caataaaaca tatgaaactt
     7021 tatggagtaa tacatggcat ggaagcgttg ataatgatac attcacaatt tctctttcta
     7081 atatacaaaa agagaaagag tatgctatat gctttactgg aattaaaata aatcatgcag
     7141 ttgtaaaggt tacttttgaa agcaatttag ttaaggagaa agaaaaaccc tcaaaataaa
     7201 ttccagattg tctataactt gatattcagt acataaaagc agttgatatt tatgattgac
     7261 tgctttttct tatcaaaaag gagagtttgt gcatttttac ttgaaatagt ttatgcacat
     7321 caaaagttgg tgagcatgat attggaaatg gtaagcatga aatcttgata agacaaggaa
     7381 ataaatttaa gttttcaatt aagtatattt ccataattta gttaacaata agctaattat
     7441 tatttatatt ttgatatata aataataatt gtagcaaatt tcaaaaaatc atatattttt
     7501 tgaactaaat aaaaatttat ttttattatt ttaaattaga ggagattctt gaagatatat
     7561 tatttttaaa aataagtata gtttaaaaat tcaaaaaaaa gatggcaaaa tatgtcccaa
     7621 gggacattga gtaaaataaa tgatttcagc ttatttacac taaaaagtac atactaaatg
     7681 aggggtaaaa taaagaaaaa tgatatacta attgtctttt gataataaaa aaacatggaa
     7741 tttgattatt taatcaagaa aaagtattga acaaatgatt tatttagtgt acaataatca
     7801 tgtaaaaaca tagtaatgga gggagacatt tcataatgaa aattaaggta ggaatgaacg
     7861 gatttggaag aataggaaga gctgtattaa gaatagctca agaagaatta ggagataata
     7921 tagaaattgt agccataaat gcaagagcaa caacagaatc tttggcacat ttatttacat
     7981 atgattcatg ctatggaact tttagaggtg aagtagaggc aaaggatgaa gacactctag
     8041 tagtaaactc taaagagata aaaatattaa gatataatga cccagaagaa ttaccatgga
     8101 aagaactggg ggttgatata gtaatagaat caacaggatt atttacacaa agagaaaagg
     8161 cagaaaagca tattaaagca ggagcaaaaa aagtaattat aacagctcca ggaaaaaatg
     8221 aagatataac tatagttatc ggagttaatg agaaagaata tgataatgaa aagcacaata
     8281 taatttcaaa tgcatcttgt actacaaatt gtctagctcc ttttgctaaa gtattggacg
     8341 aaaaatttgg tatagtaaaa gggttaatga ctacagtaca ttcatatact aacgatcaaa
     8401 gaatattaga taaaagccat aaagatttga gaagagcaag agcagcggct gaatctataa
     8461 tacctaccac tacaggagca gcaaaagctg tgtcaagagt actaccacaa ttagaaggaa
     8521 aattaaatgg tttttcactt agagtaccaa caccaacagt ttcacttgtt gatttagtgt
     8581 gcgaacttaa agaaaacgtt acatctgagc aagttaactc tgtgttaaaa gatgctgctg
     8641 aaggagaatt aaaaggtgtt ttaggatatt gtgacaagcc acttgtttct atagattata
     8701 aaggagattc aagatcatct attatagacg ctctatccac aatggttata gaagacaata
     8761 tggttaaggt agtttcttgg tatgataatg aatggggata ttcttcaaga actgttgact
     8821 tagtaaaata tgtagctgaa aaattaaaat aatatatatt tcttaaagca aattttaaaa
     8881 aaatttttta aaaaaattat ataaaactaa aaaaaaagat tatgaaaaat attgaaggaa
     8941 aaattttttc ataatctttt ttctttcatg caaaaagata caagttacaa cttatatttg
     9001 catattaggt cgttagagaa gtatatatat atttagtaag gaatcatact ttttataaaa
     9061 tgaaagataa gatgtcgaaa acttgcataa atctgaaaaa tttttattat taaaatactg
     9121 atttccattt taaaaatgat aaaaaaatga caaataaagt tgcaaatagt aatacttata
     9181 agtataatta aaaaagagaa aaggttttgt taaactttat aacaaaaaaa gaagggatga
     9241 ttcaaaatgg agaagttaac agacaggaga caggttaagg cagtaagcat ggaaaccttc
     9301 attttcctaa tattactagt agttggtttt gggtatgttg gtagtattat gggagcagga
     9361 atgatgttca aagtaataat gagtacagca catgctttac tattggagac agttttctta
     9421 attatggcta tggcagttct tgcaggcgca ttaagcgcac tactttcaga gtttggagta
     9481 atagcgttaa ttaataaaat tttcgctgta tttatgaagc ctttatatgg actacctggt
     9541 gctagtatag caggagctat aacaacttat ttatcagata atccagctat tatacctttt
     9601 gctaaagata aaacattcac acagtatttt aaacaatacc aagttccagc attatgtaac
     9661 ttaggaacag cctttggtat gggacttatc ttaacaacat ttatgatttc tcaaggtaca
     9721 gaatatgtac ttccagcatt aattggtaat ttaggtgcaa ttataggtag tgtcataagt
     9781 gttagaataa tgttgacatt tactaagaag ttttataaat acagtccgga agaagataaa
     9841 gcaacaggaa cattagagaa aaaggatgaa tttagagaaa tcagagaagg aaatgtattt
     9901 caaagagctt tagatgcaat tcttgaaggt ggtaaaatgg gggtagatat gggaatggct
     9961 ataattcctg gagtcctagt agtatgtaca ttggtgatgt tattaacttt tggaccatct
    10021 acagaccctg ttacaggtca agaagtttat acaggagctg catatgaagg aataaaatta
    10081 cttccagtta ttggagataa acttggattt atattagaac cattatttgg atttacatca
    10141 ccagaggcaa tagcattccc tataacttca ttgggagctg ttggcgcagc aatgtctcta
    10201 gttccagagt ttataaagtc aggagctata actcctaatg atatagcagt atttacagct
    10261 atgggtatgt gttggagtgg atatttaagt acacatattg gtatgatgga tgcattaaat
    10321 gctagacagt tggcaggtaa agctattctt tctcatacaa taggtggttt atgtgcagga
    10381 gcagctgctc actttatatt tacactcgta ggataaatgt aatcagctaa gataaaaaat
    10441 aatatacaaa aaatggttgt tgatatgtaa taagaattta catatcaaca accatttttt
    10501 tctgactaaa gataatttat aatacataaa cattaagtgg atattagagt taagttatat
    10561 ttatgtaaaa agtaacaaaa atctgtagaa atttatgaca aaatttagta cactaactat
    10621 atgttcttat ttaaaattta aggttagaat taggaattac ctagtagagt aagctttgat
    10681 taataaaaga atcataaaat aaaaatatta gtcataaaga ttataaataa cttacttaaa
    10741 ataaagccat agaacttaca aaaattaaaa aagcatataa atatggaggg gtacaatgga
    10801 tgaattattt catataggaa ttgatgtagg ttctacaacg gtgaaggttg tggtacttaa
    10861 taatagtaat aatatagttc ataaagaata tagaagacat tattctgatg taaaaaaatc
    10921 agttaaagaa gtactaaatg gtatatatga aaaattggga gatataaata ctaccattat
    10981 aataactggt tcaggtggaa ttggaatatc aaaaaaatta ggtgtaaaat ttgttcagga
    11041 agttatatct agtacaaaat caatagagta ttttcatcct gagactgatg ttgtcataga
    11101 acttgggggt gaagatgcaa agataacata tctaagtgga ggaatagacc aaagaatgaa
    11161 tggaatatgt gcaggtggca ctggagcatt catagaccag atggcatcac tcttaaagac
    11221 agatgccagt ggattaaatg aacttgctaa agggtataat gttatatatc ccatagcttc
    11281 aagatgtggt gtttttgcta aaacagatat tcaacctctt ataaatgatg gagccaatca
    11341 gactgatata gcaatgagta ttttcaatgc agttgtcgta caaactgtga gtgtcctttc
    11401 ttgtggaaga aaaatagaag gaaatgttgc gtttttgggt ggtccattgt actttttatc
    11461 tgaacttaga gaagcattta aaagagtatt agatttaaaa gatgaaaaca taatcttccc
    11521 agaaaatgca caactttata ttgctattgg agcaggatta ttatcagtag atgaagataa
    11581 tatacaacta aaatctctaa tagaaaaatt ggatagtata aagaatattg aagatggaga
    11641 agtaaattta ttagaacctt tatttaaaga taaaaatgag tatgaagaat tttcaagaag
    11701 acatgaaaaa gaaaaaataa attatgtgga cataaattca ataaaaagca attgttattt
    11761 aggaattgat gcaggctcaa ctacaactaa agcagcactt attgatgagg atggaagact
    11821 tgtatattct tattataata gcaatgaagg aaatccatta aaaacaacca ttaaagttat
    11881 aaatgaagta tatgatatat taccaaaaaa tattaaaata ttgagttcaa ctgtaactgg
    11941 atatggagaa ggtcttatta aaaaagctct taaaatcgat aatggagaga tagagactat
    12001 agcacattac aaagctgcta aatttttcaa caaagatgta gattttatac ttgatattgg
    12061 tggtcaagat atgaagtgcc taaaaataaa agatggagta attgataata taatattaaa
    12121 tgaggcttgt tcatcaggtt gtggctcatt tttagagaca tttgcaagtt ctttatctat
    12181 gacaatagaa gaatttgcgc atgaaggtat ttattctcaa aatcctgtag atttaggttc
    12241 aagatgtact gtatttatga attctagagt aaaacaagct caaaaagaag gtgcaagtgt
    12301 tggagatata tcagcagggc tatcttattc agtaataaaa aatgctttat ttaaagtaat
    12361 aaagattaga gatttaaatg agattggtaa taatattgtt gtacaaggtg gaacctttta
    12421 taatgatttg gtgcttagaa gctttgaaaa actaattgga aaaaatgtaa ttagacctaa
    12481 tatttcagga attatgggag cttttggttc agcacttata gctaaagaaa aatatgaaag
    12541 tggatacaaa acatccttgt tatctaagga agagttgaat aatatcaagc tagaagcaag
    12601 tgtaactaga tgtaaaggtt gttcaaatca ttgtcttctt acaataaata aattttctga
    12661 taatgaaatt tttatttcag gtaatagatg tgagaagggt gaagctattt atggtgataa
    12721 aaaaatagga ttagaaaaga aaaaaccaat aaatttattt aagtacaaat acaatagaat
    12781 ttttagatac aaaccattgg aaaaagaaga agcaaaaaat ggggaaatag ggataccaag
    12841 agtattaaat atgtatgaag attatccatt ttggtttact tttttcaatg ctatgggctt
    12901 tagagttgta ctttcagata ggtcatcaaa acagttatat gaaactggta taactagtat
    12961 tgcatctgaa acagtttgtt atccaggaaa attagttcat ggacatatag aaaatttaat
    13021 tgcaaaaggt ataaaaaata tattttatcc aagtgtaaca aatgaaaata aagaggattc
    13081 aaatgcagat aattattata attgtcctgt tgtaatatca tattctgaag taataaaaaa
    13141 caatgtagag aatataagaa ataagaacat aaattatata aatccattta taagtctaaa
    13201 tgataaggaa aaattgaaga aaagactcta tgatgaatta tcaagacatt tttctgatat
    13261 aaaaataact aaagaagaag ttaatcatgc agttgataaa gctactgatg aacagaattt
    13321 atttaaaatg gagatacaag cagcaggtga aagagcactt aaagaaataa aagaaagaaa
    13381 tatgaagggg attgtgttat gtggaagacc atatcatata gacccagaaa taaatcatgg
    13441 aatgccagag cttataaatt cattagatat ggcagtgctt actgaagact ctatatgtca
    13501 tttagccaat gtacaaagac cacttagagt tgtagaccaa tgggtatatc attcaagatt
    13561 gtataaagca gctagttttg taagagataa aaactatttg gaacttgttc aacttaattc
    13621 atttggctgt ggattagatg cagtaactac agaccaggtt caagaaatac ttaatgaaaa
    13681 atcgaagata tatactatta taaagattga cgaaggcaac aatttaggag cagctaagat
    13741 aaggatgagg tccttaaaag cagctatgtt tgagagagaa agcaaaaata tagatgtaaa
    13801 aaatataaag gtaaacagga tacagtatgc taaaaataat cagataacga acaaacatac
    13861 tatattagca ccacaaatgt ctccaataca tttccaattc cttgaaaaag ctgttaactt
    13921 aagtggttat aatatagaaa ttctaaaaga tactgatagt agtgtaattg aagaagggtt
    13981 aagatatgta aataatgatg catgttatcc agctataata gttgtaggtc agctaataac
    14041 agcattaaaa tctggcaaat atgatttaaa taacacttct gttactataa ctcagactgg
    14101 tggaggttgt agagctacaa attatattgg attcttaaga aaggctatat acgatgctgg
    14161 atttaaagat gttcctgtca ttgcacttag tgtaaatgga atagaagata gtggtataat
    14221 ggataatata tcactaaaat taatcaatag actctttatg tctgttgttt atggtgattt
    14281 gctgatgaaa gtactatata gagttagacc atatgaaaaa gttactggaa gtgcaaatgc
    14341 tctatatgaa aaatgggttg atatatgcaa atcttctctt gttaaagcaa aaatttctgt
    14401 atttactaga aatataaaag atatagtaaa agaatttgat aatttagaga tactagatat
    14461 caaaaaacca aaagtaggtt tggttggaga aatattggtg aaattccatc caattgcaaa
    14521 taataattta gtagatatat tagagcgaga aggtgctgaa gcagtcgtgc cagaccttac
    14581 aaatttcttc ttaagttgtg cctttaatac tatatacaaa cacactcatt tagaaggaag
    14641 tagaaagagt agaatgatag gagaggcatt tatatacata actggaatat atcaaagagt
    14701 atataaaaaa gcacttgata agagtgagag attttatgct ccagccaata taaaaaatgt
    14761 tgctaaaagt acagaaccag tagtgtcatt aggtaatcaa actggagaag gttggctttt
    14821 aacaggtgaa atggttgaat tattaaatga aggtgtagaa aacattattt gtatgcaacc
    14881 ttttggatgt ttacctaatc acataattgg taagggttct ataaaagaat taaagaggtt
    14941 gtataaaaat gcaaatataa tacctattga ttatgaccct tctgcaagtg aggtaaatca
    15001 acttaacagg ataaaactta tgctttctaa ggcttttaaa aatatataag atttatattt
    15061 tgatatgtta aaagttaatg aatttaaaaa tatactctat attaagtatc tataaaaaag
    15121 agttatccta ttttttaggg taactcttta ttagttaaaa aattatatgt atactgatat
    15181 taaagggggg aagtaatatg agtttagtat atgatgttta taaatcaaaa ttaggaaatt
    15241 tgtacatatt aagtgaagat ggttttatag ttagtatata cataggagat gaaaaattta
    15301 ataatttaaa aaatgaatgt attaaaaaag gaattgatat aaagagaagt aaggaagatt
    15361 taaaggaagc aataagccag atagatgatt actttaatgg aaaaagaaaa gtctttaatt
    15421 taaatattcg tattgaaggg actgaatttc aaaaatcagt ttatagggaa atgttaaaaa
    15481 taccttatgg agaaacattg tgttattcag atatagcaag aaatataaac aatccaaaat
    15541 cagtaagagc aataggacaa gcaagtaaaa gtaataaaat tccaataata ataccttgtc
    15601 atagagtagt tggcaaaaaa gatattggtg gatatatggg gaatcattct gatttgaaag
    15661 agatactatt aaatttagaa agacagagtt aacttagaaa gaaatgtttt tattgttaat
    15721 atttgatata ataatagtaa aaagaagtat tttttacaaa aacaatgaag aatttatttt
    15781 aatttacaaa aatgaggggg aattttaatg tgtgataaag ttatagcatc aaaatatcat
    15841 gagaagggat ttaattgtgc agaatcagtt ataaaagcat ataatgagga atttaataca
    15901 gatataccag tttgtttagg aagtggactg ggttcagggt gtggggttgc tagtttatgt
    15961 ggtgcagtaa atgcatcaaa tataataatt ggatatgtaa aaggcagaaa ccacgaagat
    16021 gaaagtacta aagctaaagt atatgctaaa gatttaacta caacagtaag aaaagagtat
    16081 ggttcagagt tatgtataga cctaaaaaaa gatttagttg cttgtagaga aataatggat
    16141 ttcgcatatg atagtctaaa ggaaacttta aaaaaagaat tataagaaaa taagctatca
    16201 aatataaatt atttgatagc tta
//
LOCUS       DAYAOY010000096        16076 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYAOY010000096 DAYAOY010000000
VERSION     DAYAOY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.096"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            513..2747
                     /locus_tag="ACGI6B_003103"
     CDS             513..2747
                     /locus_tag="ACGI6B_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9752520.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2778..3623
                     /gene="dccA"
                     /locus_tag="ACGI6B_003104"
     CDS             2778..3623
                     /gene="dccA"
                     /locus_tag="ACGI6B_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HFG9752521.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3863..5596
                     /locus_tag="ACGI6B_003105"
     CDS             3863..5596
                     /locus_tag="ACGI6B_003105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9752522.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5922..6408
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6054..6082
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6938..7042)
                     /locus_tag="ACGI6B_003106"
     CDS             complement(6938..7042)
                     /locus_tag="ACGI6B_003106"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFG9752523.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7667..7912
                     /locus_tag="ACGI6B_003107"
     CDS             7667..7912
                     /locus_tag="ACGI6B_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752524.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8409..8543)
                     /locus_tag="ACGI6B_003108"
     CDS             complement(8409..8543)
                     /locus_tag="ACGI6B_003108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752525.1"
                     /translation="MLDKRLGKRRLKNIYEDIEKENNLVKKFYNIRVESENMILKKNA
                     "
     gene            complement(8665..8811)
                     /locus_tag="ACGI6B_003109"
     CDS             complement(8665..8811)
                     /locus_tag="ACGI6B_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752526.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8907..9230
                     /locus_tag="ACGI6B_003110"
     CDS             8907..9230
                     /locus_tag="ACGI6B_003110"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFG9752527.1"
                     /translation="MKDRECSFSPNTIRNRKNMIKNQIEVFFKDMKLIDITPSILQSY
                     INNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENICDFVTTPIKKVVQLLSFIQNK
                     KLNYY"
     gene            10097..11452
                     /locus_tag="ACGI6B_003111"
     CDS             10097..11452
                     /locus_tag="ACGI6B_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9752528.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11570..12229)
                     /locus_tag="ACGI6B_003112"
     CDS             complement(11570..12229)
                     /locus_tag="ACGI6B_003112"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HFG9752529.1"
                     /translation="MLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTISEEH
                     GKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAYGDI
                     DIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGEEFL
                     GKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGYLEI
                     K"
     gene            complement(12305..12733)
                     /locus_tag="ACGI6B_003113"
     CDS             complement(12305..12733)
                     /locus_tag="ACGI6B_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9752530.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12885..13433
                     /locus_tag="ACGI6B_003114"
     CDS             12885..13433
                     /locus_tag="ACGI6B_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFG9752531.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            13552..14487
                     /locus_tag="ACGI6B_003115"
     CDS             13552..14487
                     /locus_tag="ACGI6B_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFG9752532.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14758..15702)
                     /locus_tag="ACGI6B_003116"
     CDS             complement(14758..15702)
                     /locus_tag="ACGI6B_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9752533.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         6140 a         2090 c         2004 g         5842 t
ORIGIN      
        1 tgatttctat agattaatag tttataaatt tagtaaatga atatttatta tagtctttaa
       61 caaaaaatac aagaataaac ttaattttaa taactaaaat taattatgca aaattattaa
      121 tatggtatta aattatgtat aaaaagttaa ttatgaataa aataattatg cctttgaaaa
      181 atcattaaac ttaaaattaa ttataaaaaa actctacctt actaactata cagctcacaa
      241 tctatctatt tatgaaaaaa tatgatataa ttgcattcaa aaaggaaatc tttcaatata
      301 ctagaattta agcattaact aattttatct aatattatta ctaaactaaa aaatatttgt
      361 atttgtagca ttggagcaaa taacttaatg tatattcatt ttaaatactt atttaaaatt
      421 tagcatttaa attatttaca aattaacatt taaatacttc tagcataaat acagttgtca
      481 tcaaaattta aagtatctta tggaggaata ccatgcaaaa tcataattat ataaaaatgt
      541 taaataaaaa ggtagaagta ttatctaagg aattagatag tagaaatttt aaattaaaaa
      601 ccttaaatga aatagatttt ttttctaaaa taccaaacag agaatttatg taccaacata
      661 ttgatgactg tatttcgaaa aatatacata taggaatcat gttattaaat atcgataact
      721 ttaaattatt taatgatata tatggatacc tagtcggaga aaatttatta attactctaa
      781 ctcaaaacct attgcacttt attccagaat caaagggaat agtcgcttat ttaaaaggag
      841 aaggtttttt aattgtactt ccaaatgtat caaataaaga gttaaataat atgggtgcaa
      901 ctattataga gagcgcccga gaattaaaaa taagacttcc taatgaaaaa gaaatatact
      961 ccaatgttac atttagtatc ggcagcacta tctggaatgg tgaaccaaac atgactactt
     1021 tgatgctttt caaccaagtg tatagtgctt taaaaaaggc taaaagtgaa ggtaaaaata
     1081 gatacattct atttgatttt aaagataata gtttttacaa taaattagaa aatgataagg
     1141 agttcttatc agaaatatca catgttctac aaactaaaca aattgaagta ttttaccaac
     1201 cactttataa tataaaaaat aatactatag ttggatgtga ggcacttgca agatggagac
     1261 accctattaa aggacttctt actccagata aatttattcc tttatttgaa cagtttggtc
     1321 tgataacgga attagatttg catttatttg aagagaactg taaaaatata agaagatgga
     1381 ttgataacaa tgatgttttt gttccaattt gttgtaactt ttctcgtctt cattttttta
     1441 accctaattt tccaagtatg ctaaaaggta ttactgaaaa gtacagcgtc tctacatcaa
     1501 attttagcat tgaaataaca gaaaatatat taatagaaaa tacaaataca attattcaac
     1561 aactagctga acttcgttct tatggttttt ctgtatcaat ggacgatttt ggttctggat
     1621 attcatcttt tggtatgttg caaaatctac cttttgatat aatcaaaatt gataggatat
     1681 ttttatctca taatttaaat gattttaaaa atacaaccat tttgtactct ataataaaca
     1741 ttgcaagagc tcttgggatg cttacagtat gtgaaggtgt tgaaacagaa caacatgtag
     1801 aatttattaa aagtataaat tgtgatatcg cccaaggtta ttattatgca aaacccatgg
     1861 agtgcaaaga atttgaaaaa ttactatcta aagagaataa ttcagagaaa atgtttataa
     1921 aatattctga tataggaaaa tctttaatag agaagatatt tgatggattt tttattatgc
     1981 aagatatgaa aaaactaaga gctaatgtca ctgaagacat tcaatggttt gatttttaca
     2041 acaaaactga aattacatct tttcctaaag taatagagca ttttgaaaaa catattaaag
     2101 gtaaaaaatt ttctattctt tacaaaagta taaccccaca taatactgaa tcagattcat
     2161 tattaattag tggagaagcc gttttatcag atgaagcttc tgaacatcaa aaatatagca
     2221 atttctactt tagtgcaaat tgtataattt cagaaaataa attactctta gcaaaattac
     2281 atttggattt aattaaaact gctggctata taaatgaatt tgaatcatat tcaataccta
     2341 aaatgacaaa tacacatctt gatgaaagta atattttatc acaattttat tcaatgttgc
     2401 ctgtaggtat aattcgctac gatttatttg gtgatatgat tattacttat atgaatgaag
     2461 aaatgtttga tattataggt tatacaaaag aacagttttt tggtgagatg ggcggaaatt
     2521 taagagcaat tgttcatcca gatgatttag actttgttta taaaaaaagt ttggacatga
     2581 ttgacacaaa taaaattgaa ccatttttat atagattcat aaaacgagat ggtcaaattg
     2641 tcacagttat gtatacacaa tgtaaccttt ctgcaattga tggaaggtcg attgcccagg
     2701 gtatgtatgt agatgtaaat aaaataaaaa atttaacaaa aatctagtac aaagtatttt
     2761 attttggaga aattaatatg tttaaagaaa tttttttaag aacttttcca ggatttctaa
     2821 ttataagaga ttctaactac agaattatat ttataaatga taatttaaaa aatttaatta
     2881 aaagttatat gcaagataat ccacttggaa tgactaatat agaaatagct aagaaactcc
     2941 ctgataatat tgctaaattt tttacggatt ctcacaatat tcaattagat tgggaaaaaa
     3001 attatcctta tgataaaatt tcaaattgga ttttagagtt taaaaaagat actacaagct
     3061 attggaatgt tttagaatac aaagtagatg ttgatgaaaa aacctacata attactatgg
     3121 ccaatgatat aacaaaactc tatgaagaaa ataagagaaa tcttcactac tcaattactg
     3181 acccattaac tggagcatat aatagaaaat atttaaatga tagatttgac atatttatag
     3241 gagattatat tgtactaatt gacttagata atttcaaaat gataaatgac tatgaaggtc
     3301 ataatgtagg agacaagatt ttatgtgatt ttgtttcact tttaaataaa gaattaataa
     3361 attctacttc tattatacga ttaggaggag atgaatttat tgttatattc tctagtgatg
     3421 ttgacaaaaa ttatgtttat tcacagctag aagccttaag agaaaatttt ttaaaagtat
     3481 tctctaagta taagtatctt tcatttagtt atggtgttga tacagtaaaa agaaatttaa
     3541 aattgacaat agttgaattg gataaaaaaa tgtataaaaa caaggagaaa aacaagaaaa
     3601 aatttgataa aaatgattat taacattgtt attttaaaat aaattaaaat tatctttctt
     3661 tttttaaact tgtacattag tttataaata tgtatctata caagatattg acattaagta
     3721 ctgaagcata tattgtttca gttctttttt gttaaaatta acttatttaa caaagatatc
     3781 atagtttaac aaagctttta atttaaatat attgatgtat aatataatta aaatataata
     3841 gcaaaaagaa aggaggaata tcatgagcat actcctaaaa aaagcaccca aattagcaaa
     3901 acatattata acaagttttt atataaatcg agatatcaat gaagtattaa aatacctttg
     3961 tgaaaatgtt acatggattg gaccaggaga acaggaattt ctcacttctt ttaatgaaat
     4021 aaaaaattat ttttatgcag gtcaagatga aattccttct tgtgatataa ataatgatat
     4081 atttgaaact gtaagtgaag atgagaatag atgtatggta ttaggaagat atactgttcg
     4141 aactaaagaa aatgctcaaa tgattttaga ggtaaatcaa cgttgtacat ttgagataat
     4201 tgaggataga ggaaaattat taataaagca catgcacata tcaaaccctt atggtgaaat
     4261 gcaacttgat gaatactttc ccactaaaat tggaactcaa agttatgatt atttgcaaag
     4321 attactaaaa gaaaaaacag aagttattga aatgattaca aataatatca atggtggctt
     4381 aaaaggtagc aatgatgata gtacatactc atttttctat gtcaatgagg gattgcccaa
     4441 aatattagga tacacatatg atgagttcat ggaaatgagt ggtggttcag cagtgggtgc
     4501 tgtatatcca ccagacttac ctaaagcact agaagattgt gaacgatgtt ttgcaaaagg
     4561 acctacatat tcatctgaat atagaatccg aaaaaaagat ggaacattaa tgtgggtatt
     4621 agattctgga atgaaatcgt taaatagtga tggcatagta aaaattaata gtattattac
     4681 tgacataacc caactaaaaa acatagaatc tgaactaaaa ttagaaagag agcgatatcg
     4741 tattgctttg caaaatatta cagatatcat gtttgagtat gatattgaaa acgataccct
     4801 tataaaatat caacgtgtag aaattgacaa aaaaattgaa ttagaaaatt ttgaaacaaa
     4861 aaattactcc aaactattag aatctggtaa aattattcac cttgatgata ttggaaaact
     4921 actagaagta cttcatggaa atcttcgtga aacaattgag ataagacaag taaattcatt
     4981 aactaaaaat gaatggagat ggattcgtgt tcaatgttca gttatatatg atagtgacca
     5041 taatcctatc aaaacaatag gcgttttaaa agacataact gaagataaat ctaaattaga
     5101 aagtttaatt aatcaggctc aacgtgaccc actaacacaa ttatataatc aaagagtatc
     5161 acaaaatctt attgaagagt atctatgtag ttctgattcc aaaaataatg atgctctatt
     5221 agttattgat attgatgatt ttaaaactgt aaatgatact tttgggcatc tagagggaaa
     5281 tgaagtttta gttgctgtat caaaaatact attacataac acacatgata aagacatagt
     5341 tgctcgaatt ggtggagatg aatttacaat atttataaaa tcattaacaa aggatttaat
     5401 aataaaaatt actaatgata ttttaaatga tgcgagtaaa ataaaagtaa aggacaatca
     5461 taaaataaca ttaagtattg gtattgcctt cactgatgat tcaactaaat tatataaaga
     5521 tttattttct aaagctgata aagctcttta tctatcaaaa gcagatggca aaaattgtta
     5581 tagtacatac gaataataaa gctatttata cgaccaagaa tgaaccttta aatttaattt
     5641 catagtctaa caatcaaaga gttgaagcta atcttatagc tagtcaacta gttcctagaa
     5701 aaaatttaaa caatatattt attgatactg ctcatttttc taaagagcaa atttatataa
     5761 aaaattaaac ttttatatac atacagatac taatgataaa cagttatcat tagtatcttc
     5821 tatcttaaaa caaaatgata atttatctat ataaaaaagt gctttgttga cagagttcac
     5881 ttttttatat aggtacagaa tttaagtata aatacatgtt ttattctata cattttagtt
     5941 aatgtaaaat ttcttgattg tactctctaa cttttcaaca ttatactatt tatataccac
     6001 ttagttaata taaaactata aaagtgttgc aattactggg ttttataaag ttaatttaca
     6061 taccacttag ttaatataaa actttagcac cttatttatc tatagagcta cagaaagtat
     6121 ttacatacca cttagttaat ataaaacatg tatttatttg ttcttgtact attgttttac
     6181 tctatttaca taccacttag ttaatataaa acaatatcaa aacaaataaa aatttttaat
     6241 aaatcattat ttacatacca cttagctaat ataaaacatt ctggtactgt tgctatttat
     6301 gttccgtatt cagaatttac ataccactta gttaatataa aactttggag ttgttacaga
     6361 ttatttgcct tatctctcta tttacatacc acttagttaa tataaaaccc ccaaataaac
     6421 tgggcatttt caatacttac acatatgcaa ctctcttaaa tttgcagtga accatgagta
     6481 gtgcaaatga taaagcttat catataccct caacacctta tatttcaagc gttaagctct
     6541 actttgtaac aaatatcgct cactgcaaaa ctatttacat ttttattata tcataaatat
     6601 attatttttg aatatctata ccaatttgtg gtataataaa agcaagaaga actacaatct
     6661 atttagacgt agagtgaagt tcaaatacta atgtttattc tttttgaact tgatttttag
     6721 ttcaaaacta aagtcactcc tgcctgtgtg aactataccc gctgttttat acagttaaaa
     6781 catgtaaaaa tagttataaa gtactagtgt aactcattag tactcttttt attatattca
     6841 tataattaat atctatattt taaagcttac tatctaaaaa agagcacgtc aaataaaacc
     6901 ctagatattt aattttttta gagatactta aaataaatta tgctctctct gtacttaaat
     6961 atttcctaaa ttcaaatgga gacatagaat ttaatctatt ttgatatcta aaattattat
     7021 agtagtatat ataatcatct attgctttaa ctaattattc atatgtatca aatttcctta
     7081 aatagtacat ttctaccttt aaaattcccc aaaatccttc cataggagca ttgtcaaggc
     7141 aacgaccaac tcttgtataa tatatgtata tctaacacca ttaagaaata tatattttat
     7201 aatatatatt tcttcattta atgcacttag aaaagcaaag aaaatacaga ttagtacact
     7261 ttttagagaa aaataaaatt aatacatcta aaatattaaa aaatacttta gataatctaa
     7321 agacacatct caaagttttt aactaaatta taaaactaat tacaaatgct atgtaacgat
     7381 aataaataaa aaatttactt cgcttaagcg acgtgtcgtt gaaatatttc atgttgccaa
     7441 ctgattcata agtcgataag aaaagaacac taattattta gagttctttt cttagctaca
     7501 agtggattat aatactttta aatctgtgag aaaatcatat ataataatga tatgaacaag
     7561 taaagaaaat acaaaaatga caaaattata aataaaatat tataaaatca taaggtcata
     7621 attgttatat tgtagaaaca aagtaaagag agagggtgga gaataaatga atcaaaaaaa
     7681 aataaagatt agtattaaaa tggtaattat aggtatattt tctgtaatta ttgcttttgt
     7741 attatccaga ttattcgttg agtatattga tgaattatta aggtctaatt ggcaatatca
     7801 gctatttgaa agtgagagtt catatataat atttgccaat tgtattatgt tctcgaatat
     7861 attattagaa atatttttaa tatatatttg tagaaaattt agaaaaatct aatatggtca
     7921 acgtatttcg aatcgaatac ttcatgatta tttagtctat tgtgctagtt aaagtttaat
     7981 tcccatataa agtaaattga ttttagtaaa acaaatcacc cctaagggtt agtttatgga
     8041 taagtcatcc aaatattaac tctttaattt ttgctataga ttcatctttg ctcataactt
     8101 tacttataaa aaatgaaata ttataagcta gaacttttat tctaattcta gtcataaatc
     8161 ctagcataga gttacttttt ttatattata aataaaacta tacaacaaag ataatattta
     8221 gtaaaaataa ctttcatttt aactaaactt aaaattcaat tactctctaa attgacgttt
     8281 aggaattagt tcaaaataaa aaagagtgta tatattgaaa ttactacatt tcaatatata
     8341 tactcttttt aaatttctca gttttccatg gaactttact ttatttattt ctgtattagt
     8401 tgtatttttt atgcattttt ctttaaaatc atattttctg attcaactct tatattataa
     8461 aatttcttta ctaaattatt ctctttttct atatcttcat atatattttt taatcgcctt
     8521 ttacctaatc ttttatctaa cattactaat gatttaacta atatattata tgattttatc
     8581 gcatcttcta ttgagatatt taaacatata tttactgctt cataaaaatc aaattcatta
     8641 aaaaatccat ttttatttac aatcttatat ccctttgcat ctctttctaa atactcttcc
     8701 taaatactct tcaaaacttt catcatctaa tctaaatcct atatcactat gaaaaatagg
     8761 ttttgcattt ggatatagtt ctattgtttt atcaaaagtt tcgaatacca agttattatt
     8821 attcgatttt ccaatagtcc actataaaca ataataagtt tgttgctcca aataatgtaa
     8881 cacttgtaga aagatgttac agatatataa aagatagaga atgtagtttt tctcctaata
     8941 ctattagaaa tagaaaaaat atgattaaaa atcaaattga agttttcttt aaagatatga
     9001 aattaataga tataacacct tctattttac agtcttatat aaataacata tacaatgagc
     9061 acatgttaaa ttcaataaaa aaccaagtcg attttataaa atcagtattg aaagaatctt
     9121 atagattaaa agaaatatca gaaaatattt gtgattttgt tacaacccca atcaaaaaag
     9181 tagttcaact tctaagcttt atacaaaaca aaaagctcaa ttattattag aaaaatcaaa
     9241 aaatattcct ataggtatac caattttttt gatgctaact cttggattac gttttggtga
     9301 agctgtatca cttatatggt cagatgtaga ccttgataaa aaatctcaca tgtaaatcaa
     9361 acattagttt atgtatacaa taaaataatc ttcaaagacc ctaaaacccc tatatcaaaa
     9421 agaaaattat ttgctcctga tgaacttata agtttgctta aaggggaaaa attaaaacaa
     9481 aataagttaa aattacaggg tatattgaaa aatgaatttg atttgatatg cttaaataaa
     9541 agattcaacc cgtgagttca accaactttt gttgttcatt ttaaaaaact attaagagat
     9601 aataatccta gagatataaa agtacatgaa ttaatacatg caaatgcaac tttgatatta
     9661 ctttcaggta ctaacataaa agctatttat aaaatattag tacatgcaga tataaaaata
     9721 gctatgaata tatattccat attctcactt attagatgaa atgaataaag aaacatctga
     9781 aaatataagc aaaatactat tcaaataatg ttcagtttta tgtcaattaa atgtcactca
     9841 aagtgctttt ttttcatttg tcagttaaat gtcacttaaa gtaccttttt cctatttgtc
     9901 agttaaatgt cagtaatata taaataagat atatttataa gacccttaaa atcactatta
     9961 tttaataaaa tttataatta tatactattt gctgttgaca ttggtattat accatagttt
    10021 atcatttata ttagaaataa aattattaat taatagtttc gtaattttat aatagattta
    10081 cagggagtga tagtaaatgg atacaaaaat atctataaac gaaaacatga gtctaggtaa
    10141 aagatttttt aaatacctag caccatctgt agttgcaatg tgggttttct cactatatac
    10201 aatggttgat ggaatatttg taagtaaagg agttggagaa ttagctttag ctgctgtcaa
    10261 tatatcaatg ccttttatta attttatatt tgcagtttct ctattatttt caactggtgc
    10321 atctaccata attgcaatct atctggggaa aaaagatatt aaatctgcta acgaagtctt
    10381 ttcatttaac ttagtatcaa taattatatt atcaataatt atattagcta taactttttt
    10441 taatttagat agattagcac tatttttagg agctacagaa tctacaattg gtatggttaa
    10501 agattattta ggaataataa tattttttaa cggattcttt atagtgtctt attctttaga
    10561 agttataata aaaactgatg gctttccaat attagctact gttggtgtaa taatatctgc
    10621 tcttacaaac atcatacttg attacttgtt tgtaattgaa tttggatggg gtgtaaaagg
    10681 tgctggtatt gcaactggtt tgtctcaagt cttttctact attttcttct taatacattt
    10741 tttaagaaaa aactctactt tgaatttttc aaaatttaga attgatttta agacacttag
    10801 aaaaattgta tttataggtt ttcctgattc aactactgaa ttaagttgtg gtatagttgt
    10861 tttacttttt aacttaagtc ttactaaata tattggagaa aatgctctta tttattatag
    10921 tgtcataaat tatataaata cattagtact tatgactatg atgggtataa ctcaaggtat
    10981 gcaaccactt actagctttt attatggtgc aggaaatata gataatgtaa aaaaattatt
    11041 aaaaatgggg ataaaagcca caataatagc atcagttgct gtatttgcaa tttgtatggc
    11101 tttttcagga ccaatagtct ctttatttat acatccagaa gaaacaatgc tattcaatga
    11161 aggagttcgt gtatttaaaa tattctctat atctttctta ttggtaggaa ttaatgtaat
    11221 aatttctgga ttctttgtat cagtagaaaa accatcaatt tctactgtta tatctcttgg
    11281 tagagggctt gtaatagttg ttttaagtct aatttcaatg atactaatat ttggtggaca
    11341 aggaatatgg atgactacta ttgtttctga gtttatatgt cttattttat cattagtttt
    11401 tctaaaaaag aatttttcta cattagactc taatctaaat aaggttgctt agatgataag
    11461 agtgacaata tatgaaaata tctaaataaa aaatacctat tatacaatct tgcaaatatt
    11521 aaacctttgc aaaaattagg attatataat aggtatttta tttatgtaat tattttattt
    11581 ctaaatatcc atagccatct tttattaaag ttccttttac ttcatattct acaggttttt
    11641 taaatattgg accatctacg acaaatgtat gatactctcc atgttctcca catgggtctg
    11701 caccagcatt ttttatcttc tccacaagtt ctctagttaa tttttttcct aaaaattcct
    11761 ctcctaagtg ctttaagttt acttttgtta caacactaca aaatccactg tctataaatt
    11821 catatactaa ctcctctcta ctctcttgcc ataatggtaa atctactttc ataccaactg
    11881 cttcacatct atcagtatcc cattttctat gactttctat atctatatct ccatatgcac
    11941 atatagttgc tcccatgttt ttagctttta ttaaagccct ctcaaagctc tcttcatatc
    12001 cttctacacc acactcagca actaataaag gtaatcctat attgctactt acttgcttaa
    12061 gcatattata ttctaaatta tgtgtccatg actttccatg ttcttcactt atagttgtca
    12121 agagtgccac aggcttatat ccttttttca acattcgatt taatgctaat atactatcct
    12181 taccaccact aaatgacatt acaaattttt catcagccaa ttttaacacc tctttcatct
    12241 aactttgttt tattaacata attataccaa atttttgagt tgcatagcaa attatattaa
    12301 attgttaatt cactataaag aatgtcccta atattaaaag tataacccct atgtatttgt
    12361 ataaatgcat tgtttcttgt cccaaaatta cgctatcaat aaaaaatcca actaaaactt
    12421 gggatacaag agttaatata agtgtatttg atactcctaa atttggaatt gcctttactg
    12481 taaaatatat tattagacct ccaaatactc ctcctaataa caacttataa ttaaaaccat
    12541 ttatagatac tagacacttt aagtcacctg taactattat acttgccaaa aaaaatattg
    12601 ctcctacagt taagcttata aaggtggcca ctaatgctgt tgtacttttt cctagctctc
    12661 catttataaa agcttcaaga gctgtagaac cacctgctaa taatgcaaat attattgccc
    12721 ataattttgt catggacaaa cctccttcta tataattcta ttaatagaag tttatgatac
    12781 aacctattaa ttaattacta agtatagttt aatttttata cactaggctt tatttggata
    12841 tgaatttctg ttataattgt tctatcgatt ttggaggtga ttttatgtac acaaatttta
    12901 atatcgattt taataacttt aataaaaaag aaaatgccat taaatttatg ttaatgggag
    12961 tattactcat tatattagga ttattatgcc ttacatttaa gacattaggt ataaaattaa
    13021 tttcttggac ttttggtatt gcacttttat tctttgcata tttaaattta aaaaatatta
    13081 atgaactaaa aagatatgct actaaagaag aaattaaacc ctctattaat atccaatggg
    13141 ttctaataat tgcatgtata ttactttttg tatttcctca aaaaatacaa agcatatttt
    13201 cattattttt aggattttat ttaatcttta atcaactagt agcacttgta aatagtaaga
    13261 ataatcctta ttctaaattt acaacttgga atatagttaa gattctattt ggtatatgcc
    13321 ttatattatc tccacttttt ttatctagat ttatagtatc gataatgtct tttttcataa
    13381 tattatttgg actagttcta tttttctctg gaaatacagc gagaaaatat taattatttt
    13441 ctcgctgtat ttttatatta cttaaaatag aatttgttta agttatctat ttgtggtaat
    13501 atgttttttg tacaagctca gcgtacaatt ttattagaaa taggtgattt tttggataaa
    13561 tttaaatatg cttttgataa taaaagatat catacttgga attactatct tagaaatact
    13621 tttggagaaa aagtttttaa ggtatctata aatgcaggat ttacttgccc aaacattgat
    13681 ggaagtcttg gttatggcgg atgtacctat tgtagcaaag aaggctcagg tgattttgct
    13741 ggaaacccta aagataatct tatcagtcaa ttttataata taaaagaaat gatgttaaaa
    13801 aaatggcctc atgcaaagta tattggctac tttcaagcct atacaaatac gtatgctcct
    13861 cttgaagttt taaaagaaaa atatgagact atattagaac ttgaagatgt cataggtctt
    13921 tcaatatcca caagacctga ctgtttgcca gatgatgttg tagaatactt gtctgaacta
    13981 aataaaagaa ccaatttatg ggttgaactt ggacttcaaa caattcatga ttctacatct
    14041 aaaattataa atagaggtca tgattataaa acatttgttg aaggtgttga aaaattaaag
    14101 tctaaaaata taaaggttgt cgtacacatt ataaatggtc ttcctggtga agattataat
    14161 atgatgatgg aaacagcaaa ggcagttggt aaaatgggtg tagacggtat aaaaatccat
    14221 ctattgcatg ttataaaaga tactcctatg gaaaagatgt tacaaaatgg tatgttaact
    14281 cttatggaac aagatgaata tatcaatctt gtctgtgacc aattagaaat acttcctgaa
    14341 actatgataa tccatagact cactggtgat ggtaagaggg atgaactagt aggtcctata
    14401 tggagcctaa aaaaatggga aattttaaat aaaattgatg atactttaaa ggctagaaat
    14461 tcttatcagg gctgtaagtt tgtttaatac aaaataataa ctatagaaat ggttatacaa
    14521 atatagtcct ttctatagtt atttagtatt tacaaaacaa aatattaatt tgtacattta
    14581 tacaatatat aataaaaaat agatagtcta aactgtatct acttatattt tatttgtact
    14641 gtccacttta ttctataaaa tttataaata gttatttttt aataccaaat ataatttggt
    14701 ataatttttg ttggaatcta caatgtatac caataaatcc tttttaagta tataattcta
    14761 aatactttca gttttttcaa tctgctttaa cttgttggac ttataaaata ttcttatttc
    14821 atcataagaa agattaagtg ctactactgc agatattaac atcaacatca ctccaaataa
    14881 cttccctaag gaaaaacttt ctcctaatat agtgcaacca attattactg aaccaactaa
    14941 ttctactgat gccacaacac ctgcaactga caactctaca cctttagata tacctgctgc
    15001 ataaaaaata tatgcaagtg cagctggaac aatgcctaac attagcatac aagataaaac
    15061 atctaaatcc ttagcataat ttaagatttc ccaaggtctt gaagaaggaa tcataaatat
    15121 agctccaaat aagaaactgt atactaatat tgttgaactt gaaaattcct ttaaagcatt
    15181 tttactaata gttggcataa gtgcatatgc tattgctgat aatactccaa gtaaaagccc
    15241 aaaagcgttt aaaccttgaa aatccaaacg tccaccagtc actgccataa ttgcaccaat
    15301 aaaacaaaga ataagtgaaa ataatttgct tctagtaata ttttctttat aacataattt
    15361 tgaaaatatt gctaaaaaca gtggtgatgt atatagtaaa actgctgcaa ttgatacacc
    15421 cgcaatgtca attgctttaa aataacataa attaaacatt gcctgacata taattcctat
    15481 tattactgaa tatataattc cttttttgtt tatctttaag atttgtggtg tccttataga
    15541 tgaatatacg cctaaaacta taaatccaaa aaacaatctt gtaaatgcta cttgttctga
    15601 tgtcagtcca tttcccatta aaaactttcc aaataatcca agtgtagccc ataataaccc
    15661 tgctattgct ataaatacat accctttact tttttctccc attttaaatt cccttctatt
    15721 tttatttagt caatttacac tcttcttttt aagcaaaaac tatgccatct taaatcaata
    15781 tatatcgact ctaaaaccct agtctttcca ataatactaa taaattttca atttttttat
    15841 atttattttt cgcttacttt aagttgattt agacttattt taattgtaaa atgagttata
    15901 tataacttat ttaaagttaa aatagactga aacttatata taaatatcaa attttaagta
    15961 tattaatcaa ttaaacctat tttaatatat cattaaaaat ttaggaggaa aatataaaaa
    16021 tgaatttaga tttggaattt tatcaaaaaa tattagaagc ttcccatgat gaaata
//
LOCUS       DAYAOY010000097        15442 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYAOY010000097 DAYAOY010000000
VERSION     DAYAOY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.097"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            135..3038
                     /locus_tag="ACGI6B_003117"
     CDS             135..3038
                     /locus_tag="ACGI6B_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9752534.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3071..3517
                     /locus_tag="ACGI6B_003118"
     CDS             3071..3517
                     /locus_tag="ACGI6B_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9752535.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3640..3942
                     /locus_tag="ACGI6B_003119"
     CDS             3640..3942
                     /locus_tag="ACGI6B_003119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9752536.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4123..5529
                     /locus_tag="ACGI6B_003120"
     CDS             4123..5529
                     /locus_tag="ACGI6B_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9752537.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5873..6931
                     /locus_tag="ACGI6B_003121"
     CDS             5873..6931
                     /locus_tag="ACGI6B_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9752538.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7030..7716
                     /gene="araD"
                     /locus_tag="ACGI6B_003122"
     CDS             7030..7716
                     /gene="araD"
                     /locus_tag="ACGI6B_003122"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFG9752539.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7734..8381
                     /locus_tag="ACGI6B_003123"
     CDS             7734..8381
                     /locus_tag="ACGI6B_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFG9752540.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8554..9921)
                     /locus_tag="ACGI6B_003124"
     CDS             complement(8554..9921)
                     /locus_tag="ACGI6B_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9752541.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10111..10199)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10627..12747
                     /locus_tag="ACGI6B_003125"
     CDS             10627..12747
                     /locus_tag="ACGI6B_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFG9752542.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12851..14104
                     /locus_tag="ACGI6B_003126"
     CDS             12851..14104
                     /locus_tag="ACGI6B_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HFG9752543.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14258..15319)
                     /gene="ytvI"
                     /locus_tag="ACGI6B_003127"
     CDS             complement(14258..15319)
                     /gene="ytvI"
                     /locus_tag="ACGI6B_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFG9752544.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         6131 a         1752 c         2666 g         4893 t
ORIGIN      
        1 aaatgtaaat acttatcaaa atgtataaat acttatgaca tctagttact tacagggttt
       61 aagtagaaaa aaatatcaaa gtaagtatgg catgatagtt gcttatatat ttatcaagaa
      121 aggagttatt aagcatggag ttgagagata aacttgtaaa catcataaat acagaaaata
      181 aaaaagatcc attaacagat gttcaaatag caaagttttt aagtacaact agagagaaca
      241 taactaattt gagaaaagaa cttaatataa gtaattcaag gcaaagaaga tatccttatt
      301 taaaatcagc aatttcggca atattacaaa agaataagaa tatttctata tcagaaatta
      361 caagggaatt gatgacagaa ggctttaata tatcgagaag agttgtggag gaacttttac
      421 ctaaagaaag tttagaaatg gatgtagtag aggaagatga agaagaatcg aatgacccat
      481 ttgaaactct gataggtaat aaagggagtc ttagaaatgc tgtagagcag gctaaatctg
      541 ctatattgta tccaccaaaa gggttaccaa cgttgattat tggggaaagt ggagttggta
      601 agagtttatt ttctagacat atgtatgagt ttgcaaagca aaaaaagata gtaaaagaga
      661 gctccaattt tgttgtattt aactgtgctg attatagtga caatccacaa ttattattat
      721 ctttgttatt tggatataaa aaaggtgcat ttacaggtgc tgaatatgat acaccaggtc
      781 ttgtagaaga agctgatgaa ggagttttat ttttggatga aatacataga ttgcctccaa
      841 aaggtcaaga aatactattt tctatcttag ataggggaaa gtttaggaga ttaggtgaaa
      901 ctaatgctga aagaaaagta agtattatgt tgattggggc aactactgaa aatgtagaga
      961 ctaatttatt attaacattc agaagaagga taccaatgct aataactttg ccaccattac
     1021 atgatagatt actaaaagag aaaatagacc ttatatacaa tatctttcaa caagagtgta
     1081 atagaataaa tgcaaaaata tttgtagata aaaatgtgat tgaaatactt gcactaaaaa
     1141 agtttagtgg aaatatagga caattacaaa atatgattca ggttctttgt gcaagagctt
     1201 ttatgaagtt tataaattcc aaagatgaag atagtgaatc tatagtagta gtagatataa
     1261 atgaagtttt aaagcttaaa gattcgttta aagatgctgc ctttcaagag attgaatata
     1321 cagagataag aaaatatttg aaaaatgcta tatttatacc atttaattta gaagaatcat
     1381 cagtaaaagg aagtctttta agtgatgaat atatattacc agaggacata tacaaaacaa
     1441 ttgaaaagaa atattatgac ctaaaatccc tagacattga agatatagat atagagaata
     1501 ttttatggac ttttatttta aatagatttg ctgacttaaa gtttaatctt gatagtaatg
     1561 aagaaatttt ttcaatgagt gatttaaata gttttgtaag tgatagttta gttaaactgg
     1621 taaaagaact tatgaatgat atagttgaaa aaaatatgaa tttagaagta aataaaaata
     1681 tgttcaaata tctagcaata cacttagaag aagctataaa aagaattaag ctaaatcaaa
     1741 agataataaa tgtaaattta gaaaaaataa agattgactt ttcaaaagaa tatgaaatta
     1801 gtaaaagttt tgcaataaaa attgaggaat caattggtat taagatttca gatgatgaaa
     1861 ttggatttat aacgctctac ataaagtctg ctctaaaaaa tgaagtgaaa aaagataaag
     1921 tagggttaat aataatttct catggacgta tagctacaga gactgtaaat gtagttaaag
     1981 aacttttagg tgtaaagttt cctgtggcta tagatatgcc tttagatgaa aaaccaataa
     2041 atatatacaa taaggcagta gaattgtcaa aaataataga ccaagggaaa ggcatattgt
     2101 ttttggtgga tattggttca cttacaaaca taggtcaaat agtaaataag agaaccaata
     2161 taagtactaa aactattgat agagtagacc tcctaatggc actagaagca actagaaaag
     2221 tttctatggg agaggaagaa cttgatgaaa tatttttttc tttgctaaaa gataggatgg
     2281 gatataacta tgaattagaa aaacacatag ataaatcaaa tgcaattata acattatgtc
     2341 ttactggaga aggaactgct aagtatataa gtaaaacgtt ggaagaaaag tatgataaca
     2401 ctaaatgtta tcaaatgagt gcattggatg aaagtttatt ttgtaaaatt gaaaatctaa
     2461 aagagacaaa caatatatta gctattgttg ggacaataaa ccctaagata ccaggtataa
     2521 attttatacc ctataataaa gaacttttca aaaatttgga tatctatcta tcaaaatcaa
     2581 aacaacaaga tgacagacca attaaatcgt atgaaagaat gcttgatgag gatttagtga
     2641 tttttgaacc agatatatat tttaaaaagg atttattaga atatgtatgt tcaattctta
     2701 taaataaaga atatgtagaa aaagattact tagattctgt tttacataga gaagaaatgc
     2761 ttccaacata ttctaaagga gctataggtg tgcctcatgg agattcttct actgtaaata
     2821 gtacaaggtt tgtttttgtt aaactaaaga atcctattga ttggggtgta ggtaatgtaa
     2881 atttcatatt tatgccagtt tttcaggcta atgataaaga gattgttaaa aatgttcttg
     2941 agatactaaa agatgcagaa tttatgaaca atgccaataa gtgttttgat aaagatgctt
     3001 ttagaaggat tatatttgat aaatttaaac atctgtagcc atagcaaaaa aattgagagg
     3061 agttagagta atgaatgtag atttaatatc attgggttta gaatctaatg attatgaaag
     3121 tgttattgaa gaattaggaa gtataatgtg taaaaaagaa tatgtaaaag aaacatacat
     3181 aaatgctgtc ttggaaagag aaaggacatt accaacaggt ctagatattg gagacatgtg
     3241 tgttgctatt ccacatacag attcaaagca tgtaaatgag tctaatgtag ctgtagggat
     3301 attgaaaaat cctgtaaagt ttaattcaat gatagaccca aaagatagac ttgatgtaga
     3361 acttgtattt ttattagcag taaagaaccc agactcacaa gtaaaactgt taaaagattt
     3421 aatgtcagtt tttcaaaata taaagttatt aaaaaatata aaaaacgcat caacaaaaga
     3481 agaagtagct aaattattgg attttataga aatttaatgt aggagaattt tataaataaa
     3541 aaattaaaat ttaaacaatc aaagtaagta ttattttttt aataggttta atcttatata
     3601 aaataaaaat ttagagtgaa aaattaggag gaaattatta tgaaacaatt taatgtatta
     3661 tcagtttgtg gttcagggac agttacatca tctatggtag caggtaaatt aaaagaagta
     3721 ctaggtaata aggggatttc tattactaca acagaagcta gaccaacaga agcactaaat
     3781 ttagcacaat caggaagatt tgactttata acatttacga gtccacttca aacaggcgat
     3841 tatggaatac caactataaa tgcatttgct tgcctaactg gtatagggga agaggcattc
     3901 tttgaagaag ttttgactgt tattgaaggg atagataagt aagttttgga gacgtgaatt
     3961 tatagtattt aaatcaatga tttagatact ataataagct aatattaact aaatatttgt
     4021 ggaaaaaaca ttttatatgt tgtcataaat taaaaaatta taaaaatagt cacttaagta
     4081 ttaaattaag cttataaata tttataagta ggagggaaaa agatgttaga atttatgaaa
     4141 tctataatcg atacatttgg ttctgcaatt atagttccaa ttataatatt cataatcgca
     4201 aagatattta aagtaactac taagaaatcg tttctttctg ctgtgtatgc aggagttgct
     4261 ttacaaggtt ttacactgat tttaaattca tttacaccag taataacacc agttatcaat
     4321 aggatggttg agagtacagg agtaaaccta ccagtttttg atgttggttg gcaagcaaca
     4381 tcattggttg cattttcaac aagtgcaggt atgatatatt taggtcttgg tatattatta
     4441 caaacaattt tatttttgat aaaatggaca gatgtgtttc aaccatcaga tttatggaat
     4501 aactattctt atatggtatg gggagcaatg gttataggag taacaggaaa ttttccttta
     4561 ggtatagcat gtatggtttt attaaaccta tatagtttat taatatcaga gttagttgca
     4621 aagagatggt ctagttacta tagatatcct aattgtacaa taatagctat gcataatgtt
     4681 gaggcttctg tgtttgcagt atttgcagac cctatataca ataaattggg attaaataaa
     4741 ataaaattaa atccaaaaga attagagaag aaattagggt ttttaggtga accaattact
     4801 ttaggtcttt ttttaggtat gtttataggt atattaggta atatgacgag aataaatact
     4861 atggaagctt ggggagaaat aatgaaagta ggtatatcta caagtgcagt gatggcaatt
     4921 ttccctaaag tagcaagtat gtttgctcaa gcatttgcac ctataacaga agcagctaga
     4981 aagattatgc aaaaagctgg aaaccgtgag tggtacatag cagtaaatga tgctgttgga
     5041 tatggtgaac ctgcaacttt aatatcaggt cttatattaa ttccaataat gcttgtaata
     5101 gctatggtac ttcctggtaa taaggtatta ccagttgtgg atttattggc aattccatat
     5161 atggttcaag gtttagttgc catacataat ggtaatatac caaaagtatt agtttcaggt
     5221 ataatttggt ttggattagg attatatgtt tgtacatcta cagcaccatt atttacagat
     5281 atggctacaa atataggtgt agctatacct gcaggagcaa tgttaataac aagttttaat
     5341 atactaggaa aacctttgat gggattagtt ttctttgcat ttctaagtgc aaatccaatt
     5401 tatataggtc tttcagttgt aatttacttt gttctatggg cattgtttag aaagaacaaa
     5461 acaagtattc ttgattattt agaaaaacaa gcacttaaaa atgtagaaga agagcctgta
     5521 gcagtttaag tttataaaaa tgaaataaaa ataattaaag gagtttataa ttgattttat
     5581 ataagtatca gataatacct gagaaatatt taaatgaaat atagacttct ttttatttga
     5641 taatatttgt aattataatg cattgtattt ttatagttga gttttctgta taacctgcta
     5701 tttaaaacat actcttttat atttatacag taatgtggtt taattttata aaatgtaatc
     5761 tctactggta aaagactttg tatataaggc atttgcctga ataaaccatt tatcttttgt
     5821 atggcataga tattgctata tagaagtata tagcatgaaa ggagatattt atatgggaaa
     5881 taaaatgaga gcatcagtat tgtataatgt aggtgatgtc agatatgaaa tggtaaatat
     5941 accagaaata acagatacgc aagtgcttgt aaatgtaaaa tatgttggga tatgtggttc
     6001 agatttacct aggtctatgg taagtggttt gagtggaaat acaaaatatc cattgatttt
     6061 agggcatgaa ttttctggtg aagttgttaa aattggagaa aaagtaaagc atattaatgt
     6121 aggagataga gtagctgttg caccattagt accttgtgga aaatgtgatt attgtaatga
     6181 aggtaatttt ggcttgtgtg atgattacaa tataattgga acaagagtaa atggagcgtt
     6241 tgcagaatat gtaagagttc cagaagaaca tattttaaaa ctaccagata cattggatta
     6301 tgaaactgct gccggaattg aaccagcaac tatagcttat catgggattt caaagtctaa
     6361 tattagagta ggagatagtg tagttgtgtt aggatgtggt ccaataggtc agtttgttat
     6421 acaatgggca aaggtgtttg gtgcatctaa gattatagca gttgatattt ttgatgaaaa
     6481 attagaactt tctaaacttc taggagcaaa ctatatactt aactcaaaag aagtaaatgt
     6541 aatcaaagaa ataaaaaaaa taacaaatgg tggtgctgat gttgtaatag aaactgctgg
     6601 tagccgattt acacaagaac aatctctatt tgtagcaaaa aagagaggaa atatagtgtt
     6661 tgtaggtata tcacacacag aattaccact tagtgcagac gctactgagt gtatattacg
     6721 tggagaactt accttgaaag gttcttggaa ttcatataca agcccttatc ctggaagagc
     6781 atggactgca acattggatt ttatggaaaa aggtgacatt atttttaaac ccatgatttc
     6841 agataaaata ggtcttaatg aagtgggtga ctttttaagt aaaatgagca agagagaaat
     6901 taatttcaat aaaatcctag tagaaatata aaaatttaat aaaaaattct aaaagcaatt
     6961 aaattttata actatgacat taaataaaat aaaaataatt ttcttaaata aaatatagga
     7021 ggaagaaaag tgttagaaaa tttaaaagaa gaagtattaa aagcaaactt agaattacct
     7081 aagaaaaagc taattactta tacttgggga aatgtaagtg ggatagatag agaaaaagga
     7141 ttagtggtga taaagccaag tggtgtagag tatagtaata tgactgtaga agatatggtt
     7201 gtagttgatt tagagggaaa tatagttgat ggcaaattaa gaccttcttc agataccccg
     7261 actcatctag ttttatataa agagtttgaa gaattggggg gaatagtaca tacacattca
     7321 agttgggcaa caatttgggc tcaaatagga aaatctatac catcttgtgg tactactcat
     7381 gcagattatt tttatggaag tataccatgt accagaaaaa tgactagaga agaaatttgc
     7441 aatgaatacg aaaaggaaac tggaaatgta atcattgaag aatttatggg aagaaatcca
     7501 attcattgtc caggaataat tgtaaatgac cacggacctt ttacatgggg aaaagatgca
     7561 aatgaggctg tacacaatgc agtagtttta gaagaagtcg caaagatggc ttattataca
     7621 gagttaatga gtcctgataa tattatggat aaggttctta tgaataagca tttttcaaga
     7681 aaacatggaa aaaatgcata ctatggtcaa aaatgattat ctggaggtga attatgctag
     7741 actctttaaa aggtaaagtt gtggaaatag caattaaagc agagcattat ggtttatgta
     7801 aagaaaagac tggtaatttt agtattagag ataaggatac tggatatata cttataaccc
     7861 catcaggtgt aggaagagaa gaattaaaag ttgaacatat ttgtgtattg gatttggatg
     7921 gaaatgtaat agaagttgag aaaggtatta agccaagtag tgaggtattg atgcatattg
     7981 aaatatacaa aacaagagaa aatataaatg ccatatcaca tactcattct ttatatgcaa
     8041 cagcattttc agtagcaaag aaaccaatta taccaattgt atatgaatca gttaattatg
     8101 gcggttatgt agatattgct tcatacgaaa gaccaggaac tttaaaatta gctaagagtg
     8161 tttcaaaatt actgatagat acagatgcat gcttattaga aagacatggg ttgacaactg
     8221 taggtaatga tttagacgaa gcattattaa aatcaagata tgtggaagag gtagcagaaa
     8281 tatactatag gtctttagta ttaaatcaat ttaaagaact tgaagtagta gatgttgatg
     8341 agtttaaatc ttggaaatac ccagaagaaa taaagtttta atctaaataa ttttcattga
     8401 agtggttaat taaaatatat gtattattta tatcaattac tatgttttat tatgttaatt
     8461 accgtatttt gcaaatatat ttattacatt ttttcaaaaa aatatagaat ccgaaagcca
     8521 tataaaattg acttgggatt ctatactttt tatttatgca tttttcttta tctgattttc
     8581 tggagaattt atatgttcaa aatatttttt agtttcagat actattatag gtgataatgc
     8641 aagtaaagct ataaggttag gtattgccat aagcccattt acaatatcag caattatcca
     8701 aacaacttct aactttaaga aagcacctaa taacaccata gccacaaata cagttctata
     8761 cccattcatg ccttttacac caaacagaaa ttcaaaacat ctttctccat aataactcca
     8821 tcccaaaatt gtagtaaatg caaataatgt taaactaact gtaagtaata ttggtccaaa
     8881 tgtaggaatt gctccgttaa aagctgcttg tgtcataaca gctccattta ggtctgaaca
     8941 ccacactcct gaaataacaa gagagaatcc agtaagagta cagattataa tagtatctat
     9001 aaaagtacct gtcatagata taagaccttg ttccgctggc cattcagttt tagcagcagc
     9061 tgcagctatt ggagcactac ctagaccaga ctcatttgaa aatacaccac gagcgatacc
     9121 atttctaatt gccagtgaca tagttgctcc taaaaaacca ccagtagcag cagtaggtgt
     9181 aaatgcactt ttaattatta acataaaagt ttcagggatt ttattccaaa aagatattaa
     9241 aatgattcca cagattacaa catatataac tgccataaaa ggtactattt tagtagctac
     9301 attagatata cttttaagtc ctccaaatac aactaaagct acaaatactg ctaatacaac
     9361 ccctgtaatt ctagggtcta tcccgaaact attatttatt gcatctgtta ttgaatttac
     9421 ttgagtaaaa gtaccgattc ctaaaagtgc aactagtata ccacttatag cgaaaaatat
     9481 tgcaagaggt ctccattttt ctcccatacc atttactatg taatacattg gcccaccaga
     9541 cacttgacca tttttatctt tagttctgta ttttattgca agaacacctt ctgaatactt
     9601 agtagccatt ccaaagaatg ctgctatcca catccaaaaa agtgcaccag gtccaccagc
     9661 tttgatagct gtagcaacac caacaatatt accagtacca actgtagcag ctagagcagt
     9721 acataaggct gcaaaacttg aaacatcacc actacctttg ttttcagcag agaatattaa
     9781 cttaagagct cttggcagtt tagttatttg aacaactcca agcttaacag taagtagtat
     9841 accagtacca actaatagta ccagtagggg tggtccccaa ataaaagcat caactttgtt
     9901 taaaatatca attagattca taaaaacact ccctcatttt ttttgcttta cataatcaat
     9961 agaattttta atgaagtaat tgacaggaaa taagtataat ttattaataa tttctagtgt
    10021 aataaaaaaa gctaaatcta aaaaagattc agctctggat gtacactaat tagcaaccaa
    10081 aataaaagta ttagtaaata ctgtttttgg tagataatcc ctgtcctttt gcctgagaga
    10141 ttgagcataa aagccttgcc ccttcggcgc ccataatggg tctctccaga gttccgtaaa
    10201 ttttacagtc ctacttttaa aagtacctga gagtgttact ccttcggtgt gctatatagc
    10261 aactcttcta taaaatctta accggatatt gatttgtcat agcttattgt attaataata
    10321 ttaatataag tttttatgtt ttgtcaacta aaaaattaaa aaacaaagaa aaaatataaa
    10381 aatgtaagcc tatttacgaa ttataaactt agaaagatga gtaatatacg tgaaaatgaa
    10441 ttttgtaata cacatacaaa tgaagtgtaa aataagtttt aaataaaaaa ataataaaat
    10501 cttaaataat taattagata tgattaaatt atggtaatat tatagttacg atattaagaa
    10561 aagttctatt tagttagatt ttaagcgtaa tatgagatta tgtatagaga aaggatgtat
    10621 ttttagatgg gaaaaatatt agtgctagca gaaaaacctt ctgttggaag agatatagca
    10681 aaagttttag ggtcaaaaaa tgaaaaaaat ggttatatag aggggccaaa gtatgtgata
    10741 acttgggcac taggtcattt ggttacactt gctgaccccg aaagttatgg agaaagatat
    10801 aaatcatgga gcttagaaga cctgccaata cttcccaagc accttaagac tgttgtaata
    10861 aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa tgaatagaaa tgatatagat
    10921 gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac ttgtagcaag gtggataata
    10981 gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga tttcttcaag tacagacaaa
    11041 gcaataaaag acggatttgc aaaattaaga agtggaaaag aatatgaaaa tttgtattac
    11101 tcagcaatag cacgagcaga agctgattgg attattggaa taaatgcaac cagagctttg
    11161 acaacaaagt acaatgcaca gctttcttgt ggaagagtac aaactcctac tcttgcaatg
    11221 ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta aagagtatta tggattagaa
    11281 ttaattgcta caaagggaaa ttcagatata aaatttattt ggaatgataa aaacaataat
    11341 tcttcaactt ttagtaaaga aaagatagaa tccacattaa aaaaagtaaa aggtgtagac
    11401 ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat attcacctgc actctatgat
    11461 ttaacagaat tacaaagaga tgcaaataaa atgtttggat tttcagcaaa agaaacttta
    11521 tctataatgc aaaaactgta tgaacatcat aaagtactta cttatcctag aactgattct
    11581 agatatttaa catcagatat tgttggaaca ttaaaagata gaataaaagc tgtaaatatt
    11641 agtgattatt caaagatttg taatagatta ttaaaaagca atattatagc taataaatcg
    11701 tttgttgaca attcaaaggt aacagaccac catgcaataa taccaacaga acagaaggta
    11761 ttcatgagtg attttagtga taaagagcga aaaatatata atcttgtagt aaaaagattt
    11821 ttagcagtac tatctcctcc ttttgaatat gagcagacaa ctataagtgc aactgtagaa
    11881 ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt taggttggaa agaaagttat
    11941 aaagatattg aagatgaaga tgagtatgaa agtataccaa atgttttaga aaaagatgta
    12001 cttaaggtca agggattaaa gataacttca ggaaaaacaa atccaccacc atacttaaat
    12061 gagggaacat tattgactgc tatggaaaag aatggtctag ggacagttgc tacaagagca
    12121 gatataatag aaaaattatt caattcattt ttaatggaaa agaaaaataa agatatatat
    12181 ataacatcaa aaggaagaca attattagat ttagttccag tagacttaaa gacaccagaa
    12241 cttacagctt catgggaaaa gaaattattg gatatttcaa agggaaaact tagtaaaaat
    12301 acattcataa atgacatgaa agattattca aaagatattg tactagaagt aaaaaatagt
    12361 gataataaat ttaaacatga taacttaact aaaaatagat gtccagaatg tggtaaattt
    12421 atgttagagg taaatggtaa aaaaggtaag atgttgattt gtgaagatag agagtgtaat
    12481 actagaaaaa ctatatcaca agtaaccaat tcaagatgtc ctaactgcca taaaaaatta
    12541 gaacttcgtg gagaaggtga ggggagactc ttcacttgta gttgcggata cagagaaaag
    12601 ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga aggcatcaaa aaaagatgtt
    12661 aatcaatatt taaaaaatca aaataaaaat caagagacat ttaataatcc atttgcagaa
    12721 gcattagcta agctaaaaaa caaataattt taaagttatt attatacaca attataactt
    12781 taactgtata ttagttatat gtactgatat aatatagtgt atagtttagg catgaaagta
    12841 gggctgttaa atggatttaa gtggcatatt taaatactat tgtaaagagt gtgaaaatac
    12901 atggaataat tcgagtgttg aattatttga gaatatagaa acgtatagta aagattcaca
    12961 aaaaaagagg gaaaaagaat tagataaatt gctaaataca atatcagttc atttagagag
    13021 gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa aagggcgagg ttttcttaca
    13081 aaagacattg gaaaaagaaa atatttttaa gttagaaaaa atggatgtag aggatagaaa
    13141 aaaattttta gatataacaa aacagtttat tagagatgct agaaaatttg atgatgattt
    13201 acctataggt gatattatgc aagctatgag gaatgtatgg atttcaaatg cattacaatt
    13261 attatttggt aaagaagtat attattcaaa agctaacttt gcatatagta tgttatatcc
    13321 atatactgat aattatttag acaatacaaa tatagataaa aatgataaga ttttatttaa
    13381 taactggtta gaaaaaaggc tcctgggaga acacattaaa tctaaggatt atcatgaaag
    13441 taaagtatct caaatgatag attatattga aagtgtatac cctagagaaa agtttacaga
    13501 agtttatgaa tcgttattat taatatttaa aagtcaagta aatagtttaa aacaacatgg
    13561 taaggaaaat catttgtgta aagaagattt attatccatt tctattgaaa aaggaggttc
    13621 atccgtttta gtagatggat atttaataag tggattgatg acaaaggaag aaatagagtt
    13681 ttgtatagga tatggatttt tattacaaat atctgatgat ttacaagata taaaagaaga
    13741 tttaaaatac aaccataaaa ctattattac agagatgtca aaagagggta ctttagataa
    13801 agttgtaaat aaactaataa attttactat tgagttaata gatagtttta aaattaataa
    13861 taaaaataaa tctgtaatag ctatgataaa gaatgattgc ttaatgttaa ttttattttc
    13921 tgtagtttat aatgctgaat ttttttctgt aggatatata aaagaagtag agaaatttat
    13981 tccatataca atagattatt cattagagat tgaagaaaaa ataaaagaaa aatttaaaaa
    14041 tatagatgtt ttaaataatg aaaatgaata taaagaaatg attgatatta tttgtgcaga
    14101 gtagaaaaaa agggagtgtc ttagaataga ctttttagtt tatgaggcac tctttttgta
    14161 taaaatcaaa tgtattttca tcattttaac aattaaaaag agcttatccc tatttgagaa
    14221 agcccttttt aatttaaata aaaatgtcaa ggttaaacta tttaaatgca gtaataatcc
    14281 cattatcatg taagtttttt atgattgtag ctgcgatagg gaatccaaat aatccagcta
    14341 ttccaaatag ttgagctcca acaaacatac acattaatgt tattaaagga tataggccaa
    14401 tttggtgtcc tactaccttt ggttctaata tttgtctaac aacggttata attatgtata
    14461 gtattaaaag tcctattgct aaattaatat ttccattaat gagtgagata atcatccaag
    14521 gtgtcaaaat acttcctgta cccaagactg gcaaaatatc aacaagtgct atgatgacag
    14581 atatagtaaa tgcattttca acctttagga tagtaagtcc aataagtagt tctatgaatg
    14641 tcactgatag tagtattgca taggctttta gtagttttaa aagagcatta atactgctat
    14701 tttttatatt tattatcata aattgacttt tttctggaaa ttgttttaag ataaagttta
    14761 ctattttttg atagtcaaaa gtaaagaaaa aagaagcaat taaagtaata ataagtttta
    14821 acataaatgc aggtgtttta cttgcaatac tagcaatagc attgagtgca tttgttgaaa
    14881 tagaagctac taaagacatc atagattgac ttatattatc ccaaccaagt attaatgcta
    14941 ggttaacatt aggtgtacta gcatctatct ttgaaaataa agtatttagc gttggttgta
    15001 tacttgattt atatatttca ggaagtctaa aaaatacatc acttacactt gcaaatatct
    15061 tcacaccaaa gctaactaat aaaaatatac acaacccata aaaaattaaa agtattacta
    15121 tagaaataag tgtacgattt aaatttgtat tatttgtaat aagtcttata ataggtctta
    15181 agatggatgc aatgataaaa ctgattacaa aaggcattaa taatggcaat aagaatttta
    15241 atacaatata tacaatactt gttattaaaa ctacatatgt aaatttaatt ataaaattct
    15301 tttgcttttg aatatccata atataaaacc tccaatgtat ttgatattat tctaaatatg
    15361 gtctttttta cagaaaatat actactgtat attgattata aactataaag cttatgtaaa
    15421 gtaaataaat tatgtaaaat tt
//
LOCUS       DAYAOY010000098        15363 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYAOY010000098 DAYAOY010000000
VERSION     DAYAOY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.098"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(405..587)
                     /locus_tag="ACGI6B_003128"
     CDS             complement(405..587)
                     /locus_tag="ACGI6B_003128"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752545.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
     regulatory      813..910
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            965..1141
                     /locus_tag="ACGI6B_003129"
     CDS             965..1141
                     /locus_tag="ACGI6B_003129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752546.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1367..1567
                     /locus_tag="ACGI6B_003130"
     CDS             1367..1567
                     /locus_tag="ACGI6B_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9752547.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2319..2501
                     /locus_tag="ACGI6B_003131"
     CDS             2319..2501
                     /locus_tag="ACGI6B_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFG9752548.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            2572..3417
                     /locus_tag="ACGI6B_003132"
     CDS             2572..3417
                     /locus_tag="ACGI6B_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9752549.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3641..3805)
                     /locus_tag="ACGI6B_003133"
     CDS             complement(3641..3805)
                     /locus_tag="ACGI6B_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752550.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(4347..4679)
                     /locus_tag="ACGI6B_003134"
     CDS             complement(4347..4679)
                     /locus_tag="ACGI6B_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFG9752551.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(4923..5564)
                     /locus_tag="ACGI6B_003135"
     CDS             complement(4923..5564)
                     /locus_tag="ACGI6B_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFG9752552.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(5545..5838)
                     /locus_tag="ACGI6B_003136"
     CDS             complement(5545..5838)
                     /locus_tag="ACGI6B_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HFG9752553.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            6219..6290
                     /locus_tag="ACGI6B_003137"
                     /pseudo
     CDS             6219..6290
                     /locus_tag="ACGI6B_003137"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6461..6595)
                     /locus_tag="ACGI6B_003138"
     CDS             complement(6461..6595)
                     /locus_tag="ACGI6B_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752554.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(6806..7558)
                     /locus_tag="ACGI6B_003139"
     CDS             complement(6806..7558)
                     /locus_tag="ACGI6B_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFG9752555.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(7617..9272)
                     /locus_tag="ACGI6B_003140"
     CDS             complement(7617..9272)
                     /locus_tag="ACGI6B_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752556.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(10545..11624)
                     /locus_tag="ACGI6B_003141"
     CDS             complement(10545..11624)
                     /locus_tag="ACGI6B_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9752557.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(11708..12364)
                     /locus_tag="ACGI6B_003142"
     CDS             complement(11708..12364)
                     /locus_tag="ACGI6B_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9752558.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            12686..12892
                     /locus_tag="ACGI6B_003143"
     CDS             12686..12892
                     /locus_tag="ACGI6B_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752559.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(13045..13473)
                     /locus_tag="ACGI6B_003144"
     CDS             complement(13045..13473)
                     /locus_tag="ACGI6B_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFG9752560.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(13949..14887)
                     /locus_tag="ACGI6B_003145"
     CDS             complement(13949..14887)
                     /locus_tag="ACGI6B_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFG9752561.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         5351 a         2464 c         1640 g         5908 t
ORIGIN      
        1 ttatctatag tgttacttat attttatata tatggctatt tttcaacatt ttacaatata
       61 taacatttat ttttttaact tttgtcagta aaatgtcagc aaaacacttt atccacaatt
      121 tttatataag ttttccacaa cttatcacca atattatcca cataaaaaag gtagcactta
      181 tattattagc taccttttat ttattaatct ttatatttcc agctttttat ccaacttcct
      241 atttctatta ttttttcttc agttatttct tctccatcat gtaattttga aattaattct
      301 gataaaggat tgttatgtaa acctctaaaa actctttttc cctctatttt gagatattct
      361 ctctctttta ttaatactgt atagtgtaca tgcctcccaa ttctctatga tattaaaaaa
      421 ccttttttgg ttaatctagc taaaagtata attgtagttg tatgcttcca accatatgtt
      481 ttttctattt catctgcaac atcttttgat attacagttt tataaccagt atgccatatg
      541 catttcataa tttttaattc tgtatctggt aattttttat ttagcataat atattccttc
      601 tttcaattta tgtcacttct tcaaatttat ctagcttaaa ttataatagg agctttttag
      661 aacaaaaact atatttaatt tttaccaggt actctacgtg tttagtctgt ttttaagtgt
      721 aaaataatgt tcatatctat aaatttattt aactttcaaa caatcctgtt gaataataac
      781 taattctatt gtattatttt agttgtagaa taaaactaaa tcggcaaaac tagagaaatt
      841 tagtgacgca aagctatagg gactaagact tatataaata tcttatgagt tatgtcagcc
      901 agttgccaaa gagatagtgt tctttttgtt tttatgaaag ttttttaggg ggataataat
      961 ttatatgttt ggaaataaaa tggataaatg tacacatgta ttgactgctt atattagcag
     1021 ttcgtatgat tattgtaatt ttttagatac acagttagat gattttatat tagagtacgg
     1081 agaaaatgta gtagagtctt gtttacacca agtaatggta ttggtaagta agtataatta
     1141 gatagacatt gatttctaga aaaccctttt tagttttatc tacatttata ttaatatata
     1201 gcaatgtaaa acgtttaaat atatttttaa tattaagaat aattaaaatg ttaactaaaa
     1261 aatataatta agtagacatt tctattaatc tatgatacaa tagtatttgt ggtaatacac
     1321 aattaaatca agtatatctt gatataggtt aataggagta tttttaatga ataatggaat
     1381 agtgaaatgg tttaataatg aaaaaggatt cggttttata tcaatggaag gtagagatga
     1441 tgtgtttgct catttctcag ctatacaaac ttcaggattt aaatcattag aagaaggtca
     1501 acaagtaagc tttgatatag ttaaaggtgc tagaggtcct caagcagaaa atataactat
     1561 attataatat atagattttt cgggaaagac tctttttaga gtcttttttt attgttaata
     1621 tatgtaagat aatcattggt gaagtaaata aggtatattg agtaatgttc ttgatagttt
     1681 atatgctaat gaatttcaaa aaaataagca ctcctataaa aagagtgctc ttaatgtaaa
     1741 tataaacctt cattggtcca gcctaatacc ccgtattctc caagttctaa ctatttttta
     1801 tatacattat cactttcatc catatctaac acaattattt cttctttaga atcaatttgt
     1861 aaaatatgag gactgtgtgt tgtgacaatt atttgtgttt ttaggagaat tgcttttaat
     1921 gtaataaaaa ttttacatac cacatagttg atataaaact ataataatta cataagattg
     1981 taataataag acttatcttt acataccaca tagttgatat aaaacaatga aattaacata
     2041 caagagacta agacaattaa aatctttaca taccacatag ttgatataaa accccaaaat
     2101 aaacttagca tctccaatgt ttacacatat acaatgctct taaaattgca gtgaacgcgc
     2161 aatagtgcaa ttgataatac ttattatata ttctcaatgc cttatatttc aactgctaaa
     2221 cctcaatttg tcacaaatat cgctcactgc aaaatttcta tatttttatt atatcataac
     2281 atataattaa tattttcaat taatttttac atttaattat gtttaaattc tataataatg
     2341 caactttaat gtttctttct ggaactaata taaaaactat ttcagaaaga ttaggtcatg
     2401 cagatataaa aataactatg aatagctact ctcagttttt agaagagatg gacagagaag
     2461 catttgaaaa tttgagcaaa atactattta ttatatttta aagaagatga cttattcaat
     2521 atttgatgct agaatataat taataaaaat ttaattatag gtgattgtat tatgaatatt
     2581 attaaaagca taactttact cttaacatta ttaattgtga tattaatgac aggctgttcc
     2641 ccaagagaaa gtgctagtga agttgcaaaa aaatatattg ataagatgaa ctctggtgac
     2701 tatgaaacag catatagttt attaagtaaa gatagtaaaa aaaaaattag tatagaaaat
     2761 cttgaagaat ttcaaaatat tttagttagt acaaaaagaa taattagtta taaaataggt
     2821 aaagaaaaaa tcataaaaaa atataaacat gatggaaaag aatataaaaa tgttgttaaa
     2881 ttagaagaaa cttttaatat aaaaaacctt ctttataaaa aagatgattc tctaaaagta
     2941 aatagatatt ttgtagttga aaataacaaa tacaagctat tattgtatga aagtttcaaa
     3001 aaagaatttg gtattaatag tatggacctt gctcaactta agcttaatga ttttggacta
     3061 aaatacttta aagaagttga ccttatacta aaaaaagcta ttgctattaa tcctactgat
     3121 tcaaagcttt attatgccca agcatgtaat tatatgaatt ttgatggatt tcctggttat
     3181 tcagatacaa catataatgc tttagattca attaataaat ctttaaagta tttagataaa
     3241 aatgattctg actacaaaaa aaatgcaagt aatatatata acttaaaagg agttattctt
     3301 atgtttcaca aaagatttga tgaagcacaa caatatttaa ataaagcctt atactttaac
     3361 aaagataatg aagacccaaa atataatcta aatacaataa aaaaacatct aaactagata
     3421 tttaattttt gaatttataa aatatttttt tatgtataat tcagcactga atcataattt
     3481 ctactaaact aattacaagt ttaaattata aagacttatt tctatataaa tattactcta
     3541 taacaataag tttcttattt aaattttaaa atattatcta aaaatcataa aaaagaaggt
     3601 ataaagagct accctcaagc tcaatatacc ttataaatat ctaaacttca ttttttacta
     3661 atttttctat atctttgata atatgttctt tagatttttt tccagctatg gcatcaaaaa
     3721 ttgcaacagc ctttttctct ccaaactttt ctgctacatc attaaagtct aaaaatctaa
     3781 cactttcttt tggtttacca aacatataac acctctcata catttttata tatttattat
     3841 aacttctata gacttatttt tcaccaataa acgttagaca aattagtccc tttatttttt
     3901 taaactagct ttaccttcaa aattcttaac aaaaacagtt tattctactc tataaataaa
     3961 aatagacaat ttaatttgta ggaaactgat aatagaatac agtaaataaa actttgttta
     4021 atctatctga aaaagttatc tattaaaact gctatgaatt tataaagaat attctagttc
     4081 ataaaaatac aaatatgtaa atataatcac cttaatattc aatttattta aaataaaaaa
     4141 tgtataaaag atttaataat gacaatatac tattctcaat acaatctatg atgtaaataa
     4201 tttaaaacta ctatattatt taaaaattta tatttatata tgcaattcta aaaacttaat
     4261 aagaagtgaa cttagaaaat aaagcttaat ttcaaagact taaatagact attattatta
     4321 gtctaaataa actacgtcta tcaaaattat atattaaatt cttctaaaat ttttattgca
     4381 aatcttgcgt ttggatatgt aatagacccg ccaccaatta cacctatttt tatagcttct
     4441 ataatttcat ctttacttgc tccagaatta atacaaccct caatatgata acaaatacac
     4501 tcatcacatc ggcttaaaat tgaaatagct agtcccatta attccttatt ttttttgctt
     4561 atagcccctt ctgaataagt cttatcatca agacttccaa atactctaaa aaactcatct
     4621 gtttcgctta attttttatt gtaaaatccc ctatattctt tcatttctct agtattcatc
     4681 ttctacctcc tatgtttttc ttataagaat atcacaccgt acttatattt tcaatattaa
     4741 atacaaattt tcattttatg ttcattagcc taatttatat tattttttga aatctcaata
     4801 agttatcata taagtaagat tttttcagaa ctaaaataga aatcttagta atttctttat
     4861 tatctatctc agttctgaaa tatattattc ttatccaaac attattatag tgattaatta
     4921 tattagttat ttttacctga aattttatac atcaaataag aatatataaa tggtagtatt
     4981 acaattacaa agattacaaa gatattataa aaaggaatat taataaatgc attaataatt
     5041 aataatattc caccaataag ccaaatccat ccagctaaat ggtgtgtctt attccaatta
     5101 tcttcatcat taagagtcca tggtaaaatt attcctactg agtaatttcg tttacattta
     5161 ggaagatagt ttccaaataa aataactatt attcctaaaa ttaatcctgt ataaaaatta
     5221 gactccaaac taactcctgt tgaatataaa accatcatta tttgtataat tactgaaagt
     5281 atagcaactc cccattttga taaattttgt ataacatttg aaaaattagc tttcttaggc
     5341 tcattctcaa gtctaaacca tgtatacaaa tggatacata gcattaacaa tggtacacca
     5401 aaagctgcta taacctttgg agcataatta tctggatttc cataattatc aaaatgaatt
     5461 gcaacttcat ttggtaatct attataaaaa tatattgata caataaatgg tataaaacat
     5521 attaaagtag ataaaataaa ttgtttattt atcgcttttt tcattatctt tttctccctt
     5581 ctccataaat tgtgaaaacc aaagcatgac ctcttcgaaa actgaaatat ttatttcgta
     5641 gaaaataaag ttcttatact ttgtttcaaa tatgagtcct gcctttttta gttgagatag
     5701 atgataagaa attgtagcat tcgtcatatt aaatttttca ctaatttctc ctgctgacat
     5761 tttattctcc ctcaacataa ccaatatttc tcttcttata gggtctgaaa gagctttgaa
     5821 tgtttctgga aaccccaaca ccatcacctc gctatttaga atttcatcta aatagaatat
     5881 aacataacat ccattttatt tcaatagcta tttagaaatt tttctaaata gttatttcta
     5941 aatatttatt gaaaattttt atattaagat tctcaattta cttaagtcgc ttttatttaa
     6001 tcttacttta tctaattttt ttaacttaac aaaagtgtat tgcaccataa ttgttaaagc
     6061 aaaaacctaa tgattgaggt acttatatat ataacaatac ttttaatttt aaattaaaag
     6121 tagctaaacc caatttaaaa agtagttaag tcctatttaa atagaaaaga atgaaacgag
     6181 aaatatactg tataaaagta cataaaaaca tctactaatt agatacaact ataacagaat
     6241 atatttatta ttataataac aaaagaataa aaaaaattaa tttagcataa ccttgttaaa
     6301 tatagatttg aatatgaatt taaagttatg taaaattaaa aatacaggaa tgaaaattcc
     6361 attcctgtat aaaatctaac ttttgagtct cagtacaatc ttattttgag acaatctttt
     6421 ttgttttata ttttcttata tttaaaatat aacgcatatc ttatgcaaca tattcatatt
     6481 tttctttata ttctttcatc gcatcattcc aagcgtcttg tactgttaca cctcttttat
     6541 tagcaatttt ttgtgccagt tcaattatct tcatagctct tcttgatatg ttcataaaca
     6601 ttcctcctac aaattaccat atatttcata tatataaatc aaggttatat ttttatgata
     6661 tataatcctt ctattgtaag attacagttt atatgtgtat tatatatgaa ctatattaga
     6721 agataattag aatttttata aagctatatg attagattaa atataaaagc tttacaatta
     6781 cttataaatt taaaaagtct ataagctaaa ctaatgtttg atataccaat tttacgacca
     6841 taaataatgc cattactaaa aatattggtt ttatcatctt tgaacctttc tttatagcaa
     6901 ctttagcacc tacataagct cccattatca tcactaatgc aaacactata gcaattttat
     6961 aatctacttg accatttatc atgaataata acaatgaagt tatattactt gttaagttta
     7021 atatttttgt atttgcagaa gcatgtaaaa aatcatatcc atatattttt ataaatccaa
     7081 aagtcaaaaa agttcctgta cctggaccaa aaaaaccatc ataaaaaccc attataagag
     7141 ccataagcat ccctaatctt aaatttttct tattaacagc ttcaaaatta tcttcttgac
     7201 ctaaattttt agaaataaag gtgtaaatag ccactattaa aatcataacc acaaccaaaa
     7261 catttaatat ctcttcactt attgataata cacatctcac acctagaaca ctaccaataa
     7321 tagtaaatgg tactaatttt tttaaaaggt tgttattaat ttttccagac tttgcatatc
     7381 tataggcact acttatacat cctgctgatg ctgcaaattt atttgtacca atggcaagat
     7441 gtactggcac tccaattgcc ataagaacag gcatgcttat aagtcctcct cctcctgcta
     7501 tagaatctac aaaagcagct aaaaaccccc ctatacataa aaaaataact gaaaacataa
     7561 aattcctcca tcattctctt tcaatttttc ttacaatata tattatataa tcttatttaa
     7621 tacttattat ccagcatatt ctttctatat tcatttggag ttatactatt aaacttctta
     7681 aatatttgtg aaaaataact actgttatta aaacctactt tcttacaaat atcactaata
     7741 ctcatgcttc tatctaacag atatttttta gattcttcta tcctaagaag atttatatac
     7801 tcagttattg aaactttatt tctttcatta aaaatagaac tcaaataatt tggacttata
     7861 aaaaaaacat ctgatatact attcaaattt atattttctt tataatattt ttttatatat
     7921 tcgtttatat tttcaattag atttccttta tcttttctcc aaaatgctct attcttatca
     7981 atacactctt tggaaaattc aaataataca ttccataaat cttcatcact gtttacaagt
     8041 gatatttttt tattatattt atttcttaaa taagatataa gttttaaatc ctctaaatct
     8101 ctaagcactg ttctagacaa tacaaatatc atttcctgaa tagtatctct aacatcatta
     8161 tatcttaatt tgtttgtatc tattagatta taaacacctt ctaaattcat atttttaatt
     8221 tctttttcaa tataaagttc taattcaaag ggatattctt ttgaagaatt attgaacata
     8281 tctatcggat taataatata ctcttcttct aataatctac tatttgcttt tttaagtgcc
     8341 tcttcaagtc ttatattttc ttttctactc ttcaatatct cctcatcttt tatactaagt
     8401 tgattttcaa aaaaattcct cttagctatt tcctgaataa attcagacat cattaataaa
     8461 ttttctttta atacatataa tctactttta ggatttaatg gaaattcatt gtaaattttg
     8521 tttatgtatt ttatatcaat accagaacta cttgataatt cagataaatt tttatctata
     8581 tcaatagact tatatactat aaaattacca cattctatat atccaactat attttcttct
     8641 aatactatag gtatctctaa actggaaaca ccatactcac aatggtacac tttttcaaag
     8701 ttatcattat ttatgctttt aacaagtttt ttagtacact gttttatatt tttacaaata
     8761 tcgcaaaatt tattttcttt cttagtctca aatatttctt tatactttaa gtcatataca
     8821 gatattctaa gcttcaatat attagatatt tgatttgcaa gattttcaat tatgtttaca
     8881 ttaactaact cctgtatatt gttttcagaa aatactatag gtatttgtct caaactcgaa
     8941 aatttatgag gaacataagt aattaatatt actaaatcaa ttttaccagt gtttataatt
     9001 tcatgttctg aatatggtgg gcaataaact atagatgttt cctttatatt gaccttttta
     9061 ccatcaatta tttgtattcc ttctccctga atcacatata aaagctgttc ttcacttaaa
     9121 tgaaaatgct tttcttggta gtttcccgga aaaaatctta caattccagc actaagtcta
     9181 ttgaattgtg tttttgatgg ttcgtgtatc cacataacct ctccccaatc aaatttttgt
     9241 acttcattat ttgactcact aaaatactcc ataagctcac ctcttagttt ttgtctaaat
     9301 attttttctt cccttcttat attaaaataa tatcatcatc attaactttc attactataa
     9361 aatcatcata ctcttttaat actcctgtaa ttttagttgc acttagttta ctacaattca
     9421 gttttttaat atattgataa attctataat tactttttta ttttgtaaaa tcagtgtttt
     9481 cccaaaataa ttatatctat aaatgaaagt atttttactg cttacatcaa tatatgttat
     9541 cataattttc ctttttattg catatataaa taactgtata taacattgtc caatactata
     9601 tagaaataca tctaagaaag aatattatta aattgttcca attagtatta tttttcttat
     9661 agaaataaaa aaactgttgg agtcttttag aaaaattatt ttttacatac cttttttgct
     9721 tagatagtct atcttcacag ctattttgta tatacactct tctagttcat gattttattt
     9781 taatatcttg cttatattgc tgaaataatt gaattctttt atctatcata ttgtctctta
     9841 tatcacaaac atattgtttc ttatatcaca aaaatgaaag cattcttata tcttcaatat
     9901 ataaacctgc tttttttaat cttgtaataa atataggata ctctatattc tcttcctaga
     9961 aataactaat accacttatt tttctattat atagaaagta ataaatcttt ttcttaataa
    10021 aattcaatag taagtggaat aaaattttat atgaataaga acattataaa aatactttat
    10081 acaatagtca ataaaagata ttagcttcaa attatgttaa agttgtatta tttaaagtga
    10141 aaaaacaaat aaaaaagaaa taatagtata agtagaaaaa tatacttaaa acaaggcgaa
    10201 gcctctcttg tatctaaatc aagtagagaa ttaaatctaa aaaagaaaat aaatataaaa
    10261 gatgactcct gtacattaca agaatcatct tatggtttca taatcactcc atggttttta
    10321 actgtctgat ataaaggtct cagttcactc atagcatgac caccttttcc gattgctgac
    10381 cccgtcactt atggacaaaa tgtccataag tgatagtcct ctcttaaaac cctctaaata
    10441 gatatatctc aagcaccgca ttgaaaccag ttgctggaaa caaactgaag cgttctggaa
    10501 tagtaaaaga aattgccacc atatccacgt tactgtccta gttgttactt tgctaacttc
    10561 ggagcgattt cctctaacaa tacgcagtca aattctgcct ttttgctaag aaatggcaga
    10621 tggtctgcat ctggtataaa aaaaatctgt ttgtctggcg ccactatttc atcaaaaaat
    10681 ttcttcgctg gaagataggg gctattatag tcattttctc ccatgatgta gtatacaggg
    10741 acctgatagt caactccata atttcttatg tcagcttctt ccactaaaaa ccgcaataca
    10801 tcaccttgat tgtcaatcgc atttggattg aaaatggccg tattttttaa ctctctcaat
    10861 gaataatacg gggttgccag tatactgatt atattgccca aatccatatt tatagaaagc
    10921 ccatacttgc tttggtatgt tcttactgtc ataagagctg ccaaatagct tggaccatat
    10981 tttgctggcg gatatggtgc aatagcttcc agcgccttga tatctttttg cttccctgca
    11041 gctttcacca tttcaagtgt cttttcatag cccacgcgct cattgtccac catattaacg
    11101 gtctgcgcaa tcccaatgta cgcgctgtaa tcctccggat aggtttgcac agccatagtt
    11161 cccagaactg ttccccagct atgacctata aggataattt tctccttgtt gaactcttgc
    11221 ttcaaatatg ctgtcacctc atggacgtct tgcagaacac gctccgcgct catggtctgc
    11281 aaaactgctg ccggatcgtt cgcaaagtat gttttgccta cgtttcgctg atcccagttc
    11341 accacagtaa aatcattttc ccactcatac tggtacagat gtaaaaatgg catcattgaa
    11401 ttaccaggac ccccatgaac ccaaaggatg atggggttgt ctgcgttttg tccccggata
    11461 tataacgcct gctgtatgcc gccgatctcc accatttcca tgcggtcgat tccaggagct
    11521 tctattttat ggcttgtacg tataatcgtg ggggctatca gacgcaaacc ggcaaacgcc
    11581 agaacaaaaa cagcaactag tatgcaaagt gtgattaata tcattttaag taccttcttt
    11641 catgaatttt tttagccata gatatctcct ctaacattaa aaaaaatatg ttgctatttt
    11701 agttttgtta ctctacctgt cccattggtt tcctttcata aaaaatatta attgattcta
    11761 taatctcatc ttccaggata tcaactccta tttgtctcat aagaaactgt aagaattgaa
    11821 accgtttacg aagtgtagga aagtccccct gaaatacatc tgtatcacac caaaagttaa
    11881 agagcagcaa aattacctct gcgcattcat ctgggaactc tgtgatgatg cttctatcct
    11941 ctatgccttc tcgtaaaaca tttgctaata ttggtgctag ttttttcaaa ttatcctgtg
    12001 taaatgccag tatgatatgc ggactttcta tcgcgcttgt aatcatgtta ctagattctt
    12061 ttatcacctc atccgttaaa tgccgcctaa ctaaacttct aagcttctct tttgctgtaa
    12121 gtccatgcat ttcaccgagc cattgattta ctgtctctat tatctgttca aagcgccttt
    12181 ccatgaccac attaaaaata tcttcttttg aactgaagtg atgaaaaatt ccgccctttg
    12241 acattcctga agcatcaaca atattttgca tacttgtttt gtcatatcct ttttcagcaa
    12301 acaactttgc agaaatggta atgatatttt caatagcttg ctttggattg ttctttctag
    12361 tcattatata actccttctt attaaaaacc gactgttggt ttttattata taagctattc
    12421 ctgcttctgt caagtaacat tgtgtttatg gcaaaacaat aaggtttggt ggttagacag
    12481 gtattaatta gactaatgcg actttcccct agatggaacc cttggtaaat ttgcaatctt
    12541 taccggcttt gattatatct gtaagcaagg caggtatggc cacacctgcc ctgtttgccc
    12601 ctcggatatt cattcctatc cctcctctcc ttagccatta caccaaaacg ccgctcattg
    12661 tctccaatgg cggcgtttta aagatatgat ttctcatgct gctgaaatat gcaaaaaata
    12721 taagatgccc tttattattt ttcctacaat agaaaagaaa agtattgaag aatgggttaa
    12781 tattggagca aatatgcttt gtttggcaca agatactacc tttatcactt atgttatcaa
    12841 tcaattatta tctttttata atgagattgt accaattcaa aaacaacatt aaattataaa
    12901 tctaatttta tattaaaaga gactaatatt ttatctatct ctattaagga tattttctta
    12961 tgtatcataa tattagtctc tcttattatt atatactgct atcatcatta gttgctattt
    13021 atactatttt tataatatac aaatttattc agctaacgtt ttgcctaatt ctatacactc
    13081 atcttttgct tcttcatttg ggtcttccat tgctgtaatt ccttctccac caataattgt
    13141 agcaccagca ttttgcatac gttcttccca gtctctcatc cattcacaat ttccccatcc
    13201 atatgaacca aataatccaa tctgtttacc tgataccata gattctaatt ctgaaacaaa
    13261 tggctccata tacccctctt ctagttgctc tgctcccata gatgggcaac ctaatgcaaa
    13321 tttatcttct tcttttaaat cacttggttt cagtaagctc acatctaaaa cttctggtgt
    13381 cttacctttt aacttcacac cttcagccac aaaatttgcc attttttcag tatttcctgt
    13441 tccactccaa taaacaatat atattttact catttttacc tcctgtagta ttttttaatt
    13501 aatcaatagt tagttatagc taactaacat ctaaaaaaca aaagatttct ttatctgaaa
    13561 tctctttatt tttctaaata tagaatatca tggttgattt tgaaattcaa tacttattga
    13621 taatatttat catttatatt ttatattcat tttataatat caaaacactg tatcgtattt
    13681 ctaatctttc atagaaaaat ttctaactta tagtactttt gcatctctta taaatctttt
    13741 atatgtataa gtacatagaa aaacacacct tttccagatg tgctatctat aatatcgtat
    13801 atttattttt caattctaac tagctaatat gtttataaat tttatcactt aataccttta
    13861 tacattgatg taaaatctta cacctaagta atttttatta tttgtctcga ctctatataa
    13921 tatagctatt ttatcatcaa atcaaaaatt atcttttgct attattcttg tatttataaa
    13981 gatttaaata taagtatcca tttccttgtt ccgttctagg tattccaagc gctctcgtac
    14041 attttattaa ttgtgcatac agttcagctt catctaaatc tctttcaaac tcctctcttg
    14101 cccattcttt aattagtgct aatgagcctg atacgtatgg tgcactcata ctggtaccac
    14161 tcataatagc aagtttatta tccatataag tcgatataat atttcttcct ggagctacca
    14221 aatctattgt agtatttgaa ttactaaact tttcaaccaa atagttctca tttattgcac
    14281 ctacttctat tacctcagca taactggctg gataactata ctcacttgta ctagaatcac
    14341 catctccatt attacctgct gcacacacta cagaaatatt attttttact gcttgcatga
    14401 cagcattttt taaattctta tcatctttgt ttcccccaag agacatagat ataatatcaa
    14461 ccttgttatt tacagcaaag ttaatagcat taattatact ttgatatgtt ccagtaccat
    14521 ctttatttaa tgcttttgct attaataatt tacaatctgg agctactccc ataacttcat
    14581 tattataatt agatgcagct ataatacccg ccacatgagt tccatgacca ttaaaatcct
    14641 catatatatt tttatttcca ttactgtcat cactaaaatt tgcaccacca attatttttc
    14701 cttttaaaag aggatgagat atatcacaac ctgtatctat tataccaact acaatatttt
    14761 taccagtata accttcatcc cacattcccc tagcattcat ttgttttatt ccatatggga
    14821 atttattttt tgcacctctt agattttcat ttatttcata tggaattagt ttcattcttt
    14881 tattcatcat atatcctcct aaaaatttta ttgcttataa aaatcatgaa ttttatttta
    14941 taataggttt ataaatcaaa ttaaaaccta tatctactta tttatattta tgatatattt
    15001 tttattttta ggagtcatat tttattgtat ataaaataat atttagataa ctatattaaa
    15061 aaatacatgt ttgcttatat acaaaactta ctaaaatgtc tttaatattt ttgtatacaa
    15121 acattatatt ttttatataa aaagctcata aaaattttga ttatattttc ataagttaac
    15181 aaatgtgggt ataatatgaa tttacaaaat tcctctgttt gtataagtgc tttaccaata
    15241 caacaatata taatcacatt gaagctatat ttgtatttat agtagataat gtatcaaaaa
    15301 ttggttatga atagtaaaaa taccagttaa gcatattttc atgtttctat ttttaacatt
    15361 aaa
//
LOCUS       DAYAOY010000099        15292 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYAOY010000099 DAYAOY010000000
VERSION     DAYAOY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.099"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..404
                     /locus_tag="ACGI6B_003146"
     CDS             <1..404
                     /locus_tag="ACGI6B_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752562.1"
                     /translation="NRKGEVKKTICIKYKNGVIRATNGDFIIKDSEGKIYSCTFSEFE
                     KLYENLERGHRYVDEDNNKKNKLELSAKLELDTTDFEENIKSATKEIETLNEVVDRLE
                     KKLNRIFAKENKVDIDSIVKQLEERLRESIE"
     gene            423..695
                     /locus_tag="ACGI6B_003147"
     CDS             423..695
                     /locus_tag="ACGI6B_003147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752563.1"
                     /translation="MFKKKYIKKPSKISVRNIMAFIITVVGIALGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYGLAGVISGYLIGKAIYLIASLISHIIYE"
     gene            746..973
                     /locus_tag="ACGI6B_003148"
     CDS             746..973
                     /locus_tag="ACGI6B_003148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752564.1"
                     /translation="MKEYVIWFKSGNCVSGITNEDVTDKLMKDFMEADSDCRYLKGYL
                     DEDGTTVIDLSQIEAISINNCSENNIGFSKS"
     gene            1019..1591
                     /locus_tag="ACGI6B_003149"
     CDS             1019..1591
                     /locus_tag="ACGI6B_003149"
                     /inference="COORDINATES: protein motif:HMM:NF018515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9752565.1"
                     /translation="MKKGELIALGLSEEDAKKVEAESLKELENYINKIEYEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQKKLKEKEQKEFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDTWLTQKSFLIKTGETKINYSPDNGN
                     KNTLSRAENIAKEKNEEGSKNPYADAWSIK"
     gene            1607..1987
                     /locus_tag="ACGI6B_003150"
     CDS             1607..1987
                     /locus_tag="ACGI6B_003150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752566.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTALDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSSGSYTYTLSEDPVGIVFSTVDVTYGPQPVASMVEGYVIIE
                     RLQGEYVKEAIETIKTKLPNIKFM"
     gene            1999..3018
                     /locus_tag="ACGI6B_003151"
     CDS             1999..3018
                     /locus_tag="ACGI6B_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFG9752567.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEEPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLSTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTSKTILNIILRDEKLRKAIFGVNS
                     DKLLTLNELNTFLVSQSLPPIFTYDERYRVQGKDGKYTTKRFLDENKFILMPDGKMGD
                     TFFGLTAEELELRKNPAIDISSVGNIIVEQYSTADPVAKWIKAVATALPSFPYADQVF
                     MGTIN"
     gene            3029..3373
                     /locus_tag="ACGI6B_003152"
     CDS             3029..3373
                     /locus_tag="ACGI6B_003152"
                     /inference="COORDINATES: protein motif:HMM:NF016990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9752568.1"
                     /translation="MEVERLKKLLGISKEDYSKEMILEFILEDVEEIVKNYCNVSVIP
                     EGLNSTVLRMAIDMYKNENLGSEDIALGSISSISEGDTSVSYRSSASEFKESLLKDYK
                     SQLNRYRKIRWK"
     gene            3376..3735
                     /locus_tag="ACGI6B_003153"
     CDS             3376..3735
                     /locus_tag="ACGI6B_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752569.1"
                     /translation="MDKTRKAIEMLYRDKCTIIEYQPVKDPVTKRTNNKEVIVLENQP
                     CKLSYKNINSTEEGKVAKLAQTIKLFISPNISVKAGSKLIITTQNNITREYVRSGESA
                     IYPNHQEIILELFKDKA"
     gene            3735..4160
                     /locus_tag="ACGI6B_003154"
     CDS             3735..4160
                     /locus_tag="ACGI6B_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9752570.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIERKLLILLKGVFDA"
     gene            4153..4590
                     /locus_tag="ACGI6B_003155"
     CDS             4153..4590
                     /locus_tag="ACGI6B_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9752571.1"
                     /translation="MLNNIIDGISIKLDKSFGNEYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPRSKDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTEKMNDVELFGGLKRGDNFE"
     gene            4574..4759
                     /locus_tag="ACGI6B_003156"
     CDS             4574..4759
                     /locus_tag="ACGI6B_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752572.1"
                     /translation="MIILNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELY
                     SFSEVEEIINNFMKGVS"
     gene            4760..6070
                     /locus_tag="ACGI6B_003157"
     CDS             4760..6070
                     /locus_tag="ACGI6B_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9752573.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEIFTVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATAKYSGTRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDTTLEASAGLVFTGGTNGESVTGAEYQAFLDKIESYSFNALGSLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKVKDTGLLESSLVYWTTGAIAVC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            6091..6567
                     /locus_tag="ACGI6B_003158"
     CDS             6091..6567
                     /locus_tag="ACGI6B_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9752574.1"
                     /translation="MAKNITMKSKDAISASLAECYVTIEGKRYLLMQCIKVEAKFKKN
                     KSKIPILGQTGKGNKTTGWEGTGSATVHYNTSIFRKLMLRYKETGEDIYFDMQITNED
                     PTSSVGRQTIILKDCNMDEVTLAKFDADSEYLDEDIDFTFDDWDMPEEFKILLGMI"
     gene            6636..7076
                     /locus_tag="ACGI6B_003159"
     CDS             6636..7076
                     /locus_tag="ACGI6B_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9752575.1"
                     /translation="MSNLSAFLSQNAIKNENVMYVASDRFLDEGGKPVEWELRVLSSE
                     EDEVLRRNCTKRVKVIGNNGKPTGQFTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTTFEDKVEEAKN"
     gene            7539..8099
                     /locus_tag="ACGI6B_003160"
     CDS             7539..8099
                     /locus_tag="ACGI6B_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HFG9752576.1"
                     /translation="MFCSNCGAEITGIGKFCSNCGAAVENEIIEDNNIKSNDLIIDAN
                     GIEVNMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDSSFEELKNNFIDDTMSNS
                     EKEKITNNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAVVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            8177..10543
                     /locus_tag="ACGI6B_003161"
     CDS             8177..10543
                     /locus_tag="ACGI6B_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFG9752577.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADEQQKRFNDDVRKGASSTDKLVENAKRLVA
                     TYIGLRSVGGLINLSDQMTSTNARLAMINDGQQSDGGLNKMIFQSAERARASYLDTAK
                     IVSRVGMNAGKAFSSTKEIVAFAEQLNKKFVIAGATTEETNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLEVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNSEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQAMSSRGFRTFIDNFIDSLYVLGAVAFSI
                     FNGIINILGSPFFQAFVNAIIVGVSLIVQVLGWVITQALNITNVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKTLAMNFAHIIAESYRAAALVASTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAVINKFAGTSYSAIGIVAGALSGLTAFIINSV
                     FFWINVFISFAEFFTNVLDHPVYSVKKLFFNLATAVLNNLISMTKGCDEFATNLANSI
                     IDGINGALKAWNKFVDVLNKFGGLGDKLGLGKADMVGHTKSITSTLQKAKGDLNKWLG
                     AEPKGYKSFKQLEYNNVGDWAKNGYAFGQNLENKIKDTFDISKIADKAKKDLGLDDLW
                     DDKYGLGDGIGSAGLNSPLGDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQET
                     INRFTGVNIKIDMNNTNNINKDADLDGIVNVLTEKLNDAMVVSAEGVV"
     gene            10559..11254
                     /locus_tag="ACGI6B_003162"
     CDS             10559..11254
                     /locus_tag="ACGI6B_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9752578.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKITNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFKDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPSVKIEPK
                     RVDSVNATNTKTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLS
                     KYTIYKGQVLKLG"
     gene            11247..13217
                     /locus_tag="ACGI6B_003163"
     CDS             11247..13217
                     /locus_tag="ACGI6B_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9752579.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIIAYDQLRYFKNK
                     DTYVYSNKTASELIKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMFTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            13231..13491
                     /locus_tag="ACGI6B_003164"
     CDS             13231..13491
                     /locus_tag="ACGI6B_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9752580.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            13496..13915
                     /locus_tag="ACGI6B_003165"
     CDS             13496..13915
                     /locus_tag="ACGI6B_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9752581.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVHSKYGNIKAEKVVSV"
     gene            13916..14965
                     /locus_tag="ACGI6B_003166"
     CDS             13916..14965
                     /locus_tag="ACGI6B_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9752582.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLSATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            14958..>15292
                     /locus_tag="ACGI6B_003167"
     CDS             14958..>15292
                     /locus_tag="ACGI6B_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9752583.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELT"
BASE COUNT         6158 a         1650 c         3016 g         4468 t
ORIGIN      
        1 tgaatcgtaa aggtgaagta aagaaaacta tctgtataaa atataaaaat ggagttatta
       61 gggcaactaa tggagatttt attataaaag atagtgaagg caagatttat tcatgtacat
      121 ttagtgaatt tgagaagtta tatgaaaatt tagaaagagg acatagatat gttgatgaag
      181 ataacaacaa gaaaaataaa ttagaacttt cagctaaatt ggaactagat acaactgatt
      241 ttgaggaaaa tataaaaagt gctacaaaag aaattgaaac actcaatgaa gtagtagata
      301 gattagagaa aaaattaaat agaatatttg caaaagaaaa taaagttgat atagatagta
      361 ttgtaaaaca attagaggaa cgtctaagag aaagtattga ataagttttg agggatggaa
      421 atatgtttaa aaagaaatat attaaaaagc caagcaaaat aagtgttaga aatattatgg
      481 catttattat tacagttgtt ggtatagctt taggtgtttt tataggtatc aacataatca
      541 tggctcatgt tttaggtata gctaacatgg tagataataa tacttttaca tgtgttagat
      601 tagtttatgg cttagcagga gttataagtg ggtatttgat aggaaaagca atatacctta
      661 tagcttcgct aataagccat attatttatg aataatttgt tcaaagattt tttattttgt
      721 aagaaatgaa aggagggttg tattattgaa agagtatgta atttggttta agagtggaaa
      781 ttgtgtatct ggaataacaa acgaagatgt tactgataag ttaatgaaag attttatgga
      841 agctgactca gattgtaggt atttgaaagg gtatttagat gaagatggaa caactgtaat
      901 agatttatca caaatagaag ctatatcaat aaataattgt agtgagaata atattggttt
      961 tagtaagtcc taacaagggc ttttttattt tctaaaaatg aaaggagaat taaataaaat
     1021 gaaaaaaggt gaactaattg cactaggtct tagtgaagaa gatgcaaaga aagtagaagc
     1081 tgaatcatta aaagaattag aaaattatat taataaaatt gagtatgaaa aggtaaaaga
     1141 ggaactaaaa gcctctaaag aagcaattga aggttttaag gatggaatga caaaagagca
     1201 gattgaagag cttaaaaaag gctatgagac taaattaact gcaaaagatg aagaatatca
     1261 aaagaaatta aaggaaaaag aacaaaaaga gtttgatatg gcgttagaaa atgaacttat
     1321 taaacttaat gttcatagca ctaaagcagc aaaagcagaa cttgatttag aaaaaataaa
     1381 atatgaaaat ggtactttta caggactaaa ggaacagact gatacttggt taactcaaaa
     1441 atctttctta ataaaaacag gagagactaa gataaattac agccccgata atggcaataa
     1501 aaatacatta agtagagctg aaaatattgc taaagaaaag aatgaggaag gttctaaaaa
     1561 tccatatgct gacgcatgga gtataaaata aaaaggagga taaagtatgt attttaaaga
     1621 ggtaaatttt gataacacac ccgagtttct agcttctcaa cactatatta acttttcaaa
     1681 aacagcatta gatacagatg tagtggctga tgaaaatgga aagaaatatg tgttagcagg
     1741 tagtttatta ggtgagagtg gcaaagtggt aaagataacc agaggaggtt cttcgggtag
     1801 ttatacatac acattatcag aagaccctgt aggaatagtt ttttcaactg tagatgttac
     1861 ttatggacca caaccagttg catcaatggt ggaaggctat gtaataatcg aaagattgca
     1921 aggtgagtat gtaaaagaag ctatagaaac tataaagaca aaattaccaa atattaaatt
     1981 tatgtaggag gatgaaatat ggcaagagta gaagaattat tgtcagttca agagctgata
     2041 aactatacaa agactagaaa attaaaagaa acaatgggag atttattatt tccaactcaa
     2101 aagatagaag gacttgaaat aaagatgata aaaggtgcat ctaatcttcc agtatcagca
     2161 agtgttcatg cttttgatac agaagcagag attgcatcaa gagaaggtgc taatttaagt
     2221 attgctgaac ttgcacttgt gaaaagaaaa attaaactag atgaaaaaga tataattgta
     2281 cttgaagagc caagaaactc acaagaagaa actcaaatga taaatcaaat atttaatgat
     2341 gttgataacc ttgtatcaag tgtaaatact agaatagaag caatgagaat ggaagtttta
     2401 tcaactggag aacttaatat aaatgaaaat ggagttaaag cttctttaaa atatggaact
     2461 ccaacaaatc ataaagaaac aaaaacttgg tctagtggaa caccagatat attaggagat
     2521 atttataata tgactgataa aatagttgtt gatactggat ttactccaac aaggtcatta
     2581 acttctaaaa ctattttaaa tataatctta agagatgaaa aactaagaaa agctatattt
     2641 ggtgtaaata gtgataaact acttacttta aatgaattaa atacattttt agtttctcaa
     2701 tctcttcctc ctatttttac ttatgatgaa agatacagag tacaaggcaa ggatggtaag
     2761 tacacaacaa agagattctt agatgaaaat aagtttattc ttatgcctga cggcaagatg
     2821 ggagatactt tctttgggct aacagcagag gaattagaac ttagaaaaaa cccagcaata
     2881 gatattagtt cagttggaaa tataattgta gaacaatact ctactgctga cccagttgct
     2941 aagtggataa aagcagttgc aacagcatta cctagttttc cttatgctga ccaagtgttt
     3001 atgggtacaa taaattagag gtgttgatat ggaagttgaa agactaaaaa aacttttagg
     3061 aatcagtaag gaagattatt caaaagagat gatattagag tttatattag aagatgtgga
     3121 ggaaatagtt aaaaattatt gtaatgtatc tgttattccc gaagggctaa atagtactgt
     3181 tttaaggatg gctatagata tgtataaaaa tgagaatcta ggaagtgaag atattgcact
     3241 aggttctatt tcttctatat cagaaggtga tacatcagtt tcatacagaa gttcagctag
     3301 tgaatttaag gaatcgttac tcaaagatta taagtcacaa ttaaacagat acagaaaaat
     3361 taggtggaaa taatgatgga taagactaga aaagcaatag aaatgttata cagagataaa
     3421 tgtactataa ttgagtatca gccagtcaaa gaccctgtga caaaacgaac taacaataaa
     3481 gaagtgattg tattagaaaa tcaaccttgt aagctttcat ataaaaacat taattctaca
     3541 gaagaaggaa aagtagctaa acttgcacaa accatcaaac tctttatatc tccaaacata
     3601 agcgttaaag caggttcaaa gcttattata actactcaaa ataacataac aagagagtat
     3661 gtaagaagtg gagaatcagc tatatatcca aatcatcaag aaataatact tgagttattt
     3721 aaggataaag cataatggct agatggggca gtgttgattt tagagagttt aaaagagttt
     3781 gtaaaaagat ggagaagctt acaaagattg atttagataa gttttgcaag gatgcagcaa
     3841 gagaattagc agcacgatta cttgggaaag taattagaag aacaccagtt gatacaggat
     3901 tcttacgaca aggatggaat ggagtggctt atgctaggtc gcttcctgtg tataaacaag
     3961 gaaataatta tattatagaa gttgttaatc cgactgaata tgcaagttat gtaaatttcg
     4021 ggcatagaac taaagatggt aagggatggg ttaaaggaca acatttctta acaatttcag
     4081 agatggaact acaaagtcaa gttgataaga ttatagagag aaagttatta atcttgttga
     4141 aaggagtatt tgatgcttaa taatataatt gatggaatat ctattaaatt agataaatca
     4201 tttggaaatg aatatacaat ttatagtgaa gatgtggagc aaggtataaa tgaaccttgt
     4261 ttttttattg ttcctttaaa tccaagcaaa gtatcatatc caagtggcag gacattaaaa
     4321 aagaactctt ttgatgtaca ttattttcct cgttcaaaag ataagagttt tgaaataaat
     4381 gaggtagctg agatgctact tgaggaatta gagtatatag aaattgatgg agatttagtt
     4441 agaggcacaa atatgaattt tgaaattata gataatgttc ttcatttctt cgttgattat
     4501 aactacttca ctataaaaag taatgataca gaaaaaatga atgatgttga attatttggt
     4561 ggtttgaaga gaggtgataa ttttgaataa aacattaagc aaagaagatg actacaagtt
     4621 tactaaggag caaatagtta attctaagaa gtatgtaaat agaaaagact tattaaatgc
     4681 aattttaaaa gaaaatgagt tatattcctt ctcagaagta gaggaaataa taaataattt
     4741 tatgaaagga gtgagttaga tggctttagg tggaggaaca tttgtaacac aaaataaggt
     4801 cctacctggt gcatatataa attttgtaag tgctacaagg gcaaccagtt cattatcgga
     4861 tagaggtatt gttgcaatgc ctttagagtt agattggggc atagatgaag aaattttcac
     4921 agtaaccagt gatgattttg agaagtattc aactaagtat tttggatatg attatactca
     4981 tgagaagctg aaaggtttga gagatttatt caaaaatata aggttgggat atttttataa
     5041 attgaataaa ggtgttaaag ctagttgcag tattgctaca gctaagtact caggtactag
     5101 aggtaatgat ttaaaagtta tagttacaac aaacattgat gataacacta agtttgatgt
     5161 tgtaacactt ttggataata agaaagtaga tacccaaata gcaaaagtta ttacagactt
     5221 acaggacaat gactatatta cttggaagaa ggatacaaca ctagaagcaa gtgcagggct
     5281 tgtatttact ggtggaacta atggcgaatc agtcacagga gcagagtatc aagctttctt
     5341 ggataaaata gaaagctact catttaatgc actaggcagt ttggctacaa cagcagaaat
     5401 taaaagttta tttgtagagt ttactaaaag aatgagagat aaagtaggag ctaagtttca
     5461 aactgtacta tataaaaaga atgatgcaga ttacgaaggt gtagtatctg tagaaaataa
     5521 agttaaagat actgggttat tagaatctag tttagtttat tggactactg gagctatagc
     5581 agtatgcgat ataaataaat ctaatactaa taaaaagtat gatggtgagt ttgatgttga
     5641 tgtaaattac acacaaatac aacttgaaga agcactaaag agtggtaaat ttatatttca
     5701 taaagttggt gatgaagttc atgtgttaga ggacataaat acttttgtat catttacaga
     5761 tgataaaaat gacgattttt caagtaacca aagtgttaga gtacttgacc aaattgctaa
     5821 tgatattgca actttattta atgaaaagta tttaggtaaa gttccgaatg ataaggcagg
     5881 aagaataagt ttctggaatg atgttgttaa acaccataaa gaattagaga atataagggc
     5941 aatagaagat tttaaaactg atgatgttag tgtggagctt ggaaatgaca agaaaacagt
     6001 tatagtgagt gatgcggtta aggttataaa tgctatgagt aagctttata tgactgtttc
     6061 agttagttaa ttaggaagga gaatattaga atggctaaaa atattactat gaagtcaaaa
     6121 gatgcaataa gtgcatcttt ggcagaatgc tatgttacca ttgaaggtaa aagatacctt
     6181 cttatgcagt gtatcaaggt agaggctaaa tttaagaaga ataaaagtaa gataccaatt
     6241 ttagggcaaa caggaaaagg aaacaaaact actggatggg aaggcacagg aagtgcaact
     6301 gttcattata atacttctat ctttagaaaa cttatgctta gatataagga aactggagaa
     6361 gatatatatt tcgatatgca aattactaat gaagacccaa caagttcagt aggtcgtcaa
     6421 actattatat taaaagattg caatatggat gaggttacat tagctaaatt tgatgcagat
     6481 agtgaatatt tagatgagga tatagatttt acttttgatg attgggatat gccagaagaa
     6541 tttaaaattt tattaggtat gatatagaat acacatttat aaattataga tgtgtgtttt
     6601 ttatgtaaaa attaaaataa aaggagaatt aagaaatgag taatttaagt gcttttttaa
     6661 gtcaaaatgc aataaaaaat gagaatgtaa tgtatgtggc aagcgataga tttttagacg
     6721 aaggaggaaa gccagttgaa tgggaattaa gagttttatc ttctgaggaa gatgaagtat
     6781 taagaagaaa ctgtaccaaa agagtgaaag tgattggcaa taatgggaag ccaacaggtc
     6841 aattcacaag tgaaattgac tacaatagtt atgtagcaga gttatgtgta gcatctacag
     6901 tatttccaga tttaaaggat gccgaactcc aaaatagtta tggagtgatg ggagaagctc
     6961 agttattaaa aacaatgctt acagcaggtg agtatgtaaa ttatacagta aaagttaacg
     7021 aagttaatgg atttgataca acatttgaag ataaagtaga agaagcaaaa aactaattag
     7081 gggcggtgat tttgatgcta gcatcactca ttattgtatc caaaaattaa agtggaaacc
     7141 tagtgaatat atgaatttag aagttaatga gagagcatta gcagccgcct caatacttgt
     7201 taagatagag gatgaagagg aagcaatgaa agaatctgaa agagagaaaa agagaggaag
     7261 gagaaggtag gcagggatta aatgaataaa gatatagaac ttacagaaaa agatttatat
     7321 tgtatcacta gacatattaa agtgtatatg ttcaaaaaaa tgatgaagtg ataaaaagag
     7381 aagataatcc atgttgtaga tatttatcac tgcaccacat ttaagaaatt atctaaaatt
     7441 acaggtctta aaatgggttt tggagtcaaa ctataatgta aaaattttta tggatatagt
     7501 ataatattta taaaaatatt gtacaggggg gaattattat gttttgctca aattgtggtg
     7561 cagaaatcac aggtataggc aagttttgtt cgaattgtgg ggctgctgta gaaaatgaga
     7621 ttattgaaga taataatatt aaatcaaatg atttaatcat tgatgctaat ggaatagaag
     7681 taaatatgac tgagatttat agaaaatata gaaaagaaaa agtaaatgca ataaaaaatg
     7741 taatggaaat aagcggtttg aatataaagg aagcaaaaaa aatagtggat tcttcctttg
     7801 aagagttaaa aaacaacttt attgatgata ctatgagtaa ttcagaaaag gaaaaaataa
     7861 caaacaatca aaatagaaaa aataatattg aaaaagctca acaagaatca gttgcttgtt
     7921 gtcctaagtg tggttcaaca tccttgacag ctcaaaaaaa gggttttggt ataggaaaag
     7981 cagtagtagg agcgagttta actggtggaa taggtttagt agctggaaat ttaggagcaa
     8041 agaaagttag agtaacatgt ttgaactgtg gcaagcaatt ttgggcaggc aaaaaataga
     8101 tgtattcata ataaaaacac ttactaaatt agtaagtgtt tttattatct aaattaacag
     8161 aaaggagagc agaaaaatgg caactataca aacatctatc cgaattttcg acggaatgac
     8221 acctgctttt agacacatga ctaatgctat gaatattgta ttaagttcat tcgagcaatt
     8281 acaaagaaca tctagcaatg ctatagatgc taatagtatt agaacagcta gagaagagtt
     8341 agcaagggca gaagctggat ttgatagatt agaacaacaa ataagagaag ctgatgaaca
     8401 acaaaaaaga tttaatgatg atgttagaaa aggagcaagt agtacagata agttagtaga
     8461 aaatgctaaa agattagtag caacttatat tggattgaga agtgttggtg gtttgattaa
     8521 tttaagtgac caaatgacga gtactaatgc gagactagct atgataaatg acgggcaaca
     8581 atctgatgga ggactcaaca aaatgatatt tcaatcagcg gaaagagcaa gagcatctta
     8641 tttagatact gcaaaaattg taagcagagt gggcatgaat gcaggcaaag catttagtag
     8701 tacaaaagaa atagtagcat tcgcggaaca acttaacaag aaatttgtaa ttgcaggagc
     8761 aacaacagaa gaaactaatt cagcactttt acaacttaca caaggtttag gaagtggtgt
     8821 gttaagaggc gaggaactaa atgctgtgtt tgagtcagca cctaatatta ttcagtctat
     8881 tgccgactat ctcgaggtcg atatagggaa gataagaggt atggcaagtg aaggaatgtt
     8941 aacagcagat attgtaaaaa actcattact tgcagcagca gagcagacca attcagagtt
     9001 tgaaaaaatg ccttacacat tttctcaaat ttggacttca attaaaaata atgcaatcat
     9061 gatatttggt gttatacaga aaaaaataga acaagctatg tctagtaggg gttttcgaac
     9121 ctttatagat aattttatag attcgttata tgttttagga gcagttgcat ttagtatttt
     9181 taatggaatt ataaatatat taggaagtcc attttttcaa gcatttgtaa atgctattat
     9241 tgtaggtgtt agtctgatag tgcaagtgct tggctgggta ataacacaag cattaaatat
     9301 tactaatgta tttgctcaga actggagtat tattgcacca atagtacttg gagttgcagc
     9361 tgctatgtta gtatataaca atgcactatt acttagtata gctaataaag taaaagatat
     9421 tgcgttatct gctaaaactt tagcgatgaa ttttgctcat ataatagcag agtcttatag
     9481 agcagcagca ttagtagcaa gcacaattgc acaggacgga ttgaacgcag caatggcagc
     9541 ttgccctatc acttggattt tatatggaat tatagccata gttgttgcct tttttgtagc
     9601 tatagctgta ataaataaat tcgcaggaac atcttattca gcaattggaa ttgttgcagg
     9661 agcattatcg ggtttaacag catttattat aaatagtgta tttttctgga ttaatgtttt
     9721 tatatcattt gctgagtttt ttacaaatgt attagaccat ccagtttatt cagtaaagaa
     9781 attatttttt aatttagcca cagcagtatt aaataatctt atatctatga caaaaggatg
     9841 tgatgaattt gctactaatt tagcaaactc tattatagat ggaattaatg gggcattaaa
     9901 agcttggaat aaatttgtag atgtcttaaa taaatttgga ggacttggag ataagttagg
     9961 acttggaaaa gctgatatgg ttggtcatac aaagtctatt acaagtacat tgcaaaaagc
    10021 taaaggagac ttaaataaat ggcttggagc agagccaaaa ggttataaat cctttaaaca
    10081 attagagtat aacaatgttg gagattgggc taaaaatgga tatgcctttg gtcaaaactt
    10141 agaaaataaa ataaaggata cttttgatat tagtaaaata gctgataagg caaaaaaaga
    10201 tttaggtctt gacgatttgt gggatgataa atatggatta ggtgatggaa taggctcagc
    10261 aggattaaac tcacctttgg gcgatgcagc aaaaggagca aaagataccg caggaaatac
    10321 agctaaaatg gctaaaacaa tggataaaag tcaagaagac ttaaaatatc ttagagatat
    10381 tgcagaacag gaaacaataa accgttttac aggagtcaac attaaaattg acatgaacaa
    10441 cacaaataac ataaataaag atgccgactt agatggaata gttaatgtac taacagaaaa
    10501 attaaatgac gctatggttg tatctgctga gggagtagtt tagaaaggag ggatataaat
    10561 ggcttatgac ttttatttag atggagtaca attaccaatc gcacctggca aattagaaat
    10621 aaaaataaca aataaaaata aaacagttga tttgataaat actggagaag taaacatact
    10681 aaaaaaagaa gggttatccg agataagttt tgaagcagaa tttacacata ataaattacc
    10741 tttttatcgt ggagctttta aagatgttca attcttttta agtaaattag aactattaaa
    10801 aacagattgt aagccattcc aatttattgt atcgagggaa ttaggtaata aagtactatt
    10861 taacactaat ataaaagtat ctcttgaaga gtatgctatt tcagaagatg cagaaaatgg
    10921 ttcagataca aaagttgcaa taaagttaaa gcaatataga gattactcaa ctaaaaagtt
    10981 agttcttgcc cctcctaaaa atgagactgg tagaccaagc gtaaagatag agccaaaacg
    11041 agttgattca gtcaatgcca caaacactaa aactaaaaca tatacagtaa aagcagggga
    11101 tagcctttgg tcaatttgtc agaaacaact tggtaatggt tcattatata agaaagtata
    11161 cgaactaaat aaatctatga tggataaggc aaataagggc aaaaacttaa gtaaatacac
    11221 tatttacaaa gggcaggtgt taaaacttgg ttgatgaatt agtgttagca aatgatagag
    11281 atgtaaggtt agttattgca cattgggaag atttctacga acctgctgtc attgatggta
    11341 tcacatggga aatagaaaga cgaggaacac cttctaagtt agagtttact atagtcatgg
    11401 atgatatact acagttttgt gaaggtaatt cagtgagatt atattacaag ggtgtaggca
    11461 tattctatgg atatatattt cagaagaaaa gagataaaga aaatcacatc aaaattattg
    11521 catatgacca gttaagatat tttaaaaata aagatactta tgtatatagt aataaaacag
    11581 caagcgaact tataaaaatg ctcgctaaag attttaattt aaaatacaat gtcatagaag
    11641 atacaaaata taaaatatct agaatagaag aaaacaaaac tttatttgat atgattttaa
    11701 ctgcactaga tgatacttta agagaaaaaa aagaaatgtt taccttgtat gatgactttg
    11761 gaagaataac actaaaaaat gttgcatcaa tgaaacttga tactgttatg aataatgatg
    11821 taatcgagga ctttgactat aattcttcta ttgatagtga tacttataca aaaatcaaac
    11881 ttgtaagaga taatgaagaa acaggaaaaa gagatgtgta tattgctcaa gactcaacgc
    11941 atatgaggag ttggggaata ctacaactat ttgatacagt tgataaaaat atgagtgaag
    12001 cagagataaa acaaaaatgt gatatactcc taaaactata taataagaaa actaagtcat
    12061 taagtttaaa aaatgtgtta ggagatatta gagtaagagc aggttgttta gtacctgttt
    12121 ttttgtcgtt aggagatatt gatttacaaa attatatgtt agttgagaaa gtaaaacata
    12181 catttgaaaa taacagtcat tttatggacc taactttggt tgatggagac gaatttgctt
    12241 cttattcttc atcaagttat agtagtggaa atactaataa taaaaatgag aaacaaaatg
    12301 gtcctgcaca aagtactaca agtaaagaag atactgatat ggctaataag attaataaac
    12361 tacttaaagg taaattatca aatacaggaa atatatttgt taaatattca aatgcttata
    12421 aagttaatcc agcactcatg gctgctatat ctatgcacga atcagctaga gggacttcaa
    12481 atattgcaaa tactaaaaat aatttctttg gaatgaaaaa aaatggagat tacatgagtt
    12541 tttctagtgt agacgaagga ataaaaagag gtataagtaa tttatcaaga aactatatcc
    12601 atataggacg aaaaacttta gaaagcatca gaaataaata ttcttctagt tcagacaaag
    12661 aatgggtaaa atgtgtaggt gcattttata agcaaataac aggaagtact tataattcta
    12721 atagtgcagg cacaggagtt ggaagtaatg aagaagcaga aaagaattta aaagatttaa
    12781 cttatcaagt tcaaaacaat aattctaata catcaacaaa caataataat aaagtaagta
    12841 aagttattca agaagcaaaa aatcaacttg gcaagcctta caaatggggt ggtaatggtc
    12901 caaagagttt tgactgtagt ggtcttatgg tgtgggcatt taaaagaggt gcaggaataa
    12961 atctcaaaag agtttcagca gaccaatcaa aagatagtag aggaaaacta ttatgtaaca
    13021 taaatgatgt aaaagctggt gatttagtat tctttgcata caacaaagga aaaggaaatg
    13081 tacatcatgt tggactatat ataggaaatg accaatatat tcatgctcca caaactggtg
    13141 acgtagtaaa aataagtagt ttaagtggta gacaaaagaa aaagcatgat tttgcaagag
    13201 ctagaagatt cttttaagtg aggtgataaa gtgtcacaag aattattgca aataattaag
    13261 aaggctgcaa tggatgcagt agaaacaagc aatccaatgc aaattgcatt tggaactata
    13321 gaaagtgtta atcctttgat agttaagata gaacaaaaag catcttttga agaatttttt
    13381 ctaatacaaa cagagacttt taaaagatat acagataaaa aaatagggga taaattagtc
    13441 ttaattagga tgcaaggagg acagcaatat ttgattttag ataggatgtg atgaggtgtt
    13501 accaagcgat aatttggatt atgacattga agatgtatca ataattaatt ttgatgtaag
    13561 gcaagaacca agtaagacct ttaaattaaa tatagaaaaa tctaagatag atggtatttg
    13621 tgatgatgtt gaagcattaa aacagaccat ctttttaatt ttaaatactg aaaggtatga
    13681 gcacctcatt tattcttgga attatggagt cgagttgaac gaccttattg gagaacctat
    13741 atcctttgta atccccgaac ttgaaagacg aatcaaagaa gcactaattc aagatgatag
    13801 ggttgaaaat gtagataatt ttgaatttca aaatgtaaaa ggaaaagtac attgtaagtt
    13861 cacagttcac agtaaatatg gaaatataaa agcagagaag gtggtgagtg tataattgtt
    13921 tgagttaatg acatttgaaa atataattaa aagaatgtta gatagtgtac cagatacttt
    13981 tgataaaagg gaaggttcta taatatataa tgctcttgct cctgttgcta tagaacttac
    14041 agaaacatac attgctatgg atgaattact agaccaaaca ttcgtagata ctgctagtta
    14101 ttattacttg gagaaaagat gtaaagaacg aggaattaca ccactatctg caactaatac
    14161 cattgcaaaa ggagtgttta atatagatat tccacttgat tctaggttta atctaggaga
    14221 atacaattat attgcaattg agagaatatc tgaaaaaaca tataaaatga aatgtgaaac
    14281 tgctggacct atatttgagt taggaaaact aatacctatt gaatatatag atggtcttga
    14341 aactgctgaa ctaactgaaa tcttgataaa tggagaggat gaagagagtg aggatagttt
    14401 aagacaaaga tattatgata gcctaaattc acagagcttt ggtggaaata tgcaaaatta
    14461 taaagatgaa gttaacaaaa tacaagatgt tggaggagtt aaggtttatc ctgtgtggga
    14521 cggtggagga actgttaagt tagtaataat taactctaat ttcaaagtac catcagagga
    14581 tttagttaat ttagtgcaag aagaaattga cccaattgga catcaaggac aaggcttagg
    14641 attagcacca atagggcata aagttactgt tacaggtgtt gtaagtacaa ctataaatat
    14701 atcagcagag ataacataca aaaatggcta cacttgggag aatataaaat caattgcaga
    14761 agaagcaata gacgactatt taaatgaact taacatgagt tgggaagatg aagaaaactt
    14821 aatagtccgt atatctcaaa ttgaaactag attacttagt attgatggag tattagacat
    14881 tgcaaataca atgataaatg aggttaaatc taatctaaca ataaatagta acagtatagt
    14941 agtgagaggt gaggtagttg gataaagaga ttaatctaat aaattactta ccacaaattc
    15001 tacaagataa agaagaatat ataaaagtat ttaatgtagg aaataaagaa ataaaaatat
    15061 tacatgataa attaaaggac ctatcaaatg accagttttt agaggaccta actataagtg
    15121 gtataaaaag atgggaaaag ataatgtcta taactcctaa aagtaatgag agtttagaag
    15181 atagaaggtt taggattttt agtaaatata taagtaaatt accttactca gagagatttt
    15241 taaggaattg gctagatagt atagttggag aaggtaatta tgagttaact at
//
LOCUS       DAYAOY010000100        15200 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYAOY010000100 DAYAOY010000000
VERSION     DAYAOY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.100"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            2..571
                     /locus_tag="ACGI6B_003168"
     CDS             2..571
                     /locus_tag="ACGI6B_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752584.1"
                     /translation="MKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNEVNSQDTTTID
                     VVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQESNQNNIMNVK
                     NTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVLFVVRNFTTTT
                     SASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(757..1629)
                     /locus_tag="ACGI6B_003169"
     CDS             complement(757..1629)
                     /locus_tag="ACGI6B_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9752585.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            2046..3449
                     /locus_tag="ACGI6B_003170"
     CDS             2046..3449
                     /locus_tag="ACGI6B_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752586.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            3476..5020
                     /locus_tag="ACGI6B_003171"
     CDS             3476..5020
                     /locus_tag="ACGI6B_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HFG9752587.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            5051..6442
                     /locus_tag="ACGI6B_003172"
     CDS             5051..6442
                     /locus_tag="ACGI6B_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFG9752588.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            6635..8131
                     /gene="abfD"
                     /locus_tag="ACGI6B_003173"
     CDS             6635..8131
                     /gene="abfD"
                     /locus_tag="ACGI6B_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HFG9752589.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            8266..8541
                     /locus_tag="ACGI6B_003174"
     CDS             8266..8541
                     /locus_tag="ACGI6B_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752590.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            8565..9872
                     /locus_tag="ACGI6B_003175"
     CDS             8565..9872
                     /locus_tag="ACGI6B_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HFG9752591.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            9892..11010
                     /locus_tag="ACGI6B_003176"
     CDS             9892..11010
                     /locus_tag="ACGI6B_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HFG9752592.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            11297..12217
                     /locus_tag="ACGI6B_003177"
     CDS             11297..12217
                     /locus_tag="ACGI6B_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFG9752593.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            12332..13489
                     /locus_tag="ACGI6B_003178"
     CDS             12332..13489
                     /locus_tag="ACGI6B_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFG9752594.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(13574..15073)
                     /locus_tag="ACGI6B_003179"
     CDS             complement(13574..15073)
                     /locus_tag="ACGI6B_003179"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFG9752595.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5842 a         1826 c         2752 g         4780 t
ORIGIN      
        1 tataaaaaaa gcctggtata aaatcaaatt aattgataga tgccttataa ttgtaatggc
       61 aatattaatg tttcaatcta tatataattt atttgttaat gaagtaaatt cacaagatac
      121 tacaactatt gatgtagtag taagaaccac ttcagcagct atatttgggt attttttaag
      181 tgctaatttt ataaagagac ctttaaggaa gtatacaaat actgatgtat cttcaaatct
      241 ttttatagat aataatttat ccccaaaagg tcaagagtct aatcaaaata atataatgaa
      301 tgtcaaaaat accattggat ttacttcaga agctgataat tatgaaaaga aaatacctat
      361 taataataat gaaggttttg aagaaggaga aactagtgaa ttgcaaatta ttatagctac
      421 tgtaatttgt atagtagcat taacagtact ttttgttgta agaaatttta caactacaac
      481 atcagcttct ttaggtacta tttcacagat gagagatttt gtctcaggat gcgtgggttt
      541 tttgctaggt tgtccaagta aacctgatta aaaaagtatt atcaataaaa ggtattttaa
      601 aaagtatcag tataataata tttagattta aatgtataca attataattc taaacattct
      661 atattgtatt tttatactga tactctaaac tatactattt attaaaatat tttaacattg
      721 tatttaaaca aaatattata tctgtataat ataaatttag tttatatgaa tagatgccat
      781 atactcaata aaaagtttca tactatgaga tatgtattga tttcttctcc aaagaacccc
      841 aatattcgca gtaattgcac gttctaatgg tattgaacag agattttctg tattttgaaa
      901 tatttcttta tacaatattg ttgaagccaa gtcattttct agtaaataac gtatggtaga
      961 aagttgattt gagtgtaaaa ttatatttgg cttagctttt tcaagtcttt ttgtaataat
     1021 cttatagtga taagaacctt cagataagat tacaaaaggt acatttttta acatataatt
     1081 cgatataatg ggatgttttg ccaattcatt tttgggattt gtaactaagt ataattctgt
     1141 tttatagatt gtctttgaat ctatatcatc ataaatatca ctgtcaatta taccaataca
     1201 aaaatcaagt gtagattctt caatcatttt agctccaaca aatgttggaa cttcaaaaat
     1261 tttaagctct atatctggat aaattacatt aaatctagga agtatttttg aaagcaaaaa
     1321 cgtacctaaa actgtaggaa tacctatttt gagagaagct tttctctttt ttcctaaatc
     1381 aagtgcgtca cattgaaaat cctcaaattg ttttataaaa tgttttgact tgtctaaaaa
     1441 aatttttcct tcaggcgtta actctatttt attacctatt ctgtaaaaaa gttcaaagcc
     1501 aagttctttt tctaattctt taattgctaa tgttatggat ggttgagaaa tatgaatact
     1561 ttccgcagcc ttagtcatgt ttttatattt acagacagtt tgaaaatagt ataaatagtt
     1621 tatattcatt tggtattagt tcctttcatt tacatattag tgacaagtat ataattaaca
     1681 atagcataag ttttgtatat tgttgtaaaa aaatagaata gttttaaact atcatagcat
     1741 aagtttttga tattttcaat ctattaagaa tagtgctatt attaaattaa caaaagagaa
     1801 tagctggtat ggtttagagt aaatcaatct atattacagt tatatcaacg aatatcaaaa
     1861 aattatctat tagaaaataa aaattatttt tacaattaaa ttgttaatta aatcataaag
     1921 aaaaaagtga tattaacaag ataaaatttt tttaatcaga aatgttaatt taataacaaa
     1981 atagagtatt ggggagaaag gagaaatata ttttttaata atatttgtta atttaataac
     2041 gaaaaatgaa agggagtgat ttagtgaagt ttgaattgat ggatttttta atgaatcctt
     2101 ttgtccttat gtttgctgca gtaattacag gaattttatt tggaaaaatt aaatttggga
     2161 agtttaattt tggggtatca ggagcattat ttacaggatt atttattgga tggcttgcat
     2221 atagcttggg gaatctaata atagcaaaag gggaaacagc agcaggatat aaggcagcaa
     2281 ctgtaatgat gggtaatgga attatttctt ctgacttctt tgatttcttt ttaataatat
     2341 ttgttgcagc agttggtcta ttggcagcaa aagatatgaa agctgtatta aagaagtatg
     2401 gagcaagatt tgtaatttta ggagttctta taacttttat aggtggctct atgacttatg
     2461 caatgacttt actatcaagt gataaaggaa gtagtgctta tgaagtttct ggcgtttaca
     2521 caggagcatt aacaagttcg cctggattag ctgctgcttt agaaacagct ggtaaacatg
     2581 cagaagatgt atcaaaagaa tttgaaaaag cttcaataaa agataaaaaa gaaattttaa
     2641 aagtagtcga cccagaaggc aaattggatg tgaatacaac tacatcactg actcaagagc
     2701 aaatagataa gtatattgca tatgctgaag ctggtgttgg tataggtcat gcagtcgcat
     2761 atccttttgg agttttaata gtaatcttag gtgtaaattt ccttccaaaa ttatttagga
     2821 tggacctaaa agaagaaaga agaaaatatg agaaggaaat gaaggaagca agagatagtg
     2881 tttctggtaa aacgatacca gaagttccat ttaatataat aacatttttc ttgacatgtt
     2941 tagctggata cttagttgga ggaatacatg tatttatggg accactaggg tacttcacac
     3001 taggcgcaac aggaggttca ctaattgtat ctctagttct tggatatata ggtaagatag
     3061 gggtagtaaa tttcagaatg gaagaaaagg ttttaaatat tttaaaacaa atagggctag
     3121 tatttttctt agcaatagtt ggattaagat atggtggaaa agtagttgat tcaattatga
     3181 catctggtat gcacctagcc ttagtagcaa tagcagtagg tgttacagct atgatgatag
     3241 gattcttagt tggcaaatac gtatttaaat taaattggat tttattatct ggagctgtct
     3301 gtggtggtat gacatcaaca ccaggtcttg gagctgctgt tgatgctctt gatagtgatg
     3361 acccagcagc aggttatggt gctacgtatc catttgcatt attaactaaa gttatattgg
     3421 ttatagtttt acacaaactt ccaatgtaaa taagatttta tatgggggga tatttatgaa
     3481 taatgaagga gcatttgaag aattaagtgg aagattaaga aataaagaat tgtgtgattt
     3541 aataatgaat gcagatgaag ctgctaaaat tatagaggat ggcatggtta ttggagttag
     3601 tggatttaca ccttcaggct atccaaaagc agtaccatta gcagtttcag aaagagctaa
     3661 aagtggagaa aagataaaat taaccgtata ttcaggagca tctttaggtc cagaagttga
     3721 tggagcatgg tcagaagcag gtataattga aagaagactt ccttatcaaa ctaattcaat
     3781 acttagaaat aatattaata aaggtgttgt tgattatata gatatgcacc taagtcattc
     3841 tactcaattt ttaaattatg gaactatacc taaagtagat gtagctatag tagaagcact
     3901 tgcaataact gaagaaggaa atattatacc aactagtggg ataggtaatt ctccatcttt
     3961 tataaaaagt gcagataaag taatagtaga gataaatctt gcaaaaccaa tggaaatgga
     4021 aggtatggca gatatatata ttacggaaaa tccaccaaat agaaaaccaa tagaaataaa
     4081 tcatcctaag gatagaattg gaacaacata tataccttgt ggtttggata aaattgcagg
     4141 agtagtaatt accaatatgc aagataagac ccgtccattg ggagttgtag atgaagcttc
     4201 gaaaaaaata tcaaataata ttattgcttt tttaagagaa gaagtaaagg caggcagatt
     4261 atcaaagaat ttacttcctc ttcaatctgg tgtaggaagt gtagcaaatg cagtattata
     4321 tggattatgt gaatctgaat ttgaaaattt aacttgttat acagaggttg tacaggactc
     4381 tatgttggat ttaataagaa tgggtaaagt tacaatggca tctactacat ctgtaagtcc
     4441 atctccagaa ggattaataa aatttgaaaa agatattgat ttttttaagg ataagattat
     4501 attaagacct caagaaatta gtaacaatcc agaaatagcc agaagaattg gtgtaattgc
     4561 aatgaacact gcaattgaag ttgatattta tggaaatgta aattctactc acataatggg
     4621 gtcaaagatg atgaatggaa ttggtggttc tggtgatttt gctagaaatg gagcaataac
     4681 tatatttagt actgagtcaa ttgcaaaaaa tggtgatata tcatcaatag tacctatggt
     4741 atctcacgta gaccacactg aacatgatgt aatggtaatt gtaacagaac aaggatatgc
     4801 tgatttaaga ggtcttgcac ctagagaaag agctataaaa ataatagaaa attgtgccca
     4861 tccagattat aaagaacaac ttagggatta tttaaataga gcttgtaaaa gtggagcaaa
     4921 acaaacacct cacatattgg acgaagcttt atcatggcat agtaaattta tgagtacagg
     4981 tacaatgaaa aaggctgaaa cttttaaaag tgcattataa aatatttaat aaattggagg
     5041 tagattttaa atggaaaaag cagtggaaaa ctttgaagat ttaagtaagg aatacattaa
     5101 tggttatatt gagagagcaa gaaaagctca aagagagttt gaatgttata ctcaagaaca
     5161 agtagataaa atagtaaaaa tagttggaaa agtagtgtat tataatgctg aatatttagc
     5221 taaacttgct gtagaagaga ctggaatggg agtatatgaa gataaggtag ctaaaaataa
     5281 aagcaaagca aaagttatat ataacaattt aaaagataaa aaatcagttg gtataattga
     5341 tatagataga gaaactggaa taacaaaggt agctaaacca gttggagtag tagcggcaat
     5401 aactccttgt acaaatccaa tcgttacacc tatgagcaat gcaatgtttg cacttaaggg
     5461 aagaaatgca attattatca cacctcatca taaagcaatt ggatgtagta caaaaactgt
     5521 agagatgatt aatgaagaat tagaaaaaat aggagctcca gaaaatttaa tacaaatact
     5581 tgaccaacaa tcaagagaaa atacaagaaa tttaatttca tcagcagatg tagtaattgc
     5641 cactggagga atgggaatgg taaaagctgc gtactcaagt ggaaaaccag cacttggtgt
     5701 aggggcagga aatgttcaat gtatcataga tagagatgta gacattaaag aagcagtacc
     5761 taaaatcata gcaggaagaa tttttgataa tggtatcatt tgttcaggtg aacagtcagt
     5821 aatagtagca gaagaaatgt ttgataaaat aatggatgaa tttaaaaata ataaaggttt
     5881 tatagttaga gataaagtac aaaaagaagc tttcagaaat gctatgtttg taaataaatc
     5941 tatgaataaa gatgcagtag gtcaatcagt tcatacaatt gcaaaaattg caggtgttga
     6001 gataccagaa gatacaaaaa taatagtaat agaagctgat ggaccaggag aagaggatat
     6061 tattgcaaaa gaaaaaatgt gtccagttat atctgcatac aagtataaaa gttttgaaga
     6121 aggtgtagct attgcaaaag caaatttaaa tgtagaagga aaaggacata gtgtatctat
     6181 acattcaaat acagttaaaa atatagaata tgcaggagaa aatatagaag tatcaagatt
     6241 tgttattaat caatgttgtg ctactagtgc tggaggaagt ttctttaatg gtcttgcacc
     6301 tactaatact ctaggttgtg gttcttgggg aaataacagc atctctgaaa atcttgatta
     6361 taagcatcta attaatattt ctagaattgc ttattatatg ccagaaaatg aagtccctac
     6421 agatgaagaa ctttggggat aaaatttaga caaaaaatat tcattctaaa ttaaagtagg
     6481 aatatatttt aatttagaat gaatttaaga cataaattaa atttaaatta gtttattgga
     6541 ctaaatttaa aatattaaga gacatataga agaaggaagt attataattt aaaataaata
     6601 aaaaaatcta taaaagagaa ggagtggttt tattatggca ttaatgacag gagctcaata
     6661 tattgaaagt ttgagaaaat taaatactaa ggtttatatg tttggtgaag aggttaagaa
     6721 ttgggttgac catcctatga ttagaccatc tataaattgt gtagcagcaa cttatgactt
     6781 agctcatgac ccagaatatg cagatttaat gacagttact tcaaatatca caggtgaaaa
     6841 aataaataga tttggtcatc ttcatcaaag tgttgatgat ttgataaaaa aagtaaaaat
     6901 gcaaagacta tgtggtcaaa aaacagcttc ttgtttccaa agatgtgttg gtatggatgc
     6961 gtttaatgca gtgtattcaa caacatttga gtgtgataaa gcccatggaa ctaattatca
     7021 tgataatttt gtaaaatact taacttatat acaagaaaac gatttagtag ttgatggagc
     7081 tatgactgac cctaagggag atagaagtct atcacctagt gctcaacctg acccagatat
     7141 gttccttcat atagtagaaa gaagagaaga tgggataatt gtaagaggag caaaggcaca
     7201 tcaaactggt tcaataaatt cacatgagca ccttataatg cctactataa gtatgactga
     7261 agcagataaa gactacgctg tatcatttgc agttccttca gatgctgaag gagtgtttat
     7321 gatttatgga agacaatctt gtgacactag aaaattagaa gaaggtgcag atgttgattt
     7381 aggaaataag gagtttggag gtcaagaagc tttagtagta tttgataatg tatttgttcc
     7441 aaatgataga attttcttat gtggagaatg ggatttctct ggtatgttag tagagagatt
     7501 tgcaggatac catagacaaa gttatggtgg atgtaaagta ggagtaggtg atgtaataat
     7561 aggagcagca gctttagcag ctgattataa tggagcaaac aaagcttcac atataaaaga
     7621 taaactaata gaaatgactc accttaatga aagcttatat tgctgtggta tagcatgttc
     7681 atcagaaggt cacaaaacag aagctggaaa ctatcaaata gatttacttc ttgcaaatgt
     7741 gtgtaaacaa aatgtaacta gatttcctta tgaaatagta agacttgcag aagatatagc
     7801 tggagggctt atggttacta tgccttcaga aaaagacttt aaatcagacc ttaaagttgg
     7861 aacaagtgga atgactattg gcgaagtatg taacaagtat tttaaagctt cttcagtggc
     7921 ttcaacagaa gaaagaatga gaatattaag attcttagaa aatatatgcc ttggttcatc
     7981 tgctgttggt tatagaactg agtcaatgca tggggcaggt tcaccacaag ctcaaagaat
     8041 aatgatttca agacaaggaa acataaatca aaagaaagaa ttagctaaga aaattgcggg
     8101 aataaaaaaa gaggaagctt taaatttata aaaagttgat atataaaggg aaattatata
     8161 ttaatgtgtg ttaattaatg aaaaaatatg tagtataaga gatagttata aaattatggt
     8221 ggtttttagc taagtaaaga aatttttaat aaggagaatt tacacatgac ctgtggagtt
     8281 agattttgtg ggggctgtaa ccctagatac gaaagaggga aggctcttac aataatcaaa
     8341 gagcacttca atggtagggt agattttagt atagcacaag agaacgtcga gtatgatttg
     8401 ttacttataa ttggaggatg cgcaactcta tgcgcatcct atcaacaata taaagtgaaa
     8461 aatgaatgtg ttaagatgtg ggattttagc catatagaaa gagttataaa tgtgattgaa
     8521 tcagcaattt gtgaaactta aaaaagctaa atggggggat ttcattgagt tggcaagaat
     8581 tgtatcaaag taaattatgt agtgctacag aagctgtaaa acagattaaa aacggtgata
     8641 ctgtagtatt tgcacattgt gtaggtgaac ctcctgcact agtagaagct atgattgaaa
     8701 atgctgaaca atataaagat gttgagatta aacatatggt aagcttgggt agtggtggat
     8761 atactgctaa gggaatggaa gctcatttta gagtaaatcc aatgtttgta agtggaaatg
     8821 taagaaaggc aattgaaaat ggagatggtg attttacacc tgcattcttc catgaagtac
     8881 caaagttgct tagagaaaaa agattaaaat gtgatgttgt tttagcacag gtaactccac
     8941 cagatgaaca tgggtattgt agcttaggaa catcagttga ttatacttat gaagccatta
     9001 aaactgctag aacagtaatt gttcaagtga atgaccagtt tcctagaacc tatggtgagg
     9061 tagtccatgt atcagagttt gactatatag tggaaaaatc acaaccttta tttgaactac
     9121 aacctgcaaa gattggagaa gtagaagaag caattggtaa aaattgtgct tcattaattg
     9181 aagatggctc tactttacag ctagggattg gtgggatacc agatgcagtt atgttatttt
     9241 taactgataa aaaggattta gggattcatt cagaaatgat ttcagatggg actttggccc
     9301 tttatgaaaa aggtgttata aatggtaaat ataaaaattt tgataaagaa aaaatgacag
     9361 ttaccttctt aatgggtact aaaaaattat atgactttgc aaataataac cctgcagtag
     9421 aggtaaaacc agtagactat gtgaatcatc cagcaattat aatgaaacaa cataagatgg
     9481 tttctataaa ttctgctata caagttgatt taatgggaca ggtagttgca gaggctatgg
     9541 gacttagaca atttagtggt gttggaggtc aagttgactt tatacgtgga gtgtctatgg
     9601 gtgaagatgg aaaggcaatt atagctatgc cttcaataac tacaaaaaaa gatggtacag
     9661 taatttcaaa aatcgtatct attgtggatg aaggtgcacc tataacgaca tcaagaaatg
     9721 atgttgacta tattgtaaca gaatacggta tagcagaatt aaaaggaaaa tctcttagag
     9781 aaagagcaag aaatttaata aatatagctc atccaagtgt aagagaacat ctagcagtag
     9841 aatttgaaaa gagatttaag gagaaatatt aataatagag gggtgttaat aatggaagca
     9901 cttagagtag ttccaaagat attttacttt gatacattta aagaatttaa tgaagaattt
     9961 aaaataggaa aaaatgattt agtaattact aatgagttta tatatgagcc atatatgaaa
    10021 cctcttggaa tagatacaaa tttaattttt caagaaaagt ttggaacagg tgaaccatct
    10081 gatgaaatga tagactccat gacaaaggaa atgaagaaat acaattttga tagaattatt
    10141 gcatttggag gaggaacaat agttgatata tgtaagatat tagcacttga tgttccagaa
    10201 aagtctattg atttatttga aggagaagta tcacctaaaa aagtaaaaga gttagtagta
    10261 gttcctacaa catgtggtac aggtagtgaa gtaacaaatg tagctattgc agagttaaaa
    10321 tctaaacata ctaaaaaggg gcttgcagta gaagaaacat atgcagacta tgcagtgtta
    10381 attccagaga cagtaaaagg gttaccttac aaattctttg ttacaagttc ggtagatgct
    10441 cttatacatg ctattgagtc ttatctttct ccaaaagcaa gtccatttac agagatgtat
    10501 tctctacaag caataaaaat gattatggat gggtataaga agatagttga taaaggagaa
    10561 gaagagagat ttaatcatct tagagacttt gtacttgcat caaattatgc aggaattgct
    10621 tttggaaatg ctggttgtgc agcagttcat gcactatctt attcaatagg tggagctttc
    10681 catgttgcac atggtgaagc aaattatcag ttctttactg aagtatttaa gatgtattct
    10741 agaaaaaaac ctaatggaaa aattataaaa tgtacaaaaa ttttagcaga tgcccttgaa
    10801 tgtgacccta actgtgatgt ttatggagaa ttagaaaagt ttttaaataa attaatagct
    10861 aaaaaagctt tgagagaata tggaatggtg gaaagtcaga tagatgaatt tacagatagt
    10921 acaatagcaa atcaacaacg actacttgct aataattatg tagagcttag tagagaggaa
    10981 ataagagaga tatttgcaaa tctttattaa tacaattttt tatttatatg tatagtcaac
    11041 ataaacaatg ctaattatat ttatgtacaa ataaaaataa gggaacttaa attttaatta
    11101 aagttaagtt ctcttatttt tatatggagt taaatataag tatattttta atttaaaata
    11161 tgcttatatt taagtctatt actttatttg aaacaatttg tagaatttca aattccttga
    11221 cagtaataag taagtaattg tataatcaaa ctgcaatata ttaataatat tagtgaaaat
    11281 gaaaggatta taaaatatgt ataaatcaga aaatttttca aaaaaatctg tattaataaa
    11341 ttcattttta agtaaatttt taaaaataat tggatggggt ttggtaagct tatttacatt
    11401 atcagcctta gtactttcaa ctatttccat tataattgga tgggctgtaa tacttcctat
    11461 tcaagtatgt gcagtatttt tacttatagg aggagtttgt atttttaaag gtaagagatt
    11521 aggagaccaa atttctcgtt ataaaaaata ttgtacaatt ataaacaata gaaatattat
    11581 tccagtagaa cttattgcag aaaacacatc taaatcatta aattttataa taaaagatgt
    11641 acaaaagatg atagacaaaa actactttat aaatacttat attgataaaa gaaataatca
    11701 gattgtgtta acaaatgaag attttgttcc accaagatat gaagatgtag catatgaagt
    11761 caaagaagaa gaaaagactg atgaatccaa tgaagttgat acaattataa ataaaggaat
    11821 gaattatcta acacaaataa aagaagctaa taaaaatatt aaatcagaat caatgggcga
    11881 taaaattgta caagtagaag atgttacaag taaaatattt gatgttgtaa aacatgaccc
    11941 atcaaaactt actcagatac aaaaatttat ggattattat cttccaacca ctttaaaact
    12001 attaaattca tatcatacat tggaagaaca aggaattgat agagaaaata taacaacaac
    12061 tatggaaagt attgaaaata caatgaatac gattgtagta gcttttgaaa atcaattgga
    12121 ctatctgttt gaagatgaag ccattgatat ttctacagat attactgttt tagagaatat
    12181 gttagtccaa gaaggattaa cgagtggtaa attttaactt caatagaaaa aatattctgt
    12241 tatttttaaa caattaatta gtcataaagt ataaaatgtg tattatataa gttaatcagt
    12301 actttatgaa ggaagaggag agaaagatac aatgaataat ttagatgata taccagttat
    12361 gccaactttg acacttgacc catttggaga agaatcaaac actaatgata tagataattc
    12421 tgacttatta atgaaaaagg atgaaaaaga cccagaagaa gaaaaactgt ctgaatctga
    12481 acgcaagatg gtaaaggaat ttgcggaaaa aatagatatt acaaatacaa atatgatttt
    12541 gcaatatgga gctggagctc aaaaaaaggt agcaagtttt tctgaaacag ctttaaagag
    12601 tgtaaaaaca agagatttag gagaagttgg agatatgctt acaaatctag ttactgattt
    12661 aaaagcattt tcagcagatg aaaaagaaca aagtggtttt ttaggaattt ttaaaaaggc
    12721 aaataataaa atttcaaatt taaaaactaa gtatgatagt gcagaggtaa atgtggataa
    12781 agtaagcaaa gagcttcaaa agcatcaggt taaaatattg aaagatatag caatgcttga
    12841 taagatgtat gaattgaact tagcttactc aaaagagctt actatgtata ttatagcagg
    12901 aaaacaaaaa ttaaaggata tgaaagaata tgaaatgcca aagttgagag aaaaagctcg
    12961 tttatctggt tcaacagaag atgctcaatc tgtaaatgat atggtttcac tttgtgacag
    13021 atttgaaaag aagattcatg acttagaatt aactagaatg gtatcgcttc aaatggctcc
    13081 tcagataaga ttggttcaaa atagtaataa tctgatggca gaaaagattc aatcaactat
    13141 agtaaatacc attccattat ggaaaaatca aatagtgctt gctcttggaa tttctcactt
    13201 aaatcaagct atgaaagcac agcgtgaagt tagtaatatg acaaatgagc ttttattaaa
    13261 gaatgcagaa actttgaaga tgggaacaat agaaacagca aaagaatcag aaagaggaat
    13321 tgtagatata gacacaatta agaaaactaa tcaatctcta atatctacta ttgatgaggt
    13381 agttaaaata caacatgatg gtagaataaa gagacaagaa gcagaagttg aattatcaaa
    13441 aatagaaaat gaacttaaaa gtaagcttct tgaatttaca gttaaataaa aattatacta
    13501 atataaaaag agactgtctc aaaatagaga taagtctctt tttcattgtt taaatgatta
    13561 aagtatattt atattataaa tttctattat cttttaacat aacatcatga gctccacctt
    13621 caactatact agtagaagat acaagtgtaa gtttagcatt tttttgaagt tcaggaatgc
    13681 taagtgctcc acagttacac atagtagaac gaactttact taaagaaaga gttacattgt
    13741 ctttcaaaga accagcataa ggcacataag agtcaacacc ttcttcaaaa gaaagtttct
    13801 tatctccacc aaggtcatat ctttgccagt ttctagcacg agcagaacct tctccccaat
    13861 actctttcat ataactacca tttatattaa ctttgttagt tggactctca tcaaaacgag
    13921 agaagtatct accaagcatt ataaaatctg cacccatagc taaagctaaa gttatatgat
    13981 ggtcataaac gataccacca tcagagcaaa ttggaatata aatacctgtt ttttcaaagt
    14041 attcgtctct agcttgagca acttcaatta tagaagtagc ttgtccacgt cctatacctt
    14101 tttgttcacg agttatacat atagaaccac caccaatacc aactttaacg aagtcagcac
    14161 cagcttcagc taggtaaagg aatccttcgc ggtcaactac atttccagct cctaccttaa
    14221 ctgtatcacc atatttttca cgaatgaaat caagtgttat tttttgccac tcagaaaaac
    14281 cttctgaaga atctatacat aaaacatcag caccagcttc aactaaagca ggaacacgtt
    14341 cagcaaagtc tctagtatta ataccagctc caactacata tctcttagaa gaatctaaaa
    14401 gttcaagtgg attttcttta tgagaagagt aatctttacg gaataccata tacataagat
    14461 taccattatc atcaagtaca ggaagagaat taagtttgtg gtcccatata atattgtttg
    14521 cttcctttaa agtaacatct ttgttagcat aaactataga tgacatagga gtcataaatt
    14581 cactaacttt agtatctaaa tccatacgac ttatacgata atcacgactt gcaacaatac
    14641 ccaataattt accagttgaa gttccatctt cagtaacagc tactgtagaa tgtccagttt
    14701 tttcttttaa gtcaagtatg tcttttaatg tgttatcagg tttaatattt gaatcactta
    14761 caacaaaacc agctttgtgg gatttaacac gtgctaccat agcagcttca ttttcaatag
    14821 tttgagaacc gtatataaat gaaataccac cttctttagc aagtgcaata gccattttgt
    14881 catcagaaac agattgcata atagcagaag tcaaaggtat attcatataa atatctgctt
    14941 cctcgccctt tttaaattta gtaacaggtg tttttaaact tacattagca ggtatacaat
    15001 cagtagaaga gaaacctggt acaagaaggt attcactgaa agtatgtgat ggtgtatcaa
    15061 aataaaacgc cattttttaa tcactcctta tatttttata ctttttataa ttgatagtat
    15121 taattataac ttattcttta attttattaa agtattttgt caaatattga gatttttgaa
    15181 aaatttaaac tattttaatt
//
LOCUS       DAYAOY010000101        15037 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYAOY010000101 DAYAOY010000000
VERSION     DAYAOY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.101"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..225)
                     /locus_tag="ACGI6B_003180"
     CDS             complement(<1..225)
                     /locus_tag="ACGI6B_003180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752596.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKT"
     gene            346..516
                     /locus_tag="ACGI6B_003181"
     CDS             346..516
                     /locus_tag="ACGI6B_003181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752597.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            519..1157
                     /locus_tag="ACGI6B_003182"
     CDS             519..1157
                     /locus_tag="ACGI6B_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9752598.1"
                     /translation="MNNLQIFEKLEFGQIRMAEIDNKPYFVGKDVAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPSIRET
                     GQYQAQQHVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIRVIKSERPYKQLSYFEN"
     gene            1216..1386
                     /locus_tag="ACGI6B_003183"
     CDS             1216..1386
                     /locus_tag="ACGI6B_003183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752599.1"
                     /translation="MKNNISDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSTPAK"
     gene            complement(1389..1622)
                     /locus_tag="ACGI6B_003184"
     CDS             complement(1389..1622)
                     /locus_tag="ACGI6B_003184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752600.1"
                     /translation="MNIGKITKIEATCFDCGTKVTITESKFREVCDNGLPCPMCKEHI
                     SNSNFIAEQALQYNHAVNKLEKELDSCKNIHIY"
     gene            1691..1888
                     /locus_tag="ACGI6B_003185"
     CDS             1691..1888
                     /locus_tag="ACGI6B_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752601.1"
                     /translation="MAINDNINKILRDRNLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            1927..2133
                     /locus_tag="ACGI6B_003186"
     CDS             1927..2133
                     /locus_tag="ACGI6B_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752602.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            2305..2493
                     /locus_tag="ACGI6B_003187"
     CDS             2305..2493
                     /locus_tag="ACGI6B_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752603.1"
                     /translation="MRIIYKNKIYKVEQDKVLFRITYYDEQKNNRKFNNNKKVKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            2509..2721
                     /locus_tag="ACGI6B_003188"
     CDS             2509..2721
                     /locus_tag="ACGI6B_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752604.1"
                     /translation="MVFNLEKFKVGNAIRISCERFGFEIDCIVVVATEEELNLAYFDK
                     ERGCIEYQALMPEDLRYDDYILERLG"
     gene            2737..2907
                     /locus_tag="ACGI6B_003189"
     CDS             2737..2907
                     /locus_tag="ACGI6B_003189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752605.1"
                     /translation="METAISIAKGQIEGANESIKELKSKENYDKESLRWWEGVKQASE
                     NILEFLEIENKA"
     gene            2989..4941
                     /locus_tag="ACGI6B_003190"
     CDS             2989..4941
                     /locus_tag="ACGI6B_003190"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9752606.1"
                     /translation="MKAILLKSLNIENFKGIKELRIDFNNITNVFGENATGKTSIFDA
                     FTWVMFDKDSKNRSVFEIKPLDKQNKVIRGLVTTVTAVLEVNNKEIKLTKKYEEKWTR
                     KRGESEATFTKNETTYMINDTPIKKSEYVKEVAEIANEEQFKLLTNPYFFSNELNWKK
                     GRELILNVCGDITTEQIIESNKDLTLLITEFEKENNIDKIIKNRKAIKNNLSKEKEEI
                     PVRVNECNKSMYDIDFEEIEAQLKAKKADLEAIEDMLLSGTKANEQILKDKEKIFELK
                     QETQSIKQSASEKGNKKRNELLKEKDELKYNIKNLRNNLVYLERDNELKETLKKRAIE
                     KTTNLRDKWTKKSQETLDLSVIQTECPTCKRPLDLEDIEEKKKEMLDNFNLNKAKELK
                     EIAELGKSKNDDVELFNTEIERLKVDINKTLEEIQEKAVLLDKIKKELESTKSTEIYS
                     ADEEKRLTEISAEIKELEEKINNKDADKNIDELKENKKKLTSEIELLNKELAKRDINK
                     ELLDRKEQLLEKEKALGIELAHQEKILNLCELFIKTKVSLLESNISSKFKNVTFKLFK
                     EQINGGIEETCEALINGVPFSNANTAGQINGGLDIINTLSNHFEVKMPIFVDNRESVN
                     NLIDIDSQVINLIVSNDNPLKIEGVN"
     gene            4942..5859
                     /locus_tag="ACGI6B_003191"
     CDS             4942..5859
                     /locus_tag="ACGI6B_003191"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFG9752607.1"
                     /translation="MAERNITDVVLNRVNELKDSGELVIPPTYSAVNALKSAYLILQE
                     TLDKSYKPVLETCTPISIINSLLNMVIQGLSPAKKQCYFVAYGNKLQLMRSYMGTIAV
                     TKRLSNIKDIKAYCIYEGDKFKVKYNKENAVLEIGEYEPAFENIDIGKIKGAFAVIVG
                     NEGILHTEIMTFTQIERAWLQGVGYSNPKNKVHDNFTEEMAKKTVINRACKMYANTSD
                     DSDLLIGAFNETDRVITSEEIAKDTDKEVEKEISQNANIKSLEMPSQVDNTIFTQTKE
                     KVAINIDEDKPNLAEFEEAIQVELDTPPF"
     gene            5862..6572
                     /locus_tag="ACGI6B_003192"
     CDS             5862..6572
                     /locus_tag="ACGI6B_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9752608.1"
                     /translation="MIKILGSSSRGNCYLLQLENETLILECGVSYKEILKGLDFSLEN
                     VVGCLVTHEHKDHSKAITELTNNGIDVYSSKGTLEALKIENHRTKIIESEKLFKIGNF
                     KIVPFNTKHDAVEPLGFLINHSSFGNLLFITDSYYCEYNFNNLNHIMIECNYSKDLLD
                     NNKDKIYLRNRIVKSHFELSNVINFLKVNDLSNIKTITLLHLSEDNSDKDLFIREIEK
                     NVGVPVIVAEKGLEIYLD"
     gene            6583..7476
                     /locus_tag="ACGI6B_003193"
     CDS             6583..7476
                     /locus_tag="ACGI6B_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFG9752609.1"
                     /translation="MAKYRILQANFWDDGFVLDLTPEEKYFYNYLLTNGRASQCGCYE
                     LPYKIMEMQTGYNRETVEKLIKRFIEYGKIKYDSTTKEILIINWSKHNFSKSPKVRTC
                     ILKEVESIKNKEFQQYMYRVCIDYGYPIDTVSIDYGEKEKEKQKEKEKQKEKEKQKEK
                     VEPLICEEQEKNELKEFKKLYEENIGVVYPVTAEWLIEISSDVDIRLFKSAIEICAEK
                     MNMNIAYLKGILKKWKDANITTYEQLESYRLQQENKKAKKVVNGQLSKNKFANFEQTF
                     TKYTENELDNIIKKSQDAKFK"
     gene            7534..7881
                     /locus_tag="ACGI6B_003194"
     CDS             7534..7881
                     /locus_tag="ACGI6B_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752610.1"
                     /translation="MGKIWMDAGTFLETKDIGDIECNLREMINKNKQKNLIGRINSKG
                     QGRKSKKVECTNIITGESKIFVSAVEASEYLYFTELHITRLARTGKTTKNGWKVRYIE
                     EVSNGISKCGTSN"
     gene            7850..7987
                     /locus_tag="ACGI6B_003195"
     CDS             7850..7987
                     /locus_tag="ACGI6B_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752611.1"
                     /translation="MVLANVEQVIKLAEKILSKKKCSVNKAIDIAIKILNRYEYEGMI
                     K"
     gene            7984..8433
                     /locus_tag="ACGI6B_003196"
     CDS             7984..8433
                     /locus_tag="ACGI6B_003196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752612.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWRKVC
                     NVGICSEEWAGNNQEIFEGDILKENYNPHNRSSYGIVKRDFNSIKLYLEWHYLKKFEG
                     EWIEIIDKTEIYHSRGYKVIGNEYENLEEVRKEFLERKEMLENERLS"
     gene            8417..8542
                     /locus_tag="ACGI6B_003197"
     CDS             8417..8542
                     /locus_tag="ACGI6B_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752613.1"
                     /translation="MNVLASLILIVGSFIAGRVYEYRLNLKEFENYDNIGGFKNE"
     gene            8535..8693
                     /locus_tag="ACGI6B_003198"
     CDS             8535..8693
                     /locus_tag="ACGI6B_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752614.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAEHDRVRILDLETKEKY
                     DLLRSEGD"
     gene            8697..9020
                     /locus_tag="ACGI6B_003199"
     CDS             8697..9020
                     /locus_tag="ACGI6B_003199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752615.1"
                     /translation="MSKVIQCDFCKEIFEKYNGECIELYKKDCSKGVLGIDKHICPTC
                     YEKFIEGKIEKVENAKRFKDKLIDFLVEHEVCDCCACDCVFDSNDEKKCKAGIREWVE
                     SEVEE"
     gene            9022..9321
                     /locus_tag="ACGI6B_003200"
     CDS             9022..9321
                     /locus_tag="ACGI6B_003200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752616.1"
                     /translation="MAKLIVSIPLTKYLKDITKEWGETYITKTYGGHVYLSDEAPGEV
                     FKKEDDLNYIEFNDSDIVMRLPKEVHQHINLKKGCNKNLKDVLKEIKKIEEKTNI"
     gene            9429..10325
                     /locus_tag="ACGI6B_003201"
     CDS             9429..10325
                     /locus_tag="ACGI6B_003201"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFG9752617.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFKGRRSSLFFAVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLIELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFVVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTSGVLVNAVLTPDRVNKRQN
                     GRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQ
                     LYKQAGNAVTANVVYEIAKRMG"
     gene            10404..10625
                     /locus_tag="ACGI6B_003202"
     CDS             10404..10625
                     /locus_tag="ACGI6B_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752618.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            10622..11626
                     /locus_tag="ACGI6B_003203"
     CDS             10622..11626
                     /locus_tag="ACGI6B_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFG9752619.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTVVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNAILESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSISFEEDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTEFLNATKVKDDKYRTKKFKDTVENWITNVL
                     GNDIKQAEDIRSILNYTLKEKHEIDIKDFVDKSIKDDELKNSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVNFYNEK
                     "
     gene            11637..11927
                     /locus_tag="ACGI6B_003204"
     CDS             11637..11927
                     /locus_tag="ACGI6B_003204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752620.1"
                     /translation="MENKDNFVLNKLGPASKVMKKVEIDEKEIKSYLKELKECEEVKV
                     FIIRKDGIEIPIVFKDGNMNEWLRKEIEISFDRFKDEIAQVKKALIKVTGEN"
     gene            11947..12114
                     /locus_tag="ACGI6B_003205"
     CDS             11947..12114
                     /locus_tag="ACGI6B_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752621.1"
                     /translation="MTNKEMCKSNNLDEREVCKEFGKEICGSCINDRVDCESKDCDIA
                     YENWLEKNTER"
     gene            12200..12541
                     /locus_tag="ACGI6B_003206"
     CDS             12200..12541
                     /locus_tag="ACGI6B_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HFG9752622.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNLKVCESYIEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELIK
                     INSHLELEDLN"
     gene            12667..13074
                     /locus_tag="ACGI6B_003207"
     CDS             12667..13074
                     /locus_tag="ACGI6B_003207"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9752623.1"
                     /translation="MKVFLVIDGEPVGKERPRMNTYAKRTYTPNKTKNYEDLIKWLYQ
                     SKVKYYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVIKVVADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            13176..14030
                     /locus_tag="ACGI6B_003208"
     CDS             13176..14030
                     /locus_tag="ACGI6B_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFG9752624.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRELKHINELINNNIKRFKTNIDIIDLKVSRSERLGDLKANPFEGLGYEDVGYSKQS
                     FNQSRNIYLLSERGYSKLLKILEDDKAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEVKRVATKSLGGHGSKA
                     YKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEEEIRLL
                     NSQLSIVS"
     gene            14182..14688
                     /locus_tag="ACGI6B_003209"
     CDS             14182..14688
                     /locus_tag="ACGI6B_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752625.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSANIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIVHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
BASE COUNT         6399 a         1441 c         2899 g         4298 t
ORIGIN      
        1 tgttttctct agcaactctt ttaggacacc aggaatccta agagtagaac gaacagtatc
       61 atcactttta tacgagattg attgaagttc attaactttt gaatttctaa taatatcatt
      121 aatagcgtat agaataagcg aagatttgag aatgcctgtt tgataagaaa ttttttctaa
      181 attttcattt aatggtgtat atattcttac agtaatagta gtcattttga cacctcccta
      241 atgacattat acaaaaaata tttaaaaaag tcttgacact aaaatagtgt cataatctaa
      301 tgattataga cagtaaaaca gtgtcataag gagggaggaa taagaatgga aaaagtaaga
      361 atgacagtaa ggctcgtccc aaaattaaat caatatgtac agaatatagc caaagaatgt
      421 ggaaaaagta aaaattctat tattgtggat gcatgttggg agtttattga gaaaataaaa
      481 aaagaaaata aattcaatgt agaaagcgag gaataaatat gaataatcta cagatatttg
      541 aaaaattgga atttggacaa ataagaatgg cagagattga taataagcct tactttgtag
      601 gaaaagatgt tgcaaagtca ttaggttata aaaatactaa tgatgcaatt ttaagacatt
      661 gcaaaggggt agtgaaacac gaggggttta aaattaatgg tattaagatt gctttgataa
      721 ctgagggtga tgtttataga ttaatagtag gttcaaatct accaaatgca gaaaagtttg
      781 agagttgggt gtttgatgaa gtgcttccaa gcataagaga aacaggtcaa taccaagcac
      841 aacagcatgt aataacagaa cttacaggaa ctataggaga cttaaaagga acaataaaag
      901 aatataaaaa gttgtgtaag ataacatgct ctaagaaaca acagtattct aaatacataa
      961 agaatagact ggatatagac aaagctaata aagaatacaa ccaagtgaaa gcaaggctat
     1021 tcctaattct tggagttgag aagtgggaag acatagactt cgatacatct aataatttaa
     1081 tacaaattat agatgattgt ataagagtta taaagtcaga gagaccatac aagcagttga
     1141 gttattttga aaactaaata cagaatattt tgaaatatat tgttttaatt aattattaac
     1201 taggaggtta aatacatgaa aaataatata agcgattcaa gagtaaaata tttttgcaag
     1261 tgtccatact gtgggtttga taatgaggta gaagttaaaa aaggattgaa gcctaaaata
     1321 tgttgcatat gtacaaaaga agttgagtat gaaaaactgg agcaacaaag tactccagca
     1381 aaataaaatt aataaatatg aatattttta caagaatcta attctttttc taacttatta
     1441 acagcatggt tatattgcag tgcctgttct gctataaagt tagaatttga tatgtgttct
     1501 ttacacatag ggcatggtag cccattatca caaacttccc taaatttaga ttctgttatg
     1561 gtaacttttg taccacaatc gaaacaagta gcttcaattt tagttatttt accgatattc
     1621 ataaatcacc acctaatcat atatttatag gatttatcct acaaatatag tatatcaaag
     1681 gagggaaata atggcaatta atgacaacat aaataaaatt ttaagagata gaaatttaaa
     1741 agcgtggaaa ttagcaaaag aaataggcgt agattcagga aatttatatg caattttaag
     1801 aggagaaaat aaaaatccaa ctatagatac attgataaaa ctagctgact atttagatgt
     1861 tacattagac gaattagttg gaagatagaa attaaataca gaatattttg aaagtaggtg
     1921 tattgtatgg caaaagcagt agctaaagaa caattgtttt atagggcaaa agatatagct
     1981 aagattttag atgtatgtga agcaaccgct tataaaataa ttggagaatt aaataaagaa
     2041 ttagaaaaag aaggttggaa gacctttcct ggaagggtat cagtaactta tttcaaagaa
     2101 agatattgtt ataaaacaaa aaaaggggca taaaaaatgc tatgagagta gctaaagata
     2161 tgtttcctaa agtaattgta taaagaggaa gggggtgatt tagttgaatg taagagtact
     2221 gatagcttat atacagtttt gtaagcaata caataagaaa gcgagttttg aaggtcttaa
     2281 aaaatacaac aaaggggtaa ttgcatgaga ataatttata aaaacaaaat ctacaaagta
     2341 gaacaagaca aagtgttatt tagaattaca tactatgatg agcagaaaaa taacaggaag
     2401 tttaataaca ataagaaagt aaaaagaagt gttttaacaa gagatataga gttagttaac
     2461 ttgtatttac cagcacattt aaaaataaag taggaggttt aattgcatat ggtatttaat
     2521 ttagagaagt tcaaagtggg aaatgcgata agaataagtt gtgaaagatt tggttttgaa
     2581 attgattgta ttgtagtagt agcaactgag gaggaactaa atttagctta ctttgataaa
     2641 gaaagaggtt gcatagagta tcaagcattg atgccagaag accttagata tgatgattat
     2701 attctcgaaa gattaggtta gagggggtgt attttaatgg aaacagctat atctattgca
     2761 aaaggtcaaa tagaaggagc gaatgagagt ataaaggaat taaaatcaaa agaaaattac
     2821 gataaagaaa gtttaagatg gtgggaaggt gttaagcaag ctagtgaaaa tatactagag
     2881 tttttagaaa tagaaaataa ggcataagaa aagagccatt acgattggct caattcaaaa
     2941 acataataaa atttaattag ctatattata gcacaagggg gaactaaaat gaaagcaatt
     3001 ttattaaaaa gtttaaatat agagaatttc aaagggataa aagaacttcg catagatttt
     3061 aacaacataa ctaatgtgtt tggagaaaat gcaacaggta aaacaagtat atttgatgcc
     3121 tttacgtggg taatgtttga caaggatagc aagaatagaa gcgtatttga gataaagcct
     3181 ttagacaagc aaaataaagt cattagagga cttgtaacaa ctgtaacagc agtgctggaa
     3241 gttaataata aagagataaa gttaacaaag aaatatgaag aaaaatggac tagaaaaaga
     3301 ggagaatcag aagcaacttt tactaaaaac gaaacaacat acatgataaa tgatactcca
     3361 ataaaaaaga gtgaatatgt aaaagaggta gctgaaatag caaacgagga acagtttaaa
     3421 ttacttacta atccgtattt cttttcgaat gagcttaact ggaaaaaagg tagagaatta
     3481 attctaaatg tgtgtggaga tataacgaca gaacaaatta tagaatcaaa taaagattta
     3541 actttactta ttacagagtt tgaaaaggaa aataacatag ataagattat taaaaataga
     3601 aaagctatta aaaataactt atctaaagaa aaagaagaaa taccagttcg tgtaaatgag
     3661 tgcaataaaa gcatgtatga catagatttt gaggaaatag aggctcaatt aaaagctaaa
     3721 aaggctgact tagaagctat tgaggacatg ttactaagtg gcacaaaggc gaatgaacag
     3781 attttaaaag ataaagaaaa gatatttgag ctaaaacaag agacacaaag tattaaacaa
     3841 tcagcaagtg aaaaaggaaa taagaaaagg aatgagctgt taaaagaaaa ggatgagttg
     3901 aaatataata ttaaaaactt aagaaataat cttgtttatt tagaaaggga taatgagtta
     3961 aaagaaactt tgaaaaaaag agcgattgaa aaaacaacaa atttaagaga taaatggact
     4021 aaaaagagtc aggaaacatt agatttaagt gttatccaga cagaatgccc aacttgtaaa
     4081 agacctcttg atttagaaga tatagaagaa aagaaaaagg aaatgttaga taattttaat
     4141 ctaaataaag ctaaagaatt aaaagaaata gcagagctag ggaaatctaa aaatgatgat
     4201 gtagaattat ttaatacaga aattgaaaga ttaaaagtag atataaataa gactttagaa
     4261 gagatacaag aaaaagcagt attattagat aaaataaaaa aagaattaga aagtacaaaa
     4321 tctacagaga tatattcagc agatgaggaa aaaagattaa cagaaattag tgcagaaata
     4381 aaagagttag aagaaaaaat aaacaataaa gatgctgata aaaatataga tgagctaaaa
     4441 gaaaataaga aaaaactgac tagtgaaatt gagttactta ataaagaact agctaaaaga
     4501 gatattaaca aagagttatt ggatagaaaa gagcagttgt tagaaaaaga aaaagcgtta
     4561 ggtatagaac tagctcatca agagaaaata ctcaacttat gtgaattatt tataaaaact
     4621 aaagttagtt tattagaaag taatataagt agtaaattta agaatgtaac atttaaacta
     4681 tttaaagagc agataaatgg tggtatcgaa gaaacttgtg aagctttaat aaacggtgta
     4741 cctttcagca atgccaacac agcggggcaa ataaacggag ggctggatat aataaatact
     4801 ttatctaatc actttgaagt taaaatgcca atattcgtcg acaacaggga gagtgtgaat
     4861 aatttgattg atattgatag ccaagtgata aatttgattg taagtaatga taacccatta
     4921 aaaatagaag gggtgaacta aatggcagaa agaaatataa ctgatgtagt tttaaataga
     4981 gtgaatgagc taaaagatag tggtgagtta gtaattccac ccacttattc agcagtaaat
     5041 gctttaaaat ctgcatactt aattttacaa gaaacacttg ataagagcta taagcctgta
     5101 cttgaaacat gtactcctat tagtataatc aattctcttt tgaacatggt catacaagga
     5161 ttaagcccag ctaaaaaaca atgttacttt gttgcttatg ggaacaaatt gcaacttatg
     5221 agaagttata tgggtactat agcagttact aaaagacttt ctaatataaa agatataaaa
     5281 gcgtattgca tctatgaggg tgataaattc aaagtcaaat acaataaaga aaatgctgta
     5341 ctagaaatag gagaatatga accagctttt gaaaacatag atataggcaa aataaaaggt
     5401 gcttttgctg taattgttgg taatgaagga atcttgcata ccgaaataat gacctttaca
     5461 caaattgaaa gagcatggtt acaaggtgtt ggctatagta atcccaaaaa taaagtacat
     5521 gataatttta cagaagaaat ggcaaagaaa acagtaataa atagagcgtg caagatgtat
     5581 gcaaatacca gtgatgatag tgatttatta ataggtgctt ttaatgaaac tgatagagta
     5641 atcactagcg aagaaattgc taaagataca gacaaagaag tagaaaaaga gatttctcaa
     5701 aatgctaata tcaagtcttt agaaatgcct tcacaagtgg ataatacaat ctttactcaa
     5761 actaaagaaa aagtagctat taacatagat gaagataagc cgaatctagc tgaatttgaa
     5821 gaagcaatcc aagttgaatt ggatacacca ccattttaaa tatgattaag atattaggga
     5881 gcagtagcag aggtaattgc tacttgctcc aactggaaaa cgagacatta attttagagt
     5941 gtggagtcag ttacaaagag atattaaaag gcttagattt cagtttagaa aatgttgtag
     6001 gatgtttggt cacacatgaa cataaagacc actctaaagc tatcacagag cttacaaata
     6061 atggaataga tgtatattct agcaagggta cattagaagc tttaaaaata gaaaatcata
     6121 gaactaagat tatagaaagc gagaaattat tcaagatagg taactttaag atagtgccat
     6181 ttaatactaa gcatgatgcg gtagagccat taggattttt aataaatcat agtagttttg
     6241 gtaacctatt atttataact gatagttatt attgtgaata caactttaat aatttaaatc
     6301 acatcatgat tgagtgtaac tatagtaaag atttattaga taacaacaaa gataagattt
     6361 atttgagaaa tagaatagtc aaaagtcatt ttgaattaag taatgtgatt aattttctaa
     6421 aagtaaatga tttaagtaat ataaaaacaa ttacattgct tcatctaagt gaagataaca
     6481 gtgataaaga tttatttata agagagatag aaaagaatgt gggagtacca gtaatagtag
     6541 ctgaaaaagg attagaaatt tatttagact agaggtgata aagtggcaaa atatagaatt
     6601 ttacaagcta acttttggga tgatggattt gtattagatt taactcctga agaaaagtac
     6661 ttttataact atcttttaac taatggtaga gcaagtcaat gtgggtgtta tgaattaccc
     6721 tataaaatta tggaaatgca aacaggatat aacagggaaa ctgtagagaa gttaattaaa
     6781 aggtttatag agtatggaaa aataaaatat gactctacta ctaaagagat attaataatt
     6841 aattggagta aacataattt ttctaaaagt ccaaaagtac gtacttgtat cttaaaagag
     6901 gttgaatcaa ttaaaaataa agaatttcag cagtatatgt atagagtatg tatagactat
     6961 ggatacccta tcgatacagt atctatagac tatggggaaa aagaaaaaga aaaacaaaaa
     7021 gaaaaagaaa aacaaaaaga aaaagaaaaa caaaaagaaa aagtagaacc gttaatttgt
     7081 gaagaacagg aaaaaaatga gttaaaagaa tttaagaagc tatatgaaga aaatatagga
     7141 gtggtatatc cagttacagc agaatggtta atagaaattt ctagtgatgt agatataaga
     7201 ttatttaaat ctgctataga gatatgtgct gaaaagatga atatgaatat agcatactta
     7261 aaaggtatcc ttaaaaagtg gaaggatgcg aatataacta catatgaaca actagaatca
     7321 tatagattac agcaagaaaa taagaaagca aaaaaagtag ttaatggcca actaagtaaa
     7381 aataagtttg ccaactttga acaaacattt actaagtata cagagaatga gcttgataac
     7441 ataattaaga aaagtcaaga tgctaagttc aaataaaatt aaacttctag gaagtaaata
     7501 tcaatatatt gcttcctaga aagggggtag aatatgggta aaatttggat ggatgcagga
     7561 acatttttgg aaactaaaga tataggagat attgaatgta atctaagaga aatgataaat
     7621 aaaaataaac aaaaaaattt aattggtaga ataaattcca aaggacaagg aagaaaaagt
     7681 aagaaagtag agtgtactaa catcattact ggagagagta aaatatttgt cagtgctgta
     7741 gaagcaagtg aatatttata tttcacggaa cttcatatta ctcgtctagc aagaacagga
     7801 aagactacta aaaatggttg gaaagttaga tatattgaag aggtgtcaaa tggtattagc
     7861 aaatgtggaa caagtaatta agttagctga aaagatatta agtaagaaaa agtgttctgt
     7921 taataaagct attgatatag ctataaaaat attaaataga tacgaatatg aggggatgat
     7981 taaatgagtc taattgaata tagaggttat gattttgaaa ataaaaagtg gatttattct
     8041 aaaacaataa tgtggagtaa tgcagtagac tgtttattaa tgctgaaaga agattttaaa
     8101 tggcgaaaag tttgtaatgt tggaatatgt tctgaagaat gggctggaaa taatcaagaa
     8161 atttttgaag gggatatatt aaaagaaaat tataatcctc acaatagaag ttcatatgga
     8221 attgtaaaaa gagattttaa tagtattaag ttatatttgg agtggcatta tttaaagaag
     8281 tttgaaggag aatggataga gattatagat aaaacagaaa tatatcatag tagaggttac
     8341 aaagtaattg gtaatgaata tgagaactta gaggaagtta ggaaagagtt tttagaacgc
     8401 aaggagatgt tggaaaatga acgtcttagc tagtttaata cttatagtag gaagttttat
     8461 agctggtagg gtttatgagt atagattgaa tctaaaagag tttgaaaatt atgataatat
     8521 aggaggtttt aagaatgaat gatagattag aaatgataaa tgcttctgta aattatatac
     8581 agatgatatg tgaaagttca aatatagcta ttatagcaga gcatgaccga gttagaatat
     8641 tggatttaga aactaaagaa aagtatgacc tattaagaag tgagggggat taaaaaatga
     8701 gtaaagttat acaatgtgat ttctgtaagg aaatatttga aaaatataat ggagaatgta
     8761 ttgagctata taaaaaggat tgtagcaaag gggtgcttgg gatagataaa cacatatgcc
     8821 caacttgtta tgaaaaattc attgaaggaa aaatagagaa agtagaaaat gctaaaagat
     8881 ttaaggataa attaatagac ttcttggttg agcatgaggt ttgtgactgt tgtgcttgcg
     8941 actgtgtgtt tgattctaat gacgaaaaaa aatgcaaggc aggaattaga gagtgggttg
     9001 aaagtgaggt agaagaataa tatggcaaaa ttaatagtta gcataccttt aactaaatat
     9061 ttaaaagata taactaaaga atggggagaa acttatataa ctaaaacata tggtgggcat
     9121 gtctatttaa gtgatgaagc tccaggagaa gtatttaaaa aagaagatga tttaaattat
     9181 atagagttta acgattctga tattgtcatg agattaccta aagaagtaca tcaacatata
     9241 aatttgaaaa aaggttgcaa caagaatctt aaagatgtat taaaagaaat aaaaaagata
     9301 gaagagaaaa caaatatata gaaaatatct aattaaaaca gtttagagag ttgcaaaata
     9361 tcttttagta taaatttatt gttgaagtgt tttgtgactc tcaaaaatga aaataaggag
     9421 gagctatatt gcttacattt ttagatttat tcgcagggat aggtggtttt aggctaggaa
     9481 tggaaaaagc aggacataaa tgtttgggac attgcgaata tgataaattc gcaaatttaa
     9541 gttataatgc catgcacaaa ccgaaggagg atgaatggtt tgaaagagat attagagaaa
     9601 ttagaacaga aaatatccca agagcagatg tctggtgttt tggattccca tgtcaagaca
     9661 tttctgttgc aggtaaacaa tttggattta aaggaagacg ttcaagttta tttttcgcag
     9721 ttacaaaact tattagagaa ctcaaagaag aagatagacc caagtattta cttattgaaa
     9781 acgttaaaaa tctacttagt gttaatggag gatttgattt cctcaaagtt ctcattgaac
     9841 tggatgaaat cggctatgat gcagagtggc aagttcttaa ctctaaaaac ttcggagtac
     9901 cccaaaatag agaacgaata ttcgttgttg gacattttag aggacgaagt acacgaaaag
     9961 tatttcctat cgaaagaaaa agtagaaaaa atcttgaaca actaaataat ccaactcata
    10021 gtacaagtgg agttttagtc aatgcagttt taacacccga tagggtaaac aaaagacaaa
    10081 atggtcgtag aattaaagaa agcggagaac caatgttcac actaacagct caagataaac
    10141 atggaatttt gaaaaatgga gatataagaa ggttaacacc aaaggaatgc tttaggttgc
    10201 aaggatttcc ggataaatat tacgaaagag cagcaagtgt atgctcagat agtcaactgt
    10261 acaaacaagc gggaaatgct gttactgcaa atgttgtata tgaaatagca aaaagaatgg
    10321 gctaagagtt gcaaaatgtc ttttagtatg agtattgttg aagtgttttg taactctcaa
    10381 aaatgaaaat aaggggtgaa taaatgtcaa agtatgtgct tagatggcaa aaagggttat
    10441 tgttggatga acgtaagata aattattcat gtggaagtaa agaaatgcta aaacaaaaag
    10501 cagaattatt agctaaagat gacaagatat tgcttgtaac aatagataaa gttgaagaag
    10561 ttataaaaga tactagaagt caaaaaatgg ctgaatatta ttgtgatgga ggaattgaaa
    10621 tatgataata cacaaattta taatacatgt tttagataag aatagcgata caccaatact
    10681 aaatgatttt gagggtaggg ttagtcaaga tattgaagct ttctttcaaa agaaaataag
    10741 caaagtatca agagataatg acatcagaac agtagtattt aatgactata gtaacaatct
    10801 aattaagaag tgttgtgaac aaattattta tgatgaaagt tcatttttaa ataactctaa
    10861 agagattgca gcttatttat ttgatgttat gaaattgaat gctatattag aatcttgcga
    10921 cttagcaatt tgcttatact ctcaaaaaga tgaaaagaaa gttgctatat taaagcttga
    10981 ttacaataat tcgtatactc attctattag ctttgaagag gataaattta atatacagat
    11041 gtctaagaat gaaattaata tacaagagac taagacggtt aaaattgctg ctttggttgg
    11101 attgagtgga atgaatgacg aatatcatct aaaagtattg gataaggatg cagagaagga
    11161 agaagctaat tctaagtttg ttacagagtt tctaaatgct actaaagtga aagatgacaa
    11221 gtataggact aaaaagttta aagatacagt tgaaaattgg ataactaatg ttcttggaaa
    11281 tgatataaaa caagctgagg acataagaag tatattaaat tatactttga aagaaaagca
    11341 tgaaattgat ataaaagatt ttgttgataa atcaattaaa gatgatgagt taaaaaatag
    11401 ttttaaagaa catatggaag aaaagggtct tgatgaaagt tttagcatag ataaaaaatg
    11461 ggttgaaaaa aagcttaaaa agagaaatat aaaaactgac aatggttttg aaataaaagg
    11521 taacttaact gattttgagg acccaatgaa atatacagta agacaaaatc aaaatgggtc
    11581 tatagatata attattaaga atgttaattt ctataatgaa aaataagggg gctacaatgg
    11641 aaaataaaga taattttgta ttaaataagc ttggaccagc aagtaaagta atgaaaaaag
    11701 ttgaaataga tgaaaaagaa attaagagtt atctaaaaga actaaaagaa tgtgaagaag
    11761 taaaagtttt tattattaga aaagatggaa ttgaaattcc aatagttttc aaagatggaa
    11821 atatgaacga atggttaaga aaagaaattg agataagttt tgatagattt aaggatgaaa
    11881 ttgctcaagt taaaaaagca ttgattaaag ttacaggaga gaattaagaa gttagaaggt
    11941 gctcatatga ctaataaaga aatgtgcaag tcaaataatc ttgatgaaag agaagtgtgt
    12001 aaggaatttg gaaaagagat ttgtggtagt tgtataaatg atagggtaga ttgcgaaagc
    12061 aaagattgtg atatagcata tgaaaattgg ctagagaaga atacagaaag ataattataa
    12121 aaataaagtc aaggtaagtt tgtgaatgaa actagaatgt tatagactta ctttgactta
    12181 taaaaaagga gtgtattaaa tggctaatat atattgcgaa aattataatt gtaaaaacta
    12241 ctttgaagat atgtgtatgc ttgaaagaat tgaaattaat aacctgaaag tgtgtgaaag
    12301 ttacattgaa ggtaaaaatg agctatataa attagaaaac ggatatacta tacatcctaa
    12361 agatttgaaa atggtgaaaa gtaaagatta ttctgttgaa gttactcata ttccaactgg
    12421 tattacagta aaatgccgtt ctacaaatag tattttaaaa aataaaaata agtgtttgga
    12481 agttctagaa gaagaattaa taaaaataaa ctctcactta gagctagaag atttaaacta
    12541 aataggaagt gagcttatga aacgaagaag atgcagttgg tgtggtaagc tattttatct
    12601 taaggaaaaa tctaaggatg tttattgttg taaagaatgt aggaagaagg ctaagaaggt
    12661 gaaaaaatga aagtttttct tgtaatagat ggagagccag ttggcaagga aagaccaaga
    12721 atgaatactt acgctaaaag gacctataca cctaataaga ctaagaatta tgaggaccta
    12781 ataaaatggc tatatcaatc taaagttaaa tattactttg aaggttatat aaaaatgact
    12841 ttaagatgtt attattctat agctaaaagt aacagtaaaa aggttaagga gcagaaaaga
    12901 aataatgtgt taagacctag taaaaagcct gacattgata atgtcattaa agtagtagct
    12961 gattcactca atgagatagc ttataaggat gatacacaga ttgttgaggt tgtagctagt
    13021 aaatattaca gtgataaacc aagagttgag gttatattag aagatgttat ttaaccaacg
    13081 gaaaaatccg ttcgttagat taggtactca gtttttcata aaaatgtgaa aaagctgaat
    13141 agagaatata tcaaatggta aaggagagat aaattatgaa tgaaaatata aataaagaaa
    13201 taacagtact tggaacttta gaaatcgagg gaatgaaatt tcataacatt gagggtggat
    13261 ttggagaaca taagaaagca atgctagtaa aagatatagc tgagatacat aatagagaac
    13321 ttaaacatat aaatgagcta attaataata atataaagag atttaaaaca aatatagata
    13381 ttatagattt aaaagtgagt cgttctgagc gactaggtga tttaaaggct aatccttttg
    13441 aaggattagg ttacgaggat gttggatata gtaaacaatc ttttaatcag tcaagaaata
    13501 tttacttgtt atctgaaaga ggttattcaa aactactaaa gatattagaa gatgataagg
    13561 cttgggaaca atacgagaaa atagttgatg gatatttctc tatgagaaaa gagttaaata
    13621 accctctttt aagtgcatcg aaggagttac aggctatatt tatgctagat aagaaacaag
    13681 aagtcttaga aactaaaata gagaatgtta atgagaagtt agagaacttt atggatgatg
    13741 caccattatt caatatcgag tgtgagtgta ttgttaaaga ggttaagaga gtagcaacaa
    13801 aatcacttgg tggacatgga agtaaggctt ataaaaataa atctttaaga ggtaaagttt
    13861 atagtgatat ataccatcag attaaacgag aatttggagt agatagttat aaggctataa
    13921 agcgttgtca gttagataaa gtattagaga ttgtaaataa ttataagtta cctatagtgt
    13981 ttgaagaaga aataagactt ttgaatagtc aattatcaat tgtgagttaa gacaggaata
    14041 gatttttctc ctttaaaggg gaaaacctgc aatagttaat taaaagagaa aaggagtgct
    14101 ttcacactcc acttgtcaaa aatataaaac ttttatatac aagattatta taacataaat
    14161 aattgatagg agtgtgtgag tatgtctaaa actaaaaaag agttttttaa tgcaactaag
    14221 aaacaacttt ctaattataa acaattaagt gcaaatataa taaaattaaa aaatgaaata
    14281 caaatgttga aagataattc ggttggagat ttaatgaaag gtataagtta tgatagtgtc
    14341 aaaacaggaa aaacaaacaa aactagtaac atgattgagg atgctattgt taatgtatca
    14401 gacttaataa cagaaaagga aatagagtta tatgaagcag aaataattaa atccacaata
    14461 gatttagcta taagaaactt aaaacctata cacagacaaa ttattgaact taaatatata
    14521 gatggtctaa tgtggcaaga aatggttgat atagtacatt tagaagaaag acaattaagt
    14581 gtaagagcta gtcaagctat tagctcaata tcaatagcat tatttgggaa gaaagcatta
    14641 atagagcaag aaccactttt tgaattgtta gattacaaac taaattaaaa aagtaagagt
    14701 aattttgagt gctgaaaatg tgcaggtttt tttgttttag acatgagata atagtattgt
    14761 ggaaatgaat atttctctct caaaactaaa tatatatgtg ggctaggtaa agggattcac
    14821 ctagcttata tgaacagact aggcagggcg tgaggacgct gttagttcaa ttctaactat
    14881 gttcaatctt tagtttttct atttcaatta atctacggat acactaaaaa atagtatttg
    14941 aattgagatt aaaatctcat acagttttgt atcttaattc agagtctaaa ccgagtgggg
    15001 cttggtaacc tcactcacca tgcaggtact ggtgttt
//
LOCUS       DAYAOY010000102        15033 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYAOY010000102 DAYAOY010000000
VERSION     DAYAOY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.102"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            318..1151
                     /locus_tag="ACGI6B_003210"
     CDS             318..1151
                     /locus_tag="ACGI6B_003210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9752626.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1220..2176
                     /locus_tag="ACGI6B_003211"
     CDS             1220..2176
                     /locus_tag="ACGI6B_003211"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9752627.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2228..2710
                     /locus_tag="ACGI6B_003212"
     CDS             2228..2710
                     /locus_tag="ACGI6B_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9752628.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2722..3591
                     /locus_tag="ACGI6B_003213"
     CDS             2722..3591
                     /locus_tag="ACGI6B_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9752629.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3634..4389
                     /locus_tag="ACGI6B_003214"
     CDS             3634..4389
                     /locus_tag="ACGI6B_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9752630.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4431..5252
                     /locus_tag="ACGI6B_003215"
     CDS             4431..5252
                     /locus_tag="ACGI6B_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HFG9752631.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5257..6018
                     /locus_tag="ACGI6B_003216"
     CDS             5257..6018
                     /locus_tag="ACGI6B_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFG9752632.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6179..6436
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6549..6788
                     /locus_tag="ACGI6B_003217"
     CDS             6549..6788
                     /locus_tag="ACGI6B_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9752633.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7041..8087
                     /gene="ilvC"
                     /locus_tag="ACGI6B_003218"
     CDS             7041..8087
                     /gene="ilvC"
                     /locus_tag="ACGI6B_003218"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFG9752634.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDYTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDVAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            8140..9828
                     /gene="ilvB"
                     /locus_tag="ACGI6B_003219"
     CDS             8140..9828
                     /gene="ilvB"
                     /locus_tag="ACGI6B_003219"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFG9752635.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKMDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10039..10716
                     /locus_tag="ACGI6B_003220"
     CDS             10039..10716
                     /locus_tag="ACGI6B_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9752636.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10885..12921
                     /locus_tag="ACGI6B_003221"
     CDS             10885..12921
                     /locus_tag="ACGI6B_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFG9752637.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13112..13678)
                     /locus_tag="ACGI6B_003222"
     CDS             complement(13112..13678)
                     /locus_tag="ACGI6B_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9752638.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            13966..14901
                     /locus_tag="ACGI6B_003223"
     CDS             13966..14901
                     /locus_tag="ACGI6B_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HFG9752639.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
BASE COUNT         6209 a         1483 c         2637 g         4704 t
ORIGIN      
        1 tggcaaatag ttgttttgct atttattatt tgtttaatat ttctaattta taaaaaaaaa
       61 attataaatt ttaaaaatta taaatgacat tttaactaga attaattgta aaaaacacta
      121 tataatactc aattttcaac attctataca attatacaga aattatacag cataataatc
      181 accgaaataa caaattttaa aaattaagca aaactttttg tcgaagttca acgtctatat
      241 atatgtagtg taaatttttg ggataaaata ctacaaaaat ataaatgtaa aaatatagta
      301 atattggagg tcgaacgatg agaaataaaa agaaaaaaat aaatagaaag aagttgtatt
      361 tgcttttaag tgctattgta gtgtgtctgg cttttatagt agttggtgtt cgcgtcagct
      421 ctttaaatat gaaggaaaat gaaattagta gaaatgcaaa actgtcaagt tctactatta
      481 cagatatagt atctaataca tcaatagaag ttagtaaagg gccaagtaaa agttctggaa
      541 aaatagcata cataactata gatgatggtc catctaaatt tactgaccag atgataaaaa
      601 ctttaaataa atacaatgtt aaagcaactt tttttatgat agatgggaat atgaaggaat
      661 atccacagca agttaaaaat ataattaaaa atgggaacac agcaggtttt catagtgtat
      721 cacatgatat tcacaagctt tatgttacta gtacatcagc aaaagaggaa tttgatacaa
      781 atgatcaaac tttttataag ataactggta aacattcaaa agtaataaga ataccttatg
      841 gaagcaaacc atatacacca caagcctctt atcaggcttt agttgatgct ggatataaaa
      901 tttgggactg ggacttagat acagaagact ggagatctaa ttcttctcaa attgtacaaa
      961 atgtaaaaaa tcatattaag aatagaaaag gtgaagataa agaccaactt gtagttttaa
     1021 tgcatgagaa aaaacagagt gcagaagctt tagattcagt tttaaaattc ttatcagatg
     1081 aaggttatga atttgcgcca gtagatcaaa accaaatacc aaaaaattat tggttacgta
     1141 atttagagta gttaaaatat aagaatattg attgatattt ttagaaattg agaataaaaa
     1201 tgagtgaggt gtaggtatat tgaataaaaa actttatata ggaatggtcg gaatattgtc
     1261 tttgatgatg gttggttgta ataaaagttt agcaaaagta aatgatgtgg aaatcacaaa
     1321 agaacagtac aaaaaaacaa aagcagtttt atctgctact aataattata taaatgggca
     1381 gtctttagat gaattagaaa aaactttgga taaaaaaggt agaaataaat tagaaaatgt
     1441 tataatatcg tttatggtgg ataatgaact tttatatcaa gaggctaagg ataaaggact
     1501 aactccaagt aaaagtgaag tagactcaaa atatcaagaa ctagaagata agatgaattt
     1561 gaatacaagc tataaggaaa aaatggacaa agcaggtgtt gataaagatt atttaaaaca
     1621 agaaatatct agagatttag caatagataa aaataaaaag gcctttgaag atagaataaa
     1681 cataagtgat aatgatatgg aagcctacta tacaagtcat aaaaaagatt ttaatgtaga
     1741 agaagtaagt gcttctcaga tacttatatc gactttagat aaaaataaaa aagaagtaag
     1801 caaagataaa aaagaagctt tgaagaagaa ggcagataat atattgacta aaatcaaaaa
     1861 tggagaaagt tttgaaagtt tggctaaaaa atattcggat gataaagcaa ctggaaaaaa
     1921 tggaggacag ctaggatatt ttacaaagga tgataaaaat gctgagttta caaaggaagt
     1981 gttcaaatta aagaaaaatg aagtttcaaa tgtgtttgaa actagttatg gatatcatat
     2041 agtcaaagtt actgataaaa gagaaagaca aaaaagtttt aatgagtgtc agagtttaat
     2101 aagagaatct attttaaatg aaaagtatat tgagcatata aaaaaactga atgaagatgc
     2161 taaaatagac agatagaatt tctaacagct caatcctagc ctgtaatcgg agggagattt
     2221 tacaggaatg tttaagagtt taaagagaac gaaagttgag aaatacatta tcgataataa
     2281 agactctttt tatagaatag catatagtta tacgaagaat gaagaagatg ccttagatgt
     2341 ggttcaagaa gccatgtata aggcactata ctcagtggaa aatattaaag aagtaaatta
     2401 tataaagaca tggttttata aaattttagt tagaacgagt atagatttta ttagaaaaaa
     2461 ccgtaaatat aataatatga cagatattga ccttatagat gagactggag agtatgataa
     2521 atatactgat ttggatttaa gaagagcttt ggaagaatta cctattgaat acaaatctat
     2581 tataatatta agattttttg aagatttaaa gattgaagaa gtagcaataa tattggatga
     2641 aaatgtgaat actgttaaaa ctaggttata tactgcttta aaaaaactaa aattaaaaat
     2701 tgaagagtag ggataaaaag aatgacaagc aaagaaaggt taaataagtt aaaagatgag
     2761 tatcataaaa ttccaatacc caagaaatta gatactataa taaatcatga aaaaatagaa
     2821 aacatatatc gagagaaaaa taaaaaagta aatagattaa gatttaaagt tgcaatagcc
     2881 tttgcatgta tatttacagt attagtaaat ataagtcctg tatttgcaga caatttttct
     2941 aagatacctg taattggagc aatagtagaa gtaataacta taaaaaatta tagtttaaaa
     3001 agtgaaaatt atgaagctga gattgatatt cctaaaatac gaggtttaaa agataaaaac
     3061 ttagaacaaa gattaaacag tagttttatg gaagatggaa aaaggcttta tcatcaattc
     3121 caagagagaa tggagaagat acaatcatca aagaataaag gatataaatc tttaagtttg
     3181 agttatagtg taaaaaataa ttctaaaaaa tttctatcaa tagagatgac aaaaaatgag
     3241 atagaggctt catcatatgt gagtaaggta cattatacta ttgataaaaa aagacaaata
     3301 gttttgactt tgccaatgct ttttaaagat gataagtata taaaagttat aagtgataat
     3361 ataaaagaac aaatgagaga acaaatgaaa aaggatagta caaaatccta ttttatagat
     3421 caaaaaaaag acttgcctgt tgaagatttt aaaacaatta ataagtatca ggatttttat
     3481 tttaataaaa atgaagacct tgtaatttgt tttgatgaat atgaagtagc acctggttat
     3541 atgggcgcag ttgagtttgt aataccatat aaagtaataa aagatttgta aattgaatag
     3601 tagtttatat ataaatcttt taaggaggac aatatgacta catataaaaa acttaaaaaa
     3661 tctatattta aaattgctat attttcaata tgcttattag cttcattagt agatacaaac
     3721 cctatatttg caggtagttc ctttaatgaa acttcaaata gtgatacgat aaaggtgtta
     3781 aatgctaaga gtatcaactt taaacaagac aattatgaag tgaatgtgag tattcctaaa
     3841 ataaaaggtt taaaagataa aactttagag aataaattaa acaatgagtt tttgcaagat
     3901 gggaaaaaaa tttacagtga atttcaaaat aaaatgaaaa aggaagataa taagggtcat
     3961 agatacctaa atgtaatcca taatgtcaaa aataattcta aggacttttt atcaatagaa
     4021 atgacaaaag aagagataga agcttcctca tctataacta agaagcatta cactgttgat
     4081 aaaaatagag gaatactatt aaatttacca atatttttta aagatagtag atatattaga
     4141 gctataagtg ataatataaa agagcagatg agaaatcaaa ttaagaaaga tgatacaaaa
     4201 tcttatttta tagaccaaga taaagactcg ccagtgaaag attttgaatc tataaataag
     4261 tatcaagatt tttattttaa taaaaatgga aatcttgtga ttagttttga tgaatatgaa
     4321 gtagctcctg gttatatggg agcagtagaa tttgtgatac caaataaagt aataaaaaga
     4381 ctaagataga tatatagaag atttttagat agactggtaa ggagaattat atgaatatat
     4441 atgaacaagc aataagatta atagaaaata atgaggattt cgcatttgct acaataacgt
     4501 ctcattcagg ttcaacacca cgtgaaactg gagcaatgat gattgtgaaa aatgattcta
     4561 ctatatttgg ctcagtagga ggaggtagtg tagaagcaga atgtataaaa catgcaatta
     4621 atgttataaa aaacagagaa tcaatgttgt ataaatttac tctaaacaaa tctgatgttg
     4681 caaaactagg aatgatatgt ggaggtactg gagaaataca aatagatttt atagatagta
     4741 aattaaaaag taatatagaa aaatttaata agagactaaa ggaaaataca agtaaagcat
     4801 atatatttgg tgcaggtcat atttcaagag atgttgctgt gatactatct cttttagaat
     4861 ttagaacagt agtaatagat gatagagaag aatttgctaa tcatgaaagg tttccagaat
     4921 cagaagtagt agttttagat tcttttgaaa acataccaga ttttccaact gacgaaaata
     4981 gttatataat tattttaaca agaggtcatc tatatgattc aagtgcttta gaatgggctc
     5041 ttaaaagaga ggctggttat ataggtatga ttggaagtag aacaaaaatt ggattgactt
     5101 acgaaaaact catgaaaaaa ggttttaaga aggaagaatt atcaaaagta catgcaccaa
     5161 taggcataaa attgaatgct caaacaccag ctgaaatagc tgtatgtata gctgctgaac
     5221 ttataaattg tagagctaat aaggaaaaat aaactaatga acacagggag taaagatatt
     5281 gatactttaa aggttaattt tagagggatt gaaataaaag cagttttagt atataaaaat
     5341 agaaaaaata tttctataaa aatagatcca tttggcaaaa taactgtaat gagtcctcct
     5401 aaaatatcca aaaaaataat aaaagatata attattgaaa aaggagactg gatacttaaa
     5461 aagtcagaaa aatgtaaagg aagagaggaa gtatataaac aaagaatgtt tataactggc
     5521 gaaaaatttc tttatttagg tgaagagtac catttagtta taaaagaact gttgagagat
     5581 tctgtcaaaa aaagtaactg tagaataact attaacgaat atcaaatagt tgttcagaca
     5641 aatgatacaa gtacagattt tattaaaaaa tctttaaaat cttggtataa gatggaaagt
     5701 gaaagaatag tattagagag aatagatttt cttaaattaa attgtgaaat aatgaagcaa
     5761 ttagttccag cgagtgttaa ggtaaaagaa caaaataaga gatggggaag ttgtacagct
     5821 caaaaaaata tttatataaa ctcaaaaata tctatggcaa gattagatgt aattgattat
     5881 gtaataatac atgagtttag ccatttagta catatgaatc actctaaaaa attctatgat
     5941 ttagtaaaat ccattatgcc cgattataaa gataaagaaa attggctaaa aataaatgga
     6001 tataaaataa taatctgaaa acgattattc caaaatatag gcgtaaagta taaaagaagt
     6061 attattgtga aaaaaataaa taatctgaaa attttaaaaa aagtcattga cattgtaaat
     6121 gatagggaat ataatctata ttaacaacaa aacagaatta aaatataaaa ttaaaaaatt
     6181 aaaagcaatg acagagacaa caatactaat agtgttatcc acagagaacc aatgttgctg
     6241 agaattggtg ttaacagtag tataatgatc actctggagc ttttgtactg aatattttta
     6301 gtaagtacaa acgtatgtca gcgttacatg actaaggtta aggtaattaa ttacccgacc
     6361 tgaaaaaaga ggggtagatg tattttttat atctacaaat aaggtggtac cgcggaatat
     6421 aactttcgtc cttataagta atatttatat tgcttataaa gatgaaagtt tttttatttt
     6481 aagcgatata aataaattta atccatataa aattaataaa aaaataataa aaataaggag
     6541 ggatttttat ggaaaatact gtatatgcta aattaaacca aggaatagat tcttttttaa
     6601 gagtggctat gactctaaga agaagagagg ttgatataca atcaataagt atgactgtag
     6661 ataatatgaa taattcaggt ataaaattaa tagtaaatga agaaaagaca tccttagatt
     6721 ctgtcttaaa ccatatgaaa aaactacatg atataagaga gataactgtt gaaagaatga
     6781 gacattaatt aaatatattt tataaagcta aagataaata tgcttatttt aaaataaaaa
     6841 actaaagata aatattaaat aagaaaaatc ttagattaaa atataagtgt cattgaattt
     6901 attagtttat cacagttaat aaatttatta gtttgttgta gctaataaaa actgaaaatt
     6961 ctaaaattat aattgtaaga ttatataaat ttagaattta aatctgttga ataatattaa
     7021 aacaatgata atgagtatga atttctaata aaaataaata tcataaaaat aagaaaaagt
     7081 attgggagga aatgaaaatg gcaagaatgt attatgaaaa agatgtggat ttagaagttt
     7141 taaaaaataa aaaggttgca gttttaggtt atggaagtca aggacatgca catgcacaaa
     7201 atcttagaga taacggtgta catgtagtga taggtcttta tgacggaagt aaatcagcac
     7261 aaaaagcaaa agaagatgga tttgaagtta agagtgtagc agaagctaca aaagaaagtg
     7321 atttgacaat gatgttaatg cctgatgaaa aacaaaagaa agtttatgaa gagagtgtaa
     7381 aggataactt aaaagaaggc cagacattag cttttgctca tggattcaat atacattata
     7441 atcaagtaca accaccagaa tttgttgatg ttgtaatggt agctcctaaa ggacctggtc
     7501 acttagttag aaatgtattt acaaagggaa gtggagttcc tgcactattt gcagtatatc
     7561 aagactacac taaaaaagct acagagacag ttttagcata tgcaaaaggg ataggagcaa
     7621 caagagcagg tgttttagaa actactttta aagaagaaac agaaacagat ttatttggag
     7681 aacaatcagt actttgtggt ggaataagtg aacttataaa attgggatat aaaacgcttg
     7741 tagatgcagg ttatcaaaaa gaagtagcat attttgaatg tttacatgaa atgaaactaa
     7801 tagtcgacct tatatatgaa ggtggatttg aaagaatgag atatagtata agtgacacag
     7861 cagaatatgg agattatgta tctggtaaaa gggtaataac tgatgttgca aaacaaggta
     7921 tgcaaaatgt tttagaggat atacaaaatg gaaaatttgc aaaagcgtgg attaaagaaa
     7981 atgaagaagg acgtgaaaac ttcctaaaaa ctagagaaga agaatataat acagaaattg
     8041 ccgaagttgg tagaaatcta agaagtatga tgtcattctt aaaataaata tatattaaat
     8101 ggattagttt gagggaaaga gtggtgtata gtagtgcgta tgaatggagc aaaggtaatt
     8161 ttagaatgtt taaagaaaga gggaattgac acaatatttg gatatcctgg tggagccgtg
     8221 atacctcttt atgatgctct atacgattat tcagatgatt ttaaacatat aaggacatcc
     8281 catgaacaag gtttagttca tgctgcagat gggtacgcaa gaagtactaa tacagtagga
     8341 gtttgtttta caacatctgg tccaggagca acaaatgcca taactggaat agcaacagct
     8401 tttatggatt cttcacctat ggttgtaatt tcagggcagg tgccaactag tttattagga
     8461 aaagattctt ttcaagaaat tgacataact ggagcaacat tatctatgac aaaacataat
     8521 tatttagtta gaaacactaa agaattagtt cctacaataa aagaagcttt tagagttgca
     8581 aattcaggta gaaaaggacc tgtacttgta gatgttccaa aagatttatt tttagcagaa
     8641 atggatttta gtggtgagga ctatgattta tgtcaaatag atgattatat ggattataag
     8701 agtgattttg atctagatga tgaaactaat attaagcttt taaatgaagc aatagatatc
     8761 ataaaagaat ctaaaaagcc tataatatat gcaggtggag gtgttaaatc ttcagacagt
     8821 gaggaaattc ttgaaaaatt tgcaactaag atggatacac ccgtactaaa tacacttatg
     8881 gggcttggaa atatagatag aaagaatgaa ttatctcttg gaatggttgg aatgcatgga
     8941 agtagagaaa gtaatttagc actttctaat tcagatttag taatagcgat aggtgcgaga
     9001 tttagtgata gagtgataag taaaagttct gaatttgcta aaaatgcaaa aataatacac
     9061 atagacatag acccatctga aataagtaaa aatatagaat ctaatgtatc tttggttgga
     9121 gatgtaaagc tagtccttag tttattaatt gaaagggttg aaagtaagaa taatagcaat
     9181 tggaaagaag agataaagag atttagaaag agtgagggtg tacaaacata tgaatttcat
     9241 ccacaaaata tacttaaaaa aataaatgaa aaatatgaaa cattaaagaa acccactgtg
     9301 gtagtaactg atgtcggtca acatcaaatg tgggctgcta agtactggaa ttttaaaggt
     9361 aataaaagtt ttataacatc agcagggtta ggaactatgg gatttggttt aggtgcagct
     9421 attggaacta aagttggtaa tgtagataaa aatgtggttt tagtcactgg tgatggaagt
     9481 tttagaatga actgtaatga actagctaca gttgcaaatt ataatgtacc tatgcttatt
     9541 ttactactca ataatagaac attaggaatg gtaaggcaat ggcaaaagtt attttccaat
     9601 caaagatact ctcaaactga cattaatgaa aatgtggatt atgttaaact cgtaaatgca
     9661 tataacattg atggatataa ggtatccagt atggaagaat taggaaaggc gctagatatg
     9721 atagatttta ataaacctgt atttttgcaa tgtgatatag ataaggatta tgatgtttat
     9781 cctattgtag cacctaatga tgcattggaa aatttgatat gtaactaaaa cttatactga
     9841 atattgataa aattttttat taaattttta ttagtattca gtttttgttg tttagagata
     9901 gattggcatt ttttattttt gcaaataaat caatgactat ataattaaga acttattaaa
     9961 ttttaattat acaatttaga atagtattac aacttaagta aataataatt ttgaataata
    10021 atttacggag gtattcatat gtttaaacac gataaagcat tattaaaaga agtaaaagta
    10081 gaaagaccaa atcctcaata tgctgtttta atgcaagaac agttgggtgg tgcaaatgga
    10141 gagttaaaag cagctatgca atacttgtcc caaagtttta gaataaaaga ccctcaaata
    10201 aaagatttat ttcttgatat agcagcagaa gagcttagcc atatggaaat ggtagcacaa
    10261 actataaatt tattaaatgg acatgatgtt gattataatg cagttaatac aggcgaaata
    10321 gaaacacatg tattaactgg tctatcacca gtcttaataa actcatcagg agctccttgg
    10381 actgctaatt acgttacagt tacaggggat ttagtagctg acttactttc aaatatagct
    10441 tctgaacaaa gagctaaggt agtttatgag tacttgtata gacaaataga tgataaatat
    10501 gtaaaagaaa caatagattt cttacttaat agagaagaag cacataatgc tctatttaga
    10561 gatgctttaa ataaagttaa agatacaggt tcaaatagag attttggagt tacagaagat
    10621 tctaaattat atttcgattt gtctactcca gggccaaatc acgatactaa gatagatatt
    10681 aatcctcctt cttttgaaaa acctttaaaa aaataatttg atatttttta ttttgtttta
    10741 gtagttataa aaattattta ctagaagaat taattatatg aaaaattaaa tatgtatagt
    10801 taaaaatggg tatacactta ttataagtgt atatctattt gtttattgca aaacatataa
    10861 taaaataaca aatgaggtgg tattttggac agtaatagaa aacctaataa tctaataaat
    10921 gaaaaatctc catatctttt acagcatgct tataatccta taaactggta tagttggaat
    10981 gatgaggctt ttaaaaaagc aaaagaagag gataaaccaa tatttttaag tgtaggatat
    11041 tcaacatgtc attggtgcca tgttatggaa aaagaatctt ttgaagatga agaagttgct
    11101 gaaataatga atagaaactt tgtggcaata aaagtagata aagaggaaag acctgatgta
    11161 gatagtgtgt atatgacagt ttgccaagct atgacaggta gtggtggatg gccaatgacc
    11221 ataataatga caccagataa aaaacctttt tttgcgggaa catatttccc aaaatattca
    11281 aggtataata gacctggagt tatagattta ttagagaatg tatctgaaaa gtggaataca
    11341 agcagagata tattaataaa atctggagat gaaataatag aagctcttaa ggatgatttt
    11401 ggtgtaaaaa atactgaggg tgatttatca aaagagatgt tgagttcatc agttagagta
    11461 tttaaggcta tttatgatga aaagtatggt ggttttggta atgctcctaa atttccaagt
    11521 cctcagaatc ttatgttttt aatgaaatat tatagtatag aaaaagacaa agatgtgtta
    11581 aaaatggtgg aaaaaacttt agatggaatg tatagaggag gcttgtttga tcatattgga
    11641 tttgggtttt caagatactc tactgataaa aagtggttgg ctcctcattt tgaaaaaatg
    11701 ctatatgata atgctatgct tactatagct tttttagatg cgtataaaat aacaaagaaa
    11761 gagttgtata aagaaattgc tataaaaact atagattatg tagttagaga gatgaaggat
    11821 aaagaaggtg ggttttattc agcacaggat gctgatagtg aaggtgaaga aggtaaattt
    11881 tatacattta atcctcttga gataattgaa gttttgggtg aagaagatgg aattttcttt
    11941 aataattatt ttgatataac tagtagtgga aattttgaag gcaaaagtat tcctaatctt
    12001 ataaaaaata aagaatatga gagacataat gaaaagatag ctgaccttag taaaaaggta
    12061 tttgaatata gaaaagaaag aacatctttg cataaagatg acaagatact tacatcatgg
    12121 aatgcattga tgatagttgc tttaacaaag gcatatagta ctttaaaaaa tgacatatat
    12181 ttagagtatt caaataagtg tcttaacttt ataaataata atcttgtaaa tgagtcaggt
    12241 agattattag ctagatatag agatggaagt tccgactatt tagcatattt agatgattat
    12301 gcttttttaa tctgggcgta tatagagctt tatgagtcaa catttaatat gaaatattta
    12361 gaaaaggctt taaatttgaa tgaaagctgt atcaaccttt tttgggatta tgaaaaaagt
    12421 ggattttata tatatggtaa agatagtgaa aatttaatag caaggccaaa agatttgtat
    12481 gatggagcta ttccatcagg aaattctgtt caattataca atcttataag acttgcgaaa
    12541 attactggag ataatagact tgaagaaatg tcatataaac aattaaaact gtatgtggat
    12601 aatgtaaaga gttctccagc tggctacagc ttttatatgc tttcattgat gtttgaactt
    12661 tattctacta aggaaattat atgtattttt aaggaagatt ctgatttaat tgcctttaaa
    12721 gaattgataa gtgaaaattt tataccaaat gcaacttttt tagcaaagaa atacaatgaa
    12781 gaaaacacta ttataggttt tttaaataac tatagattga aggatgataa gattagttat
    12841 tacgtgtgtc aaagtaatag ttgtagtcaa ccaattaatg atttacaaaa attaaaagat
    12901 atgattttag gagtagaata atcataatag tgaaaaagga gatgctttaa aataaaagtc
    12961 taatcatctc ctttttttgt acaaaaaaca gaaaaagagt tcctgagaac acctaatcta
    13021 tgtaatgatt gttttgttaa aaaataaaaa tgacattata gttatataaa atattgtact
    13081 ataaaaaagt agtattatag atgcgatagt attaatcgtt tttatttact ctctttcttc
    13141 tttttgcaaa ttcttcaagt gcttcagatt taattttatc tatttcttca tattccataa
    13201 aattagcatc ttcttcaaag ccatcttgtt cttcaccatt aaaacgatac atttttttta
    13261 gtaaactcca taaggtctct aattcatctt tggtaaattc gtgaaataca tctgccataa
    13321 agtttattcc agtcctacta catgttacca taaccttttt tcctaagtct gtaactttaa
    13381 cattaattgc tctcttatca tgaggacttg gaattacatc aacatatcca tttttttcaa
    13441 gattagcaac tagtctattt atattttgtt tacttgtacc catttttctt gcaatattat
    13501 ttaatgttgt ttcttcctct ggtaaatgaa gaattgataa aatagtcata tactgtcttg
    13561 aagtaagtat accaaaatat ttatctcctt ctatttgtat tttgtttgta agggtaaaaa
    13621 gagttgcata tgtttgattc attaaaaaga gttcttttag ttcatttgaa taatccatga
    13681 aatatatctc ctttattttt agatgttgtt ttgtcgaaaa cagaatatca tgaattaatt
    13741 ctaatgtcaa tatggtgtca aaatatatta aagtattact tttttatgta actattaatt
    13801 aattaaatta ttgtataatt ttatgtaaaa acttctatgt accaatgata acttctatga
    13861 ggaattaata attcttaaat tttttagaaa gattttgact tgaaaatact tgtaatataa
    13921 tatataaatt aatatactga ataaaatcaa tctggaggga taattatgaa gtgtacaaac
    13981 tttactttca aaggagagga aggtttagat atatatacat ataaatggga agatgaaaat
    14041 ataaaaaaac caaaggcagt tatccaaatt gcacatggaa tggctgaaac tgcacaaagg
    14101 tatgagactt ttgccaaggt acttactaaa aatggttata tagtatatat caatgaccat
    14161 agaggtcatg ggaaaactgc aaagataata gaaaatgttg gacatttagc tgaaaaagaa
    14221 ggatttagat gtcttgtaga ggacatgtat actttaacaa atattataaa gaaagaaaat
    14281 gaagatctgc caatttatct atttggacac agcatgggtt catttgcaag tcaaagatat
    14341 ataatggatt acagtaataa tttatctggt cttatactat gtggttcaaa tggaaagcaa
    14401 ggaatcattc taaatcttgc tcatctgata atcaatcgtg agattaaaaa gtatggaaga
    14461 cgttctaaaa gtaataaaat aaataatttg atatttggcg gcgaaataat aagaagaaat
    14521 gaaaagacta agtttgactg gttaagtaga gataaagaac aagttgaaaa atatataaat
    14581 gacccatttt gtggagtagt gtgtagttgt ggcttttttt atgatttagt tcaagggtta
    14641 aaagaaattg aggataaaga aaacttaaaa aaagtgccac ttgatatacc tatttacata
    14701 atatctggag ataaagaccc cataggaaaa aatggtaaag gagtcttaag actaagggat
    14761 agatatataa aattaggagt aaaagatgta acttgcaaac tttacaaaga tggaaggcat
    14821 gaattgttga atgaaataaa tagagaagaa gtttttgagg atataatttg ttggttaaat
    14881 aataaaatag aaatattgta ataaaaaaat ataatgatat atttacttta aatttattta
    14941 tatataatat ttgaaaagtg gattatgtat atgcaaagta gaaaagagag agcccagaag
    15001 tgattttttg ctataatgcc atttattgaa tag
//
LOCUS       DAYAOY010000103        14921 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYAOY010000103 DAYAOY010000000
VERSION     DAYAOY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.103"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            227..1159
                     /locus_tag="ACGI6B_003224"
     CDS             227..1159
                     /locus_tag="ACGI6B_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFG9752640.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1134..1961
                     /gene="panB"
                     /locus_tag="ACGI6B_003225"
     CDS             1134..1961
                     /gene="panB"
                     /locus_tag="ACGI6B_003225"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFG9752641.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1980..2828
                     /gene="panC"
                     /locus_tag="ACGI6B_003226"
     CDS             1980..2828
                     /gene="panC"
                     /locus_tag="ACGI6B_003226"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFG9752642.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3239..3421
                     /locus_tag="ACGI6B_003227"
     CDS             3239..3421
                     /locus_tag="ACGI6B_003227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752643.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4248..5162
                     /locus_tag="ACGI6B_003228"
     CDS             4248..5162
                     /locus_tag="ACGI6B_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HFG9752644.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5552..6199
                     /locus_tag="ACGI6B_003229"
     CDS             5552..6199
                     /locus_tag="ACGI6B_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFG9752645.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(6366..6935)
                     /locus_tag="ACGI6B_003230"
     CDS             complement(6366..6935)
                     /locus_tag="ACGI6B_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752646.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(6940..7839)
                     /locus_tag="ACGI6B_003231"
     CDS             complement(6940..7839)
                     /locus_tag="ACGI6B_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9752647.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(7850..7999)
                     /locus_tag="ACGI6B_003232"
     CDS             complement(7850..7999)
                     /locus_tag="ACGI6B_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HFG9752648.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(7992..8576)
                     /locus_tag="ACGI6B_003233"
     CDS             complement(7992..8576)
                     /locus_tag="ACGI6B_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFG9752649.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            8965..10290
                     /gene="cdeA"
                     /locus_tag="ACGI6B_003234"
     CDS             8965..10290
                     /gene="cdeA"
                     /locus_tag="ACGI6B_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HFG9752650.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(10385..11179)
                     /locus_tag="ACGI6B_003235"
     CDS             complement(10385..11179)
                     /locus_tag="ACGI6B_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752651.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(11180..12055)
                     /locus_tag="ACGI6B_003236"
     CDS             complement(11180..12055)
                     /locus_tag="ACGI6B_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9752652.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(12059..12427)
                     /locus_tag="ACGI6B_003237"
     CDS             complement(12059..12427)
                     /locus_tag="ACGI6B_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9752653.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(12610..13269)
                     /gene="deoC"
                     /locus_tag="ACGI6B_003238"
     CDS             complement(12610..13269)
                     /gene="deoC"
                     /locus_tag="ACGI6B_003238"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFG9752654.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(13305..14504)
                     /gene="murJ"
                     /locus_tag="ACGI6B_003239"
     CDS             complement(13305..14504)
                     /gene="murJ"
                     /locus_tag="ACGI6B_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFG9752655.1"
                     /translation="MQNSMKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVG
                     FGSIPYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFK
                     DDSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFT
                     ASIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFE
                     RGAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIV
                     LDLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLI
                     MGVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAK
                     RKLHLKK"
     gene            complement(14569..>14921)
                     /locus_tag="ACGI6B_003240"
     CDS             complement(14569..>14921)
                     /locus_tag="ACGI6B_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9752656.1"
                     /translation="TKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEY
                     KANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASIN
                     ILKEGLRLITIQNK"
BASE COUNT         5364 a         2180 c         1811 g         5566 t
ORIGIN      
        1 atattttata aatatcataa tttttttact gatattttat tatataaatt ttatagaatt
       61 aattgtcaat tttttatgaa aatggtaaga tatttatgga tggtatcttg ttataagaac
      121 tatactttaa atgtttttaa ttattttgtc ttatgtaact catatcctca ggaatgagat
      181 tagaaattaa gatgttgtat cacaaagtgg aggtgatact attaaaatag gctttattgg
      241 ttctggaaaa gttggaacat ctttggcaaa ttatttttta tcaaaaggtt attgtgtttc
      301 aggcttttat ggaaggaatc aattatcttt attagaatca acaaatttaa caaaaacaaa
      361 gatttacagc aatttgaaag atataattta tgaaaacgat atattattta ttacaacatc
      421 agatgattct attgaaatta tagacaaaaa actttccaaa tttaatttaa aacataaata
      481 catttgccac acaagtggct ctttaaaatc tagtatattg ttctgttcaa aaaaagcagg
      541 tgcattaatt tattctatcc atcctatatt tgcattttcc aataaaaata ctgacataaa
      601 aaaaatgaaa gacatttgtt tttccataga aggtgataat gaacaagatg atttagttat
      661 tcaaaatttc atagatgatt tgggaaacca attttttata agagataagg atacttcatc
      721 cacttatcat ttagctaatg ttttagtttc aaatttagta ttatctttac tagatatagg
      781 agtaagttac tttattaaat taggtttatc tgaagaggaa tctttaaaag ctatcagtcc
      841 tttagtaaaa aaaaacatag aaaatatact tgataatggc tttactaaag ctcttactgg
      901 tcctgtttct cgtggagata taacaccaat aaggaaacat ctatctgtat tggataaaaa
      961 agatgaggat atttacaaaa tactatcctt aaacctacta aaaataattg ccttaggaaa
     1021 tgacaattca ttaaaaggta taaaaaatat tactatagaa aatgcaatcg aaaatttaat
     1081 aagtaaatct gaaaaataca aagaaattta caaactttta ggaggaaaaa acgatgaaaa
     1141 ataccataca gacttttaag aatgctaaat atgaaggaaa aaaactatct atgcttactg
     1201 cttatgacta ctcaattgca aaaataatgg atgagtgtga tataaatggt attttaatag
     1261 gagattcatt aggaatggtc ataaaaggtg aagaaaacac tctatctgtt accatagacg
     1321 aaattatata tcatacaaaa gctgtaaaaa atggcgtaaa aaatgcctta atcgtaagtg
     1381 atatgccttt tctatcttac catgtttcta tagaagatgc agttaaaaac gctggtagac
     1441 ttataaaaga aggaggagct catgctgtaa agttagaagg tggctcaaac gttataaagc
     1501 aaatagaaag cattgtaaat gctcaaatac ctgtaatggg gcatttagga ttaactcctc
     1561 aatcagtaaa ttcatttggt gggtttaagg ttcaaggaaa taccagtgaa acagcaagac
     1621 aacttattga agatgctaaa ttaatagaaa aagcaggtgc attctctatt gtccttgaag
     1681 gagtgcctac gaaaatagct gagatggtaa ctaattctat ttcaattcct acaataggta
     1741 taggggcagg aattaactgt gatggacaaa ttttagttta tcaagatatg ttgggaatgt
     1801 ttggagattt tgttcctaag tttgtaaagc agtatgcaaa tataggagat ataataaaag
     1861 attcaattaa aaattacatc ctagaagtta atacaggtgc atttccacaa gaaaaacaca
     1921 gcttttcaat aaatgaatct gagttagaaa aattatacta atattaagga tggtacaaaa
     1981 tgcttgtaaa agaaattaaa ttactaagaa atataatcaa agattggaga aaacatggct
     2041 attctatagg ccttgttaca acaatgggat ttttacatga aggacaccaa agtcttataa
     2101 aaaaagctgt aaaagaaaat gataaagtag tagtgagtat atttgtaaat ccaactcaat
     2161 ttggaccaaa tgaagatttt aatagctatc caagagatat agataaagat tttaaatatt
     2221 gtatggatag tggagcaaca gtagtcttta acccatcacc agaagaaatg tatttaaaag
     2281 gtaattgtac taccattaac gtttctggac ttacagattt tctttgtgga gctaaaagac
     2341 ctgttcattt tggaggtgtc tgcctagttg tttcaaagtt tctaaatata gtaacacctg
     2401 ataaagctta ttttggagag aaggatgccc aacaattagc tgtaattaaa agaatggtca
     2461 aggatttaaa catagataca gaaataatag gctgtcctat aattcgtgaa aatgatggat
     2521 tagcaaaaag ttcgagaaat acatatctct ctgaggaaga acgaaaatct gctctaatat
     2581 taaataagag cttaagttta gcaaaagaag aattagtaaa aggcaatcta aatccagaga
     2641 atataaaaga attaataaca gctaaaataa attctgaaca cttagcaaaa atagactatg
     2701 ttgaaattgt agatagtgag acattacaac ctgttaagca aatagaacat tctatacttg
     2761 tggcaattgc tgtgtttata ggaaaaacca gactaattga taattttaca tttgagttaa
     2821 atatatagtc tataatttta tataatatat ctaaaaaata atagaatacc taaaaagtaa
     2881 taaattacct aaaaaataat aaattgctta gaaaataata gattacaata actaaaattt
     2941 taaaattaaa ttccttaaaa gtatgattaa ttataattat ataagtatat tcttattaca
     3001 cttatattaa tttatattaa atatctacta actcaaatat tcataattaa actttaaact
     3061 ggataaattt tcttttaaga atgaaagttt atccagtttt gcgattataa taaccagttt
     3121 tacatatcca cttgtaaaat atatctaagc aatatatcat actatataga tttatgagtg
     3181 aaataatatc tgctatttac tgaatatttt tatttttcgt tttttagggg gattagttgt
     3241 gtttaaagat aagattgatg aatgtgttca tataatgact gcatatattg ctagtttaaa
     3301 agaatactat tcattcatag aaactcaaat tggtgatttt attaaaaaat atggagaaga
     3361 tgttgtagag ttatgtctcc atagagtgat gattctacta tgcgaatgtg gtttggcata
     3421 aaattaaatt tattaaaatg ggtaagaaga atcctatata atattcttct tacccatttt
     3481 aatatatcaa tttcatagct tatcgtttaa aatattttac atccattact agacaacttt
     3541 acattctctt tttaattcta gtatatttaa atcattttag ttgctcctta tttattctta
     3601 ttacttgttt tatttataaa ataattcttt ttaataatgt tttttgttga tattcctatc
     3661 acacatccaa ttatttatca ttgtcttgaa attgtttgca tacttaattt aatccaattg
     3721 aaacacccca gcatagaatc ccctgtaaaa tagaagttgg attaaatcct aacatctata
     3781 cagaaaatac tactccaagc acaagcaata taatggaata tatttatcac ttaatttttt
     3841 atacttttta aatcccatac ctaaaacatt aagtgcagct attagaatta ataattgttg
     3901 tggtataaaa ttatgcttgc ttcatattta ttcatttttt gctataaaag ttcttgtttt
     3961 acaaaaaaca tttgttttgt atcaaaataa tacttataaa ttacataatt ctcataaaaa
     4021 tttctagtat tacttgtact cttaataaat aatagccaac aagtacctta aatcattata
     4081 tctatatcta ataagaaaca cagtaagata atcttttcgc ccataactta ataaaaactg
     4141 ttgatattag tagcaattga atttagttaa tttatttata atatacataa atttaaatat
     4201 tacgaataaa gtattataaa taggatactt tctaagggga ggaaattatg atagataatc
     4261 aaaaatatgt tattttatca ctagaattac atttattttt ttcaagaatt atgaaagagc
     4321 atgctctttt tttagaagca ggattcacaa ataaaaatta taatcttgct atggaagctg
     4381 accactataa aaagcaattt gaagatttat tatcatacac tgttagtgct agtaatggta
     4441 taattagacc tgatatatta tattcagaag agcttgtaac tactctcaca tcagttgcag
     4501 aacaaaaaac agaagagttt acagggatag aaataaacaa aaacatcact acaagagaat
     4561 taaatctaca aagtggtgta aacccacaag ttggtcaaga cttagtgaac tatgtagctc
     4621 aacttaactc tgatgcaata agattacttg atgggcttat taattttaaa gaaagagtct
     4681 tagatggtgt actatcatgt actatattta catcaaacta ccctctactt cttgaacata
     4741 taatacatga agcaaattta tatcgttctt atgtagttga ccttgaaaat aaaatagata
     4801 ttgagtcaaa aaacgctaaa gaaatagaat tattctggga ccatattatg atggaacatg
     4861 ctctgtttat gagaggatta ctagacccct cagaaggtga actaataaat acttcaaatg
     4921 attttgctat aaaatttaat gaattaattg aaaaaacaaa cgaaatgact gattctaata
     4981 tcaagaacat tacagaagaa actctaaatg aaactgttga gtttaaagat tttaaagaag
     5041 caggagcatc aggaatagaa cagtgtaaga taaaatctat aatattacca cttttagcag
     5101 accatgtttt aagagaggca aatcattata ttagaatatt ggagagttat aaaaacatgt
     5161 aattaaaaat tacttttaaa ataatctttg catatttaga taaaataaag gtacagttaa
     5221 ctgtaccttt attttttata aacttataaa atttaaacaa gttattcata aaataaaata
     5281 ataatcatct ataacccaca actttatttt atattaaata aaaagtatta gtggttatgt
     5341 tttataagta tttctttatt cagttattta ttaaaaacta atagtaaatc ttatactaat
     5401 cgcatattta tgttataact atattgtaac tattttcgta atcttaggaa aagattactt
     5461 ataaaattaa ccttatacaa taatatatcg tataatctta attagcataa tagttacaat
     5521 attaaaattt aaatatggga gtgattttag tatgataaga caaacaccat tagagaaacg
     5581 atatgacaag ctaggtcctc atattgtaac agcactcaaa aaacgttatt ttgatgctta
     5641 ttactgtaaa actagaaatg aagctttgca acaaatttta gatttaattc ctcaaaatca
     5701 tgtagtatca tggggaggat ctgaaacact taaagaaatg ggcgtacaaa ctgctgttag
     5761 agagaaagga tttaaagtta ttgatagaga tttagcaaaa actccagaag aaagaactga
     5821 aattatgcgt caagcacttc tatgcgatac ttttttgatg agtagcaatg caattagtga
     5881 agatggacaa ctctttaata ttgatggaaa tggcaatcgt gtagctgcaa tgatatttgg
     5941 tccaaaaaat gttatagttg tagctggtat gaataaaatt gtaaaaaaca ttgatgatgc
     6001 aatacaaaga actagaacaa ttgcatctcc agcagttgtt cagcgttttc aagatgtcaa
     6061 tacaccatgc tatattaatg gaagttgtat agattgcaca agtcctgaaa gcatttgtgc
     6121 atacatggtt actacaagaa tcagtcgtcc agcaaaaaaa ataaaggtaa tattggtagg
     6181 agaaaatctt ggtctataaa atatacatac atgtaaatag ctctataaat aataaaatca
     6241 tatgaaataa cttatttcta aaagtggtag aataaaagct tctacaagac tttcattctg
     6301 ccacttttat attcttttaa tttgtgtttc tatattatta attgctccaa ttttcatata
     6361 tattactaac tatttttatt ttcataattt ttttgttctt catcaagctg tttataataa
     6421 tctttattat agtcagctag ataatctata ccacctcctg taatcacatt taataatttc
     6481 attgcagatt cttgattaac tttacaaaca taattatcac cagatttaac aagctctaat
     6541 gtaatattaa attctttttt ctttgcagaa tcaatatctt tcatagttgg atttggcatc
     6601 atttctccta gtttaggagg attctttttt aaaatagaaa ccatatcagt attgttaatt
     6661 tttaaatcta cagtcacttt atccttatta ttttttatat ctgaaatttc ccaagtcata
     6721 tttttagaat acattttaaa tccttcttta tcttcgtata taagattacc taaccctgat
     6781 tttgggaaat acttatcaac agtatctgca tcttgtgttt ttattgaatt aaacatacta
     6841 tccacttcat tttttatctt attatcatta gtacaaccag ctaacaccag tactgatatt
     6901 gcaaacatta taatacatag aataaatttc ttcattaaat tatatccccc tatacttttt
     6961 tgcacaaata ttattctctt ttttatcttt tgaaaaagtt gtagtaattg cattttgtgg
     7021 acaaactttt atacattcac cacacctaat acagtctgga ctatttacat ctttatatat
     7081 tggtatatca agcttacatt ttgtttgaca tgctgtacaa tttgtacatt tatctttatc
     7141 aattttaaat ctatatattg aaataggatt aaaaaaacca tagattgctc ctaaagggca
     7201 tatatacttg caaaatggcc tataagtaat gattgacatc aataatatca ataacaaaat
     7261 acttaacttc cagctaaata aaaatcctat tgattctctt aaattctcat ttcctaaaat
     7321 aagtggtatt cctcccaaaa gtgttcctga agggcaaatc catttacaaa accaaggatt
     7381 tccaccccct gctacattta caatagcaac tggcaaaata attacaaata ttattaagac
     7441 tatatacttt agatatttta gatatttatc accataaact ttttttatct ttactggaaa
     7501 gggtacttta tgtagtaaat cttgtacaag tccaaaggga caaagccaac cacaaacaaa
     7561 tctgccaaaa aaagcaccaa atgccattaa aaaaccaaca acataaaatg agaatttata
     7621 ctctctgctt gataaaactg cttgtaatga acctattgga catgctccaa gtgcacctgg
     7681 acatgaataa caattcaatc caggtacaca taatttttta ctttctcctc catatatagt
     7741 tccttctaaa aatccattta catatccatt tgttaatgct gtaaatgcaa cttgagtaat
     7801 aagtcttata ttattattta ctttagataa taacttcatt cctcttctcc taaccaatac
     7861 caatacactc aagacaaatc ataattgctt tagttaatac tgtatttgtt tctccacgat
     7921 atgtacctaa tgcaataaat ataatactaa ttgcaataat agaaatgcct tttttattat
     7981 ctttaataaa gttactcatt ggcttttacc atttttagtc tttcatctat aatttttttc
     8041 caatcttcct tactatttgc acctacaacc ttctcaccca caaagtttcc tttcttgtct
     8101 acaaaataag ttgttggaaa ctccataact cttttaaaat cagttttttt aagttcttca
     8161 gatacagtca attgttggta agttgcatct gactttttca taatgtcttt aactaaattt
     8221 ttctcttcat ttgaaagacc agttctcata tcagttttat caacttctac aactaatccc
     8281 tttatagcta cattggaatt attactctca tattcttttg ataatgcttc aagctctggc
     8341 atttcaccca cacatggtcc acaccaagta gtccagacat taataagagt aagttcttta
     8401 tttgaaaata tctcttttgt taccttattt ccatcaacat cctccgtatc taaattacta
     8461 aaccattcac tattattctt gtttttttct actggcttag attctttttt gctacatcca
     8521 gaaatcatac ctaaaacaca aatacatcct aaaactacta tgaaaaacct tttcatatct
     8581 cacactccta gaatttatat agtatataaa atcgctgctt gaaataaaaa taacttatct
     8641 tctatattaa agtaattata tttaataaat aagagctaat ttaaaaaaag atatacagtt
     8701 aaaatctatt tcttttcaga caaaatctta tatatatata atagcttgta aatcttaaga
     8761 ttatatacat atatttctaa agattttctt aagaaacagt tagattttac taatataagt
     8821 ttattaatta gcattatact aatttatctt aatatgattt tttgttgatt ttaagctgtt
     8881 tatcgtataa ataaaatttt tcttgactat atagttacta tatagcttat tatatataga
     8941 gagtaataaa aggaggtaaa atttatggaa aatttattta caagaaaatt cactactttt
     9001 gaatttctaa aatttgtttc tcctgcaatt atatccatga tatttatatc tttgtacaca
     9061 ataatagatg gcatctttgt atcgacatta gttggttctg atgctcttgc tagtataaat
     9121 attgtactac ctataattaa ccttgtttgt ggatttggaa taatgatggc aactggtgga
     9181 ggtgctatcg tttctatacg tatgggtgaa aatagacagg atgaagccaa ctctacattt
     9241 tcttttatag ttttgttttc attgattgtt gggattttat tcacagtaat ctcatatttc
     9301 tttatcaaag aaatatctat tttgcttggt gcaacagata agttattacc atattgtata
     9361 acttatggta aagttatgat tttatgtaca ccattttata ttttaaaatt tatatttgag
     9421 tactttgcaa gaactgatgg aaattctaaa tttagtttat ttctatcagt cattggtggt
     9481 gtaacaaata taatattgga ttatgtattt attaaatatt ttggaatggg tcttttagga
     9541 gctgcagttg caactgctat aggtattatt ttaacttgtg ttttaggtat tatttacttc
     9601 ttatctaata aatctacact aaaattaaga aaaccaaaaa ccgattttag acttataaga
     9661 gatactatga taaatggttc ttctgaaatg gttacagaat tatctacagg aattacaaca
     9721 ttcttattta atgtagtagc tttaaaatta gcaggagaaa atggacttgc tgctcttact
     9781 atagtattgt atgctcattt tttaatgaca tcagtctatc taggattcgc tgctggagtg
     9841 tctccattaa taagctataa ttttggtgct gaaaacagtg ataaattaaa agaaacattt
     9901 aaacattctc taaaatttat atttgtttct tctcttttag tgtttatttt tgctttagta
     9961 tttgcaccat ttattgttag ggtctttgta agtccagata acacagtatt taaactagcc
    10021 ttacaaggat taaaaatatt tgcatttgct tttttgtttg ttggtataaa tatatttgca
    10081 tcaggatttt ttacagcatt tcacaatgga aaaatttcag ctattatatc ttttagtcgt
    10141 gcctttgttt ttataatcat aggaatcata attcttcctc ctatgttaaa catgactgga
    10201 ttatggctta cagttccatt tgctgaggtt ataaccatat ttatatctat tctatttata
    10261 aaaaaatata aatgtagata taagtattaa aaattaatac ttaaaatcaa catttattaa
    10321 aactaataaa cctgctttaa aatacaaatt tacaaatttg atttttaagc aggttttatt
    10381 ttagttataa taagtcattt gcatactctt taattggtgc ttttctaagt aataaaatta
    10441 taaatattat gcaaataact ataattccta cttttgttcc aaataaaata aaaggattct
    10501 gaaatgaata aataattttt tctaaataca atagcatttt atgcatattt ttagttgcaa
    10561 aattaactat ataaattatc attaatatcg gtaatcctat caatatagta atactagtaa
    10621 cttttctaac tctatatgta aaagctccaa gtaacactcc tataaaacct atacatattt
    10681 catttataca tatttgtaca aaataattta aataagttag tggcatactt gatgttgaag
    10741 gatctatcag tgctgatata gatgtccaaa tcatatctct ctttagtaaa actgatagtt
    10801 ctatactgta acctgttata atttctataa ataacttcga taaaaaaatc aaaactatac
    10861 ctaaaataga aatcacaaag cttaaaataa ttaaatctat gacaatagct tttatacaac
    10921 tttttctatc tgctcttata cttaaagctc ctgaaaagtc tttatttgct atatttactc
    10981 catacacaaa tagcagtata gttatatatg ttaaaatagg attcacaatc gtggataaat
    11041 tagttttaga tgcttctcca aaaatcttga gaataaacac tggcaaaata ttcataacaa
    11101 ttcccacaat cataaatata attatataca atttagtatt tttattaaaa aattttatat
    11161 atggctttaa ttcattcaat tacaattcct ccttcttagt catgttaaca aacatatctt
    11221 gtagccctat gtatcctaca tcaatatttg aatttttaag tattttttca tcactttcac
    11281 taaaatcacc atagtaaaaa taagcaactg catttccaaa tgccttttta ggttttatat
    11341 tttttataca ttctaatttt tctaactctt ctttatttcc agtaatataa tgagcttttt
    11401 gttttatgac atcaatttct tcatctataa taatttttcc ctcatttaat attattacat
    11461 gctccagtaa atcatctatt tcatcaatca aatgagttga tattattatt gtccttggat
    11521 tttttatata actatctaaa attatgttat aaaactcttg tctatttact gcatccagac
    11581 caattgttgg ctcatcaaat atggtgatag ctgaattaga acaaatacct attatatttg
    11641 aaagtatagt tttcatacct cttgacagtt gtttatactt ttttttagta tttaaatcaa
    11701 aatatttgca taattcttct tctaagcctt tatcataact tttataaaaa ctagaagaaa
    11761 attcaaatat ctgacctact ttaaaatctg gactataaaa ttctttttcc ctaacgatac
    11821 aaacttcttc caaaactttt atatcttctt ttgggttctt tcctaatatt tctatctcac
    11881 cttcattttg aattaactga tttaccatta tatttaataa tgttgttttt ccagctccat
    11941 tcttacctaa taaaccatat atcttatttt tttcaaagct tagagacata ttattaaaaa
    12001 tattttgctt cttaaaactg tgagataaat tttctatctt aatagaacta ctcatatatt
    12061 aatcctcctt gaaattaatt ataatatcaa ccaattcttc tctatttatt tctaatttgt
    12121 tagcttcttg aagtaggttt tttacaaaat tatgtttaaa attttctttt ctagcttctt
    12181 ttataatagt ttttgcacca tctgatacaa acatccctat ccccctcttc ttatataaaa
    12241 tatttttatc aaccaatata tttatacctt ttaatactgt agctggatta attttataaa
    12301 gctttgaaag ctctgttgtt gatggaacct gttcttcttc ttttattcca tttgatagta
    12361 tctcatcttc aattgctcgt gctatctgaa taaatatagg ttcttcatta tttaatacta
    12421 atttcatagg ttcctccttt ctccatcagt tagttactca actaattaac tatataactt
    12481 ataatatctt gaaaaatatt ttctgtcaat actaaataaa aaacagaggg aaaattccct
    12541 ctgttttttg ttctttatat ttataattaa attttgattt ttgtaatcct atcccataag
    12601 ttaaaaactt taataatctg ttgtatcttc attttttaat attttacata ttgagctagt
    12661 tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg atttaactcc
    12721 ccctgcagct tttattttta cattttttcc aacatgctct ttcataagtt ttatatcttc
    12781 taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg aacctgacat
    12841 tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg tctctataat
    12901 gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt ttcctatatt
    12961 atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt catctgctcc
    13021 attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat atcccaaagg
    13081 gaaccctatc actgtacata tctttatttt accttttaga tattctgatg ctcttttcac
    13141 atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat cacataaaat
    13201 tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg tttttaatat
    13261 atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt ttttaaatgc
    13321 aattttctct ttgcaatgtt tagtatcata tcaacctctt caactttaaa aataatcatt
    13381 agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac aaactcatta
    13441 aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga taaaacaccc
    13501 ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc ttgtccaaaa
    13561 tatccaactt ttctctttag gttcaagaag agtaacaata tacatgcaat tgatgatgaa
    13621 ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa gtccaatact
    13681 atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc ttgtaatgaa
    13741 taaaaaacct ttcccaaaat atctcttaag ccaaatgcag tcataccaac tgcataaaaa
    13801 attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct ctcaaaaatg
    13861 attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat catagaaatt
    13921 attatcatat ttatactgga tttaactgaa ctagtaaact tcttttgatt attttctgca
    13981 cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa tgtccctgta
    14041 acaaaaccat ttaatctatc tgcataagta agtgctggaa tgcttccttt aactaatgtt
    14101 gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc tataaatact
    14161 ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa gtagtataag
    14221 tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac taccatagca
    14281 accacagcac caattggaag tatgtatggt ccaaatatgg tacttaacat tattgaaata
    14341 attattacta tattataggg tatacttcca aatccaacta caataaagtt ctctttaatt
    14401 tgtaaaaatg ctgacataac actcgtaata cctatgaata ttattcctgt tctgaacctc
    14461 ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcatactaaa atatgtctta
    14521 ctaattctcc acaggctatc cccgtagttc ctacggttct tacatacgtt atttattttg
    14581 aattgttatt agtcttaatc cttcttttaa tatatttata cttgcattta tatctctatc
    14641 gtgagtttca ttacaactag gacaaatcca ttctcttata tttaagttct taacttcctc
    14701 gtttttatac ccacatttat tgcatatttg tgaacttgca aagaatttac ctacctttac
    14761 aatttgtcta ccataccaat tagccttgta ttctaattga cgaataaact ctgaccatga
    14821 tacatcagaa attaagcgag atagtttacg atttctaatc atattcttta cttgtaaatc
    14881 ttctatgcaa ataatatcgt tctcttttat taatttagta g
//
LOCUS       DAYAOY010000104        14912 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYAOY010000104 DAYAOY010000000
VERSION     DAYAOY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.104"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..915
                     /locus_tag="ACGI6B_003241"
     CDS             <1..915
                     /locus_tag="ACGI6B_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752657.1"
                     /translation="KITLLQALPFLTVSSYNSITATFKADHTDPTLLTKIEYFIDDVL
                     KETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPEDVNLQ
                     DISSKLIEVNAGFKTGKTSIINTLALKNIEANLNNTLVELSEKIKTGFDSSDATAEQL
                     QNQITNLNNQLSQRKRWAKGTYTFTQSDYENFYSRYSSAENYAKSLTVPINLNFIPSL
                     VIVEKLCFYGNGYTVNFYAINNLNQLASVGRVPKSSGEYRFCAFMFVKTITSKEFSLY
                     TYRDEYTDSSSVVYRCKVGESFTWYAYE"
     gene            936..1229
                     /locus_tag="ACGI6B_003242"
     CDS             936..1229
                     /locus_tag="ACGI6B_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752658.1"
                     /translation="MERGNRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQILLNENEKVGGIL"
     gene            1229..1411
                     /locus_tag="ACGI6B_003243"
     CDS             1229..1411
                     /locus_tag="ACGI6B_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752659.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSEVVEGAELTK"
     gene            1452..1682
                     /locus_tag="ACGI6B_003244"
     CDS             1452..1682
                     /locus_tag="ACGI6B_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFG9752660.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            1702..1959
                     /locus_tag="ACGI6B_003245"
     CDS             1702..1959
                     /locus_tag="ACGI6B_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9752661.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            1959..2765
                     /locus_tag="ACGI6B_003246"
     CDS             1959..2765
                     /locus_tag="ACGI6B_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9752662.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCKIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNDKLATVFENRGAKSDVRGLYWLKHTKAPAILIEVCFVDSKADTDYYIKNKNTV
                     AKLIAEGILNKNINNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVVGGGACEKISSMTKENYTMIKGNDRFDTLHKVLDFIDR"
     gene            complement(2811..3023)
                     /locus_tag="ACGI6B_003247"
     CDS             complement(2811..3023)
                     /locus_tag="ACGI6B_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752663.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            3202..4035
                     /locus_tag="ACGI6B_003248"
     CDS             3202..4035
                     /locus_tag="ACGI6B_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752664.1"
                     /translation="MKILFKEAHKMAREIKRKYSNVDYKVQFSLCLSFLNKKGDIKLK
                     ELKGSEKQIIWANDIRTDILNLTNELEKSKIERIKNEDYKIKDMSIEEMTERCKRKFE
                     RIREAISNIENAKFFIDNFRNVLKYNSLNQKAFHINQILKDSQFAKENRNWSFLQGET
                     QASYRLKKVKDYSISYEEAKKIAKEINFNDNWSNEIKENVLEIVEIFDDAVKQATSTE
                     YIEKAKKYIIETLIQKNAQFYRNGFADIKKKQIENYRKIESDERKIEIAYQVFRDGVE
                     C"
     gene            4112..4615
                     /locus_tag="ACGI6B_003249"
     CDS             4112..4615
                     /locus_tag="ACGI6B_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752665.1"
                     /translation="MNIDFSTILDLKVNKENVKMIEKEINKINNDIFDISLKDCANGH
                     IGSVKETVGKSLNIETWGHGEINSKIGIRLRFNFYYSKGDKVDIENIENILIYVNSLC
                     SKNVESDLIANVRPKFYKLEEKYSTRIEKCEIRHEFIGINKQEIKEFLEYLTEIEKSE
                     RTVIYKK"
     gene            4638..5117
                     /locus_tag="ACGI6B_003250"
     CDS             4638..5117
                     /locus_tag="ACGI6B_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752666.1"
                     /translation="MDKMDKKEAKKFLKVIDMNIDKIEEEAIKAFKESCLIKETNDIK
                     IYIDIQGKVEAIAIQAYAKLLDDDKEINIFTSRQTGNNLDDMFGEIYYVNDYEEFENW
                     CENKCESLNWDSYKKFNKENFEEIAERNIDDSTSVFLEELREGIESCKQELQNIVEN"
     gene            complement(5426..5731)
                     /locus_tag="ACGI6B_003251"
     CDS             complement(5426..5731)
                     /locus_tag="ACGI6B_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752667.1"
                     /translation="MNVEQSKSKKVYLNIGEELSKKMTKSELNKFSQELANYLQYAGL
                     DKNMKIKLFQTILEMVARKKITLSSDSEFTEGILQSDEAEMTTNVGFIIEGLSYHND"
     gene            complement(5941..6141)
                     /locus_tag="ACGI6B_003252"
     CDS             complement(5941..6141)
                     /locus_tag="ACGI6B_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752668.1"
                     /translation="MCFKTYVLKRVVNNNQLEITRSFDLDNLICTATEILKDNEIDLI
                     IIDEKNNVVWKNEKSTPQQENA"
     gene            complement(6274..6423)
                     /locus_tag="ACGI6B_003253"
     CDS             complement(6274..6423)
                     /locus_tag="ACGI6B_003253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752669.1"
                     /translation="MFKNKMDKCTHVLTAYISSSYDYYNFLDTQLDDFILEYGENVVS
                     LVYTK"
     regulatory      complement(6477..6574)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6783..7172
                     /locus_tag="ACGI6B_003254"
     CDS             6783..7172
                     /locus_tag="ACGI6B_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752670.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            7266..7649
                     /locus_tag="ACGI6B_003255"
     CDS             7266..7649
                     /locus_tag="ACGI6B_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752671.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            8367..9758
                     /locus_tag="ACGI6B_003256"
     CDS             8367..9758
                     /locus_tag="ACGI6B_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9752672.1"
                     /translation="MRNKIIIGITFLLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(9906..10214)
                     /locus_tag="ACGI6B_003257"
     CDS             complement(9906..10214)
                     /locus_tag="ACGI6B_003257"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9752673.1"
                     /translation="MLREQGSAIRDTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            10251..10772
                     /locus_tag="ACGI6B_003258"
     CDS             10251..10772
                     /locus_tag="ACGI6B_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9752674.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(10901..11137)
                     /locus_tag="ACGI6B_003259"
     CDS             complement(10901..11137)
                     /locus_tag="ACGI6B_003259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752675.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(11447..14875)
                     /gene="uvrA"
                     /locus_tag="ACGI6B_003260"
     CDS             complement(11447..14875)
                     /gene="uvrA"
                     /locus_tag="ACGI6B_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9752676.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMSDQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
BASE COUNT         5690 a         1819 c         2275 g         5128 t
ORIGIN      
        1 aaaataacat tactacaagc attaccattt ttaacagtat cttcttataa ttccattaca
       61 gctacattta aagctgacca cacagaccct acattgctta caaaaataga atattttata
      121 gatgatgtgt taaaagaaac tataacaaca gatttaacaa cagagaaaac aattaactat
      181 acactagaag ataatgcact acacacactt aagatagttg ttacagacag taataatgct
      241 acagcagaaa aagtgttaag tataagtaag aatataatgc cattgcccga agatgtgaat
      301 ctgcaagaca tatcttctaa actaattgaa gttaatgcag gatttaaaac tggaaaaaca
      361 agtattataa acactttagc attaaaaaat atagaagcaa atttaaataa tacattagtt
      421 gaattatcag agaaaataaa aacaggtttt gatagttcag atgctactgc tgaacaactt
      481 caaaatcaga ttactaattt aaataatcag ttaagccaac gtaaaagatg ggctaagggt
      541 acttacactt ttactcaaag tgattatgaa aatttttatt caaggtattc aagtgctgaa
      601 aattatgcta agagtcttac tgttcctatt aatttaaatt tcatccctag tcttgtcatt
      661 gttgaaaaac tttgttttta tggaaatggg tatactgtta atttttatgc tattaataat
      721 ttaaatcaac ttgcttctgt tggtcgtgtt cctaagagta gtggagaata tcgtttttgt
      781 gcttttatgt ttgtaaaaac tattactagc aaagaatttt ctttatatac ttatcgagat
      841 gaatacactg atagtagtag cgtagtatac cgttgtaaag tcggagaaag ttttacatgg
      901 tatgcttatg aataatatga aaggtggtaa tttaaatgga aagaggaaat agaattatat
      961 gtgaccaaac cgggaaaata ttactccaaa ctggagaagc aacaggggat atattagaac
     1021 atgatgaaat aacggaatta cattttgttg atattccgta tggaagtata gactatacaa
     1081 gaaatagaat tataggtata aatatagaaa caaaagaacc aattttagag gaaataccaa
     1141 catttatttc agatgaagaa aagagaatac aagagttaga aaatcaaatt ttattaaatg
     1201 aaaatgagaa agtaggagga attttataat gaatataaat aatgttgtag taagaatatt
     1261 agcagagaga atattaaata agggattaaa tcctctaaaa aatagagtat ttgaacttga
     1321 tgatgttact aacacagagt acaggatggc agttgaaaac tacattatag aacatagtga
     1381 ggtagtagaa ggagcagaac ttacaaaata ggtaatgttc tttttttaat acaaagcaat
     1441 aggaggtttt catgaatgaa gaacttgtga aagaaaaaat aagtacactt gaaacaagag
     1501 taaaagaaca cggcaaacaa attgatagaa tcgagattga gcaagcaaag tttgctatac
     1561 aaatacaaaa cctatgtagt gatataaaaa atctgacagg agtactcaag tggcttgtag
     1621 gagttataat tacaacttta gtagggttct ttatatttgc catacagaaa ggaatatttt
     1681 aattaattag gaggttaaga tatggataat ttaataagtt ttataccaga gcaactgctt
     1741 ttattagtag ttgctcttaa tgtattagga tttggattta agaaatacaa acagttagat
     1801 aataaataca ttccaatcat attgcttgtg ataggaattg gattttcaat atggatgtta
     1861 ggatttaatc cttcatcaat tttacaagga attttatgtt ggggagttgc aataggggca
     1921 aatcaagttt acaaacagtt gaaggatggt gataaataat gaaaatagca atagtaccag
     1981 gacacacttt aagtggaaaa ggaacaggag caactggcta tatagacgaa ggaaaagaaa
     2041 atagaatttt aactgattta attgttaaat ggttgaaaca aggtggagct attgtgtata
     2101 ctggaaaagt agataaatct aataactact tagcagaaca atgtaaaatt gcaaataggc
     2161 aaaatgtaga cttagcagta caaatccatt tcaacgcaaa taaaacaact ctaaatccta
     2221 tgggtacaga gacaatatac aaaactaaca atggtaaggt atatgccgaa agagttaatg
     2281 ataaattagc aacagtattc gaaaatagag gtgcaaaatc agatgtaaga ggtctttact
     2341 ggcttaagca tacaaaagct ccagcgatat tgattgaggt gtgcttcgta gatagtaaag
     2401 cagatacaga ttattatatt aaaaataaaa atacagttgc aaaacttata gcagagggta
     2461 tattaaataa aaatataaat aacaaagaga atggtgaggg aaaaatcatg tacaaacata
     2521 caatcgttta tgatggagaa gttgacaaaa tacctgcaac tgtagttggc tggggctata
     2581 atgatgggaa aatactgata tgtgatataa aagattatgt acctggccag acacaaaatc
     2641 tttatgttgt tggtggtgga gcatgtgaaa agataagttc tatgactaaa gaaaattata
     2701 ctatgataaa aggtaatgat agatttgata cacttcacaa agtattagat tttattgata
     2761 gatagattag aaggtagcaa ctagttagtt gttaccttct ttttttatgt ctacttttct
     2821 ttctttttcc aaacaacttc atatcctatc aaattagcaa tagtcaagat ttcactatac
     2881 ttcatagtct cattatttat tttttgtctt aaattttcaa ctgtattatt cttattatta
     2941 tgttcattca ttaaattatt taactcattc atgctgatat tttcttttac aagaatactt
     3001 ttaatttcat tcttccatgt cattttttcg cacctctctt atttatataa taacataaaa
     3061 aaattaaaag taaaagaata atctacgcta atcaaagtta tatttttgga aatcaaagaa
     3121 aaaactttga aaaagtgttg acttagatat tcatatagat tataatataa ttaacaaata
     3181 aaacaagggg gtaagcaaag aatgaaaatt ttatttaaag aagcgcataa aatggcaaga
     3241 gaaataaaaa gaaaatatag taatgtagat tataaagtac aatttagtct ttgcttatca
     3301 ttcttaaata aaaaggggga tataaaattg aaagaattaa aagggtcaga aaaacaaatt
     3361 atttgggcta atgatataag aacagacatt ttaaatttaa ctaacgaact agaaaaaagt
     3421 aaaatagaaa gaataaaaaa tgaagactac aaaattaaag acatgagtat agaagaaatg
     3481 acagaaagat gtaaaagaaa atttgaaaga ataagagaag caataagcaa tatagagaat
     3541 gctaagttct ttatagataa ttttagaaat gttttaaagt ataattcttt aaatcaaaaa
     3601 gcatttcata taaatcagat attaaaagat tctcaatttg caaaagagaa cagaaattgg
     3661 agttttttac aaggggagac acaagcatct tatagactta aaaaagttaa agattatagt
     3721 ataagctatg aagaagctaa gaaaatagct aaagaaataa attttaatga taattggtct
     3781 aatgaaataa aagaaaatgt acttgaaata gttgaaatat ttgacgacgc agtaaaacaa
     3841 gctactagca cagaatatat agagaaagct aaaaaataca taatagaaac attaatacaa
     3901 aaaaatgcac aattttatag aaatggattt gcagacatta aaaagaagca gattgaaaac
     3961 tatagaaaaa tagaaagtga tgaaagaaaa atagaaattg cataccaagt attcagagat
     4021 ggcgtagagt gttaaaagtt tcaacttgat acaaatatag ttcaccattg aatgctataa
     4081 ttaaaataaa aaataattgg aggtttaaaa catgaatata gattttagta caatattaga
     4141 tttaaaagtt aataaagaga atgttaaaat gatagaaaaa gagattaata aaatcaacaa
     4201 tgatatattt gatataagtc ttaaagattg tgcgaacggt cacattggaa gcgttaaaga
     4261 aacagttgga aaaagtttaa acattgaaac gtggggtcat ggtgaaatta acagtaaaat
     4321 aggaataaga ttgagattta atttttatta ttcaaaaggc gacaaagtag acatagaaaa
     4381 tatagaaaac attttaatat atgtaaatag cttgtgtagc aaaaatgtag aaagtgacct
     4441 tatagccaat gtaagaccta aattttataa attagaagag aaatattcta cacgtattga
     4501 aaaatgtgaa attagacacg aatttatagg cataaacaag caagaaatta aagaattttt
     4561 agagtatctt acagaaattg aaaagagcga aagaactgtt atatataaaa aataactaaa
     4621 aaatggagga aattaaaatg gataaaatgg ataaaaaaga agctaaaaaa tttttaaagg
     4681 ttatagatat gaatatagat aaaatagagg aagaagctat aaaagctttt aaagaaagtt
     4741 gtttaatcaa agagactaat gatataaaaa tttatatcga tatacaagga aaagttgaag
     4801 cgatagcaat ccaggcttat gctaaacttt tagatgatga taaagaaatt aatattttca
     4861 catcaagaca aacaggaaat aacttagacg atatgtttgg agaaatttac tacgtaaatg
     4921 attacgaaga atttgaaaat tggtgcgaaa ataaatgcga aagtttgaat tgggatagtt
     4981 ataaaaaatt caataaagaa aattttgaag aaattgctga aagaaatata gacgatagca
     5041 catcagtttt tttagaagaa ttacgagaag gcattgaaag ttgtaaacaa gaattacaaa
     5101 atatagttga aaattaagta gaagagtatt attattaatg tatatagtat cgcttttaag
     5161 attaactacg agggatttaa aatatttttt aatttattta gaaataatat tgtaataaga
     5221 atttttatat taactacaag ggatttaaat gtcaatttaa tttatagaga aatgatataa
     5281 tggtattttt tagattaact ataagggatt taaatatcaa tttaatctat attcacatga
     5341 tataatatca cattatttta tattaactat gcggaaataa aagcagtgta tttaataaat
     5401 acactgcttt tttaatgcta aagatttagt cattatgata acttaatcct tcgattataa
     5461 atcctacatt cgtcgtcatt tctgcttcat cactttgtaa tattccttct gtaaattcgc
     5521 tatcgcttga taatgttatt tttttccttg ccaccatttc caaaattgtt tgaaatagtt
     5581 taattttcat attcttatca agtccagcgt attgcaaata gtttgctagc tcctgcgaaa
     5641 acttatttaa ttcagattta gtcatttttt tagataattc ttctccaata tttagataaa
     5701 cttttttaga tttagattgt tcaacattca tttttttcaa cccctttttt aagttataat
     5761 tcaattatat tttaaaaatg ctaatacttc aaatataaat atttttgttt aagtatataa
     5821 aacatcaata acattactca acacacctta aaattgattt aaatagctct tttttatgaa
     5881 caaaattaat gatataataa aaaatagaag tgtattggta gtacacttct atagttataa
     5941 ctaagcattc tcttgctggg gagtgctttt ttcatttttc caaacaacat tatttttttc
     6001 atcaataatt attaaatcaa tctcattatc ttttaaaatc tctgttgcag tacaaatgag
     6061 attatctaag tcgaaactac gagttatctc tagttgattg ttatttacaa ctcttttaag
     6121 tacataagtc ttaaagcaca tttatattat tatcctttct cacaaaaata ctttaattaa
     6181 ttttttaaca tagtaatatt taaaagtaga taaaactaaa aagggttctc tagaaatcaa
     6241 tgtctatcta attatactta cttaccaata ccatcacttg gtgtaaacaa gactcactac
     6301 attttctccg tactctaata taaaatcatc taactgtgta tctaaaaaat tataataatc
     6361 atacgaactg ctaatataag cagtcaatac atgtgtacat ttatccattt tatttttaaa
     6421 catataaatt attatacccc taaaaaactt tcataaaaac aaaagaacaa tatctctttg
     6481 gcaactggct gacataactc ataagatatt tatataagtc ttagtcccta tagctttgcg
     6541 tcactaaatt tctctagttt tgccgattta gttttattct acaactaaaa taatacaata
     6601 gaattagtta ttattcaaca aaattatttg aaaatttaat aattttatag atatgaaagt
     6661 tattttcttc taagaaaaca gactaaatgc gtagaatatt tggtaaaact tacctgtata
     6721 aattgaaatc agaaggtttt attattgatt tagattagaa caatttgagg gagtgacata
     6781 aaatgaaaca acaagaagta gttaataaac tacgcagggg agaattgatg ttgatggaat
     6841 acttgtggag gaaaaattct attttatcta aaaaagaaat aatagaagca atgaaagaaa
     6901 aatataagtg gaggaaaagt actacagaaa ttctattaaa aagattagta aaaatgaaaa
     6961 cattaaaaaa gaagaggata ggttttcagt ttagttatga agtgctagta actaaaaaag
     7021 aatatttaaa tgcaataaaa gaagatagaa aggtaactaa atatgatagt ttttttatac
     7081 gaatgtttac cactatgcat aaaaaagaaa agatgacaga agaacaaata aaaaaatata
     7141 tagaaaatat ggaaaaaatt ggaaaagaat aatttatata tatattaatt atttttaaaa
     7201 taaatttgtt ataatattta aatatacata aacatacata aatttacaaa aagggggtgt
     7261 agcttatggg attcaagaaa ttgccagatt ctgagttaaa agtaatgaag tttatttggg
     7321 gattagatgg aaataaggta gcatcaggag aagttgtgga agcaatgaaa caaatttatg
     7381 actggaataa aaatacgaca ttaacaatat tgtctaagct tgcaaagaaa aactatttgt
     7441 attctcaaaa aacaagcaag tgtacatatt atactatcgc tgtaagagaa aaagattatt
     7501 taaaagttga aacaaaaaaa ttctttagtt tttttcataa taattcttta aaaagttttt
     7561 tcactgcttt aaatgatgaa gaaaatttaa gtgatgataa attagatatg ttagaggaat
     7621 gggtgaaaaa ttgggaagaa gatgaataaa atgaatgtga tactaaaatg atactaatat
     7681 gttttatagc gtgttaaaaa atatagaaaa tatagaaata atagaagaaa attaatgtaa
     7741 tagataaggt tttatcagat taaaaagaaa atcaatagaa tgggaataaa atatagaatg
     7801 ccaaaaatcc acataaatac aggatttaag gtctctctta cttctatgtt atactggttt
     7861 gatactatca atatttgcca gtatcttatg gattaataag taaaaaacat cctgctgaat
     7921 gataaattca gcggggtgtt tttattttgt tttagaactc ctttgctctg aaactcgaag
     7981 tatttaacac tgatgatttt gttatctttt agttcctttt gtgtcatgct ctgaaagaca
     8041 aattgataca ataaaaaagt aatttcttaa tcattgatgg agatatgtat ttatgttact
     8101 cttgatggta atttgagtaa tattgttcat agctttttat attcaattaa ttagaaaacc
     8161 atttggtata atcataaata aacttgatga tataatagaa aagatttata attattagaa
     8221 gtatataaaa gtattttatg tctatgaaat gtatgtctat aaaacatata gatgactttt
     8281 gtatattaat gtatgaatat tgatattata gaagaattga gttgtcaatg atactaaatc
     8341 aaatatttat agaagctgga gggattatga gaaataaaat aattatagga attacttttt
     8401 tacttgtatt actaatgtct ggatgtgctt caaaacaaac cgcagaaata aacgtattag
     8461 caactactga tttacatgga gttatacctt atgagttaac ttcttatgta aaagaagaaa
     8521 gaaaaaaaga taaaaatcta actttagtag atgcaggaga tttttttgat agtggagaag
     8581 tgtttggttc tagcatggat agttatttta gtgctagata taaagatggt gataataaca
     8641 ctgaaagtta tattgagacg cctatttcaa aagatatgaa agaggtaggt tatgatgttg
     8701 cagttcttgg gaatcatgaa tttatatcca acaataaatt ttatctagat aatatgatat
     8761 ctgattttga gaagcaaaaa ataaatattt tatcggcaaa tacgtataaa aaaaatggag
     8821 acagttatac aaaaccatac ataataaaaa atattaatac acctgaagga aacgtaaaac
     8881 taggaatttt agggcttact ataaaagaag ttggtgagag gaaacagtgg aaagatggaa
     8941 aacttgttga ctctaagtct ttagaactaa aagaccaaga gggctacaat ggtgaattgt
     9001 atatgaatga tttagtagaa gatgctaaaa agtgggttag tataatggaa aaagagaagc
     9061 ctgacattat agttgctgtg gcacatacag gtgagaaacc taagaaacct aaaaacccag
     9121 gaaatagaat acaagactta gctcaacaag ttgatggaat tgatgctata gttgcaggtc
     9181 ataatcatgt tcaaattgag caacatgatt ataaaaataa atcaggagaa aatgttatag
     9241 ttactgagcc aggaaagcat ggggaatgta tttctaagat taattttaaa ttagaaaaaa
     9301 acaatgataa atggaatata gttgacaaat ctagtaaatt aattaaattt gaaaaaaatc
     9361 aagaaagtga taaatttgga gaatttattt cttcaattaa taaagtaagt aaagataaga
     9421 aagaaataag attatcagaa gtaaagactc ctgaatggga taaaatatat gtttttaaat
     9481 ctggaacatc tgcagatgat atatataaaa caataggata taaatggaga aatatatcat
     9541 ctccaaagag tgatggcatg gtgcagattg ttgtaatgaa taataataag cctgttagct
     9601 atatatatgg tgaagctgaa gttatgccaa ttaatataaa atgtgatgaa tcagagtata
     9661 aagataatgt tgtaactatt tatcctaata aaaatgataa atttaaaatt gaaaaaggta
     9721 aacaagaata tagtattaat ttaaaacata tacaatagaa acttttatct tatttaagtt
     9781 tagattgtac ttaaaataaa gtgtgtatta ttttaaaaat tgatgataat taaaatcaca
     9841 acattaagga atagtacaca ctttttatgc taaaaaatag aatgttaaaa aattaaacct
     9901 ttaaattaaa gtacattgct tatattatcc aatattatct tcaaaggttg tgtaatattt
     9961 ttatccttat gtaatattgc taacatttta ttttccatat ctatcccatc taattcaatc
    10021 aatcgcaatt ttttatgtaa gagtgaatct aataataaat tttctggcaa aatggttata
    10081 cctaaccctg cttgtgcagc tttaataagt gcaaaagaat taacactttc ccaaacagga
    10141 taagcttttt ggtttgaaag agataataca ctgtccaaag tatcgcgaat ggcacttcct
    10201 tgctcacgta acaataacgg aaacgtgcat aactgtcgta gagataaggc ttgtttagta
    10261 ttggtggtgg ttatgcaatc atacctttaa tccaagagaa agtagtaaat caaactggat
    10321 ggataagtga gaaaatattt acagatatta taactatttc acaaatgaca ccagggccac
    10381 tggctgttaa tacttctact tttgttggac ttcaaattgg agggttttgg ggagcagttg
    10441 cagccacttt aggttgtgta ttatgtggtg tagtaatatc tctattttta tataattttt
    10501 ttcaaaagca tcagaactct acttatatat ttgaaatatt aaatggtcta aaatcagctt
    10561 cattaggttt aattatctca gctgcggcta caattataat tcttgcatta tttgggacga
    10621 ataaagtgta cttcaattct atatttgaga tgctaaactg gactgcacta ataatctttt
    10681 ttgttacact actgatagta cgtaagtgga aaattaatcc tattgtgatt ataataatga
    10741 gtggtatact gggaatattt ttttatttat aagtttttgc taagtaaagg atatctattt
    10801 ttttatatct taaatccaaa tagccaataa agtaaggcta tttggatttt taataaattc
    10861 tgaaaagata atcagaaacc tatataacat ttaaattata tcactggttt tctacaaacc
    10921 aatcataagc ttcttggcaa aatttcttac tatctatata gtaactatta tgaacattat
    10981 taatttcaac aagtttcttt ttacatttta gtgaattata atagctatca aaatattggg
    11041 ttttaaaata tctatcatga agtgttttaa agacaagagt aggtatgttg actccatctt
    11101 ttatcttact atcttttgca ataaagcatt ctaacattgt attaattatc tttgtactat
    11161 ttttccaaat atgaggattt ttcttttcaa atattttata aaattcatta tcatagactt
    11221 cattataacc tttatattta ggtggtttta ctgttatgtg aaaattagga agaaacttaa
    11281 agaaaaaata taataatttt tatgttctgt tttatatatt attcatattt tatcacagat
    11341 aatatactaa agttaatttt tttattgtac aactattata ataattcttc tataatataa
    11401 gacattctat aattttatat ttaagttcat gtgtatttaa ttgtttttat ataaaatgtc
    11461 cagtaatact atttggattt atttttattt catcaggagt tccagaagca acaatttccc
    11521 ctccatttat tccactgtct ggacctatat cgataatcca atcagaagca cgtataacct
    11581 ctgtattgtg ttcaataatt aaaagtgaat gctgttcatt tacaagtttt ctaaaagtaa
    11641 ttaacaaatt ttctatatct ttaaaatgaa gtccagtagt aggctcatcc aatatgtaca
    11701 gcattcgttt tttagttttt gtccctaatt cttttgctaa ttttatcctt tgagattctc
    11761 ctccacttaa tcctaatgta ttttgtccaa gctttagata tccaagtccc acttcatcta
    11821 ataaggagag ttttctatat atatcagagc aatcgctaaa aaatgttttt gcttctgcta
    11881 cttccatgtc aagtatatct gcaattgtct ttcccttgta ttgtatagac agtatttctt
    11941 tttgatatct cttaccttga cattcactac aagttaccca ttggtctgac atatattgca
    12001 gaataatttt tgttttacct aatccttgac agtttgggca ttgccccttt ttgctattaa
    12061 agctaaaata gctttcatcc aataattttt gttttgcttg tggagtttca gcaaatagag
    12121 aacgaatttc atcaaaaata ttgatatatg tagcaggtgt agaacggcta gattttccaa
    12181 taggtgcttg gtccattaag ataaaatcat caatagcatc aaatccagta aattcagtat
    12241 aatttttatc tgggatagat ttttgtttca atttttcttc tagagcagga agtaaactgt
    12301 gaaatacaag agagctctta ccagaaccac taacacctgt aatagagcac atacagttta
    12361 agggaatttc tacatcaata ttttttaagt tatttgcatg gcatcctttt aagcttaacc
    12421 atttacttgg tataatttta ttttgtgaac ttattttatt gtatgtactt aaatatttac
    12481 ctgttataga gtttggattt tttataattt cttgggttgt accttctgcg ataatgaacc
    12541 ccccttttgt accagcactt gggcctacat caataatata atcagcagac agtattgtat
    12601 ctttatcgtg ttccacaact ataacagtat tacccttatc tcttagctct tctatcattt
    12661 tgatgattaa attatggtct cttgggtgta aaccaataga tggttcatct aaaatgtagg
    12721 ttaagcctac taattcactt cctagctgac ttgataatcg tactctctgc agttctcctc
    12781 cagataaagt tggagctgtt ctatctgttg atatatagct aagacctacc tttaatagat
    12841 aactaactcg aatttttaat tctgttaaga tatcatttac caaagagagc tcattttttt
    12901 gaaggtgatt tggtaattca cacaaccaat tccataactg ttctatcgtc atttttgtta
    12961 gttcagggaa gcgataacct ttgattgtag tgaatctagc ctcaatgcac agcaattcgc
    13021 caccacatgt attgcaagga attttgttca tatattgatg caaggtatta ttttcagaac
    13081 tagattctct aaaaagtctt tgaatgtggt tgattgctcc tgaagcaggt cttctaatct
    13141 ttgattctcg tccctttgat tcaaagctaa attcatatat cttatcgtct gtaccatata
    13201 agattgcatc tataaaatct cttggtaatt cattccaagg tatatctagg tctatatttt
    13261 tatcttttgc tattgcatat aattcaccta ccatccaatt accatttggc tttttccccc
    13321 tcaattttcc aaaatagtaa gtagctccat ctaatattga aatagttggg tcttttacaa
    13381 ttaattgagg attaacttga taggtaaaac ctaatccatt acaatcgtga caagctccag
    13441 tatgggagtt agcattgaag gttgaagtat ttatcttaga aaaaatagtt tgacaatgtg
    13501 gacaataatt ttttgtatta ttcttttttt ctaatggaat tttgcaatat ggacagtaac
    13561 tttttccgat tgatacaaat aacattctta aataatccca aatgcttgta agagtaccaa
    13621 cagtagagcg aggattttgg cttccttttt tctgttcgat ggctatagta ggggttaatc
    13681 cctgaagtaa atcaaattcc ggtttttgaa gctgttctga aagttttgct ccgttactta
    13741 gattttcaaa ataacgtctt tttccttctt cataaatcgt atcgaaagct aaagaagatt
    13801 ttccactacc acttacacct gtaataacag ttatttgatt ttttggaatt gtaacttcaa
    13861 catcctttaa attgttttgc cttcctcctt ttaaatagat agagctttta ttatgacaaa
    13921 gtgaattttt aattgattta tttattgctt ttgtaaattt gaatttcttt tctagttcaa
    13981 gaggattgcc taattctttt atttccgaaa tacctaatat ttttaactga gaaaagattg
    14041 atttcaaata atcttcaggc aatttttcta ttaacaaatt aagggcttct atttttatat
    14101 ttccgcttga taatatctct gccatttcat ctaataaatt tttttctgct cttgttaatt
    14161 gattgtattt tttggaactt aattctgtcc ataaatccca tggaagcaat tctaaaccaa
    14221 tcatattatt aaagtcatgt agaagattac cctttacaga atgccaacct ttccaatttt
    14281 tattatatcc aaagtcatct ggatgagcat gtccttgccg acatcttatc caggcttctg
    14341 aaccagttaa aaaaccatct gtttttccga catcatgagt gtcaaaatta atttgaaaat
    14401 aatttttttg tatgtcatct atttgtgcat ctattcttat ccatctcttt gttagagtat
    14461 catagtattc tacaagccag tggtcttcat aagtaagctc actttcaaaa taattagcaa
    14521 aacctgctct cattctagcc tcaaaacctc gatatcttag gagcgataca agaagtaaag
    14581 aataatctct gcacattcca acaagccttt gttttgggtt tcttggaata gttagtgggt
    14641 tagaatttat ttcaaatatt ctttttaaca tttgaggaat tgtacgaagc aattcctcat
    14701 cagattgttg cttattaaag gatataccat ataattttcc ttggtctcca tgaattacta
    14761 ggctttgaac cacttttaat aactcaattg gagtctttgg taaatcttga tatttatttt
    14821 caaatttttt aggatttgtg atgatacttt gatttttata gttatttatt ttcatttata
    14881 tttcacctct ctaaatggat gatataccct ga
//
LOCUS       DAYAOY010000105        14488 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYAOY010000105 DAYAOY010000000
VERSION     DAYAOY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.105"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..542)
                     /locus_tag="ACGI6B_003261"
     CDS             complement(<1..542)
                     /locus_tag="ACGI6B_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFG9752677.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTIND"
     gene            complement(813..1589)
                     /locus_tag="ACGI6B_003262"
     CDS             complement(813..1589)
                     /locus_tag="ACGI6B_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFG9752678.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(1646..2617)
                     /gene="asrC"
                     /locus_tag="ACGI6B_003263"
     CDS             complement(1646..2617)
                     /gene="asrC"
                     /locus_tag="ACGI6B_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HFG9752679.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(2630..3421)
                     /gene="asrB"
                     /locus_tag="ACGI6B_003264"
     CDS             complement(2630..3421)
                     /gene="asrB"
                     /locus_tag="ACGI6B_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HFG9752680.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(3422..4429)
                     /gene="asrA"
                     /locus_tag="ACGI6B_003265"
     CDS             complement(3422..4429)
                     /gene="asrA"
                     /locus_tag="ACGI6B_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HFG9752681.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(4820..5509)
                     /locus_tag="ACGI6B_003266"
     CDS             complement(4820..5509)
                     /locus_tag="ACGI6B_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9752682.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(5572..6420)
                     /locus_tag="ACGI6B_003267"
     CDS             complement(5572..6420)
                     /locus_tag="ACGI6B_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9752683.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(6439..7326)
                     /locus_tag="ACGI6B_003268"
     CDS             complement(6439..7326)
                     /locus_tag="ACGI6B_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9752684.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(7384..8187)
                     /locus_tag="ACGI6B_003269"
     CDS             complement(7384..8187)
                     /locus_tag="ACGI6B_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFG9752685.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(8347..8799)
                     /locus_tag="ACGI6B_003270"
     CDS             complement(8347..8799)
                     /locus_tag="ACGI6B_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFG9752686.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(8929..10431)
                     /locus_tag="ACGI6B_003271"
     CDS             complement(8929..10431)
                     /locus_tag="ACGI6B_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFG9752687.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(10464..11345)
                     /locus_tag="ACGI6B_003272"
     CDS             complement(10464..11345)
                     /locus_tag="ACGI6B_003272"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFG9752688.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            complement(11415..12086)
                     /locus_tag="ACGI6B_003273"
     CDS             complement(11415..12086)
                     /locus_tag="ACGI6B_003273"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFG9752689.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(12359..12850)
                     /locus_tag="ACGI6B_003274"
     CDS             complement(12359..12850)
                     /locus_tag="ACGI6B_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9752690.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(12872..14236)
                     /locus_tag="ACGI6B_003275"
     CDS             complement(12872..14236)
                     /locus_tag="ACGI6B_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9752691.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
BASE COUNT         4737 a         2596 c         1351 g         5804 t
ORIGIN      
        1 tcatcattaa tagtattttt taaaagacta attttttcta ataattcatc acatatagat
       61 atcagttctt tatctccgac atttttcatc ctatttctat tcataacaaa ttgagcaatc
      121 attattataa ctgctccaac agctaaagcc gacaatctca taggaaattc actttttcct
      181 acaggtatac ttaccataaa tatatacatt agaccaaagg gtacatatac tgctcttttt
      241 aagtcataac taaatacata ccctattata aataatgcta taaaatttaa taaaactcct
      301 aaatacatat tttgtactgc aaaaaatgat agtattccta agattatatt tattgctaaa
      361 tacttcagta aatttttaat tggagaaata gttaaatctc tttccaagag cgttagcata
      421 gctgttacta tacctacacc tataagtgta tttttacttc caaataaata tataaatata
      481 gaaataaatg ctattataaa aataaacaac tttgtttttg atattactag tttctttgtc
      541 atcatatccc ccctaaaaat ttcaacaaga ccaattttat catatttgtt atcttattca
      601 aaatgcgctt ttttacggta ggactttaac tttttttata tttagaacta tataattcat
      661 atataaacgt attttttata tatataaccg atatattttc gtatgctccc atattttttt
      721 tgtttcaaat attttttcaa aaatataaaa ataactctct caacaatact aaaaatgcat
      781 ttatcaagag agttataatt tatacaataa atttaattaa gcttttcttt acctaatata
      841 taagttccaa gacccataaa taaagctcct ccaacaatat ttccaagtgt tacagctact
      901 aaattataaa tagctcctcc aatagtaact gtatttattg ttggtgaaaa taatgaaaca
      961 ctataaattg tcatatttgc tacactatgc tcaaagcctg atgttataaa agcaaataaa
     1021 cataagaata tcataattat ttttgcagta tcactattag ttctaaacga acacaacact
     1081 gatacacata ctagaatatt acataaaatc cctctaaaga aaagagctgt aaatggcatg
     1141 gatgctttac tagctgctgt atttgcaaaa aattcagcta caggtccttt gtctattaga
     1201 ccagttccta caaatataat gcctaaaacc aaagctccga ttaaattacc tacccaactg
     1261 tatgcccata ttttagaagt atcttttata cttacacctt tatttaacat accagcagac
     1321 ataaccatat tgtttccagt aaataactct gttcccgtca ttataaccaa acttagtgct
     1381 attgcaaatg ataaccccat tactatctta gtcataggtg aacctgcatc agtcaaaagt
     1441 cctccaatag taaatatcaa aagtatacct atacctacat aaagtcctgc aaacgcagaa
     1501 gataccaaat attttacttt acttgtgttt aataaattta tcttatttat agctgcatta
     1561 gttaatttat ccaaagtttc tttatgcata attccctctt ccttctatta aattttaatt
     1621 tattcaaata tatgttttac tcaatttata gatatttaat tccactccag tatatgtttt
     1681 cgtacatttt agttatttct ggaagctcta caccttcaag tgcccatttt ttaaattcca
     1741 taaatcctgt tctatcaatt atatatccta tatgttcttt tcctcctggt gcatctttat
     1801 ctatatactt ttctacatat ctataagtat taagtattat ctttattatg ctgtcctcat
     1861 ctacccataa aagaaaatct tctgctagtc tagggttttt ctttcctgtt ctacccatta
     1921 tagctaatct atagtatttt ttctcatctc tagtccatgc atttgttggg caatttagca
     1981 cacactctcc acagcctata cacttatctt tatctcttac caccttatta ttttctgttt
     2041 ttaatgctcc aacagatatc ttagaacatt tttttacaca tgctccacaa tttacacatc
     2101 tatccttttc atatattggt aaagccatac caataattcc aaagtcattc attctagctt
     2161 ttatacaatc atttggacaa ccagttaaag ccactttaaa atgtaaatca tttggaaata
     2221 tagctttttc tattctttta gcaaattcag ttgtattata ttgagccttt ggacagactt
     2281 tatttcctat acaagctgct atatttcttg tacctgctgc tggataacct gcgtttttct
     2341 cactttgatt tatattcatt ttttcaataa caggttgtat aattttattt atttcttcca
     2401 tatcttccat atttattcct aatatttcaa acccctgtct tgtagttatg tgcacctgtc
     2461 cattgccgta ctcacttgca attttagaaa ctacagctaa actctcaggg tcaataagac
     2521 ctccaggtac tctaactctt aaagaagtct catattttgt ttttgtaatt ctataagcat
     2581 ttttcattac ttttttggta tttaaatctc ttatcacaaa cttcaccccc taatcaataa
     2641 gatttttagc atatgaataa tcaaatactg gtccatctaa acatatgtaa gtatcatcca
     2701 ttttacaatg tccacattta cctattccac aacacatttt tctttcataa gaaacccaaa
     2761 tgttcttttc atctaaatct ctttttaaaa attcttctac tgtaaatttc atcatcattg
     2821 gaggtccaac tacaactata gaagtattgt ttatatcttt catatccaat tctggtatgt
     2881 attttgttac taatcctaca ttaccactat atccttcctc tgctccatct acagtaacta
     2941 aaatatcaag ttttttactc catctcttta aatcatctgc aaataaaata tcttttggtg
     3001 atttaaatcc tactattaat ttaaactttt cgcatttttc tttattatta taaaaatatt
     3061 caactattcc tcttacaggg gctaatgcac ttcctcctgc cactactaca atttctcttc
     3121 cttcataaag ggatacatca aatccatttc cataaggacc tcttataaaa aaagaatctc
     3181 cttctttata attaaacaac tcgctagtta cttttcctac atttcttatt gtaaagtcaa
     3241 tataatcctc tccatatcct gatactgata ttggactttc accgtattta ggtatagata
     3301 tctcataaaa ctttccaggc aatacacctt ttgtattgca gtttactcta aaagtccatt
     3361 ctttatcagt atgtttaact atatctagta tttttgcact tactggtata tatgggttca
     3421 tttatacttc ctccttacta attactaaat catttatttt ttctatacac tttgaaaatg
     3481 atatgtattg cggacaggca tcatcacatc ttccacagcc aacacacata tgatatccga
     3541 atcttttttt aaaatcatgt attttatgca tagttttaaa tctcattctt tcgccttgtt
     3601 tttttctaaa agaatttcca cctgctatat ctgtatatcc atctacttga caagaagccc
     3661 aaacccttct tctctcacct acattttcgt tttccttata gaaaatatcc tgcattgtaa
     3721 aacatgaaca tgttggacat acaaaattac atctaccaca tcctatacat cttgtatcat
     3781 attcatccca tattgaattt cctatcattt catttgaatc aatattctct ggcacatcta
     3841 cagatattaa attatcagtt acataatcaa cattaaattc agttgcttct ccattaaata
     3901 cttcaaattc actatccttt atatctaagt acagtgtatc tccttctata tttaatccaa
     3961 ttgaataatt atcagttttg ttagaatcca tgctcacaca aaaacagttt ctaaaacttt
     4021 ccttacaccc tacaagtgca aactttactt tatctctgat atttttataa aaataatctt
     4081 tttcttcacc attatttaga tatatttcat caagtctttt tataccattt atgtcacaag
     4141 ctcttaagaa aattaaaatt ttcttgttgt ctaaatcgct ttccttaaac tctttttcag
     4201 taaaataaaa caatacttga tttatgggaa gtacaacttc ttttggtgaa aagtttgctt
     4261 tcttagcaaa ttccatttct tcaaaagcgt ttacctcctt gtatcttata acatcagtat
     4321 ctgaaaaagt acctttaaat gggattaaaa ctggtgcaaa tatcttgtag tcttttttta
     4381 gataattcag accttcattg aacttagatg aatctaattt tagtttcatt tttatcacct
     4441 ttcttatatt ttagttttac ttacttgcta tcttgtttaa atcttaacac tttattttct
     4501 atgcttccgt gataattatc acagtttcaa aaataataaa aaaaatatta atggagttta
     4561 ttaagttctg ttaatatttt tttattatct acattattcg atttcaaatc taatcactta
     4621 aatacaatca ttaagtacaa taaaactact atctactcaa ttttttgaat aaaaaagaat
     4681 cttatttgtt tttttataca attcaatagg gtaaaataaa aattaataaa ttagacaata
     4741 aaaaagaatc tataaataga ttctcttaat aatattttta tttaattaaa attctaataa
     4801 tactcttact taatcataat taattatttt taaaatacct tgataattca gtttctttaa
     4861 cataaaaacc tttattgtca attttaataa gttcttcttt ttgcaatacc tttatcattc
     4921 tggaaacagt ctccctttta cagccaagca tatcagctaa atatgtaact gtaatattta
     4981 catttagtaa agtccattct ccacagcaaa ctccaaattc tctacttaac ctatataatt
     5041 ttgctgcaag ttttttatct acttttatgg atatagaatt tttcaactgt ctatataaac
     5101 gtcttattct tctttccata taagaaataa tatcctttgt aagttgaaaa tcattttgca
     5161 taatttctaa aaattcttca atactataat aagctacaat tgaattttca aatgcttcac
     5221 agccaacagt agagttttta gaagcgtcaa gtgtaatatc atttatgact tccccactat
     5281 ttaatatgaa aattactttt ctctctccat tttcacttat cttaaaaata gatacctttc
     5341 cttctaaaag tatataaaca ctgtctaagc tatctttttc ataaaataaa atttcttttt
     5401 tttgtaatct ttttagttta actttttctt taagttgatt cttcgtttta gtacttaaat
     5461 tattaaaaat tattacttta tcaatattat aatcttttaa tttagacatg taatatctct
     5521 ccattcctaa atttttaaga gagctctata aaacattaca aatttcttat tttattcatt
     5581 tttaaacaat tttatagcat ttgtagtttt aattttcctt tcaatagctt cattagaaag
     5641 ttcttttact acctgtgtat atactaaatc aataatgaaa aattgtgcta atttagaaga
     5701 tattgaacca gtttccagta ctgtttctcc cgatacataa gataagtgta catctgaaat
     5761 atcctttaat tcagaatttt tattttcagt aattgatata attttagcat tattggctct
     5821 tgcaagcttt acagttttta taatctcttc tgtttcacca gaatgtgaaa ttgcaattat
     5881 taaatctcct tcttccatta tggaagacat cattatcatt gtatgactac tgtcaaaact
     5941 tacacaatta agacctattc tcataaattt atagtttgaa tcctgtgcta ttataccaga
     6001 atatccaata cctatgaagt aaactttctt tgcatttatt ataagccttg cgcagtcatg
     6061 tacatcctta ctattgatta tctctactgt gttctctaaa gtggttacat ttgaacttaa
     6121 aagttttttt gctgtatcca aagctggctc atcattttgt atatttttat ttataatatt
     6181 ttttttattg atagttgata tttcttgtgc taaagtaact ttaaaatcct gtaatccatt
     6241 aaaattcatt ttttttacaa acctagttat agttgcttca ccaatattgc tctctttagc
     6301 tatttgtgat attggtttat aaaatacatc atctatattt tcttttatat aagctattaa
     6361 taatttatct gattttgtga ctttaaaagt tggtgattcc aattgttcta aaatactcat
     6421 taaaaaccct tctattcttt atattttgtt taaaaaatta tataatgctc caagcattcc
     6481 tgcatcattt tgtctctttg caaattctat attagtgttt ttgtaaactc tctctataag
     6541 aacctccttt aatgctttgt ctatttttgg ttttaggaac ttttcttgtg ccataatccc
     6601 accacctaaa attaccactt ctggatttat tacataacat atatttgcga ttccaactgc
     6661 taatgatttt atcatattat cgagttcttt taaacaatct tcatcattat tctttgctaa
     6721 atcaaaaatt atttttccat ttaagtcatt ttcattcaaa ttttttattt ttgctacttt
     6781 tttaattaga ctgctagtag atgccaattc ttgaaaagaa cttccattta cattcatata
     6841 tcctacttca ccagcactat tactaaaccc atttatcagt ttattattta ttataataca
     6901 tccaccaatt ccagtaccta ttgttaaaca tatagaagat gttgcatctt tacctgctcc
     6961 aagccacatt tcaccaagtc cagcacaatt aacatcattt tcaacttcac attttatatt
     7021 aaactctttt tctacttctt tttttaattg catgccctta tagtttggaa ttaattcttc
     7081 taatgcaaat agtattttac cttcaactgg gtcaaccata cctgcagttg atatacatat
     7141 accacttatt tcattttcct ctatatattt ttttactata tcttttattt tggctaatat
     7201 tccttctcca cctttttcct ttgcttcagt atcaagactt gatttatcaa ttataaatcc
     7261 atcttctcta ataaaaccat attttattga agtaccccca acatctatac aaacgtaatt
     7321 tttcataaca tatctccata caaattattt ttataaatta acaactctat ttaaaatttt
     7381 atattacaca aatcttttac ttatcttttc caattcacag atatatttcc ttgcttctga
     7441 taagtctata gtatcactta atacactttt ttctttggtt atatgatact tataaatatt
     7501 tttacataag tcctctccta acatattaag accttcttga gtgtaatgaa catcatcaat
     7561 agttaatcct ctgtcatgaa actgcatagc aattttgctt cctacacata atatatcaca
     7621 ttcactacaa gccgactcct gtgctgctgc aattcgtctt gttctaataa aatattcatc
     7681 ttctccaagc caataaccag ttaaacttac aaacatttta tcaatcatac attcttcttt
     7741 aagcctatac gcaatttttt ttagactttc tttatagtac aatacatcac taccatattt
     7801 tgcatcactt tcaccttgaa tccaaataac gtatttattt tctatattat aatgtttact
     7861 taaggtagta cagcctgtat taaatttttc tatagtgtca tttagaaacc agttattatc
     7921 atgattccag ttttttattc ttgaaccatc ttttgcattg tgaataaaac aaactttatt
     7981 tccagtaagc ttattccatt catttgaaaa tgcaggtgct atagtaccat caccttctga
     8041 agtctctagc tcacatctca tatttataat ttcatcagta ctcaaatatt caaaacaatt
     8101 ttcatttgga atgatgctct cttttggatt tcccaatcct ctagcattgc tttgtcctat
     8161 taataagaat acatctatat tttccataag ttaactcctt tacaaaattt taagctttta
     8221 attattttca attttctcat aaaaaactaa ggctgaaaat agtaatctaa ctaaaattta
     8281 cgaagtctca ctaatcgctg aaagttaaat cactattttc agccctagtg gttatataaa
     8341 ttatcattat ctaaccaaaa ccttaaaaat agcttttttg atattttctt tttctaaaac
     8401 ttttattcct gtcatatgag catcttccgg ataacaaact aaaaaatcac cttttctaag
     8461 ttccatgtat ccattatttt taccatcaag aggcaagaaa tcgccctttt cttcatatat
     8521 tttttgttct aaattttcta taaaatttat atctattcgt tcacatccat caagcatcaa
     8581 atgaacatct atatacttct tatgagcttc ccagaatcta tcttcttttt cacaagtttt
     8641 atactctaca atattcacaa aaatatcatc accatctatt gtataagtac ctttttcata
     8701 atctattaat gaattatctt tagcatattc aaaacatcta agtaaatctt catgcaatgt
     8761 tgaatatgtc ttactatgat ttatatttcc aaatatcata agcactatct ccctatataa
     8821 ttataaagtc tatttttata cttttatata caaagtatat tatacaatct tatagagtaa
     8881 ctaaattatt tatctaatca ctctataaga caatttatca tttaaatatt atttttcata
     8941 ataaacagta gtctcagctt ttggtaaaga tacttttgct ttaactttag cttctattgc
     9001 actaactatt gtacctacca ctacagaagt cgtgattgtt attagagaat atgcccaaga
     9061 agttacttct ggcatacggt attttaaata aacaactaaa attgctgata ctatgaatcc
     9121 aacatatgct cccattttag tcgctttttt acaaaatgca ccaagtacaa atattcctgc
     9181 taaaacacct agtactagcc ccataaaact attaaaccat tcatatgctg actttatttc
     9241 tccatttgct aatactattg caacagctat agaaagtata cctacaccta atgatatata
     9301 ttgtgctatc ttagtttgct tttccatact tatatctttt gttatgatag tttgtatatc
     9361 taaagtccaa cttgtagcaa ctgaattaag ccctgtcgat aaagttgatt gagaagctgc
     9421 atatatcgct gcaagtagta ttcctgttat acccactggt aattcataag ctatatatga
     9481 tgcaaatact tggtcttgtt gaactgtttg agctaaatct gggttttgag tatagtatat
     9541 aaataaacaa gttccaatta gataaaatac tgttgcaagt cccattgata aaacaccatt
     9601 tccatatgtc attttattaa gttgctttat atcagttgtt gtagtaaatc tttgaactat
     9661 gtcttgactc gatacatatg atgaaaaagt attaagacca gctccaacaa atattataaa
     9721 tgcacttgta gatagtatat ttttatcaaa cataacttca ttacttgcta agaacttgtg
     9781 tccagttgta agagtatcca ttactgttcc aaaaccacca tttatacttg ctattaaata
     9841 gaacaatgtc cctgtaacac ctactattaa taccattcct tgtataaaat cggtccacaa
     9901 gacagccttt aatccacctg tatatgagta aattattgct attacaccca taactattat
     9961 cagtacattt acaggtattc ctgtaagacc agccagaact attgatggaa gatacattat
    10021 gatagacata cgtcctattt gatatattat aaacatcaaa gctccaagta ctctaagacc
    10081 tttgctttcg tatctcattt ctaagtattc atatgctgta tctatttcta acctactata
    10141 tagaggtaag aaaaatttta ttgtcaaagg tattgctata aacataccta attgtgcaaa
    10201 ccacaaaatc caagtaccac cataagaatt tcctgctaat gataaaaatg atattggact
    10261 taataatgtg gcaaatattg atactgatgt aacccaccac ggaatagttc catcaccttt
    10321 gaagaactct tttcccttca tttccttttt tgaaaataac aatcctgcaa ataaaactgc
    10381 caataagtaa accactagta ttgcaaaatc tatccaagta aatcctacca tttgactaat
    10441 cctcccatac taaaagtatt ttattaaaaa tactttctgt atatttcttt tgctcttgat
    10501 ttcatttcat cagttgcttc tttcataggt tgtctacagt atccagcctc aacaccttgt
    10561 tcttcaagta gtaatttaat tgtttgatat aagccatttc ctaatatatc tgttattaaa
    10621 tcattagtca catgttgtac ttcaagtgct tctgatattt tttcattctt agttaattcg
    10681 aatatttgtc tagctctgac accgtttaca ttgaatgtag aaccaattgc cccatctaca
    10741 cctaaaactg tagctggtaa catcatttca tcaaatcctg caaatattaa tttatttggg
    10801 aatgtttttc tcattctctc taataagtag aaatctgctg ctgtaaactt aacacctatt
    10861 attttttcat tttcaaataa ttctccaaac tggtctaaac tcatatcaac accagttaaa
    10921 aatggtatag agtatattat taatctatta tctacagaat ttattattgt attataatag
    10981 tgttttattt cttcaaaatc aaatttatag taaaaaggtg taacagcact tattgcatca
    11041 tatcctaaat cagttgtaaa tttagcaagt tctacagctt cttttaaatt aactgaacca
    11101 acttgagcta ttaattttat ttcttctttc acttcatctt tagctatttc aaatattctt
    11161 ttcttttcat cagttgaaag catgaagttt tcccctgtac ttcctcctac ataaagacca
    11221 tctactttac aaacatctat attgtgtcta attatttgtc ttaacccttt ttcatttata
    11281 tttccttcct tatcaaatga aactaataat gctgaataaa ttcccttcat atctgttgtt
    11341 ctcatatctt tacctccaat tttaattttc aaatatagtt aaatataata attttttaat
    11401 ttataaatta atttttaatt aatttcaatt gctttaacaa atttttctgt tattaattgt
    11461 ggtcttgtta ttgctgaacc tactactgat gaatgaacac ccaactcaaa gacctttttt
    11521 aaatcttgag gagtatttac ctttccttca gctattacag gtatttctaa atctttaact
    11581 aatctttcca ttaatacaaa atctggacct tctaatgttt tagtatatgg agtatatcca
    11641 gatagtgtag ttgaaacgca atctacaccg tactcttgtg ctttgatagc ctcatcataa
    11701 tttgaaatat ctgccattac tagaacaccg ttttctttta tatactttat aagttctttt
    11761 aagtcttcat tatttggtct aactctattg gtagcatcta atgctatcat ttcacaccct
    11821 gttgttaaaa gttcatcaac ttctttttta gttggcgtta tatatatatc tgaatcttca
    11881 tagtttctct ttataatacc tataacagga agtccagtaa ctttttttat ttcaataata
    11941 tcttcaactc cttgagctct aattccaaca gctccacctt ccatagcagc cttagccatt
    12001 ctccccatta taaaaggact atgaagaggt tcatcttcta aagcttggca tgatactatt
    12061 aatcttccct ttaccttatc tagcattttt taaattacct cgtttctgaa atttattttc
    12121 ttgatttatg tattaataat aacatgaaaa ctttttcttt gcaatatttt ttcatatttt
    12181 tttctcgcat ctatattttt actgatttat tttatatatt agcttgtttt ttacatttaa
    12241 aagaaatttt atttcatttt tttggtgttt attttcattt tatataacta ctgttataag
    12301 tccatttaaa taatttcaat aaaaaaaccc atgtatacta tacataggtt tcccaatatt
    12361 attctaaatc cattataatt tttcttccac agcatggaca aaattcagcc tctgtatcta
    12421 tctcactttc acaataacca caaattataa aatcatcaca ttcattttcc aattcaaatt
    12481 ttttaccaca ttcagcacaa tatttatcat ttacatttcc aacataacca cactcacaag
    12541 ttacattact ttctgccatt tccatttcat taatttccat atttttttca taaattaact
    12601 tatcaagttc tagtaactca tcacagaatt tatcaaaact attatcatta atagaatctt
    12661 ctctaatttt ttgatatgtc agtattccca tttccaagaa tatctttgct ttcttttctt
    12721 gaatatcatc aatctctcta tttaattttg atttttcttt atataattca actttaaatt
    12781 ttccactacc aaactttttc ttaaccttag aaaatttatc atctacttta ttattagtat
    12841 ttccttccac agaaattccc tccaatcata tttaatcagc tgaatttagc ttatatccta
    12901 ttaaagatat cacaaaacta tatacaaatg aaattacaat agcttgtaag actttaaatt
    12961 gcattattaa agttgttcca taattagttg tattaaagaa attaatatta ctatctagta
    13021 taaatgtact aaataaagcc aatattccca tcaaaaatgc ataatatata ctaaatacta
    13081 ttataggtct tatactatct gtattatact taaatcttag cttgtatcca tttaataata
    13141 atattaacat agacaatgct cccattgcat aaaacattaa tttagtatct ccaaataaag
    13201 atgaagaact taaagtaaac attgatactg ttgagtttat tatggttact gaaattccat
    13261 ttgcaaaagc ccacatataa ctagctattt gtgggagtat agtaaataaa tttaatccat
    13321 ttgaataact actcatatct agttcattta agtaactact atcagatata gttagtacca
    13381 atagtattac taatactaaa acataaccta atatgaatgt tcttatagaa tttgaaaaaa
    13441 gagataaata catattttcc ttttcataac tttttttata agtagttatg tatgtacata
    13501 taaaccctaa tacaaatcca tttaaaagaa cactaaaaaa ttcataacta tattctaatg
    13561 catatccata ttctaacata ctatgtgaac tagtttttac attagtaaaa atagataaaa
    13621 tagctaaaac taatccataa aaaattccaa ctcctagaga atttgctaat acagtttttg
    13681 aatctctaca tctttttctc ataaaaatta ggtttgatat taataatacc aaaataggga
    13741 ttataagtaa tatcaacaaa cctatattgg catttataaa cccagaaccc atcatggtag
    13801 acatagatac actaatttga cccaagttga gagctaggat tatatgtgct ggatttatta
    13861 aataactaat atcattaaat tgaatagtta taatagcttt aaatatcaat gcaataataa
    13921 acaatataaa tatggttgta aatgttgtta aaaatacacc tcttttattt gcatgatatg
    13981 aaagctcttt tattttagat ttcaagtcta gttttgtttc aagtggtctt aatgatgcta
    14041 tttcataaag ttcatttcca caaaatttac agtaattatc cttagtatta ttttctttat
    14101 tgcaactaga acaaattttt tcttctttac ttttaagagt gatatggcta ttttttattt
    14161 ttctagttgg ttcacctgtc tttttggaat aattattatg tgaataattc atattattta
    14221 ttttcttttc acccattttt tccctccaag ttttactatt gtaaattatc aaatataact
    14281 atatcatata aaacttaatt ataccttaat tggatatatt tatctatttt atttgtttat
    14341 tgctgttgtt ttttaacagg tcttgatatt taaatagttt gttaatgcga actttattat
    14401 ttatatttta ttatgcttaa tttaaattta ttctattctt tattttcagt agtaaactat
    14461 ctgtaaatct taattataat aaagtatt
//
LOCUS       DAYAOY010000106        14454 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYAOY010000106 DAYAOY010000000
VERSION     DAYAOY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.106"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(1006..1557)
                     /locus_tag="ACGI6B_003276"
     CDS             complement(1006..1557)
                     /locus_tag="ACGI6B_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9752692.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1748..2710
                     /locus_tag="ACGI6B_003277"
                     /pseudo
     CDS             1748..2710
                     /locus_tag="ACGI6B_003277"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
     gene            3005..4849
                     /locus_tag="ACGI6B_003278"
     CDS             3005..4849
                     /locus_tag="ACGI6B_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFG9752693.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5171..5755
                     /locus_tag="ACGI6B_003279"
     CDS             5171..5755
                     /locus_tag="ACGI6B_003279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9752694.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(6858..7034)
                     /locus_tag="ACGI6B_003280"
     CDS             complement(6858..7034)
                     /locus_tag="ACGI6B_003280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752695.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            7609..8631
                     /locus_tag="ACGI6B_003281"
     CDS             7609..8631
                     /locus_tag="ACGI6B_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752696.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            8645..9532
                     /locus_tag="ACGI6B_003282"
     CDS             8645..9532
                     /locus_tag="ACGI6B_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752697.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            9538..10311
                     /locus_tag="ACGI6B_003283"
     CDS             9538..10311
                     /locus_tag="ACGI6B_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752698.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            10423..11148
                     /locus_tag="ACGI6B_003284"
     CDS             10423..11148
                     /locus_tag="ACGI6B_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752699.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            11152..12555
                     /locus_tag="ACGI6B_003285"
     CDS             11152..12555
                     /locus_tag="ACGI6B_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9752700.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            12566..13390
                     /locus_tag="ACGI6B_003286"
     CDS             12566..13390
                     /locus_tag="ACGI6B_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFG9752701.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(13715..14335)
                     /locus_tag="ACGI6B_003287"
     CDS             complement(13715..14335)
                     /locus_tag="ACGI6B_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9752702.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         5681 a         1401 c         2220 g         5152 t
ORIGIN      
        1 atagattgaa atttatgaat attcatacta taatttaaat ataaggagat gccctttgaa
       61 aataaaaatt aaaaaatatt taatgctact acaataggaa ctagaactac acttaataaa
      121 tatacagaaa ttagatttgg ctcaatataa aatacaaata tagaatttag gtgtttttta
      181 tgaaaaaatt tttatatgct ttatatagtt tcattgttat tatagctaat tttagattaa
      241 aagaaaaaaa ttataacttt attttattag caatcttttt tatattgctg ttaattcaaa
      301 atggagtttt aaaaacaggt tatcaaaata gagctacatt ttatcaagat aattatttta
      361 atagagagga taaaaatgat aagataaaat ttatgactat aaagattttt atatttttgt
      421 cattaccatt atgttttaat atactattca attatttaac ctcttaaatt atatttagat
      481 ataggagtct tatgataaaa gtaagcttat gtgttatttg tagtaaatat aaagtgcata
      541 tagtgataaa acaaaagttt catataaatg aagaaaaaaa tataaaataa tttatttaac
      601 tacttaaaaa taaactaaaa gggataataa aatagctttt aggttatggt aataaaggta
      661 tttaaaaaga gtgcataagc actccaattg tgagaaatac atacacaaat cattattaca
      721 acaatgaaaa atgtacatat aagagacgtt ttgtatttgt tttaagtgat aatattttaa
      781 tactaactaa ataattatca caatagtaaa aaagaaaaag gagtattttg tactccattt
      841 gtcaaaaata taaaactttt atataagctt attataacat aattgataaa agtgtaaaga
      901 tatataatga atttataaag aaaaataaaa taaaaaagat aaatcctaat caaaggattt
      961 atctttttgt atagaaaata attattctat tattaaatgg catggctaaa ctggataata
     1021 atttattatt actgctttca atgttttttc tccttgatta tggtatgtat gaattgattc
     1081 agctgaaaaa caaatagagt cattttctct aagttgataa atagagtcat ttacttctaa
     1141 ttttaattga ccttctataa tcataatata ttcctgtgat ttttcagaat gaccaacaga
     1201 tgtataacta tgaccttctt ctaattccat ttgaaataat tcgaagtttc tagttggtgt
     1261 attgggataa taacaataaa gacgataatc tttattttct gaaacttgta catctatatc
     1321 agtttttgat acaataaaag tttcattttg aggttgttcc agtattgcag tatatggaac
     1381 atttagtcca cttgcaattt tccaaattgt gtttactgtt gggtttgagt ctcccttttc
     1441 aatttgcgaa agcattactt tacttacgcc agataattca gcaagttgac ctagacttaa
     1501 gttacgttca ttacgtaatc tatttaaatt tttagcaata acagtattaa tactcatagt
     1561 agctccttaa aatgtaaatt attgacaaat ataatttacg aatgtataat ttatttaaca
     1621 ttatttataa tttacaaatg tataatataa tgaacgttaa atacactgta catccgtaat
     1681 ataaattata ctgcataaag agttgttttt caacggatga tagttgagag attaaggggg
     1741 aattgtaatg cgtgtactta ataatgttac agaggctata ggcaatactc atatgttaca
     1801 tttatccaaa atagcagatg aatttggagt tcaaggtaat atttatgcaa agatggaata
     1861 cttaaatccg ggatttagta aaaaggaccg tgtagcatta caaatgatta ccgaagcaga
     1921 aaatgcaggt ttattagttc caggtcaagc agttgtggaa ttaacaagtg gtaatactgg
     1981 aacaggacta gcaatagctt gtgcatgtaa aggatataaa tttatagcgt gtatgtcaaa
     2041 aggaaattct atggaaagag caagaatgat gaaagcttta ggtgctgaag tagttcttgt
     2101 agaccaaatg cctaattcag ttcatggaca ggtatctggt gaagatttgg cacttgtaga
     2161 aattgaggct caaaaaatag caaaagaaag aaatgcattt agagcagacc aatttaattt
     2221 agaagcttgt aatcatgcac atgaaaagta tacagcagaa gaaatgtggg aataattgga
     2281 tggaaatatt gatgtatttg tagattttct tggaagtggt agtacttttg caggatgttc
     2341 aaaaacactt aaaaaacata attcttctat caaatgttat gttgttgaac ctgaaacagc
     2401 agcaatttat tcaggtaaag aaataactga ccaagggcat aaaatacaag gtggaggata
     2461 ttctatggat ttgccattag tagacaagaa acttattgat ggttatattc aagttacaga
     2521 cgaggaggca actgatgtag caagaaaatt agctaaatta gaaggtgtat ttgcaggatt
     2581 ttcttcaggg gcaaatgtat gtgcagcaat taagttgtta aagagcagtg aaaaaggaaa
     2641 aaatatagtt ttaactctta atgatagtgg tttaaaatat ttaagtactg atttatatga
     2701 agaattgtaa taatagcaca atagttatgc aatgtttttt aaattaagta tatagattta
     2761 aattataaaa agtatctcaa tgtaattttt gaaagttata aaggagatac ttttttattt
     2821 tgtaaatatg gagtatccta cttataagta ttaagctata tagatattga tgattagcta
     2881 tgcaataaaa aagagtatgt gtttttgcat ttatacatca taacttggaa taatacatat
     2941 taatgttcta aatgtattta aaacatatga taaaattaga acaaatagat acaaggagat
     3001 aaaaatgcaa aagagaattt taagaaattt tatatttacg ggtaaaaata ttttaagtaa
     3061 acttgttttt ataataaaat ataattttag tacattaatt ttatttgaat tatttcataa
     3121 aggattagcc ttatttttta tatggccatc tattagatat atatttgata ttttaataaa
     3181 aagtttacat atagtctacc taaatatgga taattttata aaagttttga ccaatcccat
     3241 gtcaatagtt ctgataatac tatcagttat aatactagct ttttatgcat tttttgaatt
     3301 tacctctgta attatttgtt ttaataaatc gataaaatat gaaaaaatag ggttatttga
     3361 actctttaaa atatcattta agaactctat aaaattgtta tatcctaaaa atatactatt
     3421 atttatattt gtgatattaa taattccatt agtaaataca gttttaattt cagggtttat
     3481 aggaaaaata caaataccag agtacatact agattatata aagtctgata taattttaaa
     3541 tataatctat atgatttttc ttttaataat atatatttcg gttattagat ggatttttag
     3601 cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag aaagtgttaa
     3661 tcttataaag ggaaagatta taagaacaat tatttattca ttaatattat ctttagtagt
     3721 agctattatt ggatatgcga tttatcatag tggaattata tttataggca tatggactaa
     3781 atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta ttgcatttga
     3841 agaatatttt agattgattt tatcaatatt catttttata ttggacattg gatttatctc
     3901 agcaacttat tatcaatata aaggagtaaa tatttctaaa aataatatta aaaaagagaa
     3961 aaaatctata tcaaaaattg tttttaattt agtattaata ttattgattt cttatataga
     4021 gtcagttgca ttttcttcac atataaatgg tatgtttaat acgtcatttt cattttatac
     4081 aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt tggcagcaat
     4141 agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag aaactaaaga
     4201 tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag tagataaaaa
     4261 tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct tttattatta
     4321 tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac tcaaagaaat
     4381 gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta atggacatga
     4441 aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt ttgaaaatca
     4501 atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac tggatgagaa
     4561 tttattgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt taaattatgt
     4621 tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa agattcataa
     4681 aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg agaaaatgct
     4741 tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga gtgcaattta
     4801 ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa tattctataa
     4861 ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt taacataaat
     4921 aaggttgtta aattaaggtt attcttataa aaattgtgct tatcagctat aaacaaggaa
     4981 taaaagtgaa aaaattgctc tgagattatt acagttaaaa gagttttaag atgacagaga
     5041 gatagattgt caactgtata tttaataaat cttgttattt attaaatttt atataagtaa
     5101 aatatataaa ttaataaatc ttatgctaga atatatatga aaaacaatat aaatttggag
     5161 gattaaaaac atgacagagc gagaaaaaat gttggcagga gaactttacg attgtggtga
     5221 tattgagttg ttaactcaat ggcataaagc aaaaggccta gtaagagatt ataaccaaac
     5281 agattcctcg gatttggaaa aaaaagaaaa aatattaaat gagttacttg gtggtaaggg
     5341 taaaaactta tggattactg caccgtatta tgtagattat ggaaataata tttattttgg
     5401 aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa ttatcattgg
     5461 ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc caactaatgc
     5521 tggtgataga tttggtaatg ccaaagaaga tggttcattt gaattttgta aaactcaaac
     5581 agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta tcatgccagg
     5641 tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa aagatattcc
     5701 aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaata aataaagtta
     5761 aattttcttg caaaatctca aaaaacacct ttcttacttt atacataggg tattttctaa
     5821 tactttcaca tccatattca tttgttaaat ttttgtggaa tgtagttttt atcacttcta
     5881 tcttacattt ccaccatttt tttataaaag tcattccttt atagatttat ttataatagt
     5941 atatattcta tttgatgttt gaataagcac ttatttttaa gtaaaatact aacttttagg
     6001 ttagtatttt gctatagtat gatgattaag taagaataaa atactataat acataaagct
     6061 taataaatat aatttttatt ttacttagat attttctttt gattagcaat caaaagtagt
     6121 tataattttt ttaattaata agagtaacat ttattttatg aaagaggatt atattaatat
     6181 atatttaaat aggaaaaatt gtaaaaagca taaaaataat ataattaatt aatgagataa
     6241 aagtggataa aaatattggg agtttaattt ttgtgaaatt aaaaatatta aagttaatct
     6301 acaaatatag aatatattct aagacgaatt tttataatta ctaaataaaa taaattaatc
     6361 aatatagttt tttaatataa acaaaaaata aaaaacttat ataaataaaa tgcttgtttt
     6421 tcttgtatta tttatataaa tattgaaatt tgtctagtta tacgttttta ttgaccagtt
     6481 atgcagttga tattgaatat tctaaatatt ggtgttattt tataggtggt ttaaacatat
     6541 tagggggatt ttatatatga tttgtatgat ttatagagaa tgtataaaat agtaaaggtt
     6601 tagagccatg tttagtggat tgcagaatat gcatatagaa tgattcttgt aaattaagtt
     6661 agacatagga aatatataat ttgtattgga tgattaattt tatggtgtat gcttttaact
     6721 tctataatgg gaaagtaaag atgtactccc aatacatctt tactttagca gaagttatag
     6781 ttacttataa agtacaaaaa attatatatt tattagagta gataaatcta aaaaggtttt
     6841 tctagaaaaa tttagtttta attgtatcta cttactaata ataagacttg atgtaagcag
     6901 gtttcaacta ccgtttctcc atattctgat ataaaatcat ctaactgtgt atctaaaaaa
     6961 ttacaataat catatgaact actgatgtaa gcagttagta tatgtgtaca tttgtctatt
     7021 tctccaataa acatattatc catcccccta aaaaatttag taaaaataaa aagtcgcatt
     7081 ctattagtca attaacataa tttataaaat tttttagtac tagtttctat aacttaactc
     7141 cttaaggtta aatattccca gttttaccaa tttagtttta ttctacaagt atataatact
     7201 agaagatgct ccattattca acatgcttgt ttgagaatag aaaatattct aaaattataa
     7261 ttgtttttaa aaataagatt caagataaat aaagttatat gcaactattt aataatcttt
     7321 ccataataag gacagtaggt tctattctta aaaaactcca tctttaaatt taaagaataa
     7381 ctacaatttg aacaacaata aaatttccaa tcaataaaaa tatcaaaaat aataaaaata
     7441 attgaaaatg tataatttcc cagaaaaata ataaatgtaa ctccaataat aaacacacct
     7501 ataaattata aaataattta agctttattt aaggttagta ttatatttat ataagattta
     7561 agttttaata tatatgtgta attaaaatga tttaggagga gaaatatatt ggtaaattgg
     7621 gaaataaaaa aattggctaa atctaagagt atgtttatat ctctagggat attagtgtta
     7681 atactaatga ttagtatctt tattacgcct gttttagaaa cagaaagtta ttatatagat
     7741 aaagaaaaag gacgtataga agatacgaga agtggtatcg acgtaggtaa tgagaaattt
     7801 caaaataaga ttaatgtttt aaagcagttt tcaaatcaaa cagatacagg tgaattttct
     7861 aaaaaaattg aattaatgtc aaagaaaaaa cttgataatc taagtgtgaa tgaatataag
     7921 gatattagtt tttggaaagt atttaactat agagtcacaa attcacttat aaatgtagga
     7981 atgttaatca ttatatctat aattatatcc aatatttatg tagatgaagt cgttacttcg
     8041 gtaaaagata tcatactatc atcaaaagag aagaaaaaag cattaaagtc taaaatattt
     8101 gttgctttat tagttccatt agtaatttat tctgtttatt taatgggaat atttattata
     8161 acttgtatgc aacaaggagt acctgttaat ggagacttag aatcttttag aatagtagac
     8221 aatgtagttg cattgaaaac taatttaagt ataacaaagt atgtaatatt aaatataggc
     8281 atagagcttt tgatgtttga aggatgggcc atgatagcca tatttgggtc ttttataagt
     8341 aagtcttcaa tatcttcaat aagttttttt atatttatta tatctataac taagataatg
     8401 tcggtaatcc atatattacc taaaaaatta ctttcagttg tatcaaatgt aaattattat
     8461 gatttgatat ttggttttaa taaaataata ggaaattatc taggtgatat tatgatattt
     8521 aaagtgaata tagacattgt caatatagca ataggacttt tgatggcaat attgataggt
     8581 gctatgtcat tatgtttttt agtaataaaa tcagattata taagtagata aaaggggagt
     8641 taagatgaat aaattagaaa tagaaaatct ttcaaagata tatggtaaaa aagtagcaaa
     8701 cgacaagatt acagtaactc tggaaaatgg agtttatggt cttctaggac caaatggagc
     8761 aggaaaatct acattaatga aacaaataac aacgttgata aaacctgatg aaggtcagat
     8821 actttataat ggtgaggata tatttaaaat ggatgctgat tatagaaata ttttaggata
     8881 tttgccacaa gaatttggag tttataaaaa ttttacagca aaagaatttt tacagtatat
     8941 tggagcttta aaaggtttaa ggggaaaata tttaaatagt aagataggag aattattaga
     9001 tttagtaggt ctttatgatg tgagaaataa gtctatagga aaattttcag gaggtatgaa
     9061 gaggagagtt gggatagcac aagttttact aaacgaccct aagataatag tccttgatga
     9121 gccaacagca ggacttgacc cacaagaaag agctagattt aggaatttaa tagcaaaaat
     9181 gtctagagat aagataataa tactttctac tcatataatt tccgatatag aatcagttgc
     9241 aaaggaaaca ataatgatta aagatggcaa actcctaatg aaaggaacac acaaagacat
     9301 attaagtcac atggaaaata aagtatataa tgttcaagtt aaaaatgaat gtgaagttga
     9361 tgatatacaa tctaaatata aggttgtaag tattcaaagt gatattgatt cagtagtact
     9421 tagaattgtt tcagaggata ttccaagcga ggataatata caaccagttc aagcaaaatt
     9481 cgaagatgta tacatgtttt attttgattt agaagatgca agggaggtgt agtagaaatg
     9541 atgtatttag ttaaagctga aatgataaaa atattaaaat caaagagtgt ttggataatt
     9601 tggctgtttt taatattttt tggatttttt ctgattagaa agtttgatgt tttagatact
     9661 tatgcagata tattttacaa gatagaaggc tcaatacctc taataggttt gataatgttt
     9721 ataataactt ctggaaatta cattaaagaa tatgattcta atatgacagg tttaataaat
     9781 acaactaaaa agggaaaaaa atcagtagta ttatcaaaac tagtagctaa tggattaatt
     9841 ttatctatta ttaatatttc ttttatattg ttagtaggaa ttagaggttt tagttttttt
     9901 gattttaaaa atttaaatga atcgattcat aacttatggt actttggaaa tagtaattta
     9961 aatcttacag taatacaaat gtatataatt gttatattga ctactatttt tgcttctttt
    10021 atatttgcac aaataggctt aacactttca tctatattta aatcggctgt aattccattt
    10081 atactaggtg gattgataat ggcaattcca tactttagtg taggttttat accagataaa
    10141 gctataaaat ttatgtcagt gactccaaat tggattatga tgagccaaca aatggttaag
    10201 tataatgtac caagtatttt gatagttttt tctatagtaa tctcaattat attaatgata
    10261 gtgttaacta aaataacata tgaaaatttt acatcaagca aaaggtttta actaaaaata
    10321 atagtaattt tatttatatt ttagtttaat taaaaataag ataaatgaat tttagataaa
    10381 atatatgcta atatatatgt aaattattac aggaggttgt ttatggaaaa tcgtgtttta
    10441 ataatagatg atgaagtaga aatattgaag cttttagaaa ctgtcctaaa aaaagagggc
    10501 cttaataata tatatactgc aaaaactaaa aaagaaggat tagaattatt taaaagtatt
    10561 aatcctgatt tgattgtatt ggatattatg ttaccagatg gcgaaggtta tgatatttgt
    10621 aaagaaataa gaaaaacctc caattctcca ataatatttt tatcagctaa aaccgaagaa
    10681 ttagataagt tacttggact agcgattgga ggagatgatt atgttacaaa accttttagc
    10741 cctaaggaag tggcttttag agttaaagcg catttgagaa gattaagtta ttttagtgat
    10801 gctcaaaacg aaagtaaaaa tctaaataat aatgaagaaa aaattatatc atttggccca
    10861 tatatattaa atgaaagtag ggcagaactt ataaaagatg gaaaatctat aggactgtct
    10921 gcaaaggaac taaaaatcct atctttattt gcacataatc aaaatcaaat tataagtaaa
    10981 gaaaaactat gggataaagt gtggggagaa gattatgttg gatttgacaa tactataatg
    11041 gttcatataa gaaaaattag ggaaaaatta gaggataatc cttcaaagcc tgaatatatt
    11101 ctgacaataa aaggtcttgg atataaatta gcagtaaagg aagattagaa tatgaagtgg
    11161 aagattacta gaaattttat atttacaatt gtgtttgtag ctatatcagt agtaattata
    11221 aatatcatat ctatattgta tgtgatttca actaatagtt tttttaaagt tgtagattct
    11281 ggaaataatc ctgaagaatt tgcacgttct tttgaaaaag atttatatga aaaagatgga
    11341 gaatttaaac tatctaagat tggagctgaa aaattagaaa aatcaaattc ctggatacag
    11401 gttttaaatg atttaggaga ggaagtttat ggagtaaatg tgccaaagga tactcccaaa
    11461 aaatatacac cctttcaaat ggtgaataat tacaagtata tagaaacaaa gtatgttaat
    11521 tttgtattag aaaaatattt aaacaaaaaa catctaaaca taatagtagg tatacctagt
    11581 agagatatat caagaataat tttaacctat tctcaaaata acattaaaaa aactttaaat
    11641 aaagttatca ttataacact agtaattgat agcgtggtag ctttaggtgt aggatattta
    11701 tttagtagaa aacttacaaa accaatttca agcgtactat ggagtattga gactatggct
    11761 aatggaaatt atagtttata tcttaaggac agaggtatat atgaagaagt ttttaaaaat
    11821 ataaatatgt tagctgatac tttaagagta aatgaagttg agagaaaaga aaatgaagag
    11881 ttgagagagg aatggttggc aaatataaca catgatataa agacacctct agcatctata
    11941 caagggtatg cagagataat taatgacaaa gattatgagt ttgaagaaga tgaaatacaa
    12001 gagtatacag aaattatata taataagtct aagtatataa aagatttagt tgatgattta
    12061 aatttatcta ctcgattaaa aaataatact atagttctgg acaagaaaaa aataaattta
    12121 gtcagtttag tcagagatat aataattgat attttaaatg ataatagata taaaaatagg
    12181 aatattgaat ttgaaagtaa tgaagattta attgaagtat atgtagattc aatcttattc
    12241 agaagggcta taaccaatct tatatttaat tcaatagttc ataatagcga aggaactttg
    12301 ataagtgtag aaattgtaaa aaaagataat atagaaatta taataaaaga tgatggaatt
    12361 ggaataagca aaagtgattt gaaacatatt tttaaaaaat attatagagg tactaataca
    12421 ggtgagatgc ataaaggttc aggtcttggt atggctattt ctaaagaaat aatagagatt
    12481 cataaaggta aaatttatgt aagtagtgaa attggaatag gaacaaaaat tattatagag
    12541 ataaaacaaa attaggagat atgatatgga tttttttaaa aaaggtataa gtgggtttac
    12601 tattaagatt ttagcattaa tatttatgac atttgaccat atagcagcat ttatgccaca
    12661 aacaatgcaa ataccaattt ggtttcattg ggtaggcaga atatcagcac cactgtttat
    12721 attcatggct gttgaaggat tttatcacac tagtaacaga aaaaaatata taagtagatt
    12781 gtatatttgg tctgttatta tggctatagg aaatcaaata attaataatg tattttcaca
    12841 tccagaagga gctataatta taaataacac tttttcaaca ttatttttaa tagcgattta
    12901 tttacaagca atagagttca taaagaaatt tagaaaagag aaagaaatta aatattttat
    12961 tataggatta ttgatgataa tcattcctat aattttagga atatttacag tagctttact
    13021 ttttaaagtc actaacagag ttattgcttt atttatgatt ttggtaccag taccattttt
    13081 agtagaaggt ggaccaattt ggattatact tgggattatt ttttatttgt gtagaggtaa
    13141 aaagttcagt ttgagtattt gttatgtttt aatgtgtata tttatattta caacaatgtc
    13201 aaatggagat tatagtttaa aaaattctat tttacaaaat taccaatgga tgatgattgc
    13261 ttctctacct ttaatgttac tttacaatgg agaaaaaggg aagagtatga agtatttatt
    13321 ttatttatac tatccaatcc atgtatatat attgtatatc ttaggaatat acttaataaa
    13381 tggtttttga ttgtaagtga tatttttaat acaaaatgaa cagtatgagg attgttttag
    13441 gtctattaaa attgttattt agatgagatt tgtagaatat ttaaatttat gagcagaaat
    13501 tcagttattg taatttcaat aacattagaa tataatatta taatttattt agaaatatat
    13561 gatgttatac atggtttggt tattaatgta ataataaaac agaggtttga tataaaattc
    13621 aatacctctg ttttagtgct ttttatccta catttatgct tattttattt ataaattagt
    13681 gttttgtttt aattaatatt agtacatttt ttatttattt tataaaatta gtcataattg
    13741 tttcttcata ttgtggttct ttaattccag cttttttacc tgctgatata cacttaagaa
    13801 gccatgccat gttttctcct agagttcgca tagtctgtaa tccttcttca tccttcataa
    13861 catcctctgg agtaaagcca tgtacctggt tccaatactg tgatgataca ataggcatat
    13921 tgctgattga aaaatattta tttagttggt caaatgttgc acttgcacca ccacgtctac
    13981 atgacacaac tgaagcacct aattttgtag ccatgcgact cccagctatg aaaaatagac
    14041 ggtcaagaaa agaagttaat tgaccagttg cactagaaaa gtaaacagga gaaccaataa
    14101 taataccatc aatattatcc agatcttcaa tcaattcatt aactttatca tttcgaatac
    14161 attttccagt ttcaaaacag gatgcgcaac taatacatcc tgggattgtt tctttaccta
    14221 aatacagtat ttctgtttta atatcgtgtt tactcaatac tttagtaatc tcatttagtg
    14281 ctgtataagt acatccatat tggtttgggc taccgtttat caataaaact ttcattataa
    14341 atttcctcca atgaatttta tttaatagat tttctaagag acattatttt ttataaaata
    14401 ggattttcat gaatattatc aatttatata gaaatgtcta atcttagaat ccta
//
LOCUS       DAYAOY010000107        14267 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAYAOY010000107 DAYAOY010000000
VERSION     DAYAOY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.107"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            166..1188
                     /gene="splB"
                     /locus_tag="ACGI6B_003288"
     CDS             166..1188
                     /gene="splB"
                     /locus_tag="ACGI6B_003288"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HFG9752703.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            1370..2281
                     /locus_tag="ACGI6B_003289"
     CDS             1370..2281
                     /locus_tag="ACGI6B_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9752704.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            2296..2712
                     /locus_tag="ACGI6B_003290"
     CDS             2296..2712
                     /locus_tag="ACGI6B_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFG9752705.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(2812..3204)
                     /locus_tag="ACGI6B_003291"
     CDS             complement(2812..3204)
                     /locus_tag="ACGI6B_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9752706.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            3345..3740
                     /locus_tag="ACGI6B_003292"
     CDS             3345..3740
                     /locus_tag="ACGI6B_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9752707.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            4143..4349
                     /locus_tag="ACGI6B_003293"
     CDS             4143..4349
                     /locus_tag="ACGI6B_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752708.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(4661..5404)
                     /locus_tag="ACGI6B_003294"
     CDS             complement(4661..5404)
                     /locus_tag="ACGI6B_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFG9752709.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            5570..8137
                     /locus_tag="ACGI6B_003295"
     CDS             5570..8137
                     /locus_tag="ACGI6B_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9752710.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            8358..8771
                     /locus_tag="ACGI6B_003296"
     CDS             8358..8771
                     /locus_tag="ACGI6B_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9752711.1"
                     /translation="MKLVIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNI
                     EDLNKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLE
                     ALINREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            8795..9292
                     /locus_tag="ACGI6B_003297"
     CDS             8795..9292
                     /locus_tag="ACGI6B_003297"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9752712.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            9295..9696
                     /locus_tag="ACGI6B_003298"
     CDS             9295..9696
                     /locus_tag="ACGI6B_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9752713.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            9714..10202
                     /locus_tag="ACGI6B_003299"
     CDS             9714..10202
                     /locus_tag="ACGI6B_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9752714.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            10229..10984
                     /locus_tag="ACGI6B_003300"
     CDS             10229..10984
                     /locus_tag="ACGI6B_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9752715.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            10984..11847
                     /locus_tag="ACGI6B_003301"
     CDS             10984..11847
                     /locus_tag="ACGI6B_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9752716.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            11894..12373
                     /locus_tag="ACGI6B_003302"
     CDS             11894..12373
                     /locus_tag="ACGI6B_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HFG9752717.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            12438..13745
                     /locus_tag="ACGI6B_003303"
     CDS             12438..13745
                     /locus_tag="ACGI6B_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9752718.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            14073..14195
                     /locus_tag="ACGI6B_003304"
     CDS             14073..14195
                     /locus_tag="ACGI6B_003304"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFG9752719.1"
                     /translation="MLSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDENGE"
BASE COUNT         5582 a         1435 c         2598 g         4652 t
ORIGIN      
        1 aattaatagt aaaaaaattg tgtatagaag ttatatatat aataaatttg tcttaagctt
       61 ttattttact ttctaataaa taaaggagtt taaaaaaggg aagattaatt aagtaatatt
      121 ttataaaatt cttcgattta aaaactttaa gtagaggtga aataaatgtt taatcccaaa
      181 agaataatat tcgaaaaagg aactttagac actgatgtgg gtaaaaaaat atacaataaa
      241 tttagagata ataaagatgt agagataatt aatatatcat caaataggat taaagaacat
      301 attccaggtg aaaatctata tgaacaatat agaagtggga agcagactat ggtagttggg
      361 attagaaaaa gtctgaagtt tcaaacatgt aaaccttctg ctaattatca actaccactt
      421 gtcagtggat gtatgggaag atgtgagtat tgttatttga acacacaatt aggtgataaa
      481 ccatttataa gggtgtttgt aaatgtagat gaaatacttg aacagacaaa aaaatatatt
      541 gaagatagaa agccacaggt aaccatattt gaaggggcag ctacatcaga cccagttcca
      601 gtagagccat atactaattc tttgaagaaa acaatagagt tttttggaag agaggaaaaa
      661 ggaagattta gatttgttac taaatacagt gatgtggata gcttattgga tgctgaacat
      721 aataatcata cagaaatacg atttagtatc aatactccta gaattataaa tgaacatgag
      781 cattatacag cctctgcaaa taaaagaata gagtcagctg taaaacttgc tagggctggt
      841 tatcagattg gtttcataat agcacctgtg tttttatatg aaggttggaa agaagagtat
      901 aaaaatctga ttataagtat aaaagaagct ttaccaaagg attttgataa aaaaattata
      961 tttgaagtta tatctcatag atatacaact aaagcaaaaa atagaatact agagatttat
     1021 ccagatacac tattgccaat ggaaaatgaa gatagaaagt ttaaatacgg tcaatttgga
     1081 tatggtaagt acgtgtatga taaagaacaa ttagatgata tgaaagagtt ctttcttaag
     1141 gaaataaatg gagtatttaa aaatagttta ataaaatata taatatagca aatcttacct
     1201 aaagatttgc tatattttat ttttatatat tttatgttta taataaagca atgagattta
     1261 gcattatcat ataagaaaat taaaaagggc actatctatc aagaacattt tttctagttt
     1321 tgatactata aaggataaga gtaacaaaaa tatcaggagg agttatagta tgcatgcatg
     1381 ggagtctatt caaattacat tggatttaat agaagcaaat ttatcagaag aaatatcaat
     1441 taatgaatta gcaaataaag caaatttatc accattttac tatcaaagac tatttaaaag
     1501 attggtaaat aagactgtaa tggagtatat aaagcttcgt cgtcttgcaa gagcttctga
     1561 atacttagca gaacataaga atcgtatact tgatgtagct ttaaattttg gatttgctag
     1621 tcatgagaca tttacacgtt cctttaaaaa agcatatgga ttaactccag aaaaatatag
     1681 agcaaatcca gtaaaactca atcattttat taagccagaa ttaattttaa attatgctat
     1741 ggttgatgaa ggtgttccac tgattgcaga tgatatagtg attgaagtca atcgtaagac
     1801 attgagtaat ccaaggacat ttgttggaat agaaatagaa gtacctattt gccagttggt
     1861 gggcggtgaa acaactggaa ttgcaatagt agaggaatta tggatgaaac ttggaagtca
     1921 aagacataat ataccaaatc aaattccagg tggaaatgaa tttgctgctc tttatatggg
     1981 aaatgcaaaa gaaggaaatt gtatgtatat ggcaggtgta gaggtagagt caggtacatc
     2041 agtagaagga tatagtactt ttgagttgcc agctaaagaa tatttagtat gtggatttga
     2101 ggcagaaaac tttaatgagt tagttaattc agctgttttt aaagcagata aatttatgga
     2161 aagatggatg aagaaacata atttaacaac tactgatttt gcaattgaga tgtattaccc
     2221 cactacacct gaagcagcat atctggaaca ttggattgtt ccggttccta ttgaacaata
     2281 gaaaggatga tgaatatgaa ttggcaagat gcgttttttc aagatactaa acctacattt
     2341 gagcaaatta atgaatttat taataatcca ttatgggaca agttaaataa aactttggtt
     2401 tctacttata atgtagaacc taagcttgaa tacagtaaat gttcaatgca aagaggttgg
     2461 aatgtaaaat ataaaaaaag aggtaaatca ctatgtactt tatatccaca agaaggttat
     2521 tttaaagctt tggttattgt aagtgaaagc aatagagtag aggttgattt atttattaat
     2581 acttgttgtg attatatcaa aaaaatatat aatgaggtaa acttttttaa tggaagtaaa
     2641 tggctaatga ttcaagtaga tagtttattg gtattaaacg atatgcttga actaattaaa
     2701 tttagagctt aacatataga acaggatgaa tatataagtc atcctgtttt tatattcaat
     2761 cagataaatt aatatatagt ctataatatg aataatttag taaatttaaa tttactttat
     2821 ctttctacat tctttgcaac taagtccaca tgtgtttttt tcttgcatat tagtagcacc
     2881 ccaattagca aattgttgta aaataggcac aatactttta cctttacttg ttaatgtata
     2941 ttcaactttt ggaggaattt cattatattg tttacgatga acaataccag tagactctaa
     3001 atcttttaat gattgagcta gcatagtatt tgtaatgcca tccaattttc tttttatgct
     3061 actatatcta attggatttt caactgctaa catacatata ataggtaatt tccacttacc
     3121 accgacaatg tctaatgcat aacggattgg acaggttaat tcttcaacgt ttttagttat
     3181 atttttttca gtatttatac tcattttaat tactccttag ttatagtagt atctttttta
     3241 tactttatca gaaaaaactg catacttgtt ttttctgtat tttatattaa aattatatca
     3301 aaggataatc cttaatacaa gtatttaaaa ggggtgtaag aattatgaaa gcattagaat
     3361 ttttgaacaa ggcaggagtt tattatttag caacagttga tgagaacaat caagcacatg
     3421 tgcgtccttt aggatttgtt atggaataca atggtaaact tacattttgc acatctaatc
     3481 aaaaggatat gtttaagcaa cttattttta atccaaaggt tgaactttgt tgtatagacc
     3541 aaaaccttaa tactctacgt attttaggtg aagcagtatt tgttacatca aaagaaacac
     3601 aagctaaggc acttgaaata atgccaagtt taggtcagat gtactctgta ggagatggta
     3661 agtttgaaat attctctatt gataaggcaa aagttagttg ttgttcaatg agtggtaaaa
     3721 agattgatgt agagctttaa ttataattga atcgacatcc atagcgaaaa ttaagaaaac
     3781 gttattactg aaatattgat agatttaatt taaatgcaat aaaaagattt ttaactaatg
     3841 aatatgtaaa aatttttagt tattagatgt gtatttcaat aatgatatac tattttgaat
     3901 aattacatac tagaaataag ataattaaaa tttactttat aatgggatag atttctttta
     3961 aaaaatattt taactataat tgatttttat ttaatctgaa aaaaatatta tgactataaa
     4021 aaattgctac ttattatttg gatattttta tatataaata ataagtagca tgactttata
     4081 cattaataat gatagaatat aaattaatag ataagatatg attttaaaag gagtgatata
     4141 agatgagtaa atatagttct ttatgggaat atgttaagaa aaacaatagt caatcgttta
     4201 aattaacttt tgaagaaata aaagatattg ctgggataga gatagaccat tcgtttttga
     4261 aatataaaaa ggaattaaat gaatatggat atcaagtagg aaaaatctct ttaaaagaga
     4321 agactgttat tttcgataaa attggttaag acataaataa ttacatataa taaaataatt
     4381 tgtattgatg attattacaa ataaaaataa agtaaacctt atataagatt tactttattt
     4441 tagatgattc ttttttttag ctttatacta taaggtatat aaaactaagc ttattaaaaa
     4501 tgttataatt tctattgcta tcatagacca tatcaatcca tcaagttcat aaaagtaatt
     4561 aagctttatt aaatagcttc tttattataa tttctttatt tcatataata taaatagtat
     4621 taaaattttt atgggagtta aagtaagcat gtattattac ttagatattt gtataatcaa
     4681 catctagtaa tttacctttt tgatgaaaat gattaataag ttttttagga taattcatta
     4741 ttaattcatc tggaaaatta atttccttta agatattttc agaatactca tagttaccta
     4801 tgtcaaaaca aatgtgagca tctgaagtta gtatgatttt agcaccatat tttttgcaaa
     4861 gtgtagcaat atacttacag ttatccgagc taccatttct tgacacgcct ttaatagaac
     4921 aattatttat ttctattaag atattctttt ctatagctaa tttaatgatt aattcatagt
     4981 cgattgggta gtttgggttt ccaaggtgtc ctaatatttc aatattatca tgcttattta
     5041 ttgcatttat taaagcttta gtattattct ctaatgtatt tggagaaaac acagcttcgt
     5101 gaaatgacaa gataagataa tctaaccgag gaattacaaa aggctctagg tctatgtttc
     5161 ctttaatatc tagtatattt gcttcacagc ctcttaaaat tataatgtta tttatgattc
     5221 ttgggatatt ttgaatgttg tgaaagtacc atctgtgggg agaaccaggc atcttaggac
     5281 catgttcaga tgtacctagt atcttaattc cattttcttt acaataatta atattttcta
     5341 gtaaagtgct atagccatga ccactgacta tagagtgggt gtgtaaatct gataaaaaat
     5401 tcatataaat tcctcctcta taaaagatga gggtaacgta atttatatta taaaacgaat
     5461 aggaaagaaa atcaatagtt acataattag acaaaaatat taaaatattg tagaattaag
     5521 cttaataata aatgtttaac agtattactg cttatagggg aggttaacta tgacacaaaa
     5581 gaagctatca aggaaagaaa aagttttaat ttgcttgaaa aggttaacag aagaacactt
     5641 gaactttaat aatggcatta gatttggatt tgatgcagaa tatatagcta ctgaagtagg
     5701 tataaataga agtaatgcta gtaaggaatt aaatcaatta ataaaagaaa gtatcattat
     5761 aaagataaag ggtaagcctg ttcagtactt atataaagaa agtattgaga gtattttagg
     5821 aaagaaacta aataaaggga tatttgataa tattaactct ataaacgaat atagtgaaaa
     5881 tgattctatc aaagatgagt atattttaga gagtataaaa aaagagaaca atatttttaa
     5941 tttagtagga tgtgatggaa gtttaagatc ccaaatagaa caggctaaag cagcagtaat
     6001 atatccacct aaggggttga gtacattaat aataggacct acaggagttg gtaaatctat
     6061 atttgcagaa tatatgtata agtatagttt aactataaaa gaagatagtg ataatgcacc
     6121 atttatcata tttaactgtg ctgattattc tgataatcag caattattat tagcgcagct
     6181 ttttggatat gtaaaaggag catttactgg agctgataaa gataagtatg gtattgtaca
     6241 tgaagctaat aatggagtat tgtttttaga tgaagttcat agattatctg cagagggaca
     6301 agaaatgcta tttctgctta tggataaagg gatatataga aggttaggag aggctaataa
     6361 ggtacacgag tgtagagtta tgataattgc agccactaca gaagacccag aaacagcaat
     6421 gttggaaacg tttttaagaa gaatacctgt aactataaag attccgtctt tagaggaaag
     6481 aggctttcaa gagagaatga agcttatatg ttattttttt aaagaagaga gtgttaggat
     6541 tggtgttaag ttaaaggttt caaaagaagt tattaaggca tttattttat ataagtgtaa
     6601 gggaaacata ggccaattaa aaagcgatat acaattaatt tgtgcaagag cttttttaga
     6661 ttatatgacc tataaaaggg aatgtgtata tgtaaggctc tctcttctac caaataatat
     6721 tagagaatca ttatatggaa ataatagaaa agaggagttt gtacaaaatt tcagttttat
     6781 tggaaaagat gatattattt ttttaccaga agaaaaggat tttgaaagtg aaaatttatt
     6841 aatagaaaat aaaaaatatg atttagattt ttatggaatg ataaaagaaa cttggaataa
     6901 acttcaacaa gaaggtatta aagagtcaca aattagaagt gttatagatg gagacataca
     6961 gaagtattcc tataatttaa tgaatacttt tatatataat agtagtaatc aaacagctta
     7021 taacaacatt gttaatgatt caattgcaac tactgtaaaa tatattttac aaaagcatga
     7081 taaatggagc aaaagagaag gattagataa attaataaaa gctatagctt tacatattca
     7141 gaatttaatt gaaagaataa agattggaaa tatagtaaag catcctaata aagatgaaat
     7201 tatgaaggaa agagcatatg aatataacat agcaaaagaa atattatcta acatgtcatt
     7261 aatgtatgga gtagagtttc cagaagatga agcaattttt ttagcaacat ttctttattt
     7321 atcgtatgtg ggattaaatg aggagagtat agcaatatta gtcataatgc atggtgaatc
     7381 tacagcatct agcatggtga atgtagccaa tactttactg gattgtaacc atgcagtggc
     7441 aattaatatg ggattagaag atagagttca ggatatttta gtacaagctg tagaaatagc
     7501 caataaaatt gataaaggaa agggaatttt aattttaact gatatggggt ctattttaac
     7561 atttgctaaa gttattcgtg atgctacagg aaaagaagta aaagcaatag atatggtaag
     7621 tacacctatt gtaatagaag caactagaaa ggctctaact ccagaaatga cattagagaa
     7681 attatatttt aatattatag aatctataaa gaaacattat ggagatatgg aagttacata
     7741 tgctgaaaaa gataatggtg gtagatattt tgatagatta ctaattgata atattagtaa
     7801 aactttgact tttttaaatg gggaaaaggc ttattttata ttaaaagaag taattaatag
     7861 aattagtgaa cactattctt tagtaattca agatgaactt ttagtaaagt ttatatttca
     7921 ttgttcatgt atgattgaaa gagtaataat aaaagagtct ttagtttata aatcatatga
     7981 agaaagaatt agtagaaata aagaattata tttagttata aaagaaagtt ttaagctagt
     8041 agaagagaca ttcgatataa ttatttcaga tatggaatat gcaaatatat tagatatatt
     8101 tgaatcacaa tatgatacag ataaattcaa aagttgatac aaaatttaaa aatagccaaa
     8161 aggctatctt ttttttactt tttttgatac agatttgata caggttgaaa aaatcttttg
     8221 atacatatcg aaataataag tttttaaaaa aaattgaata aaaagttttg attaaatcct
     8281 gtaaaatagc catttgttat tgatacacat aagttggcac ggcaattgct tatataaaaa
     8341 gtgacaagaa aagtgaggtg aaattggtga taggaataat agttatgact catgggagtt
     8401 ttagtgaaga aattataaaa agctgtgaac ttattgcagg accagcagaa agaacagcag
     8461 caattaaact aaataggaat gataatattg aagacttaaa taaaaatttt gtagaaaagt
     8521 taaatgaatt agatgaaggt gacggagttt tagtattagc tgacctatta ggaggttcgc
     8581 caagtaatgt tgcaagttta aaccttaaaa aaggtggcaa gtttcatgca cttactggag
     8641 taaatcttcc aatgctactt gaagctttaa ttaatagaga gggaaaaagt ttagaagagt
     8701 tagcagaagc atgtattgaa gcaggacaaa ctggtataaa caatattaac aaagttctta
     8761 gctcaatgta atagaaaaag gagtttgatt aaaaatgtct ttagtaaaat attgcagatg
     8821 tgatgataga ttaatacatg gtcaagttat atataaatgg gttaagcatc taggtgtaaa
     8881 aaagatagta gtagtagatg atgaaacaac caatgatgtt attgctaaag gtttgataaa
     8941 aatggcagca ccaaagaata ttgatttatc aatattaact gtaagtgaat caaggagata
     9001 tttttataat aatcaagctg atgataacgt ttttgtatta attaaaaatt tagatactgc
     9061 aaatagaatg atagaagaag ggattaatat caaaaaactt attgttggaa gaatacctac
     9121 aggaatagga aaaaagaaaa tatctcaaaa tgtatacatt aataaaaaag agtttctttt
     9181 aatagatgaa tttattaaaa agaatattaa tgtatcaatt caaatggtac ctgatgaaga
     9241 agaagtagaa ttaaaccaaa atattaatcg ctataaagga gagtttattt agcaatgaat
     9301 aaatataagg taatcatact gtgccatgga acttggggaa aagtcttagt agaagaaaca
     9361 aataaaaact ttggattgtc aaatcaatat gaagttttat cactaagttc tgaaaaagat
     9421 gtttctgtat ttatgaaaga ggttgaagaa gtagtagata aagaagatgt gaaaattata
     9481 atatcagatt tatatggagg atctacatca agtgttgcaa tagctgttgc tataagaaga
     9541 ggaatagatg caataaatgg acttagctta cagacaattt taatcgtaga tgaagaatta
     9601 agcaaagaag ataaattaca tacattacca gaaagaatcg tcaaaaaaaa taataacctt
     9661 tgtgtggatt taattaaaga atttaaaaaa atataaatta tgaggtgaat ataatggcaa
     9721 aaataacatt agtaagagta gaccacagat taattcatgg gcaagttata acaaaatggg
     9781 taaaaatcgc tcaagctcaa aaaataataa tagttgatga cttcttaggt caagatgaat
     9841 ttatggctga catatataaa atggcagcac catcaggagt agaagtagtt attttaacag
     9901 cagaagatgc gggacaagca ttccaaaaca acactttagg agataaaaat atatttattc
     9961 tatttaaaaa tgttgatatg gcaaataagg cttataaagc tggacttaaa tatgaaaaga
    10021 ttcaattagg aggtattcca aatgaagcag gaaagaaaat ggtgtttaca gctgtttcat
    10081 taggaaatga ggatgtagaa caacttaatg aacttaatga aaatggggtt gaaatagtcc
    10141 tacaagttat accagaggaa tcatctatga catatgaaaa tgcgttaaag aaatttaaat
    10201 aaaaaatata aattaggagg aattataaat ggaatctgtt ttgatgttgg caattgtaac
    10261 aggattatgg tattggtttg cagcaggtct tgcagggtat acccttttct caacactaaa
    10321 gtctccttta tttataggtt ttagccttgg attactttgg ggtgatgtaa caactggtat
    10381 gattgttggt gcaagtatag aaatggttta tctaggaatg gtcgcagcag ggggaaatat
    10441 tccatcagat aaatgtctag ctgctcttat tgcaatacca gtagcacttc aaactggtgt
    10501 taatgcagaa gttgcagtat ctattgcggt acctttaggt gttataggag tattagttaa
    10561 taacttaaga agaactggta atgctgtatt ggttcataag gcagataagt atgctgaaga
    10621 aggtaataca aaaggcatct ggagatgtgc aacactttat tctttaatat ttgggtttgt
    10681 tttaaggttt ccaatagttt ttgtatgtaa cttttttgga gcagatttag ttcaatcatt
    10741 attagatgtt ataccacaat ggttaatgaa tggattaaca gtaatgggcg gtatattacc
    10801 tgcattaggt tttgcaacaa caatatttac tataggtaaa aataaatttt taccaatgtt
    10861 tattatagga ttttttatgg ttcaatattt tgaaatatca attactgcag ctgcaatatt
    10921 tggtgtttgt atagctcttt taattacctt tatgaaagaa gacaaaaggg taggtgaagt
    10981 ataatgcaag aaataaaaaa taaagaagtt aaaaatggac aaggtccaac tgaagttaat
    11041 aagacaagag tacttacaaa gaaagatgta acaaaaacat atctaagatg gtggtggaca
    11101 gcagaattat ccaactcttt tgaaagaatg caagcattag cagtttgtgc aagtttcact
    11161 ccggctttag aaaagttata taagagaaaa gaagatttag tagatgcgtt aaagagacac
    11221 ttacaatttt ttaatactca agctatatgg ggaggattaa ttcacggtac agttttagct
    11281 atggaagaag agaaagctac tgaaggaaaa atcccaggag aagttatttc aggtgttaag
    11341 aatggtctta tgggaccttt ggctggaatt ggagatactt tagattttgg tacatttcaa
    11401 acaatattct tagctttagg tgcttcattt ggagcagaag gaagtgttat aggtgcattt
    11461 ttcccaataa tgtttagtat attactattt tgcgaaggtt attacttatt tcatttagga
    11521 tattctttag gtagagattc aattaagaaa attctatcag gtggtattgt caataagata
    11581 attgatggtg cttcaattct aggaatgttt atgatgggag cattatctgc aactacagtt
    11641 aagctttcaa ctccgctatc atttgatatt ggtggtaaag caattgttgt tcaagataca
    11701 ttaaatatga ttgctccagg tttattacca cttggtgttg tattcttcgt atattgggga
    11761 atgaaataca agaagtggac aataactaag ttattagtaa ttttagttgt attagcattg
    11821 gtagggtcat tcattggaat attctaattc acagtgatta aaatatagaa tttaatttta
    11881 ggaggagaaa gaaatgtcag aaaaaatgta tacagaaaag gaatatttag aaggaattag
    11941 aggagcaaca ggagaaagag cagtatggtt ctatcttctt atgaaagaag ctgaaaaatt
    12001 aggagctaat ccagatgata tatgtaaaga agcaatatat ggatttggta aaatgagagg
    12061 tcaaaaatat aacgtagcag atactccagg aaaaatggca gaaatgttat ataacagtaa
    12121 aggacaaaaa gtgtttgaaa tggaattagt agaaaataca gatgagaatg gcgttttaaa
    12181 attccatcac tgtccattag atgctgcttg gaaagaatat ggattaacta aagaagaaag
    12241 aaaagaaatt tgtagattag cttgttatgg ggattatgga agagttgact gcgcacaagg
    12301 tgttaaatta gattttgctc aaaaatgtgc tcatgatgat gaagtttgtg aattagtatt
    12361 tacaaaaaaa taatttttaa tgctaaaagc ctgttcttta aggacaggct ttaaaaagat
    12421 agaaagtagg ttgatttatg gatataagca gaagtgtaga taatagttta aatagaacca
    12481 tagaatttct aaaagaattg ataaaaattg attcacaaca aggtgaacct atttcacaat
    12541 gtccttttgg tataggacct aaaaaatctt tagataaaac tttagattac tgtgctagtt
    12601 taggttttag tgttaaaaat attgataatt atattggata tgcagaaatt ggagagggag
    12661 aggaattaat agggattcca atgcatttag atatagttcc accaggagag gggtggagtg
    12721 tagacccatt ttcgggagca gtaattgaca atattattta tggaagaggt gtaatagata
    12781 ataagggtgc tgtatctatg cttatacatg tattgaaaaa catagaggat atgtatccta
    12841 caattaataa aagaataaga ttaatttttg gtacaaatga ggagacaggt atgaaatgta
    12901 tcaaatatta cttagataaa ggtgaagaaa ttccaagtat gggatttact ccagatgcta
    12961 tgtatccagt ggttaatgga gaaaaaggaa gggttcatat aaggatagaa aaagaaatta
    13021 aaattgacaa aagtaaacct tacataatag taaggggagg aactaaagaa aatgttgttc
    13081 catctcattg tactgcaaaa attattaatg gaattatttc agagttaact actaaaggag
    13141 tagctgtaca tgcaagtaat cctgaaaagg gagaaaatgc tatatctaaa atggtaataa
    13201 agatagttga ggataatatg gattttcagc atagagaaga tatcgaatta gtttcaaaat
    13261 acctttgtag tgattattat ggagatgcat tagggattaa tcaatatgat gaggtattca
    13321 aaaacactac cttaaattta ggaatattaa aagtaaatga ggaaaaaatc gtatgtgagt
    13381 tagatataag gtatggtaaa aatatagttt taaacaatat aatagataga ttcaaaaagg
    13441 ttttttgtaa tggttggaaa atagaggtta ttgctcataa agacttacat tatgtggatg
    13501 agagtaattt agtacttaaa aaattattag aagcctatga agaagtaaca gatgaaaatg
    13561 gatatacaat tgctatgggt ggtggcacat atgcaagttg gtttaaagat atggtggcgt
    13621 ttggacctaa gttcttagcg tataaaactg gtggtcatgg agtagatgaa agagtaccaa
    13681 taaatcatat acgaaagaat atggagatat atactttggc tttaataaaa ctattagaat
    13741 tgtaaaagaa gaagagactt tatgtgcaaa tttacagata tcttaaaaag agagataaaa
    13801 cgagttcaac cttatttaaa tgttcaacac catcaaatgg ggctgtctca aaatagagat
    13861 aagcctctta cttattgttg aaatgatgaa aatatatttt acttcataca aaaaagagtg
    13921 ccttatgaag taaaaagtta attttgagac accctcattt gatgatgttg aagagaaaat
    13981 aagtatgaaa ttattaaaaa agatgatttt ataaaagtta aggttgaaaa gaaaagttaa
    14041 ttgaatggaa ttagataaat ggagaaggat gtatactatc taataaaata acaaaagagt
    14101 gttataaagt tggttatatg attagagaaa agtctattga tacttatcca gatagtggat
    14161 ggggatttat gacttgggat gaaaatggag aataataaaa attatagttt atacagttta
    14221 ataagatata tgtacaagca gttaatctaa atgattgact gcttttc
//
LOCUS       DAYAOY010000108        13718 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYAOY010000108 DAYAOY010000000
VERSION     DAYAOY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.108"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            1122..2480
                     /locus_tag="ACGI6B_003305"
     CDS             1122..2480
                     /locus_tag="ACGI6B_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9752720.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2587..3381
                     /locus_tag="ACGI6B_003306"
     CDS             2587..3381
                     /locus_tag="ACGI6B_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9752721.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3374..5680
                     /locus_tag="ACGI6B_003307"
     CDS             3374..5680
                     /locus_tag="ACGI6B_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9752722.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5819..7096
                     /locus_tag="ACGI6B_003308"
     CDS             5819..7096
                     /locus_tag="ACGI6B_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFG9752723.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7279..8001
                     /gene="yqeC"
                     /locus_tag="ACGI6B_003309"
     CDS             7279..8001
                     /gene="yqeC"
                     /locus_tag="ACGI6B_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFG9752724.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            8108..8677
                     /gene="mocA"
                     /locus_tag="ACGI6B_003310"
     CDS             8108..8677
                     /gene="mocA"
                     /locus_tag="ACGI6B_003310"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFG9752725.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8674..9693
                     /locus_tag="ACGI6B_003311"
     CDS             8674..9693
                     /locus_tag="ACGI6B_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HFG9752726.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            10031..11656
                     /locus_tag="ACGI6B_003312"
     CDS             10031..11656
                     /locus_tag="ACGI6B_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9752727.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11855..12331
                     /locus_tag="ACGI6B_003313"
     CDS             11855..12331
                     /locus_tag="ACGI6B_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752728.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12539..13651)
                     /locus_tag="ACGI6B_003314"
     CDS             complement(12539..13651)
                     /locus_tag="ACGI6B_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9752729.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         5383 a         1443 c         2436 g         4456 t
ORIGIN      
        1 aaattttatc aatgagaaca taaaaatttt atactataca aatgtatgca aatattggca
       61 ctaaaattgc tctataaaaa ttataaatag taatatgaat aaaattttta ctataaaaat
      121 aatattacta ttttgcttat agatatatat atctataaat agatagaaac ttgtatattt
      181 tatgttgcag atacaaatat agtaaaggag agtacattgg tacaactgag atacatagct
      241 tattaagttt ttctgtctta tgagaattat attataaaat attcataaat attttgagtt
      301 taaattaaat aattatttta tgcttagatt aattgatatt taattaattt aataacaaaa
      361 acagtgaaag agtaaataat attatttaca gggggtaaag caaagtatgt taaataaatt
      421 gtacttttgg ccccgtacat agagaaatct atgtaggaca caactttaaa accagtaaat
      481 cctaaagcct tgcaactaca ataaaagata gaaagaaact tttatgaatg tggtgaaaac
      541 cagaaacaat agtgaggatg gtatatggtg agagcctaag tacttaaaaa tggacatttg
      601 gtcgccaagc ctagaaatag gaaggtcaaa cgactatctc gagagagagt acactataag
      661 cgattgatag tggaaacggg ttgctcttag caggtaatgc tgaagatgaa gaaatagtct
      721 acgctcatat gaaagtatga ggtcactgga tttgaagtga catctataaa gtagcgttta
      781 taggtaaatg tttaaaactt gaaacatgta aatacttatg aaaacttata acttataatg
      841 attaagattg aaagatttaa attgaattaa atttactata aaaaattaaa aattcatagc
      901 tataatactc ttatatttgt aaagcatggt atataatgta tacaaaatta aagaatacgt
      961 gtatttgtta ataattaaat ttattgcatc tgttttttaa gactaaaaac ttaaatatat
     1021 gttggagtat agttgatatt tatgaacatc attaatttaa tatattagat ttttaagtat
     1081 ttatataatt caataagttt tatgtaacgg gagtttttat tatgaaagca aaaagtacat
     1141 caaaatttga attagacgga ataccaccac taaaggaagc aataccttta gggttacaac
     1201 acttaatggc aatggtaata ggtagttgtt taccagcgat attgattgca aatgcagctg
     1261 gacttaatca taagatgtca acattattga ttcaagccgc attagttttt gcaggattat
     1321 caactttatt acaattatat ccaatacctt tgtttaaagg ttttaaatta ggaggaggaa
     1381 taccagttat aatgggagct acagctatgt ttataggagc tggggtatct gttgcaagta
     1441 agtatggact accagtatta tttggctctc aaataatagc tggtatagta ataattataa
     1501 ttggttttgg tcttaaaaaa atacgattta tatttactcc agctgtatca ggaacaatag
     1561 ttgcatgtat gggtcttgga ctttttccaa tagctataaa aaacttagct gggggtatgg
     1621 gcaatgagac ttttgggaat ccaattaatt ttatagttgg aatatctgta tctcttatga
     1681 tattaggatt aaataagtat ggaaaaggat tatttaaaga tgcatcaata ttagttagca
     1741 taatttttgg atacatttta tcattaattt tgggtatagt aaatttttct tcaattcagg
     1801 agtttacttt ggttgcactt ccaaaaccac ttgcttttgg gctagatata agacttgaag
     1861 ttgttgtaat gttttcaata atatatttag ttgagatagc tgatataatg ggtgcttgta
     1921 cactttctgc ggtaggaggg cttaatagac aagtaacaga tgaagaactt tcttctgctg
     1981 ttttaggaaa tggagtactc tctacaatag gtgcattatt ttctgcaact ccaatgggga
     2041 tgtttggaca aaattctgca atcgtttcca atacaaaggt tgtaagtaaa tttgtactag
     2101 ctattggagg aattggatta tttttggcag gaatatcgcc tttattagct aatttaataa
     2161 gaacaattcc tccctgtgtg gttggaggag caacactcgt tatattttca actcttacaa
     2221 catctggttt aagacttgtt agtatggatg gatttaatca agaaaatagc atgatattag
     2281 gtctttctat ggcttctggt attggattca tggtagctcc tcaagttctt gaaaaatttc
     2341 caaaatttat agagacatta cttgcagatt caagtgtagt ttcaggagca atggtagcca
     2401 taataattca agctttatat atggtaaaat ttcctggaaa taaaagtgat aaggttgagg
     2461 ataaaaataa aagtttataa tgtaaaggtt taatatatgt gaattaaata tactacaatt
     2521 ataaatataa ataaaagatt tattaaaaac ttttgtgttt taataagtta atgatggggg
     2581 gataagatgg ttactataaa ggaatattca gttccacaga gtttagaaga agcatataaa
     2641 attttaattt ctaaaaaaaa taatgttatt ttaggtggat gtggatttat aaaattaagt
     2701 aataaaaata taggaacagc gatagattta aaagatataa atctaaatta tataaaagag
     2761 gataaaaaaa atattttgat aggtgcagat acttcactta gaagtttaga aattgataaa
     2821 actataaaaa attactgtag tggaatactt tcaagcgctg tttctaatat agttggtgtt
     2881 caatttagac aaggggctag aattggtgca agtgtatttt caaagtatgg cttttctgac
     2941 ttaataccag cacttttggt tgttggagcg aaggtgagat tatacaataa aggaatacta
     3001 gagttaagtg agtttttgga tagtgaatta agtagggata tattggtcga agtaatacta
     3061 ccaaaaaaag atgcaatagc tgtttttgac tcaataagaa aatgcacagg agatttttct
     3121 gtattaaatt cagcaatgct aaaagaaaat gatacttaca aaatagcaat tggggcaaga
     3181 cctcaaaaag ctagattagc acttgaagcg agtaatatat taaataaaga aaaagatata
     3241 gataaggcat ctattgttgc tagtaaggaa cttacttatg gaagtaatat gagagctagt
     3301 aaagagtaca gaaaggatat ggcaagtgca ttagttaaaa ggatgtataa tgctattgag
     3361 gagggaatgt ataatgatta ataaggggat aagaaagata gatagtacag caattgtcac
     3421 aggaaaacct ctatatactg aagacctcat tatgcataaa gatgttttga cattaaaact
     3481 acttagaagt ccacatgctc atgctaagat aaagaatata gatacatcaa aagccgaaaa
     3541 aattccaggg gttgtgggta tttatacgta taaagatgta ccacaggtca gatattgtgt
     3601 tccaggtcaa acagaaccag aagcatcacc ttatgataga gttttacttg aagatacagt
     3661 tagatttatt ggagatgaag tagctttagt ggctgctgaa gatgagaaaa cagcacttaa
     3721 agccttaaaa atgataaagg tagattatga ggttttaaag cctgtgtttg atgcaagaga
     3781 agcagaagga aatgaagttg ttatacatcc agaaaaagac atagtagagc catacccttt
     3841 tggattggat gcaaaaaata atatagtatc aaaagaattt tttgaatttg gagatgttga
     3901 agctaagctt aaagaaagtg attttgtggt tgaatcttca tacaaaaccc aaagtcaatc
     3961 gcattgcatg atggaaactc ataggtcata ttcatataaa gatataaatg atagaattgt
     4021 tgtagtttca tcattacaat caccacacaa tgtaaagaga atacttagaa aagtattggg
     4081 tataccgtct tcaaaaataa gagtaattaa acctagaata ggtggtggat ttggaggaaa
     4141 gaatatagca gcaacagata tatttactgc atttgttact atgaaaactg gtagaccagc
     4201 aaaatttgta tttacaagaa gtgaaacttt tgtaggttct aatagtagac atgaaatgtt
     4261 ttttgatgtt aaagttggtg cagataaaga aggaaattta aaagcaatag agctaaagtc
     4321 attaaacaat acaggtgctt atggtgggaa tggtgtctct gtttcttccg aatctggaca
     4381 caatacctta ccaatatata atgatattga tgctgttaga tttaatgcta gaacagtata
     4441 tactaatagg ttaccagcag gagcacttag agggtatggt gctacacagg gaatatttgc
     4501 aatagatagt gctattgatg aattggcaca taagattgga atggacccac ttgaatttag
     4561 aattaaaaat ataatagata ggtataccaa tggaaaagca gctgaagacg aaatattaag
     4621 ttgtgagctg agacaatgta tagaaaaggg aaagaaatta attaattggg atgataaata
     4681 tccatgtaaa gatataggaa acaacaaagt aagagcagta ggtatggcag tagcaaatca
     4741 tggttctggt ataccgcgtg tagatatggc aacagtcact ataagaatgg atgaagatgg
     4801 cacatataaa atattatctg gtcttgctga cttaggtcaa ggggctgata caattcaagc
     4861 tcaaattgct gcggaagctt taaatactac tgtagataga atttctattt atacaggtga
     4921 ttcagactta tgtccctatg atacaggagc atttgcttct tgtacgacaa cggttacagg
     4981 aaatgcaaca ataaagacag caaataaatt aaaagatata ttactttcag ttgcagagtc
     5041 aaagctagga tttaaaaaag aagatttaga gttgggaaaa gacagggttt ttgttaaaaa
     5101 cgataatagc aagtttgttc tattagagga attgggtaga gaatctgttg gaggaatagg
     5161 atttgctagt gatgatgtac cagtagtttt ggaagcaaca gaatcttttg gcatgttgaa
     5221 aaaaataaaa ccatttatag caggatttgc agagatagaa ttagataaaa atacaggtga
     5281 atataaagtc ataaattttg catgtgtacc tgattgtgga acagttataa atccaacttt
     5341 agctagaata caagtagaag gtggtgtgat gatgggtata gggtttgcaa tgtatgaaga
     5401 ccctatattt gataaagatg gtagattatt aacagatagt ttcctacagt ataaagttcc
     5461 tacaaaaaaa gaagttggaa atatattggt agattttgct gagaatttcg acccaggagg
     5521 tccttatggt gctaaatcat taggagaaat agttatacat acaccagcac cagctatagc
     5581 aaatgcaata tttaatgcta caggagttcg tttaagagag cttcctatga cttttgaaaa
     5641 agtttatatg ggtatgaaaa ataataataa acaattataa taaataataa gatgcataaa
     5701 agcaagtaaa tttaaattat tttgtaaaat aaagtaatta aatggacatt atatatggtg
     5761 ttttgtaaaa ataccgtata taatgtctag tagagtatat tctaaaggag gtgagttcat
     5821 gtcaaacaga ataaaaaata tacactatgg aactcagaga caacaattaa aaaacttaac
     5881 taaaaaagaa tatcaagctc tacgagagat gtgcttctta gctaagaact tatataatgt
     5941 tggtacatat aatgtaagac agcattattt caatcaagga gaacatctta actatgaagc
     6001 taattatcat gtatgcaaag aaaatgagaa ctataagctt ctcaatagta atatagcgca
     6061 acaaatatta aaagaagtag acagttcgtt taggtcattt tttggattaa ttaaattagc
     6121 caaagaagga aaatatgact ttagagggat aaaatcacct aaatatttag ataaagaagg
     6181 attttttagc ataataatag gtcaaataag aatcaaagaa gatgggattt taaatgtacc
     6241 tatgtctcca gcatttaaaa aactatatgg gaaagtaagt ataaaagttc cacaaaatat
     6301 actagagaaa aaaattaaag aaataagaat aatacctaaa cataaagcta ggttctttga
     6361 aattcagtat tgttatgaaa taccaaaatc agaggaagtt tcaaatcaaa aaaatgcact
     6421 agctatagac ttgggtttag aaaatttatg tacatgtgtt accaatttag gtgatagttt
     6481 tatcatagat ggaaaaaggc taaaatctgt gaatcagtgg gcaaacaaac agaatgataa
     6541 aatagaagcc ttaaaattaa aaaagaatat aactgcaata acaagaaaac aatttaaagt
     6601 ttggaataag agaaataatc aagttaaaga ctatgtaaat aaaacctgtt tttatataat
     6661 tgattattgt gtaaaaaata atattggaaa tttaattgta ggatataatg ctaagttaga
     6721 aaaaagtgat aatatggaga gaaaaatata tagaaatttt ataagaatcc catttggaga
     6781 gataaaagga aaattagaat atctgtgtaa aataaaaaat atcaatttta ttaggcaaga
     6841 agaaagctat actagtaaat cagatttttt tgctgatgat tttctacctg atatcaatgt
     6901 ggacaatgtt aaaaaatatg actttaaagg ggaaagaata acacgtggac agtacaaatc
     6961 cagtgttgga atcattataa atgcagatgt aaatgcgagt tttaatatac taaaaaaatc
     7021 acaagtagtt aatttatcta atttacagaa taatcctcaa atattaaagc aaccacagag
     7081 agtaagaata tcttaattat atttaaaaga taaactgata gcgtaagctt acagcttagc
     7141 ggcgaattgg atccgccttg ttgtttacaa cagaatctat ttactttcga gtttatggaa
     7201 tttcaaaaat cacttttact aaaaaggcct aactataata attaagagat tagaaaactg
     7261 gttataagag gtgaaagtgt ggaactatca aatataatag aaaagaatga aataataact
     7321 gtagttggag caggaggaaa aacttcgttt ataaactatt ttgcaaattt ttatagaaat
     7381 aaattgaaag ttcttcttac aacaacaact aaaatatatg taccaaacga ttatgacaat
     7441 ataattatta caatagatgg aactgttatt ccatctattt gtcatggaat aacagtctgt
     7501 ggcagttata taaataatga aaataaatta gtaagtattg attcctctat tttggatgaa
     7561 attgtagacc aatttgattt agttttaata gagggagatg gctcaaagag aaaaaaactt
     7621 aaaggttgga atgctaaaga accagtagta tatcatagaa ctacaaaaac catagggata
     7681 ttagatataa catcatttgg aatgaatatt aatgaagaga atatacatag agtagaaata
     7741 tttaaaaaaa tagcaaattt agatacaagt agtattaata gtagttctac agtaagtatt
     7801 gaaaacttaa aaaatatagt attaaatcca aatggattat ttaaaaatta ttctggaaaa
     7861 agagtattat ttattaataa agttgaaaat gaaaaatata aaaatttagc tataaaatta
     7921 atagaaaata ttaaagaata tgaaagtgaa atagagatat tttatggaag tgtaaaacaa
     7981 aaattttgtg taagatattg aagtattaaa atagtttata tgatattata tatatagata
     8041 atatcaatag agatattata aaaatcatag aactatcaat atattataaa taccaaggat
     8101 gatatatatg ataaatgcag tgataatggc atcaggcttt gccagaagaa tgggagcaaa
     8161 taaactttta ttaaattata aaggctgttc tataatagaa catgtattta gagaagttag
     8221 caagattgat tttcatcaag ttgtagtagt aagtcaatat gaagaggttt tgttattgtg
     8281 taataaatat ggttttgaat atatagataa taaaaatgca aatataggtc aaagtgaatc
     8341 tataaaatta ggagttttaa atagctcaaa atgtgatggt tatatgttct ttgtaggaga
     8401 ccagccattt attgataact tatatataga taaaattata aatactttta aattagatag
     8461 agattttata gtaattccaa gatacaaaga tatgtgtgga aatccagtca tattcccata
     8521 tagcaagaaa gaagaattgt tggcacttaa agaagatgaa aaaggtaaaa ccattttaaa
     8581 aaattcatca aaaataaagt atgtagaagt tcctaaagaa atgttatttg atatcgatac
     8641 aaaaatggac tatgagagaa ttgggggtaa attatgaagc taataaaaac tatagatgct
     8701 gttggacaag ttttatgtca cgacataact caaataatac ctggagaatt taaaggaaga
     8761 aaatttaaaa agggtcacat agtaaaagaa gaagatattc cagtgttatt atctcttggt
     8821 aaagataatt tgtatgtatg ggaaaagtca gaaggaatgg ttcatgaaaa tgaaggcgca
     8881 ttgtttttaa aagaactaac tgctggagaa aatttagatt ttagtgaaat aaaagaaggg
     8941 aaaatagatt ttatagcagc atgtgatggt ctattaaaga tagatgttga tgcacttttt
     9001 gatttgaatt gtgttggaga aataatgatg gctactcttc ataataattt tatagtaaat
     9061 aaaggtttaa aagtagctgg aacaagagtg atacctttag ttatagatga gaaaaaatta
     9121 gaagatgcaa aaaaagtagt tggtaataga aaaatagtaa atgtagtacc ttttaaacct
     9181 aagaaagtag gaattgttac tacaggaaat gaagtgtttt atagcagaat tgttgataaa
     9241 tttggaccag taatagaaga aaaagtaaaa ggctttggtt gtgaagttat aggacagact
     9301 atatgcccag atgataaaga aattataaag agtgcaataa aagaatttat aagtcaagga
     9361 gcagaactta tctgttgtac tggtgggatg agtgtagacc cagatgatgt tacgccaaca
     9421 gctataaaag aaactggagc tgatttagtg acatatggtt caccaatatt accaggagct
     9481 atgtttttac ttgcttatta cggagaagtt ccaattatgg gtataccagg atgcgcaatg
     9541 tatcacaaaa ctacagtatt tgatatagtt ttatctaggg ttttaattga tgaaaagctt
     9601 gataagtatg atatagctag atatggtcat ggtggccttt gtatgaactg tgatgtatgt
     9661 acttatcctg catgtaattt tgctaaaata taaagttatt aaaaaagagt atttacaaat
     9721 taagtactct ttttaaatat gaaacaaaac agctagaatg atgttttaaa aaaagcatat
     9781 ttatttagaa attataataa cttattttca atgtgtaaaa gaatgaataa agttgcaatt
     9841 tgatttcttt ataagtataa tataagagat tgttaatttt tataaattta caatttttag
     9901 tattatttta ataaatattt cacaaattta gtataaaaaa tatactattt acgtgataat
     9961 atataataaa aatgttatca aagttttatg ataaaataaa ttatgagcaa aaagaaagga
    10021 aaatatatta atgttacaag ttacaggtgt tggacttaga tttggtgata aggaactatt
    10081 taaagatgtt aacttaaaat tcactaaagg taattgctat ggaataatag gagctaatgg
    10141 tgcgggaaaa tctacttttt taaaaatatt atcaggagaa atagagccta atacaggtag
    10201 tatatctata actgataaag aaagaatgtc agttttaaaa caggaccact ttgaatatga
    10261 agaagaaact gttttaaatg ttgttataag aggtcatgag agactttgga acataatgaa
    10321 agaaaaagat gcattgtata tgaaggaaga ctttaatgaa gaagatggga taaaagctgc
    10381 cgaacttgaa ggtgagtttg cagagcttga cggatgggat gcagaaacta atgctgagaa
    10441 aatacttatg ggtcttggca taacgaaaga tatgcattac aaacaaatga aggaactagt
    10501 tggtggggaa aaggttaagg tattgttagc acaatcactt tttggtaagc ctgaaatact
    10561 attaatggac gaacctacaa accacttaga ttttaaatca ataaactggt taaataattt
    10621 tataatggat ttagaagaat ctatagttat aatagtatca catgatagac atttcctaaa
    10681 tcaaatatgt actaatatag ttgacgtaga ttttggtaaa atacaaatgt acgttggaaa
    10741 ctatgatttc tggtatgagt caagtcaatt ggctttacaa ttggctaagg accaaaataa
    10801 aaagactgaa gaaaagatag ctcaattaaa ggaatttata gctagattta gttctaatgc
    10861 ttcaaaggct aaacaagcaa catcaagaaa gaaacagtta gagaaattag aagttgaaga
    10921 tatacaacca tcaagaagaa gatatccata tgtaggattt acacctgcaa gagaaattgg
    10981 gaatgaagtt ttagaagtac acaatttaac taaaactata gatggggtta aggttcttga
    11041 taatgtgtca tttagattgg atagagatga taaagtagta tttatgggag atgaaatagc
    11101 tactactgca cttttaaaca tagttatggg agaacttgaa ccagacagtg gagagtataa
    11161 atggggagta actacttctc aagattatct tccaaaaaat cataataagt tttttgacgg
    11221 agtagaatat tctttggttg actggcttag acagttctct gaagaaaaaa gtgaaagttt
    11281 tataagagga tttttaggta gaatgttatt ctcaggtgaa gaagcattaa aagaagctca
    11341 agttatatct ggaggagaaa aggttagatg tatgcttagt aagctaatgc tttcaaatgc
    11401 aaatgtactt gtattagatg accctactaa ccacttagac ctagaaagta taacttctgt
    11461 aaataaagga cttgaaaagt tcccaggagt gcttttattt acttctcatg accatgaatt
    11521 tatatctact atagcaaata gaattataga gataactcca aatggaataa tggatagaaa
    11581 aatggacttt gatgagtatt tagaaagtaa agagatacaa gaccaattag ctaaaatgta
    11641 tggaaaagat aaataattat tagtataaaa ccttttaata tttgagacat gctatgcatg
    11701 agtgtgtaaa tttttaatca aatattagga ggttttttat attgtttaaa catgtttatt
    11761 agcagaatag agtaatattt tgtatattaa gaatttaaat ataatatagt agatgatata
    11821 ataaaaatat ataacatttt ggggggaata gattatggtt aagaaaaggt tagtagctgt
    11881 agtaattagt ttagctatga ttagtgtagg gaatttttca tatgctcact caggagggca
    11941 taattcatca ggaggtcatc ataataaaaa taaatcagag ttaacacgtt attattgtgg
    12001 aggacattca gcacatcaac acaataaagg tttttgtcct tatgctagta caaaagaaaa
    12061 ttctggagta acaatatctt cagtacataa cgaaaaaaat cataaaaact attttgcaga
    12121 aaaagggtat aataaaggtt atgaagatgg gtgtaaaggg ctttataatt taagtgtcac
    12181 agaagccaaa tctgataatt tatatgaaaa agagatttta aaaagttcat atgaaaaagg
    12241 atatttaatt ggatacaatg aatataaagc aaaaaaagta gatgatgtaa tgaagaagat
    12301 gttagatatt ccaatgttag caagtatata ataatatagt atttaaaaag atattttcta
    12361 taagggaata tcttttttat tgtaaagtat aatttttcgt aaaaaagtat aataactgag
    12421 gcgataaatt aaaataaaac agattattta agtagttatt aatttgagat agatacattt
    12481 aatattaatg aatctatctc aataagctaa gctaatttaa agctaagata aattaatatt
    12541 aattagcatt aaataaactt tcagaaattt tgtcagaagc ctctttatcc atttctttaa
    12601 gtacatgaga ataaacgctt agagtagtac ttatatttga gtgtcctact ctctcggata
    12661 taacctttat aggtacttta gaatttataa gtaaagtaac atgtgagtgt cttaaatcat
    12721 ggaatctaat atgaggtaat tcattctttt ctaaaaatct tttaaatttt ttactcataa
    12781 catcttcagc aataggttca catttcttat tgaaaaataa caaattatta ttatttctaa
    12841 tggaagattt taataaattt ttattctgtt ttattttata ttcttttaat aaagacatta
    12901 attctttggg agaagatatt tttctaacag agctttctgt ctttggctct ttgagaataa
    12961 cagaaccatt aattcgggaa gttatcttat ttactgttat tgtattttct tcaaaatcaa
    13021 tattatccca agttagacct aaaacttctg aaattctaag acctaatcca atagccaaat
    13081 taataggtaa ttccatttct gtattttttg ctaaatctaa taattttatc atttgttctc
    13141 tatcataaat ctcattttta aattttttat atcttggagc ctctatacta tccataacat
    13201 tttcttttat taatttgagt ctataagctc tttttattgc aagtttcaat atatttatat
    13261 gaatcttaat tgattgagga tttagtttat aagatagttt atctatgtag ttttgtatgt
    13321 gaaccggatg taagtcttgt attttatatt ttccaataga tggattaatg taattcttac
    13381 aaatactaac ataattatta tatgtagcta aagatatatt ttcactatat ttatttagaa
    13441 aatctaatag aaattctgac actgtaattt cgcttggtac taaaaaacca tcattatata
    13501 agctttcttt tacctcattt agtcttttac ttgcttccct ttttttatca aaagtaccca
    13561 tatttttttg ttttctttta cctgtctttt catcttcaaa ttccaggtaa acaacatagt
    13621 ttttatttct ttttctaata aatgcattca tatataaccc accaatcaaa attttataca
    13681 tttattataa catatatata tcacaaaata attttgaa
//
LOCUS       DAYAOY010000109        13675 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYAOY010000109 DAYAOY010000000
VERSION     DAYAOY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.109"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(708..1337)
                     /locus_tag="ACGI6B_003315"
     CDS             complement(708..1337)
                     /locus_tag="ACGI6B_003315"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HFG9752730.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1416..2378)
                     /locus_tag="ACGI6B_003316"
     CDS             complement(1416..2378)
                     /locus_tag="ACGI6B_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752731.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3140..6655)
                     /gene="cbpA"
                     /locus_tag="ACGI6B_003317"
     CDS             complement(3140..6655)
                     /gene="cbpA"
                     /locus_tag="ACGI6B_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HFG9752732.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAGDAEGDTNAGDAEGDTNAGNAEGDTNAGDAEGD
                     TNAGNAEGDTNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEINVD
                     FKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTL
                     TPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVP
                     EGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIII
                     NGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGGGYYTT
                     TKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVT
                     GGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMN
                     KISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKGSEVSV
                     NLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEED
                     VEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTKQV
                     DAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKP
                     TDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTAS
                     IEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDIN
                     IDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDG
                     NPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDSG
                     NGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPD
                     PDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINID
                     TDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIM
                     LDLALMFISLFFLIKNLTNKYLRRK"
     gene            6924..7496
                     /locus_tag="ACGI6B_003318"
     CDS             6924..7496
                     /locus_tag="ACGI6B_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFG9752733.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(8509..9171)
                     /locus_tag="ACGI6B_003319"
     CDS             complement(8509..9171)
                     /locus_tag="ACGI6B_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752734.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(9188..11743)
                     /locus_tag="ACGI6B_003320"
     CDS             complement(9188..11743)
                     /locus_tag="ACGI6B_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9752735.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(11983..12171)
                     /locus_tag="ACGI6B_003321"
     CDS             complement(11983..12171)
                     /locus_tag="ACGI6B_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFG9752736.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(12183..12362)
                     /locus_tag="ACGI6B_003322"
     CDS             complement(12183..12362)
                     /locus_tag="ACGI6B_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752737.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(12367..12624)
                     /locus_tag="ACGI6B_003323"
     CDS             complement(12367..12624)
                     /locus_tag="ACGI6B_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752738.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(12597..12737)
                     /locus_tag="ACGI6B_003324"
     CDS             complement(12597..12737)
                     /locus_tag="ACGI6B_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752739.1"
                     /translation="MVIAISLTLGLITGCTNKVSGTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(13004..13210)
                     /locus_tag="ACGI6B_003325"
     CDS             complement(13004..13210)
                     /locus_tag="ACGI6B_003325"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9752740.1"
                     /translation="MCKYLDNAIEACDKIHDKSIDKYIQIKGTISKSYFVIKCENSKI
                     NSIRKNKSLLLTDKKDKLVHGIGI"
     gene            complement(13195..13326)
                     /locus_tag="ACGI6B_003326"
     CDS             complement(13195..13326)
                     /locus_tag="ACGI6B_003326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752741.1"
                     /translation="MDIIINEKSDVSEKRKIKFICDINFSKVNFIKPIDVSNIFANT"
     gene            complement(13386..13631)
                     /locus_tag="ACGI6B_003327"
     CDS             complement(13386..13631)
                     /locus_tag="ACGI6B_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9752742.1"
                     /translation="MIPRLILASSLVIIIVFIKIIKENKLKLQNEVIKNKLDMQYSHY
                     LSIQESHAKVKKLYHDINNHIYCIENLKYKKGKLMGM"
BASE COUNT         4410 a         2337 c         1435 g         5493 t
ORIGIN      
        1 aagattttat aataaacatt acaatacagt actaaattag tttatttcat ctatcatctc
       61 aatcatatca tttactaact attattctaa tgagcatata acattgaaac ttataattat
      121 acaacctaaa tgcctcccaa tgtaccctca aaacatcatt tcctgtaact gaagtataat
      181 tggtatattt tatcactgaa ataaaaaaac actagtcaat aaagactagt attttcaagt
      241 attttatcat tcttttttta attattggag acgaatcatc agaataatgc ccataattta
      301 tctatatgca aattctaaat ttaaaataaa gtttcagata aatttactat tctatgctat
      361 gatacaacag aaaaaactga aatctatgag tggtagcttc atataatcct agttcctaca
      421 gtaatcaaaa ctgaggttta ttctatatgt aactaatgat aaattttatt ataggagtta
      481 ttagatctat ttgtgttact ataagtagta attatatata tgataatttt ttaacacttt
      541 tctgttggct atatagaaag tggtttagaa tttaaattta aataaaattt aaattcacca
      601 aaaagaaatc tatattagaa accactactc tattccacca gattgtaatt ttttgttctt
      661 tatatcacaa tgctatacca tattaaattg aaaaataagt tttaaattta tttgttacaa
      721 ttcattaatt caaaaaaata ttttgagtca tgagttttgt aaagatttag ccatgaattt
      781 aatgcttctg attcaaaaac atctaaaagt ttcaatactt gaaacgtaaa ttccaacgga
      841 gcaattcctg aagccgtaac taaattttca tcactaacta cagattccgt tttataatat
      901 ttttctccag agtaatttgg tattaccatt ttaagaaact ctaagtcatt acttgtatga
      961 tttctaaaat ctaatagccc ttttttagct aggcctaatg tagctccaca tatggctgca
     1021 actatagaat cttgtcttaa agcttcttta actttttcta atattatctc atgattcata
     1081 gataaccaag tatttccccc tggcaaaatt aataaatcct tactttccat attaaactca
     1141 tcaactgtaa tttcaggtaa aacttttaac ccacccattg ttataatagg atttttatca
     1201 attcctacag taattacttt tagtggcttt aatccttttt taaaatatct tcctgtattt
     1261 aattcagcag ttaaatatgc cacttcccaa tctgacatag tatcaaatac atacatatat
     1321 acttttctat ttttcatctt ttttactcct tatgtaatta atttatatat ctaattataa
     1381 aataacttaa ctgacatatt cagtcagtta agctattaaa tttgataata atcaattaat
     1441 tctagtaaat cttcaattat tttcttccta aggcttagtg gttcaattac ttgtattgtc
     1501 tttctataag gtaaaagtat atttggtatg tacttgcata ttacttcctc ttcaagcata
     1561 aatattattt tattaggtgt tctttctttc aagtagtgcc ctaaaaacca atgttggctt
     1621 aaactatcta aagaatctat actcccttct ataactaatt gaattatatt ttgttcatct
     1681 tttatttcag gtataattcc ttttataaaa aattcacttg ctgaaaattc tgtcggtctg
     1741 ttaaatgtat ttgatgtttc tatgatttca ctaatcctat ccactctgaa actacgaacc
     1801 tcatttctca tattacaaaa tgcaactacg taccaattat tattccagtg aacaattcca
     1861 tatggattta taattctata ttttgatttt actttacgac ctgcatgata gttgatttct
     1921 acagatagct cctttaatat actattctcc aattttttaa gtactgattc tatagacact
     1981 ttcccaattg gttttattac ttcaagggct cttaagtggt ttgttaaaat atcttcttgt
     2041 ttttgatttg aatacatttt taactttaat atcgatttat ctaatgcttc gtctaagaag
     2101 tatccttctt ttttagcaaa tagtgctgca tgaagtagag atttttgctc ttctatatca
     2161 aaaaaaagag gtgctttagt aaaattattt aaaaggctat acccaccatt atgacctgta
     2221 tctgaaatta taggtactcc actcgcagaa agtacatcaa tatatcggta cactgttctt
     2281 atatttattt ctaatttttc agctatctgt tttgctgtaa tttttttgtc agagtttaac
     2341 atccacagta tagacatcat attgtcaatt ttagccatgt agtttcacct ctttctattt
     2401 gttatataaa gttattatat aactttttga ttgatttttt acaaaaaaac attagtaaac
     2461 tgctaataat ttatactaag atttatttca aatatttata gatagtcttt ttctatagtt
     2521 aagttatcca ttaaattaca tagtagtata tctattttag ttgatatatt ttcagctacc
     2581 tcccttgaat attattacaa taagtctaaa tcttctttct tccataaata tattatgagg
     2641 aaagactatc atctttaatg gtttaatagt ataaaagata ttaatttaaa aaaatctatt
     2701 tttatatcag atgggtattg ttttttataa aactgaaaaa atcacctaat agaatctatg
     2761 attttttaac ataacctcca taaattagtt ccaattatca cttcaaattc aaatttttat
     2821 ctgaagaata tacaatcata tttccatact caacataata ttgagtgttt ggcttgtatt
     2881 tacaaattat tattctaaca aaaacaacta tacatagatt aaaatcatct tctaaacata
     2941 actactaaaa ttgaattaat gatatcaatt ccattaatct cttttaataa aaaattctct
     3001 tttctatatg aaaattattt caaaactttt aagttaaata caaaaatgac tataaataga
     3061 tttttattta cacaaatcta tttatagtca ctatagttta tccaataaaa taataaaata
     3121 aatatgtaca caataaatgt tatttacgtc ttaagtattt atttgttaga tttttaatta
     3181 aaaaaaacaa gctaatgaac attaatgcta aatctagcat tatattgctt tgatctcctg
     3241 tttgtacatt ttgacctaca ttactatcta aactttcatc tgtatctata tttatatctg
     3301 gttttccatt cccatctgta tctatattta tatccggttt tccatctcca tctatatcta
     3361 tatttatatc tggttttcca tctccatctg tatctatatt tatatctggt tttccatctc
     3421 catctgtgtc tatatttata tctggttttc catctccatc tgtgtctata tttatattcg
     3481 gttttccatc cccatctgta tctatatctg gtttgctatt tcctccatta tctgggtctg
     3541 gtttactatt tccaccgtta tctggatccg gtttgctatt tcctccatta tctggatctg
     3601 gtttaccatt gccaccgtta tctggatctg gtttgctatt tcctccgtta tctggatccg
     3661 gtttgctatt tcctccatta cctgaatccg gtttgctatt tcctccatta cctgaatctg
     3721 gtttgctatt tcctccatta cctgaatctg gtttgctatt tcctccatta tctggatctg
     3781 gcttatttag atttggatta cctgtatcat aacctcctga gccagtatct acatttttat
     3841 caggcttcca ataatcatct actccatcat tatctgtatc tattgctgga cgattataat
     3901 ctgtatcagg gtttccatct ccattggtat ctatattttt atcaggtttc caaataccat
     3961 ctccatttcc accttctgta ctaggcttcc attcacctgt attatcagta tctatgttaa
     4021 tatctggctt ccaatcacca ctcgtatcta tattgatatc tggccttcca tcaccatcag
     4081 tgtctatatt tatatccggt attccatccc catctacatc tacatttgtc atatctaact
     4141 ttaacttctt ttcatcctca ctaccttcaa tgtaatttat atcatctcca atatatttta
     4201 cagtaatagt ttgttcagta gtaaaaatat gatgattgct atctaaaggt ctccattcaa
     4261 tagatgcagt actataatta tgactctcac tacccttatt atgagttttt aattctactg
     4321 gatctttata ttcctttcca ttaatataga attgtacgta acctcttgga gaggcacaat
     4381 ctggtccttc tttcttatct ggtgaaatag taacatttaa tgtaaacgta tctgttggtt
     4441 taaaaacatc agttttagct ttagactctt ctacaactaa tgaagtttct gtatcagcag
     4501 gaataatttc tgccttataa tatgctctat gcccccaata agcatcctta aaaggatata
     4561 tactcgaaaa ctgtgttgat gtaatgctta attgatattt cccagcatca acttgttttg
     4621 taagatcatc acttcctaaa tttatatctg gttccatcgt ttcctcattt aaacgttgag
     4681 aactaatagt cataaatttt ttatctgtga gtttttcacc tttaggagta attattggaa
     4741 taccaccatc tttaaatatc caatctaaaa atgatttttc tacgtcttct tcaaatggta
     4801 atccatcata tcttttaata atcaaatatg gtggagtatc atcaatagta aattcttcat
     4861 attgctttaa aaattgagaa cctgaaccta tatctttaat ataaaaatca tctggtttta
     4921 gaacttctcc agtaatttta tctataacaa ctaaattaac agatacttca gatcccttaa
     4981 attgatcaga gaaccataag taaagaatcc cttgagcatc tgtataacta ggtgctccat
     5041 aaggataaat ttttccatca attagcattt taaaatcatc aactagtgta gtactaactt
     5101 tatcttgccc taaatacgtt agagatattt tattcataaa aacttttgtt ttcttttcta
     5161 aatcaccgta tgcaactgct ccacctaaac ttttgaatgt tgttgctttt aatgaaccac
     5221 ctgttactat aacatctccc gttgtagcac ttaggtcaaa aatccaatct cccactcctg
     5281 tcatataagg taaaatcgtt cctccagtta caagtatacg atatcctcta tttgttcctg
     5341 gattaacaca tccatcccca aatgcactac catgtgcacc tccatatgaa taggaaagtc
     5401 caccattaat tgtaatattc ccactataac cagaacctgt gctaggatct actactgagg
     5461 gacttaatgc attctttgtt gttgtatagt atcctccacc tattccagct ccatggtatg
     5521 cacctttagc agttataata cctccattga ttgtgatatc tccaccacta ccattaccgc
     5581 ctccacctat tccagctcca tatcctgtat tacgactcga ttcttgacct atagcagtta
     5641 tagcaccacc attaataata atatttcctc cactttcagt atttccacca ccaatacctg
     5701 cagaataata atttccaaca acttttaaac ttcctctatt attactatct agtagtgtaa
     5761 ggcgatctcc tttttttaac ttgtctccat tttctagagt tacatctcta ggaatttttc
     5821 caccctcagg aactataaac tctccttcaa tatctctatt tacaacagaa tcatcaataa
     5881 ttagagtagt accttttcct gctctcatac ctggataata tacatcacct gactctagct
     5941 tgttttcact accatcttgt aatataacat gtgcatatgc accaccatct ttatctggtg
     6001 ttaaagtaaa tgggctatta gatgcagttt taatatttac tccatcaaat attaattcag
     6061 cttttacatc tttaggtata acaatcctat gtgttgttgg agtatttgga ttgttatttt
     6121 gaattgtcat ttgttttcca ctaagaatag ttaaaacgcc acttgcaaat ttaaaatcga
     6181 catttatttc tccaccacta attttaaagt ctcctacaga cgtaggctct tctgcagtgg
     6241 cttcttcttc tacaactact gcattagcat ctccttctgc atctcctgca ttagtatctc
     6301 cttctgcatc tcctgcatta gtatctcctt ctgcatttcc tgcattagta tctccttctg
     6361 catctcctgc attagtatct ccttctgcat ttcctgcatt agtatctcct tctgcatctc
     6421 ctgcattagt atctccttct gcatctcctg cattagtatc tccttctgca tctcctgcat
     6481 tagtatctcc ttctgcattt cctgcattag tacctccttc tgcatttcct gcattagtac
     6541 tttcttctat agtatctgcg aaagtatttg acgcattaaa tgatgctact ggtccaagtc
     6601 ccattgagat acttaaaatc attgatagtg ctttcttttt acatttttgt tgcataaact
     6661 ccccctcccc ttaataattt ttaatataca ctgtatacat ccttatatac actctaataa
     6721 ttcttaatat acgctgtata cattctaatg cacactctaa taatttttaa tatatactta
     6781 aaacttatat ttgtcaatga atatactgtt ttttcaacct tatatattat aagtattcat
     6841 tatttgctaa ataactacaa taaaaatttt aattatataa aatttattta attttttata
     6901 taataatact aaggggtaat taattgaaaa aactagtaaa aatattaata aatattattt
     6961 gcattattac gctaattttc tctagccttt cgatttacat aaaattatct gaatataaaa
     7021 aagctgatga agtatatacg gaactcagag aaaacactat caataatagc aaacatcaag
     7081 aactttatga taaaaataat gattatagat tctggcttaa aataaacaac gtaaatatag
     7141 actatcctgt agtacaagga tataacaatg atttctacct aactcatgat ttttataaaa
     7201 attatcttcc tttaggtagt attttcatgg attatagaaa taattttgaa aatgataaga
     7261 gtttaatagt ctatggtcat tatatgaaaa ataaaactat gtttggtcag ctagaaaact
     7321 atactgatga agcatttttc aaagaaaata acttagtaga aataaattat aaagtacaaa
     7381 cttatactta tgaaattttt tcagtatata ctgctgactt aattaacaga gattatttaa
     7441 gtatacactt taatagtaat tatgagttta aagattattt gaattatata acctaaaaat
     7501 cagtacacaa atctgatata aaggtagata tttcagataa aattataact ttgtatactt
     7561 gtaactatga atttaaagat gctcgtacta tagttcatgc taaattaatt tcaaaaaaag
     7621 agactgataa gtaggtttgt gtaaatgcag acttacctac agtctttttt gaacgtaaaa
     7681 ttagtaagta tggaataata ttcatattaa acttttgtag gcaaagaaat gaaaaatcaa
     7741 agagaaataa ttattacaga tttagattac caagctgaat taagaaagtg taaaactatg
     7801 gaggatgtag ttggtgaaga tagtatataa tagtttaact tataaattat cttggtgata
     7861 ttaatgaagt ttcaaagtat atattattta ttgatataaa taatattaaa tctgttgcta
     7921 acagatgtac ctttgtttgg agacaaacta tttaagaaat ataactaaaa tgttgaagta
     7981 gctacctgac tttattttga aatgcgaaca agattttggc attaagataa tttagaaaga
     8041 atcactggta cacaaggatg tgaacttaga atgaatagaa gtattcaggc ataaggagct
     8101 ttcacccaaa taaaacatag tatgaacttt aaaagaattc taatcagagg caatcaaaat
     8161 gttctttcat agtttatacc tgtagtgtta gacatagtat ttataagttt cacaaataag
     8221 ataatcatta ataaatgtag tatgtatatt catcctctca aaaatatttt ttaatcttac
     8281 actttgtaaa ttattctatt ttaaaatggt atttttatac ctttatttct tatgaccgtt
     8341 ttcaagctca aattttgcat aaatataaat taatgaaaag caagtaaaaa aagaactgtc
     8401 gcacagcaat ttaaaattac tcattcgaca gtccttattt cgtggattta aatagtggag
     8461 aagaatcaca agaataatac ccacgattaa acctttatac aatacatttt atatgacttt
     8521 accatcacta atagttatta tacaatcagc catcttagct atttcatcat tatgagttat
     8581 cataaccaac gtctgcttgt attcatttac acaatacttt agtaaattca tcacctcatt
     8641 agttgtttta ctgtctaaat ttccagtagg ttcatctgca aaaattattg atggcttatt
     8701 ggcaagtgat cttgctattg caactctttg ttgttgacca cctgataatt cattaggaaa
     8761 tttattaatc tgactttcta atcctaactt agttattaaa tcattaatat aattattatc
     8821 tatctttcct ttagcaatta aaataggaag aactatatta tcataaacat tgataacagg
     8881 tattagatta tagttttgga atataaaccc aaattcttta cttctaagat tagatagctt
     8941 ttcatcactt aaagtatata aatctaaatt atccataaat acattaccta aagttggttt
     9001 atcaagccct gccatacagt gaagtaatgt gctttttcca gaaccacttt gtccaattat
     9061 tgcactaaat ttatggggtt ctatctctaa agtaattcca tcaacagcct ttatttcatt
     9121 ttctcctttt ccatatattt ttttaatatc tataactttt aacatcttca tacatttacc
     9181 tcccaaatta ttcattctct tttatacctt caataatatt catactttca aaatctctat
     9241 tacttacaac tactgatatt aaacatacaa atacagatat agcaaagaat agtaaagatt
     9301 ccttaattgg gaatgtaaaa gacataaaat tctctattcc atagtttata tacgcatcat
     9361 tattccaatt tgtaaattta actagattta taaatgatgg aatcatagct attataaagc
     9421 taaaaactgc atatgctaat gcttcatata taagcatttt tttcatattt ttaagactaa
     9481 cacctaatgc tctaagcgtt gaaatttctt tttttctaat tagtagattt gttcttatag
     9541 tacagaaaat attaattgat gctattatta aaactagtgc aattattgct atttgtgatt
     9601 taatgtaact tttatatata ctttctgaca tttcatgata acttaatcta ctattaaata
     9661 gagaccctgg tatacttctt gaaattttct gtatctcatt attaacaatc tcatcatatg
     9721 gattttctaa atttatgcct aattgagtgt atttttgttc tcctgttatg tttttagagt
     9781 gaatattact tgttataatt tctagatctg gcataaaatc gccatctccc atagcaatcc
     9841 attcatcctt taaaattaca gccactctta ccttcatctc tttataaaca attgtattat
     9901 tttcttcaac aggaattttt acagttatta tgtctcctat atttaattct ttgaatatag
     9961 gctttatagt atgatcatca actatatcat aaaaataatt atatactgct acatttggat
    10021 atccatcagt gtccttttta atatcatcta agctaccttc ttcaacaaag tcatttaact
    10081 tttcatctat ataatcatca tcataaagct ttaaaactaa tttatcttcc attatctctt
    10141 ttttttctac tccattatag gttgtaaatt ctttgtttat atccttatta ttaaaagtaa
    10201 acattccatt tgttatttga attttatgta tacttttaac acccttaatt ttttctatat
    10261 tctctacatc actatctgtg tattttgata tttgcgggtc tgcatttact ccaaattgca
    10321 ttgttatatt atatctattt tgcatatctt caattttact gttgacctct tcttgcatac
    10381 ttgagaatgt agtaatgaat atatatcctc ctaaagcaat agacaacata gatatagcag
    10441 ctctaacctt atttcttccg atatttttaa gtcccataaa tccataaaat ccaaatattt
    10501 tgcttactag tctgtctatt ccaagtctac ttctgtgatt tttaattttg tctgtattta
    10561 ttattgattc aataggtgat atcttgccga ttttaaaagt tggtataata caagatataa
    10621 aaacagaaat cattccaacc aatatagctt ttataactat ttcattattt atatataatt
    10681 tagggttaag accagcttca ctgtataaaa cttcgttata attactaatt cctatatatg
    10741 aatatgtaac cccagctact aacccaagta gtatacctat tattgttata attaatgctt
    10801 gatagattat catacacctt atgtttcttt tctttgcgcc catcagtcta agcagtccca
    10861 tttcttttac tgattgattt agtgtaatat tgaaaatatt aaatatgaaa attatagata
    10921 ctgtagttat taaaacattt cttttaaaaa cattcggatc attcttatca tcttctacat
    10981 aacgaagttc ccttacaaga ggtacatttg gtagaaaact tttttgtccc aacttatatt
    11041 ttttcataag ataatcacat tcaccttcaa tatttctcca tccactttta aaacttaata
    11101 tactattata actaatcgca tcatgaggta taatgttatt tgtttcatca ttgccataag
    11161 ttactgcttc gtattgccaa accacatcat agtacccttt tggtttttcc ataactccta
    11221 ctattttaaa ttctttattt atagaaaata attgattact tccgttagta tctttatagt
    11281 tcttttttat tgttaaattt aatgtagatc ctactccttc acttaaattc attgcttcta
    11341 gtgctttttt ctctaatact atttcattgt tgtttttagg tagtctacct tctattattt
    11401 tgttgtatct actttttaga tactcttcgt ttgatgaagt taatataaat gagtttcctt
    11461 ttttatctga aatacgtcct agattttgta ccgaattacc ctttgatact ttttcatcag
    11521 tttctaattt tttagcatca tcaagtgtca aatcactaaa ctctacatgg tatccactgt
    11581 aaagttcatg tattgctact acattgtttt cattttgtga atcttttata acactaggtc
    11641 caaatatcaa cataacacct aacataactc ctattattaa aagtaatgtt ctaagtttat
    11701 ttttctttaa atatgctagt gacattttta agtaaatccc catataatcc tcctatttta
    11761 ctataaatta tttatccaac tcatctgtta agtagattgg tggatatttt aaatctttta
    11821 attcttcatt attttttgaa gcttactacc atcttcctca tgataaactt gcagtatcac
    11881 cttatttaca tgtagtgcat aataatcacc attataaaaa tattagtatt tataacctgg
    11941 ataatttttg taaaatgatt tataaagtat cccatcctaa ttctattttg tatacttttc
    12001 ttttgtaata cgctctatat catcacctga aactccttga tatgtcatat atttatctat
    12061 atatttttca gcttcattcc attttctaaa atagttgttg tatataataa atcctattat
    12121 ggctataatg cttataatta aagttaataa aatagctttg tattttctca tagtgaatct
    12181 tcctacttca atttaaattc tttaataagt tttaattcta aagacttagt tttatcatca
    12241 aatttcttaa cttgaatttt tatatctccc aaattatcaa cctctacatt tacttcacct
    12301 ttatcaaata cttcttcaat ttttctaaaa aattctttct cacttatttc tatatgaatc
    12361 aatgatctat atgttataaa tggccattat gaatttatca tcttctgaca gcttttcatt
    12421 ataagaaaaa tctttaacac attgaattga taatatttcc ttcgtatcct tcttttcctt
    12481 agttattgaa taatgtaaaa cttggctagg ttgttctttt gtatactcca agtttcccaa
    12541 agacaaaaca aaacttccat ctgatttatt ttcagctacc ctaagaccac tattttctat
    12601 tgattctgat attttctttg acaagtcttc ttgatactag aaatctgttc aatagtatat
    12661 attttttcat ttgtacctga aaccttattt gtgcatcctg ttattaaacc tagtgttaag
    12721 cttatagcta taaccatagt tattgtaatt tttttatctt catataatcc tccttatata
    12781 ttgaaaactc gttatttctc ataaaaaaaa gacgctatct ttttctatta aaagataaca
    12841 tccctatatg tttagtatca atatatgttc cccaactggt aggttaatcg acccaactat
    12901 caaaattttg atttaatggt atataaatat tcaatataaa tttgtctttt ttatcttcaa
    12961 atgataaatc tcctctatat ttttttatag aagatttgat gctttaaatc cctattccat
    13021 gtacaagttt atctttttta tctgttaata ataaactttt attctttcta attgaattta
    13081 ttttactatt ctcacattta attacgaaat aggactttga gatagttcct tttatttgta
    13141 tatatttatc aatactttta tcatgtatct tatcgcaagc ttctattgca ttatctaagt
    13201 atttgcaaat atattagata catctattgg ttttataaaa ttcactttgg aaaaattgat
    13261 atcacaaatg aatttaattt ttcttttttc acttacatca cttttttcat ttattattat
    13321 atccaatata acattacctg tattgtaaat tgatttaaat tctttaattt aatcttttaa
    13381 actatttaca tacccatcaa ttttcctttt ttatatttga gattttctat acaatatata
    13441 tgattattta tatcatgata aagcttctta actttagcgt gtgattcttg aatacttaga
    13501 taatgagaat actgcatatc taacttattt tttattactt cattttgcaa ttttaactta
    13561 ttttccttaa ttatttttat aaacactata ataatgacta agctactagc tagtattaac
    13621 ctaggtatca caatataccc aactatatat atttcctaat acccaccaaa tcgat
//
LOCUS       DAYAOY010000110        13629 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYAOY010000110 DAYAOY010000000
VERSION     DAYAOY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.110"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            192..605
                     /locus_tag="ACGI6B_003328"
     CDS             192..605
                     /locus_tag="ACGI6B_003328"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9752743.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            796..2013
                     /locus_tag="ACGI6B_003329"
     CDS             796..2013
                     /locus_tag="ACGI6B_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HFG9752744.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            2175..2873
                     /locus_tag="ACGI6B_003330"
     CDS             2175..2873
                     /locus_tag="ACGI6B_003330"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFG9752745.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(3093..3710)
                     /locus_tag="ACGI6B_003331"
     CDS             complement(3093..3710)
                     /locus_tag="ACGI6B_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HFG9752746.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(3837..4859)
                     /gene="argF"
                     /locus_tag="ACGI6B_003332"
     CDS             complement(3837..4859)
                     /gene="argF"
                     /locus_tag="ACGI6B_003332"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFG9752747.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(4968..6155)
                     /locus_tag="ACGI6B_003333"
     CDS             complement(4968..6155)
                     /locus_tag="ACGI6B_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFG9752748.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(6185..7045)
                     /gene="argB"
                     /locus_tag="ACGI6B_003334"
     CDS             complement(6185..7045)
                     /gene="argB"
                     /locus_tag="ACGI6B_003334"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFG9752749.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            complement(7060..8277)
                     /gene="argJ"
                     /locus_tag="ACGI6B_003335"
     CDS             complement(7060..8277)
                     /gene="argJ"
                     /locus_tag="ACGI6B_003335"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFG9752750.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(8318..9352)
                     /gene="argC"
                     /locus_tag="ACGI6B_003336"
     CDS             complement(8318..9352)
                     /gene="argC"
                     /locus_tag="ACGI6B_003336"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFG9752751.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(9606..9819)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10037..10804)
                     /locus_tag="ACGI6B_003337"
     CDS             complement(10037..10804)
                     /locus_tag="ACGI6B_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HFG9752752.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     gene            11103..11576
                     /locus_tag="ACGI6B_003338"
     CDS             11103..11576
                     /locus_tag="ACGI6B_003338"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9752753.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(11945..12502)
                     /locus_tag="ACGI6B_003339"
     CDS             complement(11945..12502)
                     /locus_tag="ACGI6B_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9752754.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            12760..13458
                     /locus_tag="ACGI6B_003340"
     CDS             12760..13458
                     /locus_tag="ACGI6B_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9752755.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         4663 a         2149 c         1506 g         5311 t
ORIGIN      
        1 tctttttata taacttaaac tacattttta tttaaaaaaa tttaatatca acaatataaa
       61 attaagaata agtattatta gtttgacatt agcataaatg cctattagaa taatgttaaa
      121 ctatattctt tctactgatt ttattaaaaa ataccaatat acaaaagatt atacaaggag
      181 aaagttcaaa tatggaatta gaaattacag aaaaaataga aaaacaagat gaagatacta
      241 tttttcaagg attattaaaa tataatcttg ctagacttga agataaaaac ccagtggatt
      301 taggaatata tattcgagat aaagcaggta aaattgttgc aggttttata ggtgttactc
      361 atggtaattg gctttctata aaatacttat gggttagtga gaagcttaga tataaaggta
      421 ctggaagtca acttttatat aaggctgaaa aaattgctaa agaacgaggt tgtaaatatg
      481 tttttttaga tacattcagt tttcaagcac ctaaatttta tgagaaattt ggataccata
      541 aagtattttc acttgaaaat tatcctctaa ctggaaagag atattattat agaaaaatac
      601 tataataata aaaatcatat ttcatatttt taaattaaaa cttctgctct tcgtctacag
      661 actttatgtt tttatatcat tattaatact aaaatagtat tttactatac atacaaaatt
      721 atttgaaagt tcaaaaatta taagtataaa agctaatctt agcttaaata aaaattatat
      781 ttttaggagg taaatatgtt aggaaaaaaa tgtatggatt atctacaaac attaggaaaa
      841 ataagttcaa caacaaatgg tcttacaaga cttatcttga ctcaagaaca taaaaaatct
      901 attgatttaa ttagttcatg gatggaaggt ttaaatcttg atattgaaat tgatgacatt
      961 ggaaacgtta taggtacata taaatcaagc tttccaaatg ctcccacatt agtagttgca
     1021 tctcatcaag atagtgtaaa gtgtggaggt atatttgatg gtatgttagg tataatagtg
     1081 cctctagttg gtcttgaaga agcaaaacac aataataggt cttatccttt caatattaag
     1141 ctaattgctt tcgctgaaga ggaaggaact agatttgaaa cttctttaat gggaagtaaa
     1201 gtttttgctg gtacttttaa agaggaactt cttaaatctg ttgatgaaaa tggaattaca
     1261 cttgaagaag ctgtaacaaa gtttggattt aatacaaaaa acttaacaaa tttgcatcct
     1321 agaaaagatg tagatgcata tttagaattt catattgagc agggtcctgt tttagaaaat
     1381 gaatctcttc ctgctggaat tgtatcaagt attacaggat ttaaaagttt taagatatca
     1441 gtaaatggta agtctggaca tgctggtaca cttccaatga atatgagact tgatgctgga
     1501 tgttgtgcct gtgaatgtgt attagctatc gaaaaagtag ctaaaacgac tactgatttg
     1561 gttgctactg taggaaagat gaatttctat ccatcttcat caaatgtggt acctgaaaga
     1621 gctgaattta cactcgatgt acgctcttgt tcacaagaaa ttcttgataa ttctgtagaa
     1681 aagatattta atgaaatatc acatatatgt gaaaatagaa aacttaatta tacaagtgaa
     1741 cttgcttttg aaaatgttcc tgtaccatgt tctaataaaa taacaaaaat catagaaaaa
     1801 agttttatag atttaaatct taatcctttt tatatttata gtggtgctgg ccatgatgct
     1861 caggaaatgg ataatataac tgatattggt atggtattta taagatgcgc tgggggcgta
     1921 agtcataatc ctaatgaaag tgtctctgta gatgacttgg atactgctgt aaaaatattt
     1981 ttaaaaatac tagataatct tgatttaaaa taattcttat ataagaaagt atattaaaat
     2041 atataatcta aaaaatcact ttatatttat attatataag tgtacaatat aatctattat
     2101 aaaatataat tttatgcttt tatagatttt tattttatac ataaatatat ttatatataa
     2161 aggagataat ttttatgaat gaaaaattaa ttggtatttt ggctggaatg ggtccaaggt
     2221 caacagcccc ttttgttgat ttaattattg atgagtgtca atctcaatat ggagcaaaat
     2281 atgatgatga atttccaaag atgatgatat attcattacc taccccattt tatattgacc
     2341 gtccaataaa tcatgacctt atgaaagaaa ctataattga tggattacaa aagcttgaat
     2401 caactggtgt aaattttatt gcaatgccat gcaattcagc acatatatac tttaaagaat
     2461 taaaagagtc tattaatata ccaatattaa atattgtaga agaaacagtt aaaaaattac
     2521 ctactatctc acaaaaagta actctttttt cgactagttc aacttttgag tctacaattt
     2581 accagaatgg tataatacat aatggacatg aatttatatt taaagatgaa tggcaaataa
     2641 aattaaacaa tcttattcaa aatataaaaa tggataaaga aaatccacac aatattgata
     2701 tttggaataa acttattgaa gatgttaaaa atgaatctat agaaaatata gtaattgctt
     2761 gtactgattt aaatgtagtt ttagaaaaat catatccttc aataaatatt gttgattcat
     2821 caaaatgctt ggctgaatca gttgtcaata aatatctaaa attagttaaa taaatttttc
     2881 atacactata ttataaaaaa tatatgttat aagtttattt taaatagtat agacttataa
     2941 catatatcat agtttcttga caatatagaa attaaccatg tatttcatta tattaataaa
     3001 aaggatacca aaaatggtat ccttcataaa tatttatatg tttatttata aaaataatca
     3061 tatatttatt actttactac acctaaacaa tattattcaa gtgtattcat agagttagaa
     3121 aattcttgtc ctgctgtaac aagattagat aacaatacat cagctactgc atcatttact
     3181 gaacttatta tccatttatt atccttttta gaacattcaa actctacatt ttttgtaaca
     3241 taagtctctt taacgctttt ttgtttttct ttcataattg atactaacat ttcagttgtt
     3301 ttctcatcag ttaagtcttg tcctgaaaaa ctcataccaa tcatctttgt aaatgcctca
     3361 gctactattt cttgaagtaa tggtgttgaa tctataaatt tacattctac tttgattgta
     3421 gccttatcac ctttaacctc gctgtctttt attgtatatg tgattttaga tgcattctgt
     3481 tttagataat ctaaaacata ttgggcattt gggtcttttg tatctaattc ctttaatata
     3541 tccttatatt tttcgtcatt attttccatt accttattca tgccttcaaa gtcaaacttt
     3601 ttcgcactgt taaaaaaact atcaattgta tcttctggtt ttgcacttga acatgctgtt
     3661 aaagatattg ctaataaaaa agttgttaaa gctaatatga tttttttcat atctcaaatc
     3721 ctccctctta atttgttgat acataagtta ttttaacata taatgagagt ttttgaataa
     3781 tatatttaaa atattttata aattttcatt aaagtaaagt ctatattaaa acatatctat
     3841 attaaattat tgttcatatt tacatcagtc atagtagaaa ccataacagc ttttattgta
     3901 tgcattctat tttcagcttc ttcaaatacc tttgaatatt ttgaattaaa tacctcgttt
     3961 gtaacctcta attctgataa tccgaacttt tcataaatct cctgagcaac cttagtttct
     4021 aaatcatgaa atgctggaag acagtgtaaa aatataacat tttcattttc agttttcttt
     4081 atcatatcca tatttacttg ataatcttta agttgattta ttcttgcttc atattgctcc
     4141 tcttctccca tagaaaccca tacatctgta tatataacat ctgcaccttt aacatcatca
     4201 atattttcag tcaacattat atctcctttg gatttcttgg ctatttcctt catttcctta
     4261 accaattctt catttggcca aagttcctta ggtgctagtg ctacgaaatg tattcccatt
     4321 ttagcacatc ctatcatcag tgagttcccc atattatttc tagcatcacc aacatataca
     4381 aatttaactt tattcaaatc tttatctaca ttttcaatta ctgttaaaaa atctgccagt
     4441 atttgtgttg gatgatattc atctgtaagt ccattccaaa caggtacacc agaatatttt
     4501 gctagacttt caactgtttc ttgtttaaag cctctaaatt ctattccatc atagaatctt
     4561 cctagaactt ttgctgtatc tgatacagac tcttttttac ccatatgaca atcatttgga
     4621 cctagataag ttacatgtgc tccttcatca tgagctgcta cttcaaatga acatctagtt
     4681 cttgttgatg ttttctcaaa taataaagct atattttttc cttgtagcaa atcgcctttt
     4741 ataccaactt ttttcttagt ttttaaataa cttgataaag ttaataaata tctaatctct
     4801 tcttttgtaa aatcctttaa tgtcaaaaaa ctcttacctt ttaaacttat attattcata
     4861 ttaattcgcc ccttcttata aactttttaa attcaattaa acttgttagc actttacttt
     4921 tttatttact tctaattttt atctttaatc aactattttt tgaatattta gttaatttct
     4981 agtaatatat cttttaggat ttcaagagcc tcatctattt ccttataact gattgttaat
     5041 ggtggtaaaa atctcaatac attttttcca gcagtcaaaa taagcaatcc tttttttcta
     5101 gctttctctt ctataatact agattcaata ttagtttcaa ttcctatcat tagaccaata
     5161 ccttttgtct ttaatataac tttgttatta aaactttcaa tagattttct tatataattt
     5221 cctttttcta atacttcatt ataaaagcca tctttagata tctcatctaa tacaactgaa
     5281 gctgccacca tagctaataa attacctcca aaagttgtac cttggtctcc ttttcctagt
     5341 acttttgaaa gttttttatt taccaatact cctcctactg gtattcctgc tcccaaccct
     5401 tttgctacgg taactatatc tggttttaca tcaaaatatt catagccaaa aagtttacca
     5461 gttcttccta taccgcattg gacttcatca aaaatcacta caatatcctt ttcttgacat
     5521 atttttacta tttccaaaac ataatctttt tcaagaacat taactccacc ttcgccttga
     5581 atcgcttcaa gaattattgc acaaactgtg gaatcaagat tattttttaa atcttcaata
     5641 ttatttcttt ctatatattt aaaaccttcg ggaagaggat aaaaatactt atggtatttg
     5701 tccataccag ttgccattaa agacatcatt gttctaccat gaaaagagtc ttttaaactt
     5761 atgatagttc ctctaccatt accatatttg tcactactat actttcttgc tattttgaat
     5821 gctgtttcat ttgcttctgt tccagaatta caaaaatata ctttagccat tccacttgaa
     5881 ttaattattt tctctcctga atacaacact ggctcattaa aatataaatt tgaagtatgt
     5941 aataatttat ctacttgctc ttttagagca ttctttaaat tactattact atacccaaga
     6001 gaagatactc catatccact tgtaaaatct atatatttat tgccattagt atcaaaaaaa
     6061 caacttcctt caccataatc tatgacaaaa ttgggttggt tatatgtatc tataaaatat
     6121 tcattccatt ttgaaattgt attgtcgtta ttcattctta ttctcccctc ttaaacttat
     6181 aattttattc agtttctttt cttattaatg tacctattcc actatcagta aatagctctg
     6241 ttattataga atgtggaact cttccatcca aaatatgtac cctatttact ccgttattta
     6301 aggcatctac gcatccctct attttaggta tcatacctcc agtaattata ccttcttcaa
     6361 ataatttatc aacatcctct aatataactt ctgttattaa tgatttttct tcatctggct
     6421 ctcttaacaa accagggaca tctgtaagaa gtatcaattt atctgcattt aactctttag
     6481 ctatagcaga agctgctgtg tcaccattta tattataagt ctctccatca tctccaacac
     6541 ctatagttgc aattactgaa atataacctg actttagaca agattctata agctcaacat
     6601 ttactttttt gatttctcct acaaatccaa gttcataatt tttatatggg tcacataaaa
     6661 tcatattatt atctattcca caaagcccta ctgcttttcc accttttgta tgtattttat
     6721 ttactaagtc tttattaacc tttccagcaa gtaccatttt tacaatttcc atggtctctt
     6781 catctgtata tctaagacca tttacaaatt tactttctat atccactttt gctagcatct
     6841 tattgatttc tgcccctcca ccatgtacta aaactatatt gattccaaca taactcatta
     6901 acacaaggtc ttccataaca gattctttta gaccatcttt tttcattgca cttccaccat
     6961 acttaactac aatagtcttt ccctgatgtt tttctatata tggtaaagct tctataagtg
     7021 tatttgcttt ttctatatta atcattaaag tatatcctcc taatttctat aacttccatt
     7081 tattcttaca taatcatagc ttaaatcaca tccccatgca tttcctctgg cattgcccat
     7141 atttaaatct actaatatac ttatttcgtc atcttcaagg attttttttg ctttttcctc
     7201 attaaaatct aatccatttc catttttaca tacttcaata tatcctttca tactctcaaa
     7261 aataatatca actttttcca tatctatttc ttcacctgca taacctaaag cacacagtat
     7321 tctaccccaa tttgcgtcag ctccaaatac agctgtcttt actaatgaag aagatataac
     7381 tgtttttgct aattttactg catgttcttc acttaatgct ccatttatat agcattcaat
     7441 tagttttgta catccttctc catcttttgc aacaagttta gcaagttcta tacagacata
     7501 tgtaagtgca ctcaaaaata cttgataatg ttcatctttt ttgtttattc tatcattttt
     7561 tgcttttcca tttgctagaa tcattaccat atcattagta cttgtatctc catctacact
     7621 aactctatta aaacttttgt caacagcaat ttttaaagct tcttttagca aaactccatc
     7681 aatatttgca tctgtagtta taaatcctaa cgttgttgcc atattaggat gtatcatgcc
     7741 agaacctttt gccatacctc caatagttat ttttttatct ccatacatta ttgctactgc
     7801 tatttctttt tttataatat cagttgtcat aatagcttct cttgctccaa tatgaccttg
     7861 tttacttaga ttattcacta attcatccat attttcttct attaaatcta tatttaatgg
     7921 ctttcctata acacctgtag atgcaaccaa aacatcactt tctttaatat ttaatttatc
     7981 actggtgaat ttagccattt tataagcatt tataagtcct tcttttccat taaaggtatt
     8041 agcattagca ctatttacta tgatagcttg tgcttttcca tttttaatgt gttcctgtgt
     8101 aacataaaca ggattgccct ttactttatt ttgagttact actgctgctg catcacataa
     8161 aacatctgaa taaaccaaag ctaaatctct tttttcttta ttttttctca agccacagtg
     8221 aatccctgaa gcaaaaaatc cttctgaaac tgtaactcca ccctttataa tctccatatt
     8281 tatcctccta attatatgag tttagtattt tttatttcta aaatgatggt gcaatatttt
     8341 ttaaacctgt attttcttca ataccaaata ttatattcat gttttgtata gcttgacctg
     8401 ctgctccctt aatcatatta tctatagctg aacagattat caatgtatct ccattttcat
     8461 gtaatgatat tacacattta tttgagaatc taacattctt taaagaagct accttatcta
     8521 atggaagaat ttctataaat tctttaaatt cataataatc agttaatttc ctatgtatat
     8581 catttataga aatattatct tcttttgtac aatatatagt tgataagatt cccctattta
     8641 ctgggattaa gtttggtgta aaaactacac ttactttttc tttgcaggat tcacttaaat
     8701 tttgttctat ttctggagta tgtctgtgct tccctatttt atatgcagtg atattttcat
     8761 taacctccgt aaagtgacta ctaatcgata attctctacc agcacctgta agacctgatt
     8821 tagaatcaat tattatatta ttatttttta tcaatttaga acttagtaat ggcatcaatg
     8881 gtaaagaaat actagttgga taacatccag gatttgcaat tatatctgca tcttttatat
     8941 cttctttgta tatttcactt aatccatata cagctttttt atgtaagatt ttatctataa
     9001 aagatactcc ataccattta ctatatactt cttcatcttt tattctaaag tcagcaccta
     9061 aatctataac tttctttttc gatttaattg cctttattac aaacttttca ctaactccat
     9121 gtggaagggc tgtaaataca acatcacaca tatctataac tttttcattt tctatacata
     9181 tcatgttatt tttatatcct atacttggat atatatcaac tatatctttt ccaacatacg
     9241 aatttgaacc tatcgctgtc acttcgactt tatcatgatt cattaataat ctaatcaact
     9301 ctgccccgac atatccagtt gcaccaataa taccaacttt tattttattc attttgttcc
     9361 ctccattttt atattcaaat agtcttaaaa tcctatcttt ccactttact atatgataat
     9421 catttgataa tacgtagatt aaaatactct aattaaaaca aaaaacccct tagcattata
     9481 aatactaaga ggcgattttc cgcggtacca ctcttattgg taaaattaca ataatcatta
     9541 aaactcaaat taaaaagtat ctacaaaatg tacatttaaa attaaaaaag cctcttggtc
     9601 aaagaccaag agacgaatat acacccgcgt taccactcta attgataaga taatcttacc
     9661 cactctaatc tcttaaggcg agattcacca attaccatac tattacttct ggtaatctcc
     9721 tccaaagctc tattcacaat aaaattcgtt actaggcttc caccattccc agctctctat
     9781 caactagctt tatgctactc tctttttcac aggatttcaa tttattttct tattgtttta
     9841 tatgatagtt tattttttta actttgtcaa tataattttt aaatttttgg aaatattttc
     9901 tatatggtta atttccagca ttttgtatct aaattgcgat ataattttta tatttcattc
     9961 gtcaatttaa aaaacatcca aaatatatta aattataaaa taatatattt tcaaaataca
    10021 atatttacat ctctttttag ttaaattttt taatgttggt atctaaaaat ttctcaatct
    10081 ttttctcaga aagtatctcg ttggttacaa attcaccatt aaaaaaaaga ctgtatgtag
    10141 taaatggtgc aggagcattt tgtgcttctt ttgctgtttc gaattttata agatttacat
    10201 caatacctct agaatttgca atttttttca aaataggagc atatttttcc gtatgtggac
    10261 actgattact gtaatataaa actataccgt tctcatttaa ttttcttttt tttacacaat
    10321 tcttaaaata tggaatagga gcattatcag taaaaggaaa atataatagc tcataatttg
    10381 gttctgatgt atctgctaaa caaaagtcct tatattttag atatttagca tcagataaaa
    10441 aaggcatttt tttactagac gaaagcacaa ctaatccata tttacctttt gattttgcat
    10501 catcaacaca ctcttgtagc aatagattag aatatccttg acccttaaat tttccagata
    10561 tccaaaaaca gttaataaat atataattgt ccgcagtaat aggacaccat gcattttctg
    10621 ctggaatata ttctataaaa gctttacctc ttacatcaaa tcgtttaaag actagacctt
    10681 tatcaaaatt atttttcatc caagcttttt tagaaaaaac acaattttct cctttttttt
    10741 cagaaattga acaacatata tgttcattgt caatattatt tatatttata tcaataatct
    10801 gcatacttat ctccttttct aattgcataa taatttttaa atatattcta tatattttta
    10861 tcttaatatt ttgaatacaa caacatcatg tcatatatat ttttatttta cataatatta
    10921 ttttaagatt atctgaatta ttaaattgta tatataatta aatacattct atggtctaac
    10981 tacactataa tataaattat atattatcat aatattctaa atttataaaa caaattgtat
    11041 tgaattgaat ttatttaaga tatataatta taattaaaat aataataaag gtgttgttta
    11101 taatgttcaa agaatactta ataagagaat taaaagaaga tgatattaaa aatgcattag
    11161 acttaatctg gaacgtattt gaagaatttg aatctccaga ttattgtgat gaaggaatta
    11221 cgacatttca tcaatttata gaatataact ctattattga aaaaatcaaa gaaaaaactc
    11281 tcttcttttg gggttgtttt gactctaaaa aacttattgg agtcttagct attagaaatt
    11341 taaatcatat atgtatgtta tttgtagata aaaactatca taaacatggt gttgcaaaaa
    11401 aattaatgac tacaggcata caatactgta tttgtaataa atttaattta actgaaataa
    11461 ctgtaaattc atcaccttat gctgttgaag tttatcataa attaggattt aaagatactg
    11521 ctgttgagca aattgttgac ggtatacgtt ttacacctat gaaattgtta atataataaa
    11581 ttaaataaaa agcaaagtct cttaaatatt ttatagttac tttgctttta aaccaagatt
    11641 taaaataata ctatcttttt tgttttttaa taatatactt taaaatcctt actaatatga
    11701 actataattt attttttgat atcctttttg attaacatat aaattaattc aattattaaa
    11761 cataaactat gttgtttctc tttatatctc ttctttgtaa acaatattgg gcatttgaaa
    11821 ataaatattc ttaaaatata ctgttctcct gttgattttc ctttggtaca aatgataaaa
    11881 atttaattta catgccccat aatgaagtgt tcaataaata agttaaaata atttatttac
    11941 ctccttaatc atatttttta tgaataccta ataacacagt atcaatagaa tcggctaaac
    12001 cttttatagc ttcttcttta ctcaaaagtg atttcccaag ttgcaaagtc gaataaccat
    12061 gaagtacact cattattaag tttaatactt cacctgtatt ctttgtattt agattacaag
    12121 aaagaataag tttttctaat aaggatttat attcatcaaa gatttctaat gtttcattat
    12181 ttccatgcca agtagcccac tgaattgttt cataaacacc aggattagaa attataaaat
    12241 taaagtatgc tatacttata cactttatag ctgtatctcc aaaatttcca atagtcgcct
    12301 ttatcatttg actattcatc tctctcattc ctttatgagc tacttcaagt aataaatcat
    12361 ctagactttc tatatgattg tacaaagatg gtgtacgtat tcctagcttt tctgcaataa
    12421 cttttaaaga aactttgtta agcccatctt tattaactat atctgatgta gcttgtataa
    12481 ctatttctct actaacacgc atgattttcc ctctttcatt taaaattaat tactttatta
    12541 gtttagctaa tctaattagt ttcgtcaatt attgtaattt ataaaattct ttatactatt
    12601 ataaaatctt gacagtttaa ttataattta atataatact aattgaatta gttttaatac
    12661 taatataatt agtattatat taaattctat attacaacat tattttatag tttgattaat
    12721 ttaacaatca ctaatatatc aaactgaaag gagattttta tggaaaaaaa atatttcgaa
    12781 attcatggag ttccttcaat cctttgggga aatccttcaa gtaaattata tatttacata
    12841 catggacaat gtggcaataa agaggaggct gaagcttttg ctaatattgc agttccacat
    12901 gagtgtcaag ttttaagtgt tgatttgcct gaacatggag aacgtaaaag agaaaaaaat
    12961 tcatttaacc catggcatgt tgttccagaa ttagtatcta ttatggaata tgcaaagtgc
    13021 cactggaatc aaatttcgtt atatgcaaat agcattggag catggtttag tatgttaagc
    13081 tttgaaaaag agaacttaag agagtgtatt tttgtatctc caattctaga tatgcaacag
    13141 ctcatttcaa atatgatgct ttgggcaaat gtatcagaag aacagcttaa acatgaacta
    13201 attatcccaa cttcatttgg gcatactctt tcatgggaat atctacaata tgtaaaacaa
    13261 catcctatca ctcaatggaa tgtaccaaca aaaatattat atggtaaaca tgatgaactt
    13321 actgaaatcg gtgttgtaga gaaatttgta gaaaagttta attgcaacct tactattata
    13381 gaaaatggca aacattggtt taacacacca caagaattaa attcattata tgaatggtta
    13441 aatactttag ttaaataaaa aataattata ttattaatat gattaaatta tttataaatg
    13501 ttatttaatt aattttattt tagcattatt aaaataacta cataaagaat aaattagtta
    13561 ttattaaaga aattaaaagt atatagagta ttaataattt tttaataatt agttattata
    13621 gaaaaaaga
//
LOCUS       DAYAOY010000111        13444 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYAOY010000111 DAYAOY010000000
VERSION     DAYAOY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.111"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            730..1392
                     /locus_tag="ACGI6B_003341"
     CDS             730..1392
                     /locus_tag="ACGI6B_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752756.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1401..3908
                     /locus_tag="ACGI6B_003342"
     CDS             1401..3908
                     /locus_tag="ACGI6B_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9752757.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4239..4583
                     /locus_tag="ACGI6B_003343"
     CDS             4239..4583
                     /locus_tag="ACGI6B_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752758.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            4821..5507
                     /locus_tag="ACGI6B_003344"
     CDS             4821..5507
                     /locus_tag="ACGI6B_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752759.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            5518..6759
                     /locus_tag="ACGI6B_003345"
     CDS             5518..6759
                     /locus_tag="ACGI6B_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9752760.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            6830..7513
                     /locus_tag="ACGI6B_003346"
     CDS             6830..7513
                     /locus_tag="ACGI6B_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752761.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            7510..9843
                     /locus_tag="ACGI6B_003347"
     CDS             7510..9843
                     /locus_tag="ACGI6B_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9752762.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(9951..10513)
                     /locus_tag="ACGI6B_003348"
                     /pseudo
     CDS             complement(9951..10513)
                     /locus_tag="ACGI6B_003348"
                     /inference="COORDINATES: protein motif:HMM:NF037635.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10627..11976)
                     /locus_tag="ACGI6B_003349"
     CDS             complement(10627..11976)
                     /locus_tag="ACGI6B_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9752763.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(12013..13233)
                     /locus_tag="ACGI6B_003350"
     CDS             complement(12013..13233)
                     /locus_tag="ACGI6B_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9752764.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         5311 a         1521 c         2035 g         4577 t
ORIGIN      
        1 tttgtatcat atgaaacctt tatagattat gaaagcaata atatagtgta tgatatataa
       61 aataagttat aaaagtattt ttttagatat tcaaaaagac attgtaagtt atattaataa
      121 tatgggtatc aacgaatttt ggatataatg agtttttcaa cttaaagtta tgtaataatg
      181 aatgtaaaga atatttaatg aaatcatttt gttggttatg tctagctgaa tacaataatc
      241 ttttagataa agattttata tttgaagata ggtgtgaaaa aattaagaag atattaacat
      301 cagcagttta gaggcaaagc atgtaaagta aagtctgggt aactaaaagt agtaatttag
      361 attgtaacta ttgttataaa tatgaaaatg aggaaacagg aaatataagt taggatgtag
      421 aaaataggtt actaaataaa aagcttaatt atataatcaa agaaataggt atatactgta
      481 caatttcatg gtgaagaact tttcctaaat aaaaaggttg gtaaaagaga tataagaaat
      541 aaaattcaat tataagatat ggtatgataa ctaaaggcac tatatggagt gaataggcgg
      601 aagaattttt tgaaaattat aaaaatagtt tctgttgcta catatcaata agtatagata
      661 gagattgcaa aatccatagc aaaaatcgaa tttataagtg taaataacat gtaagacaag
      721 gagtaaatta tgagtatttt agagataaaa aacctaaata aaatatatgg taaatcggaa
      781 acagaggtga aagcgttaag tcaaataaat ctaaaaataa atagtggaga atttgttgtg
      841 atagtaggta aaagtggaag tgggaaaagt actttactac acataatggc aggacttgaa
      901 actccaacaa atggtgatgt gattattgat aatataaata tatcatctct tgatgaaaag
      961 aaaagatctg cattacgtaa agaaaagttg ggattaatat ttcaatcata taatttgatt
     1021 ccagttttaa ctgttgaaga aaatataaaa ctaccaacta taaatataaa aaataaagat
     1081 gaagcttata ttaatgaatt gatggagtta ctaggaataa aagatagaag aatgtatttg
     1141 ccaaatcaat tatcaggtgg gcaacaacaa agagtagcaa tagcaagggc tcttgtaaat
     1201 aaaccatcaa ttgtatttgc tgatgaacct actggaaatt tagacacaaa gacagagtca
     1261 gatgtattaa gtttattaaa agaatctcaa aaaaaatata atcagacaat aataatgata
     1321 acacataata tagatataac aaaatatgct gatagagtta tagaaataga agatggattt
     1381 ataagagagt aggtagattt atgaaactta attatattgt aaaaaaatat ataatgggaa
     1441 ataagaaaaa tacattatta ataattgtaa gtattgtaat ttcaacagcg ttgtttttaa
     1501 ttatgaatat aataagtgaa gatgcgacaa acttaatgat aaatcaagct aaaaatgaat
     1561 ttacattaaa acatgcacaa tatatcaatc caaaagacga agaaataaaa tatatagaaa
     1621 ataacacaag tatagataaa gttggaaaat caatgctttt aggaattagt gatattggta
     1681 aaggtcaaac actacaaatt ctatctcagg ataaagttgc tgaagaacta aatgatattt
     1741 acacactaga aaaaggtaaa ttaccagtag ctgaaaatga aatcgctata gattcatggt
     1801 atattaaaca aaaaaagata aaaaacccta taggaaaaag gataacatta gattatagaa
     1861 gacaagggat agatcaagaa gatttatata ctggggaaaa agaatttaaa ataactggta
     1921 tcttaaatag taatcctatt ttaaaagctc aaggaacatc aattggcctt ataagtaacg
     1981 attgtgctat caagaatatt cctactaaaa ataaatatga tcaagttatg tttactttta
     2041 aaaaggaaaa aaatatacag agacaatcac aaaaacttat taaaaatgga aatttgaatg
     2101 agaataacat ttattttaat aatgaattat taatagctat atctgatagt atgaatttaa
     2161 aaatacctta tataatagtt aatatagtac tagcactagc aactatatta ttaatttata
     2221 atatttttta tatattagta tcaaatagaa taaaggattt tgggatattt agggctttag
     2281 ggtttaatcc aaatgatatt tttaaaataa tgattttaga agtatctata tactctatta
     2341 taagtatttc tataggtcta attttaggtg ggataatagc tagtttatca agggagtatg
     2401 ttataggagt aatatacaat gtcaactata taaattctat aaaaaaccag aactatataa
     2461 acacatacat aatttctata ttattaagtt taggaactat aataatatca gtttcaaaac
     2521 ctcttatgtt atcagttaaa actgatccaa tgatttgtat aagaagaaat aatgaaaaag
     2581 tggatataaa acaaaattcc tttataaata aatttatgat aaaacttttc aaagactatg
     2641 gtaacatagc atcaaaaaat atacaaagaa ataagaaacg tacaaattta tctatagcat
     2701 caatggtaat tatatttttc ttaatggcaa caatatacac aaagtctact agtaactttt
     2761 taagtgatgg tgtattaaaa tattggatac aaggagatta tttattacat aatattgata
     2821 taagtactat acgttcaaat aataaaggtt atgataaaaa tgtactacaa gagataaagg
     2881 caattgatgg tgtaacaaaa gtaaactcat ataggcataa gtggtttaat ataaaaattg
     2941 atgataaaag tataaataag aactctgatt attggaaaaa aaataaagat aatattgaag
     3001 ctagatcaga agtaaaagat ggaattaagg tatataataa ttcttttgaa tttttaggaa
     3061 tagaagatac agatatacta gaggatttct tgataaatgg aaaggaaaat atagataaat
     3121 ttagtaaaca accatatctg tatataacaa agaaatccag tgattcgctt aatataaaaa
     3181 aaggagatag aataaaagta tactttgata ttatagattc aaaaacaaat aattttacca
     3241 aaacaatatc tcaagaattt atagtatgtg gaattataac tacattacca gtaacctcac
     3301 aagtgggagc tgaatttggc ggagtaatat caacaaatca atttaataag tttacaggaa
     3361 tatcatctta tgaaagattt gacatatgga cgagtaaact agccaatgat aaatatgttg
     3421 aaagtgagtt aaacaaaata acagaaaaaa caaataaagg aattttaatt ccatacaaag
     3481 ctgaaacagc tgaatatgaa aaaattgata atcaaaaaac actaattatg gtattagtaa
     3541 ctggaattat agttatatta tctttattta attgttgcaa tactatagtt actagtatta
     3601 atagcagaag tagggaattt gcactattta gaggaatagg aatatcaaaa catgaaataa
     3661 ataaaattgt taaattggaa agttatatat atattatagt aagcttttgt atatctataa
     3721 tacctatttt aattgtaaga agtataataa taaaaccttt tgaaagtatc aatttaataa
     3781 attggaaatt tataggggct attatactaa tagtatttat tttagtatct ataatcatga
     3841 taactacact aaaaacattg aataaaattc aaaatgaaaa ttttatggaa gaaataaaga
     3901 ctttatagtg aacagcctat aaattaaaga ggttttatat gaatttaatt gcaattatcg
     3961 ataatggtgt agatattgat tttaaataat ttagaaataa aaataaaata agttatccaa
     4021 gttatatgta tgatgtaata ggggtacaat gtatagcaaa tataaatatt ggcttaataa
     4081 aactctactc ttaagtctac tgtcgtttta acacaagact aataataagt gtaacctaaa
     4141 aattattgat aatacaataa ccttgtttat gataaataac aaatattaag tgttgtaaag
     4201 atagtaagtt taaaaaaata tcaaagattt caatcacaat gtattgttta tctgtagtgt
     4261 tagctattta tggtatatat gtatttataa attgtactga atatatatat actatgctat
     4321 ctacaaaaca tttaacagtc aaaggaaatg aatataccat tataaattat tatatgagta
     4381 actttagtca gtatataata tttgctattt tacttgcatc aatgggattt gtaatacaaa
     4441 agatagtgca tatgcatttc agtgagatag aacttagtgg taactattca acaactgtta
     4501 gaaatataag tgatatctct ttagataatg ataatgaaat gtttaataga ggcaatattg
     4561 aaaatagtat tttaaaaaaa taaataatat aaatatataa aatgtgattg aataaagctt
     4621 agaatactta gtgtatttta agcttttttt attattaaga tttcaaagta ttaaattaac
     4681 tatataaaat catataaaaa taattttaaa atatttaaat cattgatttc aaggtattgt
     4741 atttttaaaa gagtaaaaat ataataagtt aggtatataa tatatataaa gtaaaaaata
     4801 aaaaaatgga agtgattgag atgagtagta tattgttggt tgaagatgat ttaagtttga
     4861 tatatggatt agaatattct atacaaaaga atggatttag tgttgatgta gcaagaacag
     4921 tagaagaagc attgcaaatt tataaagaaa aaaattatga tttactgctt ttggatgtat
     4981 ccttacctga tggtgatggt tttgatattt gtaaaagggt aagagaagct tctaatgtac
     5041 caatcatatt tttaactgct tctgatgaag aagtaaatgt agtgatggga cttgatatgg
     5101 gtggagatga ttacataaca aaacctttta aattaaatga gttaatttca agaataaaag
     5161 ctttacttag acgttataat tttacaagtg aaaatgtaac tgaactaaaa tcaaataata
     5221 ttactgttaa attacttgaa aatcgtgttt ttaaaaatga aattgagctt gaacttacta
     5281 cagcagaata taaattatta tgcttactta tgaaaaataa aaatattgta cttacaagaa
     5341 aaaatatatt ggataagtta tgggatggaa atggtagttt tgtagatgat aatactctgt
     5401 cagtatatgt aagaaggtta agaaataaga ttgaagataa tcctgaaaat ccaaaaaatc
     5461 tattaacagt tagaagaatg ggttacaaat ggaatgttat aaagtgaggg tgttaatatg
     5521 aaggtatttt ctaataaaga cataaaaata ttttttttgg tgattatatc cgttttattg
     5581 atgtttatag tagtaggtca aatagtagtt ataaatataa ctaatgatta taaaagtgca
     5641 ctgttagaac atgattataa tatagctgga tacttaaata gtatgggtgt tgataaatca
     5701 aaaataccag cagtatttac tactaatgaa gataaatata ctttaaaagg aaaagaaatc
     5761 ttaaatacag ctagatatga ctttggtaca gatgatagtt ttatgccaag tgtgaaattg
     5821 tttcaaggta aatatataaa aactatgttt ttgtatttat ttgtgttctt tactatgatt
     5881 atttttatat tgtatgtgta tttttttagg caacagaaca agatagaaga agctagttat
     5941 aaaattgatt catttatgaa tggaaatata aatgtacggt taaatactta tgaagaaggt
     6001 agtttatcaa agttatttgg ttcaatagat gctatgtcaa catctctaaa cacacatata
     6061 ttgaaagaaa agcagaataa agaatttctt aaaaatacaa tttcagatat atcacatcaa
     6121 ctaaaaactc cacttacagc attgatgatg tataatcaaa tattgcagga agagagtcat
     6181 aatagtgaaa ttgttgagaa ttttgtgcag aagagtcaaa atgaattaga gcgtatagaa
     6241 tcattgatac aaaacttgtt aaagattaca aagatagatt caaagacaat aattcttaac
     6301 aaagctagtg ttaacgtaaa aaagttgatt actaaaattt tatatagttt tgaaactaga
     6361 gcagaaaaag aaggaaagag tatctctata agaggtctag aaaatacaaa cttgttttgc
     6421 gattatgaat ggatatcaga agctttaagc aaccttataa aaaatgcatt agataataca
     6481 aaagagaacg ataaaataat gatagaatgg gttgaaacac ctataacaac tattatttca
     6541 gtgagtgata ccggaaatgg aattcatatt gaagatatac atcatatatt taaaagattt
     6601 tataggagta agttttcaaa aagtaatcaa ggccttggat tagggttgcc actagttaaa
     6661 tctatcatag aacagcacaa tggaaccata actgtagaaa gtaatttttt acaaggaagt
     6721 acatttactc taagtttttt aaagcttact aatatgtaag ataggataca tcttacagta
     6781 aggtgtatga attattctta tattaatcat cagaaaggag atagagcaaa tggatattct
     6841 tgtggttaat aatttatgta aaacatatgg aaatggatat acaaaagtag aggcattaaa
     6901 agatgtatca tttacagttt caaagggaga gtttgttact gtagttggag aatctggctc
     6961 aggtaagagt acattattaa atcttttagg aggtcttgat actccaacct caggaaaaat
     7021 atttgttgat ggtaaagaaa tatgttcaat gaaagaagaa aaacagacta tttttcgacg
     7081 tcgtaatata ggttttatct ttcaatcata taatcttata cccgaattaa ctgtggaaca
     7141 aaatataata tttcctttac tactagatta caaaaaacca gacactattt atctagatga
     7201 agtattgaca attttaggtc ttaaagatag aaaaagtcat cttccaaatc aattatctgg
     7261 tggtcagcaa caaagggttg caattggaag agcattgatt acaaagccat caattatatt
     7321 ggcagatgag ccaacaggaa atcttgacag taaaaatagc agtgatgtaa tttcactttt
     7381 aaaagtgaca gcagaaaaat ataaccaaac tattattatg ataactcata atacaaatat
     7441 agcttctaca gcagatagga tactacaggt ttctgatgga atgcttaaag acctaggggt
     7501 gaatagagaa tgagaagcta tttagaacta gtaccacaat atgcaaaggt acataagaaa
     7561 aaaaatagaa ctacaatttt atgtattgtc attgcagtat gtttagttac tgctattttt
     7621 ggactagcag acatggctat acaatgtcaa aaagtacaat ttataaaaga tgatggaaat
     7681 tggcacatag tacttaaaaa tcttaatgaa gaagctatat cagaaataag tagtagagta
     7741 gatatagctc actatggtag aggacaagtc attgataatg gagagtttaa atcaaaggag
     7801 attttgatac aaggtttaga tgaagattta gcaaaacaat taggtctaga aataataaaa
     7861 ggtcgttatc ctgttgtgga gaatgagact ttaatagatg tagatgcagt taaaagcttt
     7921 aatctaaaag taggagattt tatcaaaata aaattagcag atggtagaaa taaaaattat
     7981 aagatagtag gtgaatattc atcattatca agattaagag cctcagataa gcatggatta
     8041 atgctttcaa tattaggtac acaagctatc aaaaaatcag aaacatatga ttattattat
     8101 attacattta aagacaaggt taatattaga aaagctactt ctgatattaa gaataccttc
     8161 aatttaagtg ataaacaaat cggtaaaaat gaaaaattac tttctattat aggtcaaggt
     8221 gactctgatt attctaaacc actttactta acagcaggag gactttttat acttgtgtta
     8281 atagcaagta ttatcatgat atataatagc tttaatatgg gtgtaataga gagaataaag
     8341 ttttttggac tattacgttg cttgggagca tcaaaactcc aagtcaaaaa atttgttgtt
     8401 ttagaaagtt taattctaag tgtaaaggct attccactag gattgttatt aggatgtatt
     8461 gtaaccatta tatcctctat atttctcaaa tatgtgaata cagagttatt tggttcgatg
     8521 ccaattttaa aggttagctt tattggtatt gtgtttggaa taatagtagg atttttaaca
     8581 gtaatattat cagctattgt tccagcaaaa aaagcttctg gggtgtctcc actaagtgct
     8641 atacgtggta atctaatgtt caaagaaatt ttaactagtg gaaaagcaag taaaattgct
     8701 aacgcaggaa gttctaaaat tgatgtttct atgggaataa atcatgcctt ttcaagaaaa
     8761 aaatctttta tacttatgac ttcttcattt gctattagta ttgtattgtt tttaggtttt
     8821 agtgtattta tagactttat gtatcaggca cttaaaccta taaaaccaga agctgctcat
     8881 atatctattg ttgacgctga ctttaagtct gttcttacta aagacgatat taatgaaata
     8941 agtaatatca aaggaataaa aaaagtatac agtagaatga tgacggatgt agaggctaca
     9001 tacaataata aggaggcaac atctcagcta atttcttatg aaaaaaatca atttgactgg
     9061 gccaaaaagt atctagtaaa aggggaaatc aatgaagata aattagagtc agaaaatatc
     9121 ggattggtag aagaaggtca tgggtttaat attggagata agatatatat taaaaatgga
     9181 aattctaaga aagagataga ggtagcaggt atactttctt caatttcatt tgatgttaaa
     9241 gacaaatata ttggaaatat tataacatct gaaaatactt ttactagtat aacagatata
     9301 aaagactata taataattga tactcaagtt gatgaagatg ctcctgaaga tttaatccaa
     9361 aatataagaa atgttctaga tgataattta gaggttcgag ataagcgaca aggtaactta
     9421 gaggctaaaa attcatttta cactatggca gtttttgtat atgggtttgt atttataatt
     9481 gcgattataa gtatgtttaa tatcataaac agtatgaata taagcgttac aagtagaata
     9541 aattactatg gtattatgcg tgcaataggc atgtccaata agcaattaag aaaaatggta
     9601 atagtagaat caagtacata tgctgtaagt ggctgtttat tggggagtgt acttggatta
     9661 atattacata gatatatatt tgtatcctta gtaacattaa aattccatat agactggcaa
     9721 ataccttttg atttactgtt tatcattgta gtaactatga taataataac tttgcttgca
     9781 gtaagaaaac caattaaaaa gatttgtgaa ttagacataa tagaggtagt aaatgcacaa
     9841 taaatataag tagtaattta aatagaggta gtaatttaaa taaaaaatcg aagtaaatat
     9901 gaaatagaaa atatctttca taatttactt cgatttttgt gttatataat ttactttaga
     9961 taagttccat cttcatcttg ataaattaag tctttcttca taagtactcc aagagcacgt
    10021 ttaaaatttt cttacttgaa ttaaataaga tagaaatatc tttaggatca gatttatcat
    10081 tgaatctcat atatccatct gaaccttcta aatagcttaa aatcttattt tctaaggtat
    10141 caagttcatc ttttctgttt cctctaggag aaagtcctaa tttaccatca tcgtatattt
    10201 ttataacatg taaattttca agaacatccc cagcttttaa ttctgtaaag tattcattat
    10261 gaaggattac accagcatat ttattgtcaa cacatatcat agcagagttg ttggtttgga
    10321 atccatatac aactccattt acaacatctc ctacattgta agtatggtct gtttctaaat
    10381 atgaatctat gtctgtagta gcagctattc ttccgctttt atccaagtat atatagaata
    10441 aatatttttc atctctaaac aatggataag tcttagcttt aaaaggaact agtatgtctt
    10501 ttgtaagtcc catatctata aaagtaccaa tatccgtgtt atcaactact tttagatacg
    10561 taacatctcc atttctaatt atttctatag tattagaaaa ataattttta aatagtttgt
    10621 aaaatattat tgaatttttt ctctcgaaat atataattta gaatcttctg atttattttt
    10681 atgctcgatt tccttatttt ttttcataaa tatatataga ggagctatta acaacattgc
    10741 ccctaatcct attaaaactc tattttgagt tataggtttt gaaatatctt ctgcccaagc
    10801 ttgttgtaac aaccataagc tacctaaaat agctaccatt ggtataacag gaccaaatgg
    10861 aatcttaaaa gaggccttaa catctgttct ttttcttaaa actattactg ataaacaagt
    10921 tggtatgtac tgagcaaatc ttgcaattac actcattact gcaagttgtt caaaacttcc
    10981 agttagcaca agtggtatac aacaaattaa agatattatt attgctacat atggagcacc
    11041 atatttattt gtttttccta taaatgcagg aagagaacct tcttctgcta gagaagaacc
    11101 acatttagga gttactattg atgaacctat atttatacca aatatagata ttaatgttgc
    11161 tacagatatg aaaaatttac cataattgcc taaaaataca cttgaagcat ctgcaattgg
    11221 aatactgttt tcaaatagtc tatctccaag tatacccata cagacaattt gaattaatat
    11281 ataaattatt gcacatataa atattgtggt tattaatgcg actggaagat tttttttagg
    11341 attttccatt tctccacttg ccacaataaa tgattcaaag ccggtaaatg cgtaaaatac
    11401 taatataatg gctggaccca tactagagtt aactacattt cctgaaggaa caatatttac
    11461 aaattttata aagaatattc caactattat aaaaactatt aatggtacta atttagctat
    11521 agttattaca ttattcatta tctttgtgct tttaattcca aataagctat ttattgaaag
    11581 tagtgttacg agtattgcag ctatataacc tttgtattca gtggcagatt ctggccaaaa
    11641 tgaacctaaa gctgttgcaa atcctactgc tagagtagac catgatataa ttctaataac
    11701 ccaagacatt gtaccaactt caaatcctat aaaatctcca aaagcttttt tggagtatag
    11761 atatgctcct ccattcttat caaacatgct tccaacttct gcaaaacata aaagtattga
    11821 tagaactaag agtgtagcaa aaatgtatat aaacatactg ttttgacctg ctagattata
    11881 aactttacct ggtagtaaga atatacctga acctattatg ctattaatcc ctaataatat
    11941 catactaaat agacctaatt tcttagcttt ttccatatta atttatctcc tattctttta
    12001 ttaattactg tactaattaa aatacaaagt attcattaca tgcatcataa cacctattgg
    12061 taaacaatct tcatctatat taaagctaga ggtatgaaga ggtgaaatta agcccttttc
    12121 ctcattttta catcctaaat ggaagaatgc tcctttacaa tgttctgtgt aaaaagaaaa
    12181 gtcttcacca cctaaagacg ggtttggtct caatataaat ttttcctctc caagtaattc
    12241 tttagtattt gatataactg tatctactaa ttctttttca tttataacag caggatagtt
    12301 ttcatcactt acatgtaaag tcccaacaca accaaatgca gatgcagtgt ctccaacaat
    12361 tttagctatt ttatcaatca taaagtttct tgttttgcta tttaaggttc ttaaagtgcc
    12421 ttctagtttt acatcttcac atattacatt gtgtgcatca ccaccatata ttttacctat
    12481 agtcaaaaca gcagcattat ttggttctaa gtttctactt attatagttt gtaatgatgt
    12541 aacaatttgt gaagctgtaa ctattgcatc aataccattt tcaggatatg caccatgagc
    12601 tcttttacct ttgatactta tttttatagt gtctacacta gcatttaatg tatcatattt
    12661 tatttcgata aatcctgttt cgatatgtgg cataacatga agtccgaata tataatcgac
    12721 cttaggattt tctaaacatc catcttcaac taagaatttt gctcctccaa aaccttcttc
    12781 tgctggttga aataagaatt ttacatttac attaagttta tcttttattg aatgtaaaac
    12841 tttacatgct ccaagtagca tggttgtatg tgcatcatgg ccacaagcat gcatttttcc
    12901 agaatgtatt gatttataag gtatgttgtt ttcttcttca ataggaagag catccatatc
    12961 tgctcttata caaatcgttt tatctgcact ttcttttaaa atatatgcca taattccatt
    13021 gtgttttgta aattccatat aatcaattcc gatttctttt aaataagaaa ttaccttctg
    13081 tttagtaaga ttttcttcaa gagctaattc tggaatttta tgtaattctc ttcttatgtt
    13141 tataagccat ggttttatag aattacaact ttctattaca aaatcttttc ctaattcttt
    13201 ggtttgatat tttaatttca aagcctgttg catttttatt cctccaattg ctttttattg
    13261 ttttattaat ataataagca attattatgc cataattttc gatggagaac tttgtttttt
    13321 ataagaatat tgaataaata taatcattta tctatatgta tagtataaaa aatgattgca
    13381 tagtttcaat tttcagtaaa aaatattatt ttgaagaaaa tatattttat atgctagttt
    13441 atat
//
LOCUS       DAYAOY010000112        13126 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYAOY010000112 DAYAOY010000000
VERSION     DAYAOY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.112"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            214..1374
                     /locus_tag="ACGI6B_003351"
     CDS             214..1374
                     /locus_tag="ACGI6B_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9752765.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1716..1877
                     /locus_tag="ACGI6B_003352"
     CDS             1716..1877
                     /locus_tag="ACGI6B_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752766.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2070..2699
                     /locus_tag="ACGI6B_003353"
     CDS             2070..2699
                     /locus_tag="ACGI6B_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFG9752767.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2938..4197
                     /locus_tag="ACGI6B_003354"
     CDS             2938..4197
                     /locus_tag="ACGI6B_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFG9752768.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4468..5451
                     /locus_tag="ACGI6B_003355"
     CDS             4468..5451
                     /locus_tag="ACGI6B_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFG9752769.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5697..6764
                     /locus_tag="ACGI6B_003356"
     CDS             5697..6764
                     /locus_tag="ACGI6B_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFG9752770.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7298..9208
                     /locus_tag="ACGI6B_003357"
     CDS             7298..9208
                     /locus_tag="ACGI6B_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFG9752771.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9271..9894)
                     /gene="ytaF"
                     /locus_tag="ACGI6B_003358"
     CDS             complement(9271..9894)
                     /gene="ytaF"
                     /locus_tag="ACGI6B_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HFG9752772.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10082..10726
                     /locus_tag="ACGI6B_003359"
     CDS             10082..10726
                     /locus_tag="ACGI6B_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752773.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10940..11998
                     /locus_tag="ACGI6B_003360"
     CDS             10940..11998
                     /locus_tag="ACGI6B_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HFG9752774.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12102..>13126
                     /locus_tag="ACGI6B_003361"
     CDS             12102..>13126
                     /locus_tag="ACGI6B_003361"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9752775.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVK"
BASE COUNT         5419 a         1270 c         2157 g         4280 t
ORIGIN      
        1 aattgttgaa aaagatttta aattcagatt attcatttct aaagtaatta atgtgcaaat
       61 ctttattaat atatgtcaat agttaggtat atatgtattt aattagcatg atatagataa
      121 aagtatttat tttaattgtc tattatctga aaaagtcttt acttagaaat cataaaaatc
      181 aaattaatta ggaaaagaaa gggtgtgtat aatgtgattg agactggatt tgtatattgt
      241 agtttcttgg ttgtattcgt aggtgtaatt ttttttatgg ttgataaatt gaaatggaag
      301 gtatttgatg ttattaatcc aatgctttta atatacttag gttcagctat acttggatca
      361 tttggtttat ttgcacagaa tgaagatgtt ggaacgtatc aaaacataat aacagtgaac
      421 ttccttccat taatgttagt gtttttgtta attcaatgtg atttaagaaa aatattaaag
      481 atggggccta aaatgttatt atcatttttc tgtgctacat ttacatttat gcttggattt
      541 gttatagcat tcataatttt taaaaattca atgggtgcag attcatggaa aattcttggg
      601 gcagcatctg gttcatggat aggtggctca tcaaatatga ttgcagcagc taatgctcta
      661 ggagttgcag aagaaggact tagttatgca atacttatgg gttctatagg atatacagta
      721 tggatgtctg tttgtctaat gtctgtgaaa tttggaccta aatttaacaa atggacaaag
      781 tgcgatacaa gttttataga tgaggttaca tcaaaacttg aaaaagaaaa taaagataat
      841 acagcaccta cgtttgtaga acttatgatt ttactttctg ttgggtttgg agttgcgtct
      901 tttgcacaaa ttgtatccaa gatacttcca acttcaggta tacttaatga aactatgtgg
      961 gtagtacttg ttgcatctac agtatcaata ttattgggta tgacaaaaat atcaaagctt
     1021 aaagggagta cactggtagg aaatgtgttt atgtatttac tccttgcagc tacaggtagt
     1081 aaagcaaatt tctttggatt gtcagaagca cctgtttaca taatgtttgg attggtagta
     1141 gtccttgtac atgctatttc atttataggg cttgctaaac tatttaaact tgatttattt
     1201 acttgtgaag taggaagtat agctaatata ggaggagtaa ctacagctcc aatgattgct
     1261 ggggttcaca atcctgtatt aataccagta ggtgtactta tggggcttct aggaaatatt
     1321 atagggactt attgtgctct tatggtaact caaatattac atatgttagc ttaataaaaa
     1381 tatggtataa aattaataat ttaaataaga aaagtcattt caatttaagc agaagctttt
     1441 actgaaatgg cttttttgtt tgacaagata atctatttca agaagatgtt ttgacatatt
     1501 tagtgtataa tattgtaaaa tttactaaaa acgagatata tttaattttt atattgaatc
     1561 gaacgtctgt atgatgtata ataaatatac agaacgaatg tttgcagaac gggtgttttt
     1621 tgaatttatt ttaataattt atgaatatat atcagaatgc ataaatatat ttaaatataa
     1681 agattaaaaa gaatttaaag gagtgttgat aacttatgat aattccatca agaagaatac
     1741 aagatataac attaacaaat ttaaaaaatg gtgaagtaac tttaattgaa ttggatgaaa
     1801 tatacaagaa gatgggattt ttatttgttg ttagtgaagg caaattgaaa aagataaaaa
     1861 aggagaatca tcattagaaa tgaatgaaat aataaatatt ataatagttt ataaaaccta
     1921 acttatgtag aatataaata cttaagttag gtttttttgt gataaagttt ataagtactc
     1981 aattttataa gataaataat ggtcttaagt gaaaagtaaa taagtttaaa ttttagtaag
     2041 tattatattg cataagaggt gatattatta tgaaaatagt aacctacaat attcataaag
     2101 gtatggattc aaataataga ttaactctaa ctaaaatggg gttatattta aaacaattgg
     2161 attgtgatgt aatttgtttg caagaagtat tatatcctca atttttagct ttaaaagctg
     2221 ttttaaatat ggatggtgtc tttgcgacaa atgtaaaaaa agtaaatatg atatatggta
     2281 tttgtacatt tacaacatct aaaatgttaa acagtaatca ttttttctta actagcaaaa
     2341 aagaacaacg gggagctctt tgtataacta tagatgcata tggaagaatt ataaatgtaa
     2401 taaatactca cttgggactt gataggcagg agagagcaaa acaattagac gaaataatag
     2461 actataggaa cagattagtg gggatagtta ttttatgtgg tgattttaat gagaaaaatg
     2521 tctttttaag tatgtttaat gatatggcta tttcactaaa caagtcttac ttaccaacat
     2581 ttgaaaaatc aaactctaga atagattaca tttttgttaa taaaaatact gaactaaaag
     2641 gatacacagt agaaaaaatt tacctgtcag accattatcc tgtaataggt tacatttaaa
     2701 tttcaatata aatgaaatta gttgttataa aataaacaat tttttgttaa gaaatagtaa
     2761 aagaaatata ataaaaaata tcaaaaataa aactaattat aagacataaa tttacaataa
     2821 agttacattt gattaaattc cttgtttaac aaaagctatt caaatataat ctaaggtagt
     2881 tttgaaatgg gaactctatt atgcaaattt aagctagtgt taaaatgagg tgaagttatg
     2941 ggcagaagaa caacaagagt ttcaagatta ggtaaagaaa aaaaacgaag tgttttaatt
     3001 gtattggcag ctttagttct aataatgggt ggatttactt atttttttaa cagtaaattt
     3061 ctgtacaatg ggaaaatcgc taaaaatgta tatatagaag gagtaaatgt atctgatatg
     3121 actaaagcag aggcattaaa agctataaca gataagtata ctccagaaga tttaaatctt
     3181 gcttatgatg ggaaaaaata tacaatatca ccaaaagata ttgatttaaa atatgataca
     3241 gaagatgttg taaaagatgc ttatgaaagt acaaaaaaag ggtcctattt tcaaaactta
     3301 aaaaaatata ttgatataag agtgaataag gcaaatatga agataaaagc tgaatatgat
     3361 gaggcaaaac ttagttccaa agtgtcttct atagcagata gtataaatgt taagatgaaa
     3421 aatgcttcta ttagtgttgg aagtggtgga ctaagttata cagactctgt tgtaggtaga
     3481 gaatttgatt tggcagcaaa taaagagtct atatataata tgataaaaaa taaagagcat
     3541 aaaactttag aacttaaagt aaatcttcaa aaaccagata taacaactga acaagtaaaa
     3601 actgtaaatt ctgttatagg tcaatattca actacttatt cacaagctgt agagggtagg
     3661 tcatataatg taggattatc agcaagaaaa actagtgatg tattattaat gccaggtgaa
     3721 gaattttcat acaataaact tacaggacct agtaataaag caaatggtta caaagatgca
     3781 cctgttatag tttatggtaa gctagaacaa tcagcaggag gaggtgtttg tcaaacatct
     3841 tcaacagttt ataatgcggc tcttctttcg ggtatggaga taactcaagt tacaaatcat
     3901 tcatctgcat ctacttatgt gcctaaaggg agagatgcta ctgtgagcga tggaggatta
     3961 aacttaaagt ttaaaaatcc atataagcat cctgtatata ttaaaaatta tgcaggtgga
     4021 ggaagtgtat catcagtaat ctatggtaat tctggtgaca aaccaaatat atctatagag
     4081 gtaaaacaaa ctggtcagaa taaatattct acatatcgaa tatttaaaga ttcaagtgga
     4141 aaggtcataa aaaaagaaca tatatcaaat agttcttata aagaattaaa aaaatagata
     4201 attttttaga gaaatatttg taatataaaa gggagtgtct caaaatgaac tttttagttt
     4261 atgagacact ctttttctgt gaaatcaaat atattttcat catttcaaca atacaaaaga
     4321 agcgtatctc tattttgaga cagccttttt tgtaaaaatt aacactatta gttcttgttt
     4381 taatattaca atcattaata tatagaaaat attcctatat attggtataa ttgtaaatag
     4441 atttagttat ataaaatgta tgaggaggtg acatggttga attttatagg caatagatat
     4501 gaggtagtaa attctgacga cgttttagaa attaacaaaa tctataaagc tagagatgtt
     4561 ttttacagga aaaatgtgtt aataaaagtt ataaagcaca ataattatat ctgtgaagat
     4621 tttgtttcaa atttaattga tgaaagtact gctttagatg agataaactc tccttatata
     4681 ctaaaaataa tagatgttgg gattcactgt acagaagaaa caacattata ttatgttgta
     4741 agtgaagact ttaatggtat cggactagat gaattaattc taggtaatta tcttcattta
     4801 gaagcaattg taaacataat gatacaagtt ttaaaagctt tagaaatgat acataggaat
     4861 cattcatatc atggtagttt aaaatcgagt agtataatag tagatacaga atacaatata
     4921 aaaatttgtg attttggaat tacaaaagca aataatgggg ttaatacaag aagctatgga
     4981 aacaggagat atttatgccc acatcaacta tgtataaatt atactgataa agaaagtgat
     5041 ttttttgcaa caggtcttat tctatttgaa tctatattta aaaaattgcc ttttggagaa
     5101 tcaaattcag aagaaaaaat gctacaatct attgataagg gattagattg gaatagtata
     5161 aaagcaatta atggaaatac tgaacttata aatgttataa aaaggcttct tggaagaaat
     5221 aataagtata ctagagccaa tgatattatt attgatttaa gtaaagtaat gtatgaaaaa
     5281 gcatatatag aagttgaaga tgagaaagaa gaagagcaga aacaagacaa agttatacaa
     5341 gttaaaaaag aatataaaag taaaatgaga aaaaaattac ataaaaaact tgttgttgcc
     5401 ggtctagcta tagtcatgat ttcaatgatt attattagtt ccataatata aaaattgtat
     5461 attaagtatt ttaaaatcaa tgtctataaa ggatatacat agaaatttta gtgtagtctt
     5521 aaattatgaa aggaagtggt tattatgtta acaaaaaata atgaggtatt aaacgtctag
     5581 gagtatttta acaaatagcc acctgctttt acagtttaga ataaaaaagc tgtggctaaa
     5641 ggtcacagct ttttgctact ttttaaaggc aggagaatat taaaaaggag caaattatga
     5701 gcaatataaa aataatagca agtaataaat gttggattga agatatagca aggaaacaac
     5761 ttgaagatat ttcaaagtta aatggaattt taagagtagt aggattacct gacttacatg
     5821 caggtaaaat acctgtaggt ttagcagttg agactaaaaa tattatttat ccacatataa
     5881 ttggaaatga tataggatgc ggaatgactt tatttaaaac tggggttcta aaaaagaaat
     5941 ttaaaaaaga caaatggatt aagtcactta gtaagattaa agatttgtct gatatagaaa
     6001 taaaaaacac atataaagaa gaatgtccaa ttttaaattt aggaactata gggagtggaa
     6061 atcattttgc agaaattcaa tgtataagtg aaatttataa taaagaacaa tttgaacaat
     6121 taaaattttc atctgatgat attatgatgt tagttcactg tgggtcaaga aattatggag
     6181 aagatatttt aaaaaaattt tatgataaag atggacttga agttgaaagt gagaaagcta
     6241 tagattatat taaaaaacat gataatgctt taatgtgggc agagcgtaat cgtgaagcta
     6301 tcagtaaaaa aattatgcag tcaataggta catctggaga tgttgaaaca atattctcta
     6361 taaatcataa ttttatagag aaaagggaag ataaatttat acatagaaaa ggtgctgttt
     6421 caagtgaaag aggagctgtg attataccag gttcaagagg cagtctatca tatatagtta
     6481 tgcctactga aaatacagag atatctttat attctctatc acatggagca ggtagaaaat
     6541 ggtctcgttc agtttgtaaa tctcgcctta aaagtaagta tagtaaagat actataaaac
     6601 aaactaaatt taaaagtcag attatatgta atgatgtaaa tttattattt caagaagctc
     6661 cagaggcata taagaatata gagcagatta ttgaaagttt gatagaatat gaacttattc
     6721 aagtagtggc aactatgaag ccattgatta cttataaagg ataattttta aagttaaaaa
     6781 agattaaaat tatattataa atagctaatt ttatggaaca tttaaaagtg ttttattatt
     6841 agctattttt ttattttgta gagacgataa tttatagagc taaattcttt gatatataat
     6901 gattacaaag ttagactgta ttggaaactg cacatacatg aattgatgac aatataagtg
     6961 attcccaaat ataaatgaaa gtataagctt atttttacta taaaaatttt cactgtacaa
     7021 aaacattaat gacattattt ttattgacat aaatatataa tttatggaat aatattagaa
     7081 gcactgatat aagtgtttaa ataagaatag aaaaggagaa gtgttttatg aaaaaaaata
     7141 ctaacattaa tgagtccgga gttaaacttc ttaataaata aggagtttga tatataataa
     7201 ctccttattt attaagttgt tcgcaaaata actattatca aagtaaatga tagggtgatt
     7261 taaaagacga tgacttataa ataaaggaga aaaaataatg aatagtaata catttgaaag
     7321 attacaacta aatgaagtta aagacttaat aaaaatacat tgtgtaagtt cgctaggaaa
     7381 aaatttaata gataaattaa ctcctagtgg gaatattgga gtagtaagaa ggaaattagc
     7441 agaaaataaa gaagctagaa agataattga aaatagtaat cacataccac ttgaaggatt
     7501 gtttaatgta ggttctacaa tagataaaat tgaaaaagga atgataatag aacctgttga
     7561 acttgtaaat attgaagatt ttttaagagg ttgtaggaag atgaaagctt ttatgttaga
     7621 aaaagaattt tattcaccta cattaagttc ttatgcctta aatataacag aatgtaaaag
     7681 tatagaagat gagataaatt actgtataaa atctaataaa gttgattcta atgctagtaa
     7741 agaattaaaa aagataagac gaaatataga aattacagaa ggaaagataa aagatagatt
     7801 aaataaattt ataacatcaa ctgtaaataa aaagtatata caagagttta taataagtaa
     7861 aagaaatgat agatatgtaa taccaattaa atcttcttat aaaaatgaag ttaatggtac
     7921 tatattagat acatcatcaa aaggaaatac tgtatttatt gaacctatta gtgtttcaaa
     7981 tttaagtaca gagttaacta tgctaaaagc tgatgaaact attgaagaat acaaaatatt
     8041 atcttattta acagaactta tatttgagaa gataagtcag ataaaattaa atatagagat
     8101 actttcagaa tacgatatgg tatttgcaaa agctaagtat agtcaaaaaa taaaaggaat
     8161 tactccaaaa ataaataata atggctatat aaaaataatt aaaggtaaac atccacttct
     8221 aacaggagat gcagtacctt tagattttga aataggcaaa aattatagaa gtcttataat
     8281 aacaggtcct aatgcaggag gaaaaactgt aacccttaag actgttggtt tactaacttt
     8341 aatggttcaa tgtggatttg atatttctgc aaaagaaggt agtgaattta gtgtatttga
     8401 aaaagttttt gtagatatag gggataatca aagtattgaa aatgccttaa gtactttttc
     8461 atcacatata aaaaatattg cagagattat gagtttatca aataattcta ctcttgttct
     8521 gtttgatgaa ataggaagtg gaactgaacc taatgaagga gctggtttag caatatcatt
     8581 attagaagag ttttacaaaa tgggatgtat aactatagca tcaactcatt atggagagat
     8641 aaaaaagttt gctacattgc atccagaatt tgaaaatgct ggtatgatgt ttgataaaga
     8701 aacgttagaa ccattgtata agttaactat tggaaaatct gaagatagta atgctctttt
     8761 tatttctaaa aaaatgggaa taaaaaatag agttttagat agagctaaag aatatgtaat
     8821 tgatagaaac tacaatttag atttagttaa aagaagtaag ctacaatcta atattgaaga
     8881 taaaaatagt tctgaagtca ttttaaacta tacagattat aatgtagggg ataaggtaaa
     8941 attactagat gaagatgatt ttggaattgt atataaacct atggacaagt ttaataatgt
     9001 agaagtattg tttaatgata attttatttc agtaaatata aaaagattac aacttagcat
     9061 aaaagcatca gaattgtatc cagaagggta tgatttagat gtattattta ctagttttaa
     9121 agaaagaaaa ttagaaaaag atattcaaag aggttcaaaa aaagctctta aaaagattca
     9181 gaaagaaatt agagagaata gaaaataaat ataaaaagca caaaagaaag caatctaaag
     9241 agaaaagttt atctttagat tgctttctta ttaacatatc ttcataaatc caatgactaa
     9301 caagattaaa ccacttaacc aagataagtt taaatttacg tttttaacaa acatatttcc
     9361 tagtaaacaa ccaagtaata ctgcaattat atttgatatt aaagaaaata gaataacttg
     9421 tatataattc acacctacaa ggccactacc aaaaccgata gctaatccat ctaaacttaa
     9481 agcaagtgca agtgtaaatg cttcagcaga tttaagcact ttagatttac ttgtatctaa
     9541 gacatcaggt tcgatactta cattaaaatt taatttaagg tctaacatat taaaattata
     9601 attctttgag tgtatagatt ttttcttaaa ataagattta aaaaaacttt caattagtct
     9661 agcacttcct aataaaaata aaataaagaa acatattaac attgttatat tttgaggtaa
     9721 aaagtctcta actattgtac ctgcaaaaag cgatattgct aaaatagctg aacaaactac
     9781 gctgataatt atatttgaat ataagggaac tttaacttta tttacgccat atccaaagct
     9841 tgtcacaaag gcatctatac aaagagaagt aactaataaa attgattcaa tcacagtaga
     9901 ttaacatcct ttctcttaaa ttttgtactt atattagtta tagtattgta aaaatggata
     9961 aattgttaca gataatccaa taaattgttg ataaaattcc aacatcatac aacacctaaa
    10021 taacaaaaga atcatatcat ataataatat aaaaccaatc ataataaagc gggtgaaaat
    10081 aatggaacaa aataataagg ttgggaattt cggtgatttg ttgaagattt tattagatga
    10141 aaaagaatta tcaatgggag aactaagtaa aaaaagtgga attgataaat ctactatatc
    10201 tcgtatagct aataataaac aaaagccaaa tataaatcat ttagaaaaga tggcaataca
    10261 tttaaatatt aacctagaag aactgttgaa agcttcagga tatgaattta gaaacagcaa
    10321 taatcaaata tttaatgcag atagtgattt tagtaacttt gatgatattc ttggatttgc
    10381 aaatttaatc aatgataaga actttaatgg aaatattgaa aaagagttaa gtaagtgtaa
    10441 gttatatgtc caaacagatg aaggtaaaaa attacttttt gataatttta ataaaaaaat
    10501 agattctatt gaaaaacaag gtcaatttac agatagatta agacaaatgt atgctgattt
    10561 ttgtacagat ggacttacaa taaagaaata tttattgatt ggaagtatgc tactttattt
    10621 tgttatatca acagatgtga ttccagactt cgtattccca ataggattta tggatgattt
    10681 agtagcatta aatatagtta caaaattact taaaaatgat aagtaagttt tagaatcata
    10741 tttaatatat aaataaatca aagtatataa aatttattat tttttatatg aaacaaatta
    10801 aaagcacaat gaagtagatt aaaaatttaa aactaagaat ctaatatata ttttgtaact
    10861 atttaagtat ataaaataaa ataatatcca aactaataca aaatagtaat aattgttttg
    10921 tactggagga tatatattta tgaattgtaa atggatatct aaaatagatg agagaaaaaa
    10981 atgtcataga gaagcagatt cttctggata ttgtatcttt cataaagaaa ataagtctga
    11041 tgaagaaata cagttgatga tggatactat tcataaagaa gagataagtg aatttaatgg
    11101 atttgttttt gaaaatgaat ttaatgcgga agaaatatta acttacaatt ataaaatact
    11161 ggatttttct gaatccatat ttaaacaaaa agcaaatttt aaaaaatata tttttaaaaa
    11221 gaatattatt tttaactata cagaatttag agataaagtt ttatttaatg gttgtgtatt
    11281 cttagaaaac tgtgatttta atagaagcat atttagtaaa aactatataa atgaccgtat
    11341 atttgaaaaa gtgaaattta aaggacctga tttggtagtt aataaagtag aaaattttcc
    11401 aagaatggat ggaataattt ttagcatgtg tacaaaattt gtgttaaaga atgttgaata
    11461 tggaaaaagt gagtatgagc atggcaagat aaattataga atagctagaa atcaagcaac
    11521 aaaaattgga gaatatgaga tgataggatt ttactattat aaggaaagaa tttatagtag
    11581 taaaataatg aaacgttcta attatcctac tttcagtgat tatttagttg aaaaattttt
    11641 tgaccaaata gcacgttaca caacaggtta tggagaaaag ccatggaata tattattagt
    11701 tattatagca ataataagtg tttttgcttt attatactta tttgtaggca tagaaagttc
    11761 aaatagcaca cttgtagctt tagatataaa taatataggt gattactcac taagtgagat
    11821 tttcaaaatg tatatggatt tatggtattt tagcatggct acatttagta cagtaggata
    11881 tggagatatg gttgctacaa gtttgattgg gaaagcattg gcaggtatag aagtattttt
    11941 tggagttaca ataggagcaa tttgggcatc tgtgattatt aaaagaatga taaggtaata
    12001 tttgtgtaaa tatggatagg tattaattac tagatataaa ttggactaaa tagggaaaat
    12061 ttaaatataa atatacttaa taatccaaga aggtgaaaaa tttgaaaaat gcactaaaaa
    12121 aaattatatc attagccatg attctagttt ttataatacc aaattattca tatgcagatg
    12181 ataaatctgt agacaagtat tcaaaaacat ctatacttat agaccaagaa acaggtagag
    12241 ttttatatag taaagaacct gatatgaagg taccattagc tagtttaagt aagatgatga
    12301 cttttttact tgcaatagaa gctattgaaa ataaagaagt aaaagaaact gataaagtaa
    12361 aaatagacaa atctattgca tcagtaaaag ggtcaagtta taaacttaaa gatggtgaag
    12421 aagtgccact tatagagctt atgagaggtc ttatgattgt atcaggaaat gatgcggcaa
    12481 ttgctatagc aaaacatatt tgtaaaacga aagaagcatt tgtagataga atgaataaaa
    12541 aagcaaaaga aataggaatg agtaatacac attttctaaa tccaaatggt cttccaatat
    12601 acgatttaag caaccccaaa aaaccagcaa aagaaaatat ctctactgca aaagatatag
    12661 ctattcttgg aaaatacatg tttgaccatt atgaaaaaca agtaacagct ataactgata
    12721 tggagacata tacaaattca gatagaagtt ttgaaaaaag caatacaaat gcattattaa
    12781 gaattatacc agaagtagat ggtataaaaa ctggatatac tggcaatgct ggatattgct
    12841 tatcattttc tatgattgta aataaaaatg ataaaaatga gaaaaaacgt agagtaatag
    12901 gggttgtatt aggtactaac cataaaaata aaagaacctc tgcttcatta gctctactga
    12961 aatatggaaa agaaaatttc aatatgaaaa aagtaattga taaagatagc tttattggta
    13021 agaaatatat aaaaggaatg aaagagttag aggttacatt aaaagcaaaa gatgaacttt
    13081 atacaatatt aaaagatgat gaaagtataa aatcagaagt taagat
//
LOCUS       DAYAOY010000113        12984 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYAOY010000113 DAYAOY010000000
VERSION     DAYAOY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.113"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            203..1375
                     /locus_tag="ACGI6B_003362"
     CDS             203..1375
                     /locus_tag="ACGI6B_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9752776.1"
                     /translation="MIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1711..2280)
                     /locus_tag="ACGI6B_003363"
     CDS             complement(1711..2280)
                     /locus_tag="ACGI6B_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFG9752777.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2683..3255
                     /locus_tag="ACGI6B_003364"
     CDS             2683..3255
                     /locus_tag="ACGI6B_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HFG9752778.1"
                     /translation="MEYIINPCSGIKIKITRGQKIEIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLSKANDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(3934..4836)
                     /locus_tag="ACGI6B_003365"
     CDS             complement(3934..4836)
                     /locus_tag="ACGI6B_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9752779.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(4963..5082)
                     /locus_tag="ACGI6B_003366"
                     /pseudo
     CDS             complement(4963..5082)
                     /locus_tag="ACGI6B_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(5625..6218)
                     /locus_tag="ACGI6B_003367"
     CDS             complement(5625..6218)
                     /locus_tag="ACGI6B_003367"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9752780.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(6450..6986)
                     /locus_tag="ACGI6B_003368"
     CDS             complement(6450..6986)
                     /locus_tag="ACGI6B_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFG9752781.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7059..7472)
                     /locus_tag="ACGI6B_003369"
     CDS             complement(7059..7472)
                     /locus_tag="ACGI6B_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9752782.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            7938..8492
                     /locus_tag="ACGI6B_003370"
     CDS             7938..8492
                     /locus_tag="ACGI6B_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFG9752783.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(8738..8893)
                     /locus_tag="ACGI6B_003371"
     CDS             complement(8738..8893)
                     /locus_tag="ACGI6B_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752784.1"
                     /translation="MVKFNQSLKVSKEKIITLKQEDINGKMKTRFYPFISDKGMCFNQ
                     RWTFILG"
     gene            complement(9100..9303)
                     /locus_tag="ACGI6B_003372"
     CDS             complement(9100..9303)
                     /locus_tag="ACGI6B_003372"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9752785.1"
                     /translation="MCLDAVIYMLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDI
                     VFSCIVFHHCTNVQNIFCIFWKW"
     gene            complement(9505..10038)
                     /locus_tag="ACGI6B_003373"
     CDS             complement(9505..10038)
                     /locus_tag="ACGI6B_003373"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFG9752786.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10355..10486
                     /locus_tag="ACGI6B_003374"
     CDS             10355..10486
                     /locus_tag="ACGI6B_003374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752787.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYN"
     gene            10805..10993
                     /locus_tag="ACGI6B_003375"
     CDS             10805..10993
                     /locus_tag="ACGI6B_003375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752788.1"
                     /translation="MPYKYFTEFCLLKATQFLKGTNELISDIAIKVVFHQIIYFSKCF
                     KEKTGYSPKVYRNIKKEI"
     gene            complement(11786..12358)
                     /locus_tag="ACGI6B_003376"
     CDS             complement(11786..12358)
                     /locus_tag="ACGI6B_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9752789.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12420..12683)
                     /locus_tag="ACGI6B_003377"
     CDS             complement(12420..12683)
                     /locus_tag="ACGI6B_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFG9752790.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12683..12904)
                     /locus_tag="ACGI6B_003378"
     CDS             complement(12683..12904)
                     /locus_tag="ACGI6B_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFG9752791.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         4545 a         2218 c         1544 g         4677 t
ORIGIN      
        1 agtttccaga atcttaatta atagaaaggt atattaaatg ggcacttatt ctttaatctc
       61 tacaagcata aattttattc taatattaat caatattttc atagcctttt ttctttataa
      121 gttgacttcc tattttatag actttaggaa tcattggatt ttcaaaatca tattgatttt
      181 gggctactct attattagtc aaatgataat atggctttcc gaccccataa atattttgtt
      241 tacactattc ggatatttta tactacttat aatatgtagc aaaagcaaat taattcctat
      301 aatatcagtt gtcatgatac tttatcctat tataattggg cttaattttc tttttataaa
      361 caaccctata tatgaggaac ttatacactc aaaagagtat attttaatac tttcaatagt
      421 ccagataatt tctgctctaa ttaaattatt attttggttt ttaatacata ttttcctaaa
      481 gaatagattg gtcaatataa aacaatactt atcaaataaa atatggattt ttattgatat
      541 ggtatgtata tcttcatttt tatcaatatt aattgctatt attctgccac ctactaattt
      601 aatccctatg ggtagtaata agaatattat cttaggcaac ttaccttatg gcacttatct
      661 aatcatcttg tcagcaattc tggcaaatat cggtgtgata cttcttttac agccattgat
      721 tgaaaatgta aagatgaaga tagaaaaaca agcaaatcac ctaaaagaaa aatactataa
      781 cttactagaa aatcaacaaa ttcaaatacg caaaatgcga catgatatga ataatcactt
      841 tcaaatgatt gaatcgtatt tagaaattga agatattgct ggagctaaaa agtattttaa
      901 tcagttaaaa ataggtatag gtactcttag tggaaaacaa ttttgcaaaa accaagctct
      961 taactcaatt ttaaatcttc gtcatacaag tttagaagaa aatggtgttg atgtgcactt
     1021 taatatagat atagacaatc acttgggtat agaacctata gatttatgta ctattttttc
     1081 aaattcttta gataacgcta tagaagcatc tttgaaaatt cctgatttgt caaatagaaa
     1141 agtggtatta aaggcacgtt gtgagaagga gtattttagc tatttattga caaacaacaa
     1201 ggtaaatcct atcaatagaa aacaagaact atttatttca gataaaaaag atagtaattc
     1261 tcacggatat ggtattgaga atattagaga aattgttaat aagtacaatg gaaaattgga
     1321 tatttcttat actgagagtg aattttcttt atttctttat attagattat tttagtctaa
     1381 aattagatta tcttatataa tacaaattag attgtttgat actatgtaaa attataataa
     1441 cttataatta aatatttaca catatattca ttaacttaaa atttaatcaa atgtgatatt
     1501 ttatttacct tagtaaaaat gtgtgtgtct caaaataaac tttttagttg atgaggcact
     1561 cttttttaca tgaaatcaaa tatattttta tcctttcaac aatgaaaaat ggatttatct
     1621 ctccattttg aaacaagccc tttttctatt aattactttc ttaaaaaata taatacgata
     1681 tataagataa atatctaatt gtacctattt ctaattatat ctattagaat acaacacctt
     1741 tgccttttca gtaaactccc taacaaaatc tagtttacta tctgtataag catcacgatt
     1801 atgctcatac ttcttccaaa ggtccaactt aagtttttca tactcctttg caacatctgg
     1861 gtggtcaatt aagtaatctc taaaaaacaa ctcatcatta tcacctacat accttatatg
     1921 caaatgaaaa actcgttctt caaacccctc tactgtatac cctttattaa gtgaaagcct
     1981 actactgctc tcagacatac aaatatatcc attcgactca atcaaatctt taatatcttt
     2041 catattataa tcaggaatta tctcaatcag tatgtcaaca atgggcttgg cataaatcgt
     2101 actcacagaa gtacttccaa tatgacttat tctaacaatt ttttgcattg gcaatattcc
     2161 agttagtcta tattgttctt ctttaaacca atttttccaa cattctttgt gagtagtcaa
     2221 aaatatagga aacaagttcc ataactcctc taaagtcata tcttccaact tctttttcat
     2281 tagttacctc cttttaaatt tgtactttat attttttctt tttcaaattt acttagttaa
     2341 tatttaactt tattttttat caaatcattt ttctaattat gtaaattttt ctaaatttag
     2401 tttttaatgt attataacag atttttaccc ttcttacact tttgtatcta tttaaatatt
     2461 agaacatttt ttcttacttc taaatacagt aagattgctt aaataccaaa taggagcaaa
     2521 aaaactctgt aatctaaaaa aaactacaac atctttatga gaattaaaaa atgatagact
     2581 atgttatcaa cattaagaaa cagatagaaa agaaacagag tatgtgctaa aatgggaaca
     2641 tagaacagaa tgacaaattg gaatttatgg aggacattaa atatggagta tataatcaat
     2701 ccttgtagtg gtataaaaat taaaataact agagggcaga aaattgaaat tgtggatatt
     2761 gaaggaggac aggtcgttga tttttttgca gaaatgaaag atgattcaac tgaatttata
     2821 tctccaggtg ttacaattga ttgtaatgag tcattaagat tacaagttgg gaatttaata
     2881 tattctaata agtatagacc tatgtttaaa attttatatg atgatgtaag ggaacatgac
     2941 ttgctacatc catgttgtag acctgagatg tacgatttct tttatcacaa tggagaaaat
     3001 catccaaact gttttgataa tattaatcgc acacttggaa cttcccatca aattattcac
     3061 cccattaatt tgtttatgta cacaaaagtc aatacagatg ggagtattga agtatgtgaa
     3121 cctttatcaa aagccaatga caaaattatt cttgaagcat tggaaaacat gactttaggg
     3181 attgctgcat gcagtgtttc agaaagttct tgtaatagca gaaaatgtac ttctattaag
     3241 attttggttg attgacaaat ttcatttatc gaaaaaatat caaaattaaa aaatgacgtc
     3301 aaaggcgcaa aagggtaacc acttgcgctc tcaattcatt atggtttgtt acgtatttat
     3361 gggtaaaaat cccttgattc tatgatgttt ttcagacaca attacaaatg agtaaggatt
     3421 ttatactcat actctaaaaa atagcatttt attgcgtaac agaacgaaaa aacagtgtgt
     3481 acctttttct tgcaatacac actgtattca tagccactct agctgttaac tgttaattat
     3541 ctcataatac ccttactggt attcatctaa tcttaacccc ttttaattat tttcatttat
     3601 aaataaattc tattcttccc aaaatatcgt aattaaaata tattaattaa aatatcccaa
     3661 taagaatatc tgaaaaatct tctacctatt ttttcaaacc ttttattccc attcctcata
     3721 caatagtgca acttttagca cagtataaat ataccattag tccatataat ctcattaaca
     3781 aatatataca attattttct tcaaactctt ttatatctaa gatatcacat tttcattttc
     3841 ttatattaat tccttaactc tctttcaaat taccaatttt ttaatcaaat cttcattaaa
     3901 tattctatca ttattaaaca ttcatcatta tccctaaaaa atatgtgccg tatgttcttt
     3961 acacaaattt tccatcttat catacacagc accactatct ctctcatctg tcatagtcac
     4021 aattacatca cttggtctaa gaattgtctt acctctagga ataatttctt ctccacctct
     4081 ttgaattgct accaaaagac aattttcagg ccactgtaca tcctgaattg ccttgttcgc
     4141 aacaggagag ccaaacataa cagagaactg gtctagtatc ttttcgcccc tctctttatg
     4201 tacaggttct ccattctttt caagtaacct ctcaagaaga ctttcataaa taggtttaga
     4261 cttcataagt gtagccacaa tatatgccac aattgaaatt actgatagtg aaagcatttg
     4321 actaacagaa cctgtcattt caaaaattaa tatgatacca gtcaaagggg cacgcacaat
     4381 agctgtaaaa tatcctgcca tagccaaaag cacaaagtta tttacataat cgggattcat
     4441 tccaaaaact ttaacaccta ccataccaaa tattccacct ataaatgctc ctaaaacaag
     4501 caatggaaaa aatattcccc ctggtgcacc tgacccaaaa ctaacagccg aaaatataaa
     4561 acgtataaca aaaatcaaaa caaccattcc aaaaatatat ttatgacttg taagttcttc
     4621 aactaaattg tgtccactcc ccagaacatc aggcataaca agcccaagaa taccagccat
     4681 tataaatgga atcaacagtt ttccaaacgt accaatttta ggaatcttct tgtataaaga
     4741 ctgcacatat agagtaaacc agttgtaaaa tgcccccata actccaagaa taacacctaa
     4801 aatcactatt aatccataat aatcttgtgg caacacattt ccaatctgaa cctcccatga
     4861 ctaaagtcac agggttcctg cttcatagaa ttttgcatac taaaatatgt cttactaatt
     4921 ctccacaggc tatccccgta gttcctacgg ttcttacata agttatttat tttgaattgt
     4981 tattagtctt aatccttctt ttaatatatt tatacttgca tttatatctc tatcgtgagt
     5041 ttcattacaa ctaggacaaa tccattctct tataataatg ttaacaaatg taattattta
     5101 aaacaattat ttagaaggtt ttatatttaa ttttaaataa catatttaag aaccgtaagt
     5161 cttaactttt atcatttttg aggttgtcgt tcacataagt tcgctactac ctatgcagtt
     5221 ctcttatgaa cttcttacac tttcatgcaa gcacagacta tatcttatcc ttcggcatta
     5281 tccgttaagg tctacccact tccactacca atagcttgta gtgtacttcc ctcaagagga
     5341 atagtcgttg aagtttctcc tattcggagt ttacctgctg attgcccatt ttaaagtact
     5401 tagggtttaa ccttatacca tctaaccaat tttttctact ttcgtaactt tcacacttag
     5461 gtttatttca tccttatgtt ttagtttggt tagctttagg ggtttccagc aattcaagta
     5521 gtattggata ggttctatcc tatctctaca tataggtttc cctatatgct gactatttta
     5581 gctactaact catgactaaa gtcacaagtg ttcgtagcta ttttttaatc aaactggaat
     5641 acagactcca ttcctaggaa attggctgaa atatagtctg ctgtcaaaga tgctgtcatc
     5701 acagaaatta aaacagatac tgaaaagttt ttatggactt cttcaagtga aaacataaca
     5761 cccgctagtg gtgcatgaaa ggctgctgat aatccagcac ttgccccaca ggttaacagg
     5821 aatttttcct ctgtcttccc tcggtcaaga gctctagaaa tacctttacc tgccatagca
     5881 ccaagctgaa tggaaggacc ttcccttccc aaagcaagac cacccatgag tgataagaat
     5941 cctccaataa attttgttgg caacactcgc caccatactt gttttatttt ccctgtcatt
     6001 tccccctcaa gctgagggat accacttcca gaaatcattg gctcccagtc caccaacttt
     6061 gatacaatcc acgcaagtac tgcaagtact gcaaaccaag ctattatttt tattgtagaa
     6121 ccttctacaa attttaaaat ttggctaagc caagtaccag cctgccctaa aagtatcctg
     6181 tataaaagca caatcaaacc agccacagcc ccaaccataa gtccttcacc aatcaaaatt
     6241 acctgaaagt gctctgcccg tttgataata tgagatgtat cctttttcat actattcctc
     6301 cttcatccaa taattctaca caatctgaat ctccaatgac taaagtcatg agtgtgtaca
     6361 gatatttttt aatcaacaaa aatagatttt tacttactaa atataagaaa ataattgctc
     6421 aatttctcaa taaatgtgaa tttattcttt taggtattta ttctccacag tcgtgcttct
     6481 tccagcaaaa tatttattaa ttcttgcttt ctctgtgtat agattttacg attgtcctca
     6541 aactgtaatg ctaatttttg tttatgacta tcatagagtt ttgctttgtc tgtaaaagta
     6601 tttagataat ctcgaaaatt gatataatta ttccatgcaa cactattcca ttcaacaaca
     6661 tgaatatggt gagtacgagt atttttctca aaatctccca tagcaaaaag taattgtttt
     6721 ggtacatcct gacctcgaaa aacgattcca cattgctcta acctttcgag gtataattta
     6781 gcttcctcta aagaagcaac acctacaaca atatcaataa taggctttgc gctaatactg
     6841 ggaatagaag tgcttccaat gtgttgaata tcaatagcag tcttatccca tatacttttt
     6901 aataatataa tgacatcttt tgcactttta tcccatatca tctgatgttc caataattta
     6961 accataccac attttaaacc tatcactata taccacctca atataatcca cctttatata
     7021 tattacaatt ttttctatct tgataaatag tgatttagct atttcacttt ttcttgacac
     7081 tcgctacatt ccctatcatg aatagatata attcctccac atttagaaca agtatatctc
     7141 tccttctgct tttgcataaa cttctctaaa ccgtactgct gaacaaattt actattttcc
     7201 ataaggcttg cttgatatct cttgttataa cttttttcaa gattttttat gagtttacaa
     7261 ggaaaattga aacattcaaa acaataaaac aacccttttt gacagacaca atcctttatt
     7321 ttgcacttac ggcagtgttc tggctttcct gtatcgccgt tcaaacaacc agcacatggc
     7381 tttttgtgat aacaatgctt atagcaaacc atacaattca ttccacaagg agcaaacata
     7441 atcgtatcaa tactctcttt tggcattttc ataacatttt actcctttca aaaatccaat
     7501 ttgtcgaatt gttatatatt catattgtaa cataaaaaat agctaaaaaa ttcatgcact
     7561 ttcattgcca ataaattttc aaggaaatat gaaagtttaa aactaagaat aaaaagagat
     7621 aaaaaggaag ctactaatca aaatattcaa acacaagtaa tcaatataaa taacttaagg
     7681 tatatttaaa agtatatgat attaccagca gttttaaata caaatataac cacacatgcc
     7741 tcaaaaacac ttattacagt atcttagaaa cttagacaat acataagatt cattaataaa
     7801 aaagttctca atatagatat atttaaccat gttttgaata gtagataatc atatgattaa
     7861 aaattaaata tgacatagcg tttgtggaat ataacaccta aattatgtta taataataaa
     7921 gcaggagtgt gatagtgatg gatgaaaaaa tattagagtt attacaggaa atgcaaggta
     7981 atattagtaa tatgcaaggt agtatcagtg gtatcgaagg cactattagt gatatgcaaa
     8041 gcaatattaa tggtatcgaa gacactatta gtggcatgca aggcactatt agcgatatgc
     8101 aaggcagtat cagtagtatg aatggcacta ttagcgatat gcaaggcagt atcagtagta
     8161 tggatggcac tattaatagt atgcaagaca gtattactga tatgcaagtt gaaattaaag
     8221 aagtcaatag taggttagac aatatggaac ctgatattaa agacttaaaa actggacaag
     8281 ttgaaactag tagtagactt gatactattc agagtgatgt tacagaaatt aagtcagatg
     8341 taaaaattaa cagaaataca ttagatacag tgattaaaca tacctcttca ttagtaaaag
     8401 atacatcaga tattaaaaat gatttaaata atatagagat agtcacatct aagaattcga
     8461 atgatattgc taaattaaaa tcagttaaat aatatactat agtaaaaaat acctgaaaat
     8521 ttcacatact tctttaaagt tataccaaat tttatacaga aatactgatt atccgacaac
     8581 acactttact tataaataat ttattaaaaa aatactcatt ttatcctaaa attaacacga
     8641 ctaaatgagt cttgatataa taataaaaaa taagggagat tgaacactaa aaagttgtgt
     8701 atctccctta tattctataa tatagttata taatttttca gcctaaaata aaagtccatc
     8761 tttgattaaa acacatcccc ttatcactta taaaaggata aaatcttgtt ttcatttttc
     8821 catttatatc ttcttgctta agagtaataa ttttttcttt tgatacttta agtgattggt
     8881 taaatttaac caaacgacta agttttgttc aacatctatt tttcatattt tccaatactt
     8941 aatctatttt ttataaataa ttcttttatt tctaactcac ttacattttt tacataaaac
     9001 gaagcatata aatttcaacc ttatcttgta tggttcttaa tacttaatga gtagcttaag
     9061 tatcaatcat tataaatttt tcccttacat ttaacactct taccatttcc aaaatataca
     9121 aaatatattt tgaacatttg tacaatgatg aaatacaata catgaaaata caatatcata
     9181 actgttgtct aaaaatagaa gttcttcaac ataacctttc acaaaaaaca tattatctaa
     9241 gtattttttg tctgctcctt gttttcctgt ttgtagcata taaattactg catctaaaca
     9301 tataactact cacacgaaaa aagaaaataa acttccaaaa tacaagctct aaaaactacc
     9361 tctaaagttg tataatgttt actaggaata aggttgtata acatatagtt taaataatca
     9421 ttttttaaaa ttgcaagtta ttgctttcaa aattagatgt ttttatatca aataaatgat
     9481 taagaatttc taaatttaat ggctttataa tgcactccct ttctttggga taaaataatg
     9541 ttgcacagat actttttcta aagataacaa ttttattttt ctatcaattc caccagcata
     9601 cccagtcaaa ctcccatttg ttcctacaac acgatgacat ggaataataa tagagattcc
     9661 attatgccct actgcaccac cgattgcttg tgctgacatt ttagatattc ctttttgttg
     9721 agcaacttta tatgcaattt ctccataagt gattgtttta ccatatggta tttgttgtaa
     9781 aattttccac actgtagtct gaaaaggagt acctaccata tggagtgatg gtgtaaaatt
     9841 gggttctttt cccgaaaaat aaatatctaa ccatttcttt gtctgctcga aaattggcaa
     9901 ttctttttct tgatgttctt ggtcaagatt ataagcataa tacttttgat tctcaaacca
     9961 aagaccagtt aacccaatat tatctgatgc taaaagaata tttccaattg gactatggta
    10021 atagctaata tattgcatac aattcctcct tatattctga aaacttcact caaatcatta
    10081 gacacttctt ttctatctac cattatgtaa aaacatacta atataatttc cttctaacta
    10141 cactttcgtt ataatatcta tcctcatatc atattagttt tatatttacc tgcatctgtt
    10201 catccttttt tccctttccc tttgatgaat ataaaattca tacatttaag cttcctactg
    10261 tattaacttt tatagaagtg tatattataa ttttatcact aaataggcca aaaaaatatc
    10321 gatatagcat caagtaatat cataatactt tcaaatggga aaagtaagaa taggctgtaa
    10381 aaaagaaaag tatttttatg ttagaaatcc agtaaaatca ttagtagata gaataacaga
    10441 aaacgaacaa tttgctattt atcattttac acataaatat aattgacgca ataaagtgtt
    10501 ttctattcta caacgattaa taaggttaga aaaaagtaaa aatgaactgt atatgtatga
    10561 agcattagtt tctgtttctt tcctttggct tattatgata aaaaatattg ttttgctacc
    10621 aataaaacac aaaaatataa ttaatatcca tatgcaaaaa attttacgtt atattgaaaa
    10681 atactattgt tttacattat attgaagggt actattgtga aaacttaatt ttggagtact
    10741 atcaagcagt ggtaatatca ataaatcaga gtgctccaga tgatttgaac taagtttaaa
    10801 tactatacct tataaatatt ttactgagtt ttgtttatta aaagcaactc aatttctaaa
    10861 aggcacaaat gagctaataa gtgacattgc tataaaagtt gtttttcatc aaataatcta
    10921 ttttagtaaa tgttttaaag aaaaaacggg atattctcca aaagtgtaca gaaatataaa
    10981 aaaagagata tgatttttta tttcttttga ttatccaaag agtaaataaa aaatattgta
    11041 aaaaacttac taacaacggc aatgctttga actatggtaa tactttgaac ttttgattgg
    11101 actttttttg ctaaaataat atatttaaat agcaaagtaa tctgtttaat caataaaaaa
    11161 taagagataa aagaaatcaa ctaaaacttt tacctcttat tttgatagtt tatcagagct
    11221 aatatttaca ctctttttat ttttgatact tcattaaaaa tgatgcttac tctccattca
    11281 cttttaattc ttcattaaaa atgatgctta ttctccattc acttttaatt cttcattaaa
    11341 aatgatgctt actctccatt cgcttttaat tcttcattaa aaatgatgct tattctccat
    11401 tcacttttaa ttcttcatta aaaatgatgc ttactctcca ttcgctttta attcttcatt
    11461 agaactatgc ttactctcca ttcactttta attcttcatt aaaaatgatg cttactctct
    11521 gttcactttt aattcttcat taaaaatgat gcttattctc cattcacttt taattcttca
    11581 ttaaaaatga tgcttattct ccattcactt ttgattcttc attaaaaatg atgcttattc
    11641 tccattcact tttaattctt cattaaaaat gatgcttatt ctccattcac ttttgattct
    11701 tcattaaaaa tgatgcttat tctctgttca cttttaattc tttattaaaa ctgatgctta
    11761 ttctccattc actttcaatt cttcattaaa actgatgctt gcactctgta aactcataaa
    11821 catccattaa tgcttcacca aatctttgat agtgaactac ttccctctca cccaaaaatc
    11881 tcaatatatc tttcaaatca gggtcatcag ttaaatttat taattgatag tacgtagcca
    11941 atgctttttg ttcagcagcc atatcctcat gtaaatcagt taccgaatcg ccaaagacat
    12001 taatataact tgtagtaaag tttactccat tagaatctac tggctgaaga ccatgtccaa
    12061 aattagcata attactacca agtccagcag ccttaagctc ttctggagta caattgccta
    12121 ttaattgata taacactgaa gaaattatct caacatgagc catttcctct gtacctatat
    12181 cagttaaaag tgcacgagat tttccagttg gcatagtata tctttgttga agatatctag
    12241 ttgcagctgc caactcacca ttaggtcctc ccaactgagt catgatatat tttgccattc
    12301 tagggtctgc ttgtcttatg ttaactggat gttccagtgt tttttgataa atccacatat
    12361 tatcttactc ctccttaata tatatttaat aataaatcta attttaaagt ttaataattt
    12421 tatttataaa atgatttgtc ccatggccaa ggttcagata tccatgacca aggatattca
    12481 cttgattcat atccaaaatt agttaatgct ccatatttag attcataatc acacacagct
    12541 tgtgtaaatt tatcgcagta tctgttgtat gaatcaagag ctttcttatc ccttggattg
    12601 ttatctaaat ataaatttaa ttctatacag gcaaattgat attctgaaat tctctctaaa
    12661 agttctcttc tatttaatga cattagtaca tgttaccccc ttttttagct ctaactctat
    12721 ttttaggatt tgcatatagc caatcattat agttatttga ctctattaaa tctaattcag
    12781 gaaataaagt acctcttttt aatgctgttt ttggattgta tgtgtcatca taaatttgac
    12841 gattgatgta tggtctagca aattcacaac aatagtcttg tttatcctct tttaaatatc
    12901 ccattaatta tcctccttaa tttttagaat tttaaaatag cattattttt aaaaattgta
    12961 tactggttct atattataac tggt
//
LOCUS       DAYAOY010000114        12953 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYAOY010000114 DAYAOY010000000
VERSION     DAYAOY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.114"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            133..870
                     /gene="cas6"
                     /locus_tag="ACGI6B_003379"
     CDS             133..870
                     /gene="cas6"
                     /locus_tag="ACGI6B_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFG9752792.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            875..2350
                     /locus_tag="ACGI6B_003380"
     CDS             875..2350
                     /locus_tag="ACGI6B_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752793.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2370..3383
                     /locus_tag="ACGI6B_003381"
     CDS             2370..3383
                     /locus_tag="ACGI6B_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HFG9752794.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3399..4196
                     /gene="cas5"
                     /locus_tag="ACGI6B_003382"
     CDS             3399..4196
                     /gene="cas5"
                     /locus_tag="ACGI6B_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HFG9752795.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4223..6631
                     /locus_tag="ACGI6B_003383"
     CDS             4223..6631
                     /locus_tag="ACGI6B_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFG9752796.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7072..8019
                     /gene="prmA"
                     /locus_tag="ACGI6B_003384"
     CDS             7072..8019
                     /gene="prmA"
                     /locus_tag="ACGI6B_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFG9752797.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8038..8796
                     /locus_tag="ACGI6B_003385"
     CDS             8038..8796
                     /locus_tag="ACGI6B_003385"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFG9752798.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8798..10096
                     /gene="mtaB"
                     /locus_tag="ACGI6B_003386"
     CDS             8798..10096
                     /gene="mtaB"
                     /locus_tag="ACGI6B_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HFG9752799.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10139..10489
                     /locus_tag="ACGI6B_003387"
     CDS             10139..10489
                     /locus_tag="ACGI6B_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFG9752800.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10652..10831
                     /gene="rpsU"
                     /locus_tag="ACGI6B_003388"
     CDS             10652..10831
                     /gene="rpsU"
                     /locus_tag="ACGI6B_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFG9752801.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10861..11304
                     /locus_tag="ACGI6B_003389"
     CDS             10861..11304
                     /locus_tag="ACGI6B_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFG9752802.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11415..11912
                     /locus_tag="ACGI6B_003390"
     CDS             11415..11912
                     /locus_tag="ACGI6B_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFG9752803.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12105..12668
                     /gene="raiA"
                     /locus_tag="ACGI6B_003391"
     CDS             12105..12668
                     /gene="raiA"
                     /locus_tag="ACGI6B_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFG9752804.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
BASE COUNT         5494 a         1146 c         2340 g         3973 t
ORIGIN      
        1 aacagtagaa tatatttaaa aaattaaaaa acaaaattaa atttattatc aaaaaatcat
       61 tttatgatat gttttatttt tatattacaa atagagaata atgaattata ctagaaagta
      121 ggaggtgaaa atatgagcag aatttcaatt tttatggaat catgtaatgg caaatttcca
      181 aatgaaaatt caatgttgtc agtttccttt ataaaaaata ttttaaatgc agaaaacaaa
      241 gattttgcta aaaacatatt taactatggg gaagaaagaa aaaacaataa gcaaataaag
      301 gatataaata cagccattta cataccaaat cttataagaa gcaaaaatga gttacttgtt
      361 gatggagata taatattcaa tataagtttt tataattatt ctatgttcag taaattatac
      421 aatggaattt taaaagtaaa ggagctagaa tataaaggtt atagatttaa aataaaaaat
      481 ataaaaatac ataaagaaca cgagataaaa gaaaatggtg ttatatttaa aactatgtct
      541 cctataatag taaagaataa agaaggtaaa tatttagatg tagaagattc aaattatata
      601 gaatgtttaa actatatagc taatctaact ctggagagta taagaggtag tgggcttaga
      661 aagccattgg aatttatacc tattaacttc aaaaaaagag tgttaaaaga aaagattcgt
      721 ggatttaaag tgagagaata ttactatata aatgcttatg caggaacatt ttttttaaaa
      781 ggagatatgg aagatttaaa tgcactgtat aaaatgggaa ttggatatag aaggacagaa
      841 aatgcaggaa tggttgatat attaaagtag cgaggtgaga gaaataaaag ctaaatttta
      901 tagatcaggt gatattgtaa gagatattgg cattgtttac ttttatgaat tactaatgga
      961 tttaaaaaac gaactagaaa aaaatgatta taagctaaaa tttacttgtg agttaaatag
     1021 aaattattta tctttagaat ctgcagaata tatagaccct aagtatatat ctgattatat
     1081 attagaaaat cgacttttta aggtatttaa aaatgggaaa aaggaaacct tagaaaaaat
     1141 atttagtaat atagataatt taacgtatgt aaattttatt gaagaattag ataaatcttc
     1201 tgcaactgct aaacaaaagg aagatattaa aaaagatttt aaaaacagga ggtttccata
     1261 tgttagaaat agtggtaagt atggattaaa cacgagttta gaaaatatgg aaacaaatgt
     1321 aaaaagaata gtagatactg taattaaatc aaatatagag atagatgaaa tagataaact
     1381 tgatttgaca caatatgaag aaaaagatag tgtatgtaat gtttgcaata taaataaaac
     1441 aacaaaacta gatattgatt taagagaacg aaaggactct aagtataact ttttatttag
     1501 aggtgcagaa aaatcaggct ttaaaagaag tggacaagtt gaaagtaata tttgttttga
     1561 gtgtgaattt ttcaacttaa tgtgtctttt atatataaat ttaaaaagac caatggtttt
     1621 tgcatatacg aatgatttaa gagaattagc atttttaaat cataagataa tgcttaaaag
     1681 aaaaatgtat tcagataaat cattttataa aaaacttttg catgaaaaaa taagttcaat
     1741 tagactatat agctttgata ttgatacgaa taaaggtata attctaaagt ttgattctat
     1801 tatagagtat aaagaattat taaaaaaaat tgaacttata gatattatag ataattataa
     1861 tttttcaaga gagccaggta atactagaaa tttgggtaaa agaatgattg ataatgggaa
     1921 tttatcaaat ttaaaagaat tattacttga taatataagt gttttaaagc aatcaactgg
     1981 aacttctcga gaaatggatt ttgtatctag ctcatataat atcggtttat atataaaatt
     2041 gtgttggatt gtagatggag gggaggagta taaaatgaaa aactatatgg aaagtaattt
     2101 aatatattca agagtaggta aagacttatt taataaaatg acagatgaat ccagaagaaa
     2161 aaatttttct atgaaagtga ttcaactact taagtctaat gatagaactc agctatttca
     2221 aactataatg catgtgttag tttctaatgg aatcttaata ggagaagggt ttgtagaagg
     2281 tctaatgcag agttctgaat tagaattaaa tacgaatgta ggtctattta ttcaagaaat
     2341 catgaaataa aaaaggggga aaatttaaaa tgaatgaaaa aagagtaaaa ggagtaacat
     2401 taactatact gacagaaagc ccagttccac tttcatatga ccaagggtat ggaaattata
     2461 caccaataaa aaaagaacaa tatagagaaa aaatacatgc aaaaactagt atagccacaa
     2521 taacatatga tttaagaaga atgctccacc aagaatatgg ttggaattta agtaatattg
     2581 tttttggaaa aaaaggaaat atatatccat ctattaagaa aaatgtagga tgtgtagatg
     2641 aaaatggatt agagactgat gtatttggtt atttaatacc acttaaagat aaaggttcta
     2701 taagtaaagc ttctccactt agaataatac cttttaggtc attaaatcca tataaaggtt
     2761 caacacagct aataacaaat agaggctttt taagtagtga atttggtaga aaatattatg
     2821 atgagaagga agaaaatgaa gtacctagag atgaaaactt tccaacatca caagcattag
     2881 caatagaaga gactttatca gattactacg tttatacaat aacattggag cttgatagaa
     2941 taggagtagt agaagtagaa gatggaaagt tacttttacc agaagaaaga aaatttatga
     3001 gtaaggagtt aagagaaaaa gcagtaaaag atatattaga tgcaattact gtgtttacaa
     3061 gaaacataaa acattcatca gtactattaa aaccacttgc agtaataggt ggagcttttg
     3121 ataaagttgt accatttttt tgggatgatg tagattataa tgctgatagt ggtgagataa
     3181 atttagaggg agtcatagaa actatagaaa gttatagcct taaagaaagt aacacaatac
     3241 ttgctatcaa tgatagatta aaaatttcta ataaaaagga attaaataag tttaatttaa
     3301 gtaaataccc tgtaaaagag attaaaaatc tagcagatag attggaaatt ggagaagata
     3361 atatgtggta tttaaaagag taggaggttt tttgaattat gaaagtatta caatgtaaac
     3421 ttaagcaacc aactgctcac tatagagacc caaaagtatt tcaaaatgaa tatataagta
     3481 ctttaaattt accatctaag accacaataa tgggaatgat aacgtattta tgtgatagaa
     3541 gattaaactc tgatatagat ataggaataa ttggaacaca tcatcataga gaactagaat
     3601 tttcaagagg agaaaacatt gatttttgga atgaatatat aaaaatggga aaaggtaaag
     3661 ataaagaaaa gtttttgctt aaaggaaact actatgatta ctataaagag cataaagctc
     3721 aaaatagcat attaaactat gaagttctta aagaagtaga actgactata tttatttctt
     3781 gcaaggatga tgaagaactt gaatttatta gaaaaaagct tgaaagtcca tgtaaatatg
     3841 caaatttagg aagaaaagaa gattttgtaa taccaagtga aaaagggtgt tttgtgaaag
     3901 aagttaagtt aaaagaggtt atacctatga atactcgtga tgctataaaa gaaaatataa
     3961 aattaaagaa tacatatgta agagttgact taagagataa agagaatgtc gagactataa
     4021 taaatcaagg tgtattgatt gcattgcctc ataaatataa agatttagaa gcaaatagag
     4081 atgatagagt ttatgagttt tgccattata tatatgttga taatgatggt atatatccta
     4141 aaaatattaa ggtaaatgta tgtattgata caaaagaagt ttttacatgg ctttaaatta
     4201 ttttgaaaga taggtgttta tcatgaaaaa tatcatgcaa aaagaaatat tagctaagtc
     4261 atacaattta agaactaatc aacaagaaca aacattagtt gaacatatta aggatttatt
     4321 tgaagtatta gaatctatat tagaattaaa tctatattca gataaagatg tagagatact
     4381 aaaaatatgt tgtgctttgc acgatttagg aaaaattaat tcaattatgc aacaaaaaat
     4441 ggagattaat aataaaatat cgtatagttg ctcagaagaa gagaggaaaa agttagagtc
     4501 tgacaaaaaa tctttaaaaa agatagcaag acataatata ttttctggag catttttaaa
     4561 agatatatta gaaaaaatga acttgtcaga agaagataag ttatatatat ataaaagtat
     4621 aatgttacat catggaaact atgaagatta tatgaggtta agtattagta aagttcaaga
     4681 agaaatttat gagtatattg aaaaaggcat tttagaaaaa gaagagttta atctgaaaga
     4741 tatagaaagt tatataaata gcattttaaa tgtagacttt aaatttggtg aagatgtatt
     4801 ggattatgat tacatagata aattaagtga aagtatgttt atagacagtg attataacaa
     4861 tggtcaaata gatgattcag ttttaaatta tagaaagttt aagtatattt tatataaagg
     4921 gatgctgaac ttaatagacc atagtgcatc aagtagacag aaaggtataa aattttataa
     4981 cgattttact gatgaagaaa ttgataatat gattttaaat gagatatata agagtcaggg
     5041 taatttggaa aaaaataata tagagttcaa tactatacaa aaaaggttga gagaactttc
     5101 tggaagaaat gttctgactg ttgcatttac aggttcagga aaaacagctg cagattatag
     5161 aaagactttt aaaagaaaat tctttttagt gccaaataaa atatcagcag aaagttttta
     5221 tagaaaaaat atttttcaaa ataaaaatga tttagataca agaagtagta atgattacat
     5281 tggattactt catggagata taaacttata ttcagagaat gaagataatg gagaacatga
     5341 ttttgtattg acattaaggg atatagattt gagtataaac ttttgcaaac cttgtgtaat
     5401 tgcaacagtt gatcagttat tacttagtat gtttaaattt ccgggatatg aaaaaatatt
     5461 cgcagccgta aaaaatgcat ctataactgt tgatgaagta catttattgg aaccaaagat
     5521 gtttttgatt ttgatatatt ttatgcagtt tgcatgcaag tacttagatg ttgattttca
     5581 cttaatgaca gctacacttc caaaatcata taaagagcaa atgataaaca agggaattat
     5641 ttttcaagaa gaaagtaatg aaaatgttac tgataaagga gaaatagttt ttattgagag
     5701 caacaaggaa gaagagattt gtgaaggtaa agatgtaaag gtttctttta taaaggaaaa
     5761 agaaatcaag tcaatagttg aagatgcttt agaaaataaa caaaaaattc ttataataaa
     5821 gaatactatt gattcagtca atagaacata tgagttttta aaagaaaatt tatctgataa
     5881 gtatggtggt gtagatatag atgttttaca tagtagattt aagttcaaag ataaaaaaga
     5941 gaaatatagt aaaatattaa atggagaggg tgatatttgg atatcaactc aatcagtaga
     6001 aatatccttg gatttggatt tcaatataat aataagtgat ttagcaacta tggatagctt
     6061 aatacaaaga atgggtagat gcaatagaaa taataagtat gaatatggaa acttttatat
     6121 tctacctagt gaagataaaa tttatgacaa caaacttaag agcacaacta aaaacatctt
     6181 aaaggacata ttaaaaacta actctatttt tacaatggga attagaaaaa ctattttgga
     6241 tgattactat gataatagtg tagtcaaaaa atattttgaa gagaatttta taagttgtga
     6301 taaagaaatt aagaatattt atggtgtaaa taaagaaatt tttgatggat tggatttaat
     6361 ttttaatttt gaaccttaca aaaatattgt tgatagtaag aaggaagcag ctaaaatttt
     6421 tagagatgtt gatgttagtt acaaaattat tctggaagaa gatttttata aggaagatag
     6481 agagttacaa caagattcga ttcaagtatc agggtttata tttaatagat tatattatta
     6541 tgggttaata agcaaagtag aaggatacat ggtactaaaa tctagtagta agtttgagta
     6601 taatagtact gtaggcttaa ttttgaagtg agcatatgtt taaatataat atacctttag
     6661 acattgaaaa tacaaaaagt actactgtaa gctcactgta aaaactattt tttaaaagga
     6721 aaaataagtt gtgagataga gtgtttacaa gtgtatatag ataataaatt tgaggtttaa
     6781 gattaactaa gtgggtttca ggtacatcgc gaaaataatt ataagttgga gattttgtat
     6841 gtttaagatt aactaagtgg atttcaaata ttgatatagt agatataata gaagttttat
     6901 tattaggttt aaaattaact aatcggattt ttaaatattc tgactattat ataaggatat
     6961 tattagattg aatttaaaac caataatata aaacaacaaa atattatatt tatgatataa
     7021 taaaagttat ggaaaaacag gaatattact atagagaaga gaggtaaact tatgaataat
     7081 tggatagaag taactataaa aacaacaaca gaagcagttg aacctataac gaatatacta
     7141 tatgaacaag gagctggagg agctgtaata gaagacccaa aagacttttt attccaaaag
     7201 aaaaatgaac tagactggga ttatgttgaa gaagaagtat ttaaaaagaa tgaagaagat
     7261 gatgttctta taaaaactta tgtttctgaa gaaaaaaatg tgatggaatt tgtcgaaata
     7321 ataaagcaaa aagttttagg attaaaagat tttggtatag atataggtga aggaagtgta
     7381 tcattggacc aagtaaatga agctgattgg gcaaatgcat ggaaagcata ctacaaacca
     7441 acaaaagtag gacaaagggt agtagtaaag cctacatggg aagattatgc tatgcaagag
     7501 ggggatttaa tcatagagct tgaccctgga atggcatttg gtacaggtac acatgagact
     7561 actagtatgt gcattagaga attagaaaag tatgtgaata aagattctaa agtatttgat
     7621 attggatgtg gtagtggaat ccttgcaata gcagcagcta aattaggagc aaaagaagtt
     7681 gtagctgtag atttagatga agtggctgta aaagttgcaa aagaaaatgt tcttgaaaat
     7741 aaagttgaaa aaagtgtatc tgtaatgcat ggtaatttaa ctgatgtaat aaaagataaa
     7801 gcggatgtaa tagttgcaaa tataatagct gatataataa agatacttgc aaaagatgtt
     7861 caaaacttta tgaaagaaga tgctatattc atatcttcag gtataatttt ggataaagta
     7921 gaagaagtta aagaaagctt aattgaaaat ggatttgaaa tagttgaagt tcaaaaatta
     7981 ggtgaatggt cagcaatagt ttctaagctt aaaaaataag ggatatggtg acgtataatg
     8041 gatagatttt ttgttgaaaa aaataatata aatctacaag ataaaacttg cactatagaa
     8101 ggtgaagatg taaaacatat ttcaaaagtt ttaagatgca aattaggtga gaaacttgaa
     8161 atatgtgaca agaataataa tgagtatatt tgtgagataa tgaatataga taagagtata
     8221 gttaatttag aaattttaga aaaagttgat ataaatagag aatcagaact taaagtaagg
     8281 ctataccagg gacttccaaa agctccaaaa atggaaataa tacttcaaaa attaactgaa
     8341 gtgggtgtag aagaaataat attagttcaa actaagagaa gtgttgttaa agttgatgac
     8401 aaaaaagaag ataaaaaatt tgaacgttgg gaaagaataa tttatgaagc agcaaagcaa
     8461 agcaaaagag gtaagatacc aaaattaaga ggtgttttaa gttttaaaga agctttagaa
     8521 gatatgaaaa aaaatagtgt aaatatttgc ccttatgaaa atgaaagaac agtttcaata
     8581 aaacatgcac taaaaaaatg tgattctaat atagatagtg ttggaatttt tataggtcca
     8641 gaaggtggat tttctgaaga agaaatagaa caaatacaaa aaaataattg taatgttgta
     8701 tcattggggc ctagaatatt aagaactgag acagcttctg ttgtagcatc aacaatagct
     8761 ttgtatgaac taagtgattt aggaggagaa aaataaattg aaaaaagtag ctttttatac
     8821 acttgggtgt aaagttaatc aatatgaaac agaagctatg ctagaactat ttgagaaaga
     8881 tggttatgaa caagtcaata gtgaagagta tgctgatgtt tatgttataa atacatgtac
     8941 agttactcat atgagtgata gaaaatctcg tcaatatata agacgagtta aaaagaagaa
     9001 tccagatgca ataatagccg ttgtaggatg ttattctcag gtatctccgg aagaaatttt
     9061 ggatatagaa gaagtaaatt tagtcatggg tacaaatgat agaagaaaga ttgttgaaga
     9121 aattaaaaag ataaattcaa gtaagaaagt tagtacagtt gatgacataa tgaaagttaa
     9181 agcttttgaa gaaatagaaa tcagccaaac taatggaaag actagagcat ttatgaaaat
     9241 acaagatgga tgtgacagat tttgtactta ttgtatcatt ccttatgccc gaggacgcgt
     9301 tagaagtaga gatattgata gtatagtcga tgaagttaaa aaattggcta ataatggcta
     9361 taaagaggtt gtattaacag gaatacatgt tgcctcttat ggaaaagatt taaaggatag
     9421 ggatattaaa cttcttgatg taataaaaca aataaaccag atagaaaaaa tagaaagaat
     9481 taggcttagt tctgttgaac caattttatt tacagatgaa tttgtaaatg aagttttaaa
     9541 gatggataaa gtttgtcctc attaccattt atcacttcaa agtggatgtg atgaaactct
     9601 aaaaagaatg aatagacgat acacaacttt agagtacaaa actattgtag atagattgag
     9661 aagtaaaatg ccagatgtgg caataactac agatgtaata gtaggttttc ctggagagac
     9721 aaatgaagag tttaaaaaaa cgtacgagtt tttaaaagaa atagagcttt cacaaatgca
     9781 tatttttaaa tattcaccaa gaaaaggaac tccagctgca actatggaaa atcaagttga
     9841 ccctcaaatg aaacacttta gaagtgaaca acttttaaat ttaagtaaag taaattttaa
     9901 taaatttgca actaaattta tcggccgtga gttggatgtt ttatttgaac aaaatataga
     9961 aggcaataaa tatgagggat taacttcaaa ttatataaga gttgttgttg aaagtgacaa
    10021 aaatatacaa ggtcagatat taaaagtgaa aataaatgat gtaaaagatg aatatgtaga
    10081 aggaattttg ctataacatg tagaaattat aatatttata agataggagg tggaaagaat
    10141 ggattgtatt ttttgtaaaa tagcgaacgg agaaatacct tcaactaaag tttatgaaga
    10201 tgatagagtc ttagctttta atgacttgaa cccagttgct ccatatcata tattagttgt
    10261 acctaaaaaa cattatgata gtttaattga tattcctgat aaagagatgg acattgtgtc
    10321 acatattcat gttgttatta ataaaatagc taaagagaaa ggttttgacc aaactggatt
    10381 cagagtcata aataattgtg gaagtgatgg agggcaagaa gttaaacatt tgcactatca
    10441 tattttagct ggtaaaaagc ttcctaatta cgaagcggga caaaactaaa ctaaattatt
    10501 gaaaaattga gttccttctt gataaaaata cttgataaaa gtaaaaattt agtttataat
    10561 aaacaaagtg ttaggttgta atgcatatta tgcaaaaaga acatagttac agtcccagca
    10621 taattgcgct attggaggga gggaaaaaga aatgtcagaa gtaagagtta gagagaatga
    10681 aacattagac agcgctttaa gaagattcaa gcgtcaatgt gcaatgtctg gcatcatgtc
    10741 agaagtgaga aaaagagagc actatgataa gccaagcgtt aagcgtaaga aaaaagctga
    10801 agcagcaaga agaaaaaacg ctaagaaata gtagtaaata agaataaaga ggtgaagggg
    10861 atgtccctta agcaaaagtt acaagaggat ttgaaatctt cgatgaaaaa caaagataca
    10921 gtgagaaaat ctgtagtaac tttaataaga gcttctataa agcagtatga ggttgataat
    10981 agggtcgagc ttgatgaaga tggaattatc gatgtaatag caaaacaatt aaagcaacgt
    11041 agagatgcat tagttgaatt tgaaaaagct ggaagagaag acttaataaa agaaacagaa
    11101 ggtgaaattg aagttttaaa agagtaccta cctcaacaat taagcgaaga agagttagaa
    11161 gaaattgtaa aatctactat atccgaagta ggggcaactt ctatgaagga tatgggaaaa
    11221 ataatgtcag ttatccaacc aaaagttaaa ggaagagctg acggaaaact tataaataag
    11281 ttggtaaaac aaaacttaca atagattgat atagcctaga gagaaatctc taggcttttt
    11341 atagtttttg ggaataacga gtcatatttg agcatatcta taatcaaaga aaattttgaa
    11401 agaaggggaa aaatttgatt atatcagtaa aagagattag gaattttttg ataaacatat
    11461 ttataccact aataataggt tacttaagta gcatcttaac aatgattatc agtggaacag
    11521 atatttcaac ctattattta caactcacaa agccaagctt tgcaccacca gcttttatct
    11581 ttcctatagt ttggactata ttgtacatat tgatgggtat atcttcatac ctaatattaa
    11641 gaaagggtta caacttacct aaagtaagag atgcaatttt ttattatgga ttacaacttg
    11701 tattaaactt tatttggagt atactatttt ttggatttgg acttagattt acagctttga
    11761 tagatataat aataatgatt ttggtttcta taataatgat ttcgaagttt tctaaaatag
    11821 ataagagatc tggatacata aatttgattt atttaatgtg gcttgtttat gcaggatttt
    11881 tgaactattt tatatggttt attaataaat agaatgtaaa gattttgtaa attaccctgt
    11941 acttttaata atatcataac aaacgaaaaa taaatagact attaaaaata atcattttca
    12001 tatacaataa tattaattta aatacaataa atttataaaa attgtattta aagattaaca
    12061 tattaaagta ttagaattgg aaaggcttga agggagaggt ttttatgcaa ataatagtat
    12121 ctgggagaca aatgaaatta actgacggaa taaaaggata cgtagatgga aaattaagta
    12181 ggttagaaaa ataccttgat cctgaatcag aagtgaaagt aacagtaagt gcgaaaaagg
    12241 atagacaaaa agtggaggtt acaataattc ctattaacgg tcaaataata agagcagaag
    12301 atgtagaaga tgacttatat gcagcaattg atattgtttg tgacaaatta agtagacaag
    12361 ttgtcaaata taaaactaaa gttaaagaca aagttcaaaa taataagagc ataagatttg
    12421 aaaatcttga ctttatagat aatagcagtg aatttgatga ttatgattat gatgatgaag
    12481 aagatgaaaa tatagtaata gaaagaagaa agaaatttaa tgtaaaacct atgagttctg
    12541 aggaggcgat acttcaaatg gaactagtag gacacaattt ctatatgttt agaaaccaag
    12601 ataattttga aataaacata gtgtataagc gtaaagctgg tggatatggt ttaatagaac
    12661 aagattaata aaaattgtat acgtttagat tttatataag aaaaaataaa aaggtaatca
    12721 tgttgatttt ttatcaatat gattaccttt ttcttgttta aataatatat tagatattag
    12781 gtatttatta tataaaaatt ttaaaactaa tctgttacaa catcaataaa gtgcataaat
    12841 acctatttca agcataaaat taattaaggg ggtaatgctt atgttagaaa tatccactga
    12901 tttacaagta aatcaaccag ttattactgt tacaagtaat acgtttatta gta
//
LOCUS       DAYAOY010000115        12541 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYAOY010000115 DAYAOY010000000
VERSION     DAYAOY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.115"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            75..713
                     /locus_tag="ACGI6B_003392"
     CDS             75..713
                     /locus_tag="ACGI6B_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFG9752805.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            740..1639
                     /locus_tag="ACGI6B_003393"
     CDS             740..1639
                     /locus_tag="ACGI6B_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HFG9752806.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(1746..3410)
                     /locus_tag="ACGI6B_003394"
     CDS             complement(1746..3410)
                     /locus_tag="ACGI6B_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFG9752807.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            3702..4415
                     /locus_tag="ACGI6B_003395"
     CDS             3702..4415
                     /locus_tag="ACGI6B_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HFG9752808.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            4574..5137
                     /locus_tag="ACGI6B_003396"
     CDS             4574..5137
                     /locus_tag="ACGI6B_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752809.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            5534..7234
                     /locus_tag="ACGI6B_003397"
     CDS             5534..7234
                     /locus_tag="ACGI6B_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752810.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            7404..8021
                     /locus_tag="ACGI6B_003398"
     CDS             7404..8021
                     /locus_tag="ACGI6B_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752811.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     regulatory      8132..8300
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            8382..9575
                     /locus_tag="ACGI6B_003399"
     CDS             8382..9575
                     /locus_tag="ACGI6B_003399"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFG9752812.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            9593..10885
                     /gene="lysA"
                     /locus_tag="ACGI6B_003400"
     CDS             9593..10885
                     /gene="lysA"
                     /locus_tag="ACGI6B_003400"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFG9752813.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            11102..11761
                     /locus_tag="ACGI6B_003401"
     CDS             11102..11761
                     /locus_tag="ACGI6B_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9752814.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(11916..>12541)
                     /locus_tag="ACGI6B_003402"
     CDS             complement(11916..>12541)
                     /locus_tag="ACGI6B_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9752815.1"
                     /translation="PKNFLIYTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLY
                     SSTARNKADNIIEFFRTTHDIFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLAS
                     IIFLGNIIPYFGPFIGAIPPIAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQ
                     VGIGAIWVISAILVGQSLFGFIGVFLSVPIAAVVKTYVDKYIDNRLQ"
BASE COUNT         4956 a         1404 c         2112 g         4069 t
ORIGIN      
        1 actaaaataa acttataaag aaaataaagt ttataaataa ttaagtgcat attaaaaata
       61 agggggaagt taaaatggat caaaatttaa attggcttaa tgaaaaaaga atagaaaaaa
      121 caataaaatc tttggaaaac aataatatga aagggtattt agtaagtaac aaagaagagc
      181 ttcttaaaaa aattgaagaa atagttaaag caggttctct agtaggatgt ggaggttcta
      241 tgaccttatt tgaaacagga gtgattgatt ttataagaga aaaatataat tttcttgata
      301 gatatgcaaa agatatcaaa ccagaagaaa tgaaagaaat atatagaaag tcattttctg
      361 cagatgctta ttttacaagt actaatgcga taacagagga tggagaactt tacaatttag
      421 atgggaatgg gaatagagtt gctgctatga tatatggacc tgataaagta atagttatag
      481 caggagtaaa taaaatagtt aaagatgtag atgaagctat aaaaagaaca aaaacaatag
      541 ctgctccagc caattgtaaa agattaagta caaatactgg atgtaagact actggtatat
      601 gtatagactg ttcaagtcca ggaagaatat gctgtagtta tacacttata aaaagacaat
      661 taacaccaga aagaatacat gtgatttttt taaatgaaga ttttggatac taaaagaata
      721 aaatgagtaa ggagaaataa tgaaaaaagt aaaacttata tacaatccta attcaggtga
      781 aaaaaagata ttatcaaatt tagacactat catagaatta tatcaagaaa aaaattattt
      841 attgataccc tatagattaa ctattaaaga acctgtaaag aatgcattta aggaagttga
      901 tagttcttat gaacacatat tgatagctgg tggtgatggt actgtagatt tagtagtaaa
      961 tgcaatgaaa gaattagaca taaaaattcc aattggaatt ttgcctacag gaactgcaaa
     1021 tgatttttca aatgctcttc aaatttcatt tgatataaaa gaagctataa atgaaatcat
     1081 aaattcaaaa ccaaagaaaa tagacatagg gaaagtcaat aataagtact ttataaatgt
     1141 tgcaagtgct ggtatgttta cggatgtatc acaaaggata aacacagact taaaaaattc
     1201 atttggaaga atttcttatt atataaaggg aatagaagaa gcattgtata tgaggaaatt
     1261 taagataaaa gtatcttctg aggaaatgta ttatgatgga gatatgtatc ttatgttgat
     1321 ttttaatggc aaaacagcag gaaatataaa tctagcatat aaggctgaaa tagatgatgg
     1381 atatttggat gtaattattt ttaaaggaat gccaatacca aaatcaatac cagttttgat
     1441 aagtgtgctt agaggtgatt atttagatca atataatgga aatgaaatac tatactttaa
     1501 aactaaaaag gtgcatatag aatgtgaaga ttcacttata acagatattg acggagaaaa
     1561 aggaccagat tttcctctta acatagaatg cataaaagat ggaatagaag tcatgggaat
     1621 ctgtagtgaa aaaagttaat catttaaagt aaatgatgaa gactcaaata aaaaattact
     1681 tctaactaag tttatttata gtagaagtaa tttatatttt aagtcttcat tttttgattg
     1741 taagattatt tacctggttt aaaagaactt tttagaggaa ctattctatt aaatacaaat
     1801 ttttcatcag ttgtatattt attatctata ctaaagaatc catttctaac aaattggaat
     1861 ttatcaaaag gtttagcatc ttttatctgt gtaggttcaa caaaaccttg taatatttcc
     1921 aatgaattag gatttatttg ttttaagaaa tgtttacctt cattttcagg tatatcatca
     1981 agaattaatg gttcaaataa tctaaattca catggtatag ctgatttagc atcaacccaa
     2041 tgtattgtag attttacctt acgtcctgta aagccagagc cactcttagt ttcagggtca
     2101 taagtaccat gtatttcaac tacatttcca ttttcatcct taattacatc agtacatttt
     2161 acaaaataag caccttttaa acggacttca tttcctggga aaaatctaaa atatttctta
     2221 acaggttctt ccataaaatc atcttgttct atatacaatt ctctagagaa aggaactaat
     2281 ctctttcctt gagattcatc tttagcatta ttctctattt caagcatctc aatcttatct
     2341 tctgggtaat tagttataat aagttttaaa ggtcttaaaa ctcccatagt aagaggagcc
     2401 tttggttgta agttttctct taggaaataa tcaagcattt gactatctac cgtagagtta
     2461 acttttgata cccctatttc actacagaaa ttgcgcaatg cttctgcagt atatccctta
     2521 cgtctgattc cggatattgt aggaacacga gggtcatccc aaccatcaac aataccctca
     2581 tctacaagtt gttttaattt ccttttactc atgacagtat tatttatatt aagtctagca
     2641 aattctattt gtctaggtat attttccatc tcacattctt taacaaacca atcataaaga
     2701 ggtctttggt cttcaaactc taaagtacat atagagtgag ttattccttc aattgcatct
     2761 tcaattggat gagcaaaagc atacatagga tatatgcacc atttatctcc tgtattatgg
     2821 tgtgttgaat gagatattct atatataatt gggtctctaa ggtttatatt tggagaagac
     2881 atatctattt tggctcttaa tgttttttca ccatctttaa attcaccatt tttcattctt
     2941 tcaaataaat caaggttttc ttcaatagtt ctgtttctat gaggactttc ttttcctggt
     3001 tcagttagag taccacgata ttctctcatt tcctcttgtg tcaaatcaca tacataagct
     3061 ttaccctttt ttattagtat taaagctctc ttatacattt catcaaagta gttagatgca
     3121 aaatttaact cgttccaatc aaatcctaac cactttacat cttcttttat ggactctaca
     3181 tattcaacat cctcttttaa tgggtttgta tcatcaaatc ttaaattagc ttttccatta
     3241 aactccttag ctaatccaaa attaagacat atactttttg catgaccaat atgtaaatat
     3301 ccgtttggct caggtggaaa acgagttatg atactgtcat gttttcctgt ttctaagtcg
     3361 tttataataa tattctttat aaagttagat gagttcgttt catttgacat attctatacc
     3421 tcctatttaa tttgattata atacagaaca ctacactgta ttgcataata ttaattttaa
     3481 tccaagatta gaaaaaaata aaggtgtaat taagagtatt atgcaaaata ttaactttta
     3541 tactaaaaac atgtttttac agtttattta caaaacatta gattggttga attaattttt
     3601 aaaagactgt attatagtat gtatattgtt aagaatatta tgtgaagtta gttatctaaa
     3661 tttattttaa atctgatgac agatttgtaa aggagacgat tatgggaaag gttgcaaaga
     3721 ttttgttaag tttaattgtt attgttttag tacttgcagg aattgttgta tatatcttga
     3781 caccaaaaga aagatatgat gttagttcac aaaataataa aatcatagat gaagaatatt
     3841 tatcaaaagg ctcatatata aattctatgg aggtagtaaa aaatccactt agaatggttg
     3901 gaaaaatatc tgtttcagag gaagagttta aaaatttaat atatacattg atgcagaagc
     3961 atggaattaa tgaacttgaa aataattttg ttgaaatgaa aaatggtaaa ataaaggtag
     4021 caggaccata taaggtattt ggtttggtaa atagtcaata tgaacttgaa ttgagaccta
     4081 ctttaaagga tggaaactta gttgtaagtc ttgagaatgt gaaaattgga aaatttaaac
     4141 taagtgataa aatgctagaa aaaatattaa gtaactacaa taaaaaagtt ccatttgagg
     4201 ttaatggaaa taagattact ttggaaaaaa gttatcttta cccaataaca cttaaaaata
     4261 tatctataaa aaaaggaaat gttgatttgg atatggaagt agaaatagat ttaaaatcac
     4321 aaataaatgg ggcattagga tatttaaagg actctggaaa ggttcaagat atactaaatc
     4381 atctgattaa tttgaagaaa acatctgttg gttaaaaata tagaaaactt atatttatat
     4441 ttttattata ttatataata ttaaagtacc ttaaaatcaa aatttgtaaa accacattta
     4501 ttatgttaga atatcttatg atattaaatt gtaagtgtat aatattattt agttactaga
     4561 ttggggggaa atgatgaata aagtctatat tgtaacaaca tatacaggaa ctatattatc
     4621 ttacttaata aggaatataa gtaaaaagct gtatacacat gtgtcaatat cattaaatga
     4681 aaatctaaaa ccaatgtatt cttttggaag gctaaatcca aggaatccat ttattggagg
     4741 atttgttgaa gaaaatataa atcaaggatt atatgctata agaaaaaaca ctgtgtgtag
     4801 ggtatattcg ttagaagtag ataatttaca atatgaaaat ctatataaaa atataaaatt
     4861 aattagtgat tatagagaag attactatta cgatacaatg gcattaatat atttactaat
     4921 gaatattcag agacaatctg aatataaata tgtatgttca aattttgttg ctgacatgtt
     4981 agaaaaaagt gagatagaca ttttaaataa acaagcattt gaagtaacgc caaatgattt
     5041 ttataattta tctggcttaa ccttagagta tgaaggttta ttatcagaat ataattctag
     5101 acaacattct tatgtatcaa aaatcgcaaa catttaaaaa cttgatttag agtttttcat
     5161 ataaaaaaat attgctagat aattaggctg aagatagtag tttaacttcg ataggattaa
     5221 gaatttgtaa atcttagtta gaatactttc ttcagttttt aaatttctgc taatatttgt
     5281 atttttataa catttttgga tatatataat acagaatagt gcaaaaatcc cactccttta
     5341 ggtgcgggat ggatagcaca aacagtgtat aaaaagtatt tatacataca cgtataatgt
     5401 taaatatagt tgaataagca gaactataaa tatataaaga tatcttaata tataataaat
     5461 atgataaata tttatttttt gtaaatatat ggatataata gtaataataa cattttttag
     5521 agaggagtat taaatgaaac gatatcttaa aacatatgga gttgcattaa taattgtaat
     5581 aatttatgca tttattaaat tacctgtact aagattagat tttacatctt tattttcagt
     5641 aggaataatt ttctttgttg tagcaggaat actagatatg atgttagata gaaatgaaag
     5701 agcatctaaa atggctaaat ataatttttc aatagcaata gctttgcttg tgtatattgt
     5761 agtagttccc tttataactt ctacacctgt acttcatgca aaatcatata gagaactatt
     5821 gggaaaagta actgaaagta aatttactga tgacattagc cctgtcagtg taaatgacat
     5881 tcgtcttgta gatgaagata tggcaatgaa attaggtgac aaaaagcttg gagaagttcc
     5941 tgcaataggg agtgtatcca aattaggaaa atttcatata caaaatgtag atggagagtt
     6001 gtactgggta gcgcctcttg ttcacagaga cataattaaa tggataacat ctctaagtgg
     6061 tacaagtggt tatgtaaaag tatcagctag taatcctcaa gacgtccaat tagttcaaga
     6121 aatagatgga aagccaataa agatagttta ccaaccagaa gcctacttac atcaggattt
     6181 acaaagacat ttatacatac atggtattgt aaatgttggt atgacagatt tcactcttga
     6241 aataaatgat gaaggaagac cttattgggt tgtaagttta tatgaacata aaattggcta
     6301 tggaggagca aatgcaacag gaatagccac tgttgatgca gaaactggaa aaataaatgt
     6361 ttatgatgtt aaaaacacgc ctaaatgggt ggatagaata caaccacaaa gctttgtaac
     6421 agaccaaatt aaagactggg gtgtttatgt taatggattt ttaaactctg ttatatcaga
     6481 aaagggagtt ttagtaccaa cagaaggtac atcattagtt tatggcaatg ataacaggtc
     6541 ttattggtat acaggtataa catcttcagg tggagatgaa tctacaattg gatttatgtt
     6601 agttgattct agaacaaaag aagctagatt atataaacaa ccaggagcta ctgaaacggc
     6661 tgcaatgaaa tcagctgaag gtaatgttca agaaaaaaat tatgaagcga cgttccctgt
     6721 aatgtataat atattgggtc agccaacata tgtatcatca ttaaaagata aggcgggtct
     6781 tgttaagaga gttgcatttg tatctgttga agattataat gtacttggaa taggtgaaga
     6841 taagaatgaa gcattaagaa actataagga tgcattagaa tctaagggta atgatttaaa
     6901 acttgataat gatttaaaag atgaggtatt agaaggcaca gtgacaagaa taagcccaga
     6961 tgtgcgaggt ggtaacacta attattatgt aactttagat tcaaataaag atataatatt
     7021 tagggctact tcaaaagtat ctagcgaatt gccattgact caggttggtg ataaagtcaa
     7081 aatatcgtat tcaaaagaag aatctggtgt aatagaaatg tcagaatttg ataacctaaa
     7141 tattgcaaac tttgaagata aaaaagcaaa agaaaaaaca ggaactgatg aaaatacaga
     7201 ttccaaagat tctgaaaatc aaggtaatgt ataatttaaa taaaaagatt gataatagta
     7261 tttaacatag tgatagctaa tttttataga atttaattct ataaaaatta gctattttat
     7321 tttaattctt tgtaaattta atgtcataaa attaatataa tattagtaca actttgcaaa
     7381 taatacttca aggagaatgt actatgacga atatggaaaa agagatatta cttttaatta
     7441 gcagggttag atgtgttaca gagtctcaat ttagaaaaat ttatggagac caaaaaagat
     7501 acaataaaaa aaattttaaa aaaactttga gaaaaatgtg tagtgaatat acactaaaaa
     7561 aatatccttg tgatgtggat tattataatt ataaagacct ctcttatgta tactatttaa
     7621 acggtagtaa actttttaag ggagatgatt taataaaggc tctagtagga tcagaattag
     7681 ttgttagaat caatactgca ggttgtgatg taaaaagatt ttaccgaaat gttagtgtag
     7741 atgatgtgaa atacgattta tttattgaat atacagacca atttggtaat ataaagcaag
     7801 ttttagttga tgttgactta aataatgaaa tagacatttc taaatacgaa aatctttcta
     7861 aaaaaataga taaatctaca attccatttt ttaaagtacc aaacgttttg gtagttacac
     7921 cagataatct atccgaatta aatgatttag agagatatga taatgagtat agcataaatt
     7981 ttatagattt aggattaaat aaactattta attgtatata agctaacttt agaaaagaaa
     8041 actatcgatt tgccacattt tgtaaaataa ttgttgcaat attatataga atatgataaa
     8101 actatattaa taaattaatt aaaaataatg agcctagggt agaggtagct gtattcaata
     8161 gtattcaata ggagctggta agcgttgata attgatgaaa gggtatatag ccgaagaaat
     8221 tttgttgacg gacaaatttt ctggacttat gcagaatatg cataaggctg tcacttttaa
     8281 agtggtgagc tacaaggtta cttggtctat gaaatttaca cgtccaaagt atacctttgg
     8341 gcgtttttat ttatcaaaat ttagcttgag ggggagaaag catgagtata attgttcaaa
     8401 aatatggtgg aagttcagta gcagatactg aaaaaataaa atcaatagca gaaaatataa
     8461 tagaacgaag aaaagaaaat ccacaaatgg taatagttgt atctgccatg ggaaaatcca
     8521 ctgatgaata tataacttta gcaaaggaat tgagtaatga acctagtaag agggaattag
     8581 atgctttaat gtctactggt gaaatgattt cagcatcttt attatcaata gcattaaatg
     8641 cacttggatg taaagctata agttataatg cttaccagtt aaatataaaa acgagtggtc
     8701 ttcatggaaa gtctcaaatt gatgatataa acgtaaacag aattaaaaat agtttagatg
     8761 aaggaaatgt cgtgatagta actggatttc agggaatcaa cgaagatggt gatgtaacca
     8821 ctcttggaag aggtggttca gatacatctg ctgtagctct tgcggttaaa ttaaatggaa
     8881 aatgtgagat atatacagat gttgatggaa tttattttac agacccaaga aaatattcaa
     8941 aggctagtaa acttgatgag attgaatatg aagaaatgct tgagttagcg agtttaggag
     9001 cacaagtaat gcattcaaga agtatagagc ttgcacaaaa atatggtgtt gaaatctatg
     9061 tcggacgtac atgtggtaca gaaaaaggaa catatataag agggggaaaa gacatgaaat
     9121 tagaggataa agtaataaca ggattagcta ctagtgatga tgacagttct ataacaataa
     9181 aagattttaa agctgaaaat atatctagct tatttgaaga tatagcaact ataggtataa
     9241 gtgtagatat gataagccaa actgcaccta ttttagataa gataagtgta tcatttacag
     9301 ttccaaaaga agagttggga gaatgtaaaa agatagtatc taaatataca gatgaagaac
     9361 atttagtgat tgataataat atcactaaat tttctttagt aggtcttggt atgaaaaata
     9421 cttctggagt tgctgctaaa gtatttaaga tattcaatga aaatggtata atgataaaac
     9481 ttataactac atcagagatt cgtataactt gtgctataaa ttctgatgat aagcaagtag
     9541 ctatagaaaa aatagcagaa gtatttaata tataaaattt ggggaggcta ttatgaaact
     9601 tcatggaact atgactgtaa ataatgatga attatatatt ggaggagtga gttgtttaga
     9661 acttagtaaa aaatatcaaa ctccacttta tgtatttgat gaagctttag ttagacaaaa
     9721 ttgtagagaa tatatagaat attttaaggc tagagaggga aaaaataagg ttgcttatgc
     9781 agggaaagct tttttacctc tttacatgtg taatcttata aatgaagaac aaatgtgttt
     9841 agatgtagtt tcaggaggcg agttatatac agcttataaa tcaaattttc ctatggaaaa
     9901 agttttcttt catggaaaca ataagactaa agaagaaata aacatgggat tagatttagg
     9961 tgtaggtaaa tttgtagtgg ataattttta tgaattagat ttgcttgaag cattatgtga
    10021 agataaaggt aaaactcaag agatatactt tagaataact cctggtatag aagctcatac
    10081 acatgattat ataaaaactg gacagataga ttctaaattt ggatttgctt tagtaaatgg
    10141 agatttattt agagcaatag aaaaattaag tgaatataaa aatattaaac ttgtaggtct
    10201 tcacgctcat ataggttcac aaatatttga tgtagaacca tacatagata cagtggatat
    10261 aatgatgaac ttggtaaaag aaataaaaga aaaattcaac atacaactaa cagaaattga
    10321 cctaggtgga ggagttggcg tttattatag cgaaggagat aaacctaaaa ctataaaaga
    10381 gttttgtgaa accataatca ctaaagcaga aacttcatgt aaagagcttg gaatagaagt
    10441 acctacattg gtaatagaac caggaaggtc aatagttgca aatgcaggaa gtacattgta
    10501 tacagtaggt tctataaaag atataaaaga tgttagggtt tatgtaagtg tagatggagg
    10561 tatgacagat aatgtaagac cttcgttgta ccaagctagt tatgagtgta gtattgtgaa
    10621 taaaattaat catgaggcta tgaatcatgt aacaatagct ggaaaatgtt gtgagagtgg
    10681 agatatatta attggagata taaacataat ggatattaaa agtggagata tacttataac
    10741 aacatcaact ggtgcatatg gatattcaat gtcttcaaat tataataaaa taccaagagc
    10801 agcagtggta tcagtatttg atggaaatga taagataatt tgtaagagac aaagctatga
    10861 ggacttatta gctttagaga tataaaatag agaagtatat aataaaaatt aaatattaaa
    10921 gtttattatg agcagatttg ctaggatatg tatctctaaa ttaatttttg gtttattgta
    10981 tcgttcgtag catatctgct tattttttgt tttactaaca aatttcttgt aataaatata
    11041 aaatatttta aaaactttat aattatgata aaatttaaag tatatgacaa ggagggattt
    11101 aatgagagga agagtaattt tattaatatc gatattatct atatttcttg taggatgttc
    11161 atttaataaa aaagatgaaa taaatttagt agagcaagga aaaacatatt tagaaaaaca
    11221 taactataaa aaagctatgg aatcattatc ctctgcactg gaagaagata gtactaatga
    11281 gaatgcaaga gctatgtata tgcaagctat gagaatgtca aatatgacgg agtttgaaga
    11341 attaaaaaat tatgagggtg caatcaagga acttaaactt atagagaaaa tcaaaaatgg
    11401 ttcctctgtt attaaagaag aggcaactaa aaagaaagaa gagttaacta aattagaaaa
    11461 agaacaaaac gaagcagagg aacaaagaaa gaaaaaggca aaagatacgg ctggagaaga
    11521 taggtataga gttgaatcag atgcaaggaa atcttcttat agtggaagta gtaaaaataa
    11581 taaatctagt tctaaaaaca atgataaaaa atcagatagt aataagtctg acaataataa
    11641 cagttcttct caaaatcaag aaaagccaaa taatagtaat ccaacaccag caccaactcc
    11701 acaagatact actggtggtt cagatagtgg tggaaattct caaaactcaa ataatcaata
    11761 agtataaaat atatgcataa atttaatgaa caaattatat attataaaaa atatctctta
    11821 gcaatttttt cttactaaga gatatttttt gagaaataat ttaataaata gcagttaaat
    11881 tttaaactaa aaattaaagt cacagtagag ataatctatt gtaaacgatt atctatatat
    11941 ttatcaacat aagtttttac aacagcagct attggtactg aaagaaaaac tcctatgaaa
    12001 ccaaatagag attgtcccac caaaatagca gatattaccc atatagctcc tataccaact
    12061 tgattaccca taacttttgg ttctataaaa tttccatcta attgttgcaa tataaataga
    12121 aatgcaatga cccatatagt tttttgtgga ctatataata tagtcatggc tattggaggg
    12181 attgcaccaa taaaaggacc aaaataggga attatattac ctaagaatat tattgaagct
    12241 aaaaataaag cattttctat tctgataaca aattgaaatc ctaaaaatgc aattatacca
    12301 ataataagag agtctaatat tctaccaagt aaataatcat agaaaatatc atgagtagtc
    12361 ctaaaaaatt ctataatgtt atctgccttg tttctagcag tagaagaata tagaaatctc
    12421 ttaaatccca atgcaatttt ttctttgtca tatataatgt atatagatag tataaatccc
    12481 attgtcatat taaatagcat agaagatata ctaagtgtat agattaagaa gtttttagga
    12541 a
//
LOCUS       DAYAOY010000116        12382 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYAOY010000116 DAYAOY010000000
VERSION     DAYAOY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.116"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            232..927
                     /gene="cas6"
                     /locus_tag="ACGI6B_003403"
     CDS             232..927
                     /gene="cas6"
                     /locus_tag="ACGI6B_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFG9752816.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            939..2678
                     /locus_tag="ACGI6B_003404"
     CDS             939..2678
                     /locus_tag="ACGI6B_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HFG9752817.1"
                     /translation="MLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKNFSNIDIL
                     EVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKKENLRPDK
                     NNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLNKIRLWIK
                     TNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIIDGKNYGLP
                     NDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSE
                     KGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHN
                     LKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGL
                     YTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMAD
                     KIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNA
                     QNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYY
                     NLIYTKSEENENE"
     gene            2671..3660
                     /locus_tag="ACGI6B_003405"
     CDS             2671..3660
                     /locus_tag="ACGI6B_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HFG9752818.1"
                     /translation="MNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALKFP
                     MKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQKVL
                     ENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPKAK
                     DKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKNDY
                     KKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDLIC
                     INIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3662..4402
                     /gene="cas5b"
                     /locus_tag="ACGI6B_003406"
     CDS             3662..4402
                     /gene="cas5b"
                     /locus_tag="ACGI6B_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HFG9752819.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4426..6984
                     /locus_tag="ACGI6B_003407"
     CDS             4426..6984
                     /locus_tag="ACGI6B_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFG9752820.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   7109..10044
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7174..7203
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            10129..10623
                     /locus_tag="ACGI6B_003408"
     CDS             10129..10623
                     /locus_tag="ACGI6B_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFG9752821.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            10649..11656
                     /gene="cas1b"
                     /locus_tag="ACGI6B_003409"
     CDS             10649..11656
                     /gene="cas1b"
                     /locus_tag="ACGI6B_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HFG9752822.1"
                     /translation="MLILGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFC
                     LNEVSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRRE
                     EIAKSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEG
                     DIWQTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQ
                     SVSFLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLND
                     EGKQIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDG
                     L"
     gene            11867..12157
                     /gene="cas2"
                     /locus_tag="ACGI6B_003410"
     CDS             11867..12157
                     /gene="cas2"
                     /locus_tag="ACGI6B_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFG9752823.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT         5328 a         1082 c         1881 g         4091 t
ORIGIN      
        1 gaaatttatt atcaataatc taaatataga aattaaaaat gataaagaga ttgtaattat
       61 atttttttga aactttgtga ttttatttgt cgaataaaaa ataaaaaatt ttaaaggaaa
      121 aagttaaaaa ataaagaatt ttataactat aaattatatt ttagagttta ttgttattaa
      181 atagatgaaa aaatcctaat atggattatc agaaaatgag gaggttgtta attgataaaa
      241 tgtcttgaaa tgtctgtaaa agtgtatctt cttgaaaata tttctttaga taatacatca
      301 aaagaaatac gtaaattaat agatgataca ttaattatag aagaaaaata taaggaattt
      361 cataataaag ctggatttaa gaattattct tttaatttat tatacccatt agaaaaagat
      421 aaggtttata agaaggattg tatatatact tttattatta gaactgtaga tagtgaatta
      481 ggaatgtatt ttatgaataa tttaaaaaat cagcattctg ataaaataaa ggttattaca
      541 atgagtaaaa aagtgatccc taaaaaacat atagaaagta tttactctgt cactccaata
      601 atagctaaat ttgaggatgg gtattggaga agtaataaaa cagtagaaga tttagaaaaa
      661 agaatcagag aaaatttaat aaaaaaatat aatgaattta ctaacagtaa aatagatgaa
      721 gattttgaat tatttcaagt tattaaattt ataaatagga aacctatttc tactaaatat
      781 aagaatatat ctttacttgg tgataaaata agttttaaag tatcagaaaa tgatatggct
      841 caggaaattg caaattttgc acttggagtt ggagtcggag aaattaattc tcgtggattt
      901 ggttttgtaa actatagatg gttataggag gtgaaaactt gttaaaggat tgtttagaga
      961 tttttgaaaa agaatacaaa tctttaggaa atagattgat aactgatagc tatgtcctat
     1021 cctatggaac atatatattg gtgaaaaatt tttcgaatat agatatatta gaagtttcta
     1081 aaaataatgt ggatagtaca gaagatatat accataaatt ttgtgaactg gattatttat
     1141 caaaactggt gtctatagat aaatcagttg attccaaaaa aataatacat agtaataatt
     1201 atctttcatt ttttgtaaaa aaggaaaact taagacctga taaaaataat aatgtaaaac
     1261 ttacagaaga aattattgat aattattatg atgctttgtt aaacccaaga attaaatata
     1321 aatctgataa aagaaaaaaa gaaatctatg attcaataga aaataaatat ggtaaagtgg
     1381 atgaaaataa attaaataaa ataagactat ggataaaaac aaatatattt gatttagttg
     1441 aatctgttaa aaaagataaa aattatttaa aaatattttt tgatgaaaaa atagagttat
     1501 acaatgatga aaaccaaaaa tatttatatc caaatatata taataaaaat gatttcaatg
     1561 taattattga tggtaaaaac tatgggttac caaacgataa tatgcaatta aatgaaaaaa
     1621 agccattttt gaaattaaaa acaagaatta gaaattcaac tccttatttg attagtgatg
     1681 atgaagtttt gttacagaaa gctttttttg attatctaac gaataaagca gcgaatggag
     1741 aagaagtaat atatataagt agcgaaaagg gaattcatag aaaaactgat ataccaaatg
     1801 aatttagtgg ttatatactg agaataaaaa agggtaaaga attagaaata catgattttg
     1861 atactgtggt aaatttaaac actaaaataa aaagaataaa tgtagaatgt ataataccaa
     1921 tagaatatga aagacataat ttaaaaccaa atttagagat aggatatatt aataatctta
     1981 taaaattgga aaaaacgatt gatactgtat tctttaataa atttatgtgc aatagttatt
     2041 ttaaagaaat taaaaatata aacttaagtg atttagttct aagggatata cttattagac
     2101 atagagaagg tttatatacc tggttttata aagggagaga tagtatagta aagcaaactt
     2161 tttcaaacta ttctatgggt ataattgaaa attctataaa aaacaataac tacataagag
     2221 caaaggagca atataattta agaatagcta taataaatta ttttcatgga ggaagtaata
     2281 tggcagataa aataaaagtt attagtgaat ctttatttga aaaaataaat tctataaaaa
     2341 cgaaatgtat acaaagtgat gaagaatatt attttgcagt tgggcaatta gcatcgtatt
     2401 ttatatctaa aaataagtca aaaaataaaa atcattctct gattaatcct atactaaatg
     2461 cacaaaatag aaaaaaaatc atgttagaga tagaaaaatt atttaaaaaa tataattatg
     2521 atataagtta tgacaacaat aggcgattta agaatctata ttctatgatt gtaggatatg
     2581 aggtagaaga aaaaaaagtt aatagagatt tattaattgg tggatatcta tattacaatt
     2641 taatatacac aaaaagtgag gagaatgaaa atgaatagaa gagtatatgg agtattaggt
     2701 ataaaatcta taatgtcaaa ctggaatgct gatttttctg gatatccaaa gacgacatca
     2761 acaggagaag tttttgggag tgataaagca cttaagtttc ccatgaaaaa aatgtgggaa
     2821 aatgaaggtg aaaaagtttt atatgttaaa tcattaaaaa tagatgaatc taagaaagga
     2881 gaaataaaaa ttagaccaaa atcattaaaa gagagatatg aagaattgtt tgatatagag
     2941 gacctaaaac tagaaaagaa tcaacaaaaa gtactggaaa atctatttaa agctattgat
     3001 gttaaaaatt ttggtgcaac atttgcagaa gaaggaaata atataggaat tatgggcgca
     3061 gtacaaattg gacaaggatt taataagtat gaagatatta atgttgaaga acaacaaata
     3121 ttatccccat ttagggatcc aaaagcaaaa gataagaaaa agtcaacaaa tgaagaagaa
     3181 aaagaagatg caaattcaac tacattagga aaaaaaatag taagtgatga ggctcattac
     3241 ttttatccat tttcaataaa tccaaaagct tatgaagttt ttgttaatat gggtgtgact
     3301 gatggatata ctaaaaatga ttataaaaaa tttaaagaag ctgctttagt aagtgcaact
     3361 gcattcaata caaattcaaa gcttggttgc gaaaatgaat ttgcattatt tgttgaaact
     3421 gatgaagagt tatacctacc agatttggca caatatataa cttttgaaaa gagagaaaaa
     3481 tcaaaagatt taatttgtat aaatataatt catcttatag cagaattaaa agatgatata
     3541 aagagcgttg agatatatta taacccatat aaaatagaaa taaagccttt taaagatgaa
     3601 atattaccta agatagataa agttaaattt atgaacatat ttacaagaaa agaggtataa
     3661 tatggaggtt ttgaaattta acttaagtgg aaaaactgct ttttttaaaa aaccagatgt
     3721 aaatagcata atttatttta cgtatggaaa catacataaa attgcactta tgggtatttt
     3781 aggagcttgt ttaggacttg gtggatataa tcaacagagt aaggaagatg agtatccaga
     3841 attctactct ttacttaagg atataaaaat gtcaatagtt ccaaagcatg aatttggata
     3901 tataaataaa aaagtccata tatttaataa ttctgtagga tatgcatcaa aagaagaagg
     3961 tggaaattta ataataaaag aacagtggct tgaaaatgtt agttgggata tatatataca
     4021 attagaagat tcaactacaa gtagagagct taaaagaaga tttttatcta ataaatttgt
     4081 atatatacct tacttaggca aaaacgatca tatagctaat ataagtgaaa tagagataat
     4141 tactgatttt tacaaaacag aagatattaa cagagtggac agtttcttta taagagagga
     4201 ttttgagttt gaaaaatctt attttgatga taatgatgat tataaagagc catttaaata
     4261 tcaggaaaaa cttccttata gactgcaccg aatgactaat caatatattt tggaaactat
     4321 catatttact aatatgtcag ttaaattgat taataaaaaa gaggtatatc aagtaaaggg
     4381 tttaaattta atctttattt aaatttatag gtttaggaga attatatgta ctttaaaaaa
     4441 gttgagttgt ttgatataag taataatata aataattgca aaaatattta tgctcatata
     4501 aataaagaca ggaagccaga aactttaaaa gaacattcag acttatcata ctcatatctt
     4561 atgaaattat gtgtaaaaaa agatttaagc tcttgcctag agagaataga gaataaattt
     4621 tttaaaaaag attctttttt aagtaagaat ttttatagag agattatctt gaatatggtt
     4681 tatatgcatg atataggtaa gattaattct tcatttcagt ttgtaaaaat gcaaaataaa
     4741 aattttaaaa aaatttcact tagatatact aaacattctt ttatctcttc aatgctatat
     4801 tttgattact attttgaaaa gcttaaaaaa tatgattttg aagaaaaaaa aatattgagt
     4861 ttatttttga tgataaatac atatacaata tcaaaacatc atggggttct gaagtcattt
     4921 aaagaatttt tatttttatt taaagatgag tttataaaca ttaaagaaaa ttttaaagat
     4981 ttatttaaaa acaattataa tttagagctt aaatcagttc ccaaaactat agaaaaaatg
     5041 gtaagaggtg tagaagcgta tttagatgat agccatataa aggataactt agctatagac
     5101 ttatatatat attcaaaatt gatgttttca attgttacat cttgtgattt ttatgctaca
     5161 tcagaatata atagtggtaa ttctatagat gattttggga caattgataa ttataataag
     5221 tattatgatg tattaaaaaa atctgaaata tataagggga tgaaaaaata tagggaatac
     5281 ttaaataaca atagtgaatg cccatataaa gagaatgata taaacagatt gagaacagaa
     5341 atgagtatag aagctgaaga tgcaattaca cataatacga gaaaaagaat attttattta
     5401 gaagcaccaa ctggaagtgg aaaaactgtt acatctataa atctagcact gaaatctata
     5461 gagttaaatc aatctttaaa aaagatattt tatatatttc catttaacac attagtagag
     5521 caaacaaaag atgtatttat aaatgaaata tttaattctg ttaaagacat acaaaaagat
     5581 gttgtagtta taaattcaat aacaccaata caaacagaag ataaagaaga agataataaa
     5641 aatgttgaat attgtgttac taaacaagaa attgattatg ataaatcatt gttaaataga
     5701 caatttttac actatcctat agtacttaca actaatatag gtttttttaa ctatttgttt
     5761 ggtgtatcaa gagaagaatg ttttccattg atacatatga taaatagtgt aattatatta
     5821 gatgaaatac aaaattataa aaatgagata tggaaagaaa ttattttgtt tttagaaaaa
     5881 tatgcagata tattaaatat aaaatttatt ataatgtctg caactttacc tagattaaat
     5941 aaattaggat gtaatgatga taattttgca tatctagtaa aaaacagaga aaaatttttt
     6001 aaaaatcacc tttttaaaga tagagtaaac ataagttttg agatgttaaa ttcagaaatt
     6061 aatgatattg aagaaatatc agaaaaggtt atagaagaag cagaaatata taataagata
     6121 ctgatagagt ttataaagaa atcatcagca ctaaaatttt ataaaaatat taaagaaaaa
     6181 ttgaagtata agttagaaga tgttttttta ttaacaggtg atgataataa attagaaaga
     6241 aaaaaaataa taaataaaat taaagatatg aactctgtaa ttttggttgc tacacaagtt
     6301 gtagaggctg gagtagatat agatatggat ttgggattta aagatatatc tacaattgat
     6361 tctgatgaac aatttttggg aagaataaat agatcttgta aaaaattaaa ttcaaaagta
     6421 tactttttta atttagatga tgcaagtaag atatataaga aagatgttag aaaagagaga
     6481 catttaactc ttaatgagga aagtaataga aaaattattt tagataaaga ctttgaaaag
     6541 tattatgata aaataatagc cagaatagaa gaaaataagc ataaacataa tgacaagaat
     6601 atagaggttt ttataaaaga tattgtggga aatttgaatt ttacagaggt aaaaaaatgg
     6661 atggctttaa taccagattt aaaagagtat acaatctttt taaatactat agttaaagat
     6721 gaatctggaa atatgattgt aggaagtgat gtatggtatg aatatatatc actactaaaa
     6781 aatgataatg ttgagtattc tgaaaagagg gtgaaaatgt ctgaaattat ggaaaaacta
     6841 gattatttta cgtataaagt tcagaaattt gataattcat ttaatgattt agttggagat
     6901 attttttata ttgatgatgg aagtaagtat tttacagagg gaaagtttga tagaagtaaa
     6961 tttaatcaaa atgaatttct ataatgtatt tagattatat agagaatatt tatcccaagt
     7021 taattaatta gaattagttt aaaactattg aaatatagat attttatagc ttatatattt
     7081 tttctaggat tagctgggat aaaaaatctg tatattagtg ttttcagtgt atttaagggg
     7141 attatatttt taagaagtac tgattttaaa gtggttaaac agtaacatga gatgtattta
     7201 aatttttact acataattaa atcgtagtca ataccgtaca ggttaaacag taacatgaga
     7261 tgtatttaaa tctctgaata gccaatcaac tttgctgcat tactcttgtt aaacagtaac
     7321 atgagatgta tttaaatgat aatagttata tatatttttt acaatatatt attgttaaac
     7381 agtaacatga gatgtattta aatttacttc tcctgttttg aaatctatat attcatcatg
     7441 ttaaacagta acatgagatg tatttaaata taatattgat acaaatattt tgggtaaatg
     7501 gacttgttaa acagtaacat gagatgtatt taaattcgat atggtaacaa atttataaaa
     7561 gatatagaat gggttaaaca gtaacatgag atgtatttaa attaataatg cacctatttt
     7621 aacagtacct ggtctaaagt taaacagtaa catgagatgt atttaaattc aagtctagga
     7681 gacaacataa atagtacaga tgggttaaac agtaacatga gatgtattta aattagtaaa
     7741 accagatgga actgttgtaa taccaccaaa gttaaacagt aacatgagat gtatttaaat
     7801 ttaaaaacat cttgaggaat atctttaaca gtaattgtta aacagtaaca tgagatgtat
     7861 ttaaatgcat tttcaggtgc atgataaata ggtaaattag atgttaaaca gtaacatgag
     7921 atgtatttaa attttttgta attagccaga ttatcaactc ccaaaatagt taaacagtaa
     7981 catgagatgt atttaaatta aatatcgtcc ttcgtagtta aagcaacacc agaaggttaa
     8041 acagtaacat gagatgtatt taaatcgtaa acaaaattta tcaagagaag aatttctaag
     8101 gttaaacagt aacatgagat gtatttaaat ttatatttga taagagtatc aaatcgactt
     8161 aaaatagtta aacagtaaca tgagatgtat ttaaattcaa tcttaataca cagacatttt
     8221 taaaaacaaa acgttaaaca gtaacatgag atgtatttaa ataaatagac aaatattgaa
     8281 tagctctgtc ttcatcaagt taaacagtaa catgagatgt atttaaatat atttacaaaa
     8341 tctctaacat attttttaac ttctgttaaa cagtaacatg agatgtattt aaattctttt
     8401 aaagagtcct cgggagcctc gaaaacttca gttaaacagt aacatgagat gtatttaaat
     8461 tactcaaata aatttctaat ttgtttatct actaacgtta aacagtaaca tgagatgtat
     8521 ttaaattaga atttacaaca atataagcct ctttaaccat aagttaaaca gtaacatgag
     8581 atgtatttaa atatgttttt agtagtacat ttaataaaac tttttaaagt taaacagtaa
     8641 catgagatgt atttaaatga aggaaccgta ccttgataaa tcacgaccgt acccgttaaa
     8701 cagtaacatg agatgtattt aaattgtata tttgatgttg ttatttctcc tgtgtataat
     8761 gttaaacagt aacatgagat gtatttaaat gaagttccac ccccaccacc cccactactt
     8821 tgagaagtta aacagtaaca tgagatgtat ttaaattaac aatagagtaa atataataaa
     8881 caagcataca aagttaaaca gtaacatgag atgtatttaa atcccctttt gcatggtact
     8941 agacacgcat atatggtggt taaacagtaa catgagatgt atttaaatac gcctatatgc
     9001 ggagtaacag atttttgcct ggatggttaa acagtaacat gagatgtatt taaatactgt
     9061 tttgggatag actggaatta aatggtctgt gttaaacagt aacatgagat gtatttaaat
     9121 ttttggaatg aagcatcaac agataataaa gaaaaagtta aacagtaaca tgagatgtat
     9181 ttaaatcaag gaaacaaaag agaagtgaaa agaaatataa tgttaaacag taacatgaga
     9241 tgtatttaaa taatctttta aatggaaatt aagtaataaa agagagggtt aaacagtaac
     9301 atgagatgta tttaaattca attaaataag cattaatctt agtaaaatca aacgttaaac
     9361 agtaacatga gatgtattta aattttcttg tgttaattga acccccattt taagctccag
     9421 ttaaacagta acatgagatg tatttaaatc agaatacagc ttctatatct ggatacattt
     9481 gtcttggtta aacagtaaca tgagatgtat ttaaatgtga aaagtaatac aggtatagag
     9541 tctaaacgct cggttaaaca gtaacatgag atgtatttaa atcaatgtat cctctttttt
     9601 agttttggag cctcttttgt taaacagtaa catgagatgt atttaaatta gttagcacat
     9661 tttatttcaa gccttaaaac attcgttaaa cagtaacatg agatgtattt aaattaattt
     9721 gcacacttaa cctctaatct taaagtatta gttaaacagt aacatgagat gtatttaaat
     9781 aacacaatat tatctttaat agaattaaac aaatgagtta aacagtaaca tgagatgtat
     9841 ttaaattttt gttggtcttg tgcttctttt aaaagctgat tagttaaaca gtaacatgag
     9901 atgtatttaa atttaaaaag ttctgttatt aaaacatcca aaccaatcgt taaacagtaa
     9961 catgagatgt atttaaattc tgtaccatct agccaaaccg tttttgaagt taaagttaaa
    10021 cagtaacata agatgtatct aaattactct atcataaaat tttaatatat taaacaaact
    10081 aatatgtgat atacttatgt ataaataaca aaataaatag gtgatgttat gaaaattaca
    10141 ggaactttga taaattacta tttccattgt aaaagacaat gctggttgct tggtaataga
    10201 ataaatttgg aagaaaatag tgaagatgta aaaataggta gacttcttca tgaactaaaa
    10261 gaagaaaaaa caaaatataa agaaattgca attgaaaata taaaaataga caaattaaca
    10321 agagaatatt tagtagaggt taaaaagtct gatgctgata tagaagctgt taagtggcaa
    10381 gttcttttat atttaaaaaa gttaaaagaa aagggaattg taagaaaagg gaaaattgaa
    10441 tttatagaaa aaaataaaac taataaaaaa atagtctttg tagatttgag tgaagtatct
    10501 gaagaacagc ttatatctat agaaagagaa attgttaatt taataaatag tgataaagca
    10561 ccatgtcctg atttaaaaaa aggatgtaaa aaatgtgcct attatgaata ttgttatata
    10621 taaagaggta taagaaacat ataaggaggt gttaatattg ggaaagacaa aatatattac
    10681 ctcaatgggt gaactcacaa gaaaagataa ttctatatgt tttagaaaaa atggaaggaa
    10741 tgtatatata ccagtagaaa atactagtga aatattctgt ctaaatgagg tatctttaaa
    10801 ttcaaaatta ctggattttt tatcaagagc aaatattgtt gttcattttt ttaattacta
    10861 tggtggatat agtggaactt tttatcctaa agaatattta ataagtggaa aagtaacagt
    10921 ggcacaggta aaaacctttg aagatagaag agaagaaata gcaaaatcca tagtcaatgg
    10981 tataaggatt aatgcaataa atacattatc gcattactat aagcatggaa attcagaaat
    11041 acaaccatta ttatcacatc taaaaaaaga ttctataaac aagttagaaa aatcaaataa
    11101 tataaaagaa atattatcag tcgaaggaga tatttggcaa acattttata cttcctttaa
    11161 gtatatttta agagaggaat ttatttttaa taaaagagtt aagagaccac cagataatcc
    11221 cttaaacgca atgatatcat ttggtaacag tattttatat gctaaaactg taactgctat
    11281 atataataca catctaaatc aaagtgtaag ttttttacat gaaccaagtg aaagtagatt
    11341 ttctttaagt ctagatttaa gtgaaaattt taaacatgtt ataactttta aaactatatt
    11401 tgatttagtt aataatagaa aaataaatat tgaaaaacat tttgataaaa atttgaacta
    11461 ctgtgttcta aatgatgaag gaaagcaaat ttttataaat tctttagaag aaagattaga
    11521 atcagtcaaa gaacatccaa aattaaaacg aaaagtaagt ttaaaaacct gtataaaatt
    11581 agatgcttac aaaatgttaa agtgtataat ggaagaaaaa agttttattc catataacat
    11641 aaaagacgga ttataattta aacataaagt aagtatttta atatagaact ttgatatata
    11701 taagaatgat aattatgcta ttataagtat acataatata ttattgaaat aatataagtt
    11761 tttagaagta ttttaagatg agaaaggaga aaatactgaa aaataattat tctattataa
    11821 tttgccaagg ttttgtatat ttttcagttt aaatattata ttacatatga aaaagaaaaa
    11881 caatttaaat tataattatg tatttgtagt ctatgatgta ggagaaaaaa gagtaagtaa
    11941 agtttttaaa gtgtgtaaaa aatatctctc gcattttcaa aaatcagttt ttagaggtga
    12001 aataactcca tctaagatta tgagcttaaa acgtgattta gaaaatatta tagataaaga
    12061 taatgatttt gtatgtataa taaagttgat gaattctaat acatttgaag aagaaatatt
    12121 agggaataat agtaattgtg aaaatatgat attataaatc taattactat atttgtaaaa
    12181 aataatagct tattaaagag tagtaaaatt gggatataat ttatgataga gactgcaagt
    12241 agatttttgt gaaaaggatt tacttgcagt atttttgtta tgtataactg ttaacattag
    12301 tgtacaaaac attatcaata gcatttttct ataatgaaga aatactatta taatgggaaa
    12361 attttattaa tactaggtaa ta
//
LOCUS       DAYAOY010000117        11842 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAYAOY010000117 DAYAOY010000000
VERSION     DAYAOY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.117"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            40..465
                     /locus_tag="ACGI6B_003411"
     CDS             40..465
                     /locus_tag="ACGI6B_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9752824.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            458..895
                     /locus_tag="ACGI6B_003412"
     CDS             458..895
                     /locus_tag="ACGI6B_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9752825.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            879..1064
                     /locus_tag="ACGI6B_003413"
     CDS             879..1064
                     /locus_tag="ACGI6B_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752826.1"
                     /translation="MIILNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELY
                     SFSEVEEIINKFMKGVS"
     gene            1065..2375
                     /locus_tag="ACGI6B_003414"
     CDS             1065..2375
                     /locus_tag="ACGI6B_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9752827.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVGVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            2397..2867
                     /locus_tag="ACGI6B_003415"
     CDS             2397..2867
                     /locus_tag="ACGI6B_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9752828.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            2920..3177
                     /locus_tag="ACGI6B_003416"
     CDS             2920..3177
                     /locus_tag="ACGI6B_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752829.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            3182..3622
                     /locus_tag="ACGI6B_003417"
     CDS             3182..3622
                     /locus_tag="ACGI6B_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9752830.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            3884..4675
                     /locus_tag="ACGI6B_003418"
     CDS             3884..4675
                     /locus_tag="ACGI6B_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9752831.1"
                     /translation="MGLFSKKNKKPCCICGSEKGLMPSIEGENFCTACNCKYIDFSEN
                     ILKVTSIMKMMSNSEGMKKFIEVEKKNLKLLEKFTETKSINSSISFDEEQNLLKISYK
                     NRNQILVEKIIKFDDILEFELLEDGETIVKGGLGRAITGGVLFGGTGAVVGGITGKKT
                     SRKVVEIFKIKITVKDINNPIEYINLINSKVKTNSSIYQKAFSDAQEILSILSIITKY
                     NVMEDKKKSISSSTADEILKYKNLLDMEAITQEEFDAKKKELLNL"
     gene            4742..7084
                     /locus_tag="ACGI6B_003419"
     CDS             4742..7084
                     /locus_tag="ACGI6B_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFG9752832.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMNNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIRESDNQQRRLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLSMINDGQLSDGGLNKMIFQSAERSRASYLDTAK
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTLGQIFTSVKNNAVMVFGAIQKKIEDTVSSRGFRTFITDVKDSLYVLGAVGFNV
                     FSGFINLLSSPAFQNFFNVMIVGTSLVVQGLGWIITQALSVANVFSRNWSIISPIILG
                     VVSAISTYIIALGIMQAVTIAGTVANWMSWTSEVIRQRGILGTARALAIGKAEQWGFN
                     AAIYACPIFWIVLGIIAVISAVFILVAVYNHFAGTSISAISLIVGAWYWLCAVVYNVI
                     VGIVNAINICVVEIANIFRTGLYAVQCFFIDMANAGLKAGVNLDKAFDKFATNLANGI
                     IKAVNIAVKGLNWLVQQINKIPGIDLPQMKEFQKVNTVIGTKTTFKPIQKPPEPKAWK
                     PELVEYKNLKSEFIKGYDVGHQLQNKFKDTFDISKIAEKAKKDLGLDDLWDKKYGLGD
                     GLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVN
                     IKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7100..7801
                     /locus_tag="ACGI6B_003420"
     CDS             7100..7801
                     /locus_tag="ACGI6B_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9752833.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDADNGSDVKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPTPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     VPKYTIYKGQVLKLG"
     gene            7794..9767
                     /locus_tag="ACGI6B_003421"
     CDS             7794..9767
                     /locus_tag="ACGI6B_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9752834.1"
                     /translation="MADELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDEFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHMG
                     RKTLESIRDKYAPLYDSPLNKHWVPGVNKFYKQITGNAYSSNSAGTGVRSNEEAEKNL
                     KDITYQVQNSNNANTSTNNNSKVNKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSNDSRGKLLCNINDVKAGDLVFFAYNQGKGNVHHVGLCIGNDK
                     FIHSPNTGDVVKISSLSGRQKQKHDFARARRFF"
     gene            9781..10041
                     /locus_tag="ACGI6B_003422"
     CDS             9781..10041
                     /locus_tag="ACGI6B_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9752835.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            10046..10465
                     /locus_tag="ACGI6B_003423"
     CDS             10046..10465
                     /locus_tag="ACGI6B_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9752836.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            10466..11515
                     /locus_tag="ACGI6B_003424"
     CDS             10466..11515
                     /locus_tag="ACGI6B_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9752837.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINSIASNL
                     NVDKDSIVLRGEVVG"
     gene            11508..>11842
                     /locus_tag="ACGI6B_003425"
     CDS             11508..>11842
                     /locus_tag="ACGI6B_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9752838.1"
                     /translation="MDKEINLINYLPQILRDKEEYIKVFNVENKEIKTLHEKLNDLSN
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELT"
BASE COUNT         4706 a         1315 c         2364 g         3457 t
ORIGIN      
        1 tcaagaaata atacttgagt tatttaagga taaagcataa tggctagatg gggcagtgtt
       61 gattttagag agtttaaaag agtttgtaaa aagatggaga agcttacaaa gattgattta
      121 gataagtttt gcaaagatgc agcaagagaa ttagcagcaa gactcttagg aaaagtaatt
      181 agaaggacac cagttgatac aggattctta agacaaggat ggaatggagt ggcttatgct
      241 aggtcgcttc ctgtgtataa acaaggaaat aattatatta tagaggttgt taatccgact
      301 gaatatgcaa gttatgtaaa tttcgggcat agaactaaag atggaaaagg ttgggttaaa
      361 ggacaacatt tcttaacaat ttcagagatg gaactacaaa gccaagttga taagattata
      421 gagaaaaagt tattaatatt acttaaagga gtatttgatg cttaataata ttatagatgg
      481 aatatcagta aagttagata aatcatttgg agaaaaatat acaatttata gtgaggatgt
      541 agagcaaggt attaatgaac cttgtttttt tattgttcct ttaaatccaa gcaagacacc
      601 atatccaagc gggagagaat taaagaaaaa ttcttttgat gtacattatt tccctcgttc
      661 agaagctaag aattttgaaa taaatgagat agctgagatg ctactggagg aattagagta
      721 tatagaaatt gatggagatt tagtcagagg tacaaatatg aattttgaaa ttatagacaa
      781 tgttcttcac ttctttgttg attataacta ttttactata aaaaataatg ataccaataa
      841 gatggataca gtagagttat tcggtggttt gaagagaggt gataattttg aataaaacat
      901 taagcaaaga agatgactac aagtttacta aggagcaaat agttaattct aagaagtatg
      961 taaatagaaa agacttatta aatgcaattt taaaagaaaa tgagttatat tccttctcag
     1021 aggtagagga aataataaat aaatttatga aaggagtgag ctagatggcg ttaggtggag
     1081 gaacatttgt aacacagaat aaaatattac caggtagcta tataaatttt atctcagcta
     1141 agagggcaac cagttcatta tcggatagag gtattgttgc aataccttta gagttagatt
     1201 ggggcataga tgaagacgta tttcaagtaa ccagtgatga ttttgagaag tattcagtga
     1261 agtatttcgg atatgattat actcatgaga agctgaaagg tttgagagat ttattcaaaa
     1321 atataaggtt gggatatttt tataaattaa ataaaggcgt taaagccagt tgtactatag
     1381 ccacagcaaa atatagtggt atcagaggaa atgacttaaa agtaacagtt acaacaaata
     1441 tagatgataa tgctaagttt gatgttgtaa cacttttaga taataagaag gtagatactc
     1501 aaatagctaa agtgattaca gacttacaag ataatgacta tatcacttgg aagaaggatg
     1561 caacactaga agcaagtgca gggctggtat ttactggtgg aactaatggc gaagctgtga
     1621 caggagcaga gtaccaagct ttcttggata aaatagaaag ctatagcttt aatgctttag
     1681 gatgtttggc tacaacaaca gagattaaaa gtttatttgt agaatttaca aagagaatga
     1741 gagataaggt aggagcaaag tttcaaacag tactatataa gaaaagtgat gcagattatg
     1801 aaggtgtagt gtctgtagaa aataagatta aagataaaga tttagttgaa tctagcttga
     1861 tttattgggc tactggagct atagcaggat gcgatataaa taaatctaat actaataaaa
     1921 agtatgatgg tgagtttgat gttggtgtaa attacactca aatacaactt gaagaagcac
     1981 taaagagtgg taaatttata tttcataaag ttggtgatga agttcatgtg ttagaggaca
     2041 taaatacttt tgtatcattt acagatgata aaaatgacga tttttcaagt aaccaaagtg
     2101 ttagagtact tgaccaaatt gctaatgata tagcgacttt atttaataca aagtacttgg
     2161 gtgaagtacc aaatgataaa tctggtcgta tctcgttttg gaatgatgta gttaagcatc
     2221 atgaacaact gcaaaatatg agagcaatag aagatttcaa agctgatgat gtttctgtag
     2281 aacctggaag cgacaagaag actgttgtag taagtgatgc tgtaaaagtt attagtgcta
     2341 tgagtaagct ttatatgact gtttcagtta gttaacaata agaaaggaga ataataatgg
     2401 cacaacaaat aaaagcaaga gatacaataa gtgcatctaa ggcagagtgt tttgtaacta
     2461 taaaaggtaa aagatataat tttatgcaag ctattaactt agaagctaaa atggaaaaga
     2521 ataagagtga gatacctata ttaggtagta ctacaaaagg aaataaatca acaggaagta
     2581 aatattcagg aaatgcaaca ttttattata atacctctat atttagagaa ttgttgtatg
     2641 agtataaaga aactggtgag gatatttact tcgatataca aattaccaat gaagacccaa
     2701 caagttcagt gggtcgtcaa actataatac tggaagattg caatatggac tcaggcataa
     2761 ttgctaaatt tgatgctgat ggggagtatt tagatgaaga tatggatttt acttttgaga
     2821 attggaaatt agttgagaaa tttaatatag caaatggtat ggagtaaaat acacatttat
     2881 gatttatgta tgtgtatttt ttatttataa gaataggaga tgattaaaat taaggataaa
     2941 tatgagataa aagattcaat ttcttttgat tatagcaata aaagaccttt ggaagaacgt
     3001 gtcagcgaga tgtataaaaa ggcaggaaaa tatcttatag atatttcaga taagttagca
     3061 acagatacaa ttgatggttc gtcattaaag ccaataatca taaaatttga aataaatgaa
     3121 gctggtgttg caacaataga aaaacaaaca aaatatttgg tgatggaggt agaataagaa
     3181 tatgggagat ttaaacgctt ttttaagtca aaatgcaata aaagtagaga atagaaagta
     3241 tgtggcaagt gaaaggttta taggagaaga tggaaaagca atcgaatggg aacttaaagc
     3301 aatagattca gatagggaca gacaattaag aaaagattca actataagag tacctatatt
     3361 aaataaaaaa gggaaagcta caggacaata cactagtgaa acagatttta atacttacac
     3421 tttgaaatta tgtgtggaaa ctatagtatt tccggattta catgatgcag aattacaaaa
     3481 tagttatggt gtaatgggtg cagaggagtt attaacaaca atgttgactc ctggcgaata
     3541 tacagacctt tcaagtgagg taggagaggt aaatggtttt gatagaactt ttgaagataa
     3601 agtagaagaa gcaaaaaact aatttatgaa ggtgattatg attctaatat agctcattat
     3661 tgccttcata agtttaaatg gaaaccgcat gaatatatgg atttaccagt tgatgaaaaa
     3721 gcatttgttg ctgcttctat agatatcaaa gtagaagaag aaaaagaaga agctaaaaaa
     3781 gctgacaaag atgcaaagag aggtagaaga agataatctc tggtgtaaaa attttactaa
     3841 tatagtataa tatatttaaa aattgtactg ggggattaaa taaatgggat tgtttagtaa
     3901 aaagaataag aaaccttgtt gtatttgcgg aagcgaaaaa ggacttatgc catctataga
     3961 aggtgaaaat ttttgtacag cttgtaattg caagtacata gatttctctg aaaatatttt
     4021 aaaagtaact agtattatga aaatgatgtc aaactcagaa ggaatgaaaa agtttataga
     4081 agttgaaaag aaaaatttaa aattgttaga gaagttcacc gagacaaaaa gtataaattc
     4141 ttcaatttct tttgatgaag agcaaaatct tttaaaaata tcatataaaa atagaaatca
     4201 aatattagta gagaaaataa taaaatttga tgatatatta gaatttgaac ttttggaaga
     4261 tggagaaact atagttaaag gtggtttagg tcgtgctata actggaggag tcttattcgg
     4321 aggtacaggg gctgtagttg gaggaattac aggtaaaaaa acaagtagaa aagttgtaga
     4381 aatatttaaa ataaaaataa ctgtaaagga cataaataat ccaattgaat atataaattt
     4441 aattaatagt aaagtaaaaa caaactcttc tatttatcag aaagcttttt ctgatgctca
     4501 agaaatatta tcaatactat caataattac aaaatataat gtaatggaag acaaaaaaaa
     4561 gtctatatct agttctacag ctgatgaaat attaaaatat aagaatttgt tagatatgga
     4621 agctattaca caagaagaat ttgatgctaa gaaaaaagaa ttgctaaatt tataatatat
     4681 aagcacttac ttaaaaataa gtaagtgctt atattatgtt aaaaaagaaa ggaggttaaa
     4741 aatggcaact atacaaacat ctatccgaat tttcgacgga atgacacctg cttttagaca
     4801 catgaataat gctatgaata ttgtattaag ttcattcgag caattacaaa gaacatctag
     4861 caatgctata gatgctaata gtattagaac agctagagaa gaactagcac gtgcagaagc
     4921 tggctttgat agactagaac aacaaataag agaatcagat aatcaacagc gaaggcttaa
     4981 tgaggatata aataagggtg caagttctac agatagatta gttggaagtg caaagaagct
     5041 agcagcaacc tacttaggta taagaacgtt aggaggtcta ggaaatttaa gcgaccagat
     5101 gacaagtact aacgcgagac ttagtatgat aaatgatggt caactctcag atggaggatt
     5161 aaataagatg attttccaat ctgctgaaag gtctcgtgca tcttacttag atactgcaaa
     5221 aatagtttca cgaataggca tgaacgcagg taaggcgttt agcagtacaa aagaaattgt
     5281 aggttttgca gagcaactaa ataaaaagtt cgtaattgct ggagcaagta ctgaggaaat
     5341 gaattcggca ttgttacagc taacccaagg gttgagttct ggtgtattaa gaggtgagga
     5401 actgaatgct gtgtttgagt cagcacctaa catcatccaa tcgattgcag attatttgga
     5461 cgtggacata ggaaaaataa gaggaatggc atcagaggga atgttaacag cagatattgt
     5521 aaaaaactca ttacttgcag cagcagagca gactaatgca gagttcgaga agatgcctta
     5581 tacgctaggt caaatcttta ctagtgtaaa aaataatgca gttatggtat ttggagctat
     5641 acagaagaaa attgaggaca cagtttcaag caggggattt cgaactttca taactgatgt
     5701 taaagactca ttatatgtac ttggagcagt tgggtttaat gtatttagtg gatttattaa
     5761 tttactgagt agtccagctt ttcagaattt ttttaatgtg atgattgttg gaactagttt
     5821 agttgtacaa gggctaggtt ggataataac acaagcactt agtgttgcta atgtattttc
     5881 gcgaaattgg agtataattt cacctataat tttaggtgta gtttcagcta ttagtaccta
     5941 tataatagct cttggtatta tgcaagcagt tactatagct ggaactgtag caaattggat
     6001 gtcgtggaca tctgaagtta ttagacaaag aggaatttta ggaacagcta gagctttagc
     6061 gataggcaaa gctgaacaat ggggatttaa tgctgctata tatgcatgtc ccattttttg
     6121 gatagtttta ggtataattg cagtaatatc tgctgtattt atattagtag cagtttacaa
     6181 tcactttgca ggcacaagta tttctgcaat cagtcttata gttggagctt ggtattggtt
     6241 atgtgcagtt gtttacaacg taattgtagg gatagtaaat gctataaata tctgtgtggt
     6301 tgaaatagca aatatcttta gaactggatt atatgcagtg caatgtttct ttattgatat
     6361 ggctaatgca ggactcaagg caggtgttaa tttagacaaa gcttttgata aatttgctac
     6421 caatctagca aatggtatta taaaagctgt taatatagct gtaaaaggat tgaattggtt
     6481 agtacaacag attaataaaa ttccaggaat tgatttacca caaatgaaag aatttcaaaa
     6541 agtaaatact gtaattggta caaaaacaac atttaaaccc attcaaaaac cgcctgagcc
     6601 taaagcatgg aaaccagaat tagtagagta caaaaattta aagtcagaat ttataaaagg
     6661 atatgacgta ggacatcaat tacaaaacaa atttaaagat acttttgaca ttagcaagat
     6721 agccgaaaaa gcaaaaaaag atttaggtct agatgacctt tgggataaaa aatacggact
     6781 tggtgatgga ttgggttcag ctggattaaa ttcacctttg agtgacgcag caaaaggagc
     6841 aaaggacaca gcaggcaata ctgcaaaaat ggcaaagact atggataaaa gtcaagagga
     6901 cttaaaatat cttagagaca ttgcagagca agaaacaata aaccgattca caggggtaaa
     6961 tataaaaatt gacatgaaca acactaataa cataagtaaa gatgcagatg tggatggtat
     7021 agttaatgta ctaacagaaa aactgaatga tgctatggtt gtatctgctg agggaatagt
     7081 ttagagagga ggtgtaatcg tggcttatga tttttattta gatggagtac aattaccaat
     7141 acctccacca aagttagaga ttaaagttac aaataaaaat aagacagttg atttgataaa
     7201 tactggagaa gtaaacatat taaaaaaaga agggctatct gaaataagtt ttgaagcaga
     7261 gtttacacat aataaattac ctttttatcg tggaactttt agggatgttc aattcttttt
     7321 aagtaagcta gaattactaa aaactgattg taagccattt caatttattg tatcgaggga
     7381 aatgggtgga aaagtactat ttaacactaa tatgaaagtg tcattagaag aatataacat
     7441 agtagaagat gcagacaatg gttcagatgt taaagttgca ataaagttaa agcaatatag
     7501 agattactca actaaaaaat tagttcctgc aactcctgaa aagacaaact atggtaggac
     7561 tcccactcca gtcatgaaac caaaagaatt tagaccagat tcatccaata agccaaatgg
     7621 taaaacatat acagtaaaag caggggatag cctttggtca atctgccaga agcaacttgg
     7681 taatggttca ttatataaga aagtatatga gttaaataaa acaatgatgg ataaagctaa
     7741 caagggtaaa aaagtaccta aatacacaat ttacaaaggg caggtgttaa aacttggctg
     7801 atgaattagt tctggcaaat gatagggatg taaggctagt tattgcacat tgggaagatt
     7861 tctacgaacc agttgtcctt gatggtatca catgggagat agaaagaaga ggaacacctt
     7921 ctaagttaga atttacaata gttatggatg atatattaga gttttgcgaa ggaaattctg
     7981 taaggctgta ttataaagga ataggtatct tctatggata tatatttcag aagaaaagag
     8041 ataaagaaaa tcacattaaa attgttgctt acgaccagct aagatatttt aagaataaag
     8101 atacttatgt atatagtaat aaaactgcaa gtgaacttgt aaaaatgttg gctaaagatt
     8161 ttaatttaaa atacaatgtc atagaagata ctaagtataa aatatctagg atagaagaaa
     8221 ataaaacact ctttgacatg atactaacag cactagatga tactctaaga gagaaaaagg
     8281 aaatgtatgt tttatatgat gagtttggaa gaataacatt aaagaatgtt gcatcaatga
     8341 aacttgatac ggttatgaac aatgatgtaa ttgaggattt tgactataat tcatcaatag
     8401 atagtgatac ttacacaaag attaaacttg tcagagacaa cgaggagtca ggaaaaagag
     8461 atgtgtatat tgcacaagat tctacacata tgagaagttg gggaatacta caactatttg
     8521 atacagtaga taaaaacatg agtgaagcag agataaagca aaaatgtgat atacttctaa
     8581 aactatataa taagaaaact aagtcattaa gtttaaaaaa tgtgttagga gatattagag
     8641 taagagcagg ttgtttagta cctgtttttt tagatttagg agatattgat ttacaaaatt
     8701 atatgttagt tgagaaagta aagcacacat ttgaaaataa ttcgcacttt atggatttga
     8761 ctcttgttga tggagatgaa tttgcttctt attcttcaag ctcatatagt agtggaaata
     8821 ctaataataa agatgaaaag aaaaatggtc ctgcacaaag tactacaagt aaagaagaca
     8881 atgatatgat aaataaatta aataaagtat ttaaaaataa gttatcaaat acaggaagta
     8941 tatttgttaa atattctaat gcttacaaag tcaatgcagc tttaatggct gctatttcta
     9001 tacatgaaac tggtaatgga agttcttcac tttgtaaaaa taaaaataat ttctttggaa
     9061 tgaaaggaat gtcttttggc tctgtggatg aaggaataaa aagaggtata agtaatttat
     9121 caagaaacta tatccatatg ggaagaaaaa cattagaaag tattagagac aaatatgctc
     9181 cactttatga tagtcctctt aataaacatt gggtaccagg agtaaataag ttttataaac
     9241 aaataacggg aaatgcatat agttctaata gtgcaggtac aggtgttaga agcaacgagg
     9301 aagcagaaaa gaatttaaaa gatataactt atcaagttca aaatagcaac aatgctaata
     9361 catcaacaaa caataatagt aaagtaaata aagttattca agaagcaaaa aatcaacttg
     9421 gcaagcctta caaatggggt ggtaatggtc caaagagttt tgactgtagt ggtcttatgg
     9481 tgtgggcatt caaaagaggt gcaggtataa atctcaaaag agtttcagca gaccaatcaa
     9541 atgatagtag aggaaaacta ttatgcaaca taaatgatgt aaaagctggc gatttagtat
     9601 tctttgcata caaccaagga aaaggaaatg tacatcacgt tggactatgt ataggaaatg
     9661 acaagttcat acattcacca aatactggtg atgtagttaa aataagcagt ttaagtggta
     9721 gacaaaaaca aaaacatgat tttgcaagag ctagaagatt cttttaagtg aggtgataat
     9781 atgagccaag atttattaca aataataaag aaagctgcaa tggatgcagt agaaacaagc
     9841 aacccaatga gggttgtatt tggaacaata gaaagtatta atcctctaag agttaagata
     9901 gaacaaaaac tatctattgg tgaaattttt ctaatacaaa cagatacatt taaaagatat
     9961 acagataaaa aaataggaga taaagtagtc ttaattcgta tgcaaggagg acaacaatat
    10021 ttagtattgg ataggatgtg atgaagtgtt accaagcgat aatttagatt atgacattga
    10081 agatgtatca ataattaatt ttgatgtaag gcaagaacca agtaagacct ttaaattaaa
    10141 tatagaaaaa tctaagatag atggtatttg tgatgatgtt gaagcattaa aacaaaccat
    10201 ctttttaatt ttaaacacag agagatacca acatctaata tatagttgga attatggagt
    10261 cgagttgaac gaccttattg gagagcctat atcctttgta atccccgaac ttgaaagacg
    10321 aatcaaagaa gcactaattc aagatgatag ggttgaaaat gtagataatt ttgagtttca
    10381 aaatgtaaag ggtaaagtac attgtaagtt cacagtttac agtaaatatg gaaatataaa
    10441 agcagagaag gtggtgagtg tataattgtt tgagttaatg acatttgaaa atataattaa
    10501 aagaatgtta gatagtgtac cagatacttt tgataaaagg gaaggttcta taatatataa
    10561 tgctcttgct cctgttgcta tagaacttac agaaacatac attgccatgg atgaattact
    10621 agaccaaact tttgtagata ctgctagtta ttactattta gagaagagat gtaaagaaag
    10681 aggtattaca cctttacctg caaccaatac aattgcaaaa ggagttttta atatagatat
    10741 tcctattgat tcaagattca acttaggaga atacaattat gtggcaattg agagaatatc
    10801 tgaaaaaaca tataaaatga aatgtgaaac tactggacct gtatttgaac ttggtcagtt
    10861 gattccaatt gaatatgtag acaaattaga aactgctgag ttaactgaaa tactaattaa
    10921 tggagaagat gaagaaagtg aagatagttt aagacaaaga tattatgata gcctaaactc
    10981 acagagtttt ggtggaaata tgcaaaatta taaagatgaa gttaacaaaa tacaagatgt
    11041 tggaggagtt aaggtttatc ctgtgtggga cggtggaggt actgttaagt tagtaataat
    11101 taattctaac ttcaaagttc catctagtga tttagttaat ttagttcaag aagaaattga
    11161 tcctctacaa aaccaaggag aaggtcttgg attagcacca attggacacc gagtcacagt
    11221 tgaaggagtt acaagtacaa ctataaatat atcagcagag ataacataca aaaatggcta
    11281 cacttgggag aatataaaat caattgcaga agaagcaata gacgactatt taaatgaact
    11341 taatatgagt tgggaagatg aagaaaactt aatagttcgt atatctcaaa ttgaaactag
    11401 attacttagt attgatggag tattagacat tgcaaataca atgataaata gcattgcatc
    11461 taatcttaat gttgataaag attctatagt attaagaggt gaagtagttg gataaagaga
    11521 ttaatctaat aaattactta ccgcaaatcc tacgagataa agaagaatat ataaaagtat
    11581 ttaatgtaga aaacaaagaa ataaaaacac tacatgaaaa attaaatgac ctatcaaatg
    11641 accagttttt agaggattta actccaagtg gcataaaaag atgggaaaag ataatgtcta
    11701 taactcctaa aagtaatgag agtttagaag atagaaggtt taggattttt agtaaatata
    11761 taagtaaatt accttactca gagagatttt taaggaactg gctagataat gtagttggag
    11821 aaggcaatta tgagttaact at
//
LOCUS       DAYAOY010000118        11777 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYAOY010000118 DAYAOY010000000
VERSION     DAYAOY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.118"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(348..893)
                     /locus_tag="ACGI6B_003426"
     CDS             complement(348..893)
                     /locus_tag="ACGI6B_003426"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9752839.1"
                     /translation="MRVEPIENKKDIEKLKRYFKNKGKIRDLTLITLGLNIGFRIEDI
                     LKLKVKDVYKKREINIKESKTGKRIRKPINDSLKEILDIYCKDKPTNEYLFKSQKGEN
                     EAISTTQAYRIIKKASKACRIKLNIGTHSMRKTHGLHIFKDKGIEVAQRALNHANQID
                     TLTYIGVVQKQADDAIMKLNL"
     gene            complement(909..1157)
                     /locus_tag="ACGI6B_003427"
     CDS             complement(909..1157)
                     /locus_tag="ACGI6B_003427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752840.1"
                     /translation="MEGTKRSYRKIPKLDLRKVKLADVFEKNEPGVFRVKESDEQLLY
                     DCLEETQNIRQEKHIAYAIRFRGKSVYCVFYESPFWDL"
     gene            complement(1513..2148)
                     /locus_tag="ACGI6B_003428"
     CDS             complement(1513..2148)
                     /locus_tag="ACGI6B_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9752841.1"
                     /translation="MIIGYERVSTRKQDNSRQERVLENEGIKFDKIYKDVITGATKER
                     EALSRMLVEVNKGDTVYCESISRLGRNLKDLIDIIDTLVQKGVRVKILKEGIDTNNNT
                     YKMLLGIFGAVAEMERETIQERTQQKIDQLKEDKKNTGEINTKSGKWFGRQKVTKELL
                     ETNKEFIKYYNKILDGSLKKNEVAKILNVGRTTLYEWIKIYEGGEIKRNRS"
     gene            complement(2738..3106)
                     /locus_tag="ACGI6B_003429"
     CDS             complement(2738..3106)
                     /locus_tag="ACGI6B_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752842.1"
                     /translation="MELDFKFIGQRIKIARIKKKLTQEVLSEKINVSPQHVSNIETGN
                     SSVSLPTLVAIANTLGVSVDELLCDTILSSKAVFENELEEVLRDCNEYEIRFITDIVK
                     ASKKSIRDIKLFQSNLNDRY"
     gene            3222..3554
                     /locus_tag="ACGI6B_003430"
     CDS             3222..3554
                     /locus_tag="ACGI6B_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9752843.1"
                     /translation="MDIPKIPQTELKVMKFIWSKNDIVTSKEVAKAMELEYSWKMTTT
                     LTILSRLVIKHFLASERIGRMTHYTILIAKKDYKISETKRFLNDIHSNSLESLLNSLK
                     DCNITHKK"
     gene            complement(3702..4139)
                     /locus_tag="ACGI6B_003431"
     CDS             complement(3702..4139)
                     /locus_tag="ACGI6B_003431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752844.1"
                     /translation="MRVKNIISIFVCALIIIMSVNVVSFAEQFNSSDIIFMEDGKEEI
                     NPFSVYILNGRCNLSFSNGKANVNASVVGKSGVTRTSIVTKLQELKGGSWVNLKTSTK
                     KTGGKTCSVLNSYSVTKGHTYRAVAIITAENESKTLISTSQKY"
     gene            complement(4208..4909)
                     /locus_tag="ACGI6B_003432"
     CDS             complement(4208..4909)
                     /locus_tag="ACGI6B_003432"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HFG9752845.1"
                     /translation="MNKDKLDIEKLLYEQLPKIEEKILYELPCDEDIDYEFSIEFESK
                     MDKIIKENEKKVKKNTYKNLKKIAIVFIIILSSIVTITTIKTEAFPENLIRIMKKVYK
                     EFTDYNFINPHKSNVSFKYIEPKYISTGYKEVERIEEENYLSILYKDKLDNYIIYQMM
                     LYSRDVIVLDTEDAFVENIKFNNQYAQYVEKGNTQQLLWHDKNNIYMLSKELPTKKIT
                     KKDKKEFIKIAKNIK"
     gene            complement(4902..5447)
                     /locus_tag="ACGI6B_003433"
     CDS             complement(4902..5447)
                     /locus_tag="ACGI6B_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9752846.1"
                     /translation="MLIYLAIIDSKEERIKFEEIYIKYKKLMFYVANQILKDDVLAED
                     AVHSSFLKIIDNLDKVNDVNSPKTKGFVVIIVKRISINIYNRRKKEEISNINDDNYKF
                     SSLDLSIEKLGECSYLGEALKKLSETDLQVILLKYSHGFSIREISKLLNIKEVNLYKR
                     NKRALEKLKKILSEMEALSNE"
     gene            5583..6311
                     /locus_tag="ACGI6B_003434"
     CDS             5583..6311
                     /locus_tag="ACGI6B_003434"
                     /inference="COORDINATES: protein motif:HMM:NF039973.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752847.1"
                     /translation="MKSKRINKMLATTMTMAMLAIPAVTATSYAMESTGLGTEYTYNN
                     ESKQQEEWVKSFDTETGKEILLNKTTNEKVSEVYENVNGKLVKLSINEALEKFNLNEE
                     IITKSPKSIFGVSSVKKITGSARKISQDVSGGKQGATISIGNSTTVTESFTVGGTTEA
                     IKAAVRANAGFTWSKSLTRSVTTTHKVPAGKIGYVAFKPYYKEVRGTVNGILAGTSIT
                     QKIVAKSPLKAGKVCDGLEYLAYK"
     gene            6337..6585
                     /locus_tag="ACGI6B_003435"
     CDS             6337..6585
                     /locus_tag="ACGI6B_003435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752848.1"
                     /translation="MENKNMYLRVSLILAVLGFIILFFNNDLALSLKATYLADKGFED
                     IVENQILKNYSYMFLIIGGVLFSIGIYNLTKLKQINKK"
     gene            complement(6771..9380)
                     /locus_tag="ACGI6B_003436"
     CDS             complement(6771..9380)
                     /locus_tag="ACGI6B_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9752849.1"
                     /translation="MKIDIKQLIDIIKNANIDELYLRDIGVDTTQKRNVPCPIHGGKD
                     KNFQFDLNKRIYTCYSHNCVVGADIIELCRTYERFERPIEAILFLANKYNISLPINKS
                     KTNIKKTNNTIKNIYIKKKDINSFIEEKKQEAFKNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYKADSIINIDKYISENLKGIRESIKHATEGKNVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYKIAGAYDKIPTKHALEGNKLVAMTW
                     DKTEQLLKEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYDKINIGKVIEILNNSNNSMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVITAGVNINNPNVTDIIVIGE
                     KDVGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPYRAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRTI
                     NSFKVLLEEYFNKIEIVETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKYYMEHDLYSLIINNNCRKTIDLFSDFVLDN
                     SYSIDLSWKLATMSDEKRNLFFEQINTTIYRKINKKYANSLLNDDNIGTYTYNYINFN
                     FGVGTSYTKEHLEALAVDLQTFLATKKKLTIKKISLILQSIFVIEVKQHRGVTGLPYK
                     YYKNILPNPVTDKKVIRVYTIKKHIDIEDIKKELGLIDNDLSIEHLVEARINKLLNAI
                     DETEKEVLLEGLI"
     gene            complement(9710..10024)
                     /locus_tag="ACGI6B_003437"
     CDS             complement(9710..10024)
                     /locus_tag="ACGI6B_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752850.1"
                     /translation="MLKVMLKDDLLRIGFDTYGVNLEDKLLENNEIYLNDKKESERIM
                     NRLLEILEEKEHKKMLIDFEEKVFGNSVIEKEYAYRMGVLDGLDLAEFIDLNKKLCLK
                     RN"
     gene            10166..10384
                     /locus_tag="ACGI6B_003438"
     CDS             10166..10384
                     /locus_tag="ACGI6B_003438"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752851.1"
                     /translation="MAIKLLDIQINKLLENQNKTRYWLAKQIGMTHQNLTKLANNNTN
                     SIKFDSLEKICMALKCSPNELFGWEQDK"
     gene            10464..11009
                     /locus_tag="ACGI6B_003439"
     CDS             10464..11009
                     /locus_tag="ACGI6B_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752852.1"
                     /translation="MDRYTFDNLPIKEQIQYINNKLLEGNTLTNTCKDIGIGRTTIRN
                     RFKQHGYEFNPTKKLYISIIEVVELESSSKVVAGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLNIDDFKGNIVVRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALRDFMDKYK"
BASE COUNT         3901 a         1821 c         1281 g         4774 t
ORIGIN      
        1 aaaaaaaaat aaccatacgc attgatttta cttaaattaa aagtgttttt cgagttgttt
       61 tttcacttag tttagtaatt ttgaaatatt ataatattaa gcttcaatat gtatctatta
      121 tcatttaata atcaattaat aatagatatt ttttctaaac aagataaaaa caatatctat
      181 accagtaata gcaatagata gaagtatata aaaaaatgta cgattactat aaactattac
      241 atttatttta gttatcttta tccattgcaa ttacttatac ataagcttat ttacttaatc
      301 attaactata aaatgtaaaa acaacaaaga aaatcataca tttttaacta taaattaagt
      361 ttcataatgg catcatcagc ttgtttttgt actaccccaa tataagttaa tgtatctatt
      421 tgattagcat ggtttaaagc tctttgtgcg acttcaattc ctttatcctt aaatatatgt
      481 aaaccatgtg ttttcctcat gctatgagta cctatattta attttatcct acaagctttt
      541 gaagcttttt taataattct atatgcttgt gtggtgctta ttgcttcatt ctctcccttt
      601 tgacttttaa ataagtattc attagttggt ttatctttac aatatatatc taatatttct
      661 ttcaaactat catttatagg ctttcttatt cttttccctg tcttactttc ttttatattt
      721 atttctcttt ttttgtatac atctttaact tttagtttta aaatatcctc tatcctaaag
      781 ccaatattta accctaaagt tattagggtt aaatctctta ttttaccttt atttttaaaa
      841 tatcttttaa gcttctctat atctttttta ttttcaatag gttctactct catttaactc
      901 actcctattt ataaatccca aaatggactc tcataaaaaa cacaatatac actttttcct
      961 ctaaatctaa tcgcataagc aatgtgcttt tcttgtctaa tgttttgagt ttcttctaaa
     1021 caatcatata agagttgttc atcactttct tttactctaa aaactcctgg ctcattcttt
     1081 tcaaatacat ctgctaattt tacttttctt aaatctaatt ttggtatttt cctataactt
     1141 cttttagttc cttccataat ttcccctctc atttttctat ttatataaat tattttaata
     1201 tatttttaca atccaaaagt ctctattttg ttcaaaatat gtagtttttt attacttgtt
     1261 aatttacaaa atcatttgat gtgattttat cattctatct actataccaa aaaaaatctt
     1321 attttcttaa gtaaatcaaa atttaatatt tataataaaa aaaagagcct aaaaaattag
     1381 actcttgttt gcaaaagcta ttaaattgta aagctttcaa taatataatt atactatata
     1441 ttttgttctt attttgtcta attttgttca atttttgttc aaaataataa cacttaaata
     1501 tattataatc aattagcttc tattcctttt tatttctcca ccttcatata tctttatcca
     1561 ctcgtaaagt gtagtcctac caacatttaa tatttttgcc acttcattct tctttaagct
     1621 tccatcaagg attttattat aatattttat aaactctttg ttagtttcta aaagctcctt
     1681 cgttactttt tgtctaccga accattttcc actctttgta tttatctcgc cagtattttt
     1741 cttatcctct tttagttggt ctattttctg ctgtgttctc tcttgaattg tttctctctc
     1801 catttctgca actgccccaa atataccaag taacatcttg tatgtattat tatttgtgtc
     1861 tataccttct tttaatattt ttactcttac acctttttga actaatgtat ctataatatc
     1921 aattaaatct tttaaattcc ttcctaaacg gcttatagac tcacagtaaa cagtatcccc
     1981 cttatttact tctactaaca ttctgcttaa tgcttctctt tcctttgttg ctcctgttat
     2041 tacatcttta tatattttat cgaacttaat tccctcattc tctaaaactc tctcttgtct
     2101 gctgttatct tgttttcttg tacttaccct ttcatatcct attatcataa ccctaccccc
     2161 atttgtcttg ttataataat tatacaatat attttttgtt cggtcaaact atatttttca
     2221 tttttgcaat gccgaacata ttttattgtt gatttccaat gattctatgt aaaaatcata
     2281 caaatattgt gtgtacggtc aaacgttggt ttttccgaac acgaacatat cacctttttt
     2341 atatcaacta ttccttaaat attttatata aaatcgccaa aatcttttta tattttttag
     2401 gcaaccaagt ttttaaaaca aaaatggtat atcagtaaga attatgtttt cacacaatat
     2461 aattcttaat gatataccat ttaagaacct cataagaggt agatagagat aattacataa
     2521 tcatcacata cgttttatca aatctgtttt gtaatagggt ttatacttaa ataataacaa
     2581 aaataccgta ttttttctac atttatactt tttttgttga tagtaaaata tggaaattag
     2641 tcatattttc agttattaac tcataagttt atacaacttt tatttaaata taataaaaac
     2701 atcaataata attaaattat tgaagcatat atttatatta atatctatca tttaaatttg
     2761 attgaaacag ttttatatct ctaatggatt ttttagaagc ttttacaata tcagttataa
     2821 atcttatttc atactcatta caatccctca aaacttcttc taactcattt tcaaagacag
     2881 ctttagaact taagattgta tcacataata attcatcaac cgacacccca agagtatttg
     2941 caatagcaac gagcgttggc aaacttacac ttgaatttcc cgtttcaata ttgctgacat
     3001 gttgtggact aacattgatt ttttctgata aaacttcttg tgttagcttt ttcttgattc
     3061 ttgcaatttt tatcctttga cctataaatt tgaaatcaag ctccatatgc gtattccctt
     3121 cctacaagta ttcacacata ttgtaacctt tcccaaagta tattataata aattaataga
     3181 atgtttcatt atgatagtta tagtgtatag aaaggaatta tatggatata ccaaagatac
     3241 ctcaaacaga actcaaagtc atgaaattta tttggagcaa aaacgacatt gtaacatcta
     3301 aagaagttgc aaaagctatg gaacttgaat atagttggaa aatgacaaca accctaacaa
     3361 tcttatcaag gcttgttatt aaacactttt tagcatctga acgaataggc agaatgacac
     3421 attatacgat actcatagca aaaaaagatt ataaaatatc tgaaacaaaa agatttctaa
     3481 atgacataca ttcaaattca ttggaaagcc ttttaaactc tttgaaagat tgcaatatta
     3541 cacataaaaa atagtacatg agtataaatt atattattat acctatgtac tatttaagtt
     3601 tttctataat gttcctatat ctatctaatt ttgattgatt tagatacttc tgtagtagtt
     3661 ttcaaaacgt ttcttaactc ttgtaagtgt attttctttt tttaatattt ttgagatgta
     3721 cttattaatg tttttgactc attttcagca gtaattattg caacagctct ataagtatgt
     3781 ccttttgtca cagaatatga gtttaaaaca gaacaagttt ttcctccagt tttctttgta
     3841 gatgttttta aatttaccca acttccgcct tttaattctt gtaacttagt tacaatagat
     3901 gttctagtta ctccagattt tccaacaaca gaagcattta catttgcttt tccattagaa
     3961 aatgataaat tacatctacc atttaatatg taaactgaaa aaggatttat ttcttcttta
     4021 ccatcttcca taaaaataat atcagatgaa ttaaactgtt cagcaaaact aacaacattt
     4081 actgacatta ttattatcaa cgcacataca aatattgaga ttatattttt tactctcatt
     4141 aagtacctcc ttataaaatc ttatatattc aatataacaa ctaactagca ctattttaga
     4201 caaaaattta cttaatattt tttgcaattt taataaattc ttttttatct ttcttagtta
     4261 ttttttttgt aggtagctct ttagataaca tataaatatt atttttatca tgccacaata
     4321 attgttgagt atttcctttt tcaacatact gtgcatactg attattaaat tttatattct
     4381 caacaaaagc atcctctgta tccaagacta taacatccct agaatataac atcatttgat
     4441 aaattatata gttatctaat ttatctttat ataatattga tagataattt tcttcctcaa
     4501 ttctctcaac ttctttatat ccagtagaaa tgtatttagg ttctatatat ttaaatgata
     4561 catttgattt atgagggttt ataaaattat agtctgtaaa ttctttatat accttcttca
     4621 ttattctaat taaattttct ggaaaagctt ctgtttttat agtagttata gtgactatac
     4681 ttgataaaat tattataaaa acaatagcaa ttttttttaa gtttttatag gtgttctttt
     4741 ttactttttt ctcattttct tttataattt tatccatttt actttcaaat tctatagaaa
     4801 attcatagtc aatatcttca tcacaaggta attcatacag tattttttct tctatttttg
     4861 gaagctgttc gtataataat ttttctatgt ctaatttatc tttattcatt tgataatgcc
     4921 tccatttcgc ttaaaatttt ttttaatttt tctaatgctc ttttatttct cttatacaaa
     4981 ttaacctctt ttatattcag tagtttagaa atttctctga tagaaaaacc atgactatat
     5041 tttaagagaa tcacctgtaa atctgtctct gacagttttt ttaaagcttc tcctaaataa
     5101 ctacactcac ctaacttttc aatcgataaa tctaaagagc tgaatttata gttatcatca
     5161 ttaatattac ttatttcctc tttttttctt ctattatata tatttattga aatccttttt
     5221 actataataa caacaaaacc tttagttttt ggactattca catcatttac tttatcaaga
     5281 ttatcaatta tttttaaaaa tgaactatga acagcatcct ccgctaaaac atcatctttt
     5341 aaaatttgat tagctacata aaacattaat ttcttatatt taatatatat ttcttcaaat
     5401 ttaattcttt cttcttttga atctataata gctaaatata ttaacataaa ttattctccc
     5461 ttaaaaaata ttatccttaa aaaaattata tacaatttat aaaaagttat atataaatgt
     5521 ctaaaaataa aaacttcatt tgttatatat ataaataaaa acataaaagg atggtgtttt
     5581 atatgaaatc aaaaagaata aacaaaatgt tagcaactac aatgacaatg gcaatgctag
     5641 caataccagc agtaacagca acatcatatg caatggaatc aacagggtta ggaacagaat
     5701 atacatataa taatgaatct aaacagcaag aagagtgggt taaatcattc gatactgaaa
     5761 caggaaaaga aatattatta aataaaacta ctaacgaaaa agtttctgaa gtatatgaaa
     5821 atgtaaatgg aaaattggtt aaattaagta ttaatgaagc actagaaaaa tttaatttaa
     5881 atgaggaaat tatcacaaaa tctcctaagt ctatttttgg agtatcaagt gttaaaaaaa
     5941 ttactggttc tgcacgcaaa atatcacaag acgtatcagg tggaaaacaa ggtgctacta
     6001 tatctatagg aaattcaaca actgtaactg aaagttttac tgttggtgga actacagaag
     6061 caataaaggc agcagtaaga gcaaatgcag gatttacatg gagcaaatct ttaacaagaa
     6121 gtgtcacaac tacacacaaa gtacctgcag gtaaaatagg atatgtagca tttaaaccat
     6181 attataaaga agtaagaggt acagtaaatg ggatacttgc aggaacttct ataactcaaa
     6241 aaatagtagc aaaaagtcct cttaaagcag gaaaagtttg tgatgggtta gaatatcttg
     6301 catacaaata ataaataata gaaagagttg attaaaatgg aaaataaaaa tatgtactta
     6361 agggttagtt taatattagc agtattaggc tttataatat tattttttaa taatgactta
     6421 gcattatcac taaaagcaac atacttagct gataaaggct tcgaagacat agttgaaaat
     6481 caaatattaa aaaactattc ctatatgttt ttaataatag gtggtgtact attttccata
     6541 ggaatctata acttaaccaa actgaaacaa attaataaaa aataaataaa ataaaaggac
     6601 ttataaatta taagtccttt tattttacac ttataagcta ctttcaacct ttcatgattt
     6661 aacctttatt tatacaacac ccaaaactcg acctcaaaaa gagaacagga cttcgtcccc
     6721 cttaatacaa agcaacattt tttagtacta ttcgtactta agattagcta ttagattaat
     6781 ccttccaaaa gaacctcttt ttccgtttca tcaatcgcat ttaaaagttt atttattcta
     6841 gcttcaacta aatgctctat gcttaaatca ttgtctatta gtcctaattc tttttttatg
     6901 tcctctatgt ctatatgttt cttaatagtg taaactctaa ttactttttt atctgtcaca
     6961 gggtttggaa gtatattttt atagtattta tagggcaacc ctgtgacacc cctgtgttgt
     7021 ttgacttcaa taacaaaaat tgattgtaat attagactta ttttcttaat agttagcttt
     7081 ttcttagttg ctaaaaacgt ctgtaaatca acggctagag cttctaagtg ttcttttgta
     7141 taacttgtac caactccaaa attgaagttt atataattgt aagtataagt accaatatta
     7201 tcatcattta gcaaagaatt tgcgtatttt ttattaattt ttcgataaat tgttgtattt
     7261 atctgttcaa aaaatagatt tcttttctcg tctgacattg tcgcaagttt ccaagatagg
     7321 tcaatactat aactattatc taatacaaaa tcactaaata agtctatcgt ttttctacaa
     7381 ttattattaa ttattaggct atataaatca tgttccatat aatattttaa acagatatta
     7441 ttatctatcc tcatgtcatg atgatatttt aataagttac tgctggtcat tgacttctta
     7501 atttcactat agccaactag atacttttta tgtccttcta gttgttctat agctgatttc
     7561 ttaaattcct taatagcttc ctcatgctct tttatttctt cctctatttt tttagtttct
     7621 ttttcagttt ctactatctc aattttatta aaatattcct ctaatagcac tttgaagctg
     7681 tttattgttc ttgtttggta atagttattg taagcattac tttttatgta taacttgtcc
     7741 gttttatagc acatatcttt tttatcatag tatatattac tatcaatgtt ttttatgttt
     7801 atagggctta tatcaattcc tatagctcta tatggtaagt ctctcttaga agctttattg
     7861 tatatatttg ttaggctctc aacgtctttt atgttctcat ttatgcacca ttcaaccgaa
     7921 tatacattgc tatcctcatt tgtcgttttg taactattaa aaatatgtac atttatactt
     7981 tttgcccctc tagctcttgc aacatactgt tttatttttc ctacatcctt ttctccaatt
     8041 acaataatat ctgttacatt aggattattt atgtttacac ctgccgtgat tacagacgta
     8101 tttaaaagca cctcatagcc ttccatatcg gagtaactaa caattctatc atatagtttg
     8161 ctagtgtctt tagtatctga aataacaact ccactctttt tattaacttt ctttgatata
     8221 tagttaagtg ttgatatact atcttttaaa agcattgaat tatttgaatt atttagtatc
     8281 tctattactt tcccaatatt gattttatcg tataacttaa ctttgtattc cgtttgtata
     8341 ttttgtttat attcaataac tttgttatat atctcaaatt ctaatttaga gggtgttgca
     8401 gttatatcta gtctacctct gcacttatta gttatattat ttaagctctt tattgctttg
     8461 cttctgaacg catctgtgta agtctgatgt atttcatcaa taactattat gtactcgctc
     8521 aaatcctctt ttagaagctg ttcagtctta tcccacgtca tagccactaa cttgtttccc
     8581 tctaaagcgt gtttagtagg tatcttatca tatgcaccag ctattttata ttcgactatt
     8641 gcttgttgca cattcgcaga gttggggaat ataaagagtg cttttatatc cagctctttt
     8701 aatgtattta taattgagta tgtttttcca cttcctgtag gagctaccat taatacgttc
     8761 ttaccttctg tagcatgttt aattgactct ctaatacctt ttagattttc acttatgtat
     8821 ttatctatat taattatcga atcagcttta taacttgaat agttttgttt ttctagcatg
     8881 gattttattt taatattgtt atctaagtaa tactttcttt tgtccttatc agtcatacac
     8941 tcaaattcaa aagctaactc aatatcattg tttttaaaag cttcttgttt cttttcttct
     9001 atgaagctat ttatatcttt tttctttatg taaatgtttt taatagtatt atttgttttt
     9061 ttaatatttg tcttactctt gttaattggt aatgagatat tatatttatt ggctaggaat
     9121 aaaatagctt ctataggtct ttcaaagcgt tcatatgtgc gacataattc aattatatca
     9181 gcaccaacaa cacaattatg gctatagcat gtatatattc ttttgtttaa atcgaattgg
     9241 aagtttttat ccttaccacc atgaattggg caaggaacat tccttttttg agttgtatcg
     9301 actcctatat ctcttaaata tagttcatct atattggcat tttttattat atcaattaat
     9361 tgttttatat ctattttcat ttgtaaaaat cccccttttt tagttagggg ctaatcaagt
     9421 tttatcaagc tatgtattta cattctgccg taaatctcgt ataattaaca tataaaagaa
     9481 taaggaaata tatattttat atgttaatta agcctaagtg attagctcct agtattaacc
     9541 cctagtggct aactcctaat cttgattaac ccttaaatgg aaagaatgag tggatgccgt
     9601 caaacaaata ctcgttcttt ttcgtttttt ttgttattag tattatatca ctattaaagt
     9661 tataattcat tgtttagaat tatttttctg acggttttaa tatatatttt taatttcttt
     9721 tcaagcataa ttttttattt aagtctataa attcagctag gtcaagccca tctaaaaccc
     9781 ccattcggta tgcgtattct ttttcaatta cactgttacc aaatactttt tcctcaaaat
     9841 caattagcat ttttttatgt tctttttctt ccaatatttc taacaatctg ttcattattc
     9901 tttcagattc ttttttgtca tttaaatata tttcgttatt ttctagtagt ttatcttcta
     9961 aatttactcc gtatgtgtca aatccaattc ttaataaatc atcttttaac attactttta
    10021 acactccttt ttatgcacac ttaaatcttt tgcaccccta aatatggtaa tatattctat
    10081 ttattattat gtaattattt actatgtatt gtcaagcata tatattgttt ttggtacata
    10141 tttccgatac tatataataa aaaaggtggc gattaaattg ttggatatac aaataaataa
    10201 attattggaa aatcaaaata aaacacgtta ttggttagca aagcaaattg gaatgactca
    10261 tcaaaactta actaaactag ccaacaataa tacaaattct attaaatttg attctttaga
    10321 aaaaatttgt atggctctaa aatgttctcc taacgagctg ttcggttggg agcaagacaa
    10381 ataaaaaaat ataaatccta aagggtttat ttataactac tagaaaacta ctttagtagt
    10441 tattttattt tggaggttta aacatggata gatatacatt tgataactta cctatcaagg
    10501 agcaaataca atatattaat aacaagctct tagagggcaa tacattaact aacacatgta
    10561 aagatattgg tattggtcgt acaactataa gaaatagatt taaacaacat ggatatgaat
    10621 ttaatccaac taagaagcta tatatatcta ttattgaggt tgtcgaatta gaaagtagtt
    10681 caaaagtagt tgcgggcgac actagcaatg acaatacttt agttgatgca accaatattg
    10741 attttaataa cattttatct aaattcaatg atatgaataa taaactaaat gaagtatata
    10801 gttggtacga atcacaaagt agtaataaag tagttggggc tgataaacta aatatagacg
    10861 attttaaagg caatatagtt gttagaagct ataaggtcta tgaggatatt caaaaagagt
    10921 ttgtagcctt ctgtgaagct aataagaagt acagggtaca agatattatc agccaagctc
    10981 ttagggattt tatggataaa tataagtagt tttacttact ttctgatatt ctaaaaactt
    11041 ttcaatgaaa atctcatttt ttggcttcct gtgaaaaata tgtactcctt ccagtcctct
    11101 atctttattt actttattaa tccgataggg ggcgtattat atatatttct atcgagcata
    11161 tactcatcaa aacatagcat tagtttgatt attatatttc tatttatttt aagattatat
    11221 atacaatttg ccccttaata cattgcaatt actaacttct ttagttttga tattaatatt
    11281 taaatcattc ctttttaaag gtgctataag tgctatatag gcattgcaac ctatatactt
    11341 tttaggggtg cagttgcata tgcaagctct ttatttccca acacttaacc agttataaat
    11401 atttatatcc tttattagcc aaatatagca ttttaaacaa ctccgactat ctttagtagt
    11461 aacattttac atagctttat taatgtattt ttgcatctgc aaccatgttt ttaagttgca
    11521 acaccccacc cccacacctt acacaaaaca atacttatct ccgaagccat atacctacct
    11581 cggttcacat atttttcttc aacagcgtta ttttcagcaa ggggggctaa acattatact
    11641 ttttttcagt gcattaaata gccttacatg tattggtata actttatttc aaacccttaa
    11701 ttgaattgtt tcaatctaaa ttaagtaaat tttaataaga taaaatatat agtttgtaac
    11761 tttatttcaa aatacag
//
LOCUS       DAYAOY010000119        11628 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYAOY010000119 DAYAOY010000000
VERSION     DAYAOY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.119"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(162..728)
                     /locus_tag="ACGI6B_003440"
     CDS             complement(162..728)
                     /locus_tag="ACGI6B_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HFG9752853.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            894..1415
                     /locus_tag="ACGI6B_003441"
     CDS             894..1415
                     /locus_tag="ACGI6B_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFG9752854.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2214..4292
                     /locus_tag="ACGI6B_003442"
     CDS             2214..4292
                     /locus_tag="ACGI6B_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFG9752855.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4292..5071
                     /locus_tag="ACGI6B_003443"
     CDS             4292..5071
                     /locus_tag="ACGI6B_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9752856.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            5064..5873
                     /locus_tag="ACGI6B_003444"
     CDS             5064..5873
                     /locus_tag="ACGI6B_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9752857.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5893..6375
                     /locus_tag="ACGI6B_003445"
     CDS             5893..6375
                     /locus_tag="ACGI6B_003445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9752858.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6396..6800
                     /locus_tag="ACGI6B_003446"
     CDS             6396..6800
                     /locus_tag="ACGI6B_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9752859.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(6956..8116)
                     /locus_tag="ACGI6B_003447"
     CDS             complement(6956..8116)
                     /locus_tag="ACGI6B_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9752860.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8324..9874
                     /gene="xylB"
                     /locus_tag="ACGI6B_003448"
     CDS             8324..9874
                     /gene="xylB"
                     /locus_tag="ACGI6B_003448"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFG9752861.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            9970..11307
                     /gene="xylA"
                     /locus_tag="ACGI6B_003449"
     CDS             9970..11307
                     /gene="xylA"
                     /locus_tag="ACGI6B_003449"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFG9752862.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4487 a         1336 c         2073 g         3732 t
ORIGIN      
        1 gcacctcttg atatagattt atagctcaaa tttatatcaa gaggttacgt ttaattaaag
       61 taatacaaat actatctaat taaattatta ataaaaatgt tatataaaaa tgagtatata
      121 gcattttaaa gagtaataaa ccttacataa taaatgattt attaatatga actagaagta
      181 tttttactat attgttttat aaaaccttta ggcattttac ttataaaata taccaccata
      241 atacaagaca aaggtttgtc tactatatta ctaagaatcc ttggaaagaa cgcagcagta
      301 aatatcttct gaccagactg aactaaaaaa ccaaccaata aatcagcacc acttccactt
      361 aaaccaccaa acaataaaac tgaaatagga gtaccaatta aaggacaaac aatagataaa
      421 attactcctg taataatagc ttctttataa ccaaaacctt ttttcttaga aatatatcca
      481 acaataagac caaggacaat atttacaagg gcaaaaggta tatttgtagg acctgatata
      541 acacctaaaa ctaagtttgt acatccacca ataagagcac cccaaaaagg acctaatacg
      601 acagccccaa gtacagtacc aactgtatct aaaaataata atggtatatt tatcatagat
      661 ataaccatac ctaagacaat attaataact atgcacattg aagaaaatgt gactttttta
      721 gtatccaatt ataatacctc ctaaaaaaat atatacattt atcatagtat agattataaa
      781 agattactaa ttgtatattt ctatatagaa tattggaaat atagaacaaa taaataatat
      841 aattatcaaa atatgtagta tcattaaaat taagaataac taaggagatt attatgaaaa
      901 gaaatagaga gattgtagta gtatgtcatt gtatattaaa ttgtaattct aaagtggagg
      961 gattgtcaga atttaaggga gcacaaaata tagcgataaa tttaataaat aaagggtatg
     1021 gaataattca actaccatgc ccagaaatga ttatgtatgg aataaatagg tggggacatg
     1081 taaaagaaca gtttgacaat ttgtttttta gagaacaatg taggcaaatg ttaatacctc
     1141 atgtaaggca gtttgatgaa tatttaaaaa atgattatga tataaaaagt atcattgcaa
     1201 tagatggcag tccaagttgt ggttataata agacatgtag ttctaaacgt tggtttggtg
     1261 agttaagtgg ttgcaatgaa ttgatggata aaataaagga tatacaaatg gtggatggga
     1321 aaggtatttt tatagaagag ctagaaaagc tacttaaaga atataattta gagataccta
     1381 tagttggatt tgacgagctt agagaagata agtagagata taaatattta ttgtctcatt
     1441 atgaaacacc attatatttg gcagaaaaat atgactgatg gacagtacag gaaccatttt
     1501 taagtggagt aattagtact tataaagatg tatccactag ctgaaagaaa atgtagaaag
     1561 gtaataaatg aattaagaag tagataatat atataaagga gtgtctaaaa gtgaactttt
     1621 tagtttatga gacactcttt ttttatatta aatcaagtat attaaatcaa atatatttta
     1681 atcattttag caatgaaaaa taaatttatc tatattttga gacaacatct tttttagttt
     1741 tatttatttg ttgtcgtttt tgacttggta tacaattgta tattttctta aataatttca
     1801 taaattattt ataattataa aatccattgt tttgaacaat atctaatata ttattatctg
     1861 tatttataag gtcactatac atattttcaa gtccgatatt atctacataa taggcaaatg
     1921 ttattcctgt atgtgtttta aaaaaactag atgtatatag gtatcatcac agatattaaa
     1981 agtataaata aaagatatta agaacaactt tccagcaata tattagaaaa ttttactaaa
     2041 atccggaaag cgattgacaa acaaagtcaa ataaagtata ttattggtaa gttagtttaa
     2101 tgtacaaatg aattatatat aaaacctaat ttataaataa cgatactaaa cgacattatt
     2161 taacttaagt tacacaaaga tagaatatta taccattgat ttaggaggta gccgtgataa
     2221 aaatatctga agactatatt gaaaaaagat ttgacaatga actattgcga atagaagcat
     2281 ggggaaaaaa ctctttacgt attaggtcgt ttgtagacca aaattttgtt gatgaaaact
     2341 atgctcttaa cgaaaaacct aagttaaata aagatgatat tacaattaat aaaaatgaag
     2401 atggaagtgc aatcattaaa aatggaaaga taaaagctgt cttagaccat agagatagaa
     2461 ttacttttta taatgataaa aatgaaatat tattgaaaga atatattcga ttaagagctg
     2521 taaaacatga tgatggtgga gaagatgtag ggacaattga gattaccaaa gactttaact
     2581 ctactctaaa gttaaaatca agagaatatc gaccaagcta tagtggagaa tttgaagtaa
     2641 ctactagatt tgaatctgag ccttctgaaa agatttttgg aatgggacaa tatcaacata
     2701 aatttttaga tttaaagaat actgttttgg agttagccca aagaaattct caaatatcag
     2761 ttccttttta tatttctagt ctaggttatg gacttttatg gaataatccg ggaattggaa
     2821 aagtatcttt tgctaaaaat atgacagaat ggaagatgtt ttctacaaat tttatagatt
     2881 attggattac ttgtggagaa agcccaaaag agttaaataa aaattactct caagttactg
     2941 gaactgttcc tatgatgcct gaaaaccttt taggactatg gcaaagtaaa ctaagatata
     3001 gaacttcaga agaagtttta gatgttgtga aggaatattc taaaagagga attaagcttt
     3061 catcaattgc aattgactat tttcattggc ctaagcaagg tgaatataaa tttgacttag
     3121 attattggaa aaatcctaaa gaattagtta aaaaattgaa agaagagtat tcagtagaac
     3181 caatagtatc tgtatggcct acagttcaaa gtgatgcaga aaactacaat gattatttag
     3241 aaaatggcta tttagtcaat gttaatcgtg gtgttagaat gacaatgcaa attcaaggaa
     3301 atacagtctt tgtagatatg acaaatgaaa atgcaagaga atatgtttgg gatagaatag
     3361 ataaaaatta taaacaactt gggattgact attattggtt agatgtagct gaacctggct
     3421 attctgtgta tgattttgat aactatagat ataaaaaagg aaatgtttta agttgtggta
     3481 atatttatcc tattgattat ttaaaaatga tttatgatgg tttacataat gacatagagt
     3541 ctgttgtaac tcttgtaaga ggagcttggg caggagctca aaagtatgga gctttagtat
     3601 ggtctggtga cattgattct agttttgaag cttttaataa tcaagtaaat acagggttaa
     3661 atatgggact ggcaggtata ccatggtgga ctactgatat tggaggtttc catggaggaa
     3721 atcctaaaga cccagaattt agagaactta tggtaagatg gttccaatat gctacttttt
     3781 caccaatttt gagaatgcat ggagatagat tgcctcatag taaaccatta tcaaataaag
     3841 gtggaggctc aatggtaact ggagctccta atgaaatatg gtcttatggt gaagaagttg
     3901 aagtaattct tacaaaattc ataaaaatta gagagagctt aaaaacttat ttaaaaaaac
     3961 tgatgaaaga agcgcatgaa gatggaacac cagttatgag aactttattt tatgaatttc
     4021 ctgaagatga taaaacttgg gaggtggata atacatatat gcttggagat gaaattttag
     4081 tagctccaat aatgaattat aaagatagaa gtagaaaagt atatttacca aaaggacata
     4141 catgggaaaa tatatttagt ggagttagtt atgaaggtgg gaaaacttat gaagttgaat
     4201 gcccactgga agaaattcct attttcctaa agcaagattc cagttataat tttaaagaac
     4261 taaaaaatat tttgggagag gtgaaatcat aatggagcca caattatggc aattattatt
     4321 aatcgtacta tatggatttt ttataaacta tgaaaaaaat tcaacaatgt ttggaacata
     4381 tcaaccagta acagcaggtt ttattactgg tcttatctta ggagatataa atacagggtt
     4441 atatattgga ggtacattac agctattatc attaggtatt agtaactttg gtggagcatc
     4501 aatacctgat tatcaaacag caagtatagt agccacattt ataactatta cgactaagca
     4561 agaagcatct gtaggaatct ctataggtat tccagttgct ttattgatgg tacaattaga
     4621 tgttcttaga aatactattg gtatatggct agttcataaa gcagaagatg gagctaaaaa
     4681 aggaaattat aaaaatataa cctatatgca aatgttagga gtactattaa cagcagctac
     4741 aacaggtatt ccagttgctc tatctgtaat atttggacct tctttaatta ataccatttt
     4801 gaaatataca cctgaatggt taacaggtgg tttaacagta gctggaggac tacttccagc
     4861 agtagggata ggattattac ttagatatct tcctgctaag gagtatttta gttacttagt
     4921 aattggtttt gttttagcag tatatatgaa agtaccatta ttaggtgtag ctttaattgg
     4981 tggagcaatt gcacttataa tttacaaaaa gaatttggaa aatcaagaac aacaatatac
     5041 tgtagtagga ggtatggacg aagatgagtg ataaggtatc attaaataaa tcaaatgaaa
     5101 aggtgaaaaa aagtgtctta tggcgctggt tttttactag ttctgtttct tcaaattatg
     5161 agaagatgca ggccttggca tactgttatg cagtactacc ttttttaaaa gtagtataca
     5221 aagataatcc agaaaagtta caaagtgctg tattaaatca tttacaattt tttaacacta
     5281 atccttgggt tgccccttat attttgggta ttaatgtagc aatggaggag ttatcaagtg
     5341 aagagactga agaggctgtt gcttcaatta aaacaggctt gatgggacca gtagcaggtt
     5401 taggagatag tttatttgtc gttattccgt ggacaatttt tggagctata gcagctaata
     5461 tggcgataga tggaagtcca tttggaatct tactgtggat tgtagtgagt gtagcattga
     5521 aattattaag tataccacta tttaatgctg gttatacatc aggtactaag ctaatatctt
     5581 caattgaaaa aagtttaaag attttaactg agagtacatc tattttaggt ttaatggttg
     5641 taggtgcatt aataccttca gttgtaaaag ctaatgttgc tttgaacttt caacaaggtg
     5701 attttacaat gaaaggtcaa gaaatattag atcaaattat gcctgggtta gttcctgcaa
     5761 ttttagttgt gattgtttat tgggctctta aaaagaatat aaaacctata tacttaattt
     5821 taggagtaat ggttgtttca ataattttat atgcattagg tattttaaaa taaggaaagg
     5881 atgatattag atatggaaat gaaaggtatt aaaaatattc gtatagatga ccgtttaatc
     5941 catggtcaag ttgcaactat gtggagtaat aaattgggtg ttacaagatt aatggtagta
     6001 aatgatgcag tagctaacaa ttctgttcaa aaacaagttt taagaatggc tacacctgca
     6061 ggtatagcgt catctataat tacagaagag actgctataa aaaatattac tgcaggaaag
     6121 tatgaaggac aaaatgtatt attaattgtt aagtctcctg tggaccttat tccatttgta
     6181 gaggcaggat ttaaaataca taatataaat gtaggaaata tgtcaagtag aaaagataca
     6241 acagttctaa gacctaatat cagtgttact gaagaagaga aagaagcatt taaaagactt
     6301 ttagatgaag gaattgaaat tacaactata atgacgcctg atgataagaa aacttatctt
     6361 gtagacatct tatagattta aagaggtgta ttaaattgaa tagtattatt ttgatgagtc
     6421 atggcaatat ggctgaagaa gtcttaaatt ctgcaaaaat gataataggg gattctatag
     6481 attatcctac tgtaaatatg aaacaagatg atggaataga aggaacaata gaaaaattaa
     6541 aaaaagcttt agaagtgtac aaggaaagtt cagaaataat tataatggca gatttactag
     6601 gtggaactcc atgcaatgct gctttattag tagccaataa tgacagtcga attaaaatta
     6661 tttcagggct aaatttaggt atggttattg aatcagcttt tttagataca aaggaattgg
     6721 cagcaaaatt agagaaaata ggtaaagata atatttctat ggttgaacaa aatgcaaatt
     6781 tattatctga tgatgagtag gaagattata ttaatattta aaacgaaata cttatgttaa
     6841 tacttaattg atatacaatt tttaaaaaat atagctaatc ttgcataaac tatataataa
     6901 atctaatttt agaatgtata atttaaaggg ttatggctgt atatagtcat aacccttata
     6961 tttcattaat taaagttaaa ttttgtatat tcaaaaagct cttgatattt acagcagagc
     7021 caccatatag agatgcctca gaccctaatg atgaaatttc tatatttatt cctttgttaa
     7081 aagagctaac taacatatct tttgtaatct gtaatatctc agggatatca ctaattattt
     7141 ggctatttaa atatattatt tcaggagcat aagttgtaat agcattattt atagctattg
     7201 ttaagtaact acaaaactca tgaataactt ttttggcatt atggttatct tcataatata
     7261 gttgctttac tatatcagaa tctattttag gaatattttc taaagaagaa agctgttcaa
     7321 ataccttttt ttctgaacaa tactgttcta aacagccacg atttccacaa ggacatagtt
     7381 taccgttagg catgatgata gtgtgaccaa tttctccact cataccattt ctgccactat
     7441 ataatttatt atttattata atgcctgaac caaatccact gtgaatactg agactaagta
     7501 gtgagttatg tacagttgaa aaagtattct cagctaaagc tgttaagttt gcttcatttt
     7561 ctatatgaat aggaaagtca tactttttgc taaggatgct atataagtca atctcattaa
     7621 gattgtaata tggagtaaac aatactttat tttcacaagt aatcccatgt atagccaaag
     7681 taagaccaat taccttataa ggagtgtcta ttttggatat gttataacta tttataattt
     7741 catctatcaa ttgtataaca ttgtctttac ttacttgtat atctgttaat ttcttagaat
     7801 ttataatagt tccatctaag taagatagag aagaaaatat atagtcgtat ccaatgtcca
     7861 tacttaaaga aatccctgca catttattaa atactaataa tatgggtttt cttccgccac
     7921 tatgagtact atttccaatt cctatttcat gaactagaga ttcatcaata agttttttgg
     7981 taatagcgga tatagttgct ttattcaatc ctatagtaga agctatactt gccctagaaa
     8041 taggaccatt ttttataatt tgttcaagta ccaatctttc attcatttct cgaatagtgt
     8101 atttatcagt aaccaatttg atattcctcc ttaaaataat attgtaatac tttttacaca
     8161 aaaataaaag gttattttgc attgacaaag ataattaaat attttattat tagttcataa
     8221 gttagtttaa tatactaaca aaaataaagc aagtaaaata tacctaaaat ataaaaaaat
     8281 taggatagga aaacgatagt tatgaagtgg cattcaagga gggatgaagt tggagtatgt
     8341 attaggtgtg gatattggta catcaggtac gaaaacagtt ttatttgata aactaggaaa
     8401 tactataaaa agttgtacat atgaatatcc tcttatacaa gagaaatcag gttgggcaga
     8461 gcaggatgca aatgattggt ggaaagctgt agtcgaatct ataagagaag tagttcaaag
     8521 ttcaaatata agcagtgaat gtataaaagg aattggatta tcagggcaga tgcatggtct
     8581 agttatgctg gacaatgaag gtaaaacatt gagaaactct ataatatggt gtgaccaacg
     8641 aacagtaaaa gagtgtgagg agataactga tttagtaggt gaagaaagac tgattgaaat
     8701 aactgctaat ccagcactta caggatttac agcatcaaag atattatggg ttagaaacaa
     8761 tgaaccagat atatataaaa atactaataa aatacttctt ccaaaagatt atataagata
     8821 caaattgaca ggtgaatatg ctacagaggt atcagatgca agtggtatgc agttgctcga
     8881 tataagaaaa agagattgga gcgatgaagt actagaaaag ttaaatatag ataaaaatct
     8941 tttaggtaaa gtttatgaat cacaagagat tacaggttat gtgactagag atgtatcaag
     9001 tttgacaggg cttaaagaag gaacaatagt ggtaggagga gctggagacc aagcagcagg
     9061 agctatagga aatggaatag ttaaagatgg agtagtttca tctactatag gtacatcagg
     9121 tgtagtattt gcttatacaa aagagccaaa aattgataaa gaaggtagag ttcatacttt
     9181 ctgtcatgca ataccaaata catggcatgt tatgggagtt actcaaggag ctggcttgtc
     9241 attaaaatgg tttaaagata atttctgtca aagtgaagtg gaagtttcaa attctttagg
     9301 tgaagatgta tatgagatta taaataatca agtaagtaaa gtaccaacag gatgtaatgg
     9361 attgctttac ttaccatata tgatgggaga aagaactcct catcttgacc cttatgcaag
     9421 aggtgtgttc tttggattat ctccaatcca ttctaaaaaa gaaattgcaa ggtcaataat
     9481 ggaaggtgtt tcatatagtc ttaaagactg tatggatata atagagaatc ttaatataga
     9541 cgttaatgaa gtaagagcct caggtggagg tggaaaaagt aaagtatgga gacagatgca
     9601 agcagatatg tttaatcaag atgtatatac tataaattca agtgaaggac cagcacttgg
     9661 agttgcaatt cttgcattag taggagctgg aatatatgaa aatatacaaa aagcttgtga
     9721 tgctataata aaaacttcaa ctaaattaga accaataagt gaaaatgtag atacatacaa
     9781 tatatatcat agattgtata aaagaatata caaatctttg aaagatgact ttaagttatt
     9841 agatgaagta gttaatgcaa aaataaataa ttaaattagc ttaaaaagaa aaataaagat
     9901 agaaaatagt tttaaattaa gaaaacatac tacatatatt atatgtgtaa aatattagga
     9961 ggacatgaaa tgagtgaaat atttaaagga ataggacaga taaaatttga aggagtaaaa
    10021 tctgacaatg aattagcatt tagatactac aatcctgaac aagtggtagg taataagact
    10081 atgaaagaac acttaagatt tgcaatgagc tattggcata ctttatgtgg agagggtaat
    10141 gacccatttg gagtaggcac agtagagaga ccttggaata atataacaga cccaattgag
    10201 atagcaaaga ttaaagttga tgcaggattt gaatttatgt ctaagatggg aattgagtat
    10261 ttctgttttc atgacagaga tatagcacca gaaggaagag atttagaaga gacaaataag
    10321 atattagatg aaatagttga gtatataaaa gttaatatgg aaaagacagg tataaagtta
    10381 ttatggggaa ctgccaatat gtttggaaat ccaagatttg ttcatggtgc atctacaaca
    10441 tgtaatgcag atgtatatgc atatgcagca gctcaggtta aaaaagctat ggaaataact
    10501 aagtacttag gaggagaaaa ttttgtattc tggggtggaa gagaaggtta tgaaacttta
    10561 cttaacacta atacagaact tgaaatggat aactttgcaa gattcttaca aatggctgta
    10621 gactatgcta aggaaatagg attcactgga caattcttaa tagagcctaa gccaaaagag
    10681 cctactaaac atcaatacga ttttgatact gctactgttt taggattttt aagaaagtat
    10741 aatctggata aatacttcaa aatgaatata gaagcaaacc atgcaacact tgcaggacat
    10801 actttccaac atgaattaaa tatagcaaga ataaacaatg ttttaggtag tatagatgca
    10861 aatcaaggag acctattatt aggttgggat acagaccaat ttccaacaaa tatatatgat
    10921 gcaacacttg ctatgtatga agtgttaaag caaggtggaa tagcaccagg agggtttaat
    10981 tttgactcta aagtaagaag agcatctttt gaggtagaag acttattcct agcttatatt
    11041 gcaggaatgg atacatttgc aaagggatta ttaatagctc ataaattatt agaagatgag
    11101 gtatttgaaa actttactaa agaaagatat gcaagtttta gtgaaggtat aggtaaggat
    11161 atagtagaag gtaaggttgg atttaaagag ttagaatctt atgcactgca aatgccagtt
    11221 ataaagaata agtctggaag acaagaaatg ctagaagcta tacttaacag atatatatat
    11281 gaagtagata caatttctaa taagtaattg tgtagtaatt aaacttggat gtttgaataa
    11341 aataacagga tatgttcttg tttttattct aaaacggaca aagataagta tttgtaaaaa
    11401 ttctcataat catgataaat taaggatata gacttaagta taattagttt atatccttaa
    11461 aatttctata aaaaataatc atttctatag ttatcaatat ttttaattat caatatcttt
    11521 ctcaactaat aaataaaaat cagtaaagta tctaaattta agtcattata atggataaat
    11581 tttttagcat atctttttta gtatatcact gataagaatt tactttca
//
LOCUS       DAYAOY010000120        11240 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYAOY010000120 DAYAOY010000000
VERSION     DAYAOY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.120"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            139..1314
                     /locus_tag="ACGI6B_003450"
     CDS             139..1314
                     /locus_tag="ACGI6B_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9752863.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1487..2884)
                     /gene="asnS"
                     /locus_tag="ACGI6B_003451"
     CDS             complement(1487..2884)
                     /gene="asnS"
                     /locus_tag="ACGI6B_003451"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFG9752864.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(2961..3194)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3363..3557)
                     /locus_tag="ACGI6B_003452"
     CDS             complement(3363..3557)
                     /locus_tag="ACGI6B_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HFG9752865.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3830..5503)
                     /gene="cspC"
                     /locus_tag="ACGI6B_003453"
     CDS             complement(3830..5503)
                     /gene="cspC"
                     /locus_tag="ACGI6B_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HFG9752866.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5513..8911)
                     /gene="cspBA"
                     /locus_tag="ACGI6B_003454"
     CDS             complement(5513..8911)
                     /gene="cspBA"
                     /locus_tag="ACGI6B_003454"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HFG9752867.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9078..10274)
                     /locus_tag="ACGI6B_003455"
     CDS             complement(9078..10274)
                     /locus_tag="ACGI6B_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFG9752868.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10295..11116)
                     /locus_tag="ACGI6B_003456"
     CDS             complement(10295..11116)
                     /locus_tag="ACGI6B_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9752869.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3581 a         2060 c         1304 g         4295 t
ORIGIN      
        1 ataaatttta tctatcacaa ttttagattg ttacacacaa tatattttga tataattaaa
       61 tcataaacga agtattccaa atatctaatg atagaaagga atacttattt ttatgcttta
      121 tttttaatgg aggcttatat gaacttagaa aaaataaaaa aattttttgg atttcctcta
      181 gacaaaaaac acaaagacaa tgttgagaaa gaactctgca attatcttta tcatacgggt
      241 aaaatcattt gcataataat agtaatatct caaataatta tgatgatatc aatctcagct
      301 agagaaggag gcccttttgt cacaccaaga cgccaatggt atttttatat gtatgtaaca
      361 ctattttgtg tatcttctgt ctttatgttt gtattttctt acctttggaa taaaaaaaag
      421 gattgctgta cgttttatat aaatttatgg ataatttatg tgactttttt ctgcctatgg
      481 ggttgtgtaa ttactctact tgaccagtta ggtggaaatg acctaagtgt atttagttat
      541 attatgttag cagctgcagc tttctcagta ttaaaatttg gagaatcagt aatagttttt
      601 ggaggatgct ttttgtttct taatttattc ttacctggga ttcaaactat accaggcaat
      661 gagtttagta atattgtcaa cacatttttt ataacatccc ttgccatttt tgtttctaca
      721 atgctatata gaaatcgtgt atttacttac tatgataaac ttttaattag tcaacaatat
      781 gaagaaatat ctcatataaa taatttactt aacgacaaag ttatgacaga tgaccttact
      841 aaaatgaaca accgtaggta ccttgaagat attctacaaa aaaaattaat cagccatgca
      901 aatcaagaac ctataactgg aatgatgata gatatagatt attttaaaca atttaatgat
      961 aattatggac atcaaaaggg agactattgt ctacaaaata ttgccaaagt aattttagat
     1021 tttacaagtt ctgaaaatga acatgctata cgatatggtg gtgaggaatt ttttatatgt
     1081 atttttaact gtaatgaggc agatgctaaa cttaaatcag aatgtttacg aaaaattata
     1141 gagtcctgca attttgagcg tgatgatgtt cctttaaaat gtgtaactgt tagtattgga
     1201 gtttatacaa agaaaattgg aaagacaata acattaaatg agtttataag taattgtgat
     1261 aaggccctat atactgcaaa agaacaagga cgtaattgtg ttgcatttta ttaaaaggtt
     1321 acatactatt acataaacct ataagtatta tatcgcaaaa taaaagtaat caaattatat
     1381 atttatagta caataaaaat aacttgcata tctctaaaat agataaatat gcaagtcatt
     1441 ttagtgttta tacttaaagt tatataagtt ttattatata taattattaa aattcagcat
     1501 ttttaggagt tcttgggaaa ggtataacgt ctcttatgtt agacatacct gtcatataca
     1561 taattattct ttcaaatcca agaccaaatc cagagtgagt agcagtacca aactttctaa
     1621 gttctaaata ccaccaataa tcttcttctc ttaatcccat ttcattaatt ctatctaaca
     1681 atacatcttc tctttcttct ctttgagaac ctcctacgat ttctccaact ccaggaacta
     1741 gtaaatccat tgctctaaca gttttaccat cttcattcat tctcatatag aaagctttta
     1801 tatcttttgg ataatctgta acaaacactg gacaattata tatttgctcc gttatatatc
     1861 tttcatgctc tgtttgaagg tcacatcccc actcaacagg atactcaaat tcatgcccag
     1921 attcaattaa taattccact gctttagtat acgttattct aataaactca gagtttacta
     1981 tcttatttaa tctttctaac aatcctttat ctataaaact attaaaaaat tccatttctt
     2041 caggagcatt ttctaataca tagcttataa catattttat catatcttct gcaagttcca
     2101 tgttatcttc aaggtctgca aaaactattt ctggctctat catccaaaat tctgaagcat
     2161 gtcttcctgt ataagagttt tcagctctaa atgtaggtcc aaaagtataa acgtttctaa
     2221 aagaaagtgc cattatctcg gcatttaatt gaccactaac tgttaaatta gcctcttttc
     2281 caaagaaatc ttgagtatag tctattttgc catcttcagt ttttggaata tcatttaaat
     2341 ccatagttgt tattctaaac atctctcctg caccttcaca gtcactacct gttatgattg
     2401 gagaatgtgc atatacaaag tttctttctt ggaagaattt gtgtattgca taggcagcta
     2461 ttgatcttac tctaaataca gctgaaaaag tattacttct tggtcttaaa tgtgctatcg
     2521 ttcttaaata ttcaagcgta tgtctcttct tttgtagtgg atacgaacta tctgattccc
     2581 cttctacaac tatgcttttt gcatgtattt cgactggttg tttagcccct tctgtagata
     2641 ctaactctcc ctctacaagt atagatgagc ttattgctaa ttttcctacc tctttaaaat
     2701 tttctacttt ctcatcaaat actatctgca tatttttaaa gaagcttcca tcatttagct
     2761 ctataaatcc aaaattttta gatactctag atgttcttat ccatccagct acttttacag
     2821 tcttccctat gtactgttct ttttctctgt acaattcttt tactgttata aattcctttt
     2881 gcattttaaa tcctcctagt ataattgtta ttaatttctt ttaaatataa tttcaataaa
     2941 aaaagccctt catccctata taaagggacg aaagactatt actctcgcgg taccacccta
     3001 gttgtcataa tgacctctta gttaagcaat gcttaaagcc tataacggtg ccttccgtct
     3061 aagtctactt actaaataag ctttcgatta gaaactccaa gatgttcttc aatataaacc
     3121 tcgtattgag ctttcaccat cctcaactcg ctagaactac attttatatc tactcttctc
     3181 ttcacagtat ttatctttta ttatttctgc taattaacaa aattatacca atttataaat
     3241 ttttagtcaa taaatttata taattgatac tgattgttat ttaaaatata ataattctac
     3301 cttacataat gataataaat acttatcttc attacttctt tattacttca atttccaaaa
     3361 ttttataatt tacttccatt gtcaacccag tcttttgctt cttcgacacc cttaattgaa
     3421 ggtattgaaa ctttactatg aggttttaat ttttcaacat cagcccatgc tgctgtcgac
     3481 tcatttccaa ctaaaagtga acttttaagt cttttattat ctttaccttt taatgctctt
     3541 gcctttttac tttccaaaac tatcacctct attatttatt ttgtaatagt tttagcaatt
     3601 ttacaaataa aattcataag tctaaattat aattattttt aaattaacat aatatctttt
     3661 caatacttaa tttataccta tttatataca aaatatttac tttgtgtatt caaaatataa
     3721 aactgttttt taattttcat atttattttt tatgctattt ttctactcta tctaaagtat
     3781 ttgatacaat taaactaata atcaattata attttacata ggttcttatc tatagagtat
     3841 ttgctatctg ttgaatcgta ttttttaaat ttaaaatccc atatccctgc gatacatttg
     3901 gatatgtata tatctcaagt tttgtagcac ccaatattaa atatgttttt aaaacttgtg
     3961 taaacaatga taatctcggt acattatcct gtttttctaa gtattccatc aataaggcta
     4021 aaacacctgt gactatagaa ctgctcactc ctgttcctgt tcctgtatta tatgttccat
     4081 ttttatatgt ggatattata tctacacctg aagctactat atctggctta attcctcttc
     4141 ctctaattgg tcctttcgaa gaaccaatcc acatactatc agtcttattg ttaaatgtac
     4201 caactgtaat tacatcatca ctagcagcat acatagttat agtagctact gaatcaggct
     4261 ctaaaaacct tgtgtctgga gctatcaaat ttttatttgg caagtatatg tcatattctc
     4321 catttattat aagttctgaa gtcaatctta aaatccatac tccaggtttt atatctctta
     4381 atcttatctc caaattttct tttccagagg taatatatgg ataaataaat ctcattgcat
     4441 aagtagtatt ttctaagtta aattttcctc tatatatata aaagtctgga gaatacctta
     4501 tatcatgact aacttcacct gaaggagata ttatctgtgc tcccacttta tctggaccat
     4561 tcgtatttag tgttatatca agagcatagt catctccatc ttgtattatt acatcttgaa
     4621 cttctcccac actgctaaat ctgccagaat aatgaatatc tgtattccct tgatttcctg
     4681 caccactcac tactaccaca ccagcactac tcaatatatt aaaagtatct agtattgaag
     4741 tagttgctac agcacttgac ttaactccaa tagtcaaatt tattatcagt ggtttatttt
     4801 ctgttcttgc tatgttagta acatacgtta tagctgctaa aaaatctgaa acactgtaat
     4861 ttatccttcc agcataataa gtatctgtat atgattttaa tttaactact attaagtctg
     4921 attctgtagt aatacctctg tactgagaat ttatccttcc atttccaact aatataccag
     4981 aaactaaagt tccagtacct atattatctt gactcaaact cccatcattt ctatttatgg
     5041 ctatatttaa ctcacttctt gtaaattcac ttccaaaaat aaaaccttct ggaggtggat
     5101 ttgtatttgc ttcttggtcc cataaatata aaactttact agttccatca tcatttataa
     5161 aatcaggatg caaataatct atcccagaat ctataactgc taataaaatt cctctccctg
     5221 ttatatcatt atatggattt tcataaatat aatcagtttc agctgctgtt gtaatggttt
     5281 ctccattatt tacattatta gttatttcaa ttagactgct cattggttca gattcttccc
     5341 accaagcaac ttgtattata ttatttaaaa tagtttcatc aaaatctaaa ggtacatata
     5401 ttactgcaag ctgactattt aaaaccatat acctatttat tccattttct tgtagggcag
     5461 attctatatc accttgataa attatacaat aagatttttc cataaatccc tcctatctta
     5521 aaacatcaaa cattcctcta acatccaaga gcccatatcc agaatttgta tttggatata
     5581 ctgtgtttga atcttttcta gcaccagctt gcataaaagt ttttattttt tgcacatatg
     5641 cttggtttgg atatctaccg tcaacaaaag tatattgaaa atacattgca gcagcaccag
     5701 ctgcatgtgc acttgctgct gctgtaccag ttatagttgc atacgtgttc ccaggataag
     5761 cagctattat attgacacct ggtgctacta tatctggttt taacctgtct tcaatagttg
     5821 gccctcttga tgaactttgc cataaactac cattaatggt attgtaggct cctatggtta
     5881 ttaaatcatc ttgaatcgca ggataattta tagtgtaaaa agggtctacc tctctaaatc
     5941 tagttccact ttttaaaagt tctctatttg gaaggtataa gttatatctt cctgttataa
     6001 tataaactcc tactaatcga atcttccata cacctctttt tgcattcttt aaagtaacat
     6061 tagtaaattg ctgtccagaa aaagttgttg ggtatatata tgttatagaa tattcagtcc
     6121 cctctaaatc aaataaacct gttactttat tataatttga aattccaaca cttttacttt
     6181 cttcaccagt gggagatact attattacat cagctttatc tggtctattt aaccaaagtt
     6241 ctaatgataa ttcttcctcg tcttcattta actctaattc aacttcaaca gaacctccaa
     6301 catatggtat tattccagat gtatgtgtct gtgtattgcc ctcatttcct gctcctgccg
     6361 ttatacataa ccctctagta aagaacgctt tttcactatt actcctattg gttaaacctg
     6421 ctaggctact ggtacctaaa gacatattta taactaatgg tcttccaagt tcaaaagctt
     6481 ttttatatgc atactgagat gcagcaaaca acattgcact attataaaaa tcatctatct
     6541 tgccgagttt tattattatt agctcagaat cttctgctat acctgcatat tcactattta
     6601 catttccaag ccctgcacat atcccactca acatagttcc ttgaccaact tcatcctgag
     6661 atagactagg gtcattttct gcgatagctc gatttatatc ttctcgtgta tattctgtcc
     6721 caatataaaa accatcagga ggagttcctt cttttgtttg gtcccataaa tatactattt
     6781 ttgaagtgcc atctggataa ataaaatctg ggtgtagata gtctattcct gtatctgcaa
     6841 ttgatattag agtccctctt cctgttatat taatattagg attattttta aagaaattta
     6901 cgcccatttc ttctgttgca actacacctc caaaagtacc tcgattaatt tctccaagta
     6961 atgtcatctt tgtagtcgat acagttctta tgattgaagg aagttgcaaa actcgttgta
     7021 tgctattata gtctgtatta tttatagata gtacacctaa tgaatcactt atcttaaaaa
     7081 acctttgact catacctaac ccagctagtt cttcttcaaa ttcaggagta tggactatat
     7141 ctattcctga tagtattcct gattgtctac ttgcattttt acttatataa atttcattct
     7201 ctaagtcatt atgtatttgc atattataac catctataaa cttataacta ttttcctcat
     7261 ctttaaatga cttattaata cttctatatc caatatcctc tgtttctaaa tcttgattta
     7321 tattagatag agtgtacaaa tctatatctg ataaatttaa aaaaccaaat cctgatgaat
     7381 tatttggata agattgatta cttaatctcc ttgctccctt tagaagtaat gcccttaatt
     7441 tctgtgaata caaaaataaa tcatttccat ttacaattcc ccattccata aacaatgaac
     7501 aaacccctgt aacatgaggt gtagccatgc tagttccagt taatgctcca cttgtacctc
     7561 ctggtaaaaa agatactata tcttcacctg gagctaataa atcaggttta aaaacaccta
     7621 attgtgtatc tccttcacca gaaaatatag atactatatc tgttcttgaa ttaaaactac
     7681 ctactgttat aactctactt gctgttccag gaactgttac agtaagctct tgagtaggaa
     7741 tcagaaatct agtatttcta tttagacctt cagaagttgg caaatatata tttacattac
     7801 cagttactat atctattggt tcaaatacaa ttttccataa cccaggagtt atctgtgtat
     7861 ttgaacttaa ttgtagagta actcttcttg tgagagaata cggtgctata gggtaaaaat
     7921 aaccagttat tctagtttca cccaaagtat tccttatctc acctgaagtt agagagattg
     7981 cttgtgtttg attatttgat gggtttacca aatgtacact aaaatcatca acaaaatctg
     8041 gccatatatt tatgtttaat atacgctctc cttccccaac tataaactca acttcttctg
     8101 taatattatt ttgaagccta atccttttat gacctccttt atctgcatta tttccagctg
     8161 ctacaactat attgtttttc caaaataaac acatatcatc tatatattgt tcaaataaag
     8221 atgttcctct atgtgaacct tcattacttc cataacttat atttaatgta acaggcattc
     8281 ttaattctaa tgccctatct aaaataaatt taattgccct cataaactct gtacttctcg
     8341 aaaaaatatc ggtctgtata tttccaactc taaccactat tattctggca tcacttgcaa
     8401 tagttgcaca aatacctgca acatgtgttc catgtaagct agttgttgaa ataggtatat
     8461 acatacttcc atctattgca ttatttatat cttcattagt atataatgtt ccttctctaa
     8521 aaccttctgg tggatttcct tgaatagatt ggtcccaata atataatatc ttagaccttc
     8581 catcactatc tctaaacaca ggtagagtat aatctattcc cgaatcaatt ataccaatta
     8641 tagttccttt tccagttaaa ccagttctat ttttaaatcc tgttattcct gtacttgaaa
     8701 aactttgcac atcctgagtc tgtaatataa aaggcttttc tataaattca atttctggat
     8761 aagttaaaag tatatttaca tcttcttcat tacttgatgt tattattgca tatgatgaat
     8821 taagtatctc tactgataca cctaattctt cttctaacct caatatatct ccattgtatt
     8881 ttacaattaa ttcataattt attataatca cctcgacttc taaaattatt attaatgtat
     8941 atgttttgtg tgagtctaaa atgtattaga ctatataatt ttttaagttt cagttataca
     9001 atttataata gatatattaa aaaaataaat aatgatggta caattttgaa taaatcatac
     9061 catcatttat atttacttta tcttgaaacc attaaatttt tcaatgcttt ctcaagacct
     9121 tttacagtca atggaaacat attaaattca ctttgtatca ttctttgagt ttgataccaa
     9181 taactaattg aatcccatcc atctctaagt tctggattca tccatacaat tttactgtac
     9241 tttctcttaa accttttaag ccactctata ccaggtgtat aattatatgg accacgatag
     9301 ttaccaccaa tatgaagcaa ctcagaaggc gacatagaag catctccaac aattataact
     9361 ttatagtttt tatctatatt ttttaatatc cattcagtat taatagattt acttaaccaa
     9421 cattcaggtg ttgtaaataa tctatcataa atacagttat ggaaataata tatctttaca
     9481 tctttaaaat gatttgattt acttacagac tgaaataatg tattacataa agtgctatac
     9541 cctctcattg aaccacctga atccataagc aataaaagct tcactgtatt ttttcttggc
     9601 ttctcaaaaa ataactttaa atatcctgca ttattacaag tctcttcaat agtcttatcc
     9661 aaatcaagct ctgtctttgg agcatctatt ctagtagaaa attgacgaag tctctttagt
     9721 gcaacttgaa attgacgcat atttaaaact tcatcatctc taaaatctct gtattttctt
     9781 tctcctgcaa cttcaagaac agctctacca taagtagaat ttccacctat acgtatacca
     9841 gtttgccctt ttcctgaatg tcctaactct gaagtgcctc ctgtgcctat ccaataatta
     9901 ccaccattat gaggagaatc ttgttctttt attcgttcct ccaatttagc tcttaattta
     9961 tcaagattta tatttggatt ttgttctaat ctttcaaact cttctaaatc tatatctggt
    10021 tttttaagcc aattcataat ctcttctgga atttcttctt ggtgttctat acctttaaaa
    10081 tactccaaaa acactgcatc aaacttatca aaatcacttt cactttttat taaaatcatc
    10141 ctgcaaagat agtaaaaatt tgaaaagttt gaatagcaca gtcctttatc caatgcttca
    10201 attaaagtca tccattcatt taaagataca tttaatcctc ttgacctcaa taaataaaaa
    10261 aattcaataa acataattat ccagctccta ttaactataa gtgtctttgc atagattcaa
    10321 tatcctcatt attcttaagt agtattcctg caaaaggaac ttctttttct attttttcaa
    10381 taggcattcc actcaaagtt aatgcttgta tccagtctat taactctgat gtactaggct
    10441 ttttttgtat gtcctttaaa cttctaatcc aataaaaagt agtcattact tgttccaata
    10501 ggtgttcttc tactttatca aaatgtactt taactatttc ttccatcatg tctctatcag
    10561 gaaattctat ataatgaaat atgcatcttc ttaaaaaagc atctggaagt tctttttccg
    10621 catttgaagt tataataact attggtcttt gtttagctct tacagtttct tttgtctcat
    10681 ttatgtaaaa ctccatttta tccaattccc aaagcaagtc atttggaaat tctaaatccg
    10741 ccttatctat ttcatctatt aaaagaatta cttgttgatt tgagctaaag gcctctccta
    10801 gtttaccata ttttatgtac ttgctaatat catctacacc ttctcctcca aattggctat
    10861 cataaagtct ttgaacagta tcatatacat aaagcccttc ttgtgctttt gtagttgatt
    10921 ttatattcca aattactaaa tcttttttta attcatttga aattgcttgt gctagcatag
    10981 ttttacctgt tccaggttcg cccttaatca aaagtggctt ttcaagagca atagcaacat
    11041 taactgatgc catcaattct ggtgatacta cataatcact acttcctcta aagttagata
    11101 tctctttttt tatcataaat cctactcctt ttaaacagta ttttctaaat gtatatagac
    11161 tttattacta tttttgatat aaaaataata atacaataca tttgtatagt attattctaa
    11221 aattctatat ctaaataaaa
//
LOCUS       DAYAOY010000121        11084 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYAOY010000121 DAYAOY010000000
VERSION     DAYAOY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.121"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(39..671)
                     /locus_tag="ACGI6B_003457"
     CDS             complement(39..671)
                     /locus_tag="ACGI6B_003457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9752870.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(764..1096)
                     /locus_tag="ACGI6B_003458"
                     /pseudo
     CDS             complement(764..1096)
                     /locus_tag="ACGI6B_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1174..1377)
                     /locus_tag="ACGI6B_003459"
     CDS             complement(1174..1377)
                     /locus_tag="ACGI6B_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9752871.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1767..1997)
                     /locus_tag="ACGI6B_003460"
     CDS             complement(1767..1997)
                     /locus_tag="ACGI6B_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752872.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1999..2352)
                     /locus_tag="ACGI6B_003461"
     CDS             complement(1999..2352)
                     /locus_tag="ACGI6B_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9752873.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            complement(2766..3215)
                     /locus_tag="ACGI6B_003462"
                     /pseudo
     CDS             complement(2766..3215)
                     /locus_tag="ACGI6B_003462"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3229..4155)
                     /locus_tag="ACGI6B_003463"
     CDS             complement(3229..4155)
                     /locus_tag="ACGI6B_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752874.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4280..5062)
                     /locus_tag="ACGI6B_003464"
     CDS             complement(4280..5062)
                     /locus_tag="ACGI6B_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9752875.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5218..5838)
                     /locus_tag="ACGI6B_003465"
     CDS             complement(5218..5838)
                     /locus_tag="ACGI6B_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9752876.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5965..6633)
                     /locus_tag="ACGI6B_003466"
                     /pseudo
     CDS             complement(5965..6633)
                     /locus_tag="ACGI6B_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6704..7540
                     /locus_tag="ACGI6B_003467"
                     /pseudo
     CDS             6704..7540
                     /locus_tag="ACGI6B_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8212..8979
                     /gene="aroD"
                     /locus_tag="ACGI6B_003468"
     CDS             8212..8979
                     /gene="aroD"
                     /locus_tag="ACGI6B_003468"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9752877.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9307..9984)
                     /locus_tag="ACGI6B_003469"
     CDS             complement(9307..9984)
                     /locus_tag="ACGI6B_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752878.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10134..10451)
                     /locus_tag="ACGI6B_003470"
     CDS             complement(10134..10451)
                     /locus_tag="ACGI6B_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752879.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10473..10811)
                     /locus_tag="ACGI6B_003471"
     CDS             complement(10473..10811)
                     /locus_tag="ACGI6B_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752880.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3684 a         1744 c         1571 g         4085 t
ORIGIN      
        1 tattaaagta aagtttaata taaataaatt ataaaatatt aaactaattt atcaatattt
       61 cctgattgaa tatgcttaat gtttgattga atttttttat atggccaatc ccaccattta
      121 atttctaata attttgaaat tgtatcatta ttaaatctct tttttataat ttttgcaggt
      181 actcctccaa caatagaata cggtggaacg tcattagtaa ctactgctct tgttccaata
      241 attgcaccat caccaatatt tacacctgac ataattatag aatcataacc tatccacaca
      301 tcatttccaa ttataatgtt acccttattg tcccaagcgt ccgttatatg gttgatatcg
      361 agtttccatt cttcataaaa gatgggaaaa gtatatgtgg attttgattt cattgagtga
      421 tttccactag tcattagaaa ttttgcatta catgctattg agcaaaactt accaattatt
      481 aatttatcat tgtttatagg gtaatgatac aatacattgt ttttctcaaa atctcttggg
      541 tcattataaa agtcattata tattgtgtaa tctccaactt ctatattttc ccttgttatt
      601 acatttttaa gatatacagt ttgataatca ttacttcttg gatatacctt atttgaactt
      661 ggaatagtca tatttaaatc tctcctttaa tatatattat gtttttgact attccactac
      721 tacaatacat ctcttcacag aatcactcct tgttaagaat cttcataaga taatggcgtc
      781 cttttttagt gttaagtcgg atattctttc ttagtccatt ctttttagcg tataactgac
      841 aaactgtcat tttcttacca atagtgtcaa tgctatcatc aaggtcattt tgtattgctt
      901 tttccttatc ccttaatgat atatcatcac gttttccctt tgggggttta tcagtaggta
      961 caagtttcca agagtaaaca aattgtggtt taccaaatga atatacatac ttatatgcgt
     1021 atcttccatc ttttctctgg ctctctccag ttcttaaaat tctatcttta ttatctcgtc
     1081 ttttttcagt tgacatgata agaagctcct ctcaataacc gaattgagct tgatacgtca
     1141 tatgaatatt ataacatata aggctcaatt tcattagata acgtcaagtg tatcaacgat
     1201 tttctcaaat tgttttctct ttatttgaat gcgattgcta ttcataatga cccagccaga
     1261 atcaaggttt tcatcagcac gtttacgtaa ttttttctct ccaatacgaa agtattttga
     1321 cgcttcttca atcgtcaagg tatatttttc ccaaataggt acatcagtat tgttcatata
     1381 atgtatcact cctttctatg ttcgtagttc agaagtagat agatggcttt gataagctcc
     1441 attggtattt caaccaatcc cattcttatg gacagaaccg tgtcatacag gtgtatcatt
     1501 atactgtggt acaggttacg gcaaaacaac cgttccaaag gttattcaca aatcactata
     1561 tagtagctct cttgattaag gtcatggcgt atagtatttg ttggctatct gtatcacaaa
     1621 cgtatctatc tactttattc agttgtcaaa gaacagtcgg cattgatttg cctataaaaa
     1681 atcacaatag cttgtgggct tttatagaca aataattagt gattctccat gtaccaaata
     1741 gcttgataca tggagagagg gcgagattaa gacaaattaa atctcaaaat agcagtgatt
     1801 agttttgttc ctagcattcg ctttgtttct tcttctacat gaatataggg atttccgtat
     1861 tcatcaaata aagttttctg tgaaagacga atgatatagt gttcaaaatg tttaagtatt
     1921 acgtttattg catttacatc gccatgtaca gcaagtaaaa taattgtaaa aggtaatact
     1981 tctttagtag gtttcatttc aatgttcctg catgagttct ttcaactctt ttagtgcatt
     2041 tgttttgtga taatgtatgg tattttctgc taaatccatc aatgttgcta tgtcagcatt
     2101 tgtcatgttg aaaaagaatg aaagtagaat tatatcttgt tttctttttg taagttttcc
     2161 gattgcttgc gccattaata catcttcaat tcgtatgttg tagtcgtaag aaaccataca
     2221 gatgtagtta gaatcataat catctaaaat actcaattta cttaattctg caagtgacaa
     2281 agattcgaga gataccaaat aatcatttct gcgtttattt tcgtcataaa tatctctagc
     2341 atagttacgc aaaactgttt tacagaaact gtcgaattga tgttcaattg tcttttcatc
     2401 aaccataaga tttcaccctc tttccatcgc aaggagttaa ataaccttgc ttactcctat
     2461 cacggtggga gggcgttaaa tcttcgataa atttcaaaaa aaagagaaaa aaatacacac
     2521 tctgcctata gggcaaaaat gtgtattctt taaaacgtat ttaatttata ttggttgtaa
     2581 gccaccacca tattgatgta tggaaatatg cattggctag cttttcataa aaatacaaag
     2641 atgcataaga aattaataga ggggaaaaac ttattctgtt attaaaaagt aggttaattc
     2701 taataaatgc tattgcggtc gaattaaact atatacatgg caatcaatca tgtttctatt
     2761 ctttattatg aattgtgaca gtattcttca atcttaaagc cacatttttc caatacttta
     2821 cttgactctc tattgtagga atatacatta gcatgaattt tgttaaggtt gtatttttca
     2881 aaggcaattt tgcatatttc ttttactgca tttgttgcta tccctttgcc tcaatagttc
     2941 ctagcaagcc agtaacctaa ttcagcattt ttattaatag aggtatcttc ttcaaatgtt
     3001 aggctgatac ttccaatagc cctttcttga taaaaaatag catagctaag ttgtttaaca
     3061 tctgaagttg ccaaacaaaa actaacaaaa tctctagcgt tttgctccct ataagggtgg
     3121 gggaagttgt cacgaagatt ctttgcaatt tctttatcat tcgcaaactc aactaaatcg
     3181 gtaatataag cataattcca ctctataagt tccatttttc ttctcctttt atgaaacagc
     3241 tacaagaaat ctttctaagc caataccact tatcaaacag cgttctcttt tatttccaag
     3301 catttgataa gtccagtcaa caaatccacc gtcaactatt tcaacggttt catttccttg
     3361 tttgaccgat aatttgaaat tgataccttt ataataatta ttatcaacat catcattatg
     3421 aattgataac tctatattag gaaaaataag tttgatagtt tcaaccattc tgtcgaaaaa
     3481 tccgtcatta tctttgtatc cgtttctttt gcgtagaata atagagaaac gaacattttc
     3541 tatttcactt aatatatctt tataatagtt taagtgcttt tctaacaaca ttttttcaca
     3601 agtgtaagac cctgtatcta taccagatga aaccatacaa tataagccaa aatgtgaata
     3661 taaaccttta cctacaaaat tttgtgctct tattacccta gcagtagtag ccatgtggat
     3721 aggtatggta ttattttctt cttttctttt taatttatca gcaataatta ttgccaacat
     3781 atttgagggg tctgcaagtg tttctgtccc tcgtagagca cttactacgt tatattgatc
     3841 tacggagcca aaaacgctac agctacctaa tggggcagag ggagaaagca taatcgtctt
     3901 gatatccata ttgaatgctt ttctcaataa ttttgattcc aaagcatgaa tttcttctgg
     3961 atctataccg ctagggatag taaatctatt tagttggtat gactttagaa tatcagttgc
     4021 agttaaagta tttgcttgtc tatcaaacac ttctaataga agtgagttca aatctgattt
     4081 tgatagagct gataacttat caactatatt cttatcccca attttatttg taattctact
     4141 taaaatatca ctcatagtta tcctccatac tgttactttc aaaataatta tagaattgtt
     4201 acaacaacta atctaggtca tttgaccaaa aatgaaaaaa cactgaatat ttactattca
     4261 atattttttt actttgggtt tatttgatta aatcttgaaa atactgtaat gcaacccttt
     4321 cagcttcaac ctttcggaca ataggacttt gtgataattc ctctcttaac tcaagataca
     4381 ggttgatagt agcctgcaat gcattaacac agcttttata ttcatggtta gcaaccgtct
     4441 tttctaatgc ttcaatatat tggggtgcgt ccaattcaat ttttctaact ccacgaggta
     4501 atttacctaa gagaagctgt attaatgggg caagtactga ttgtcttaaa aaagataaaa
     4561 aatcaattgt ttcaaatagt tctctacgtc caatcttagt gcaagcgtaa tgaacccaaa
     4621 tccaaaatct atcttcaatc cactgtaagt ctggagttgg gaagtatgct tcttttttag
     4681 aataaatttc tgttatgcag ttgttttctt catagagaat agctgaatct tcaaccctat
     4741 ctagtaaacc atctaaggtt gtaaacttaa aatcaacatg aagaacaggg gaatcataga
     4801 gggaaattat caatcgaggt tctccaacgt gttcgccagt aaaagcagaa actaatgttc
     4861 caaactgttc cacaataagt aatctctctt cgataacttc ttcataggat ttatcatctg
     4921 ctacaatcag aaaatccaaa tcagaatatt catctaacga ttttgtgata taagaactgc
     4981 taattgcaag tcctattatc ctactatctc tttttgaaaa ttctaatacg ttttgtatca
     5041 ttttctgttg tgttttagtc ataatataac acctctcact gtcttttatt agctatcaat
     5101 ccaacataat tatgcatagg agtattatat aatgaagaaa taaaaaaaaa taggtcactt
     5161 gacccgaaat aaaaaagcat tgaataatta ttattcaata cttttttgaa attatactta
     5221 tgttgtagga agattcttta atacctctcg ctgattaatt acactaaatc ttttgttttc
     5281 ataagcaatt agatgttctt gtacacattc atttatagca cgattaatgc ttctaattgg
     5341 agcgtttact tcgctagcaa gctgtttttt ggtaagcaga tttaattcac ttctataata
     5401 gtgtaaggat atacacctca gaagtcgttc tttaacactt aaagatgata attcttcaat
     5461 taattcagca tttacaacta atttactaga tatttctcga atcagaaatt ttgtagcttc
     5521 aaaatctccc cttaaccagt ttaaaaaatc atctctatga agtttatata cattacaatc
     5581 tgtgaaagcg acaatttcaa cgggtttttt cccatcatta aattgttcca tttctccaaa
     5641 aaaactacca cttctataaa gatgaatcgt tgagatgttt cctttcacat ttaagatgaa
     5701 tgcttctgca ataccgtcaa tcataagaat tacatattca ttttcttgct cagcaaaaag
     5761 aatagtctct ttctttttat acgttttttt aacaagcttc tttttaatat cttcattcat
     5821 aaaatcaatt aaatgcatat tcatccctcc ttaccaaatc tttgattaaa ttatatcatc
     5881 tttcaattag ttaggtaaga tagtaaaaat atatttacat agaaactatt ccattattat
     5941 tttccagttg tcatttttct gcaaaaactt atcactgtca cgactggaga taccagtaag
     6001 gaatgttagg atatcaatcg ctaaactttc agaatccttt ttacggctca aaatgacgta
     6061 agggtcaagc aatcctttgt tttcatcatt actggttaaa ttgacaatgt ttatctcatg
     6121 ggctatttct ggtagcgttt ctttccattt gccgcgttct gctttagggt caactatgac
     6181 cgcctgtccg ccgaatagta ccgcatagta aacaagcata ttgttactga ataatttacc
     6241 accacccaat gaaccgataa acgcagacgc taacgcattg gtaatagaac cctttacccc
     6301 ttgactagca agggcaggtt ttagatagac attctttcca gtgtccagat tgtagccaag
     6361 atagatatcc tcatgttcgc caagggtttg tgtcgctcca aaaccaagtc cagccaaaaa
     6421 atccgaagtg acatattgaa tataatcatt gatgtaacgt ttgctgacag gaataaattc
     6481 gccatgcaat cccaacatat ccccaaaggg acgcacgagc ttgattgata agtcgtcgta
     6541 aaagtctttg acttcatcac aacgcctttt tagctcgtca agattctttg tagatacacg
     6601 gacaacatag ctaagtttat acatagattc tttacagtta aaagtctgtt tttaaaaaaa
     6661 tgtataaatt ataaatcaaa atatagatac tatcaatgac ccactaggtg caaaaagcat
     6721 tgatatggtt aaattatcag acgctaaaag ctgggctatc cgaatgagtg aagatgggtg
     6781 tgcttataaa tccattgaca attataagcg ctcactgaaa gcgtcgtttt tcatggcaat
     6841 tcaagacgac tgtattagaa agaatccatt taatttttag ttaagtgatg ttttggaaga
     6901 tacaacagaa ccaaaaatcg ctctaacacc agaacaggaa atgggcttat tatcctttat
     6961 ggggagcgat aagacttatt gtaaatatta tgatgatgtt gttatcctct tagaaacagg
     7021 actacgcatt tctgaattat gcggtttgac tttagaattg gatatgaaaa ataaagccat
     7081 tatggtagac catcaattgt tgagtgatac taagattggc tattatgtag aagttccaaa
     7141 gaccacacag ggtaagcgag aaattccaat gactgaaaga gcttatcaat catttgaacg
     7201 tatcgtggaa cggcgtaaga acgctaaagc agaaccaatc attgttgatg gttaccagaa
     7261 cttcctattt cttaaacaag atggattacc aaaacaagca actcattatc aaagcatgtt
     7321 aagagcgtta gtcaagaagt ataataagac ccatgaggac aagctaccaa agattacgcc
     7381 acataccttt aggcatacac tctgtacaaa tatggctaac aaaggaatga gtcctaatat
     7441 attgcaatat attatggggc ataaaaacat tactatgacg cttggctatt atgcacatgg
     7501 ttcatttagt tcttcgagag ctgaaatgga acggttataa tcctagtagt atgactagag
     7561 tttactactt atttactact tttgatggca ttcttatacg gttaaatgcc aacttatgca
     7621 aagtatcttc caaaaagaaa atgccgatac tccttgtaga tacaggatat atcgacattt
     7681 accaacttat gaaaagataa tcaaatttaa tataagtttt tcaaataaaa agataccaaa
     7741 ttctttttcg agaatctata taacattact taagcttggt tggggtataa acaattgagt
     7801 agctactttt gtatggtgtt caactttagt caaaactctt aagtaatgca aatgatttag
     7861 attcattaat attcaatctc ttcataagta gtgaaattcg agttatttct gcataattat
     7921 actatgattt atagattaat tctattagca tccagaataa tattgtttat atgagtaaaa
     7981 tttaaagaat attgtgaaaa ttataggatg ttaaaataaa tttaggtaaa aaagaagtgt
     8041 ttgtatatta tagagtacct tttctaaaat atgtgggatt gttttaagaa ttagaataaa
     8101 aattattttt aaattagtat ttttataatg tatagtattt ttaatgtata attaaattgt
     8161 gcagaaaata gaatttaaag tcctatagaa tttctgatta tggaggagaa tatgaaaaga
     8221 aaagttcaag taaaaaatat tactattgga gaaggtagac ctaaaatttg tgtaccaatt
     8281 attgggaaaa ataaaaaaga tattataaaa gaggctaaag agttaaaaga tgcatgtttg
     8341 gatattatag agtggcgagt tgattttttt gagaatgtag aaaatattaa agaagttaag
     8401 gaagttctct atgaattaag aagttatata catgatatcc ctttattatt cacatttaga
     8461 agtgtagtag aagggggaga aaaattaata tcaagagatt attatactac tttgaataaa
     8521 gaaatttcta atacaggttt agtagattta atagatgtag agttatttat gggagatgag
     8581 gtcatagatg aagtagttaa ttttgctcat aaaaaagaag tgaaagtaat tatatctaat
     8641 catgatttta ataaaactcc aaaaaaagaa gagattgtat ctcgtttatg tagaatgcag
     8701 gaattgggcg cagatttacc caaaatagct gttatgccgc aaaatgaaaa agatgtgtta
     8761 gtgctacttg aagctacaaa tgaaatgttt aaaatttatg cagatagacc aataataaca
     8821 atgtcaatgt caggaatggg tgttataagt agattgtgtg gtgaaatatt tggttctgca
     8881 ttgacttttg gagctgcaaa aagtgtttca gcaccagggc aaatatcatt taaagagtta
     8941 aattcagtac ttaatctatt acataagagt attaattaaa ttaattatat agaaatatta
     9001 gacggtattt ttgaagatat aaattaaata taaaaaactt ttcagatagt aaaatttaaa
     9061 ttgggcaatt atatctgaaa agcaactatt tagtgtgtat tttgaaagtg atatagaaaa
     9121 tcttataaat caactatggc atatttaaaa tatgccatta aaacctacta gacattagat
     9181 tatagatggt atactagcat ttctattatt tatagatagt aagttttcat aataactgtt
     9241 ttatctaaag tagaagatat atttgtatta tgtatatttg tcattatttt tttgtaatta
     9301 tgtatattaa tggttatata tgtaatataa tattatcact ctattttttg tatattgtac
     9361 attacaacat ttatatctat catcatcaat tacagtagaa tgtatgggat acttattttc
     9421 acttaaccaa tatttttgca tattctttgt ccaagacttt ggaaacttca atactttctt
     9481 gtttattata acatcctcat cttcaaaaat attaacttct ctttctattt ttatttttct
     9541 gttgtatatc ttattgatag attctttgtc gagttttatg tctctataca tatcagggca
     9601 atagcaacaa acataatttc ttttttttcg acgtttaaat ttcacaactt tctctatgct
     9661 aatttctaat ttttttgtac catcttttat aaatttaata tcttctatgt tctcgcttga
     9721 aaaaatttct gtcaatctta aagtatctac agtttctcta attatttgct ttttataaga
     9781 agagaatcca tatccaaaag aaaggtttaa tttattaggg atgtagccat ttttatattt
     9841 aaacatctta attttttcca attcagcatt atctttaaat ttcaactttt catacctaaa
     9901 agttactttg ctaaaaagag tatattctac atcaatatat tcttttgcaa tcttaaattt
     9961 atcgctacct tcaatcacaa tcatagccta tctccaatcg ttttatatta aaataaggcc
    10021 atagaacatg gtctaatagt actcattagc actataagac aatgttaaca caaaaagaca
    10081 aaaaaaggtt atttatttca aataaccttt ttttttatat aaaatataaa gaattatatt
    10141 ttattctttt ttatgatgtc tataatctgt gatatcagat ttagttctct tgaatttaag
    10201 tcttttaact cttcatttat atcattttct aaatatgttt ttccagtgga agtaatatct
    10261 tttataagaa catctgcaga aatatttaat atatttacaa attttatgaa tgttttaaga
    10321 cttggattct ttttaccact ttcgatttga gttatatacc aaggcgatac tccaaccata
    10381 tttgcaaatt cttttgaagt taaatttgct tttttccgtt cttctttaag tcttttacct
    10441 atataattca ttattgttac tatttccctc acttatattt ctttgtattt aatgatgcac
    10501 tttattaact ctagcattgc aatttgttct ttttcagtta atttattaat catttcataa
    10561 cattctaaag gtacagagtt atcaacgcct tctgttgtaa taatatttgc aaataacaat
    10621 tcaagtggta tgtctaaagc agtagcaatt ttcattataa tattgataga tgtatttgtc
    10681 tcagctcttt caatctgacc aatatatgtt ggatgtaaac ctgccttttc tgctagaaac
    10741 tcttgactat atcccaattt tgttctatag acttttattc tatctcctat tatttttcct
    10801 atatttgcca attattatcc ctccacttta gattatattc atagtttaag ttgttttata
    10861 tagactattt atactttttt agtctaaata tagactatat atgtttttat actgaaataa
    10921 gtgctattta tttttgtata gtctgtatct acaataaaca aatatggata aatcatattt
    10981 ttaattagag aaatattttc ataatttcat agccatacta caataataaa acgaattaat
    11041 aatatgttaa ttatagcatt ttttattgta attacatatt agat
//
LOCUS       DAYAOY010000122        11084 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYAOY010000122 DAYAOY010000000
VERSION     DAYAOY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.122"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            18..1346
                     /locus_tag="ACGI6B_003472"
     CDS             18..1346
                     /locus_tag="ACGI6B_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752881.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(1474..2664)
                     /locus_tag="ACGI6B_003473"
     CDS             complement(1474..2664)
                     /locus_tag="ACGI6B_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9752882.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2770..4149)
                     /locus_tag="ACGI6B_003474"
     CDS             complement(2770..4149)
                     /locus_tag="ACGI6B_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9752883.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4180..5388)
                     /locus_tag="ACGI6B_003475"
     CDS             complement(4180..5388)
                     /locus_tag="ACGI6B_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752884.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(6413..7867)
                     /locus_tag="ACGI6B_003476"
     CDS             complement(6413..7867)
                     /locus_tag="ACGI6B_003476"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9752885.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(7887..9758)
                     /locus_tag="ACGI6B_003477"
     CDS             complement(7887..9758)
                     /locus_tag="ACGI6B_003477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9752886.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(9748..10608)
                     /locus_tag="ACGI6B_003478"
     CDS             complement(9748..10608)
                     /locus_tag="ACGI6B_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9752887.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT         3725 a         1996 c         1245 g         4118 t
ORIGIN      
        1 tgaaaaggaa gtaatctatg atgaaaatag cagtcataac aaccgaattt ttaaaagagt
       61 ttgtagataa ttctataaaa aagttaaata ttaatgcaga aattgaaata tatatctata
      121 gagatttttc tcatgttgga gatttatatt tagaaataga agataggttt gatggatttg
      181 cagttagtgg ccccatcccc aaaaaagcta ttacaaaaaa ggctggaact attaaaaaac
      241 ctctagttga ttttggtact gacttacaaa gttactatga tatgtttcta aagcttatct
      301 ttaaatataa tacattagat ttttccagag catactttga cttaacagac tgggcagata
      361 atccaaaaga tatatcatat tatttatcaa atggcacatt tggagactta atggatagta
      421 ttgatgaaaa tgcatctaaa atgagtttat ctgacatagc aaaacttgaa aaaaagattg
      481 caaaaaaaca tattaatcta tggaatgaag ggaaaatcga tttttctact actcgtttta
      541 gtagtattgt tcctatgtta caaaatgcag gagtaaactt tcatttcata taccctgata
      601 tagatttatt aaaaaatact tttaataact taataaaaga tattcattta catagaatga
      661 agggaaatca accaagtgta atatatataa cagttaaaga tacaaattat gatgactcta
      721 ctctagacca agtttataaa atattaaata gtattaaaaa agataaatta accgatttta
      781 taattgaaaa aaatagtaat tcttttaaaa ttttcactaa atgtgaaacg attaaatcat
      841 taactaaaaa ttttactgca tgttatataa aagaatctat tgaaaatagt acaaatatta
      901 aagtatgtat tggatatgga attggtaatg atataaagca agctatgtct agtgccataa
      961 gtgctaataa ggaatctaaa ataaatcctt taaattacag tttcttagtt aacgaaaatt
     1021 atgagacaat aggaccttta ctgtgtgata agttacttac tgtttcaaat aaagtaactc
     1081 cacatataaa tacaattgcc aaaagttcta aattgtctac tcttactatt caaaagatta
     1141 tatctgttac tgaaattcta caaagagatg aagttactcc taaggaatta tcttcatgtt
     1201 taaatgtaac agttagaact gctaatagat ttctaagtag tctcttaaag tctggaaacg
     1261 ctactatatt atatgaaaaa caaaactcaa caaaaggcag acctgaaagg gtatacaaat
     1321 tatttatatc agaaaataaa tattaaaatc caaaatgcat aaaaatattt tatcatctaa
     1381 tattaagtaa aattatatat gactttctaa aaggtatatc tttgcatctt tttaagatat
     1441 accttttatg atatttaata atattttatg ttattatcta ctaaaattat tttctaacca
     1501 ttttactgca caatgagcaa ataaagctga accataaact aatccctctt cattaaaaat
     1561 tgctttagga ttatgcatac caaaattata tccctgacta atacttccca ttccaatcca
     1621 taaaaatact cctggaaccc ttagtaaaac ctctgaaaaa tcttctgaac ccattgcaga
     1681 tggaaagtct attacatttt catttcctat aacttcttta acataagata caatctcatc
     1741 tcctacatcc aaatcattta taactgcagg agtccccatt ttaaactcca ctttagcatt
     1801 tcctccaaat gatttagcta ttcctgtaga tatttcaata attctatttt ttaatttttc
     1861 tctaacaaat tggtcaaatg ttcttaaagt tcctgacata gatgctttat ttggtatagc
     1921 atttggtacg tttcctgcat taaacatacc aatagttaaa actacaggct ctttctgagg
     1981 tttttctctt gaaagtatag tgtttaatgc tatttgtata tgcgacatta tgtttattgg
     2041 gtccactcca aattcagggc ttgctccatg agttgctttt ccttctactg ttatttcaaa
     2101 tatatccgca gatgccatag ctggaccacg tgaataagct acttgacctg tgttaagaaa
     2161 tttacccacc atactgtgaa atgccatagc tgcatcgacc tgaggatttt ctaatatacc
     2221 atcatcaatc attgattttg cacctctggt agtctcttca tctggttcaa atacaaactt
     2281 tactctacct ttaagtaagt gcttattatc cataagtatt ttagctgctc ctaagagcat
     2341 tgctgtgtga ttatcgtgtc cacaactatg agatgcatct ttattcttac ttttaaaagg
     2401 taaatccgtc tcctccctaa taggaagtgc atccatatct gcccttaata ataaggtctt
     2461 tccaccattt tcactgtcta aaacagctac tattccatta ttagacgtca atttcggaga
     2521 atatcccatt ttttctaaag tactggtaac aaaggttact gttttcggta gattgcaacc
     2581 aatttcggga ttttcatgta aataccttct ccatctaata atttcccctt ctattgactg
     2641 cgcttcttta aaaaaatttt tcatattcct tcctcctaat cagatagtca gcaaaatctc
     2701 actttctttt agtaatgaaa tgatgctgat ataaaaatta taattttata tattaaattt
     2761 catgtctttt taaggagttg gaatatcatc tggaatagga catttatatg ttttaccatt
     2821 catagattta tcaaattctt cttttgcctt tttagctatc tctggattat ttaatatttt
     2881 agctccaaac atagccataa tctttgatgc atatatcata cctttttctc ctatagaact
     2941 tcctgcacaa gatgtaaact gccagctatg acctggtgct cctagattcg tacaccctgt
     3001 aaaaaagtat gctgtcggaa ctatatgcat tacatctcca atatcagtag agccataact
     3061 attaaagttt gtaacatttc ctccaccata atatagatga gttccttctg gcaaattata
     3121 tttacttatc atagccttat attgttgtgg gctttgtcta tctaattcag ctgcaaaatc
     3181 caattcctct cgagtccaag gctcttgttt aatctcattc atacattctg atacaagatt
     3241 tgctaaaaca tgattgttta aagtattata acaccctcct aaaaactcta cttcaacttc
     3301 agtttcagtc atcattgcag ctccttttgc tacctttaca agtctttcat atgtactttc
     3361 tacagcttct ctacttaaag ctcttgtata ataccatact gatgctttat caggaactat
     3421 gttgggtgct actcctccat ctgtaattgt ataatgaatc cttacatcag aagtaacatg
     3481 ttctctcaaa taattagctc ccacattagt aagttccaca gcatctagtg cacttcttcc
     3541 attttgaggg tctgaccctg catgtgcagt ctttccctta aagtgaaact ttacagagtt
     3601 aacagctgtt gaagtaccaa ttgtagcttc actaatttta tttggatgaa aatttatagc
     3661 taagtctaac ccttcaaacg ctccacctct agccataaat cctttacctg ttaattgttc
     3721 ttctcctgga catgcataaa atactatagt cccacttaag ttattttcta tcatctctct
     3781 tttaagccct actactgccc ctacatgtgc tgtacataat aaattatgcc cacatccatg
     3841 accatatgca cccaattcaa atgcttcttg ctttgttgaa actttttgat ttaaaccagg
     3901 taatgcatca aattctccca taaaacctat aactggttta cctgaaccaa aactagctct
     3961 aatagccgtg gctaaacctc ctacacctac ttccacatca aatccttcac ttcttagaaa
     4021 gtttgctgta ttttcacatg ctttaaattc tttaaatgct ttttctggat tttcccatat
     4081 tttcttactt aaatcatcaa gtttgaatct ttttttatct atctcattta tagatatact
     4141 tcctatcatt tcctattcct cctataagta tatttactat caaccaaata taattggacc
     4201 tattatacta gcaaatgcca ctgatgcaat ggttacagta acaaatcctc ctataatcat
     4261 ctttggtaat aaataatctg ttgccctttg tttttcttct tcagttcctt ccatagcatc
     4321 aataccttcg ttggaaatta tctgtgttgc aggataagct agcatacaag ttacaccaat
     4381 tgcagctgac attcttggag aatatcccaa tagttttcct acaattgcag taaataatat
     4441 tataaataca gaacctacaa caagtattcc tatagttgga acaatcatcc caaacaatgc
     4501 tgttggagtt accttagcaa gtcctccagg taacatcaac atacaaccaa acattataat
     4561 tccattagat tgagatttat ctaaaattcc tttttctaaa aaacctattc gtgtaaaaat
     4621 caaccctact aacaaacatg ctatattagg atttaaataa tagtttgcag gggtaaatcc
     4681 tggtattaaa gtagccaatc ctacaaaatt accaatcata gcaacaaatg aaagctttgc
     4741 aagatacact agagtagatg attttttacc taaatcttct ttaaaaattc tcatacttgg
     4801 aagtttaaaa ttactttttg actcatcttt caaaaaatcc ccgttatctc ttttaaattt
     4861 taattcttta tttatacaat atgtagttat cggaatacca aagaactttt gaaatgataa
     4921 tactagaatt acaaatgcag caagttcagg tctatttgca gcttctgcaa cagttgttac
     4981 gataatccca gcaacagtag aacctgctat tggaggtgct gctattaaag catactctct
     5041 gccaaataac atactaccca ctgttaaaca gccaactcct attcctacaa tacctactaa
     5101 tgcaattaca accgttttcc attcatgaca taagtcttca agatttataa gtgttcctaa
     5161 gttagttatt aataaagcta taccaaagtt gctcattgca gctaatatcc ctgatgctcc
     5221 tgtaatgtct tttggtaaaa tatttgtcca aaatcctagt aaaaatataa tacatacaaa
     5281 taacaatgtt gaaattgcac cttttgtttt cttagataaa aactcactaa aagcataaat
     5341 cattacacat acagtaaagc aagtaagtgg attccaaaaa ttttccattg ttgtttcctc
     5401 ctaaatataa taatttttta tcaataaaac aataattata aaatgtatat tatgcagtta
     5461 ttcttaacta catatggtat agcttgatat tttttgatga tattatatac gtcctaaatt
     5521 attcctaaaa tatctaaata aatataacta gagttaactc tctaaatatt aactatagtt
     5581 atcaacttac tagatttctt tttgattttt actttctaca taattttacg agttagaatt
     5641 gtacttcaac tactctctat attagtatac atgttggaag acttatattt atttaaatat
     5701 tagatataga atacattttt ataaacgtct taaattattc ttaaaatatc taaaacgaaa
     5761 taataatata cccattaaac caatattatc agaatatact tcactttaag tcttctttat
     5821 atagaaattt tatctattag tgcatcccct cctttgtaaa ttagactatt tctaaacgaa
     5881 tttagatatc ttattcctag gaactattac tagaaacaaa aattatttat tatattaaat
     5941 caatgataag ccatacttta aaattctttc tatacaagta taatttatca tataatgaca
     6001 tctatacctt tatctttata gtagtcctaa attattctta aattgacaat aaaaataatt
     6061 tcactattta taaaaatact tgtttttata ttgtcaaact attcttaata tagattataa
     6121 ttttaaatct tttctttgtc aatatattct gacatttttc cagaaaattt ataaagttat
     6181 atagtgagta aatcatacta tttttatata atgagtaaat tatactatga atatgtctaa
     6241 atatttactt aagtataaac ataaaaaatg tgtattctcc tttttatagt aattttagtt
     6301 gccattactt ttaagaagaa tacacacttt actttaaaga ccctatactc tattttaaat
     6361 gctttactag acactaatta acattagttc ctaacttcta tttatcttat tcctacccta
     6421 ctactatatc cgtagaaaaa tctaaaactt ctccattaga ttttattact tctttgtacc
     6481 aatcaaatga ttttttctta tatctcttaa aacttccttt gccttcatca tctaagtcaa
     6541 catagataaa tccatatctc ttactcatct gacctgtagt tgcactgact aaatcaattg
     6601 gtccccacat caaatatcca aaacattcaa cattatcttc ttcaattgca gacttcattt
     6661 gagatagatg cttacctaaa tattcaattc tataatcatc atttatagtt ccatcttctt
     6721 cgattttatc atatgcccct aaaccatttt ctgtaattaa gataggtaat tgatactttc
     6781 tgtaaatata gttcaaggta aatcttaaac ctatagggtc tattgcccac ccccacttac
     6841 tttgttccaa atatggattt tgaatcccac caaataaaga tttatcacct gataaatttt
     6901 cttttggagt agatacactt gaaaaataat aattaagtcc tataaaatct aacttacctt
     6961 ctttaaatgc ttttttgtct tcatcagtca cttcaatagt tatattattt ttttcatatt
     7021 cttttaattt atattgtgga aacttcccat tgcacattgc atcaatttga taataatcac
     7081 ggtcattatc taaaaatgca tttaacacat tactaggatt acaattgtat ggataaattg
     7141 gatttagtcc aaatacacat ccaactttat tgttgggatt gatttctcta gccaactttg
     7201 caacctttac acccgccaaa gtcatattat accctacatt tgccaatgtt tgaaccttat
     7261 tttctatttc actatactta atacctgcta tcatataagt aaaaatatcc gagtgctctg
     7321 tttgagggtc aatatggttc atctcattaa atgtaaccca gtactttacc ttatcatcaa
     7381 atcttcttat tacagtctca ctgtacttta aatacaaatc tataagtttt ctgttattcc
     7441 atgaaccata tttatgtact aaattcatag gtaattcaaa atgatatagt gttacaatag
     7501 gttctatatt attttctatt aatgtatcta tcagaccttc atagtattta atccctaatt
     7561 cattaggatt ttcatcatct ccatttggaa aaattcttga ccaatctata gatattctta
     7621 atgcactaaa tcccatttct ttaaataaag ctatatcttc tttatatcta ttataaaaat
     7681 ctattccatt atgagatgga taatccaatt tttcttctat tttatcagta attactcttg
     7741 gcatttcata agagccctta gttacaaaat ccataattgc aggtcctttg ttgtcactat
     7801 cccatgaacc ttcacattga tgggctgcaa tacttcctcc ccagaaaaat gtatctttaa
     7861 tattcatgaa ttttccctcc taaaatttat actattacag ttaataattc ttctgattca
     7921 tcatttgatg agattacatc taaataatcg ctagtatttg taactacagt tattatagta
     7981 gaatcaaagc cttcctctat aattttatca gattcaaata ctgctaaagc atctcctttt
     8041 ttaacccttt gattttgtgt tacaagactt tcaaagaatt ttccatctaa gtttacagta
     8101 tctatcccaa tatgaattaa tatttctaat ccttcatcag tcactatacc atatgcatgt
     8161 ttagttggaa atactatagt tatagttcca tctacaggag atataacagt tgattttgat
     8221 tcactaggta taactccaac ccctttacct aatgctccag aggaaaatgc tttgtcatta
     8281 acttccccaa gtgcaattac ctgacctttt gcaacatttc caatactaat agttcttgaa
     8341 ttgttctcgt tgattattag ttctaattct ttttcattat cacccttaac ttcttctttt
     8401 tcatctgaag gtattcctag taaataggaa gctgctgttg ctgctacaaa tgatactact
     8461 acacttatta tcataaatgt aaagttagat acctctcctc caacaagata cgttggtaat
     8521 gcagctaatc cccaaaccat tccatatgtt ttaacttgag ctaaacctgc tattaaacca
     8581 cctatacctc cacctatcat tactgctaca aatggtcttc tatatttcat aaacacacca
     8641 aacactgaag gttctgtaac tcccataagt gcactaaaac ttactgtacc aaataattgt
     8701 ttttgttctt tattcctagt ttttaagaaa tatccaaaca ttgcaccaca aactgcaata
     8761 tcagatattg ttgcagcgcc cataaaaatt gggtcatacc ccaatgttgt cacagaattt
     8821 aaagatatag gcatagtaaa gtttgctgca ccaaagacta ttaagagtgg ttgtaaagct
     8881 gcatacagtg caacaacact ccaattaccc aatgtatcaa ttaaaaacat acatgcatag
     8941 tttaatccat caccaatcca cataccaata ggtccaaata cagttaatgt aactgataga
     9001 gttattatta aagataatac tggaactaaa aaatagtgta gtgctttagg taatattttt
     9061 ttcaaacctt caattataat tcccattgcc catactccta aaagtattgg aataaaactg
     9121 tttgcataag ttgtagactg taaatttata ccaaataaac ttaatccttc tacaccatct
     9181 attgatgaag ataataacgt aactgctaat actactgcta tatatggatt tacattaagt
     9241 ctttttgctg atgctattgc aattaatact ggtaagaagt gaaatgtagc acttcttaat
     9301 gtatctaata tttgatatgt aggactgtca ctctgaagta tgccagacat atttaaaagt
     9361 gctaatatac ttgcaagtat accagctgca agtagtactt caattactga agtcatagtc
     9421 tctgagacta ccacaaagat ggaattaaat atacctttga tacctctaat tccctttttt
     9481 ctttcacttt ttatacttga ttcacttgaa cttaaaccta tcatagacat aaactcgtta
     9541 tatacatcta caacatgagt acctattata atttgataag caacatcatt agaaactaca
     9601 tcaattactc cttttaattt tttaatttct tctgtttgag cgatactcct atcatttaaa
     9661 gtaaatctta aacgagtaac acaatgcgct accgacttta tattttcttt tccccctata
     9721 agttttatta tattttcatt taaatcttta tatttcatct gattttgcct ccagtctttg
     9781 agtcacccta tttatatgca acattaaata agtttcttca cttatagata tatcgacatt
     9841 aaattgtgat tttatatatt ttcttatctt ttgaacactt ccaaatgact ttggataaag
     9901 ttgacttact tgtttatata aatctataga attatcttca tctattgaag cttcctctga
     9961 cataattctt tggataaagt actgtagatg agtagtaaat ctcatataat ttgttgaagt
    10021 ttcatctagc tctacattaa aatgatattt tattatagaa actatatctg ctaaagttct
    10081 caactctaca attgtactat catgagactc tttatttacc tccatattta caaaatgaag
    10141 tgctattgat actgcctcat catcaggaag attaattccc ttactatcac caattaattt
    10201 tagtgcatgc attccaattt caaaatgctt tggataaaat cttttaacat tccataccaa
    10261 aggacttttt ataaattgat tctttttaca tcttttaagt aaaaactcta aatggtctag
    10321 taaggttaag cttagataat cacttgcctt tatatttaaa gttttctcac catattcaat
    10381 tatttcatta ataagaacta tattattgga gtctgagttt tgtagtagat aactaaagtg
    10441 ttctagcata tcgtatgaat ctagtatata tgttctctct atttcatttt catttattac
    10501 ttgtcccact ctttttttaa agccaattcc tttaacaaat acaataattt catttttacc
    10561 tctttttact aaggctacat tattatttaa aatctgaatt attttcatat attcctccct
    10621 tcaataaaaa gaactcaagc atatatgtat agagtatccc tctatttttc acatatatac
    10681 ttgagttctg cctgctaatc agtaacatac tcaaaattat gactacgtcg ccataattaa
    10741 aattcttttt tgtttaattt catattctat atgttatact atccatataa atctgtcaac
    10801 ggtttcctag atattttttt attctataac tacaagctct gttatgcgat tatctgacaa
    10861 ggtttatata ctaaattgat actattttac cgtcactagt atttataata ttgtctgaac
    10921 cattatctat tttactatta tgaattatca tagtaatgct tttaaatata atgaactgta
    10981 ttttaacaaa taaattctta agatatatta acatataaag tcttaagttt agttttattt
    11041 aatcactatt aagatatatt aataggtaaa gcttcaaatt tagt
//
LOCUS       DAYAOY010000123        10892 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYAOY010000123 DAYAOY010000000
VERSION     DAYAOY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.123"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(117..767)
                     /locus_tag="ACGI6B_003479"
     CDS             complement(117..767)
                     /locus_tag="ACGI6B_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9752888.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(1751..2083)
                     /locus_tag="ACGI6B_003480"
     CDS             complement(1751..2083)
                     /locus_tag="ACGI6B_003480"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9752889.1"
                     /translation="MAQPFEVEPKLDELIEWYYSQSEVSIKVITEFHYRFELIHPFQD
                     GNGHIGRFVMLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYLSSLEDFR
                     EVYKMLWK"
     gene            complement(2184..2864)
                     /locus_tag="ACGI6B_003481"
     CDS             complement(2184..2864)
                     /locus_tag="ACGI6B_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9752890.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(2861..3787)
                     /locus_tag="ACGI6B_003482"
     CDS             complement(2861..3787)
                     /locus_tag="ACGI6B_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752891.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(3860..4777)
                     /locus_tag="ACGI6B_003483"
     CDS             complement(3860..4777)
                     /locus_tag="ACGI6B_003483"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9752892.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(4777..5466)
                     /locus_tag="ACGI6B_003484"
     CDS             complement(4777..5466)
                     /locus_tag="ACGI6B_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9752893.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(5874..6179)
                     /locus_tag="ACGI6B_003485"
     CDS             complement(5874..6179)
                     /locus_tag="ACGI6B_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752894.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(7123..7341)
                     /locus_tag="ACGI6B_003486"
     CDS             complement(7123..7341)
                     /locus_tag="ACGI6B_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9752895.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(7342..7749)
                     /locus_tag="ACGI6B_003487"
     CDS             complement(7342..7749)
                     /locus_tag="ACGI6B_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752896.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(7835..8323)
                     /locus_tag="ACGI6B_003488"
     CDS             complement(7835..8323)
                     /locus_tag="ACGI6B_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HFG9752897.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(8528..9625)
                     /locus_tag="ACGI6B_003489"
     CDS             complement(8528..9625)
                     /locus_tag="ACGI6B_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9752898.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(9998..10168)
                     /locus_tag="ACGI6B_003490"
     CDS             complement(9998..10168)
                     /locus_tag="ACGI6B_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752899.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(10340..10783)
                     /locus_tag="ACGI6B_003491"
     CDS             complement(10340..10783)
                     /locus_tag="ACGI6B_003491"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9752900.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         3729 a         1720 c         1045 g         4398 t
ORIGIN      
        1 ttattagatt atatttatta gattatattt agattatgtt taaactataa ttagattata
       61 cttagactac atttagcaac ttaatagata agtatatata gtaacttcaa aacttattat
      121 tcatcttcac agaatctttt aatagttcct tgaatatgat ttcttagttt atctactgct
      181 ttttttgtat ctctgtcttc aatgaatttt aatatttcaa aatggtcttg tactgttcct
      241 tcataattac gattttttaa tggtcgaatt aaatagttca ctactataaa tcttccttgt
      301 ataccaacat atacatcatt taaaatctca ttatttgccc cttctacaat ggctttatgt
      361 aattccaaat ctgctagtgc aagttctaca gatttttctt gacgatagta ctcctcgcat
      421 ttatcagagt atctttttat tacatctagc ttttgttgaa aaacctcttc ctcttcttgc
      481 atcatttttt ctaaagcata tgtttcaatc agtgttctcg tttcacaaac ttgttttacc
      541 atatcatatt catactctgg tacgtacact ttaccatttg tcataatagt aagcactcca
      601 tctttattta gcatttttac tgcttgtaca actggtgttc tgcttacttc aaatttttca
      661 caaaggtcat ttagtaatat tttttgacct acagagtatt caccacgaat aagactttca
      721 ataatcttat gatataaaat ttcatctaaa tttgacttta ctttcactac tctcacctta
      781 tttctattct tttgccatat tatattgaat tactggactt taattacttt tagtaatatt
      841 tttaaagtag aaattcttat ttgatagtac ttttgtacta caatttaaat tattgtactg
      901 taaatcatgc tcttatacta tagattaatt cattgtattg caaatcaagt tattgtacta
      961 tagattaaat cgttatactt caaattaaac tattatacta tagattaaac cattgtactt
     1021 caaattaaac tattatacta tagattaaat cgttgtactt caaattaaac tattatacta
     1081 tagattaatt cattgtactt caaattaaac tattatacca tagattaatt cattgtattg
     1141 caaatcaagt tattgtacta tagattaaat cgttgtactt caaatcaagt tattgtacta
     1201 tagattaaat cgttgtactt taaattaaac tattatacta tagattaatt cattgttctg
     1261 caaatcatgc tagtgtacta caaattaaac tataaatgtt tactatctct taataatttg
     1321 aataatatgt tacaaaaagt atagcacctt tttatttttt tcgcaataaa taaaaaattt
     1381 aaaatttttt attttttatt ttcttatatt atcaatttga aatatgaaag attaagacta
     1441 atagaattga ttcagttaat tgaaaaaaat aggtaccttt tatgaaaatt ctaatgataa
     1501 gcatttttct gatgtaatgg taaattataa atgataaaat taaaaatcac ttttagtttt
     1561 atgaactgtc cccatattaa gaaacaacta agatacataa aaatagttat aaagagatag
     1621 ttaaactata tccactattt tatatagtta aaatagtggg tatagtccaa gctaagaata
     1681 actaagaaat tagcgttaaa tagcatagat aaaaatacat ttatatataa actttttttg
     1741 ctgcaaacaa ttatttccat aacattttat atacttctct aaaatcttct aaacttgata
     1801 aatattcaat taaagggaca taattaccta aactacaaga atttaaggag tttctataca
     1861 attcctcact atcccaagat acaattttta taggaagatt attttctaac atctgtttta
     1921 gcataacaaa tctacctatg tgaccattac catcttgaaa tggatgtatc aattcaaatc
     1981 tataatgaaa ttcagttata actttaatag atacttcact ctgactatag taccattcaa
     2041 ttaattcatc aagctttggc tcaacttcaa atggttgtgc aatattatta aaactctaat
     2101 aatattaata atagctgata taatagcaaa tcattattat cccgagaaga tacaattaat
     2161 aactaccttc ctatccacag tatctagtca atttcagtaa ctttatgatt tagtatataa
     2221 tatataccaa tcacagttat accaactata ataaaaacag aaatcaaact gtcttgagtt
     2281 gtatttgtac cagctaaaat aaattgtctt aaattgataa aaataattga actgacaagg
     2341 gtaacaggta ttgattttct tatcattcca acaacgaatg tccatatacc taatgagcaa
     2401 aatacaataa tacttttaat tgctcctggt atccaataag ataaaattga aatatgaaaa
     2461 tctccaataa ctaaattaaa atacttatcc aacacaacaa cgaatgtaaa acaacagata
     2521 tatccaacta tcattgatga tatcataaga gtcgtaatca aaagtaactt tgataaaata
     2581 agttttttac ggtcaagtgg ataagagaat aaaatcaaga ttgttttatt tgtatattca
     2641 tttattatat atgccacatt caacacagca aataatataa tatagtaaaa acagaaaaat
     2701 aaactgattg tcctaaatgt aacatcataa tcatatattg aagtagattt atcattcaat
     2761 ccagtaaaga caaaaaacat acataagaaa atccccagca ttgaaaataa aaaatatctt
     2821 tttagcccaa ctttttttag ctctagtttc attaaatgta tcatagtgtt tctttacctc
     2881 ctgtacctgt agtttggaag aagtaatctt ctaaagaact ttgtttacgt ccaatgctat
     2941 ttataccaat ttgattttga ataaatattt tggatatttc ttttacatct tttcgtaaat
     3001 catatatttc aatacaactt tccgaagtaa ttttaaagtc ttttattcca aattcttttt
     3061 ctaacaagta tccagcaagc tccacattat ctacatctac ttcaacataa tctgtttgat
     3121 atttgtgtat atctgacata gaaatttctt taataagttt accatcttga ataattccaa
     3181 tagtgtccgc aatttgttct acctctgaaa gaatatgact agaaataaat atagttattc
     3241 cataatctgt atttaactta cggaataatt ctcgcatttc tcttatcccc tctgggtcga
     3301 gtgcatttat aggttcatca agaatcaata attctggttt agtaagaatt gctctagcta
     3361 ttgcaagacg ttgtttcatc cctaaagaat attttgaaac tttcttgttt gattgctttg
     3421 acaatccgac acattccaat acctctgata tatgttcttt gttataataa cccatatatt
     3481 cacagtgtaa atcaagattc tgtttagcag tcaaattctc atataaatac ggattttcaa
     3541 taatagtacc aatctttttt aatatttcaa aatcagtttc tgccactttc ttaccgaaca
     3601 taactatttc tccagaattt ggcttaacaa gatttagcaa cattttcatt actgtacttt
     3661 tacctgcccc gtttggtcca acaaatccat aaatttctcc ctttttcaca tgaatattaa
     3721 cagcattaac aacagcttta tctttatata ttttctttaa ttgattggtc tctatgatat
     3781 agttcatcat actccctcct ttctaaaaac agtatacaaa ataaaattat cttttctctc
     3841 ttttaaatct aacttttttc taacttttca attttactgt aaaaactgtt tttacattag
     3901 gtctactatt tacataaatt gaacctccca tagagtttac aagcccttga gaaatagcca
     3961 aacctaaacc attgcctttt tttctgtcag cagtataagc tcttgtgaaa atatgctctt
     4021 tttcagtttc agaaatacct gaacctttat cctcaatatc tataaaaaca tgtttttcct
     4081 tttcatatag tgaaatccct aaaaatctac catctgaacc atattttata gcattatcaa
     4141 ttaaattttt gagtatacgt tgtaatgctt ctgtgtcaac atttgcatat ataggtctat
     4201 cttcaatatt aacttcaaca ttaaagtttt ctttatctag caaatcataa aactctaaaa
     4261 taatctcatg acataattgt gttagattgg atttttgaag atttaatatc atatctccag
     4321 attggatttt tgccatatta aacaggttat ttattgaatt aacaagctca tttgaatttg
     4381 tttgcatttt ttccacagtt gctctcattg aagaagataa ttcttctttt tgactttgaa
     4441 gatataacat ttcaatataa ccctttaata ctgtgatggg agttcgtaaa tcgtgtgata
     4501 tgtttgtaaa tatttgcaac atcgcttttt cacttctatt ataattaatt tgcctgctgt
     4561 aaaacttttc aagaagttta tttatatcta ctgctgtttc ttttacaatc gaaatatcag
     4621 atggtactaa aatataatta atttcatcat catttgttat gtcacgaagt cttgaattga
     4681 tatacttata atcactttcc attttctttg atttatttcg ttcttttaag tagagagcaa
     4741 atagtacaat cgaaattaca actaaaaata actccattat tcttcctcca ttttgtagcc
     4801 aattccccaa agagttttaa tataattatt tttttctata ccatcagaca atttatttct
     4861 taacctgtta atatgagtat ttagcacatt ttcatttcca taataaggtt ctttccaaat
     4921 agcgttgtat aattgttcct ttgaaaaagc acgtccttga ttagtcgcta gtaatttaac
     4981 aatttcaaat tctgtatgtg ttaagtttaa gtcaattcca ttctttgaaa ctacatattt
     5041 tgatatattt ataatcaaat ctttatactg aataaccata tcttcagagt ttcttgaact
     5101 gctgtacact gttgcacgtc taatattagc tttaattctt gcaagcaatt ctacgattga
     5161 aaaaggcttt gtaatataat catctgcacc aagattaagc cctagcattt tatctgtgtc
     5221 actatctttc gcagtagtaa taataacagg aacaacactc acacttcgta tatattttaa
     5281 tacttcaaaa ccactagact ttggtaacat caagtccagt aaaataatat caaatgaata
     5341 atttttaaat atttcaatag ctgtatttcc atcaatggca gttttaacag agtaattctc
     5401 actaatcaga aaatcgtata ccatttctcg aatattctta tcatcttcaa ccaataagat
     5461 gtccattatt attcaactcc ttttcgtcag tatacttcaa atgatatatt tattaaaaag
     5521 ggttgtcaat aaataactgc ttatgatatt gtatctgcaa tttggtgata ctcttctaag
     5581 ttaaagttct tttcaataag ctttattgaa aaaataatta aaatatagta tactctctta
     5641 tctttatctg aaatatacat aattttcttt tattaacatc tacaattaac tattttatat
     5701 tataaaacac cttttaactc ttgctaaaaa atattttata cataaaaaag aaaccacatt
     5761 ctagttttat acagaacata gtttctttaa aaataatcaa atttggattt taaataatta
     5821 aaaacaaact tttatttgtt tttaattttc caattttata gtagataatt tagttaatta
     5881 gttcttaaat gtttatcttc tttagtaaat ttttttctta ttaagaaata tgcaactatt
     5941 acaacaagtt ctattgctaa aaatacacca taagtagcta tagaatctat gctatctatg
     6001 ccatttctaa agaaaattaa agcaattcct gctgctataa tacctacagc atttttccct
     6061 atagaatttt gaccaaaacc ataaaaagaa gggaaatttc cttttatttc aatgaatgtc
     6121 aagattaatc caatagctat aaatagtact cctacaactt ccataatcat gctaggcatt
     6181 ttgacccctc cttattaata attaactact actataaaaa tatttattat ttttataatt
     6241 atattatata tgcgattaca tgtttttgga agtatatttg gaagtaattt catatttatt
     6301 taacttaatt tttttgtgat aaaatacttc taatttttat atagatatta ttataaattt
     6361 tctagtttat ggagtcattc attctataat tcacaaatat gtatttttta atctattaaa
     6421 gtattcattt tctggcttat aagcatggac ttcttatcta taagcttggt ttttcatcta
     6481 ataagttatt tattctttaa tctatgagct tgatttttca tctataagtc atttattttt
     6541 taatctatga gtttggtttt tcatctataa ataagtcatt ttttaatcta taagtttagt
     6601 tcttcatcta taaatcattt attttttaat ctataagttt ggtttttcat ctataaataa
     6661 gtcatttatt ttttaatcta taagtttagt tcttcatcta taaatcattt attttttaat
     6721 ctatgagctt gatttttatc tataattaat tcattctcta atctattagc ttggttttta
     6781 tttataagtc atttattctc taatctatta gcttggtttt tacctataat taattcattc
     6841 tctaatctat tagcttggtt tttatttata agtcatttat tctctaatct ataagcttac
     6901 ttcttaatct ataaatcatt cattttttaa tctattagct tggtttttac ctataaatca
     6961 tttattctct aatctataag ctttcttttt catctataaa tcattcattc tttaatctat
     7021 gagcttggtt tttcatctat aagtcattta ttctctaatc tataagcttg gttttttacc
     7081 tataattaat tcattctcta atgcataagt aatacatgaa atttaaatat tattttcttc
     7141 aataaattca aacaactctt ctacagttgt atcaaatacc ttagcaatat ccattgccag
     7201 ttttaaagaa ggattgtagc gaccattttc taaatgtaca atagtttcac gtcttactcc
     7261 tactgacttt gcaagttcac cttgtgtcat aaggttcttt tctctatagg atttaatttt
     7321 tgtctttagt aatactgaca cctaattgta tatccccttt ctgtcaaaat atataaatag
     7381 aattaacttc aacaaggatt gaataaacaa aatgactaat aaagacattc ctatatttaa
     7441 atttgacaca tttatgtcca aagttacaag aaatatagat actatagcta cagaaaaaaa
     7501 caatactttt attgcaatat cattaactct tgatagtata cattttgatg cctcatcaat
     7561 taaatcatca tactttttat tatagaccca ctgagctccc atagctaata cccaaataat
     7621 tataggaaga tattcaatac tacttccttt aaaaaaatac ttatttacta atgtagctcc
     7681 aatacaataa gaaattccca atatttctga aataagtgtt gatttaatac cagtttgttg
     7741 tttagacata taaaatcccc cttaaaaagt tatgtattta taacacctct tgttataaat
     7801 atataacttt tttgaataaa tttcaataaa aatattattt tgcaacaaat tcttttatta
     7861 attttacatt agatagaggc actttagatg tcactccttg caatgtcaaa tcttctatat
     7921 taacttcatt taattttgaa cacagttcta agtcaaatat tctttcccag cttaccttct
     7981 tttcaatttg ttcatcttca taaagctcta cagtaatatc cattaaaggt aaatgccata
     8041 aatcaaattc tgataataac acattgtctt cacttatttc tatttcaagt aaaacacctt
     8101 tttttccttt ttctaaataa ccactacatc ttaaatcagg tctcttcgtc caaacccata
     8161 tcatactatc atcaaattca tatattctat ttgtcatttg actatacatc catttgtatg
     8221 gaatatcaaa gtcttctaat ataaaagaat aatcatttga tagatattta ttgtttttat
     8281 tgttattcca aaattctaaa gtctgtatag tataaaattt catatataaa tcaacccctt
     8341 tgtttttcta taagaaggag atggttcaaa cttttatttt gaattatctc ctttatttat
     8401 acacctcttt gtacctatta tttatatatc tctttatatc taccatattt aaataacaaa
     8461 gttttatatt aatttaaatt taaaagagga agctttgtat ttatctagtg ccatctcaat
     8521 catagtatta gttttgatta tagacttttt caacctctga tttattcaaa tcatctataa
     8581 tatcgcatat ttcactcaaa ttgtatcctt tattttttaa ttttatgatg atattaaatt
     8641 tctcttctct tttccctttt gcaatacctt tttcaatacc ttcttgaaaa atttcttctt
     8701 taaaatttct acttttattc ataatatacg cacctctctt taaatttaat aaatagtcct
     8761 cattactacc taattctttt aatctatcaa gtgctctttt aataacataa tccgttttag
     8821 atagtattct aatatctata tgagaaggat tatcaataag tttaccccat attagaactc
     8881 tgtttgctat acaactggat ttacgaattt ttggaagttc tataaaatat aatgcagttt
     8941 tactagaata aatatctgga tgcccgtcca ttgttaatct caggcaattt acataatctt
     9001 ctatgtgatt atattttaca tgatttagta tatgtattcc aacagttttt ccaatttctt
     9061 tatattcttt accttcttct aactttctac catgtacacg actaagataa tattcagctc
     9121 tttccaaata atcctcttca agagctcttt gcatctcaat atttatatgg ttacgttctt
     9181 catcttcagc caaaatatca agtctaggtc ttttctcacc aatgtaatta attgttgggt
     9241 cggtttcaac cttaattatt ctttttattt gagtgaactg tttgatattc ctattagcat
     9301 atggagcatc tttgaggata tcatttataa aatttgcaag taaatcgtaa tcattattta
     9361 aagtaaacaa tgatttaaat acctcatcat actcaggctt agcataccca tcttcagtta
     9421 caacttctcc aacattttca ctagtcaata tttcttttgg aattaaatcc ttgattttat
     9481 ctaatttttc attagaaaaa tcacctctac taagtatgtc atttaatttt ttggtccatt
     9541 ctctgtcctt ttcttcaatt atagttcgtt tatactcttt tgatgtttta ataatcttag
     9601 atttttcttc taatttttct ttcatatcaa cacctctttt ttattattac ccatttcttc
     9661 ataccaaagt ctgaacttct aagtaaaatt tgcattattg aaataagaac cttataaact
     9721 tatatatcat tactaaagtt aagactttaa agagaagcac ttagattaca atagatttta
     9781 gatagctttc tttttaaaac tattgttgat aaatgaatga atataagggt atgtattaaa
     9841 atagagatac tcatcttttt cattgtttaa ataatgaaaa tatattttat ttaatacaaa
     9901 aaagagtgcc ttataaacta aaagttcact tcgagacact cccttacatt ttgtttctta
     9961 aactttacaa tattacatac ttatttatta atttaatcta tgcatactta acccaataat
    10021 aaagtacata tatccaactc attaagccat gaaaaatagc ccatggaata gatttccatg
    10081 cagtataaga aataatcatt gctaaacaag aaccaaaaga aatacccttt ttaacagttg
    10141 attttatagt attattattt gaatccataa ttaatcctcc aaaattcaaa ataaacaagt
    10201 tattatatat cttatttgaa tataatacaa tataacagct tctaaataaa ttatactctt
    10261 attatacata atttataaat tataattccg ataattatat gaattaatta attatattaa
    10321 cctaaaaata ttatcctatc taaaagttat atgatagata caacatgtca acacattgta
    10381 attctccttc aaagattggt tctggataat tatctacaaa aaagttttta attttatgtg
    10441 aataaacaaa tccaaacttc tcataaaaag gaactccgct ttcagatgta ccaacaacca
    10501 ttgatgaata ttttttacta taaacatcta aaagatgata aatcatcttt gaggcataac
    10561 ccatattttg aaaatgctca tatgtagcaa tgttttttaa ttcacatatt tctttggaaa
    10621 tttcatttac aactgctata caagtaagtt ctttattatc atacaatgcg tacatagttc
    10681 ctttattgag atatttatca atcatttctt cacatgggtc ggctaacatt aaaaggtcaa
    10741 ttcgactttt tttatttatt tctatagttt ttatttctat cataagttct ccaccctcac
    10801 tttattcaaa ccttaattta acaaacttct tacagtactt atttaattca ttttgatata
    10861 tatttttact ttttaaaata tagtgataat tc
//
LOCUS       DAYAOY010000124        10843 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAYAOY010000124 DAYAOY010000000
VERSION     DAYAOY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.124"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(584..1318)
                     /locus_tag="ACGI6B_003492"
     CDS             complement(584..1318)
                     /locus_tag="ACGI6B_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9752901.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2329..2967)
                     /locus_tag="ACGI6B_003493"
     CDS             complement(2329..2967)
                     /locus_tag="ACGI6B_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9752902.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2970..3827)
                     /locus_tag="ACGI6B_003494"
     CDS             complement(2970..3827)
                     /locus_tag="ACGI6B_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752903.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3829..4203)
                     /locus_tag="ACGI6B_003495"
     CDS             complement(3829..4203)
                     /locus_tag="ACGI6B_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9752904.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5365..6555)
                     /locus_tag="ACGI6B_003496"
     CDS             complement(5365..6555)
                     /locus_tag="ACGI6B_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFG9752905.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6571..8526)
                     /locus_tag="ACGI6B_003497"
     CDS             complement(6571..8526)
                     /locus_tag="ACGI6B_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFG9752906.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8543..10588)
                     /locus_tag="ACGI6B_003498"
     CDS             complement(8543..10588)
                     /locus_tag="ACGI6B_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFG9752907.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPDGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGEIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
BASE COUNT         3677 a         1889 c         1239 g         4038 t
ORIGIN      
        1 atggtttaaa taatcttctt tactttgtat tatgatacat gctttaaata tttttacgca
       61 ctttatatta taatacatgc tttaaataat cttattcgct ttgtattata acacattatt
      121 taaatatttt tactcactct gcattatagt acattattta aatatttttc ttcgctttgc
      181 attatgatac attatttaaa tatttttact caccttgtat tatggtacat tatctaaata
      241 tttttcttca ctctgtatta tgatatataa cttaaatatt ttacctcact ctacaatatt
      301 atatataatt taaatatttt tcttcacttt gtatactaaa gtttatttaa agtattttct
      361 ttcccattat actatgctgt taaatatttt tcaactgaag tattgtagaa ccttaaaatt
      421 actataaaaa actatattct aaaatacttg taatactacg tatcaccctt aacacttaag
      481 tataatttta aagcaaaatt cttattactt atatttaatt ttgattctac atctttagat
      541 ataattttaa gtaatttaag aaattattgt aaatcatata gtactactca ataattaggt
      601 ttttcaatgt tccaattatg tttctttgat ataatccacc atgactaaaa actatcgttt
      661 ctatatcata attacttata tttttaatag catcttttaa ctcttgtttg ttgctgtgca
      721 ttacatcaac aggcttaagt tctccatttt ctacctgtaa caaatctcca gcaataagta
      781 ctttagactc ttttaaataa agacatatgt gacctctagt gtgacctcct gtatgtataa
      841 cttctatttc ctcacctaga ttcaattttt catggttatc taatgacaca tctactttag
      901 tatatgatga tttaaatcca agtcccataa catttagcat actaagcatc ttatcatcaa
      961 ttttatctac catttgttcc aacatataaa gtttaaaagg agtttcttct cctgttatat
     1021 agctgatttc acttttgtat gcatatactt tgatattatt cttagatttt tctcttagat
     1081 aatttatgtt tcctatatgg tctatatcat gatgagtaat tattatattt tttaacttat
     1141 caaagctcgt gttactttta ttaagagcat caattattaa atcaccttga cccggaagac
     1201 ctgtgtcaat aagtgttgca tcatcttctg tgtagattac aggaatatac ataactttat
     1261 cttcaccgaa tacatttgat gaaattttta aaacatctaa tccctttgct aaattcataa
     1321 tttttctcct tataattact taattagagg tcaatttaat taaagaaatt gagaaaatgt
     1381 agattttcct ctagttatat tttattaatt aaaaattatt aatacctagt atatcactta
     1441 agttgaagga ttactcgact tgattttttt gttattttat ggtatagtaa ttaatataga
     1501 agtaagtaat tgcaagcttt gcaattactt ttattttttt ctaataacta ttttattttt
     1561 gataattcgt ataaaattaa aagtaaagta atatggaaaa tatgaatata gaaaaacttt
     1621 aataagaaaa taaattatga ataaataaaa gaaacgcaat actgtatata gatgattatt
     1681 tagaaaaata aaatctcttt taataaaaat aagcttcaaa ataaactttt agttcataaa
     1741 gcttaggagg aactaaattg agtgcaagtg tatctactgt tcaaaataaa tagttcataa
     1801 agcactcttt tttatattaa atcaattata ttttcgtcat ttcagcaatg aaaaagaaac
     1861 ttatctctat tttgagacag cttcttttta ttaacttcct actttttatt agttttctaa
     1921 aattgaaaag ctttatttat aggattcctt agatttatat tttgacgata taatttttta
     1981 aaaacatctt aaaagatggt atacaataca cacaatcata ctcataattg aaaaataatc
     2041 aatggaaaat aaaattactg gatattatct attaaaaaat acaaattgat tcacaagaaa
     2101 catataatct ataatattat gatgttactc tctcgcacca agtaacttta ttttcaagta
     2161 accttttaaa ttcataccat ctaacttcac tcaataaaaa tggcaatata aatatgtact
     2221 ctatttttta cttttaaata tctgacaagc tttatatact attggaagta aaatacaaag
     2281 taaaacagcc caatctggta aatgtggcat ataaaagtcc cccttacctt atacaagctc
     2341 cttattacta aaaattttaa tagaaataaa cattgaagct aacagtataa ttaaacttaa
     2401 aaggaacaat acaatattca ctatcatgga tggatactga tatataaatg gaatttcaaa
     2461 attgctgtga gctatagtta atggtaatac aaatgatgaa gccatgatta tcaccacgaa
     2521 aaagaatttt gttgtttcat atcctaattt atattgaata ggcatataaa ctccataaat
     2581 aatagaactt attaataaaa caatcaaaat atatgttaag tcaaaagttc ctatttgtgg
     2641 aataagtaaa ttttcaatcc agtaaactaa tgtacaatag ataaatacca ttgcaaataa
     2701 aacatactta gattgaacta agccacgtct aggataagga gttgaacaaa gtaatacaga
     2761 tgcttttgaa tattgactct cctttaataa tagatactgg cacagcataa attctgaaaa
     2821 tataatggat agtacaaatc ccaatatttg tgcaaatgga ggtaaacgcc acaatgtaaa
     2881 taatgggatt cctattgcaa taaacaatat caacaataca tattttttta caatcagaat
     2941 atctttttta actaaatgaa tcaacataat tactctctcc tttcaatata tgcaagcata
     3001 atatcttcaa ttgtaggacg ctctattaaa atatctggca tatttttttg cacttcatta
     3061 atttttgagg taatccctat aaaaccataa cctgtaattt ctaaatttaa aaataatttt
     3121 ttgatttcat catttagaaa ttgaatatcc ccttttactt ttctatgttc gtccaataga
     3181 gtatcttttt cacgctctaa tataatacgt ccattgtcaa tcataacaat catatctgct
     3241 attttgtcta aatcagatgt tatatgagtg gaaaagaaaa caccacgacc acattttttc
     3301 atatatcctg ttagtatgtc aagtatctga ctacggatta gtgggtctaa accacttgta
     3361 ggttcatcca taattaaaaa ctcagcttta tgcgataatg ctaatgctaa tgcaaatttc
     3421 attttcattc cttttgaaag tgtatcaatc ttttgttttg ggttaagatt aaaacgcttc
     3481 atattttgtt gataatcttc ttcacaccaa ttatcatatg aaggtgcaat aatacttttc
     3541 atttctgcca ttgacaactc ctcatagaaa tatgaatcat caaatacaac tccaattcga
     3601 ttttttaatt ccttctcatt tccatcgaaa tctttaccaa agatttttat ttttccacta
     3661 tcacgcttta cgacatccaa tatcgaacgg attgttgttg tttttcctgc tccattaaca
     3721 cctataaatc cagttataca atcttctggt aatgcaaagc ttatattaga taatgaaaaa
     3781 tcggtataag tcttatttaa gttataaact tctaaaatat tttccatatt aatcctcctg
     3841 atatagaata tccatcattt cttgtaactc tatctgtgta attcctagtg cttttgcatc
     3901 cttaatcata tctgccattt tttgttcaac tatacgtctc tttgagtctc ttagtatttc
     3961 taaatttcca gttgaaataa atgttcctat tccaacttgg cgtgtaagaa agccttcatt
     4021 ttcaagttct tcatacactc gtctaatagt caaaatacta acttttaaat cattagcaaa
     4081 atttcttatg gatggcattg catccccttc tttcagttct tcagtaaata ttgcttcttt
     4141 aatttgctct ttaatttgtt ggtataatgg cttatctgat gtgttagaaa tgagcacttt
     4201 cattttttat cacccccttt ttatcatttt cagtgtgatg ttgttatata cacactatac
     4261 acttagagca cttttttgtc aatataaaag atgtaaaata ttactatgac aaaataaata
     4321 tgaatgaaat attagagatt gttgatagct ataaacactt tttaaaacca cataagatag
     4381 aatgtattaa atatttatta aaagtaagaa ttgaatatct aaaaaaaaga ggaatcctgt
     4441 atgaatgatt tgtttgatga taaattaagt tataaactat ttataaaaaa cataaaggaa
     4501 taaatccatt tctaattaca aacatactaa aaaatttaga tagtaataaa tagatagtaa
     4561 taaatagata gtaataaatg ggatgaattt ataagaattt ggatagagga aagagttatt
     4621 caagagtgta gactagaggt aaactctata ttaaaaaaat ttaaatttgg atattatgat
     4681 gcatggaata tttgcagaag taaccttgct ataagtatag aagatttttt tgattacaaa
     4741 agataatact ctaaaatgtg aggatttcaa cattcaatac tgtactttaa ataatatcac
     4801 tgcaaaatac catccacctt ttaccataat tgaataccct aattatttta aaaaagaaaa
     4861 cattagaaac agaaaacctt ggatatataa aataaaaaat actaacttct aaaacacgga
     4921 tactctttga taaataaaac aaaaaataat tttaataatt aaaattggct atctcaaact
     4981 agagataagt ctctttctca cttttaaaac gttgaaaata tattcgattt cgtacaaaaa
     5041 agagtgcctt agaactaaaa agttcacttt gtggcactcc cttttttctc ctctttttaa
     5101 ttttttataa aaattcttca aaaatattat taaataccct atattataca taaatcaaat
     5161 taaagttgca ttttagcaaa taaaaacaaa ttataactat tagcatagca aataaaaaat
     5221 ctgctctctt ataaaatcaa attataatta taaaaagaaa atattcttat aacaaattag
     5281 ctaatataaa attattttat aaattaatac tactttttag cttctcctat aaattaatac
     5341 cactttttta actttgaaac ctatctacaa cattggtacc ataagaccag ctaccacaat
     5401 agaaatcata caagccatac caccaactac cagcttaggc ataatcttag acatcaaata
     5461 tgctctttct tcttccgttt ctccaacagc attacacact tcatttgaaa ttaaatatgt
     5521 agttgggaat cccaacatct gacaaaaccc tactccaaaa gcaagaggtt tagaaccgat
     5581 aattttccaa cctggtaaca ccttcatcat aaggaatata ccaatgatag aagctgcaaa
     5641 catacccaca attggtacaa aaagactaat taaatctgtc aaagaaactt ttgctaaaga
     5701 aggaataaca gctgcaaaca caaccatgtt gataaatccc ttgatttttc ctttttccaa
     5761 aatgtctttt ggcacaatac caatacgtgt tacaataaca ccaacaacaa gacatacaat
     5821 agaataattt acatgaataa tctctgaaag ttcataccct agataagaga ataatgctat
     5881 aaaaaatata caaacatttg tagaataata tttatcgtat ttttcacaga aagcttgttt
     5941 tacttttgtg cctgtattct ctgcttcttt cttatcagta tcaacatttg ctaaatcaat
     6001 gccttttgat tttgcttcat gatattctac taaaagacgg tttgcctctt gtaacgctgc
     6061 tcttgaagct attggtgtac caataaactt ctgaattgca aatacaactg ttgctgtagc
     6121 tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc ataatagtag tagcaggtaa
     6181 agctccattt ataactggta tgcttgtcaa tactgttgat tttccaaaca aaggaataca
     6241 taatgagaca cagactataa ctgctacaat tccaagccat gctgtcaata ctgttctcca
     6301 ttcgtctata aattgtttaa cattcaacat tgttcccata ctaaacataa tcattggaac
     6361 actccaacta gctgctgcgg tcatacctgc tttttcaata atatcagctg gtattgcttt
     6421 tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat acagctgata cttttgcttt
     6481 tgttttgctt gcaattaaat ccccaatagc aaataaaact aatacgacac taaaccataa
     6541 taacatatca ctcatagaat aaccccctta ttataaatat ctttcaaacc aatctacaat
     6601 agcatttgct cttgcaattc tattagatgg tcttccattt ctagacaatc catgtgtatc
     6661 tccgtggaac aaatacattt ctgtatctac accaagctgt tttaatgctg taaacatttg
     6721 gaaagtatcc cccataaaac atctataatc cctatcacta tgtaagaaca atgttggtgt
     6781 cttaacattt ttcacatatt taataggtga atgattccaa agaacctcct catgattcca
     6841 aggcgtctta tctccacaag tctgcttcat aattctatcc ccaaagtcaa taattaaaga
     6901 agctgtaatc aagttactaa tacctctttg agaaactgct gctttgaaac ggtctgtatg
     6961 accaattatc cagtttgtca tataaccacc gtaacttccc cctgcaaccc ctaagcggtc
     7021 tgcatcaatc ccaggatatt tttcaataca agtatctaca aattccataa gatgctcata
     7081 gtcaacatac ccccatcttt ctaccatttc taagaatttt tcaccataac cgtcaccacc
     7141 atgagggtta caataaatca catacattcc ctttgatgtt acacactgct tcaagtgtgt
     7201 aaacatatag ccatatgccc atttaggccc accatgaatg aacaatacag caggatattt
     7261 ttcgccttct ttagcatcta taggtggtat aacaaaacca tcaacttctt ctttattttt
     7321 tgctacaaac gtcaattttt caggtaatga aaccttatga gtatctaaat atggtttaga
     7381 aaaagcagtc atttttttca ttgtacctgt agtcatatca atgctatata tttctgctaa
     7441 ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg taaatatcaa aagatgtaaa
     7501 agcatctact tttgtgatga tacgtatatt acctgtcttt gtatcaaact cattaaattc
     7561 tgtcatagtt ctgcaaattc ttgcaccaat aaatttatct cctataactt tattactacg
     7621 tccaccacca tatactgcat ctacatccag taatgtagca tacataccat ctggactagc
     7681 aatcatattt atttcactag tactcaaatc aagtataaaa atatcatgat tctgtgtaat
     7741 agaacggtct aatgttgaag cttgcaacat tactttatct ttgccaatat attttgccaa
     7801 acttatttgg tactttcctt gtggtacaag ttctttggtt tcaccactgt ctatatgata
     7861 ctcccaaagt ccagaaaaag gagttgcaca atcttcataa atctgaccag tatataaaat
     7921 tctgttttta tccgaagaaa catctatacc tgctacttca caaaattttg gtgtaatggc
     7981 ttttgttata ttttcacctt cttgatataa ttctaatgtt acacgtcttc tttgagaaaa
     8041 attttctcca tctcttataa aaggtttatc tgtaaatgtc caataatcta caccctctac
     8101 tgccctatct ggttctgatt ttttagaatc cgttggattt tttgttgtca gtaaccacaa
     8161 tttatttcca agtgatacag gtacgctaca agcttttgga atagaaaatg ctttttttgc
     8221 ttctcctcct tctatagaaa tagtgtaaaa atctgttgta cctgcttttg gcttatttct
     8281 tttagatgta aaaagtagtt ctgtattaga aatccaacta aatgatgtta catcttttgt
     8341 ttgagtcata tgtatttgtt tttctgtttt gatgtccatc acatataagt catgttcata
     8401 gcagtctttt tcctcgttga tatttgatat aatataagca atatgctttc catcaggtga
     8461 acaagaaata tcatgaggga atatatattc aaaaatatct gaactttcaa tctttatttt
     8521 ttccattttt atattctcct ttctactgtc ctatgttatc tttttttata tataccgaaa
     8581 accaatctag catttcaact aatctttgta gcttttgttt tggtctacca gaacgagaca
     8641 gttcatggtt ttctccttca aatagacaaa gttttgtagg acacccatga attttcaatg
     8701 ctgaaaacat ctggaatgct tcttgcatat agcatctata gtctttttca ccatgtaaaa
     8761 ataaagtcgg tgtaacacat ttatcaacat aagcaagagg tgactgtttc cattgaagct
     8821 cgtggttttc catatgagaa gcacctgttt gtcctttgca gaaataataa ccaatgtctg
     8881 ttgtaccctc catgccactc cagtttgcaa tagaacgctg agcacaagca acagcaaaac
     8941 ggtttgtatg accaattatc caattcgtca taaatccacc ataacttcca ccttcaacag
     9001 ccaattttgt tttatcaatg tctgtatatt tttcaagtac agtatctgta aattccataa
     9061 aatctatata atcaatttct ccaaactgtt tcctaatatc tgcaaaagca ttgccacgac
     9121 cttcagaccc ccttgggtta cagaagaata caaagtaacc tgcacttgta aatacctgta
     9181 tctcatgcat aaaatatggt ccatatgtca atcttggtcc tccatgaatc gccaatacag
     9241 ctggatattt tttccccacc tcataatcta ctggtttata tacgtatcct tgtatttccc
     9301 atttagaact tccttttgac tctatgtatt ctggcacaga aattttatat tcctctaaaa
     9361 tatggtcatt taaatgtgtt ttcttacaca atgttccatt ctctactgta taaacttctg
     9421 gtaatccatt tcctaaacaa cccacacata caatattgcc atcttttaca tctataaaat
     9481 caactgaacc tgtctgtgtt actttttcct gttttcctgt tggaatatgt attctttcaa
     9541 taatgcagtc catatctact gtagaagtgt aataaatata ttctccatct acctttgttg
     9601 tctgaccagc acccatttta acatcagatg ttactgtatt tccaatacaa tgatgttgat
     9661 gccctgaaag aagtttcatt tctttcgtat caatatcaat agtataaaaa tctccatttt
     9721 ctccatttcc ataagataaa gcatcagtta ggtttagtat cacttgattt tgatataatt
     9781 caaaaccttt tataatatac ttatcttttt ctaatataca gacatttgtg tttgtgtttg
     9841 tatcaaatac atagacccca tttttcaaac cttttacatc tttaaattct tgccctatat
     9901 ataagatttt tccatcactt attttcatac ctacaacatt aaacataggt gctgtaattg
     9961 ccttcaactc atttatatta ctatcgtata catataggcg ttttcttttt ctgctaatat
    10021 atccctcccc attgacagca taaggaattt ctttgaaatg ttcataatgt cttttgttat
    10081 aagcatttat ttttttctgt tgttcttctt ctggcaatcc ctctacaata ggtcggtcat
    10141 tgtcataaga atcaataact gcatacaaac cttttcctat aggattcaat ttaccacctt
    10201 tgatagggag tctaaaaagt tcttctgctt ctccaccatt taaagataaa gcatagtaac
    10261 tcatgcattg cttaccgttt ttgacagctt cttttacttt ttcatttcgc aaagcaggga
    10321 acaagatagt attttcatct tcccaggcat atgctccaac agagtttata gacgttattt
    10381 gttttaattc gccagtttta ttgtttaata tataaacttg gcttgtgtaa ccattctctt
    10441 tgatatcagc tttttttaca ataaatgctg tatatcctcc atctggtgat atttggcaat
    10501 cacttataaa ttgatattcc aaaaatgttt cactcgtaaa tagctttttc atagtatttt
    10561 ctatcctttc tttagtaatt tttttcaaac tccctaccac ctctttcatt ttttatttta
    10621 aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt tataaaggtt atttttttag
    10681 gaagccttct ggcttccttg taatatttgt aatttaaagt atatttaatc catgcttaat
    10741 tcatgcttaa ttcatactta atctatactt aatctatact taatctatac ttagtccata
    10801 cttaatctat atttaattca tacttagact tatttacact ata
//
LOCUS       DAYAOY010000125        10700 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYAOY010000125 DAYAOY010000000
VERSION     DAYAOY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.125"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..321
                     /locus_tag="ACGI6B_003499"
     CDS             <1..321
                     /locus_tag="ACGI6B_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9752908.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
     gene            454..1824
                     /gene="hydA"
                     /locus_tag="ACGI6B_003500"
     CDS             454..1824
                     /gene="hydA"
                     /locus_tag="ACGI6B_003500"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFG9752909.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1842..3248
                     /locus_tag="ACGI6B_003501"
     CDS             1842..3248
                     /locus_tag="ACGI6B_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752910.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            3320..3766
                     /locus_tag="ACGI6B_003502"
     CDS             3320..3766
                     /locus_tag="ACGI6B_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFG9752911.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3805..4599
                     /locus_tag="ACGI6B_003503"
     CDS             3805..4599
                     /locus_tag="ACGI6B_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9752912.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            4589..6880
                     /locus_tag="ACGI6B_003504"
     CDS             4589..6880
                     /locus_tag="ACGI6B_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9752913.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6881..8194
                     /locus_tag="ACGI6B_003505"
     CDS             6881..8194
                     /locus_tag="ACGI6B_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752914.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8230..9315
                     /locus_tag="ACGI6B_003506"
     CDS             8230..9315
                     /locus_tag="ACGI6B_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFG9752915.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9551..10594
                     /locus_tag="ACGI6B_003507"
     CDS             9551..10594
                     /locus_tag="ACGI6B_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752916.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQEM
                     IVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINV
                     LKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMK
                     EDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEI
                     QIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATS
                     KNWSDIVKLKSVK"
BASE COUNT         4312 a         1119 c         2174 g         3095 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg acgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctatgcaggt
      181 gaagctggaa taaaaactat aggatttggt ccttctaagg aaaatcttgc tcacacaata
      241 gatgagtata tagagcaaga acagttattt ataggaacag aaggttacta tggaatatta
      301 aaatctgtat acggaaaata atatttagtt cacaataaat tatttaaata tagtctagag
      361 gtgctgaata tagcaccttt agaaaataca attttaaata aagttttaca atacttagaa
      421 aatactttaa agcttaaaag ttaggaggta tatatgggaa caatactaag aggtggaact
      481 ataattacct ctgataagac ttatataagt gatttaagga tagaagatga aaagatagtt
      541 gaaataggaa ataacttaga aataaatggt gataaggtta ttgatgctac aggtaaaatt
      601 gtaattcctg gtggaataga cacacatact cattttgata tgaatgcagg ttctattaca
      661 actgcagata attttgaaac aggaacaaga gcagctatag ctggaggaac tactacaata
      721 ctagattttg cagaagccaa tgagggggaa aatctactgc aaggtgtaga agcatatcat
      781 aaaaaagcaa gtggaaattg ttattgtgat tatggttttc atatgacaat tacatgttta
      841 aataaagata catttaatca tatggaaaaa cttatttcag atgggatagt atcttttaag
      901 atgtatatgg catatgatgg catgaaagta gatgatggaa caatatacag ggttttaaag
      961 aaagcaaggg atttaggttg tatagttgga tttcattgtg aaaatgggga tttactagat
     1021 gtattgatag aagaaaatat acaaaatggt aatttggagc caaaatatca tccacttaca
     1081 agacctaaca tagttgagaa agagtctgtt tcaagacttg ctgatataac taaattatct
     1141 aattcaaaat cctatgtagt ccacttaagt tgtagggagt cacttgaaac tgtaaaaata
     1201 gctagagaaa aaaatataga tatgatagta gaaacatgcc cacagtatct attattagaa
     1261 gacaatttat ataataagga taaatttgag ggtgctaagt atgtaatgtc tcctccttta
     1321 agaaaaaaag aagacataaa ttacctttgg gaaggattgt ctagtggaga tattcaaaca
     1381 gttggcacag accactgttc atttaatttt aaggggcaaa aagacttggg aattgatgat
     1441 ttcagtaaga taccaaatgg agcacctggt gttgaacata ggttagcact tttatataca
     1501 tatggggtct tagaaaatag aataagtgta aataagtttg tggaagttac ttctacgaat
     1561 gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga tagctgtagg aagtgatgca
     1621 gatatagtca ttttaaatat caataaagaa gaaacaatta gttataaaac tcaaaaacaa
     1681 aatgtagatt atactccata tgaaggattt aaggttaaat gtaaagttga agatgtattt
     1741 ttgagaggaa atcatgtagt acaaagttgt aatgttaaag aacatccaac aggtcaatat
     1801 attaaaagaa aaattactaa ataatgcggg gggaaataag aatgatggat atacttatta
     1861 agaatgccat aatagtaact gtgaataaag agagagaagt tatttttgat ggggcattag
     1921 tagtaaaaga taacaagatt gctgatattg gaaattctaa agagatagaa agtaaatata
     1981 ctgatgttaa aaaaattata gatgctaaag gtaaagtttt attcccaggt tttataaata
     2041 cacataatca tttattccaa actttattaa aaggattggg agatgatatg gtacttaaag
     2101 actggttaga aactatgaca tttccagcag ctaactattt agagcccaaa gatacttatg
     2161 atgctgcaat gcttggatgt attgaaggac ttagaagtgg tataacaaca atggttgact
     2221 acatgtatcc tcatagtaag ccaggtcttt gtgatggaat aatagatgct tataaagaat
     2281 taggaataag aggaatactt ggaagaggat gtatgaatac aggagctcaa tttggagttc
     2341 atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga tgtaagaaga ttgtttgaaa
     2401 aacaccataa tacagaaaat ggaagaatca aaataggagt agctcctgca gcaatatggt
     2461 caaattctca agaaatgctt gaaatgctat ggagagtagt aaaagaatat gatgatgctt
     2521 tatttacagt acatatatca gagactcctt ttgatagaga agcagcaaaa gaattgcatg
     2581 ggcaatatga catagatgtt ttagaaaaat taggaatact tggaccaaat gtgcttatgg
     2641 tacattgtgt gtatctaact gaaaaagata tggaacttac taaaaaatac gatatgaaag
     2701 tgtctcataa tacagcaagt aatatgtact tatcttcagg tgttgcacca gtgccagaaa
     2761 tgcttaaaaa aggaataaca gttagtttag gagtagatgg agccgctagt aataactctc
     2821 aagatatgct tgaacttatg aaattaacag cacttcaaca taaagttaat aagtgtgacc
     2881 cattagcaat gtcggcagaa aaagttcttg agttggcaac tatagatgga gcaagagcta
     2941 ttggaatgga agatgagata ggttcattgg agataggtaa aaaagcagac ttactaatat
     3001 ttaatccaat gctttcacca aaagcgatac ctatgcataa tccggtatct acattagtat
     3061 attcttctag tatgaaaaat atagagagtg ttatagtaga tggaaatata ataatggaag
     3121 attcaaaaat attgacagct aatgaagaga aagcattaaa agatgctcaa gatacagctg
     3181 agagactttg tataagagga actataaaaa atagaatgga aggtcataaa tggaatagcc
     3241 tttattaata aatgtaattt attcataaaa aagtatagta aaactttagc ttaaaaatat
     3301 gagttttgag gtgatttatt tgattatcaa atgtaccata aataataaag aaaaagaatt
     3361 aagtataaga ccagatgatt ttttagcaga tacattaaga gaaaatggat atgtaagtgt
     3421 taaaaaaggg tgtgatacag gctcatgtgg gttatgtaca gtgatggtaa atgacaaggc
     3481 cgtgctttca tgtaattatc ttactgctag agtagaaggt cagcatatta caacacttga
     3541 gggtgtcttg gatgaagcaa agaagtttgg agattttctt gcagacgaag gggctgacca
     3601 gtgtggattt tgtgctcctg gatttataat gatggtcttg gcaatgaaaa gagagcttca
     3661 aaatccaaca gaagaagaag ttatagatta tgtaaatggt aacctttgta gatgtacagg
     3721 atataaaggt caattaagag caatacataa atatttaaac acttagactt ttaagtgaaa
     3781 attaagttga tatggggaga gagtatgata actataaagg aatatgtagt tccaaaaagt
     3841 ttagatgaag cttatgaact gctaatatca agaaaaaaca acataatact tggaggatgt
     3901 ggatttttaa aactaggtag taaaaatatt ggttcagcaa tagatttaaa agacttagct
     3961 ttagattata taaatgaaac tgatgatagc atattaattg gagcagatac aagtttaaga
     4021 actttagaat taaataaggt tattaaaaat tactgtaatg gggtaatttc aaacgctgtt
     4081 tctaatatag taggagttca atttagaagt ggagctagag taggagctag cgtatttgct
     4141 aagtatggat tttcagattt aataccttca ttactagtgg tagatgcaaa ggttaagtta
     4201 tataaaaaag gggttatgaa cttagctgat tttttagaaa atgagcttga aaaagatata
     4261 ttaatagaag ttatattacc taaaaaagat gctataggtg tatttgattc tataagaaaa
     4321 tgtacaggtg attttgcagt attaaatgga gcaatgttaa aagaaggaag tatttataaa
     4381 attgcgatag gcgctagacc aagaagagca aaaattgcct ataaagcaag tgaaatatta
     4441 agtttagaaa atgatataga aaaagctggt gaagtatcaa gtgaagaact ttcatttgga
     4501 agtaatataa gaggaagtaa agaatacaga aaagatatga caaaagcctt agtagtgaga
     4561 atgtataata gtattggagg tgagtgtgat ggtaaataaa gaggtaagaa aaatagatag
     4621 tgaagccatt gttactggaa aaccaatata tacagaagat ttaatttttc ataaagatgt
     4681 tttaacaatt aagcttctta gaagtccaca tgcttttgcg aagataaaaa acatagatat
     4741 aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc acttacaaag atgttccaaa
     4801 ttatagatat tcaatggttg gagagtcata tccagaagca tcacctcatg accaattaat
     4861 acttgaagac gtagttagat atgtaggaga tgaagttgct ataatagccg ctgaagatga
     4921 aagggctgct attaaagcta tgaaacttat aaaagttgaa tatgaagtaa tgactcctat
     4981 attggatgca agagaatcgg aaggaaataa aattttaatc catgaagaag atgatttgtt
     5041 ttgtccattt gattttggat tggatgctac aaaaaatatt gtatcgagag aaaaaatggg
     5101 taaaggggat gtagaaaaag agtttaaaga aagtgatgta atagtagaac attcttatat
     5161 gactcaatca caagcccatt gtatgatgga aactcataga gcttatacgt atatagatac
     5221 gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct tatcatatga gaagacaggt
     5281 ttctaaagca ctgggaatac ctctttcaaa agtgagagta ataaaaccaa gaataggtgg
     5341 aggttttggt ggaaaaaaca tagctgtaac agagatatat gcatcatttg taacttggat
     5401 gacaaaaaga ccagcaaaac taatttatac tagagaagaa acttttgcaa tgactaatac
     5461 tagacatcag atgttttttg atataaaaat tggggcagat aaagaaggaa acataaaagc
     5521 tatagatatg aaagcattga acaatacagg cgcttatggc gataatggac cttcagtttg
     5581 ctctgaatca ggtcataatg tacttccaac atataataat gtacctgcaa taaaatttga
     5641 tgggaaaact gtatatacta atttagttcc aggaggagca ttaagaggat atggagcaac
     5701 acaaggtaca tttgcacttg attgtgcaat tgatgaatta gcgcataaac ttaatatgga
     5761 cccaacagaa ataaagttaa agaatattat aaaaaaacaa actgaaggcg gaataatgaa
     5821 ttttccaata agaagttgtg cacttgatga atgtataatt agaggtaaaa aacttattgg
     5881 atgggatgaa aagtaccctg ttagggatat tggaaacaat aaggtgaggg ctgttggaat
     5941 gtctgtggga actcatggtt ctggtatagc caacatagac atggcaatag taaatatgag
     6001 gatggatgaa gatggaactt ataagttgtt ttctgggtct tcagacttag gaactggttc
     6061 tgacacaata cttgcacaaa tagctgcaaa agcattgaat acatctattg atagaatttc
     6121 cgtttataca ggagatacag atatgtgtcc atatgatact ggtgcatacg cttcttgtac
     6181 tacttatgta actggaaatg ctacaataaa atctgctgaa agtttaaaac aaaagatatt
     6241 aaaagtagca cagaagaaac tcgatttacc aatagatgat ttaatactat atgaagacag
     6301 agtttgttat agtgaagatg tagagaagtt tgttttgtta agtgaattag gaaaagaatc
     6361 tgttggtggt ggaaatcaag aagttttata ttcatctgaa tcttatggta tacaagagag
     6421 tgctaaacca ttcttagcag gatttgctga aatagagtta gataaaacaa caggggagtt
     6481 taaagtaata aattatgctt gttctacaga ttgtggtaca gttatgaatc ctgcacttgc
     6541 tagaatacaa gttgaaggtg gtgtaactca aggtataggg cttgcaatgt atgaagaccc
     6601 tagacatggt tctgatggaa agcttcaaac ttctaatcta ttacaataca aagttccaac
     6661 aaaaaaagat ataggaaaca taattgttga ttttgtagat aattatgagc caacaggtcc
     6721 atatggagct aaatctttag gagaaatagt tattcataca cctgcaccag caatagcaaa
     6781 tgcaatatac aatgccacag gagttcgtat aagagaactt cctataactt ctgagaaagt
     6841 ttatatggcc atgaaaaaat taaatcaggg aggtaaataa atgattgcta ttaaaagtaa
     6901 atacttaata tcttctgctg atgaaatata tacaaattct gctgttgtag tagaaaatga
     6961 cattataaaa gatatattgc caaatgaaga agttgaaata aaatataaaa atattgaaga
     7021 aataatagat aaatcagatg ctataataat gccaggattc gtcaatggtc atatgcatca
     7081 atatggagtt ctttctaggg gaataccggc aaatgtacat tttactgact ttgaaggttt
     7141 tttaaatgac tattggtggc catttataga aaatagaatt ggattaaaag aagtgaaggc
     7201 aacaactaaa gcatcagcaa tagaacttat agaatctggt gtggtagctt tttgtgatac
     7261 cctagaagct ccaaatacag aagaaggaac tttaattgag caaggaaaaa tactagaaga
     7321 aataggcatg aaagcgattt tatccttaga aagttgtgaa agaataagct ttgaaaatgg
     7381 acttagatgt ttagatgaaa attctaattt aataaagtgg agtagagaaa atagtaaatt
     7441 aataaatgga ataatgtgta cacatacaag cttcacatgt tctgatagat ttattaagaa
     7501 agctaaagaa gatgcaaaaa aattaaatgc accaatgcaa tttcatttat gtgagagtat
     7561 atatgagcca aactatgctg aaaaacactt tggcaaaaaa gcagttgact actataatga
     7621 tttagatata ttggatgaaa ctgtattagc atctcagtgt gtaaaagtaa atgatgaaga
     7681 aatagatata ttaaaagaaa aaggcgtaaa agtggttcat atgcctctta gtaactgtga
     7741 agtaggtgga ggtttttctc cagtaccaaa aatgattaaa aaaggcataa aagtggcact
     7801 gggtactgat ggatatataa atgatttttt tgaagttatg agaggtgcat ttttaatgca
     7861 taaatcagtt gaagaagatg catcagtaat gccagcaaat ttagtattta gaatggcaac
     7921 agagcatggt gcttatgtat taggacttca aaattctggt aaaatagcag ttggtaataa
     7981 ggctgatata atagttatgg aagatgaatt taaaactcct gtgactcttg ataatatatt
     8041 tgaccagata gtagttcagg gtaaaaaaga atttatatca aatgtataca tagatggtag
     8101 acatatttta aaagaaaaac agttagttga tttagataaa aaagctattg ttaaagaaat
     8161 gaaagaagtt gcttgtgaat tttggaaatt ttaacgaatg taaatatata tgaaaagggg
     8221 aagcagataa tgtataattt tttaggtaaa gaattaaaat caccacttat aataggttca
     8281 ggaccattaa catatagtgc agctggatgc aagatattaa gtgatgcagg agcaggggca
     8341 gttgtgacta agactataag aaaagaaaga gctataaatc cagcaccaca tatggttaga
     8401 aatacagcta atgctctttt aaataatgaa aaatggacag attttgagcc agagcaatgg
     8461 atagattttg aaataccaca aatgaaaaga gatgggactg tctgtatagc ttcaataggt
     8521 catacaattg aagaaagtag cgaattagta gagaaagttg caaatgcagg agctgacttt
     8581 attgaattag tatcttatga ctatagagat ttgataccaa tgcttaaaga tgcaaaggaa
     8641 agagtaaata tacctgtaat agttaaactt cctcctatga tagatgagat aggcgatttt
     8701 gctaaaaaat tagaagaagc aggagcagat gcaataactg cttgtgactc tgtaggacca
     8761 gcatttagaa tagacataga aacagggcaa cctttattag gtggaaatgg aataggatat
     8821 ttaagtggag aaactatcaa gccaataact ttacagagaa tatatgaaat aaaaaaacaa
     8881 gttaatatac caataattgg cttaggtgga tgtgtaagtg gagatgatgc cttagaaatg
     8941 attatggcag gtgctgactt tgttggtata tgttcagtag taattttaaa aggtgcacaa
     9001 gtaatatcta aaatacatga tgatttaaaa tctaatctta atagattagg atataatact
     9061 atagaaaatg cttgtggtat agtacataat catatgggag atgttaatga aagacttagc
     9121 tttgagttta cttatgatga agaaaaatgt actaaatgta agatgtgcga gagagtttgt
     9181 gcatatcaag cgagaaaatt gaatgataag atggaattga atgaagaaga gtgtagatat
     9241 tgtggacttt gtatatcagt ttgtaaacca aaagcacttg gaagaaaaat ttcatgttca
     9301 gagctgaatg tatagattat tttataaaaa acaaagtttt aaataaaaat aagaaaggct
     9361 atctcaaaga gataagcctt ttttgttgtt gaccttattt ttgtttattt gtagttattt
     9421 tagatattaa ttagttttga agtaataaag tgaatcaatt ttgtttaatt aaaattttaa
     9481 taattctgat atatatgtta acattgtata gcatttgtgt aaaatgttat aataattaat
     9541 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
     9601 tgtagttagc ataaaaaatg atatgtcgga agtaaaacaa gaaataaatg tgttaaaaga
     9661 agatatgtcg gaagtaaaac aagaaatgac tgtaatgaaa gaagacatgt cggaagtaaa
     9721 acaggaaata aatgtattaa aagaagatat gtcggaaatg aaacaagaaa tgactgtaat
     9781 gaaagaagat atgtcggaag taaaacagga aataaatgta ttaaaaggag atatgtcaga
     9841 agtaaaacag gaaatgattg taatgaaaga agatatgtcc gaggtaagac aagagataaa
     9901 tataatgaaa gaggatatgt ccgaggtaag acaagagata aatacaatga aagaggatat
     9961 gtcggaagta agacaagaaa tgactgtaat gaaagaagat atgtcggaag taaaacagga
    10021 gataaatgta ttaaaagaag atatgtccga agtaagacaa gagataaata taatgaaaga
    10081 agatatgtcc gaagtaagac aagaaatgac cgtaatgaaa gaagatacat cagaggtaaa
    10141 acaagagata aatgtaatga aaaaagacat gtcagaagtg agacaagaaa taactgtaat
    10201 gaaagaagat gtttccgaag ttaaatcaga agtaaacttt atgcaaaata aaataaataa
    10261 cattaatgaa gatatgaatg gaattaagga cgaggtatcc atagcaaatg aaaaattata
    10321 tggaattgaa attaagattg atagtctaga atgtgaagat aaatcaatga aagagataca
    10381 gatagaacag aataatattt taggaagttt attacataat tctgaaataa ataaagctac
    10441 tcatgataat atagaacata acatagcata tataaaaggt gacactaatt ctataaaaga
    10501 ggatatagct gaaataagaa gagacttaaa tttagtagag ttagccacta gtaaaaattg
    10561 gagtgatatt gtcaaactaa aatcagtaaa gtagtatttt aattcactaa tttatcatcc
    10621 acaaactaag aattttatat aaaagaattt atagtgttct tactatttat tataaatttt
    10681 agaaatgaaa ataaaaaatt
//
LOCUS       DAYAOY010000126        10406 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYAOY010000126 DAYAOY010000000
VERSION     DAYAOY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.126"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(332..1582)
                     /locus_tag="ACGI6B_003508"
     CDS             complement(332..1582)
                     /locus_tag="ACGI6B_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HFG9752917.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1614..2555)
                     /locus_tag="ACGI6B_003509"
     CDS             complement(1614..2555)
                     /locus_tag="ACGI6B_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFG9752918.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2795..3871)
                     /locus_tag="ACGI6B_003510"
     CDS             complement(2795..3871)
                     /locus_tag="ACGI6B_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFG9752919.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4244..4759)
                     /locus_tag="ACGI6B_003511"
     CDS             complement(4244..4759)
                     /locus_tag="ACGI6B_003511"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFG9752920.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5122..6192)
                     /gene="leuB"
                     /locus_tag="ACGI6B_003512"
     CDS             complement(5122..6192)
                     /gene="leuB"
                     /locus_tag="ACGI6B_003512"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFG9752921.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6199..6690)
                     /gene="leuD"
                     /locus_tag="ACGI6B_003513"
     CDS             complement(6199..6690)
                     /gene="leuD"
                     /locus_tag="ACGI6B_003513"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFG9752922.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6705..7982)
                     /gene="leuC"
                     /locus_tag="ACGI6B_003514"
     CDS             complement(6705..7982)
                     /gene="leuC"
                     /locus_tag="ACGI6B_003514"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFG9752923.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7939..9681)
                     /gene="leuA"
                     /locus_tag="ACGI6B_003515"
     CDS             complement(7939..9681)
                     /gene="leuA"
                     /locus_tag="ACGI6B_003515"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFG9752924.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9805..10051)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10199..>10406)
                     /locus_tag="ACGI6B_003516"
     CDS             complement(10199..>10406)
                     /locus_tag="ACGI6B_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752925.1"
                     /translation="NNQCIFSIKSKQSQKFNYKIIEKIEVVKNILNIYTDESNYKIRL
                     RALSLEDEKKLLNIIDEKMKKFKA"
BASE COUNT         3159 a         2001 c         1065 g         4181 t
ORIGIN      
        1 ataaatataa taattatatt tataaaaata aaatataaat ttcaaaatca ctttttttat
       61 taactttttt ataattataa taaaaatata taacagtact attataaata cacatttaaa
      121 accttataaa tatacaaata caattatatg aattttcatc ctcaattata gcatagaata
      181 tgactatcta tatcctaaaa ttatccattt ctaaaaaaaa ggtaactatt attaagaata
      241 tcaacttata gaatttatat tccacatacg attttcaatc aaaaaaacaa gggctaaaaa
      301 gtgaattgtg cactttttag ccctttatta attattcatt tatttcatgt aagaatagac
      361 cacatcttac tttaggttca aaccatgttg acttagctgg cattatctta tttgcatctg
      421 ctactcttat taaatcttca atactagtag gatacattgc aaatgctact ttcatatctt
      481 cattaactct tttttctaat tcttcaagac ctctaattcc accgataaaa tcaattcttt
      541 tatctactct aggattatca attcctaaaa tagcatcaat aacatagtct tgtaatatag
      601 aaatgtctaa tgagtcaaca gggtcttcta tttctaataa atttttattt gctttcattt
      661 tataccactt atcacctagg tacattccga aagttccttt tttgtcaggt ttatattttt
      721 cttttccttc tatttcttct agttcaaaat tttcttttat tttatttata aattcttcct
      781 cgcttaaacc atttaagtct ttgataaccc tattataatc caaaaccatt aaattctcat
      841 ctggtgcaat cattgctatg aagtaattaa attcttcttt tccagtataa tttggatttt
      901 tagctctcat ttctttggct acattttctg ctgaagctgt cctatgatga ccatctgcaa
      961 tgtatagata atctaatttt tcaaatttat ctactaggtt atctattata tcatcattat
     1021 caatcttcca tactgtatgc tctatcttgt catctgttat gaaatcatat aaaggttcat
     1081 tattatccat aaaatcattg attatatcat ctatcaccct ttgatgtttg taagttacaa
     1141 gtatagttcc cgtattagca tcacagtatt ttatgtgttt agttctatca atttcctttt
     1201 ctggtcgagt gtactcatgt ttttttattg ttccattcaa gctttcatca actgatatac
     1261 atgctactat accaacttga gctctaccat ccatgatttg tttatatata tatatacact
     1321 ctttgtcatc ttttactaga atttcttgtt ttcgaaactc atctaaattt tctcgcgctt
     1381 ttaaataaac tttttcatca tataaatcta tattttcatc taaatcaatt tctgatttat
     1441 ctatatgcaa aaatgaatat ggattccctt ctgcaattct tctagcttct ttactattca
     1501 taacatcgta aggaagtgct gctactttat ctactatata tttatttggt ctaatcgcct
     1561 taaatggttt aatcgttgcc attactaatt tcctcctaat tatacaaaat attttataat
     1621 gctaccccta ttagattgtt gaagtcaaga aagaaatctt gaatcacatt tactatttcg
     1681 tctcctattc tttcttgagc ttctactgta gatgccccaa tatgaggtgt aggagaaact
     1741 cttggatgat ttactaagtc taaatttaat gtaggttctt gttcataaac atctatagca
     1801 gctgctgata atttaccttc atctaatgct tttagtaaat ctttttcatc aactaaaccg
     1861 cctcttgcac agtttattaa ataagcccct ttcttcatct tatttatttc tttttcagtt
     1921 attactgccc ccttattctt atcaaatggt atgtgtaaag ataagaaatc tgcattttct
     1981 aaaacttctt ccatatcaca gaatttataa ttgttaaagc cttgcgcctc acccattatg
     2041 tcagtatata taacattcat acctaataat tctgctctct ttgcaacttc ttttgcaatt
     2101 cttccaaaac ctataagtcc aagtgtcttt ccattaattt ctgtaccttt atactttttc
     2161 ttctcccatt ttccatctct catagttaca tttgcagtat ttatgaatct tgcaagaaca
     2221 aataattgac ctattgtaag ctctgcaaca gatatactgc ttgcatttgg agtattcata
     2281 acttttatgc cattagcttg agcatattta acatctatat tatccagacc tactccacct
     2341 cttactatca acttcaactt tttaccctct aatgctttat ctattacatc tttagtaact
     2401 ttagtagctg aacgtactac aagtacatca acatctttaa gtttatctcc taaaacatct
     2461 ttttcataaa actgctctat cacattaaaa tctaactctc tcaattttct cgctgcttct
     2521 ttctctattc catcagtaac taatatatta tacataacaa actcctccaa tttttattca
     2581 cttaattttt ccaattttta ttttttaaag tattttcatc aataaaaaac tttacttaat
     2641 ttaactttct gtttatataa ttttcaaaat atttttttat taaagtcata tcaaattttt
     2701 ctattgtttg ttaaaaattt aatatgtaat ttttataaaa tattatttat tatatattct
     2761 aaatttatct taaaacatac atatttttac aatattattt attaaaacct aatatatcat
     2821 caatattatc taataattct tgtacatctt cgacagtata gtcacccata tgtgaaattc
     2881 taaaagtttt ttcttttaaa tctccatagc catttgctat ttggaatcct cgttcttgta
     2941 attttgaatt taaatttgat acactaatac cttgagtatt ttctataaca gtaagtgtat
     3001 tggataagtg attttcattg gtaaaaattt ggaagtgttt tttagcccag tttctaacta
     3061 agtttgccat gtcttcatgt ctgttgaatc tgttatctag gccttcatct aatatattat
     3121 ctaattggaa atctaaagcg aacatgtgag aaagagatgg tgttgaaggg tattggtagt
     3181 ttttcttgat taagtattca tacattgcta acaaatcaag atatactcct ctaaaaggta
     3241 cttgcttagc tctatcgatt gctttttgag aaaatgtgca aatagacatt cctggtggta
     3301 atcccagtgc tttttgtact gatgttatac aaatatctat tccaatctta tcaacctcga
     3361 cttttattcc accggctgaa cttacagtat ctacacagta gattacatcc tcatactttt
     3421 taacaacttc tccaatttct tctattggat ttcttattcc agttgaagtt tcattgtgag
     3481 ttacagttat taaatcgtat tctccggtag aaagcacctt atcaaccatt tctggcgttg
     3541 ttgcctctcc taattcaact ttaaatatat ccgctgggac attattagcc actgccattt
     3601 tgtaccatct atcaccaaag gatccacagg aaaatactgc tgcctttttg gatgtacagg
     3661 aacgaatgga gccttccatt aaaccagttc cggatgacgt ggataaaagt atcgtgttgt
     3721 ttgtatagaa tagtttttgc atcttttcac ttatactctt ttggagcata gatgcatctt
     3781 ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa gacttcttct ttaacatcaa
     3841 taggaccagg aataaataat ttcttactca ttataattcc ccccttttta gtttgtaata
     3901 tggatagcgt ttttactgtc cttattttta ataactataa tatcaagctg aattaactat
     3961 gtcaacataa aatttatttt ttatatataa tttattttat atattttatg aaaacattca
     4021 ttttctacct tttcataagt ctgaattttc tgaattattc cctcaaaaaa aatatacttt
     4081 ttatctatat aaattaattt ttttagtatg taaattttac gctattacga taaatctttt
     4141 tagtataact aatttatttt tatcttgtat ttttattgta tttttgaatt ttattttttc
     4201 taaatataaa atttatgttt taaaatataa aatttttatt taatcaaagt tcaatatatt
     4261 ccactttttc tttcgattct tcaaaaaacc attcttcatt agaaaaatca ccttctgcaa
     4321 tttgtccaat aggcattttc atatactttc cagttgctgt cactgctata tctccatttg
     4381 gtagaattat ttctcctgta ccttcaaata attttctgtt ttctttagtt attcttccaa
     4441 caacttttat agtagcatct gttggtactg gtttcttata tttcaactct aaagatactg
     4501 ttactcccca tatttggtca tcatttatac ttacagctct tccaatagtc tcatccaata
     4561 ttgcagcaga catacctcca tgaactctcc caggataact ttgatgccaa tcctttgtat
     4621 tacaaattga tacaagctct ccattttcaa gttcataaaa atctgcttta agtcctagct
     4681 cattttgtgt tccacaaact aaacaagatt tactactatt ttgcttcttc aaaactttat
     4741 actttgccaa aaaatccatc tcctaaccga cattagtagt cgcttaatat taaataattc
     4801 taaaatatat tatataatat taataaaatc cctgcaaact tctaataata aaaataggct
     4861 acttcaatat gataagttta atttaaagta ttattttcca aaaaataact ttatcaaaat
     4921 agaaactcaa agtagatata aacctctttt tcgttattaa aatagtaaaa atatatttga
     4981 cttaatataa aaaagagtgt ctcattaact aataaagttt attttgagac acccctcata
     5041 aaacaaatta tactacaata gatttttact aattctacaa gttatatatt taaattatat
     5101 tattcaaatt aaactataat tctaaatttc atttactatt aattcgccca tttcccttgt
     5161 tcctacaaca tttcccacac cattatagat atcaatagtt ctataacctt tattgagtac
     5221 actttgtaca gcatcttcaa tacacttagc ttcttcttct aagttaaaac tatgtcttaa
     5281 catcatgctt acagatagaa tagtcgcaat tggatttaca atatcttttc cagctatatc
     5341 aggtgcactt ccatgtattg gttcatacat accaaaacta tcttctctaa gtgatgctga
     5401 tggtaacata cctatagaac ccgtaatcat agatgcttca tcagatatta tatctccaaa
     5461 tatattatta gtcactatta catcaaattg gctagggtct tttacaactt gcatagcagc
     5521 attatcaaca tacatatgtg acagttctac atcttcaaac tctttagcta agtcttccat
     5581 tatacttctc caaagtcttg aactctcaag cacatttgcc ttatctacac ttataagttt
     5641 tttatttcta tttctggcta tttcaaaagc tctcttacca attcttctta tttcattttc
     5701 gttatatatt tctacatcat aagcattttc gataccatca atgatttttc ttccacgttc
     5761 tccaaaataa atgccccctg ttaattccct tacaacaaca aaatcaatgc ctttctcaac
     5821 tatatccgtt ctaagtggtg aagcttcttt tatactttca tacattgtag ctggtcttaa
     5881 atttgcaaat aaacccaatg actctcttaa ttttaaaaga ccagactctg gtcttttatc
     5941 tcccttacat ttatcccact ttggtcctcc aacagcacca agcaatacag cttcgttttt
     6001 tcgacaagtt tcaacagttt tattaggtag aggttctcca gtttcatcaa tcgcgcatcc
     6061 tccagctaat acatattcac aatcgaatct atgattaaat ttacaacaaa ttttatttag
     6121 aacctttata ccttcatcta taatttccgg tccaacacca tcacctttta tgactgctat
     6181 attacaattc atacttcact accccctatt tgcttttatt gagttaatta agccttcatt
     6241 taatataata ttttgcataa actcaggaaa tggttctgct ttatattctt ttccttttgt
     6301 tatatttttt atcacacctg tagaaaaatc tacttctata ttatcccctt catctatgtt
     6361 attggcagct tcttcacatt caagtatagg aagccctata tttatagagt ttctaaaaaa
     6421 tattctagca aaagtagaag caatcacaca ggatactcca ctttctttta ttacaattgg
     6481 tgcatgttct cttgatgaac cacaaccaaa gtttttagtt gcaactataa tatctccttt
     6541 ttttacctta gaaataaatt tatcatcaat atcctccata caatgcgttg ctagttcttt
     6601 ataatcagct atatttaaat atctagctgg tattattaca tctgtatcta tattgtcacc
     6661 gaatttaaaa acacttccat tggcaatcat aaattttacc tcccttaaat tatttctgat
     6721 ggttttgata tttttccagt tatagctgat gctgctgcta cagctgggct tgcaagatac
     6781 acctctgaat caacatgccc cattcttcct acgaaattcc tatttgttgt tgaaatggat
     6841 ttttctcctg ctgcaagtat acccatatgt ccccctagac atggaccaca agttggtgta
     6901 gaaactgctg ctcctgcctc tataaaagtg gtaatatacc ctttttctat agcttcaaga
     6961 tatacttttt gagttccagg aaatatcata actctaactc catctgctac ttttttgcct
     7021 ttcattatct cagctactac ttcaaggtct gatattcttc catttgtaca agagcctatt
     7081 acaacttggt ctatctttat atccttatct atctcatcta ctgttctagt attttcagga
     7141 agatgaggaa aggctactgt ttcttttagt tcagctaaat ctatttcgta tacttcatca
     7201 tagactgcat cttcatctgc ttcatatata tttacatctt ttgtcatagt tgaacactta
     7261 tgtgaattta tatattccat agtcttatcg tctactggaa aaattccatt ttttgctcct
     7321 gcttctattg ccatattaca aatagtaaat ctatcatcca tagataagta ttctactcca
     7381 tctccacaaa attccattga cttgtaaagt gctccatcaa cacctatctc ccctattata
     7441 tgaagtatta tatctttacc acttatccat ttatttggtt tattcttcaa aacaaatttt
     7501 attgctccag gaaccttaaa ccaacatttt cctgtagcca tacctgctcc catatcagtt
     7561 gaacctacac cagtagaaaa tgctcctaat gctccatatg tgcaagtatg agagtcagca
     7621 cctataatta catctccagc agttactata cctttttcag gaagaagaac atgctctatt
     7681 cccatatctc ctacatcaaa aaaatttaca actccttttg attttgcgaa acttcttgta
     7741 aatttacatt gttctgcact ctttatatct ttattaggtg taaagtggtc taaaaccatc
     7801 actactttag tcttatcata aacatcttca attcctaatt tttcaaattc tctaatggct
     7861 actggtgatg ttatatcatt acctaaaact acatctaaat ctgcttcgat taattgccct
     7921 ttcttaactt catttaattt agcatgtttg gctaatattt tctgcgtcat agtcatcccc
     7981 attttttatt tcctcctcaa tataactttt atttattgca ttcataagtg cattaatagc
     8041 tgctgtattt atgttttccg atatacttac accaaaatag acttcattat tgtgctctat
     8101 tcctacataa gctacccctc ttgaatgtga cccttcctct aatgcatgtt cccagtaata
     8161 tctaaattta taatctttca tgccacattg ctttagtgca ttattaaatg aatctacagg
     8221 tccatttcct ataccaacaa tcctttgttc atttcccata taacttattc ttgcagtcat
     8281 ctcaatcatg ttagtatcat tagaatcatc attttcatag tttaattcat ccattgattt
     8341 tattttatac ttcttaactt tataagggct ctcaacttca atatattctt tcttaaacaa
     8401 attaaatatt gcttcactac taagttcttc cccaattcta tcagattcca ttttgactac
     8461 atttccaaaa tcagaatgca tattcttagg tatcataaat ccataatctg tttccattat
     8521 ataaacagct cctccttttc cagattggct atttatcctt ataatttctt tgtattctct
     8581 tccaatatca tgagggtcta ttggtaaata tggaacttcc cagtaatcat tatctctgtt
     8641 tttcattgct atcatgcctt ttctaatagc atcttgatgt gaacctgaaa aagcacaatg
     8701 tacaagatta cctgcatatg gatgtctatc atgtaccata agttttgtac aatcttcata
     8761 tatacctatt atcttatcta tattgctaaa atctagctct gggtctattc cttgtgtata
     8821 aagattaagt cccacattta ctatatccat attcccagtt ctttcaccat ttccaaatag
     8881 tgttccttca agtctatcgg caccagccaa taatccaagt tcacttgcag ccgtacaagt
     8941 tcctctatca ttgtgagtat gcaaacttag tataatagaa tccctacaac ttatattttt
     9001 acaaaaccat tctatttggt ctgcatatac gtttggtgtt gccatttcaa ctgttgatgg
     9061 aagatttatt attacttttt tttgagtagt tggtttccaa acatctatca ctgcctcaca
     9121 tatttcaagt gcataatcca tctctgttcc tgtaaaactc tctggtgaat attcaaaagt
     9181 aaattctgtt tctggatatt tttttacttc ttcattaaat aattttgctc cttcaactgc
     9241 tatatctatt atttcttgtt tactcatatt aaaaaccaca tctctttgaa gaacagatgt
     9301 agagttatat aaatgtataa ttgctttttt acatcccctt attgattcaa atgttttttc
     9361 tataagatga gccctagatt gtgtaagtac ttgtatagtt acatcatctg gaatcatatt
     9421 ctcatctatt aattttctta aaaatgtata ctctgtattt gatgctgatg gaaatcctac
     9481 ctctatttct ttaaatcctg tatcaataag cattttgaac attctgactt tttcatttac
     9541 actcataggt gttggcagtg actgatttcc gtctcttaaa tcgacactac accaaattgg
     9601 agccttaaat atttcattat ctggccattt acgatttact atttgcattt tatcatattt
     9661 tttatacttt ccacacttca tgttatttcc ccctttttct gttttttgta ttcagtatac
     9721 ctcatatcga cgcaatcctg cacaattcat aaggataaat gtcaatttta tcattagaat
     9781 aaaaaaaacc tcatctctaa taaaattaga gacgaggttt tgctcgcggt accactctat
     9841 ttagctttct aaaaagctcc cttagtaata gccaatacaa taattataat gactatattt
     9901 cctataacgt ggaattactc cgattattct aagatttaaa tcttagaaaa cagctcctag
     9961 acaagttcag aactatcaac tactaaatca caccatcctt tagctctctg aaagtttcat
    10021 tgttcttact acttctattc atcacttttt taatcattta aattttctaa atattttgat
    10081 ttaatagtta ttatatacac attaaaatgt attgtcaaca caaaaaataa ttatctcaat
    10141 tttttatttt tgataagcaa ttctataaaa catgctaact attgaaaaca gtatgatatt
    10201 atgccttaaa ctttttcatc ttttcatcta tgatattcaa taattttttt tcatcttcca
    10261 aactcaatgc ccttaatcta attttataat tagattcgtc agtgtatata tttaatatat
    10321 tttttactac ttctattttt tctattatct tataattaaa cttttgactt tgtttagatt
    10381 ttatactgaa tatacattga ttattt
//
LOCUS       DAYAOY010000127        10352 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYAOY010000127 DAYAOY010000000
VERSION     DAYAOY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.127"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(120..719)
                     /locus_tag="ACGI6B_003517"
     CDS             complement(120..719)
                     /locus_tag="ACGI6B_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HFG9752926.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(737..3856)
                     /locus_tag="ACGI6B_003518"
     CDS             complement(737..3856)
                     /locus_tag="ACGI6B_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFG9752927.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            4077..4901
                     /gene="pdaA"
                     /locus_tag="ACGI6B_003519"
     CDS             4077..4901
                     /gene="pdaA"
                     /locus_tag="ACGI6B_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFG9752928.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(4998..5783)
                     /locus_tag="ACGI6B_003520"
     CDS             complement(4998..5783)
                     /locus_tag="ACGI6B_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9752929.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5883..6017)
                     /locus_tag="ACGI6B_003521"
     CDS             complement(5883..6017)
                     /locus_tag="ACGI6B_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752930.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            6248..6613
                     /locus_tag="ACGI6B_003522"
     CDS             6248..6613
                     /locus_tag="ACGI6B_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9752931.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            6691..7245
                     /locus_tag="ACGI6B_003523"
     CDS             6691..7245
                     /locus_tag="ACGI6B_003523"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFG9752932.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            7465..8139
                     /locus_tag="ACGI6B_003524"
     CDS             7465..8139
                     /locus_tag="ACGI6B_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFG9752933.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      8494..8590
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8644..8820
                     /locus_tag="ACGI6B_003525"
     CDS             8644..8820
                     /locus_tag="ACGI6B_003525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752934.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(8991..9230)
                     /locus_tag="ACGI6B_003526"
     CDS             complement(8991..9230)
                     /locus_tag="ACGI6B_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752935.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(9345..10184)
                     /locus_tag="ACGI6B_003527"
     CDS             complement(9345..10184)
                     /locus_tag="ACGI6B_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9752936.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
BASE COUNT         3595 a         1586 c         1026 g         4145 t
ORIGIN      
        1 tttttcataa tacaatctgc ttttttaatc aaaatttatt taattacaag ttgtttaatt
       61 ataaagtagg aatttagata tactcttata ccatgtaatt atgtataaat atttttttat
      121 tatacaccaa tagctttttt agctaattta tcagcttcct cattatactt atttccagaa
      181 tgagctttaa cctttgtaaa ttttacatta agcttattct taatactgtc atagaaattt
      241 ttatatgcta tagttccttc tttatttgtt ttccaaactc cagtacacca cttttcaatc
      301 ccttcatagt caaagtataa aatcaagttt tgaacattat tgtcaataca gtactgcatt
      361 gcaattttag aagcctctat ctctccagct acatttctca tacttactaa agttttctct
      421 tttccttttt cactataagt ttttataact tcattatttt ttaaaatcac aacacccgaa
      481 ccatacattt ttactgaatg ttcataactt ccatctacat atgcttctat aaactcctta
      541 tcttcttgaa aactcaattt ttctgaccca tcaatatatt ctttagcttc ttgaaaagta
      601 gaaaaactct tatattcagc tcctgagaat ccatttactt gttttttgca ctcatcccat
      661 gtattatata cacctatatt tttacctttt ttcacagcat aaaacttttg ttttgccaaa
      721 tatatcactc ccactcttaa tttttatctt cttctctcat aaatatatct aaatttataa
      781 gatagtttct atcaaaatat tttactttca tatctttttt tataatatca atatggtaca
      841 cttctccttt tacagctctt tgtcttatat tttcatttat atcttttttt ataatatcct
      901 tatcaacatc attgttatcc attttagcat caacattcaa cgcttttata gtatcaaata
      961 taacagattt tagtattaaa cttctagatt tatttaaatg tcgccttata taagtataaa
     1021 actcattgct attaaccttt gaaattttac aatcgtgtaa atctttctct aatctcttta
     1081 acacacttat attagttcct acatcaaaaa tcacaaaaca tccatagtca tatttgcttc
     1141 ttaaaattct ccccatagct tgctttacct ttatggtcat ttgaggataa tttatagtat
     1201 agtagtctat accatacttc ttcattatag taaaatacaa agggtcttta ggatttagat
     1261 tcggaagctt gtctaaagtt acacaaacca aaccatcccc tggaatgtct acgccttcaa
     1321 aacatccttt tgagccaagt actacacatt ttctatttaa atcgttaagg tgtctaatgc
     1381 cctttttatc agcataaatt tctaagttaa aactatgtaa gtattttttc aatatttcat
     1441 aagttttttc ttgtctatct ttactattaa aaagagctaa tgtatgacct tctgtaatct
     1501 ttgatatatt agatataatt ttactcatct catttggaaa ttcagaattt ctgtactcac
     1561 aaatatcaga aaatccaact acagagactc tatttttata atcaaaaatt ggttctataa
     1621 tcttttccac attcttaact ctgtctattc ctaatgtatt tttaaaataa gacatacttt
     1681 cagataaact caaagttgct gataaaaata ttcctttttc taattgagat aaaatatttt
     1741 cttcaaataa atctgctatt tttaatggta ctactcgaaa ttcaaagtca ttataattct
     1801 tatcaatttc aactatcctt gcataatcat ccttttcatc atactctaaa aagatttcaa
     1861 atataatctt tatatcctct aaatctcttg tctttgcctt tccaaattta tatatatctg
     1921 cttctttatc tatactatca tcatcaatat tcctataaat tattattatt attgaaacta
     1981 aattttttat tattttctca caactcaact ttatcttttc actataggct ctataactta
     2041 tttcagtatc tataccatct ttttttagtt ttcccacaat ttcatctatt tgcaaattca
     2101 gttcccatct taaattataa ttacttacat tattatattc attacaatta ccaaacttta
     2161 atattgaatc tatctcttca ataatgaaat ttatacttct agcaattttt tgtttatctt
     2221 ctcttccaat ttttaataca ttataaaatt tatcaaaagc ctttattttt ctcatatttc
     2281 tactatattt tttatatata aagctactat tttgaataaa ctcgtaagga taaatttctt
     2341 gtaataagta ccttaaagac tttgaattta taatacttga aaagaaatca tatccttttt
     2401 cagtcaaatt atgtgcttca tctactatta tattttctaa aggtttttca tccttatacg
     2461 gccatttagc taatagtgaa tgatttacta ctgttatatg ttcttctttt aattcttcta
     2521 ttctgttttt atataaacaa tcttttttgc attttttagg tctgcacata tttgggtcac
     2581 aacttacatt tcttaagtga gtacttattt ccttgaagtt gtcaaacact aaataattta
     2641 tttcttctat atctccatac ttgccacctt caactaatct cttcaaaaat attaaagata
     2701 aaatttctcc tttagtaggg ttttgagact cataatcatc tatatatgct tccagtctat
     2761 ctatacaaat atagttattt tttcccttta tatatccgta acttactttt ccatttagac
     2821 ccaaagaatc taacacattt ggaatatctt tatttattaa ttgtatctgc aattcctttg
     2881 tgtcagtaga aattaataat ctttttttat taattctagc ctctaatatt gcaggcaata
     2941 aatatcctac acttttacct ataccagttg gtgcctctat acatgcaatt ttttcttcat
     3001 cttcgctatt tctaaacaac tctcttattg ttttagtaag ctcatattga ccaggtctat
     3061 attcatatgt aaatccttct ttattctccc aaatagtttt gtattttaat aattcttcat
     3121 aatttttttc ttcttcattt aaaattttta aggtttctct ttccttcttc ttttcttctt
     3181 ttgagtcaaa tatattatat tcttttttta tatttatatt atttgataaa tcataatttg
     3241 cctcttctaa aaatttgctc cattcccatg ttggaagatt aaatttttta agatatgaat
     3301 ttattttaaa actcaatggc tccaaagtag ttttttcata gttattaaat tttactaaaa
     3361 ggcaatttac aatatttatt atatcaatag catctgataa agctctattt tccactttag
     3421 aattacaatt tgttaaagta ttttttaagt attctaagct ataatcttta tgatatggtt
     3481 ctaaaataac tgctaattca attaaatcaa taaatttatt ttttaacttt ggcatatgat
     3541 actcaaaaaa cttttttttc agacttaaat catgacatat aatgaaacta tcttctacaa
     3601 aatttacaag tctatcttcc accagttcta ttttattagc tatttctaaa tcatttttat
     3661 ctaaattttt acatactgag aatacttcta taggtacctc aaatttgttt tttattaatg
     3721 attcataggt atatattttc cagtctttaa ccttgacagc tccaacttct aaaatttctg
     3781 aatttaaaca atctaatcca ctaacttcta tatctaaaaa aacaacatca ttcaatacac
     3841 ttattatatt atccattttt atatcccttc aagttaaacc agcctaactt atttacaatc
     3901 atttctcttt atattttatc atttatatac actttattta agataattaa tatcaacttt
     3961 atatcttaca gtaaacttct ttcatttatt atttgtatat gtgaatattt ttaactataa
     4021 atattcaaac tatattataa gaaatatact ttaaatttta aataagaggt gaaattgtga
     4081 aaaaagatag cctaaaaaaa tacattatga taggagcatt tgctctaaca ctttttggaa
     4141 ttgctagtat aaattttaaa tcattagata aaacaaaaac tcaaatttct agtccaacac
     4201 tcgacacaca tgaatatgat tggtatttta accctaggga agatggtaaa cagccatcac
     4261 ctataaaaga agctgatttt tttaaaaaat acggagctta ttatgtagga aatcccaatg
     4321 aaaaagttat atatctatca tttgatgctg gatatgaaag tggaaacact ccaaaattat
     4381 tagatacgct taaaaaacac aatgctaaag ctcaattttt cgtagttgaa agttatataa
     4441 aaagcaatcc agagttaata aagcgtatgg aaaaagaagg acatttagtt tgtaatcatt
     4501 caaaaagcca tccatcaatg gcaggtatta cagattttga aaaattcaaa gaagaaatta
     4561 caagtgtaga aaaagcatac aaagatgtta ctggaaaaga aatgcctaag tattttagac
     4621 ctccaatggg taaatttagc gaacaatctt taaaatatac acaagattta ggatataaat
     4681 caatattttg gagttttgct tatgtagatt ggtatgaaaa gaaacaacct actcatgaat
     4741 ttgccaaaaa caaaatttat tcaagaactc atcctggtgc aatagtttta ctgcatccaa
     4801 attcatcaac aaatactgag atattagatg aggttcttac acattgggaa aaagaaggtt
     4861 ataaacttaa aacacttgat tatttaaata ataaaaaata gtatcataaa tttaaaaaaa
     4921 ctctatcatg aattatagta aaaatatagt tatttataaa tactttaatt ctcgatagag
     4981 ttttttattt tttatagcta tttaaaaaga attgtccatt catttatctc tttgtatcct
     5041 atattatgat atattttacc tgctacatca ttattataaa aaagacatgg aacttttcct
     5101 tctaacaaca attctttact caaattgtaa accatataac ttgcaagtcc ttttccccta
     5161 tatcttttat ctgtacttac agagaccacc attgccaaaa aactagtttc cataccagtt
     5221 gacacagtgc taatcatagt atcatcattt ctaataaaat atgctcttac atttccctct
     5281 tttaaatgaa cttttctttc ttctatgtaa tttttactta ctttatagtc tgacctattt
     5341 aaaagttttc ctatttcatc aatatctttt tcctgagcat tttctatttt atactccttc
     5401 atgttggaaa aatctatttc ttttatttct tttaatacac aaaatttatt atctagtttt
     5461 tcataaaaat ctttgtattt acttattagt ctatctatta cacatttttt tccacttatg
     5521 ctttctacat ctaaatcttt tatatgattc ttcatttctc ctatattaaa attattttta
     5581 aaactataaa ttataagtgt tgacttattt tttaatatta tagaggttat atttccacct
     5641 tctacctgag accatatatc ttgatattga gagctaaatc cataattctc aacgtatcca
     5701 attatgaata aatttaaact ttcttcatgt gatacatatt gccaaaattc tttttctaaa
     5761 gtattatcta tttttacaat caagcagata tcctccttct atgttaataa tgatatacct
     5821 tataatttat agtatcattt aaaatcatta tattctacca aatattctaa atttttaaat
     5881 atctatgcaa tatgttcgta tttattttta tattctgtta ctgcttcact ccaagcttct
     5941 tctactgaaa tacctctctt atttgcaatt ttttgtgcta attctattat tttcatagct
     6001 tttcttgata tattcatata tattcctcct ctaaattatc ataagttatt ttaaagttaa
     6061 atttctatag tcatgttata tatttattta cttatatctt actaattatt tatgtactaa
     6121 atatgaacta caatagaaga aatttagaac ttaataaaac ataaaattta tatacagttc
     6181 atatatattt gtcatatcac aagacaagtg atataattta ttatcatttt actgggggtg
     6241 atatgctatg aaaataagag ctgaatatac atgtcctctt gaaattgtac atgacataat
     6301 caaaggaaaa tggaaaacta tccttatatt tgaattaaga caatctaata aaacattttc
     6361 tgaattgcaa cataatattt atggaataaa tcaaaaaatg ttgattcaac aattaaaaga
     6421 gttacgtgat tttgggatta ttgataaaat aagttctgct ggatatccat tacatgttga
     6481 atattttctt actaatcgtg gtaaaaaaat gttaaaagca gtggaaataa tgcaagaaat
     6541 aggaatcgaa tatatgcttg aacatggtca acaagaaatt ctagataata aaggaatttg
     6601 ctacaataag taatcctata cataccaaaa agtaagtaat ttacattttt tacaatagta
     6661 ttataataaa aataaatagg aggtataatc atgtctatag ctttaatatt gattgatatt
     6721 caaaatgatt attttaaaaa tggaaaatgt gaattatttc aatcagaaga aactgctgaa
     6781 aatgctaaaa aaatactact gttttttaga aaaaacaatt taccagttat tcatatacag
     6841 catatatcaa cagataaaac agcttctttt tttcttccta atactcatgg ttcagaaata
     6901 aataaaattg tttttccact agataatgaa gaaattataa ttaaacacac accagatagt
     6961 ttctttgaaa cagatttgca aagtatcctt gagaaaaaaa atattactaa tctagtaatc
     7021 tgtggaatga tgagccatat gtgtattgat acaagtgtaa gaacagcaaa gaaacttcga
     7081 tacgacataa ctctaataag cgatgcatgt acaacgaaga atttatcttg gaataatata
     7141 gaaattaatg caaatactgt acatcaagta ttcatggcat cactacagaa cagttttgca
     7201 gatgtaaaaa acacagataa atttttatct aactattaca aataaaaaca tacactatgt
     7261 taaaagagca agtttgattt atttaagctt gctctttaat catttaaaat tagatgcttt
     7321 ctatcatttt tatatacata atatacgttt atacatttta tattcatata taactattat
     7381 ataaatttta aactttacta tattcacata aaaaatgtag tattatagta tatataatat
     7441 tatatagaag ggggtgctag ataaatgaac catcatatat tgattcaatt tgaatatata
     7501 gcaagaatca ttactgctgg tttctgtggg gctttaattg gatatgagag aaagaataga
     7561 ctaaaagaag ctggaatacg cactcatttt atagtggcat tagcttcagc acttataatc
     7621 attgtttcca agtatggatt taatgatata gtgggtactc caggtattgg tctagaccca
     7681 gcccgcgttg cagcacaagt tgtcagtggt ataggttttt taggtgctgg attaatattt
     7741 attagaaatc aatcagtaag tggtcttact acagctgctg gtatatgggc aacagccggt
     7801 acaggtcttg ccattggctc tggattatac ttagttggta ttgtttcaag tatcattatt
     7861 gttattgtac agataacact tcatagagat aggaaatgga tgaaactccc tacagcagag
     7921 caagtttctc ttaaaatttc taacagcact gattccatag aatatattga aggtaaattt
     7981 aaaaaatcta atattgaaat tataaacatg aatattaaac gccttgatgg ggattggtta
     8041 gaagtagtta tatatactaa gttacctaaa ggatataaaa aaacaaattt attaaaatta
     8101 tttagcgata atcctcttat agaaacttta gaaatataat ataaaaattg aagcttaaaa
     8161 ataataatat atttaatcaa ggtcaatata taaataatta agggatgagt ctatataaaa
     8221 ttcatccctt gttttttgcc attcatatat attttttcaa gatatttttg tttatctatt
     8281 atctagccaa gtaacccttg tgaactaaac tttttaatac aaaaacttca atacaaaact
     8341 aaatataatt tttattaaac attctataca tatagtttgt ttcttgagtg aaaaatagat
     8401 ttcatattgt taagatattg ttaaatattt caattttctg acaagtatgt tgaacaataa
     8461 cttatcctag tgtattattt tagttgtaga ataaaactaa atcggcaaaa ctagagaaat
     8521 ttagtgacgc aaagctatag ggactaacgc ttataaaata aactatgagt tatgtcagcc
     8581 agttgccaaa aagatattgt tctttttgtt ttatgaaagt tttttagggg gatagtaata
     8641 tatatgttta gagatgaaat ggataaatgt acacacatgt taactgctta tattagtagt
     8701 ttatatgatt attgtgattt tatagataca cagctagatg attttatact agagtacgga
     8761 gaaaatgtag tagaatcttg tttacatcaa gtgatggtat tggtaagtaa gtataattaa
     8821 atagaaattg ttttctagag aacccttttt agttttatct acatttaaaa atgttattat
     8881 attaaaaaac taatcttcca tatatgcaaa gttttcttcc atataacaat ttcttcccat
     8941 ttatactttt tcacttgtat ctttaataaa attcaacttc aaaaattatt ctatttctta
     9001 tctttacacc aattcctatc agtattatcc tttatatagg ttctaactat ttcgccacaa
     9061 tctaaacata aatctgaata tatagtagat tgtactggaa aagttttatt tataacttta
     9121 tctaattttc catctcctct atattttaat ccaatagttt cagcatatat gccaccagtt
     9181 gttaaaattc ccttttcaat atttacactt ccacaattag aacatttcac atttataacc
     9241 ccctaaacaa ttattttttc atgctatctt ataatttaaa atataatttg ctaccgattt
     9301 tataacacag tggcaaatcg gtgccaaatc aacatttttt cattctattt accatctaat
     9361 ccattgaaat atagatattt agttatagga acttgaattt ctgttacaaa ctcctctaca
     9421 attccagtct catgtatatc tatcttatag atttctatag ggtcagcaat tatcttataa
     9481 ttattttctt ctataaattt aaacaccttc tccatataca tcttattatt agaattactc
     9541 ccagtataat ttaaagttac ataatatcct tcatcaaaaa ctatattata tactttctca
     9601 ttactttcca gaaaacaaaa tacagattta aactcattaa atatgccttt attaattgct
     9661 tcagtagaaa aggcagaacc aatattgttg tttccaagta tattaaatct atcttcatat
     9721 tctttctgaa gtttttgaat taaaaaatcg aagtcttcat ccctctttat atcagcattt
     9781 aattctaaag cttttctttt ttctatatat acaacctcta tctcatctat ttttgtattt
     9841 tgaattacac tttcaattga actcaatctt tttataatat tttccttatg gcttataaat
     9901 tcagctattt ttttatcaat taaatcgagt tcttcattta aaatattttt cgtagactct
     9961 atacttctgt catctaggta ttcttttatt ttcttcattg gaaagttcaa gcttctaaat
    10021 tcttttatta aatttaattt ccatatatct gatatattat acattctata tccatttata
    10081 tctctaactg gtcttagaat gcctatttct tcataataca ttaatgaatc tctacctatt
    10141 ccatatattt tagatatctc tcctatttta tagtaatctt tcatttatat aataaactcc
    10201 tccgaaatct tatttttatt ttaaatcaac attattactc tataatacta tagtattaga
    10261 atatactaaa aaaatcttta aaacaaggat aggaaaataa acatcctaat aattaattat
    10321 taaaatattt ttatattgta atattaggta ta
//
LOCUS       DAYAOY010000128        10297 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAYAOY010000128 DAYAOY010000000
VERSION     DAYAOY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.128"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(81..1835)
                     /locus_tag="ACGI6B_003528"
     CDS             complement(81..1835)
                     /locus_tag="ACGI6B_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HFG9752937.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(2053..2943)
                     /locus_tag="ACGI6B_003529"
     CDS             complement(2053..2943)
                     /locus_tag="ACGI6B_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9752938.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            3092..3952
                     /locus_tag="ACGI6B_003530"
     CDS             3092..3952
                     /locus_tag="ACGI6B_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9752939.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            4408..4974
                     /locus_tag="ACGI6B_003531"
     CDS             4408..4974
                     /locus_tag="ACGI6B_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9752940.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            5014..5595
                     /locus_tag="ACGI6B_003532"
     CDS             5014..5595
                     /locus_tag="ACGI6B_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9752941.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5652..6569)
                     /locus_tag="ACGI6B_003533"
     CDS             complement(5652..6569)
                     /locus_tag="ACGI6B_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9752942.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            6699..8330
                     /locus_tag="ACGI6B_003534"
     CDS             6699..8330
                     /locus_tag="ACGI6B_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9752943.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            8333..9280
                     /locus_tag="ACGI6B_003535"
     CDS             8333..9280
                     /locus_tag="ACGI6B_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9752944.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(9565..9828)
                     /locus_tag="ACGI6B_003536"
     CDS             complement(9565..9828)
                     /locus_tag="ACGI6B_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HFG9752945.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(9927..10172)
                     /locus_tag="ACGI6B_003537"
     CDS             complement(9927..10172)
                     /locus_tag="ACGI6B_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752946.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         3599 a         1470 c         1421 g         3807 t
ORIGIN      
        1 ttttttattt aaagaatata tataaatatt ttaaaattct ataattttta aaactatata
       61 aactttattt tcatatgttt ttattctcca attgatatat tatttatgcc attattttta
      121 acttcaataa ttggttgtcc atttcctaaa atataatctt tagaccaacc ttctggcaat
      181 ttatttatcc catttccaat taacttcaag tgtaaatttg atggttcttt tttcagattt
      241 aatttaaata ttccatcaaa ggtaccttta ttatcgcttc caaataaact tatattgcca
      301 tttatattcg tagctgttac atttacaatt ccattgtttg ctctaaattt tatttcagat
      361 gatatagttt cagctgttac acctgaaatc ccactattag tatttatatt gaaaggattt
      421 gttacattgt tatcaacaat gttagcaatc ccgtcattga catcaacagt agctcctcct
      481 ttaatattat aaagttctac tgaaccactt tcaacatcta ctttaaaatt agaatacatt
      541 ttatcgggaa ctccaatttt tatcacattt accatcctat ctttatttgc atttatcata
      601 ttgtcgccat tcatatcttg caaatcacta tctttcttat ctattttttt cttagcactt
      661 acaactagct ttccattttt tatattatta gaagaatttt ctttatatac agaaaaagta
      721 caattttttt cattagttac acttacgtaa tcaaactcaa atttattggt attcgtcttt
      781 tcaacaacca cacctaaatt atctgattct ataagtattt cagaaacttc atttccttca
      841 actgaagtta agtgttttaa aatatttgga gtcatatcca tattattata tttaatcata
      901 tctaaacact gtttatatat attggcatca atacctccaa cttttgttat acttttagct
      961 ccttttaaaa tacctttatt acttccatca gcaactaaaa cgattggttt ttctttggct
     1021 aaaggagata atgttagtgc atctactaat tgatatccat ttgttatata aaaatctgtc
     1081 gctccattat aaaacttttc cattaccttt ttattggtct caaatctatc atttcctccg
     1141 attctagttg ctttagtttc atttactaaa tcctcattta tcgcagattt gccacctata
     1201 gcgtaaacat ttaaactttc agtcttaaat ggaactgatt tcccatctgt aagtattata
     1261 gctcctttat tttttgcaac tacaggtgct atactcatag catcagcctc tcctacaaat
     1321 ccatttgtaa gcattacctc ttttacacta cttacttcat ttattttttt agctacatta
     1381 taactagttt caattctatt gttaccattt attctttcca cttgaatacc ttttcttttt
     1441 atatcttttt ctactgaatt ttcaatagta ttataaccac caattatata tacttttttt
     1501 acaacattta aactcttatt atctagtcta tttaaagtag cttttggtat ttcacttttt
     1561 ttagtcaaaa gtattggtgc gttaatagct ccagctaatc cacttgatga aagcccatca
     1621 gataaatttt tatctccatt aactaatatt acagtatcat aagtttgtct atctgctata
     1681 agtcctgctg tttcatatct atcaattcct attattgaat cttctttaat ctgtgcattt
     1741 gcagttaaat tattaaaaag aaacataatc aaaactaaaa ttaatatact tagtttttta
     1801 ctgaattttt tgaatttttt attcataata tacactattc ctcctgtaaa ttaaggtctt
     1861 taatttgctt ataaattatc cccttattta caaataataa cataatatta tagtaacgta
     1921 tatattacct taaaattatc agtaacttta tatcaatcat cttagcttat ttatataaat
     1981 ttttttatat tacttttcaa ataacagtca tactaaaaca atttcatcat ataatttgaa
     2041 aattattact atttacacag cttttatcat catcttacca ccatcaaata tatattcaac
     2101 catattattc tcatataaat aagttatata agaacgtatt gtacttccaa ccaacacata
     2161 ttgattagca tcaagcttga cattgtacaa atcacacatt ttttcaataa cttcatcaat
     2221 cataactggc tcataacaaa cctttttaat attatctatt atttcttcca tcttcttctt
     2281 attaatatta attaaacttt ttatatctgt agttttgaca gagtgacttg gaacaaagaa
     2341 atcagcctct aacattctca atttttctag tgtcttaaac tgtgaatcta tatccagtag
     2401 gaagaaaaaa tgatatttag taattatatt ctctggtgta agactatctg caacaaacaa
     2461 aacattatct ggagttctaa caccaatcat ttcaaaataa tgacctggaa gagaaatcgc
     2521 ttcaagttct gtatctacaa ttttgccatt tgaagggata atataatcaa cttcactttc
     2581 ctttgctaat aaaaatttat tcttcagtgc tttacttgga tagccaccat ataaaaatgc
     2641 tgattctaat attggatttt ctgtaaaata tccttcaatt ttagtcgtag caatcttgca
     2701 ttttgtttga ttcttcaaat atgcatttcc tcctatatgg tctgcatttg aatgagtgtt
     2761 tattattaat ttaggattta aattatgttc ttctaataat cttaaaactt gtctagctgt
     2821 atccttatta tttccactgt caattaaaat tgcatcctta ccatctacgt atacacctac
     2881 tattgtaggg agtggaatat aatatgtatt tccttttata tgttctagag ttaatttagc
     2941 cattttataa cctcccttta aatttacttt taattatagc attaattttc tgaaaattcc
     3001 aatcaaattg tttttataat atatttttat ttcttttatt aaaaattgga aaaactatat
     3061 aatatatatt ataatctata tataaggagt gatgttattg aaaaaagata gaataacttt
     3121 ttcagaaatt ttaatattaa ttgtaggctg tatattaatg gccatttctc ttaacttatt
     3181 ttttaatcca catgcaatcg ctgctggtgg tataacagga cttggagttg ttttaaattc
     3241 attattcggt gtagaacttt ggattgtcaa tttactttta aatgttccat tattcatatt
     3301 tgcttacaaa atactttcaa aaaaagattg ttttaaaacg gttttaggaa taatttttct
     3361 taccatagca cttaagctta ctgcaaatat ggctacatta gatataacaa atgatatgta
     3421 tcttgcaatt atatctggtt ctattttaat gggagttggt caaggactta tttttagaat
     3481 aaatggctct acaggtggaa ctgatttaat ggcactccta ctaaataaat attttcctac
     3541 atttagtata cctgttttaa tgggtattgt agactgcgtt gtagttgttt tatcaggtat
     3601 agtaaataga caggttgaaa ttgcacttta ttcaactgtg gctttataca ttttagtaaa
     3661 agttagtgac ttgcttattg aaggatttaa ttattccaaa tcattcacta ttatatctga
     3721 cctttcaaaa gatataagta aaaaaataat ggaagattta gatagaggtg caactattct
     3781 aaaaggagaa ggtgcatata ctggagaaaa taaaaatgta cttttagtag tagttgaaaa
     3841 gaaagaagtt gtcgaattga aaaaattagt aaaaaatgtc gaccctaatg cttttattat
     3901 aattacagat atacatgaag ctttaggtaa tggatttaaa aaaatagaat aactaacaaa
     3961 taaatagcaa gtataattaa catatacaaa ttcaactttt tatatgttat attatacttg
     4021 ctatttatat attatacttg ctatttatat attataattg ttgtttttaa gataaaaatt
     4081 aaatgtatca attattgttt tatacaagta tttccaaatc cgttctcatt tagatagtat
     4141 gcttagaaat tttagaatta ttgagtctaa ctttattact tactttactt tataacaatc
     4201 aaatacaaat ttaaatatta taaaactaca aaataattta aagcatataa tgtcaatatg
     4261 tttaaattat aaaatattca tgtagttata taataaataa tttataaata tagccaactt
     4321 agcctaaata cgtatataat atagctaata tggttcttat acttgatacc atcaataaaa
     4381 ctaaaaatat aaaaaggaga acttaaaatg agtgttagtt tacaaaaagg acaaagagtt
     4441 agcttagtaa aagataataa tcctgtaaaa aatctagtag taggcttagg atgggatatg
     4501 aataaattgg gtaaaaaaaa ttatgaccta gatgcatttg caatagcatt gactaaccaa
     4561 gataaaatga atgttagagg tgacttggtt tattttggaa atcttaaaca cccttctaaa
     4621 gctattatac acactggaga taacttaact ggaaaaggtg aaggtgatga tgaacaaata
     4681 attgttaatt tagaagacat acctgaatat gtacataaaa tagtttttgg agtttctata
     4741 tacaaagcta aaaaaagaga ccaggatttt ggtcaaataa acaactcttt tataagatta
     4801 attgattcaa attccaagca agaattattt aaatataatc ttcaagaaga cttttctggt
     4861 aaagtaacag ttttggcagg agaaatttat cgtagaaatg aagaatggaa atttaatgct
     4921 ttaggaattg gtcaaaatga agagcttcgt gaattaatta atacatataa gtaataatta
     4981 caataaaaaa tttataatga ggagttggtt taaatgggta taactttagc aaaaggacaa
     5041 aaagtaagtt taacaaaaag taaccctggt ctaaaaaaag taatagtagg actaggatgg
     5101 gatattaata aatatgatgg tggctttgat ttcgatttag atgcctctgc atttttaact
     5161 ggaactgatg gaaaagtaac gaatgatgga gactttatat tctataataa tttaaaacac
     5221 gcttctggag ctgttgaata tatgggtgat aatagaacag gtgttggtga tggtgatgat
     5281 gaacaaataa atgtagactt atctaaaata cctcaaaata ttgctaaaat atcatttagt
     5341 gttactataa atgaagcaat tacaagaaga caaaatttcg gacaagttga aaattcatat
     5401 ataagaatat ataatgaaga aactaatgag gaacttatta aatatgaatt gggtgaagat
     5461 tttagcatag aaacagcaat agttgtggca gaactatata gacataatgg agagtggaaa
     5521 tttaatgctt taggttctgg atttgaggat ggacttgcag gattgtgtaa aaattttggc
     5581 gttaatatag gctaatatat ctaaataaaa ataagagtat attgatatac tcttattttt
     5641 atttagatat attataaact gtttacactt ctactcttct ttctcgcttg ttttttccca
     5701 tattctactg aaatatcaaa aaatgctgaa atattttctg taaaaaactt gcttctatga
     5761 tatgcaatat caaatgtcct atgtaaatta ataccttcaa tatcacaagc ccacaaaaga
     5821 tgctttttta aataatcctc aataagtaat tcggatataa ctgcaatgcc aaaattatct
     5881 acaactgctc ttaatatagc ttcagagtta taacatgacc atctaatatt cattgggatt
     5941 tttaattctt ttaattgctt ttctaattgt gctcttgtac cacttcccaa ttcacgcata
     6001 attaatggtt gatttgaaag ttctgaaact tttatagagt ctcttttata aaactcatgc
     6061 ttgtgtgaac atataagtac aagattatca ttaataatgc tttttgtaac aatatctggg
     6121 tgcttaattt ttccttctac aatcgcaata tctaattcac tattaagtaa tttttttgca
     6181 atcagtcttg tatcttctac aaatacttca ggttctataa gaggatttac cttgtataat
     6241 tctaacatta ttggacttat tacacatgta ccaacagtta ctgttgcccc aactcttata
     6301 cggatatttt ttgcacaatg cttcatacaa tcattcattt cttcatttac agctaaaaga
     6361 tgcctcccat atccaagaag ttttttccct gcctcagtta aatataattt ttttgaaatt
     6421 ctatcaaata gcttaacacc atactctttt tctatttctg aaatagcttg gctaactgat
     6481 gactgggtaa tatacatatt tcttgctact tcactcattt taccacattc agcaacctta
     6541 acaaagattt caagactacg aattgtcata ttatcacaac cttagcaaac attagtaaat
     6601 gcttatgatt ttattaatat tttagtattt tacaaatata ttgtcaagaa gtataatttt
     6661 attatagagt tataaagtag attggagtgt gatatattgt gaaagtatta attataggtg
     6721 gagttgctgc tggaacaaaa actgctgcaa aaataaaacg tgaaatgggt gataattgct
     6781 ctgttacaat actaaataaa ggtgatgata tttcttatgc aggttgtggt cttccatatt
     6841 acgttggtaa gataattgaa gataaatcat ctttaatcgt aaatacacct gaaagttttt
     6901 ctaaactaac aggagcagaa gttcgttgtg gagttgaagt tacatctatt gatagaaatg
     6961 caaaacaagt aactttgaaa acgctttcaa caggagaaaa agaaaatcta agctatgaca
     7021 aacttgtaat tgctacaggg gcagacccaa taaaaccacc aatacctgga atagatttac
     7081 ctggagtatt ctttatgcgt actccaaatg atgctataaa actaagagat tctattgaaa
     7141 acggtattaa gagagctgtt gttattggtg gaggtttcat tggtcttgaa attgctgaaa
     7201 atctttctgc aatgggtata cgtgtaagtg ttgttgatat ggctgaacat gttatgcctg
     7261 gatttgatac tgattttgct gaatacgttg aaaatcatat ggctgataaa ggcataatga
     7321 tttttacagg tgaccaggtt gtaggtattg aaggtgaaaa taaagttgaa aaacttcgta
     7381 ctaaaaatag agctattaaa acagatttag tggttatgtc agttggaatc cgtcctaaca
     7441 caggttttct aaaagacaca ggtttagaat ttgcccctaa taatacaatt attgcagatg
     7501 aatatatgct tacaaatgac aaagatatat atgtagttgg agactgtgct tttgtaaaaa
     7561 attctttaac aaataaacct acatggtcac ctatggggtc atctgcaaat catgagggta
     7621 gaatttgcgc acaaaatata tctggaaaat ctaaaactta caatggtgtt cttggaacaa
     7681 cagtagtaaa attacctgag ctcaatgcag gaaaaacagg tctaagtaaa gaaatagctg
     7741 aaaaagaagg ttataatgtt tgctctgtaa ccatagctac tgatgataaa gctcattatt
     7801 atccaggtgc atctaatttt attataagaa tggttgctga aaaaaatact agaaaattat
     7861 taggtgttca agttatggga cctggagcgg ttgataaaat agtagatatc gctgctactg
     7921 caataacttt aggcgctgac ctttattcat tagaaagtat ggaccttgca tatgcaccac
     7981 ctttttcaac agctattcac ccttttgtag tggcagtaaa tgttttacaa aataaactta
     8041 atggagaact tgatagtgtt ttacttgacg aaattgagga ttttgacagt tggactaaac
     8101 ttgatgtttc aaaagctcct tctattccta gtttacgtta tattcctgtt ggtgaaatta
     8161 atggagaaat tgaaggtctt gctaaggatg aaaaaattct tcttatatgt gctaaaggta
     8221 aaaatagcta tatggcgcaa aatcgcttaa gacgctttgg ctatacaaat acgaaagttc
     8281 ttgaaggtgg aatactattt tatccaaatc ttaaggtaat ggaggagtaa atatgagtaa
     8341 tgttacagca gaacaaaaaa aggaagtaaa aggtagaggt tttctaccaa gcagggatgg
     8401 agaacatttt gcagcacgta taataactgt aaatggtgta ataaatgcat cacaagcaaa
     8461 aaaaattgca gaagctgctg aaaaattcgg aaacggtcag ttagcattta ctactagact
     8521 aacagtagaa ttacctggtg taaaatttga agatattgaa gctctaagtg aatttattgc
     8581 tagcgaaaat ctaataacag gtggaactgg atcaagggtt cgtcctgttg tggcttgtaa
     8641 aggtactgtt tgtgttcatg gtctagctaa tacacaggca cttgctgctg aaatacatga
     8701 agaattttac aaaaattggt atgatgtaaa actacctcat aagttcaaaa ttggtgttgg
     8761 tggatgccct aataactgta ttaaacctga cctaaacgat cttggaattg ttggtcaacg
     8821 tgtacctgac tatgattcag agctttgtgt tggttgtaaa aaatgtgctg ttgtagaggt
     8881 ctgtcctgtt aaagcagcta aattaacaga taaaggtaaa cttgaaatag atagtaatct
     8941 atgtaataac tgtggtaagt gtatagaaag ttgtaatttt gatagtattg aagaaaaaga
     9001 atctggttat aaagtctata taggagggaa atggggaaaa tcagttcgtc ctggaacaca
     9061 gattgataga ttattttcaa aagaggaact tatgacttta attgaaaaag caattctttt
     9121 atttagagaa cagggtaaca caggtgaacg ttttggaata actatcgata gaatcggtgc
     9181 tgataaattt atagaaatgc ttttatcaga tgaagttttg gaaagaaaag aagagatact
     9241 agaggctctt ttacacctta caggtggagc tgtatgctaa caataaatat tttaattgta
     9301 cagataacct atggaaaaaa ttttagttaa gaataattaa aattctatat atgatatatg
     9361 ataaatgagg ctatctcaaa acacagatac gcctcttttt cattgttgaa atgataaagt
     9421 atatttgagt tcatataaaa aagagtgtct catgaattat aaaattcatt ttgaggcacc
     9481 cacattattt tataataatt gcttattaat tttattttaa ctatataaaa tgtacaaaaa
     9541 ttaagaatat ccaatattct aaaattaatt ttgaattgta ttgattgaaa agtttactat
     9601 tccactattt ccatcttgat ttactactag ataccatgtt ccagtattag gaactctcat
     9661 tctacatgga tttttctttg caaatccacc atagtaatca taatttcttt gatttttaaa
     9721 tttcacataa tttatatggt ctaacaatat tacattggct tgtttctcta aatttacctc
     9781 tacaatatcc ccttttttta agttcatctt actataacta tattccattg aattactcct
     9841 ccagtatcga ataatctttg ttatttactc atctcaaata tgtaaaaatt catttaaaat
     9901 attacaaata ctatataatt agactattat ccagttttaa ccttatttac tacaatccaa
     9961 aggttattaa aaatattatt ggcaacaact tctccatccc atacaactct ttcttcttta
    10021 ttttctaata aagcatagta cccagagtca gaactttcat ctttatagac tgtaacataa
    10081 aactcttcat cactatattc aggtatattt ttaaagtctt cagatatttt acatgttaat
    10141 atataacctt ctaattcttc atctataaac aaagcaattc ctccaatctc tatattttta
    10201 ctaattatac catattatgt cataaacttt ttattttatt acaaatttct attttttctt
    10261 tactttataa ataaattata taaattttaa cttttaa
//
LOCUS       DAYAOY010000129         9947 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYAOY010000129 DAYAOY010000000
VERSION     DAYAOY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.129"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            251..2485
                     /locus_tag="ACGI6B_003538"
     CDS             251..2485
                     /locus_tag="ACGI6B_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9752947.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            2741..4771
                     /locus_tag="ACGI6B_003539"
     CDS             2741..4771
                     /locus_tag="ACGI6B_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9752948.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            4768..5220
                     /locus_tag="ACGI6B_003540"
     CDS             4768..5220
                     /locus_tag="ACGI6B_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9752949.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            5222..6277
                     /locus_tag="ACGI6B_003541"
     CDS             5222..6277
                     /locus_tag="ACGI6B_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFG9752950.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            6344..6652
                     /locus_tag="ACGI6B_003542"
     CDS             6344..6652
                     /locus_tag="ACGI6B_003542"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFG9752951.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            6756..8021
                     /locus_tag="ACGI6B_003543"
     CDS             6756..8021
                     /locus_tag="ACGI6B_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HFG9752952.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            8109..8825
                     /locus_tag="ACGI6B_003544"
     CDS             8109..8825
                     /locus_tag="ACGI6B_003544"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9752953.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(9020..9313)
                     /locus_tag="ACGI6B_003545"
     CDS             complement(9020..9313)
                     /locus_tag="ACGI6B_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HFG9752954.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(9319..9534)
                     /locus_tag="ACGI6B_003546"
     CDS             complement(9319..9534)
                     /locus_tag="ACGI6B_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752955.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         3858 a         1002 c         1703 g         3384 t
ORIGIN      
        1 tttggccaaa atttaaaaaa taaataaaaa atgatacagt gataaattgt aatacaaata
       61 tagtttcaaa ttgcatataa tataattatt taataaaacc attatctatt gaatgttaat
      121 gaaatgttaa gatggcattc ttgaatatta agaaatagaa tactataata tagtgtgaag
      181 gatttttagt aaaaaaattt gtaagtgtta atatacatta aaaaataggt ttataatttg
      241 gaggaaaaaa atgaaattat caaggtataa gaaaaatagt ttagttctgt ctataatttt
      301 atgttcactt attatttcaa tccttgtaat tactgttttt aataccgtaa aactacacaa
      361 tatatctaat aaacaatcta aaaactattt aaatgatgta tctacacaga ttgtaatgaa
      421 tgttgattct aaaatcaaat ttattttatc cgacttaaga ataatggcag attttattaa
      481 gcaatatgaa ggtgatagtc gatatgagta tttatctaaa agaaagacta gttataagta
      541 ttatgatgtt ggaataatag atttagaagg taaagctgag tttttaagtg gaaaaaagtt
      601 tgacttaaag aatactgttt cctataaaaa aggaattgaa ggtaaagaat actcagaagt
      661 aatagagtca atgggttttg ttctttatag tgtacctata tttaatgata atcatgaagt
      721 atctagtatt ttaataggtg tatccaataa agaaagtatg aatgagattc ttaatataga
      781 taactttaat ggaaaaggaa ctattgaaat tattaatagt aagggaaaac ctcttttcat
      841 aggaaaaaat actgaattaa taaaggacct tagccataaa tataagagtt ctggagatga
      901 accttgggca caaaagatgc ttgaggattt taaaaatgat aaatcaggga atattacaat
      961 aacctcatca aaaggtgtac aatgcttatt aacttatcat ccaattacaa gaggtttaaa
     1021 tgattggcat tttcttctca tagtacctga agatgctgta cttggtgaac ttaataaatt
     1081 aaactctttt acaataacaa tgacatttat aattacatgt attattggaa ttataacatt
     1141 aattttatat attataagaa gaaaatatgt aaatcaaata gaaaatattg catatttaga
     1201 tagtataaca aatggtataa attcaactaa gtttagtatg ctagtcaaac ctttaatttc
     1261 agaatcacct gatagtactt atatgatgat agcaatgaat ataaaagatt ttaagctaat
     1321 aaatgattgt ttcggtagtg aaaagggaaa tatgacacta aaacatcttt acaatatttt
     1381 gtataaaaac attgatgaaa atgaagaatt tgtgtgccgt catgatgcag accttttcta
     1441 tttgcttatt aagaatagac cagtgttaga agccctggag ttattatata aaatagaagc
     1501 tgacacaaat tattttaata aaaataggga aaatccatac tttttaagac tatcagtagg
     1561 tatttacaca attgaaaatc ataatgaaga tttaattaca atacaagacc atgcaaatac
     1621 agcaagaaaa agctttaata aaactcatca aagtgatttt tctttttata gtgatataga
     1681 aagaaaacga ttaatatctg aaaaggaaat ttcaaattta atggaaaaag cccttgaaaa
     1741 taaagaattt tttatgtgct tacaacctaa aattgatata aaaagtggaa aaattagtgg
     1801 agcagagtct cttgtgcgtt ggaaaaattc agaaaagggc ataatttatc caagtgattt
     1861 tattcctctt ttcgaaaaaa gtggatttat atgtaagtta gatttatata tactagaaga
     1921 aacatgcaaa ttgatttcta agtggataaa agaaggtaaa gaagtgttga gaatttctgt
     1981 taatgtatca cgtcaacatc tgaatgataa attgttttta gaaaaatata agcatatatg
     2041 caataaatac aatgtaccaa catgtctgat agatttagaa cttaccgaat ctattttctt
     2101 agaaaatcca gaagctatag atataataga ggatattcat tctaatggat ttaaatgttc
     2161 tatagacgat tttggatttg ggtactcatc attaggaata ttaaaggatt ttaaagttga
     2221 cataattaag ttagaccgtt cattctttgt tagcaaaaat aatatagacc gtggtaaggt
     2281 agttattaag tctattattg aacttagcaa aaggctaggt atgaaagtaa ctgctgaagg
     2341 aattgaagaa ttagaacaag ttgagtttct aaaaaatgtg ggatgtgatt atattcaagg
     2401 ttatgttttt tcaaaaccat tattaatttc tgattttgaa aattttgcat atgaaaataa
     2461 taaaattaaa aatatccatt attaaatgta caagataaaa aatttaggca agttatataa
     2521 ttaaaaatat aacttgtctt ttttatttat atatttaata aaaatatatt tgcagatttt
     2581 gtaaaaataa ttttcaaatg ttgtaagttg tggctaaagc tttattgtat tattctgaat
     2641 atagaaagat agttactgtc ttagataaaa actgaaacat acataataat ataaaatttg
     2701 ctatattaat aactctaatt attttggaag gagggaaaat atgataagtg ggagaatgtt
     2761 actcatcatg aattttctaa aaggaaaaaa taagacaagt tacaaggata ttagcaaaca
     2821 acttagtata gaagaaagaa aagttagata tgatataaat aatttaaata ttgttttgcg
     2881 ttcattaaac aagtcagtaa tacaaaagat gaacaaaggt gtgttaatta ttcctgatga
     2941 cttccaagta gtatgtataa ataataacga atatgtattt tccttaactg agcgagtatc
     3001 tattatgaaa attatatcaa tgtttaaaat agataatcta aacttagaaa aattaagtaa
     3061 agaatttcag gtttctagaa gttcaattaa aaatgacctt aatgttttgt caagtgaatt
     3121 agaaagaaac caaataacta ttacttatga taaatctttt aaaataagtg gagaagaaga
     3181 cattatatat aagcaaagat tgaacatatt aaaatcgtat tcttatcttt ttggtaagga
     3241 aaaatctgat ttaagtgtat ttgagaagta tttagtgaat gtaattgaaa aaatattcaa
     3301 agggatttat ttgattgata tatataattg gtctatgaat ttattattgc aaatgggatg
     3361 gactttaaat gatgaatctt ttacatggta tgtgtcaaac attttccttt ttacatggta
     3421 tatacattca gatgaaaaac atcctttaga atctgcttca ttaagtgagc cgtgttttga
     3481 aaataagttt gaccaagaat ttgaaacaat tattcaaaaa aagttgacta aggaacaaat
     3541 ttttctgtta aagagttttg tattttttac aaataaatat gcaagtttaa acgaggatat
     3601 ggatttgatt agtacagaaa ttatagttca agatctaatt tacaatatgt cagaaatatt
     3661 agagattagc tttaaagaag atatgattct ttataaagga ttactcaatc atattgcccc
     3721 attgatagag agaatcaaac agaatataca gatttatgag gaattacttc atgtaatacc
     3781 agataaatat caatatgtac ttgaagctac taaggtttct attcaaaaaa acagagtttt
     3841 aagtcaaata aagaatgaaa atgaaacaat acttttggca atacatttct taggaagtat
     3901 acatagaaat gagaggaata actacaaaaa tatattgtta gtttgtggat ttggatatgg
     3961 tgtaattgct atgttaaagg atagtttagt aaatgattac cagataaata tagtagagag
     4021 tattccatca tataagttac aagattataa agaatgggaa agtattgact tcattataac
     4081 aacatcaaag atagtaatag acttgcctaa aacaattatt gagataaatc catttttaca
     4141 agaagaggat tatttgaagt ttgaagaaaa agggataaaa agaaagaacg tcttaactaa
     4201 ttatatgtca ataaaaaaaa ggttggactt tttagaagaa gatgcccaaa aaagagtatt
     4261 aagtattata tgtcaagaac ttggatacag tgatgagaga atattattta gacaacttaa
     4321 tttaagtgat ttaataggaa ttgataccat aaggataatt gatggagata tgaaatggga
     4381 agatgctgta aaaattagtt caaacatact tgctaattgt gattttgtat cagcacaata
     4441 tgctgagaat attatagata ttttaaaaaa tgtgggtttt tatgctgtaa aagataacga
     4501 atttgcttta ctacatggca atgatagttc attagtcaaa gtaagttcga taagtttatt
     4561 aatatgtaaa gaagcagtta gttttggaga taaaaaggtt aaaatcatat tttgcctagc
     4621 aagcagagat aaaaaagagc aaattcctgc aataattaat ctaactaaaa tggtttataa
     4681 gactaatttt attgctatgt tagaaagtgc aaagacaaaa gaagaagcag aagttttaat
     4741 tcataaatat gagaaggagg tcactgtatg aataaattag ttaatgtaaa atgttcattt
     4801 aaaatacatg ttaattcaaa agaagaagcc attatatcgt tagttgatgt agtgtcaaaa
     4861 gaaggatatt taatagataa aaatcaattt ttaaaagatg ttttaaagag agaagaaaca
     4921 ttgtcgactt atattggaca tggtataggt cttcctcata gtcaaagtgt aggtgttaaa
     4981 aactcttgta ttacaatagg aaagcttgat actcctattg aatggacaga agaaggagaa
     5041 aaagtggatt taatattttt gatatcagta acaaaagaca atgaaaataa tcttcattta
     5101 aagattttat caaaattagc acgtttattg atgcatgaaa gttttagaaa tcaaattaga
     5161 tgttcagatg aacaaactgt ttataatctt ataaaagaaa aaatagagga ggaagattaa
     5221 aatgagaatt ttaaagtttt tgcaaaaaca tcttatgaca gcaacatctt atatgattcc
     5281 atttgtggtt gcaggaggaa tattatttgc tttatcagta actttaagtg gacaagctgc
     5341 tgttcctgaa acaggctggt tggctaaact gaatcaaatt ggagctgctg gcttggcttt
     5401 attcattcct atattaggtg gatatatagc atttagtatg gcagataaac caggattagc
     5461 tccaggtatg ataggtgcat atttagcaaa agaagtaaat gcaggtttta taggtggtat
     5521 catagcagga tttatagcag gatttgttgt tttacaatta aagaaaatta aattagctcc
     5581 tacaatgaga actttaggtt ctatatttat ttatccttta gttggtacat tgattacagg
     5641 tggtataatt gtatttttaa ttggagaacc tatagcttct tttatgacat ggatgacaaa
     5701 ctggttaaat ggaatgtctg gtgtttctaa aattccttta ggtggaatct taggaggaat
     5761 gatagcctca gatatgggtg gccctataaa taaggttgct gctacttttg cccaaactca
     5821 agttgacaca cttccatatt taatgggtgg agttggcgtt gcaatttgta tacctccaat
     5881 tggtttagga ttagctacat tattgtttcc aaaaaaattt agcaaggaag aaagagattc
     5941 aggtaaagct tcactattaa tgggatgtgt tggaattaca gaaggagcta ttccatttgc
     6001 aacatcagat ccagttagag taataccttg tattatggct gggtcgattg taggaaatat
     6061 tatggctttt ttattaggat gtttaaatca tgcaccctgg ggaggtctga tagtgttacc
     6121 tgtagttgac aataggttgg ggtatattgc atcagtatta actggagcag tagttgttgc
     6181 ggtattaatg aaattagtaa aaaaagacgt gaaagaagat gaagaaattg aagaagattt
     6241 agatgaatca attgaattaa tatttgaaga attataaata taaatggaag gaatttgtat
     6301 aaaaaagtaa atataaaact ataaatttaa aggagataag aatatgaaaa ttgtagcagt
     6361 aacatcttgt cctagtggag tagcacatac ttacatgtcg gctgaatcac ttgaattatc
     6421 agccaaaaaa tttggtatag aaataaaggt tgagactcaa ggaagctctg gaatagataa
     6481 tgaattgtca cttaaagata ttgatgaagc aacttgtgta atattgacta atgatgttga
     6541 gattagaaat atggaacgtt ttaaaggaaa gaaggtagta agaatgagtg tatctgagat
     6601 aattaagaaa tctgatgcat taattaagaa aataaaagat acttttcaat aaaaattgat
     6661 ttattttaca tataactcaa agatgttttt aaagaaattt ttattaaaag catctttgaa
     6721 aatatcttaa aaatagaata aaaggagata tagtcatgaa aaagttacca ataaagactg
     6781 tcgtgcaaag attactaaac ttacaagaag aggggaaaag tgcaactctt ttaggaattg
     6841 gtcctatgtc accaaacttg ttacaagcaa gttttgaact agcaaaagat gatgattttc
     6901 ctttaatgtt tatcgcaagt agaaatcaag tagatgcaga tgaactaggt ggcggttatg
     6961 taaatggttg gaatcaggaa acttttacaa aagatattaa gaaggttgcc gacaaagttg
     7021 gatttgatgg tttatattat ttatgtagag accatggtgg gccatggcaa agagataaag
     7081 agagaaatga ccatttgcct gttgatgaag caatggagtt aggaaaaaaa tcttatttag
     7141 cggacattga agcaggtttt gacctattga tgattgaccc tacaaaagac ccatttgaaa
     7201 ttggaaaagt aattccacta gatgtagtac tagaaagaac tgtagaatta attgagtatt
     7261 gtgaaaacga gagaaaaaga cttaatctac cagacatagg ttatgaggtt ggtacagagg
     7321 aaaccaatgg aggtctgact tcaactgaga cgtatgaaac atttataact agactaaagg
     7381 ttgaactcga taatagaggt ttgccaatgc caacatttat agttggtcaa actggtactt
     7441 taactagaaa gacagaacaa gtaggaactt ttaattttag aaatgcttat gatttagctc
     7501 aaatggctaa gaaatatgga gttggtttaa aagagcataa tggcgattac ttagatgatg
     7561 taactttatt agaacatata ccatctcaaa ttattgctac aaatgtagct cctcaatatg
     7621 gtactgaaga aactagggca tatttaaaat tagcagaagt tgagtgtaaa ttagaaaaag
     7681 agggcttagt tgagaaaact tctaatatca gacatgttct tcttgttaat gcaattgaat
     7741 gtggacgttg gagaaaatgg gtagtaggag aacaaaaaaa tctaactact gaagagatat
     7801 ttaaggatga agttttatct aatgaaatac tagatattgc aggacattat acatttaata
     7861 atgatgatgt taaaaaagaa atagaagtgc tttatgataa tttaagtaag aataatattg
     7921 atggtcaaag atttgttgta gaccatatta aacgtccttt gcgtgattat gcagaatgct
     7981 ataacttaaa aggtgttact acgaggattt taaataaata ataggactta tagtggtgag
     8041 tattgttata ctaggtattt atagttataa ttatacttac cactattaaa tttaggagga
     8101 ctaatatttt gattaattta atttttgatg tagatgatac tttatataat caattaacgc
     8161 ctttttacac tgcatataat aaagtgtttt cttcaataaa ggatatttct attgaagatt
     8221 tatatatgag tagtagaaag tatagtgatg aggtatttca tatgactgaa aatggagaaa
     8281 tgccaatcaa agaaatgcat atttatagga taatgaaagc ttttgaggag ttgggaaatt
     8341 ctattacaga aaaagatgca caaagttttc aagatgagta tatatatcaa caatcacaaa
     8401 ttacactaat accagaagtt gagaggattt taaatttttc taaagaaaga aatattaatc
     8461 taggaattat aactaatggc ccatcaaacc atcaaagaat gaagttaaag cagttaaata
     8521 ttgaaaactg ggttgataaa agtaatattt ttatttctag tgaggttgga ttttctaagc
     8581 cagatactaa tatttttaga gtagctgaga atgttatgaa tttagataga gaaaatacat
     8641 attatgtggg tgattcttat agaaatgatg tattaggtgc taaaaaagct ggttggaaga
     8701 gtatatggtt gaaccatagg ggacatgagt tagaagagtt gttttataaa cctgattttg
     8761 taattttgga acataaggat ttgatatctt tatttataaa aatatgtagt tataaaaata
     8821 tgtagtaaaa aaatgttaat aatagaattg tataaaataa ttaataataa ctatataaaa
     8881 ttatatgttt agattgtgag caatctattt tatgttaaaa atagattatt tagaagaagg
     8941 ggaaatctta aaaaaatttg aatatcccct ttttattatt taattaaaat gaaatagtta
     9001 gaaatatatt ttatttagtt tagtcaataa cattttggga ttctttataa ctacatgtta
     9061 actgttgaat aggtttatca tatggtttaa cccatccttt attaatactt aattctgtaa
     9121 gtgctgtgtg accttctacc atttgagtta atgaagctga atatatagct cttagttcag
     9181 gtgtactact tgtcaatgca gaattaagat acatattggc agatgcttta gcagatgaaa
     9241 gcatacttaa cattattact ttatcgtcta tataagtatt gttcttaatt agattttcta
     9301 tcatatttct catgttatct atacctcctc agaaattagt atttcatttt caactataaa
     9361 ttgttgtata gctttaattc ttccttctgt tgctaatata ctagattctg attgtctttt
     9421 tagatcttca tctgatatta acatatttat tgctctttgc ataagtaatc cgtcagactc
     9481 catttttata attgctgcta aagatatttt ttcagtttct gaaagtattc tcatttaatc
     9541 atctcctata ttgtaattta tgctttaata gtatgtcttt cttttcaagg aaataaccat
     9601 tacacttttt tacagtattt tatattctcg ttatatcaaa atttagtcga cttaatatat
     9661 taatataatt ttgtaggata tttatcatta aatatctata aaataaattg tttgaatatt
     9721 cataaagcaa aatttatttg tatgtacttc ttcaagagta aatatgagtt ttttcaaaaa
     9781 attaattttt atgataatta ttaagggcta tatttttctt aaagttgtga ttatcattat
     9841 taatttacaa taaaattgcc gaaaagccct ctcctttagg tgtagggatg aaaggtaagg
     9901 tcttaatata aaaaagttag aataatgctt gtaatctaat accgcta
//
LOCUS       DAYAOY010000130         9900 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYAOY010000130 DAYAOY010000000
VERSION     DAYAOY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.130"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            39..386
                     /locus_tag="ACGI6B_003547"
     CDS             39..386
                     /locus_tag="ACGI6B_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9752956.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(536..1015)
                     /locus_tag="ACGI6B_003548"
     CDS             complement(536..1015)
                     /locus_tag="ACGI6B_003548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9752957.1"
                     /translation="MAGAFAGGEGDFTTAFEPTATAMEKEGSGYIVTSIGKDSGEIPF
                     TAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKAMQPFFEDISLEDLTTV
                     VDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKDIVNTEFANNAIKNIK"
     gene            complement(1063..2568)
                     /locus_tag="ACGI6B_003549"
     CDS             complement(1063..2568)
                     /locus_tag="ACGI6B_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9752958.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2767..3621)
                     /locus_tag="ACGI6B_003550"
     CDS             complement(2767..3621)
                     /locus_tag="ACGI6B_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9752959.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4006..4287
                     /locus_tag="ACGI6B_003551"
     CDS             4006..4287
                     /locus_tag="ACGI6B_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752960.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4437..5285
                     /locus_tag="ACGI6B_003552"
     CDS             4437..5285
                     /locus_tag="ACGI6B_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFG9752961.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5337..5858
                     /locus_tag="ACGI6B_003553"
     CDS             5337..5858
                     /locus_tag="ACGI6B_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9752962.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            6227..6742
                     /locus_tag="ACGI6B_003554"
     CDS             6227..6742
                     /locus_tag="ACGI6B_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9752963.1"
                     /translation="MNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQ
                     DLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQE
                     LSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRALK
                     KLELYLEGGKL"
     gene            6739..7500
                     /locus_tag="ACGI6B_003555"
     CDS             6739..7500
                     /locus_tag="ACGI6B_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752964.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            7538..8404
                     /locus_tag="ACGI6B_003556"
     CDS             7538..8404
                     /locus_tag="ACGI6B_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752965.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            8425..9666
                     /locus_tag="ACGI6B_003557"
     CDS             8425..9666
                     /locus_tag="ACGI6B_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9752966.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
BASE COUNT         3693 a         1242 c         1365 g         3600 t
ORIGIN      
        1 taatagtaaa tctaatatag aaaagaggta tttctagtat gcatgaaaat aaaaaaaatc
       61 tattagacgg aagcttagct caatataatg atacagcagt tccaaaaata ccagtatttg
      121 ctggtaacga tataacttca gaagtttatt attttaaacc taatcaagtt cttaatgctc
      181 atagacaccc taatggtgaa caaatatttg tgttcctaaa aggagaaggt aaaatgaaac
      241 ttggtgagca tgaatgtgat gtaaaaaacg gcgatactgt atttgtacca acaggagagt
      301 ggcatgaaat tactaatgga agcaatgaag aaatggtagc cgttcaaatt acaaaaatta
      361 atgctggagc agaatacaga ggatagatat ataagtacag ttagtacttt aatatctatt
      421 ttattatata aactttaatc tcaaatataa aaagagtctt ttaaaaggct ctttttatat
      481 ttttgttaat tttcattata aaaacaagaa aatatctagc taaaactaat atattttatt
      541 taatattttt tatagcattg tttgcaaact cagtatttac gatatcttta taaggtgctt
      601 ccttatctag ttctttagct tctttcataa cagtcatcaa tttcttcaaa ccttcctctt
      661 ttaatacagg attttgagac catgcatcta ttgatttata tctatctaca actgttgtta
      721 aatcttctaa agatatatct tcaaagaaag gttgcatagc cttagctact tctttgctag
      781 atgcttgttg aatccataat tgacctttat aaacagcatt tgtaaatctt tgaattaaat
      841 ctttattgtt tttcatgtat gatctcgtag tagagtatgc tgtaaaaggt atttctccag
      901 aatcttttcc tatagatgta actatatatc cactaccttc tttttccatt gctgttgctg
      961 taggttcaaa tgctgttgta aagtcaccct ctccacctgc aaaagctcca gccattacac
     1021 caaattggta atcagtctat ttataacatt taaaacatat aattaatctc ttgggatatt
     1081 agggtaaagt attaaagaaa aatcatcaga tttttgatta ggttcttttt tatagactgc
     1141 gtaatttatt acacttttaa gcaatatatt ttttagataa ggttcttttt cctctgtata
     1201 ttcacttaaa attttttcaa gttgaggaac aatttgtgtt ttaactgtat ttctttcctt
     1261 ttgcatttca atatctatat ttgttttatt aatagcttct cttgcatttt ctatacgaga
     1321 taagagtatt tttaaacgtt ccccaaatgt attactgtca taaattcctt gttctaaaaa
     1381 atcatgtagt cgttcttttt gtttttctaa agtatccaat tctttattta aatttatcaa
     1441 ggtatgttct aaagtatttt caggtttaga gttgtctttt tcatctttta aagatagtct
     1501 ataatttact aaccatttat ttaagacctc taatatttta ttttctaata tgtatagttt
     1561 agtagcttta tttttacaat tagtacaaac catatatttc tcatatccat ttgatgtagt
     1621 attagatttg gctattaaag tatgattaca gtatccacat tttacaattc cagccaatgg
     1681 atttgttatt ttattgtttg gagtagggga attaatacgt ttttttctta tattttgtgc
     1741 cttattccat atgtcttcac ttattattgg ttcatgcttt ccttttacct caatttgttc
     1801 ctcaattcct cttgttctac tagaatttct tttcctctcg actttattcc atactatata
     1861 tccacaatac actttgtttt ttataatatc tcttataccc ttttcatacc aagttctacc
     1921 tgttgtggtc ttataaccaa gttcgtttaa ataattggat attttacatg caccatctcc
     1981 ttttaaatac atatcaaaga ttattttaac aacagttgct ttatcttcat ttattacaag
     2041 tactctttct ttattattta aatatttaat atcataccca taaggaggag cttgagctat
     2101 aaatttaccc tcttcaatac ttttgatttt tcctctttgc atacgtctag atattaattt
     2161 taattcctta cgagccataa aagcttcaaa ttcagaatac tcctcatcaa actcatcatt
     2221 taaatcatac gtttttctag gagtaataat tttcgtttta gattttttaa atgtatctag
     2281 tattaaccct tggtcttgca tattaccacg acccaaacgg tcaatatcca tcactaaaac
     2341 agcatcatag ttattatttt taatttcacc tagtaattct agcatcttag gtctatattc
     2401 tatactttcc ccagaaacaa gttcttcttt aatttcaatg acatttaaat tttgttctct
     2461 tgctagtttt aaaagagttg tcttatgtct acttagagtt tcaaattcac ctctatgttc
     2521 tgcttcttca tcagctctgc ttttgcgtaa ataaattgcc acattcatta aatcacaccc
     2581 cttgaatata ttatattcag taacagattg attatattta aaacacaaaa ataaaccttc
     2641 aaaatacaag atatattttt taggtttatt tttatacagt aaattttata gtatatttaa
     2701 aagcttgtaa tctaagtaaa tatttatact attaatttta tgctttcttc attttttaat
     2761 tttaaactat ttttttacta caggtatcca tacttcaaat ttagcatttt gagggtctgg
     2821 atttaagtac agttcaatat caggaccatt atcatattca taaccagaag taggtaacca
     2881 ttctgttaca atacgctttt ctagttcttg tattgcttgt ggacattgtc cttctccaga
     2941 aaatattgcc caagtaaaag ctggaacagt gtattcttct aatgtattat ctatagattt
     3001 tgtacttgaa acagatataa aatacttcca ttcttctgaa ttattacaaa tacttatacc
     3061 taatacacct tgaagttgac tatccatcat agggattaat ttttgaagcg ttccatctaa
     3121 agcaaccttt tgccacattt ggggaacaat ttcaaaattc ttttctagtt cattatgaag
     3181 tggctgagat acaccaacta tacgaaatgc ttctttcttt tcaattctat aattcatttc
     3241 ttcggctcct ttaatactaa ttttaaaact gattggagga aaggctttta aagttgttat
     3301 ttctcttttt tttataactg atggggcaac accatgaata cttttaaatg cacgattgaa
     3361 tgctgttgga gaagagtatc catattttaa agccacatca attatctttt tatcatcatt
     3421 ttgtaattct acagctgcta aagacatccg tctacgacga atatattcag aaagtggtac
     3481 atctgccata taagcaaaca ttctttgaaa atgatatgta gaacaacaag ctatttttgc
     3541 cacttgttca tactcaattt cttttgttat gttttcttca atatagttaa ttgctttgtt
     3601 taatctttca atccattcca taaaatcagc tccttgtaat ttatataata tctattttaa
     3661 aatagttaat cctctctttc tatgcactaa ttaactagct taataaagaa tgaacatgag
     3721 aaagtctgta attaactaca aaaatgaaca aatagtattg tttttcacat taaagtatat
     3781 cacaaattag tatctaattt ttatataaga taacagtttt aatttattat gaaattttta
     3841 taatatttta gcttagacat ttaattatat ataacatttt atttatagtt aactattttt
     3901 gaagtttacc cttaatcaag caacacttag ttattcaatt ataaataatg attgagaata
     3961 ggacaatttt taagatacat aaaatataaa gaggagggga tttaaatgcc aaacatagaa
     4021 aaagacttag atggtacaac tactataaca tacctcaacg acgatatagg tgttccaaca
     4081 gacgagaatg gatataaaat atctatgaca atactacatg gaaagacaat ctgccatgta
     4141 gtatctcctg aaacaatctt aggaagaaag ttcacattag aagaaaaaca agaaaaagat
     4201 acaataatat atggagcaat agaaaaaata atggtagaca gacaattaca aataaaaaat
     4261 tcaaacaaag aagttgctag tcattagcac ttctttaata agtaaagtag gacaagtatt
     4321 ttttataata ttattgactt atagtctaat tagactataa tataaacata gatagtctaa
     4381 ttagactact attaaaaata aatacaataa gaatttaaga aaggataata ataataatga
     4441 ttaaatcatt tttaatgtta ggacagtcca atatggctgg aagaggattc ataagtgaag
     4501 tgactccaat ttataatgaa agaatacaga tgttacgtaa tggaagatgg caaatgatga
     4561 cagaacctat taattatgac agaccagttt ctggggtaag tcttgctgca tcatttgcag
     4621 atgcatggtg ctgtgaaaat caagaggata gaattgggct aattccttgt gctgagggag
     4681 gcagttcctt agatgaatgg aatatagatg gtatactttt taaacatgct ataagtgaag
     4741 ctaaatttgc catacaaagc agtgaattga caggaatttt atggcatcag ggagaaaatg
     4801 acagtaataa tggcaactat aaattttatt ataaaaaatt actttctatt attgaaactc
     4861 ttagaaaaga attaaatatt ccagatatac caattattat tggtggattg ggagacttct
     4921 taggaaaagt aggttttgga aaaagttgta ctgagtatgt atttatcaat caagagttac
     4981 agaaatttgc ttttgaacaa gataattgtt actttgttac agcaacaggt cttacatcta
     5041 atcctgatgg tattcatatt gatgcaatat ctcaaagaaa atttggatta cgttattttg
     5101 aagcttttca taagaaaaag catattatgg aagcattagc taatgaaagt gagttgataa
     5161 taccgagtaa ttccaagaca tatacaaaaa cagaaaaaat atatataaat agtatggact
     5221 tggcactagg aaagatatca tatgatgagt ttgaatctaa acttattaaa cttaatgata
     5281 attagataaa atattcatta atatactaat aaatatagat tgaaagaggt gattttttga
     5341 taccattatt aattttagga ttattaaaac aaaatccaaa ttcttatgga tatgagttat
     5401 tagcattaat ggaaaagaag cattacaagt acatagtaaa tttcactaaa ggttcttttt
     5461 attataattt acagcaactt gaagaaaaaa agtttattga aaaagttgaa aataaacaat
     5521 ctaataaaaa tagagaaact cataactaca taattactga acttggatgc caggaatttg
     5581 aaaaattaat gtataagtat ggttctaaaa cagagtatat aaatctatca ttttacgcaa
     5641 cactattatt tgcaaatgat tataaaaatg aaaagttaat agaattattg gaaacgcaaa
     5701 ttaagcaaac tcaagtaaga atttctctat taaatgaatc catagaaaat gacaaaacaa
     5761 attcacatta ttttaataga atgctagaaa attctcgttc tcaccattta gttaatattg
     5821 aatggtttga gaagttgcta aatgatatta gagattgatt ttaataataa aaaaacatac
     5881 atatatattt tatataaatg tatgatatat agattaaaaa taaggcagat aattttcaat
     5941 tgaatatctg tctttttgtt atataagatg ttaaaagaaa cttatactat gtaagtaaaa
     6001 tttataaata taaagttgtt cattgttgaa ttacagacac ttaagatgaa aagtatgaca
     6061 tttgatataa taaaattgtt taagtattaa ttatgagtat taattaatgg tatagataat
     6121 tttaatataa ctgtatttat aaataattat aaaataataa attaattttt ttatactgac
     6181 ttttttatag tataaatcgt tatatataat gacggaggtg ataatagtga atgatgctcg
     6241 gattatatca caaataaaag caggaaaaaa agaattgttt gatactctaa taaaaaaata
     6301 ctatgatagc atctactgtt attgttatcg ccatgtagac aataaaatga ctgcacaaga
     6361 tttaactcaa gatgtatttg taacagttat aaataatata gaaaattata aacattatgg
     6421 taagtttgaa aactatttgt atgttattgc aaaaaacaag tgtaaggatt tttggaagaa
     6481 aaaacaacca atatatttag agtcattaga taaggagata tcaaatcaag aattatctga
     6541 tatagaaaat aaaatgttta taaaagaagc tttgactaaa ttaccagaat tagaatttga
     6601 agtgataatt ttacggtatt accatgattt aagatttaag gatattgcta aaattatgga
     6661 gtcaagcgtc tctgtcacaa agtacaggat gagtagggca ttaaaaaaac tagaattata
     6721 tctggaagga ggtaaattat gaataataaa gaaataaaaa gaagtctaca gaagtataag
     6781 gttcccaaat atgattcttc agctcttgaa aagactatta aaactataca tgagttaacg
     6841 cttagtaata cttataaaag aatgaataat aaagactttt ttatatctca aattggtttt
     6901 ataagaaaaa gaacttggat tatgcaattt ctaatactat gtacaatatg tatattttta
     6961 tttagtacat cagcagatag taattcattt tataatagtt tatccttgat ttctatagca
     7021 tcaccacttt ttgtactgac aaatatagaa gaattaacaa aagtgtataa tagtagtatt
     7081 ttagagatag aattttcaac aaaaaataca ttacataaaa ttattgctac tagaatgctt
     7141 atatttggaa taagtgattt attgtttatg attattattt tggtatctgc aaaacaaatc
     7201 attgatgtaa acattctgta tataataatt tatatactag ttccttttaa cctaatgtgt
     7261 ataggatgta tggaaattat aaatagaaat aaaggtaagc atacaaatta ttattgtatg
     7321 atatacggta tatctcttgt attattaata gttacactga gtaactttag agataatcta
     7381 tatcaatttg aagcaataaa tcaatggcta attgtttttc tagtcacagc agtaacatta
     7441 atatatgaaa taagaaaaac gtggagacat ctttgttcat ttgaaaatat tattatctaa
     7501 gtaaaaaata agaatacaca gtatcaggag gaatagtatg gagttaagtg tagaacgttt
     7561 aacaaaacag tataacaata aaattatagt agataaaatt gactttaaat taaatgaggg
     7621 tatatatgga tttttaggag aaaatggagc tggaaaaaca actcttatga gaatgatttg
     7681 cggaatatta agtcctacat ctggagaagt aaaggtggat ggtaaaaata atttggatat
     7741 gggagaagat tttagagata tgattggata ccttcctcaa aactttggat actacccaga
     7801 ttttactgcc aaagaattta tgctatatat agcatcatta aaaggattaa tgcctagcta
     7861 tgcaaagaga aaaacacaag aattattaga aatggtagga ttatcagagg taattaataa
     7921 aaaaataaga acattttctg gaggtatgaa gcaaaggcta ggatttgctc aaacactact
     7981 taatgacccc aaaattttaa tattagatga gccaacagca gggctagacc ctaaagaacg
     8041 agttagattt cgcaacctaa tttcagaatt ttctaaggaa aagattgtta tattgtctac
     8101 tcatattgtg tctgatgtag aatatattgc agataaaatt ttaataatga aaaaaggaag
     8161 atttgtttta actggctcaa tagaagaagt aacttcaaag ataaaagata aggtatggaa
     8221 atgtgtggtc aatgagaatg aaatagatta tatgtctgat agatattgtt tagcaaatat
     8281 ccatcattct ggtggagttg caaatcttag aattgtaagt gatataaagc cacatgacaa
     8341 tgcatgcaat ttaaaaccaa cattagaaga tttatattta tattgtttca acgaagtact
     8401 ataaatatac ataaggagat aggtatgtct acattaatta aatttgagtt aaaaaaaata
     8461 ataaaaagaa agtctacaat agcagttatt ttaggaagtt tattcttaag catcattctt
     8521 tttgcattaa tgccaattca ggaaaaagct aatgatgaaa gtggaaaaga gtataggggt
     8581 ttacaggcta ttgagctaaa aaagcaacaa tctaaattaa atgagggata cataacagat
     8641 aaaaaagtaa aaaactcaat cagagattac caaaaaatgt ttaataatcc aaataatatt
     8701 acaacagatg aaggtgggtt ttcaaatttt aaaccagata tttattggaa attcttctat
     8761 ccaaataaag ctttttataa ttttataata gagtcatata gtaatccaaa tgattataat
     8821 ccagaaataa ttaaatcaat gcctttagaa gatggagtta atttttataa atttagaaat
     8881 gaaaaagtaa atacaatatt aaatatggaa tatcctgata aaaattacac acagaaagag
     8941 aaagattttt ggttgtctaa aaataaggat atagaagagc cttatttaaa tggatattat
     9001 caaggatgga attattttct attggctaga gatttactaa tttttactat agtagctgtc
     9061 tgcataagta cagcttctgt attttcagga gaataccaat ccggtgctga ttcaataata
     9121 ttatcttcta aatatggaaa aacaaaagtt ataacagcta aaatagtaag ttccttttta
     9181 tttggtacga ttatttttac aataaataca attgtgtcta ttggtatact tcttttatct
     9241 tttggaattg atggatggaa tttaccatta caacttacaa ccagttcaat tccataccca
     9301 tatacatttt tacaggcaat attactaagt gtgttaatta tttatatgat attaatacta
     9361 ttaattagtt ttacactttt attatcatct tatatgaaat caccttttcc agttttaatt
     9421 acaaatataa tattactatt tgtaccaata tttttaaaat atagtgaaac aaataagttg
     9481 tataatcaaa ttctcaatgt tttaccatca aatgctattt cattttcatt tactaattat
     9541 gtagattata aatttggtaa tttaataata gctttaccta caatgattat ttttacatgc
     9601 ttagttctta cagttgtttt tttacctttt attaaaaata attttaaaaa acatcaagtt
     9661 aactaaaata tatattataa aaagaaggtg tcttaaaatg aactttttag cttattaggc
     9721 actcttcttt gtattaattt aaatatattt tagtattaga ttaaatatgt tttcatcatt
     9781 ttaacaatga aaaagagctt tatctctatt ttgagatagc tcttataaag ttaaatatta
     9841 aattgataac tataaaatat gtagtttgaa tttataattt gtgttataat catactgatt
//
LOCUS       DAYAOY010000131         9774 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYAOY010000131 DAYAOY010000000
VERSION     DAYAOY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.132"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            151..876
                     /locus_tag="ACGI6B_003558"
     CDS             151..876
                     /locus_tag="ACGI6B_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9752967.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(1004..2200)
                     /locus_tag="ACGI6B_003559"
     CDS             complement(1004..2200)
                     /locus_tag="ACGI6B_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9752968.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(2203..3228)
                     /locus_tag="ACGI6B_003560"
     CDS             complement(2203..3228)
                     /locus_tag="ACGI6B_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9752969.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(3300..4430)
                     /locus_tag="ACGI6B_003561"
     CDS             complement(3300..4430)
                     /locus_tag="ACGI6B_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9752970.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(4658..4839)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4892..5785)
                     /locus_tag="ACGI6B_003562"
     CDS             complement(4892..5785)
                     /locus_tag="ACGI6B_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFG9752971.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(5865..6767)
                     /locus_tag="ACGI6B_003563"
     CDS             complement(5865..6767)
                     /locus_tag="ACGI6B_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFG9752972.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(6817..7977)
                     /locus_tag="ACGI6B_003564"
     CDS             complement(6817..7977)
                     /locus_tag="ACGI6B_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9752973.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(8133..8438)
                     /locus_tag="ACGI6B_003565"
     CDS             complement(8133..8438)
                     /locus_tag="ACGI6B_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9752974.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(8536..9510)
                     /locus_tag="ACGI6B_003566"
     CDS             complement(8536..9510)
                     /locus_tag="ACGI6B_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFG9752975.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         3174 a         1785 c         1073 g         3742 t
ORIGIN      
        1 cttaaaaata tattctgata tattgaaaaa agtactttca agatgatatt ttatatatta
       61 tttagaagtt ttttatttat tgttatataa ttaaatatat gactattttg ctactacata
      121 tataaaaata aatttatgaa ggtgatttta atgaaaacta tttctaaaca agaattagaa
      181 aaaacacttg agaaacatta tttatggctt aaaaacaata atgctggaga aaaggcagat
      241 ttaaaagaat taagattaga aaatatggac ttaagaggat actgcttaag taatattgac
      301 ttttcgtggt ctgaccttat caatttaaat ttagaaaaag cagatttaga aaactctatt
      361 tttaataatt cttatctttc ggattgttca ttaaaaaatt ctatattaaa aaatgcagac
      421 ttaagtggtg caagttttag attttgtgac ttatctaact cagatattag aggagctaat
      481 ttagaaaatt ccaatttaga atatgctact ttaaatggag taatatcaga tgaaagtact
      541 cgttttttta aattgtattg tcctgaaaca ggagcattta taggctataa aaaatgtttt
      601 aattttagaa tggtacaatt attaataccc agcgacgcaa aaagagtgtc tgctacatca
      661 aatgcttgta gatgtgataa ggcaaaagta ctgtcaatta ctagtgtaga tggtaaagaa
      721 tcttttaaaa gtgctcgtgc atatgctgat gaaaatttta tatatagagt aggagaaacg
      781 attgtagctg aagattttaa tgaaaataga tggaaagaat cgactacagg gattcacttt
      841 tttttaacta gagaggaagc tattggttat ctataaaatg ctaaagtttt ataaatatat
      901 aaatacataa acaaaataac aaaagagtat agattgtaat ttcaaaaaaa ttataatcta
      961 tactcttttt tctataacac ataagtacat tttatttcat aaattataat aaattattta
     1021 tatctgcact attgattatt tgagaaactt tatctatatg ttctatctta ctatccaaac
     1081 tgtcataata cttcttactc tcatctctat ttctaaaaga ataaactttt ttatccaatt
     1141 ttaaagcttc ttttataata tctatattcc tcttatttgt ctctcctaca ctaaatcctg
     1201 tatctattat aatcttggaa tcatttaaat ttctaatagc caaatcgaaa ctctcatcac
     1261 ttataggctc aaatggattt tctgtaaaca tctttatccc catagttcta ccaatttcat
     1321 aatcaatatc attttcatgt attattccag aatacagacc tatacctttt ttagtaaaag
     1381 ctctataaat tggtgttgct ttacccccac caccaattat aaaaacttca tttttagatt
     1441 tattactaat ttctactgaa cctaaaagac tattaaagtt tttatcatcc aattcataaa
     1501 gcgaatttat aatatcctca tctactacat cttcaggctg gccataagct atgactttat
     1561 tattttttat aagagcaact ttatcacaac tttttaatgc tatgtctatt tcatgcaatg
     1621 aaagtattac tgatattgac ttttctttac ttaatttttt cagtatgttt atgagttcca
     1681 atctatgttt tatatccaag tgagttgtag gctcatctaa tataattatt tctggttctt
     1741 gaacaagagc ccttgcaacc aacacctttt gtttttctcc atcacttagt tcatcaaaat
     1801 atcttttctt taaatgtaag gcatctacag actccaatgc ttcatctacc attattaaat
     1861 ctttcttaga tagactccca aaaaatcctg tatatggata ccttcctata ttgactatat
     1921 cttgtacagt cattaaatca cctaataatc tatttgtaag tactaaagcc attttttttg
     1981 ataatgcttt tcttgatata caatttatgt ttacaccatt caaatatatt tcacctttta
     2041 ttggtgatat aagtttagat aaggacctta atagggtagt ttttccagct ccattacttc
     2101 ccaaaagaca tagtatttct ccatttttta cttctacatt tatattactt ataacaactt
     2161 tgttattgta tcctacactt aaattattag ttttcaacat gtctaagccc tatccttcct
     2221 tttcaccaat aacattacta caattggagc tcctaataaa gatgttattg tacttattgg
     2281 taattctact ggtgataaaa ttactcttga tattaaatca caaagagctg taactatact
     2341 gcctaaaaat attactactg gtatcaaaac tctattatct gatgttttaa atatcatttt
     2401 tgaaatttgt ggcactgcaa gtccaataaa ccctataggt cctgcaaatg ctgatactat
     2461 tgctgctaat atactagata taaatactat caaaactcta aaaaatctta tatttatgcc
     2521 aatacttttt gcatactctt ctcctagtaa aaaagcattt aaaggtttta ttatgaatac
     2581 agtcatcaaa agacctaata taccaactat tataagtatt tctacctttt cccatgtaaa
     2641 tccagaaaaa cttcccatag accacatagt aaattctttt aatttatcac tatttgcaaa
     2701 tgattgtaac acatttgtta taccactaca tacatatccg gtcataattc ctattattaa
     2761 aagagttgtt atattcttta ccttatttgc aaataaaatg actatagaca ttacaattaa
     2821 agctcctata aaagctgcta caaatatact cattgaattt acactctcaa atcccaatgt
     2881 gtatcctccc aaaatcatga gagctactga taaagtagaa cctgaagata ctcctaatat
     2941 atacggttca actattgggt ttttaaaaaa tatctgtaat aaaatacctg atactgctag
     3001 gcatgctcca cctaccatgg ttgcaagtac tctagggatt cttatattgt atattatttt
     3061 ataatgttct gtattatcca tacctcttaa tacaacatct attacttctt taaattcaat
     3121 ttttacagag ccaaatgcta cacacactaa aaaagtaagt atcatactta taattaatac
     3181 tataaatatt gtcgtattcc ttattctcat tttattgata aaatccattt gtatactcct
     3241 tttagcaaag ctatattaac taaaatctat aaaacctatc tttttgtgtc ctctctgtac
     3301 tatttatcat aatttatata atgtttgatt ttttcacctg tagtacctgg gtggaacatc
     3361 tcaactaaat ctactattaa ttcgtctgtc ttatctactg attgataata atctggagca
     3421 aacacccata ggttcccgtt ttttacaaca tccaaatcag ccaatacagg tgcactagat
     3481 tttatagaat ctaaatttgg tgcataatct gaacttgatg agtatacaaa tatatctgca
     3541 tcttttgcaa cttcatgcaa ttgttcaata cttactcgcc cttcatttga atctattgaa
     3601 gcacatgcat ttattccacc agccattttt atcatattat ccacatatga atctttagga
     3661 gctatataag cacttccttc atatacagat gcccatacaa ctttaggttt agctttgttt
     3721 tttatttttt tacttacttc ttctaccttt tgtacagttt tatttagttc actagtagcc
     3781 atatcttctt tatcataaaa tgcagctatt aaacttgtcc actccattct tccaaatgga
     3841 ttttgctctt tatactcatt gtctacaaca taattaagcc ctaattcttt taatttgtca
     3901 ataaattttg tatcagcctc tgataaacca ctactcacta tagttaagtc aggatttaag
     3961 tctactattt tttcataatc tggtgctaaa tttgaaccaa cgtaatgtat ttttccatct
     4021 ttcattaaat ctttaatttc tttaatagtc cagtactgct cttgtgttgt tacggctttt
     4081 atactatcta gttcatctat tgttcttaga gaacaagcct gtgttgtaga tgctattaat
     4141 acattgtcca caggcgttct aactatatta gcatttttat atttttcagg aattttaatc
     4201 ttctttggga ttaaaatcag ttctcgatta tctccatcag ttactttttt aattccatct
     4261 tctaaatctt caacactaaa tccactagca tattttaatt ctaatttctt accagtacta
     4321 gcttgtttat tctctttgtc acttttacta caccctataa gacctcctac cataaaaatt
     4381 accatagcaa ttaaaaaaaa ctgctttaaa acatttgatt taaatttcat ctctttcact
     4441 ccaatatctt attttttatg agtggctaaa aaactttaat aatatatgct ttttaaagta
     4501 tacttattaa agttatccta aagtcatctt taataagtat aaccttttaa taaaataaaa
     4561 aacttctcct aatttaataa ggagaagttc atatatcaac tttacaaaaa cgaattgata
     4621 cttcatttta ttataaatta caatctccac cctaaattga acttatataa tactgggcag
     4681 gtctcctgac tcacggcttt tcctccatca cccttcccaa aatttttata aatttcagtg
     4741 gattttgatt tcgtatccgt atacagtagc gggggctgta gtgaattttc atcactttcc
     4801 ctattaaact aatatagtac cctagtatta ttttaattta atttttatta agataattgt
     4861 aatttattgt tgacctcttt gcaaggttga tttaacatat atttaattca ttcatcaatt
     4921 ttttaatttc acatacttgt ttttcatttg gttttacaaa tggagcagtc atataatcac
     4981 ttatttgtaa ccctctgata ttcatagctt ttttcattgc tggtataaat ggattggtta
     5041 ttaagtaaaa atccattgct ttattaatat atttttgaat ttctataacc ttctccatat
     5101 ctctattatt gtaagcttca gcccatttgg agcaaacttc tggaattaaa tttgataacc
     5161 cccctataca acctgctccc ccagacatta gattatgtat aaagttttca tcataaccac
     5221 tataaacctt aaattctggg aactctattt tcatagtatt tattaaatct ctggtatgtc
     5281 ccatcaaagt tactgtatcc ttatatccaa ctatgttttt atgttttctt gcaagtctta
     5341 atgtaagttc tggacttaaa tcataaccag ttctatctgg ataattgtac aaatatattt
     5401 cagcctctgt attcttagct atttcactat aaaataactc aatactatca tcatcaagag
     5461 agaaataata agggcttatt atcataacac catctgctcc actattatgt gcatagtttg
     5521 aaagttctat agattcattt atacacatac gacttgtacc tataaacact tttactctct
     5581 tatttacatg actagttact aaatctataa gttctttttg catatccata gacatattaa
     5641 agaactctcc agtacttccc attatgacta gtccatctac acctccatct ataagatggt
     5701 catacacttt tttattacct tctttatcta aatttccttc ttcatcaaat attgtaacaa
     5761 ctggtgtcaa aaattctgct ttcataattt aaactcctaa aatttatttt aatatgttca
     5821 ttaatacatc agatttagct ttaatattaa ttatattaaa acagttactt aatgtaattt
     5881 tttaaaattc caatactctc aatctcacat tcaactacat ctccactact catatatcga
     5941 ggaggtgaaa atcccatccc tactccagaa ggtgttccag tagctataat gtctccagcc
     6001 tctaaagtca taccttttga aagttctgaa actatgtctg aaacgtctga tatgaataac
     6061 cctgtattag aactttgtct ttcttcacca tttaccctac tacatacttt aagttttatg
     6121 ggaaaaggaa ctgcactttt atgtaaaata catggaccta aagaggtaaa tgtatctaat
     6181 ccttttcctc taaaccattg aacatgttct ttttgcaact ttctagcaga aatatcattt
     6241 ataacagtgt aaccaaagat ataatcttct acctcttctt ttctaatatt aacacctgtt
     6301 ttacctatta ctactccaag ttcaacttca tagtctagtt gattatttat ctcaaaatga
     6361 ccatctatct catcttctgg acctattgcc tttgtcacac gttttgtaaa ataaaccgtt
     6421 ttttgtggtt cttcaaaact atcatcaaag tgagtttgag tttcttcaag atggtcttta
     6481 tagttcactc ccacacataa gatatcatgt attggttttt ctataggaac atgtacagta
     6541 catctctctc ttttaaaaac atcataatta tagaatgtac tttcatttat aatagttttc
     6601 aacttaataa tatcttcact agatacattt tgaattaatt catttaaatc cttaaaattt
     6661 ctactgagat ttaaagagtt aacatctata ataaataatt catctttaga aaaaactcca
     6721 gttcttttac attcattttc tccttcagta aaacttacaa acttcataat ctcacccttt
     6781 attaaaattc tatgtattta ttttcttact taaagattat acatagttgc atactctagc
     6841 tattgccatt tcagtatatc caagggactc tgcttttgat tgttttccat ttgctacatc
     6901 tacaaccata tttaacaaat catctgttaa ttcttccatt gtttgtggac catatataac
     6961 tggactagca tcaaaatcca gattatcttc catatttgca aaagttattt tatttccagt
     7021 tatttttatt actggtgcta ttggatttcc tgatggtgtt cctcttcctg tactaaatac
     7081 cactatttgt gctcctcctg ctaccatacc agcaacagat gaagcatcat ttccaggggt
     7141 atccattata acaagacctt tcttatcaat ttgtttggca taatcatata cttcacttat
     7201 ttgtctatga ccacctttat gtatacaacc aagtgatttt tcttctaaac ttgttaaacc
     7261 accagctata tttccaggag atggattacc ttctctaact tcttctccaa ccaattttaa
     7321 tgaattttca tatctatgta ctatatgtaa tattttttct tttacttctt catttactgc
     7381 tctcctagct agtatatgct ctgcacctat aaattcagta gtttcactta aaattgatgt
     7441 tgctcctaaa tcgacaagtt tatcacttaa ttcacctatt aatggatttg ctgctaatcc
     7501 acttgttggg tcagacccac cacattcagt tcctagtata agttcagata ttggaaattc
     7561 ttcttttcta agcaaagaag cttcttgtgc catttctcta gcataacgaa cagctttttc
     7621 tatagttttt aaagttccat gttcttcttg tattacaaga gtttttattg gcttatttgt
     7681 tctctctttt attgcatcaa ctaccaaatc attttggcaa ttttcgcatc ccaaagatac
     7741 aactattaca ccatatacat ttggatttgc tgcataacca gccataacat ccatagttaa
     7801 ttgttggtct gaatgtactt gtgaacatcc attctgatta ttaaaggtaa ctgccccttg
     7861 aacttgtgat gctattattc tagttgtatc tgatgcacac acactcgtag gcaatattaa
     7921 tatgtggttt cttataccaa ctcttccatc tggtctttta tatccataaa atttcatact
     7981 gatttcctcc taaaatagtc tatatacttt ctataatttt tatatattat aatttttatt
     8041 acactcacta atcaaacagc taaaaaccta tttaaaaact atttgacact aaaatcaatg
     8101 aatacaactt ttataactct ataaatatta ttttatatat tagtcataac ttgtttatcc
     8161 aaatcctctc taacacttct aacattatga atatgtacat gttgaccact ttttatctcc
     8221 tgatttgctt ctcctatatg ttcaccatat ttagtaactt tatcaccttg ggccatatct
     8281 tttatagcca atttatgata aatagttata tcatctagtg ctgtaatcgt ttttactgtt
     8341 ttgtctttta atttaaaact aatttcacta cctttagcta ttgcttcaat agctactgca
     8401 acattatctt tttcatcaat tatcactgca tttatcatta ttcttgctct cctttataaa
     8461 taatataatt ttattaatat cctaaaatct aattaaagaa tttattattt taccaacaac
     8521 tcactgtaat ataaattaag ctgttattgt accatcctca ttttgttttg ggaacttagg
     8581 acatccatac ttcttagccc accaactagt tataagaggt actaatattg cagtgacaac
     8641 tactgcggct gccacttgtg ttgtagctgt tgcaacatat ggttgccatg atgggtcaac
     8701 taatgcaaca gcagcaggta cagcaattgc atttccagca gttgtagcta cagcccatcc
     8761 tgcatatcct ggacgttttc ctataaattt atcacagaat actataaata tacctcctac
     8821 aaatacagat attaatccaa gtaatactcc tgataaacct ccttttagta tacttgcaag
     8881 atttatgcta ctaccaagtg caaaccctac aaaaggtacg agtaaattac accctggctc
     8941 taaaaactct ttcatttttt tgtcaaaatt tcctattatc ataccaacaa gtattggcac
     9001 tattgcagcc aataatgaca acaatggtac atttgcaaga cctgaagtcc caagagctat
     9061 taatgtaaaa aatggtccat catttaaagt aagtaaactc atagttgcac aatctgtttc
     9121 atctccatat gaagtcatca atgaaagata tatactacca ttactatttg taactgcact
     9181 tataacagcc aatgttgtaa gaccaaggac accatccatt ccaaatagct ttcctataag
     9241 tatacctatc aaagcaccta tagcaaattt agaaccaaga agtataccac ctctctttaa
     9301 aacttttggc atttctctaa attgaagctg agttcccaag cataccagtt gaaccccaat
     9361 tatagttgca gacccaacac ttgaaaatac tgctgtcgta aacgaaccta tttgtacaat
     9421 atctgggcaa aaggtattaa tgaaagctgc tataaacatt ggcactatca tcatacctgc
     9481 aggaattttt ttaataaaat ccattatcat aaagttaatc ctcctaaaac atgatattta
     9541 attactctta ttaagttttt agctttaatt ctaacggccg tttcggttcg tatttaagaa
     9601 ctaagttttt aattacgaat ctttgaatta atgatagcat tttcctattt atgagtcaat
     9661 aaaaaaaata aatttttgtc aaaatttttt caaactttct gataatacct tgtatattat
     9721 caaatgactt ccttatatgc aaaaatacag cgtatttaaa attatatagt attc
//
LOCUS       DAYAOY010000132         9672 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYAOY010000132 DAYAOY010000000
VERSION     DAYAOY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.135"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            66..1133
                     /locus_tag="ACGI6B_003567"
     CDS             66..1133
                     /locus_tag="ACGI6B_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFG9752976.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1303..3204
                     /gene="cooS"
                     /locus_tag="ACGI6B_003568"
     CDS             1303..3204
                     /gene="cooS"
                     /locus_tag="ACGI6B_003568"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFG9752977.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3197..3670
                     /locus_tag="ACGI6B_003569"
     CDS             3197..3670
                     /locus_tag="ACGI6B_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9752978.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3645..4871
                     /locus_tag="ACGI6B_003570"
     CDS             3645..4871
                     /locus_tag="ACGI6B_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9752979.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(5010..6197)
                     /locus_tag="ACGI6B_003571"
     CDS             complement(5010..6197)
                     /locus_tag="ACGI6B_003571"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9752980.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6470..7294
                     /locus_tag="ACGI6B_003572"
     CDS             6470..7294
                     /locus_tag="ACGI6B_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9752981.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7423..8688
                     /gene="gluD"
                     /locus_tag="ACGI6B_003573"
     CDS             7423..8688
                     /gene="gluD"
                     /locus_tag="ACGI6B_003573"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HFG9752982.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8901..9491
                     /locus_tag="ACGI6B_003574"
     CDS             8901..9491
                     /locus_tag="ACGI6B_003574"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9752983.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         3760 a         1050 c         1755 g         3107 t
ORIGIN      
        1 aaaaatgtta tactctaaaa aagtatattt tatttaaaaa tttattaata caaaggagat
       61 ttactatgaa aaataaatta gtttacatat gtattatgag tctattgcta tttgtagtag
      121 gatgttctaa taatccaaat aaggaaagta ccattaaaaa agactctaag aataaccaaa
      181 gccaagttaa agaagaaaaa agagaacctg tgaaaaaaga tataactata agtttcgctg
      241 gtgatgtaac tatggggaac tataaaggtt catcatatta tggcagtttt gaccatgaat
      301 ttaaaagaca aggagaagat tacaattatt ttcttaaaaa tgtaaaacct gtatttgaaa
      361 aagatgactt aacagtggtt aatttagaag gtccacttac tacagcgtca aatgcaaaag
      421 aaaaaaaatt tgcatttaaa ggaaagcctg gatatgtaaa tattttaaaa tctggaagtg
      481 tagaggcagt aagtattgca aataatcatt cagaagatta ttttgaagaa ggaatgaaag
      541 atacacagtt tatattggac gagaataaaa taaattattt tggattaggg aaagatgctg
      601 ttgtagatgt caaaggaatt aaagtgggtc tattagggta taatggatta tcaactgaat
      661 ataatgaaaa aaatttaaaa caaatggaag atgatattaa gtccttaaaa aagaaatcgg
      721 atgttgttgt agtgtacttt cattggggga tagaattaga ttattatcca gataaaaaac
      781 aaaaagattt tgctcattat gctatagaca agggagcaga tttagttatg gggtctcacc
      841 cacatgtgat tcaaggaata gaaaagtata aaaataagta tatagcttat agtctaggga
      901 atttttgctt tggaggaaat aagaatcctt ctgatacaga tagttatata tatcaacaaa
      961 cctttacttt ttcagataat aaattaactt caattaaaga gccaaatata ataccaactt
     1021 caataacatc tagtaactcc agaaataatt atcaaccaaa aatattggat ggaagtgaga
     1081 aggatagagt tttaaataaa cttgaaaaaa taagcaaaga tttaaataaa taaaatgtaa
     1141 aaaaaaataa atatatatat ttattttaat aatttatatt ttgtttaggg tatggtaaat
     1201 cgtggtatta aataagtaga ttttaatagt taaattatag tcaattataa taaagctatt
     1261 taatctactt ttttaatata aaaatataag ggggaatttg taatggcttg taacacttgt
     1321 aaaatgtgcg aaagtgcaga caagaaatta gaaagttttg tagcttcaaa ggatgttgaa
     1381 acagcatttc atagaacaga agatcagaaa gttaagtgtg gttttggact tcagggggtt
     1441 tgttgtagac tctgttcaaa tggaccatgt agagtaactc ctaaatcacc aagaggtata
     1501 tgtggagcag atgcagacac tatagtagca agaaactttt taagagcagt agcatcagga
     1561 gcagcttgtt atttacatgt ggtagaaaat acagctaaga atttaaaaaa tgttggaata
     1621 actaaaggtg ttgttaaagg agaaaaaaca ttaaatcaat tagcagagat gtttggaata
     1681 gaaagtgatg aaaagtatga taaatgtata aaggttgcag acaaagttat caacgatttg
     1741 tataaatcaa gagatgataa aatggaatta gtagaaaaaa tagcttatgc acctagagtg
     1801 aaaaagtgga aagagttagg tataatgcct ggtggtgcaa agtcagaagt ttttgatgca
     1861 atagtaaaat catcaacaaa tttaaatagt gacccagtag acatgctagt taattgtttg
     1921 aacttaggta tatcaacagg attatatggt cttacactaa ctaatctttt aaatgatgtt
     1981 atgttgggag aaccagttat aagaatggct cctgtaggat ttaatgtaat agacccagac
     2041 tacataaata taatgataac aggccatcaa cattcaacat ttgcaaattt ccaagataga
     2101 ttaaaagatg aagatgttat aaaattagct caatctgttg gtgcaaaagg ctttaagcta
     2161 gtaggatgta catgtgttgg tcaagattta caattaagag gagaacatta tcaagaagta
     2221 tttgctggac atgcaggtaa caattttaca agtgaggctg ttttatcaac tggagcaata
     2281 gatatagttc tttcagaatt taactgtact ataccagggc ttgaaccaat agcagataaa
     2341 tataaagtaa aaatggtttg tttagatgat gttgctaaaa aagcgaatgc agaatacata
     2401 ggtttagaca gaagtaaatt agatgaactt agtaacactt taatagaaaa agcattggag
     2461 tcttataagg agagaagagg aagtattgaa atagatatac caaaagacca tggatttgaa
     2521 caatctttaa ctggtgtaag tgaaaagaac ctgaaagcgt ttttaggaga ttcatggaaa
     2581 cctcttatta atttaattgc agaaggaaaa attaaaggtg ttgcagctgt tgtaggatgt
     2641 tcaaatatga cagcaggagg acatgatgta aatactgttg aacttactaa agaattgatt
     2701 aaaaaagata taatagtact atctgcagga tgttctactg gaggattaga aaatgttggt
     2761 cttatgtcac ctggagcaga agaattagca ggagaaaact taaaagaagt atgtaagact
     2821 ttaggaatac caccagtatt aaactttgga ccatgtctag ctataggaag actagaaata
     2881 gtagcaacag aattagcagc tgaacttgga attgacctac cacaattacc attagtgctt
     2941 tcagctccac aatggttgga agaacaagca ttagctgatg gagcgtttgg tcttgccctt
     3001 ggcttacctc ttcatttagc tttacctcca tttataacag gaggaaaatt ggtgacagaa
     3061 gtattaacag aaaaattaaa agacttaaca ggaggacatg ttatagtgaa tccagaccct
     3121 aaatcttcag ctaatcaatt agaagaaata ataatagata gaagaaaaaa tttagggtta
     3181 aaggatgtgg aatgcaatgc atagaatttt tataaataaa gatttatgta caggatgtaa
     3241 gagctgtgta ctagcatgta tgctaaaaca caataaagat tatgacatgt acactcttga
     3301 tttagaaaat atagataatg atagtagagg gcatatagaa ttagatagta aacataataa
     3361 tccagttcca attctttgta gacactgtga tgaacctgaa tgtgtattag catgtatgag
     3421 tggtgcaatg cataaagata gtgaaagtgg aatcgtttca tatgatgaag agaaatgtgg
     3481 ttcatgttat atgtgtgtaa tgtcttgtcc atatggatta ttaaagccag atgatagaag
     3541 caagcagaac atacttaaat gtgatttatg taaagatgag gagtatccta gatgtgtagc
     3601 taattgccca agtggagcaa ttgaattaca gaaggaggaa aatgatgaat tatgtagtgt
     3661 taggggctag tgcagctggt ataaatgcag taaaaacatt aagagaatta gataaagatt
     3721 caaatatagt agtaatttct aaagatgaaa acgtatattc aagatgtatg ttgcatcacg
     3781 ttatatctga acacagaatt ttaaaacaaa taaattttgt tgatgaagat tttatggagc
     3841 aaaataatgt aaaatggatt gcaggtaaga ctgttaaagg tatagacata aataaaaagg
     3901 tagttcagac agaagacatt actgtaaatt atgataaact tttaatagct acaggtgcat
     3961 cttctgctat tcctcctata aaaaatttaa gagatggaaa ttttgtatat tctgtgagaa
     4021 acatagatga tatttataaa ataaaagaaa aagcacaaaa ttctaaaaat gtagttataa
     4081 taggtgctgg tttagttggt atagatgcat tagtgggatt atttaaatat gagcagttaa
     4141 atatttctgt agcttttatg gaaaagtaca ttttagatag acaattggat gaatatacag
     4201 catcagtgta tgaaaataag ttcaaagaaa aaggtgtaaa attttaccca agtgcatcaa
     4261 tacaagaaat tgtattggat aacagtaaaa atgttacagg tgttgcattt tcaaatggtg
     4321 aagttttaga tgcagacatg gtaattgttg ctacaggggt taaaccaaat gctgactttt
     4381 tagatggaac aggaatagaa tatgatagag gtataataat agatgatatg tgccaaacaa
     4441 cacaaaaaga catatatgca gcaggagatg ttgtaggtaa aaatgctatt tggcctctgg
     4501 cagtaaagca aggaattgta gctgcctata atatggttgg aaaagataag aagatagaag
     4561 atgaatttgc ttttaaaaac agtatgaatt tcatggatat accaactatt tctataggaa
     4621 tgaatacacc tgtagatgat agctataaag tgctaactag acatggaatt aatgattata
     4681 aaaagtttgt ttttaaagat aatgtaatct atggagcagt tatacaagga gatatttcat
     4741 atgtaggtgt attaacttat ttaataaaaa ataaagttga aatatatgat ttagaaaata
     4801 ggatatttga tatatgttat gcagactttt ttaatataaa agaaaatgga gaattttgtt
     4861 atagtgtata gtatatacgt ggagtaaggt ttataaatac agtaaatcaa taaatagagt
     4921 tattctaaaa taagaaaaat gacaaatctt atttagaaat agctctattt tttgtaatta
     4981 aatttaaatt atattcaatt tgtaaaatat tattttgaac aattatacat atactctcta
     5041 gttagaggta aatcattttt tataccttta gtgaaaagta tttgatgtat atctataata
     5101 ccagtgttga aagcagcagc acaagaacaa aggtataaat cccacattct tataaactta
     5161 tcatcaaaca tttctttaat ctttggaaga ttctcagaga aatttttttg ccagtatagt
     5221 aaggttttta cataatgtaa tcttagactt tctacgtcta tagtatgata tttgtaatca
     5281 ctacaaatat gtatcatttc tcttaggcta ggaataactc ctcctgggaa aatatatttt
     5341 tttatccaag ggtctccata agattcctgc aatccactta tatagtgaag taaaaacaat
     5401 ccaccatctt ttaaaacagc atttacgttt ttaatgaaaa gttcatagtt ttctctacca
     5461 acatgttcaa gcatcccaac gcttacaatt ctatcaaaca taaggtctga tttttttaat
     5521 tctctatagt ccataagtct tacttcaaga tagtcttgaa ggttctcttc tttaattctt
     5581 gcgttaaatt ccttaaattg ctcttcgcta agtgtaattc caacgccgtg aattttatat
     5641 ttttttgcag cttctattag taaaaatccc caaccacaac caatatcgag aaggctcata
     5701 ccttcctcta aatttagttt agttaatatg tggtgaactt tattgacttg agcatcataa
     5761 agagtatcat tatcatttct aaagtatgca caagaataac taagtgtctt atctaaccat
     5821 aagctataaa aatcgttgcc aatgtcatag tgggcatgta tttctttctt ttgatgtctc
     5881 attgaatttg atgagtgcaa tagatgtttt acagctgaat tatcaatatt gaatttgtct
     5941 atttgtttta aaagtagatt taaagttaag aataaatctc cctcaacttc aatgtctcta
     6001 tttatgtagg cttctccaag tgctaaagat gtacttttta atagttcaga cttgctaata
     6061 tcattattta ctataacttt gaactgaggg gagccctctc caattagaaa ttcttcctca
     6121 tctttaagcc taactagaaa tggtgtttca ccaaatttct ctaaatagtc ttttaaaaaa
     6181 ttttttgtaa tattcatatt accagctcct tctaatgtaa atccagtaag tttccctgct
     6241 taaaatatat tatagtcctt tttttaaaaa agtaaaattt tttcaataaa ttttttattt
     6301 aaaggaaatt tattttttat aaagaaattt catgttgtag tatatttttt gcttataaat
     6361 tttggtactg gatataatat taaactgtta atgatataat aaatttaatc tataaagtaa
     6421 ataaaatttt gtaataaata gttaaagcta ttttataagg agatatatga tggaagtata
     6481 tgctttggtt gggtcaagtg gcacagggaa gagctttaag gctttagagt tcgcatatga
     6541 aaatgatata gaatatataa ttgatgatgg gattttaata tacaaaaaca aggttttagc
     6601 tgggatatcg gcaaaacaag caaatactac tatagaagca gttaaaaggg ctatttttta
     6661 taatctggag catagacaag aagttagaga agcaattaaa aaagaaaata taagaaaaat
     6721 tcttattatt gggacatcaa aaaaaatggt taatcaaatt gtagagagat tgtcaattgg
     6781 aagggtttgc aagtttataa atatacaaga tataagtact aaatatgaaa tagaaatagc
     6841 aaaacaagct agaaaagaag gaaatcatgt aattcctgtt ccagcagttg agataaaaag
     6901 tatggcaagt ggtcttagta taaattcttt gaagagactt tttagaaaag gaagtaatag
     6961 gaatatggta gttttagaaa aaacaattat aagaccaact tttagttata taggaaagtt
     7021 ttatattagt gctgatgtaa ttaaacagat tattgaatat gaagtataca actttggaag
     7081 cattgataga ataaataaaa tcaatataga gaattgcaat aattttatga atatttttgt
     7141 aagtataaat atcaatgatt tagaaactat aaaaaaaatt gaagacatac aacagacaat
     7201 aaaaaagtct attgagaaaa tgactttagt taatgtacaa aaaatagata ttaacattca
     7261 taaaattaaa aaaataaact tcctccaatg ttaaaaaata aactgagaaa atgatatact
     7321 aatttgcaat aaagtgtgct ataattaaac tgtaaattta ggaaatggtt ttttctaaat
     7381 ggagtataca taattataac gtatttataa gggggctttc aaatgtcagg aaaagatgta
     7441 aatgtcttcg agatggcgca atctcaagta aaaaatgcat gtgataaatt aggtatggaa
     7501 ccagcagttt atgaattatt aaaagaacct atgagagtta tagaggtttc aattccagta
     7561 aaaatggatg atggttctat aaaaactttt aaaggattta gatcacaaca taatgatgca
     7621 gtagggccaa caaaaggtgg aataagattc catcaaaatg tttcaagaga cgaagtaaaa
     7681 gctttatcta tatggatgac tttcaagtgt tctgtaacag gtataccata tggtggaggt
     7741 aaaggtggaa taatagtaga tccatcaact ttatctcaag gtgaattaga aagattaagt
     7801 agaggataca tagatggaat atataaatta ataggtgaga aagttgacgt tcctgcacca
     7861 gatgtaaata ctaatggtca aataatgtct tggatggttg atgagtacaa taaattaact
     7921 ggacaaagtt ctataggtgt tataactggt aagccagttg aatttggtgg ttctttagga
     7981 agaacagctg ctactggatt tggtgttgct gttactgcaa gagaagctgc tgctaaatta
     8041 ggaatagata tgaaaaaagc taaaatagct gttcaaggta taggaaacgt tggttcttat
     8101 acagttctta actgtgaaaa acttggtggt actgttgtag ctatggctga atggtgtaaa
     8161 tcagaaggtt cttatgctat atacaatgaa aatggtttag atggtcaagc tatgttagat
     8221 tatatgaaag aacatggtaa cttattaaac ttcccaggag ctaagagaat atctttagaa
     8281 gagttctggg cttcagatgt tgatatagta ataccagctg cattagaaaa ctctataact
     8341 aaagaagttg ctgaatctat aaaagctaaa ttagtttgtg aggctgctaa tggaccaact
     8401 actccagagg ctgatgaagt atttgctgaa agaggaatag ttcttactcc agatatatta
     8461 actaatgctg gtggagttac agtttcttac ttcgagtggg tacaaaactt atatggatac
     8521 tactggtcag aagaagaagt agagcaaaaa gaagaaatag ctatggttaa agcatttgaa
     8581 tctatttgga aaatcaaaga agagtacaat gttacaatga gagaagctgc ttacatgcat
     8641 tcaattaaga aagttgctga agctatgaaa ttaagaggat ggtactaaga tttactctta
     8701 ataaataata ggatataatt atttaagata taaaggggct gtacatagta cagcccttaa
     8761 attatgtggc ttacaatgaa gtaagctatt tttgttttaa cttataaaaa acttgataaa
     8821 ttatgtaatt tagaatgtat tttttaccac cacatgatta caaattagaa gagttagatt
     8881 agatttttag acatgaactt atgcatttta aaagcaaaga ccttaattta aagtatctag
     8941 tattattttt aaaaatagta tattggttta atccatttat atatataatg gaaaaatata
     9001 tggatttgga ttgtgaactt tattgtgatg agagagtttt aaaaaattgt actgtacaac
     9061 aaaagcaaga atatgctcta actattgtaa attcaatgaa aagaagtaca aatttttcaa
     9121 ataaatttgt agctggatta aataaacaat ctgatattag aaaaagagta ataaacatgt
     9181 tcagtgaaaa acataaaaat ggtatcttaa gggctttgat tataagcttg ttttcttcta
     9241 ttacattttt taaattcgat tcaactagta ttattgaaga gaatgatttc ttagagaggt
     9301 caagatatac gtttgaagga aggcaagaag acatgtttgt aacaatagat tttatatatg
     9361 cagatgtacc tataaaactt agaaaagagc atgaagaaaa ctgtagagtc aatggaatag
     9421 taccaaaaga ttcagattca atagaaatta ttcctagatt ttataaagat tgtatggatt
     9481 taaaaaaata agttagactt aatattgatg ttgatattat gaatttttta tatttaagca
     9541 attgaaaatt ttattataga ttcaatttta tatgtgttta agtatgaaat ttatagatag
     9601 ttgaaataat taaatatgat tgatgcatag gaatcattac taagctataa ttaattataa
     9661 gggagtttaa at
//
LOCUS       DAYAOY010000133         9456 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYAOY010000133 DAYAOY010000000
VERSION     DAYAOY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.136"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     misc_binding    142..353
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            516..1805
                     /locus_tag="ACGI6B_003575"
     CDS             516..1805
                     /locus_tag="ACGI6B_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFG9752984.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1859..2938
                     /locus_tag="ACGI6B_003576"
     CDS             1859..2938
                     /locus_tag="ACGI6B_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HFG9752985.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2959..4503
                     /locus_tag="ACGI6B_003577"
     CDS             2959..4503
                     /locus_tag="ACGI6B_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HFG9752986.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4666..5208)
                     /locus_tag="ACGI6B_003578"
     CDS             complement(4666..5208)
                     /locus_tag="ACGI6B_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFG9752987.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5356..5640)
                     /locus_tag="ACGI6B_003579"
     CDS             complement(5356..5640)
                     /locus_tag="ACGI6B_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9752988.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5703..6467)
                     /locus_tag="ACGI6B_003580"
     CDS             complement(5703..6467)
                     /locus_tag="ACGI6B_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFG9752989.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6749..7015)
                     /locus_tag="ACGI6B_003581"
     CDS             complement(6749..7015)
                     /locus_tag="ACGI6B_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9752990.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7057..7866)
                     /locus_tag="ACGI6B_003582"
     CDS             complement(7057..7866)
                     /locus_tag="ACGI6B_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9752991.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            8179..8460
                     /locus_tag="ACGI6B_003583"
     CDS             8179..8460
                     /locus_tag="ACGI6B_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752992.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            8724..8978
                     /locus_tag="ACGI6B_003584"
     CDS             8724..8978
                     /locus_tag="ACGI6B_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752993.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            9023..9346
                     /locus_tag="ACGI6B_003585"
     CDS             9023..9346
                     /locus_tag="ACGI6B_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFG9752994.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT         3425 a         1110 c         1522 g         3399 t
ORIGIN      
        1 aaaaaataca ttatataaat atagtaatag tatgaaaatt tacatataaa actaaaaaat
       61 gcaagtaatt gactttttaa gttgacaaaa tattgcatat atattatgat ataaatcata
      121 aattaactac aaatcaatat tatatattcg tagaagaaga aagtagcata aaggaatttt
      181 acagagagtt agatttggct gagaactaat attggaattt atgtgaaaag gactttggag
      241 aatactattg taattatgtt agaatagtgg tttaattcgc cttaagaaaa tgagtgagta
      301 aaattaattt ttactaataa gagtggtaac acggatttat attcgtctct tggatttttc
      361 caagaggcgt tttttattat aaacttattt cagaaaattt aaaactatat attaaatgaa
      421 aattataaaa atatgatagt taataaattt ataaattaga aaattgtaaa atatgctaag
      481 gtcagtagtt ttacgattta aatggaggga tgaaaatgga agaagaaaaa attaactatg
      541 ctctggaaca gattccagag aatcaaaaac gtggttgggt agctatgttt tcagttttag
      601 tagcaatagg tgtggattta tcatctgtta ttttaggcgc tgagttggca cagagtatgc
      661 caatgaaaca agcaatattg tcagtaattg ttggttcatt tttttctgca attttgtata
      721 ctacatgctc tttggttggt tcatctacaa gtttatctac atctatgata acaaagtatg
      781 tatttggaga agctggagca aaaatatttt ctctagttat aggggtttct ctattaggtt
      841 ggtttggagt tcaagtaggt ttctttgctc aaaatgcaca gataataata aaagatatat
      901 ttaatttaga tgtcagtatg cagattttaa gcctaatagg tggtctcttg atgatgagta
      961 ctgcaattta tggatataag gcaatggaga agttgagtgt gtattcagta ccatttttat
     1021 tagttcttat gatgctaact atattcttag cttttagagc aaatggaatt tcagttgatg
     1081 ataatatgaa atctactatg acatttgcag gtggagtttc tctgtcaatg agtataataa
     1141 tagttggtgc aattgtatct cctgatataa gtcgttgggc taaaagtaga agagactgtg
     1201 ctctttcaag ctttttggga atacaatttg gaaatgcttt tatgataata gtttcgatag
     1261 tattagtaaa atgtatggga acttcagata ttatgagaat ttttattaca ttaggaattg
     1321 ctataccagg tataatagta cttacattag cacaatggac tacaaataca agtaatgtat
     1381 attcagcatc attaagtata gctcttgtac ttaagaaggc tcctgagaaa gtattaacta
     1441 tagtccttgg tattatagca acattattgg ctgtatttgg aatatatgaa ggttttatag
     1501 gatttttaaa tttacttgga attgtaattg cacctgttgg tggtgtatat acagcagaat
     1561 attacatagt aaagcaagaa cttaaaggct ttgataaagg agtattatat aaaccaatag
     1621 ttaaacgttc aatagtgtct tggataattg gtatattaat aacttacctt tcaacatatg
     1681 gatttataac tcttactact atagcacctt tagatggttt tatagcaggg tttgtagtac
     1741 aatctattat tggaaaagtg ctttgttcaa caaaaaataa gcaagaaata gacaaagcag
     1801 tataaagaaa tttgtttata aagtataaaa taaattagaa atatgaggag aaagtaacat
     1861 gagattttta acagaagaaa gtgtagataa aatagcagtt ggtgcagcag tgcttggaac
     1921 tggaggtgga ggtgacccat atgtaggtaa attggttgca aaacaagcca ttaaaaagta
     1981 tggtcctgta aaagtaataa gtttagatga acttgatgat gatgctttgg tagttccagt
     2041 atctggtatg ggttctcctg taattacaat agaaaaatta ctttcagaag tagagttaac
     2101 taccccttta gaaataatgg agaaattatt aaatagaaaa gtggatgtta taattcctat
     2161 agaaatagga gggataaact cacttatgcc tatagcagtt gctgctaaaa aaggacttcc
     2221 tatcttagat gcagattcta tgggtagagc tttccctgaa gctcaaatgg taacttttta
     2281 tcttgaagga tatgaggcat ctcctgccgt aatgtctgat gaaaaaggta attctgccat
     2341 cctttatcct gtggatggaa tttggtcaga aagattagct agaactttaa ctgtagaaat
     2401 gggaggaagt tcttctatta gtgactataa tttaagtgga gctcaagtta aaaaagctgc
     2461 tataggtaat actcttacaa tagctgaaac tataggaggg tttttacttg aaaataaatc
     2521 tgattcaaaa ggtgctgttg agaatatact aaaagaattg tgtggataca agttatttga
     2581 aggaaaagta attgatatta agagagagtt gaaaggtgga tttactagag gacatgcctt
     2641 ttttgctggg attaatgagt atgatggaga gtattctata ttattccaaa atgagaattt
     2701 aattgccaaa aaaggtgata caccactttg tattacacca gatttaatag cagtattaga
     2761 tttagaaact ggatttccaa taactacaga gagaataaaa tatggctctc gtgttatggt
     2821 agtagctttt ccttgtaatg aaaaatggag aacagaaaaa ggaattgaga cagttggacc
     2881 tggatacttt ggatatgatg tggaatataa aacagtagaa gaattacaag gaaaataaaa
     2941 gtttaggagg aaattttgat gtatcgtgta ggaattgacg ttggaggaac aaatacagat
     3001 gcttgtatac tggatgggga attaaaagta attcattcag ttaaggtggc aactacaaaa
     3061 gatgttgaaa ctggtgttta caatgcctta aaaaaagtta tagatgaaag taagattgac
     3121 cattcattaa taaaatatgc tatgcttggt acaactcatt gtacaaatgc tattgtagaa
     3181 agaaaaagac ttaataatgt ggctatgata agaataggaa agccggccac tactgccatt
     3241 cttccattta tagattggcc agaagactta agagaaaaaa tagaattaga ttcaatattg
     3301 gtatctggtg gatatgagtt tgatggcaga aaaataaatg aactttgtaa agatgaagtt
     3361 gtagagtttt gcaatagaat aaaaggcaaa gtggaaagtg ttgcaataag tggagtattt
     3421 gctccagtaa ataaacaaca agaagaagaa gttgcaaaat gggttagaga aattttaggg
     3481 gatattccta tatctttatc aaatgaaata ggtaatatag gtatattaga aagagaaaat
     3541 ggagctattt taaattctgc attgtctcaa gtaggtaaag ctgtatcttt aggatttgaa
     3601 aaagcactta aagatttagg tatagatgct caaatatatt tcagtcaaaa tgatggaaca
     3661 ttaatgaatt tagagtatac aatgaaatac ccaattttta caattggatg tggaataaca
     3721 aattctataa gaggagcttc cttcttatca aaagtaaaaa atgcaatagt attagatgtt
     3781 ggtggaacta caagcgactt gggtgtacta tataatggat tccctagaga atcatctatt
     3841 ccagttacaa ttggaggagt acatacaaac tttagaatgc cagatgtttt aactattggt
     3901 cttgctggtg gtacatgtgt aaaaggcgaa aaagataata taaagatagg tcctgaaagt
     3961 gtgggatata gaataactga agatgcaata atatttggtg gagacactct aactttatca
     4021 gatgttgtta caaaattaga tatggcgttt gaagatagaa aaagcaatgt tgaaaactta
     4081 gataaagagt attgtaatga ggtatataag aaggttattt taaaattaga agatgctgta
     4141 gaccaaatga agactagtca agatgatgtt gaattggttt taactggtgg aggaagtata
     4201 attgttcctg aaaaactaaa aggcataagc aatgttataa gacctcctca ttatactgct
     4261 gcaaatgcta ttggtgcagc tttaggacaa ataagtggag atgtagaaaa agtatacagt
     4321 ttagataaaa tgtcgagaga agatgccata gaagatgcaa aaaaagaggc tgtaaataaa
     4381 gctctaaaag ctggtgctaa agaagatact atagaagtat taaatataga agatgttcca
     4441 cttgcatacc ttcctggtaa tgcacttctt ataaaagtaa aagttgttgg tgatttaatt
     4501 taaagaataa atataagtat ttccagaatt atataaatat ttctggactc atttttaaac
     4561 ttattttatc aataaaaaca gcataacttt tgactatatt cttataagtt tccgaacagc
     4621 cgatatatac ctattttttg aggtttatcg gctgtttgtt atcttttatc gcattccaac
     4681 tatttttctt accgtatttg ctgtacgaat tgtaatctta tcacttacat ttgaactggc
     4741 agtctttgac caccaatttg tctttttata atctttacga ctaaaagacc aaaagatagc
     4801 atttttataa tgatatactt tttcatactc ttctttagga tttccaacct catcataaaa
     4861 ctcgtcataa gataagtcag gaaacataaa aataagatta tcatatattt ctttgttgtt
     4921 atctccccac caattaggag cattatttag tatatctttt aactcttctt gtgaaataat
     4981 aaaaactggt atttccaatt caaactgttt ttttatcatt aatcttattg tatttgagat
     5041 aaccttgctg tcatctacat cacttaaaaa gacaacatta ccactattaa ggtatgtaga
     5101 aacttctgaa taaccaagtt ctacaaagct tgcttttaat tcagacataa ttatcttgtt
     5161 cttaccactt atattgatac ctcttaataa tgcgatataa cttttcatat aataatttca
     5221 cctctaaaca ttcacatttt tctatgtagt acaaacttga atttgcatct tttataattc
     5281 tttcaaaact tcttcatatg taattttttt aatataaaaa aatcggtgct taatttgtac
     5341 cgatttttgt ttttcctagt aaagaactcg tttttttgct tctgctatag ctctagcagc
     5401 actttttgct tgcattcttt tttcttcttc ttttttaaat tcatttattg tttcatttag
     5461 attcttatca tgttcatttt tttctttatc ttcgttttct ctgtatacta atatataatc
     5521 tattattgat tcgtttatta tctgagatat acttttattt tcattaattc ctattctctt
     5581 aacttcctta agtagagtct catcaatatg aaccgttgtt tgctttgtat tttttgccac
     5641 cttaatcacc tcttaaaaaa aattctaaca ttaaaatttt ttttagtcaa taaattttaa
     5701 tattaaaatt ctttaaatgt tttatttgat tttctaaaat tttctattgt atttatcatc
     5761 ttatttttta tattttcacc tagtacagaa gactttagtc taggattttc gatatggtca
     5821 taaattggtt ctaaaaattc aacttcgaca ggaacagagt taattttacc aataggttgg
     5881 tatccttcat aagtatcttt tgaatttttt attacgagtg gaacaatagg gcattttgct
     5941 ttatatgcta tctttaaagc tccacttcta aactccgaaa ccaatgaatt tggttcttta
     6001 atccatgtta aatctccttc tggaaacact gccatacttt gacctgtaag tatattttga
     6061 gaggcttggg ctatactctt tataccctca cgattgtttt ttctgtttac atatacacat
     6121 cttagtaatt ttgcccattc tttaaatata ggtatatttc tccaaactgg ttcatctgca
     6181 attacacatc caattggtct ttctacactt gctgttagta tgaagctgtc aagcatacta
     6241 gaatgattta caacaaatag aagtggttct tttggcagtg tttccttacc aattatatta
     6301 agctctatat tcactatatc taaagatttt ttagcttgtt tttgcataaa ttcaaatttt
     6361 tcctttaagc taaatttatc aggattcttt ttaattttaa taagcctagg tatagaagat
     6421 gtaaaaccta taagttgata tgtagcaaat tttaagtaat tattcataaa attatctcct
     6481 taaaataaaa ttatttatta ctgtaaagat tttaacataa aataaaattt aagtctattt
     6541 taattcaatt ttgtaacata tattttaaat agacagagat tgagatttta tatttccaat
     6601 tttagtaatc agattttatc aataaattac tattctatta atttaattaa ataaccttta
     6661 aatttaatta taaaaactgt tgcagaaatt tctacaacag tttaaaaaat ttaccttaca
     6721 aaaacttatt ttataacaag agagtatatt ataaggctag tacagcccat tttttgcact
     6781 gagaaattct tttgccatca tcaccttgtt ctttgtcata tgcaaattga ttaagcattt
     6841 cacatggaaa tgtatcacat agaccacaat gtgtaaggtt tttatcttca caacaagatt
     6901 ttacaggaca ttgttcaccc caaaatggtt tctcaatctc tgtacaacct ttacaattga
     6961 ctttttcctt gtatccacat tcataacata aaattccaca tcttgattct atcattttaa
     7021 gtcactcctt aaatataaat tttatatgat tgtatatcaa aatttatact tttcattgta
     7081 aaaatcggac aaatttttta tataggttgt tggattaaca ccacaaatag atttaaaatc
     7141 atttataaaa tgagattggt cgtaataacc aatatcttgt gctaaagttg caaatggtat
     7201 tttattattt tgtatttctt gtaatacaag gtttatacga agtaatctaa ggtatgattt
     7261 aacactcata ccaagagaat tattaaaaat tctatttaaa tgtcgttcac tataacaact
     7321 aatttgtgaa atgtttttaa cagacataat agagttttgt tttctagcat tttcaagaat
     7381 tggtatagtc atatctacta tatttgattt caataagtat gataagaata acatatctaa
     7441 ttgttctact aattccttta tagtacttgt tctttcaaaa atggaattaa cctctaagct
     7501 aaataatgtg ttaaaatctt caagtgaaat ttttaaatct gtagattctc tttgagaaag
     7561 acctgtcaaa taatatactc cacctgggcg aaactcaacg aatactctaa aaagtacatt
     7621 ttctatatca ttttttacaa tagtagtttt tgttgtggct ccccagaatg cgctttctat
     7681 accttttttg tcaaatttaa atatcataca tccactagca tcaggtatta atgtaagatt
     7741 ttctttaata cttgtatctg gaatagatat tgtatagtgt gctatatact gtcttaacaa
     7801 tttatgtggc aaaatatatg tatactgttt gcaggtattt aaaatgttat tgattatttt
     7861 attcataagt atatcctcct gatatataaa aaataagtat actaatttat tttttatata
     7921 ataaaccagt tttgttttaa ttgaaatatt ctaataagat attttgttaa atttagatac
     7981 aataattgta aagtaaaatc tcaaaatata caattattat aaatcataga tatttattaa
     8041 ataaatatat taaagaagaa ggaatataaa acaagattag aaaataatag tgttactttc
     8101 ataataaata catataaaga tatttttaag aataaaattt attattaaat aaaaaataaa
     8161 ggagagtgat ttaatagcat ggcatcaaaa caaataccaa gaaacaattt agaaaagttt
     8221 ttaagaataa gagtcttatc acatacaggc gataaaatac atataaagtt acctgtaaat
     8281 tttgcaaaga gaatgattga gaataatgct ttggatttat ttaatggaaa agatgatgta
     8341 gtagatggta aaaaagttac tgaaatatta gttaaggcat ttgactatga tttaactgga
     8401 gaagtagttc acttggagag aaaaaatgga gacattatta aagtttcaat tgtacaatag
     8461 aattatttta tatgaatttt atttttgaat agttataaat aattagaaat ttgtaaaaaa
     8521 gtataatata ttattagtaa tggtacagaa tacacattta taatttatag atgtgtattt
     8581 tttgtttaaa aaatttaaat atataaaact ataaaggtta caattttgga aatagatgta
     8641 aatatatgtt gtattgtata ataagagtaa tataaaatct agtaaaaata atcaaataaa
     8701 tttagcttat gagagggata actatggaaa ataaggattt taaattaaca atatgtatat
     8761 ttgcttttgt aattggagta gtagtattat tctgttgtat aggtttaggt gaatttagtg
     8821 cagagcatat tattcaacaa aatggtggaa tcatggatac tgaccaatat aatatttatc
     8881 tagaccaatc tatttctcaa tatagaaatt ttggttctat attagctctg ttaggaggaa
     8941 tagggatatt gtttgataaa tatatcagaa aatactagag attatgtttt gtctaaaata
     9001 aattcttatg aggagataaa aaatggcaac agaaatattt actgtaaaaa ttacaccctt
     9061 agaggcacaa aaaatgataa aagaaaatct tgatgcagat ttagttttta gtgattctta
     9121 taatttagga gatgataaat ttatactaat tacaactttt gagaagtatt atgcaagaaa
     9181 taatagtgat gcaggattaa tagtagttta tgaaaatacc acaggaaaaa cagttattaa
     9241 agctacttca acaggcagtg caattggaat tttaaaaatt gattggggtg caggtggaaa
     9301 tttacttaaa agagtaaaaa acatacttgc tgattatatt atttaattat tttatctaac
     9361 tgataatatg tcaagaaaat ctcgtaaaag aggttaagaa catttttata atatatagat
     9421 aaataagaat ttgaaggtaa agtttttaag gtataa
//
LOCUS       DAYAOY010000134         9421 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAYAOY010000134 DAYAOY010000000
VERSION     DAYAOY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.137"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1063
                     /locus_tag="ACGI6B_003586"
     CDS             <1..1063
                     /locus_tag="ACGI6B_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752995.1"
                     /translation="NINFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLID
                     VSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFT
                     RTRYKVDKSTDDYYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPEN
                     KKIKTIKSINLSNYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHL
                     LEYNLEENKESIMELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILD
                     KSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIV
                     YEGELQFNSSNSMLEFEIK"
     gene            1072..1884
                     /locus_tag="ACGI6B_003587"
     CDS             1072..1884
                     /locus_tag="ACGI6B_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752996.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1895..3163
                     /locus_tag="ACGI6B_003588"
     CDS             1895..3163
                     /locus_tag="ACGI6B_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9752997.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIIDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            3529..4710
                     /locus_tag="ACGI6B_003589"
     CDS             3529..4710
                     /locus_tag="ACGI6B_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9752998.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(4852..5769)
                     /locus_tag="ACGI6B_003590"
     CDS             complement(4852..5769)
                     /locus_tag="ACGI6B_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HFG9752999.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            6022..6816
                     /locus_tag="ACGI6B_003591"
     CDS             6022..6816
                     /locus_tag="ACGI6B_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753000.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            6817..8877
                     /locus_tag="ACGI6B_003592"
     CDS             6817..8877
                     /locus_tag="ACGI6B_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HFG9753001.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         3793 a          923 c         1467 g         3238 t
ORIGIN      
        1 aaacataaat tttggatatg ctttgaatgg atatataaat tcatattata caataaatgg
       61 aagcaacaaa accaaaaaga ctgaattaat aaaactacaa aaggatagta agaacacaaa
      121 taatttaatt gatgtatcta gtggttcaga tttaattgct catgttggat ttgaaatgaa
      181 tgaggagtca aaagatataa actttgtgat agatgagaaa aaagaagatt tagaaaaagg
      241 tgtatttcca tcagaaggta gtgataaaga aattgaatat gaaaaattag atatgaataa
      301 ttttacaagg acaagatata aagtagataa aagtacagat gattattatg caaagatact
      361 agcatcaaaa agatataaaa aatacttata tgtatctgta ctttatgcta caagtgattc
      421 atcagaaaat aacaaagaat attcaagtac tatagatata ataaaaatta atccagaaaa
      481 taaaaaaatc aagactataa aaagtataaa tttaagtaat tatttttccg aaaaatacca
      541 aaacatagag aacataggtg taagttatgc tagcaaatat gaggataaga tgtatatttt
      601 agtaaatgta ctaaatacaa acataggtga taatagtaat tctaaatttc atttacttga
      661 gtataatctt gaagaaaata aggaaagcat aatggaatta aaaactgata gcaaaatggc
      721 tttagcaaaa gcaaagttag aagctaataa attatatatg gtaatgtcga atgattatga
      781 taatagttca attacagagt taaatttact tacctataat atattagata aaagcttaga
      841 tgaagataaa tatgttttag ataatagaga aaaaagagga attagaagta ttgaaattga
      901 ttcaaataaa atcgttgcat atacatcaag gctaaactct atattatctt gggatgataa
      961 atttatgcta tatataataa ataaaaattc taaggaaatt gtatatgaag gagaattaca
     1021 atttaacagt tcaaatagta tgttagaatt tgaaataaag taggtgagtt gatgaaagat
     1081 ttaataagat tattcgaatt tgaattaaaa agaaatataa agaattatat atttataata
     1141 atttgttgtt gttcttttgt aattttaaac atagtaaaaa atttaaatga ctataattac
     1201 ataatagaaa atgcagttaa aagtgaacag ttaacaaaaa tcggaaatac gatagaaacg
     1261 gtaaatgtta caggtttctc aagctttaga aatataatgg gttctacaga atcgtggttt
     1321 atgtttggta taattgcatg tatttgctat gcttttttta tttggtatag agactttaat
     1381 ggtagaagta aatctatata tacacttata atgctaccta aaaatagaat caatatttat
     1441 atatctaaat tattaaatat attatttcta gtgtatagct atacagtagt acttacaata
     1501 tctttgttta ttgcaagtaa gttgttgcct agacatatgc ttggtaatgt cacaaactat
     1561 ggttttgtac aagaaacaat atatgagtta aaaatgttac ttccatatag ctttgaaata
     1621 ctatttgtag agtatatatt tttattgata ggatttgtat ctgtagtatt tacatcaatt
     1681 cttataaata aatctattta taaagtaagc actttaataa gttttctatt tttagttata
     1741 gaaattttag tgtttataat ttctatagaa tttattatac cttacgaata cagtgatgta
     1801 tttactgttc tatattcaag tataaacata tttgtatgta gtcttatatc taataaactt
     1861 ttgaagcaaa agattgactt ttaggaggga tattatgaaa gcaaaaaata taggaaaata
     1921 tacaatatgt tttataatag tatgtttttt aatgattact atgaccttaa tattgagtta
     1981 ttttaataag gataaactat ctgtaaaaac tttaaaaggc aatgtaaaag atatagggag
     2041 agtaagtgta gtatatacac ctaatataag caaattttct tatgaggagg taattataag
     2101 taaaaatgga gtaagaaata atacaaaccc aaaatcaaga attagaaatg attatttaaa
     2161 tattgacaaa aaatacagtg actttgttaa tgataaagga aaagaattta tatataaaga
     2221 tgatttgaat ataggagaag cgtatacctc tgttaagtct gatttagtac aaggaatgaa
     2281 tgctttaaat gattgtataa aggtttcaaa taaaaattta aaaagtgggg tagaagaaaa
     2341 ttttgatatt aatttaaata agtctgattt taaagaaaat agttggaaaa ccttatttaa
     2401 caaaatatat aaaggtaatt tatatatggt aattagtgaa actaatattt caggttctaa
     2461 agtcaataat atttgtatat taaagataga tataaaaaat aagtcttata aattattaaa
     2521 tagatttaat ttaggattac agaatggaga acaacaaaat atcaatgaaa ttaaattttc
     2581 tattggaagt aaattatact tagaaatagt aaataataaa agtaaaaatg gatatattga
     2641 aggacatgat agttactcta actttattat atatgatata gaagataaca cttttaatat
     2701 aagtaataaa tttatggata atctaaacaa agaaaagaat gcaaatccaa aggagattgg
     2761 cttattaagt aaaactaagc tagattatat ggtaaaaaac aataaattga atcttatagt
     2821 tacggatatg agtaattcca taactaatct tgtttatgat attaattcta atgagattaa
     2881 attagaagat tatgagatac ttgatttaaa agtagattat aatgaagcat catctggagt
     2941 taggaaagtt aagctactgg atgataagat atattctatt tcaggtatta agaacagtat
     3001 atcaggagaa ttaaaaggat ttaaagggat tattgatgta gtcggaacta agccagtaaa
     3061 atttcaagta tttgatatga aacttaaaaa gaatatttat gaagctgagc taataaatgg
     3121 agatataagt ttacaagaaa agttattttt tgttgcaaat taagtgttag aattaatttt
     3181 agaactttaa acttttatat gtatcacaga taagtataag taagggagtg tctcaaaata
     3241 aactttttag ctcatgagac acttttttta tatgaaatca aatatatttt cattgtttag
     3301 atttattttg ataatgaaaa aaagattatc tttattttaa gaaagacatt tatttataat
     3361 tatatacaaa aatatttttt aatgtttttg tgtataatta taacaattta atttatatta
     3421 atgcacttaa atttgaaata gaatgcttat agtggtaaaa ttaattatta gaatattaga
     3481 aataataaat aaataataca ttgtacttaa aagggggaga aatttaagat gaacaaaaaa
     3541 tatattaaag cagggaattt aattgatgga accggaaata gtattatgca aaataagggt
     3601 ataattattg atggagatac tatagttgaa attgaagata taagagatga tttaaatgga
     3661 tatgatgtgt acgattactt agataaaact gttatgccag gcattatgaa ttgtcatgtt
     3721 cacttgacaa tggagccagt tggaaatcca aagatatatt atgataatgt atcagatgta
     3781 gagcttgtag taaatacgat aaaacaactt gatgcttatt tggattcagg agtaacatat
     3841 attagaagct taggttgtcc caaatatgtg gatgtacaac ttaaaaatct tatagacaaa
     3901 gggtctatag atggtcctgg tatagttact tcaggtccag ttatatgtat gacaggaggg
     3961 catggatatt attttggtat tgaaagtgat ggagtagatg aatgtagaaa atcagccaga
     4021 acagttttaa aaaatggagt agattgtgta aagataatgg caactggagg tgtaacaact
     4081 gatggagttg agccagggtc accacaatta actttggagg aaatgaaggc tgctgtagat
     4141 gaagctataa aagcaggtaa aataactgcc acacatgcac aaggaagaac tggtataaaa
     4201 aatgcagtgc ttgcaggaat tacttctata gaacatggtg tttatttaga tgatgaaata
     4261 attgatttga tgcttgaaag aggaacttac ttagttccaa ctgtggctgc accatatttt
     4321 attctaaatg caggaaaaga aagtggaata actgaagcta ctcttagaaa gtgtgagatt
     4381 gttgccattc cacacaaaga gtcattccta aaagcatata aaaaaggaat taaaattgca
     4441 gttggttctg atgcaggaac ttcttataat gaacatggaa aaacatatta tgaaatgaaa
     4501 ctaatgtcag attatggtat ggataatatg gatataatag ttgctgctac aaaaacagca
     4561 tctgaacttt taagaattga taaaaattat ggaactctag aaaaaggaaa gaaagcagat
     4621 gttatagttg taaatggaaa tccagtagag aatatagacg tacttgcaga tgtgttagca
     4681 gtatttaaac ttggtaaaaa agttagataa atagtataaa aataaaaaag agtgtctaaa
     4741 gaaattttta aataattata gacactcttt tttattagta ataattaaat cttttatact
     4801 ttagaaaata gattctattt tatatttaga tataactctt taaaatcatt cttatgcaat
     4861 ataatctata gaatctacac atacaaagta tattcttgaa tcattagaat ttccaagtac
     4921 tataacatca tttttcttac ctagaacttc aacaccagtt agaaccaaac taccagctac
     4981 tacagtaact tgattattta tatttgacat ataaagatta tctaggatac ctttattaca
     5041 acagcaatca tcacaatcgc agttgcaatt acattctttg taatgttttt tatcaagata
     5101 gtaacctaaa gatgctatga aatcttcata atctgcaggt gtaatatcaa atgcaacagc
     5161 tttaagatta cacaatgatg ctaaatctac gtttgctaat ggagtagatg cagcaagtat
     5221 aaaatcaatt atagcagcaa gtattgcatc taatatagcc ccaaggtcta tcgtaggagg
     5281 aataactgca tcaataagtg caattaattc agctactcct ggtatagttc ctatagtatt
     5341 aattaatccc tcaagagtta aagggactgg aataggataa gatactctac cagctatatc
     5401 tattaaatca cagttacaag cagaaaatct ttcaaaagta ccatcaagtc cagataaatt
     5461 atcttttgga ggagctgaaa gatctatacc taccaaatta gcacctacta taaagaaatc
     5521 tgagataaaa gcaaattgat taaagtttac aaaaggtctt agagcatcat atcttagaag
     5581 ttctaatgct tctctcatac tttttttaca acaacattgt tttattttag gttctttata
     5641 tttatttgga taattatagt atctatcagc tacttgataa ttttcatagt atctttcatt
     5701 tgtatttgga taatcttcct catattcttg tgtaaatcta aagtcctctc tacatttatt
     5761 attttccact aaatacactc cttttatatt ttaatttaaa tgtatagata attaagctta
     5821 tctatatagt aatattatat ttataatgta aaaaaatgtt acaaaagata ttataaacga
     5881 ataaaaaata ataatttgtc agataaactt gtaaattttt atgaataatc cattctaaaa
     5941 caaaaaactg tgtataaaaa aggtttatat gttatattta tataaatata acatatgaac
     6001 gagaaaggag tggtttgata aatgattgag gtagataaat tgtgtaagtc ttttacaagg
     6061 gttgtaaaag atgataagaa taaaagttca ataagaaagc taaagaaagt aaaaactaaa
     6121 aaagaagaat tttttgctgt aaataatgtt tcatttaaag ttatggaagg tgaaattgtt
     6181 ggaatattag gtccaaatgg tgcaggaaag actacattac ttagaatgtt aggtggcata
     6241 ttaacaccaa catcaggaag tataaatatt tctggatatg attattcaat agatagaaat
     6301 tcagcaaaaa aagaaatagg ttatctctct ggaaatacaa aactatatgg aaggctttct
     6361 ccgagagaac ttctaacaac ttttgcaagt ctatatgaga tgtcaaaaga agatatagaa
     6421 gaatcaattg aaaatattgt aaaaataatg gatatgagtg aatttataga taatagaatt
     6481 gaaaatctat caactggtca gactcaaaga acatctatag ctcgttgttt aatacattct
     6541 ccaaaagtat atatttttga tgagccaaca ctaggtctgg atgttttaag tagtgcatct
     6601 attatagatt ttatgaagaa tgaaaaacta aggggaaaaa ctgttcttta ctcaactcat
     6661 tatatggaag aagcagaaac tttatgtgac aagattttta tgattcacaa tgggaaaata
     6721 attgcatcag gaacaccaca gagtttaaaa gaggaatctg gagtaaataa tttgagagat
     6781 gtattcataa aacttgccag aaaggaggac atttaaatga gaagtaagat tgtaaaatac
     6841 atatttaaaa aagaaatgtt agacatattg agagataaaa agactttatt tatgatgatt
     6901 gttgtgccac ttcttttata tcctttactt atgttattaa tgattcaagt aatgaatatg
     6961 tctgcaagta gtatgaatag taaggacatt aatatagctt ttaataataa acctaataag
     7021 gtgttagtat ccatgattga agatgataat ttagaaaaag atactaaaaa tacttcaaat
     7081 gataaaggca aaataagagt gttagatgta gataactata aaaagtcatt agaagaaaat
     7141 aaaattgatg cttatataaa gatggaagaa aaaaaatcat ctataaatta tcaaatatat
     7201 ataaattctt ctcaagaaaa ttcttctaat gcactaaaaa gaattgaaac tgttttggat
     7261 aaatataaaa gatttaccat agaaaaaact ttatctgaaa aaggattaga tgtacaagct
     7321 actttagaac caataacata tagcactgta gatgttgcaa agacggaaga agtggcagga
     7381 tactttttag gtcagatact tccatttata ttaataatag gagttttact aggttcaata
     7441 tatccagcaa tagatgttat ggctggagaa aaggaaagag gtacattaga aacattattt
     7501 acactgccaa tatcaaactt agaactggtt atgggaaaat atatggcagt ttctcttagt
     7561 gcaatagtaa cagcaatttt aaacatttta tctatgggac tgactatggc atttatttta
     7621 gttagtggag gtattagtag ccaatttggc tttggaaact ttaattatgg agacttagtc
     7681 ttaccaatta taactacttt aatttgcata tgtctttttt caatggtagt ttcagcaata
     7741 agtatgtgtg tatgttcatt agcaaaaagt tttaaagatg ctcaaaacta cataacacct
     7801 cttatgttaa ttattatgat accatcatat gtttctatga taccaaatat agtattagat
     7861 agagtgacag ctgtaatacc tgtagtaaat atatctttac ttataaaaag tgtattatct
     7921 tttaaaagcg atttaaattt aatagcctta gttttagcat caaatgtagc ctttgttata
     7981 ttggcaatca tactactgtc taagatgttc aattctgagg aaatactttt tggtaatagt
     8041 aagagttttt ctttcttaga gaaaagaagt aatataaaaa aagggtctat accaactgta
     8101 agtgatggtg ttattttata tgcagtagga ttagttctat tgatttatgt tggttcttta
     8161 gttcaaatta aatttggtat ggcaggtatt ataatgacac aattcatgat aatttcttta
     8221 ccattattat ttgcatacta tattaaagct gattttaaag aggtatttag cttgacagtt
     8281 cctaaattaa agcatgtgtt tgggagcatt gttctttgga ttggtggata catacttatt
     8341 atgattataa cacagctaat tttattctta tttcctcaaa atatggaagt tgctgaaagt
     8401 ttaaataagg ctctttttat caaagatagt gttttactga atttactagc aatagcttta
     8461 ttaccagcta tatgtgaaga aatgtttttt aggggattta tattttcttc atttagtaaa
     8521 tcaaaagata aaaataagtc tatcaaactc gctataatat gtagtggggt actatttggt
     8581 attatgcata tggattttat tagaataatt ccaacatcaa tattaggtat tatatttgca
     8641 tacagtgtgt ataaatctgg ttcaatattt gtatcaatgt tattgcattt tttaaataat
     8701 agtgtagcag ttgtgttaaa tcattataca actggaaata taaccaactt atataaattt
     8761 gttgaagttg atttttcgaa cttaaatgtg cttaaatttg ccttaattct tctaataagt
     8821 cttatattaa ttatgttagg attaagagtt ttagatagaa agtctttaag aaattaagta
     8881 taaagttatt atatagatta aaatattaat aagcactaaa gtatatggat atatcttgta
     8941 tataaaaata ctttagtgct attttatata ttttaattat atgaattatt tatcgtttca
     9001 tttatgtaat gaaaatgtta tataaatgaa acaataaata atttttattt tagaaattta
     9061 tatttaaata atcaagttga aagtgagata ctaaaaaaat taatttagga attatttgat
     9121 ttattcattt ataaaaatta attactgatt tgtcgaaacc attgtaataa gaaaaaaatg
     9181 aaaaaataaa gtgtgaatat gttgcaaata ctgtatttta ataaaaagta gtcatttctt
     9241 gtaaaatgga tagttttgaa aagaaaatat taaaattata atataaaaat aaaatttttc
     9301 agacaattta aacaaaaata atcacctttt aataaataat tatatgccaa aataatccaa
     9361 tttattttct attataccta tatgaaatat ataaatcaat agttaagaca atgaatatgc
     9421 t
//
LOCUS       DAYAOY010000135         8126 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYAOY010000135 DAYAOY010000000
VERSION     DAYAOY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.138"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            3..671
                     /locus_tag="ACGI6B_003593"
     CDS             3..671
                     /locus_tag="ACGI6B_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9753002.1"
                     /translation="MKIKDILSEILSSYPINYNIYEFSAGEELLNNYPKDLDILIMDI
                     QMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRYILKPINKEKISKSV
                     LPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIYTHDDMYITKMSISK
                     IEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKYRVKGLKLAITNILG
                     DIVC"
     gene            665..1981
                     /locus_tag="ACGI6B_003594"
     CDS             665..1981
                     /locus_tag="ACGI6B_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9753003.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            1993..2133
                     /locus_tag="ACGI6B_003595"
     CDS             1993..2133
                     /locus_tag="ACGI6B_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFG9753004.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2192..2773
                     /locus_tag="ACGI6B_003596"
     CDS             2192..2773
                     /locus_tag="ACGI6B_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFG9753005.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            complement(3098..3511)
                     /locus_tag="ACGI6B_003597"
     CDS             complement(3098..3511)
                     /locus_tag="ACGI6B_003597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753006.1"
                     /translation="MLSDLNNALKNGSLISITLSNILVDVKYKNTDDKVIFSIKLFII
                     TEINDITIDKIVSGKNHSNININIVDLIVTIDNVLIKLEYAIAYIIGMQQNIKLFLVT
                     ATEIPKKYQNYFYIIFLQYHNFFSYKKLHLTWLKY"
     gene            3447..4628
                     /locus_tag="ACGI6B_003598"
     CDS             3447..4628
                     /locus_tag="ACGI6B_003598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753007.1"
                     /translation="MLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSAYV
                     IDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYRDE
                     ERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDEIF
                     DIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKYIA
                     TKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIFEG
                     KSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASVDA
                     KKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILIID
                     K"
     gene            4643..5515
                     /locus_tag="ACGI6B_003599"
     CDS             4643..5515
                     /locus_tag="ACGI6B_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9753008.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5516..6304
                     /locus_tag="ACGI6B_003600"
     CDS             5516..6304
                     /locus_tag="ACGI6B_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753009.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(6400..7233)
                     /locus_tag="ACGI6B_003601"
     CDS             complement(6400..7233)
                     /locus_tag="ACGI6B_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HFG9753010.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(7301..7930)
                     /locus_tag="ACGI6B_003602"
     CDS             complement(7301..7930)
                     /locus_tag="ACGI6B_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HFG9753011.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         3273 a          790 c         1142 g         2921 t
ORIGIN      
        1 gaataaaaat aaaagatata ttaagtgaaa tattaagttc atatcctata aattataaca
       61 tatatgaatt ttcagcagga gaagagttac taaataacta tcctaaagat ttagacatat
      121 taattatgga catacaaatg aaaacaataa atggtatgga tactgctagg aagataagag
      181 agtttgatca taagttagaa ataatatttg ttacttcatt tgtagagttt atgcaagaag
      241 gttatgaggt aaaagcatac agatatatac taaagccaat aaataaagaa aaaatttcta
      301 aaagtgtatt accttgtata aatgagatga tgaaaaagag aaataattac ttaactataa
      361 atgtaaaaaa ttatgtagac cgaattaaaa tagattctat tacttatata gaaacagata
      421 gaccgaatat attgatttat acacatgatg atatgtatat tactaaaatg agtatatcta
      481 aaatagaaaa gatattaaat gaatatggat tttttagatg tcataatagc tatatagtta
      541 acttaaaatt agtagaatca atgagtggaa gtactgttat agtagatgga aaaagtattc
      601 caataagtaa atatagggtt aaaggcttga agcttgcgat aactaatata ttaggggaca
      661 tagtatgcta acaaatttaa gtgtaggatt ttggaatatt attaatttag tttcaggtac
      721 tatagattgg attatatttt ttttagtaat ccacttagtt ggaaagagga aaatatctaa
      781 tataagatat atatttacaa caataattat aattatattt atggcataca taaatgtagt
      841 taaagtgttt ccaaatatta agattcttct atgtatggta ataggaatat cattttatat
      901 aacatcatat gaagataaaa tctataaaag tataatagtt agtttattgt tttggctagg
      961 attaatggtt gctgaaggtt ctgcagtagg tctagtagta tttataaata aattaaataa
     1021 tattaacata acacaacatg aaaacttatt tagaatacaa gctattatta tgtcaaaggt
     1081 atttttattt ataggtttta tattgtttaa gtattttaaa ttatctttag attttaaacc
     1141 taaagatatg atactaatag gaatacctat attatctaat atagttagct tattacttat
     1201 atttgaatat aatttaagag tcaatacaat tactacagaa aacttattta tatttatatt
     1261 tgcaatttta ttgattttat catcaagtat aatactttta atagtaatag ggaaaattgt
     1321 acaagacgat aaattaaatc tagagtatga acttataaat gaaagaataa aaacaaacta
     1381 caggaattat gaaaatataa acgaagttca tgatagatta aaatatgttt atcatgactt
     1441 gaaaaaccat atgatttgta taaaaaatta tgacactaaa gaagaaatta tatcatatat
     1501 aaataactta gagtttcaga taagtgaatt tgaacatttt aaaaatactg gaaataaaac
     1561 attagattta attttagcag aaaaaataag tatttgtaaa aagtatgata ttcaaattga
     1621 agataacata aatatatcta aattaaactt tattaaaaat aatgatatat gttcaatatt
     1681 tgcaaattca ctagataatg caattgaagc atgtatggat ataaataatg agatagaaaa
     1741 aagaatagaa gttaaagcta catatataaa taaatttgca ataataaaat ttataaatac
     1801 taaggtaaat gatattaaac ttattgataa acgtattcaa actagtaagg atgatgataa
     1861 gatacatgga ataggacttg ctagtattaa atatatagta aataaatatg gtggtgaaac
     1921 aatagttaat tactcagata atgagtttat attaaaaata atgataccaa taaaaagtta
     1981 ggagagtaca aaatgaaaaa aatagcgtta aacttactta aaaacatctc agcattaagt
     2041 tttgggatag ctgtattatc agcaaacagt gcaagttctt gggtagcaca tcaggcaaaa
     2101 gagccacaag ctttacaaaa actaaaaaaa taacattaat aaaaggacct cttcttatta
     2161 aaggagaggt tttacatgtt gaggaggaaa tatgtttaaa agattatcat ataaatttgc
     2221 caatatatta gttaataatg agattgtaga aagtgaagat tttgaaatat atagatatgg
     2281 atttgaaact ttgatttatt tcatagttaa tattttggta gcattattta taggaattat
     2341 ttttgataga tttatacata cagttatatt tttaagttgt tattgcactt taagacagtt
     2401 cacaggaggt tatcatgcta ggaattataa agagtgtaca ttaacatttg cagttattta
     2461 tttgataact attttttcag ctaataatat agatataaat aaatataagt atttattagt
     2521 attattaatg attattagta tactaacaat ttacaagtta gctcctttag aacatagaaa
     2581 caaacctctt agtgaaagtg agaaaaagca ttatagaaag actgtgcaaa aaatactatt
     2641 cgtaataata tgtttaatta ttttatgtaa aatacttaat atttttcagc aatatgtgat
     2701 ttatgcaata atttctatat attggattgc tatattaata tatattggaa tgaaggttaa
     2761 tagtgatcag taagattata aaacattttc taaattaatt aacttcacta attaatatta
     2821 actttaaaaa aataatgtca aggtaagtat ttgaacttat taaatatagt tgaatctatt
     2881 gtaggttgta tcaaaagact ggctaggagg ggcataaata tgagtagtat taaggctata
     2941 tcagacttta taaccaaaat aattagaaaa aatattttat ttgcaataac aattattatt
     3001 ataaattacc atctcataca acgatggaca catatatcca atagaggaat ttcatttaat
     3061 gattacttat ccttgagtgt atatacttgt atgacaatta gtattttaac catgttaaat
     3121 gtaatttttt atatgagaaa aagttatgat actgtaaaaa tattatgtag aaataatttt
     3181 gatatttttt aggtatttct gttgctgtaa ctagaaacaa ctttatattt tgttgcatac
     3241 caattatata tgctatagca tactcaagtt taataagaac attgtcaatt gtaacgatta
     3301 aatcaactat atttatattg atatttgaat ggtttttacc acttactatt ttatctatag
     3361 tgatatcatt tatttcagtt attataaata atttaatact aaagattacc ttatcatcag
     3421 tatttttata tttaacatca actaatatgt tggatagagt tattgatatt aaagaaccat
     3481 tttttaaagc attatttaaa tcacttaaca tatttgaaga tcaatcatat gcttactatt
     3541 ctgattttgt agggaaagtt aataattcag catatgttat agataagatt atacctacac
     3601 tatttgcaat tactttaata atagtagcat atctgatatt aaatgatatt agaaagaaac
     3661 agattaaatc attaatagtt atagtagtgt acagtatttt atttattgga ttaaattata
     3721 tatcaataga tatgtcaaca tatagagatg aggagcgtag ttatggagag tatttaaaga
     3781 aatctaatat tagtgatatt aaaataaaat cgcatgatat gaagattgac tttaaaagca
     3841 agtcaaatat aaaagataga attgttttat taaacacatc aaatgaagag ttaacaaata
     3901 ttaatatgat tttagatgaa atatttgata taaaatatat aaaaataaat tcggaaaata
     3961 taaaatttaa caaaaaaaag gatttaattg aagttaattt aaaggaaagt ttagagccta
     4021 atgacgaggt gacattagat atagagtatg aaggctatat taatgtgttg acatcttgga
     4081 atcaaaataa atatatagct actaaagatg atattatgct tccaccaggt tctttagcgt
     4141 ggtacccaaa agtaaatcaa tcaagttgta tggcttataa aataaattta agttctaaaa
     4201 ataatgtcta ttcaaattta cctttactga ataagactag taatttttta aaaaaagatt
     4261 atatatttga aggaaagtca aatgatattg caatatatgc aggtgagttt aaagaagagg
     4321 taaaagatga tataaaaata atctatccat cagatagtaa tgtaaatatg gatatagagg
     4381 aaaaaattaa atttttaatt gaaacaagaa atgaaatatt aggtgatagc aaagcatctg
     4441 tagatgctaa aaaatatgta gatgaagtag ataatgttat ggaatcaata aaaagaaaaa
     4501 aaattaatat gatattagtt gctgacttac ctagcaatga atttttatat gaagactcaa
     4561 agggagagaa aattagcata actatttatc attggctttc aggaaatatc cttataatag
     4621 ataaataata aaggaggtta atatgataaa caagttagaa ataagaaatc taaattttaa
     4681 ttacaaaaat aaaaaagctt tggataatat aaatttaacc ctaatagatg gagtagttgc
     4741 attattaggt cctaatggtg ctggaaaatc aacattaatg aggttattag tgactcttta
     4801 tgaaacttca attggagaga tagaattaaa taatataaag tatagcaaaa acaatgagaa
     4861 aatcaaggca aatgtaggat atgtacctca agattttgat atgtataata atataaatgg
     4921 gcaagaatac ttagaatttg tagctaaaat gagaggtgta tctaagaatg atttaaagaa
     4981 acatatacaa aaagtagtat caaaagttaa tctggacaag tttataaata aaaaaatagg
     5041 tacatactca ggtggtgtaa agagacgact aggtatagca caggctttaa taggtgattc
     5101 taagttaata gttatggacg aacctacagt tggtcttgat ccagagcaaa ggaatgaatt
     5161 tagaagattg ttaccagaaa taagtaaaaa ttctatagta ttaatttcaa ctcacatagt
     5221 agaagacata caatttaact gtgacaaatt aattatatta aatcaaggca aaatattata
     5281 tgatggtaca attaataagt ttattgatat ggttgaggtg tattcggtag tagtttctaa
     5341 tgatgaattt gaatatttag aacgtaatat tgatattatt gattatagga gagttaaaga
     5401 tggtgtgcaa gtaaagtata taggtaaaaa tacagagaaa cttaaagata gcttaaggat
     5461 agatacaact ttacaagatg cttatataaa ctttttacat aaggagttaa aataaatgtg
     5521 tatattggag ttaataaaat ttgagcttaa aaaaatagag cttaaacatg tgttaatatt
     5581 tgtaattatt tctatcttat gcatatttat tacaggcata ttagattata atgagttaat
     5641 attttataat gatgtaccta taatggatac atattctata gcaggatggt taggaattat
     5701 aaagatatca gatgttttta taccaataat gtcagcaatc acaataattt atgtgttttc
     5761 aaaagattat tcgggtaata taaatgagat acttacttta tataataaaa aaaaatataa
     5821 tcaatttata gtatttagat ggtgtgttat attagctata tatactgtat tattattaat
     5881 ttcaattaca atagtatata catttacgtc agtgaaaggt tttaacatag acaaaataga
     5941 ttttgtttat agtgtaaagc caatagatgt gtttttaaag acatttccta cattattatg
     6001 gtatacaaca tttccactaa tgatattaac attaacaaaa aatagattta caacaatagc
     6061 aatattaatt acatatacat ttgctgatat attttttatt ttacatatgt acccatttgt
     6121 tagtatgatt aatgtaaatg gattttatat tcaaaagatg ttttatatgc ctaacaatat
     6181 agatgttaga tttaatgaat taaagaatta tttctttata aatagaggtt ctctagtagt
     6241 tatttctata ataatagttt cttatatagc aagaaaatca acggttttaa aaaagagtaa
     6301 atagtttaat atatttttta taatgttata tttatataga atagtgactt taaaaaattt
     6361 aaagccacta ttttttaata tgttttatct atataactat tatttttcta ggttttttaa
     6421 tatttcgtaa tagtgtgttg tggctatttc acttgactcg ccttttttac aaaaagataa
     6481 tttagatagt tttggaacta cacgttcaac gacaatttta ttacctatca ttttagattc
     6541 aagtatcttc aatgcttcta aaaatctatt atcctttttt gcacaattat aaaatgataa
     6601 aacgtagaca taaaggaata ggttataatt acggaaagga tattcaactt gcataaataa
     6661 ggttccaatt ccatagtgac aagggccgat tggtttttta atagtccaat ggtcaagtag
     6721 aaaatctaca gctttgtcaa gttttgattc tttatttaga taattactaa aacggaatgc
     6781 atttaatata ttaagagttg gtagtggatt tgagtattca gtttctggac ctcgaccaaa
     6841 actaaattta ttgcaacgcc acccaccatc agtatactgt gtatctaaaa aatgttgaaa
     6901 cgttttttgc attctaatgt cagatgcata tcccatatga cagagtacgt tagcagcatt
     6961 tgcagtatga caagggtaaa tacctccatt tggatatagt ttgaaacgtc catcttctcg
     7021 ccatgtgcta aatatcaagt cagcacattc tttaagtaaa gtatcatcag ggttcacacc
     7081 taattctaat agataaagta cactatcaag agtagagaaa ggagaacctt ttattaaacg
     7141 cttatcaggt gtagtccaat agtctgcacc attatcatac ctgtgtgata atattatttc
     7201 aatatctgat aaagattgat tttctacatt cattttttac tccatttcat attatattct
     7261 attaaatcta tcaaaaactt tattataata ttatttatat ttataattga tttattttat
     7321 ttaaaattgc atttatagct gtatcaggaa caactgctaa atcttctgca ttgaagtaat
     7381 aaactatatt agaatcaaat aatatatttg aatttggagg aaattcatca tctccaaacc
     7441 aaagtataaa taccatagat gtattaccca tatatgtaaa cctataagct aaatctccca
     7501 tgtttacttt ttcagctcca attttctcca taactaactt atatttgtct atttgagatg
     7561 taaaaacttc tgaaagccta tatagagtcc tgtttaaaaa atttggataa taaacattgc
     7621 ctcctggtat ttctttaaaa gttacatatt tattagttct aggaatacct ttagcattga
     7681 ttaaatatct aactatcaag attttaagct caaagttatt gcaaaggcta ccattactat
     7741 tccaaacttc tccacttgga tagtctataa tatattgtct atcaaaaaaa ttgagtgtga
     7801 atctatttaa atcattattg aaaggtatat tacttaaatt actaattgta tgagggtcta
     7861 attctttgag tttttttcga acatagtcaa atggaacatt ataattattt tttgttatct
     7921 tttcatccat aattttttcc tccaatttaa acaattaaaa gtatatttat aaaaagtttt
     7981 cttattatta attttattat atattcaaaa tattacaata ataaattgtt aaataaattt
     8041 ttatatttaa gtaaatacgt gtaaaatgaa tataaagttt tgaaatataa agtttttgta
     8101 tctaagatat tttatttgta aaatag
//
LOCUS       DAYAOY010000136         8116 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAYAOY010000136 DAYAOY010000000
VERSION     DAYAOY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.139"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..774
                     /locus_tag="ACGI6B_003603"
     CDS             <1..774
                     /locus_tag="ACGI6B_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9753012.1"
                     /translation="SNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIIIVIIL
                     VSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAIIGTSL
                     VLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFK
                     KGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDV
                     KNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            889..1674
                     /locus_tag="ACGI6B_003604"
     CDS             889..1674
                     /locus_tag="ACGI6B_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753013.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1966..3285
                     /locus_tag="ACGI6B_003605"
     CDS             1966..3285
                     /locus_tag="ACGI6B_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9753014.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3360..3788)
                     /locus_tag="ACGI6B_003606"
     CDS             complement(3360..3788)
                     /locus_tag="ACGI6B_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753015.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3879..5255)
                     /gene="hydG"
                     /locus_tag="ACGI6B_003607"
     CDS             complement(3879..5255)
                     /gene="hydG"
                     /locus_tag="ACGI6B_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HFG9753016.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5256..6341)
                     /gene="hydE"
                     /locus_tag="ACGI6B_003608"
     CDS             complement(5256..6341)
                     /gene="hydE"
                     /locus_tag="ACGI6B_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HFG9753017.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6377..7585)
                     /gene="hydF"
                     /locus_tag="ACGI6B_003609"
     CDS             complement(6377..7585)
                     /gene="hydF"
                     /locus_tag="ACGI6B_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HFG9753018.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7600..7851)
                     /locus_tag="ACGI6B_003610"
     CDS             complement(7600..7851)
                     /locus_tag="ACGI6B_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HFG9753019.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2900 a         1118 c         1178 g         2920 t
ORIGIN      
        1 agtaatcttg actttatgaa agctactgta aatattatag aaaatatagg tatatttatt
       61 cctatgggaa tttttttacc tatagtatgt aaaaatctaa ataaaaaaac tataattata
      121 gtaattatac ttgttagcct agcatttgaa ttaacacaat atatttttgc tcttggaagt
      181 agtgatatag atgatgttat tttaaattca ttaggtggca taattggaat taatatttac
      241 ataaacatga ataaattatt tcccaatgat attaacagat ttaaggctat aataggtact
      301 agtttagttt taggagttat aggattagga gttatttcta aaaattatca taacttacta
      361 acttttaaat ttagacctga tagaaaaata tcaaaaatat taattgaaga aaatagggaa
      421 ataattaaag atataaataa agatacagta gatatagttg ggacatttga gagctttaaa
      481 aaaggaataa taactattaa agcaggttct aataacaaag taaagagacc agagaattta
      541 attgattctg atggaaatat aagaatttat ttaaatgaaa atacaaaatt agtatcttat
      601 ataattacca aagaatcaaa aatggatata gttaaatatg aggaatttga tgtgaagaat
      661 ttagatttat taaggagata tgatactatt aatgtttgga ttgataaaga aaatcagcca
      721 aaggataaac atggtattat ggctagtaaa ttattaattg gattgtatga ataaaatatt
      781 aaacaagaat aaaagaataa caaatataaa aaacgaaaaa actatttcta taattatttt
      841 tacgttgtgc ataaaataag aacattagtg aaggagtaga gataaattat ggagataata
      901 aaatatatct tgtacttttt tatatattca tttttaggat ggacagtaga aagtatagga
      961 tgttctatag caagcaaaag aataatcaac agaggttttt taaatggtcc aatatgtcct
     1021 gtttatggtt ttggagcggt aattgtgata tcactattgg gaagatttaa taatgtagta
     1081 atagtatttc tattaggaat gattttaact acgatactag aatattttac aggatttatt
     1141 ttagaaacct tatttcatgc taaatggtgg gattattctg acagaaaatt caatataaag
     1201 ggtagagtat gtttaaaaaa tgcaatatac tttggtgtaa tgtctgtttt aataataaga
     1261 tttatacatc catttataaa atattttgta agtataattc catatagaat attaatttca
     1321 atggctataa ttacaacact ttggactata cttgatttga ttgttactat tataacactt
     1381 aaaaaattag atataaagtt aaacttacta gacgatataa ttacagacct taatgatata
     1441 aatgtgaaat tggataaatt tgatagagga gaaatacaag ctttatttaa aacaataaat
     1501 aacgacgaat tagaagttag agaaaaaata aacaaaataa atagtaagtt ggaccgtata
     1561 gagtctaata ttattttaca aaaaagagtt ataaaagctt ttccacatat aaaacataaa
     1621 aaacaacaag agcaactaga acattttaaa aaaattatta atgagaaaag gtaattatac
     1681 aatttaatga agacgaatag aataatggta tttataagat gattttcaga ggactctata
     1741 aaaatagaat agagtccttt attttttgaa aaataatgtt tatttgataa aaataaaata
     1801 tattgtttct gaataacaaa tattttgaaa attataaaaa catatattga caatttgtgt
     1861 caaaaatact aacattaagt atataaagaa ttttatatga gaatatcttt ataaatcgca
     1921 ataattttaa aacataaatt tatttaaaga ggggatgatt tgccaatgag cgagataact
     1981 agtcaaatga taagtacaga agaaaaaaaa tatgttgcta aaactcaaaa gataccttat
     2041 tatccagtag catttaaatc aggtgatggt gcaatgctat atgattatga aggtaatgaa
     2101 tatgtagatt ttttagcaag tgcaggctct gcaaatgtag gtcatggtaa taaggaaatc
     2161 tcacaagcag ttaaagaaca aatggatgat ataacacaat atacacttgc atatttccat
     2221 agtgatcctc ctgtaaaatt agctgagaag cttgtggaaa tagcccctgg agataatgat
     2281 aaaaaagtac tatacagtgc aactggttct gcttgtatag atgctgctat aaaattagca
     2341 agaggatata ctggaagaac aaagataata tctatgtgtg aatcttatca tggaagtact
     2401 tatggagcta tatctatatc tgctttaagt acaaatatga gaagaaaaat gggtccattg
     2461 ttaccagaag tttatcactt ccattatcct gataaaaata gaacagctaa agaatgttta
     2521 gatgagatag aatatgcttt tgcacactat ctaccagcag aagaggtagc tgcaatattt
     2581 atagagccaa ttgcaggaga tgcaggaata atagtgcctc cagtagagtg ggtacaaggt
     2641 ttaagtaaaa tatgtaaaga aaatggaata ttacttgtaa gtgatgaaat acaacaaggt
     2701 atgggaagaa caggtaagtg gtttggtata gaaaattttg gtgttgaagc agaccttata
     2761 gttcttggaa aatctgttgg aggtggatta cctctaggag cagttgttgg aagaacagaa
     2821 ataatgcaaa gtcttgatgc accagcacat ttatttactt tagctggaaa cacaacagta
     2881 tgtgtggctg ctttaaaatc tatagagata attgaaaaag aaaacttact tcaaaagagt
     2941 atagaaatgg gagattatat taaagcagga tttgaaaaac taaaagaaaa atacgatata
     3001 attggagaaa taagaggcat aggattatct ataggagttg atatagttaa aggaaaaggt
     3061 tctaatgaaa aacatccaga tgctacagct aagatatgtt atagatgtat acaaactgga
     3121 ttaataatga tattcttagg acaatctact ttaagagtgc aaccacctct agtaataact
     3181 aaggagcaag tagataaagc aatgaacatc atagattctg caatagatga ttatttaaat
     3241 ggtagaattg gtgatgaagt atatgaagta actcaaggtt ggtaaaataa atattaaatg
     3301 aaaataggag ttatctcaat tttagaaaat tatttctgaa atgagataac tcttttaagt
     3361 tacccacaaa taagaaaatt gtttgattca ttaaagacta tatttcggat gtcgtttttg
     3421 tgagtatcag agacagtatt acgagaataa ttacctttat tcgtactatc tgttaattta
     3481 gttataatta agatagctaa aagaataaga attactgtgt ataaagcaat aacatatggt
     3541 tttcttttat atttgtattt gttattgaaa aaagaatggc cataattatt tttatttact
     3601 ttagatgtat ttgtattctt tttcttgtta cctttctttt tatcatcctt ttttctattt
     3661 ttagggtaag gaatgacttt gtgtgtatta tcaagtttga tatttttatt tttttgaata
     3721 ttgttttcca ggctagcctt tttttctcca aattgaatag ttttattggt attgttgtta
     3781 tctttcaatg ttaatctccc tcccttaaac tttcccttta gataatatta ctactaacct
     3841 atggaatata caataaataa tatataagta ataaaatttt ataggtataa atctctttct
     3901 ccacgctcta aggcagtcat attgttagat actatgtttc ttatatcttc tctttctatc
     3961 ttacctattt cttctgcgat aagtttttta cccataattt taagttcttc atctccataa
     4021 tcgattaaaa attcatttaa tgtagtaaga gcatttggtg tacatacatt atggatttgt
     4081 cgacttttag ctaagctcat gaatctatcg ccagttctac ctttacgata acaagctgta
     4141 caataactag gtatatatcc atcagtaatc agttctttaa gcacctcaac agggcttcta
     4201 tggtctccaa cttcaaattg ttctgtattg tcaccatctt catatgcttt atatcctcca
     4261 acacctgtta gagaacctgc actaatttgt gatacaccat attttagtaa ctcatttctc
     4321 atttcagcag tttctctagt ggacattatg attccagtaa aaggaacagc caatctagtt
     4381 atagctacta tttttttgaa catatcgtca gaaactaagt gaggaaaatc ttctaagctc
     4441 ataccttcag cttttttcaa tctagggaaa gatatagtat ggaaacctac accaaatttt
     4501 tcctctaagt gttcgttgtg catcataagt cctagaactt caaatttagg gtcagataaa
     4561 ccaaataaaa ctcctgcacc aacatcatca acaccagctt ccatagctct atcaaaagct
     4621 gttaaatggt aataatagtc attttttata gattgaccat gcatttttat aaatgttggt
     4681 ttgtgataag tttcttggaa taatatataa gttcctattc ctttttcttt tagtaattta
     4741 tactcatcta cacttgtagc tgctatgtta acgtttaccc ttcttatatt tccgttttca
     4801 ttatatgttg aataaattgc atctaagcaa tctaagatgt aatttatatc acaattttta
     4861 gggtcttcac cagcttctag agcaagtctt ttatgaccca ttttttctaa tattttaacc
     4921 tcttccttta tctcttccat ggtaagtttt tttcttttaa atttgttgca tttactaaaa
     4981 ccgcaataaa cacattcatt tacacagtag tttgatacat aaagaggggc aaataaaacc
     5041 actctattac catatatttc attttttatt tgcccagcta tactaaataa tctatctagt
     5101 tgttttttat cttctacttc aagaagtgta gctatatctt tataactaag tttttccctt
     5161 ctatcagcct tatcaagtac cttttcaata tcatcagaag ttgaattttt tgcatcttct
     5221 aataaagaat taataagttc atggtttatg aacatttaca aacgcctcca ttcaatatta
     5281 tcaccgcgag aatagtcaac ttcaagtcct aatgacttga tgttttcttc taaactttgg
     5341 tgatattcac aagcttcttg accagtaaag gctttgttat cgtaaagaga atattgtttt
     5401 ctaaattctt gaggagaaag gtttggcata attacattgc aaccagctaa aagacctgca
     5461 tttctaccac tagggtttat agatgccaag gctgttgtag cgggtaataa aacttttgga
     5521 agtagaagtc ttgtaattga aagcataagt aatgtcttct ctaaatctcc atgtttataa
     5581 tccttaaaaa ttgtatcatg gtgtggaata aaaggtccta ttccaaccat atgaggattt
     5641 aactctttta ggtataataa atcacgaaca aggtcttcgt tgttctgaaa tggtgattca
     5701 accataaatc cagcacctac ctgataacca atttctttaa ggttttttag gcattctttt
     5761 ctagttctaa gctcaatgtt tggcggatgt aattttttat aatgactgtc tgtagcagtt
     5821 tcatgtctta aaagatatct atctgcacca cataaaaagt attttttata cgaatcataa
     5881 ctcttttctc ctagtgatag agttatggca caatcctcat atttattttt tatacttgaa
     5941 acaatttcac atattttatc atcagtaaaa tatgcatctt caccaccttg caaaacgaat
     6001 gttctgtatc ctattttata acctatatca caacattcaa gtatttcatc aagtgaaagc
     6061 ctgtatctta tgatattttt attagaagat tgtattccac aatagtagca attattttta
     6121 caatagtttg taagctctat aagtcctctc aagtagactc tatttccata atgtttattt
     6181 ctcatgtcgc tagcttgttt ataaagatag tttcttaaat tttgttcgaa gttatattcc
     6241 tcattgtcga tattttctat taaatatagt agttcatcaa aacttaatga attaatgctg
     6301 taaagtttat ctataaagta tttaatctta tttttagtca tattcaaata tgtaattatt
     6361 aagtatagtt aaatttttaa taattgaaaa gttcaagtga tctttccaaa atacctttta
     6421 catatgctat taagacacca tagtttacaa taggtacatt gaaagatttt gccttttcta
     6481 ttctagataa cattccagct ctattcatca tacaagctcc acagtgaact acaagggcat
     6541 atttatttat atcttcagtg aaggatactc cagaactaaa ttcaaaagta atgtttttgc
     6601 cagttttctt tctaatcata ttaggaattt ttactgttcc aatatcatca gtttgtctat
     6661 gatgggtaca tccttcggcc atcaagattt tatcaccatc ttttaaattt tctaaagcat
     6721 aagctccatt aatcaattct ttaaggtctc ccttttgtct agcaaacaat atagaaaaag
     6781 atgtaagagg aatatctttt tgtgtatctt tatctacttg tggaaatact tgagagtcag
     6841 taataacaag ttttggtttt ttgcctaaat tagatagagt ttcttttaaa ctatcttctt
     6901 ttgtaactat agaaattgca ccattatcca aaatatctct tataacttgc tgttgaggaa
     6961 gtataagtct accttttgga gctgctttat ctattggaac aactaaaaca actaaatcat
     7021 ttggttcaat taaatctgat accaatttga actcagtgct atccttaggc aaaactttta
     7081 ttatttcatt ttttaaattt tcaattccaa ttttatctgt tgatgagaca gaaacaactg
     7141 gacatttaac tttattttta gttaagttta aaatttctga ttgattctta atagtatcaa
     7201 ttttgttaag aattagaata tgagggatat tcttatcatt aaatttttca attagtgaca
     7261 aatcttcttg tgatatacca atttggcaat ccactactag taaagctata tctgtctttt
     7321 ctaaaacatc tagtgattta cctattctaa gttcaccaag ttcaccaaca tcatcaagac
     7381 cagcagtatc tataagaaca caaggtccta taggaaggat ttccattggt ctaaatactg
     7441 ggtctgtagt agttccagca atatctgaga ctattgcagc actttgattt gttatagcat
     7501 ttattatgct agatttacct gcatttcttt taccaaagag acctatatgt actcttaccg
     7561 attgtggagt agaatttaga ctcatttgta ctcaccacct taaacttctt tttttgatat
     7621 actagttttt acagatacat gaggtatgtt gcccaattta ccagtcaaac tattaatagt
     7681 gtccatttcg ccaacaactg ctatacatac gattgagaca ccttcttctt cgcaaggaat
     7741 gcccattctt cctttaataa tacctttaaa acctgataca acctcattga atttaaattg
     7801 acattctttt ggttcttcca aaatagcact tataactgca acctttttca taaatatcct
     7861 cctaactcag aaaatatgtc ttaaacaaat ccaaaatcaa aaaacttttc tttgtaaaga
     7921 aaagtttcta caaaagaaaa gttattaatt acacaagtat aatgtgctat attaaactat
     7981 ttccttacag attagataac tcttttctgg tagggttagc ttgtaaaaat ttaatttaac
     8041 aacaagtaac tgaagaaaat ttttatacaa tttatataaa ttataataaa aattaactaa
     8101 agaaaattga agttac
//
LOCUS       DAYAOY010000137         7953 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYAOY010000137 DAYAOY010000000
VERSION     DAYAOY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.141"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..301
                     /locus_tag="ACGI6B_003611"
     CDS             <1..301
                     /locus_tag="ACGI6B_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9753020.1"
                     /translation="YIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFEKLEV
                     YFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQLT"
     regulatory      394..486
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            586..1380
                     /gene="thiD"
                     /locus_tag="ACGI6B_003612"
     CDS             586..1380
                     /gene="thiD"
                     /locus_tag="ACGI6B_003612"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFG9753021.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1404..2192
                     /gene="thiM"
                     /locus_tag="ACGI6B_003613"
     CDS             1404..2192
                     /gene="thiM"
                     /locus_tag="ACGI6B_003613"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFG9753022.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            2215..2847
                     /gene="thiE"
                     /locus_tag="ACGI6B_003614"
     CDS             2215..2847
                     /gene="thiE"
                     /locus_tag="ACGI6B_003614"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFG9753023.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            2986..3288
                     /locus_tag="ACGI6B_003615"
     CDS             2986..3288
                     /locus_tag="ACGI6B_003615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9753024.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3354..4103)
                     /locus_tag="ACGI6B_003616"
     CDS             complement(3354..4103)
                     /locus_tag="ACGI6B_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753025.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4097..5059)
                     /locus_tag="ACGI6B_003617"
     CDS             complement(4097..5059)
                     /locus_tag="ACGI6B_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9753026.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5063..5521)
                     /locus_tag="ACGI6B_003618"
     CDS             complement(5063..5521)
                     /locus_tag="ACGI6B_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9753027.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5877..6233
                     /locus_tag="ACGI6B_003619"
     CDS             5877..6233
                     /locus_tag="ACGI6B_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9753028.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6305..7021
                     /locus_tag="ACGI6B_003620"
     CDS             6305..7021
                     /locus_tag="ACGI6B_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753029.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7014..7838
                     /locus_tag="ACGI6B_003621"
     CDS             7014..7838
                     /locus_tag="ACGI6B_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753030.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
BASE COUNT         3095 a          915 c         1287 g         2656 t
ORIGIN      
        1 atatattata agatgtaaag atgattctct atacactgga tataccagta atatagttag
       61 aagaatgaat gaacataaat taggaattaa ttcaaaatac acaagagcaa agggatttga
      121 aaaactagaa gtttattttg tgaccaacac aaaaagcaat gctatgaagt tagaatacta
      181 tataaaaaaa ttaactagaa ataaaaaatt gtctataata aaaaacccaa gtatacttat
      241 aaatttaatt gataataaag aagattatat tattggtaag gaaatagaac aattaacata
      301 aatatttata gtaacattat aatgggtgca tatggtgtaa aaacttgaca ataaatacta
      361 ttgtaatata atgaagttat aaatatagta aattaaaata gctaggggag ccaagagctg
      421 agaggtatac cgacccttac acctgatctg gataatacca gcgtaggaaa gcttagtaaa
      481 aattcaagta tgtattattt gtgatatatg ctatttctaa gctataccta ttgtaggtat
      541 agctttttta attaagttat ttaaattaaa ctttaggagg caacaatgag taattataaa
      601 ataccaacac ttacaatagc cggctctgat tcatctggag gagctgggat tcaggctgat
      661 ttgaaaacgt ttagtgcaat tggtacttat ggtatgagtg taataacagc tataacagca
      721 cagaatacta aaggagtttt tgctgtagag gatttaaata agaaaataat aaaaaaacaa
      781 atagaggcag tctttgaaga tatacctcca agagctgtaa aaataggaat ggtttcaagt
      841 cctgagatta tacttgaaat agttgagaat ctaaagaaat acaatccaaa atatttggtt
      901 gtggacccag taatgatatc taaaagtgga tattatctat taaagccaga ggcaaaagaa
      961 aacttaatta agtatttgat accacttgca tacataatta caccaaatat accagaggca
     1021 gaagaaataa caggaataaa aatacataat gtagatgata tgaagagagt aggagaagaa
     1081 atactacaac ttggacctaa atttgtactt atgaaaggag gtcatttaga tggagaagca
     1141 gtggatattt tagttggtaa aaatatattt aaagtttata aaagtgaaag aattgataaa
     1201 aaaaatacac atggaacagg gtgcacatta tcttctgcaa taacatctta tctagcatta
     1261 ggatatgaaa taacagaagc agtaaatcta agtaaaatct atataacaga agcaataaaa
     1321 agaagttttg atataggtca tggagtagga ccagtacatc atttttataa atttgagtaa
     1381 taaaaaaata ttggaggtag aatatgtata atttaattaa agatgtaaaa aaattaaatc
     1441 cattggtaat tcattatact aataatgtaa ctataaatga ttgtgctaat gtaactttag
     1501 ctgtaggagc aagtcctctt atgagttttt catatgagga agtagaagaa atggtaagtg
     1561 tagcaaactc tgtagtaata aatataggaa ctatgaactc aaatatgttg gatttattcc
     1621 tattagctgg aaaagcagca aataagtaca ataaacctgt tgttttggac ccagttggag
     1681 tatttgcaag caaagcgaga gcagaactta caagtagact tttaaatgaa gtcaaattta
     1741 gtgttgttaa agggaatgta tcagaaataa agtttatagg tggctttaat gtaaggggta
     1801 aaggtgtaga ttcatttgat gaagaagaag attctactga gataataaga aagattgctg
     1861 aaaagcttga atgtgtagtt gtagcaactg gaaaaataga tataataact aatggtaaag
     1921 gtacatataa aataaacaat ggaactgata aattaaaagg aattacagga actggatgta
     1981 tgactgctag cttgattgca agttttatgg ctgttactga gaatatatta gaagcagcaa
     2041 caatgggtgt tcttacaatg tctttaagtg gggaattagc taatttaaat aatccaccaa
     2101 taggtacatt taaagaaaat cttatgaatg ctatttatca aatggatata gatgctttaa
     2161 gtaaaaattc taatattgaa tttttaaatt aataaagagt aagggagact taaaatgatt
     2221 gataaagaat ctttaaaaaa atgtttaaag ttgtatttag taactgactc tgaaatgctt
     2281 aaaggtagag acttttataa atgtcttgaa gatgcaatat cttctggaat aacaactgta
     2341 caacttagag aaaaaaatgc ttctggaaga gagtttttaa gaaaagctat gaaacttaga
     2401 gaaataacta aaagatatgg tgttaagttt ataataaatg atagagtgga tatagcactt
     2461 atttgtgatg cagatggagt acatgtagga cagagtgaca ttgatgttag agaggttaga
     2521 aagcttatag gaaacaataa aattctagga gtatcagcaa gaactttaga agaagctatt
     2581 tgtgcaaaaa atgatggcgc agactattta ggtgtagggt ctatattcac aacatctact
     2641 aaattagatg caaaatcagc ttcgtttgaa actgttaaag aaattaaaga aaaagtagac
     2701 atgccatttg tactaattgg aggtataaat ttagacaata tagataaact taaatgttta
     2761 gaaagtgacg ggtatgctat aatatcagct atattaaaag ctgaagatat ctctaaagaa
     2821 gtggaaaagt ggacattaaa aatataaaat tggctctggt agtgaaaaaa atggaaaaat
     2881 ttgatataat ctaagagtaa aatttaagca attataaatg ataaactttt ctacatatac
     2941 tttttaatca agatatatgt agatttggtt aaaaaggagc tataaatgat taggatattg
     3001 ttagtgtgtg ttggaggcat gtcttctacc ctattggtaa ataaaatgga gaaggatgca
     3061 aaaaaaagaa atatagactg taaaatatgg gcagttggag aaggagatat aaaatcggag
     3121 cttgataact ttgatatttt acttcttgga ccacaactta gatttatgct tgatgacgta
     3181 aagtctatag ttggagatag agctccagta tctataatag atatggtaaa ctatggcact
     3241 tgtaatggtc atgctgtgtt aaattcggtt ttagaaatat taaaataaat aataaaagtg
     3301 gattctatat aatagagtcc atttttatta tttgttatta ataggcttaa aacttagctt
     3361 accatttctt tttttaatct ccacatagca agtgaaaaac ttgaacagca caatattaaa
     3421 aatagtagga taacaggcat tatatctcca ataagaatag tctcaccaaa tattaagcta
     3481 cgtaaagcgt taataacatg ggtaaatgga ttgagcatca ctgctacttt taatcctcca
     3541 gataaacttt ctatagggaa aagagctgaa cttaaaaaga aaataggaag tacaattgca
     3601 gtcataattg tttcataaac tatttcattt ggcaataata aacttataga ataagctaga
     3661 cttgataaaa agaaggctgt cataaatatc agtactatca taagtaggat tccaccaatg
     3721 ccagattcta tccttactga gaagaatata cttactatac acaaaatagt tacttctatg
     3781 aaagaaacta agatagcttc taataattga ccaaggacga ttgaatacct gctgataggt
     3841 gcaattaaga ctctatagaa acttccactg ttcttcatta taaagtttat aataccacca
     3901 ctactacaag aagaaaacac aaccagtaca atgatacctg gtagtataaa agcagtataa
     3961 ttattgatat ttatattatt catagtttgg tttgcaatag aactataaag cacaagccaa
     4021 agaaaaggtt gtaatatagt aactacaaat gagattggat ttttaaaacg ccacttcata
     4081 ctacgccata agatatttaa cattcccatt cctcctttaa gcttttttta gaacttgtaa
     4141 gtgctaaaaa tacatcttcg agacttggct ctacaatttc tattgcatca aattctatat
     4201 catgttctaa aagccattta tttattaaag taaaagcagt tctactatcg tttacactta
     4261 taaaaattga attttctcgt acgctcataa actttactaa accagtactt ttaattgaat
     4321 ctttatattt ttttatatct tcagtattat gaaaagttat acgtagtata ttttgtctaa
     4381 tgtaactacg taaacttgat ggtgttcctt gtgctaaatc ctttccattc ttcataatac
     4441 aaatattatc actcaattgt tcagcttctt caagataatg agtagttaga aatatagttg
     4501 tgccatactc atctctaatt tttaataaca tatcccacat agattttctt gaatcgacat
     4561 ccataccaac agttggttca tctaaaaata ggattttagg agatgatacc atattcatag
     4621 ctatgtccaa tctacgtttt acaccccctg aatatgatga agtaggatat tttaagtaac
     4681 tagataagtc aaacttatca attaaagagt ctattctttg ttttgcaatc tgtggttcta
     4741 ctttatacat tttactttga aataccatat tttccataag ggataaatgt tcatcaatag
     4801 agatttgctg tgctacacaa gcaatttgag tacgtatcca agaaggatta tctactaaat
     4861 cttttccaaa cattgttaca tttccagatg taggtttgta aaaggtagtc aaaatattta
     4921 taagagaaga tttaccagca ccattaggac caagtaaaga aaaaatttct ccagcattta
     4981 cattaaagct tagaccattt aaagctttca caccattttt atattctttt actaaattgt
     5041 ctatttcaat cgctaacata tattattcct cctctttaag tggaaatatt atctctgtaa
     5101 tatagttatc tgggtttcct tttatcaaca tacctggtcc tttaataaat acttctctcc
     5161 aaggagtttg tatttttata ttattttcat gaatatattt aaatatcatt tcatacgttt
     5221 ttggcaaatt actataagag cctgtgtgag taaaaaataa agcttttgtt ttaggaaatt
     5281 cttttgtagt tatacccata cgatttggag tttcagcagt tggaacacat aattccattt
     5341 ctgcaacacc agttttttgg tcaacatcat agtagcaaaa gaatggtgca ccgttagact
     5401 ttccttttat agctttaaat acatttggaa aatactttgt tgcctcagtc ataggtccat
     5461 tataacgcat ggtagccact gtgactgatt cgatattttt gatttcaatt ttgcaattca
     5521 tttgtttacc tcctaatgtg tgttcatatt cttactagat ttttaagcat taatacatat
     5581 aagattttga ttagttgaaa acatataatc ttatctcgtt tgcttgtttt caagtctaac
     5641 ataacataaa atatatatcc tctcatttga tgctccattt tgtaaagtta ttatttaagc
     5701 tttactttag attttttagc aaattaaaat aaagcaattt ataaaataag tatttacaaa
     5761 ttaagtgtat taatcgtata atacacttaa taggatacta gataaagtct gaaataaagg
     5821 cgtatttttt taaagtaagt gtactatcgt attaatacac tagaatagga ggaatcatga
     5881 attttgaatt agataataca actcccattt atttgcaaat agtaaagtat ataaagagac
     5941 aaatagtaac aggagagttg aaaccaggag agacaatacc atcaagaaga gaaatggcac
     6001 ttaatctaaa agtaaactta aatacagttc aaagagcata taaggagatg ggtgatatga
     6061 atataataaa tacttttaaa aattatcaaa gcagtgtaac agttgatgaa aatatattaa
     6121 aaaatttaaa attagagctt ataaatgaat cactatctgt ttttatagaa gatatgaaag
     6181 caataaatgt tagcaaagaa gaagttttaa aaataataga agataagtat tagtttttta
     6241 acaaatagaa aacttagttt agaaagttta tttataagca tattggatgt ggaggatagt
     6301 aaatatgata gaaataaaga atgtcagcaa aacatataaa aggatgcaag ggcttaaaat
     6361 aagaaagata gaagctttaa aaaatgtaag ttttaatata gaaaagggaa aaataactgc
     6421 attacttggt ataaatggtg ttggtaaatc aactatgtta aaagcaatag caggacttat
     6481 aaacattgat agtggagaaa ttcgtataga tggagaaaaa ataaatgaaa aggtatacaa
     6541 taaattggct tttgttccag atgtacaatc tcactttagc aatacaacaa taaaagaaac
     6601 atttgaattt atggaaattt tttattcaaa gtggaataaa gaaaaatcaa aagaaatgat
     6661 ggacatattt aaattggatg aagatgaaat aattgataac ttgtcaaaag gaaacattgc
     6721 aagagtaaaa ttaatacttg gattttgcca agacccagag tacatacttc ttgatgaacc
     6781 atttacagga attgatttgt ttaagaggga agagtttata ggagtaatag cgcaatatat
     6841 ggaggaaaat caagccatta ttataactac tcatgagata gtagagatag aaagtttagt
     6901 tgatgaggtt gtaattcttg atgaagggca gattataact tcatttaatg cagaagaatt
     6961 aagagaaaga gaaggtaaat ctatattaga taagatgagg gaggtatata agaatgaata
     7021 aacttctata tctaataaat gtagatttga gaagaagcaa taagttttat atagcttata
     7081 tatcattttt ttcattaatt acacttagtt taaatttatt cgaaattaat aaatttaaaa
     7141 attctaaatt tattatgaac ttagcaatag aagattttgg aggtatattt tatggactag
     7201 ggatacttag taatcttggt tttatccaaa gtatattgtt ctttgggata ttgggacttt
     7261 ttatatacta tatttttatg tggaaaagag agttcttttc aaataatcaa agtatttata
     7321 cacttatgat gttacctcaa aataagttta agatttgtat atctaaatca atagccttga
     7381 taacaatgat atatggtttt ttgataagtc agacagcaac attatttata tgtaaatata
     7441 tatttaattt catttttaga aatatgccta taatcaatat gaactttgcc aaagatttat
     7501 attatttaaa cttgaagagt ataccaattg attttatctc ttttatggct atttatgtat
     7561 ttttactact tatagctatt tctattgtaa attgctgtat attatttatc ttttcctata
     7621 taaataaatt ttatgtaatt ttaatcactt tgctgattat agcaggctta tatttttcaa
     7681 caatatatat aaatataatc actttattta gaaaatatta taatcttata ggatttcaat
     7741 ttaacaattt agtagcaggg ttattaagtt ttttaattat aatgtttatt ctaaatggaa
     7801 tatcttattt gctaattaaa aagaaagtat ctctttaagg aggggtggta tgaaattaaa
     7861 atctaaaata tctatattta ttatattttt gactttagca atatatttta gtataggttt
     7921 attaaataat gaagataatt taaagataaa aac
//
LOCUS       DAYAOY010000138         7884 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.142, whole genome shotgun sequence.
ACCESSION   DAYAOY010000138 DAYAOY010000000
VERSION     DAYAOY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.142"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(308..1126)
                     /locus_tag="ACGI6B_003622"
     CDS             complement(308..1126)
                     /locus_tag="ACGI6B_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9753031.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1294..1875
                     /locus_tag="ACGI6B_003623"
     CDS             1294..1875
                     /locus_tag="ACGI6B_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9753032.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            1872..2060
                     /locus_tag="ACGI6B_003624"
     CDS             1872..2060
                     /locus_tag="ACGI6B_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753033.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2230..3972)
                     /locus_tag="ACGI6B_003625"
     CDS             complement(2230..3972)
                     /locus_tag="ACGI6B_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HFG9753034.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            4401..5777
                     /locus_tag="ACGI6B_003626"
     CDS             4401..5777
                     /locus_tag="ACGI6B_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HFG9753035.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            6088..6759
                     /locus_tag="ACGI6B_003627"
     CDS             6088..6759
                     /locus_tag="ACGI6B_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFG9753036.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            6948..7745
                     /locus_tag="ACGI6B_003628"
     CDS             6948..7745
                     /locus_tag="ACGI6B_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFG9753037.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         2915 a          961 c         1216 g         2792 t
ORIGIN      
        1 tatctgacaa attaagataa tatcccaatc aaattctagt catttaaaca taacatcaca
       61 agtgatttat ctgacaaatt aagataatat cctgattaaa ttctagtcat tcaaacataa
      121 catcacaagt gatttatctg acaaattaag ataatatcct aattaaattg tagatattta
      181 tacataacac taaaagtaat ttgtttaaca aattaagata atatcctaat taaattgtag
      241 gtatttatac ataacactaa aagtaatttg tctaacaaat taaaatatta tccaattaat
      301 ataaaaatta cttcttttta actgggaagc atacctctgt tacccaatta tcaggattac
      361 tatcgttgcc aggacttaca tgataaatat taaacatagg accatccaca tcatagttat
      421 tatctgatat ccaattacca atagcctcac aagcgtctgc aacttgattg aaatttccat
      481 taacaattgc agttgcagca gtcacacttg gaacagtctt aaatttaaca tgttcagtat
      541 ctttatactt tccagataca gcaacttgta cttcaacatc tacataatct tctttatatc
      601 cagtatcata aaaaactgct ttagaatact ttgggggttc aatttgaaca ttctggtctt
      661 ttgtctctaa aaatgcttga tgccataaca ttccttcgtc ttgataagtt ggtataaccc
      721 ttctaagtgt catcatatat tttggtgcaa aattctttat agttacatca taatttttca
      781 taatatcatt tcctccaatt cttttaatag tggtttctat ttttaatagt tttttctgtg
      841 tatcttctaa ttgttctttt acttgagaat ggtggatatt tagatatttt atgagacttt
      901 ctttatcgtt atattctgtc aaaatttctt ttatagaata caagccaaag cccatatctt
      961 ttagtgcatg gattctgttt gtagtagaaa gttggtctgc actatagtat ctatatccat
     1021 tagttttatt cgtatgagaa ggagttaaaa gtcctatttc atcataatga cgtaacatac
     1081 gaatactaat tttacttagc tttgaaaaat cgcctatctt aaacatgttt ttgtctcctt
     1141 tataaaggta cttttagctt aatattaata taaaccatat cataatgtta gagtcaacta
     1201 aaattataat taaattatat tgacatatga tgaaaaaaat attatactga acatgttcag
     1261 aactgaaaat gttaattgtt ttggaggtct attatgaaaa gtaatgataa agatgctaga
     1321 gaacgtataa ttgaagttac attgaatctt ttaaatgaag tagatgatat tgaggagatt
     1381 acagttagaa aaattgctga gcgtgcaaat gttggagttg gtttaataaa ttatcatttt
     1441 aaaacaaagg ataatttgtt gagtactgcc ataggagatg tgatgtcaaa tataatagca
     1501 gagctatatg acgatagtgt atatactcta agacctattg aagatttaaa aaatttatta
     1561 aaaaaattgt gtgatactgg attacattat gaaaaagtgc ttccttttgt attgaatcaa
     1621 tgtataacca atggagatat gcaagcagaa cttgatattg tccctatgct tcgtaaaatt
     1681 tttggaaata aaaaagatga aatgagtcta agaattattg ccctgcaaat tattttacca
     1741 atacagattt ctgctttatc tacagaatca tttcagttat acagtggtat aaatataaag
     1801 aataaatatg agcgagataa attcattgat attttaatag aaaatattat tggagaggat
     1861 gttgatgtaa gatgaaaatt ataactattg gttcaagtct tatcactgtt ttactttttc
     1921 tttcaactat gatttgtggg ttctggatta agaataataa ggtaacagat gcaagttcta
     1981 ttaagttcca tatgaatagt gctattttta cagggatatt tttattgatt tccacaatcc
     2041 ttttaattat atatatataa aaataataat attattaata aataaagtat cagaaattat
     2101 aaagacaaga gtcttaaaaa tttctgatac tttaccatat aattttatta aacattaatt
     2161 gtgtgtgaaa aaatatcact tatttcaatc taatactaaa tcttaagagt gagtataaag
     2221 tattagaaat taagaagcat tttttagttc aagcggatta tctataccaa aataatcata
     2281 taaagtagag cttatttcct ctaaatttcc atcttggaaa ggtaattgta aatttacctt
     2341 ttctaccaca tctttaaatc cagcatcagt attttgattt aaaaaatcca tatatacatc
     2401 aacagctttt ctgtaatctt cttgtgataa ttgccatatc tgaagtgata catcagatgc
     2461 aagaccataa ctcaagtaat aaaaaggaac ttggaagtta tgagatacaa gtatccaaaa
     2521 aggagcattt ttttctctaa gtatattatt ggaaactcca tattcttcct ctaaatcaaa
     2581 aaacaattta ttgatatcat ttagactcat atatggattc ttgtatataa tttcttgaaa
     2641 ttcatcataa agacaagcat caataatagt atttaaaaca atcgataagt gttctttttt
     2701 tatagcttca gattgtttac caaaaaaatc atcaaaatat ttataaaata gaatttctaa
     2761 agatgttgaa tgaacttcac atacatctat acttggttgc atcttattgt ttaagttaat
     2821 actgttgtag taattataaa aatgaccaaa ctcatgagca aaactagtaa cacctaaaaa
     2881 agaattatcc catgtattaa acaagaaagg tgctttatat ttatttatat aagtagtata
     2941 tcctccaggt gatttgtttt ttccagaaga ataatcgtac aagtcatatt ttttcatata
     3001 gtcgaagctc tcttttagtt ttggagatat atcttcaagt actttatcaa acttcctaaa
     3061 tgagcgattt ttatatactt taatataaat agaagaatca gatggtttag atgaaatctc
     3121 tctatataat ggaactatgt aatcttttac atcttgtcta aattttttag cttcttcttg
     3181 agaatagtct ttgtttaggt tcatataggc ataatctata taattcttaa atccttgctt
     3241 acttgctatt tcagttcttg tctgtataag ttctaagaat atgttaccaa atataggatt
     3301 gtattttttt aagtaatctg aataaatttt cacatattct tcagggctaa gatttggtct
     3361 tgacaaggct tcttcaaagt ctacctctat accatctatt gatactgtag atttagctaa
     3421 taagtcttca tattttacaa ctaggtcttg ctctttttta agcaaacctt caacttcttt
     3481 agaattaagt tttccaatgt tctttagata attaaatgta tcctttccaa gttctttttc
     3541 taattcttta gagaatttag aagttacaaa tctatcttga aagtctttgt atgacatgtc
     3601 aacatctact gatttactca taagatactt atattcctta gagtaaaatt cgtctgatga
     3661 atctatgtta tttctaattt ttgctatagt aagtgtactg taaaaatctt gataaatttt
     3721 atcaacttca ttaaataaat ttaattgttc cttagctgtt ttagctgtta aaagtttgtc
     3781 gttgcacttg ttaatgtttt cgcaaattga ctgtatatta ggacgcttat attccatttt
     3841 ttcaaaagga atagttcctc taacagaatc tttttttgta tcattagtat aatttcttat
     3901 gtatgatgca aatataataa aaactgcaat taaaattaga aaaacaaata ttttcttctt
     3961 ggatttaacc ataaaaatcc tccaatcatg gatatagtta ttaattattt tactatatta
     4021 aatataaaaa atgcatgtta agaaaattta acataaagat atagataaga ttataaataa
     4081 tataaaaaat ttaacttcaa aaaagtatat gaaatttata ctggtaaaat gataaataaa
     4141 ttttaagtac aaaatagtaa aagctatgat agttttagac ctattatatg taaaaaatat
     4201 agatgttttt ggtgcaattt aaattaaaag atatatataa aaatttaaaa tattccaaac
     4261 agaaattata aaaattacta tcattgtctt tatacaatat ttatactata atagaataat
     4321 aatttagagg aaagctctaa aaataaacaa attaagggtc ttaacagtag tgattttatg
     4381 ttaaggaggt ataaagaaag ttgaaaacaa tagttagaag acttattctc tacgcagatg
     4441 gaatgcgtcc tactcaaata atggtatctg ggtttgctgc gattattgta atcggagcat
     4501 tgttgttaac actcccgata gcgtcgcaga gtggagagag tataggctta ttaaatgctc
     4561 tatttacggc tacttcagcg gtttgtgtta ctggtctagt tatggtagat acagcaactt
     4621 attggagttt atttggacag atagttataa taacattgat acaaattggt ggtcttggat
     4681 ttatgaccgt agccacaatg ttttcactta tggcacgaaa aaaaatacaa ctaagagaaa
     4741 gattattaat acaagagtca ttaaatcaag ctgatttatc gggacttgta agacttacaa
     4801 gatttgtatt aataataacc ataacaatag agggaatagg tgcactggtt ttatcaactg
     4861 tctttattcc acaatttggt ttgtctaaag ggatatggta tagtgtattt cacgcaatat
     4921 cagcgttttg taatgccgga tttgacttga tgggcagtgt aagtggccca tttacatcgt
     4981 taaattctta tgtaaacaac tttacagtat caatgactgt ttgcgcactt atagtgcttg
     5041 gagggctagg attccctgtt gtacttgata ttgtaagaaa aagaagattt tcaaaattaa
     5101 atgtgcattc taaggttgta ttgttttcaa ctgctacact tatttttgtg ggtgcattat
     5161 ttatttttct tatagaattt aatcaaaaag ctactatggc ggacttgcct ttaaagggaa
     5221 aagtactatc agcaatattt caatcagtaa cagcaagaac tgctggtttt aatacgcttg
     5281 atttagctac tttacgagaa agtagtgtat ttgtaatgat aattttgatg tttattggag
     5341 catcacctgc atcaactggt gggggtataa aaacaacaac actagctgtt ttaatcatta
     5401 cagttagaag ttttctatca ggaaaatcag atatagaagc ttttgaaaga agattagctc
     5461 catcaactat aaaaaaatct cttggtatat ttgtaatcag tatatcagca gttatttttg
     5521 gaacattgat tatttcaata actcagccta attttacatt agttcaatca gcttttgagg
     5581 ttacatcagc acttgctaca gttggttcaa gtttagctgg aactccaaac ttaaatgctt
     5641 taggtaagat tataataata atctttatgt ttatgggtag agttggttct cttactttat
     5701 ttatggctat actttctggt ggtagaagaa agagtcaacc aatcagatat gcagaaggta
     5761 aaattatggt tggttaagtt accaaggagt agtataaatt tataatgggt ttagagtagc
     5821 actgtaaatc tgttgataaa ataaatgagc ctagatgtaa ttatatgaag aaataaaatc
     5881 aactaatgaa caaaattaat taatgaaata aaaaaacagg gaaataaaat acaaataaaa
     5941 aggtaaatca aaattaaatt aatctgtgtg tataaaatta tatattgtgt ataagttttg
     6001 tatgtgtaaa gtatataagt tttgtatgtg taaacaatta atttaagaga tattatataa
     6061 attgtgtatt agggaggaag tttcgagatg aagcaatata tagtcattgg gtgtgggaga
     6121 tttggaagtt cagttgcgtc tactatgcat cttttaggac atcaagtaat ggcaatagac
     6181 aaaaatgaag attcagttca aagtatatct gacaaggtaa cccattcact tatagtggat
     6241 gttactgatg agcaagcgtt aaggtcatta ggtttaggta actttgatgt agcagtagtt
     6301 gcaataggtt ctgatataag ggcatctata atggcgactc ttatagccaa agaaatgggt
     6361 gtagagttga taatatgtaa ggcaaaggat gaattacaag ctaaagttct ttataaaatt
     6421 ggtgcagata gagttgtatt tccagaaaga gatatgggag taagagttgc acacaattta
     6481 gtttcggata atatattaga ccatattgaa cttgacccag agtattcaat tgttgaaatc
     6541 gtaactccaa atagttgggt tggcaagaca cttatagagc ttgaattaag agctagatat
     6601 gagataactg tacttgctat aaaaacaggt aaaaatataa atgttacacc ttctccagat
     6661 gaggaactta cagccggaag tatcctagtt ataatcggtc aaaatactag tataacagcg
     6721 ataacatctg gaaataaggg gataattaga agaagataat ttactattta atatatattt
     6781 aattgcaatg aaagcagata gtatcatata attatgaata gttatatgat actatttttt
     6841 taatcgaagg tagtatttat ttgtaagatt agataaagag aagttaaatt aaaagtaagg
     6901 aggctgtgct aatataaaaa tttataagtt attagcattt agataaaatg attacaaata
     6961 taaacagtaa ggataatgaa aagttaaagt atacaagagc actattaaaa tcaaagaata
     7021 ggaataaaga gtcaaagttc ataatagaag gatacagaat agtaatgctt gcacttgaat
     7081 gtatggcaaa ccttgattat gtatttatca atgaagaatt tgaaaataag aaagaacatg
     7141 taaaactatt agaagatttg gataaaaaaa acacaaagat atacaagact actaataaaa
     7201 actttaaaga attagtggat acagaaaata ctcaaggaat aataggtgta gtttcattta
     7261 agaaaaaaaa attaagtgaa agtataaata aaaaagataa atttgtattg attttagaca
     7321 gaatacaaga cccaggaaat atggggacta taataaggac tgctgattct gctggagtag
     7381 atgccataat agcactaaag ggatgtgtcg atatatacaa cccaaaagta attaggtcta
     7441 ctatgggttc tatttttgat atgaatataa ttgatgcttc acaagatgaa actgtggaca
     7501 tgcttaaatc attggatttt aatatagttt caagttactt aaatacagaa aatttttatg
     7561 acaaaataga ttatggctca aaagtagcat tggtgatagg aaacgaagca aatggaataa
     7621 atgaagaact tgtatcaaag tctgatattt tggttaagat acctatatat ggtaaagccg
     7681 agtcgttaaa tgctgcgata agctctgcta tactgatgta tgaaataaaa aaatacttaa
     7741 tttaatgtat tgtaataaat ataatgtcat gttataatct tgaattaaat agtagagtat
     7801 caaaaatagt caatatatat taaaaaaata attaaattaa ttatatatta gatgtatatt
     7861 atataaaaaa taattgagtt aatt
//
LOCUS       DAYAOY010000139         7866 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAYAOY010000139 DAYAOY010000000
VERSION     DAYAOY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.143"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..893)
                     /locus_tag="ACGI6B_003629"
     CDS             complement(<1..893)
                     /locus_tag="ACGI6B_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFG9753038.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFL"
     gene            1153..2142
                     /gene="add"
                     /locus_tag="ACGI6B_003630"
     CDS             1153..2142
                     /gene="add"
                     /locus_tag="ACGI6B_003630"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFG9753039.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            2214..2954
                     /locus_tag="ACGI6B_003631"
     CDS             2214..2954
                     /locus_tag="ACGI6B_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFG9753040.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            3154..4305
                     /locus_tag="ACGI6B_003632"
     CDS             3154..4305
                     /locus_tag="ACGI6B_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFG9753041.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            4330..5085
                     /locus_tag="ACGI6B_003633"
     CDS             4330..5085
                     /locus_tag="ACGI6B_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HFG9753042.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            5277..7664
                     /locus_tag="ACGI6B_003634"
     CDS             5277..7664
                     /locus_tag="ACGI6B_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HFG9753043.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         3168 a          848 c         1376 g         2474 t
ORIGIN      
        1 tttaaaaaat ctataagttt aaaatttaat tctagtatat ttatcttatc atttaaattt
       61 ctaaaaataa cagccgaaaa catacaagtt acaacagagg gtaatagtat attaaacgtt
      121 atatagaagc atctacttat aatttctcct gtatttatac aaataaatat gagaagaacc
      181 tgttccaaaa atacagatgg tgttattgtt tcggagaagt tattattttt tatcctattc
      241 ttatttctaa aaaatttact tccaaatctc tttagatttc tattatatct aggaatcgac
      301 aagtctatat ttgatgatgg ttttaattta tatttattta ttagaaattt agctacagga
      361 catcccaata taccacccat aataagtcct attgttgcag cagataatcc aaccccaact
      421 gctccttcta taccaagatt ttctattgta agtccaaatg cagcagaata accatgacca
      481 ccttccatag atacatttcc acacataagc cctaaaagag gttcaagttt tataatcttt
      541 gatattgtaa tagctaatat attttgacaa aatgccaata ctccacacaa aatccaatat
      601 ctaaataata cactaccacc tttctttata agatttatgc ttatttcaag tcctatagtt
      661 gtaaaaaaga aggttatgaa atttggcatt aaatttccac ttatagatat atctgctata
      721 ttaaaatatt taaaaaataa atttagaaaa caaaataata gacctcctat tactggtgca
      781 ggtatacaga aattatttaa aaagttaact ttattcttca ataaattacc aattaaaaag
      841 aagaatatag atagtgtcaa ggtttgcact atatcaaaac ttaaggtaat caaaataagt
      901 cctcctttgt aattcaaagt tttaattata taacttaaat tcttatgtat tgaaatttta
      961 ttatcaaatg tatattaata aatgtatatt aaaaagaatc agtagatata attagatagt
     1021 cataaattaa attaaaatat tttaatgaga tatgactttt ttagaattaa aaaccgtagt
     1081 atattgatat aatagaaata agacttaaaa atctgttgat agtattattt ataagtacag
     1141 taggaggata aaatgtttga aaatttacct aagatagatt tacattgcca cttagatgga
     1201 agtgttagag tagaaaccat gcttgatata gctattaaag agaaaataga tttaccttca
     1261 aataatatgg atgaaataaa aaaattagca aaggtttctt ttaattgtac ttcactagat
     1321 gaatatttag aaaaatttga tttaccctta aaagtaatgc aatctaagga aaatttaaaa
     1381 agaattactt ttgaactttt ggaagatgca agtagagaaa atgtaaaata tattgaaatt
     1441 agatttgcac cattacttca cacccaaaaa ggtatgagtg ttaaaaatat aattgagggt
     1501 ataattgaag gaattagaga agctgaaagt atttatgata taaaaggcaa tttaatatta
     1561 ggttgtatga gaacaatgac ctcaaaagag gcattattag taattgaaga agggaaatca
     1621 tttgtaaata aaggtgttgt agcagtagac ttgtgtggtc cagagaaaga gggattttgt
     1681 aaggaatata aagatgtttt taaattagca agagaatatg gatataaagt tacaatacat
     1741 gcaggagaag ctgcaagtgg agaaaatgta ttggatgcta taaatatttt aaaggcggat
     1801 aggattggtc atggggtaaa aattaaagac cataaaaagg cgtataattt agttaaagat
     1861 aaaaaaatat tgcttgaatt atgtccaact agtaatgtac aaactaagac agtagattct
     1921 tatgaagcac atccatttta tactttttat aaagataatt tacatgtaag tataaatact
     1981 gataatagaa ctgtatcaga tataaattta agttcagaac taaatgttat atttgatact
     2041 tttaaactag gattagaaga ctataaaata atttatagaa atgcagtaga agctagtttt
     2101 gcagataaag aaactaaaga gtatttgaat agtttaattt aatagataaa aatatttaaa
     2161 ctttaaatta agctattaaa ttgataaaaa tacattgtat taggagaaaa aatgtgaaag
     2221 attatgaaga tgttgtttat attaaactag tcaaaaaaga acttgaagaa tggaagaaaa
     2281 atatattaaa gaaaccttct atatttgaca aagcatcaaa gggtatacag aataaattta
     2341 atggagtatt gccagataaa tatcatgaaa taattacttc ttctataaaa gtaatgacaa
     2401 aaaccgtact gtttggatat aaatatgcta caaagccatc acttagaaat atgtcattag
     2461 aagaaagaga aaaattagtt ttagaaaaag ctaaaactta caagactaca gcaatgattg
     2521 aaggggcagg aacaggggca ggaggaatcc tcttaggttt atctgatttt cctttgcttt
     2581 taggtattaa agtgaaattt ttatatgatg tagcttcaat atatggatat gatgttaaag
     2641 attatagaga aaggatttat atattaaata tattacagtt ggctttttct agtcaacagc
     2701 atataaacaa aacttttaaa ttgatgaata actgggatga atatgtaaat agtctaccag
     2761 aagacatcaa tgattttgat tggagaacat ttcaacaaga gtatagggat tatttagatt
     2821 tagctaagtt tttacaacta atgcctggaa taggagcatt tgtaggattt tatgtgaatg
     2881 gaaaattaat tgataaatta tgtgaaactg ctgtgtctgc ttatagattg aggattcgag
     2941 agcttaagtt ataagagaaa ttataacata aaaaattagt tatacaatat atctatatgt
     3001 atctaatttt gcattgataa aaacaattat ggataaaagg taaagcatga tagaatctaa
     3061 tgtagaggtt tgtattttaa tggaaaaatt tagtttttat tgcttaagat acaaattgaa
     3121 ttacgaagag aagtaattgg gaggaatatt ttcatgacaa atttgccaaa acaatttgat
     3181 agcttcaatg aagctagaca aaaaggattt ataaatgcaa aggaacttaa ggaaagtggg
     3241 aaaaagatgg ttggagtttt ctgtacattt actccagttg aaattccaat ggcagctgga
     3301 gcaactgtag taggtgtatg cggcgttagt gaagagccaa tacctgatgc agaaagggtg
     3361 cttccaagaa atctttgtcc tttaattaaa tcaagctatg gacatgcaat aacagatacc
     3421 tgtccttatt tctatttttc tgatttgttg ataggagaaa ccacttgtga tggtaagaaa
     3481 aaaatgtatg aagaacttgc aaaagttaag ccaacatatg taatgcattt gccaaatact
     3541 tcaaaaggag aatttgccta taaactttgg aaagatgaaa tgataagatt aaaagaagag
     3601 gttgagaaat ctttaggtgt aactataact gaagaagata taagaactgc tataaaagat
     3661 aaaaatgaag aaagagaact tttaaaagag ttttatgcac ttggtaaatt acaaccatct
     3721 gcacttacag gccttgagtt acataatgtt ttatatcaag caggttttaa atttgaccgt
     3781 gcagaactta aacattcttt aagaaaagtt atagatgata tgaaagaaag atatgaaaaa
     3841 ggtgaatgtc ctgttcaaaa agataagcca agaatattga taacaggttc tcctattggt
     3901 ggaataagcg aaaaaatagt taaaacttta gaagatgcag gggcatcagt cgttgcatat
     3961 gaactttgtg gagctattag atcaaacgat ttattggttg atgaagaaat agaagatgta
     4021 tatgatgctt taacacaaaa atatataaat ataggttgtt cttgtatgat gaataatgac
     4081 aatcgtattg aattattaga tagaataata gatgaatata atgtagatgc tgtaatagat
     4141 gtagttttac aagcatgtca tacatttaat atagaaagtt atagaataag agaatttgtt
     4201 acaaaagaaa aaaataagcc atttatgtct cttgaaactg attattcaaa atcagatact
     4261 gaacaattaa gaactagatt tgaagcgttt gtagagatgt tgtaggaata aataaaagta
     4321 ggaggaacta tgtttagtat aggagttgac tcagggtcag tggcaacaaa gggtgtgcta
     4381 tttgatggtg aaaaaattat taagaaaata attataccaa ctggatggag ccctaaaagt
     4441 acatctaaac aggtctatga gttgctaagt agtgaaattg acaaaaaaga tataaaaaag
     4501 gtggtaggta caggttatgg aaggggagta atggattttg ctgataagaa agtaacagaa
     4561 ataacttgtc atactagagg tatttacttt ttaaacaaga acataaggac tattttagat
     4621 gtaggtggtc aagatagtaa agttataaat ttagatagag atggaaatgt atttaacttt
     4681 ataatgaatg ataagtgtgc tgcaggaact ggtcgatttt tagagattac ttctaacctt
     4741 ttaggaagcg atattgaaag tatagataca ttagctaaag gatatgaacc agttaatata
     4801 tctagtatgt gtacagtttt tgctgagtct gaaattgtaa gtcttctagc tcaaaacatt
     4861 tctactggag aggtagcagc agggatactt aaatctattg caaataaatc cacctcaatg
     4921 ttagcaagag gtgaggtaat agatgaagtt gcatttactg gagggcttgc aaaaagtaaa
     4981 gaactagtaa aaatgataga agaaatactg ggtaaaaaaa tttttattgc agaagatact
     5041 caaataatag gagctttagg agctgctgta ataggattta gataaaaaca tataaaagga
     5101 aaatgagaag ctgaatttat tataaataaa ttcagttttt tattttattt tatttattta
     5161 taaaggaaaa ctttggtata attgatttaa atgattgtat atttgtaaat acttgatatt
     5221 tagtcatata taacattata attttaaaaa ttagctcaaa ataaggggga gaaagcgtga
     5281 aaaaatttac ttcaaaaaaa gtaacaaggt ttgttgcgct gtttttaata tctattttag
     5341 taatgtcaac aattcctgta tcagctgaca atagcaatac attgaatcag ataaaaaaag
     5401 cagaatacag tgagacgtat aaacaatatc taaatgatgc taaaaatgga cgtacagaaa
     5461 aatataatgg gattatacca aatccatatc agttggaagg tactcaaatc caaagtaaag
     5521 gaagaacagc tccaacatct tatgacccta gaaaattagg tcttatgaca tcaataaaaa
     5581 atcaagagga tttaggaatc tgttgggatt ttgctgcaat ggcaacctta gaaagtttct
     5641 taaagttaaa taattatgga gactatgatt tatcagaaga acacttaaga tggtgggctt
     5701 cagatggtga atatggatgg tctgtaaatg atatgaatgg tgctttaaat tatgaagcta
     5761 tgggatattt aacatcttgg tcaggtccta aactagaaaa agatattcca tacaatggta
     5821 gagtatctaa agcacaagga gcaaaaaaac caactaatat gaatacagca cctactgttt
     5881 ttaatgtgac agatgctgtg tgtgtaagta atgacataaa ttctacaaaa aatgctatat
     5941 tacagtatgg agcagtaaca tctggttatt atgaagatat taaatatcaa agtgatgacc
     6001 aaaattcata ttattgtcca acaaaaagtt ttaatacaaa tcatgctatt tcaattgtag
     6061 gttgggatga taactattct aaagataatt ttaatgcaaa tattagacca tcaaaaaatg
     6121 gtgcttggtt aataaaaaat agctggggag attataattc tgagggtgga tatttctgga
     6181 tatcttatga agacaagaca cttatgtctg atatagataa tttttcaatc aaaggtggta
     6241 aaaaaccaaa tgatgatgag aagatgtatc aacatgacta tgcaagtatt gtacctttaa
     6301 tttcaaaaaa gataacagct gcaaatgtat ttgatttcaa tagaggcgat gaaactctaa
     6361 aatctgtaat gttcttaact gaaagtatag gagctaagta tgaagtatat tatgctccag
     6421 tagtaaatgg tatacctcaa gaaaacaata tgaaaaaatt aaaagaaggt actgcccaat
     6481 attcaggata cataacagtt ccaattgatt cttttgatat accagaagga aagggtgcaa
     6541 ttgtagtcag tatagagggg aaaaatggag agtcaactat aggttctgag tcaaatgtac
     6601 caggatatga tacatttaaa gcaaaagcta atttaggtga aagttatatc atagataata
     6661 ctggaaaatt ctttgatata aatagagata gtaacttcta tccgtgtaat tttactataa
     6721 aagctgttac agaaaagtct tctggtgaga gtatcccaaa tgagtcttta attggtagtg
     6781 atagatatga aactgctatc aaagttagtc aaaatggttt caattcttca gaaaatgtag
     6841 ttttagtaaa tggcagttca atagtagatg cattggcagc aactcctttt acatctgcta
     6901 taaattcacc aatattattg acacaaaaag aagctttaaa ttcaaaaact aaagctgaga
     6961 tacagagatt aggtgctaag aaagtatacc taataggtgg agaaaattct ataagtaaag
     7021 aaattgaaca acaattaaaa agcttaaata tatcgataga gagaatatca ggaagtgata
     7081 gatacaagac aagtttatta ttagctcaaa agttaaatgg tataaaaaat gtatctcaaa
     7141 tagcagttgt aaatggagtg aaaggattag ctgatgcaat tagtgtaggc gctgcagcag
     7201 cagaaaataa cataccaatc atacttgcaa atgaaaagag cgaattgcaa ggagctgatg
     7261 aatttctaaa ttcattaaat atagagaaat cttatattat tggaggaaca gcatccctat
     7321 caaacaatct agaaagtaag ttaaaaaatc caactagatt atctggtagt agtagagacg
     7381 aaactaattc aaagataata gataattttt ataaaaaaga tactttaaaa aatgcatttg
     7441 tagttaaaaa tggaatcaag aatcaaaatg atttaataga tggattggca gttggtcctt
     7501 taggagcaaa aactgggtca ccagtaattt tagttggtga taaattagct gatagccaaa
     7561 aagaagttct taaaaataag actttagaaa aagtaactca agttggtgga ggagcaaata
     7621 aaaatgcatt taaagagtta gtaaaattaa aaagtgctaa atagagtact taagtattaa
     7681 catatattag tgtgtattag taacttatct taataaaagc tgaaaataat tacttcaaat
     7741 tattgataaa taagataatc aatgattgta aagttttgtt ttcagcttta aatttatgta
     7801 gtataagttt aaaaacataa aaaagggtta ctttatatag aaatatagaa aatcttacat
     7861 tttata
//
LOCUS       DAYAOY010000140         7831 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYAOY010000140 DAYAOY010000000
VERSION     DAYAOY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.144"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(202..345)
                     /locus_tag="ACGI6B_003635"
     CDS             complement(202..345)
                     /locus_tag="ACGI6B_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753044.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     gene            604..792
                     /locus_tag="ACGI6B_003636"
     CDS             604..792
                     /locus_tag="ACGI6B_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753045.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            1495..2016
                     /locus_tag="ACGI6B_003637"
     CDS             1495..2016
                     /locus_tag="ACGI6B_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9753046.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
     gene            2010..3578
                     /locus_tag="ACGI6B_003638"
     CDS             2010..3578
                     /locus_tag="ACGI6B_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753047.1"
                     /translation="MVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENINTDIYE
                     KEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLKAQLWRV
                     LGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEKYLMKLQ
                     NIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDIDAGFRI
                     SKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYHNKGKNN
                     DIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILIDRNLSDK
                     IEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRSDNRNNN
                     ASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAIYQVKKS
                     DGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKETLKLAF
                     KKLNENQLQESTNM"
     gene            complement(3642..4160)
                     /locus_tag="ACGI6B_003639"
     CDS             complement(3642..4160)
                     /locus_tag="ACGI6B_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HFG9753048.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(4326..4673)
                     /locus_tag="ACGI6B_003640"
     CDS             complement(4326..4673)
                     /locus_tag="ACGI6B_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753049.1"
                     /translation="MDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRAANNLH
                     ELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDIIREYE
                     TYRGRYLTFNWKR"
     gene            complement(4769..5581)
                     /locus_tag="ACGI6B_003641"
     CDS             complement(4769..5581)
                     /locus_tag="ACGI6B_003641"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9753050.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKILANVLSWGYSPSKVLVCD
                     IKDYVPGQTENLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFINR"
     gene            complement(5581..6012)
                     /locus_tag="ACGI6B_003642"
     CDS             complement(5581..6012)
                     /locus_tag="ACGI6B_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9753051.1"
                     /translation="MNLTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWIDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKKLKEKIGNLLDTMTDELNIKGGNK"
     gene            complement(6080..6298)
                     /locus_tag="ACGI6B_003643"
     CDS             complement(6080..6298)
                     /locus_tag="ACGI6B_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753052.1"
                     /translation="MKKIIVICILVLMLIIPTMGEIFIMVENIKLEGLEQNPTSCYIS
                     NPGIMGEAKCDAAYFIIAADFVNNLKVK"
     gene            complement(6396..6578)
                     /locus_tag="ACGI6B_003644"
     CDS             complement(6396..6578)
                     /locus_tag="ACGI6B_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753053.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIRESGVVEGAEPTK"
     gene            complement(6578..6871)
                     /locus_tag="ACGI6B_003645"
     CDS             complement(6578..6871)
                     /locus_tag="ACGI6B_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753054.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELDYIDIPYGS
                     IDYIKNRIIGINIETKQPILEEIPVYVSEEKKRIQELENQVLLNENEKVGGLL"
     gene            complement(6887..>7831)
                     /locus_tag="ACGI6B_003646"
     CDS             complement(6887..>7831)
                     /locus_tag="ACGI6B_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753055.1"
                     /translation="LITLLQALPFLTVSSYNSTQATFKADHTDPTLLTKIEVFIDDVL
                     KETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPENVNLN
                     DISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELSEKIKQSFDSGDASLQDL
                     MNQLTQANNTISQLNSKYKVASGTVTSFADSAKIAYPYLTDRTFKPGTWVKISNLDFK
                     PNIFFADFDYYDTEYKNNYKLFLFACRGVATQRGVDFSSVTAFIRKNSDENFHANGWL
                     YNNSEGDVYFNNTGVQIPAYNFDSTQRHIYKWYAIKFI"
BASE COUNT         2850 a         1110 c          909 g         2962 t
ORIGIN      
        1 agatagtgtt ctttttgttt ttatgaaagt tttttagggg gataataata tatatactta
       61 gagataaaat aaaatatatt tctagcattc acagttttat tttttcttaa aatgcgatat
      121 aataaagatg taaattcgta tcaaaaacaa aaaaagaaac tacaatctat tagcctagag
      181 tgaagtttca ttaactaaat attaatgtcg ttttttatta aacttgattt ttacatcaag
      241 ctcaaagtca ctcctgccag agtggctttt tacttttctg gaaataatat atgtaataaa
      301 gctagctgtt aaactagcta acactcctag taaaaattcg ctcatttctc cacctccttc
      361 cttttaaggg atggaatgta gaaaatgaag ctccactctt agattgtagt tccacaagat
      421 tattcttgca ttttaattat aacataattt tacaattatc aaatatccat tctccatatt
      481 tttttattta tacaatatat tctatattgc aataaaaaac attgttttaa atatatatac
      541 ataggtatat actaaatatg ttaaacttaa ttatggaaaa tataaaatga aaggaataaa
      601 aatatgacta agactatatt atgtgattac tgtaataaag gaataaataa agatgataat
      661 aagtatatta cttttcataa gaaaagtcat atgaaaacta acatttgtat taattgtgca
      721 ttaaatttga tagataaaga taaattaaat gaaaatatta taaataatca acatgattat
      781 tcaaagaaat gaaaaagcac tctccataat gaagaatgct ctatataata atgtttgact
      841 tagtaaagat gtgttggggt tacatattta cttttttatt atatcatata actttgtgta
      901 tgaaaaagaa tttaaatcaa ttttaaggtg tgttgagtaa tgttcttgat agtttatatg
      961 ttgatgaatt tcaaaaaaaa taagcactct tataaaaaga gtacttttgg tatatattca
     1021 agcatttatc taatacaatt atagcatgta ttatgtttta gtatgataat tttcgttcgt
     1081 tttattatta caacttctac tatagttttc atactttaac atcaactaaa tgaatttaat
     1141 taagattact agttaatcgt tttttggttc tttcatattc tgaaatctaa aattttatta
     1201 tttgtttatt gttattattt aacacatgtt ggtatttcaa cgatttatct tattgttaat
     1261 attcttattg cttccaaagt atatctaatt atttttaaga taatttacta atttttattt
     1321 tttcaaacat acattcgaca aaaaacagtt tttatatggt ataattatat tgtataatac
     1381 aaaaggttaa tgaaaataat taatattaaa tgtaccaaaa aaaatagttt ttgatataat
     1441 gaatattata ataaataatt atttaaataa aatattatat aaggatgtga ttttatggat
     1501 ttcaaaatca gagagctaat taatgatata acccaagata ttatccaaac atacaaaatc
     1561 caaattccaa tagtaaatat aaatcaagtt gttgatgctt taggaggcaa ggtaatagaa
     1621 gatagttctt taagtggata ctctgatgga tttattagaa aagttgatga ttcatttgaa
     1681 atagtggtat ctccttatca accagatacc agaaagaatt ttaccattgc tcatgaactt
     1741 ggacatttat ttttacacat gggttatggc attgatgatg aactatggaa tagtcaagat
     1801 ggaaatcagt attttagaag tggcaatacc aataaggagt atcaatccaa tgaatttgca
     1861 gcagccttgt tgatgcctaa acatgaatat aaaagaatta tggatgaaaa cacagtaggt
     1921 aataaggtcg acacttcaaa aattgcagaa tactttaatg tttcctctta tgcagcatct
     1981 aatagaggga aatggttagg atatttacaa tggtagatga taaagaatat aaaactcaaa
     2041 atgtaaataa tgttcataat tcagctaaaa acgaaaacgc ttttaatctt aaaaagtata
     2101 aggaaaaact tcaagaaaat attaatactg atatttatga aaaagaaaaa gaacctaatc
     2161 atccagaagt gattttgttc ttttcttttg atatagctaa ttcatcatta tataaaaata
     2221 taaattatag cggatgggct aaggtattat cacatataat tcgtaagtta caatatagag
     2281 tttatgaaaa ccttaaagca caactttgga gagttcttgg tgatgaggta attttcatta
     2341 tcgtactaaa aaattatgat gaaatttata aatacataga tataatcttt gatattttaa
     2401 caagcactgc taaagatata aaaagtggta atatattctc tacactagaa ggattttctg
     2461 aatctgaaaa atacttaatg aaacttcaaa atattatttc attaaaagga gcggcctgga
     2521 ttgctatagt atcaagaaat cctaatttta atgctttaga aaataatgaa caatatgaaa
     2581 atatttctgc tatgtacgat ttatccaata attataaaat atttgagttc ttaggaaatg
     2641 atattgatgc tggtttcaga atatcaaagc aaacatgccc agaaagactt gttcttagtt
     2701 ttgaactagc ttatatatta tcaagaaaaa ctgatatttt atctaaatta catataatta
     2761 catataaaaa attaaagggt atctggaaag ataaactata ccctatcatt tggtatcata
     2821 ataaaggaaa aaataatgat atagaatttg atgatagttt ttcttttgat gaaatagaag
     2881 aaaatgaatt ggttcgagaa tattttttta ataaaaaagg agaaagtaaa ttactaattg
     2941 attcttttat gtttaattct gtagacaagg ctttggataa aatacttata gacagaaacc
     3001 ttagtgataa gattgaaaaa ataggtgacg taatttctaa aacaaacccc agttatgata
     3061 aaaatacaat agataaagac tatataaaag tagatttaat ggaattacac tgtgttgcgg
     3121 tttgttataa taaatcaact tcaaaaatat taattgcgaa aagaagtgat aatagaaata
     3181 ataatgcgag taaatgggaa tttggttgtg ctaaggcaag tctagaaact tcaattataa
     3241 atactattaa agatgaatat gaaaaagatt ttaatataaa cattgaacct attactgatt
     3301 gcacaagaaa agatgattgc caacctatac ctctagcaat ttatcaagtt aaaaaaagtg
     3361 atggtttaca caaaggcatt ataactctcg cagaaataat aaatgattat gatatttcta
     3421 aatttgaacc cacatcaaaa cataatgaac ttgcatggat aggagaggat gaacttgaag
     3481 attttaatga aaacacagta cctgatttta aagaaacttt aaaactagca tttaaaaaat
     3541 taaatgaaaa tcaattacaa gaatctacaa atatgtaaat agtattcttc tatgatttga
     3601 ttctatcttc atagaggaat actaaccaaa taaaacattt tctaaatagc attaaaaact
     3661 actacaagta tatatataaa caatataata ccacctaaaa taaaagcttc tactgacttt
     3721 tttacattat acattttctt atcacagatt aaagataatt cttttatttg aacaagtgca
     3781 tcattaatca agttttcttc gtctttaaat gcattttcaa atgcttcttt gtactgttca
     3841 ttatttaaag atgctatctc tttataataa aaaatagatt tatattcatt ttctatttta
     3901 gaattccttg gctttaaaac cataatcgaa aaataaattg aaataacaaa tatacataat
     3961 ataacaatgt aaaatagcat ctctaaacat ctgatattac ttatacttaa gtgattcatt
     4021 attttttcct tccctgaaaa taaaaaacct ataattgcgc tatttaataa tagtaaaaaa
     4081 ctagctttat tatctgattt ttcaatatag taatcaattc tatttactat aaacttcgct
     4141 atgtctattt ttttatccat gattttacta cctcatttca ttaaatttta tattgattca
     4201 aaattttatt ttctacttat tatcattgaa taaattatta tttggaaata atcttattga
     4261 aataataaaa atatgtgaaa gaaagtaata actaactata attactactt cctaacttaa
     4321 acattctatc tcttccaatt aaaagtaaga tatcgtcctc tataagtttc atattctctt
     4381 ataatatcca atatttcctc cctagaagtt gcaattaaac ttctatcaaa tagttcaatc
     4441 attctattgc tatcattatc agaaacatta caagactcta atatctcatt attaaatatt
     4501 gcaatagcat acccagtacc tatttttatc tcatttaatt catgtaaatt atttgctgct
     4561 ctaaagatat cagcttcagc caatccacct aaatcttcgc ttctattaat caagttatta
     4621 agtttaatac taaaagctgc tcttacaaat ctctcataaa ccaatgcatc catacataaa
     4681 tcctccatta tcacaataat atatttacat tatattacaa taatagagtt atcacaacaa
     4741 acaaccactt ctagttgata acttttatct atctattaat aaaatccaat gctttataaa
     4801 gtgtatcaaa tctatcatta ccttttatca taataaattt ttctttagta atagaactta
     4861 tcttttcaca tgcgccacct cctacaacat ataaattttc cgtctgacct ggtacgtaat
     4921 cttttatatc acaaactaaa actttgcttg gactatagcc ccaactaagc acattcgcaa
     4981 gtatcttatc aacttctcca tcataaacaa ttgtatgttt gtacatctgt ttaactccct
     5041 cattatttat atttttattt aatacacctt ctacaattaa cttagcaata ccttcatgac
     5101 ctagtttctt agctttatca taatcttctt tattatcaca gaagaaactt tcaattaata
     5161 ctgctgtagg ctttgaacta tttaagatat ataatctttt atctaattta gcacctctat
     5221 ttttaaatac tgtacctagt ttatcacata ttctagttgc atactctaag cctttattac
     5281 tataatatag gacttctgaa cctttacctt gaccgttact tgcatttaaa tgtaactcta
     5341 taagtaaatc atatcctcca ctattaactc taggtatttt ataagacttt tcttcattct
     5401 tagttttaaa ctgcttttct gggcatatta ttacatctac cttatgccct tcttttctaa
     5461 atgtatctgc taatactggt gcaagagatt tgttgtattg atactcgtta attactccat
     5521 cagcagaagt gcatgctcca ctttttaaaa tactgtgtcc tactgtaata catattttca
     5581 ttatttatta cctcccttaa tatttaattc gtctgtcatt gtatctagta aattgcctat
     5641 tttctccttt aattttttgg gaactggtaa tccacataag tacatatttt ttaatatact
     5701 tacactttca tacaaaatga atagaataga gaaaaattca gatattccaa gatgatttaa
     5761 tctcaagaaa tcaatccaat cttgtggcaa cataaataaa aagttaaact ttgtaagtat
     5821 atcaactact gctagaaaaa atatacatgc tatcattgca acttttctta ttcctccatt
     5881 tattccaaaa cttgaattaa actgatgtgt ttttattgct cttaagcaac ctaacagtgt
     5941 atcaaatgct attgctaata ttactaattt tataaatata tttgttgcta aaaaaactat
     6001 tgttaaattc atatttcctc ctaatttttg tattaaaaag gacctagaat tatctaagtc
     6061 ctttaaatgt attaatttat tattttacct ttaaattgtt aacgaaatct gcagctataa
     6121 taaagtatgc tgcatcacat tttgcttctc ccataatccc tggattagaa atgtaacaac
     6181 ttgttgggtt ttgttctagc ccctctagct ttatattttc taccattata aatatctccc
     6241 ccatagtagg tataattaac attagaacta gaatgcaaat tacaattatt ttcttcattt
     6301 tttaatctcc ttttaataga atttttttat tatagataaa aataaaaaat atataactaa
     6361 aaaaggaaat tatttttaat ttatataaaa aaaacctatt ttgttggttc tgctccttct
     6421 actactccac tttctctaat tatataatcc tctactgctt ttctatactc tgtgttagtt
     6481 acgtcatcta attcaaattc tcgatttttt agagggttta atcctccgtt taatatcctc
     6541 tctgctaata ttcttactac aacattattt atattcatta taataatcct cctacttttt
     6601 cattttcatt taataaaact tgattttcta attcttgtat tctctttttt tcttcactta
     6661 catatactgg tatttcttcc aaaattggtt gttttgtttc tatatttatg cctataattc
     6721 tatttttaat ataatctata cttccatatg gaatatcaat ataatctaat tcagttattt
     6781 tattgtgtgg taacacatcc ccacaggact cacctgtttc aaaaattatt ttgcctgttt
     6841 ggtcatatat tactctgttt ggaacattca tatttttttc tccttcttaa ataaatttaa
     6901 tagcatacca tttataaata tgtctttgag ttgagtcaaa gttataagct ggaatttgaa
     6961 caccagtatt attaaaataa acatcccctt cggaattatt atagagccaa ccattagcat
     7021 gaaaattttc atcactgttt tttctaataa aagctgtaac gcttgaaaaa tcaacacctc
     7081 tttgtgtagc gacaccacga caagcaaaaa ggaaaagttt ataattattt ttatattcag
     7141 tatcatagta atcaaaatca gcaaagaaaa tgttaggctt aaaatctaaa ttactaattt
     7201 taacccaagt accaggttta aaagttctgt cggttagata tggataagca attttagcac
     7261 tatccgcaaa agaagtgact gttccactag caactttata tttagagttt aattgcgata
     7321 tagtattatt agcttgtgtt aactgattca tcaaatcctg taaactagcg tctccactat
     7381 caaaagactg ctttattttc tctgataact caacaagtgt gttattcaaa cttgcttcta
     7441 tattctttaa tgctaaagtg tttataatac ttgttttccc aactttaaat cctgcattaa
     7501 cctcaactaa ttttgttgat atatcattta aatttacatt ttcgggcagt ggcattatat
     7561 tcttacttat acttaaaact ttttcagcag ttgcattatt gctgtctgta acaactattt
     7621 taagcgtgtg cagtgcatta tcttctaatg tatagttaat tgttttctct gttgttaaat
     7681 ctgttgttat agtttctttt agtacatcat ctataaatac ttctatctta gttaatagtg
     7741 tagggtctgt gtggtcagct ttaaatgttg cttgtgtgga gttataagag gatacggtta
     7801 aaaaaggtaa tgcttgtagt aatgttatta g
//
LOCUS       DAYAOY010000141         7823 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYAOY010000141 DAYAOY010000000
VERSION     DAYAOY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.145"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            430..1731
                     /locus_tag="ACGI6B_003647"
     CDS             430..1731
                     /locus_tag="ACGI6B_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9753056.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1807..2082
                     /locus_tag="ACGI6B_003648"
     CDS             1807..2082
                     /locus_tag="ACGI6B_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFG9753057.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3417..3647
                     /locus_tag="ACGI6B_003649"
     CDS             3417..3647
                     /locus_tag="ACGI6B_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9753058.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3640..4221
                     /locus_tag="ACGI6B_003650"
     CDS             3640..4221
                     /locus_tag="ACGI6B_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9753059.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4800..5477)
                     /locus_tag="ACGI6B_003651"
     CDS             complement(4800..5477)
                     /locus_tag="ACGI6B_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9753060.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5666..7030)
                     /locus_tag="ACGI6B_003652"
     CDS             complement(5666..7030)
                     /locus_tag="ACGI6B_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9753061.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7158..7679
                     /locus_tag="ACGI6B_003653"
     CDS             7158..7679
                     /locus_tag="ACGI6B_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9753062.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3062 a          885 c         1343 g         2533 t
ORIGIN      
        1 aattggtagt aaattatatt aagtaagata ttactatcag gtagtgaatt atattgaacg
       61 atatattaat aattggcagt aaattatatt aaataagata ttaatatcga gtaatgaatt
      121 atattgaacg atatatcaat aattggcagt aagttatatc aaataagata ttactatcga
      181 gtaatgaatt atattgaaca atatattaat aattggcagt aagttatatc aaataagata
      241 ttgctattat gtagtaaatt atataaaaca atgtatcaga ataaatagta tattaataat
      301 ttgtagtata tcatgacgaa taatacagtc ataacaaata gtgagtcata acaaataatg
      361 tatttataat tgataatgta tttaaaaatc aaaatataaa aacttataag gaaagagaga
      421 ttaatttata tgggtaaatc aaaagatgtt gaaatagaga agtctatttt aggcacaatt
      481 cttttagaca ataaattaag ctataagcta gatgaattaa atgaaaacat gtttatgaat
      541 gatatatgtc ttgagatatt taaaattatg aaagaactta aaaaagaaaa catagtgatt
      601 gatgtggcca ctgtgaaatc aaagatagat agaaaatctc tagcaataaa aacaagtgat
      661 gtgactaatc taattacttg gggtcagaat tttggtcttg atggtcatat taaaatttta
      721 aaagaaaatt tagctagaag aagcataaat caaaactgcc aaaacctttt acacagccta
      781 aaccttggag aaaatatcga tacttgtatt tacaaatttg aaagtaatat aaaggagatt
      841 ttggacaagg atacttatga aaatgatgat gtaaatagta tagcagggaa ggtgcttgat
      901 tttttagaaa acaaaaaaga tattggattt aaatttggta taaaattact tgataccact
      961 ataggaggtc tttttaaagg agagctaacc acaatagcag ctaaatcggg ggttgggaag
     1021 actgctttgg cacttcaaat aatgttaaat tcatttaagc aaggtaaaaa aactttattt
     1081 ataagtagag aaatgaccag tgagcaagta tttatgagaa atatatgcag agtaactggt
     1141 gtaagtacaa ggaatatgaa atctaaagaa atagatgaaa atgattggaa acttattgta
     1201 aatgcgatag gtgatttaag cgaaaataac ttaatttata tcaatgataa gatagataca
     1261 attagtgcaa ttagaaagag gataaggcag gtaaagccag atttattaat agtggattat
     1321 gtgcaactcc ttacatctca gaaaagcatg gataagagag aaagggaggt agctactttt
     1381 tcgagagaac ttaaaaatat gactttagat tttaatatac ctgtaatcca attaagtcag
     1441 ttaaatgatg aaatgaaaga ttcaagacct tatggagata gacctatgag ggatagcaaa
     1501 gccatctacc acgatagcaa caatgtggtt tatatacatc aattaaaggg gagtgattat
     1561 gaagaagcag tgagagatat tggagaaagt gaggaggcag ttagagcatc agaatacagg
     1621 ggaattaaga tggttgattt aatagttgcg aagtgtagag atggacagac taggcataag
     1681 catttttgtt actttgggga taagttacat tttcaagaac taaattatta ggtcaataaa
     1741 gtagtaataa caagcttatt aaaagtcggg aaatagcaaa tagattataa aatatgaggt
     1801 gaaaatatgt tggaattaag aaaaattata gaattacttg aagaacacaa gatagaagta
     1861 agtattccaa aggagttaca taagtatagt ttattggaat accttgaatt gtgtcaacaa
     1921 agtgggggaa catattttta tattaaaaca ctggaagggg ctttaaaaga ggctagaaat
     1981 gagcttatga ttaaattgtt taatgggaaa aattatatgg aattagcgag gatgtttgaa
     2041 atgagtgtta ttaatgtcag gcatattgtt agaaaaaaat agatattttt ggagaataca
     2101 agtgtgatat acttctgaaa tagaagttat tataagagga gtataagtgt gattaacaac
     2161 atgattttgt gaataaaatt gaggatatat atgagttaaa tgtgtgatta ataaggtaag
     2221 attgtgtata agattatgag tatttaaaag gaggggctat aattaatagt gtagagttgt
     2281 gtgtttgctt aagattatat aataatttaa tagattaaac tatactaata aggtaaaatc
     2341 gtgtgtttag ttaaagctta ttataaaatc ttgatatgtg attaatgatg caatatgtga
     2401 ataaagttaa gattataata gatttaacct gtggctaata gaataaaatt gtgaataaag
     2461 ttaagattat aataggttta aattgtgact aatagagtaa gagtgtaaat agagataaac
     2521 ttcaatatga gcttaaccta tgattaatag agtaagaatg taaatagaga taaacttcaa
     2581 tatgagttta acttgtggct aatagagtaa gagtgtaaat agagataaac ttcaatatgg
     2641 gtttaacttg tggttaatag agtaaggtgt aactaaagat aaacttcaat atgagcttaa
     2701 cctatgatta atagagtaag aatgtaaata gagataaact tcaatatgag tttaacttgt
     2761 ggctaataga gtaagagtgt aactaaagat aaacttcaat atgggtttaa cttgtggtta
     2821 atagagtaag aatgtaaata gagataaact tcaatatgag cttaacttgt gattaataga
     2881 gtaagagtgt aaatagagat aaacttcaat atgggattaa cttgtgatta atagagtaag
     2941 agtgtaacta aagataaact tcaatatgag cttaacctat gattaataga gtaagaatgt
     3001 aaatagagat aaacttcaat atgggtttaa cctgtggtta atagagtagg gtgtaactaa
     3061 agataaactt caatatgagt ttaacttgtg gctaatagaa taagattgtg aataaagtta
     3121 tagttttaga tgggtattaa cctgttacta atagaatatg attaggtgta aaattataga
     3181 gctgcataag aaaatattat agatatatta aatattagat aataaatatt tatatagata
     3241 cacaaaagaa aaggcgatat gaagctaaac ttaatgactt catatcgttt tttatttgaa
     3301 actcctttgt atttagagtg tgatttttaa tattgaggtt ttatgtgatt aagtcacaga
     3361 taaaaagtaa gtatcttgat ataattctta tagattaata atatagggag gttattatgt
     3421 ctaataaaaa aaagaagtat gcaaatattt tagcaaatga gtgcgttgct tgtggtagct
     3481 gtataaaagc atgtccaaga agtgctatct cagttccatg tggcattagt gcaaaaatta
     3541 atagagattt atgtgttgga tgtgggattt gtgaaaagat atgtccagca tctgtaattg
     3601 agattataac aatattaaaa gaggaagaag gtaagtgtca tgaataataa aaaacattgg
     3661 tatgactatt tatggatttt ttcttcaata tatttgatat tgggatttgt aaatattatt
     3721 ttcgcttgga ttgggctaat ttgtttcttt gttccactag ctatttctat tgttaaggga
     3781 aataaagctt actgtaataa gtattgtgga agagggcaac tgtttaattt gcttggaaat
     3841 aaattgaagc tgtcaagaaa tagagatatt cctaaattta ttagaagtaa gtggtttaga
     3901 tatgggtttt tgacattttt tatggtaatg tttgccaata tgcttttttc aacttattta
     3961 gtgtttgaag gaagtagaaa ttttaaagaa gtagtgactt tgttgtggac tataaaaatg
     4021 ccttggcaat gggcgtatca tggaacttta gtttcacctt gggtggtaca gtttgcattt
     4081 ggtttttata gtgttatgct tacatcaact gttcttggtt tgattactat gatattgttt
     4141 aaacctcgtt catggtgtgt ttattgccct atgggtacta tgactcagat gatatgtaag
     4201 gcaaagagta agtctgtata gaaaggcctt atgtgatatt tacagttttg ttcaagtaga
     4261 gatgagtagt aggtttgaat atatatgaca aatatatgag tataggggca tagatttagt
     4321 ttgtgtaagg taaactgatt tatgcttctt ttttatgtct tagattattg gctaagtgga
     4381 aacaatgaat ttagatatta agaattattc agttatttga agaaaataga ataataatta
     4441 acattatatt aatggatatt cattacatat aagccagtta atagcaagat ttatttaata
     4501 tgatataggt caagtattta aactaaataa ggtcatgaca attaatataa gtatagatat
     4561 aaaaagatta gaagttataa cttataaaag gtattctata taaatagtat ggggaaaata
     4621 gtcaaattaa cttattagaa acaaaaaata tacataaaaa ataaatgtaa atatcaaata
     4681 agatactata tcaaaataga tataagtatc ttttttatta ttgaaattat gaaaatatat
     4741 ttgattttac acaaaaaaga gtgcctcatg aactaaaaag ttcattttga gacacacttt
     4801 tacagtgtca aagaacgaag tttatcctta tccaatacct ttataccacc acgagataaa
     4861 gacacaatac cttctctagc aaaatatttc aacattctcg atacaacttc tctggcacta
     4921 cccatatact tagcaatttg ctcatgagtc atatttatag tgctactgcc atttttagct
     4981 atctcatcaa ttaaaaaaga tgccaaacgc ttatcaaaac tcataaaaag aatttgttgc
     5041 atagcccaca taacatcaga aaaacgttca gtagctaatt tatatgaaaa taactctgca
     5101 tgaatatttt ctgtagataa ttttgcaaaa acagaggaac taatctgtat aatatcgcag
     5161 tccgtttcag catctacata gacatcaaaa gttattgttt ttagaataca ggaagctgat
     5221 aaaatacata catctccatc atcaagtcgg aacagtgtaa cctctcgacc ttcatctgaa
     5281 agtatgtatg tacgcactgt tccagatttt ataatcaaaa taccaataca gtcactatca
     5341 ccacaatgta ctggattccc ttttttgtaa tgcgaggtat ttgctgaaga aattaataat
     5401 tctttttgta agtcagtaag tttatcccaa aaaggcaagc tatgtgtaag tatatcaatt
     5461 tcattttgtg aaatcataca atatctcctt tcttaaattg ttataatccc atgatacaag
     5521 tgaatagata aattgacaag tagtttgtta agaggattat ttagtcagat agtaatagta
     5581 tgatttatgt agaaaaaata tgataatata tattcttgct attaatgaga aagtaatatg
     5641 ttttgatgat agtaggtttg tataactaat gcataccgtt tatttttaaa agattaagtt
     5701 ccttatgtaa ccttatagac ataaaaccag caattatagc agccatcaaa tcagctattg
     5761 gttgtgcata taagatacca tttaacccat agaatacagg aataatcaaa attataggga
     5821 taaaacagac accttgtcta cacataccta aaaatccacc ttcctttgct tttcctagtg
     5881 cgaggaataa tgtagaatac actgtataga atccaaatag cataaatgaa aacccatttg
     5941 ccctaagagc tttactacca ttttctatta ataaaatgtc accacttgta aattgtgaaa
     6001 ttatagattt tgggaagagt atcaatataa gaccaagcac tacacaaaat actgtagtcc
     6061 ataatatgga agtttttatt gctatatgta atctatcgta gttctttgaa ccaaaattat
     6121 aaccagcaat tggttggaaa ccttttataa aaccaaatac aacaagacta cctaaagacg
     6181 ttattcgtgt tacagcaccc attccagcta tcacagaatc accatacttt gtagcctcca
     6241 tattagttaa tgtaatagca agactagtca ataattgaaa tattaaggta ggtatgccaa
     6301 tttttaggat ttctgaaaaa atttcttttg aaaaacagca ttcgttaatt ctaaaactaa
     6361 atatactttt cttgcttaaa atatatccta cataaacaag tgttgaagca atctgtgaga
     6421 tagctgttgc aatagaagca ccccttaccc ccagaccaaa agtataaatg aatatcgggt
     6481 ctaaaatcat attaagcaaa gcacctaata acatagcaca catagttgtt ttagcagctc
     6541 cttcacttgt gacaatatta gtcatagtga cattgaatat attaaaaata gatgacacga
     6601 cataaatgct agcataagat attgcataag gcataatact ttttgtagct cctaacaatt
     6661 ttaaaattgg attgagaaaa atcataatca caataataac taatgtaccc actataatac
     6721 tactgtaaat agatgtactt accacttttc ctgctttttc attatcacca tggcctaaaa
     6781 gtctggcaac atatgaagca gctccatttc caaacaataa tcctaatccc acaacaagct
     6841 gacctaatgg aaatgcaacg gatatagctc ccatttgact tgttccaagt ccacccacaa
     6901 agtatgcatc aaccaaattg tataatgcag ttatcatcat acctatcatg gtaggtaatc
     6961 ccatagataa caatgctttt gagattgggg cagaccctaa taattcaatt cttttgttac
     7021 tttctttcat acttaacact cctcttctat tgacattaag tctaaattat aatactataa
     7081 attatagtaa gtatggaaaa tagtactata atttatagta tttgtcaata gtaagaaaac
     7141 taaaggagat gatttttatg gctacaatag atttaattgt attgggaata gtaaagcaac
     7201 aacccaagag tgcttatgat attcaaaagg cagtggaata caggaatata tcacgttggg
     7261 ttaaaataag tacaccatca atttataaaa aagtaattca attagaacaa aagggatata
     7321 ttgaaagtaa aactgtgaag aatggaaaaa tgccagaaaa agcagtttat tcattaactg
     7381 aattaggtga gacacatttt ttagatttaa tgtttgatac atctagcaaa ccaataaata
     7441 ttcttttaga ttttaatgct gttatagtaa atttaaatag ccttacacca gaaaaacaga
     7501 aattatgtct caataatatt gaagagaatg ttcaagagtt aaagaaaact attgaggaaa
     7561 atataagtat aaagacagaa gtaccagaca cagcaaaagc agtgttggag caacagttgg
     7621 tgctagcaca agcattggaa acctggataa attctttaaa agataatatg ggaaattaga
     7681 attagagaag ctaacttaat aatcattaaa caaaaaccag catatttaat aatgagatat
     7741 tagaaaaaat aaaacaaatg atataaactg aaaagtatat ttaaaataaa gctcacttaa
     7801 ttgagggaga atgttaaact ttt
//
LOCUS       DAYAOY010000142         7598 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYAOY010000142 DAYAOY010000000
VERSION     DAYAOY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.146"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1096
                     /locus_tag="ACGI6B_003654"
     CDS             <1..1096
                     /locus_tag="ACGI6B_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HFG9753063.1"
                     /translation="GAGGGGSGYVLTKDSYKPTGYTPTSEYYFDNVVMESGGNTAGAY
                     GYAKITLLQALPFLTVSSYNSITATFKADHTDPTLLTKIEYFIDDILKETITTDLTTE
                     KTINYTLEDNALHTLKIVVTDSNNTTAEKVLSISKNIMPLPENVNLNDISTKLVEVNA
                     GFKVGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTI
                     SQLNSKYKYATGTAYARENSSLIACVYDPNTSHTVTETSPYWLDLNGIGFIPDIFFAE
                     CEYEPHSNAFYKYFVFAIKNTFPISNNTGFVVNIAFNKEYGDESFKLRGALYTLGKRH
                     VSMDNNGVRVPSLSTLNDFRAYKWHAIKFK"
     gene            1110..1403
                     /locus_tag="ACGI6B_003655"
     CDS             1110..1403
                     /locus_tag="ACGI6B_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753064.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGN
                     IDYSKQYIESINPITKEPILKDIPIYLSEEEKRIQELENQILLNENEKVGGLL"
     gene            1403..1585
                     /locus_tag="ACGI6B_003656"
     CDS             1403..1585
                     /locus_tag="ACGI6B_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753065.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNRPFELDDVTNEDYRKAVED
                     CIIKESGVVERVETTK"
     gene            1613..3514
                     /locus_tag="ACGI6B_003657"
     CDS             1613..3514
                     /locus_tag="ACGI6B_003657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753066.1"
                     /translation="MIYKDLLKKVNDIENLKEVDNIKCSTDTGEYTIENSKKGYLTNF
                     NVEGKTLIDLWGKTSSDFSLWKATFVDGKINILTENDIRYSNFFTINYTSYKPDTIYT
                     IIVDVDKNTLPSTSGIYIHSLGEENSVFIPNLTNIAIPGGVIGKFKYTFTTISDLNDC
                     NVVLRSVLDNDTLTSGYEISLKITILEGDYTDINIDYSNELLSVGQRDKIEFLSYQYS
                     GINIFNKNADFKDNYILQYLSGEELISEASNHKYTLDYIEIEPDTEYTFYNCSRNICW
                     YDINKNFIPASLNERIIGDKDIFYIARSPKNAKYLRVTIIKDLHDNGNKTIITKGNKY
                     DKKTIPYTLRSLPNGVKDEIVYKNDKYYLIKRCEEHTYTDIGNLNLSHVYDNTLQFMG
                     TLTPQAVVDSLDTAYVLCNNLNGKSRNDFNNNDIEGCSTTGSGDIAFKILKSKLTTQD
                     GNGFSEWIKNNPITIIYQLSKPQEIELTSLNLEQYNNQTKFICNTGIVIPDISFESTQ
                     NLGSHIEVIRNNIKNYNVRTDFPFSINFLNGWQPYLGYASSVGNYCTNNNLVTINATI
                     NGGITTAGTIIGKIPSKYAPRKGIIVIFQTTDGKYYNGIIRTNGEIEIYYNDITYRSW
                     LYLYVNYLI"
     gene            3529..3849
                     /locus_tag="ACGI6B_003658"
     CDS             3529..3849
                     /locus_tag="ACGI6B_003658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753067.1"
                     /translation="MDIEKKIEILIEYGFENDLNNIDNYFIAEGKKIFTPVIKNGELI
                     KTGEQVYNEWIELQNNPPKPSIEEINADKITILIENQKQQDSLLVDNAYRIAMLELNT
                     NNVL"
     gene            3875..4045
                     /locus_tag="ACGI6B_003659"
     CDS             3875..4045
                     /locus_tag="ACGI6B_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753068.1"
                     /translation="MYNILKRMIEQKNYETREELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTLVEPII"
     gene            4117..4941
                     /locus_tag="ACGI6B_003660"
     CDS             4117..4941
                     /locus_tag="ACGI6B_003660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9753069.1"
                     /translation="MVILNIKTLTIHAGHNPDNKIGSGAIGNIKESTEARNVLKELLP
                     LAQKECKVYDCTCNNGTSQSDILNKIIAKCNSYNTDLNVSIHFNSGGGRGVEVLVYNL
                     NDKETVEIASRICKKITETYHAKGDKDFKNRGVKEKKTLAFLRRTKAKSILVECCFVD
                     TSDTKKYNTKDMAIDIYEGIFNKSVAGKPQDNKVKYAIVYEGDVDKVIAQLMAMNYKT
                     NEVSVCDLKNYVPGHCENLYVIGGASSKIKTSERFTKLQGDDRWATLHKVLDFIGK"
     gene            4953..5570
                     /locus_tag="ACGI6B_003661"
     CDS             4953..5570
                     /locus_tag="ACGI6B_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9753070.1"
                     /translation="MNILDKSTATLEQVFSYLDTLKNNSNPPHFLCRAIVPIIYKEAE
                     KKGVNPVIAIAQAFVETGYFNFGRVLNPSYCNVCGLKGNKGGGDLDPTAHTRFNCWED
                     GVSAFIDHLALYAGAKGYPKYNELVGKVEYKVNGTTLDPRHFSYLHGEAKTVESLSGK
                     WCPDTNYGQNIINICNKISSMKVENNDIKLEQIKTKVKELNEILG"
     gene            5582..5833
                     /locus_tag="ACGI6B_003662"
     CDS             5582..5833
                     /locus_tag="ACGI6B_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9753071.1"
                     /translation="MDIMQFIPENLIMLIGGLYILGTFIKESNIKNKYIPFILLVIAM
                     ISSCLFMKELSIEAVFEGILCWGASIGINQIQVQNRKDK"
     gene            5834..6145
                     /locus_tag="ACGI6B_003663"
     CDS             5834..6145
                     /locus_tag="ACGI6B_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753072.1"
                     /translation="MLSKEMVELLSQYGYTAILLMVLMLWLGKYLEKNRQLEQDDRKK
                     ERAYFSREIKEQRTLFGNTIDKFDDKLDKFAEALNTNNSRLERVETDITKIKDKLETR
                     D"
     gene            6170..7114
                     /locus_tag="ACGI6B_003664"
     CDS             6170..7114
                     /locus_tag="ACGI6B_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9753073.1"
                     /translation="MKYLAKQRSKRISINGEPNPENIKLWKQYKRAKQIAGKSEKTIY
                     NYECDIMQFFKFLNIECFDTLLTDINEEEIEAYIGYCMEQGNNEKRIRRRISSISSLL
                     SFLKKKRKITDNWCEMIERPGTGEEVQKRTFLTEEQLRKLKEKLSEQDNLQLEVYINL
                     GLATMARSNEISQFRWDNIDLDNRWIHGITAKGGVSRDFRFSEEVQHLLIEWKDYLSQ
                     NNIDYPYIFFTRYGGKYNQVNSNVLSSWVKKAFRLIGIEGGYNHDLRHSMSNLLKDRG
                     VPITTVSKLLGHSGVDVTINHYTIENKNKLAEEYDKFM"
BASE COUNT         3176 a          815 c         1274 g         2333 t
ORIGIN      
        1 gggagcaggt ggaggtggaa gtggctacgt attaactaaa gatagttata agcctactgg
       61 ctacacacca acatctgaat attattttga taatgttgtt atggaatctg gtggaaatac
      121 tgctggtgct tatggttatg ctaaaataac attactacaa gcattaccat ttttaacagt
      181 atcttcttat aattccatta cagctacatt taaagctgac cacacagacc ctacactatt
      241 aactaaaata gaatacttta tagatgatat actaaaagaa actataacaa cagatttaac
      301 aacagagaaa acaattaact acacattaga agataatgca ctacacacac ttaaaatagt
      361 tgttacagac agtaataata ctacagcaga aaaagtatta agtataagta agaatataat
      421 gccactaccc gaaaatgtaa atttaaatga tatatcaaca aaattagttg aggttaatgc
      481 aggatttaaa gttgggaaaa caagtattat aaatacttta gcattaaaga atatagaagc
      541 aagtttaaat aatacacttg ttgagttgtc agagaaaata aaaacaagtt ttgatagttc
      601 agacgctagt gtgcaggatt tgatgaacca gttaacacaa gctaataata ctatatcaca
      661 gttaaattct aagtataaat atgcaacagg aactgcttat gctagagaaa attcatcctt
      721 aattgcatgt gtatatgacc ctaatacttc tcatactgtt acagaaacga gcccttattg
      781 gcttgattta aatggaattg gatttattcc tgatatattt tttgctgaat gtgaatatga
      841 accacattcg aatgcttttt ataagtattt tgtttttgca attaaaaata cttttcccat
      901 ttctaataat actgggtttg tagttaatat tgcttttaac aaggaatatg gtgatgaatc
      961 ttttaaatta cgaggagctt tatataccct tggcaaaaga catgtttcca tggataataa
     1021 tggagttaga gtaccttcac taagcactct aaatgatttt agagcatata aatggcatgc
     1081 gataaaattt aaataaatga ggtgataaaa tgaatagagc aaatagaata atttacgacc
     1141 aaactggtaa aatattactc caaacaggag aagcaacagg agatatatta gagcatgata
     1201 caataacaga attacattat attgatgttg aatatggaaa tatagactat agtaaacagt
     1261 atatagaatc tataaatcca ataacaaaag aacctatttt aaaagatatt ccaatctatt
     1321 taagcgaaga agaaaagaga atacaagagt tagaaaatca aatattacta aatgaaaatg
     1381 aaaaagtagg agggctatta taatgaatat aaataatgtt gtagtaagaa tattagcaga
     1441 gaggatttta agtagaggat tgaatccttt aaaaaataga ccttttgaat tagatgatgt
     1501 aactaacgag gattatagaa aagcagtaga agattgtata attaaagaaa gtggagtagt
     1561 agaaagagta gaaactacaa aataggggtt ttgtgaagag aggtgtttta atttgatata
     1621 taaagattta cttaaaaagg tcaatgatat agaaaatctc aaggaggttg acaatataaa
     1681 atgtagtact gatacaggag aatatactat agaaaatagt aaaaagggct atttaactaa
     1741 ttttaatgta gaaggaaaga cattaattga tttatgggga aaaactagtt cagatttttc
     1801 tttatggaaa gcaacttttg tggatggaaa aataaatata ctaacagaaa atgatataag
     1861 atattctaat ttttttacaa tcaattacac ttcatataaa ccagatacca tatatacaat
     1921 catagttgat gttgataaaa acactttacc gagtacaagt ggaatatata ttcatagctt
     1981 aggcgaagaa aattcagtat ttattcctaa tctgacaaat atagctatac caggaggagt
     2041 aattggtaaa tttaaatata catttacaac aatatcagac ttaaatgatt gtaatgttgt
     2101 tttaaggagt gttttagata acgatacact tacatctggt tatgaaataa gtttgaaaat
     2161 taccatatta gaaggagatt atacagatat taatatagat tactctaatg aactattaag
     2221 tgttgggcaa agagataaaa tagaattttt aagttatcaa tatagtggaa ttaatatctt
     2281 taataagaat gccgatttta aagataatta tattttacaa tatcttagtg gagaggaact
     2341 tatttctgaa gctagtaatc ataaatatac cctagactat atagaaatag aaccagatac
     2401 agagtatact ttttataatt gcagtagaaa tatttgttgg tatgatataa ataaaaattt
     2461 tataccagca tcattaaacg aaagaataat aggagataaa gatatttttt atattgctag
     2521 aagccctaaa aatgcaaagt atctaagagt tactataata aaagatttac atgataatgg
     2581 gaataaaaca attataacta aaggaaataa atatgataaa aagacaattc catatacatt
     2641 aagaagttta ccaaatggag taaaagatga aatagtttat aaaaatgata aatattactt
     2701 aataaaaaga tgtgaggaac atacttatac tgatattgga aatcttaatt tatcacatgt
     2761 atatgataat actctgcaat ttatgggaac attaactcct caagctgttg tagatagttt
     2821 agacacagca tatgttttat gtaataattt aaatgggaaa agtagaaatg attttaacaa
     2881 taatgacata gagggatgct caactactgg tagtggtgat atagcattta aaatattaaa
     2941 aagtaaactt actacacaag atggaaatgg gtttagtgaa tggataaaaa ataatccaat
     3001 aactatcatc tatcaacttt caaaaccaca agaaattgaa ttaacatcac taaatttaga
     3061 acaatataat aatcaaacta aatttatttg caacacaggt attgtgatac cagacattag
     3121 ttttgaaagt acacaaaatt taggaagtca tatagaagtt atcagaaata atataaaaaa
     3181 ttacaatgtt agaactgatt ttccattttc aataaacttt cttaatgggt ggcagccata
     3241 tcttggatat gctagttctg ttggaaacta ttgcacaaat aataaccttg taacaattaa
     3301 tgcaactatt aatggaggta ttacaacggc aggtactatc attggaaaga taccttcaaa
     3361 atatgcacct cgtaaaggca taatagtaat atttcaaaca acagatggaa agtattataa
     3421 cggaatcatc cgtacaaatg gagaaattga gatatattat aatgatataa cttatcgtag
     3481 ttggttgtat ttatatgtaa actatttaat ttagaaagga aaaataagat ggatatagaa
     3541 aagaaaatag aaattttaat agaatatgga tttgagaatg atttaaataa tatagataat
     3601 tactttattg cagagggtaa aaaaatattt actccagtga taaaaaacgg agagctaatt
     3661 aaaacaggag aacaggttta taatgagtgg atagaattac aaaataatcc acctaaacca
     3721 agcatagagg aaataaatgc ggataaaatt acaattttaa tagaaaatca aaaacagcaa
     3781 gacagtttat tagtagataa tgcttataga attgctatgt tagagcttaa cacaaataac
     3841 gtgttataaa attataaaaa atggagggaa caacatgtat aatattttaa aaagaatgat
     3901 tgaacaaaag aattatgaaa ctagagaaga attgcaaact aagttagatg tattttacgc
     3961 tatgaacagg ataaaggaaa gtgaatacac agagttaaca aatttattaa ataaagaaga
     4021 tacactagta gaacctatta tctaaagtat aaaggttctt tttttatgtc aattttagag
     4081 gattgattaa tttcaattct cttttaatta aaaggagtgg taattttgaa tataaaaaca
     4141 ttaacaattc atgcaggaca taatccagat aataagatag gttctggagc aataggaaat
     4201 ataaaggaat ctacagaagc aagaaatgta ctaaaagagt tattaccttt agctcaaaag
     4261 gaatgtaaag tttatgactg tacttgtaac aatggaactt ctcaaagtga tatattgaat
     4321 aaaattatag ctaaatgtaa ttcatataat actgatctaa atgtaagtat ccatttcaac
     4381 agtggtggtg gtcgaggagt agaagtttta gtttataatt taaatgacaa agaaactgtt
     4441 gaaatagcat caagaatatg taaaaaaata actgaaactt atcatgcaaa aggtgataaa
     4501 gattttaaaa atcgtggagt taaagaaaag aaaactctag catttttaag aagaacgaag
     4561 gcaaagtcaa ttttagttga gtgctgtttt gtagacacat ctgacactaa aaaatataat
     4621 actaaagata tggctataga tatttatgaa ggaatattta ataagtctgt agctggtaaa
     4681 ccacaagata acaaagtgaa atatgcaata gtttatgagg gtgatgtaga taaagttata
     4741 gcccaattaa tggccatgaa ttataagact aatgaagtct ctgtgtgtga tttgaaaaat
     4801 tatgttcctg gacattgtga aaacctatat gtgattggtg gagcatccag taaaattaaa
     4861 actagcgaga gattcactaa gttacagggc gatgatagat gggctacact tcataaagtg
     4921 ttagatttta taggcaagta ggaggtaatg aattgaatat attagataaa tcaacagcta
     4981 cattagaaca agtcttttcg tatttagaca cattaaaaaa taactctaat ccaccacatt
     5041 ttttatgcag agctatagta ccaattatat ataaagaagc agagaaaaaa ggtgtaaatc
     5101 cagttattgc aattgctcaa gcatttgtag aaactggata ttttaatttt ggtagagttt
     5161 taaatccttc ttattgcaat gtgtgtggtc ttaaaggaaa taaaggtgga ggagatttag
     5221 acccaacagc tcatacaaga tttaattgtt gggaggatgg agtatcagca tttattgacc
     5281 atctagcatt atatgctgga gctaagggat atccaaaata caatgaatta gttggtaaag
     5341 ttgaatataa agtcaatgga actacattag accctagaca tttttcatat ctacatggag
     5401 aagctaaaac agttgaaagc ttatcaggta aatggtgtcc agatacaaac tatggtcaaa
     5461 acataataaa tatatgcaat aagataagtt caatgaaggt agaaaacaat gatataaaac
     5521 tagagcaaat aaaaactaaa gtaaaagaat taaatgagat tcttggatag gaggaaatca
     5581 catggatata atgcaattta tacctgaaaa tttaataatg ttaataggtg ggctttacat
     5641 attaggaaca tttataaaag aatctaatat taaaaataaa tacatacctt ttatattgtt
     5701 agttatagca atgataagta gttgtttgtt tatgaaagag ctgtcaatag aagctgtctt
     5761 tgaaggtatt ttatgttggg gtgcatctat aggaataaat caaatacaag tgcaaaacag
     5821 aaaggataag tagatgttat caaaagaaat ggttgaatta ctaagtcaat atggatatac
     5881 agctatactt ttaatggtac tgatgttatg gcttggaaaa tatttagaga aaaatagaca
     5941 actagaacaa gatgatagaa aaaaggaaag agcttatttt tcaagagaaa ttaaagagca
     6001 gagaacttta tttggtaata caatagataa gtttgatgat aaattagata aatttgctga
     6061 agcattaaat actaataaca gtagacttga aagagttgaa acagacataa caaaaattaa
     6121 agataaatta gagactagag attaatttct ctagtctttt tataaggaag tgaagtattt
     6181 ggcaaaacaa cgcagtaaaa gaataagcat aaatggagaa ccaaatccag agaatattaa
     6241 attatggaag caatacaaaa gagcaaaaca aatagctggg aaaagtgaaa aaacaatata
     6301 taattatgaa tgtgatataa tgcaattttt taagttttta aatatagaat gttttgatac
     6361 attactaact gatataaatg aagaagaaat agaagcttat ataggttatt gtatggaaca
     6421 gggaaataat gaaaaaagaa ttagacgtag aataagttca atttcatcat tgctatcatt
     6481 tttaaagaag aagaggaaaa ttactgacaa ctggtgtgaa atgatagaaa gaccaggaac
     6541 aggcgaagaa gttcagaaaa gaactttttt aactgaagaa cagttacgca aattaaaaga
     6601 aaagctttca gagcaagaca atttacagtt agaagtttat ataaatttag gattggctac
     6661 aatggctaga agtaatgaga taagtcaatt tagatgggac aatatagatt tagataatag
     6721 atggattcat ggtattacag ccaaaggtgg agtttcaaga gattttagat tttcagaaga
     6781 agtccagcat ctattaatag aatggaagga ttatctatct caaaataata tagattatcc
     6841 gtatatattc tttacaagat atggaggcaa atacaatcaa gtcaattcaa atgtcttaag
     6901 ttcatgggtt aaaaaagctt tcaggttaat tggtatagaa ggtggatata atcatgacct
     6961 tagacatagt atgtctaatc ttttaaaaga cagaggagtt ccaataacta cagtttcaaa
     7021 attgcttggc cacagtgggg ttgatgtaac tataaatcac tacacaatag aaaataaaaa
     7081 taaattagct gaggaatatg ataagtttat gtaaattgaa aggaagtggt atcatggcat
     7141 gtaaaggtcg taaaaaaggt aagaaaaaga gatagataaa taataaagcc ctctagggat
     7201 tactcttaat tgagttttct ttagagggct ttattttttt atatatgttt atataaattt
     7261 ataattatat ataatgctaa accacttaaa atacttttca aaaaaaatga ttttatttga
     7321 aattttttaa ataaaacatc agaaacataa aatacagcac atgctataaa aaaatataaa
     7381 aattcattat taattcctgc caatctaata tgtatgatag taaataatat tataataatc
     7441 aaaaacgtat ctataaaatt tttgtgtttt cattcaatat attaaactta ttttaattag
     7501 gtatctatct caagtctatc ctaatagtat agaatatgta agaaattatt aattaataat
     7561 tcttaactat caataaacta atcttaatta tttattaa
//
LOCUS       DAYAOY010000143         7519 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYAOY010000143 DAYAOY010000000
VERSION     DAYAOY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.147"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            124..633
                     /locus_tag="ACGI6B_003665"
     CDS             124..633
                     /locus_tag="ACGI6B_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFG9753074.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            645..1526
                     /locus_tag="ACGI6B_003666"
     CDS             645..1526
                     /locus_tag="ACGI6B_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFG9753075.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1654..1859
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1985..3256
                     /gene="serS"
                     /locus_tag="ACGI6B_003667"
     CDS             1985..3256
                     /gene="serS"
                     /locus_tag="ACGI6B_003667"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFG9753076.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3577..4032
                     /locus_tag="ACGI6B_003668"
     CDS             3577..4032
                     /locus_tag="ACGI6B_003668"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFG9753077.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4033..4124
                     /locus_tag="ACGI6B_003669"
     tRNA            4033..4124
                     /locus_tag="ACGI6B_003669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4067..4069,aa:Ser,seq:gga)
     gene            4162..4426
                     /gene="ffs"
                     /locus_tag="ACGI6B_003670"
     ncRNA           4162..4426
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGI6B_003670"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4503..6140
                     /gene="dnaX"
                     /locus_tag="ACGI6B_003671"
     CDS             4503..6140
                     /gene="dnaX"
                     /locus_tag="ACGI6B_003671"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFG9753078.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6199..6549
                     /locus_tag="ACGI6B_003672"
     CDS             6199..6549
                     /locus_tag="ACGI6B_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFG9753079.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6562..7161
                     /gene="recR"
                     /locus_tag="ACGI6B_003673"
     CDS             6562..7161
                     /gene="recR"
                     /locus_tag="ACGI6B_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFG9753080.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7302..>7519
                     /locus_tag="ACGI6B_003674"
     CDS             7302..>7519
                     /locus_tag="ACGI6B_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753081.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEP"
BASE COUNT         3060 a          755 c         1389 g         2315 t
ORIGIN      
        1 tcttttttat ttattataaa agttaagatt aaatagaata taaagtcaat attgatttat
       61 aataagataa aatataaaat tatatagtat aggtaaagtt tttatttgga aagaaggtgt
      121 aatatgaatt ctaatgaaag aagaagtaaa ttaatagata ttttaaaaga atctaagcat
      181 cctgtaaaag gtggaacact agctgaacta ttaaatgtaa gtaggcaagt aatagttcaa
      241 gatatagccc ttataagagc tagaggattt gaaataatag ctacacctca aggatatata
      301 atatataatc aaccgtattt tacaaaacaa ataaagtgta aaaatcataa ggattctaaa
      361 gaaatttatg atgaattaaa gataattgtg gatttaggtg gtattataaa agatgtcatc
      421 gtaaatcatc caacatatgg tcagataact gcagaactta atatttattc taaaatggat
      481 atagacaact ttatgaagaa agttgagaca aatgaattta aacaattatc ggttctgaca
      541 gaatgtagcc atattcatac aattgaggca ataaaagaag aaactataga agctatagta
      601 aaagagttaa aggacaacgg aatattatca taaggaggag ttgaatgaat tttttatcat
      661 tggctaagac tgcctcaaca aatagcaata taactaagag aactttagaa aatatgctcg
      721 ataattttag tgataatata ccaaatatta tatatgcaat aattgtattt gtgataggta
      781 tttatatatc taaaatagta agaaggatgg tttcaaagtt tctaatcaaa tatggaatga
      841 gtaaaggtgt aaataatttt attgtatatg gaatatatat atctatgtta tctattatat
      901 cattgataag cttaggtatt atagggatac aaactacatc agttgtagct gtgttaggag
      961 ctgctggatt tagtataggt ttagctttta aagaaatatt atcaaattta ggctctggta
     1021 tgattatttt attttttaag ccatttaata taggtgatta tattcaaggt tcaggtgtag
     1081 aaggtacagt ttctgacata caaatattta gcactgttct taagacgcca gataataaaa
     1141 ctataattat acctaatttt caactgacaa gtaataatat aattaattac acacatcaaa
     1201 acaaaagaag aatagatttt tcatataata tttcatatga tagtgatata gatgtagtta
     1261 agtctatatt aaatgaaatt tttacaaacg aaaaaagaat attaaatgat ccaaagccta
     1321 taataggttt aaactctata ggaaataaca ccatgcaaat agttgctaga ccttgggtaa
     1381 aaactgatga ttactgggat gtatattttg atgtaatgga aaaggtaaaa aataaatttg
     1441 atgagaatga tataaaagtt ccatttgttc cttcttcatt attatttaat aatacaggta
     1501 atctaaatac aatggataaa agataaggtt ttaattaaat agaaaaaatg aatttaatat
     1561 aaaaaattaa agaaaattct aaaaaaattt gaataatagt attgaaagat gttaatatat
     1621 ataatataat gattatcaaa taaaacaatt agttaaaaag ctgtgaagga aaatagtaaa
     1681 aaacagattg tattcagaga atgagtggat ggtgaaaact cattacaatg gtttttgaat
     1741 ctactctaga gcttctagtt aaagctagac gtttacctac gttataggtc tgagaggatt
     1801 atacatttta gtatagtcaa ttagggtggc aacgcggata aaagattttc gtccctttta
     1861 tagggtaaac ggaggtcttt ttttattttt aaaatttgtt taaactaaga gtttacaagt
     1921 ataatgtttg taaattaagt tggaattata acttatatta tcaaaattat taggaggaat
     1981 taatatgtta gatattaaaa gaataagaga aaatctagat gatatcaaaa aagctatgga
     2041 aagaagaggt gaaagagaat ttgaccttga tgctgtagtg gaattagata ataaaagaag
     2101 agaaatactt caagaagttg aagttatgaa aaatgagtta aatgtaaatt ctaaaaaaat
     2161 acctcaatta ataaaagaag gaaaagatgt aacagaagaa aaggcaagat tgaaagagtt
     2221 atcagataag ataaaaggta ttgatgagaa agtaaaagaa gtagaagcaa aaatggaata
     2281 tacattaatg agaataccaa atgttcctca tccagaggta ccacaaggtg aaacagatga
     2341 agacaatgtt caaataagaa cttggggaga acctactaaa tttgattttg agcataaagc
     2401 gcattgggat ataggtacag gtctaggtat tttagatttt gaaagagcag ggaaaataac
     2461 tgggtcaagg tttactttat ataggggatt aggagctaga ttagaaagat ctttaatgaa
     2521 tttcttttta aatactcata cagctaagca tggctatacg gaagtcttac ctccatttat
     2581 ggccaataga aatagtttta tagggactgg tcaacttcct aaatttgaag aagatatgtt
     2641 taaaatagaa ggattagagt atttcttaat accaacagct gaagttcctg ttactaatat
     2701 acatgctaat gaaatattag atgttgcaga gcttccaatt aaatactgtg catatactcc
     2761 ttgttttaga tctgaagcag gttcagcagg aagagataca agaggtttag ttagacaaca
     2821 tcaatttaat aaagttgaat tagttaaatt tgttaaacca gaagattctt ataatgaatt
     2881 agaatcatta actcatgatg ctgaaactat gttacaaatg ttaggtcttc catatagagt
     2941 tgtaaaaata tgtacaggag atttgggatt tacagctgca tttaaatatg atttagaagt
     3001 ttggatgcca agttacaata gatatgtaga aatatcttct tgttcaaatt ttgaagattt
     3061 ccaagcaaga agagcaggaa taagatttaa aagagataaa aaatcaaaag cagaatatgt
     3121 tcatacatta aatggttctg gattagcaat aggaagatgt ttggctgcta tattagaaaa
     3181 ttatcagcag gcagatggtt ctgtagtagt tccagaagaa ttaagacctt atatgggtgt
     3241 tgatgttata aaataaattt atattaattg tatatataga gtatgctttg atgaagagaa
     3301 tatattatga gtaaacaata aataaaaaaa ctatgaaatt attataaata ttaatttcat
     3361 agttttttta tgttaattta cacagcagaa ttttcatcat aaaacactaa atatttttat
     3421 aaaacactct tgtatgaata tctaaaaatt attttagaac taattttaaa aaaatatagc
     3481 tatatatagt aataattaaa taaacaaaat aaaattaagt tgaattataa aatgaattat
     3541 gataagataa gtttgttata agttttggga ggaattatgg aaagttcatt ttatatgaaa
     3601 gaagcattaa aagaagcata taaagcatat aataaaaaag aaacaccaat aggtgctatt
     3661 atagttaaag ataatcaaat aatagctaga gctcataacc ttacagaaac gttaaaagac
     3721 tctacggcac atgcagaaat tttagcgata aaacaagcat cagaaaaatt aggtggttgg
     3781 aggttgactg attgtgattt atatgttaca atggaacctt gcataatgtg tagtggcgca
     3841 atagttaatt ctagaataaa aaagcttata atagggacaa gacatgtaaa aaattcatat
     3901 atagaaaaac agcatgagtt taaattagat tattttaata ataataatgt aaaagtagct
     3961 tttgatgttt tgcaagaaga atgctcgatc atattgcaag aattttttaa ggcattgaga
     4021 aaaagagatt aaggagagat ggtcgagtgg tcgaaggcgc tcgcctggaa agcgagtata
     4081 tgggtaaaac tgtatcgtgg gttcaaatcc cactctctcc gccagaaaca atgaaagtaa
     4141 ttaaatatgg ttgtaatttt gctgtactag atggggaggt agcggtgccc tgtaacctgc
     4201 aatccgctat agcaggattg aattccatct aaaggcttat tttctgtagg gccgccctaa
     4261 ataagtggtg ttgatgctta ggtcctgcgc aatgaaagct catgaacccc gtcagatccg
     4321 gaaggaagca gcggtaagtg atcacttttg tgtgccgtgg gggtgcctaa gttgagctaa
     4381 ctgttttggt aacgcctatg gaattaagtc gatagatggt gtacagcatt taatatataa
     4441 aagactaatc ccttagataa tgagggattt ttttattaat tttgctaaag agaggtaaaa
     4501 agatgcataa agcattatat agagcatata gaccacaaaa atttgaagat gttattggtc
     4561 aagatcatat tattaagact ttaaagaatc aaatatatag tgataatata ggacatgctt
     4621 atttgttttg tggaacaaga ggtactggta aaacatctac tgcaaagatt ttttcaagag
     4681 cagtaaactg tttgaataaa ataaatgaag aaccgtgtaa tgagtgtgag atatgcgaat
     4741 ctgtattaaa agataataca atggatgttg tagaaataga tgcagcttca aataatagtg
     4801 tagatgatat aagagagctt agagaaagtg tgaaatattc tccagccaat gctaaatata
     4861 aagtatatat aatagatgaa gttcatatgt tgtctcaagg agcttttaat gcattattaa
     4921 agaccttaga ggaacctcca tcatatgtaa tttttatatt agcaactaca gagcctcaca
     4981 aaatacctgc aaccatttta tcaagatgcc aaagatacga ttttaaaaga gttacagtta
     5041 aagatatgac ccttagaatg aaaaagattt gtgaagatga aggaattgat atagatgata
     5101 aagctcttaa tttaatagct agaaattctc aaggagctct tagagatgca cttagtatat
     5161 tagaccaatg tatgtctttt ggagaaagta agatagatta taaagatgtt gttgaattaa
     5221 tgggaagcgt aaatatagaa caattatttg aattgtctca atgtatagta gagcaggaca
     5281 ctaaaaagtc tcttgagata ttaaatgaat ttgtcttatg gggcaaagat ataagaaatc
     5341 ttataaatga cttaattgat cattttagaa atttaatggt atgtaaggtt tcatcagaat
     5401 tggatgaaat aatttcatta ccagaagaaa ctatagaaca acttaaaatt cagtctaaaa
     5461 atatagatat aaatgattta ataaggattt taaatatatt atcaataact caggatgata
     5521 taaagtcatc ttcaaatcca agggttttag tggaaataac cataatgaaa attgctcaac
     5581 caatgtttga tgaaagtaaa gaagctctta taaaaagagt tgagaactta gaaaaaatga
     5641 ttgaactggg gaactttaag tctgaaaaaa ttgggaataa taaagaaaaa gagtatgagg
     5701 tcgatagaga aatagatgta aaacaagaaa atgtagttta tgaagatgta aaaaatgagg
     5761 acgttatact tatagagtca tcttggaaga atatacttaa acaaattaaa aaagataaaa
     5821 aaatgccaat atacgcactc ttaagtgaag taaaaagttt taatgtatat tctaatatgt
     5881 tgtatgttat atttgatgat aaatttgatt ttgcaaaaac tagactgagc tctcaagata
     5941 caataaatta tttagaaaaa acaataagag atgttttaaa tagaagtttt aatgtaaaaa
     6001 tagtactgac atcagaagtt aaagatataa atcttgaagt taaagagaaa aaggacattg
     6061 gtgaggaaat attaaagaac atagtgagtg aagagatatt agaaataaaa gatagtattg
     6121 atgaaaatga gagtaaataa taaaatacta ttatgatata ataataaaat gaaaataagt
     6181 ttaggaggaa tagtgtttat ggctaaaaaa ggttttggag gcggaatgat gcctggtggt
     6241 ggaaatatga acaacttgtt aaagcaggct caaaaaatgc aagaaaatat gcaaaaagct
     6301 caacaagagt tagaaagtaa agaagtagaa gcttcagttg gtggaggagc agttactgta
     6361 aaagttaatg gtaaaaaaga agttatagat ataactatca agccagaagt agttgatcca
     6421 gatgatatag aaatgctaca agatttagtt ttaagtgcag taaatcaagc attgagaaat
     6481 attgatgata tacaagcaag tcaaatgagt aaggtaactg gaggtatgaa tatacctggg
     6541 ttattctagt aggtggtatt tatgcaagtt tatacaggtc ccataacgag acttatagag
     6601 gaattttcaa agcttcctgg agtaggaaga aagactgctc aaagattggc ttttcatatt
     6661 attaatatga atactaatga tgtggaagct ttatctaaag cgattataga tgcaaaaaga
     6721 gaaataagat attgctcaat ttgttgtaat ataacagata cagacccatg tagcatgtgt
     6781 tcaaataaaa gtagagattc aagtgttata tgtgttgtgg aagatccaag agatgtagca
     6841 gctatggaga gaacgagaga gtttaaagga cagtaccatg tattaaatgg agtaatttct
     6901 ccaatggatg gaattggacc agatatgtta aaaataaagg aacttatcca aagacttggc
     6961 aatcaggaag tcaaagaaat aataatggca acaaatccta ctattgaagg tgaagctaca
     7021 gccatgtata ttgctaggtt agtaaaacca atgggaatca aagtcactcg gatagctcat
     7081 ggtcttcctg ttggaggcga tttagagtat gcagatgagg tcacaatatc aaaagcacta
     7141 gaaggcagaa gagaaatata aaaatcgagg ttaatacctc gatttttgta attttttgag
     7201 ttaaattcaa attaattttg atttagattt attgaataaa tttcttctgt attgtataat
     7261 ttgttgtata aatcatttaa aaattttgat aggggtgcaa catgaaacca attatgaact
     7321 tagaagaaca taatgcaatg aaagaaaaaa gagttaaaaa aatgaaatca aaagataaac
     7381 ctaaatatga tgattttgag gatataagtt tttctaataa gaaaaaaact aaaaaaaatg
     7441 tacgaaataa aaatgctgaa tttagaaata attataaaaa ttatcagtat gattatgaag
     7501 aagactttta tgaaccaat
//
LOCUS       DAYAOY010000144         7510 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYAOY010000144 DAYAOY010000000
VERSION     DAYAOY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.148"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            62..544
                     /locus_tag="ACGI6B_003675"
     CDS             62..544
                     /locus_tag="ACGI6B_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753082.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLENEFCGCKAQ
                     SYEEKTGPTYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIIDMLYPKIKEFELPIFSWKA
                     "
     gene            1067..1744
                     /locus_tag="ACGI6B_003676"
     CDS             1067..1744
                     /locus_tag="ACGI6B_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9753083.1"
                     /translation="MLKILQDKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     QTLKQIRIHGYYIATEKDQEWLDIRAEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            1888..2055
                     /locus_tag="ACGI6B_003677"
     CDS             1888..2055
                     /locus_tag="ACGI6B_003677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753084.1"
                     /translation="MSDGKICVSCQRTIADVATELTIAYMENASLTPEKIAEIYKLFY
                     KNANEAFNGKF"
     gene            2122..2808
                     /gene="terS"
                     /locus_tag="ACGI6B_003678"
     CDS             2122..2808
                     /gene="terS"
                     /locus_tag="ACGI6B_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFG9753085.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKYNVSLSTVKSWATRYWKQKG
                     CNQSKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMVKELKKYESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQRARVEVLKSKINNQESKEDKLDRYF
                     DKLESVIKND"
     gene            2801..4030
                     /locus_tag="ACGI6B_003679"
     CDS             2801..4030
                     /locus_tag="ACGI6B_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9753086.1"
                     /translation="MIDELYHSKQLEVLNFALNNDYFMLINYGAKRTGKTIIDNDLFL
                     LELRRVRKIANELGIKLPQYILAGADLGALQRNVLSELTNKYDIEFKFDKHNRFVLFG
                     VQVCCFGHSKTNDLGRIRGMTSFGAYINEGTVANEEVFNEIKSRCSGEGARILVDTNP
                     DQPEHWLKTNFVDKTDGKVIQSFHYKLDDNIFLSERYRANIKKSTPSGVFYDRDINGL
                     WVSADGLVYQDFNKDIHYISKDKLNDINFVRYFAGVDWGYEHFGAIVVIGEDDKGNLY
                     LLEEHSAQHKEIDYWIDKAKSIKEKYGNIKFYCDSARPEHLAAFKRNGIKAFNANKAV
                     LSGVEAVAKRIKTNTLFVVHENVNLFRKEIFMYAWNKNTGEPIKKWDDVLDALRYAIY
                     TDSLGTGIKVLTPNGRR"
     gene            4017..5429
                     /locus_tag="ACGI6B_003680"
     CDS             4017..5429
                     /locus_tag="ACGI6B_003680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9753087.1"
                     /translation="MEEGDKLELDVIKKLIEQTNSKHSNFVKKADEAEKYYKNENDII
                     RDRSPNNIGKVNTTNNPLRNADNRIPFNWFGFLVNQKISYLFTYPPTFDVGDDRVNSK
                     ITDILGDRYPKEAKTLGKNASIYSKAWLHIWVDDNNNFQYANIDPRQIRAVYSSDLNR
                     KLLAVLREYKKTDDKGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDN
                     KLETQTNVYKHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEV
                     IFVLTNYGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTE
                     KQIYVQGQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRMICRHL
                     GYSPKRVLQTWTRNMIQNDLELAEICSKSVGIISEKTNLKNHPLVDNAEEEEKQIKKE
                     NSQQEYDDLIPNQDGGIDET"
     gene            5419..6954
                     /locus_tag="ACGI6B_003681"
     CDS             5419..6954
                     /locus_tag="ACGI6B_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9753088.1"
                     /translation="MKHNDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEARKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCHCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            6956..7204
                     /locus_tag="ACGI6B_003682"
     CDS             6956..7204
                     /locus_tag="ACGI6B_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753089.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            7275..>7510
                     /locus_tag="ACGI6B_003683"
     CDS             7275..>7510
                     /locus_tag="ACGI6B_003683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753090.1"
                     /translation="MLKLYILSIIVFCTGLYLFKMKIDSNEELIEILKNKKVRRKCNF
                     IFMALFPLLNFILGVIFIISSLLVSNEDMIKNLKG"
BASE COUNT         3081 a          786 c         1388 g         2255 t
ORIGIN      
        1 cttttatata caaatattat tataacataa aaacataaaa aattgatggg agtgtggaag
       61 tatgaataaa aagacactat ttcaagaggt tgaaggtaga ttatataact ataaaaaact
      121 agaaagtcaa ataagaataa aagatatata tattaaaaaa ttagaaaatg aattttgtgg
      181 atgtaaggct caaagttatg aagaaaaaac aggacctact tataatataa gttcaagtgt
      241 tgaaaatgaa gttattaaga gagaagaaga tttaaataga ttaaaagagg ataagaaaac
      301 attagaaatt gaaaaagaaa ctatagaatg tgcattaaca agtctaaata gctttgaaac
      361 agagttcttc aatgaaatgt acatgaacaa tgaaaagata aacatggatt atatgtctaa
      421 tgctatgcac atagatagaa gtcattgctt tagaataaga aagaggatag tttgtaagat
      481 tatagatatg ttatatccaa aaataaaaga gtttgagttg cctatttttt catggaaagc
      541 ttaaaaatga gactattttg agactttttg gagactatcg tgagactttt tattggcaaa
      601 accatgagat aataatattg tggaaatgaa agatttccct ctcaaaacta aataattgct
      661 aggttagttt aaagggctaa tctagcaata tgaacagact aggcagggcg tgaggacgct
      721 gttagttcaa ttctaactat gttcaaaatc tattgataca ctatattaag tatttgaatt
      781 aagattaaaa tctcatacag ttttgtatct taattcagaa atctaaaaaa tcgggtgggg
      841 cttggtaacc tcactcacca tgcaggtagt ggtttaatct aggttcgatt cctagaactt
      901 gctcccttaa taatatgtat cccccattaa aaaggcttag attaatttct aggtcttttt
      961 tatacaaaaa tttaataagt tttagatatc agtaactctg ataacagaaa ataagggtgt
     1021 tcaagttgag caccctttat taataactaa tttaggaggt agtagtatgt tgaaaatttt
     1081 acaagataaa aatgtaaaag taatgtggtc caaaaatgga gaagaagttt ggtttaatgc
     1141 aaatgacgta ggagaggaac taggcatagt aaatattcgt gatacattaa gaaatataga
     1201 tagagaatat aaaaagaaat ttaatgagtc tactgtcgga gattcctaca ctagaaactt
     1261 taaagataaa ttacctaact tcggtactac ttttgttaca gaagaagctg tgtacaatat
     1321 gtcatttaga agtaataaac cagaagcaaa gttatttaca aaatgggtta cacaaacact
     1381 taaacaaatt agaatacatg gttattacat tgctacagaa aaagaccaag aatggctgga
     1441 tataagagca gagggtaaaa aggtaagaaa agattttaca gatgaaatac aagagtttgt
     1501 ttattatgct actagtcaag gaagtaataa acctcagatg tattataaac attttactga
     1561 acttgtaaga aaaaaattag gtataccaaa aggtgtgaaa agggatgaat taaatcaaag
     1621 tgaattgttt gatatacaag cactcgaaag aattatatct atgaaattac ctaagctaat
     1681 agataaagat atgaattata aagaggtata taaaaagatt aaagagttaa tagaaatgat
     1741 ttaaatgact gtcttgatgg atggtctttt tttatataat aaattaaata agtgtattat
     1801 ttagaattat tttgtagttg tcgaatagtt tttgaaggat attgaccttt gaagttgaat
     1861 tatatacttt ataaggaggt gactattatg tctgatggaa aaatatgtgt ttcttgtcaa
     1921 agaacaattg cagatgtagc tacagagctt acaatagcat atatggaaaa tgcttcttta
     1981 actccagaaa aaattgctga aatatataag ttattttata aaaatgcaaa tgaagcgttt
     2041 aatggtaaat tctaaaagat taagaactct aaccagagtt ctttttttgt tcccaaaacg
     2101 acaaacaaac gaggtggtgg tatgaatgaa aaggcagatt tagctcatga agattactta
     2161 aaagggctta agtacaagga aatagctgaa aagtataatg taagtttatc gactgtaaaa
     2221 tcatgggcaa ctagatactg gaaacaaaaa ggttgcaacc aatcaaaaaa agttgcaacc
     2281 aaaaagagag gtgcacccat aggtaataaa aacgctactg gtccaccagg taataaaaat
     2341 gctgaaaaat ttggtttctt ctcaaaatac ttacctgaag aaacccaaga cttaattaat
     2401 gagataaagg ataaagataa atttgatatt ctttgggaac agataacaat acaatatgca
     2461 gcaataataa gagcacaaaa gataatgtat gttaaagaca aggaagaaat ggttaaagaa
     2521 ttaaagaaat atgaaagcac agaaaatggt gaaaagatag agtatgaatt tcaatttgca
     2581 tgggataggc aagcatcttt tcttaatgca cagagtaggg caatgagtga gttaaggagt
     2641 ttaattaaac aatatgatga aatgattcat aaggattgga atttagctac agaggagcaa
     2701 agagcaagag tagaagtatt aaaatcaaag ataaataatc aagaaagtaa agaagataaa
     2761 cttgatagat attttgataa actcgaaagt gtgataaaaa atgattgatg aattatatca
     2821 tagtaaacaa cttgaagttc ttaactttgc tttgaataat gactacttca tgctaattaa
     2881 ttatggtgct aaaagaacag gaaaaacaat aattgacaat gatttattct tacttgaact
     2941 tagaagggtt agaaagatag ctaatgagct aggtattaag cttcctcaat acatcttagc
     3001 aggagcagat ttaggagctt tacaaagaaa tgtactaagt gagcttacaa ataagtatga
     3061 cattgagttt aagtttgaca aacataatag atttgtattg tttggagttc aagtatgttg
     3121 ctttggacac tcaaaaacaa atgatttagg cagaataaga ggtatgactt cgtttggggc
     3181 atacataaat gaaggtacag ttgcaaatga agaagtattt aatgaaatca aatcaagatg
     3241 tagtggagaa ggtgctagaa tacttgtaga tactaaccca gaccaaccag agcattggtt
     3301 aaaaactaac tttgtagata aaacagatgg caaggttatt caatcttttc attacaaatt
     3361 agatgataat atatttttaa gtgaaagata cagagcaaat attaagaagt ctactcctag
     3421 tggcgttttt tatgacagag atataaatgg tttatgggtg tcagcagatg gattagttta
     3481 tcaagatttt aataaggata ttcattatat ttctaaggat aaattaaatg atattaattt
     3541 tgtaagatac tttgcaggcg ttgattgggg atatgaacat tttggagcta ttgttgtaat
     3601 tggagaagat gataaaggta acttatattt attagaagaa cattcagctc aacataagga
     3661 aatagattac tggatagata aggctaaaag tataaaagaa aagtatggaa atataaagtt
     3721 ttattgtgat agtgctagac ccgaacattt ggcagcattt aaaagaaatg gtataaaagc
     3781 ttttaatgct aataaagctg tattgtctgg cgttgaagct gtagctaaaa gaattaaaac
     3841 taatacttta tttgtagttc atgagaatgt taatttattc aggaaagaaa tttttatgta
     3901 tgcttggaat aaaaatacag gtgagccaat taaaaagtgg gatgatgtat tagacgcact
     3961 tagatatgcc atctatacag attccttagg aacaggaatt aaagtactta caccaaatgg
     4021 aagaaggtga taaattggaa ttagatgtaa taaaaaagtt aattgaacaa actaatagta
     4081 aacatagtaa ctttgttaaa aaagctgatg aagctgaaaa atactataaa aatgaaaatg
     4141 acattataag ggatagaagc cctaataata ttggaaaagt aaatacaact aataatccac
     4201 taagaaatgc agataataga ataccattta attggtttgg ttttttagtt aaccaaaaaa
     4261 tatcatatct gtttacttat ccacctacat ttgatgttgg agatgataga gtaaattcaa
     4321 agataactga tattttaggc gatagatacc caaaagaagc taaaacactt ggcaaaaatg
     4381 ctagtatata ttctaaagca tggttacata tttgggtgga tgataataat aactttcaat
     4441 atgctaatat agaccctcgc caaataagag cagtatattc atcagattta aacagaaagc
     4501 ttcttgcagt acttagagaa tataaaaaga ctgatgataa aggaaaagaa tatgtaattt
     4561 atgagtattg gacagatgaa tgttgttata cttatcaaaa taaagatgga aatagtaata
     4621 tcaatggatt ggagatactt aataaattta tagagaaaaa tttagataat aaacttgaaa
     4681 ctcaaactaa tgtatataaa cacaattttg gagaagttcc atttatcgaa tttctaaaca
     4741 atgatttaga ggtaagagat ttagataatg ttaaacacct cattgatgta tatgacaagg
     4801 tttatagcgg ttttgtgaat gatattgaag atatacaaga agttattttc gttcttacga
     4861 actatggagg tgcagactta acagagttct taaaaggact taaagaatat aaaactattg
     4921 atttacaaag tagtggtgca gatgataaga gtgggttaag tacaattaca ataaatattc
     4981 caattgaagc tcgagattca cttcttaaaa caacagaaaa gcaaatctat gtccaaggtc
     5041 aaggtgttga ccctaaaccc gaaaactttg ccaatacaag tggtgtagca ctcaaattct
     5101 tgtacacttt gttagaatta aaagcaggac ttatggaaac agagtttaga ttagggtttg
     5161 ctaaactagt aagaatgata tgtagacatt taggatattc tcctaaaagg gttttgcaaa
     5221 cttggactag gaatatgatt caaaatgatt tagaactagc tgagatatgc tctaagagtg
     5281 ttggaataat atcagaaaag actaatttaa aaaatcatcc gcttgtagat aatgcagagg
     5341 aagaagaaaa acagattaaa aaagaaaata gccaacaaga atatgatgat ttaattccta
     5401 atcaagacgg tggcatagat gaaacataat gattattgga gaaagagatt tgaacaatta
     5461 gaagaagctc aaaataacaa aagtgtaaaa tattatcttg aattagaaaa gcaatataaa
     5521 ctagctataa atagtataga aaaagatata ttagcatggt acaacagatt tgccaaaaat
     5581 gaaggaatat ctttattaga agctaggaaa ctactaaata caagagaact agaagagttt
     5641 aaatggagtg tagaagaata tattaaatat ggtaaagaaa atgctataaa tcaaaagtgg
     5701 atgaaagagt tagaaaatgc tagtgcaaga gttcatataa caaggcttga agctttaaag
     5761 ttacaaatac agcaacaagt ggaagtgctt tatggaaatg aacttgatgg tattgataaa
     5821 ttaatgagac atatttatac aagtggatat tatcatgcag cttttaatgt tcaacaagga
     5881 gtaaacgttg gttggagttt aatgaatctt gatactaata gaataaataa agttatctct
     5941 aaaccatgga ctagtgatgg attaaatttt agtgaaagga tttggggcaa acatagacct
     6001 gctttaatta atgagttaca tactaagcta actcaatcaa ttattagagg tgaaaatcca
     6061 aagaacctag taaatgactt ttctaagaga tttaatgtat ctaaatcaca agctaagaat
     6121 ttgataatga ctgaatcagc tttctttgca tcagcttcaa gaaaagattg tttcaatgat
     6181 ttagatgtag agaagtatga gattattgcc acactagact taaaaacatc aaatatatgt
     6241 agagagttag atggaaaagt atttgatatg aaggattatc aagttggagt tacagctcca
     6301 ccatttcatt gtcattgtag gacaacaaca gctccttggt ttgaagatga agaaggttac
     6361 agagcagcaa aaggagaaga tggaaaaaca tattatgtac catctaatat gaagtataat
     6421 gagtggtatg agaaacatat agagggtaga cttggaaaag aaaaagctga tactcttaga
     6481 aaaatgcaac taaatgagag taaagataga aaacaatttg aagaatataa aaaggtatta
     6541 ggaaatgaaa taccttctaa atttgatgaa tatcaacata tgaagtataa tgatactata
     6601 aggtatgagg aagctaaaaa gctttataag gatgtaaatt ggcaagttaa gaatcagaga
     6661 aatttaacta gtggaagtgt tcattcagtg ccatttgaat caaaaccaaa tagtgtattt
     6721 gataattaca aagatggtaa attaattcaa agaagatact atggtaatac aggaaaacct
     6781 agattagatt tagacttaac tgaccataga aacccaaagc aacataaaat tgtacctcat
     6841 aaacatgatt ggttagctga tgaaaataac catgataagg taaaaagaga gaaggatatg
     6901 gagcttacaa aagctcataa aatagcaaac aaagatattt tgaaaggaga gtaaaatgga
     6961 taataaattt aagaatttac aacatttaat tgattctatt gatttaggat tagatataga
     7021 atttgactta tataataaac catataatat atctattgga gatgatgata caaggtttat
     7081 tactctttgc cctaatggag atacaaagta ttataaaaat ggtaaggata tggttgataa
     7141 ttacaacatt gatggtaaat tactaaagga tttatggaaa gatatacaaa tagtaaatat
     7201 gtaaaagcac ttgctaagtt ataaattagt aggtgctttt attatgtaaa aatttattga
     7261 gagggtgatt tgaaatgctt aaattatata ttttatcaat aattgtgttt tgtacagggc
     7321 tttatttatt taaaatgaag attgatagta atgaggaatt gattgagatt cttaaaaata
     7381 aaaaagtgag aagaaagtgt aattttattt ttatggcttt atttccacta cttaatttta
     7441 ttttaggtgt gatatttatc atatcttctt tactagttag caatgaagat atgattaaaa
     7501 atttaaaggg
//
LOCUS       DAYAOY010000145         7262 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAYAOY010000145 DAYAOY010000000
VERSION     DAYAOY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.149"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            558..1049
                     /gene="infC"
                     /locus_tag="ACGI6B_003684"
     CDS             558..1049
                     /gene="infC"
                     /locus_tag="ACGI6B_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFG9753091.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            1077..1271
                     /gene="rpmI"
                     /locus_tag="ACGI6B_003685"
     CDS             1077..1271
                     /gene="rpmI"
                     /locus_tag="ACGI6B_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFG9753092.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1327..1683
                     /gene="rplT"
                     /locus_tag="ACGI6B_003686"
     CDS             1327..1683
                     /gene="rplT"
                     /locus_tag="ACGI6B_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFG9753093.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1993..2538
                     /locus_tag="ACGI6B_003687"
     CDS             1993..2538
                     /locus_tag="ACGI6B_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753094.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2525..2698
                     /locus_tag="ACGI6B_003688"
     CDS             2525..2698
                     /locus_tag="ACGI6B_003688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753095.1"
                     /translation="MKGNKSDLHGVEPIYTGNRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2973..4898
                     /locus_tag="ACGI6B_003689"
     CDS             2973..4898
                     /locus_tag="ACGI6B_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9753096.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5299..7203
                     /locus_tag="ACGI6B_003690"
     CDS             5299..7203
                     /locus_tag="ACGI6B_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9753097.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3055 a          719 c         1370 g         2118 t
ORIGIN      
        1 aatataatgt aggacttgtt atgtagtata gtaacatatt agaatataat gtaggatttg
       61 ttatgtagta taatagtata ttagagtaca atatagaatt tgttatgtag tatagtagta
      121 tattagaata caatatagaa tttgttgggt agtatagtgt tatattagaa tacaatatag
      181 gatttgttat gtagaatata tagagtcatg tagaatatac atatataaaa ggattttggg
      241 tataaattta aaaattaata gcaaaatgag tagtatagac atataattag tagtaggaag
      301 taattaaatt tttcctaaac aaaaaaaata gttgcaaata atttgattaa gtgatatagt
      361 aaataaggta agaataaaac aaagaagaag tccgcttctc accttacgcc ggaggttagt
      421 aaggtaaata taagttaaag cttaatttta atttaagttt gtttaaattt ggtaacttat
      481 attgcggatg attacgtcat ccgcttttta ttttataaaa attttggagg tgtcctacaa
      541 ttagcaaaga attagcaatc aatgaacaaa taaaagataa ggaaataaga gtcctttcac
      601 caacaggtga acaacttgga gtaatgccta ctaaagaagc tcaagcaatg gctaatagca
      661 aaaacttaga tttggttcaa atctcgccga atgcaaatcc accagtatgt aagataatgg
      721 attatggaaa gtttagatat gagcaagcga gaaaagagaa agaagctaag aaaaaacaaa
      781 aaactatagt tgtaaaagaa gtaagactta gaccaggtat tgaacaaaat gatttaaata
      841 cgaaagctaa taatgctata aagttcctta aaaaagggga taaagtcaaa gttgaactta
      901 gatttagagg aagagaatta ggacataaag acataggtaa agaagttatg cttaagtttt
      961 tagatataat aaaagagttt ggagaaccaa caaaagcccc tgcatttgag ggtaataata
     1021 tggttgtaat tatagatcca aaaaaataga tgattaactg agaggaggaa tttgtcatgc
     1081 ctaaaatgaa aactcataga ggtgcagcaa agagattaaa gaaaacagga actggaaagt
     1141 taaagagagc taaagctttt aaaaaacata tattaactaa aaaatctgca aaaactaaaa
     1201 tgaacttaag aaaatcaact ttagtaagtg atggagatgc taagagaata gcacaattat
     1261 taccatacta agatacagat tgtaaataat aactatagaa gagggatata aaaggaggtc
     1321 ataacgatgg caagagttaa aaaagctatg aacgctcgta aaaagcataa gaaaatatta
     1381 aaacttgcaa aaggattcag aggatctaga agcaaattat atagaccagc aaatacattc
     1441 gtaatgaaag cattaaaaaa tgcttatata ggaagaaagt taaagaagag agacttcaga
     1501 aaactttgga tacaaagaat aaatgcagct gctagaatga atggaatatc ttattcaaga
     1561 ttaatgaatg gattaaaatt atctggtgtt gaagttaaca gaaaaatgtt atctgaaatg
     1621 gctatacaag acccagaagg atttgcaaaa ttagcagaag ttgctaaagc aaaattagct
     1681 tagtattgaa tcatgagatg taaagtcttt gagttatata aataattcaa ggactttttt
     1741 attttttgaa aaatacttat tagtgtcatg tacaaccaat tttataatga taaataaaat
     1801 aagcaataca atagaatttt atgagattta ttataagaat tgttacaatt atttatgata
     1861 gctataaaaa gtcatagtaa aaatgatatt gatgtatgtt gatatatgta taaagtttcc
     1921 aatcaatctt gaattaatat tatcaacatg ataatattat cttattaata atataaataa
     1981 gggggacata atatgaatga aaaactaatg gattgttttg taaacccaat taaatgcaag
     2041 ttattgattg aaattttttc aaataatcga actacagcta aacaattagg agaaaaattt
     2101 caagatatat caaaggcaac actttatcgt taccttaata aaatgttgaa agatgatatt
     2161 ataaaagtgg ttgaagaaaa taaaataagg ggaactgttg aaaaagtata tgctatgaag
     2221 ttagatatag ctgatgatgt gaacaaaatt atagatgaga actctggaga agaatatgct
     2281 caactgttta ctcaatatat gatgggattt ttaagagaat tttatgaata cgctgaaaga
     2341 aaggaaaagg atatcctaaa agatggttca ggattttctg tctgtccagt atatgctagt
     2401 attgatgaga tagttgaagt gagtaaaaaa attgctagta tactagaacc tctaagaaat
     2461 aactcacatg aagaaggtag aagaatgcat tctataggag tgataattac tccacctaaa
     2521 aaggatgaag ggaaataaat ctgatttaca tggggtagag ccaatttata cagggaatcg
     2581 ttttatgatt tacatatgta tccagagcaa agtatctctg cttggattgt acttcattgt
     2641 accatttcat ccaatgactt ggagtggtac aatgaagttt tttaattttt catattaatg
     2701 ctaaagaaat gaactttaga ccacatatgc aattaggttg cattatacaa ttagaccaca
     2761 tatacaaaat aaaaatcagt atttttatag cagataaatt gactgtcatt cttcttaaaa
     2821 atcatctatt agttattgtt agcatattga aagaaattat agtaatctat tgaataaatg
     2881 aatattgcct ataatcatat aaactgctat agtttaaaat aaagcaggac gttaaatttt
     2941 atagtaatct aaaaatttaa gagggggatt ttatgaaaat aaaatttgtt attatgccat
     3001 tggtgatatt tttaatggta ttatttgtgg ggtgtcaaag tacaaaagat acagaagtta
     3061 aagcaaaaga tacagaaatt aaaatattag ggacaggtga tttacattca attttgacag
     3121 actctatggt ttcgtatgta aatgaggaaa gggaaaagaa taaaaatctg cttatggtag
     3181 atgcgggaga tttctatggt acacaaagta gagaaatgtg ggaatggtct tctggaaaaa
     3241 agttaattaa tattaaaaga aatggtagag cagagtatga tgacataatt aagagtagta
     3301 aagatgaagt acctatagtt aaagatatgg caaagttgaa atatgatgct gtagttttgg
     3361 gagataatga atttgtttca aatgataagc aaagtcttga taaactggtt tctgatttta
     3421 aaaataataa tatgccattg gtatcagcaa atatatatga acaatctgga gaaaattatg
     3481 tacaaccata tgtaatgaaa aatattaaga cagatgaggg caatgtaaaa gttggaatac
     3541 taggtcttac tataaaagaa gtaggggaaa cccttggtct tgaggatttt gaaaaggata
     3601 aaaaagctag agagcttgga gaacaagctg attacaaagg aaaattatat gcaaatgatt
     3661 tagtagaaga tgcaaaaaaa tggataaaag ttatggaaaa agaaagtcca gacataatag
     3721 ttgcagttgt acactcaggg gaagaaccta aaacagatag aaatccagga aatagaataa
     3781 aagagttagc aactacagtg gatggaatag atgcaattgt agctggtcat actcatgaaa
     3841 aaatagatga acacaaatat aaaaataaat ctggagatga agtaattgtt acacaaccag
     3901 gttggcatgg agatagagta tcagagatta atttcaaact aaataaagag aatggaaagt
     3961 gggacataaa ggaaaagtct agtaaattaa aaataattga taaagaagta agtatagttg
     4021 ctactgcaga tttacattca catttctctg ataaattaac agttgacctt gttaatgaac
     4081 gaagtaaccc aattgaacct gttgtagtag atgcaggcga cttcttagac cctcaaacag
     4141 atgaaatgac tcaatggtat aaagagtgga aaagtataag agataataat agtgataaaa
     4201 cgattagtag atgcccaatg gtcatggata tgaatcaggg aatgtatgat gcagtagtac
     4261 ttggaaatca tgaatttgta tctgaaaaag atgaattttg gtcagatgaa cgccttttag
     4321 atgctgttat agaggatttt gaacaggtgg caatttctgt gttatcggca aatatatata
     4381 ggcaatctgg taaaaattat gttaaaccat atattataaa agatgtggag acgagtgaag
     4441 gtaatgtaaa agtaggaata ttgggactta ctataaaaga agtaggtaaa ggtcttaaaa
     4501 atgtaaactt acaagagcaa tttcaatata aggataaatt gtatgcaaat gatttagtgg
     4561 aagatgcaaa gaaatgggta aaagttatga aaaagaaaaa accagatata atagttgcag
     4621 ttgtacattc aggagaagaa ccaaaaaatc caaaacatcc aggaaataga ataaaagaac
     4681 tagcaactac agtagatggt attgatgcaa ttgttgctag tcatactcat gaaaaaatag
     4741 atgagcatga atataaaaat aagtctggag aaaaagttat agtgacacaa ccaggtgagc
     4801 atggtaaata ttattctaaa attaccttta aagtaaataa agaaaagggt tcgtggacta
     4861 ttaatgataa attcagtaaa acaattgagg ttgaataact ttataaaatt tatagataaa
     4921 gtaaattatc tgatattggc atgatatata agataaagtt tctaatatta gtataatgca
     4981 taatgtgaaa tattttaata ataagagaat aaacaagaca aataaaaata ttttaacatg
     5041 taagatatag tatgaactat tatactaatg cttactttat aatttgaaca tgttagaata
     5101 agtcttattt gaaaatttta gtgtactaga gtaagtatta ttttactagt aaaaaatatt
     5161 gagaactata tgtagatttg cttaaaaagg aatttacata tagttctttt cgtataataa
     5221 atagtacaat cttatatggt aaagataaag tgtgatatta aattttatta tcatccaaaa
     5281 tctaaggagg agtctcttat gaaaataaaa tttattatag tagcatcaat aatattttta
     5341 atagtattac ttgtggggtg tcaaagtact aaagacacag aaatcaatgt actcgccaca
     5401 agtgatttac attcaattat tccagataat ttaatttcct atgtagaaga ggagagaaaa
     5461 tatgatgaga atctacttat ggtagatgca ggagattttt ttgacatgca aagtggtgag
     5521 atgaataagt ggtttactgg tcaaaaatta gtaagattta aagatgggat accagagtac
     5581 aaaaaaatag ctgaaccacg tgaaggagaa gttccattgg ctaaaaaaat ggcaaagctg
     5641 aagtatgatg cagttacatt tgggaatcat gaatttgtat ctaatgacaa gcagagcctt
     5701 gataaattgg tttctgattt taaaaataac aatataccat tagtatcagc aaacgtatat
     5761 gaacaatctg gagaaaacta cgtaaagcca tatgtaatga aaaatataaa tactgacaag
     5821 ggaactgtaa aggttggtat acttggtctg actataaaag aagtagggga acgttcaagg
     5881 tttaaaagtt ttgaacaaga taaaaaggct agagaacttg gggaacaacc tcaatacaaa
     5941 gggaaattat atgcaaatga tttagtagaa gatgcaaata agtgggtaaa tgttatggaa
     6001 aaaaaagaaa agccagatat aatagttgcc attgtacatt caggtgaaga gccaaaaaat
     6061 cctaaaaatc caggaaatag aataaaagaa ctagcaacaa ctgtagaggg aatagatgct
     6121 attgtagcag gtcatacaca tgaaaaaata gaacagcata catataaaaa taattctggt
     6181 gaagaagtaa tagtaacaca gcctggagct catggtaata gtatatcaaa gattaatttt
     6241 aagttggata agaagaatga taagtggttt ataaaagaca aatatagtga attaaaggtt
     6301 tttggaaagg aagtaaatat aattacaact tctggtctga atccaaaatt ttcagatgaa
     6361 atggcagtta atttgtttaa tgaaagagat agagatgttc aacctatctt tatagatact
     6421 ggagattttt taaatactaa tacaaaagaa atgactaagt gggctaaaga gtggcaatac
     6481 aatgaaaata aaggcatcta taaaaaaata aatgaatggc caattgtata tcttggttat
     6541 gatgctgtag tacttggtag ccatgaattt acagaggata aagagacttt tgaggaaaaa
     6601 aaatctacat tggattatat tatagaaaac tttgaagaaa tgaagatacc agtattatca
     6661 gctaatatat atgatgaatc tggagaaaat tatgtaaaac cttatataat tcataatgta
     6721 gaaactaggg aaggaaatat aaaggttgga gtattaggtc ttactactac tgataaggat
     6781 gaagaagact taagtaactc aagcttaagc aaagactcaa agacttcgaa aattcctgaa
     6841 gaaacaggaa agttatatac taataatcta gtaaaagatg caagcgaatg ggtaaaagtt
     6901 atgcaaaagg aaagtcctga tgtgattgtt gcagttgtac attcagataa taatagtact
     6961 ctaaaaaatc caagtagtga agtcaaaaaa cttgcaatgt cagtagatgg aatagatgct
     7021 attgttgtag gtcatacgaa aaataaggta gaagaacata tttacaaaaa taagtctggg
     7081 gatgaggtta tagtaacaca atctggagaa gattgttatt caaaaataag tcttgaacta
     7141 agaaaagaaa atggcaagtg gaatatcata aacaaatata gtaaagcaat aaaaatgaag
     7201 tgaatttatt gatatataaa ccattattaa cactgatttt tataatataa taaatactga
     7261 tt
//
LOCUS       DAYAOY010000146         7228 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYAOY010000146 DAYAOY010000000
VERSION     DAYAOY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.150"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            142..1578
                     /locus_tag="ACGI6B_003691"
     CDS             142..1578
                     /locus_tag="ACGI6B_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HFG9753098.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            1626..2453
                     /locus_tag="ACGI6B_003692"
     CDS             1626..2453
                     /locus_tag="ACGI6B_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9753099.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    2749..3011
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3087..5753
                     /locus_tag="ACGI6B_003693"
     CDS             3087..5753
                     /locus_tag="ACGI6B_003693"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFG9753100.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            6178..6888
                     /gene="rgaR"
                     /locus_tag="ACGI6B_003694"
     CDS             6178..6888
                     /gene="rgaR"
                     /locus_tag="ACGI6B_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HFG9753101.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         2959 a          703 c         1331 g         2235 t
ORIGIN      
        1 aatgaaaata atgattgaaa tagagttgtt gagaaaacta aataaatcat attaataata
       61 gtagttaaat aataaataaa aaatagtatc tttgtaaata ttaaacatat ataagtttaa
      121 tttatggagg tactattttt tatgaatact agaaatgaca attctaaaat actagaaata
      181 tataaaatga agctttttgg cgaacttgta aaagcagtgt ggaatgatga catagaggat
      241 attgaaaact tacctaataa aattttaaat gaaaatttaa aggataattt aaataattat
      301 tttagtggaa aatttgataa agatactatg gtaaatttaa ttagggtaac tatgggattg
      361 gatgtgacag aagatacaga taaaaattta aaagagtcac tacatgaagc tgtaaattta
      421 gatgaagtca aaagaccaat aatatctgta atatcagatg cttgcaaata ttgtgatgaa
      481 aataataagg aagatgagtc atgtaagata agaagtaagc atattaattg taatgaggaa
      541 gatgtatgta gtgcttgtgg tgattgtgta tcaagatgta agttgggtgc aatttcagat
      601 aaaatagaat ttataccaat gataaaattg ttaaaagata taaagagccc tgtatatgca
      661 atagttgcac cagcttttgt aggacagttt gggaaagatg taagccctgg taaattaaga
      721 actgcattaa aaaagatagg atttgaagat atgatagaag ttgctttggc agcagatatg
      781 cttactgcaa aagaagcata tgattattat gagcatatga aaaaggacga agaaggatgc
      841 tttataacta gttgttgctg tcctatttgg gttagtttga tacaaaatag ttttcctcaa
      901 atattggaga atgtatcacc atccgtttct cctatgattg cttgtgggag agctgtaaaa
      961 attttaaatc cagatgctaa agttgtattc ataggtcctt gtatggcaaa aaagaaagag
     1021 gcaatgttag aagatataaa agatgcagtt gattttgtgc ttacatttaa agagttgcaa
     1081 gaaatatttg atgcattaga gttaaatcca gcaataatgg atgagagtaa tagagtagag
     1141 gcgtcccttt caggtagagt ttatgctaga acaggaggtg taagtaaagc agttaagtta
     1201 tcagttagaa gaattgacaa aaatataaaa tttgaagaaa aggcatttga aggaacaaaa
     1261 gagtgcatgg acggtctaaa gaaagttcta aataaagaaa tagaagctac atttatagaa
     1321 ggaatggggt gtgtaggagg ctgtgtagga ggtccgaagc gtattttaag tgtggaagaa
     1381 ggtactaaga atgttgatga atatagtgaa agtacgaata tggaaactcc atttgataat
     1441 ttaaatgtat tacaattttt aacttctttg ggaatcaaaa gagtagaatc tttaggagaa
     1501 aaagaagaag aaaaggttaa aaaaatattt agtagagata taaaaagtaa taagaaagag
     1561 aataaatata aaaaataact ttttaagtct taaacttatg cttttataaa agggagatat
     1621 gggggatgaa aattaaagat aataaggctg tttttcatgt atttataagg atatttctta
     1681 tagctgtttt aattttaact ggaacattat tggaggaaaa aattaataat gttccagttg
     1741 aaaatataag ttcaaaaggt gaaaatacac tggaactgct ttctcaaagt aatactttag
     1801 gagaaatcat atacaaagca tctcaagaag aaacaaaagg tccaggggct acttctgaaa
     1861 aagtagcata cataacaata gatgatggtc catcaaaata tacaaatgca ctacttgata
     1921 ttttaaaaga aaatgatgtc aaagcgacat ttttcatgct taatcagaat atgaaaaacc
     1981 acaaggaaga agtaaggaga gtcttggaag aagaaaactc aataggtttt catagtgtaa
     2041 gtcatgatat acataagtta tataaaagcc cagaagtaac agttggggaa tttaatacat
     2101 gcaataatac tctacaagaa ataactggtc agagttcaaa attaataaga ttaccatacg
     2161 gaagtaaacc atatatgcca gaaggttcat ataataaact tatggcaaat gattatttaa
     2221 tttgggactg gaatttggat acacaggatt ggaagtcttc tacaagtcag atagtaagta
     2281 atatattata ttatggaaga aaaagagatg aattagtagt attaattcat gagaaagagc
     2341 aaagtttaaa tgctttaaat aatattataa gaatcttaaa agagagagga tatacaatat
     2401 tgccaattac agaaaatata acacctaaaa atttctggca agaaaactta taataaattt
     2461 aaatatagta aaagataaag taaattattt ataaaataaa acatataata taaaaaatac
     2521 gaataagagg tatactgtaa ggataatata aaattaatga gaaagcatta aaattaaaat
     2581 aaaataatct tgcaattatt gacattataa gacagaataa ctgtaaaaac atattttaaa
     2641 atataacaac tatataaaag agaatagaca taattaagaa aattcagaaa aaataaaata
     2701 aaaataattg acaaagagaa taatatttgt atataataat atgtaatatt aaagactgag
     2761 aagaagagga gtaagtaaat actactaaaa gagaggaaaa ttcactggct gtaagatttt
     2821 ctaagtataa gatttactga atgtagcttt ggagtttcta aactgaacta taagtaagtt
     2881 tagacggtta gaaccgttaa attctttgag agtcaaagtt gataaaataa cattaaccaa
     2941 taatttaatg atatgttaat gtattttaag ctttggaatt aaggtggtaa cgcgagcttt
     3001 tcgtcctttt taaagaggat gaagagctct tttttatttc taaagatatt taaaaaattt
     3061 atttataaaa tacaggaggc aggacaatgg aaaatacgaa tttatcaaaa acttataacc
     3121 caaaagattt tgaggctagg ctatataaaa aatggatgga tgaagggtat tttaaatcta
     3181 aaccaaatcc agataaaaaa ccatttacaa taatgatgcc accaccaaat ataacagggc
     3241 aattgcatat gggacacgct cttgaccata cacttcaaga tatattaatc agatggaaac
     3301 gtatggatgg atatgaagct ttttggcttc caggaactga ccatgcttct atagcaacag
     3361 aagttaaagt tgttgaaaga ataaaaaaac aagaaggcaa gacaaagtat gaaataggta
     3421 gagaagaatt tttaaagaga gcctgggaat ggaaagatga atttggagga aaaatatcta
     3481 atcagcttaa acaactaggt gattcttgtg attgggataa agaaagattt actatggatg
     3541 aagggtgtaa tgaagcagtt atagaatttt ttgtaagcct atatgaaaaa ggtcacatat
     3601 acagaggaaa tagaataata aactggtgcc cagattgcaa aactacttta tcagatgcag
     3661 aagttgaaca tgaagaacac gacggaaact tttatcatat aaagtaccca ctaaaagata
     3721 gtgaagactt tttagaaata gctacaacta gacctgagac aatgatagga gatactggta
     3781 tagcagtaaa tccagaagat gatagatata aacatttaat aggaaaaaca gcaatacttc
     3841 cattagtagg aagagaactt ccaatagttg cagatagtta tgttgattta gagtttggaa
     3901 ctggtgcagt taagatgact ccagcacatg accctaatga ttttgaagta ggattaagac
     3961 ataatcttga gcagttaaat actatgaatg aagatggaac tatgaatgaa gtttgtggaa
     4021 aatatgaagg tatggataga tttgagtgta gaaaagctat cgtagcagac ttaaaagaac
     4081 aaggttacct aattaagata aaagagcata atcataatgt aggaacttgt tatagatgcc
     4141 atacagtagt tgagcctaga ttatcagagc aatggtttgt taagatggaa gaacttgcaa
     4201 aaccagctat agatattctt aaaaaaggag aattagaatt tgttccagat aaatttgata
     4261 aaacttattt acaatggcta gaaaatataa gagattggtg tatatcaaga caattatggt
     4321 ggggtcatca aataccagct tattattgtc aagagtgtgg agaaatagta gttgctagaa
     4381 aaatgccaga taagtgtcct aaatgtggaa gcactcattt taaacaagat gaagatgcct
     4441 tagatacttg gttcagttct gcattatggc ctttctctac tctaggatgg ccaaataaga
     4501 ctgaagcttt agattattat tatccaacaa gtgtacttgt tacaggatac gatataatat
     4561 tcttctgggt agttagaatg gcatttgcag gtatgttctg tatgaatgaa aagccatttg
     4621 accatgtttt ggttcatgga ttagtaagag attctcaagg tagaaaaatg agtaaatctt
     4681 taggaaatgg tatcgaccca ttggagataa tcgagcaata tggagctgat gccctaagat
     4741 ttacattaac aactggaaat tccccaggaa atgatatgag attctatatg gaaagagtag
     4801 aatttgctag aaactttgca aataagttgt ggaatgcatc aagatttgta tttatgaata
     4861 ttgatgaaga tataattaaa aacatgacta gagaaagtgt taaagaggac ttaactctag
     4921 ctgataaatg gataatatca agagcaaata atattgtaaa agaagcaact aataacatgg
     4981 ataagtttga tttaggaata gcacttcaaa agatatacga ttttacttgg tcagaatact
     5041 gtgactggta tattgaaatg gttaaaccta gattatatgg agaagatgca aatgctaaat
     5101 ctgcagcatt atatacatta acttatgttc ttgaaaaaat attaaaatta ttacatccat
     5161 acatgccatt tataacagag gaaatatata ctcatcttcc tactgttgaa ggatgtataa
     5221 tagtatctga atggccaaaa tataatgaag aagataacat ggctgaagaa gaagatatga
     5281 tgaacttatt gatggaaggt ataagaagta taagaaatgt gagagcagaa atgaatgtac
     5341 caccttctaa gaaagctaaa ttgataataa tcccaagtga agaaaaaata gaagctattg
     5401 agcttggtaa agactatttt ataactttag catctgcttc aaatgtagag atagcaaaag
     5461 ataaatctaa tgttccagaa gatgcagttg gagtagttat tgatggtgtt gaaatattta
     5521 tacctcttaa tgaattagtt gattttgaaa aagaaattga gagattatct aaagaaaaga
     5581 aaaagttaga aggcgaaata aagagagtta atggaaagct tgcaaatcaa ggtttcttag
     5641 caaaagctcc tgaaagttta attgaagaag aaaaagcaaa aaaagaaaaa tttgaagaga
     5701 tgattaaatc tgttgaggaa agattaacaa atttagaatc taaaataaaa taaatataag
     5761 aattagtaaa aacatacaaa aagtatatat tctatacttt ttgtatgttt ttctttatat
     5821 atatacaaat atgatgttat aattctttac agatgtgtta tatttttatt ttatgttgtg
     5881 aatatatgaa ttttatgtct taaaatttat aagcaataaa aatttgatat attatgatgt
     5941 ttaatatagg gtaataaata catattatat ttattttact atattttttt acataatata
     6001 tctagaaaag tataggaatt tatcgtaatt taagtttaaa tattaaagag aatttctata
     6061 agtagtttaa tcgacaattt aaatttataa agaaatttaa atttgaaagc tagtgagtac
     6121 agtttggaaa ttgaaataaa gtaaactaaa tgatatttta gatttggagg tgatattatg
     6181 ttcagggtag ttatatgtga tgacgaaaaa atacaaagaa gcatactaaa agagtttaca
     6241 gaaaaacttt tgtctgaaag atgtctaaat tatcaaatac tggaattttc ctgtggagaa
     6301 gatttaatat ccaaataccc agaaaaaatt gatattatat ttttagatat ccaaatgaaa
     6361 gatatcaatg gaattgaggt ggctaaaaaa ataagaaagt ttgatgaaaa agtagaaatt
     6421 atatttacaa cggctttttc agagtatgcc ccccaaggtt atgaggttcg tgcttataga
     6481 tatttagtaa agcctataga gtatagtaac tttgcaatgg gtgtaaatct ttgtattgat
     6541 aaccttcaaa gaaagaagga gcgttatata gttttaaata gtaaaaaagg atttaataga
     6601 atactgatag attctatttt atatgttgaa actgtcaaga gagatttagt cgtacataca
     6661 ataggtagag attataaagc taatatgagc atgaaacaag cagaaaaatt actgtgtgaa
     6721 aatggctttt ttagatgtca tacaagtttt attgttaatt taagaagaat agaagatctt
     6781 agggataata tgattactat taatgaacaa tttataccta taagtaagta tagacttaaa
     6841 gatttaaaag tagcattaac aagcttgtta tgtgatataa tgtattaatt ttaaaaaggt
     6901 aattgaaaat atcgtaaaaa agatttaatg tcatcacagg gctattgatt gaaactgtct
     6961 taatatataa aatgttattt atattaagac agttttatta tgcaaaatag cagtttatag
     7021 atttttaata tggtttaaca tgtatatgta gaggttccat tatagtagca tgattattaa
     7081 ttagtagctt gattatcaat atactattat tgatttttat atgcattata tgtattttta
     7141 gaatatgatt acctttttaa aataatgatt atttataaaa ctctatagat atttgtttca
     7201 tttagctacg catactttaa ttaattat
//
LOCUS       DAYAOY010000147         7170 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYAOY010000147 DAYAOY010000000
VERSION     DAYAOY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.151"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(248..1087)
                     /locus_tag="ACGI6B_003695"
     CDS             complement(248..1087)
                     /locus_tag="ACGI6B_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9753102.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1111..1869)
                     /locus_tag="ACGI6B_003696"
     CDS             complement(1111..1869)
                     /locus_tag="ACGI6B_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9753103.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1916..2389)
                     /locus_tag="ACGI6B_003697"
     CDS             complement(1916..2389)
                     /locus_tag="ACGI6B_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9753104.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2418..2834)
                     /locus_tag="ACGI6B_003698"
     CDS             complement(2418..2834)
                     /locus_tag="ACGI6B_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9753105.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2881..3933)
                     /locus_tag="ACGI6B_003699"
     CDS             complement(2881..3933)
                     /locus_tag="ACGI6B_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9753106.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4329..5009
                     /locus_tag="ACGI6B_003700"
     CDS             4329..5009
                     /locus_tag="ACGI6B_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFG9753107.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5142..5462)
                     /gene="sugE"
                     /locus_tag="ACGI6B_003701"
     CDS             complement(5142..5462)
                     /gene="sugE"
                     /locus_tag="ACGI6B_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFG9753108.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     regulatory      complement(5532..5638)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            5844..6671
                     /locus_tag="ACGI6B_003702"
     CDS             5844..6671
                     /locus_tag="ACGI6B_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFG9753109.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2435 a         1298 c          842 g         2595 t
ORIGIN      
        1 aataaattta tatttttttg aaattactta atattttata taaaaatgaa aaagcacctc
       61 attctgatta aaaatctttc tgagatactt tttaaaatat taaatactac atatagctta
      121 cataagttta aaatttaatc tattataaaa ttaccctaat tgccaaactt tttaatataa
      181 attttccaac atcctacaaa tataatcaat agatatagaa tcctaactta caccaatata
      241 tatttattta aaatagtcct aaaatacctg taatagctcc cactactatc agtataaaca
      301 ttatttttat tggagaaaga ccttttttta atgcaaagag tactaataaa gtaagtacca
      361 aaggaattaa tcctggaaat atcttatcaa gtaaatccgt ttgaagacta aatttaaaat
      421 caccactagt ataacttatt ggtgtagata aattgataaa cctacctaca agccccccca
      481 tgactaaggt acctagtata gaagcccctt ctataacctt atttactatt ccaccttgaa
      541 gtatactttc aacagctttt ttcccatact tatatccatt tgtccacatt ccaaaagaaa
      601 ttgaagtcat tattatatat tgagctatta caaataatat aggtcctgca attgatgctc
      661 ctccctcagt taaaccaata caaacagcta acactatagg agttataact ccttgagtta
      721 aagtatctcc aattcccgct agtggaccca tcaagcctgt tttgatagca ttcataccat
      781 ctccacttat ttcatctgct ccattagctt tttcttcttc catagctatt gttattccat
      841 gtataacaca tccacatata ggttctgtgt taaaaaatgc tagatgagtc tccacttctt
      901 gttttaactc ttccttatct ggatataatt ttttaagtac tggcaacatt gaatgtgaaa
      961 aagctgttga ttgcaatctt tcataattgt aattagattg tgcaaagaaa aaccatctta
     1021 accatgattt tattacatct ttcttgctta acgttttctt agatacttca ttttttatat
     1081 ctgacataat attttccccc tcacatgtat ttaagatgta gcactatctt ttccgtactt
     1141 aattgaagta attgtaagag caagtattac tccaataaca gatattacta ttatatctac
     1201 tttaaaataa acagccatta gtattcctaa tatgaaaaag ggcatagtaa acttatttcc
     1261 aatcaatttt aaattcatag caatcccaag tgctggcaac atagccccaa ttacattaaa
     1321 tacatgtaac acattatcat tcattacttc caacatacca tctatcgcat ttggaccaaa
     1381 ataaacaact aataatgctg gtataaaact cattgcaaat aacagtatct gtggtggtac
     1441 tatatttata aatgctactc tatcaacatt ccctttttga acctctctat cagcccaatg
     1501 tgcaaaaaaa gaatttactg tcatctttcc aatccaaagt acagttgcaa cagttcccaa
     1561 tggtacagct attgccattg cagtacttac atctagctta gatgctatag ctaaagcagt
     1621 acctacatat cctgcaaaag ctgggtcccc catctgagct ccccctgctg atataaaacc
     1681 tagatatatc atatttatac ctgctcctat aattgctcct tgaacagggt ctcctaatat
     1741 aattcccgtt acaaatccag ccaatagagg tctattccat gtaaaaaatc caacataagt
     1801 caaccacgga ctccaagaaa gataataaaa aattgctatt aatactgctt gaattagtga
     1861 aatgtgcata ttcttcctcc ctattttctt ttaaagattt acaagatagt cttctttaca
     1921 ataatgttcc tacatcaatt ttctttgcat ctggtactat ttgtataaag catttaacat
     1981 cttttgatat tagctctcta aatatttctt tttcttcatc tgacacagat atatttttat
     2041 aaaaactttt tctaccaagc cctgctccca tacctccaac attcaattcc tttaaatcta
     2101 ccccactatt tactaaacct aagacagttt tagggtattt gaccaatact attaaatttt
     2161 catctccatt atgtgatttt aagacttcag cacctgattc tacatcatgt acttcaacct
     2221 taacagttga tggagctgcc atttgtaaaa cttgaactat aaatgggtcc tttactacta
     2281 aatcatccac gattactatc ctttttgctg atgtaatctt acaccaagca gtaataactt
     2341 gcccatgtat taatctatca tcaattcttg ttaatgctat attactcata attattcctc
     2401 cagattttta atttatttta caactttaaa acagttttaa catctttgat tcctgatatc
     2461 ccaacttcct cacataaatt cttcaagtct tttaacttca tagtatctct gtttaaaaat
     2521 aactctaaaa gcataggtaa atttacacct gtcaaacatt ctaactcaca taccttagca
     2581 aatttattta ttacaatagc agatgtatta aatggagttc cgccataaaa atcaagcaat
     2641 actaaaaccc cttcatcctt atatttctca attccatctt ctaactttct actaaactca
     2701 tctatgctgt caccatggtt taatcctaaa gtttctacat tttcctgttt gcctactatt
     2761 aattcagcac tttctaacat tgctttagcc atatttccat gtgtggctat aattattcct
     2821 gccatatgaa tcataccttt cttaaccttt tttatatgga ttattattta gtactctaat
     2881 ctaattatct atattaatca ttatcttacc aaagaaaata tcccttttgt atactttttc
     2941 aaaataagct ggtacttctt ctaaatttat tctatgtgtt actaattttt caacattcat
     3001 atcacccttt tgcatatgat acaatccagc actccattct tttcctggga atggaaagga
     3061 gtttccaaac caagttccct ttacagttaa ttcacttctt actatctttt caaattgctc
     3121 tctagtcaaa gctacatccc catatggtac acctgcatac aatactgtac ctcccttttt
     3181 agctagcaac agtacttgac cacatgtcag tggagtacca gccgattcaa taacaatatc
     3241 tgcaccatct ccatcagtta acctttttat ttcttctaca atattctttt cttttgcatt
     3301 tatacaaata tctgccccta actcttttgc caagtctaac ttctcatcaa atacatccac
     3361 agctattatt tttgtagaac caaatatttt tgcccattgt attgaaaata atcctattgg
     3421 acctgtacca agtactacaa cagtatctcc aactttagct tctgacctaa aaagaccatg
     3481 tcctgctata catactggtt ctagcgcagc ggcagtctca taacttatct catctggtat
     3541 ttttattaaa tttctctctt tcacctttgt atactctgca aaacaacctc caagttcttt
     3601 atttcctatt attgctacat tattacatct tgaatacaag ccttttttac attcatcaca
     3661 ttcaaaacat ggcatagctg gacaaacagc aactctatct cctattttaa aactccttac
     3721 ttctttacca acctgtgcca cttctccaga aaattcatgc cctaaaattt ctccaaccat
     3781 atgtggacct gtctttgaat attttgatat atcagaacca catattccag ctacttttac
     3841 tttaattatt acatcatctt tttctaatat ttttggtaca tccacatcct ccactcttgt
     3901 gtctcttata ccatagaatc taactgactt cactattaac cacccctact ttttatttga
     3961 gaatatatcc ctattgttta tatgttaaat atctaaagca actttcatgc caatatatgt
     4021 aagcattgta ttcatgcatt ttataaaatt atcattaaat gtgtgtcaaa gtgtgtcaaa
     4081 agtttcttat attaaaactt atgtgtgtca atataaacga cactaacatt ttatattagt
     4141 tctcttatta taagttttat aaaatgtact ttttttgtta tttttataaa attgttcgga
     4201 aatttccctt ctatttgtga tataatatta aatataaggt atcaatataa attttcatgc
     4261 gaatatcaga acttttttga cactaatgcc ttattcaata taataattta actttttgga
     4321 ggttgtttat gaaaaaagtg tatcaaggaa agactaaaga tgtttttgaa ttagataatg
     4381 gaaattactt acttaaattt aaagacgatg taactggtaa agatggagta tttgacccag
     4441 gcgaaaatag tgtaggtcta agtatagatg gaattggtag agctaattta gagacatctg
     4501 ttaagttctt tgaaattcta aacaatgctg gaattaaaac tcattatgta agtgctaatt
     4561 tagaagaagc aactatggaa gttctaccag caaaagtttt tggtaatgga ttagaagtta
     4621 tttgtcgtta tagagctgta ggtagtttct tacgtcgtta tggttcatgt gttgaagaag
     4681 gtgctaaact agattgttac gtagaagcaa ctttaaaaga tgatgaccgt tgtgaccctc
     4741 ttataacatc tgaaggttta gaagctttaa acattatgac tcaagctcaa tttgattcaa
     4801 tgaaaagcat gactcaaaaa atttctaaca tagttcgtga tactatagct gaaaaaggtc
     4861 ttgagcttta tgatattaaa tttgaatttg gattctacaa tgatgaagta atcttaatag
     4921 atgaaatagc atctggaaat atgcgtgttt ataaagatgg tgtaatagtt gaccctatgg
     4981 atttaactgc tcttttatta gataaatagt atatacatat aatttacaaa tataataaac
     5041 ttataaataa gataagctat gaagtaaaga ctttttaaat gttgattatt tttaaacatt
     5101 ttctcgcctt taatttcata gcttatttaa attaatatac tctaattagt agataatagc
     5161 tttaacccta caattcccac cacaataaaa cctatacatg caattcttga tatactaata
     5221 ggctcattta aaatagccat gcctacaata gctgtaccga cggtaccaat tccagtccat
     5281 attgcatatg aagttccaag tggaagatgt tttagtgcta atgacaaaaa ataaaaactt
     5341 acaatcattc caacaacagt aagtatactt ggagttaatt tagaaaatcc atgtgaattt
     5401 tttagttgaa aagcccaaaa cacttcaaac aaacctgcaa caaacaaaaa taaccatttc
     5461 ataaattcat ccctcttttc aaatatgtct cttatttttc caataaaata acctgagaaa
     5521 tattataaaa tatctcccag gtttttgtcc ttccgtgttc acaacaaaaa ctatgttgtg
     5581 ggttttatct cggaccagac caatcattaa taattgcgga accctataaa actctattaa
     5641 ttattctatt ttatctataa taatactatc aacatatata aaaaaagtca atatatgggt
     5701 tccatatata ataaaaataa cttttatata ccttttaata ttcttattca cttctctcca
     5761 tacccatttg ggtcaatcta agaatatcta atttttattt aatatgttat aataacataa
     5821 aaatattgaa aggagtaatt tctatgaatt tctataaaat tgcagtatgt cagatgatta
     5881 ctacagaaaa taaaattgaa aatatcaatc atgctgtaga tatggtaaca gaagctgcca
     5941 tcaatggagc taaaattgta gttttgccag aaatgtttaa ctgcccatat gaaaataaat
     6001 attttcctaa atttgctgaa gaatatccag gtgaaacaac aacgatatta agtaaattag
     6061 ctgagaaaca tggaatttat ctagtttctg gtagtattcc tgagttagaa gatggaaaaa
     6121 tctataatac ttgctatgta tttgataaaa atggagctct aattggaaaa catcgtaaaa
     6181 tgcacttatt tgacattgaa gttacaggaa aagttagttt taaagaatca gatactctta
     6241 cagctggaaa tgatgtcact gtaatagata ccgaatatgg aaaagttggt atagctattt
     6301 gctatgatat tcgtttccct gagttatcac gtttaatggc tcttaaaggt gctgaaatcg
     6361 ttatcttacc tgctgcattt aatatgacta ctggtcccgc ccattgggag ttatctataa
     6421 gaatgcgtgc tttagataat cagatattct atgttggtgc tgcacctgct agaaatatga
     6481 atgcttctta tatagctttt ggaaattcaa gaatttcaga cccttgggga agaataattg
     6541 ctcaagctga tgaaaaagaa tgtattattt atgctgacat tgatagagat ttaattcctg
     6601 acataagaca acagttacca cttcttaagc acagaagaac tgatttatat gaacttaata
     6661 ctttgaaata aaacagaaca taaccccata aatcataaat taagtgttaa aaaaatttat
     6721 ggggttctca ttaatttgat ttaaaattaa tttcataaaa ataatgctaa tattaattta
     6781 attaaataat actaataatt actaatgatt atgctaatta tcagcattta ctaatttaat
     6841 cactattagt tatttctttt attaaatatc tttacttttt tacttattat gtcttctagc
     6901 tttacatttg gattatggta tttctttcta tttttagtca catctttcta ttgaattact
     6961 tgcttaaaac accaatgcac acatgccaga taatgttcac aattatttag aaatcttgct
     7021 ctaccttctt caatgttgat attatctctc ctagttgata ttatctttcc taaaatttct
     7081 tttaattaaa aaacaacatt agttgatata tcaagtatat tttaagcaac tgaaaaaaac
     7141 aataataatt gatatatcaa ctaattaatg
//
LOCUS       DAYAOY010000148         7170 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYAOY010000148 DAYAOY010000000
VERSION     DAYAOY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.152"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..420)
                     /locus_tag="ACGI6B_003703"
     CDS             complement(<1..420)
                     /locus_tag="ACGI6B_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9753110.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKY"
     gene            633..1106
                     /locus_tag="ACGI6B_003704"
     CDS             633..1106
                     /locus_tag="ACGI6B_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9753111.1"
                     /translation="MNGVEKYMVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNK
                     EALLKDLLKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRY
                     VFSLLAPHKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            1109..1417
                     /locus_tag="ACGI6B_003705"
     CDS             1109..1417
                     /locus_tag="ACGI6B_003705"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9753112.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            1531..2637
                     /locus_tag="ACGI6B_003706"
     CDS             1531..2637
                     /locus_tag="ACGI6B_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9753113.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2712..5390
                     /gene="mngB"
                     /locus_tag="ACGI6B_003707"
     CDS             2712..5390
                     /gene="mngB"
                     /locus_tag="ACGI6B_003707"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFG9753114.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5707..>7170)
                     /locus_tag="ACGI6B_003708"
     CDS             complement(5707..>7170)
                     /locus_tag="ACGI6B_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9753115.1"
                     /translation="NVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT         2790 a          940 c         1056 g         2384 t
ORIGIN      
        1 atactttgac aaaataggct ttattctttt tatgtcattt agtattgtaa atttactcac
       61 atacaagcta ttagctaaat cttctagttt tatatatgta ttgttataga gaatatattt
      121 taaaatatat tgatttcttt ctataggaga atctggtatt tcacttgaat ccataatatc
      181 atcagatatt aatgctagat actgttggta taatgctaaa ttaagaatat ctatataata
      241 tccttctcct ttttttattt taataatagc accatttttc tctaaagttc catttataga
      301 agtaatatca tttcttatag ttctctcact cacttttaaa tgattactta aagttttaca
      361 agtaatttta tcatcacttt catttaataa tttaaacaac gtgagcaatc tcttgtgcat
      421 aaatatcccc ccttatagaa tttcttctaa attatactat ttacactcat ataatacaat
      481 tactctaata aaattgtatt ataataattt atttccactt ctatatggaa attagttatt
      541 atcataatat aaatttccat gtcgaagtgg aaattagtta tatttaattt aattaggata
      601 atgattacaa tataaatata agttaagaca ttatgaatgg agtggaaaaa tatatggtac
      661 tacaagaaaa atatatattt atagataaag acttaaaaag caaagaagaa gtattaaatt
      721 ttattgcaga tagtgcttat atactaggta ttacaactaa taaagaggct cttttaaaag
      781 accttctaaa aagagaaaat gaatttccaa caggaataaa agatggattt gcgatacctc
      841 atacaaagag tgtaaatgta aaggaagctt ccatactcta tattaaaaca actaacgagt
      901 tagaatggaa aactttagat gatagtaagg ttagatatgt atttagtttg ttagccccac
      961 ataaaaatga aggaaatata catttgaaaa ctctatctaa attagccaca tgtttaatgg
     1021 aggatgattt tacaagtgaa gttaaaaatg ctagtgatgc aagtcattta gtaaaatata
     1081 taactaataa aatgaaggag gaataattat gaaaatagta gctataacat catgtatagc
     1141 aggattagca cacacaccta tggcagcaaa agctttagaa caagctgcat caaaaatggg
     1201 tcatgaaatc aaagtagaac aacaaggtgc tatgggacga gtaaatacta taacaaaaga
     1261 agaagcacaa gatgcggact ttatacttat agcatctgac caaactatat ctggaatgga
     1321 aagatttgat ggtaaaaaag tgataaaagt caaaataggt atagcgttaa aaaaaccaga
     1381 agctgtctta acaaagtgta tagaagctat ttcttaataa aaaaatctca ctctaatata
     1441 gaatgagaaa aatcaaaaat taaaaaattg tctaatgtaa ttataacata ttatcaaaaa
     1501 atataacaaa cttatatagg aggattaaaa atgagtaaaa aaaataaatc tcttttaagt
     1561 gaacttaagg gacacttaat gacaggtatc tcatatatgt tgccacttgt aataggctca
     1621 tctttagttg tagctatccc aaagcttaca gctttagcag tgggtataac aagtttagat
     1681 gcttataaaa atgtatctgg tttttatcat attctttact taatggaaca agttggttgg
     1741 actggcatag gtctattaaa tacggtatta gcaggattta tagcattttc aataggtgag
     1801 aaaccagcta tgggtgcagg ttttattggt gggctcatag caagtaatac taatgcagga
     1861 ttcttaggag cagtaatagc aggtttcttt gctgggtatg tttgtaagtt tttaaagaaa
     1921 aaaattaaaa tatctgggtc agcatcagga atgatgccac ttataataat gccacttata
     1981 acagttggat taactggttt tttaatgagt gttgtattag caggaccttt aggaaacata
     2041 aatacttcat taaatgcttg ggtagctgaa atgtgtcaaa atggaacaaa ttcggtggta
     2101 ttggctttaa ttttaggcgc aatgatagga ttcgatttag gtggaccagt aaataaagca
     2161 gcttggatgg caggaaatgc attattgtta gagggaatct atctaccagc gatattggta
     2221 aactgcgcaa tagttatacc tccactagga tatggtttag ctacattcat aagaaaatca
     2281 aaattttcca atgcattagc tgaaacagga aaaggcaata tagttatggg tataattgga
     2341 ataacagaag gtgcaattcc tttcacttta acaaatccat taaaattagt tcctgttaat
     2401 atgataggat gcgctttggg tgtaggatta tcagctttgt taggtgtaca tgctataatg
     2461 cctccagttg gtggattata tggatttata tcaataggtt ctggatgggc ttacttagta
     2521 ggtgcaatat ttggagcttt agtaatagca atattatcaa caactttagt agacttcaat
     2581 gaagatgaaa ctacggaaaa tacagaattt gatgaaatag agttagatat aatgtaaatt
     2641 aaaaacttaa ttttagataa ttgagaattg agtgtactct caattctcaa ttaaattttg
     2701 ggaggatttt tatgaaaaag acaaaagtac atgtagttcc acacagtcat tgggatagag
     2761 aatggtattt tacaacatca aggtcaaaaa tatatttaat gaaagatttt caagatatat
     2821 tagacatatt ggaagaaaaa gaagacttta aatactttac tatggatgca caagcttctc
     2881 tcttagatga ttatttaaaa tggagacctc aggataaata tagaataaga gatttagttc
     2941 aaagaagaag attagttatt ggaccttggt atactcaaac agaccaattg gttatatcag
     3001 gagaatctat aattagaaac ctttactatg gaatagatag atgcaatgaa tttggtgaac
     3061 ctatgagggt tggatatgta ccagattctt ttgggcaatc agctcaaatg ccacaaatat
     3121 acagaggttt tggaatagat agttcattat tttggcgtgg agtttctgat gatatggtag
     3181 atacaacaga gtttatatgg aaaggttctg atggttcaaa agtattagca gttcaaatac
     3241 catttggtta ttactatgga ggtaatatac cagaagatga ttcagattta aaagaatatt
     3301 taaaagatat aatcgtaaaa cttaaggtta aagcttctac taataacgta tattttccaa
     3361 atggatttga ccaagcacca ataagaaaaa accttcctga tatattaaaa aaagctaatg
     3421 aaatagataa tgaaaatgaa tacgaaataa gtgatataga aaactatata gattcagtaa
     3481 gaaatgaaag aaatgaattt acagagctta aaggtgaatt tttaaatgct aaacatatga
     3541 gaatacacaa atccatattc tcctcaaggt cagaccttaa aataatgaat aacaaattag
     3601 aaaattatat tacaaatgta cttgaaccgt tattaacatt aagttattca cttggaaatg
     3661 aataccctca cctagttata aaagatatat ggaaacttat gtttgaaaat gcagctcatg
     3721 acagcatagg ttcttgtaat agtgacacaa caaatgagga tgtatacaca agatacaaac
     3781 aagccagaga tttgagtata aatttattag atttgcatat gagacttata agtactcaaa
     3841 tcaaaaataa taatgaagaa ataacattaa cagtattcaa tggattccca gaagtcagaa
     3901 atgaagttgt agaatttgat acttatattc caggtcaaga ttttgtgata aagaataaaa
     3961 atggaaaaat tcttaattat ataataaaag aaaaagaaga tattacaaat tatatattaa
     4021 ctcaaacaat aagattaaac ccaagtaaaa aaatatatat acccaaaact gtatatagag
     4081 caaaaattgc actttcagta aaagatatac caccaatggg atatactcaa ctagtttttg
     4141 aattggatac taaaaataaa tgtgaaatag aaacaagtaa ttgcaaggaa atagaaaaca
     4201 aatactacaa aataacagcc aataaaaatg gttcattaga aatattagat aaagaatcta
     4261 ataagactta taaaaaccaa gctgtaattg aagacaatgg agatgatgga gattcataca
     4321 actattctcc tccaagaaaa gatatttatg tttcatcttt agattctgta tcttcagtag
     4381 aagtgctaaa atcaaacatt caagaagaaa tgatacttaa atattcattg accataccag
     4441 cttcactgga agaaagggct ataggtaaag tttctgtgaa aatgccagta acaatgaaaa
     4501 tcaccttaga acctaaagaa caaataatta agtttaatgt taatataaaa aataaccaag
     4561 cactatctca tagagtaaga gttttattta atacagaaat agcatctaag ttttctatag
     4621 cagatcaaca atttggaata atacaaagac caacagtatt agaaaaagac cttgcattgt
     4681 ggaaaagaga caattactca tggcaagaaa aacctataac tatcgaacca atgcaaagtt
     4741 ttgttacttt agaggatgga caaagaggaa tagcattaat aactggttgt gtaagagaat
     4801 atcaaatagt tggtgataat ttagatacaa tagcattaac actattcagg tcatttggat
     4861 atatgggaag agaaaattta ctttatagac caggaagagc ttctggagaa aaaataatag
     4921 caacaccaga tgcacaatta cttaaagagt taaactttga ctttggtctt tacatatata
     4981 acaataaatt tgatgaagca aatgtagcta atacagctaa gagattctta acacctatac
     5041 aaatatatga atatgctgat tttttaaatg gtagacttat atttgcattc aatgacgaaa
     5101 aacaaatata tgaaaatgaa tttagtttga tgaatgtaaa taattcaaat ttcacagtaa
     5161 gtgcaataaa gaaagaagaa aaaggagatg gaattgtagt tagaatcttc aatggtatga
     5221 aaaatgagga tgctaaggga agtcttaaca tcaataaaaa tgtcaatgat gcttatagtg
     5281 caatgctcga cgaaatctat gataatacta gcaagcttaa agtaaatact aaaactgtag
     5341 aggtaaattc tttatcacat tgtaaagtac aaacaatagt aattaaataa tataaaatag
     5401 cttatacaag atttcaaaat aaattgctag taccatgtta atagtaaagc cagatattat
     5461 gctatttata agctctgtaa cactaaataa cattttgtaa aataaaaagg tgaaccttgt
     5521 atattaggat tcaccttttt taaataaatc aacattctgt tttaaaataa catttataag
     5581 ctgttttgaa ataatgctta caaattattt taaaacaata cctataaatt tttatttaaa
     5641 gaatacacat aaatatttcc agactctttt atcctataga ctttataatt attaaagaag
     5701 tctaatctaa tttttgttaa aagtaattgt aactttaaat aaatcaccat ccacacttat
     5761 attcatctta ccaccctgta attctataaa actttttgct atcgctaaac caagaccaga
     5821 accttctgtg ttacgtgatt tatctcctct tacaaatctc tccattatat catctacact
     5881 aaaatcaatc tcagatgcag acatattttt aagcataata tttactttat tatctgtttc
     5941 taaaatatca atatacactc ttgaatttgg cattgcatat ttgcttatat ttattataag
     6001 attttcaaat actctaaatg tacgctgact atctaatggt aatattattt tttcattaga
     6061 aaagttattc tttactatta gagatgaatc tgcaagtttg tcatctaatt ctaaaagtgt
     6121 ctgcttcatt agagatacaa tatccacatt tactatattc aaataaacat ctccactatt
     6181 tgccttgctt acttcaaaca aatcttctat tagatgttgc aatctttgtg actttctgtc
     6241 taatgtatca atatacaact tacgattttc atctgttata ctttcatctt ttaataaatc
     6301 tatataagtg attatagatg ttagaggtgt ttttaagtca tgtgatacat ttgatataag
     6361 ctctgttttc atcttttgac tcttaacctc ttcgtcaaca gcttttttaa atccagtttg
     6421 aatatttcct aattcgtctt ttataggatt aaaaaatcct aaatcttttt ccatatttac
     6481 atctaagttt ccatctgaaa ttttctttgt aacatctaaa agttgattat attcatatat
     6541 tgtcttttta gagtaatctg acagtatttt aaataatact actgaatata ttaatgcgag
     6601 tattattcca aagaaccaca tactacacat tataactata atcaataaat ttattcccaa
     6661 aattgtaact atagttttat ttggcttttc ttttagattt attgttctaa tttgattaat
     6721 aactgttttt cctgcttttt ttagccatac aaaaaacatt cctgtcacag tatgttttgt
     6781 aaaatactct cttattccaa catttaaaat atgttttaaa agtattataa atacaaatat
     6841 tattgagaat attacaaacc aataagttaa attcattaaa cttaccaata tactagccat
     6901 tttagcactt atgtcattgt ttataaggta ctctacataa ttcccactca aggtattact
     6961 tattataaca gtaggatata aatctacaag cccctgtata atagcaagag caataaagag
     7021 tatctctaac ggtattcttg ataatgtatc aaatcctagc aactctttag attttctata
     7081 tgggaatgct aaagctacaa taacaactat agctaatata atttttaaaa tcattccaga
     7141 aatcttataa taagaattat tttctacatt
//
LOCUS       DAYAOY010000149         7139 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYAOY010000149 DAYAOY010000000
VERSION     DAYAOY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.153"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            226..1050
                     /locus_tag="ACGI6B_003709"
     CDS             226..1050
                     /locus_tag="ACGI6B_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFG9753116.1"
                     /translation="MNWIFSLTPALLGITNTILGEIIFYLAYTSPAIVGLFFISTIYP
                     QSAKQDFIYRYLSFKEIGIKWILLTILFFALTFITSLIIGNYFNVKTSDIDWIHIAVF
                     RPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVRFGFIGSSIILGLVCGIWYLGSYF
                     TPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVYINTSRSILSAGLVHMMYYFFNIQ
                     LLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFKQKVNSEIEQIKSDDFEFELDW"
     gene            1228..2052
                     /locus_tag="ACGI6B_003710"
     CDS             1228..2052
                     /locus_tag="ACGI6B_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9753117.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2178..2759
                     /locus_tag="ACGI6B_003711"
     CDS             2178..2759
                     /locus_tag="ACGI6B_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9753118.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2863..3039
                     /locus_tag="ACGI6B_003712"
     CDS             2863..3039
                     /locus_tag="ACGI6B_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753119.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4305..5057
                     /locus_tag="ACGI6B_003713"
     CDS             4305..5057
                     /locus_tag="ACGI6B_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753120.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5047..>7139
                     /locus_tag="ACGI6B_003714"
     CDS             5047..>7139
                     /locus_tag="ACGI6B_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753121.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFK"
BASE COUNT         2661 a          950 c          945 g         2583 t
ORIGIN      
        1 attatattta gtgtaaaaat aattaatcaa aatataaaaa ataggatatt attataggca
       61 ataactaaca tcaagcgata acttaataca tataaaaata aataaacaat taataaaaat
      121 taaaacttca ttataaaacg gaaggatagt gaaaaaatgc aactatataa cacccccttt
      181 aatacaaaca aacacttaat ctatttcttt acatttactc tagtattgaa ctggatattc
      241 agtcttacac ctgcgttgct cggaattaca aatacaatac ttggagaaat cattttttat
      301 cttgcatata catcaccagc tattgtaggt ctatttttta tttctacaat ctatccacaa
      361 agtgcaaaac aagattttat ttatagatac ctaagcttta aagaaatcgg aataaaatgg
      421 atactattaa caatattatt ttttgcttta acttttatta cttcgttaat aatcggaaat
      481 tactttaatg taaaaacttc tgatatagat tggatacaca tagcagtgtt tagaccttct
      541 aaaatagctt atatgctatt agcaagtttt atattgagta tacttagtca agaagctgga
      601 tggagaggat atgccattga caaattatta gttagatttg gatttatagg ctcttcaatt
      661 attttagggc ttgtttgtgg aatctggtat ctagggtcgt attttacacc taatcaaata
      721 ccatataatt tggcacaata ctccctattt gatgcttttt tatttatacc aaacattgta
      781 cttttaaact ttataattaa ttttgtatat atcaatacaa gtagaagcat tttatcagct
      841 ggattagttc atatgatgta ctattttttc aacattcaat tacttctaca ctaccctatt
      901 aaattatgtg taatagctca atatgtacaa ataatatttg gtttaatatt tcttgtatat
      961 atgatttctt ctaataaatt taaacagaag gtaaattctg aaattgaaca gataaagtcg
     1021 gatgattttg aatttgagtt agactggtaa ttttaatatg aattataaat taagataatc
     1081 tatgatttga tacgattact acttaaatat aatttactct ctataattta ttgcaacatc
     1141 tggaaaataa ttgtatttcc acaaacgctt gaagtatata aaaacaaaat atatactgat
     1201 tataagtgaa aaggaggtca tattttcatg tttaaaatag gagaggtttc aaaactaaca
     1261 caaatttcta ttcgtatgtt gcgttattat gatgaacttg gtattttaaa acctgcaaaa
     1321 acagacaaat atacaggtca tagattatac tcagttgaac agatttcaat attacaaaga
     1381 attgtattat tgagagattc aaaatttagc gttgcagaaa tagcaaatat cgtacataat
     1441 tggaatgatg aatttgtaat taaggaattg aatagaaaaa agaatgaaat acaaaaggaa
     1501 ataaagcagg aacaacaaag aataaataaa attgataaat ttatagaagc tataaactgt
     1561 gataaagatg aaattcatta caatgttgtc tttaaaaaga ttccaagtta taaaatcata
     1621 tcacttagag agataattcc agactaccaa tctgaaggta tactctggga aaagctatct
     1681 aaatttatca aagaagaaca tatagaagta tcacgtcaat ccaacaataa catagctttt
     1741 taccatgatg aagaagtcaa agataatggt gtagatattg aagttggaat ggttgtaaag
     1801 aagattggaa agaacaaatc tggatttata tatcgtgaga cagaagaaat tgatatgatg
     1861 gcatgtacta tggtttatgg tccatatgaa aatatagctg gtgcatatga atcgttctgt
     1921 tattggttag ataaaaatag tgattatcaa atatcaggta taaatagaca aatcggacat
     1981 aaaggtgaac ataatgaaat aaatcctgaa aattatctta cagaaattca aatccccctt
     2041 ataaaagtct aatagattag tacttaaata cttaagtact aatcacccat tgacatccca
     2101 aaattttcat gatagtatct aaacgtgtat taaacatgtt taattttttt atggagaaac
     2161 ttttaagggg aaaaattatg aaagcagatt caacaaagga acaattaatt aatgccacaa
     2221 tccagttact atcttcacat agagatgttt caagcattac ggctagagaa attgtagcta
     2281 aagcaaatgt caatttagct atgattaatt attattttaa atctaaagag gagttaatta
     2341 atatttctat taaaaaattt ttaaagcgtc atatggatga atataaggct acacatgata
     2401 ctaaaaattt agcacctaaa cagcaattaa gggaaatact aattgcattt tttgatttta
     2461 taggtgaaca tagtcaattt ataaagattt ctgtacccta tatattgata caggaagaaa
     2521 ttgtttatcc atttgagata ctacctttaa ttaaggctca ctatgaaaat cagaataaca
     2581 aaaaaactga agctgaatac agaactattg cgtatcaaat aacttcattt attcagttgc
     2641 tttttcttcg ttctaatgct tttttcaagt atagtggtat tgatattctt aatattgaac
     2701 agagaaatgg tttaatagat tttcaattag atttattatt ggtagatatt acaaaataat
     2761 actatattaa ttttttatgt acattttttt attttatggt aacgtttgaa aatattctca
     2821 ttgactaaaa atacttgcat taaataaggg ggaggaattt taatgttttt tacatttata
     2881 ggagtaatat cagcaatttt attgttgtgt acaattatat gtggtttatg gataaaactt
     2941 aataatcgta ataatgactc taattctcac tttacttttt caatactttc aattgctttt
     3001 tcactgttaa ttgtggtggc ctttttagta agaaattaat gtctttgtta tgctgaggtt
     3061 tattattata gatttttatt attgcatgat ttttactata ttatagtatt tttattatat
     3121 tacagggtct tatttattat agtgttttgt tgttacagca ttttattata ttacagagtc
     3181 ctatttatta taggattttt attatattgt aaaatcttat ttattatagg attttgttat
     3241 attacagact tttattatat tgaaatctct tatttattgc agtgtttcta ttatattgca
     3301 aactcttatt tattatggtg ttttgttatt gcaacatttc tattatattg taaagtctta
     3361 tttattacag ttctttatta tactacagac ctttattatt acagcatttc tattacattg
     3421 taaagtctta tttattacag ttctttatta cactacagac ttttattatt acaatctctt
     3481 atttattaca gttctttgct atactacaga cctttattat tacagcattt ctattacatt
     3541 gcaaagtctt atttattaca gttctttatt atactacaga cttttattat tacagcattt
     3601 ctattacatt gcaaagtctt atttattaca gttctttatt atactacaaa cctttattat
     3661 tacaacattt tattatttta acttctttac tataatatag taatatttta atatctcaaa
     3721 atcatatcaa tcacttttca gcatagtcta aaatacttcc actttctaaa attatctatc
     3781 atacctatac taataatact ttttaacctt tttattatac atttcaattg tttctaacaa
     3841 cctatttatt tccaaaactc caatatattt tctcaattac attgtgttaa tagcccctaa
     3901 atcctttcaa attatttaca atacattcca ttaaataatc ttactgctct ataattcttt
     3961 ttttcttttt aaccctacta tacattattt acatcatttt tttcaataca acattcgtta
     4021 ttctataacc tttttacttt tactatatac ttcttttcta attacacctt caatgttcca
     4081 catcatatta ctttagcaca acaattaata cctaaaaata accctctaaa tcctccaata
     4141 atataatcaa tttaactata ataataacct ctcacactat ccaaacaata tattttcaaa
     4201 cattacaaaa aaacatccaa ctattacaat atagctcata tccctcctcc cctttcaaat
     4261 gctatcattg acttacaaga taaatgaaag tgaggatgat atatatgctt aaagtaaacc
     4321 aattatgtaa atcatataaa acaggcagta aaacatatcc tgtattaaaa aatatttctt
     4381 ttgaaatcaa caaaggagaa tttgtcgcaa tcatgggttc atcaggaagt ggaaaaacta
     4441 ctcttttaaa ttgtatttca tgttttatcc caattgataa tggggaaata attcttggaa
     4501 ataataatat aagaaattta aaagaaaagg aactttcaga agttagaaat caacaactag
     4561 gatttgtatt ccaagacttc atgctatttg atggtcttag tgtctttgaa aatatatgtg
     4621 ttccacaaat tatcaaaggt agtcaagtct tacaaatgga aaaccgtgca attaatctat
     4681 gtgagctatt tggtattgaa catatcagaa aaaaataccc tgccgaaata tctggtggag
     4741 aaaaacagcg tacagcagta gcaagagcac tcatgaacaa cccagattta atattagcag
     4801 atgaaccaac tggtaacctt gatagtaagt cttgtcgtac agtcattgaa tcatttttaa
     4861 aagcaaagca agaattagat gctactatac tcatggttac acacgatagc tttgcagcaa
     4921 gcttctgtga ccgagttatt gtgcttaaag atggaataat ttataaagaa gttgtaaaat
     4981 caaattcaag acataaattc ctagatgaat tactagaagt tttaaaagaa atgggaggcg
     5041 ctcaagatga caattaataa attatacagt aaacttcgag cctataattg gaagaactat
     5101 acaactttat tcttttgtat aatactttct gtggtattag taacttcata tgctctaatt
     5161 tattttagcc caacaataca aaacatactt ccaactggtg gagatagcag aaaacaggct
     5221 acaatgatat tctcagttgc tatattagga tgcgctgtct ttactacata cgcttcttcc
     5281 ctctttttaa aatttaaaag caaggaaatt ggtattttca tggctctagg aacagataag
     5341 aagaaattaa aaaaacttct attttcagag atattgttaa tttcaatagt atgctgtttt
     5401 ttaggtctta tattaagtat tcctgtatct tttggtatat ggaaaatatt tcaattagtg
     5461 attattgata ccagagaaat gacatatcat tttggaactc aaggattcat atatggattt
     5521 atatttagtg tatttgtgac attatgcata tttatacttt ctgtaaaatt tattaatcgt
     5581 acaaatataa ttgatatatt aaatgaccag agaatatctg aaccaataca ggatgtaaag
     5641 gactggtatg gtatattagg tataattttt attatactcg gtatttttct aggatatctc
     5701 gtaccttcac taactataaa caaattaaat tataaactac catctatatg gagctttact
     5761 tatctactct ctcttattgg aatatatatg attatgacat acatagttgt acatgctaaa
     5821 agaggaaaac atccagagaa atactataaa aacatcatat caactagcat gatgagattt
     5881 atgggaagac aaacagtaaa aaacatgtgt gttatttcat ttttgattgc aggagcatta
     5941 tttgctgtat tctatgttcc taatatggtt acaggtatct atgatactct taaaaataac
     6001 cctatagatt atacttttac ctacaatcaa agagataaac aggtatcaaa aaaagatatt
     6061 gaacaattag cagataaaca caatataaaa attcaaaact atatggaaat tccttctata
     6121 gaattaattg ttgatggtgt agaatcaata tatcataaag atggaactat cgaaaaggta
     6181 tatatgaaaa aaagctccta tgatatgttc tttaattcca aagatttttc caagctaatt
     6241 ggcaaaccaa taactataaa aaatggtgaa tatcttgctg ttgtaaataa aggaaatgaa
     6301 aatcaagatt ataaatatac caaatttata tcaagcccag tcacagaaaa agaaatcccc
     6361 tttagatatg ctggaaaaat aatatttaat ggttcttttt ttcaaaatag aagtatgaac
     6421 gcctatgtcc ttaatgagga ggattataaa tcatttacta aagatttatc cataaaaaat
     6481 tacaataatt ctatactatt taatgtagat aaagatacct atgcttttgc caatgactta
     6541 aagaaagaaa ttataaaaag aacatctaag gaaatgggca tgttcagtgg ttatgatgaa
     6601 tacgtaaaaa aacgttatga atcaactgga agaaaatatt ttatggatga agaatatcca
     6661 agtggtgaag gtcatttaaa actagaccca aacaacaatc agttgtatct taattggaaa
     6721 tactatccta actttaaaat tttacaaagc caagatatga taaaaaacat ggcagtcttc
     6781 ttaatgttat tcatatatgt ggcaatcata tgttttacag cagtaggaat cattgcatat
     6841 acaaggggtg ttactatagc tataaatcac aagcaggtct ttatggattt aaatcgatta
     6901 ggagcaaaca atgattatat aaagttctgt attagaaatc aattaaagaa agtattttca
     6961 taccctctta ttgcaggaag ttttttaatt tatctgttta attttatgat tatgttaaat
     7021 aatgatggta gaatcgtcaa tagtgaggtc aatgctcttt tgataaactt aggttttata
     7081 gggatttgtg gggtttatat gtaccttata tatctattaa cttataagaa attcaaaaa
//
LOCUS       DAYAOY010000150         6941 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAYAOY010000150 DAYAOY010000000
VERSION     DAYAOY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.154"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            86..1342
                     /locus_tag="ACGI6B_003715"
     CDS             86..1342
                     /locus_tag="ACGI6B_003715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9753122.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            1365..3446
                     /locus_tag="ACGI6B_003716"
     CDS             1365..3446
                     /locus_tag="ACGI6B_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFG9753123.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            3483..4568
                     /locus_tag="ACGI6B_003717"
     CDS             3483..4568
                     /locus_tag="ACGI6B_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9753124.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4622..5746
                     /gene="wecB"
                     /locus_tag="ACGI6B_003718"
     CDS             4622..5746
                     /gene="wecB"
                     /locus_tag="ACGI6B_003718"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFG9753125.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5824..6798
                     /locus_tag="ACGI6B_003719"
     CDS             5824..6798
                     /locus_tag="ACGI6B_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9753126.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2894 a          644 c         1156 g         2247 t
ORIGIN      
        1 ttttaagaga atagataaaa agaaattttt aaaataaagg attttatatt agaatcaata
       61 ttttaaaata ttttaggaga aatttatgag agatttaaat gtatcagagt atttgttcgt
      121 atttagtttg tttagtatat ggtctttatt gcttataaat attattttag ctatgggtgg
      181 atatatattt tattttaaaa actttgataa agaaataaaa gaaattgatg aatatcctat
      241 gatatcaata cttgttcctg ctcataatga agctaaagtg ataggtagaa ctgtggagtc
      301 tttgctatta ctcaattatc ctaagagtaa gatggaactt attgttataa atgacaattc
      361 ttctgataat agtaaagaaa ttttagaaaa tataaaggat aggtacaaca attataattt
      421 cactataata aatacagata gtttaacagg tggaaaagga aagtctaatg cattaaatat
      481 tggatatacg atttcaaaag gagattttat agctgtttat gatgcagata acacaccaga
      541 taaaaatgca cttaggtatc tagtacaaac tatagtaatg aatgatgaat taggtgctgt
      601 tattgggaaa tttaggactc gaaataaaaa taaaaaccta ttaaccaaat ttataaatat
      661 agaaacatta agctttcaat ggatgtcaca agctggtaga tggcaacttt ttaatctatg
      721 tacaatacca ggaactaact ttatattgag gagaagtatc atagaagaaa ttggaggatg
      781 ggatagtaaa gcaattgcag aagatacaga aataagtttt aggatatata aattgggata
      841 caagataaag cttgtaccac aatctataac atgggaacaa gagccagaaa ctgttaaggt
      901 gtggataaag caacgtacta gatgggcaaa agggaatata tatgtattaa tgaaatatat
      961 aaaaaatata tttaaacagg gaagaaataa gattgtgttt gatatagctt actttttttc
     1021 tgtatatttt ctatttttaa catctgtaat tatatccgat attttatttg ttttaagtat
     1081 atctaaacta gtagaaattt ctattccaat taactttttt ctaatatgga tactatctta
     1141 tcttttattt attatagaag tgagtataag ccttactata gaaaaaggtg aagcaacaat
     1201 tgaaaatata ttcatagtgg caataatgta ttttacatac tctcaactat ggctgtttgt
     1261 agctataaaa ggaatgatag agtatctaaa agatataatt tttaaaagag aggttaaatg
     1321 gtataaaaca gaaagatttt gattcatgta caggaggaat taacatgaaa aaatttatta
     1381 tatcaattat atctttagta ttgttttttt caaatatatc acttatatac aaagtaaatg
     1441 ctgatgagac taaagttaaa aattataagt ttgaaagaga tataactatt gatggtgtta
     1501 taggaagtaa tagtactttt tttgaagtaa ataaaaattg ggatattgaa gaagttttat
     1561 tgcatcttaa ttttagtaag agccaaatat taaatggaga tgtttcaagt ttaactgtat
     1621 tgataaataa tgtaccaata aaatctatta aattaaatgc taagacaaat tataaaaata
     1681 cattagaagt attagttcca aaagattata taattcaagg atataatgaa ataaaaataa
     1741 aaacatacaa aacaatatct gataaaattt gccaagatga ttccaatact gggaactgga
     1801 tggttattca caaagaatca tatatatcaa ttagatataa gcagaaaaag gtggaaaact
     1861 caataaatga atatccgtat ccatatgcag aaattgaaaa taatcataaa ttagatacaa
     1921 ctatagtagt accagataat atgactagag gagagactac agcagtgttt aatttggcat
     1981 cagcatttgg aaaaataaca aaaaatgatg atttaaagtt agatgttaaa ttatattcag
     2041 aaatgaaaaa ttggtcagat gataatatta tatatattgg taaacctgaa aatacagcag
     2101 aagaaatttt ggatatttta agtataaagg aacaaaccct attatcttca aactgtataa
     2161 tcaagcaagt agattcacca tacaataaga ataagaagat gatggtggta attggtagta
     2221 atgaagatga tttaataaag gcatcaaatt tattaataga aaatagactt tctaaccaag
     2281 ttttatcatc atcagtactt gtaaacaaag agactaatat aaaaattaat agggaacaaa
     2341 aattaaattt aggtcattta acattaaaag atttaggata ttctgacttt ttgttggaag
     2401 gagcatttaa tcaacaagct ttgtttgatg tcaaaatacc aacgggtaag gtattagatg
     2461 atggttcaaa gattatctta aatttaagat attctgacaa tttagatttt gaaaaatctt
     2521 tagttacagt gtctataaat gatgttattg tgggaagtaa aaaattggat aggtcacatt
     2581 ctaataatga taagttagaa ttaaaaatac ctaaagatat agataataaa aactattatc
     2641 aagttaaatt gacatttaat ttgagtataa aaaattcgaa ttgtgttact agagagagta
     2701 ataatccttg ggcatatgta tcaaataatt cttatttggc gttatctacg aaagaaaatg
     2761 aaaccttatc ttttgaaaat tatccgtatc catttgttag agatgatgaa tttaatgatt
     2821 taactgtgat aatgccagat tattcagggt cacaagctat gacctggatg tttagattag
     2881 gtgttacttt aggtgcgaat ataaattcac ataatggaaa tataaatgta attagaggaa
     2941 aagaatttag tgacaagtat aaggatacta atattgtagt ttttggagtg ccacataata
     3001 attctgttat aaagatgtta aataataatt taaatataaa gttcgataaa aattactcca
     3061 attttatatc aaatgataag ataagcttta ttgatgatta tggaaaaaat atttctacta
     3121 ttcaattaat aaaatcacca tataataatc aaaaaaatat catggtaatt agttcaatga
     3181 atgaaaaaaa cttatattta ggaatggatt atttgttgaa taaaagtaaa gttaatgatt
     3241 taaaaggaga tacattgata attgatgaat acggagaggt agaagattta gcttataatt
     3301 taaaatctaa gaaagaagta aaagattcaa gttggaatat gagtataaat aagacaacta
     3361 aagtattttt aatgatttct tttattacta taattatagt catgatatta tcaatgctat
     3421 atataaaaaa atataaaaga agataatatg ataaatttat tattttaaaa gaggtatata
     3481 atttgaggaa atatatgaat gaatttaaat taaaaagaag tattttaatc tcattaataa
     3541 tcttaatttt tatatttata gtttactttg taagtattga gttaaaagat aggagtctag
     3601 taaataatca atataaaggt aagattaaat cggccaattt atctgtagat tatgaaataa
     3661 atcaagttat gaaggatata gataaattgg gattgaatac agtaaatgta cctataatta
     3721 taaatgtaga gtccattaat tctgatatta tgtcaataga taataatagt aaagaaaagg
     3781 caattaagtt aataaaaaaa ttaaataaaa aaggtatttc aacaatatta gaggcatatc
     3841 catggattga aaatggaaaa ttgtatgaga ctgactggaa ccctataaat aaaaaaagat
     3901 ttttttatac ttggcaaaat gccatattaa atgaacttat aattgatgta gcaaatccac
     3961 tggatgtaaa tgttttaaat attggttcta attttgtaca tttggaagaa tatcaacaaa
     4021 attggggtga aataatagat tttgtacaat ccaaatttga cggtcttgta acgtacagaa
     4081 ctaattggtg gtatacaaaa aaagatgatt taagtagtaa gttgttttat gaacaaaagc
     4141 tgaacaataa tttttttgat aagttagatt ttatatctat agcagcatat tttgagttaa
     4201 gtaataaacc tgttaataca gtggatgaat taatttctgc cttacactca tctactgtaa
     4261 acaatagagg acaaaatata aaacaagaaa tatataacct ttataaagct catagaaaac
     4321 ctatattttt tggagaatta ggtttttcaa atagagaaag tgcaagttct cagccgtgga
     4381 atcatactcc ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt gaggcatata
     4441 agaaagtttt tgaaaatgaa gactggataa atggtttttc tgtgttttgt gtaggtaaaa
     4501 tagatgacga aaaaaatttt tacccatcaa aagagagtat aaaagttatt aagtcttggt
     4561 atgaataatt ttgaaagtgt agaatttata tagattaaat agatgattag gagagatata
     4621 aatgaatgac atcaaggtta tgactgtatt tggaactaga cctgaagcta tcaaagtagc
     4681 acctttaata aaggaacttg aaaaaagaga gaatataaaa agtattgttt gcgttacagc
     4741 tcaacataga gaaatgcttg accaagttat agaaaccttt aatataaatg tagactatga
     4801 tttggatata atggaaaaag gacaaagcct gaatgatata acatgtaaga ttttaaataa
     4861 acttcccttg attttaaaca aggaaaatcc aaatataata ttggtacatg gagatacaac
     4921 cacaactcta gctacaagtt tgactgcatt ttataataaa actcttgttg gtcatataga
     4981 agctgggctt agaacctatg ataaatattc tccttttcca gaggagctaa atagacaact
     5041 tacaggtatt attgctgata tgcactttgc tcctactaat ttggctagaa aaaacttgat
     5101 ttctgaagga aaacctaata ataatatatt tgtaactggt aatactgcta ttgatgcttt
     5161 aaagatgaca ataaaagaaa actataatca tcctataatt gatgagattg gtaacgatag
     5221 gatgattttg ttaacctctc acagaagaga aaatttgggt aaacctatga aaaatatatt
     5281 cagagctata aagcgaatag tagatgactt tgaagatgta cagatagtat atcccataca
     5341 tttaaatcct aaaatacgaa ctatagctga tgagatattt ggaaaatttc ctgaaaaaat
     5401 tcatattata gagcctttag atgtagctga ttttcataac tttttaaata aatcttatat
     5461 gataatgact gatagtggag gaatacagga agaagctcca tcattaggta agccagttct
     5521 tgtgttgcgt gataaaacag aaagaacaga aggaattgaa gcaaagacac ttaaattagt
     5581 aggtactaat gaagatagaa tatataattc agtgagcgat ttattaataa ataaagataa
     5641 ctatgttcag atgagtaaag catctaatcc atatggagat ggaaatgcaa gcaaatatat
     5701 agtggatata ataattaaaa aatttaattg caaatattta aattagataa ggcctaaaac
     5761 attaactaat tgatataaga tttatgataa tattaaggaa tacaattttt aagggggagt
     5821 aatatggcta gaaaattaat aaaaaatttg ggtaaaagta aaagtgtaaa gagagtgaaa
     5881 cttttattta agaagatttt tattactgta ttcatagtag caagtatagt tgctattttt
     5941 aatataacga aatattttga ggaattatac aaagtaaggg atttaaaaag tactaaaatt
     6001 gaatactata tggatgtggc tgatgaagca ggagatggaa aggtccaatt aagttggaag
     6061 gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat aaaaaagaaa
     6121 gacacattgg atatagggga aaagtttata gtagaagata aaaatggtaa aggcgaaaag
     6181 gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga ctcttctcaa
     6241 aaaagtagag caagaaaata catgaaagat ttagaataca cataccttgg aaataaacaa
     6301 ttagatagta gtgatgaaaa aattaaattt ataaagaagt tagaagactc agctataaga
     6361 gaatatattg attatggaat attgccctct ataacaattg gacaagctat attagaatct
     6421 ggttggggaa attctaaact tacaaaacag agtaataatt tatttggtat aaaagcagat
     6481 aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa tgaaaaaatt
     6541 gtagctagtt ttaggtcgta taattcatta caagattctg tcaaagacca cagtttattt
     6601 ttaattaata ataaaagata tagaaaacat gggttgtttg aggcaaaaga ttatattagt
     6661 caagctcaag cattagaaaa tgcaggatat agtacagcag aagataaaaa aggaaatcgt
     6721 atatatgcag agctattaat agatgttatt aggagttata atttgcaatt gatagataat
     6781 aaagttgaga caaaataatt gttaacttta tatgaggaga taacaagata agattttttt
     6841 ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac ttgttattta
     6901 aaaggtgata ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DAYAOY010000151         6933 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYAOY010000151 DAYAOY010000000
VERSION     DAYAOY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.155"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..707
                     /locus_tag="ACGI6B_003720"
     CDS             <1..707
                     /locus_tag="ACGI6B_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9753127.1"
                     /translation="RDKQVPQGRILNATISKEPSGRYYVSLCCTNVDIEVFENTNNQI
                     GLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARL
                     QEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQL
                     KYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDLNIREWICPSCNETHDRDINAS
                     INILKEGLRLITIQNK"
     gene            828..1235
                     /locus_tag="ACGI6B_003721"
     CDS             828..1235
                     /locus_tag="ACGI6B_003721"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFG9753128.1"
                     /translation="MANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(1535..2122)
                     /gene="rbr"
                     /locus_tag="ACGI6B_003722"
     CDS             complement(1535..2122)
                     /gene="rbr"
                     /locus_tag="ACGI6B_003722"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9753129.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(2208..2645)
                     /locus_tag="ACGI6B_003723"
     CDS             complement(2208..2645)
                     /locus_tag="ACGI6B_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9753130.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            2749..3747
                     /locus_tag="ACGI6B_003724"
     CDS             2749..3747
                     /locus_tag="ACGI6B_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HFG9753131.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3867..4793)
                     /locus_tag="ACGI6B_003725"
     CDS             complement(3867..4793)
                     /locus_tag="ACGI6B_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFG9753132.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            5178..6782
                     /locus_tag="ACGI6B_003726"
     CDS             5178..6782
                     /locus_tag="ACGI6B_003726"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9753133.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2616 a          849 c         1102 g         2366 t
ORIGIN      
        1 taagggataa acaagtacct caaggtagaa tacttaatgc tactatatct aaagaaccaa
       61 gtggtagata ttatgtatca ttatgctgta ctaatgtaga tattgaagta tttgaaaata
      121 ctaataatca gataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag
      181 actttataga aaatcctaag tatcttaaga agtcattaaa caaacttgct aaattacaaa
      241 gagaattatc gagaaagaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg
      301 caagacttca agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta
      361 aattaataaa agagaatgat attatttgca tagaagattt acaagtaaag aatatgatta
      421 gaaatcgtaa actatctcgc ttaatttctg atgtgtcatg gtcagaattt gttcgtcaat
      481 taaaatacaa ggctaattgg catggtagac aaattgtaaa ggtaggtaaa ttctttgcaa
      541 gttcacaaat atgcaataaa tgtgggtata aaaacgagga aatgaaggac ttaaatataa
      601 gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat gcaagtataa
      661 atatattaaa agaaggatta agactaataa caattcaaaa taaataactt atgtaagaac
      721 cgtaggaact acggggatag cctgtggaga attagtaaga catattttag tatgcaaaat
      781 tctatgaagc aggaaccctg tgactttagt catgggaggt tcaggatatt gcaaatatgt
      841 ttatgaaaaa ggatattaat aaagaatttg acattgattt tattctcagt aaagaagtta
      901 tatgtaatta taccaaagaa atatttcaaa atttatataa aaaagaacaa agcataggta
      961 aaaaagaact gcttatattg agcataatag agaagttttt agttaaatct ataataactt
     1021 ttgaagagtt aaaattgata gtgaatataa tagaagaaaa ttttacatca atatacaatg
     1081 aaagtggaaa gatttatttg tttagaaagt ttggagttcc attcgtagta ggatgtctgg
     1141 attataaaaa agtaaagttt gaaaaagatg tagttaagat tattgaggtt gacttgacaa
     1201 aagaaataaa tgatataagt ataaagttat catagtgtat tatcttatat aatgtattat
     1261 aataatctac ttaaaataca ctttatttaa atatttagtt taaagatgag attttatgtg
     1321 agtgtgtaaa agttaaagat agatttaagt aggttatagg gattagtaaa caataaaatg
     1381 aaccatgatg aactactata aattgtattt aaactacatt ttctacttat agtaatatca
     1441 atggttcatt ttattgttat taagataata cagtatttat aaaaatactt gatataaata
     1501 tagtaaattg tttaaataga attattgcct taatttattc aaataatgag tttttaaaat
     1561 ctaagaaata tccttgtgga tgaagacata caggacaagc tttaggagct tctttaccta
     1621 tatgtatatg gccacaatgt gtacatatcc attgtatatc ttcatttctt ctgaataaag
     1681 taccttcttc taaatcagta gcatatctac caaatctatc accatgaact ttttctattt
     1741 cagctatatt ttcaaataac ttagctatag attcaaatcc ttcttcacgt gctgtttttc
     1801 caaagttttt atatacatca tcccactctt gaagctcatt atgttgtgca gctttaaggt
     1861 gttgtgctgt cttgtcatat aaatctacag ggtatgctcc tgatatactt atattttcac
     1921 cagcaaattc ttttaattta gaaaagaatt gttttgcatg tgatttttct tggtcagcag
     1981 tgtaattaaa taaagactct attatatata atccttcttt ttttgctaca gatgcagaaa
     2041 tattgtatct atttctagct tgactctcac ctgcaaaagc tcttaataaa ttttctttag
     2101 ttttactatc ttttaaattc atattctacc tctctatcac aataaatttt tacaatattt
     2161 gattcttttt atttattatt ccaataggag atataattat gctcaattta gtcagcattt
     2221 ttaaaattat cattaatttt ttttaaaatt ctaagaaatt cttgtttttc attttctgta
     2281 atattatggt atgccgtctc tttaacttgt gttgagatgc tattaaaagt atcttctata
     2341 agcagtgcct tttttgtaag ctttatatac gtgatacgtt tgtcatttgt acatttttgt
     2401 ctttctaaat atcctaggtt tataagttta ttaaccaaca ctgtaacagt ggatttatct
     2461 tttcctattt tagctgcaat ttctttcatt gtaagtattc cattattttc ataaagtgca
     2521 gttaaaatat ttccatgagt aggtattaac tcacctaaac catttttact gagtttattt
     2581 tcaatgaatt taatcatact agcctttgtt ttacttatga aatgtacaat atatttatct
     2641 tccataaatt attctccaat ctttaatcta tttaaattag tttgacataa aactatttat
     2701 aaactataat tagtttaaca taaaactaaa tgtattggga ggaaatttat gaataaaaaa
     2761 tttaaatcac tatatattac agaagaagaa ggaaatttta gaagaaatat agttgaaaga
     2821 gaagtggata cattgccaga aggcgaagtt ctaataaaag taaaatattc ttctctaaat
     2881 tacaaggatg cactttctgc aaatggtaat aagggtgtta cgagaaagta tcctcataca
     2941 ccaggaattg atgcatctgg tatagtagaa tctagtagtt gtgatgattt taaaattgga
     3001 gaagaagttt tagtaacagg ttatgacttg ggaatgaata cttctggtgg tttttcagaa
     3061 tacataagag ttccttctag ttgggtagtt aaacttccta aaaatctttc actaaaagaa
     3121 gcaatggatt atggtacagc agggtttaca gcagctttat ctgtgtataa atttatagat
     3181 tctgtagaca agaatatggg agatgtatta gtcactggtg gtactggagg agttgctgta
     3241 attgcaacta aaatattgat taaattaggt tattcagttg tagtttcaac aggaaaatta
     3301 gaagaacaaa aagaagtatt actaaactta ggtgttaaag atgttataca taggtcagaa
     3361 gtggatgata actcaggaag acctctgctt agaccaagat gggctggagt aatagataca
     3421 gtaggaggaa atactctagc aacagcaata aagacaacta attattgtgg agcagtaaca
     3481 acttgtggta atgcaggagg agttgatttt acaagttcag tatatccatt tatcttaaag
     3541 ggaattactt tatatggtat agattctgtg gattgcccaa tgaacttaag attaaaaata
     3601 tgggatttat tatcaaatga atggaaatta gatgatttag atgatatggt aaatgtagta
     3661 tcattggaag agttaaatga tagtatagat tcaataatgg caggaaaaca tgttggaaga
     3721 acagtagttg atttagaaaa aatataggtg aataaattta taattaggat tataggtttt
     3781 aagcaaaaat gagatacttg aaatttactt gtgagtatct cattttttta tgtaaaaaga
     3841 tttatgtaga tgtttgtgta aaaagattat tcatgaacaa ttaattcaaa tacatctggt
     3901 cttgaatagt gaccttctga atcaaaatct agtctactaa gtactatttt ttctaggtct
     3961 aaatcagcta ttagcatttc ttctttattg aaaataggac ctgctacata ttttccaaaa
     4021 gggtcaacta tacaacttcc tcctggacac ataatttctg gttctgcatc tagctcttta
     4081 tagtagttaa ggtcagttgg atacatattt ttttcaacat attgattgca tccaattaca
     4141 aaacatcttc cttctaaagc aatatgcttc atagttgctt gccactcttc tcttgaatct
     4201 gcagtagggg ctatgtaaag ttttacaccc ttagcataaa gagctgttct agctaatggc
     4261 atataatttt cccaacaaat caacgaaccc atttttccat atggagtatc aactactgta
     4321 agagtacttc catctccttc tccccaaata catctttctg ttcctgtagg tttaagtttt
     4381 ctgtgtttac caattagctt accttctgga gagaaaaata agtttgtgca ataaagggta
     4441 caatttatat cattaccatc tctttctgtt atacccattg atagatatac tcctgcttct
     4501 tgtgctgctt ttcctaataa atctgttgta gcactaggaa ctggtacaga attatcatag
     4561 tatcttttcc aatcttctct accttccatt gttctacttc caacaacaaa tccaaatgaa
     4621 agacctcttg gatatgcagg tatgaatgct tcagggaaaa ctacgatatt tgcccctttt
     4681 ctaccagctt ctttaattaa tcctaatgct ttttcaacag ttttttctaa atccattatg
     4741 actggtgacg cctggacaac agcaattctt acatttttat tttccttgct cataaaaacc
     4801 tcctaaaaaa tatttaaatt ttttgcattg aatagattac atgagatgtt atttgagaac
     4861 taagatactt atattgtgaa taataatgta actgtatatt taaaataata catcatgatt
     4921 taaaaaatta caattgtaca gatataattg agtttagata tttaaattta acaacttata
     4981 atattttttc aattagtata aagcctatta atttgtctta tttacaatga atagtattta
     5041 cttcttggca aacaataatt aaaaataaaa cttttatatg attaaactct aaagaaaaag
     5101 tttataaaga agataatata attaagcaga aaataatgaa aaaaatttat taaagtagac
     5161 aacgtaagga gaattttatg aaagaaaaaa tttattacaa tggaaatatc atcacaatgg
     5221 aagattcaat atgtggagat gctattctta taaaagacaa aataataaaa aagataggaa
     5281 caaaagaaga agtatttgct ttgaaaaata aagatacaga aattatagat ttacaaggta
     5341 agacattgat gccatctttt atagattcac atagtcactt gatagctttt gctactactc
     5401 taaagctagt tcctttagaa gatgcaacca gttttaaaga catagtaaaa aaaatacagg
     5461 atttcaaaga gtcgaataat ataaaaaaag gagactggat aattggattt tcatatgata
     5521 ataatttttt agaagaaaat aagcatccag ataagagtgt actcgatagt gcatcttcag
     5581 aaaatccaat actaatttca catgcttctg gtcatatggg agttgcaaat acattggggt
     5641 taaaacagct tggtgttacc aatgaaacac gtgacccaga aggtggacat ataggtagag
     5701 tagaaggcag tgaagaacca aatggatatt tagaagaaaa tgcatttttt aatgtagcat
     5761 ctaaaataaa acaaccatca tcaaatgaga tttttaattc aattgaaaaa gcacagaaca
     5821 tatatctaag ctatggtatt acaacagctc aagaaggttt aatggaagaa aatcaattta
     5881 acattttaaa agcaatggca aatcaaaata aattaaaaat ggatgttgta ggatatgtaa
     5941 atttgaaaaa aagtaagtct gtagcagata ataataggga gtttataaaa aaatatataa
     6001 atagatttaa aataggaggt tataagattt tcttagatgg ttctccacaa ggtaagacag
     6061 catggttgag tagaccatat gaaaattcag atgatggtta ttgtggatat cctatataca
     6121 aagatgaaga agtagaaaag tttatagata tttcactaaa agaaaagatg caactcttaa
     6181 ctcattgtaa tggagatgca gcagcagacc agttgataga tgcgtttgag aaagttttaa
     6241 atttaaaaga gcaaagtagt gaaaatcata taagaccagt tatgatacat gctcaaacag
     6301 taagagaaga ccagattgat gatatgaaag taataaatat gataccttca tactttgtag
     6361 ctcacacata ttattggggt gatattcata tcaaaaacct tggagaagat agagcgttta
     6421 agattagccc acttaaaaca actatagaaa agggattaat ttacactctt catcaggata
     6481 caccagtaat agcacctaat atgctggaaa cagtatggtg tgctgtcaat agaattacaa
     6541 aaaaaggtat acaaatagga gaaaatgaaa agataagccc attagatgct ttaaagggtg
     6601 ttactataaa tgcagcatat caatattttg aagaagataa aaaaggttca attaaagaag
     6661 ggaaattagc taatttgata atcttagatg aaaacccact tacaatcgac cctatgaaaa
     6721 taaaagatat taaagtattg cagactataa gagaaggaga agtattgtat tctttgaagt
     6781 agatgaaagt tagatatttt ataatctata aaaaatagtt ttatataata agttaggtag
     6841 aattaaataa gattacagtt aaatcaacaa ttatattttt attaaatttt aattattgta
     6901 ttgaagattt ataagtataa ttttattgat tct
//
LOCUS       DAYAOY010000152         6912 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYAOY010000152 DAYAOY010000000
VERSION     DAYAOY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.156"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(533..1747)
                     /locus_tag="ACGI6B_003727"
     CDS             complement(533..1747)
                     /locus_tag="ACGI6B_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFG9753134.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            2173..2889
                     /locus_tag="ACGI6B_003728"
     CDS             2173..2889
                     /locus_tag="ACGI6B_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9753135.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            3024..3650
                     /locus_tag="ACGI6B_003729"
     CDS             3024..3650
                     /locus_tag="ACGI6B_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9753136.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(3850..5394)
                     /locus_tag="ACGI6B_003730"
     CDS             complement(3850..5394)
                     /locus_tag="ACGI6B_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9753137.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(5420..6061)
                     /locus_tag="ACGI6B_003731"
     CDS             complement(5420..6061)
                     /locus_tag="ACGI6B_003731"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HFG9753138.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            6331..6525
                     /locus_tag="ACGI6B_003732"
     CDS             6331..6525
                     /locus_tag="ACGI6B_003732"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9753139.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFD"
BASE COUNT         2537 a          884 c         1001 g         2490 t
ORIGIN      
        1 aaattcaatt aaaataaata actctttata aaaagcaatt atttaggtag aggaatattt
       61 gctaacttta atagtctttg ttaactttaa ttttatttaa gttattatag tcttactaat
      121 tttataattg tagatttaac tactttatgt tcttttattg tttatgtagt tgatttagaa
      181 gttattttaa ccatttattt taattgaagt tgtttgactt ttaaatcctt tgtgtaatct
      241 ttgtttttct agccatgata aaagtatttc tatttgtatc actatatata ataatatcat
      301 gacaaaatat ttattgtcta actaattata atctatccaa aatgaaaatg ggtgtgagat
      361 attattacaa caaattgtat tagtattaaa aagattaaaa aattaaaaaa ctaaacagtt
      421 aaataattaa acgattaagt gactgtcata taacaattta taattttatt taacagccac
      481 ttaggtattt aagagcgata gaattgagtg tatatcaaat tataataatt tcctagtaaa
      541 taattgaaaa ataaggtagt ccatttttat atttgatatc tggtttacct tgaataagtg
      601 gatatagata atttagggct tcatcagtta tataattaca ttcttcattt atccactctt
      661 taggaaaata ttttgtcttg ttagctattt tagaaatatc agtatgacta acttcatact
      721 tataaggatt attgcatgtt ctattaatga tagccataac tcctgtatat ccttttttag
      781 atagttctat cccatatgca ccaacttcag ctgcttgttt taaatcagta tctgaagcta
      841 aatgcatagc acatctttgt attacaccaa gttctaaggt ttttactctt gttgtaattg
      901 atgatgatac tataagattc tttagatagt tgcttactcc acctaattgg gaatgaccaa
      961 atttatcatg agcagaagtt ttttgttcgc atataaagtt gccgtattta tcccttatac
     1021 cttctgatgc aactatataa gccttatttt tttctttaaa tatttttcta acatcttcta
     1081 taaacttatc ttcatagaaa tcaatttctg gaaggtatat taaatcaaca agtggagtat
     1141 cattaagtct agctagtgaa gaagaagctg ctaaccatcc agtatctcta cccatagttt
     1201 ctactataaa tataccatta tttttgtata tagaaaaatc taagtatgtt tccattatta
     1261 gtgtagaaat aagctttgca gcagaaccaa atccaggaca gttgtcagtt cctaataaat
     1321 cattatctat tgttttagga atacctataa attttatttt acaagtagtg tttttgcagt
     1381 attgagaaaa tttcatcact gtgtccatag aatcatttcc gcctatataa aataatgtat
     1441 ctattttata ttcatctagt atatttataa ctctatcata atcataagaa tttattttat
     1501 aatcagccag tttgtatcta caagaaccaa gaccagacga aggtgtttgc tttaaattta
     1561 atacattatc tttgtttaag gagtctgaat ctattatatt tttgtttaat ataccttcaa
     1621 tcccattgat accaaaatat actttatcaa agtatttagt ttctaaattt ttttcataaa
     1681 taccaactgc agaagcattg ataacagctg tagggcctcc agattgggca ataatacagt
     1741 ttctcatttt acatctccct ttcattaata ttgttatgaa tggtactttt tatcgagtaa
     1801 atttatattt atagtctgat tatatattaa atgaactagc tatatcaatt gagttagtat
     1861 agacaataaa tgccataaga atgtggtaaa aaactaactt tttatataca attaatgtct
     1921 ataatttacc acaatataaa ggtactttta aggagtagtc aaagtgttgt gaaaaaaaat
     1981 atatcagtgt aatattaatt tacaaagcaa aataagttca cagttaacaa aagcagaata
     2041 agttcataaa agaaatatat acagcaataa aagtaatgct aaataaaagt aatactggat
     2101 taaaaaatat taaaaaaata ttaaaaacta atatttaata ctaattaaag taaaatatgg
     2161 aggtatacaa atatgaaaca cataaaaata gcagcaggat tagcacatgt aaattacggt
     2221 catatatcag ccgtagttaa agaagcaatg gatgcaggtg tagactacgt acattcagat
     2281 gcagcagata tgcatgactt aaaaaatatg caacttatgg gtggtcatca aataatagaa
     2341 gcaataagac cggtgacaga taaaccaata gaatgccata tatacacaag agattgtgat
     2401 agattattta tagaaaaaat agcagcagca ggatgtaact tgttgataat accagcagaa
     2461 cattttttag gtgctcctct tgcatacata ataaattact gtagagaatt tggtatgaaa
     2521 gtaggattaa caattggatg ttacacacca ctatgttttg ttgatgaagc tatatatgat
     2581 atagatagat tgcaaatagt cgttcatggt gttgatgaaa ctgatggaaa agataactgg
     2641 ggatggagaa agagtgctgt tgatttagta aaaagagcta gaaaaatgat agacgaaaag
     2701 aatcctaagt gtgaattagc aatagatggt ggactaagag cagacaatat ggagccatta
     2761 atagaatgta atccagacgt tgtaatatta tcttcagcaa tattcaagga taaagatggt
     2821 ataacagcag gtgttaagaa ttgtagaaaa gctatagatg aagcagctac aaagtttggt
     2881 ttagaataag ataataaact atgataaaag agatgctttc ttaatgtttt aaggaagcat
     2941 taattttatg aaaaactaca ggaagctaga ctaatttaat tgatttttta tactgaaaca
     3001 atataatcat aggaggttat taaatgaaaa aagaaatgat aaaagattat ttactgataa
     3061 caatatcagc atttttaatg gcattagcag ttaatttttt ctttgcagaa catacattag
     3121 ctccaggagg gataacagga ttatcagtcg tacttagcac attcttatca atgcctgttg
     3181 agaatatatc attaggaata tctatgccac ttttagtaat gggattatta tttttaggga
     3241 aaggttttgg aataaaaaca ttatatataa ctcttatggg cccattattc ttaaaagtaa
     3301 taccacaaac tcacataact gacaatctat tagtagcagg agtgataggt gggttattgg
     3361 taggaactgc tatagggata gcaataataa ggggctgtag tactggtgga actgatttag
     3421 ttgcaatgct tattaataag gtaattaagt ttctgaagtt gccagttata ttatttatgc
     3481 tagatggatt tataatagta gcgtctggaa taataagtaa gaattttatg atatctatat
     3541 attcattgat atctttatta ctcataataa aaacaatagg atttgtaact acaaagtttg
     3601 gtaagaatac tgaagaaatt aataatataa ataatgcttg tgctcaataa aaataataaa
     3661 atttacttta aatataaaag tttaaaaaat ataaaagctt aaataaaaag attaaatata
     3721 aaaaatgaag gtgtctcaaa atgaattttt tagttcatga ggcacttttt ctatgaaatc
     3781 aaatatattt ttatcatttg gacaatgaaa aataggttta tctctatgtt gagacaagcc
     3841 tatttttgtt tatttataag ataatatttt atttttttca gaatatagtt tttttataat
     3901 tgtactaagt ctagaaatat cattcttact taaaagagca tttatattta tcagttcagc
     3961 attatgttca aagtctacag gagcagtggt tagtattaag tccacatcat caaagttcgt
     4021 aaatgaaata gaagatagag gtatagattt aactatatta atctgattaa attcaaaact
     4081 taacttttgg actaataaat tagatacacc atcactacag tgacatatta aaatagtatt
     4141 taggggagat ttgcttctat ctattgctga tattaaatat aaagcaagat atcctatttc
     4201 gtcatcattg acttcataat tcatcaactg tttgataaca gtattagact ctttagcagc
     4261 cttataagtg taaggaaatg ttctctttat atcatttaat atgggatttg taatagtaaa
     4321 cccatagttt attctgtgta gaactgattt aaggtgatta gcaagagact ttattaatat
     4381 taaatcatcc tttaagttta aatttagtat acttccccaa tgaacagcta ttgcttcaga
     4441 tagattatac tctgtaattt cttcattttt aagaggttca agagtagtag ttttctttat
     4501 tgatttaagt aatccttcta tatgaagaag gatataacag acctcttcat caataaattc
     4561 tacgtcaaaa gcttcactta ggttagcaca taaaattttt gatttttcaa agtattcatg
     4621 ttctaataat tctctaaaaa aaccttcaga taagttaata tatcttttaa ttgaaattct
     4681 tttaatacat atagctatat gtattaagaa gtttatataa tactcatcac taaggttact
     4741 tttttctact tgtaaggtgt gtagagtgat ttgagataat ttctcaaaat catagttaaa
     4801 tagctcctta aatatttttg ttgatggggt atctttgcat tcattagatt tcgagaataa
     4861 tatttcttta agttcagtat aacttttatt aaccgtagca agttcaaaaa ttgcttttcg
     4921 aatatctttc tcttttccct ctaaatatac tccattattg gtttttctaa ctagagtcac
     4981 tttaaagttt tgcaaaaaat tttgaataac taccaagtct ttatgcacag tagctctact
     5041 aacatgtagt tcattgcaaa tatcacgaat cctaaactta gaatttttaa taaataattt
     5101 acttagaata tacctttttc tatcttcagg agagtatgca tatatacaat ttgatttatc
     5161 attgatatct ctaataactt taagttttat atcttcatta cctagaataa taacccctga
     5221 accagttttt ttatctaggg ataaattaaa ctctaaaagc cattcatcta aaataacaag
     5281 gtcattacga atagttttat tactaacttt taattcttcg gcaatagtat ctacagtaat
     5341 atagtctgag ctgttgagta ataattcagc aatatctaat aatcttttgt tcaatgcaaa
     5401 ccctcctaaa tcttagttac tatttattaa ccttggacca atctctttca aaagtattgg
     5461 caccatctat tgtaagctgg ttaaaagaag cttttttaaa taaatcatat gttacagctg
     5521 cataatcaca tccacaaaga gatgcttgag ttaagtggaa tacattttta atagaagctg
     5581 caacaacttt agtattatat ccatttattg atataaggtt acatatttct gtaaggtttt
     5641 ttataccatc attcccattt tcatcatttc tacctataaa agggaatagg taatcagcac
     5701 ctgcatttat agctaatgat gcttgattta cattgaaaat caaggtacat gcagttttta
     5761 cacctttact atttaatatg tttataagtt ctaagccaac ttcagagaaa tttattttta
     5821 ttatagtgtt tttatttata tttaatatct tatcacatga attaattaat tcgtataaat
     5881 tatttccaat gacttcagca gttaatattt ctggatttaa attagcacat gtctcaataa
     5941 attcatggaa gtttactttc tctttaagat acatagatgg attagcggtt attcccttaa
     6001 atccatactt tagacacttt tgaatttcat caagattagc actgtctatc aaatatttca
     6061 taatttaatt ctcctttaag ctatttgtca taaaaaagtg tatatactct atttttatta
     6121 ttaagtcaat tatacaataa gattgattat aaaattataa catgggtact taataaaatt
     6181 tacataatga tatggtaata ataaaacatt gattgtctaa taattaccat aagctttagt
     6241 aaataagagt taacagtgaa ggtattgcct taaaaatcct atgatatgta taatttgaat
     6301 atgaaggcaa taatttaagg gaggaaaaat atgattaact tttatcaacc atcaagaata
     6361 aattttggac aaggaacttt aagtgaatta cctagaataa taggaaaata tggaaagaaa
     6421 tgtttgttag ttactactcc aaatgtagag ccattagaca agttatataa aagagttaca
     6481 ttgaatttaa tagaaaaagg tatagaagtt gtacactttg attaaaaaat agctacgcac
     6541 actcgtgact ttagtcatga gttaatagct aaaatagtca gcatataggg aaacctatat
     6601 gtagagatag gatagaacct atccaatact acttgaattg ctggaaaccc ctaaagctaa
     6661 ccaaactaaa acataaggat gaaataaacc taagtgtgaa agttacgaaa gtagaaaaaa
     6721 ttggttagat ggtataaggt taaatcctaa gtactttaaa atgggcaatc agcaggtaag
     6781 ctccgaatag gagaaacttc aacgactatt cctcttgagg gaagtacact acaagttatc
     6841 ggtagtggaa gtgggtagac cttaacggat aatgccgaag gataagatat agtctgtgct
     6901 tgcatgaaag tg
//
LOCUS       DAYAOY010000153         6854 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYAOY010000153 DAYAOY010000000
VERSION     DAYAOY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.157"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            139..1038
                     /locus_tag="ACGI6B_003733"
     CDS             139..1038
                     /locus_tag="ACGI6B_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9753140.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            1247..1396
                     /locus_tag="ACGI6B_003734"
     CDS             1247..1396
                     /locus_tag="ACGI6B_003734"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9753141.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(1656..4223)
                     /locus_tag="ACGI6B_003735"
     CDS             complement(1656..4223)
                     /locus_tag="ACGI6B_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HFG9753142.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4226..4831)
                     /locus_tag="ACGI6B_003736"
     CDS             complement(4226..4831)
                     /locus_tag="ACGI6B_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9753143.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(5205..6665)
                     /locus_tag="ACGI6B_003737"
     CDS             complement(5205..6665)
                     /locus_tag="ACGI6B_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFG9753144.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
BASE COUNT         2157 a         1308 c         1017 g         2372 t
ORIGIN      
        1 ataaattgga caatttttat gttgatttta ttcatacaaa ttaaagtaac ttataaaatc
       61 tccacaataa aaaacatttt ttattcacta aactgctcca tcattggttt agcaataaat
      121 atatttttta ggtctttaat gtcaatagtg ttaaatatgc cagcatattt atttgatagt
      181 aattctattg caaatattta cagagtattt cctatatcat ttagctttat agttacagga
      241 ctatatttca gttataaaaa taataaaaat aataagatta actttgaggc agttttagag
      301 gacaaaaaaa acttaatttt cttattgagc cttatgtttg gaatgtactt gtatctatgt
      361 ttgagtttgt caatatacta tactgttgga aatgtattga ttttaaaatt atatggaatc
      421 aagtctgcca taatagtttt ggtaggtaac tatattgcag aaagatatag ctttagaatt
      481 actcaaataa aaggttatga tattaaaaat caaatagaat atgtagaaag tgtaaagaca
      541 gaatatgaga ttaataaatt gaatgatatt gtatatacag atttactgac aggtttctac
      601 aatagacctt ttgtgaatca aaaactatca gaatggacat caaatcacta caaatttacc
      661 ttatgtttta ctgatttaaa tagcttaaaa cctgtaaatg ataaatttgg acaccaattt
      721 ggtgataaat acatatccac tactgctcaa ataataaaaa aacttataaa tgtagaaaat
      781 tccttactat ttagatatgg tggagatgaa tttttattcc ttttacagaa tacaagttgt
      841 gtagaagcag aaaagataat gattaatata aattatgaat tatcaaaatt aagtaatact
      901 gacgagtatc cttatgctct ttccataagc tatggattag ttgagtggga tggaatatcc
      961 gatattgaaa ctttaatatc aaaggcagat tctttgatgt atgaaaataa gcttaaaaat
     1021 aaatctactt taaactagct agttaagata ttctacaaaa tatatgttat aagagagtac
     1081 taagaatatc aaaatatata gattttgcaa atattgaata cagtttacta atgaatgcac
     1141 tggatgtaag tgttagtaaa cacctattag atgaacactt tactgttgtt tgggctaatg
     1201 accgttatta tgaaatgttc agctatacct agctaaaaag ttaaaaatga ccactgtagc
     1261 cgagggaata gagaccagtt ttcaaaaaga tttcttgcaa gaaataaact gtgatatgtt
     1321 acaaggatac gtgttctcta gacctttacc aataaaagac tttgaaaaac ttatgtttca
     1381 aaactcctcc aattaacaat ataaaattgc ttattataag tgatattatt gtatcaacaa
     1441 ataaaggaat tgtctcaaaa ttgatttttt ctaatcaaat ttttattttg agacattctc
     1501 atttttttgc ataagaaaat acccaccaag ctacggtttt tcttaccgct gttcggtggg
     1561 tattagtagt attatgaaat ctgaaaactt taagtattac tacttccgct aacgcaatca
     1621 caaaggtcga aagcttcaaa gcgaaaatag gctttttact catcaaacgt caccgtacct
     1681 gccaacccgt ccaccgttat catttgtccg ctcttaatag ttcgtgtggc aatgtgtgta
     1741 gccataacgg ctgggatgcc atattcacgt gcgacaatgc ttgaatggct aagaggacca
     1801 ccaatgtccg ttacaatcgc acttgcagat gaaaataatg gtgtccatgc tggagttgtg
     1861 gtaactgcaa ccaaaacact tccaggttga aagctttcaa aatctgctgg actattcagt
     1921 acacaagctg gagccgtcac aacaccagta ctggtaccaa tgcccgtcaa cacaattttc
     1981 ccatctttct gcttctgttg tgcttgtgat atagtttttt tgtttttctc tggcaacttg
     2041 gatggtgaca tataacccat gtatttttta agttcagctt ttcgaacagg tattatcccg
     2101 ttcatatcag atagagactt gtttttatcc aactgctcta taagtacctc caattccgat
     2161 tttgtgagcc aataaatatc atctaaatgt gaaatagctc ctcctcgtat gaatcgttct
     2221 gagatttcct gaaacataca acgaatcaga ggatgtccca ttcccatcaa ataaatagca
     2281 ttttcacgca taggagaggt ttcctgtgcc caatggagca acttaaaaaa tagtttttta
     2341 cgcaatccac ctatgtgttg taaaatctct tcttctgcat gttttctacg cttttcaaac
     2401 gtggtctggc gtaaaaaagg actttcccct tttccttcca caaatgtttt tacggattca
     2461 aaagttggtg taagtgtttc ctgcggtgtg gaatgcgcaa aatcaaactc ataagcggta
     2521 cgaccaaact ctttaaagta cttattgatt cgatttttcc attctatcca tacttcctgt
     2581 gaaactccaa caggcacaac tgaagacatg aaatcttctg ctatctttat tgcaggactg
     2641 ttttgcagat aaaggttgat gctgttattt tgctttaccc attctgaaat accccacaac
     2701 tccttttccg attgcagaac aatcgtatca aacccaagca ggaaaacgga agtatccgtt
     2761 ataccagcac gtcgaactgc tccttgaaat aattttgtaa ataatgtttc actgatagaa
     2821 gcagcaggca atgtaagctg aatccttgtg aaataaatac atgcggcata aaatattgtc
     2881 tgaataccct ccataatttg atgggcgttc atagtatgca ggggcttttc ttcccattgt
     2941 ttaatagcat cctcatatcc ttttcgtgcc gtttcccagc gtgcaacgct gttcataagt
     3001 gtcctacgta aagagcgtgg ggaaagggat ttgatagcaa tcaaaagtgg ctttgattta
     3061 gcagaaagat aaacatatcc attaataacc gtatatgccc cgttttttgg taataatttt
     3121 ttcgcacttt taccaaacat atctatccat aaaagctctg atgcacggtt tacgatttca
     3181 agaccaagtg tggcgaacaa aggtgtaaca ggatttggca aatgttctgc caaacttccc
     3241 tttgtgtata caacatcctg ttctggcaat acccattctg gtggcagaac agtaattgga
     3301 cgagcctgaa caatgtacaa tttatctttt tccagtgccc actctacatc cataggcatt
     3361 tgataatact tctcaatttt ctttccaagc tgtatcagtt gcattacctg attacgagta
     3421 agagcatatt tttttctcaa cctttcagga accatggttt cttctgttcc ttctgaagta
     3481 cgaaccgtca taatctcttt atttgccact tcatacgata taattcgttc ggagtcctta
     3541 tccactacga ttgtatcggg tgtaactaag ctagaaacca ccgcttcacc aagcccccat
     3601 gcagcgttaa taatcatttc gctacgtcta ccattgattg ggtttaacgt aaacataata
     3661 ccagatgaat cggaaaatgc aagcttttgt actacaacag caagtgcgac aatctcgtgc
     3721 tttatattat tcttcacacg ataagcaata gcacgagctg tccataagga agcccagcat
     3781 ctctttacag agtcaataac tttatcttca ccttgtatgt taagataagt ttcctgctga
     3841 cctgcaaaag aagcgtcagg taaatcttca gcagtcgcag acgaacggac agccaccgat
     3901 atgttcccta atccagcata agccatttta attgcgtccg acacttcctg tggcatttct
     3961 ccattatgga aaagctcccc tattttctta gatacatctt caagttggct ggtattattg
     4021 gaatcaatac catctagcag tttattaatt cgagactgaa tatggttcgt ctcaacaaaa
     4081 atctggtatg aagcagtcgt aacatgaaaa ccgtctggca cagggatgcc tgctgtcaat
     4141 agttttgata aagacatccc ttttccacca acaatttcaa tggttgcttg tttatgttcc
     4201 agtggcaaga cataataaat cataatcatt cacctccatc attcataatt ccgttcagaa
     4261 atatattcat tgtttcatcc aacatttttt ccgaactatc caatggtcgt gacgtgtagc
     4321 tgattgataa catggagtta attaacaaac gtgtcacaag agcggtatcc gttttgcgaa
     4381 agatgcctgt ggtcacacca ttatcaatta ccgattttat aatatcttga tatgcataac
     4441 gagcatcttt aagtctcttt cgatactctt cttctaaata atcagattct gtatttaacc
     4501 acatcagtac agttctatac tgctctggaa atctaagatg attaagcatg atttttctaa
     4561 ggcactgttg tggtgaaggt tcaccatcaa taatcctatg aacttgttgg atagtttttt
     4621 ctatttcttt ctcaactgca tatacaacaa tctcgtcttt cgttttaaaa aaatcgtata
     4681 gacttgactt tcccatgttg gctaaaactg caatttctct cattgatgtt ttttggaatc
     4741 cattttcagc catgagactc aaagcaacag caactatttt ttctcggcgt tctgcttgtt
     4801 gttcgggtgt aagcacaatt ccttttggca tagtgcgata cctcctttta gcgaccactg
     4861 gtcgtttctt ttttatttat catacagcaa ccaacggtca cttgtcaagg ttaatttttt
     4921 tcattgtata tggtactcga gataaaaatg ttcattttag aaattactct atttaagtag
     4981 aaataggctt catcattaga aagcaagact ataaatttga gatatttatc atgttcaaat
     5041 ttcatcaata tcaaagttta aaatattaat aaaaaaagaa ttactaagaa ataacatata
     5101 aagattcaaa aaaggagttg aacttaaaat tcaattacaa aaaatcaaat ttaagacaac
     5161 tcctttattc tttatctcta ctattcttta tctctattta tttattacaa ctccctaaca
     5221 tttaccacta ctgccttagg tctagtcata gcctcaattg aatacttaac accttgagtt
     5281 cccataccag atgcctttac acctaaaaat gggaagtggt ctggacctct ttcagtctta
     5341 ttatttattt gaacagtacc aacttcaagt ttatcagcta tataaaatgc cttatcaata
     5401 tcacttgtaa atactgatga ttgaagccca tactctgatt gattagcaat ttcaattgct
     5461 tgatttatat ctttaactct tataattgga agtactggac caaatggttc ttcccaagca
     5521 attttcatat caacatttac attatctaac aatgttggat agaataaatt attttttcta
     5581 acattgcctg ttattaactt tgctccttta tgtaatgcat catcaaccaa cccttgaaca
     5641 aaatcagttg ctttattatc tataagagga gtgataacta cttcatccat tggatttcca
     5701 attgtcaact tctctaccaa tggctttatt tttccgacta atttatccgc cacactctct
     5761 tgtactaaaa ttctctttac tgccgtacat ctttgtcctg aatatgaata cgctccagat
     5821 actatattct tagctgcaaa atctaagtct gcatcttcta atactatagc cgcatctttt
     5881 ccaccaagct ccaataataa tggtaccatt ccagatattt tagatatatg ctgaccaact
     5941 tcagtactac cagtaaagtt aataaaatta attcctttat gcgttacaac ataatcccct
     6001 atctcactac ccctacctgt aatagtatta agtactccat ctggtatccc agcttttctc
     6061 ataatttcta ccacatgaag tgcgctaata gccccttgtg tagcaggttt taatacgact
     6121 gcatttccac caataagtgc tggagcaatc tttgacattg ataaatttac aggataatta
     6181 aaaggagata tagctaaaac agtacctaat ggaacccttg aaacataaga catcttattt
     6241 cttgttccac cagggaaatt atctccacta atggcttctc cctccatatt ttttcccaca
     6301 tcagctgtgt atcttaaaaa atctgctgtt ctctcaactt ctgcccttgc agattttatg
     6361 tctttggcta tttcaagtac cagtatatta gctatctcat ctatattttc taacaataaa
     6421 tcagcagcct tatgaaatat attgcctctc ttgtatacag gcatttcagc ccatactttt
     6481 atactctctt ttgtgttttt tataacctca tctacctcat gttttgtcat agattgtact
     6541 ttcccaacaa gagaattatc tattggtgag taaatttcta ttggctttct cgaattagat
     6601 tctacccatt ttccatttat taaattttta tatacatttt cttttgtttt aagttcatta
     6661 aacatatttt tattcctctc tctgatataa ctttgtgtat ttaaattata gccatttatt
     6721 cgttatgtaa acatttcttt ttttcataaa cgtttaccta taaaaaaatt ttaagttaac
     6781 gtttttctac attcttctat tttaataaca tactcgctcc aaatctatat aggtcacact
     6841 ttaatactaa tata
//
LOCUS       DAYAOY010000154         6853 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYAOY010000154 DAYAOY010000000
VERSION     DAYAOY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.158"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..931
                     /locus_tag="ACGI6B_003738"
     CDS             <1..931
                     /locus_tag="ACGI6B_003738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753145.1"
                     /translation="KKIQNELPRFLQSKGFNIKRGHEHSKARHMQPEDYKKQQIQELN
                     ELENKLEYVKKLFKGFEKPLNSFDELEPANVKKSLITGKETVYKNDYDEVIDILKGTM
                     KENLELKEKLHNEQLKTRNLEVKLSTVNEYERLTYEKEKKLKLEKEEFEEQKQLISER
                     EKNIEKVLENKDKNNKKILDEKEIEINELEKQYKNLSRKYNNTIREYEKVIEEFSNND
                     IYIIFNKSSIKEIQENDTYRFKDANSLIKVLNERYFNSNETKEIGFNMSFGANNENVF
                     LQKNIILGQYIDIDLRQKIIISNRKHTLISRDI"
     gene            1039..1407
                     /locus_tag="ACGI6B_003739"
     CDS             1039..1407
                     /locus_tag="ACGI6B_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753146.1"
                     /translation="MKKIPKTEHMVMNFIWSQNKSKVSSKDIANYMAEEYNWFKGTTG
                     KVLSRLSDKGFLEPEKEGRNTIYNVKINRDEYIKFETEEFFSFVHHNSIHSFISALGE
                     GEDISDEDIKELEDWIKNRG"
     gene            1415..2677
                     /locus_tag="ACGI6B_003740"
     CDS             1415..2677
                     /locus_tag="ACGI6B_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9753147.1"
                     /translation="MNIFIEIIKLNMYMSLPIILFILFNKKLLVKYTAMFSYTLCLGI
                     ILRMMIVSKVNLNIPIIDNENIALSRKSYIFSDYIFENNFLKILFYIWIIGVVLISLY
                     YIYCHIKFCNYITNLSKEILDTSIYNIFNDQKENLNIKKKFKLYVVEGIYSPAITGLR
                     KNKIIIPNKKFKEKELKLIFRHELIHYKRKDNFLKLIMLIVNILYWFNPICYLLKKHF
                     NELCELSCDELVIKGYNIDEVKDYAYVLLDTIKYKNKLKQSICISQFYTEKNSIIKRR
                     LDRILNVKSQKRGLNLGLLISLVIIGSIFTFNLNINAQNIIISNNENGNIRYNKNGET
                     YGTSIINKDGDIIEEPNLIYAQGENNVKGYVKKNDLYDEENQPQNPKEAVAYMKEKRK
                     LSLNPFYKNTIPIYDKDGITILGEFKID"
     gene            complement(2773..3531)
                     /locus_tag="ACGI6B_003741"
     CDS             complement(2773..3531)
                     /locus_tag="ACGI6B_003741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753148.1"
                     /translation="MIKKSKLSKALAISLAAASLFSNNAFADTNRPNLNIKEYSNVSM
                     DRKSTSNTTSFSANGKNYYNYTSVEGTYAGAYGSTVIKCTSGKVTDGIMGVHANLYNS
                     SGNVVKTTNWQYNTRTTDFHSASTKMHNVKGTYHTQGTTKAYNGSGYKTVGAKKSPIA
                     TVKSLNIDISEQELKERQYLYETKNLISAVGFNDIKGYVSLDDLYDEENQPQNPKEAV
                     AYMQRLERSNIKYKSIPLYDKDGETIIGEYHIDL"
     gene            complement(3893..4486)
                     /locus_tag="ACGI6B_003742"
     CDS             complement(3893..4486)
                     /locus_tag="ACGI6B_003742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753149.1"
                     /translation="MNKDKFNELDISEQIEYINNQLENNETLTYICKNIGIGRSTIRD
                     RFKKANYSYSKDLNKYIHECITDVLQDPSIKNNRCITTDIQKEKNNISNTNVIQSDAV
                     KEVINKSDKEIKDNLLDIVSNYDILKEIIELHKCNTSVIKQQIVIDLEDSDSKLTTLR
                     VNSKELELFNKFCDNNKQFKKMDLISQALKEFREKYK"
     gene            5504..5860
                     /locus_tag="ACGI6B_003743"
     CDS             5504..5860
                     /locus_tag="ACGI6B_003743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753150.1"
                     /translation="MKDYLIENTTKEQRREFVAGALGIASIDSHEPSEIILKLSDRYI
                     EGELEIEEIRDIVLSFYKRRYLDGDESIETLEDYVLILEKEKRRNNIVTDEEGIEKIM
                     DKIIDENLDALKKLSE"
     gene            5875..6099
                     /locus_tag="ACGI6B_003744"
     CDS             5875..6099
                     /locus_tag="ACGI6B_003744"
                     /inference="COORDINATES: protein motif:HMM:NF042874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6718 family protein"
                     /protein_id="HFG9753151.1"
                     /translation="MYIVAKKFGEVGCIASKVERGERVGALIEYLSLTMLDKGIEIVV
                     LDKPNMYGEYAPYEFLSEVEFVCSVLSMNH"
     gene            6384..>6853
                     /gene="mobV"
                     /locus_tag="ACGI6B_003745"
     CDS             6384..>6853
                     /gene="mobV"
                     /locus_tag="ACGI6B_003745"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFG9753152.1"
                     /translation="MAFLVCHVAKYSRNNIVGIQKHNQRQNKSYKNKDIDITKSNKNI
                     DFENKTRIKYLEKVEKIIKENRTKGKVIRKDAILMCENVISASPEFFLGMEQSEKEKY
                     FKYAYEFLKDRYGDKNIVSATVHFDETTPHMHFNFVPITSDGKLSARDLITRIEL"
BASE COUNT         2721 a          713 c         1041 g         2378 t
ORIGIN      
        1 aaaaaaaata caaaatgaat taccaagatt cttacaatca aaaggattta atataaaaag
       61 aggacatgag catagcaaag caagacacat gcaaccagaa gattataaaa aacaacaaat
      121 tcaagaactt aacgaattag aaaataaatt agagtatgta aaaaaactat ttaaaggctt
      181 tgagaagcct ttaaatagtt ttgatgagtt agaacctgct aatgtcaaaa aaagccttat
      241 aacaggcaaa gaaacggtgt acaagaatga ttatgatgaa gtaatagata tactaaaagg
      301 aacaatgaaa gaaaatctag aacttaaaga aaaattacat aatgagcagc tgaaaacaag
      361 aaatttagaa gttaagttaa gtacagtaaa tgaatatgaa agattgacct atgaaaaaga
      421 aaaaaaatta aaattggaaa aagaagaatt tgaagaacaa aaacaactaa tatctgaacg
      481 tgaaaaaaat attgagaaag tactagaaaa caaagataaa aataataaga agattcttga
      541 tgaaaaggaa atagagataa atgagttaga aaaacaatat aaaaatttat caagaaaata
      601 caataacaca ataagagaat atgaaaaagt gattgaagaa tttagtaaca atgatatata
      661 tattattttt aataagtcta gtatcaaaga gatacaagaa aatgatacat ataggtttaa
      721 agatgccaat agtctgatta aggtattaaa tgaacgttat ttcaatagta atgaaacaaa
      781 agaaatagga tttaatatgt catttggagc taataacgaa aatgtctttt tacaaaaaaa
      841 tattattctt ggacaatata tagatataga tttaagacag aagattatta tttctaatag
      901 aaaacataca ttaatatcta gagatatttg aaaaaaattt agactactta taaactctat
      961 ttaaataaaa gaaattaaaa aaattgactg ttagacaaat gtctaatata ataaaaataa
     1021 aggttggtga tttgtattat gaaaaaaata cctaaaactg aacatatggt aatgaatttt
     1081 atttggagtc agaacaaatc taaagtaagt tcaaaggata tagcaaatta tatggctgaa
     1141 gaatataatt ggttcaaagg tacgacagga aaagttcttt caagattaag tgataaaggt
     1201 tttttagaac cagaaaaaga aggtagaaat actatttata atgtgaaaat taatagagat
     1261 gaatatataa aatttgaaac tgaagaattt tttagttttg ttcatcataa ttctatacat
     1321 agttttatat cagctcttgg ggaaggtgaa gatatctcag atgaagatat aaaagaactt
     1381 gaagattgga taaaaaatag gggatgatgt aagtatgaat atatttattg aaataataaa
     1441 attaaatatg tatatgagtt tacctattat tttatttatt ttatttaata agaaattatt
     1501 agttaaatat acagctatgt tttcatatac tttatgtcta ggaataattt taagaatgat
     1561 gattgttagt aaagtaaatt taaacatacc tattattgat aatgagaata tagctttaag
     1621 tagaaaaagt tatatcttta gtgattatat atttgaaaat aattttttga aaatattatt
     1681 ttatatatgg ataataggag ttgttttgat atcactatac tatatctatt gccatataaa
     1741 attttgtaat tatataacaa atttaagtaa ggaaatatta gatactagta tatataatat
     1801 ttttaatgac caaaaagaaa atttaaatat aaaaaagaaa tttaaactat atgtggttga
     1861 aggaatctat tctcccgcta ttactggatt aagaaagaat aaaataataa ttcctaataa
     1921 aaaatttaaa gaaaaagaat taaaactaat ttttaggcat gaattaattc attataaacg
     1981 aaaagataat ttcttaaaat taattatgct tattgttaat atactgtatt ggtttaatcc
     2041 aatttgttac ttattaaaaa aacatttcaa tgagttatgt gagctatctt gtgatgaact
     2101 tgtaatcaaa ggatataata ttgatgaagt taaggattat gcatatgttt tgctagacac
     2161 aataaaatat aaaaataaac ttaaacaatc aatatgtata tcacagtttt atactgaaaa
     2221 aaatagtata attaaaaggc gcttagatag aattttaaat gttaaatctc agaaaagagg
     2281 cttaaatctg ggtttattaa tatccttggt aataatagga tctatattta catttaattt
     2341 aaatataaat gcacaaaata taattattag taataatgag aatggaaata ttagatataa
     2401 taaaaatggg gaaacttatg gaacatctat tattaacaaa gatggtgata taattgaaga
     2461 accaaatttg atatatgctc aaggcgaaaa taatgttaaa gggtatgtga agaagaatga
     2521 tttatatgat gaagaaaatc aaccacaaaa tcctaaagaa gcagttgcgt atatgaaaga
     2581 gaaaagaaaa ttaagcttaa atccatttta taaaaataca ataccaattt atgataaaga
     2641 tggtataact atattaggag aatttaaaat tgattaaaaa atgagttata atatttaaat
     2701 aaaataaaac actagtctaa ataattattt agactagtgt tttattttat ttaaattaag
     2761 ttatgtagca tattaaagat ctatatgata ttcaccaata atagtttcac catctttatc
     2821 gtaaagaggt atgcttttat atttaatatt tgatctttct aatctttgca tatatgcaac
     2881 tgcttcttta ggattttgtg gttgattttc ttcatcatat aaatcatcaa gcgatacgta
     2941 tcctttgata tcattaaagc ctacagctga aattaaattt ttagtttcat atagatattg
     3001 acgttctttt aattcttgct ctgatatatc aatatttaag cttttaacag ttgcaatagg
     3061 gcttttttta gcacctactg ttttataacc gctaccatta tatgctttag tagtaccttg
     3121 tgtatgataa gttcctttta cattgtgcat tttagttgaa gctgaatgga aatcagttgt
     3181 tctagtattg tattgccaat tagttgtttt aacaacattt ccacttgaat tatataagtt
     3241 tgcatgtact cccattattc catctgtaac ttttccagaa gtacatttta tgactgtact
     3301 cccatatgca cctgcataag taccttcaac tgaagtatag ttataataat ttttaccatt
     3361 agcagaaaat gatgttgtat tactagttga ttttctatcc atagacacat tactgtattc
     3421 tttaatattt aagttgggtc tattcgtgtc agcaaatgca ttattagaaa acaatgatgc
     3481 tgcggctaga gatattgcta gagctttgct aagtttactt ttttttatca taaataatta
     3541 ccccttaaaa attatattag acaaaagtct tatttatcaa taattgtatt agacaaaagt
     3601 cttaatgtca atatatttat agaaatattt tctatttaga aattaaaaaa taattattgg
     3661 taagtagaat gtatagtaaa aaaactaatc cttaaattga cgtttaggga ttagttggat
     3721 ttatttcagt gttttcaata cctataaaaa taaaatagta ctaacttaaa ttcattaagt
     3781 tagtactatt tttaatttct tattataatt tggataaata atttaatata ttcgattatt
     3841 aactcttatt tctatttttt actttatttt gtttgattta gcctaatgag ttttatttat
     3901 atttttctct aaactctttt aatgcttggc ttattaaatc catctttttg aattgtttat
     3961 tattatcaca gaacttatta aataactcta attctttact attaactctt agagtagtaa
     4021 gtttactgtc agaatcctct aaatctatta ctatctgttg ctttataacg gacgtattac
     4081 atttgtgtaa ctctataatt tcttttaata tatcataatt agaaactata tctaacaaat
     4141 tatcttttat ctctttgtca gacttattaa ttacttcttt tactgcatcc gattgtataa
     4201 catttgtatt actaatgtta tttttttctt tttgtatatc agtcgtaata catctgttat
     4261 tttttatgct tggatcttgt aatacatctg ttatacattc atgtatatac ttgtttaagt
     4321 ctttggaata actgtaatta gcctttttaa atctatctct tattgtactt ctgcctattc
     4381 ctatattttt acatatatat gtcaaggttt cattattttc taattgatta tttatatatt
     4441 ctatttgttc tgatatatct aattcattaa atttatcttt gttcatacga cacccccaca
     4501 gtaataacag atgtattacg tctgttattt gaaattatat cataatatta tttttaataa
     4561 atttggagtt ttaaaatata attagcaaaa atttcaaaaa atatgttgtt atatcctcag
     4621 aaaaaattta tacttaaata tataaaaact aaataaaaaa gcacttacaa aaagtaagtg
     4681 ccttctgtaa cttcagttct ttaaatcgcc taggaaacga tttaaggtaa caactggtat
     4741 agaaagtacc aagaagttaa ggaataatcc aatcgcaaga taggctaaat ctatcaagga
     4801 gagttgaccc aaccgaaatt atttacaaaa tataattata gtatataaag taaaataatt
     4861 ttagtcaagc ggaaatgttg aacacctaaa gtatagaagt ctatacttta ggtgtttttt
     4921 aatgcaataa atgaggttgt atgttaccta tatgacatat agtgggacag cccttaacca
     4981 ctcaaaaaaa aggtcaggtg agttcagcca atgcagaaag tgaacctctg tacaatatag
     5041 ctaatccctt tttatagcta gtacaggatt gagtggtgac gacaacctaa aggtcgggtg
     5101 tgggagttct aggagcataa gtgcttggga actatgaaca gaattattgc attaggatag
     5161 aaccataatg tagaaagttt ataggtgttt acacgtaata aaaagggtac agatgataag
     5221 gtgaccgaag tatacggttt atagatttgt aattacttcc ttatgatagt caatttaaaa
     5281 gtaatgacta ttataaggga ataattacgt ccaatttcag ttccctcgtg ccggtttatt
     5341 attttagtga acttgtcaag ataaaattta aataggaata acaaaattct ccatatgcca
     5401 agccccttct ggcttggcat atggagaatg cgttagtaac cacttgaagc caaaggttaa
     5461 agtggtttta gccgttaggc ttaacaaaag gaaggtggag attatgaaag attatttaat
     5521 tgaaaataca actaaagagc aaagaagaga atttgttgct ggtgcgcttg gaatagcctc
     5581 aattgattct catgaaccta gtgaaattat tttaaagtta tcggatagat atattgaagg
     5641 agaattggag atagaagaaa tccgagatat agtgcttagt ttttataaaa gaaggtatct
     5701 tgatggagat gagtctatag aaactttaga agattatgtc ttgatacttg aaaaagaaaa
     5761 aagaaggaat aatatagtga ctgatgaaga aggtattgaa aaaataatgg ataaaataat
     5821 agatgaaaat ttagatgcct tgaaaaaatt atctgagtga tatgaggtga agtaatgtac
     5881 attgttgcaa aaaaatttgg tgaagtaggt tgtattgcat caaaggtaga aagaggagaa
     5941 agagtaggtg cattgattga atacttatca ttaacaatgc ttgataaggg gattgaaatt
     6001 gttgttttag ataagccaaa tatgtatgga gaatatgctc catatgagtt tttgagcgaa
     6061 gttgagtttg tatgtagtgt tttatcaatg aaccattagt ggtctattta gaccactagg
     6121 gtgtgccaaa agtgaccact tttggtatat agggttttaa tgcctcgcag agcttaccac
     6181 tttgccaaca tagtgtaaag tatagtaagt tagacttata tgtataaata tgctattctt
     6241 gattataaat aatcaagaat ttctataaca tgactatagt catgttatag aaggcttatt
     6301 tgtatgtata agtctttttt ctattaaatt acaaaacttt aaaaatgaat attgagattt
     6361 tctaattaga agggagttta agtatggcat ttcttgtttg tcatgtagca aagtattcga
     6421 gaaataatat agttggtatt caaaaacata atcaaagaca gaataaatct tataaaaata
     6481 aagatattga tataacaaag agtaacaaaa atatagattt tgaaaataaa actagaataa
     6541 aatatttaga aaaagtagaa aaaattataa aagagaatag gacaaaggga aaagttataa
     6601 gaaaagatgc aatattaatg tgtgagaacg taatttcagc aagtccagaa ttttttttag
     6661 gtatggagca atcagaaaaa gaaaaatatt ttaaatatgc atatgagttt ttgaaagata
     6721 gatatggtga taaaaatata gtttctgcta cagtacattt tgatgaaacg acacctcaca
     6781 tgcattttaa ttttgttcca ataacatcag atgggaaact aagtgctaga gatttaataa
     6841 caagaattga act
//
LOCUS       DAYAOY010000155         6674 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYAOY010000155 DAYAOY010000000
VERSION     DAYAOY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.159"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            278..1492
                     /locus_tag="ACGI6B_003746"
     CDS             278..1492
                     /locus_tag="ACGI6B_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9753153.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(1912..2433)
                     /locus_tag="ACGI6B_003747"
     CDS             complement(1912..2433)
                     /locus_tag="ACGI6B_003747"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9753154.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            2789..3586
                     /locus_tag="ACGI6B_003748"
     CDS             2789..3586
                     /locus_tag="ACGI6B_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFG9753155.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            3696..4286
                     /locus_tag="ACGI6B_003749"
     CDS             3696..4286
                     /locus_tag="ACGI6B_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFG9753156.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4352..6049
                     /locus_tag="ACGI6B_003750"
     CDS             4352..6049
                     /locus_tag="ACGI6B_003750"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFG9753157.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6263..>6674
                     /locus_tag="ACGI6B_003751"
     CDS             6263..>6674
                     /locus_tag="ACGI6B_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFG9753158.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIM"
BASE COUNT         2682 a          663 c         1111 g         2218 t
ORIGIN      
        1 gaatttattt tatttaaaat tccttgaaag tttaaatata gtaagttgta gtaaagagaa
       61 aatataaata tttaaatgct atttaatgtg aatctattaa ttaggttata cttaaactta
      121 attaatagat tttatatttc aagacaatga gatagggttt tagattaaaa tataaccaat
      181 ggttaaatta cagacatttt gttaactttc cctcgattta ctagtaaata ttttttttat
      241 atacttaaat tacaacagat gtactaggga ggtatatatg caaattaaaa taggtaaagt
      301 catacagtgt cttagaaaag aacgaaattt aactcaagaa caattagcaa agtttatagg
      361 tgtgtcaaca ccagcagtat ctaagtggga gagtggaaat tcttacccag atatagaatt
      421 attgccttta ttagcagact tttttaatgt gtctattgat aagcttttaa attacaagat
      481 tgatttaagt gaagaagaag taatgaaaat ttataaagaa ttagaatcag gctttgctag
      541 aattgaaatt gatttatcaa cagaagaacc aaaagaagaa tttagacaag atttagaatc
      601 tgtaaaaaag ctttccaata tgtatataga gaagtatcct aaaagctatt tattgaagct
      661 aaggatatgt tcgctgtatc agatgtattc atataaattt ggtaagagtg aacttaatga
      721 cagagtaaaa gaaacaacga atattcttga agatatagtg agaaatacag atgatatcca
      781 aataaaagaa acagctctta ttatattatc aaacgcatat tgtatgttgg aggattatga
      841 aaaggcagag ttgtatttaa atatgataca taagtcaata ggggatacaa gtgttaattt
      901 agctatgata tatttaaagc aaaatagact tgaagaagca gaaatattgc ttcaaaataa
      961 attgtttagt aatgtattta atataagcat ggattgtaag ggaataatta atgtatacaa
     1021 aaatcaatac aaggaattaa agaaaaaatt ggagaataga aattttaata aaaatgcaat
     1081 tgagagagaa atggagtata taaaaaataa gttgctgggg tatgcaaatc tttctttaga
     1141 aataaaaaaa atgctcagtg aagataaagg tgcatttttt agtatgtata tggattatat
     1201 ggaattatct ctaatttttt tattttttaa catgaaagaa gaagcaaaaa aagctttata
     1261 tagtctaaaa gaaatattag aaaaatatcc tatacatgaa agtttggatg taagtcaaat
     1321 gagatttttt gataaggtag agtctaaaaa tttatatact tttaatatat atacaaactt
     1381 gttaattgta cttaatgatg acagttataa tgagcttaga gaagaaccaa tttttaagga
     1441 tgtaattgaa aagattttag atatggaaaa aatgttgaaa aataaagaat aagtaaaaat
     1501 ttaatatagg acttaaagat taatcaaatt aaaagaaagt gtttcaaaat aaaaatttga
     1561 atcacctttt ttgtcggtct taaacagtta tttagaaaaa tagaggtttc aaaatagaaa
     1621 tctgaattat ctgtattatt acacaaaaat agaaacctaa atttataaaa atagaataaa
     1681 atttgttata taaaatgtaa ttttgtctat aaaatctcac ctgttttctt aactttaagc
     1741 gtgaaaaaga ggatgtatca tgaatttttt agttcatgat gcatcctgct ttgtatgaaa
     1801 tccaatatat ctttattatt taaacaatgg aaaagagtct tatttatatt ttgagacatc
     1861 tcttttattt aattatgaag ttttatacag ttcaataaat aggaattata tttagaaact
     1921 catgacatta tttcttagat agtgattaaa tttatcatat tccattttag ataacttttc
     1981 tgggtaagac attacaatca ggttatgccc attaaatttt ttatggtatg gtggatgaat
     2041 gtgttcataa ttatttgtct tatattttac tctttcatag aacgccttgc gataaattga
     2101 aatctcatca attggaaggt caatttctaa aataatagtt tttcctttct tatttaaaag
     2161 ctctaacgct ctttgcccat atctattatt gcgaatttct ggaaaaatgc agaaatgttc
     2221 tacatagata aagttatctg tttcccaaca taaaattatg ccgacaaact ggtttttatc
     2281 ataaattaaa ttgaattgat attctttatt tttcaaaatt tctgcttgca aagaagattt
     2341 tctttgttta tgaaatggga aactaatttt atataattcc atagccttgt aatataattt
     2401 attttcactg gttcttaacc gttcaaattt cactttactc ctcctaatca cgaaaattcc
     2461 aatttattta tgagttcatt ttaccatagt cagtatttgc atacaatgtg ttttaagttg
     2521 agtgtcattt ctttctttta tttatgctta tatctcatag tctaagagat aattgcttaa
     2581 gacataacta aaaactatta tattaagctt tgataatcta aaaacagtat atttatggac
     2641 ttttctaggt ctaaaagtaa tcttaataat tttagaagtt acaaaaaagt tacataaatt
     2701 tggaaagcta taatatacat atataatata aggttaaaac tgtaatgaat aatattaatt
     2761 taaaatttga acatggaagg agattattat ggagaaaaga gaaaagagga taataatatc
     2821 atctttatta agtgtatcaa ttttaatggg tttggcaagt atatattcta tattaaataa
     2881 agaagacata agtttaaccg ttaaaggtca agagcaaaaa gtatcttcct ttaagaaaac
     2941 agttgaggaa cttttggatg aacagggtgt aaagtataat tctgaagata agattaatcc
     3001 aagtttagat acagagttaa aagatgatat gaaaataaaa gttgttaagg taactaaaag
     3061 taaaaaagaa gagattgaaa aaattccatt tgatacaaag catgtaaatg atagtaattt
     3121 gttaaaagga aaatctaaag tttatcaaga aggtcaagaa ggagaaaaaa aactagtcta
     3181 taatttaact taccatgatg gcaagttagt caaaaaagtc ttatcaaaag aagtaatatc
     3241 taaggagcca actacaaaaa ttataaaata tggaactaaa gaaaaagtac taatagcatc
     3301 aagaggagca aatataagag gaggcaaaca tatgaaagtg gttgccactg catatgcagg
     3361 agatacaata acatctactg gtacaactcc aagatggggt gttattgcag ttgacccacg
     3421 tgtaatacca tatggaacaa aagtatatat acctaaactg ggtatgactt ttgtagcaga
     3481 agattgtgga ggtgcaatta aaggtaatag aatagacatt tttatgaaca gtgaaggaaa
     3541 agcttcaaat tggggaagga aaagtataga tatatatcta cattaaatta gtgaatagaa
     3601 taactttact tgaaggacaa aaaagtataa aatatgaatt aactaaactg ttagattatt
     3661 cttaaaacat aaaaatatat ctaagggggg ataagatgaa aatagtctgc ttaggagaca
     3721 gtttaacgta tggatttgga gtatcacgaa gtaattcatg gacaaacatt gtaaataaag
     3781 agactcgatt agaaatagta aataagggta taaatggtga tacaacaagt ggtatgttag
     3841 ctaggtttaa tgaagatgtt gtaaaaaact ctcctgatat agtttttata atgggtggca
     3901 ccaatgattt tattgctggt gcgggaaatg aaattattaa ttctaatata atggctatgg
     3961 tgcatcagtc atattcaaaa aatattatac ctataatagg tataccatta aaaccagata
     4021 ttccaaatgt tagggaagat tggagttgtt ttactgattt taatatagta tctcaaaact
     4081 tagaatcata tagttattgg ataaaaaaat tttgtatgac atttaatact aatttcgtag
     4141 atttttattc agaatataat aaaaatatgg aactagaagg atataaaaaa ttatattttg
     4201 atgggttaca tccaaccaaa gagggtcata gaataatggc agacattttt attaattcaa
     4261 ttaataagta tatagaagag ttatagatta cattaaaatg cttgtttatg cgtcagttgc
     4321 aataaataca tacagtagag aggggtttat tatggattat actaaaactt atgaagagtg
     4381 gataaagggt tcatattttg atgaagatac aaagctagag ttggagaata taaaaaataa
     4441 tgaaaaagaa atagaggata gattctataa ggatttagaa tttgggactg cgggtcttag
     4501 aggaataatt gaagcaggaa cgaatagaat aaataagtac acagttagaa gagctacttt
     4561 tggattggca aactatatat tagaaaacac aacaaaagaa gaaacaagta gaggagtagt
     4621 aatagctcat gataatagac acaagtctag acaattctgt atagaatctg caaatacttt
     4681 ggcagcatgt ggtataaaag catatatatt tgatagtttg agaactactc cagaattatc
     4741 ttttgctgtt agaagcttaa atgcaatcgc tggtatagtc ataacagcaa gccataatcc
     4801 tccagaatat aatggatata aggtatactg ggaagatgga gcacaagtta tgccagagat
     4861 tgcaaatgca ataacagaga aagtaaatag tatacatgat tatagtacaa taccaacctt
     4921 aacggaagct aataaaaatt tggtagtttt gttggacgaa tcacaagata ctaaatttat
     4981 agaagcagta aaaagccaaa ttataagaaa agatttagtt aaaaatgtag gtaaaagctt
     5041 taagatagtt tatacacctc tttgtggaac agggaatgtt ccaataagaa gagcattgaa
     5101 agaagttggt tttgaaaata taattgtagt tccagaagaa gaaaatccag acccaaattt
     5161 tgcagggctt gattatccaa atccagaaga gaaaaaagct ttaaatagag gtatattact
     5221 cgcaaaagaa aaaggagcag accttgtaat agcaactgac ccagattgtg atagagtagg
     5281 tgtagcagta aaaactacta caggtgagta tgctttactt acaggaaatc aaattggtgg
     5341 tatgctgaca cattatataa tagaaggatt aaaagaaaat aataaattaa aagaaaatcc
     5401 tacaataata aaaaccatag ttacatcaga atttggagca gatatagcta aagcaaataa
     5461 tgttgatgta ttaaatgttc ttacaggttt taaatttatt ggtgaaaaaa taaaattatt
     5521 tgaacaaaat aaaaatagaa gttatgtatt tggatatgaa gaaagttatg gttacttagt
     5581 tgggactcat gctagagata aagatggtgt agtttcttca ttgttaatat ctgaaatggc
     5641 agcattttac tattcaaaag gtatgagttt atatgatggc ttaatagaac tttataaaaa
     5701 gtatggattt ttcaaagaac agactatatc tttgacttta aaaggtatag aaggtgtaga
     5761 aaagataaaa gaaataatat cttattttag agaaaatcaa attgattata taaatagtat
     5821 taaagtagtt gataaaaaag attataaaaa tggtatagat aatcttccta aatctgatgt
     5881 tttaaaatac tttttggaag atgaatcttg ggtagctata agaccatctg gaacagaacc
     5941 aaaacttaaa ttttacatag ctgtaaaagg agctagtgat attgaagcag ataaaaagat
     6001 tcaaggtttg aaaaagtata tagatgacat ggtcgaaaaa ttaaaataaa atattattat
     6061 tttataagat aattttgtaa taatatttat ttagctgaaa attataattt actttcattg
     6121 agtagaatat ggaaatgtta gtagaattgt tattttcagc ttttatttgt gttattttta
     6181 cttaaataat tatattatag aatgaatctg agtataatta atattgtata atgaaattaa
     6241 tttgatagaa aaggggatat ttatgtttgg tatagataag aaattgattg aatatataat
     6301 tgcaaaatcc aattacagtg aattaagttc aaagtcagca gaagtatctt tcttcttaat
     6361 gttgtccata tttccttttt taatatttac aataagttct atagcttata taccgattct
     6421 tcatttaaat aagtatatag ctttgtttag aaatatgatg ccagaaggtg cttttgctgt
     6481 gttatcttca atcatagttt ctgcaataga caatagaaac ctgaaatttt tagcagtcag
     6541 ttttgtactt actatgtgga cattttcaag agcagtaaaa gctcttatta aaggaatgaa
     6601 tagagcctat aaagttaaag aaactaggtc tttttttaaa attttatcta tatcattttt
     6661 atttacaata atgc
//
LOCUS       DAYAOY010000156         6584 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYAOY010000156 DAYAOY010000000
VERSION     DAYAOY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.160"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(619..1179)
                     /locus_tag="ACGI6B_003752"
     CDS             complement(619..1179)
                     /locus_tag="ACGI6B_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFG9753159.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="ACGI6B_003753"
     CDS             complement(1225..2208)
                     /locus_tag="ACGI6B_003753"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9753160.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="ACGI6B_003754"
     CDS             complement(2221..2583)
                     /locus_tag="ACGI6B_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753161.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="ACGI6B_003755"
     CDS             3826..4473
                     /locus_tag="ACGI6B_003755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753162.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /gene="repA"
                     /locus_tag="ACGI6B_003756"
     CDS             4748..6385
                     /gene="repA"
                     /locus_tag="ACGI6B_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFG9753163.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAYAOY010000157         6440 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAYAOY010000157 DAYAOY010000000
VERSION     DAYAOY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.161"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..597
                     /locus_tag="ACGI6B_003757"
     CDS             <1..597
                     /locus_tag="ACGI6B_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753164.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="ACGI6B_003758"
     CDS             728..1750
                     /locus_tag="ACGI6B_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HFG9753165.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="ACGI6B_003759"
     CDS             complement(1914..2471)
                     /locus_tag="ACGI6B_003759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9753166.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="ACGI6B_003760"
     CDS             complement(2477..3553)
                     /locus_tag="ACGI6B_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFG9753167.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="ACGI6B_003761"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="ACGI6B_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFG9753168.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="ACGI6B_003762"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="ACGI6B_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9753169.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="ACGI6B_003763"
     CDS             5391..6320
                     /locus_tag="ACGI6B_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9753170.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
BASE COUNT         2216 a         1064 c          844 g         2316 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca
//
LOCUS       DAYAOY010000158         6293 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYAOY010000158 DAYAOY010000000
VERSION     DAYAOY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.162"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..979
                     /locus_tag="ACGI6B_003764"
     CDS             <1..979
                     /locus_tag="ACGI6B_003764"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFG9753171.1"
                     /translation="NLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLLL
                     YYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1078..2040)
                     /locus_tag="ACGI6B_003765"
     CDS             complement(1078..2040)
                     /locus_tag="ACGI6B_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFG9753172.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2356..3306)
                     /gene="hpdA"
                     /locus_tag="ACGI6B_003766"
     CDS             complement(2356..3306)
                     /gene="hpdA"
                     /locus_tag="ACGI6B_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HFG9753173.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3299..3556)
                     /gene="hpdC"
                     /locus_tag="ACGI6B_003767"
     CDS             complement(3299..3556)
                     /gene="hpdC"
                     /locus_tag="ACGI6B_003767"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HFG9753174.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3560..6268)
                     /gene="hpdB"
                     /locus_tag="ACGI6B_003768"
     CDS             complement(3560..6268)
                     /gene="hpdB"
                     /locus_tag="ACGI6B_003768"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HFG9753175.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
BASE COUNT         2107 a         1134 c          689 g         2363 t
ORIGIN      
        1 gaacttaaaa ttaatatgga taaatattct aaacccagta tttattaaaa atgaaataaa
       61 aagaaaaaaa atacaaatta ttatcttttt ctttataaac tttggtttaa cttattcatt
      121 aggtttactg ttatattata ataaatttat tgatccagaa aactttgcat cattcatgat
      181 gatattacct ctatcttcag ttgccattgc aaagttttat actgaaggat taaataatga
      241 tatgtatgaa ttttattcac taatcatatt attttttctt atttattcat tattaattgt
      301 tcttaagtta ttaaatctaa taaataataa tgaatttata attataaacc taatattaac
      361 ttttataagt agcttatcaa ttataatata ttcatcaaat aaaaaaaatt tatatcctgt
      421 aaaaaatatt aaactaggtt ctttattaat tatttacttt atgatttctg taattgttcc
      481 aacatcattt atactaatac tacaaggggc tcaacctaat tatagtggta tcataaaatt
      541 tatatttact ccaataacat cattaactac tatttatatt ttttttagtg aagaatatgc
      601 ctggagaggt tttttacaga atattttttt tgataaattt ggtaaaaagt taggagttat
      661 aatattaggt atgtgttgga gcttgtggca cttaccacta atatttacat tatatacacc
      721 tgaagcgcca atattaggaa taatattacg ttcaatacat atagttggaa tttcaatatt
      781 tttaggatat ctttatataa aaactaagaa tatctggttt tgtgctataa ttcatgtttt
      841 aaataatagt gcttttctta tcacaaattc taaagaatca actatcactt accatgatat
      901 actaatagta tctatggttg ttttaatttt ttatgttcca tttctattta caaaagaata
      961 taaaaataac ttggagtaaa tagaatatcc aagttatttt tatatttttg caaatttcca
     1021 acaatcctac agaatacttt aagcttagaa gatattatta aaatttatta taatcaacta
     1081 attcttgata ttggtcacat ttactttatc actaacaaaa tcatacttag aatctctata
     1141 gtaataccat acaaatataa agctaagtag attaacaaaa gtagtcaaaa ttccaccttc
     1201 aataccaaac attcctccat ttaatagatt accatgagtg atttgtgtag ttataatccc
     1261 tggaacttga agtccactta caggcatacc atatataatc ccctgaaata cattccatgc
     1321 aaaatgataa cctatacaca tccatatatt cgacgatttt ataaacatat atgaaaaaag
     1381 aatcccaata attataatat taattacaga caaaaaagta acatcaggat tccatatatg
     1441 aatcaaccca aaaataacag atggaacaat cattataaaa taaatatttt tacatcttcg
     1501 taaaactgcc ataaaaaaac cacgatttaa aatttcttct gcaaaagcaa ctaaaataaa
     1561 gaccagtatc caagaaactg ttaaaggtga aatttttggt acccatgaag taacatgacc
     1621 accaccaatt gttattgtta taataaagat tactgtacaa ttaaatattc ctaataacat
     1681 tcctacatat ccttcttttt tcttaattct ataagaatgt aatcccattt cactaatagg
     1741 atttttcata ataaatctcc atacaattat aggaataata atcataagtg aatctgttag
     1801 aatttgaaat acaatctgta acccaacatc atttaaccaa ttagcatatt ctgaaaaata
     1861 gtttgttttt aaattaatgt cacctgtagc aaccaggatt tttcttatta tttccacaag
     1921 tatcatgctt aaataatact gaagtatgta aaaggtagcc ataacagcta taataatcca
     1981 ccctgaacgt acttttttgt ctttattctt aataatcaag ttgctccatg atttgttcat
     2041 atagatgtct tcctcctaac tattattttt agttgtttct aaaatatatt ctattaaatt
     2101 ttttcatagt agtattttac cacaaaactt ctgcttcaat gaagtaagcg taatatattc
     2161 tctaactgta atttaaacta taaaaaatat ataaagtata tttaaagcaa tatttatgga
     2221 cataaagcaa tataaacaat atataaaaca tataaaactt tacttaatta tttctacata
     2281 aaaaccacat aaaaaaggat gtaactctag cttttagtgt aacatccttt ttttaattaa
     2341 aacttgtatt taattttaga aagctgtctc atgaccaaca taacaagcta taccattatc
     2401 caaaaatata tcttgtaatt cttcaagatg accttcatca acatcaccct tatcagaata
     2461 ctcatattcc ttgcctaatt gaatccattt tgattctcct aatctgtgaa atggtaatag
     2521 attaatttct accaaattat ttttgtgcat aaaagatata atatcactta tattttcaac
     2581 actatcatta aacccagaga taactggtac tctaagtact aatcttccat tccaatcaga
     2641 atttgcaagg tttgatatat tttctaatat taaatcatta tacacacctg tttgttcttt
     2701 gtgtttttct ctatccatat gctttatatc aataaatgca aaatcaatat cgttaaatat
     2761 tttattaaaa acttcatttg atacacatgc actcgtttca atggcagtgt gtatatttac
     2821 ctcatgacac tttaataata cttcatgtaa aaattcatgc tgaagtagtg gctcacctcc
     2881 actaaatgtt actcctccat tacttctcca attgttagaa tcacgtttaa ttacctgcac
     2941 aagttcatct acagtgtaag gttttgcaca taatttaaac gcattataat aacatgagtt
     3001 gacgcattca aaactttcac agtccttaca gatgttccaa tctataactg gcttattatc
     3061 aagattaaag ctcaatgcac catttttaca cttaccatga catacagtac atccattttc
     3121 atattgacaa gataattcac taaacatcat atgtggtctt acagtccaac tttctggatt
     3181 tgcacaccat ttacaactca gtggacatcc atttaaaaat acagttgttc tacaacctgg
     3241 accatcatga actgaaaagc tttgtacatc aaaaatcatg ccttctaatt gcttttgact
     3301 actcattaaa cacatgtcct tcacaagtaa ttgcattcaa tgttgaatat acccagtctt
     3361 ctttctctag gccaacacat ttgcctatac cttcatcatt agcttcaaca aaatttttac
     3421 aatttttaca ttttggcatt acttttatct ctggacatgc tgcatcatct aaattaatca
     3481 tttgttttga taatctacaa attcctttgg ttgcatctac tgcacaaaaa ttcatacaat
     3541 cactatgctt tctcatattt tacacccctt catactctgt tctagcaatt acttcatctt
     3601 gtattggttt acctaattca caccaatatt gagtaaaccc agcaacacgt accattaatt
     3661 gacgataatt gtcaggattt ttttgtgcat cttttaatgt ttctgaatct acaacattat
     3721 attgaatatg gaacccacct tttcttaaat aacttcttgt taaatctaat aattttttag
     3781 ttccttgttg tccttttata gtagttggat gaagttttaa attcatctga gaattttgaa
     3841 ctactgcttg gtcccaaata gttgcagatt caaataatgc atatactcca tttttatctg
     3901 ttcctgcata tgcagagact gaaccatctg aatatgttgt tccacttagt ctaccatctg
     3961 gagtagccaa ggtagcagct ccctgtggcc catgtgtaga aacagatatt tggcaagggt
     4021 acatcttctt agcatataaa gattgtgctt cttcacatac ttttgataac catacctcat
     4081 agtttttaag aatattatct gcatacaaat cattatttcc atatttaggt gcatcaagac
     4141 aggccttata aatagcatca tatttaccag tcttatctac ttttacttgg tctgccatag
     4201 aataatttcc tacttctaat gcatctttaa atccaaaatt atttaatatg gcatccttca
     4261 tttcctctat tgtaaatgct ttgtcatcat atacaagttt cttaagtgct gcaaatgaat
     4321 tgaccatagt aacagtacca caagtttcta cgtttaatgt tgcattataa cgatatccca
     4381 tatttcctat atgttgacct ttgtctaaac agtctggctt tagtagtgaa tttattatag
     4441 aagtatcaaa ttttctccat atatctaact caatgttatt tgctctttct aaaacattta
     4501 ttgcttgttt ataataatct ttaaatactt ctatgacttc ttcataagta tctaatggct
     4561 tattatgagg ctcaaatact tgtatattca tacgatagtc tttaccattc ataagaacaa
     4621 gttctaaaat ttttggatta gctataaagt gaactccaga gccagcagat tgacctgctc
     4681 cacctgcaat agagtatgtt tttccgttta aagttaactg tttccaacac cctggagacg
     4741 tttccaaaca tccacctaat gcaaatgctc ttgcttcttc aacagtcata ccttcatcag
     4801 caaattgttt catcataaat gttgttccat ttgagttgtt tacccatgct ggataaccag
     4861 aaccaagctt tgtacattca gcagctttca ttaagaaatc ttctggaagc ttttcatcat
     4921 atagcatagt taaactaggt tgaggagttc tacatctcat acttgcttct aataataact
     4981 cttctagttc attagctcct gtagaaccat cttcttttaa tccacctata gataaagtat
     5041 taaatgtatt gcctgatagt actcctccat ttacgcccgc tgaagcaaaa cagtcaatac
     5101 aagtgatttt tattctataa agctctaata attcgataac ctcttctttt gtaattagtc
     5161 ctttttctat atcttgttca taccaaggat ataaaatttg accaaaacgt cctatagaca
     5221 taccagatat agcatcctca tttactgatg ctatatgaat tgtataagtc aactgaagag
     5281 cttccctaaa tgtacgaggt tgtttatgtg caatatgttc taatatctcc actaaatctg
     5341 agtattcttt tttagcttct aaatctgtct ctatactttc taaatatttt gcatgttttg
     5401 cataatttaa aatccaagtt tgtaatcctt caataacttg aataacagcc tcataaaaat
     5461 atagacggtc cataccaata agcccatctc cagatgcatt tcctgcaact gcctttttac
     5521 attcttttgc catttctata attccatcta atccataatt taaaggataa aaataattta
     5581 taacttctct accttgagga agtgtatacc ctgaatcaaa catgcatata agtgtactca
     5641 ttaggttttc ttttaaatca taatctggca tcattttttc atagtgaaaa cctaaatctt
     5701 caacagactt tccaacccat tcctttgcca tttttacaag tacaggtact tcttcttttc
     5761 tcattccgaa tttaccagct attgaaacga catttccaaa actttcagta acatttcctc
     5821 caccagaccc aaattttgta agttcatcag ctgtgttact ggctaatttc tttgcctctt
     5881 ctctcatttg ttctccttga gcaacaaaga agctttcact tacccatggc attggaaaag
     5941 aacctctata atggcgtgtc ttttgcatta ctattttctc accaggtatt atatttggtg
     6001 tcatatgtga aaaagcagct ttaagagctt tagctcttct aactactggt atatcaccat
     6061 ctgattttct atattctcta ttataccaat atggaaattc catatcagca gtagataatg
     6121 tattataata tacatctaat aagtcttctg ctcttttact tgcttttttt tcattaaatt
     6181 cacttaaatt ttctttattt tgaaaatttg atttaatatt ttttgcttcc aaaatacttc
     6241 ttattttgtc ttctttactt tgactcattt cttcccctcc ttaatctttc tat
//
LOCUS       DAYAOY010000159         6246 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYAOY010000159 DAYAOY010000000
VERSION     DAYAOY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.163"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(392..799)
                     /locus_tag="ACGI6B_003769"
     CDS             complement(392..799)
                     /locus_tag="ACGI6B_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HFG9753176.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1273..2157)
                     /locus_tag="ACGI6B_003770"
     CDS             complement(1273..2157)
                     /locus_tag="ACGI6B_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9753177.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2448..2924)
                     /locus_tag="ACGI6B_003771"
     CDS             complement(2448..2924)
                     /locus_tag="ACGI6B_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFG9753178.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3190..4365
                     /locus_tag="ACGI6B_003772"
     CDS             3190..4365
                     /locus_tag="ACGI6B_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFG9753179.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4446..5249)
                     /gene="proC"
                     /locus_tag="ACGI6B_003773"
     CDS             complement(4446..5249)
                     /gene="proC"
                     /locus_tag="ACGI6B_003773"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFG9753180.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5289..5879)
                     /locus_tag="ACGI6B_003774"
     CDS             complement(5289..5879)
                     /locus_tag="ACGI6B_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9753181.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2058 a         1079 c          706 g         2403 t
ORIGIN      
        1 tgaacttctt acactttcat gtaagcacag actatatctt atccttcagc attatccgtt
       61 aaggtctacc cacttccact accaatagct tgtagtgtac ttccctcaag aggaatagtc
      121 gttgaagttt ctcctattcg gagtttacct gctgattgcc cattttaaag tacttaggat
      181 ttaaccttat accatctaac caattttttc tactttcgta actttcacac ttaggtttat
      241 ttcatcctta tgttttagtt tgattagctt tagggatttc cagcaattca agtagtattg
      301 gataggttct atcctatctc tacatatagg tttccctata tgctgactat tttagctact
      361 aactcatgac taaagtcacg agtgtgcgta gctatttttt aatcaatatt ttagtaaatt
      421 ttacggtaag taaaaacctc tctccttcaa atcctatagc aaatatactt acaagttgct
      481 ttgagaaaat aactcctaat atagctacaa ctatgcatat accaactatt atatttaaaa
      541 cattgtttaa aaactttagt gcttgctttt caccttgttt actacttata tcttgataca
      601 ttggaataaa agtagtaact atagcagtac ctattgctgc aaatataata tttggtatat
      661 tcatagctgt taaataactc tctgtatata agcctgtacc ataaattgat gataatacta
      721 attctctacc cattccaaga atcttagaga ttatagtaac tatcatcaaa taaaaagttg
      781 ctttagctac cttgctcatt ttatcactcc acttctaaca tacctctttt taatcaagta
      841 aaatttagct cattagatat aattctatca actaatttgc cttgcttagc cctatcaaaa
      901 tataattaac acctatttct taatatatca caaactacat tattcttcat atttttttat
      961 ttctccaata agttttacat ttttcctata taaattgtat agcttcaatt atattcaaag
     1021 aaatggagtt gctacccgat aactttaaaa gtaaaaaaca gattttatta tctttttaaa
     1081 ctatattcta acatattttg tccatattga gaaattttac taaactatta tgtactttat
     1141 taaataaata aaataacagc acacctataa aatacaagtg tgctgttcat aaacagattt
     1201 tataaaaaaa taattcctaa gtagagatta acatgctttc tcatactctc atatataatg
     1261 tgtctaaaaa tattaataaa aatgctttaa ttgttttaac ttacttggat gtcttaattt
     1321 tcttattgct ttagcttcta tctgtcttat tcgttctctg gtaactccaa atacctttcc
     1381 aatctgttct agtgttttag ggtcatcatc atcaattcca aatctcattc ttaaaacttg
     1441 ctgttcttgc tctccaagac ctgtaagtag ttcttctatc ttttctttta aattatgctg
     1501 ttcaacttcg tgtattacaa catctttaaa atcagcatct gatggtataa attcaactaa
     1561 actactatcc tcatcttctt ttaatggagt atccaaagat acaacatttt tatctcttct
     1621 taataattcc aacactttgt caacttctaa gccacatgca tctgcaatct catctatagt
     1681 tggctctctt aataatacat ttaaaagttc ttgtttcttt ttctttacaa aatttattct
     1741 ctgatgaaga tgtataggta tcctaatagt attttcgcaa tcgtcgatat atcttgttat
     1801 actttgtttt atccaccaag ttgcataagt gctaaatttg tatccttttt tataatcgta
     1861 cttttctaca gcttttataa gtcctatatt tcctgcttga attaaatcca acatatcaat
     1921 actatctctt ttatatctct ttgctatgct cactaccaac ctataatttg aatttataaa
     1981 tttttctttt gctactgaag atgagttgat tatttcctta gccagctcta cctcttctcc
     2041 tgctgacaac attttgtatt cctctatttc ctttaaatac attttcatcg cattcgatac
     2101 atttgatgaa cccacacaaa tataattttc ataattattt tcttttttag tatccataat
     2161 caaacccctt ctattcatag aatttgctaa aacaaaaata aataatttaa acctacataa
     2221 tctacaatta cataatatcc cattctttaa atgaattgtt ttgtatatta tgtatacaaa
     2281 tttataaaaa ctatacctat aatattattc tacttctaac agcaatatcc tccttatata
     2341 tataatttat tttattatat gttgttatat tcttagtttt ttgaatgata tatgtctaaa
     2401 aaaagactgt ttactaaaca gtcttttttt atatatatgc tcaatattta attttcatga
     2461 tgatgattac atccacatcc acaattttca tcatggtgat gatgttcttc taaatctttt
     2521 acctgttgtt ccatttcatg aaactctttt accattatat cataagttgg aagagctgcc
     2581 tcagtatcgt attcttcccc tctccaatca gcgtacatca tagcatctcc attagttaaa
     2641 accaatctac ctgacatagt tatattatcc acttcttcta aaaattctat ttgtttttgt
     2701 aaaacttctc cttctaactc cacatcttca tctaaacatt gcactatcat tacaggtgca
     2761 taataatctt cttcatcttt aacatttaca acaatatcta aaattccttc tgcaccttct
     2821 tcaaattctg ccaactcaac atttgtatca atcatatctt gaggtactaa taaatcttct
     2881 attaaatatt ttattactct atcttttact aattcttttg acatttattt ttctccttcc
     2941 gattctataa cattatcata atcatttcta tattatagta tactacatta attgtttttt
     3001 caatctctta caaaaattat tttttattat cttccttata ttcccttttt tattacaaag
     3061 ttgtaatcgc attgatttaa aaaatagttt atatttagta taatagaaat gtagcacata
     3121 tttttattca atatattaat aaaaaaatat attgaaaatg taatgtatgt taaatctagg
     3181 aggaatatta tgaagtttaa aaaacaaatt agttctattg ttgtgtctac tatgcttgta
     3241 cttgggagta tgaacctttc ttatgcagac ggtacaaatg tagttacact tggagctaac
     3301 ttatcagagt ctcaaaagca gcaaatgctt aattactttg gagtaaaaaa agaccaagtc
     3361 ttagttttag atgtaacaaa tgcagaagaa agacagtatt tacaaggtgt tgcaacagag
     3421 ggtcaacttg gaaaagttac tatctcttgc tcatacgttg aacctactaa aaaaggaaat
     3481 ggaattaatg ttaaaacagc aaacttaact tgggtaacta gttcaatgat tgcttcaacc
     3541 cttacaacag ctggtcttga agatgcaaat gttattgctg ctgctccata tcctgtaagt
     3601 ggtactggag cattgacagg aatcatgaaa gcatttgagg atgcttcagg aaagaaactt
     3661 gatgaaacta agaaagaaat tgcttctgaa gaattggtag ttacaggtga cttaggagat
     3721 gatatagggc aagaaaaagc aacaggtgta atgaatgata taaaaacaga aattgtaaag
     3781 aatggtacaa aagataccaa tcaaatagca aatacaataa ataatgttgt aaataattac
     3841 aatattacta ttacaccaga acaaaaagaa caaattaaag gtgtaatgaa aaaaatagct
     3901 gaccaaaatt acgattacaa tagcatgaaa aatacactag ataatgtatc agataatgta
     3961 aatgaacaat taaagaaatt aggtgaaagt gttaagggtt ctggtatact agatactatt
     4021 ggtggatggt ttagtggaat aggagactgg ttctctggtt tattctcagg cggagataaa
     4081 aaagatttgg gaatcttaaa taatactaat gatgagtctc ttggctctga tgctgtaata
     4141 aattctacag aaggtgttca aataaatcca caaaataata ctaattctaa caatgatagt
     4201 gatacaaata gctcaaaaaa tgatacttca agtaataatg aaaataacga tagttctcaa
     4261 acaaatgaaa acaacacaac aaataattca gaaaatcaat caaataataa tgactcagtt
     4321 ggtttctttg aaaaaataaa aaattggctt tcaggcttat tttagaaatt aaacaaataa
     4381 aaagaattgc ctaaaatgga aattttctat aaaaggcaat tctctttatt tacaataaat
     4441 ttttattact cactcatttt tttagatttt tctatacatg cacatattgc ttttatcaca
     4501 gaagctctaa aaccttcttc ttcaagtact ttaactgctt ctatagtagt tccaccaggt
     4561 gaacatacca tatcttttaa ttcaccagga tgttttcctg tctcaagtac catttttgca
     4621 gaacccataa cagcctgtga tgcaaactta tatgcttgtg gtcttggcat tcctgcaatt
     4681 actgctgcat ctgctatagc ttctataaat aaaaatacat atgctggtgc agaaccactt
     4741 gcaccaatta cagcttctat aagatactct ggtactacct cagtatttcc aaaagaatta
     4801 aatacttctg taactgcttc taagtcttct ttgtttatat ttttatttat cgaaagagaa
     4861 gacatggctt catttacaag agctggagta tttggcatag ttctaactat ttttttatct
     4921 tctcctataa tagactctat atcttttata gattttcctg ctgctattgt aactataatt
     4981 ttattgtcat ctataaagtc ttttatctct tccaaaatat catcatatat atctggttta
     5041 acagctacaa ttactatatc agaggttcta gttacttcct tcgagtcttt tgtagtattt
     5101 attccaaaat ttgcatctat tctatcgaga gttccttgac tatatgctga tgctataacc
     5161 atattaggtt caactaattt tgaatttact attcccccta tcatagcgct tgccatattt
     5221 cctgcaccta tgaatccaat ttttttcact ttagtacctc cttattgact aatagtaatc
     5281 gttaaaattt aagcttcttt aactgcatca gatttttcaa atggatttgg tatacttact
     5341 accaaaattt tcactacctt attagtatag ttgacccaat tatgattgtt cttttcacta
     5401 ttatagtgta ttgtgtctcc tggatacatt tctatttgtt catctcccaa aaacactgta
     5461 agagtaccct ctaatacata cacaaattcc tcaccttcat gtacgtaaca acaaatttcc
     5521 tcactactct tacttggaag aatttcaatt aatcttggta gcattgtttt ttcctttaaa
     5581 ctagatgata aatgataatg tataaatgtt gagttttcta catcaaatac ttctttttca
     5641 taacttctta atacagctct tttatgctct tttggcttca taaaaaagta agttaattct
     5701 acatctagta cacttgctat ttttcctaag gaatctgttg ccacacttgt taatcctctt
     5761 tcaagttgtg ataaaaaacc aattgacaag tttgtttttt cactcatatc cttcagcgtc
     5821 atttgctttt gagttctgag ttgctttatt tttgctccta tgtccttatt caaaaccatc
     5881 acccctaaat aattttaatt ttttcataat ataaatacaa aaatataaat ataggtactg
     5941 aacccatctt tatttcattt aaaaaaattt ttataagtat tactataatt tatcaatctt
     6001 tttttgtcta catttttacg attatttacg atttattttt cacatatgta tatttaatct
     6061 tgccaactaa agatacattt tttataagca tttactcttt atataataaa acttaagttg
     6121 ttcatgtagt cttcttctgg tttaattatt aaaatttcaa attatattat tatttctaca
     6181 cattattaca tcctttagta caattataac ataattttac aatttaatga tatctattca
     6241 atgtct
//
LOCUS       DAYAOY010000160         6207 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYAOY010000160 DAYAOY010000000
VERSION     DAYAOY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.164"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            134..577
                     /locus_tag="ACGI6B_003775"
     CDS             134..577
                     /locus_tag="ACGI6B_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9753182.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            608..2425
                     /gene="ftsH"
                     /locus_tag="ACGI6B_003776"
     CDS             608..2425
                     /gene="ftsH"
                     /locus_tag="ACGI6B_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFG9753183.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2603..5305
                     /locus_tag="ACGI6B_003777"
     CDS             2603..5305
                     /locus_tag="ACGI6B_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9753184.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5339..6037
                     /locus_tag="ACGI6B_003778"
     CDS             5339..6037
                     /locus_tag="ACGI6B_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9753185.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         2442 a          698 c         1143 g         1924 t
ORIGIN      
        1 caatattgta attgacaata aatacttaat actataatat ttaatacatt aaacattata
       61 gtgttaagta tttatttaca gtatgcactt cagcctaaat ttgaaataaa gtattttcgt
      121 agggagcgtt tgtatggatg agatgagttt taagaaagag ctactagagt tgactagaga
      181 tataaatatg aaatttacaa ctttattaag tgatttttat cagccacttg gtattacagc
      241 agtgcaagct ttgattttat ctgagttatg tgaacatgga gagaaaaaaa taagcgattt
      301 agggaagaac ttaaacatga caaatagtaa tgtatcagta atctgtcaaa ggcttgaaaa
      361 aaatggcttt ttaaatcgca taagagatat agaagaccaa cgaattgtaa aagtaaaagt
      421 tacaaataaa tcattggata tacaagaaca tatttcaagt agtatttttg atagttattt
      481 tgaaaatatg accagtgaaa aattgcaaga tatggaagat ataatagaag gtttagagaa
      541 attaaataaa cttttaactt acattgattg caaataacat aaaaataatt taaaggagaa
      601 taaaattatg gatgaacata aaccccaaaa aaggtcagca ctctattatt atacagttat
      661 aataatagta gtaatgtttt taaatgcttt aatatttcca agtttaatgg ataaaaaaat
      721 ggttgagatt gactatggaa agtttctcac aatgttggat acaggaaagg ttaaatctgt
      781 tgagatagaa aataatagaa tagcaataaa tccatctagt agcaaggata agaatatata
      841 cataactggt agaatggatg acccagaatt ggtagataga ctaaaaaatg caaaagtaga
      901 atttactaag gttattccta aggaaaattc acctctttta agtattttac ttacatggat
      961 aatacctata ggtattctta tgctatttgg taacttaatg atgaagtcta tgcaaaagcg
     1021 aatgggtggt aatgctatgc aatttggaaa aagcaacgcc aaaatgtatg tatctgctca
     1081 aagtggaaaa agatttaatg atgtagctgg tcaagatgag gcaaaagaag ctttaactga
     1141 aatagtagat ttcttacaca atccagaaaa gtataaaaag ataggagcac aaatgcctaa
     1201 aggagcactt ttagtaggac ctccaggtac aggaaaaaca ctccttgcaa aagctgtagc
     1261 aggtgaagca aatgtaccat ttttctcaat atctggttct gagtttgttg agatgtttgt
     1321 aggaatggga gcttctagga ttcgtgattt atttaaacaa gcaaaagaaa aagctccatg
     1381 tatagtgttt atagatgaga ttgatactat tggtaaaaag cgtgataatg gaagtggatt
     1441 aggtggaaat gatgaacgtg aacaaacttt aaatcagttg ttaagtgaga tggatggttt
     1501 tgatggtgga attggagtag taatacttgc tgctacaaat agacctgaca gtcttgacaa
     1561 agcattactt cgtccaggaa gatttgatag acgtatacca gttgaacttc cagaccttgg
     1621 aggacgtgaa gctatattaa aggtacatgc tcaaaatgta aatgtaagct cagatataga
     1681 ttataatgca attgcaaggt ctacatcagg agcatcaggt gcagaacttg caaatattgt
     1741 aaacgaagca gcacttttag cagttaagtc tggcagagat atagtagaac aacaggattt
     1801 tgatgaatct gttgaaactg taattgctgg gtatcaacgt aaaggagctg taatttcaga
     1861 taaggaaaag aaaattatag catatcatga aataggtcat gcacttgttg ctgctatgca
     1921 aaaacattct gcacctgtcc ataagattac tataatacct agaacttcag gagcattggg
     1981 atatactatg caagttgctg aagatgagag tgtacttatg tctaaagaag aagcaattga
     2041 taaaataact acttttacag gtggtcgtgc tgctgaagag gttatcttta acacttgtac
     2101 atcaggagca tcaaatgata tagaacaagc tactaaaatt gcaagagcta tggttactcg
     2161 ccttggtatg agtgaagagt ttgatatggt cgctcttgaa actgtaagca atcaatattt
     2221 aggaggagat acttctcttg cttgttcttc cgacactgct gctaaaatag atgaagaggt
     2281 atttaaaata gtaaaggctt gtcacaaaaa tgcaattgaa atcttagaaa ataatattga
     2341 taaattgcat gaacttgcta attatttatt agaaaaagaa actattacag gtgatgagtt
     2401 tatggaaata cttaacttaa actaaaatga ttatacacaa gataaaaatg cctattgtta
     2461 ggcattttta ttttacaact aaatttttta aatttatatt taaatcttta cataatctta
     2521 atcattaaaa atacatctta actacaattt tatatcggaa ttgatattat aatagtaatg
     2581 aatggtgtaa ggaggttttt taatggatag acctaatcct gatattctat tagaaaaaat
     2641 taaaaatgaa gaagaaaaac taagtagagg tcaattaaaa atattctttg gttatgcagc
     2701 tggagtagga aaaacctact ctatgttgga atctgcacaa aatttaaaaa aagtgggtgt
     2761 agatgtagta gttggatata ttgaaccaca cactagacca gaaacattgg ctttacttga
     2821 tggcttggaa attcttcctg taaaggagat agaatataaa tctataaaac taaaagaatt
     2881 tgatttagat gctgctttag caagaaaacc agaagttata ttagtagatg aatttgccca
     2941 ttctaatgtt gctgggttaa ggcatacaaa aagatggcaa gatatagaag aattgctgtt
     3001 agcaggcata aatgtctaca ctactgtaaa tgtacaacat ttagaaagtc taaatgacat
     3061 tgttgagata attacaaacg tatcagttaa agagactata ccagataagt ttttggatac
     3121 aaatacacag ttagaactta tagatgttga acctgatgta cttcttgaaa ggttcaatga
     3181 aggtaagata tataagaaag aacaagctac tagagcaaaa aataattttt ttataaaaga
     3241 taacctggtt gcacttagag aaattgcact tagaaaaaca gcagagagag ttaataaaga
     3301 agttcaaatg accagactat caaagggtga tgtaactgta atacctacat cagatacact
     3361 tcttgcatgt ataagtccat ctccatcttc agcaaaagtt attagaactg catccagaat
     3421 atcagacagt acatttgcaa aatggatagc tttatatgtt gaaactccaa acacagcaag
     3481 acttccattt gatgaacaaa aacaactaca aaataaccta aaattagcta agaaattagg
     3541 tggagaaata attgttttac atggtgaaaa tataatagag caaatactta gaattgcaaa
     3601 gctgagaaat gtaacaaaaa ttgtaatagg tagaaatcat tcaagtaata aaagatttag
     3661 taaaagattt aaaaaagata tagttgacaa attaattgat gaagttgatt atatagatat
     3721 acatataatt ccatataaat ttgcctcaga tgttaaatac aaacctaaaa aggataaatc
     3781 atcaataaaa tctaaattta aaattagcaa agttgatttt ataaaactta tatttattac
     3841 tttgatgata agtattttag catatgcagt acaaagcatg ggatttataa gagaaaatat
     3901 attgttaata tatatgttgg gtgtagtttt agtttctatg tggactaaag ggtatagtac
     3961 aggtataata agttctgtat ttaatatagt tcttttaaat tattttttta cagcaccatt
     4021 atatacatta tctatagcag attcaaacta tatagtaaca ctggttgtat tctcaatagt
     4081 aggtataata actagcactc ttacgtctaa gatacagcat gaagcagaaa ctgctgctaa
     4141 aagagaagaa aatacaaaaa tgatatatca aataattaag ggatttttaa gattatcaaa
     4201 taaagatgat atagtaaaca aaggtataga acttttgtct cttagcttaa gtagagatgt
     4261 aatttgttat ttaaatgtag ataagcataa taaatcaaag ctttacaaaa aaaatactaa
     4321 taataaggat aaaaatgatt tggatagtga agatgaaaaa gctgttgcaa gttgggtttt
     4381 atccaattca acagtagcag gaaatgacac ggatacactt cctggttcaa agggatacta
     4441 tataccaatt gtaggaatga atttaacttt aggagtagtg ggaatatcat gtattgattc
     4501 aaaattggat acagaagata taagcttaat tgaaacaata attgctcaaa tggcaatagc
     4561 gcttgataga gaaatcttgt cagaagctaa agaaaatacc aatcttgaga ttgaaaggga
     4621 aagattaaga agtaatcttt taagagcagt ttcgcatgac cttagaactc cactagcagg
     4681 aatatctggt gcagtaagta caattataaa aaacaagggt actataggtc aagatataat
     4741 agatgaatta cttagtggtg tatatgaaga tactcagtgg ttaataagac ttgttgaaaa
     4801 tttacttagt atgaccaaga tagatgaagg gaaattggaa gttaaaaaac attctgaact
     4861 ggttgaggaa attatatcag aagcgttaca aaaaataaaa aaaagaattg aaaatgcact
     4921 tatagatatt aatataccag aacagatatt atttgttcct atggatgcaa aattaataga
     4981 acaagtttta attaatttaa tagataattc tttgaaatat tcaaaggaag attgtaagat
     5041 agagataaat gtatatgaga aagatgatta tgtttggttt gaagtatctg ataatggtcc
     5101 tggtatatca aaggaactta aaaagcatat ctttgacaga ttttttactg gagaagaagg
     5161 agctaaagat tccagaaagg gtgttggact tggattatca atttgtaaat ctataattca
     5221 agctcataaa ggtgaaatta tggtagaaaa caataaagat aagggttcaa cttttaagtt
     5281 tagtttgcct aaggaaaatg aatagtttta aattttaaaa ttagttttgg aggaagaaat
     5341 gagtaaaaaa cttatattgt tagtagagga tgataagaca ataagaaaat ttatatctac
     5401 tgcactttta acacaggatt atgatgtcaa ggaggctata actggaaaag aaggtatatc
     5461 tatagctgtc tcatattctc cagatgttgt tcttttagat ttaggcttag aagatatgga
     5521 tggaatagag gtaataaaag ctatcagaca attttcaaat ataccaataa ttgtagtttc
     5581 agctagagag caagatagag acaaggtaga agtatttgat gcaggggcag atgattactt
     5641 aacaaaacca ttctccatag tagagttatt agcaagagta agagtagcat ttagacattc
     5701 acaagtagaa gctcaacaaa aagatgatgt taaatctacg tttgaagtag ataaattatt
     5761 gattgatttt gataaaagaa aagttattgt tgatgatgtt gaagtccatt tgacacctat
     5821 tgaatataat attctttcat tacttgcaaa gtatcatgga aaagtgctta ctcataattt
     5881 tataataaaa gaaatatggg gtagtgttat agggaatgag actaagtctt taagggtctt
     5941 tatggcaacg ttaagacgaa aaatagaaaa acaacctgca aacccaagat atataattac
     6001 agaagttggt gtaggataca gattaaatga tgaatagttg taatatatta attattagat
     6061 tatgtgctta ttaatatata ttaaaattat atagtttagt taaaataaga atgaagtgta
     6121 tgtgcttgtt gtcagtttag cacatgcatt ttatttttta gcttttttag tatcaataat
     6181 tttttagtat caataatatg aaatatg
//
LOCUS       DAYAOY010000161         6167 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAYAOY010000161 DAYAOY010000000
VERSION     DAYAOY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.165"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(205..2799)
                     /gene="clpB"
                     /locus_tag="ACGI6B_003779"
     CDS             complement(205..2799)
                     /gene="clpB"
                     /locus_tag="ACGI6B_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFG9753186.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEAIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2836..3432)
                     /locus_tag="ACGI6B_003780"
     CDS             complement(2836..3432)
                     /locus_tag="ACGI6B_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753187.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3449..3853)
                     /locus_tag="ACGI6B_003781"
     CDS             complement(3449..3853)
                     /locus_tag="ACGI6B_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFG9753188.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4220..4594
                     /locus_tag="ACGI6B_003782"
     CDS             4220..4594
                     /locus_tag="ACGI6B_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9753189.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4591..5454
                     /locus_tag="ACGI6B_003783"
     CDS             4591..5454
                     /locus_tag="ACGI6B_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753190.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            5454..6092
                     /locus_tag="ACGI6B_003784"
     CDS             5454..6092
                     /locus_tag="ACGI6B_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9753191.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2045 a          931 c          707 g         2484 t
ORIGIN      
        1 gaacaatagt ttttataatt atattcaaat tatgattata ttataagaaa tttactaaaa
       61 tacttccttt gagttaaatt taatttggac atattgactt aaaaatgttt tattaatttg
      121 ataaaatatt tcttttccta tagtttataa ataaaaatca gctgagcaaa atatttagta
      181 tattatgctc aactgattct tttattattt aactaaaact tcaatatttt catcttttcc
      241 atctataact atagtacatc cattatacac atctccagca ataagtttct ttgcaattat
      301 agtttctaat gtatttccta tatatctctt aagaggtctt gctccatata cagggtcata
      361 tccttctctc accataacat cttttgctga atttgtaact tcaatagtaa tatctttttc
      421 ttttaatcta ttttttaaat ctttcataaa tatatctata attttcttga tattttcttg
      481 gtctaaaggc ttaaacatta taatatcatc tactctgttt aaaaactcag gtttaaatct
      541 atgcttcatt tcattcatta ctaaattgtt agtagtctct gttatatttc ctcctgcttc
      601 taatagataa ttacttccta tattagaagt cattattata agagtatttt taaagtctac
      661 tgtcttacct ttattatcag taagtctacc atcatctaat atctgtaaga acatgttaaa
      721 tacatcctca tgtgcttttt ctatctcatc aaatagtatt acagaataag gagcacgtct
      781 aactgcttct gtaagttgac caccctcttc atatcccacg taccctggag gaggtccaac
      841 tagtcttgat acagcatgtt tttccatgta ttcagacata tctattctta ttatattatc
      901 ttcactatcg aataaatttc tagcaagagt ttttgcaagt tcagttttac caacacctgt
      961 agggcctaag aaaataaatg aaccaattgg ttttctttca tcttttaatc ctgctcttgc
     1021 acgtattact gcatttgaaa ctgctgtaac tgcttcatct tgtccaataa ctcttttatg
     1081 aagttcatct tctagtttaa gtaatttttc tctttctcct tcaactagtt tagttactgg
     1141 tatacctgtc cactttgata caatgctaga tatttcttct tcagtaactt cttcttttaa
     1201 aagagaattt tcagaaccat cattcatatt ttcttcgtac tgctttattt gttcttctaa
     1261 cttaggtatt tcaccatatt taacctctgc tgctttgttg aaatcatatt ctctttcata
     1321 cttttcagct ttacctttag cttcgtctaa ttgagccttt aagtttttaa tatctaatat
     1381 ttgactcttt tctttttcat attttgctgt catttcatca tttttagatt ttaactctgc
     1441 tatttctttt tgaatatcat ccaatcttga tttactttta tcatcatttt ctttaagtaa
     1501 tgcttctctt tcagtttcta atgtaaataa ctttcttcta acaacatcta attctgttgg
     1561 caatgaatct atttcactac gaatcatagc tccagcttca tcaattaaat caattgcttt
     1621 atctggtaaa aatctatctt gtatatatct atgagaaagt tttgctgctg ctacaatagc
     1681 attatcatgt attcttactc catgatgtat ctcaaatctt tcttttaaac ctctaagtat
     1741 agatattgta tcttcaacag taggttcttc tgcaattaca ggctgaaaac gtctttcaag
     1801 agccttatct ttttctatat actttctata ttcatcaaaa gtagttgcac caatacagtt
     1861 caattcacct cttgcaagca taggtttaat aagattacct gcatccattg aaccttctgt
     1921 tttaccagct ccaacaatag tatgaatctc atctataaac agtataattt ttccatcaga
     1981 actttgaact tcttttaaaa ctgcctttaa tctttcttca aattcacctc tatattttgc
     2041 accagcaatt aaagctccca tatcaagtga gaatacaatc ttatctttta aaccttctgg
     2101 gacatctcct cttactattc tctctgctaa cccttctaca attgcagttt tacctacacc
     2161 tggctcacca atcaatacag gattattttt tgttcttctt gataaaattc ttataatcct
     2221 tcttattgct tcatctctcc caattacagg gtctaattta tttttcttgg ctaaatctac
     2281 taaatttgtt ccatatctag caagtgcatc atatgtgcct tctgggtctt gtgtttctac
     2341 tctttgacta cctctcactt gtgataaaat atttaaaaag ttattcttat ttatattata
     2401 ttgttttaat attttaccaa cagctgattt tgattctgtt tccatcattg caagcataac
     2461 atgctctaca cttatatatg aatctttaaa ttctttagaa atactctctg cctttataag
     2521 cacctcattt atttttctag tagctgatac tccttgtgag tcagcacctt ctccatatac
     2581 ctttggaagc ttatcaattt caaagtttac agtatttctc aaagaatcaa ttgatatatt
     2641 cattttttcg attatatttg gtatcaatcc atcctcttga ttaataagtg ctgagaaaag
     2701 atgaattaca tctacttgtt ggttatgatt ttttactgct tcgttataag cctcatttaa
     2761 acttttttga actcttaaag tcattttttc tacgtccata taaatccctc cattttaata
     2821 ttatatccaa ataaattaaa ttctctcatt tataattatt gattttctaa gctctaaaag
     2881 actatcaagt aaattctcgt ttttttgtat atcgaaactc aatgttaaat taaatgttcc
     2941 atctaaatta aaaaaatcga atacttcttt tgattttgct attaaataca agtcttcatc
     3001 tataagtctt ttttcaccta ttaataaata ctgacattta tctataaaac tctttttaaa
     3061 atcaatagtg tatagatttt ctaaaatatt atattgataa atagcaatat tattaaacac
     3121 ttttaatatg tttgaattta caaaattgta aatttgatta ttgttataca gtgatatttg
     3181 ttcgtatata tattccctaa ataaaggatg aatgttttgc ttaagtttta aagtaacttc
     3241 agctggtatt tcaaaataaa gcttccagta cttagagtta tttttttcta aaataaaagc
     3301 tactttgttc aaactggtag aactaatctc ctttagtgta caattaaaat tatagtgtct
     3361 caaactagtt ttttcattct caaatacata gtcaaaccac tgtagaacat ttctatcaag
     3421 ttttaaactc atataaaaac cttcttcctt atttactaac cttattaggt ctttttatct
     3481 gattatattc tgctaataaa ctttctttat cagctctact acctttatat gctatatgtg
     3541 gatttatcat atactcacca tttttcactc tttgtaaaaa atccattttt ttcagctccg
     3601 taaacaatga agataaagaa gtttttggat attcaaattc acaagataat tcatataatg
     3661 tttttctaat cttattattt ttatccatag agtttattaa ttttaatgga attaaatccc
     3721 tcttagtata cattccttct actactttaa tataattctt aaaacaaaca ttcatatact
     3781 tacttttatt cataattaaa cttttgctct ctgtatattc ctgatttaaa tcctttttta
     3841 atgtcttatc caaattaaat atctcttctc actctcctta tctctaattg ataatataaa
     3901 tatattacaa aaaaatatgt atgtcaaaac tgaaaatgca aaaagttcac cttataagta
     3961 aacttttatt tagctataag ctaaacttta ttattttttt aatttagtat ttccaattta
     4021 ttggcaggat ttttgaacaa tatttataaa ttttttaaaa aaatttaaat ctatttaata
     4081 tggcagactt tcataaaata cattacattt attaaatgta taatattggt aattagtttt
     4141 ttctaaaatc agttgacata ttatatatat atttatataa tgtgtatata ctatatatac
     4201 aatttaagga ggtgtcttat tgaatatagt tattagtaat tcgagtggga agccaatata
     4261 tgaacagatt actacacaaa ttaaaagtat gattataagt ggagagttac cagaaggctc
     4321 agctcttcca agtatgagac tattggcaaa ggaacttaga ataagtgtaa ttactacaaa
     4381 acgagcatat agcgatttag aacgagacgg atttattgag acagttactg gtaaaggcag
     4441 ttttgtatct agtaaaaaca tggattttgt caaagaagaa caatttcgtt taattgaaga
     4501 atacctacaa aaagcagttg atacagcaaa aagtagtgat attacatatg acgaattaaa
     4561 agatatttta tttttattat ataaaggaga atgataagta tggatgcaat aactataaaa
     4621 aatttaaata aaacttataa agattttagc ctacaagata tatctttttc agtaccaaaa
     4681 gggagtgtaa tgggatttgt aggtgaaaat ggtgcaggaa aaacaactac attaaaagca
     4741 attttaaatt taatatctta tgatagtgga aatatagaaa tctttggtct tgatagtaaa
     4801 aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat ttgagggaag caactttcat
     4861 gaaaatttaa acactgacca tgtttcaaaa atcatgagta aaatctataa aaattggaat
     4921 gacacattat ttaaagacta cttaaaaaaa ttgagagtac cagacaataa attaattaag
     4981 gagttttcaa aggggaataa gatgaaactt agtattgctg tagccttatc tcataaacca
     5041 aaattattaa tactagatga ggcaactagt tcacttgacc ctatcgttcg tgaagaaata
     5101 ctagatatat ttttagattt tatacaagat gaagaacact ctattatctt atcttcacat
     5161 attacaagtg atttagataa gatagcagac tatattacat ttatacataa aggaaaaatt
     5221 gtatttagtg aaaataagga tgaattaatt gatactatgg gagttttaaa atgtaaacct
     5281 agtgattttg ataacctttc aagagaggat tattcatatt atcgtaaaag ccaatttgga
     5341 tatgaagtac ttcttaaaga taagcataga tttatctcaa gacatccaaa ctgtattgtt
     5401 gataatacgt ctatagaaga aattatgtta ttttatgtga gaggtgataa ataatgaaag
     5461 gtctaatttt aaaagattta cttaatttga aaggaaatat aaagtttata cttttattca
     5521 tcattatgtt tggctttatg tcttctttag gagatggaaa tgttaataac tttataggtg
     5581 ttattatagt tttatgtact acaatgattg tttcaacatt ttcatatgat gatttgaata
     5641 aatgggatag ttatgtactt acaatgccta ttaatcgcaa tgatattgta ttaagtaaat
     5701 atttaactat gttaatattt agctttatag gtgttcttgt ttcacttatt gttagtgtaa
     5761 ctattggtta ttttaaaaat acacttatat taaatgaaac actacttatt aatgcactaa
     5821 tcttatcaat ttcagtatgt tttggtagcc ttattctacc acttatttat aaatttggta
     5881 cagaaagagc tcgtttatta atgattttat gctttttagt tccaacacta gctttattag
     5941 tttttaaaag tattttagaa aatattagtt cacctatttc tatagaaatt atacttaata
     6001 cactagtata ttctcttcca tttgttgcta ttttactatt tgtgatttca tattttatat
     6061 catcaaaaat ctatagtaaa aaagaagtat aaaatatact atatatgttt ctgtatttaa
     6121 gtatttaaat aaaatactat cgagtttatt ttggtagtat tttattt
//
LOCUS       DAYAOY010000162         6117 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYAOY010000162 DAYAOY010000000
VERSION     DAYAOY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.166"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            786..1187
                     /locus_tag="ACGI6B_003785"
     CDS             786..1187
                     /locus_tag="ACGI6B_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HFG9753192.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1250..2251
                     /gene="rsgA"
                     /locus_tag="ACGI6B_003786"
     CDS             1250..2251
                     /gene="rsgA"
                     /locus_tag="ACGI6B_003786"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFG9753193.1"
                     /translation="MEYHDLYLARIISQEKNFYQAVCVNGVLFAEVSGKLRFEATIQS
                     DFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAGKVNEEQVVAANIDIVFLCM
                     SLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVHRKLQVVNSIALGVDVVVTS
                     SIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMGGEFLNTKEIRIDDDKGRHA
                     TTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFADIEAYFGKCHFRNCTHTSEP
                     GCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLAKKEKKFKNISKINKKIRR"
     gene            2787..3485
                     /locus_tag="ACGI6B_003787"
     CDS             2787..3485
                     /locus_tag="ACGI6B_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753194.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            3663..4553
                     /locus_tag="ACGI6B_003788"
     CDS             3663..4553
                     /locus_tag="ACGI6B_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753195.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(4946..5902)
                     /locus_tag="ACGI6B_003789"
     CDS             complement(4946..5902)
                     /locus_tag="ACGI6B_003789"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9753196.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         2301 a          703 c          996 g         2117 t
ORIGIN      
        1 tacaataata caatagaatt gcttattatt caatatactt atttgaaaat taaataaatt
       61 tacaaatatg aacgttattt tatatcaaaa aaaatagact atgcatgtag aatatatgga
      121 aaaacttgat tgtaggtttt gtatgaaaag acagttgtta ttaatttaag tgagataaat
      181 tgagcaacag gcataaaatg agaaaacaaa taatagcagt taatttttat tattaatact
      241 aggtgaacat taaatttcaa aataccacta gattttatag aataagaaaa tgagacaaac
      301 ataaatatat gaatatatta aattatactt gaacatactt atattttatg ataaaatttc
      361 aataataatt attagtgaag gagtgtcttg cctgatgtca gagttatata taatggtaac
      421 caacaaataa atagttggag acgcatatgg gttctgttta atcagaattt ttaagtgtat
      481 ttttgataca tttgattgtt gtgtcttact ctgcctttat agataactaa cattagacaa
      541 caaataataa tattacatat tactttgtta aattgtggtg tagtgggctt taatctgcta
      601 cacttttatt aattttacac agtattattt tgaatataat tatgttgaaa tagaagatac
      661 taaattttct agtccacagg ggttttctgt ttttgtgtag gtaaatatgt ataccacata
      721 aaaaggcaga tataaatacc tgtggacttt ttttgtagaa aatacacgat acggaggaaa
      781 taaaaatgaa ttataaaaat agaaaacgag cttattatca ggaaaattcc tgtatggata
      841 actttatttg caaaaattgt ggttgccttg tagttcctga aggagctggt acacaacata
      901 gaaatcattg tccacattgt ttacatagtc ttcatgtaga tatcattcct ggtgaccgtg
      961 ctgcagattg tgatgctaat atggaaccta ttggagtgtg ggtgagaaaa aatggtgagt
     1021 gggcaattat tcatcgttgt acacgctgtg gacatcttag ttctaatcgt attgcagctg
     1081 atgataattc agtgaaatta atgtcaattg cattaaaacc actggcacag ccccctttcc
     1141 cactagaaag aatcattgaa aagttggaaa aggaggaaac agaatgaatg gtatgaaaca
     1201 aatcaattta caagactatg gtctaaatag aaattttatt actcaaagta tggaatatca
     1261 tgatttatac cttgctcgga ttatctcgca ggaaaagaac ttttatcaag ctgtatgtgt
     1321 aaatggtgta ctgtttgcag aggtttcagg aaagctgcgt tttgaggcta ctatacaatc
     1381 tgattttccc acagttggtg atttcgttat gttggatcgt tgcaataata aaaatggtaa
     1441 tgctattatt catcatgtgc ttcatagaaa aagcttgttt gtgcgtaagg ctgctggaaa
     1501 ggtaaatgag gagcaggtgg ttgcagctaa tattgacatt gtttttttgt gcatgtcact
     1561 gaataaggat tttaacattc atagattgga gaggtatctg tctttaacat gggaaagtgg
     1621 agctatccca gtagtagtac ttactaaatc agacttatgt gacaatgtgc atcgtaaatt
     1681 acaagtagtt aattctatag ccttgggtgt agatgtagtt gttacctcat ctatagagcc
     1741 agatggttat aaacagattt ctaagtatat taaaccaggc tgtactgttg ccttacttgg
     1801 ttcttcagga gttggaaaat cttcattaat taattgtctg atgggtggag aatttttaaa
     1861 tacaaaggaa attcgcatag atgatgataa aggaagacat gccacgacta gacgagagct
     1921 atttttaatt tcaggtggtg gtattgttat tgacacacca ggtatgagag agttgggaat
     1981 gtgggatgtt tcagaaggtt tagataaaag ttttgcagat atagaagctt atttcggaaa
     2041 atgtcatttt cgtaattgta ctcacacctc agaaccaggc tgtgctatct gggaagctat
     2101 tgctcatcaa gaactttcgc ctaaaagatg ggaatcctat cgtaggctta aaactgaagc
     2161 agtatttgca gacaataaaa gcgaatactt agcaaaaaaa gaaaagaaat ttaaaaatat
     2221 ctcaaaaata aataagaaga ttagaagata agtgtagttt ttttgttcat tctatgagtc
     2281 tatatactat taatatatta aatttgccat caatttgtta ctttaatgtg attttgatgg
     2341 caaatttggt ttgtggtatt atttaaagca atttattaat ttttttaata aagtttgatt
     2401 ttttatatga tgatatatac atgtgataat gtatacatat ttgttacgat aggctccttt
     2461 ataaagaggt cttatgtaag ataagaaaag gcatatttat actaatacat aatagagtat
     2521 atgcttatgt atgatagaga tgtttcttaa gagcaaaact gcaatattgc taatttataa
     2581 agtaaaaata tatgaattat aaaatacaaa taatatattt ataaaaatta ttgttttaat
     2641 ttataataaa aattttttgt tgaaaataat gctcgaacct ataaaactat taactaatat
     2701 ttgtaaatgt atacttttga tgataaaata taattaagtt aagttggttt ttataaacat
     2761 aaatttattt taaggaggtg aaataaatga aaatatctag tcaatataga agtcaatatt
     2821 catttagata tgaaagtaat ataaataata caagaataaa tgaaagtatg gttaagaaaa
     2881 atgaaactgt aggaaaagac acttatttat ctaatattat gaaacaaaag caagaactta
     2941 atgatagaat tagagattta aaatatagac aagaggttta tactaaaaaa ataaatgacg
     3001 caattaagaa cttatgtaaa tcagaaataa gagaaacaac taataatttt tctaatatag
     3061 aaataggtat taaaaatagc attatagaag agaaaaataa aagtacaatg ttagatgaaa
     3121 attcaactta tctaaataca aatgatgaaa aagaatcttt aatcactaaa gagtctaatg
     3181 aaaaaattga agaagaaata ttaaatgatg aaaaattaga agagttagaa caaaaaaagg
     3241 attataaaga ggattctaat aaaaaagaga aagtatctga ggacttatct ttagtaggta
     3301 aaactcgtga agagcttgaa aatatgctta aaaattttat aaatttaaca caagaagaaa
     3361 taatgaaact tgagtcgaga atagaaaagt tagataaaaa tgctgaagaa tacaaacaaa
     3421 attcaaagac taatatattt gataaaacag atgaacaaaa aaaacatata aatgtactga
     3481 tttaaacaat atgttttaaa aaaataagtt tattatataa tgaaaatata aaggtacaat
     3541 ttcaatacac ttctattaaa ccaataattt atgaaccaga cttaatttca atttactaat
     3601 aaccatatat ttatgagtta atagttttat gttttaaaaa gaattaaata agggggagta
     3661 gtatgaagaa gaatatatta actactatga ttaccatgat tattatattg atgatgagta
     3721 tgacagtaat ggcagagcca tatcaagaag atgaaacatc tagcttgaaa aaacagagtg
     3781 caaatgaacg taatcagact aatataggca caatggatgg cagagtgcaa acaaatgcaa
     3841 taagtaaagg tgctaattat gaccctaaat atcctcttaa aggaatgtta acacaacttg
     3901 gtcttaatac aacaggtaaa gagggatggg atttactcta tggaggaaat cccaatggag
     3961 ttgagcttcg agcagatggt aaatattatt atataaataa gggtatggaa gggctaggat
     4021 tttatgcggc agcattgtca ggtggctcat atcagagaat aggctttgga aaacttgacc
     4081 ctatagaagt aatagctgat ggaaattgga tttcttacaa acaagctcaa gatacactta
     4141 cagtaatcag gaatttttta aatagctttg actggagaaa tgcatctgag atggaaagag
     4201 ctaatagagc tgcaaaattg gtaaccgaag caaaatatgc agactctaaa tattgtaaca
     4261 tagtctatgg taatctggtt gataaacgtg gtgtatgtgg aagttttgca agtagctttc
     4321 atttattaac aaggttaatg ggaatggatt cactatctat attaaatcca tcattaaatc
     4381 atgcttggaa ctatatacaa attgatggga aatggtatag gtttgatggg tctgaaatat
     4441 cagcttttgg tggatcatta gactttgatt accgtaaatt aaaggatgca acaagagaga
     4501 tgactactta ctatgatgct aaggcattgt ctatattagg atttaatcaa taaaaatagg
     4561 gggaggcttc ttatagagcc tttttttatt gatttgaaat ttaataagga attttatatg
     4621 aaaatttaat caataaaagc aaaaaaggct ctttataaag cctttctaag tgttttttta
     4681 agtaaaattt tttatatcaa catcagtttt aatcaaaaca tataacattt tggacttact
     4741 tttaaaaata aaattattaa aatataatat taatcatgtt tttagtttat tatattctat
     4801 aatagtggtc atgttgacta ttgtagtttt attaagaata gttggtttat ataaatatat
     4861 gcaaagatag cttgttgttt actttactta ttagtatata ggacaagata ctctaataaa
     4921 tttgcctatt ttaatatctt ttgtactaaa accaactaaa gtttccttta ttatttcgtt
     4981 ttgaataata caaagcataa tccgattgtt tgtataaaga ctggaaagta tcagagttat
     5041 tttctaattt agatatacct atagaacaag aattactctc taggcataat tctaaaacct
     5101 tctttgcctt attttcaaca ttctcttttg attgattact atattcaaaa acagcaaatt
     5161 catctccacc aagtcttcca ataatagcat catttacgaa tactttagtc aaaatatttg
     5221 aaaaatgtat tagtatttca tctcctcgaa catgaccaaa gatatcattt acctgtttaa
     5281 aattatctaa atcaaagatg taaaagtatc catgtgaaac agttttaagc atttctttta
     5341 tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat aaggtctttt tctgatttta
     5401 gtttaaattc atttgccatt gctagtgctt cttgtgcttt ttcagttatt gttttagggt
     5461 aatactcatc tttcatttgg tcaaagtttg gaatagaatg gtgaacatgg ataagtttaa
     5521 tcatatcatc tgtaactctg tataccatag taaatcgagt atccatttct acatagatat
     5581 ctttttctag gtaatctttt tctattaccc aaagagtacc atatactata aaagcttcgt
     5641 ctgtgacttt ggtacatgaa taccactcat ctataatctc aaagtctatt tgctctgatt
     5701 ctgtttggtc tttttcaaga cctataataa attcttctaa tgtttcgtaa aattcatgtt
     5761 ttcctgttcc aattaatgca aggttgtcat ctaataagga aataataaat ttaaggtttt
     5821 cttcttctgg atttaaaata taaatatgcc aaattttttt tgataaatct cttaattgtt
     5881 tttgtattgt attattttcc atagattacc cccttaagat ttacagttta tattttaatg
     5941 tattataagt tgaactctta ttacaatatt attataatat agaactaaag aaattacatc
     6001 ctgtataggt aaaagtatat cttttacaat aaaaatatta cagtaaaatg accagtttta
     6061 atatacgtat tatattttta atttataaac gaatattata aatgaaattt aaattca
//
LOCUS       DAYAOY010000163         6089 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYAOY010000163 DAYAOY010000000
VERSION     DAYAOY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.167"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(312..2144)
                     /locus_tag="ACGI6B_003790"
     CDS             complement(312..2144)
                     /locus_tag="ACGI6B_003790"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753197.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2137..4383)
                     /locus_tag="ACGI6B_003791"
     CDS             complement(2137..4383)
                     /locus_tag="ACGI6B_003791"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753198.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4451..4912)
                     /locus_tag="ACGI6B_003792"
     CDS             complement(4451..4912)
                     /locus_tag="ACGI6B_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9753199.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5217..5783)
                     /locus_tag="ACGI6B_003793"
     CDS             complement(5217..5783)
                     /locus_tag="ACGI6B_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFG9753200.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1958 a         1106 c          643 g         2382 t
ORIGIN      
        1 atataatcat caactattaa aattaaattc tatttataat aaaatttaaa gaataaaatt
       61 aaatatacat tttgataatc acctccaaca ttttccttac tacatattat aataaaataa
      121 acactttgta aagacataaa attatgtggc tatctcaaaa tatagagaat cctctttttc
      181 attgttaaaa tgataaaaat acactttatt tcatacacaa aagagtgcct tatgaattaa
      241 aaagttcatt ttaaggcacc ctcatttatt ttatcttatt aattacatgt ctatgctaag
      301 atagtttttt gttacattgc ttcatcttct tcaaattgag aattgtaaag attggcatag
      361 aatccgcctt tctttagaag ttcctcgtga tttccttgtt caactatatc tccttcattc
      421 attacaagaa tcatatcagc atcacgtata gttgataatc tgtgggctat aacaaaactt
      481 gttctacctt ccataagatt atccatagct ttttgaatca atacttctgt tctagtatcg
      541 acagaacttg tagcttcatc taatattaaa attttaggat cagctaaaat agcacgagct
      601 atagtcaata attgtttttg accttgagaa acattacttg cttcttcatt taatttcatc
      661 ttatatccat ctggtaatgt cttaataaat ctatgaacat gtgcagattt agcagcctct
      721 ataacttctt catctgtagc attcaactta ccatatctta tattttccat aattgaacca
      781 ctaaataacc atgtatcttg aagaaccata ccaaacattt ctctaagctc actacgatta
      841 aaatctttaa tattatgacc atcaatcagt attgaaccgc tattaacatc ataaaatctc
      901 attaacagtt taactattgt agttttacct gctcctgtag gtccaacaat agcaactttt
      961 tgaccaggtt taacatttac tgagaaatca tttattatag tcttgtttgg gttatatcca
     1021 aagttgacat tttcaaaatc aattttacct tctaaaccat ctattgaaac tgcattttca
     1081 acaatttgga cttcttcttc ctcttctaaa aactcaaata ctctttcaga agctgctgct
     1141 gttgattgca ataaattggc aacctgtgca atctgagtca ttggctgtgt aaagtttctt
     1201 acatattgta taaatgactg tatgtcacca acctcaattg ttttcttaat tgcaagccaa
     1261 cctcctaaga ttgataccat tacatatccc aagtttccaa taaacatcat aagtggttgc
     1321 attatacctg ataaaaattg agatttccac gctgaattat acaggctatc atttagttta
     1381 tcaaactctt caattacttc atcctcacga ttaaatgctt ttactattgt ttggccactg
     1441 taagtttcct caacttgacc atttacattt cctaaatatt cttgttgata tctaaagtat
     1501 ttttgtgatt tctttattat tcttgaaatt acaaacattg atattggtaa tattaaaact
     1561 gccactaatg tcataattcc acttatagat aacatcatta tcaaaacacc tatcatagtt
     1621 gttactgatg ttataagctg tgtcatactt tgatttaaac tttgatttaa agtatctata
     1681 tcattcgtaa ttctcgataa tatttcacca tgcgtcttag tatcaaaata cttcattggc
     1741 aatctatcca attttgctga aatctctttt cttaaattat aggatacttt ttgagaaatt
     1801 ccactcatta taaatccttg tataaaagag aacagtgcac ttatcaaata aaggaaaagt
     1861 aaaaaggtta agatttttcc aattgcatta aaatctatac ctacgtttcc tccagaaact
     1921 ttactcacta gcccttcaaa tatctttgta gttgcctttc ctaaaatttt tggacctact
     1981 atagaaaacg acgcactacc aatagcaaat acaattacta gaattattga tattttaaat
     2041 ttagataaat atgcaattag ttttcgcata gtccctttaa agtcatttgc tttttgtcct
     2101 gctcccatag attttcccat aggacctctt cccattctac tcattagcta gctcctcctt
     2161 tgaaagttgt gataaagcta tttgctcata aacttcgcaa ttttttagaa gctctttatg
     2221 tgttcctatt ccaactattt cgccctcatt taaaacaatt atctgttctg catttaagat
     2281 agtactaatt ctttgtgcaa ctattagaac tgtactttca gcagtttttg attttaaagc
     2341 cttacgaaga gctgcatcag tttttaagtc aagtgctgaa aaactatcat caaatatata
     2401 aacttctgga ttttttgcta ttgctcttgc aatagaaagt ctttgttttt gacctccaga
     2461 tacatttgtt ccaccttgag aaatttcagt tttaaaagct tccggtttag aatctataaa
     2521 ttctgtagct tgagctattt cagctgctgt tctcatttct tcatctgttg cgtcttttct
     2581 accatatttt aagttggatt ctatagtacc agaaaacaat actccctttt gaggtacata
     2641 tccaattttt tcccttaaat catgttgaga aacatttttt atattaactc caccaagtaa
     2701 tatttctcct tcagtaacat caaagaaacg aggaattaaa tttataagag ttgacttacc
     2761 actaccagta cttcctataa aggcagttgt ttctcctggc tttgctgtaa aagatatatt
     2821 tgttaatata ggttcttctg cgtctggata catgaacgaa acatttttaa attcaacaag
     2881 acccttttta tcttccttaa actcttgtgc ctctgatttt tctgggtctt ttatgcttat
     2941 atctgacact aatacttcat ctatacgtgt agctgaaacg gatgctcttg gaagcataat
     3001 agataccatt gatatcatta agaatgacat tattatttgc attgtatatt gtataaatgc
     3061 catcatatct ccaacttgca ttgctccaga atcaacactg tatgcacctc tccaaactat
     3121 aagaactgtg ataaggttca ttataagcat catagcaggc atcatacatg tcataactct
     3181 atttacaaaa agatttgttt tagttaaatt tttatttgct ttatcaaatc tttcttcttc
     3241 atgtttttca gtactaaaag ctctgataac tggaatacca gtaattattt cacggcttac
     3301 taagtttaac ttatcaacaa gcttttgaac cagtttaaac ttaggcataa ccactgagaa
     3361 taacactaca attaatgata agatagcaac tactgctact gctataatcc aggccattga
     3421 agtatttgtg tttaaaacct ttatcactcc acctatagcc attataggtg catagaatac
     3481 tattctaagc aacataacca tcaacatttg tatctgttga atatcatttg tactacgagt
     3541 aattaatgaa gctgtagaaa attcattcat ttcagtattt gaaaatgaca taacttttct
     3601 aaatactcca cttcttaaat ttctacctac acttgcggct actcttgctg ctaaaaatcc
     3661 tactgttatt gtagctacca tactaatcat tgctatacca agcattttag caccagtaaa
     3721 taatatataa ttattttgag ttttttctgt atcaatacct attttctcat attctgacct
     3781 tacatatgaa acagagcttt gtgttatcat actctctggc atactttcaa agcctttgct
     3841 catctcttta gtcaatgtat ctaactgttc ctttggcatt gctgataaca atttaaatat
     3901 atcaacatct ccactttgag tagcttgtgg tggaagtttg ctcattaact gagctttcat
     3961 agcttttacc tctttactat cgccctcaag ccctgatact attaaaatag ctttaccaaa
     4021 aataacattt aaatcatcta tagtgtcttt gtctttagta tttaactcat aaagagattc
     4081 atcttttaga cctggatact tatctttata cttaacataa tcctttttat tcagcaaagt
     4141 ataattatct agtactttat ccttactact tttatccata aataaagtaa ttttatttaa
     4201 ttcactctct ctaatcacag atggaacaga gttttctact ccaccttgtt gtatacctac
     4261 atttactata ttggatgtgt attctggtaa agaaaggtca cataccgctt gaattaccaa
     4321 tagacagact attattacta ttgataatgc cgatttcttt aaatatttaa ttaatttaaa
     4381 catacatttc ttcccttctg taatattaat tagtctaata acattaaatt gaaaaacatt
     4441 attttaaact ttaaaaatat ttttcatctt catgcttatg acatcctgct tttaaattat
     4501 ctcgtacttg cattagaagt cttctaagta ctattttttc ttcttcacta aatcctaaaa
     4561 aaaactctct ttccaattcc ttgttaaatt cttcaacttt attacatatt tctaaacctt
     4621 tgtctgtcaa aaaaatccta gatactcttt ggtccttttc atcttgtttt ctttccacaa
     4681 gtccagcttt ttccatcctc tttatcataa ctgtagttgt agatgctttt actttccttt
     4741 tatctgctaa ttctttttga ctttgcccat ctttcatctt taaagaaaaa agtatttggg
     4801 gttgaccatg atataagcct aatttatcaa aataaaaatg agttcttata aaatatattc
     4861 gcattaattg caagaaaaca tcatttaatt tttcttcaga attgctattc atacttgccc
     4921 tccttatttt ttatattact tagtcggcta agcattattt tactatactg catgcatttt
     4981 ttcaatatat ttataaaaaa ttcatataaa tttcatttat taaatatttt tatatcatat
     5041 agttataatt ttaataaata atagtaagat ttttaattag tcagctactt atatttttta
     5101 ttgtaaagct taaaataaac cttttcatat attttcaaat aaaaaagttg tcccaaaata
     5161 aattttgaaa caacatcata ttcaacagta tcttgattat ataacattat gatactctac
     5221 ttatcttcat atccatttac aacaacgtct aacttcccag tttcaggtga tataattaga
     5281 ccatgtacaa taataccatc tggaattaaa ggatgccctt ttattaaatc aatactatct
     5341 tttacagatt cttctgcaga atcgaatcca tgtaaccatt gttttgtatc aattccagca
     5401 ctacgaagtg taagtattac atcattagat attcctctat ctaaaatttt accaagtaac
     5461 ttatccgtat caacattaca cataccacat ccatgatgcc ctacaaccat aacctcatct
     5521 acatcaaatt cgtaaattgc aactaaaaca cttctcataa tacttccaaa aggatgcatt
     5581 atagtagctc ctgcattttt tattagtttc acatctccat ttttcaaatt catagcctta
     5641 ggcaaaagct cagttaatct tgtatccata caagataaga ctactatttt tttatttgga
     5701 tgttttgatg tcacatattg ttcatattct ttgttctcta caaatgattt attatattct
     5761 aatatctctt caagttttct catatgtaat atcaccctct ttaaaaattt ttttattatt
     5821 atatcactat taaagtacaa tatatacaca tataatttaa tttatattta ttaaattatt
     5881 ttataagatt attactctac tataaatctt gtatataact tgtatttata agtatttgta
     5941 tatttatttt ttataatcta aactaatatt atatattttc attatttttt taattataat
     6001 ttttttaaaa ttttattcta aatgccttac ttataattaa ttttttattt cacctatata
     6061 taattagatt taaaaatgtt taccttacc
//
LOCUS       DAYAOY010000164         6083 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYAOY010000164 DAYAOY010000000
VERSION     DAYAOY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.168"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            227..853
                     /locus_tag="ACGI6B_003794"
     CDS             227..853
                     /locus_tag="ACGI6B_003794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753201.1"
                     /translation="MKKITKLILCSLLVISIASPLQVEAKTTHHSSSHVSIHKSSTKS
                     KSSRPKITKTKKATKTYRTNKSIKKKIKLKKQVNKKTQVPNKQAARKDKKTTTYNYYN
                     SYNSNRYYTTSSSGLSFWDCYMLGHLFNNNNKVSEQDIARELQKQGYSKQETDNILRK
                     ANQNQAKKEEKKKRVMMLIFSVLGALFIIGLGLIIFIKRESKGCNKSM"
     gene            926..1615
                     /gene="terS"
                     /locus_tag="ACGI6B_003795"
     CDS             926..1615
                     /gene="terS"
                     /locus_tag="ACGI6B_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFG9753202.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKHNVSLSTVKSWATRYWKQKG
                     CNQSKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELMQEISIKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELK
                     ISVDYGDRNDS"
     gene            1593..2846
                     /locus_tag="ACGI6B_003796"
     CDS             1593..2846
                     /locus_tag="ACGI6B_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9753203.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSSTHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNIFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            2933..4291
                     /locus_tag="ACGI6B_003797"
     CDS             2933..4291
                     /locus_tag="ACGI6B_003797"
                     /inference="COORDINATES: protein motif:HMM:TIGR01538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9753204.1"
                     /translation="MEHYILKHRPEKYKQGEEYYYGNADINNKRRYYLLDGAKVDDFT
                     KVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDDNLTKLLNDLLGEEFDDTITELYLN
                     ASNKGVEWLHPYINRKGEFKYVIIPAEEAIPIWDSKRQRELVAFIRFYYIEDIDGNKI
                     KRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMTDIQEGHFRINNKEQGWGKVPFIPF
                     KNNEKCVSDLTFYKSLIDIYDNNISTLADNLDEIQEVIYVLKEYPGTSLQEFIDNIRY
                     YKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKNIIIFGQGVNPESQNTGDKSGVALK
                     FLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKISGNKSYDYKTVQITFNHSMIINEA
                     EKIDMAAKSTGIVSDETIVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVID
                     ET"
     gene            4281..5642
                     /locus_tag="ACGI6B_003798"
     CDS             4281..5642
                     /locus_tag="ACGI6B_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9753205.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIEKDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKVLV
                     NEFSKKFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCHCRTTTAPWFEDEEGYRAARDENGKTYYVPSGMKYK
                     EWYEKTFITDKKDDILSNKSWLKADFPTEKKFKKHVEKHLNEYGNITAEEYLSTARNL
                     LSEQLSNDVEGFLSKDNFLFKYRKSTNDFAVGRADGKISTLFKPKEGYNYWLEQIKEY
                     KEV"
     gene            5645..5806
                     /locus_tag="ACGI6B_003799"
     CDS             5645..5806
                     /locus_tag="ACGI6B_003799"
                     /inference="COORDINATES: protein motif:HMM:NF025569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFG9753206.1"
                     /translation="MNCPVCGKDVDVFDICDNCNWQNSGPKETNYKGPNKMSLKEAKE
                     AYEKGKKVI"
BASE COUNT         2544 a          655 c         1119 g         1765 t
ORIGIN      
        1 tatcttgaat gtgaagacaa acctaagtta attgaatttt ataaggataa tggatttgta
       61 gattttggta aaagaagtct tgataaagat gaaacagatt cattggatgg ggactatcta
      121 gtccaaatgt taaaatatct aaaaaaataa aagtacataa aatctaaaat gactatcttg
      181 atggatggtc tttttttata caataaatta aaaggagaat tatagaatga aaaagataac
      241 taaacttata ttatgttctt tgctagtaat cagtatagcg agtccattac aagttgaagc
      301 taaaactact catcattcca gttcgcatgt ttctattcat aagagtagta caaaatctaa
      361 aagtagtaga cctaaaatta ctaaaactaa aaaagctact aaaacatata gaactaacaa
      421 atctatcaaa aagaaaataa aacttaaaaa acaagtaaac aaaaaaactc aagttccaaa
      481 taaacaagct gctagaaaag acaaaaaaac aactacatat aattattaca atagctataa
      541 ctcaaataga tactatacta cttcaagtag cgggttaagt ttctgggatt gctatatgtt
      601 aggtcattta ttcaataaca acaataaagt ttcagagcaa gatatagcta gagagttgca
      661 aaaacaaggt tatagtaaac aggaaactga taatatatta agaaaagcaa atcaaaatca
      721 agctaaaaaa gaagaaaaga aaaaaagagt gatgatgtta atattctctg tattaggagc
      781 attgttcatt ataggtttag ggctaataat atttatcaaa agggaatcaa aaggttgcaa
      841 taaatcaatg taatttaatt agatacatag actctaacaa gagttctttt ttattcccaa
      901 aacgacaaac aaacgaggtg gtggtatgaa tgaaaaggca gatttagccc atgaagatta
      961 cttaaaaggg cttaagtaca aggaaatagc tgaaaagcat aatgtaagtt tatcgactgt
     1021 aaaatcatgg gcaactagat actggaaaca aaaaggttgc aaccaatcaa aaaaagttgc
     1081 aaccaaaaag agaggtgcac ccataggtaa taaaaatgct actggtccac caggtaataa
     1141 aaatgctgaa aagtttggtt tcttctcaaa gtatctacct gaagaaacta gggaattgat
     1201 gcaagaaata tctataaaag ataaattcga tattctttgg gaacagataa caattcaata
     1261 tgcagcaata ataagagcac aaaagataat gtatgttaaa gacaaggaag aaatgattaa
     1321 agaattaaag aaacatgaaa gtacagaaaa tggagagaag atagagtatg aatttcaatt
     1381 tgcatgggat aggcaagcat cttttcttaa tgcacagagt agagctatga gtgaattaag
     1441 aagtttaatt aaacagtatg atgaaatgat tcataaggat tggaatttgg ctacagagga
     1501 gcagaaaaca agagttgaga agttgaaatg tgaagttgat aacctaagta aagatgacat
     1561 tggagatgat gagttgaaaa taagtgtaga ttatggtgat agaaatgata gttagagtaa
     1621 attttaatcc agatttcaag gaagccaatt ttactaaaaa aagatacaga gcaatgaaag
     1681 gttcagcagg gagtggaaaa tctgttaatg tagcacaaga ctatatacta aagttaggag
     1741 ataagaagta tcaaggagct aatctattag tagttagaaa gtcagaagct acacataagt
     1801 attcaacgta tgcagagctt acaggagcta taaatcgtat ttatggtaaa caagctgata
     1861 agtattggaa aactacttta aatcctttag aaattaagag taaagttact ggtaactcta
     1921 taattttcag aggagttaat gatgcaaaac aaagagaaaa attaaaatca attaacttct
     1981 cgaaaggaaa attaacatgg gtttggtgtg aagaagctac agaacttatg gaaagtgaca
     2041 tagatatact agacgaccgt ttaagaggta ttttaactaa tcctaaccta tactatcaaa
     2101 tgacatttac atttaatcca gtctcatcta ctcattggat aaaaagaaag tattttgact
     2161 ataaaaatga tgatatattt actcatcata gtacttatct acaaaataga ttcatagatg
     2221 aggcttacta cagaagaatg caaatgagaa aagagcaaga ccccgaaggg tacaaagtct
     2281 atggtcttgg agaatgggga gaaactggtg gagcaatact taaaaactat gttatacatg
     2341 aatttcctac ggaatttgaa tactttgata atatgagact gtcacaagac tttggcttta
     2401 atcatgcaaa tgtagtactc agaattggtt ttaaggatgg agagttatat atatgtaatg
     2461 aaatatatgt acatgaaatg gatacatctg aaataataaa gattgcaaac agtaaaggtt
     2521 tagaaaagaa tatatttatg tactgtgata gtgctgaacc agatagaatt aagatgtgga
     2581 agagtgcagg atataaagct aaaggagtta aaaaaggacc aggaagtgtt aaagctcaaa
     2641 tagattattt gaaacaatta agaatacatg tacatcctag ttgcactaat accataaaag
     2701 aaatacaaca atggaaatgg aaacaagatg aaagaactgg attatatctt gatgaaccag
     2761 ttgagtttat ggatgatgca atggctgctc ttagatattc tatagataat aagcttaaaa
     2821 ataatggaat aagcttctta aagtaaagga ggcgttaaat atttatataa gtgaaacaga
     2881 tttaataaaa gttcagttaa aaaaagagag cacatttaac ctagtaaaag ttatagagca
     2941 ttatatttta aaacatagac cagagaaata taaacaaggt gaagaatact attatggcaa
     3001 tgcagatata aacaataaga gaagatatta tctcttagat ggagctaagg ttgatgattt
     3061 tactaaagtt aataataaag caattaacaa ctaccataag cttttagttg accaaaaggt
     3121 aggctatagt gtcggaaatc ccatagtatt taatgcagat gatgataatc tcactaagct
     3181 tttaaatgac ttactaggag aagagtttga cgatacaata acagaactat atctcaatgc
     3241 tagtaataaa ggggttgaat ggttacatcc atatattaat agaaaaggtg agtttaaata
     3301 tgtaataatt ccagctgaag aagcaattcc tatttgggat agtaaaagac agagggaatt
     3361 agttgcattt attaggtttt attatattga agatatagat ggaaataaaa taaaaagagt
     3421 tgagtactac acagaaaatg acgtaactta ctttattgaa agaggtaata gttttattca
     3481 agaattttta tatgatgaat atggaaaaat gactgatata caagaaggtc attttagaat
     3541 aaataacaaa gaacagggat ggggtaaagt tccatttata ccttttaaaa ataatgaaaa
     3601 gtgtgtctca gatttaactt tctataaatc attaatagat atatatgaca ataatatttc
     3661 tacactagca gataacttag atgaaataca agaggttatt tatgtattaa aagaatatcc
     3721 aggaacaagt ctacaagagt ttatagataa tataagatac tataaatcaa ttaaagtaga
     3781 tggtggaggt ggagttgata aactagagat aaatatacca gttgaagcta aaaaggagct
     3841 tcttgataga ttggaaaaga atataattat ctttggtcaa ggagttaatc cagaatctca
     3901 aaacacaggt gacaaatcgg gtgtagcact taaattttta tattcactac ttgacttaaa
     3961 atgttctaag actgaaaaga agtttaaaaa agcaattaga gagcttttat ggtttgtgtg
     4021 tgagtattta aagataagtg gtaataagag ctatgattat aaaacagttc aaattacttt
     4081 taatcactct atgataataa atgaagctga aaagatagat atggcagcta aatcaactgg
     4141 aattgtatca gatgaaacta ttgtttctaa ccatccttgg gtcgaggatg ttaatgacga
     4201 acttgagaga cttaaaaaac aggaagatac tcaaaaagag tatgatgatt taattcctaa
     4261 taatcaagat ggtgttatag atgaaacata aagattattg gaggaagaga tttgaacaat
     4321 tagaagaagc tcagaataat aaaagtataa aatattatct tgaattagaa aaacaatata
     4381 aactagcaat gtctaatata gaaaaagata tacttatatg gtataacaga ttcactgaaa
     4441 atgagggaat atctttattg gaagctaaga aactgctaaa tacaagagaa ctagaagagt
     4501 ttaaatggag tgtagaagaa tatattaaat atggtaaaga aaatgctata aatcaaaagt
     4561 ggatgaaaga gttagaaaat gctagtgcaa gagttcatat aacaaggctt gaagctttaa
     4621 aactgcaaat acagcaacaa gtagaagttt tatatggaaa tgaacttgat ggtattgata
     4681 aactaatgag agatatttat acaagtggat actatcatac agcttttaat gttcaacaag
     4741 gagtaaacgt tggttggagt ttaatgagtc ttgatactaa cagaataaat aaagttattt
     4801 ctaagccttg gactagtgat ggattaaact ttagtgaaag aatttggggt aagcatagac
     4861 ctgctttaat caatgagtta catactaagc taactcaatc aattattaga ggtgaaaatc
     4921 caaaagtttt ggtaaatgaa ttttctaaga agtttaatgt atctaaatca caagctaaga
     4981 atttaataat gactgaatca gctttctttg catcagcttc aagaaaagat tgttttaatg
     5041 atttagatgt agaaaagtat gagattattg ccacactaga tttaaaaaca tcaaatatat
     5101 gcagagagtt agatggaaaa gtatttgata tgaaagatta tcaagttgga ataacagctc
     5161 caccatttca ttgtcattgc aggacaacaa cagctccttg gttcgaggat gaagaaggtt
     5221 atagagcagc aagagatgaa aatgggaaaa catattatgt gccatctggt atgaaatata
     5281 aagaatggta tgaaaagaca tttataactg ataaaaaaga tgatatacta agtaataaga
     5341 gttggttaaa agcagatttc ccaactgaaa agaaatttaa aaagcatgta gaaaaacact
     5401 taaatgaata tggaaatata actgctgaag aatatttatc tactgcaaga aatttgttat
     5461 cagagcaatt aagtaatgat gtagaaggat ttttaagtaa agataacttt ttatttaaat
     5521 atagaaagag tactaatgat tttgctgttg gaagagctga tggtaaaata tcaaccttat
     5581 ttaagccaaa agaaggttat aattactggc tagaacaaat aaaagaatat aaggaggttt
     5641 agacatgaat tgtccagtat gtggtaaaga tgtagatgta tttgatattt gtgataattg
     5701 taattggcaa aacagtggac caaaagaaac taattacaaa ggaccaaata aaatgtcatt
     5761 aaaagaagct aaagaggctt atgaaaaagg taaaaaagta atataaaagc acttgctaag
     5821 ttataaatta gtaggtgctt ttattatgta aaaatttatt gcgagggtga tttgaaatgc
     5881 ttaaattata tctatactta ttttatcaat aattgtgttt tgtacagggc tttatttatt
     5941 caacatgaag gttgatagta atgaggaatt aattgaatta cttaaaagta agaatatcag
     6001 aagaagaaaa aaatataatt ttattttccc agcgttattt ccactgctta attttatttt
     6061 aggtgtgata ctgatactat ttt
//
LOCUS       DAYAOY010000165         5758 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYAOY010000165 DAYAOY010000000
VERSION     DAYAOY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.169"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(83..985)
                     /locus_tag="ACGI6B_003800"
     CDS             complement(83..985)
                     /locus_tag="ACGI6B_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9753207.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(1035..1496)
                     /locus_tag="ACGI6B_003801"
     CDS             complement(1035..1496)
                     /locus_tag="ACGI6B_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HFG9753208.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1548..2003)
                     /gene="def"
                     /locus_tag="ACGI6B_003802"
     CDS             complement(1548..2003)
                     /gene="def"
                     /locus_tag="ACGI6B_003802"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFG9753209.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1975..2238)
                     /locus_tag="ACGI6B_003803"
     CDS             complement(1975..2238)
                     /locus_tag="ACGI6B_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9753210.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2335..3051)
                     /locus_tag="ACGI6B_003804"
     CDS             complement(2335..3051)
                     /locus_tag="ACGI6B_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753211.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(3048..3731)
                     /locus_tag="ACGI6B_003805"
     CDS             complement(3048..3731)
                     /locus_tag="ACGI6B_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753212.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(3728..4573)
                     /locus_tag="ACGI6B_003806"
     CDS             complement(3728..4573)
                     /locus_tag="ACGI6B_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753213.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4687..5277)
                     /locus_tag="ACGI6B_003807"
     CDS             complement(4687..5277)
                     /locus_tag="ACGI6B_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9753214.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            5566..>5758
                     /locus_tag="ACGI6B_003808"
     CDS             5566..>5758
                     /locus_tag="ACGI6B_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFG9753215.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTL"
BASE COUNT         2036 a          900 c          551 g         2271 t
ORIGIN      
        1 tatttaaatt ttatatttgt caataaattt taattttaat aaattttaat catcatagta
       61 aaaaaaattt ttttatataa aactaatcaa aaaaagatat ctgtacatct cctgagtttt
      121 ttaacttttc cttcattatt ttagagtaac tgcttgatag agaatattta tatctaagtt
      181 cattaaccat tttatacaat tgatttttat actccctatt aagtgaacct tcactatata
      241 gtattgataa tttatcaaat aattcaggaa actctttttt aacaaagtca aaaaaaacac
      301 ctctagttat accccttaag tataaagtcc caggtaaaac ataatgtaca ttacattctt
      361 ttgcatgtct aaatagcgaa tttatattat caaaatcatc tgtaatatat ggtataattg
      421 gcataatatg tagaccaact gatgcgttag tttttctaaa ttcttttaac attttaaatc
      481 gtctcattga atccacacca tttggctcaa taagcctttg tgtatcttca tttactgtgg
      541 taattgtaga tgctacattg atatatgtaa tcctagatag cttatcaatt aaatcgtaat
      601 ctcttaaaat taaatctgac tttgtagaaa taatagctgg tgtcttgtat tttattagca
      661 ggtttaatat ttctggcata attttatagt cagcttctat tggttgataa ctatctgtaa
      721 ctcctcctat attgattact tctttcttcc atttactgct tttaagttgt ctctcaagtt
      781 tttctactat atttgttttt acataaatct cttcaaaata attattggag ttgatgtact
      841 tatgagagta aatagcataa caatatttac atccatgctc acatccacga taaatattta
      901 aatcccattt atatggcata ctccttttta atttattaca agctatttca caatttattg
      961 gaatatattt tattttatta tacaaataat cacctcttta tatacaataa tactataatg
     1021 ttaatctata gtaattaaaa catgatattt ccagaccaca attcataatc tttactatat
     1081 ttacaaagta atatattcca ttcatttatt ggtattcctc cacatgttgc tttttcttct
     1141 aaattatata agcattgctt taaatacatt ttattatgcc aatcatcata taaattttct
     1201 aaatttaaac tatgttcatt agatatatca tttgcaaaat ttctattttt ccattttaat
     1261 atacttgtat atttaagttc attaggttta taatttcttt ttaacatttc atcatgtaca
     1321 atttttgtat aaaatttaaa ttcatctata ctataattca atactttatt cactaataga
     1381 tggtttggag tacctttttt atctatagaa ccttttatag caagtagctc tctccattga
     1441 cttactaatt gatttttagg taatacatca attaaatcct tatgccataa tctcataatt
     1501 taactcctta ctataaaata ttttataaat tcaataacca atctatctta caattttaca
     1561 tgttcaataa ttttatctgt aaaaactata ccatttaaat ggtctatctc atgacaaaga
     1621 cattttgcca taaaacctga acctttaatt tctattttct caccgtattc gtttagagct
     1681 tgcactgtaa ctttttttgg tctttttaat ttaccccata cttcaggaaa acttaaacat
     1741 ccttcaacaa caatttgttc cccttcttgt ttaataattt caggattaac aagcttaata
     1801 agtccttctc ctaaatctat aacaactaat cgctttaata ctcctacttg gcatgcagct
     1861 aatcctcctc catttggagt attgtacatg gtctctgcca tgtcatttaa tatttctctt
     1921 atcttattat ctacgttttc tacatactta ctcttttttc ttaaaatttc atcatcaaat
     1981 gttcttatct ttcttatagc cataatattc tcccctactt atctgtgctc ttgattttct
     2041 ttcactttct tcaaataaaa atacactttc aattggttca ttaaaaattc ttgaaatatc
     2101 atatgccaac catattgaag gttcattttt accagtttct atagcattta tagcctgtct
     2161 agatgttcca actaatctcc ctagttgttc ttgtgtcaat cctaattttt ctcttaatat
     2221 ctttatttta tttttcatat ttgctccatt catgtaaggt taactttaca tcttaaatgt
     2281 aacatttttc aacattactg tcaagtcaac cttacattta tatattaaat tttcttattt
     2341 aactattttt ttactaaact ttttaaataa taataaaatc catattaatg atataacaat
     2401 tgaaataaac atatagacat aattatcttc cttaaatgcc ttcgatatcc aaaaagttgg
     2461 taagaagaac agaatatatt gtgctttatt taaaataaaa aatggtgcta aaatacccat
     2521 cataaaaaca cctgagaatt tcgtaattac cattccttct agtttatttg atgataaaga
     2581 aatcactaat attgatataa taattccttg taataaaact aataaagaaa ttgataagtt
     2641 taacaagaaa gaaattttag ttagagaaaa aaagataagt agaatcatag taataataaa
     2701 tgctaagata cctggtattc caaatcgtga aataagataa ccagttttac caagcggagt
     2761 tactattaaa tatcttgaaa tgctgtcatc aatttctccc aatatgacca ttgtagaagc
     2821 aaaaaagaac ataaaaggtg taataaaagc taaaagtaaa tcaaacatca agaaatacgg
     2881 ctctaaattt aattgataat aaaacttgtt aagtgatata ttctgtatta atggtattcc
     2941 aaattttata aatactccac ataatattgg agaaacacat actataaaaa gcatagcatc
     3001 tctcttaatt tgttcaaact cttgtttaaa cgcatttata atctttatca tagttttact
     3061 ccacctacct ttttccaact gctacaaata aaataataag ttataaaata aattattatt
     3121 ataattgata ttaaaattaa aatattaatc attataaaat ttttatctcc actaataaga
     3181 gaaatacata tattaaaagg gtacaaattt gcatatgatt ttgtgtcaag aagtacattg
     3241 agtattggtg gaataaagca tataatctca ataggaatac ttaaaacaat aaactgattc
     3301 aaactagatg ctttacttgc aagtatcaat ccaagcaaag aaaatataat agaagaaaaa
     3361 aaggtaccta tagttgatat tataatatta tctaaattta atgtaatagc aataaacata
     3421 gctacaatac tagatattac tcctaatgaa attacctttg ataaaatata ctcagatact
     3481 tttactggag atacagctaa ggaatttaaa accctttgac ttttctcaag aagtacaata
     3541 gctcccataa aaaacaatcc cattgctgct gggtcagaat aaatcataat tatagctatt
     3601 ttttctctca taaaagttgg aaatacattg ataatacaaa tatatataat acttaaaaaa
     3661 agatataaaa aataaaaacc atacttaaat tgaaacttta tatctcctaa aataagattt
     3721 tttagcctca ttgtaaattc ctccctgtta tctcaataaa aatatcattt aatgttggtt
     3781 cactactatg tattgataaa agtttatttt tctcaataag catattcaaa tttttatcat
     3841 tagcagtatt gttcagaaaa cattcagaag ttttttctcc attatcaaag taagtatatc
     3901 taactttaat agctcctttt gacataataa gattatgtgg tgtatctaaa gcagatattt
     3961 taccatttac aatgaacgct actctgtcac aaagctctgt tgcatctaac atgttatgag
     4021 ttgttaaaat aatagtttta cctttagatt tttctgataa aattatatct ttcataacct
     4081 tgctatttga tgggtcaaga ccacttgttg gttcatctaa aaataaaata tctggattat
     4141 gtaataaagc ttttataaaa tttaatcgag atttcatacc ttttgaatac tcagataccc
     4201 ttttatttgc ttcattttct agtcctactg attttaataa ctcatcaata gatagtagtt
     4261 ttttagaata tagagaccca aaatatttta aattttctat tgcagtcaac ttttcatata
     4321 aactaggaaa ttcaaaatca actcctatat tttcataaaa tttatttgag tgtcttttac
     4381 tctctactcc atttacaatg acacttcctc cataattagt tatcattcca attagaatct
     4441 tttgtaatgt agattttcct gcacctgatg gtcctaaaaa tcctagtatt tccccttttc
     4501 ctactccaaa gttaatattt tgtaaaaaat ctctctctgt ataactaaat gataaatcat
     4561 caactttaat cattcaatat caccctttca aatttttgac tattttgact atatggtcaa
     4621 taaaatataa tttttttgtt tgttaataat ttttaataaa agttattagc aaacaaaatt
     4681 taaattctat tccataagtt gtataactaa tccgtttatc attaatctta aagcatcttc
     4741 aaaacaatta cttccaacct cgcgcttata tagcatggta agaaaaactg ctctaaatgc
     4801 tccactaaag ctttctatgt ctttattttt tgcatttggt atgcaagata taatctctcc
     4861 aatttcaaag tcatcatgtc taaagtgttc ttcaattata gaatctggta actttctcac
     4921 caatatctca aattctccat ttatcataag atttaaaatt gaagtactat caaccatcct
     4981 atagaatttc ataaatatat ctgtcaattg ttcacatgta atactttcat ctactctatt
     5041 tatctcatat aagatttctt tttgtataga gtcatgtata tctcttaata cctcaaaaaa
     5101 gagcaattct tttgattgaa aaaatagata aaaagttcct tttggtattt ttacccgttt
     5161 tacaagctca tcaacagtag tttttctaat accatattgc tccatacaca atcttgcttc
     5221 ttctttaagt ctttttatta tatactctct ttctttatca gaatacgatt taggcatctt
     5281 ttccctccag tttgactaaa ataatttata tagtcaatat aatacttttt ctatctaatg
     5341 tcaatagtat tattagtata tttaaaaata agcttaaaaa ctttcgaaaa ttctgaattt
     5401 tatatagcat tttttattat ctagtgttat aattaagata taaataatat attattctaa
     5461 ttttcatatc tatttgctta ttttttaact tagtttctaa tagaagtaat catatttatc
     5521 tttttaaaag tttcaacttt aataaaaaat aaaggagttg attttatggc tactactaca
     5581 aaaaaccgtt tttacgatta tgttcaagaa aacttaaata aatatgttga agtacattct
     5641 tactttagca gttgtcctct atatggtgaa attatagaag cagatagctt atcaattaca
     5701 ctttgctcaa gatatataga tgaatctaat ccaactttaa atgaaactta cacattat
//
LOCUS       DAYAOY010000166         5648 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYAOY010000166 DAYAOY010000000
VERSION     DAYAOY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.170"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            226..429
                     /locus_tag="ACGI6B_003809"
     CDS             226..429
                     /locus_tag="ACGI6B_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9753216.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            620..1156
                     /locus_tag="ACGI6B_003810"
     CDS             620..1156
                     /locus_tag="ACGI6B_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HFG9753217.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(1160..2026)
                     /locus_tag="ACGI6B_003811"
     CDS             complement(1160..2026)
                     /locus_tag="ACGI6B_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HFG9753218.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            2174..2419
                     /locus_tag="ACGI6B_003812"
     CDS             2174..2419
                     /locus_tag="ACGI6B_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFG9753219.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(2533..3831)
                     /locus_tag="ACGI6B_003813"
     CDS             complement(2533..3831)
                     /locus_tag="ACGI6B_003813"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFG9753220.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3964..4977)
                     /locus_tag="ACGI6B_003814"
     CDS             complement(3964..4977)
                     /locus_tag="ACGI6B_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFG9753221.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
BASE COUNT         1944 a          863 c          585 g         2256 t
ORIGIN      
        1 actgtaatat ttaatttata agacaataac taaaaaaata aaacctatat tgacctaatt
       61 aaactctcta aaatactcat ttatttaatt tttttatatt ttttacattt ttacctttat
      121 ttttctgtat atattatttt tattttggag aaattagtat taacatcaca taaataaaag
      181 tgttgttaga catcataaac taattatttg aggaggaaat ttactatgtc aaattcaaac
      241 agaacagtag ttccagaagc aaaagctgct ttaaaccaaa tgaaattaga aatagctaac
      301 gaaataggtt tatctaacta tgaaaacata gacaaaggta acttaacagc tagagaaaat
      361 ggatatgttg gtggatatat gactaagaaa ttagttgaaa tggctgaaag acaaatggct
      421 ggaaaataaa tagtaaattt tgacacctgg gtatcaacag ggctgctaat tagcagccct
      481 gtttttctat atttatttat aaaattgttt ttatatgaat tattaaagat taataataca
      541 atacaaattg ttcttttaat tttgtacaaa ttagtttaat atattatata tagaaatagt
      601 ttgacggagg atgatattta tgtttgaaaa ctcgtctgaa gaattagcct atcacaaact
      661 tttaatttta tatatattag aaaaaattaa aatggattta actaattctc aaataactca
      721 agtagtatta gagactgaga tgatgaatta tttttcatta caacagcttt tatctcagct
      781 tatggaatct aagtttttga ctacatataa agactctgac agagaatatt atgctcttac
      841 tcaaagaggc gtagaaagtc tagaatactt ctttaatcga atacctacta gtgttactga
      901 aaaaatagac aagtatatag attctaacaa agaaaatcta ctagcagata ctcaagtaaa
      961 gtctagtttt gtaaaacaaa gtgataatga atttattgtc aaccttagag tcatagaaaa
     1021 tcagtctaac ttaatagatt taaatctaaa tgtttcatca gagaaacaag caaaacttat
     1081 ttgcaataac tggaaaaata atgcatctta tatgtatgcc gaagttatcg atttacttat
     1141 aagagacata cattaaagcc tacaaacaaa ttatagttgt aggctctttt cctaccttga
     1201 tttcttttat taatttgtta tttgcagttt cataaacttt tactgaattt tcttgtgtat
     1261 ctgatacaaa tagtaacttt tcatctttag atatttctaa tccttgggga atcccattta
     1321 tttcaatatt tccaatgtat ctactcgttg tataatctaa aattcttaca ttattataac
     1381 ccaaatcagc aacataaact tttttgccac tataattaaa tcttatttct actggtaata
     1441 aaaattcata taataccttt tttatatcca tgcttacttc atctattatt ataagctttc
     1501 catcaagtgt tgatatatac atttctttct tttgtttatc aagttctata tgccaagctc
     1561 taaagtccat atttattttc ttttgtatat ttttattaat tgtatctata catactatcg
     1621 agtttataca cggtacatat aactttttgc tttctctatc aaagtcaaat ccatgtggca
     1681 tattatcaac tccaataatt cctattggat ttaatgtgtt tttatctagt acatatatac
     1741 tattagaatc ttcatttgcc acaaacaatt cattatcgct aagtacaact tgactaagac
     1801 ttcctcctat agagacagta tcaattattt tatcattact taaatctaag acatagagta
     1861 tcccattact tgaactaggt atgtatatta aatttttttc tttttctatg caaaaatggt
     1921 gtggatatat attatcttct aatactattt ctttttctag ttcaagtgta gaataatcaa
     1981 ctatgctaat actctttgat aagtagttag aaatatagat tttcatataa cccctcctct
     2041 ctacaataca tatactacaa taatatatta tttcattagc ttgtattatg ctttatagtt
     2101 gcattattac gcatatattt ggtattatta ttaatgttaa aaaaagaaat aatatttaat
     2161 ggaggtcttt ttaatgaaag taacatttaa tccaagtggt gtatgttgta gagaaatgat
     2221 ttttgaagtt gatgaaaata atgtgattgt tgatgctgag tttataggag gatgtaatgg
     2281 taatttatca ggacttagaa gcttaataat tggccaaaat gctcttgaag ttgctgataa
     2341 attaaatggt attgactgtg gtggaaaagg tacttcttgc ccagaccaat tatctaaagc
     2401 tatacgtcaa tctatataat tacttatata aacatcagta aaattattaa aaacttattt
     2461 tatataaaaa gagttgtcca ttttttagac aactcttttt tatattttta tttgtatact
     2521 tattttataa aattataatt tctttactaa agttctcact gtaccaatca tatataatga
     2581 acctgcactt attataatat catcctcact tgatgtttct aatgtataat ttactgcatc
     2641 ctctatctca tgctttgatg ttacatcatc tacatatttt aatacttttt ctcttaatat
     2701 atctgaattt atggcccttg ggttatttgg tgttgttgtt attaccttat taaaatgtga
     2761 caacaatatc tctaaaacac tatcaatatc tttatcttct aacataccta ttaataaggt
     2821 taattttcta cctttaaaat tcttatcaag tgcttttgcc aaagattttg ctccatcttc
     2881 attatgtgca ccatctatta taaaaattgg attttccttt atcttttcta ttcttcctgg
     2941 ccatttagta tttataagtc cttttcttat agattcttca tttatatttg ctaatttatt
     3001 tatgtctttt agatatttta ttacactaat tgccaagata gaattgttta tttgatgttc
     3061 gcctatcaat tttatttcaa ggtctctata agtttctttc attacagtac aatcgtatat
     3121 ttgagaattt atatctgatt tttttatgtt tatatcatca aaatcaactt ctatatattt
     3181 agcttttttc tctttacaaa ctgactttat aacatccttt gcttcatcag tctggtcata
     3241 tactagtact actccatttt cctttattat tccagccttt tcgtacgcta ttttttctat
     3301 tgtatctcca agtatacctg tatggtccaa acttatagat actatcacac ttacaagtga
     3361 ttttgttatt atatttgtag catcatatct gccacctaaa ccaacttcta atgccacaaa
     3421 gtctaccttt tgttctgaat agtaataaaa tgccattgca gtaaccactt caaattctgt
     3481 cggatatgca tagccttctt ttaccataat ctctattttt tcttttatta actcaattat
     3541 tctagcaaca tcatcctgtg gtatgttttg tccatttact cttatacgct cagtaaaagt
     3601 ttctaaatat ggagatgtat ataaaccaac tttatatcca cactctctta gtatattaga
     3661 aacaaaagaa cacacagaac cttttccatt agttccagct acatgtatta tattcaaagt
     3721 ttcttgtgga tttcctaata attctaataa ctttccgata ttttctaatc ctaatcttat
     3781 accgaattta ttagtttgtg atatgtactc taatgcttct tcatatttca ttatctaact
     3841 ccccctgtat tggcattttc tcttatgcta tctaattata gtatattttt tacttttaat
     3901 ctaccgacaa agaatacata taatttaata ccttctacat taaatttagc tacaaatctt
     3961 tatttataag tttttatatc tttccactaa ttcagtcagt gtatatattg aaactaagtc
     4021 atattttgag atgtccatat aattaaactt tatgtcaaat ctatgctcta gctttttcaa
     4081 cagattaaaa caattatcat aaaccaagcc tatctcctta ttaaaaaggc tacccttaaa
     4141 gagtttttca ttattctctt caatatctaa gcaatcccta acaacgtcag caactttcat
     4201 aaaaagagca ttttctttgt catacaaatc caatttaaag tttacaattc tactacactc
     4261 atcaaaatca caactatccc aatgatttct agttgctaat gattcaagct tatcatttaa
     4321 atcagatatt gttatgcctg gtttttctga taatatctta gctattttac cagtaagctt
     4381 tgctgataaa taactattac attttggtgg taatctatag gaattattta tatcacaact
     4441 tatatatgaa ttgctatcca taacacattg aacatcatat tttttgttgt accaaaatga
     4501 atcatctcca tctaaagtaa atcctcttac acctataaca ttgttaaaca tagctggata
     4561 acttaagtca aaatcattag cgagtgaaca tactattatt tttccttttc taaacaattc
     4621 atagcaaact tcaaataagc ccttaacact ttctactcct atgactgata aacttaaatt
     4681 aattaatctt atgttagtgt ctaaaaggta tcttaatgct tcttcaagaa cttttatatt
     4741 tgtaatacca tcttttctca aaatttttat tacaaataac tctacatctt catactcttt
     4801 ctttattatt gatgcacaag cagttccatg accatcttca tcatcaaatt tatctgatat
     4861 aaagatataa tcatgcatac attcaaaagc aattcctcca actaaattat cttttaagta
     4921 atcatgttct ttgtctattc ctgtatctaa tactgcaacc ttaactttat tattcatatc
     4981 acatatattc tccattacaa aagtttttaa tatatctatg ttaatagttc atcttaaact
     5041 ttgatttaag ttgcacaatt tatcttttaa atgctatatt tcttgctaaa aacataaaat
     5101 aagtacacca tatatttaat ttatatttat tatatcaatt attatcatta aatggttaaa
     5161 tgttaagata aaatttttag atttatatca caatttagaa ttatttgtaa aaaaggagat
     5221 tatcttttta ttagacaatc cccttgtata ttttattaat atttatctat gattaagcat
     5281 caattttatt ttatactttt tgattttaat actatacatc taaatcttat ttagggggaa
     5341 caattattta tatgatataa aaatgctaat ttgaatgaac ttgtcttata atcagtttta
     5401 gaaaatacac cattctacaa agtatgaatc aaaaggtgaa aaaatactaa aaataataaa
     5461 tcaacatcaa ttttaatcaa tatatgaaaa acttatacca agtatgtata aaaactaata
     5521 taaatattat tttaacaact aatacttaca cctttatata ttatatcata tttcaataaa
     5581 taaattgcat aaaaggaaat cttttcatag aattaaacta tttattaatt cattactata
     5641 gtttcaat
//
LOCUS       DAYAOY010000167         5625 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYAOY010000167 DAYAOY010000000
VERSION     DAYAOY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.171"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(137..820)
                     /locus_tag="ACGI6B_003815"
     CDS             complement(137..820)
                     /locus_tag="ACGI6B_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFG9753222.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1189..1779)
                     /gene="zapA"
                     /locus_tag="ACGI6B_003816"
     CDS             complement(1189..1779)
                     /gene="zapA"
                     /locus_tag="ACGI6B_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFG9753223.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1804..4197)
                     /gene="pheT"
                     /locus_tag="ACGI6B_003817"
     CDS             complement(1804..4197)
                     /gene="pheT"
                     /locus_tag="ACGI6B_003817"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFG9753224.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4214..5233)
                     /gene="pheS"
                     /locus_tag="ACGI6B_003818"
     CDS             complement(4214..5233)
                     /gene="pheS"
                     /locus_tag="ACGI6B_003818"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFG9753225.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5283..5517)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1718 a         1084 c          601 g         2222 t
ORIGIN      
        1 ttactatact taaaatactt ctattaactt atacattgct tactatattt taaattcttt
       61 aattaatcta tacaatattt gctatatata aagtatatca aataacagct tcattttttt
      121 gtaaatttac agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc
      181 caaagaccac tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg
      241 taaaaaaagt tcctaaaata gctccagata taaagaatat aataatagca taatactgta
      301 aactcttttt tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag
      361 cacttcttaa atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa
      421 aactctctac ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc
      481 ttcctctagg aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct
      541 gacgccaatg aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa
      601 atacacctaa cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac
      661 cttctgctat attaagccca aataaaacta tatttccagt ttgtgcatta gcaaagactt
      721 tacctcttac cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac
      781 ataaaaagaa tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct
      841 aatgatagat acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt
      901 atttatattt aaatattgtc tatatttata cattatttgt atttatatag cattcatatt
      961 tatttatatt taaacattat ctatatctat atagcattta tatttattta catttaaaca
     1021 ttatctatat ttatatagca tttatattta tttacattta aacattatct atatttatat
     1081 agcatttata tttatttaca tttaaacatt atctatattt atatagcatt tatatttata
     1141 cattaaaaga agctgtctca aaaaacaaga gacagcaaat cttttagttt attccacatt
     1201 ttttgcatca ttatttaacc ctgtacattt taactgtaaa tcataagctt tgttttgaaa
     1261 ctcagaagct aagttttcag ctatagtagc tctttcattt gcttcttcaa gttgagtact
     1321 taactcctca atcttgtttt tataagcatc taactctgct ctagaacctt ctgtcgtatt
     1381 actaagttcg aatatttctt gctttttatt ttctataatc tcaattaact ctttcatctt
     1441 agtttcagct tcacgatttt ctgcttcttt ttctgctatc gtaagcttta aagatttgat
     1501 ttctaattga agctcttcat tagatgctcc aaccttctta ctcaattctt cattcgcttt
     1561 aatcaacttc tcattctcat ctgaacattc aaataaaagg tctgatatat ttaatgcagt
     1621 taatatcgca gcaactgata agctcaattt cgggttttga cgtgttattt tatccatttc
     1681 tttgtctaca aaatcagcaa tactaatcat aaacttctct gatttgtctc caaccattgg
     1741 atattcagcg ccatggattt taaccattac tttgttcatc ctaacctccc ccgacattag
     1801 ttgttaattt gatcttaagt tagcatttaa tttttcgctt aattcactta atatcttatc
     1861 atgcacctta gctacatctt catctgttag agttttatct ttacttctat aagttattga
     1921 atatgcaatt gacttgtatc cagcttctat ttgagaacct ttgtaaacat caaataactt
     1981 gtagctttct actaatcctt gaccattttc ctttattata tcttctattt gttttacaaa
     2041 tacatcatct ttaacaataa gagctatatc tcttgatgta gatggatatt ttggtaatgg
     2101 cacataattc tttgttctat cagaattatc aaatacaaag tcaatatcta tttcagcgac
     2161 ataaactctt tgacctagat tgtagttttc tataacatct ggatgcaatt cacctaatgt
     2221 acctacatat ttattattat acactatttt agcacatcta ccagggtgga aggtagtatt
     2281 attatcttga ggttcaattt cataaccttt aaatccgaca tttacaagta tagtttctat
     2341 agttcctttt agtgcaaaga agtcaacatc tttaccatac atacctacac acattctatt
     2401 ttcttcctta ggtaattctg aatcttgtgg gatgaatata tgtccacact caaatgcaga
     2461 tacttcttct attttatgag atacattagt tgataaaaca tctaacatat ttggtattaa
     2521 agtagttctc attacagaag tttcttctcc taatgggtta agtaatttaa caaaatttct
     2581 ctttgcatta ccttctggta ctcttatctt atctactccc ttagggctta caaatgaata
     2641 tgttaatatt tcatttaatc ctacagcagt tgaattatct tttaaagcat ccatgaattt
     2701 ttgttttgaa gttttaattc ctgctgtcgt atttccttct aattgtacag aaggtatatt
     2761 ctcaaatcca taaattctag ctatttcttc ccatacatca gcttcttgtt ccatatctgt
     2821 tctaaagctt ggtacatcta tttctaattt atcattagct accaaattac atttaaactc
     2881 taatgattct aaaattccta caaactgctc cattggtaca tctacaccta ataggtggtt
     2941 aattctttga ggatttacta ccaatttttt aggttctggt ttatttggat atacatctac
     3001 aacacctttt aaaacttttc ctgctcctaa catttcaaca agttgtgcag ctctatttgc
     3061 tgctatctct gcaagattag ggtctaagtc tttttcattt cttgaagatg cttctgacct
     3121 aataccaact tttttagctg tcattcttat gttttctggt ttgaaattgg cactttcaaa
     3181 aagtacagtc ttcgtattag aagttatttc tgagttagca ccacccatta caccagctaa
     3241 accaagtgat ttttctccat ttgttataac tagcatatct ttgtctaatg ttctctcaac
     3301 atcatctaat gttacaagtt tctctccatc tttagcattt cttactacta ttcttccagt
     3361 ctctacttga tttatatcaa aagcatgaag tggttgacca agctctaaca ttacgaagtt
     3421 tgttatatcg actatgttac ttataggtct tactcctgct tctgtaagtc ttctttgcat
     3481 ccaatatgga gaaggttcta tttttacatc tgtaaccatt ctagcaacat atcttctaca
     3541 taaatctgga ttatctatct caactttgaa atctatctct tcgtcacttt cttttacttc
     3601 gatttcagga tattttactt ttgttcctat agttgcagct gcttctctag ctatacccat
     3661 catgcattta caatcaggtc tgtttgaagt taattcaaaa tctattaaag catcttttaa
     3721 tcctaaaaca tcttttatat cttttcctaa ttcataagaa tcttcgtggt ctaaaatata
     3781 tataccacct cttttatact cctcaatgta atgttcatct atacctagtt cagctgctga
     3841 acacatcata ccatctgata attcacctct gaaatcagtc tgtttgattt caactcctcc
     3901 aggtaactta gaaccattta cagctactgg aatataatct ccttctgata tatttgtagc
     3961 tcctgtaact atttgaacaa ctttatctcc aatatctact ttagttacaa ccaacttatc
     4021 agcatttgga tgttgtttta tttctaaaat ctttccaacc aatatatttt ctatttcttc
     4081 accataataa tctattgttt caactttagt tcctgtcatt gtcattttat cagcgaactc
     4141 ttttacatcc atgtctatat caacatagtc tctaagccat tttaaagata ctaacatcta
     4201 cataccctcc taattaaaat tgatttaaga atctcatatc attttcgaat aataatctaa
     4261 tatcatctat ttcgtattta agcattgcaa gtctttcaac cccaactcca aatgcaaatc
     4321 cactgtaaac ttctgggtct attccacaat ttctaagcac atttggatga accatacctg
     4381 aacctaatat ttctatccaa ccttcatatt tacacattgg gcaaccttta ccaccacatt
     4441 tgaaacaagt aacatcaacc tctgcacttg gttctgtaaa tgggaagttg tgaggtctaa
     4501 acttagtttt gatatcagaa ccaaacaatt tttcaacgaa gatatccata gttcctttaa
     4561 attctgccat agtaacatct tttccaacaa caagcccttc tatctgatgg aacattggtg
     4621 agtgtgtagc atctggcgag tctgacctaa aacatctacc tggtgcaatt acttttattg
     4681 gtaactcttg acttctcata gttcttactt gaactggaga tgtttgagtt ctaagtaata
     4741 ccccatcatt gatatagaat gtatcgctca tatctcttga tggatggtct ttaggagcgt
     4801 ttaatgcgtc aaagttgttt tcaacagtct caacttctgg cccttctgct atagagaatc
     4861 ccattccgat aaatatatct gttacttcat ctataatttg agttattgga tgcttcttcc
     4921 caacctttaa aacttttcct ggttgagtaa catctatcac ttcctctttt aattttcttt
     4981 ctttttcaat agcatttatt tcttcttttt tagaatttat gctaagttca atgttttctc
     5041 ttacctcatt ggcaacctta ccaactactg gtctttcttc agcagataat ttacccattt
     5101 ctttaagtat ggcagttatc tcaccttttt ttcctaagta cttaactctt aaactttcta
     5161 cactttctat gctttgtgct tcttttattt cagccaaagc tgcttcacgt aaagcaagta
     5221 atttttcttg cacacatatc accctttcgt ttttttgtat aaaaaaagcc cctcaatccc
     5281 tgtctaggga cgaaaggcta tagtttcgcg gtaccaccct agtagtttaa caatttaaaa
     5341 atcatcaaac cacctctaac aaacttaacg cgtttttgac gtcaaagcct actgtaaaat
     5401 ttcagctctg ttactccaga gggaacttct catgacctct tagaaaacac tcccagccta
     5461 ggtgtttatc tctttactaa gtttaatcaa aatactttcc tctttcattg catttacaat
     5521 attatattgt ttattttagt attatatcat ttccattcaa attatgtgac tatttttaat
     5581 atatatccaa tacataatta atttaattat tttttatata atata
//
LOCUS       DAYAOY010000168         5583 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYAOY010000168 DAYAOY010000000
VERSION     DAYAOY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.172"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            269..766
                     /locus_tag="ACGI6B_003819"
     CDS             269..766
                     /locus_tag="ACGI6B_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFG9753226.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(942..1190)
                     /locus_tag="ACGI6B_003820"
     CDS             complement(942..1190)
                     /locus_tag="ACGI6B_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753227.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(1256..2272)
                     /locus_tag="ACGI6B_003821"
     CDS             complement(1256..2272)
                     /locus_tag="ACGI6B_003821"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFG9753228.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(2330..3097)
                     /locus_tag="ACGI6B_003822"
     CDS             complement(2330..3097)
                     /locus_tag="ACGI6B_003822"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFG9753229.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(3165..5354)
                     /locus_tag="ACGI6B_003823"
     CDS             complement(3165..5354)
                     /locus_tag="ACGI6B_003823"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HFG9753230.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         1750 a         1046 c          539 g         2248 t
ORIGIN      
        1 actaagagat tttgctaaaa actaataatt attaaatata attagtatct atttttatat
       61 aattgtaaaa gaatatttca acatattaaa ttattttatc ttatattttt ctctttagtt
      121 tcacacatca ccaagtctaa actctatatt atatattaat atttttgact tgtcaagtta
      181 tttatgttat aatattaatc attagataat taaagcattg tacctaagtt gcaatatcta
      241 aaattcaatt tggaggtata actagcatat gtataaaatt ggtgaaagtg taatgtatcc
      301 aaaagaggga gcctgctctg taaatgatat tgtaacaaag aaaataaatc atgagatgca
      361 aaaatactat gaattaagtg tcatttttaa tagtaacctt aaaatttcga ttccagttct
      421 aaatgcagat agaattggca tacgtccagt tatggatgga aatgatgttg ataattttat
      481 ccaatctatt aataaaacag atggtgtatg gatttttgat agaaaagaaa gacttaaact
      541 gtaccaagat aaatttcatt caggagatgt ttttgaaatt gtaaaattaa tcaaaatgct
      601 tatgatacaa gacagttcaa aacaattgtg tagcacagat aaggagtttt taaataaagc
      661 tcaaaaattt gctttatcag aattagctgc tgctcaatgt aaatcctata caatggtatt
      721 agaggaaatg aaaaaacata tattaaattc aaagaataca aattaaacca gttttttaaa
      781 ctggttttat tttttatact aatcatccat gctactttag cttattttaa actgcctaat
      841 ctaatattaa taaagttatc tcaattagat agatgtattt tattcaaata aaaaaaacta
      901 agagtaaatc atattactga tttactctta gctaataaaa tttaatcttc ttcaagttct
      961 tttttctctt gtaataatac acttaattca tttgcttcta aaacttgtgc ctctgcaata
     1021 tcaagtaact cttgttgcca ttccacacat ttatctttgt aatcaaaagg tagcgttctg
     1081 tcaactatgt acttatcaat acagtcctcc gcacttttaa ttatttcttt tctttcatca
     1141 ataaattttt ctaattctac taaggcttct tcataagctt ttctactcat ataaattccc
     1201 cctttgtaca attattctac attaaaatga tttttcctct tttttgtata aaattttatt
     1261 tgtatataac ttttccatca atatctttta tagtttgaca tccagttaat atcatagaac
     1321 tacttagctc tgattgaagt ttatttacat aagtttctac accatcagtt gcaccaccaa
     1381 aagaagcagt cacaaaaggt cttcctatta atactgcatc tgcacctaaa cctatcattt
     1441 ttagtacgtc taaaccagtt ctcactccac catctgcaag tattgtaact ttacctttaa
     1501 cagcatcagc aatttttggt aaaacttcac atgctcctgg tgtacagtca agcactcttc
     1561 ctccatgatt tgatacgact attgcatcca cacctgcttc tactgccatt aaagcatctt
     1621 caacagtcat tatacctttc aatataaatg gtagtttagt agactttact aactccttta
     1681 tttgtttaac atttttagga agtactggtt ttccatgtaa tgataaagtt actagaccac
     1741 aggcatctat atcaactcca actgcaaatg caccagcttc ttcagctaat cttatctttt
     1801 ttataatatt ttcattatcc caaggtttaa taaatacaat tccagctcca ttatactttt
     1861 tgactacatc taaattttcc attaaaaaag catctactgc cgtatcacca accattgcat
     1921 aaataccact attaacacat cctgcaacta ctggttctat gtaatctctt tcattaattt
     1981 ttccacccat atttaaagta gttcctgtta ctggagctgc aaatataggt gcactcattt
     2041 tctttccaaa catctctata gatgtatcag gatttgatac atcatgtatt actctcatat
     2101 ttacttttac tttttctaaa ctcttgctat tttctataaa agaagaacca cttcctttac
     2161 ctcccatgcc aggcacttca cctgcacaag caaccccatt acaaacctta caaactttac
     2221 aacttccatt taaattttct ctagcacttt ttaataattc attatattcc atctttaatc
     2281 ccccattaac tatagtaatt tatattatat acattatatc atattatatt tacagtatat
     2341 ttttcaaaaa actttttctg tggataggag ttatgccata tttttcaata gcttcatagt
     2401 gttctttagt tccatatcct ttgtgagatt taaacccata ttctgggtat actctatcat
     2461 attggtacat tatactatcc ctagtaactt tagcaagtat acttgctgct gctattgata
     2521 tagattttga atctcctttc acaattggat tttgagatat gtcaatacct ggtattgttg
     2581 ctgcatcaac taataaataa tccggtgctt tttttaaaca atttatagcc tttttcattg
     2641 ccatatatgt agcatttaat atattaaatt catctatttc ttcattgttt actataccta
     2701 ttccataatc tagtgcttct tcttttatta cttcaaaaag ttcatctctt tttgcctcac
     2761 tcagtttctt agagtcattt acaccttcta ttttggtatc ttttttaaat actacaacag
     2821 aagctacaac aggtccagca agtggtcctc ttcccgcctc atcaattcct ccaatatata
     2881 aataaccttt atcatagcct tcgttctcaa atatatttat agtttccaat ctttcttctt
     2941 cttttctaat attatccagc ttttttgcca gttttatagc tagaccttgt acagattttc
     3001 tttcatcaac tcttaacagc tctatatatt ccatatattt ttctacttct aaagtctcta
     3061 ttatttcttt aatctccctc acacttttat cctgcatacc attccctcat ctttcgtttt
     3121 taaaaattta atttttaaat atattaaaat tttttaatta gagtttaatt gcatccacta
     3181 tagccacatt ttaaacatat actacatcca cctgtatgtg caattccttc acttccacac
     3241 tcagggcaca caaatttttc atctccttca aaatttttat ttattatttc tttggctttt
     3301 tcatctttat tatctttttc ttttttatta tttttataat attctttttt actattttct
     3361 tttttatctt ctttattatt ttcttccttt ttttcattta ttaaaattcc actcctttta
     3421 catccatctc taaatattgt tactccttta agaccagcct cccatgcctt aatatatata
     3481 ttgaaaacat cttcaactgt agtttcaaaa ggtatattta ttgtagatga tatagaagca
     3541 tcaatatact tttgccatac tttttgcatg tttattcttt cttcatatcc tatattcata
     3601 gcagtaacaa aaaaatctgg taaacattct tcatctgtaa tacctttctt gtccatatac
     3661 tctttaacaa ttggagtata tactttataa tacacatcct catcatgcaa gctttcagtt
     3721 tttcttatat atgaaagatt aaatataggt tctattccac cactgatacc tatcattgta
     3781 gatatggaac ctgtaggagg tatggttaaa agttgtgaat ttctaagccc atacttttta
     3841 accatttcct tagtgtcttc atttagattt tctataaaaa acttagattt agaaatattt
     3901 tcatatttat actcactaaa tgttccatat tctttagcaa ttaatgctga ttgcttcaca
     3961 gcttcattta acatagtctt accaattaaa tcacatagtt ctaaagattc atcagaacca
     4021 tatcttattc ccatttttat aagcatatca gctataccca ttacacctaa acctatctgt
     4081 ctatacttat gtacactatc tctttgttct tgtaatggat gcaagttcaa tccctcatct
     4141 aagacatcat ttaaagctac tacagcttct ctaacgcaag attttaattt attaatatca
     4201 aaagtagcat gttcaccaaa tggttgtatt ataaactctg acaagtttat tgagccaagt
     4261 agacaacttc ctccacttgg aagtggttct tctgcacaag gatttactcc tgaaaagcaa
     4321 aagttcttat cttcactcat aaaattatat gtttttattt tatcccaaaa caatatacct
     4381 ggttctgcat aatcccagtt atttttcaca aatttattaa ataaatcata agcatttact
     4441 atcttaacta ttttttctcc agtagtttca acttcaaaag tacaattata taactttttt
     4501 tctcttacag cttgcataaa ttcatctgtt actcttacag aaatatttgc tttagtaacc
     4561 ttctccaaat ctgtttttat atctataaag tcttctatct ctggatgatt tacatccata
     4621 gaaatcataa gagctcctct tctccctctt tgaccaatta agccagttgt catactgtaa
     4681 agttccataa aagagactgc tccagtcgta tgttttgcag cattgttaac tttcgctcct
     4741 tttggtctta gctttgaaat atcaattcca actcctccac cataactaaa agttctagca
     4801 agtttttttg cagtatcaaa tatatcttct atgctatctt ttggtggttc tattacataa
     4861 caattagaat atgttacttt ttttccctct ttctgtaatc ctctatttgc taaaattctt
     4921 ccagcaaata gaaatttttt ttgtcttatt agttttgata ttcttgaact attatttgaa
     4981 attctcttta tccattcatc aaaactctca ccattatgtc tatacttgtt cttccatatt
     5041 gatttttgta attcatcaaa ctcccaagct ttagtcctta tttcagagcg tctttctcta
     5101 tataatatgt agtgttttgc tatatctttt ctatttgatt ccataagtag tacttcaact
     5161 atatcttgta tttcttctac ggataagcat ttttccatag aattatagtc attagctatt
     5221 tttcttgata taccatatgc caactctcta tctactccac cttcactatt tatactagct
     5281 ttatagacag catcttcaat cttactttta ctaaaaagtt cttttgtacc atctcttttt
     5341 attatgtatt tcatttttaa acctccaata aattagtatt aaattaatac acacattaac
     5401 tataactcta aacaaacttt ttatatattt gtatattttc atattaatta tctaacataa
     5461 tatatattat actattttat attcaatata acactcctta tagttttctt aacttgtaaa
     5521 atatcttcct aaaaaactca aaaataatat aaaaaagtat ataaaagtca aattatactc
     5581 tta
//
LOCUS       DAYAOY010000169         5533 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYAOY010000169 DAYAOY010000000
VERSION     DAYAOY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.173"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="ACGI6B_003824"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="ACGI6B_003824"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="ACGI6B_003825"
     CDS             complement(385..1569)
                     /locus_tag="ACGI6B_003825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9753231.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="ACGI6B_003826"
     CDS             1942..4293
                     /locus_tag="ACGI6B_003826"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HFG9753232.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="ACGI6B_003827"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="ACGI6B_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFG9753233.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          628 c          891 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAYAOY010000170         5492 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAYAOY010000170 DAYAOY010000000
VERSION     DAYAOY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.174"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            86..343
                     /locus_tag="ACGI6B_003828"
     CDS             86..343
                     /locus_tag="ACGI6B_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753234.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(453..1133)
                     /locus_tag="ACGI6B_003829"
     CDS             complement(453..1133)
                     /locus_tag="ACGI6B_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9753235.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            1393..1692
                     /locus_tag="ACGI6B_003830"
     CDS             1393..1692
                     /locus_tag="ACGI6B_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753236.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            2016..3671
                     /gene="ilvD"
                     /locus_tag="ACGI6B_003831"
     CDS             2016..3671
                     /gene="ilvD"
                     /locus_tag="ACGI6B_003831"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFG9753237.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            4015..4644
                     /locus_tag="ACGI6B_003832"
     CDS             4015..4644
                     /locus_tag="ACGI6B_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9753238.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            4670..5482
                     /locus_tag="ACGI6B_003833"
     CDS             4670..5482
                     /locus_tag="ACGI6B_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9753239.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2164 a          623 c          917 g         1788 t
ORIGIN      
        1 tttaaaaatt taaataatct ataaatcaat tataaaatat tatttattag aaattaaaat
       61 gaattaagta aatggaggaa ttgatatgat aataattaga agtcaagata aaactaattt
      121 aatgcagata aaccaaatta aaatagatgg tagtcaagtt tatgcagtat ttgaaagtaa
      181 aattgatact gtaaaaatag gagattatga aaataataca agagctatac aagtgttgga
      241 caatatacaa aacttcattg aaaatggaac aaaatatgat tatataacat caaataaagt
      301 cagatacaat gttaataaaa tatttcagat gccagctaaa tagtgagtgt gcattttaat
      361 ttaataacta aagcccactt tcttttgtag tatgaaagag ggctttacat atttgtaaat
      421 atcgaagtac acttatttta tcaataaatt atttaataag gtataatgga ccaattaaac
      481 aataaaaatt ctcctaaaac agcaagtatt aaatatataa tagcttcatt tttgtaaaga
      541 gaggtaattt ttttgcttat gtcatcatca attgtaatag caatctcatc ttgaataata
      601 tttttatata tagatgggaa aatatctttt ttctttataa gattaaataa attatttaca
      661 cctacccata taaaatatcc taaaacagca cctaatgtgt tcatcattaa atcatcaata
      721 tctgtagctc ttaagttaaa caattgacaa agttcaattg ctaatgaaaa acctaatcca
      781 gttaatgaaa cattgataat atttctaaaa tttttccaaa ttaaaggtag taaaaatcct
      841 aaaggcataa acataattat atttaaaata tatgttaaga tgccaactga gtcaaaaggt
      901 attagattta tttcatcaat tctaaaaagc ccactatacc ttcctatatc ccatatgcta
      961 ccaattccag ctttactaag tgctagatag atatataata aaaaaatata tacccatact
     1021 aaatgtatca ttacattctt ttcaactttc aaattttttc gttttataac gactatttgg
     1081 tatattaagc atggtaatat aacgcaaata aatgaataaa atgtccatat cattttattt
     1141 tgtccccttt caaatttatg tttttagttt tataagtact atctttatat taaactgtaa
     1201 gttttaaaaa tcttttaata aaatcttaaa aatttattaa tttataaaaa tatattatta
     1261 agattaaaaa atatatataa tcaaagtttc tacaattata tagttaggga tataaaaatt
     1321 ttatcgtatg cataaatatt ataaatatat ggaaataata atagtatatt ttggtaaagt
     1381 aggaggaatt ttatgaaatg gattaaatgg tatagtatta catgcatatg tatttttata
     1441 gtagtagctt tttatatgtt tatatttcct aataaaatag aaactattga tacttcatct
     1501 gcatatagct ttgttgaaaa gaaagtacca aatagtgctg tgtatcaagg atacaaaaag
     1561 aatccagttg atggtactac tacaatttat tacagctatg ataattcaac tcatatagtt
     1621 agattatcac atccagaaga ctattcaagg aagattaatt gggataaagt ttcaaacatc
     1681 agatttgatt aatctttaat atttataaaa gtctatatat ctaaggcaaa tatattatta
     1741 atcttattca tagttgagca ctttctatta tagaagtgcc tcttctgatt tatatcataa
     1801 aatattactc aacaatttgt aaaatatatt agtttataaa atatattgaa aagataatat
     1861 tatataatac gattgtgtaa gatattgata aaaaatcgta ataaattttc attagtatat
     1921 gctaatattt ttttacaatt ttcgtaatta attaatagaa aaatatatta taataatatt
     1981 atgaaaattt aaaaaagtaa gaaagaggta atattatgag aagtgatata aaaaaaggaa
     2041 tagaaggagc tccaaagaga gcgttaatgt atggaatggg tcttactaaa gaagaaattg
     2101 aaagaccatt aataggtata gtaaatgcac aaaatgaggt gattcctggt catctgcatc
     2161 ttgatgaaat agcagaagct gcaaaaaatg gagtaagaat gagtggtggt ttgccattag
     2221 aatttcctgc tataggggta tgtgatggta tagctatggg gcatgtcgga atgaattatt
     2281 ccctggcttc aagagaatta atagcagatt caatagaagc tatggctatg gcacatggtt
     2341 ttgatgcttt ggtacttata cctaattgtg acaagatagt gccaggtatg ttaatggctg
     2401 ctgcaagact taatatacca agtattgttg tgagtggagg gcctatgctt ccaggtaaaa
     2461 aaaatggaaa agtatatgat tttaactctg caatggaggg tgttggagca tgtaaagatg
     2521 gaacagtaag tgaagaagaa ttagaagagt tagccatgaa ctcttgtcct ggatgtggtt
     2581 catgttctgg tcttttcaca gcaaatagca tgaattgttt aacagaagct ttaggcatgg
     2641 gtataccata taatggtact gctgcgtcac attctggaga aagaaaaagg atagcaaaat
     2701 atgcaggtat gtatgttatg gagttactta agaacgacat aaaacctaga gatattttaa
     2761 caatagatgc ttttaaaaat gctatagctg tggatatggc aatggctggt tctacaaata
     2821 cagtacttca cttacctgca atagcttatg aatcaggaat agagcttaac ttagattttt
     2881 ttgatgaaat aagtgaaaaa actccttgtt taacaaaatt aagtccaagt ggaaaacatc
     2941 atattgaaga tttacatatg gcaggaggaa taccagctat aatgaacgaa ctttcaaaga
     3001 taaatggaat aaatttagat tgcaaaaccg taacaggcaa gactataggg gaaaatataa
     3061 gaaattgtga aatagaaaat gaagaagtaa tacatacatt aaagaatcca tatagtaacc
     3121 aaggtgggct tgcaatattg aaaggaaatc ttgctctaaa tggagctgtt gtaaaaaagt
     3181 cagcagttgc agaagaaatg ttagttcatg aaggaccagc aagagttttt aattcagaag
     3241 aagaagctgt aaatgctatt tttggtaaaa aaataaacaa aggtgatgtt atagttataa
     3301 gatatgaagg tccaaagggt ggtccaggaa tgaaagaaat gctatctcct acatcagcag
     3361 ttgcaggaat gggacttgat aagcatgtag cacttcttac tgatggtcgt ttttcagggg
     3421 caactagagg ggcatccata ggccatattt ctccagaagc tatggaaggt ggtttaatcg
     3481 gactagttga agaaggagat ataatttcta taaacatacc agacaaaaaa ttagagttaa
     3541 aagtagatga agttgaaata gaaaatagaa aattaaaatt taaaccttta gaaccgaaaa
     3601 taaagcatgg atacttaagt agatatgcta aactggtaac atcagcaaat acaggagcag
     3661 ttttaaaata gtagactata tttatgtaaa ttagaaaaag ttaagaaaat attaattgga
     3721 tatcttaggt gttatagcac taatgaggta tcctttttat tttgtatgaa aaacatagca
     3781 aaatcttgat tatctttgaa aacaatacat ctctattata aggaatacga gtacagcata
     3841 gagatgtatc ttttagacct tgttgaggta atacaacttt ttaaaagttt gatgtggaat
     3901 gcttttcgtt ccttctgaac tatgaagaaa ttgaaagtaa gttaattctt gacataataa
     3961 atattaaaat gtataataaa aaacaaacag acagtctgtt tttaggaggg aaatatgaat
     4021 agagaagaaa aaagtaaaaa tagtaaagaa aaaattatcc aatcagcatt ttcactattt
     4081 tcgtctaaag gatatgattc aacatctaca caagatatta tcaatttatc tggtctatct
     4141 agaggtgcaa tgtatcatca ctttaaaact aaagaagata tactgagaag tgtcacaaaa
     4201 gaactttact cacaaatgaa taatttttta gagcatcttg ttgctgatga cacccttaca
     4261 gcaaatgaaa aaataataga attggttgtt catagtgcga atgattacac acgtagaaaa
     4321 atggtacatt gtagctggtt agaaaaaatc ccattcgctt taatagagga agttcgtaat
     4381 ctcaacaatg tagttgcacc caatattgct aagataatta aacaaggtgt tgaaaataaa
     4441 gaattttctt gtgaatatcc agaggaatta gctgaaatgc tcgtttttag tattgatatt
     4501 ttacttgacc ctgtattatt taaacgtgaa tacagtgaag tatgtaatag gttggatttt
     4561 ttattattca tgttaaaaaa aatggatatt cctctaattg atgagtatgg tattcaaaaa
     4621 tttaaagatt tatttaaaca atgataatag gaaaagtgag gaatagacta tgttttataa
     4681 tgcaaaaagt tgtcaaatga tgataggaag taatacagtg aattatattg aatttggtag
     4741 tggtaagaag acactaatta ttttaccagg attaggagat ggtttatttc ctttacatgg
     4801 aaaaatacag gcgattgctt ttgcttttag atataagcaa tttacaaaag attataaggt
     4861 ttatgtattt agtagaaaaa atcaaattac agaaaaatat tccacaaggg atatggctaa
     4921 agaccaagca gatataatga aaaaacttgg aattatgaaa gcagaagtaa tgggtgtttc
     4981 tcaaggtgga atgatagctc aatatctggc aattgattat cctgagttag ttgagaaact
     5041 tgtgttggca gttacttctt caaagcaaaa tgatactatc caaaatgtta tttgtagttg
     5101 gattgatatg gcgaagaagc agaattacaa tgatttaatg attgacacag caaagaaatc
     5161 ctattctgaa agatatttaa aaaaatacca attatttatt ccttttttag gaaaagtagg
     5221 taaaccaaag gattttaaac gatttattat tcaagcgacc tcttgcatag aacataatgc
     5281 cttttccgaa ttaaataaaa taacatgtcc aaccttaatt ataggaggtg ctaatgataa
     5341 aattgttgga aataatgctt cttttcattt agtagaaaaa attaaaaaaa gcgaaatatt
     5401 tatctatgag ggattgggac acgctacata tgaagaagca caggatttta atgaaagagt
     5461 tttagagttt ttaaataagt aaacaccttt gt
//
LOCUS       DAYAOY010000171         5453 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYAOY010000171 DAYAOY010000000
VERSION     DAYAOY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.175"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            345..1058
                     /gene="rpsB"
                     /locus_tag="ACGI6B_003834"
     CDS             345..1058
                     /gene="rpsB"
                     /locus_tag="ACGI6B_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFG9753240.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            1137..2048
                     /gene="tsf"
                     /locus_tag="ACGI6B_003835"
     CDS             1137..2048
                     /gene="tsf"
                     /locus_tag="ACGI6B_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFG9753241.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            2133..2837
                     /gene="pyrH"
                     /locus_tag="ACGI6B_003836"
     CDS             2133..2837
                     /gene="pyrH"
                     /locus_tag="ACGI6B_003836"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFG9753242.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            2863..3420
                     /gene="frr"
                     /locus_tag="ACGI6B_003837"
     CDS             2863..3420
                     /gene="frr"
                     /locus_tag="ACGI6B_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFG9753243.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            3423..3626
                     /locus_tag="ACGI6B_003838"
     CDS             3423..3626
                     /locus_tag="ACGI6B_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753244.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            3655..4389
                     /locus_tag="ACGI6B_003839"
     CDS             3655..4389
                     /locus_tag="ACGI6B_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFG9753245.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            4402..5181
                     /locus_tag="ACGI6B_003840"
     CDS             4402..5181
                     /locus_tag="ACGI6B_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFG9753246.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         2216 a          579 c         1069 g         1589 t
ORIGIN      
        1 aaaaaaggtg atactatggg tattataaaa tattatacta attcaggtga cttacttgga
       61 agtgtagata tagtaagtaa taatagtata gataacgtat cattaaaaac aaaattaaaa
      121 atgaaatttg caaattaaat tcaaaaaatg gtataaaata cacaaaaaaa tataaaaata
      181 gaaaatatgg aaaaaatatt gttatttctt cacctctgtg atatactact agaggtgaaa
      241 aaataaaata aatcacacac agcattggga tccttaaaag gtgcctatta catggttttt
      301 tagggagaag atcaagctgg aggtataaaa ccaggaggta agatatgtca gtaatatcaa
      361 tgaaacaatt attagaagca ggtgttcact ttggtcacca aacaagaaga tggaacccta
      421 aaatggctaa gtatatattc acagagagaa atggaatata tataatcgac ttacaaaaaa
      481 cagttaaaaa agttgaagaa gcttacaagt tcactaaaga agtagctgaa acaggaaagc
      541 caatcttatt cgtaggaact aaaaaacaag ctcaagatgc gataaaagat gaagctgaaa
      601 gatgtggaat gtactttgtt aatgaaagat ggttaggtgg aatgttaaca aaccacaaaa
      661 ctataaaaac aagaataaat aaattaagag aattagaaaa aatggaagaa gaaggcgtat
      721 ttaatgttct tcctaaaaaa gaagttataa aattaagagc tgaaaaagaa aaactagaaa
      781 aatacttagg tggaataaaa gacatgcctg aattaccagg tgcaatgttc gtagttgacc
      841 caagaaaaga aaacatagct attcaagaag ctcatagatt aggtatacca gtagttggta
      901 tagttgatac taactgtgac ccagaacaat tagactttgc tataccagga aatgatgacg
      961 ctataagagc cgtaaaatta ataactggtg ctatggcaac agctgtaatt gaaggtagac
     1021 aaggtgctga ggaagaagta gctgaagacc aagaataata atttgacaat taaatggtaa
     1081 gggtcgccct taccatttat aatatggaat agaataagta ggaggaaact taaaaaatgg
     1141 ctaatataac tgctcaaatg gtaaaagagt taagagaaag tacaggcgca ggaatgatgg
     1201 actgcaagaa agctttacaa gaagctgaag gaaacatgga aaaagcagta gatttattaa
     1261 gagaaaaagg attatcaaaa gctgctaaaa aagctggtag agttgctgct gaaggtctag
     1321 ttgcaataga aatgaatgat gataatacag ttgcttctat ggtagaagtt aactcagaga
     1381 cagatttcgt tgctaaaaac gaagacttca aagtgttcgt taaagatgct gcttgtatgg
     1441 cattagctac tgataaagaa gatatagctt ctttattagg tgaaactcat aaagagggaa
     1501 taactttaca agaagtttta aataatagag ttgcaaaaat aggtgagaaa ttagacttta
     1561 gaagatttgc taaagttgta actaatggac aagttgctgg atacattcac ggtggtggaa
     1621 aaataggtgt tcttgttgaa atggaaacag aagctagaga tgctaaagtt ttagaattag
     1681 gtaaagatgt agctatgcaa gttgctgcta tgaatcctaa gtatgtttca agagatgaag
     1741 tagatgctga atacatagca catgaaacag aagtattaac tcaacaagct ttaaatgaag
     1801 gaaaaccagc taatatagtt gaaaaaatgg ttaaaggaag attagaaaaa gaattaaaag
     1861 aggtttgttt attagagcaa actttcgtta aaaatccaga tataactgtt aaacaattag
     1921 ttgctgatgt tgctaaggca gtaggttctg atataaaagt tgttaaagtt gtgagattcg
     1981 aagttggtga aggtatacaa aagagagaag aaaactttgc agaagaagtt gctaagcaac
     2041 ttaagtaata accaaagaga gaacacgcag gtgttctctt ttttaaaaaa tagaaaaaat
     2101 tggatatgaa ggattgaaat aggaggtttc tgatggataa accaatgtac aaaagggtat
     2161 tgttaaaatt aagtggagaa gcattagcag gagaaagagg ttttggtata aataatgatg
     2221 tagttaatga tattgctatt gctataaaaa aaatacaaga aataggtgtt gaagtagctg
     2281 tagtagtagg tggaggaaac ttctggagag gcagaaccag tgaaggaatg gacagaacta
     2341 ctgctgacta tataggaatg ttagctacag ttatgaatgc aatggcgcta caggatgcac
     2401 tagaaaatat agatgttgct acaagagttc aaactgctat agacatgaga caaattgcag
     2461 aaccatatat aagaagaaga gcagttagac acttagaaaa ggaaagagtt gttatatttg
     2521 gtgcaggaac aggaaaccct tactttacaa cagatacaac tgctgcttta cgtgctgctg
     2581 agatggaagc agaagttata ttgttagcta aaaatgttga tgcagtatat gacaaggatc
     2641 caaaagtaca tgcagatgct aagaaattta cagaattaag ttacatggaa gttatacaga
     2701 aagaattaaa agtaatggat tctacagcta catctttatg tatggataat aaaattccaa
     2761 taaaggtatt tgagcttaca acagagaata taataagagc agtaaaaggc gaaaatatag
     2821 gaactactgt aaaataatga agaatgaagg aggagtaaaa ttatgaaact ggaaatacat
     2881 aagcaattag aagagaaaat gaatgggaca attgatgctt taaagtttga atttggaact
     2941 ataagagcag gaagagctaa tgcacaaatg ctagacaaaa taagagttga ttattatgga
     3001 actccaactc caataaatca aataggtgct atatctgtac cagaaccaag aatattaatg
     3061 atatctcctt gggataaatc tgctatgcat gaaatagaaa aagcgatagc taactcagac
     3121 ttaggattaa atccatcaaa tgatggtgag gttataagac tttctgtacc tgctttaaca
     3181 gaagaaagaa gaaaagaatt agctaaaaaa gctagtaaag ctgcagaaga atttaaagtt
     3241 agaataagaa atgaaagaag agacgccaat gagaaaatta aaaagatgga aaaaggcgga
     3301 gaattaactg aagatgaatt aaaaaaagca caagatgaag ttcaaaaaat gactgataaa
     3361 tttataaaag aaatagatgc attattatct aaaaaagaaa aggacataat ggaggtataa
     3421 tattgaaaaa atattataac cttttaggtc ttcatgtaga tgaggtaaaa caatattttg
     3481 atgagcaaaa tataaattat accgtaaaat ctattgaagg taaaaaggat aaagaaaaac
     3541 ttgtagttcc tagagttata aaaatatctg agataggtga tagtgtagaa ctgataatca
     3601 catacttttc tgactcctta gtttaaggag tcagaacaat attggaggca aaatatgaat
     3661 aacaacatta tatatgatat agacttaaat aacataccta ctcatattgc tattataatg
     3721 gatggaaatg gaagatgggc aaaagctaga tttcttccaa gaactgcagg acataaggct
     3781 ggagtagaaa ctataagaga tatagtaaaa gaatgctcaa aattaggtgt taagcactta
     3841 acattatatg cattttcaac agaaaactgg aaaagaccga aacttgaagt tgacacactt
     3901 atgaacttat tatctacata cttaagaaat gaaatagctg aacttcacca aaacaatgta
     3961 aaagtaactg ctataggaga tataagtgct ttaccaaaaa cttgtataag agaattaaac
     4021 tcagctaagg aactgacaaa aaacaataca ggagttaact taaatttagc attaaactac
     4081 ggaagtagag cagatataaa aaatgctctt atagatatag taaaaaattg tgaaagtggt
     4141 aaaatagata taaacaatat tgatgaagat attataaaaa actatttaag cacaaagtct
     4201 ataccagatc cagacttggt aataagaaca agtggtgaac aaagattgag taacttttta
     4261 ctttgggaag ttgcttattc tgagttttac tttactgata tacattggcc tgattttaaa
     4321 aaagaagaac tacagaaggc tatatacgta tatcaaagaa gggatagaag atttggagga
     4381 cttaagtaag gagattattt tatgcttaca agaattattg cttcactagc tctggttcca
     4441 ttatttctat ttgttgtata tggaggtatt cctctatata tagctgagac agcaatagtt
     4501 tatattgcat tgcatgagtt ttataaggca tttaaaataa aagatgtaca tccaatattc
     4561 attataggat atttattttc tatttattta gctgtaaaaa atatttttaa tttaccatta
     4621 gaatatactt atgcagtaat ttttatttta tttttagcaa gtataattta tatgttgatg
     4681 ggcaaaaaca atgttataga tgtttcgatt acgtttttag gagtttttta tataggagta
     4741 tttttagatt ttataattat aactataaat ggatttgaaa aaggtagtat atatgtatgg
     4801 cttatatttg tgatatcttt tatgacagat atatttgcat attttagtgg ttatttactt
     4861 ggaaaacata agctaattcc taaagttagt cctaaaaaga ctatagaagg agctattgga
     4921 ggaataatag gaagtacact ttgttgtatt ttatttggat atttatttgg cattgatttg
     4981 cttcaattag cgataatagg aagtatagga agtgtaatag ctcaattagg agatttattt
     5041 gcatcttcta taaagagata tgtagggata aaggattatg gaaagataat acctggtcat
     5101 gggggtatat tagatagatt tgatagtgtt atattagttg caccttttgt atatagtgcg
     5161 attaaatttt ttattagata aagattggtt ggatttaaaa gagcaatcta taattgctag
     5221 taatgaaaat agctatgtaa cttttaaatg cagagatatt ctgcagttat tagttacatg
     5281 gctattttca ttgttcaaaa atttagaaga ccttggggaa ttgtataaga ataggtatta
     5341 ctttataata gtatactata tttgtagtga gtatattttt tatactgtaa ctatcattaa
     5401 aaaatatttt tttgtttaat aatttcgata tttttagctt tattatatat tta
//
LOCUS       DAYAOY010000172         5321 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYAOY010000172 DAYAOY010000000
VERSION     DAYAOY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.176"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(642..2777)
                     /gene="rnr"
                     /locus_tag="ACGI6B_003841"
     CDS             complement(642..2777)
                     /gene="rnr"
                     /locus_tag="ACGI6B_003841"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFG9753247.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3370..3984
                     /locus_tag="ACGI6B_003842"
     CDS             3370..3984
                     /locus_tag="ACGI6B_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFG9753248.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            4002..4364
                     /locus_tag="ACGI6B_003843"
     CDS             4002..4364
                     /locus_tag="ACGI6B_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9753249.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4470..4967
                     /locus_tag="ACGI6B_003844"
     CDS             4470..4967
                     /locus_tag="ACGI6B_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFG9753250.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1761 a          783 c          628 g         2149 t
ORIGIN      
        1 ataaattcta aaaaagaaaa gttatcttaa ataaaaaata agtgtatctt aaattttaaa
       61 tgatacactt atttttatat tgaaattata ttttttaata tctattctta attaggtatt
      121 tttaaattat ctgtttacat taaatatttc ttaattactt atctttcagc tagctttttc
      181 tattaaatgt ttctcaatta cttatttttc aattagccac ttatattaag catttcttaa
      241 ttacttattt ttcaattagc cacttatatt aaacatttct taattattta tttttcagtt
      301 ggctttttct attaaatgtt tcttaattac ttatctttca gttagctctt tctattaaac
      361 atttcttaat tatttatttt tcagttggct ttttctatta aatgtttctt aattacttat
      421 ctttcagtta gctctttcta ttaaacattt ctcaattact tatttttcaa ttagctactt
      481 tcattaaata tttcttaatt atttatcttt cagttaacta tttccattag atatttctca
      541 attacctatt tttatcattt attttataga gtattgtgca ctatatattt atacatatca
      601 taaatactac aacttgttat ctcttatttt agctgtttta tctactcctc tattttttca
      661 actatctcaa agtcaatttc tttaaaatct acattaacac tttcaacctt tatcttaaca
      721 aggtctccta tcttatatga cttcttagtt ctttctccta aaacactata agaactctca
      781 tcataaatat agtaatcatc attcatattt gcaagtctta tcaatccttc tatggtatta
      841 tccaactcaa caaatattcc aaatgaagtt acgctagata caacaccctc aaattcttga
      901 ccaactttat ccttcatata aagagcctta tagaaatcat gtacatctct ttcagctaac
      961 tctgcctttc tttctttctc ggatgattga acagatgcat aatccactat agtagtaagc
     1021 tgttcttgtc tttttccact tattttatta tttaaagatt cttttattat tctatgaatt
     1081 tgaaggtcag gatatcttct tattggtgaa gtaaagtgac agtagaattt agctgctaat
     1141 ccaaaatgtc cactacattc tggtgagtac ttagcttgtt ttaaagaacg aagcattata
     1201 gtactgatag ctccttcttc ttttttgccc tttattttct ctattatacc ttgtaatgct
     1261 tttggatgta cttcctcttt atcacctttt attatatatc caaatgtaga tataaactta
     1321 ctcaattctt ccattttttc caaagatggt gtctcatgta ttctatacac aaacggtatt
     1381 cccatccagt aaaaatgttc ggcaattgtc tcattactta taagcataaa ttcttctatt
     1441 atcttattag atattcttct ttcatatggc tttatatcta caacttcgcc ttttccattt
     1501 agaattatct ttgcctctgg gaaattgaag tctatagctc ctcttttttc ccttcttgac
     1561 ataagaatcc tagctaaaat ttcagcattt ttaaaatctt caacaacatg ttcaaaagtc
     1621 tttttaagtt tttcatcatc cttttctaat atatcagata cttctgtata agtcattcta
     1681 gcttttgaat ttattacaga ttcacatatt tcatgtttaa ccacatttcc tttatggtct
     1741 atttccataa atatagaaag agttaattta tcttcaaatg gatttaagct acatactcca
     1801 tttgaaagag tttttggcaa cattggaatt actttatcta ctaaataaac tgatgtagct
     1861 ctttttaagg cttccttgtc aagtttattt ttctctctta cataatgtgt aacatcagct
     1921 atgtgaactc ctagtttgaa gtttccatta ggtaatactt ctattgatat ggcatcatct
     1981 aagtcttttg cgtcatctcc atctatagta aatatgttta aatctcttaa atctcttctt
     2041 ctttttatct cttcttgagg aatctcaaca gctacagctt ctgcttcttg caatactttc
     2101 tttggaaatt cttctggaag accatgttct cttattatag aatctatttc tacgcctctt
     2161 tcaccttttt gacctagtat ctctataatt ttgccttctg gttttctatc ctcttgtggc
     2221 catactgtaa tttcacatac taccttatca ttatctactg ctccactaaa atgtttttta
     2281 ggtatatata tatctttagt aaatttcttg ttgtctggtg ttacaaatcc aaaagtttta
     2341 cttgattgga aagtacctac tattctagtt atttctctct ttattacttt tactattctt
     2401 ccttctgctc tcttttcttc agttgcaggt actactattt ctgccataac tctatcatta
     2461 tgcatagcac catttatatt gtcacttgag ataaataaat cctgtgtata ttcaacatca
     2521 gattctacaa atccaaaacc ctttttgtgt gatacaaatt ttccaacaaa gtatcccatt
     2581 tgattgggag atactatttt tcctttttta gtaaagcaga tataaccatc ttcttctaat
     2641 tcatttaaaa aattataaaa cattggcatt tctgatgaat gtatatcaaa tatttctgct
     2701 aattcttctt tttttagagg attatatgca ctttcattta ttaatcctag caaactttct
     2761 ttaagtcctg gtatcatttt tccctcctgt tttacttttc attatattat tatttatatt
     2821 gtcttttata tttcttcaga caaaacattt tttaattatc acataatttt ctatattttc
     2881 ttattattat aagttaaaat tacttattta tatttttgat ttaatgtaag ctttgtcata
     2941 ctaacttcaa acaaattatg atgttttata aattatttgt aaatatcatt ataattactt
     3001 aagttattct ttatcataat tgcttaaatt actctttatc acctaattat acaccatcaa
     3061 ataggattgg taaaatattc atctcatata agtattacta tataataatt tattctatta
     3121 gtatactcaa atcatgtaaa atttatataa ctcaattaat tttttaaata caactttata
     3181 atataaaact tataataaaa gtaaatttat atgttaaatg aggttgattt tttattattt
     3241 tttatttatg ctagtactaa agacttgcaa acgttttttg acaagtcttt attttgtcta
     3301 aattaaacaa acttttttac aaataaatac ataaaattta aactaatcta tttattggag
     3361 gagataaaat tggaagaagt aattgcaaaa attattacta tagaaaatgg attcaaagaa
     3421 attgaaaaca ttgctaaaaa aattgtaaaa aataatgact caaaaaagtg ttattatcta
     3481 tcaatctact tatataggtc agaatattat caagtaagag aattggctgt gtttatactt
     3541 ggttttatat cagtaactat ttctgaagct ttgttatttt taaaagataa tgtgagtaaa
     3601 gatgaaaact ggagagtaca agaaatacta gctaaatcat ttgattatta ttgcagtgaa
     3661 gtaggatatg aaaaggctct tcctgttata aaagaatggc ttaattctga tagtccaaac
     3721 ataaaaaggg ctgtgacaga aggtcttcga atttggacta gtagagacta ttttaaatct
     3781 aatcctgacg ttgctatatc actactttct agtttaaaag aagatgatag tgaatacttg
     3841 agaaagtctg taggtaatgc tcttagagat ataagtaaaa aatatccaga tttagtaaaa
     3901 aaagaattag attcatggga tatatcaaat aaaaaagttg agtttgtata taagcttgct
     3961 tcaaaatgta tcttggcaaa gtaaaatgga ggtgaactta tttgaaactt cgtgatgagt
     4021 atacttgccc attagaacta acacatgata tgataaaagg caaatggaaa tctattatat
     4081 tatggagatt acgcatagga ccaacttcct tatcaaaact tgaaaaggaa atcaatggta
     4141 ttaatcaaaa gatgttactt gaacaactta aagaattaat tgaatttggt cttgtagata
     4201 aaaagacata tgaaggttac ccacttaaag ttgaatactc acttacacct attcgaggga
     4261 aacaaatatt ggatgctttg gctataatgc aagatatagg aattagcttt atgttggata
     4321 atggtatgga agatattctt aaggaaaaag gtgttttaaa ataaatagat acccacaaaa
     4381 aagtaagtaa tttaccatta ttttcaactg gtatataatt aaattataga tatattctat
     4441 taatttatta taataggagg taaattatta tgaaagtaac tagtacaggt atagtaaatg
     4501 gtgttataga agataaatac ggtaaaagag gaactcagtt caatgaaggt ggtatgccta
     4561 catattcact acctctaaag tttgaagatg ctccaaagaa tacagtatcc tttgctgtat
     4621 ttttagaaga taaagactct gttcctgtat gtgggtttgc atggatacat tggttagctg
     4681 taaatataac aaaagatgag ttaaatgaaa atgaaagtgt aacagctact gattttgtac
     4741 aagggactac tagttggcat ggcaaactag gaggtcttga cagattagag gccagtgtat
     4801 atggtggaat ggctcctcca gacagacctc atgtctatga aatacatgta tttgcacttg
     4861 atactatcct tgaccttgaa aaaggattct atatgaatga atttttcaaa gctattgaag
     4921 ggcatgtttt agatacattt acactaaaag gaagttattc taattaatta ggttgactat
     4981 agctaatcat taattaaatg ctacatttaa ttgatgatta gcttaattgt tattagaata
     5041 ttttctgcac tttatcttaa tatatatttt agttgactgg tattttaatt tctattagat
     5101 tattatcaat ttatataaat tgattttagc ttaaaactta cttatctatt tcagtttcta
     5161 ttagaattat tattagactc attaaaataa aagcactaat ataactaata atatatgtta
     5221 aattcattaa ctctacataa agttttataa tctaaatgaa atacaataat ctgcataaaa
     5281 ttcaatagct tatataaaat ttaataattt atataaacat t
//
LOCUS       DAYAOY010000173         5310 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYAOY010000173 DAYAOY010000000
VERSION     DAYAOY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.177"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            204..374
                     /locus_tag="ACGI6B_003845"
     CDS             204..374
                     /locus_tag="ACGI6B_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9753251.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(550..1089)
                     /locus_tag="ACGI6B_003846"
     CDS             complement(550..1089)
                     /locus_tag="ACGI6B_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFG9753252.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(1228..1860)
                     /locus_tag="ACGI6B_003847"
     CDS             complement(1228..1860)
                     /locus_tag="ACGI6B_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFG9753253.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1996..3909
                     /locus_tag="ACGI6B_003848"
     CDS             1996..3909
                     /locus_tag="ACGI6B_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9753254.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3925..5301
                     /locus_tag="ACGI6B_003849"
     CDS             3925..5301
                     /locus_tag="ACGI6B_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9753255.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
BASE COUNT         1886 a          735 c          781 g         1908 t
ORIGIN      
        1 taacttttta aatttaattt tatttttaaa ataaacttct ttaaaataaa taaaaatata
       61 agcagattaa actttattgt aaaactttaa aacaagaata tcataataaa gtttttgttg
      121 tagaattaat ttaattgtta tattcaataa tatttgttat aatgttattg aattacaata
      181 catataaaag ggaggatttc aaaatggcat ataaaattac tgacgcttgt ataagctgtg
      241 gtgcttgtga ggctgaatgc cctgttagct gtatatcagc tggagacgac gcatatgtta
      301 tagatgctgg ttcttgtata gactgtggtt cttgtgcagg agcttgtcct gtagatgcac
      361 ctcaaccaga ataataattc gttattcata aagagactga ttttatttta taaatactaa
      421 tcagcctctt tttaattatg tatgattcaa ttaatattat ttatatactt tattatgttg
      481 ctacctaatt catcttaaaa tactgttagc acatatctaa tacttttaac tttaatctaa
      541 tttataattt taatactcct cataaaatct ttcatattta ttaaagcaat caagacatac
      601 tttctttcct tcttcaatcc ttatatattg ctcagatgca aattcaccac acacttcaca
      661 tttaagagat ttaaatattc ttgctttttc aggaagtctt aacttagtat ctgtaatctc
      721 aaatatttct tcaaaatcac tttctaataa gtactttttg tatccttctc tatccatttg
      781 aaatggcttt gactttacat atattcttat agattttcca ttttccctat taaagaaatg
      841 atatactgat ttcccagtca tcctaagtag taaatttcct ttacccattg tgcaacttag
      901 cacgacttgt ataccatcta ttccacaagc atcattttca gaaatacata caatttcctc
      961 atctcctgac tgtttacatc ctaaatattc tcttgctgca agtgctgctt taaatcctat
     1021 agctaaccct ggacattcat gaccatgaaa ttcaattgcc tttttccata attcctccac
     1081 aaaaaccact ctccttaata ttgtttattt ccatacatta tttaaatttt tatcctaata
     1141 tttcaaaaag ccagaggatt taatattaaa tcttctgact ttctcaatac tcttcacaac
     1201 tgctcattat taatatgttt aagttgtcta ctttccttct ttcattaaat acgataaggt
     1261 aaataaactt tctgttaagt ttacattttc aactataaca cctttcggaa cttctaaatc
     1321 taaaggtgca aaattccata cacctttgat tccagctttt acaactctat taataacttc
     1381 ttgtgctcca tttttaggta tacataatac agcaatatct atattattgt tttttataaa
     1441 gtcttctaaa gtatctgaat ctaatacttc aaactctctt atctttaaac ctatcattct
     1501 aggatttgca tcaaataaag cttttatctc gaatccagct tttctaaatc ctgcataatt
     1561 ggctatagct tgtcctaagt tacctgctcc tacaagaact gcgttgtatg gtcgatccaa
     1621 cccaagaatt ttacctatct cagtatgaag agcttctaca ttataaccat atccttgttg
     1681 tccaaatcca ccaaagttgt ttaaatcttg tcttatttga gaagcggtaa accctattat
     1741 atcactcaat tctttagaag atattctttg tatatcccta tctaataagt ctccaagata
     1801 tctatgatat tttgggagcc ttcttataac tgccattgat atatttttat ttcccaacat
     1861 cttcacctcc taaaagtgtc ctcttttttg tttgattcac aataaaatat ttctttttca
     1921 aaactaatac atataacctt gattatatta tatcaaaaca ttataatttt agatagttca
     1981 aggaggtaga aatttatgat tgttttgtca tgtaataact taaataaaag ttttggtata
     2041 gattctatat tagaaaatat tagttttaca gtaaatgaag gcgataaaat tggtataatc
     2101 ggtgttaatg gtactggaaa aactacttta tttaaaataa tttcaggaat ttatggatat
     2161 gatagtggag atatttacac ttctaaagat tgtgaaatcg gatatttaga acaaaatacc
     2221 aatttttatt cggataatac tattttggaa gaagtactag aagtttttaa aaatttgata
     2281 gaaatggaat cctatttaag agaattggag gttaaaatat ctgaagaaag tacaaaaaca
     2341 aactctccaa ttatagaaaa aatcatggat gaatattcac ataaactaga attattttca
     2401 gatttaaatg gatatggata taaatctgaa gccaaaggag ttctaaaggg attaggattt
     2461 agtgacaatg atatggataa gccaataagc atactttctg gtggtgaaaa aactagagtt
     2521 cttttaggaa aattacttct taagaaacca actctattgt tacttgatga acctactaac
     2581 cacttagact ctgaagcaat agaatggtta gaagtatttt taaaacaata taaagggact
     2641 gtcatgttaa tatctcatga tagatatttc ttagaccaat ccgtaaatag aatttttgaa
     2701 gttcacaaca aaaaacttaa agtatataat ggtaactact ctaaatttgt tgagttatct
     2761 aaaatagaaa aagaactaga gcttaagaaa tttgaagacc aacaaaaaga aataaaaaag
     2821 caagaagaat caattgaaag actaaaagct tatggtagag aaaaacacct aaagcgtgca
     2881 agaagtaaag aaaaagcatt agacaaggta gacgttttag ataaaccaga agcttatagg
     2941 aaaaaagctc gtatacaatt tacgccttct gtccagagtg gtaatgatgt tcttcaaatt
     3001 agagatgtat ctatgggtta tggagaaaga atacttttta aagacttgga tttagatata
     3061 tatagagggg aaaaagttgc acttataggt gcaaatggtg ttggcaaatc tacattattt
     3121 aaaataataa ctaatgaact acaacctctt agtggcaata ttaaatttgg aactaatgta
     3181 catgtttcct acttccatca ggaacaaaaa actcttaatt tagataacac aataattgat
     3241 gagatttggg aaaataatac tcatcttact caaactactt tgagaaatat gttaggggca
     3301 tttttatttg tagatgaaga agtttttaag aagatttcta cattgagtgg tggagaaaga
     3361 gctagagttg ccatacttaa acttatacta tcaaatgcaa acttattgtt attagatgag
     3421 cctactaatc acctagatat tgattctaag gaagtccttg aagaggcatt aactaattat
     3481 gatgggacta tatttacaat atcacatgat agatactttc taaatactgt tgttgataaa
     3541 attttagttt tagatgaaaa tggaattact gaatatctgg gaaattatga ttattatata
     3601 gataaaaaaa ggcaaatcca agagatgagt attatagaag aaaaagaaga aaaaactaga
     3661 actcaaataa aagatgaaaa aagaaaagag agagaacaaa gagaaataga aaagaaaaat
     3721 agaataaaaa gacaaaatat agaaaaagaa attgaaaagt tagagattga aattgaaaaa
     3781 ttagatatac ttttatgtca agaagaagta tactctaatc cagataaagc taaagaagtt
     3841 agccaagaaa aaattaattt agaaaataac cttgcctctc tatatgatga gtgggaagaa
     3901 tttatgtaaa aaggagagga ttatttgcaa gaactatttt ctttaaaaga cgaaaataaa
     3961 agattttata aaatacttct atctttatgt atacctatta tcatacagaa cttaatttct
     4021 acttctgtaa atgtaataga tacaattatg ataagtagtt tgggggaaac atctgtagct
     4081 tcagttggag ttgcaaacca atttttcttt ctttttaata tgtccttatc tggtatcaca
     4141 ggaggtgctg gagtttttat ttcacagttt tatggaaaga aggatgtgag taatataaga
     4201 aaagtcacag gtcttacttg tgtattggca attgttttaa gttttgtatt tgttattcct
     4261 gctttactaa caccaaagcc aattatacac atattttcgt atgattctga ggtggtaaaa
     4321 ctttgtatag attactttag tattgcagta tttagttatc cattaattgc tgtaagtact
     4381 gtatttagca ctggttcaag aggtgttaga aatcctaagt taggtatgat ttgtagtgca
     4441 tttgcacttg taacaaatgt tattttaaat tatggattta tatttggtaa cttcggtctt
     4501 cctgcattgg gtgtaaaagg tgctgctcta gctactgtta ttgctagaat atgtgaatta
     4561 attttaatga ttacttatgt ttacctttat aaaaaagact atatattaaa gtttggtcta
     4621 aaaaatctaa aagctattga taaaattttt ataaaatctt tttcatcaaa aagctttcct
     4681 atatttgtaa atgactctgt atgggcaatt ggaactgtcc tgtattctgt tgcatatgca
     4741 agagctggta cttctgctat agcagctagc cagatagcaa ccagtacagg taatttcttc
     4801 ataatgacag ctgtatgtat tgcatctggt gcatctatta tgcttggaaa tgaacttgga
     4861 gctgaccata ttaaaagagc cattgaatat gctaaaaagt tttctatact tgtattttca
     4921 gctggtttaa tacttggaat tattttaatt ttaaatatac cattattatt aaaaatgttt
     4981 agtgtttctg atagcttagc ttctgatata actaagatat tctttataat gggaatacta
     5041 atggctctta aatcttttaa tacattggtt ataattggta ttttaagaag tggaggagat
     5101 actaaatatg ctctattttt agaattagga tgtatgtggt tagcttctat tcctctaact
     5161 tttatagctg catttaaagg tgcacctata tttgtactcg ttttgctcac ttatagtgaa
     5221 gaagtggtta agtttatatt tggtgttcct agagcattat ctaaaaaatg ggctattaat
     5281 atagtaaagg aaattgatta ataattatac
//
LOCUS       DAYAOY010000174         5248 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYAOY010000174 DAYAOY010000000
VERSION     DAYAOY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.178"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..783)
                     /locus_tag="ACGI6B_003850"
     CDS             complement(<1..783)
                     /locus_tag="ACGI6B_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFG9753256.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNID"
     gene            complement(941..1594)
                     /gene="phoU"
                     /locus_tag="ACGI6B_003851"
     CDS             complement(941..1594)
                     /gene="phoU"
                     /locus_tag="ACGI6B_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFG9753257.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(1622..2386)
                     /gene="pstB"
                     /locus_tag="ACGI6B_003852"
     CDS             complement(1622..2386)
                     /gene="pstB"
                     /locus_tag="ACGI6B_003852"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFG9753258.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(2405..3229)
                     /gene="pstA"
                     /locus_tag="ACGI6B_003853"
     CDS             complement(2405..3229)
                     /gene="pstA"
                     /locus_tag="ACGI6B_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFG9753259.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(3231..4148)
                     /gene="pstC"
                     /locus_tag="ACGI6B_003854"
     CDS             complement(3231..4148)
                     /gene="pstC"
                     /locus_tag="ACGI6B_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFG9753260.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(4529..5002)
                     /locus_tag="ACGI6B_003855"
     CDS             complement(4529..5002)
                     /locus_tag="ACGI6B_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753261.1"
                     /translation="MFVICISIYLAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQ
                     ILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGG
                     IGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
BASE COUNT         1726 a          954 c          591 g         1977 t
ORIGIN      
        1 atctatattc cagtctagct tatccacatt atatgttttc ttaaggtatt tatatgtatg
       61 ctctttcaaa taaagtttat tttcaagtaa ctctatagac tgcatatgaa aatgtatttc
      121 tctacatatc cttaaggcat tctcaatttt ttctatatta gtttcactca aactataatc
      181 caactcatca ataagttttg aatatatttc ttctaatttt tctacttctt cttcaaattt
      241 ctctacattt aaattttctt ttctaagaat tttatcttca ataagcaaca ttgaaagttc
      301 ttctatcttt ttaaactcat ctataacact atttagataa tttggtctag caacataata
      361 attaacaagt actccaaaca aaacacccac agatgtatct aacaccctat gtaaagaata
      421 atacacaggg tcactatttc ctacacctag atgtatagcc agaaaggtta cacaggcaac
      481 tactactgac tttattttaa acagattgca taaatatatt gagcacatta ctcctagacc
      541 acataaaaat gggtctccag ctttaatgat ggcaaatatg aatcctatta taccacctaa
      601 aatagtacct tccactctat tgattcccgt ctttaaagaa ccttttactg tatcttgaac
      661 agaaattata catgctacca gagaataaaa catattctct actaaatagt caccaagaat
      721 catacataaa gtgacactaa ttcctgtttt tatagtcctc atacccatct tttgaagttt
      781 caaagttcca cctcctaaaa agcaccttat ataaaatacg catcctcata tttaatatgt
      841 tcctagtata tcacaatttt ttaaatttac ccattaatca tattttttta tcaattcttt
      901 cttaatttag acaataaaaa aactgcttta aagcagtttt ttatcctatc tcaaccattt
      961 taccctcaat tgcaaaaatt atcttctcac atacatttgt tatatggtct ccaattcttt
     1021 caaggtatct tcctataagt agaagtttta ccccttggtc tatggtattt gagttttcat
     1081 gcattatttt taaacaatca atttgtactt gttcatacaa tccatctaca atatcatctt
     1141 tagttccaac atcataagct atacttgcat caaataagat taaagcttct ctagtctgag
     1201 acatcataga cttacattct tttgccatct taggaatatc tattagctct tttatataag
     1261 cctctccacc tatcttcaac atttctctag ctatatttgc tgcataatct cctattcttt
     1321 ccagctctat agatatatta cctaatgaat aaataaatct taagtccctt gccattggtt
     1381 gctttaatac tattagctct atactcttat ctcttatttc ttctcttaat ttatttattt
     1441 cttcatcatc ttctactact tttcttgcac cctctaaatc cttctctatc atacaagtca
     1501 cagacttttc tattgcttct tcacattttt ccatcataag cactgtttgt tcttttaaag
     1561 tatcaatact tatatctaag ctattattga ccattgtcta actcctttat agttatatat
     1621 tttaaccaaa tctacctgtt atatagtctt ctgttctttt atctacagga gtggtaaaca
     1681 tagtttttgt atcgctaaat tctataacct ctccatttaa gaaaaaagct gtttcatctg
     1741 atatacgagc agcttgttgc atattatgtg taactatgac tattgtgtag tcttttttta
     1801 aatcctctat aagttcctct acttttaatg ttgaaattgg gtctaaagca gatgtaggtt
     1861 catccataag aataacttct ggtctcatag caattgctct tgctatacat atacgttgtt
     1921 gttgaccacc agaaagtcct agtgcagatg atttaagcct gtctttaacc tcatcccaaa
     1981 tagctgcacc ttttaagctt tcttctacaa ttttatctaa ttgcttctta tctcttagtc
     2041 catgtgtacg aggaccatat gccacattat catatatact cataggaaat ggatttggct
     2101 tttgaaatac cattcctact ttagtacgaa gatttataac atcatctgag gtatatatgt
     2161 cctctccatc aacagaaatg tttcctttaa ttgttacatc ttcgattaag tcattcattc
     2221 tatttaacgt tcttataaaa gttgattttc cgcatccaga tggacctatt aaagcagtaa
     2281 ccttattctc ttttatatcc atatttattt tctttaatgc ttgcttatca ccataaaata
     2341 aatccaaatc ttttacactc atttttatct tatcgattaa ttccattatt cacacctctt
     2401 aaatttaata attagcttta tttaatttct tagcaacaaa tcttgctagc atgtttagtg
     2461 ctaatacaat tactattaaa acaattccta tactggctgc tgttgctata tctccactct
     2521 ctttagtcag taagtatgag tgtacagtta atgttcttgc actatcaaat attccacctg
     2581 gcatcttggc tacagtacct gctgtaagta atattgcagc tgattctcca attatacgac
     2641 caactgataa gattactcca gataaaattc ctggtattgc acttggaagt actaccttat
     2701 ataaagtttg aaattttgta gctcctaatg ccaatgatga ttctctataa gcttgaggaa
     2761 ctgtttttaa agcttcttct gttgtacgaa ttattactgg taatattatt attgcaactg
     2821 taagtgaacc tgcaactatg gaatatccca tttttaatgt aactacaaag aaaattcctc
     2881 caaataatcc atatactata gatggtatcc ctgataaact ttcagttgca aatcttatag
     2941 ctgtaactgc ttttcctttt ttagcatatt cttgtaaata tatagcagcc aatattccta
     3001 taggagttgc tacaactata gaaactacta ctgtatataa tgttgtaatt atcattggaa
     3061 gtataccacc atcacctgat gctgaataat tgctaaataa gaaattaaaa ttcatgttgc
     3121 caattccctt tataaatata aatcccacta taactactaa tgatactatt gtaaatagtg
     3181 ctgatagata tactaatgac ttcaatagat tctctttgaa ctttctcatc ttaatttacc
     3241 gccttatttg aaagtttatt taaaactaag tttaaaatta atataaatgt aaacaatact
     3301 actccagttg caaatagcat ttcttgatgt gtaccaaatg cgtatcccat ttcaagtgct
     3361 atattagttg taagtggtct tacactatct gttattgcag ttggcataac tggtgagttg
     3421 cctgcaacaa gtattactgc cattgtttca cctaaagctc ttcctactcc taacactact
     3481 gcggctaata ctccagattt tgcagcaggt aatacaactt tgaatattgt ttctattttt
     3541 gatgcaccaa gtgctaatga accttctttg tatgcttttg gtactgctct tattgctgtt
     3601 tctgatacag atattattgt tggtaacatc attactgcta atactattac tattgctaac
     3661 aaactttgac cttttggaag attaaatata ctttgtatag ttggaactat cactgcaaga
     3721 ccaaatacac catataatac tgaaggtata cctgctaaaa gttcaactgc tggtgacatt
     3781 atttttgcta atcttttagg tgctacttct gctataaata cagaggttaa tataccaaca
     3841 ggtactccta taacaagtgc tccaagtgtt gcaaatattg atgctactat catagatgat
     3901 attccaaatt tattggcaga aggaacccaa tctgcaccag ttaaaaagtc tataaatgaa
     3961 tatccttcaa atataaatgg tcttaatcct ttataaaata caaatcctat tatcaaaagt
     4021 aaacttgcaa ctgctactaa agcactaagt agaaaaatat tttttgctac tttttcaatt
     4081 acgtattttg tcttattgcc attattgtca tttttttcta aacctactaa tctagatttt
     4141 actatcataa taccctccca ttaaaatata cttatcgatt ttcgttctgt attcaaaatt
     4201 taatttttta taattcgtct tcatcaaatt tcctgtactt ttattctact ttttttaagt
     4261 taaatctata ttaggattaa gttaagcaag tgtaaaatta tatttacttt ttaacattaa
     4321 ttttactttt aaaactatat ttgacattta ctttacaata tactgttgtt tctctatatt
     4381 aaaatagcag atatagataa taatacagtt tatgtataat ttattaccta tatctgctat
     4441 taagatttgt taactaaatt taacttttat actacatata taattattgt atcctctagt
     4501 ttatatcact attatttcca tcaaattgtt acgatacttg atgtttgcaa taggtatgaa
     4561 tagccaaaaa tataaaaata ggtatttcta tcactgagaa aaacagtata atgtgtggtg
     4621 tccaaaaact catacctcct aagtgtaaga aattaaatga tgtcatttga tataataaac
     4681 taatcttaaa accaattccc cctggcggta ataggtatcc aatccaacta acacctattc
     4741 ccataggaat taagcaaata gaaaaagcaa tcagcataga agtaagagaa tccttacatt
     4801 ttgcagataa aaataatgtt aaactaacca ttgcaagtat tgaaattaga ccaccaatag
     4861 ctataagaat ctgtgcttgt agtaaattta tatttactag actaatgaca gaaaacaaca
     4921 tttgaaatga ggttttcaaa cattcagtac caaaagctaa atcagaaatt gccaaataga
     4981 tagaaataca tataacaaac attattataa atatagaaaa tagagctaat attttagtaa
     5041 acgctagaca aatatgtcca tgtttcgtac aacgaaggat gctatctgat tgtgtctgat
     5101 actcaccaga aaaagtaggt gcagcaattg caacatatat aattaataat ataaaaatat
     5161 aaaatattat ataatcgaaa gcatcttctg gatttattaa gaaattctta atcttattat
     5221 ttaactattt tattatttaa tatatatt
//
LOCUS       DAYAOY010000175         5151 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYAOY010000175 DAYAOY010000000
VERSION     DAYAOY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.180"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(164..364)
                     /locus_tag="ACGI6B_003856"
     CDS             complement(164..364)
                     /locus_tag="ACGI6B_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9753262.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(589..765)
                     /locus_tag="ACGI6B_003857"
     CDS             complement(589..765)
                     /locus_tag="ACGI6B_003857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753263.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(819..908)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1088..1288)
                     /locus_tag="ACGI6B_003858"
     CDS             complement(1088..1288)
                     /locus_tag="ACGI6B_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9753264.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1290..1640)
                     /locus_tag="ACGI6B_003859"
     CDS             complement(1290..1640)
                     /locus_tag="ACGI6B_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HFG9753265.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1802..1990
                     /locus_tag="ACGI6B_003860"
     CDS             1802..1990
                     /locus_tag="ACGI6B_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753266.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2467..2667)
                     /locus_tag="ACGI6B_003861"
                     /pseudo
     CDS             complement(2467..2667)
                     /locus_tag="ACGI6B_003861"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3026..3514)
                     /locus_tag="ACGI6B_003862"
     CDS             complement(3026..3514)
                     /locus_tag="ACGI6B_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9753267.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4318..4692
                     /locus_tag="ACGI6B_003863"
     CDS             4318..4692
                     /locus_tag="ACGI6B_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753268.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4797..5151
                     /locus_tag="ACGI6B_003864"
                     /pseudo
     CDS             4797..5151
                     /locus_tag="ACGI6B_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2074 a          627 c          586 g         1864 t
ORIGIN      
        1 tacatacttt ataaatatat ttttgttaga tttattttat aaaaagtatt tagactaagt
       61 attattttta agtattttct tgttgaagtt attttttcct tttttacaaa attcgtaaat
      121 aaaaaaaaga ctcttaaaag agtctttccg aaaaatctat atattataat atagttatat
      181 tttctgcttg aggacctcta gcgcctttaa ctatatcaaa gcttactttt tgaccttctt
      241 ctaatgattt aaatcctgaa gtttgtatag ctgagaaatg agcaaatacg tcatctccac
      301 tttccattga tataaatcca aatccttttt cattattaaa ccattttact attccattat
      361 tcattaaaaa tactcctatt aacctatatc aagatatact tgatttactt gtgtattacc
      421 acaaatatta ttgtatcata gattaacata aatgtctatt taattataat ttttagttaa
      481 catttttatt tattcttaat atgaaaataa atttaaacat tttatattac caatatatta
      541 atgtaaatgt agataaaact aaaaagggtt ttctagaaaa aaatctgtct aattatactt
      601 acttaccaat accatcactt gatgcaagca agattccaca actttttctc cgtactctaa
      661 tataaaatca tctaactgtg tatctataaa atcacaataa tcgtatgaat tactaatata
      721 agcagttaac atatgtgtac atttatccat tttatctcta aacataatgt atttatcccc
      781 ctaaaaaact ttcatgaaaa acaaaaagaa aatatctttt tggcaactgg ctgacataac
      841 ttattttata agcgttagtc cctatagctt tgcgtcacta aatttctcta gttttgccga
      901 tttagtttta ttctacagat taataataca atagaattag ttatcattca acatatctag
      961 ttgaataatt aataaattta tatatgaact ttaaattatt tgaaaaaata aaaagcccct
     1021 ctgtcaggag tgacttaaaa attgaattta agtttgtatt taaaacacat ttttaataat
     1081 gcaaaaacta atcaaaaata aacaattcat caataggtgt atctactatt tttgatatat
     1141 ctattgctaa tttcaaagaa ggattatact ttcctgcttc taaacgcata atggtttctc
     1201 ttctaactcc tactttttct gctaattgtt cttgagttaa ttcttttaat tgtctatatt
     1261 tttttaggtt acaagtgaat ttaggcatat tatttttcct ctaaaatata aagttttata
     1321 gcataaacat ttaaagtgat tgcatatgta attgatagta aaacacacat taattctata
     1381 ttcctagaca ctatagtaat acaccataaa atgaataatt ctataatagc aagctgtcct
     1441 gtaaatgcca tagctgcttt ttcatcttgt acataccttt catctgcttt atcaccattt
     1501 atttttgcta taataaagtt tgagaaaaaa gcaaaaaatc caatatatgt aaggtcagca
     1561 acattgccag atgaaaaata tctaagactt aaaaatccaa ggaaaccaca aaaacccata
     1621 aaaaattttt tctgaatcat tttttatcct ccttagtaat tttaaaagag atatatttat
     1681 atctttatga tacaaatata tctcttttat attcttttgt caatagaaaa tcagtattat
     1741 acaatattta tactgcacca taattgttag agcaaaaatc taatgcttga gatgtttatg
     1801 tatgactaaa tatgatttta atttcaaatt aaaagtagct aaattttatt tgaatagaga
     1861 aggaagagat attttaatag taaaacaata tggagtttat gaccattctc aagttgaaag
     1921 atgggttaat atattaggag agagtgaact aaaaataaaa aaattttcta tactatatgg
     1981 ttcaagttag atgttataaa ctatatgatg ataactaaat atagtgtaca ataaatcgct
     2041 aagtattttg gaataaacaa tagcacagtt atagcaacat gaaaataaaa atttttacat
     2101 agtggtgttg atgccttttc taaaatgaaa ggaagaccat aatgaataaa aacaaaaaca
     2161 ctaaagaaaa aataaattaa caagagaaca agaattagaa tatgaaaata aatttttaaa
     2221 agatgagctt gagtttttaa aaaagatgtg tgtctcaggg aagccacatt aaaaaacagt
     2281 ggtatcatta ttaatcaaaa gaaagttaga tgtattatca aaaaattaga acttaaatgc
     2341 atagcatttt ttcataaatc acataaatat aactcctata aaggtataat tggaaaagta
     2401 aaaaataata aaacaaatag aagatttaaa acatcaatag catatcagaa aataacaaat
     2461 gatatgctag aatttataaa atatatctaa tgataaaaat ggaaaatcaa ttataaaaaa
     2521 ggcatatctc gattcatttt ttgttatata gtcaaataag aatactatag atgaaagata
     2581 cgattttgca tatgtattaa gtaagtattc tgatgttttt ggttgataca catgcaataa
     2641 agaagttgtg tttgcagata aattagcaaa actatagtat aagttgcaac aataaagaaa
     2701 atgaaaatta tcatactaaa gcataaggca tgttacaatt ttattagata ggacattaac
     2761 atacactaaa gtacttttta ttataaagag tacttatttt tttgaaattc attaatgtat
     2821 aaactatcaa gaatattact caacataatt taaaaacaac ttgaataggt atttttaaat
     2881 tatgtgatat aataaaaaat agaagtgtat gccaatacac ttctgtagtt ataactaagc
     2941 attctattct tgaggagtgc ttttttcatt tttataacca actcttttta ctttaacaat
     3001 aattatcaga cctatatatt tatttttaat tgtctgattt attagataaa aaagttatta
     3061 tcatattttt atcatttggt atttttattg aactaccata atcttcttct aatttatcta
     3121 gaccactttt acttatttct ccatctataa ttatgtttaa ttctaagtat tcttcactac
     3181 cattagatga cactaaagtg tacgaaccat tataaggcat gcctaaattt tctattttat
     3241 gtgccaaagg gtcttcatca ccaataccag agtttactgt tatttttcca tcttttttag
     3301 tccaccatat tatgtttcct tcagtatatt ttgattcatc tgcatagtag ctaagagacg
     3361 cactttttat tgcacttata tcagcttcaa gtttagctat ctttgctttt tctatatttt
     3421 taaataatgc tggaagtgct actattgcca atattcctat tatagcaatt actactaata
     3481 attccactag agtaaatcct tttttatttt tcatttattt cccctccata taaaattaat
     3541 aatatataaa tttttatttt gttaatttct actttctata ttttaccata tatagacaaa
     3601 aaaatcactt tctatgacaa atatactatt attttacaat ttatggaaat gatttaaaat
     3661 gacatttctt gttagaaaag atataagaaa tggattaaaa taaagaaaac tacagtcata
     3721 tcaatgtcaa taatattatt taaaagcaga tgaaaataaa aagaggactc agaaaataat
     3781 gtctatttaa ttatacttac ttaccaatac cataacttga tataaacaag attatactac
     3841 atttttttat gctctagtat aaaatcatct aactgtgtat ctataaaatc aaaataatta
     3901 tatgaactat cagtataata agtcaaaata tgtatacatg aatacatatt ttaaaagata
     3961 tatattatta catacctaaa aaactttcat aaaaacaaaa ataaaatatg cttttagtaa
     4021 cttgttaaca taacttataa tttattttat aagcattagc ctatatagct ttgtgctact
     4081 aaatctcttt gattctaacg atttagtgct attctacaac taaaataata caatagagct
     4141 atttattgtt tggtagcatt gtttgtaaat taaacaaatt tatacatatg aatattattt
     4201 tacattcaaa aaaacagact aaatatgtag aatatttggt aaaacttacc tctagttttt
     4261 gtcttaaaaa gctcctatta ttaacttaag ttagaagaat taaggaagtg acataaattg
     4321 aaagaagaaa tattaataga aaaattatta agagcagaat taatagtaat ggagtattta
     4381 tggaaaaaag acactctaat gcctaaaaaa gaaattgtaa aagaaataaa acagatttat
     4441 ggatggcata aaagtacaat aaaaatacta ctaaaaaggc tagttgctaa aaggtatttg
     4501 gctagatata ttataaattc tcaatctcat tataaaataa tagatagtaa agaatattat
     4561 acttttaaga aaaaggtact aaaatctagt aaaagcataa aaataatgcg ttcacttacc
     4621 actacacata aaaatgtaag taaagagaag ttagacctat tagatgagta ttatagaaac
     4681 ttaaaagaat gatatagtaa aaatcgaatg taattattta ccttaattct tttgtttgta
     4741 aaactttatt gatacagaat ttaacatata cataaattgg taaaggaatg tatactatga
     4801 aaattagtaa attaccagaa gcggaattaa aagttatgag atatatttgg gaatcagaaa
     4861 aggtattaat atcaagagaa atcaccaagt ctatggaaca aaaatatgaa tggaaagata
     4921 caactatatt tacagtatta aaaagattac taaagaagag agtttttaga agcggagaaa
     4981 atagataagt gtacacacta taatgtatta gtaaaaaaaa ataatatcag agattttaaa
     5041 caaaagaatt tttaaagaat acacataaaa attcaatatt aagtttaatg tcagcactat
     5101 gtagtaagga tgatgaagta gatggtgata ttttaaattt attagaagaa t
//
LOCUS       DAYAOY010000176         4958 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAYAOY010000176 DAYAOY010000000
VERSION     DAYAOY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.181"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(30..467)
                     /locus_tag="ACGI6B_003865"
     CDS             complement(30..467)
                     /locus_tag="ACGI6B_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFG9753269.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(724..873)
                     /locus_tag="ACGI6B_003866"
     CDS             complement(724..873)
                     /locus_tag="ACGI6B_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HFG9753270.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(886..1116)
                     /locus_tag="ACGI6B_003867"
     CDS             complement(886..1116)
                     /locus_tag="ACGI6B_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HFG9753271.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1975..2196)
                     /locus_tag="ACGI6B_003868"
     CDS             complement(1975..2196)
                     /locus_tag="ACGI6B_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HFG9753272.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2776..3033
                     /locus_tag="ACGI6B_003869"
     CDS             2776..3033
                     /locus_tag="ACGI6B_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFG9753273.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            3502..4740
                     /locus_tag="ACGI6B_003870"
     CDS             3502..4740
                     /locus_tag="ACGI6B_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFG9753274.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1896 a          498 c          693 g         1871 t
ORIGIN      
        1 tgatattaat aaattacttt agatttttat taaatgttta gcttttcttt aatatttttt
       61 atataacgac gatttataat aactttttcg ttgtttttta aagtagcttg catgcgatta
      121 tgtgatatta cttttatact ttttatgtag tccatattta aaatcatgga tttagatatt
      181 cgtgagaaag atgtattagc taataatttt gataatttat atatttttaa atcaatttca
      241 aatacatcat cttcataata agcaaatgtt ttatttccaa ctgtctcaaa gtataaaata
      301 tcacagacat tgagtttata aatctctcct gattttttac cttttatatc aagtaacggt
      361 atactcaata atgaaattat tttttcaatg ttattatcta gagcagaaca ttttatggtt
      421 atttcaatat catcacaatt aataccttgt tcgacatgta ttttcattaa tcatcacctc
      481 attttattac tgtcattaga atagcattat atcttattga gtccaactag atgcgttcta
      541 aatttcaaat taagcatagt gaaattcaaa aataaaacag tgaaattcaa atttgaggtg
      601 gtaaaattca aaaaaatact atggatattt ggataaatta tccaaatatc ctagtatttc
      661 acatagaata tgtctaatta ttttacaaat agacctattt tatgacttac atatattgtc
      721 aaattacttc ttaaatatca aaatattttt ggataatgta tcaatactgt ttgataaagt
      781 atttaattta ccctcaattc taaccaacaa gtacatggat agagctatag gaaaaccaac
      841 attggttatt aacagttgta aactatcatc cataaaatta gccccctatt ctataactaa
      901 gtcataagga gtggtatttg tttccacaac cttggcttca acaacagaag ctaggtcaaa
      961 tcctccaacc gaaaaaatat ttttagatac tatcaaatcc atgacagatt ttatattttc
     1021 ttcagttaaa tcatcctttg gattttctat agaaaaagaa acttgtttat cctttacatt
     1081 tttaaagctc atgactaatt ttttcttaat ttccatataa ttacctccga tataataatt
     1141 tatattttaa ttgtttgctt gttaatattg gtattttaat tatcaatatt aatgttttgc
     1201 ttgttagtat taatatttca attgtcaata ttaatgtttt gcttgttagt attaatattt
     1261 caattgtcaa tattagtgtc ttgcttgtta gtatcagtgt ttcaattgtc aatattagtg
     1321 ttttgcttgt tagtattaat atttcaattg tcaatattag tgttttgctt gttagtatta
     1381 atatttcaat tgtcaatatt agtgtcttgc ttgttagtat taatatttca attgtcaata
     1441 ttagtgtttt gcttgttagt attagtattt caattgtcaa tattagtgtt ttgtttgtta
     1501 gtatcagtgt ttcaattgtc aatattagtg ttttgcttgt tagtattagt atttcaattg
     1561 tcaatattag tgttttgctt gttagtatta gtatttcaat tgtcaatatt aatgttttgc
     1621 ttgttagtat tagtatttca attgtcaata ttagtgtttt gcttgttagt atcagtgttt
     1681 caattgtcaa tattagtatt ttaattgttg actctagtta ttgctgatat agaaaatatt
     1741 taatttattt caattaatta gattaaagta gggtagcatc taaaatagct aacatgttta
     1801 agttgaaata cgtttaatat attaattttt caaagcgtta atatattaaa gttataacca
     1861 aaattgaagt tataatctgc caaatctata gcgtattaag tgcattatat aacttaatct
     1921 tttatataaa gagttaattt tttaatttgt aaagcagagt acgtttaaaa gacactattt
     1981 agatagtgat gtactatcta attttaaaat atctacaata gggttctctt gaagacttgc
     2041 tattgcttct gcaacagcat aaacatcttc atttaatgcc tctgctttta catttgcaaa
     2101 tgatttactt ttaacaactg ccttaccctt atcatcaaaa ccagcatcca attttagttt
     2161 tatactagat aaattttttg ttgaaacaac ttccataaaa ttacctccta aaaatttaat
     2221 ttcaaagatt tgtcgacttt ctttgttagc tataatacta tcatctaaga taatatcgga
     2281 gttaaacagt aatttctaaa gattttttgt gtaaattttt ataggaaaaa ttttactttt
     2341 ttgctgatga aaaagtttac aagttatttg tgaagatatg ttcttagtgt tttgaaatag
     2401 tatttgtata tattcatgct tagttgagat agttttgtat ggattttcat atgtgtagag
     2461 ttaaatgtta ggtactaaag tttatgttaa attaaatgtt aaagcttatg ttaaaaattt
     2521 tattaaattt aatctgtaaa taaaaaagtt aaaagtccaa tattaacatt ttacttttaa
     2581 accattaaaa tctttattaa agctaacaaa gttataagtt atgagaaatt ttattgaagt
     2641 taatacaaaa attaatttta aagctaagaa attttttatt agattcaata aaactaataa
     2701 atatagttta gtcaataatg tattaaataa aaactttgtt aagtctataa aaatatctta
     2761 aaggaggtgt aacttatgaa gataaaagtg gaggatatac cagttcaatt tcatgcaatg
     2821 gtagaaataa tcggaattga taagttcata gaggttgcca agttgtatgg aggaacaaat
     2881 acttatatac ctacgtataa ggggcttttt agatatgcta gaaataggga tatagtaaaa
     2941 caatttaatg gagtcaacca caatgaattg gctattagat ataatatgtg tgtaagcaat
     3001 attaagcgaa tactcaatga aaatagtgtt taaatataat aaaataatgt ttaaacataa
     3061 tgaaaatagc cttgttaaac gtaagagtaa agacaaagaa gatgttttat aaagtttttt
     3121 tagacaaatt aactaaattt actacttatt aatttaaata cattgtatat tctataaaaa
     3181 aatttgttaa atactggaga tatattgaga atacaaagaa aatggtcgaa aatatttgtt
     3241 ggctaaaaaa tagataaaat gatacaattg cttgtataaa ggggaaaaat aaataaataa
     3301 actatataaa taaagaaata gcaattaata tgacataata tatttataaa attaagaata
     3361 taaatagata ttttaaggga ttagttgtta aattaggaaa tattaaaaaa aatatatatt
     3421 tatatttaaa ctattttgtt atttatataa aaatacttat ctagttacta gcaattatta
     3481 gtagaaatgg gggaaataga gatgaataag ggattttaca aattaaattc agtggtattg
     3541 aactttgata ttaagaatga tgttaacaag ataagattta atcattatat aatgagaaaa
     3601 gaaaacatac aaagacttca aggaactctg ccaaacggac aattttatat gacagtgaga
     3661 agagctgcta cagatttaca actttctatt tctactataa gtaggcttgt aaaagagttt
     3721 ttggatttag ggataataag attagtgtct aagggtgtaa aaggtaatcg ttctgtatat
     3781 agttacgtaa gtatagagtt taatggaagt ttatttgatg atagtaaata tatggaactt
     3841 gactgtgtat gtaatgatga atttattgat aatagtggca tagagtttga ttgtgatagc
     3901 aaagataatt cttttaatgg agatataaat gtagaaactg tgcattgcat taatgagtat
     3961 ttctctgatg atgatgtaga gcataattat aacaatttcc ataacaaaga tactataaag
     4021 gataaagcca tttctaatat tagcactatc aaaaaatata ttgatgaatc ttttgataca
     4081 aataaatcag aaaattctaa cttacaacag gagtcaataa atttcaactt taatgataca
     4141 tgcaatgaaa tagataaaag aacgaataat agtatagaaa ataatcaaaa taaaaggcct
     4201 atgagattga gtgacatcag tcttttaagt gataaaggta tgacaaataa aaagaattac
     4261 tttaatcata atcatggtat acaaaaaggg actaaatctg taacaaagaa aaaagaatcc
     4321 ttaaataaat ttaataaaaa aaatatacag caaagcatta taaatatgct aaataaaaaa
     4381 tcaggtaaga attttaaaat cgattctcca ataagcataa aacttatcaa tgaaaggcta
     4441 aaggaaggct atgtcttaga agatttctac aaagttattg aggtaaaagt ggctaagtgg
     4501 aaaaatacca taatggaaat gtatataaga ccagaaactt tgtttagcta taagtttgaa
     4561 tcctatgtaa atgaaaacat atctatgaaa agtcaaagta atgttcaccc atgtaaagaa
     4621 ggcaatagta atgatagcag taattatata cattctgatt atataacata tgatggcagt
     4681 gtgcgtgaga gaaaaaaata tgagaatgta agatactgga atcctatatg tgaaatttaa
     4741 ttagtttaaa aatgtattta tgataaataa gatattaata gagaatagtg agctatagca
     4801 aatgatatat caatagttgg tagtaagtta tattaagtaa gatattacta tcaggtagtg
     4861 aattatattg aacgatatat taataattgg cagtaagtta tatcaaataa gatattacta
     4921 tagagtaatg aattatattg aacgatatat taataatt
//
LOCUS       DAYAOY010000177         4900 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYAOY010000177 DAYAOY010000000
VERSION     DAYAOY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.182"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1403
                     /locus_tag="ACGI6B_003871"
     CDS             <1..1403
                     /locus_tag="ACGI6B_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HFG9753275.1"
                     /translation="MGTYYSMYGGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYYP
                     STGGAGGGLEGGTGYSSNDRYRPGGTQYQGGIGRVNTENGSFGKGCSVKDSTGEGGGG
                     GWFGGAGMNGVGAGGGGSSYVLTKDSYKPTGYTPTSEYYFDNIVMTPGGNTAGAYGYA
                     QITLLQSLPFLNISSYNSTQATFKADHTDPTLLTKIEYFIDDVLKETITTDLTEEKTI
                     NYTLEDNALHTLKIVVTDSNNATAEKAVSISKNIMPLPENVNLNDISTKLVEVNAGFK
                     TGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQL
                     NSKYKVASGNSVVNYCTNTKGFYFNSDYLFLFPGAIQINGLNFVPNIFFTTFELIDDG
                     YFHKYFVFACRGIFTQDFVITAHYYRLTSYLQDFKVGGEVLKLNERDVYMDNRGIQLP
                     CSRQGSSFKWQAIKFI"
     gene            1420..1713
                     /locus_tag="ACGI6B_003872"
     CDS             1420..1713
                     /locus_tag="ACGI6B_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753276.1"
                     /translation="MDRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGS
                     IDYTRNRIIGINIETKEPILEEIPVFISEEEKRIQELENQILLNENKKVGGIL"
     gene            1713..1895
                     /locus_tag="ACGI6B_003873"
     CDS             1713..1895
                     /locus_tag="ACGI6B_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753277.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIRESGVVEEAEPTI"
     gene            1928..3160
                     /locus_tag="ACGI6B_003874"
     CDS             1928..3160
                     /locus_tag="ACGI6B_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753278.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKSNVVTALNSKGADVTTSDTWEEIKNKIDIKEGRLDLRETTLSNNYSYLVTNGA
                     IKYIERCSGNFKTFEYEEPYFYVIKETHLIKINAIDETVVFDITLANANFSCICVTQE
                     YLFISDNTKLYKINKITGIEVQSIEGAYYKLCTYGEFIYGVYGDETSSTLHKIRISDM
                     YIMLTKNLVSNGIYNFKGGKFVCNKNAIYATTEHSNSSSITTCHLSKINFDFAIAKDF
                     RIGGYLHMKNIKFLNDFVIVSDAERGIEIDSNKKSALVKYDANLNLIAYSDDSKYDNF
                     DIYNGYIYAIYSPSSSPFVKISLNTLKHIDSYRKLTETYPNAGMFVINDIVFFISSGI
                     FRNILSKKVYSDEKGESL"
     gene            3157..3456
                     /locus_tag="ACGI6B_003875"
     CDS             3157..3456
                     /locus_tag="ACGI6B_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753279.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIESLPT
                     QKYAENKEAKLFINIDTKEVFYRYTDIKSNIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            3458..3595
                     /locus_tag="ACGI6B_003876"
     CDS             3458..3595
                     /locus_tag="ACGI6B_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFG9753280.1"
                     /translation="MNWYKIIADFYNNGNWTKEQVKTAVEKNKITASEYKEITGEDYI
                     A"
     gene            3627..3857
                     /locus_tag="ACGI6B_003877"
     CDS             3627..3857
                     /locus_tag="ACGI6B_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFG9753281.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            3877..4134
                     /locus_tag="ACGI6B_003878"
     CDS             3877..4134
                     /locus_tag="ACGI6B_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9753282.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            4134..>4900
                     /locus_tag="ACGI6B_003879"
     CDS             4134..>4900
                     /locus_tag="ACGI6B_003879"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9753283.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKNEEKTYKIPRVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKGLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKFGYEGIAKLIVEGILNKNINNKEDSEGKIMYKHTIVYDGEVDKITATVVG
                     WGYNDGKILICDIKDYVPGQTQNLYVVGGGACEKIGS"
BASE COUNT         1919 a          536 c          939 g         1506 t
ORIGIN      
        1 ttatgggtac ttattattct atgtacggtg gtggtgctac cgatataaga cttgttggtg
       61 gtgcttggga taatgagcaa ggtttgctat ctcgtataat tgtcgcaggt ggtggcggag
      121 gttcatatta tccttccact ggtggtgcag gaggaggatt agaaggaggt actgggtata
      181 gttctaatga cagataccgt cccggtggta ctcaatatca aggtggtatt ggtcgtgtaa
      241 atacagaaaa cggaagtttt ggaaaagggt gttctgttaa agattcaact ggcgaaggcg
      301 gcggaggtgg ctggtttggt ggtgcaggca tgaatggtgt aggagcaggt ggaggtggaa
      361 gtagttatgt attgactaaa gacagttata agcctactgg ctacacacca acatctgaat
      421 attattttga taatattgtt atgacaccgg gtggaaatac tgctggtgct tatggttacg
      481 cacaaataac tttacttcaa tcattaccat ttttaaatat atcatcttat aattctacac
      541 aagcaacatt caaagctgac cacactgacc ctactttatt gactaagata gaatatttta
      601 tagatgatgt attaaaagaa actataacaa cagatttaac agaagaaaaa acaattaact
      661 atacactaga agataatgca ctacacacac ttaaaatagt tgttacagac agtaataatg
      721 ctacagcaga aaaagctgtc agtataagta agaatataat gccattgccc gaaaatgtta
      781 atttaaatga tatatctact aaactagttg aagttaatgc aggatttaag actggtaaaa
      841 caagtattat aaacactttg gcactaaaga atatagaagc aagtttaaat aacacattag
      901 tcgagttgtc agagaaaata aaaacaagtt ttgatagttc agacgctagt gtacaggatt
      961 tgatgaacca gttaacacaa gctaataaca ctataagtca gttaaattct aaatataaag
     1021 ttgctagtgg caatagtgtt gttaattatt gtactaatac aaaaggattt tattttaata
     1081 gtgactatct gttcctattt cctggagcca ttcaaattaa cggacttaat tttgttccta
     1141 atatattttt tactactttt gaattaattg atgatggtta ttttcataaa tattttgttt
     1201 ttgcttgtcg tggtattttc actcaagatt ttgtaattac tgctcattat tatcgtctaa
     1261 catcatattt acaagatttt aaagttggag gtgaggttct taaattaaat gaacgtgatg
     1321 tttatatgga taatagaggt atacaacttc cttgttcaag acaaggttct tcttttaaat
     1381 ggcaagctat aaaatttatt taataaatga ggtgataaaa tggatagagc aaatagaata
     1441 atttacgacc agacaggcaa aatactgcta cagacagggg aagcaacagg agatatacta
     1501 gaacatgata caataacaga attacattat attgatgttg agtatggaag tatagactat
     1561 acaagaaata gaattatagg tataaatata gaaacaaaag aaccaatttt agaagaaata
     1621 ccagtattta tctcggagga agaaaaaaga atacaagagt tagaaaatca aattttatta
     1681 aatgaaaata agaaagtagg aggaatttta taatgaatat aaataatgtt gtggtaagaa
     1741 tattagcaga gagaatatta aacggaggct taaacccttt aaaaaatcga gaatttgaac
     1801 tggatgatgt aactaatgca gaatacagaa aagcagtaga ggattatata attagagaaa
     1861 gtggagtagt cgaagaagca gaaccaacta tatagagggt tcttttttat tgaaagaagg
     1921 tgactaaatg acttttaaag agttagttaa taaagttaga aatcttgtat tagaagcaaa
     1981 gaatgtaact atagaagata cagaaaataa ctttacaagt gataatgtag aaggagcatt
     2041 gaaagaggtt tttcaaaatg gagttaatgc taaaagtaat gtagtaacag cattaaactc
     2101 caaaggtgca gatgttacta caagcgatac atgggaagaa ataaagaata aaattgatat
     2161 aaaagagggg agattagatt taagagaaac aacactttca aataactatt cgtatttagt
     2221 tacaaatgga gctataaaat atattgaaag atgtagtggg aacttcaaaa cttttgaata
     2281 tgaagaacca tatttttatg taattaaaga aactcatcta attaaaatta atgctattga
     2341 tgaaacagta gtttttgaca ttactttagc taatgctaac ttctcatgta tctgtgttac
     2401 tcaagagtat ttatttatat ctgacaatac taaattatat aaaataaaca agataacagg
     2461 aattgaagtg cagtcaatag aaggtgctta ttataagtta tgtacttatg gggaatttat
     2521 ttatggagta tatggagatg agacttcttc tacacttcat aaaattagaa tatctgatat
     2581 gtatataatg ctaactaaaa atttagtttc taatggtatt tacaatttta aaggaggtaa
     2641 gtttgtttgc aataagaatg ctatttatgc tacaacagaa cactcaaatt caagtagtat
     2701 tacaacatgt catttatcta aaataaattt tgattttgcc attgctaaag attttagaat
     2761 tggaggatat ttgcatatga aaaacattaa gtttttaaat gattttgtta ttgtatctga
     2821 tgcagaaaga ggtatagaaa ttgatagcaa caaaaaaagt gctttagtaa aatatgacgc
     2881 aaatttaaat ttaattgcgt attcagacga tagcaaatat gacaattttg atatatataa
     2941 tggatatata tatgctatat attcgccttc tagtagtcct tttgtaaaaa taagtttaaa
     3001 tactcttaaa catatcgaca gctaccgaaa acttactgaa acatacccaa atgctggtat
     3061 gtttgtaata aatgatatag ttttctttat tagtagtgga atttttagaa atatattgtc
     3121 aaaaaaggtt tattcggatg agaaaggaga atcattatga tttatttagg aaatttaatg
     3181 gatacagaag aacaaaatat aaaatatgtt ggtatgatac actatgaacc aaatttgtta
     3241 tcggaggaaa acttaaaaca aggtattttg atagagagtt taccaactca aaagtatgca
     3301 gagaataaag aagcaaagct atttataaat atagatacta aagaggtttt ctatagatat
     3361 acagatatta aaagtaacat agaagataaa gtaaattcta cagaacaaac aatagcagat
     3421 ttaacatttc aattaatgag taatggggtg atatagtatg aattggtata agataatagc
     3481 agatttctat aataatggta attggactaa agagcaagtt aaaacagcag tagaaaagaa
     3541 taagataaca gcaagtgaat ataaagaaat aacaggagag gactatatag catagtcttt
     3601 tttaattcaa aaattaggag gttttcatga atgaagaact tttcgaagca gatttaaaaa
     3661 gacatgaaac aagaataaat aaacatggag aagaaataga cgaattaaaa atagcaaata
     3721 tagagtctaa agcagagtta aaagcattgt gtgagaatct aaactcactt acaagtatgc
     3781 tcaaatggct aattggtaca atgattacaa cacttgtagg gttctttata tttgcagttc
     3841 aaagaggaat attttaatta attaggagga taagagatgg ataatttaat aagttttata
     3901 ccagagcagt tgctaatttt agtagctgct ctctctatta taggtaaggg ctgtaagaag
     3961 tataagcaac tagataacaa atatattcca gtagtgttat taatacttgg tataggattt
     4021 tctatttgga tgttaggact aagtcctgtt gcagtcttac aaggcgtgat ttgttggggt
     4081 atatcaatag gtataaacca aacttacaaa cagttgaagg aggaaaataa ataatgaaaa
     4141 tatgtataac agtaggacac agtattttaa aaagtggagc atgtacttct gctgatggag
     4201 tagttaacga gtatcaatac aacaaatctc ttgccccagt attagcagat acatttagaa
     4261 aagaaggtca taaggcagat gtaataatat gccccgaaaa gcagtttaaa actaagaatg
     4321 aagaaaagac ttataaaata cctagagtta atagtggagg atatgattta cttatagagt
     4381 tgcatttaaa ttcaagtggc gtaggagctt ttggaacaga agtattttac tatagtgaaa
     4441 aagggaagga atatgcgcag agggtagtag ataaactatc taaacctttc ataagaaaaa
     4501 aaggagataa agaagtaggt aatagaggtg ttaaattaga taaaggatta tatattttaa
     4561 atagttccaa acctactgca atactaattg aaagtttctt ttgtgataac aaagaagatt
     4621 atgagaaagc taagaaattt ggatatgaag gtattgctaa gttaattgta gaaggtatat
     4681 taaataaaaa tatcaataac aaagaagata gcgagggaaa aatcatgtat aaacatacaa
     4741 tagtttacga tggagaagtt gataaaatca ctgcaactgt agttggttgg ggatataatg
     4801 atggtaaaat actaatatgt gatataaaag attacgtacc aggtcagacg caaaatcttt
     4861 atgttgtagg cggtggagca tgtgagaaga taggttctat
//
LOCUS       DAYAOY010000178         4815 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYAOY010000178 DAYAOY010000000
VERSION     DAYAOY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.183"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            170..1087
                     /locus_tag="ACGI6B_003880"
     CDS             170..1087
                     /locus_tag="ACGI6B_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9753284.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1216..2145)
                     /gene="pfkB"
                     /locus_tag="ACGI6B_003881"
     CDS             complement(1216..2145)
                     /gene="pfkB"
                     /locus_tag="ACGI6B_003881"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFG9753285.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            2414..3139
                     /locus_tag="ACGI6B_003882"
     CDS             2414..3139
                     /locus_tag="ACGI6B_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9753286.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3222..4376
                     /gene="nagA"
                     /locus_tag="ACGI6B_003883"
     CDS             3222..4376
                     /gene="nagA"
                     /locus_tag="ACGI6B_003883"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFG9753287.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
BASE COUNT         1799 a          632 c          686 g         1698 t
ORIGIN      
        1 tatatcaatc aattttatgg atatcctaca aaattacttt atcaatattt cttatttcgc
       61 taatttactt atataagctt taaatttatt ttttgataca aagtttacaa atttatgtgt
      121 ctgaattata atcaattctg taaatataaa aaccttagga gaaagtatta tgaaaaatat
      181 aaaaatggga atgataggtc ttggctccat agctcaaaag gcatatttac ctatattaac
      241 taagtctgag agatttgagt ttgtaggagc ttttactcca aataaagtta aaagggaaaa
      301 agtctgtagt gattatagaa tcatgccatt cgattctatt gaatccctag ctaaaaaatg
      361 tgattgtatt tttctacata gctctacaga aacacactac gaaattataa aaatattatt
      421 aaatttaggt gttcatgtat atgtagacaa acctcttgct tcaacagtaa gtcaaggaga
      481 agaacttatc gagttaagta cgaaaaaaaa cttaaattta atggttggat tcaaccgtag
      541 attctgtcct atgtacaaag aaattaaaaa taatgctaca gaaattgtat ctatcaatat
      601 atgtaaacat ggattaaatt ccttaagaaa tgttagattc gacagtactc ttatagatga
      661 ctatatacat gttattgaca ctgctttatg gttagcaaat gaagatgttg aaattagtgg
      721 agaggattta tttttaacag acaataagaa tcttatattt gtaagtcata aacttaaagg
      781 taaaaatttc tctataaaca cctctatgca tagagattct ggaacaaagt tagaacaagt
      841 tgaaattctt tcaaagggta agattcaaag agttaagaat cttaatgtac tggaaataga
      901 agaaggtgga aacctaactt taaagcaaag tggtgcatgg gtaaatatat taaaacaaaa
      961 gggttttgag gatatatcaa atcattttat tgattgtata gaaaataata taaaaccagc
     1021 tattaatgga gaagagtgta tcaaagctca aagattacta gagaaaatta taaattcagt
     1081 aaaatagata tttattataa aaataggtta aaaatgaatt tttctaacct atttttatat
     1141 aagactatat cttttatatt aaaattaaaa tctattacaa caacattaaa tttattatat
     1201 tcccattaaa tgtatttaac ttaacttttt tacttcaact aaatcttgat atttatcaat
     1261 ttctcccaaa tctacaaatc cagtttccat atttaaagca ttacttatac cacaagcatt
     1321 tgcaaattta agtaaatttt ctattttata accccttaaa atacctacac taaatccagc
     1381 cactgtgcta tctccactac ctacagtatt tatacacttt acaactggta cttttacatc
     1441 atatacatta tcttcgttta aatacaccat accatctttt cctaaagata tacatacatt
     1501 ctctgcaccc atttcaataa gtttttttcc tgacaagata atttcctctc ttgattctat
     1561 attcattcct aataaatgtc ttatttcatc tatatttggt tttattaaat atggttttga
     1621 ttttaaagct atttttaatg cttctcctga tgtatctaat ataaacttta tattatttgc
     1681 tctacatata tttcctatct tttcataata gtctatcggc atattctcac aataactacc
     1741 agaagctacc agtattttag tattttttag tacttcctct atatcatttt caaatctctc
     1801 taaatcgctt ttttctacta ttggtccttt ttctaaaaat tctgtactaa ctttattgtc
     1861 ctcaattatg tttaagcaat ttctagtttc ctgctctatt tttgtaaatt tattttgtat
     1921 atctagtttt ctaagttcat tttttataaa ttctccatta aatccgccta gaaatcccat
     1981 agctaaagga ttttctttta aaattttaca taccttagct acgtttaatc ctttacctcc
     2041 agcagttgca ttcttactta ctaccctttg cacctcacct atgtttatat tattcactct
     2101 atacattcta tctatagatg ggttgaatgt tattactgta atcatactag attctcctca
     2161 ttattaattt ttacttttct aaacaataaa ttaatctgta aaataaaaaa tacctcatct
     2221 ataatatacc caaagtggtt attaccaatt tgaatataaa ttattttact tgttttgtca
     2281 atgtaccata attttataat aaattaataa ccttacttat tgaattttga ttaaacttta
     2341 ttcattgaca taaataattc tatattctat aatcaaattg tggtaataac cacttagaaa
     2401 ggactatatt tttatgataa aaagaaatga taaaagacca atatatgacc agcttgttga
     2461 gatattaaga actaagattg aaaatgagat ggaaccaaac gatagaatgc tttccgaaag
     2521 aaaaatttgt gatgaatatg gagtaagtag aaccacagtt agactagcta tggctgagtt
     2581 ggaacatatg gggtatatat acaaaagaca tggaaaagga acttttgttg ctgcactaag
     2641 tcaaaattct caaaacttaa tggaaagtta tagttttaca gattatatga gaacacaagg
     2701 aaaaaagcca ggtacaaagg tattatcatt tgaggtagta gaaagtacaa agtatttctc
     2761 tgaaaaatta ggtataactc ctggtgaaaa aatgataaaa ataatgcgtt taagacttgc
     2821 tgatgacctt cctatgatgc ttgaaagaac atatcttcct atgaaagaat ttataggatt
     2881 gacaaaagaa aaaatcgaac aaaaaccttt atatgaaata tttagagaag attatggcga
     2941 gattattaaa gttgctgatg aagaattttc agctggaata atctctgata aggaagctaa
     3001 gttattggaa gtaccaggag attcaccttg tctgaaatta cttcgtacca catataatga
     3061 tgacaatata gttattgaat ttactttgag tgttgctaga agcgataaat ttgtatatag
     3121 agtaagacat gtaagatagt aagttaaata actttcaaga aaatagaatg atattataaa
     3181 cgtcaatgcc acaatgatag tataattata aggaggttat tttgaaaagt tttatttatg
     3241 cagataaatt ttttatgaaa catggaatca aagaaaaagg ttaccttagt ataattaatg
     3301 gcaaattcgg tgaatttcaa caagaaagac ctaatgttac tgataatgtt atagattttt
     3361 caggtaaata tattgctcct ggcttagttg atactcacat tcacggtctt ttaggtgcag
     3421 atgtcatgga caatacattt gaagcaataa acacaatttc aaaaggctta ttaaaatatg
     3481 gagtaacatc tttcctacct acaactttaa ctgattctgt agatacacta agtgattctg
     3541 tagaaaatat aagcaatagc ttcaatcgag ttgagggtgc aaaaatacaa ggtatttatt
     3601 tagaaggtcc attttttaca gaaaaatata agggcgctca aaatgaaaaa tattttaaag
     3661 accctgacat caatatatta aaattatggc aaaaaaaatc gaaaaattta ataagaaaaa
     3721 tagcaattgc tccagaacgt aaaggttcaa tagaattaac aagatatgca actaaaaata
     3781 atatatcagt ttctcttggt catagttcag ctacatttga gcaagttaaa caagtcgtta
     3841 attcaggtgc taaagtgttt gtacatacat ataatgggat gagtccttta aatcatagag
     3901 aacctggaat ggttggggct gctatggtac taaaagatac ttatgctgaa ttaatttgtg
     3961 atggtcatca tgtaagccca atagctgtta aaataatgat ggatgcgaag tctagtaata
     4021 acatagcttt aattacagat tgtatgagag ctggtgctat ggaggatgga aaatatactc
     4081 ttggtaagtt taatgtaaat gtaaaaaata gtgttgcaag attaaatagt gggagtttag
     4141 ctggaagtgt attaactatg gacaaggctg tgagaaatat agttgattgg aacatatcaa
     4201 ctttagaaga tgcaataaaa atggctacat atgtccctgc tttaagttgt aatattgata
     4261 atgtctgtgg ctctatatat caaggtatgg atgcagactt tatagtaaca gataatgact
     4321 ttaatataca cgaaacttat ataaatggta tctgtaagta caaagttaat aaataggttc
     4381 aatataaggt tatggaggct gtctaaaaag agtctggaaa tatttaaagt tatttctcca
     4441 aaatctgatt taacactttt atttccatat cgttaataaa ctattgcgat agaggttatt
     4501 ttaaacaaat atatattata agtatttttg tgataatatt ttcgataata tgtacattta
     4561 aagtaacttt tctattatta tttgtaagtt tttatcttga gcaaacatat aaatttttaa
     4621 taaagcatga gataaaccct atatttcaat tactaatgat gagattttga ttgataacag
     4681 atatattttc aaatgtaaaa atactaaaaa tcatatcaat tttaaagata ttgtaggtgt
     4741 aaataaacaa gataatacta tagaaatttt tacaaatgaa agcacttatt gtatatatct
     4801 aaaagtttta agcat
//
LOCUS       DAYAOY010000179         4400 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYAOY010000179 DAYAOY010000000
VERSION     DAYAOY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.186"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            231..1310
                     /locus_tag="ACGI6B_003884"
     CDS             231..1310
                     /locus_tag="ACGI6B_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9753288.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACGI6B_003885"
     CDS             1634..2002
                     /locus_tag="ACGI6B_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFG9753289.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACGI6B_003886"
     CDS             2119..2922
                     /locus_tag="ACGI6B_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9753290.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="ACGI6B_003887"
     CDS             3314..4204
                     /locus_tag="ACGI6B_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9753291.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1678 a          482 c          828 g         1412 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata
//
LOCUS       DAYAOY010000180         4242 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYAOY010000180 DAYAOY010000000
VERSION     DAYAOY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.187"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(192..1235)
                     /locus_tag="ACGI6B_003888"
     CDS             complement(192..1235)
                     /locus_tag="ACGI6B_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFG9753292.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1256..1702)
                     /locus_tag="ACGI6B_003889"
     CDS             complement(1256..1702)
                     /locus_tag="ACGI6B_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFG9753293.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1876..3456)
                     /locus_tag="ACGI6B_003890"
     CDS             complement(1876..3456)
                     /locus_tag="ACGI6B_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFG9753294.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         1374 a          754 c          397 g         1717 t
ORIGIN      
        1 ttttacatta gtaaaggata aattgtataa aaattctgtt aatttatttt tataatttca
       61 tctttctaat aaaaacaaag aagaagactt aaaaatttat aagtcctctt cttttcaacg
      121 tgcatttttg tgctatattt aattaattcg acctttaatt tccatatcct tcctatgata
      181 acaaaactct tttatttttt taatgattca ttatcattta aaacttcttt tatatcaagt
      241 acttctttgt tatcatcact cataacttcc tttattcctt tgaatgttcc taggaaatta
      301 actgcttctg aaggtaaaac cagttttgtt gacttacctt cagccatttt ttcaagagct
      361 tccatagatt ttaatgctag aatattattg tctatatcag cctctttcat tgctgaaaaa
      421 actaatttta aaccttctgc tgttgccatt tgagttcttt ttatcatttc agcttcacct
      481 tgtgccttag caatagctgt ctgtcttata gcttcagctt caccttctgc cactaatata
      541 gcagattctt tttcaccttc tgctacacgt accatagctt cttttttggc ttctgctgtt
      601 aatatagcag attgtttttc accttcagct tgaagtattg cagctgactt attaccttct
      661 gcttgaagta ttgcctctct tctttctctt tctgctctca tttgtttttc cattgcaact
      721 tgaatatcct gaggaggcat tatatttttt aactctactc tatttacttt tataccccat
      781 ttatctgttg cttcatcaag aatagttctc atttttacat ttattatatc tcttgatgtt
      841 aatgtttcat ctaaatcaag ttcaccaatt atatttctta gtgttgtagc tgttaaattt
      901 tcaatagcag ctattggatt agctatctca aacacatatc taactgggtc tgtaacttta
      961 taatatacaa ctgtatcaat ctgcatagtt acattatctt tagttataac tggttgaggt
     1021 ggaaaatcta ttacaatttc tctaaggtca attacatatg ccattttatc taaaaatggt
     1081 attaagaaat gtacacctgt ttcagccact ttttgaaact taccaagtct cattattata
     1141 cctactttag attgttttat aactcttata caagttaaac ttattgctac gactactact
     1201 attaatacaa tacttaaaac aattttagta cccatactat aaatcctcct ttattctatt
     1261 ggtttgtcac attaactgtg gcgtctactt ttttaacaac taattttacc ccttcaattt
     1321 ttaatacttc tactgtagaa cctttttcta tcttttcacc atctatagaa atagccgtcc
     1381 atgtttcatg ttcaactaca actagacctg tgttatttgg agaaatctct tctgtaacaa
     1441 tacctttttt agacatcatt gcttgtaaat tagtgttaga tttatagcct ttatcttttt
     1501 ttactatttt tttagtagct acgactaaca ttgctataga aattactgca aatattaaaa
     1561 tttgtaagaa aatactctct ataaagctac ttaaaaatat aagtattaca gctcctacac
     1621 taaaccatat caatgttaag cttggtgtta acatttctgc tattccaaat aagactgcta
     1681 ctatcaacca tattagcttc atactatcta ccctcttttc atatgaatta taaaacccta
     1741 tataaattat atgtttatac aatgtttttg aatataaatt taaattacaa aacaataaaa
     1801 taaattaatt aattaatagt attatatcaa catactttgt taaaattgtt acaaagtagt
     1861 atcaaatatc accaattata tatccaacaa ctctttcatt gttaatgaaa tgttacttat
     1921 taaatccgta gcatttaccg tatacattgc tttgctcaat ttatctccta tatagccatg
     1981 tatatatgct ccactaactg tagcttccaa tatatccata ccttgaccta taaatgaagt
     2041 aatcattcca agcaaacaat cacccattcc accagttgcc atacagctat ttcctgtagg
     2101 atttatatat gtttttttac catctgttat gacagtttga taacctttta aaagcaccac
     2161 cagattatat ttttgagcaa attcttttgc aacatctact ctatgttctc ttatataccc
     2221 aattgaatct cctgtcaacc tggccatttc tcccaaatga ggtgtaatta caaatctatt
     2281 tttccattct aaaaacttat aacatctgtc cttcataacg ttcaatccat ctgcatctaa
     2341 tactattggg caattagaat tttctactat cttaagtaat ttatcaaatg tttgactatt
     2401 atctcccata cctggaccaa aaccaattgc atcactagaa ttaagtaatt tatttaatct
     2461 ttcttcttca aaatttaaag tcattgcttc tgtaagtcta atagatacct tttcttgcac
     2521 atcttcacta ctaattaatg ttacaagtcc actcccactc ttaacagcag attcagttgc
     2581 tatgaaactt gcaccataaa atccttttga acctgcaact atagatactt ttccaaaatc
     2641 acttttaaaa ccatgcttat ctcttcttct aatattattt tttacaaaag tcttatcagt
     2701 tatgaactct tcatcatggt attttcttgt aataaaatct ggaacaccta tcttttctac
     2761 aactacatca cctataagtg cgctagtgtc atagtttaga aaacctcttt tataaaactc
     2821 aaagctaatc gttttgtttg ctctaataca agttcctaat acatttccat tatcgctatc
     2881 tatacctgaa ggaatatcta tagaatacgt atttttgcta ttatcgttta ctatgtcaat
     2941 aacttcttta aaaataccag ttatatcacg ttttaatcca gtcccaaata tagcatctac
     3001 tacaacatca cttttagata ctctatcttt aaaaattgtt aaatttttat tttttataat
     3061 attttgtttc tcaactttaa taattttatc tacttcattt tcctctccat aatttttttc
     3121 acttaaatca tttttattaa tatttataat attaattccc atatttaaaa gtacattgta
     3181 atttatttta gagcattcac ttagtttttc aagattacct actaaaaata cgtttacttc
     3241 ttttccatat gtatttagat gtcttgctat tccaagacca tctcctccat tatttccaac
     3301 accacataca actgtataac tattatacat gtctatatcc atatttttca ttgcagatat
     3361 aaccgcattt tccatcaata caatcattgg aatccctact tcttctacac atgcagtatc
     3421 aacatttctg cttaatttag atgtacctat tagcataaat tatcactcct ataaagttaa
     3481 agacttctta aatacttacc aaaaatatct attgaaatct ttttaatccc ataaatatca
     3541 taaataaaat cactaaaact aagagttttg aaataattta aaataacaaa aaattttgaa
     3601 ttaaaacaat tatttaattt attataccat ataatttaag ttattatgtt gatttaaaac
     3661 ctatttacac taattatcta aatatctctt tatttttata atcttttata acttatttat
     3721 atttttcatt tttataaaaa aacaataatt aagaatataa ctatattcta catgtaaaaa
     3781 tttttttatt taaactcaat tttctgataa tttgataatt tttcaaaatt tatagttgac
     3841 ttttattttt tactgtatta aactaattac tatacaaaac agaaaacaaa attttcaaaa
     3901 ttattaaatt aaaaggagag gtaaatatga agactttatt aaacaaacaa gtacgatttt
     3961 ttagtagcca atttagttag ggtttgtacc taagttaaga caaattgtca taacatagat
     4021 acaagcccct taggattatg atttttaagg attgtatcta tgttggctat agtaattgtt
     4081 gttaaagttt ttattttatc atcataatat agagccacat ggattatact gtgtggctta
     4141 ttcgaatttt tgccatacag ttatatatac tgtgtggttt ttttatatca aaatcatttt
     4201 ttaaaaccaa attttaaaaa ccatttaaaa tattttcaaa gg
//
LOCUS       DAYAOY010000181         4237 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYAOY010000181 DAYAOY010000000
VERSION     DAYAOY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.188"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            74..472
                     /locus_tag="ACGI6B_003891"
     CDS             74..472
                     /locus_tag="ACGI6B_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9753295.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            460..1398
                     /locus_tag="ACGI6B_003892"
     CDS             460..1398
                     /locus_tag="ACGI6B_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFG9753296.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1782..2006)
                     /gene="secG"
                     /locus_tag="ACGI6B_003893"
     CDS             complement(1782..2006)
                     /gene="secG"
                     /locus_tag="ACGI6B_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFG9753297.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2599..3891)
                     /gene="eno"
                     /locus_tag="ACGI6B_003894"
     CDS             complement(2599..3891)
                     /gene="eno"
                     /locus_tag="ACGI6B_003894"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFG9753298.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1475 a          667 c          464 g         1631 t
ORIGIN      
        1 tatcatcaat ctatataaaa tttaataact tatataaatt taactaattt atataaaatc
       61 ttaaggagtt gccatgataa aaaaaataga acatgtggtc attactaata ataatataga
      121 aaattatatc aatttttata aaaaatttgg tttttcatct aaagaaatga gcaaaaatta
      181 taaacttaat gctagaaatg ttaagataaa tcttaacata aaaggttata aatttttacc
      241 ttatgttaaa aattcaaaag caagtagaga taacttatgt tttaaaataa acacaaatat
      301 tgatgagcca aaaaaatact tagaacaaaa tgagattcaa attaagagag gaattgtaga
      361 atgtacaggt gtctttggta atatgaaatc tatttatttg aaaaaaacta atggaaattt
      421 aattgaatta tcaaatcata gaatcacgga gagtaaacct tggaaaattt aatcctatca
      481 ataaatgttg ttcttccttt gtttctcaca atgtcacttg gatatgttct aaaaaaatta
      541 ggtctatttg atactcatac tttaaataaa atgaataatg tttgcttcaa gtctttcttg
      601 ccattattat tattttttaa tatatacaat acagatttag atggttctct taattttaag
      661 cttatggctg tagctgttat aattattttt tcaatcttct taatcttagt ctttattatt
      721 cctaaaattg aaaaagataa taagacaagg ggtgcattaa tacaaggtgt atttcgtagc
      781 aatttcgtta tatttggtat tcctgtgaca atttcactat ttggcgataa gagtattgga
      841 gtcacatctt tattaattgc agttattgta cctatgttta atgtattatc tgttgtaatt
      901 cttgaaattt ttagaggtgg caaagtaaat ataaaacata tgattaaaag cgtcttaaca
      961 aatccactca taatcgcctc ttttatagga ttgacaatgc taattttaaa tattaatata
     1021 cctacatttt tacacaaaac tattggtgat atttctaaaa tagctactcc tttatcactt
     1081 ataattctag gcggctcttt tagtttttct gctataaagg ggcatataaa acccattata
     1141 ttgggcgtat gtggaaaact tatacttgta cctgctatat ttttaccaat tagcattttt
     1201 cttggattta gaaatgtaga attagcttca cttatgataa tgttatctgc tccaactgca
     1261 gtttcttcat ttacaatggc acaacagatg gattctgaca gtgaattagc tggacaaatt
     1321 gtagtattta cttctggatt ttctataatt acagtatttt taataatatt tattttaaag
     1381 cagaataatc ttatttaatg atatttgata acacaaattg gtattataag aaacatatat
     1441 aaaactgatt taatcgcgta aaaacatatt attatactat ttttactata taatataact
     1501 ttgtgtatat tatctcatta aacatggtga aagtagtgtt gctatatatt ttttagtttg
     1561 aagaaaatat ataaatacaa acaatatata aatacaaata ataaacgcca ccaaatagtc
     1621 aatctataaa ataaaaactg gactaatcat ataaaatcat tagtccagct tttattttac
     1681 gtacttttta tgttgatgta cttttattaa aaactaaatt taatttttaa atgtctagca
     1741 aatgcactaa ttaaataatg gccagttatt caacaatatc actacttctt aactataagt
     1801 aatgccaatg catttataaa aaataatact gctgttatct ttgtaactct agcaagaaat
     1861 gcttgttttc cttttggttt aaagtaagct tgacttcctt ctccaccaaa ttctgaaggt
     1921 aaagcattct tagaatcttg aggcattatg ctaattacta gtaaaatccc tagtactact
     1981 tgcacaccca ttaaaatgtt gctcattctt gcccctccta tctgtttatt acttaaattt
     2041 cattactctt tagtgtatca caataatata gccatttcaa gactattatg ctataaataa
     2101 catacaaaaa aatatattgt tactataata tattactata ttctcaatat aaaaatgaat
     2161 aaaacttaga agagaaaact atttaaatta atacataaat taacacttaa aataatctac
     2221 tctcattgta tatattataa atagccacca aaataaaaca atcattttaa aatattgata
     2281 taacttttaa tttcaacata atttaaaata aataatccac atttagcaat aatacttcaa
     2341 tgacaattat gattaaatca aaatatgtct tcacttttat tttttaataa aaacctccta
     2401 tgcaattaga taaaaatgag atagaaacat ttattcatat tttttctgaa tctatgaata
     2461 aaattaagta ataaaatata aaaaaggata agtgaattga aaaatcaatt aacacttatc
     2521 tcttttatat cttataagat gagcttaaat atcttatatt ctctcttcat taatcttttt
     2581 tagtaaccat tttaataact atttttttaa gttatagaaa gattttaatc cacaatatct
     2641 agcttgctct ccaaccattt cttctattct taataattgg ttgtacttag caactctatc
     2701 agttcttgat ggagcaccag tttttatttg accagcattt actgcaacag ctaaatctgc
     2761 tatagtagaa tcttcagttt ctcctgatct gtgagatata acagcagtat atcctgctct
     2821 cttagccatt tctatagcat ctaaagtttc agttattgta cctatttgat taactttaac
     2881 taatatagag ttagctactc cattttcaat tcctttttct aatctctctg tgttagtaac
     2941 aaataagtcg tcaccaacta attgtaattt atttcctaat ttttcagtta ataatttcca
     3001 tccatcccaa tcttcttcgt ctaaaccatc ttctatagtt attattggga agttgtttga
     3061 ccaatcttca tataaagcaa ccatttcagc agcagttaac tcttttcctt ctcctgctaa
     3121 aacgtatttt ttagtttctt tgttgtacat ttctgtagca gcaacgtcaa gacctaacat
     3181 aacgtcttca cctggcttat atccagcttt tgttatagct tcaactatta attctaaagc
     3241 ttctctgttt gaacctaagt ttggagcaaa tccaccttcg tcacctacac cacaagctaa
     3301 tcctttttct cctaaaactt tctttaacga atggaatact tctgctccca ttcttaaacc
     3361 ttctttgaaa caacaagctc ctactggtaa tatcataaat tcttgaacat caacgttgtt
     3421 atcagcatgc tctccaccgt ttaatatgtt catcattgga acaggtaatt gtttagcatt
     3481 tactccacct aggtattgga ataatggtaa acctatttca tcagcagctg ctctagctac
     3541 tgccattgaa acacctaata tagcattagc tcctaattta cctttgtttg gagttccatc
     3601 taattctatc atgattgcat ctatagctgg ttggtcaaat gcatccattc cttctatttc
     3661 aggtgctatt atttcattta cgttagcaac agctgtttct acaccttttc ctaagtatct
     3721 tcccttatct ccatctctta attcaacagc ttcaaaagct cctgtagatg ctcctgatgg
     3781 aactattgct cttcccattg ctccatcttc taatactact tcaacttcta cagttggatt
     3841 tcctcttgag tctaatactt ctctagcata tactaattca ataactgaca tttgtatatc
     3901 tcccttcgat tttctattag ttaaattttc atttactgta aacatccgtc tactatttta
     3961 atctactttc tatattatag tacataaagt tgcttttgaa aattaggtac aaatttattt
     4021 gatgggtaaa tttgtaaaag ttcctatttt tattttattt tttataatat tttaatttat
     4081 ataatataaa aaaacttgtc taaaatccaa aatcaatctt aaatacacaa atattataac
     4141 tagatttaaa acattaaaca taaaatgtac aactaatgat tctaaaattt ttgcttatat
     4201 aatatatatt aattattatt atttatattg ttattgt
//
LOCUS       DAYAOY010000182         4021 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYAOY010000182 DAYAOY010000000
VERSION     DAYAOY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.189"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(268..648)
                     /locus_tag="ACGI6B_003895"
     CDS             complement(268..648)
                     /locus_tag="ACGI6B_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753299.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(849..2042)
                     /locus_tag="ACGI6B_003896"
     CDS             complement(849..2042)
                     /locus_tag="ACGI6B_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9753300.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2105..3325)
                     /locus_tag="ACGI6B_003897"
     CDS             complement(2105..3325)
                     /locus_tag="ACGI6B_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9753301.1"
                     /translation="MIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLIDTIFGTVLLKVT
                     DIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGEEEIIPLRLVPFI
                     ISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLVTGMAAGFAGLFE
                     TPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFSFALSSKVVFDLS
                     IFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGVCLSVLFLLFYKG
                     RYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTPLFSIGASLGVLL
                     AGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYFFVVCAISYIFNM
                     DKSIYSLQKISTKQ"
     regulatory      complement(3626..3702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1463 a          737 c          417 g         1404 t
ORIGIN      
        1 aatgaacgaa tattatatat atcattcata aaaactttat ataaagaata gaaacaagtt
       61 ctagattact atagagatga catatcaaaa tatttttaaa aagtaattta agtattagaa
      121 gtaattgaag ttagtaaata taaatgcgtt tacttttaag tattgcttaa gggaataaaa
      181 aataaaaata aactctgcaa ccatctaaaa atagttgcag agcttacaaa accaaaatta
      241 tctaaattgt aataaaaata ataacattta tacataagct tcttcccaat ttactaccca
      301 gttttctaac gactttatct tttcctctgt tagctcctct tcgtgaaatg atactaataa
      361 attccttata aaattattct tcaaaagatt ttgcacattt cttgatataa actctaaata
      421 tgccttttct tttattgcaa ctgtataata catacacttg cctgtttctt gaacatataa
      481 gaatcttttc tttgatagtt ttgataaagt ttttaaagct actttttctg gtaaattatg
      541 ttcttctttc atataatcaa taatctcata tgaattggtt tttatatcta aattccaaat
      601 aaatttcata ataactaact ctaattttga taattttttt aaacacatag ttatcactcc
      661 gcttctttat ctatataatt aaatattaca ataataattt cacaaaatca attctaataa
      721 tcataacata ccttaaaatc tgttcttaac ttctaccttc ttaatctccc aaatgatttt
      781 tttatattgt attcaatagt ataatccttt tccaaaatat aacttaccag ttaaaataat
      841 ctttattttt acttactctc gccttttaca gaagttaata attttttata agttacatca
      901 attccaaaag ctccaagagg tgcagttata agtatagcta gaactgctaa cgtcaaaata
      961 agttgtccag atgccatccc cattgcaagt ggtacacctc ctattgctgc ctgtactgta
     1021 gcctttggaa tatagccaat catacagaac aacctttcct ttattgtcaa atttgtctta
     1081 ataaggcaga agaatactcc taccatacgg aataaaagaa ctccaaatat taaaattaca
     1141 gctccaattc ctgctttaaa tgcataactt atatctactg ttgcccctac caaaacaaac
     1201 aagagaattt cagcgccaac ccatagtttt gaatacttta tggacaatct tcttgcaact
     1261 acacgatatt ttatctgaag gaaaatccca atactcataa ctgctaataa tccagaaaat
     1321 ggcacaatac cctctaaagc agtctctaaa cttatcatta aaaatgagat acttaaaagc
     1381 aacacccctt tagcagaatc tctcatatga accttcttaa aaaatactgc taaacagatt
     1441 ccaatcactg ctccaactat tacacctaat ataattgata ctggaatttg taaaaaacta
     1501 atagctgata tactatttcc ttgtgccaat cctgtgaata ctgtaaacat cacaattacg
     1561 aaaatatcgt ctatagaagt tcctgctaaa agcatctgtg gaatactttt ttctgtacca
     1621 taaccttctt ccattaattt aagcatttta ggtacaataa ttgcgggaga aactgctcct
     1681 actactgcac ccatgactgc tgcttccaat atagaaacgc ctagtaattt tggtgctaaa
     1741 actatcattc cgataatttc aaaagtagct ggaataaagc acataagtac agcaggtctt
     1801 cccactttct tcaaatcgtt tatatctaaa gataatccag cccttgttaa tataataatc
     1861 aatgcaatct ttctaaggtc agaagaaata tctagtattg aattatcaat caaatttaaa
     1921 acatatggac ctaatataat tcctgtaagt aacattccca ataagttagg taactttatc
     1981 ctcttaaaaa tacctcccga ggccattcct aataaaaaaa tcaaagctaa acttgtcaac
     2041 atattcctac cctatccttt ctctaatatt ctttctcttc taatcataca ttatgttgtt
     2101 ttatttattg ttttgttgaa attttttgta gtgaataaat agatttatcc atattaaata
     2161 tatatgaaat agcgcatact acaaagaaat aaggtaaata gctgtatcca aaaacttctg
     2221 cccctataaa tacaggtgca aaaaaagtat ttgtagcact accaaagaca ctagcatagc
     2281 caagcgctgc aactaattca attggcaaat taaaaaatcc tgctaacaat acaccaagac
     2341 tagctccaat ggaaaacaaa ggtgtaacct ctcctccttg aaaacctgct gacaaagtca
     2401 gtattgtaag tataaacttt aacagccaat caaatgaata aatttctcca ccatagaaac
     2461 tattttgaat taaatttgtc cctaaacctg aatatcttcc tttataaaat aaaaggaata
     2521 atacactaag acatactcca ataatagcaa ttcttatcat aggatttttt aatctattac
     2581 ctatttttct ttttgacaat ttcagacacc atgcaaatgc tccaccaacc attccaaaaa
     2641 taattcctaa aacaataagc ttccaaaata ttgacaagtc aaatacaact ttagaagaaa
     2701 gtgcaaaaga aaatttctct aaccctaaag cttttgatac tgaacttgct gtaaaagaag
     2761 cagtaaacgc aggaaaaagc gattgatact ccagtgaacc agctaccaaa acctccatag
     2821 caaatagtat tgctgctatt ggtgtctcaa ataatcctgc aaaaccagct gccataccag
     2881 ttactagaaa aatactacaa gcatttttta taggcaacct tttacctacc caatgtgaaa
     2941 aagttgcacc aatctgaaca gcaactcctt ctcgtcctgc actaccacca aataaatggg
     3001 ttaaccatgt acctgatatt ataaatggaa ccaatcttaa aggaataatt tcttcttctc
     3061 catgccctac ctcaaatatt agattcattc ctttactact ttttcctcca aatttaaaat
     3121 agcaataagc aataacaact cctactactg ctaaaaatgg tatcagatac attggatatg
     3181 tttctcgaat atctgtaact tttagaagaa ccgttccaaa tatggtgtct ataagaccta
     3241 taattgcacc aactggtata cctatgagtc ctaaaaaaaa tagtccacca tatgttttat
     3301 aaacgctttt aattcttatt atcattttaa tccatattaa agttttaaat ttttctatca
     3361 ttttaattcc tttctatttg catcataaga ttttttgaaa gttttattac atcaactgta
     3421 gtcaatgtaa tgactatcat aattgttttt taaattgata ggatataggt acactcttaa
     3481 cccattctta ataaaataat taacatataa tctactattt tttctattaa ttaaatattt
     3541 cttcaaattt aaaaaataaa aatttagtaa gtacttcacc tccattttat tgatttaaac
     3601 aacaaaaaaa gctggtggca tctatgatat catagaagtc atcagctcat tggcagttta
     3661 ggatattatc atccaaggga gaacctcatt cccataatta ttatataatt ttttattttt
     3721 catatgtcta ttttatgtaa attgtatttt taatgttata aataatataa cctatcaatt
     3781 aaaatagtaa agcctctaat attatgagtg ttcaatattt catattattt ttatgatata
     3841 taatttaaat gattttcttt actttgcatt atgatacata acttaaatat ttttcctcac
     3901 tccacattat gatatattat ttaaatattt ttcttcactc tgcttatatt atgatacatg
     3961 atttaaatat tttcttcact ctgcttatat tatgatacat gatttaaata ttttcttcac
     4021 t
//
LOCUS       DAYAOY010000183         3917 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYAOY010000183 DAYAOY010000000
VERSION     DAYAOY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.190"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..1396)
                     /locus_tag="ACGI6B_003898"
     CDS             complement(<1..1396)
                     /locus_tag="ACGI6B_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9753302.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGF"
     gene            1537..2433
                     /locus_tag="ACGI6B_003899"
     CDS             1537..2433
                     /locus_tag="ACGI6B_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9753303.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            3098..3238
                     /locus_tag="ACGI6B_003900"
     CDS             3098..3238
                     /locus_tag="ACGI6B_003900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753304.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(3235..3606)
                     /locus_tag="ACGI6B_003901"
     CDS             complement(3235..3606)
                     /locus_tag="ACGI6B_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9753305.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT         1577 a          439 c          515 g         1386 t
ORIGIN      
        1 aaaagccaat taatattgca cctattaagc aaacaagtgt aacaataata taaacatttc
       61 tcattccaaa tacaaatatt ttatcattat taatcacata atcattgacc ctatatccag
      121 ctttaatact cataaaatta tataaaagtg ttgtagaaat agtaatacct ataacttgtc
      181 ctaaatttct tacaagggaa tttatgcttc ctacaactcc tagcttactt ctagaacagc
      241 ttgacattat caaagcattg ttagcaggtt gaaatatagc ttgtcctatt gatattatcg
      301 atatataaat tacaaataaa ataagggcag aactttcttt caatcttgac attagaaaga
      361 atccaaaact cataacgaga agaccaatta aaataatttt ttctgactta attttatctg
      421 atatattccc aaatataggt gaaaaaatgg ctaaaatcaa aggtgatagt atcataaata
      481 aacctgcttg aataggggga agtttcatag tactttgtaa ataaaatgga attaaaatac
      541 ttgatgatgc aatacaaatg aatgatgtaa gagcacaaat taaattcaat gaaaatttaa
      601 aatttctaaa aatttcaagg tcaagaagcg gttgaggatg ttttttttct aatatcaaaa
      661 ataatataat aaaaataatt gataataata gtgcaattaa tatatatgga tttagtaatc
      721 ctgtttgttg agcactaatt agagcactaa aaaataatgt tgttcctaaa aattgaagta
      781 tagcaccagt tttatccata ttttctgaag attttttact attaggaaga aattttgtat
      841 ttaaaaagat tacaataaga ccgattggaa tatttataaa aaatataaca ttccaatcaa
      901 atattgacaa aataaatcct cccacagatg gtccaaccat atttccaata gcaactgcag
      961 taactaaaat ccctagggct tttcctctgc tttcttttgg aaatagttct gtgataattc
     1021 catgattgtt tgccatgtag gcagaagcgc ctattccttg aataaagcgg caaactataa
     1081 gtaaagttaa agaattactt aatccacata gtaatgaagc tgatgtaaag agaatagtac
     1141 ctacttgaaa tactcttgat ttaccaataa tatctccaag tcttccaaaa aataatagtg
     1201 ttgaacaaat aataattgag taacttgcta ttacccattc aatagatgct agagatacat
     1261 ttagtttgct tgcaagaact gggagagcaa catttataat actagaatct aatgttgcca
     1321 taaaagtcat ggcaatgact acaaataaaa ctgtgatttt ttttctatta tcatcttttt
     1381 taaaagagtt tgtcattttt attacctcct tattataatg actgcattat gttaatgttt
     1441 acaatgatag ttacatttta tataatagcg atatgaaata atgaatacaa taactatatt
     1501 gcttataatt agcactattt tgatgagggg ggaataatga aggataaaaa tacatatttt
     1561 aacaggaaag gtcaagaaaa ctttatagat atttttttaa aggatgacat taaaaaagga
     1621 gcaaacttta aaagacattg gcatgagcat ctacaaatat attattttat tgatggtata
     1681 gcatatcttg aatgtggatt ggataatttt aaggtagtgc ctggaaatat tgtaatagca
     1741 aacagtaatg aattacatta tttagaaagc ttatcagata atttaaaatt ctatagtatt
     1801 cgtattgaac ccacattcct ttttagtaat caagttgatt tgttacaaac caagtactta
     1861 gcaccattgg ctttaaatca tattgtattt aataatttaa ttcaaaatga ttctctgatt
     1921 ttaaaatgta ttacaactat acttgatgaa tatcacaata aagaagttgg atatgagtta
     1981 tctataaaag ctaatatcta tcaattaata gtactcttat taagaagtca tattgataaa
     2041 atatattctg aagatgaatt tataaaaaga aagtattctt tagaaaaatt tgaatgtatc
     2101 tttgatttga tagaagaaaa atatatggat aaaattacat taaaagaact tgctagtcat
     2161 gtgaacttga gtgcacatca cttttgtaga acatttaagc aaataacagg taagactaca
     2221 atcgatttta taaatggaat caggcttgat aaagctatat attatttaaa ggaaacagat
     2281 ttaaatatga cagaaattgc tatgagatgt ggctttgata gcataaatta ttttagtcgc
     2341 ttatttaaaa ggaattataa tatatctcca actaagtata gaaatgaaca taaagaagtt
     2401 agtaatgtta ttttaaaaga agatatgaaa taattcataa atatacttta tagaaaatat
     2461 atatttttaa atctttgagt ttttcttaat aaatattaat aaagtatagt acataaatga
     2521 gtaagactac ttgtatagtt aaatgtgaaa atgagtatga aagaatgtag tgtctatgaa
     2581 aagtatattt taatatgaaa ctaaaattgg aaaaataaga atagcagaaa aaatgattat
     2641 attataaatc agtttttttg agacatctgg cttagattct gaaagatatt taatcaaaga
     2701 aataaaacta ttaaataagc aagtaaacag ctaaaatagt atgattttta tagaaaattt
     2761 aaatccatcc taaagaatta aaagaaagtc aaataaatat aaaaaaatta tatatttata
     2821 aaaaatttta taataaaaat catcctgaaa aatatataaa aatagaaaaa acatacaaga
     2881 ttctacaata atagattttt ttgttattat tacgagtttt ttattgcaac tgtagaataa
     2941 aagtattatt atataattaa gataattatc tagggaaatt ttggataatc aaaatatatt
     3001 ttataaaatc aaaatacaat tacataaact ttattaaaaa tttgttggga atttctgact
     3061 aacattaaga acgtaagttt gttgggggat ggtatatgtg gaaaagtcaa acactactgt
     3121 tacaaaaaaa gaaaaaattg aagatatagg aatgaaatta gaacatttat tgagtgaaga
     3181 tattactttt tatgaattat tacttgagat tttgtataaa gaaaaagaag agaattaatt
     3241 ctcttcttta aggaatcttt ttaaataggc attatcaatc attttttcta tagcttctct
     3301 atcttctttt tcaagagaat atattttttc taagagcttt tttgtttttt cacttactga
     3361 taagttctct atgattaatt tatcttcttt gcttattttt gtattatcat ttacgcctaa
     3421 taaataatct gttgatgttt ctaatgcttt tgctaatcta actattattt cacttttagg
     3481 ttctcttaaa ttattttcat aacgtgacaa gctagcctca gttataccga ctttagtagc
     3541 aagttctttt tgatttatat ctaattcttt tcgtagattt gctattctat ctcctaaact
     3601 ttccatatgt acatcactcc ttaccgaaat tataagttat tttgataaaa agtaaaataa
     3661 aattaccaaa aatataaaaa aaaaaggtta aaataataaa atatattgaa aattaccatt
     3721 tgtatatgta taatatgaat tatacaaatg gtaatttgat gagaggtggt atgttgaaat
     3781 aagaaactga attaattaat aaatacatag aaagttttga aaacctttaa ttgagatata
     3841 aacaatatgt acaaaatttt ttgcaagtaa aattaccaaa tgtataaaat aaatcagtag
     3901 catagataat tttatgt
//
LOCUS       DAYAOY010000184         3866 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYAOY010000184 DAYAOY010000000
VERSION     DAYAOY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.191"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            202..1476
                     /locus_tag="ACGI6B_003902"
     CDS             202..1476
                     /locus_tag="ACGI6B_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9753306.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1683..2009)
                     /gene="trxA"
                     /locus_tag="ACGI6B_003903"
     CDS             complement(1683..2009)
                     /gene="trxA"
                     /locus_tag="ACGI6B_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFG9753307.1"
                     /translation="MYIMVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYE
                     ALGNEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKN
                     KIESYM"
     gene            2267..3019
                     /locus_tag="ACGI6B_003904"
     CDS             2267..3019
                     /locus_tag="ACGI6B_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFG9753308.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3006..>3866
                     /locus_tag="ACGI6B_003905"
     CDS             3006..>3866
                     /locus_tag="ACGI6B_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFG9753309.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVL"
BASE COUNT         1512 a          511 c          546 g         1297 t
ORIGIN      
        1 tcaacctatc tcccccaaaa tcaagtgagc tacagtttcc cccaactgta gctcattttc
       61 gtgtaattta ataaacatct caacaagaat taattaacaa aaccccttat aaaaacataa
      121 catatattaa acaagctctt gattaaaact tgagcttttt taataaaaaa cagaatagtt
      181 tattagataa ggggtttttt tatgcaattc atggatgtat ctaaatacat tgaaggtgca
      241 aaccttaaag ttaaaactaa aagtggtgaa actgttaaaa gaatatattt taacaactct
      301 gccactccat tagttttaaa aaacgtagtg gataacttaa actgtgagat tcctttgctt
      361 acttatatta atgctccagg aataatctca gaaaaaaata ctttaaaata tgaaaatgta
      421 cgctctacta tcctcaaact tattgatgga gacgaagaaa aggattctgt tatttatgtc
      481 aaaaatgcta cagaaggaat aaacttctta gcaaaatttt ttaaagatga aaatccagat
      541 aaatttgtta ttaccacagc aatggagcat atggctaatt acctgccttt taaagttaat
      601 tttcctacaa aagttgttgg aattacagaa aaaggagaac ttgatttatg tcaattagag
      661 aatatactta aaaattatgc tggtaatgta tctctcgtta cagttacagg agctagtaat
      721 gtcacaggta taaccactcc tatatatgaa atagcaagaa tagctcataa gtatggtgca
      781 aagatattgg tggatattgt gcaagtaatt cagcataaac ctttttcaat gatgccacat
      841 gaatgtgatg agcatataga ttttgttgtt tttagtgctc ataaatgtta ttctccacta
      901 aatggaggag ctctggtagg acctaaaaaa ttctttgaaa aatttaatcc tgtactatat
      961 ggctcaggtt ctaccaaatt tgtaaatgat aacaaaatac tatatgcaaa cgtaccccaa
     1021 cgttttgaag caggataccc agagtatttt ggaactcttg caatgggaaa agcactgaac
     1081 actttaaata aaattggtct aaacagaatt tcaaaatacg aaagagaatt atttttatat
     1141 gctaaagaag aaatgagtaa aatacctaat ataataatat atggagataa atcaaataat
     1201 gtaattatcc caaatatatc ttttaatata agtaatatgt attacaaaga tgtagcaaaa
     1261 tatttagttg ataactttgg aatagaaact ggagctggag tagtaggtgc agatatttat
     1321 gtacaaaaat tattaggtat aagtccaaaa gaagcatatg tcagatttat gagggaaaat
     1381 cctgtaggtc ttgttaggat aagcttagga atgtacaata cttttgatga ggtggatagc
     1441 tttatacatg ctattaaaac tttggctttt aaataagttt tttctattct tttttatcaa
     1501 taaatagaga gtagttttgt tttcacaaaa aagcaaatta tatcaacagt caatttcatt
     1561 agcaattaga cactcatttg taactataca aactatttta cactatacaa acaaaaaact
     1621 cattacaatg agtttttttg tttatatatg agatttattt taattacttc aatttacttt
     1681 aattacatat atgattcaat tttgtttttt aaattctcct taggcataaa acctattaat
     1741 ctatctacag gttttccatc cttaaatatt agcatagttg gtactgtaga tacttcaaat
     1801 ttctgagcta attctatact ttggtctatg tccaccttca agaagtttgc tttttcaacc
     1861 atctcatttc ctaaagcttc atatattggt gatagcatct tacaaggtcc acaccaagtc
     1921 gcaaagaaat ctacaactac taaaccatct ttattttcta cttcatttat aaattcatta
     1981 ttatttacta tttttaccat aatatacatc tcctttatta tcttcttgat attaatatac
     2041 tacgacctat ataacagttc aattaggcac ttttttgtaa ccgcctattt ttaatagatt
     2101 taatattatg ataaatagat ttataagatt aaatatttat aagactaaat atttctaata
     2161 tattttattt ttttcttaat atagttgaca ttttttccct taaattataa aatcaaatta
     2221 ataactacat aggtagttac taatctttta tcaaaggagt ttctatatgg ataatattat
     2281 taatgaatta caaaatatag gatttactaa atacgaatct caaatataca tatcattatt
     2341 aaaggaaagc cctttaacag gatatgaaat aagtaaatta tcaggtgtac cacaatcaaa
     2401 agtctatgaa aatataacaa agctattaaa caataatata attatcaatg ttggaactga
     2461 ccctattaag tacataccaa taaaccctaa tgaattatta aaaaataaga aaaaagaatt
     2521 tgacaattct atggataatc taaagaatag cttacagata ttaaataaaa gtaaaagtgt
     2581 agaatacgta ttaaacataa aaggagataa aaatatatta aaaaaagcaa tagaaatgat
     2641 aaaaaatgca aaacaagaaa ttataatttc ttgcccttat gaagaaattc ttgaattaaa
     2701 aaaagaactc ctaattgctt acaaaaaaaa tattaatatt gaaatactac ttttagaaaa
     2761 caaaagtctg aaaggtcttg ataatattca tattcatgga ggtaataaaa ctacacttga
     2821 aaataaaatg ctttacaaag gaattatttt gattgtagat aatgatgaaa tattgacagg
     2881 aaacttatca gaaggcagtg aggccatttc aatttggaca aaaaactcta acattttata
     2941 tattggagtt caatacataa aacatgaaat ttatatttca gaacaaataa aaggaggtta
     3001 taaaaatgaa caaaattaag attaaacaag ttgatgcttt tacaactata ccttttggtg
     3061 gaaatcctgc tggtgtcgtt actgatacct ctaacatttc tgatgaaatt aagcagaatg
     3121 tggctaaaga aatgaattta tcagaaactg catttgtttc agagtcaaat attgcagatt
     3181 ttaaagtaca atttttcaca ccaggtttag aagttgattt atgtggtcat gcaacaatag
     3241 gtactttcca tgcacttttg gaagaaggga agcttaatac taataaaagt atattttatc
     3301 aagaaacaaa agctggtgta ttatctgtag agcttaagaa aataaataat gaaaatatat
     3361 ttatgatgga acagaataca cctgaatttg aaaatttaga taattgcaga aaagaacttg
     3421 cacaaatgat aggtgtgcat gaagatagac ttcttgaata tcctattata aaggtaaata
     3481 ctggactttg gtggcttgta tttggtctaa aatctttaga tgatttaaaa aatatctctc
     3541 cagatttaaa taaaatcaaa aaatttagtg gtgataataa tataattggt ataactccat
     3601 tttgtataga aactatagat aaaaagtgtg actaccattt aagatctata gcaccatatg
     3661 ttggagttac agaagaccct gtatgtggaa ctgggaacgc ttgcgtatca tcttatatag
     3721 tacatcacaa tattttagat aaaactagtt ttattggtga gcaaggtaac tttataaaca
     3781 gacctggaaa ggtatatgta aatataacta gagaaaacaa taacataact tctgtaaaaa
     3841 ttggaggaag tgcttatact gtctta
//
LOCUS       DAYAOY010000185         3816 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYAOY010000185 DAYAOY010000000
VERSION     DAYAOY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.192"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..231)
                     /locus_tag="ACGI6B_003906"
     CDS             complement(<1..231)
                     /locus_tag="ACGI6B_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753310.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIP"
     gene            443..2266
                     /gene="typA"
                     /locus_tag="ACGI6B_003907"
     CDS             443..2266
                     /gene="typA"
                     /locus_tag="ACGI6B_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFG9753311.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2356..3264)
                     /gene="trxB"
                     /locus_tag="ACGI6B_003908"
     CDS             complement(2356..3264)
                     /gene="trxB"
                     /locus_tag="ACGI6B_003908"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFG9753312.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
BASE COUNT         1321 a          604 c          517 g         1374 t
ORIGIN      
        1 tggtatctgt tgttttctca ctatttccaa ttccttaatt tctaaagact taatcacatt
       61 tactttatag ttttctttag actttatata caaaacatct tctatctttt taccttcaat
      121 aaatttatga gaccacttaa gaagttcttc aaaattacaa acttcttctt ttacaaaagt
      181 atcactttca cttgcaaaat atttaattat tattttggtt acttccaaca tatatcatcc
      241 tttactcttt tattaatctt tttatctatt aaggttatta tttgtctaaa aaattttttt
      301 aaacaaaata attttcagat gcaaaactaa cagttttatt caatttaaac tatattaata
      361 caatttaata tattcctcct tgtacctaat aatatattgt gctataatta ataagttatt
      421 ttaatcgaag ggagcaacaa aaatgtcaca aaaacataaa attataaata tcgcagttat
      481 agcccacgtt gacgctggaa aatcaacatt ggttgatgct ttcctttctc aaagcggtgt
      541 ttttagaaag aacgaagtag ttaaagactg cgttatggac agtaacgacc ttgaaaaaga
      601 aagaggcata actatatatt caaaaaactg tgcaataaac tatgaagatt acaaaataaa
      661 catagttgat actccaggac acagtgactt ttcttctgaa gttgaacgtg ttatgaaaac
      721 tgttgacaca gttattctat tagttgatgc tagtgaaggc ccaatgcctc aaactagatt
      781 cgttttacaa aaatctttag agttcggttt aaaaccaata ttatttataa acaagattga
      841 taaaaaagac cagagagcgg aagaagtagt taatgaagtt ttcgatttat ttgtagactt
      901 aaatgcaaca gatgagcaat gcgaatttcc tataatatat ggtattgcta aacaaggtat
      961 tgctaaactt gaaatggatg atgatagtga agacctttct cctctattta aaactattgt
     1021 aaatcacgtt gaagcttatc ctaattacga taatgaacct ctacagttcc aaatatcagc
     1081 tcttgcatat gatgattatg taggtagact tggtataggt agaatttaca aaggtactct
     1141 taaaaacaat acacaagttg ctatatgtag agaagattct gttgtatcta aaggaaaagt
     1201 atcaaaactt tctgtgtatg aaggtttaaa acaagttgaa gttgatgaag ctactagtgg
     1261 agaaatagtt gttatagctg gtattcctga tatatctata ggtgaaacta tatgcgattt
     1321 agatagtcca ctacctatgg aaatgataaa aatagaagag cctactcttt ctatgaactt
     1381 cttagtaaac gactctccat tcgttggaaa aagtggaaaa ttcgttacaa ctagacattt
     1441 gaaagataga cttgaaaagg aacttgaagt aaatgttggt cttaaagttg aacctcttga
     1501 tactactgat ggatacaaag tttctggacg tggagagctt cacttatcta tactacttga
     1561 aaatatgaga cgtgaaggtt atgaagttgg tgtttctaaa cctgaagttt taatgcataa
     1621 agaagatgga aaattaatgg aaccaataga aagagttgtt gtaaactgtc ctgaagttta
     1681 ctctggtact attataaatg aattaaacat gagaaaaggt atgatggagt ctatgtcaat
     1741 agaaggagac tatgtaaaaa tagagttctt agcacctact cgtggtcttt taggatatcg
     1801 aagtgaattt ataaacgcta ctcgtggaga aggtacttta gttcgttcat ttgaaaaatt
     1861 tgaagaattt aaaggagaaa taccttcaag aggaaatggt gttttaatag cacaaggacc
     1921 tggtgttact atggggtatt ctcttaatgc cttaagtgat agagctgtaa tgtttgtaga
     1981 ccctggtgta gaagtttacg aaggtatgat aattggtatg aattccagaa aagatgatat
     2041 ggtagtcaac ccttgtaaaa acaagaaaat gagtaacgta cgtgcttctg gttcagatga
     2101 tgctataaaa ttatctcctc ctagaatatt tactcttgaa gaagcactag agtttataga
     2161 agatgatgaa ttagttgaaa ttacacctga ttctataaga cttagaaaaa gattcttaaa
     2221 tgaacatgat agattaagat ataacaaatc tagacaaggt aaataaaacg tacatttacc
     2281 tctaattaac aaaatagcca tatagtactg aaaaattatt tcagtttata tggctatttt
     2341 tatttgaaat ttatattatt caacatttat ataattagct gcttgaacgg ctgcaatagc
     2401 tccatctgat gctgctgtta ttatttgacg taaaaactta gttctactat ctcctgctac
     2461 atatacacct tctactgatg ttatacaaga ctcatctgaa attatatatc caccttcatc
     2521 caaatcaaga acattttcaa acattttatt attaggttta agacctatag caataaaaat
     2581 accactaaca tctatagttc ttttttcttc agtctttaag ttttttactt ctattttcga
     2641 tacagtcttt tctccttcta ttttttctac accatgactg taaagtatct caatattctc
     2701 tcttgctttc acagatttct ccaacacttc ttcacctcta aaactatctc ttctgtgaat
     2761 taaatacact tttgtacaat tattagctaa aaataaagca tcttctagtg cagtatttcc
     2821 tccacctact atagcaactt ctttatcttt aaaaaaggct ccatcacatg ttgcacagta
     2881 agatactcct ctacctgtaa attcttgttc ccctgcacaa cctaatttac gtctttcaac
     2941 tccatttgca agaataacag cctttgcttt atgttcacct gaagaagttt ttacaacctt
     3001 tacttttccc tctaaattta tctcttcaac ctcatcaaac tgaatatcaa caccttgagc
     3061 aactgcttga ttgtatatat tattagaaaa ttctacccca gaaatctttt gaactgctgg
     3121 ataattttct acctcagagg tacttgcaac ttgacctcca taaatatttt tttcaaatac
     3181 tgtaacgctt aaccctgctc tcatagcata aattgctgaa gttaaacctg ctggtccagc
     3241 accaattaca atgatatcta ccataacgtt tacctctctt tatattatta tttcttatat
     3301 attattctca tcattcttat actttactat tattatccat atatactata aacaaaacat
     3361 tattttttat taattaactt tattttacat atatataaaa aaactgcaaa taaaaattaa
     3421 cttttattta cagttctcac ttatttatat aattcaaaac cttattggtt atcatttaca
     3481 gtgtcttcta atatattgct ttctgatata tatgatagaa ttgaaagtcc atacataacc
     3541 aaaactaatg ctgctgttat aagtactatt cccatattta atacctcctt ttgtttacag
     3601 tatactatta ttatgtgaaa tatttatgaa tttaatttga atatatatgt aatttcacac
     3661 taacaatttc ttcaaattaa acattattaa ctatatactt ttgttgaacc aattatgatt
     3721 aatagtttaa atattagcac tatttactta tatttatatg atataccata taattacatt
     3781 tttcaaatta aagttttgta aacttttttt gtaata
//
LOCUS       DAYAOY010000186         3772 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYAOY010000186 DAYAOY010000000
VERSION     DAYAOY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.193"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            254..1126
                     /locus_tag="ACGI6B_003909"
     CDS             254..1126
                     /locus_tag="ACGI6B_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753313.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            1123..2412
                     /locus_tag="ACGI6B_003910"
     CDS             1123..2412
                     /locus_tag="ACGI6B_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753314.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2342..2986
                     /locus_tag="ACGI6B_003911"
     CDS             2342..2986
                     /locus_tag="ACGI6B_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753315.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3253..3717)
                     /locus_tag="ACGI6B_003912"
     CDS             complement(3253..3717)
                     /locus_tag="ACGI6B_003912"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9753316.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1447 a          433 c          576 g         1316 t
ORIGIN      
        1 atcacatttt tcattatata taaaaataga ctcacaaata aaataaatat atattaaatt
       61 ataacttcat aaacttaaaa aatttcagaa acaaagatac tttaatttaa ttaaaaacag
      121 aaaaattaaa atctttagaa aaaatttaga aatttataaa taatatctta agttttagaa
      181 tttattatga aaatacagta aaaatttata aaaataaaat tctataaaat tgtttctggg
      241 ggtgtattta tatatggaaa tgaaaatagc agtatccaat cttagtaaaa gatttgggaa
      301 aaaacaagct cttgataata taaatcttga aatcgacagt ggtatgtttg gtcttttagg
      361 aagaaatgga gcaggaaaaa ccacatttat gagaatttta gccactttat tatcaaaaac
      421 agaaggttca atacatatgt gtggagttcc tattgagaat actaaggaaa ttagaaatat
      481 ggttggttat ttgccacaag atttttcaat gtatcctaac atgacagtat ataaagcaat
      541 ggattattta gcagttttat cggatatacc actaagaaaa agaagaaaaa ttatagcaaa
      601 gttattaagc aaagtaaacc ttactaatta tcaaaatgtg aaagtaaaat ctttatctgg
      661 aggtatgaat agaaggcttg ggatagcaca agcattaatt cataatccca gagttttaat
      721 agtagatgag ccgacagcag gtcttgaccc agaagaacgt ataagattta gaaatttact
      781 atgcgaaatt gcaaatgata gaatcgttat tttatctaca catattgttg gagatataga
      841 agctacatgt gaaaacgtag ctatattgaa tagtggaaaa gtcatataca atggctcaac
      901 agaagcatta gcaaaactag ctgaagggaa agtatatagt attgaagtag ataaaaaaga
      961 cattgaaaat ataaaaagta gatttatagt tataggaatg ttgactcatg gtggaaaggc
     1021 tatacttagg attatatcag atgataaacc ttttgagaca gcagtaaatt gtaatccaac
     1081 tattgaagat ggctatatgc ttataatggg aggagataat atatgaatct atttataaaa
     1141 gaatgtaaaa atatattaaa gagtatggta tattatattt ttattgtagt tttagttttt
     1201 ttctatattt ctcaacttgg aagttcagtt tcaaatgatg taagtaaaaa tattcctaat
     1261 gatggaaatc cattagttgc tccacaaaaa gtagagaata ttgaggaata ttattctgta
     1321 cacaatcaat atccatatgg tcaaaaagaa tctatgaaaa cagaaaaggt cattccaaat
     1381 gcaactataa acctaattag agaatatcaa aaaaacacat atacaacgta tccaacaggg
     1441 gtagttaagt atataaaatt agaccaaaat aaaacgaaga ccatagaaaa atatatagaa
     1501 gaaataactg gtaaaagtat tagcgaaata aattcacttt ggaaaactga tgaaagtata
     1561 aaaatagatt attctaaagt atctcttgaa agatttgaag aaataatgca aagagtagac
     1621 gacattttag gaggagggtc tgcatatgct tttgacaagt tacaaagatt tggagtagtt
     1681 ccagttacct atgatgaagc tatgaagaat tacaactata caatacaaaa agataaagtt
     1741 agtaatgggt atgcaagaat tttttgcgat tatataggta tagttttaag tatatttcca
     1801 gtatttgtgg cagtttttat gtcacttaaa gataagcgtt caagaatgag agagattatt
     1861 tattcaaaac aagtatcttc atgcaaaata gttttatcaa gatattttgc acttgtcttt
     1921 atgatgatat tgcctgtatt aattttaggt atgaaagatt tacttccttt gattttcttt
     1981 gctaaagata aaggtatatc tattgataac ttagcattta taaaatatac attatggtgg
     2041 atactaccaa ctcttatgat agttacagct gtaggaatga gtattactat attaacagat
     2101 acaccagtag caattattgt gcaattaatt tggtggcttt acagtataaa ttctacagga
     2161 ctcaaaggtg attattcaac aatggggcta atgataagac ataatagtgt attaaatggg
     2221 gatattatta tacaaaactt caatcaaatt atgattaata gaattgtgat aagcttggga
     2281 gcattggtgc ttgttttgtt agctatattc ttatatgagc aaaaaagaag ggggaatctt
     2341 gatgtttggg caaagttttc aaaactgttt agttctaata aaagaaaact tcaaattaag
     2401 cactttaact aatttaatat gttcaattat attgctatgt atggttcctc taatacaagg
     2461 agtagctaat ttagacagaa tttcaacagc tatatgttta gaaaattatg ttgcaataat
     2521 agggatagta atgttggtac ctgtattttc gctagagcaa tcaaaagaaa ttgatgagat
     2581 agtatcttca aaagcattat ctcaagttat tgtacaatta attaggatac tgatgtcaat
     2641 tataggtata gtggtattaa tcataggttt tgtaaatgtt ttaatatatc ttggatgtaa
     2701 ttttccagta gttgagtata ttattggaag tttcgcatca gctatgttta taggaagttt
     2761 aggaatgttt ttctcaaatt tatttaataa tacaatcata ggttatatga tatctatagg
     2821 atatttaatt ttaaatatga tgactaaagg caagtatgtt ggaaaatttt ttataatgtc
     2881 tatgagtcaa ggtagttttg atgagaaata ttggctgata cttggaagtg ttgtgttgat
     2941 aattgcatct ctcttaataa aaccagtaaa aaggaagatt agataataaa atgagagctt
     3001 agataaaata aaggtaatac aaataaaaag tacatttatg aaatataaat ctatttttat
     3061 atacacaaac ctacttacaa tctctttata tatgtagata gatttatata tccgtagtat
     3121 aaaatatata tgtatacaaa attttatcaa aagctctcaa atttatattt caaagacact
     3181 tattgctatg acctacacat atgtattttt tcattttaat tgttgtatta acaataagac
     3241 tattgttata aactattgat acctcataac aaaaaattct ccatctccta aatgcgtttt
     3301 ttgtacctta gtctcaacta ttttaaatcc ttgagactca taacatttta cagctctcat
     3361 gttccaggtt cttacattta atacaattgg tttagtacta tatttatttc tacattcttg
     3421 taatcctagg ccaattattt ctttaccaat tcccattcca caataattcg gtttcatacc
     3481 aataccaaag aatacagagc ttccttcatc taataagttt ataaaaccta tcaattcttt
     3541 attgtcattt ataaaactta taaaattttt tcttttatct tcttttgcta gtgaaaaatt
     3601 cttcttttta atttcctccc atggaggtag attgtatatt gaatatttac cttcatattt
     3661 ccaattagta acttcaatag catgttcttc agtaaattta actttattta aattcatatg
     3721 tcctcctatt aaaatattta aggtaattaa tggtatttta aagttttaga aa
//
LOCUS       DAYAOY010000187         3746 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYAOY010000187 DAYAOY010000000
VERSION     DAYAOY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.194"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1450
                     /locus_tag="ACGI6B_003913"
     CDS             <1..1450
                     /locus_tag="ACGI6B_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFG9753317.1"
                     /translation="KIDSCNYRINSISKNLKMVIFDNRKDDFYISIRGIDIMNILFSL
                     ERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVATKEINYIKMCLENKDTI
                     TDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKVKSRDEKKAERKSKIPH
                     EFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLRDGRWIMLTVFSLTQPY
                     AENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLAGYINSYVVDYRKLMVC
                     VTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILPYDAKTGYQHVIEMYKG
                     IVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAIYKDKHQEDFLENQKLL
                     ISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEGRSVILESIINTKSLGD
                     KIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            1665..2522
                     /locus_tag="ACGI6B_003914"
     CDS             1665..2522
                     /locus_tag="ACGI6B_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9753318.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            2619..3488
                     /locus_tag="ACGI6B_003915"
     CDS             2619..3488
                     /locus_tag="ACGI6B_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9753319.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1597 a          335 c          618 g         1196 t
ORIGIN      
        1 aaaaatagat agctgtaatt atagaataaa ttctatatct aaaaatttga aaatggttat
       61 ttttgataat agaaaagatg acttttatat cagtattaga ggtatagaca taatgaatat
      121 tttattttca ttagaaagaa taagtttaat cctagaaaga tacaaaaagg atagtaaaga
      181 atttgaagat gaagatataa aaaatatttt agaagaaagt agcataaata gtaaatcaga
      241 tgttctagtt gtagcaacga aagaaataaa ctatattaag atgtgtttag aaaataaaga
      301 taccataact gataaagaga tattaggatt tagagattat gttattgctc aggatactaa
      361 aaatataaat ttaaaagaaa tttatagtgt tttagaaaat ttatatgagt ttttagtaga
      421 ttataaaaaa gtaaaatcaa gagacgaaaa gaaagctgaa agaaaatcaa agatacctca
      481 tgaatttaaa cgactatcaa tatataagaa aaactttaat ttaaattcta taagattttc
      541 ttatgcagta aagattgctt tagctacagc tgtagcgggt tttattatgg attatttcca
      601 tttaagagat ggaagatgga taatgttaac tgtcttttca ttaacacaac cttacgcaga
      661 aaattgtata caaaggtcta gaaaaagaat agaaggtaca tttataggag ctgtaatatt
      721 tattgtatta ttttcaataa taaaagatag tacattaagg tcgttaatag ttcttttagc
      781 agggtatata aactcttatg tagttgatta tagaaaatta atggtatgtg taactgtttc
      841 agctcttggt tcagcagtag ttatgggaga cccaaatgtt ttaaccataa gcaggatttc
      901 atatgtagca ttaggagcaa ttattgcatt aatagtaaat aagtttatat taccatatga
      961 tgctaaaaca ggctatcaac atgttataga aatgtataaa ggaatagtta aaaatataat
     1021 tgatgaagtc aataagtcta tagaaaatgt agctgatgtt tactatataa aaaatttatt
     1081 attgatacca tcacttattg aagatagact tatgttgata aatgctatat ataaagataa
     1141 acatcaagag gattttttag aaaatcagaa attactaata agtaatatgt ataatctata
     1201 tataaatgtt aataaaaata aaataaaaga tgaagatgtt gaaaaaatat tgagagatac
     1261 taattatatt tcaaattaca atgtagataa gtatgatgaa ggaagaagtg taattttaga
     1321 gagtattata aatactaaaa gtctaggtga caagataata tgtctaaact tactgcaaac
     1381 tctaaatggt gtcaaagaaa tgtatagaat aagtaatata actaaagttt caataaaaga
     1441 agcagcttag atattaaatt atagaatagc catatattta aaataaagta tatggctatt
     1501 tgttttataa aatactaaaa gaaaataata tataaaaaac aaaaaacata tatttatgaa
     1561 ttttatatga aaatagttaa aatattgatt tttttcattg gttgaaaaaa caatttactt
     1621 aggataaact ataatagtat tcaaatatta tgggggttag atttatgagt aaagatgtaa
     1681 aggtaaagcg taataagaaa tatgatacat ttagtataac tatggaactt gagagacctt
     1741 tactttatga tgaggttgag aacaaagatg ataaaaataa taatagaaaa tttaacataa
     1801 agaataagat atttaaggga ataggtatac tatttgtatt gattttgctt ggctttttca
     1861 tatggataaa taaaacatat gaacctcaaa agctggcaaa agaagctcta gtcagcaata
     1921 gtaaagttga agtaactgta aatgaaaaca tttcatttac accaaaagga agaaaagttt
     1981 ctaaaggttt aattttgtat ccaggagcta aagtagaaat agaatcttat gctccacttg
     2041 caagaaaaat agcagaaagt ggatatgaag ttgtaatcgt aaagatgcca cttaatctag
     2101 ctatacttgg aactaataag gcacagaaag ttatggattc ttacaacaac atagaacatt
     2161 gggtaatagg aggtcattca ttaggaggag ttgcagcatc aaattttgca cgtgataata
     2221 aattaattga aggcgttgta tttttagctt cttatccaat gggtgatgag ttgaaagaat
     2281 taggaaaaaa agttatatct atatggggga gcaaagatgg agtagtcaat tttaaaagtc
     2341 ttgtagaatc aaaacaaaaa cttcctgatg atacaactta tgttgagatt gaaggaggaa
     2401 atcatgcaca atttggagat tatggaaaac aaaagggaga taatgatgct attataagtc
     2461 aagaaaaaca acttaatatt actgccaata gtatcattaa atttttaaag aatatatctt
     2521 aaactaaaat ttaaaaaata taattttata agttattatt taaagtaaat attaataata
     2581 taatagaatg aatattttat tacaaggggg attttaatat ggaatacgat atgccacttt
     2641 acagaccacc aagtgaggct tatagtctta taatacaagt cacactagga tgttctcata
     2701 acaaatgtac gttttgtagt atgtacaaat ctaaaaaatt taaaataaag ccactagaag
     2761 taataaaaaa tgaaattgat atatttagaa gatattataa gcatgtagaa agaatattct
     2821 tagcagatgg agatgcattg ataataccaa tggagaaatt aagagatatt attttatata
     2881 taaaagaagt ttttccagag tgtgaaagaa ttactatgta cggctctcct aaatctatag
     2941 agaaaaaatc tgatgatgaa cttaaagaat taagaaaact tggtgttaaa atgatttatc
     3001 tagggcttga aagtggaagc aatgaagtat tagaagatat taagaaaggt tttagtagtg
     3061 aacaattaat aaaagttggt agaaaagtta aaaaaagtgg gattaaatta tcagccactg
     3121 taatagcagg tcttggagga actaaaaaaa cacatcagca tgcagttgat acaggaaaaa
     3181 tgctaggagc aatttcacca gattatgtgg gtgtacttag tttaatggta gaacctaata
     3241 ctgaacttta cgatttactt aaggcaggag agtttacaat actagaggat aaggctgtgt
     3301 tacaagaaat taaagagatg ataaagaata tagatgctaa tgaaaaaatt gtatttagaa
     3361 gtaatcatgc atcaaattat gctaatttaa aaggtgtatt acctgatgat aaacaacgtc
     3421 taattgatga aattgattac tatctaagta accaaaagct aaaaagagaa gaatatagaa
     3481 gactatagtt tttgatatat caatctatac tgataggtaa atactaagat tatttagata
     3541 caaaaaatca tgctacatta atagttgctt actattgatg tagcatgatt ttttattaaa
     3601 aaacaaaatg gtatattcat atataatata tcaataataa tgaaaatgtg aactagttta
     3661 atagaatttt acctattata ataaaaacag gaatttaaat aataaaaagt aaagtaactt
     3721 tttattaagt aactttactt tttatt
//
LOCUS       DAYAOY010000188         3678 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYAOY010000188 DAYAOY010000000
VERSION     DAYAOY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.195"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(433..1305)
                     /locus_tag="ACGI6B_003916"
     CDS             complement(433..1305)
                     /locus_tag="ACGI6B_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9753320.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1699..2277)
                     /locus_tag="ACGI6B_003917"
     CDS             complement(1699..2277)
                     /locus_tag="ACGI6B_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9753321.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2335..3051)
                     /locus_tag="ACGI6B_003918"
     CDS             complement(2335..3051)
                     /locus_tag="ACGI6B_003918"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFG9753322.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3242..>3678
                     /locus_tag="ACGI6B_003919"
     CDS             3242..>3678
                     /locus_tag="ACGI6B_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9753323.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLK"
BASE COUNT         1154 a          579 c          355 g         1590 t
ORIGIN      
        1 acttaaaaaa tcaagatttc atacctaact actaattcta tgatttttaa atttttcaac
       61 ttctttgtca aagttaaaat aaaatactaa aagacacata ttatagtgaa caaaaaagat
      121 agaaatacat attttaatat aattttaata agatattata tacttaaata caaataaaat
      181 taattttata attgttaatt aaggaaatga tattgtttag agaaataaaa aaagcactct
      241 ccatgaatgg aaatgctttc tttaataaat tagttgacgt agtaaagatg tattcagaat
      301 acatctttat gttttgagtc tcgaaatatt tatttgtatt atttaaacga aaatttattt
      361 tttaagtgat aatcgagtat ttactaataa ggctatgatt ctcaatttca tagccttatt
      421 aatatacaaa ctttactcat acacagaata tcttttccaa atttgctcta aatcatcaaa
      481 atgctgttta atatcttctt tttccttcat cccaacagat atagcaaggg catttgaaat
      541 actaagagct ggaactagag aatctacaaa tgaagccata ttactcttaa ctagaagagt
      601 gttatctgca agtgatgcta ctggcgcaaa taagctatct gttaatgata ttacatgggc
      661 tcctttttct ttagcataat tgacaatttg ataagatttc ttagaataac gagggaaact
      721 aatagcaact attacatctt cttcatttat tcttacaatc tgctcaaaag catctcccat
      781 atccattcta attatatgaa cattgtcaag tattatatct aagtaaaaac ctaaatattg
      841 agctactaca aaagaactcc tcattccaag tatatatatt tttctcgctc ttaaaagttt
      901 gttagaagct tctttaaacg ctctctcgtc aatttcttct agtgtagacc ttatattatc
      961 tatatcactt tttaaaactt tgtttagtat tgcaaaatca tcggaataat catgtgccat
     1021 ttcaactctt tgtactgtag ttagtttatt ttttatgagt tcttgaagtg cagcttgtaa
     1081 tttaggatat ccactataac ctaatgcatt tgcaaatctt acaactgtag actcacttac
     1141 tcctacggtt tcacccaatt tacatgcagt cataaatgca actttatcat aattatttaa
     1201 aatgtattgt gctataagtt tttgaccctt actcaatctc gtatattgag attgtatatt
     1261 tgatatcaaa tgtttactgt ccttcatttc atttgtttct tccatttttt tctcctttta
     1321 gttatataaa ctattttgtt ttattaacac tgtctttttc tctaaaaatt ttatttttta
     1381 tctcaatttc atcttagttt ttatcatagc ttcttaaact cataaaattc taacttttct
     1441 gtaccttata cttcacatag tattttcgat taaatttatt ataccatctt tccttgcatt
     1501 ttttaatatt tatccaatat ttcttttaaa tttttcaatt tttactcatt taaaagttat
     1561 ctatcttatt ttgcatattt agatgtatat tttgttttga gaataaactg ttgtatactt
     1621 attatttaaa gtatttttaa taggtcacac tatatatatt aactcttaat acaatcttgt
     1681 caagtttttt agtatttatt atgctttttt cttttcaatt aaaatcaatt gtggtgggtt
     1741 attgacttga tttaaaaact tagattctaa aacattaaat tcatcttgat ttaaattatt
     1801 gacaaggtta tatatgcaat ctttttcttc ttgacctcca tcatgccctg tatatatggc
     1861 aatgcttatt actccatttt cttttaacat ttctaaactt aactttatag cctttaaagt
     1921 tgtatctggt ttggttataa ttgaatgttt cgctcttgga agataaccca aattaaataa
     1981 aacacatgaa acttcttctt ttatatattg attcattttt tcatgcccat caaatatcaa
     2041 ttttactcta tctgtatatc cttctttctc tagtttcttt cttgtagact ttattgcttc
     2101 ttcttgtata tcaaaagcat atacaaagcc cttttctcca accttctcag ctagatacct
     2161 tgtatcatac ccatttccca tagtagcatc aattactatg tcaccttcat ttataatatt
     2221 ttctaaataa aatttattta tatctgtaat tttagttaaa tactttgcat atgccataat
     2281 actatttctc cctactatta actttatttc attttttctc aatttatttt tatcttattt
     2341 tattgatttt aaatatttta tttcatcttc tgttaggtat ctatattctc caactttggt
     2401 gtcttttaat actattttcc ccacagaaat ccttcttaaa tttaaaactg gatgatttat
     2461 agctttgcac atttttctta cttgtctatt tctaccctca tgtatagtta tttcacacac
     2521 tgaataattt ttttctttat tttctttagt tacccttatt ttggcaggtg ccgttgtgta
     2581 atcttctatt tttagtcctg tttcgaattg tcttatctca tcatcactta taattccctt
     2641 cacacttgcc acatacattt tatcaatttc atgctttgga tgggtaagct tatatgttaa
     2701 atcgccatca ttagtaagta ttaataaacc actagtttca taatctaatc tccctattgg
     2761 atatactctt tcccttacat ctttaagtat gtctattaca ctgggtctat tgaattggtc
     2821 ttttactgta gttatatatc cctctggttt atttaatact atatatatat aattttcact
     2881 tggttttact tttttattat caaattctac tatgtctttt tcttcatcaa tattaaaaga
     2941 taactctttt actacacttc catttacctt tattcttcct ccaagaatta tttcttctgc
     3001 ttttcttctt gatgctattc cacaagaagc tatatactta ttaattctca tacttaatcc
     3061 ttccttatct taaattatgt gtgtttaata tttttatcaa tgttaatttt gctacattat
     3121 tatactatac tagaccttaa ttaaaaacaa gcaaaaaaat tatttaattt tcctaaataa
     3181 ttgtaatttc ttttcacata ctattaatat gtaatagctg actaaacact attttaagga
     3241 agtgattatt atttcaaagg aaaatataaa atattattta atagtcgtgt ttattggaat
     3301 tttatttttt aaatttatta atactccatc agactttata tctagtattg aaggattctt
     3361 aaagtttttt agcccatttt tattagctat tttacttgct ttacttttaa atccacttgt
     3421 gatgcttttt gagataaaat tcaaagcaca tagactttta gccatttttt tatcttatat
     3481 atttataggt tttattttag cctttgccat taggttatta attccatcaa tagctaatac
     3541 attaaataga ttgataaatg aaatgcctat gtatactgat tatatagata gttttataga
     3601 aaaaaatatg tcaaacatat attttctaaa aactttaatt cctcatatac aacatagttt
     3661 agataattta ttaaaaga
//
LOCUS       DAYAOY010000189         3593 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYAOY010000189 DAYAOY010000000
VERSION     DAYAOY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.196"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(77..1924)
                     /locus_tag="ACGI6B_003920"
     CDS             complement(77..1924)
                     /locus_tag="ACGI6B_003920"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HFG9753324.1"
                     /translation="MKPILKWAGGKTQLLDILRKQMPTGCDFSSMTYIEPFVGAGALF
                     LYLLEEDIFNKYVINDINSKLINLYIKVRDDLEELITELTNLKEQYISYEEGSSDREK
                     MYYDIRDRFNILTNEKNIENEDCIKQASYFIFLNKTCFNGLYRENSRGEFNVPFGRYK
                     NPSFFDKNELEKLSMLLNKRDSRGELKVKILNKNYLNLENIIDKNCFVYLDPPYRPVT
                     IGGFNSYNKGNFNDDSQIELSKFYKVLDKIDTKLMLSNSDPKVLDEEDTFFDDLYSDF
                     NIKRVHAKRFINSKGNGRGFITELLITNYEEVGNQMELVKNIHKNKSKDEIFKELLEN
                     LKGTIASWDYYVNWNKALENTRKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVI
                     PIVIASRDNEFKILDPSDEDMFKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQ
                     KNIKNLVDYVFGVEVGLDTNARKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKI
                     SEKWSIEVPTDKSKRKYDFAINNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKD
                     GGFTFCWITDGLGWLESSIPLRDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(2352..2701)
                     /gene="ssrA"
                     /locus_tag="ACGI6B_003921"
     tmRNA           complement(2352..2701)
                     /gene="ssrA"
                     /locus_tag="ACGI6B_003921"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2859..3314)
                     /gene="smpB"
                     /locus_tag="ACGI6B_003922"
     CDS             complement(2859..3314)
                     /gene="smpB"
                     /locus_tag="ACGI6B_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFG9753325.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         1184 a          645 c          341 g         1423 t
ORIGIN      
        1 tcaaatattt tatataatat ataagtctaa attaacttgc aatataaaag cgcctcttaa
       61 tataggcgct ataatgttat aaatttttaa ttatatattc caatgaacct ttatcaacca
      121 tatctaaatt aaatatataa tccatcttat caaatgcatc tcttaatgga atacttgatt
      181 caagccatcc taacccatct gtaatccaac aaaatgtaaa acctccatct tttatatact
      241 tatctaactt agtaaactca tttgcaacag attttaactt agaacctcca ccattataaa
      301 aattaacttc tactaaaaca atttttccat tattatttat agcaaagtca tactttcttt
      361 tactcttatc tgttggaact tctatagacc atttctcact tatttttttc actgtagctt
      421 gtgttaaata actccaatta ttcttttcac acatattctt tacatatctt tctacaagag
      481 tttccatact acttccagtt ctattttttc tagcattcgt atcaagccca acctctactc
      541 caaacacata atctactaaa ttttttatat ttttttgttt taataaatct aaaaacttta
      601 tttttttaag aaattctgta gctaattcgc tttcttcatc acttatactc tttctcttct
      661 taaaattaaa cagtttatat ttaaacatat cctcatctga tggatctagt attttgaatt
      721 cattatctct actcgctatt actattggta tcacactaag aacttctgga tactctttta
      781 ataaattttt agtttcgttt tctatatctt cttttcctat aagatagttt aagatattta
      841 actgaacctc tatctttcta gtattttcca aagctttatt ccagttaaca taataatccc
      901 agctagctat agtacctttt aaattttcaa gtaattcctt aaatatttca tccttactct
      961 tatttttatg aatattctta accaattcca tttgattccc cacctcttca taattagtaa
     1021 ttagcaattc agttataaaa cctcttccat tccccttaga atttatgaat cttttagcat
     1081 gtactctttt tatattaaaa tctgaataca aatcatcaaa gaaagtatct tcttcatcta
     1141 aaacttttgg atcagaatta cttaacatca attttgtatc tattttatct agaactttat
     1201 aaaatttact taactcaatt tgactatcat cattaaaatt acctttgtta taggaattaa
     1261 atcctcctat agttactggt ctatatggtg gatctagata cacaaaacaa tttttatcta
     1321 ttatattttc tagatttaaa taattcttat ttaatatttt tacttttagc tctcctcgag
     1381 aatctctttt atttaaaagc atggataatt tttcaagttc atttttatca aaaaaactag
     1441 gatttttata tcttccaaaa ggtacattaa attcacctcg agaattttct ctatataatc
     1501 cattaaaaca tgttttattt aaaaatataa aatatgaagc ttgctttata caatcttcat
     1561 tttctatatt tttttcattt gtaagtatat taaatctatc tctaatatca tagtacatct
     1621 tttctctatc tgaagaacct tcttcgtaag atatgtattg ttcttttaaa ttagttagtt
     1681 ctgttattaa ctcttccaaa tcatctctga ctttaatata taaattaatt aacttactat
     1741 ttatatcatt aattacatac ttgttgaaaa tgtcctcttc taacaaatat agaaatagtg
     1801 cacctgcacc tacaaaaggt tctatataag tcatggaact aaaatcacaa cctgtaggca
     1861 tttgttttct tagaatatca agtagctgtg ttttacctcc agcccatttt aaaattggtt
     1921 tcatgcaaca cctctctcag ttatattgtt atataattat ataaaataag attctaaaat
     1981 acaagtactc atcagaacac tatatttata tcaaatcttt ccaaaatcta agtattattt
     2041 tatcctaaca cttttcaaaa cctctaaata atatacaata ttctctaata tttacttact
     2101 aaaaatttaa atctatcttt aagtataacc taaaacagat tataagttac gtagttttta
     2161 atataaaata tacttctaca agtaaagctt taaatatcaa ttacttattt cataattaat
     2221 gactatgtac cctcaaaaaa acctcatttt ctatggttgg ggtacggttg gtggatttca
     2281 tcaccaaaat aaaaaatact agtcaataaa gactagtatt tccaagtatt tcatcattct
     2341 tttttcaaaa ttggtggaga cgaggggagt cgaacccctg tccgcaaatc ttttccttgc
     2401 tgcatctaca tgcttagcca acttcactaa tatctcacct cacatagcgc caagtggcag
     2461 gctctatgct tagccagtcc caattaatct acttcaggtc gagacacccc taaagtagtt
     2521 ccctgcataa tgacgtccag tcttagtcgg caggaggact aagagggacg agcagcaact
     2581 aggctgctat cgcgaaatta tcgtctgcgt ttattttaaa gttccccagt ttttacgtga
     2641 cccagagtaa cacgacatgc agcctcaagt tccaaaccca cgtcgaaacc tttacgcccc
     2701 cttgagtagt ttatagtcca gtagactttt cgcaaacttt atcaccattt ttattaaatt
     2761 tttaatgtat atataatata atacttttaa gaataaaaat caatgttatg ttctagataa
     2821 taaagaattt tattctaaaa aatacagttt tatctagttt aatacttacc tgtcatttct
     2881 ctttctatac gtctttgtgc atctttctta gccatatctt gacgtttatc atatagtttt
     2941 ttaccttttg ctaaggcaat ctctacttta actttaccgt tttttagata caaactaagt
     3001 ggtataagtg aatacccctt tatagtagtt gctccaataa gctttcttat ttcatgctta
     3061 tgaagtaata gttttctaac tcttaaagaa tcaacattga atatatttcc ttgctcatat
     3121 ggacttatat gaacttgttt taaaaatact tcgctattat ctacagatgc aaatccatca
     3181 cttaggttaa ctttgccttg tcttattgat tttacttctg ttccttttaa ttcaattcca
     3241 cattcataag tttcttctat aaaatattca tgtcttgctc ttttgtttgt tgctaatatt
     3301 tttttaccag ccatataaat ctcaccttct ttgctagaaa tttattccat aaatattaga
     3361 aatttactaa atatttaatg ttgccaaatt atttgactaa cttgtatgat aacatattta
     3421 ggcattttag tcaatatttc actgatatat taagttagtt aggctatata taattaagta
     3481 atataaatgt atgaaaatag ctaaaaaact acttcgaata tacttaatac taaactaact
     3541 ctaaccagta atactaattt ataaatttca aaacaaaaat attacatatt ata
//
LOCUS       DAYAOY010000190         3571 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYAOY010000190 DAYAOY010000000
VERSION     DAYAOY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.197"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(540..1859)
                     /locus_tag="ACGI6B_003923"
     CDS             complement(540..1859)
                     /locus_tag="ACGI6B_003923"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9753326.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1853..1996)
                     /locus_tag="ACGI6B_003924"
                     /pseudo
     CDS             complement(1853..1996)
                     /locus_tag="ACGI6B_003924"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(2120..2830)
                     /locus_tag="ACGI6B_003925"
     CDS             complement(2120..2830)
                     /locus_tag="ACGI6B_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9753327.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1340 a          515 c          248 g         1468 t
ORIGIN      
        1 ataattacta gttgattttt tctataaata attaaatctt ctacattaaa aatcatttct
       61 tcagaatcta tcacaactaa gtcctcctca aattagtaaa atatttttct ttaatattta
      121 ttactaatat caactttact atctaattag ttatttaatt gttgttttta tgttcctagt
      181 tataaagttt atcaaattta cattttattt ttttatacta tatagaactt ttggtcaata
      241 atgttaccat actaaggtcg catttgcaga taaaaataac tctatttttc taaatttatt
      301 taagatatat catgcaaata atataactat aaaacctatc aacattttaa attcaaacaa
      361 atataaatca cattttttgt tctattattt tattgagtac taaaaaggat gttatcttat
      421 tagcatagca ttcaaccatt tttatcacct ccaaatatcc tatgccttaa agatagcatc
      481 ttctatttta tctttcaata ctactgctcc aactgggcaa tttagagccc caactagaat
      541 tagttcctag ttaatggtat ataaatagta attgtaaact tgtttttatc tgagtatatt
      601 tccacatttc cattatattt ttctactgaa ctttttacac tacttattcc tattccatgt
      661 aaaaaggaat ctttcttact tgtaataacc ttattatttt ttaatacaac ttcatttatc
      721 tttgtatttt cacattttat tacaaataac ctatttataa cagttcctct taatttaatc
      781 tttttttgta tacttgtgtc cttaatctta atacaagctt ctattgcatt atctattata
      841 tttgaaaata tactacatat atctggcatc tctataaaat tacattctaa aaaattaata
      901 tctactagaa aatatatatt attcatatca caaatagatt ttttatcact taaaataaca
      961 tctagaatca tattctgagt attaaatata gtattacatt cttttatttg cttatttata
     1021 tctttaatat acttatctgc aaactcattt ttcccataaa cattctgaat acaaatgata
     1081 tgattattca tatcatgata tagttttctt attttcaact gatattcctg taaatttaaa
     1141 taatatttat attgcatatt cattcttttt tttgtaattt catattctat ctttaaacta
     1201 ttatctttta ttattcttcc tattatgctt actaatgaaa gatttgatag taaaaataca
     1261 gttgatattg taagtattat taaactctct ttagaatcga taatcttatc tttaaaaata
     1321 aattcaaata ttactattac accaataatg tttgctatta atggaataga taaatatata
     1381 caatccctct tgtttatttt tgtttttaat ctgataaact taagcaatgg tattaatact
     1441 attagtaatg atttagatat cattattaac tctaacttca acaaattatc ctctaataaa
     1501 tcattcatat tttttataga attaagtgtc attactatac ttaatccaat actatcaaat
     1561 ccaattaata acatccaata tagcaaactt ataaatacac ttttccattt atccactaca
     1621 taatttattt tatacaatac atatgtaata aatatacata taaataattt aatatttata
     1681 ttaaactcaa ttgtattcaa catagtaatt atcataatag caattactat gctactattt
     1741 agggttaatc tattctttct taactcacta aattcattta ataaaaattt taatacaatc
     1801 cattctatcg ccgtagaaaa tataggtaaa aaaatccaaa atacatttat atttaacata
     1861 atacatctcc caatacatat gttaattttt tctttaaatt agccattcta tatttactaa
     1921 ctggtatatc tttaccatta ataactacag tatttttaca tataaattga atatactcca
     1981 aattaattaa atagctcttg taacattttt aaaaatttat tctttcttaa atcactgaat
     2041 tcatttgata aaaattttaa tacaattcat tctatcgccg tagaaaatat aggtaaaaaa
     2101 atccaaaata catttatatc taacataata catctcccaa tacatatgtt aattttttct
     2161 ttaaatcagc cattctatat ttactaactg gtacatcttc atcattaata accacagtct
     2221 ttttacatat aaattgaata tactccaaat taattaaata gctcttatga catctaaaaa
     2281 aattatattt ttctagctct aattctattt tatccatact atttttagta taataagtat
     2341 catttttagt atgtatagtc aaaacctttt tcctaacctc tatatacata atactattaa
     2401 ttaaaatttt attgattata cctttatttt ctatcagcat aaaattttct ctttttttta
     2461 taatatccga tatacaactc aaaatacttt catttaaatc tccaaaatct ataggcttta
     2521 ataaatatct ataagccctt accttatagc cttcctgtat gtaatcaact attgaagtta
     2581 taaatattat ttcagaagta tcattagttt ctctaattat tttagccaca tccatgcctg
     2641 ttagttgaag cattttaata tctaaaaaaa atatatcaat accttctaaa tttgtttcca
     2701 ataattcttc tcctgaatta aactcaaata gttcaatttg actagaaata ccctctaagg
     2761 actttaatat acactctctt aaaaaagcta tttgtgtaat atcatcctca cagataacta
     2821 ttctatacat aaacacccct ccttagacat tagatctgaa aacaaataaa atatcaattt
     2881 ataaataatt atttagttat acaactaaca tggtcatcaa atactattat tcacttaaaa
     2941 aatcaccccc tatttttagt atgttaacaa caaaataaga agatatctta gatttttaaa
     3001 aacaattaat acttaatgta gttttgtata ctatttatag gtaatttttt atatcatttt
     3061 aaacattaaa attattgatg tttaagaact attatattga tagtaatata taattaatga
     3121 tatcaaaaat ttttaattag acttctctct tattcaagat ttttctatat aaaatctaag
     3181 aataaataga aaactaacat ctaacctttt attttttatt ttcgatctat ttacttgaat
     3241 tttaacataa atctatcttt tagtatatat taatcatgct gaatttgtga tgacatgtat
     3301 acaaaatatc gtttagtatt ttctaataaa aaatattact gaaattaaaa atctacaata
     3361 gcatttaaca agtaaaaaca tgtttacaca aatatataat ttaaggtctt taaaatagag
     3421 tatgtattat tctaaaaatt agttatagtt atcacataaa aatagaaaag aattttcaaa
     3481 agatttaaag aacatatata cttcaattgc agaagatgta atgttaagtg aactagcatt
     3541 cttcgaggaa aaatagactc aaaaaattag a
//
LOCUS       DAYAOY010000191         3566 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYAOY010000191 DAYAOY010000000
VERSION     DAYAOY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.198"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..342
                     /locus_tag="ACGI6B_003926"
     CDS             <1..342
                     /locus_tag="ACGI6B_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753328.1"
                     /translation="SLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTA
                     IIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNISLKKNKCKASTKNK
                     SEKRGVVDVTN"
     gene            329..1084
                     /locus_tag="ACGI6B_003927"
     CDS             329..1084
                     /locus_tag="ACGI6B_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753329.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            1181..2218
                     /locus_tag="ACGI6B_003928"
     CDS             1181..2218
                     /locus_tag="ACGI6B_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9753330.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            3028..>3566
                     /locus_tag="ACGI6B_003929"
     CDS             3028..>3566
                     /locus_tag="ACGI6B_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753331.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT         1336 a          470 c          538 g         1222 t
ORIGIN      
        1 agtcttatgg ctcagttctt gggattctca gatgtaaata tgggtatagg aactttagtt
       61 cttggaattg cctctatcat aattggtatt acactattta agaaatttac ttttatcaaa
      121 gatactactg caataattgt tggctctttt atataccaat tcactatata ctttgcaatg
      181 agtttaggaa tgttatcaac tgacttaaaa ctaataactg ccatagttat aatagctttc
      241 ctagctactg gtaacttaaa tatttcacta aaaaaaaaca aatgcaaagc tagtaccaaa
      301 aataaatcag aaaaaagagg tgttgtagat gttacaaatt aaaaatcttt caaagagttt
      361 ccctaatcca tatggtgaac ctaatactat ctttgaaaat ttatcaatcg atatagaaga
      421 tggtgaattt gtaagtatca taggtagtaa tggaactggt aaatctactt tattaaatat
      481 aatatctggg cttataaaag aatcttctgg tcaaattact ttagatagta ctactataac
      541 aaatttagct gagtataaaa gaactcaaat agtaagtaga gtttttcaag acccaagcct
      601 tggaacttgt ccttctatga cggttagaga aaatttatct ctagcactta ataaagggaa
      661 attattgaac ttaaaaaaat gtcttcgtca taaaacgaat gacttggaac acttacttga
      721 aggaatttct ttggatttaa aaaaatattt agatattaaa gtacaatatc tttcaggggg
      781 tcaaagacaa tcactatcac taattatgtc tagcttagca agtcctaagg tcttattact
      841 agacgaacat acagcagcac ttgacccaaa aacatcaaat gaagtaatag agttaacaga
      901 taaaatcgta agagagaaaa atataactac acttatggtt actcacaact tgaaacacgc
      961 tcttcaatat ggagataggt tgataatgct tcataagggt gaagttgtat tagatgtaaa
     1021 agataaagaa aaaaaagatt taacagttga agaaatctta gaaaaatttg aatatgcagt
     1081 ataagaaatt aaaatcttag ttttaatcag aaatatttat aatctatcat atgaatatat
     1141 gaacaaatat aattattaaa ttttaaggga ggaaaataaa atgataaaaa gtaaaaaaat
     1201 attaagttta attatagcag gagtacttgg tgtatcaatg cttactggat gttctcaaaa
     1261 tgatggctcc aatgcttcaa atgaaaataa agaaacagat agcaaaaaac aaaaaaatat
     1321 aggtattact caattagtgg aacatccatc tctggataaa gcaaagaaag gattcatcaa
     1381 agcacttgaa gataaaggct ataaagatgg agataatata aaaatagatt tccaaaatgc
     1441 acaaaatgat atgcctacta cacaaagtat tgctagtaag tttgtatccg acaaaaaaga
     1501 tttgatatat gctatatcta ctccatctgc acaagcagct tataatgcta ctaaagatat
     1561 tccaataata atgactgctg ttacagaccc tgtagaagct ggacttgtta aatctcttga
     1621 aaaaccaggt ggaaatgttt ctggtacatc tgattatctt tcaattgata aaacattaga
     1681 attggttaaa acattgactc caaaagcaaa gaaaataggg gttatataca atactagtga
     1741 agttaattca aaaatccaag ttgattctct acatgattat gctaagaaaa ataattatga
     1801 agtagttgaa aaaggaatca gctcttcaag tgaagttaac caagctattt ctagtttagt
     1861 tggtaaaata gatgttttat atgtccctac tgacaattta atagtttctt ctatgccaat
     1921 agtttctaaa gttgctaatg aaaacaagat tcctataata gcctctgaag aaggttctgt
     1981 atcttctggt gctttagctt gttgtggaat agattatgaa aaactaggtt ataaggctgg
     2041 agaacttgct attgaagtat tagaaggaaa atctgttggc gatataccag ttactacatt
     2101 agatgaaact gaaataataa ttaacgaaga tacactaaaa gcacttgaca tgcaaaagtt
     2161 atcagcagat aatataaagt atataaagtc agatgaaaat gcaaaatcta caaaataaaa
     2221 cttattaatt tcaaaaaata ggtaatatga agtaggtaat atgaaaactt tatataataa
     2281 gtactcttat ccaccctaac cctttgaaaa ttgtgcttat tgtagcaatt tgaatattgc
     2341 atataaagtt attcattttc cataaaagtc gtctatattg acgactgatt atgaaagtta
     2401 tataactttc taatcactac ttgagctgtc caaaagaatt ttaatattct ccagacagct
     2461 tttttttatg atatttgatt gatatacatg tctagttatt ccatatttag taaatatttt
     2521 gataaaattt ccacattcac ttataactta ggataacgtt ttttttcaaa caatacatta
     2581 atttaaaagt attctgagta tacattattt agtattttaa ttaaatttag cagttgactt
     2641 ttattattgt ataatttata atttataaca agtcgagaat acatataatt ttctgactaa
     2701 ttaaaatatt aaaagggaga gataattatg aatactttat taaacaatag aaaatattat
     2761 tattattata gccactattt ttagggtttg tacctacgtt ttaacataga tacagacccc
     2821 ttaagactac taaaattttg ggattgtatc tatgttggct atattgttta taaagttttc
     2881 atctaactat ataagagcca catagattaa actatgtggc ttattcgtgt ttttaccaca
     2941 tagttttgta cactatgtgg tttttttata cacaaaatta gcaaataatt aaaattataa
     3001 aaatattttc aaaggagtgt tttatttatg tcaggtatta tatctgtaat gacgcaaagt
     3061 ttaattttat cgattatggc actgggggtt tatataactt acaaaatctt agattttcca
     3121 gatatgtctg ctgatggtag ctacacaatg ggtgcttcta ttgtagcatt ttctcttact
     3181 aatggtattt ctcctgtagt agcaacttta atggctatct tatgtggttg tactgctggt
     3241 cttgttacag ggattcttca cattaagttt aagatatcta acttactttc tggtatttta
     3301 gttatgggaa tgctatactc tatcaattta agaataatgg gaaaatcaaa tattcctcta
     3361 tttagcttta aacacttatt caatggggaa atatctccta tagttttagc cttagctttt
     3421 gtattcatct gtaaagtcct tttagattta ttcctaaaaa caggtcttgg atacacttta
     3481 aaaggtgttg gtgataactc tcaaatgatt aaatccctag gtataaatat cggttctata
     3541 aaaatactag gacttatgat ttcaaa
//
LOCUS       DAYAOY010000192         3538 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.199, whole genome shotgun sequence.
ACCESSION   DAYAOY010000192 DAYAOY010000000
VERSION     DAYAOY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.199"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            433..2244
                     /locus_tag="ACGI6B_003930"
     CDS             433..2244
                     /locus_tag="ACGI6B_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9753332.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2332..2913)
                     /locus_tag="ACGI6B_003931"
     CDS             complement(2332..2913)
                     /locus_tag="ACGI6B_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753333.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1356 a          353 c          490 g         1339 t
ORIGIN      
        1 gtaatatacc aaaaaaaagg aatatattta cataatatga taaaataaat tcaaagagtt
       61 aaattgtagg taaatagtat tttaattatt tattaaaata tagattatgt atatgccaaa
      121 tttaaaaaga gacataaaga cattatacaa atatattatt ggggtgatat tttgtaagaa
      181 aagaagtgat gcagtaaatt aattgagtaa tttaaaaatg atttaatgat aatgttgatg
      241 aatgtttaaa aagaaatttt agaaaaacgt atattgctgt ttgtgttgga ggtgatgaat
      301 ttgtagaatt tatagggtat aaatgattaa actatttaag ataaatacat atcactgtaa
      361 aatattgata atcatttttt ataagagatt gattatttgc aaattatata tattacaaat
      421 gaggtaacaa taatgaagaa aaaatatgat tttttaattt attctgctat ttggctagtt
      481 attattattc tttttatatt ttctctatat catagtatag agcatattaa aattataagt
      541 cacactggta ctattcgcag tgaaactcaa aaggttgtta agcaagaatt gaataatgaa
      601 agaaatgatg acttgataaa acgactggat aatattctga taaagctaag aacaggtaat
      661 ggagaaaatg gatttcaaag atgtgataat aaagaatttc aacaaaaact aaatcaaatg
      721 gattctatgt gggaatctat gaagaaagaa attataaaag ttagaaatgg tgcttcaggt
      781 gataaattat ataaattgag tgaagagtat tctgtgttgt ctaatcaaat tgtttttata
      841 tctgaaaaac actctaatgc aaaattgtat tcttttgcta ctgcactatt tatttatctt
      901 atattctcaa caataagtct tttaatttgg gaatattata ataaaaaaag gtttaaaaga
      961 atattttata ctgataattt aacaaaaata aaaaatcagg ttgcatttga aaacagagcg
     1021 attgaaattt tgcacaatgc atctaacaaa gagtatgtac tgctaaatat tgatattgac
     1081 aattttaaat atattaatga tacacatgga tatgaatatg gagataaagt tctaataata
     1141 gttgcaactg ttctttctaa aacttttaat ataaaagaaa cttgtgcaag aatttgttca
     1201 gataattttg taatacttgc aaaatataga gattctcttt tagaggatat tcgggaaatg
     1261 ctaactaatg ctataatctc tgaattagat atgaatgtaa ctcaaactat atcttattgt
     1321 ataggagctt acttagttga aattgataat ttaggttata aatctatcaa ttctatgatg
     1381 gataaggcaa atattgcaca caaggtaagt aaaacaagag gaatttcctc cacagtatgg
     1441 tataatgaaa atctgttaaa acagttacaa atggaaaata gtatttataa ttatatgtat
     1501 aaagcgatag aaaatgaaga atttcatatg tatttgcaac ctaaatttca aatatcttct
     1561 ttaaatgttg tgagcgcaga agctcttgta agatggtttt ctccagaact tggtttttta
     1621 tcaccagatg aatttattcc attgtttgaa aaaagtggat ttattattga attggacttt
     1681 tatatgttaa aaaaagcgtg ttcatttgta aaaaaaacgt ttatgaaaaa aaatcaatat
     1741 acatatccga ttgcagttaa tttttcaagg gttactattt accaaaatag tttttatcaa
     1801 aggtttttag atactgttaa agaatatgaa attcctttta agtatataga gatagaggta
     1861 actgaaagtg cttttaatga aatatctcaa ccagttattt caattttaga ggaacttaaa
     1921 aagttaggat ttttaatttc tatggatgat tttggatcag gatactcttc attaagtctt
     1981 ttatgttctc tatctattaa tggattgaag ttagataaaa gcttacttaa agaaacattt
     2041 aaccgggaga aagtatatag tattattcag tgtattattg aaatgtcaca tcgtataggc
     2101 atgtctgttg tatgtgaagg tattgaaact aaaaaagact tggaattttt aaatacagtt
     2161 aaatgtgatg taggacaagg gttttatttt tctaaaccga tagaagaaaa agaatttttt
     2221 aacaaatatg taactaaaaa ataatataat tatttttatc agtctataca ttgagtcatt
     2281 ttatatctcg ttaaatgact caatgcactg ttatgtattt taaaatataa attatttaaa
     2341 acgtttttta tacttatata gaaattcttc tatatttttt gattgttctc ttatttcaac
     2401 tgcttctata tctagtaaat aaagtataga atcatttgta agttcaacat attcaccatc
     2461 ttctgaaccg agtgcatgtc tctcaatttt taatcctttt tcttcataat atttttttgt
     2521 ttctatatat aatgattctc gcttttgtat gtagtctttc acttcattat ttggaaagtt
     2581 atatgaatcc actaatgttt cgccatttat aaaagttttc tcggtagcaa ctttttcatc
     2641 ccaacaatgg atttcttccc aagtaaaaaa tatctcagtt ccatctggaa tgaatgaaaa
     2701 cctggtaata atatataaat aattattgaa atttacttca taatattcag gaatagcttc
     2761 aaattccata tagtaatttg tataattaaa gttactacta tcttttttta caaatggtat
     2821 tttctcaata aatttatata ttgattgttc atttttaaaa attcctatat catagcttct
     2881 ttcaaattga ttaattttta ataaatatga catatattct ccttatttgt tgaaatataa
     2941 tttttataac tctactaatt ttaaacattt ttaaataaat atttgtatat accttatata
     3001 atattatagt aattagtttt aaatcaatag aatagtatgt atttatctaa tgcggcaata
     3061 taatttaaag ctggtgttgt agaaaaacta agagtgaata tagcaaagag aagcaatagt
     3121 attttaaact ttgtctctct tattttttga aaactaacat taaaacataa ggtataaata
     3181 atattatcat ataaatgaaa acaaatttga atttttctct ggtcgatgta tatagtaagt
     3241 ttgttgatat gagaaaaatt attattgata atatacatgc atagaaagga aatggtatat
     3301 aatattttct taagtttctt ttttaggcat ttctattggc attttaggct atagtactaa
     3361 atacattaac caacaaaata atgaaataaa tatatatata gaaaacttaa ttttcgtggg
     3421 aagaaaaaaa gcaataatag caagtattat tagagaataa cgaataatac gagtaacatt
     3481 tctttctttt tctatgatag ttaaatccca gtgatttttt ataaattcaa ggtctttt
//
LOCUS       DAYAOY010000193         3513 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYAOY010000193 DAYAOY010000000
VERSION     DAYAOY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.200"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            230..607
                     /locus_tag="ACGI6B_003932"
     CDS             230..607
                     /locus_tag="ACGI6B_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753334.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(1044..1307)
                     /locus_tag="ACGI6B_003933"
     CDS             complement(1044..1307)
                     /locus_tag="ACGI6B_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753335.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            1623..2198
                     /locus_tag="ACGI6B_003934"
     CDS             1623..2198
                     /locus_tag="ACGI6B_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753336.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            2296..3294
                     /locus_tag="ACGI6B_003935"
     CDS             2296..3294
                     /locus_tag="ACGI6B_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HFG9753337.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT         1407 a          295 c          534 g         1277 t
ORIGIN      
        1 aaatgtatat attatgtcaa atattagaat tttaatttga taatctgtta tacttataga
       61 atagatattt atctttatat taatagattg tgtgtttaat tttgaataaa aatataaatt
      121 ttttgtatta ataaatttgg atgagatatt ttaaataaaa cctttgactc gaaaagtcta
      181 ttaatatagt atttaattat gtagaagttt gagaggagtg taaaatcata tgactattac
      241 taagatacca ccagcagaat taaaagtcat gaaatttatt tggagttcag attttacagt
      301 agcatcaaaa gatgtcattg aagctatgga aaaactatat aattggaagc aaactacaac
      361 actaacactt ttatcaagat tagtaaataa gcaattctta agtgcaagaa aaattgatag
      421 acatacacat tatacagttc ttattggaga agaagagtat ttacattttg aaacaaaaac
      481 ttttttagat aatatgcatg gaagttctat tgagagtttt atgaaatcct tgttcaaaaa
      541 gggggtaaat gaagaagaat tgaactctgt tgaaaaatgg gttaaggata ttaaaaaaga
      601 tgaataattt ggtgtagtta attatcataa aataaatagt ttttacaata attagttaaa
      661 tgaatcaaat ataaagtttt atgtaaaatt tgtgattttt ataaatattt ttgtcttgtt
      721 atttaatatt aatacaataa gtttgatgta aaatcaaacc tgtttgaatt taatttatat
      781 tgggtttgtc attaatatta ttttagtttg atattgtata cgaatttaaa agtcagaatt
      841 tttgtatatt aatcatgtaa attaattgtt ttagatataa agggcataat tttatttttg
      901 ttttgattat ttctagggta agataagtat acaatagatt gtattaaaca tgcttatatt
      961 gatagatttg agataaatat tgagatatat taataattat taatataaaa aagttcctag
     1021 tttttaggaa cttttttatt tgattaatac agagtatgag tttttgcttc agctatagct
     1081 cttgctgcac ttttaggttt ttcagtattt tcttttttgt aaaatttaat cacttcttct
     1141 aaatctttat tttcaatatt atgttttttg ttttcaagaa tataatttac aattgattca
     1201 tttatcaaat ctgtcaaaat attattttcg tcattagtta tcttagacat ttcttgtaat
     1261 aaaatttcat taacttctac tgtaatttgt tttgtatttt ttatcatatc aaccacctct
     1321 taagaaaatt atatcatctc aaaaaaataa attcaatatg ttttaaaatt atagaatttt
     1381 aattcttaaa ttctaaaaaa gagaaaatta gataaagtag ttttagattt taaaagatta
     1441 ataaaaaata tttaaagata aaagctttat tctatatgat tctaagatag aggatttata
     1501 agtgtttaag gtgaattaaa tataagacaa cttcttaaag caaatgggtg tggtatttaa
     1561 atctaaatgt agacatataa tattaaatat gctaatatat caaaataata aggggggaat
     1621 ttatgaagct taaagaagca ttgattacat gtgttgtatg tagtgtatgt ttaactccat
     1681 tattggtaaa tggagaaaca aaaatagatg cagatcattg taaagaatct atagtaaata
     1741 tagatactat agtaaagtca ggtaaaagct ttaattccaa gtataatcaa ggtatggaag
     1801 attataaaaa tagtgatgag tataagttga aagtacaaaa ggagaaacta caaaatcagt
     1861 taggtattaa aataaagaag ttagtaccta taacttttga agttagttat tacacaagtc
     1921 ttaatcaaga aaatggatat ggagcgataa ctgctacagg agatgattta gaagatggtt
     1981 ttgtagcaaa caatcactta agttttggaa ctaagattat tgtagatgga aaattaaaaa
     2041 ctgttatgga tagggggtct agtaagtatt ttagtaattc taatgcaata gatgtatttg
     2101 tgcccaaatg gagtaatgaa agtgaatcag agtactatga tagagtcaat aatatgggaa
     2161 gacattacaa aaaaggctac attattgttg aagaatgatg ttctttgaag taatatgtat
     2221 tgattgtagt atttaatata tatataataa atataaaact attagtacaa tgagatatat
     2281 tactaggagg gcaatatgga acaatcgttt ataaatggaa aagtaaactt attaatagaa
     2341 aattacaaag cattaaatga agttaaaggt tcttggcaaa tgggattgat acaacatagt
     2401 tgtgcacttg catttaccct taagaacaag agaataagtc ctagattagt ggaagaacga
     2461 atagagctta ttaaaaaaaa tactggatta ttctctaatt ttagaggata taatatgttt
     2521 tatatggcaa cactactttc gtttgaatca aatccagaaa gtagttttaa gatgatttta
     2581 gatatataca aagaacttaa aagtgaaaag ttttggggcg atacatattt accacttaca
     2641 gcatcaatag tttatgaaaa tagagagaaa atggattatc ttacatgtat atcaaaaatg
     2701 aaaataatat atgattacat gaggaaaaaa catccatttc taacttctag tgatgactat
     2761 tgtaatatag cacttattgc aattcattca aaaaatttag atgaagattt agaatacata
     2821 gaaaaatgtt atgaatttct aaatgaaaat ggtttttata aaggaaatga tttacaagca
     2881 ttatcccaaa ttcttttatt tagtgatgat agaactatgc ttaaatgtaa aaagactatt
     2941 gaacttaaaa aagcatttaa agaaaatgat tgtaaaatga attattatgg atatccaata
     3001 ataggagcaa tttcattatt agattataga gaagatgaaa taatagaaga gataaaaaat
     3061 gtttcaaata aacttaaaga agaaaaaggc tttggaaatt ggtctttagg aaaaagtaat
     3121 agagtaatga ttagttctgc tattgtagct tcaatttatg cagatttgat acgaaatgag
     3181 aataatatag gctctataac taataatata tttttaaata ttgttgttgc tattcaaatt
     3241 gcatgtgtaa tggcagcaac aagtgcagct atagcatctt cttcatcaaa ttaataagat
     3301 ttattgaagt tggatatatt aaatttaata atacaattta tatgtttaaa aagaagatag
     3361 tattacattt taattaactg tgatattata taatatgaaa catataatta atatatagat
     3421 tttaggcaat aaatactaca tacaatatag ggaaagtaga atagtacatc tactttcctt
     3481 ttttgattga gtaagttatt ttggaaaaaa tta
//
LOCUS       DAYAOY010000194         3383 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYAOY010000194 DAYAOY010000000
VERSION     DAYAOY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.201"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            33..776
                     /locus_tag="ACGI6B_003936"
     CDS             33..776
                     /locus_tag="ACGI6B_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFG9753338.1"
                     /translation="MVEVDKKPYFVATDIAKCLGYANTSKAINDHCRWVTKSYIPHPQ
                     NENKILEVNTIPESDMYRLIVNSKLPNAEKFESWVFDEVLPTIRKTGGYIHTTEDMTD
                     DEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADAVASSDDSILVGELAKLL
                     KQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLKIMETKKRAIYNPDGSTK
                     ITKTPKVTGKGQIYFINKLKLVNQLSMLS"
     gene            836..1033
                     /locus_tag="ACGI6B_003937"
     CDS             836..1033
                     /locus_tag="ACGI6B_003937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753339.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNTLNENEQSILS
                     SKDILDFCKEALKYNLVPTFV"
     gene            complement(1020..1220)
                     /locus_tag="ACGI6B_003938"
     CDS             complement(1020..1220)
                     /locus_tag="ACGI6B_003938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753340.1"
                     /translation="MKVGDIMQFSKDILHTLTLEILKEKYDFKSSSEEELLKHYHEIF
                     LKLSEVNNSFSKGDGLSVFKQM"
     gene            1273..1470
                     /locus_tag="ACGI6B_003939"
     CDS             1273..1470
                     /locus_tag="ACGI6B_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9753341.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            1500..1943
                     /locus_tag="ACGI6B_003940"
     CDS             1500..1943
                     /locus_tag="ACGI6B_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9753342.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            1944..2090
                     /locus_tag="ACGI6B_003941"
     CDS             1944..2090
                     /locus_tag="ACGI6B_003941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753343.1"
                     /translation="MNVRVLIAYVQFCNDKQIKASFEGLRKYNRDIELVNLYLPSKLK
                     IKGD"
     gene            2093..2410
                     /locus_tag="ACGI6B_003942"
     CDS             2093..2410
                     /locus_tag="ACGI6B_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753344.1"
                     /translation="MKSLTIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDVLDEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEEIERDRPID
                     IQI"
     gene            2488..2997
                     /locus_tag="ACGI6B_003943"
     CDS             2488..2997
                     /locus_tag="ACGI6B_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFG9753345.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKKVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            3007..>3383
                     /locus_tag="ACGI6B_003944"
     CDS             3007..>3383
                     /locus_tag="ACGI6B_003944"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFG9753346.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDAT"
BASE COUNT         1509 a          359 c          587 g          928 t
ORIGIN      
        1 tatttgaaaa attggaattt ggtcaaataa gaatggttga ggttgataag aaaccatatt
       61 ttgttgcaac agatatagct aaatgtttag gatatgcaaa cacaagtaaa gcaataaatg
      121 accattgtag gtgggtaaca aaaagttaca tacctcatcc gcaaaatgaa aataagatat
      181 tagaagtaaa tacaatacct gaaagtgata tgtatagatt gattgtaaat agtaaactac
      241 caaatgcaga aaaatttgaa agttgggtat ttgatgaagt tttaccaaca atacgtaaaa
      301 ctggaggata tatacataca acagaagata tgacagacga cgaaataatg gcaagagcat
      361 tacaagtagc tcaaaagaca atagaaaaga aaagcagaga gatagaagaa aaagataagg
      421 taattcagtt acagcaacca aaagtattgt ttgctgatgc agtagcatcc tcggatgatt
      481 ctatattagt tggagaactt gcaaaactac ttaagcaaaa tggcattgat acaggcgaga
      541 aaagattatt tgcatggctt agagataatg gttacttaat aaaacgtaag ggtgaagatt
      601 acaatacacc aactcaaaag agtgttgact taaaaataat ggaaactaaa aaaagagcaa
      661 tatataaccc tgacggaagt acaaagatta ctaaaacacc aaaagtaaca ggcaaaggtc
      721 aaatatactt tatcaacaaa ttgaaattag taaatcaatt atcaatgtta agttaaaata
      781 taacagcact ttgaaaacta aatacagaat attttaaaaa ggagtgtgta attaaatgaa
      841 aatttcattt gaaagtgtag tgaacaatgt aaataatgaa acggaattaa tattatcaaa
      901 agaagagtta caaatagcta aaaggatact taatacattg aatgaaaatg aacaaagtat
      961 cctttcctca aaagatattt tagatttttg caaagaagct cttaaatata atttagtacc
     1021 tacatttgtt taaagacact gaggccatcg cctttagaaa aactattatt gacttctgaa
     1081 agtttcaaaa agatttcatg gtaatgtttt aaaagttcct cttcagaaga acttttaaaa
     1141 tcatattttt ccttaagaat ttctaaagta agagtatgta atatatcttt actgaactgc
     1201 ataatatcac caactttcat aaaaagatat aggatatatc ctacaaatat agtatatcaa
     1261 aggaggaaaa taatggcaat taatgacaac ataaataaaa ttttaaaaga tagagattta
     1321 aaagcatgga aattagcaaa agaaataggt gtagattcag ggaatttata tgcaatttta
     1381 agaggagaaa ataaaaatcc aactatagat acattaataa aaatagccga ctatttagac
     1441 attacattag atgaactagt tggaagataa aaattaaata cagaatattt tgaaaaggag
     1501 tgagtaaatt gggcaatata tctaatttca atttagataa acaagaagat aaaagtttta
     1561 atgacctaga taatatatca atttgttttt cagaaggtat ccgtaaagtt gtagaaatga
     1621 atttaaacaa ctataaaaat aaaatctcaa agtacttaaa tgaaacttcg aaaatagaat
     1681 tattagaacc aaaagaacta acaattgtta taagtaaagg ttatcccgat tatcttatgt
     1741 ctgttgaaga agcaagtaaa agattgaaaa tagataaagt atttggatat gagttaataa
     1801 aaaatgggct tttgaagtca gttgatatag gggcaactaa agtttctagt tatgaattag
     1861 atgattttat aactagaaat caaggaaaaa acatcaaaga aatgcttaga gaaatgaaag
     1921 aaattagaga gggggtgatt tagttgaatg taagagtact aatagcttat gtacagtttt
     1981 gcaatgataa gcaaataaaa gcaagttttg aaggtcttag aaaatacaac agagatatag
     2041 agttagttaa cttgtattta ccaagtaaat taaaaattaa gggggattaa tcatgaaaag
     2101 tttaactata gtaagaaatg cagtagagca acaactaaat agagctaatt tagaaataaa
     2161 taaaaatgag gaactttata caaaacttag aaaaaaagaa aaaagagatg tattagatga
     2221 aatagagtta agtaatgctt taagagaaaa aagtgtaaat gaaagattaa aaatatttgc
     2281 tgagtcatta ctaaaaatta tagatacaca aattgaaata aaagaatatg aagaaagcga
     2341 ggattacaag atatttgaat taatttcaga agaaattgaa agagatagac ctatagatat
     2401 tcagatatag aaaagagcca ttaggagtgg ctcgattcca aaataatata aaatataagc
     2461 taattatagc acaaacggag ggaaattatg agtactttat atgaattaac tacagattta
     2521 ttagaaatag aagaaggttt aacagaaaca acaggaaatg aagctgaaaa actagaggaa
     2581 ataaaagaaa taataaaaca agagatacaa aataaaaaca ctagaatagt gtcagtaata
     2641 ttaaacattg acagtgatat aaactctata gattcagaga ttaagaggtt acaagagtta
     2701 aagaaggtta agaagaatac tcttgataga ttaaaaagca atataaaaga ctgtatggaa
     2761 ttacttggga ctaaaaaagt agaaacagtt ttaggaaata taagtataag aaagtcagca
     2821 ggtagcttag tcatagaaga tgaagaaaag atacctgcta tatataaaac agtagagcaa
     2881 gttgtaaaag tagataagaa cactattaaa gattttatta aaaaaggtca tgaggttgaa
     2941 ggttgcagga ttgaatatgg aactacacta acaattccaa aagctaaaaa agagtaggtg
     3001 aggaccatgg aaattaataa tatttacatt aaattgatgg atgtaagagt taaatttagt
     3061 aagttgaatc taaagaaaag tggagaaaat aagttcgcta acttcaagta ttttgagtta
     3121 gcagactttc taccacaagc aactggatta cttgaagaag ctaagctatg ccctatagtg
     3181 acctttacaa atgaatatgc aactctaaca ttaattaatg gggaaaaccc atcagaacag
     3241 attgtattta cttctcccat gagagattta caacttaaag gttctaatga attacaagca
     3301 ctaggaggta tagaaaccta tcaaactaga tatttgtaca ttcagttact caacataact
     3361 gaaagtgaca cttttgacgc aac
//
LOCUS       DAYAOY010000195         3309 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYAOY010000195 DAYAOY010000000
VERSION     DAYAOY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.202"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            487..1167
                     /locus_tag="ACGI6B_003945"
     CDS             487..1167
                     /locus_tag="ACGI6B_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9753347.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
     gene            1615..1899
                     /locus_tag="ACGI6B_003946"
     CDS             1615..1899
                     /locus_tag="ACGI6B_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753348.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(2670..2885)
                     /locus_tag="ACGI6B_003947"
     CDS             complement(2670..2885)
                     /locus_tag="ACGI6B_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9753349.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(2896..>3309)
                     /locus_tag="ACGI6B_003948"
     CDS             complement(2896..>3309)
                     /locus_tag="ACGI6B_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFG9753350.1"
                     /translation="SLLGVAGIIFLCMIPMFLYDFSSDKVIALIARILYFSDYFIVIL
                     FLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECINGYNLNTNNIRIFGTDSGGI
                     SPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         1389 a          286 c          442 g         1192 t
ORIGIN      
        1 attataaaaa taaaagtgat atattgtgta ataatcgaaa ttttatactt tggttaaaat
       61 aattttatat atagtgccta atgtataacc taatgctcgt agacaataat gtggatatta
      121 tcaaatttat ttttataata gaaattttaa tttatatcta tattaaattg ttaaagatat
      181 aattatgaga ataaaaaata tagctttgaa ttatataatt gtatatgctt atctttatac
      241 agtaaataaa aaatcaaatg aaataccaaa taaagtgtat atcttaaaat aacttactag
      301 agatacaagg atgctatgtg aattttgaag tgtgcagttt agataataaa tatatataaa
      361 taataaagaa aatggattta tagtatttaa aatttcttat aagggtaaaa aatgctatta
      421 gaaaatgtac taaaaatatt tttccaattc tttatagaaa aataatgata tttaggagag
      481 attgtaatgc aaattgatgt attgataatt gatggagata agaatagttc tcaagagctg
      541 aaaaattttt tagaaggaaa ggggataaat gtctatttag catataattg taaagatgct
      601 attataaaaa tatttagcaa gaaatttgac ttaatatttt tagagataat tttagttgat
      661 ggtgatggtt gggatttttg taaaaaaatt agaaatataa ctacttgccc aattattcat
      721 ataacttata taaatgaaaa tcaaagtatt ttaaatgctt taaattctgg aggagatgat
      781 tatttaataa agcctttaaa tttagaaata ttatatgcga aagttaaagc tattttaaga
      841 agaagagatt tatttttaaa taataataaa aataatataa aactagaaga ttataataga
      901 gctagtggag taataaagtt agaaaataaa atgataaagt taactccaac agaaaataga
      961 ttattaaatt actttataga gaatgctgaa aagactctaa ctattaagga gatatatgaa
     1021 catgtatgga tgaatgagta tttagatgat aattatagtg tagtggttgc tgtgagtgga
     1081 ttaagaaaga aaatagagaa agattataaa aatcctcaaa aaattataac gataagagaa
     1141 gtagggtatt actttaacaa gatataaagc tttataaaca gaatgaatga taaggcatag
     1201 ataatatgtt agtatattat aagagttttt gttctaacga tatatttgta aaattagatt
     1261 taatttactt aattaaatta tagcagtaga ataaatataa aaaatgtctg aaatattata
     1321 gacatcagta aaatatataa aactataata taagtcgtaa taataaaaca aatgaatatt
     1381 aattaataaa aagatagtag ctatttttat atagctatta tttttttatt gttgtggatg
     1441 aaaatatact tatattttac taatatacat ttaaaaatta taatttaaag cattaaaaaa
     1501 taaatttgta ttttgtttta tataaagtaa tttgaagaat atggaaaaac ttgattagta
     1561 cattattaat tagggtataa aatggtgtaa atagaatgat aaggggtgga tgaaatgctt
     1621 gtatataata aaagttttta tcctaatgac atatttccaa gattagattt ttcaaaaata
     1681 aaaaaacagt taaaattgat agataatgac ctgtcagatt ttggaagcat atgtataata
     1741 gaaaaagaac attatacaat aagtgtaaac agtataggtg aaataaatgt gtattatgat
     1801 ttagagtacg aaaataaggt gtatagaata gtttatgaga ttgaaaagtt atttaaatct
     1861 caagttggaa ggtttagcat atctacatac agaaattgat aattaaaaaa gtagaaatta
     1921 aaaaacttaa tactaaagat atagataaat atatgaggta acagctagag agagttgtta
     1981 ccctttttaa atactaagta tatattattc ttattaattt tttgtaaatt cactaaaaga
     2041 cctttttata gaatattatt ttcttttttt attaaatagg taaatcagta aaaatgcaat
     2101 tccagttcca attattattc caatgatatt ttctataatt aattttttta atataaaagt
     2161 aaataataaa aatgctatac ataaacctaa aatatatcct ttaaaatagg ttgaaagttt
     2221 tcttttcata taatcaccaa cttgtatcag atttagttta cttaattaga gtataacagt
     2281 gttgtaactt caaagagtat ctaaaagatt acaaatgtta gataaaatca tcaataatgt
     2341 aatttataac acgtagctat aaactatgca ataatataaa tggaggtata agtaaatgaa
     2401 aaagttccat gttttgctaa cagtttaaat aaatattagg tagaaaagaa tgtatatagg
     2461 aatctgtggt aaaattatca atttaaaagt attatttagt gtgatacagt tcttataggt
     2521 aatggataac aaatagataa aggaacagtt tctttcttag aacatatttt gttatgactt
     2581 taaacaaagt taatattaat gtcaataata tagtaaaaga ataaaaatca tatagtataa
     2641 taaaaaagag tgtttctaaa cactcttttt taatcatttt catcttctac atattcaagt
     2701 atatcacctg gctgacagtc taaagctttg caaatttcgt ttagagtggt aaatctaata
     2761 gcttttgctt ttccagtttt taaaattgat aaatttgcat ttgtaatatc tattttttct
     2821 gccaaatctt taagtgatat tttccttttt gccatcatta catctaggtt aactactatt
     2881 gccataacat cacctctata tcgttaaatc attatcttct tttattttta tagctttatc
     2941 aaatgtttct gcaattacaa agcacattag tgcaaatata aaagagataa tcataagtgg
     3001 tgagataccg ccactatcag ttccaaaaat tcttatatta ttagtattta aattataacc
     3061 attgatacat tcaattatag tgtttatcaa taaacaaaat cccattacct taaacctttt
     3121 tacattttca agtacgaatg gggaatactt agtactatta attataaata tcaagaataa
     3181 gatgactata aagtagtcag aaaaatatag tattcttgca attaaagcga ttactttgtc
     3241 agaactaaaa tcatataaaa acataggtat catacataag aatattatac cagcaactcc
     3301 taaaagtga
//
LOCUS       DAYAOY010000196         3250 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYAOY010000196 DAYAOY010000000
VERSION     DAYAOY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.203"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            1391..1780
                     /locus_tag="ACGI6B_003949"
     CDS             1391..1780
                     /locus_tag="ACGI6B_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753351.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2650..3120
                     /locus_tag="ACGI6B_003950"
     CDS             2650..3120
                     /locus_tag="ACGI6B_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9753352.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT         1453 a          184 c          580 g         1033 t
ORIGIN      
        1 taattataag ctgttctgtg tagttataat agcttctatg taactattgt aagtatattt
       61 tattatagtt atttatttaa agtacgataa atgttttaaa ttgaagtatt ttgcgccatt
      121 gagttctact aaagtagagt ttttgtgtag tatttttaga aataacatat atatatatgt
      181 atttatgttg aaatatatgg aaaatttata atataattaa tgaaaaaatt taaactttgt
      241 aatatataaa taaaaaaact ctaaaataga aggatataat tatatagaat gtttcaatta
      301 aacaaggtaa aaactataat cttaaaatta aaattaacat tattcttaag aagcaagaaa
      361 tttagaaagt aggaagttta gtgataagtg gtagtaagta agaaatttag gaagtgggaa
      421 gtttagttat aagtggtagt aagtaagaaa tttaggaagt gggaagttta gttataagtg
      481 gtagtaagta agaaatttag gaagtgggaa gtttagttat aagtagtagt aagtaagaaa
      541 tttaggaagt gggaagttta gtagtaataa tttatagcaa gtaagaaatt taggaagtag
      601 gaagtttagt aataatttgt agcaagtaag aaacttaggg agtaggaagt ttagtaataa
      661 tttatagcaa gtaagaaatt tagggagtag gaagtttagt aataatttgt agtaagtaag
      721 aaatttagaa agtaggaaat ttagtaataa tttatagtaa gtaagaaaat tagggagtag
      781 gaagtttagt aataatttat agtaagtaag aaatttagaa agtaggaagt ttagtaataa
      841 tttatagcaa gtaagaaatt tagggagagg aagtttagta ataatttgta gcaagtaaga
      901 aatttagaaa gtaggaagtt tagtagtaat ttatagtaag taagaaattt agtaagtgag
      961 aagtttagtt ataatttata gtaagtaaga aatttagtaa gtgagaagtt cagtaataag
     1021 ttgtagtaaa taaaaagttt aggaagtagg aagttcagta ataagttgta gtaaataaaa
     1081 aatttaggaa gtaggattta ctgaaataca aatatttaaa atcaaagagt ttaaaagaaa
     1141 ggagagctac tggacaagta tccaaaatag atagctgagt acaaaatatt tcataaaatt
     1201 aagcaatata atacaagaga attttgacat tatttatata tatttaccgt gtacaaatag
     1261 ctaaatttag gattaagatt gaaaaatatc ttataagtct gagcttatat aaatccgtat
     1321 aagatgtatt taatcgaaaa attttattat ggatattgct tgtctatatt ctgagtagca
     1381 aggttttatc atgactataa aaaagttgcc acaatcagaa ttaaagataa tgaaatttat
     1441 atggaaatca gattccaaag taacatctag agatattgtt ttaggaatgg agcaaaaata
     1501 tcaatggaaa caaacgacaa ctttgactct tttatctaga ttagtagtaa aaagatttct
     1561 aaattctcaa aagatagata aatatacgca ttatgaagtt ttaattaaag aaaaggaata
     1621 cataggagtt gagacaagag atttttttag aaatattcat gattcttcta taaaaagttt
     1681 attgttgtca ttacatgaaa atattaattt gagcaaagat gatattttac ttattgaaga
     1741 gtggataaaa aatctaaaag aagaggagaa agacgtataa gcaaacgtat ttcttatagc
     1801 atcaatataa agtgtatgta agtgtattta tgtaatatat tagtctactt aagtaatatg
     1861 acaaatgatt ataagtggat tatgatacta taatgtgaat ctaaataaat taaatataaa
     1921 ttaatagtgg atattctatt taattaaatt taaaaagcta cctttactta taagtataat
     1981 taaggtagct ttttaaatag aggtagtgta attaaagaga atttttaaat ataaattgtg
     2041 aagctttaat atagatgata atatatattt tgcaaatatg acacagtgtt ttcgtattta
     2101 agatgataaa ataaatagga attaatgatg atattgaaaa aataaaaata aaggattaat
     2161 ataagatatt aaagagatag agataaaagt gttaacagaa gatattgaaa aaataaaaga
     2221 ttgatataag atactgaaga ggtagaaaca aaagtattaa tagaagatat tgaaaaaata
     2281 aaaacaaaag attgatagaa agtactgaat agatagagat aaaagtgtta atagaagata
     2341 ttgaaaaaat aaaagattga tataagatac tgaagaggta gaaacaaaag tgttaataga
     2401 agatattgaa aacataaaaa caaaagattg atagaaagta ctgaatagat agagataaaa
     2461 gtgttaatag aagatattga aaaaataaaa gattgatata agatactgaa taggtagaaa
     2521 caaaagtgtt aatagaagat gctaaaaaag taaaaataaa ggattaataa aaggtactga
     2581 agagatagag aaaaaaatgt taatagaaga tactgaaaaa atagaaataa aaaattagga
     2641 ggaaaattta tgaaatatca ggtttcatta ttagcagtga aggatattga agtatcaaaa
     2701 aaattttata aagaattatt tgagcaagaa attgaattgg acttaggcaa aaatgtaaca
     2761 tttaaaggag gatttgctat acaagaagac ttcgcatggc ttacagggat taatccagat
     2821 acaattatac aaaaatcaaa taatatggaa ctttattttg aggttgatga ttttgacgaa
     2881 tttattgaaa aactcaatta ttataaagat gtagagtttg tccataagcc actaaaacat
     2941 gaatggcaac aaagagtagt gcgtatttat gacccggata agcatattat tgagatagga
     3001 gagtctatgg aagttatagc tagaagatat ttatctgagg gatactctgt agaagaaact
     3061 tctaaaataa ttcaacatcc aattaaattt gtaaagcagt gtgaaaatag taaggaatga
     3121 ttgtttaaat tataattatt tagtagaaat taattgtaat agaactaatt caagataata
     3181 tataggacta gtatttgaca aatgtcataa attaaattaa aataaagaaa atattaataa
     3241 agaaaaaata
//
LOCUS       DAYAOY010000197         3159 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYAOY010000197 DAYAOY010000000
VERSION     DAYAOY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.204"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     regulatory      98..191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            274..759
                     /locus_tag="ACGI6B_003951"
     CDS             274..759
                     /locus_tag="ACGI6B_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9753353.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            1115..1513
                     /locus_tag="ACGI6B_003952"
     CDS             1115..1513
                     /locus_tag="ACGI6B_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753354.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1949..2212
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2363..2914
                     /locus_tag="ACGI6B_003953"
     CDS             2363..2914
                     /locus_tag="ACGI6B_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HFG9753355.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1353 a          278 c          450 g         1078 t
ORIGIN      
        1 tattttttga ttattattaa ttaaaaattt aatttatatt taaataatat aaaatttgag
       61 gtaatattat tttttaattg ttcaaattat gttattattc agatataggc aatattagat
      121 aaatctaatg acgcaaaact atagggacta aaatctttat gtaataaagg tcatgtcagc
      181 cagttgccaa aaagataata attctttttg ttaaaagtat ttttagagtt atatatttta
      241 ataacttaat atttggcaag aggagggctt aagatgatat ctgaattaat gttaaaaaat
      301 tttcgcaaat ctagcatcaa ttttaagact aaaattaaat tgaatttaag agaaaaacca
      361 gacataaata gtttgaaatt aaaaagtata ccagaaggta aaattgttaa gttaaagtgt
      421 gtagatggaa tatgggcaga agttgagagt aattatgaca aaggttggtt actttataaa
      481 tatttagaaa gattaagtaa tgctaaaaac aatagtaatg atacaataaa aaatagaaaa
      541 aagaattaca taggaaatat aagaacaaat ggattaagtt tagaacttag aaatgataga
      601 acattagaat ctaaagttat aactacaatc cctgatggtt ttaaagttga agtatgttat
      661 agcgttggaa aatgggcaag agttaatatc aataaaaatg aaaaacgata ttctggatat
      721 gtatataatc aatacataga agagcattta agtttataat acgatactag caagctttaa
      781 atatattaat aaatcgcaaa aaagaatccc attaatggga ttcttttttg tagaaacatt
      841 ataacagaac taaagagaat aaattaacta atttattttt attataaagt aattataaat
      901 ggtaaattta attgaaagta gatttaactt atactttata caccaagtat cataaattaa
      961 tactactttg aaaaatctta aaaaaagtaa aagttatgat aataaaaata atattaaaga
     1021 taataagata tataagaaac ttgaaaaaat ttgtaatttt tagttatata tatattataa
     1081 tgaactagtt aaaaattttt atgaggggga aaatatgaat aagaagaaaa aattgattgg
     1141 gatagctata tcttttctac ttataatcag cattataaat ccattacaag tagaagcaga
     1201 agctattatt gagtctagtg tatacatttc ttcaataaaa gagaattata catatgataa
     1261 tactaaattt aaaactatta aatctaaaag tagacataaa agtaggaaat ataataaaag
     1321 taaagtaaag ttgaaaaaac gaagtataag aactaaaaag tatggatatc aaactataaa
     1381 taaatataaa agacaaaacg caagaagaca taatatccta acaaaattaa tattgtccat
     1441 aatagtcata tttttggtta tagttttagt agtgctaata tttattaaaa gaaagaagaa
     1501 aaaattttac taaaaataat taaaagaata tcattttttt aatcattggt atctatttaa
     1561 taatatgatg taattaaaat ggagtaatat tctgtttcaa aggaggcata taaatacagg
     1621 aatgagtatt aaatgatggt taattaagta aatttattga taaatgtact taattaactt
     1681 tgaagaattt aattcctcat gaatcaaaat ataaaattac atcaacagtt attcacaaaa
     1741 ttaatataag aagaattaga gccaaagagc agactattag accattaaag taattattca
     1801 tagaattttg tatatgaaaa gaaatgtata gtataattta ctttttttat aaaaaataga
     1861 ttacaaaata tattttttat aaaaaggtat tgacattttt tagaaaaaag aatatcatta
     1921 ttttaacaac aaaattcaat acaataaatt aaatcctatg acaaggaaga gtagttaaat
     1981 ttaggttcag tatagagagc tgaggatggt ggaatcttag caagaaaaaa tttgatgaat
     2041 ggcccttaga ggaataaatt gaaatcatag ttttgagagt agactttatc gtcagtcgca
     2101 cgttatagcg aataaggtat gtaagtacct gataatgaga tgtatgaaaa ttgaatttta
     2161 tattaatttt tatataattt aggtggcaac gcggataatc tccgtcctat tagtttagga
     2221 cggagttttt tattttactg aaaatttaaa agatagaagg tggtgagaaa gatgttatga
     2281 atttcaaaag ctaaagatga tgatatagta ggttggcaaa aaaataaatt gaaaattaaa
     2341 aaaataggag gaagtacaaa aaatgaaaac aaatacaaaa aaattaactt taaatgcaat
     2401 acttttagcg atgggattat taatacatca aatcacacca gctataggat taccaatgaa
     2461 gccagatgta ccactagcaa tgttatttgt aatattggtt ttaaatagag atgattataa
     2521 aacttgtttg atagcgggta tagtaacagg aatatttaca gcacttacat caagttttcc
     2581 gggaggtcaa attcctaatg taatagataa aactcttaca gcaaatatag tatttttatt
     2641 aatgaatatt agttataata tgccttttat aaaaaaatta gctaagaaaa cacaagatac
     2701 tgtagtagtt gcaataataa tgccaatagg aactcttgta agtggaacta tctttttatt
     2761 agcagctcaa gctatagttg gtcttccagg ggcttcattt acagctttat tcttagcagt
     2821 agtattacca gctgttttaa taaatctagt tgcaggaata tttttattca agattgttag
     2881 tctatcaata agaagagtta gttatcaagc ttaaacacga aataatattt tactttataa
     2941 agcattataa taactaaata acataagaat aaagtagatt ataactattt tattcttatg
     3001 ttgtttaaag atttggtatt atatatttat ttttaataaa atatttatat agtaccaatt
     3061 acacttaatt taacagataa attatcatac ctatataata ttcttatatg tatctcattt
     3121 gtagttatta aatataagat atatttaatt gacttttca
//
LOCUS       DAYAOY010000198         3152 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYAOY010000198 DAYAOY010000000
VERSION     DAYAOY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.205"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..265)
                     /locus_tag="ACGI6B_003954"
     CDS             complement(<1..265)
                     /locus_tag="ACGI6B_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753356.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIIND"
     gene            complement(259..660)
                     /locus_tag="ACGI6B_003955"
     CDS             complement(259..660)
                     /locus_tag="ACGI6B_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753357.1"
                     /translation="MENNMIDDIERRLQGFGYVLKDGDKWLIDFVREKIENIIKLDCN
                     ISEIPKELHNIEIDMIAGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNNILTFRCLRW"
     gene            complement(670..903)
                     /locus_tag="ACGI6B_003956"
     CDS             complement(670..903)
                     /locus_tag="ACGI6B_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753358.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(903..1802)
                     /locus_tag="ACGI6B_003957"
     CDS             complement(903..1802)
                     /locus_tag="ACGI6B_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFG9753359.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(1814..2377)
                     /locus_tag="ACGI6B_003958"
     CDS             complement(1814..2377)
                     /locus_tag="ACGI6B_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9753360.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITKEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(2422..2652)
                     /locus_tag="ACGI6B_003959"
     CDS             complement(2422..2652)
                     /locus_tag="ACGI6B_003959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753361.1"
                     /translation="MKEYMIWFKSGNSISGIVDEDVADKLMQDFMEADSDCKDLKVYL
                     DEDGTTVIDLSQIEAISINNCNENNNIGFSKS"
     gene            complement(2695..2892)
                     /locus_tag="ACGI6B_003960"
     CDS             complement(2695..2892)
                     /locus_tag="ACGI6B_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753362.1"
                     /translation="MFYLLLQVISICLSITVFMYVTGNIVFKIYLKSLRKEYESNIHE
                     VSDEILKRIRKELNKSLDESL"
     gene            complement(2893..>3152)
                     /locus_tag="ACGI6B_003961"
     CDS             complement(2893..>3152)
                     /locus_tag="ACGI6B_003961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753363.1"
                     /translation="NRKGEVKKTICIKYKNGVIRATNGDWIIKDSEGKIYSCTCDEFE
                     KYYKDLDNETKISNGGLECLREIALKDVNFKFELTGIKEEK"
BASE COUNT          896 a          582 c          327 g         1347 t
ORIGIN      
        1 tatcatttat aataagtttt gaacctgctt taatttctat atctggagat ataaagagtt
       61 taatagtttg ctctagctta gctactttcc cttctgtagc agaaactata tttttatatg
      121 aaagtttaca tggttgattt tctaatacaa tcacttcttt attgttagtt cgttttgtta
      181 cagggtcttt aattggctga tactcaacta tagtacattt atctctatat aacatttcta
      241 ttgcttttct agttttactt accatcttaa gcacctaaag gttaatatat tattcttacc
      301 ataagtagta agataagcta ctaagctatc aaagcgttgt tctggtgttt gagagccact
      361 tcctatagca aaatctacct ttgtatcacc ttctgatata gacttttcta cagcttcaaa
      421 gttaatactt tctatatcta attgccccat atttttcttg gtaaataaga actctccagc
      481 tatcatatct atttctatat tgtgtaactc ttttggaatt tcacttatat tacaatctag
      541 tttaataata ttttctattt tttctcttac aaaatctatt aaccatttat ctccatcctt
      601 tagaacatat ccaaatcctt gaagtcttct ttctatatca tcaatcatat tattttccac
      661 aattttcacc tactttttag taagcttatt tttctcttta agctgcttat tttcttcttc
      721 taaagactcg acttttgacc ttaaaatatt attttcagct attaaatctt ttacatttaa
      781 tgacttgcca tactttactg ccttaccagt ttcatctatc aaatcatatc ccatctctaa
      841 gaaatcatct attttacatt cttctatagt taatattcta tttaattttc ttacttgtgc
      901 cattatgctc cagctccttc aacaacaaat tgtattgcat cagctttttt atttaatata
      961 aatacatcct caaaactttc ttcaaagtag aagtattttc cttctgtaac tgctgttggt
     1021 tcgtctaact tagagaactg ataagaaaca ggtgtaatta ttgcacttgg gtgaactaag
     1081 gacataaaga tttgtttagc tcctgctcct actttccatc cagttgtaaa atcatatgca
     1141 gttttcatga ggttagatgg aactttaatt attttaactg tatcaatatc tgtggtttgt
     1201 ctatttaaag aagttccacc atcttttatg tttactgttc tttgtatctc ttttgcattt
     1261 ttgataagtg tatttactac tggagtaaca tacaatattc ttccattttc aggtactcta
     1321 gcttctgtca ttttttccat taacttatca aatacttcta atacgtttgt tgttgtaaga
     1381 acagttgtat ctgctgtgtt acctaatgcg gtccaatcag catatatttt agatatacag
     1441 taagcatcca tctctggaaa cttttgttcc tcattatata cttttgttat attgcctatt
     1501 gaagccacat aattagtttg gtttatatct gctggatgaa ccaacgttga ccatttcctt
     1561 tgattagtta ataccttagg ttcccaagca ttatcatagt ttctttgagc tactgctatt
     1621 gtatctctgt ttgaatctac tcttccagtt gtagatatag ttggtatttc tattgtttta
     1681 gaaccagtcc atctatatct tccattattt ggtgttgcat acaaatcccc gaagtttaaa
     1741 gtataaggat atgcttgtgc taaaacattt gaatattctt ttgcataatt tagtgctgcc
     1801 attttatttc ctcctattta ttattatttt catgaggtct taccccagta aaattaaaac
     1861 caaaatcatt tatcttaggc tcttgccctg gtgttatagt atctatttta ggctcttcac
     1921 cttctagtgt tgcattaaac aaataatctt tatcctgttt caaagggttt atttgctctt
     1981 caaaagcttt ttgtctatct ttactatttc ttagtgcttc tatatctaaa tgagctttta
     2041 acgctatttc atccctacat ttaatagact taaaagcatc atttaaccag taattaaagt
     2101 ccttttcttc aatttctttt ttgtaggttt cttccaaagt tttcttatca gtttcataag
     2161 ttgtttttag attctctaca tcttcttttg tcatacctcc ttcaaacttt ttaatagttt
     2221 cattagctgt attaagttgt gtttcaagat ttgtataatc ttctttagta attgtagtct
     2281 cttttatttt cttttctata gatttttgaa gagaagctac atcaattttg ttatcctcta
     2341 cttttattcc ttctagcaat tcttttaacc aatccatttt aaatatctcc tttcattttt
     2401 tacaaaataa aaaagcccta tctaggactt actaaaacca atattattat tctcattaca
     2461 attatttatt gatatagctt ctatttgtga taaatctatt actgttgttc catcttcatc
     2521 taaatatact ttcaagtcct tacaatctga gtcagcttcc ataaaatctt gcattaactt
     2581 atcagcaaca tcttcgtcta ctattccaga tatgctattg ccgcttttaa accaaatcat
     2641 atattctttc aataacagaa acctcctttt gttttctacc taataaaaaa cttcttaaag
     2701 actttcatct aatgatttat ttaattcttt tcttattctc tttaaaattt catcagaaac
     2761 ttcgtgtata ttagactcat attcttttct taaactttta agatagattt taaagactat
     2821 attacctgta acatacataa atactgtaat acttaaacat atacttatta cttgtaacaa
     2881 cagataaaac atttattttt cctccttaat tcctgtgagt tcaaatttaa aattcacatc
     2941 ttttaatgct atctctctta aacattccaa tcctccatta gaaatctttg tttcattatc
     3001 caaatcctta tagtactttt caaattcatc acatgtacaa gaataaattt taccttcact
     3061 atcttttata atccaatctc cattagttgc cctaataact ccatttttat attttataca
     3121 gatagttttc tttacttcac ctttacgatt ca
//
LOCUS       DAYAOY010000199         3146 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYAOY010000199 DAYAOY010000000
VERSION     DAYAOY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.206"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            320..586
                     /gene="rpsT"
                     /locus_tag="ACGI6B_003962"
     CDS             320..586
                     /gene="rpsT"
                     /locus_tag="ACGI6B_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFG9753364.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(773..1816)
                     /gene="holA"
                     /locus_tag="ACGI6B_003963"
     CDS             complement(773..1816)
                     /gene="holA"
                     /locus_tag="ACGI6B_003963"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFG9753365.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1770..3071)
                     /locus_tag="ACGI6B_003964"
     CDS             complement(1770..3071)
                     /locus_tag="ACGI6B_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9753366.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         1163 a          445 c          291 g         1247 t
ORIGIN      
        1 aaatgagtaa tttcttgtat tatcttataa ttattggaaa tactatcaaa atgtatgtta
       61 atagtttgta ttatctcaaa atatttgtta caaaaataaa atattaattt atatatgtca
      121 aaatatttaa aacacaaaat ataccaattt aaaaacaaga tattgcaaat tatttataca
      181 caatttacag tctaaaaaat caaaataaaa aacattatta aaatatataa ataataatcc
      241 ttgattttta gatttatttc tgttaaaatc taatatgttg acgatgaata tgataaaaaa
      301 atacagggag gtgaacacgg tggcaaatat aaaatcagct aagaaaagaa taagtgttat
      361 cgaaaagaaa actgctttaa atagagttag aaaatctcaa ataaaaactg caataagaag
      421 attcgaagat gctgttgcag ctggaaacag agaggatgct gttgctaagt tccaatacgc
      481 tcaaaaaaga atataccaag tagcttctaa aggaactata cataagaatg ctgcagctag
      541 aaaagtagct aaattagctc aaaaattaaa cggtatgaac gcttaattac cagtttatat
      601 ttcttatata taataacccc tatgtagggg ttttttattt tgtaaattta tacaataaaa
      661 aataaatagt tagacatatt atagttgatt gtatatcaaa cttaatttag cttacatcta
      721 ctctgaacta taacatatct tgctatttat tttttattaa tatatagatt ttttaaattg
      781 cttctctttt acagtatcta ctaaccaaca tttcaactgc taaagtatct cttataagac
      841 ctgtctttat tttataatca ctttctaata tatagtttaa tatttccaca attatatcat
      901 cagaaaaatt tttagtttgc tttaatgctt ttcctacaac aaattgatgc atttttaatt
      961 tatcagctat aagttttgtt gaatatccat caagttgtaa ctgtcttact tgcataatta
     1021 ttttaaattg tcttgctatc atagaaaata tacctaatac agattcgcct tcttgtatca
     1081 tatcatttaa tattttcatt gcattagaag cattttgttc tccaatataa tcgattaatt
     1141 tgaatatatc attctcaact tttttttgag ataatttatc aattacatca ttggttacat
     1201 tattcccttt accaacaaat gaacttattt tatttatttc attttctaaa tcagataatg
     1261 tcttttcact actcttatct ctgtatcctt cttgttcaat aaaatacatg atttgagaat
     1321 tatctattat aacatcattt aaagcaaaag atttttttat ccatttaaac aaatccatat
     1381 cagaaagctt atcacaatca aaaacaattc cattgtttcc tattttctta actaatgatt
     1441 ttcttttgtc tacatctcca tatacaacaa aaactatagt tgtggtgtct ggaatattgt
     1501 ctaaatgctc tattaagtat ttctcatcac tatctgtaaa attctttttc tttcctttta
     1561 atagttcaaa gtcttttatt attactatct ttctatcatc cataaatgga agtgtttcta
     1621 tagagcttat aagctgattt aagactattt ccttcccatc tattatatct aagttaaaat
     1681 caagcattcc ctcgtttaga ctatctttaa atgcttttat tgcattctca attaaataat
     1741 actctcttcc ataaaaaaga tacatttttt caaagttttt ctcttttaca ttccttataa
     1801 tatccttgta attcattctc ttgctccttt atctcttttc cttctatgta aaatatgtaa
     1861 aaaaacactc ctatgtaata aataacaaca atataaaatt taggattttc tatattaatg
     1921 tatgcaaaac ttaaattgct gattttttct agtaaaaaaa atatgctttc tataaatgta
     1981 ctattaaaaa aagatataat tttagctatt actacactta ctttaaatac gattaaactt
     2041 gcaatactta aatacattat tactcccaca aatggtacta taattaaatt tcctataatt
     2101 gaaagcaatg gtataccttt aaatgtataa taaattatag gtaaagttaa tatatttgat
     2161 gatattgtca atgataccat acttaatttt ataatactat ttattttatt atagaatatt
     2221 aatatagaaa gtgtagctaa aaaagacaat tgaaaactta cattatatat aacatagggg
     2281 ttattcataa ttaaaattat cccaattaga gacaatgccg atattccatc tttctttcta
     2341 tcaataaata ttgataaata aaaaatcata acaaatgcta tagacctaat tatggatgga
     2401 gacgcaccta ccattataca atataaagtt ataaaaatcg acagtattaa taatttatac
     2461 aatttattta tacctcttaa tagaaaggat ataattacac ataatattcc tgtatgaaga
     2521 cctgatatag atattacatg actagttcca gttctagtaa aaatttcttt ttgttcctca
     2581 gtcaaatttt caacttctgc taatagtata gaatttataa aatctgagtt tttcttatat
     2641 aagtacctat aagttttact aatataaaac tttatttccc ctattaaaga gtaaagttta
     2701 tttttatcaa taattttata gttatttata tacactatac ctttatatcc agtacttttt
     2761 atataccttc cataatcaaa atcatcatac ttcattttat ctaaactttt gaatttacct
     2821 gtaatattaa ctttctgacc aatcttcaaa tttttatttt ttgtataatc atttattaga
     2881 aaaaccccta ccttgtactg attgtatctt ttcttttcaa tttttccttt gacaactcct
     2941 tgtatagtta ttatcttatc attaagttct tcgtcgtttt ttgtgttatt tgtaatgata
     3001 aatgatatag ctaccacaaa cattaatata attaataatg gtcttcttat ttgaattata
     3061 atcactccca tataaaagta tattatttag attattttca aatagaaaat ttttaaacaa
     3121 gataaattgt tatctaaaca aaaaaa
//
LOCUS       DAYAOY010000200         3105 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYAOY010000200 DAYAOY010000000
VERSION     DAYAOY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.207"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            68..307
                     /locus_tag="ACGI6B_003965"
     CDS             68..307
                     /locus_tag="ACGI6B_003965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753367.1"
                     /translation="MKKYKIKFEEKVTLEHEIIVEIPEEISINDICHCIENKCQRVYD
                     IADYIREFNGRQIDFTEDTCGETEMLVESFRKCKE"
     gene            312..1031
                     /locus_tag="ACGI6B_003966"
     CDS             312..1031
                     /locus_tag="ACGI6B_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFG9753368.1"
                     /translation="MKYIASFSGGKDSAAMLLLILEKGLKLDDIVFIDTGLEFKEIYD
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNEKGKRKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNKLGEHKRYIGIAYDEPSRYKRLEKNCIAPLFEEKMTESDCLKYLEEKDFYYE
                     IHHRFKRTGCYLCPKQSLESLKTLRKYYPILWQKMLKFDRDSPVPFRANGVTVNDLEK
                     RFSNEDIENERQISFFSKGVV"
     gene            1034..1429
                     /locus_tag="ACGI6B_003967"
     CDS             1034..1429
                     /locus_tag="ACGI6B_003967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753369.1"
                     /translation="MDLPDNVIEIDILRINRNNDKKCKCKNREFIVDTVNKSVHCSEC
                     GFRVEPYDALYELAMNFERVNGQVNTLLKQRKEIASYKPHLIVFKRLESKYRGKKMLP
                     VCPICGEAFYFENLTSWINKDFYERRKNK"
     gene            1526..1699
                     /locus_tag="ACGI6B_003968"
     CDS             1526..1699
                     /locus_tag="ACGI6B_003968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753370.1"
                     /translation="MNKCEDCKYRAYEEDGEVYFYSDGSYEGGICREEYCSRGYEKKF
                     KNNGENCKSFKKL"
     gene            1798..2067
                     /locus_tag="ACGI6B_003969"
     CDS             1798..2067
                     /locus_tag="ACGI6B_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753371.1"
                     /translation="MITTQMYKINNEIYLNNICLEENKEITIWVEKNNTHELIWLKIA
                     NVNGKLAIFVQDIENAAIKEWQGRIAYERVISQIEDEQVEKGEID"
     gene            2090..2320
                     /locus_tag="ACGI6B_003970"
     CDS             2090..2320
                     /locus_tag="ACGI6B_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFG9753372.1"
                     /translation="MIHELKILPQYFKEVVNGNKNFEVRKNDRGFKKGDLLVLQEFDG
                     EKYTGLEIRKEITYLLDNSNYLQDGYVVLGIK"
     gene            2457..2864
                     /locus_tag="ACGI6B_003971"
     CDS             2457..2864
                     /locus_tag="ACGI6B_003971"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9753373.1"
                     /translation="MKVFLVIDGEPVGKERPRMNSITKRTYTPNKTKNYEDLIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDII"
     gene            2879..>3105
                     /locus_tag="ACGI6B_003972"
     CDS             2879..>3105
                     /locus_tag="ACGI6B_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFG9753374.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTG"
BASE COUNT         1307 a          271 c          621 g          906 t
ORIGIN      
        1 tcttttagta taaattattg ttgaagtgtt ttgtaactct caaaaacgaa ataaaggagt
       61 atttaaaatg aaaaaatata aaattaagtt tgaggaaaaa gtaactttag aacatgaaat
      121 tatagttgaa ataccagaag aaataagtat aaatgacatt tgccattgta tagagaataa
      181 atgtcaaaga gtatatgata tagcagatta tataagagaa tttaatggga gacaaataga
      241 ttttacagaa gatacttgtg gagagactga aatgttagtt gaatcattta gaaaatgcaa
      301 ggagtgattt tatgaagtat attgcaagtt ttagtggagg aaaagattcg gctgcaatgc
      361 ttcttttaat attagaaaaa ggtcttaaat tagatgacat agttttcata gatacaggac
      421 tagaatttaa agaaatttat gatataatag atgattttga gaaaagaata aactttaaaa
      481 taacaagaat taaagcagaa aaaacttttg aggaatactt ttatactgta aatgaaaaag
      541 gcaaacgcaa agggcaaata tggggttttc cgtatactct aggggcatgg tgtaatagca
      601 gattaaagct tgctcctgct aataaatatt ttaataaact aggagagcat aagaggtata
      661 ttggaattgc gtacgatgaa cctagcagat ataaaagact agaaaaaaac tgtatagcac
      721 cattgtttga agaaaaaatg actgaaagtg attgtttgaa gtatctagag gaaaaagact
      781 tttactatga gatacatcac agattcaaga gaactggttg ctatttgtgc cctaaacaaa
      841 gcttagagag cctaaaaaca ctaagaaaat actatcccat tttgtggcaa aagatgctta
      901 agtttgatag agatagccct gtacctttta gagctaatgg ggtcacagtg aatgacctag
      961 aaaagcgatt tagtaatgaa gatatagaaa atgaaagaca gataagtttc tttagtaaag
     1021 gagtggttta gaaatggatt taccagataa tgttatcgaa attgatatat taagaattaa
     1081 tagaaataat gataagaaat gtaagtgtaa gaatagagaa tttatagtag atacagtcaa
     1141 taaatctgtt cattgttcag aatgtggatt tagagtagaa ccatatgatg ccctatatga
     1201 actagcaatg aactttgaaa gagtaaatgg gcaagtaaac acattattaa aacagagaaa
     1261 agaaatagct agttataaac ctcacttgat agtttttaag aggcttgaaa gtaaatacag
     1321 gggtaaaaaa atgttaccag tatgtccgat ttgtggagaa gcattttatt ttgaaaattt
     1381 aacttcatgg attaataaag atttctatga aagaagaaaa aataaataaa ataattggtc
     1441 aaggtaagtt tgtgaatgaa actagaagtc tatagactta ctttgactta taaaagggag
     1501 tttttaaaaa ataggaggta ataaaatgaa taaatgtgaa gattgcaaat atagagctta
     1561 tgaggaagat ggagaagttt atttttactc agatggaagt tatgaaggag gtatatgtag
     1621 agaagagtat tgtagcagag gatatgaaaa aaagtttaaa aataatggag aaaactgtaa
     1681 aagttttaaa aaattgtagg accataaaaa ataaaataga gtcattggta ttgctatatt
     1741 ttattaaaaa atataaaaaa atttttgaga aaattaactt ttaaaaggag cgagattatg
     1801 attactactc aaatgtataa gataaataat gagatatatc taaataatat atgtttggaa
     1861 gaaaataagg aaattaccat atgggtagaa aagaataaca cacatgaact tatatggtta
     1921 aaaatagcga atgtaaatgg caagttagct atatttgtac aagatattga aaatgctgca
     1981 ataaaagaat ggcaaggtcg tatagcatac gaacgagtga tatcgcaaat agaagatgaa
     2041 caagttgaaa aaggggaaat agactaaaac ttttaaaggg tgtgagctta tgatacatga
     2101 attaaaaata ttacctcaat attttaaaga agttgtaaat gggaacaaaa attttgaagt
     2161 taggaaaaat gatagaggtt ttaaaaaagg cgacttgttg gtgttgcagg aatttgatgg
     2221 agaaaaatac acaggtcttg agatacgcaa agaaattact tatttacttg ataatagcaa
     2281 ttatctgcaa gatgggtatg ttgttttagg aataaaataa atgttttgtg actaggaagt
     2341 gagtttatga aacgaagaag atgcagttgg tgtggcaaat tattttatct tgaggaaaaa
     2401 tctaaggatg tttattgttg taaggaatgt agaaagaagg ctaagaaggt gaaaaaatga
     2461 aagtttttct tgtaatagat ggagagccag ttggcaaaga aagacctaga atgaactcta
     2521 taactaaaag gacctataca cctaataaaa ctaaaaatta cgaggaccta ataaaatggc
     2581 tatatcaatc taaagttaaa cattactttg aaggttatat aaaaatgact ttaagatgtt
     2641 actattctat agctaaaagt aacagtaaaa aagttaaaga gcagaaaaga aataatgtgt
     2701 taagacctag caagaaaccc gatattgata atgtggttaa gattatagct gattcactaa
     2761 atgagatagc ttataaagat gatacacaga ttgttgaggt tgtagctagt aaatactata
     2821 gtgataagcc aagggttgag gttatattag aagatattat ttaaggagga ctataaatat
     2881 gaatgagatt atgacaaatg aaaatttaag agttgtagca gatgatttag ttacagttta
     2941 tgaaactgat acaggagaga aaatagtttt tgcaagagaa ctacataata acttagaagt
     3001 taagagacag tttatagact ggattgaaga tagaattaaa caatatggat ttaaggaaaa
     3061 tgaggattat tcagtttttc acaaaaatat gaaaaactca actgg
//
LOCUS       DAYAOY010000201         3086 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAYAOY010000201 DAYAOY010000000
VERSION     DAYAOY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.208"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     repeat_region   47..273
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=114..142
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(932..3025)
                     /gene="pdcA"
                     /locus_tag="ACGI6B_003973"
     CDS             complement(932..3025)
                     /gene="pdcA"
                     /locus_tag="ACGI6B_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HFG9753375.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1076 a          506 c          293 g         1211 t
ORIGIN      
        1 gtaactagat aatcataaga taacacatta aaagaaaatt atgaaaactt acattctgta
       61 tagttaatat aaaacataag taatataaat ttatgtaaag caacaaaaga tagatttaca
      121 ttccatatag ttaatataaa accttagaga aagcgaaaac gtaccattaa aatcaaatat
      181 ttacattcca tatagttaat ataaaacgtt gtaatcatag ttccaattag ccactttaac
      241 atgcatttac attccatata gttaatataa aacaccaaaa taaacttagt attttcaaca
      301 cttacacgta tacaactctc ctaaatttgc agtgaacgag caatagtgca attgataacg
      361 cttatcatgc actctcaacg ccttatattt caactgttac gccaaatttt ttcacaaata
      421 tcgctcactg caaaattcct ataattttat tatatcataa atatattact tttgaatatt
      481 gataccaatt tataccataa taaaagcaaa aaaacgtaat ctatctacat tagatttaaa
      541 ttctataact taaagttttt tacataatat ttatgcttgt ttaatcaatc taatatatca
      601 ttcttgtata ttgcttttaa tagtcttttt tcttaaataa atatctatct gaacaagcat
      661 agtttaatta aaaacttttg tattccctta aaactgtatc tacctcttat tattttattt
      721 atgatatatt atttttaatc taaaaataga tatctatttt tattatggtt atcccttttt
      781 aaaatacttt aatcatatac tattcccaat ttaacatcca ttgaaatgct attttcttat
      841 attgcaattt ataattgata tatcttcaat ataataattt aatgtatttt tgattttaaa
      901 gcttttcagt gatattattg ataaattaga attaattatc tagctttaaa aggtcaaaga
      961 tttctgttgc tgtctgcggt tttccaaata aatatccttg tatgtaatct ggctttattt
     1021 gtttaactaa atcatactct tcttgtgttt ctacaccttc taaacatact tttattccta
     1081 catcatgaca aattgcaaca ataaaatgta taaatgtagc atcaaactta ctctttaata
     1141 tatctttaac aaatactcta tcaattttca ctatatctat tggagaaaat tttaaaactt
     1201 ctagagacga gtatccagta ccaaaatcat ccatagcaat ataaattcct aaatcttgta
     1261 aagccttaaa tttaaactgt aaaatactca tattttgtac tgtatgactt tctgtcaatt
     1321 ctaaaacaag attttgataa ggaaaatttt catcatctat aatttttact atatcttcaa
     1381 taaatgtgtc ttctaatatt tgaacagctg aaacgtttac actcacttta aaaaaaggat
     1441 agtaatcaat ccatttccta aaagtacgta atgccattcg aagcacccaa gcccctacaa
     1501 tatttatcat atcattttct tctaatattg gaataaattc caaaggagaa attgctttgc
     1561 atttgtcatt agtccacctt aaaagaactt ctacacctat tattttatga ctttctgtat
     1621 ctacttgtgg ctggaatcga agactaaacc ccctaaaatc atcattaatt gatgccttta
     1681 aatcccgcat catttctaat gaatacattt tattttttaa tatttcttgg gagaaaaaaa
     1741 caagcctgtt tttaccatgc tctttcgcat attgtaaaga gtaactagca cacttataaa
     1801 gttcttttac tgtatctcca tgttttggat atattacaca accagcagaa attgttatat
     1861 ttaatccata ttttctccat agttgaaggt gtataatcat attctgtatt tgattatata
     1921 gagttagaat ttcattttct tcaacattat caacaagaat gcccatttcg tccccatcaa
     1981 gtttataaag ttcagcatta tctggtaaaa tagattgtac agactgtgct agtgttttta
     2041 ttatattatc tccaaaatcc ctatcataca tctcattaac atttttaaaa tcgtcaatat
     2101 ctaataatac tatgcataat ttttcaatca taggatttga aattttatct tcaaatacac
     2161 tcataaattc atgataattc agtaactgtg tcaatgaatc aatcttattt tgtttaccta
     2221 attgcgtcat tattcctgca aaaatacttg gctctccaaa ttcatcacgc attagttgac
     2281 ctctacatct taaccatata tattctccac tacgattttt agccctaaat tccactgtat
     2341 gataatccat ctggttttta cctatttcag tgtttgactt ataaaaacga ttccagtctt
     2401 ctggatgtac aatttttttc cagtaaacaa gcggattttt aacaatttct cctggtaagt
     2461 caaataattc aacttgtgat ggtgaatatc gaaatacgcc tgttttcata ttgcaaatgt
     2521 atatatattc atctgtacct ctaatcaaag catcgtaaag caatgtagaa ttataatcaa
     2581 aattgtctct tgaaaaatta aaaactcctt tttcttcttt attatttgat atttcatgaa
     2641 attcattaat cttaattggt ttatttaagc ttgttgagtt tgcaatatct attgattgat
     2701 gcatatgaac aactgtgcca tcttgccatg taattaaact atcataattt tcaaaaacac
     2761 gatttaactt attacatttt tcataccact ttattaatac tcctttttta tcattcttta
     2821 ataattctaa aactttacaa aaactacaag tagaattttt ttcaggatat aatacttgcc
     2881 aacaaacttt tccttctgga tctaaaatat tatattcttc tttcattttt ttgttcataa
     2941 aaataatttc attagtatga atatttgtaa catatatatt aatctgtagt tgatttaata
     3001 taacttcaaa attatgtttg ttcatatact tatctcctcc atagttactg ctctttataa
     3061 caacattgtt aaaattattc ttttta
//
LOCUS       DAYAOY010000202         3051 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.209, whole genome shotgun sequence.
ACCESSION   DAYAOY010000202 DAYAOY010000000
VERSION     DAYAOY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.209"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..468)
                     /locus_tag="ACGI6B_003974"
     CDS             complement(<1..468)
                     /locus_tag="ACGI6B_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753376.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFN"
     gene            complement(482..1417)
                     /locus_tag="ACGI6B_003975"
     CDS             complement(482..1417)
                     /locus_tag="ACGI6B_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9753377.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(1546..2193)
                     /locus_tag="ACGI6B_003976"
     CDS             complement(1546..2193)
                     /locus_tag="ACGI6B_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9753378.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(2186..>3051)
                     /locus_tag="ACGI6B_003977"
     CDS             complement(2186..>3051)
                     /locus_tag="ACGI6B_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9753379.1"
                     /translation="YKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIEN
                     ENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGH
                     SLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSL
                     FKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIR
                     IFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRAS
                     FPKFSGGDFFE"
BASE COUNT         1032 a          436 c          346 g         1237 t
ORIGIN      
        1 attaaataat ttagaaaaat cttctgaact tattttctga gaattattta aataaaattt
       61 ttcatggtaa gtatctaata tatcttgaag caatattgca atatcatcac gagttccaag
      121 tttttctatg tttagagtat ttgatttttc atttaataaa ctactctcat agcctttagg
      181 tatagtaagc tttaattctg ccttagaatc atctttttta actgttaata tataagataa
      241 atcatttttc aaatactttg tcaaatcatt tgataatctt gtgttatctt tgtcatatat
      301 aacaatgggg gtagattctg tttttatagg gttttgaaac atgctttctg taaccatccc
      361 aagaaaccaa gataaaatca gaggcaaagc taaaaaatat attaaattaa gtataaagga
      421 gctttttaaa ctctttagca tagtttttac actcatgtat aatttcataa aatcacctcc
      481 attagtccct taaatttttt cctgttagat ttaaaaatac gtcttctagg ctaggttctt
      541 cataagaaat tttcattatt tttgaattgt ttttgtcaat gacagagagt atatctgtaa
      601 gttgtaattt tttatctata tccaattcta ataatgaatt attttctaaa acagatataa
      661 cacctttagt attttttaaa tcaaagataa gttgggcatt tatatttttt atttccataa
      721 ttaattttgt atttgtagat acgagtgatt ttaaataatc attatttcca aaagcaattt
      781 ctttaccttc atccataata aaaattttag aacaaaggtg ctcaacttct tccatataat
      841 gggaagtata tattacagta gtttcatgtt ttttagatat atcttttata aaattgaaaa
      901 tatgatttct tgattggggg tctacaccta cagttggttc atccaatatt aatattttag
      961 gatgatttaa gagtgctatt ccaatattta atctcctttg cataccacca gataattttt
     1021 tgaccttttt ctttttgact tcagtaagac ctgtaacttc taatgcttca atgattcttt
     1081 ctttaagtag ttttcccttt aaaccgtaga aagctccaaa gtactctaag ttatcaaaag
     1141 gggtaagagt ttctataatt gctaattctt gtggaactaa ccctattata gactttgctt
     1201 ttatataatc ttttgataaa tctaaaccat tgatttgaat actaccacta tttggtaaca
     1261 taagatttgt aattatattt ataagtgtag atttacctgc accatttggg ccaataagtc
     1321 caaatatctc acctttttta acagaaaagc tgatgttatc aagtactaat ttatcattaa
     1381 atctttttgt tatattattt actctgacta tatccatagt aatccctcca aaataatttt
     1441 gtataagtta aaaagctata atattataat aaagaattgt gaattataat tctattacta
     1501 taagtcacta aaaaaagtga cttaagtcac tttatatttt tagatttatt ctattacaat
     1561 tttatttttg aaagcaaaga ttgctagttg agttctgtca cgaagttcta gtttagctaa
     1621 aatattagtt atgttattct ttattgtacc ttcagataag aatagttctt gacttatttc
     1681 tttattatta agtccatttg caattagtct aattatagat atttcttttt ctgttaaatt
     1741 gtacatgtca taaagatgct cttgtttaat cgttttttca gaggtcatga ttttttcagc
     1801 tacactttta tcaagcacaa tattgccaaa aaatgaagaa cgaatccctt catgcaatac
     1861 ttcatcagag ctatctttta aaaggtatcc tgaagcacca aacttcatag catcttttat
     1921 atattcatca tcattaaaag ttgttaaaat taataatgtt atagaattat tatattcctt
     1981 tataagcctt gtacctagaa ccccatcaca tactttcatt cgtatatcca taagaactat
     2041 gtctacatca ttcattttac aaaattctaa agcttcatat ccattagaag catctcctac
     2101 aatttctaaa tcattataag atgataatat aattttaaga ccttttctaa ttaatttttc
     2161 gtcatctact ataagaactt taattttatt caaaaaaatc accccctgaa aatttaggaa
     2221 aagaagctct aattgtgaaa ccattattag aattatcaaa ttcaacttta ccatttaatt
     2281 catatattct ctcattgata ctattaagtc catttccttt ttttatattg ccacatccta
     2341 taccattatc actaatagta attataagat ttgaagtatt gaatgtaata aaaattctga
     2401 tttcagttgc ctttccatgc atcgatgagt tggaaaggga ttcctgtata agtctatata
     2461 aagctataga ttgatttctg gataagttcc aagtattttt tgaaatagtt aatttaacat
     2521 taatatttgt aagcttaata aagcttttaa ctaattcttt gattttaaat aaactttggt
     2581 aattttcata ttcaacaggt tttaactcag atatagccct tcttacatct tgaaaacttt
     2641 cagttacaaa ttctctaagt tcatgtacta agtcataaat tagaggtttg tcttttaaga
     2701 gtttttctat agcatttaat tgtattattg ttgtagaaag agaatgtcct acactatcat
     2761 gtatttctct tgatattcga tttctttctt ttaaaattgc taattcttct atagaacttg
     2821 cataagtttc caaatcagca ttaacttttt ttaattcatc tttagaaatt ctaagcttat
     2881 cataaagatt ttgactgtat aattttgata aattctcatt ttctatatat atagaaagta
     2941 cagttataat tagcaatata gagcttgact ccagtgccaa atatatattt ttatctaaac
     3001 taataataag agaagatatt ataacaaaag agaacaatat gtacttatat t
//
LOCUS       DAYAOY010000203         2915 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYAOY010000203 DAYAOY010000000
VERSION     DAYAOY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.210"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(1077..1292)
                     /locus_tag="ACGI6B_003978"
     CDS             complement(1077..1292)
                     /locus_tag="ACGI6B_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753380.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(1475..1819)
                     /locus_tag="ACGI6B_003979"
     CDS             complement(1475..1819)
                     /locus_tag="ACGI6B_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753381.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1946..2329
                     /locus_tag="ACGI6B_003980"
     CDS             1946..2329
                     /locus_tag="ACGI6B_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753382.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT         1163 a          345 c          338 g         1069 t
ORIGIN      
        1 gattagatat tacaataata gcaataatat tagtagctac tataacagta atattatatt
       61 acttagttag aaagagatat ataactatat aaaatctaat agaattaata tcaaaagaag
      121 gtagcaacta atcaccagca attaaaataa tttgtttttc aaagttaata aaagtcttct
      181 ttatagcaat gatttaaata taatcaaata ttgtaacgga gattttttta tgaataattt
      241 aagaaaacaa ttaatcaaag aaattatata agatggagaa cttataaaat ctacagatat
      301 aagtagtttt aggagtaaac acagatgact tatttcaaag agacaatagc tacaagttat
      361 cctcattctg taatacaaaa atgtttaata cattagataa gaaatacttt aaatatgttt
      421 catacaagga ctcaaaagaa ttaataagta attttaaatc tgtatatagg tcagttaatg
      481 aataagaagc cttatcgact ttagataaaa tagattaaaa ataatgtaaa cagtatactt
      541 cagaaattaa aattttggga aataactgga atgttgtagc atcatccttt agttttacta
      601 aaataagtaa tatctataat atatattaca agtataatcg aaagatttaa taaatatttc
      661 aaaaatgtct caaaaacaaa gtccagattt acatcaaacg cttcactctt aaagatatta
      721 ttcttgacaa gcgaaaatgt gatgaaaaaa tggactatga aatataaaaa ttgatataat
      781 atatacaatc atttattcat atcttttgat agtagaatat aattttaaaa attataccct
      841 aaaaatttgc tataattgtt ataatatata taatttccaa aaatatttaa aaacatccca
      901 atatacattt tttataattt attaaaataa aattcatata ttaagaaata ttaatagtaa
      961 ataagcaatt gttactataa cataggaatt atctttaaaa acacaaatta atttacattc
     1021 tctaatttta aagtatatgt atatctttga ttttaataaa attcacttaa aatattttaa
     1081 ggttgaacaa tgaaattgaa tgcccaagaa cttcctcctc ttactaatgt tgcaattaat
     1141 tcaaacccac aattgtattg aactgaatct ctacctactg gtttccaact agaaggtgca
     1201 ttatatgaga aagaatttga tgttggctct ttcttttgtt gctgcttaat tcctagctca
     1261 ttagaaataa atccagtttg atttatatgc acccttctat ttgttacacg aatctggccg
     1321 tctgcaatag accagtttcc agaaacttta ctaacggaaa tatattgaaa tgctccatct
     1381 ccttttgata ttgtatatgt aactgtacca gatgctttta caccaccagt tttatcccat
     1441 ccatcttctg atatacttgc ttttctaaga ggctttattg tattagtatc aaaaaaactg
     1501 tcttagaata tatctttgat gttaaatttt tagattgttt aacttctaat aattgagttg
     1561 ttgatataga atcaacgtat atggactctc ctgttcttgc atttttgatt ataccttttt
     1621 cattggaact tttaacatca gtaattccat ttttagccat atcgtataat ttgtccatgt
     1681 ctgttacttg tttctctgaa tacattactt tcatagatga atcttctacc ttactagcac
     1741 ttgcatttgt tggtaatagt attgtcatta tgagtaaaga taaaatagtt gttacagatt
     1801 tttttaacct ataattcata ttaatcctta ctttttattt gttataacag acattctatt
     1861 gtaatagaat ataaatccac aatattttta aaattagtat tgaataacta ttctcatgtt
     1921 ataattattc aaaggaggga ttgatatgag gaaaaaattt tttataggta ttcttatagt
     1981 tttaataatc tctgtgatag ctatacttaa tataaaaaat gataaaaatg tctttattgg
     2041 agaatcagaa aattggaaag ctaaatttgt aactactgaa ggacactcaa aattagaaat
     2101 aaaatataaa aatagtaaag atgtagttga acaaaaattt aattattcct atcaatttat
     2161 agatggtgaa gatgcagttg tagttgtaaa aaatgaaatt ttagaagcag gtcaagaaaa
     2221 agcaataaaa atacaaagac caggtcttta ttcaaataga gaatctagtt acaagctaat
     2281 tatatcttgg aatggaaatg aggaaactat aaacttgaaa aagaaataac catattatca
     2341 atttttaaaa aattattatt caaattctta taaaaagagt gtttagaaag atgaaacact
     2401 cttttttgta taagtttttg aaaactataa atgaaatata tcaaaatcgt tcgaaaaatt
     2461 actgaaataa actcttaaat atattataaa ttaaggtata ttatacatga atttattggg
     2521 agtttatgag ttttaaaatt ggaaaccaaa caataagggc gaaaaatact tcaaggaaaa
     2581 tatataaagt tctataaatt atcaaaaata tttcgcaaaa attgataatt attacaaata
     2641 aattgataat attagaaata tcgaaattga acaaactagg attaaagctc aattagaaaa
     2701 atccactaat cctgaaaata ctttgttatt ctcatttatt actgttgttt tttcattatt
     2761 tgctaattta tttttcagtt cattaaatgt taaatataac tctataaact taattttaca
     2821 gttaatatgt attatagttc ttatatttat atatattaat acatttttat atcgcctctt
     2881 caatttgagc cccatcatta tataaaatta accta
//
LOCUS       DAYAOY010000204         2895 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYAOY010000204 DAYAOY010000000
VERSION     DAYAOY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.211"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            543..1364
                     /locus_tag="ACGI6B_003981"
     CDS             543..1364
                     /locus_tag="ACGI6B_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9753383.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(1674..2453)
                     /locus_tag="ACGI6B_003982"
     CDS             complement(1674..2453)
                     /locus_tag="ACGI6B_003982"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFG9753384.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         1076 a          383 c          344 g         1092 t
ORIGIN      
        1 gactagaatt tgattaggat attatcttaa tttgtcagat aaatcaatta taagattata
       61 tactatcctt atactctagt taaatatatt ttaatcacta atataaatta gtttttttaa
      121 caaaacaata aaagagtaaa gtacaaataa ttcctttgta ctctactctt tttaactttc
      181 tttgtacaat aatacaatga taaatataaa aaattatata ttccattcta tagaaatatg
      241 ctcaccaata acatgtatgt tgttaatcaa tatattatat caaattatat caaaattaca
      301 taaaccttat aggacaatca tatcttatta caagaaaaat gtcttatcca tattgaataa
      361 gtcattcagt tcatatacaa agtcaatttc attgagaaac atttaatata tgtttataaa
      421 aatttcatac tattctttca tatttacatg tgatataatt tattaactca taattttgtt
      481 aatcagttgt gcttgtatat tatttatata atctaatatt tttagaaggt ggtgtaataa
      541 ctttgaaatt aaaaattgtt ggtagtggcg gtatgtcaat tattccaagt tcattttgta
      601 aatgcaaaat atgtgaagaa gccaggcaaa aaggcggaag atatgaacga ttaggaccaa
      661 gtttatatat tgatgatata aaaatgctga tagacacacc tgaagacatt gcagtagctt
      721 gtaacagaca aaaaatatcg gaagtaaaac atatttcaat ttctcaccat gacccagacc
      781 atgttaaggg tattagaatt gtcgaaaaaa taggatgtga ctttatcaca ggagaaaata
      841 atccaattgg cttttatgcg cttcctgaag taattgaaga tataaaccga acgaatctcg
      901 attgtcttaa atattatggt gatgttataa attgcatttc tattaatgaa acttcgcatg
      961 taaagataaa taattttgat attgatttga ttaacaataa cccagacagg aatataacct
     1021 tctatgttat caaagagaat gaaaaaaaag tcatatatgc ctgctgtaat cccaagccat
     1081 ttactcatag tgatatgtac tttgatgctg atattttaat aataagtcta gtttctgatg
     1141 atggtatatt aaatgatggt acaaaactta aagacacacc atttaaagat gaaatattta
     1201 ctttagatga gattgttgaa attaaaaact attatagaat taaaaaaatt attgttacac
     1261 atattgacga aatgtgggga aaatcatatg gttattacag tgaattagaa aagaaacttg
     1321 ataacattta ttttgcatat gatggtatgg aaattatagt ctaaatcatt aagtaataag
     1381 cataatgcaa aataagaaaa cttcaattta acagtatagt caaactatgt gatatcaaca
     1441 tatcgcagtt tttacaaaat atcatagtat ttatctttat aaaatttcaa gtcctacata
     1501 ttttaatact tcaatatcta aataaagtaa aatcaaaaat atattttata tacattatag
     1561 acggcttatc gtcactagat aagccgtttt gtcacaatta ttatttgctc aacaaaactt
     1621 ctcataatat ataacagatt gaatctccaa ctgatactta attaccaaaa ctcttattcc
     1681 ttaatatgtt gttgcattaa ccaaaccttc atcatcattt tttgtggcaa tagttttgct
     1741 aataaatgac tggattttgt atttatacca tacacagaaa tatccttatt tttctttgca
     1801 tccttcaatg cttttttggc aacaacgtct ggagacacta tgcctgggaa attttttgtc
     1861 cctttttcag ctacaactat acctctctta aatagatttg tactcatcca acctggacat
     1921 acagcaattg catttatccc tttactctta agttcaacat ttaatgctct tgtataattt
     1981 cttataaacg ctttagttga actataaata ttttgatatg gcaatggctg gaatgatgct
     2041 tgtgaagaaa tattcatgat atggcttccc ttttgcatat atggaataca tataagtccc
     2101 aatgctacaa cagcactaat atttaaatca atcatattaa tagattcatc tatacttaaa
     2161 tcattgtatg aacagaattt tgcaaaacca gcattgttta taaggtattt tatacataca
     2221 ttttctttac ttaaaaatgt accaatttct tttacttctt ctatttttga taaatctttt
     2281 gaaaaaattt ttaccttact tccaaattca tctactaatt gatttaatct cgccttgttt
     2341 cttgcaactg cccatatttc tgttatttct tcttcattta ttaataactt tagaaactct
     2401 ttaccaaaac cactattagc acccgttata attgcaatct tcttataact cataattatt
     2461 ctcctttacc ataagtttat acgctcaaac tgtaaactta ttttttacaa ataaaacacc
     2521 ctctattcag atgttatatt catattattt gagatattat atttatatca ttttcaataa
     2581 ttgttatata gcataaaaaa tagtgttaat attatagaat cggtattgat tatatttaaa
     2641 aagtagtgtt aattatatta tagaatcagt atttattata ttataaaaag tagtgttaat
     2701 attatagaat cgatattgat tatatttaaa aagtagtgtt aattatattg tagaatcagt
     2761 attgattata ttgtaaaaag tagtattaat aatattgtag aatcaatatt gattatattt
     2821 aaaaaagtag tgttaattat attgtagaat cagtattgat tatattataa aaagtagtat
     2881 taataatatt gtaga
//
LOCUS       DAYAOY010000205         2650 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYAOY010000205 DAYAOY010000000
VERSION     DAYAOY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.212"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..406)
                     /locus_tag="ACGI6B_003983"
     CDS             complement(<1..406)
                     /locus_tag="ACGI6B_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9753385.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDV"
     gene            594..1499
                     /locus_tag="ACGI6B_003984"
     CDS             594..1499
                     /locus_tag="ACGI6B_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HFG9753386.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1508..>2650
                     /gene="eis"
                     /locus_tag="ACGI6B_003985"
     CDS             1508..>2650
                     /gene="eis"
                     /locus_tag="ACGI6B_003985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced intracellular survival protein Eis"
                     /protein_id="HFG9753387.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNC"
BASE COUNT         1056 a          239 c          438 g          917 t
ORIGIN      
        1 aaacatcatt tttatagaga tgctgaatag ataagttttt atccgtacaa gaatttatta
       61 gattttgata agcctcacta tcagttgatt gttcaggcat atatatggag tttacattaa
      121 atttttttat aatattatca agacttccta tgtggtctga atctggatga gttgctatta
      181 taatatcaat agtttttatt cttttttgtc taagataact aataattata ttttcgctat
      241 cttcatcacc tccatcaatt aatatattct tatttgtggg tgtttgaact aaaatactat
      301 caccttgtcc aacatcaatc atatgtatag atagtagaga ttttttgtca cagccgatta
      361 aaagtgatat tataataagt attgataaaa aaattttatt tctcaataaa aaatcactcc
      421 attcattaca tgtttatata aatttatgat gggtaatata agatatatat gacttattat
      481 attttatatt ttaaacaata taaatgaaat taataccata ataaaattgg agttttttat
      541 atatacaaaa taaaattttt aaaattaagc aataaaaaag gtaggaggat tttatgtttt
      601 tcaaagatat tgaactaaat tcaaaaaaag agttagaccc atattttgat ttagtagatt
      661 atgaagcttg tgagtactgt tttagtactt tatatatgtg gcaacatgtt tataagacag
      721 gatattatat aggtgaagat tttgccgttt tagttgggga atacgaagga gatagcttct
      781 caatattacc acttgctaaa aaagataaac ttccagaagt tgtagatttt gtccttgaat
      841 atttttctaa aaataataaa aaaatatact taagaggtat tactactgaa gtagtagagt
      901 ttttaaaaga aaaatatcca ggtagatttg agtatataga agaaagagat ttatttgact
      961 atatttatga tgcagaaagt ttaagaactc tagctggtaa aaagaatcaa aagaagagaa
     1021 accatataaa ctatttctta aaagagtatg caggtagata tgaagctaaa ttattagata
     1081 aagaaaactt tgatgagtgt ttagtactaa tgaaggaatg ggaaagtaat aaggaagaaa
     1141 ataatgaatt tgatgaaagt atggatgatg aattaatagg tatcaaaaag atatttaacc
     1201 attatgacat attaaaagat aaggtaaaag tgtttggtgt ttatgtagat ggaaaattag
     1261 aagcttttag tataggagag cttttaaatc caaatatggc attgattcat atagaaaaag
     1321 ctaatcctga tataagaggt ctgtatccat ttataaatca acaatttttg gtaagtgaat
     1381 ttaaggacgt tgagtttgta aatagagaag aagatttagg tattgaagga ttaagaaaag
     1441 ctaaactttc ttatcatcct tgcagatttg ttgaaaagta cagtgttagg gaggcttaat
     1501 agactatatg gagattaggt atgctaaaga agaagagata gaaagtataa aagagatttg
     1561 gagttattgt tttaacgata cggagagttt tatgaagtac tattttaatg ataaatataa
     1621 atctgaaaat actgtagtag ctttagatga aggaaaaatt atttcttctc ttcagctaaa
     1681 tcagtataag ttattactaa atagcaaggt atataataca tcatatgtag taggagtttc
     1741 tacacttcca gagggaagag gagctggata tatgaacaaa gtaatgaaat ttactttaaa
     1801 cgaattgtat aaaaaagggc aattagtttc tatattgatg cctattgact atagacttta
     1861 tagaagattt ggatatgagc attgttacga ccagattgaa tatactatta acacagatga
     1921 tttaaaaaat tttaagtcaa gtggaaaaat gataaagtct aatttatcac aaattgatga
     1981 tttgatacaa atagatagaa cttttttaaa tgaggttaat ggtaatgtgt taaaagatga
     2041 acattattat gaaaacttat ttaaagagat tcaaagtgaa gatggatttt tatacattca
     2101 tgaaggaaat gaaaaagatg gatatatagt ttattttctt caggaagata aaatgtttgt
     2161 cagagagtta ttttataaaa atatagatgc ccttaaatca atgcttaaat ttatatataa
     2221 tcacaatact caatgtaaga tagttactat atctactcct acaatagata agataagatt
     2281 tatattagac aaccctaaag atagtgatat aaaaataaaa ccttttatga tgggaagagt
     2341 tataaatgta aagaagttta ttgaagatat agatattgag aatgatatta atagctcatt
     2401 taatttatta atagaagaca agtttataga tgaaaataat ggtctattta aaatttctat
     2461 acagaataag aaggtatctg tagagcagtt agataaaaaa ggtgcagaaa agcctcaaga
     2521 agattttgat attaaattag atataaatac cttaacacag ttatcatttt cttatataga
     2581 tgtaaatgaa gctattttct taaatgatat aaaagatgtt tcagaagaaa cactagaaac
     2641 acttaattgt
//
LOCUS       DAYAOY010000206         2630 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYAOY010000206 DAYAOY010000000
VERSION     DAYAOY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.213"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            128..2407
                     /locus_tag="ACGI6B_003986"
                     /pseudo
     CDS             128..2407
                     /locus_tag="ACGI6B_003986"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         1062 a          258 c          428 g          882 t
ORIGIN      
        1 aataaagttt ataattttgt ataattacag taaatatgga aaaataagta ttattataaa
       61 atgtttacaa taattgtcat aagataatga ttatacatac atagattcga gaaaggcagg
      121 aagcataatg ttaagtgatg caaaaaataa taggtatggt ttgtccataa ttgctgtcat
      181 aagtatctta gtaattacaa tttcctgttt gatttatggt tcaaggttga ataagacatt
      241 aggtgaagaa acgaatcaat atctttcaga aatagcaaat caaagcgtca atgtgttaaa
      301 aaaacaaatt aatggagata taaagttact agagtcaatt tcaatattta ttgaaggaga
      361 agagtctttt gaagtagata atatcctttc tatactaaaa agacaagcta ttaatagttc
      421 atttaaaaga atgggagtta ttttaccaaa tggtacagca tacactacag atggttatat
      481 agaagacttc tctaaaagag attatttctt aaaatcaatg caaggagaag tggttataac
      541 aggaaaatta aaagatgtaa tagaagatga taaaaatacc aatagtaata ttaatgtata
      601 ttctgtacca atttataaag ataatgaggt taaaggagtt atatttgcca cacatagtac
      661 gaagttgtat gaaaaaatac tttctgtacc tacatttaat ggaaaaggat attcttatat
      721 aactagaagt aatggtgata ttgttttaaa tccaagcagt aaaaacgcca actctactaa
      781 agaaaattta ttttcttaca ttggcagaag tgattcaata tcaaaatcta gtatcaacaa
      841 tatgaagtca aatataaaaa actttaaatc aggttcatta agttatagta tgtttggaaa
      901 aggatattat ttaagttatg ccccaatagg aatcaatgat tggtatcttt tttctgaagt
      961 tcctaaaact gctatatctg agaaatcata tgctattata aaacttacat tatcagcatg
     1021 tgtagtcttg attataatct ttacggcatt aacaatttat atactattta tacataaaaa
     1081 gagtaaggaa aaattggaag aatttgcttt taaagataac gttacaggta ttggcaattc
     1141 aaataaattt aatcttgaag ggggaaaatt tttatcctct catgagaaaa agaatctagt
     1201 tttaatttat tttgatatag ataagtttaa gctagtaaat gataggtttg ggtatgaaga
     1261 aggagataga gtattaaagg aaatagcaga gataattaag aatatattta aagagcaatc
     1321 tgtattttca agaatttcaa atgacaattt tgctataata tttgaaaaga aaaataatag
     1381 agagtcaatt atagaaatat gtgaacttat acgtaaaaaa ttgtcaatga taaaaacaag
     1441 tttgggagta gaactcaatt taattccatc cataggtgta tattttgttg aagagggaga
     1501 aacaaatatt tctacctgtc ttgacaaagc tatgattgca aaaactacag taaaaagaaa
     1561 atatttaaaa gtttatgaga tttatgctga aaatttaaaa gaaactctca tagaagaacg
     1621 tgatattgaa caagaaatgc atgatgcttt aaaaaatgaa caatttaaag tttatttaca
     1681 gcctaaggtt gatttatcaa ctactaaaat tgtaggtgct gaagcacttg tacgttggca
     1741 acatcccaaa aaaggtctaa taagtccagg tgtatttatt cctatttttg agaaaaatgg
     1801 ttttataaca gaattggata tgtttgtatt tacccaagtt tgtaaaaatt ttaaacgttg
     1861 ggaaaatgaa aatttcccca catttcctat atcaataaac ttatcaagag tacatcttga
     1921 gaatccaggc tttatttctg agcttgaaag aataacaaaa gaatatgaaa ttgaaccaaa
     1981 tttaattgag attgaattga cagaaagtgc aatatttgac aatacaaaaa tattatttaa
     2041 aataatgtaa actttgaaat ctgttggatt taaaatttct atggatgatt ttggttctgg
     2101 ttattcttct ttaaatatgc ttaaagatat gccaattgat gtattgaaat tagataggca
     2161 attctttata acagtgggaa atgcaaaaaa gagtcaaatt gtagtttcaa gtattgttca
     2221 aatggcaaag caacttgata taaaagttgt ttcagaaggt gttgaaactg tagagcaagc
     2281 agagttttta aggtttatag gttgtgatat ggcccaagga tttttatttg ctagaccaat
     2341 gccaatagaa gaatatgaaa aattaatatt agatagatat agtttaaata gaaaagaaac
     2401 aaattaaata aaattgtgaa ttataacata aaaatttata aaaaagtgaa ctttgttaat
     2461 caatgttcac ttttttatgt aaaaatatac tattataaaa acataaatca ttgttaggat
     2521 attattttag gaattattgg gcaaagttta ttaatatgta gtatttttta catttgaatg
     2581 tttttgaggt aagagtatat gggattatag caaaatactt tagatagtaa
//
LOCUS       DAYAOY010000207         2602 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYAOY010000207 DAYAOY010000000
VERSION     DAYAOY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.214"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..837
                     /locus_tag="ACGI6B_003987"
     CDS             <1..837
                     /locus_tag="ACGI6B_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFG9753388.1"
                     /translation="VLNKTCLEDVKLSWQAKGLHSYLISKPDHWKIYVNDLYKRSKNG
                     RDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPLENPQKLKSRKTDFPE
                     TGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTTYIKKYFEKYIGVITP
                     NNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNNWIEAGVKTNLELTEY
                     QNEFERKKKNKQDKKQSNSKAVNTPNVSKNKFHNFNETFTQYSPDELDDIIKKSQKVK
                     FK"
     gene            898..1071
                     /locus_tag="ACGI6B_003988"
     CDS             898..1071
                     /locus_tag="ACGI6B_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFG9753389.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            1087..1494
                     /locus_tag="ACGI6B_003989"
     CDS             1087..1494
                     /locus_tag="ACGI6B_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9753390.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            1564..1725
                     /locus_tag="ACGI6B_003990"
     CDS             1564..1725
                     /locus_tag="ACGI6B_003990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753391.1"
                     /translation="MSRTAICSVCGEFLVESYDDQFTNTKVMDLHGREFVIVICKSCL
                     EELLRKCED"
     gene            1730..1951
                     /locus_tag="ACGI6B_003991"
     CDS             1730..1951
                     /locus_tag="ACGI6B_003991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753392.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQHKKYKELWIDDIEGVEFTVE
                     KTFDDGYVCYPNEFWFNFGVVSEWCVEVK"
     gene            1969..2349
                     /locus_tag="ACGI6B_003992"
     CDS             1969..2349
                     /locus_tag="ACGI6B_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HFG9753393.1"
                     /translation="MAKIWLDAGTFLEKTIDIEDMFELNLRKVRKANESKKIKLKLNE
                     SKFKKIRKKPTNDTKSKPIKVFNIETGEVRIFKSAKAASKYLKISGDYASCLARENRV
                     TREGWKAEYIQEVSDGISKCGASN"
     gene            2318..2467
                     /locus_tag="ACGI6B_003993"
     CDS             2318..2467
                     /locus_tag="ACGI6B_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753394.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMI
                     RNESN"
BASE COUNT         1070 a          249 c          528 g          755 t
ORIGIN      
        1 gttttaaata aaacttgttt ggaagatgtg aaattaagct ggcaagcaaa aggtttacat
       61 tcatatctga ttagtaagcc cgaccactgg aaaatctatg ttaatgattt atataaaaga
      121 agtaaaaatg ggagggatgc tacagcaaat attttaaggg agctcataga aaatggatat
      181 ataacaagaa caccttgtcg agattctaat actaataaga tgcttggagg atatgattat
      241 caagtatatg agataccact tgaaaatcct cagaagctaa aatcccgaaa aactgatttc
      301 ccggaaaccg gatttcccgg aaaccgggtt tctcggaaac cggaaaacac ggaagtagta
      361 agtaatgact ttaaagtaaa taatgatatt actactattg ttattaatga acaatccaat
      421 aaagacaaaa ccacctacat aaaaaaatac tttgaaaaat atataggtgt gattactcct
      481 aataacttta tagagttaat gagttactta gatgatggaa tggaagctga tgtaattata
      541 agagctattg atgaagcaat agcaaatgga gttaagaatt ataagtatgt aaagacaata
      601 ttaaataatt ggatagaagc aggtgtaaaa actaatttag aacttacaga gtatcaaaat
      661 gagtttgaga ggaagaaaaa gaataaacag gataagaagc agtctaatag taaagctgtg
      721 aatactccta atgtgagtaa aaataagttt cataacttca atgaaacatt tactcaatat
      781 tcacctgacg aactagatga cataattaag aaaagccaaa aggttaaatt taaataaaat
      841 taaacttcta ggaagtaaat atcaatatat tgcttcctag aaaaggggag gtataaaatg
      901 gcgagaatat atgcacaaag aagtggttct ttaaatgaac aagatagatt ggagttatta
      961 agattacttg ggaaagctgg atatacagta aaggttgcta gagagaagca aaatagcaag
     1021 acaacttata cttactttgt tgagtataca gaagaacagg aagaaaaata gaagggggct
     1081 agttaaatga atacaataac tttagttgga agattagttg cagatgcaga attgaagtac
     1141 cttccaaatt caggtactcc aaaaataacc ttttcaatgg cagtagatag aaggtttaaa
     1201 gataaaaatg gaaataaaat aactgatttt attcaatgcg agcaattagg aaaacatgta
     1261 gagaatttag tgcaatatct tgttaaaggt aagcctatat atgctgttgg agagttaaat
     1321 atatataatt acaaagatga aaatggttgc tggaaatcta ttactaaggt taatgtaaat
     1381 gctttagaac tactttctag taaaaatgat aataatgcta aacaagaata tgtaccacca
     1441 ggattagacc cacaaggttt tcaagcaata gatgatgacg atataccttt ttaattaagt
     1501 taaatagtct agggagtaat tatacaatat tacttcctaa aagttaaaaa tggagggata
     1561 aaaatgagta gaacagctat atgtagtgtt tgtggggagt ttctagttga gagttatgat
     1621 gaccagttta caaatacaaa ggttatggat ttacatggta gagaatttgt aattgttata
     1681 tgtaagagtt gcttagaaga attattaaga aaatgtgagg actaacaata tgagatttga
     1741 gataggtaaa acttataagt ttgataaaga gaaatttatg gaaattaatg gtgtagaaca
     1801 acataaaaaa tataaagaac tttggattga tgatattgaa ggtgttgaat ttactgttga
     1861 aaaaactttt gatgatggct atgtttgcta tccaaatgaa ttttggttta attttggtgt
     1921 agtttcggaa tggtgtgttg aagttaaata aggtaggagg aattagatat ggctaaaatt
     1981 tggttagatg caggaacgtt tttagaaaaa actattgata tagaagatat gtttgagctt
     2041 aatttaagaa aagtaagaaa agcaaatgaa agtaagaaaa taaagctgaa acttaatgaa
     2101 tcgaaattca aaaaaataag gaaaaaacca actaatgata caaaaagtaa acctataaaa
     2161 gtttttaata ttgaaactgg agaagttaga atatttaaaa gtgcaaaggc tgcaagtaaa
     2221 tacttaaaga ttagtggaga ttatgctagt tgtttagcta gagaaaatag agtaactaga
     2281 gaaggttgga aggcagaata tattcaagag gtgtcagatg gtattagcaa atgtggagca
     2341 agtaattaag ttagctgaaa agatattaaa taagaaaaag tgttctgtta ataaagctat
     2401 tgatatagct ataaaaatat taagcaaata tgagtgcgag gggatgatta gaaatgagtc
     2461 taattaagta cagaggttat gattttgaga atgagaagtg gatttattct gaaacaataa
     2521 agtggagtga tgcagtagat tgtttattta tgctaaaaga gaattgtgaa tggcaaaaag
     2581 tatctaatgt tggagtattc tc
//
LOCUS       DAYAOY010000208         2576 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYAOY010000208 DAYAOY010000000
VERSION     DAYAOY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.215"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            68..1141
                     /locus_tag="ACGI6B_003994"
     CDS             68..1141
                     /locus_tag="ACGI6B_003994"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFG9753395.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKITINARYLKTKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVIYEIAKRMG"
     gene            1223..1453
                     /locus_tag="ACGI6B_003995"
     CDS             1223..1453
                     /locus_tag="ACGI6B_003995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753396.1"
                     /translation="MQKYKIKFEEKVTLEHEVIVEIPEEISINDICHCIEQKCQRIYD
                     IADYIREFNGRQIDFTEDTCGGTEMLVESFRK"
     gene            1708..1893
                     /locus_tag="ACGI6B_003996"
     CDS             1708..1893
                     /locus_tag="ACGI6B_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753397.1"
                     /translation="MVITEFKVTWQTTREWEKGNVYSSIFTNLEEARAFKKDEENIKE
                     NYNVKLLSRAIVEIEID"
     gene            1907..2146
                     /locus_tag="ACGI6B_003997"
     CDS             1907..2146
                     /locus_tag="ACGI6B_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753398.1"
                     /translation="MNTLIYKYIVNEHRDEADELDRDYGEFKIFNGGKCFGGCGRNFY
                     TLAKFKKMIVTCPHCGKKYQIKLFKNGKVNMRVIE"
     gene            2275..>2576
                     /locus_tag="ACGI6B_003998"
     CDS             2275..>2576
                     /locus_tag="ACGI6B_003998"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9753399.1"
                     /translation="MKVFLVIDGEPVGKERPRMNSITKRIYTPNKTRDYEELIKWLYQ
                     SKVKYRFTGYIKMTLRCYYSIAKNNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSL"
BASE COUNT         1040 a          251 c          547 g          738 t
ORIGIN      
        1 tcttttagta taaattattg ttgaagtgtt ttgtgactct caaaaatgaa aataaggagg
       61 cgttgtattg cttacatttt tagatttatt cgcagggata ggtggtttta ggctaggtat
      121 ggaaaaagca ggacataaat gtttgggaca ttgcgaatat gataaattcg caaatttaag
      181 ttataatgcc atgcacaaac cgaaggagga tgaatggttt gaaagagata ttagagaaat
      241 tagaacagaa aatatcccaa gagcagatgt ctggtgtttt ggattcccat gtcaagacat
      301 ttctgttgca gggaaacaat ttggattcag aggagaacgt tcaagtttat tttttacagt
      361 tacaaaactt attagagaac tcaaagaaga agatagaccc aagtatttac ttattgaaaa
      421 cgttaaaaat ctacttagtg ttaatggagg atttgatttc ctcaaagttc tcgttgaact
      481 ggatgaaatc ggctatgatg cagagtggca agttcttaat tctaaaaact tcggagtacc
      541 ccaaaataga gaacgaatat tcattgttgg acattttaga ggacgaagta cacgaaaagt
      601 atttcctatc gaaagaaaaa gtggaaaaaa tcttgagcaa ctaaataatc caactcatag
      661 tacaaataga atttatgatg cagttggaat tgctagatgt attagaagtc aggcaggagg
      721 tggaggtgct aaaacaggtc tatactttat agacttaaat aaaaactcta aaataacaat
      781 aaatgctaga tatcttaaaa caaaatataa tgcaggtgtg acaaatagaa attgtgataa
      841 tagtggagtt ttagttaatg cagttttaac gcccgatagg gtaaataaaa gacaaaatgg
      901 tcgtagaatt aaagaaagcg gagaaccaat gttcacattg acagctcaag ataaacatgg
      961 aattttgaaa aatggagata taagaaggtt aacaccaaag gaatgcttta ggttgcaagg
     1021 atttccggat aaatattacg aaagagcagc aagtgtatgc tcagatagtc aactgtacaa
     1081 acaagcggga aatgctgtta ctgcaaatgt tatatatgaa atagcaaaaa gaatgggcta
     1141 aaagttgcaa aatgtctttt agtataaatt attgttgaag tgttttgtaa ctctcaaaaa
     1201 ctaaaataaa gggggaatta aattgcaaaa atataaaatt aaatttgagg aaaaagtgac
     1261 tttagaacat gaagttatag ttgaaatacc agaagaaata agtataaatg acatttgcca
     1321 ttgtatagaa caaaagtgtc aaagaatata tgatatagca gattatataa gagaatttaa
     1381 tgggagacaa atagatttta cagaagatac ttgtggaggg actgaaatgt tagttgaatc
     1441 atttagaaaa tgaaagtagt gagcatatga ctaataaaga tatgtgcaag tcaaagaatc
     1501 ttgatgaaag agaagtgtat aagagttttg ggaaagagat ttgtaaatga taaaggagat
     1561 tgtgaaagta aagattgtga tgcaacatat gaaaattggt tggagaagga tgctgaaaga
     1621 taattataaa aataaagtca aggtaagctt gtgaatgaaa ctagaagtct atagacttac
     1681 tttgacttat aaaaaggagt ggtcaatatg gttattacag aatttaaggt gacttggcag
     1741 acaactagag aatgggaaaa agggaacgtg tacagctcta tatttactaa tttagaagaa
     1801 gcaagagctt ttaaaaaaga tgaggaaaat ataaaagaaa attataatgt gaagctttta
     1861 agtagagcta tagtcgaaat cgaaatagat taggtaggtg tttttaatga atactctgat
     1921 atataaatat atagttaatg aacatagaga tgaagcagat gagttagata gagactatgg
     1981 agaatttaaa atatttaatg gaggtaaatg ttttggtgga tgtggtagga atttctatac
     2041 attagctaag tttaaaaaaa tgatagttac ttgtccccat tgtggaaaaa aatatcaaat
     2101 aaaacttttt aaaaatggaa aagtgaatat gagagtaatt gagtaaatca agggaagtga
     2161 ttatatgaaa agaagaagat gcagttggtg tggcaaacta ttttatcttg atgaaaaatc
     2221 taaggagatt tattgttgta aggaatgtag gaagaaggct aagaaggtga aaaaatgaaa
     2281 gtttttcttg taatagatgg agagccagtt ggcaaagaaa gacctagaat gaactctata
     2341 actaaaagga tctatacacc taataaaact agagattatg aggagttaat aaaatggctg
     2401 tatcaatcta aagtgaagta tcgttttact ggttatataa aaatgacttt aagatgttat
     2461 tactctatag ctaaaaataa cagtaaaaag gttaaggagc agaaaagaaa taatgtgtta
     2521 agacctagta aaaaacccga tattgacaat gttgttaaga ttatagctga ttcact
//
LOCUS       DAYAOY010000209         2497 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYAOY010000209 DAYAOY010000000
VERSION     DAYAOY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.216"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(155..2200)
                     /gene="bclA1"
                     /locus_tag="ACGI6B_003999"
     CDS             complement(155..2200)
                     /gene="bclA1"
                     /locus_tag="ACGI6B_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HFG9753400.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          573 a          560 c          380 g          984 t
ORIGIN      
        1 ttaaataaaa atccataaat ttaaaatttt tatgaatata ttttattatt attgttattt
       61 tattcattct aaaataggta aaactcaata tataaaaata cttttgtcat ttaaataaag
      121 cctaaatagt ttttaagatt attttagaca cgttttaagt cataacagta tcagctattc
      181 taaatatcgt caaagtagcc gcagttcctg ctgattgccc atttcgtatc cctactgttg
      241 tagatgaaaa attatttatt gtaaaagtag tcccaccaaa taaaaaacca gcaaaatatc
      301 cagaaagtac tcctccgcct cctggctcag tagtaagccc tgccaaatct tttcctccta
      361 aaattgggaa taggatagcg aaagaatatt gatttatact tgatgaacct ggttgtaatg
      421 ctactgaaac tgatatataa tacacaccat tttcttgtac tgtaaacgta ccatttgcat
      481 ctactgttat acttgaacta tttcctatta ttgtttgcca acctgttata gcatctccat
      541 tatttagtgt ttgtggagat ggtgcattta cgaggaagtt agcaactggc tgagaggaat
      601 tgcctgttgc tcctgttgga cctgttgggc ctgttactcc tgtcgctcct gttgctcccg
      661 ttggtcctat acttcctgtc gctcctgttg gacctgttac tcccgttggc cctatacttc
      721 ctgtcgctcc tgtatttcct gttggcccta tttctcctgt cactcctgtt gggcctgttg
      781 ctcccgttgg tcctatttct cctgtcactc ctgtatttcc tgtcggaccc gttgctcccg
      841 ttggtcctat ttctcctgtt actcctgtat ttcctgttgg acctgttgct cccgttggtc
      901 ctatttctcc tgtcgctcct gttgggcctg ttgctcccgt tggtcctata cttcctgtca
      961 ctcctgttgg gcctgttgct cctgttggtc ctatttctcc tgttggtcct atttctcctg
     1021 tcgctcccgt tggtcctata cttcctgtca ctcctgttgg gcctgttgct cccgttggtc
     1081 ctatttctcc tgttggccct atttctcctg tcactcctgt cgctcctgtt ggacctgttg
     1141 ctcctgttgg tcctatactt cctgtcactc ctgtcgctcc tgttgctccc gttggtccta
     1201 tttctcctgt tggtcctgtt gctcctgttg gtcctatact tcctgtcact cctgttggac
     1261 ctgttgctcc cgttggtcct atttctcctg tatttcctgt tgggcctgtt actcctgttg
     1321 gtcctatact tcctgtcact cccgtatttc ctgtcgggcc tgttgctccc gttggtccta
     1381 tacttcctgt cactcccgta tttcctgtcg gacctgttac tcctgttggt cctatacttc
     1441 ctgtcgctcc tgtacttccg gttgggcctg ttactcccgt tggccctata cttcctgttg
     1501 ctcctgtatt tccggttggg cctgttggtc ctatgcttcc tgtcgctcct gtacttccgg
     1561 ttgggcctgt tactcccgtt ggtcctgtac ttcctgtcgc tcctcttggt cccattggac
     1621 ctgtaattct aattggaaga cactcacatt taggataaca attatctata attctatctt
     1681 caaatgttac aagtatatac ggtttgttac aacctctact agatgtaaat tcaagaatct
     1741 gataagggta atttaaagat aaagctaacc cataattagg atatttattc attgcccatg
     1801 cttttatagt tcccgtaata tttaaacaaa tatagttgga tgtatcactt atcccaactg
     1861 gtaaatatcc cctaatattt tccatagaca ctctatcatt ccatgttata gttcgtaaat
     1921 tataatattc acttattctt ttaatttcaa tatctacttt ttctttaaaa atatgattct
     1981 tatttgaatc aatatgaata aataactctg cttttaatat acttttgtct tttaatatat
     2041 ctggtattct cactttaaaa aaagtaatca acttttcttt tacaaaacta tttgaacatt
     2101 tacttcctat taagcaataa tctaaattac taaagttttt atctggatat tttttagata
     2161 taaaagtatc atcatttaaa taaagtataa tatttctcat ttataatccc tccattttgg
     2221 gtctatatca tactatgttt aaaatacatg cttagttaca atataattaa aatttatagt
     2281 attaaatttt tatataataa tgctgatttc tacaaaaaaa gaacatgtca ttagaaattt
     2341 ctaatagcat gttctttttc attattatat attacacttt aaattattcc ttgactggct
     2401 tagctggtgt aaactaaacc agtctttcta aaatattaag cacttttttc ttattatgtt
     2461 ttttaggatt tattgtctat attattgtaa accaaag
//
LOCUS       DAYAOY010000210         2452 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.217, whole genome shotgun sequence.
ACCESSION   DAYAOY010000210 DAYAOY010000000
VERSION     DAYAOY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.217"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..422
                     /locus_tag="ACGI6B_004000"
     CDS             <1..422
                     /locus_tag="ACGI6B_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFG9753401.1"
                     /translation="QQDKTTYIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAI
                     DEAISNGVKNYKYVKTILNNWIEAGVKTNLELTEYQNEFERKKKNKQEKKQSNHKNVN
                     TPNVSKNKFHNFNETFTQYSPDELDDIIKKSQKVKFK"
     gene            482..655
                     /locus_tag="ACGI6B_004001"
     CDS             482..655
                     /locus_tag="ACGI6B_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFG9753402.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            671..1078
                     /locus_tag="ACGI6B_004002"
     CDS             671..1078
                     /locus_tag="ACGI6B_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9753403.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKAKPVYAIGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVLPGLDPQGFQAIDDDDIPF"
     gene            1153..1563
                     /locus_tag="ACGI6B_004003"
     CDS             1153..1563
                     /locus_tag="ACGI6B_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFG9753404.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREVYSGVYLPKNNEN
                     FEVMIYTGLKDCDGKEIYEGDIVLCRSIFFTEFAGEVKFKDGCFIAVNEMSGDCFRLS
                     ELAIVKIDGNIYEDLSKLNDIRRCDEGEIRDDNN"
     gene            1550..1807
                     /locus_tag="ACGI6B_004004"
     CDS             1550..1807
                     /locus_tag="ACGI6B_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753405.1"
                     /translation="MIIIRSQDRLDLMRVNRVEISNKQVCAVFEEESNAREIGRYKSE
                     ERAIEVLNMIQNAVIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            1821..2186
                     /locus_tag="ACGI6B_004005"
     CDS             1821..2186
                     /locus_tag="ACGI6B_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753406.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVHGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            2186..2317
                     /locus_tag="ACGI6B_004006"
     CDS             2186..2317
                     /locus_tag="ACGI6B_004006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753407.1"
                     /translation="MEVSRTEYTIKRAKELYDNGEDIFIAIDKAREEYEEMIKSEFN"
BASE COUNT         1005 a          216 c          520 g          711 t
ORIGIN      
        1 ctcaacaaga taaaaccacc tatataaaaa aatactttga aaaatatata ggtgtgatta
       61 ctcctaataa ctttatagag ttaatgagtt acttagatga tggaatggaa gctgatgtaa
      121 ttataagagc tattgatgaa gcaatatcaa atggagttaa gaattataag tatgtaaaga
      181 caatattaaa caattggata gaagcaggtg taaaaactaa tttagaactt acagagtatc
      241 aaaatgagtt tgagaggaag aaaaagaata aacaggagaa gaagcagtct aatcataaaa
      301 atgtgaatac tcctaatgtg agtaaaaata agtttcataa cttcaatgaa acctttactc
      361 aatattcacc tgacgaacta gatgacataa ttaagaaaag tcaaaaggtt aaatttaaat
      421 aaaattatac ttctaggaag taaatatcaa tatattactt cctagaaagg ggaggtataa
      481 aatggcgaga atatatgcac aaagaagtgg ttctttaaac gaacaagata gattggaatt
      541 attaagatta cttgggaaag ctggatatac agtaaagatt gctagagaga agcaaaatag
      601 caagacaact tatacttact ttgttgagta tacagaagag caggaagaaa aatagaaggg
      661 ggctagttaa atgaatacaa taactttagt tggaagatta gttgcagatg cagaattgaa
      721 gtaccttcca aattcgggta ctccaaaaat aaccttttca atggcagtag atagaaggtt
      781 taaagataaa aatggaaata aaataactga ttttattcaa tgtgaacaat taggaaaaca
      841 tgtagagaat ctagtgcaat atcttgttaa agctaagcct gtctatgcta ttggagagtt
      901 aaatatatat aattacaaag atgaaaatgg ttgctggaaa tctattacta aggttaatgt
      961 aaatgcctta gaactacttt ctagtaaaaa tgataataat gctaaacaag aatatgtact
     1021 accaggatta gacccacaag gttttcaagc aatagatgat gacgatatac ctttttaatt
     1081 aagttaaata gtctaggggg tagttataca atattacttc ctaggagttg aaaaatattg
     1141 gagggatgaa agatggagtt aaaatttaga gaatggaata aaaatggtaa agaaatgtat
     1201 agttatgatg aaatggtgtg ctattctaaa aatttgctta gagaggttta tagtggtgtt
     1261 tatttgccaa aaaacaatga aaattttgag gttatgatat acacaggttt aaaggattgt
     1321 gatggaaaag aaatctatga aggcgatatt gttttatgta gaagtatatt ttttacagag
     1381 tttgcagggg aggtaaaatt taaagatggt tgttttattg cagtgaatga aatgtcagga
     1441 gattgcttta gattatctga acttgcaata gttaagattg atgggaatat atatgaagat
     1501 ttaagtaagt taaatgatat aagaagatgt gatgaggggg agattaggga tgataataat
     1561 tagaagtcaa gatagattag atttaatgag agttaataga gttgaaataa gtaataaaca
     1621 agtatgtgca gtgtttgaag aggaatcaaa tgctagagaa ataggtaggt ataaatcaga
     1681 agaaagagct attgaagtat taaacatgat acagaatgct gttattgcag gaactaagtt
     1741 tgacattata aataaagacg gggttagatg caacaaagaa aaagtgtttg aaatgccagt
     1801 tgaataagga gggagctgaa atgttaaagg ttgaaaaata ttttaatggt tctgttgcag
     1861 ataacatatt tgaagatgat ttgactctta gaaactactt agcactatat tgttgtgttc
     1921 atggagtaag aaaaaatgga gagcttgtat ttccaacttc tgaaaaaatg ctaatagagt
     1981 ttaatgttga taaaaataga aaaaagaaaa agaaaggttc aaaggtaaag ttgattaatg
     2041 ccaaaactgg tgaagaaaaa atatttgatt ctatagatag tgcagcgtgt tttttaagac
     2101 tacagagtca ggcagtttac caaacaatta aaaagaaaac taaaacaaga agtggctgga
     2161 aagctgaata tattaaggag gaataatgga agtttcaagg actgaataca caattaaaag
     2221 agcaaaagag ttgtatgaca atggagagga catatttatt gctatagata aggctagaga
     2281 agaatatgag gagatgatta aaagtgagtt taattaagta cagaggttat gattttgaga
     2341 atgagaagtg gatttattct gaaacaataa agtggagtga tgcagtagat tgtttattta
     2401 tgctaaaaga gaattgcgaa tggcaaaaag tatctaatgt tggagtattc tc
//
LOCUS       DAYAOY010000211         2424 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.218, whole genome shotgun sequence.
ACCESSION   DAYAOY010000211 DAYAOY010000000
VERSION     DAYAOY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.218"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            269..>2424
                     /locus_tag="ACGI6B_004007"
     CDS             269..>2424
                     /locus_tag="ACGI6B_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9753408.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSGGSSSNQTSVNNEAAKITSVPTPAKDATRLTMPSVSSGYTIAIKTSSNE
                     SVIKKDGTIIPPNTATTVKLVFTVTHTSSGKTADTKELSVTVPAKSTDEELQAALDNE
                     VAKITSVPAPAKDATKLTMPSVSSGYTIAIKTSSNKSVIKEDGTIIPPNTEETVTLVF
                     TVTQESSGKTADTGEIDVVVPAKSTDGEIQAEVQAEA"
BASE COUNT         1054 a          327 c          435 g          608 t
ORIGIN      
        1 atacaaaacc ctatttttta taacaattca ttaacataaa aatcaaactt accttaaaat
       61 ataaatttac aaatttttca atcaataaga aaatttcttt attccttaca taatgactgc
      121 aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa cctctttaaa
      181 attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa ttaagtaata
      241 tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg ctgcaatgct
      301 tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac aaactgtggc
      361 aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc aagatggatg
      421 gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg ctctatctgc
      481 aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag ataaattaaa
      541 tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact taataggtgg
      601 aacttctgta ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa tatcttttga
      661 gagaatctct ggagctgaga gatatcaaac atccttagaa ttagcaaaga aattagatgc
      721 tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg cagatgctgt
      781 aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg attctaaaaa
      841 tggaacagca gttgcagaca aatttataaa ggatgctgga ataactcaat cttatgtagt
      901 aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata gtacaagact
      961 aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt ataaaaagac
     1021 agatttaaaa aatgcttatg taactaaaga tggtatgaat aaacaagacc aacttataga
     1081 tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt tagttggaaa
     1141 gaacttatca gcttcacaaa aatctctagt taactctaag agttttgata agataactaa
     1201 agttggtggg aatggtaatg agacagcatt taatgaaatg aagtcattac aagaagtaaa
     1261 aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag ctactgctaa
     1321 tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa tacaaactga
     1381 taaagcagta acagtaaact taaatggtac atatacaaag actgtgacta tcaacatgcc
     1441 taatggtgat gtaaataact atgcaaaagt ggatgatgta gttatagatg atgtaaaaga
     1501 tggaactttt gttaattatg gaaaaataac taacttaaag gttaatgata aaaatggagc
     1561 caagatagaa aataattcta aaggtgagat tggtagttta acagtagcat ctggagcaag
     1621 tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt ctaaagggac
     1681 tactatagat aataaaggta caataagtag tgttaaggga gataattctc caacaataag
     1741 tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag gttcttcaca
     1801 tggaggaggc tcatcttcag gaggaagttc ttctaatcaa acatcagtaa ataatgaagc
     1861 agcaaaaata acaagcgtgc ctacaccagc aaaagatgca actagattaa ctatgccatc
     1921 agtatcaagt ggatatacta tagcaataaa aacatcaagt aatgagagtg ttataaaaaa
     1981 agatggaaca ataatacctc caaatacagc aacaacagta aaattagtat ttacggtaac
     2041 acatacatca agtggtaaaa cagcagatac aaaagaatta tctgtaacag taccagctaa
     2101 atcaacagat gaagaattac aagcagcatt agacaatgaa gtggcaaaaa taacaagcgt
     2161 tcctgcacca gcaaaagatg caactaaatt aactatgcca tcagtatcaa gtggatatac
     2221 tatagcaata aaaacatcaa gcaataagag tgttataaag gaagatggaa caataatacc
     2281 tccaaataca gaggaaacag taacattggt atttacagta acacaagaat caagtggtaa
     2341 aacagcagat acaggagaaa tagatgtagt agtaccagcc aaatcaacag atggggaaat
     2401 acaagcagaa gtacaagctg aggc
//
LOCUS       DAYAOY010000212         2394 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYAOY010000212 DAYAOY010000000
VERSION     DAYAOY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.219"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            51..1763
                     /gene="ptsP"
                     /locus_tag="ACGI6B_004008"
     CDS             51..1763
                     /gene="ptsP"
                     /locus_tag="ACGI6B_004008"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFG9753409.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT          942 a          234 c          454 g          764 t
ORIGIN      
        1 taaatttata aagtttatgt gctagatgat attttttagg aggtagggtt atggcttaca
       61 aaggaatagg tgcttctcct ggtgttgctt tagggaaagc gttagtagtt gaacatagtg
      121 aattagtaat tgaaaaaaaa tctatagata atgttgaagc tgaaattgct aaattagaag
      181 atgcagttgc agtttctaaa gaagaattag taaaagttaa ggaaaaggct tctgaagaat
      241 taggagcaga ggaagcagaa atatttgaag cacatttatt agttttagaa gacccagagt
      301 taattggttc tgcaatagat aaaattaaaa ctgaaagtgt aaatgcagag tatgctttaa
      361 atgaaataaa agagatgttt gttagcatgt ttgagtctat ggacaatgag tacatgaaag
      421 aaagagcggc agatataaaa gatgttacaa atagaatact tagacatata ttaggaatta
      481 aagttgttga tttatctgct ttaagcgaag aagttgtatt aatagcacat gatttaactc
      541 catcagacac agcaactatg aataaaaaaa tggttttagg atttttaact gatataggtg
      601 gaagaacttc tcatactgct atcatgtcta gaactttaga aatagctgca atcgttggat
      661 taaatgatat aacttcaaaa gttaaggatg gagattttgt ggttttcaat ggagacacag
      721 gtgaagtaat cgtgaatcct gatgaagaga ctataaataa atatactgaa ttaaaagcta
      781 agtatgaaga tgagagaaaa gcattacagc tattaaaagg aaaaccatct gtaactttag
      841 acggaaaaca tgtggaatta gctggtaaca taggaacacc taacgatata gaaggtctaa
      901 taaagaatga tgctgaaggt gttggtcttt atagaacaga atttttatac atggatagag
      961 attctttccc aacagaagaa atacaatatg aagcatataa agctgtatta gaaggaatgg
     1021 atggaaagcc tatagttata agaacattgg atataggtgg agataaagaa ttaagttatt
     1081 taagtatgga acctgaaatg aatccattct taggttacag agcaataaga ctttgcctag
     1141 atagaaaaga tatatttaag actcaattga gagctttata tagagctagt gttcatggaa
     1201 gattaagaat catgttccca atgatttcat ctttagaaga gttgttacaa gctaaagaag
     1261 ttgtaaaaga agttttagca gaacttgatt ctgaaggtgt ggcttatgct aaagatgtag
     1321 aaattggtat gatgatagaa gttccatctg ctgctgtgat atctgatgta ttggctaaac
     1381 acgtagattt cttctctata ggaactaatg atttaattca atacacttgt gcagttgata
     1441 gaatgaatca aaagataagc tatctttaca atcaatttaa tccagctgtt ttaagactta
     1501 taaagactgt tatagacaat gcacataaag aaggcaaatg ggcaggtatg tgtggagaat
     1561 ctgctggaga ccaaaaaatg ataccaatac ttttaggtat gggattagac gagttctcta
     1621 tgtctccaat atcaatactt cctgctagaa agttaataac ttcagttaaa gagtcagata
     1681 tgaaaaaatt agctgatgat gtacttaata tgggtactgc tgaagaaata aaaagctata
     1741 tagaaaagac ttttaatata taattaatgt tatatagtta ctaaaattca tttttctaaa
     1801 taacaaaatt atattttacg aattaaaaag gagatgtttc aaaataaaaa tttgaagcat
     1861 ctctttttta taaaagatta ctaagaatta cttaatcttt gtaatagtca ttttgtgaaa
     1921 aagcaaaact gcattgtggc tatagtatat atggatgtta ttatcaataa aagtactatt
     1981 aaaatgaaga ttcattctca aatgatatca aaagtagttg tgatggagta agataatatt
     2041 ttgtgattta atagtaaaaa ttaaaaaaat atggtattga aaaataatta aaagtattta
     2101 ataatagaat tagggaagaa taagtttata agtaaaaata attgattact agttaataaa
     2161 ataaatgtga tatccaaata aatcctaaat ctaatttgag ttttaatata gatagtctaa
     2221 agtttgatta ttaaagattt aattgtaagt tgttacagtt gatgatattg attagagatt
     2281 aaagtttaga gtagaaagtt accttaaatt taaaaatgca agataaccca atctaaaaat
     2341 tataatcact caataggttt aaggatttat ttgtttatta taaataggag ggtg
//
LOCUS       DAYAOY010000213         2384 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAYAOY010000213 DAYAOY010000000
VERSION     DAYAOY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.220"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            272..2284
                     /locus_tag="ACGI6B_004009"
     CDS             272..2284
                     /locus_tag="ACGI6B_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9753410.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1058 a          227 c          342 g          757 t
ORIGIN      
        1 caatagaatt tattaaaata aatttttttg ggtgtatatt ataatttaat cgaatagaaa
       61 tgaaaaatat acatacagtt taagttttaa gcaacaatta gggggatttg gtgacaagat
      121 ttttaattat aagaaagaaa gtgtcttaat gcaactttta taaagtcatt atagacactt
      181 tcttttttat ttgataaatt taaattatca aaattatatt gctttataca actattttgt
      241 tggaaactat ttattatata aggagaaata aatgaaaaaa tcagtagctc tggtaaatga
      301 tagtagaaaa gatttaatag actttttaga aaataatttg aaattagttt ttggagattc
      361 tataaatatt aatagatact tcataaatga aataaatgat aatgatatta taaatgatga
      421 tgtgatatta gtaatgtcag tagaaagatt agacaaaatt ataaataata tactagataa
      481 gaaaaaagtg attgtggtaa gaagaacttt tagggaagat aaaatttata atctactatc
      541 tttaccacag ggtacaaatg ttctaatcgt aaatgattct gatgaaacta ctctagaaac
      601 tataagttta ttttataaaa ttggagtgac taatattaga ccaattccat atatgaatga
      661 taataactat aaaaatataa aaatagctat aactccagga gttccagaaa aagtacctag
      721 ctttatctca gatatatttg atttaggtca cagatatata gatatttcta catttataga
      781 aataataaat ttattacaaa ttgattcaaa ggaaatacaa tctaatctag ttaaatactc
      841 agaagaaata ataagtttag atactggtat aaaagataaa tataaggaat tatttttaaa
      901 aatagaagaa ttagatacaa tattgaattt atcaaaagat ggtatattat ttacttcaaa
      961 agatggtgaa ataaatacat ataatagtaa agtaaaagat attttagaca taaatgagga
     1021 tatatatgga aaatacattg aggatatatt tgtagatagt ttaaaagttt tattgagtga
     1081 gaaagagata ctagataaag ttgtcgtatt caataagaaa tatataaatg taaataaaaa
     1141 aaacatatac aatagagatg aaaaaatggg cacatattat agtttacaag aaataacata
     1201 cataaaaaaa ttagaacaaa atctcactaa aaaattaaga gaaaaaggac aaatagcaaa
     1261 atatacattt aaggatataa aaacaaatag tcctaaaatg tttgaatgta tagatttagc
     1321 taagaaggtt tctaaatctg acttaagcat acttatcaga ggtgaaagtg gaactggtaa
     1381 agaactcata gctcagtcta tacataacaa ctcaaataga aaaaatcaac cctttatagc
     1441 agtaaattgt gcagccgttc ctgaaaattt attagaaagc cagctattcg gatatgacaa
     1501 gggaactttt actggaggtc taaaagatgg aaaacaagga ttatttgaac ttgctaataa
     1561 tggaacaata ttcttagatg aaataggcga tatgccttta gaattacaaa caaaactact
     1621 tagagttctt caagaaaagc agataatgcc tgttgggtct cacaatgtaa ttaatataga
     1681 tgttagaatc atatctgcta caaataagaa tttagaacaa atgattgata atagccaatt
     1741 tagagaggat ttatattatc gattaaatac aattccaata aatatacctc ctctaagaga
     1801 aagaaaagaa gacatattaa ttattatgga agatttaatt aataagaaat tagtaatcac
     1861 accagaagca aaaaaactta tacaaaacta tatgtggaaa ggtaatataa gagaactcca
     1921 aaatgttaca tcctatttaa acattatgtg tgaagatatt gtattagaga aagatttacc
     1981 tccaaactta aggtcttcag ataacaaaaa tacatcatta aaactaaaat atagtaagaa
     2041 tgatatattg aatatattag agatactaat tttaaataag gaatcagatg ttggtattgg
     2101 tagaggatta attttaaaag cactactcga taagaacctt caaattacag aaggaaaaat
     2161 taaaaaaata tttgaatatc taaaaaaaga agaacttata atttgtagtt caggaagata
     2221 tggaagtaaa ataactcaaa aaggagaaga tttttacaat aaattaaaat ataaaggtct
     2281 ataaaattat aatttatcaa acaaagaaat ctattcagga ttaaagtact ccttcaatag
     2341 gtttttttat ttttcaagta ccctgaaatt ataatttatt ttgt
//
LOCUS       DAYAOY010000214         2289 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYAOY010000214 DAYAOY010000000
VERSION     DAYAOY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.221"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(178..1470)
                     /gene="brnQ"
                     /locus_tag="ACGI6B_004010"
     CDS             complement(178..1470)
                     /gene="brnQ"
                     /locus_tag="ACGI6B_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9753411.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          766 a          381 c          247 g          895 t
ORIGIN      
        1 cttttttatt ttattatctt tataaatcat actacttcaa aaataaattt ttaaatttat
       61 caaacaggat taataatgtt cttttatcta caataattta ataattcttt taaatataaa
      121 aaggaattgc ctatataatt taaaggcaat tccataatta tcaattagtt tttattatta
      181 tgcagtttta ctgcttttag atggaataaa gtttcctatt attcctgcta ttacaactgg
      241 aactatccag ttaaatccta atgaagcaaa tggtaagcta ttaactagag gaatttttac
      301 accaaaactt gttgcaactg ttaatacact tactaataat gccatgtatg cagcacattt
      361 gaacacgttg tcatttttaa ttttattact aaataatgct agtataacta gagttatagt
      421 tgctggatat accatactta gtataggaga agagaattgt attattgtac ttactccaaa
      481 gtttgatatg attgcactaa atacacaaac tactataact atatgctcat actttaattt
      541 tccatctgta agtgttgaga aatattgacc agctgctgaa gaaagaccta tagctgttgt
      601 taaacatgct aatgcaacta taatagccaa taaaatttta cctgtagttc caagtaaagc
      661 tgctgttatt gcaactatta aagctgtttg ttgaacatct tgtccatatt gttgagacac
      721 agttgctcca agataacata atccaccata aactaatgcc attcctatta ctgcaattac
      781 acctgcactt attgtggctt tcatcttttc tttctcacct gtataacctt tagctattac
      841 tgacatgatt ataattgatg caaatacact agctgccaat gcatccattg tttgataacc
      901 ttgagttata ccttctgcaa atacattttg tattaaagaa ctgtctctta tttctcctaa
      961 tggagatacg atacctttaa ctattaatac tgctaaagct ataagtaaag ctggagttaa
     1021 tattgaccca actatatcta caacttttga tggtcttatt gttaaaacca atactagagc
     1081 aaagaatact atagagaata ttactgggct tattccagaa cctattgtag gtaatatacc
     1141 catttcataa gttgttgctg ctgttctagg tattgctaaa agtggcccta tacagattac
     1201 cattgcacat cctaatgcaa tacttaatcc tttgcctgcc cttgagaaca tcttcgatgt
     1261 gtctccattg aacttagttg ctgctataac tgctaataat gccaaaccac catctgcaaa
     1321 aaggaatcct atgaaagcta ctagccaact acttccagat aaaactccta gatatggagg
     1381 gaagattaag ttccctgcac caaaaaacat tgcaaatagt gcaaatccaa ttacaattac
     1441 atctttaaaa ttgatttgtt tctcacccat ttaaagcacc cccatttgaa ttttactacc
     1501 ttttactaaa aaataagtcc aatctcttaa ttaaatgaaa accttttgac gagattttat
     1561 ttaattatac atttttgact taaatttgtc aattatgttt cataatttat aaaatcttca
     1621 caatatttaa tacaactttc ttaattaaac aaaatcaacc tgtatacttt aaacatagtt
     1681 ggttttttgt tttttctcta atttttcttg ataaaattaa ctattccgaa taattaaaat
     1741 attaagactt tttagattat tcgatttatt attaagttgt attgctttat atactgtata
     1801 tatcactctc cttttttaca ttcatataat ctttttaatt caatttaaat tttaaatatt
     1861 ttgtgtttat tatataatct gaaaaaaaag cagtcaatag catttttaat ttttttaatt
     1921 ttcaaaatta tgattcttaa aaaatcgttt tccttttatc ataacaccta tttacgttaa
     1981 ttcttgtagt attttgtatt tattttgcta aattgacagt taactttatt caatttttat
     2041 tctacttgcg tagatttgcc tcttgttttt tactcttaaa accatttttt ctgaaaattc
     2101 cttattatga tttatcaact tatttttctt cataacaatt actctctaac ttccaactta
     2161 cttaaacttt gcagttgcta gtattcacgt ttacatactt atatgtcata attaactaaa
     2221 aattcttaat atatctctca atgaaaacaa tttactcatt tatttaatac atttatataa
     2281 tcatatgtc
//
LOCUS       DAYAOY010000215         1925 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.223, whole genome shotgun sequence.
ACCESSION   DAYAOY010000215 DAYAOY010000000
VERSION     DAYAOY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.223"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="ACGI6B_004011"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="ACGI6B_004011"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="ACGI6B_004012"
     tRNA            242..316
                     /locus_tag="ACGI6B_004012"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="ACGI6B_004013"
     tRNA            321..395
                     /locus_tag="ACGI6B_004013"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="ACGI6B_004014"
     tRNA            401..476
                     /locus_tag="ACGI6B_004014"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="ACGI6B_004015"
     tRNA            482..558
                     /locus_tag="ACGI6B_004015"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="ACGI6B_004016"
     tRNA            569..643
                     /locus_tag="ACGI6B_004016"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="ACGI6B_004017"
     tRNA            658..742
                     /locus_tag="ACGI6B_004017"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="ACGI6B_004018"
     tRNA            752..825
                     /locus_tag="ACGI6B_004018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="ACGI6B_004019"
     tRNA            836..912
                     /locus_tag="ACGI6B_004019"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="ACGI6B_004020"
     tRNA            922..997
                     /locus_tag="ACGI6B_004020"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="ACGI6B_004021"
     tRNA            1009..1084
                     /locus_tag="ACGI6B_004021"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="ACGI6B_004022"
     tRNA            1087..1175
                     /locus_tag="ACGI6B_004022"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="ACGI6B_004023"
     tRNA            1193..1283
                     /locus_tag="ACGI6B_004023"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="ACGI6B_004024"
     tRNA            1292..1368
                     /locus_tag="ACGI6B_004024"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="ACGI6B_004025"
     tRNA            1454..1529
                     /locus_tag="ACGI6B_004025"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="ACGI6B_004026"
     tRNA            1590..1666
                     /locus_tag="ACGI6B_004026"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="ACGI6B_004027"
     tRNA            1673..1749
                     /locus_tag="ACGI6B_004027"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="ACGI6B_004028"
     tRNA            1753..1828
                     /locus_tag="ACGI6B_004028"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="ACGI6B_004029"
     tRNA            1836..1912
                     /locus_tag="ACGI6B_004029"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAYAOY010000216         1818 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAYAOY010000216 DAYAOY010000000
VERSION     DAYAOY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.224"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            169..1767
                     /locus_tag="ACGI6B_004030"
     CDS             169..1767
                     /locus_tag="ACGI6B_004030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HFG9753412.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          698 a          221 c          278 g          621 t
ORIGIN      
        1 tatataataa taatttttat attaaatttg tagtatttat gtaagaaata aattttatat
       61 aatatttttt cactaaattt ttttcttttt acaacaaata ctcactacct tttgatataa
      121 tatttttatc aattattatt atattcaagt tttgaaagga gtattaaaat gagtttatta
      181 gttttcggac ataaaaatcc agatacagat tcaatatgtt ctgcaatttc attagcacat
      241 ttaaagaaca aactaggaat aaaagcaaca gcttatgctt taggagatat cagaaaagaa
      301 gctaaatatg ccttggatta ctttaaggta gatgcacctg aaattttaga taatgttaga
      361 atacaagtta gagatttaaa ttatgataaa gtagtacctt taacaccaac ttcatctata
      421 ttagaggctt ataaccttat ggatgaaaaa aatgttaaaa cactacctgt aacttatgat
      481 gatggaactt ttgctggtat cattactatg aaagaaatag ctaaaaatct acttcatcaa
      541 cactttacaa ctatacacac atcactatca aacatttgta aaaaccttaa tggtactatt
      601 cttgtggact gtggtgaaga tataaaaggt agagtagtca ctttatcatt tggaatggag
      661 actctaatag agactttaaa agaaggagac atagctattg ttggagatag atatgatagt
      721 attgaatatg ctattgatac aaaagttaaa cttttaatac ttactaatca tacagagatt
      781 tcaaaagatt tgctagctct tgctaagata aatggagtgt ctgttgtttc tgttgaaagt
      841 gacaattata aaacttctaa tgtaataaat caatgtagtt tcttagactc tatagaagct
      901 acagaaaatt taattacatt caaagaagat gattatatgg atgaaataaa agagattatg
      961 ctagaaacaa acttcagatc ttatccaata ttagatgatg ataataagtt tttaggttta
     1021 gtttcaaaag gtcatctatt aaacccatct aaaaagaatg ttgttttagt tgaccataac
     1081 gaatatgctc aaagtgctga tggaattgaa caagcaaaca tagttgaaat aattgaccat
     1141 cataaacttg gtggtatttc tactgatgtt cctatgtcat ttagagttat gcctgttgga
     1201 tgcaatagta ctataattta tcaaatgtac aaagaaaata atgttgagat accttatgaa
     1261 atagccgggc ttcttttatc tgctatatta tcagatacat tattatttaa gtctccaaca
     1321 acaactgata tagataagaa agcttgtgaa gaattaagta aaatagcaaa agtagacatg
     1381 gaaaaatacg ctatggatat gtttaaatgc ggtacttctt tagatgaata tactatagaa
     1441 gaaatagtaa atatggactt taaagaattt aatatgagtg gacatagagt tggtattgga
     1501 caagtattta ccttagacat agactctata tttgctaaaa aagatgagtt tttatcttat
     1561 ataaattcga ctgattatga caaactaatt cttgcggtta cagatataat caaagaaggt
     1621 tcttatttaa tctataaagc agaagacagc ttaattgcta acgcatttgg tgttgaaggt
     1681 gttcaaggta catttgcacc aggtgttgta tcaagaaaaa aacaattagt ccctaattta
     1741 actactgcaa tcaaaaattt taaataatat aaaattttta aataacgaaa actgtctcaa
     1801 atctatattt gagacagt
//
LOCUS       DAYAOY010000217         1684 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYAOY010000217 DAYAOY010000000
VERSION     DAYAOY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.226"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            387..1214
                     /locus_tag="ACGI6B_004031"
     CDS             387..1214
                     /locus_tag="ACGI6B_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFG9753413.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          600 a          210 c          224 g          650 t
ORIGIN      
        1 cttctatctt ctatcttcta tcttctatct tctatcttct atcttctatc ttctatcttc
       61 tatcttctat cttctatctt ctatcttcta tcttctatct tctatcttct atcttctatc
      121 ttctatcttc tatcttctat cttctaaata attttaacat gcccataaaa tataataccc
      181 acaaatgata aataatactc aaaatgtcta ttgaaataac tatacatcct taaataactt
      241 aacttaaatc taaacatttc aaaaacttct taaaaatatg taaaaatttg atgataaagt
      301 agcgattttt tcaaaaaaaa tgtaatatag agttatatct tataatattt tagagataaa
      361 ttttatcata gtggggagaa atttgtatgg atttaagcaa aaaagtaaaa attttagaaa
      421 atgcatcaga tggcaaaagc gaatttgaga tattagaatt tgaaaaactt gagggagctt
      481 ctagcccatt aatggcaatg ggattatatt ttgctagaga agctggtttg aaagctagac
      541 aaattaagat aaaattaaat aattcatcaa taaaaacaga agctggtgca ttatactatt
      601 ataaaggaaa tatatcttct gaaactaaga ttggtggtgt tggaggtctt tttaaaaaag
      661 gaatatcagg agcacttact aatgaatcaa tgatgaaacc tgtatacaaa ggtactggag
      721 aagtttattt ggaaccttct tttagacatt atttatttat gagtcttgat aatgattcta
      781 taattgttga taaaggatta ttctattgtt gctctgatac aatagaagta aaagcattta
      841 cacaaaagaa cttctcatct gctttacttg gtggtgaagg cttcttccaa atagaattaa
      901 gtggtactgg tgtaatcgtt ctggaatgtg ttgtacctca aagtgaaatt gtagagtatg
      961 aattgaaaaa tggtgaagaa ttaaaggtag atggtaactt tgcaatagca agaacatctg
     1021 gtgttaattt ttctgttaca aaatcagata aatctttaat tggttctgct ttaaatggtg
     1081 aaggtttctt aaatacattt actggtaatg gtacagtttg gctagctcca actcaagtaa
     1141 tgtatgaaaa actaagtttt ggagcaatgc ctactaatga taatatgaat aataaagttt
     1201 ctaagcaaaa atagttgttt tataaatatt tattaaatca ttattgattt tataaagtca
     1261 ctataaattt tattagatta ttattataaa atcagagtgt ggaaatgttt atgtgtattc
     1321 tttaaataaa aatttccaca ttctgtaagg ttattatact tgatatataa aattcaattt
     1381 aggaacatag tctaacattt ataaacatat taaggtaatg atttctcttg gtaattattc
     1441 tcatatctta ttctttgatt atatttatct tattcctagc ccttatgtca tattatattt
     1501 cttatcttat tacttataat tagcattaac ttatctaaaa tcttgtattt ttttaatagt
     1561 aaagtttaca tctatattaa aaagtttaca tctatattaa tttcataaaa caatcaaggt
     1621 agtcaagaga ttcaacttta cttgatttta aaaatttaag tgaatttgtt cttatacaca
     1681 tctt
//
LOCUS       DAYAOY010000218         1608 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYAOY010000218 DAYAOY010000000
VERSION     DAYAOY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.227"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            393..1598
                     /locus_tag="ACGI6B_004032"
     CDS             393..1598
                     /locus_tag="ACGI6B_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9753414.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          605 a          204 c          214 g          585 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaat
//
LOCUS       DAYAOY010000219         1601 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYAOY010000219 DAYAOY010000000
VERSION     DAYAOY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.228"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            247..1383
                     /locus_tag="ACGI6B_004033"
     CDS             247..1383
                     /locus_tag="ACGI6B_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9753415.1"
                     /translation="MIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLIN
                     EIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMI
                     YNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIH
                     TGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYLY
                     LIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEMR
                     NMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLKA
                     GIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1409..1597
                     /locus_tag="ACGI6B_004034"
     CDS             1409..1597
                     /locus_tag="ACGI6B_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753416.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          691 a          134 c          228 g          548 t
ORIGIN      
        1 ataatcgaga tattgttaag acattgtcga gatattataa tatataacta atgtattata
       61 aataatagtt gtaaacttcc tgtaacatat attttaaatg cttattctta aatttttgca
      121 ttatttatta aaatttatta tgctttaacg ctaaattgag tttgataatt ataaaacaaa
      181 taatcaaaaa tagtgtaaaa attaatactt tttgttaata ttaatagata aagataatct
      241 aaggtggtga ttaaactgac aatagaagaa tacaaaaaca aatactttac tggaaataaa
      301 atcgtacttt ctataaatag ttttgataat aaagaactat actatcatga aactgaaaat
      361 atattaaatt taataaatga aatagataaa ttaacatcaa gtaagatatt taaaacaata
      421 gaagataaaa atgatttggc actagagtct tattattcta gtgtaataga aggtgctttt
      481 tcaactagaa agattgcaaa atctataatt agaggaaaga tgaaaccaag taataaaagt
      541 gaatatatga tttacaataa tcatagagcc ttagaatatg gattagataa cttagatgaa
      601 ttgtatagtc acaaatttat atatgatttg catcatatat tgggcgaaaa ctgcttggat
      661 tcagaagaat atgagtatag aactgaaaaa gtatatgtat gtgattcaaa aggtgagatt
      721 atacatacag ggcttgaacc tttgaagata tatgacttta ttagtaaatt aatagatttt
      781 atggaaaata gcaaagtaag taatttaatt aaaagtgcta taattcattt ttactttgta
      841 tatgttcatc cttttagtga tggtaatgga agaacatcaa gagccttatc atatctatat
      901 ttgattgata aaggatatga tactttcaag gatttttcaa tatcatatat gatatctaaa
      961 aacagaacta agtattataa ggctatactt gatgtagaaa acaaaggaaa taatttaact
     1021 gtatttatag aatttatgct caagtctata atacaatcta ttaatgagat gcgtaatatg
     1081 catgatagaa aatctttaga aagtatatta aaagaagaat tgtttgaaaa tgacataact
     1141 ctatcagcaa cagaagaaca tatattaaaa tatatttgca ataaagataa ctattctatg
     1201 acattagaaa attatataaa aaagaataaa agtagatact taaaggctgg aataaaggaa
     1261 attgaattag ttgaccagtt gatggaagtg tttaataatc ttgaagagat agagattttg
     1321 tctaaagaaa aaaatatata taaagttaat gataagtact taaaaatgct tgatatagac
     1381 taatttagat aatctggaga gggaatatat gaataataag aaaaaggcat ttttgatttc
     1441 tggggctata aacgtaattt cattaattat tgttttttca ttgtttcatt ataatattat
     1501 agaaaatagt acacaaaata tgataataac tgtaatctta atttttattt ttgaaatgat
     1561 aaaaataaaa atcatagaga aatactataa actataaaca a
//
LOCUS       DAYAOY010000220         1513 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYAOY010000220 DAYAOY010000000
VERSION     DAYAOY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.229"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..571
                     /locus_tag="ACGI6B_004035"
     CDS             <1..571
                     /locus_tag="ACGI6B_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753417.1"
                     /translation="FALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQFL
                     IYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTSFF
                     SKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTIDSI
                     GNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            574..>1513
                     /locus_tag="ACGI6B_004036"
     CDS             574..>1513
                     /locus_tag="ACGI6B_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753418.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPF"
BASE COUNT          538 a          182 c          194 g          599 t
ORIGIN      
        1 ctttgcttta gtttcactag gatttcttgt aataattttt ataatgaaca atattttgtc
       61 aaattatata agtgaatcaa agggactttc aaaaagactt tattctatgc ctataactag
      121 agtacaattc ttaatttatg actttgtagg tttatggatt tattcattta tattcttatt
      181 attatatgtt ttattcttta gaatacttgg aataacattt aaaggtaatt ttgccatatt
      241 gctcctactt tgtgctttat catcttattt tatgacttca atatctacct ttgtaaccag
      301 cttttttagt aaaaaatacg gtacagcaat agtttatgca ttactctttt tacagacaat
      361 ttttggaggt atcttcagta tgataagtga tacatttact aagcttacaa gtttatctcc
      421 tacctattta ataggagaat tgtttagtaa ttatgaaaca tttaaaacca tagattcaat
      481 aggaaattta attttcacat gtttaattat ctctacaata ttaattgcac ttgctattgt
      541 aaaggaaaaa tataaatgga gggaggttta ataatgatta gattaactat tgcaaatata
      601 aaaagatatc taaaaaatca tactctttta attaatatgg ttatgcttcc tattatacta
      661 attttttcac ttaatttttt tatcaacaac tcgggaaatc aatcaatgta cttttctcca
      721 gtagcaattg tatctgactc ttcaggaaag tatgaacata agttaataaa tagttctaaa
      781 ttaaaagaaa attcatttag attaaatgaa caagataagg ctatgaatct attaaaaaat
      841 aataaggttt ctgctgtatt cgttttagat aaaaattttt caaatagtat agataaatta
      901 caacgacctg tagttaaatg ctttaaaatt gaaaatggtg gtggtagtct ttgggcagaa
      961 tcacaaatag aatcttttat tactaaatcg ttaaaattga aagtggataa aaatatagat
     1021 gataaattga caaagacaaa tataattgat aacaaaccag ataagaataa aggttcattt
     1081 ttagtagtct tcttgatatg ctattttatg tatattaatg cggcacatct tgcttctgat
     1141 ttatttacat taaaaaaatc aaatgtgcta aaaagactta tctctacaga taataaagat
     1201 ataaaaataa ttttcagtat atttttagga ctatttttta ttcaatcaat tgtatacact
     1261 tttgctctat tatgtttttc aattatagaa gactttaatt tgagtctgaa tgtattgatg
     1321 attgtactag caaatagttt tgtttccact ggatttgtat tttgggtagc tagagtattt
     1381 aaaaatgaaa gttctataag tcttatatct acattttatt ctttaatagg tctagctata
     1441 tctatctcta gtctaatacc atcaatggat aaattatcct ttatgactaa tctttcaaag
     1501 ttcacaccat ttt
//
LOCUS       DAYAOY010000221         1483 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYAOY010000221 DAYAOY010000000
VERSION     DAYAOY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.230"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..276)
                     /locus_tag="ACGI6B_004037"
     CDS             complement(<1..276)
                     /locus_tag="ACGI6B_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HFG9753419.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIV"
     gene            complement(306..1415)
                     /locus_tag="ACGI6B_004038"
     CDS             complement(306..1415)
                     /locus_tag="ACGI6B_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753420.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT          467 a          220 c          184 g          612 t
ORIGIN      
        1 tactataaaa aaaccattgt gttgtattat atttatatct tcaaataaat tataaaaaat
       61 attttttaga tattcaaagt ctatatgaga ttggtaaatt aaataaaagt tgccttttaa
      121 attatcgaga ttatttttat ctagaaaatc aataacataa atagcattat tgaaagtaac
      181 taaatggcta ctagcatcta acttttggtt ttcatatgga tagatattta tttttttatt
      241 aagctgtctt tctaaaaaat cttttaactc ttccataaag tctccttaaa aataagatta
      301 tattcttatc ttatagctaa agtagtttct ttgtcaaata tgtgtactct actattgtca
      361 actgaaagtg ttaagtcatc accaacatta attctgtttt tactgtcaaa tctaattgtt
      421 atcgtatttt cgcctagttt taagtgagca taagtttctg ctcccataag ttcttttatt
      481 tcaattcttg atttaaaaca agtatctgga ttagatttaa taaaagtatc ttctacatga
      541 atatcttctg gtctgatacc tataacaact tcattattaa tataattgtc ttttattaaa
      601 tcatagtgtt tttctttatt caatttaagc ttaatatgtt cattttgtgc ataaatttca
      661 tcattttctt ctattaatat tacatcaata aaattcatct gtggtgctcc aataaatcct
      721 gcaacaaata tattagctgg ataatcgtat acatcttgtg gagttgcaat ctgctgaact
      781 actccatcct tcattactac gattctatca gccatagtca tagcttcaac ttggtcgtgt
      841 gttacataga taaatgtagt tccaagctgt tggtgtagct tagttatctc tgttctcata
      901 gcagttctaa gttttgcatc taagttggat agaggttcat ccattaagaa taccttagga
      961 tttctaacta ttgcacgtcc taaggcaacc ctttgtcttt gtcctccaga taatgtttta
     1021 ggttttttat ttaaaagagg tagtaagtct aatattttag cagcttcttt aacttttttg
     1081 tctatttcat ctttaggcaa atttctaagt ttaagtgcaa atgccatgtt ttcgtaaaca
     1141 gataagtggg gatataaagc atagttttga aaaaccatag ctatatctct atcttttggt
     1201 tctatattat tgacaagttt atctccaatg tatagttctc cttcagagat ttcttcaaga
     1261 ccagcaatca ttctaagtgt agtcgattta ccacaaccag atggtcctac taaaacgata
     1321 aattctttat ctttaatgtc tatatttata ttttttactg cgttaaatcc atttgagtaa
     1381 agttttgata tattttttaa aattacttct gacataatat cctccttgat atttaaattt
     1441 taattatata gtatcaaatt tttttattat attaaatgtg taa
//
LOCUS       DAYAOY010000222         1461 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYAOY010000222 DAYAOY010000000
VERSION     DAYAOY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.231"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1338
                     /locus_tag="ACGI6B_004039"
     CDS             <1..1338
                     /locus_tag="ACGI6B_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9753421.1"
                     /translation="NKAFEENYGLKKEDILGKNVSFLEDSGYSTKSPIPIVLKTKTKF
                     SLEQDTQTGKKLIITATPIFDENGHLEFTVENCRDITELNNIKNKLEDTKKQVKKYKS
                     EVETLYRTALRIEDTVIMDGIVMRPIINTVNHVSKTDVSVLLLGESGTGKSSLARYIH
                     HNSNRSNGPFITINCATISPQLLESELFGYTSGAFTGASTKGKVGLVELANGGTLFLD
                     EIGDIPQNLQAKFLQLIQDRTFTPVGSLKNKKVDIRIISATNVDLVSKVKEKKFREDL
                     YYRLNVIEIKLPPLRERRDNLVEIIKYYFNRYSSDFNLNKTISKEAMDAIANYRFPGN
                     IRELQNIIQKILLTCTDNHITIDDLPNILTKNIHITNNGNKTHISQINKVIISDSKST
                     NYKNKNFDTLIKEYEKNIILDAYEKFGSSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT          617 a          188 c          206 g          450 t
ORIGIN      
        1 aataaagcat ttgaagaaaa ttatggtcta aaaaaagaag atattttggg aaaaaatgtt
       61 tcttttttag aagactctgg atattcaaca aaaagcccca taccaattgt attaaaaaca
      121 aagactaaat tttcactgga acaagatact caaacaggga aaaagcttat cataactgct
      181 acccctatat ttgatgaaaa tggacactta gaatttactg ttgaaaattg tagagatata
      241 actgaactta ataatataaa aaataaactt gaagacacaa aaaagcaagt aaaaaaatat
      301 aaatctgaag tagaaacact atacagaact gcacttagaa ttgaagatac tgtaattatg
      361 gacggaatag ttatgagacc aatcataaac acagtaaatc atgtttctaa aacggatgta
      421 agtgtacttt tattgggtga atctggaaca ggaaaaagct cacttgctag atatatacac
      481 cacaacagca atagatctaa tggcccattt ataacaataa attgtgcaac tatatctcca
      541 caacttcttg aatctgaact ttttggatat acttcaggtg cttttacagg tgcaagtact
      601 aaaggtaaag ttgggctcgt agaattagct aatggaggaa ctcttttttt agatgaaata
      661 ggagatatac cacaaaatct acaggcaaaa tttttgcaat taatacagga tagaactttt
      721 actccagttg ggagcttaaa aaataaaaag gtcgatataa gaattatatc tgctacaaat
      781 gttgatttag tttcaaaagt aaaagaaaaa aagtttagag aagatttata ctatagatta
      841 aatgttatag aaataaaatt gcctcctctt agagaaagaa gggataatct agtggaaatt
      901 ataaaatatt attttaatag gtactcaagt gacttcaacc taaataaaac tatttctaag
      961 gaagctatgg atgctattgc aaattataga ttcccaggca atataagaga attacaaaat
     1021 ataattcaaa aaatcttgct tacttgtaca gataatcata taacaataga tgacttacca
     1081 aacattctta caaaaaatat acatattacc aataatggaa ataaaacaca tatatcccaa
     1141 attaataagg ttattatatc tgactcaaaa tctactaatt ataaaaataa aaattttgac
     1201 actttaatta aagagtatga aaaaaatata attttggatg cttatgaaaa atttggtagt
     1261 tcttataaag tagccaaaca ccttgaaatc tcacagtcta aagccaatag gttaattaga
     1321 aagtatacta atacttaatt ttaaatttga taaataaaaa cttaacacta tttacatatt
     1381 gtaaatttgg ccatttactg ttattatgaa tatggttgaa attatttaaa taaatactgt
     1441 tgacttagtt tcatatattt a
//
LOCUS       DAYAOY010000223         1337 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYAOY010000223 DAYAOY010000000
VERSION     DAYAOY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.233"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            27..101
                     /locus_tag="ACGI6B_004040"
     tRNA            27..101
                     /locus_tag="ACGI6B_004040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACGI6B_004041"
     tRNA            116..200
                     /locus_tag="ACGI6B_004041"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACGI6B_004042"
     tRNA            210..293
                     /locus_tag="ACGI6B_004042"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="ACGI6B_004043"
     tRNA            323..399
                     /locus_tag="ACGI6B_004043"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="ACGI6B_004044"
     tRNA            407..482
                     /locus_tag="ACGI6B_004044"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="ACGI6B_004045"
     tRNA            571..659
                     /locus_tag="ACGI6B_004045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="ACGI6B_004046"
     tRNA            663..738
                     /locus_tag="ACGI6B_004046"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="ACGI6B_004047"
     tRNA            745..818
                     /locus_tag="ACGI6B_004047"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="ACGI6B_004048"
     tRNA            833..909
                     /locus_tag="ACGI6B_004048"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="ACGI6B_004049"
     tRNA            933..1009
                     /locus_tag="ACGI6B_004049"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="ACGI6B_004050"
     tRNA            1017..1093
                     /locus_tag="ACGI6B_004050"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="ACGI6B_004051"
     tRNA            1102..1177
                     /locus_tag="ACGI6B_004051"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="ACGI6B_004052"
     tRNA            1185..1258
                     /locus_tag="ACGI6B_004052"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="ACGI6B_004053"
     tRNA            1265..1336
                     /locus_tag="ACGI6B_004053"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAYAOY010000224         1309 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYAOY010000224 DAYAOY010000000
VERSION     DAYAOY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.235"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(73..1113)
                     /locus_tag="ACGI6B_004054"
     CDS             complement(73..1113)
                     /locus_tag="ACGI6B_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFG9753422.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          460 a          197 c          147 g          505 t
ORIGIN      
        1 aatttttgta taaaaataaa aattaaagtt aattatttgt taagaattga ctttggtaaa
       61 atatataatt aattattctt taagcatata atttataagt aattttagtg tattttcaat
      121 agcttctata tgtgttcttt cataatggtg actattgttt gttccaggac caacacatgc
      181 tatttgaaca tcttctcccc aacgaatagc ttctgaagca tctgaaccat agaaagtaaa
      241 tacatcgaca gcgtagtcta aattattgtc ttctgcaaca tcgacaagct tgtttcttaa
      301 attaaaatca tataatgata aattatcttt tgcaactata gttacaccat gttcactaga
      361 tgtttgactt ggtccagtag gtgctatatc tattgctaca aattcttttg ttttttcagg
      421 taaacattta gaaacaccat gtccaatttc ttcataatta cttatataaa agttagtagt
      481 atgcttaggt gttaaattgt tttctttaaa atatctacat atttcaagaa ccattgctac
      541 agctgattta ttatctatat atctagattt tataaaacca gattctgtag cctcaaatct
      601 atggtctagg catacaaaat ccccaacact tattcctaaa tctaatacat cttgctttgt
      661 aaatactttt tcatcaagtc taataatcat tgtttcttca tttctttcag tggcagcttt
      721 tgtttgacca tatatatgag tagaagcata cttaaataca acacttcctc tgattttttt
      781 accttttcta gttataacag tacagttttc tccctctata gccgcccaac atccaccacc
      841 aactttatgg tatttaagag taccatcaga tgatatttct tttactatag ctccaagtgt
      901 atccatatgt gcagatattg ttatttgatt tgaatcatct tcaccaggaa gagtagctat
      961 aagtgcacct ttttttgtaa gaatagtgtc aagacttaaa ttttcaaaat cttttttgat
     1021 ttctaaaact gcattttcag tatatccagc tgggcttgga atgtccaaat attttttcat
     1081 taaatttata gtattagtta aatttaaatt catattgttt cctcctaaat aaatatattt
     1141 atacaaatta taacataaat acaaatgttc tgaaaactaa aattaaaaga atagaatcta
     1201 aacatattta aaaaaaaagt atataaaata aatttgattt atattatata gatttaaagt
     1261 aaaaatagtt ttatttaata attaataaaa ataaatacaa aacaaaata
//
LOCUS       DAYAOY010000225         1289 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYAOY010000225 DAYAOY010000000
VERSION     DAYAOY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.236"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..505
                     /locus_tag="ACGI6B_004055"
     CDS             <1..505
                     /locus_tag="ACGI6B_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753423.1"
                     /translation="DNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFAL
                     YERSAQDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMM
                     FENSLDIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVI
                     GEDELNE"
     gene            498..1130
                     /locus_tag="ACGI6B_004056"
     CDS             498..1130
                     /locus_tag="ACGI6B_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFG9753424.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT          526 a          125 c          223 g          415 t
ORIGIN      
        1 tgataatcaa gataatataa ataaagagat tttatttgat ataaaagaat cttcaaaaaa
       61 tatatacaat gccactgaaa gccaattcaa aattagagat tatataaaaa atataaatgc
      121 aggatttgct ctatatgaaa gaagtgctca agatttatta actaaattaa tgacccatga
      181 aaataaaata tctcaaaatc aaaaaatcct tttagatgaa aagtttacct tagatgactc
      241 aataaaaaat ctctcaagta ttattgaaaa cttttctaat gacctacaag ttaaactgga
      301 tatgatgttt gaaaattctt tagatattca agataagtta gatgttatgt ttaataactc
      361 ttttatgaat gatgaagttc ctcttgatag tgaagaatta tttaaggatg atgttaataa
      421 tgtatttaat ccttttagtg aagaaattta tgagatagag gataaagaaa taaaggtcat
      481 aggtgaggat gagttaaatg aataataaat atagaagaac tgttaacaga gaatttgaaa
      541 agagtagctt ttggccaaca tttacagatt tattatctac tgtattgatg gttgtgattc
      601 ttatcttatt tagctcagaa agtatttcag gttctgttga acaagattta gctaagaatg
      661 tcaatgcttc tgttgaagaa acttttaaaa aaagtggaat acctgtgaag gtagataaat
      721 ctaatgggca agtaactttt ggtgaaaaaa ccatgtttga tgtagatagt gatgttttaa
      781 aaccagaggc taaagaaatg ctaaaaatgt ttgttccaaa gtacatagag actatttata
      841 aagattatgg tgactatata tccaaaatag ttatagaagg tcatacagat gatgtgggaa
      901 gttatatata caacttagat ttatcgcaaa gaagagcata tagcgttgct aaatttatag
      961 tgggagatga aataggtgac tataaataca aagataaagt gactaagcat attattgcaa
     1021 taggaaggtc aaaagctgaa ctcatcaaaa atggtgacaa tagcgtgaat agagatgcat
     1081 ctagaagagt agaactaaaa tacgaaataa atattaatca aaataaataa ttaaattttt
     1141 ataaaaaaaa agaaggcagt tggtgtgagc tttaagcctt ctttaaggaa aaagtttatt
     1201 tatttttgtt gatacttaac atattatagt ttttagaagc agtttgcaat atattaaacg
     1261 taaatggtcg tttaaaatgc ataactgga
//
LOCUS       DAYAOY010000226         1203 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYAOY010000226 DAYAOY010000000
VERSION     DAYAOY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.238"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(127..837)
                     /locus_tag="ACGI6B_004057"
     CDS             complement(127..837)
                     /locus_tag="ACGI6B_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9753425.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          427 a          160 c          122 g          494 t
ORIGIN      
        1 ttttgaaaga ttgaaattct agaaattaag cttcaaaaaa ttaaatttga aaaaacaaaa
       61 ctctaaaaac taagctttaa aaagttaagt ttcaaaaata aaattctaga gattgaactt
      121 caaaaattaa gattctaaca tataccttgt aaattttcct acaacctctt gatatttttt
      181 acggcttata ggaatttttt cattctgaac tttggcataa gtttgagtta tactatctac
      241 atggtttaga ttgacaatat acctttgatg cgtacgtata aaattagagg gaagaacagt
      301 ttccatatca tttaattttc cgtagtattc taatgtttta tcaaaggtaa ctacagttaa
      361 ttttcttagc tcacttttaa agtagaggat atcttttgta ttaattttac aaagattagt
      421 tccaatctga actgtaataa agttttcttt ccattgttct aattttttta gtgcttcttt
      481 taatacttta catatttttt cagttttata aggttttaag atgtagttca aagctcctac
      541 atcatatcca tgaaatacat agtcagaaaa acctgtgaca aagatgatta tagtatcttt
      601 tgaatgtttt cgtatagttt ttgctatgtc aacgccatta ctttttccaa gttcaatatc
      661 taaaaaaata atatctatgt aataattttc gtgttctaga ttgtatacta gactttctcc
      721 atttgaatat tcttttattt ggtaggggta gccccacaaa tccatttctc ttgtaatgat
      781 tttattcagc atgtttcttt gttgtttttc atcatcacaa atacaaatcg aagtcaattt
      841 tttatctcct ttcaagtttt atatttatat agttaagtat ataatatatt ttgctaccta
      901 gatatataaa atgaaataac aattatatcc agatagcaaa ataaaatttt ttatattagt
      961 atacttacac ttaaaactat tctattaaac ttattctgaa gtgctaatga ttttcttatt
     1021 aaaaaatcta acaatctttc ttattataaa atacaaatgc ttttattatt ataaaaagta
     1081 atgattttat tactttaaaa atctaataac ctttcttatt ataaaacacc aatgatttta
     1141 ttactttaaa aatctaataa ccttttttat tataaaacac caatgatttt attactttaa
     1201 aaa
//
LOCUS       DAYAOY010000227         1173 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYAOY010000227 DAYAOY010000000
VERSION     DAYAOY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.239"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1133
                     /locus_tag="ACGI6B_004058"
     CDS             <1..1133
                     /locus_tag="ACGI6B_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9753426.1"
                     /translation="IIISKADRVYLYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVI
                     NNQYTFDLRNDSVLFDDLVLHFKSILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAI
                     EKVFKNSIYSLTEDEIGYVSLHIGAGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQL
                     NKVFHDKINILQCLSFNQFLASELSDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENI
                     SKSITNNSHIYSNLLDNFFDKNLFIVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESV
                     FYRESLSSTNIDDFLAIPHPMELSSIRTKICISILNEPIYWSENSTVKLIMMLAINKD
                     DYIKINSIYDILLKIIHDNDIRDSISNCNDFDNFLSIIKSIV"
BASE COUNT          450 a          127 c          154 g          442 t
ORIGIN      
        1 ctataattat ttcaaaggca gacagagttt atctatataa tcattttata actaaaagtt
       61 ctcttttgga taacgtttcc aatagggtag atactaaaat tatagagtat gttgaagaaa
      121 ttttagaagt aatcaacaat caatatactt ttgaccttag aaatgatagt gtattatttg
      181 atgatttagt actgcacttt aaatcaattc tcaattctaa atcttataat ttaaataagg
      241 tgaatcccct tataaataca ataaagagta attatccttt agcatttgaa ataactttaa
      301 atgctataga aaaagttttt aaaaatagta tttactcttt aacagaagat gaaattggat
      361 atgtatccct gcatataggt gctggaatag agagattttt tcaaagtaat attaagtgta
      421 aaaacgtagt attagtatgt ggttcagggt atggttcaag taggttatta gaggttcaat
      481 taaacaaagt ttttcatgat aaaataaata tacttcaatg tctatcattt aatcaatttt
      541 tagctagtga attgtcagat gtagatataa taatatcgac aattccttta aatcatgata
      601 gtattcctat tgtattagtg gatttaaaac tattaaaaaa agatatagag aatatatcta
      661 agtcaatcac taataattcc catatttata gtaatctttt ggacaatttc tttgataaaa
      721 atttatttat tgttaatcct aaaatcaaag ataaagatga gcttataaag ttgatgtgca
      781 acaaattaac tcaaagtgaa attgtatttc catcatttgc tgaatctgta ttttacagag
      841 aaagtttatc ttctaccaat atagatgatt ttttagctat acctcatcct atggaattaa
      901 gctctataag aactaagatt tgtatttcca tattgaatga accaatatat tggagtgaaa
      961 attctactgt taaattaatc atgatgttgg ctataaataa agatgattat ataaaaataa
     1021 actccattta tgatatcctt ttaaagataa ttcatgacaa tgatattaga gatagtatat
     1081 ctaattgtaa tgattttgat aattttcttt ccataatcaa gtctatagta tagattttta
     1141 tattacataa gttttaaaaa ctttaataaa ata
//
LOCUS       DAYAOY010000228         1141 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYAOY010000228 DAYAOY010000000
VERSION     DAYAOY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.240"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1001
                     /locus_tag="ACGI6B_004059"
     CDS             <1..1001
                     /locus_tag="ACGI6B_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9753427.1"
                     /translation="PLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLITVS
                     TISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFSLF
                     LKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNIYL
                     EKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLIYI
                     DLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNENTT
                     KEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN"
BASE COUNT          469 a          133 c          128 g          411 t
ORIGIN      
        1 taccactata ttatatattg ttttacatag taagtcataa attgctctta agcagaaaaa
       61 gttctactaa caaccatttt attcccaata tggcagtaat aagtttttgt attttacacc
      121 ttattactgt aagtacaata tcacttgtta aaaatacaag tccagatata atatttgcaa
      181 atatgaccta taagttaatt agtattttaa tagcttctat tttagatttt atcttatggt
      241 actctatgaa taaaataaaa gagcatattt atataataac tcaaaatcaa atagagttca
      301 gtctattttt aaaatttatc tggttcactt taggatatat attatttgat agtatacaaa
      361 ttacatttgc tccaccaagt tctctaactt cctactttat attgggaagt aacatattat
      421 taatcttaca actaattata ttcatcaatc atatatattc cattgaaaaa aatatttatt
      481 tagaaaaaga acataataga ctaaaaatga taaaacaaga acaattacta aaagtaaatt
      541 tattaaagaa aaaagctaac attgatgaat taacatcagc atataatcga aaatttgctt
      601 ttgaaaaaat acagcattac attgataaca atttattatt ttcattgata tatatcgatt
      661 tggataaatt aaagacgata aatgatacat atggacattt agctggggat aactatatta
      721 ttaacttctc aagtatagtt attgaaaact taggaataga tgacatattt gctagaatag
      781 gaggagatga gtttatagtt attatgccta aatctaatga aaacaccaca aaagaattta
      841 tagagaaaat aagtaaaatt atggataata cgatatcttt tagctatggt attgtagaag
      901 tttctagcga aaacaaaatg actgtagaag aaattattaa tattgctgat attagaatgt
      961 acactaataa acaaaataat cataaaagga acaataacta aaatgaatat ttacatgagc
     1021 ttacttgtag tctcactatg ttttttatct tattatctct atcattataa tttttttaaa
     1081 tatctggaca taatgattaa taactctaaa aataaataca cagtatatat tatatattct
     1141 a
//
LOCUS       DAYAOY010000229         1120 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYAOY010000229 DAYAOY010000000
VERSION     DAYAOY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.241"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            138..1094
                     /locus_tag="ACGI6B_004060"
     CDS             138..1094
                     /locus_tag="ACGI6B_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFG9753428.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          465 a           90 c          162 g          403 t
ORIGIN      
        1 aataaatgta tttaacactt accttataat aaatttaata taaatattaa ataataatta
       61 ttaaaatagt gtaaaataat tacagtaagg attataaaaa atttttagaa tgtaaattga
      121 aatttaggag tagatgtatg tatatactga atttaaatac aagagagact attgaagatt
      181 ttagagataa attttatgta gcagaaaatt cttatcttat attaagtgct ccaaaaaatt
      241 taaaattgct taaggaaact ttggatatag atgaaataac ttttaatgat tgtcttaaat
      301 ttgatgaaat aaccaaactt gatttatttg ataattacga ttttttaagc ctaaatacat
      361 ttgaactaag agatggagaa gctgttatag aagaggtaaa tatgtattta tcagataatt
      421 ttatattggt tgtagtgaat gaagaacact ttttatttga atttgtaaaa aatattattt
      481 tgaagaactc tcaacttgaa aaaaatccag ttattaatct atttaagata aactacttga
      541 ttcttagaga agttataaaa aatggttttg agtctttgga aaaagttgaa gagttaattc
      601 ttcaaataga agatgaaatg atggacaaca ttaataagaa tcatgtatct agaataagtg
      661 atgtaagagg attaacaaga attatagtta agaatactag acctcttttg tatattgggg
      721 atagaatagt taaggaaaat ataagatatt taaaatattc aaatgtaaaa aaatataatt
      781 tagaaaattt tcaaggtata gattttggaa ttgacaaatt atacaatttc gcattgtcaa
      841 cacgagagtt agcagataaa ttattagata tttattcttc cagagtaggt gagaaaacaa
      901 ataacttaat tacaaaatta accttgttaa cagccatatc agcaccactt acaattataa
      961 ctggtatata tggaatgaac tttagatata tgccagaatt aaactggatt tatggatatc
     1021 cagcaacttt attttttatg ctatgtataa tttttgttgg aattataata tttaaaataa
     1081 aaaaattatt gtaaattaca gatgatgttt aattgatttt
//
LOCUS       DAYAOY010000230         1095 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.242, whole genome shotgun sequence.
ACCESSION   DAYAOY010000230 DAYAOY010000000
VERSION     DAYAOY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.242"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(370..837)
                     /locus_tag="ACGI6B_004061"
     CDS             complement(370..837)
                     /locus_tag="ACGI6B_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9753429.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          426 a          168 c           70 g          431 t
ORIGIN      
        1 gattataata atatttattt atatttctga tttaaacatt attttacttg aaatgtttat
       61 taaataaatg aattaaaact aaactttcat tatattttta atttattcaa ttaaatttta
      121 gcatagttat ataaaatagt atatataatt atacaagata atagtaaata taactatctt
      181 ttatatattt tttctaaatt taaactttag tataaatata taagaaatat cataatattt
      241 ttaaaaactc aaataaagct aaacaagtat tatattcttt attttagaaa aataaaaact
      301 gtaagcaaat ttgttttcac acaagtttac ttacagcctt cacctcaatt ttactttaat
      361 ataatccatc tattctaaaa ctgattcaat atattcgaca gttacatcca ttcttttagc
      421 tacctcatct attgaaagac cactatctaa aaaacgtcta cacacagatg acatagtttc
      481 tccaacctca ataatatgcc catctaaatc ataaaatcta attgctcttt gaccccacct
      541 atgctctata gctagatgaa cataatctat atctttcatt gtactcaatc tttcaacaaa
      601 agtatcaaaa ttatcttcct caaaatataa ctcagcatca tttccattga acttaacttc
      661 actgtcactc ttattaataa attctaacca aagctttttt tcttgtaatg atatattacc
      721 ttctaataca acattttctc caaaatcaca gattaccttt aaccccaaaa catcataata
      781 aaactttttt gatttctcaa tatcttcaac tactactaaa acatttaaaa acttcatatt
      841 acctccttaa tatacacata ctttatttat aatatataat tagaattaaa ctataaaggt
      901 ttcaataagg tctagtataa agaaaaaaat tataagtctt attgaaatag ttaagttaat
      961 tttatcatat aatataaatt ttaatcttgt aaaaaacaga catgttcata cttttaggtt
     1021 taaatctata tgtattctta ttaattttgc taaaactgtt atttttgtag actattttag
     1081 attactgaaa tatct
//
LOCUS       DAYAOY010000231         1074 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.243, whole genome shotgun sequence.
ACCESSION   DAYAOY010000231 DAYAOY010000000
VERSION     DAYAOY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.243"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..1071
                     /locus_tag="ACGI6B_004062"
     CDS             <1..1071
                     /locus_tag="ACGI6B_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFG9753430.1"
                     /translation="YIVNLSKEYYFEHKQWIKKNDIQKLIKGKYNLHSQTIQAISDKF
                     DANRKTISELRKKGNTKAKYPYKTKKFYIIPFKASAINRNAKGNLKLSMSKGRYLELD
                     FNVENIKTAEIVWRNGYYLYYTFDNELNGVVAKGTNTVGVDLGEIHSIASVTNKGVGL
                     ILSNREGRSIKQFRNKMYAYISKRLSKCKKGSRQSKKLWRLKNKIRSKTDNQLMNLYH
                     QTTRKFIDFCVEQKVCEIVLGDIKGVEKDTKKKKRLNRVNRQKISQMEYGRIKDYIKY
                     KAKEQGIEVKLVKENYTSQTCPKCSKKHKPTGRTYSCTCGYETHRDIVGAWNILNKKH
                     KYGLVDFRINHKQPINLKVSTV"
BASE COUNT          475 a          110 c          185 g          304 t
ORIGIN      
        1 tacatagtta acttatctaa agaatactat tttgaacata aacaatggat taaaaagaat
       61 gatatacaaa agttaatcaa aggtaagtac aatcttcata gtcaaactat tcaagctata
      121 tcagataaat ttgatgcaaa tagaaagact atatcagaat taagaaaaaa aggtaatact
      181 aaagcaaaat atccatacaa gactaagaaa ttttatataa taccttttaa agctagtgct
      241 attaatagaa atgctaaagg caacttaaaa ttatcaatgt caaaaggcag gtatttagaa
      301 ttagatttta atgttgagaa tataaaaact gctgaaatag tatggcgaaa tggatattat
      361 ttatactaca cttttgacaa tgaattaaat ggagttgtag ccaaaggcac taataccgta
      421 ggtgtagact taggggaaat acattcgata gctagtgtaa caaataaagg tgtagggctt
      481 attctatcta atagagaagg cagaagtatt aaacaattta gaaataagat gtatgcttat
      541 atatctaaaa gattaagtaa atgtaaaaaa ggtagtagac aatcaaagaa gttatggaga
      601 ttaaaaaata agataagaag taaaacagat aatcagttaa tgaatttata tcatcaaaca
      661 actagaaagt ttatagactt ttgtgtagag cagaaggtat gtgaaatagt tcttggggat
      721 ataaaagggg ttgaaaaaga tactaagaaa aagaaaagat taaacagagt taatagacag
      781 aaaatatctc aaatggagta tggtcgaata aaggactata taaaatataa agcaaaagag
      841 caaggtattg aagttaagtt agttaaagaa aactacactt ctcaaacttg cccaaagtgt
      901 tctaaaaaac acaaaccaac tggtagaact tatagttgta cttgtggtta tgaaactcat
      961 agagatatag ttggtgcttg gaatatttta aataaaaagc ataaatatgg tttagtagat
     1021 tttagaataa atcataagca accaataaat ttaaaagtat ctaccgtttg agcc
//
LOCUS       DAYAOY010000232         1027 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.244, whole genome shotgun sequence.
ACCESSION   DAYAOY010000232 DAYAOY010000000
VERSION     DAYAOY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.244"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(124..615)
                     /locus_tag="ACGI6B_004063"
     CDS             complement(124..615)
                     /locus_tag="ACGI6B_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFG9753431.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          384 a          150 c           95 g          398 t
ORIGIN      
        1 agatttaaat ttaacttata tattttaaaa ggctaatata atatgtattt tcttctatac
       61 aatcaaaagg cttcatatct aagatatgtg atgacactaa tattcacaat tttttagtaa
      121 ttatcacatt ataaaaaata ttacagctag tccatcatca gatgcttctt gtataaactt
      181 tttaagctca attagatttg cccatacata ttcaaaaaaa tcatcttcac tctcatcatt
      241 cataacagga taaatattat tttctaacat ctcattaata ttaaatcttt ttctaaatgc
      301 ttgttcttcc aaactgttca ttgcttttga tagttttttt acatcatttg ctgtaatcaa
      361 catgacagga aattctccat caatttccat aagtatgttt ccacttagta ctaatttgct
      421 aaatatatta tcatcatctc caccaaaagg acaacctgtc aaagtaaagt gaatggcatg
      481 ccatgcttta tcaatattga tagtactatt ttcgttggca tcttcaaaaa caaaatcttg
      541 taagttttct tctgcttgct gtaatttaaa aacattctct tcacttattt tagtataaca
      601 tcctatcatc cccataatta cattcctcct aagtagcgtt tttttaattg taacttataa
      661 ttaaacttag ttacaataaa atatattgag aaaagtatta tttcatctta aatttaaata
      721 cctatattat caactaaaat tctgtgttta tagcgtaagt attattattt tttcctaaaa
      781 attaaatgaa tcagaagttc tataggcaat attgactata aataagaact attactttta
      841 aaaaaaaatt taagaaatta tactgtagaa tcatgctcta cttattagct ttaaataaaa
      901 aataaatcat gttctcaaat taaataataa ataacaataa atttaaattt taaactataa
      961 gagtttctga agtgttaata agcataccaa taattatatg tgtaagaatt tgatttaata
     1021 gatatga
//
LOCUS       DAYAOY010000233         1021 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYAOY010000233 DAYAOY010000000
VERSION     DAYAOY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.245"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(298..786)
                     /locus_tag="ACGI6B_004064"
     CDS             complement(298..786)
                     /locus_tag="ACGI6B_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753432.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKIMDMLYPRIKENELPLF
                     KN"
BASE COUNT          303 a          174 c          116 g          428 t
ORIGIN      
        1 cgatttttag acttctgaat taagatacaa aatataaaat tttgccctca atttctctac
       61 ttttagtgta tacgttgatt aatatttgaa catagttaga attgaactaa cagcgtcctc
      121 acgccctgcc tagtctgttc gtagtgacta gggcaatccc ttatacccta gtcaaatatt
      181 aagttttgag agggaaatct ttatttccac aatactatta tctcatgctt ttttaatcaa
      241 aaaggggaga aagtagggaa taaagtggga atttctgggg aaaaactggg gaattttcta
      301 atttttaaat aatggtagtt cattttcctt aattcttgga taaagcatat ccataatttt
      361 atacactaat ctttccctca cacatctaca tgtttttcta tctgagttca tctctaagga
      421 tatataaacc atactatttt tcattctgct attgtaaaac agtttaaaaa aatgttcttc
      481 tcttatatct aagcatgtaa gtgcattttc tattttcttc ttttcaattt ccttatcttt
      541 tttcagtttt ttcaatctag taatatctct ttcttttttt ataatctcat tctccacact
      601 tgaattaaaa gcatatgttg gacttacttt ttcatcatat ccaacagcct tacacccaaa
      661 tatctcattt tctctacttt ctatatctaa ttcaagattt ttaatttctg cacttaaaaa
      721 tttataatga tgtagtctac cttctacttt tttaaatagt tcttttttat tgatattatt
      781 atccatactt ccacactcct gtttatgtta taataatctt ggataaaagc tttatatttt
      841 tgacaagtgg agtgtgaaag cactcctttt ttctttttat taacagaaat tatctttttc
      901 aaagaaacta atttgatttg tttttctctc agattttata attctgattg attcatctat
      961 taagtttaat gaattaagta atacatcttt cgggatatcc tcccatttgt cagcacctaa
     1021 t
//
LOCUS       DAYAOY010000234          964 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYAOY010000234 DAYAOY010000000
VERSION     DAYAOY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.247"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            178..561
                     /locus_tag="ACGI6B_004065"
     CDS             178..561
                     /locus_tag="ACGI6B_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9753433.1"
                     /translation="MLNRKLPDAELKIMKYIWNTGYKTVISKDVADEIEKTYGWKHTT
                     TITLLARLTKKGFLISQRIGKHVHYTVLVKEREYLKLEGKRIFGGLHNNPLSELISKL
                     HDGEEITEEKIIEIGNWIKSWKDED"
BASE COUNT          421 a           84 c          143 g          316 t
ORIGIN      
        1 gtttgaaagt tgaataattt tatagatatg aaagttattt ttcttctaag aaaacaaact
       61 aaaacagtag aatatatggt aaaaattacc tatagttttt gtcctaaaaa gctcctatta
      121 ttaatttaag ctagataaat ttgaagaagt gacataaatt gaaagaagga atatattatg
      181 ttgaatagaa aactaccaga tgcagaatta aaaattatga aatacatatg gaatactggt
      241 tataaaactg taatatcaaa agatgttgca gatgaaatag aaaaaacata tggttggaag
      301 catacaacta caattacact tttagctaga ttaaccaaaa aaggtttttt aatatcgcag
      361 agaattggga aacatgtaca ctatacagta ttagtaaaag aaagagagta tctaaaatta
      421 gaaggtaaaa gaatttttgg aggtttacat aacaatccct tgtcagaact aatttcaaaa
      481 ttgcatgatg gagaagaaat aactgaagaa aaaataatag aaataggaaa ttggataaaa
      541 agctggaaag atgaagatta ataaataaaa ggtagctact aatataagtg ctaccttttt
      601 tcttgtggat aatattgggg ataagttgtg gaaaacttat gtaaaatttg tggataaaat
      661 attttactga catttgactg acataaatat caaaacttga ttttaaagta ttgaaatttc
      721 aattattaat catataaaaa tagaaaagtt attattgtaa taaagaataa taaatatatt
      781 aatatcatta ataattgtta taaatgtgaa atactaacaa tatctaaact aaattattat
      841 aaaatagctg actgtaattt aattgataaa gcctattctt ctagactttg ccagttaaat
      901 tacagtcagt tttattatat aaaaaaatgc gtaatggaac tattatttta tagattatat
      961 ttga
//
LOCUS       DAYAOY010000235          880 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYAOY010000235 DAYAOY010000000
VERSION     DAYAOY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.249"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..180
                     /locus_tag="ACGI6B_004066"
     CDS             <1..180
                     /locus_tag="ACGI6B_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9753434.1"
                     /translation="INRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNT
                     KLLTNCIIKDIKNQS"
     gene            373..633
                     /locus_tag="ACGI6B_004067"
     CDS             373..633
                     /locus_tag="ACGI6B_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFG9753435.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT          403 a           68 c          139 g          270 t
ORIGIN      
        1 ataaacagga taaaattata tgattttaac aaggttatag atgttgtaaa tacaaatatt
       61 gattctaata gaaatttagc ttctaatttt gaagttagac ctaaaattag tattaaaaag
      121 aacaaaaaca caaaattatt gacaaattgt attataaaag acataaaaaa tcaaagttaa
      181 ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa gtgtatatta gtagtataat
      241 atttttgtag cactagtggg aacaaaaaat gaccatagga caaaaaagta ccacttagta
      301 ccattaaagt aaaaaacagg aaaacaattt taaaaaatat aaaatgtaaa gtttatagga
      361 ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg caagtggatt acatgctaga
      421 ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat caacagtaga ggttgtagca
      481 aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa tgagtttagg tttagctcaa
      541 ggagaagaat taaagttagt tgtaaatgga gaagatgaag aagtagcttt agctgcaatg
      601 gttgaattag ttgaaagtgg atttggagaa tagatataat aattttttaa aaatttgata
      661 tttagaatct aatattactt aaagtaaagt aacagagaag tattaaacat atacagacca
      721 acatttgtag atatttaatt tctttgttac tttcatgttt atttaatcag aagattaaga
      781 ctattgttaa ttgggtataa aaatataaat attttagaaa atgaaataag atatttttaa
      841 tcataaattc agagtaaaaa tacagtaaca aatagtttat
//
LOCUS       DAYAOY010000236          862 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYAOY010000236 DAYAOY010000000
VERSION     DAYAOY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.250"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            130..780
                     /locus_tag="ACGI6B_004068"
     CDS             130..780
                     /locus_tag="ACGI6B_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFG9753436.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
BASE COUNT          376 a           78 c          126 g          282 t
ORIGIN      
        1 attctgcgtc agtagaatga atttgtcaag tttaatttag ttgtaatttt atcttttatt
       61 aaaaataata taaattaaaa catataaata aaatatttta tacaatatat tgtaaaggag
      121 gtaactttga tgaaagcaga aaaaacaagt atatatccaa ttattaaaaa taatttaaac
      181 aaaatatcaa ttgttgaata taaaaagggt caaagatttg tagcatcaga taaagactta
      241 caagaagtgt tctttataat agagggagtt gcattagtgg agtgtactac tagaggagga
      301 aataaatttc ttgttgacat tgtacctgaa aatgaattta taggcaaaat tagttacata
      361 tatgaacata atttgaaatg tgatatattt gcaaagacca atataaaatt atttcgattt
      421 gaaaaaaatg tatttgaaga gttttatctc aaaccagatt ttattgcact atttcacaga
      481 aaatgtacaa gaagaatata tgaattatat aaaaccagaa tggtaagaga actattttct
      541 tgcacagaag ttattgccta ttgtatatca aataaacaag aaaataatgt ctgcaatata
      601 aaagctattc gtaatttcag tgaaatctat tcaattagta gaaaaagtag ataccattct
      661 ctgctaaagt taactgaaaa ggagatagta aaaaaaatgg gtaatacata tgaaatttta
      721 aactatgatg aattacataa attggcattt gaagtaaaag aatttttgga agatgagtaa
      781 aaattgttac ttaatcaaaa taaagtataa taatttctgt aaaaaataga aatagtctca
      841 ctgaaaatca aaaatacttt ag
//
LOCUS       DAYAOY010000237          835 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.251, whole genome shotgun sequence.
ACCESSION   DAYAOY010000237 DAYAOY010000000
VERSION     DAYAOY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.251"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            285..755
                     /locus_tag="ACGI6B_004069"
     CDS             285..755
                     /locus_tag="ACGI6B_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFG9753437.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
BASE COUNT          382 a           79 c           90 g          284 t
ORIGIN      
        1 attaaattag atgcatagaa taactttttt aaacattgaa aataaattaa actctaaaca
       61 catttaaata taaatttata tatcattttg acttggttaa acaatttcat acttatcgat
      121 ataaaaatag gttataatat aaatgttcat tatcttataa cctattttat ataatttatt
      181 cctaaaacat gtgaatgttt tttatgatag tttgaagaaa ttataaaatc actttataat
      241 tgaatattat atataaatta atttaaatat agaaaggaaa atatatgtac aacaatatca
      301 aaaatatttt aaaagaaaat catataacgt ttgaagaata tgaccatgaa cctattttat
      361 catttgaaaa agctaaagaa ataagagaaa aattacaata tactggagta gaaagtaaaa
      421 gtttatttat aaaagataga tctggaaatt actatatatt tgtaaccaca gaaggaaaaa
      481 aattagatcc aaaaactata aaaaatgttt taggtggaaa agtatctata tgttcaaaag
      541 aagaattaac acaaaaaaca caatgtttgc ctggatgtgt atctcctttt ggatatgaga
      601 aaaatatctc tcttgtaata gatcaagata tatttaacca tgataaaata atcttttcac
      661 cagggatacc agaaaagaca tttattatta cagctagtgg atttaaaaaa gtaataaaca
      721 atataaacaa tacaacttat aaatatatat attaatgaat tttaatcata attgtaaatt
      781 agagaaaata acattagaaa tcatgcttaa ctatagtgta aataaattat attca
//
LOCUS       DAYAOY010000238          789 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYAOY010000238 DAYAOY010000000
VERSION     DAYAOY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.253"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            134..640
                     /locus_tag="ACGI6B_004070"
     CDS             134..640
                     /locus_tag="ACGI6B_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HFG9753438.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          321 a           76 c          134 g          258 t
ORIGIN      
        1 agtaattgat ttgttgtatt ataatttaca tgaattatgt aggacaatca aaagtcagta
       61 ttataagagt acatatattc aaatttttaa caattaattt atataattaa aaaatgaagg
      121 gtaggatgaa gtcatgggtt ttagagaagt aaaaattgag gaattacagt ttaatccatt
      181 tactaagatt ggcaaagaat ggttacttat aacagctggt gattcagaaa agtttaacac
      241 aatgactgct agctggggtg gtgttggagt ctattggggt aaaaatgttg ttacaactta
      301 cataagacca cagagatata caaaagaatt tgtagatagt aatgatacat ttacagttgc
      361 cttctttgat gaaacgtata gagaggcttt aaatatttgt ggtactattt ctggtagaga
      421 tataaataaa atagaaaaag caggacttac accatatttt gtggatgata cagtagcttt
      481 tgaagaagct aatatgatta tagtatgtaa aaagctatat catggtaata tgccaccaga
      541 aaactttgat gctaaagaaa atgataaaaa atggtatcca gagaaagact accatactat
      601 gtacatttca gaaattataa aagttcttgt aaaagaataa ttataataaa aaatatatga
      661 aaagaactta ttataagtaa atgactgtgc tttcttatct gatatctaag tagatgctag
      721 taactaaata aatattaatt ttaaataaat actattataa aaaagatgaa ctatatttac
      781 tatagttca
//
LOCUS       DAYAOY010000239          787 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYAOY010000239 DAYAOY010000000
VERSION     DAYAOY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.254"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..>787)
                     /locus_tag="ACGI6B_004071"
     rRNA            complement(<1..>787)
                     /locus_tag="ACGI6B_004071"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAYAOY010000240          754 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYAOY010000240 DAYAOY010000000
VERSION     DAYAOY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.255"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..261
                     /locus_tag="ACGI6B_004072"
     CDS             <1..261
                     /locus_tag="ACGI6B_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753439.1"
                     /translation="HLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNYMNF
                     GITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            273..>754
                     /locus_tag="ACGI6B_004073"
     CDS             273..>754
                     /locus_tag="ACGI6B_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9753440.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     "
BASE COUNT          299 a           92 c          148 g          215 t
ORIGIN      
        1 catcttgaaa gtgatgctag aaatcaagat tggtttgagg aagttcattc ttttgtaagt
       61 aatattaagc catgtaatat gactttagat tacactagag tgcttataag caaagacaat
      121 tatatgaatt ttggtataac aaccctaatg ggtcaagaaa taactatata cccttggagt
      181 ccaccagata tagaaactta tggagaaatt gatgtattaa ctggcaatgg agttggatac
      241 caagagataa caatatttta ggaggtgata tattggctat agataaaagt tattacacta
      301 taattacaga tgtaggaaaa gcaaagatag caaatgcaag tgtcacaggt aataaagtgg
      361 gatttgtaaa aattcaactt ggtgatggag gagggagtga atatactcca actgagagtc
      421 agacagctct caaaaacgtg gtatgggaag gcaatattgg aaatacaact acagatgaaa
      481 ctgcaccaaa ttgtataata ttagagagtt taataccatc aagtgtaggc ggatttatga
      541 taagagaaat aggatattta gatgatgaaa ataatttaat tgccatttct aaatacaaag
      601 agtgttataa accttctata gaacaaggtg cagtggtaga catgaaggtt aaaactgtgc
      661 ttattgtatc taatgtaaat aatatagaac ttaaaattga cccaacaata atctttgcaa
      721 cactcaaaga tatacaagac ttagaaacta aaat
//
LOCUS       DAYAOY010000241          753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYAOY010000241 DAYAOY010000000
VERSION     DAYAOY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.256"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..>753
                     /locus_tag="ACGI6B_004074"
     rRNA            <1..>753
                     /locus_tag="ACGI6B_004074"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAYAOY010000242          751 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYAOY010000242 DAYAOY010000000
VERSION     DAYAOY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.257"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            376..474
                     /locus_tag="ACGI6B_004075"
     CDS             376..474
                     /locus_tag="ACGI6B_004075"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753441.1"
                     /translation="MFILKRLCKLVSINKSFSEEIPKNLKNTYMCI"
BASE COUNT          307 a           56 c           81 g          307 t
ORIGIN      
        1 gataatatat tactaaaaaa ttttgtaatc ataaaatatc atctccttta tataaagtat
       61 attattgatt ataatataca atttattgaa tttcaataat aaattattga tataatatat
      121 aaaaccattg taatatacac taaaaataag aatgtataag gtggtaaatt tatataaaaa
      181 agaatatgag ttgtaaaata ttaaataata ttgtactaat tggaataatt ttaactttat
      241 tatagattat tttaatacca ttagtactga tggtgtattt taaaattggt ataagcatta
      301 caaatacgaa tatgccaact gtaatatgta atataagtag ctatatacat atgtgctgtc
      361 ccatatattg tatcattgtt tatattaaaa agattatgta aattggtatc aatcaataaa
      421 tcattttcag aagaaatacc taaaaactta aaaaatacct atatgtgtat ttagttagat
      481 ttgagatttt tacatttaat tttatagtga caatattata ttatttttat gatgtctatt
      541 tatatgcaat tactataata tcagaactta tagtatcatt tgtttcatta tcaataaggt
      601 tttttacttt agttttgtca attttattaa aaaaagttat tgatataaaa gataagaagg
      661 ataaaactat atgacggatt tcaaacataa gagtggatat ttatataata gatattttat
      721 agttaatttt attagatttt atttaatact g
//
LOCUS       DAYAOY010000243          671 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYAOY010000243 DAYAOY010000000
VERSION     DAYAOY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.259"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            125..>671
                     /locus_tag="ACGI6B_004076"
     CDS             125..>671
                     /locus_tag="ACGI6B_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9753442.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNV"
BASE COUNT          251 a           84 c          118 g          218 t
ORIGIN      
        1 ataaagctga aaattattat taagtttttg tagaaatatt ataagcatag atacattttt
       61 gtaacaacta caataaattt ttaatactat atactattcg tatgtcttat gggaggtatt
      121 tattatgttt aaaaaaatag tcgcaatagc aagctctatc attgtaagta caatatgtct
      181 aactggatgt gattttaact ctaatgacaa aaaaggtgaa tcttcttcgt ctaaaaatct
      241 ggataaagta actatagcag aagttactca ctctgttttt tatgctccac agtatgctgc
      301 tattacgaaa ggattctttg aagatgaagg tataaagatt gaccttataa atacccaagg
      361 tgcagataaa actatggcag ctttgatttc aggtgaagca aacgtaggtt taatgggtcc
      421 tgaagcatct atatatgtat ataatcaaaa caaaaacgat tatgcagtga actttgctca
      481 attgacaaaa agagatggta gttttctagt tgctaggaaa aaaatgccta atttctctta
      541 caatgattta aaaggaaccg aaatattagg tggtagaaaa ggtggagttc cactaatgac
      601 ttttgaatat gttctaaaag aaaaaggtct tactattgga gaaaatctaa aagcaggcaa
      661 tgttaatgta a
//
LOCUS       DAYAOY010000244          664 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYAOY010000244 DAYAOY010000000
VERSION     DAYAOY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.260"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..>664)
                     /locus_tag="ACGI6B_004077"
     rRNA            complement(<1..>664)
                     /locus_tag="ACGI6B_004077"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAYAOY010000245          577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYAOY010000245 DAYAOY010000000
VERSION     DAYAOY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.264"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..>577
                     /locus_tag="ACGI6B_004078"
     rRNA            <1..>577
                     /locus_tag="ACGI6B_004078"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAYAOY010000246          521 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYAOY010000246 DAYAOY010000000
VERSION     DAYAOY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.265"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..>521
                     /locus_tag="ACGI6B_004079"
     CDS             <1..>521
                     /locus_tag="ACGI6B_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9753443.1"
                     /translation="DTTKTELTNNLETAKTELNTRIDTENEKQNIKIDQLIAGGSNVA
                     STQTITIDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNTIEI
                     RSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKLSGGLS
                     NIATSVNELITYC"
BASE COUNT          228 a           57 c           92 g          144 t
ORIGIN      
        1 gatacaacta aaacagaatt aacaaataat ttagaaactg ctaaaacaga gttaaacact
       61 agaattgaca cagaaaatga gaaacaaaat attaaaattg accaattaat cgcaggtggt
      121 tctaatgtgg catctactca aacaataaca attgacgatt gggttgagga tgcagaaaat
      181 ggattcaaag caactgtaac acatagtttg ttaacacaga gaatagttgt aaatattata
      241 gatgctacta caaaagaaaa tgtagttaca aactttaaaa ttatagatga taatactata
      301 gaaattagaa gtgaaacaag gtcagaatta aacgtttatg tgataaatgg aaatgcagaa
      361 actcatttta taaatgcaac tgtagatgat aacagagtgt ctgaaatgac tacttattcg
      421 tctaagaaaa tagaggatag attggttaat atagaagaaa aattaagtgg tgggttatct
      481 aacattgcaa caagtgtaaa tgaattgata acttattgtt a
//
LOCUS       DAYAOY010000247          493 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYAOY010000247 DAYAOY010000000
VERSION     DAYAOY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.267"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="ACGI6B_004080"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="ACGI6B_004080"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="ACGI6B_004081"
     tRNA            310..398
                     /locus_tag="ACGI6B_004081"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="ACGI6B_004082"
     tRNA            410..486
                     /locus_tag="ACGI6B_004082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAYAOY010000248          483 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYAOY010000248 DAYAOY010000000
VERSION     DAYAOY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.268"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(298..>483)
                     /locus_tag="ACGI6B_004083"
     CDS             complement(298..>483)
                     /locus_tag="ACGI6B_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753444.1"
                     /translation="KDGTIVPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTT
                     VSSLLGRIAVNIFNFNK"
BASE COUNT          168 a           66 c           52 g          197 t
ORIGIN      
        1 tgattctaag ttaataaaga aatatttaaa aaaatcaaaa aattttctta aataagtaat
       61 aggttttgta aaaatggaaa aatatcaaaa taagactatg atttttaaaa ctatagcctt
      121 atcaataaat ttttatttaa agaacacaca taaatatttt taaacctaat ttatgtgaag
      181 ttttatgtat tttttatata agtagtattc taatcttact catctaatat accaaaaaga
      241 ctgtaagtaa gttcactttt atgcaaactt acccacaatc tcttttacac attaattcta
      301 tttattaaaa ttaaaaatat ttactgctat tctacccaat aaagatgata ctgttgttgt
      361 tctggctggt actgttactt ctatttctcc tgtatctgct gttttaccac ttgatgtgtg
      421 tgtcactgta aatactaatg ttacttttgt ttctgtatct ggtggcacta ttgttccatc
      481 ttt
//
LOCUS       DAYAOY010000249          471 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.272, whole genome shotgun sequence.
ACCESSION   DAYAOY010000249 DAYAOY010000000
VERSION     DAYAOY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.272"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
BASE COUNT          166 a           32 c           67 g          206 t
ORIGIN      
        1 gttaatggtt ttattaaaaa aatatatttg taattcttat gtatagttat atttttgttt
       61 ccaaaagatg ctattttgca tatgtaaatg ttacatagtt cgattattaa tatagtagtt
      121 atgattaaaa atatatggta tttcatagta catcctcttt taaagtgaaa ttgtatttac
      181 ttaaaataga ttagatttta tatattctat tttacagttt ttatttaggt acatgcaaag
      241 agcttattat ggataccttc ttttttattg catgtgtctg tgttatataa aatttaaaaa
      301 gtattatatc ttaatataca gtatgtatga taactatgtg tccaaatata gtaattatgt
      361 atggaaagta taatagttat gtgtataagt atagtaatta catatagcat gtatgatagt
      421 tatgtgtata agtatagtaa ttacatatag catgtatgat aattatgtgt a
//
LOCUS       DAYAOY010000250          456 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYAOY010000250 DAYAOY010000000
VERSION     DAYAOY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.273"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            12..410
                     /gene="tnpA"
                     /locus_tag="ACGI6B_004084"
     CDS             12..410
                     /gene="tnpA"
                     /locus_tag="ACGI6B_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFG9753445.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
BASE COUNT          180 a           52 c           82 g          142 t
ORIGIN      
        1 tgttagtttt tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg
       61 tattttgccc taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag
      121 aaatactacc atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata
      181 aagaacatgt gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa
      241 aaggtttaaa aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta
      301 gtatgcctag catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag
      361 aagtagttaa acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca
      421 tagagtgaaa ttaaacctaa ctagaacaca atttga
//
LOCUS       DAYAOY010000251          417 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYAOY010000251 DAYAOY010000000
VERSION     DAYAOY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.277"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..>417
                     /locus_tag="ACGI6B_004085"
     CDS             <1..>417
                     /locus_tag="ACGI6B_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9753446.1"
                     /translation="DTTKTELNTRIDTENEKQNIKIDQLIAGGSNVSHTHIIEIDDWI
                     LNNETNMYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENIKMYVY
                     LINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFLN"
BASE COUNT          176 a           47 c           67 g          127 t
ORIGIN      
        1 gatacaacta aaacagagtt aaatactaga attgacacag aaaatgagaa acaaaatatt
       61 aaaattgacc aattaatcgc aggtggttca aatgtatctc atacgcatat tatagagatt
      121 gatgattgga ttttaaataa tgaaactaat atgtatgaag taactataaa tcatccacta
      181 ttgactaaaa gaatactaat agctttatat gatgaaattg gtgaagcact tacaccaaac
      241 gctagggcta ttgatgataa tagtattctt gttagaaatg aagaaaatat taaaatgtat
      301 gtatatttga taaatggaaa tgcagaaact cattttataa atgcaactgt ggatgataac
      361 agagtgtctg aaatgactac ttattcatct aagaaaatcg aagatagatt tcttaat
//
LOCUS       DAYAOY010000252          378 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYAOY010000252 DAYAOY010000000
VERSION     DAYAOY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.279"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..235)
                     /locus_tag="ACGI6B_004086"
     CDS             complement(<1..235)
                     /locus_tag="ACGI6B_004086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753447.1"
                     /translation="MDKLYICSSQGVEEVEILEGTKGRFKVGSNSQYLRVINKNVLDV
                     KSASYVASLNRDTAINIWNDEISKEIERLKGFLY"
BASE COUNT          116 a           88 c           26 g          148 t
ORIGIN      
        1 tatataaaaa tccttttaat ctttctattt ctttgcttat ttcatcattc cagatgttta
       61 tagctgtatc tctatttaaa gaagcaacat aactagcact ttttacatca agtacatttt
      121 tattaataac tcttaaatat tgactattac tccccacttt gaatctacct tttgttcctt
      181 ctagtatttc aacttcttct actccttgtg aactgcatat ataaagttta tccattattc
      241 ttctacctcc atataaagcc attctttgaa cccttcggga ctttcataac atatataacc
      301 acaaggttct ccctcatata tacaatactc acaaatacaa tctgtacttc caaaaaaatt
      361 agccattcca tcctcatc
//
LOCUS       DAYAOY010000253          374 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYAOY010000253 DAYAOY010000000
VERSION     DAYAOY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.280"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..372)
                     /locus_tag="ACGI6B_004087"
     rRNA            complement(<1..372)
                     /locus_tag="ACGI6B_004087"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          102 c           63 g          122 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccag
//
LOCUS       DAYAOY010000254          364 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYAOY010000254 DAYAOY010000000
VERSION     DAYAOY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.282"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="ACGI6B_004088"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="ACGI6B_004088"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="ACGI6B_004089"
     tRNA            291..362
                     /locus_tag="ACGI6B_004089"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAYAOY010000255          349 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.283, whole genome shotgun sequence.
ACCESSION   DAYAOY010000255 DAYAOY010000000
VERSION     DAYAOY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.283"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            128..319
                     /locus_tag="ACGI6B_004090"
     CDS             128..319
                     /locus_tag="ACGI6B_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HFG9753448.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT          154 a           22 c           66 g          107 t
ORIGIN      
        1 aatatgtaat actcgcaata ggatataaat ggtatatatg gtaataattt atattaaaaa
       61 aacttttata aagtttatat ttgggtataa aagtattaat tagacattaa ttaggaggaa
      121 cttaaatatg ataagagtat gtccaaactg ttcaaatgtg gatgtaaata agctaaaaac
      181 tttggttgga gaagaaaatg taaagactgg ttgtataggt cagtgtagag catttaaaaa
      241 agaagctgtt ggaataatag acaatgagct tgaaataaag gaaacagaag cagagttttt
      301 tgaagcatgt aaagaataaa ttttaaataa aatataataa atagagcat
//
LOCUS       DAYAOY010000256          346 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYAOY010000256 DAYAOY010000000
VERSION     DAYAOY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.285"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(108..284)
                     /locus_tag="ACGI6B_004091"
     CDS             complement(108..284)
                     /locus_tag="ACGI6B_004091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753449.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
BASE COUNT          143 a           62 c           27 g          114 t
ORIGIN      
        1 aaaaaataaa attatgaata ctagaaatct atcttaatta gctttctaac ataaggctat
       61 ttaaagtaga taaaactaaa aagggttctc tagaaaacca aatctattta attatactta
      121 cttactaata ccatcacttg atgcaaacaa gattctacca cattttctcc gtactctagt
      181 ataaaatcat ctagttgtgt atctataaaa tcacaataat catatgaact accaatataa
      241 gcagtcaaca tatgtgtaca tctatccatt ttatctctaa acatatatat tattatcccc
      301 ctaaaaaact ttcataaaaa caaaaagaat atctcttttt ggcaac
//
LOCUS       DAYAOY010000257          313 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYAOY010000257 DAYAOY010000000
VERSION     DAYAOY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.denovo.290"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..>313
                     /locus_tag="ACGI6B_004092"
     CDS             <1..>313
                     /locus_tag="ACGI6B_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFG9753450.1"
                     /translation="VLKLDVAKELAMVQNNNKGREIRRYFIRLEKLLNRTLSNSQLSQ
                     INTIVNESLLKMEVKHNIQIEQLKKECSEYYRPTSKTKYDISSYIKERLGISKVNEEF
                     EL"
BASE COUNT          144 a           33 c           46 g           90 t
ORIGIN      
        1 gtattgaagc ttgatgtagc aaaagaactt gctatggtac aaaacaataa taaaggaaga
       61 gaaataagaa gatattttat aagattagaa aagttactaa atagaacctt atcaaattca
      121 cagcttagtc aaattaatac tattgtgaat gaatcattac ttaagatgga agttaaacat
      181 aatatccaaa tagagcaact taaaaaagag tgttcagaat attatagacc aacctctaaa
      241 actaaatatg atatatcttc ttatataaaa gaaagactag gtatatcaaa agttaatgaa
      301 gaatttgaat tag
//
LOCUS       DAYAOY010000258         9781 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.131, whole genome shotgun sequence.
ACCESSION   DAYAOY010000258 DAYAOY010000000
VERSION     DAYAOY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.131"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..533
                     /locus_tag="ACGI6B_004093"
     CDS             <1..533
                     /locus_tag="ACGI6B_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753451.1"
                     /translation="YKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENI
                     EDIKFIKDGTKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKI
                     KIEREVNIFEDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNV
                     QYTKNRVIILYYIYNH"
     gene            complement(861..1628)
                     /gene="aroD"
                     /locus_tag="ACGI6B_004094"
     CDS             complement(861..1628)
                     /gene="aroD"
                     /locus_tag="ACGI6B_004094"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9753452.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2300..3136)
                     /locus_tag="ACGI6B_004095"
                     /pseudo
     CDS             complement(2300..3136)
                     /locus_tag="ACGI6B_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3207..3875
                     /locus_tag="ACGI6B_004096"
                     /pseudo
     CDS             3207..3875
                     /locus_tag="ACGI6B_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4002..4622
                     /locus_tag="ACGI6B_004097"
     CDS             4002..4622
                     /locus_tag="ACGI6B_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9753453.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4778..5560
                     /locus_tag="ACGI6B_004098"
     CDS             4778..5560
                     /locus_tag="ACGI6B_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9753454.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            5685..6611
                     /locus_tag="ACGI6B_004099"
     CDS             5685..6611
                     /locus_tag="ACGI6B_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753455.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            6625..7074
                     /locus_tag="ACGI6B_004100"
                     /pseudo
     CDS             6625..7074
                     /locus_tag="ACGI6B_004100"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            7488..7841
                     /locus_tag="ACGI6B_004101"
     CDS             7488..7841
                     /locus_tag="ACGI6B_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9753456.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            7843..8073
                     /locus_tag="ACGI6B_004102"
     CDS             7843..8073
                     /locus_tag="ACGI6B_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9753457.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            8463..8666
                     /locus_tag="ACGI6B_004103"
     CDS             8463..8666
                     /locus_tag="ACGI6B_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9753458.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            8744..9076
                     /locus_tag="ACGI6B_004104"
                     /pseudo
     CDS             8744..9076
                     /locus_tag="ACGI6B_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            9169..>9781
                     /locus_tag="ACGI6B_004105"
     CDS             9169..>9781
                     /locus_tag="ACGI6B_004105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9753459.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSG"
BASE COUNT         3524 a         1451 c         1560 g         3246 t
ORIGIN      
        1 aatataaaaa tggctacatc cctaataaat taaacctttc ttttggatat ggattctctt
       61 cttataaaaa gcaaataatt agagaaactg tagatacttt aagattgaca gaaatttttt
      121 caagcgagaa catagaagat attaaattta taaaagatgg tacaaaaaaa ttagaaatta
      181 gcatagagaa agttgtgaaa tttaaacgtc gaaaaaaaag aaattatgtt tgttgctatt
      241 gccctgatat gtatagagac ataaaactcg acaaagaatc tatcaataag atatacaaca
      301 gaaaaataaa aatagaaaga gaagttaata tttttgaaga tgaggatgtt ataataaaca
      361 agaaagtatt gaagtttcca aagtcttgga caaagaatat gcaaaaatat tggttaagtg
      421 aaaataagta tcccatacat tctactgtaa ttgatgatga tagatataaa tgttgtaatg
      481 tacaatatac aaaaaataga gtgataatat tatattacat atataaccat taatatacat
      541 aattacaaaa aaataatgac aaatatacat aatacaaata tatcttctac tttagataaa
      601 acagttatta tgaaaactta ctatctataa ataatagaaa tgctagtata ccatctataa
      661 tctaatgtct agtaggtttt aatggcatat tttaaatatg ccatagttga tttataagat
      721 tttctatatc actttcaaaa tacacactaa atagttgctt ttcagatata attgcccaat
      781 ttaaatttta ctatctgaaa agttttttat atttaattta tatcttcaaa aataccgtct
      841 aatatttcta tataattaat ttaattaata ctcttatgta atagattaag tactgaattt
      901 aactctttaa atgatatttg ccctggtgct gaaacacttt ttgcagctcc aaaagtcaat
      961 gcagaaccaa atatttcacc acacaatcta cttataacac ccattcctga cattgacatt
     1021 gttattattg gtctatctgc ataaatttta aacatttcat ttgtagcttc aagtagcact
     1081 aacacatctt tttcattttg cggcataaca gctattttgg gtaaatctgc gcccaattcc
     1141 tgcattctac ataaacgaga tacaatctct tctttttttg gagttttatt aaaatcatga
     1201 ttagatataa ttactttcac ttctttttta tgagcaaaat taactacttc atctatgacc
     1261 tcatctccca taaataactc tacatctatt aaatctacta aacctgtatt agaaatttct
     1321 ttattcaaag tagtataata atctcttgat attaattttt ctcccccttc tactacactt
     1381 ctaaatgtga ataataaagg gatatcatgt atataacttc ttaattcata gagaacttcc
     1441 ttaacttctt taatattttc tacattctca aaaaaatcaa ctcgccactc tataatatcc
     1501 aaacatgcat cttttaactc tttagcctct tttataatat cttttttatt tttcccaata
     1561 attggtacac aaattttagg tctaccttct ccaatagtaa tattttttac ttgaactttt
     1621 cttttcatat tctcctccat aatcagaaat tctataggac tttaaattct attttctgca
     1681 caatttaatt atacattaaa aatactatac attataaaaa tactaattta aaaataattt
     1741 ttattctaat tcttaaaaca atcccacata ttttagaaaa ggtactctat aatatacaaa
     1801 cacttctttt ttacctaaat ttattttaac atcctataat tttcacaata ttctttaaat
     1861 tttactcata taaacaatat tattctggat gctaatagaa ttaatctata aatcatagta
     1921 taattatgca gaaataactc gaatttcact acttatgaag agattgaata ttaatgaatc
     1981 taaatcattt gcattactta agagttttga ctaaagttga acaccataca aaagtagcta
     2041 ctcaattgtt tataccccaa ccaagcttaa gtaatgttat atagattctc gaaaaagaat
     2101 ttggtatctt tttatttgaa aaacttatat taaatttgat tatcttttca taagttggta
     2161 aatgtcgata tatcctgtat ctacaaggag tatcggcatt ttctttttgg aagatacttt
     2221 gcataagttg gcatttaacc gtataagaat gccatcaaaa gtagtaaata agtagtaaac
     2281 tctagtcata ctactaggat tataaccgtt ccatttcagc tctcgaagaa ctaaatgaac
     2341 catgtgcata atagccaagc gtcatagtaa tgtttttatg ccccataata tattgcaata
     2401 tattaggact cattcctttg ttagccatat ttgtacagag tgtatgccta aaggtatgtg
     2461 gcgtaatctt tggtagcttg tcctcatggg tcttattata cttcttgact aacgctctta
     2521 acatgctttg ataatgagtt gcttgttttg gtaatccatc ttgtttaaga aataggaagt
     2581 tctggtaacc atcaacaatg attggttctg ctttagcgtt cttacgccgt tccacgatac
     2641 gttcaaatga ttgataagct ctttcagtca ttggaatttc tcgcttaccc tgtgtggtct
     2701 ttggaacttc tacataatag ccaatcttag tatcactcaa caattgatgg tctaccataa
     2761 tggctttatt tttcatatcc aattctaaag tcaaaccgca taattcagaa atgcgtagtc
     2821 ctgtttctaa gaggataaca acatcatcat aatatttaca ataagtctta tcgctcccca
     2881 taaaggataa taagcccatt tcctgttctg gtgttagagc gatttttggt tctgttgtat
     2941 cttccaaaac atcacttaac taaaaattaa atggattctt tctaatacag tcgtcttgaa
     3001 ttgccatgaa aaacgacgct ttcagtgagc gcttataatt gtcaatggat ttataagcac
     3061 acccatcttc actcattcgg atagcccagc ttttagcgtc tgataattta accatatcaa
     3121 tgctttttgc acctagtggg tcattgatag tatctatatt ttgatttata atttatacat
     3181 ttttttaaaa acagactttt aactgtaaag aatctatgta taaacttagc tatgttgtcc
     3241 gtgtatctac aaagaatctt gacgagctaa aaaggcgttg tgatgaagtc aaagactttt
     3301 acgacgactt atcaatcaag ctcgtgcgtc cctttgggga tatgttggga ttgcatggcg
     3361 aatttattcc tgtcagcaaa cgttacatca atgattatat tcaatatgtc acttcggatt
     3421 ttttggctgg acttggtttt ggagcgacac aaacccttgg cgaacatgag gatatctatc
     3481 ttggctacaa tctggacact ggaaagaatg tctatctaaa acctgccctt gctagtcaag
     3541 gggtaaaggg ttctattacc aatgcgttag cgtctgcgtt tatcggttca ttgggtggtg
     3601 gtaaattatt cagtaacaat atgcttgttt actatgcggt actattcggc ggacaggcgg
     3661 tcatagttga ccctaaagca gaacgcggca aatggaaaga aacgctacca gaaatagccc
     3721 atgagataaa cattgtcaat ttaaccagta atgatgaaaa caaaggattg cttgaccctt
     3781 acgtcatttt gagccgtaaa aaggattctg aaagtttagc gattgatatc ctaacattcc
     3841 ttactggtat ctccagtcgt gacagtgata agtttttgca gaaaaatgac aactggaaaa
     3901 taataatgga atagtttcta tgtaaatata tttttactat cttacctaac taattgaaag
     3961 atgatataat ttaatcaaag atttggtaag gagggatgaa tatgcattta attgatttta
     4021 tgaatgaaga tattaaaaag aagcttgtta aaaaaacgta taaaaagaaa gagactattc
     4081 tttttgctga gcaagaaaat gaatatgtaa ttcttatgat tgacggtatt gcagaagcat
     4141 tcatcttaaa tgtgaaagga aacatctcaa cgattcatct ttatagaagt ggtagttttt
     4201 ttggagaaat ggaacaattt aatgatggga aaaaacccgt tgaaattgtc gctttcacag
     4261 attgtaatgt atataaactt catagagatg attttttaaa ctggttaagg ggagattttg
     4321 aagctacaaa atttctgatt cgagaaatat ctagtaaatt agttgtaaat gctgaattaa
     4381 ttgaagaatt atcatcttta agtgttaaag aacgacttct gaggtgtata tccttacact
     4441 attatagaag tgaattaaat ctgcttacca aaaaacagct tgctagcgaa gtaaacgctc
     4501 caattagaag cattaatcgt gctataaatg aatgtgtaca agaacatcta attgcttatg
     4561 aaaacaaaag atttagtgta attaatcagc gagaggtatt aaagaatctt cctacaacat
     4621 aagtataatt tcaaaaaagt attgaataat aattattcaa tgctttttta tttcgggtca
     4681 agtgacctat ttttttttat ttcttcatta tataatactc ctatgcataa ttatgttgga
     4741 ttgatagcta ataaaagaca gtgagaggtg ttatattatg actaaaacac aacagaaaat
     4801 gatacaaaac gtattagaat tttcaaaaag agatagtagg ataataggac ttgcaattag
     4861 cagttcttat atcacaaaat cgttagatga atattctgat ttggattttc tgattgtagc
     4921 agatgataaa tcctatgaag aagttatcga agagagatta cttattgtgg aacagtttgg
     4981 aacattagtt tctgctttta ctggcgaaca cgttggagaa cctcgattga taatttccct
     5041 ctatgattcc cctgttcttc atgttgattt taagtttaca accttagatg gtttactaga
     5101 tagggttgaa gattcagcta ttctctatga agaaaacaac tgcataacag aaatttattc
     5161 taaaaaagaa gcatacttcc caactccaga cttacagtgg attgaagata gattttggat
     5221 ttgggttcat tacgcttgca ctaagattgg acgtagagaa ctatttgaaa caattgattt
     5281 tttatctttt ttaagacaat cagtacttgc cccattaata cagcttctct taggtaaatt
     5341 acctcgtgga gttagaaaaa ttgaattgga cgcaccccaa tatattgaag cattagaaaa
     5401 gacggttgct aaccatgaat ataaaagctg tgttaatgca ttgcaggcta ctatcaacct
     5461 gtatcttgag ttaagagagg aattatcaca aagtcctatt gtccgaaagg ttgaagctga
     5521 aagggttgca ttacagtatt ttcaagattt aatcaaataa acccaaagta aaaaaatatt
     5581 gaatagtaaa tattcagtgt tttttcattt ttggtcaaat gacctagatt agttgttgta
     5641 acaattctat aattattttg aaagtaacag tatggaggat aactatgagt gatattttaa
     5701 gtagaattac aaataaaatt ggggataaga atatagttga taagttatca gctctatcaa
     5761 aatcagattt gaactcactt ctattagaag tgtttgatag acaagcaaat actttaactg
     5821 caactgatat tctaaagtca taccaactaa atagatttac tatccctagc ggtatagatc
     5881 cagaagaaat tcatgctttg gaatcaaaat tattgagaaa agcattcaat atggatatca
     5941 agacgattat gctttctccc tctgccccat taggtagctg tagcgttttt ggctccgtag
     6001 atcaatataa cgtagtaagt gctctacgag ggacagaaac acttgcagac ccctcaaata
     6061 tgttggcaat aattattgct gataaattaa aaagaaaaga agaaaataat accataccta
     6121 tccacatggc tactactgct agggtaataa gagcacaaaa ttttgtaggt aaaggtttat
     6181 attcacattt tggcttatat tgtatggttt catctggtat agatacaggg tcttacactt
     6241 gtgaaaaaat gttgttagaa aagcacttaa actattataa agatatatta agtgaaatag
     6301 aaaatgttcg tttctctatt attctacgca aaagaaacgg atacaaagat aatgacggat
     6361 ttttcgacag aatggttgaa actatcaaac ttatttttcc taatatagag ttatcaattc
     6421 ataatgatga tgttgataat aattattata aaggtatcaa tttcaaatta tcggtcaaac
     6481 aaggaaatga aaccgttgaa atagttgacg gtggatttgt tgactggact tatcaaatgc
     6541 ttggaaataa aagagaacgc tgtttgataa gtggtattgg cttagaaaga tttcttgtag
     6601 ctgtttcata aaaggagaag aaaaatggaa cttatagagt ggaattatgc ttatattacc
     6661 gatttagttg agtttgcgaa tgataaagaa attgcaaaga atcttcgtga caacttcccc
     6721 cacccttata gggagcaaaa cgctagagat tttgttagtt tttgtttggc aacttcagat
     6781 gttaaacaac ttagctatgc tattttttat caagaaaggg ctattggaag tatcagccta
     6841 acatttgaag aagatacctc tattaataaa aatgctgaat taggttactg gcttgctagg
     6901 aactattgag gcaaagggat agcaacaaat gcagtaaaag aaatatgcaa aattgccttt
     6961 gaaaaataca accttaacaa aattcatgct aatgtatatt cctacaatag agagtcaagt
     7021 aaagtattgg aaaaatgtgg ctttaagatt gaagaatact gtcacaattc ataataaaga
     7081 atagaaacat gattgattgc catgtatata gtttaattcg accgcaatag catttattag
     7141 aattaaccta ctttttaata acagaataag tttttcccct ctattaattt cttatgcatc
     7201 tttgtatttt tatgaaaagc tagccaatgc atatttccat acatcaatat ggtggtggct
     7261 tacaaccaat ataaattaaa tacgttttaa agaatacaca tttttgccct ataggcagag
     7321 tgtgtatttt tttctctttt ttttgaaatt tatcgaagat ttaacgccct cccaccgtga
     7381 taggagtaag caaggttatt taactccttg cgatggaaag agggtgaaat cttatggttg
     7441 atgaaaagac aattgaacat caattcgaca gtttctgtaa aacagttttg cgtaactatg
     7501 ctagagatat ttatgacgaa aataaacgca gaaatgatta tttggtatct ctcgaatctt
     7561 tgtcacttgc agaattaagt aaattgagta ttttagatga ttatgattct aactacatct
     7621 gtatggtttc ttacgactac aacatacgaa ttgaagatgt attaatggcg caagcaatcg
     7681 gaaaacttac aaaaagaaaa caagatataa ttctactttc attctttttc aacatgacaa
     7741 atgctgacat agcaacattg atggatttag cagaaaatac catacattat cacaaaacaa
     7801 atgcactaaa agagttgaaa gaactcatgc aggaacattg aaatgaaacc tactaaagaa
     7861 gtattacctt ttacaattat tttacttgct gtacatggcg atgtaaatgc aataaacgta
     7921 atacttaaac attttgaaca ctatatcatt cgtctttcac agaaaacttt atttgatgaa
     7981 tacggaaatc cctatattca tgtagaagaa gaaacaaagc gaatgctagg aacaaaacta
     8041 atcactgcta ttttgagatt taatttgtct taatctcgcc ctctctccat gtatcaagct
     8101 atttggtaca tggagaatca ctaattattt gtctataaaa gcccacaagc tattgtgatt
     8161 ttttataggc aaatcaatgc cgactgttct ttgacaactg aataaagtag atagatacgt
     8221 ttgtgataca gatagccaac aaatactata cgccatgacc ttaatcaaga gagctactat
     8281 atagtgattt gtgaataacc tttggaacgg ttgttttgcc gtaacctgta ccacagtata
     8341 atgatacacc tgtatgacac ggttctgtcc ataagaatgg gattggttga aataccaatg
     8401 gagcttatca aagccatcta tctacttctg aactacgaac atagaaagga gtgatacatt
     8461 atatgaacaa tactgatgta cctatttggg aaaaatatac cttgacgatt gaagaagcgt
     8521 caaaatactt tcgtattgga gagaaaaaat tacgtaaacg tgctgatgaa aaccttgatt
     8581 ctggctgggt cattatgaat agcaatcgca ttcaaataaa gagaaaacaa tttgagaaaa
     8641 tcgttgatac acttgacgtt atctaatgaa attgagcctt atatgttata atattcatat
     8701 gacgtatcaa gctcaattcg gttattgaga ggagcttctt atcatgtcaa ctgaaaaaag
     8761 acgagataat aaagatagaa ttttaagaac tggagagagc cagagaaaag atggaagata
     8821 cgcatataag tatgtatatt catttggtaa accacaattt gtttactctt ggaaacttgt
     8881 acctactgat aaacccccaa agggaaaacg tgatgatata tcattaaggg ataaggaaaa
     8941 agcaatacaa aatgaccttg atgatagcat tgacactatt ggtaagaaaa tgacagtttg
     9001 tcagttatac gctaaaaaga atggactaag aaagaatatc cgacttaaca ctaaaaaagg
     9061 acgccattat cttatgaaga ttcttaacaa ggagtgattc tgtgaagaga tgtattgtag
     9121 tagtggaata gtcaaaaaca taatatatat taaaggagag atttaaatat gactattcca
     9181 agttcaaata aggtatatcc aagaagtaat gattatcaaa ctgtatatct taaaaatgta
     9241 ataacaaggg aaaatataga agttggagat tacacaatat ataatgactt ttataatgac
     9301 ccaagagatt ttgagaaaaa caatgtattg tatcattacc ctataaacaa tgataaatta
     9361 ataattggta agttttgctc aatagcatgt aatgcaaaat ttctaatgac tagtggaaat
     9421 cactcaatga aatcaaaatc cacatatact tttcccatct tttatgaaga atggaaactc
     9481 gatatcaacc atataacgga cgcttgggac aataagggta acattataat tggaaatgat
     9541 gtgtggatag gttatgattc tataattatg tcaggtgtaa atattggtga tggtgcaatt
     9601 attggaacaa gagcagtagt tactaatgac gttccaccgt attctattgt tggaggagta
     9661 cctgcaaaaa ttataaaaaa gagatttaat aatgatacaa tttcaaaatt attagaaatt
     9721 aaatggtggg attggccata caaaaaaatt caatcaaaca ttaagcatat tcaatcagga
     9781 a
//
LOCUS       DAYAOY010000259         9767 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.133, whole genome shotgun sequence.
ACCESSION   DAYAOY010000259 DAYAOY010000000
VERSION     DAYAOY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.133"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..533
                     /locus_tag="ACGI6B_004106"
     CDS             <1..533
                     /locus_tag="ACGI6B_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753460.1"
                     /translation="YKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENI
                     EDIKFIKDGTKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKI
                     KIEREVNIFEDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNV
                     QYTKNRVIILYYIYNH"
     gene            complement(861..1628)
                     /gene="aroD"
                     /locus_tag="ACGI6B_004107"
     CDS             complement(861..1628)
                     /gene="aroD"
                     /locus_tag="ACGI6B_004107"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9753461.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2300..3136)
                     /locus_tag="ACGI6B_004108"
                     /pseudo
     CDS             complement(2300..3136)
                     /locus_tag="ACGI6B_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3207..3875
                     /locus_tag="ACGI6B_004109"
                     /pseudo
     CDS             3207..3875
                     /locus_tag="ACGI6B_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4002..4622
                     /locus_tag="ACGI6B_004110"
     CDS             4002..4622
                     /locus_tag="ACGI6B_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9753462.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4778..5560
                     /locus_tag="ACGI6B_004111"
     CDS             4778..5560
                     /locus_tag="ACGI6B_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9753463.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            5685..6611
                     /locus_tag="ACGI6B_004112"
     CDS             5685..6611
                     /locus_tag="ACGI6B_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753464.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            6625..7074
                     /locus_tag="ACGI6B_004113"
                     /pseudo
     CDS             6625..7074
                     /locus_tag="ACGI6B_004113"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            7488..7841
                     /locus_tag="ACGI6B_004114"
     CDS             7488..7841
                     /locus_tag="ACGI6B_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9753465.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            7843..8073
                     /locus_tag="ACGI6B_004115"
     CDS             7843..8073
                     /locus_tag="ACGI6B_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9753466.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            8463..8666
                     /locus_tag="ACGI6B_004116"
     CDS             8463..8666
                     /locus_tag="ACGI6B_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9753467.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            8744..9076
                     /locus_tag="ACGI6B_004117"
                     /pseudo
     CDS             8744..9076
                     /locus_tag="ACGI6B_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            9169..>9767
                     /locus_tag="ACGI6B_004118"
     CDS             9169..>9767
                     /locus_tag="ACGI6B_004118"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9753468.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWRDWPYKKIQSNIK"
BASE COUNT         3519 a         1448 c         1558 g         3242 t
ORIGIN      
        1 aatataaaaa tggctacatc cctaataaat taaacctttc ttttggatat ggattctctt
       61 cttataaaaa gcaaataatt agagaaactg tagatacttt aagattgaca gaaatttttt
      121 caagcgagaa catagaagat attaaattta taaaagatgg tacaaaaaaa ttagaaatta
      181 gcatagagaa agttgtgaaa tttaaacgtc gaaaaaaaag aaattatgtt tgttgctatt
      241 gccctgatat gtatagagac ataaaactcg acaaagaatc tatcaataag atatacaaca
      301 gaaaaataaa aatagaaaga gaagttaata tttttgaaga tgaggatgtt ataataaaca
      361 agaaagtatt gaagtttcca aagtcttgga caaagaatat gcaaaaatat tggttaagtg
      421 aaaataagta tcccatacat tctactgtaa ttgatgatga tagatataaa tgttgtaatg
      481 tacaatatac aaaaaataga gtgataatat tatattacat atataaccat taatatacat
      541 aattacaaaa aaataatgac aaatatacat aatacaaata tatcttctac tttagataaa
      601 acagttatta tgaaaactta ctatctataa ataatagaaa tgctagtata ccatctataa
      661 tctaatgtct agtaggtttt aatggcatat tttaaatatg ccatagttga tttataagat
      721 tttctatatc actttcaaaa tacacactaa atagttgctt ttcagatata attgcccaat
      781 ttaaatttta ctatctgaaa agttttttat atttaattta tatcttcaaa aataccgtct
      841 aatatttcta tataattaat ttaattaata ctcttatgta atagattaag tactgaattt
      901 aactctttaa atgatatttg ccctggtgct gaaacacttt ttgcagctcc aaaagtcaat
      961 gcagaaccaa atatttcacc acacaatcta cttataacac ccattcctga cattgacatt
     1021 gttattattg gtctatctgc ataaatttta aacatttcat ttgtagcttc aagtagcact
     1081 aacacatctt tttcattttg cggcataaca gctattttgg gtaaatctgc gcccaattcc
     1141 tgcattctac ataaacgaga tacaatctct tctttttttg gagttttatt aaaatcatga
     1201 ttagatataa ttactttcac ttctttttta tgagcaaaat taactacttc atctatgacc
     1261 tcatctccca taaataactc tacatctatt aaatctacta aacctgtatt agaaatttct
     1321 ttattcaaag tagtataata atctcttgat attaattttt ctcccccttc tactacactt
     1381 ctaaatgtga ataataaagg gatatcatgt atataacttc ttaattcata gagaacttcc
     1441 ttaacttctt taatattttc tacattctca aaaaaatcaa ctcgccactc tataatatcc
     1501 aaacatgcat cttttaactc tttagcctct tttataatat cttttttatt tttcccaata
     1561 attggtacac aaattttagg tctaccttct ccaatagtaa tattttttac ttgaactttt
     1621 cttttcatat tctcctccat aatcagaaat tctataggac tttaaattct attttctgca
     1681 caatttaatt atacattaaa aatactatac attataaaaa tactaattta aaaataattt
     1741 ttattctaat tcttaaaaca atcccacata ttttagaaaa ggtactctat aatatacaaa
     1801 cacttctttt ttacctaaat ttattttaac atcctataat tttcacaata ttctttaaat
     1861 tttactcata taaacaatat tattctggat gctaatagaa ttaatctata aatcatagta
     1921 taattatgca gaaataactc gaatttcact acttatgaag agattgaata ttaatgaatc
     1981 taaatcattt gcattactta agagttttga ctaaagttga acaccataca aaagtagcta
     2041 ctcaattgtt tataccccaa ccaagcttaa gtaatgttat atagattctc gaaaaagaat
     2101 ttggtatctt tttatttgaa aaacttatat taaatttgat tatcttttca taagttggta
     2161 aatgtcgata tatcctgtat ctacaaggag tatcggcatt ttctttttgg aagatacttt
     2221 gcataagttg gcatttaacc gtataagaat gccatcaaaa gtagtaaata agtagtaaac
     2281 tctagtcata ctactaggat tataaccgtt ccatttcagc tctcgaagaa ctaaatgaac
     2341 catgtgcata atagccaagc gtcatagtaa tgtttttatg ccccataata tattgcaata
     2401 tattaggact cattcctttg ttagccatat ttgtacagag tgtatgccta aaggtatgtg
     2461 gcgtaatctt tggtagcttg tcctcatggg tcttattata cttcttgact aacgctctta
     2521 acatgctttg ataatgagtt gcttgttttg gtaatccatc ttgtttaaga aataggaagt
     2581 tctggtaacc atcaacaatg attggttctg ctttagcgtt cttacgccgt tccacgatac
     2641 gttcaaatga ttgataagct ctttcagtca ttggaatttc tcgcttaccc tgtgtggtct
     2701 ttggaacttc tacataatag ccaatcttag tatcactcaa caattgatgg tctaccataa
     2761 tggctttatt tttcatatcc aattctaaag tcaaaccgca taattcagaa atgcgtagtc
     2821 ctgtttctaa gaggataaca acatcatcat aatatttaca ataagtctta tcgctcccca
     2881 taaaggataa taagcccatt tcctgttctg gtgttagagc gatttttggt tctgttgtat
     2941 cttccaaaac atcacttaac taaaaattaa atggattctt tctaatacag tcgtcttgaa
     3001 ttgccatgaa aaacgacgct ttcagtgagc gcttataatt gtcaatggat ttataagcac
     3061 acccatcttc actcattcgg atagcccagc ttttagcgtc tgataattta accatatcaa
     3121 tgctttttgc acctagtggg tcattgatag tatctatatt ttgatttata atttatacat
     3181 ttttttaaaa acagactttt aactgtaaag aatctatgta taaacttagc tatgttgtcc
     3241 gtgtatctac aaagaatctt gacgagctaa aaaggcgttg tgatgaagtc aaagactttt
     3301 acgacgactt atcaatcaag ctcgtgcgtc cctttgggga tatgttggga ttgcatggcg
     3361 aatttattcc tgtcagcaaa cgttacatca atgattatat tcaatatgtc acttcggatt
     3421 ttttggctgg acttggtttt ggagcgacac aaacccttgg cgaacatgag gatatctatc
     3481 ttggctacaa tctggacact ggaaagaatg tctatctaaa acctgccctt gctagtcaag
     3541 gggtaaaggg ttctattacc aatgcgttag cgtctgcgtt tatcggttca ttgggtggtg
     3601 gtaaattatt cagtaacaat atgcttgttt actatgcggt actattcggc ggacaggcgg
     3661 tcatagttga ccctaaagca gaacgcggca aatggaaaga aacgctacca gaaatagccc
     3721 atgagataaa cattgtcaat ttaaccagta atgatgaaaa caaaggattg cttgaccctt
     3781 acgtcatttt gagccgtaaa aaggattctg aaagtttagc gattgatatc ctaacattcc
     3841 ttactggtat ctccagtcgt gacagtgata agtttttgca gaaaaatgac aactggaaaa
     3901 taataatgga atagtttcta tgtaaatata tttttactat cttacctaac taattgaaag
     3961 atgatataat ttaatcaaag atttggtaag gagggatgaa tatgcattta attgatttta
     4021 tgaatgaaga tattaaaaag aagcttgtta aaaaaacgta taaaaagaaa gagactattc
     4081 tttttgctga gcaagaaaat gaatatgtaa ttcttatgat tgacggtatt gcagaagcat
     4141 tcatcttaaa tgtgaaagga aacatctcaa cgattcatct ttatagaagt ggtagttttt
     4201 ttggagaaat ggaacaattt aatgatggga aaaaacccgt tgaaattgtc gctttcacag
     4261 attgtaatgt atataaactt catagagatg attttttaaa ctggttaagg ggagattttg
     4321 aagctacaaa atttctgatt cgagaaatat ctagtaaatt agttgtaaat gctgaattaa
     4381 ttgaagaatt atcatcttta agtgttaaag aacgacttct gaggtgtata tccttacact
     4441 attatagaag tgaattaaat ctgcttacca aaaaacagct tgctagcgaa gtaaacgctc
     4501 caattagaag cattaatcgt gctataaatg aatgtgtaca agaacatcta attgcttatg
     4561 aaaacaaaag atttagtgta attaatcagc gagaggtatt aaagaatctt cctacaacat
     4621 aagtataatt tcaaaaaagt attgaataat aattattcaa tgctttttta tttcgggtca
     4681 agtgacctat ttttttttat ttcttcatta tataatactc ctatgcataa ttatgttgga
     4741 ttgatagcta ataaaagaca gtgagaggtg ttatattatg actaaaacac aacagaaaat
     4801 gatacaaaac gtattagaat tttcaaaaag agatagtagg ataataggac ttgcaattag
     4861 cagttcttat atcacaaaat cgttagatga atattctgat ttggattttc tgattgtagc
     4921 agatgataaa tcctatgaag aagttatcga agagagatta cttattgtgg aacagtttgg
     4981 aacattagtt tctgctttta ctggcgaaca cgttggagaa cctcgattga taatttccct
     5041 ctatgattcc cctgttcttc atgttgattt taagtttaca accttagatg gtttactaga
     5101 tagggttgaa gattcagcta ttctctatga agaaaacaac tgcataacag aaatttattc
     5161 taaaaaagaa gcatacttcc caactccaga cttacagtgg attgaagata gattttggat
     5221 ttgggttcat tacgcttgca ctaagattgg acgtagagaa ctatttgaaa caattgattt
     5281 tttatctttt ttaagacaat cagtacttgc cccattaata cagcttctct taggtaaatt
     5341 acctcgtgga gttagaaaaa ttgaattgga cgcaccccaa tatattgaag cattagaaaa
     5401 gacggttgct aaccatgaat ataaaagctg tgttaatgca ttgcaggcta ctatcaacct
     5461 gtatcttgag ttaagagagg aattatcaca aagtcctatt gtccgaaagg ttgaagctga
     5521 aagggttgca ttacagtatt ttcaagattt aatcaaataa acccaaagta aaaaaatatt
     5581 gaatagtaaa tattcagtgt tttttcattt ttggtcaaat gacctagatt agttgttgta
     5641 acaattctat aattattttg aaagtaacag tatggaggat aactatgagt gatattttaa
     5701 gtagaattac aaataaaatt ggggataaga atatagttga taagttatca gctctatcaa
     5761 aatcagattt gaactcactt ctattagaag tgtttgatag acaagcaaat actttaactg
     5821 caactgatat tctaaagtca taccaactaa atagatttac tatccctagc ggtatagatc
     5881 cagaagaaat tcatgctttg gaatcaaaat tattgagaaa agcattcaat atggatatca
     5941 agacgattat gctttctccc tctgccccat taggtagctg tagcgttttt ggctccgtag
     6001 atcaatataa cgtagtaagt gctctacgag ggacagaaac acttgcagac ccctcaaata
     6061 tgttggcaat aattattgct gataaattaa aaagaaaaga agaaaataat accataccta
     6121 tccacatggc tactactgct agggtaataa gagcacaaaa ttttgtaggt aaaggtttat
     6181 attcacattt tggcttatat tgtatggttt catctggtat agatacaggg tcttacactt
     6241 gtgaaaaaat gttgttagaa aagcacttaa actattataa agatatatta agtgaaatag
     6301 aaaatgttcg tttctctatt attctacgca aaagaaacgg atacaaagat aatgacggat
     6361 ttttcgacag aatggttgaa actatcaaac ttatttttcc taatatagag ttatcaattc
     6421 ataatgatga tgttgataat aattattata aaggtatcaa tttcaaatta tcggtcaaac
     6481 aaggaaatga aaccgttgaa atagttgacg gtggatttgt tgactggact tatcaaatgc
     6541 ttggaaataa aagagaacgc tgtttgataa gtggtattgg cttagaaaga tttcttgtag
     6601 ctgtttcata aaaggagaag aaaaatggaa cttatagagt ggaattatgc ttatattacc
     6661 gatttagttg agtttgcgaa tgataaagaa attgcaaaga atcttcgtga caacttcccc
     6721 cacccttata gggagcaaaa cgctagagat tttgttagtt tttgtttggc aacttcagat
     6781 gttaaacaac ttagctatgc tattttttat caagaaaggg ctattggaag tatcagccta
     6841 acatttgaag aagatacctc tattaataaa aatgctgaat taggttactg gcttgctagg
     6901 aactattgag gcaaagggat agcaacaaat gcagtaaaag aaatatgcaa aattgccttt
     6961 gaaaaataca accttaacaa aattcatgct aatgtatatt cctacaatag agagtcaagt
     7021 aaagtattgg aaaaatgtgg ctttaagatt gaagaatact gtcacaattc ataataaaga
     7081 atagaaacat gattgattgc catgtatata gtttaattcg accgcaatag catttattag
     7141 aattaaccta ctttttaata acagaataag tttttcccct ctattaattt cttatgcatc
     7201 tttgtatttt tatgaaaagc tagccaatgc atatttccat acatcaatat ggtggtggct
     7261 tacaaccaat ataaattaaa tacgttttaa agaatacaca tttttgccct ataggcagag
     7321 tgtgtatttt tttctctttt ttttgaaatt tatcgaagat ttaacgccct cccaccgtga
     7381 taggagtaag caaggttatt taactccttg cgatggaaag agggtgaaat cttatggttg
     7441 atgaaaagac aattgaacat caattcgaca gtttctgtaa aacagttttg cgtaactatg
     7501 ctagagatat ttatgacgaa aataaacgca gaaatgatta tttggtatct ctcgaatctt
     7561 tgtcacttgc agaattaagt aaattgagta ttttagatga ttatgattct aactacatct
     7621 gtatggtttc ttacgactac aacatacgaa ttgaagatgt attaatggcg caagcaatcg
     7681 gaaaacttac aaaaagaaaa caagatataa ttctactttc attctttttc aacatgacaa
     7741 atgctgacat agcaacattg atggatttag cagaaaatac catacattat cacaaaacaa
     7801 atgcactaaa agagttgaaa gaactcatgc aggaacattg aaatgaaacc tactaaagaa
     7861 gtattacctt ttacaattat tttacttgct gtacatggcg atgtaaatgc aataaacgta
     7921 atacttaaac attttgaaca ctatatcatt cgtctttcac agaaaacttt atttgatgaa
     7981 tacggaaatc cctatattca tgtagaagaa gaaacaaagc gaatgctagg aacaaaacta
     8041 atcactgcta ttttgagatt taatttgtct taatctcgcc ctctctccat gtatcaagct
     8101 atttggtaca tggagaatca ctaattattt gtctataaaa gcccacaagc tattgtgatt
     8161 ttttataggc aaatcaatgc cgactgttct ttgacaactg aataaagtag atagatacgt
     8221 ttgtgataca gatagccaac aaatactata cgccatgacc ttaatcaaga gagctactat
     8281 atagtgattt gtgaataacc tttggaacgg ttgttttgcc gtaacctgta ccacagtata
     8341 atgatacacc tgtatgacac ggttctgtcc ataagaatgg gattggttga aataccaatg
     8401 gagcttatca aagccatcta tctacttctg aactacgaac atagaaagga gtgatacatt
     8461 atatgaacaa tactgatgta cctatttggg aaaaatatac cttgacgatt gaagaagcgt
     8521 caaaatactt tcgtattgga gagaaaaaat tacgtaaacg tgctgatgaa aaccttgatt
     8581 ctggctgggt cattatgaat agcaatcgca ttcaaataaa gagaaaacaa tttgagaaaa
     8641 tcgttgatac acttgacgtt atctaatgaa attgagcctt atatgttata atattcatat
     8701 gacgtatcaa gctcaattcg gttattgaga ggagcttctt atcatgtcaa ctgaaaaaag
     8761 acgagataat aaagatagaa ttttaagaac tggagagagc cagagaaaag atggaagata
     8821 cgcatataag tatgtatatt catttggtaa accacaattt gtttactctt ggaaacttgt
     8881 acctactgat aaacccccaa agggaaaacg tgatgatata tcattaaggg ataaggaaaa
     8941 agcaatacaa aatgaccttg atgatagcat tgacactatt ggtaagaaaa tgacagtttg
     9001 tcagttatac gctaaaaaga atggactaag aaagaatatc cgacttaaca ctaaaaaagg
     9061 acgccattat cttatgaaga ttcttaacaa ggagtgattc tgtgaagaga tgtattgtag
     9121 tagtggaata gtcaaaaaca taatatatat taaaggagag atttaaatat gactattcca
     9181 agttcaaata aggtatatcc aagaagtaat gattatcaaa ctgtatatct taaaaatgta
     9241 ataacaaggg aaaatataga agttggagat tacacaatat ataatgactt ttataatgac
     9301 ccaagagatt ttgagaaaaa caatgtattg tatcattacc ctataaacaa tgataaatta
     9361 ataattggta agttttgctc aatagcatgt aatgcaaaat ttctaatgac tagtggaaat
     9421 cactcaatga aatcaaaatc cacatatact tttcccatct tttatgaaga atggaaactc
     9481 gatatcaacc atataacgga cgcttgggac aataagggta acattataat tggaaatgat
     9541 gtgtggatag gttatgattc tataattatg tcaggtgtaa atattggtga tggtgcaatt
     9601 attggaacaa gagcagtagt tactaatgac gttccaccgt attctattgt tggaggagta
     9661 cctgcaaaaa ttataaaaaa gagatttaat aatgatacaa tttcaaaatt attagaaatt
     9721 aaatggaggg attggccata taaaaaaatt caatcaaaca ttaagca
//
LOCUS       DAYAOY010000260         9767 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.134, whole genome shotgun sequence.
ACCESSION   DAYAOY010000260 DAYAOY010000000
VERSION     DAYAOY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.134"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..533
                     /locus_tag="ACGI6B_004119"
     CDS             <1..533
                     /locus_tag="ACGI6B_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753469.1"
                     /translation="YKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENI
                     EDIKFIKDGTKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKI
                     KIEREVNIFEDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNV
                     QYTKNRVIILYYIYNH"
     gene            complement(861..1628)
                     /gene="aroD"
                     /locus_tag="ACGI6B_004120"
     CDS             complement(861..1628)
                     /gene="aroD"
                     /locus_tag="ACGI6B_004120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9753470.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2300..3136)
                     /locus_tag="ACGI6B_004121"
                     /pseudo
     CDS             complement(2300..3136)
                     /locus_tag="ACGI6B_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3207..3875
                     /locus_tag="ACGI6B_004122"
                     /pseudo
     CDS             3207..3875
                     /locus_tag="ACGI6B_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4002..4622
                     /locus_tag="ACGI6B_004123"
     CDS             4002..4622
                     /locus_tag="ACGI6B_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9753471.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4778..5560
                     /locus_tag="ACGI6B_004124"
     CDS             4778..5560
                     /locus_tag="ACGI6B_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9753472.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            5685..6611
                     /locus_tag="ACGI6B_004125"
     CDS             5685..6611
                     /locus_tag="ACGI6B_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9753473.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            6625..7074
                     /locus_tag="ACGI6B_004126"
                     /pseudo
     CDS             6625..7074
                     /locus_tag="ACGI6B_004126"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            7488..7841
                     /locus_tag="ACGI6B_004127"
     CDS             7488..7841
                     /locus_tag="ACGI6B_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9753474.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            7843..8073
                     /locus_tag="ACGI6B_004128"
     CDS             7843..8073
                     /locus_tag="ACGI6B_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9753475.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            8463..8666
                     /locus_tag="ACGI6B_004129"
     CDS             8463..8666
                     /locus_tag="ACGI6B_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9753476.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            8744..9076
                     /locus_tag="ACGI6B_004130"
                     /pseudo
     CDS             8744..9076
                     /locus_tag="ACGI6B_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            9169..>9767
                     /locus_tag="ACGI6B_004131"
     CDS             9169..>9767
                     /locus_tag="ACGI6B_004131"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9753477.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIK"
BASE COUNT         3518 a         1448 c         1558 g         3243 t
ORIGIN      
        1 aatataaaaa tggctacatc cctaataaat taaacctttc ttttggatat ggattctctt
       61 cttataaaaa gcaaataatt agagaaactg tagatacttt aagattgaca gaaatttttt
      121 caagcgagaa catagaagat attaaattta taaaagatgg tacaaaaaaa ttagaaatta
      181 gcatagagaa agttgtgaaa tttaaacgtc gaaaaaaaag aaattatgtt tgttgctatt
      241 gccctgatat gtatagagac ataaaactcg acaaagaatc tatcaataag atatacaaca
      301 gaaaaataaa aatagaaaga gaagttaata tttttgaaga tgaggatgtt ataataaaca
      361 agaaagtatt gaagtttcca aagtcttgga caaagaatat gcaaaaatat tggttaagtg
      421 aaaataagta tcccatacat tctactgtaa ttgatgatga tagatataaa tgttgtaatg
      481 tacaatatac aaaaaataga gtgataatat tatattacat atataaccat taatatacat
      541 aattacaaaa aaataatgac aaatatacat aatacaaata tatcttctac tttagataaa
      601 acagttatta tgaaaactta ctatctataa ataatagaaa tgctagtata ccatctataa
      661 tctaatgtct agtaggtttt aatggcatat tttaaatatg ccatagttga tttataagat
      721 tttctatatc actttcaaaa tacacactaa atagttgctt ttcagatata attgcccaat
      781 ttaaatttta ctatctgaaa agttttttat atttaattta tatcttcaaa aataccgtct
      841 aatatttcta tataattaat ttaattaata ctcttatgta atagattaag tactgaattt
      901 aactctttaa atgatatttg ccctggtgct gaaacacttt ttgcagctcc aaaagtcaat
      961 gcagaaccaa atatttcacc acacaatcta cttataacac ccattcctga cattgacatt
     1021 gttattattg gtctatctgc ataaatttta aacatttcat ttgtagcttc aagtagcact
     1081 aacacatctt tttcattttg cggcataaca gctattttgg gtaaatctgc gcccaattcc
     1141 tgcattctac ataaacgaga tacaatctct tctttttttg gagttttatt aaaatcatga
     1201 ttagatataa ttactttcac ttctttttta tgagcaaaat taactacttc atctatgacc
     1261 tcatctccca taaataactc tacatctatt aaatctacta aacctgtatt agaaatttct
     1321 ttattcaaag tagtataata atctcttgat attaattttt ctcccccttc tactacactt
     1381 ctaaatgtga ataataaagg gatatcatgt atataacttc ttaattcata gagaacttcc
     1441 ttaacttctt taatattttc tacattctca aaaaaatcaa ctcgccactc tataatatcc
     1501 aaacatgcat cttttaactc tttagcctct tttataatat cttttttatt tttcccaata
     1561 attggtacac aaattttagg tctaccttct ccaatagtaa tattttttac ttgaactttt
     1621 cttttcatat tctcctccat aatcagaaat tctataggac tttaaattct attttctgca
     1681 caatttaatt atacattaaa aatactatac attataaaaa tactaattta aaaataattt
     1741 ttattctaat tcttaaaaca atcccacata ttttagaaaa ggtactctat aatatacaaa
     1801 cacttctttt ttacctaaat ttattttaac atcctataat tttcacaata ttctttaaat
     1861 tttactcata taaacaatat tattctggat gctaatagaa ttaatctata aatcatagta
     1921 taattatgca gaaataactc gaatttcact acttatgaag agattgaata ttaatgaatc
     1981 taaatcattt gcattactta agagttttga ctaaagttga acaccataca aaagtagcta
     2041 ctcaattgtt tataccccaa ccaagcttaa gtaatgttat atagattctc gaaaaagaat
     2101 ttggtatctt tttatttgaa aaacttatat taaatttgat tatcttttca taagttggta
     2161 aatgtcgata tatcctgtat ctacaaggag tatcggcatt ttctttttgg aagatacttt
     2221 gcataagttg gcatttaacc gtataagaat gccatcaaaa gtagtaaata agtagtaaac
     2281 tctagtcata ctactaggat tataaccgtt ccatttcagc tctcgaagaa ctaaatgaac
     2341 catgtgcata atagccaagc gtcatagtaa tgtttttatg ccccataata tattgcaata
     2401 tattaggact cattcctttg ttagccatat ttgtacagag tgtatgccta aaggtatgtg
     2461 gcgtaatctt tggtagcttg tcctcatggg tcttattata cttcttgact aacgctctta
     2521 acatgctttg ataatgagtt gcttgttttg gtaatccatc ttgtttaaga aataggaagt
     2581 tctggtaacc atcaacaatg attggttctg ctttagcgtt cttacgccgt tccacgatac
     2641 gttcaaatga ttgataagct ctttcagtca ttggaatttc tcgcttaccc tgtgtggtct
     2701 ttggaacttc tacataatag ccaatcttag tatcactcaa caattgatgg tctaccataa
     2761 tggctttatt tttcatatcc aattctaaag tcaaaccgca taattcagaa atgcgtagtc
     2821 ctgtttctaa gaggataaca acatcatcat aatatttaca ataagtctta tcgctcccca
     2881 taaaggataa taagcccatt tcctgttctg gtgttagagc gatttttggt tctgttgtat
     2941 cttccaaaac atcacttaac taaaaattaa atggattctt tctaatacag tcgtcttgaa
     3001 ttgccatgaa aaacgacgct ttcagtgagc gcttataatt gtcaatggat ttataagcac
     3061 acccatcttc actcattcgg atagcccagc ttttagcgtc tgataattta accatatcaa
     3121 tgctttttgc acctagtggg tcattgatag tatctatatt ttgatttata atttatacat
     3181 ttttttaaaa acagactttt aactgtaaag aatctatgta taaacttagc tatgttgtcc
     3241 gtgtatctac aaagaatctt gacgagctaa aaaggcgttg tgatgaagtc aaagactttt
     3301 acgacgactt atcaatcaag ctcgtgcgtc cctttgggga tatgttggga ttgcatggcg
     3361 aatttattcc tgtcagcaaa cgttacatca atgattatat tcaatatgtc acttcggatt
     3421 ttttggctgg acttggtttt ggagcgacac aaacccttgg cgaacatgag gatatctatc
     3481 ttggctacaa tctggacact ggaaagaatg tctatctaaa acctgccctt gctagtcaag
     3541 gggtaaaggg ttctattacc aatgcgttag cgtctgcgtt tatcggttca ttgggtggtg
     3601 gtaaattatt cagtaacaat atgcttgttt actatgcggt actattcggc ggacaggcgg
     3661 tcatagttga ccctaaagca gaacgcggca aatggaaaga aacgctacca gaaatagccc
     3721 atgagataaa cattgtcaat ttaaccagta atgatgaaaa caaaggattg cttgaccctt
     3781 acgtcatttt gagccgtaaa aaggattctg aaagtttagc gattgatatc ctaacattcc
     3841 ttactggtat ctccagtcgt gacagtgata agtttttgca gaaaaatgac aactggaaaa
     3901 taataatgga atagtttcta tgtaaatata tttttactat cttacctaac taattgaaag
     3961 atgatataat ttaatcaaag atttggtaag gagggatgaa tatgcattta attgatttta
     4021 tgaatgaaga tattaaaaag aagcttgtta aaaaaacgta taaaaagaaa gagactattc
     4081 tttttgctga gcaagaaaat gaatatgtaa ttcttatgat tgacggtatt gcagaagcat
     4141 tcatcttaaa tgtgaaagga aacatctcaa cgattcatct ttatagaagt ggtagttttt
     4201 ttggagaaat ggaacaattt aatgatggga aaaaacccgt tgaaattgtc gctttcacag
     4261 attgtaatgt atataaactt catagagatg attttttaaa ctggttaagg ggagattttg
     4321 aagctacaaa atttctgatt cgagaaatat ctagtaaatt agttgtaaat gctgaattaa
     4381 ttgaagaatt atcatcttta agtgttaaag aacgacttct gaggtgtata tccttacact
     4441 attatagaag tgaattaaat ctgcttacca aaaaacagct tgctagcgaa gtaaacgctc
     4501 caattagaag cattaatcgt gctataaatg aatgtgtaca agaacatcta attgcttatg
     4561 aaaacaaaag atttagtgta attaatcagc gagaggtatt aaagaatctt cctacaacat
     4621 aagtataatt tcaaaaaagt attgaataat aattattcaa tgctttttta tttcgggtca
     4681 agtgacctat ttttttttat ttcttcatta tataatactc ctatgcataa ttatgttgga
     4741 ttgatagcta ataaaagaca gtgagaggtg ttatattatg actaaaacac aacagaaaat
     4801 gatacaaaac gtattagaat tttcaaaaag agatagtagg ataataggac ttgcaattag
     4861 cagttcttat atcacaaaat cgttagatga atattctgat ttggattttc tgattgtagc
     4921 agatgataaa tcctatgaag aagttatcga agagagatta cttattgtgg aacagtttgg
     4981 aacattagtt tctgctttta ctggcgaaca cgttggagaa cctcgattga taatttccct
     5041 ctatgattcc cctgttcttc atgttgattt taagtttaca accttagatg gtttactaga
     5101 tagggttgaa gattcagcta ttctctatga agaaaacaac tgcataacag aaatttattc
     5161 taaaaaagaa gcatacttcc caactccaga cttacagtgg attgaagata gattttggat
     5221 ttgggttcat tacgcttgca ctaagattgg acgtagagaa ctatttgaaa caattgattt
     5281 tttatctttt ttaagacaat cagtacttgc cccattaata cagcttctct taggtaaatt
     5341 acctcgtgga gttagaaaaa ttgaattgga cgcaccccaa tatattgaag cattagaaaa
     5401 gacggttgct aaccatgaat ataaaagctg tgttaatgca ttgcaggcta ctatcaacct
     5461 gtatcttgag ttaagagagg aattatcaca aagtcctatt gtccgaaagg ttgaagctga
     5521 aagggttgca ttacagtatt ttcaagattt aatcaaataa acccaaagta aaaaaatatt
     5581 gaatagtaaa tattcagtgt tttttcattt ttggtcaaat gacctagatt agttgttgta
     5641 acaattctat aattattttg aaagtaacag tatggaggat aactatgagt gatattttaa
     5701 gtagaattac aaataaaatt ggggataaga atatagttga taagttatca gctctatcaa
     5761 aatcagattt gaactcactt ctattagaag tgtttgatag acaagcaaat actttaactg
     5821 caactgatat tctaaagtca taccaactaa atagatttac tatccctagc ggtatagatc
     5881 cagaagaaat tcatgctttg gaatcaaaat tattgagaaa agcattcaat atggatatca
     5941 agacgattat gctttctccc tctgccccat taggtagctg tagcgttttt ggctccgtag
     6001 atcaatataa cgtagtaagt gctctacgag ggacagaaac acttgcagac ccctcaaata
     6061 tgttggcaat aattattgct gataaattaa aaagaaaaga agaaaataat accataccta
     6121 tccacatggc tactactgct agggtaataa gagcacaaaa ttttgtaggt aaaggtttat
     6181 attcacattt tggcttatat tgtatggttt catctggtat agatacaggg tcttacactt
     6241 gtgaaaaaat gttgttagaa aagcacttaa actattataa agatatatta agtgaaatag
     6301 aaaatgttcg tttctctatt attctacgca aaagaaacgg atacaaagat aatgacggat
     6361 ttttcgacag aatggttgaa actatcaaac ttatttttcc taatatagag ttatcaattc
     6421 ataatgatga tgttgataat aattattata aaggtatcaa tttcaaatta tcggtcaaac
     6481 aaggaaatga aaccgttgaa atagttgacg gtggatttgt tgactggact tatcaaatgc
     6541 ttggaaataa aagagaacgc tgtttgataa gtggtattgg cttagaaaga tttcttgtag
     6601 ctgtttcata aaaggagaag aaaaatggaa cttatagagt ggaattatgc ttatattacc
     6661 gatttagttg agtttgcgaa tgataaagaa attgcaaaga atcttcgtga caacttcccc
     6721 cacccttata gggagcaaaa cgctagagat tttgttagtt tttgtttggc aacttcagat
     6781 gttaaacaac ttagctatgc tattttttat caagaaaggg ctattggaag tatcagccta
     6841 acatttgaag aagatacctc tattaataaa aatgctgaat taggttactg gcttgctagg
     6901 aactattgag gcaaagggat agcaacaaat gcagtaaaag aaatatgcaa aattgccttt
     6961 gaaaaataca accttaacaa aattcatgct aatgtatatt cctacaatag agagtcaagt
     7021 aaagtattgg aaaaatgtgg ctttaagatt gaagaatact gtcacaattc ataataaaga
     7081 atagaaacat gattgattgc catgtatata gtttaattcg accgcaatag catttattag
     7141 aattaaccta ctttttaata acagaataag tttttcccct ctattaattt cttatgcatc
     7201 tttgtatttt tatgaaaagc tagccaatgc atatttccat acatcaatat ggtggtggct
     7261 tacaaccaat ataaattaaa tacgttttaa agaatacaca tttttgccct ataggcagag
     7321 tgtgtatttt tttctctttt ttttgaaatt tatcgaagat ttaacgccct cccaccgtga
     7381 taggagtaag caaggttatt taactccttg cgatggaaag agggtgaaat cttatggttg
     7441 atgaaaagac aattgaacat caattcgaca gtttctgtaa aacagttttg cgtaactatg
     7501 ctagagatat ttatgacgaa aataaacgca gaaatgatta tttggtatct ctcgaatctt
     7561 tgtcacttgc agaattaagt aaattgagta ttttagatga ttatgattct aactacatct
     7621 gtatggtttc ttacgactac aacatacgaa ttgaagatgt attaatggcg caagcaatcg
     7681 gaaaacttac aaaaagaaaa caagatataa ttctactttc attctttttc aacatgacaa
     7741 atgctgacat agcaacattg atggatttag cagaaaatac catacattat cacaaaacaa
     7801 atgcactaaa agagttgaaa gaactcatgc aggaacattg aaatgaaacc tactaaagaa
     7861 gtattacctt ttacaattat tttacttgct gtacatggcg atgtaaatgc aataaacgta
     7921 atacttaaac attttgaaca ctatatcatt cgtctttcac agaaaacttt atttgatgaa
     7981 tacggaaatc cctatattca tgtagaagaa gaaacaaagc gaatgctagg aacaaaacta
     8041 atcactgcta ttttgagatt taatttgtct taatctcgcc ctctctccat gtatcaagct
     8101 atttggtaca tggagaatca ctaattattt gtctataaaa gcccacaagc tattgtgatt
     8161 ttttataggc aaatcaatgc cgactgttct ttgacaactg aataaagtag atagatacgt
     8221 ttgtgataca gatagccaac aaatactata cgccatgacc ttaatcaaga gagctactat
     8281 atagtgattt gtgaataacc tttggaacgg ttgttttgcc gtaacctgta ccacagtata
     8341 atgatacacc tgtatgacac ggttctgtcc ataagaatgg gattggttga aataccaatg
     8401 gagcttatca aagccatcta tctacttctg aactacgaac atagaaagga gtgatacatt
     8461 atatgaacaa tactgatgta cctatttggg aaaaatatac cttgacgatt gaagaagcgt
     8521 caaaatactt tcgtattgga gagaaaaaat tacgtaaacg tgctgatgaa aaccttgatt
     8581 ctggctgggt cattatgaat agcaatcgca ttcaaataaa gagaaaacaa tttgagaaaa
     8641 tcgttgatac acttgacgtt atctaatgaa attgagcctt atatgttata atattcatat
     8701 gacgtatcaa gctcaattcg gttattgaga ggagcttctt atcatgtcaa ctgaaaaaag
     8761 acgagataat aaagatagaa ttttaagaac tggagagagc cagagaaaag atggaagata
     8821 cgcatataag tatgtatatt catttggtaa accacaattt gtttactctt ggaaacttgt
     8881 acctactgat aaacccccaa agggaaaacg tgatgatata tcattaaggg ataaggaaaa
     8941 agcaatacaa aatgaccttg atgatagcat tgacactatt ggtaagaaaa tgacagtttg
     9001 tcagttatac gctaaaaaga atggactaag aaagaatatc cgacttaaca ctaaaaaagg
     9061 acgccattat cttatgaaga ttcttaacaa ggagtgattc tgtgaagaga tgtattgtag
     9121 tagtggaata gtcaaaaaca taatatatat taaaggagag atttaaatat gactattcca
     9181 agttcaaata aggtatatcc aagaagtaat gattatcaaa ctgtatatct taaaaatgta
     9241 ataacaaggg aaaatataga agttggagat tacacaatat ataatgactt ttataatgac
     9301 ccaagagatt ttgagaaaaa caatgtattg tatcattacc ctataaacaa tgataaatta
     9361 ataattggta agttttgctc aatagcatgt aatgcaaaat ttctaatgac tagtggaaat
     9421 cactcaatga aatcaaaatc cacatatact tttcccatct tttatgaaga atggaaactc
     9481 gatatcaacc atataacgga cgcttgggac aataagggta acattataat tggaaatgat
     9541 gtgtggatag gttatgattc tataattatg tcaggtgtaa atattggtga tggtgcaatt
     9601 attggaacaa gagcagtagt tactaatgac gttccaccgt attctattgt tggaggagta
     9661 cctgcaaaaa ttataaaaaa gagatttaat aatgatacaa tttcaaaatt attagaaatt
     9721 aaatggtggg attggccata taaaaaaatt caatcaaaca ttaagca
//
LOCUS       DAYAOY010000261         7976 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.140, whole genome shotgun sequence.
ACCESSION   DAYAOY010000261 DAYAOY010000000
VERSION     DAYAOY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.140"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            complement(<1..1059)
                     /locus_tag="ACGI6B_004132"
     CDS             complement(<1..1059)
                     /locus_tag="ACGI6B_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFG9753478.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILI"
     gene            complement(1073..2002)
                     /locus_tag="ACGI6B_004133"
     CDS             complement(1073..2002)
                     /locus_tag="ACGI6B_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9753479.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            2223..2408
                     /locus_tag="ACGI6B_004134"
     CDS             2223..2408
                     /locus_tag="ACGI6B_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9753480.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            2401..3102
                     /locus_tag="ACGI6B_004135"
     CDS             2401..3102
                     /locus_tag="ACGI6B_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9753481.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3104..4027
                     /locus_tag="ACGI6B_004136"
     CDS             3104..4027
                     /locus_tag="ACGI6B_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9753482.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4184..5107
                     /locus_tag="ACGI6B_004137"
     CDS             4184..5107
                     /locus_tag="ACGI6B_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753483.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5100..5837
                     /locus_tag="ACGI6B_004138"
     CDS             5100..5837
                     /locus_tag="ACGI6B_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753484.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            5848..6621
                     /locus_tag="ACGI6B_004139"
     CDS             5848..6621
                     /locus_tag="ACGI6B_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753485.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7162..7770)
                     /locus_tag="ACGI6B_004140"
     CDS             complement(7162..7770)
                     /locus_tag="ACGI6B_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFG9753486.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2661 a         1297 c         1206 g         2812 t
ORIGIN      
        1 tattaaaatt cttgacagac tttcttttaa tctctttatt tgctgtctat acattctagc
       61 atgttctcta aattttccta aattattttc tttttgtctt gttacatatt ttagttgaaa
      121 tacattatta cactcacttc taagaactcc atctgtaaag tgtattctac aaccttcatg
      181 aacatttcta caattttgtg tctcaatttt tttaatttca cctttactca aataagtttt
      241 tccataataa tgttctattt ttgaatatat cttatttgca gtgtcttcat caacatatat
      301 aactcggtct gctgaattgc tacatttaga atcatcaaaa tcccctacac cttctacaag
      361 catggatgaa gatattgaat ctatatcact ttcaataata ttttcttctt catcactata
      421 aagttcttca tacatgaaat cagcaaatgt atcaaaattt acttctacac tttctacatt
      481 attttcatca ataagactat tttcttctcc atttaatatg tattcaaaat aagttaaata
      541 tataacatct aatgctttta aaatctccat aatatcttga ttggtatcta tatttcttat
      601 atcattaact attcttctag tcatactatc taatgctggg tgcttgtcca tactataaag
      661 tacaaatgtg tatttaattt tatccataat attatcattg tgtattttgg taaaacgtga
      721 aacaatatct ttataagcat tccttcttat gtcttctatc cctggcctgt ctttaatgac
      781 cttttcttca actattgtat ttaatataac ttgaattaga cctagtataa catctttgtt
      841 gtatcctttt cttattctat ttgtaagata ttttttaact atatcccaat ctacatactt
      901 tcttctagca cctgcaatta ttgcgaaata tagacctatt tcttttgaag agtaatcttt
      961 ttctgatata tctatacttt cattgtaatc accagctatt gtccatgata ggttattagc
     1021 tctattttca aattcataat catcatatat ccatttcata atctcaccta tattaaatat
     1081 gacttattgg aaacacatct atactctccc atttattagg tattctagtt ttaattacat
     1141 ctccaaccat ttctttctca tatacatcaa aagtctttcc tgtaagtccc atattaattg
     1201 caagagttgg ttttaatcct cttcttatag tcttaagaga tgccataaga ccacgtaaat
     1261 caatagcttt actagatatc tctccatttt gagacttcaa ttgtaaatct aaaaatattc
     1321 ctgcaaagtg tattaatttt tcttcatcag catctgaaaa ttcatttttt aaaatcatca
     1381 ttaacttatc ttcttctata ggaggaatat ctattaccat aaatctagat accagtgctt
     1441 cattaagttc ttttgttcct gcatattcat aattcatagt cccaataaat cttgttgctg
     1501 gatgtagatt gactctctca tacccaggta catcaattat tcttctatag tctaaagctg
     1561 aatgtaccac aactatagca tcattttttg ccatattaat ttcatcaaac accccaaaac
     1621 ctccattaat agcacactca taaactgagc ctcttctaag tttaacttca ttgtctataa
     1681 atgtatctgt acctataagt gttgaactgt ctgtatttat atgaaatgat gtattccact
     1741 gaggtctatt aaatatttcc cctagattat cagcaagtaa gttttttcct gttgcctttg
     1801 gtcccgataa taatatattt tcttcttcta aaatagctgc tatacacata gacaagatat
     1861 ctttaccata aaaataagtt ttagactttg ttactctgta ttctaaatct ttttctaaac
     1921 cataataatt tctaaaatac agtacatctt caataagttt ttcatctacc ccttgcaccc
     1981 ttagattatc tattatatcc atatttttac cccttttata taaatagtat ttgtttcatt
     2041 tcttatcacc attttagcat aaaaggatat aagctaaaat tgatttttta atctccccat
     2101 tttaagtaaa accatgcacc attttatcac tacaaaatta agagtactac ttactgatat
     2161 ttcttgattt tgttcgtcta tacgtatata atatatgctg ataaattatg aggaaaggat
     2221 gattgaaata tggatgccca gaagtagcaa aaaaatgggg tatttctgaa agaagagtac
     2281 aaaaactatg tgaagaaagt cgcataccag gagtttcaaa actcgggtat atgtggctga
     2341 taccaaagaa cgcagaaaag cctgttgatg gtagaacaaa aagaagaaag gaagtacaat
     2401 atgaatagcg ttttgattat agatgatgac aaagaacttt gtgctcttat gaaaaaatgt
     2461 gttgagcaag aaaatttatc tgctattact gcacattgtg gtgcagaagg tctacatttg
     2521 gtcgataaaa atagacacac actctctctt gtaattttgg acataatgat gccagatata
     2581 gacggttttc aagttctgaa aaaaattaga gaaacaagta atattccagt aattatgctt
     2641 actgctaaaa atgacgagga tgataaagtt tccggactac gtttaggtgc tgacgattac
     2701 ctaacaaaac cgttcagcat aaacgaactt atggctcgtg tcaattctct tattcgtcgg
     2761 tatactatgt taaaccctgc ttttataaca gacacagatt gcataataat aaatggcatg
     2821 attattgaca agacaaaccg aattgtttct gtcaataata ttcctgttca acttacaagc
     2881 aaagaatttg atttacttgc ttttctggct tctaataagg gacgggtgtt taccaaaaaa
     2941 cagatttata cacaagtatg ggaagaagaa tatgcttttg acgatagtaa catcatgtct
     3001 ttcatcagta aattgagaaa aaagattgaa ccagacccca accatccatt ttatattttg
     3061 acagttcgtg gtgtcggtta tcgttttaat aaggaggctt aacatgaata caagtgtcta
     3121 tctgcttttg atattggttc ttagcctgat tattatagcg taccttgttt ctgtcctgct
     3181 acgtgtccga atacaactta cacttataaa agatgcttta gaggatataa aaaatggaaa
     3241 ccttaatcga cgtattttaa cagatgaaaa cgacattaca aaacaaattt gctatgatat
     3301 taacgaaata gcaatcaata gccaatttca acttattcag caaaaacaat cagaacaagc
     3361 ctacaaacag ctaatgacaa gtctctccca tgatgtaaaa actccacttg cttctttggt
     3421 cggttatttg gaagcagtag aaagtaaaat tgttgtagga gaggaaaaag acgagtatat
     3481 ccatgttgcg tctaataagg ctcattactt aaagaatttt gtagaaaatc tatttgaatg
     3541 ggtaaaattg gattctaagg aacaagtttt tcattttgat atttttgact tgaatgaatt
     3601 atcccgtaat attatatctg attggatacc tgttttagaa agcagtcatt ttgaatatga
     3661 atttgatata ccagaaatag agtacttctt acgtatagac gcaaatgcat atacccgtat
     3721 cattaataac ctattacaaa atattattac ccatagtagt ggcaacaaaa tgacattgag
     3781 aatttttgaa aacaaagagc aagctcaaat tataataaca gataatggga aaggtatatc
     3841 ttcagacaat ctcccacata tttttgaaag gttgtaccaa tgcgaccatt cacgagcttc
     3901 aaagggaaat ggtttaggct tggcgattgc aaaggaactt attaacgctc ataagggaaa
     3961 tattaccgcc aacagcactc ccggtatggg aacagaattt acaatattgc ttcctaaagc
     4021 cctgtaaaag tacgaggctt tcttactcta aaacaagata aaagcaagat tatggcaagg
     4081 ttttggcaag gttcatgttt tataattcta attgtagttg attttgacgt tgtatcaact
     4141 atcttttgta gaaacaaatt tattaaaagt gaggaaatta tatatgaatg attttgtgat
     4201 tgaaacaaag caacttacaa aaatttatgg gaatcaaaca gtagttgaca aagtcaatct
     4261 ccatgcaaaa aaaggccgaa tatatggtct gttaggacgc aacggagctg ggaaaaccac
     4321 aattatgaaa atgattttag gattgacttc aatcacgtat ggcgaggtag atgtatttgg
     4381 agagaatatt aagggacgtg aaaaacaagt atatccccgt atcggtgcaa ttatcgaaac
     4441 gccaggattt tatcctaatc tgacaggaac ggagaacttg gagatttttg caaaactgcg
     4501 tggtacagcc gcccctaatt ctgtaaaaag agcattggaa attgtaggct taccttataa
     4561 aaataaaaag ctgtttagtg aatattcact tggcatgaaa cagcgacttg gtattgccaa
     4621 cgctatcctg catgacccag aactgttagt tttagatgag ccaaccaatg gtcttgaccc
     4681 tattggtatt gcggagatga gaaagtttat tagggacttg agcgttgagc atggaaagac
     4741 aattcttatt tccagtcata ttctttcaga aatctcattg ttggctgatg atattggaat
     4801 tatcgaccac ggtgttcttt tggaagaaag tagtatgagt gaactagaaa agaaaaacag
     4861 taaatatatc ctgctacaag tttcagatat tccgaagaca tcacttattt tagagcgtca
     4921 atttaacacg aaggattatt ccgttcaaga tgactacacg ttacaccttt ataacactaa
     4981 tctggatatg ggtgaaatca ataaagcact tgtaatgcag aatgtaactg ttattagttc
     5041 tcaactttgt aatgatactt tagaggacta ctttaaaaaa atcacaggag gagagggcat
     5101 tgcttaaact tattcaaaca gaatttctta aactgcgtcg taggaaactt atttggctga
     5161 tgttgttgtc tgctctcatt atgccgtttt tcgcactact atacttcaat tattttggta
     5221 aaacaggtgt tgagccaata aagttttata aatggtctgc atttggttat acactttgga
     5281 ttattttgcc tgttgttttg ggtatatttt gcaccatgct tatacacgaa gaaaaccagt
     5341 atgatatgct caaacagctt tggattgtac ctattagtaa aatgggatac ttttttagta
     5401 aatttttcgt tgttttgatt tactctattt gttttatgct gattacagcc gttgcttctg
     5461 tgttatttag tgtactttct ggatatgtcg tatttagctg ggcaagcatt ttatatttgt
     5521 tgaaaaaatg tttagaaatt ggtgtaatca ctgcttttgt aatgttgccg attttggcaa
     5581 tcgctacatc tcaaaaagga tacattctcc caatttgtat cacattggta tatgcctttt
     5641 ccggttttgt tataatgaca ataaatatgt atttacaccc attatccagt atgggagtta
     5701 ttatcatgag aaataaagac attccaggtc ttatattttc acaggaaatt aatgtcccat
     5761 tagcatttct ttgtatttgt atttggagta ttgtatcagt attgtttgca aacactgcac
     5821 taggaagaag aaagtgaggt gtagataatg tggaaattac tttgggctga atgtcaaaaa
     5881 attcgtcgtt caaaaattgt ttggattaca gtttttgcat ccattatgat agcagtcata
     5941 gtttttgcag gagggcagga tgtatataat gacccagatt tacattatgg cttaaagagc
     6001 gtttatgacg gttcacggta tatagacaat gcaggttggt atatggacgc agtacaacct
     6061 tggtcaactt tttttgttct tcctgctgtc attgccctat taggaagcta tattatttgt
     6121 cgtgaagaag aggaggacac tattaaatcc ctgcgtctta ttcccataaa tgaaacaaaa
     6181 ttaaccatag caaaaatgat tattactttt atattcagta tacttcttta tttgttactc
     6241 tttactataa catttttgac tgaatccgtt ttacattttt ccgatttatc gacaaaactg
     6301 gttttaagtt gcatgaaaga gtattttctt gatggcatag gtgtattctt tgcaatttct
     6361 cccattattg ccttggtatc gcgcatgaaa aaaggatatt ggcttgcatt agtattcaca
     6421 gaagtttatt ctattgctgg actatttgcg ggtatgtcaa atgtcttaca aacctttttt
     6481 cctattgcgg caatatttaa tatttctgga tactatataa ctactggaga aaaaattatg
     6541 gggagtatca taagtttatt actttgtgcc tgcctttctg catttatact taaagggcta
     6601 aaacacaatg aaaaaaattg aaataggtat atcaaaatga aaaagataaa aaaactgatt
     6661 attctaattg tattcttgtt tctttttggc atgttacact gttatactga atggaaattc
     6721 aacaataacc cacctaaagg ggtaatagaa agaataaatg aatggactga taagattgga
     6781 ctggattagt aacttatgtt aattatagtg aatgtaatat tagcaagtta ttgtttcgta
     6841 ttttaatact ataactttaa atatttggtt ttaatcttcc aaacgaatat aaaaaaactg
     6901 tcacagaggc aacatatttt tgaatattta taaagtggta gttttgattt tcaaagctgc
     6961 cgccttttct tctatataag caaacttatc ctcaacaaag tgtaaaacat ggtacatttt
     7021 aagaaaaaag taccacagac acaagcaata aaagaaacgc cgagaaccta caaaaacgta
     7081 gtgcttatgc agatttacga cgccttctat tttctcaacc gtcttaatga ccttgtacaa
     7141 aagaaatttt tctaatattt tttataatct tatcttgtga tgttttttat gtataaatga
     7201 cttaataaca aatccaataa aaattatgga tattggtata attagaacga ggaatttata
     7261 atcattataa tgtgacatta ttcttaccca actctcagaa aaaatatatc ctatactgat
     7321 taatgaagta ttccatatgg ttattcccac gatagatgat attacaaaag ttataatatt
     7381 ttgacctgct atacctgcta taaaagatat gtatgttcta cataaaggga ttaatctaca
     7441 tacacaaaca gaaatagaag agtatttgtt aaaataatct tgagatgcaa atattccttt
     7501 ctttgccttt ggacatatct ctattatttt atttatgata gatttacctc cccaatttcc
     7561 cactgtataa caaaatatcg aaccaagtat acctgcaatt atacttaaaa ttaaaattgt
     7621 cagaaatcca aaatgatttc ttgcagcaat tgctccacat aaaggtaaaa caacttcgct
     7681 aggtaatgga aaacaagcat actcaatcat aattaataca aatacagaga ttaatccata
     7741 ttgtaaaata aaactattga ttacagacac ttacttatct atctcctttc ttatttaaag
     7801 gttttaaggg tacatgttct aaggattctc accctatatt taaagaatca atctctttaa
     7861 actattatat gtatttacac aaagcattta taacatactt taaaaatagt accttaaaat
     7921 aagaaactta ataaataaaa aaagattacg tggctacgtc ctactctccc aggcag
//
LOCUS       DAYAOY010000262         5236 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.179, whole genome shotgun sequence.
ACCESSION   DAYAOY010000262 DAYAOY010000000
VERSION     DAYAOY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.179"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            <1..313
                     /locus_tag="ACGI6B_004141"
     CDS             <1..313
                     /locus_tag="ACGI6B_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFG9753487.1"
                     /translation="NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMT
                     PQQAMEIALKEQNGIVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKE
                     D"
     gene            421..2952
                     /locus_tag="ACGI6B_004142"
     CDS             421..2952
                     /locus_tag="ACGI6B_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9753488.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3386..4087
                     /locus_tag="ACGI6B_004143"
     CDS             3386..4087
                     /locus_tag="ACGI6B_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFG9753489.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4137..5219
                     /gene="vanS"
                     /locus_tag="ACGI6B_004144"
     CDS             4137..5219
                     /gene="vanS"
                     /locus_tag="ACGI6B_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFG9753490.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
BASE COUNT         2040 a          528 c          971 g         1697 t
ORIGIN      
        1 taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag aagataaaac
       61 agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat tagatttaaa
      121 tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa atggaatagt
      181 taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa gaatagataa
      241 agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat tgaaggtaga
      301 aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga ataaattttg
      361 tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga ggagtacggt
      421 atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc gcttgaccct
      481 gatttaagag tatttgacat aattatgtat acaccttatg gaactactta taattcatat
      541 gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa gcattttgac
      601 aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga ctatattgtt
      661 gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga tttagctaaa
      721 aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat cgtaaacaaa
      781 gactttgaat atgttgcagt tacagatgga gatacattat caattgggga caaaacttta
      841 gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata tataaaagag
      901 gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga taagatagta
      961 aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata ctattatgat
     1021 tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat aaaagattta
     1081 gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc tagaaaaatt
     1141 atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa agaagttaca
     1201 atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat aaaagcatat
     1261 attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca taagcaagaa
     1321 gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc aacaatatta
     1381 ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt tcttcatggt
     1441 ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat agaaaatgct
     1501 atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt taactttaaa
     1561 ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct agataagtta
     1621 aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat ttgtaatgaa
     1681 gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa ggaagaccaa
     1741 tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga atatttcgta
     1801 atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa aagaaataaa
     1861 acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag acctgcactt
     1921 tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa agattggtat
     1981 gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga tgaagtgaat
     2041 aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat agctacagga
     2101 agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt tactcttaga
     2161 aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa agttgtagtg
     2221 attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa aggattagaa
     2281 gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa agaaggtagt
     2341 aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg tgtagcagtt
     2401 gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg agatagtgtc
     2461 gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat ggttcaggat
     2521 acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac aaatgttaaa
     2581 gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc aatatggcca
     2641 tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa tttgacattt
     2701 gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt tagtattggt
     2761 aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag aatatataaa
     2821 aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga tttaagctgt
     2881 actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat tatgaaggct
     2941 gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat tgattttttt
     3001 ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa catggaaatt
     3061 ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga ttgtctctgg
     3121 tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta atacctgtta
     3181 tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc agtttttgtg
     3241 ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa aaattagtta
     3301 caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg atgacctcga
     3361 taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg atgatgaaaa
     3421 agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag tatacaagtt
     3481 ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata tggctatttt
     3541 agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc gagaaaaata
     3601 ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga taatgggttt
     3661 aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag ttgttgcaag
     3721 agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac aacagtcgat
     3781 aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac ataaatgtag
     3841 tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt ggtatttatg
     3901 tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat ggggagaaaa
     3961 atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg aaaaaattca
     4021 tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt atacaattga
     4081 aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa atttttatga
     4141 gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat tcttttatat
     4201 tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt cgtatggaat
     4261 ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag ctcttcatga
     4321 tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat tggtttacaa
     4381 aatattttag tgaaataaat caaggtattg actctcttat caaagaagat gttggagaag
     4441 tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt aaacatatac
     4501 ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa cttattgttt
     4561 atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg acactacttc
     4621 gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt tcgttggata
     4681 aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg tttaatttat
     4741 caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa caactaacat
     4801 atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat gttacaccaa
     4861 atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac ttattacgaa
     4921 atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta caggaggatg
     4981 attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa aaacttgagc
     5041 gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt ggtagagctg
     5101 ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca ataacagctc
     5161 atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga aatttgtaag
     5221 aatttaagta taaaaa
//
LOCUS       DAYAOY010000263         4636 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.184, whole genome shotgun sequence.
ACCESSION   DAYAOY010000263 DAYAOY010000000
VERSION     DAYAOY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.184"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            111..803
                     /locus_tag="ACGI6B_004145"
     CDS             111..803
                     /locus_tag="ACGI6B_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9753491.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            800..1735
                     /locus_tag="ACGI6B_004146"
     CDS             800..1735
                     /locus_tag="ACGI6B_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9753492.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            1883..2806
                     /locus_tag="ACGI6B_004147"
     CDS             1883..2806
                     /locus_tag="ACGI6B_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9753493.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            2790..3560
                     /locus_tag="ACGI6B_004148"
     CDS             2790..3560
                     /locus_tag="ACGI6B_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753494.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            3570..4352
                     /locus_tag="ACGI6B_004149"
     CDS             3570..4352
                     /locus_tag="ACGI6B_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9753495.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT         1474 a          704 c          909 g         1549 t
ORIGIN      
        1 aactactatt tcattgagga acatattcta agtgatgtca agatactaca atatgactaa
       61 attcctagtg ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag
      121 ttttaattat agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg
      181 aacagattga agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac
      241 aagattatca gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta
      301 tggaacaaat acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata
      361 attacagcaa agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg
      421 aaattacaga atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc
      481 caaaaaatga ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc
      541 atacagttac taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc
      601 tttactgtgc aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt
      661 ggggtgagcc atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa
      721 aaaaaattga actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt
      781 atcaattcaa cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt
      841 gctgtcgatt ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta
      901 caagcgccta aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg
      961 taaaatacta attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat
     1021 tgtctacgat tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca
     1081 acttatgaca agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct
     1141 ggatgctgta cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc
     1201 taggcgtaga gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt
     1261 gaactctgat gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa
     1321 tattttgaaa gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat
     1381 tccagaaaat cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa
     1441 tctggttcaa aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga
     1501 ggattccaga atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa
     1561 tttaccacat atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag
     1621 tggtcttggt ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt
     1681 agaaagtgag attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg
     1741 tttttccccc atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag
     1801 gttaatacaa ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa
     1861 acaaaggagg cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa
     1921 gcaatatggt gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat
     1981 ttacggattg ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg
     2041 tctaacaaaa ccaactactg gagaaattaa aatatttggt caggacattc acaataacga
     2101 gaagaagctt cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct
     2161 gacaggaaca gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc
     2221 agtagaatca gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca
     2281 atactctctt ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca
     2341 actgttgatt ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag
     2401 aacgtttatt cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat
     2461 tctttctgaa attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt
     2521 ggaggaagaa agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt
     2581 atctgatgca gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa
     2641 agtagcagat aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa
     2701 tcgtgctttc attctaaaca acattgaggt atcagaatct catttatgcg aagatactct
     2761 tgaagattac ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg
     2821 aattttcaaa actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat
     2881 ttccaattcc cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca
     2941 aattagttgt caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta
     3001 gccaactatt ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag
     3061 tgcaaaaagg aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct
     3121 actctgttgc agggttagga gctactatca ttggaggttt cattgttgga atagtagagg
     3181 gggttgctgt aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac
     3241 ttcctgttgt ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt
     3301 cttttgtata tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa
     3361 ataacccaat attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt
     3421 ggggagatcc cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg
     3481 cttgtgtagg tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat
     3541 acaaaaagca ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa
     3601 aaataaaaag atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat
     3661 tggcagtttt tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg
     3721 caaacggagt tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg
     3781 gaggatatct tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc
     3841 ctatttcatt acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat
     3901 tgttaagtct tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca
     3961 atatatctgt tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt
     4021 gttatgttgc tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga
     4081 cgggtgtagg gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc
     4141 tgacagactt ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg
     4201 gtactgttta tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa
     4261 ctattctact tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag
     4321 ggataaagca taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt
     4381 gctagacagt gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa
     4441 gctacagttt tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat
     4501 cataatactt gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat
     4561 tacattaagt atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt
     4621 aaataaaaaa aataat
//
LOCUS       DAYAOY010000264         4484 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.185, whole genome shotgun sequence.
ACCESSION   DAYAOY010000264 DAYAOY010000000
VERSION     DAYAOY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.185"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            284..1384
                     /gene="vanG"
                     /locus_tag="ACGI6B_004150"
     CDS             284..1384
                     /gene="vanG"
                     /locus_tag="ACGI6B_004150"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFG9753496.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            1381..2187
                     /locus_tag="ACGI6B_004151"
     CDS             1381..2187
                     /locus_tag="ACGI6B_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9753497.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            2205..4343
                     /gene="vanT"
                     /locus_tag="ACGI6B_004152"
     CDS             2205..4343
                     /gene="vanT"
                     /locus_tag="ACGI6B_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFG9753498.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         1636 a          500 c          784 g         1564 t
ORIGIN      
        1 ttccgattgt aggaaatttg taagaattta agtataaaaa aataggaaat tctttaggat
       61 aaataaataa agagcactct tgttattgat ataattactg tatcaaaaca agagtgcttt
      121 tattttgata cagtaattat atcaatagaa aaatgtgaaa atatattatg tggaacagat
      181 gcaattgtaa gtgaaggttg attggatttg aaatagaaaa agtattttaa catgtttctg
      241 tagattgtat aaaaataaat aaatcaacaa aggagaatca aagatgaata ataaaaaaat
      301 tgcagttata tttggtggaa actcaacaga atatgatgta tcattacaat cagcttttgc
      361 agtacttgag aatttgaata cggaaaaata tgatattata ccaattggaa ttacaaggga
      421 gggtaattgg tatcattata ttggagatta taataatatc cagaataaca cttggtttga
      481 aagggatact gatataattc cagttattgt ttcgcagaac cgtgtcaaaa atggtattat
      541 tgaattttta attcctgatg ctgtttctca gaatcatatt ccaaatagtg ctattgaatc
      601 atgtgaaaat gatattaaat acactaaact tgattttgtg tttcctgtac tgcatggaaa
      661 aaatggagaa gatggaacag tgcaggggtt atttgaattg gcaggtatac aggttatagg
      721 atgtggtact ctttcttcag cattatgtat ggacaaagac aaatcacata aacttgttca
      781 ttcagaaggt attaaagtac caaaatcaat attactaaaa tactcagata aatttaatgt
      841 ggctgaactg tcaaaagact taacttatcc attatttata aaacctgtgc gtgcaggttc
      901 atcatttggt attagtaaaa tttataaaaa agaagagtta caagtagcag tagacttggc
      961 ttttgaacat gataatgaag taataattga ggaaaatatt gatggttttg aggtagggtg
     1021 tgcagtttta ggaaacgatg acttacttgt tggaagagta gatgaaatag aattgtcaaa
     1081 aggatttttt gattatacag aaaaatatac attgaaaagt tcaaaaattc atatgccagc
     1141 tagaattgat ttggattgtg agaaaagaat acaagagaca gcaaaaatta tttatagaac
     1201 tcttggatgt tctggatttg ctagagtaga tatgttttta actccatctg gtgaaattat
     1261 ttttaatgaa gtcaatacaa ttcctggctt tacttctcat agtcgttatc caaatatgat
     1321 gaaaggtatt ggattgtctt ttgaagaaat attggacaag ataattgagg tgtacataaa
     1381 atgatgaata atattttatt agataaaaat aaagaagata aaatgacaaa tactatctta
     1441 ttgactaaag ataaagttta tgatgggaat ctaattctag taaatgcttt tttaccagtt
     1501 aaaacatctg aagatataga tttaattcct gtagatacaa ggtttccaag tattcttatg
     1561 aaacgtgaag ctacaaacat tttacaaaat attttaaaaa gtatatgtgg aataaatgaa
     1621 atagtacctg taagtggata tcgtactgca gaggaacaac aagatatata ttcaagttct
     1681 ctgcgtgaca atggaaaaga ctttactaaa aagtttgtag cacttccaaa tcacagtgaa
     1741 catcagacag ggcttgctat tgatttaggg ttaaagaaag aagttataga ttttatctgc
     1801 cctgaatttc cttataatgg tatttgtaat gagtttcgta aggcagcact acattatggt
     1861 tttatagagc gttatcagtg taaaaaagaa gaaataacag gaatatctca agaaccatgg
     1921 cattttcgtt atgtgggata tccgcattct gagattatca ataaaatgaa tctcgctctt
     1981 gaagaatata tagaatatat taagagattt tcgtatacaa attgtttaaa aatagaagat
     2041 aaagaaaaaa ttatagaaat cttttatgtg cctattcata ctaaagaaga agtgagaatc
     2101 tctatacctg aacaatgtgt atatcaaata tcgggtaaca ataaggatgg ttttattgtg
     2161 acattttgga gaaatagaaa ggactaagaa atggaggaac aagaatgatt aggaataaat
     2221 cttatgcagg gcttgattat tttaggatta tagctgcatt acttataatt gcaattcata
     2281 catctccatt attatcatat agtaaaatgg ctgactttgt gttaactagt ataattgctc
     2341 gtattgctgt gccattcttt tttatgctat caggcttttt tctaatttca acatataatt
     2401 ataattgtaa taaactgaaa tcttttctaa aaaatgtatt atttatttac gggatagcta
     2461 ttgtaattta tattccctta aatatttaca ctggatattt ttccatgaat aatttagtgc
     2521 ccaatattat taaggacatt gtatttgatg ggactttata tcatttatgg tatctacctg
     2581 catcaattat tggtgcaagt attgcatact ttcttgtaaa aagactaggt ttaaagtact
     2641 cactagtaat cactattgtt atttatgtaa ttggtatgtt tggtgatagt tattatggtt
     2701 ttttagaaaa aataccacta ttaaagaatt tatatgggta tatttttgaa gtatcagatt
     2761 atacacgaaa tggtatattt tttgcacctg tatttttcat acttggtgga ataatatcta
     2821 ataaatctaa ttatatgtca actaaaaata gcttgattgg atttcttata tcattcttac
     2881 ttatgttatg tgagggaatg tttttacata aactaggggt acagcgtcat gatagcatgt
     2941 atattatgtt attgccatgt atgtatttct tatttacagc cttaacattt ttgaaaggtt
     3001 ctcgagctag gttattgcga acatcatcat taattattta tattatacat cctatgatga
     3061 ttgttgttat tcgtatgttt tctaaaatat tcggaataca aactgtcttg gttgacaata
     3121 gccttattca ttatatagtg gtaagtatag catcttttat agcttctata attattatat
     3181 tgattcttaa acctctaaaa aaagacttga aaggagaaca taaggtaaat accaatagag
     3241 cttggattga aattgattta aataatttgg aacataatgt aaagatgata caaaaagcta
     3301 tgccagaaga ttgtgaattg atggcagttg taaaagctaa tgcctatggt catagtgctt
     3361 ttgaagtagc gacttgtgca aaccaaatag gtgtaaaagc atttgcagta gcaacaatag
     3421 atgaggggat tgatttacgt cgatatggaa tactaggaga aatattgatt cttggatata
     3481 ctaacccaat aagggcaaag gaacttcata aatacaatct tacacagagt gttattgacc
     3541 ttaatcatgc tatctcttta aacaaacaga aatacaacat aaaatcacac attaaaattg
     3601 atacaggtat gcacagactg ggttttgatg ttaatgatgt gaggtcaatt ttaaaggcat
     3661 ttaaattaaa atacttagat atatgtggca tatttacaca cttgtgtgtt tcagatagtc
     3721 tgaaagatga agatgtagac tttacaaata aacaaattga atgtttttat aagttaattg
     3781 attttctgtt aaaaaaaggt ataaaaattc ctaaagtaca tattcaaagt agttatggtt
     3841 tttttaatta tcccaatctt aagtgtaatt atgtaagaat tggaattgca ttatacggga
     3901 tattaagttc tccaaatgat acgactaaat tgcaacttga tttacgacct gttttatcct
     3961 taaaatcaca agtaattctt attagaaaaa tacaaaaaga tgatagtttt ggatatggaa
     4021 gagtctttat tgcaagcaga gatagcgtaa ttgctatatt atctattggc tatgccgatg
     4081 gtatacctag aaatttatcc tgtggaaaaa gttacgtaat aataaatggt tgtagagcag
     4141 caatagtggg tcgaatttgc atggaccaac ttgctgttga tattacagat atacctaatg
     4201 tagaagttgg taatacggca ataataatag gaagagataa tttatctgaa ttatctgctt
     4261 ctgaagttgc aaataactca tgtagtatat cgaatgaatt attgagcaga gtaggaagaa
     4321 ggttaaatgt tataaagaag taactcaatt aaatagtaat atttgttgta ctgtgtaatt
     4381 tattttactc tgactagaca tttttagtaa tattatgtct aatatatatt ttttataact
     4441 tacatgacaa tttaagttat aaaaaggtga tgtttcagat tttt
//
LOCUS       DAYAOY010000265         1229 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate 22S0371
            SAMN40601682-rid21575653.guided.237, whole genome shotgun sequence.
ACCESSION   DAYAOY010000265 DAYAOY010000000
VERSION     DAYAOY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601682
            Sequence Read Archive: SRR28438776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157532.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157532.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:15:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,066
            Genes (coding)                    :: 4,021
            CDSs (with protein)               :: 4,021
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 7 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 17 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601682-rid21575653.guided.237"
                     /isolate="22S0371"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Portugal"
                     /collection_date="2020"
                     /collected_by="National Institute of Health Doutor Ricardo
                     Jorge"
     gene            105..1058
                     /gene="blaCDD"
                     /locus_tag="ACGI6B_004153"
     CDS             105..1058
                     /gene="blaCDD"
                     /locus_tag="ACGI6B_004153"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFG9753499.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          524 a           99 c          208 g          398 t
ORIGIN      
        1 agtcttgttt cttataagta gaacctagat tggagtatat tacaaataat gtctactatt
       61 ttaatagtag gttataaatt taaatatggg agatgatata aattatgaaa agaaaaaaga
      121 attttatttg gatagctatc ttattggttg gtgtagttat ggtaatgtat tatgtaggga
      181 aaaaacataa cgatataaat caaaaaactg ataagaatta tataaaatct gaattaaata
      241 agtctgaatt aaataaatcc aaaattgaaa acaataataa aaacaaaaaa gaaaaaatta
      301 atatggttga ctatagtgat tgttttgaag gtattagtgg aggagctatt ttttataata
      361 ctaaaaataa agaatataat atatataaca aagaattaat tgaaacaaga aggtcaccat
      421 gttctacttt taaaatagtt tcaactttaa ttggtttaga aaaaggggta ataaactcaa
      481 aagaatcagt tatgggatat gatggtacag agtatccaaa taaaaattgg aataaaaatc
      541 ttagtttaga agaggcattt aaggagtctt gtgtttggta ctacaaaaaa ttgatagata
      601 aagttgatgc taaatctgtc caaaacattt tagatgattt aaagtatggg aattgcgata
      661 ttagcgaatg ggaaggagat ttaaaaaatg gaaagggaca tttaaatgga ttttggttag
      721 aatcttcatt acaaatatct ccaaaagaac aagtgcaaac aatggcaaaa atatttgagg
      781 gagatacaaa ctttaaaaaa gagcatatta atattttaag agatattatg aaaatagatg
      841 taaatgataa aaacataaat gtatatggaa aaactggaac aggatttgat gaaaaaaata
      901 aacgtgtgga tgcatggttt gttggtatgc tagagcgtga aggtgacacc tattattttg
      961 ctattaaatc agatgattcc aataaagaaa ttacagggcc aaaggtaaag gaaattgcaa
     1021 ttaatataat taagaaatat tatagtgtaa gggaataaac ttaattttat ttgtaaatat
     1081 tatttaccta tagtctgtaa tatttattaa ataattttac taaattgagt ctatatagag
     1141 caaaactcct ttagtacata aaatacttgc tattgattat taaattgaat acctatagat
     1201 ttaaagttaa taccttttaa agactatgt
//
